LOCUS       DAOQJF010000001        40019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_423_length_40019_cov_5.641928, whole genome shotgun sequence.
ACCESSION   DAOQJF010000001 DAOQJF010000000
VERSION     DAOQJF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 40019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40019
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_423_length_40019_cov_5.641928"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLF85_00005"
     CDS             <1..251
                     /locus_tag="PLF85_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE18061.1"
                     /translation="GEKMVRALPPAFVFCGGADPILDDTLRLAAALKRRSVRTSLRIY
                     PDRHHAFHAFVPLDPEARRCWRDQIEFLEEIGREYGAA"
     gene            complement(400..852)
                     /locus_tag="PLF85_00010"
     CDS             complement(400..852)
                     /locus_tag="PLF85_00010"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE18062.1"
                     /translation="MPQLTNEIFIAVKPRIVFDFVTRPDLWHEWHPASTSAKLPRVPL
                     HEGDEFDEIITVTYPLVKVQRPTRYRVTLSRRADAFEVQGNSSLFDLTIHYDLEAHDG
                     GTRFVRTLTYEVKGPLRYFEPLVVRPRIVGQSATALANLRRLLENQGS"
     gene            complement(887..3226)
                     /locus_tag="PLF85_00015"
     CDS             complement(887..3226)
                     /locus_tag="PLF85_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNE18063.1"
                     /translation="MNGLCRIILLLAVALSAACKEYSTTELLNLQWPTGGARSLKITS
                     PSPITVDQPFTVTVTATNTAMPQGIQQDYQNPVNITLQVGNGTIASVNGGGWYLGTQN
                     FTVVYNNPALVSNASEVILLRVTDPKDSMLTTVSNNITATAQAVFNQFKISAPGAVFK
                     GQPFNLTVTAANSDNSTYTAYSGTVNLSTYLVAGTISPSTVTGFVNGVATVSVTLAQS
                     YTNLQIRAVDTVNASITGLSNNFQVYFDSVSLAAFAQSATSIRVTWHFPTGTTQALVY
                     RDSGSGYQLLSTVFLPTTNYTDTTGLTAGQTYPYRVVVQDNLATTQYQATTTAVPRGC
                     ATSVSGTVGTTTWTPAQSPYCVTGNISITGTLTVAAGTSVLVNPTFSINVASGGSLVA
                     QGTLTAPIVFTSANASPAPGDWAGLNFASGSIGSTITSDNYSGGSLLQYTIVEFAGPG
                     VTTGESLFFDSCTIQNNRTTTTNGAGISANQAAGRQVVLKNTLVLNNTVATNLYGGGL
                     YATQRAAIYSSKFFNNTITHAANFLRGGAAYLGGDNNLVTSSQFINNSLNSTQSNHQG
                     GALFFNGDFNTITGNTFQGNLVSLINTANGAAAYLMSNNNTITNNLFTGNTATAVTAG
                     SYGGALVVNGFGNTLTRNTFSGNTSTGTNSYAGAVSLGNNNTLVYNLFTTNAATRGGA
                     IFSNGTTATVNHNQFVSNTGTNGNNLFYGAAGTLNFTNNYWGGATSGLTAMGICDGSN
                     TGGSCATSSGTIDASGAVATPWPLCKTAPSDPDCVGANF"
     gene            complement(3312..4196)
                     /locus_tag="PLF85_00020"
     CDS             complement(3312..4196)
                     /locus_tag="PLF85_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE18064.1"
                     /translation="MRHLALPDLMLVLCTVIWGGTFPAAKIALQQISPWTVVSLRFMV
                     AGSLFAAIYYRQVVRADFRLMLRGVALGLFFFAGFSLQTFGLVFTSSSRSAFITEMLV
                     IFIPLMYFVFYRRTPSVYTVTGIAVVLLGLFLLTSPGDDLALNRGDWLTLGCALAFSA
                     YIIGVSAWSTPETRGVLSTLQCLTVAALSLPGMELHNSNLAAGHEPWLALAYLAIPGT
                     VIVVLLQMRFQPLTTPSRAGVIFALEPVFAMVYAILLGLEAAHVQGILGAAVVTAGVF
                     WSESGHLFIMKKRKAPAP"
     gene            complement(4217..6973)
                     /locus_tag="PLF85_00025"
     CDS             complement(4217..6973)
                     /locus_tag="PLF85_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE18065.1"
                     /translation="MTLKELEIFGFKSFADKTKITFSTSVSGVVGPNGCGKSNVLDSM
                     KWVLGEKSVKTMRGEKMEDVIFAGTSSRKAANFAQVSLTFDNRMRLLKLDTDDVKVGR
                     RLYRDGQSQYFINDSRVALKEIEDLFRDTGIGKSSYSFMEQGRMDQILSSKPEDRRLI
                     FEEAAGISRFKAQREEAEKNLENTQLNLTRIQDIQRELERELKIKEAQAKKTAEYNAL
                     MAKYKEHDLKIRYLMVRDANDKLEDLKEKLARRHAEKEKIQQKLIQMQERLTGLDEEE
                     RSLKEELHKKDVTSQVANETIARHNQAISEKEERKTSLTAQLAELKEQIGRFEKRIKE
                     LKKEAAEQNQFTLQLDLKVSDIEKEMSRLQKRIEECAAESATADEELKKLEADKSAAE
                     KSLSEVQGRYEETIRELLLSLKTEKSRWEKTAKNREQAFTEIELYCNETEATLTAVLA
                     SPDDHLPQMRKDLQRLKDSKVFGKISEKAHLLAEMSRGLYEILFEKGGVHSRKEELDE
                     QIAALEQRIAAANRETQRLLARKQQLAEESAASRQERERLNGDIKSLNVQITSVKEKE
                     KNLAQQIVNEETNMGFVRSQFTEIDRSLVQLSQEQKNLTREVEKLKSSIEKETQRIAG
                     IERDLKKADDKRRELVLTMKSESGKTDEVFVAINELEGKIGALGGTRDTLLQNIYNDY
                     SLTWQELSGQFTSGKLILADEKEKLTEIQRGISALGQINQLAIEEHRHIKEMYDHQQA
                     QVDDILKAQEDIKKVMAEIQGKSEALFRESFEQINHNFAEIFAKLFNGGQAKLDLLEP
                     ERPLASGIEIEATPPGKKTKSMRLMSGGEKAMTAIALMFAIYMVRSSPFCVLDEIDAP
                     LDEQNVGRFLTLLDDFAKNTQFILITHNKKTMGKAQVLYGVTMNEPGVSTLLSVELSK
                     ATTG"
     gene            7041..8744
                     /locus_tag="PLF85_00030"
     CDS             7041..8744
                     /locus_tag="PLF85_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNE18066.1"
                     /translation="MATGELTGKYAHYHFGYEEGRFSIFADTEKLKGISLVSTRDPAR
                     EAERHIGAFRLKPGMLAVVAGVAHLPMLEMLAEQLRSPEGKGGVILAFEADTELYLSL
                     RKARPDIFADVAVIVPETLSVLTELVEKIDVENFVGYRVFENRPSIDLAPPFYSGAVT
                     DFKKKLASRFSDLFTRLEFEERWVFNSLSKLPSLSSMRPVSALFGLGAGAKALIVSTG
                     PSLRDSLAEIAANRERYFIACADSAYRVLVRYGITPHLVFSLDSQAHTAKHFGLLPRG
                     KKGAFPILVCDAVSNPVVARDWRGEICLSFTAQYPDDRRVVSPGCDYLEEGQFFAGTP
                     GLIPGDLQSGGSVATSIFDLMRLMEFDEIILAGQDLAYTRREIHTPGTHHTDEWLSKT
                     TNRLQPLENINERVLRRRHTKREVSLTGRPIVTDYILSLYRDWFVNAMQAVPGKTVNA
                     TRDGLQIPGAKQKETIMTETAGKNQEILQKLLAAPPMVTYPQRVTALKDKIQGANREN
                     PERDLPFLRYVGRKYSIKALRSPEQARRLKEKQLVKQRNFLRKMASKLQLSPAETGVV
                     R"
     gene            8746..11178
                     /locus_tag="PLF85_00035"
     CDS             8746..11178
                     /locus_tag="PLF85_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNE18067.1"
                     /translation="MRPRFSTIFWPFWLYHVRRHAWLMLVLLFLTSFIGFRYGRQIRI
                     DTNLLNLLPSNTESLVRLTQLRDASDVKGFLVATFESETARSERDLTAMAERLEATIA
                     SQDFLRRSTSRVIYGISGEFLLRYSLWYADYGDLVKVRDRLDNSIKQAKRRENPYFED
                     LEETAPAPFYIGDVLNKYRKKYLSSGAFIDNDRKRIAVLLSLREPPDNIQFAQSYLAA
                     LKDSIRFEAEKRQFRLHLSGRYASDVEKQRRLAEDISQSTTITLLILIASLVIFFRSL
                     RVLLVVGLPVMAALGYTYWAAWYFIGQISIISSFLTSILLGLGIDYGIHLFARYKGER
                     VKGHGMTESLEITMRNLFRGLSFGMISTAAVFLTLSFSRFIAFAEFGRIALGGILFFF
                     SAFVLYFPALVFLTEKYSFGTTEASHILEKTRRIRGWQMAIILFIAVYGAITALRPPF
                     EYDFFELESTDHRRTKKDGQHGQIRIIENREHMVIYELPNLEALRATEQNLRTLTAGS
                     GLELHSALDLVPTEVTRKQAVIRQINALLKQAELYALVSLEGATLTRIRQGLRMTEGR
                     PVTLEDIPEAFRQHLIQNGKYYLYAFPREVSDLRRGALDFAAKVRETCSVVLSVGATC
                     PPEKVVRGISDLYVLDDILNTVMRDFAKQIALIAAVIAVIVLSMTRRLAGVTLILAPL
                     AAGILGLFALIGAFHYLSATWLFELNYINLLAIPILLGVGIDNGIYLYSHARESGLSQ
                     VNQIMASTGGSIFISNFTTSMGFLSLTISSHRGLASFGFLTFAGMLCIFYAYRLLFPA
                     LARWLQQAEAEI"
     gene            11175..11717
                     /locus_tag="PLF85_00040"
     CDS             11175..11717
                     /locus_tag="PLF85_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE18068.1"
                     /translation="MIRPLLPLLLSSLCWAQTIDLKSEKESLAKIDAAAKAGDDRALV
                     TECDRLLAVHQVTALAWYTCGKHLLFVKTTDEKQSLANAKTAYGRLARAVADFSRTGK
                     QTFYALDALQYQGLAALVLHDFDRAMVHFRAALARDNRIAATWYNLGVLYEMRGQQEE
                     AMRSYDRYLRLKGTGDSQEF"
     gene            11779..12189
                     /locus_tag="PLF85_00045"
     CDS             11779..12189
                     /locus_tag="PLF85_00045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE18069.1"
                     /translation="MKVLRRQKNLSFGTSPDNEQGLKLDLRFDEDTKTAYGEIVLPIH
                     FQGNQPECVHPGVIAVMLDEVMMKINQALNLNVNTGEITIRYLQLAKVNEPLYLRGYF
                     VKKNKRVIENRAELEDDIGKIVARGKGKYIEQDL"
     gene            12257..12339
                     /locus_tag="PLF85_00050"
     tRNA            12257..12339
                     /locus_tag="PLF85_00050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12290..12292,aa:Leu,seq:caa)
     gene            complement(12382..14100)
                     /locus_tag="PLF85_00055"
     CDS             complement(12382..14100)
                     /locus_tag="PLF85_00055"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNE18070.1"
                     /translation="MIPDRIITNPLLEIRLKGSQAQMGAQYGEIVGSQGGYEPLLDFY
                     PRMAESLLVSGMPRRMRKGPVRRVAAQLMNWAVNSMVKNRLPQFTERAHAMARAANMS
                     PDMARHMLVMDVFQNSIGVLGRLGALDVFDKPLVHALGACTSAVVFNSHSADGELMHA
                     RNFDFPGVAVWDKSPTIVYCSPDHGIPYGYLGARGADVPGITAFNAEGITVTVHTRFH
                     RDVDFAATGVIDLGHEIISQARTINDAIDIVSRHKVASTWGIVVTSAKEKNAAIIETT
                     AKKMRVTWARDGYLGNTNHYQHPDMVQGEIATSNGWTSYTVDRLRLLDKFFAEMQAKG
                     GATLTDMQHILGADHEVDALGSPRMMGSLIGYVMSVQSVVFKLQSGLISLSVGEAPTG
                     WGPYINHEINWSGDALRVVDPAKEQLSAVTASYGSGPALEAYRRFQNAYLTDFNCGSL
                     DEVRADLSKASAYAVEDGSLKFVEGVFALEARDTERAYRHLMQAVALEKGPYRKGQAL
                     LWAARAAAASGKTAEAASLRREIFALSHHNLAEFKRLAERDANQLITKRDYSDVVLSM
                     SMLDAA"
     gene            complement(14137..15693)
                     /locus_tag="PLF85_00060"
     CDS             complement(14137..15693)
                     /locus_tag="PLF85_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNE18071.1"
                     /translation="MNSRSLLYLLALILAYSVAPLAAQQKRAKKPTAAKPQPAPQKPE
                     KPAATRVEAAEEDAADAKSTAEAETKIAPPPVLVTPPAVEAEEPQNHHYLRGGLMATN
                     TYYIDPVINPGLFAGFYRPFAKYSYNDKYSGVARGLLTVKHYVEPVGALQQTKVTGSI
                     ELLAAEAKYDRHVFTAGRSFYMIEQGLLFANFADGFGYNGRFPFGQLRGFATYSADYG
                     TSACALNVTGCNGDAGPFVNTPNLLPDSGIQNSGRRVFFAAEYFTPEYAGSQATAYAL
                     YSRDMISEDTSRTKYAYHPWYGGLGLRGYIVNSNLRYRADIIYQGGSTYNLVLNGVSE
                     AATISAMAALANVTYNLPVLRDYDSQLTAEVAAGSGDADSSRMTNASQSNTEGSYTAF
                     HNFGSYSGGLALKPRLANMQIVRLGTYFRPLKPNFTLRNLAVQLKYSYYRKSVATGGI
                     SDPAATQASNDVGHAGDLALTFNPMADLQFFWGFGIFKPGAAYPQLNFDGTDGQAWRY
                     AHLFSLTLIF"
     gene            complement(15700..16341)
                     /locus_tag="PLF85_00065"
     CDS             complement(15700..16341)
                     /locus_tag="PLF85_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18072.1"
                     /translation="MKSLFRQTLSLFILISALAAQPLTENLILDEELSALRAITITKK
                     ATFSDVCKLVLIHRNELEKFTTDEARCARLAELGIYDTAAVGRIHLKPATKGAVAKAV
                     LNLYDLEKTLLFRLTGSEWYAIQNAEVLSLIDKGSNSWDELSGEELVTIMDIAMQRAG
                     EKASWMQPKNPYRDFGYETYPEMDQKTKPLEPEAKGEEKSDGTRPTPAPAEAR"
     gene            complement(16355..17914)
                     /locus_tag="PLF85_00070"
     CDS             complement(16355..17914)
                     /locus_tag="PLF85_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNE18073.1"
                     /translation="MKFTKAVACGGAILTLLATIGCAKKDLPEARVNGAAKFQLNGGA
                     AISQASSDEYNPYVIQMGDNYLVLVFGSNRACGGCTGHNLFMAISVNAYNNDAVFPAF
                     ENPTVLTIGGTPLNYANRIQYAATATGNNVRIFLTNTGGNVQQTGAIPPTGPYNTTLT
                     NIANVAGLTSTVLGVEYTGNRVYARQGGTVYAVNHASAGDALVAMATGQTATSVASVE
                     GTFTSRTDGFFSLIDGTITSMSLYGNGGNLASVNNAIAKGRITARNVTVMGGLGGGSN
                     GALMFISGTESGGTSEDMYVVDGITVREMWQQINPKPPAAPATQGGGGGTAAIPTFSP
                     AAGHYAMPLNVTMSSATSGAIICYTTDGATDPACSATPACTTGTLYSGAVSFYNLTRN
                     FRALACKLGMANSAVVNATHVSDAMPPTTPGSPFADATSTSQVQIMWSASSDAGTSTA
                     QLVYEICQTTTSGNCSTLTATFTSSAGATSYYSNGLTGCTTYYYRIRARDLAGNISTG
                     TTQVSATTSGC"
     gene            complement(17923..19110)
                     /locus_tag="PLF85_00075"
     CDS             complement(17923..19110)
                     /locus_tag="PLF85_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE18074.1"
                     /translation="MSTYISVLLLFAFIPVSVMAVDKSPQARFLTLKGKVQVFEAGRW
                     VKAAIDSRLSEENSVQVGYRSAAVVALPEGSQIALKENTVLSFNELATDLSKPRTELF
                     IQQGGLGAYVKKPAGAQKNTFLVKTPTVVVGVRGSFLDLQRHGTQHEVKAIESAAFVR
                     NKQTATTNRERLQKAFAALERAYLIDRDEKNRAALLAEKAASDDDKQAFIALQQSATD
                     APEFVQAVKEIKDHRTLTRELLVRQEKVLETNQANRKDIIQALMQVRALTRLQLLWAE
                     AAYKAELEAYMVALKQTNRKKEQADEDDELFFNNLEFASVPQGDAARVSGDLQGPYST
                     RTFDSRAERSFSSGSSAPEQSFLGNITDIQSGFGNDIQGLYNSINSVTQPSSVGEPTL
                     QKF"
     gene            complement(19210..19965)
                     /locus_tag="PLF85_00080"
     CDS             complement(19210..19965)
                     /locus_tag="PLF85_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18075.1"
                     /translation="MKILLANGFIALCVVFSGGCRSAPSTNGEQNRRLTLEEFVLLDW
                     QRQVEYLEQVASRGTSGDDDRIVAYALTDDDTGVIVKALEVSHALSLKEHLPRAEELL
                     LNNDAIIRWRALLLIEKLHGNEAVLPQVARLTGDKEWLVRETAYRVLRTFSSERKKRT
                     YFYTVLFRLNEKNPSVVAEIYRTLVWYDDESAWPYMIKRSYHCKSASELILVMRELAR
                     TKNREAQVRLKTLTRSQSIIVRQEANDLLREYF"
     gene            complement(19962..20711)
                     /locus_tag="PLF85_00085"
     CDS             complement(19962..20711)
                     /locus_tag="PLF85_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE18076.1"
                     /translation="MRRLIGIIAAGLCLAGAPQARAAIAPWEIAGVATGALGIGVTEL
                     YKKDLVPDEARWKTPPAIDASVRSGLVWSNTRLASTLSDVMLYGVMPTTAFLLPLATN
                     HDYSRAALTIAEAAVMTGVVTQMAKFSFARARPYAYYGNDYSNPDSKLSFFSGHTSYS
                     FALSLSSAMLLAESYPRYSTLIYSVALLVAMLPGYLRIAADKHYFTDVLTGAIVGSTI
                     AYGVVHLNMTSNSMQYGDERRLVLQRMFVLQ"
     gene            20837..21472
                     /locus_tag="PLF85_00090"
     CDS             20837..21472
                     /locus_tag="PLF85_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18077.1"
                     /translation="MSIQTPRFIDSLERRFHKFGIENLGLILVVMQAFGFLAYQISPA
                     AKAKFMLIPELLLRGEIWRAVTFIALPLSNDFWIIIVLFFIYSIMQIIEQAWGPFKTT
                     LYFLIGLGLSVAYSVATGLAIDTFMPLEMSLFFAVAALYPTTEVLLFFFIPVPMWILA
                     AFQALIVVYLFIISDWIQRGYYAVVYMNYFLFFGLHHFGQLKTWLRRRQWR"
     gene            21482..22066
                     /locus_tag="PLF85_00095"
     CDS             21482..22066
                     /locus_tag="PLF85_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNE18078.1"
                     /translation="MRRLTNFTAVLLLACGSVAWGAGKTYWYEIDLNRSQIAFLAKSR
                     IAKAQGLFRKWEFKGKISSNFHVVGDLAIDCASVDTDNERRDNHLRAADFFDCAQFTR
                     HTFRILSVKPDDKTPAKARRFDVSGELTIHGKTQPVSFELRREGDEKSFVLTGSTFID
                     REAYGIAYNSALNPIEKMIRIDIRLALVRKGDRP"
     gene            22066..22764
                     /locus_tag="PLF85_00100"
     CDS             22066..22764
                     /locus_tag="PLF85_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18079.1"
                     /translation="MAKKLRYKGLRITVSLVIVLGVIAFWVLRPLTETPMNTGDFTPI
                     RDDSLAVKFAPRFVPHALYGAPTRLLYRMARGRDGNIHIAYHPFYPHEENPHEGSGAA
                     LSRFIYTGGLHIKDIMFGPADIELVEVILDKNLVPVELGYEDAGDYNPKAFSVRHVVK
                     LVKKPQAPFCFTTSSWNHMFALARGELCRSVQGPAPEYFTEAGWQKYRMVKETEAMLR
                     RNRMHRVYERLAAP"
     gene            complement(22777..23430)
                     /locus_tag="PLF85_00105"
     CDS             complement(22777..23430)
                     /locus_tag="PLF85_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE18080.1"
                     /translation="MRFLLALLTCLPVAAVMADSRPEILDRIRDYDMRHSDLLVKIVK
                     DPETLRYAKVRALEKMSVIYRQSGKEGESVATRYLDGVSAGLQNKSPDVREAACTALI
                     VFSESSVAQQIVASAVKTLRDETHPQVIAACARSLRQYPKEAAVIVPALLVRLDKYLT
                     EFQDSTADERALRQICQALGVMKVRKSFIPLLKLLQSRYDEDVKSAAQEAIQAIRVQ"
     gene            complement(23441..24046)
                     /locus_tag="PLF85_00110"
     CDS             complement(23441..24046)
                     /locus_tag="PLF85_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18081.1"
                     /translation="MANGTMEQGKKPYFWQRKYFIDKRFQGKFVLRYSIVVAISAVIV
                     LGALFALKNKAYTLLPDGASVLAQMDVDNHKSLKKDKDGNWQIVPEGEGEEYFPLKVT
                     KGGFRFYNAFDLYLWPVVVVTFLNILIIMVYSVFFSHKVAGPIFKIKKHLNHYLATGE
                     FNDIKLRKGDNLDDVARLINHAIHRHEHRHDNDRRPENGQG"
     gene            24263..25135
                     /locus_tag="PLF85_00115"
     CDS             24263..25135
                     /locus_tag="PLF85_00115"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE18082.1"
                     /translation="MLEPAKLEAKLREIQLPFQPQVLAAVLQLRDDTRMDFIELDRLI
                     RSDQTIASLILKLANSSFYSRGNEIRTLPQAIGMIGFRTIVSIVTAASAKNIFLSGNY
                     AKFRRYVWQHSLVTAIIGKIICEKMQWKEAAEEVFIGGLLHDIGKVILNQIDRKLYID
                     VMNTTLDSGKAFRHAEKESFGVESPVVGQLILKIWNLPVIYREVTVFLDKPSDPAMQR
                     LPVHDQQIVRIVGLANLLAKINEFGHLETDHAALESEYLQPLGITPDGALMSIDWKKA
                     VVNDTYYQVFSVIG"
     gene            25137..26876
                     /locus_tag="PLF85_00120"
     CDS             25137..26876
                     /locus_tag="PLF85_00120"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE18083.1"
                     /translation="MKTDQETEPHDRLFEITAFELYSGIKNTIRDEIRRVVTDLGRRQ
                     LAGPVTSMVFELADNALKALYRHVFYKYAIAETGLGDLPYDAWLKVFRDEIETNSARN
                     FERICRQRSLAVVVRLTQLDEALRIEVINDGVPDKSEHEYLRNLLKKSASAHNIQAFV
                     DAENIDIVDTEKPGLGWTLIFLTLRGMGLTSSQFHFIIGHNQTTARIDLPLSVFRTTS
                     EAAVKVVDPGKVADELFSRVANTLNFGIVIFDRKGDLLEVSRAVLDQVGLKPEQVAEF
                     PELLKARFIEDVFSGPFNVASVYHFENYRLKVESKDGRKEVLFNISGILNRETQRVET
                     IWQTINLEQDIKTLSEGSVFENIHIQSIVRPYIPNMILEKAHETLRHGLFKLPNEHKE
                     LTIMFLDLIGFTSKSEHLDPAGVIDLLNLAMGIAVKSIEVHHGFIDKFLGDGIMAIFS
                     DPLEVVVAGFEIQNNFKGVNEYRQVNDEEAIEMRIGINTGMVILGSVGTKRRMDWTAL
                     GDVVNTASRIEKASRANSVLISEATLEKIRDHVTVAESFSQKVKGKQQEIMLHFLKSV
                     DYASAGQKRRLEL"
     gene            complement(26887..27162)
                     /locus_tag="PLF85_00125"
     CDS             complement(26887..27162)
                     /locus_tag="PLF85_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18084.1"
                     /translation="MLFFAILFFVLGCWLVIISGKLSGKAIEVESLLEDAGITRVLKY
                     IFTHNPFTGYQKDIRILLYGLAGILLMLLGVATAAYFLLVASDNSSR"
     gene            27236..28003
                     /locus_tag="PLF85_00130"
     CDS             27236..28003
                     /locus_tag="PLF85_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNE18085.1"
                     /translation="MTLKKKKIVNRAKSTRQPKKKAARQQASRQSARTKLADALKRRA
                     HHARFKRAMRHEIANLLTHGIGAGLAIAGTAFLIVKGVGLHDPWRTVSYSLFGASMVF
                     LYMASSFYHALWHPKIKQILRRMDHSAIFFAIAGTYTPILLVTLHGPLGWTFFGIVWG
                     LAAVGITFKMLFGHRYEAVSLTTYILMGWLVIFFVKPVYLGLSVGGVWLLFSGGVAYT
                     AGTIFYSMGRLPYHHMIWHLFVLTGSVLHFLCIYLYV"
     gene            complement(28031..29629)
                     /locus_tag="PLF85_00135"
     CDS             complement(28031..29629)
                     /locus_tag="PLF85_00135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNE18086.1"
                     /translation="MNLRESNIQKLQTRHFDVLVIGGGINGAVSAAALSGKGAKVALI
                     DKADFASFVSQESSNLAWGGIKYLETLEFGLVRKLCKSRNHLIRNYPSMVQEIRFFTT
                     IEKGFRFPVWFIWLGTWFYWLIGSFFTKTPRFLTKKRINQLEPVVNTTNAAGGFEYSD
                     AYLHDNDARFVFSFVRTAINNGCIAANYVEATGAQQKDGNWIIAARDTVKGKKIRITA
                     NVVVNACGPYADQWNRTAQIATEHEHVFSKGIHLIVNRLSKEPRVLTFFADDGRLFFA
                     IPMGAKTCVGTTDTRVTTPESHVTPEDRAFVLQNINKRLKLEKPLTEEDIIAERCGVR
                     PLVVKKGKGSGKDWVQLSRKHEIEVDKQNKQITIFGGKLTDCLNVGDEVSEFAEELGV
                     QLSFPKRRWYGEPHESVRDEFFHQARLMDLDSYTAPTSSEKLSTRFWRRYGSHALELL
                     EEIRENPEEAELLIEGAEYTRCEIKQAAAREMITKLEDFLRRRSKIALVTRKEQIRRS
                     KGLKEACKILFVKEADKRWREYFG"
     gene            29760..31784
                     /locus_tag="PLF85_00140"
     CDS             29760..31784
                     /locus_tag="PLF85_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNE18087.1"
                     /translation="MTGGRILFLGLLVMQVSCLRYGKILGIFVLLFSTNCSSDNLLET
                     SIKENINARGSAIIQNFTINRIYVSTTGNATAANLSFSLAEKGKYSVHTGSCQNSPPL
                     VPMTAIDAGNITAAISATQLQHGLNQLKLCYWESVRDMLSDNLGFDLTRDDAAPTVVS
                     TAPANNAYRVDTDTNVRITFNERVVLPADTSTAIQVSNNQGIVTGTVTFDNQTNTLTF
                     QPTTNFGFTKNIQVDLLAGIADLAGNAITPMTVRFATRFTIQWGTLGSDMAFDLAIDN
                     ANNLYLAGTAQGSLFGQPYQTSARDAFYIKYDPQGQPVFARMIGSTNTAGTGDDSFQS
                     ILVDNAGNILVTGESFGSSSFSFDGIPKIGSNDIILLRYSSDGTKLSTELIGATSNDS
                     GRVLLRNTANEIYLVGLATSSAMLIDGQPFLGSNDIILRKYDATGVKIFTRVIGTSAS
                     ESISGAALDSTGNVYITGGTLGGSFYGTNPDTNSDMYLLKYDSNGNMIWHRQVNNPGT
                     ERDAKLLIDGSNNVWQCGYATQGYDNNSTLGSADVVLTKYNANGDKLFSRQYGTERND
                     YCGSIARDSLGNIFISGSTSGNFGGPNADLTGTSSDIFLMRVDANGNMVWVKQFGGAA
                     ADEALVMKINSLDQIFVAGRTQSNLEGNTLLSASNFDFFLMKFDTNGNLY"
     gene            31784..32878
                     /locus_tag="PLF85_00145"
     CDS             31784..32878
                     /locus_tag="PLF85_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18088.1"
                     /translation="MLRLFWQRSKELTRFTVLLGLAGNLSAMPDIPKWEITPGIGMGY
                     STIQGAFDQFYKPGFDLNFSLFVPTGRWNSFVQPMASFGSFPFRTADSTKLTHYDFSV
                     PLGIYWPAFAGISPFVAIGPEFSYYTLAAASTGLSSTAYRIKPMASAGLLIPVNYFSF
                     ITVGAKYSYTQLSGEAFGNITLTAGFTVSYPTYRRNDEAVSKALAEQSRAQAIAAEVE
                     ALFKAGRTFIAEEKYDEAEQKFREMLALKPGDAEARREIDRARALPILKEARLYREEG
                     KGLSAIRSYEKAAVSLEEARTELTEFRQELKSSVPALTKSAIDAYNTGQYINAIVMLE
                     RIQAIDPENETVKIYLPRARNRQRAIDKLN"
     gene            complement(32892..35531)
                     /locus_tag="PLF85_00150"
     CDS             complement(32892..35531)
                     /locus_tag="PLF85_00150"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE18089.1"
                     /translation="MAAQARGEFQARLYARSVMNILLMDAGDVNSARVDARELSTIFQ
                     ALENEGLLRALAWLSSKDPALDGRIVGGFDRADNPVPDVFRAGFTPWESAAFAKVDCG
                     PKGERCLRFTGEAGPPGKPPLLYVELLYSLDEVLAPIIRLRGILYATVALVIALMLAV
                     GFYIAHRITRPVANLMLGVQNLASEGPHSEIPITDHDELGQLAGAFNRMAATIDKSMN
                     ELRKKNEELKQLDTLKDDFLAVTSHELKTPLNGIIGLSGTVLDGAAGPISDSLRGNIQ
                     AIRSSGIRLAALVDKILQVSELANKKVVITPMPVNIEQLLAEIRTLMAPAAEQKKLRL
                     VVQVAPGVPAVMADRDRLTQILFNLVGNAIKFTEHGEIKISAAMRDDRMRISVRDSGI
                     GIAAGDKERIFHSFEQVEKSDTRRYGGLGLGLSVSQQLAQLHHTTIHVESEMGVGSEF
                     WFELATAKVQPPVEVPETGILPLERLSESIAQEMQKRAATQVQQAETTPTPKRTYNIL
                     LVDDEPVNLQVLINQLSLVGYKVNVAESGKQAIDYMEHSPVPDAILLDVMMPGMSGYD
                     VSRYLRNKYASYEVPILMLTAKNRSEDVIRGFEAGANDYITKPIESEVLLARVRTAIS
                     LKESVREKDMLENLQRELSVAQKVQQALLPVRYPSLPGIRIAARYHPMAEVGGDFYDF
                     MEHEDGVGVLIADVSGHGIPAALIASMVKMAFSLNRHLAAEPARLLGALNQVLCDKVD
                     NHFVTASYYFVNTRTKKVYHASAGHPPIYFVERRSGLIREMKPKGGFLGVFSDMVHTQ
                     GEETLQDGLRLLQITDGVIEARSKAGAEFGAEKVREIIRKTLNQDTEATADQIMQDLR
                     EHNPAYFDDDVTFVLMDYHVPAD"
     gene            complement(35676..36440)
                     /locus_tag="PLF85_00155"
     CDS             complement(35676..36440)
                     /locus_tag="PLF85_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18090.1"
                     /translation="MQPFRLGVVNFLNAYPLWAALQHDSRVILVPDVPSRLAIELRAG
                     NLDAALISSVEYLRHPEGFAYHPGLCVAAEAESKSIRLFARNNDVSFREKIAQTELIY
                     TDVASRSSVAQLRVILHELQSSPQLVEVGDPAERIPVLAPGEALLSIGDTALRYMHSP
                     SYDLQTAYHTIFHHGFVYALWVGRDAIMHALQPVLDLAYQQWLNDQEKLLAEAVQRFG
                     FDRLFAEDYLSRIIGHRMTPEHQADLEFFSARFRQL"
     gene            complement(36458..37153)
                     /locus_tag="PLF85_00160"
     CDS             complement(36458..37153)
                     /locus_tag="PLF85_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18091.1"
                     /translation="MAHKILPHKGLVEIAPGLWQVEGSLPFPLKRNMTVVKLSSGGLL
                     IYSAIALDEAGMAELEKLGKPEVIIVPQPFHTMDLAFYKLRYPGLKVLGAEVGEVYNG
                     VTVEGDVVKGLNDPRVSATLAPGLKMHEIHLFVKIPGGNALVVCDLFAGDDCYATGLG
                     AKLAKLIMGTANGGFGIAKIVKWRQLADRPVVKKYISDLAADTSLKLVLVCHGAPLSL
                     NIGAGLLAAAGVL"
     gene            complement(37199..38143)
                     /locus_tag="PLF85_00165"
     CDS             complement(37199..38143)
                     /locus_tag="PLF85_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE18092.1"
                     /translation="MSKLSWEDQFMESIPAIVAGGETEERARQLLNDYIRLARTTAMP
                     EALDFEHHPELAATASPYLGRDEEIHDLMLAIVTPLLSRYKVHGLANFEKVIPMLTTT
                     GVTIVSNHLSLFDAAVVYGLLHREPHLTQYAEKIFFIAGRLVFTSDYSRVAGRMFHSM
                     LVASPRDMAENEPIKRELARLNIRSFKEGKERQRNGHILILYPEGTRSRDGRMGKFHS
                     ALYNYLEGTVVLPVAITGADKILHSNSFTFDITDGSMSIGDPIFVGPSVEAPQSIDHI
                     DTATLPKDTRKQDAMDAIALRLAALLPPAMRGAYAGSE"
     gene            complement(38214..38894)
                     /locus_tag="PLF85_00170"
     CDS             complement(38214..38894)
                     /locus_tag="PLF85_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18093.1"
                     /translation="MRAPSWLLSLGLILLHACNNYNLKDQLENPGGAEKFTDRLIVFV
                     SSSVTVGNMEALFASGCSGTGIQRADCYCQAKAREYNRRMNAGSRFVAWLSETGTPVN
                     NTMLCRILGTTGDGCAPAGSFTWYDTSFQPIVSGMSNLFNASPSFSAAIRLTESGNPA
                     PVVLDDVWTGTAQQGNAQPNTCSNWQDATGAIQAAIGDSSAIDFGWTNRSGGFCNVPR
                     RIYCFAVP"
     gene            complement(38992..39678)
                     /locus_tag="PLF85_00175"
     CDS             complement(38992..39678)
                     /locus_tag="PLF85_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18094.1"
                     /translation="MRNFVRGLSILLFAAVTACNNYDLLDSLERPGSKESFSDRLFVF
                     VTSQMTAGDMFALNAGSCGGTGIGKADCVCQALAAQNGLRMSSTSRFIAWLSTEADAA
                     KCRMVNQGGSTCTPSGPFIWYNTNYEPVFTSFESATTGLLGSTLTMPSAAKYTEGRFP
                     APSMTDNVWTGTNIGGNIAGGFTCNNWNSPAAGTQGRTGTSDSQSSGWTSGATFGCDL
                     FKRIYCIAVP"
BASE COUNT         8919 a        11062 c        10781 g         9257 t
ORIGIN      
        1 caggtgagaa gatggtacgc gcgctgccac ctgcattcgt tttctgcggg ggagctgatc
       61 ctattctgga cgacaccctg cggctggccg cggcgctcaa gcgccgctcg gtgcgcacct
      121 cgttacgcat ctaccccgac aggcaccatg cgttccatgc atttgtacca ctcgaccccg
      181 aggcgaggcg ctgctggcgc gatcagatcg aatttcttga agagatcggg cgcgaatacg
      241 gtgcggcatg actatttccg gttaaagctc agtatcgcta tttgccgtgc tgcgtagccg
      301 acactccacg cctggtcttg aaccagtggc gaaaaaatgc cgctgccgag agccctcttc
      361 attatgaata gcttccagaa atcggccagg ttgatctggt cagctgcctt gattttcgag
      421 aaggcgcctg agatttgcca gtgcggtcgc tgattgcccc acgatgcggg ggcgtactac
      481 gagcggctcg aagtagcgca atggcccctt aacttcgtag gtaagggtgc gcacgaagcg
      541 tgtaccgcca tcgtgcgctt cgaggtcgta gtggatcgtc aaatcgaaca gggatgaatt
      601 gccctgcacc tcaaacgcat cggcgcggcg gcttaatgtc acgcggtagc gtgtaggccg
      661 ctgcactttg acgagcgggt aggtaaccgt gatgatttca tcaaattcgt cgccttcgtg
      721 cagcggcacg cgcggcaact tcgccgaggt cgatgccggg tgccactcgt gccacagatc
      781 aggccgggtg acaaagtcga atacaatgcg cggcttcacc gcgataaaaa tttcattcgt
      841 gagttggggc acggcacccc ttagttaatg cgcaaggcgc cgccaatcag aaattagccc
      901 cgacgcaatc ggggtctgag ggcgcagtct tgcacagcgg ccagggtgta gcaaccgccc
      961 ccgatgcatc aattgtgcct gaactcgtgg cacaactgcc gccggtattg ctaccgtcgc
     1021 aaatacccat ggcggtcagc ccactggttg ccccacccca atagttgttg gtaaaattta
     1081 atgtgccagc agccccgtag aacaagttgt tgccgtttgt acctgtgttg gaaacaaact
     1141 ggttatggtt gactgtcgcc gtcgtaccat tggagaagat ggcgccgccc cttgtagcgg
     1201 cattggttgt gaatagattg tataccaggg tattattgtt tcccagggaa accgcgccag
     1261 cgtaactatt tgttccggtg ctcgtgtttc ccgaaaaggt gtttcgcgtc agcgtgttgc
     1321 caaagccatt gacgaccaac gcgccgccgt acgagccggc tgtcacagct gttgctgtgt
     1381 tgccggtaaa aagattattg gtgatggtgt tgttgttcga catcaggtag gccgcagctc
     1441 cattggcagt gtttataagg gagaccaggt tgccctggaa agtattgccc gtaatagtat
     1501 taaagtcacc attaaaaaac agcgcgccgc cctggtggtt gctttgcgtc gagtttaatg
     1561 aattgttaat gaactgtgaa gaagtgacga ggttgttgtc gcctcccaga tacgccgcac
     1621 ctccccggag aaaattggcc gcgtgggtaa tggtattatt gaagaatttt gatgaataga
     1681 ttgctgctcg ctgcgtagcg tacagaccac caccgtagag attggtggca accgtgttat
     1741 tcaggacgag agtgtttttc aggacaacct gccgaccagc cgcctgattt gctgagatac
     1801 ctgcaccatt cgtcgtcgta gtacgattat tctgaatagt gcaactgtcg aaaaagagcg
     1861 actcgccggt cgtcacaccg ggccccgcga actcgacgat tgtatactgc agtaaactgc
     1921 ctccgctgta gttatcagaa gtaatcgtgc taccgatgga gccggaggca aagttcagac
     1981 cggcccaatc gccgggagct ggcgaggcat tcgccgacgt gaagacaatc ggagcagtca
     2041 gggtgccctg tgcaactaat gagccgccac ttgcaacatt tatggagaat gtagggttca
     2101 cgagtacgct tgtaccggcc gcgactgtca acgtacccgt tatggaaatg ttgccggtta
     2161 cgcagtaggg actctgtgcg ggtgtccacg ttgtggtgcc tacggtccct gaaaccgaag
     2221 tggcgcatcc cctcggcact gcagtcgttg tggcctggta ttgcgtcgtc gccagattgt
     2281 cctggactac cacccggtag ggataggtct gccccgccgt taagccggtc gtgtcggtat
     2341 aattcgtcgt cggtaagaag acggtactta agagctggta gccactgcca gaatcgcggt
     2401 acacgagcgc ctgggtggtg ccggtcggaa aatgccaggt caccctgata ctagtcgcag
     2461 attgcgcgaa agcggctaac gaaacgctgt caaaatagac ctggaaattg ttcgagaggc
     2521 cggtgatcga cgcgtttacg gtgtccactg cccttatctg caggttcgta taagattggg
     2581 caagtgtcac agaaacagtg gcaacaccgt tcacgaagcc cgtgaccgtg cttgggctga
     2641 tagtaccggc tacaagatac gtactgagat tcactgttcc actataggca gtatacgtcg
     2701 aattgtctga attcgcggcc gtcaccgtta gattgaacgg ctgcccttta aatactgcgc
     2761 cgggtgcact gatcttgaac tggttaaata cggcctgcgc agtggcagtt atattgttgc
     2821 tcaccgtcgt aagcattgaa tctttgggat cagtgaccct aagcaggata acttccgatg
     2881 cattagagac aagcgccgga ttattgtaga ccaccgtgaa attctgtgtg cccaaatacc
     2941 atccgccgcc gttaacgctg gcaatcgttc cgtttccaac ctgcagcgtg atatttaccg
     3001 ggttctggta gtcctgctgg ataccctgcg gcatggcagt gttcgttgcc gtcacggtca
     3061 cggtgaacgg ttggtcgaca gtgatcggcg agggcgaggt tatcttgagg ctcctggctc
     3121 cacccgtggg ccactggaga ttcagcaact ccgttgtcga atattctttg caggcggcac
     3181 tcaatgcaac ggcaaggagc agaatgatgc gacataaccc gttcatacag aaattacacc
     3241 tcgcacggtg cttgaggcgg agggtttagt ttcagaaagt caactttttc agaaacgtaa
     3301 ataccccctg ttcagggggc aggcgccttg cgcttcttca taatgaacag atgcccgctt
     3361 tctgaccaga aaacacccgc tgtcactaca gctgcaccca atataccctg cacatgagcg
     3421 gcctcgagcc cgagtaagat ggcatacacc atcgcgaaca ctggctccaa tgcaaatatc
     3481 actcccgcac gcgagggcgt tgtcagcggc tgaaaacgca tctgcagcag aacgacgata
     3541 acagtacccg gaattgccag gtaagcgagt gcgagccagg gttcatgacc cgctgcaaga
     3601 ttgctgttgt gcaattccat acccggtaac gacagcgcgg cgaccgtcag gcactgcagc
     3661 gtcgaaagca caccgcgggt ttcgggggtg ctccacgcgc tgacgccgat gatgtatgcg
     3721 ctgaaagcca gcgcacatcc gagggtgagc caatcgccac gattgagcgc cagatcgtcg
     3781 ccgggcgaag tcagcagaaa gagcccgagc agaacgacag cgattcccgt cacggtgtac
     3841 acagaggggg tccgtcggta aaaaacgaaa tacatcagcg gaataaagat caccagcatt
     3901 tcggtgatga aggccgaacg agagctgctg gtaaagacca ggccgaaggt ttgcaggctg
     3961 aagcccgcga agaagaacaa gccgagggcg acgccgcgca acatgaggcg aaagtccgcg
     4021 cgcacgacct ggcgatagta gatcgctgcg aacaacgatc ccgcgaccat gaagcgcagg
     4081 ctcacgacag tccagggcga aatctgctgc agcgcgatct tagctgcggg aaaagtgcca
     4141 ccccagataa ccgtgcagag cacgagcatc aggtcgggta gagcaaggtg gcgcacctgg
     4201 tttctgaaaa tgggcgttag cccgtcgtcg ccttgctgag ctcaacactc agcagggtac
     4261 tcacaccggg ttcgttcatc gtgacgccgt aaagcacctg cgctttgccc atcgtcttct
     4321 tgttgtgcgt gatcagaata aattgcgtat tcttcgcaaa gtcgtcgagc agcgtgagaa
     4381 accggccgac gttctgctca tcgagcggcg cgtcgatttc gtcgagtaca cagaacggcg
     4441 acgaacgcac catgtagatc gcgaacatca gcgcgattgc ggtcatcgcc ttctcgccac
     4501 ccgacattag ccgcatcgac ttggtttttt tacccggtgg agttgcctcg atttcgatac
     4561 cactggcgag cggacgttct ggttcgagaa ggtctaactt cgcctgaccg ccgttgaaca
     4621 gcttggcaaa aatctcggcg aagttatggt taatctgctc gaacgattcg cggaacagcg
     4681 cttcagactt gccctggatc tcggccatga ccttcttgat gtcttcctgt gccttgagaa
     4741 tatcgtcgac ctgcgcctgc tggtggtcgt acatctcttt gatatggcgg tgttcttcga
     4801 tcgccagctg gttaatctgg ccgagagccg aaatgccgcg ttggatttcg gtcagcttct
     4861 ctttttcgtc ggccaggatc agcttacccg aggtgaactg gcccgaaagc tcctgccagg
     4921 tgagtgaata gtcattgtag atattctgca gcagggtatc gcgggtacca ccgagcgcgc
     4981 cgatcttgcc ctcgagttcg ttgatcgcaa caaacacctc gtcggtcttc ccgctttcgc
     5041 tcttcatggt cagcacgagc tcgcggcgtt tgtcgtcggc cttcttgagg tcgcgctcaa
     5101 tgcccgcaat gcgctgcgtt tctttctcga tgctcgattt gagcttttcg acttcgcgcg
     5161 tgaggttctt ctgctcctgc gaaagctgca cgagcgagcg gtcgatttcg gtgaattgcg
     5221 agcgcacgaa acccatgttg gtttcttcgt tcacaatctg ctgcgcgagg ttcttctctt
     5281 tctctttgac gctggtaatc tgtacgttga gcgacttgat gtcaccattg aggcgttcgc
     5341 gttcctgccg gctcgcggca gactcttctg ccagctgctg cttgcgtgcc agcaggcgct
     5401 gcgtctcgcg gtttgccgca gcgatacgct gctcgagcgc agcgatctgc tcgtcgagct
     5461 cttctttgcg cgaatgcaca ccgcctttct cgaacaggat ttcatagaga ccgcggctca
     5521 tttcagccag cagatgcgcc ttttccgaaa ttttgccgaa taccttgctg tctttcaggc
     5581 gctgcaggtc tttacgcatc tgcggcaggt ggtcatcggg cgaagccagc accgcggtga
     5641 gggttgcctc ggtttcgttg cagtagagtt cgatctcggt gaaggcctgc tcgcggttct
     5701 tcgctgtttt ttcccagcgc gatttttcgg tcttgagcga gagcagcagt tcgcggatag
     5761 tctcttcgta cctgccctgc acttcggaga gcgatttttc cgctgccgat ttatccgctt
     5821 cgagtttctt cagctcttcg tcggctgtcg cagattctgc tgcgcattct tcgatgcgtt
     5881 tctgcaggcg agacatctct ttttcgatat ctgagacctt gaggtcgagc tgcagggtga
     5941 actggttctg ttcggcagct tctttcttca gttctttgat gcgtttttcg aaacgaccaa
     6001 tctgttcttt gagttcggca agctgggcag tcagcgaggt cttgcgttct tctttctcac
     6061 tgattgcctg gttgtgccgt gcaatcgttt cgttcgccac ctggctggtg acgtctttct
     6121 tgtgtaattc ttctttaaga ctgcgttcct cttcgtcgag gccggtcagg cgctcctgca
     6181 tctggatcag cttctgctgg atcttttctt tctcagcatg gcggcgcgcg agtttttctt
     6241 tcaggtcttc gagcttgtcg ttcgcatcgc gcaccatcag gtagcggatt ttgaggtcgt
     6301 gctctttgta ctttgccatg agcgcattgt actcggcggt ttttttcgcc tgcgcttctt
     6361 tgatcttcag ctcgcgttcg agctcccgct gtatatcctg tatgcgggtc aggttgagct
     6421 gcgtgttttc gagatttttc tctgcctctt cgcgctgcgc cttgaagcgc gagatgccgg
     6481 cagcctcttc gaagatgagg cggcggtctt caggcttact ggacaagatc tggtccatgc
     6541 gaccctgttc catgaacgag tagctcgact tgccaatacc ggtgtcgcgg aagaggtctt
     6601 caatttcttt cagcgccacg cggctgtcgt tgataaaata ctggctctgc ccgtcgcggt
     6661 acagccggcg accgaccttg acgtcgtcgg tgtcgagctt gagcaaccgc atgcggttat
     6721 caaaggtcaa tgagacctgc gcgaagttgg cagccttgcg gctcgacgtg ccggcgaaga
     6781 tgacgtcttc catcttttcg ccgcgcatgg tcttgaccga cttttcgccg agcacccatt
     6841 tcatgctgtc gagcacgttc gacttaccgc aaccattggg accgacaacg cccgacaccg
     6901 aagtggaaaa cgtgatcttc gtcttgtcgg caaacgactt aaaaccaaaa atttcgagct
     6961 ctttgagggt catggtacca ggttttctac ggttacattc gcgcttgctt cgctgtaggg
     7021 aaaaaaatat tcatcactga atggccacgg gcgaacttac aggaaagtat gcccactacc
     7081 acttcggcta tgaagaaggc cgtttttcta ttttcgccga caccgaaaag ctaaaaggaa
     7141 tttcgctcgt ttcgacccgc gacccggccc gcgaagccga acgccacatt ggcgcttttc
     7201 ggctcaaacc cggtatgctg gccgtcgtgg cgggggttgc gcaccttccc atgctcgaaa
     7261 tgctcgccga gcagctgcgc tcgcccgaag gcaaaggggg agttatcctt gctttcgagg
     7321 ctgacaccga actttacctc tctctgcgca aggcccggcc cgatattttt gccgacgtag
     7381 cggtcattgt acccgaaaca ctttcggtgc taaccgaact ggtggaaaaa atcgacgtcg
     7441 agaacttcgt cggttaccgc gtcttcgaga acaggccgag tatcgattta gctcccccat
     7501 tctactcggg tgccgtgacc gacttcaaga aaaagctcgc gtcacgcttt tctgacctgt
     7561 tcacgcggct cgagttcgaa gaacgctggg tgttcaacag cctgagcaag ctgccctcgc
     7621 tgtcgtcgat gcgaccggtc tcggcgctgt ttggcctggg tgccggggca aaggcactga
     7681 tcgtgtcgac agggccttcg ctgcgcgatt cgctcgccga aattgccgcg aaccgagaga
     7741 ggtattttat cgcgtgtgcc gattctgcct accgcgtgct ggtgcgctac ggcatcacac
     7801 cgcacctggt cttttcactc gatagccagg cgcacaccgc gaaacacttt ggccttctgc
     7861 cccgcggcaa aaaaggcgcg tttcctattt tggtctgcga cgcggtatct aacccggtag
     7921 tcgcgcgtga ctggcgcggc gagatctgcc tgtcttttac ggcccagtac cccgatgacc
     7981 ggcgcgtcgt gagcccgggc tgcgactatc tcgaagaagg acaatttttc gccggcacgc
     8041 cggggcttat tcctggcgac ctgcaatcgg gaggttcggt cgcgacatcg attttcgacc
     8101 tgatgcgcct gatggagttc gacgaaatta ttctagcggg acaagacctt gcctatacac
     8161 gccgcgaaat acacacaccc ggcacgcacc acaccgatga gtggctcagc aagacgacta
     8221 accgcctgca gccgctcgaa aatatcaacg agcgtgtgct gcgtcgccgg cataccaaac
     8281 gtgaagtttc actcactggt cgcccgatcg taacggacta tattctttcg ctttaccgcg
     8341 actggttcgt gaatgcgatg caggcagtac ccggcaaaac agtcaatgcg acgcgcgatg
     8401 gactgcagat tccgggagca aaacagaaag agacgatcat gacagaaact gccggtaaaa
     8461 accaggagat attacagaag ctgctggctg cgccgccgat ggtaacttat ccgcaacgcg
     8521 tcacggcatt gaaagacaag atccagggtg ccaaccgcga gaacccggag cgggatctcc
     8581 ctttcctgcg ctatgtcggg cgaaagtatt ccatcaaggc gctccgctca cccgagcagg
     8641 caagacgact caaagaaaag cagctcgtta agcagcgtaa ttttctgcgc aagatggctt
     8701 cgaagctaca gctttcgccg gcagagactg gagtcgtccg ctaagatgcg cccgcgcttt
     8761 tccaccatct tctggccttt ctggctctac cacgtgagaa gacatgcctg gctgatgctg
     8821 gtccttttgt ttttgaccag ctttatcggc ttccgttatg gcaggcagat tcgtattgat
     8881 acaaacctgc tgaatcttct cccctccaat accgaatcgc tggtgcggct gacgcagctg
     8941 cgtgacgcca gcgacgtaaa aggttttctc gtcgccactt ttgaatccga aacggcgcgc
     9001 agcgaacgcg atctcactgc gatggcagaa cgtctcgagg caacgatcgc ctcgcaggat
     9061 ttcctgagaa gatccacgag cagggttatc tacggtattt caggcgagtt cttgctgcgc
     9121 tattctctct ggtacgcgga ctacggcgac ctggtgaaag tgcgcgaccg tctcgacaac
     9181 agcatcaagc aggcaaaacg acgcgagaat ccctactttg aggacctcga agagacggca
     9241 cctgcgccgt tttatatcgg tgatgtgctc aacaagtacc gcaagaaata cctgagtagc
     9301 ggtgcgttca tcgacaacga ccgcaagcgc atcgccgtcc tgctgtcgct cagggagccg
     9361 cccgacaaca tacagtttgc tcaaagttac cttgcggcgc tcaaagacag catccgcttc
     9421 gaggcagaga agcgacaatt ccgtcttcac ctttccggcc ggtacgcctc tgacgtagaa
     9481 aaacagcgcc gccttgccga agacattagc cagtcaacga caatcacgtt actgatcctg
     9541 atcgcctcgc tcgtgatctt ttttcgctcg ctgcgggtgc tactggtcgt cggcttaccg
     9601 gtcatggccg cgctcggcta cacgtactgg gccgcgtggt attttatcgg gcagatcagt
     9661 atcatcagtt cgttcctgac gtcaatcttg ctaggtctgg gcatcgacta cggcattcac
     9721 ctgtttgccc gctacaaggg cgaacgcgtc aagggccacg gtatgaccga gtcgctcgaa
     9781 atcacgatgc gcaatctctt tcggggactc agcttcggca tgatcagcac ggcggcggtg
     9841 ttcctgaccc tcagtttctc gcgcttcatc gcctttgccg agtttggccg cattgcgctg
     9901 gggggaattc tgtttttttt ctcggccttc gtcctttact ttccggcttt agtttttctg
     9961 acagaaaagt attcattcgg tactaccgaa gcctcgcaca tactcgaaaa aacccggcgc
    10021 attcgcggct ggcagatggc catcatcctt tttattgccg tctatggggc gataactgcc
    10081 ttacgtccgc catttgagta cgattttttc gagctcgagt cgacagacca ccgccgcacg
    10141 aaaaaagacg ggcagcacgg gcaaatacgt atcattgaaa accgcgaaca catggtaatc
    10201 tacgagctgc cgaacctcga agcactgcgc gcgacggaac aaaacctgcg gacgctgaca
    10261 gcaggctcag gactggagct gcacagtgcg ctcgatctcg tgccgaccga ggtgactcgc
    10321 aagcaggcgg tcatcaggca aatcaatgca ctgctgaagc aggccgaact ctatgccctg
    10381 gtatcgctcg aaggcgctac actcacccgg attcgccagg ggttgcgcat gacagaaggc
    10441 cggcccgtga ccctcgaaga tattcccgaa gcgttcaggc agcatttgat acagaacggc
    10501 aaatactacc tctatgcatt cccgcgcgaa gtatctgacc tccgccgcgg cgcgcttgat
    10561 ttcgcggcaa aggttcgcga gacctgcagc gtcgtgctca gcgtcggggc aacctgccca
    10621 cctgaaaaag tggtacgcgg catatcagat ctttacgtgc tcgatgacat tctcaatacc
    10681 gtgatgcgcg attttgcgaa gcagattgca ttaatcgccg cggtcattgc ggtcatcgtg
    10741 ctctcgatga cgagacggct cgcaggtgtg acgctgattc tggcaccgct cgcggcgggc
    10801 atacttggtc tcttcgcgct catcggcgct ttccactacc tgtctgccac ctggctgttc
    10861 gagctcaact atatcaacct gctcgccatt ccgattctgc tgggcgttgg catcgacaat
    10921 ggcatctacc tctactctca cgcgcgcgaa tcgggactct cgcaggtgaa ccagatcatg
    10981 gcgagtaccg gcggttcaat cttcatttcc aattttacca cctcaatggg atttctctct
    11041 ctgacgattt cgagccaccg cggacttgct tcctttggtt ttctgacatt cgccggtatg
    11101 ctctgtatct tttatgcgta ccggctgctt ttcccggcgc tggcgcgctg gctgcaacag
    11161 gcagaggcag agatttgatt cgccccctgc tcccattact tcttagctcc ctctgctggg
    11221 cgcagacgat tgatctcaag agcgaaaagg aatcactggc aaaaatcgat gccgctgcaa
    11281 aagccggtga cgatcgtgcg ctggtcactg aatgcgatcg cctgctcgca gttcaccagg
    11341 tgacagccct ggcatggtac acgtgcggca agcacctgct gtttgtcaaa accacggacg
    11401 aaaagcaatc gctcgccaat gcgaagacgg cctacggtcg gttagcacgg gcggtcgccg
    11461 atttttcgcg cacaggtaaa cagacgttct atgctctcga tgccctgcag taccagggac
    11521 tggccgcgct ggtattgcat gacttcgacc gcgcgatggt acactttcgc gctgctctcg
    11581 cgcgtgataa ccgtattgct gcaacgtggt acaatctagg ggtactctac gaaatgcgcg
    11641 ggcagcagga agaggccatg cgctcgtacg atcgttacct gaggctcaaa ggtacgggcg
    11701 actcgcagga attttgagct aatttgtgca caccctgtat acttcttgac aaagtgttac
    11761 aaaatgtaac aattgtctat gaaggtactg agaaggcaga aaaacctgag tttcggtacc
    11821 agccccgaca atgagcaggg cctgaagctc gaccttcgtt ttgacgaaga caccaagacc
    11881 gcctacggtg agatcgttct ccccatccat ttccaaggca accagcccga atgcgtccac
    11941 cccggtgtaa tcgccgtcat gctcgacgaa gtcatgatga agatcaacca ggcgctcaac
    12001 ctgaacgtca ataccggcga aattacgatc cgttacctgc agctggcaaa agtcaacgag
    12061 ccgctctacc tacgcggtta tttcgtgaaa aagaacaaac gcgtgatcga aaaccgcgcc
    12121 gaactcgaag acgacatcgg caaaatcgtc gcccgcggta agggcaagta tattgagcaa
    12181 gacctctaat ctttcaaaag taatgacgcg ctgagcgcgt cattactttt gagcacgcgc
    12241 gccagcgcac agttaagccg gagtggtgaa atggtagaca cgccgcactc aaaatgcggt
    12301 gggggcaacc ccatgtcggt tcaagtccga ccttcggtac actttttatc atgattgcgg
    12361 cataagccgc aatcatgata cctaagccgc atcgagcatc gacatactca gcaccacatc
    12421 agaataatcc cgtttggtaa tcagctggtt ggcgtcgcgc tcagccaggc gtttgaattc
    12481 tgcgaggtta tggtggctaa gggcaaagat ttcacggcgc agtgatgccg cctctgccgt
    12541 tttacccgaa gctgccgccg cgcgcgcggc ccagagcagt gcctggcctt tgcggtacgg
    12601 acccttttcc agcgccacgg cttgcatcag gtgacggtac gcacgttcgg tgtcccgggc
    12661 ttccaaagcg aaaactccct caacaaattt cagtgagcca tcttcgacag cgtatgccga
    12721 ggctttactc aggtcagcac gtacctcgtc gagcgagcca cagttgaagt cggtcaggta
    12781 agcattttgg aaacgccggt atgcctcgag cgccgggccc gaaccgtaac tcgcagtcac
    12841 agcgctgagt tgctctttcg ccgggtcgac gacgcggagc gcatcacccg accagttgat
    12901 ttcatggttg atgtaggggc cccagcctgt cggcgcttca ccgacagaaa gtgaaatgag
    12961 ccctgactgc agcttgaaaa cgactgactg caccgacatc acatagccga tcaacgagcc
    13021 catcatgcgc gggctaccca gtgcatcgac ttcatggtcg gcaccgagaa tatgctgcat
    13081 gtcggtgagt gttgcgccac ctttcgcctg catctcggca aaaaatttgt cgagcaggcg
    13141 caggcgatcg acggtgtacg aagtccagcc gttgcttgtc gcaatctcgc cctgcaccat
    13201 gtcagggtgc tggtagtggt tcgtgttacc gaggtaacca tcgcgtgccc aggtgacccg
    13261 catttttttt gcggtggttt cgatgatcgc ggcattcttt tctttggcgg aggtaaccac
    13321 gataccccag gtcgaggcga ccttgtggcg gctcacgatg tcgatcgcgt cattgatcgt
    13381 gcgcgcctgg ctgataattt cgtggccgag gtcgatgacg ccggtcgcgg caaaatcaac
    13441 gtcgcggtga aaacgggtgt gaacagtcac cgtgatacct tcggcgttga aagccgtgat
    13501 acccggaaca tccgcgccgc gcgcgccgag gtaaccgtac ggaataccat ggtcggggct
    13561 gcagtagaca attgtcggcg atttatccca gactgcgaca ccgggaaagt caaagttgcg
    13621 cgcgtgcatc agctcgccgt cggcagagtg cgagttgaac acaactgccg acgtgcaggc
    13681 gccaagggca tgcaccagcg gtttgtcaaa aacatcaagc gctccgaggc gccccagcac
    13741 accgatcgag ttctggaata cgtccattac cagcatatgc cgcgccatgt cgggcgacat
    13801 gttcgcggcg cgcgccatcg cgtgcgcgcg ttcggtaaac tgcggcaggc gattcttcac
    13861 catcgagttc actgcccagt tcatgagctg tgccgccacg cggcgtaccg gccctttgcg
    13921 catgcggcgc ggcatgcctg aaaccaaaag cgactcagcc atgcgcgggt agaaatcaag
    13981 cagcggctca taaccgccct gcgagccaac gatttcgccg tattgagctc ccatctgtgc
    14041 ctgggagccc ttgaggcgaa tttcaagtaa cggattggtg ataatgcgat cgggtatcat
    14101 ggtacgaccc cgtgatcttg tgtgcgccgc gtcaaatcaa aaaatcagcg tcaggctgaa
    14161 gaggtgcgca tagcgccagg cctgtccgtc tgtaccgtca aaattcagct gcgggtaagc
    14221 ggcgccgggt ttgaatatcc cgaaacccca aaaaaactgc aggtctgcca tcgggttgaa
    14281 tgtaagagcg agatcgccgg catgaccaac atcattgctc gcctgggtcg ctgcggggtc
    14341 tgaaatacca cctgtggcaa cgctcttgcg gtagtagctg tatttcagct ggaccgcgag
    14401 gttgcgcagc gtgaagttcg gcttcagcgg gcgaaagtag gtaccgagcc tgacgatctg
    14461 catattggcg aggcggggct tgagcgcaag accgccggaa tagctgccaa agttatggaa
    14521 tgccgtgtaa gacccttcgg tattcgactg cgaagcgttg gtcatgcgcg agctatcggc
    14581 atcgcccgag cctgcggcaa cttcagcggt cagctgcgaa tcgtaatcgc gcagcacggg
    14641 caggttgtac gtgacattcg ccagcgctgc cattgcagaa attgtcgccg cctctgaaac
    14701 gccgttcagc acgaggttgt acgtgcttcc accctggtag atgatatcgg cgcggtagcg
    14761 caggttggaa ttcacgatgt agcccctgag acccagcccg ccgtaccaag gatgatacgc
    14821 gtacttggtg cgtgatgtat cttcgctgat catgtcgcgc gaatacaacg catatgcggt
    14881 agcctgcgat cccgcgtatt cgggcgtgaa atattcggcg gcgaagaaca cgcgcctgcc
    14941 cgagttttgt attcccgaat cgggtaagag gttgggggta ttcacgaatg gcccggcatc
    15001 gccgttgcag ccagtcacgt tcagcgcgca tgctgaagta ccgtaatcgg ctgaataggt
    15061 agcaaagccg cgcaactggc caaagggaaa ccggccattg taaccaaagc cgtctgcgaa
    15121 atttgcaaac aacagcccct gctcgatcat gtagaagctg cggccggctg tgaagacatg
    15181 cctgtcgtac ttcgcctcgg ccgcgagcag ctcgatgctg ccagtgactt ttgtctgctg
    15241 cagcgcgcca accggttcga cgtagtgctt caccgtgaga agcccgcgcg ctacaccgct
    15301 gtacttatcg ttgtacgagt acttcgcaaa gggcctgtag aacccggcaa acaaaccagg
    15361 gttaatcacg gggtcgatgt aatacgtgtt cgttgccatc aaaccgccgc gcaggtagtg
    15421 gtggttttgc ggttcttcgg cttcaaccgc tggcggtgtc accagcacag gcggtggagc
    15481 gattttcgtt tcggcctcgg ctgtagactt cgcatccgct gcgtcttctt ctgctgcttc
    15541 cacacgtgtt gccgcgggct tctccggttt ctgtggcgcc ggctggggtt tggctgcagt
    15601 tggcttcttc gctcgcttct gctgcgcggc aagtggagcc accgaatagg caagtatcag
    15661 agcgagtagg tacaacagtg atctggaatt cataatggat taccttgcct ctgcaggtgc
    15721 gggggttggc ctggtcccat cagatttttc ttcgcccttc gcttctggtt cgagtggctt
    15781 ggttttctgg tccatttccg ggtacgtctc ataaccgaag tctcggtagg ggttcttggg
    15841 ctgcatccac gaagcttttt cgccggcgcg ctgcatcgcg atatccatga tcgtaacgag
    15901 ctcttcgccg ctgagttcat cccaactatt cgaacctttg tcgatcagcg agagcacttc
    15961 ggcattttgg attgcgtacc attcgctgcc cgttagccga aagagcaggg tcttctccag
    16021 gtcatagaga ttcagcacgg ctttggccac ggcacctttt gtggcgggct tcaggtgtat
    16081 gcgccccacg gctgccgtat cgtatatacc gagctccgca agccgggcac agcgagcctc
    16141 atcggtcgtg aacttctcga gctcattgcg gtgtatcagc acgagcttgc acacatcgga
    16201 aaaggttgct ttctttgtga tggtaatggc ccgcagcgcc gagagctctt catcgagaat
    16261 gaggttctcg gtgaggggtt gggctgcaag cgctgagatg aggataaaca gtgacagggt
    16321 ctgcctgaat agagatttca ttgacttgat tcctttaaca accagaagtc gtcgcactta
    16381 cctgcgttgt acccgtactg atatttcctg caaggtcacg ggcccgaatt cggtaatagt
    16441 atgtcgtaca acctgttaat ccgtttgaat aatatgatgt tgcacctgca gacgacgtaa
    16501 atgttgccgt gagcgtactg cagttaccgc tggtggtcgt ctggcaaatt tcgtagacca
    16561 gctgtgctgt tgatgttccc gcatcgctcg aggcgctcca catgatctgt acctgcgatg
    16621 tgctcgttgc gtcggcaaaa ggcgaaccgg gagtcgtcgg cggcatggca tcggatacgt
    16681 gggtggcgtt tacgactgcc gagtttgcca tccccaattt acaggcaagc gcgcggaagt
    16741 tcctggtcag gttatagaag ctcacagccc ccgaatatag ggtgcccgtt gtacatgcgg
    16801 gcgtagcgct acacgcaggg tcggtcgccc cgtcagttgt gtagcatata atggcgcccg
    16861 aagttgcgga gctcatggtc acgttgagcg gcattgcgta atgaccggct gccggcgaga
    16921 atgtcggaat tgcagcagta ccaccgccgc caccctgggt tgcaggcgct gccggtggct
    16981 tggggttaat ctgctgccac atttccctta ccgtaatacc atcgacgaca tacatgtctt
    17041 cgctcgtgcc gcccgattcg gtacctgaaa taaacatgag agcgccgttc gaaccaccgc
    17101 ccaggccacc catgaccgtg acgttgcgcg cggtgatgcg ccctttggct atcgcattat
    17161 tcacggaagc gaggttgcca ccgttgccgt agagcgacat gctcgtgatc gtgccgtcga
    17221 tcaatgagaa gaaaccgtcg gtgcgcgagg tgaaagtgcc ttcgacactc gcaaccgagg
    17281 tagcagtctg ccccgtggcc atcgccacca gcgcatcgcc cgccgatgcg tggttcactg
    17341 catagaccgt gcccccctgc cgcgcgtaaa cccggttgcc tgtatattcg acgcccagca
    17401 ccgtcgaagt cagacccgcc acgttcgcaa tattggttag ggtcgtgtta tacggtccgg
    17461 tgggtggaat agccccggtc tgctgcacat tcccgccagt atttgtcaga aatatgcgca
    17521 cgttattgcc agtagccgtc gcggcatatt gtatgcggtt ggcgtagttg agcggtgtac
    17581 caccaatcgt gagtacagtg ggattttcaa atgccgggaa aactgcatcg ttgttgtagg
    17641 cattcaccga gattgccata aagagattat gccctgtgca accaccgcat gcccggtttg
    17701 aaccgaagac cagcacaagg tagttgtcac ccatctgtat gacgtagggg ttgtactcgt
    17761 cactcgacgc ctgcgaaatc gcggcaccgc cattcagctg aaactttgca gcgccgttca
    17821 cccgggcttc gggaagatct tttttcgcac agccaatagt tgccagtaaa gtgaggattg
    17881 ccccaccaca cgcgacggct ttcgtgaact tcattgatcc ccctaaaact tctgcagtgt
    17941 gggctcgcct actgaagatg gttgggtgac tgagttgata gaattgtaga gtccctggat
    18001 atcgttgcca aaacctgatt gtatgtcggt aatattgccg agaaacgatt gctcaggcgc
    18061 agatgagcct gacgagaagc tgcgctcggc acgcgagtcg aaagtgcgcg tcgaataagg
    18121 cccctgcagg tcaccgctta cccgtgccgc gtcgccctgt ggtactgaag caaactcgag
    18181 gttgttaaag aacagttcgt cgtcttcatc ggcctgctct ttcttccggt tcgtctgttt
    18241 cagcgctacc atgtaggctt cgagctctgc cttgtatgct gcttctgccc agagcagttg
    18301 cagcctggtg agtgcgcgta cctgcatcag agcctgtata atgtctttgc ggttggcctg
    18361 gttcgtctca agtactttct cttgccttac cagaagttcg cgtgtcagtg tgcggtggtc
    18421 tttgatttct ttgaccgcct gcacgaattc gggcgcatcg gtggccgact gctgcagggc
    18481 aatgaatgcc tgcttgtcgt cgtcactcgc cgccttttct gccagcagcg ccgcccggtt
    18541 cttctcgtcg cggtcgatga ggtaggcacg ctcgagcgct gcgaaggctt tctgcagacg
    18601 ctcgcggttt gtcgtggccg tttgtttatt gcgtacgaac gccgcggatt caattgcctt
    18661 cacctcgtgc tgcgtgccgt gccgctgcag atcgagaaat gaacccctga caccgacaac
    18721 caccgtgggt gttttcacca aaaaagtatt cttctgcgcg ccggcaggct tcttgacata
    18781 cgcgcccagg ccgccttgct gtataaagag ctcggtcctg ggctttgaga ggtcagtcgc
    18841 aagctcgttg aagctgagca cggtattttc tttcagcgcg atctggctgc cctcgggcaa
    18901 ggccacgact gccgcactgc gataacccac ctgcacgctg ttttcttctg agagtcggct
    18961 gtcgattgcg gctttaaccc agcggcctgc ctcgaaaacc tgcaccttgc ccttcagcgt
    19021 caagaagcgc gcctgcggcg acttatccac cgccatgacg gacactggaa tgaaggcaaa
    19081 caaaagcagc acggagatgt aggtactcat acgtgtaccc gctgtctgcc cgggtatata
    19141 gcccgcgtaa agtgtatttc tcacaggtag acagccagcg aaagaccacc catcttcttc
    19201 tgcacggcgt cagaagtact cgcgcagcaa atcgttcgct tcttgcctca cgataatact
    19261 ctggctgcgg gtcagggttt tcagcctgac ctgcgcttca cggtttttgg tacgggcgag
    19321 ctcgcgcatg accagtataa gttcagaggc cgacttgcag tggtagctcc gcttgatcat
    19381 gtaaggccag gccgattcat cgtcatacca gacaagcgtg cggtaaattt cggcaaccac
    19441 agagggattt ttctcgttca ggcgaaagag cactgtataa aagtaagtcc gctttttacg
    19501 ttcgctgctg aaggtgcgga gcacgcggta ggcggtctcg cgcacaagcc actctttatc
    19561 gcccgtgagt ctggctacct gcggcagcac cgcttcatta ccatggagct tttcgatcag
    19621 cagcagcgcc cgccagcgta taatggcgtc attattcagc agaagttctt cggcgcgcgg
    19681 caggtgctct ttgagtgaaa gggcgtgcga aacttcgagt gccttgacaa tgaccccggt
    19741 gtcgtcgtca gtcagggcat aagcgacgat acgatcgtca tcgccgctag taccccggct
    19801 ggcgacctgt tcaaggtatt ccacctggcg ctgccagtcg agcaggacaa attcttcgag
    19861 cgttagccgg cggttctgct cgccgttggt ggacggagca gaacggcagc ccccggagaa
    19921 gacaacacag agggcgatga agccattcgc gagcaatatt ttcactgcag tacaaacatc
    19981 ctttgcagaa ccagcctgcg ttcgtcgcca tactgcatcg agttagacgt catattcagg
    20041 tgaaccaccc cgtaggcgat cgtcgacccg acgattgcac cagtgagcac gtcggtaaaa
    20101 tagtgtttat ctgctgcgat acgcaggtac cccggcagca tcgcgacaag gagcgcaacc
    20161 gaatagatga gcgttgaata ccttgggtag ctttctgcca gcagcatcgc gctcgaaagc
    20221 gacaaagcaa acgaatacga ggtgtgcccg ctgaaaaacg aaagcttgct gtccgggttt
    20281 gaatagtcgt ttccgtagta cgcgtaaggc cgcgcacgcg cgaacgagaa cttggccatc
    20341 tgggtgacta cccccgtcat gacagcggcc tcggcgattg tgagcgcagc acgcgaataa
    20401 tcatggttgg tcgcgagcgg cagcaggaag gcagtcgtcg gcataacgcc gtagagcatc
    20461 acatcactca gcgtgctggc aaggcgggtg ttcgaccaga ccaggccact gcgaaccgag
    20521 gcatcgattg cgggcggagt tttccagcgc gcttcgtcgg gaaccaggtc tttcttgtag
    20581 agttcggtga caccgatacc gagagcgccg gttgcgacac cggctatctc ccacggggcg
    20641 atcgccgccc tcgcctgcgg agcacccgca agacagagtc ccgctgctat aatgccgatg
    20701 agtctgcgca taacctaaat ttctctgtcc gctctgaatc ccaaatgtag tttttcccca
    20761 aaggggctgc gcggcaggtt gcagacaagt acgccgaccg gtaaagcaaa acgtagcaga
    20821 ccagaaacta aaccttatgt caatacaaac tccccgcttc atcgacagcc tagaacgtcg
    20881 tttccacaaa ttcggcattg agaacctggg gctgatcctc gtcgtcatgc aggctttcgg
    20941 ctttttagcc taccagattt cgccggcggc gaaagcgaag ttcatgctga tacccgagtt
    21001 gctgctgcgc ggcgagatct ggcgggccgt cacgtttatt gcgctgccgc tgagcaacga
    21061 cttctggatc attatcgttc tcttctttat atactcgatc atgcagatta ttgaacaggc
    21121 gtggggcccg ttcaagacga ctctgtactt tctcatcggg ttgggcctgt cggtcgccta
    21181 ctcggtcgcc accggtctcg cgatcgacac cttcatgccg ctcgaaatgt cgctcttctt
    21241 tgccgtagca gcactctacc ccacgaccga ggtactgctg ttctttttta ttcctgtacc
    21301 catgtggatt cttgcagctt tccaggcgct gatcgtcgtc tacctgttca tcatcagcga
    21361 ttggatacag cgtggctact acgcggtagt ctacatgaat tacttcctgt tctttggcct
    21421 gcaccatttc ggccagctga aaacctggct gcgccgcagg cagtggaggt aagcgccaac
    21481 catgcgcaga ctgacaaatt ttaccgcggt tctgctgctg gcatgcggct ctgtcgcctg
    21541 gggtgcgggc aaaacctact ggtacgaaat cgatctgaac aggtcacaga tcgccttcct
    21601 ggcaaagtcg cgcattgcca aggcacaggg gctgttccgc aagtgggagt tcaagggtaa
    21661 gatttcctcg aactttcacg tggtaggcga tctcgccatt gactgcgctt ctgtcgacac
    21721 tgacaacgaa cgccgcgaca accacctgcg cgcagctgac ttctttgact gcgcacagtt
    21781 tacccgccat accttccgga tcctctcggt gaagcctgac gataaaacac cggccaaggc
    21841 ccgccggttc gacgtgtcgg gcgagctgac aattcacggc aaaacccagc ctgtttcgtt
    21901 cgaactccgc cgtgaaggcg atgagaaaag tttcgtgctc acgggcagta ctttcatcga
    21961 tcgcgaagca tacggcattg cctacaacag cgctttgaac ccgatagaaa agatgatacg
    22021 catcgacatt cgtctcgccc tcgtccgcaa gggtgaccgc ccctgatggc aaaaaaactg
    22081 cgttacaaag gcctgcgcat taccgtcagc ctggttattg tcctgggtgt catcgcattc
    22141 tgggtactgc gtcctctgac agaaacgccg atgaacacgg gggacttcac cccgatacgc
    22201 gacgactcac tggcggttaa attcgctccg cgttttgtgc cgcatgcgct ctatggtgcg
    22261 ccgacacgcc tgctctaccg catggcgcgc ggccgcgacg gcaatattca catcgcctac
    22321 cacccattct acccgcatga agaaaatccg cacgaaggca gtggggcagc gctcagccgt
    22381 ttcatctata ccggtggcct gcatattaag gacatcatgt tcggcccggc cgatatcgag
    22441 ctggttgaag ttatcctcga caagaacctc gtgcctgttg aactcgggta tgaagatgcg
    22501 ggtgattaca atccgaaagc tttctcggtg cgccacgtag tcaagctggt gaaaaaaccg
    22561 caggctccgt tctgcttcac aaccagctca tggaaccaca tgttcgcact ggccaggggg
    22621 gagctttgtc gttctgtgca ggggcccgcg ccggagtact ttaccgaggc cggctggcag
    22681 aaataccgaa tggtgaaaga aaccgaagcg atgctgcgcc gtaaccgcat gcaccgcgtt
    22741 tatgaacgtc ttgccgcgcc gtgatgctgc caggtcttac tggacccgaa tcgcctgaat
    22801 cgcttcctgc gccgcgctct tgacgtcttc atcgtagcgc gactgcagca gtttcaacag
    22861 tgggataaaa gacttccgga ctttcatgac accgagcgcc tggcagatct gccgcagcgc
    22921 acgctcatcg gccgtgctgt cctggaactc agtgaggtat ttgtcgagtc gtacgagcag
    22981 cgccggcaca atcactgctg cctctttcgg gtactgccgc aggctgcgcg cgcaggctgc
    23041 aatcacctga ggatgagtct cgtcgcgcag cgtcttgacc gccgaggcca caatctgctg
    23101 cgctaccgac gattcagaaa acacgatcag cgcagtgcag gcagcttcac gcacatcagg
    23161 gcttttgttt tgcaaccccg cgctgacgcc atcgagatag cgggtcgcta ccgactcgcc
    23221 ttctttgccg gactgccggt aaatgacgga catcttctcg agcgcacgca ccttcgcata
    23281 gcgcagtgtt tcaggatctt tgacaatctt taccagcagg tcgctgtggc gcatgtcgta
    23341 atcacggatg cggtcgagta tttccggccg cgaatcggcc atgactgcgg caacaggcag
    23401 gcaggtcaga agcgcgagta aaaatctcat gggtagttgt ttacccctgt ccattctctg
    23461 gccggcggtc gttatcgtgg cggtgctcgt gccggtggat ggcatggtta atcagccgcg
    23521 cgacatcgtc gagattgtcg cccttgcgca gcttgatgtc gttaaattcg ccggttgcca
    23581 ggtagtggtt caggtgcttc ttgatcttga agattggccc ggcaactttg tgtgaaaaga
    23641 atacgctgta gaccatgata atcagaatat tcaggaaggt cacgacgacc acgggccaga
    23701 ggtacaggtc aaaggcatta tagaagcgaa aaccgccttt ggtcactttc aagggaaagt
    23761 actcttcgcc ttcaccttca ggaacaatct gccagttacc gtctttgtct tttttcagtg
    23821 atttatgatt atcgacatcc atttgcgcga gcacagaggc gccatcgggc aaaagtgtat
    23881 aggccttgtt cttcaacgca aacagcgcac caaggacaat gactgccgat atggctacta
    23941 caatcgaata gcgcagtacg aatttcccct ggaagcgctt atcgataaag tatttccgct
    24001 gccagaaata aggcttcttg ccttgttcca ttgttccgtt tgccattctg ttgaacgacc
    24061 tgagtgcgca cgcgcgtcgg tcaatcatgt tttcctgttt tcgtcaattc gaacaaaagt
    24121 cagcgcggga tgtgacataa cacatacacc tgaaacctga acgcagaaac tcccacaagg
    24181 taaatcgcaa cacggggcag ataccccggt aactaccccg gataagattg ccaagacaga
    24241 atgcttcctt acataggtta tcatgctcga gcccgccaag ctggaagcga agctgcgcga
    24301 aatacagctt ccattccagc cacaggttct ggccgcagtg ctccagttgc gcgacgacac
    24361 gcgcatggac tttatcgaac tcgacaggct tatccgctcg gaccagacca ttgcatcgct
    24421 gatactaaaa ctcgctaact cgtcgtttta ttcgcggggt aacgaaatac gcacgttgcc
    24481 acaggcgatt ggcatgattg gctttcgtac gattgtttct attgttaccg ctgcctcggc
    24541 caagaatatt ttcctctcag gtaactatgc aaagttcagg cgctacgtct ggcagcattc
    24601 acttgtaacc gccattatcg gcaagatcat ctgcgaaaaa atgcagtgga aagaggctgc
    24661 cgaagaagtt tttatcggcg gcctgctaca cgatatcggc aaagtcatac ttaaccagat
    24721 tgaccgtaag ctgtatatag acgtcatgaa tacgacgctt gactctggca aggcgttccg
    24781 gcatgcagaa aaagaaagct ttggcgtcga aagcccggtg gtcggtcagc tgatactcaa
    24841 aatctggaac ctgcctgtca tctaccgtga ggttaccgtt tttctcgata aaccttctga
    24901 ccctgccatg cagcgcctgc ccgtgcacga ccagcagatt gtgcgcatcg tagggctggc
    24961 aaaccttttg gcaaaaataa acgaatttgg acaccttgaa actgatcatg cggcactgga
    25021 atcagaatac ctgcaaccac tgggtattac gcccgacggt gcattgatga gtatcgactg
    25081 gaaaaaagcc gtcgtgaacg acacatacta ccaggtcttt tcggtaattg gctgacatga
    25141 aaactgacca ggaaaccgaa ccgcacgatc gccttttcga gattacagct tttgagctgt
    25201 attcgggcat caagaataca atccgcgatg aaatacgccg tgttgtcaca gaccttgggc
    25261 gacgccagct tgccggcccg gtgacttcga tggtcttcga actcgccgac aatgcgctca
    25321 aggcgctata ccgccacgta ttctacaagt acgcgatcgc agaaaccggc cttggcgacc
    25381 tgccctacga cgcctggtta aaggtctttc gggatgagat tgaaaccaac agcgcgcgga
    25441 attttgaacg catctgccgc caaagaagcc tcgctgtggt tgtaagactg acgcagctcg
    25501 acgaggcgct gcgcatcgaa gttatcaatg acggtgtacc cgataaatca gaacatgagt
    25561 acctgcgcaa cctgctgaaa aagtcggcga gcgcacacaa catccaggcg ttcgtcgacg
    25621 ccgagaacat tgatatagtc gatacggaaa agccaggcct tggctggacc ctgatttttc
    25681 ttacgctgcg cggcatgggg ttaacctcga gccagttcca ctttattatt ggtcacaacc
    25741 agaccaccgc gcgcatagat ttgcctctca gcgtcttcag aaccacgagc gaagcagcgg
    25801 tcaaggttgt tgaccccggc aaagtggccg atgagctttt ttcccgagtt gctaatacgc
    25861 tgaattttgg tatcgtgatc tttgaccgca agggtgacct gcttgaagtt tcgcgcgctg
    25921 tactcgacca ggtgggtctt aaacccgaac aggtggcgga gtttcccgag ctactcaagg
    25981 cacgcttcat cgaggatgtt ttttcaggcc cctttaacgt agcctcggtc taccactttg
    26041 agaactaccg cctgaaagtc gaaagcaaag atggccgaaa agaggtactc ttcaacatca
    26101 gcggcattct caaccgtgaa acccagcgcg tcgaaacgat ctggcagacg atcaacctcg
    26161 aacaggatat caaaacgctt tccgaaggtt ctgtcttcga aaatatccac atccagagca
    26221 ttgtccggcc ttacataccg aacatgattc tcgaaaaggc gcatgaaacc ctgcgccacg
    26281 gcctctttaa gctgcccaac gagcacaaag aactcacgat catgtttctt gacctgatcg
    26341 gatttacctc gaagagtgag cacctcgacc ctgcaggcgt gatcgatctg ctcaatctcg
    26401 ccatgggtat cgccgtgaag tcgattgaag tccaccatgg gtttatcgat aagtttcttg
    26461 gcgacggcat catggcgatc ttcagcgacc cgctcgaggt tgtcgtcgcc ggctttgaga
    26521 ttcagaataa tttcaagggt gtcaatgagt accgccaggt aaacgacgaa gaagcgattg
    26581 aaatgcggat tggcatcaac acaggcatgg tgattctggg aagcgtcggc accaagcgcc
    26641 gcatggactg gactgcactg ggcgatgttg tcaatactgc ctcgcgcatc gaaaaggctt
    26701 cacgcgcaaa cagcgtgctg atcagcgagg cgacgctcga gaaaatacgc gaccatgtca
    26761 cggtagctga atctttttca cagaaagtta agggtaaaca gcaggagatt atgctccact
    26821 ttctgaaatc ggtcgactac gccagcgctg gtcagaagcg caggctcgag ctctgacgac
    26881 actcattcag cgactcgaat tatctgacgc gacgagcaga aaataagcag cggttgcaac
    26941 acccagtagc atcagcaaga tgcctgcaag accataaagc aaaatacgga tatctttctg
    27001 gtagccggtg aagggattgt gcgtgaagat atacttcagt accctcgtaa tacccgcatc
    27061 ttcgagcagc gattctactt cgattgcctt acccgaaagt ttcccggaaa taatcaccag
    27121 ccagcaaccc agcacaaaga acagaatcgc gaaaaacagc ataccgcccc gtaaccccgg
    27181 tcagggcccc ggtaaagttt ttttctgcaa cgaaacagtg ttgcattgca tctaagtgac
    27241 gttgaagaaa aagaagattg taaaccgggc aaaaagcacc cggcagccaa agaaaaaagc
    27301 ggccaggcag caggctagtc gccagtcagc gcgcaccaaa ctggcagacg cgctaaaacg
    27361 gcgtgcgcac catgcgcgct tcaaacgcgc catgcggcac gagatcgcca acctcttgac
    27421 ccacggtatc ggtgcaggcc tcgcaattgc cggtacggca tttctcattg tgaagggtgt
    27481 cgggctgcat gatccgtggc gcactgtcag ctacagcctg ttcggtgctt cgatggtctt
    27541 tctctatatg gcttcgagct tttaccatgc actgtggcac ccaaaaatca aacagattct
    27601 ccgccgcatg gaccacagcg caatattttt tgccattgcc ggtacctaca ccccgattct
    27661 gctggtaacc ctgcacgggc cgctcggctg gacattcttc ggcatcgtct gggggctcgc
    27721 ggcggtcggc ataaccttta agatgctgtt tggccaccgc tatgaggccg tttctctcac
    27781 aacgtatatc ctgatgggct ggctggtgat ttttttcgtg aagcccgtct atctcggcct
    27841 ctcggtcggt ggagtctggc tacttttttc aggcggcgtg gcctacacgg caggcaccat
    27901 cttctactcg atggggcgcc tgccttacca ccacatgatc tggcaccttt tcgtgctcac
    27961 gggaagcgtg ctgcactttc tgtgtatcta cctgtacgtt tagcaggttc cggaaaaact
    28021 cccgcgccgg ttagccaaaa tattcgcgcc agcgtttatc ggcctctttg acgaacagga
    28081 tcttgcaggc ctctttgagg ccttttgagc ggcggatttg ctccttcctg gtcaccagcg
    28141 cgatctttga ccggcggcgc aggaaatctt cgagcttggt gatcatctca cgcgcggctg
    28201 cctgcttgat ttcacagcgc gtatactccg caccctcgat cagcagctct gcttcttcag
    28261 ggttttcgcg tatctcttca agcagctcga gcgcatgcga gccgtaacgc cgccagaaac
    28321 gcgtcgagag tttttccgat gatgtcggag ccgtataact gtcgagatcc atcaggcgtg
    28381 cctggtgaaa aaattcatcg cgcacagatt cgtgcggttc gccgtaccag cgccgcttgg
    28441 gaaagctcag ttgaactccc agctcttcgg caaattcgct gacttcgtcg cccacgttga
    28501 ggcagtcggt aagtttgccg ccaaagatag tgatctgctt attctgctta tctacttcaa
    28561 tttcgtgctt gcgcgaaagt tgtacccagt ctttgccgct gcctttacct ttcttcacga
    28621 caagtggccg cacaccgcag cgctctgcaa tgatgtcttc ttcggtcagc ggtttttcga
    28681 gtttcaggcg tttgttgatg ttctgcaaca caaatgcccg gtcttcgggg gttacatgcg
    28741 attcgggggt agtcacgcgt gtgtcagtcg taccgacgca cgttttagct cccatcggta
    28801 tcgcgaagaa gagacggcca tcatcggcaa aaaatgtcag cacgcgcggc tctttggaaa
    28861 gccgattgac gataaggtgt atgccctttg aaaacacatg ttcgtgctcg gtagcaattt
    28921 gcgcagtcct gttccattgg tctgcataag gaccgcaggc attcaccacg acattcgcgg
    28981 tgatgcggat tttttttcct tttacagtat ctcttgctgc aatgatccaa ttgccgtctt
    29041 tctgctgtgc gccggtcgcc tcgacgtagt tggctgcaat acagccatta ttgatcgccg
    29101 tacgcacaaa cgaaaacacg aaacgggcat cgttgtcgtg caggtatgca tctgagtatt
    29161 caaatccacc cgccgcattg gtcgtgttaa ccacaggctc aagctggttg atgcgcttct
    29221 tggtcaggaa cctgggagtt tttgtgaaga agctgccgat cagccagtag aaccatgtcc
    29281 cgagccagat gaaccagaca ggaaaacgga accccttttc aatggttgta aaaaagcgga
    29341 tttcctgtac catcgaaggg tagttgcgaa taagatgatt gcgcgacttg cagagcttgc
    29401 gcacaaggcc gaactccagg gtctcaaggt acttgatacc gccccacgcg aggttcgaag
    29461 attcctggct gacgaatgag gcaaagtcgg ctttatcgat cagcgcgacc ttcgctccct
    29521 taccggaaag cgctgcggcc gagactgcac cgttgatacc tccgccaata acaagaacat
    29581 cgaagtgccg cgtctgcagc ttctgaatat tactttcgcg aaggttcatg ccgacgaacc
    29641 cacggtgaaa tatgcgaggt caattggaat tgcggctggc gtggcacagg agccgagccc
    29701 ccgcatgaat ccggcgaacg cgctttacac gacaattatg tgtaaccggt agaccagtaa
    29761 tgaccggtgg gcggatacta tttctgggac ttctggttat gcaggtatcg tgcctgcgtt
    29821 atggcaaaat cctcggtatt tttgtactcc tgttttcgac aaactgtagc agtgacaacc
    29881 tgctggagac atcgatcaaa gagaatatca atgcccgtgg ttctgcgatt attcaaaatt
    29941 ttacgatcaa tcggatttat gtgagcacta cgggtaatgc cactgctgcc aacctgagtt
    30001 tttcactggc tgaaaaggga aagtattcgg tacacaccgg cagttgccag aattcgcccc
    30061 cgctcgtccc gatgacagct attgatgcgg gcaatatcac ggctgcgatt tccgcaactc
    30121 agctacagca tggccttaac caactaaagt tgtgctactg ggaatcagtg cgggacatgc
    30181 tttctgataa tctaggtttt gatctgacga gagatgacgc tgcacctacc gttgtctcaa
    30241 cggcacctgc gaacaatgcg tatcgtgtcg acacagacac aaatgtgcgc atcactttca
    30301 acgagcgcgt ggtcctgccg gccgacacca gtaccgcgat tcaggtaagc aacaaccagg
    30361 gtatagtgac tggaaccgtg accttcgata accaaaccaa cactctgact tttcaaccca
    30421 ctacaaattt tggcttcaca aagaacatac aagtcgatct tctcgctgga atcgccgatc
    30481 tggctggcaa tgcgatcacc cccatgacgg ttcgttttgc gacgagattt accatacagt
    30541 ggggcactct tggttcagat atggcattcg atttggccat tgacaatgcc aataacctgt
    30601 acttggcggg cacagcccaa ggtagcctct tcggacagcc ctatcaaaca tccgctcgag
    30661 atgcattcta catcaagtat gatccacaag ggcagccggt ttttgcaaga atgataggca
    30721 gtaccaacac tgcaggtacg ggcgacgaca gctttcaatc tatcttggta gacaatgccg
    30781 gcaatattct ggtgacgggt gaatctttcg gtagctcctc tttttccttt gacggtatcc
    30841 ctaagattgg cagcaatgat attattttgc taagatacag cagcgatggt acaaagctct
    30901 ccacagaact catcggcgcc acgagcaacg actctggcag ggtactgttg agaaatactg
    30961 ctaatgaaat ttacctagta ggcctcgcca ccagtagcgc aatgctaata gatgggcagc
    31021 cctttctcgg ttctaacgac atcatattgc gtaaatacga cgctactgga gtaaaaatat
    31081 tcaccagggt cattggcacg tccgccagcg aatctatctc cggagcggct ttggattcta
    31141 cgggaaatgt atacattact ggcggtacgc tcggcggcag cttctacggc acgaaccctg
    31201 acacgaattc cgacatgtac ctacttaagt atgattcaaa tggcaacatg atctggcata
    31261 gacaagtcaa taatccggga accgaacgtg acgcaaaatt gttaattgat ggcagcaata
    31321 atgtatggca atgtggttat gcaacccaag gttatgataa caactccaca ttaggaagcg
    31381 ccgatgttgt tctgacgaag tacaatgcaa atggagacaa gctattcagc aggcaatacg
    31441 gcacagaaag aaatgactat tgtggcagta ttgcgagaga ttccctgggc aatatcttta
    31501 ttagtggctc tacatcgggc aactttgggg gtccgaatgc cgatctaaca ggaacatcat
    31561 cagacatctt tcttatgcgc gtagacgcta acggtaatat ggtctgggtc aagcaatttg
    31621 gtggcgccgc tgcagatgag gcattagtaa tgaaaattaa tagtcttgat caaatctttg
    31681 tcgccggtcg aactcaatcg aacctcgaag gcaacacttt gctttcagcc tccaactttg
    31741 attttttctt aatgaagttt gatacaaacg gcaatctcta ctaatgctaa gactcttctg
    31801 gcaaaggagc aaagaactca ccagattcac agttttatta gggctggctg gaaatctttc
    31861 ggcaatgccc gacattccca aatgggaaat tactcctggc atcggtatgg gatacagcac
    31921 cattcaaggc gcgtttgacc agttttacaa accaggattc gatctgaact tttcgctttt
    31981 tgttcccaca ggacggtgga atagttttgt gcaaccaatg gcctcatttg gcagttttcc
    32041 atttcggacg gctgattcga cgaaacttac tcattacgac tttagcgtac cgctcggcat
    32101 ctactggcct gcttttgctg gcatttcgcc cttcgttgcg ataggcccgg aattctccta
    32161 ttatacgctt gcagctgcgt cgacggggct ttcgagcact gcgtaccgta ttaagcctat
    32221 ggctagcgca ggtctgttga tcccggtaaa ctatttttca tttatcaccg tgggagcaaa
    32281 atattcctat actcaactgt caggagaagc gttcggtaat atcaccctga cagctggctt
    32341 taccgttagc taccccacct atcggcggaa tgacgaggcc gtgagtaagg cccttgctga
    32401 acagagcaga gcgcaggcga tagctgccga ggttgaagca ttgttcaaag cgggccgcac
    32461 atttatcgct gaagaaaagt atgacgaggc agagcagaaa ttccgcgaga tgctggctct
    32521 taagccaggt gatgcggaag ctcgacgtga aattgaccgc gcccgggctc tcccaattct
    32581 caaggaagca aggctatacc gtgaagaggg caagggtttg tcggcgatca ggagctatga
    32641 aaaggcagca gtgtctcttg aagaagcccg aaccgaactt acagagttca ggcaagagct
    32701 gaagagctct gtacctgcgc tcaccaagtc cgcgatcgat gcttataata ccgggcaata
    32761 cataaatgct atagtcatgt tagagaggat ccaggccatc gatccggaaa acgagacagt
    32821 gaagatctat ctgccaaggg cgcgtaacag acagcgcgcc atagacaagc tcaattagac
    32881 gggctactga actaatctgc gggaacgtgg tagtccatta gcacaaacgt gacgtcgtcg
    32941 tcaaaatacg ccgggttatg ttcgcgcaga tcttgcatga tctggtctgc agttgcctca
    33001 gtatcctggt tgagcgtttt gcggataatt tcgcgcacct tttcggcgcc aaactcggct
    33061 ccggcttttg aacgagcttc aatgactccg tcggtgattt gcagcagacg caacccgtct
    33121 tgcaaagtct cttcaccctg cgtgtgtacc atgtctgaaa atactccgag aaagccgccc
    33181 ttcggcttca tttcgcgaat aagcccgctc ctacgttcaa caaagtagat cggcggatga
    33241 ccagcgctcg catggtacac ctttttggtg cgcgtattga caaagtagta gctcgcggtg
    33301 acaaagtggt tatcgacttt gtcgcagagc acctggttca atgcgcccag caagcgcgcc
    33361 ggctctgcgg caagatgcct gttgagagag aacgccatct tcaccatgct cgcaatcagc
    33421 gccgcgggaa ttccgtgacc cgaaacgtcg gcgatgagta cccctacgcc atcctcatgc
    33481 tccatgaagt cataaaaatc tcccccgacc tcggccatcg ggtggtaccg cgcggcaatt
    33541 ctgataccgg gcaaagaagg ataacggact ggcagaagcg cctgctgtac cttctgcgca
    33601 accgaaagct cacgctgcag gttctcgagc atgtctttct cgcgcacgga ttctttgagt
    33661 gaaatggccg tgcgtacgcg cgcgagcaac acctctgact cgatcggctt ggtaatgtaa
    33721 tcgttggccc cggcttcaaa gccccggatc acatcttcac ttcgattctt ggccgtgagc
    33781 atgagaatcg gtacctcgta cgaagcatac ttgttgcgga ggtaccggct gacgtcgtag
    33841 ccagacattc ccggcatcat cacatcgagc agaattgcat cgggcacagg cgaatgctcc
    33901 atgtaatcga tcgcctgttt gccgctctca gcgacgttca ccttataacc gacgagcgag
    33961 agctggttaa tcaatacctg caggttgaca ggttcatcgt ctacgagcag aatattatag
    34021 gtgcgcttcg gggtcggtgt cgtttcagct tgctgcacct gagttgccgc gcgtttctgc
    34081 atttcctgtg cgatagactc tgataaccgt tcgagcggca gaataccggt ctctggcact
    34141 tcaacgggcg gctgcacttt agcggtcgcc agctcgaacc aaaattcgct gcccacaccc
    34201 atctctgatt cgacatggat agtcgtgtgg tgcagctgtg ccagctgctg gctgaccgaa
    34261 aggccaagac caagccctcc atagcgtcgg gtgtctgatt tttccacctg ttcaaatgaa
    34321 tgaaagatgc gttctttatc gcctgccgca atgccaatac cactgtcgcg cacgctgatg
    34381 cgcattctgt catcccgcat tgccgcgctg attttgatct cgccgtgctc cgtgaacttg
    34441 atcgcattac cgacgaggtt gaagagaatc tgcgtcaggc gatcgcggtc agccataaca
    34501 gcaggaacgc ccggtgcgac ctgcacaacg aggcggagct ttttctgctc ggccgcaggc
    34561 gccatgaggg tcctgatttc agccagtaac tgctcgatat ttaccggcat tggcgtaatg
    34621 acgacctttt tgttcgcgag ctcgcttacc tgtagaatct tgtcgacaag agccgcaagg
    34681 cgaataccgc tcgaacgaat cgcctgtata ttaccgcgca gcgaatcaga gattgggcct
    34741 gcagctccat cgagcacagt accggagagg cctataatac cgttgagagg cgtcttcagt
    34801 tcgtgcgaag tcaccgcgag aaaatcatct ttcagggtat cgagttgctt tagctcctcg
    34861 ttctttttcc tgagctcgtt catcgacttg tcgattgtcg cggccatgcg gttgaaggcc
    34921 cccgcgagtt gccctagctc gtcgtgatct gtaataggta tttcactgtg tggaccttcg
    34981 ctcgcaagat tctgcactcc tagcatcagg tttgcaaccg gccgcgtgat gcggtgcgcg
    35041 atatagaaac caacggctag catcagggca ataacaagcg ctacagttgc atagagtatg
    35101 ccgcgcagcc ggataatcgg ggccagcact tcatcgagac tatagagcag ttcaacataa
    35161 agaagcggtg gtttgccggg tggcccggct tcgcctgtga agcgcaggca ccgctcgccc
    35221 ttgggcccgc aatccacctt tgcgaaagct gcagattccc atggggtaaa accggcccgg
    35281 aaaacatcgg gtaccggatt atcagcccga tcaaaacccc caacgatacg gccgtccagc
    35341 gccggatctt tggagcttaa ccaggcaagc gcacgcagca atccttcgtt ttccagcgcc
    35401 tgaaaaattg tgctcagttc acgggcatcg acgcgggctg agtttacgtc gcccgcgtcc
    35461 atcagcagaa tattcatgac gctgcgtgca tagagccgtg cctggaattc tccgcgggct
    35521 tgcgccgcca tgacacgttc attttcctgc gattggacaa tataaactga aactggcaag
    35581 atgcctgcga gaatgacggc agccagcgtt aacgaaatct tgaaccgtaa gggcacggac
    35641 ctatacgcgt atgagcaaac agactacaag cagaatcaga gctgcctgaa acgggcagag
    35701 aaaaattcca ggtccgcctg gtgctcaggc gtcatgcggt gtccgataat tcgtgaaagg
    35761 taatcttcgg caaacagtcg atcgaaaccg aaacgctgca cagcttcggc gagcagcttt
    35821 tcctggtcgt tgagccattg ctggtaagca aggtcaagca ccggctgcag cgcatgcatg
    35881 atcgcatccc ggcctaccca tagcgcgtag acgaaaccat ggtgaaagat tgtatgataa
    35941 gcggtctgca gatcataaga gggggaatgc atataccgca gcgcggtatc gcctatggag
    36001 agcagggcct cgccaggcgc aagaacagga atacgttctg ccggatcccc cacctcaacc
    36061 aattgtggag aactttgcag ctcatgcagg ataactcgca gctgcgccac agaactacgc
    36121 gaggcgacgt cagtatagat cagctcagtc tgcgcgatct tttcccgaaa agaaacatca
    36181 ttattccgcg caaagaggcg aatggatttc gactccgcct ctgccgctac gcacaaacca
    36241 ggatggtagg caaagccctc agggtgacga agatattcga cagaagaaat aagagccgca
    36301 tccagattgc ccgctcgcaa ttcgattgca agtcgcgagg gcacatcggg caccaggata
    36361 acgcgggagt catgctgcaa agccgcccaa agcggatatg cattgagaaa attgacaact
    36421 cccaggcgga atggttgcat tgaaaaatgc ggatttttta cagcacgcct gcagcagcta
    36481 agagcccggc accgatgttc aggctgagcg gcgctccatg gcagaccagc accagtttga
    36541 gtgaggtatc ggccgccagg tcactgatat atttcttcac aactggccgg tcagcaagct
    36601 gtctccactt cacaatcttt gcaataccaa aaccaccgtt cgcggtgccc atgatcagct
    36661 tcgccagttt tgcacccaaa ccagtagcgt agcagtcgtc gccggcaaac aggtcacaga
    36721 ccaccagtgc atttccaccc ggaattttga cgaacaggtg gatttcatgc atcttgagtc
    36781 caggcgccag ggtagcactg acacgtgggt cgttcaggcc ttttaccacg tcgccttcaa
    36841 cggttacgcc gttatacact tcgccaactt cagcaccgag caccttcagt ccggggtagc
    36901 gtagcttgta aaaggctaaa tccattgtat ggaagggttg cgggacaatg atcacttcgg
    36961 gtttgccaag tttttctagc tcggccattc ccgcctcatc gagggctatc gcactataga
    37021 tcagcaatcc accgctgctc agttttacaa cggtcatgtt acgcttcaat ggaaaaggca
    37081 gggaaccttc tacctgccac aatccgggag caatttccac aagtcccttg tgcggtagaa
    37141 ttttgtgcgc catgcgggtg ggtttgtctg tcactgaccg cgtcaatgac attcaaaatt
    37201 actctgagcc ggcataggcg ccacgcatcg caggtggcag cagtgcggca agtctcagcg
    37261 caatggcgtc catggcatcc tgcttgcggg tgtctttggg cagggtagcc gtgtcgatgt
    37321 gatctatact ttgcggcgct tcgaccgatg ggccgacaaa aatggggtca ccgatcgaca
    37381 tgctgccgtc ggtgatatcg aatgtaaaac tattagaatg taaaatcttg tcggcaccgg
    37441 tgatcgccac cggcaacacc acggtaccct caaggtaatt gtagagcgcg gagtgaaatt
    37501 tgcccatgcg gccatcgcgg ctgcgtgtac cttcagggta caggatcaga atgtgcccat
    37561 tgcgctgccg ttctttgccc tctttgaagg agcgtatatt cagacgggcc agctcgcgct
    37621 tgatcggttc attttccgcc atatcgcgcg gcgaagcaac cagcatcgag tggaacattc
    37681 tgccggcaac acggctgtaa tctgacgtga agacaagcct gcccgcgatg aagaatatct
    37741 tttctgcata ctgcgtgaga tgcggctcac ggtgcaagag accatagacg accgcagcat
    37801 cgaacaaact gagatggtta gagacaatgg tgacaccggt agtggtgagc atggggatga
    37861 ccttctcaaa gttcgcgaga ccgtgcacct tgtaacggct cagcagcggc gtcacgatcg
    37921 cgagcatcag gtcgtgaatt tcttcgtcgc ggcccaggta gggcgaagcg gttgcagcca
    37981 gctcggggtg gtgctcgaag tcgagcgcct cgggcatagc ggtcgttctg gcgaggcgaa
    38041 tgtagtcgtt caacagctgg cgcgcacgct cttcggtttc accacccgcg acaatcgccg
    38101 gtatactttc catgaattgg tcttcccagg aaagtttcga catggaccgc tctctcttat
    38161 agcttcagcc tgacgagaat tctgagtttc cgggcgatgc gggccatggc caactaggga
    38221 acagcaaagc agtagatacg cctgggtaca ttgcagaagc caccggacct attggtccaa
    38281 ccgaaatcga tagcagatga atccccaata gcagcttgaa tcgcaccggt ggcatcttgc
    38341 cagtttgagc aggtatttgg ctgtgcatta ccctgttgcg ccgtgccggt ccagacatca
    38401 tcaagcacga caggagctgg attacctgat tccgtaagtc gaatggccgc agaaaatgac
    38461 ggtgaggcat taaacagatt actcatgcct gagacgatcg gctgaaaact cgtatcatac
    38521 catgtgaaac tgccagctgg cgcacagcca tctcccgtcg tacccaaaat gcgacaaagc
    38581 atcgtattat tgacgggtgt gccggtttca gaaagccagg cgacaaaccg gctgccagca
    38641 ttcatgcgcc ggttatattc cctggccttc gcctggcagt agcagtcggc tcgctgtata
    38701 cccgtacccg aacaccccga agcgaacagc gcttccatgt tgccgaccgt tacactgctg
    38761 gaaacaaaca cgatcaggcg gtcggtaaat ttttctgcgc caccgggatt ttccagttgg
    38821 tccttgagat tgtagttgtt gcatgcatgc aacaagatca ggcccaatga cagcaaccac
    38881 gagggggctc gcataagaat tacctaaccc cgctcaccgc tcggtcaagc gaataatacc
    38941 accggtacgg gcataaaggc ataatgggct gcgcaaaaac gtctgatgcc attacggtac
    39001 agcgatgcag taaatacgct tgaacaggtc gcaaccgaat gttgcaccgg aagtccatcc
    39061 actactctgc gaatcagaag taccggtgcg accctgggta ccagcagcag gcgaattcca
    39121 gttattacaa gtgaatccgc cggcaatatt cccgccgatg ttagtacccg tccaaacatt
    39181 gtcagtcatc gacggtgcgg gaaagcgtcc ttcagtatat ttggccgcgc tgggcatcgt
    39241 gagcgtgctg cccaacaaac cagtcgtggc cgactcaaaa ctggtgaaaa ctggctcgta
    39301 gtttgtattg taccaaatga acggcccact cggcgtacat gtactgccac cctgattcac
    39361 catgcggcat ttcgcggcat cggcctctgt gctcaaccac gcaatgaacc gacttgttga
    39421 cgacatcctg agtccgttct gtgcagccaa agcctggcag acgcagtcgg ctttgccgat
    39481 gcccgtaccg ccgcagctgc cggcgtttag cgcgaacata tcacccgcag tcatctgcga
    39541 cgtcacaaaa acaaagaggc gatcagaaaa ggactctttg ctaccaggcc gctcgagact
    39601 gtcgagcagg tcgtagttgt tacaggccgt cactgccgcg aagagcagaa tacttaaacc
    39661 gcggacaaaa ttgcgcatgg ccgatgttca gcttccccct ttggcgggtc aacggtattt
    39721 caaggtgccg tcaattcttg atgcggatac aacatgcccg ccagggcctg tccccgtcga
    39781 gacggagaag ggcatgactt gcagagagag acatgcgccc cgagacacgt ctaacatgaa
    39841 taaaattcac ttcaccgtct aaacgagcat gggtgcatgg caatttatca tgccagtaca
    39901 aacactcctg attctgctaa gcatcggcct atcagccggc gcactcggcg gcatctttgg
    39961 tatcggcggg ggattaatca tgattccggg cctcgtgctc ttgctgggta ttgaccagc
//
LOCUS       DAOQJF010000002        26289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_820_length_26289_cov_5.124838, whole genome shotgun sequence.
ACCESSION   DAOQJF010000002 DAOQJF010000000
VERSION     DAOQJF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 26289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26289
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_26289_cov_5.124838"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLF85_00180"
     CDS             <1..724
                     /locus_tag="PLF85_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNE18095.1"
                     /translation="EDRALLSFIFARFIDGEMTGLLEGRALGYAPTLQAADFLARQVR
                     DEIRHAQMYVALYRHVNQREDVPRSPWLLKAIMAPVTGRLWAEHCFLDKAIGERWVLY
                     LMQQLIEDTQDERITRTLRAIAKDEEEHIAFGEAQTRDYANASRFRRYYLWGLFLRLD
                     LAMAIAYRLLRPLIRKRYSEAAAVMLTRFFTESRDRIAAECAALLGVSVNRSLIQMLV
                     CQLLFWIRLPFVGWFRGPRPGI"
     gene            774..1406
                     /locus_tag="PLF85_00185"
     CDS             774..1406
                     /locus_tag="PLF85_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNE18096.1"
                     /translation="MSRALDFLLKARPEPMQAYFAFLKQAGSHLDAKTAALISVITKV
                     HAKTPTGLRQYLPRALKAGASADEVIDALLMAMPALGFSKIVWAIDVILEMNLPEFAR
                     YQEAASVTSNWTELGDSSAFAAGKSTYVRSHGKTVIVRKSGEEFQVYDAHCPHKCNLI
                     PESGLAGDELVCPFHEWKFNLAGGNCTSGGKTALARLPCRVENGKLIIQV"
     gene            complement(1465..3033)
                     /locus_tag="PLF85_00190"
     CDS             complement(1465..3033)
                     /locus_tag="PLF85_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNE18097.1"
                     /translation="MSDISAEIERRRAFAIIAHPDAGKTTLTEKLLLYGGAIHLAGHV
                     RAKRDRKTTKSDFMALEQERGISVSTAALQFNYEGHVMNLLDTPGHQDFSEDTYRTLM
                     AVDSAIMLLDAARGVEPQTIKLFKICRERNLPIITFINKMDMPALEAFELLDNVEKVL
                     GISAVPLVWPMGSGKDFKGYYDLYGKKLHRFERTVGGAHRAPEAVSGIEDASVRELMG
                     DTAHDKFCEDVAIAAEALPPFDKQKFLSGEMTPVLFGSAVTNFGVEHLLANLTDLAPA
                     PRPIVLDNGETIAPGENRFTGFVFKMQANMNKLHRDRVAFVRITSGRFERGMSVNVPR
                     LGKEVKLANPVAFFGQERTTIDTAYAGDIIGIISPGLYRLGDILCNGPAPAFHPLPRF
                     APDLYARIVLTDTGKAKQFRKGISELADEGVVQLFNLSDATPLIGVIGQLQFDVFKYR
                     LEDEYGAPIRLEPLSFECSRWISKEDAQKAGRFDKVVQDEAGNPVLLFESQFRLRSFQ
                     EQNPDVTLYMHPPV"
     gene            3119..4039
                     /locus_tag="PLF85_00195"
     CDS             3119..4039
                     /locus_tag="PLF85_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18098.1"
                     /translation="MRIFFLLAFVFLPALATAAEGQILHRAAERLSQALGQAICKDLR
                     PGESQCTVSIESATHHQVGPSASLWALAWKANTVFKLKGENENTMRDALEKEQVQVLD
                     IFPDYFTGITYFRQQAEQFEQLNSAAFSMVALNTTQKAVARISPELFRNGDLQYTLPE
                     KWQTEGVSAGDRGAIILTLPRTAADGLALGLRDLDDAQLKGIRIESCSLREADKEVAC
                     NPENLRLRQKTSNITLQLAAEKKIKTSQPAFKLVLQEAGSTKFLLHLAIPFRPSPNYL
                     VFGVAGFLFGGLIAVMVLFLRRKPPNGARP"
     gene            complement(4056..4913)
                     /locus_tag="PLF85_00200"
     CDS             complement(4056..4913)
                     /locus_tag="PLF85_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18099.1"
                     /translation="MRNYLLFLLFLLPAGLLAAPAVREPVVEATIDWQNEKILLTAQW
                     QPGQALANQSNAELRQRLREALLKKLSVVVSALWERSKADGAAEPDLSALWSSLRLDT
                     FQVAENRALATMQVALRGRSSLLAHLPIEYGSELQAESGEALPAAYDKRPNVGEHDSS
                     DHEPLLYTGLVIDARHLPYVPSLNVGVFTSTGRQIYGAAFVTRATAVKRGIAGYFASD
                     SAPSALRRAGKRPLKVSAIDLQAAGKHGIVISEEDAAKLLAHSDSTRNMRRARVVIIV
                     NADKLRERY"
     gene            complement(4984..5343)
                     /locus_tag="PLF85_00205"
     CDS             complement(4984..5343)
                     /locus_tag="PLF85_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE18100.1"
                     /translation="MAAKSGIKIKVKKKGEISIVEIDGAIKSGMEFDLADELESCMHD
                     VKVPKIIVDMKKVPFINSAALGILLNIYKEIERRNGRFGLCAISSDVDHLLEITKLGS
                     VFDIYKNQDDALDSIAD"
     gene            5473..6297
                     /locus_tag="PLF85_00210"
     CDS             5473..6297
                     /locus_tag="PLF85_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18101.1"
                     /translation="MRFRAIIQARLSSSRLPRKVLEKIGSRTLLEHIAGRLESLQGAG
                     VETAFALAEDEPAELADFLGSLGYSYSIGATYDVLKRYIDASADMADHEFVLRLTGDN
                     PFPDKDQIARLVHYARSHALDYGYTADLPLGMGAELIRVNALRSVWLRTEPGVPGSES
                     AIKPHHREHVTVFIRENPHLYEIYPLRLDDSVPEEAAKDRVRGIRLTIDEAADLEVCR
                     KVYAHFERLGNPHFSALDVIQLAKNSPEMFAENLHVQQKTAQSVDERATNTARRPG"
     gene            complement(6299..8731)
                     /locus_tag="PLF85_00215"
     CDS             complement(6299..8731)
                     /locus_tag="PLF85_00215"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE18102.1"
                     /translation="MALTLDKSTLKKPMSIIFYIVSALGFASIIGIMVTPKDNLRLNV
                     MLHETYNIVLIFSTIGFIGLVYVLLDFVGFFGLDRNKFMGLYLALSIAGWFCFIYQRY
                     DFILKPLEEFGINMRFKMAAETQKYQQIGDQIVKIPLKSLHPAIHIVGIETDTVQTYG
                     GYPFSWKHYADELKSLEGSGVNTVMFDVFFMDDFKDNYGLLHTVETLRDQAPQLLASG
                     KGINISAGELKARSARWSDQIRRSANVVVDYGMETSEFDPAILQDPDLKGRIAELNKF
                     RIPDQNIVENEYLKDPYLEWVSFPFPPIPAVSRATKGLGAANVKYEASSVNYVMPMVF
                     KWQNRLYPSISLLLACRYYGVDITKDVEVKLGSYVKIKNIPDKKIAIGVFGEERDIMT
                     KPNADRSITVPINEIGMMSINFIGGPYTFPSTKMVELAEQVNRKEKTLNIEDYKNKIL
                     LTAMYYATGVSTTRDIHPSPFSHTTAGIEHHASALNTILMQDFISYASPWTNYLILFA
                     FAALLGFLIPRLNIGVVLVVVGVISGAFLVEAILVFKYFKYVHFFLVPYVQVVVILIA
                     VIGYKVLTEEENVKYIRSTFSKFVAKDIVNELLANPESLKLGGEKKEITVFFSDIRGF
                     TTMSESLSPEDLVYLLNDYLSTMTDLVISYRGTIDKYMGDAIMAFWGAPIPNEDHAYY
                     ACIASIVQLEELHKLQARWAEQGYPIIDIGIGLNSGYAVVGNMGSSHRMDYTVMGDTI
                     NLGSRLEGTNKQYGTRIIISEFTYAKVKDRVYTRELDDIRVKGKNEPVKIYELIGLVN
                     PEDAAKFRTQVT"
     gene            complement(8789..9799)
                     /locus_tag="PLF85_00220"
     CDS             complement(8789..9799)
                     /locus_tag="PLF85_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNE18103.1"
                     /translation="MQIQVENLVKTYKNFRALDNVAVDIAHGELLALLGPSGCGKTTL
                     LRIIAGLDFPEGGTIRFDGADVQTLSAKDRNVGFVFQHYALFKHMTIFDNVAFGLNVR
                     KRSERPSKKEIRDRVMELLSLVQLANLEDRYPHQLSGGQRQRVALARALAVRPRVLLL
                     DEPFGALDAQVRKELRRWLRKLHDEIHITSIFVTHDQEEALEVADRVVILNKGKVEQI
                     GSPDEVYRHPKTPFVYQFMGNVNLFHGRIEGIQGMGYVRPHEIELDRQQKFPTDFSGT
                     VEGIQPLGPYTRLTLNDQKTGQLVEAEVPYETGKALGLNIGMAVFYRPRNVQTFDGDY
                     VI"
     gene            complement(9799..10668)
                     /gene="cysW"
                     /locus_tag="PLF85_00225"
     CDS             complement(9799..10668)
                     /gene="cysW"
                     /locus_tag="PLF85_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNE18104.1"
                     /translation="MTPGQRSYLTDPLWLKAAFIAFAVVFLTLLLGLPLYEIFTQAFQ
                     KGLDFYLEALSDPVTLASIKLTLVAAAISVPLNLVFGVVAAYAIAKFDFRGKSLLVSL
                     IDLPFAISPVISGLIYVLVFGLQGWLGGTLQEAGIRIIFAVPGIVLATVFVTFPFVAR
                     ELIPLMEEQGRSEEEAALMLGASPLKMFFMVTLPNIKWGLIYGVILCNARAMGEFGAV
                     SVVSGHIRGETNTMPLHVEILYNEYNFTAAFAVASLLAFLALATLLVKTAVELRARLA
                     QRGHLQESAGGLS"
     gene            complement(10665..11510)
                     /gene="cysT"
                     /locus_tag="PLF85_00230"
     CDS             complement(10665..11510)
                     /gene="cysT"
                     /locus_tag="PLF85_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNE18105.1"
                     /translation="MASESVLRFSFREKSILPGFGATLTYTVIYLLVIVLIPLGAMFA
                     KSFSLGWHEFAEHALAERTLLSLRLSFTTAFVAALINMVAGFIIAWVLTRYDFWGRRL
                     LDTMVDLPFALPTAVAGIALCTVYAENGILGSLLGRAGIKAAYNPTGITIALVFIGLP
                     FVVRSVQPVLEDFEKEIEEAAMSLGASRLQTIARVILPSVAPALFSGTIMAFSRALGE
                     YGSVIFIAGNIPFKSEIAPLIIITKLEQHNVQGATAVAAMMLLISFSLLLLLNYVQIR
                     QKRKV"
     gene            complement(11500..12465)
                     /locus_tag="PLF85_00235"
     CDS             complement(11500..12465)
                     /locus_tag="PLF85_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE18106.1"
                     /translation="MVAAAACGPKKQGRELLNVSYDPTREFYREFNEAFAREWYKSHP
                     GETISFRQSHGGSGKQARSVIDGLEADVVTLALSSDIDTIAKQAKLLPSDWQKKLPQS
                     SAPYTSTIVFVVRSGNPKGIKDWSDLAAPGVGVITPNPKTSGGARWNYLAAWAYAEKK
                     FAGNKNQISAFVKSLYKNVVVLDTGARGATTSFTQRKLGDVLIAWENEAYLALKEFPN
                     QFEVVVPSLSILAEPSVAVVESVARKKGNLELASAYLQYLYSDEGQDLAGKHYFRPRR
                     QEIVKKYAHIRPLALVTIEDAFGGWDKANKTHFADGAIFDQIYGK"
     gene            complement(12565..13551)
                     /locus_tag="PLF85_00240"
     CDS             complement(12565..13551)
                     /locus_tag="PLF85_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE18107.1"
                     /translation="MKKSAFKTIVLLFASATAIIAKDYELFNVSYDPTRELSREFNAA
                     FLRHWKQKNPSDNIRFKQSHGGSGKQARSVIDGLDADVVTLALAGDVDVIAEKAKLLP
                     TDWQKRRPNNSAPYTSTIVFVVRKGNPKGIKDWDDLVKPGTAVITPNPKTSGGARWNY
                     LAAWAFAQEKKLDPRTFIKALYRNVAVLDTGARGSTTTFTQRKIGDVYISWENEAFLV
                     SKEFAADGYEIIVPTLSILAEPSVSVVDANANRKGNAEIAKAYLDYLESPEGQNIAAK
                     HYYRPRNPKAAARFSHFKKLKLITVDGAFGGWKNAQKTHFDDGGTFDQIYGK"
     gene            complement(13586..15178)
                     /locus_tag="PLF85_00245"
     CDS             complement(13586..15178)
                     /locus_tag="PLF85_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNE18108.1"
                     /translation="MKIKRFLVYLAALVVSLQGAALRAQAKKPKAAVQQAETLSDEEA
                     IPPEVSEADKTAATVEVRDSYYVRSPSYGTEPIDSTQPAYAKRLSETGIGVFRNIDWI
                     IFGVEQRTRGEFRENDFRRPNSPVNPQGPSGNPATQETPLLFKTRAFLKLEFDWFRLV
                     TEVQDSRRTPIKTGADTRDFNEFEPIQAYAEVHFRNLFGYNRPLAVRGGRYALELIDR
                     RLVGLNEWRNTTNTFQGGWISIGRKENNWDLNLIAAQPLVREIEKLDQPIGQQWFYGG
                     VLSVREWSQWVTFQPYYFGLNQDNQPARSDWYPTSDAGARFFRNIHTVGGRAYAALGK
                     TGFDYDLSYTKQFGVDQLGNNAVERKQDAFNTTAEVGYTFKHPWKPRLAGFYAYISGD
                     IDRNDNTQNRFERLYGFARPWSANDYFQLENLETPKVILEFEPIEKLKIDTAYVWYWA
                     ASPTDRWNNAGILNTATPAGNANTDGKFGEEYNIRIRYPFPHLKVNIGYAYFRAGSYT
                     EKFKRPGDSHFAYLELSALLFD"
     gene            complement(15398..16312)
                     /gene="cysK"
                     /locus_tag="PLF85_00250"
     CDS             complement(15398..16312)
                     /gene="cysK"
                     /locus_tag="PLF85_00250"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNE18109.1"
                     /translation="MKVHSILELIGNTPHVKLGRLFAAEHEVWVKLERQNPGGSIKDR
                     IALSMVEDAERRGILKPGSVIIEPTSGNTGIGLSLVAAAKGYKIILVMPESMSVERRA
                     IMASYGAQFDLTPREKGMKGAIERANELAAQTPGSWVPQQFENEANITAHAKFTAQEI
                     LRDFPEGIDVLITGVGTGGHITGVGEVLRQKWPNLQIFAVEPAASPVISGGSPAPHPI
                     QGIGAGFIPKNLRKEILTGVIQVTKEEAFEYAKRAAREEALFAGISSGASLAAVAKKL
                     PELKKGARVLTFNYDTGERYLSIEGLFG"
     gene            16533..17165
                     /locus_tag="PLF85_00255"
     CDS             16533..17165
                     /locus_tag="PLF85_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNE18110.1"
                     /translation="MPARKSRAASEKPAGGKSRAQTGENLTGVVADNVKRLRSDLDLS
                     LDKLANLSGVSKAMLSQIEQSRSAPSINVLWKIARALDVPFAALISKRTDAQLQVVRR
                     NEMKILSSQKGGFVSRALFPLDIPRRVEFYELEMSQGCVEKAVAHPPGTAENIVVAEG
                     QIEIEINGQKNLLEQGDSIYFVADQPHVYRNLAKGTSRAYLVMTYAEKRV"
     gene            17230..18717
                     /locus_tag="PLF85_00260"
     CDS             17230..18717
                     /locus_tag="PLF85_00260"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNE18111.1"
                     /translation="MQIFNFQGYSLEAILLAAALLVTLSVIASRISSKFGIPLLLVFL
                     GIGMLAGSDGPGGIAFENYRMAFAVGSACLALIVFDGGMSTSWNSVRPILRQGISLSV
                     LGTFLTGAATAAFAHFVLHMHWSDSVLLGAIVSSTDAAAVFSLLRARSLGLKGTVKQT
                     LEFEAASNDPMAIFLTVGVLLFLSNPGASIVDFLKLLVMQGGIGLALGLLGGRAIRWL
                     INNAGIEYEGLYGVLALGLIVGLFSLTGLIGGSGFLAVYVAGIILGNYEMLHKASIAR
                     FLDGVAWIAQILVFLTLGLLSFPTRVFPVWQEGLLLAVFMMFVARPLAVFLAVPGRGM
                     TLRERTFVSWVGLRGAAPIILATYPWSIHFPRAEYLFDLVFFVVISSVIAQGISIPWL
                     AKKLGITVPLAKPRRIDLAHGVLPQGFLSIEIHVTDDAAATGQKVVDLELPSGVILTS
                     IERSDRFLIPRGDTLIEAGDRIFGLARQTNLPALQDIFGKTEVVA"
     gene            complement(18734..19663)
                     /locus_tag="PLF85_00265"
     CDS             complement(18734..19663)
                     /locus_tag="PLF85_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE18112.1"
                     /translation="MQKFTRILYATACAGDDRPAMRQAVSLARNNGAHLQALVVRPQL
                     PESLSQHGSGYDAYLKSRTAAALAEVAHELTCGDLQVTVEIVTSDEPAKLIIQHMLRG
                     HFELLIKEADSTESGQGFEPVDMALLKYCPGAVWLSRPIAHHRENIRVAVAVDAEASA
                     EPALQLTLRLLQLSRSLADQCDGELRIVSCWEYELEGFLRRNSWISLPAEDVSSSVLR
                     AGDLHLAKLGELVQKAGISGRQNVHHPRGRAGELIPHFCTEHKIDILVMGTVARTGIP
                     GFLFGNTAENIVRKLNCSLLALKPEGFVSPVEI"
     gene            complement(19679..20113)
                     /locus_tag="PLF85_00270"
     CDS             complement(19679..20113)
                     /locus_tag="PLF85_00270"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE18113.1"
                     /translation="MATSNNGPLGLRLVFLLLGTLFGYILIRAGVSSYDVIREMFLLQ
                     SFHMYGVLGVAVPVSFICVQLWKKWGRKPLLAEETDWSVEKLSRKHVIGGLISGAGWA
                     LTGACPGPALAILGYGLMSGVFIVTGIFAGTWFFAWLEERKG"
     gene            complement(20113..20610)
                     /locus_tag="PLF85_00275"
     CDS             complement(20113..20610)
                     /locus_tag="PLF85_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE18114.1"
                     /translation="MTAGYIWPFWLGGLAIAGVSVALVILTGRFLSVTRGYASVCSIF
                     SRMKFFQRPDLGGPFGFRTFFTLGLLAGGAIAAFTTTGWQPTFALGKFDQIWGDNLAV
                     KAGVLIAGGFLWGYGSRMAKGCTSGNSISGLSKGSVASLVATCGFLVAGVAVTFLLAA
                     VSGVK"
     gene            20778..21821
                     /locus_tag="PLF85_00280"
     CDS             20778..21821
                     /locus_tag="PLF85_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE18115.1"
                     /translation="MAENSQVSLIGGDPIQPGLKLKGVLRSLALAVIKDERDIDLLTC
                     MIKLSILLPGSAAAILLLPEYRLYLGIAHIVLFLLYLGPYTLMLHNVSHRPLFHYHLR
                     WLNVWPQALLAFFFGMTPYTYFSHHLGMHHPENNMPEDLSSTMKYQRDSFLGFLHYYL
                     SFAVLGIFTLSLYFIRHGRWRFFRMAVLGELSWYAAVALAFWYLPEPTFIVLILPWLL
                     TRFLLMAGNWTQHAFVDTTDGNNPYVNSITFIDSPYNKRCFNDGYHIGHHVNMNRHFL
                     DMPQDFLDNLSTYRENKAVIFRKLDYFMIWFLLMTKQYRILSRFFVNISAKEMSRDEI
                     IAFLKSRLKPLRA"
     gene            21837..22880
                     /locus_tag="PLF85_00285"
     CDS             21837..22880
                     /locus_tag="PLF85_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18116.1"
                     /translation="MPILLPIIAQLGPEVEAIRLPGFDSSTLPYLVYFLLAVIGGLLF
                     LQWRLRRRAEKNALSNRFMQIMLHRNLTKRQYRAVNLFFQSLKEAEQNEILLSQKALA
                     HHLHQFLRVHEQIPANDRVEIFDKILPGMTSQIEIKTVDDLRTGEPCAVDIDHTSHLA
                     TILKTKDNQVLVSLTEKIVLPLGPAKLYAYRPNLGGFLLSGAISKVNGMSAIFKQEGP
                     IEFRGDQHLMSIVNVALKLEPWPKPEVEADLEPGEPPVESPYLLAETERLSDRALAIR
                     FLETPPALILKRQEFWEMTLELPSEPLVCRVRVTPYKPPEYWLVRPVDMDDLGRRRLY
                     RFISENKPVREHF"
     gene            complement(22893..24272)
                     /locus_tag="PLF85_00290"
     CDS             complement(22893..24272)
                     /locus_tag="PLF85_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE18117.1"
                     /translation="MEIEIINPANGKSLGKVPADDHASIARKYEAARTAQPAWQNLGL
                     AKRLEIIRRFRDRVEKDIEEAALLLTHDVGKPIAQSRNELRGLLARFDFFLAETEKTV
                     GAQTVLRDGHALVEEIEHEPLGVIGNISAWNYPWFVGSNVYVPALLTGNAVLYKPSEF
                     ALQSGLLMARYLHEAGVPEDIFAVVVGRGDAGAILLEQPIDGIFFTGSVATGRKIAEA
                     AAKKFIKVQLELGGKDPAYITDDVDVKAAAEATADGAMYNTGQSCCSVERIYVHQKIY
                     EEFMRHFEQAVRSFRLGDPEQAETYIGALTRGKPAYDFLKSQIDDAVTKGGKLVCGGK
                     LVAQGGGFFLEPTVIANADHSMALMREESFGPVIGIMKVKDDAEAQELMNDTEYGLTA
                     AVYCRDENRARGILRGVNAGSAYINCCDRVSPRLPWSGRRNSGVGLTLSTYGIEAFTR
                     PKAWHIRAG"
     gene            complement(24279..25061)
                     /locus_tag="PLF85_00295"
     CDS             complement(24279..25061)
                     /locus_tag="PLF85_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804853.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNE18118.1"
                     /translation="MERLKIGISACFMHKDPTRAIFKGKTLLYVEESLAHWVMRLGAQ
                     AFMIPSVAPGGKMQLAEYAESLDGLVLHGGADVAPESYGEKPLKDEWKGDRVRDLYEI
                     ELFKAFKAAEKPVLGICRGAQLINVALGGSLYQDINTQIDKSLVHRDWNVYDQNFHEI
                     EFKHGSYLEKVFGRGSFRTNSVHHQALKDIGEGLAVEAISTEDKIVEGIKYQGAPYIC
                     GVQWHPEFHDDEDNALLSGDPLLKDFMQECEVRRTELRMQRR"
     gene            complement(25146..25346)
                     /locus_tag="PLF85_00300"
     CDS             complement(25146..25346)
                     /locus_tag="PLF85_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18119.1"
                     /translation="MNYQVVEEIECSSCGEKVSPSVARVAGDADFDDCTSDLAFLCPV
                     CFIQAKNAAMQASLVGAALSRG"
     gene            complement(25509..>26289)
                     /locus_tag="PLF85_00305"
     CDS             complement(25509..>26289)
                     /locus_tag="PLF85_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE18120.1"
                     /translation="YEDEKPGARPFDQEIPYWIAIPTTSGTGSEVGRSTVVADDETHV
                     KKVIFSPRLLARAVFADPEMTSAMPPHVTAATGLDAMTHLIEAFLAKGWHPMADGIAL
                     EGLRLAAGALPRAFKNGGDLGARGEMLMASMMGAVAFQKGLGVVHSCAHALSAIVDMH
                     HGLANGIMLDHALAFNAKAVPDRFDRLAQAVGLAKGSEFLGWLAHLKAELKIPATLKD
                     AGVAADKLDALVRVAVADGCHPSNPVAVSEKDFSDIFRQVL"
BASE COUNT         5872 a         7421 c         6962 g         6034 t
ORIGIN      
        1 ggaagaccgg gcgctgctct cattcatctt cgcccgtttc atcgatggcg agatgaccgg
       61 cctgctcgag ggtcgcgcgc tcggctatgc accgacgctc caggcggcag atttcctggc
      121 gcgccaggtt cgcgatgaaa tccgccatgc acagatgtat gttgcactct accgtcatgt
      181 caaccaacgc gaagatgtgc cacggtcgcc ctggctcctg aaggccatca tggctccggt
      241 taccgggcgg ctatgggcag agcattgttt tctggataag gcaatcggcg aacgctgggt
      301 gctctacctg atgcagcagc tgatcgaaga cacgcaggat gaacgcatta cacgcacgtt
      361 acgcgcgata gcaaaagacg aagaggagca tattgctttt ggcgaagcgc agacccgcga
      421 ttatgccaac gcgagcaggt ttcgccgcta ttacctctgg gggctcttcc tgcgccttga
      481 cctggcaatg gcgatcgcgt accggttact gcgcccgctg atccgcaagc ggtactcaga
      541 ggcggcagcc gttatgctca cgcggttctt cacagagtca cgcgacagga ttgccgccga
      601 atgcgcagcc ctgctcggag tcagtgtcaa ccggtcgctg atccagatgc tggtttgcca
      661 gttgttattc tggatacgcc tgccgttcgt cggctggttt cgcggcccca ggcccggcat
      721 ataatactaa cgtgaaattc ttgccgggct gatttcgctt caaagcatca cgcatgagcc
      781 gtgcactgga tttcttactt aaggcccgac ctgaacccat gcaggcctac ttcgcctttc
      841 tcaaacaggc gggcagccat ctcgatgcca agactgcagc gctgatttcg gtaattacca
      901 aggtacacgc caaaactccc actggtttac gccagtacct gccgcgtgcg ctcaaagcgg
      961 gtgccagtgc agatgaggta atcgacgcgt tgctcatggc gatgcctgcg ctgggcttca
     1021 gcaagattgt ctgggcaatc gacgtcattc ttgaaatgaa cctgccggaa tttgcccgct
     1081 accaggaagc tgcttctgtt accagtaact ggactgaact cggtgacagt tctgcattcg
     1141 ccgccggtaa atcaacctat gtgcgctcac atggcaagac cgtgattgtg cgcaagagcg
     1201 gtgaggagtt ccaggtctat gacgcccact gcccgcacaa gtgcaacctg attcccgaaa
     1261 gcgggctggc cggtgatgag ctcgtgtgcc cctttcacga atggaaattt aaccttgccg
     1321 gcggtaactg tacctctggc ggcaagactg cgctggcacg cctgccctgc agggtcgaaa
     1381 atggtaagct gataatccag gtatgaactc gcaggctcct gcaaaaggag ccaagagccg
     1441 gcgcggtagt ttttccggaa cctgctagac cggcggatgc atatacaggg tcacatcagg
     1501 attctgttcc tggaatgacc tgaggcgaaa ctggctctca aacaacagca cggggttgcc
     1561 tgcctcgtcc tgcaccacct tatcaaacct gcctgccttt tgcgcatcct ctttcgagat
     1621 ccagcgcgaa cattcgaagg ataagggttc gagccgtatc ggcgccccgt actcgtcttc
     1681 gagacggtac ttgaaaacgt cgaactgcag ctggccgatg acgccaatca acggtgtcgc
     1741 atccgagagg ttaaagagct gcacgacacc ttcgtctgcg agctccgata tacctttccg
     1801 aaattgcttg gcttttcccg tgtcggtcag cacgatacgg gcataaagat cgggcgcaaa
     1861 acgcggcagt ggatggaatg cgggtgccgg gccattgcat aagatatcgc cgaggcgata
     1921 gagtcccggg ctgatgatac cgatgatgtc accggcatat gcagtgtcga tagttgtccg
     1981 ctcctggccg aagaaggcaa ccgggttcgc cagtttcact tctttaccta gccgtggcac
     2041 attcactgac atgccgcgct cgaacctgcc gctggtaatg cgcacaaacg ccacgcggtc
     2101 acggtgcagc ttgttcatgt tggcctgcat cttgaaaaca aacccggtga agcggttttc
     2161 gcccggcgcg atagtttcgc cgttgtcgag aacaatggga cgcggcgcag gtgcaaggtc
     2221 tgtcaggttc gcgagcaggt gctcgacacc aaagttcgtg accgcgctgc cgaacaaaac
     2281 cggggtcatc tcgccgctca aaaatttttg tttatcgaac ggaggcagtg cctcggcggc
     2341 gattgccacg tcttcgcaga atttgtcgtg cgctgtgtcg cccatcagct cacgcactga
     2401 agcatcttcg ataccagaaa ctgcctctgg ggccctgtgc gcgccgccga cggtgcgctc
     2461 aaaacggtgc aatttcttgc cgtacaggtc gtagtaaccc ttgaagtctt tgcccgaccc
     2521 catcggccag acaagcggca cagccgagat cccgagcacc ttttcgacat tgtcgagcag
     2581 ctcgaaggcc tcgagcgcag gcatatccat cttgttaata aaagtgatga tgggcaggtt
     2641 gcgctcgcgg cagatcttaa aaagtttgat ggtctgaggc tcgacaccgc gcgcagcatc
     2701 gagcagcata atggcagaat ccaccgccat gagcgtccgg tatgtatctt cactgaaatc
     2761 ctggtgtccc ggggtatcga gcaggttcat gacatggcct tcgtagttga actgcagtgc
     2821 cgcggtactg actgagatgc cacgctcctg ttcgagcgcc atgaagtctg atttggtcgt
     2881 cttacgatcg cgttttgccc tgacatgccc cgcaaggtga atcgccccac cgtaaagcag
     2941 cagcttctcg gtcagggtcg ttttacccgc atccgggtgc gcaataattg caaatgcacg
     3001 ccggcgttca atttcagctg agatatcgct cacctgcgga gatttgtgaa gcggaaacag
     3061 cgcgtaaatg atttgcaaat tggcttaacg gcgcgtctgc cctctggcta cagtcgctat
     3121 gcgtattttt ttcctgctcg cattcgtctt tctgcctgcg ctcgcaacag cagccgaagg
     3181 gcaaatactg caccgggcgg ctgaacgcct ttcgcaagcg ctcgggcagg ccatctgcaa
     3241 agatctgcgc cctggcgaat cccagtgcac cgtcagcatc gaaagcgcca cgcaccacca
     3301 ggtaggtccc agcgcatcgc tctgggcgct tgcctggaag gcaaacacgg tctttaagct
     3361 gaagggcgaa aacgagaaca ccatgcgcga tgcgctcgaa aaagagcagg tccaggtgct
     3421 ggacatcttt ccggattatt ttacgggcat aacctatttt cgccagcagg cagagcaatt
     3481 cgaacagctg aacagtgcag cgttttcaat ggtggccctg aatacaacac agaaggcagt
     3541 ggcacgtatt tcacctgaac ttttccgcaa tggcgatctg cagtatacct tacccgaaaa
     3601 gtggcagaca gaaggcgtta gcgcgggcga ccggggagca atcatactga cgcttccacg
     3661 cactgctgcg gatggactgg cgctggggtt acgcgatctt gacgacgctc agctcaaggg
     3721 tattcgtatt gagtcgtgta gcctgcgcga ggctgacaaa gaggtcgcct gcaatccaga
     3781 gaatctcagg ctgcgccaaa aaaccagcaa cattaccctg cagctcgccg ccgagaaaaa
     3841 gattaagact tcgcagccag cgttcaagct tgttctgcag gaagccggca gcacgaagtt
     3901 cttgctccac ctggcaattc cctttcggcc gagccccaat tacctggttt ttggtgttgc
     3961 aggattcctg tttggcggcc tgatcgccgt aatggttctt ttcctgcgaa gaaagcctcc
     4021 taatggtgca agaccctagc aggttccgga acctgctagt aacgctcacg cagcttgtct
     4081 gcgttgacaa taattacgac gcgcgcacgc cgcatattac gcgtgctgtc tgaatgcgcc
     4141 agcagttttg ctgcgtcttc ttcgctgatg acaatgccat gtttgcctgc agcctgcagg
     4201 tcgatggctg atactttcag cggccgtttt cccgcacggc gcaatgcaga aggcgccgaa
     4261 tcagaagcga aataaccggc gatgccgcgt ttgactgcag ttgcacgcgt cacaaaagcc
     4321 gcgccataaa tctgccggcc ggtcgaagta aatacaccga cgtttagcga aggcacatag
     4381 ggtaaatgcc gcgcatcgat cacgagtccc gtatatagca gcggttcatg atctgaacta
     4441 tcatgctccc ccacattggg cctcttgtca taagccgctg gcaatgcttc accgctttca
     4501 gcctgtaatt cgctgccgta ctcgatggga agatgcgcca gcaatgagct ccgcccgcgc
     4561 agtgctacct gcattgtagc cagtgcacgg ttctcggcca cctggaacgt atccagtctc
     4621 agggatgacc aaagggccga aagatcgggt tctgcggcgc catctgcttt tgaacgttcc
     4681 cagagcgcgc taacaactac gctcagtttt tttaagaggg cttcgcgcag gcgctggcgc
     4741 aattcagcat tcgattgatt cgccagagcc tggcctggct gccactgcgc agtcaggagg
     4801 atcttctcgt tctgccagtc gatagtcgct tcaactaccg gctctctgac tgcaggagcg
     4861 gctaagagcc ctgcgggtag cagaaataac agaaagagga gataattccg cactatttat
     4921 tttcgtgcta tttcggcaaa aactcgtcta actttttgca ggcagccctg caggcatgcc
     4981 ggatcagtcg gcgatcgaat cgagcgcgtc gtcctggttc ttgtaaatat caaaaacaga
     5041 acccagtttg gtaatttcga gcaggtgatc cacgtctgaa ctgatggcgc agaggccaaa
     5101 acggccgttg cggcgctcaa tctccttgta gatattgagc agtatgccaa gcgctgccga
     5161 attgatgaac ggcactttct tcatgtcgac aataatcttg ggtaccttaa cgtcgtgcat
     5221 gcacgactcg agttcatcgg ccaggtcaaa ttccattcct gactttatag ccccgtcaat
     5281 ttcgacgatg ctaatctcac cttttttctt gactttaatc ttaatgcctg acttggcggc
     5341 cattggtcta tatcaccggc tttttgttgc agacaagtac tttttgattt tttgctgaaa
     5401 aataggcaaa atgagcatgc gacataattc atcttgccat cggttcggct gtcaggaatt
     5461 aatccgctgc acatgcgctt tagggcaatc atacaggcaa ggctgtcgag ctcccgcctg
     5521 ccgcgcaaag tactcgaaaa gatcggttcc cgaaccctgc tcgagcatat tgccggaagg
     5581 ctggaaagct tgcaaggcgc cggcgtagaa actgcttttg cgctggccga agatgaacct
     5641 gcagaactcg ccgattttct cggcagcctg gggtacagct actctatcgg cgccacctat
     5701 gatgtgctga agcgctatat cgatgcctct gccgacatgg cggaccatga atttgtgctt
     5761 cgcctgacag gcgacaaccc gtttcctgat aaggaccaga tcgcgcgact ggtgcattat
     5821 gcccgcagcc atgcgcttga ttacggctat actgcggacc tgcctctcgg tatgggagct
     5881 gaacttatac gcgtcaatgc cctacgctca gtctggctgc gcactgagcc cggggtacca
     5941 gggtcagaaa gcgcgatcaa accccatcac cgcgaacacg tgacggtctt catcagggaa
     6001 aatccgcatc tctacgaaat ttatcccttg cggctcgatg attctgtccc cgaagaagcg
     6061 gccaaagacc gcgtgcgggg tatcaggcta accattgatg aggccgcaga tcttgaggtt
     6121 tgccgcaagg tttatgcgca tttcgaacgg ttgggaaatc cgcatttcag cgcgctcgac
     6181 gtcatacagc tcgcaaagaa cagccccgaa atgtttgccg aaaatctaca cgttcagcag
     6241 aagacggcgc aatccgttga cgagcgcgcc acaaatacgg cgcgtcgtcc cggttagatc
     6301 aggtcacctg agttctgaac ttagccgcgt cttcggggtt gaccaaaccg atgagctcgt
     6361 aaattttaac cggctcgttc ttgcccttaa cgcgtatgtc atcgagttcg cgcgtgtaga
     6421 cacgatcttt gaccttggcg tacgtaaact cggagatgat aattcgcgtt ccgtactgct
     6481 tgttggttcc ttcaaggcgg ctaccgaggt tgatcgtgtc tcccatcacg gtataatcca
     6541 ttcggtgcga tgaacccatg ttacccacaa cggcatagcc ggaattgagg ccgataccaa
     6601 tgtcgataat cgggtaacct tgttctgccc agcgtgcctg cagtttatgc agctcttcca
     6661 gctgcacaat cgaggcaatg cacgcatagt atgcatgatc ctcgttggga atcggagctc
     6721 cccagaacgc catgattgca tcacccatgt acttatcgat cgtgccgcgg tagctgatca
     6781 caaggtcggt catggtcgac aggtaatcgt tgagcaggta aacaaggtct tccggcgaaa
     6841 gggattcgga catcgttgtg aagccgcgga tatcggaaaa gaatacggta atttctttct
     6901 tttcaccccc gagcttcagc gactcggggt tcgccagaag ttcgttcaca atatctttcg
     6961 cgacaaactt tgagaacgtg ctgcgaatgt attttacgtt ctcttcttcg gtaagaacct
     7021 tgtaaccgat aaccgcaatc aggatcacaa cgacctgcac gtacggcacc aggaagaagt
     7081 gcacgtactt gaagtactta aagaccagga ttgcctcgac caggaatgct cctgaaatga
     7141 caccgacgac aacgagcacg acgccaatat tcaggcgtgg aatcaggaaa ccgagcagtg
     7201 cggcaaaggc gaacaggatt aggtagtttg tccatgggct ggcataactg ataaagtcct
     7261 gcatgagaat cgtgttaagc gcgctggcgt ggtgctcgat tcccgcggta gtgtggctga
     7321 acggtgacgg gtgaatatct cgggtggtcg atacacccgt tgcatagtac atcgcagtga
     7381 gcagaatctt gttcttgtag tcttcgatat taagagtctt ttccttgcgg ttgacctgtt
     7441 ctgccagttc gaccatcttc gtagatggga aagtatacgg accgccaatg aagttaatcg
     7501 acatcatgcc gatctcgttg atcggcacgg taatgctacg atcagcattc ggcttcgtca
     7561 taatatcgcg ctcttcacca aagacgccga ttgcaatttt cttatccggg atgtttttaa
     7621 tcttcacgta agaaccaagt tttacctcga catctttcgt gatatcaacg ccatagtagc
     7681 ggcaggccag caacagtgag atcgagggat acaggcggtt ctgccatttg aacaccatgg
     7741 gcattacata attcactgat gaagcctcgt acttcacgtt cgcggcaccg agacccttcg
     7801 ttgccctgct gactgcggga atcgggggaa atgggaacga aacccactcg aggtacgggt
     7861 ctttcaggta ttcgttttct acgatgttct ggtcgggaat gcggaactta ttcagctctg
     7921 caatgcgccc cttaaggtcc ggatcctgca gaatggccgg atcaaactcg ctggtctcca
     7981 taccatagtc gacaaccacg ttggcagagc ggcgtatctg gtcgctccag cgcgccgaac
     8041 gggctttcag ttcgccggca gaaatattga tgcccttgcc agaagcaagc aactgaggtg
     8101 cctgatcgcg cagggtttca acggtatgca acaggccata attgtccttg aaatcgtcca
     8161 tgaagaaaac gtcaaacatc accgtgttga cgcccgaacc ctcgagagac ttcaactcat
     8221 cggcatagtg tttccagctg aacgggtaac cgccgtacgt ttgtaccgta tcggtttcaa
     8281 tgccgacaat atggatcgcc gggtgcagac tcttgagcgg gatttttaca atctggtcgc
     8341 caatctgctg gtacttctgt gtctccgccg ccatcttgaa gcgcatattg atgccgaatt
     8401 cttcgagcgg tttcaggatg aagtcataac gctggtaaat gaagcagaac caaccggcaa
     8461 tcgatagtgc cagatagagg cccatgaact tgttgcgatc gagaccaaag aaaccgacaa
     8521 aatcgagcag gacatatacg agaccaataa aaccgatcgt cgagaagatg agcacgatat
     8581 tgtaggtttc gtgcagcatc acgttgagcc gcaggttatc tttcggggta accatgatgc
     8641 caatgatgct cgcgaaaccg agagcgctca cgatgtagaa gatgatcgac atcggttttt
     8701 tcaacgtcga tttatccagg gtcaaagcca tgggcggcga taccaaaccc ggcagccctg
     8761 acaaccaaaa actttggcac taagcctgtt agatcacgta gtcgccatca aaggtctgga
     8821 cattgcgcgg ccggtagaag actgccatgc cgatattgag ccccagcgct ttgccggttt
     8881 cgtaaggcac ctcggcttca accagctgtc ccgttttctg gtcgttaaga gtaagccgcg
     8941 tgtaagggcc gagaggctga atgccttcaa cagttccgga aaagtcagta gggaattttt
     9001 gctgtctgtc gagctcgatc tcgtgcgggc gcacataacc cattccctgg atgccttcaa
     9061 tgcgcccgtg gaacagattc acgttgccca tgaactggta gacgaaaggt gtcttcgggt
     9121 ggcggtatac ttcatcaggg ctgccgatct gctcgacctt acccttgttc aaaatgacga
     9181 cacggtcggc aacttcaagc gcctcttcct ggtcgtgcgt gacaaagatg cttgtgatgt
     9241 gaatctcgtc gtgtagcttg cgcagccagc ggcggagttc tttgcgaacc tgtgcatcga
     9301 gcgcaccgaa aggttcatcg agcagcagta cgcgtggcct taccgccagc gcgcgcgcca
     9361 gagccacgcg ctgccgctgc cctcccgata actggtgggg ataacggtct tcgagattgg
     9421 ctagttgcac aagcgaaagg agctccatca cgcggtcgcg gatctctttt tttgacggcc
     9481 tttcgctgcg tttgcgtaca ttgagcccaa aggcgacgtt gtcaaaaatc gtcatgtgct
     9541 taaacagcgc atagtgctgg aagacgaagc ctacattacg gtctttggcg ctcaaggtct
     9601 gcacatcagc gccgtcgaaa cgaatggtac caccttcggg aaaatccagc ccggcaataa
     9661 tacgcaacag ggtcgttttc ccgcagcccg aaggccccag cagagccaga agctccccat
     9721 gagcaatatc tacggcaacg ttatccagtg cccggaaatt cttgtaggtc ttgaccaggt
     9781 tttctacctg aatttgcatt agcttaatcc ccccgcgctc tcctgcaggt gtccccgctg
     9841 cgccaggcgc gccctgagct cgaccgcggt ttttaccagc agggtcgcga gcgccaggaa
     9901 cgccagcagc gacgccacgg cgaaagccgc agtgaagttg tattcattat agagtatctc
     9961 gacatgcagc ggcatggtat tggtctcacc gcggatgtga ccagaaacca ccgataccgc
    10021 gccaaattca cccatagcac gggcattgca gagtatgacg ccgtagatga gtccccattt
    10081 gatattcggc agagtgacca tgaagaacat tttgagcggc gacgcgccaa gcatcagtgc
    10141 ggcctcttct tcacttctgc cctgctcttc catcaggggt atcagctcgc gtgccacgaa
    10201 tggaaacgtg acaaagactg tggcgagcac gatacccggt acggcaaaaa tgatacgtat
    10261 gccagcttcc tgcagcgttc cacccagcca accctgcagg ccaaagacca gcacgtaaat
    10321 aaggcctgag atgaccggcg aaatcgcgaa cggcaggtcg atcagcgaga cgagcaggct
    10381 cttgccgcgg aaatcgaact tggcgatggc atatgcggca accacaccaa aaacaaggtt
    10441 cagcggcaca gaaattgcag cggcaaccag tgtgagtttt atggaagcca gcgttaccgg
    10501 gtcagagaga gcttcgagat aaaaatccag ccccttttgg aatgcctgcg taaaaatttc
    10561 atagagcgga agccccagca gcaaggtcag gaataccacc gcaaacgcaa tgaacgcagc
    10621 cttgagccag agaggatctg tgaggtaact gcgctggccg ggcgtcatac tttcctcttt
    10681 tggcgaatct gtacgtaatt gagcagcagc aacagcgaaa atgaaatcag cagcatcatc
    10741 gcagccactg ccgttgcgcc ctgcacgttg tgctgctcca gctttgtaat tatgattaac
    10801 ggggctattt cgctcttgaa cggtatgttg cccgcgataa agatgacgct cccatattca
    10861 cccagggccc gtgaaaaggc catgatggta ccggaaaaaa gcgcaggggc tactgaaggc
    10921 agaatcacac gagcaatggt ctgcaagcga ctcgcgccca ggctcatggc cgcctcttca
    10981 atttcttttt caaaatcttc gagcaccggc tgaaccgaac gaacgacgaa gggcaggcca
    11041 ataaagacga gtgcgatcgt gattccggta ggattgtaag cggctttgat accagcgcgc
    11101 ccgagcagcg agcccaggat accgttctcg gcatagactg tgcagagggc aatacctgca
    11161 acggcggttg gcagtgcaaa gggtaaatcg accatagtgt cgagcaggcg cctgccccag
    11221 aaatcgtagc gcgtcagaac ccaggcaata ataaatcctg caaccatatt gatcagcgca
    11281 gcgacaaagg cagtcgtaaa actcagacgc agggacagca aagtccgctc agccagagcg
    11341 tgttcggcaa attcatgcca gccgagagaa aagctcttcg cgaacatcgc tcccagggga
    11401 atcaggacaa taacgagcag atagataacg gtataggtaa gagtcgcccc aaaccccggt
    11461 agtatggatt tttcccggaa actaaaacgc aagacagact cacttgccat agatctggtc
    11521 aaagatagcg ccatccgcga agtgcgtctt attcgccttg tcccaaccgc caaaagcgtc
    11581 ctcaatcgtc acgagagcga gcggccggat gtgcgcatac ttttttacga tctcctgcct
    11641 gcgcgggcgg aagtaatgtt ttccggcgag atcctggcct tcgtcactgt agaggtattg
    11701 caggtaagcc gacgcgagct cgaggttacc ttttttgcgc gccacggatt ccacaacggc
    11761 gacactgggt tcggcgagta tactcagcga cggcactaca acttcgaact gattgggaaa
    11821 ttctttgaga gctaggtagg cttcgttctc ccaggcgatg agcacatccc ccagtttacg
    11881 ctgggtgaaa ctggtcgttg cgccgcgggc accggtgtcg agcacgacca cgttcttgta
    11941 gaggctcttc acgaatgcac tgatttgatt tttgttgccg gcaaatttct tttctgcgta
    12001 ggcccacgca gcaaggtagt tccagcgcgc gccgcccgaa gtcttcgggt tcggcgtgat
    12061 gacgcccaca ccgggtgctg ccaggtcaga ccaatcttta atacctttgg gatttcccga
    12121 gcggaccacg aacacgatgg ttgaggtata aggtgcactg ctctgcggca gcttcttttg
    12181 ccagtcagag ggcagaagtt ttgcctgctt tgcaatagtg tctatatcag acgacagcgc
    12241 cagcgtcacc acatcggcct caaggccgtc gatgacactg cgtgcctgct taccgctgcc
    12301 accatgcgac tggcgaaaac tgatggtctc accggggtgt gacttatacc actcgcgcgc
    12361 aaaggcttca ttaaactcac ggtagaattc gcgggtggga tcatacgaaa cgttcagcag
    12421 ctctcgcccc tgcttcttcg gaccacaggc ggccgcggcg accataagga gcaacagact
    12481 ttttttcatt ataacgaccg acaactccag agcgcaggat atatcgtcaa tgcgaagcgc
    12541 cggctaccgg cgcttcgttt cggcttattt gccatagatc tggtcgaatg ttcccccgtc
    12601 gtcgaaatgc gtcttttgcg cgtttttcca gccgccaaat gccccgtcta cagtgatgag
    12661 cttcagcttt ttgaagtggc tgaaacgcgc tgccgctttc gggttgcgcg gccggtaata
    12721 atgttttgca gcgatgttct gcccctcagg cgattcaaga taatcgaggt aagctttggc
    12781 gatttcagca ttccccttgc ggttggcgtt ggcatcgacg acggacaccg aaggctccgc
    12841 cagaatactc agcgtcggta cgatgatttc ataaccgtcg gccgcgaatt ctttgctcac
    12901 caggaaggcc tcgttctccc acgaaatata gacatcacca atcttgcgtt gagtgaacgt
    12961 cgtagtcgaa ccacgtgcgc cggtatcaag caccgcgaca ttcctgtaca gcgccttgat
    13021 aaacgtccgc gggtccagct tcttttcctg cgcaaatgcc cacgccgcca ggtaattcca
    13081 gcgggcaccg cctgaagttt tgggattcgg ggtgatcaca gcggtaccgg gcttcaccag
    13141 gtcatcccaa tctttgattc ctttgggatt tcccttgcgc accacaaaaa caatcgtcga
    13201 ggtgtaagga gcgctgttgt tgggccggcg cttctgccag tcggtgggca gcaacttagc
    13261 cttttcggca atcacgtcga catcgccagc gagcgcgagc gtcacgacgt cggcgtcgag
    13321 tccatcaatg actgagcgcg cctgcttacc tgacccgccg tgcgactgct tgaaacggat
    13381 gttgtcactg gggttcttct gtttccagtg gcggagaaag gccgcgttga actcgcggga
    13441 cagctcgcgt gtcgggtcat aagagacgtt aaacagttca taatctttcg cgatgattgc
    13501 cgtcgcgctt gcgaagagca gcacgatggt cttaaaagca gattttttca ttttattctc
    13561 cttctgtgat atgtggtttt tgagttcaat cgaacaatag cgctgaaagt tccaggtacg
    13621 cgaaatggga atcccctggg cgtttgaatt tctcagtgta gctgcctgcc ctgaaatagg
    13681 cataaccgat gttgaccttg aggtgaggaa acgggtagcg tatgcgtatg ttgtattcct
    13741 cgccaaactt gccgtcggta ttcgcattgc cagcaggggt cgcggtattc aggatgcccg
    13801 cattgttcca gcgatcagtc gggctcgcag cccagtacca gacgtaggcc gtgtcgatct
    13861 tgagtttttc gattggttcg aattcgagta tcacctttgg ggtttccagg ttctccagct
    13921 ggaaataatc attggccgac catggccgcg caaacccgta caagcgctca aagcgattct
    13981 gcgtgttgtc attgcgatcg atatcgcctg aaatatacgc gtaaaatccg gcgaggcgcg
    14041 gtttccacgg atgtttaaac gtgtagccga cttctgcggt cgtgttgaaa gcatcctgct
    14101 tgcgttcgac tgcgttattt ccgagctggt ctaccccaaa ttgcttcgta taagacagat
    14161 cgtaatcgaa acccgtctta ccgagcgctg cgtatgcccg accacccacg gtgtgaatgt
    14221 ttctgaagaa gcgggcgcct gcatcgctcg tcgggtacca atcggagcgc gctggttggt
    14281 tgtcctgatt gagcccaaaa tagtacggct ggaaagtaac ccactgcgac cattcgcgta
    14341 cgctgagcac tccaccatag aaccattgct ggccaatcgg ctggtcaagc ttctcaatct
    14401 cgcgcacgag cggctgcgca gctatcaggt tgagatccca gttattttcc tttctaccga
    14461 tggatatcca gccgccctgg aatgtattgg tggtattgcg ccattcgttg agaccaacaa
    14521 gcctgcggtc gatcagctcg agcgcatagc ggccgccacg aacggcgagc ggacggttat
    14581 aaccgaagag attacgaaag tgcacctcgg cataagcctg gataggttca aactcattga
    14641 aatcgcgggt atctgcgccg gtcttaattg gtgtccgccg tgaatcctgg acttctgtga
    14701 cgagacggaa ccagtcgaac tcaagcttga gaaatgcccg cgttttaaaa agcagcggag
    14761 tttcctgcgt cgcaggatta ccggacgggc cctgtggatt caccggggaa tttggtctgc
    14821 ggaagtcatt ctctctgaac tcaccgcgcg tgcgctgttc gacaccgaag ataatccagt
    14881 cgatattcct gaatacacca attccggttt cactcagccg tttcgcataa gcaggctggg
    14941 tgctatctat gggttcggtc ccgtaacttg gcgagcgcac ataatagcta tcgcgaactt
    15001 cgaccgtagc cgctgtcttg tcggcttcgc tcacctcggg tggaattgct tcttcgtcgc
    15061 tcagtgtctc tgcctgctgc acggcagcct ttggcttttt tgcctgcgct ctgagcgccg
    15121 caccttgcag cgaaacaaca agcgctgcaa gataaaccag aaatcttttt attttcattt
    15181 tcatctcctt cgtccccgcg ctgctgcgca gggacatact cttcttttac ttacagaagg
    15241 ggatgtccgc atgcagcaga tgttgtgctt gcatggcaca acaacagagt tgcatgcggt
    15301 aagagatgat aaatgaaggc aaagctgcgg cagatccggc gttcattgtg ggatttcctt
    15361 cggtcacggc agctttcatt gtgatcaggc ttctttttta gccaaacaat ccttcaatcg
    15421 acaggtagcg ttcgccggta tcgtagttga acgtcagaac gcgtgcacct ttcttaagct
    15481 cgggcagctt tttagcgaca gctgccaggg acgcgccgct ggaaataccg gcaaagagcg
    15541 cttcttcacg cgctgcacgc ttggcgtact caaaggcttc ttctttcgtg acctgtatta
    15601 caccggtcag aatctccttg cgcaggttct taggaataaa tccggcgcca ataccctgga
    15661 tcgggtgagg cgccggcgaa ccgcctgaaa taacaggaga agcagcgggt tcaacggcga
    15721 agatctgcag gttcggccac ttctgccgca gcacctcacc tacgccggta atatgaccac
    15781 cagtacccac accagtgatc aagacgtcga taccctcggg gaaatcccgg agtatttcct
    15841 gcgcggtgaa cttggcgtgc gcagtgatat ttgcttcatt ctcaaactgc tgtggaaccc
    15901 atgaaccagg tgtttgtgct gctagctcgt tcgcacgctc gatcgcgcct ttcatgccct
    15961 tttcgcgcgg cgtcaggtca aactgcgctc cgtatgaggc catgattgcg cggcgctcta
    16021 ccgacatact ctctggcatg accaggatga tcttgtatcc ttttgcagcc gctacgagcg
    16081 agagcccaat acccgtgttg cccgacgtgg gttcaataat gacgctgcca ggcttgagga
    16141 ttcccctgcg ttccgcgtct tcgaccatcg acagcgcgat gcggtcctta atactaccgc
    16201 ccgggttttg gcgctcgagt ttgacccaga cctcgtgttc cgcggcaaag agccggccta
    16261 gttttacatg gggtgtattg ccgataagtt cgagaatgct gtgtaccttc ataagttctc
    16321 cgctgtcatt cgttcgctat agcgaacgaa tgacccagaa cgcgaattgc cgccacggcg
    16381 tcaagcacaa catacaaaaa ttcattataa cagaccgcct gccagaagca ggccctggca
    16441 actcctgcgc cagtaacccc attgccgaat agctgagcat ggctccgctt tttactgtgc
    16501 tgcgcgattg cgttttcggt agcttccatt caatgcctgc aagaaagtca cgcgctgcct
    16561 cagaaaaacc cgctggcggc aagtcccggg cgcagaccgg cgaaaacctc accggcgttg
    16621 ttgcggataa cgtcaagcgc ctgcgctccg accttgatct ttcgctcgat aaactggcaa
    16681 acctgtccgg cgtgagtaag gcgatgctca gccagatcga gcagtcgcgc agcgcgcctt
    16741 caattaacgt tctatggaaa atcgctcgcg cgctcgacgt acctttcgcg gcgctcatca
    16801 gcaagcgcac cgacgcgcag ctgcaggttg tcaggcgcaa cgagatgaaa attctcagct
    16861 cgcaaaaagg cggttttgtt tcgcgagcct tattcccact cgacattccg cggcgcgtgg
    16921 aattttatga actcgagatg tcacagggct gcgtcgaaaa agccgtggcg catccacctg
    16981 gcactgccga gaatattgtg gttgccgaag gacagataga aattgaaatt aatgggcaga
    17041 aaaacctgct cgaacagggc gactccatct actttgtcgc cgaccagccc cacgtttacc
    17101 gcaacctggc aaaaggcact tcccgtgcat acctggtgat gacctacgca gagaagcggg
    17161 tttaaacagt aggacgaaaa tcctttacac agctgtccgg cctctgcctg ctccagtggc
    17221 ggcagtccca tgcagatatt taatttccag ggctactcac ttgaggcaat actgcttgct
    17281 gcagcgctgc tcgttacctt atctgtaatc gccagccgta tctcttcaaa gtttggcata
    17341 ccattgctgc tggtgtttct cggcatcgga atgctcgctg gttctgacgg ccctggcggc
    17401 attgcattcg agaattaccg gatggcattc gccgtcggct cagcctgcct tgcactgatc
    17461 gtgttcgatg gtggtatgag cacgtcctgg aacagcgtgc gaccaattct gcggcagggc
    17521 atcagcttgt ctgttctggg cacttttctc acaggggcag cgactgctgc cttcgcccat
    17581 ttcgtactgc atatgcactg gtcagattcg gtcctgctgg gagcgatcgt ttcttccacc
    17641 gatgccgccg cggtgttcag cctgctgcgt gcccgcagcc tcggcctcaa gggcactgta
    17701 aaacaaacgc ttgaatttga ggcggccagc aacgacccga tggccatctt tctgacagtc
    17761 ggtgttcttc tttttttgag taatcccggc gccagtatcg tcgactttct gaaactgctc
    17821 gttatgcagg gcggcatcgg tctcgcatta ggcctcctcg gcggtcgggc catacggtgg
    17881 ctcatcaata atgccggtat cgagtacgaa ggtctctacg gcgtgctggc tctcggtctg
    17941 atcgtcggtc ttttttcact gacgggtctc attggcggca gtggttttct cgcagtttat
    18001 gttgccggaa ttattcttgg caactatgag atgctccaca aagcaagtat cgcccgcttc
    18061 ctggacggtg tagcatggat cgcacagatc ctcgtgttcc tgaccctcgg gttgctgtcg
    18121 tttcccacgc gggtgtttcc agtctggcag gagggactgc tgctggcggt cttcatgatg
    18181 ttcgtcgcac ggcctcttgc tgttttcctg gctgtaccgg ggcgaggcat gacactgcgg
    18241 gaacgcacct ttgtttcctg ggttggatta aggggagcgg caccgatcat actcgccacg
    18301 tacccgtgga gtatccattt tccccgtgcc gaatatctat ttgatcttgt attcttcgtg
    18361 gtgatttctt ctgtaattgc gcaaggcatc agcatcccgt ggctggcaaa gaaactcggc
    18421 attaccgtgc cactggctaa accccgccgc attgacctcg cgcacggtgt tttgccgcag
    18481 gggttcctga gtattgagat ccatgtaacc gacgatgcgg cggcaacagg gcagaaagtg
    18541 gttgatctcg agctgccatc gggcgttatt ctgaccagta ttgagcggag tgatcgtttc
    18601 ctgattccgc gtggcgacac acttattgaa gccggggacc gtatctttgg cctcgcacgc
    18661 cagaccaacc tgccggcgct gcaggacatc ttcggcaaga cggaagttgt agcctgatag
    18721 gccgcgccga aagttaaatc tctaccgggg aaacaaatcc ttctggcttc agcgccagca
    18781 gcgagcagtt cagcttccgc acaatattct ccgcagtgtt gccgaagaga aaaccaggga
    18841 tgcccgtacg cgccactgtg cccatcacca ggatgtcaat cttatgctcg gtgcaaaaat
    18901 gcggaatgag ctcacctgcg cggccgcgcg ggtggtgaac gttttggcgg ccgctgatac
    18961 cggctttttg cacgagttcg ccgagttttg cgagatgcag gtcaccggcg cgcagcaccg
    19021 acgaagaaac atcttctgcc ggcagcgaaa tccaggaatt gcggcgcagg aagccctcca
    19081 gctcgtactc ccagcacgaa acgatacgca gttcgccgtc gcactggtcg gcaaggctgc
    19141 gagacagctg cagcaggcgg agcgtcagct gcagtgccgg ttctgcactg gcctcggcat
    19201 cgaccgccac cgcaacgcgg atattctcac ggtggtgggc gatcggcctg ctcagccata
    19261 cggcacccgg gcaatatttt aacagcgcca tgtctacagg ttcgaacccc tgcccgctct
    19321 cggtgctgtc ggcttctttt atgaggagct caaaatgacc ccgcaacata tgctgtatga
    19381 tgagcttcgc gggttcatca cttgttacga tttcaactgt tacctgcagg tcgccgcaag
    19441 taagttcgtg agctacttcg gccagtgccg ctgctgtgcg cgatttcagg tacgcgtcgt
    19501 agccagaacc atgctgcgaa aggctttcgg gcaactgcgg tcggacgacc agcgcctgca
    19561 ggtgcgcgcc gttattacgc gcaaggctga ctgcctgccg catcgccggg cggtcatcgc
    19621 ccgcacaggc ggtagcataa aggatgcgcg taaatttctg caaagcgacc tcttaattct
    19681 aacccttgcg ctcttcgagc caggcgaaga accaggtgcc ggcaaaaata ccggtaacaa
    19741 taaacactcc ggacataagg ccataaccca ggatcgcgag cgcgggtccc gggcaggcgc
    19801 cggttaatgc ccagccagcg cccgatatca ggccgccaat tacatgcttg cgcgacagtt
    19861 tctcaacgct ccagtcggtc tcttccgcca gcagcggttt gcgtccccac tttttccaca
    19921 gctgcacgca gatgaatgaa acaggaactg caacaccaag cacgccatac atgtggaatg
    19981 actgcagcag gaacatttca cgtatcacgt cataactcga aacacctgcg cgaatcagga
    20041 tatagccgaa cagcgtaccg agcaaaagaa aaaccagtct gaggccaaga gggccattgt
    20101 tagaagttgc cattatttta cccccgaaac ggctgccagc aaaaacgtca cagcaacgcc
    20161 cgcgaccagg aaaccacaag tcgccaccag gctcgcgacg gagcccttcg agagaccaga
    20221 gatagagtta cccgatgtac aacccttggc catgcgagag ccatagcccc agaggaaacc
    20281 gccagcgatc aaaacgcctg ctttgacagc aaggttatcg ccccagatct ggtcgaactt
    20341 gccgagcgca aaggtcggtt gccagccagt ggtcgtgaaa gctgcgattg caccaccggc
    20401 cagcaggcca agagtaaaga aggtacgaaa gccaaaaggc ccacccagat caggacgctg
    20461 gaagaacttc attcgtgaaa aaattgagca cacactcgcg taaccgcggg tcactgaaag
    20521 aaagcggccc gtcaggatga ccagcgccac tgagacgccc gcaatcgcta atccccccag
    20581 ccaaaatggc cagatgtatc ctgctgtcat aaaacctcca caacaaccat ttgtatgcta
    20641 taacgaacag ctaaactgcc gcgtcaacgc aaaagctccc cctaccgtga ccggggggcc
    20701 caatgcggcg gcaagccgct ctggcaactg caattgtttt gtcctgtact atatcactga
    20761 attaccggct ttagtgcatg gcagaaaatt cacaggtgtc gcttattggc ggcgacccaa
    20821 ttcaaccagg attgaagctg aaaggagtgc tacgctcgct cgcactcgcc gttatcaagg
    20881 acgaacgcga tattgacctc ttaacctgca tgatcaagct gagcattctg ctaccaggct
    20941 cagccgctgc gattctgctg ctacccgaat accggttata cctgggaatt gcccacattg
    21001 tccttttttt gctctacctt ggaccatata cgctgatgct gcataacgtc tcacaccggc
    21061 ccttgttcca ttaccacctg cgctggctca atgtctggcc gcaggctctg cttgccttct
    21121 tcttcggcat gactccctac acgtatttca gccaccacct cggtatgcac cacccggaaa
    21181 acaacatgcc ggaagacctc agctcaacga tgaaatacca gcgtgacagc tttctcggtt
    21241 tcctgcatta ctatctttca tttgccgttc ttggcatctt cacgctcagt ctctacttca
    21301 tccggcacgg gcgctggcgt ttttttcgta tggctgtttt gggggagctt tcatggtacg
    21361 cagcagttgc ccttgcattt tggtatttgc ccgaaccgac gtttatcgtg ctcatactgc
    21421 cctggttgct gacccggttt ttgctgatgg ctggtaactg gacacagcat gctttcgtcg
    21481 atacgaccga cgggaataac ccgtatgtga acagcattac ctttatcgat tcgccgtaca
    21541 acaaacgctg tttcaatgac ggctaccaca tcggccacca cgtcaacatg aaccggcact
    21601 tcctggatat gccgcaggat ttcctggaca atctgtccac ctaccgcgag aacaaggcgg
    21661 tgatctttcg gaagcttgac tacttcatga tctggttcct gctaatgaca aaacagtacc
    21721 gcatactgag ccgttttttc gtgaatattt ccgccaaaga aatgtcacgc gacgaaatca
    21781 tcgcattcct caaaagtcgc ttgaaacccc tgcgcgctta gcagaacatg ggcgcaatgc
    21841 ctattctctt accgatcatt gcgcagctgg gccctgaagt ggaggccata cgcctcccgg
    21901 gtttcgattc cagcacttta ccctatctcg tttatttcct gctggcggtt attggcggcc
    21961 tgcttttcct gcaatggcgc ctgcgcaggc gtgccgaaaa gaatgccctc agtaaccggt
    22021 tcatgcagat tatgctgcac cgcaacctga caaaacggca gtaccgcgcg gtgaacctgt
    22081 ttttccagag cctgaaagaa gcagagcaga acgaaatact gctttcacag aaagcgctgg
    22141 cacaccacct gcaccagttt ttacgggtac acgagcagat accggccaat gaccgggtag
    22201 aaatttttga caagattctg ccgggtatga catcgcagat cgaaatcaaa accgtcgatg
    22261 acctgcggac cggcgaaccc tgcgctgtgg atatagatca tacatcgcac ctcgctacca
    22321 tactgaaaac aaaggataac caggtactcg ttagccttac agaaaagatt gtgctgccac
    22381 tgggccctgc taaactctat gcctatcgcc ccaatctcgg cgggtttctg ctttctggtg
    22441 ctattagcaa ggtgaatggc atgtcggcca tcttcaaaca agaagggcca attgaattcc
    22501 ggggtgacca gcacctgatg tcgatcgtga acgtggcgct caaactcgaa ccctggccga
    22561 agcctgaggt agaagcagat cttgagcccg gcgaaccgcc ggtagagtcc ccctacctgt
    22621 tagccgaaac cgaacggctt tctgatcggg cgctagccat ccgctttctc gaaacaccac
    22681 ccgcgttaat tctcaagcgc caggaattct gggagatgac gcttgaatta ccgagtgaac
    22741 cgctggtttg tcgcgtgcgc gtaactcctt ataaaccgcc tgaatactgg ctggtgcgac
    22801 cagttgacat ggacgatctt ggccgtcgca ggctttaccg tttcatttct gagaacaaac
    22861 ccgtccgcga acacttctag cgcaggaacc tgctagcctg cgcggatatg ccaggctttg
    22921 ggacgggtga atgcttcaat gccgtaggtc gataatgtca gccccacgcc gctgttacgg
    22981 cgtccggacc acggcaggcg cgggctcacc cggtcacagc agttgatgta agcgctgccg
    23041 gcattcactc ctctcagaat gccccgcgcg cggttctcgt cgcggcagta gactgccgct
    23101 gtaagcccgt attcggtatc attcatgagc tcctgtgctt cggcatcgtc tttgaccttc
    23161 atgatgccga ttaccggacc gaaactttct tcacgcatca gcgccatcga gtggtcagca
    23221 tttgcgataa ccgtcggctc caggaaaaaa ccaccgccct gtgccacaag ttttccgccg
    23281 cagacgagct taccgccttt cgttacggca tcatcgatct ggctcttgag aaagtcatac
    23341 gcgggttttc cccgggtcag cgcaccgata taggtctctg cctgctcagg gtcgccgagg
    23401 cgaaaactgc gtacggcctg ttcaaagtgc cgcatgaact cctcgtagat cttttgatgc
    23461 acgtagatac gttcgacact gcagcaactc tgccctgtat tgtacatcgc gccgtctgcg
    23521 gtcgcttctg cagcggcttt cacgtcaaca tcgtcggtaa tataggccgg gtctttgcca
    23581 cccagctcga gttgtacctt gatgaatttt ttcgcagctg cttcagctat tttgcggccc
    23641 gttgcgactg agccggtaaa gaatatgcca tcgatcggtt gctcgagcag tatcgcgccg
    23701 gcgtcacccc ggcccacgac gaccgcgaaa atatcttctg gtacacccgc ttcgtgcagg
    23761 taccgcgcca tgagaagccc cgattgcagc gcaaactcgg acggtttata gagaaccgcg
    23821 ttgcctgtca gcagcgccgg tacatataca ttggatccca caaaccacgg ataattccag
    23881 gcggagatat tgccgatcac gccgagcggt tcgtgctcga tttcttcgac gagcgcgtga
    23941 ccatcgcgca acaccgtctg cgcaccaaca gtcttttcag tctcggccaa aaagaaatcg
    24001 aagcgcgcaa gtaagcccct gagctcattg cgcgactgcg cgatcggctt acccacatca
    24061 tgcgtcagaa gcagtgcagc ttcttcgatg tccttttcca cgcgatcgcg gaagcggcgg
    24121 atgatttcca gtcgctttgc caggccaagg ttctgccacg ccggctgcgc ggtgcgcgcg
    24181 gcttcatatt ttcgtgcaat gctcgcgtgg tcgtctgccg gtactttgcc caaagatttg
    24241 ccattggcgg ggttgataat ttcaatttcc atatttcctc acctgcgctg catgcgcaat
    24301 tccgttcttc gcacttcgca ttcctgcata aagtctttta gcagcgggtc acccgacagc
    24361 agcgcattat cttcatcatc gtgaaattcg ggatgccact gcactccgca gatatacgga
    24421 gccccttggt attttatacc ttcgacaatc ttatcttcgg tagaaatcgc ttccactgcc
    24481 aacccttcac cgatatcctt cagcgcctgg tggtgcacgc tgttggtgcg aaagctgcca
    24541 cgaccgaaaa ccttttccag gtaagacccg tgcttgaatt caatctcgtg aaaattctga
    24601 tcgtagacat tccagtcacg gtgtaccagt gatttgtcga tctgcgtgtt gatatcctgg
    24661 tagagcgatc caccgagtgc gacgttgatg agctgcgcgc cgcggcagat gccgagtacg
    24721 ggcttttcgg cggccttaaa agccttgaag agttcaattt catagagatc gcggacgcga
    24781 tcgcccttcc attcgtcttt gagcggcttc tcgccgtaac tctccggggc aacatcagct
    24841 ccgccgtgta gcaccagccc gtcgagcgac tctgcgtact cagccagttg catctttccg
    24901 cctggggcga ctgacggaat catgaaggcc tgtgccccaa ggcgcattac ccagtgagca
    24961 agagactctt cgacgtaaag cagcgttttt cctttgaata ttgcccgcgt ggggtctttg
    25021 tgcatgaagc aggcagaaat gccgattttt aatctttcca taaggcgtct cgcgcgacag
    25081 actgcgttcg ctccatacgc cttatagtct ttttgcttct gccgctaagc tggcagaagg
    25141 ccggatcagc cgcgcgacaa agccgcgccg acaagcgatg cctgcatggc agcattctta
    25201 gcctgtatga aacataccgg gcaaaggaat gccaggtctg aggtgcaatc gtcaaaatcg
    25261 gcatcgcccg caacccgggc aacgctcggc gatacctttt cgccacacga gctgcattca
    25321 atttcttcaa caacctggta gttcatgctc tgcttttagc acagaaatga agcctggtca
    25381 atccgcaaca atcggcggcg gaatgcggaa cattacctat gggtccggaa aactgaccta
    25441 taggaggtca gtttagccat ggcccccctc tcaatgcaaa agcatgccgt tagtgcacga
    25501 acatcaagtc acagcacttg ccggaatata tcgctgaagt ctttctcgct caccgcaacg
    25561 gggttcgagg gatggcagcc atcggccact gccacgcgta cgagggcatc caatttatcc
    25621 gctgctacgc cagcgtcttt caaagtggct ggaatcttca gctcggcctt taggtgcgcg
    25681 agccagccca ggaactccga ccccttagcc agccccaccg cctgcgcgag acggtcaaac
    25741 ctgtcgggca cagccttggc attgaatgcc agcgcatggt cgagcatgat gccgttggcc
    25801 aggccatggt gcatgtcgac aattgccgag agcgcgtgcg cgcacgaatg caccacgcca
    25861 agtccctttt ggaaagccac agcacccatc atggatgcca tcagcatctc gccgcgcgcc
    25921 ccgagatcgc cgccgttttt gaatgcacgc ggcagcgcac cggccgcaag ccgcagaccc
    25981 tcgagcgcga taccgtctgc catggggtgc cagcctttcg cgagaaaagc ttcaatcagg
    26041 tgcgtcattg catcgaggcc cgtcgcggcg gtaacgtgcg gcggcatggc agaagtcatt
    26101 tcaggatcag caaagactgc gcgagccagc aggcggggtg aaaagatcac tttcttcaca
    26161 tgagtttcat cgtcagcgac aactgtacta cgcccgactt ctgaacctgt gcccgaagtt
    26221 gttggaatcg caatccagta agggatttcc tggtcgaacg gccgcgcgcc gggcttttcg
    26281 tcttcgtag
//
LOCUS       DAOQJF010000003        25048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_876_length_25048_cov_5.206458, whole genome shotgun sequence.
ACCESSION   DAOQJF010000003 DAOQJF010000000
VERSION     DAOQJF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 25048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25048
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_876_length_25048_cov_5.206458"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1017)
                     /gene="dctP"
                     /locus_tag="PLF85_00310"
     CDS             complement(16..1017)
                     /gene="dctP"
                     /locus_tag="PLF85_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNE18121.1"
                     /translation="MLSTSSFVRVFFATLLLLTLTPLSAEITLKYATLAPENTIWAKV
                     IKKCVDEISAKTNGEVKVRVFYGGIAGDEPSVMRKLKTGTIDIASFTGQTLGTVVPES
                     RLFELPFFFDNFRQLDAVVAAVYPDLSKKFLAQNLVLAGWGENGFVYLMSKHKPIVKV
                     ADMQGVKIWAPKGDRLVLAMLSEYGLVPVFLGFEAVLPQLKTGGIDAFYAPPYGAIGL
                     QWFREAKYLTNARLANAAGATLLAKSAYDKLTDAQKTIVHTTLEKYARELTLELRREN
                     EKAMQVLLGKGIEKVEPNAEELAQLKRTALLVQDKLAGELYSKELLQKARKARDSVK"
     gene            complement(1041..3224)
                     /locus_tag="PLF85_00315"
     CDS             complement(1041..3224)
                     /locus_tag="PLF85_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE18122.1"
                     /translation="MLRFIHLLFLSLIFLFDAAWADEQPAIVYGADEAFRPYEWFSDE
                     GNLEGFQIELIRAIGEAQGRRISFVTGPWSKIRQSLLDGSVQVVSMFDQPVRRIYADF
                     SKPHSVLASEIFVRIGSKPILSVQELAGKEVIQQEGALSAEYLRTLGIRYTSIQVKDQ
                     FDAMRLLATGKHDCVITTQVGGRYALQKLGIRNVITTGQPVLASNVAFAVRKGNSNLL
                     EWLNSGLDRVKANGTYDVLYQKWFGESNRVRMSDLQAMRNVFVGIGLAIVVLVAVIGL
                     WIKMLRRAVSTKTFELQYLADHDSLTGLYNRHYITNAIHSAIADFHLHSGQRNFGILF
                     IDLDRFKLVNDSLGHHVGDLLIKAVGFELLASVEDRCRVARWGGDEFIVLVNPVGDGA
                     ALQRIAMRIAEKLRTPYNFKGKDIFIGASIGAVMSSLRYRTAEEMLRDADNAMYRAKS
                     DRRVSVRLFEPQMHDVAVRNLKLSSDFQKGVARGELRNHYQPIYSLATGKLSGFEALV
                     RWQHPELGLLSPAVFLPILEKTEAMRQLGEWVLQNAIETAKRWHHHYAADFYISVNIS
                     DEQFAFGNLCDTLSGMLEKFRLPPRFLRLEITETVLMADVVGGMSQLEELRQMQVRIL
                     IDDFGTGFSSLSHLASIPAETIKIDRSFVHGLVHGSRNYEIVRAMLALTRNLHLEAVA
                     EGIETRHERDLLAEMGCEYAQGFFYSEPLIEMAAEELLKKITVQP"
     gene            complement(3324..3941)
                     /locus_tag="PLF85_00320"
     CDS             complement(3324..3941)
                     /locus_tag="PLF85_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNE18123.1"
                     /translation="MKKSTFIYAAALSVMAALPSATMAVGFEAKAGVSSVRAANDKAA
                     VDAAIALDMRLERFFAFVPELNVQALGFDSPSGTPVVVNGINQYPTVSKTYYTFIPMA
                     NMRLYIPMGSEDTPAVQPYITAGLGFGYSMYNQKNPDFTDSASGFMYQGMLGAMFNLG
                     MLTEGSASATNLMLEAGYRGGQMEKSGAKADWNGYVIRAGVNFQF"
     gene            complement(4054..4644)
                     /locus_tag="PLF85_00325"
     CDS             complement(4054..4644)
                     /locus_tag="PLF85_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNE18124.1"
                     /translation="MQRNEDRLVVIGASAGGPQALEVILRLLESESLPVLIAQHMPEN
                     FTRAFARRMDEVARVSVCEAGHGMVLRTGEVYIAPGGRHMRVCREAHELRICLGDDAP
                     VNRHKPSVDVLFNSVIEVKPRAALAVLLTGMGNDGAQGLKDLRDSGYPTLIQDEATSS
                     VWGMPGAAAKLGAARAADIRPLEAIAAAVRLFALQK"
     gene            complement(4659..5198)
                     /locus_tag="PLF85_00330"
     CDS             complement(4659..5198)
                     /locus_tag="PLF85_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNE18125.1"
                     /translation="MRRNRSEFHALPEGGREIYLQPGDWFWGENRVRIKTLLGSCVAI
                     CVWHPRLKVGGMSHCLLPSRGLLVPKGYEADYVEDSELSGRYVDEVLEIFFREMLRAG
                     TRPQDFVVKVFGGGKMFEFVDKTGITVGERNLQMMRAILAREGMAIAAQHVGGTGHRH
                     VIFELWNGDCWVRHEELPQ"
     gene            complement(5185..6012)
                     /locus_tag="PLF85_00335"
     CDS             complement(5185..6012)
                     /locus_tag="PLF85_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNE18126.1"
                     /translation="MNRMPPVAGSIELTDAEFAIFQKLIYDWAGIHMPDSKKALVSSR
                     IMKRLRHYGLNSYTDYLRIVRSEDDRGERQMMINLLTTNETYFFREIRHFDWLRERVT
                     AHDRSEPFRVWSAACSSGQEVYSIAMTVADVLGETGWQVIGTDISEHILSQALAATYP
                     LEMAEEIPQHLLKKYCLKGAGSEQGKFCIAPALLKNIRLQRMNLLEHRPGSFGYFDVI
                     FLRNVMIYFDRETRKKVSEALAQSLKPGGFLVIGHAESLHGISDHLKAVKPTIYAPQ"
     gene            complement(6009..6542)
                     /locus_tag="PLF85_00340"
     CDS             complement(6009..6542)
                     /locus_tag="PLF85_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18127.1"
                     /translation="MAGTRPGTSYDRFRPLPSEKDILLLRNAPLHVIEGTGAAGMLLG
                     ETETDLRARYGEPAYRTYSDPETLTYTEATFNAEFVMKNDRIREIHLEIEKHKSPSLE
                     FFTALGLHETQLRGLNSEQAGRFLGEFYRSRRWRVVGNEVDVYGRGIRFRFRGNRLIR
                     LAVYEPQAYSAAGEDIP"
     gene            6574..7386
                     /locus_tag="PLF85_00345"
     CDS             6574..7386
                     /locus_tag="PLF85_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNE18128.1"
                     /translation="MPIVQHMTKKRALFKAYEDPEFMTSFDARPLRIQAEYLMPLTHF
                     EKLRVKDTIVFFGSARLPAPHQAKEEREKLQAAGEWNDVEAAHFNLRLRYYKDAVRLA
                     YKLTQWSKSLPAEARRFLICSGGGPGIMEAANRGAAHARGLSVGMNISLPFEQEPNHY
                     ITQTLNFEFHYFFMRKFWLMNLAKALVIFPGGYGTLDELFEVLTLVQTRKVQKRLPIV
                     IFGKEYWDQVINFEAMARWRMIKWEDIKLVHFSDEPQDAFEYLTKNLTELYL"
     gene            complement(7418..8500)
                     /locus_tag="PLF85_00350"
     CDS             complement(7418..8500)
                     /locus_tag="PLF85_00350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNE18129.1"
                     /translation="MARQADKWLAGYFREHKSYGKRDRRWYSEEFFRRLRTVIGYLPE
                     EAASDAASAWRALASVDWARSTQSTGADTLPRTWKKWLEHSSIRQAKIPQFLEQLATK
                     PPLYLRANYIDRVPEIEVELRAAGFSVETLRHDERWVTFRVEGELPVYELAALKKGWL
                     EIQDLASQHLGAAVDAHPGMAVWDVCAGGGGKTMQIASQMQNRGVLYASDIRDYKLDE
                     LKLRARRAGFHNIRRFVYDAETQVEPTREVTNAGGFHRILVDAPCSSSGTLRRNPDVR
                     FRIMPDDPAKFAALQLKILSAVQGYLRPGGRLVYSTCSVFKTENEDVVQSFIAQHPGF
                     KIVESRWVGSPDLDSDTMFYSILDRL"
     gene            complement(8611..10533)
                     /gene="htpG"
                     /locus_tag="PLF85_00355"
     CDS             complement(8611..10533)
                     /gene="htpG"
                     /locus_tag="PLF85_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNE18130.1"
                     /translation="MSTTQTFEFQAETRQLMDLMIHSIYSHKEIFLRELISNASDAID
                     KRKFLGLTDKSVTQETNNFIRIEVDKTARRLTITDTGIGMSRDEVVQNIGTIARSGTK
                     ELIEKAKASKDAVDLIGQFGVGFYSAFMVADKITMITRRANEESATRWVSTGAGTYTI
                     EDDSRAMPGTTITLDLKPADKENGLADFCDEFEIESVVKKYSDFITYPILMKKTIYKT
                     VGEGQDAKTEESIEEATLNSMKPIWTRSKSDVKDEEYNEFYKHISHDWEDPLKVISYR
                     AEGRIEYVALLYLPKRAPFDLYYTSFKGGLQLYVKKVLILERAEELLPRYLRFVRGVI
                     ESADLPLNISREILQQDRHITAIRKGLTTKIISTLTDLLEKEREKYADFYREFGPAIK
                     EGASTDFENKDKLAGLLLFASSADKEKTTTLQEYIDRMKAEQKEIYYLSGESRLTLEN
                     SPHTESLRAKGYEILYLVDAYDEIVLQNIGEFNGKKFKSASKGELDLADEKQKEKDSE
                     KVKEAEKAMGALLGCLKENLKDQVKDVKFSSVLVSAPSRVVGEDFDMSPYLEKILARN
                     GEKVAPRLKTLEINPDHDLVKKLNARYEANPGDPLLADYAQLLWGHAVIADGGEIKDV
                     ARFNKSLLAVASGALQ"
     gene            10603..12159
                     /locus_tag="PLF85_00360"
     CDS             10603..12159
                     /locus_tag="PLF85_00360"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE18131.1"
                     /translation="MQRNAVTVRFGLQFKLTLLIVALITAVFVAMGIYVAREKKNILT
                     ETASATAKKELASLAFIASRAILDRDDLALADSLQNAQGLPGFTIGAVYVPPKKNSLI
                     NRTGKELGEKQKEVERDGIETVRAHWDTIVASPVALGHRIEKAFLGDKDINVQYFHRP
                     VFHPFVKVNPPLLGVAQVAVTDEYINKSIRASLIELLIVGGIFFIAALFAAYLLSRFI
                     VKPVKVLSKGAAEVGKGNLDVTLPPLGSDELGQLAKQFNEMTASLKKASEEAKEQAVF
                     NDQVRQAKEIQEGMNPSRFLKKPEYQIKGFTRAAKGVGGDYYDFQMLGDGRIAVLISD
                     VSGKSISASLVMVLIKTVVSTYLKLFNTIRGDTIIRTINRVMCGEAHIDKFATIQFFI
                     FDPATKTLEFSNGGHGPLFLYRADRKICTASKLEGLPMGIEEENQYKLAKMQLNSGDI
                     VMLYTDGITECWNTAKEEFSLKRVREKLTEYANLNPKEIIENFVRDLDEFASGAEQHD
                     DMTMVVMKVL"
     gene            12170..12898
                     /gene="cmoA"
                     /locus_tag="PLF85_00365"
     CDS             12170..12898
                     /gene="cmoA"
                     /locus_tag="PLF85_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HNE18132.1"
                     /translation="MRDEIFKRPGSGKTPFEFNAQVAEVFDDMVSRSVPFYREVLRMT
                     AELAHDFYQPGTQIYDLGCSTGALAHALAEEFGNDTFSYNGLDNSEPMIARAKTHSPR
                     VNPGSTKIDFKVADIAAVNFEPASVFVSNYTFQFLKPLARQALLRRIYTALASDGCLL
                     LSEKCLEDSADVSRLYADHYHAMKARNGYSELEIAEKREALENVLIPFRVSENIEMLR
                     EAGFNPVSIFFKCYGFTSFLALKA"
     gene            12899..13840
                     /gene="cmoB"
                     /locus_tag="PLF85_00370"
     CDS             12899..13840
                     /gene="cmoB"
                     /locus_tag="PLF85_00370"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011650.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HNE18133.1"
                     /translation="MAGLQFSLPGYLPENSGALTEELARQEEKLATTGLTELLARIGA
                     LAGSGKTPAPGFSIQDGAVCSAQLLAGAEQIARDLIPWRKGPFELGGLQIDAEWRSDI
                     KWNRLKSHLPDMAGKTVADVGCNNGYYLYRIAAAGAKNILGLDPTLKYWLQYQLIAAH
                     TGQVADFLPLGWQVLWQMPRTFDTIFLMGVNYHDPDPLEMFHACSYALREGGALICES
                     VVVQADADLEIFPEGKYAGIGGVYAIPSPRALKRQLGFVGFQNVTLLYSDPLLPAEQR
                     RSEFSPQASLADHLREGDAWSKEGYPPPYRAALLCRV"
     gene            13878..14060
                     /locus_tag="PLF85_00375"
     CDS             13878..14060
                     /locus_tag="PLF85_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18134.1"
                     /translation="MKKILMALVAATTLLSVSVSAKTEKQIRDENKAKAYKEKKAAEK
                     AERQAKQEQKKAAKKK"
     gene            complement(14093..14809)
                     /locus_tag="PLF85_00380"
     CDS             complement(14093..14809)
                     /locus_tag="PLF85_00380"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNE18135.1"
                     /translation="MRNLLKSTPFLLLAIIGCSTGAKSARMDAGAAPAPAAEVSASTD
                     APERPQSTAPTPGQLLIYNASFTLEAKEAETRDLSGRIVDKAKGLGGYLSQRGSTSVT
                     VRVPSKEFYPMNDFIRSLAKVRYENATAQDVTMQYTDLKIRLDVQLKMLARYEELLKK
                     STSVKDAVEVERELSRITERVETLKGQIRYYDSQIGYSTISVSFQEPYSAFTKETKPG
                     PLGWVFYGAYVVIKWLFVWD"
     gene            15119..15931
                     /gene="rpsB"
                     /locus_tag="PLF85_00385"
     CDS             15119..15931
                     /gene="rpsB"
                     /locus_tag="PLF85_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNE18136.1"
                     /translation="MATISMKSLLEAGVHFGHQTRRWNPKMKKYIFTARNGIHILNLQ
                     KTIVLAKDAYEAMRDLASKGGKVLFVGTKKQAQADIEKAAKRCGQYYINNRWLGGLLT
                     NFRTVRQSIQRLRKLEEAFETNTVHELVKTKKEILQLDREIARLRKDLAGIRDMNDLP
                     DALFIVDPERESIAVKEANTLGIPIFAMVDTNCNPDLVQYPIPANDDAIRAVALFLEI
                     MANAIEEGKEGGEFAQVEIEEGESMDEHQLKSSMEKLDDLEEIESKYEEART"
     gene            15962..16552
                     /gene="tsf"
                     /locus_tag="PLF85_00390"
     CDS             15962..16552
                     /gene="tsf"
                     /locus_tag="PLF85_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNE18137.1"
                     /translation="MAIDQEKIKNLREMTSAGILDCKKVLEETNGDLEKAAGLLRERG
                     IVKAVKKMDREASEGAIVSYVHHNGKIGVLVQLNCETDFVARNEKFLELGRSIAMHIA
                     ATNPQFVKGDDVSPDVIEKEKEVQRAALIEEGKPADKVDKILEGKIKKFVSDICLLDQ
                     PFVKEPDKTVGDIIKASIAQLGENIAVGRFVRFALK"
     gene            16559..17332
                     /gene="pyrH"
                     /locus_tag="PLF85_00395"
     CDS             16559..17332
                     /gene="pyrH"
                     /locus_tag="PLF85_00395"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNE18138.1"
                     /translation="MDTPAERPAHITEGRAFRRVVLKLSGEVLAGEDKHGINVKIIRS
                     LAEEIRDIHREGVEMAIVIGGGNIFRGVKAASDGMDRAIGDYMGMMATVINGLALQDF
                     LEKEGLTTRLQTAIEIKSVAEPFIRRKAIRHLEKKRIVILSGGTGNPYFTTDTTAALR
                     AVELNADVIFKATKVDGVYDADPAKNPNAKRYRHLSFQDALVKQLKVMDSTAFSLCLD
                     NKMPIQVFDMNTEGNLKKAIQGKPIGTLIDAFSESRFYE"
     gene            17325..17888
                     /gene="frr"
                     /locus_tag="PLF85_00400"
     CDS             17325..17888
                     /gene="frr"
                     /locus_tag="PLF85_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNE18139.1"
                     /translation="MSEEANKIIADTKQRMQKTLEDLSHHMVQIRSGRATPSLVEDIR
                     VEVYGQNMPLNQVASITAPEPRLITVDVWDKSQMQAVEKAIQKSGKNLNPNNDGSMIR
                     ISLAEMTGETRKEMVKLAKAKCEDHKVAVRNIRRDANDNLKKLKGSSVSEDMIKGFSD
                     QVQKLTDDYIKKMDDLFASKEKDIMTV"
     gene            17924..18610
                     /gene="uppS"
                     /locus_tag="PLF85_00405"
     CDS             17924..18610
                     /gene="uppS"
                     /locus_tag="PLF85_00405"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNE18140.1"
                     /translation="MPKAPKHIAVIMDGNGRWAESRGLKRAEGHRAGTETVERLLDHL
                     IKVKVPFVSLYAFSTENWRRPRTEITAIFKLLDEFIIAKLPKMITQGVRIVTSGDISR
                     LPKRSRELLQDAVKKTARGRRLTANFCLNYGSRAEILRAAELALAASKNGKLTEKQLA
                     RALWQPSIPDVDLLVRTAGELRLSNFMLWQAAYAELYFTEKNWPDFAENDIDAAIAEF
                     GRRKRKFGGL"
     gene            complement(18616..19431)
                     /locus_tag="PLF85_00410"
     CDS             complement(18616..19431)
                     /locus_tag="PLF85_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HNE18141.1"
                     /translation="MSEVQAGALVGGGKNAAAPSVLQKINAESRGRAAQIQLPTRPPE
                     KSTIDFTAALRRRGAMPQLIAELKRRSPSKGSLKEGLPAADAVRIYEPYAAALSILTE
                     PNHFHGSLDDLREARSLTALPLLRKDFIVDEKQIDEARHAGASSFLLIVASLTDDELK
                     RFTARGRELGMEPLVEVLTEEELYRALALDIRILGINNRNLHTLEIDMNRCPSLAAKI
                     PAARREELVLVAESGYSTREELLALPHYFDAVLMGTGFMQASDPAAKIKEVFG"
     gene            complement(19428..20879)
                     /gene="mgtE"
                     /locus_tag="PLF85_00415"
     CDS             complement(19428..20879)
                     /gene="mgtE"
                     /locus_tag="PLF85_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNE18142.1"
                     /translation="MNTAEKKFHLLTKENYPKIVNLLRRLCKNRRFLLLKRALHTLHP
                     TSLALLWDSFDESEREIILTQLSSEYAAEFLTELGSDERTAIFRSKDTAWIFDRLEEL
                     ETDDIAGILRELDARDTNFILRRFDKNYTGKIKEILKYPEGSAGAMMTSDFLAVSRQA
                     TVKSIISQFQKLVKQNTAALKKAKGAAATHTKLDDLQFTFVVDRDNKFLGYIPLRKLI
                     LEKSTRKAHEIMQPAQVMVHPEQDRESVGKIFKDYNLLSLPVVDSRGILLGHITVDDV
                     VDVLEEEATEDILRLGGVTQDTQDAPHTHSIGRMARGRLPWLAINLVTQVLSAWIITF
                     FDQTISKVILLAPLMTVIGGQGGNATMQSVTVVVRSLALGQINSQNIWKVVLKEIGVC
                     SLNGLFIGTLAASMVLAYSQKPALAACMFIGLICNMFVAGAVGTLLPLGLRAVRVDPA
                     LASGPIATTFTDICGFSVFLGVATFLISQYGGL"
     gene            complement(20889..21260)
                     /locus_tag="PLF85_00420"
     CDS             complement(20889..21260)
                     /locus_tag="PLF85_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002126513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNE18143.1"
                     /translation="MHDSRAVRYTFITIGVISLLLGIVGIVTPILPTTPFILLSGFCF
                     ARGSERLHDWILSHRYFGPMIIAFRDEKRIPLKVKIFASLMIAVTMSITAFFIVPLLA
                     VKIGMGVVGVAVIGYIWTFRH"
     gene            complement(21322..21651)
                     /locus_tag="PLF85_00425"
     CDS             complement(21322..21651)
                     /locus_tag="PLF85_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE18144.1"
                     /translation="MLIVDKPFEKGVILSVKETRIDMSISRKFREQVAKFLEKKPAIV
                     VFDMHDTEYLDSSALGALVTILRDVKNYGGEIRLANLNQTLKTLFKLSKLESMFKIFE
                     TVEEAAR"
     gene            complement(21745..22686)
                     /locus_tag="PLF85_00430"
     CDS             complement(21745..22686)
                     /locus_tag="PLF85_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18145.1"
                     /translation="MPSQKPSLFARVRHRVNATSVTGALRGVFGDRLPKQWRGKMCFA
                     EDGRPLDGVPLVTRSHACIFVHGSADTELGWQPAPGELDYGRQLLLDFRVQPLYVRYN
                     SGLAIHENGAELSRLMLQLTQKNKSLRNLTLIGHSMGGLVIHSAVFDAQARGSAWAKK
                     VRQVFLLGTPHAGAPLAKLAEKTEQLLQFIPNPITLISASVIGIRSQGLKDLSKGQKG
                     ISTNDPVLLPHADYVFIAGGVSGKPGGLWNRLIGDGMVRHASALPASEKPSSDWKSLL
                     GRFARRRQVHIETLSGVGHLALRHSPAVYRILAAHWR"
     gene            complement(22687..23397)
                     /locus_tag="PLF85_00435"
     CDS             complement(22687..23397)
                     /locus_tag="PLF85_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE18146.1"
                     /translation="MKIESFGKAGSPRMIFLHGYGAHPHLYRDFIAAAAERYHVLVPE
                     LFGLSGVCRRDFAENMNVLRDKIAQHELDGTVVIGHSYGALAAMHFAAEFPQLRRAVA
                     INPLLPQLVNAGKLRVQLGNMQRDFAHATGEMRGMLANLAVGLRYGLNLLSNPVGYVE
                     GALRAVTTGLPRKSSAVPVDLVYADLDTLFHIEEADLGRWREVLPNLRLTEVPNYSHN
                     WIIYHGAFGWQKIAELLH"
     gene            complement(23448..24050)
                     /locus_tag="PLF85_00440"
     CDS             complement(23448..24050)
                     /locus_tag="PLF85_00440"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE18147.1"
                     /translation="MHPVLIIQGFLAPTVTNLVLKTRLKADGFLAEDVPLEGLNAGDI
                     RDSARIVEMSVNAMRTRAQVKKVDLIGISMGGLIGLHYLRKLGGDAYVRRFITIGTPF
                     HGTHLARWMRLLTLGRATGAEQMIPGSDFLKELHAHDAEHQAEIYSLHTSADAFVSEE
                     AAALKGARLVKSPHGVWPAGHYTPLFLPQDYSLIKEILQK"
     gene            complement(24108..24746)
                     /locus_tag="PLF85_00445"
     CDS             complement(24108..24746)
                     /locus_tag="PLF85_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE18148.1"
                     /translation="MTKIVNIVILEDRPVVQQRLTDLFSHECDFRITALCSAAEEVLP
                     RLEHIPCDLLIVDLELPGMNGEELIPLVRAKHPQTKMVVFTVFEDQARIVRLLRLGVN
                     GYLLKDTPDELLVAELKVILLGGAPLSPRVARKILDDERDQVWPDASPLSDRETEILN
                     LVALGMNYRAIADDLEISPNTVRVHIGHIYEKLAACSKVEALNRARQMGIIA"
     gene            complement(24743..>25048)
                     /locus_tag="PLF85_00450"
     CDS             complement(24743..>25048)
                     /locus_tag="PLF85_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18149.1"
                     /translation="EENPARVSDLALLHIDRIFSEWTANVIRHSGADKVAFGTRSRSG
                     HVCVWFYQNSGGFNWRGSATKGGLRSIALRAQNIGALVTVRRRGRGAYFILKMNTGI"
BASE COUNT         5759 a         7082 c         6851 g         5356 t
ORIGIN      
        1 gtacctccct gtagactatt ttacggaatc tcttgcctta cgtgcctttt gcagaagttc
       61 cttagaatac agctcacctg cgagtttatc ctgaacgagc agcgcggtgc gcttcagctg
      121 tgccagctct tctgcattgg gttctacctt ttcgattcct ttgccgagca aaacctgcat
      181 tgccttttcg ttttcgcggc ggagctcaag cgtcagttcg cgcgcatatt tttcgagcgt
      241 cgtatggacg atagtcttct gcgcgtctgt cagcttgtcg taagcagact tcgccaacaa
      301 ggtggctccc gctgcattcg ccaggcgcgc attggtgagg tactttgcct cgcggaacca
      361 ctgcaggcca atggccccat acgggggagc ataaaacgca tcaatgccac cagtcttgag
      421 ttgcggcaaa accgcttcga agccgagaaa cacaggaacc aggccgtatt cagaaagcat
      481 tgccaggacg aggcgatctc ccttgggagc ccagatcttt accccctgca tgtcagcgac
      541 cttcactatc ggcttatgct tgctcatcag gtaaacaaaa ccgttttcac cccagcctgc
      601 cagtaccagg ttctgcgcca ggaacttttt ggagagatcg ggatacactg cagcaacgac
      661 agcgtctagt tgacggaaat tatcgaagaa gaacggcagt tcaaacaaac gggattcagg
      721 tacgaccgta ccgagcgtct gcccggtaaa agatgcaata tcaatggtac ccgtcttcag
      781 cttgcgcatc accgagggct cgtcaccggc gataccacca taaaaaaccc gaacctttac
      841 ttcgccgtta gtcttcgcag aaatttcatc gacgcacttc ttgataactt ttgcccagat
      901 agtattctcg ggagcaaggg ttgcgtattt cagcgtaatc tcggcgctca gcggcgtcag
      961 cgtcaataac agcagcgtag caaaaaatac gcgcacgaaa gaactggtgg aaagcataac
     1021 ctaaccaact cgggctcaaa ctacggttgt acagtaattt tttttagcaa ctcttcggct
     1081 gccatttcga taagcggctc agaataaaag aatccctggg catattcgca gcccatttcg
     1141 gccaaaaggt ccctttcgtg gcgtgtttca atgccttctg ctacggcttc gagatgcagg
     1201 ttgcgcgtca gcgccagcat tgcgcgcaca atctcgtagt tgcggctacc atgcaccagc
     1261 ccgtgcacaa acgaacggtc aatcttgatg gtttcggcgg gtatcgaggc gaggtgactg
     1321 agggaagaaa accccgtacc gaagtcatcg atcaggatcc tgacctgcat ctgcctgagt
     1381 tcttcgagtt gcgacatacc gcctacgaca tctgccataa gcactgtttc tgtaatctcc
     1441 agccgtaaaa aacggggagg cagcctgaat ttttcgagca tacccgagag cgtgtcacac
     1501 aaatttccga aagcaaattg ttcgtccgaa atattgacgc tgatgtagaa gtccgcggca
     1561 taatggtggt gccagcgttt ggcagtctcg atggcgttct gcagcaccca ttcgccgagc
     1621 tgccgcatgg cctcggtttt ctcgagtatg ggcaggaata ccgcagggct cagtagcccc
     1681 agttcggggt gctgccagcg cacgagagcc tcaaagccgc tcagcttacc ggtggcgagc
     1741 gaatagattg gctggtaatg gttcctgagt tcgccgcgtg cgacaccctt ttggaaatcg
     1801 gaactcagct tcaggttgcg aaccgcgaca tcgtgcatct gcggctcaaa gagccgcacc
     1861 gagacccgac ggtcgctttt agcccgatac atcgcgttat cggcgtcgcg cagcatttct
     1921 tcggctgttc ggtaacgcag cgaagacatt acggcaccaa tactggcacc gataaagata
     1981 tctttgccct tgaaattgta gggagttcgg agtttttcgg caatgcgcat cgcgatacgc
     2041 tgcagcgcag cgccgtcgcc gacagggttc acgagtacaa taaactcgtc accgccccag
     2101 cgggcgacgc ggcagcgatc ttcaaccgag gcgagtaatt caaatccgac cgccttaata
     2161 agcagatcgc cgacatgatg gccgagactg tcattcacca gcttgaagcg atcgaggtcg
     2221 atgaagagga tgccaaagtt gcgctgcccg ctatgcaggt gaaagtctgc aatcgccgag
     2281 tggatggcgt tggtgatata atgccggtta tagagtcctg tgagtgagtc gtggtcggcc
     2341 aggtactgca gctcaaacgt cttggtagag acagcccggc gcaacatctt gatccaaaga
     2401 ccaattaccg ccaccagcac cacaatcgcc agcccgatcc ccacgaacac gttacgcatt
     2461 gcctgcaggt cactcatgcg gacgcggtta gactcaccga accatttctg gtagagtaca
     2521 tcgtacgtgc cgttggcctt aacgcgatca aggcccgaat tgagccattc aagcaggttc
     2581 gagttaccct tgcggacagc aaaggcaacg tttgaagcca gcaccggctg accggtggtg
     2641 atgacattgc gaataccgag tttctgcagg gcataacgcc cgccgacctg tgtcgtgatc
     2701 acgcagtcat gtttaccagt agcgagcagg cgcatggcat caaactggtc tttgacctgg
     2761 atcgatgtgt aacgaatacc cagcgtgcgc aggtactcag cagacaaagc tccctcttgc
     2821 tggataacct ctttgcccgc aagttcctgc accgacagta tcggctttga gccgatccgc
     2881 acgaaaattt ctgatgccag taccgagtga ggtttgctaa agtcggcgta gatgcgccgg
     2941 accggctggt cgaacatgct cacgacctgc acactgccgt cgagcagcga ttggcgaatc
     3001 ttgctccagg gccctgtaac aaaactgata cggcgcccct gcgcttcacc gattgccctg
     3061 atgagttcaa tctggaaacc ctcgagattt ccctcgtcgc tgaaccactc gtacggccga
     3121 aaggcctcgt cggcgccgta aacgattgcc ggctgttcgt cagcccaagc cgcgtcgaac
     3181 aggaagatca gcgataggaa cagcaggtgg ataaaacgca gcatcggtta tccctggtag
     3241 actgcaatgg cggtggtgta aagtgagatt agggcataaa aaacgggctt gcgcccgttt
     3301 tttatttggc tatcggccgg gaattagaac tggaagttta caccggcacg gataacatag
     3361 ccgttccagt cggctttggc tcctgatttt tccatttgac caccgcgata acccgcttcg
     3421 agcattaggt ttgttgctga cgcagaacct tcagtcagca tacccagatt aaacatggca
     3481 cccaacatac cttggtacat gaaaccgctg gcgctgtcgg taaaatcagg attcttctgg
     3541 ttatacatag aatatccaaa ccccaaacca gctgtaatgt aaggctgaac cgccggggta
     3601 tcttcgctgc ccatgggaat ataaagtcgc atgtttgcca tcggtataaa ggtataataa
     3661 gtttttgaaa ccgtcggata ctgattgata ccgttaacta ctacgggtgt acccgatggt
     3721 gaatcaaaac ccaatgcttg cacattcagc tctggcacga aggcaaaaaa tcgctcaagg
     3781 cgcatatcca acgcgatggc cgcgtctact gctgctttgt catttgctgc ccttacagat
     3841 gatacccctg cttttgcctc aaagcctaca gccatagtcg cagatggcag tgctgccatc
     3901 acgctcaaag cggcggcata aatgaacgtc gatttcttca ttttatctcc tttgtataca
     3961 ggaagctctc aggcttcctt aactgttatt agacgtatga cccgctactt gaggtcgata
     4021 aaaatgtaaa tccaaaaatg gtgtgtgtag ccatcacttt tgcagcgcaa aaagacgtac
     4081 cgccgcggcg atcgcctcga gcggccgtat gtctgccgcg cgggctgccc cgagtttagc
     4141 ggcagcgcct ggcatacccc agacactcga ggtagcctca tcctgaatca gtgtcggata
     4201 accactgtcg cgcagatctt ttaagccctg cgcaccatca ttgcccatgc cggtcagcaa
     4261 taccgcaagc gccgcccggg gcttcacttc aatcactgag ttgaataaca cgtctaccga
     4321 aggtttgtgc cggttcacag gtgcatcgtc accgaggcaa atgcggagct cgtgcgcttc
     4381 gcggcagacc cgcatatgcc gcccgcccgg ggcgatatag acctctcccg tgcgcagcac
     4441 catgccgtgc ccggcctcac agacggacac acgcgccacc tcgtccatgc ggcgggcaaa
     4501 agcgcgcgtg aaattttctg gcatgtgctg cgcgatcagc accggtaaag attcgctctc
     4561 gagcagccgc agaatgactt caagcgcctg tggccccccc gcggatgcgc cgataacgac
     4621 gagccggtct tcgttgcgct gcattgtgcc ttcgggaatc actgcggcag ctcctcgtgg
     4681 cgtacccagc agtcaccgtt ccagagttcg aaaatgacat ggcggtgacc ggttccgccc
     4741 acgtgctgcg ccgcaattgc cataccttca cgcgcgagaa ttgcacgcat catctgcagg
     4801 ttgcgttcac cgaccgtaat accggtctta tcgacgaact cgaacatttt gccgccgcca
     4861 aaaaccttca cgacgaaatc ctgcgggcgc gtgccggcac gcagcatctc gcggaaaaaa
     4921 atctcaagga cttcatcgac ataacgaccg ctgagttcgc tgtcttcaac gtagtctgct
     4981 tcatacccct tcggcaccag caggccacgt gatggcaaca ggcaatgcga catacctccc
     5041 actttgaggc gtgggtgcca gacacagatc gccacacacg agccgagcag ggtttttata
     5101 cgcacacggt tctcacccca aaaccagtcg cccggctgca ggtaaatttc gcgaccgcct
     5161 tcgggtaacg cgtgaaactc cgatctattg cggcgcatag attgtcggct tgaccgcctt
     5221 caggtgatca ctgatgccgt gcaggctttc agcatggccg atcaccagga acccgccagg
     5281 tttgaggctc tgcgcgagcg cctcggagac cttcttgcgt gtttctctgt cgaagtagat
     5341 catgacgttg cgcaggaaaa tcacatcgaa atagccaaaa ctgccgggcc tgtgctcaag
     5401 caggttcatg cgctgcaaac gaatgttttt cagaagcgcc ggggcgatac agaattttcc
     5461 ctgctcgctg cctgcgcctt tcaggcaata tttcttcagc aggtgctgcg gtatctcttc
     5521 ggccatttcg agcgggtacg tggcagccag cgcctgcgag agtatatgct cgctgatgtc
     5581 ggtaccaatc acctgccagc ccgtctcacc cagcacatcg gcgacggtca tggctatgga
     5641 gtagacttcc tggcctgaag agcacgcggc gctccagacg cgaaagggtt cgctgcggtc
     5701 gtgcgccgtc acacgctcgc gcagccagtc gaagtggcgt atctcgcgga aaaaataggt
     5761 ctcgttggtc gtgagcaggt tgatcatcat ctgccgctcg ccgcgatcat cttcactgcg
     5821 cacgatacgc aggtaatcgg tataactgtt caggccgtag tgccgcaggc gcttcataat
     5881 acgcgacgac accagggctt tttttgaatc gggcatgtgg ataccggccc agtcgtagat
     5941 cagcttctgg aagatcgcga attcggcatc ggtaagctct atactgcccg caacgggtgg
     6001 catgcggttc atggaatgtc ctctccggca gcgctgtagg cctgcggctc atagactgcc
     6061 agacgtatca gacgatttcc cctgaaacgg aaacggatgc cgcggccgta aacatcgact
     6121 tcattgccca ccacgcgcca cctgcgggaa cggtagaact cccccaggaa tctgccagcc
     6181 tgctcgctgt taagcccccg gagctgcgtt tcgtgcaacc ccagcgcggt aaaaaactct
     6241 agcgagggtg acttgtgttt ttcgatttcg aggtgtattt cgcgaatgcg gtcatttttc
     6301 atcacgaact ctgcgttgaa agttgcctcg gtgtaggtaa gcgtttccgg gtcggagtac
     6361 gtgcggtacg cgggttcacc gtagcgcgcg cgcaaatcgg tctccgtttc gccgagcagc
     6421 atacccgcag cgccggtacc ctcgatgaca tgcagcgggg cattgcgcag cagcagtatg
     6481 tctttttcgc tgggcagtgg cctgaaacga tcgtagctgg tacccgggcg ggtgccagcc
     6541 atagtgggaa atgccaaggc aacgagggcg aatatgccga tagtacagca tatgaccaaa
     6601 aagcgcgccc tgttcaaggc atacgaagat cctgaattca tgacttcttt tgatgcacgg
     6661 ccgcttcgca tacaggctga atacctgatg ccactgaccc attttgagaa attgcgcgtc
     6721 aaggatacca tcgttttttt cggctcagcg cgcctgccgg caccccacca ggccaaagag
     6781 gagcgcgaga agctacaggc tgccggtgaa tggaacgacg tcgaggctgc gcattttaac
     6841 ctgcgcctgc gctactacaa agatgccgtg cgcctggcgt acaagctcac acagtggtcg
     6901 aaatcgctgc ccgccgaagc gcgcaggttt ctcatctgct caggcggagg tcctggaatt
     6961 atggaagccg caaaccgcgg tgcagcacac gcgcgcggac tttctgtcgg catgaacatt
     7021 tcactgccgt ttgaacagga gccgaaccac tacatcacgc agacactcaa cttcgaattt
     7081 cattacttct ttatgcgcaa gttctggctg atgaacctgg cgaaggcgct ggtgattttc
     7141 cccggcggct acggtacgct cgacgaactc tttgaagtgc tcaccctggt acagacacgc
     7201 aaggtacaga agcgcctgcc gatcgtcatc ttcggtaagg aatattggga tcaggtcatc
     7261 aacttcgaag cgatggcccg gtggcgcatg atcaagtggg aagatatcaa gctcgtgcac
     7321 ttttctgacg agccgcagga tgcgtttgag tacctgacga agaatcttac tgagctttat
     7381 ctctgaatta tcggagaggc aaagcctctc cgataattca gagtctatcg agaatcgaat
     7441 aaaacatcgt atcgctgtcg aggtcgggtg agcccaccca gcgcgactca acgattttga
     7501 aaccaggatg ctgcgcgata aatgactgca cgacatcttc attctcagtt ttaaatacgc
     7561 tgcaggtact gtagaccagc cgaccgccgg ggcgcaggta accttgcact gcactgagta
     7621 tcttcagctg caaagcggca aacttcgcag gatcatcggg catgatacgg aagcgcacgt
     7681 cgggattgcg gcggagcgta cctgacgacg aacacggtgc atcgacgagt atacggtgga
     7741 aaccgccggc attcgtaacc tcgcgggttg gctcaacctg tgtctcggcg tcgtagacga
     7801 aacgccgtat gttatgaaaa cctgcgcggc gcgctctcag cttcaactcg tcgagtttat
     7861 agtcacgaat gtccgaggca tagagcacac cgcggttctg catctgcgac gcaatctgca
     7921 tagtcttgcc accaccgccg gcgcagacat cccatacggc catgcccggg tgtgcatcga
     7981 ctgcggcacc caggtgctgc gaagcgaggt cctggatctc gagccagcct ttcttcagcg
     8041 ctgccagttc gtaaacgggc agctccccct cgactctaaa tgtgacccaa cgctcgtcgt
     8101 gccgcagcgt ttcgacggaa aaacccgccg cgcgtagctc gacttcgatt tcgggcacgc
     8161 ggtcgatata gttggcgcgc aggtagagcg gtggtttggt tgcgagctgt tccagaaatt
     8221 gcggtatctt cgcctgtcgg attgaagaat gttcgagcca ttttttccag gtacgtggca
     8281 gagtgtctgc accagtagac tgggtgctgc gcgcccagtc taccgatgct aaggcgcgcc
     8341 aggcagaggc ggcgtcgcta gccgcttctt cgggcaaata accgatgacc gtgcgcagcc
     8401 ggcgaaagaa ctcttcgctg taccaacgcc ggtcgcgttt gccatagctc ttgtgctcgc
     8461 gaaaataacc cgcaagccac ttatcggcct gccgcgccat ggggtggtca atgtacgccc
     8521 gccacagcgc aaccaggtgg cgctcacggt tcgccccacc ccgcccttca ggctcccctc
     8581 cctttaaggg gaggggcctg gggggaggct ttattgcagc gcccctgaag ccactgccag
     8641 cagcgacttg ttgaaacggg caacgtcttt gatctcgccg ccgtcagcaa ttactgcgtg
     8701 gccccacaac agctgcgcgt agtccgcgag cagcggatcg cccgggttcg cttcgtagcg
     8761 cgcattgagc tttttcacca gatcatggtc gggattgatt tcgagcgtct tgaggcgcgg
     8821 cgctactttc tcgccgttgc gcgcgagaat tttctcgagg tacggcgaca tgtcgaagtc
     8881 ttcgccgacc acgcgcgacg gagcgctcac cagcacagag ctgaacttca cgtccttcac
     8941 ctggtctttg aggttctctt tcaggcaacc cagcagggca cccatggctt tttctgcctc
     9001 tttgactttc tcgctgtcct tttctttctg tttctcgtca gcgaggtcaa gctcaccctt
     9061 gctggctgac ttgaacttct tgccgttgaa ctcgccgatg ttctgcagca caatttcatc
     9121 gtacgcgtct acaaggtaga ggatctcgta acctttggcg cgcagcgatt cggtgtgcgg
     9181 ggagttctcg agcgtcaggc gcgactcgcc ggaaagatag tagatctctt tctgctctgc
     9241 cttcatgcga tcgatgtatt cctgcagcgt cgtggttttt tctttgtctg cagacgaggc
     9301 aaagagcaga agcccggcga gcttgtcctt gttctcaaaa tcggtcgaag cgccttcttt
     9361 gatcgccggc ccgaattcac ggtagaagtc tgcatacttt tcgcgctcct tctcgagcag
     9421 gtctgtcagc gttgaaatga tcttggtcgt cagacccttg cgaatcgccg tgatgtggcg
     9481 gtcttgttgc agaatctcac gcgagatgtt gagcggcagg tcagcgcttt caatgacgcc
     9541 gcgcacgaaa cgcaggtaac gcggcagcag ctcttcagcg cgttcaagaa tcagtacctt
     9601 ctttacgtag agctgcaggc cgcccttgaa gctcgtgtag tagagatcaa atggcgcccg
     9661 cttcggcagg taaagcaacg cgacgtattc gatacgccct tcggcgcggt agctgatcac
     9721 cttgagggga tcttcccagt cgtgcgagat gtgcttatag aactcgttgt attcttcgtc
     9781 tttgacgtcg ctcttgctgc gggtccagat cggcttcatc gaattgagcg tcgcttcttc
     9841 gatgctttct tcagttttgg cgtcctggcc ttcgccgacg gtcttgtaga tggtcttctt
     9901 catcaaaatc gggtatgtga tgaagtcaga atacttcttc acgaccgact caatttcgaa
     9961 ctcatcgcag aaatcggcca ggccgttttc tttgtcagcc ggcttgaggt cgagggttat
    10021 ggttgtaccc ggcatcgcac ggctgtcgtc ctcgatagtg tacgtgcctg cgccggtcga
    10081 tacccagcgg gtcgccgatt cttcgttggc acggcgggta atcatcgtga tcttgtcggc
    10141 caccataaag gcggagtaaa agcctacacc aaactggcca atcagatcga cggcatcttt
    10201 tgaagccttc gccttctcga tgagctcttt cgtgcccgaa cgcgcgatcg taccgatatt
    10261 ctgcacgacc tcgtcgcggc tcatgccgat gcccgtgtcg gtaatcgtga gccggcgcgc
    10321 ggtcttgtcg acttcgatgc gaatgaaatt gttggtttcc tgggttacag acttgtcagt
    10381 caaaccgagg aatttacgct tgtcaatcgc atcggacgca ttcgagatca gttcgcgcag
    10441 aaagatctct ttgtgcgaat agatggagtg gatcatgaga tccatcagct gtcttgtttc
    10501 agcctgaaat tcaaaggttt gtgttgtact catagtcgtt ttggaggtgg gccgcagtga
    10561 gcagcccgct cccaaactag tgtttgacat agggtgtcaa ttttgcagcg aaatgccgtc
    10621 accgtgcgtt ttggcctgca atttaagctc accctgctca tcgtcgccct gatcaccgcc
    10681 gttttcgtgg cgatgggaat ctacgtggca cgggagaaaa agaatattct taccgagact
    10741 gcctcggcca ccgcgaagaa ggagctcgcg agtctagcct tcatcgcctc gcgggcaatt
    10801 ctggaccgcg acgacctggc gctcgccgac tcgctgcaga atgcgcaggg cctgccgggg
    10861 tttacgattg gcgcagtcta cgtacccccg aaaaaaaatt cgctcatcaa ccgtacgggc
    10921 aaagagctcg gcgaaaagca gaaagaagtg gagcgggacg gtatagaaac agttcgtgcg
    10981 cactgggaca ccattgtcgc ctctcctgta gccttaggcc accgaataga aaaagcgttt
    11041 ctcggagaca aagacatcaa cgtccaatac tttcaccgcc cggtctttca ccccttcgtc
    11101 aaagttaacc ctcctctgct cggggtggca caggtcgcgg tcaccgacga gtacatcaac
    11161 aagtcgattc gcgcaagcct catcgagctg ctcatcgtgg gaggtatctt ttttatcgcg
    11221 gctctttttg cggcttacct gctttcccgc ttcatcgtta aaccggtaaa agtcctgtca
    11281 aaaggagcgg ccgaagtcgg caaaggcaac ctcgacgtca ccttgccccc gctaggcagc
    11341 gacgagctgg gacagctggc taaacagttc aacgagatga ccgcgagcct caagaaggcg
    11401 agtgaagagg caaaagaaca ggccgtcttc aacgaccagg tgcgacaggc aaaagaaatc
    11461 caggaaggga tgaacccttc gcgctttttg aaaaaacccg agtaccagat caagggcttc
    11521 acccgcgccg ccaagggcgt cggtggtgac tactacgact tccagatgct cggcgatggt
    11581 cgtatcgccg tgctgatttc ggacgtgagc ggaaaatcca tctcggcgtc gctcgtgatg
    11641 gttttgatca aaaccgttgt gtccacatac ctgaaacttt ttaataccat ccgcggcgac
    11701 acgatcatcc gcacgatcaa ccgcgtgatg tgcggcgaag cgcacatcga caagtttgcc
    11761 accatccagt tcttcatttt tgaccccgcc acaaaaacac tggagttttc gaacggtggc
    11821 catggccccc tgttcctcta ccgggccgac cgcaagatct gcaccgccag caaactagaa
    11881 ggcctgccga tgggtattga agaagagaac cagtacaagc tggcaaagat gcagctgaac
    11941 agcggcgaca tcgtgatgct ctataccgac ggtattaccg aatgctggaa taccgcaaaa
    12001 gaagaattca gcctgaagcg cgtgcgcgaa aagctgaccg aatacgcgaa cctcaaccca
    12061 aaagaaatca tcgagaactt cgtgcgtgac ctcgacgagt ttgcctcggg cgctgaacaa
    12121 cacgacgaca tgaccatggt ggtcatgaag gtgctctgag agccaaccca tgcgtgacga
    12181 aatcttcaag cgccccggtt cgggtaaaac acccttcgaa ttcaacgcgc aggtggccga
    12241 agttttcgac gacatggtct ctcgctcggt gcccttttac cgcgaggtgc tgcgcatgac
    12301 cgccgaactc gcgcatgact tctaccagcc gggcacgcag atctatgatc tcggctgttc
    12361 cacgggcgcg ctcgcgcacg cgctggctga agagttcggc aacgatactt tttcttacaa
    12421 cggtctggat aactcagaac cgatgattgc ccgcgcaaaa acgcacagcc cccgcgtgaa
    12481 tcccggttct acgaagatcg attttaaggt tgccgacata gccgctgtca actttgaacc
    12541 ggcttcggtt tttgtttcta actatacctt ccagttcctc aagccgctcg cgaggcaggc
    12601 actactacgc cgcatctaca ccgcgctcgc gagtgacggc tgcctgctct tgtcggaaaa
    12661 atgcctcgaa gactcggcag atgtctcgcg cctgtacgcc gaccactacc acgcgatgaa
    12721 agcgcgcaat ggttacagtg aactcgagat tgccgaaaaa cgcgaggctc tcgaaaacgt
    12781 gctgattccc tttcgggtga gcgagaacat cgagatgctg cgtgaagccg gcttcaaccc
    12841 ggtgtcgatc ttcttcaagt gttacggatt tacttctttc ctggcattga aggcgtaaat
    12901 ggcggggctt cagttttcgc tgcccggtta tttgcccgag aattcgggtg cgctgaccga
    12961 agaactggcg cggcaggaag aaaaactcgc aaccaccggg ctgaccgaac tgctggcacg
    13021 cattggagcg ctggcaggtt ccggaaaaac tcccgcaccg ggtttcagca tacaagatgg
    13081 cgctgtctgc agtgcccagc tgctcgcagg cgccgagcag attgcgcgcg atcttatccc
    13141 ttggcgtaaa ggtccgtttg aactcggcgg actgcagatt gacgccgaat ggcgttcgga
    13201 tataaaatgg aaccgcctga aatcccactt gcctgatatg gcaggcaaaa ccgtcgccga
    13261 tgtcggctgc aacaacggct actacctgta ccgcatcgct gcagcaggtg caaaaaatat
    13321 tctcggcctc gacccaacgc tgaagtactg gttgcagtac caactcatcg cagcgcatac
    13381 cgggcaagtc gcggactttc tgccactcgg ctggcaggtg ctgtggcaga tgccgcggac
    13441 tttcgacacc atatttctca tgggcgtgaa ttaccatgac cccgacccac tcgaaatgtt
    13501 tcatgcctgc agctacgccc tgcgcgaagg tggtgccctc atctgcgaat cggtggtagt
    13561 gcaggcggac gccgaccttg aaatttttcc cgaaggcaaa tacgccggca tcggtggcgt
    13621 gtacgcgata ccttcgccgc gtgcgctcaa gcggcagctc gggttcgtgg gatttcagaa
    13681 cgtcacgctg ctctactccg atccgctgct tccggccgaa cagcgccgca gtgaattttc
    13741 accacaggcg agcctcgcag atcacctgcg cgagggcgac gcgtggagca aagaaggtta
    13801 tcccccaccg tatcgcgcgg cgttgctgtg ccgagtctga ctttacgtgc catgatctgc
    13861 cgcgactgca tccgccgatg aaaaaaatac tcatggcact cgttgccgca accacactgc
    13921 tctcggtttc agtgagcgca aaaaccgaaa aacagattcg cgacgaaaac aaagcgaaag
    13981 cttacaaaga aaagaaagct gcagaaaaag ctgagcgaca ggcaaagcaa gagcagaaaa
    14041 aagccgcgaa gaagaagtaa gttgaacgga cgggttcctt ttcaggaacc cgtcaatccc
    14101 acacaaacaa ccatttgatt accacgtatg cgccgtagaa gacccagccc agcgggcccg
    14161 gttttgtctc tttggtaaac gctgaatacg gttcctggaa cgaaacgctg attgtcgaat
    14221 aaccaatctg gctgtcatag tagcgtatct ggcctttgag agtttcgacg cgctcggtga
    14281 tgcgcgagag ctcgcgttcg acctccaccg catctttgac gctggtgctt tttttcaata
    14341 gttcttcata ccgcgcaagc atcttgagct gcacgtcgag cctgatcttg aggtcagtgt
    14401 actgcatcgt cacatcctgc gccgttgcat tttcgtagcg aaccttcgcc aatgaacgga
    14461 taaagtcgtt catcgggtag aactccttcg agggcacgcg caccgttacc gaagtcgaac
    14521 cacgctgcga caggtaccca cccaaaccct ttgccttgtc gacgatgcgg ccactgagat
    14581 cgcgcgtttc agcctctttg gcctcgagcg taaagctggc gttataaatc agcagctggc
    14641 cgggcgtcgg ggcggtggat tgcgggcgtt ccggggcatc ggtcgaagcg gacacttctg
    14701 ccgcaggagc cggggctgcc ccagcgtcca tccgtgccga tttggcgccc gtactgcacc
    14761 caataatggc aagcagcagg aatggggtag atttcagaag gttacgcatg tggcctcttt
    14821 acgttaattt gaccgccggt ccatgcttct ggttcgaaaa aatgcggcct gccgtgcgac
    14881 cgaaggtctc tgcttcagag tttatcgaac ggcctgccgt gtgaccggag gtctctgctt
    14941 cagtgcttgt cgaacggcct gccgtgagct tgtcgaacgg ttgacggcct gtctcaggga
    15001 gacataaaga ccaacggcct aatctcccgt gcgtatcgcc gaacgcgggt aaccccaggg
    15061 atataaatgg atgcaccggc agtaaaaacc tgccgggcac agtaaactag gagcgaccat
    15121 ggcaacaatt tcaatgaaaa gcctgcttga agcaggtgta cacttcggac accagacacg
    15181 ccgctggaac ccgaagatga agaagtatat cttcacagcg cgtaacggca ttcacattct
    15241 gaacctgcag aaaacgatcg tactcgcgaa agacgcgtac gaagcgatgc gcgacctcgc
    15301 ctcaaagggc ggcaaagtac tctttgtcgg tactaaaaaa caagcacagg cagatatcga
    15361 aaaggcagca aagcgctgcg gccagtacta catcaacaac cgctggctcg gtggtcttct
    15421 gaccaacttc cgtactgtgc gccagtctat tcagcgcctg cgcaagcttg aagaagcctt
    15481 cgagaccaac acagtacacg aactcgtcaa aacaaagaaa gaaattctac aactcgaccg
    15541 cgaaatcgcg cgcctgcgca aagaccttgc gggtattcgc gatatgaacg atctgcccga
    15601 tgcactgttc atcgttgacc ctgagcgcga gtcgattgct gtaaaagaag cgaacaccct
    15661 tggaatcccc atcttcgcga tggttgacac caactgtaat cctgacctcg tgcagtatcc
    15721 tattcctgca aacgacgacg cgatccgcgc ggtagcgctc tttctcgaga tcatggcaaa
    15781 cgcgatcgaa gaaggtaaag aaggcggcga attcgcgcag gtagaaatcg aagaaggcga
    15841 gagcatggac gagcaccagc tgaaatcttc aatggaaaag ctcgatgacc tcgaagaaat
    15901 cgaatcaaaa tacgaagagg cgcgcaccta agcgggtcaa ctgacacgga gttacagata
    15961 aatggcaatt gaccaggaaa aaatcaagaa cctgcgcgag atgacttcgg caggtattct
    16021 cgactgcaaa aaagtactcg aagagaccaa cggcgacctt gaaaaggcag ccggcctgct
    16081 gcgtgaacgc ggtatcgtta aagccgtgaa gaaaatggat cgcgaagctt ctgaaggagc
    16141 tatcgtatct tacgtacacc acaacggcaa gatcggcgtg ctcgtgcagc tgaactgcga
    16201 gactgacttt gttgcgcgca acgagaagtt cctcgaactc ggccggagca tcgcgatgca
    16261 tattgctgca acgaacccac agttcgtgaa aggcgacgat gtatcgcccg atgttatcga
    16321 gaaagaaaaa gaagtccagc gtgctgctct catcgaagaa ggcaagccgg ctgacaaagt
    16381 ggataagatt ctcgaaggca agatcaagaa gtttgtttct gacatctgcc tgcttgacca
    16441 gccgttcgtt aaagagccgg acaagactgt cggcgacatc atcaaggcgt cgatcgcgca
    16501 gctcggtgaa aacatcgccg tcggccgttt cgtccgcttc gcgctgaaat aacttagcat
    16561 ggatacaccg gccgaaagac ctgcccacat tactgaggga agggcttttc gccgggtcgt
    16621 attgaagctc tcgggcgaag tactcgccgg agaagacaag cacggaatca acgtaaaaat
    16681 tatccggtcg ctggccgaag agattcgcga catccatcgc gaaggtgtcg agatggcgat
    16741 cgtcatcggc ggtggtaata tcttccgcgg tgtcaaggca gcgtcagacg gcatggaccg
    16801 cgcgatcggc gattacatgg gtatgatggc gacggtcatc aacgggctcg ccttacagga
    16861 ttttctcgaa aaagaaggat taaccacccg gttgcagact gcaatcgaaa tcaaatctgt
    16921 tgccgaacct tttattcggc gcaaggcgat tcgccacctt gagaagaaac ggattgttat
    16981 tctctcaggg ggcactggca acccctactt caccaccgac accacggcag cgctgcgcgc
    17041 ggtagaactc aacgccgatg tgatattcaa ggcgaccaag gttgatggcg tctacgacgc
    17101 agacccggcg aagaacccaa atgcgaaacg ctaccgccac ctcagcttcc aggatgcgct
    17161 tgtgaagcag cttaaggtta tggactcgac ggcattttcc ctttgtctcg acaacaagat
    17221 gcccattcag gtattcgaca tgaatacaga aggaaatctg aagaaagcta tccagggcaa
    17281 gccgatcggc acgctgatag acgctttttc ggagagcagg ttttatgagt gaagaagcca
    17341 acaaaattat tgcagacacc aaacaaagaa tgcagaagac ccttgaagat ctctcgcacc
    17401 acatggtgca gatccgctcg ggtcgcgcga cgccttcgct ggtagaagat atccgcgtag
    17461 aagtctacgg gcagaatatg cccctgaacc aggtcgcgtc tatcactgca cccgaaccgc
    17521 gcctgatcac ggtcgatgtc tgggataaat cgcagatgca ggctgttgag aaggctatcc
    17581 agaagtcagg caagaacctc aatccaaaca atgatggttc aatgatacgc atcagcctgg
    17641 ctgaaatgac gggcgaaacc cgcaaagaaa tggtaaaact cgcgaaggcg aaatgcgaag
    17701 accacaaagt cgccgtgagg aatattcgcc gtgacgcgaa tgacaacctg aaaaagctca
    17761 agggcagcag cgtttctgaa gacatgatca agggtttcag cgaccaggtg cagaagctga
    17821 ccgacgacta catcaagaag atggatgatc tttttgcctc gaaggaaaaa gacatcatga
    17881 ctgtttgaca gactggcctg accagtctgc caaacggaac aacatgccca aagcacccaa
    17941 acacatcgcc gtcatcatgg acggcaatgg gcgttgggca gagtcgcgcg gtctgaaacg
    18001 ggcggaaggc cacagggcag gtacagaaac tgtcgaacgc ctgctcgacc acctgattaa
    18061 agtcaaagtg ccatttgtca gcctctatgc cttctctacg gagaactggc gcaggccacg
    18121 gaccgagatc acggcgatat tcaaactact cgatgaattc atcatcgcca aattgcccaa
    18181 gatgattacg cagggtgtgc gcatcgtcac atcgggcgac atctcgcgtc tgcccaaacg
    18241 cagccgcgaa ctgctgcaag atgcagtaaa aaagaccgcg cgcgggcgcc gcctgaccgc
    18301 gaacttctgc ctcaactatg gctcgcgcgc agaaattctg cgggccgcag agctggcact
    18361 tgcagccagc aagaacggca aacttaccga gaagcaactt gcccgcgcgt tgtggcaacc
    18421 ttccataccc gatgtcgacc tgcttgtgcg tacggccggt gaactgcgcc tttccaactt
    18481 catgctttgg caggccgctt acgcggagct ctacttcacc gaaaagaact ggcccgattt
    18541 tgctgaaaac gacatcgacg cagcaatcgc cgaatttgga cgccgcaagc gcaagtttgg
    18601 tggcctgtag aagccttaac cgaaaacttc ttttattttt gccgcgggat cgctggcctg
    18661 cataaacccg gtgcccatca gcactgcatc aaaatagtgc ggcagggcca gtagctcttc
    18721 gcgcgtcgag taacccgact cagccaccag cacgagctct tcgcggcgcg cggcaggaat
    18781 ctttgctgcc aggctggggc accggttcat gtctatctcg agtgtgtgca ggttacggtt
    18841 gttgatgcct agaatgcgaa tatcgagcgc cagcgcccgg taaagctcct cttcggtcaa
    18901 tacttcaacg agcggctcca taccgagttc gcgccccctg gcggtgaatc gctttagttc
    18961 gtcatcagtc agcgatgcga caatgagcag aaaactgctc gcgccggcgt ggcgggcttc
    19021 atcgatctgt ttttcatcaa cgatgaaatc tttgcgcaag agcggcaggg cggtcaggct
    19081 gcgcgcttcg cgcaggtcat cgagcgagcc gtgaaaatgg ttcggttcag tcaggatgct
    19141 gagcgcagca gcgtaaggtt catagatccg caccgcatcg gctgccggca ggccttcttt
    19201 cagtgaaccc ttcgaagggc tacgccgctt gagttcggcg atcagctgcg gcatggcacc
    19261 gcgacggcgg agcgcagcgg taaaatcgat agtgctcttc tcaggtggtc tcgtcggcag
    19321 ttgaatttgt gcagcacggc ccctgctctc tgcattaatt ttctggagaa cgcttggcgc
    19381 agcggcattt ttgcccccac cgaccaatgc acctgcttgt acctcgctca taacccgccg
    19441 tactggctga taagaaaagt tgcgacaccc aggaaaacag aaaagccgca gatgtcggta
    19501 aaagtcgtcg cgatcgggcc cgacgccagc gccggatcga cacgcacggc gcgcaatccc
    19561 aggggaagca gcgtgcccac tgcacccgcg acgaacatgt tgcagataag accgatgaac
    19621 atgcaggcgg cgagcgccgg cttttgcgaa taggccagca ccatgctggc cgcgagcgta
    19681 ccgataaaaa gcccgttcag cgagcagacg ccgatttctt tcagcacgac tttccagata
    19741 ttctgcgaat tgatttgccc gagcgcgagc gatcgcacga caacggtaac cgactgcatg
    19801 gtggcattgc cgccttgccc gccgattacg gtcatcaggg gagcgagtag tataaccttc
    19861 gagatcgtct ggtcaaagaa ggtaatgatc cacgccgaga gaacctgcgt aaccaggttg
    19921 atagcgagcc agggcagtcg cccccgcgcc atgcgtccga tcgagtgcgt gtgcggagcg
    19981 tcctgtgtat cctgcgtaac gcccccgagg cgcaggatgt cttcggtcgc ctcttcttcg
    20041 agtacgtcga ccacgtcgtc gaccgtgata tgcccgagca aaattccccg cgaatcgacg
    20101 accggcaggc tcagcagatt atagtcttta aaaattttac cgacgctctc gcggtcctgc
    20161 tcagggtgca ccatcacctg tgccggctgc atgatctcgt gcgccttgcg cgttgatttt
    20221 tcgagtatca gcttccgcag cggaatataa ccgagaaact tattgtcgcg atcaaccacg
    20281 aacgtaaact gcaggtcgtc gagttttgta tgcgttgctg cagctccttt tgccttcttg
    20341 agtgctgccg tattctgctt caccagcttt tggaactggc tgatgataga tttcacagtc
    20401 gcctgccggc tcaccgccag gaagtctgaa gtcatcatcg cgccggccga accctcgggg
    20461 tacttcagaa tttctttgat cttgcccgtg tagttcttat caaaacggcg cagaataaag
    20521 ttcgtatcgc gcgcgtcgag ttcgcgcaag attcccgcga tgtcgtctgt ttcgagctct
    20581 tccaaacggt caaaaatcca ggcggtgtct ttggagcgaa aaattgccgt acgctcgtca
    20641 gagcccagct cggtcagaaa ctccgccgcg tactcgctcg aaagctgcgt caggataatc
    20701 tcgcgctcgc tctcgtcgaa tgaatcccac aataatgcca gcgaagttgg gtgcagtgtg
    20761 tgcagcgcgc gcttgagcag caggaaacgc cggttcttac aaaggcggcg tagcaggttg
    20821 acgattttcg gataattctc tttcgtcagc aggtgaaatt ttttctccgc cgtgttcatg
    20881 cagaattctc agtggcggaa tgtccagatg taaccgatca cagcgacgcc gacaacgccc
    20941 atgccgattt tgacggctag cagcggtacg atgaagaacg cagttatcga catggtcacc
    21001 gctatcatga gtgaagcgaa tattttcacc ttgagcggta tacgcttctc atcccgaaag
    21061 gcgatgatca tagggccgaa atagcggtgt gaaagaatcc agtcgtgcag gcgctctgaa
    21121 ccgcgcgcaa agcagaaacc gctgagcaga atgaacggcg ttgtgggcaa tatcggcgtc
    21181 acaataccga cgatgccgag cagcagcgaa ataacaccga tcgtgatgaa ggtataacga
    21241 accgcccgtg aatcatgcag agcgggcgca cggccggaaa caggcgcggt gccaaccgct
    21301 gggggtaccg gcccggcctt cttaccgggc tgcttcttcg acggtttcaa agatcttaaa
    21361 catcgattca agctttgaga gcttgaagag ggtcttcagg gtttggttga ggttggcgag
    21421 gcgaatttcg ccaccgtagt ttttgacgtc gcgcagaatc gtcacgagtg caccgagcgc
    21481 cgacgagtcg aggtactcgg tatcgtgcat atcgaagacg actatcgccg gctttttttc
    21541 gaggaacttg gcgacctgct cgcgaaactt gcgcgagatg gacatgtcga tgcgcgtctc
    21601 tttcaccgaa agaatgacac ccttttcaaa aggcttgtcg acaatgagca taccgtaaac
    21661 attgcgcgcc cgcggacgcg tcaattaaaa aaaccgctgt ggaatacgaa tggccacggg
    21721 tttgacctgg cgtcgacgct cgaatcagcg ccaatgggcc gcaagaatgc ggtaaacagc
    21781 gggcgaatgc cgcagcgcca ggtggccgac acctgaaagc gtttcaatat gaacctggcg
    21841 gcggcgcgca aagcggccga gcaggctttt ccagtcggag ctgggctttt cggacgccgg
    21901 caaagcgctg gcatggcgca ccataccatc gccgatgaga cggttccaga gaccgcctgg
    21961 cttgccagaa acaccgccag caatgaagac gtaatctgcg tgcggcagta gtaccgggtc
    22021 attggtcgaa atgcccttct gtcccttgct caggtctttc agaccctggc tgcggatgcc
    22081 gatcaccgaa gcgctgatca gcgtgatcgg gttgggaata aactggagca gctgctcggt
    22141 cttttcggcg agcttggcga gcggcgcgcc ggcatgcggc gtgccgagca gaaaaacctg
    22201 ccgtaccttt ttcgcccagg ctgaaccgcg cgcctgtgcg tcaaaaacag cagaatgaat
    22261 cacgagccca cccatgctgt gaccgatgag cgtcaggttg cggagcgatt tattcttctg
    22321 cgtcaattgc agcatcaacc gggataactc cgccccattt tcatgaattg ccagaccgct
    22381 gttgtagcgc acataaaggg gctgcacgcg aaaatcgagc agcagctgcc ggccatagtc
    22441 gagctcgccc ggcgcgggct gccaacccag ctcggtgtcg gcgctgccgt gcacgaagat
    22501 acaggcgtgc gagcgcgtga cgagcggcac accgtcgagc ggccggccgt cttcagcaaa
    22561 acacatcttg ccgcgccact gcttcggcaa gcggtcgcca aagacaccgc gcagcgcacc
    22621 cgtaacggac gttgcgttga cacgatgccg cacgcgcgcg aagagtgaag gcttctgcga
    22681 cggcatctag tgcagaagct ccgcgatttt ctgccagccg aatgccccgt ggtagatgat
    22741 ccagttgtgg ctgtagttcg gcacctcggt caggcgcagg ttcggcaaca cttcgcgcca
    22801 gcggcctaag tcggcttctt caatatgaaa gagtgtatcg aggtctgcat acacgaggtc
    22861 gacgggcacc gctgaactct tgcgggggag cccggtcgtt acggccctga gcgcgccttc
    22921 gacataaccc accgggttcg acagcaggtt gaggccgtag cgcaggccga ccgcgaggtt
    22981 agccagcatg ccgcgcattt caccggtcgc atgcgcaaag tcacgctgca tgttcccgag
    23041 ttgcacgcgc agcttgccgg cattaacgag ctgcggcagc agcgggttga tggcgactgc
    23101 gcgccgtaac tgcggaaact ccgccgcgaa atgcatcgcc gccagcgcac cgtacgaatg
    23161 cccgatgacc accgtgccat caagttcgtg ctgcgcaatc ttgtcgcgca gcacattcat
    23221 attttcggca aagtcgcgcc ggcagacccc cgagagcccg aagagctcgg gcacgagcac
    23281 gtggtaacgc tcggccgcgg cggcaatgaa gtcgcggtag aggtgcgggt gtgcgccgta
    23341 accgtgcaga aagatcatac gcgggctgcc cgccttacca aaactttcga ttttcataga
    23401 cagagcagca cactacaggt gcgccatggt acggggcaag aacaatacta tttctgaaga
    23461 atttctttta tcagtgaata gtcttgcggc aaaaagagcg gggtatagtg ccccgcgggc
    23521 cagaccccgt gtggtgattt cacgagcctg gcgcccttta gggcagccgc ttcttcgctg
    23581 acaaacgcat cggccgaggt atgcaggctg taaatttctg cctggtgctc ggcgtcgtgc
    23641 gcgtgcagct ccttgagaaa atcagaaccg gggatcatct gttcagcgcc ggtggcgcgg
    23701 ccgagagtca gaaggcgcat ccagcgtgca aggtgtgtgc catgaaaagg agtaccaatc
    23761 gtgatgaagc ggcgcacgta cgcatcacca cccagcttgc gcaggtaatg cagaccgatg
    23821 agcccaccca tgctgatgcc gatgaggtcg accttcttta cctgcgcgcg cgtgcgcatt
    23881 gcgttcacgc tcatttcgac aatgcgtgcc gagtcgcgta tgtcacctgc gttgaggcct
    23941 tcgagcggca catcctcggc gagaaagcca tcggccttaa ggcgggtttt gagcacgagg
    24001 ttcgtcacgg tgggtgcaag aaagccctgg atgatgagga cgggatgcat ggccttcagt
    24061 cagcaatttg tcggcgttga caatgaaaaa cggttatcgc gacgagctca cgcaattatg
    24121 cccatttgcc gcgcccggtt cagggcctca actttactgc aagccgcaag cttctcatag
    24181 atatgcccga tgtgaaccct gacagtattc ggactgattt ccaggtcgtc agcaattgcg
    24241 cggtaattca taccgagggc aaccaggttg agaatttctg tctcgcgatc agaaagtgga
    24301 ctcgcatcgg gccagacctg gtcgcgctcg tcgtcgagta tcttgcgggc aacccgtgga
    24361 ctcaggggag cgccaccgag caatatcacc ttgagctctg caaccagcag ctcgtcgggc
    24421 gtatccttga gaaggtaacc attcaccccg agccgcagca accgtacaat gcgggcttgg
    24481 tcttcaaaga cagtaaacac gaccattttg gtttggggat gttttgcgcg gaccagcgga
    24541 atgagttctt cgccattcat gcccggcaat tccaggtcga cgatcagcag gtcacaggga
    24601 atgtgttcga gccgcggcaa aacctcttcg gcagcgctgc agagtgccgt gatgcgaaaa
    24661 tcgcactcgt gcgaaaagag atcggtgagt cgctgttgaa ccacaggccg gtcttccaat
    24721 atgacaatgt ttacaatctt cgtcatatgc ctgtgttcat tttcagaata aaatacgcgc
    24781 ctctgcctcg cctacgtacc gtaaccagcg caccgatgtt ctgcgcccgc agggcaatac
    24841 tgcgcaaccc acccttggtt gctgaaccac gccagttaaa tccgcccgaa ttctggtaga
    24901 accagacgca tacgtgtccg ctgcgagacc gtgtaccaaa cgcgaccttg tcagcccccg
    24961 aatgcctgat aacgttcgcc gtccattctg aaaatatacg gtcgatgtgc agcagcgcca
    25021 aatcagaaac gcgtgcagga ttttcttc
//
LOCUS       DAOQJF010000004        24937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_883_length_24937_cov_5.533398, whole genome shotgun sequence.
ACCESSION   DAOQJF010000004 DAOQJF010000000
VERSION     DAOQJF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 24937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24937
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_883_length_24937_cov_5.533398"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLF85_00455"
     CDS             complement(<1..709)
                     /locus_tag="PLF85_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18150.1"
                     /translation="MRHNYIRIQTVADIDTSRITLRSIENRYIDSQGRRYATRFNLRT
                     RRIEIVPIALGREEAALARRLFSITSETNAEQAPESRGQRRVHPSPLKLPAWLAAMDV
                     EPDMSESELHGFLVTAEREMQRNGERYRGLIQSLKMSSILEEVKDSNQHDAILELTNT
                     YERDIQAHAARVQAMVVEFLRFPKPLASYLGQVDRNSKKYVEKLEMDKQKRYCMAYLL
                     AGEMLGVFKGCMELIEMV"
     gene            complement(709..>2636)
                     /locus_tag="PLF85_00460"
     CDS             complement(709..>2636)
                     /locus_tag="PLF85_00460"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE18151.1"
                     /translation="EFVTHVHGELAREMFWNVRLAIVALMGAMLLYYVMLMIDSGIQH
                     NLFWFIIQFSFGVPIMVFYFFGENWIKSVPRRVQFANLQAGIIAAVIGIGFVFQMPEI
                     KNQSIKLTAFSLIVGVTGAAAFTFACNRFTFLFFSVPWLLPIQLYFFFGAADLGSIIL
                     GGMVVPYVGVLTFLCLKDFERRVELINAELTVLEEKEQVVTASKKLKTSLDLVNELKT
                     QQDGDYYLTSLLLNPLGVNAVDEGLNVDVDFFTKQKKNFEFKNQKKTIGGDISIAHTV
                     QFSSGPATVFLNADAMGKSMQGAGGSLVLGAVFQSIIERTRLQASIDPQTWLKNTFLE
                     LQKVFESFDCSMLVSVYIAALENNSGMMYYLNAEHPWGVLYREGKAIFLQNRSYYRKL
                     GTPGLQDDIAIDQIQLKPGDIVLLGSDGRDDVALPSTEAGVRVINEDESLFLKHVETA
                     DARLSEIYDLIAATGEITDDFSLVRVQYNAQREAGSVKEKQTASVLLRDAKHLVDSGR
                     RDEAASLLRRAYHSSSAGPRALRAALNLAMQIKEYEIASNIAEKLLKVQPDDQEVMYL
                     AAFAERKLNNLEKALSYAGKVRNGESSPNVMNLNLLAEIYHAMGQRERAISVLDEILT
                     IDPLNAKARRLREEIHA"
     assembly_gap    2637..2736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3001..3294)
                     /locus_tag="PLF85_00465"
     CDS             complement(3001..3294)
                     /locus_tag="PLF85_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18152.1"
                     /translation="MDWSQIMIRSLQEGSIRELHLYQMPVLKNVENWTQVKEIGKVDF
                     RGKHSDYKGAIVQYGSNYFFLPNARIDALSVYRKWNFKKNIKVITEADHKKKG"
     gene            complement(3367..4533)
                     /locus_tag="PLF85_00470"
     CDS             complement(3367..4533)
                     /locus_tag="PLF85_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18153.1"
                     /translation="MRRALLFLLVVMPVRSDRIEFQGGVLRNAIAPTADYGALVYRHE
                     GFRIGTFTAPAAQGVVASYSSAQGPLLVEVGQRERSLESGFLVGNGAWLPWTRNFAFA
                     EPTGIRTGYELRYAGADLQYYQLDQNNLAAARIYLTPLDWLRLTGGVAVPEMHEGAQQ
                     LPIASLALGRAADRTGFSTGFEMVGEGNYLLHARYRGDFSARGLFFRRQQASPLASGI
                     FEQREGLALQFFERNWFAQFFSTDSRFGMLRYGGDLLSGVVVYEERMQLAGLSVRSDA
                     RGLHVRAGATVGHDTSIQSLFGLGFADAVFVGGGHYELFSEQPLEPIIFPADWYSSVL
                     LQSTAMRLRNSGFKLMALVDTEAVQGFVAVTYSEDVRGRERFDFYLRLAGGVTF"
     gene            complement(4526..5446)
                     /locus_tag="PLF85_00475"
     CDS             complement(4526..5446)
                     /locus_tag="PLF85_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18154.1"
                     /translation="MAKACLFAATLLLPQIVYSLSARLQRDTGNAAARSPVTGDQIEA
                     AGSYGSISYNLYARRDDFAGDRSLLNAAALVYNGFAVEDHRFAFGLGSHYRQEKLQRI
                     RAGQGDFYPAVGFFWSHPQFRADISGSQYLTRVSLSGLVDLGLPVELNSDFEYLHSQP
                     YRWSANAFFFISRYGGAIAGFEPLSQRVRAGLWLAPVKALRISALSRFGAGETFWELA
                     LSFQFDTATQAQGELPRYHANAEPPEEDDEPKKWQRRKPKNVPAFAVLVKWGLSPVEA
                     LKFTREKDACTLSEKAQRALAARNWGCRDA"
     gene            5714..5938
                     /locus_tag="PLF85_00480"
     CDS             5714..5938
                     /locus_tag="PLF85_00480"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HNE18155.1"
                     /translation="MVKRLRLEVPAKRVKEPFLYRMVTEYNLQPNILEANLSPDRCGS
                     MLLELKGKPQDIEKGILFLREADIEVAADD"
     gene            complement(5939..6700)
                     /locus_tag="PLF85_00485"
     CDS             complement(5939..6700)
                     /locus_tag="PLF85_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18156.1"
                     /translation="MKNLPQKKHEQPRKSAAVRGGSGKQFFTSPEPKVKAAQSAPDFR
                     VRLGGVPLEQRAGFELLSLRLRQLLRNTPETWETIDREEAGSFFSLFRKYHLPLLLPL
                     AFFFVVQEAIHFVNIRLFLRHVAVVFPALLGLYAGYIFLIGVIAEEVAEHSGGRFVPQ
                     SGMRIALFSTLILSFLSVAAFLPVVGKPLLALGIFWHYRQMFTGARALLNISDSNYRL
                     FQLGHLLVWVLMGLAGFLVLSVISFLFAKLGVAAI"
     gene            complement(6697..7998)
                     /locus_tag="PLF85_00490"
     CDS             complement(6697..7998)
                     /locus_tag="PLF85_00490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE18157.1"
                     /translation="MYSRFKAYLFLTGLVFTVIGVLAWFQSRTAPAEPTPGPAPAHGA
                     SASEDVNRLISVTEERPADTAAAASAVIPPVVASEIPAQWREDLGVPVLCYHQIVTEA
                     QYRERPTPYAVTVAQFKAQMQWLADQNFYTVLPDELLAYVKGEKQLDFSNGRRPIMIT
                     FDDGNNDFINHAQKVLEAHKFKGVLYIYPTYIMARKERSMTWAEIQAARKAGHAIESH
                     TMWHPMLSTMTDAEQRAQFVDSKKMLNEKGGANVRHLAYPFGIYNASSIKLLREAGYV
                     SAGTTFHGANQVGEHPYLLRRYLIVKGDNEKIFAQKTLARSLPLRLLNSEPGAVISGE
                     TELALRVPAGLDRNGFRVRVFSTPQTFNYDVKTGNLNVTVKPSKKRLSVLEIVYKEKG
                     IEYRANALLNHRRTAAGDGEEPKPAKKKKRLKKKKRNADDE"
     gene            8087..9946
                     /gene="ilvD"
                     /locus_tag="PLF85_00495"
     CDS             8087..9946
                     /gene="ilvD"
                     /locus_tag="PLF85_00495"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNE18158.1"
                     /translation="MPQLRSKTSTHGRNMAGARALWRATGMKEADFGKPIIAIANSFT
                     QFVPGHVHLKDLGQLVAREVEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHTADALICISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGKVNWEGSVR
                     KLDLVDAMVEAANDKVSDAEVQAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSTLATHADRKQLFLKAGRLAVELAKRYYEQDDASVLPRNIANFQAFENAMSLDVAMG
                     GSTNTVLHILAAAKEGGINFTLRDIDRISRRVPCVCKVAPATQQYHMEDVHRAGGVMA
                     ILGELDKAGLINRDVPTVHSPSLAAALDQWDVTRPTASEEAKALFTAAPGGVPTQEAF
                     SQSRRWETPDLDREKGCIRSVSHAYSKDGGLAVLFGNLAPEGCIVKTAGVDESIWKFS
                     GKAIVMESQEEAVSRILANEVTAGDVVVVRYEGPKGGPGMQEMLYPTSYIKSKGLGKS
                     CALLTDGRFSGGTSGLSIGHVSPEAADRGLIGLVEDGDVIEIDIPNRRIELKVADSAI
                     AERRKAMEARGKDAWKPKSRQRPVSASLRAYAAMTTSAHTGAVRDVSQVERQG"
     gene            10032..10295
                     /locus_tag="PLF85_00500"
     CDS             10032..10295
                     /locus_tag="PLF85_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18159.1"
                     /translation="MIHDIKTSKLALSGLVWLLVAVTWGATVTMLFRRTVPGDKVANL
                     TTNPNGLRVWLPDNSETAAGNTNSFVHSQNKAVVNRGEGKNGI"
     gene            complement(10394..11752)
                     /locus_tag="PLF85_00505"
     CDS             complement(10394..11752)
                     /locus_tag="PLF85_00505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE18160.1"
                     /translation="MKDLRNKVVLITGAAGGIGLNTAGFFAEAGSRLVLTDINELALE
                     NAKQRLAKHGVDILTKVVDVTDLKAVEALRDDVQQKFGGVDILINNAGIGFNAELVDT
                     PLSKWQQLMNINFWAPLYHIQAFIPQMKARKSGHIVNVASGQAYFRAPTWGAYSVTKL
                     AVGAMSEILNVEVARDNIKVTTVYPFLVNTGFYDTTSAESIGEKLFFAFMPLYSDKPE
                     TVARMIFDSVREEKDVEMVSILNQFFKYIRVLPSAAGIMDKTTHTFMSKQQQESLGIG
                     GVVDQVAGIVNSILDTAKKGVPEKGFQIHEVMSGEHEFVDGFGPAGKHKFEFKADWGP
                     KNLVDFMNPAADKFMLSELAGTVTIGGMCENMPVYGTLQLRYLQDQKIRYTFDFTVDG
                     EPYQYVGEKQQIYPWNLPYSHTTCFGEVKNLRTGKVISKSVTHFDMHDLPEFLGTFRL
                     TG"
     gene            complement(11816..12529)
                     /locus_tag="PLF85_00510"
     CDS             complement(11816..12529)
                     /locus_tag="PLF85_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18161.1"
                     /translation="MKSVKPLLLFLAGCSLLCQLMPASGLWADKARPDIVELINLLKP
                     GSLLRQSGGWVDTWVGESMDPAVVDATNGYAAFLDSGTGGGKILHEFAVYYPDGAGAI
                     LVHNFQDDRDTYVSEIKFYQLQATKLQPIEPPLVKPVCRDLASAATIKGFYKNPMLSG
                     LDPDPILPTFELPRKGTTILAYCSGNLNRFQVQQTMAGKLDNNTDLQEKIFQSIHFTS
                     KVQYLWNKKKGAFTAKKTR"
     gene            complement(12579..13388)
                     /locus_tag="PLF85_00515"
     CDS             complement(12579..13388)
                     /locus_tag="PLF85_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNE18162.1"
                     /translation="MLKPRVPEKLRELVAKFQINEAHYKSAEFLETQVRVEFINPLLR
                     LLGWDVDNEAGYAQDYKDVIHELHIGNKRHVDYAFKVGDSYKFIIEAKRPAADIADDA
                     YPVYQARKYAHDTKIRLVIVTDFEEFAIYEGYSKYDRAPEPTDGVEYRRVFYCLYTEY
                     ARYWSFLYSLISRKSVMLGLHDHLLARLEEVKNSIGNSVYTVREFNLLYSIAQSFGGE
                     DYTVEQDIEIDQPLSESLPVEAAKTAIAPAPKSMRQQPIEQPAFYLGPVFS"
     gene            13470..14192
                     /locus_tag="PLF85_00520"
     CDS             13470..14192
                     /locus_tag="PLF85_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18163.1"
                     /translation="MDLNCRRCHKPIAVENINIQQMIAKCGGCGAVFTFGPADASRET
                     HSPGGTKATVPRPGSWQVAREGNQLVLTRKWFKPLFLFLLLFSFVWNSFLVGWYSLLG
                     GIGGEAGFSAFSLIFFIFPLGHVAVGLGLAYYTLAGLLNRTIITVDRSALSIKHRPLP
                     WRHSGMIAAHELDQLYCKEERQSNKGSTWYTYTVSALLRDGRERKIMSGLDSPEEALF
                     LESEVEKYLGITDRPVAGEYRV"
     gene            complement(14202..14861)
                     /locus_tag="PLF85_00525"
     CDS             complement(14202..14861)
                     /locus_tag="PLF85_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE18164.1"
                     /translation="MKTTGLYLLLAFQALSAVEGRITYLSGQAEVRSKGKSQPLKLGA
                     TINEGDVIVTSARTKVGVQLSDGASFFMNPFSTLEIRPDSNYYQATGAVSMLFRKEGR
                     DNSNRWTIKTPVVTAGVRGTGFTVEARKSSVRIVLFTGKLIMTDFVRETGLQTDPNLM
                     MQDFLNDIELNAGAALTYDGAEVKKSRVDLAKEPLKQLHDEHQQLEKNPEWRKGAQDL
                     R"
     gene            complement(14871..16196)
                     /locus_tag="PLF85_00530"
     CDS             complement(14871..16196)
                     /locus_tag="PLF85_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18165.1"
                     /translation="MPPNTGTDVTVCAPGSSDVCYLSDLNGVNQPLDTQTRGLAVPNR
                     LIESDMNMFMNPGQLMNYGTAYLEGWLGANLVWGGATVPLPANQKLAVFVRRPLNANS
                     ALGSTQALFDKYAQTGHLTNGLTGFDGSFTGADLAILNKTTKGFGNLDLMYGMALGDL
                     NLGLRVSYANMRNNVDQSVAATGVNGKYTMSAHNIGVGVGAQWKNLGPGYLDLSLSSD
                     LPLMNMEYNATTTTGAENLTVRSSAAPSIGLLGRYVTPVGQDRLLVAANIDQYKMPYE
                     IRGTNLAGSVKSQDAAASALNLQLDAAYHQNFQDGKLKVIYSTGIGHTSVKYTVVDNN
                     NPAALNNSYDKNHLFVPVGVAVEHATFETLKTRFGVRKNVFSSRTTTDKTTAQTIETS
                     RAFYQDDELTVAMGLGWVPAEKVNLDFAMNANAFKLDTFFAALSARYHY"
     gene            complement(16303..17160)
                     /locus_tag="PLF85_00535"
     CDS             complement(16303..17160)
                     /locus_tag="PLF85_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE18166.1"
                     /translation="MSHLIQGKTKDLGDGFNVLRILPHVDARSVGPFVFVDHFGPAPV
                     VTGDELTVRPHPHIGLSTITYLYDGAVLHRDSLGTELVIRPGEVNWMTAGRGIVHSEH
                     STKENLPAKIEGIQTWVALPVEHEDTAPTFEHYRSDVIPVIEGEQWKLRLIAGTLMGK
                     VSPVKTHSPLFYADLETTGAAIIELELPPGEEAALYVSRGSFTAFGHTAQVGAMIVLT
                     GETRVQLSSHSAARAVLLGGEKFAEPRHLWWNFVATSKERIERAKAEWQANTMGIVAG
                     ETDRIPLPQ"
     gene            17253..19784
                     /locus_tag="PLF85_00540"
     CDS             17253..19784
                     /locus_tag="PLF85_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE18167.1"
                     /translation="MQPTQFEDTPLKESLKKFSGICLALAFLISLSGLCGWSFDIRWL
                     RRPLPQFVAINPLTAVLLLLAAVAQLLAIRRTRNNSAIVASIGMIITGTALAKLVDLF
                     SPGIAPVDQLLFAQAVDQERIANLPNRMAPNTAFCFLWLGFATILQPLGQKGKFVPAQ
                     FALIPLVLAALLSILGYLYRVPKFYGIMNYIPMALHTAAGFLSLAIAHITATPEQGIL
                     RHFTTNLAGSRLARWLVPATIFAPILLGFLRLLIGWQYGVPVEFGVAILVLSIITILT
                     LFVWFTTRALNLKDYEQNMVRAELAQVNAHLEKTVNERTAAALKTETRLRQVLDAMSE
                     GFQIIGFDWRYLYVNDAVARQAKVPKEVLLRSTFLELFPTSVNAELMEAFRRCFKNRE
                     ALHMENRFVFPDGSDGWFDLHFQPVPEGLFILSSDITHRKLAEIEILKLNEELEEKIR
                     ARTAQLQEVNANLEHLAQERATAVLQTESRLQQVLDNMLEGIQIIDREWRYVYVNESL
                     LKQAEKAREELVGFRVMDLYPGVEQSELFATLERSMNERRAQQIETAFRHSDERSGIY
                     EMSIQPIPEGIFILTVDITERRRAEEQLREANERLESKVRDRTRQLKELNQELESFSY
                     SVSHDLRAPLRTLTAYTQLLQRLSGTNLSEECKDILVVIEKKVEQMNQLISDLLAFSQ
                     LGSQKIHPAPIDMDLLVADVLRELFPEGIPANLVLNIDKLEAAYGDANLIQQVIVNLV
                     SNAVKYSARQEQPTLKIHSSVVEGEVIFEIRDNGVGFDMTYADRLFGVFQRLHSSTDF
                     EGTGVGLAIVQRIIHKHGGRVWAEAAPGKGARFYFTLPSVKVAKS"
     gene            19871..20227
                     /locus_tag="PLF85_00545"
     CDS             19871..20227
                     /locus_tag="PLF85_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18168.1"
                     /translation="MHPAFAQEKPLPVLQYVDGNGNSYEFTEEGSGYLLEFEPVKKGK
                     YSGGEPASRRVSKENYERLLARMDETIKKKRLTSTRERGTALIIKLVKGDVKEYILPK
                     GSKDIKEIEALLYTLF"
     gene            20245..20964
                     /locus_tag="PLF85_00550"
     CDS             20245..20964
                     /locus_tag="PLF85_00550"
                     /inference="COORDINATES: protein motif:HMM:NF021371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HNE18169.1"
                     /translation="MASPFLTARWKNLFMANYEVDAGLLQPLVPTGTELDLYQGRALV
                     SLVAFQFLNTRVKGFRIPGHVNFPEINLRYYLKRTVEGETRRGVGFVREIVPRRAITL
                     VANTLFNENYATRKMGQRVRVSGDSLKAEYTFVEKGREQKISCVTGITPQALKPGSLE
                     EFITEHYYGYTKGRRTLEYRVEHPQWRTLQVQDYAVGVDFGLAYGEQWSKLTGLRPHS
                     VLFAEGSEVNVFSAGKIIYGS"
     gene            20961..21203
                     /locus_tag="PLF85_00555"
     CDS             20961..21203
                     /locus_tag="PLF85_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18170.1"
                     /translation="MRMGQAEIMDVHDAVVLLKAQDLSVEIAALDELTADVQKKLAGS
                     EMPQWHKDILDDRLSGDEKFSPWEVVKARLQNGAKG"
     gene            21187..21504
                     /locus_tag="PLF85_00560"
     CDS             21187..21504
                     /locus_tag="PLF85_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE18171.1"
                     /translation="MALKVEVSASCEIDLEQAYQWYESHRAQLGLEFLNDFDERLNLI
                     AVNPYIGAEVYRQTRKFVMTRFPYIIYYLLTDDHVKVVACLHGGLDPRLSAGTVHNRI
                     IEQ"
     assembly_gap    21545..21644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21645..23370
                     /gene="ligA"
                     /locus_tag="PLF85_00565"
     CDS             <21645..23370
                     /gene="ligA"
                     /locus_tag="PLF85_00565"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNE18172.1"
                     /translation="AFHDRLVKAGLAEPIYHIEPKFDGLAIDLVYENGVLTVGSTRGD
                     GETGEDITHNVRTVANIPLRLALDKPPKRLSIRGEVIIKLADFHKLNEQQAKDGKKLF
                     ANPRNTAAGALRQQDSSEARNKKLSFFPYTLAHSPEVQKYFGKDMRTQEAIWNKVFPA
                     LGFKISAYHRTADIEGIIAYYEEMTAKRADVEYDLDGLVVKLNDTTEWENFGATTKAP
                     RWAIALKFPARSGITKLERVVYQVGRTGVITPVAELTPINLGGVMVSRASLHNADEIE
                     RLDLHEGDYVEVVRSGDVIPKVVGAKPEQRAKKAKRIAFIKKCPSCGKALEREDVFFR
                     CVNAACPEMNEAYLQFFVSKNGLDIESLGSEWVAKLYQNKVIADYADLFAVTKEDLLQ
                     FEGMGEILATKILQSIDTRRQVPFTKLLTAVGIPNVGEHVASILAEHFEGPDALAQAS
                     LEELTQIHEIGPGTAASVQAWFADKVNTSRLKKLQKNGVEILRAKRQRVSDVFAGKTF
                     VFTGALSQFTRESAEEAVKARGGRAASSVSKKTDFVVVGADAGSKAQKAKELGVTILS
                     EEEFAQML"
     gene            23427..>24937
                     /locus_tag="PLF85_00570"
     CDS             23427..>24937
                     /locus_tag="PLF85_00570"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNE18173.1"
                     /translation="MKAVVSDFTRAIWESLGWEAFGVDDRIEIKDVTQDSRQVKKGSA
                     FFAYDGHKVRGKDFIWDAFDRNAAAVFVDREYKEEIFNDSRYNKNLPLFFNHDFQRGA
                     GRTISFLYGEPTRYMSCIGVTGTNGKTTIAHSLYQALSHLNKPSMYIGTLGMEVSRHK
                     SQTGMTTPDTVNLQHMLHQGQKQKALYACLETSSHGLAQGRLEGINFQIGIFTNLTQD
                     HLDYHRNMEAYYRAKRRLFEYMLFASRKQPETAKGSIVCIDDDYGRRLYHWLKGQNPS
                     FPVISLSTRDKNADAYITDVEATFDGYRCVVVFAQKSYFIKTRLLGRFNLVNLASVFL
                     SLLCLGFGTDEALEAIQQVEPVPGRFEVLRGLEQRTVIIDYAHSPDALAKVLETAQEL
                     TPARVIGLFGCGGDRDKEKRPQMAEVASLHADLCILTNDNPRSEDPKNIIEDMRKGLK
                     TARYLVITDREKAIRAGLQILGPRQVLVIAGKGHEDYQIIGNKTLHFSDRETAL"
BASE COUNT         5712 a         6844 c         6736 g         5445 t
ORIGIN      
        1 cgaccatctc aataagttcc atgcagccct tgaaaacgcc gagcatctcg cctgccagca
       61 ggtaagccat acaatagcgc ttttgtttat ccatctcgag cttctcgaca tacttcttgc
      121 tattacggtc tacctggccg aggtaactgg caagcggctt cggaaaacgc agaaactcca
      181 ccaccattgc ctggacgcgc gcggcgtgcg cctgtatgtc gcgctcatac gtgtttgtga
      241 gttcaagaat agcgtcgtgc tggttgctat ctttcacttc ctcgagaatc gaggacattt
      301 tgaggctctg tatgagaccc cggtaacgct ctccgttgcg ctgcatttcg cgctcagccg
      361 tcacgaggaa cccgtgcagt tctgattcgc tcatgtcagg ttcaacgtcc atcgctgcaa
      421 gccaggcagg cagcttcagt ggcgacgggt ggacacgccg ctgtccccgt gattcggggg
      481 cctgttctgc attcgtctcg ctcgtgatcg agaagagcct gcgcgccagt gcggcttctt
      541 cacggccgag cgcaatgggc acaatttcaa tacggcgagt gcgcaggtta aagcgagtcg
      601 cgtaacgcct gccctgcgag tcgatataac gattctcaat actgcgcaag gtaatgcgcg
      661 aggtgtctat atcggcaaca gtctggatgc ggatatagtt gtgtcgcatc aggcgtgaat
      721 ttcttcgcgc aggcggcgtg ccttggcatt cagcgggtcg atggtgagaa tttcatcgag
      781 caccgatata gcccgctccc gctgccccat cgcatggtag atttcagcca gcaggttcag
      841 gttcatcacg ttgggggagc tttcgccgtt acgaaccttg cccgcatacg acagcgcctt
      901 ttcgaggttg ttcagtttac gttcagcaaa agctgcgagg tacataacct cctggtcatc
      961 cggctgaacc ttcagcagct tttcggctat attgctggca atttcgtact ctttgatctg
     1021 catcgcgagg ttgagtgccg cgcgcagcgc acgcggaccc gcgctcgatg aatggtacgc
     1081 gcggcgcagc aaggacgctg cctcatcgcg gcggccggaa tcaaccaggt gcttggcgtc
     1141 gcgcagcaac accgaagctg tctgtttttc tttcacgctg ccggcttcgc gctgcgcatt
     1201 gtactgcacc cgcacgagag agaaatcgtc ggtaatttca ccggtcgcgg caataagatc
     1261 gtagatttcg ctgaggcgcg catcggcggt ctcgacgtgc ttgagaaaca ggctctcgtc
     1321 ctcgttgata acccgcaccc cggcttcggt cgaaggcagc gcgacgtcat cgcggccgtc
     1381 agagcccagt agcacgatat ctccgggctt gagttgaatc tggtcgatcg caatatcgtc
     1441 ctgcaggcct ggcgtaccca acttgcgata gtagctgcgg ttttgcagga agatcgcctt
     1501 gccttcgcga tagagcacac cccacggatg ttccgcgttg aggtagtaca tcatacccga
     1561 attgttctcg agcgccgcga tgtaaacaga caccagcatc gaacagtcga agctctcgaa
     1621 taccttctgc agttcgagaa aggtattctt cagccatgtc tggggatcaa tactcgcctg
     1681 caggcgtgtg cgctcgatga ttgactggaa aacagcgccc agtaccaacg aacctcccgc
     1741 gccctgcatg gatttaccca tcgcgtcggc attgagaaag acggtcgccg ggcccgacga
     1801 aaactgcaca gtatgcgcga tggaaatatc gccgccgatg gtcttcttct ggttcttgaa
     1861 ttcaaaattc ttcttttgct tggtgaaaaa atctacatca acattcaggc cttcgtctac
     1921 agcattgact cctaatgggt tgagcagcag cgatgtcagg taatagtcgc cgtcctgctg
     1981 ggtcttgagc tcattgacaa gatcgaggct ggttttaagt tttttcgaag ctgttaccac
     2041 ctgttctttt tcttcaagaa ccgtgagctc tgcattgatc agttcaaccc ggcgctcgaa
     2101 atctttaaga cagagaaaag tgagtacgcc gacataaggc acaaccatgc caccaaggat
     2161 gatcgacccc aaatcagcgg caccaaaaaa gaagtacaac tgtataggca gcagccaggg
     2221 aacggaaaag aaaagaaagg taaagcggtt gcaggcaaaa gtaaaggcag cggcgccggt
     2281 aacaccgacg atcaggctga atgccgtgag cttgatactt tggttcttga tctctggcat
     2341 ctggaaaacg aagccgatac caatcactgc tgcgataatt cccgcctgca ggttcgcaaa
     2401 ctgaacacgg cgcggtacac ttttgatcca gttttcgcca aaaaaataga acaccatgat
     2461 cgggacgccg aaggaaaact ggataatgaa ccaaaaaaga ttatgctgaa tgccgctgtc
     2521 gatcatcagc atcacgtagt agagaagcat cgcacccatg agggcaacaa tcgccagcct
     2581 aacgttccag aacatctcgc gcgcaagttc gccgtgcaca tgcgtgacga actcggnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgt tccagaacat ctcgcgcgca
     2761 agttcgccgt gcacatgcgt gacgaactcg ggtttgacca ttttttcgac gggaagcgcg
     2821 aatattctca tgaatgctcc taaatcacat tatcggcaag tggcggctga tttgcaagga
     2881 gaaagctagc gcgtgaccct gtaaagcaaa aatgaacatt tgttcaccgt tgcaattgag
     2941 gatcgggatt gagcaaggct tttgcgaccc ctgaaacgct gaggcccgca ggaaggttga
     3001 ttaacctttc tttttgtgat ctgcttcggt aatgactttg atgttcttct taaaattcca
     3061 cttgcggtac accgaaagag catcgatgcg agcgttcggc agaaagaagt agtttgaacc
     3121 atactgcact atggcgcctt tgtagtcgga gtgcttgccg cgaaagtcga ccttgccgat
     3181 ttctttgacc tgcgtccagt tttcaacgtt cttgaggact ggcatctggt acaggtgcag
     3241 ctcccggatg ctgccttcct gcagcgagcg aatcattatc tgcgaccaat ccattgatat
     3301 agtctcggag cacgtgaaca tgaagtcaag ttttttagga atgccaattt gtcagatcca
     3361 ggtgcctcag aatgtaaccc cgcctgcgag gcgcagataa aagtcgaaac gctcgcgccc
     3421 ccgcacatct tctgagtagg tcactgccac gaacccctgc acagcttcgg tgtcgacgag
     3481 ggccatcagc ttgaacccgc tgttgcgcag gcgcatcgca gtcgactgca atagcactga
     3541 agaataccag tcagcgggga aaataatagg ttcgagcggt tgctccgaga aaagttcata
     3601 atgcccgcca ccgacgaaaa ccgcgtcggc aaaacccagc ccaaaaagac tctgtataga
     3661 agtgtcatgt cctaccgtgg cgccggcgcg cacatgcagg ccacgggcat cgctgcgtac
     3721 cgaaagaccg gcaagctgca tgcgctcttc gtagacgacg acacccgaaa gcaggtcacc
     3781 cccatagcgc agcataccga agcgtgaatc ggtcgagaaa aactgcgcaa accagttgcg
     3841 ctcgaagaac tgcagggcga gaccttcacg ctgctcgaaa attccactcg ccagcggact
     3901 agcctgctgc cggcgaaaga acagaccgcg tgcagagaaa tctccgcggt agcgggcatg
     3961 cagcaggtag ttaccttcac cgaccatctc gaagccggta ctgaaccccg tacgatcggc
     4021 tgcgcgaccc agtgccagcg acgcgatggg caactgctgt gcgccttcat gcatttcggg
     4081 taccgctacc ccgccggtca ggcgcaacca gtcgagtggt gtaaggtaga tgcgcgccgc
     4141 ggccaggttg ttctggtcca gctggtagta ctgcagatca gcgcccgcat aacgcagctc
     4201 ataacccgtg cgaatacccg tgggctcagc aaaggcgaag ttgcgcgtcc acggcagcca
     4261 cgcaccattg ccaaccagga agccgctctc aagcgaacgt tcgcgctggc ccacttcgac
     4321 gagcagcggg ccctgcgctg aactgtaaga tgcgacaacc ccctgcgcgg cgggtgcagt
     4381 gaaagtgcca atgcgaaaac cctcgtggcg gtagaccagc gcgccatagt cagctgtggg
     4441 agcgatagcg tttctgagta cgccaccctg gaactcgatg cggtcactac gcaccggcat
     4501 gaccacgaga agaaacagca gcgctctacg catcgcggca tccccagttc ctggcagcca
     4561 gggcacgctg tgccttttcg cttaatgtgc aggcatcttt ttcacgcgtg aatttcagcg
     4621 cctcgactgg cgagaggccc cacttgacga gtaccgcaaa cgccggcaca ttctttggct
     4681 tgcgccgctg ccactttttg ggctcatcgt cttcctctgg tggttcggcg tttgcgtggt
     4741 atcggggcag ctccccctgt gcctgtgtcg ctgtgtcgaa ttgaaaagag agcgccagtt
     4801 cccagaacgt ttcaccggcg ccgaaacgcg agagcgcgct gatacgcagc gctttgaccg
     4861 gtgccagcca gagtcccgcg cgcacgcgct gcgaaagagg ttcgaaaccg gcaatcgctc
     4921 cgccgtagcg cgagataaag aagaaagcgt tcgccgacca gcggtacggc tgtgagtgca
     4981 ggtattcaaa atcggagttg agctcaacgg gcaggcccag gtcgacgaga ccggaaagcg
     5041 aaacgcgcgt caggtattgc gaaccgctga tgtcggcgcg aaactgcggg tgtgaccaga
     5101 aaaatccgac tgccgggtag aagtctccct gcccggcgcg tatgcgctgt aacttttcct
     5161 gccggtagtg gctgccgagc ccaaaggcaa atcggtggtc ttcgaccgca aacccgttat
     5221 aaaccagggc cgccgcgttg agcagacttc gatcgccggc aaagtcgtca cgccgcgcgt
     5281 acaggttata gctgatactg ccataagaac ccgcagcctc aatctggtca cccgtaaccg
     5341 ggctgcgcgc ggccgcatta ccggtgtcgc gctgcaaccg cgccgaaaga gaatagacaa
     5401 tctgcggcag cagcagtgtt gccgcgaaca agcaggcttt cgccatagta actccacacg
     5461 ctttggggaa tttcccctct acctattaaa taccaggaaa ccgtgttttt ttctcacgac
     5521 cttcgctctt tttttcgcaa atgtggattt tttctggaac aggtagctac tggctaatta
     5581 tgtctcatct ttctgctatg gaattccaga tccctggcag gtaaatttcc cgggtgccag
     5641 tttttgctcg gcttgctacg gacagctgcg acaaatgtct tgacggtgtc tttttggcgg
     5701 ggtggttagc agcgtggtca agcgactacg cctcgaggtg ccggcgaagc gggtgaaaga
     5761 acccttcttg taccgcatgg tgaccgaata taacctgcag ccgaatattc ttgaagccaa
     5821 cctaagcccc gaccgctgcg ggtcgatgct gctcgaactc aagggtaaac cgcaggatat
     5881 cgaaaaagga attctcttct tgcgcgaagc cgatatcgaa gtcgcggccg acgattgatc
     5941 agatcgcggc tacaccgagc ttggcaaaaa gaaagctgat cacactcagc accaggaacc
     6001 ccgccaatcc catgagcacc cacaccagca ggtgacccag ctggaacagg cgatagttgc
     6061 tgtcgctgat gttgagcagc gcgcgtgcgc cggtgaacat ttgccggtag tgccagaaga
     6121 tgccaagcgc aagcagcggc ttgccaacca ccggtagaaa cgctgcgacc gaaagaaagc
     6181 ttaagatcag cgtcgaaaaa agcgcgatgc gcattccgct ctgcggtaca aaccggccgc
     6241 ccgagtgctc ggccacctct tcggcaatga cgccgatcag aaaaatataa cccgcataga
     6301 gcccgagcag cgcgggaaac accaccgcaa cgtggcgcaa gaacaaccgg atgttcacaa
     6361 agtggattgc ctcctgcact acgaaaaaga aggcaagggg cagcagcagc ggcaggtggt
     6421 acttacgaaa gagtgaaaag aaagaaccgg cttcttcgcg gtcgatcgtc tcccaggtct
     6481 cgggcgtatt gcgcagcaac tgcctcaggc ggagcgataa tagttcaaag cctgcacgct
     6541 gttcgagcgg cacaccgcct aacctgacgc ggaagtcagg agccgactgc gccgctttga
     6601 ctttcggttc ggggctcgtg aagaattgtt tgcccgaacc gccacgcacc gctgcgctct
     6661 tgcggggctg ttcgtgtttt ttttgcggca ggttcttcat tcgtcgtcgg cgttacgttt
     6721 cttctttttc aggcgcttct ttttcttagc aggtttcggc tcttcgccgt cgccagctgc
     6781 ggttcgccga tggttgagca gagcattggc acggtattcg atgccctttt ctttgtaaac
     6841 gatttccagt acgctgagcc gcttcttcga gggcttcacg gttacattca ggttgccggt
     6901 cttcacgtca taattgaagg tctgcggcgt cgagaagacg cgcacgcgaa agccgttgcg
     6961 atcaagcccc gccggaaccc tgagcgccag ctctgtttcg cccgatatca ccgcgccggg
     7021 ttcgctgttc aacaggcgca gcggcaacga acgcgcaagc gtcttctgcg cgaagatctt
     7081 ctcgttgtcg cctttaacga tcaggtagcg acgcagcagg tacgggtgct caccgacctg
     7141 gttggcaccg tggaaggtag taccggcaga cacataacct gcctcgcgca aaagtttgat
     7201 gctcgaggcg ttatagatgc caaacgggta agccaggtga cgcacattcg ctccgccttt
     7261 ttcgttgagc attttttttg aatcaacaaa ctgcgcacgc tgctctgcgt cggtcatcgt
     7321 gctcagcatc gggtgccaca tcgtgtggct ttcgatcgcg tggccggctt ttcgggctgc
     7381 ctgtatctcg gcccaggtca tcgaacgctc tttgcgcgcc atgatgtacg tcgggtagat
     7441 ataaagaact cccttgaact tatgcgcttc gagcaccttc tgtgcatggt tgataaagtc
     7501 gttattgccg tcatcgaagg tgatcataat cggccggcgg ccattggaaa aatcgagttg
     7561 cttttcaccc ttgacgtaag ccagcagctc gtcgggcagc accgtataga aattctggtc
     7621 ggcaagccac tgcatctgcg ccttgaactg cgcaaccgtg accgcatagg gcgtcgggcg
     7681 ctcgcggtac tgcgcttcgg tcacaatctg gtggtagcag agaacgggca caccgagatc
     7741 ttcgcgccat tgggcgggaa tctctgacgc aaccacgggt ggtatcaccg cgcttgccgc
     7801 agcggctgta tctgccggcc gctcttctgt gaccgagatc aggcggttca catcttctga
     7861 agcactcgca ccgtgtgcag gtgccggccc aggagtcggc tctgccggtg ccgttcggct
     7921 ctggaaccag gccagcacgc cgatcaccgt gaagaccagc cctgtcagaa agaggtacgc
     7981 tttgaagcgc gagtacatgg gcgcctatct ggcagtggca gacagggtgg aaaccagatc
     8041 gcaattacac tcgacggcgt cactcgtgag cagggtgcgc tttcttatgc cccaacttcg
     8101 gtcaaaaact tcgacgcacg gcaggaacat ggcaggcgcc cgcgcactct ggcgtgccac
     8161 cggcatgaaa gaagccgact tcggtaagcc gatcattgcg atcgcgaact ccttcacgca
     8221 attcgtgccc gggcatgtgc acctgaaaga tctcgggcag ctggtcgccc gcgaggtcga
     8281 aaaggcgggc ggcgtcgcga aagaattcaa caccatcgcg gtcgacgacg gcatcgcgat
     8341 gggccacggg ggtatgctct attcgctgcc cagccgcgac ctgatcgccg actcggtcga
     8401 atacatggtc aatgcgcata ccgccgatgc actgatctgc atctccaact gcgacaagat
     8461 tacgccgggc atgctgatgg ccgcgatgcg cctcaatatt ccggcggtgt tcgtctcggg
     8521 cggccccatg gaggcaggta aggtcaactg ggaaggttcg gttcgtaaac tcgatctcgt
     8581 cgatgcgatg gtagaagcgg cgaacgataa ggtcagcgat gcagaggtcc aggccattga
     8641 gcgctccgcc tgcccgacgt gtggttcgtg ctcgggcatg ttcactgcga actcgatgaa
     8701 ctgcctcacc gaagcactgg gcctctcgct accgggtaac ggttcaaccc tcgcgacaca
     8761 cgccgaccga aaacagctgt ttctcaaggc tggccggctg gcggttgaac tcgcgaagcg
     8821 ttattacgaa caggacgacg catcggtact gccgcgcaac atcgctaatt tccaggcatt
     8881 cgaaaacgcg atgagcctcg acgtcgcgat gggtggttcg accaacacag tgctgcacat
     8941 tctcgcagcg gccaaagaag gtggcatcaa cttcaccctg cgtgatatcg accgtatctc
     9001 gcggcgtgtg ccgtgcgtgt gcaaggtagc tcccgcaacg cagcagtacc atatggaaga
     9061 cgtgcaccgc gcaggcggag tgatggcgat tctcggtgaa ctcgacaaag ccggactcat
     9121 caaccgcgac gtacccacgg tgcactcacc aagccttgcc gcagcacttg accagtggga
     9181 tgttacccgc ccgacggcaa gcgaagaagc gaaagcactt ttcacggcag cgcccggtgg
     9241 tgtgccaaca caagaagctt tctcgcagtc gcgccgctgg gaaacacccg atctcgaccg
     9301 cgagaaaggg tgtatccgtt cggtgtcaca tgcctattct aaagacggag gtctggcggt
     9361 tctgtttggc aacctcgcac cagaaggctg catcgtaaag actgcggggg tcgatgaatc
     9421 gatctggaaa ttttcgggta aagcaatcgt gatggaaagc caggaagagg ctgttagccg
     9481 cattctggcg aacgaagtta cggcgggcga tgtggttgtc gtgcgctacg aaggcccgaa
     9541 aggcggaccc ggcatgcagg aaatgctcta ccctacttcg tacattaaat cgaaggggct
     9601 tggaaaatcg tgcgcgcttc tgaccgacgg ccgcttttcg ggtggtactt cgggtctgtc
     9661 catcggccat gtctcgcccg aagcagcaga caggggattg attggcctgg tcgaagatgg
     9721 cgacgtgatt gaaatcgaca tccccaaccg ccgcatcgag ctcaaggttg cagattcggc
     9781 tatcgccgaa cgccgcaagg cgatggaagc gcgcggcaaa gacgcctgga aacctaagtc
     9841 acggcaacgg ccggtgtcag catcactgcg tgcctatgct gcgatgacca cctcggcgca
     9901 cacgggcgca gtgcgcgatg tgtcgcaggt agagcggcag ggctgaccca tttcactcct
     9961 ttttaaggag tagtaaaaaa gtgcttattt ttctggccgg cgcaaattgc gtgctgtata
    10021 atacatgaac tatgattcat gatataaaaa ctagtaaatt agctctgtcg ggcctcgtct
    10081 ggctgctggt ggccgtgaca tggggtgcga cagttacgat gctgttccgg cgcacagtac
    10141 ccggtgataa ggtagccaac ctgacgacaa atcccaatgg tttgagggtt tggttaccgg
    10201 ataacagcga aacagccgcc gggaatacaa atagctttgt ccactcacag aataaagctg
    10261 tagtcaaccg cggggaaggc aagaacggca tataacatct gcgcgcacgg actcgaccct
    10321 gctgcagtgg cgctggtgca ttgctagaga cgctaaggat ggcgtctcca gaaaaaaggc
    10381 tttctgcgaa accctagccc gtcagcctga atgtccccag aaactccggc aggtcgtgca
    10441 tgtcgaagtg cgtgaccgac tttgaaatga ctttgccggt acgcaggttc ttcacctcgc
    10501 cgaagcaggt ggtgtgtgag tacggcaggt tccagggata gatctgctgt ttttcgccca
    10561 cgtactggta cggctcaccg tcgacagtaa aatcaaatgt ataacggatc ttctggtcct
    10621 gcaggtaacg cagctgcagc gtgccgtaga ccggcatgtt ctcgcacatg ccgccgatcg
    10681 tcacggtgcc cgcgagttcg ctgagcatga acttgtcggc tgcagggttc atgaagtcga
    10741 cgaggttctt cgggccccaa tctgccttga attcaaactt gtgttttccc gcggggccaa
    10801 aaccatcgac gaactcgtgc tcgccggaca tgacttcgtg gatctggaac cctttttcgg
    10861 gtacaccctt ctttgccgta tcgagtatcg aattcacaat gcctgcaacc tggtcgacca
    10921 cgccgccgat tccgagcgat tcctgctgct gcttcgacat gaaagtgtgc gtcgtcttgt
    10981 ccatgatgcc cgcggcagaa ggcagcacac ggatatactt aaagaactgg ttgagtatgc
    11041 tcaccatctc gacgtctttt tcttcgcgca ccgaatcgaa gatcatgcgc gcgacggttt
    11101 cgggtttgtc tgagtagagc ggcatgaagg caaagaaaag tttttcgcca atcgattcgg
    11161 cgctggtcgt atcatagaaa ccggtgttca ccaggaacgg ataaaccgtg gtcaccttga
    11221 tgttatcgcg tgccacttca acgttgagga tttcagacat cgccccgacg gcaagcttcg
    11281 tcaccgaata agctccccag gtgggagctc ggaagtatgc ctggccgctc gccacgttga
    11341 cgatatggcc cgacttcctt gccttcatct gcggaatgaa tgcctgtatg tggtaaagcg
    11401 gcgcccagaa gttgatgttc atgagctgct gccacttgct cagcggagtg tcgacgagtt
    11461 cggcgttgaa gccaataccc gcgttgttga tcaggatatc gacgccaccg aatttctgct
    11521 gcacgtcgtc gcgtagcgct tcgacggcct tgagatcggt cacgtcgacg accttggtca
    11581 gaatatcgac gccgtgtttt gcgaggcgct gcttcgcatt ttcgagcgca agttcgttga
    11641 tgtcggtcag caccagccgg ctgcccgctt cggcaaaaaa tccggcagta ttgaggccga
    11701 taccgcccgc agcaccggta atcagcacga ctttattcct gaggtctttc atagtactaa
    11761 attacccaaa ggcgaacggg gtgtcaataa aaaactgaca tcagtgtcag tatgtctagc
    11821 gggtcttttt tgcggtgaaa gcacctttct tcttattcca tagatattga acctttgagg
    11881 taaagtgtat ggattgaaag attttttcct gcagatcggt gttattgtcc agcttgccag
    11941 ccatggtctg ctgcacctgg aagcgattca ggtttccgct gcaataagcc agaatcgttg
    12001 tacccttcct gggaagttcg aaagtgggca agatcggatc agggtcaagc ccggaaagca
    12061 ttggattctt atagaaacct tttatggtcg ctgccgaggc aaggtcgcgg cagacgggtt
    12121 tgaccaaagg aggctcgata ggctgcaact ttgttgcctg tagttgatag aatttgattt
    12181 cactcacata ggtatcacga tcgtcctgaa agttatgcac gagtatggca cctgcaccat
    12241 ctgggtaata aacggcaaac tcgtggagga tttttccgcc gcctgtgccc gaatcaagaa
    12301 acgctgcata tccgttcgtc gcgtctacaa ccgctggatc catactctcg ccaacccacg
    12361 tgtctaccca accgccgctt tgcctgagca gagatcccgg tttcaagagg ttaatcagtt
    12421 caacgatgtc ggggcgtgct ttatctgccc acaagcccga agctggcatc agttggcaga
    12481 gtaagctgca accggccagg aacagcagaa gcggctttac agatttcatc ctgccagtag
    12541 cgggcaactg agaaataccg taaacaagaa tcgcagcgtc aggaaaaaac cggcccgaga
    12601 tagaaagcgg gttgttcaat gggttgctgc ctcatcgatt ttggtgctgg tgcgatcgct
    12661 gtcttggcag cctcgacagg caaggattcg ctcagtggct ggtcgatttc aatatcctgt
    12721 tctaccgtgt agtcttcacc gccaaaactc tgcgcaatac tatagagcag gttgaattcg
    12781 cgtacggtgt acactgaatt gccgatgctg ttctttacct cttcgaggcg cgcaagcaga
    12841 tgatcgtgca gcccaagcat gacagacttt ctgctgatca ggctatataa gaaactccag
    12901 taccgcgcat attctgtgta caggcagtaa aagacacgcc ggtactcgac gccgtctgta
    12961 ggctcgggtg ctctgtcata tttgctataa ccctcataga tcgcaaattc ttcgaaatcg
    13021 gtcacgataa cgaggcggat ttttgtgtcg tgcgcatatt tgcgtgcctg gtaaaccggg
    13081 tatgcatcgt cggcaatgtc tgccgccggg cgcttggcct cgatgatgaa cttgtagctg
    13141 tcgcctactt tgaaggcgta atccacatgg cgtttgttgc caatgtgtaa ttcgtgaatg
    13201 acgtctttgt aatcctgtgc gtatcccgcc tcgttgtcga catcccagcc caacagccgc
    13261 agcaacggat tgatgaattc aacgcgcacc tgcgtttcga gaaattcagc agacttgtag
    13321 tgcgcctcgt tgatctggaa tttagcaacc aactcccgta atttttccgg gacacgcggc
    13381 ttcagcatgg gtaattgtaa ccatggggtt ctatcgcgtc aaatcgaatc gatttgttga
    13441 cccgccagtg cgcgggcaca tgcacggtca tggatctcaa ctgccggcgc tgccataaac
    13501 ccattgccgt ggagaatatt aacatccagc agatgatcgc caagtgcggt gggtgcggtg
    13561 cagtcttcac cttcggccca gccgatgcct caagagaaac ccactcacca ggcggcacga
    13621 aggctacagt gccccggccc ggcagctggc aggtagcacg cgagggcaat caactggtct
    13681 taacccgcaa gtggtttaag ccactgttcc tgttcctgct gctgttttcg tttgtgtgga
    13741 attcgtttct cgtgggttgg tactcgctgc tcggaggtat cggcggcgaa gcgggcttct
    13801 ctgccttctc gctgatcttc tttattttcc ctttgggcca tgtcgcggtt ggcctggggc
    13861 ttgcctacta caccctcgcc ggcctgctca atcgaacgat catcaccgtc gatcgcagcg
    13921 ccctgtcgat aaaacaccgg ccattgccgt ggcgccacag cggcatgatc gccgcgcacg
    13981 agcttgacca gctctactgc aaagaagagc gccagtcgaa caaaggcagc acgtggtaca
    14041 cgtacacggt gagcgcgctc ttgcgcgacg gccgcgagcg taagattatg agtggcctcg
    14101 attcacccga agaggcgctg tttctcgaaa gcgaagtcga gaagtatctc ggcatcaccg
    14161 acaggccggt ggcgggtgaa tatagggtgt gacagactaa tttaacgtaa gtcctgtgcg
    14221 cctttccgcc actcaggatt tttttcgagt tgctggtgtt catcgtgcag ctgcttcaag
    14281 ggctctttcg ccagatcaac ccggcttttc tttacttctg caccgtcgta tgtgagcgca
    14341 gcgcctgcgt ttagttctat atcattcaag aaatcctgca tcatcagatt cggatctgtc
    14401 tgcaaaccgg tttcgcgcac aaagtctgtc atgatcagtt tgccggtgaa gaggacaatc
    14461 cggaccgacg acttgcgggc ttcaaccgtg aaaccagtcc cgcgcacccc cgcggtcact
    14521 acaggcgttt tgattgtcca gcggttactg ttgtcgcgac cctctttgcg aaacagcata
    14581 ctcactgcac cggtcgcctg gtagtaattt gaatctggcc gaatctcaag cgtcgaaaat
    14641 ggattcatga aaaatgaagc tccgtcagac agttggacac cgacctttgt tcttgctgag
    14701 gtcacaatta catcgccttc gtttatcgtc gcaccaagct tcagtggctg cgacttgcct
    14761 ttgctcctga cctctgcctg acctgaaagg taggtaatcc gtccttcgac tgcagataga
    14821 gcctgaaagg ccagcaagag atatagccca gtagttttca tcgctctctc tcaataatga
    14881 tagcgcgcag aaagcgcagc aaagaatgta tcaagcttga acgcattggc gttcatcgca
    14941 aaatccagat ttactttctc tgccggcacc cagcccaatc ccattgcgac agtcaactcg
    15001 tcatcttgat aaaaggcacg actggtttca attgtctgcg ccgtggtctt gtcggtggtg
    15061 gtacgcgacg aaaaaacgtt cttccgaaca ccgaatcggg tctttagcgt ctcgaatgtc
    15121 gcatgctcca ccgccactcc aaccggcaca aacagatggt tcttatcgta gctgttattc
    15181 agtgcggctg ggttgttgtt atccacaact gtgtatttga cggatgtatg tccgattccg
    15241 gtcgaataaa tgaccttgag tttaccgtcc tgaaagttct ggtggtaggc ggcgtccagc
    15301 tgcagattga gcgccgaagc tgccgcatcc tgcgatttta ccgatcccgc aagatttgta
    15361 ccgcggatct catatggcat cttgtactgg tcgatattcg ctgccaccaa cagcctgtct
    15421 tggcccactg gcgtcacata gcggcccaaa agtccgatcg aaggtgctgc cgagctcctc
    15481 acagtaagat tctcggctcc tgtagtcgtg gtcgcattgt actccatgtt catcaggggc
    15541 aaatcgctcg agagggagag atccagatag cccggcccaa ggttcttcca ctgtgcgccg
    15601 actccaacac cgatattgtg ggcgctcata gtgtattttc cattcacgcc ggtcgctgcg
    15661 actgactggt cgacgttatt gcgcatgtta gcataactga ctcgcagacc aaggttaagg
    15721 tccccgagcg ccatgccata catcaaatcc aagttaccga accccttggt ggtcttgttg
    15781 agaattgcga gatcggcgcc ggtgaagctg ccgtcaaagc cggttaaacc gttcgtcagg
    15841 tggccggttt gggcatattt gtcgaagagc gcctgcgtcg atcccagggc gctgtttgcg
    15901 ttgagaggcc ggcgaacaaa aacagccagc ttttggtttg ccggcagagg aactgttgca
    15961 ccaccccaca caagattcgc cccgagccaa ccttcgaggt atgcggtgcc ataattcatc
    16021 aactggcccg gattcatgaa catgttcata tctgattcaa tgagcctgtt gggaacagca
    16081 aggccacgcg tttgagtgtc gagcggctga ttgacgccgt tgaggtccga gaggtagcag
    16141 acgtcggaag agcccggtgc acaaaccgtc acgtctgtgc ccgtattggg tggcaccgca
    16201 tatacggcaa acggtatggc agcaagcaga atcgcgacat ataggatgaa tctcatatgc
    16261 tcccgaaaaa atctacccga cgccctgcgt caagttggaa aattattgag gcaatggaat
    16321 acgatctgtc tcgccagcaa cgatacccat cgtgttcgcc tgccactcgg ccttcgcgcg
    16381 ttcgatgcgt tctttggatg tcgcgacaaa attccaccac aggtggcgcg gttctgcaaa
    16441 tttttcacca cctaagagta ctgcgcgcgc agcagagtgg gagcttaact gtacacgcgt
    16501 ctcgcccgtg agaacaatca tagctcccac ttgtgctgtg tgaccgaacg ccgtgaagct
    16561 gccgcgcgaa acgtacagcg ctgcctcttc gccgggtggc aactcgagct caatgatggc
    16621 agcgccagtc gtctcgaggt cagcatagaa cagtggcgaa tgcgttttca ccggcgacac
    16681 cttacccatc agcgtaccgg caatcaggcg gagcttccac tgctcgcctt ctattaccgg
    16741 aatgacatct gaccggtagt gctcaaacgt cggtgcagtg tcttcgtgtt caaccggtag
    16801 tgcaacccag gtctgtatac cttcgatctt ggcaggtaag ttctcttttg ttgaatgctc
    16861 gctgtgcacg atgccgcgtc ccgcagtcat ccagttcact tcgcccgggc gaataacgag
    16921 ctcggtaccc aggctatcgc ggtgcagtac tgcaccgtca tagagatagg tgatcgtcga
    16981 aagcccaatg tgcgggtgag ggcgcaccgt gagctcatca ccggtaacga ccggtgcggg
    17041 accaaaatgg tcaacgaaca cgaacgggcc aacgctacgt gcatctacat gggggagtat
    17101 gcgcagcaca ttaaacccgt cgccgaggtc tttggttttg ccctggataa ggtgtgacat
    17161 aattattcac gaaagcttac agccatcggt cccgaaatgc caaaaattgt tgcactttca
    17221 ggcatggcag acgacaggac attaagccta tcatgcaacc gacccagttt gaagacaccc
    17281 cgctaaaaga aagcctgaaa aaattctccg gtatctgcct ggctttagca tttttgattt
    17341 cgctttcagg tctttgtggc tggtcgtttg atataaggtg gctgcgcagg ccattacccc
    17401 agtttgttgc gatcaacccg ctaaccgctg tcctgctcct gttggctgcc gtggcacagc
    17461 tactggcaat caggcgaacg cgaaataata gtgcaatcgt agcgtccatt ggcatgataa
    17521 tcactggcac agcactcgca aaactcgtgg atcttttttc acccggaatt gcgccggtcg
    17581 accaactgct atttgcacag gcagtcgatc aggagcgaat tgccaatttg ccaaaccgca
    17641 tggcacccaa tacagctttc tgcttcttgt ggctggggtt cgccaccata ctacagccgc
    17701 ttggccaaaa gggaaaattc gtaccggcgc agtttgcgct tatccccctg gtgcttgcag
    17761 ctttgctgag catactgggc tacctgtacc gtgtaccaaa attttacggc atcatgaact
    17821 atattccgat ggcgctgcac actgcggcgg gttttttatc gctggcgatc gcgcatatca
    17881 ctgcaactcc ggaacaaggt atcctcaggc attttaccac taaccttgcg ggcagccggc
    17941 ttgcgcgctg gcttgtgccg gcaacgatct ttgctcctat attgctcggg tttcttcggc
    18001 tgctcatcgg ctggcagtat ggcgtaccgg tggagtttgg tgtcgcgata ctggtgttaa
    18061 gtattatcac gatactgacc ttgttcgtct ggttcacaac ccgtgcttta aacctgaaag
    18121 actatgaaca gaacatggtg cgcgctgagc tggcgcaggt gaatgcccac ctggaaaaaa
    18181 ccgtaaatga acgcactgcc gccgctttaa aaacagaaac ccgcctgcgc caggttctcg
    18241 acgctatgag cgaaggtttc cagatcatcg gttttgactg gcggtacttg tacgtcaacg
    18301 atgcggttgc acgacaggcg aaagtcccca aggaagtgct tctccgctcg acatttctgg
    18361 agctctttcc caccagcgtg aacgcagaac tcatggaggc tttccgtcgc tgcttcaaga
    18421 atcgtgaggc gctgcacatg gaaaaccgct ttgtctttcc ggatggcagc gacggctggt
    18481 ttgacctgca ctttcaaccg gtacccgagg gattgtttat tctctcgtct gacattaccc
    18541 atcgcaagct tgcagaaatc gaaattctga aactgaacga agaactggaa gaaaaaatcc
    18601 gcgcgaggac agcgcaactg caggaagtaa atgccaatct ggaacacctc gcacaggagc
    18661 gggcaaccgc tgtactgcag accgaaagcc gcttacaaca ggttctggat aatatgctcg
    18721 aagggattca aatcatcgat cgcgagtggc gatatgttta tgtaaatgaa tctctgctga
    18781 aacaggcgga aaaggcacgc gaagaacttg tcggctttcg ggtaatggat ctataccccg
    18841 gggttgagca gagtgaactg ttcgcaacac tagaacggtc catgaacgag cgcagagctc
    18901 agcaaattga aactgcattt cggcattcag atgagcgttc tggaatctac gagatgagta
    18961 tccagccgat accagaaggt atttttattc ttaccgttga tatcaccgag cgcaggcgcg
    19021 ctgaagagca gctgcgggag gccaacgaga gactcgaatc gaaggtgcgc gacagaaccc
    19081 gccagctgaa ggagctgaac caggaactcg aatctttttc gtattcagtc tctcatgacc
    19141 taagggcacc cctgagaacg ctgactgcgt atacccagct tctgcaaaga ctctcgggca
    19201 caaatctcag cgaagaatgt aaggatatcc tggttgtgat tgaaaagaaa gtcgaacaaa
    19261 tgaaccagct gatctctgat ttgctggcat tttcgcaact gggttcgcaa aagattcatc
    19321 cggcccctat cgacatggac cttctggtgg cagatgtgct gcgtgaactt tttcctgaag
    19381 gcatcccagc aaaccttgta ctgaacattg ataaactgga ggcagcttac ggcgatgcaa
    19441 atcttataca gcaggtaatt gtcaacctgg tttctaatgc ggtcaagtat tctgccaggc
    19501 aggaacaacc taccttgaaa attcactctt ctgtcgttga gggcgaggta attttcgaaa
    19561 ttcgagataa cggcgtcggt ttcgacatga cctatgccga tagactgttc ggcgtcttcc
    19621 aaagattgca tagttccacc gattttgaag gcaccggagt tgggctggca attgtgcaga
    19681 gaatcataca caagcacggg ggcagggtct gggcagaggc agcacccggc aagggtgctc
    19741 gcttctactt cacactaccc tccgttaagg tagcgaaaag ttgaccccgt gcaacggttg
    19801 aggacgatac tatagtaagg ccactatgcg accgctcatt tgcttattgc ccgctttact
    19861 gatctgtgct ttgcatcctg catttgcaca ggaaaaaccc ctgccggtac tgcaatacgt
    19921 cgatggcaac ggaaactcct atgagtttac cgaagagggg tcgggctacc tgctcgaatt
    19981 tgaaccagtc aaaaaaggca agtattcggg tggcgaaccg gcaagccgcc gcgtcagcaa
    20041 agaaaactac gaacgcctgc tggcgcgcat ggatgaaacc ataaagaaga aacgcctcac
    20101 atctacccga gaacgcggca cggcgctcat tatcaagctg gtaaagggcg acgttaaaga
    20161 atatatcctt ccgaagggca gcaaagacat caaagagatc gaagctctgc tttacacact
    20221 gttttgagcg ccgcacgcgc acgcgtggcc tcaccgtttc tcaccgcacg ctggaaaaat
    20281 ctgttcatgg caaactacga ggtcgatgcc ggccttttgc agccacttgt gcccacaggc
    20341 acggagctcg acctgtacca ggggcgtgct ctcgtttcgc tcgtcgcctt ccagttcctg
    20401 aacacgcgcg tgaaaggctt tcgcatcccg gggcatgtga actttcctga aattaacctg
    20461 cgctactacc tcaagcgcac cgtggagggt gaaacgcggc gcggggtcgg cttcgtgcgc
    20521 gaaattgttc cgcgccgcgc gatcacgctg gtggcaaaca cgctcttcaa cgaaaactat
    20581 gccacgcgca agatgggcca gcgggtgcgc gtcagcggcg acagcctgaa agctgaatac
    20641 acttttgtcg aaaaaggccg cgaacagaaa atcagttgcg tcaccggtat cactccacag
    20701 gcgctaaaac caggcagcct cgaggagttc atcactgaac actactacgg ctatacgaaa
    20761 ggccgcagga ctctcgaata ccgggtcgag cacccgcaat ggcgcacgct gcaggtgcag
    20821 gactacgcgg taggcgtcga ctttggtctc gcgtacggtg agcagtggtc aaaactgacg
    20881 ggcttacgcc cgcattcggt gcttttcgcc gaagggtctg aggtgaatgt attctctgcc
    20941 gggaaaatta tttacggctc atgagaatgg ggcaggcaga gatcatggat gtgcatgacg
    21001 cagtggtgct actgaaggca caagatctct ccgtagaaat tgcagcgctc gacgaactca
    21061 ctgccgacgt gcagaagaaa ctcgcgggca gcgaaatgcc gcaatggcac aaggatattc
    21121 tggacgatag actgtcaggt gatgaaaaat tcagcccctg ggaagttgta aaagcccgtc
    21181 tccaaaatgg cgctaaaggt tgaagtctct gcctcatgcg aaattgatct tgaacaggcc
    21241 taccaatggt atgaatcaca tcgtgcgcaa cttggattag aatttctgaa cgactttgac
    21301 gaacgcctga acctgatcgc cgtaaatcca tatatcggcg ctgaagttta tcgccaaacg
    21361 cgaaagttcg taatgaccag atttccctat atcatatatt accttctcac cgatgaccat
    21421 gttaaggtcg tagcctgtct gcatggaggc ttagacccgc gcctgagtgc gggtacggta
    21481 cacaacagga tcatcgagca atgaagaaaa agaacgcaaa gaacttcacc cccgccgact
    21541 atgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcctt tcatgaccgg
    21661 ctcgtgaagg cgggtcttgc cgaacccata taccacattg aacccaaatt cgacggcctc
    21721 gcgattgacc tcgtttacga gaatggcgtg ctcacggttg gctcgacacg tggcgacggc
    21781 gaaaccggcg aagatattac acacaatgtg cgtaccgtcg ccaatattcc gctgcggctc
    21841 gcgctcgaca aaccacccaa gcgcctgagc attcgcggcg aggtaatcat caagctcgcc
    21901 gacttccaca agctgaacga gcaacaggcc aaagatggca agaagctgtt cgccaacccg
    21961 cgcaataccg cggcgggcgc gctcaggcaa caggactcca gtgaagcgcg taataagaag
    22021 ttgtcttttt ttccttatac gcttgcacat tctcccgagg tgcagaaata tttcggcaaa
    22081 gatatgcgca cacaagaggc catctggaac aaggtctttc ccgcgctggg cttcaagatc
    22141 tctgcctacc accgcactgc agacattgaa ggcatcattg cctactacga agaaatgacg
    22201 gccaaacgcg cggacgttga atacgacctc gacgggctgg tggtcaaact caacgacacc
    22261 accgaatggg agaacttcgg cgcgaccacg aaggccccgc gctgggccat cgcgctgaag
    22321 tttcccgcgc ggtcgggtat cacaaaactc gaacgcgtcg tgtaccaggt ggggcgcacg
    22381 ggtgtcatta cccctgtcgc cgaacttacc cccatcaacc tcggcggtgt catggtctcg
    22441 cgtgcctcgc tgcacaacgc cgacgagatt gagcgcctgg acctgcacga gggcgactac
    22501 gtcgaggtag ttcgctcggg cgatgtaata cccaaggtgg tgggcgccaa acccgaacag
    22561 cgggctaaaa aagcgaagag gatcgccttc atcaagaaat gcccctcatg cggcaaagcg
    22621 ctcgagcgcg aagacgtttt tttccgctgc gtaaacgctg cctgccccga gatgaacgag
    22681 gcgtacctgc agttcttcgt ctcgaagaac ggtctcgata tcgaatcgct cggcagtgaa
    22741 tgggtggcaa agctctacca gaataaggtg atcgccgact atgccgacct gtttgcagtc
    22801 accaaagaag acctgctgca atttgaaggt atgggagaaa ttctggccac gaagatactg
    22861 cagtcgatcg acacacgccg ccaggtacct ttcacaaagc tgctgaccgc ggtcggaata
    22921 cccaacgtcg gggagcacgt cgcttcaatt cttgccgaac atttcgaagg tcccgatgcg
    22981 ctcgcgcagg caagcctcga agagctgacg cagattcacg aaatcggccc gggcaccgcc
    23041 gcctcggtgc aggcatggtt tgccgataag gttaacacat cgcggctgaa gaagctgcag
    23101 aagaacggcg tggagatact gcgggctaaa cgccaacggg tatcggacgt tttcgcgggc
    23161 aaaacctttg tttttacggg agcgctgagt caattcacgc gcgaaagcgc cgaagaggca
    23221 gtcaaggccc gcggcggccg cgcagcgtct tcagtgagta aaaaaaccga ctttgtcgtg
    23281 gtcggcgccg acgcaggcag taaggcgcaa aaggccaaag aactcggtgt aaccatcctg
    23341 agcgaagaag agttcgcaca gatgctctaa aatcgctgtt cgtgacattg cgctatgggc
    23401 tttcagccca cagcgcactg caaaaaatga aagcggttgt ttcagacttt actcgcgcca
    23461 tctgggaatc tctgggctgg gaagcctttg gtgtcgatga ccgtatagaa atcaaagatg
    23521 tcactcagga tagccggcag gtaaaaaaag gctcggcctt ttttgcctat gacggtcaca
    23581 aggtacgcgg taaagatttc atctgggatg cattcgaccg caacgccgcc gccgttttcg
    23641 tcgaccggga atacaaagaa gaaattttca acgactcccg ctacaataag aacctgccgc
    23701 tcttctttaa ccatgacttc cagcgcggcg ccggtcgcac gatctcgttt ctttatggtg
    23761 agcctacccg gtacatgtcg tgtatcggcg ttacgggcac gaacggcaaa accacgatag
    23821 cgcattctct ttaccaggca ctcagccacc tgaacaaacc ttcgatgtat atcggcacgc
    23881 tgggcatgga agtctcgcgg cataaatccc agacgggcat gacgacgccc gacacggtga
    23941 acctgcagca catgctgcac cagggccaga agcagaaggc gctgtacgcc tgcctcgaga
    24001 cgagttcgca cggcctcgcg cagggccgtc tcgaaggtat caatttccag atcggtatct
    24061 tcactaacct gacacaggac cacctcgact accaccgcaa catggaggcg tattaccgtg
    24121 cgaagcggcg cctgttcgag tacatgctgt ttgcctctcg caagcagccc gagaccgcca
    24181 agggatcgat tgtctgtatc gacgacgact acggccgccg gctgtaccac tggctcaagg
    24241 gccaaaaccc aagttttccg gttatctcgc tcagcacgcg cgacaagaat gcagacgctt
    24301 acatcaccga tgtcgaagcg acttttgatg gctaccggtg tgtcgtggtt tttgcacaaa
    24361 agagttactt tatcaagacg cgcctgttgg ggcgcttcaa cctggttaac ctggcatcgg
    24421 tattcctgag cctgctctgc ctcggctttg gcaccgacga agcgcttgaa gcgatacagc
    24481 aggtcgagcc agtacccggc cgctttgaag ttctgcgcgg ccttgaacaa cgcaccgtca
    24541 tcatcgatta cgcgcactca cccgacgcgc tcgcaaaggt gctcgagacc gcgcaggagc
    24601 ttaccccggc acgggtcatt ggtctcttcg gttgcggtgg cgaccgtgac aaagagaaga
    24661 gaccgcagat ggccgaggtc gccagcctgc atgctgacct gtgtattctc accaacgaca
    24721 atccgcgcag cgaagacccg aaaaacatca tcgaagacat gcgtaagggc ctaaagaccg
    24781 cacgctacct cgtgatcacc gaccgcgaga aagcgattcg tgcaggctta cagattctcg
    24841 gaccacgcca ggtgctcgtg attgcgggca aagggcatga agactaccag atcatcggca
    24901 acaagacgct gcatttttct gaccgtgaaa ctgcgct
//
LOCUS       DAOQJF010000005        23757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_940_length_23757_cov_4.977301, whole genome shotgun sequence.
ACCESSION   DAOQJF010000005 DAOQJF010000000
VERSION     DAOQJF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_940_length_23757_cov_4.977301"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..598)
                     /gene="bfr"
                     /locus_tag="PLF85_00575"
     CDS             complement(122..598)
                     /gene="bfr"
                     /locus_tag="PLF85_00575"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNE18174.1"
                     /translation="MKGDKKVIELLNANLKNELTGINQYFLHARILKNWGMEKLGAYE
                     YKESIDEMKHADKLIERILFLEGLPNLQDLGRLRIGETVEEILKGDLALEMEAVPLLK
                     SSIAHCESVNDYVTRELLAEILESEEEHVDFIETQLGLIDKMGLQNYVQLQAPSAE"
     gene            771..2840
                     /locus_tag="PLF85_00580"
     CDS             771..2840
                     /locus_tag="PLF85_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE18175.1"
                     /translation="MSKDAKDHALESATHAPWQREFFKNIDIFMQAGLKEEKARELLI
                     KYLKLSVDTPIPRVMETFKDESKLEEVGVFTQRDEKLRDFMIEFLTPMLKKFHIEGEE
                     NLDEILPLLGKYPVTLISNHLSHFDTAAIYGLLYNHSKEARKLADSMVFIAGRLVFLA
                     DFTRLGLYTIESLLVCSKRDMTDNPGMADMMTRINMRSFRQAADLQKQGKVICIFPEG
                     TRSRTGRLITFVDTVYHYVMNKVIVPISLVGTDKILPAESFLFQQATGKLVIGKPILV
                     GKLHGESAKTFPEHIQRLEIPEVENKKQFVIDSLALLIGQNLDRHMHGTYRNLYLDDG
                     KTKKKNILIKAAPTPHAEVGIIGHSPYATAMAAVLANKQVNIRVYLPDAEKARTFNEV
                     NTDLENFPIFKLPPNIAFTSDPKDLLKCSIYLQGVRPWELEKYYEQVTPVLNQSFATI
                     IGIVKGFTGSKYGLIFEDLEKIYKINPARFAVLSGANYPEQILERKPGGFELAAVNTS
                     LVSELIDFFTTGYVYTRPAVNPYDVRGLQIGGALKNIYALGVGLVDGYYEKQLGGNND
                     STLFHLAHRISAEMANMVVEMGGRKSTLAGVAGITDLMLSCFGQDSHDRQYGHDFVFD
                     THDKKKQLSGVYGIKALPRLVRLREEKYPIAKAIHDVIADGKDIDDAIERIAETFGQS
                     RAVQFRD"
     gene            2918..3607
                     /locus_tag="PLF85_00585"
     CDS             2918..3607
                     /locus_tag="PLF85_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18176.1"
                     /translation="MEYVAFLFLGLGVLLVLAGVLLQANEKRAGGRSPLEDYALQSAR
                     PETRANPLSALKTSAMPAVAAAAPVVQPAPAMSSAIPGPFQFVQHEPAAPVPVQAPAV
                     APVAVSVGQANPRLFEKFAYLYLDSSARNVYDGAGSVAAFDLSEVQGIRRFGRGLFSY
                     DGFGFYFDHASGQERFPLTNLKTIGFYPNCVALTFRTSQPVALLFMEETETIRKVLET
                     FRAEAAGNVSG"
     gene            3659..4705
                     /locus_tag="PLF85_00590"
     CDS             3659..4705
                     /locus_tag="PLF85_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18177.1"
                     /translation="MRAYDFSTHNKTVKRDKSRYFSYAFIFLLLLAGSAFVYYVILNS
                     PRILYYFRENKYSEIERAHAAAIKAIQVLPAEQAASAKNGALALAEVQDFIELAQALQ
                     KDHREDPILTYLEATVLDEIIRRQVAEKPEALLALLFREFIGRPAFPAEFDRELWQRA
                     LLAGRKARSLGLPEVLSARLAEAELDVYLLGGRPWWESAQEIAASNATAKKLPSWHLM
                     QAGMARETPDFALVQTAYGPTTAAFAKGVYYIRSGNSPLGISNFRDLSKSQADAFARD
                     HALYTLGFLSAREKRIRDQLSYYKQIRFPEFAPRYPFFVPELNYLLRFLGQQGEADQM
                     MKAWEELKAQEKSQ"
     gene            4748..5482
                     /locus_tag="PLF85_00595"
     CDS             4748..5482
                     /locus_tag="PLF85_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18178.1"
                     /translation="MDILANPYLNIVFNALISGGAFLCFVLGIAMLLLKYPRRSVSYL
                     ILLSFVMGITQLYAWFNLNTLFFRPQWLNYIFVGANFLIGPGVYYFYKSTGDDSFVAD
                     RRTQLAFLPGMFFSLLIPFINLVAPEVMPTDPRQFFYTGRPSFIDAIFSLALLHNAGY
                     YIKLFLVTRPLIVANRNMKETGALTAFLMFFGIITLINLYGIIAYLTRDIRHFYADSC
                     IITLLIVSFMVFALRYPQYFLRVRPE"
     gene            complement(5486..7846)
                     /locus_tag="PLF85_00600"
     CDS             complement(5486..7846)
                     /locus_tag="PLF85_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18179.1"
                     /translation="MKQLSLLLSLLPYTMLAGAELAPADRRLPHANWRITQADIGELP
                     SGGHVAGLLEFLYQPAVMANTDTGGFARAENTQIAMLGESYKWQRWYFGGADISNPSR
                     PGEPLLYLPLAVLSQVEMNKYGALQSDRNGVQMTARDAETATSFGQLSLPLQIGGPAL
                     IPRATADREPASDWGAPTVSRGFGAGSTEAHALFRLPSSTGPAYIFADGYYARRFFNS
                     LSRAENAGEATLMAALNPNWISGDRLHVALQARGRQNLHAEYFTPEAETMQSGQVSAL
                     AQYNFVSSTAQGALALGYSYRNLAARTSGLSRSATDQLIQAPPLFPEKTHTLFIDASG
                     FKRIPKEFADIEYGINSRFELENRRTEPGSNRLTETLYGTPYAITLYDGPASEANALL
                     RWQPFVRAVRRNARYEYSASGNAHVDWGFTDAGTKLGFVHPAGKIAGRTYLGASNFFV
                     GGAVLHDTLGFTLQEVSFLNRNSLSGTRYNFSDTNGNGVPDNGELGSYSRTGGKYHGA
                     QSGLQAPQKEELNLGFGYEGMQNWRLELNLNGRIYRRLFEVRYAEGTSPTFTPTTAGG
                     SITTYDSTGTGSEYYQLRNAEKDAYYAHAEISLIRLPENTDWIFRGSVGGYYGAGYTP
                     HGLNMFYNDLGAYNESTADPNFRENRFARIDSERGYIGKIQFGRRFAKMLSVTNVLRY
                     RDGEPVAGYRVVTGLAQGPQLLPYEERGGGLTGVGRHTYSLAWDLRLRLDTVIGNAPA
                     WAFLDIYNLLNSRTELAEYPVDGTRFRDPVEQGIARTMRLGFGMNF"
     gene            complement(7843..8793)
                     /locus_tag="PLF85_00605"
     CDS             complement(7843..8793)
                     /locus_tag="PLF85_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18180.1"
                     /translation="MEQGYSQAIADEILNTNFPRATTLAETWLRELPAGAANAEALEA
                     YEIARMFLHRQERFAALAPGIERGKAFEELLGTLMQAHSVTLVRRAAIMLCHAQVADN
                     FARDFAGQKNYQLQLKDLLQLAYSLLMIANYASAREVLTFIIANHPTQATAHYFAAHA
                     ANMTGNETAFFEHYREALYLRPEIVSEYPEFMPGGVFQEMFKIVHEEEYGEGVRERIY
                     ALLLEVNGVYRHRRKLKIDEVRNLEAEYRKLRQEYVAARVHKKAFEPRLLQLLAMLVI
                     HAHQTQNFEKFEQYRGEMVAIDHSIWQTFQQNNLTQGAQS"
     gene            complement(8857..10101)
                     /locus_tag="PLF85_00610"
     CDS             complement(8857..10101)
                     /locus_tag="PLF85_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNE18181.1"
                     /translation="MAEARLQQPHLNTEQRAAAERLWKSSAPALQLIAGAGSGKTTTL
                     VAAICAAVEHGFSSERIAVISFTRKSARELSERLAAPGISPGFCGTIHALAWRLLKAR
                     GNAPRLLVDSASAKSQVIRKSLPGLEHVPDRLLTRPGFLSVADSERVAAAWREHLESQ
                     QLIDFDSMVSRAAELPGIAGLFDAIFIDEFQDTSPDQVAFARALAPAKLFVVGDDWQS
                     IYRFRGADVSVTRDFQRLVPGAERLFLVKNYRSSRQIVALGNATIRQSSVFVRKRLLP
                     TLGPHMKPVVYLTDAAEMTQAWGSFATYLQNASGLPRPLTVLVRTNAQRRLLESSLPA
                     GCEVMTIHKSKGLEFDHVLVFGIAEHSMPHRENDTDEEVRILYVALTRARRFLGFVAW
                     EQNEKRSPFLSFLLKHCKLQYF"
     gene            complement(10105..11013)
                     /locus_tag="PLF85_00615"
     CDS             complement(10105..11013)
                     /locus_tag="PLF85_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HNE18182.1"
                     /translation="MALTPHPNQNPLTIHVLSNARTLYSTRRFFEEGYRRGHKMRVYP
                     PMQMSAYLERNALELYYGDQKIETPHCIIPRLGQKKPEHTLAIIRHYEMMHVKALNAS
                     YPMMRCRDKFVTMQVLAQNRIPVPRSVLIDDPLHVDNAFQKIGEPPYILKIPTGSQGT
                     GVMLADTRKAARSFIETLLDQGLQIIVQEFIAEAQGSDIRAIILGGNLVAAMRRNAAT
                     DDFRSNIHRGATSERVELSIEATRTVKLAARILGLTFAGVDLIESKRGPMILEVNASP
                     GIEGIEGATGVNVAEKVITFLEATAR"
     gene            11130..11810
                     /locus_tag="PLF85_00620"
     CDS             11130..11810
                     /locus_tag="PLF85_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HNE18183.1"
                     /translation="MAKKAEFPAPWELTGSGVIIFFPANKKRVLESGMLSPEDAAAYR
                     GGFGAVMLVYYEKADCGPYDELLYIPGLFEHKGRNYQRITKIFVSSKESVEWGRRNWA
                     IPKELALFDWRQGEREWHIDVKQPDSGASIYSVSLSPRFFSFPVTTAILPWSVLQKQE
                     PKYTEGSPFYLETRPSGKGSGKIAFIDRVEGSREIPDYHDVSHGPRIAVAISPFHMTF
                     PVAKTVRD"
     gene            11803..12729
                     /locus_tag="PLF85_00625"
     CDS             11803..12729
                     /locus_tag="PLF85_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE18184.1"
                     /translation="MTERAYPVLGIDIGATSIKYASLMASGELMFRGSEPVASGSNQQ
                     LVEQLAAVVARPEFAQCTAIGIGSPGPLDLSGGLILASANMPGVQNCALVPELTQRFP
                     GKKFRLDNDANAATLGEWFFGAGKGRSDFAVFTLGTGVGGGCVFQGRLQRGFNGNFFE
                     VGHIPVAAFPAGNSMARRCGCGAIGCLETLASATGISESYYLQTGERCTAAEIASRAG
                     QGDQNAIGAYTVAGQALGLAAATLTQVMNLTAFIFTGGVAAAESLLRPVVKQTWLEHV
                     LPVFHQQLQVTFTRGDEHAGILGAAALFLEEA"
     gene            12726..13208
                     /locus_tag="PLF85_00630"
     CDS             12726..13208
                     /locus_tag="PLF85_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18185.1"
                     /translation="MRTKVLFLLAILNFSVSAVSAQTALSTRLSCEAHFLEAHFAMPQ
                     DDRAKKEHWLMPESMHKSFEKPPLASYAFFRRIESQAWNLEILKPLRMQFVFLPRFEI
                     TFLESRITSLGQAFYRFEYKVNHLQSASFTLDALTSAALFLRHAKTHDEFLHLTCRAT
                     "
     gene            13234..13704
                     /locus_tag="PLF85_00635"
     CDS             13234..13704
                     /locus_tag="PLF85_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18186.1"
                     /translation="MKHNRKTPPRRDDLLQWGVPRLLGAGYGLFSRKAISPRWPEIVI
                     TAAVIKKYRNKSSFWFMVTSATRGVARHARASGELQSVLARLVGSTPRQMQQLAGIYE
                     AMRLEQGTAAAIACVVEIVRITNEKRKHRLAWYRAVPGIQQRPRNPRAHQRNSK"
     gene            13717..14742
                     /locus_tag="PLF85_00640"
     CDS             13717..14742
                     /locus_tag="PLF85_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE18187.1"
                     /translation="MKAIPFQRPSSSEALRVEEWREPTLTTGQVLIEVKAFGLNFADI
                     LARKGQYGDAPRFPFVPGYEVAGIIRQVAEGVQGFSVGDEVLALTLFNGYAELAVADA
                     RAVAKKPAGMGFADAASIPVNFLTAYHALFETGTLRTGSNVLIHAGAGGVGLAAIQLA
                     RNAGCTVYATAGSEKKLALLSDFGVEHPINYQTHDFEREVLRLAGGAKIDVVLDSVGG
                     SYFKKDLNLLRSHGRVVAYGAAAFSERNFLKLPSLLPQVISMLTLSMIDLMMHSKGFY
                     GVNMLRVAQENPDLLQYELQKIMEQFSKKQLKTVVSRQMSWRQIGEAHEALENRSSTG
                     KIVLMVD"
     regulatory      14763..14917
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            14992..15825
                     /locus_tag="PLF85_00645"
     CDS             14992..15825
                     /locus_tag="PLF85_00645"
                     /inference="COORDINATES: protein motif:HMM:NF033170.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13355 family lipoprotein"
                     /protein_id="HNE18188.1"
                     /translation="MKRFLLFTTLVFIAVGCAQIDKDALGESRTVAAANATTHAGILL
                     ATTVVSAPGHTGLGFYDRQKALNGVRGGGTGAQSTDVFSLDNPGFNNDSSTELVLSWG
                     GKRILNGTGIDFIVFENAFYQSGDANARFMDLLIVEVSDDNINYCGFAPDYSAVTETN
                     YSNNPAHWQNFAGVSPVLYNIENNNLTLQQLFEDINNDKVPDQGGGDGFDLALLSDSN
                     TFATGCTMALRDSLRDNGFRYLRLVPASRRINPDTSAAFVSDAASNGPDIDGVAARYS
                     D"
     gene            15839..17824
                     /locus_tag="PLF85_00650"
     CDS             15839..17824
                     /locus_tag="PLF85_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE18189.1"
                     /translation="MRRIFLLLAALLPEIIVAQAECPAGVECAGEAQLRTRRPIRLVS
                     EADKRAAGSYREVPLADAPEAALSERVSDVLQRQSGVQVNRAGAPGTQSLLGVRGASP
                     EQTEYFIEGVPLPRPYAAPLNLETMPLPLFRAVEVYPSFIPAHLAASNIGGALDFRLR
                     RPEGTGYLAQTTASELGGTSIAAARHTATSLHFATIEQSRNSYTYPDNNGTTENTADD
                     RRLRRQNEDYSRLGYTGFGQWETGRWQIGVLADAALTDRGLPGTQGYRLEQVRRREER
                     GATAVRIHYRLSRQQELQSFATASADRMFVSDPAREMLASTGETTLAPQVMSGAGYSL
                     SSGGVSLGIYARARYQDIARNSAFLARRHELQAAASAAYDPGWFRVAAQVAGVGFTDR
                     AAANAFYASAAQEYSGSGVSASALAAFRPLGLLGKNQQDLLELYAQVSSAYRPPSLFE
                     RFGDNIFVTPSENLRSEKALTNSAGLRGSVGCPWGMTCSFRSEFWLTGGRDYILFTQN
                     SSRTLVAVNASAAQIYGIESEAMLSLPGQFLLSLRYTWLEARDYGNIPFYQDKYLPLR
                     PRHHFVLTASYLWQSWRFTASGEFRGAVYRDRYNSYQYYQAARLTADAAVDYLWQNAA
                     RHTLTLAVRNLTDNQTTDMIGYPLPGRYVLVKWMMEF"
     gene            17824..18936
                     /locus_tag="PLF85_00655"
     CDS             17824..18936
                     /locus_tag="PLF85_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18190.1"
                     /translation="MRSQIILKIVFLFFLAQGCTDLPQPARPDAPASGIMLVAGTDFT
                     TGFLSAFEPATGRVYRDIVPLHSDVQLAYQGAPFVLQRLGADSLRRLDQNAGYASVFD
                     QSLGGRNNPQGIAFLPGNQVAVSFYNRNLIDIRSALTGMQTGSIDLSAWADADGFAEI
                     AHLHYQAGYLYAAVQRLNRQATDAVWPPLGSSYLLRIDVSNYSITAYPLTFANPVSRL
                     HYNATRGSLVLAAPGRFAANYSLDGACLEFNLTTFSFVTPPITEAQAQYEIADCDIKA
                     DGSGVFVGYDAQLNSVFGSFDVMTHTVTRVAAQLSSATGGYFAGFQLHSSGKVYLADR
                     NLYQPGLRIFSGTLLTEETAQAVYTGLPPFSLVEVP"
     gene            complement(18954..19433)
                     /locus_tag="PLF85_00660"
     CDS             complement(18954..19433)
                     /locus_tag="PLF85_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNE18191.1"
                     /translation="MKTIDQWLEEYGDSHRNKTNKLIHWICVPTIMFTVLGFFMAIPR
                     GEIFNWVPAEAAFLANWGGVALVLAALFYLRLSLAMFLGMSVIALAMLYGAYQLDKVT
                     SLWLVCLVVFVIAWIFQFIGHRIEGKKPSFFKDVQFLLIGPAWLLGFVFRKIGVKYS"
     gene            complement(19485..21623)
                     /gene="ppk1"
                     /locus_tag="PLF85_00665"
     CDS             complement(19485..21623)
                     /gene="ppk1"
                     /locus_tag="PLF85_00665"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNE18192.1"
                     /translation="MDSSRKTEFAPEHYINRELSWLKFNERVLEEAIDESNPVLERLR
                     FLLITENNLDEFFMVRVAGLKQKLFNDIDAENADGMQVHEVLKQISLNVAGYYERAYS
                     LLDNVLMPLFRERGVQIVSTDDLSAEETGKARRYFDSEIFPILTPLAVDIGHPFPRLA
                     NRSANLAVSLRRKGKDDSPEYFALVQIPSLLPRLFLLSEGTQRRRYIFLEDIIRMFSH
                     RLFKGFDVSEIHSFRLTRDSDLVIEEDEGESLMKVIQLELRRREKGAAVRLEVMRTMP
                     ENTMNRLMQALDLGRDDVYAVSGRLPMYSYKALLDDPLVAAECFKVFTPVSPIKYDSP
                     DDLFSHIRRGDVLLHHPFHSFALVEDLLEAASEDPTVLGIKITLYRTGTRSRIVDALM
                     RAARNGKQVTALVELRARFDEETNIQWAKRMEEEGVHVVYGLIGYKTHCKIALIVRKE
                     ASKIVRYVHMSTGNYNSATARLYTDLSLMTVDKDIAQDATHLFNSITGYSKLPAMQKL
                     ATSPGNLKATVIKLIRAERELAKTGKKGRLIAKMNSLVDPEVIDELYLASQAGVQIDL
                     VVRGICCLRPGVPGISENIRVRSIVGRLLEHSRILVAYNGGDPKIYLMSADWMPRNFI
                     RRVEIAFPIENKSVRETILKNILQRYLDDTESARILQPDGTYRRIREAGKEGMNVQRT
                     FIAESRLGVEEKPPSGKKLHKKAKRPAPGK"
     gene            complement(21663..23132)
                     /locus_tag="PLF85_00670"
     CDS             complement(21663..23132)
                     /locus_tag="PLF85_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNE18193.1"
                     /translation="MQPNIFLEGNFAPISEELTLNTPDNFDIKGTIPRDLHGSLYRNG
                     PNPAQRVAANHHWFLGDGMIHSFHFARGGVAYRNAWARTPTFNFEQRSGKSLFLGPNL
                     ALLANAQLVGFEVFSLLGGLIRHGNADSYTRLISKANTAILPFRDHVYALVESSPPLR
                     LSGASLETEGFENFGSNFNAPFTAHPKVDPQSGYLYAFGYRVFGKPRLEYYVINPSGK
                     LISRTPVETPYAAMIHDFAITRNYALLPVFPAVSSLASIQRGRIAEWQPDKPAVLYLL
                     QRSGEKQTLRTLELPAGYVYHYANAYEDAGAIYLDGFFYDKVPLMGSDEEIREELFAG
                     VNPGAFSRFRIDLQTGKVEKQDLWQAGFAEFPVIDNRVTGEKYEYAYAALRRPECNAT
                     EGIFDSHVSFHIQKGKLRSEITPLPPGHFGGEPVFVPTGKPGQNLGYLLNIIYDSNSD
                     HSYLGIFDAAKPDRRAVCEVHVSHRIPYGFHGAWRSGRN"
     gene            23348..>23757
                     /gene="fadH"
                     /locus_tag="PLF85_00675"
     CDS             23348..>23757
                     /gene="fadH"
                     /locus_tag="PLF85_00675"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804373.1"
                     /note="catalyzes the formation of trans-2- enoyl-CoA from
                     2,4-dienoyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNE18194.1"
                     /translation="MSLQYPNFLSPLDLGFARLRNRSLMGSMHTALEETENGFARLAA
                     FYAERARGEAGIIVTGGIAPNPAGRVFTGAAKMDTEHEADEHRVITKAVHDNGGLIAM
                     QVLHTGRYGYHRDCVGASEIQAPINFVKPKKLSH"
BASE COUNT         5013 a         6514 c         6818 g         5412 t
ORIGIN      
        1 ccgcctgatg cgcttaggtc agctatgggc ttaagcccat agctgaccga ttcgaaatcc
       61 cagggttgtc agaggccccc gagatttctt ctagctacgg gcttaagccc gtagctagaa
      121 attattcggc acttggtgcc tgcaattgca cgtagttctg tagacccatc ttgtcgatca
      181 gacccagctg ggtttcaatg aagtcgacgt gctcttcttc tgattcgaga atttcagcca
      241 atagctcgcg ggtgacgtag tcgttgacgc tctcgcagtg cgcgatcgag cttttgagca
      301 gtggcacggc ttccatttcg agtgccaggt cgcccttcag gatttcttca actgtctcgc
      361 cgatgcgcag gcggccgaga tcctgcaggt tgggcaggcc ttcgagaaac agaatgcgct
      421 cgatgagctt atcggcgtgc ttcatctcgt cgatcgattc tttgtactcg taagctccca
      481 gcttttccat accccagttc ttgagaatgc gggcatgcag gaaatactgg ttaatacctg
      541 tcagttcgtt cttcaggtta gcgttgagca gttcgatgac ctttttatcg cctttcatag
      601 gctttcttcg cacgcggtaa caccacggcg actaaaaagc ggttcataac caaaactcgt
      661 tgcatagacg gaagagagaa aaatcccaca gtgcaccgca ccgaacgcaa agcctggctt
      721 cggcagaata ttgttgaccc gcccccccgc gttttatcag actgccggcc atgtcaaaag
      781 acgccaagga tcacgccctc gaatctgcga cccacgcccc gtggcagcgc gagttcttca
      841 agaacatcga catcttcatg caggcgggcc tgaaagaaga aaaggcgcgc gagctgctga
      901 tcaagtacct gaagctctcg gtggatacac cgattccgcg tgtcatggag acgttcaagg
      961 acgagtccaa gcttgaagag gtcggcgtct tcacgcagcg cgacgagaag ctgcgggatt
     1021 tcatgattga gtttcttacc ccgatgctca agaaattcca tatcgaggga gaagaaaacc
     1081 tcgatgaaat tctgccgcta ttgggaaaat accctgtgac gctgatctct aaccacctga
     1141 gccatttcga caccgcggcg atctatggac tgctgtataa tcacagcaaa gaagcgcgca
     1201 agctggccga ttcgatggtc tttattgccg ggcgcctggt atttcttgcc gacttcaccc
     1261 gcctgggtct ctatacgatt gagagccttc tggtctgctc gaagcgcgac atgacggaca
     1321 accccggtat ggccgacatg atgacgcgca tcaacatgcg ctcgttcagg caggcggccg
     1381 acctgcagaa gcagggcaag gtcatctgta ttttccccga gggtacacgc agccgcacgg
     1441 gccgcctgat tacctttgtc gacacggtct accactatgt catgaacaag gtcatcgtgc
     1501 ctatctcgct ggttggtacg gacaagatct tgccggcgga gagctttctg ttccagcagg
     1561 cgaccggcaa gctcgtgatc ggtaagccga ttctcgtggg caaactgcat ggtgaatctg
     1621 ccaaaacttt ccctgagcac atccagcgcc tcgagattcc cgaagtcgaa aacaagaagc
     1681 agttcgtgat cgactcgctg gcactgttga tcggccagaa cctcgaccgg cacatgcacg
     1741 gtacctaccg caacctgtac cttgatgacg gcaaaaccaa gaagaaaaat atcctgatca
     1801 aggctgcacc gaccccgcat gccgaggtcg gcattatcgg gcacagcccg tatgcaacgg
     1861 cgatggccgc ggtgctggcg aacaagcagg tcaacatacg cgtctatctg cccgatgcgg
     1921 aaaaggccag gacttttaac gaggtcaata ccgacctcga aaacttcccg atcttcaagc
     1981 tgcctcccaa catcgcgttt acctcagacc ccaaagacct gctcaagtgc tcgatttacc
     2041 tgcagggcgt gcgcccgtgg gagctcgaaa agtactacga gcaggtgacc ccggtgctaa
     2101 accaaagttt cgccacgatc attggtatcg tgaaggggtt taccggctca aaatatggcc
     2161 tgatattcga agacctggaa aaaatctaca agatcaatcc ggcgcgtttt gccgtacttt
     2221 cgggggcaaa ttacccggaa cagattctgg agcgcaaacc gggtggtttc gagctcgctg
     2281 cagtgaatac ctctttggtg agcgagctga tcgacttctt cacgactggt tatgtctaca
     2341 cgcggccggc ggtaaatccg tacgatgtgc gcggcctgca gattggcggg gcgctgaaga
     2401 atatctatgc gctgggcgtc ggccttgtcg atggctacta cgaaaaacag ctgggtggta
     2461 acaacgacag tacactgttt cacctggcgc accgcatttc agccgaaatg gccaacatgg
     2521 ttgtcgagat gggtgggcgt aaatcaacgc tggccggtgt cgcgggtatt actgacctga
     2581 tgctcagctg cttcgggcag gatagccatg accggcagta tggccatgac tttgtgttcg
     2641 atacccacga taagaagaag cagctcagtg gagtctacgg catcaaggcg ctgccccggc
     2701 tcgtgcgcct gcgcgaagag aagtacccga ttgcgaaggc aatccatgat gttatagccg
     2761 acggcaagga catcgacgac gccatcgagc gtatcgctga gacctttggc caatcccggg
     2821 cagttcaatt cagggattag atagcatgtt tttgggtgcg cagagcgcac ccaaaaacat
     2881 gctaaaagaa acgacataac tccacgataa ttaaaacgtg gaatacgtag cgttcttgtt
     2941 ccttggcctc ggggtcttat tggttcttgc aggcgtcctg ttgcaggcta acgaaaaacg
     3001 ggcggggggc aggtcaccgc tcgaagacta tgcgctacag tcggcccggc ctgaaacccg
     3061 tgcgaatcca ctgtctgcac tcaagacctc ggctatgcca gcggttgcgg cagctgcccc
     3121 ggttgtgcag ccggcgccgg caatgagctc ggctattccc gggccattcc agtttgtgca
     3181 gcatgaaccg gctgcacccg tgccggtgca agcgccggcg gtggcaccgg ttgcggtctc
     3241 tgttgggcag gcaaatccgc ggttgttcga aaagtttgcc tatctctacc tcgattcgag
     3301 cgcgcgcaac gtttatgacg gagcgggttc agttgctgcc tttgatctca gcgaggtcca
     3361 gggtatccgc aggtttggtc gtggcctctt ttcgtatgat ggttttggct tctattttga
     3421 ccatgccagt ggccaggagc gttttcccct cacaaacctc aagaccattg gtttttatcc
     3481 gaactgtgtt gcgttgacgt ttcgcacctc ccagccggtt gctttactct tcatggaaga
     3541 aacagaaacc atccgcaaag tcctggagac attccgcgcg gaagccgctg gaaatgtctc
     3601 cggctaatcc gcgccccgtg cgcgggtaat gctctgggaa ttgcagggct atcaggacat
     3661 gcgcgcatac gacttcagca cacacaataa aaccgttaag cgggataaaa gccgttattt
     3721 tagttatgcc tttattttcc tgctgttgct ggcaggatct gcctttgtct attatgtgat
     3781 cctgaattct ccgcgaatcc tgtactattt cagggaaaac aagtactccg aaattgagcg
     3841 ggcccatgcc gcggcaatta aggcgattca ggttctgccc gcagaacagg cagcaagtgc
     3901 taaaaatggt gccctggcgc tggccgaagt gcaggatttc attgagctgg cacaggccct
     3961 gcagaaggat caccgcgagg accctatcct gacatacctc gaagctacgg tactcgatga
     4021 gatcattcgc cgccaggttg ccgaaaagcc cgaggcgctg ctggcgcttt tattccgcga
     4081 attcattggg cgaccggcgt tcccggcaga gtttgaccgt gagctttggc agcgcgcgct
     4141 actggcaggc cgcaaggcgc gttcgctggg tttacctgaa gtcttgagcg cacggctggc
     4201 cgaggcggag ctcgatgtgt acctgctggg tggcaggcca tggtgggaaa gcgcgcagga
     4261 gattgccgcc agcaatgcca cagcgaaaaa gctgccttca tggcacctga tgcaggccgg
     4321 tatggcgcgc gaaactcctg attttgcgct ggtccagaca gcctatggtc caaccactgc
     4381 ggcatttgcc aagggcgttt attatatccg gtcgggaaat tctccgctgg gtatttccaa
     4441 tttccgggat ctcagcaaga gccaggctga tgcatttgcc cgcgaccatg cgctctacac
     4501 gctgggtttt ctgagtgccc gggaaaaacg catacgtgac cagctgtcct attacaaaca
     4561 gattcgcttt cccgaatttg ctccacgtta tccttttttt gtacctgagc tgaattacct
     4621 gctgcgcttc ctggggcagc aaggcgaggc cgaccagatg atgaaagcct gggaagagct
     4681 caaagcacag gaaaaatcgc agtagcaaat cagttgacct gcgccctgaa acctgcgctt
     4741 caggcccatg gatatcctcg cgaaccctta cctcaatatt gtgtttaatg cccttatcag
     4801 cgggggcgcc ttcctgtgtt tcgtgctggg tatcgcgatg cttttgctga agtacccgag
     4861 gcgttctgtc agctacctga tcttgctgag ttttgtcatg ggtatcacgc agctctatgc
     4921 ctggtttaac ctcaacacgc tgttcttcag accgcagtgg ctgaattaca tctttgtcgg
     4981 tgcaaatttc ctgattggcc cgggcgtgta ctatttctac aaatcgaccg gtgatgactc
     5041 attcgtggcc gaccgccgga cgcagctggc gtttctaccg ggcatgttct ttagcctgct
     5101 gattcccttt attaaccttg tcgctcctga agtcatgccg actgacccaa gacagttctt
     5161 ctatacaggc cgacccagct ttatcgatgc catcttttcg ctcgcattgc tgcacaatgc
     5221 aggctactat atcaagttgt tcctggtcac tcggccgctg atcgtggcaa acaggaatat
     5281 gaaagagaca ggtgcactca ctgccttcct catgtttttc ggtatcatta cgctgatcaa
     5341 cctctacggc atcattgcgt acctgacacg cgacatccgg catttttatg ccgattcatg
     5401 catcattacg ctgctcatcg tgtcattcat ggtgtttgcg ctgcgctacc cgcagtattt
     5461 tctcagggtc aggccggagt aatcgctaga aattcatacc aaagcccagc cgcatagtcc
     5521 gggcgatccc ctgttccacc ggatcgcgaa aacgcgtccc atcaacgggg tattcggcga
     5581 gttcggtacg cgagttcagc aggttgtaga tatcgagaaa ggcccatgcg ggagcattgc
     5641 cgatcacggt atccagcctt aggcgcaggt cccacgccag cgaatacgtg tgccggccca
     5701 caccggtgag tcctccgcca cgttcttcgt acggcaggag ctgcggaccc tgtgcaaggc
     5761 cggtcactac ccggtaacct gccacgggtt caccgtcccg gtagcgtagt acattcgtta
     5821 cgctcagcat cttagcaaag cggcggccga actgtatttt gccgatgtag ccccgctcac
     5881 tgtcgatgcg ggcaaaccgg ttttcgcgga aattcggatc tgcagtgctc tcattatagg
     5941 cgcccaggtc gttatagaac atgttgaggc catgcggcgt gtagcccgca ccgtagtaac
     6001 ctcccaccga accgcggaaa atccagtcag tattttccgg tagcctgatc aggctgattt
     6061 cggcgtgcgc ataataggcg tccttttccg cgttgcgcag ctggtagtac tcactgccgg
     6121 tacctgtact gtcgtaagtg gtgatgctgc cgcctgcagt cgtcggcgta aaggtcggtg
     6181 atgtcccctc ggcataacgg acctcaaaca ggcggcggta gattcgtccg ttcaggttga
     6241 gctcaagccg ccagttctgc ataccttcat agccgaagcc caggttgagt tcttcttttt
     6301 gcggtgcctg caggcctgat tgcgccccat ggtatttgcc gcccgtacgg ctataactgc
     6361 cgagttcgcc gttgtcgggt acaccgttgc cattggtgtc gctgaaattg tagcgcgttc
     6421 cgctcaaaga attcctgttc aggaacgaga cttcctgcaa tgtgaacccg agcgtgtcgt
     6481 gcaggactgc gccgccgaca aagaagttgg aagcgcccag gtaggtacga ccggcgatct
     6541 taccggcagg atgcacaaaa ccgagtttgg tgcctgcatc ggtgaaaccc cagtcgacgt
     6601 gggcattgcc agaggccgag tattcatagc gtgcgtttct cctgaccgcc cgaacaaagg
     6661 gctgccagcg cagcagagcg ttggcctcgc tggcgggccc atcatacagg gtgattgcat
     6721 agggagtgcc gtagagtgtt tcggtcagcc ggtttgagcc gggttcagtt cttcggtttt
     6781 ccagttcaaa gcgcgaattg attccgtatt caatatcagc aaactctttg ggaatgcgct
     6841 tgaagccaga agcatcgata aacagcgtat gcgtcttttc agggaacagc ggcggggcct
     6901 gaatcaactg gtccgtagcc gaacggctga ggcccgaggt cctcgcggcc aggttgcggt
     6961 aagaataccc gagtgccagt gcaccctgtg ccgtgcttga aacaaagttg tactgcgcca
     7021 gtgcagaaac ctggccgctc tgcatggttt cagcttcggg ggtaaaatac tcggcgtgca
     7081 ggttttgcct gccgcgggcc tgcagtgcta catgcagacg gtcaccggat atccagttgg
     7141 ggttgagcgc cgccatcaat gtcgcttcgc cagcattctc ggcacgcgag agggaattga
     7201 agaagcgccg ggcgtagtag ccgtcagcga aaatatacgc cggcccggtg gaagaaggca
     7261 gccggaacag tgcatgcgct tctgttgagc cggcgccaaa gccgcgcgac acagtaggag
     7321 ctccccagtc gctggcaggc tcgcgatcgg ccgtggcgcg cggtatcagt gcagggccgc
     7381 ctatttgcag gggcaggctt agctggccga acgacgttgc cgtctcggca tcgcgcgccg
     7441 tcatctgcac gccattacgg tcagactgca gcgctccgta cttgttcatt tctacctgtg
     7501 acaggactgc gagcggcaga tacagcaggg gttcacccgg ccttgaggga ttcgagatat
     7561 cagccccgcc gaaataccag cgttgccatt tgtaggactc gccgagcatg gcaatctgcg
     7621 tgttttctgc gcgcgcaaaa ccgccggtat cggtgtttgc catgactgca ggctggtaga
     7681 gaaattcaag caagcctgcg acgtgaccgc cgctgggtaa ttcgccgata tcggcctgcg
     7741 tgatacgcca gttggcgtgg ggcaggcggc ggtccgctgg ggcaagctct gcgccagcga
     7801 gcattgtata cggtagtaag gaaagcaaaa gggataattg cttcatgatt gtgcgccctg
     7861 tgtcaggttg ttctgttgga acgtttgcca gatggaatga tcgatcgcga ccatttcacc
     7921 gcggtattgc tcaaactttt caaagttctg tgtctgatgt gcatggatga ccagcattgc
     7981 cagcagctgc agcagtcgcg gctcaaatgc ctttttgtgc acccgtgctg ccacgtactc
     8041 ctgccgtaac ttgcggtatt cggcttcgag gttgcgtacc tcgtcgatct tgagtttcct
     8101 gcgatggcgg taaaccccgt tcacctcgag caacagggcg tagatgcgct cgcgcacacc
     8161 ctcgccgtac tcctcttcgt gcacaatttt gaacatctcc tggaacacgc cgccgggcat
     8221 gaattcgggg tattcagaga cgatttctgg tcgcaggtat aacgcttcgc gataatgctc
     8281 aaagaaggcc gtttcattgc ccgtcatgtt ggccgcatgc gcagcgaagt agtgggctgt
     8341 ggcctgggtg ggatggtttg cgatgatgaa ggtgaggacc tctcgcgcag aagcataatt
     8401 ggcgatcatc agcagcgagt aggcgagctg taaaagatct ttcagctgta gctggtaatt
     8461 tttctggccg gcaaagtcgc gggcaaagtt gtctgcaacc tgtgcatgac agagcatgat
     8521 cgccgcgcga cgcaccagcg taacagaatg tgcctgcatg agtgtaccga gcagctcttc
     8581 aaatgctttg ccacgctcaa tgccgggtgc aagggccgca aacctctcct gccggtgcag
     8641 gaacatgcgg gcaatctcgt aggcttccag agcttccgca ttggccgcgc cggccggtaa
     8701 ttccctcagc caggtttcgg ccagcgtggt cgcccgcgga aaattcgtgt tcagaatttc
     8761 gtctgcgatt gcctgggaat acccctgctc catgagtggg gcactgtgtg tattaaagaa
     8821 gcgacgtaaa ctgtattggc tggccggcgc tgacggtcag aagtactgta gcttgcagtg
     8881 tttgagcaga aatgacaaaa acggggagcg tttttcgttc tgctcccagg cgacgaatcc
     8941 gaggaaacgc cgcgcccggg tcagggcaac gtagagtatc cgaacctctt cgtcggtgtc
     9001 gttttcccgg tgcggcatac tgtgttcagc aatcccaaac acgagcacat ggtcaaactc
     9061 gaggcccttg ctcttgtgga tcgtcatcac ctcgcagcca gctggcagtg aagattcgag
     9121 cagccggcgc tgcgcgttgg tgcgtaccag cacggtcagc gggcgtggca accctgaggc
     9181 gttttgcagg taagtggcga aactccccca tgcctgtgtc atttcagctg catcggtgag
     9241 gtagacgacc ggcttcatgt gagggcccag tgtcggcagc agtcgcttgc gcacaaaaac
     9301 tgaagactgc cggatggtcg cattgcccag cgcaacgatc tggcgggagc tgcggtagtt
     9361 cttcaccagg aacaggcgct cagccccggg aaccagtcgc tggaaatcac gcgtcaccga
     9421 aacatcggcc ccgcggaagc ggtagatgct ctgccagtcg tcaccgacga cgaaaagttt
     9481 agccggcgca agtgcgcggg cgaaggcgac ctggtcgggc gatgtgtcct ggaattcatc
     9541 gatgaagatg gcatcgaaga gcccggcaat gcccggcaat tctgctgccc tcgacaccat
     9601 cgaatcaaag tcaataagtt gctgcgactc gagatgctca cgccaggctg cagcgacgcg
     9661 ttccgaatct gcaaccgaga gaaagccggg ccgcgttagc aggcggtcgg gcacatgttc
     9721 gagtcccggt aaagacttgc ggattacctg tgattttgcg ctggcagaat ctaccagcaa
     9781 gcggggcgcg ttgccccggg ctttcagcaa ccgccaggca agcgcatgga tggtgccgca
     9841 gaaacccgga ctgatacctg gagcagcaag acgctccgat aattcccgcg ccgatttgcg
     9901 ggtgaaagag atcaccgcaa tgcgttcact cgaaaaccca tgttctactg cagcgcagat
     9961 cgcggccacc agggttgttg tcttgccgct gcccgcgccg gcgatcagct gcagcgctgg
    10021 cgcagaagat ttccatagcc gttcggctgc agcgcgctgt tctgtgttga gatgcggttg
    10081 ttgaagcctg gcttctgcca cggatcagcg tgcagtagcc tccaggaatg taatcacttt
    10141 ttctgccacg ttaacaccag tggccccttc aatgccttcg atgccgggtg aggcattcac
    10201 ttcgaggatc atcggtccgc gctttgattc gatgaggtca acgccggcaa aggttaagcc
    10261 caggatgcgc gctgcgagtt tgacggtgcg tgtggcttcg atcgagagtt cgacacgctc
    10321 tgaagttgca ccgcggtgta tgttcgatcg gaagtcatcg gtggcagcgt tccgccgcat
    10381 ggctgcaacc aggttgccgc cgaggataat ggcgcggata tcagagccct gtgcctcggc
    10441 gatgaactcc tgcacgataa tctgcagacc ctggtcgagt aatgtctcga tgaagctgcg
    10501 ggccgccttg cgcgtatcag ccagcatgac tcctgtaccc tgcgaccccg tggggatctt
    10561 caggatgtac ggtggttcgc caattttctg gaacgcgttg tcgacatgca gcgggtcgtc
    10621 gatcagcacg ctgcggggta ccgggatacg gttctgtgcg agcacctgca tggtgacgaa
    10681 cttgtcgcgg cagcgcatca tcgggtagct ggcattcaat gctttgacgt gcatcatctc
    10741 ataatgcctg atgatcgcca gggtgtgttc gggttttttc tgccccagcc tcggtatgat
    10801 acaatgagga gtttcgattt tctggtcgcc gtagtacagt tcgagcgcat tccgctcgag
    10861 gtaagccgac atctgcatcg gtggatacac gcgcatcttg tgcccgcgcc ggtagccctc
    10921 ttcaaaaaag cgccgtgtcg aatacagtgt gcgcgcattt gatagcacat gaatcgtcag
    10981 gggattctgg ttgggatgtg gggtgagggc cacgtcaggc agatccggga gaaaattcac
    11041 cctgcaaaga tatttcagcg cctgaacggc tggctgttag tgcagcgtac atttgctcga
    11101 cgacctgtgt tgctgttgcg tctgttcgca tggcaaaaaa agcggaattc cccgcaccgt
    11161 gggagctcac gggcagcggt gtaatcatct tcttcccggc gaataagaag cgcgtgctcg
    11221 aatcgggaat gctgagccct gaagatgctg cagcgtaccg cggcggcttt ggcgcggtga
    11281 tgctggtgta ttatgaaaaa gcggattgcg ggccttatga tgaattgctc tatattccgg
    11341 gcctgttcga acacaagggc cggaattacc agcgtatcac gaaaatcttt gtttcgtcaa
    11401 aagaatctgt cgaatggggc cggcgcaatt gggcgatacc gaaagagctg gcgctgtttg
    11461 actggcgcca gggtgaacgc gaatggcata ttgacgtgaa acaacccgac agtggcgcaa
    11521 gtatctactc ggtctctctt tcgccgcggt tcttttcgtt tcctgtaact accgcgatcc
    11581 tgccatggtc ggtgctgcaa aagcaggaac caaaatacac tgaaggtagc ccgttctacc
    11641 tggagacacg gccctcgggt aagggcagcg gcaaaattgc ctttatcgac cgtgtcgaag
    11701 gttcgcgtga aatccccgat taccatgacg tctcgcatgg gccgcgtatc gccgtcgcta
    11761 tctcgccttt ccacatgact tttcctgtgg ccaaaaccgt tcgtgactga gcgggcttac
    11821 ccggtactgg gcatcgatat cggtgccacc agcatcaagt atgcctccct catggcgtct
    11881 ggtgagttga tgttccgcgg cagcgagccc gtggcatcgg gcagcaacca gcagctggtc
    11941 gaacagctgg cggcggttgt cgcacgcccg gaatttgccc agtgcacagc gatcggtatc
    12001 ggctctcccg gtccactcga tctgtcaggg ggattgatcc tggcttctgc caatatgccg
    12061 ggggtacaga attgcgcgct ggtgcccgaa ctcactcagc gttttcccgg taagaaattt
    12121 cgcctcgaca atgatgcgaa tgcagcgacg ctcggcgagt ggttctttgg cgcgggtaaa
    12181 ggccgctctg actttgcagt gtttaccctg ggtaccggcg tcggtggtgg ctgcgtattc
    12241 cagggcaggc tgcagcgcgg cttcaacggt aactttttcg aggttggaca cattcccgtg
    12301 gcagcatttc cagctggcaa cagcatggcg cgccggtgcg gttgtggcgc tatcggttgc
    12361 ctcgagactc tcgcctctgc caccggaatc agcgagagtt attacctgca gaccggcgag
    12421 cgctgtactg cggcagagat tgcatcccgg gcagggcaag gggatcaaaa tgcgatcggt
    12481 gcttatacgg tagcagggca ggcgctgggc ctcgccgcgg cgacgctcac ccaggtaatg
    12541 aacctgactg cattcatctt cacaggcggt gtggcagcgg ccgaaagcct gctgcgtccc
    12601 gtggtaaagc aaacctggct tgaacacgtg ttaccggttt ttcaccagca gctgcaggta
    12661 acctttacgc ggggcgatga acatgcaggc attttgggcg ctgcagcgct ttttctcgag
    12721 gaagcatgag aacgaaagta ctttttttgc tggcgatcct gaacttttct gtatccgctg
    12781 tctctgcgca gacagcgctg agcacacggt tatcctgcga ggcgcatttt cttgaagcac
    12841 actttgccat gccccaggac gaccgcgcaa aaaaggagca ctggctgatg ccggaaagta
    12901 tgcacaaatc ttttgaaaag cccccgctcg cttcttatgc ctttttccgc cgtatcgaat
    12961 ctcaggcgtg gaacctggag attctgaaac ccttacggat gcagttcgtt tttctgcccc
    13021 ggttcgagat aacatttctt gaatctcgca tcacgagcct gggacaggcg ttctaccgtt
    13081 ttgagtacaa ggtgaatcac ctgcagagcg ccagttttac gctcgatgcc ctcaccagcg
    13141 cggcattatt cctgcggcat gccaaaacgc acgacgagtt tttgcatttg acatgccgcg
    13201 caacttaact cgttcctacg ccgcggtaag cccatgaagc acaacaggaa aactccaccg
    13261 cgtcgtgatg acctgctcca gtggggagtg ccccgtttac tgggggctgg ctacggcctg
    13321 ttttcgcgca aagccatttc accgcgctgg ccggagattg ttatcactgc agcagttatc
    13381 aaaaaatacc gcaacaagag ttcgttctgg ttcatggtca ccagcgcgac gaggggagtc
    13441 gcgcgccacg cccgggcaag cggtgaactg cagtcagtgc ttgcgcgcct ggttggttct
    13501 acgccacggc agatgcagca gcttgcaggt atctacgaag cgatgcgact cgaacagggt
    13561 actgctgcgg ccatagcctg cgttgtcgaa attgtccgca tcaccaatga gaagcgcaaa
    13621 caccggctcg cctggtaccg ggcagtgccc ggcattcagc aaaggccacg aaacccgaga
    13681 gcgcatcaaa gaaacagtaa atgagaggtt aacagcatga aagcaattcc cttccaacgg
    13741 ccatcttcgt cagaagcact cagggtagaa gagtggcgcg aaccaacgct tacaaccggc
    13801 caggtactca tcgaggtaaa ggcgtttggt ctgaactttg cggatattct cgcgcgtaag
    13861 ggacagtacg gcgacgctcc cagatttcct tttgtgccgg gttacgaagt tgctggcatc
    13921 attcgccagg ttgcagaggg ggtccagggc ttcagcgtcg gtgacgaagt actcgcgctg
    13981 acgctcttca atggttatgc cgaacttgcg gttgctgatg cgcgtgcggt cgcgaagaaa
    14041 ccagcgggta tggggtttgc cgacgcggca tctattccgg tgaatttcct gacggcctac
    14101 cacgcgctgt ttgaaacggg aaccctgcgt accggctcga atgtcctgat ccatgcgggc
    14161 gcaggcggag tggggttggc ggcaatccag ctcgcacgta atgccggctg cactgtctat
    14221 gccacagcag gttccgaaaa gaagctggcg ctgctttcgg atttcggggt tgagcacccg
    14281 atcaattacc agacgcacga ttttgagcgc gaggtgctgc gcctggctgg cggagcaaaa
    14341 atcgatgtcg tgctcgattc ggtgggtggc agctatttca agaaagatct gaacctgctg
    14401 cgctcccacg gacgcgtagt ggcctacggc gcggccgcgt tttctgagcg caatttcctg
    14461 aagctgccat ccttgctccc gcaggtgatc agtatgctga cactctcgat gattgatctc
    14521 atgatgcaca gcaagggttt ttacggcgtc aacatgctgc gggtcgcgca ggaaaatcct
    14581 gatcttctgc agtatgaact gcagaagatc atggagcagt tcagtaagaa gcaactgaag
    14641 actgtagtat cgcggcaaat gtcctggcgg cagatcggcg aagcgcacga agcgctggaa
    14701 aaccggtcga gcacgggcaa gattgtgctc atggtagatt agttgacggg atgtcaaatt
    14761 ggcgcaacat tacgtacgcg acgcgtacga tacgctgcgc aaaagaggaa gtccggtgag
    14821 aatccggcgc tgacccgcaa cagtatgtgc ataatcattc tggctccgcc agaatgatta
    14881 tgcaccagcc tggaactctt gctgattcac cccgaggaca cgggtgtttt ggcgaaacgt
    14941 tgccggaaaa ttttttattc cagtaacaag cgggctcctt tgggggctga cgtgaagaga
    15001 tttctattat ttaccactct ggtttttatt gcggttggtt gcgcgcagat cgacaaggat
    15061 gcgctcggtg aaagccgtac tgtggcagct gcaaatgcca caacgcatgc cggcatactg
    15121 ctggccacga cagtagtatc ggctccggga cataccggcc tgggttttta tgaccggcag
    15181 aaggcgctca acggcgtgcg cggcggtggc actggcgcac agagtaccga cgtcttctcg
    15241 ctcgataatc ccggtttcaa caacgatagc tccaccgaac tggtactcag ctggggcggg
    15301 aaaagaattt tgaacggtac cggtatagat tttatcgtct ttgaaaatgc tttttaccag
    15361 agcggcgatg cgaatgcgcg tttcatggat ttgctgatcg tcgaggtgag cgatgacaac
    15421 atcaactact gtggttttgc gcctgactac agtgctgtaa ccgaaacaaa ttattcaaac
    15481 aatcccgcgc actggcagaa ttttgccggg gtgtcacccg tactctacaa tatcgaaaac
    15541 aataatctca cgctgcaaca gctgttcgaa gatattaaca atgataaggt tcctgaccag
    15601 ggcggcggcg acggttttga tttggctttg ctttctgaca gcaatacctt cgctacgggg
    15661 tgcacgatgg ctctgcgcga ttcgctgcgc gacaatggtt ttcgctacct gcggttggtg
    15721 cctgctagcc ggcggattaa ccccgatact tcggcggctt tcgtgagcga tgcggcatcc
    15781 aatggtcctg acatagacgg tgttgcggcg cgttattctg actgaagacg gcgctcacgt
    15841 gcggcgaatc tttcttctac tggccgctct tctgccggaa attattgtag ctcaggctga
    15901 atgtcctgca ggggttgagt gcgcaggtga agcacaattg cgcacgcgcc gccccatacg
    15961 actcgtcagc gaagcagata agcgggcagc tggttcatac cgcgaggtgc cgctggccga
    16021 tgcacccgaa gccgcgctct ccgaaagggt ttccgatgtt ttgcagcggc agagcggcgt
    16081 acaggtcaac cgcgccgggg cgcccggcac gcagagcctg ctcggtgtgc gtggggcttc
    16141 gcccgagcag accgaatatt ttatcgaggg tgtgccgctg cctcgaccct atgccgctcc
    16201 gctcaacctg gagacgatgc cgttacccct ctttcgcgcc gtcgaggtat acccgtcttt
    16261 tattcccgcg cacctggcag catcaaatat tggcggagcg ctggacttta gactcaggcg
    16321 tccggaaggt actggctatt tggctcagac taccgcttct gagctcgggg gaacaagcat
    16381 agcggcagcg cggcacactg ctacctcgct ccacttcgcg acaattgaac aaagtcgcaa
    16441 ttcttacacc tacccggaca ataacggtac gaccgaaaat acagcagacg atcgcaggtt
    16501 gcgccggcag aatgaggact attcgcgcct gggttataca ggctttggcc agtgggagac
    16561 cggtcgctgg cagattggcg tacttgccga tgcggcgctg accgaccgcg gcttgccggg
    16621 tacgcagggt taccggcttg aacaagtgcg ccggagagaa gagcggggcg caacggcggt
    16681 gcgcatccat taccgcctta gccggcagca ggaactacaa tccttcgcga cggcttcagc
    16741 tgaccgtatg ttcgtgagcg accctgcgcg agagatgctc gccagcacgg gagaaacaac
    16801 cctggcgccg caagtaatgt ccggagcggg ttacagtctt tcatccggtg gcgtgagcct
    16861 cggcatctat gcacgcgcac gctaccagga tatcgcccgg aattctgctt ttctcgcgcg
    16921 gcggcatgaa ctgcaggccg ctgcaagcgc ggcctatgac ccgggatggt tcagggtggc
    16981 cgcacaggtg gcaggtgttg gttttaccga ccgggcagcg gccaatgcct tttatgcgtc
    17041 tgctgcacag gagtacagcg gcagcggtgt gagcgcttct gcgctcgcgg cgttcagacc
    17101 gctggggctg ctgggaaaaa accagcagga cctgctcgag ctctatgcac aggtaagctc
    17161 cgcctatcgg ccaccttcgc tgtttgaacg tttcggcgac aatatattcg tgacgccttc
    17221 ggaaaacctg cgcagtgaaa aggcgctgac caacagtgcg ggcctgcgcg gctctgtcgg
    17281 ctgcccctgg ggaatgacct gttcgtttcg cagtgaattc tggctcacgg gcggcaggga
    17341 ttacatcctg ttcacgcaga attcatcgcg cacgctggtc gcggtcaatg cttctgcggc
    17401 ccagatctat ggcatagaaa gcgaagccat gctgagcctg ccgggacagt ttttactttc
    17461 gttgcgttat acctggcttg aggcgcgtga ctacggcaat ataccgtttt accaggacaa
    17521 gtatttgccg ctgcgcccac gccaccattt cgttctcacg gcatcttacc tttggcagag
    17581 ctggcgtttc accgcctcgg gggaattccg tggtgctgtt taccgcgacc ggtacaatag
    17641 ttaccagtac taccaggcgg cgcggctcac ggccgatgcg gcagtcgact acctgtggca
    17701 gaatgccgct cgacatacac tgacgctggc agtgcgcaac ctcaccgaca accagacgac
    17761 ggacatgatt ggctatccct tgccgggtcg ctacgtgctg gtgaagtgga tgatggagtt
    17821 ctgatgcgct cacagatcat tttaaagatc gtgtttctgt ttttcctggc gcagggttgt
    17881 actgacctac cacaaccggc caggccggat gctcccgcat cgggcatcat gctcgttgcc
    17941 ggaaccgatt ttaccacggg cttcctgagc gcatttgaac ctgcaaccgg cagagtctac
    18001 cgcgacattg ttccactgca cagcgatgtt cagctggctt accagggtgc gccgttcgtg
    18061 ctgcagcggc tcggtgcaga ctcgctgcgg cgtctcgacc agaatgcggg ttatgcctcc
    18121 gtatttgacc aatcgctcgg cggccggaac aatccgcagg gaatcgcctt cctgccagga
    18181 aaccaggtcg ctgtctcttt ctataatcgc aatttgattg atatccgctc agcactgaca
    18241 ggtatgcaga ctggcagtat agatttgtca gcctgggccg atgccgatgg cttcgctgaa
    18301 attgcacacc tgcattacca ggcaggctat ctttacgctg cggtgcaacg actcaaccgg
    18361 caggcaaccg atgctgtctg gccgccttta ggcagcagtt acctgttgcg gattgatgtt
    18421 tccaactaca gcatcaccgc ttacccgctg actttcgcga acccggtttc gcgcctgcac
    18481 tataacgcta cgcggggttc gcttgtcctg gcggcacccg gcaggtttgc cgcaaactac
    18541 agtctcgacg gtgcctgcct ggaattcaat ctcacaacct tcagctttgt gactccgccc
    18601 atcaccgagg cacaggccca atacgaaatt gctgactgcg atataaaggc ggatggtagc
    18661 ggcgtgttcg tgggctacga tgcgcagctg aacagtgttt ttggcagttt tgatgtcatg
    18721 acccacaccg taacgcgagt cgcggcgcag ctgtcgtcgg caacgggagg ttattttgcg
    18781 gggtttcagc tgcattcaag tggcaaggtt tacctcgccg accgcaacct ctaccagccg
    18841 ggattgcgta tttttagtgg aactctgctt acagaagaga cggcgcaagc cgtctataca
    18901 ggattgccgc cgttttcgct ggtcgaggtt ccctgacccc gaccacgatg tcgtcaggaa
    18961 tacttaacgc cgattttgcg gaagacaaag ccgagcagcc atgctggccc gatgagcagg
    19021 aattgcacgt ctttaaaaaa agagggtttt tttccttcga tgcggtgccc gataaactgg
    19081 aagatccagg cgataacgaa gacgaccagg caaaccagcc agagactggt cactttgtcg
    19141 agttggtagg cgccgtagag catagccagc gcgatcaccg acataccgag aaacatggcg
    19201 agcgagaggc gcaggtagaa gagcgcggcg aggaccagtg cgactccgcc ccagttggca
    19261 agaaaggccg cttcagccgg aacccagtta aagatttcgc cgcgcggaat ggccatgaaa
    19321 aatcccaata cggtgaacat aatcgtcggc acgcagatcc agtggatcag cttattggtc
    19381 ttattgcggt ggctgtcgcc gtattcttcg agccattggt cgatggtttt catggcgcaa
    19441 catcgacgag caacggggcg tcgtcaattc aaaataccag gagtttattt cccgggtgcc
    19501 gggcgtttcg ccttcttgtg gagttttttc ccggaaggag gcttttcttc gactcccaac
    19561 cggctctcgg cgataaacgt acgctgtacg ttcatgcctt ctttacccgc ttcgcggata
    19621 cgccggtagg tgccatcggg ttgcagtata cgcgctgatt cagtgtcgtc gagataacgc
    19681 tgcagtatat tcttgaggat tgtttccctg acagacttgt tctctatcgg aaacgcgatt
    19741 tctaccctgc gaatgaaatt gcggggcatc cagtctgcac tcatcaggta aatctttggg
    19801 tccccgccgt tatacgccac gagtatacgg ctgtgttcga gtagcctgcc gacgatactg
    19861 cgcacgcgga tattctccga gatgccaggt acacccggcc gcaggcaaca gatgccgcgc
    19921 accacaaggt caatctgtac cccggcctgc gatgcgaggt aaagttcgtc gatgacttca
    19981 ggatcaacaa gtgaattcat cttcgcgatc aggcgaccct ttttaccagt cttggcgagc
    20041 tctcgttctg cccttatcag tttgatgact gttgccttga ggttaccggg tgatgtcgca
    20101 agtttctgca ttgccggcaa tttggaataa cccgtgatag aattaaaaag atgcgtcgca
    20161 tcctgagcga tatccttgtc gacggtcatg aggctaaggt cggtatagag gcgcgcggtg
    20221 gcggagttgt agttgcccgt tgacatgtgt acgtagcgga caatcttgct cgcctctttg
    20281 cggactatca gcgcaatctt gcagtgtgtc ttgtagccaa tcaggccata gaccacatgc
    20341 actccttctt cttccatgcg cttcgcccac tggatgtttg tctcttcatc gaagcgcgcg
    20401 cgcaattcga cgagcgccgt gacctgctta ccgttgcgcg cagcgcgcat gagcgcatcg
    20461 acgatgcgtg aacgtgtacc tgtgcggtag agggtaattt tgatgcctag aacagtcgga
    20521 tcttcggagg ctgcctcgag caaatcttcg acgagggcaa acgaatgaaa ggggtggtgc
    20581 agcagcacat caccgcggcg gatgtgacta aagagatcat cggggctgtc gtacttgatc
    20641 ggtgagacgg gggtgaacac cttgaaacat tcagccgcaa cgagggggtc atcgagcagc
    20701 gccttgtacg aatacatggg cagccggcct gaaaccgcat agacgtcgtc ccgcccaaga
    20761 tcaagtgcct gcatcagccg gttcatggta ttttcgggca tcgtgcgcat gacttcgagc
    20821 ctgaccgctg ctcccttttc acggcggcgc aattccagct ggatgacctt catcaggctt
    20881 tcgccttcgt cttcttcaat cacaaggtca gaatcgcgcg tcagccggaa cgagtggatt
    20941 tctgaaacgt caaaaccctt gaacagtcgg tgcgaaaaca tcctgatgat atcttctaaa
    21001 aagatgtagc gccgccgctg tgttccctcg gacagcagga acagccgcgg cagcagcgac
    21061 ggtatctgaa ccagcgcaaa atattcgggc gagtcatctt tgcccttgcg cctgagcgag
    21121 acagcaaggt ttgcgctccg gttcgcaaga cgtggaaacg gatgcccgat atctacagcc
    21181 agcggggtca ggatcgggaa tatttcgctg tcgaagtagc ggcgggcctt gccggtttct
    21241 tctgccgaaa gatcgtcggt cgaaacgatt tgtaccccgc gttcgcgaaa gagtggcatg
    21301 agcacattgt cgagcaggct gtatgcccgc tcgtagtaac cagcaacatt gaggctgatt
    21361 tgcttaagga cttcatgtac ctgcatgccg tcggcattct cggcatcgat gtcgttaaaa
    21421 agcttctgct ttaaacccgc aacgcgcacc atgaaaaatt catcgaggtt gttttctgtg
    21481 atcagtaaaa aacgcaggcg ctctaacacg gggttggact cgtcgatcgc ttcttcgagc
    21541 acgcgttcat tgaacttcag ccacgataac tcgcggttga tgtagtgttc cggggcaaat
    21601 tctgttttac gtgaactatc cattcctttt accttatgcc ccgtaatcga tccttattct
    21661 tcttaattcc tgccggagcg ccatgcaccg tgaaaaccat agggtatacg gtgcgaaaca
    21721 tgtacttcgc aaacagcgcg ccggtctggc ttcgcggcgt caaaaatacc gagatacgag
    21781 tgatcgctgt tgctgtcgta gatgatgttg agcaggtatc ccaggttttg accaggtttg
    21841 cctgtgggca caaaaacagg ttcaccgccg aagtggccag gcggcagcgg agtgatttct
    21901 gagcggagct tccctttctg gatgtggaaa ctcacgtggc tgtcaaagat gccctctgtc
    21961 gcgttgcatt ccggccgcct gagggctgcg tacgcatact cgtacttctc acccgtcact
    22021 cggttgtcta tcaccggaaa ttcggcgaaa cctgcctgcc agagatcctg cttttccact
    22081 tttcctgtct gcagatcgat gcggaatctg ctgaaggcgc cgggatttac tcccgcgaaa
    22141 agttcttcgc gaatttcttc gtcgctgccc atcagcggaa ccttgtcgta aaagaaacca
    22201 tcgaggtaaa ttgccccggc atcttcataa gcgttcgcgt agtggtagac atagcccgct
    22261 ggcagctcca gggtgcgcag ggtctgtttc tcgccgctgc gctgcagcag gtagagaaca
    22321 gcgggtttat ccggctgcca ttcggcgatg cggccccgct gaatagacgc gagtgaacta
    22381 accgccggga aaaccggcag cagcgcatag ttgcgggtaa tcgcaaagtc gtgtatcatc
    22441 gcggcataag gagtttcgac cggagtccgg ctgatcagct tgcccgatgg gttgataacg
    22501 tagtattcca gccggggttt accaaatacc cggtagccga atgcgtacag gtagccggac
    22561 tgtgggtcta ccttggggtg ggctgtgaaa ggtgcattga agttactgcc gaaattctca
    22621 aagccttcgg tttcgagcga ggcgcctgaa agccgcagcg gcgggctgct ttcgaccaac
    22681 gcgtaaacat ggtctctgaa cggcaggatc gccgtattcg ccttgctaat gagcctggtg
    22741 tacgaatcgg cattaccgtg gcggataagc ccacccagta ggctgaaaac ttcaaatccg
    22801 accagctgcg cattcgccag cagggcaagg ttaggcccta agaacaggct cttgccggag
    22861 cgctgttcga aattaaaggt cggtgtgcgc gcccatgcat tgcggtatgc gacgccgccc
    22921 cgggcaaagt ggaagctatg gatcatccca tcacccagga accagtggtg gttggccgct
    22981 acgcgctgcg cgggattcgg tccgttgcgg tagagcgaac cgtgcaggtc gcgggggatc
    23041 gtgcccttga tatcaaaatt atccggcgtg ttcagggtca gttcttctga gatcggcgca
    23101 aaattgccct cgagaaaaat attgggctgc atggctttcg actgtaaact tgcgttctgc
    23161 ccgtcgatag ttttctgtct ctcttccggc ggcacaaacg cgagcatttc gttaaatggc
    23221 gaaatattgt ggatgcatcg gctgtatagc agatttgggg ggagcaggtg tccgttagcc
    23281 tgagcgtgcc acaggtacaa gggcgttttg ctattgtaag tctgttcaaa aatctggttt
    23341 tacccccatg tctctgcaat acccaaattt tctgtctccg ctcgacctgg gctttgccag
    23401 gttgcgtaac cgttcgctca tgggctcgat gcacacggcg ctggaagaaa ccgagaacgg
    23461 tttcgcgcgt ctcgcggctt tttatgctga gcgtgcgcgc ggcgaagcgg gcattatcgt
    23521 caccggcggt atcgcgccca acccggcggg acgggtcttt accggcgctg caaaaatgga
    23581 taccgaacac gaggccgacg aacaccgcgt gatcaccaag gccgtgcatg ataacggtgg
    23641 attaatagcg atgcaggtgc tgcatacagg gcgctatggc taccaccgcg actgcgtggg
    23701 agcatcggaa attcaggcgc cgatcaactt tgtgaaaccg aaaaaactga gccacga
//
LOCUS       DAOQJF010000006        21361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1117_length_21361_cov_5.562001, whole genome shotgun sequence.
ACCESSION   DAOQJF010000006 DAOQJF010000000
VERSION     DAOQJF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 21361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21361
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1117_length_21361_cov_5.562001"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLF85_00680"
     CDS             <1..855
                     /locus_tag="PLF85_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNE18195.1"
                     /translation="KSLAYIIRHYTKEAGVRQFEREIAQVARKIAREIVEKSDAARSF
                     ALDKQAIITYLGPAKYLYTRKESENPVGKVSGLAWTSAGGDILNIEVAFAHGKGSLTL
                     TGNLGDVMKESASTALGFVRSQAHYLGLSPDFFEKNDIFIHVPEGAIPKDGPSAGITL
                     VTAIVSALTQRGVLSEFAMTGEVTLRGNVLPIGGLKEKVLAAFRGGITHVICPKDNQK
                     DFEEIPQSIRQQMEFHFISHVREAVALALVQGQTIFQERAGFAFHATHLTGSIAAGST
                     PPATTQPM"
     gene            complement(868..1902)
                     /gene="add"
                     /locus_tag="PLF85_00685"
     CDS             complement(868..1902)
                     /gene="add"
                     /locus_tag="PLF85_00685"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNE18196.1"
                     /translation="MTDTKYLHALPKIDLHHHFDGAFDLKHLYQEAKRRNLPQGQLSE
                     TDFYARCQVSPSCRTLTEFLAVFEFFYEIAQDADFLHEEAHNLPARMAEQGVIYLETR
                     FGPHLFSGAGLTPERVTEAVLSGLERGALAGSMPPVRLILCAMRNAPVKQVEELLSLY
                     ERFHSRGVCGLDLAGDESRYACREYAPVFARAKEMGIPVTIHAGEAAGYESMLDALDL
                     FGARRIGHGIRAVESPALIERLAREKITLEVCLTSNLQTGNAAGYAEHPFEKLRSAGV
                     PVTINTDDPSVSGIDLGHEWQQAIRTYSLTAKDQKQLLLNAADAAFCSDAERDGLRNR
                     IENFFAALPR"
     gene            complement(1910..2800)
                     /locus_tag="PLF85_00690"
     CDS             complement(1910..2800)
                     /locus_tag="PLF85_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE18197.1"
                     /translation="MRTKIVKKKAPPKKKKSPPRQRYGLVLSGGAALGSYQAGAVRAL
                     FEAGLDIRCVAGSSIGALNGYLAATREIDRMIAFWKNIDQKKLITIQSFTKLFFSGST
                     SLLTDQMQRKLVEEAVNLDSLQKSGTTYICAAISINTGKLHYFRGNEAQTDRELRDYV
                     LASAAVPGVFPPVKIGEELYMDGGLVRNTPIEALESERVDSIIAISMEPEYYPEEKIG
                     NTAQIALRTLSIFFRSQADDAVARSLVSRRRRAKQLLLLRPQLPLDMQQYEFDPKKIE
                     RLIEQGYKAASQFIARHHLH"
     gene            complement(2803..3534)
                     /locus_tag="PLF85_00695"
     CDS             complement(2803..3534)
                     /locus_tag="PLF85_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNE18198.1"
                     /translation="MSTPTDFSKSELERYSRQILLYGKAGQQRLKDARVIVIGAGGLG
                     STFLPVLAASGIGNIVVYDADTVERSNLGRQLLYTEADLGKPKASAAADRLMAINPFI
                     QLQGIPRRFDETEAGALRTASLVFEGSDSLRTKFLLNDLCLSAGIPLVISALGNAQGH
                     TMLVSGSDSACYRCIFEEIDEQELPTCANEGILSAFPALVAAQAAHVAIRRILDANLP
                     AGFWVFEKSHCRNVSVRRRKDCLHP"
     gene            complement(3531..4571)
                     /locus_tag="PLF85_00700"
     CDS             complement(3531..4571)
                     /locus_tag="PLF85_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18199.1"
                     /translation="MWFPLLLFFALNFGAISAATGEECAFSLKQETPKHINFTVQSGR
                     IAFLCKSDGFFLRGTVTRTLGWQLTGKIFETMAERLKAETLEKWPSGRLYRIVGEKQT
                     EQKSLICGYHTRDKYSIDLCAPEGHGSPRVGAATDGKATTEGHGSPRGDDRARRLVSG
                     ILQEFRKISLEESPLLKSAPLYSQKKVFTEENETREPALLALSMPPGYVEVTRRDNRS
                     LFTDAAGLAEMEIFYELSDLALDSDLAHRVYRKSISAFLAKQGPWRPEREDNLEKSGE
                     ATCLLFTDNGQRLSHYCYMVVPVTVAGKKKFCRVAFVAAFLREAVDRSKLLAEQLALL
                     GDWAAVIRRHSN"
     gene            complement(4602..5099)
                     /locus_tag="PLF85_00705"
     CDS             complement(4602..5099)
                     /locus_tag="PLF85_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE18200.1"
                     /translation="MKKFAAVLLFAPILALSAKDNKEYIDDLKSADDGVAIAAAQYLG
                     NDGVKDAIEPLGEVVKSNRSAGVRIAAASALGKMDTKGRPTTLLREAIEVDQDNQVVY
                     TELLALLNLKDVDNADLTRAVEFCEKNKQSDIFISDIVARIRKVVPKKEAAPAKTEAP
                     AEAPK"
     gene            complement(5131..6096)
                     /locus_tag="PLF85_00710"
     CDS             complement(5131..6096)
                     /locus_tag="PLF85_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNE18201.1"
                     /translation="MLYSPARVISDTNKTIASILQGDLRILKKIAAYVVESGGKRIRP
                     LFMYYLGRACRIDSRELIELGALLEIVHAASLLHDDVIDGADERRSRPTGAKLFGNKQ
                     VVLGGDHLLSSGLKYLNGMQNSSYMTIFTDAIQALSAAELLQMQQHFNLKTSRKLHDR
                     VVDGKTAALFRAAGALVSVMRGEKDFYHTDIADLGLTLGRFFQERDDHLDYFDAARLK
                     KKGLQDFLNGIVTYPLLLLLSSANRTESGEIRAEWEFARLQGKIRNQERILNLMKKYD
                     VERRSADELRAMQKQILAGIGQLPEPKARQIISTEFEKILAVRSP"
     gene            complement(6145..7134)
                     /locus_tag="PLF85_00715"
     CDS             complement(6145..7134)
                     /locus_tag="PLF85_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE18202.1"
                     /translation="MAIVDRLKFNGGPDDIAWKFPDEGITWGAQLIVAESQEAVFFKG
                     GQCHDLFGPGTYTLKSNNIPLLEKFVNMAFGGDTPFAAEVWFVSKQIKQGIGWGTKTP
                     IQVEDPKFGIITNLRAFGVYGVRVADTRVFVKEVIGTQGKTTTESIQDLMLSTIVSDI
                     EEMLSQVVHEQNIDIIRINTQRSKIEEAFKPLLAKKFERFGLAIEQFEIESLNIPQDD
                     PGFKALQQAKADKAQMDILGDKYAQKRMLDIGEAGAKNEGAGGGMMQAGMGMGMGQMM
                     GQMMGQMNVSGQPAQPAADDPMAKIQKLKGMLDQGLITQEEFNAKKAKILETM"
     gene            7315..7764
                     /locus_tag="PLF85_00720"
     CDS             7315..7764
                     /locus_tag="PLF85_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE18203.1"
                     /translation="MRYSDALTVGDIMLTDVVTTRVGENVEDTYRSMLEGRFRHMPVV
                     GEGGKLVGIVSDRDLRNVLVFVNDPEGGRSVVGDKKLIIGKVMTRDPLRADREDSVRT
                     AVKLMTKHKVGCLPVCDENDRLLGIVTETDMLRLLEELLGKGKKPAK"
     gene            7952..8794
                     /locus_tag="PLF85_00725"
     CDS             7952..8794
                     /locus_tag="PLF85_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18204.1"
                     /translation="MKKILAATTLLAVFATSAAIFAQPAAKPADTAKKEEKKDNPADE
                     KEAKWYNPDYRPYVASFDELYKLGDDFANNKFRLALSNYQTGRSLMDKMREEVQRNKE
                     EWADAKYLNEKWYWQTIDRKMREERILLEKKNRAKIKAVTYFTRAINHLDEIQNRSFR
                     EKQQMKDLTAAVYRDWIIAQYDLGNLPQTIDLLERYLKIDPAFETEISPHKYLASAYG
                     FKEAVADKYNKGTEAERVFYKKNKNKHLLRATELKYKKDSPEYEHILELVNRDEIIAT
                     KPSP"
     gene            8930..9175
                     /locus_tag="PLF85_00730"
     CDS             8930..9175
                     /locus_tag="PLF85_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HNE18205.1"
                     /translation="MARIVELKIPDIGGNDGIEVVKWNKKAGENFVAGDELCELVTDK
                     AAFSLEAPKDGKLLEICVAEKAHPKVGELAARVEVAD"
     gene            complement(9242..9628)
                     /locus_tag="PLF85_00735"
     CDS             complement(9242..9628)
                     /locus_tag="PLF85_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18206.1"
                     /translation="MKTVSRVRFGLLAGVLVILLAGGLSAFGSSVVRLVREPAAVAFS
                     VQKGYGVQRDGKHELVFTAVSTGKVLKTVKGFKGAIATEDKKYFSRLEPVGLPAVTGK
                     VRVTGRIFYCSFEQKFCSVQRVDEEI"
     gene            complement(9685..10554)
                     /locus_tag="PLF85_00740"
     CDS             complement(9685..10554)
                     /locus_tag="PLF85_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE18207.1"
                     /translation="MHLQRARGTFFILCSALLFYLATFFVRLASERYTIPGSWYTLSR
                     FALGLPLFWFFFRRARTEVNNRTWLIVRAVCNVVAVVFFLYAAHFGSVMNANILNMTY
                     PAFVALLSPVFIGEKNSWTSWISVMAAITGATLITLGGKELELRNSDLLGLASGITAG
                     VGVMSLRQIRKTDSTFNVLYYTFLLGTLASILLVLLESFVWVQPAAGLAEPGFWSLLA
                     LSGLCGVLGQWVFTYGFAWVGAVEGSILSSTRILIALAFGVLWFGEAFTGAAALGAGL
                     VLFSNVLLSLRRA"
     gene            complement(10554..11240)
                     /locus_tag="PLF85_00745"
     CDS             complement(10554..11240)
                     /locus_tag="PLF85_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18208.1"
                     /translation="MIAVLVATRVEAQRLLRQLTPVKREGIFHYRGKLAGKPAVLFLT
                     RPGVGSREQVRRFLRLYNTDLVIVAGACGSLTSALRSLQAVQIGAVTNADREWLQLQG
                     GSATKCVSVDHLVSDDAEKALLRDRTGADILDMETWTIAKILSEAEFASRHFVAVRVV
                     DDLPGEENWLNKEQQLRDLAARRPSGRLNLAEIIRFGIWDFFSIRARRGRVALAIGRA
                     VTAVAAGKPV"
     gene            complement(11237..11902)
                     /locus_tag="PLF85_00750"
     CDS             complement(11237..11902)
                     /locus_tag="PLF85_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNE18209.1"
                     /translation="MRRRLVVTSGPTREFFDPIRFLSNPSTGRMGHSVAAAAVRRGIF
                     DVVFISGPVMPEFATVEGARNVSVISTDDMLAAVGEALTPGGILVMAAAPADYKPAVK
                     SPVKIKKTENPQISLVPNPDILKEMARRNTTLSPRALLIGFAAETHETEKYALGKLRD
                     KQLDMIFLNDVSRQGAGFASTTNEFTVFYRDGRRLELANAVKEALGEKIIDCVLEYAE
                     QQP"
     gene            complement(11929..12336)
                     /gene="arsC"
                     /locus_tag="PLF85_00755"
     CDS             complement(11929..12336)
                     /gene="arsC"
                     /locus_tag="PLF85_00755"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801920.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNE18210.1"
                     /translation="MAKLSGAPPQGTTNVAAGAHAKVTIYYNPKCRKSREALQIIKEK
                     GIDPEIRNYMEEPPSKAELSEVLRKMGRRPRDIFRKSEPLYKDLGLKNKDLTDDELLT
                     YLNEYPILIERPIVVKGQRAVLGRPPEDVRRII"
     gene            12481..14772
                     /locus_tag="PLF85_00760"
     CDS             12481..14772
                     /locus_tag="PLF85_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE18211.1"
                     /translation="MRENTASETIAEQSLWKVRLLRRAIITVLAGSLIMISMWIIRMG
                     RIDGAAAFAILLTLPNAVVLHPGFGWRAKGVLLVTQLTLVGLYFPLHLGVRYTALSIA
                     GAAVMITATFFGRRVALVLLAIDAIVISSYGFLSQLGYLAIDVQPLPGDTVLSTWLKV
                     VVVFVAVGAMLVMLISGIVDSLQVSIRRLRRVSEIAATDRRYIRQLIDSAPEAIVVFD
                     MRQGRFIDVNPQAEKLFGHSAARLKEMTPADLSPPLQENGRPSAELAREYLSRAADGE
                     MPVFEWLHTNSSGESIPCEIRLHRMPVEGAVQIRGSITDIRERRKSQAIIHSLALYDN
                     LTGLPNRKLFQDRLRHTIAASERDQQYSALFFIDVDNFKNINDTSGHASGDFYLTVVA
                     ERLSTCVNEGDTVARWGGDEFVVIAENLTGSLSEAARRAELMGEKMIAALAEPCENPY
                     ARGQYFQNTVSVGLTLLYGHIHSPEELLKRADIAMYQAKMSGKNRLRNFDVRMQREIE
                     QKLAFENDLRRAVAAGEFQLYYQPQVDAARVIFGAEVLLRWFHPTRGQVLPGDFIALA
                     EETGDILPIGRWVIEAACKKLAQWQRDERTKHLVLSLNVSPRQFRDPQFVDYVTQAVR
                     DTGINPRGLKLELTEGLMLDNIEETIGKMEALGKTGLSFCLDDFGTGYSSLSYLKRLP
                     LSQIKIDQSFVRDIEVDANDAVLIRTIIGMANNLNLGVIAEGVETEEQWQMLRAMGCS
                     AYQGFYFGRPAPAAELEALAAKP"
     gene            complement(14779..16098)
                     /locus_tag="PLF85_00765"
     CDS             complement(14779..16098)
                     /locus_tag="PLF85_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18212.1"
                     /translation="MKASYRLAAALLAALTLGALVYAQDNTADDDTYPVRIILEPRPD
                     ARFYQVEWLEGNEISGEEEAPTGSSLREKIKRTEIRRNLPLRFRYFRLRSAYRDGLYG
                     PWGEVVEIQRPTRVAKKDKKKPDTGAGKATGTEPATGNQQGKDTAAAVKPAKKKSSTK
                     HSEEAKPLEPQDVFAQVIDKDGKEKWVLRGKSVAAERLDETTPLWYDVECLTERDAPE
                     NTNGRRRYDGPVPFTRAGQYRMNLYNSDDNSQNKPLQTWVFWVYTDVPRTYVKFYAPF
                     LHGRGGFIVGGKTKISLSSQFAGAAIDRIDYRVFAEGATPGAWQKYEDDVEVSSFAQG
                     HYGYYNIEYRATNVAGSVEPPQIRRMLIDSRGPAIEERAGEDGSAGFTFTDEHFPIVV
                     RIYQNGTLVQDMYFKQWKANETVRAPAGAGYEIHAIDLLGNETVLKK"
     gene            complement(16201..17259)
                     /locus_tag="PLF85_00770"
     CDS             complement(16201..17259)
                     /locus_tag="PLF85_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18213.1"
                     /translation="MRALKQAIPGSFFRSLILSSLLSFVCATAATASGKVRDVALQRN
                     QVTVTMSKAGVFALGKKIYFYRNGKATGSAEVTQAFHTKAIARILTGSPQAGDDASAT
                     AKAPKQQAKINRVSFAASSVQAQEFLVELQFENQEKIQRKLTLNAELAVLIKGAPTYM
                     GLSAALLKRVDLLWENGALRVTGVELSDKSRFELAGLVASDLYTRIKPAAETRNSTVP
                     GKIIFLKKVRDLPQLADGVEIELRASAESERIKKGTIYKLKVYCNELFMTEFLLKPEG
                     SDLADQLTIAPVDLAATENNLEFRLVEVTDSGELLLETDNNQLIAMLALEGTRPDRNT
                     RVRVTLGKGFDAFANMLK"
     gene            complement(17247..18731)
                     /locus_tag="PLF85_00775"
     CDS             complement(17247..18731)
                     /locus_tag="PLF85_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE18214.1"
                     /translation="MRYNLFLVFSLLVPTAFIHSLTVGVFDIRGDVGSEGGKLADLAQ
                     SEIQAEKSLTLRDRLSIKTLIEQQEKCAAGYKACPDLGGSAKTLDVYITGEAISLSAQ
                     KQVILRAVRTNDQKIAAIAVGNGDNLEKSMTSAAAELRKKMSGIAQNDGGASDRWRLA
                     VQPVRAANEAAQKLPELAGLDSMLLGALSKKASFDLVETKSDDIVEAEKMLVLSGFAP
                     GAMAPVGGDYTHFVTATIKVQDEARILAYQVISRKTGVAVISDIIEWRADENPQKALD
                     KVADYTENDLMRVLGKLEIAGCDPADAMLSFEFKDPARAPEQIQCKSPLLVEDIPAGE
                     YTIVFRHEDRNTLSKTITIKPVETLKLGKIQLPDIDLSLFRNGSAAESAGNFGEANSL
                     YSQFYNKYPRHRMASYAMYREGYVTQLRLKKVAEGRKILENVIARKPDAEIRSEAYVG
                     MALGYQAEGDAEKANNIFRMLTDQYAGTTAAEFARECLEGKCAL"
     gene            18886..19566
                     /locus_tag="PLF85_00780"
     CDS             18886..19566
                     /locus_tag="PLF85_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18215.1"
                     /translation="MVTLEQLEQLEGRIVKALELISDLRVENSHLESEAERLKASNDQ
                     LRLTAEEREREAQKWQQQLQEATQELSRLKSKEQELETRILSIIAKLDNLKGADKPAR
                     SESVVSATPANAPAPAVEPVKAESSAAPVAAAAAVGAGTGAAAVAANATLNEKDEIVI
                     DEEDDEIILLEDEEEFVSEKKTEEKKEAKAPADEIIIEDDDVLGVFDDDDDEDFLIVE
                     EEMSDRKK"
     gene            19580..20080
                     /locus_tag="PLF85_00785"
     CDS             19580..20080
                     /locus_tag="PLF85_00785"
                     /inference="COORDINATES: protein motif:HMM:NF027443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4833 domain-containing protein"
                     /protein_id="HNE18216.1"
                     /translation="MKSLILLLVAAFFSGGLFAESRSNLFHIKRNKNRNEVHYAVRYD
                     ENTCKPIENEPLYGYWLMLEKGPNVFEPIGRLERMAYGIQSQEIKGNDLVVQLKAFPD
                     RAIRITFNAAGGCKITPFLPINGQEAVLKFISVFAEEGLIKPTVKHVDISGVKGGTPV
                     SERINK"
     gene            20126..20698
                     /locus_tag="PLF85_00790"
     CDS             20126..20698
                     /locus_tag="PLF85_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNE18217.1"
                     /translation="MPVTGPIALIVFRSSMRGHFSRALRVVAGAAVAEVIYCALATFG
                     YVQIVAAYPFLAKYIRYVGASFLVVLGIVFMFQKVHLSEESAPVSERKSAGLVSGFLI
                     AILNPTLFLTWGSASSTIFSWFDSIGFWDMVLFPIFAGLGILTWFTILLEIFKKYREQ
                     IGEKIGFYAIRGAAVVMIASGTYLLTQAGK"
     gene            20695..>21361
                     /locus_tag="PLF85_00795"
     CDS             20695..>21361
                     /locus_tag="PLF85_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE18218.1"
                     /translation="MRTGSCCLPLLLLALQCGSAPVTLGDKSIEPVYEQVLRDSAIKK
                     RLHQVDFDKKSFSIEDLFLLSLDRTEQLAIAAERGVAADAKVRRSYGAWLPRVSLVAT
                     QYAPVSPGFAVPGIRFAARQNIMTGLTEYTGIVGARLERVAEEQSLRAEMAAHLLNIA
                     DAALQYQLCLQLLHEAREAVRITESGLAEIRRRVALGRNKRGDALKTEARLRQKQADL
                     LAAE"
BASE COUNT         4595 a         5858 c         6023 g         4885 t
ORIGIN      
        1 aaatccctcg cctatatcat acgccactac acgaaagaag ccggtgtgcg ccagtttgag
       61 cgtgagattg cgcaggttgc ccgcaagatt gcccgcgaaa ttgtggaaaa atctgacgcc
      121 gctcgttcgt ttgctctcga taaacaggca attatcacgt acctcggacc ggcgaaatac
      181 ctctataccc gcaaagagag cgagaaccct gttggtaagg tcagcggtct tgcctggact
      241 tcggctggcg gagatatttt aaatatcgaa gtcgcttttg cgcacggcaa aggcagcctc
      301 acgctgaccg gtaacctggg cgatgtgatg aaagagtccg cgagtaccgc tctcggtttt
      361 gtgcgctcgc aagcgcatta cctggggctc agcccggatt tttttgaaaa gaacgatatc
      421 tttatccacg tgcctgaagg ggcaataccc aaagacgggc cttccgccgg aatcactctc
      481 gtgacagcga ttgtttcagc actgacgcag cgtggtgtgc tcagcgaatt cgcgatgacc
      541 ggcgaagtca cgctgcgcgg caatgtgctg cccatcggtg gtctcaagga aaaagtgctg
      601 gcggccttcc gtggtggcat tacgcatgtg atctgcccaa aagataacca gaaagatttt
      661 gaagaaatac cgcaaagcat caggcagcag atggagttcc attttatctc gcatgtacgc
      721 gaggcggttg ctcttgcgct cgtccaggga cagacaatct tccaggaacg ggcgggcttt
      781 gctttccatg cgactcacct gaccggttcg atcgccgccg gcagtacgcc gccggcgact
      841 acccagccca tgtaattggc tggcccgtta gcggggcagg gccgcaaaaa aattttcgat
      901 acgatttctt aacccgtcac gctcagcgtc agaacagaag gcggcatctg ccgcattgag
      961 cagcagctgc ttctggtctt ttgccgtgag cgagtaggtc ctgatggcct gttgccactc
     1021 atggcccagg tcgattcccg aaacgctggg atcatccgta ttgatagtga ccggcactcc
     1081 ggcagagcgc agtttttcaa acggatgttc agcgtaacca gctgcattgc cggtctgcag
     1141 gttactcgtc aggcacactt cgagagtgat tttttcacgc gcgaggcgct ctatcaatgc
     1201 cggactttct accgcccgaa taccgtggcc aatacgccgt gcaccgaaaa ggtcgagcgc
     1261 atcgagcatc gactcgtacc cggctgcttc gccggcatgg atggttactg gtatacccat
     1321 ctcttttgcg cgcgcaaaga ccggcgcgta ttcgcggcag gcgtagcgcg attcatcgcc
     1381 tgcaagatcg agaccacaga cgccgcgtga gtgaaatctt tcgtagaggg ataaaagctc
     1441 ttcgacctgc ttcacgggtg cgttgcgcat tgcgcagaga atcagcctca ccggcggcat
     1501 actacccgcc agtgcgccgc gctcgaggcc gctgagcact gcttcggtca cacgttcagg
     1561 agtgagaccg gcgccgctga aaagatgcgg cccgaatcgg gtttcgaggt agatgactcc
     1621 ctgttcggcc atgcgcgctg gcaggttatg tgcctcttcg tgcagaaaat cggcatcctg
     1681 tgcaatttcg tagaagaatt cgaataccgc cagaaattcg gtgagggttc ggcaggaagg
     1741 gctgacctgg cacctggcgt agaagtccgt ctcggacagt tggccctgcg gcaggttgcg
     1801 ccgctttgct tcctggtaga ggtgctttag gtcaaaagcc ccgtcaaagt ggtggtgcag
     1861 gtcaattttc ggcagtgcgt gcaggtactt cgtgtcggtc ataaatcccc tagtgcaggt
     1921 ggtggcgggc aataaactgc gaagcggctt tataaccctg ctctatcaac cgttcaattt
     1981 tttttgggtc aaattcatac tgctgcatat cgagtggtaa ttgcggccgt agtaacagca
     2041 gttgcttcgc ccgcctgcgc cgcgaaacca gtgaacgcgc aacggcatca tcggcctggc
     2101 tgcgaaaaaa aatactcagc gtgcgcaggg caatctgcgc ggtgttgcca attttttctt
     2161 cggggtagta ttcgggttcc atgctgatcg cgataataga atcgacgcgc tcgctctcga
     2221 gtgcttcgat gggcgtattg cggacgaggc caccatccat gtagagttct tctccgatct
     2281 ttacgggtgg aaacacgccc ggcaccgcgg ccgacgcgag cacatagtcg cgcagctcgc
     2341 ggtcagtctg ggcttcgttg cccctgaagt agtgcagctt cccggtattg atgctgatcg
     2401 ctgcgcagat gtaagttgtc ccggattttt gcagtgaatc gaggtttacc gcctcctcga
     2461 cgagtttacg ctgcatctgg tctgtcagca gcgaggtgct gcctgaaaag aacaacttgg
     2521 tgaatgactg gatggtaatc agctttttct ggtcgatatt cttccagaag gcgatcatac
     2581 gatcaatctc acgcgtcgcc gcaaggtaac cgttgagcgc cccgatcgaa ctgccggcca
     2641 cacagcggat atccaggcca gcttcgaaca gcgcacgaac agcacctgcc tggtaagaac
     2701 caagtgctgc tcccccggaa agcacgagac cgtagcgttg ccgtggcggg cttttctttt
     2761 tcttaggggg agcttttttt ttcacaatct tggtgcgcat attcacgggt gcaggcaatc
     2821 ttttcttctg cgaacgctta cgtttcggca atgacttttc tcgaaaaccc aaaacccggc
     2881 aggcaggttt gcgtccagga ttctgcggat tgcgacgtgt gcggcctgcg cggccacaag
     2941 cgcgggaaaa gcgctgagaa ttccttcgtt ggcgcaggtt ggtaattcct gttcatcaat
     3001 ttcttcgaag atacagcggt agcaggctga atcgctgcct gagacgagca tggtatgccc
     3061 ctgtgcgttg cccagtgctg aaatcaccag gggtataccc gcgctgaggc acaggtcgtt
     3121 cagcaggaat ttggtgcgca ggctgtcgct gccttcaaaa accagactgg ctgtgcgcag
     3181 cgcccctgcc tctgtctcgt caaaacggcg cggtataccc tgcaactgga taaatggatt
     3241 gatcgccatg agccgatcag cggccgcgct ggccttgggt ttaccgaggt cggcttcggt
     3301 atagagcagc tgcctgccga ggttgctgcg ctcgactgta tctgcgtcat agacgacaat
     3361 attgcctata ccgcttgccg ccagcacggg caggaacgtt gagccgaggc caccggcacc
     3421 gatcacgatg acgcgcgcat ctttcaggcg ttgctggcct gcctttccat agagcagaat
     3481 ttgccggctg tagcgctcga gctcgctttt gctgaagtct gtcggcgtac tcaattcgaa
     3541 tgcctgcgga ttaccgccgc ccagtcgccc agcagggcaa gttgctcagc gagcagcttc
     3601 gaacggtcca ctgcttcgcg caagaacgct gcgacaaaag ccacacggca gaatttcttt
     3661 tttccggcta ctgtaacagg tactaccatg taacagtaat gactcaggcg ctggccgttg
     3721 tcggtgaaga gcaggcaggt tgcctcgccg ctcttctcga ggttatcttc gcgttctggc
     3781 cgccatgggc cctgttttgc caggaaagca gaaattgact tgcggtagac cctgtgcgcg
     3841 agatccgaat cgagcgccag gtcagaaagt tcataaaaaa tttccatttc agccaggccg
     3901 gccgcgtcag tgaagagcga ccggttatcg cgccgggtga cttcgacgta acccggcggc
     3961 atggatagcg cgagcagtgc cggttcgcgc gtttcgttct cttcggtgaa gacttttttc
     4021 tgcgaataca gcggtgctga tttcagcagt ggcgactctt ccagcgaaat tttgcggaac
     4081 tcctgcagga tgcctgagac aagccggcgc gcacggtcat cgcccctcgg acttccatgt
     4141 ccttcggttg tcgctttgcc gtccgtggca gcgccaaccc ttggacttcc atgtccttcg
     4201 ggagcgcaga ggtcaatcga gtacttgtcg cgcgtgtgat aaccgcagat gagtgatttt
     4261 tgttcggtct gcttttcgcc tacaatgcgg tagagcctgc ccgacggcca tttttcgagc
     4321 gtctcagctt tcaggcgctc agccattgtc tcgaaaattt tcccggtcag ctgccagccc
     4381 agcgtacgcg tcactgtacc gcgcagaaag aaaccatcgc tcttacacag gaaagcgata
     4441 cgtccggact ggacggtgaa attgatgtgc ttcggagttt cctgtttcag tgaaaaagcg
     4501 cattcttcac cggtagcagc tgaaattgcc ccaaaattca gggcaaaaaa aaggaggagt
     4561 ggaaaccaca tcctccgcac tttgtgcgac cgtggcaccg gctatttcgg agcttcggca
     4621 ggcgcttcag tttttgctgg cgcagcttct tttttcggca cgactttccg gatgcgcgct
     4681 acaatgtcgc tgatgaaaat atcagattgt ttgttcttct cgcagaattc aacggcgcgt
     4741 gtcaggtctg cattatctac gtctttcagg tttagtagcg ccagcagttc tgtatagact
     4801 acctggttat cctggtcgac ttcgatcgcc tcgcgcagca gcgtagtcgg gcgccctttg
     4861 gtgtccatct tgcccaaagc agaagctgct gcaatacgca cgcctgcaga acggttactt
     4921 tttacgactt cacccagagg ctcgatggcg tctttgacgc cgtcattacc caggtattgg
     4981 gccgcagcga tggccacacc gtcgtccgca gatttgaggt catcgatata ttccttgttg
     5041 tcctttgcag aaagggcgag gatgggcgca aacagcagca cagcagcaaa ttttttcatg
     5101 ggcatattcc tttgaggatg ttaatgcgtt ttaaggcgag cgcacggcaa ggattttttc
     5161 gaactcggtg cttatgatct gccgggcctt gggctcgggt aactggccga tacctgccag
     5221 tatttgcttt tgcatcgccc gcagttcgtc tgccgagcgc cgctcgacgt cgtatttttt
     5281 catcaggttg agtatacgtt cctggttgcg gattttcccc tgtaaccggg caaactccca
     5341 ttcggcccgt atttcgccgg actcggtacg gttggccgaa gatagcagca gcagcagggg
     5401 gtaggtaaca ataccgttca ggaagtcctg caggcccttc tttttgagcc tggcggcgtc
     5461 gaaatagtcc aggtggtcgt cgcgttcctg gaagaaacgg ccgagggtaa gccccaggtc
     5521 agctatatca gtatgataga aatctttctc gccgcgcatg acggaaacca gcgcgcctgc
     5581 cgccctgaac agcgccgcgg ttttgccgtc gaccacgcga tcgtgcagct tgcggctggt
     5641 cttcaggttg aagtgctgct gcatctgcag cagctcagcg gcagaaagcg cctggatcgc
     5701 atcggtgaaa atggtcatat aagatgaatt ctgcatgccg ttcaggtact tcagacctga
     5761 agacaagagg tggtcgccac ccaggacgac ctgcttgttg ccgaatagtt tggccccggt
     5821 agggcgagac cggcgttcgt cggccccgtc gatgacgtcg tcgtgcagca ggctggccgc
     5881 gtgcacgatc tcgagcagtg cgccgagttc aatcagttca cggctgtcaa tgcggcaagc
     5941 gcgcccgaga taatacatga acagcgggcg aatacgtttt ccgccgcttt ccacgacata
     6001 cgcagcaatc tttttcagaa tccgcaggtc gccctgcaaa atcgaagcta tggttttatt
     6061 cgtgtctgaa atgacacggg ctggagaata taacactctg cggaatagtc gcgctccgca
     6121 gcctgggcca cggagcgctg tttcttacat cgtttcgaga atctttgcct ttttggcgtt
     6181 gaactcttcc tgggtgatca ggccctggtc gagcattccc ttgagcttct ggatcttcgc
     6241 catcgggtcg tcggccgcag gctgtgccgg ttgaccgctg acgttcattt ggcccatcat
     6301 ttgacccatc atttggccca tgcccatgcc catgcctgcc tgcatcattc cgccgccggc
     6361 gccttcgttt ttcgcgcctg cttcgccgat atcaagcata cgcttctgcg cgtacttgtc
     6421 gcccaggata tccatctgcg ctttgtcggc ttttgcctgc tgcagcgctt tgaagcccgg
     6481 atcgtcctgg ggaatattga gcgattcgat ttcgaactgc tcgatcgcga ggccgaagcg
     6541 ctcgaacttt ttcgcgagca gcggtttgaa cgcttcttcg atttttgaac gctgcgtgtt
     6601 gatgcggatg atgtcgatgt tctgttcgtg caccacctgc gacagcatct cttcgatatc
     6661 tgagacaatc gtgctcagca tcaggtcctg gatactctca gtggttgtct tgccctgtgt
     6721 accgatgact tctttaacga agacgcgggt gtcggcaacg cgcacaccgt agacgccgaa
     6781 cgcgcgcagg ttggtaatga tgccgaattt cggatcttct acctggatgg gggttttagt
     6841 gccccagcca atgccctgct tgatctgctt cgaaacaaac cagacctctg ctgcgaaagg
     6901 cgtatcgccg ccgaatgcca tgttgacgaa cttctcgagc agcgggatgt tgttcgactt
     6961 aagggtatag gtacctggcc caaagaggtc gtggcactgg ccgcccttaa aaaatacagc
     7021 ttcctgagat tcagcaacaa tcagctgagc tccccaggta ataccttcat cggggaactt
     7081 ccacgcgatg tcgtccgggc cgccgttaaa ttttaatctg tctacaattg ccatgcgaat
     7141 ctcctcttca gtaaatctct acctgatgcc ggcagggcat tttattgcca cggccgcacc
     7201 aagtgcaagt aacacaattg ccatgacact gccaagaatt tttattattc atgcgccagt
     7261 tgattctcgc agcctttgtt gactgcccga gccgccaccg cgaaaggaca atttatgcgc
     7321 tattctgacg ccctgacggt gggtgacatt atgctgaccg atgtcgtcac gacgcgcgtt
     7381 ggtgaaaacg ttgaagacac ctaccgcagt atgctagagg gccgctttcg ccacatgccc
     7441 gtggtgggcg agggaggcaa actggtgggt attgtttctg accgtgacct gcgtaatgtc
     7501 cttgtgtttg tcaacgaccc cgaaggtggg cgttccgttg taggcgacaa aaagctgata
     7561 atcggcaagg tgatgacgcg cgacccgctg cgcgctgacc gtgaagattc tgtgcggact
     7621 gccgtgaaac tcatgaccaa acacaaggta ggttgtcttc cggtttgcga cgaaaatgac
     7681 agacttctgg gtattgtcac cgaaactgac atgctccggc tactggagga gctgctcgga
     7741 aaaggcaaga aacctgcgaa gtgacataat ttgaccggct ccgcgccaaa aaattaccgg
     7801 gtaatgcagc acaattcaat ttaagattta cgcatcgaac gccgacaaag aatatagtgt
     7861 attatccgca ggatttggtt ttgcagcatg gctgccatac caaatcctgc agagactgac
     7921 acgacttgaa ggcgacagaa ggagaattat tatgaagaaa atactggcag cgactacact
     7981 gctcgcagta ttcgcgacct ctgcagcgat tttcgcgcag ccggctgcga aaccggcaga
     8041 taccgcgaaa aaagaagaaa agaaggataa ccccgcagac gaaaaggaag cgaagtggta
     8101 caatcctgac taccgcccat acgtggcatc cttcgacgaa ctttataaac tcggcgatga
     8161 ctttgctaac aacaagttcc gcctggcact gtcaaactac cagacaggcc gttcgctcat
     8221 ggataaaatg cgcgaagaag tacagcgtaa taaagaagag tgggcagatg cgaagtacct
     8281 gaacgaaaag tggtactggc agactatcga ccgcaagatg cgcgaagagc gtattctgct
     8341 cgagaagaaa aaccgcgcga aaatcaaggc ggtaacgtac tttacccgtg cgatcaacca
     8401 cctcgacgaa atccagaacc gctcattccg cgaaaagcag cagatgaaag atctgacagc
     8461 agcggtatac cgcgactgga ttatcgcgca gtacgaccta ggcaacctgc cgcagaccat
     8521 cgacctgctc gagcgttacc tgaagatcga tcctgcgttt gaaacagaaa tttcacccca
     8581 caagtacctg gcttcagcct acggcttcaa agaagcagtt gctgacaagt acaacaaagg
     8641 cacagaagca gagcgcgtct tctacaaaaa gaacaagaac aagcacctgc tgcgcgccac
     8701 agagctcaaa tacaagaaag attctccaga gtatgagcac attctggagc tcgtaaaccg
     8761 cgacgagatc atcgcgacaa aaccatctcc gtgatgtaac ccttcgcgcc gcaaggcagt
     8821 tgcccgaagg tcaaaacccc gcgcaagcgg ggttttttta tatccacctg gaaggcagtt
     8881 catggaaatc tactttacgc gcggcaggcg agcgcaagct aatagccgca tggcacgcat
     8941 tgttgaactc aagatacccg atatcggcgg taacgacggc atcgaagtgg tgaagtggaa
     9001 caagaaagca ggtgaaaatt ttgttgcggg cgacgagctc tgcgagctcg ttacagacaa
     9061 ggctgccttc tcacttgagg cgcccaaaga tggtaaatta cttgagattt gcgttgcgga
     9121 aaaagctcac ccgaaggtag gcgagctggc ggcgcgcgtc gaggtagcgg attagcaggt
     9181 ttcggaaaaa cttccgcgcc ggctacagac gctcgctgag tcgcgagtca gcgagcgcaa
     9241 atcagatctc ttcgtcgacg cgctgcactg agcaaaactt ctgctcgaag ctgcagtaga
     9301 atatgcgacc cgtaacgcgc accttgccgg tcacggctgg caatccaacg ggttcaaggc
     9361 ggctgaagta cttcttatct tcggtcgcaa ttgcgccctt gaagccctta acggttttca
     9421 gcactttgcc ggtcgatact gcagtaaaga ccagttcatg cttaccgtcc cgctgcacac
     9481 cgtagccctt ctgtaccgaa aacgccacgg ctgccggttc gcgcaccagg cgcacgacgc
     9541 ttgacccgaa agcagaaagg ccacccgcca gcaaaataac cagaacccct gccaacaggc
     9601 cgaaccttac tctactaacg gttttcatac ttttaggata agcaatccgc caatgaggct
     9661 gccacaaaaa taccgcctgc gtgtctatgc gcgtcgtaag gacagcaaca cattggagaa
     9721 caggaccaaa cccgcaccga gcgccgctgc cccggtgaag gcttcgccaa accagagaac
     9781 gccaaaggcc agtgcaatca gaatgcgcgt cgacgacagt attgaacctt cgacggcgcc
     9841 gacccacgca aagccgtagg tgaagaccca ctgtcccagt acgccgcaca gccccgaaag
     9901 cgcgagcagg gaccagaaac ccggctcagc cagccccgcg gccggctgca cccagacaaa
     9961 actttcgagc agtaccagca gtattgatgc caatgtacca agcagaaagg tatagtagag
    10021 cacgttgaag gttgaatcgg tcttgcgtat ctgccgcagg ctcatgacgc ccacgcctgc
    10081 cgtgatgcct gatgcgaggc ccagcaggtc ggagttcctg agctccagtt ctttaccgcc
    10141 cagggtaatc agcgtggcgc ccgtgattgc cgccatgacg gatatccagc tggtccagga
    10201 attcttttcg ccgatgaaca ccggcgatag cagcgccacg aaagccgggt atgtcatgtt
    10261 gagtatattg gcgttcatca ctgaaccaaa gtgtgccgcg tagagaaaga aaaccaccgc
    10321 aaccacgttg cagaccgcgc ggacgattag ccaggtgcgg ttattcactt cggtccgcgc
    10381 gcgccggaaa aagaaccaga agagcggcaa tccgagcgcg aagcgcgaga gcgtgtacca
    10441 tgatccgggt atcgtgtacc tctcgctcgc cagcctgacg aaaaaagtcg caaggtagaa
    10501 aagcagcgcg gaacagagta tgaagaaggt gccgcgcgcc cgctgcaggt gcatcagaca
    10561 ggttttcctg cggcgaccgc tgtgacagcc cgcccgatgg caagcgctac gcggccgcgc
    10621 cgtgcgcgaa tagagaagaa atcccagatg ccgaaacgaa taatttcagc gaggttaagc
    10681 cggccagaag gtctgcgtgc ggcaagatcg cgcagctgct gctctttgtt taaccagttc
    10741 tcttcgccgg gtaagtcatc gacaacgcgc acggcaacaa agtggcgcga agcgaattcg
    10801 gcttcgctca gaattttggc gatggtccag gtttccatgt cgagtatgtc tgcgcccgtg
    10861 cggtcgcgca gcagcgcctt ttcagcatcg tcgcttacaa ggtggtctac agaaacgcat
    10921 ttggtcgcgc tgccgccctg cagctgcagc cactcacggt cggcattggt cactgcgcca
    10981 atctgcaccg cctgcagcga gcgcagtgcg ctggtcaggc tgccgcaggc gccggctaca
    11041 atgacgaggt cggtattgta gaggcggaga aaccggcgga cctgctcgcg tgagccgaca
    11101 ccggggcgcg tcaggaatag tactgccggt ttgccggcga gtttgccacg gtaatggaaa
    11161 atgccttcgc gcttcacggg tgtcagctgg cgcagcagcc gctgcgcctc aacccgggtg
    11221 gctacgagta cggcaatcac ggttgctgtt ctgcgtattc gaggacacag tcgataattt
    11281 tttcgcccag cgcttctttc accgcattgg cgagttcaag ccggcgaccg tcgcggtaga
    11341 agactgtgaa ttcattcgtg gtcgaggcga aacctgcacc ctggcgggac acatcgttga
    11401 gaaagatcat gtcgagctgc ttgtcgcgca gtttgcccag ggcgtatttc tccgtttcgt
    11461 gcgtctcggc cgcaaaaccg atcagcagcg cgcgcggcga cagggtagta ttacgccgcg
    11521 ccatttcttt taggatgtcc ggattcggga ccaggctgat ctgagggttc tcagttttct
    11581 tgattttgac cggtgatttg accgcaggtt tgtagtcagc gggtgctgcc gccatcacca
    11641 gtattccgcc cggcgtcagt gcttcgccga cggcggccag catgtcgtcg gtactaatga
    11701 ccgaaacatt acgagctccc tcgactgtcg caaattcagg catgactgga cctgaaatga
    11761 aaacaacatc aaaaatgccc cgcctgacgg ccgcagcggc cacggagtga cccatacggc
    11821 cagtactcgg gtttgagaga aatcggatgg gatcgaaaaa ttcgcgcgtg ggccccgagg
    11881 tgactaccag cctgcgccgc atattacccc gggcctcaag cccttacgtc agatgatgcg
    11941 acgcacgtct tcgggtggac gcccgagtac tgcgcgctgg cctttcacga caatgggccg
    12001 ctcgatgaga atggggtatt cgttcagata ggttaacagc tcatcgtcgg tcaggtcttt
    12061 gtttttcaga ccaaggtctt tatacagtgg ctctgacttg cggaaaatat cgcgcggacg
    12121 gcgacccatt ttgcgcagca cttcagagag ttctgccttt gaaggcggct cttccatgta
    12181 gttgcggatt tcagggtcga tgcccttttc tttaataatc tgcagagctt cgcggctctt
    12241 gcggcacttc ggattataat agatggtaac ttttgcgtga gcgccggctg ctacgtttgt
    12301 tgtcccctgt gggggagcac cagaaagttt cgccatgccg acagaaatcg cgtttgagac
    12361 atgccgtcaa tgagaaagag cttttcagcc agccactaaa tgcgtaggga atagacaaat
    12421 tgctgggaat ttaatcccgc tttacagaaa gcgggcaatt atagaaaaag aaagccggtg
    12481 atgcgcgaaa acactgcaag tgagacgatc gcagagcaaa gcctgtggaa ggtgcggctg
    12541 cttcggcgcg cgatcatcac agtcctcgcc ggcagcctca ttatgatctc gatgtggatc
    12601 attcgcatgg ggcgtatcga tggcgcggcc gcctttgcca tcctgctgac tttgcccaat
    12661 gccgttgtcc tgcatcccgg gtttggctgg cgcgcaaagg gagttctgct tgttacgcag
    12721 ctgacgctcg tcgggcttta ttttccgctg cacctcggcg tgcgctacac agcgctcagt
    12781 atcgccgggg ctgccgtgat gattacggcc acattctttg gccgacgcgt tgcccttgta
    12841 ctgctcgcta ttgatgctat agttatttct tcttatggtt tcctctcgca gttgggttac
    12901 cttgcgatcg acgtccagcc gctgcccggt gacacggtgc tgtcgacctg gctcaaggtg
    12961 gtggtcgtat ttgtcgcagt gggagctatg ctcgtcatgc tgatatccgg cattgtcgat
    13021 tccctgcagg tgtcgatacg aagattgcgc cgtgtttcag aaattgccgc gactgaccgg
    13081 cgttacatca ggcagctgat cgactcagcg cccgaagcga tcgtcgtctt cgatatgcgg
    13141 caggggcgtt tcatcgacgt caatccgcag gctgagaagt tgttcggcca cagtgcggcc
    13201 cggctgaaag agatgactcc tgctgatctc agtcctccgt tgcaggaaaa tggcagaccg
    13261 tcggccgagc tcgcccgcga atacctgtcg cgtgcagctg atggagaaat gccggtcttt
    13321 gagtggctgc atacgaacag ctcgggtgaa tctatacctt gcgaaattcg cctgcaccgc
    13381 atgccggttg aaggcgcggt gcagatacgt ggttccatca ccgacatccg tgaacgcaga
    13441 aagtcccagg cgataatcca ttcgctcgcg ctttacgaca acctgacagg gttacccaac
    13501 cgcaaactct tccaggatcg cctcaggcac acgattgccg cgagcgaacg cgaccagcaa
    13561 tacagcgcgc tgttctttat cgacgtcgac aacttcaaga atatcaatga tacgagtggc
    13621 cacgcgagcg gcgatttcta tctgaccgtc gtcgctgaga gactcagcac ctgcgtcaac
    13681 gaaggcgaca ccgtggcgcg ctggggtggc gacgagtttg tggttattgc agagaacctg
    13741 accggttctt tgtctgaggc tgcccggcgg gccgaactca tgggcgaaaa aatgatcgcg
    13801 gcgctcgctg agccctgcga gaacccttac gcacgtgggc aatactttca gaacacggtc
    13861 agtgtcgggt tgacgctgct ctacgggcac atccattcgc ccgaagagct gctcaaacgc
    13921 gccgatattg cgatgtacca ggcgaaaatg tcgggcaaaa accgcctgcg caatttcgac
    13981 gtgcgtatgc agcgcgaaat cgagcagaaa cttgcgttcg agaatgacct gcgccgcgct
    14041 gtggccgcgg gtgagtttca gttgtattac cagccgcagg tagacgccgc gcgcgttatc
    14101 tttggtgccg aagtactgct gcgctggttt caccccacgc gcggccaggt cttgcccggc
    14161 gatttcattg cgctcgctga agagaccggc gacattttgc cgattggccg ctgggtgatc
    14221 gaagccgcct gtaagaagtt ggcacaatgg cagcgtgacg aaagaacgaa acatctggtc
    14281 ctgtcgctca atgtcagccc gcggcaattc cgcgacccgc agtttgtcga ctacgtgacg
    14341 caggcagtcc gcgatacggg aatcaatccc cggggtctga aactcgagtt gaccgaaggc
    14401 ctgatgctcg ataacataga agaaacaatc gggaaaatgg aagcactcgg caaaaccggg
    14461 ctcagtttct gcctggacga ttttggtaca ggctattcgt cgctttcgta cctcaagcgg
    14521 ctgcctttaa gccagatcaa gatagaccag tcgtttgtcc gcgacattga agtcgacgca
    14581 aacgatgctg ttttgatccg cacaattatc ggtatggcaa ataacctgaa cctcggcgtg
    14641 atcgccgagg gtgtcgagac ggaagagcag tggcagatgc tgcgcgccat gggttgcagc
    14701 gcataccagg gcttctattt tggcaggcct gcgcccgcgg cagaattgga agccctcgcc
    14761 gcgaagccgt gaagcagctt acttcttcag aacagtctca ttgcccagca ggtcgatggc
    14821 gtgaatctcg tagccagcgc cggcaggggc cctgacggtt tcgtttgcct tccactgctt
    14881 gaagtacatg tcctgcacca gcgttccgtt ctggtagatg cgcaccacga tgggaaaatg
    14941 ttcgtcggta aaagtgaagc cggccgagcc gtcttcgccg gcacgctctt caatcgccgg
    15001 gcctcgcgag tcaatcagca tacggcgaat ttgcggcggt tcaacgctgc ccgcgacgtt
    15061 ggttgcacgg tactcgatat tgtagtaccc gtaatgcccc tgtgcgaaag aggagacttc
    15121 gacatcgtct tcgtatttct gccatgcgcc gggtgtagcg ccttcagcga aaacccggta
    15181 gtcgatacgg tcgattgccg caccggcaaa ttgtgagctc agcgaaattt tggttttgcc
    15241 gccgacgatg aaaccaccgc ggccgtgcag gaaaggcgcg tagaatttta cgtaggtacg
    15301 cggtacatcg gtgtataccc agaacaccca ggtctgcaac ggcttgttct gcgagttatc
    15361 atcgctgtta taaaggttca tgcggtactg gccggcgcgg gtaaagggca caggaccgtc
    15421 gtagcgacgc cgtccattgg tattttccgg cgcgtctctt tccgtcaggc attccacatc
    15481 ataccagagt ggggtggtct cgtcgaggcg ttctgccgcc acagattttc cacggagcac
    15541 ccatttctct ttaccgtctt tgtcgataac ctgtgcgaaa acatcctgcg gttctaacgg
    15601 ctttgcctct tcgctgtgtt tcgtcgaact cttctttttt gcaggcttca cggcagctgc
    15661 cgtatcctta ccctgttggt ttcctgtggc tggttctgtg ccagttgctt tgccggcccc
    15721 ggtgtcaggt tttttcttgt ccttttttgc cacgcgtgtc gggcgctgga tttccacaac
    15781 ttcgccccag gggccgtaaa gcccgtcgcg gtacgcagaa cgcagtcgaa aataacgaaa
    15841 gcgcagcggc aggttgcgcc tgatttcggt gcgctttatc ttctcacgca ggctgctgcc
    15901 ggtgggagct tcttcttcgc cgctgatttc attgccttcg agccattcga cctggtagaa
    15961 acgcgcatcg ggccgcggtt ccagaataat ccgtaccggg taagtgtcgt catcggctgt
    16021 attgtcctgt gcataaacga gtgcgccgag tgtcagcgca gccagcaatg ccgcggccag
    16081 cctgtaagaa gccttcatgg cgtgcatgca gccgctgatg acctcttgtc gtcaaggaat
    16141 actgccagct gcgcgacgca aagagctggg ggcgaaatag cctttggacg gggtaaccag
    16201 ctacttcagc atgtttgcga aggcgtcaaa acctttgccg agagtgacgc gcacgcgggt
    16261 attgcggtcg ggcctggtac cttccagcgc cagcatcgcg attagctggt tgttatcggt
    16321 ttcgagcaaa agttcgcccg agtcggtgac ttcaacgagc cggaactcga ggttgttctc
    16381 ggtggcggcc aggtcgaccg gtgcgatcgt aagctggtct gccaggtcgc tgccctctgg
    16441 cttcagcagg aattccgtca tgaagagttc attgcagtag accttgagtt tgtagattgt
    16501 gccttttttt atgcgctcac tttctgcaga agccctgagt tcgatttcca ctccatcggc
    16561 cagctgtggc aggtcgcgga ccttctttaa gaagatgatt ttgccaggta ctgtcgaatt
    16621 acgggtctct gctgcgggtt tgatgcgcgt gtagagatcg gaggcgacaa ggcctgcaag
    16681 ctcgaagcgt gacttgtcgc tgagttcgac gccggtaacg cgcagtgcgc cgttttccca
    16741 gagcaggtcg acgcgtttca gcagggcggc tgaaaggccc atgtaagtgg gagctccctt
    16801 tattaaaaca gcaagttcag cgttgagagt cagtttacgc tgaatttttt cctggttttc
    16861 aaactgcagc tcgaccagga attcctgtgc ctgtacgcta gaggccgcaa acgaaacgcg
    16921 gttgatcttt gcctgctgtt tcggcgcctt ggccgtagcg ctcgcgtcgt cgcctgcctg
    16981 cgggctgccg gtcagaatgc gcgcaattgc cttggtgtga aacgcctggg tgacttccgc
    17041 gctgcccgtt gccttcccgt tgcggtagaa gtagattttt tttcccagcg caaagacacc
    17101 cgccttgctc atcgtgacgg tgacctggtt gcgctgcagc gcaacgtcac ggactttgcc
    17161 cgaggccgtg gcggcagttg cgcagacgaa tgataataag gaagaaagta tcaggctcct
    17221 gaaaaaggag ccaggtatgg cttgcttcag agcgcgcatt taccctcgag gcattcgcgg
    17281 gcaaactctg cagctgttgt accggcgtac tggtcggtca gcatgcggaa aatattattc
    17341 gccttttctg catcgccttc ggcctggtag ccgagcgcca tgccgacata agcttcgctg
    17401 cgaatttccg catcgggctt gcgcgcgatc acgttttcca ggattttgcg gccctcggcg
    17461 actttcttga gccgcagctg tgtcacgtag ccctcgcggt acatcgcgta cgaggccata
    17521 cggtggcgcg ggtacttgtt gtagaactgc gagtagagtg agttggcttc gccgaaattg
    17581 ccggcgcttt cggctgccga accattgcga aagagcgaga ggtcgatatc aggcagctgg
    17641 atttttccca gtttgagcgt ttcgaccggc tttatcgtaa tcgtcttgct cagcgtgttg
    17701 cggtcttcgt gcctgaagac gatggtgtac tcgccggctg gaatgtcttc gaccagcagc
    17761 ggcgacttgc actgtatctg ctcgggcgcg cgcgcggggt ctttaaactc aaaagaaagc
    17821 attgcatcag caggatcgca gccggcaatc tcgagtttgc cgagcacacg catcaggtca
    17881 ttctccgtat agtccgctac tttatcaaga gccttctgcg gattttcatc ggcgcgccat
    17941 tcaatgatgt cgctgatgac tgcgacgcct gttttcctgc tgatgacctg gtacgcaaga
    18001 atgcgagctt catcctgaac tttgatggtc gctgtcacaa aatgcgtgta gtcgccgcca
    18061 acaggtgcca tggcgccggg cgcgaagccc gagagcacga gcattttctc ggcctcaacg
    18121 atgtcgtcgc ttttggtttc gaccaggtcg aacgaagcct tcttgctgag cgcaccgagc
    18181 aacatggaat ccaggccggc aagctccggt agtttttgtg cagcctcgtt cgcggcgcgc
    18241 actggctgca ctgccagtcg ccagcggtct gaagcgccgc catcgttctg cgcaatgccg
    18301 ctcatctttt tgcgcagttc ggctgccgcg gaggtcatgc tcttctcgag gttgtcgccg
    18361 ttgccaacgg caatcgcagc aattttctgg tcattggtcc tgactgcgcg cagaattacc
    18421 tgcttttgtg cggacagcga tatggcttca ccggttatat agacatcgag agtttttgcg
    18481 gagccaccca ggtcggggca tgccttgtag cccgccgcgc atttctcctg ttgctcgatc
    18541 agtgtcttga tcgagaggcg gtccctgagg gttaaggatt tttcagcctg tatctcgctc
    18601 tgcgcgagat cggcgagctt gccgccttcg ctgccgacat cgccccggat gtcaaacacc
    18661 ccaaccgtga gcgaatggat gaatgcggtc gggacaagta atgaaaatac caaaaacaga
    18721 ttgtagcgca tgttcaacca gagaatctat ctacaggact gcaaggattt tcagaaaatg
    18781 acgccgttgc aggctgtgaa ttaggcgggc cacgttgctt ttggcaagaa actccacccc
    18841 taaaaaaagc ttgccctctc tgctgagctc atagtacagc cggccatggt aactctcgaa
    18901 cagctggagc aactcgaagg cagaattgtc aaagctctgg aacttatttc tgacctgcgg
    18961 gtcgaaaaca gccacctcga atctgaggcc gagcgcctga aagcaagtaa tgaccagctg
    19021 cgcctgacgg cagaagagcg tgaacgcgag gcgcagaagt ggcagcagca gttgcaggaa
    19081 gcgacgcagg aactcagccg cctgaaatca aaagagcagg agctcgaaac ccgtattctc
    19141 tcgattatcg caaaactgga taacctcaag ggcgcagaca aacctgcgcg gagcgagagt
    19201 gttgtatcag ctactcccgc caacgccccg gctccagccg ttgaacccgt gaaagccgag
    19261 tcgtcggcag cgccggttgc cgcagctgct gcagtgggcg cgggtaccgg tgcagcagcc
    19321 gtagctgcta atgctacact gaacgagaaa gacgagattg tcatcgacga agaagacgat
    19381 gaaatcatcc tgctcgaaga cgaagaagaa ttcgtttctg aaaaaaagac agaagagaaa
    19441 aaagaagcta aggctcctgc agatgaaatc atcatcgaag acgatgacgt tctcggtgta
    19501 tttgacgacg atgatgatga agatttcctg atcgtcgaag aagaaatgtc tgaccgtaag
    19561 aagtaaggta gtatccttga tgaagtctct tattctactt ctggtagctg catttttttc
    19621 tggcggcctg tttgctgaat cgcgctcgaa cctttttcat ataaagcgca acaagaaccg
    19681 taacgaggtg cactatgcag tccgttacga tgaaaacacc tgcaaaccga tcgagaatga
    19741 gccgctctac ggctactggc tgatgctcga aaaaggtccc aacgtatttg agccgatcgg
    19801 gcgccttgaa agaatggctt atggcattca gtcgcaggaa atcaagggca acgacctggt
    19861 agtacagctc aaggcatttc ccgaccgggc aattcgtatt accttcaatg ccgctggtgg
    19921 ctgcaagatc acgccctttc tgccgatcaa tggccaggaa gccgtgctga agtttatctc
    19981 ggtctttgca gaagagggcc tgataaagcc tacggtgaaa cacgtcgata tcagcggtgt
    20041 caaaggtggc acaccagtct ctgaacggat caacaagtag ttgttaggcg ctttttttgg
    20101 ttttgtctcc ggctttctcg gttccatgcc tgttacgggg ccgattgccc tgattgtttt
    20161 ccgcagcagt atgcgagggc acttctcgcg agcgttgcgc gtggttgccg gtgccgcggt
    20221 cgctgaggta atctattgcg cccttgcgac gttcggttac gtgcagattg ttgctgctta
    20281 tccattcctg gcgaagtata ttcggtacgt gggcgcatct ttcctggtgg tgctcggtat
    20341 tgtctttatg ttccagaaag ttcacctttc cgaagagtct gcaccggtat cagaaagaaa
    20401 gagcgcgggc cttgtctcgg gttttctcat tgctattctc aaccccacgc tgttcctgac
    20461 gtggggtagt gcaagctcca caattttttc atggttcgat tcgattggct tttgggatat
    20521 ggtattgttt cccatcttcg caggcctggg tatacttacc tggttcacga tactgcttga
    20581 gatattcaaa aaataccgtg aacagatcgg cgagaagatc ggcttctacg cgattcgcgg
    20641 cgctgctgtc gttatgatcg cttcaggaac ctatctgctg acgcaggcag gtaaatgaga
    20701 accggcagct gttgcctgcc attactcctt ctggcgttgc agtgtggcag tgcaccggtc
    20761 acgctgggtg acaaatcgat tgaaccggtc tacgagcagg tgctgcgcga ctcagcgatc
    20821 aagaagcgcc tgcaccaggt cgattttgat aaaaaatctt tctctatcga agatttgttc
    20881 ctgctgtcgc tcgaccgcac ggagcagctg gccattgcgg ccgaacgcgg tgtggctgcc
    20941 gatgccaaag tccgtcgcag ctacggcgcc tggttaccca gggtttccct cgttgccacg
    21001 cagtacgcac cggtaagtcc gggttttgcc gttcccggta tccgttttgc cgcgcggcag
    21061 aatatcatga ctggactcac cgagtatact ggcattgtcg gtgcgcgtct cgagcgtgtc
    21121 gccgaagagc agtcgctccg tgctgaaatg gcagcgcacc tgctcaatat tgccgacgcg
    21181 gcgctgcagt accagctgtg cctgcagctc ctgcacgaag cgcgcgaggc cgtgcgcatt
    21241 accgaatcgg ggctcgcgga aattcgccgc cgtgtcgcgc tgggccgcaa caaacgcggc
    21301 gacgcgctga agacagaagc gcggctgcgg cagaaacagg cagacttgct ggcggcagaa
    21361 g
//
LOCUS       DAOQJF010000007        21050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1140_length_21050_cov_5.116980, whole genome shotgun sequence.
ACCESSION   DAOQJF010000007 DAOQJF010000000
VERSION     DAOQJF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 21050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21050
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_21050_cov_5.116980"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLF85_00800"
     CDS             <1..245
                     /locus_tag="PLF85_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE18219.1"
                     /translation="PELRILGALITQLKSNTVLTKTILPVIQNYFPVFNTSISTGVAV
                     GESHLSRRSLLDYSSGVKQAKEYQAFVKEVLDGIKK"
     gene            229..1071
                     /locus_tag="PLF85_00805"
     CDS             229..1071
                     /locus_tag="PLF85_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE18220.1"
                     /translation="MASKSKKLAFAADIFEQDIEGIIRKIAVGEITPSADQPRTKFDD
                     ETIRSLADSIKTEGLMQPIVVTKEGTKYKIIAGERRYRAAQLAGLEEIECRILRKNPK
                     DTYRLAVIENLQRENLDPVDEARAFRKLKSEYGYQDADLAKIVGKSRNYINEILSVAD
                     LPTSWVERAKDAGIESKNLFIQFAQAVKNGSGDQFIEAFKTGSVTTVATAKQFNQARK
                     SPAATAGKKNTTTDDELSVKIDTKDGYCKVQIAFKTDAAATPEKTRQLESALKAAVRK
                     TLAH"
     gene            1427..2326
                     /locus_tag="PLF85_00810"
     CDS             1427..2326
                     /locus_tag="PLF85_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE18221.1"
                     /translation="MYLAIMSDILDSGTWAKLGPAAKALYPVLCKFSNHSFKPVWPGT
                     DILLKLTGFKTKKSLQEGKRELVEAGLIHVVPGTGRTPSYYYFRFDYPGSKVDIESHR
                     DIKIHPREIKTHPSGSESSSPEGVAGVTPNHINIHINKLTQTTNNNNPDLWSLLNEFF
                     GPDKRRGDFREQLVQEMLEKYGSLEVGEAVRIAISKGKDGDIRYLEGILKNRGTKQPG
                     EAKLGSEEFSFTNLPAELKIWQGHLTPMGKFESTWYFSASSDVSPSFVEEQFRRAGLK
                     IRVIKSRPEQESAEFSAARSAIN"
     gene            2405..3013
                     /locus_tag="PLF85_00815"
     CDS             2405..3013
                     /locus_tag="PLF85_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18222.1"
                     /translation="MPLMLYTVVFFFLLCVFGLFWYLQSGSPVRDAYVSPLFGDHEKN
                     LSGEDLSLLLGLRRIEHLADRGRWLLFISILLLIIEFFAVLGTLIENRGQMYWVNAVN
                     HGYVIGGFIYLLVSVVAVFLSVRLVSAEKSLSISNPFERLEAWEHKVVQADPEQKKRI
                     ALYLSQESGRLEGYFAFVFALVGCGLTLLLIWVAGWLLQFVR"
     gene            3023..5413
                     /locus_tag="PLF85_00820"
     CDS             3023..5413
                     /locus_tag="PLF85_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNE18223.1"
                     /translation="MDLTRSLGAAVAHVHSYTQALKPEHQGDSVGFSIERTAPHCSVR
                     IELGESSFLNFLRYDSALHVLADVSWSEDGNRFYRVHGVKHESHGDSREFSFPLILAR
                     YLLINFYQEAGPIHKCEVRKFQVGFRAQVKLKASSESDRLWTAENLIDRREDYGWASV
                     VREKNEADIITADLNGLFFVSEVQLKSVRDEYNFFPQTFQIQLSEDGGVWQTVQSEDH
                     FFAAPGSWYAWRFPSTRARYVRLQIDRHAHYRKGEYQSKILDLAIIGDTDFSLVGGNS
                     AAATRMASENVPGMVLLAGNNLAAPNRVVQSDDSRLRNASTEYRGIVQFARDNEAAQE
                     KAVQGNDSRLKAATVSSPGIVRLAKDGEVSDQAAVQGSDARLQRATQDAPGIVQLAKD
                     GESRPGVALQGSDSRLKAASTEAAGIVVLAKDGETATGKAVQGNDARLRLSSQTWPGI
                     VQLAGHGEIAGNKAVVADDPRLIEADEAKKGRVQFARKGENADLKAVQASDPRLQNAS
                     EENRGVVQFARDGVAVAGQAVQANDSRLSDARPAKPHVHSEYALAQHEYNSHTGNIVV
                     KHAARTAVPDGFSTPLDLHAPIVAENTEGVAGIFSGGIVSSAEGTGAYLVSRSATALQ
                     AVSRDQAAASLVSANSFALHIPRSLGGVKGSEKAIRAEGQVLVEGQVNVKGAPCLTVA
                     LPKAASEAFVEGDLLTIENGVVAKLRNESQPFIGVVAKEASVQLEGGPVGIRVAVAGL
                     VSLRVFGVVKAGDKLTLNPGQAGTCRIAQAQDRVLAVAVESVNNDREKQVLSIFAQ"
     gene            5425..6048
                     /gene="mtnB"
                     /locus_tag="PLF85_00825"
     CDS             5425..6048
                     /gene="mtnB"
                     /locus_tag="PLF85_00825"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HNE18224.1"
                     /translation="MANHSLSRELASCGNLFHRWQWSLSSSSNYSALLDPETVIISRS
                     GVDKERMTSADFMLVDLQGQPASEYASFKPSAETELHCHAYRIARQQGLPVGSVLHTH
                     SKYAVYFSRRCIKQGSIVFSGFEMQKIFEGITTHDSTVVLPVFRNSQDMSDIVADFDK
                     WLQINAWPPGYLIEGHGIYAWGKTVLHAKQKLEALEYLMDLKYMEER"
     gene            6048..6587
                     /locus_tag="PLF85_00830"
     CDS             6048..6587
                     /locus_tag="PLF85_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HNE18225.1"
                     /translation="MQSRLTISPENNPDQKEVYTDFDAIVAKLREVGIGYERWQAEKP
                     LSETASPEEVAVAYKSSIDRIMQQGYKTFDVVSLHPAAPNASEARAKFINEHTHAEDE
                     VRFFVDGSGMFYIHKGGKVFMMLCTRGDFINLPAGTTHWFDMGPKPFFKAIRVFNNRD
                     GWVGHFTGDTISQRFPKYE"
     gene            6588..7295
                     /gene="mtnC"
                     /locus_tag="PLF85_00835"
     CDS             6588..7295
                     /gene="mtnC"
                     /locus_tag="PLF85_00835"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HNE18226.1"
                     /translation="MKISGVICDIEGTTSSISFVHKVLFPLSLERMDAFLQEHGSNHE
                     VLAQLRILGQKLYGEAVQDQDLATRLAPVLKEYIRTDVKDTTLKWLQGKIWKEAFEAG
                     VVKGHVYPEVPAMLRGWRSAGLSLFIYSSGSVEAQQLMFRHSEAGDLTPLFEGYFDTT
                     TGGKRESSSYAAIASHTGILPGELLFLSDVVEELNAAESAGLLTCLLLREGVAAPAGY
                     TGKSATDFTEVQRLFFA"
     gene            7347..8831
                     /gene="glpK"
                     /locus_tag="PLF85_00840"
     CDS             7347..8831
                     /gene="glpK"
                     /locus_tag="PLF85_00840"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNE18227.1"
                     /translation="MAKPFIITIDQGTTGSRVFLISERGEVLSSAYEEFTQHFPRPGW
                     VEHDADEIWTSVERLLGKALDSAGLKATDAAAIGITNQRETTVVWEKSTGKPIHPAIV
                     WQCRRTADYCEQLKKEGRAELFRQKTGLVLDAYFSGTKIKYILDTVSGARSRAQNGEL
                     LFGTIDSFLLNRLTGEHKTDFTNASRTLLFNIHERQWDKELCEVLQVPEKMLPAAQAS
                     SSLFGKTRSVKGLPDGIPVFSLIGDQQSALYGQLCHNPGEAKNTYGTGCFVVMNLGDK
                     FLQSKNGLITTLACDAWGKPVYALEGSIFIGGAVIQWLRDKMHFFDDSGKSESLAKSA
                     AEESEVVFVPAFAGLGAPHWDQNARGAIMGLTRDTTAEQITQAALKAIALQSYDVIAA
                     MQIDSGLKLPMLKVDGGATRNHYLMEFQAGILGAPVVAPRNAETTVLGAAYLAGLGAG
                     IWKSPDELKTLNPPAATYHPVMTDAKRERELKLWKEGVSRVLTR"
     gene            complement(8837..9310)
                     /locus_tag="PLF85_00845"
     CDS             complement(8837..9310)
                     /locus_tag="PLF85_00845"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNE18228.1"
                     /translation="MLETGKKIPASLTGILVGDGEPKKTKLSELYKDKTLVLYFYPKD
                     NTPGCTTEAVCFRDNSAALKKAGLLVVGCSRDNEKAHKKFIADHDLNFPLLYDDTGKI
                     TEAFGVWAEKSMYGKKYMGILRSTFVIEKSGKIAHVFEKVSVKTHALDILKAIKG"
     gene            complement(9310..9525)
                     /locus_tag="PLF85_00850"
     CDS             complement(9310..9525)
                     /locus_tag="PLF85_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18229.1"
                     /translation="MADKHLVACEQEHEMATVLKYFKKGTSKENVDKMQNACRAWKKD
                     SGAKYKPKNRDNFYKYLEEKGILRKLG"
     gene            complement(9616..10206)
                     /locus_tag="PLF85_00855"
     CDS             complement(9616..10206)
                     /locus_tag="PLF85_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18230.1"
                     /translation="MQKVNPLRLLVSAFEPFGDRRRNTSADLLRKVRLSSLFPLDQVR
                     YHKILLPVNFDTAWPRLLCRLEAWKPDLVILTGEGKSNPVTLETAARNQRRGELENAA
                     IDPALPFVRHSAAAEQLSAALRSIQGISIENDPGDYLCNYTYFQCLSHQPEKPVLFLH
                     VHPLPEDDEEAELVRIKNLYERAIQTAIELQSRTAS"
     gene            10282..11256
                     /locus_tag="PLF85_00860"
     CDS             10282..11256
                     /locus_tag="PLF85_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNE18231.1"
                     /translation="MKKYLVYLVAAAAVINCGPKIDYNEVRNRAKSAIGTLPDKMPGS
                     EKDTPELIALGKMLYNERRLSVNDTQSCASCHMLEGKKGGVDNTKFSPGAKGALGGRN
                     APTTLNAGFHFAQFWDGRAATLSDQAKGPVLNPVEMGMPSEKAVVEKISKIEEYKAAF
                     AKAFPGSKSPITYQNIADAIAAFERTLITHDRFDDFLKGNDKALTKEEILGLQTFMNQ
                     GCTTCHVGPLLGGNSYRKMGQARPYENTKDLGRQEVTKKEEDKFLFKVPSLRNVALTE
                     PYFHDGGAATLTEAVAKMAYLQLGKQLSEQDVASIVTFLKSLTDKDRL"
     gene            11326..12174
                     /locus_tag="PLF85_00865"
     CDS             11326..12174
                     /locus_tag="PLF85_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18232.1"
                     /translation="MSAGYFISPGRVQQARRSTRPPWWEELATFLLYGLLILSANALF
                     AAPSVNVDKDYRDALNLYERRDIVSRQKAIQILERNLEVNAEHLDTQALIAFAYAHEA
                     HLMQQIGEHSTDYLASAEAFAKAVLAKQPTNTFAKKTVIFLHLVGGLQLDASRLMAKE
                     MTDKETDADMWYMQALLTEGEKSKRALTRALELKPNHVWIYSDMAFRAIQLNDLPLAE
                     KWIAALEARTPGIADLDLLKAVVAAQKKNRKDIQLHWSAFTAKAPNSPIMSRLMDKML
                     KGQSSP"
     gene            complement(12166..12561)
                     /locus_tag="PLF85_00870"
     CDS             complement(12166..12561)
                     /locus_tag="PLF85_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18233.1"
                     /translation="MPAPLPRGISLCYGRNYFAELKNGAGMQSALDRRRKRIILRRNS
                     EALFRHELAHLYLDVSWKVLPYSASEPLAQALAFSGACILQKNRTLGDDTLRHRWLIR
                     ASLAPCEAQQLFADILSAAPGVREALPLR"
     gene            complement(12656..12874)
                     /locus_tag="PLF85_00875"
     CDS             complement(12656..12874)
                     /locus_tag="PLF85_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18234.1"
                     /translation="MAEKIRLKVKCHACGFVMEGTAKYGKGNYHPEGLDFQFTAVGKL
                     VDKGGNSRVKGEVTITCPNCETRNQFDI"
     gene            complement(12883..13818)
                     /locus_tag="PLF85_00880"
     CDS             complement(12883..13818)
                     /locus_tag="PLF85_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18235.1"
                     /translation="MLTKSEKAKTSAAEWLQFWDTIRQPDRITILIGFSVLMFFVLFL
                     GYFGTRSLVRSFGFYRMSFHAPFVEDIRPGTKIRYQGALEIGEVEYLASGISGHTITA
                     KIRDDFPIPTFGSRASLSSWGYFGAKFINIEIVPTTVTELYHAQGKSMPLEKIINSSI
                     MFQQYYDAIKSEGGNLSILEKRLQQLRTTISEVKRIPYVQRHAARTVVRNATGLMNNF
                     FGVVNTTSQDLYTMLSRVNDVSEYLVYDLNKNLPKIKANMAKLEQSVAYDSESFSARL
                     LHEETIYLLLLDYARYANEKLNDMASSPYRIFFNN"
     gene            complement(13778..14626)
                     /locus_tag="PLF85_00885"
     CDS             complement(13778..14626)
                     /locus_tag="PLF85_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE18236.1"
                     /translation="MRLRKFKVAVNLNMVLLLFTAASPALADAKKRSHLLVALGSNGY
                     TIISQSNTGIEAPVGSLLKPFAAWYLLEKGQKPDSSVFCPPERKRHEGLRCWTPQGHG
                     RVDLAAALVQSCNYYFLSLFSGRDLADYQAWLTSHFDWPANLPIRRPVNVYGFDLDSG
                     IEAERLVKMYQALLQAADSGSSHAVAVRDALAGICRGTLSDFCRKMQEQPDFRLLIGK
                     TGTVQSGKKPYGIALLVLEHLSSKEKILLICYEREKTGSVVAMAAPAILSRYADKIRK
                     SKNLRR"
     gene            14634..15317
                     /locus_tag="PLF85_00890"
     CDS             14634..15317
                     /locus_tag="PLF85_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE18237.1"
                     /translation="MEGTLVRAEREALTLKVNEIFHSIQGEGAYALHPCIFIRLSGCN
                     LRCVWCDTTYSFHEGNPQSLAEILAHIAPWQSQLVEITGGEPLLQRNVYSLMELLHES
                     GRKVLLETSGSILIDKVPEFVHIILDMKAPASGESEANKYDNLALLKPSDDLKIVVAN
                     HADFSFAVDLVSANALTGRLVRPVIVQPVFGELAPAELGEWIKGSKIPFRLGLQLHKY
                     IYDPSLRAV"
     gene            15336..15689
                     /gene="queD"
                     /locus_tag="PLF85_00895"
     CDS             15336..15689
                     /gene="queD"
                     /locus_tag="PLF85_00895"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNE18238.1"
                     /translation="MVIEKEFVFDAAHFLPLVALDHKCRRMHGHTYKVVVGVEGEAGP
                     QGWIMDFADISAVIKPILKQVDHTLLNAVPGLENPTAENIAQWLYEKSRKQIPAVSYV
                     IVQEGVASRVIYPRR"
     gene            15686..16402
                     /gene="queC"
                     /locus_tag="PLF85_00900"
     CDS             15686..16402
                     /gene="queC"
                     /locus_tag="PLF85_00900"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNE18239.1"
                     /translation="MTREKAVVLLSGGLDSATCVYKAKGQGYDPIALSFDYGQRHKAE
                     LQAAKRIAVHLGIEHRVITVDLRKIGGSALTSDIAVPKGGEHLKAEGLIPVTYVPVRN
                     LIFLSLAYALSEASGASAIYIGVNAIDYSGYPDCRPDFIESFSRTAALASKTGREGHG
                     ADIVTPLISLSKAEIAAEAVRLGVPVELTWSCYDPQASGTSLVPCRNCDACILRRDGF
                     MAAGLALSPEEIYGSPTERA"
     gene            complement(16359..16949)
                     /locus_tag="PLF85_00905"
     CDS             complement(16359..16949)
                     /locus_tag="PLF85_00905"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNE18240.1"
                     /translation="MFSALTKTPLLLASSSPRRREILERLGFTCEIRPAGIDEESIRH
                     ADAAEQTLLLAEKKADVVALPGRLTVAADTVVVLDGEVIEKPRDKGHARSMLQSLSGR
                     DHIVYTAVAVVFPKGERLRFIEETRVFFDALSTETIESYIASPQPYDKAGGYGAQDAF
                     GMACIRRFEGCYFNVMGFPASRFMRVLSENRKFLQG"
     gene            17002..17382
                     /locus_tag="PLF85_00910"
     CDS             17002..17382
                     /locus_tag="PLF85_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNE18241.1"
                     /translation="MGYLVAAGMSGFFAVFFGAFGSHVLRASLSEKAFSVFQTANAYH
                     FWHTLALLAVALLRIARPQKFFTSSAIALLAGLFLFSGSLYLYAIYQIPVFARLAPLG
                     GSAYMLGWLFLVLGSVRAFARSSE"
     gene            complement(17608..18831)
                     /locus_tag="PLF85_00915"
     CDS             complement(17608..18831)
                     /locus_tag="PLF85_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE18242.1"
                     /translation="MSKAGFFTVYLVVFLDMLGFGVMIPAIRDLTTALVKNSGLSWPS
                     PEIFMGLLMAAYSGSQMISAPILGRLSDIYGRKPVFLVSAVGNLLSYVVWILSSSYWP
                     FLLGRLLSGITGGNIAIAQSILADNTAPAERPRAMGLLGACIGMGFVLGPFIGGLMVN
                     INHEFAININPFWQIGVVPLLLASVSVILIIFNHFGMAHESTEKGGVLAGLRSVFRPP
                     QSSTYATQLLSQLSFVSFEVLFAWILQRQYGFNLKETYYFFGFQGILLALVQGGIYRR
                     VEKRRPPADWVQLGLLGSAVGMAVLPWIGGLELGQVFGMAGKTVLLSVVLVLMSLALG
                     FGTPSLNAFASLNAPKNEQGQTMGNMQGLAAMARFVAPVAATGLYAVWLPLPFVLGGL
                     LCLAAWLVFSRGRSA"
     gene            18926..19423
                     /locus_tag="PLF85_00920"
     CDS             18926..19423
                     /locus_tag="PLF85_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18243.1"
                     /translation="MKTPSDLRLLALAILQQSPTEPKTRYQVMIEINQIISEILPPYS
                     PGAVYHELKLLKRSNMIDIDHTSRVVITEPGLNLLHQSLLEAPTPRPLLSLLVRVLTL
                     LLLNNKTQKEKGLHKLQVEMIKFNQEVAVNERWSSDDKLSLITKLLQLGLSMRKTISA
                     FVAQL"
     gene            complement(19466..20320)
                     /locus_tag="PLF85_00925"
     CDS             complement(19466..20320)
                     /locus_tag="PLF85_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNE18244.1"
                     /translation="MEQWAHEMRFVAIDLETTGLNPYKHEIIEIGAVSFTLDGLDKDF
                     SILIKPERKMDPKARAIHQISAEELATNAVSLEEGVSAFMQFMTDGSWVFHNAPFDLA
                     FLKQAYGSLKRPWPNRSYYDHLQLSRKLRAERMSHSLESIKKELGIETGQAHRALADA
                     RATALAFTHLLLTEPDIFSSKTKFQRIRRWQRKTEKYEILFPKNFDQIEHYFLGKIRQ
                     EAILKVDFFDRNGLPQRALVQPLEVMIFSQNVYVRSKIHPAGLELLVPLNRATIHDQE
                     LGAISFAP"
     gene            20361..20675
                     /locus_tag="PLF85_00930"
     CDS             20361..20675
                     /locus_tag="PLF85_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18245.1"
                     /translation="MLYRQLVFFGHGRPYGMASRVRQGGAIACCFSALSKAARLAIYV
                     AFAVFALLCHAACRVRKPTRSSLGDTGRIKAAYLHREGHSTFIVRRTKNFPIALAEYL
                     WV"
BASE COUNT         5116 a         5383 c         5606 g         4945 t
ORIGIN      
        1 atccagagct ccgcattctg ggcgccctca ttacacaact gaagagcaac acggtcctga
       61 caaaaactat cttgccggtg atccagaatt attttcccgt gttcaacacc tcgatctcga
      121 ctggggtggc agttggcgag agccacctgt cgcgccgttc gctgctggac tacagctctg
      181 gcgtcaagca ggccaaagaa taccaggcct ttgtcaaaga agtgctcgat ggcatcaaaa
      241 agtaaaaaac tggccttcgc tgctgatatt ttcgagcagg atatcgaagg tatcatccga
      301 aagattgccg ttggtgaaat taccccttcg gccgaccagc cgagaaccaa gttcgacgac
      361 gaaaccatac gcagcctggc tgactccatc aagactgagg gcttgatgca gccgatcgta
      421 gttactaaag aaggaactaa atacaagatc attgcgggcg aacggcggta ccgggctgcg
      481 caactagccg gcctcgaaga aattgagtgc cggatactgc gcaaaaaccc aaaagacacc
      541 taccgactgg cagttatcga gaacctgcag cgcgaaaacc ttgacccggt agatgaggca
      601 agggctttcc gcaagctcaa aagcgaatac ggctaccagg acgcagacct ggctaagatc
      661 gtaggcaaat cacgaaatta catcaacgaa atcctgtccg tagccgacct gcccacaagc
      721 tgggttgaaa gagccaaaga cgcaggcatt gagtcgaaaa acctgttcat ccagttcgcg
      781 caggctgtaa aaaacggcag cggggaccag tttatcgagg catttaaaac cgggtcggta
      841 acgaccgttg caacggcaaa acagttcaac caggcacgca aaagcccagc tgcgacagcc
      901 ggtaaaaaaa atacaacaac tgacgacgag ctctctgtaa aaatcgatac caaagatggc
      961 tactgcaagg tgcagatcgc attcaagact gacgccgcag caacaccaga gaagactcga
     1021 caactcgaaa gcgccttgaa ggctgcagtc cgcaagacac tggcccactg aaacccaagg
     1081 ccaacctctg cccgagagaa aacaacggca acagcggaaa aagattgaca gttcagaatt
     1141 atgtcacatt gttagagtga agacaagggc ttgttggatt cttgcgccaa ataaaaaagt
     1201 agggcgctcc ctcgttttct gaaagaaaga cccctcgagc aaggcggagc tttaaccgcg
     1261 tggcaagggg ctggttgacc cctttggttt gggcaaaaaa aagggcatgt tgccatgccc
     1321 gaaattccaa agggaacttg ttggatgatg agacataatc agctgccaac aatccgtcaa
     1381 ccagaaattg attatttttg agcgagggag aatggataca aataacatgt acctggctat
     1441 catgtccgac attttggaca gtggcacttg ggcaaagctt ggcccagcag ccaaggccct
     1501 ctatccggtc ttgtgtaaat tcagcaacca cagctttaag cccgtttggc ccgggacgga
     1561 tatcctcttg aaactcactg gcttcaagac caaaaaatcc ctacaagaag gcaaacggga
     1621 gctggtcgaa gcaggtttga ttcatgtcgt acccggcaca ggcagaacac cctcgtatta
     1681 ttatttccgg tttgattacc cgggcagcaa ggtggatatc gagagccacc gggatataaa
     1741 aatacacccc agagagataa aaacacaccc ctcagggagt gaatccagta gcccggaggg
     1801 ggtggcgggc gtaactccga accacatcaa tatccacata aacaaactta cacaaactac
     1861 aaacaacaac aaccctgact tgtggtcgtt gcttaatgaa ttctttgggc ctgataaacg
     1921 acgaggagac tttcgcgaac agctcgttca ggagatgctc gaaaagtatg gctcgctcga
     1981 ggttggcgag gcggtgcgca ttgcaattag caaaggcaaa gacggcgata tccggtacct
     2041 tgaaggtata ttgaaaaacc gggggacaaa gcagcccggt gaagctaagc tgggcagcga
     2101 agagttttca tttaccaact tgcctgccga gctaaaaatt tggcagggac accttacccc
     2161 aatggggaaa tttgaatcga cctggtattt ttctgccagt agcgatgtct cgccgagttt
     2221 tgtggaagag caattccgga gggctggcct gaagatccgg gtgataaaat ccaggccaga
     2281 gcaggagtct gctgaatttt cggcggcgag atcggcaata aattgattga aacactattc
     2341 gacctttaat gatggggcga aaatcaacta gccgttcgcg gcagagactt ttacgggagg
     2401 ctttatgcca ttgatgctct atactgtagt ctttttcttt ttactttgtg tattcggttt
     2461 gttttggtat ttgcagagtg gttcgccagt tagggatgct tacgtttctc ctctatttgg
     2521 tgatcacgaa aagaacctca gcggcgaaga tctgtcgctg ttgctcggtc tgcgccgcat
     2581 tgagcatctg gcagaccgcg ggcgctggct cctctttatt tctattttgc tcctgataat
     2641 cgaatttttc gccgtactcg gtacgctgat tgaaaaccgc ggccagatgt actgggtgaa
     2701 tgcagttaat cacggttacg tgattggcgg atttatctac ctgttggttt cagtggttgc
     2761 cgtatttttg tcggttcgtc ttgtttcagc agaaaagtct ctctcgattt caaacccttt
     2821 cgaacggctc gaagcctggg agcacaaggt tgttcaggca gatcctgagc agaaaaaacg
     2881 catagcgctc tacctgagcc aggagtcagg ccgactggaa ggttatttcg cgtttgtttt
     2941 tgccctcgtt ggctgtggtt tgacattgct cttgatctgg gttgctggct ggctgctgca
     3001 attcgttcgc tgaagttact gaatggatct cacgcggtct cttggtgcag cagttgccca
     3061 tgttcattct tatacccagg ccctgaagcc ggagcaccag ggcgattccg ttggtttttc
     3121 gatagaaaga actgcgccgc actgcagcgt tcgcatcgag ttaggtgaaa gctccttcct
     3181 gaattttctg cgctacgata gtgcgctgca tgttctcgca gatgtttcgt ggtcagagga
     3241 cggaaaccgg ttctaccgcg tgcatggtgt aaagcatgag agtcacggcg actcccgtga
     3301 gttttccttc cctttgatcc tggctcgtta cctgctgata aatttttacc aggaagctgg
     3361 cccaattcac aagtgtgagg tccgtaagtt ccaggtggga tttagggcac aggtaaaact
     3421 taaagccagt tcggagtccg accgactctg gacggcggaa aacctcatcg atcgccgtga
     3481 agattacggc tgggcttcag tcgttcgcga aaaaaatgaa gcggatataa taacagccga
     3541 tctgaacgga ctgttcttcg tttctgaggt gcagctgaaa tcggtgcgtg atgaatataa
     3601 ctttttccca cagacctttc agattcaact ctccgaagat ggtggtgtgt ggcagacggt
     3661 acagtctgaa gatcattttt tcgctgcgcc gggtagctgg tatgcatggc gatttccttc
     3721 gacgcgtgcg cgttatgttc gcctgcagat cgacaggcac gcgcactacc gtaaaggtga
     3781 ataccagtca aagattctcg atcttgccat aattggcgat acagactttt ctcttgtggg
     3841 tggtaattct gctgcggcaa cgcgaatggc cagcgaaaac gttccgggaa tggttttact
     3901 tgccgggaac aaccttgctg caccgaatcg ggtagtgcag agtgacgatt cgcgtttgcg
     3961 aaatgccagc acagaatatc gcgggatcgt ccagtttgcc cgcgataatg aggccgcaca
     4021 ggaaaaggcg gtgcaaggaa atgactcacg cctaaaagct gcgacggtat cttcacctgg
     4081 tattgtgcgt ctcgcaaagg atggcgaggt gagcgaccaa gctgctgtgc aaggtagcga
     4141 tgcgcgctta cagcgtgcga cacaggatgc cccaggtatt gtgcagttgg ctaaggacgg
     4201 agaaagccgc cccggagttg cactgcaggg gagtgacagc aggctgaagg ctgcttccac
     4261 tgaagcagca ggtattgtcg tgctggcaaa agatggcgag actgctacag gtaaggctgt
     4321 acagggtaac gatgcaagat tacggctctc cagccagaca tggccaggta ttgttcaatt
     4381 agctggccat ggagaaatag ctggcaataa agcggttgta gcagatgatc cacgattgat
     4441 tgaggccgat gaggcaaaaa aaggtcgcgt gcagttcgca cgcaagggag aaaatgctga
     4501 cctgaaagcc gtacaggcaa gcgacccccg gctgcagaat gcgagcgaag agaaccgcgg
     4561 ggtagtacaa tttgcgcgcg atggtgttgc cgttgctggc caggcggtac aggccaacga
     4621 tagccggctg agcgatgcgc gtccggcgaa gccccacgtg cacagcgagt atgcgctggc
     4681 gcagcacgaa tataattccc atacaggcaa tattgttgtt aaacatgcgg cgcgtaccgc
     4741 cgtgcctgat ggtttttcca cgccgctgga tttgcatgcc ccaattgtgg cagaaaacac
     4801 agagggcgtt gcgggtattt tttctggtgg gatcgtctcg agcgccgaag gcacaggagc
     4861 ttacctggtt tcgcgctctg cgacagcgct gcaggctgtg tctcgcgatc aggcagcagc
     4921 aagtctcgtt tcggcaaata gctttgccct tcatattccg cgctctttgg gcggagtgaa
     4981 gggttcagag aaagcgattc gtgcagaggg acaggttctt gttgaagggc aggtcaatgt
     5041 taaaggagcg ccatgcctga cagttgcctt gccgaaagct gccagcgagg cttttgtcga
     5101 gggtgacctg ctcacaatcg aaaatggcgt tgtcgcgaag ctacgcaatg aatcacaacc
     5161 gtttatcggt gtcgtagcaa aagaggcctc tgttcagctg gaaggtggtc ctgttggtat
     5221 acgggttgct gtggcaggcc tggtctcgct tcgggtattt ggcgttgtca aggctggtga
     5281 taagctaacg ctcaatcccg gccaggctgg cacctgtcgt atcgcgcagg cccaggaccg
     5341 ggttcttgca gtagccgttg aatcggtaaa taatgatcgc gaaaaacagg tcttgagtat
     5401 atttgcgcag tgacccctga tctattggcc aatcactctc tttcgcgcga actcgccagc
     5461 tgtggtaatt tgttccaccg ctggcagtgg tctctttcga gcagcagtaa ttactcagca
     5521 ctgctcgatc ccgagacggt gatcatctcc cggtcgggag tggataaaga acgcatgacc
     5581 tccgccgatt ttatgctggt cgacctgcag ggacagccag cgtcagaata cgcgtcgttc
     5641 aaaccttcgg cagagacgga gctgcactgt catgcgtatc ggatcgcccg gcagcagggc
     5701 ttgcctgtcg ggtcagtgtt acacacccat tcaaaatatg ccgtctattt ttcgcgccgg
     5761 tgcataaaac aaggatcaat cgtcttttct ggcttcgaga tgcagaaaat ttttgaaggt
     5821 ataaccacgc acgatagcac agtcgtgcta ccggtttttc gcaatagcca ggatatgtca
     5881 gatattgttg cagattttga caagtggctc cagattaacg cctggccccc gggttatctc
     5941 attgaaggtc atggcatata tgcgtggggc aaaactgttt tacacgccaa gcagaaactc
     6001 gaggcgctgg agtacctgat ggatctcaaa tacatggagg aacgataatg caaagccgac
     6061 tgacaatttc gccggagaat aatcccgacc aaaaggaagt atatacagat ttcgatgcaa
     6121 ttgttgcgaa gttgcgagaa gtcggtattg gttacgagcg ctggcaggcc gaaaaaccct
     6181 tgagtgaaac tgcttcgccg gaagaggttg ccgttgctta caagtcttct attgaccgga
     6241 tcatgcagca ggggtataaa acttttgatg tggtttctct gcatccggct gctcctaatg
     6301 cttctgaagc gcgtgcaaaa ttcattaatg agcacacgca cgcagaggac gaagtcaggt
     6361 tcttcgtcga cggctcaggc atgttctaca tccacaaggg tggaaaggtg ttcatgatgc
     6421 tctgtaccag aggagatttc atcaacctgc ctgctggcac cacgcactgg ttcgacatgg
     6481 gcccgaaacc gttcttcaag gcgatccggg tttttaataa ccgcgatggc tgggttgggc
     6541 attttaccgg cgataccatt tcgcagcgat ttcctaagta cgaataggtg aagatttcag
     6601 gggttatttg cgatatcgag gggacaacct cctcgatatc gtttgtgcac aaagtgctct
     6661 ttccgctctc cctggagcgt atggacgcct ttctgcagga gcatgggtcg aaccatgagg
     6721 ttctggcgca gctgcgcatt cttgggcaaa agttatacgg cgaagcagtg caggatcagg
     6781 atctcgcgac acggcttgca ccggtactga aagaatatat aagaacagac gttaaagata
     6841 caacgctgaa gtggctccag gggaagatct ggaaagaagc ctttgaggcc ggcgtcgtaa
     6901 aaggccatgt ttatcctgag gtgcctgcca tgttgcgcgg ctggcgctcc gctggcctta
     6961 gcctgtttat ctatagctca ggttctgtag aggcgcagca gttgatgttc cgccattcag
     7021 aggcgggcga tctgaccccc ctctttgagg ggtattttga taccaccacc ggagggaagc
     7081 gcgaaagcag ttcgtacgcc gcaatagcca gtcacactgg catattgcca ggcgagctgc
     7141 tgtttctttc ggacgtggta gaggaattga acgccgctga gtctgcgggg ctgctgacat
     7201 gtttgctgct gcgcgaagga gtggcggctc cagccggata taccggcaaa agtgcgaccg
     7261 actttaccga ggtccagcgc ctgttttttg cctgagcgcc gcgcgtaata tggttgcctt
     7321 gcggcgcttg ctgcagaaat cagcacatgg ctaaaccatt cattatcacg attgaccagg
     7381 ggacaaccgg gtcccgggtg tttctaatct ctgagcgcgg cgaagtgctt tcgagtgcat
     7441 acgaagagtt cacgcaacat ttccccaggc caggctgggt agagcacgat gccgatgaaa
     7501 tctggacaag cgtagagcgt ctgctcggta aagcgctcga tagcgcagga ctcaaggcca
     7561 cggatgcggc cgctattgga atcaccaacc agcgggaaac aacggtcgtc tgggagaagt
     7621 ctaccggcaa gcctatacat cccgcaatcg tttggcagtg caggcgtact gcagattatt
     7681 gtgagcagct caagaaagag ggcagggcgg agctgtttcg gcagaagacc ggtttggtcc
     7741 tggatgccta tttttccggc acaaagataa aatacattct tgataccgtt tctggcgccc
     7801 gctctcgtgc acagaacggt gaattattgt ttggaacaat tgatagtttt ctgctcaacc
     7861 ggcttaccgg agagcacaaa acagatttta ccaatgcttc gcggacgctg ctgttcaata
     7921 ttcatgaacg ccagtgggat aaagaacttt gcgaagttct gcaggtgcct gagaagatgc
     7981 tccctgctgc acaggcaagc agcagcctgt ttggcaagac caggagcgtg aaagggttgc
     8041 ccgatggcat tcctgtcttt tcgctcattg gcgatcagca gtcggcgctc tatggccagc
     8101 tttgccacaa tccgggcgag gccaagaata cctatggcac tggatgtttc gtcgttatga
     8161 acctgggtga caagtttctg cagagcaaga atggtttgat tacgacgctg gcgtgcgatg
     8221 cgtggggtaa gccggtctat gctctcgagg gctcaatatt catcggtggg gcggtcatac
     8281 aatggctgcg ggacaagatg catttctttg acgactcggg taaatcagag agcctcgcaa
     8341 agtctgcagc tgaagagtca gaagtggtgt tcgtgcctgc cttcgctggt ctcggagctc
     8401 cacactggga ccaaaacgca cgcggcgcca tcatggggct gactcgcgat accactgccg
     8461 agcagatcac ccaggccgca cttaaggcca ttgcgctgca gtcgtatgat gtgatcgccg
     8521 cgatgcaaat agatagcggg ctgaagctgc cgatgcttaa ggtcgatggc ggggcgacga
     8581 gaaatcatta cctgatggag tttcaggctg gcatactcgg agcaccagtg gttgctccgc
     8641 gtaacgcaga aacaaccgtg ttgggggcgg cttacctggc gggccttggt gcaggtatct
     8701 ggaaatcccc ggacgaattg aagacactga acccgcccgc ggcaacatac catccggtca
     8761 tgacggacgc aaagcgtgaa agagaactca agctctggaa agaaggtgtt tcaagagtgc
     8821 tgaccagata gactggtcag cctttaatcg ccttaagaat gtcgagcgcg tgcgtcttta
     8881 ctgagacttt ttcaaagacg tgcgcgattt tgcccgactt ttcgatgaca aaagtggagc
     8941 ggagaatgcc catgtatttt tttccgtaca tagatttttc tgcccaaaca ccgaatgcct
     9001 cggtgatttt tccggtatca tcgtagagga gcggaaaatt gagatcgtgg tcggcgataa
     9061 atttcttatg ggctttctcg ttatcacggg aacagccaac cacaagcagc cctgctttct
     9121 ttagagcggc gctgttgtcg cgaaaacaaa ctgcttcagt tgtgcagcct ggcgtgttgt
     9181 ctttcgggta aaaataaagg acaagcgtct tgtctttgta cagttcagag agtttggtct
     9241 ttttaggttc cccatcgccg accagaatgc cggtcagcga tgcggggatt ttttttccag
     9301 tctcgagcat tagccgagtt tgcggagaat gcctttctct tccaggtact tgtagaagtt
     9361 atcgcggttc tttggtttgt attttgcgcc gctgtctttc ttccatgccc ggcatgcatt
     9421 ctgcattttg tctacgtttt cttttgatgt gcctttctta aagtacttca gcaccgttgc
     9481 catttcgtgc tcttgttcac atgctaccaa gtgtttgtct gccattaaat tcccccagca
     9541 agattttcgc gagggctgca gtttgcagtg gtgccgtcaa ttgcttttat gccggtctcg
     9601 gtcttttacg taatgctacg aagctgtacg agattggagt tcaatagctg tttgtattgc
     9661 gcgttcatat agattcttga tccgaacaag ctccgcctct tcatcatctt ctggcagtgg
     9721 atgaacgtgc aggaaaagca ctggtttttc aggctggtgc gataagcatt gaaaataggt
     9781 gtagttgcac agatagtctc ctgggtcgtt ttcgattgag atcccctgaa ttgaacgcaa
     9841 tgcggctgaa agttgttctg cagctgctga atgtcgtacg aacggtagag ccgggtcgat
     9901 agcggcgttt tccagctcgc ccctgcgttg attgcgtgca gctgtttcca gtgtgactgg
     9961 gttggacttg ccttcgccgg tcaaaatgac aagatccggc ttccatgcct ccagccggca
    10021 taggaggcgg ggccacgccg tgtcaaagtt gaccggcagg agaatcttgt ggtatcgtac
    10081 ctggtcgagg ggaaacaggc tgctcaggcg gactttccgc aagaggtctg ctgaggtatt
    10141 ccggcgccgg tcgccaaagg gttcaaaagc agatacaagc aggcgcaagg ggttcacttt
    10201 ctgcattagg gagtctcaag ctgtcaatga tatgtcatca aaaagttgac gccccgcctt
    10261 gatataatta ttttaatgga tatgaaaaaa tacttagtat atctagtggc tgcggcggca
    10321 gttatcaact gcggtccgaa aatcgattac aatgaagtgc gcaaccgcgc aaaatcagct
    10381 attggcacgc ttccggacaa gatgccgggt agtgaaaaag acactcctga gcttattgca
    10441 ctgggcaaaa tgctctataa cgagcgtcgg ctttcagtga atgacacgca gtcttgcgcc
    10501 agctgccata tgcttgaggg taagaagggt ggggtagaca acacaaagtt cagcccaggt
    10561 gcaaaaggtg cccttggtgg tcggaacgca ccaactaccc tgaatgctgg tttccatttt
    10621 gctcaattct gggacggtcg cgcggctact ctctccgacc aggcaaaagg cccggtgctg
    10681 aaccccgtcg agatgggcat gccgtcggaa aaggccgttg tagaaaaaat ttcaaaaatt
    10741 gaggaataca aggctgcatt tgcaaaagct ttccccggat cgaaaagccc aatcacttac
    10801 cagaatattg ctgacgcgat cgctgctttt gagcggactt tgatcacgca cgatcgtttt
    10861 gatgactttt tgaaaggcaa tgataaggca ctgacaaaag aagaaattct gggtttacag
    10921 accttcatga atcaaggttg cacgacctgt cacgtgggtc cgttgctcgg cggcaacagc
    10981 taccgcaaga tgggccaggc tcgtccttac gagaacacga aagatctagg tcgccaggaa
    11041 gtaaccaaaa aagaagagga caagttcctc tttaaagttc cttcgctgcg caacgtggca
    11101 ctgactgagc catacttcca cgacggtgga gctgcgacac ttaccgaagc ggttgctaaa
    11161 atggcatacc tgcagctcgg caaacagctc tcagaacagg atgttgcatc cattgtaacc
    11221 ttcctcaagt ctctgacaga taaagaccgc ctctagccga aggtagcccg tgtaaaacgg
    11281 gcattctggt caaaagccga cgatatcagg tcgataataa gactgatgtc agccggctat
    11341 tttatttctc caggcagggt acaacaagcc cgccgttcaa cgcgtcctcc ctggtgggaa
    11401 gagctggcaa cgttcctgct ctatgggctg ctaattctca gcgcaaacgc tctgtttgca
    11461 gccccctcgg taaacgtcga caaggactac agggatgctt taaacctgta cgagaggcgc
    11521 gatatcgtct cacggcagaa ggcaatccag attctcgaac gaaatctcga agtcaatgcc
    11581 gagcatctcg atacgcaggc gctcatcgct ttcgcttacg cacacgaggc gcaccttatg
    11641 caacagattg gcgaacattc aacagactat cttgcttcgg ccgaagcttt tgctaaagcg
    11701 gtgcttgcca aacaaccaac taatactttt gctaaaaaga cagtcatctt tctacacctg
    11761 gtcggtggtc tgcagcttga tgcatcaaga ctcatggcca aggaaatgac cgataaagag
    11821 actgatgcgg atatgtggta tatgcaggcc cttctcaccg aaggcgaaaa atccaaacgg
    11881 gcactcacac gcgcgctgga gctgaagccc aatcatgtct ggatctactc agacatggca
    11941 ttccgtgcta tccagctaaa cgacctgccc ctggcagaga agtggatcgc tgcgcttgaa
    12001 gcgcgcaccc cgggtatcgc cgaccttgac ctgttaaagg cagtcgtggc tgcacagaag
    12061 aagaaccgca aagatattca gttgcattgg tctgctttta ccgcaaaagc tcccaattcg
    12121 ccaattatgt cccgactgat ggacaaaatg ctaaagggcc agtcttcacc gtaggggaag
    12181 agcctcgcgc acacctggtg cggcagaaag aatatcggca aaaagctgct gcgcttcgca
    12241 tggcgccagg cttgcccgga ttaaccatcg gtgcctgaga gtatcgtcac caagggttct
    12301 gtttttctgc aggatgcagg cgccagaaaa tgcaagcgct tgcgccaggg gttctgatgc
    12361 ggagtagggt agtaccttcc agctgacatc gaggtaaagg tgcgccagtt cgtggcgaaa
    12421 cagagcttcg ctgtttcgcc gtaatattat ccgctttctg cggcgatcga gagccgattg
    12481 catgcctgcc ccgttcttca gctctgcaaa gtagttgcgg ccatagcaga gcgagatgcc
    12541 gcgaggcagg ggggcgggca caaattttct cgaaaaatct ccccagatca cctgtgctcc
    12601 accgcggcgg tccaggtttt cacactgggc aaaaaccgct ggcgcaagcg ccaggctata
    12661 tatcaaactg gttgcgagtt tcacagttgg ggcaggtgat tgtgacctcg cccttgaccc
    12721 ttgagtttcc gcccttgtcg acgagtttgc ccacggcagt gaactgaaaa tcaaggcctt
    12781 cagggtggta attgccttta ccgtacttgg ctgtcccttc catgacaaat ccgcaggcat
    12841 gacacttcac tttgagtctt attttttcag ccatggctca ccttaattat tgaagaaaat
    12901 tctgtagggc gaagacgcca tgtcattcag tttttcattc gcataccgtg cgtaatcgag
    12961 caacaaaagg tagatagtct cttcatgcaa caggcgcgca ctgaagcttt cgctgtcata
    13021 ggcaacgctt tgctccagct ttgccatatt tgcctttatc ttcggcaggt ttttgttcag
    13081 gtcatagaca agatactcgc tgacatcgtt cacgcgcgac agcatggtat agagatcctg
    13141 gcttgtggta ttcaccacgc caaagaagtt attcatcagg cctgtcgcat tgcgcaccac
    13201 cgtccgcgca gcatgacgct gcacgtaggg aatacgcttc acctcggaga tagttgttct
    13261 tagttgctgc agccgtttct ccaggatgct caggttacca ccctctgatt taattgcgtc
    13321 gtagtactgc tggaacatga tcgacgaatt gatgatcttt tcgagcggca tcgatttgcc
    13381 ctgtgcatgg tagagttcag tgacggttgt cggcacaatc tcaatgttga tgaacttggc
    13441 cccaaagtat ccccaggagc tcaaagaagc cctgctgccg aatgttggta tcgggaaatc
    13501 gtctcgaatc ttagcggtaa tagtgtggcc agaaattccc gaggcaaggt attccacctc
    13561 gccaatttcg agcgccccct ggtagcggat tttcgttccc ggtcggatat cctccacaaa
    13621 aggagcgtga aacgacattc tgtagaaacc gaatgagcga acgagtgagc gcgtgccgaa
    13681 atagccgaga aaaagtacga agaacatcag cacggaaaag cctatgagta tggtgattct
    13741 gtcaggctgc ctgatagtat cccaaaactg cagccattca gcggcggagg tttttgcttt
    13801 ttctgatttt gtcagcataa cgactgagta tcgccggggc ggccattgcc acgactgagc
    13861 cagttttttc ccgttcataa cagatcaaca ggattttctc tttgctggat aaatgttcca
    13921 ggacgagcag ggcaataccg tagggcttct tgccgctctg cacggtccct gttttcccga
    13981 tcagcagacg aaagtcgggt tgctcctgca ttttccggca aaaatccgaa agggtgccgc
    14041 ggcaaatacc tgcaagcgca tcacgcacag cgaccgcgtg ggagctccca ctatctgccg
    14101 cctgcaacag cgcctggtac atcttaacga gacgctctgc ttcaatgccg ctgtcgagat
    14161 caaaaccgta tacgttcacc ggcctgcgga tcggcaggtt ggcgggccag tcaaagtggc
    14221 tggttaacca ggcctggtag tcggcgaggt cacgcccgga aaaaagtgac aggaaatagt
    14281 agttacagct ttgcacgagc gcggccgcca ggtcgaccct gccatggcct tgcggggtcc
    14341 agcagcgcag gccctcgtgg cgctttcttt cgggggggca aaagacagaa ctgtctggct
    14401 tctggccttt ctccagcaga taccacgccg cgaagggctt taggagcgac ccaaccggag
    14461 cctcaatacc tgtgttcgat tgcgaaatta tcgtataacc gttgctcccc agagccacaa
    14521 gcaggtgcga gcgctttttg gcatcagcaa gagcggggga tgctgcggtg aacagcaaca
    14581 aaaccatatt tagatttacg gcgactttaa atttgcgtaa cctcacgcgg acaatggaag
    14641 gcacactagt cagggcggaa agagaagcgc tcaccctgaa ggtgaacgaa atcttccaca
    14701 gtatccaggg cgagggcgcg tatgcgctgc atccctgtat cttcatccgt ttatcgggct
    14761 gcaatcttcg atgtgtctgg tgcgatacca cttattcctt ccatgaaggt aatccgcagt
    14821 ctttggcaga aattctcgcc cacatagcgc cctggcagtc gcaactggta gaaatcaccg
    14881 gtggagagcc gctgttacag cgcaatgtct actcactgat ggagctgctg cacgagtcgg
    14941 gcagaaaggt gctgctcgag acatcaggct cgatactcat cgacaaggtt cccgaatttg
    15001 ttcatatcat cctggacatg aaagcccccg cgtcaggtga aagcgaagcg aataaatacg
    15061 acaacctggc tttactgaag ccttctgatg atttgaaaat cgtcgtagcc aatcatgccg
    15121 atttctcttt tgccgtcgac ctagtctcgg caaatgcact taccggcagg ctagtgcgcc
    15181 cggtaatcgt gcagccagtt tttggtgagc ttgcccctgc cgaactgggc gaatggataa
    15241 aaggctcaaa gattcccttt cgcctcgggt tgcagctgca caaatatatt tacgatccca
    15301 gcctgagggc ggtctgacac gacgcgggca ctactatggt aattgaaaaa gaatttgtct
    15361 tcgacgcggc gcattttttg ccgctggtgg ctttagacca taaatgtcgt cgtatgcacg
    15421 gtcataccta caaagttgtg gtgggtgtcg aaggcgaagc aggtccgcaa ggctggatca
    15481 tggattttgc cgacatcagc gcagtcatca aacccatatt aaaacaggtt gatcacacat
    15541 tactcaatgc agtccccggg ctggagaacc ccacagcaga gaatatcgcg cagtggctct
    15601 atgaaaaaag cagaaaacaa atacctgccg tcagctacgt cattgtccag gagggtgtcg
    15661 cttcccgcgt catatatccg cgccgatgac acgcgaaaaa gccgttgtac ttctttcagg
    15721 cggtctggat tccgctacct gcgtttataa agcaaagggg caggggtacg atcctattgc
    15781 actgtcgttt gactatggcc agcgacacaa ggcagagcta caggcggcaa agcgtatcgc
    15841 agttcacctg ggtattgaac accgggttat cacagtagac ctgcgcaaaa ttggcggctc
    15901 agcgctgacg tcagacattg ccgtacccaa aggtggcgaa cacctcaaag cagagggcct
    15961 gattcctgtg acgtatgtgc ctgtccgtaa tctcattttt ctctcgcttg cctatgcgct
    16021 ctcagaagcg tcgggagcat cggccattta cattggcgtt aatgcgattg actactccgg
    16081 gtaccctgac tgccgccctg attttattga gtccttctcg cgtactgccg cgcttgcctc
    16141 taagacaggc cgcgagggcc atggcgcaga cattgtaacg ccattgattt cgctctcaaa
    16201 ggcagagatc gcggcagagg ctgttcgcct cggagttcct gtcgaactca cctggtcgtg
    16261 ctacgatcca caggcaagcg gcacgagcct ggtgccgtgc cgcaactgcg atgcatgcat
    16321 tctgcgccgc gatggcttca tggccgcagg cctcgctctc agccctgaag aaatttacgg
    16381 ttctccgaca gaacgcgcat aaagcggctt gcgggaaatc ccatcacgtt gaagtaacaa
    16441 ccctcgaagc ggcggataca cgccatgcca aaggcatcct gcgcgccata accgccggcc
    16501 ttgtcgtaag gttggggcga ggcaatatac gactctatag tttcagtact gagcgcatca
    16561 aaaaaaacgc gggtctcttc gataaaccgc aggcgctccc cctttgggaa aaccaccgcg
    16621 accgcggtat agacgatatg atcgcgcccc gacaggctct gcagcatcga acgcgcgtgc
    16681 cctttgtcgc ggggtttttc aataacttcg ccatcgagca ccaccacagt atcggcggca
    16741 acggtcagtc tgccaggcag ggcgacgaca tcggcttttt tttctgccag caaaagggtc
    16801 tgctctgcag catcggcatg ccgaatcgac tcttcatcaa tgcccgccgg gcgaatctcg
    16861 catgtgaagc cgagccgctc caaaatttcg cggcgccgcg gcgacgaact cgccagcagc
    16921 aggggagttt tagtcagtgc gctgaacatg gtaccttgct atcgaattga cgctgcgatg
    16981 aggcagaaag atcaaaccgg catgggttac ctcgtagcgg caggcatgtc gggctttttt
    17041 gcagtcttct ttggcgcatt cggttcgcat gtcctgagag cgagtttaag tgaaaaagcc
    17101 ttttcggtat tccagacggc aaacgcctac catttctggc atacactggc gctgctggca
    17161 gttgcactgc tgcgcatcgc acgcccgcag aaatttttca cctcgtctgc catagccctg
    17221 ttggccggac tctttctttt cagcggcagc ctctatctct atgccatcta ccaaattccg
    17281 gtttttgcgc gcctggcgcc actggggggc agtgcctata tgctcggctg gctgttcctg
    17341 gttttgggtt cagtgcgcgc cttcgcgcgg agctcggaat aacacagggc tataatcgct
    17401 gattttcggc tcctattcag actgaatagc ctgcgttgat ccggaagaat accctgcatt
    17461 tacttcccat aataaatatt atgtctcatc attagcagga caggttgaga gtatcgacac
    17521 agagtttgca ccctaagctc gatacacacg tccctgtcta gcacagccgg tgctcgccgg
    17581 atttctgctc caggctacaa tctcgcctta ggccgatctt ccccggctga agaccaacca
    17641 ggcagcgagg cacaacaggc ctcccaaaac aaacggtagc ggcaaccaga cggcgtacag
    17701 gcccgttgca gcgaccggcg cgacaaagcg tgccatggcg gccaggccct gcatgttacc
    17761 catcgtctga ccctgctcat ttttgggcgc attcagcgag gcaaatgcgt tcagtgatgg
    17821 tgttccaaag cccaaggcaa gagacatcag caccagtaca accgacagca aaacggtctt
    17881 gccggccatg ccaaatactt ggcccagctc gagacctcca atccatggca ggacggccat
    17941 gccgacagct gaaccgagca accccagctg cacccaatca gccggtggcc ggcgcttttc
    18001 gacccggcga tagattccac cttgtaccag tgccagcaag atgccctgga aaccaaagaa
    18061 atagtatgtt tctttaagat tgaagccgta ctggcgctgc agaatccacg cgaaaagcac
    18121 ctcgaaactg acaaaactca gctgcgacag aagttgcgtc gcgtaggtgg agctctgtgg
    18181 tggtcggaaa actgaccgca gaccagccag cacgccgccc ttctctgtgg attcgtgcgc
    18241 cataccgaag tgattaaata taatcaatat aaccgatacg gaggctagca gaagcggaac
    18301 aacaccaatc tgccagaatg gattaatatt aatcgcaaat tcatgattaa tattaaccat
    18361 taatccgccg ataaatggcc ctaaaacaaa acccatccca atacacgcac ccaacaaacc
    18421 catcgcccga ggccgttccg ctggcgcagt gttatccgcg agaatgctct gcgcaatggc
    18481 aatattgcct ccggttattc cactcaagag cctgcccaaa agaaacggcc aataagagct
    18541 cgagagtatc cagactacat agctcagaag attgcctaca gccgagacca ggaacactgg
    18601 tttgcgccca tagatgtcgg ataaccgccc taaaatcggg gcactgatca tctgagagcc
    18661 tgaatacgca gccatcagca gccccatgaa tatctctggc gacggccagc tcagccccga
    18721 attcttcacg agggctgttg ttagatccct aatagccggg atcataacgc caaaacccag
    18781 catatccagg aataccacta aataaacggt aaaaaagcct gctttgctca ctcattcatg
    18841 tgccaaaacg gactcaggcg gtcaaaaaca ccgcgcgaaa tggccttgac tgtatatatt
    18901 ctatgtagaa tattctatat agaatatgaa aacgccttca gatttgcgcc tattggccct
    18961 ggcgatcctc caacagagcc caaccgaacc gaaaacccga taccaagtta tgattgaaat
    19021 caatcaaatc atctctgaaa tcttgccgcc ctactcgcca ggggctgtat atcacgagct
    19081 caagctgctt aaaagaagca acatgattga tattgatcac acctcccgtg tcgtcattac
    19141 tgaaccaggg ttaaacttac tgcaccaaag cctcttggaa gccccaaccc caaggccact
    19201 cctctcgctc ttggttagag tgctgactct tctactactc aacaataaga ctcagaagga
    19261 aaaggggcta cataaactgc aggtggagat gattaaattt aatcaagaag tggcagtaaa
    19321 tgagcgctgg tcgagtgacg acaaactgtc gctaataacc aagctattgc aacttggctt
    19381 atccatgcga aagactatct cggcatttgt cgctcaatta tgagctttct aaatgtggct
    19441 aaagcctggg gtttctcgga caatattagg gcgcaaagct tatagcgcct aattcttggt
    19501 catgtatggt tgccctgttg agaggtacca gtagctcgag gccagcggga tgaattttgg
    19561 aacgaacata gacattttga ctaaatatca tcacctcaag gggctgcaca agggcacgct
    19621 ggggtaaccc atttcgatca aaaaagtcga cctttagtat ggcttcctgg cgtatttttc
    19681 cgagaaaata atgctcaatc tggtcaaaat ttttcggaaa aagtatttcg tatttttctg
    19741 tcttacgctg ccacctgcgt atccgctgga actttgtttt cgaagaaaaa atatctggct
    19801 cagttaacag aaggtgcgtg aaggccagcg ctgttgcgcg cgcatcggcg agcgcacggt
    19861 gcgcctgccc tgtttcgatt cccaactctt tcttgatcga ttcgagtgaa tgcgacatgc
    19921 gctcagcgcg caacttgcgc gacaactgca ggtggtcata ataactgcga ttgggccacg
    19981 gtcgcttcag tgaaccgtaa gcctgcttca ggaatgcaag atcaaaggga gcgttgtgga
    20041 agacccagct gccatcggtc atgaactgca tgaacgcaga cactccctct tcgagagaaa
    20101 ctgcgttggt cgcgagttct tctgcgctga tctggtgtat cgcgcgcgcc ttcggatcca
    20161 tcttccgttc gggcttgatg aggatagaga aatccttatc cagtccatcg agcgtgaacg
    20221 agaccgcgcc tatttcgatt atttcgtgtt tatacggatt gagcccggtt gtctcgagat
    20281 cgatcgccac gaagcgcatt tcgtgcgccc attgctccat gcgccagcag ctctgcggcc
    20341 tttaccgggt cgattgtaaa atgctttata gacagctcgt attctttggt cacggcagac
    20401 cctacggcat ggcgagccgg gtaaggcaag ggggagctat cgcgtgctgc ttctctgccc
    20461 tgtccaaggc tgcacgcctg gcgatatacg ttgcctttgc cgtcttcgcg ctgctgtgcc
    20521 acgcagcctg cagggttcgc aaacccacgc gcagcagcct cggcgacact ggacgcatca
    20581 aggcggctta cctgcataga gaaggtcata gcacctttat agtgcggcgc acaaaaaatt
    20641 tccccattgc tctcgctgag tatctctggg tgtaatacac ccctcgggta cgtgcagact
    20701 ggcagttgag atatcttctt tagaatgaat ttgatttcag ccagcttctc aagcatgggg
    20761 gcatgcaggg cagttctgac cggctgcata cctgcaggtg accggtatgc gctccctttg
    20821 gcgagctttt tcttcgcctg aaacggaaaa ctgcgggcaa cctggttttg ccgggggtgc
    20881 ttttgggtca gggcgtatgc aacctgcttt acgagggatt tccccaaccc caaggccgaa
    20941 gctttcagta ccgaggtaaa tccaccggat tttttttgct ctaaactctt gctttttggg
    21001 acgatatgca gatcagggga aacccggtct gtcgccggcg cacttgcaca
//
LOCUS       DAOQJF010000008        20072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1226_length_20072_cov_5.809912, whole genome shotgun sequence.
ACCESSION   DAOQJF010000008 DAOQJF010000000
VERSION     DAOQJF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 20072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20072
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1226_length_20072_cov_5.809912"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="PLF85_00935"
     CDS             <1..1876
                     /locus_tag="PLF85_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE18246.1"
                     /translation="VSTLDQLLNLTGELVIANSGLLSLSERSARIGTDAEFAIELSDK
                     SREIFRVAAEIQNIVMKSRMLPLENVFSRFRRFVRDYSDKSGKAIRLEISGAETELDK
                     RVIDEMVKPLTHLIRNALDHGIEESAERIAAGKPPEGTLRLAASQSGSSILITVEDDG
                     RGLNVARIIERAVSRNLVSPERAAALSLPEVYEFIFAPGFSTKESADELSGRGFGMDI
                     VRDSIHKLSGDLQLFSEPGKGTRMVVKLPLTLAILTALTVRVRNDLFAFPLSTIEESI
                     EIPSGSVMRVDGRDILHWRDQILPFLRLDNVLGYPPIEETREHLYAIIAEIHRTRIAI
                     GVDEFLQKRELVIKSLAEHYQHVPGLSGASMLGDNEIVFILDVEEIARMHANRYAHFT
                     EPAPEQPREKNSRQSDGISESAMPEGPDGTVLQADPEAAEPTMKPLFDFSDKELMRKW
                     IAQSNKTAVQGIQMLTGNQAISVKKSRGSRVKAERSADTMGKITERANDILLIHLPML
                     PAAGAIDLILDKKSAERMASLLFKAAGFESMEEFDPSPLLEITNILGSAYTNTLTFLT
                     EKSVEPATPVLLSTREAIEELIDARRQSPKSEMLVVENQFHIDDEDIQVELVIYLTGE
                     "
     gene            complement(1906..5265)
                     /gene="carB"
                     /locus_tag="PLF85_00940"
     CDS             complement(1906..5265)
                     /gene="carB"
                     /locus_tag="PLF85_00940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNE18247.1"
                     /translation="MPRRSDIKKIMIIGSGPIVIGQACEFDYSGTQAVKALKKEGYEV
                     ILINSNPATIMTDPELADRVYVEPISLPYLTRIIEKERPDALLPTVGGQTALNAALTL
                     SAAGVLKQYGVELIGANEAAIKKAEDRKLFKEAMEKIGAAMPRSGIAHTLEEALALLE
                     NIGLPAILRPSFTLGGSGGGVCYNREEFISMVSKALTESPTSQVLIDQSILGWKEYEL
                     EVMRDKADNVVIICSIENLDPMGVHTGDSITVAPQQTLSDVEYQNLRDLSIKIIREIG
                     VETGGSNIQFAVNPRDGQIMVVEMNPRVSRSSALASKATGFPIAKIAALLSVGYTLDE
                     IANDITKKTPASFEPTIDYVVTKIPRFTFEKFPETPRVLDTQMRSVGEAMAIGRTLPE
                     SFQKAMRSLETGRYGWGADGNLEIELEILRMGDETTQRRHILEKISVPSDTRILYVRE
                     ALLRFSLEEVYQRTFIDPWFLTQLKRILDMEQRVTEAFRKNKTLDAALLREAKQMGFH
                     DRQLAYLANFDTAQGMAKELSRLEQGSSAFMSKMHELVQLLSKSEREISEQRNRSGEK
                     PVYKRVDTCAAEFESFTPYMYSTYETENEAQVSDREKVFILGGGPNRIGQGIEFDYCC
                     CHASYALQEVGLESIMVNSNPETVSTDYDTSDRLYFEPLTIEDVMHIYEQEKQQGKLR
                     GMILQFGGQTPLKLAKALSERGVPILGTSVESIERAEDRKLFNELIEKLKLRQPRGRM
                     AASLEEALAAAQEIGYPVLARPSFVLGGRAMMIVHTEDQLRQYMQTSVEVSRERPVLI
                     DQFLSGAQEVDVDAICDGTDVFIAGILEHVEEAGVHSGDSACILPTKNISETILAELR
                     RVTRAIALELKVIGCINIQFAIVDDVVYVLEVNPRASRTVPFISKAIRVPLAKIATKV
                     MLGSTLKSLGLTKEILPEHYYVKEVVLPFKKFSGADTILGPEMKSTGEVMGIGRTVPE
                     AFYKAQIAAGQKLPAKGTIFVSVNDSSKAPLMDALKAAQKAGYRILATEGTALSLNAA
                     GITTERVNKVHEGRPHVVDSTKSGDVQLIINIPTDIKTRNDALEIRLSALQYSVPYFT
                     TVEAAREAIFGMVEMNGKPEEIYVLQESKNR"
     gene            5389..6138
                     /locus_tag="PLF85_00945"
     CDS             5389..6138
                     /locus_tag="PLF85_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18248.1"
                     /translation="MALMLVAVAFAIPTPALAGLFGETRHHLFFVEGSLGGGWNAAFD
                     RERSLVDAYGDFYWYSGGNYYKYQPSNLVLTAGFGYEYLLLPFIGLRASYSYTSYLND
                     WTATRGTGRNRRSSSAGGQVAETSGYFVGPVLRYPLPWIAGLYIEAPILWGIHRGSYY
                     PLAAYSEFRSETGRMPQHPDEYTTQPLVVTKWRYGLGLAFMREGFPLFASLQFLMESG
                     RTTTSNGPEQVLPAGSAWDAFIATVAVGWRL"
     gene            6187..7401
                     /locus_tag="PLF85_00950"
     CDS             6187..7401
                     /locus_tag="PLF85_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18249.1"
                     /translation="MRWPKPAKLEQWFTMNDLDLMTEIEKLAHVLRVSPDRIQYLKHL
                     SLDDFRSLNDSISNRLLTDSSDVWSKLAGVAKFMPNFINAQVAKSLLGPNITANLTYH
                     IDTKQAVSIASSLNIEFLAQVAENLVPARAKHLIEAFPMDVLVKLTRTLTRDKKFFTM
                     GAFVDYMDREKILALSREIPDTESLLRVGLYTQNKEHVAKLADGFSDEKVIDMIETAQ
                     QKDLWPSLIGLLAHFTETQHRRLAGLVGRVSVDALLGMVPFAIEHGALVHIFAFLQYL
                     NPDMHAHMATIVAKLDSEALSRIIHAADESGKLPVALKIADHLAERDVDRLAAMSHRF
                     DDELLKRVIVTAHAEGLVPFGLRLLALLPEGEIPRAEKIAREIDRKVITDAQAQIEQS
                     LLKERLGWLKKI"
     gene            complement(7417..7686)
                     /locus_tag="PLF85_00955"
     CDS             complement(7417..7686)
                     /locus_tag="PLF85_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18250.1"
                     /translation="MNPFRLSTRLVTPLASAAFEAWLKQSLKTCAKLGGSVRQPWEKQ
                     NPVSRACLLGQAAISAKANAEALRAEWALIEKSANPYVQAPDKSD"
     gene            complement(7860..8834)
                     /locus_tag="PLF85_00960"
     CDS             complement(7860..8834)
                     /locus_tag="PLF85_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNE18251.1"
                     /translation="MHTNEPHAWSSNIGVRGMLMNLAQPKPACIADYVLLYAPLGILP
                     HLPNAAAACVGKFSLTGAISVNSVLSTCGAGVNCQKFTLTTTTQSAATPYTLTAVPNV
                     ATDLAGRTIDTVNTATYTSGDVMSNLLAHYKFDGNANDTQGTYNPTFNSATLANDRFG
                     NSNRSYNFSGTTRIEVPPINFNSTGVSVSAWINFTSIGAFSSIARDDSATIALTLTPT
                     VAAFQLNLPGFSYHNVNAAINAADYTGGNWHHIVGTYDGAFFRIYKDGAEIGNSAYNF
                     AGTLSTGTVQAIGGTASGENCFGRIDEVRFYNRGLTAAEVASLYLLEK"
     gene            8837..9766
                     /locus_tag="PLF85_00965"
     CDS             8837..9766
                     /locus_tag="PLF85_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18252.1"
                     /translation="MRHVFLSFFLLGTIPPVVAQEKPAAPGKKIFSINITPPPDTSAT
                     ELEFSEIDLTKATPEQATQARTKVIRTTEKAYREYVAPELRFFRVRSIHKTGVPGAFG
                     KTLLVQDYLRKPYREPQLPLVQQGKTEYLLGSRIELPQQPGLVTRYRLDEGESLEYNE
                     PLVFNKPGIYRMQVSIENEAKEAVLARTYIFKVELNAPTTRAVISDPVHGKRGIKLGK
                     DSALLFLATDAESGVAKTFYRLAPIGKNIEEMPFKEYGKRLSFGEVSAGGAINLLQYY
                     SVDVAGNKEELRSEVVFCETASESAPATKDTSQ"
     gene            complement(9744..9920)
                     /locus_tag="PLF85_00970"
     CDS             complement(9744..9920)
                     /locus_tag="PLF85_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18253.1"
                     /translation="MPLVVNPGRWRMVVLSFPFWLIFAILSPVLVLFFALGLGLWFQP
                     QMQQKPAIIEKYPS"
     gene            complement(9942..11741)
                     /locus_tag="PLF85_00975"
     CDS             complement(9942..11741)
                     /locus_tag="PLF85_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18254.1"
                     /translation="MQKRLERYLPYSIDEIEASAGSINFNPRLVAEDLGQKLNQFYLS
                     DPSEKKIANRQDNDSNAAWYRKEWNVNLQRLFMRDLLAKTQPSFIIKEAPNIALLHYN
                     YALAQMKLKKPYHAAFHFVQSLRYRSLRMDDEIYTNTDRLQLVKAGAAEITDADSYRE
                     TKKQLFEKEREKQRLEEELVLLDDRSRSPALGRATTATNVEAERAKQQQLIADQKEKS
                     RQALERVRGEYDKAKAGFQQAQQKYTEHAREYNRKSADMLIEMAGLVRQLEDAIKERS
                     KVLNKKALYKTNFNQTLQHDYSQNRQFTAYANILEAATRLDPENPVTNFTLAEEYRTS
                     QDVSRSIDSYERALAANDKPRTGFELKEEQVRKSNAALGALYYSKSRFVDAAFFYEKA
                     YSTTTENKEKQNLGWQLAKLHVEKTGNYERATELFEAYLKEIAALNPTDVADRTQWIK
                     ERFTANRYLLHMHSKKNAQEKVLSSMQAAAAAHADLETLITDLRARNAKTYQEMQTAK
                     KSLFDDVRSRDLNVYLKLEAEFEKQKRELAIIEAGRNSLPLAQFYSQYARFLEGRHDI
                     AGAMDVYATAEKLGLGADHARREQGRLKAQYLQ"
     gene            complement(11964..12197)
                     /locus_tag="PLF85_00980"
     CDS             complement(11964..12197)
                     /locus_tag="PLF85_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNE18255.1"
                     /translation="MDLSCLMRPRKLCLCKGVSRADIQAAVRAGADTFPKLIEKTHAT
                     TGCGTCYHEVYAAFREEREQERCRLTGQMGLKF"
     gene            12496..12684
                     /locus_tag="PLF85_00985"
     CDS             12496..12684
                     /locus_tag="PLF85_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18256.1"
                     /translation="MDKSWCTKPARDHVLIVGEYYHVTRESYPYKTLQAYVEPAVRIH
                     RQTGELLLPEGLRATSSD"
     gene            complement(12668..13417)
                     /locus_tag="PLF85_00990"
     CDS             complement(12668..13417)
                     /locus_tag="PLF85_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18257.1"
                     /translation="MRSLRLTILLFGGIFLSACSSLPKGTPGPRAEELAQKMLAGADV
                     DKWQATAAVSFTFRGGDKIFWDRKRKLTEVIIKKKLVQFSEVSGKSVCFEEERRVTDD
                     CADMTETAIKRFYNHTFWLNPAFHISSPGTERALVGDNQLLVSFKSGGSTPGDSYLFT
                     LDEEGKIAEMRMWVSVLPIKGARAVFSNYTKTQTGVRVALNHDVASLASVNLSDVVMY
                     AQYPEKGGVDRFQGLLEIGGLPASESINRKK"
     gene            13525..13992
                     /locus_tag="PLF85_00995"
     CDS             13525..13992
                     /locus_tag="PLF85_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18258.1"
                     /translation="MRIQDWLKLSALVATAGFVKWSAVVESAWLYRVFLYVPAALLSV
                     FGYGDYGESGGVYTFETFVLDYSCAGVNFFLITLAVGCLAMLSAGRFYLIMIPIAWFM
                     TLAANTLRVGLYLKLELLAGHRPWLHEATGILVFLTLLVVYYHLLMRRLHEEQ"
     gene            13979..16609
                     /locus_tag="PLF85_01000"
     CDS             13979..16609
                     /locus_tag="PLF85_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE18259.1"
                     /translation="MKNNNRRDSGLLALAALVPAAISLAIAVPMLWIIWPDLSPRQID
                     SVISLLLLTHGLAFGMGLISAWLWLRRKDLPISLLIVTAPAQFAAFYIQLRWLNVPPP
                     RGWMFDEDAFMTLVLSGIIPLFYTSLLLLATRFSIHNRRGLFISAAGSIGIPLCIYAL
                     FQLMRYARGGSGADAMLIVVLASLTIAFSFLVLRLLLFLIQRRAGWLTTTRGRFSLRL
                     TFIGLLPLLGLLMNTIGPVARESRTVLGDFTGLPFWLLAVVNALVYLLPLGRNLFANA
                     LLVVLRGVGLCFVVYFCVVFLLYLPLALLLIAALGLGLLLLIPYFTLGIQWIILREDY
                     TQWRENSRAWLFPVLLAGGFLLLPCTLLADMLLQRSQLIAAVAYVQHPPLTLNHDADF
                     NRSRIESLLAEPRRQSRGWLDQRESIPLYDTLRQQVVYDGVGLAAGMRQRLRHIFLGE
                     ENAETSASNTLPEGRVGAVTLSARQDGNVTYTDLRIQLENPDARREAELDVRVALPDN
                     AFLAGHWLVIGQVEQPAQITSRSAAIWTYNRITEQRRDPSLIWYESKNRLRWRLFPIP
                     ANGRREARLQIAHLGQADLRLGSQSVRLHGKEKTAETNAVLPAQAPRPYLQFIVDCGN
                     KGMRDYSSLVQAVAQATRIPLSGARISFVNASIRTIDLQSVSDARCEPAREGFFADLA
                     FRALMAEKNRAASSGFPVFVVLSATLPGANWSDISYFTRDYRDMDFFIHADGKGLHAY
                     DFYGNRTRLPGFSNGVTSQRRQRAQPAGMEKFLHGQELYERWLRGENPARHEAIRTAM
                     HTGILNPAAASIVLETEAQRRKLAEMNQRTLKSQHELETGNRMRMSEPTTALLLLLLL
                     PFLVRRLGGGNSLKKNDLSG"
     gene            16688..17092
                     /locus_tag="PLF85_01005"
     CDS             16688..17092
                     /locus_tag="PLF85_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18260.1"
                     /translation="MSLVNITFALSAPAKRAWENLSNHRHFDQVAELFFDAMRHERYR
                     NFVVKPHFFTEVHGHRRARKHSLRSPGEKLHRVRLVMQLQTLQKMKRLENLGLALSWQ
                     VEEILYFGFCRGLLPRMNPYYSLETNDTCVTK"
     gene            17128..18165
                     /locus_tag="PLF85_01010"
     CDS             17128..18165
                     /locus_tag="PLF85_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18261.1"
                     /translation="MQVQLEKARASANFTRFRGEKSGGHYESYFMRANNAETGQAFWI
                     RYTIYQNETGKGTKSPMGELWAIWFEKGKAPVAAKSEFPLSKAEYSNLQFSLNFDGAT
                     LDANGLKGAANNCGTNEISWDLQIAPMSKVRNLTPLFPMPLASYDTPLPRAKLLVAQP
                     FVTFKGQLAVNGKKIAIQDWQGSQNHNWGSRHTDEYAWGQVAGFDNEPDAFLEVASAK
                     LKFGPLMTPFLTPMVLRFHGKDYCLNTYWQAIRNQADLEYFRWSFSGESKDLKIEGKI
                     SANREDFVALNYYNPPGDVKTCINSKIASCEVTLTEKSRIGVSTRLVTKNRAAFEILT
                     SKTDHGFTPLF"
     gene            18215..18598
                     /locus_tag="PLF85_01015"
     CDS             18215..18598
                     /locus_tag="PLF85_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18262.1"
                     /translation="MVIKLIVMFALFCALLYLLNKAMPDFHIEMGAIPVVALILVGVN
                     VVVGALLGGTATVLNVLTLGIIKWILQFITLGLFGLLVSFVFNVIIFWVADKLTDRLT
                     IKTMRTLLVSAGALQFTNFLLNRVF"
     gene            18622..18873
                     /locus_tag="PLF85_01020"
     CDS             18622..18873
                     /locus_tag="PLF85_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18263.1"
                     /translation="MLLASAVGAQTAQKGKKTECRLSRYQPQAPALADLNLEEAEKRE
                     IVQLIQEGRKIDAVLIMRKCRDITLKEQKMIVDAVQKNF"
     gene            18810..19325
                     /locus_tag="PLF85_01025"
     CDS             18810..19325
                     /locus_tag="PLF85_01025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE18264.1"
                     /translation="MPRHHAERTEDDRRRSTEELLIPAEIQVTQVDRGAAQQVFELDR
                     EIFGEGCWSHQQWVSEFASPGVIILLAVQAGQPCGFLSSGIAGDDLEIRKVGVLPRYR
                     RHGLGRRLLQQATMNTANTARRCLIEVSSANAEGLAFYRRTGFTEIGQRTSYYADGST
                     AIVMEKILSVP"
     gene            19347..20036
                     /locus_tag="PLF85_01030"
     CDS             19347..20036
                     /locus_tag="PLF85_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNE18265.1"
                     /translation="MIRNPFDTLGSIVADDEEGTKKKEERPPALRERIAERFLDKRQI
                     FLWGAVTDKSAEMIVERMLFLEATDPGKPIYFFINSPGGVITSGMAIYDVMRMISSPV
                     YTITMGMAASMGAILLTVGEKGHRYVFEHAKVMIHQPLISGQIVAPAIDIKIHAEEIK
                     KTREELNRIIADTTGQPLTRVEKDTDRDYYLDGKGAVEYGLADKVLTDFSELGLFDTK
                     PAKAAKPKKTK"
BASE COUNT         4401 a         5603 c         5486 g         4582 t
ORIGIN      
        1 ggtcagcact ctcgaccagc tgctgaacct gacgggcgaa ctggtgattg ccaatagcgg
       61 gcttttatct ctgtctgagc ggtctgcgcg tataggcacc gatgcagaat tcgcgattga
      121 attgtcggat aagagccgcg aaatattccg cgtcgccgca gagatccaga atattgtgat
      181 gaaatcgcgg atgttgcctc tggaaaatgt gttctcgcgc tttcgccgct tcgtgcgcga
      241 ctattcagat aaatcgggca aggcaatccg cctggaaata tccggtgctg aaacagaact
      301 cgataagcgc gtcatcgacg aaatggtcaa acccctgacg cacctgatac gcaatgccct
      361 cgaccacggt atcgaagaaa gcgcggaacg tattgcagcg ggtaaaccac ccgagggcac
      421 gctgcgcctt gctgcttcgc agtcgggcag cagtattctg atcaccgttg aagacgatgg
      481 cagaggacta aatgttgcgc gcattattga acgcgcggtg agccgcaacc tcgtaagccc
      541 cgagcgcgcc gcggcgcttt cgctgcccga ggtctacgag ttcatatttg cgccgggctt
      601 ttcaacgaaa gaatcagccg atgaactttc cggccgtggt ttcggtatgg atatcgttcg
      661 cgattcgata cataaacttt cgggcgatct tcagcttttt tccgaaccag gcaaaggcac
      721 gcgcatggtc gtcaagttgc ctctcacgct ggcaattctc actgcgctga cggtgcgggt
      781 gcgcaatgac ctttttgcct ttccgctgag caccattgaa gaaagcattg aaattccgtc
      841 gggcagcgtc atgcgcgtcg atggccggga tatactgcac tggcgcgacc agattctgcc
      901 attcctgcgg ctcgacaatg tactgggtta tccgcccatt gaagaaacgc gtgaacacct
      961 ctacgccatc atcgccgaaa ttcaccgcac gcgcatagcg attggcgtcg atgaattcct
     1021 gcagaagcgc gagctggtca tcaagtccct ggcggaacat taccagcatg tacccggtct
     1081 ttccggggca tcaatgcttg gcgataacga aatcgtcttc attctcgatg ttgaagagat
     1141 agcccgtatg catgctaatc ggtatgctca ctttacagaa ccggcaccag agcagccgcg
     1201 ggaaaaaaac agtcgtcaga gcgacggcat tagcgaatca gcgatgcctg aagggccaga
     1261 tggtacagta ttgcaggctg acccagaagc tgcggaacca actatgaaac ccctgtttga
     1321 tttcagcgat aaagagctaa tgcgcaaatg gatcgcgcag agtaacaaga ccgcagtgca
     1381 gggcatccag atgctaacgg gaaaccaggc tatttccgtt aagaaaagcc gcggctctcg
     1441 ggttaaagcc gaacgttcgg ctgatacaat gggaaaaatc accgaacggg caaatgatat
     1501 cctgctcatt cacctgccga tgcttcctgc cgcaggcgcg atcgacctaa tactggataa
     1561 aaaatctgcc gagcgcatgg caagcctgct cttcaaagcc gccggttttg agagcatgga
     1621 agaatttgat ccgagcccgt tgctcgagat cacgaacatt cttggcagcg cctataccaa
     1681 cacgctgaca ttcctgaccg agaagagcgt cgaacctgcc acacctgtgc tgttgtcgac
     1741 tcgcgaggcc atcgaagagc tgatagacgc gcggcggcaa tcaccgaagt cagaaatgct
     1801 cgttgtagag aaccagtttc acatcgacga tgaagatatc caggtagagc tggtgatcta
     1861 cctgacaggc gaatagattt agcaggctcc tttttcagca gcctgctagc ggttcttgct
     1921 ttcctgcagt acgtagatct cttcgggttt gccgttcatc tcgaccatac cgaaaatcgc
     1981 ctcgcgtgct gcttcaacag tggtaaaata aggcactgaa tactgcaggg ccgagaggcg
     2041 tatctcgagc gcatcgttgc gtgtcttgat gtcggtcgga atgttgatga taagctggac
     2101 atcccccgac ttggtgctgt cgacgacatg cggcctgccc tcgtgtacct tattcacgcg
     2161 ctcggttgtg ataccagctg cattgagact gagcgcagtg ccttcagttg ccagtatgcg
     2221 gtaaccagcc ttctgcgcag ccttcagggc atccatgagg ggagctttcg acgaatcgtt
     2281 tactgatacg aagatggtac ctttcgccgg cagcttttgg cctgcggcaa tctgcgcctt
     2341 gtagaaggcc tcggggaccg tgcgaccgat acccatcact tcgcccgtcg atttcatctc
     2401 tgggccaaga atggtatcag cacccgagaa cttcttgaac ggtaatacaa cttctttgac
     2461 gtaatagtgc tcgggcagaa tctctttagt gagccccagc gacttcagcg ttgacccgag
     2521 cattaccttg gtcgcaatct tggcaagcgg tacgcgaatc gccttcgaaa taaaaggcac
     2581 cgtgcgcgac gcacgcgggt ttacttcgag cacataaaca acgtcatcga caatcgcaaa
     2641 ctgtatgttg atgcagccga tcaccttcag ttcaagcgct atcgcccgcg ttacacgacg
     2701 cagttcagcg agtattgttt ccgagatatt cttggtgggc agaatgcagg cgctgtcgcc
     2761 cgagtgcacg cctgcctctt caacgtgctc gagaataccg gcgataaaga catcggtacc
     2821 gtcacagatg gcatcgacat cgacttcctg cgcgccggac aagaactggt cgatcagcac
     2881 cgggcgctcg cggctcacct cgacgctcgt ctgcatatac tgccgcagct ggtcttcggt
     2941 gtgcacgatc atcatggcac gcccgcccag cacgaacgac ggcctggcca gaaccgggta
     3001 accaatttcc tgcgctgcag cgagcgcctc ttcgagcgag gcggccatgc gtccgcgcgg
     3061 ctggcggagc ttcagctttt caatgagctc gttgaagagc ttgcggtctt cagcgcgctc
     3121 aatgctctcg acgctggtac cgagaatcgg cacaccgcgc tcgctgagcg ccttcgcgag
     3181 tttgagcggg gtctgcccgc cgaactgcag tatcatgccg cgcagcttgc cctgctgctt
     3241 ttcctgctcg tagatgtgca tcacgtcttc gatcgtgagc ggttcaaagt acaggcggtc
     3301 tgaggtgtcg tagtcggtcg aaaccgtttc agggttcgag ttcaccatga tactttcgag
     3361 gccgacctct tgcaacgcat aactcgcatg gcaacagcaa tagtcaaact cgatgccctg
     3421 cccgatacgg ttgggacctc caccgagaat aaaaactttc tcgcggtcag aaacttgggc
     3481 ctcgttttcg gtttcgtagg tcgagtacat gtaaggcgta aaagactcga actcggcggc
     3541 gcaggtatcg acgcgtttgt agacgggttt ttcacccgaa cggttgcgct gctcagaaat
     3601 ttcgcgctcc gacttagaga gcaactgcac cagctcgtgc atcttgctca tgaaagccga
     3661 agaaccctgc tcgaggcggg aaagttcttt cgccataccc tgcgcggtgt caaagtttgc
     3721 aaggtaggcg agctgccggt catgaaagcc catctgtttc gcttcgcgca gcagagcggc
     3781 atcgagtgtt ttgttcttac gaaacgcctc ggtcacacgc tgctccatgt ccagtatgcg
     3841 cttgagctgg gtcaggaacc agggatcgat aaaagtgcgc tggtaaacct cttcgagaga
     3901 aaaacgcagc agtgcttcgc gcacgtagag tatgcgcgtg tcagagggca cgctgatttt
     3961 ttcgagaatg tggcggcgct gcgtcgtctc atcgcccatg cgcagtattt cgagctcgat
     4021 ctcaaggttg ccgtcggcgc cccagccgta acggccggtc tcaagcgagc gcatcgcctt
     4081 ctggaaagat tcgggcaggg tacggccgat cgccatcgct tcgccgactg aacgcatctg
     4141 cgtgtcgagc acgcgcggcg tctcgggaaa tttttcaaaa gtaaagcgtg gaatttttgt
     4201 cacgacgtag tcgatcgtcg gctcaaaact tgcgggagtt tttttggtga tgtcgttggc
     4261 aatctcgtcg agggtataac cgacagacag taacgcggca atcttcgcga tcgggaaacc
     4321 cgtcgccttc gaagccagcg ccgaagagcg agacacgcgc ggattcattt cgacgaccat
     4381 gatctggccg tcgcgcgggt tcaccgcgaa ctggatgttc gagccgcctg tttcgacccc
     4441 gatctcgcgg ataatcttga tcgagaggtc gcgcaggttt tggtactcaa cgtctgaaag
     4501 tgtctgctgc ggcgcaacgg tgatcgagtc gccggtgtgc acgcccatcg ggtcgaggtt
     4561 ttcaatactg cagatgatga cgacgttgtc agctttgtcg cgcatgacct caagctcgta
     4621 ctctttccaa cccaggatac tctggtcgat cagtacctgg ctggtcgggc tttcggtcag
     4681 cgccttagaa accatcgaga taaattcttc gcggttatag cagacaccac cgccgctgcc
     4741 gccgagggtg aatgacgggc gcagaattgc cggcagaccg atgttttcaa gcagcgcgag
     4801 cgcttcttcg agcgtgtgtg cgatacctga acgcggcatg gccgcgccga tcttctccat
     4861 cgcttcttta aaaagtttgc ggtcttcggc ttttttaatc gctgcttcgt ttgcgccaat
     4921 cagctcgacg ccgtactgct tgaggacccc tgcagcagaa agggtcagcg cagcattgag
     4981 tgcggtctgg ccgcctacgg tcggcagcag tgcatcgggg cgctcttttt cgataatgcg
     5041 cgtgaggtac ggcagcgaaa tcggttcaac atagacccgg tcggcgagtt caggatcggt
     5101 catgatcgtc gccggattcg agttgatgag tataacttcg taaccctctt ttttgagggc
     5161 cttgacggcc tgcgtgcctg aataatcgaa ttcgcaggcc tggccgatca cgatcggccc
     5221 cgacccgata atcatgattt ttttgatatc ggaacggcgc ggcacgagtc atttcgcgcc
     5281 cgcgagatat gcggtcaaag atagtggtat tctgctgtcc tttcgtggac ctcaagcgtc
     5341 taactctgta tagtgggcag aaacagtacg ataaagctcc cccggcttat ggccttgatg
     5401 cttgtggcgg tagcttttgc gatacctacc ccagccctgg ccggactttt tggtgagacg
     5461 cgccaccacc tgtttttcgt cgaaggttcg ctgggtggcg gctggaatgc tgcgtttgac
     5521 cgggagcgtt cgttggtcga tgcctatggc gacttctatt ggtactcggg tggtaattac
     5581 tacaaatacc agccctcgaa cctggttctc accgcgggct tcggctatga atacctgctg
     5641 ctgccgttca ttggcctgcg cgcctcgtat agctacacct cttacctgaa tgactggacg
     5701 gccacgcgtg gcaccggcag gaaccggcgg agctcgagcg cgggcgggca ggttgccgag
     5761 acgagcggct attttgtcgg ccccgtgctg cgctacccgc taccgtggat cgcgggtctc
     5821 tacatcgagg cgcccattct ctggggcata caccgaggca gttactaccc actggcagct
     5881 tacagcgaat ttcgcagtga gacgggtcgt atgccacaac accccgacga gtacaccacc
     5941 cagcctttgg tagttactaa atggcgctac ggcctgggcc tcgccttcat gcgcgaggga
     6001 tttcccctgt tcgcaagcct gcagttctta atggaatcag gtcgcaccac cacaagtaat
     6061 ggccccgagc aggtgctacc ggccggttca gcctgggatg cattcatcgc gaccgttgcc
     6121 gtcggctggc gactgtagag gtatgttccg cttgaaggaa cctgctagct aaataatgac
     6181 gctcttttgc gctggccaaa acctgcaaaa cttgaacaat ggttcaccat gaacgacctc
     6241 gacctgatga ccgaaatcga aaaactcgcg cacgtgctgc gggtatctcc cgaccgcata
     6301 cagtatctca agcacctatc gctagacgat ttccggtcgc tgaacgactc gatctccaat
     6361 cgcctgctga ccgacagctc agatgtctgg tcaaagctcg cgggtgtcgc caagttcatg
     6421 cccaacttca tcaacgcgca ggttgccaag tcgctgctcg gtccgaacat taccgctaac
     6481 ctcacgtacc acatcgatac caaacaggca gtctctatcg ccagtagcct caacatcgaa
     6541 ttcctggcgc aggtcgcaga aaacctcgtg cccgcgcgcg ctaagcacct gatcgaagct
     6601 ttcccgatgg acgtgctcgt gaaactgacg cgtacgctga cgcgcgataa gaaatttttc
     6661 acgatgggag ctttcgtcga ttacatggat cgcgagaaaa tcctggctct ttcgcgcgaa
     6721 atccctgaca cagaatcact gctacgcgtc ggtctctaca cgcagaacaa ggagcatgtc
     6781 gcaaagctgg cagatggctt cagcgacgaa aaggtgatcg acatgattga aaccgcgcag
     6841 cagaaagact tgtggccttc gctgatcggc ctgctcgcgc acttcaccga aacacagcac
     6901 cgccgcctcg caggcctcgt gggccgcgtc tcggtcgatg cgctgctcgg tatggtgcct
     6961 tttgccattg aacacggcgc actcgtgcac atcttcgcct ttttgcagta cctcaacccg
     7021 gatatgcacg cacacatggc gacgatcgtc gcgaagctcg actctgaagc cctgtcgcgc
     7081 atcattcacg cagccgacga atcgggtaag ctgcctgtag cgctgaagat tgccgaccac
     7141 ctggcagagc gcgacgttga ccgcctcgcc gcgatgtcgc accgcttcga cgacgagctg
     7201 ctcaagcgcg tgatcgtgac agcgcatgcc gaaggcctgg taccgttcgg cctgcgcctg
     7261 ctggcgctat tgcccgaagg tgaaattcct cgcgcagaaa agatcgcccg tgaaatcgat
     7321 cgtaaagtca tcaccgacgc gcaggcacag atcgagcaga gcctgctgaa agaacggctc
     7381 ggatggttga agaagattta agtcttctgc ctgctttcag tcagacttgt cgggcgcctg
     7441 cacatatgga ttggcgcttt tttcgatcag cgcccactcc gcgcgcagcg cctcggcgtt
     7501 ggccttcgca gaaattgctg cctgaccaag caggcatgcc cgcgaaacag ggttttgttt
     7561 ctcccatggc tgcctgacgc tccccccaag cttcgcacag gtctttagtg actgcttaag
     7621 ccaggcttca aatgctgctg acgcaagcgg cgtaacgagc ctggtcgaca gccggaaagg
     7681 gttcactcgg ccgcttgatg gcaattcgcc acgctaccca ctggcggcaa ctcggtaaga
     7741 aattccttac acgtaaaccc ggttttggag tgaagcggtt cgacaaagat cagagcaaga
     7801 gatggggtct atcgtctttc aagcggtctt tttcggcctg gcttgttgcc acacgccgcc
     7861 tatttttcca gcaaatagag cgacgcaacc tcagccgcgg tgaggcctcg attgtagaat
     7921 cgcacttcat cgatgcggcc gaaacaattt tcgccgctgg ccgtgccacc aattgcctgt
     7981 acagtaccag tcgaaagcgt acctgcgaaa ttgtaagcgg agttaccgat ttccgcgccg
     8041 tctttataga tgcggaaaaa cgctccgtca taggtgccca caatatgatg ccaattgccg
     8101 ccggtataat cagcagcatt aatcgccgca ttcacattat gatagctaaa accgggaaga
     8161 ttcaattgga acgctgccac ggtcggcgta agggtaagcg caattgtagc gctgtcatcg
     8221 cgcgcgatcg atgaaaatgc accgatcgac gtaaagttta tccaggcaga gactgaaacc
     8281 ccggtcgaat tgaaattgat cggcggcact tcgatgcgcg tcgtcccgct aaaattataa
     8341 gagcggttag aattgccgaa gcgatcgttg gcaagggtgg cagagttgaa agttggatta
     8401 taagtcccct gcgtgtcatt ggcatttcca tcaaacttgt aatgggcgag caggttgctc
     8461 atcacgtcac ccgacgtgta ggtagctgta ttgacagtgt cgatggttct gccggcaaga
     8521 tcggtagcga cattcggcac tgccgtcaat gtgtagggcg ttgcagctga ttgcgtggtc
     8581 gtcgtgagcg taaacttctg gcaattcaca ccggcgccgc aggtactcaa caccgaattg
     8641 acgctgatcg ctcctgtcag gctgaacttt cccacgcatg cagcagcggc gttcggcaaa
     8701 tgcggcaaga tacccagtgg ggcataaagc aggacataat cagctataca agcaggtttt
     8761 ggctgcgcta ggttcatgag cattcctctc acccctatat tacttgacca agcatgaggc
     8821 tcattagtgt gcaaagatgc gtcatgtatt tctatctttc ttcctgctcg gcactatacc
     8881 ccccgtagtt gcgcaggaaa agccggctgc tcctggtaaa aaaatctttt ccatcaacat
     8941 aactccgccg cctgacacga gcgcgaccga gctggagttt tctgaaattg atctcaccaa
     9001 ggcgacaccc gagcaggcta cacaagcacg caccaaagtg atcaggacaa cagaaaaggc
     9061 ctaccgcgaa tatgttgcac ccgagttgcg tttcttccgc gtgcgctcga ttcacaaaac
     9121 cggcgtgccg ggtgcctttg gcaaaacgct gctcgttcag gactacctgc gcaaacctta
     9181 ccgtgagccg cagctgccgc tcgtgcaaca gggcaagaca gagtacctgc tcggcagccg
     9241 cattgagctg ccacagcaac caggcctggt aacgcgctac cggctcgatg agggcgagag
     9301 cctcgaatat aacgagccgc ttgttttcaa taagccgggt atctaccgca tgcaggtcag
     9361 catcgaaaac gaagcaaaag aagccgtgct cgcccgtacc tatatcttca aggtcgagct
     9421 caatgcaccg accacgcgcg ccgtgatcag cgacccggtg cacggcaaac gcggcatcaa
     9481 actgggtaaa gacagtgcgc tgctttttct cgcgaccgat gcggagtcag gcgtcgcaaa
     9541 aactttttac cggctggcgc cgatcggtaa gaatattgaa gagatgccgt ttaaagaata
     9601 cggcaaacgg ctcagctttg gcgaagtttc agcaggcgga gcgataaacc tgctgcagta
     9661 ttactccgtc gacgtggcag gtaataaaga agaacttcgc agtgaagtgg tcttttgcga
     9721 aaccgcgtcc gagtcagccc ccgctacgaa ggatacttct caataatggc tggtttctgc
     9781 tgcatctgcg gctgaaacca gagccctaaa cccagggcaa agaagaggac cagtacagga
     9841 ctcaaaatcg cgaagatcag ccagaagggg aaactcagca ctaccatgcg ccagcgcccc
     9901 gggttcacga caagcggcaa gaactttgcc gcagccgcag attattgcag gtattgcgcc
     9961 ttcagtcgcc cctgttcgcg gcgggcgtgg tcggcgccca aacccagttt ttctgcagtg
    10021 gcataaacgt ccatcgcgcc cgcgatgtcg tggcgcccct cgagaaagcg cgcgtattgt
    10081 gagtagaact gcgcgagcgg caggctgttt cggccggctt cgataatggc aagctcccgc
    10141 ttctgttttt caaattctgc ttcgagtttg agatacacgt tcagatcgcg gctgcgcacg
    10201 tcgtcgaaca ggctcttctt cgccgtctgc atttcctggt aggtcttcgc gttgcgcgcg
    10261 cgcaggtcag tgatcagcgt ttcgaggtcg gcgtgcgcgg cagcggcggc ctgcatgctg
    10321 ctgagcacct tttcctgcgc atttttcttc gaatgcatgt gcagcaggta ccggttggca
    10381 gtaaaacgct ctttgatcca ctgcgtgcgg tcggccacgt ctgtcgggtt cagtgccgca
    10441 atctctttca ggtaggcttc gaaaagttct gtcgctcgct cgtagtttcc cgtcttctcg
    10501 acgtgaagct tagccagctg ccagccgagg ttctgcttct ccttgttttc ggtggttgtc
    10561 gagtaggcct tttcgtagaa gaaagcggcg tcgacaaaac ggctcttcga atagtagagc
    10621 gcgcccagtg ccgcgttgga tttgcgcacc tgctcttctt tcagttcaaa acctgtgcgt
    10681 ggtttgtcgt ttgcagcaag cgccctttcg tatgaatcaa tactgcgcga tacatcctga
    10741 gaggtgcgat attcttctgc gagcgtaaaa ttggtcaccg ggttttccgg gtcaagccgc
    10801 gtcgccgcct caagaatatt ggcgtaagca gtaaactgcc ggttctggct gtagtcatgc
    10861 tgcagcgtct ggttgaaatt ggttttatag agcgctttct tattcaatac ctttgatcgt
    10921 tctttgattg catcttcaag ctggcgcacc aggcccgcca tttcgatcag catgtctgct
    10981 gacttgcggt tatattccct cgcatgctcg gtatatttct gctgcgcctg ctgaaaaccc
    11041 gccttcgctt tgtcgtattc gccgcgcacg cgctcgagcg cctgccggct cttttctttc
    11101 tggtcggcaa tcagctgttg ctgtttggca cgttcagctt cgacattcgt tgcggtggtc
    11161 gcccggccca gcgccggcga gcggctgcgg tcgtcgagca gcacaagctc ttcttcgagc
    11221 cgctgcttct cgcgctcttt ttcaaaaagc tgcttcttgg tttcgcggta gctgtctgcg
    11281 tcggtaattt cggcggcacc ggccttcacc agctgcaggc ggtctgtgtt ggtatagatc
    11341 tcgtcatcca tgcgcaggct gcggtagcgc agcgactgga caaaatgaaa tgccgcgtgg
    11401 tatggtttct tcagcttcat ctgcgcgagc gcatagttgt agtgcagcaa tgcgatattg
    11461 ggagcttctt tgataataaa agacggctgc gttttggcga gcaggtcacg catgaagagg
    11521 cgctgcaggt tgacgttcca ctctttacgg taccaggccg cgttcgagtc gttgtcctgc
    11581 cggttggcga tctttttttc ggaagggtca ctcaggtaaa actggttgag tttctgcccg
    11641 aggtcttcgg cgacgaggcg cgggttgaag ttgatcgaac cggcagaggc ctcgatttcg
    11701 tcgatgctgt aaggcaggta gcgttcgagc cgcttctgca ccgggttttc acgaatttct
    11761 ttcgcgtagc tactcccgag aataaggaga cataatgcag aaacggtcaa caggcgtagc
    11821 aaaagcgtaa atccccttgt tctgattatc ggcgaaattg cgaaagcgat tagcaaagga
    11881 gcagagtgtc gaaagattcc cgtgctcgct gcagctgcgc tcggtttcga caccctgcct
    11941 gaaagctgtt gtttaacagc tttctagaac ttcaggccca tctgccccgt gagccggcag
    12001 cgttcttgct cgcgctcttc gcggaaagcg gcgtaaacct cgtggtaaca ggtgccgcaa
    12061 ccggtcgttg catgggtctt ctcaatcagc ttcggaaacg tgtctgcccc ggcgcgtacc
    12121 gccgcctgga tatcagcgcg cgacacaccc ttgcagagac aaagttttcg cggacgcatc
    12181 aggcacgaaa ggtccatctg cgttaagggt attatagcac cggcagggcg gcaagtataa
    12241 atgggttatg ctttgccatg tgggagctat atgcaatacg agaccatgct cctgtttgtt
    12301 cggtatcccg ccgctgcccg ttacgctttg gtacacactg cttgcacact gttcttgctt
    12361 acggcttgtg caagccccga gatggtttcc gcttcagacg agagcacaca gcgctcaccg
    12421 gtgtcacccg cagaggccaa acggcgcgct ctgcctcact tgcctgctgt gttcaaactg
    12481 cgctgtgaaa aacgtatgga caaaagctgg tgcacgaaac ccgcgcgcga tcacgtgctg
    12541 atcgtcggcg aatattacca cgtcacgcgg gaaagttatc cctataaaac tctgcaggca
    12601 tatgttgagc cggcggtgcg tattcaccgc caaaccggtg agctactttt gcccgaaggg
    12661 ctgcgcgcta cttcttccga ttgattgact cgctggccgg caggccaccg atctcgagca
    12721 ggccctggaa gcggtcgaca ccgcctttct caggatactg cgcatacatc acgacgtcag
    12781 acaggttcac tgatgccagc gacgcgacgt catggttgag cgcaaccctg acaccggtct
    12841 gtgttttggt atagttactg aaaactgccc gcgcgccttt aatcggcaga acagataccc
    12901 acatgcgcat ctcggcgatc ttgccttctt cgtcgagcgt gaacaggtac gaatcgccag
    12961 gcgtgctacc gcccgatttg aaagacacga gcaactggtt atcaccgacc aaagcgcgtt
    13021 cagtacccgg tgaactgatg tggaaagcag ggttcagcca gaaggtatgg ttgtagaaac
    13081 gtttgatggc ggtttcggtc atgtctgcac agtcgtcggt aaccctgcgt tcttcctcaa
    13141 agcatactga cttgcctgat acctcgctga actgcacaag tttcttcttg ataataacct
    13201 cggtgagctt gcgcttacgg tcccagaaaa ttttgtcacc gccgcgaaag gtaaaagaga
    13261 cggcggcggt cgcctgccac ttatcgacat cggcgcccgc cagcattttc tgcgccagct
    13321 cttcagcacg cggccccggt gtgcccttcg gcaggctgct gcaggctgag aggaaaatcc
    13381 ccccgaaaag aaggatggta aggcgaagtg aacgcatagc taaataccgg gcagccagat
    13441 gcaccggcaa ctacaactgc gggcaaagtt acccgggggt acgaaaaatc agcttacaac
    13501 gtcgcggtgc ttgtacaatg aaccatgcgc atacaagact ggctgaagct gtcggcgctc
    13561 gtcgcaacgg caggcttcgt caagtggagc gcggtggtcg aatcggcatg gctctaccgc
    13621 gtgttcttgt acgtacctgc tgctctgctt tcggtctttg gttacggtga ctacggcgaa
    13681 tcgggcgggg tctatacctt tgaaacgttt gtgctcgact acagctgcgc gggagtgaat
    13741 ttttttctca tcacgctggc cgtcggttgc ctggcgatgc tgagtgcagg gcggttctat
    13801 ctcatcatga ttcctattgc ctggttcatg accctggcag caaatacgct gagagtcggg
    13861 ctctacctca agcttgagtt attggccggt caccggccct ggctgcacga ggccacggga
    13921 attctggtct ttttgacgct gctcgtcgtc tattatcatc tgttgatgag gagattacat
    13981 gaagaacaat aaccgccggg acagtggtct cctggcgctg gccgcactgg tgccggccgc
    14041 tatcagcctc gccatcgcgg tgcctatgct gtggatcatc tggcccgacc tcagcccccg
    14101 gcagatcgac agtgtgattt ctttgctcct gctaacgcac gggcttgcct ttggcatggg
    14161 gctcatcagc gcctggctct ggttgcggcg caaagatttg ccgatcagtc tgctcatcgt
    14221 cactgcacct gcgcagtttg cggcgtttta tattcaactg cgctggctca atgtgccgcc
    14281 gccgcgcggt tggatgttcg atgaagatgc attcatgacc ctcgtgctga gcggaatcat
    14341 tcctttgttt tacacctccc ttctgttgct cgcgacgcgg ttctctatcc ataatcgccg
    14401 tggtctcttc atctctgcag cgggaagcat cggcataccg ttgtgtatct acgcgctgtt
    14461 ccagctcatg cgctatgccc gtggcggttc aggtgcagat gcaatgttga ttgtcgttct
    14521 ggcttcgttg actatcgctt tttcttttct cgtactccgc ctgttacttt ttttgatcca
    14581 gcgtagggct gggtggctga cgactacaag ggggcgtttt tcacttcgcc tcacgttcat
    14641 cggccttttg cctctgcttg gcctgctcat gaacacgatc gggcctgtgg cgcgcgaaag
    14701 caggacggtt ctgggcgact tcactggttt acccttctgg ctactcgcgg tggtgaatgc
    14761 tctggtctac ctgttaccgc tgggccgcaa tctctttgcc aacgcactgc tggtggtgct
    14821 gcgcggtgtc gggctgtgct tcgtggtcta tttctgcgtg gtctttttgc tatacctgcc
    14881 cctcgcgttg ctactcatcg ctgcgctggg cctgggattg ctacttctga ttccgtactt
    14941 taccctcggt atacagtgga ttatcctcag ggaagattat acacagtgga gggaaaactc
    15001 ccgcgcgtgg cttttccctg tgctgctggc gggcggcttt ctgctgctgc cctgcacgct
    15061 actggccgac atgctgctcc agcgtagtca gctaatcgct gcggtggcct acgtacaaca
    15121 tcccccactc acactaaatc atgatgcaga cttcaaccgc tccagaatag aaagcctgct
    15181 cgctgaaccg cgcaggcagt cccggggttg gctcgaccag cgggaatcga ttccgctcta
    15241 cgacactctt cgccagcagg tcgtctatga cggcgtgggg cttgctgcgg gcatgcgcca
    15301 gcggttgcgc cacatattcc tgggagagga aaacgcagag accagtgcga gcaatacctt
    15361 gcccgaaggc cgggtcggcg cagtaactct atcggcgcgg caggacggta acgtcaccta
    15421 caccgacctg cgcattcaac ttgagaatcc tgacgcgaga cgcgaggcgg aactcgatgt
    15481 ccgggtcgcg ttgcccgaca acgcctttct cgccgggcat tggctggtga ttggccaggt
    15541 tgaacagccc gcgcagatca cctcgcgcag cgccgccatc tggacataca accgtatcac
    15601 cgaacagcgc cgcgacccga gtctcatctg gtacgaaagc aagaaccgtt tgcgctggcg
    15661 gctttttccg attccggcaa atggcaggcg cgaggcaagg ctacagattg cgcacttagg
    15721 gcaagccgac ctgcgcctgg gttctcaatc cgtacgactg catggtaaag aaaagactgc
    15781 cgaaaccaat gcagtgttgc ctgcacaagc acccaggcca tacctgcaat tcatcgtgga
    15841 ctgcggcaac aaaggcatgc gcgattactc cagcctggtg caggcagttg cacaggccac
    15901 gcgcattcca ctctctggcg ccaggataag tttcgtcaac gcatcgatac ggacaatcga
    15961 tctgcaatcg gtctctgacg cccggtgcga acctgcgcgt gaaggttttt ttgccgacct
    16021 tgcttttcgc gcgctgatgg cagaaaagaa tcgcgccgca tcttctggct ttcccgtctt
    16081 tgtggtgctg agcgcgacac tgcccggggc taattggagc gacatctcgt acttcacgcg
    16141 cgactaccgg gacatggatt tttttatcca tgcagacggt aagggattgc acgcttatga
    16201 tttttacggc aatcgcacaa gactgccggg cttcagcaac ggcgtaacct cgcagcgcag
    16261 gcaaagggcg caacctgccg gtatggaaaa atttctgcac ggccaggaat tatacgaacg
    16321 gtggctcagg ggtgaaaacc cggcccgcca tgaggcgata cgaacggcga tgcacacggg
    16381 tatcctgaac ccggccgcag catcgattgt gctcgagacc gaggcacagc gtcgtaagct
    16441 ggccgaaatg aaccagcgta cgctcaaaag ccagcatgaa ctggaaaccg gcaacagaat
    16501 gcgtatgtct gaacccacta ctgctctgct gctcctgttg ctgctgcctt tcctggtccg
    16561 gcgcctgggt ggaggtaatt ctttaaaaaa aaatgatcta tcaggataat ttttttcaga
    16621 aagagtgaga aaataacgca acttccgggt atttaatata tagagggtca aaagcccggg
    16681 aggaaatttg tcactggtaa atatcacttt tgcgctctct gctccagcaa aacgcgcatg
    16741 ggagaatctc agtaatcacc gccatttcga ccaggtggcc gaactgtttt tcgacgccat
    16801 gcgccacgaa aggtaccgga acttcgtggt aaaaccgcat ttcttcacag aagtacacgg
    16861 ccaccgcagg gcccggaaac attcgctgcg atcacccggc gaaaaactcc acagggtcag
    16921 gctggtgatg cagctgcaga ctctgcaaaa aatgaaacgc ctcgagaacc tggggctcgc
    16981 actgtcatgg caggtagaag aaattttgta cttcggtttt tgccgcggat tgttaccgag
    17041 aatgaatcca tattattccc tcgaaactaa tgacacttgt gtcacaaaat aactcgacgg
    17101 cctgttcagg ttccgtgatt gtgctccatg caggtacaac tcgaaaaagc cagggcatcg
    17161 gccaacttca cgcggtttcg cggtgaaaag agcggcgggc actatgaaag ttacttcatg
    17221 cgcgccaaca atgccgagac cggccaggcc ttctggatcc ggtacacaat ctaccagaac
    17281 gagacaggca aaggcacgaa gtcacccatg ggcgaactct gggcgatctg gtttgaaaaa
    17341 ggaaaagcac cggtagccgc aaagtcggag ttcccgctca gcaaagcaga atattccaac
    17401 ctgcagtttt cactcaattt tgacggggca acgctcgatg ccaacggcct caagggcgcc
    17461 gcaaacaatt gtggtaccaa tgaaatcagc tgggacctgc aaatagcccc gatgagcaag
    17521 gtacgtaacc tgacgccgct ttttcccatg ccactggcat cttatgacac tccgctgcca
    17581 cgcgcaaagc tgctggtcgc tcagccattc gtcacgttta aaggacaact ggcagtcaat
    17641 ggcaagaaga tcgccataca ggattggcaa ggcagccaga accacaactg gggctcgcgg
    17701 cataccgatg aatacgcgtg gggtcaggta gccggttttg acaacgaacc agatgcgttc
    17761 ctcgaagtcg cctctgccaa actgaaattt ggtcccctca tgactccctt cctgacaccg
    17821 atggtactgc gtttccacgg caaagactat tgcctcaaca cgtactggca ggctattcgc
    17881 aaccaggcag atcttgaata cttccgctgg tctttttccg gggaaagcaa agacctgaaa
    17941 attgaaggta agatctctgc caaccgcgaa gatttcgtcg cgctgaacta ttataacccg
    18001 ccgggagacg tgaagacctg catcaacagc aagattgcat cgtgtgaagt cacgctgaca
    18061 gagaaaagcc gcatcggcgt cagcacccgc ctggtgacca agaaccgggc ggcctttgag
    18121 atactgacca gcaagacaga tcacggcttc accccgttgt tctaaccaac cacaaaagag
    18181 attgaacctg taagcgggtt cataagcata acacatggtt ataaaattaa ttgtgatgtt
    18241 tgcgctcttt tgcgctctac tctatttgct caacaaggcg atgcccgatt tccacatcga
    18301 aatgggcgcg atacccgtgg tcgccctgat actcgtagga gtgaacgtgg tggttggcgc
    18361 gctgctcggc ggtacggcaa ccgtactgaa tgtgctcacg ctcggtatta taaagtggat
    18421 tctgcagttt atcactctgg gtctttttgg gcttctcgtc agcttcgtct tcaatgtcat
    18481 catcttctgg gtggctgaca aactgacaga tcgcctgacc attaagacca tgcgaacgct
    18541 gctcgtaagc gcaggtgcac tgcagtttac caacttcctg ctcaacaggg ttttttgatg
    18601 aaatatattg tttgtctgac tttgctcctc gcaagtgcag tgggagctca gactgcccag
    18661 aaaggcaaaa agacggaatg ccggctgtcc cgctaccagc cgcaggcgcc tgcgttggcc
    18721 gacctaaacc tggaagaagc agaaaagcgg gaaattgtgc agctcataca ggaaggccga
    18781 aagattgatg cagtgctcat catgcgcaag tgccgcgaca tcacgctgaa agaacagaag
    18841 atgatcgtcg acgcagtaca gaagaacttc tgatacctgc tgaaatccag gttacgcagg
    18901 ttgatcgcgg cgcggcgcag caggtttttg aactagaccg cgagattttt ggcgaaggct
    18961 gctggtctca ccagcagtgg gtctctgaat tcgcctcacc cggtgtcatt atcctgctgg
    19021 ctgttcaggc aggacagcct tgcggtttcc tgagttcggg catagcaggc gatgacctgg
    19081 aaattcgcaa ggtcggtgtc ctgccgcggt accgccggca tgggctgggc cgccggttac
    19141 tacaacaagc cacgatgaac acagcaaaca ccgccaggcg ctgcctgatc gaggtctcat
    19201 cggccaacgc cgaggggctg gctttttaca gacggacagg cttcacggaa attggccaac
    19261 ggacaagtta ttatgcagac ggcagtaccg ccatcgtaat ggagaaaatt ctgtccgtgc
    19321 cataggcaaa accttatatt accgccatga ttcgtaatcc gtttgatacg ctaggctcga
    19381 ttgttgccga tgatgaagag ggcaccaaga aaaaagaaga acgtccacca gcgctgcgcg
    19441 agcgcatcgc cgagcgcttt ctcgacaaac ggcagatttt cctttggggc gccgtgacag
    19501 ataaatcggc tgaaatgatc gtcgaacgga tgctgttcct tgaagccaca gatccgggta
    19561 agccaatcta tttcttcatt aactctcccg gcggagtcat cacttcgggc atggctatct
    19621 acgacgtgat gcgcatgatc tcatcgcctg tatacactat caccatgggt atggcagcat
    19681 ccatgggcgc cattctgctc acggtcggcg aaaaaggcca ccgctacgtg ttcgaacatg
    19741 ccaaggtcat gatccaccag ccgctcatca gcgggcagat cgtcgccccg gcgatcgaca
    19801 tcaagattca tgcagaagaa atcaagaaaa cgcgtgaaga gctcaatcgc atcattgccg
    19861 atacgacagg ccagccactg acgcgcgttg aaaaagatac cgaccgcgac tactacctgg
    19921 atggcaaagg cgcagtggaa tatggtctgg ccgacaaagt acttaccgac ttttcagaac
    19981 tgggcctgtt cgacacgaag ccggcaaaag cagcaaaacc caaaaagaca aaatagtcag
    20041 tgactttttt ctagcccagc tgtttcatca gc
//
LOCUS       DAOQJF010000009        19593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1261_length_19593_cov_5.834272, whole genome shotgun sequence.
ACCESSION   DAOQJF010000009 DAOQJF010000000
VERSION     DAOQJF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 19593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19593
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_19593_cov_5.834272"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..567)
                     /locus_tag="PLF85_01035"
     CDS             complement(109..567)
                     /locus_tag="PLF85_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNE18266.1"
                     /translation="MEYLNNFLYWVIGGAVLVLLEFLVPGLVVVFLGLGALLTGGVLY
                     LGWIKEPVALLAFFGFSSILMLATLRRFVMRFYPSDSERVEADEDKLLIGQKATVIST
                     VYPSDFSGRIRFSGTTWSARSEGGEITEGAEVVIVGRENINFLVRADRIH"
     gene            704..895
                     /locus_tag="PLF85_01040"
     CDS             704..895
                     /locus_tag="PLF85_01040"
                     /inference="COORDINATES: protein motif:HMM:NF037012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18267.1"
                     /translation="MTRVILAYDNSGIYLFVIHGSISVLGETLRPRDAIGITETNSLT
                     VEALEGSRVLAIEVPGWGD"
     gene            947..1375
                     /locus_tag="PLF85_01045"
     CDS             947..1375
                     /locus_tag="PLF85_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE18268.1"
                     /translation="MSSASQDSSPARRLANDRPAQILIVDDNDSNILILERMLQKLGY
                     TPQSTDNGRQAFSFITGKKYDLILMDIMMPGIDGLELTRAIRNAESAQNPIIVAVTAD
                     VTAQNRDACFLAGVDDFLPKPVDFQALTAMIESWVPRANP"
     gene            complement(1435..2586)
                     /locus_tag="PLF85_01050"
     CDS             complement(1435..2586)
                     /locus_tag="PLF85_01050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE18269.1"
                     /translation="MSDPLAQKLRVAWIADIFDEVSGIITDTEEMYQLAQERGYFWQP
                     VTTYKKPIHPFHIFDPILPIPTGSFYKGTSMYVPDFLRVVQFLRANKFNVLVSNTPGV
                     MGMLAMAAAKYLSLPWVDIYHTDIDYYMNDLSGRLVKPFVKNPALFFLKQYQKQADLI
                     FVRTREFYDLMVRKGHDERKLRYYPAGVNARHWNPENADRSVFNEHGIDPEATVVIFV
                     GRITKVKDIEFLLRYFTEQKPHKAVCAVIGGGPEKELYEKKYAGENVRFLGVQRGAAL
                     QKLYASADLYVLPSASETLGKTVLEAMASGTGVVVSDKGGPKDYVTHGENGLVFSAHN
                     YESFSAAMDSLMNNRELIRKLGSVARASIGNHTDEKLFESFTQHIAALV"
     gene            2687..3190
                     /locus_tag="PLF85_01055"
     CDS             2687..3190
                     /locus_tag="PLF85_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNE18270.1"
                     /translation="MRFDDAHRPWRDTLSEDCIHLVTPGNPGRLDVSDKWTLISSLHR
                     TLCERERIQAGLCSEIEKELIERERLMSTGIGEQMAIPHAVVAEADRFMTECAIIREG
                     IEFESIDNTKAYIVVMLVAPKSALQGHLKVMAEIARVFYRAETRQKVIAAANAAEVLE
                     VVRNAPV"
     gene            3197..4351
                     /locus_tag="PLF85_01060"
     CDS             3197..4351
                     /locus_tag="PLF85_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE18271.1"
                     /translation="MNIFQALRGEWGLTRDADFRRDPWYIVSGKQVVDSGFGKPPKEF
                     FTKTLPPGVVVPGLVNAHSHIELSCMAGKVPVGTPLMGMGSIIHTQRGQMDSRQIRQA
                     AVDAIRAAEDEGTFFFCDICNSTEFIRFLSDLPTFNGNRYLELLGFSSPSDQQRMDAA
                     RALLDIDATFYPTVHSVYGSSPLQMHFVREKARHTSVSIHLLESPEEIEFQHDRGPIV
                     EFLKRLGQHVKHPELQGRSAVEYLHSVGMLSFKKLLLVHLSAARVEDIDLLSEYVPHA
                     AWVICHRSNKYLGIERTNWETLRHSPLKMLIGTDSLASNTDLSVMREMKAILSEGKYS
                     ERELMRAATWDAYEFLEIKDNRVPHFLFPGAQPNILSMSAAGRAIPLADF"
     gene            complement(4393..5820)
                     /locus_tag="PLF85_01065"
     CDS             complement(4393..5820)
                     /locus_tag="PLF85_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNE18272.1"
                     /translation="MKYRALLLFFGLPVALAAQVNSAGTGGRVSWPQEYVMEILRPAV
                     TGGEAKNYLSGVVQNLIADQLTLQRSAVTRGRLSPLTIEYSLQPDHDALPQGEREQLL
                     SRYPKPDILPLRIRRELRVSSPEAECTGLRDDRYTLLTAITASANRVQVRISLCQGSD
                     LLQSQRTNADEQELVAAVQRLVNPVRAKLTGDNFATLRIESNPPQASVYLDDQFLGKT
                     PLDYSYLIPGSYQLVLKLDGYRTKSEPLKPQTGQHVQRLLALDASPAGGSIDINTAPP
                     GAKIYLDADYYGKTPKRLENISAGTYRLHLLHPEKGEVYRSVTLNEKNGTLKIDENLN
                     GFLADRQPGLMGLGYKTWYQMSLVTSAAFFGAGIAFYVWRDSAQEQIFGRLSGKATSL
                     YTQEDRDFVAARNSEYDTRGNYATAFMVTSGAFAVLSLYFYVQHLLSQDEGIVMKPRQ
                     PVAGEVEYRVGGLHGPGEFSANYRF"
     gene            complement(5817..6227)
                     /locus_tag="PLF85_01070"
     CDS             complement(5817..6227)
                     /locus_tag="PLF85_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNE18273.1"
                     /translation="MSEIESREAELLADFEMFDDWADRYRYLIELGEKLPALEDAHKV
                     EANKVRGCQSQVWLIARREGDRVFFEADSDSAITRGLIALLVRLFSGAGSAEIAAANL
                     SFIEKLGFAKHLSMSRANGFASMIQTIKRLAAQP"
     gene            complement(6239..9358)
                     /locus_tag="PLF85_01075"
     CDS             complement(6239..9358)
                     /locus_tag="PLF85_01075"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE18274.1"
                     /translation="MNFFQSVYLDYFTVGSVIPVLFLFICAAVFFQVRDKSRATRVVA
                     SALLIYGIFVSSYVVASTFYHPLAAFHRWTTVGFVLLAVTLLTQIFFEFPEPVHTRVA
                     KILARVMTVIALAFAAWFMVQTYDAGYVYHFEGHYYDFDADDASYVISIAILVYVGIL
                     IGIGVWRVIAVTGRDRWALVGLIVGIVAATILPAYLNTLSREGGMDRGTFQTVFDMST
                     ILGLFVIVIIYMNNTADKTTFMAKIILVSVVVFLLILQAISYFSFQDKETAYDGVKSE
                     QANRYRETGLEPEAMRYAIGYDLVKKKTEPMASISGAEIDTEEQINGFEFSNIWMKIF
                     YLPEDIAFKDNVEKILQSAPPTFAGYKAALLPLLEKKDTNNKQAILQKIGSLQRPVVH
                     QRYKLQKIPDFQFREKATKFLGKTRGQFATFSDAMLVYLKENATDNGAELKKKLLRYA
                     EEIIPPGARHYRTDKKSGAHYTAYVGIDYKKNRLVEVGFDYLDYRKYVHPLGLKYLLL
                     IFAAIVIIIVGFPVFFSRALVTPLNSLLSGVNQVNNGNLDVALPIYVEDEIGYLSRSF
                     NGMVHSIKDSKQKLQEYAETLEDKVKERTAELQKAFEEVNALKKQQDGDYFLTSLLTN
                     PLGSNKANSPNLNVDVIVEQKKKFEFRKWHRDIGGDTVITSTITLKDKPHTVFLNADA
                     MGKSMQGAGGILVLGSVFQSVIERTKLSPKEQDVFPEHWLKYTFVELHRVFEVFDGSM
                     LISLVMGLVDDESGLLYYINAEHPWSVLYRKGKADFIEKEMALRKLGTQGVSGHIEVQ
                     TLQMEPKDVIIIGSDGRDDLLVGYDEDGARIINENEQQFLKIVEESEGQLDRIRDVLE
                     NYGELTDDLSLLRIGYKEDVHHDHGQLSQHVIQLLNRARLAVKESNLKVAIGDLEEAL
                     RIDDTQPEVMRDLIKAYLKDKQFEKAAALAEDYIHIKPADSEFLYIASYCHKVLQNYK
                     KAADFGERLRLRDHDMVKNLINLADIYYLQGLYNRSRMIIEFAEKLDPHNIKVRRVLE
                     ALAKKGPTA"
     gene            complement(9430..9927)
                     /locus_tag="PLF85_01080"
     CDS             complement(9430..9927)
                     /locus_tag="PLF85_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18275.1"
                     /translation="MQPVKVIHLIKRVEQIDKEIAELHGLVDGIESTRSYRNTLKIAA
                     EQQINNLIGEKVKLMELKIENPPDFLTKHVFESTENKMPQPRISLEALQGGVSTTYSG
                     PAAEVDEMPTGAKNPLRSYTRMMSVEEILPKAGSESATRAKRAEKPPVKTRADILRDL
                     PPLQY"
     gene            10139..11893
                     /locus_tag="PLF85_01085"
     CDS             10139..11893
                     /locus_tag="PLF85_01085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE18276.1"
                     /translation="MLFTELKLAEPIQKRLAEKGYPEPTPIQAETIPLALNNRDLIAC
                     AKTGSGKTLAYLLPILQYVHESGLKQADAEQDGVNPPVALVLAPTRELVVQIAEEAEF
                     FAAAAGLKLATIYGGVDHDKQRRELSSGAHLIVATPGRLLDFIRGGEANLSKVVRIVL
                     DEADRMLDMGFIDDVRKILSKCSPNENEDRQFSLFSATINFSALYSVWEFMREPEEIL
                     INPELIDHANIAQEMLHLGQDEKLPYLIQLLENNKLEPVIIFTNSRQYVDVLVKNLNH
                     HNIAAAGLSSMVTQNKRLKILDDFKNGKFRVLVATDVASRGLHIEDIQLVVNYDIPMD
                     PETYVHRIGRTARAGKTGKAISVSSEFDYDSLAKLERYLKYKIPVAQPEARYLENVSF
                     VRIVHVERQDGADHVRGGDRRGARGAERGGRDRKRHEGGRGPRREERSGGEARSGEGR
                     KPHAKGAQGAPQHQKQKDGAYFDARRGRGGNEQRGRDGQRGQQAGQRRGRNDNFAIVP
                     RTPEKVGFFTRIGRSLKALFTRKPAKPQISERTRRLLEQETHGGGDKPRGNNKRRRRG
                     GNNRRGGGRERQGNRERR"
     gene            11899..13344
                     /gene="wbaP"
                     /locus_tag="PLF85_01090"
     CDS             11899..13344
                     /gene="wbaP"
                     /locus_tag="PLF85_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HNE18277.1"
                     /translation="MRALRKSLYPLSLVGIDLLAYTAAFVCAVNLRIWLGKTFPGIPD
                     FSGDAMRMNDLYFMPALLLVIFFYEGLYRRRMPFIDELREITKSLAILTVFLFALISI
                     GNLAHLVSRLTILLMAPLALVTIGIARYWGKLLLHRFGIGTQKLLIIGEVAAAAQIKA
                     ELLAEKTLGYRFAGFIPIDRREENLWHKQQAAGLTRAGSADEVEAILARREVECVLLA
                     TPSSSREKTSALADKVYRHVHHVLMIPEMGSGALLNSELYHLFVNQMFLIKVRNSLTE
                     RPARFSKMLFDMVLVVLSLPVIAPVLLVIGIMVRATSPGPALFRQVRIGKNGKEIRVF
                     KFRSMHRDAEAKLAELLAKDPAARAEWKKTHKLRHDPRVTKLGAFLRRTSLDELPQLI
                     NVLCGDMSLVGPRPVTETELKTKYKGKADYYKLVRPGITGLWQISGRSDISYEQRVNM
                     DSWYVFNWSLHIDLVILYKTIWVVLGRRGAY"
     gene            13427..14335
                     /locus_tag="PLF85_01095"
     CDS             13427..14335
                     /locus_tag="PLF85_01095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE18278.1"
                     /translation="MVIPVRNGGALYRQCLESIAAQSLKPAEVLVFDTESDDGSATLA
                     AAVLKKIPLRTFSVARSQFDHGGTRNRALQAARHEWVLFMTQDAICADTEAFAHLLAS
                     ASAQNVVAVYGRQLPHSDASPLAATARGFNYGTERVEQNMALAQKLGIKTWFCSNSFC
                     LWNKPALENAGGFAEKLILGEDMHAAARLVQAGGKVIYEPTAQVRHSHNYSAAEEFCR
                     YFDTGVFHSLHRELLFRAGNPSKEGLRFVLGQLRQLVKFRAWLSVLRLPFHVAAKFLG
                     YKLGQNYTLLGRRLSRALSMHKNFWN"
     gene            14404..15609
                     /locus_tag="PLF85_01100"
     CDS             14404..15609
                     /locus_tag="PLF85_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNE18279.1"
                     /translation="MGIGLAFSISIAQTGLGITFVIFIATLFSAGRKSLSLKGAHWLK
                     LLYLFFGGWVLWRIVHVLIAARPGAELFEAREVWLMLIPVFIWFYASSRNRLHTVLAA
                     FVLGAAVSSAWGIWNMRNEIFDMWTRGRGLSTMHHLNYAGVAAFAALLGLGLSWSYYY
                     SGKKFRAALILFLALLSFAGLWLTKSKGAIAAFAAVVPLFIYLQLHNRVHRWLFIALA
                     VAGAAYLVPRLPESIAEQYRFPPPEVHAGSQAERRDLWQTGLAMIHDNPWVGYGERGY
                     NEAYPRYQLPGATGVAQWDEKSREASHMHNDFINTAVLYGAIGLLLQLGYYFLGFFAY
                     LRERFHIRRQSDRPLAAAGASSLVLMALMGVTQCHFTSEIVQMSFWVALGSLFVLLDS
                     DRSGRGEET"
     gene            15692..16171
                     /locus_tag="PLF85_01105"
     CDS             15692..16171
                     /locus_tag="PLF85_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNE18280.1"
                     /translation="MFNIEENVVYPMHGVGKIVEIATKTILNKKKKYYVVELINTKMR
                     VMVPVDNAETIGLRHIMDKKQIPKIAKLLQEEYVEQEEDWKIRYQNNLTKVKSGSITA
                     VAEVCRNLYKRARDKELSVMERKLYESAYSLMINEVALAKGISAEDASNMISDILSK"
     gene            16530..17129
                     /locus_tag="PLF85_01110"
                     /pseudo
     CDS             16530..17129
                     /locus_tag="PLF85_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
     assembly_gap    17240..17339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17340..17570
                     /locus_tag="PLF85_01115"
     CDS             <17340..17570
                     /locus_tag="PLF85_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNE18281.1"
                     /translation="NYENRQELRRVQTPQLLHYPTFIAAQSAALGRGQMELDFTDEGS
                     VCLSLGLRVGSFEGDTENLKITFPEDLKPDKL"
     gene            17604..>19593
                     /locus_tag="PLF85_01120"
     CDS             17604..>19593
                     /locus_tag="PLF85_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE18282.1"
                     /translation="MKSILSGLAALPIVLLLANAGTQAQTGVSVTPDNEAQILNLGVI
                     NTPYSEYTPFITADERYLFFQSNRPDGVNNSGDFDLWYTENQVTEGVPKFKTPANVGL
                     PVNSAYFDGHPTLRRLPSGEYEMYFASFAEEGRPGPQLTNLYYTIFKDGKWTQPMPVV
                     EVNTDFHDRMPSISQDGRYLFFSSDRPGGFGRDDIWLSEYDFAAKRWGKPRNAGRTIN
                     TPASEVTPAIHSDNITLYFSSDRAGGVGGYDIYVTQMLQNLPSASMTNEGESMWKRPV
                     NLGKPYNSTFDDEYPTVIRNGNYMYFASNRENGQGSFDVYRGKVPEFAKPEVVVTLKG
                     RVKEKFSDKGIEANIRITDLDGEKNFSTNQPEGLYSADLINKKQYKLTVTAPGYQPIE
                     YIADLREIHTPITIQKDFEMERVLALPKDVVLRVTFENESGNSLKPNATYRLPPGVAD
                     DTILPYKGSVAQLKVPAMSRYSKPEEALAALDAMRLSVKASLKGYEDLAEERAMAQLL
                     RGSNGELKGIIDVKFVMKGKQEGVEVIPTKGPGGLEAIVYFSTNVSNRLNNEKASGLK
                     NVVDLWNKQPHKYVYVYGHADSRGTSAHNMKLSKARAEFVKRRLVEYGIPSEMIITRA
                     FGETRPVSKDESTLEGRRKNRRAEIHFDATKRKDEGEPT"
BASE COUNT         4517 a         5493 c         5282 g         4201 t
ORIGIN      
        1 ctaaacgcag ctcgtaatgt gtccgtcaaa caattcggac acagttccta tgtggactgc
       61 aggtacagcc gggcccgtaa agcagcggcg gcagaaattc agccccggct aatggatacg
      121 atcggctcgc acgaggaaat tgatgttctc gcggcccacg ataaccacct cggcgccttc
      181 ggttatttct ccgccttcgc tgcgtgccga ccacgttgtg cccgagaaac ggatacggcc
      241 ggaaaaatcg gaaggataaa cggtcgagat taccgtcgct ttctgcccga tcagtagttt
      301 atcttcgtct gcttcgacac gctcgctatc agacggataa aaacgcatca cgaaacggcg
      361 cagcgtcgcg agcataagga tagaactgaa cccaaaaaat gccagcaggg cgacgggttc
      421 cttgatccag ccgaggtaaa gaacgccacc cgtgagtagc gctcccaaac cgaggaacac
      481 cacgacgaga ccaggaacca ggaactcgag caataccagc accgcgccac caattaccca
      541 gtagagaaag ttgttgaggt attccatgcg cgcagtgact ttagttcggt cattcaacgt
      601 gtcagccgaa aaagtcccgg gaggctgctg aaaagggcat aacctgcaac catgaccggg
      661 gtgaatattc gccagcgcaa aatcttcgta ctattatagg tgcatgacca gagtcatact
      721 ggcttatgac aactcaggca tttacctctt cgtgattcac ggcagtattt cggtgttagg
      781 cgaaacactg cgaccgcgcg atgcgattgg tattaccgaa actaacagcc tgacggtcga
      841 ggcactcgaa ggcagccgcg tgctcgcgat cgaagtccct ggctggggtg actgacaccc
      901 attctgtttg acaaccagac aggtaatacg agcaaaatcg cacccgatgt cttctgcatc
      961 ccaggacagc agccccgccc gaaggctggc gaatgatcgc ccggcgcaaa tacttattgt
     1021 cgacgataat gattcaaata tacttatact cgagcgcatg ctgcagaagc tgggttatac
     1081 cccgcaatcg acggacaacg gcaggcaggc ctttagtttt atcacgggaa aaaaatacga
     1141 cctgattctc atggatatca tgatgccggg catagacggg cttgaattaa cccgcgcgat
     1201 tcgcaacgcc gagtcggcac aaaacccgat tatagtggcc gtcactgccg atgtaacggc
     1261 gcagaaccgc gatgcctgct ttcttgcagg agttgacgac ttcttaccaa agcctgtcga
     1321 cttccaggcg ctgaccgcga tgatcgaaag ctgggttccg cgcgctaacc catagggaat
     1381 tgctgaaaaa gttgcgtacc gcgctgaaca ggcacggacg ttgcgcaaaa accatcagac
     1441 cagcgccgcg atgtgttgcg tgaaagactc gaagagtttt tcgtctgtgt ggttgccgat
     1501 cgaagcccgc gcaacggacc cgagtttccg gatcaactcc cggttgttca tcaggctatc
     1561 cattgctgct gaaaatgact catagttgtg cgcgctaaag accagcccgt tttcgccgtg
     1621 cgtcacgtag tctttcgggc cacctttatc agaaaccact acacccgtac ccgaagccat
     1681 tgcctcgagt acagtcttgc cgagagtttc actggctgag ggcagtacgt agagatcggc
     1741 gcttgcataa agtttctgca acgctgctcc gcgctgcacg cctaaaaacc tcacattctc
     1801 gcctgcgtac tttttttcgt aaagttcttt ttccgggcca ccaccaatca cagcacaaac
     1861 tgccttgtga ggcttttgtt cggtaaagta acgcagcagg aattcaatat ccttcacctt
     1921 ggtaatccgc ccgacgaaaa tgaccaccgt cgcctcggga tcaataccgt gctcgttaaa
     1981 gaccgaccgg tctgcattct ccgggttcca gtggcgggca ttcacgccgg cggggtaata
     2041 acgcagcttg cgctcatcgt gccctttgcg caccatcagg tcatagaact cgcgggtgcg
     2101 cacaaaaatc aggtctgcct gcttctggta ttgcttgaga aagaacagcg ccggattctt
     2161 cacaaagggt ttcaccagcc ggcccgaaag gtcgttcatg taataatcga tatcggtgtg
     2221 gtagatgtct acccagggta gcgacaggta tttcgcggca gccatcgcca gcatgcccat
     2281 cacgcccggt gtgttggaga ccaatacgtt aaacttgttt gcacgcagga actgcacgac
     2341 gcgaagaaag tcgggcacgt acatactcgt gcccttgtag aagctgccgg tcggtattgg
     2401 cagtatcggg tcaaaaatat gaaacgggtg aatcggtttt ttgtacgtgg tgaccggctg
     2461 ccagaagtaa ccgcgctcct gcgccagctg gtacatttct tccgtgtctg taataattcc
     2521 cgacacctcg tcgaagatat cggcaatcca cgcgacacgc agtttttggg caagaggatc
     2581 gctcataagt cagcctgcct tgacacgccg caaacgcagc atgtcaagta gatgggattt
     2641 tcgattgtcc ttgaccgctt gatagtgagg cgacataatc ccccctgtgc ggttcgacga
     2701 tgctcaccga ccatggcgcg atacgctctc tgaagattgc atccacctgg tgactccggg
     2761 caatcccggc cggcttgacg tatccgacaa gtggaccctt atcagcagcc tgcacaggac
     2821 gctttgcgaa cgcgagcgca tacaggccgg gctttgctcc gagatcgaga aagaactcat
     2881 cgagcgggag cggctgatga gcacgggcat cggcgagcag atggcaatac ctcacgcggt
     2941 cgtcgcagaa gccgaccgtt tcatgaccga gtgcgccatt atccgcgaag gcattgaatt
     3001 cgagagtatc gataatacaa aagcttacat tgttgttatg ctggttgcgc caaaatctgc
     3061 gctgcagggg cacctgaaag tgatggccga aatcgcccgc gtattttacc gggcagaaac
     3121 ccggcaaaag gtaattgccg cggcgaacgc cgccgaagtc cttgaagtag tgcgcaatgc
     3181 accggtctga aattctatga acattttcca ggcattgcgg ggtgaatggg gtctgacacg
     3241 cgatgccgat tttcggcgcg acccctggta tatcgtctcg ggcaaacagg ttgtcgacag
     3301 cggtttcggt aagccgccga aagagttctt caccaaaacc ctgccgccag gcgtggtcgt
     3361 gccgggcctg gtcaatgcgc acagccatat cgagttgtcc tgcatggcgg gcaaggttcc
     3421 cgtaggcacg ccactgatgg gcatgggcag catcatccat acgcagcgcg ggcagatgga
     3481 tagccggcag atacgccagg cagcagtcga tgccatccgc gctgcagaag atgaaggaac
     3541 tttcttcttc tgcgacatct gcaacagtac cgagtttatc cgcttcctga gcgacctgcc
     3601 aacctttaac ggtaaccgtt acctcgagct gctgggtttt tcttcaccga gtgaccagca
     3661 gcgcatggat gccgcaaggg ccctgctcga tatcgatgca acattctacc cgacagtgca
     3721 ctctgtctac ggttcgtcgc cgttgcagat gcatttcgtg cgcgagaaag cgcgccatac
     3781 ctctgtttcg atacacctgc tcgagagtcc cgaagaaatc gaattccagc acgaccgtgg
     3841 cccgatcgtg gaatttctca agcggctggg ccagcatgtg aagcaccccg agctgcaggg
     3901 acgttcagcg gtggagtacc tccactcggt tggtatgctc tctttcaaga agctgctgct
     3961 cgtgcacctg tctgcagcgc gcgtcgaaga cattgacctg ctgagcgaat acgtaccgca
     4021 cgccgcctgg gtcatctgtc accgctcaaa taagtatctg ggtattgaac gcaccaactg
     4081 ggaaaccctg cgccattcac cgctcaaaat gctcatcggc acagactcgc tggcgtcgaa
     4141 caccgacctg tcggtaatgc gcgaaatgaa agcgatcctt tccgagggta aatattccga
     4201 gcgcgaactc atgcgcgctg caacctggga tgcttacgaa ttcctggaaa tcaaggacaa
     4261 ccgggtaccg cacttcttgt tcccgggcgc acaaccgaat attctttcca tgagtgcagc
     4321 cggtcgcgcg ataccgctgg ctgatttcta gcgggctcct gaaaaaggag ccagaagcag
     4381 tacataatgc tgtcagaacc ggtagttagc agaaaactcg cccggtccat gcaatccgcc
     4441 cacgcggtat tctacttcac cggccacggg ctgcctcggt ttcattacaa tgccttcgtc
     4501 ctgcgagagc aggtgctgta cgtagaagta gagcgatagc acagcaaaag ctcccgaggt
     4561 taccatgaag gcggtcgcgt agttgccgcg cgtgtcatat tcactattgc gcgctgcgac
     4621 gaaatcgcgg tcttcctgcg tatagaggct cgtcgctttg ccgctcaacc ggccgaaaat
     4681 ctgctcctga gccgagtcgc gccagacgta gaacgcgata ccggcgccga agaacgcggc
     4741 ggaagtcaca agcgacattt ggtaccaggt tttgtaaccc agccccataa gcccgggttg
     4801 ccggtcagca agaaagccat tcaggttctc gtcaattttc agcgtgccgt ttttttcgtt
     4861 gagcgtgaca gaacggtaaa cctccccctt ctcagggtgc agaagatgca ggcggtaagt
     4921 acctgcagaa atattctcga gtcgcttggg cgtttttccg tagtagtcgg cgtcgagata
     4981 aattttcgca cccgggggcg cggtatttat atcgatacta ccccctgctg gcgaagcgtc
     5041 gagcgccagc aggcgctgca catgctgacc ggtttgcggc ttcagaggct cgctttttgt
     5101 tcggtagccg tcgagcttga gaaccagctg gtaagaaccc ggaatcaggt aagagtaatc
     5161 gagcggggtc ttgccgagaa actggtcatc aaggtaaact gatgcctgcg gcggattgct
     5221 ctcgattcta agggttgcaa agttatctcc ggtcagcttc gcccttaccg gattcaccag
     5281 tcgctgcacc gcagccacca gctcctgttc atcggcattg gtccgctgcg actgcagaag
     5341 atcactgccc tggcagagac tgatacgtac ctggacacgg tttgctgagg cggtaatggc
     5401 agtgaggagc gtataacggt catcgcgcag gccagtgcac tcagcctctg gcgacgagac
     5461 acgcaattcg cgcctgatac gcaatggcag tatatcgggc ttcgggtagc gggaaagtaa
     5521 ctgttcgcgc tcaccctgtg gcagcgcatc gtgatctggc tgaagcgaat attcaatggt
     5581 gagtgggctt aagcgcccgc gcgtgacggc gctacgctgc agcgttaact ggtcggcaat
     5641 caggttttgc accacccctg acaggtaatt ctttgcttca ccgccggtta ccgcagggcg
     5701 caggatttcc atcacatact cttgcggcca ggatacccga ccgccggttc ccgcggagtt
     5761 cacttgtgca gcaagcgcaa cgggcaaccc gaagaacaaa agaagtgcgc gatacttcat
     5821 ggctgtgcag ccaggcgctt gatggtctgt atcatcgagg cgaaaccatt cgctcgcgac
     5881 atcgagaggt gcttggcaaa tcccagtttt tcgatgaatg acaggtttgc cgcagcaatt
     5941 tcggcgctac ccgcccccga aaacaatcgg accagcagcg cgatgagacc gcgtgtgatc
     6001 gccgagtcgc tgtcggcttc gaaaaaaacc cggtcgcctt cgcgtcgtgc gatgagccag
     6061 acctgcgact ggcagccgcg caccttgttc gcctcaacct tgtgggcgtc ttctaaagcc
     6121 ggcagctttt cgcccagttc aatcaggtac cggtaacggt cggcccaatc atcaaacatt
     6181 tcaaaatccg cgagtagttc tgcctcgcgg ctctctattt cagacatggt tcgtgttctt
     6241 aggcggtggg tccttttttt gccagggcct cgagtacccg ccgtacctta atattatgtg
     6301 gatcgagctt ctcggcaaat tctatgatca tgcgagacct gttgtacagc ccctgcaggt
     6361 aatagatatc cgcaaggttg atcaggtttt tcaccatgtc atggtcgcgc agcctcaggc
     6421 gttcgccaaa gtcagcagcc ttcttgtaat tctgcagcac cttgtggcaa tacgaggcaa
     6481 tgtagaggaa ctcagagtct gcaggcttga tgtgtatgta atcttcagcc agcgcagccg
     6541 ccttttcaaa ctgcttgtct ttcaggtacg ccttgatcag gtctcgcata acctctggtt
     6601 gcgtatcatc gatgcggagc gcctcttcaa gatcgccgat ggcaaccttg aggttagatt
     6661 ctttcaccgc gagacgggcg cggttcagca gctggataac atgctgtgac agctggccat
     6721 ggtcatgatg aacatcttct ttgtaaccga tacggagcaa cgaaaggtcg tcggtaagtt
     6781 cgccgtagtt ctctagtaca tcgcgtatgc gatcgagctg gccttcagac tcttcgacaa
     6841 tcttcaggaa ctgctgctcg ttttcattaa taatgcgcgc gccgtcttca tcgtagccaa
     6901 cgagcagatc atcgcgaccg tcggagccta taatgataac atcctttggc tccatctgca
     6961 gagtctgcac ttcgatatgg ccactaacgc cctgcgtacc gagtttgcgc agcgccatct
     7021 ctttttcgat aaaatcggcc ttacccttac gatagagcac tgaccagggg tgctctgcat
     7081 tgatatagta gagcagaccg gattcatcgt cgacaaggcc catcacgagt gaaatcagca
     7141 ttgaaccatc gaaaacctcg aacacgcgat gcagttcaac gaacgtgtac ttgagccagt
     7201 gttcaggaaa cacgtcctgc tctttgggtg aaagtttcgt gcgttcgatc accgactgga
     7261 agactgaccc aagaaccagg atacctccgg cgccctgcat cgacttaccc atcgcatcgg
     7321 cattaagaaa aaccgtgtgt ggcttatctt tcagggttat cgtgctcgtg atcacggtat
     7381 cgccaccgat atcgcggtgc catttgcgga attcaaactt cttcttctgt tcgacgatca
     7441 catcaacatt gaggttgggg ctgttcgcct tgtttgagcc cagcggattc gtcaaaagac
     7501 ttgtgagaaa gtaatcacca tcctgctgtt ttttgagcgc gttaacttct tcaaacgctt
     7561 tctgcaactc agcggttctc tcttttacct tgtcttcgag ggtctcagca tattcctgta
     7621 gcttctgctt ggagtctttg atcgagtgca ccatgccgtt gaatgaccgg gagagatagc
     7681 caatttcgtc ttccacatag ataggcaggg cgacatcgag attgccgtta ttgacctggt
     7741 tcacccccga tagcaggctg ttgagcggcg tcacgagcgc ccggctgaag aagaccggaa
     7801 agcccacaat gatgatcacg atcgcggcaa agatgagcag caggtacttg agccccagcg
     7861 ggtgaacata cttgcggtaa tcgaggtaat caaaaccgac ttcgaccagg cggtttttct
     7921 tataatcgat gcccacgtaa gctgtgtagt gtgcgccgga tttcttgtcg gtacggtagt
     7981 ggcgggcgcc aggtggtata atttcttcgg cgtaacgcag cagcttcttc ttgagctccg
     8041 caccgttgtc tgtagcgttc tctttcaggt aaacaagcat cgcgtcgctg aaggtggcaa
     8101 actggccacg tgtcttgccc aggaatttcg tcgccttttc ccgaaactgg aagtcgggaa
     8161 tcttctgcaa tttataacgc tggtgcacta ccggacgctg cagactaccg attttttgca
     8221 ggatggcctg cttgttattt gtgtcttttt tctctaatag tggtaacagg gcagccttgt
     8281 agccggcaaa tgtcggcggc gcagactgca ggattttctc aacgttatct ttgaaggcga
     8341 tatcttcggg caggtaaaaa atcttcatcc agatatttga aaactcgaag ccattaatct
     8401 gctcttccgt atcgatttct gcgccgctga ttgaagccat cggttcggtc ttcttcttta
     8461 ccaggtcata gccgatagcg tagcgcatgg cctcgggttc aagtccggtt tcacggtagc
     8521 ggttggcctg ctcacttttt actccgtcat aggctgtctc tttgtcctgg aacgaaaagt
     8581 agctaatcgc ctgcagaatg agcaggaaga ccacaacact cacaagaata attttggcca
     8641 tgaaggtcgt cttgtcagcc gtgttattca tgtagatgat gacgatgaca aacaaaccga
     8701 gaatcgtcga catatcaaac acggtctgga atgtcccgcg gtccatgcca ccttcacggc
     8761 tcagggtatt gaggtacgcc ggcagaatgg tagccgcaac gatacccacg atcaggccga
     8821 ccagcgccca gcggtcacgg ccggtgacag cgataacgcg ccatacaccg atgccaatca
     8881 gaatacccac ataaaccaaa atcgcgatac tgataacata gctggcatca tcggcatcga
     8941 aatcgtagta gtgtccctca aaatggtaga cgtatcccgc atcgtaagtc tgcaccataa
     9001 accatgcggc aaacgcgaga gcaatgacgg tcatcacccg cgcaagaatc tttgcaacgc
     9061 gcgtatgcac cggctcggga aattcaaaaa atatctgcgt cagcagcgtg actgcaagca
     9121 gaacaaagcc cacagtcgtc cagcgatgga atgccgcgag cggatggtag aatgtggaag
     9181 cgacaacata actggagaca aaaatgccgt agatgagcag ggcgcttgcg acgaccctgg
     9241 tggcacgcga cttgtccctt acctggaaaa atacagccgc acagataaat aggaagagga
     9301 ccggtatgac tgacccgacg gtaaaatagt caaggtagac actctggaaa aaattcatgg
     9361 gcaagcatga taaactccgc cgcgtctgtc aagcggatag ccttaaatcg gctgtttttc
     9421 aacaggctgc tagtactgca ggggcggcag gtcgcgcagt atgtcagcgc gggtttttac
     9481 cggtggcttc tcagcccgtt tagcccgggt cgccgattca cttcccgcct tgggcagtat
     9541 ctcttcgacg ctcatcatgc gggtgtatga gcgcagcgga ttttttgccc cggtcggcat
     9601 ttcatcgact tcggcagctg ggccgctata agtagtcgat acgccaccct gcagggcctc
     9661 gagcgagatg cgcggctgcg gcatcttgtt ctcggtactt tcaaaaacat gtttggtcag
     9721 gaaatccggc gggttctcaa tcttgagctc catcagcttg accttttcgc cgatcaggtt
     9781 attgatctgc tgctctgcag caatcttcag cgtgttccgg taagaccgtg tgctctcgat
     9841 gccatcgaca agaccgtgga gctccgcgat ctctttgtcg atctgctcaa cacgtttgat
     9901 cagatgtatc acctttaccg gctgcatagc gtacgccggt agcgcgtctt gcccggtcaa
     9961 gtgaaatggg atttcatggc aaacggttat gtcaccaggt catggttcga caagctcacc
    10021 atgactccgg gtcaccctga gcatgtcgaa gggtgaccgc gacctatgcg ctcggtacct
    10081 tctcagcgtg caggttcaaa tctcattgac cccctgtctc tgacgacaaa gaaaacctgt
    10141 gctatttact gaattgaagc tcgccgagcc catacaaaag cggctcgccg agaagggcta
    10201 cccggagccc acccccatac aggccgaaac cataccgctg gccctcaaca accgggacct
    10261 tatcgcctgc gctaaaaccg gttctggcaa aaccctcgct tacctgctgc ccattctcca
    10321 gtacgttcac gagagcggcc tgaaacaagc ggacgccgag caggacggtg taaatccccc
    10381 ggtggcattg gtgcttgctc cgacgcgcga actggtcgta cagatcgcag aagaagccga
    10441 attcttcgca gcagccgcag gcctgaaact cgcgaccatt tacggtggcg tggaccacga
    10501 caagcagcgc cgtgaactca gctcgggcgc gcacctgatc gtcgcaacac ctggtcgcct
    10561 gctcgacttc attcgcggtg gcgaggcgaa cctgtcgaag gtcgtgcgca tcgtgctcga
    10621 tgaagccgac cgcatgctcg acatgggctt catcgacgac gttcgcaaga ttctctcaaa
    10681 gtgctcaccc aacgaaaacg aagaccgcca gttttccctg ttctcagcca ccattaactt
    10741 cagtgccctg tattcggtgt gggagttcat gcgtgaacca gaagaaatcc tgatcaaccc
    10801 tgaactcatc gatcacgcaa atatcgcgca agagatgctg cacctcggtc aggacgaaaa
    10861 actgccgtac ctgatacagc tgctcgagaa caacaagctc gaaccggtca tcatcttcac
    10921 caacagccgg cagtacgtcg atgtcctggt gaaaaacctc aaccaccaca acatcgcggc
    10981 cgcgggcctt tcgagcatgg tgacgcagaa caagcgcctc aagattctcg acgacttcaa
    11041 gaacggtaag ttccgtgttc tcgtcgcgac cgacgtcgcg agccgcgggc tgcacattga
    11101 agacatacag cttgtcgtga actacgacat cccgatggac cccgaaacct atgtgcaccg
    11161 tatcggccgc acggcgcgcg cgggtaaaac cggcaaggcg atcagcgtca gctctgagtt
    11221 cgactacgac tcgctggcaa aactcgaacg ctacctgaaa tacaagattc ccgtggcgca
    11281 acccgaagcg cgttacctcg aaaatgtcag ctttgtgcgc atcgtgcacg tcgagcgcca
    11341 ggacggtgcc gaccacgtac gcggcggcga tcgtcgcggt gcacgcggcg ccgagcgtgg
    11401 cgggcgtgac cgcaaacgcc acgaaggtgg ccgtgggcca cgtcgcgaag agcgtagcgg
    11461 cggagaagca cggtctggcg aaggcagaaa accgcacgcc aagggcgcac agggcgcacc
    11521 gcaacaccag aaacaaaaag acggcgccta ttttgatgcg cgccgtggtc gtggcggcaa
    11581 cgagcagcgt ggccgcgatg gccagcgcgg ccagcaggct ggccagcgcc gcgggcgcaa
    11641 cgacaacttt gcaattgtac cccgtactcc ggaaaaagtt ggctttttta cccgtatcgg
    11701 ccgcagcctc aaggcgctgt ttacccgtaa gccggcaaag ccacagattt ccgaacgcac
    11761 acggcgcttg ctcgaacagg aaacgcatgg tggcggcgat aagccgcgcg gcaacaacaa
    11821 gcgccgccgg cgcggtggta ataaccgcag gggcggcggg cgcgaacgcc agggcaaccg
    11881 cgagcgacgc taaacatctt gcgcgcgctg aggaaaagcc tatacccgct ttccctcgtc
    11941 ggcattgatc tgctggcgta tacagctgcg tttgtctgtg cggtcaatct gcgtatctgg
    12001 ctcgggaaaa cctttcctgg tatcccggat ttttcgggcg atgcgatgcg catgaatgac
    12061 ctctacttca tgcctgcgct gctgctggta attttctttt acgaaggcct ttaccgccgg
    12121 cgcatgccat ttatcgacga actgcgggag atcaccaagt cactggcgat tctcacggtt
    12181 ttcctgtttg ccctgatttc tattggcaac ctcgcgcacc tcgtcagccg gctcaccatt
    12241 cttttgatgg cgccgctggc tctggtgact atcggtatcg cgcgctactg gggtaagttg
    12301 ctgctgcacc gcttcggcat tggcacacag aaactgctga tcatcggtga agtggcggca
    12361 gcagcacaaa tcaaggcgga gctacttgcg gaaaagacgc tcggctaccg tttcgccggc
    12421 tttatcccca tcgatcggcg cgaagaaaac ctctggcata aacagcaggc cgccggtctt
    12481 acccgcgcgg gcagcgccga cgaggttgag gctattctcg cccggagaga ggtggaatgc
    12541 gtgctgctgg cgacgccttc gtcgagccgc gaaaaaacca gcgcgctggc agacaaggtc
    12601 taccgccatg tacaccacgt gctcatgata cccgaaatgg gttcgggggc actgctcaac
    12661 tcggagctct accacctgtt cgtcaaccag atgttcctga tcaaggtgcg caacagcctg
    12721 acagaaaggc cggcgcgctt cagcaagatg ctgttcgaca tggtactggt cgttctatcg
    12781 ctgccggtca ttgccccggt gctgctcgtc attggtatta tggtcagggc gacttcgccg
    12841 ggccccgcgc tctttcgcca ggtacgtatc ggcaagaacg gcaaagagat ccgggtcttc
    12901 aagttccgca gtatgcaccg cgatgccgag gcaaaattgg cagaactact ggcgaaagac
    12961 cccgcagcgc gcgccgaatg gaaaaaaacg cataagctcc gccatgaccc gcgggttacc
    13021 aaactgggtg catttctcag aaggacttca ctggacgagc tgccacagct gatcaatgtg
    13081 ctgtgcggcg acatgagcct cgtcgggccg cgcccggtta ccgaaaccga gctcaagacg
    13141 aaatacaaag gtaaggcaga ctactacaag ctggttcgcc caggcatcac cggtctgtgg
    13201 cagattagcg gccgcagcga catcagctac gaacagcgcg tcaacatgga cagctggtac
    13261 gtgttcaact ggtcgctgca cattgacctg gtgatactct acaagaccat ctgggtcgtg
    13321 ctgggtcgcc ggggtgctta ctaatatgag aaaaagaccc ctccccgtcc tacggacacc
    13381 ccacccctcg tccaggggag gggccagggg aggggttaag ttctctttgg tcatacccgt
    13441 ccgcaacggc ggcgctctct atcggcagtg cctcgaatcc attgccgcgc agagccttaa
    13501 acccgcagag gtgctggttt ttgatacaga atcagacgat ggctcagcta cgctggccgc
    13561 cgcggtactg aaaaaaatcc cgctgcgtac cttcagtgtt gcgcgctcac agttcgacca
    13621 cggcggcaca cgcaataggg cgctgcaggc cgcccggcat gaatgggtgt tattcatgac
    13681 gcaggatgcc atctgcgccg atacagaagc atttgcgcac ctgctggcat ctgccagcgc
    13741 gcagaatgtt gtggctgttt acggccggca attaccgcac agcgatgctt cgccgctcgc
    13801 cgcgaccgcg cggggtttca actatggcac tgaacgcgtt gagcaaaaca tggcgctggc
    13861 acagaaactc ggtatcaaaa cctggttctg ctccaatagc ttctgcctct ggaacaaacc
    13921 tgcgctcgaa aatgccggcg gattcgccga gaagctgatt ctgggcgaag acatgcacgc
    13981 tgcggcaagg ctggtacagg caggcggtaa ggtaatttac gagcccactg cacaggtgag
    14041 gcattcgcac aattactccg cggcagaaga attttgccgc tattttgata cgggtgtttt
    14101 tcacagcctg caccgcgaac tactcttccg cgcgggcaac ccctctaaag agggattacg
    14161 ttttgttctg ggccagctca ggcagctggt gaagttccgt gcctggctgt cggtattgag
    14221 actgcccttt catgttgcgg caaaattttt gggttacaag ttagggcaga actacacgct
    14281 gctcggcagg cgcctgtcaa gggcgctcag tatgcacaag aatttctgga actgaaagga
    14341 acacacatgt cgctatctga gagtacccta gccggaagat tcgccttcgc cggcctttgc
    14401 ctgatgggca ttggactcgc cttttcgatt tccattgcgc agactggcct cggtatcacc
    14461 tttgttattt tcatcgccac gcttttttct gccggccgaa agagcttaag cctaaaaggc
    14521 gcacactggc tcaaactact ctacctgttt tttggcggct gggtactctg gcggattgtc
    14581 cacgtgctga ttgcggcgcg gcctggcgca gaacttttcg aagcgcggga agtctggctg
    14641 atgctgattc cggtttttat ctggttctat gcctcgtcgc gcaacaggct gcatacggtg
    14701 ctcgcggcat ttgtgctcgg tgcggcagtg tcgtccgcct ggggcatctg gaacatgcgc
    14761 aatgagatct tcgacatgtg gacgcgtggc cggggcttaa gcactatgca ccaccttaac
    14821 tatgcgggcg tcgcagcctt tgccgcgctg ctgggactgg ggctcagctg gtcgtactac
    14881 tacagcggta aaaagtttcg cgcagcgctc atccttttcc ttgcactgct ctcttttgcc
    14941 gggttatggc tgacaaaatc aaagggtgcg attgcagcct ttgcggccgt agtgcccctc
    15001 tttatctacc tgcagctgca taaccgggtg caccgctggc tcttcatcgc gctggcagtc
    15061 gccggcgcgg cgtacctggt gccgcggctg cccgagtcga ttgccgaaca gtaccgcttt
    15121 cccccgcccg aagtgcacgc agggtcgcag gcagaacgcc gcgacctgtg gcagaccggc
    15181 ctcgcgatga tccacgacaa cccgtgggta ggttacggcg aacgcggtta caacgaggct
    15241 tacccccgct accagctacc gggggcaacc ggcgtcgcgc agtgggatga aaaatcgcgc
    15301 gaagcgtcac acatgcacaa cgactttatc aacaccgcgg tgctctatgg ggccattggg
    15361 cttttactgc agctgggcta ctacttcctc ggcttctttg cttacctgag ggagagattc
    15421 cacattcggc ggcagagcga ccgcccgctg gccgcagccg gggcgagctc tttggtcctc
    15481 atggcactga tgggcgtaac ccagtgccat ttcacttcag agattgtcca gatgtcgttc
    15541 tgggtggcac tcggcagcct gtttgtactg ctcgacagcg accgatcggg taggggcgaa
    15601 gaaacctgat tttttgggtc cggtttttga ttgactacac gtcattgaag gccgttctcc
    15661 catacgctgt ttactaaata gcgaatataa aatgtttaat attgaggaaa atgtcgttta
    15721 tccgatgcat ggcgtcggca agatagtaga aatcgctacc aagacgattc tcaacaagaa
    15781 gaaaaagtat tacgtcgttg agctcattaa taccaaaatg cgggtcatgg tacccgtaga
    15841 caatgccgag accattggcc tcaggcatat tatggataag aagcagatcc ccaagatcgc
    15901 taaactactg caggaagagt acgtcgaaca agaagaagac tggaaaatcc gttaccagaa
    15961 caacctgacc aaggtgaagt ctggctctat cacggctgtg gccgaagttt gccgcaacct
    16021 ctacaagcga gcccgcgata aagagctctc ggtgatggag cgtaagctgt acgagtctgc
    16081 ttattccctg atgatcaacg aagtggcgct ggccaaaggt atttccgccg aagatgccag
    16141 caatatgatt tccgatatct tgtcgaaata gctcctcggt gaacctaaga ctggtgcatt
    16201 aagatgccac cagttttagc gactcaatag gaggatcaat gaaaaactcc cagaataatt
    16261 ccggagccac cacaaatgcg ctcccttggg tgcttggcgc cctggcaggc atggtagccg
    16321 ccggagccct gcacccgtac ctgaaagatg cagcagcagc ggtagcttac ggtatcgcag
    16381 ctctgctgac ggttgccagc tcagccgcct tcggcttgct gtggccgcag ccgcccaaac
    16441 aacctgcagt gcagagcgta ccagtgaccg gaggcaacgg tcgcgctgaa aaagcgcagg
    16501 ctgcagacga caaaccagca gccgtagttg aatcgaagac gaagattctc gacacctcga
    16561 tcatcatcga cgggcgtatt atcgacatcg cagcgacggg ctttcttgag ccgcctttcc
    16621 tggtgccgaa cttcgtactg cgtgagatcc agctgatctc cgattcgccc gacgctatca
    16681 aacgcaaccg cggccgtcgc ggtctggaca tgctgaacca gctacagaag cgcagcgacc
    16741 ttgaagtcaa gatctcgtac aaggactata ccgaaacacg cgaagtcgac cagaagctta
    16801 ttcgcctggc gaaagaaacc ggcggttacc tggtcaccaa cgacttcaac ctgaacaagg
    16861 ttgcagaact gcagggtgta cgcgtgctca acatgaacaa cctcgcgaac gcgctcaagc
    16921 ctgtcgtact gccgggtgaa gaactcgaac tgcaggtcgt gaaagaaggc aaagatgaga
    16981 accagggtat tgcgtacctc gaagacggca caatggtcgt ggtcgaaggc ggcggcaagc
    17041 tgatcggcaa aaaagtcaaa atcaacgtta cctcagtcat ccagaccaat gccggcaaga
    17101 tggtctttac caaactggca caaaaatgat gcgccgtagg ggtaggttta aacctacccc
    17161 tacggcgtgg taacatgaac gcattgcttt tactcatggg agggtcgggc cagcgtttcg
    17221 gcgcggccac acccaaacan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    17341 attacgagaa ccgccaggag ctgcggcgtg tgcagacacc gcaactgctg cactacccca
    17401 cttttatcgc cgcgcagtcg gcggcgctgg gccgtggcca gatggagctc gacttcacag
    17461 acgaaggttc ggtttgcctg tcgctcggcc tgcgagtcgg cagcttcgag ggtgataccg
    17521 aaaacctgaa aattaccttc cccgaagacc ttaaaccaga taaactttaa ttctccgcct
    17581 gtctctgcgt ggcaggtttg cccatgaaat cgattctgtc aggtctggca gccctaccga
    17641 tagtcttgct gctggcaaat gccggtacgc aggcgcagac aggagtctct gtgactccgg
    17701 acaacgaagc gcagatacta aacctcggcg tcatcaatac accgtacagc gaatacacgc
    17761 catttatcac ggcggatgaa cgttaccttt tcttccagtc gaaccgtccc gacggcgtca
    17821 ataactcagg ggattttgat ctctggtaca cagaaaacca ggtcaccgaa ggagtcccca
    17881 aatttaaaac gcctgccaac gtgggcctgc cggtgaactc agcttacttt gacgggcatc
    17941 cgacattgcg ccgtctacct tcgggggagt acgaaatgta ctttgcctct tttgccgaag
    18001 aaggccgacc tggcccccaa ctgacaaacc tctattacac tatcttcaaa gacggcaaat
    18061 ggacacagcc aatgcccgtc gttgaggtca atactgactt tcacgaccgt atgccgtcta
    18121 tctctcagga tggccgttac ctcttcttca gctctgaccg tcccggtggg tttggccgtg
    18181 acgatatctg gctctctgaa tacgattttg cagcaaaacg ctggggcaag ccgcgcaatg
    18241 cagggcgcac catcaatact cctgcctcgg aagttactcc cgccatccac tctgacaata
    18301 tcacactgta tttttcttca gaccgtgcag gtggtgtcgg cggttacgac atctacgtca
    18361 cacagatgct gcagaacctg ccttcggcga gcatgacaaa cgagggcgaa tcgatgtgga
    18421 agcgcccggt gaacctgggc aaaccgtata actcgacctt tgacgacgag taccccacgg
    18481 ttattcgtaa cggtaactac atgtactttg cgtccaaccg cgaaaatggg caggggagct
    18541 tcgacgttta ccgtggcaag gtacctgagt ttgccaaacc cgaagtggtc gtgacgctca
    18601 aaggtcgcgt caaagagaaa ttcagcgaca agggaattga agcaaatatt cgcataaccg
    18661 acctcgacgg cgagaagaat ttttccacca accagcccga aggtctctat agtgccgacc
    18721 tgatcaacaa gaaacagtac aagctgacgg tgacggcacc gggctaccag ccgatcgaat
    18781 acatcgccga cttgcgcgaa atccataccc cgattaccat acagaaagac ttcgaaatgg
    18841 agcgcgtgct cgcgttgcca aaagatgtgg tgctgcgcgt aacgtttgaa aacgagagcg
    18901 gcaacagcct gaaacccaac gcgacctacc gcctgccgcc gggcgtggcg gacgatacga
    18961 ttcttccgta caagggtagc gtagcgcaac tcaaggttcc ggcaatgtca cgttacagca
    19021 aaccagaaga ggcgcttgcg gcgctcgacg caatgcggct ctcggtcaag gcatcgctca
    19081 agggctacga agacctcgct gaagagcgag cgatggcgca actgctgcgc ggcagcaatg
    19141 gcgaactgaa gggcatcatt gacgtgaagt ttgtcatgaa gggcaagcaa gaaggtgtcg
    19201 aggtcatacc taccaaagga cctggcgggc ttgaagcaat cgtctacttc tcaaccaacg
    19261 tctcaaaccg tctcaataat gagaaggctt cgggtctcaa gaacgtcgtc gacctctgga
    19321 acaaacagcc gcacaagtac gtttacgttt acgggcatgc ggacagccgc gggacatcgg
    19381 ctcacaacat gaaactctca aaagcgcggg ccgagtttgt aaaacgccgc ctggtagaat
    19441 acggtattcc gagcgagatg attatcacgc gagccttcgg tgaaacccgt ccggtcagta
    19501 aagacgaaag cacgctcgag gggcggcgca agaaccgccg cgccgagatc cacttcgatg
    19561 caaccaagcg caaggacgaa ggcgaaccca ccg
//
LOCUS       DAOQJF010000010        19345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1291_length_19345_cov_5.129082, whole genome shotgun sequence.
ACCESSION   DAOQJF010000010 DAOQJF010000000
VERSION     DAOQJF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 19345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19345
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1291_length_19345_cov_5.129082"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLF85_01125"
     CDS             complement(<1..1120)
                     /locus_tag="PLF85_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18283.1"
                     /translation="MQRGIFKVSLCLFSLLFFQAGAVPWREKNALRIASTLPKRLIDS
                     IMAQGNWNILFQEGPFSEFQRQMAIGGHFALRGEVSQAEFYYRRAETALDIAYASLRS
                     RFEWPRSPLQLKADELPWGENRDTFRDYLLCRLQLYIESGLAGHDLGKVGLERLAETV
                     ARTESQMSQSLKDKDADLDNFVRLLRGSNTLRATSGISQGLRATRFLELSAAIPPSAR
                     NYWRRRTVFFQIQEHLYYGNLGIARALTDFLFSRHGDQSDPLAIARFWIQSSDFVAAA
                     DLLEKEFASSEVRSGENYPDFLRISETLQNLLVWQRQYLRAAEIAQVSRDLLNGFIAG
                     NQVPREEFVSVRRSAANQKLRSEMLHFLATGKCPSTLQL"
     gene            complement(1130..2794)
                     /locus_tag="PLF85_01130"
     CDS             complement(1130..2794)
                     /locus_tag="PLF85_01130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE18284.1"
                     /translation="MALTNVTAIVSNVKNFYGKFEKYLADKAFENNNISTDKMDQDQV
                     ANYDLAWVAAEIFAIDEMVAYPAKVGKGPGSIEENLSLFFIADALQDIIGRLRAAAQK
                     MGVSQAQFDSDLKTSEVYGFIAEYSSEAFAKKISDALASNKDYGAYGLNDDQAMMADT
                     FRKFAEGQVKPLAEKVHRQDLTIPDEIINGLAEMGCFGLSIADTYGGFQSEENPDHTS
                     MAVVTEELSRGSVGVAGSLITRPEIVAKAISAGGTEAQKQKWLPLLASGEKKSAVAVT
                     EPDYGSDVAGMKVAATKVDGGWRINGVKTWCTFAGMADVMLVLARTNPDMSLKHKGLS
                     ILLAEKPPTRDHEFDYKQPEGGRVHGKAIATIGYRGMHSYEVVFEDYFVPDENLIGGE
                     AGLGKGFYLQMAGFAGGRLQTAARATGVMQAAVEKALQYALDRKVFGKPILSYGINQH
                     KIAKMAAITQAVRQMTYKSARLMDKHEGSMEASLVKFYSCKISEWVTREAMQIHGGMG
                     YAEEYEVSRLFVDARVFSIFEGVEEVLALRVIARSLLARALGLKAA"
     gene            complement(2795..4054)
                     /locus_tag="PLF85_01135"
     CDS             complement(2795..4054)
                     /locus_tag="PLF85_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNE18285.1"
                     /translation="MAKNKRVYILGGYQTDFARNWAKEGKTIQALMHEAMDGAFSATK
                     IDPADVGSIHVGNFAGELYTMQGHLGAMTVNYSEKLRGAPTSRHEAACASGAISTLVA
                     RTEIEAGHYDLSMVVGIEMMKSVDSKTGGDFLGTAAWYDKEAKGVQFPFPKLFGRLGT
                     VYEELYGLKYDHLAEISAINYENARKNPLAQTREWYMNKQHALSRGNFNMEVGGIIHI
                     TDCSQVTDGAAVTFLASEKFAKRYAKEHGLKLKNIPYIMGWGHATAPMLFDIKTEEAY
                     AAKKEGGYILPHTRKAIVDAYKRAGIKGPKDLDLVETHDCFTTSEYAAIEHFGLTAPG
                     EAYKAIENGDIRIGGKLPFNPSGGLIGAGHPVGATGSRQLLDLYKQLTGTAGENQVEG
                     AKVAAALNIGGSATTNVVHIVGVEKGK"
     gene            complement(4068..5369)
                     /locus_tag="PLF85_01140"
     CDS             complement(4068..5369)
                     /locus_tag="PLF85_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNE18286.1"
                     /translation="MAKSTRVIKKVGVLGASGNMGSLSGGIFAQADIECVFFARSLDK
                     ATAGLEAAVGQARSDVLRKYIKAASYDDLEKEIPSCDWIFEGLAEDMAIKNEFFAKIE
                     KLKKPDAIVSTVSSGLSIRKMGEGRNENFRKNFMGTHFYNPPGKLPANELIFHPDVPA
                     DTREFVYDFCEKVLRRVNIITEDTAAFAGNRIGFQLLNDAAIHAEKYGVDKIDYLLGP
                     YTGRALAPLATIDLVGLDVHKAIVDNVVANVKDERIDTYKMPAYMQKMIDAGMLGRKA
                     KGKGGYFNRDAEKNKTTLNIADLSFAPTKKDKIDWVEKTKQAIQDGIYPRAIDLIKNA
                     PGEEGKIVRYFILGYVAYSFARIGEVTPEADGIHGIDRVMSSGFSWLPPSGWVDLFGG
                     AKETAALIEKAGIPVPAQLKAIKEKGKICRVADVSKFLIGR"
     gene            5544..6029
                     /locus_tag="PLF85_01145"
     CDS             5544..6029
                     /locus_tag="PLF85_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18287.1"
                     /translation="MRPFLLMILLAGGMLSAQAENSGTTPPRVEYEKQVPPLPLRKLV
                     DAAVLMLEARAYEQFIRTFVPESDRGRFEKAYTREGGVDYALWGQEKGSRLLTVLRSL
                     ASADAVVSSTRVCYFSPGEAKPLLSFIQVRSMWLIENSSRCTDRRPAKPPVAPAEAPA
                     P"
     gene            complement(6053..6409)
                     /locus_tag="PLF85_01150"
     CDS             complement(6053..6409)
                     /locus_tag="PLF85_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18288.1"
                     /translation="MQTITSYLVGAFALLSIPSCASSTWREGFSGSDRLYLVGEGHAR
                     EDLPQVQRSAMAREAALIDAMSHWPRHCGALSNDDGIASFRVENQKQRLIECEGTTCR
                     ARIVIERSGLREKCKS"
     gene            complement(6415..7254)
                     /gene="kdsA"
                     /locus_tag="PLF85_01155"
     CDS             complement(6415..7254)
                     /gene="kdsA"
                     /locus_tag="PLF85_01155"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNE18289.1"
                     /translation="MMVAQNPERDFISGHKIGGSNKPFFIAGPCVIESEDLLRTVAKE
                     VRRVADEQKILIFFKSSFDKANRSSIDSFRGPGMREGLSLLKKVADDFKLPCLTDIHN
                     PEEAEVAAEYVDMLQIPAFLCRQTDLVVAAAKTGKWTNIKKGQFVAPEDALQIADKYR
                     KSGSDKVTLCERGYTFGYNNLVVDMRSFEIMRAAGLHVVFDATHSTQLPGGGKVSGGD
                     RRMAFPLARAAAATGLDGIFAEIHPNPPEARSDATNQLYLSDFARFVDNILKIDRLVK
                     SDV"
     gene            complement(7257..7577)
                     /locus_tag="PLF85_01160"
     CDS             complement(7257..7577)
                     /locus_tag="PLF85_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18290.1"
                     /translation="MPICSLLAAIFLLACASGRYQVPDAEFFTDGYALDKQTLQVVES
                     HEDASLLLPAAERYARNCESAEVKARNRHRVAYPQSRDEGKKLSTRFDKNAGCTVRMA
                     FRKE"
     gene            complement(7631..8338)
                     /locus_tag="PLF85_01165"
     CDS             complement(7631..8338)
                     /locus_tag="PLF85_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE18291.1"
                     /translation="MLTVENLSKHYNSRRAVDRVSFTLRRGEISALLGMNGAGKTTTL
                     RMLTGYITPTTGEVLLNGENIIAEREKLLSHIGYLPETPALYNDMTVEDFLVYMYRLR
                     VAEQQGETDAIEKALKRCALGERRHDYIASLSAGFRKRVGLAQAIIHNPEVLLLDEPI
                     ADLDPRQIREIKSLLSELRQDHAVLVSSHILKEVHEFADRIFLMHEGRILAEAESKAI
                     PAGELENWFMRHTEAAA"
     gene            complement(8342..10009)
                     /locus_tag="PLF85_01170"
     CDS             complement(8342..10009)
                     /locus_tag="PLF85_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18292.1"
                     /translation="MQIRSLLLLVTLVSGVAAARPADRVPADTSIYIEGAPLTESLTA
                     LRTLVANIAGEGIWSMMSANFEQKMGVNMLDPQKLEEIGIKTSEQWAIAINMEIEAAG
                     NPSKPEFVMIFPIQSNTKFYDFLKAKITESQMPINTEVEAGRVLNFGSENDPGYLVRT
                     DDALLVSNKKEMVMAMRSKVSSPITDASYYTSMRSHLLSRNGKKQPMMSFYLNPKLIV
                     SSLKAQSDLLRNLQKELNKGDETAPAIDDNSPYVAEIRDNLQSSGGALVANAERISFY
                     FSYKYKEGYLSDTSKIYPRIIQVKTEPLASDTAARNPVNYTLIKLNVMGMIDLFKSLS
                     PVFGEKYATAIQEANTNLGMDFESQILNSLRGNYNFQLLNIPTEEKVREVGAWELYGA
                     FGIKPGTAKNWVRLLKASEKMAKKAEANKKQKTKFKFEEAEDGEFVSISGPGNMVNGK
                     RQTVTVVFLIRENEVIVSNSKANALKATKGGSATLSERLAKIPYESAQGVFFLDLQQV
                     FKAVMKSKQGSSLKAYGPMLEKMKNFSIISTVQGDFATAETMLQMRK"
     gene            10086..10853
                     /locus_tag="PLF85_01175"
     CDS             10086..10853
                     /locus_tag="PLF85_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18293.1"
                     /translation="MAFEIKGFRGRRRATMQAGQTEIAPATAESEQLPLGIRLQHWVN
                     ENPRAIRNSIFVLAGITVAVVLVLVLRAAAKDEHSRRLHAALEKYEQIKILPKGDARA
                     LQMKEFGQSLKPVCEQTIATLESAAACFTAGSALLEGRDPVAAAALFARAASGYDTDA
                     MASVAGFMQAQALESARDFEKALLVYKDLEKKYALIKKTEMALFHQGRMLYYLGRYDE
                     AEEKFAKLSRDSEGKEFAASSRNYISLLNAERAQLKK"
     gene            10855..11484
                     /gene="hisG"
                     /locus_tag="PLF85_01180"
     CDS             10855..11484
                     /gene="hisG"
                     /locus_tag="PLF85_01180"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNE18294.1"
                     /translation="MAEEKLKIALPKGRMSDESLEFFSSRGLCSLSRTQDGRELILHD
                     EKSNIDFYLIRSKDVGAYVEQGAADLGIMGLDLLLEHNFDVFIPAALPFGQCRLSVAY
                     PKDRADWQNKPELRVATKYPRLATEYFFRRGFNIRTIELYGSIEIAPLTGLSDVIVDL
                     VSTGATLKANNLVEEEVILESTARLIMNPSALALGRKRLMQFIDTLFQV"
     gene            11497..11700
                     /gene="rpmF"
                     /locus_tag="PLF85_01185"
     CDS             11497..11700
                     /gene="rpmF"
                     /locus_tag="PLF85_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNE18295.1"
                     /translation="MAVPKRRLSHSRRRKRRAHHAIGKPNLRPCANCGAYGIPHRICT
                     ACGYYNGRQVLIPRVKKAKADKE"
     gene            11704..12267
                     /locus_tag="PLF85_01190"
     CDS             11704..12267
                     /locus_tag="PLF85_01190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE18296.1"
                     /translation="MLRPHGKASLVGQHLHHDNCFACGALNNYSFHIPSTFDEQKGEV
                     CFTYQLRKEHEGAPGYAHGGGLALILDEAQGVLCHHLGHFVMTDGFSIKYHKATPLYK
                     DIKVTAWLTSARSKRLYTKARIELGDELLVESKIKWYIIPERLLERRFGEDGMGGRLK
                     ERLEPNRVRAKEIRRRLRAEKAGTAAK"
     gene            12335..13246
                     /locus_tag="PLF85_01195"
     CDS             12335..13246
                     /locus_tag="PLF85_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNE18297.1"
                     /translation="MRMLVVGSVAFDDIKTPFGEKKNVLGGAASHFSIAAANLIEPKI
                     IAVIGSDFPEKYLTYLSDFGIDTAGIERSSDKTFHWSGFYEHDMAVAHTRSTELGAFA
                     SFEPKLTEEDKLRQVLFLANIDPVIQYKVLKQMKSVQLVGMDSMNFWIESKKKDLWKV
                     IKAVDILFLNDAEVRMLAGEASIIKAAKAIQKQGPGIVIVKKGEHGVMAISQNHIFVS
                     PAYPTEKVIDPTGAGDSFAGGVMSYLVANSQPGKKISDELLRRAVAWGSATASFAVEG
                     FSTTGIAKADKAGIEKRVEALRKLSSF"
     gene            complement(13319..15202)
                     /locus_tag="PLF85_01200"
     CDS             complement(13319..15202)
                     /locus_tag="PLF85_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE18298.1"
                     /translation="MQKSHMKRSMGPRTLLDQSYEPGPGFLYIPPIPRSRFDFLSQVQ
                     SAFVNKVVSFTFPDFSHCFDYSVFEIIDGHYMRVTASSSRFQLRELAEHKGVEVFKKF
                     ISRTRPLAIGHEKNLSAQMMRQSLYNIAHDLPPMVEFLHIASEERLHGAMKRLAPKGV
                     RYAVGIPIIIDRKPVGVLWGIHRRSFSADQRRDVRQQMISLSRSIDFIISEELVEGAD
                     AYSARRKIEKHDPTANLETLVYTHVPDQEKPVKSMVLYSNRYSEKYRQDTNYIVPTVK
                     GYNISLKRYLPEHLNGQDRILLMLPGFFCNRSIMDRLAKEMALEHGYPVFTLDVRGRS
                     KETLPNKIFNNYHWSVDNYIWEDFPAALQWLKATHPGKKIVVFGHSMGGLIPLFYSAA
                     YDKHGKKRSGELAIKPESMIDGIVSITSPIYIRIAAENAWFNMFRSSAKMLTGNALAD
                     PIMRMVNFTLTSTLGGIDLNKFFTFLNSISASIRTVSFDVTYRLPTIKEFIGYEQITP
                     PEWYFMMEDVFCEESMLVISQFIRSLIDGDNFMSIDKEINYTLECNDLEIPHFTVVGT
                     VDEIAPPDTVRQGHLAGKSPKNRIAEYKQGHLGIITHPETVKQIAIDTDEWIRALPAN
                     YIS"
     gene            15322..15996
                     /gene="infC"
                     /locus_tag="PLF85_01205"
     CDS             15322..15996
                     /gene="infC"
                     /locus_tag="PLF85_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNE18299.1"
                     /translation="MQQRPNFSPPRSPDGGGGGQGRFNRFAKLRRPTREHRINEDITE
                     SQVRLVGEKGTELISTRDALARAKAQGVDLVEITKGQEVPIVRLVDYGKFKFEKAKKE
                     KEAKKKQKVIQIKEIKMGPKIDTGDFDRKCKEAISFLSEGDNVKVTMKFRGREMQHTA
                     LGVEKLTDFYNKVAEYAHMDKKPNLEGRIMSMTMRSKGAKPKAAAAPSEKPAAPAPEA
                     APKPQA"
     gene            complement(15997..16815)
                     /locus_tag="PLF85_01210"
     CDS             complement(15997..16815)
                     /locus_tag="PLF85_01210"
                     /inference="COORDINATES: protein motif:HMM:NF016212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNE18300.1"
                     /translation="MNKPVMEFFTALQNIRTESDLPRKLAALSELNEALASDAVQADH
                     AAATEPIGAPSYAAICRIVHGSQVERRRNLGTAEGRAVFLHALAHIEYSAIDLALDSC
                     YRFRHLPPQYYEDWLKVTFDETRHFVMLQKLLGQLGYHYGCFSVHTGIHDAMARSAHS
                     LRQRMAATHRHLEANGLDAHPELARKMQLFDDPLAKQISAALKVIFDDEVTHVHAGDF
                     WFRYACTLDEVGTGIFAEDVQHAIPGTRFPRPNLNREARLRAGFTSAELDALSG"
     gene            16857..18143
                     /locus_tag="PLF85_01215"
     CDS             16857..18143
                     /locus_tag="PLF85_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNE18301.1"
                     /translation="MARGVRLCITGSEVMNGFVLDRNTQFFASELYALGLELVESRIL
                     HDDPQHIMATWKEFAATGDIIVNSGGLGPTTDDLTVDLLCEWQKDTPVYEPHAEKRTR
                     YFFEKRAKLQPNSYSLETALRQARIPSRAVAIKNPVGLAPGIYLPELNFFALPGFPDE
                     IKGMWPEVLRRIEEQKTVRSVTRIIPVWGVGESQLFTELRSLAPDIDKKVSVGVHALP
                     WGCRLFLRAKAGDAGSEAAVAQFAGTLESHFAGQVVDDPLGGVIAYCKEKQLKLAVAE
                     SCTGGLAAKQITDIAGVSAIFTGGVVSYANEAKTALVDVPQDLIVAHGAVSAEVAAAM
                     ARGVSQKLETDLSLSFTGIAGPDGGSESKPVGTVFVGLHDARTGKTLVARFLFPFGRE
                     RFRNATVATGFIALWQYLHQPNGGGVFPLSTFHSAL"
     gene            18277..19116
                     /locus_tag="PLF85_01220"
     CDS             18277..19116
                     /locus_tag="PLF85_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18302.1"
                     /translation="MRQKSVHTIVFFSMIGFLLLGGASPVRQAPLWAQDSESFFDDEL
                     PEEKPKSGDLKSKPIEIREDDEAERKKALKKTNEKGEVVLPKVEKPKDDGPIEQVAPL
                     SARPKETILDNSRFSIAGDYFLNTGRGTATLPTGWGAFLAYDADESNGKGFDLRFEAG
                     YLNITKAPSSIEGFYGEAGFAWLYRIPKMPLQLVTSILPGLGYYNFKDASAQGNALKF
                     TAHAALGIEFPFMMKKTDRTDEIIPFLQLRGGVIYDNVLPIVHYGVYAGFAYKFGQVV
                     FKY"
     gene            19125..>19345
                     /locus_tag="PLF85_01225"
     CDS             19125..>19345
                     /locus_tag="PLF85_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18303.1"
                     /translation="MANKKIQLILLLAVLALLSACARKAKADDFQSFGGISYNAAPKT
                     ISAGLGLSIAVQAMDINFDGKMDGLYVCRT"
BASE COUNT         4198 a         5227 c         5384 g         4536 t
ORIGIN      
        1 acaactgcaa tgttgaagga catttgccag ttgcaagaaa atgcagcatt tcacttcgta
       61 gcttttggtt ggccgccgat cgcctcactg atacgaattc ttcacggggt acctggttgc
      121 ccgcgataaa gccattcagc agatctcggc tgacctgagc aatttctgcc gcacgtaggt
      181 attgtctttg ccagacgagc aggttttgca gggtttcgct gatgcgcagg aagtccgggt
      241 agttttcacc gctgcgcact tcactgctgg caaattcttt ctcgagaagg tctgcagctg
      301 cgacaaaatc cgatgactgg atccagaaac gtgcgatcgc gagagggtcc gattgatcgc
      361 cgtgtcgcga aaacaggaaa tccgtcaggg cacgggcaat gccgaggttg ccatagtaca
      421 ggtgctcctg gatttgaaaa aacaccgtcc gcctgcgcca gtagtttctg gctgaaggtg
      481 gaattgccgc gctgagctcg agaaaccgcg ttgcccgaag cccctgcgat ataccacttg
      541 tcgcacgcag cgtgttgctg ccacgcagca gccgcacgaa attatcgaga tcggcatcct
      601 tgtccttgag actttgagac atctggcttt ccgtacgcgc cacggtctct gccaggcgtt
      661 caaggccaac tttacccagg tcgtggcctg cgaggcctga ctcaatatag agctgcaggc
      721 ggcagagcag gtagtccctg aacgtatcgc ggttttctcc ccagggtaat tcatcggcct
      781 tcagttgtaa tggggagcgt ggccattcga agcggctgcg cagagaagcg taagcaatgt
      841 cgagagcggt ttcagcgcgc cggtaataga actcagcctg tgagacttcg cccctgaggg
      901 caaagtggcc gccgatggcc atctgcctct ggaattcgct gaacggacct tcctggaaca
      961 ggatgttcca gttgccctgc gccatgatcg aatcaatgag tcgtttgggt agggttgagg
     1021 caatacgcag cgcgtttttt tcccgccagg gaaccgcgcc agcctgaaaa aagaggaggg
     1081 aaaacaggca gagggatacc ttgaaaatac ccctctgcac cggaatctgt caggccgctt
     1141 tgagccccag cgccctggcc aacaatgaac gggcaatgac gcgcagcgcg agaacttctt
     1201 cgacaccttc gaagatgctg aaaacgcgtg cgtccacaaa gaggcgagat acttcatatt
     1261 cctctgcgta tcccatgcca ccatggattt gcatcgcttc gcgggtaacc cattctgaaa
     1321 ttttacagga gtaaaatttc acgagtgagg cttccatcga gccttcgtgc ttatccatga
     1381 ggcgcgcaga tttgtaagtc atctggcgca ctgcctgcgt aatcgcagcc attttcgcaa
     1441 tcttatgctg gttaatgccg taagaaagaa tcggcttgcc gaagacctta cgatccaggg
     1501 cgtactgcag ggccttttcc actgctgcct gcattacacc tgttgcgcgc gctgcggttt
     1561 gcaggcggcc accggcgaag ccagccattt gcaggtagaa acctttgccg agaccggctt
     1621 cgccgccgat caggttttcg tctggaacga agtaatcttc aaataccact tcataggagt
     1681 gcataccgcg gtagccgatc gtggcgatcg ccttgccgtg tacacgccca ccttcgggtt
     1741 gcttgtagtc aaattcgtgg tcgcgtgttg gaggtttttc tgccagcaga atcgacaggc
     1801 ctttatgctt gagcgacata tctgggttgg tgcgtgcgag cacgagcatt acgtctgcca
     1861 tgccggcaaa agtacaccag gttttgacgc cgttgatgcg ccagccgccg tcgacttttg
     1921 tcgcggcaac cttcatgcct gcgacgtcag aaccgtagtc gggttctgtc acagcgacgg
     1981 ccgacttctt ctcgcccgaa gcgagcaggg ggagccactt ctgcttctgc gcttccgtac
     2041 cgccggcaga aatagccttc gccacgattt ccgggcgcgt gatcaggctg ccggccacgc
     2101 cgacgctgcc acgtgacagt tcttccgtca cgaccgccat tgacgtgtgg tcaggatttt
     2161 cttcagactg gaaaccgccg tatgtgtcgg cgattgaaag accaaagcat cccatttcgg
     2221 cgaggccgtt aatgatttca tcgggaatcg tcagatcctg gcggtggacc ttttctgcaa
     2281 ggggtttaac ctggccttcc gcgaacttgc gaaaggtgtc cgccatcata gcctgatcgt
     2341 cgttgagacc ataagctccg taatctttgt ttgaagccag cgcatcagag attttttttg
     2401 caaaagcctc tgagctgtac tcggcaataa aaccatagac ttcagaagtt ttgagatcgc
     2461 tgtcaaattg ggcctgcgaa acgcccatct tttgggctgc ggcccttagc cggccgatga
     2521 tgtcctgcag tgcatctgca atgaaaaaca gcgaaaggtt ttcttcgatg ctgccgggcc
     2581 ctttaccgac tttcgccggg taagcaacca tttcatcgat cgcgaagatt tccgcagcta
     2641 cccaggccag gtcgtagttt gccacctggt cctggtccat cttatcggtt gagatgttat
     2701 tgttctcgaa agccttgtca gccaggtatt tttcgaactt accgtagaag ttcttaacat
     2761 tgctgacaat agccgtaacg ttggtgagcg ccatttattt gcccttttct acgcccacaa
     2821 tgtggacgac gtttgtggtt gctgaaccac cgatattgag ggccgccgca actttggcac
     2881 cttcaacctg attttcacct gcagtgcctg tgagctgctt atagaggtcg agcagctggc
     2941 gtgagccggt tgcacccacc gggtgtcctg caccaatcaa tccgcctgac ggattgaatg
     3001 gcagtttgcc gccgatacgg atgtccccgt tctcgatggc cttgtatgcc tcgcccggag
     3061 cggtcaggcc aaagtgttcg atcgctgcgt actctgaagt agtgaaacag tcgtgcgttt
     3121 cgaccaggtc gaggtctttc gggcctttga taccggcacg tttgtatgcg tcgacgatag
     3181 ccttacgtgt gtgcggcaga atgtaaccgc cttctttctt ggctgcgtaa gcttcttcgg
     3241 tcttgatgtc gaaaagcatc ggcgccgtgg cgtgacccca gcccatgatg tacggaatat
     3301 tcttcagctt caaaccatgc tctttggcat agcgcttggc gaacttctcc gacgcgagaa
     3361 atgtcacggc agcaccgtct gtaacctgcg agcagtcggt gatgtgaatg attccgccga
     3421 cttccatgtt gaagttacca cgcgagagag cgtgttgctt gttcatgtac cattcgcggg
     3481 tttgcgcgag cggattcttg cgggcatttt cgtagttgat ggcagaaatt tctgcgaggt
     3541 ggtcgtattt caggccataa agctcttcgt aaaccgtgcc gaggcgtcca aagagtttgg
     3601 ggaaaggaaa ctgtacgccc ttcgcttctt tatcgtacca tgccgctgtg ccgagaaagt
     3661 caccgccggt ttttgaatct accgatttca tcatttcgat accgacaacc atcgagagat
     3721 cgtagtggcc ggcttcgatt tccgtgcggg caacaagcgt tgaaatagcg ccagaagcgc
     3781 aggctgcctc gtggcggctg gtgggagcgc cgcgcagctt ttcgctgtag ttcacagtca
     3841 tcgcaccgag gtgcccctgc atggtataga gttcaccggc aaagttgcca acatgaattg
     3901 aacccacgtc tgccgggtcg atcttggttg cggaaaaagc tccgtccatc gcctcgtgca
     3961 tgagagcctg aatcgtcttg ccttctttcg cccagttgcg ggcaaagtct gtctggtagc
     4021 cgccgagaat atatacgcgt ttattttttg ccatttgtat ctccttctta tctgccaatc
     4081 agaaatttgc tgacgtctgc cacgcggcag atcttaccct tttctttgat agctttgagt
     4141 tgtgccggca ccggaatgcc agctttttca atgagtgccg cggtttcttt ggctcctcca
     4201 aacaggtcta cccagcctga aggcggcagc catgagaagc ctgaagacat cacgcggtca
     4261 ataccgtgaa taccgtcggc ctcaggggta acttcaccaa tacgcgcgaa gctgtacgcc
     4321 acgtaaccca ggataaagta acggacgatc ttcccttctt cgccaggtgc gttcttgatc
     4381 aggtcaatcg cgcgcgggta tataccgtcc tggatcgcct gtttagtctt ttcgacccag
     4441 tcgattttgt ctttcttggt tggagcaaag ctgaggtcgg caatgttcag ggtcgttttg
     4501 ttcttttctg cgtcgcggtt gaagtaaccg cctttgccct ttgccttgcg gccgagcatg
     4561 ccagcatcga tcatcttctg catgtatgcg ggcatcttgt aggtgtcgat gcgctcgtcc
     4621 ttgacgttcg caaccacatt gtcgacgatt gccttgtgta cgtcgagacc gacgaggtcg
     4681 atcgtcgcga gcggggcaag ggcgcggccg gtgtacggcc ccagcaggta gtcgatctta
     4741 tcgacgccgt atttttccgc gtggatcgct gcgtcgttca gcagctggaa accgatacgg
     4801 ttaccggcga atgccgctgt atcttcggtg atgatgttca cacggcgcag taccttctcg
     4861 cagaaatcgt aaacgaattc gcgggtgtct gccggtacat ccgggtgaaa aatcagttcg
     4921 ttcgcgggca gcttaccggg tgggttgtaa aagtgcgtac ccatgaagtt tttgcggaag
     4981 ttttcattgc ggccttcgcc catcttgcgg atcgagagac ctgatgatac agtgctgacg
     5041 attgcatcgg gttttttcag tttttcgatc ttggcgaaga attcgttctt gatcgccatg
     5101 tcttcggcga ggccttcaaa gatccagtcg cagctgggga tctctttttc gagatcgtcg
     5161 tagctcgcag ctttgatgta tttgcgcagc acatctgagc gtgcctggcc aactgcggct
     5221 tcgagccccg ctgtcgcctt gtcgagcgac cgggcgaaaa acacacattc tatgtccgcc
     5281 tgggcaaaga tacctcctga gagtgagccc atgttacctg aggcaccaag aaccccgact
     5341 tttttgatca cgcgtgttga tttcgccatg ttttctcctt taattgagcc aaagacagcc
     5401 ctggcagccg ctggatttta accctcaatg ctgacttcac ccgggtaata tgcagaattt
     5461 gcgcccgaaa cagcgcgaca agcaaaaggc tgccagagtg ggatttcgat cgacaagaca
     5521 gcccgttatc cccgggggga agcatgcgcc cgtttctgct catgattctt ctcgccgggg
     5581 gtatgctttc tgcccaggcg gaaaacagcg gcacgacgcc accccgtgtg gaatacgaaa
     5641 agcaggttcc accgctgccg ctgcgcaagc tggtcgatgc cgccgttctg atgctcgagg
     5701 cccgcgcata cgagcagttt atccgtacgt ttgtgcctga aagcgaccgc ggcaggtttg
     5761 aaaaagccta cacgcgcgag ggtggcgtcg actacgcgct gtgggggcag gagaagggca
     5821 gccgactcct caccgtactg cgcagcctcg cttccgcaga tgctgtcgtc agctctaccc
     5881 gggtttgcta tttttcgccg ggtgaggcga aaccgctgct ttcgtttatc caggtgcgca
     5941 gcatgtggct aatcgaaaac agtagccgct gcacagaccg ccggccggca aagccaccgg
     6001 ttgcaccagc cgaagctcca gcgccttagg tggctgcttt ttcaggagcc catcaggact
     6061 tacatttttc tctcaggccg ctgcgctcaa tgacaatgcg ggcgcggcag gttgtgccct
     6121 cgcattcgat gagtcgctgt ttctggtttt ctacccgaaa gctcgcgata ccgtcgtcgt
     6181 tcgagagtgc gccacaatgc cgcggccaat ggctcattgc atcgatcagt gcagcttcgc
     6241 gcgccattgc tgagcgctgc acctgtggca ggtcttcgcg tgcatgtcct tcaccaacca
     6301 ggtagagccg gtctgaacca ctgaacccct cgcgccaggt gctgctggcg cacgagggta
     6361 tagacagtag agcgaaagct ccgaccaggt aagaggtgat tgtctgcatg ttcattagac
     6421 gtcactcttg accaaacgat cgattttcag gatgttatcg acaaagcgcg caaagtcgga
     6481 aagatagagc tggttggttg cgtcagacct ggcctcgggt gggttgggat gtatctcggc
     6541 gaagataccg tcgagcccgg ttgcggctgc agcgcgtgcc agcggaaacg ccatgcgccg
     6601 gtcaccgccc gaaaccttgc ccccgccagg cagctgtgtc gagtgtgtcg catcgaagac
     6661 gacgtgcagg cctgcagcgc gcatgatttc gaacgagcgc atgtcgacga ccaggttgtt
     6721 gtagccgaac gtatagccgc gctcgcacag ggtgactttg tccgagcctg acttgcggta
     6781 cttgtcggca atctgcagcg cgtcttctgg cgcaacaaac tgtccttttt taatgttggt
     6841 ccactttccg gttttggcag cagcaacgac aaggtcagtc tgccggcaga gaaacgcggg
     6901 tatctgcagc atgtcgacat attctgcggc aacctcggct tcttcggggt tatggatgtc
     6961 agtaaggcag gggagtttaa aatcgtcggc cacttttttc agcagcgaca ggccctcgcg
     7021 cattcccgga ccgcgaaatg aatcaatcga cgagcggttg gccttgtcaa acgacgattt
     7081 gaaaaatatc agaattttct gttcatcggc aacccggcgg acctctttcg ctacggtgcg
     7141 cagcaggtct tcgctttcaa tcacgcaggg gccggcgatg aagaagggtt tgttgctgcc
     7201 accgattttg tggcctgaga taaaatcccg ctcggggttt tgggctacca tcattgttac
     7261 tccttgcgaa acgccatgcg gactgtgcaa ccggcattct tatcgaatcg agtggaaagc
     7321 tttttaccct cgtcgcgtga ctgtgggtat gctaccctgt ggcggttgcg cgcctttacc
     7381 tcagctgact cgcagttgcg tgcgtagcgc tctgcggcag gcagcaacag gctggcgtct
     7441 tcgtggcttt cgactacctg cagggtttgt ttgtcgagtg cataaccgtc agtgaagaat
     7501 tcagcatccg gtacctggta tctgcccgag gcacaagcga gcaggaaaat ggccgcgagc
     7561 aaactgcaga tgggcactgc atgctttttt atcgcggctc tgtccgctat gccgggcacc
     7621 ctaataagcc tcatgccgcc gcttcggtgt ggcgcatgaa ccagttttcg agttcgccag
     7681 cgggtatcgc cttgctctct gcttcagcaa gaatacgtcc ctcgtgcatg aggaaaattc
     7741 tgtcggcaaa ttcgtgcact tctttcagaa tatgactcga taccagtacc gcgtggtcct
     7801 gtctcagctc ggaaagcagg ctcttgattt cccggatctg gcgggggtcc aggtctgcaa
     7861 tcggttcgtc gagcagcagt acttcgggat tgtgtatgat tgcctgcgcc aggcccacgc
     7921 gcttgcgaaa tcccgcagaa agcgaagcaa tgtagtcgtg gcgccgctcg cccagcgcgc
     7981 agcgtttcaa cgccttttca atggcgtcgg tttcgccctg ctgttccgcc acgcgcagcc
     8041 ggtacatgta aacgagaaaa tcttcgaccg tcatgtcgtt gtacagggca ggggtctcgg
     8101 gcaggtagcc gatgtggctc agcagttttt cccgctctgc aatgatattc tcaccgttta
     8161 gcagcacctc gcccgtggtc ggcgtgatat agccggtgag catgcgtagc gttgtcgttt
     8221 tacctgctcc gttcatgccc agcagggcag aaatttcgcc gcggcggagc gtgaatgaaa
     8281 cacggtctac agcacgccgc gagttataat gtttcgagag attttcaacc gtcagcaagg
     8341 attacttcct catttggagc atcgtttccg ctgtcgcgaa atccccctgt acggtggaaa
     8401 tgatcgagaa attcttcatc ttttccagca tcgggccata agctttcagt gaagagcctt
     8461 gcttgctctt cattacggcc ttaaaaacct gctgcaggtc gagaaagaaa acaccctgtg
     8521 ccgactcgta aggaattttt gccaggcgct cgctcagtgt tgcactgccg cctttggtcg
     8581 ctttgagcgc gttagccttg ctgttgctga cgatcacctc gttttcgcga atcaggaaaa
     8641 caacggtcac agtctggcgc tttccattca ccatgttgcc aggaccgctg atactgacga
     8701 attcaccgtc ttccgcctct tcgaatttaa atttggtctt ctgtttcttg tttgcttctg
     8761 ccttcttcgc catcttctcg ctggctttca gcaggcgaac ccagtttttt gctgtgccgg
     8821 gtttaatgcc aaaggctccg tagagctccc acgcgcccac ttcgcgaact ttttcttcgg
     8881 tgggtatgtt cagcagctgg aagttatagt tgccgcgcag gctgttcagg atctgtgatt
     8941 cgaaatccat tcccaggttg gtattcgctt cctggattgc tgttgcgtat ttctcgccaa
     9001 ataccgggct cagggatttg aaaagatcga tcatgcccat cacgttcagt ttaatcaggg
     9061 tgtaattcac cgggttgcgc gccgctgtgt ctgaggcaag cggttcggtt ttcacctgga
     9121 taatgcgagg gtaaatcttg ctggtgtcgc tcaggtagcc ctctttatac ttgtaggaga
     9181 agtagaacga gatgcgctct gcattcgcca cgagcgcacc acccgaagat tgcaggttgt
     9241 cccggatttc tgcaacatac ggtgaattgt catcaatagc gggagctgtt tcgtcgccct
     9301 tattcagctc tttctgcagg ttacgcagca ggtcgctctg tgccttgagc gatgagacaa
     9361 tcagcttcgg attcaggtag aacgacatca tgggctgctt cttgccatta cgcgatagca
     9421 ggtgcgagcg catcgaggta taataagatg catcggtgat gggcgaagaa accttcgaac
     9481 gcattgccat gaccatttct ttcttgttgc tcacaagcag tgcgtcatcg gtgcgcacca
     9541 ggtaaccggg atcattttct gaaccgaaat ttaatacccg gcctgcctca acttctgtat
     9601 tgatgggcat ctggctttcg gtgatcttgg ccttgaggaa gtcatagaat ttggtgttgg
     9661 actggatcgg gaatatcatc acgaattcgg gcttcgaggg gttgccagcc gcctcgatct
     9721 ccatgttgat tgctattgcc cattgctcgc tggtcttgat gccgatttcc tccagttttt
     9781 gcgggtcgag catgttgacc cccatcttct gttcgaagtt ggccgacatc attgaccaga
     9841 taccttcgcc ggcaatgttt gctaccagtg tacgcagcgc ggtgaggctc tcggtaagtg
     9901 gagcgccttc aatatagatc gaagtatccg ccggtacgcg gtcagcggga cgggcagctg
     9961 caacgccgga aaccagcgtt accagaagta acagggaacg aatttgcata cttcttaaaa
    10021 aaaggcgccc taccggccga caagcgaaat aatggacaac atgattctca agccctgttt
    10081 gccgcatggc atttgagatt aagggatttc gcgggcgtcg ccgggcgaca atgcaggccg
    10141 ggcaaacaga gattgcgccc gcgacagcag aatccgagca actccccctc ggcatcaggc
    10201 tgcagcactg ggtgaacgaa aacccgcgcg ccattcgcaa cagcattttc gtgttggcag
    10261 gcattacggt cgccgtcgtg ctggttcttg tcctccgcgc agcagcaaaa gacgagcaca
    10321 gccgcaggct gcacgctgcg ctggaaaaat acgaacagat caaaattctt cccaaaggcg
    10381 atgcacgggc actgcagatg aaggagttcg ggcagagcct gaaacctgtg tgcgagcaga
    10441 ctattgcaac gctcgaaagt gccgcggcct gctttaccgc tggcagcgcg ctgcttgaag
    10501 gccgtgaccc ggtggctgca gccgcccttt ttgcccgtgc agcgtcaggt tatgacaccg
    10561 atgcgatggc tagtgttgca ggttttatgc aggcgcaggc gctggaaagc gcgcgtgatt
    10621 tcgaaaaagc cctcctggtc tataaagacc tggaaaagaa atatgcctta attaagaaga
    10681 ccgagatggc gctcttccac cagggacgta tgctctatta cctgggccgt tacgatgaag
    10741 ctgaagagaa gttcgcgaag ctgtcgcgcg acagtgaagg taaagaattc gccgccagca
    10801 gccgcaacta tatctcgctg ctcaatgctg agcgtgccca gttaaagaag taagttggca
    10861 gaagagaagc tgaaaatcgc gctccccaag gggcgcatga gcgatgaaag cctggagttt
    10921 ttttcgtcac gcggtctttg ctcgctcagc cgcacccaag acggccgcga actgattcta
    10981 cacgatgaaa aaagcaacat tgatttctac ctgatacgca gtaaggacgt cggtgcttat
    11041 gtagagcagg gcgccgccga tctcggcatc atggggcttg atttgctgct cgagcacaac
    11101 tttgatgttt ttatccctgc cgcactgcca tttggccaat gccggctgtc ggtcgcgtat
    11161 ccgaaagatc gggcagactg gcagaacaaa cccgagctgc gcgttgcaac gaagtatccc
    11221 cgcctggcga ccgaatactt tttccggcgg ggttttaaca tccgaaccat tgaactctat
    11281 ggttcgattg aaattgcgcc gctgaccggg ctctccgatg tgattgtcga cctcgtcagc
    11341 acgggtgcga cgctcaaagc aaacaacctg gtcgaagaag aggttatcct ggaaagtacg
    11401 gcgcgcctga tcatgaaccc gtctgcgctg gctttgggcc gtaagcgcct gatgcaattc
    11461 attgacaccc tgtttcaagt ctgattttca accaaaatgg ccgttcccaa gagaagactt
    11521 tcccactcac gcagacgtaa acgccgtgca caccacgcaa ttggcaaacc gaacctgcgc
    11581 ccctgcgcca attgtggcgc ttacggcatt ccgcaccgca tctgcactgc ttgcggctat
    11641 tacaacggac gccaggtcct gatcccgcgc gtcaaaaaag ccaaggctga taaagaataa
    11701 cctgtgcttc gcccgcacgg caaagcaagc cttgtcggcc agcacctgca ccacgacaac
    11761 tgttttgcgt gcggtgccct gaacaattac tctttccaca taccctcgac cttcgatgaa
    11821 cagaagggcg aggtatgctt tacctaccag ctgcgcaaag aacatgaagg cgctccgggt
    11881 tatgcacatg gtggcggcct ggcccttata ctcgacgaag cacagggagt tttgtgccat
    11941 cacctcggcc actttgtcat gacagatggg ttctctatca agtaccacaa ggcgacgccg
    12001 ctctataaag acatcaaggt aaccgcatgg cttacttcgg cccgctcgaa gcgcctttac
    12061 accaaggcgc gaatcgaact cggtgacgag ctacttgtcg agtccaaaat caaatggtat
    12121 atcatcccag agcggctgct cgagcgccgg ttcggcgaag acggcatggg tggcaggctg
    12181 aaagagcgcc ttgaaccgaa tcgcgtccgg gcaaaagaga tccgccgccg cttacgcgcc
    12241 gaaaaagccg gcaccgcagc aaaatagatc acaagccaac ggcctgcggt tctttaccat
    12301 ctttacagcc tgattcgcac tctccgatta ccgtatgcga atgctcgtag tcggctcagt
    12361 tgccttcgat gatatcaaaa cccctttcgg tgaaaaaaag aatgtactcg gcggagctgc
    12421 gagccatttt tccatagctg cagcgaatct cattgagccg aaaattatcg ccgtgattgg
    12481 cagtgacttt cccgagaagt atcttacgta cctgtctgac tttggaatcg atactgccgg
    12541 tattgagcgc agcagcgaca agaccttcca ctggtcgggt ttttatgaac acgacatggc
    12601 tgtggcgcat acccgcagca cagaacttgg cgcatttgcc agctttgaac caaagctgac
    12661 agaagaagac aagctccgcc aggtactctt tctcgccaac atcgaccctg taatacaata
    12721 caaagtactg aagcagatga agagtgttca gctcgtcggt atggattcga tgaacttctg
    12781 gatcgagagc aagaagaagg acctgtggaa ggtgatcaag gcagtggata ttctgtttct
    12841 gaacgacgca gaggtgcgca tgcttgcggg ggaagcgtca atcatcaagg ccgcgaaggc
    12901 aatccaaaag cagggccccg gtattgtgat cgtcaagaag ggcgagcacg gtgtcatggc
    12961 gatttcgcaa aaccacatct tcgtgagccc ggcatacccg accgaaaagg tgatcgaccc
    13021 gaccggtgcg ggcgacagtt tcgcaggcgg agtgatgagc tacctggtgg cgaactcgca
    13081 gccgggcaaa aagatatccg atgaactgct gcgtcgcgcg gtcgcatggg gttcggcgac
    13141 tgcgagcttc gcggttgaag gattctcaac gaccggtatt gccaaggcag acaaggccgg
    13201 cattgaaaag cgggtcgaag cgctgcggaa gctaagttct ttctgaattc agtcaccctt
    13261 cgactggctc agggtgacgc agtgtcatgg tgagcctgtc gaaccatgac aggttatttt
    13321 agctgatata gttcgccggc aaagcccgaa tccactcgtc tgtatcgatc gctatctgct
    13381 tcaccgtttc ggggtgcgtg ataatgccga ggtggccctg cttgtattcg gcaatccggt
    13441 ttttgggtga ttttccggca aggtgcccct gccgcacggt gtcgggagga gcgatttcgt
    13501 ctactgtgcc gaccaccgtg aagtgcggaa tttccaggtc attgcattcg agcgtgtaat
    13561 taatttcttt gtcgatcgac atgaagttgt ccccgtcgat cagcgagcgg atgaactggc
    13621 tgatcacgag catcgattct tcgcagaaca cgtcttccat catgaaatac cactcgggcg
    13681 gagtgatctg ctcgtagccg ataaactctt tgatcgtcgg caggcgatag gtgacgtcga
    13741 acgaaaccgt gcgaatcgaa gctgaaatac tgttgagaaa agtgaagaac ttgttgaggt
    13801 caattccacc cagggttgaa gttagtgtga agttcaccat ccgcatgatc gggtcggcca
    13861 gcgcattgcc ggtcagcatc ttcgccgaac tgcgaaacat attaaaccag gcattttcag
    13921 ccgcaatgcg gatatagatc ggcgaggtga tggaaacaat accgtcgatc atcgattctg
    13981 gctttatcgc cagctcgccc gatcttttct tgccgtgctt gtcgtaggcc gccgagtaga
    14041 agaggggaat caggccgccc atactgtggc caaagaccac gatcttctta ccggggtgcg
    14101 ttgccttgag ccactgcagc gcggccggaa aatcttccca gatgtagtta tcaactgacc
    14161 agtggtagtt gttgaagatc ttattgggga gcgtttcttt agagcgtccc ctgacgtcga
    14221 gggtgaagac cgggtaacca tgttcgagcg ccatctcttt cgccaggcga tccatgatcg
    14281 aacgattgca gaagaagccg ggcagcatca gcagaatacg gtcctgcccg ttcagatgct
    14341 cgggcaggta acgcttgaga gagatgttgt agccctttac ggtcggtacg atgtagttgg
    14401 tatcctgccg atacttctcg gagtagcggt tggagtagag taccatcgac ttgaccggtt
    14461 tttcctggtc aggtacgtgc gtatagacca gggtttccag gtttgccgtt gggtcgtgct
    14521 tttcgatctt gcgccgggcg ctgtaagcat cagcgccttc gaccagttct tctgagataa
    14581 taaagtcgat ggagcggctc agcgaaatca tttgctggcg cacatcgcgg cgctggtctg
    14641 cgctgaacga acgccggtgt ataccccaga gaactcccac tggtttgcgg tcgatgatga
    14701 tgggtatacc taccgcgtaa cgcacccctt tgggcgccag gcgcttcata gcaccatgca
    14761 ggcgctcttc tgatgcaata tgcagaaact caaccatggg cggcaggtca tgcgcgatgt
    14821 tatagagcga ctgccgcatc atctgtgcag acaggttctt ttcgtggcct atcgcaagcg
    14881 gtcgggtacg ggagataaac ttcttgaaaa cctccactcc tttatgttca gccagctcgc
    14941 gcagctggaa acgcgacgaa gaggccgtca cacgcatgta gtgcccgtcg ataatctcga
    15001 acacgctgta gtcgaagcag tggctgaagt cgggaaaggt gaaagaaaca accttgttga
    15061 cgaaggcact ctgtacctgt gaaagaaaat caaagcggct acgcggaatc ggcggtatat
    15121 aaagaaaacc gggaccgggt tcgtaagatt ggtccaacag ggtgcggggc cccatgctgc
    15181 gcttcatgtg cgatttttgc atgcccgtcg cagcgacaag ctgtgttatc ggaccgcccg
    15241 gctgcccggc tgcaattcga tatccgagtt gacagccggg tcaatttccg cactctgcct
    15301 cacatttaat gactacactt tatgcagcaa aggcctaatt tttccccacc cagatcccca
    15361 gacgggggcg gaggaggcca gggccgattt aatcgctttg ccaaactcag gcgcccgacg
    15421 cgcgaacacc gtatcaacga agacatcacc gaatctcagg ttcgtctcgt cggcgaaaaa
    15481 ggtacagagc ttatctcgac gcgcgatgcg ctcgcgcgcg cgaaggcgca gggcgtcgac
    15541 ctcgttgaga tcacgaaggg acaagaggtt cctatcgtgc gcctcgtaga ctacggcaag
    15601 ttcaagttcg aaaaggccaa gaaagaaaaa gaggcgaaga agaaacaaaa ggtcatccag
    15661 atcaaagaga tcaagatggg cccgaagatc gataccggag acttcgaccg aaagtgcaaa
    15721 gaagctatca gttttctcag tgaaggcgat aacgtaaaag tgaccatgaa attccgtggc
    15781 cgcgaaatgc agcacacggc gctcggcgtc gaaaaactga ccgacttcta caacaaggtg
    15841 gctgaatacg cgcacatgga caagaagccc aacctagagg gccgtatcat gagcatgacg
    15901 atgcgcagca agggagctaa acccaaggct gccgcagcgc cgtcagaaaa accggcagcc
    15961 cctgcgcccg aagcggcacc caagccccag gcttaatcag ccgctgagcg cgtccagttc
    16021 ggcgcttgtg aaccccgcgc gtaaccttgc ctcacggttg aggttaggtc ggggaaacct
    16081 ggtgccgggt attgcgtgct gcacatcttc agcaaaaata ccggtaccta cctcgtcgag
    16141 ggtgcaggca taacgaaacc agaaatcccc cgcatgcacg tgcgttactt catcgtcgaa
    16201 gataactttt agtgccgctg aaatctgctt tgccaacggg tcgtcaaaga gctgcatctt
    16261 gcgggcgagc tcagggtgtg cgtcaagccc attggcctcg aggtgtcggt gtgtcgccgc
    16321 catgcgctgc cgcagcgaat gcgcagagcg tgccatggcg tcatggatgc cggtgtgcac
    16381 gctgaagcaa ccgtagtgat aacccagctg cccgagcagt ttctgtagca tgacaaagtg
    16441 gcgtgtttcg tcgaaggtca ccttcagcca gtcttcatag tactgtggcg gcaaatgcct
    16501 gaaacggtag cagctgtcga gtgccaggtc gatcgccgag tactcgatgt gcgccagcgc
    16561 atgtaagaag accgcgcgtc cttctgcggt ccccagattg cggcgccgct cgacctgcga
    16621 gccatgcaca atgcggcaga tggcggcata cgagggagct ccaataggtt cggtggctgc
    16681 agcgtggtcc gcctgcactg cgtctgacgc aagagcctcg ttcagctcgc tgagcgcggc
    16741 gagcttgcgg ggtaaatccg attccgtgcg aatattctgc agtgcagtga agaattccat
    16801 gacaggttta ttcatctctg aattgacgga gacagcgtca aatcgttgct gccagcatgg
    16861 cccgcggcgt ccgcctctgt atcaccggca gcgaagtgat gaacggtttc gtgctcgatc
    16921 gtaataccca gttttttgcc tccgagcttt atgcgctcgg cctcgagctc gtcgaaagcc
    16981 gaattctgca cgatgacccg cagcacatca tggcaacctg gaaagaattt gccgccaccg
    17041 gcgatatcat tgtgaattcg ggcggactcg gccccacgac ggacgacctg accgtcgacc
    17101 tgctgtgcga gtggcagaaa gatacgcctg tctacgagcc acacgcagag aagcgcacgc
    17161 gttatttctt tgagaagcgt gcaaaattgc agccgaactc gtattcgctt gaaacggcgc
    17221 tgaggcaggc gcgcattcct tcacgcgcgg tcgcgatcaa gaaccccgtg ggccttgcgc
    17281 ccggtatcta tttacctgaa ctcaattttt tcgcattgcc gggttttccc gacgagatca
    17341 agggcatgtg gcccgaagtg ctgcgccgca tcgaagagca gaagactgtg cgctcggtga
    17401 ccaggattat ccctgtatgg ggtgtcggtg aaagccagct ttttaccgag ctgcgctccc
    17461 tcgcgccgga tattgacaaa aaagtttcgg tcggcgtgca cgcgctgcca tggggttgcc
    17521 ggttgttcct gcgggcgaaa gccggtgacg ccggcagcga agctgcagtc gcgcaatttg
    17581 ccggtaccct ggaatctcat tttgccggcc aggtagtcga cgatccgctc ggtggagtca
    17641 ttgcgtactg caaagagaag cagttaaagc tggctgtcgc agaaagctgc accggaggct
    17701 tagccgcaaa acagatcact gacatcgcgg gggtctccgc catcttcacc ggcggggtag
    17761 tgagctatgc caatgaagcc aagacagcat tggtcgatgt tccgcaagac ctaattgtgg
    17821 cgcacggggc agtgagcgcc gaggtggcgg cagcaatggc gaggggggtg tcccaaaaac
    17881 ttgagactga cctttcgctt tcgtttaccg gcatcgccgg cccggacggg ggcagtgaat
    17941 caaaacccgt cggcacggtg tttgtcggtc tgcacgatgc ccgcaccggt aagacgctcg
    18001 ttgcccgttt cttgtttccg ttcgggcgtg agcgctttcg caatgcaacg gtggcgacgg
    18061 ggtttatcgc cctgtggcaa tacctgcacc agccaaacgg tggcggggtc ttcccgcttt
    18121 caacctttca ctcggctctc tagtatggac gctaggccat actagagaga tctaaatgaa
    18181 cacttgttta caaagtgaca aaatcgtcag ttttttcgcc ttttttggca cagcgatcac
    18241 cctgttaacc atataacaat ggggtaatcc ctatacatgc gccagaagtc tgtccatacg
    18301 attgttttct tttctatgat tggtttcctc ttattagggg gggcttctcc cgttaggcag
    18361 gcgccgctct gggcgcagga ttcagagagt ttcttcgatg atgaactgcc cgaagaaaag
    18421 ccgaaatcgg gcgacctgaa atccaaaccg attgaaatcc gcgaggacga tgaggccgag
    18481 cgcaagaagg cgttgaaaaa gacgaatgag aagggcgagg ttgttctgcc gaaagtagag
    18541 aagcctaagg atgacggccc catcgagcag gtggctccgc tttctgcacg gcccaaagag
    18601 acgattctcg ataattcgcg cttttctatt gccggtgact atttcctgaa tacaggccgc
    18661 ggtacggcca ccttgcccac aggctgggga gctttcctgg catacgatgc cgacgaatca
    18721 aacggcaagg gcttcgacct gcgttttgag gcgggctatt tgaatatcac taaagcccct
    18781 tcttcgattg aaggctttta cggcgaggca ggctttgcct ggctctaccg tataccgaag
    18841 atgcccctgc agcttgtgac ctccatacta ccagggctcg gctactataa cttcaaagat
    18901 gcgagcgctc agggtaatgc gctcaaattt acggctcacg ctgcgcttgg tatcgagttc
    18961 ccatttatga tgaaaaaaac cgatcgtacc gacgaaatca ttccctttct gcagttgaga
    19021 gggggagtca tctacgataa cgttctaccg atcgtgcact acggggtgta cgctggcttt
    19081 gcatacaagt ttggtcaggt ggtattcaaa tattgagagt ggttatggca aataagaaaa
    19141 ttcaactcat ccttttgctg gcggtactcg cactgctgtc cgcctgcgcg cgaaaagcca
    19201 aggcagacga cttccagtcg tttggaggca tcagctacaa cgctgcgccc aagaccattt
    19261 cagccggtct gggcttaagt atcgccgtgc aggcgatgga catcaacttc gatggcaaga
    19321 tggacggtct ctatgtctgc cggac
//
LOCUS       DAOQJF010000011        18904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1342_length_18904_cov_5.228394, whole genome shotgun sequence.
ACCESSION   DAOQJF010000011 DAOQJF010000000
VERSION     DAOQJF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 18904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18904
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1342_length_18904_cov_5.228394"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLF85_01230"
     CDS             complement(<1..259)
                     /locus_tag="PLF85_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18304.1"
                     /translation="MKHRIPGLLFSILPLLVFLPVRAAEMPVQYYYDQAKNLEKSGDT
                     IRAADMVREALRRNGDHVPSLLLLARLSLQNRDFRVAQDLAQ"
     gene            complement(275..2425)
                     /locus_tag="PLF85_01235"
     CDS             complement(275..2425)
                     /locus_tag="PLF85_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE18305.1"
                     /translation="MRSPPALPAFLSRYRTWFIALALLLGAGVSWAVLYPTLGTRQNL
                     AEEEFQKAWLLYQDRKFDAAIEKFSQALVINPQFHWARRFLAQAYYLSGQTAEAIEEF
                     ETLARALPHDLTLRARIEWLSLPADGVASASEPSEFLRVVPRMQGYRYNRPTFIGSLH
                     SDQLAVLSLGNFEIGNMVSYSAQGEPIENRHRVSGRLNYPMAFAQNEQEVWITDFRED
                     KIHRLNKNTKRYLSYLFNPDAIGQSGEGELQFRAPAGICHREGEFIVADSGNNRLQRI
                     GDNGRFIAFIQRPAEGDPMQSPFGIWCDEDSIWLTESGAARVTQLDRYGNVVAEHSPA
                     GLSKPRHISWDAEQQQFLVADESQGVLRLSKSGEILGKISGYTNPEGRFITFARPYAA
                     AYDPFRNLYVADYGSSEVVQFAPASEKFGQLYLQVEKISAAKFPMVGVYVTVSTGPAG
                     AQSDTGIPRYLTELKASDFRIFENDTSVGNLGADYLQRFDDVLNSVILVSRSERMREY
                     ESQQQWVLDHLLKQIREKDRMRVLSHGSDVRAESDFINSRLKILQTVKAATSENNATK
                     AALGSMSAGLYDAITELITREGKRAVIYLTDGEADDDGLSPYSKDRLIDYARANHVPV
                     YVVSFEHPENLKSPEPKEALVALAKKTGGAYYRALEIDPRIDSLLRKQKEVRYLLSYQ
                     SMVRKQTTGQYVDMRVAARFRDRKGLELSGYFVP"
     gene            complement(2473..2793)
                     /locus_tag="PLF85_01240"
     CDS             complement(2473..2793)
                     /locus_tag="PLF85_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18306.1"
                     /translation="MKDVVLYVFFCLLYGVLAVRWVPAEGLVGDVLGWQALGEAVLLF
                     AGVATLARWTVLAFKPGPERLKAGQQIVGVEEPEADRVIDVVNPVALKREEKKPRLKK
                     RRLF"
     gene            complement(2809..4452)
                     /locus_tag="PLF85_01245"
     CDS             complement(2809..4452)
                     /locus_tag="PLF85_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18307.1"
                     /translation="MKKALRILKKVLLGFGILIVVIMVGGFIALKAVVTKDFIATKIE
                     ENINGRVEIKDISVPIWAAFSGITIDGFKIGYRDAEVGKPMESRTPMKNEVIGFKQFN
                     FQVAIGALITSFGKKFELKSLLFTEPVAHIVLGKNGGNNLQPLLTKPKTKEELAKEAE
                     EAKKAAEEKKAAAKKEAEKPKEPEKPFDFRELPTEIKMGKIGIEGGYFTVNVEALGQT
                     LKVSRANVLLRDIHLDPKNLEKHNFVGLTTQFDLELQESKGKGIKSFKIIFDLQGGIN
                     PINPKTGGPAESVTLRTGLKKGTFVTGLSIFEKLKDQTENLNKIGIKLGFLAETQTLT
                     RDAIATIYYAGGVVTIKEPPALITNDFEFRLSKEDYINVKSLDHHFRGDLSLAAQHTK
                     TVEGDLDKVIGSAVTPALDQVPAGPVRDQVKAALAPEKIRASVLAPAMKNGLITFGID
                     SSATISSPNVKVVSPQFPDLKSLIANETKKATSNLGDMVGAQVDALKGKAMAEGKAQV
                     DAAKAKAQAEAERQRKAAEEEAKRKAQQELMKSAPKLPF"
     gene            complement(4486..6273)
                     /locus_tag="PLF85_01250"
     CDS             complement(4486..6273)
                     /locus_tag="PLF85_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNE18308.1"
                     /translation="MHSHSILMNMLVFMAAAVVAVPLATRLGLGNVLGYLIAGAVIGP
                     WGLKLISNVSDILAFAEFGIVLLLFIIGLELRPGRLWNMRRDIFGFGTLQIVLSMVAL
                     GVPLAMLGLSTSQIGILAFGFSLSSTAFALQILTEKHEINTQYGRASFAVLLFQDIAV
                     VPVMIMLPILTQSSGQGNSGFMSLLPGLAALAAVLTGARFAVRPFFRVVVRAKSRELF
                     TAASLLLVFSIAALMEAAGFSMALGTFVAGVVLSESEYRHELEANIEPFKGLLLGLFF
                     IAVGMSMDFGVLLRYPLLVPLAVLALLALKIFAIWSAGNLFRYDAEVSRNIAFLICQG
                     GEFAFVLFKLSSDKQLLPPETVSIANVVVALSLLATPLVYLLNARYLRVRFAASEWKY
                     DDMPSEHNPVLIAGFGRYGQIIGRMLRLNDIRFTALEIDYEQVQAVGRFGNKVYYGDA
                     SRIDLLEAAGAAHAKIFVIAVDDMEASVRIAEIVTHNFPHLKVLARARNRAHAHSLMD
                     LGVTDIRRETFASGLETAHAMLRCLGFTEKKASKMISVFREHDEAMLVEQRQLRDNEK
                     LFIDYTNRAGLQLAEVFRRDLVGEEKEKD"
     gene            complement(6407..7141)
                     /locus_tag="PLF85_01255"
     CDS             complement(6407..7141)
                     /locus_tag="PLF85_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNE18309.1"
                     /translation="MERGRKNLRRIAMKVNIWHRRVALLSAIFLAVTAITGILWAYAP
                     HLYFKDGYLKKKHVQGAPALSGARLSPQDAIRLTETVGKVNPAESVVLRAEGGRLVYE
                     VVRREGKAAHSQLVDAISAEKLSPLDKQMAAAVAAEYVLANPTIKDAEIIDHYRHRSG
                     KIVASVYRVRFDAPGNPEIIIDRNSAAIIEESDDARAFHFWVMKLHQLQFFGTKKELT
                     LIPGLALVVLVCTGMVIWWRRYRAMQ"
     gene            complement(7143..7838)
                     /locus_tag="PLF85_01260"
     CDS             complement(7143..7838)
                     /locus_tag="PLF85_01260"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HNE18310.1"
                     /translation="MKRLLTLLAAALLLPGILSAHYIWIEREGGTARVYYGEIQDGER
                     ETSPGKLDKITGLRVYTADKKGQNKEVKNTKTAEFFVAEAGKAEFVLADSLDVPVADM
                     KKYNWGMVKPMYYARHGAQSANAEVRPAQTLDIVPVAGGQNVFQVFFNGKPLKGEKLM
                     VYAPNTWAKEYKTDAEGKVKLETPWAGQYVLEVIHTDKTPGKFEGKDYESVRHRATYT
                     FKKELPHPPAPSP"
     gene            complement(7841..9988)
                     /locus_tag="PLF85_01265"
     CDS             complement(7841..9988)
                     /locus_tag="PLF85_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNE18311.1"
                     /translation="MRINIVYGVAAVSAAAVFAQPVQKPQNKKQPAPTAVNPRQATTA
                     APAVPAEAQLDEVVVQANSRVTGSKTDVPVHDTPASIVVVPKEIIEDQQSVDMNQMAR
                     NVSGLTTTLAGGYGFSDSFMLRGLNMRFMRDGLPDGNTWNGYMRTNTDVESVEVLKGP
                     GSAIYGRAEPGGVVNITTKKPMDTFGASLETMGGFWNTYRGVLDVGGPLGTQALPARL
                     NASYYHTDGYRGLKRTVGEFLPSFTWKINDKHSITVDYDYRNIKARPDNYGILFGADR
                     SLMSVSKSNTYYSPFNTATQQVHRAVISHEGVFSPKFKLRSALIYDNRDLYLMRNAGG
                     AVNAAGTQMTGRTAREQGDKSSDIVYQTEAVLKFLTGEMAHTLLAGIEAQSYKVNTWR
                     TDYNLPAITDLVNPVVLERSAYDYAAIPSFDRQITNQTVSAYAQEQVAVTDYLKLRAG
                     IRLDHAEIKDDGWQGAAKTQRTIQMARDLISWQTGAVLQPWKPVSFYGGYSEGRFMAI
                     QTESTSISVEPERSSQIELGKKLNLFKDKLQTNLAFFRTTRENYYVTDAAGVVSPVGK
                     RLTDGIELDIMGNPLKGLWLTFSSAFYDAKIKSTERVSGVNTEGKAPQYVPEYTGNFW
                     ITYELQSGILKGLGFGGGPTVKSGLYVDALNLLRVPGYVVADAVLFYKQQNWQLQANF
                     RNITNEVYFATPTFSGALPGEPFNFTISAKARI"
     gene            complement(10054..10671)
                     /locus_tag="PLF85_01270"
     CDS             complement(10054..10671)
                     /locus_tag="PLF85_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNE18312.1"
                     /translation="MPSLTKSLSKFTSASLLASVALHASAGGSFYLWVTFGRNMHLHD
                     VELSAAPLVPRAANSAVPEQVNDDWFLVQKNRRQLKPAEQTKETPREASVCQGKCNET
                     TGFGDAIQATDAHRKPRWVGNFITANDYPLLAAQQGKDGRVVLSVIIDSEGRVRDAQL
                     LEGSYDALNEVALRKVRDAVFSPAYDKDGNAVACRVRVPIRFELR"
     gene            complement(10675..11073)
                     /locus_tag="PLF85_01275"
     CDS             complement(10675..11073)
                     /locus_tag="PLF85_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNE18313.1"
                     /translation="MHLDSEEDGVVTGINVTPLVDITLVLLIIFMVTAHFVTDTGVKV
                     NLPKAAAAEVNPTPALTLTVAKDGELQLMGQKVDLNGLKANMSREAQLNPGVRVVVAA
                     DEALIYKDVVKILDLIKQAGVTRVALSSEK"
     gene            complement(11060..11737)
                     /locus_tag="PLF85_01280"
     CDS             complement(11060..11737)
                     /locus_tag="PLF85_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE18314.1"
                     /translation="MNEIFKKAALLGDVWVLWLLLAASILSVAVMFDRWRLFRQNRID
                     FAGFFSGLRELLEQGKTGNALAYCTQFAAMENRIAAEGLRNFTKGAHSVEEAMKSALV
                     IERGNFEKNLIVLSTMGNNAPFIGLLGTVLGVIKAFNDLGAAGSAGVSVVMTGISAAL
                     IATAFGILLAIPAVIANNYFQTRLKRATANTQSLIHLMQVYLRAQPQGDFVSSAIRNT
                     EANLAPR"
     gene            complement(11884..12582)
                     /locus_tag="PLF85_01285"
     CDS             complement(11884..12582)
                     /locus_tag="PLF85_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE18315.1"
                     /translation="MWRMYLTQFWLMLLCALALPWLVITFRRPVNTGLFMYLYAVVAQ
                     RLSGIRIQLQGAEKVLREPAIYVLNHQSSLDGIPLGMLRIPRLAIIGKKEVAFIPFIG
                     WAFWLAGNVLIDRADGKKARGQLDAGVAAIKNRRVSIAIFPEGTRSKEVGFLPFKRGA
                     FQMAIEAGVPVVPVVIESLKKVIDTAAKRYGGMLRVHVLAPVATAGLTPKDAPTLAAR
                     VQALMQSDFDSMQK"
     gene            12701..13258
                     /locus_tag="PLF85_01290"
     CDS             12701..13258
                     /locus_tag="PLF85_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18316.1"
                     /translation="MAQAHEILDVRKNGAFIFSDILLIDYRQQKLGSLLSEIKMQSHQ
                     LDRMLKKYTHDDERLLGQEKANVVNVLHMIIRNLAALYLRLFDLEHSAEYHSEIEVSE
                     GKDGFLIKGKMDADEVNIRFSLKQWTDEYLDAALADLFKDFQDAAKDHVITLAEKVEL
                     TGRIARILVKTMQAFYLMRSGAVFR"
     gene            complement(13259..14179)
                     /locus_tag="PLF85_01295"
     CDS             complement(13259..14179)
                     /locus_tag="PLF85_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNE18317.1"
                     /translation="MQSRFVRELIVGCAVSFLVVTSVAGSNDSTRKGLRVFISADMEG
                     VAGVVNGAQLAPGEFEYEKFRRLMTAEVNACIEGAFAAGATHVTVADSHGNGLSLLPE
                     ELNPRARLVRSWPRPLAMVEGIDQGYDAVILLGYHASIGTAGAVRAHTMSSKRIYGMR
                     LNGVHTSEALLSAAVAGHYGVPVVLISGDDKTIEEARRTISEKITGVVVKRAIGYHSA
                     DNLSPQQARLAIQNGTKEALANLSRYKAYATPKPVKLEIAFKNMMNAEVLSLLPVVER
                     VDGSTIRFTGKDMPEVMRFIEFVGQYSTAE"
     gene            14399..17164
                     /gene="greA"
                     /locus_tag="PLF85_01300"
     CDS             14399..17164
                     /gene="greA"
                     /locus_tag="PLF85_01300"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNE18318.1"
                     /translation="MSETETVTETQTSGAESLEARIQSQFNEEKWTRLSAKDVSISRF
                     KILEQIFDEVKKQNKQDLLRSESQAHLAEYDQSIAAHYFLGMMALEKNIPDEIVHLKT
                     LLDQFQEASKWAVVDYLSEKMLAVTKTRTILRARANALEKLGKHKEAIPVLEQLAVLD
                     RKNPDVALKYADAIIGDDMEKALQFYKQAAEAFARTLQFEKLKTTWNRLIELLPDDFN
                     FYKKIERILSGHRQREMIAELYVLLANYFIKKEDHDKVMALSKKILEYNPGYVRFKNE
                     LIRTYREKYKSHSLLEDFIKYSGLLDARKSLQSSILNFETNIVFDKGNYVFHRSWGVG
                     KITELNTAEMVIDFKDKKGHKMDIQMALKSLKPLREEHFWVMQFEKPEEMAKLFKEDI
                     TQFFKILLQSFGGKLSLADIKTELTDKYVPLSQWSKWWGKVRPELMKDALIGISAQKK
                     DMIELHETPITTSESAIEKFQAAQGFEDRVQAFIDVLKSPEGNLEAIEFIAPHFRETL
                     KSLDPNTRLKSLWLLEMATEALADDEPLFSKEQQAEIIQAFKSRTPNDLTELLQEIRQ
                     SELKRHALKFVKRHHNDWKRLYLEMMFETPVKLHKSILSDLSAANATDEITEFVNRLK
                     KDIKENAEVFLWTTKSVIAGQIELPAGQSQELLLGLFRMLKSIPKIEPKGSKLRNSVR
                     DLFFSNGASELMAVISREAKDSVRRFASLLKDVPFFNDGEKIQIVSQLREINPAVFEE
                     QQDESDLPEQTESLAQKLEKSGATVASADAIEAMKARLDQIINVDMPKNSEEIGLAQE
                     KGDLRENSEYKAALENQTILQATVTQLEADIKSVQQLSAEDVDTSVVSIGTRVKLRDS
                     ASGDIFVYSILDQWDADMDKGIISYKSPLGKVLIGARKGASATFNAQKLEVIGIEKAI
                     DNAGRLI"
     gene            17184..17966
                     /locus_tag="PLF85_01305"
     CDS             17184..17966
                     /locus_tag="PLF85_01305"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNE18319.1"
                     /translation="MNALSRLVFALVLTAGALSAQGKTGIGGTAFEIMDRVFQQLQYA
                     EGHQKLQLTITTREGKSVVYKGSLYQKQNASFFVFDSMTRGTVLKLLYNDQGQNIYAY
                     QTHDKRLFHKKYLDRFESVLGSGFAYLDIANASFIDNYQQKTKGTEKNDEGEFTVIEN
                     IPLDKGHYGKLIVLVDPKKDNRVKRIDYYDSAMVLMKTMTFTYGMLSVKEEKNLTREI
                     EFPIRWDMADVSRGTVSTLEFFSVDKTARLDASLFKKENIEK"
     gene            17970..18701
                     /locus_tag="PLF85_01310"
     CDS             17970..18701
                     /locus_tag="PLF85_01310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNE18320.1"
                     /translation="MSVAPTPLRSLQREHALLLLAQEEFTHLVERGKLPLPARHLEHF
                     SRVMRRHHRWRALAGNGMGTLAVCEVQKDTVVLTAEGIQEFPRTSPRVTLVQAWPKPK
                     ALSLILQKAAELGASELVLVTSDHAAHPAEKAERMEAILENACMQAYNLYKPSVSLRA
                     LADHANFLDTMAFFGDIQGSEKIRNLAMPAGQKATFVNGPEGGFSTRETDWLRERATG
                     VLLSENVLRSETAAIIALGHFCQSP"
     gene            18704..18901
                     /gene="thiS"
                     /locus_tag="PLF85_01315"
     CDS             18704..18901
                     /gene="thiS"
                     /locus_tag="PLF85_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNE18321.1"
                     /translation="MNGEKFDHASLAPAYDIQSLLRCLSLSERRVAIEVNGELLSNSQ
                     YAGKQLAENDNIEIIHYVGGG"
BASE COUNT         4404 a         5282 c         4974 g         4244 t
ORIGIN      
        1 gctgcgcaag atcctgcgca acacgaaaat ccctgttctg cagtgaaaga cgcgccagca
       61 gcagcagtga cggcacgtgg tcaccattac ggcgcagcgc ttcacgcacc atgtcggccg
      121 cacggatggt atcaccactt ttttcaagat tctttgcctg gtcgtagtag tactgcacag
      181 gcatttctgc tgcacgcaca ggtaggaaaa ccagcagcgg aaggattgaa aataagagac
      241 ccgggattct gtgcttcatt gcttttgccc gctcctaggg tacaaaatac ccactcaact
      301 caagtccctt gcgatctctg aacctcgctg ctacacgcat gtcaacgtac tggccggtag
      361 tctgcttacg aaccatgctc tggtagctga gcaggtagcg gacctctttc tgcttccgca
      421 atagcgagtc gatgcgcggg tctatttcca gggcacgata gtatgcgccg cctgtcttct
      481 tcgcgagcgc cacaagggcc tctttcggtt caggggactt cagattttcc ggatgctcga
      541 acgaaacaac atatacaggt acatggttcg cccgtgcgta gtcgatcaac cggtccttgc
      601 tgtagggaga caggccgtcg tcgtccgctt cgccgtcagt caaataaata actgcccgct
      661 tgccttcacg cgtgatcagc tctgtgatcg cgtcataaag gccagcgctc atactgccaa
      721 gagccgcctt ggtcgcatta ttttcagaag tcgccgcctt aaccgtctgc agtatcttca
      781 ggcgcgaatt gataaaatca ctttctgcac gcacgtcgct gccatggctc agaacccgca
      841 tgcgatcctt ttcgcgtatc tgcttcaaca ggtgatcaag cacccactgc tgctggcttt
      901 cgtactcacg catacgctcg ctgcgcgaga ccaggattac cgagttcaat acatcatcga
      961 agcgctgcag gtagtcggcg ccaagattgc caacgctcgt atcgttctcg aaaatgcgaa
     1021 aatcggaagc tttgagctct gtcaggtagc gtggaattcc cgtatcgctc tgcgcgcccg
     1081 cagggcccgt cgagacggtt acatacacac cgaccatcgg gaatttcgct gcactgattt
     1141 tctctacctg caggtagagc tgcccaaact tctccgatgc gggagcaaac tgcacaacct
     1201 cgctcgaacc atagtcggca acgtacaggt ttctgaacgg gtcatacgct gcagcataag
     1261 gtcgggcaaa agttatgaac ctgccttccg gatttgtata acccgaaatt tttccgagta
     1321 tttcaccgga tttgctcaag cgcaacacac cctgcgattc gtccgccacg aggaactgct
     1381 gttgttcggc atcccaggag atatggcgcg gtttcgacag tccggccggc gaatgctcgg
     1441 caacaacatt accgtaacga tcgagctgtg tcacccgcgc agctccgctt tctgtcagcc
     1501 agatcgaatc ttcatcgcac cagatgccga agggcgactg catcgggtca ccttctgctg
     1561 gtctctggat aaaggcaatg aaacgaccat tgtcgccaat ccgctgcagc cggttgttgc
     1621 ctgaatcggc cacgataaat tcgccttcac ggtggcagat gccggccggc gccctgaact
     1681 gtagctcccc ctcgccgctt tgcccaattg cgtcgggatt aaacaggtac gagagataac
     1741 gtttggtatt cttgttaagc ctgtggatct tgtcttcgcg aaaatcggtg atccagactt
     1801 cctgctcgtt ctgcgcaaag gccatcgggt aattcagccg gcccgaaacc cggtgccggt
     1861 tttcgatcgg ctccccctgc gctgaatagg aaaccatatt accaatctca aaattgccca
     1921 gcgaaagaac tgccagctgg tcagaatgca gcgaaccgat aaaggtcggc cggttgtaac
     1981 ggtaaccctg catgcgcggc acgacgcgca gaaattcact cggctcactg gcactggcaa
     2041 caccgtctgc gggcaggctc aaccactcga tacgcgcgcg cagcgttaga tcatgcggca
     2101 gagccctggc aagagtctcg aactcctcga ttgcttcagc tgtctgacct gacaggtagt
     2161 aggcctgcgc caggaagcgc cgggcccaat ggaattgcgg gttgatgacg agcgcctgtg
     2221 agaatttttc tattgcggcg tcgaacttcc tgtcctggta gagcaaccag gccttttgga
     2281 attcttcttc agcaagattc tgcctggtgc caagcgtagg ataaagaacg gcccatgaaa
     2341 cgcctgcccc caggagcagc gccagggcaa taaaccacgt gcggtaacgc gaaagaaatg
     2401 ctggtaatgc cggaggcgaa cgcattaaag atgcgacata ataccggccg ctcaggcgag
     2461 cgtcaagttt aattaaaaga gacgccgttt cttcagccgg ggcttttttt cctcgcgttt
     2521 cagcgcaacg gggttcacca cgtcaataac gcggtcagct tcgggttctt ctaccccgac
     2581 aatctgctgt ccggccttga gccgttcagg gccgggcttg aaagcaagca ccgtccagcg
     2641 tgctaatgtt gcaaccccgg caaacaacaa gactgcctca ccgagcgcct gccagcctaa
     2701 cacatctcca accaacccct ctgcgggaac ccagcgaacc gcgagtaccc cgtagagcag
     2761 gcagaaaaag acgtagagca ctacgtcttt cattgagtca ccagacaatc agaatggcag
     2821 cttgggcgca cttttcatca gctcttgctg cgccttgcgc ttggcctctt cttctgctgc
     2881 cttgcgctgg cgctctgctt cagcctgggc ctttgctttg gctgcatcga cctgcgcttt
     2941 accttctgcc atcgctttgc ctttcagcgc atcgacctgg gcacctacca tgtcgccgag
     3001 attagaagtt gccttcttgg tttcattagc gatgaggctc ttcagatcag gaaattgcgg
     3061 tgaaacaact ttgacattgg gagaggatat cgtggccgac gaatcgatac cgaaagtaat
     3121 caggccattc ttcattgccg gagcaagcac gctggcgcgg attttctccg gggccagggc
     3181 agctttgacc tggtcgcgca ccgggcctgc tggcacctgg tcgagagccg gtgtaactgc
     3241 cgagccaatt accttatcga ggtcgccttc cacggtcttg gtgtgctgcg ctgcgagcga
     3301 caggtcaccg cggaagtggt gatcgagcga tttgacgtta atgtaatctt ctttcgacag
     3361 gcggaactcg aagtcattgg taatgagtgc cggcggctct tttatcgtta ctacaccacc
     3421 agcatagtag atagtagcaa tggcatcacg cgtgagggtc tgcgtttcgg cgagaaagcc
     3481 cagcttgata ccgattttgt tgaggttttc cgtctggtct ttcagtttct caaagataga
     3541 aagcccggtt acgaaagtac ctttcttcaa cccagtacgg agcgtaactg actcggctgg
     3601 tccgcctgtc tttgggttga tcggattaat accaccctgc aggtcgaaaa taatcttgaa
     3661 agacttgatt cctttgcctt tgctttcctg taactcaagg tcaaactgcg tggttagccc
     3721 tacgaagttg tgtttctcca ggtttttcgg atcaaggtga atatcgcgca gcagcacatt
     3781 cgcgcggctt accttgaggg tctggcccag tgcttcgaca ttcacggtga aataaccgcc
     3841 ttcgatgccg atcttgccca tcttgatttc agtggggagc tcgcggaaat cgaacggttt
     3901 ttccggctct tttggttttt cggcctcttt tttcgctgct gcttttttct cttcagcagc
     3961 tttttttgct tcttctgctt cttttgccag ctcttctttg gtctttggct tagtcagcag
     4021 cggttgcaga ttgttgccgc cgtttttgcc cagtacgatg tgcgcaacgg gttctgtgaa
     4081 gagcagagac ttcaactcga atttcttgcc gaacgaggta atcagggctc ctatagccac
     4141 ctggaaattg aattgcttga aaccgataac ttcatttttc atcggagtgc gcgattccat
     4201 gggtttgcct acctcagcgt cgcggtagcc gattttgaaa ccgtcgatcg tgataccgga
     4261 gaaagctgcc cagattggaa cagaaatatc tttgatttct acccgaccat tgatgttttc
     4321 ttcaatcttg gtagcgataa aatcttttgt aacgactgcc ttgagagcga tgaaaccgcc
     4381 gaccatgatg accacgatca ggataccaaa tccaagcagc acttttttca gaatgcgcaa
     4441 tgcttttttc atatggggga gatagtccca tacttttcgt acaggtcaat ctttttcctt
     4501 ttcttcgccg acgaggtcgc ggcgaaaaac ttctgcgagc tgcaggcctg cccggttcgt
     4561 gtagtcaata aacagttttt cattgtcccg caactgccgc tgctctacca gcattgcctc
     4621 gtcgtgttca cgaaacacac tgatcatctt tgaagctttc ttctcagtga aaccgaggca
     4681 gcgcaacatc gcgtgcgcag tctcaagtcc agaggcaaaa gtttcacgcc tgatgtctgt
     4741 tacaccgaga tccatgaggc tgtgcgcgtg cgcacggttg cgcgcgcgcg cgagtacctt
     4801 gaggtgcgga aaattatgag tgacgatctc ggcaatgcgg acgctcgcct ccatgtcatc
     4861 gacggcaatg acgaaaatct tcgcgtgcgc cgctccggcg gcctcaagca agtcgattcg
     4921 gctggcgtcg ccgtagtaga ccttgttgcc aaagcgaccg acagcctgga cctgctcgta
     4981 gtcgatctcg agggcggtga agcggatgtc atttaggcgc agcatacgcc cgatgatctg
     5041 gccgtaacgg ccgaagcctg ctattaacac ggggttatgc tccgaaggca tatcgtcata
     5101 tttccattct gaagcggcga accgcaccct caggtagcgc gcattgagca agtagacgag
     5161 cggagttgcc agcaggctca gcgctaccac gacgttcgcg atggacaccg tttcgggtgg
     5221 caacagctgc ttgtctgacg agagtttgaa aagtacaaac gcgaactccc cgccctggca
     5281 gatgagaaag gcgatgttac gcgagacctc tgcgtcgtac cgaaagagat tgcctgccga
     5341 ccagatggca aatatcttga gggccagaag tgccagcacc gctagtggca ccaggagcgg
     5401 gtaacggagc agtacaccaa agtccatact cattccgacc gcaatgaaaa agagccctag
     5461 caataaacct ttgaagggtt ctatgtttgc ttccagctcg tgccggtatt ccgattctga
     5521 gaggacgacg cccgccacga atgtgcccag tgccatcgag aagcctgccg cttccataag
     5581 tgctgcgatc gaaaacacca gcaatagcga ggcggcagtg aacagttcac ggctctttgc
     5641 ccggactacg acacggaaga acgggcgaac ggcgaagcgg gcgcccgtga gcaccgccgc
     5701 gagtgccgca agaccgggca gcaggctcat gaagcctgaa ttcccctgcc ccgatgactg
     5761 tgtcagtatc ggcagcatga tcatgacggg cacaacggcg atatcctgaa acaataggac
     5821 tgcaaaagag gcccggccat actgcgtgtt gatctcgtgt ttctccgtga gtatctgcag
     5881 cgcgaatgcg gtagacgaga gggaaaaacc gaatgcgaga atgccgatct gcgatgtgga
     5941 taatcccagc attgccagtg gaacacccag tgcaaccata gaaagcacaa tctgtaaagt
     6001 gccaaagccg aatatatccc ggcgcatgtt ccaaagccgg cccgggcgca gctcaagacc
     6061 aatgataaag agcagtagca caataccaaa ctcagcaaat gccaggatgt cgctgacatt
     6121 gctgatcagc tttagacccc aggggccaat gacagccccg gcaatgagat atcccagcac
     6181 attacccagc cccagccgcg tcgcgagtgg aacggcaacc acggcagcag ccatgaagac
     6241 cagcatgttc atgagtatgc tatgtgaatg catcgatacc taccccggta ttatggcgac
     6301 agtctggcga atgcgcccga tcaccgcaaa atgattcgga caagataatc aaaatttcag
     6361 cgggagaaga ttttttctat tagcgtgcgc gtgagctttc agaagattat tgcatcgccc
     6421 tgtaccttcg ccaccagata accataccgg tgcagaccag cactaccagc gccaaccccg
     6481 gaatcagcgt gagctccttc tttgtaccaa agaactgcag ctggtgcagc ttcatcaccc
     6541 agaaatggaa cgcgcgcgca tcgtcgctct cttcgataat cgcggccgag ttgcggtcga
     6601 taataatctc cgggttgccc ggcgcgtcga agcgcacgcg atacaccgac gcgacaatct
     6661 taccactgcg gtggcggtag tgatcgataa tttcagcatc ctttatagta ggatttgcca
     6721 ggacatactc agcagctacc gccgctgcca tttgtttgtc aagcgggctg agcttctctg
     6781 ccgaaatggc gtcgaccagc tgtgaatgtg ccgctttacc ctcgcgccgt accacctcat
     6841 agacaagcct gccgccctcg gcgcgcaata ccaccgattc ggccgggttt accttgccga
     6901 cggtctctgt gaggcgtatt gcgtcttgcg gcgagagccg ggcgcctgat agagccggcg
     6961 ccccctgcac gtgtttcttc tttagatagc cgtctttgaa atagaggtgc ggtgcatatg
     7021 cccagagaat gccggtgatt gcagtgacgg cgagaaaaat tgccgacagg agcgcaaccc
     7081 tcctgtgcca gatattaact ttcatcgcaa tacgtctcag attctttctc cccctctcca
     7141 tatcatggag agggggccgg ggggtgaggt aattcttttt tgaaagtata cgtcgcgcga
     7201 tgacgcaccg attcgtaatc cttgccctca aacttaccgg gagtcttgtc ggtatggatg
     7261 acctcaagca cgtactgacc ggcccagggt gtttcgagct ttaccttacc ctcggcgtcg
     7321 gtcttgtact cctttgccca ggtattgggc gcgtagacca tcaacttttc gcctttcagc
     7381 ggtttaccat tgaagaacac ctggaaaaca ttctggccgc cggcaacggg cacgatgtcc
     7441 agggtctgcg ccggcctgac ttcagcgtta gcagactgcg ccccgtgccg cgcatagtac
     7501 attggcttga ccatacccca gttatacttc ttcatgtcgg cgacaggcac gtcgagactg
     7561 tcggctagta caaattcagc cttgcctgct tctgcaacaa agaactccgc cgtcttggta
     7621 ttcttcacct ctttattctg gcctttcttg tcggctgtgt agacgcgcag acccgttatc
     7681 ttgtcgagtt tgccgggtga cgtctcccgt tcgccatcct gaatttcgcc gtagtagacg
     7741 cgggcagtac cgccttctcg ttcaatccag atgtagtgtg ccgaaagaat tcctggcagc
     7801 agcagagctg ctgccagtag agttaacaat cgtttcattt ttaaatcctc gcttttgcag
     7861 aaatcgtgaa gttgaaaggt tcgcccggca gtgcgcctga aaaagtcggc gttgcgaaat
     7921 aaacttcgtt ggtgatgttg cgaaagttcg cctgcagctg ccagttctgc tgtttataga
     7981 agagcaccgc atcggccacg acataaccgg gcacgcgcaa aagattcagc gcgtcgacat
     8041 agagccccga cttcaccgtc ggcccgccgc cgaaacctaa gcccttcaga atacccgact
     8101 gcagttcgta cgtgatccag aaattgccgg tgtattcggg aacgtactgc ggagccttgc
     8161 cttcagtatt cacgcccgac acgcgctcgg tcgatttgat tttcgcatcg tagaatgcgc
     8221 tgctaaaggt aagccaaagc cccttaaggg gattgcccat gatatccagt tcgataccgt
     8281 cggtgagcct tttgccgacc gggctcacga cgcctgcagc atcagtcaca taataattct
     8341 cgcgcgtcgt gcgaaagaat gccaggttgg tctgcagttt gtctttgaac aaattcagct
     8401 tcttgccgag ttctatttgc gatgagcgtt cgggttcgac cgaaattgaa gtcgattcag
     8461 tctgtatggc catgaaacgc ccttcggaat aaccgccata gaacgagacc ggtttccacg
     8521 gctgcagtac cgcaccggtc tgccagctta tgagatcgcg cgccatctgg atagtccgct
     8581 gcgtcttggc cgcaccctgc cagccgtcgt ctttgatctc agcgtggtcg aggcgaatgc
     8641 ccgcgcgcaa cttcaggtaa tcggtcacgg ccacctgctc ctgtgcatat gcgctcacgg
     8701 tctggttcgt gatctggcga tcgaacgaag gtatcgccgc gtaatcatac gcgctgcgtt
     8761 cgagcacaac ggggtttacc aggtcagtaa ttgccggcag gttataatcg gtgcgccagg
     8821 tattcacttt gtatgactgc gcctcgatac cggcgagcag cgtgtgcgcc atttcaccgg
     8881 taagaaattt gagcacggct tcggtctggt agacaatgtc agaagactta tcgccctgct
     8941 cgcgcgcggt gcgcccggtc atctgcgtgc ccgcggcatt caccgcacca ccggcattgc
     9001 gcatcaggta cagatcgcgg ttatcgtaga tcagcgccga gcgcagtttg aacttcggtg
     9061 agaacacacc ttcgtgtgaa atcacggcgc ggtgcacctg ctgcgtcgca gtattgaacg
     9121 gcgaatagta ggtattcgat ttactcacgc tcatcagtga acgatctgcc ccaaaaagta
     9181 tgccgtagtt atcgggccgc gccttgatgt tgcggtagtc atagtcgacg gtaatcgaat
     9241 gcttgtcgtt tattttccag gtgaacgaag gcaagaattc gccgaccgta cgtttgagtc
     9301 cccgatagcc atcggtatgg tagtacgagg cattgagccg cgcgggcagc gcctgcgtgc
     9361 ccagcgggcc accgacatcc aggacaccgc ggtaagtgtt ccagaagccg cccatggttt
     9421 cgagcgaggc gccaaaagta tccatcggct ttttcgtcgt gatgttgacc accccccccg
     9481 gctcggcccg gccatagatt gccgagcccg gccccttcag cacctcgacg ctctcgacgt
     9541 cggtgttcgt gcgcatatag ccgttccagg tattgccgtc gggcaaaccg tcgcgcatga
     9601 agcgcatgtt gagtccccgc agcataaaac tgtcagagaa accataccca cccgcgagcg
     9661 tggtcgtaag cccgctgacg tttcgtgcca tctggttcat gtcgaccgac tgctggtctt
     9721 caatgatctc tttcggcacg acaacaatcg atgcgggcgt gtcatgcacc ggtacatcgg
     9781 tcttcgaacc ggtgacacgg ctattcgcct gcaccaccac ttcgtcgagc tgcgcttctg
     9841 caggtactgc aggtgctgcg gttgtcgcct gccgcggatt aactgctgtg ggcgctggct
     9901 gcttcttatt ctgaggtttt tgcacaggtt gtgcaaagac tgctgcagcc gaaacggcag
     9961 ccactccata tactatattg attctcattc tcagttactc cttttattgc ctcacccccc
    10021 ggcgcctggg cgcaagccca aggtgggtga ggcttatctc aactcaaatc taattggtac
    10081 tctgacgcgg catgcgaccg cattgccgtc tttgtcatag gccggtgaaa agacagcgtc
    10141 cctcaccttt ctcagcgcga cctcattcaa cgcatcgtag ctaccctcga gcagctgtgc
    10201 atcgcgtacg cggccttcgc tgtcgatgat cacgctgagc acgacgcggc cgtctttacc
    10261 ctgctgcgcc gccagcagcg ggtaatcatt agctgtgata aagttgccca cccagcgcgg
    10321 tttgcggtgc gcgtctgttg cctgaatcgc atcgccgaag cccgttgtct cgttgcactt
    10381 gccctggcat acgctggctt cacgcggagt ttctttggtc tgctcggcag gcttcagctg
    10441 ccggcggttc ttctgcacca aaaaccagtc gtcgttaacc tgctccggta ctgcgctgtt
    10501 cgcggcccgc ggcaccagcg gtgctgcaga gagttcgaca tcgtgcaggt gcatatttcg
    10561 gccaaaggtc acccaaaggt agaacgatcc ccctgctgat gcgtgtagtg caaccgatgc
    10621 cagaagagag gctgaggtga atttactgag gctcttggtt aagcttggca tatgttattt
    10681 ttccgaagac agcgcgacgc gggtcacgcc tgcctgtttg atgaggtcga gaattttcac
    10741 gacgtctttg tagataagcg cttcgtctgc ggcgacgacc acacgcacgc cggggttgag
    10801 ctgcgcctcg cgcgacatgt tggctttgag cccattcagg tcgaccttct gccccatcag
    10861 ttgcagctct ccgtcttttg cgaccgtcag cgtcagcgcc ggcgttgggt tcacctcggc
    10921 cgcggcggcc ttgggcaggt ttactttgac tccggtgtcg gtgacaaaat gcgcagtcac
    10981 catgaagatg atgagcagca ccaaagtaat gtcgacgagc ggcgtgacat tgatgccggt
    11041 gacgacgccg tcttcttcac tatcgaggtg caagatttgc ctctgtgtta cggattgccg
    11101 aactcacgaa atccccctga ggttgtgcgc gcaggtagac ctgcatcagg tgaatgagac
    11161 tctgcgtgtt cgccgtggcg cgtttgagcc gtgtctggaa ataattattg gcaatcaccg
    11221 caggtatcgc gagcaaaatg ccgaaggctg tcgcaatcag cgctgcggag atacccgtca
    11281 tcaccaccga aacacccgcg ctgccggcag cgccgagatc gttgaatgcc ttgatgacgc
    11341 cgagcaccgt gcccaaaagc ccgatgaacg gggcgttgtt acccatggtc gaaagcacga
    11401 tcaggttctt ttcaaaattg ccgcgctcaa tcaccagcgc cgacttcatc gcctcttcga
    11461 ccgaatgtgc gcctttggta aaattcctga ggccctcggc cgcgatacgg ttttccatag
    11521 ctgcgaactg cgtgcagtac gccagggcat tgccggtctt gccctgctct agcagttctc
    11581 tgaggccgct aaagaaaccg gcaaaatcga tgcggttctg ccgaaagagc cgccagcggt
    11641 cgaacatgac ggcgaccgaa agtatgctcg ccgccaaaag cagccagagc acccagacat
    11701 cgcccaagag ggcggctttt ttgaagattt cgttcatggc aatttcctga ggacaccttt
    11761 cagaatacac ttatattgag aatcattatc aatatcttaa ccaaagattc tccgccctgc
    11821 ctccgcattc accgtggttc aagaatccac cacaggctgc ttagaaagca gcctgtggtg
    11881 gatttatttc tgcatgctat cgaaatctga ctgcatcagg gcttgaacac gcgctgccaa
    11941 agttggggcg tctttgggcg taagccccgc ggtcgctacg ggtgccagca cgtgtacgcg
    12001 cagcatgccg ccatatcttt tcgctgccgt atcgataacc ttctttaatg actcaatcac
    12061 gaccggaacc acagggacac cggcctcaat cgccatctgg aaggcacccc gcttaaaagg
    12121 aagaaatccc acctctttac tacgcgtgcc ttcgggaaag attgcaatcg acacccgccg
    12181 gtttttgatc gcagcgacac cggcgtcgag ctgccccctc gccttcttgc cgtctgcgcg
    12241 gtcgatcagc acgttaccag ccaaccagaa cgcccagcca ataaacggaa taaaggcgac
    12301 ttcctttttg ccgatgatcg ccagccgtgg aatgcgcagc atacccaacg gaataccatc
    12361 cagcgaactc tggtggttta aaacatagat agcgggctcg cggagtactt tttcagcacc
    12421 ctggagttga atgcgaatac cgctcaggcg ctgcgcgacc acggcataaa gatacataaa
    12481 aaggccggtg ttaaccgggc gacgaaatgt aatgacgagc cagggtaacg ccagggcgca
    12541 gagcagcatc agccagaact gtgtgagata catgcgccac atgggtgtgc ctctttcggt
    12601 tcagctggcg cgtcaagagt acttgtgact tgcctgacct tccacagcca gccagcgctt
    12661 tttttgcttt tcataacgcc gggtgaatgt gcttacgcct gtggcgcagg cacacgaaat
    12721 tctcgatgtc cgcaaaaacg gagctttcat cttcagcgac attctgctga tcgattaccg
    12781 gcagcagaag ctcggaagcc tgctctctga aatcaaaatg cagtcccacc agctcgaccg
    12841 tatgctgaaa aaatatacgc atgatgatga gcgattgctc ggccaggaaa aagccaacgt
    12901 cgtcaatgta ctgcacatga ttatccgcaa cctggcagcc ctctaccttc ggctttttga
    12961 ccttgaacac tcagcagagt accacagcga aatcgaggta tcagagggca aagacggatt
    13021 tcttatcaag ggcaagatgg atgcagatga agttaatatc cgcttttcgc tgaagcagtg
    13081 gacagacgaa tacctcgacg ctgcacttgc cgacctgttc aaagacttcc aggatgcggc
    13141 aaaggaccac gttattacgc tggctgaaaa agtcgaactc acaggacgta tcgcgcgcat
    13201 cctggtaaaa accatgcagg ccttttacct catgcgctct ggcgcagtct ttcgctgatt
    13261 attcagcagt gctatactga ccgacaaact cgatgaagcg catcacctcg ggcatatctt
    13321 tacccgtgaa acgaatcgtc gaaccatcca cacgttcaac taccggcagc agtgagagca
    13381 cctctgcgtt catcatgttc ttgaaagcga tctccagctt tactggtttg ggggtggcgt
    13441 aagctttgta gcggctgagg tttgccaaag cttctttggt cccgttctgg atcgcaagcc
    13501 gtgcctgctg cggcgacagg ttatcggcgg agtgataacc gattgcccgc ttgaccacta
    13561 ccccggtaat tttttcgctg atggttctgc gtgcctcttc aatggtcttg tcatcacccg
    13621 agatgagtac gacaggcacg ccatagtggc ctgcaaccgc ggcggataac aaggcctcgc
    13681 tggtatgcac gccgttcagc cgcatgccgt agatgcgctt cgacgacatc gtatgcgcgc
    13741 ggacggcacc ggccgtgcca atggatgcgt ggtatcccag cagaatgacc gcatcgtaac
    13801 cctggtcgat accctcaacc atcgccagcg gccggggcca ggagcgcaca aggcgtgcac
    13861 gcgggttaag ctcttcgggc aaaagcgaaa gcccgttgcc gtgagagtct gcgaccgtga
    13921 catgagtcgc tccggccgca aaggcaccct caatgcaggc attcacctcg gcagtcatca
    13981 gtcggcgaaa tttctcgtat tcaaactcgc ccggtgccag ctgcgccccg ttgacaacgc
    14041 cggcaacacc ttccatgtcg gcagatatga acacacgcaa cccctttcgg gttgagtcat
    14101 tgctgccggc gacagaggtc accaccagga aagaaacagc gcaccccaca ataagctccc
    14161 ggacaaatcg gctctgcatg gctggcgtca tgggtaacag agcccaagcg tcaaggtaca
    14221 caggaccagg cacctggcag aagacggcca aatgtcgttg acgtggtgtc tttaaggccc
    14281 atcttatcag tagagcagtg gcatttgcta ctgctttttt tatgccctat gagttgcctt
    14341 caccgcctgt gtgcgggccg gcgaagcagg caacaaatac attttttgtg gagaacagat
    14401 gagcgaaaca gagacagtta ccgaaaccca gaccagcggt gccgaatcac tcgaagcccg
    14461 tatacagagc cagttcaacg aggaaaagtg gaccaggctt tcggcaaaag acgtaagtat
    14521 ctctcgtttc aagattctcg aacagatttt tgatgaagta aagaagcaaa acaagcagga
    14581 tctcctgcgc agcgagtcac aggcacacct ggccgagtat gaccagtcca tagccgcgca
    14641 ctactttctc ggcatgatgg cgctcgagaa gaatattcct gatgaaattg ttcacctgaa
    14701 aaccctgctc gaccagttcc aggaagcgtc gaagtgggct gttgtcgatt atctttcaga
    14761 gaagatgctc gcggttacca agacgcgcac tatcctgcgt gcacgcgcca acgcactcga
    14821 aaaactcggc aaacacaaag aggcaattcc tgtcctagag caactggccg tacttgaccg
    14881 caagaaccct gacgttgcgc tcaaatacgc cgacgccatt atcggcgacg acatggagaa
    14941 ggcgctgcag ttctataagc aggcagccga ggcctttgca cgcacgctgc aatttgaaaa
    15001 actcaagaca acatggaacc ggctgatcga acttctgcca gacgacttca atttctataa
    15061 gaagattgag cgtatcctct cgggccatcg ccagcgcgaa atgatcgctg agctttatgt
    15121 tctgctcgcg aactatttca tcaagaaaga agaccatgac aaggtcatgg cgcttagcaa
    15181 aaagattctc gaatacaatc cgggttacgt gcgtttcaaa aacgaactga tacgcactta
    15241 ccgtgaaaaa tacaaatccc actcgctgct cgaagacttc atcaagtact cagggctgct
    15301 cgacgcgcgc aagagcttgc agtcttcgat cctgaacttc gaaacgaaca tcgtttttga
    15361 caaaggcaac tacgtttttc accgcagctg gggcgtgggc aagatcactg agctcaatac
    15421 cgccgaaatg gtaatcgact tcaaagataa aaagggtcac aagatggaca tccagatggc
    15481 cctgaaatcg ctcaagccat tgcgcgaaga acatttctgg gtgatgcagt ttgaaaagcc
    15541 tgaagagatg gcaaaactgt tcaaagaaga tattacgcag ttcttcaaga ttctactgca
    15601 gtctttcggc ggcaagctgt cactcgctga catcaagacc gagctgacag acaagtatgt
    15661 gccgctgtcg cagtggtcaa agtggtgggg caaagtacgt ccggaactga tgaaagatgc
    15721 gctgattggc atttcggcgc agaagaaaga catgatcgag ctgcatgaaa caccgatcac
    15781 cacgtcagaa tctgcgatcg aaaaattcca ggctgcgcag ggttttgaag atcgcgtgca
    15841 ggcttttatt gatgttctca agtcacctga aggcaacctg gaagcgattg aatttattgc
    15901 cccgcacttc cgcgaaacgc tgaagagtct cgatcccaat acccgcctga agagcctgtg
    15961 gctgcttgaa atggcaacgg aagcgcttgc tgacgacgaa cccctgttct cgaaagagca
    16021 gcaggcggaa attatccagg cgttcaagag ccggacaccg aatgatctga cagaactgct
    16081 gcaggaaatt cgccagagcg aactgaagcg ccacgcgctc aagttcgtga agcgccacca
    16141 taacgactgg aagcgccttt atctggagat gatgtttgaa acaccggtga agctccacaa
    16201 gagtatcctt tccgacctgt cagcggccaa cgccacagat gagataacag aatttgttaa
    16261 ccggttgaaa aaagatatca aagaaaacgc cgaagtcttc ctttggacta ccaagtcagt
    16321 gattgccggg cagattgaac tgccggcagg ccagtcgcag gaactgctgc tcggtctctt
    16381 ccgcatgctg aagtctattc ccaaaatcga acccaagggt tcaaaactcc gcaatagcgt
    16441 gcgcgatctg ttcttcagca atggtgcctc agagctgatg gcagtgatct cacgcgaggc
    16501 aaaagactcg gtccgccgct ttgcatcgct gctgaaggac gtaccgttct tcaacgacgg
    16561 cgagaagatc cagattgtct cgcagctgcg cgaaattaat cctgcagtat ttgaagaaca
    16621 acaggacgag agcgacctgc cagaacagac agaaagcctc gcgcaaaaac tggaaaaaag
    16681 tggcgccacc gtggcgagtg cagacgcgat tgaggcgatg aaagcccgcc tcgaccagat
    16741 tatcaacgtc gatatgccga agaactcgga agagattggt ctcgcgcagg aaaaaggcga
    16801 cttgcgtgaa aactccgaat acaaagctgc gctcgaaaac cagacaattc tgcaggcaac
    16861 ggtcacgcag ctcgaagccg atatcaaatc agttcagcag ctgagcgccg aagatgtgga
    16921 tacaagtgtc gtgtcgattg gcacccgcgt caaactgcgc gattccgcga gcggcgatat
    16981 tttcgtctac tcgattctcg accagtggga tgcggacatg gataagggta tcatctctta
    17041 caagtcgccg cttggcaaag ttctgatcgg cgcacgcaag ggcgcttcgg caaccttcaa
    17101 tgcgcagaag ctggaagtaa ttggcatcga gaaagcaatc gacaatgctg gccggctgat
    17161 ctagccggca ggagggtgag aaaatgaacg cgctttcacg tctggttttc gcgctcgttt
    17221 tgaccgccgg tgccctgtca gcccagggta aaacgggtat cggcggcacc gcttttgaaa
    17281 tcatggatcg ggtgttccaa cagctgcaat atgcagaagg gcaccagaag ctgcaactga
    17341 ccattaccac tcgcgaaggc aagtctgtcg tctacaaggg gtcactctac cagaagcaaa
    17401 atgccagctt cttcgtgttt gattcgatga cgcgcggtac agtgctgaaa ctgctctata
    17461 acgaccaggg gcagaacatc tacgcgtacc agacgcacga caagcgcctg tttcacaaga
    17521 agtacctcga ccgttttgaa agcgttctgg gctctggctt tgcctacctt gacattgcaa
    17581 atgcttcgtt cattgacaac taccagcaga aaaccaaggg taccgagaaa aacgacgaag
    17641 gtgaattcac cgttatagaa aatatcccgc tggacaaagg gcactacggc aaactcatcg
    17701 tgctcgttga cccgaaaaaa gacaatcgcg tgaagcggat tgattattat gactcggcca
    17761 tggtgctcat gaaaaccatg acattcacct acggcatgct ctcagtgaaa gaagaaaaaa
    17821 acctgacccg cgaaattgaa tttcctattc gctgggatat ggcagacgtt agccgcggta
    17881 cggtcagtac gctcgagttc ttctcggttg acaaaactgc aaggctcgat gcgtcccttt
    17941 tcaagaaaga gaatattgaa aagtaacccg tgagcgttgc acctacccca ctcaggtcgc
    18001 tgcagcggga acacgcactg ctcttgctgg cacaggaaga atttacccac ctggtcgaac
    18061 gcggcaagct gccgctgcca gcaaggcacc tggaacattt ttcgagggtc atgcggcgcc
    18121 accacagatg gcgagctttg gccggcaacg gcatgggcac gctggccgtt tgtgaagtgc
    18181 agaaagatac tgttgtactc acagctgaag gtatacagga atttccgcgg acttcacccc
    18241 gcgtaaccct tgtacaagcc tggcccaaac ccaaggcgct ctcgcttatc ctgcaaaaag
    18301 ccgccgaact cggtgcgagc gagctcgtgc tggtgacgtc agatcatgct gcgcaccccg
    18361 cggagaaagc cgaaagaatg gaggcaatcc tggaaaacgc ctgtatgcag gcatacaacc
    18421 tctataaacc atcggtatct ttgagggcgc tggcagacca tgcaaacttc ctggatacca
    18481 tggcgttttt cggggatatc cagggttcag aaaaaatcag gaacctggca atgccagccg
    18541 gccaaaaggc gacatttgtc aacggccccg aggggggatt ttcgacccgc gagacagatt
    18601 ggctcaggga acgcgcgacc ggcgtcctgc tttctgaaaa tgttttgcgc tctgaaacgg
    18661 ccgcaattat tgccttaggc cacttttgcc agagtccatg actgtgaacg gagaaaaatt
    18721 tgatcacgcc tcgctggcgc cggcatacga tatacagtcc ttgctgcgct gcctttcgct
    18781 ttcggagcgg cgcgtggcca tcgaagtaaa tggcgaactt ttgagcaact cccaatacgc
    18841 cggcaagcag ttggcggaaa acgacaacat tgaaattatc cactacgttg ggggcggctg
    18901 acac
//
LOCUS       DAOQJF010000012        18507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1394_length_18507_cov_4.569423, whole genome shotgun sequence.
ACCESSION   DAOQJF010000012 DAOQJF010000000
VERSION     DAOQJF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 18507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18507
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1394_length_18507_cov_4.569423"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLF85_01320"
     CDS             complement(<1..675)
                     /locus_tag="PLF85_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE18322.1"
                     /translation="MEINLKEVKESFVTTTRDLLQRSEALLLELERGHNPEHFTEMLR
                     AIHTIKGNAGIFELEGVIRLCHALETFLEAFQSGNLALSAGQIDLGLTVLDRMRSQVA
                     VMDDPQKLAGIPAEDLLAQLAGKEPGTVQVPSSAAEEGRDSFDRLREHTQKLRLPEKY
                     LEVAKSKNRYLIFLVVDLGDQGQTTISQLYAQLLSLHKSGNLLSLGVLRKETITSNPA
                     GPFLPYF"
     gene            complement(675..983)
                     /locus_tag="PLF85_01325"
     CDS             complement(675..983)
                     /locus_tag="PLF85_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18323.1"
                     /translation="MNFFRKKTTAFITLEAPDFLAETSKLWGDLLFALEGHPVNQVTL
                     RLYDSGDLSLRTLSVVVSLGLKLRTQNVAMELEVSPRLAQMLRRLNMSSAFTRLTEVN
                     "
     gene            1306..2376
                     /locus_tag="PLF85_01330"
     CDS             1306..2376
                     /locus_tag="PLF85_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE18324.1"
                     /translation="MPDSLYQSIPVLLVDDDRAVRRSMRRLLESWGCTVDEAGDGHAA
                     LEKLCVPGRHLVMTDIDMPSLDGFDLLDTINNRFPHCGVIMVSGMSHEKIIDAALERG
                     AIGYIQKPFRLPELKAQVTNALRRMSQDQGIESADRDAAREFARMFAATADLHHVETG
                     DHIRRIGRLSRELALRAGLPHTSAVLLGEAAILHDIGKLAIPDAILKKAGALTPDEFA
                     IMRTHPVLGAQILRESRNPMLQLAHEVCLHHHERWDGMGYPHGLRGEDCSIEARIVGI
                     VDVYDALTEKRVYKEAWPLDKVISFFEERAGTAFDPRLVELLLADIDNFEAVRLAADD
                     VSDVYLRPGLQVKVQTGAAIAV"
     gene            2423..2722
                     /locus_tag="PLF85_01335"
     CDS             2423..2722
                     /locus_tag="PLF85_01335"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE18325.1"
                     /translation="MTADRREGERLSVLRRIIIIAGKKRYDGYTIDISRGGISFIAAG
                     EIVCGPAELQIPELRSFLKGNIVDMEESDPRRLRRYHMTFLSPMESSEIELITAI"
     gene            2802..3326
                     /gene="lspA"
                     /locus_tag="PLF85_01340"
     CDS             2802..3326
                     /gene="lspA"
                     /locus_tag="PLF85_01340"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNE18326.1"
                     /translation="MSRWRTKFTPLFLTVLGVVYISDLLSKLWILKKLGGIGQSETII
                     SNFLYFTLTRNEGGVFGMMQGHAWLFQILTGFAIIFLLYYYYKTPETSLIFSLAIAAI
                     LGGALGNFTDRFYGTTPGVVDFIDIRLGSFRWFIFNIADAFICVGAFLLAIAFYQFEK
                     AEKAAAAAAQQEKA"
     gene            3328..4599
                     /locus_tag="PLF85_01345"
     CDS             3328..4599
                     /locus_tag="PLF85_01345"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNE18327.1"
                     /translation="MPATLIVGTQWGDEGKAKVIDYLAKETDIVVRYQGGANAGHTVK
                     VGEDKYIFRLIPSGILYPKTICVLGGGMVIDPDAMFDEIAELEAKGIQPTGRLRIADN
                     AHLLMPYHKEIDVRSEEASGAHKIGTTKRGIGACYSDKVNRQGIRIGMLYSDEFFAST
                     LPRLVEQKNRLLTKMYDAPPIDLAAMKEKLLALREKLRDMVVNAPYYLNMELASGKRV
                     LLEGAQGTMLDLDFGTYPYVTSSNPTTGGALSGSGVSFQYLKSVIGIAKAYSTRVGEG
                     PYPTECFGEEGEILRKAGNEFGSVTGRPRRCGWFDAEVVRHAARINGLTSIALTKIDV
                     LDTFKTIKVATGYELDGKRIDYFPSCNVANVKVIYEEFPGWNEPTSHITKLKNLPKNA
                     RRYIDAIAKFCGVPIAWVSVGPGRENTIAIK"
     gene            4654..5223
                     /locus_tag="PLF85_01350"
     CDS             4654..5223
                     /locus_tag="PLF85_01350"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNE18328.1"
                     /translation="MTVKELFTYRILTISNLLSLSRILGIPAMWYILAHPHDISSIRL
                     YTALTLFFMTLTDFLDGYLARRLGQETPLGQYLDPIADKIVIISGLGLLVYYRDFPLW
                     VTIFVGLREIIGTVGGGYLLIKRNVLGKPNYWGKTGVAMVSLSGLFYLFNWPYREWTI
                     PPLIAVFAGGILAYAKTYGRTIFKGSEHG"
     gene            5186..5971
                     /locus_tag="PLF85_01355"
     CDS             5186..5971
                     /locus_tag="PLF85_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18329.1"
                     /translation="MDARYLKGVNTVNLYIYLAAAVPLQLGFAAIYFAVMHFSPQNVL
                     LREALPVAYLAVTYLQWLTFSFWISLRSKKYRVPIVMHLPHLLSFMPLIRHIMLAADR
                     QAVDATSLNLAWIHSVVTHSFSLLAAWLWQVRAEFDDGTGLRHRILPIALYILAALTT
                     LVVYAGLDLSFARTTLPFGLGLVQLLLYTSVTLLLLLREKIRHPLPAIAAAAFAIVNA
                     ILLFDQARSDIGVEFALAAGSATQLFSLYVFLVFSAHRPLAKR"
     gene            complement(5961..7298)
                     /locus_tag="PLF85_01360"
     CDS             complement(5961..7298)
                     /locus_tag="PLF85_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE18330.1"
                     /translation="MTAVTATDTPGMQKLHRLKMLLWQSLTRRGLLGIILSCALISFY
                     VLLYWSGEIDQLWAASHVNQVLAPLERKISAARGIAAEQGLKDQKSYILASYREQAAF
                     GGGYHRYLLPEYRVFVDRLRNTSPAPVTSWVDRTSAVLNPVSHFLRNRDSDRWFLYGA
                     LYTLLVVSMGIRFYRRWRHDRYQKIRTLSVMAVQSVIAFMLPALLLYFNTKEFYFSYF
                     WPLKLEYFLPSTFTDYPRAMALWAVMMSFVAVPVLTYFFGKRWYCSWVCGCGALANTM
                     GDPFRQLSDKSERAWMIERITVYSILAITVVLTGLIFINEFRGINDGFAQWVFSMKRW
                     YGFFISAGFAGVIGTGFYPLMGTRVWCRFGCPQAAILGILQVFFSRFRISTNGGQCIA
                     CGSCSANCEMGIDVRSYAMRGEDIKRAACVGCGLCMSVCPRGVLKLGNKPLSV"
     gene            complement(7373..8719)
                     /locus_tag="PLF85_01365"
     CDS             complement(7373..8719)
                     /locus_tag="PLF85_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE18331.1"
                     /translation="MTVQAVLPPGPKVNPLSLIADFATGGPLLVTNPLKYFEDLHREY
                     GNLVFSDLGIGRFYITNNLALIRKVFVSEPRSYAKADAYKEAAHFLGNGILNSEGDFW
                     QSQRQMVQPAFTRSRLGSFVSTMVDVSDRAIAVPGSVADVSALAMHLALENICRFLFG
                     EDFSANERTIREAVHFGNSFISSRIQAPLKLPVYFPSLNNLRFLWHRFELDQILYRII
                     DERAQRSDAGSDLLGMLLKAERERNQGKIRRTQVRDEILTLLIAGHETTGFTLAMALW
                     LIARHPQVQENLRDEISAHGAGESVDGLEKSQLLEAVVNETLRLYPAAWIIGRRIVAD
                     EEFEGYLLRKDSQLQTGIIALHRNPQYWSAPASFRPERFLGETPEPVQKQAFMPFGAG
                     PRRCIGSMFAMLEMQVAIARILRRYRISAPDNNEPQINYLFTSTLKNPLPLHFEPL"
     gene            complement(8769..9620)
                     /gene="lpxC"
                     /locus_tag="PLF85_01370"
     CDS             complement(8769..9620)
                     /gene="lpxC"
                     /locus_tag="PLF85_01370"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNE18332.1"
                     /translation="MPQIYRTTIKHPGTYEGFGLHSGEVTKLTFYPADCGDGIVFFRE
                     GKNGTERTAITATLDNVIDTSLAVTLGSGENSFQLQTVEHLLYALFVLGITDIAIEVE
                     GGKEVPIADGSAGPFIRSLQQLELHQYTERVKPLVVTRPVSVTDGERYLVAMPAREFQ
                     VSYYIDYPHPLLKNQAVQLGYEDLVFTETIGFARTFGFMKDVEYLRSRGLAQGGSVDN
                     AVIYMPDGTTLNDFRFERESVYHKILDLIGDLALVGRPIQGHILGARGGHALDVAFGK
                     KLLAAFG"
     gene            complement(9629..10588)
                     /locus_tag="PLF85_01375"
     CDS             complement(9629..10588)
                     /locus_tag="PLF85_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE18333.1"
                     /translation="MATGTKAIYYALAANAGIALTKTVAAVITASTSMTAEAIHSFAD
                     CGNQVLLLFGMKRAKAPATELHPLGYGKVSYFWSFLVALLLFSVGGLFSVYEGSHKLL
                     KPEPLQYPWLAIAVLAIGVALESFSLYGALKEVRASRGHRSLYRWFRETRQSELMVVI
                     GEDIAALMGLVVALLFVSLALVTGNPLFDAVGSIVIGILLIAIALMITLEIKSLIIGE
                     SADEAFKGQLRSFFSEKYPHAQVLNLITQHHGPDIVLALKVAFRRWPVSARALVDQIN
                     AIEKSIRQQFPAVRFIFFEPDISSSEKQKRVAARRKTKVRARK"
     gene            10661..11344
                     /locus_tag="PLF85_01380"
     CDS             10661..11344
                     /locus_tag="PLF85_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE18334.1"
                     /translation="MRNLPRLLITITLMQCFFACRRAENTEHPRGAAEAERLAAILES
                     PDRAHWQMPDRVISELRLKNGEVVADIGAGTGYFARRIAEKIAPAGLSVGFDINPDLV
                     AFMERDAATRGLSANYKARLIASRNPTLEDGHYDLVFFCNTYQHLENRTAYFNSLAKS
                     LKPNGRIVVIDFIKLEKTDEESGEVPENLVDKETVKREFRQAGFKLRRELKFLPSQYF
                     LEFVRRPVG"
     gene            11349..11615
                     /locus_tag="PLF85_01385"
     CDS             11349..11615
                     /locus_tag="PLF85_01385"
                     /inference="COORDINATES: protein motif:HMM:NF039366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6171 family protein"
                     /protein_id="HNE18335.1"
                     /translation="MLGFFTKLLKAQPNAGCSDCAKREKYTLLNAAADTLRGDFASDQ
                     LRDSRLEICKACEYLAMGSNCKLCGCFVHLKARYAAASCDINKW"
     gene            complement(11619..12293)
                     /locus_tag="PLF85_01390"
     CDS             complement(11619..12293)
                     /locus_tag="PLF85_01390"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNE18336.1"
                     /translation="MRRLVCLAETDSTNNWLKRQSALRAFTSVRAVLQSAGRGRAERS
                     WFSGSPGDNLAFSILLPYDASLAGLLPAHVALVCHEVLSHWISTRIKWPNDILFEGRK
                     LAGILCESFPAEQNLFIVGIGMNVAQRDFPPDLRDMATSLAQATGQLHDAGSLWLRLT
                     RALWREFRRPRTAQEIIPAYDRVAVRYHRRREYPGEILEFETLLSDGRAAFLLGGERL
                     MLDQAS"
     gene            complement(12302..12637)
                     /locus_tag="PLF85_01395"
     CDS             complement(12302..12637)
                     /locus_tag="PLF85_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18337.1"
                     /translation="MGALTITLLALSGATFIYLAWLSLQLHRQKPAELSWRGIIKLVS
                     LAGVPAAISGIYARWFRAFWAFFLLGSLAVACAVWGTDHSFLVVIFPLLFYYFFARYF
                     LWEKEDLTD"
     gene            12764..13384
                     /locus_tag="PLF85_01400"
     CDS             12764..13384
                     /locus_tag="PLF85_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE18338.1"
                     /translation="MHNFNTYLAQGIGSLPSSLVSAVLILISILALTVALERGYILFR
                     ANVPINPDEARQLIAMIAAKKYDEARDFCSGKLHPGYRAVQRIIEGRSLHPDLIGFAR
                     EAILHEQTMLERFTSFLGTTTTLAPMLGLLGTVTGMIKAFSAFAQAAQRSQQLTIGIH
                     EALITTTLGLMIAIPSLVFFNYYVKRTNQLMDECELLVDRAMKELA"
     gene            13386..13787
                     /locus_tag="PLF85_01405"
     CDS             13386..13787
                     /locus_tag="PLF85_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNE18339.1"
                     /translation="MLTRSRQRLQLRTGIELAPFVDILFLLVTYFLMNATLANNPAIR
                     IELPKSDTSQAAESQPLVVLINQDNQIFVNDRNVPLKDLGKTVADMVKDKEKDQVIIR
                     GDKRSSYQVVISVMDELNKAGVTRFNLATDK"
     gene            13791..15863
                     /locus_tag="PLF85_01410"
     CDS             13791..15863
                     /locus_tag="PLF85_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18340.1"
                     /translation="MAARPGRIDRAVLLFRGTAFLQGFAFIVYFRSLLDNLYPFYRGL
                     FPLSQFALLLFALGFVGAALYRPAGTTRVRRVLAGLLLGLPAAGSLIALYHFAGRERA
                     LLIFPLPVTAVSLFLWYELSRHTFVFQPAKIWLIFGALAAFFAGGLRDFPMVAPACAF
                     TAILLARLLSAPEWQVAHERIVTLRQLIDFLRYLFLAQAFHGVLGQNRSNLVAVVSLC
                     MAGLAIPQVIRLAGTRRPHIQSGLLSLPLVFMGLAALYNALHYNLWGALAYTLLAAWE
                     GVYFARAHEVYLRREKILAGIAIAAGLVAYYISTEWLQILAGSLVTLFLAGILIYVAK
                     NWRKAITALFAMAVMGWLLALQWKYTHSVTRDFYRPPVTKLRAAQLPDTGLVMTIMKL
                     QKNTGKTIRTNMLPDELLADAAWRTENISTFNADPATLVLRLAYDCDFRREAAVYIFD
                     EKSLGIYAESQALMVLREYFMRVPHCQMFLADGQALREVTNLSQVLQSDPGQAQNLAP
                     EDAAKILGLARVVKKRDLTAESQGLYEQVFRFYREDPVFLRELSTLAAARGQIDRQIE
                     ILNLLITLRKDNTMYDKKLLMELYAIRNERKKSAALAYEILADGSESPLAMFTFLQKL
                     FSEPFDRGEMQSLYQKIAQYQPRTDLEIIKYAGLKRSIEDRLKQNPTYDRKFMDENQR
                     QEFISFPE"
     gene            complement(15868..16662)
                     /locus_tag="PLF85_01415"
     CDS             complement(15868..16662)
                     /locus_tag="PLF85_01415"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNE18341.1"
                     /translation="MRLIWMQIALGIAIIIETAAMHRSALRQIFELSWATAIGKGYLW
                     QLVTASLVHTNPFQLLFNLLTLWMFGSELEQRWGRLAFLRYYLFCAVSSALVFLLLVA
                     LFPGMRYDVFSSATGANLGLLIAYAIYWPDRQVWFFFLFPVRMKFVALITGFIALSYA
                     VSAAPFVLSAAGHLGGLIAAGLLLLVTGGEPQRVGRALGSLGNFFSSRIRRPAADHGA
                     PVQPGDMEARIDVILDKISRDGMKSLTAAEKKFLRDASERMNRKKH"
     gene            complement(16697..>18507)
                     /locus_tag="PLF85_01420"
     CDS             complement(16697..>18507)
                     /locus_tag="PLF85_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNE18342.1"
                     /translation="LLTAETDITVMGANASRLFVGTSNRGIFESQDQGRTWLHHHAHR
                     IPMALDPANERRVYAVDNTLEFATHKHDLLFRTEDPQQRWQTVKTNLKRAVIFTALFR
                     GKENLYMGTSVNGLEVARLSEPGLKSAQKPGGKLVLKFASLNAGLPGKAHNKSHFIYE
                     EVQAIHETGEGHLLVASGPRPAVYLKEANAARFRKIDTGMLGEEMDECFSISSTGASN
                     IVLSCRRGIWQGPATGGGWIFTPQSKVVGDVADYAAYALLDTEGNRLSFTAPKQRLSA
                     EKQARMQNASGQRLLYSSAFTWQKKQPKVLGELTQNFWTGLVIDGKDDNGIIRYDSDV
                     PVARAIGAVRPLYKIRDVVEKVHALGKRVVVRLVIFKDPKLFEREGYAIRDAGGGKWI
                     GSPKERWIDPYNPNLLTEYYGPIVRELTERGVDEIQLDYIRFPSDGAIGRCRYSHKKS
                     DHYASEALENFLVGIRQQTHLPLGADIYGYNGMYRVPGAIGQDLEVYGRILDVISPMH
                     YSSHFGDDYMREYARDDRAYQLLLLALSRGSFYAQGNFILRPYIQAFPMKNGLWGYGK
                     KYFADQIKGSADGGGNGFMFWGQIDHMVLVRQTQSE"
BASE COUNT         4232 a         5255 c         5018 g         4002 t
ORIGIN      
        1 gaaatatggc agaaaaggtc ccgcggggtt ggaggtaata gtttctttgc gcagcacgcc
       61 gagcgataac aggttacccg acttgtgcag tgaaagcagc tgcgcataga gctgtgaaat
      121 agtcgtctgt ccctggtcgc caagatcgac gaccaggaaa atcaggtaac gatttttgct
      181 ttttgctact tccaggtatt tttcaggcag gcgtagcttt tgcgtgtgct cacgaaggcg
      241 atcgaagcta tcgcgcccct cttcagcagc cgatgagggt acctgaaccg tcccgggctc
      301 tttgccagca agctgggcaa gcagatcttc cgcaggaatg cctgcgagct tttgcggatc
      361 atccataaca gcgacctgcg agcgcatgcg atcaagcact gtcagtccca ggtcaatctg
      421 gcccgcgctg agcgccagat tgcccgattg gaatgcttcg aggaaagttt cgagagcatg
      481 gcagagacgt attacccctt cgagctcaaa aatgcctgcg ttacccttaa tagtgtggat
      541 tgcacgcagc atctcggtga aatgttcggg attgtgacca cgctccagtt caagcaacag
      601 cgcctcggaa cgctgaagca gatcacgtgt cgttgtgaca aaggactctt taacttcttt
      661 gaggttaatt tccattagtt aacctccgta aggcgggtaa aagcggagct catattgagg
      721 cggcgcagca tctgcgcgag cctgggagaa acttcaagct ccatggccac gttctgcgtg
      781 cgcagtttga gccccaggga tacgaccaca gaaagcgtgc gcagcgaaag atcaccggaa
      841 tcataaagcc gcagggttac ctggtttacc ggatgccctt ccagtgcaaa gagcaggtca
      901 ccccagagtt ttgaagtctc ggccagaaaa tccggggctt ccagagtaat gaaggcagtc
      961 gttttctttc tgaagaagtt catttactct ctccgcgggc aggcacctta acatataaga
     1021 ctcgaagcgt caattctgac gactaccata ccgactaggt atagctacca acagaacacc
     1081 cggccccggg cccaaaatac cgacctcccc ccttgcagcc ctgtagaccg ccactatttc
     1141 cactgaggat cctgatgcta aaagcagact ccaccagcat acgagtatcg gtcttcatcc
     1201 acctgaactt gcgctggccg accacaaaaa ccctcagtag ttctacataa gggctgcggc
     1261 acgcttgcga attgattttt ctggagtttt gggatacaag cctctatgcc tgatagcctc
     1321 taccagtcga ttccggttct tcttgtcgat gacgacaggg cagtaagacg cagcatgcgg
     1381 cgcttactcg agagctgggg ctgtaccgta gacgaagccg gcgacgggca tgcggcactc
     1441 gagaagctgt gtgtacccgg aaggcacctc gtgatgaccg acatcgatat gccatcgctc
     1501 gatggttttg atctgctcga caccatcaac aacaggtttc cgcattgcgg agtgatcatg
     1561 gtcagcggca tgtcgcacga gaaaattata gacgcagcac tcgaacgtgg ggccattggc
     1621 tacatacaaa agccctttcg cctgcccgaa ctgaaggcgc aggtcacgaa tgccctgcgc
     1681 aggatgtcgc aggatcaggg tattgaatcc gcagacaggg atgccgcccg cgagtttgcc
     1741 cgcatgttcg ccgccactgc agacctgcac catgtcgaga cgggcgacca catcaggcgc
     1801 atcgggcgcc tgagccgcga actagccctg cgtgccggcc tgccgcatac ctctgcagtc
     1861 ttgctcggcg aagcagcaat actccatgat atcggtaagc tcgcaatacc cgatgcaatt
     1921 ctcaagaagg ccggtgcgct cacgcccgac gagtttgcca tcatgcgcac ccaccctgta
     1981 ctgggggcac aaatcctgcg cgaatccaga aatcctatgc tgcagctggc gcatgaagtc
     2041 tgcctgcacc accatgagcg ctgggacgga atgggatacc cccacggtct gcgcggcgaa
     2101 gactgctcca tcgaagcacg catagtggga atagtcgatg tctacgacgc cctgacagag
     2161 aaacgcgttt ataaagaggc gtggccactc gacaaggtca tcagcttctt tgaagaacgt
     2221 gctggaaccg cattcgatcc acggctggtg gagctcctgc tcgctgatat cgacaatttc
     2281 gaagcggtgc gactcgccgc cgacgacgta tctgacgtat acctgcgacc ggggctgcag
     2341 gttaaggtgc aaacgggagc cgccattgca gtctgaattc caggcgggta tgcagccagc
     2401 gagcgccgga ggcccggttg caatgacggc cgaccgtagg gagggggagc gcctcagtgt
     2461 cctgcggcgg attattatta ttgccggcaa aaaaagatac gacggctaca ccatcgatat
     2521 ctcacggggc ggtatttcct ttatcgccgc aggggaaatc gtctgcggcc ccgcagagct
     2581 gcaaattccc gagctgcgca gcttcctgaa gggcaatatc gtggatatgg aagagagtga
     2641 cccacgaagg ctaaggcggt accacatgac atttctttct ccgatggaga gcagcgaaat
     2701 cgagcttatt accgccattt aagtgcactt tactggtatt gctccgcaac ctgccggcca
     2761 tttaggcttg tagcagtgtt tcgcttacct atgctgcctc tgtgtcacgc tggcgtacca
     2821 agtttacacc cttgtttctg acggttttgg gtgtcgtcta tatctccgat ctgctttcca
     2881 agctctggat actgaaaaaa ctgggtggaa ttgggcagag tgaaacaatc atcagcaact
     2941 ttctctattt cacgctgaca cgcaacgaag gtggagtctt cggcatgatg cagggccatg
     3001 cctggctatt ccagattctg actggtttcg cgatcatctt ccttctttac tactattaca
     3061 aaaccccgga aacctcgctg atattttcgt tggccattgc ggcaatcctg ggcggggcgc
     3121 tcggcaattt caccgatcgc ttctatggca ccacccccgg agttgtagat tttatcgata
     3181 ttcgcctcgg ttcattccgc tggtttattt ttaatattgc tgatgccttt atctgcgtgg
     3241 gagctttcct gctcgccata gcattctacc agtttgaaaa agctgaaaaa gcagcagctg
     3301 ccgcagcgca gcaggaaaaa gcctgacatg ccagccacgc tgattgtagg cacccagtgg
     3361 ggtgatgaag gcaaagccaa ggtcatcgat tacctggcca aagagaccga cattgtcgtc
     3421 cgctaccagg gcggggcgaa cgccgggcac acggttaagg tcggtgaaga caagtatata
     3481 ttccggctga tccccagcgg tatactctac ccaaaaacca tctgcgtcct cgggggtggc
     3541 atggtgattg atcccgacgc gatgttcgac gagatcgccg agctcgaggc gaaaggtatc
     3601 cagccgacag gtcgcctgcg catcgcagac aatgcccacc tgctgatgcc gtaccacaaa
     3661 gaaatcgacg tgcgcagcga agaagcatcg ggcgcacaca agattggcac gacgaagcgc
     3721 ggcattggcg cctgctattc tgacaaggta aaccgccagg gcattcgcat cggtatgctc
     3781 tacagcgatg aattttttgc cagtacactg ccacgcctcg tcgaacagaa gaatcgcctg
     3841 ctgacgaaga tgtacgatgc cccgccaatc gatctggccg cgatgaaaga gaagctactc
     3901 gcgctgcgtg aaaaactgcg cgatatggtg gtgaacgcgc cctactatct taacatggaa
     3961 ctcgcttcgg gcaaacgcgt gttactagaa ggcgcgcagg gcaccatgct ggatctcgat
     4021 ttcggcacgt acccgtacgt caccagctca aatcccacca cgggcggcgc cctttcaggc
     4081 agcggagtat cttttcaata ccttaagagc gtgatcggca ttgccaaggc ttactctacc
     4141 cgggtaggcg aaggtcccta cccgactgaa tgtttcggcg aagagggaga gattctgcgg
     4201 aaggcgggca acgagtttgg cagcgttacc ggccggccac gtcgctgcgg ttggtttgat
     4261 gccgaggtcg tgcggcacgc ggcgcgcatt aacggcctga cctcaatagc actcaccaaa
     4321 atcgacgtgc tggatacctt caagacgatc aaggtcgcaa cgggctatga actcgatggc
     4381 aagcgtatcg actatttccc gtcgtgcaat gtcgccaacg tcaaagtcat ctacgaagaa
     4441 tttccaggct ggaacgaacc cacttcgcac attaccaagc tgaagaatct gccgaagaac
     4501 gcccgccggt acatcgatgc aattgcaaaa ttctgtggcg tgccgatcgc ctgggtgtcc
     4561 gttggtccgg gccgcgaaaa caccatagcg atcaagtaac gcaatgaccc gcgttagcgt
     4621 gcgttccggt aaacccggca ggcaaacacc cgcatgacgg taaaggagct cttcacttac
     4681 cgcatcctca ctatctcgaa cctgctttca ttgtcgcgca tcctggggat accagcgatg
     4741 tggtatatcc tcgcgcatcc acacgatatt agcagcatca ggctctacac agcgctgacg
     4801 cttttcttca tgacgctcac cgattttctc gacggttacc ttgctcgccg cctcggccag
     4861 gaaaccccgc tgggtcagta cctcgacccg atcgccgaca aaattgtcat catttcgggc
     4921 ctgggattac tcgtctatta ccgcgacttt ccgctgtggg taacaatctt cgtgggcctg
     4981 cgcgaaatta ttggtaccgt tggcggcggt tacctgctga tcaagcgcaa tgtactcggc
     5041 aaacccaatt actggggcaa aacaggcgtg gcaatggtct cgctgtccgg cctcttttac
     5101 ctctttaact ggccctaccg cgagtggaca attccgccgc tcatcgcggt cttcgccggc
     5161 ggtattttag cctacgcgaa aacctatgga cgcacgatat ttaaagggag tgaacacggt
     5221 taacctctac atctacctcg cggcggccgt gcccctgcag ctcggctttg cggcgatcta
     5281 ctttgcggtc atgcattttt ccccgcaaaa tgtgctcctg cgcgaagcgc tgcccgtagc
     5341 ctacctggca gtaacgtacc tgcagtggct tacattttcg ttctggattt cgctgcgcag
     5401 taaaaagtac cgtgttccca tcgttatgca cctgccccac ctgctgagct tcatgccgct
     5461 catcaggcat atcatgctcg ctgccgaccg ccaggcagtt gatgcaacga gtctcaacct
     5521 tgcctggata cattcggtag tgacacacag tttctcgctg ctggcagcct ggctttggca
     5581 ggtgcgcgca gagttcgacg atggtaccgg cctgcgccac cgaatactgc cgatcgccct
     5641 ctatatactg gcagcgctga ccacgcttgt cgtctacgcc ggtctcgatc tcagtttcgc
     5701 cagaacgaca ttgccctttg gcctcggact ggtgcagcta ttgctttata ccagcgttac
     5761 cctgctctta ctgctgcgcg aaaaaatacg ccatccgctg cctgcaattg cagctgccgc
     5821 tttcgctatc gtgaatgcca tcctgctctt tgaccaggca cggtcagata ttggggtaga
     5881 attcgcattg gcggcgggca gcgccaccca gctgttctcc ctctatgtct tcctcgtatt
     5941 ctcggcgcac cggccgctgg ctaaacgctg agtggtttat tcccgagttt tagcacaccg
     6001 cgcggacaca ccgacatgca gagcccgcag ccgacgcagg ctgcgcgctt aatgtcttcg
     6061 ccgcgcatcg catacgacct tacatcaata cccatttcgc agtttgcgga acaactgcca
     6121 caggcaatgc actgcccgcc gttggtcgaa atgcggaagc gtgagaaaaa tacctgcaga
     6181 atacccagta tggccgcctg tgggcagcca aaccggcacc agacgcgcgt acccattagc
     6241 gggtagaagc cagtgccaat cacgccggca aaaccagcgc tgataaagaa gccgtaccag
     6301 cgcttcatcg aaaagaccca ctgcgcaaag ccgtcattaa taccgcgaaa ctcgttaata
     6361 aagatcaggc cagtcagtac cacggtaatc gccagaatgc tgtagaccgt gatgcgttca
     6421 atcatccagg cgcgttctga tttatctgac agctggcgaa agggatcacc catcgtattg
     6481 gccagcgcgc cgcaaccaca gacccacgaa caataccaac gcttgccgaa aaaataggtc
     6541 agcaccggca ccgcaacaaa ggacatcatt acagcccaga gcgccatcgc ccgcggataa
     6601 tcggtgaagg ttgaagggag aaaatactcg agcttgagcg gccagaaata gctgaaatag
     6661 aactccttcg tattgaaata gagcagcagc gcggggagca tgaatgcgat caccgactgc
     6721 accgccatga cggaaagtgt ccggatcttc tggtagcggt cgtgccgcca ccgccggtag
     6781 aaacggattc ccatcgaaac gaccagcaag gtgtagagcg cgccatagag aaaccaccgg
     6841 tccgagtcgc ggttgcgcag gaaatgggag acgggattga gtaccgccga ggttctatcg
     6901 acccagctcg tcacgggcgc cgggctcgtg tttctcagcc gatcgacaaa cacgcggtat
     6961 tcgggcagca ggtagcggtg gtagccgccg ccgaatgctg cctgctcacg gtagctggca
     7021 aggatgtagg acttctggtc tttcagaccc tgctctgcag cgattcccct ggctgctgaa
     7081 attttgcgct caagcggtgc aagcacctgg tttacgtgcg aagctgccca cagctggtca
     7141 atctcgcctg accagtacag cagcacatag aagctaatga gcgcacacga aaggataatg
     7201 ccaagcagcc ccctgcgcgt caggctctgc cagagcagca tcttcagcct gtgtagtttc
     7261 tgcattcctg gtgtatcggt cgcagtaaca gcagtcatac ttcagcacca atgtgcgcgc
     7321 cgcttcgtct gtaaagattg ttcatctttc ggcccgggac aaggcaacca ggctacagcg
     7381 gctcaaagtg cagcggcagc gggttcttca gcgtcgaggt gaacaggtaa ttgatctgcg
     7441 gctcattgtt gtctggcgca gagatacggt agcgccgcag tatgcgggca atcgccacct
     7501 gcatctcgag catcgcgaac atgctgccga tgcaccgccg cggccccgcc ccgaatggca
     7561 tgaatgcctg tttttgaact ggctccgggg tttcgccgag aaagcgctct ggccgaaagc
     7621 ttgccggtgc agaccaatac tgcggattgc ggtgcagcgc gatgataccc gtctgtagtt
     7681 gagagtcttt ccgaagcagg taaccttcga attcttcgtc ggcaacaatg cggcgaccga
     7741 taatccacgc agcagggtat agccgcaggg tttcattgac cacggcttcg agtagctgtg
     7801 atttctccag tccgtcgacg ctttcccctg cgccatgcgc tgaaatttca tcgcggagat
     7861 tttcctgtac ctgcggatgc cgggcaatga gccagagcgc catggccagt gtgaaacctg
     7921 ttgtttcgtg accggcaatc agcagggtga gaatttcgtc gcgcacctgc gtgcgcctta
     7981 tcttaccctg gttacgctcg cgctccgctt tgagcagcat gcccaggagg tcgctgcctg
     8041 catcgctgcg ctgcgcacgt tcatcgatga tgcggtagag tatctggtcg agctcgaagc
     8101 ggtgccacaa aaaacgcaga ttattcagcg acggaaaata gactggcagc ttcagcggcg
     8161 cctgtatcct gctgctgata aaactgttac cgaaatgcac cgcctcacgt atcgtgcgct
     8221 cattggctga gaagtcctcg ccaaacagga accggcagat attctccagc gcaagatgca
     8281 tcgcgagcgc cgagacatcg gctacactgc cgggcacagc aatagcccgg tctgagacat
     8341 ctaccatcgt ggagacaaaa cttcccagcc ggctcctggt gaaggccggc tgcaccattt
     8401 gccgctgcga ctgccaaaaa tcgccttcgc tgttcaaaat tccgttgccc agaaagtgcg
     8461 ccgcttcttt gtaagcgtcg gctttggcgt atgaacgcgg ttcgctcaca aaaactttgc
     8521 ggatcagcgc gaggttattc gtgatataaa aacgcccgat acccaggtca gaaaaaacca
     8581 ggttcccgta ttcgcggtgc aggtcttcaa agtacttgag cgggttcgtg acaagcaaag
     8641 gtccgcctgt cgcgaaatca gcgatcaagg acaggggatt tactttcggg ccgggaggta
     8701 ggactgcctg cactgtcatg gtcgacgacc ggtgatgtgc aggctgcgta aactaaaaga
     8761 ctgccgtttt agccgaaagc ggcgagcagc tttttaccaa aggcaacatc cagcgcatga
     8821 ccgccgcgcg caccgagtat atgcccctgg atcggcctgc caaccagcgc aaggtcgccg
     8881 atcaggtcga gtatcttgtg gtaaaccgac tcgcgttcaa accggaaatc gttgagcgtc
     8941 gttccatcgg gcatatagat cactgcattg tcgacgctac cgccctgcgc gaggccccgc
     9001 gaccgcaggt actcgacgtc tttcatgaaa ccgaaggtgc gcgcaaagcc aatagtctca
     9061 gtgaaaacaa gatcctcata accgagctgc actgcctggt tcttcagcaa cgggtgcggg
     9121 taatctatgt aataggagac ctggaattca cgcgccggca tcgcaacaag gtatcgttca
     9181 ccatcggtca ccgatacggg acgcgtcaca acgagtggct tgacgcgttc ggtgtattgg
     9241 tggagctcga gctgctgcag gctgcggatg aaagggccgg cgctgccatc ggcaatcggt
     9301 acttctttcc caccctccac ttcgatcgca atatcggtaa taccgagaac aaacagcgca
     9361 taaagcagat gctcgacggt ctgcagctgg aaactgtttt cgcctgagcc gagtgtgacc
     9421 gcgagcgagg tatcaatgac attgtccagc gtggcggtaa tcgctgtccg ctcggtgcca
     9481 ttttttccct cgcggaagaa gacaattccg tcaccgcagt ccgcgggata gaaagtcagt
     9541 ttggttacct ctcccgaatg cagaccgaaa ccttcgtaag tgccaggatg tttgattgtc
     9601 gtgcggtaaa tctgaggcat ggttgatgct atttcctggc gcggaccttc gtcttgcgcc
     9661 gcgccgccac ccgcttctgc ttttccgaac tgctgatatc gggttcgaag aatataaaac
     9721 gcacggccgg aaactgctgc ctgatggatt tctctatagc attgatttga tcgacgagcg
     9781 cacgtgcgct caccggccag cgacggaaag caaccttgag cgcgagtacg atgtctggtc
     9841 cgtgatgctg ggtgatgaga ttcagcacct gtgcgtgggg atatttttcg gagaagaaac
     9901 tccgtagctg gcctttgaat gcttcgtcgg cgctttcgcc gataatcagg cttttgatct
     9961 cgagcgttat catcagggca atggcgatca gcagaatacc gatgacgata ctacccacgg
    10021 catcaaacag cggattaccc gtcaccagcg ccagcgatac gaaaaggagc gcaaccacca
    10081 ggcccataag cgctgcaata tcttctccga tcaccaccat gagctcactc tgccgggttt
    10141 cgcgaaacca gcggtagagc gagcggtgac cacgcgaggc acgtacttct ttcagggcgc
    10201 catagagcga aaacgattcg agcgcaacac cgattgccag cacggcgatc gcaagccagg
    10261 ggtactgtaa cggctctggt ttgagcagct tatggctgcc ctcgtagaca gaaaacaaac
    10321 cgcccacgga aaaaagcagc agtgctacca ggaaagacca gaaatacgag accttgccgt
    10381 aaccgagcgg gtgcagttct gtcgccggag ccttggcgcg cttcataccg aacagcagga
    10441 gtacctgatt gccacagtcg gcgaaggaat gaatcgcctc tgccgtcatc gaggtagaag
    10501 cagtaataac agctgcgaca gttttggtca gcgcaatacc tgcattcgca gccagcgcgt
    10561 aatagatcgc cttggttccg gtagccatgg attatatacc tccgaatata aaaatctgca
    10621 tgacgtcgcg gcaatgattt atgcgctcgt cggatacagg atgcgcaact taccccggct
    10681 tttgatcacc atcacgctca tgcagtgctt ttttgcctgt cgccgtgcgg aaaacactga
    10741 gcaccccagg ggagcggccg aagcggaaag acttgcggca attctcgagt ctcccgatcg
    10801 tgcacactgg cagatgcctg accgcgtgat cagtgaatta cggctgaaga acggcgaagt
    10861 tgttgccgac attggcgccg gcaccggtta cttcgcgcgc cgcattgcgg aaaagattgc
    10921 ccctgccggt ttgtccgtgg gattcgatat taatcccgac ctcgtggcgt tcatggaacg
    10981 cgacgccgca acgcgcggac tttctgcaaa ctacaaggcg cggctcattg cgtcgcgcaa
    11041 ccctactctt gaagatggtc attacgacct ggtcttcttc tgtaacactt accagcacct
    11101 ggagaaccgg acggcgtact tcaacagcct ggcaaaaagt ctcaagccga acggccgtat
    11161 tgtggtaata gattttatca aactggaaaa gaccgatgaa gaatcgggcg aggttccgga
    11221 gaatctggtc gataaagaaa ctgtgaagcg cgagttcagg caggctggtt tcaaactcag
    11281 gcgggaactg aaattcctgc cgtcgcagta tttcctcgaa tttgtccggc gcccggtcgg
    11341 ctaatcacat gctgggtttc ttcaccaaac tcctgaaagc ccagcctaat gcaggttgtt
    11401 ccgattgcgc taagcgcgaa aaatatacgc tattgaatgc cgctgcagat acattgcgcg
    11461 gtgattttgc gagtgaccag ttgcgcgata gccggctcga aatctgcaag gcctgcgagt
    11521 acctggcgat gggctcaaac tgcaaactct gtggctgttt cgtgcacctc aaggcgcgct
    11581 acgccgccgc ttcgtgcgac atcaataagt ggtaaagctc agctcgcctg atcgagcatc
    11641 agcctttcac cgccgagcag gaaagcagcg cggccgtcgc tcagtagcgt ctcaaactcg
    11701 agaatttccc cgggatattc ccgccggcga tggtagcgca ccgcaacgcg gtcgtaggcg
    11761 ggtattattt cctgcgctgt gcgcggccgg cggaattcgc gccagagcgc ccgcgtcagc
    11821 ctgagccaga gcgaacctgc atcgtgcagc tgcccggtgg cctgcgcaag cgaagttgcc
    11881 atatcgcgca aatcaggggg gaaatcccgc tgcgcgacgt tcatgccgat gccgactata
    11941 aacaaatttt gttctgcagg aaaactctca cacaaaattc ccgccagctt acgcccttca
    12001 aacaggatat cattgggcca cttgatccgt gtacttatcc aatggctgag cacttcatgg
    12061 cacacaagcg caacgtgtgc tggcagcagg ccagccagcg aggcgtcata cggcagcaaa
    12121 atcgaaaatg ccaggttatc gccgggactg cccgagaacc aggatcgctc cgccctgccg
    12181 cgccccgcag actgcaatac ggcgcgcacc gaggtaaacg cccgcagagc cgattggcgc
    12241 tttagccagt tattcgtcga atcggtttct gccaggcaaa caaggcgccg caaacggggc
    12301 ctcagtcagt aaggtcttct ttttcccaga gaaagtagcg ggcgaaaaaa taatagaaca
    12361 gcagcgggaa gatcactacg aggaatgagt gatcagtacc ccagaccgcg caggccactg
    12421 cgagggatcc gagcagaaaa aatgcccaga acgctctgaa ccagcgggca taaattccgc
    12481 tgattgctgc gggcactccg gcgagcgaaa ccagcttgat gattccgcgc cacgaaagtt
    12541 cagccggttt ttggcggtgg agctgcagcg ataaccatgc aagatagata aacgttgcac
    12601 cggatagcgc gagcagcgtg atggtcaaag cgcccatggc ggcaggatct ctgctgcatt
    12661 caggccgaaa agtcaaaaga cctgcgtatt ccgggtagtc aaagcaggta ttcccgcagg
    12721 agatttattt tgacagggca ttttcattac cggttatgca agtatgcata atttcaacac
    12781 atacctggcg caaggtatcg ggtcactacc cagctcgctg gtatctgctg tactgattct
    12841 catctccatt ctggcactga ctgtcgcgct cgaacgcggg tatattctct tcagggccaa
    12901 cgtaccgatt aaccccgatg aagcccgcca gctgattgcc atgattgcgg cgaagaaata
    12961 cgatgaagcg cgtgatttct gctctggcaa gctgcatccg ggttaccggg ctgtccagcg
    13021 catcatcgaa ggccgcagcc tgcaccctga cctgatcggt tttgcgcgcg aggctattct
    13081 gcacgaacag accatgttgg aacgttttac ctcgttcctc ggtacgacga caactctcgc
    13141 cccgatgctg ggtctgctgg gtactgtcac tggtatgatc aaggctttca gcgcttttgc
    13201 ccaggctgcg caacggtcgc agcagctgac catcggtatt cacgaggcgc tcatcacaac
    13261 gaccctgggg ctgatgattg cgattccttc actggtcttt ttcaactatt acgtgaagcg
    13321 cacaaaccag ctcatggacg aatgcgaact gctcgtcgac cgggcaatga aagagctcgc
    13381 ctgaaatgct cacgcgatcg cgacaacgcc tgcagctgag gacgggtata gagcttgccc
    13441 cgttcgtcga tattttgttc ctgctggtaa cctactttct gatgaatgcg acgcttgcca
    13501 acaacccggc aattcgcatt gaactgccaa aatccgacac ttcgcaggcc gccgaaagcc
    13561 aaccactggt agtgctcatc aaccaggata accagatatt tgtcaacgat cgcaacgtgc
    13621 ccctgaaaga tttgggcaag accgtggcag acatggtaaa agacaaagaa aaggaccagg
    13681 taattattcg tggcgacaag cgctcctcgt accaggtagt gatctcggtt atggacgagc
    13741 tgaacaaggc gggcgtcacg cgttttaacc tcgcgactga caaataaacc atggcggcgc
    13801 ggcccggcag aattgaccgc gcggtactgc tttttcgcgg caccgctttt ctgcaggggt
    13861 ttgcttttat tgtttatttt cgctcgctgc tcgacaacct ttatcctttc taccggggtc
    13921 tctttccttt atcgcaattc gcactgctgc tttttgcgct gggctttgtc ggtgcagccc
    13981 tctaccgccc tgcaggaact acacgtgtcc gccgggtact ggccggctta ctgctgggcc
    14041 tgccggctgc aggcagcctg atcgcgctat accatttcgc aggccgcgaa cgagcgctgc
    14101 taatattccc cttaccagtc accgcggttt cgctttttct ctggtacgaa ctttcgcgcc
    14161 acactttcgt atttcaaccg gcgaaaatct ggctgatatt cggcgcgctt gccgcttttt
    14221 ttgcaggtgg tctgcgggat ttcccgatgg ttgcacctgc ctgtgctttc accgccattt
    14281 tactggcgcg cctgctgtct gctcctgagt ggcaggtagc acatgaacgc atagtaacac
    14341 tgaggcaact gatcgatttc ctgcgttacc tctttctcgc gcaggcattc catggtgtgc
    14401 tcgggcaaaa ccgcagcaat ctcgtggcgg tcgtctcgct ctgcatggcc gggcttgcga
    14461 taccgcaggt catccgtctc gcaggtacgc gccgccccca catacagagt ggactgctga
    14521 gcctgcccct ggtcttcatg ggcctcgcag cactctacaa tgcgttgcat tataatttgt
    14581 ggggagctct cgcttatacg ctgcttgcag cgtgggaagg agtctacttc gcccgggcac
    14641 atgaagtcta cctgcgccgg gaaaaaatcc tcgccggtat tgccatagca gccggtcttg
    14701 tcgcctatta tatttcgacc gaatggctgc agattctggc cggtagcctc gtgaccctgt
    14761 tcctcgcggg cattctcatc tatgtcgcca agaactggcg gaaagccata acggcactct
    14821 tcgcgatggc ggtgatgggt tggttgctgg cattgcagtg gaagtatacg cattcggtaa
    14881 cccgtgattt ctaccggccc ccggtaacaa agctgcgcgc agcccagcta cctgacactg
    14941 gcctcgtgat gactatcatg aagctgcaga aaaataccgg taaaactatc aggaccaaca
    15001 tgctgcccga tgagttgctc gctgatgcgg cgtggcgtac cgagaatatc tcaactttta
    15061 atgcagaccc cgccacgctg gtgctgcgcc tggcatacga ttgcgacttc agacgtgaag
    15121 cagctgtata catatttgac gaaaagagcc ttggcatcta tgcggaaagt caggcgttaa
    15181 tggtactgcg agagtatttc atgcgtgtgc cccattgcca gatgttcctg gccgatggac
    15241 aagcactgcg tgaagtcaca aatctatcgc aggttctgca gtcagatccc ggacaggcgc
    15301 aaaacctggc acccgaggat gcggctaaaa ttctcggcct ggcacgggtg gtaaagaagc
    15361 gcgacctcac tgccgaatcc cagggtctct atgagcaggt attccgcttc taccgggaag
    15421 acccggtgtt cctgcgggaa ctatcgacac tcgcggcggc ccgcggccag atcgacaggc
    15481 agattgaaat tctgaacctc ctcattaccc tgcgcaagga caatacgatg tacgacaaga
    15541 agctcctgat ggagctctac gctatacgta acgagcggaa gaagagtgcg gccctggcct
    15601 atgaaattct tgctgatggc agtgaatcac cgcttgccat gttcactttt ttgcagaaac
    15661 ttttttcaga gccatttgac cgcggcgaaa tgcagtcgct ctaccagaaa attgcgcaat
    15721 accagccccg cacagacctg gagatcatta agtatgcagg tttgaaacgc tctattgaag
    15781 accgcctgaa gcagaacccg acctacgacc gcaagttcat ggatgaaaac caacgccagg
    15841 aattcatcag ctttcctgag tagatactca gtgtttcttg cgattcatac gctctgaagc
    15901 atcgcgcaga aatttcttct cggccgcagt aaggctcttc atgccgtcgc gggaaatttt
    15961 gtccaggatc acgtcgatac gcgcttccat gtctcctggc tgcaccggtg ctccgtgatc
    16021 cgcagcaggg cgtcgtattc gactactaaa aaaattcccc aatgaaccca aagcacggcc
    16081 cacacgctgg ggttcgccac ccgtcaccag cagcagcaaa ccggcagcta taagcccccc
    16141 aaggtgaccg gctgcagaaa ggacaaaagg tgccgccgaa accgcataag aaagtgcgat
    16201 gaaaccagta attagcgcga caaatttcat gcgcacggga aatagaaaga aaaaccagac
    16261 ctgacggtca ggccagtaga tggcgtaagc gatcagtaaa ccgaggttcg cacccgtcgc
    16321 cgacgagaac acatcgtatc gcatgccggg aaacagcgcc acgagcagaa ggaacacgag
    16381 tgccgaggaa acagcacaaa aaaggtaata gcgcaggaat gccagccggc cccagcgctg
    16441 ttcgagctca gaaccaaaca tccagagcgt cagcaggtta aaaagcagct ggaagggatt
    16501 ggtatgtacc agcgacgcgg taaccagctg ccagaggtaa cccttgccga ttgcggttgc
    16561 ccagctaagt tcaaatatct ggcgcagtgc actccggtgc atggctgctg tctctatgat
    16621 aatcgcgatg ccaagcgcga tctgcatcca gatcaggcgc atgacggtcc atgtggctcg
    16681 tgggggagaa tacattttac tccgattggg tctggcgaac gaggaccatg tgatcaatct
    16741 gcccccagaa cataaaacca tttccaccgc catccgcgct gcctttgatc tggtcggcaa
    16801 agtatttttt gccataaccc cacaaaccgt tcttcatggg aaacgcctgt atgtacgggc
    16861 ggaggataaa attgccctgc gcgtagaaac ttccgcgcga aagggcaagc aacaacaact
    16921 ggtaggcacg gtcgtcgcgc gcgtattcgc gcatatagtc atctccaaag tgggagctgt
    16981 agtgcatcgg cgatatgaca tcgagaatac ggccatagac ctcgagatcc tggccaatag
    17041 caccgggaac gcggtacatg ccgttataac cgtatatatc ggcgcccagc ggcaggtgcg
    17101 tctgctgcct gataccgacg agaaaatttt ccagcgcctc tgaagcgtag tggtcgctct
    17161 tcttgtgcga gtagcggcag cgaccgatag cgccgtccga tgggaagcgg atgtagtcga
    17221 gctggatttc gtcaacgccc cgctcggtca gctcccgcac gatcggaccg tagtactcgg
    17281 tgagcaggtt aggattgtac gggtcgatcc agcgttcttt gggagatcca atccatttgc
    17341 cgccgccggc atcacggatg gcatacccct cgcgttcaaa gagtttggga tccttaaaga
    17401 tcacgaggcg cacgacaacg cgtttgccca gcgcatgcac cttttcgacg acatcgcgaa
    17461 tcttgtagag cggccgcact gcgccgatcg ctcgcgccac ggggacgtca gaatcgtaac
    17521 gaatgatgcc attgtcgtcc ttgccgtcga tgacaagtcc agtccagaaa ttctgcgtaa
    17581 gctcacccaa aaccttgggc tgtttctttt gccaggtaaa cgccgagctg tagagcagac
    17641 gctggcctga tgcattctgc atacgcgcct gcttttcggc actgagacgt tgcttcggtg
    17701 ccgtgaaaga cagacgattt ccttcggtat cgagcagtgc ataagctgca tagtctgcga
    17761 catcgccaac taccttggac tgaggcgtaa aaatccagcc gccgccggtt gccggcccct
    17821 gccagatacc gcggcggcag gaaagtacga tattggaggc tccggttgag ctgatggaga
    17881 aacattcatc catctcctca ccgagcatcc ccgtatcaat cttgcggaac ctcgcggcat
    17941 tcgcttcttt caggtagact gccgggcgcg gaccgctggc aaccagcagg tggccttcgc
    18001 ccgtctcgtg aatcgcctgt acctcttcgt atatgaagtg cgatttatta tgcgctttgc
    18061 ccggcaagcc ggcgttgagg ctcgcaaatt ttagtacaag cttgccccca ggcttctgcg
    18121 cagacttcaa cccgggctcg ctcaaacggg caacttcgag accgttgaca gaggttccca
    18181 tatacaggtt ttctttgccg cggaataagg cggtaaaaat taccgcgcgt ttcaggttgg
    18241 tttttactgt ctgccagcgc tgctgcgggt cttcggtgcg aaacaacagg tcgtgcttgt
    18301 gcgtcgcaaa ttcgagcgtg ttatcaaccg cgtacacgcg gcgctcgttg gcggggtcga
    18361 gcgccatagg tatccggtgt gcgtggtgat gcagccaggt tcggccctgg tcctgtgatt
    18421 caaaaatacc acggttcgag gtgccaacga agaggcgcga ggcgttggcc cccatgacgg
    18481 tgatatcggt ttcagctgtc agcaacg
//
LOCUS       DAOQJF010000013        18316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1420_length_18316_cov_5.164120, whole genome shotgun sequence.
ACCESSION   DAOQJF010000013 DAOQJF010000000
VERSION     DAOQJF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 18316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18316
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_18316_cov_5.164120"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1035
                     /locus_tag="PLF85_01425"
     CDS             76..1035
                     /locus_tag="PLF85_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18343.1"
                     /translation="MLTQIATYLFIAALVSLPLFAETGENTAAKQEAKDPDRRLPIVN
                     PLFAPYQGQWYVEANSTEQRSAITATIPVQNYSGFAISESKTRSDYTDIRVGGQVMYG
                     IHDRLAVGVRTRFLADRQVQTSSSGAFTSGSSGVSSRLGMYNPSFALQGRLLGIDRNE
                     WHLNVIGMYSPGSVGGIGDPQTDVQAGLLLGKNSGFFSFGFVAFAIWSPETTSGTIKY
                     RSTTAVSAEMILQAHFSPFFFNLLGGAFQYVDPSSASDAMSGKVRIVCEAEVGAEIQQ
                     NVFVKLSYGWLMPVSADYNSGGLQMTFRDHGGPAATLTIGARF"
     gene            1156..3870
                     /gene="acnA"
                     /locus_tag="PLF85_01430"
     CDS             1156..3870
                     /gene="acnA"
                     /locus_tag="PLF85_01430"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNE18344.1"
                     /translation="MTNPSWADKLGTRKKLQTAAGEVAYYSLPELAKKYPNINRLPYS
                     IRILLEAALRQEDGFIIDENHIKTIAEYNPKSVKEEEIPFKPARVVMQDFTGVPGVVD
                     LAAMRDAMTELKIDPKKINPVLPVDLVIDHSVQVDFAGSPDALDKNNKLEFERNGERY
                     EFLRWGSGAFSNFQVVPPATGIVHQVNLEYLAKVVQTRTHNGELVAYPDSLVGTDSHT
                     TMINGLGVLGWGVGGIEAEAVMLEQPIYMLIPEVVGFKLTGKLPEGATATDLVLTVTQ
                     MLRKHGVVGKFVEFYGEGLASMSLADRATIANMAPEYGATMGFFPIDDETIRYMKLTG
                     RDEKLCDLVEKYAKAQGLFLTKEAPTPDFSSTLELDMGKVVPSIAGPKRPQDRIELKN
                     AKADYRKAMADVFKDAPDKTVDVSLNGKTEKLGNGSLVIAAITSCTNTSNPSVLVAAG
                     LVAQKAEKAGLRVPATLKSSLAPGSRVVTKYLEAAGLQKSLDAIGFNTVGYGCTTCIG
                     NSGPIDAALSEAINKNNLTVGAVLSGNRNFEGRIHADVKANYLASPPLVVAYALAGTM
                     DIDFESEKIQGKVMLKDIWPSQQEVNDALAKAVTSDLFKKEYGNVFKANEMWNNIKVG
                     AGSTYQWDQKSTYIAKPNYFENFSLKEPGIPSLKDIRCLAVFGDSVTTDHISPAGNIK
                     KDSPAGRYLTGRGVQPVDFNTYGARRGNHEVMVRGTFANTRIKNLMMAPDAVSAKVAD
                     WTKVPEGGNTVHIPSGEKMSIFDAAMKYMEAGTPLIVLAGKEYGTGSSRDWAAKGPAL
                     QGIKVVIAESFERIHRSNLIGMGILPLQFKDGQNAQSLGLDGSEVFNIEGYDNNLKPR
                     TDIKVTATKKDGSVVTFTTMNRVDTPVEVVYLKNGGILHTVLRKLGAG"
     gene            complement(3917..4294)
                     /locus_tag="PLF85_01435"
     CDS             complement(3917..4294)
                     /locus_tag="PLF85_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNE18345.1"
                     /translation="MKIIASNQAPTAVGPYSQAIAAGDYLFLSGQIPLVPETGLLVSE
                     EIKAQTEQVMKNLRAVCEAAGGSLSKIVKCTVFMTDLAQFQAMNEVYAAHFGEHKPAR
                     STIQVAALPRGASVEIEAIMYLK"
     gene            4399..6405
                     /locus_tag="PLF85_01440"
     CDS             4399..6405
                     /locus_tag="PLF85_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE18346.1"
                     /translation="MKFRTKIRLTAFLLLVPLVFLAVQFYWKSAEDVHAARLERHGLR
                     YLAPIQTAVYHLQRRRGFVATQGAGGIDNSASIRTESDAVRKAVTDFDSSHAELRDPL
                     GIEGEWTDMKAKLTYLADLPVTGDRNKQVELHNSTVLALFAFESVITERSALDLDPEA
                     GTYYLIELATRTSGPLSEALGQVRYRGTVMLQGGARTIENLAALEAQHGIIQFRTAQV
                     EQARWRVAKHLPDRKADLDRLFEAQSKAAEKFEEYHGQLISGKNAGQAAAQRYFAVTT
                     EYIDILAQLAAKINIIIGENLTTRMRLAIFKQWSILILTLAGLVLSGLAGWLIMRQIS
                     VSLENAMSVSDSLATGNLDQKIESETQDEIGAFMRSLQIMAERLKGVLQQVHDSATEI
                     SLAAGQVASTAEMLNNGAMDQAAHVEETGAALGEMVGLIQSNAKNAIETDQTATTAVK
                     NTQIGAENVLRAVESMKEISERIQIVQEIASQTNLLALNATIEAARAGEHGRGFAVVA
                     TEVGKLAETSGQAAKQIQTLLKQSSAISESAASSLTLITDSMQETARKVVAVREASEE
                     QSQAAKQISESMGRLNQTTEQTASAAEELAATAEEMSSQTAALLENLKFFRFESVAGG
                     VMSSYSAANTIRQMAQKSGVTSRNTTGAAGTEPLVINPGSYEKF"
     gene            6460..7227
                     /locus_tag="PLF85_01445"
     CDS             6460..7227
                     /locus_tag="PLF85_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE18347.1"
                     /translation="MALEISKHGRAALVRINHNAANTLTADSFEGIRDTLRSFEHDDS
                     VRAVVFAGNDQGYFSNGFEPSLFLGKDFDYNHNVCRLVNETALYFFFFPKPIIACLNG
                     HAMGAGAVFSLFSDWRFMAEKSARFGFPEALLGMNFPSFVGRYMQDLVGLTATRDILF
                     GGKSLKGPDALAIRLVDQVFPEDKVVEETLKFAEKLAKSPLQVLTGMKRARTEPYRAL
                     FESLRDEDARMLATYMTSPTVQEGLLSIVEKRRPRFD"
     gene            7232..8239
                     /locus_tag="PLF85_01450"
     CDS             7232..8239
                     /locus_tag="PLF85_01450"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNE18348.1"
                     /translation="MGNRNEDKRIAVLASGGDSPGMNAALRAAVRAALWEHYQVFGIQ
                     NGYEGLLLNDIRELTSRDMGLIINQGGTVLGTARSAQFQTREGLQKAAAILNEHRIDA
                     LCVVGGDGSFRGLMALSEFYKGQVVGIPGTIDNDIPGTEYTIGFDTAVQTAVEAIDKL
                     RDTALSHGRIFFVEVMGRKSGQIALSVALASGAEDVLIPEERPDIEAVVTRLTKGRDR
                     GKRFAIVVVAEGATLDAAHPVESPAFQLAEIIGKQIGQHIRVSVLGHIQRGGRPTSFD
                     RVWGSRTGDAAIRFIKQGLTGVFTAMRGGHITPAYLFEATQGVRAVEPEMVQLVRKMG
                     S"
     gene            8320..8538
                     /locus_tag="PLF85_01455"
     CDS             8320..8538
                     /locus_tag="PLF85_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18349.1"
                     /translation="MKRNTPTTGELLACGKVARADTYPCGHVILTVEQLRIGFSRADF
                     REFAQTVALAQANLDDREVARAEWGLYL"
     gene            complement(8562..10829)
                     /locus_tag="PLF85_01460"
     CDS             complement(8562..10829)
                     /locus_tag="PLF85_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE18350.1"
                     /translation="MHMRYFALLLAAMVFPLFAATLSGRVENDTGAPLDEAQVSIPAL
                     NRHAHTDKAGNFQFKDLPRGLFVVKVSGEGAASKTLTVDLRENDQDLKLKLDPLQFRS
                     DTVVISGHRPTDEAEIAQAVTVIEGKKLDRLRGQSLVATIEDTPGVANYSTGNFVAKP
                     VIRGLPSFRSLVLVDGMREESQQFGDEHGPNIDILDMDRIEIIRGPGSLLYGSDALGG
                     VINVSTPELPSADHSKLLEGRVVTNAHTNNPGGGLAVALAGARDKIGYRTTLSYRQAG
                     NTQTPDGPVSNSGYLNGNGSTLIGIHEKWGFLSLRYSHFNSRINLPQALTSTSGKLIA
                     DPDSEAYQHVVHHRVQARSQVQTQLARLEVDLTYQRNLRREYGHSHGGGGGGHAHDEA
                     PALELVLDTVNTEARAHHVPIGPLMGTLGLSHIFQNNRTLGEEPLIPGYSSNSYGAFL
                     YEELRFDSFSLLAGVRGDTRVLGVEANQQLGNSRETIQNSAATGSVGAVWRFTPGFNW
                     YANVGRGFRAPTVFELYSTGVHQGAGTYDIGVSTLRPETSLTTDTGFRLRGKSLRAEI
                     NGFYNQIENYIYAAPTGVQQVVDGNVFPEYRWSQGKATIYGGEAEAEFSPLNWLALSS
                     GVDVVMGRNETLAEPLALVPANRYRFGVTFSRASLAGLLNPYLTVKARYVARKTELSS
                     FERTLFSDFGDYTLVSLAAGGDFSVGGQMWNLTIGADNLLNRRYVDYLSRQKQYALNP
                     GINVYFKLSAPFDFY"
     gene            10978..13071
                     /locus_tag="PLF85_01465"
     CDS             10978..13071
                     /locus_tag="PLF85_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE18351.1"
                     /translation="MPIKILSLTISLFSGAAIFAAEPVQLQAAGCRTADFFHESDSEK
                     PVSQLGQYRQVEAFPVLLNAIYRPARDGRLLHYSVACAISLPAGVRSDHYLYLPGIGE
                     NFDIYLNGKKIHSDIHVEGDRLRVYRYTKYSNVTLPGSLLREGENTLFFHIAGYAPIT
                     PLSQNFLLGLPFRSGYTVSAERESGPGSLLLFFLTASYIFFGLYHFFFYLRWREKKYN
                     LHFAVFSLAISGYFLAFSQEAFARVSDTRYLIIASYASQMLALGFFLLYVHSFFFERN
                     RVPLYLRVAVLVDLVLAAMVVAGRDTWYQPLLTFWYFTALPQILLVFYLIGRAVKLRL
                     PDARTLAISIAIALLMIAFDMLDTLIFYSSVRLSQFACFVVVVSLVLLMANRFINIHH
                     ETDRLNQQLSHERNAFSRFVPSQFLENLGRTTAVEIRRGDTAQRQMGILFMDIRNFTG
                     IAEKLSPAESLVFLNVFMRKMEQCISQNAGFVDKYIGDGLLALFGSRDDSAADGAVQA
                     VKASRQMLQSLDFLNRYSTDFGHIRVGIGVHCGSLVMGTLGSRRRLDTTVIGDAVNLA
                     SRLEMLTTLYGLPMLLSGDVEQYLPPEMLADVRRIDTVRVKGRRQPVTVYEDFSAEAP
                     DIAEKKRRGKVEYDEAFAAYARGDFAQAVRQFDAINSGFTYDSAAQTLSRRARKLLEH
                     GAPLNWDGILPLGKK"
     gene            13084..16419
                     /locus_tag="PLF85_01470"
     CDS             13084..16419
                     /locus_tag="PLF85_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE18352.1"
                     /translation="MTAVREGMMVSVLRSLGAFAAFLAFYIFAGVFVLRYLTENGIVA
                     VFWPPTGIALAALLLGGRRYIPVVFIGAFFVNQLAGARIVPGLIYAGINTLEGLVAWF
                     LLTRVSKIDLALAHARDYFQIFLYGGVVAPFVGALLGAITLYLSALAPQDFWHNLQYW
                     WMGDSLGVIILTPLLLIWRRRPTQTYTSRQWIEAAVGLSALVFVGQIVFAGWSFGALN
                     AYGRGFIFFIFVSWAAMRFGRHATSLAIAILIIQIMVGLVAGRGYFAAESRSVMFGTI
                     WLYMTAISVTGMALAIYIYEARLLNHQLTLAREHLEQVNALAHIGGWEFDIAENSMRF
                     SREALRILDLPEDADPDLATALSFIDESEQAVHMARGQLAMEQGIGWDAEFALRTRSG
                     RQIWVRSQGRPISENGRVVRLIGALHDLTESRYAQIALKKRELEFRRLVETASEGIWT
                     IDEHGSTTFVNRRMAELLGYQPHEMLGTHFLEYIPAEWHGVATQQLQRRREGVSESQE
                     SVLLHRSGKKIWVMASTSPIRDEAGVVVGALAMISDISERKTIEEQLKQSEARYRQLV
                     ESSADAIVVHQSGFIVYLNEPAVRLLKAHDTGDLIGRNVLELVAPEFRTTVIERMKLV
                     NGPGGHVPLVEEKFLRLDGTTVDVEVSASGTLFNGLPATMVVARDITDRKRAEAQIRY
                     LGQHDILTGLPNRALFADRLAQTIAMADRHHGVFALLFLDLDHFKKINDSYGHRAGDA
                     FLRQVAERLTGCVRAINTVSRQGGDEFIVLLNDLAHPEDAGTIAREICERLSEPFDIG
                     EIRLHASVSVGIAVYPKDGSDADTLLRNADIAMYHAKGRGRNQYQFFSEELNRITQQR
                     LDIELALRRAIAENQFYLVYQPQLNLQTGAIEGFEALIRWRHPVRGELSPLEFIPIAE
                     ESGQIAALGEWVLRKVLSDMPQFDAAGHDQLRIAVNVSALELRQTNFADRVLKLLEEF
                     HVLPMRLELEVTESMLMEDTQLAAQSIQRLSSEGIKFAIDDFGTGYSSLSYLRTLRIH
                     HLKIDRSFISDLAHDADDAAIVRTIIALAQNLRLKAVAEGVETQEQRDFLRNHGCEIM
                     QGFYLSRPLELPAALKFLSDSVR"
     gene            complement(16420..17814)
                     /locus_tag="PLF85_01475"
     CDS             complement(16420..17814)
                     /locus_tag="PLF85_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNE18353.1"
                     /translation="MNRLKYYPLRACAVLFFTLVSGERIFAQENQAAAFNAEEAVKNL
                     RSDTDLLWLMVAGFLVFFMQAGFAYVETGFSRKKNVVNILMKNTSDFLVGSLGFWTIG
                     FSLMFGPQLVAGFGFGTPVLFDRLLEGPDGKPIAFNYAFLFFQMVFAGTAATIVSGAM
                     AERTKFISYVFFSLICSAIIYPLFGSLVWSNLFDNKNVGWLASKNFIDFAGSTVVHSL
                     GGWMGLAGTIALGPRIGKFGTGHEVKPILGHNMSMSVLGMFILWFGWFGFNPGSTGTI
                     KDGSFAIIAITTNLAACAGGVAAILTSWILFKRPDVSMVVNGILGGLVAITAGCSNVD
                     PKGAVAIGGVAGVLVVYGVLLLDWLKIDDPVGAVSVHGFAGAWGTLAVGLFANPNYGA
                     GPAGLFYGGGTAQLAAQALGVAAGFLWAFPVSLAVFTLMKYIPFIGLRVPASEEIEGL
                     DIIEHGIEAYPEET"
     regulatory      17924..18079
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         4098 a         4952 c         5206 g         4060 t
ORIGIN      
        1 ggcattctag cctggccatc tctgcacgta gccagaaaaa agttgcctgt atacgcatat
       61 gtgtgagcag gggtaatgct gacccaaata gcaacttatc tgttcatagc cgcgcttgtg
      121 tctctgccac tctttgcaga aacaggtgag aatacggctg caaaacaaga ggcaaaagat
      181 cctgacaggc gactgccgat agtgaatcca ctgtttgcgc cttatcaagg ccaatggtat
      241 gttgaggcaa attcgacaga acaacgctcg gcaatcactg cgactattcc cgtccagaac
      301 tattcaggat ttgccatctc cgaaagcaag acgcgctcag attatacgga cattcgtgtg
      361 ggcggccagg ttatgtacgg tattcatgac cggctcgccg tcggagtaag aacgcgattt
      421 ctggctgacc gtcaggtgca gacatcctcc agcggggcat tcacctcggg tagttccggg
      481 gtgtccagca ggctcggtat gtataatccg agtttcgcct tacagggacg cctacttggg
      541 atcgaccgca atgaatggca cttgaatgtt attggcatgt atagtcccgg atcagtcggt
      601 ggaataggtg atccgcagac cgacgttcag gcggggctat tgctgggcaa gaattccggt
      661 tttttctctt tcgggttcgt tgcatttgcg atttggtcgc ctgagacgac atccggtacg
      721 ataaaatacc gcagtacgac ggcggtatcg gctgagatga ttctgcaggc acatttctcg
      781 ccttttttct ttaatttact cggcggagct tttcagtacg ttgatccgag ctctgcgagc
      841 gacgcgatgt caggaaaggt ccgcattgtc tgcgaagcgg aagttggtgc ggaaatacaa
      901 cagaacgtct ttgtaaaact cagttacggc tggctgatgc cggtcagtgc ggactacaat
      961 tctggtggtc tgcaaatgac gttccgcgat catggtggcc cggctgcgac cctgacgatt
     1021 ggtgcgcggt tctgaccgcc gcttccgttt tctcgcaggc aaatcccgcg aaaatctgca
     1081 ggtcggctgg cggccgcgga cagctgcagc cttcaaatca atggacgccc tcactctgca
     1141 aaaaaatctt tccccatgac aaatccctca tgggccgaca aactcggtac acgtaaaaaa
     1201 ctccagaccg ctgccggcga ggtggcgtac tattcgcttc ccgaactcgc caaaaaatac
     1261 cccaatatca accgcctccc ttatagtata cgcattctgc tcgaggcggc gctgcggcaa
     1321 gaagacggat ttatcatcga tgagaaccac atcaagacga ttgccgagta caacccgaaa
     1381 tctgtaaaag aagaagaaat cccgtttaag cctgcgcgcg ttgttatgca ggatttcacc
     1441 ggtgtgccgg gtgttgtcga cctcgccgcg atgcgcgatg cgatgactga gctcaagatt
     1501 gatcccaaga agatcaaccc agtactgccc gttgaccttg tgatcgacca ctctgtccag
     1561 gtcgatttcg cgggttcgcc cgatgcgctc gataagaaca acaagctcga gttcgagcgt
     1621 aacggcgagc gctatgagtt cttgcgctgg ggttctggtg cgttttcaaa cttccaggtg
     1681 gtaccgcctg caaccggtat cgtgcaccag gtgaacctcg agtacctcgc gaaagttgta
     1741 cagacaagaa cgcacaacgg tgaactggtt gcataccccg attcattggt gggtaccgac
     1801 tcgcatacca cgatgatcaa cggtctcgga gttctcggtt ggggtgtagg cggcatcgaa
     1861 gcagaagccg tcatgctcga gcagccgatc tacatgctga ttcctgaagt tgtcggcttc
     1921 aagctgaccg gtaagctgcc tgaaggcgcg actgcgactg acctcgtcct cactgtcacg
     1981 cagatgctgc gcaagcatgg tgttgtcggt aagttcgtcg aattctacgg cgaaggtctc
     2041 gcaagcatga gccttgctga ccgcgcaacg atcgcgaaca tggcacccga atacggcgca
     2101 acgatgggct ttttcccgat cgacgatgaa acgattcgtt acatgaagct gaccggccgc
     2161 gacgaaaaac tctgcgacct ggtcgaaaaa tatgccaagg cgcagggcct cttccttaca
     2221 aaagaagctc cgacacctga ctttagctca actctcgaac tcgacatggg taaagtcgtg
     2281 ccgtcgattg ccggcccgaa gcgcccgcaa gaccgcatcg agctgaaaaa tgcgaaagcc
     2341 gattaccgca aggcgatggc tgacgtgttc aaagatgcac ccgacaaaac ggtggatgta
     2401 tcactgaatg gcaaaacgga aaagctaggc aacggttcac tcgtgatcgc cgcgatcact
     2461 tcatgcacga acacttcaaa tccatcagtg ctcgtggcag cgggcctcgt ggcgcagaag
     2521 gcagagaaag cgggcctcag ggtgcctgca actctcaagt catcgctcgc accgggttcg
     2581 cgcgtggtta ccaagtacct cgaagcggcc ggcctgcaga agtcgctcga cgcaatcggt
     2641 ttcaatactg tcggttacgg ctgcaccacc tgtatcggta actctggccc catcgacgcg
     2701 gcgctctcgg aagcgattaa caaaaacaac ctgaccgttg gcgcagtgct ttcgggtaac
     2761 cgtaacttcg aaggccgtat ccacgccgac gtgaaggcga attacctcgc ttcacctccc
     2821 ctcgttgtgg cgtacgcact tgctggcacc atggacatcg actttgaatc agaaaagatc
     2881 cagggcaagg tgatgctgaa ggatatctgg cccagccagc aggaagtcaa cgatgcactg
     2941 gcgaaggccg tgacgtcaga cctcttcaag aaagaatatg gcaacgtgtt caaggcgaac
     3001 gagatgtgga acaacatcaa ggtcggtgcc ggcagcacct accagtggga ccagaaatcg
     3061 acctacattg cgaagccgaa ctactttgag aacttctcgc tcaaagagcc aggtattcct
     3121 tcactgaaag acatccgttg cctcgcggtg ttcggcgact cagtcacaac cgaccacata
     3181 tcgcctgccg gtaacattaa gaaagattcg cctgcgggac gctacctgac aggtcgcggt
     3241 gtccagccgg ttgacttcaa cacctacggc gcccgccgcg gtaaccatga agtcatggtg
     3301 cgcggcactt ttgccaacac acgcatcaag aacctgatga tggcgccaga cgccgtctcg
     3361 gctaaggttg cagactggac aaaagtgccc gagggcggta acacagtgca catcccatca
     3421 ggcgaaaaga tgagcatctt cgacgctgcc atgaagtaca tggaagctgg cacgccgctg
     3481 atcgtgctgg caggtaagga atacggtacc ggttcatcgc gcgactgggc ggcaaaaggt
     3541 ccggcgctgc agggtatcaa agtcgtgatc gccgaaagct tcgagcgcat tcaccgttcg
     3601 aacctgatcg gtatgggcat tctgccgctg cagttcaagg acggccagaa cgcacagtca
     3661 ctcggcctcg atgggtcaga ggtcttcaat atcgaaggct acgataacaa cctgaagccg
     3721 cgcaccgaca tcaaggtgac cgcaacgaag aaagacggct cggtagtgac cttcaccacc
     3781 atgaaccgtg tcgatacccc ggttgaggtc gtttacctca aaaacggtgg cattctgcac
     3841 acggttctga gaaagctggg cgcaggctaa taatctgctc ttttagccac cgaattaatt
     3901 cggtggctaa aagctgctat ttcagataca ttatcgcttc gatttcgact gacgctccgc
     3961 ggggcagggc agctacctgt atcgtcgagc gggccggctt gtgctcgcca aaatgcgccg
     4021 catagacctc attcatggcc tggaattgcg ccaggtcggt cataaaaacc gtgcatttca
     4081 cgattttgct gaggctgccg cccgcggcct cgcagaccgc acgcaggttc ttcatgacct
     4141 gttcggtctg cgccttgatc tcttctgaaa ccaacagccc ggtttctggc acgagaggaa
     4201 tctgccctga cagaaagagg taatccccgg ccgcaatggc ctgcgaatag ggccccacgg
     4261 ccgtaggagc ctggttcgat gcaataatct tcatttgttg ttggcagcgg gcaaaaagtt
     4321 gccgcgtcaa tgaattaaag cctcaaatag cctgcggaaa agaccgaaaa tgaaacgatg
     4381 aagtctctgc tggcgaacat gaaattccgc accaagatca ggctgacagc ctttctgctg
     4441 ctggtgccgc tggtattcct ggcagtgcag ttctactgga agagcgcgga ggacgttcac
     4501 gctgcgcgcc tcgagcggca cggcctgcgt tatctcgctc ccatacagac ggcagtttac
     4561 catttgcagc ggcgccgggg ctttgtcgcc acccagggtg ccgggggtat cgacaattca
     4621 gccagcatcc gcacggaaag cgacgcggtc cgcaaggctg ttacagattt tgattccagc
     4681 catgcagaac tgcgtgaccc attaggcatt gaaggggagt ggacagacat gaaagccaag
     4741 ctcacctatc tcgcagatct gcccgttacc ggcgaccgca acaagcaggt tgaactccac
     4801 aacagtacag tgctcgcgct ctttgcattc gagtcagtaa ttactgagcg ttcggcgctt
     4861 gacctggatc cggaagcggg aacctactac ctgattgaac tggctacacg tacctcagga
     4921 ccgctgagcg aagcgctcgg ccaggtacgc taccgtggta cggttatgct gcagggggga
     4981 gcgcggacga tcgagaacct ggccgcactc gaagcgcagc acggcattat ccaattccgg
     5041 acagcgcagg tagagcaggc gcgctggcgt gtggccaagc acctgccaga caggaaagcg
     5101 gacctcgatc gcctctttga agcccagtca aaagccgcag aaaaatttga ggagtatcat
     5161 ggccagctca tttcaggtaa aaacgcgggc caggcggctg cgcagcggta ttttgccgtt
     5221 actaccgagt acatcgacat tcttgcacag ctggcggcca agatcaacat tatcattggt
     5281 gaaaacctga cgacccgcat gcggctcgcg atcttcaaac agtggtccat tctgattctt
     5341 acactggccg gcctggttct gtcaggcctg gctggttggc taatcatgcg gcagatttcg
     5401 gtctcgctgg aaaatgcgat gtcggtatcg gatagcctcg caacaggaaa tctcgaccag
     5461 aagattgaga gcgaaacaca ggatgaaata ggcgccttca tgcgttcatt gcagattatg
     5521 gcagaacgtc tcaagggtgt gctgcaacag gtccacgaca gcgcgaccga gatttctctg
     5581 gctgcagggc aagtggcgag cacggccgaa atgctgaaca acggtgccat ggaccaggct
     5641 gcgcacgtag aagaaaccgg cgcggcgctc ggtgaaatgg ttggtttgat tcagagcaat
     5701 gcaaaaaatg caattgagac cgaccagact gcgacgacgg cggttaagaa cacccagatt
     5761 ggagcagaaa atgtgctgcg tgcagtcgag tcgatgaaag aaatttctga acggattcaa
     5821 atcgttcagg aaatcgcatc ccagacaaac ctgctcgcac tcaacgccac tattgaagct
     5881 gcacgtgccg gcgaacatgg ccgcgggttt gcggtcgtgg ccaccgaggt cggtaagctt
     5941 gcagagacaa gcggccaggc agcgaagcag attcagacgc tgctgaagca gagctccgcg
     6001 atttcagaga gtgccgcctc gtcgctgacg ctcatcacgg acagcatgca ggaaacagcg
     6061 cgcaaggtgg tggcagttcg cgaggcttca gaagaacaga gccaggcagc gaagcagatc
     6121 agcgagagta tgggccgcct taaccagacg accgagcaga ctgcctcggc tgcagaagag
     6181 ctcgcggcga ctgctgaaga aatgagttcg cagacggctg cactgctcga gaacctgaaa
     6241 ttttttcgct ttgaatcggt cgccggaggg gtaatgagca gttactcggc agccaataca
     6301 atccggcaga tggcgcaaaa gagtggcgta acctcccgga atacgacggg tgctgccggt
     6361 actgaaccgc tggttatcaa ccctggctcg tacgaaaaat tctgacacgc cggcagaaat
     6421 ttggcttcac cgcataagct cccttagaga ccatgcggca tggcactcga gatttcaaaa
     6481 cacgggcgtg ccgcgctcgt gcgcattaac cataacgccg ccaatacgct gactgcagac
     6541 agcttcgagg gcattcgcga tacgctccgc agcttcgagc acgacgacag cgtgcgcgcg
     6601 gtggtatttg ccggcaacga ccagggctat ttttccaatg gctttgagcc ttcgctgttt
     6661 ctcggcaagg actttgacta taaccacaac gtctgccggc tggtgaacga aaccgcgctg
     6721 tattttttct tcttcccgaa acccatcatt gcctgtctga acgggcacgc catgggcgcg
     6781 ggggctgtgt tttccctgtt ctcagactgg cgtttcatgg cggaaaaatc tgcgcgcttt
     6841 ggttttcccg aagcgctgct gggtatgaac tttccctctt ttgtcgggcg ttacatgcag
     6901 gatcttgtcg gtctcactgc cacacgtgat attctgttcg gtggcaaatc gctcaagggt
     6961 cccgacgcgc ttgccatcag gctggtcgac caggtgtttc ccgaagacaa ggtagtagaa
     7021 gagacgctga agtttgccga gaagctggcc aaaagcccgc tgcaggtgct gaccggaatg
     7081 aagcgcgcgc gcaccgaacc ctaccgggcg ctcttcgaat cgctgcgcga cgaagatgcg
     7141 cgcatgctcg ctacctacat gacgagtccc accgtgcagg aaggcctgct atcgatcgtc
     7201 gaaaaaagac gcccgcgttt cgactgagac catgggtaac cgcaacgaag acaagcgtat
     7261 tgcagtcctc gcctcggggg gagattcacc cgggatgaac gccgccctgc gcgcggcggt
     7321 gcgcgcagca ttgtgggaac actaccaggt gtttggcatc cagaatggtt acgaaggtct
     7381 gctgctcaat gacatcaggg agctgacctc gcgcgatatg ggtctcatca tcaaccaggg
     7441 cggcacggtg ctgggcactg cacgctccgc ccagttccag acccgcgaag gattgcagaa
     7501 agctgcagcg atactcaacg agcaccgcat cgatgcgttg tgcgttgtcg gcggcgatgg
     7561 ttcgtttcgc gggctgatgg cgctcagcga gttctacaag ggccaggttg tcggtattcc
     7621 gggcacgatc gacaatgaca tacccggcac cgagtacacg atcggctttg ataccgcggt
     7681 gcagaccgcg gttgaagcaa tcgataagct gcgcgatact gcgctttcgc acggtcgtat
     7741 ctttttcgtc gaggtgatgg gccgcaagtc aggccagatc gcgctttccg tcgcgcttgc
     7801 ctcgggtgcc gaagacgtac tgattcccga agagcggccc gatattgaag cggttgtcac
     7861 gcggctcacc aagggccgtg atcgaggtaa aaggttcgcg atcgttgtgg tggccgaagg
     7921 ggcgacactc gacgcggcgc accctgtcga aagcccggcc tttcagctgg cagaaattat
     7981 tggcaaacaa atcgggcagc acatacgggt ttcggtactc gggcacatac agcgcggtgg
     8041 taggccaact tccttcgatc gcgtctgggg gtcacggacg ggcgatgcgg cgattcgctt
     8101 catcaagcag gggctgaccg gtgtcttcac ggcgatgcgc ggcggtcaca tcacccccgc
     8161 gtacctgttt gaagcgacgc agggcgtgcg tgcggttgaa cccgagatgg tgcagctggt
     8221 gcgaaagatg gggagttaga aggtttttga ccttcaattg caattgagaa tcattatcaa
     8281 ttatgtttga cgactgactc cgtgcgcgga aaatggcgca tgaaaagaaa cactcccact
     8341 actggtgaac ttctggcatg tggtaaagtt gcgcgagctg acacttaccc ctgcgggcac
     8401 gtaattctga ccgtcgagca gctgcgtatc ggcttttcac gggcggattt tcgtgaattt
     8461 gcgcagaccg ttgctctcgc ccaggcaaac ctggatgatc gcgaggttgc ccgggcagag
     8521 tgggggctgt acctctgaca acttgcccgg gaatttttgc atcaataaaa gtcgaacggc
     8581 gctgataact tgaaatagac attgatgcct gggttgagcg cgtactgctt ctggcggctg
     8641 aggtagtcga cgtaccgacg gttgagcagg ttgtcggcgc cgatggtcag gttccacatt
     8701 tggccaccga cgctgaaatc accgccggcc gcaagcgaga ccagcgtata atcgccgaaa
     8761 tcagagaaca gcgtgcgttc aaatgaagaa agctcggtct tgcgcgccac gtaccgcgcc
     8821 ttcactgtaa ggtacggatt cagcagcccg gcaaggcttg cgcggctgaa ggtaacgccg
     8881 aaccggtagc ggttggcagg tactaaagcc agcggttcgg ccagagtttc gttgcgtccc
     8941 atcacgacat caacgcccga agaaagggcc agccagttga gcggagaaaa ctcggcttcg
     9001 gcttcgccac cgtaaatggt cgccttgccc tgcgaccagc ggtattcagg aaacacattg
     9061 ccgtcgacga cctgctggac tccggtaggt gcggcataga tgtaattttc aatctggtta
     9121 tagaagccat taatttcagc gcgcagcgat ttaccgcgca ggcgaaatcc ggtatcggtg
     9181 gtgagggaag tttctggccg caacgtgctg acaccgatgt cgtatgtacc ggcaccttgg
     9241 tgtactccag tgctgtagag ttcaaacacg gtgggtgcgc gaaacccgcg gccaacgttc
     9301 gcgtaccagt tgaatcccgg ggtgaagcgc cagacggcgc cgacagatcc ggtagcggcg
     9361 gagttctgaa tcgtttcacg gctgttgccg agctgctggt tggcttcgac gcccagtacg
     9421 cgagtatcgc cccgcacgcc tgccagcagg ctgaagctgt cgaagcgaag ttcttcataa
     9481 agaaacgcgc catagctgtt ggaggaatag cctggaatca gcggctcttc gccgagcgtc
     9541 cggttgttct ggaaaatatg gctgaggcca agagtgccca tcaggggacc aatgggtacg
     9601 tggtgtgcgc gtgcctcggt gttgacggtg tcgagtacca gttcaagggc aggagcttca
     9661 tcatgcgcat ggccgccacc gccgcccccg tggctgtgtc cgtactcgcg gcgcaggttt
     9721 cgttggtagg tgaggtcgac ttcgaggcgc gcgagctgtg tctgtacctg cgagcgcgcc
     9781 tgcaccctgt ggtgcacgac gtgttggtat gcttcagaat cggggtctgc gatgagcttt
     9841 cctgatgtgc tggtcaatgc ctgcggcagg ttgatacgcg agttgaagtg cgaatagcgc
     9901 agcgaaagga agccccattt ttcgtggata cctatcaggg tgctgccgtt gccgttcagg
     9961 taaccgctgt tggatactgg cccgtcagga gtttgtgtat tgcccgcctg gcgatagctc
    10021 agcgtcgtcc ggtagccaat cttgtcgcgc gcgcccgcga gcgcgacagc aagaccgcca
    10081 ccggggttgt tggtgtgtgc gttcgtgacg acgcgtccct cgagcaactt cgaatggtct
    10141 gcagagggca gttcaggcgt agaaacgttg atcactccgc cgagggcatc tgaaccatag
    10201 agcaggctgc cggggccgcg tatgatttca attcggtcca tgtcgagaat gtcgatgttt
    10261 ggcccgtgtt cgtcgccgaa ctgctgcgat tcttcgcgca taccgtcgac gagtaccagc
    10321 gagcggaaag agggcaggcc ccgaataacc ggtttggcga caaagttacc agtcgaataa
    10381 ttggcaactc cgggagtatc ttcgattgtc gcaacaaggc tctgcccgcg aaggcggtcg
    10441 agtttttttc cttcaatgac agtgactgcc tgcgcaattt ctgcctcgtc ggtcggcctg
    10501 tggccggata tcacgaccgt atcgctacga aactgcagcg gatcgagttt tagtttcagg
    10561 tcctggtcat tttcgcgcag gtcgactgtc agcgttttgc tcgctgcgcc ttcgcccgag
    10621 accttcacca cgaacagtcc gcgcggcaga tctttgaact ggaaattgcc ggctttgtcg
    10681 gtatgtgcat ggcggttgag tgcaggaatc gagacctgcg cttcatcgag cggagcgcct
    10741 gtatcatttt cgacgcggcc cgacagggtt gcggcaaaaa gcggaaacac catcgctgcg
    10801 agcaaaaggg caaaataacg catatgcatg gaagagcgca cgctcgaatt gggctgcctt
    10861 gtcaattaaa aatgataagt cattatcaat aatgggcacg atcaattccc accagtcgat
    10921 aaaatgcaga acatcttgcg cagccgtctg cagcgtagaa tcctacccgc agataccatg
    10981 cccataaaaa tcctgagcct gaccatctca ctgttttccg gggctgcaat atttgccgcg
    11041 gaacccgtgc agctgcaggc cgcaggctgt cgaactgcag atttttttca cgaatccgat
    11101 tcagaaaagc ctgttagcca gcttgggcag taccggcagg tagaggcatt tccggtgctt
    11161 cttaatgcaa tctatcgccc ggcccgcgac ggccggcttc tgcactacag cgttgcctgc
    11221 gccatcagcc tgcctgccgg cgtgcgcagc gaccactacc tttacctgcc gggtatcggt
    11281 gagaactttg atatttacct gaacggaaaa aagatacaca gcgacataca cgtggaaggc
    11341 gacaggctgc gggtctaccg ctataccaaa tatagcaacg tgactttacc cggttcgctg
    11401 ctgcgcgaag gcgagaacac ccttttcttt cacattgcag gctatgcacc catcacgccg
    11461 ctatcacaga attttttact cggtttacca ttccgcagcg gctacacagt ttcagccgaa
    11521 cgcgagagcg gtccgggcag cctgctgctc ttttttctga cggcatcgta catctttttt
    11581 gggctctacc attttttctt ttacctgcgc tggcgcgaaa aaaagtacaa tctgcatttc
    11641 gcggtttttt cgctcgcgat atccggatat tttctcgctt tttcccagga agcatttgcg
    11701 cgcgtttccg acacacgtta tcttatcatt gcctcgtacg cgagccagat gctcgcgctt
    11761 ggattctttc tgctctatgt gcacagcttc ttctttgagc gtaatcgtgt tccgctttac
    11821 ctgcgcgtcg cggtgctggt cgacctggta cttgcagcga tggttgtcgc agggcgtgac
    11881 acctggtacc agccgttgct gactttctgg tattttactg cgctgccgca gatattactg
    11941 gtcttttatc tcatcgggcg tgccgtaaag ctgagattgc ccgatgcccg cacactggca
    12001 atcagcattg cgattgctct ccttatgatt gccttcgata tgctggatac gctgattttt
    12061 tattccagcg tgaggctgag ccagtttgcc tgctttgttg ttgtcgtttc gctcgtcttg
    12121 ctcatggcaa atcgttttat caatattcac catgaaaccg atcgcctgaa tcagcagctt
    12181 tcgcacgaga gaaatgcatt ttcgcgtttc gttccttcac agtttctcga aaaccttggc
    12241 cgtacgacgg cggtggagat tcgccgcggc gacaccgcac agcggcagat gggtattttg
    12301 ttcatggata tccggaattt tacggggatt gctgagaaac tttcaccagc tgagagcctg
    12361 gtctttctca atgtgttcat gcgcaagatg gagcagtgta ttagccagaa tgccgggttt
    12421 gtcgataagt acattggtga tggcctgctg gcgctctttg gatcccgcga cgacagcgct
    12481 gctgatggcg ctgtgcaggc agtcaaggcg tcccggcaga tgctgcagag cctggatttt
    12541 ctaaatcggt acagcaccga tttcggccat atccgcgtcg gcatcggtgt gcactgcggt
    12601 tcgctggtga tgggtacgct cggcagccgg cgtcgcctcg ataccaccgt catcggcgac
    12661 gcggtaaatc ttgcctcgcg tctggaaatg ctcacaaccc tctatggatt acccatgctg
    12721 ctttcaggtg atgtcgaaca gtacctgccg ccagaaatgc tggcagacgt acggcgtatc
    12781 gataccgtca gggttaaggg tcgcaggcag ccggtaacgg tatatgaaga tttttcggct
    12841 gaagctcccg atatcgccga gaaaaagcgc cgcggtaaag ttgagtacga tgaagcattt
    12901 gctgcctatg cgaggggcga tttcgcccag gcagtgcggc agtttgatgc cattaacagc
    12961 ggttttacct atgatagtgc tgctcaaacc ctttcgcgcc gtgcccggaa actactggag
    13021 cacggggcac ccctgaactg ggatgggatt ttgccacttg ggaaaaaata gcaaaggaat
    13081 accgtgacag cagtacgaga gggtatgatg gtttctgtgc tgcgctctct cggcgcgttt
    13141 gcagcatttc tggccttcta tatttttgcc ggcgtttttg ttctgcgtta cctgacagaa
    13201 aatggtatcg tcgcggtttt ttggcctcct acgggcattg cactggcagc actgctactc
    13261 ggcggccggc ggtatatacc agtcgtattt atcggtgctt tctttgtgaa ccagttggcc
    13321 ggtgctcgta tcgttccggg tttaatctat gcgggcatca atacccttga gggcctcgtt
    13381 gcatggtttt tgctaacccg cgtgagcaag atcgatcttg cgcttgccca tgctcgcgac
    13441 tacttccaga tttttctcta cggtggtgtc gtcgcaccct tcgtcggggc gctgctcggg
    13501 gccataaccc tctacctgtc tgcactggcg ccgcaggatt tctggcacaa cctgcagtac
    13561 tggtggatgg gtgactcatt gggagttatc attcttacgc cgcttctgct gatctggcgc
    13621 aggcgcccca cacagacgta tacgagcagg cagtggatag aagctgcggt cggcctctct
    13681 gcccttgtct ttgttggcca gatcgtgttc gctggttggt cattcggcgc gctcaatgcc
    13741 tacggccgcg gtttcatttt tttcatcttc gtctcgtggg cagccatgcg tttcggccgc
    13801 cacgccactt cgcttgccat cgctatattg attatccaga ttatggtggg cctggtcgcc
    13861 ggccggggtt acttcgctgc agagagtcgc agcgtcatgt tcgggacgat atggctgtac
    13921 atgacggcaa tctcggtgac cggtatggcg ctggcgattt atatctacga ggcgcgcctg
    13981 ctcaatcacc aactgaccct cgcgcgtgaa cacctggagc aggtcaatgc tctggcgcat
    14041 atcggaggct gggagtttga tatagcggaa aacagcatgc gcttttcgcg cgaggcactg
    14101 cgcattctgg atttgccaga agatgctgac cccgatctgg caactgcgct gagtttcata
    14161 gatgagagtg aacaagccgt acacatggca cgcgggcagc tggcaatgga acaaggtatc
    14221 ggctgggatg ctgagttcgc gctgcgcacg cgttcgggca gacagatctg ggtgcggtcg
    14281 cagggcaggc caatcagcga aaatggccgc gtcgtgcgcc tcatcggagc gctgcatgac
    14341 ctcaccgaaa gcaggtatgc acagatagcg ctcaaaaagc gcgagctgga atttcgccgc
    14401 ctggtagaga ctgcctctga gggaatctgg acgattgatg agcatggctc gaccactttc
    14461 gtgaaccggc gcatggcaga attactgggt tatcagcccc acgaaatgct gggcacgcat
    14521 tttctcgaat acattcctgc tgaatggcat ggcgttgcca cacagcagtt gcagcgcaga
    14581 agagaagggg tttctgaaag tcaggagtct gtgttgcttc accgttcagg caagaagatc
    14641 tgggtcatgg cgagcaccag cccgattcgc gacgaagcag gcgtagtcgt gggagctctc
    14701 gcaatgatca gtgacatcag cgagcgcaag acaattgaag aacaactgaa acagagcgaa
    14761 gcgcgttaca ggcaactcgt cgaatcttcg gccgatgcaa ttgttgtgca ccaaagcggc
    14821 ttcatcgttt atttgaatga accggctgtg cgtctgctca aggcgcacga taccggtgac
    14881 cttattggcc gaaacgtgct cgagcttgtg gcgcctgaat ttcggaccac ggtgatcgag
    14941 cgtatgaaac tggtgaatgg accgggtggg cacgtgccgc ttgttgaaga gaaattcctt
    15001 cgcctggacg gtacgacggt agacgtcgag gtgtctgctt ctggcacgct cttcaatgga
    15061 ttgcctgcaa ccatggttgt tgcgcgcgac atcacggacc gcaagcgtgc cgaagcacag
    15121 atacgctacc tgggccagca cgacatactg actggtttac ccaaccgcgc cctgttcgcg
    15181 gaccgtctgg cacagacgat agcaatggca gatcggcatc acggagtttt cgccctgttg
    15241 tttctcgatc tcgaccactt caagaaaatc aatgattctt acgggcaccg ggctggggac
    15301 gcgttcttac ggcaggttgc agagcgtctc accggatgcg tgcgggccat caacacggtt
    15361 tcgcgccagg gtggagacga attcattgtg cttctcaatg acctggcgca ccctgaagat
    15421 gcaggtacaa ttgcccgtga aatttgtgaa cgtctttctg aacctttcga catcggggaa
    15481 atacgtctgc atgcttcagt cagtgtgggt attgcggttt atccgaaaga cggcagcgat
    15541 gcagacacgc tgctgcgcaa tgccgatatt gcgatgtacc atgcgaaggg cagaggccgt
    15601 aaccagtacc agttcttttc ggaagaactg aacaggatta cgcagcagcg actcgatatt
    15661 gagctggcgc ttcgcagagc gattgccgag aaccagtttt atctcgtgta ccagccacag
    15721 cttaacctgc agaccggagc gatcgaaggt tttgaagcgc tcatccgttg gcgacacccg
    15781 gtgcgcggag aactttcacc gctcgaattc atacccattg ccgaagaatc cggtcagatt
    15841 gccgcgcttg gtgaatgggt gctgcgcaag gtcttgtcag acatgccaca gttcgatgca
    15901 gcggggcatg accagctgcg cattgcggta aacgtcagcg cactcgagtt acggcagacg
    15961 aattttgctg atcgggttct taagttactt gaagaatttc acgtactacc gatgcgcctt
    16021 gagttagagg tcacggagag catgctcatg gaagacacgc agctagcggc gcagtcgata
    16081 caaaggttgt ctagcgaggg tattaaattc gccattgatg attttggtac aggctattca
    16141 tccctaagct acctgaggac gctccgcatt caccacctga aaatcgaccg ttctttcatc
    16201 agcgacctgg cacacgatgc agacgacgct gcgattgtca gaacgatcat cgccttggcg
    16261 cagaatctgc gcctgaaggc ggttgccgaa ggcgtcgaga cccaggaaca gcgggatttt
    16321 cttcgcaatc atggctgcga aatcatgcag ggcttctacc tttcccgacc gcttgagcta
    16381 cccgccgccc tgaagttcct ttctgacagt gtgcggtagt cacgtttcct caggatacgc
    16441 ctctatacca tgctcaataa tgtcgagacc ttcgatttct tcagaggccg gtactctcaa
    16501 gcctatgaac gggatgtatt tcatgagcgt gaataccgcc agtgaaaccg ggaatgccca
    16561 caagaaacca gcggcaacgc ccagcgcctg ggcggccagc tgtgccgtgc cgccgccata
    16621 gaacagacct gcaggtcctg cgccatagtt ggggttcgca aataatccca cagctaaagt
    16681 accccaggct ccggcgaagc cgtgtactga aactgcaccg acgggatcgt caatcttgag
    16741 ccagtcaagt agcaagacgc cgtagacaac cagtacccct gccacacctc ctatcgccac
    16801 agcccctttg ggatcgacgt tgctacagcc ggctgtgatt gccacgaggc cacccagaat
    16861 gccgtttacg accatactca cgtcgggccg tttaaagaga atccacgatg tgagaatggc
    16921 ggctacgccg ccagcgcaag ctgccaggtt agtcgttata gcgataattg cgaaagaacc
    16981 gtctttgatg gttccggttg aacccgggtt gaagccaaac cagccaaacc agagaatgaa
    17041 catgcccaaa acggacatgc tcatgttgtg gccaagaatt ggtttaactt catggccggt
    17101 gccgaacttg ccgatgcgcg ggccaagcgc gatggtgcct gcaagtccca tccagccgcc
    17161 gagagaatgg acgacggttg aaccggcaaa gtcgatgaaa ttctttgagg cgagccagcc
    17221 cacgttcttg ttgtcgaaga ggtttgacca gacaagacta ccgaacaggg gatagataat
    17281 agcactgcag attaaggaga agaatacata cgaaatgaat ttcgtccgct cggccatggc
    17341 gcctgatacg atggttgctg cagtgccggc aaaaaccatc tggaaaaaaa ggaatgcata
    17401 gttaaaggca atgggtttgc cgtccgggcc ttcgagcagc ctgtcaaaaa gtacaggcgt
    17461 tccgaagcca aagccagcga ccagctgtgg cccgaacatg agcgaaaacc cgatcgtcca
    17521 aaaccccagg gatccgacga gaaaatcgga ggtgttcttc atgaggatgt ttacgacgtt
    17581 cttctttcga gaaaatccgg tttctacata cgcaaaaccc gcctgcatga aaaagaccag
    17641 aaaacctgcg accatgagcc aaagcaggtc ggtgtcgctg cgtagatttt tgactgcctc
    17701 ttcagcattg aatgcggcgg cctgattttc ctgcgcaaaa attctttcac ctgaaaccag
    17761 cgtgaaaaat aagaccgcgc aggcgcgaag ggggtaatac ttcagtcggt tcatagagct
    17821 ctccagcacc tttcgacagc aatatgcatg ccaatttttc tgccagggcc taagccgccg
    17881 cttatatcgc ggtttacgct gcatcttgta tttgatgaat taccgcaggt gctgccatac
    17941 acaatggcag cgccatgaag aggaagtccg gtgagaatcc ggcgctgacc cgcaaccgtg
    18001 tttgcgcttt taggggtagg tttaaaccta cccctaaaag tgcataagtc gggaactctt
    18061 gtgtgtatcg cccgaggaca cggcgttaca ccgaaatgat tcgagtaaat tttttcctgc
    18121 tcgaatcgtg cgggctccgg agcagattgc gggagccatg ccggtacctc cggttcggtt
    18181 caaccccagt tgtgctcctc gggagcaact tatgaagaac aatagatttg cgtcaggcgt
    18241 atcactccgc ctggcaatgg tggcctttgc cgttgtgcta ttctgcgctg gctgtgccaa
    18301 tggcggcatg gcggta
//
LOCUS       DAOQJF010000014        17988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1465_length_17988_cov_5.591312, whole genome shotgun sequence.
ACCESSION   DAOQJF010000014 DAOQJF010000000
VERSION     DAOQJF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 17988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17988
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_17988_cov_5.591312"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLF85_01480"
     CDS             <1..307
                     /locus_tag="PLF85_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNE18354.1"
                     /translation="ISGSHRGGMVITCDKDLIAGLLQFVLGAEAESEEDIINMIAEMA
                     NTVSGNARVHFGSGFDISVPTVVVGEPEEFKFVLAEPTLVIPFTWLGHRANAIIGLT"
     gene            complement(377..700)
                     /locus_tag="PLF85_01485"
     CDS             complement(377..700)
                     /locus_tag="PLF85_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18355.1"
                     /translation="MEENVNKVVSFGIGAFESARASAETLLKNLETEINALIASGESV
                     QSENAVKVRSAVTNASQRVSTLVEQAKAQYSDLSVKAQKALADLQAKASELAAKLPGS
                     KQQAA"
     gene            complement(800..1324)
                     /locus_tag="PLF85_01490"
     CDS             complement(800..1324)
                     /locus_tag="PLF85_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE18356.1"
                     /translation="MWLIEADKALFEYLTLYCNPPWVSALSETIGNLSTYTWPFVIFC
                     VLYYWLNSPRFVRFLLAVSVLLIFSEGSAYLLKQLVARPRPAVEWLIYVDPKAVGFPS
                     AHSVNTMALAFFLSRWFKIPLAWYLPLPLVMGASRVLANYHYPLDVAGGWLLGFTVAW
                     LYWRLVQHWRWLMQ"
     gene            1418..2797
                     /gene="lpdA"
                     /locus_tag="PLF85_01495"
     CDS             1418..2797
                     /gene="lpdA"
                     /locus_tag="PLF85_01495"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNE18357.1"
                     /translation="MASSFDYVVIGAGPGGYSSAIRAAQLGLKTAVIEKENAGGVCLN
                     WGCIPTKALLTSAHLLLKLREGKLGVTAKDGFSADLPAIVDRSRAVAARLETGVKSLF
                     KKYGVTMIQGTATFISPTEISVSGAEKQQIRFKQACIATGASARPLPQLKFSPRVWGY
                     RDALVQKTLPKKLTVVGGGAIGLEFADFYHCLGTEVTLLEMAPHILPNDDDKVAAALR
                     RALEQRGMQILEGAKILTAAEAAGGVSLDIEYNGEKKNLTSDSALIAIGVTPNTSGIG
                     IDKFGIVDERGFVRVDANQQTTTKGIFAIGDATAGKLLAHRAAHQGIFVAEFIAGKHP
                     HPVGEIPGCIYTSPQVATVGRSEAELKQAKREFKTGEFPWLASGMALAAEEKNGFSRV
                     FTDAKTGEILGAQIVGEHAAELIGQVSLAMGSELLADDFLRAVMPHPTLSETVFQAFA
                     VLEGVAVDY"
     gene            2845..3456
                     /locus_tag="PLF85_01500"
     CDS             2845..3456
                     /locus_tag="PLF85_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18358.1"
                     /translation="MRLPCSIGAPFLFVLAVGALSSAEKQVEPLRVIAERSCKCIEEI
                     DFSKLKSSEQRNAQLGLCVIKSAKGFEKFLLTEKNIDMDRIGESDHGTRLGYLFGVEA
                     ATVCPETLARVTSETESAQSTVAGRVTETQTGAYTRLTVEDMAGRTTTLYWIFNFDNS
                     NLLLKSLADKTARYEFSFAEKEVFFLARKEYQKVKVITGLKKI"
     gene            3507..4151
                     /locus_tag="PLF85_01505"
     CDS             3507..4151
                     /locus_tag="PLF85_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNE18359.1"
                     /translation="MKKIRITLGCVAAMSAFALLAQNSSTPERRTNVTLPQSAPGMPA
                     APAKSSACMTPEEVRLVDAINNYRRSKGLPAVQPGVSLTRVAQLHAADLTQNQPARGA
                     CNMHSWSSNGNWTPCCYTSDHRQAACMWKKPAELTSYKSNGFEISAGSSGGRIDAATA
                     LRVWQGSSGHNAVIINAGMWRQPWGTFGVGIYGGYAVAWFGQQADSAGAAIPCK"
     gene            4187..5110
                     /locus_tag="PLF85_01510"
     CDS             4187..5110
                     /locus_tag="PLF85_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE18360.1"
                     /translation="MAQETAESTLRLAVAKTKKLYIGGEFPRSESGRSFPLTAKQTGK
                     FYANIAHASRKDARNAVEVARKAFSSWSRKTAFNRGQILYRMAEMLEARSAEISSELS
                     VVSGVTAAAAKREVEASIDRLVWYSGWCDKFQQLSGNHNPVSGSYFDFTIPEPMGVVV
                     AACPNYAPVLGFISQVAPIIAGGNSVIAISSEENPAVVVSLAEVLATSDLTGGVVNVL
                     TGFRKELLPGLAQHMDVNALDLAGITDAERIEVEKAATENLKRIRIKSWKSADDLYGS
                     VGEKLDYITDFQEYKTIWHPIEAFGGGSGKY"
     gene            complement(5096..5830)
                     /locus_tag="PLF85_01515"
     CDS             complement(5096..5830)
                     /locus_tag="PLF85_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18361.1"
                     /translation="MHRGILEIVFCAGVHGSADNIRQWVQMYRQLAADPLPNNLAEFQ
                     ASLDQQVAGIEKFADVELTDDLVQKEVMRLFDAGATEVILPYSELRWRNHAEWVFHKR
                     NSEQFFVSNNMQQSPLQYIRSRVQNFCDSKGMSDSHRDEVVISVTEAAENAIKYSNVM
                     PVFIEHGIEKDEYFIRAINSVNDLNLKDEISRGKFSEDVSLMRGVLVMSKLLDYLDIV
                     RDMDKKRVEFVGRKKVSLKAPKLVLS"
     gene            5989..6783
                     /locus_tag="PLF85_01520"
     CDS             5989..6783
                     /locus_tag="PLF85_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE18362.1"
                     /translation="MRYLLIPVGFLFYLWFLVIFIPVTIFFAATVLVVSPFWPKKYGR
                     AMRFFPRAWGWIGIRLVLCPVYIYGAEKAQDNPGIVISNHQSLFDILAALGFYPMDFL
                     FLSKAEVFKIPLVGLAMRKVGYIPINRSNPRQAARSLEVVEAQVRENNRVLIYPEGTR
                     SKDARKLLPFKAGAFQVAEKGSFPIFPVVLYGTQETRPMHSNVLLFPHRIAIQVLDPI
                     MPDNELHPANSQSRLSEKEKMAGIRAVVEKAYLQLADSKGLKPAQL"
     gene            6791..7474
                     /locus_tag="PLF85_01525"
     CDS             6791..7474
                     /locus_tag="PLF85_01525"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HNE18363.1"
                     /translation="MRVARVLFARIPEPGAVKTRLAADLDETSAYAIYRWLLRIQKRL
                     YANPAPTGCTYTDYVYYAPRINRLTARLRFAPDLTGLRLKFRPQAEGDLGLRLARATA
                     EVLKSHDLALIWGADIPALPPSIFEQAIALYPQSVITLARDGGYAFVSIARQNFTPQI
                     FSGIRWSTHHTGRDQLRALLRAKVPVTVSGKVADLDRAKDFTRIIRELESFGRGPDLD
                     DLTVTLRNL"
     gene            7540..8739
                     /locus_tag="PLF85_01530"
     CDS             7540..8739
                     /locus_tag="PLF85_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNE18364.1"
                     /translation="MKMENGPLTGVKVLDLSLLLPGPLCSQHLADMGAEVIKVENPRL
                     GDMTRYLTTKVSKGDFAEAGAFLQLNRNKKSITVNIKREGGRDVIMRLLKDADILLEG
                     FRPDTLNELGIGYEQLKEKFPRLIYCAISGYGATGHYKEHAGHDANYIARSGLLALNG
                     VKGGPPVVPGFQVADIAGGTLTALAAILAALYAREKTGKGQFIDVSMQDGATPFLSLL
                     LGDYIATGKEPERGNDMLSGMLPNYKIYETKDGKYVMLAALEEKFFQTFLRQIEREDI
                     LKNMSRDEAGFTAATAELEKIFKSKTYAEWAPLFENPDSCFSGLSSLGEVLQDKHLRD
                     RGTVLEIDHPKLGKVPVIGSPFHFSETPVSYRLAPPEMGQHTEDVLKSAGFSAAEIEQ
                     LKKDKCV"
     gene            8747..9175
                     /locus_tag="PLF85_01535"
     CDS             8747..9175
                     /locus_tag="PLF85_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNE18365.1"
                     /translation="MYEKGKTFDAIQIGDTASFSKTITETDVYLFAGISGDFNPLHVN
                     EEYAKQSPFKTRIAHGGLAASLLANVLGMKLPGLGTVALEVTQKFRNPVYFGDTVTCA
                     VTVSKKIERMKIIEMQILWSNQRGETVSKGSCRVLPPNAD"
     gene            9303..9707
                     /locus_tag="PLF85_01540"
     CDS             9303..9707
                     /locus_tag="PLF85_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18366.1"
                     /translation="MKRTGLAIVAAIFVFTACGKANAVKDFGQLFDDGTKSLQSLGKD
                     LEEAKDGKAVAAGIDKFVDLIKSFKEKGEALEKKHGMSAKGEMPAELKVKAEAFQKAA
                     EELGKGPMAKAMMKYAAAPEVKAAMAKLQSLR"
     gene            9817..10716
                     /locus_tag="PLF85_01545"
     CDS             9817..10716
                     /locus_tag="PLF85_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE18367.1"
                     /translation="MQKPPALIIDPDLALQEILGDILKNRFRIFCANTIAESAEVMRG
                     EDPHLIFIEMSLPGESAIDYIERTKRSKTNTQFIVITDNATVENAVSALRQGALDFLK
                     KPFEIEDIARIVEKFFSLTANRDADYDIYSSIVAESRTFELPTDFAIISTFMSEIMHI
                     ISRFAGLDKKTTLTLRLAVYEMLVNAMEHGNLEIDYEMKKNLLEEVMDYQRFLNDRAN
                     QEPYRNRKVRVTYQYDRDAIAFTITDEGKGFDVSKVPNPHSSENLERLNGRGIFISRV
                     NMTRVTYNEKGNSVTLYKELPKA"
     gene            complement(10723..11634)
                     /gene="rsmH"
                     /locus_tag="PLF85_01550"
     CDS             complement(10723..11634)
                     /gene="rsmH"
                     /locus_tag="PLF85_01550"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNE18368.1"
                     /translation="MLRDAVVRAAECLSALPPGQRKVWDGTTGLGGHLLAISEQYPDA
                     RLLASDADAAMLELARERVQNRVAVYRHANFAEIPFADEAPLGFIFLDLGISSAHFDY
                     FERGFSFRFDQPLDMRMDTSAGISAAELLQRESEEGLARIFFEYGDEKLSRRIARAIV
                     ARRRTKPVMTTTQLAEICKAIYPPKYKAKGHADRHPATRVFQALRIAVNDELGVLRTA
                     LEMLPRQLAPGGRMAVISFHSLEDRLVKHAFRNLAYIPETNPQAKSNFRDGDFRLIEP
                     GGIAPDEEEIRRNPRARSARLRILERR"
     gene            complement(11664..14264)
                     /locus_tag="PLF85_01555"
     CDS             complement(11664..14264)
                     /locus_tag="PLF85_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNE18369.1"
                     /translation="MFDFTNRVKKIINEYAPREAKRLGHEFLGPEHILLGLLKAQDSV
                     AVKMLMNLGIDLNELKKEVEKRSEQDSATMVIDPTSKDKVQRILELSRDEARKMRHSY
                     IGSEHVLLALLRDTNGVVASSLQTFQVNYQVIRNELNATLGLPQATSTQKTQSSAAQP
                     KKEASKTPMLDEFARNLSRMAEEKLTDPVIGRDHEIERVIQILSRKTKNNPILIGEAG
                     VGKTAIAEGLAQRIAQKRVPEPMFNRQVYSLDVAALIAGTKYRGEFEDRIKKIMKEIR
                     TNKEVILFIDEVHTIIGAGAAEGAVDAANILKPALARGEIQCIGATTLREYKRYIERD
                     SALERRFQSVMVEEPSVEDAIRILAGLRHAYEKHHAVSFSKEAIVAAVKLSDRYIKDR
                     FLPDKAIDIIDEAGSHARLKSAVIPEEVKEIEKEIQQLTRDKNEMVRQQEYEKAAKLR
                     DQIGEANARLADAMNSWRRSQKEARVEITAEDISKVIADWTGIPLQQLNESENERLIK
                     MADELADRVVGQKTAIDQISRAVRRARMGLKSVRKPIGSFFFLGPTGVGKTELAKALA
                     EFMFGEEEALIRFDMSEYMEMHSVSKFIGSPPGYVGHEDGGQLTEAIRRHPHSVLLFD
                     EIEKAHPEIQNILLQVLDEGELTDNAGHHVDFKETIIILTSNIGARFLQKGGKLGFAG
                     ETENKQEQKREQVLEELRKHFSPEFLNRIDDVIIFDPLTKEEIQEIVELTIDEINYNA
                     LVKNMFLTLKDDARSYLADKGYSEKYGARPLKRLVQREIEDELAMMLLEKKITEPVEI
                     NIGLTEKDGNKKLDFTFKNLSAEKFLEIKGEYFEDEATIDEIWENSLLGSEEGEDEAE
                     PQAEPEVTQK"
     gene            14391..14723
                     /locus_tag="PLF85_01560"
     CDS             14391..14723
                     /locus_tag="PLF85_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18370.1"
                     /translation="MKSLFKRRTKKHDDSIAGTERPWESCLAVCSKCARKVKAMDGDK
                     TKLRVGLKALVSLRGLKKKLRPVDITCLDVCPEDKIVVAHMTPGGVTLKIVGPDAKPE
                     QVLSEFGF"
     gene            complement(14766..15251)
                     /locus_tag="PLF85_01565"
     CDS             complement(14766..15251)
                     /locus_tag="PLF85_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE18371.1"
                     /translation="MNAHRHCHYCGSQYPQVESFPRTCPACGRQVWSNPLPVAVVAVR
                     AEGGVLIIKRGIEPAKGKWALPGGFLETGETWQEGSCREVFEETGLRIDPAGIELVEA
                     LSVAQNSQIILFSVTRSSYRLSDFAFESSEEALDLRVMREPEELAFAGHTTFARKFLY
                     L"
     gene            15311..15751
                     /locus_tag="PLF85_01570"
     CDS             15311..15751
                     /locus_tag="PLF85_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18372.1"
                     /translation="MKLSTWFPASTEGQKMALRTFIILLFLLCFASCKKKAPLDYFPV
                     EERGGNIVMREFARDSEKSSHYVLMNGAERPHFHDRHSFIVRLVSGRNRLHLVSATHE
                     LAPGETMRIEKGSLHWAENAGPGFSILQVDFYPPFDGTDRRFAE"
     gene            15755..16819
                     /locus_tag="PLF85_01575"
     CDS             15755..16819
                     /locus_tag="PLF85_01575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE18373.1"
                     /translation="MVAVGQWGEGSSLGSAFSSAWEGTKTLANQAWSGVTNTFERTVN
                     LVAGNGWHNKAEYEALKLAQLSGGWEASRALVTGDLSSPGLEGDLRFNFGMGGEAQMG
                     QALLAQMRQNGGGTQAILQKFILNSRGEAIKDLQALGVKVSYDKKTKQLVIDQNQSDD
                     LKKLAAELNLAMKTWDGNVANGMASENTRLYNQTWSAKFSQIHETSSGARIPYGNEFV
                     GDQYGWRDLDSDPATGKDGKEDFHYGTDFRVGAGKSIPALASGRVIELELNHKTYGKT
                     ILIDRGNGLYSRIAHASELAVRQNDSVTTNQVLGLTGSTGGKSLGPHLHLMLYTKDEK
                     NNLKAVDVLKVDWRKYGRPR"
     gene            complement(17047..17517)
                     /locus_tag="PLF85_01580"
     CDS             complement(17047..17517)
                     /locus_tag="PLF85_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18374.1"
                     /translation="MPATGWATPLQHDVAADNFTPDARRKIIQLRKNQHGAQQCLTRR
                     GAAMKRTLSVVEGSRRVKHVGRPVGFAEGKRAGAREGIQFNLRVNLVWPVKARVLSSL
                     EFSISFDEFKSANLLTAIFLPDFDLWRFNHPKTFIRDNLQRAYLFASIENVTVH"
BASE COUNT         4137 a         4860 c         4892 g         4099 t
ORIGIN      
        1 tatctctggt agccaccgcg gaggcatggt aatcacctgc gacaaggacc tgatcgcagg
       61 cctgctgcag tttgtactcg gagccgaagc tgagtctgaa gaagacatca tcaacatgat
      121 cgccgaaatg gcgaataccg tttctggcaa tgcgcgcgtg catttcggca gcggctttga
      181 catttcggta cccactgtcg ttgtcggcga acccgaagaa tttaaatttg tgctcgcaga
      241 gccgactctt gtcattcctt tcacctggct cggccaccgc gccaacgcga ttatcggctt
      301 aacataaaaa aaagcgcctt gcggcgcttt gggtagtgca atctaaatgg cgcctgagcg
      361 accattcaaa tgactattaa gcagcttgct gctttgaacc tggaagctta gcagcgagtt
      421 cgctggcttt tgcctgcagg tcagcgagag ctttctgtgc ttttacgctc aggtcgctgt
      481 actgtgcttt cgcctgttca acgagagttg aaacacgctg tgaagcgttg gtcactgcgc
      541 tgcgtacttt gactgcgttt tcagattgta ctgattcgcc tgaagcgatc agcgcgttaa
      601 tttctgtttc gaggttcttc agcagggttt ctgcgctggc acgtgctgat tcaaaagcac
      661 cgataccgaa gctaactact ttatttacat tttcttccat tgtatgctcc ttcccattcc
      721 cttggggcag cgcactttgc agtgcgccgt ctctacgcac aagctttgtg cgctgcacat
      781 agtgtcaagc ataaatcggt tattgcatca gccagcgcca gtgctgcacc agtcgccagt
      841 aaagccacgc aaccgtgaag cccagcaacc agccacccgc gacatcgagc gggtagtggt
      901 agttggctag aacgcgcgac gcccccatga ccagtggcag cggcaggtac catgccagcg
      961 ggatcttaaa ccagcgcgag agaaaaaatg ccagcgccat cgtgttcacc gagtgcgcag
     1021 agggaaatcc cactgctttg gggtcgacgt agatcagcca ttcgacggcg ggccgcggtc
     1081 gcgcgaccag ctgcttgagc aggtacgcac tcccctcgct gaagatgagc aacacgctca
     1141 ccgcgagcaa gaaccttaca aatcgcgggc tgtttagcca gtaatacagg acacagaaga
     1201 tgacgaaagg ccaggtgtag gtcgagagat tgccgattgt ttcgctgagc gcactcaccc
     1261 aggggggatt gcagtagagc gtcaggtatt caaacagtgc cttgtctgct tcgatcagcc
     1321 acacgagaca ggctcgcgcg cttcttcgca gcggcaacac aaacgctgtt aagccaaaag
     1381 cacttgtcca cagagcttgg tttattcttt ttgcggcatg gcttcttcgt tcgactacgt
     1441 ggtgatcggc gcgggcccgg gtggctatag ctctgcaata cgtgccgcgc agctcggcct
     1501 caagactgcc gtgatcgaaa aagaaaacgc cgggggagtt tgcctgaact ggggctgcat
     1561 acccacgaag gcattgctga cctcggcgca cttgctgctc aaattgcgcg agggtaaact
     1621 cggcgtcaca gccaaagacg gattttccgc agacctgccg gcaatcgtcg accgctcgcg
     1681 cgcagtggcc gcgcgcctcg agacgggtgt aaaatcgctc ttcaaaaaat acggcgtgac
     1741 aatgatccag ggcacagcga ccttcatttc accgactgaa atttctgtct caggcgcaga
     1801 aaaacagcag atccggttca aacaggcctg catcgcgacc ggtgccagcg cccggccatt
     1861 gccgcaactg aaattctccc cgcgagtgtg gggctaccgc gacgcgctgg tgcaaaagac
     1921 cttgccgaaa aaactgaccg tcgttggcgg cggtgccatc ggcctcgaat tcgcagactt
     1981 ctatcactgc ctcggtaccg aggtcacact gctcgaaatg gcgccgcaca ttctgcccaa
     2041 cgacgacgac aaggtggccg ctgcgctgcg ccgcgcgctc gaacagcgtg gcatgcagat
     2101 actcgaaggg gcaaaaattc tcacagccgc agaagccgcc gggggagttt cactcgatat
     2161 cgagtacaat ggcgaaaaga agaatctcac ctcagactct gcacttatcg ccatcggggt
     2221 tactcccaat acttctggca tcggcattga taagtttggc atcgtcgacg aacgcggttt
     2281 cgtgcgcgtt gacgcgaacc agcagaccac gacaaagggc atctttgcta tcggcgatgc
     2341 cacggcaggt aaactactcg cgcaccgcgc tgcgcaccag ggcattttcg tggcggagtt
     2401 catcgccggc aagcacccgc atcccgtggg tgaaataccc ggctgcatct acacatcacc
     2461 gcaggtggcg accgttggcc gcagcgaagc ggaactcaag caggcgaagc gtgaatttaa
     2521 gaccggtgag tttccctggc tggcatcggg catggcgctg gcggcagaag aaaagaatgg
     2581 tttcagccgc gtctttaccg atgcaaagac cggcgaaata ctcggcgccc agattgtggg
     2641 cgagcacgcg gccgaactaa ttggccaggt ttcgctcgcg atgggctctg aactactggc
     2701 cgatgacttt ctgcgcgcgg tgatgcctca cccgacgctg tctgagacgg tcttccaggc
     2761 gtttgctgta ctcgaagggg tggcggtaga ttattgaccg gagtcctcgc ttgacgccag
     2821 aaaacttctt tgtctagctg tgctatgcga ttaccctgta gcataggcgc cccgttcctg
     2881 ttcgtccttg cggttggtgc cctgtccagt gcagaaaagc aggtagaacc cctgcgtgtc
     2941 attgctgaaa gatcatgcaa gtgcattgag gaaattgatt tttctaaact taaaagcagt
     3001 gagcagcgca acgcgcagct gggcctgtgc gtcatcaaaa gcgcaaaagg gtttgagaag
     3061 ttcctgctga cagaaaaaaa tatcgatatg gatcgaatcg gcgagagcga tcacggcaca
     3121 aggctgggtt acctgttcgg tgtcgaggca gccactgtct gccccgaaac gctggcgcgg
     3181 gttacaagcg aaaccgaatc agcgcagagt actgttgccg ggcgcgtgac cgaaacgcag
     3241 accggcgctt atacgcggct gactgtcgaa gacatggctg gcagaacgac cacgctgtac
     3301 tggattttca acttcgataa ttcgaatctt ctgctcaaaa gcctcgcaga caaaactgcc
     3361 cgctatgagt ttagctttgc agaaaaggaa gtttttttcc tggcgcgtaa agagtatcaa
     3421 aaagtgaagg tcattacggg gctaaaaaag atctgatcgg catcgcctgc agtaaaacaa
     3481 gaactggatc aaagcaccgg agatatatga aaaagataag aatcaccctg ggctgcgttg
     3541 cagccatgtc agccttcgcg ctgcttgcgc agaatagttc aactccagag cgcaggacca
     3601 atgtaacgct gccgcaaagt gcaccgggca tgccggcagc accggcgaaa agttctgcct
     3661 gcatgacacc cgaagaggtg aggctcgtcg atgcgatcaa caattaccgg cgcagcaaag
     3721 gcctgcccgc tgtacagccc ggtgtttcgc tgacgcgcgt cgcgcagcta catgcagccg
     3781 atctcacgca gaaccaaccg gcgcgcggcg cctgcaatat gcacagctgg tcatcgaacg
     3841 gcaactggac tccctgttgc tatacctctg atcaccgcca ggccgcgtgc atgtggaaaa
     3901 aaccggccga gctaacctcg tataaatcca atggctttga aatctctgcc gggtcgtcag
     3961 gcgggagaat cgatgcagct acggcgctgc gggtctggca ggggtcgagc ggtcacaatg
     4021 ccgtgattat caatgccggt atgtggcggc aaccctgggg gacgttcggc gtcggtatct
     4081 acggcggtta tgccgttgcc tggttcgggc agcaggcgga cagcgcgggt gcggcaatac
     4141 cctgcaaatg atttgacacc gctggctaac gcgaaactcc ctttctatgg cacaggaaac
     4201 agcagaatcg accctccgcc tcgctgtggc gaagacgaaa aaactctata tcggtgggga
     4261 gttcccccgt tctgaatcag ggcgctcgtt tccactgacc gccaaacaga cgggaaaatt
     4321 ttacgcaaac attgcacacg ccagccgcaa ggatgcacgc aacgcggttg aagttgcgcg
     4381 caaggccttc agcagctgga gcagaaaaac cgctttcaac cgcggccaga ttctgtaccg
     4441 tatggcagaa atgcttgaag cgcgttctgc tgaaatcagc agcgaactga gcgtcgtctc
     4501 cggcgtaaca gctgcggcgg caaaacgtga agtcgaagcc tcgatcgatc gtcttgtctg
     4561 gtattctggc tggtgcgata aattccagca gctttcgggc aaccataacc cggtgtcagg
     4621 ctcatacttc gacttcacca tacccgaacc gatgggtgtg gtcgtggcag cttgcccgaa
     4681 ctatgcaccg gtgctcggtt ttatttcaca ggtcgccccc attattgccg ggggaaattc
     4741 tgtcattgcc atcagctcag aagagaatcc tgccgtggtc gtgagcctgg ccgaggtgct
     4801 cgcgacatct gacctgacgg gtggggtggt gaacgtcctg accggttttc gcaaagaatt
     4861 actgccgggt ctcgcgcagc acatggatgt taacgcactc gaccttgcag gtattaccga
     4921 tgccgagcgc attgaagtgg aaaaagcggc gaccgagaac ctgaagcgca tacgcatcaa
     4981 atcgtggaaa tctgccgatg acctctatgg cagtgtaggt gaaaaactgg attatatcac
     5041 tgacttccag gaatacaaga caatctggca ccccatcgag gcgttcggtg gcggctcagg
     5101 aaagtactag tttcggcgct ttcagagaaa ctttcttgcg gccgacgaat tcgacccgtt
     5161 ttttgtccat gtcgcgcacg atgtcgaggt aatcgagcag cttcgacatc accagtacgc
     5221 cgcgcatcag cgagacgtct tcggagaact tgccgcgtga aatttcatct ttcaggttaa
     5281 ggtcattgac cgagttaatt gcgcggataa aatactcatc tttctcaatg ccgtgctcga
     5341 tgaacacggg catcacgttg gagtacttga tggcgttttc agcggcctca gtgaccgata
     5401 tgacaacttc gtctcggtgc gaatcgctca tccctttgct gtcgcagaaa ttctgtaccc
     5461 tggacctgat gtactgcagc ggcgactgct gcatgttgtt ggaaacaaag aactgttcag
     5521 aattgcgctt atgaaaaacc cattcagcat ggttgcgcca gcgcagctcg ctgtatggga
     5581 gaataacttc tgttgcgcct gcatcgaaaa ggcgcatgac ctctttctgc accaggtcgt
     5641 ctgtcaattc gacgtcggca aatttctcaa tacccgcgac ctgctggtcc agggacgcct
     5701 gaaattcggc aaggttattc ggcaggggat cggcggcgag ctgtcggtac atctgcaccc
     5761 attgccggat attatccgcg ctgccatgaa ccccggcaca gaatacgatc tctagtattc
     5821 cgcgatgcat ctaccgctaa attacgcatg atgcctcggg gtcaagaaat aaatgatcat
     5881 tcgtgtcatg cggagcgggc tctttacttc gctgccacgg taagtaaaaa gcaccaggcc
     5941 gcgttcaata accatttgac gtgtcgttgc caaaaggtag cgccccccat gcggtacttg
     6001 ctgatacccg tcgggtttct cttctacctg tggttcctgg taatttttat tcccgttact
     6061 atattctttg ctgccacggt tctggtcgtt tcgccgttct ggcccaaaaa atacggccgc
     6121 gcgatgcgtt tttttccccg cgcctgggga tggatcggta ttcgactggt actctgcccg
     6181 gtatatatct atggtgccga gaaagcacag gacaaccccg gtatcgtcat cagcaaccac
     6241 cagtcgctgt ttgatattct tgcagcgctg ggtttttacc cgatggattt tctttttctt
     6301 tcgaaggctg aagttttcaa aatcccgctc gtcgggcttg ctatgcgcaa agtcggttat
     6361 attcccatca accgctcaaa cccacggcag gctgcgcgct ccctcgaagt ggttgaagcc
     6421 caggtgcgcg agaataaccg cgtgctgatt taccccgagg gcacccgcag caaagatgcc
     6481 cgcaagctgc tgccgttcaa ggccggcgca ttccaggtcg ccgaaaaggg cagcttcccg
     6541 atttttccgg tcgtgctcta tggcacgcag gaaacacgcc cgatgcacag caacgtgctg
     6601 ctgtttcccc accgtatcgc gatccaggtg ctcgacccga tcatgcccga taacgagctg
     6661 catcccgcga acagccagag ccgcctgagc gaaaaagaga agatggcagg catcagggca
     6721 gtcgtcgaaa aagcgtacct gcagctcgcg gacagcaaag gtctcaagcc cgcgcagctc
     6781 tgagctgcgc atgcgtgttg cccgcgtcct ctttgcgcgt atacctgaac ctggcgcggt
     6841 caagacacga ctggctgccg acctcgacga aacatcggcc tacgcaatct accgctggtt
     6901 actgagaata cagaagcggc tctatgcaaa tcccgcacca accgggtgca cctataccga
     6961 ctatgtctac tacgcgccgc gcatcaaccg ccttaccgcg cgcctgcgtt ttgcacccga
     7021 ccttaccggc ttgaggctga agttccggcc gcaggcagaa ggtgatcttg gcctgcgcct
     7081 ggcaagagcc actgctgagg tactgaagag ccacgacctc gcgctcatct ggggcgcaga
     7141 cattccggct ctgccgcctt ctatcttcga gcaggcgatt gcgctctacc cgcagagcgt
     7201 catcacgctc gcccgtgacg gtggttatgc tttcgtttct atcgcgcggc aaaatttcac
     7261 cccgcagatt ttttccggta ttcgctggag cacgcaccat acaggccgcg accagctgcg
     7321 ggcgctgtta cgggcaaagg taccggtgac agtttctggc aaggtggcgg atctcgaccg
     7381 tgccaaagac ttcacgcgta ttatcaggga gctcgaatcg ttcggccgcg gccccgatct
     7441 ggatgatctc accgtgacct tacgcaatct gtaaactctg tagcgttcgc tacagaagaa
     7501 atcgctttgc agaacgtgcg caaaaaaaac tcctgaggca tgaaaatgga aaacgggcca
     7561 ttgaccggcg ttaaggttct cgatctttcg ttactgctgc cggggccatt gtgttcgcag
     7621 cacctcgccg acatgggcgc tgaagtcatc aaagtggaaa atccccggct gggtgatatg
     7681 acgcgctacc tgaccaccaa ggtcagcaag ggtgactttg ccgaggctgg ggcatttctg
     7741 caactgaacc gtaacaaaaa atcgattacc gtcaatatta agcgcgaggg tggccgtgac
     7801 gtgatcatgc gcctgctcaa ggacgcagat attctgctcg agggcttcag gcccgatacg
     7861 ctcaacgagc tgggtattgg ctatgaacaa ctcaaagaaa aatttccccg cctgatctac
     7921 tgcgcgatct caggttacgg cgccacaggt cattacaagg aacatgcggg tcacgatgcc
     7981 aactacatcg cgcgttcagg cctgctcgcg ctgaacggtg tgaagggcgg cccaccggtc
     8041 gtcccgggtt tccaggttgc cgacattgcc ggcggtacgc tgaccgcgct cgcggcaata
     8101 ctggctgcac tctacgcgcg cgagaagacc ggcaagggcc agttcatcga cgtttcgatg
     8161 caggacggcg cgacaccttt cctgtcgttg ctgctcggcg actacatcgc cacgggcaaa
     8221 gaacccgaac gcggcaatga catgctctcg ggcatgttac ccaactacaa gatctacgaa
     8281 accaaagatg gtaagtacgt catgcttgcc gcgctcgaag agaaattctt ccagactttc
     8341 ctgcggcaga tcgaacgtga agatatcctg aaaaatatga gccgcgacga agcaggtttc
     8401 actgccgcaa cagcagaact ggaaaaaatc ttcaaatcga aaacatatgc tgagtgggcg
     8461 ccgctgttcg agaaccccga cagctgcttc tctggcctga gctcgctcgg cgaagtacta
     8521 caggataaac acctgcgcga ccgcggtacc gtactcgaga tcgatcaccc caagctgggt
     8581 aaagtacctg ttatcggttc gccgttccac ttttcggaga cgccggtcag ctaccgcctc
     8641 gctccgcctg aaatggggca gcataccgaa gacgtgctga agtctgcagg ctttagcgcg
     8701 gcagaaattg agcagctgaa aaaagacaag tgtgtctgag gtaaatatgt acgagaaagg
     8761 caaaacattc gacgcaatac agattggcga cacggcaagt ttcagtaaga cgattaccga
     8821 aacggacgtg tacctgttcg caggtatcag cggtgatttt aacccgctgc atgtcaatga
     8881 agagtacgcc aaacaatccc cctttaagac gcgtattgcg catggtggcc tggccgcttc
     8941 tctgctcgcc aatgtccttg ggatgaaact cccgggtctt ggtacggtcg cgctcgaggt
     9001 cacgcagaaa ttccgtaacc cggtctactt cggcgacacg gtaacctgcg ccgtgacagt
     9061 ttcaaagaaa attgagcgca tgaaaattat cgagatgcag attctttggt ctaaccagcg
     9121 cggcgaaacc gttagcaagg gaagttgccg ggtcctgccg cccaacgccg actaacgact
     9181 tgatatccgc ctgcacattt gttttgtctg ccgtctaagg gctgtcaaaa acaccctggc
     9241 cgctgctact gacttgcctg tgcgatgttg gttccagagg aatatttgat ttaggagaca
     9301 acatgaaaag aacaggtttg gcaattgttg ccgcgatctt cgtgttcaca gcatgcggca
     9361 aagccaatgc ggtgaaagat ttcggccaat tatttgacga tggtaccaag agccttcagt
     9421 cactcggtaa agatcttgaa gaagcaaaag acggtaaagc agtagccgcc ggcatcgaca
     9481 agttcgtcga cctcatcaag agcttcaaag aaaaaggcga agcacttgag aagaaacacg
     9541 gtatgagtgc aaaaggcgaa atgcctgcag aactcaaagt gaaggcagaa gctttccaga
     9601 aagccgctga agaactcggc aaaggcccaa tggcaaaagc gatgatgaaa tacgcagcgg
     9661 ctcctgaagt caaggctgct atggccaagc tgcagtcact gcgctgattt tcccttaaaa
     9721 tccagcctgt atttcggcac tatgcccgga atacaggctg atagttacct ttctcttgac
     9781 acgtaatctt ttcacccata tccgctgact acttcaatgc agaagccgcc ggcacttatc
     9841 attgatcccg acctggcgct acaggaaatc ctgggcgata ttctcaagaa ccgttttcgt
     9901 attttctgtg ctaataccat cgccgaaagc gccgaagtca tgcgcggcga agacccgcac
     9961 ctcatcttca tcgagatgag cctgccgggc gaaagcgcaa ttgactacat tgaaagaacc
    10021 aaacgcagca agaccaacac acagtttatc gtcatcaccg ataatgccac cgtcgagaac
    10081 gctgtcagcg cgttgcgcca gggagcgctc gactttctca agaaaccttt cgagatcgaa
    10141 gacattgcac gtattgtaga gaagttcttt tccctcacgg ctaaccgcga tgccgattac
    10201 gacatctatt cgagcatcgt ggcagagtcg cgtacattcg aactgccgac tgattttgcc
    10261 atcatatcga ccttcatgtc agagatcatg cacatcattt cgcgttttgc gggcctcgac
    10321 aaaaaaacca cccttacgct gcggctcgcg gtttacgaaa tgctcgtgaa cgccatggag
    10381 cacggcaatc ttgaaattga ctacgagatg aagaagaacc tgctcgaaga ggtaatggac
    10441 taccagcggt ttctcaatga ccgtgccaac caggagcctt accgcaaccg caaggttcgt
    10501 gtcacctacc agtacgaccg cgacgccata gcctttacga ttaccgacga gggaaaaggg
    10561 ttcgacgtca gcaaagtgcc caacccgcat tcgagcgaaa acctcgaacg tctcaatggc
    10621 cgcggtattt tcatttcacg ggttaacatg acgcgggtga cctacaatga gaaaggcaat
    10681 tcggtcacgc tctacaaaga actgccaaag gcataaggcg tattagcgcc gttccagtat
    10741 tcttagccgc gcggatcgcg cgcgcgggtt gcggcggatc tcttcttcgt cgggggcgat
    10801 accacccggt tcaatcagcc gaaaatcacc gtcgcggaaa ttgctcttcg cctgtgggtt
    10861 ggtctcggga atataggcca ggttacggaa cgcgtgtttc accagccggt cttcgagcga
    10921 gtgaaagctg atcaccgcca tacgcccgcc gggtgcgagc tgcctgggga gcatctcgag
    10981 cgctgtgcgc agtacgccca gctcgtcgtt tacggcaatg cgcagcgcct ggaaaacacg
    11041 cgtagcaggg tgccggtctg cgtgcccctt tgccttgtat ttcggtggat aaatcgcctt
    11101 gcagatttct gcgagctgtg tcgtggtcat caccggttta gtacggcggc gcgcgacaat
    11161 ggcacgggca atacggcgcg agagtttctc gtcgccatat tcaaaaaaga tacgcgcgag
    11221 gccctcttca ctttctcgct gcagaagctc ggcggctgaa atgcccgcgc tggtgtccat
    11281 gcgcatgtcg aggggttggt caaaacggaa gctgaagccc cgctcgaaat agtcgaagtg
    11341 tgccgaagaa attcccaggt cgagaaagat aaaacccagc ggcgcttcgt ctgcaaacgg
    11401 gatttcggca aaattggcgt gccggtacac tgccaccctg ttttgcacac gctcgcgtgc
    11461 gagttcaagc atggctgcat cggcgtctga ggcgagcaga cgcgcatcgg gatattgttc
    11521 agaaatagcg agcaggtgcc cgcccagccc ggtagtgcca tcccatacct tgcgctgccc
    11581 gggtggcagc gcagagaggc attctgccgc acggacaacc gcgtcgcgca gaatgctctc
    11641 gtgaaacgga actcgttcct cttctatttt tgggttacct cgggctcagc ctgaggctct
    11701 gcctcgtctt cgccctcttc gctgcccagc agggagtttt cccagatttc gtcgatcgtt
    11761 gcctcgtctt caaagtattc acctttgatt tcaaggaact tctcggcgct caggttcttg
    11821 aaggtaaagt caagtttctt gttgccatct ttttcggtga ggccgatgtt gatttccacc
    11881 ggctcggtga ttttcttttc cagcagcatc atcgcgagtt cgtcttcaat ctcgcgctgt
    11941 accaggcgct tcagcggccg cgcaccatac ttctcgctat aacctttgtc cgcgaggtag
    12001 ctgcgtgcat cgtctttcag cgtgaggaac atgttcttga ccagcgcgtt gtagttgatc
    12061 tcgtcgattg tcagctcgac aatttcctgg atctcttctt tcgtgagcgg gtcgaagatg
    12121 atcacgtcgt cgatacggtt cagaaattca ggcgagaagt gcttgcgcag ctcttcgaga
    12181 acctgctcgc gcttctgctc ctgcttgttt tccgtttcgc cggcgaaccc aagtttgcca
    12241 cccttttgca gaaaacgtgc accgatgttc gacgtcagga taatgattgt ctccttgaag
    12301 tcgacgtggt ggcccgcgtt gtcggttagc tcgccttcat cgagcacctg cagcaggatg
    12361 ttctgtattt cggggtgtgc cttttcgatt tcgtcaaaga gcagcaccga gtgcgggtgg
    12421 cggcgaatgg cttctgtcag ctggccgcca tcttcgtggc cgacataacc cgggggcgaa
    12481 ccgataaact tcgaaaccga atgcatctcc atgtactcgc tcatgtcgaa gcgaatcagg
    12541 gcttcttctt cgccgaacat gaattctgcc agtgccttcg caagctcagt cttacccacg
    12601 ccggttggcc cgagaaagaa gaagctgcca atgggtttcc tgacggattt caggcccata
    12661 cgtgcccggc gtactgcgcg cgatatctgg tcgatagcgg tcttctggcc tacaaccctg
    12721 tcggcaagtt cgtcagccat tttgatcagg cgctcgttct cgctttcatt gagctgttgt
    12781 agcggaatcc ctgtccagtc ggcgatgact tttgaaatat cttctgcggt aatttcgacg
    12841 cgcgcctctt tctggctgcg gcgccacgag ttcatcgcgt cggcgaggcg tgcattggct
    12901 tcgccgatct ggtcacgcag ttttgctgcc ttctcgtatt cctgctggcg caccatttcg
    12961 ttcttgtcac gcgtcagctg ctgtatttct ttttcgatct ctttgacttc ttcagggatc
    13021 actgccgact tgaggcgtgc atgcgaacct gcttcgtcga tgatgtcgat ggccttgtcg
    13081 ggcaagaagc ggtctttgat gtagcggtca gagagtttga ccgccgcaac gattgcttct
    13141 ttgctgaagc tcaccgcgtg gtgtttttcg tacgcatggc gcaggccagc caggatgcgg
    13201 atcgcatctt caaccgaagg ctcttcaacc atgacagact ggaaacgccg ctcgagcgcc
    13261 gaatcacgtt cgatgtagcg tttgtactcg cgcagggttg tcgcgccaat acactggatt
    13321 tcaccgcgcg cgagcgccgg cttcaggata ttcgccgcgt cgaccgctcc ctcggctgca
    13381 ccggcaccga tgatcgtgtg tacctcgtct ataaagagga tcacttcttt attggtacga
    13441 atctctttca tgatcttctt gatgcggtct tcgaactcgc cgcggtactt ggtgccggcg
    13501 atcagcgcgg caacgtcgag tgaatagacc tggcggttga acatcggctc gggtacgcgt
    13561 ttctgcgcaa tgcgttgtgc gaggccctcg gcaattgccg tcttgcccac gccagcctcg
    13621 ccgataagga tcgggttgtt cttcgtcttg cgtgaaagaa tttgtatcac ccgctcgatt
    13681 tcgtggtcac gcccgattac cgggtcagtg agcttttctt ctgccatacg cgacaggttg
    13741 cgcgcaaact cgtcgagcat cggcgtcttc gaagcttctt tctttggctg cgctgcactc
    13801 gattgtgtct tttgtgtcga tgtcgcctgt ggcaggccca gcgtcgcatt caattcattg
    13861 cggataacct ggtagttgac ctggaaggtc tgcagtgaac tggcgacaac accgttggtg
    13921 tcgcgcagca gtgcaagcag cacatgctct gaaccgatat agctgtggcg catcttgcgg
    13981 gcttcgtcgc gcgatagctc gagaatacgc tgtaccttgt ctttgctggt agggtcgatt
    14041 accatcgtcg cgctatcctg ctctgagcgc ttttctacct cttttttcag ctcgttcagg
    14101 tcgatgccta agttcatcag catcttgact gccactgaat cctgcgcctt gagcaggccc
    14161 agtagaatgt gctctggacc taagaactca tggcccaggc gttttgcctc ccgcggagcg
    14221 tattcgttaa taatcttttt aacgcggttg gtgaaatcga acatatggct atattaattt
    14281 cggcagacat gaccgtcaat aaaaagaccc ctaagctctg aattcgccaa attgagtcaa
    14341 ttttgtagcc ataaactgac gaaaatcagt aatctggtca aaaggttcct gtgaaatccc
    14401 tgtttaaacg ccgcactaaa aaacacgatg attcgatagc agggacggag cgcccgtggg
    14461 agtcctgcct cgcggtctgc agcaagtgcg cccgcaaggt aaaggcgatg gatggcgata
    14521 agacaaagct ccgggtggga ctaaaagctc tggtatcgtt gcgcggcctc aaaaagaaac
    14581 tccgccccgt tgacattacc tgcctggacg tttgccccga ggacaagatt gtcgttgccc
    14641 acatgacacc cggtggggtg accctgaaaa tcgttggtcc cgacgccaag cccgaacagg
    14701 tactcagcga gttcggcttc taacaggctc atgaacaagg agccccaagc aggttccgga
    14761 acctgctaaa gatacaaaaa cttccgggcg aatgtggtat gcccggcaaa ggccagctct
    14821 tcgggctcgc gcataacgcg cagatcgaga gcttcttctg atgattcaaa agcaaaatcg
    14881 ctcagccggt agctgctgcg tgtaaccgag aacagtataa tctgtgagtt ctgcgcgaca
    14941 ctgagtgctt cgacgagttc gatgccggcg gggtcaatgc gcaacccggt ctcttcgaaa
    15001 acttcacggc acgaaccctc ttgccaggtt tcgccggttt cgagaaaccc cccgggcaac
    15061 gcccatttgc ctttcgcagg ttcaatgccc cgctttatga tgagaactcc accttcggcg
    15121 cgcaccgcga cgactgcaac cggcagcggg ttcgaccaga cttgtcggcc acatgcgggg
    15181 caggtgcgcg gaaaactctc gacctgcggg tactgtgacc cacagtaatg gcagtgccgg
    15241 tgtgcgttca cgaggtctct tcttacgtgc cgacctgatg gcaaggcgat atacgttttc
    15301 tacgggttgc atgaaattat cgacgtggtt tcctgcttcg acagaagggc agaagatggc
    15361 actgcgtacg tttattatcc tcctgttctt gttgtgtttc gctagctgca aaaagaaggc
    15421 gcctctcgat tattttcctg tcgaagaacg cggaggtaat attgtgatgc gcgagtttgc
    15481 ccgcgattcc gaaaaaagct cccactatgt cttaatgaat ggcgccgagc ggccacattt
    15541 tcatgaccgc cacagtttca tcgtcaggtt agtcagcggc cgcaaccggc tgcacctggt
    15601 ttctgcgacg cacgaacttg cgcccggtga aaccatgcgc attgagaaag ggtcattgca
    15661 ctgggcagag aacgctgggc caggtttttc gattctgcag gtggattttt acccaccatt
    15721 tgacggcaca gaccggcggt ttgcggagta gtgaatggtg gcagtcgggc agtggggtga
    15781 ggggagttct ctcgggagtg cgttcagcag tgcatgggaa gggacgaaga ccctcgccaa
    15841 ccaggcgtgg agtggcgtca ccaacacttt cgagaggaca gtcaacctgg tcgcgggcaa
    15901 tgggtggcat aacaaagccg aatatgaagc cctgaaactt gctcaattga gcggcggttg
    15961 ggaagccagc cgggcattgg tcactggcga tctgtcaagt cctggcctag agggcgattt
    16021 gcgatttaac tttggaatgg gcggtgaagc gcaaatggga caagctctcc ttgcgcagat
    16081 gaggcagaat ggtggtggca ctcaggcaat acttcaaaaa ttcatactaa attcacgcgg
    16141 cgaagcgatt aaggatctcc aagctttggg ggtgaaggtt agttacgaca agaagaccaa
    16201 gcagcttgtg attgatcaaa accagagcga tgatctaaaa aaattggcgg ctgaactcaa
    16261 tttggcgatg aaaacctggg atggtaacgt agcgaatggt atggcaagcg agaacacacg
    16321 gctatataat cagacatgga gtgcaaagtt ttcgcaaatc cacgaaacat catcaggtgc
    16381 gcgtattccg tacggtaacg agttcgttgg cgaccaatac ggttggcgag atttggatag
    16441 cgacccagca accggcaaag atgggaagga agattttcat tacggcactg attttcgcgt
    16501 tggtgcaggc aaatctattc cagctttagc aagcgggagg gtaatcgaac ttgaactcaa
    16561 ccataaaact tatggcaaga caatcctcat tgatcgtggg aacgggctgt attctagaat
    16621 cgcgcacgca tctgaattgg ctgttagaca aaacgattct gtgacaacaa atcaggtcct
    16681 cgggctgaca ggaagcactg gtggcaaatc acttggcccg catcttcacc taatgctcta
    16741 tacgaaggat gagaagaaca atctgaaggc cgtcgatgtc ctcaaagtag attggcgtaa
    16801 gtatgggaga cccagatgag attctggcag actacatgcc ttgtgatatt ggttttcagc
    16861 aattcacagc acagttctga aaccaaagcg aagcatgaag cggtactcga tggtaattgg
    16921 aaaccggtcg gcacaggtta cacggatatg tttttgatga ttcgcagtcg acagaagcaa
    16981 acagaaatgc tggtggtcta cggtggtggg gcaagatatt attctaatgt aaccatagcc
    17041 tgtgaatcaa tgaactgtga cattttcgat ggaggcaaaa agatatgcgc gctgcagatt
    17101 atcacgaatg aaagtcttcg gatggttaaa ccgccagaga tcaaagtcag gcaaaaatat
    17161 ggcagtcaga agattagcac ttttgaattc gtcgaagcta atactgaatt caagagaact
    17221 gagaacacga gctttgactg gccagactaa atttactctg aggttgaatt gaattccctc
    17281 ccgcgcgcca gcacgtttgc cttcggcaaa cccaacgggt cgcccaacat gcttaacacg
    17341 gcgcgaccct tcgactacgc tcagggtacg cttcatggct gcgccacgac gtgttaaaca
    17401 ctgttgggcg ccatgctggt tttttcttag ctgaataatt ttccgacgcg cgtccggggt
    17461 gaagttgtcc gctgcaacgt cgtgttgcag cggtgtcgcc cagcccgtcg ccggcatccg
    17521 tgccggcgcc gacctgggcg acggttggcc tgtcatgaaa caggcaaatt ttaacttttt
    17581 tttcaatttg aattaaaaga taatataacc aactgcgcat ttgcagaaat cggatgcgca
    17641 aggagaaaaa atggcagctt caaaatgtgg tttctgttcg cacacgagat ttgaacttaa
    17701 ggagggcaca cctgtgcatt ctaactataa gatattctat gtgcaatgcg cttcttgcgg
    17761 tgctgttgtc ggtacacaag agtataaaaa tgcaggggcc ttgctggtag agctatcagc
    17821 cgcagttaaa aagattgcgc ggcactttgg cgttcacaca aatctttaag cattgctgcg
    17881 gtttccgatg cagctgtgga aaatctaaga ttttccacag cgttgttgtt gataaagaaa
    17941 atcttccccc cgcgcacggt ggcgggtggg caggcaggct ttgtatat
//
LOCUS       DAOQJF010000015        17802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1497_length_17802_cov_5.356624, whole genome shotgun sequence.
ACCESSION   DAOQJF010000015 DAOQJF010000000
VERSION     DAOQJF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 17802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17802
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1497_length_17802_cov_5.356624"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PLF85_01585"
     CDS             <1..817
                     /locus_tag="PLF85_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNE18375.1"
                     /translation="MALANESSHPLSRAIADFIPRNAESVLSDFIEKPGKGTAARING
                     AKYWLGSQAWLAENGVDVPEVGEAGFSSLVHAAIENHYLGCFLIRNRMRGDLGQTLAV
                     LGAQYRIALLSGDSDRERTRFEQYFSASGELFFSATPARKAEIIAAMRNDGATMMIGD
                     GLNDADALKAADLGVALTENHSNFSPASDAILSAESLYRLPQLIAQAAQARKTAIAAY
                     AVSFGYNIFGIAVAVAGDLTPLFCAILMPISSLSVIGLAFASARLGAAVRRIN"
     gene            817..1017
                     /gene="ccoS"
                     /locus_tag="PLF85_01590"
     CDS             817..1017
                     /gene="ccoS"
                     /locus_tag="PLF85_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNE18376.1"
                     /translation="MEALFVLLIFSLILGVGFLVAFFWAHNSGQFEDSQTPALRMLFD
                     DVDRKRSVHDTYSSQHKKTGGK"
     gene            1021..3156
                     /gene="ccoN"
                     /locus_tag="PLF85_01595"
     CDS             1021..3156
                     /gene="ccoN"
                     /locus_tag="PLF85_01595"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNE18377.1"
                     /translation="MAVETFEYDNRIVNKFALATIVWGVVGMLVGVLVAVLLYIPDYL
                     GHFPILSYGRLRPLHTNAVVFAFAGNAIFMGVYYSLQRLLKARMYSDLLSNINFWGWQ
                     LIIVSAAITLPLGYTSSKEYAELEWPIDIAIALVWVVFGLNMLMTILNRREKHLYVAV
                     WFYIATFVTVAMLHIGNSLAIPVSFMKSYSVYAGVQDALVQWWYGHNAVAFFLTTPFL
                     GIMYYFIPKASNRPVYSYKLSIVHFWALVFIYIWAGPHHLLYTSLPDWAQSLGTVFSV
                     MLIAPSWGGMINGLLTLRGAWDRVRQDPVLKFMVVGVTAYGMSTFEGPMMSLKNVNAL
                     THYTDYIIGHVHLGALAWNGGIAASMLYYIIPRIYNRPLHSLKLANFHFWIGTIGIVF
                     YAIPMYWGGITQSLMWKQFTKEGALLYPNFLDTVTQIIPMYVMRSIGGAMYLTGFIVM
                     AYNMLKTIQAGKLQPGEQASAPSLKDDTSAGHEAPGKFRHRILERKPLVFTGLTLVAV
                     LVGGIIEFVPVAMVRSNIPTIASVKPYTPLELEGRDIYIREGCNTCHSQMIRPFRSET
                     ERYGEYSKAGEFVYDHPFLWGSKRTGPDLHRVGGKYNHAWHFQHMKDPRITSPGSIMP
                     KYPWLFDNALDTSLTTGKIRALSKIGVDYPAHYDKQALTDLNVQAAQIAEVLKKDGMP
                     IEANREIVAVIAYLQRLGTDIKAQKAELK"
     gene            3164..3400
                     /locus_tag="PLF85_01600"
     CDS             3164..3400
                     /locus_tag="PLF85_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18378.1"
                     /translation="MISKTLSDIGNVSIYPVVTLVIFFSIFTLMLIMVLRGNKSQYAK
                     MAALPLEEGQGEAPQRSRQEQASVPAASSIPRVS"
     gene            3411..3986
                     /locus_tag="PLF85_01605"
     CDS             3411..3986
                     /locus_tag="PLF85_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNE18379.1"
                     /translation="MANKNDEKDKLLDHEYDGIRELDNHMPRWWVLGFYFTIVFGLVY
                     MVYYHMAGGPSSDQEYQAEMAASPQKAAAVAVADTGLKPLTDAASLAAGEKLYVAQTC
                     SACHNANLGGLVGPNLTDEFWIHGCDYGTVMNNVKKGFQQKGMMPYGNGKPLQDNELQ
                     QLVSYIFSKRGTNPPGAKAIDPEREKKCAAK"
     gene            4199..>5166
                     /locus_tag="PLF85_01610"
     CDS             4199..>5166
                     /locus_tag="PLF85_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE18380.1"
                     /translation="MYTASDPLQKIEKDTEEYRDHINTIRPDGKRAWVFAKKPKGRFY
                     QWRNLVSYLLLALMFLGPYVRIHGQPLLLVNVLERKFILFGFVFWPQDFYLVVLALLT
                     MILTIVLFTSVFGRIWCGWTCPQTVFMEMVFRKIEYLIEGDFQQQKQLDQSPWNANKV
                     IRKGGKLFVFYAISFAIANTFLAYIIGSEELFKIISEPVTQHVAGFVAINIFTIVFFL
                     VFARFREQVCHFACPYGRFQSALVDNDTISVTYDFKRGEKRGTLGARKKAGFSLVAAA
                     EAGGASQAQLNAGSNPYGDCIDCGSCVVVCPSGIDIRNGIQLECVQ"
     assembly_gap    5167..5266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5267..5665
                     /locus_tag="PLF85_01615"
     CDS             <5267..5665
                     /locus_tag="PLF85_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNE18381.1"
                     /translation="ALVVFMGMTIAFVRVAFSERVDLVSKDYYYRDKEFSGRLEKEKR
                     LLQLGETEISRSADGIVIGLPVYFQGKSIEGKVIFYSPINPAEDFELPLKITGPRANV
                     AARVKTGQKWRVSLDFVSAGQSYFTEKVIW"
     gene            5662..6315
                     /locus_tag="PLF85_01620"
     CDS             5662..6315
                     /locus_tag="PLF85_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE18382.1"
                     /translation="MSFFASALVMGFLSGGHCLGMCGPLVLALPVSEDRVWISVFYRV
                     IYNFGRVFTYTILGGIAGAVAALFSLQVLQARVAQIAGALLILLALLQLLPHIHLGFL
                     SRAHAFIARTIAPLLRNAGRGRFFLLGMLNGILPCGMVAAALLVSIAAETWRSGIAYM
                     AVFGVGTFPLMLLASVFGIYLSGRMRQVVSWAGPLYAIALGILLLLRPALIAPHCAR"
     gene            6380..7456
                     /locus_tag="PLF85_01625"
     CDS             6380..7456
                     /locus_tag="PLF85_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNE18383.1"
                     /translation="MQFHYELISEGDRYYARALRAMRRARHSIQLMVYIWQDDEIGRL
                     FLRAIERKCRQNVRVTVVVDAVGSYELPGNFFRRLQAAGAQVHMHHRFRALSWQWFRF
                     LIRRNHRKIIIVDNELAFTGGFNIMRECSRRYFGERRWLDLAFVSAQTGFVRHLAAQF
                     RDACRRASHEKWARRQLLRSRNRAILLGRRRAVSYSMSRYLKRRLRQARQQIVISVPY
                     FIPYGFYYRILKRKLKRGISVEVLLPESSDVPFVDGVSFYLARKLLKLGAQIYLYHGP
                     GVERRFSHTKFYMVDGFAGTGSANYDYRSMVLNLDTLVFTRAGNPQWRQLSDDMKRFS
                     RLAAEADLRPGLFTRLLLPFRWLL"
     gene            complement(7464..7928)
                     /locus_tag="PLF85_01630"
     CDS             complement(7464..7928)
                     /locus_tag="PLF85_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE18384.1"
                     /translation="MLYSSASKYAVRALAYMTSKADQTVFTVEEIAKGAAVPKPYLSK
                     ILKQLVTGKILKSNKGPGGGYQFARPPGEISLYDIKVTIDGITEFVECALGLDSCNDE
                     APCPAHFIWKDLRAAATKSLQITTLPIANEIMHEKARHPTGQKKTVFKRVKT"
     gene            8066..8980
                     /locus_tag="PLF85_01635"
     CDS             8066..8980
                     /locus_tag="PLF85_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HNE18385.1"
                     /translation="MQIEKAARQLGFARMAALAVTDPAAEYQAFHKDFVARELPQDLY
                     YLRRPERYQIDSLLPGAQTMLLFTYPYRFRHVEEKLRNARYKIARYAWQQDYHDLLKR
                     KLVKLVGEFSLSGRAVTDSAPFAERYWARRAGLGRIGRSGMLIDDQLGSYFLIATLVL
                     TDVLKFSPPPQAAAPASDIESVCGECRLCVEACPTGALRGDSLMDTARCLSFQTIESR
                     ADIGQMPAPIRRHSWIFGCDVCQQVCPWNKSVFAAEEFSAEHPLAGQVASGQLPENRT
                     RLKGSVFHRRGVHKLKQNIAAVERTGFT"
     gene            9098..10393
                     /locus_tag="PLF85_01640"
     CDS             9098..10393
                     /locus_tag="PLF85_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNE18386.1"
                     /translation="MSQINLETLGLHAGQTVDPTTGSRAVPLYQTTSYVFKDTEHAAN
                     LFGLKEFGNIYTRIMNPTTDVFEKRIAAIEGGVGAVATASGQAATTTAILNITRPGDE
                     IVSLDNLYGGTYQLFRVTLPLLGRTVKFAKSGDIAAIKAAITPKTRAVYCETIGNPKL
                     DVPDFAALAQVAHEAGVPLIVDNTVGVGLVRPIEHGADIVVMSATKFIGGHGTSIGGV
                     IVDSGKFAWNNGKYPDYTEPDASYHGLKYWDVFGKFEPFGGVNIAFIIRARVLWLRDL
                     GFSVSPFNAWMFLQGLETLALRVKQHSANALKVAEHLKKHPKVSWVNYPGLTDSPNHA
                     IAKKYLGSNFGALIGFGVKGGLEGGKKFINSLKLLSHLANIGDAKSLVIHPASTTHSQ
                     LTAEEQASTGVSPDYVRLSVGLETVEDIIADLDQALAQS"
     gene            complement(10390..10563)
                     /locus_tag="PLF85_01645"
     CDS             complement(10390..10563)
                     /locus_tag="PLF85_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18387.1"
                     /translation="MGKFIGSGYGFAVKKELPPGGSSPFAVSVHSYSLHGKTARYEAF
                     YSALPRGCLPAAS"
     gene            complement(10566..10913)
                     /locus_tag="PLF85_01650"
     CDS             complement(10566..10913)
                     /locus_tag="PLF85_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE18388.1"
                     /translation="MPRFTRDGQTFNNPVEYALKKIGGKWKMPILWRLREQKKRYGEL
                     KASLDGITHKMLSTQLKELERDGLLTRTVYEVVPPRVEYELTRFGKTSIVIIDSLRQW
                     GLAAMKHDGIEHH"
     gene            11159..11542
                     /locus_tag="PLF85_01655"
     CDS             11159..11542
                     /locus_tag="PLF85_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNE18389.1"
                     /translation="MVLGSPTISKQTGWPIGFWAAERTHPMRVFQEAGYEVEITSTAG
                     GKVEMDGYSNPTDKSGYSAHDVISLGYMQQKSFQAALTNTKKFSEVNPSNYVAIFLVG
                     GQSPMYTFKGNTELQKLFVNFYEAG"
     gene            11678..11857
                     /locus_tag="PLF85_01660"
     CDS             11678..11857
                     /locus_tag="PLF85_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18390.1"
                     /translation="MKIQPYWIEDEARKISGTTFKVKPAFTPYAITDGNLVTGQQQNS
                     GALAAELVLELLDKK"
     gene            11858..12355
                     /locus_tag="PLF85_01665"
     CDS             11858..12355
                     /locus_tag="PLF85_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18391.1"
                     /translation="MRALIALLCFYVLTGWHALYANPIDIYAPESQVKGGASLFYNSG
                     NLERVLTRGEFLASHHNPYWAVATNTSYTWGTFGPFLTEDDWSSRNFAYAWPHARIYP
                     FAMHWLETGLRRKITMRNQLGAGGTVALFSSLLPAENCTAGEPDGRSLVPAVAAYCQR
                     LPLAR"
     gene            12515..13132
                     /locus_tag="PLF85_01670"
     CDS             12515..13132
                     /locus_tag="PLF85_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE18392.1"
                     /translation="MKIAVIGSGNVGGALAQKWAAKGHTVTLGVRDAAKFKGNALATI
                     PGISVASVTDAVAQSEIVLLATPATAAIDVAKSLGDTTGKVIIDAMNVVMGRGPAGFT
                     NTAEAILANTQSREVVKSFNTTGYDNMLNTVYGSEKLDMFMAGDSKDAKARVRQLALD
                     AGFAECYDVGGNDKFALMEQFAFFWINLAMMQGQGRSIGFKLLRR"
     gene            13167..13994
                     /locus_tag="PLF85_01675"
     CDS             13167..13994
                     /locus_tag="PLF85_01675"
                     /inference="COORDINATES: protein motif:HMM:NF039926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNE18393.1"
                     /translation="MDPLKVKESQLLQSERLPAEAVQRFIAAFRSRDDWGLFRINPYR
                     FARQEGIEEDLALDLFIHAARHGLVDFSYDMICPRCGGVAHSHHELDEVEAQEFYCSL
                     CTASSSAVLDDQVEVTFSVNSAVRKLSLNPLQDLETYNRYHFSDNYVVSAQLQALRKK
                     LVRHLLVLRPGQSRKVRLQQGRYSSFQFVSMDTNTSTLVRLDRPDGSFDSAAVLAFGA
                     EGFRHKELLSTKTDCEITVQNETRRPAGFLLTTPDLDAIMEVVRQHPTKIQRFLTAR"
     gene            14123..14260
                     /locus_tag="PLF85_01680"
     CDS             14123..14260
                     /locus_tag="PLF85_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18394.1"
                     /translation="MASAPETRNLMAVERLGSESRHAQLKGVGAETLVYRIYSPQVSA
                     L"
     gene            complement(14277..16430)
                     /locus_tag="PLF85_01685"
     CDS             complement(14277..16430)
                     /locus_tag="PLF85_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE18395.1"
                     /translation="MPLIDVTHGVKLYRDESNSILFGCPPEVIKHLMLKGLGSPQVIV
                     LPDTPYRFDTLQNCTEFPLYYFLFVERNFMQGKKLTIVGAPTHLKANRKLLRLTLLGP
                     TRKEYDALGHNPWFEELWREARAISVKDSSGNELGIDDFVDFVPFENGKAHLRNGTTI
                     THTGRDRFDVAGHSVDISFDEKQTPPYDLRSDFIANPPLHFGVTVLGGASGFNADKPC
                     SGLVLHYNSEHMLIDCVPYLEHALNARGMSTTEVRSIFLTHIHDDHCNIFPLLRLSNK
                     VRLIATKEIFWMAITKLSLQTLMPAKDLAAMFHFVEVKPYERTEFFGLSIEPHYTVHS
                     IPTIGATFRMKDGNTTRSIVFIGDNKGFEDIEKMVASGIVRKEKYDNLRQKYTERHDI
                     LFADGGMGILHGNPRDALGSHSDRIVFMHLEKLPAEFDATFSHAVAGKRYNIIGGTTD
                     SYLIRTMNILNATFRNISPEWGTALMNNFRIVTFNSGDIIFKQNEQSKGIIYIILTGS
                     CSVMYHDGRTLSEKARKEAGDFVGEMAVLDENKVRSASIVAATPVTLCAIDENLFHQF
                     LIAENRVEEMRALWQNRSEIEKFFPFTNFADNVNDRIARSAVRMRVAAGDTLVEQGKN
                     DGEFFIILSGEYSVRHNGVEIKVLRGGDMFGEYGSLAETARNATVTALTDGVVLEIAR
                     GEIRKIIESAPIFQFSMREIMMRRSRELKQFAGAK"
     gene            complement(16487..17536)
                     /locus_tag="PLF85_01690"
     CDS             complement(16487..17536)
                     /locus_tag="PLF85_01690"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNE18396.1"
                     /translation="MTTDDLRIEGLKPLIPPAILIEEIPLPYAGQTFVNTARDHVNRI
                     ITGEDDRLLVVVGPCSIHDTKAALEYAEKLLPLRQQLAGELDIVMRVYFEKPRTRSGW
                     KGLINDPGIDNSFRINDGLRIARKLLVDLTAMGLPLGCEFLDTISPQFIADCVSWGAI
                     GARTTESQVHRELASGLSMAVGFKNGTDGNVKIAIDAIHAAQAPHHFLSITKQGVAAI
                     VHTKGNTVGHLILRGSTQGPNYSSEQVKKVSKELVDAGLRPGLIIDCSHGNSQKDHNR
                     QPVVARDIAAQIAAGERSIVGVMLESHLKAGNQKEAPLEKLEYGKSITDACISFEDTA
                     AVLADLAAAIKARRK"
BASE COUNT         3762 a         4750 c         5012 g         4178 t
ORIGIN      
        1 gatggctctg gcaaatgaat cttcacaccc actcagccga gcgattgccg attttatacc
       61 acgaaacgct gaatctgtcc tcagcgattt tatcgagaaa ccgggcaagg gcacggccgc
      121 gcgaatcaac ggggcaaagt actggctggg ttcgcaggca tggctcgcag aaaatggtgt
      181 ggatgtgccc gaggtgggcg aggcgggttt cagctcgctc gttcatgccg ccattgagaa
      241 ccattacctg ggctgctttc tgatacgcaa ccgcatgcgc ggcgatttgg gccagacgct
      301 cgcagtgctt ggggcgcaat accgtatcgc gctgctctcg ggcgattcag accgtgaacg
      361 tacccggttc gagcagtact tttcagcttc aggggagctg ttctttagtg cgacgccggc
      421 gcgtaaggca gagatcatcg cggcaatgcg caatgacggt gcgacgatga tgatcggtga
      481 cgggcttaac gatgccgatg cgctgaaagc agcagacctc ggggttgcgc tgaccgagaa
      541 ccacagcaat ttctcacccg ccagcgatgc gattctttcg gcagaatcgc tgtaccggct
      601 gccgcagttg attgcgcagg cggcgcaggc gcgcaagact gcaattgcgg cgtacgcggt
      661 atcattcggc tacaacatct tcggcatcgc cgtcgcggtt gcgggtgacc tcaccccgct
      721 cttttgtgcg atactcatgc cgatcagttc gctgagcgtc atagggctgg catttgcttc
      781 ggcgcgcctg ggcgcagccg tgcggaggat taactagtgg aagcactctt cgtcttactg
      841 atattcagcc tgatcctggg cgtcggtttt ctcgtggcct tcttctgggc gcataacagc
      901 ggccagtttg aagattcgca gacccccgcc ttgcgcatgt tgttcgacga tgtcgatcgc
      961 aagcgcagcg ttcacgatac gtattcatct caacataaaa aaactggagg aaaataaaac
     1021 atggcagtcg aaacctttga gtacgacaac cggatagtca acaagtttgc gcttgcgacg
     1081 attgtctggg gcgtcgtcgg catgctggtt ggggtgctgg tagcagtatt gctctacatt
     1141 cccgactatt tggggcactt ccccatactt tcgtacggca gattgcggcc gttacacacc
     1201 aacgcggtgg tcttcgcctt tgcgggcaat gcaatcttca tgggggtgta ctattcgctg
     1261 cagcgtctgc tgaaagcgcg tatgtattca gacctcttgt cgaacatcaa cttctggggt
     1321 tggcagctga ttatcgtatc ggcggcaatc actctgcctc tcgggtatac ctcgagtaaa
     1381 gagtacgcag aacttgagtg gcctatcgac atcgcgattg cgctggtctg ggttgttttc
     1441 ggcctcaaca tgctgatgac gattctcaac cgtcgtgaaa agcacctcta tgttgcggtg
     1501 tggttctata ttgcgacgtt cgtaacggtt gcgatgctcc atatcggtaa ctcgctcgcc
     1561 attccggttt cgttcatgaa aagttattcg gtctatgccg gtgtacaaga tgctctcgtc
     1621 cagtggtggt acggacacaa cgcagtcgcg ttcttcctca cgacgccatt tctcggtatc
     1681 atgtattact tcattccgaa agcctccaat cgcccggtct attcttacaa gctctccatt
     1741 gtccacttct gggctctggt gtttatctac atctgggctg gtcctcacca cctgctctat
     1801 acttcgctgc ctgactgggc gcaatcgctc ggtacagttt tctccgtcat gctgattgct
     1861 ccttcgtggg gcggtatgat caacggtctt ctgaccctgc gcggcgcgtg ggatcgcgtg
     1921 cgccaggatc cggtactcaa gttcatggtg gtcggtgtta ctgcttacgg tatgtcaaca
     1981 ttcgaaggcc cgatgatgtc cctcaagaac gtcaacgcgc tgacccacta tactgactac
     2041 atcattggtc acgtgcacct cggcgcgctg gcgtggaacg gtggtatcgc agcatcgatg
     2101 ctgtactaca ttattccgcg tatctataac cgtccgctgc acagcctgaa gctggcaaac
     2161 ttccatttct ggattggtac catcggtatc gtgttctatg caattcccat gtactggggc
     2221 ggcattacac agagcctgat gtggaaacag ttcactaaag aaggtgcgct gctgtaccca
     2281 aactttctcg atacagtgac gcagattatc ccgatgtacg tgatgcgttc tatcggtggt
     2341 gcaatgtacc tgaccggttt tatcgtcatg gcatacaaca tgctcaagac cattcaggca
     2401 ggcaaactgc agccgggtga gcaggcaagc gctccttcgc tgaaagacga tacgtcagca
     2461 ggccacgaag cgcctggcaa atttcgtcac cgcattctcg agcgcaagcc gctggtcttt
     2521 accggcctga cgcttgtcgc tgttctcgtg ggcggaatca tcgaatttgt ccccgtggca
     2581 atggtgcgtt cgaatattcc aacgatcgca agcgtaaagc cttacacccc gctggagctc
     2641 gaaggccgcg atatctatat ccgcgagggc tgcaatacct gccattcgca gatgattcgt
     2701 ccgttccgtt ctgagacaga gcgttacgga gaatactcga aggccggtga gttcgtctac
     2761 gaccatccgt tcctctgggg ttcaaagcgt acaggtccgg acctgcaccg cgtcggaggc
     2821 aagtataacc atgcgtggca cttccagcac atgaaagatc cgcgcatcac atcgcctggc
     2881 tcgatcatgc caaagtatcc atggctgttt gataacgccc ttgatacaag cctgacaacc
     2941 ggcaaaatcc gcgcgctcag caagatcggc gtcgattatc cggcacacta tgataagcag
     3001 gcgctgactg acctgaatgt ccaggcggcg cagatcgccg aagtgctgaa aaaagacggc
     3061 atgccgattg aagcaaaccg cgagatcgtt gcagtcattg cgtacctgca gcgcctcgga
     3121 accgacatca aagcccaaaa ggctgagctg aagtgaggtt ggtatgatat ccaaaacact
     3181 cagtgatatc ggtaacgtct ctatctaccc ggtagtgact ctggtgatct tcttcagcat
     3241 ctttaccctg atgctgatta tggtgctgcg cggcaataaa tcgcagtacg ccaagatggc
     3301 agctttgccc ctcgaagagg ggcagggcga agcgccacaa aggagtcggc aagaacaggc
     3361 atccgtgcct gccgcctcct cgatacctcg tgtatcatag gagataagtc atggcaaaca
     3421 agaacgacga aaaagataaa ttactcgacc acgagtacga cggcattcgc gaactcgata
     3481 accatatgcc ccgttggtgg gtattgggtt tctacttcac gatcgtcttt gggctggtct
     3541 atatggtcta ctaccatatg gcaggcggac cttcttctga ccaggaatac caggcagaga
     3601 tggctgcttc tccgcaaaag gctgctgctg ttgcagttgc cgatacgggg ctcaagcctt
     3661 tgaccgatgc ggcttcactg gctgcaggtg aaaaactcta tgttgcgcag acctgctctg
     3721 cgtgccacaa tgcgaacctg ggtggtcttg ttgggccgaa cctgacagat gaattctgga
     3781 ttcacggttg cgactacggt acagtgatga acaacgtgaa gaagggcttc cagcaaaaag
     3841 gtatgatgcc gtatggtaac ggcaaaccgc tgcaggataa cgaactgcag cagctggtca
     3901 gctacatttt ctcgaagcgc ggtactaatc cgccgggggc gaaagcgatc gaccctgagc
     3961 gcgaaaagaa gtgcgcggct aagtagcacc aaaccacctt cccggagact tccgggaagg
     4021 tgggctcttg agaccaccgc ggggttgtta gctgaatgca gacaatcccg ccgtatcctc
     4081 aagtgatctg cggaggaagt gacctactca gaggctttat acccatagcg gtatattgct
     4141 cgcgtcacct tcgtgcaaag aaaggcgaca ggatacgtcg aagactatag ggggatacat
     4201 gtatacggct tctgatccac tgcagaaaat tgaaaaggac actgaagagt accgcgacca
     4261 tattaacacg atacggcctg acggtaaacg tgcctgggtt tttgccaaga agccgaaggg
     4321 ccggttctac cagtggcgca acctcgtgag ctacctgttg ctggcgctca tgttcctggg
     4381 accctatgtc cgtattcacg gccagccatt gctgctggtc aatgtgctcg agcgcaaatt
     4441 cattctcttt ggctttgttt tttggccgca ggatttttac ctcgtcgtgc tcgcgctgct
     4501 caccatgatt ctgacaattg tcctgttcac gtcggttttc ggtcgcatct ggtgcgggtg
     4561 gacctgccca cagacggtct tcatggaaat ggtgtttcgc aaaatagaat accttattga
     4621 aggggatttt cagcaacaga aacaactcga tcagtcgccc tggaatgcga acaaggtgat
     4681 ccgcaagggc ggaaagctgt tcgtctttta tgcgatttct tttgccattg cgaatacttt
     4741 cctcgcgtac attattggca gcgaagaact gttcaagatc atttctgaac ccgtcacgca
     4801 gcacgttgct ggatttgttg ccatcaatat ttttacgatt gttttttttc tggtatttgc
     4861 ccggtttcgc gagcaggtct gtcacttcgc ctgcccgtat ggtcgcttcc agtcggcgct
     4921 ggtcgataac gacaccataa gcgtaaccta tgatttcaag cgcggcgaaa aacgcggaac
     4981 cctgggcgca cgcaagaaag cgggattttc gctcgtggcg gccgcagagg ccggtggcgc
     5041 ttcgcaggcg cagctgaacg cgggttcaaa tccatacggc gactgcattg actgtgggtc
     5101 gtgcgtggtc gtttgccctt caggaatcga tatccgtaac ggcatacagc tcgaatgtgt
     5161 gcagtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcac tcgtcgtttt
     5281 catgggcatg accatcgctt ttgtgagggt cgccttttct gaaagggtcg acctggtctc
     5341 caaagactat tactaccgcg acaaagaatt ctctggccgc ctggaaaaag aaaagcgcct
     5401 gctgcaacta ggcgagacgg agatcagccg ttctgctgac ggtatcgtga tcggcttgcc
     5461 tgtctatttc cagggaaaaa gtatcgaagg gaaggtgatt ttctattcgc cgatcaaccc
     5521 ggcagaagat tttgaactcc ccctgaagat aacgggaccg cgtgctaacg tcgcagcccg
     5581 cgtcaaaacc gggcagaagt ggcgcgtctc gctcgatttt gtttctgcgg gtcagtctta
     5641 cttcactgaa aaggttattt ggtgagtttt tttgcctcgg cactggtcat ggggtttctc
     5701 tccggcggcc attgcctggg tatgtgcggc cccttggtcc tggcgctgcc ggtcagcgag
     5761 gatcgagtct ggatttccgt tttttaccgg gtgatctata atttcggacg cgtgtttacc
     5821 tacacaatcc tgggtggtat tgccggcgcc gtggctgcgc tgttcagcct gcaggtactg
     5881 caggcccgcg tggcgcagat tgcaggtgca ctgctcatat tgcttgctct gctgcagttg
     5941 cttccccata tccatcttgg ttttctgtcg cgtgcacatg cgtttatcgc caggactatc
     6001 gcgccgttgc tgcgcaatgc cggccggggt cgctttttcc tgctgggtat gctcaatggc
     6061 attttaccct gcggcatggt tgcggcggct ttactggtca gcatcgccgc ggagacctgg
     6121 cggagcggta ttgcctacat ggcggtcttt ggggtgggca cttttccgct gatgctgctc
     6181 gcctcggttt tcggcatcta cctgagcggc cgtatgcgac aggtcgtttc gtgggcaggg
     6241 ccgctttatg cgatcgcact cggcattctg ctgctgttgc ggcctgcgct gattgcgcct
     6301 cactgcgcgc gttaaccagc actgcattca ccctcaacca actgattgcc gcagtatctt
     6361 cactaccaat agctggattg tgcagtttca ctatgagctg atcagcgagg gtgacaggta
     6421 ctatgcccgc gcgctccgcg cgatgcgtcg cgcacggcat agtattcagc tcatggttta
     6481 tatctggcag gacgacgaaa ttggccgatt gttcctgcgc gcgatcgagc gcaagtgccg
     6541 gcagaatgtc cgtgtaacag tggttgtcga tgcggtcggc agttacgagc ttccgggtaa
     6601 ttttttccgc aggctgcagg ctgccggcgc gcaggtccat atgcaccacc gctttcgtgc
     6661 gctcagctgg cagtggtttc gttttctgat caggcgcaac caccggaaga ttatcatcgt
     6721 cgacaacgag ctggcgttta ccggcggctt caatatcatg cgcgaatgct cgcgccgcta
     6781 ctttggcgag cgccgctggc tcgacctcgc cttcgtgagt gcgcagaccg ggtttgtccg
     6841 gcaccttgcc gcgcagtttc gcgatgcctg ccgccgggca agccacgaga aatgggcgcg
     6901 gcggcagttg ctgcgcagcc gcaaccgcgc gattctgctg ggccgccgcc gcgcggtatc
     6961 ttattcgatg tcgcgctacc tcaagcggcg cttgcggcag gcacgacaac agatcgtgat
     7021 ttcggtgccg tattttatac cgtacgggtt ttattaccgg atactcaaac gtaaactgaa
     7081 gaggggaatc tccgtcgaag ttttgttgcc cgaatcttcg gacgtgccgt ttgtcgatgg
     7141 cgtatcgttc tacctggctc gcaagctgct gaagcttggg gcgcagatct atctctacca
     7201 tgggccgggt gtggagcgcc gcttttcgca cacgaagttc tatatggtgg atggttttgc
     7261 cggtacaggc tcagccaact acgactaccg gagcatggtg ctgaatctcg atacgctggt
     7321 ttttacccgg gctggtaacc cgcaatggcg ccagttaagc gacgacatga aacgctttag
     7381 ccggctggcg gcagaagcag acttgagacc tggcctattc acccggctgt tgctgccatt
     7441 ccgctggtta ctttgaacag cgatcaggtc ttaacgcgct taaaaacggt tttcttctgc
     7501 ccggtcggat gccgggcttt ttcgtgcata atttcgttgg cgatcggaag cgtcgtgatc
     7561 tgcagtgact tggtcgctgc ggcgcgtagg tctttccaga tgaagtgcgc cgggcaggga
     7621 gcttcatcgt tacaagagtc gagacccagt gcgcactcga caaactcggt aatgccatcg
     7681 atcgtgacct taatatcgta aaggctgatt tctccgggtg ggcgcgcgaa ctggtagccg
     7741 ccgccggggc ctttattgga tttcaggatc ttgccggtta ccagctgttt gagtatcttg
     7801 ctcaggtaag gttttggcac ggccgcgccc ttggcgatct cttcgaccgt gaacactgtc
     7861 tggtccgcct tggatgtcat gtaggccagt gccctgaccg cgtatttgct cgcgctactg
     7921 tatagcataa tttcagaata gtgtatctac aattctgttc gttttgtcaa ctcatttgtt
     7981 cgttttcgcc atctggcttt tcagctgacg tgctgagtgc ctgtgacgag aggaaaatat
     8041 ccgagagctc gctgaaaatc accttgtgca gatcgaaaaa gcggcgcgcc agctcggctt
     8101 tgcccgcatg gcagcgcttg ctgtcaccga cccggcggca gagtaccagg catttcacaa
     8161 agattttgtc gctcgcgaac tcccccaaga cctgtactac ctgcgccgcc cggagcgtta
     8221 tcagatcgat agcctgctac ccggtgcgca gacgatgctg ctgtttactt atccctatcg
     8281 cttcaggcac gtcgaggaaa aacttcggaa cgcgaggtac aaaattgccc gctacgcctg
     8341 gcagcaggac taccatgacc tgctgaagcg caaactcgtt aaacttgtgg gggagttttc
     8401 cctgtcgggc agggctgtca ccgattcggc gccttttgcc gagcggtact gggcgcggcg
     8461 cgcaggcctg ggcagaatcg ggcgcagcgg catgctgatc gatgaccagc tgggcagtta
     8521 ttttctgatc gcaacgctgg tgctgaccga cgtgctgaaa ttttcaccac caccacaggc
     8581 ggccgcgcct gcaagcgata tcgaatctgt ctgcggcgaa tgccggctct gcgttgaggc
     8641 gtgtcccact ggtgcactgc gcggcgattc gctgatggat actgcgcgct gtctttcgtt
     8701 ccagacaatt gaaagccggg ctgacatcgg ccaaatgccg gcaccgattc gcaggcacag
     8761 ctggattttc ggttgtgacg tctgccagca ggtttgcccc tggaacaaat cggtgttcgc
     8821 agccgaagaa ttcagcgcag aacatccgct cgccgggcag gtcgccagtg gccaattgcc
     8881 tgagaaccgc acgaggctca aaggatcggt ctttcaccgc cgtggagtgc acaagcttaa
     8941 gcagaatatc gcggcggtag aacggacagg ttttacctga ttgaggtctg cgctgcgggt
     9001 cgcaaaaact tctcgcagga cctctgtttt atatattgtc ctataatcca aaataacgga
     9061 cacgcagtgt ccgctatttt tagcaacagg agcaaaaatg tcacagatca atctagaaac
     9121 tttaggcctg cacgcaggcc agaccgtaga cccaaccacc ggctcgcgcg ccgtgccgct
     9181 ttaccagaca acctcgtatg tctttaaaga caccgaacac gcggcgaacc tctttggtct
     9241 caaagaattc ggcaacatct acacgcgtat catgaacccc actaccgatg tcttcgaaaa
     9301 acgcatcgcg gcaatcgagg gtggtgtagg cgcagtggcg acggcttcgg gccaggccgc
     9361 aacgactacc gccattctga atatcacgcg cccgggcgat gaaatagtct cgctcgacaa
     9421 cctttacggc ggtacttacc agctgtttcg tgtcacgctg ccgctgctcg gtcgcacggt
     9481 gaagtttgcg aagtcgggtg atatcgcggc gatcaaggcg gcgattactc ccaaaacgcg
     9541 cgcggtctac tgcgaaacga ttggaaatcc caaactcgat gtacccgatt ttgccgcgct
     9601 cgcacaggtt gcgcacgaag cgggagttcc cctgatcgtc gacaacacgg tcggtgtggg
     9661 cctcgtacgc ccgatcgaac acggcgcaga catcgtggtg atgtcggcga cgaaatttat
     9721 cggcggccac ggcacttcaa ttggtggcgt aattgtcgat tcaggtaaat tcgcctggaa
     9781 caacggcaag taccccgact acacagagcc agacgcaagc taccacggcc tcaagtactg
     9841 ggacgtgttc ggaaagttcg aaccgttcgg cggagtaaac atcgccttta tcatccgcgc
     9901 acgcgtactc tggctgcgcg acctcggttt cagcgtgagc ccattcaacg cctggatgtt
     9961 tttgcagggg cttgagacgc tggcgctgcg cgtgaagcag cacagcgcca acgcgctgaa
    10021 agtcgcagaa catctcaaga agcacccgaa ggtgtcgtgg gtgaactacc caggtctgac
    10081 tgatagcccc aaccacgcga tcgcgaagaa gtacctcggc agcaacttcg gcgcgctgat
    10141 cgggttcggc gtgaaaggtg gcctcgaagg tggcaagaag ttcatcaact cgctgaagct
    10201 gctttcgcac ctggcgaaca tcggcgatgc gaagtcgctg gtcattcacc cggcatcaac
    10261 cacgcactcg cagctaactg cagaagagca ggcgtcgacc ggtgtctcac ccgactacgt
    10321 gcgcctgtcc gtcggcctcg aaaccgtcga agacattatc gccgacctcg accaggcgct
    10381 ggcgcagagc taagaggctg ctggcaagca gcctcgtggc agcgccgagt agaaggcttc
    10441 gtaccgcgcg gttttgccgt gcagcgaata cgagtgcacc gagacggcaa aaggggagct
    10501 cccacctggt gggagctctt tttttacggc gaagccgtaa ccactgccga tgaattttcc
    10561 cacgctcaat ggtgttcaat gccgtcgtgt ttcatcgcgg ccaggcccca ttgccgcagg
    10621 gagtctatga tcactatgga ggtcttgcca aatcgcgtca gttcgtactc gacacgcggc
    10681 ggcacgactt cgtagacagt acgggtcagc agcccgtcgc gctcgagctc tttcagctgt
    10741 gtggacagca tcttgtgcgt gatgccatcc aggcttgctt tcagttcacc gtagcgcttt
    10801 ttctgttcgc gcaggcgcca gaggattggc attttccatt ttccgccgat tttttttaga
    10861 gcatactcaa cggggttgtt gaacgtttgt ccgtcacggg taaagcgggg catattgcaa
    10921 ggcccgattc tgacctgcac tgccaatcat ataacccggg agcctatact aacttaaatg
    10981 ttagtactaa ctaaaaataa tatatagacg atatacggag ttttgtgcat attggcgtca
    11041 ctatgaaaaa agcacgactc atactactcg caggccttgc cgcactggca gcctgcaaaa
    11101 cgactgaacg acaagctacc agcatcctca tggagtagca cccaagggta agatcctgat
    11161 ggtactggga tcaccgacca tctcaaagca gactggctgg ccgatcggct tctgggcggc
    11221 agaacgcacg cacccgatgc gcgtattcca ggaagccggc tacgaagtcg aaatcaccag
    11281 caccgccggt ggtaaggtcg agatggacgg ctactcgaac cccaccgaca aaagcggtta
    11341 ctcggcgcat gatgtgatct cgctgggtta tatgcagcaa aaatcgtttc aggctgccct
    11401 tacgaacaca aagaaattca gtgaagtcaa tccttcgaat tatgttgcga tatttctggt
    11461 aggcggtcag tcgcctatgt acacattcaa gggaaacaca gagctgcaaa agttgttcgt
    11521 gaacttctac gaagcgggct aacctgcggc agctgtctgc cattcaacga cactgctgct
    11581 cgaagcgaaa aaaagcaacg gcgacctgct ggtcaaaggt aagacctgga cggggtttgc
    11641 caattctgaa gagcagtatg ccgaaaattt cgcgaaagtg aagatacagc catactggat
    11701 cgaagatgaa gcgcgcaaga tcagcgggac cacattcaag gtaaagccgg cgttcacgcc
    11761 gtatgcaatt accgatggta acctcgttac gggacagcag cagaacagcg gggccctcgc
    11821 agccgaactg gtgctggagc tgctcgataa gaagtaaatg cgcgccctaa ttgcgcttct
    11881 ttgcttttat gttctgacgg gttggcatgc actgtacgcc aaccctattg atatttatgc
    11941 tcccgagtcg caggtaaaag gcggagcttc actgttctat aactcgggca acctcgagcg
    12001 ggttttgacg cgcggtgaat ttctcgcttc gcaccacaat ccttattggg cagttgccac
    12061 gaacaccagt tatacctggg gaacctttgg accttttctg actgaagacg actggtccag
    12121 tcgcaatttc gcctatgcct ggccgcatgc gcgcatttac cctttcgcga tgcactggct
    12181 cgagactggg ctgcgccgca aaattactat gcgcaaccag ctgggggcgg ggggaaccgt
    12241 cgcattgttc tcttctttac taccggcaga aaattgtacc gctggtgagc ctgacggccg
    12301 aagtctggta ccagcagtcg ctgcgtactg ccaacgatta ccgctggcac gttgagtcgg
    12361 gtttcgagtt cagactcgca aaagccattg cgctgaagac tatggcgata tggcattttg
    12421 aatcggccgt cccactcggt gtaaaaagat acgatgtgct gtggaccgtg ggagttacgg
    12481 ccgaatggtc atcactgatg gaggaaaata aattatgaaa atagcagtca ttggtagtgg
    12541 taatgtcggc ggtgcgctcg cgcagaagtg ggcggccaaa gggcatacgg taacgctggg
    12601 cgtccgggac gcggcgaaat tcaagggcaa tgcgctcgcg acgatacccg gaatttcggt
    12661 tgcctcggtt accgatgccg tcgcgcagag cgaaattgtc ttgctggcaa cgcccgctac
    12721 agccgcgatc gatgtggcaa agtcactcgg tgatactacg ggaaaggtaa tcatcgacgc
    12781 catgaatgtc gttatgggca gggggcccgc cgggttcact aatacggccg aagctattct
    12841 ggcgaatacg cagagccgcg aagtggtcaa gagcttcaat acgaccgggt atgacaacat
    12901 gctgaataca gtctatggct cagaaaaact cgacatgttc atggcaggtg acagtaaaga
    12961 tgccaaggcg cgcgtccggc agctggcgct cgatgccggc tttgccgagt gttacgacgt
    13021 cggcggtaac gacaagtttg cgctgatgga gcagtttgcc tttttctgga tcaaccttgc
    13081 gatgatgcag gggcagggac gcagtattgg atttaaattg ttgcggcgct gatgtgctct
    13141 gttcataagc cggaataaca aagacgatgg atccgctgaa agtcaaagaa tcacagctgt
    13201 tgcagagcga acgcctgccc gcagaggccg tgcagcgctt tatcgctgca ttccgctcgc
    13261 gtgacgactg ggggctgttt cgtatcaatc cttaccgctt cgcgcggcag gaaggaatcg
    13321 aagaagatct ggcgctcgat ctttttatac acgctgcacg gcacgggctg gtagatttca
    13381 gctatgacat gatatgcccg cgctgtggcg gcgttgcgca ctcgcaccat gaactcgatg
    13441 aagtggaagc gcaggagttc tactgttcgt tgtgtacagc atcgagttct gccgttcttg
    13501 atgaccaggt agaagtgact ttttcagtga attctgctgt caggaaactt tcactcaatc
    13561 cgctacaaga ccttgaaact tacaatcgct accatttttc cgataactat gtggtctcgg
    13621 cgcagctgca ggcgctgcgc aagaaactgg tgcggcacct gttggtgctg cgacccgggc
    13681 aaagccgcaa agtccggttg caacaggggc gttacagtag ttttcagttc gtctcgatgg
    13741 acaccaatac ctcgacgctc gtgcgcctgg atcggcctga tggcagcttt gacagtgcgg
    13801 cagtgctggc ttttggcgcc gagggattca ggcacaaaga actgctgtca accaaaacgg
    13861 actgcgagat caccgtgcag aatgagacgc gcaggcctgc aggctttctg ctgacgacac
    13921 ccgacctcga cgcgatcatg gaagttgtca ggcagcaccc gacgaagatc caacggtttt
    13981 tgactgcaag gtagggctac atgaggggcc cgcgctggcg gtactgcgag acgaacggct
    14041 cgactatttc ggacagacag tgaatatcgc cgcgagggta cagggactcg cctccgcgtg
    14101 tgaaatctgg cttaccgagc cagtggcttc agctccggaa accaggaatc tcatggcagt
    14161 agagcgcctg ggtagcgagt cacggcatgc tcaactaaaa ggcgtcggcg ccgaaactct
    14221 ggtctaccgc atctattcgc cgcaggtgtc cgcgctctag cccagtccgc tggtggctac
    14281 ttcgcccccg cgaactgctt cagttcgcgc gagcggcgca tcatgatctc gcgcatgctg
    14341 aattgaaaaa taggcgccga ttcaataatc ttgcgaattt caccgcgcgc gatctcgagc
    14401 actacaccgt cggtgagcgc agtgaccgtt gcattgcgcg cagtctctgc gagcgagccg
    14461 tactcgccga acatgtcacc gccacgcagc accttgatct cgacgccgtt atggcgcacg
    14521 ctgtattcgc cgctgaggat gataaaaaat tcgccgtcgt tcttgccctg ttcgaccagc
    14581 gtgtccccgg cggcaacgcg catacggact gcgctgcgcg caatacgatc attgacgttg
    14641 tcggcgaagt tggtgaaggg gaagaacttc tcaatttctg accggttctg ccagagagcc
    14701 cgcatctctt caacgcggtt ttcagcgatc agaaactggt gaaacagatt ttcatcgatc
    14761 gcgcaaagtg ttaccggggt tgctgccacg atcgaagcgg agcgcacctt gttctcgtcg
    14821 agtacagcca tttcaccgac aaagtcgcca gcttctttgc gggctttctc gctgagggtg
    14881 cggccatcgt ggtacatgac gctgcagctg ccggttagta tgatgtagat aatacccttc
    14941 gattgttcgt tctgcttgaa gataatatcg ccgctgttga aagtgacgat acgaaagttg
    15001 ttcatcaggg cagtgcccca ttcgggcgag atgttacgga atgtagcgtt gagtatgttc
    15061 attgtacgga tcaggtacga gtccgtggtg ccgccgatga tgttgtagcg tttgccggcg
    15121 acagcatgcg aaaatgtcgc gtcaaattca gccggcaatt tttccaggtg catgaagaca
    15181 atgcggtctg aatgcgagcc cagggcgtcg cgcgggttgc cgtgcagaat gcccatgcca
    15241 ccgtcggcga agagtatgtc gtgccgctcg gtatatttct gccgcaggtt gtcgtatttt
    15301 tctttgcgca caataccgga tgcgaccatc ttctcgatat cttcaaaccc cttgttgtcg
    15361 ccgatgaaga cgatactgcg cgtcgtgttg ccgtctttca tccggaaggt tgccccgata
    15421 gtgggtatcg agtgcaccgt gtagtgtggt tcaatcgaaa gcccgaaaaa ctcggttctt
    15481 tcgtatggct ttacctcgac gaagtggaac atggctgcca ggtctttggc cggcattagc
    15541 gtctgcagcg acagcttggt aatggccatc cagaaaattt ctttggtcgc gataagtctt
    15601 accttgttcg acagacgcag caggggaaag atattgcagt ggtcatcatg gatgtgcgtg
    15661 agaaatattg agcgcacctc tgttgtcgac atgccgcgcg cattcaaggc atgctccagg
    15721 tagggcacgc agtcgatcag catgtgctct gaattgtagt ggagcaccag ccccgaacac
    15781 ggcttatccg cattgaagcc cgatgctccg cccaggaccg ttacgccaaa gtgtagcggg
    15841 ggattcgcga tgaaatcact tcgcaggtcg tagggtggag tctgcttttc gtcgaagctg
    15901 atgtcaacag aatggcctgc tacatcaaaa cgatcgcgac cggtgtgtgt gatggtggtg
    15961 ccgttcctaa ggtgcgcttt accattttca aacggaacaa aatccacgaa atcgtcaata
    16021 ccgagttcat tgccggagct gtcctttact gaaatggcac gcgcttcgcg ccagagttct
    16081 tcaaaccagg gattatgccc cagcgcatca tattctttgc gggtcgggcc cagcagggtg
    16141 agccgcagca gtttgcgatt cgctttgagg tgagtgggag ctcccactat tgtcagcttc
    16201 ttgccctgca tgaagttgcg ctcgacaaaa agaaaatagt aaagaggaaa ctcggtgcag
    16261 ttctgcagcg tgtcaaaacg gtagggagtg tcaggcagga cgatcacctg cgggctgccg
    16321 agccctttca gcatcaggtg cttgatgact tcggggggac agccgaacag gatactattg
    16381 ctctcgtcgc gatagagttt caccccatgc gtgacgtcga taagtggcat ggggttaagt
    16441 gagaatcagc aacccgctcg caagcgaatt tgccggcgag tggctattac tttctgcgtg
    16501 ctttgatcgc cgcagcgaga tcagccagta ccgctgccgt gtcttcgaaa gagatacatg
    16561 cgtctgtgat cgacttgccg tattcaagtt tttcaagcgg agcttctttc tggttgcctg
    16621 ctttgaggtg gctttcgagc atcacgccca cgattgagcg ctcacccgcg gcgatctgcg
    16681 ccgcgatatc gcgtgcaaca acgggctgcc ggttgtggtc tttctgtgaa ttgccatggc
    16741 tacagtcgat gataagcccg gggcgcaagc ctgcatccac cagctctttt gacactttct
    16801 ttacctgctc gctgctgtag ttggggccct gtgtcgaacc gcgtagaatt aaatgcccga
    16861 ctgtattgcc ctttgtatgg acgatggcag cgaccccctg tttggtgatc gagagaaaat
    16921 ggtggggggc ctgcgctgcg tgtatcgcgt cgatagcgat cttgacattg ccgtctgtgc
    16981 cattcttgaa gcccacggcc atcgacaggc ctgaagcgag ttcgcggtgt acctggcttt
    17041 cggttgtgcg cgccccgatg gcgccccagc tgacgcaatc agcgatgaat tgggggctga
    17101 tggtatcgag aaattcgcag cccagcggca ggcccatcgc ggtcaggtca acgagcagct
    17161 tacgtgctat gcgcaggccg tcattgatgc gaaatgagtt atcgataccc gggtcgttga
    17221 tgagtccctt ccagcccgag cgcgtgcgcg gcttttcgaa atatacccgc atgacaatgt
    17281 cgagctcgcc cgcgagttgt tgccgtaacg gcagcaactt ctcggcatat tcaagcgcag
    17341 ctttggtatc gtggatagag cagggaccta caaccacaag caggcggtcg tcttcaccgg
    17401 tgataatgcg gttcacgtgg tcgcgcgctg tgttgacgaa ggtttgtccc gcgtagggca
    17461 gcgggatctc ttcaatgagg attgcaggcg gaataagcgg cttaaggcct tctatgcgca
    17521 ggtcgtctgt ggtcattttc tagtctcgga agaattatct ttattaatgg tatctttttc
    17581 gttttttctc agggcagaca gaaaaaaagt cgatctgcgg taccggaaaa aaattgcctg
    17641 aacatcgccg gtggattgtc tggagagacc atgtccgggc gatgtatctt cgagggggcc
    17701 ggcttttttc tcagggccct actggctatt accctggcgc tctacctggc acccaccgtg
    17761 tacgcgcact atgggctggc tggcatgctg ccgggtgacg cc
//
LOCUS       DAOQJF010000016        17429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1543_length_17429_cov_5.276102, whole genome shotgun sequence.
ACCESSION   DAOQJF010000016 DAOQJF010000000
VERSION     DAOQJF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 17429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17429
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1543_length_17429_cov_5.276102"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..605)
                     /locus_tag="PLF85_01695"
     CDS             complement(174..605)
                     /locus_tag="PLF85_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNE18397.1"
                     /translation="MKKILLGMIAFGLLSTGLMAEDISGTWMTIDDETGQARSHVNIW
                     VHNGVAYGKVTKLLNRKPNEDPDPVCTECKGDNNGKKVVGMTILWNLKEDGDEWKGGT
                     ILDPKNGKTYKCKIKREGNSLKVRGFIGFSLLGRTQVWKKL"
     gene            complement(675..1211)
                     /gene="pth"
                     /locus_tag="PLF85_01700"
     CDS             complement(675..1211)
                     /gene="pth"
                     /locus_tag="PLF85_01700"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNE18398.1"
                     /translation="MPKKVLLAGLMNPGDKYRFTRHNAGAMALDEVFPGVTYSPNKAL
                     EAHTGQSEEGDMLFFFLKPQTFMNLSGQSVVKAMKKHNVEASHLIVLHDEVELPVAEV
                     RYKFGGGHKGHNGLRSIMAVIGTGDFHRIRIGVGRPADDSHGIADYLLAFQPESQRAK
                     KAQLLPALEMALKAINNA"
     gene            1656..2258
                     /locus_tag="PLF85_01705"
     CDS             1656..2258
                     /locus_tag="PLF85_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE18399.1"
                     /translation="MLVALTMLDDLKDEELIRELNRAELAEHLTGDDAGAERIFTILV
                     GRHNRAFARLVTLRYGVDSASADEIAQDFWLECYSALPRYNPERPFLAWATTILFRTA
                     EKFLRKRSREIQLSPQSDFFDMQASNETPESEAMTREQMAAMLSALRQLPPELALLIQ
                     LRFFENKKIEDITAATGLGRSTIFEKLNLAYRKMRKLMKE"
     gene            complement(2304..3440)
                     /locus_tag="PLF85_01710"
     CDS             complement(2304..3440)
                     /locus_tag="PLF85_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNE18400.1"
                     /translation="MATKTKTKQAEKSAEPKGNAKPAKPVKATKEFAQNGKPFVMIMA
                     GGSGTRLWPMSRVNRPKQLINIVGKKTLIEEAVERARLLTTPDRIYIGTNKDLAAKIQ
                     KIVKLDKKQYLLEPEARNTAPIIAYFTSFLKQHGADDASGVVILAADHHIEPAEKWAE
                     TVKLALTRAGERIWCMGIKPTRAETGYGYIEAGIEVAPRMSAISSFREKPDYQTASHY
                     LTTERHYWNSGMFIFSLGRMREDLRIYQAEMLKLADACAQTPANLAKSFAKMQNISID
                     YAIMEKTKKVGLIQADFLWDDVGSFEALGRIYPVNESGNHVISGKVISHRAKGNIVKT
                     GLTIALLGLDNCVLVEDNGVLLIAKRDALPEIKELREKAPKELL"
     gene            complement(3379..4359)
                     /locus_tag="PLF85_01715"
     CDS             complement(3379..4359)
                     /locus_tag="PLF85_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18401.1"
                     /translation="MLGDFFRRALQILAIASVGSVIYLDVLSVPVGHLVFLQRATGSS
                     STATELQAFSSGYTFVPTLFVPGRWRRYQLETAPRLQEVRVRLPLRYSAYLRLNDLFY
                     VHMRLRIEGEIDEAQATAALAALQMRPTERDRFVEESLQFLAAEYFINLNTDERQLER
                     LKIQLTSFFNGANLPELQKRLDAQLRTPWYRLKKVELREIHVPDSEIYAAQTRNLEQV
                     AAADRRALLAQIEKEAELSVERKRNMEDLAKAEKMSALMAENPTLLEYYKIEKIATRA
                     GQVILDASAHGTEKRISIIPPNEKESARGRNGNEDKNEAGGEIGGAQRQR"
     gene            complement(4386..4628)
                     /gene="hfq"
                     /locus_tag="PLF85_01720"
     CDS             complement(4386..4628)
                     /gene="hfq"
                     /locus_tag="PLF85_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNE18402.1"
                     /translation="MTKNNLQDFILNQIRKEKMGITIYLSNGVPIKGRVVSFDNFTIV
                     LEHDHKQTLVYKHAVTTISPERPIRLHNPETGAEEE"
     gene            complement(4625..5599)
                     /gene="miaA"
                     /locus_tag="PLF85_01725"
     CDS             complement(4625..5599)
                     /gene="miaA"
                     /locus_tag="PLF85_01725"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNE18403.1"
                     /translation="MAQKPRLIILTGTTASGKSAFLYQSLHDLPLAIINADSRQVYRD
                     LRISSASPGSAELGLFYHELYNFLPLDAVYSAGTFVADARQAIARSLAQGRIPVVCGG
                     TYFYIQSLLDGLLPAIPVPETIRAQVEEMSEAEAWQKFNAIDPVAAARVHQHNRVRLN
                     RQLMLCLAHGGPISSIARAGGIADEFEILMLVFVPQREVLRERVSFRLKQMFAAGMVG
                     EADAVVRSALAAGLDWHTLPALTGIGTSEFFDAYASTGLLPAELNTEQLASVEQSIIT
                     NTMQLVKRQMTWFRNSSAKQPFTKTVDPSYEHELIAALARDFMQVRVQ"
     gene            complement(5609..6169)
                     /gene="gmk"
                     /locus_tag="PLF85_01730"
     CDS             complement(5609..6169)
                     /gene="gmk"
                     /locus_tag="PLF85_01730"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNE18404.1"
                     /translation="MDSRPIFVISAPSGAGKNSLINVLLSKEPRLMHSISSTTRARRA
                     NEADGINYHFLSREEFEKRVEGGEFLEYARVLENYYGTEIREIRRIFAQDKYPILDID
                     VQGAQTLRGKPVRMVSIFIIPPSMTELERRLRARASETEEQIRSRLELARLEVMEQNN
                     FDYVVVNDDLMKAAEELATIVRKHMV"
     gene            complement(6243..7172)
                     /locus_tag="PLF85_01735"
     CDS             complement(6243..7172)
                     /locus_tag="PLF85_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE18405.1"
                     /translation="MRLPGLFAVFAALFIAGTLDARKRYDERGNPLAESTTEPQAGSV
                     QAVPVEGKTPDAAAAPPEKSRADAASATAEPANAAEEETAQQQVRNPLTKVYLENAQL
                     YLRSDRTDKALEFLRRSQEAGEDNYSREARLQSYWLRAKRGDTGLAAEAENLDEALRL
                     AASLRVADGYYTCSRELRQKPECLAEAERVYANLAELAPSSHEGRLAAFRLGQLLLDQ
                     GRFEAALPHLTRLLASEPKTPSRKNEVPFDRAWYTLGSLYERPWYHRDTHKARLAYQQ
                     VLRYPGSPYHSQAKERIRWLERFGTGYTRTGSP"
     gene            complement(7172..7921)
                     /locus_tag="PLF85_01740"
     CDS             complement(7172..7921)
                     /locus_tag="PLF85_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18406.1"
                     /translation="MLRRILGNIIPTLDKLIERIPPHIRDIIQKASIAFAALIALLVI
                     ISGINKGLRDAKPAGYKLVERNRDLFYLQEMREEYAKKRQLVEDVEVDPLQFPSRQQA
                     ADDTRFVTMGRETMGHLMGEKEEMLKSEDALRPKEKSPGYLGDSYTVPRLTPDKKTIE
                     DDEAPLTKEKIGSRPPERVVQGEAPAGAAVNPAPRTATQGAGRDIFDGPQGNNRPATT
                     DAARRPAEMPRTTERTAGPTGDARKKLEFMD"
     gene            8054..8569
                     /locus_tag="PLF85_01745"
     CDS             8054..8569
                     /locus_tag="PLF85_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNE18407.1"
                     /translation="MQTLKKKLRRPKYRFWAAFSTAALLLLLEVIPKAAAYARVSPSA
                     PVLPQEDLIRDEVVRQAQSFLNLPYKFGGASPNGFDCGGLVLYLYGRHGFTMPHGVIY
                     MRPTLRLTRTPKKGDVIFFLNDNNIVGHVGMYIDEERFIHAPKEGEVIRYEKLKHPYW
                     KKRTVEIRSVF"
     gene            8808..9848
                     /locus_tag="PLF85_01750"
     CDS             8808..9848
                     /locus_tag="PLF85_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18408.1"
                     /translation="MKKLISIAMVLLTASSAFAWKGQTHNRISQDAIDLLNSMTTNPQ
                     AQAAVQKLVSRYGSLTAAKSAFGNQAKDEDIPQPNFPLGNDMWFDAWWSYSRNGSPSL
                     FGLVDVAYYVALSHFVNAFNASPSADSHNQKPMGYNYYRSRALYNLVGDNFTGDYDWV
                     AEVYNYNLNPTSDTISRMRNLCGGSCRFSTGAFDKDYQDVNHVSGASACAALIDGPSG
                     YVASGNLTLIARCYHYIEDASQPHHAGAYYGKDHTDFEGFVEDNYYNPSYQISQTAAK
                     LAEAQALYAQFANMSAENVVKTMAQWSTTNCYAVYTNDYTSNEIACAEKLLPRIRAAV
                     AAVTAQTIARRP"
     gene            9852..11153
                     /locus_tag="PLF85_01755"
     CDS             9852..11153
                     /locus_tag="PLF85_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18409.1"
                     /translation="MKANFLAIAALCAAVAVSCKGKVTYDLEPPGILLQSGSLSLPTK
                     VFQSMFDVMRKSRIKEGQDPRSAIFMVVADHYLAAEYLKRHSGKPDALIGAEASKVYY
                     ETLAYYLSERNLKQTFMKWVKNTTIPQVPELRALFDDKAEAAYTNIPLRRAQADTLVL
                     ARYGEAGDKTLTYSRLFDSLPHAGQLHLFTSPDRRSLEELVAAQLRREFFDEYVAKAG
                     EGDKSEYLALRQLVENSIYSRALRYEMGMENANPHAENSAVKERAKSVSFSKVKEYYA
                     ANRDKYKEVQTVDCRHIQLKDYDLAINLRDKIDQGTDMVALVKKHSLAADKNSPEPGL
                     IKGIKNDTELHKRPRLETLCMMPKQGESEVVRDGDFFHVVKAERRTDGYPPLDETTHL
                     KDDIAREIAAQELKAEFDAHKKKVLGRVDIRINKTELEKLR"
     gene            11150..11743
                     /locus_tag="PLF85_01760"
     CDS             11150..11743
                     /locus_tag="PLF85_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18410.1"
                     /translation="MKRYHWIALLLLGAIAVYWIARSTGDKKQTRTSRDDVTVVSASG
                     SAHDLQPDMENYRFEREQLLAMPDIGQARVKPEMKNAPEYKASPLRGRVAAGPVDDFG
                     APLFIPSETAMTDPKLFEQELAKHHERADSYAAARMDELRINTEVEKTAMKSNIDQAK
                     AQGSRSPEEIKRAEEALARMEQLQKVLNGEKIENRLE"
     gene            11838..13013
                     /locus_tag="PLF85_01765"
     CDS             11838..13013
                     /locus_tag="PLF85_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNE18411.1"
                     /translation="MIQKLEISQQLRDALQAETRSAQHATREIRIRSTWPGIQLYYPP
                     IKYLPRDNRYETESEARERLLKYARQSEAHTLIFDLEDGCRQKSLSRQFLAMSLPDVR
                     AAIPGRVAVRINPVTSPEHSLDLSLLKEIAPYVDDVMLAKAGEQHGIAELDKLGAFLD
                     SLSPAIRIQPIIEHPRSLKIAGEIFGHQKVEHVVFGIHDFSRALQLMITAENWQDELY
                     PFMCNLMVEARLAGKGIIGGVETLLLSADPPDVALIPEWLNRHAEANATIICNHARRE
                     ASLGFTGKQVIHPNHIRLLRYGFSPGNEDLHRSLRILEEAIKANAFLGGAIRFEDEMM
                     DPPMFAKALQLLMRSVMLGNQDTGVMEVVAGLIASLPTEKFPEIWPYPGWPPLAIQE"
     gene            13040..14605
                     /locus_tag="PLF85_01770"
     CDS             13040..14605
                     /locus_tag="PLF85_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNE18412.1"
                     /translation="MRNQVPEFLNIAEECLAKRAAGNDKNKVAMIFANDKDEAREYTY
                     AELDDLSARFAQSLTERGIAPGARVLLRLPNTVAFPVAFFGSMRAGAIPVPTSPMLTA
                     TEVRYLITNSGADAIVTEEKLWSAVADEIPAREVKALYVSGLSSTTGAINFEDELAAK
                     KPAANIHRARSADAAYLVYTSGTTGYPKGVLHAHRALIGRLPAAENWFHFAGDDRILH
                     SGKFNWTYVLGTAMMDPLYQGKTVIVYEGENSADRWISLIKKFGCNIFIGVPTIFRQI
                     LQKTTAKAEDVPTLRHCMCAGEHLTDEVLDGWISRFGFPIYEGLGMSECSYYISQKKG
                     DEIVKNSAGKIQPGHLVQLLDEDLKPVAAGEEGMICISRNDPGLFVEYWRAAEQTAAQ
                     FKGDWFLTGDYAIQDKNGYIFFLGRRDEIIKSFGYRVSPFEIERVYRDHPALDDIVAF
                     AEHLGPEKTLISMCVQVKGGTTFDEQELLTWGKERLASYKLPKKVYRLDKFPRSANGK
                     VLRSKIPKELGLV"
     gene            14654..15118
                     /locus_tag="PLF85_01775"
     CDS             14654..15118
                     /locus_tag="PLF85_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE18413.1"
                     /translation="MAKRYSGSPEEILQVDAYVKLMRCADTVQGILARNAEANGLTLS
                     QLGVLEILLHLGPQNQKDLAGKILRSGANMTTIIDNLEKREIVRRTRDEADRRTIRVE
                     LTETGEDLISRIFPLHMNNIYRMMGALAPDELHALSQLTRKLGIAARGRMTV"
     gene            complement(15123..15665)
                     /locus_tag="PLF85_01780"
     CDS             complement(15123..15665)
                     /locus_tag="PLF85_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18414.1"
                     /translation="MKNKKFWLVIAVFTLTVPGFILFSYWYLGIFSAVRISPASALDL
                     TVIGGQVTGDYNKTGAEVVKTRQLLEANGFTCDPVLVYYENAITTGKPYLRSAGGCIL
                     AKELPENLLKDLLRQGRQRHRVEFARSVRIETYAQTAVGLRKVWTEIARLSEQGRQLQ
                     FPLVQRIREDGTNEFLIGEK"
     gene            complement(15662..16135)
                     /locus_tag="PLF85_01785"
     CDS             complement(15662..16135)
                     /locus_tag="PLF85_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNE18415.1"
                     /translation="MKPAGWLLKSEPETFSIDNLQASPQKTTLWDGVRNYMARNNLVA
                     MQKGDRAFFYHSSCPVPGIVGEMVVASAAMPDPAQFDAASPYFDARSTPEKPRWFAPK
                     MKFVKKYPATFTREQMVHTSLGSSQLFTHTRLSVIALSAAEIKAIDQWLGALARK"
     gene            complement(16132..16365)
                     /locus_tag="PLF85_01790"
     CDS             complement(16132..16365)
                     /locus_tag="PLF85_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18416.1"
                     /translation="MRYLKSAMPVPGKGTAFMLYEIEGENTIVRMLTHIPETNEIKLY
                     PSPKMKTLFQPERLDEAAKEEFEYIWDMGSEQA"
     gene            complement(16408..>17429)
                     /locus_tag="PLF85_01795"
     CDS             complement(16408..>17429)
                     /locus_tag="PLF85_01795"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNE18417.1"
                     /translation="LVRNPCFGFSAPWSPREAVQVFDHSVSDATLDELVSFGTDIQEF
                     HSKELKKFPLNSLELFLEDFAYTIENHHGLSVRADSTRVVCDYVLTSPDNRHEIMGIK
                     RRRFLKDLALKEQLAEDAAVLADLQVAQLPPTGELPVLLAGDALDSLFDFFVAQLDGH
                     ALFNRYSVFSAGDAVLQNEREPLNIASTPELAGGMHSYSFDELGFPTSRVQLIENSRV
                     KNFLIDGRYADLLRLPRTSALMNIEVDCGTTPYADFLQNGVLELTKFSTFQPNTISGA
                     FSGEIRLGYLHKDGKKIPIKGGSVSGSTQTAFARALKSAERVQRGAYSGPRGVFFESL
                     TVAGS"
BASE COUNT         3909 a         4937 c         4708 g         3875 t
ORIGIN      
        1 accgggcatt tgtggcgctg gcatttgtgt tggctccctc gggggttatg tcccctatcc
       61 gcttttcggc ggatttcgcc aatcccacaa gcgtttttcg taaaaaaggg gtggatatcc
      121 acccctttac ggctctttct gccagggctg ccggtagcag cccggttaag agtttagagt
      181 tttttccaga cctgcgtgcg gccgagaagc gagaaaccga taaagccgcg cactttgagg
      241 ctgttgccct cgcgcttgat tttgcattta tatgttttgc cgtttttcgg atcgagaatt
      301 gtgccgccct tccattcgtc gccgtcttct ttgaggttcc acagaatggt cataccgacg
      361 acttttttgc cgttattgtc gcccttgcat tctgtgcata cgggatccgg atcttcgttt
      421 ggtttacggt tgagcagttt tgtgacttta ccgtaagcaa cgccattgtg aacccagatg
      481 ttcacatgcg aacgcgcctg gcctgtttcg tcgtcgatgg tcatccatgt gcccgaaatg
      541 tcttctgcca tgagccctgt tgacaatagg ccgaatgcta tcatacccag taagattttt
      601 ttcattgaat ttcctccgct tagtggctta acgccaaaag gttatcggct caggtcacgc
      661 gccatgaacc ggggtcaagc attattgatc gctttcaggg ccatttcgag cgccggtaac
      721 agctgcgctt ttttggcacg ctggctttcg ggctgaaatg cgagcagata atcggcgata
      781 ccatgcgagt cgtccgccgg cctgccgacg ccgatgcgta tacggtgaaa atcccccgtg
      841 ccgatgaccg ccatgatact tcgtaaacca ttgtgccctt tatggccgcc gccgaattta
      901 taacgaacct cggctaccgg taattctacc tcgtcgtgta aaacaatcag gtgggatgcc
      961 tccacgttat gttttttcat cgccttcacc acgctctgcc ccgagagatt cataaaagtc
     1021 tgtggcttta aaaagaagaa gagcatatcg ccctcttcgc tctgccccgt atgcgcctcg
     1081 agcgccttgt tcgggctgta ggtgacaccg ggaaaaacct cgtccaaagc catggctccg
     1141 gcgttatgcc gcgtgaaccg gtatttatcc cccggattca tcaggcccgc gagcagcact
     1201 tttttcggca cggtgggtta gtctgaattc gcccttatgc cgccatttct tttttggttg
     1261 caaaagccac tgcgcatcgt ataacggtga gccggtttct gggacgtacg ggctttcttt
     1321 ttctagattt gaaccaatgt aacaaaaccg gaagccaggg aagcgcggcc aaaagctgtc
     1381 ctaaacccga ctttggcgcg tttgcgctgc cgccatccat ggccgcgcaa accccggtgc
     1441 ctcgtgcacc agggcataaa agtaggggta caccccgaag agtaatgcgc gcctcgaggc
     1501 ttccaaaact atttgcgggt tcaattctgc aaatgacaac ggcgccccgg taccggcttt
     1561 ttcttatccc gcaacccgcg attctcagaa tcgcactcgg gctgcttcgc ggcagcaacg
     1621 cagaaaatcc gttgcaccga cagagggctc ttagcatgct ggtcgctctg accatgctgg
     1681 acgacctaaa agacgaagag ctgatcaggg aactcaaccg cgctgaactc gcggaacacc
     1741 tgaccggcga cgacgcaggt gcagaacgca tatttaccat acttgtcggg cggcacaacc
     1801 gcgcgtttgc caggctggta accctgcgtt acggtgtcga ttcggcgagc gccgatgaga
     1861 tcgcgcagga tttctggctc gaatgttact ctgccctgcc ccggtataat cccgagagac
     1921 ctttcctcgc ctgggcaaca acaatactct ttcgcaccgc cgagaaattc ctgcgtaaac
     1981 gcagccgcga gatacagctt tcaccgcaga gcgatttttt tgacatgcag gcctcgaatg
     2041 aaaccccgga aagcgaggcg atgacacgcg agcagatggc cgctatgctt tctgctctgc
     2101 gccagctgcc acctgaactg gccctgctga ttcaactgcg tttctttgag aacaagaaaa
     2161 tcgaggatat taccgccgca acgggacttg gccgctcgac gatctttgaa aagctaaacc
     2221 tggcctaccg caaaatgcgc aagctgatga aagagtaatg attagccagc ggtacgaggc
     2281 ctgtaccggg ccctccctgc ctgttacagc agctctttcg gtgctttttc cctgagttct
     2341 ttgatttcag gcagcgcgtc gcgtttagcg atcagcagca cgccgttatc ttcgaccagg
     2401 acacagttat cgagcccgag cagcgcaatc gtcaagcccg tcttgacgat gttgcctttt
     2461 gcacggtggc tgataacctt gccgctgatc acgtggttac ctgactcatt caccggatag
     2521 atacgcccga gcgcctcaaa cgaaccgaca tcgtcccaga gaaagtctgc ctgtatcagg
     2581 ccaactttct tggttttttc catgatggcg tagtcaatcg agatattctg cattttcgca
     2641 aaactttttg cgaggttagc aggggtctgt gcgcaggcat cggcaagttt cagcatctcg
     2701 gcctggtaga tacgcaggtc ttcacgcatg cggcccagcg aaaaaatgaa catgcccgag
     2761 ttccagtagt gccgctcggt tgtcaggtag tggctcgccg tctgatagtc gggcttttcg
     2821 cgaaagctcg aaattgcact catgcgcggg gccacttcga taccagcctc gatatagccg
     2881 taaccagtct cggcgcgcgt gggcttgatg cccatgcacc agatacgctc ccccgcgcgg
     2941 gtcagtgcca gcttcaccgt ctctgcccat ttttccgcgg gctcgatgtg atggtcggcc
     3001 gccaggatga cgacacccga cgcgtcatcg gcgccgtgct gtttcaaaaa tgacgtaaaa
     3061 tacgcgataa tgggcgccgt attacgggcc tctggctcga gcaggtactg tttcttgtcc
     3121 agcttaacga tcttctggat cttggccgcg aggtctttat ttgtaccgat gtaaatgcgg
     3181 tcgggcgttg tcagcaggcg cgcgcgctca acggcttctt caatcagcgt ctttttgccg
     3241 acgatgttga tgagctgctt cgggcgattc acgcggctca tgggccacag gcgggtgcca
     3301 gagccgccgg ccatgatcat aacgaacggc ttgccgttct gcgcgaattc ttttgtagct
     3361 tttacgggtt ttgccggttt agcgttgcct ttgggctccg ccgatttctc cgcctgcttc
     3421 gtttttgtct tcgttgccat tacgacctcg tgctgattcc ttttcgttcg gtggtatgat
     3481 ggatatacgt ttttctgtgc cgtgcgcgga cgcgtcgaga atgacctgcc ccgcgcgcgt
     3541 cgcgattttt tcaatcttgt agtattcgag taatgtcgga ttttcggcca tgagcgcgga
     3601 cattttttct gccttggcga ggtcttccat gttgcgcttg cgctccaccg agagttcggc
     3661 ttctttctcg atttgtgcca gcagggcgcg gcgatcagcg gccgcaacct gctccaggtt
     3721 acgggtctgc gcggcgtaga tctcgctgtc gggcacatgg atctcgcgca gctccacttt
     3781 cttcagccgg taccagggtg tgcgaagctg cgcgtcgagc cgcttttgca gttcgggcaa
     3841 attggcaccg ttgaaaaaag aggtcagctg gattttcagc cgttcgagct gccgttcatc
     3901 ggtattcagg ttaatgaagt actctgccgc gagaaactgt aagctttctt cgacaaaacg
     3961 atcgcgttcg gtggggcgca tctgcagtgc tgcgagcgca gccgttgcct gtgcctcgtc
     4021 gatttcaccc tcaatacgca ggcgcatatg aacataaaaa agatcgttga gcctcaggta
     4081 cgcgctgtaa cgcaggggca aacgtacacg cacttcctgc aggcgcggtg cagtttcgag
     4141 ctggtagcgc cgccaacggc cgggtacaaa cagcgttggt acaaaggtat aacctgaaga
     4201 aaaagcctgt aactcggtcg cggtggaaga actgccagtg gcgcgctgca ggaataccag
     4261 gtgccccacc ggaacagaaa gtacgtcaag gtagatgaca ctgcctaccg aggcaatggc
     4321 cagaatctgc agcgctctcc ggaaaaaatc gccgagcata ttgcgatggg gttattaacc
     4381 cccggctatt cttcctcggc gccggtttca ggattgtgca gcctgatggg gcgttcgggc
     4441 gaaatagtgg ttactgcatg cttgtagacc agggtctgct tgtggtcgtg ctcgaggacg
     4501 atcgtgaagt tgtcaaagct cacgacccgg cccttgatcg ggactccatt cgaaagatag
     4561 atggtaatcc ccatcttttc tttgcggatc tggttaagga taaaatcctg gaggttattt
     4621 ttcgtcattg gacacgtacc tgcatgaaat cccgggcaag cgcggcaatt agctcatgtt
     4681 cataagacgg gtcaactgtt tttgtaaatg gctgtttggc ggagctattg cggaaccagg
     4741 tcatttgccg cttaacgagc tgcatagtgt tggtgatgat gctctgctcc actgaggcca
     4801 gctgctcagt attcaactct gccggtaaaa gcccggtgct cgcgtaggcg tcgaaaaact
     4861 cactggtacc tatacctgtg agcgccggca gcgtgtgcca gtcgaggccc gcggcaagtg
     4921 cgctgcggac gacagcatct gcctcaccaa ccatgcccgc ggcaaacatc tgcttcaggc
     4981 ggaaacttac acgctcgcgc agaacttcgc gctgcggcac aaagacaagc atcagtattt
     5041 cgaattcatc ggcgatcccg ccggcgcgcg cgatgctgct gatcggacca ccgtgcgcca
     5101 ggcagagcat gagctggcgg ttcagccgta cccggttgtg ctggtgcacg cgtgcagctg
     5161 caacaggatc gatggcattg aatttttgcc atgcttctgc ctcggacatc tcttcgacct
     5221 gggcgcgaat cgtctcaggc acaggaatcg cgggcaacaa accatccagc aacgactgga
     5281 tgtagaaata agttcccccg cagaccacgg gaatcctgcc ttgagcaagt gagcgagcga
     5341 tcgcctggcg ggcatcggcc acgaatgtac cggccgaata gacagcatca aggggaagaa
     5401 agttgtagag ttcgtggtaa aacagtccga gctctgcgct gccgggtgag gcagagctga
     5461 tgcgcagatc acggtaaacc tggcgtgaat cagcgttgat gatggccaac ggcagatcgt
     5521 gcagcgattg gtaaaggaat gcagattttc cactggctgt cgtgccggtc agaatgatta
     5581 agcggggttt ctgcgccatg tgcgccggtt aaaccatgtg cttgcgcacg atagtcgcca
     5641 gctcttcagc ggctttcatc aggtcgtcgt tcacgaccac gtagtcgaaa ttgttttgct
     5701 ccatgacctc gagcctcgcc agctcgagcc ggctgcgtat ctgttcttcc gtttcactgg
     5761 cgcgggcacg cagccgccgc tccagttcgg tcatgctcgg cgggatgata aagatactca
     5821 ccatgcgcac gggctttccc ctcagggtct gcgctccctg gacatcgata tcgagaatcg
     5881 ggtatttatc ctgggcgaag attctcctga tttctcttat ttcggtaccg taataatttt
     5941 cgagtacgcg cgcgtattca agaaactccc cgccctcaac tcgtttctcg aattcttcgc
     6001 gcgaaagaaa atgatagttg atgccatcgg cctcgttggc gcggcgggcc cgcgtcgtag
     6061 acgaaatcga gtgcatcagg cgtggctctt tactgagcag gacattgatc aggctgttct
     6121 tacctgcgcc agacggtgcg gagatgacaa agattggacg tgagtccatt tctcttaaac
     6181 gctgctttgc ggtgacgcgt aaagaagaaa ggggttgcgt ggccccgaaa ggtaatctta
     6241 actcaaggcg agccggtccg cgtataccct gtgccaaagc gctcaagcca gcgtatccgt
     6301 tctttcgcct ggctgtggta aggactacca ggatacctga gaacctgctg gtaagcgaga
     6361 cgcgccttgt gcgtatcgcg gtgataccag gggcgctcat acaggctacc cagtgtatac
     6421 cacgcccggt cgaatggcac ctcgttcttg cgcgagggag ttttgggctc actggcgagt
     6481 aaccgcgtca ggtgtggcag agccgcttcg aacctgccct ggtcgagcag cagctggccg
     6541 aggcgaaaag ctgctaacct gccttcatgg gacgaaggtg ccagttcagc aaggttggca
     6601 tagacccgct cggcttctgc caggcattcg ggtttctgcc tcagctcgcg cgagcaggta
     6661 taataaccgt cagcaacccg cagcgatgcg gcaagcctga gcgcttcgtc caggttttcc
     6721 gcttcggcgg cgagtcctgt atcaccgcgc ttcgcgcgca gccagtacga ctgcagtcgc
     6781 gcctcgcgcg aatagttatc ttcgccggct tcctgtgacc ggcgcaggaa ctcgagggcc
     6841 ttgtcggtgc ggtcggaacg caggtagagc tgcgcgtttt ccaggtaaac ttttgtcagg
     6901 gggttacgca cctgttgctg cgcagtttct tcctcagcgg cattcgccgg ttctgcggtc
     6961 gcacttgctg catcagcccg cgacttttct ggcggagcag ctgcagcatc cggagtcttg
     7021 ccttcgacag gcacagcctg cacgctgcca gcctgtggtt cagttgtact ttccgcgagg
     7081 ggattaccgc gttcatcgta gcgcttacgc gcatccagag taccggcaat gaataatgcg
     7141 gcaaaaactg cgaagaggcc tggcagtctc attagtccat gaactccagc tttttgcgcg
     7201 catcgcccgt ggggccagcg gtgcgctctg tcgtgcgagg catttcagcc ggcctacgcg
     7261 cagcatcagt tgtcgcaggc ctgttgttac cctgcggacc atcaaaaata tcccgacctg
     7321 cgccctgcgt ggctgttcgc ggcgctggat tcactgctgc gccagcgggc gcctcgccct
     7381 gcaccaccct ttccggtggg cgcgagccaa tcttttcttt ggtcagcgga gcttcgtcgt
     7441 cttcaatcgt ctttttatcc ggtgtcaggc gtggtacagt gtaactgtca cccaggtaac
     7501 cgggggattt ctccttggga cgcagcgcat cttcggattt gagcatttct tctttttcac
     7561 ccatgaggtg tcccatggtt tcgcggccca tcgtcacgaa gcgcgtgtca tcggccgcct
     7621 gctggcgcga aggaaactgc agcggatcga cttcgacatc ctcgacgagc tgacgcttct
     7681 tggcatactc ttcgcgcatt tcctgcaggt aaaaaaggtc gcggttgcgc tccaccagct
     7741 tgtagccggc cggtttagca tcacgcagcc ccttgttgat gccggagata ataaccagca
     7801 gagcaatgag cgccgcgaaa gctatcgagg ccttctgaat aatatcccgg atatggggag
     7861 gaatgcgttc aatcagcttg tccagcgtgg gaataatatt accgagaatc cggcgcagca
     7921 taccactagt atcgtaaaaa agtgcgcagt gagagcgcaa tttttgcgga cttatggtag
     7981 cgagaccgca ggccccggtg tccccagagc ggccttatgc tttacactat gtgcgccgca
     8041 caaaatgtga catatgcaga ctctgaaaaa gaagctgcgg cgtccaaaat accggttttg
     8101 ggcggcgttt tcaacggcgg cgttgctgct attgctggaa gtcataccca aggcagctgc
     8161 ctatgcccgg gtctcacctt ctgctcccgt gctaccgcag gaagacctga ttcgcgacga
     8221 ggtagtgcgc caggcacaat cgtttctgaa tcttccttat aaattcggtg gcgcttcccc
     8281 taacggtttt gactgcggcg ggctggtact ctatctctac gggcggcacg gctttaccat
     8341 gccccacggc gtgatctaca tgaggcccac actccgcctt accaggaccc ccaagaaggg
     8401 cgatgtgatc ttcttcctga atgacaacaa tatcgtgggc catgtaggca tgtatatcga
     8461 cgaagaacgt ttcatccacg cccccaaaga gggcgaagtc atccgttacg aaaaactcaa
     8521 acatccttac tggaaaaagc gtacagtcga gattcgttct gtgttctgaa aacgtcttgc
     8581 ccgactgccg gtaccaaagg ggggagctcg ggcgttctgc tgcctgctcc ttattgagaa
     8641 tgaaatctca ccttaatgac atctctgtca tttttttggc cccgccattc cgtgccgcga
     8701 cataatcatg cgtacggcaa agctgtacaa ttaagacccg gcccacccag tgctctgaag
     8761 aagagccgcc aaacgggtaa ccgggttaca agtggaaagg agatccaatg aaaaagctca
     8821 tcagcatcgc catggtgctt ttgacagcct caagcgcatt cgcgtggaag gggcagaccc
     8881 acaaccgcat ttcacaggac gcaatcgacc tgctgaacag catgacgacc aacccgcagg
     8941 cgcaggccgc cgtgcagaaa ctggtttccc gctatggtag cctgacagcc gcgaaaagcg
     9001 ccttcggcaa ccaggcaaaa gacgaagata tcccgcaacc aaactttccc cttggcaacg
     9061 acatgtggtt tgatgcctgg tggtcgtact ctcgtaacgg ctcgccctcg ctgtttggcc
     9121 tcgtcgatgt cgcctactac gtggcgcttt cgcacttcgt caacgcgttc aacgcatcac
     9181 cttctgctga ttcacacaac cagaagccaa tgggctacaa ttactaccgt agccgcgcgc
     9241 tctacaacct cgtgggcgac aattttaccg gtgactatga ctgggtagca gaagtctaca
     9301 actacaacct gaacccgacc agcgacacta tttcacgcat gagaaacctg tgcggtggct
     9361 cctgccgttt ttcgaccggc gcctttgata aagattacca ggatgtgaac cacgtctcgg
     9421 gtgcaagcgc ctgcgcggcg ctgatcgacg gtccttcggg ctacgttgcc agcggtaacc
     9481 tcacgcttat cgcgcgttgc taccactaca ttgaagatgc atcacagcca caccacgctg
     9541 gtgcctacta cggtaaagac cacacagact ttgaaggttt cgtagaagac aactactaca
     9601 atccttcgta ccagattagc cagactgcgg cgaaactcgc cgaagcacag gctctctacg
     9661 cgcaatttgc caacatgagt gctgagaacg tggtaaagac aatggcgcag tggtctacga
     9721 ccaactgcta tgcagtctac accaacgact ataccagcaa cgaaattgcc tgcgccgaaa
     9781 aactgctgcc gcgcatccgc gccgctgtag ctgccgtcac agcgcagaca atcgcccgca
     9841 gaccgtaaca ggtgaaggcg aatttcctgg caatcgccgc gctctgcgcg gcggttgctg
     9901 tctcgtgtaa aggaaaggtc acttacgacc tcgaaccccc aggtattctg ctgcagagcg
     9961 gcagcttatc tcttccgaca aaagttttcc aatcgatgtt tgacgtgatg cgcaagtcgc
    10021 gtatcaaaga ggggcaagac ccccgtagcg ctatattcat ggtggttgca gaccattacc
    10081 tcgctgccga atacctcaag cggcactctg gcaaaccgga tgcccttatc ggcgcggaag
    10141 ccagcaaggt ttattacgag acgctggcct actacctcag cgaacgcaat ttaaaacaga
    10201 cttttatgaa atgggtgaaa aacaccacga taccgcaggt tcctgaactg cgcgcgctat
    10261 ttgacgacaa ggctgaagcc gcctatacca acataccgct gcgccgcgca caggccgaca
    10321 cactggtgct ggcgcgctac ggtgaagccg gcgataaaac gctcacctac tcgcggcttt
    10381 tcgattcttt accgcacgca ggccagctgc acctgtttac ttctcctgat cgccgctcac
    10441 tcgaagagct ggtggcggcg cagctaagac gtgaattttt tgacgaatac gtggcgaaag
    10501 caggtgaagg cgataaatcg gaatacctcg cgctcaggca gctggtcgag aacagcatct
    10561 actcccgtgc cctgcgctac gaaatgggta tggagaatgc caacccgcac gccgagaaca
    10621 gcgcggtgaa agaacgggca aaatccgttt ctttcagcaa ggtgaaagaa tactacgccg
    10681 ccaacagaga caagtacaaa gaagtgcaga ctgtcgactg ccgtcatata cagctgaagg
    10741 attacgacct ggccatcaac ctgcgcgaca agatcgacca gggaactgac atggtggcgc
    10801 tggtaaaaaa gcactcgctg gcagcggaca aaaatagccc ggaaccggga ctgatcaaag
    10861 gcataaagaa cgatactgaa ctgcataaaa gacctcgcct cgaaaccctc tgcatgatgc
    10921 ccaaacaggg tgagtcagag gtcgtgcgcg acggtgattt ttttcacgtc gtcaaggcgg
    10981 agcgccgcac cgatggatat cccccgctcg acgaaaccac gcacctgaaa gacgatattg
    11041 cccgtgaaat tgccgcacag gaactcaagg cagaatttga cgcacacaag aaaaaagtac
    11101 tcggacgtgt cgacattcgt atcaataaaa ccgaactgga gaaactgaga tgaaaaggta
    11161 tcactggatt gcactgcttc tactgggggc catcgcggtc tactggattg cacgtagtac
    11221 aggcgacaag aaacagacca ggacctcacg cgacgatgtg accgtcgtat cagcttcggg
    11281 ttcagcccac gacctgcagc cagatatgga aaactaccgc ttcgaacgcg agcagctgct
    11341 ggcaatgccg gatattgggc aggcccgcgt gaagccggaa atgaagaacg caccggaata
    11401 caaggcatcg cccctgcgcg gccgtgttgc tgcgggcccg gtcgacgact ttggcgcccc
    11461 gctctttata ccctcagaaa ccgcgatgac cgacccgaaa cttttcgagc aggagctcgc
    11521 gaaacaccac gaacgggccg acagttacgc tgccgcccgc atggatgagc tgcggatcaa
    11581 taccgaagtc gaaaagactg ccatgaagag taacatcgac caggcaaagg cacagggctc
    11641 ccggtcgccc gaagagatca aacgcgctga agaagcgctc gcccgcatgg aacagctgca
    11701 aaaagtcctg aacggcgaaa agatcgagaa ccgcctcgaa tagacctgtc cgggcaggca
    11761 attcgttcga tgtcggacta tttgaacccg acctattaat attttccgac tttttgcggc
    11821 ggacataatt tagccgcatg attcaaaaac tggaaatcag ccagcagctg cgcgatgcac
    11881 tgcaggcaga aacccgcagc gcgcagcatg ctacccgtga aatacgcatc cggtccacct
    11941 ggcccggcat acagctctac tacccgccca tcaagtacct gccgcgcgat aaccgttacg
    12001 aaaccgaaag cgaggcgcgc gaacgtctgc tcaagtacgc acgccagagc gaggcgcata
    12061 cgctcatttt tgaccttgaa gacggttgca ggcaaaaatc gctcagccgc cagtttttgg
    12121 caatgtcgtt acccgatgtc cgcgcggcga tacccgggcg cgtggccgtt cgcattaacc
    12181 cggtcacctc gcccgagcac tctctcgacc tgtcgctgct gaaggaaatt gccccgtacg
    12241 ttgacgatgt gatgctggcg aaggccggcg agcaacacgg tattgccgaa ctcgacaagc
    12301 tcggtgcctt tctcgatagc ctctcacctg ccatccgcat acagccgatc atcgaacatc
    12361 cgcgctcgct taaaatcgca ggcgaaattt ttgggcacca gaaagtcgaa cacgttgtct
    12421 tcggtattca cgatttttcg cgcgcgctgc agctgatgat caccgcagaa aactggcagg
    12481 atgagctcta cccgtttatg tgcaacctga tggttgaggc gcggcttgca ggcaaaggaa
    12541 tcatcggtgg agtggaaaca ctgctgcttt ccgcagaccc acccgacgta gcgctcatac
    12601 ccgaatggct gaaccgccac gccgaggcca atgcgacgat tatctgtaac catgcacgcc
    12661 gtgaagcgtc gctcgggttt accggcaaac aggtaataca tcccaatcac attcgcctgc
    12721 tgcgctacgg cttttcaccg ggcaacgaag acctgcaccg ttcgctgcgc attcttgaag
    12781 aggctatcaa ggccaatgca tttctcggtg gtgcgatacg ctttgaagac gaaatgatgg
    12841 atccgccaat gtttgccaag gcgctgcagc tgctaatgcg ctcggtgatg ctgggcaacc
    12901 aggataccgg ggtcatggaa gttgttgcag gcctcattgc gagtctgccc acggagaaat
    12961 tccccgaaat ctggccgtac cccggctggc cacctcttgc catacaggaa taataaataa
    13021 aaaacccctg gagtaaaaaa tgagaaacca agtacctgaa tttctgaata tcgctgaaga
    13081 atgcctggca aaacgcgccg ccggcaatga caagaacaag gtggcgatga tcttcgccaa
    13141 cgacaaggac gaggcgcgag agtacaccta cgcagaactc gacgatctgt cggctcgctt
    13201 cgcgcaatcg ctcaccgagc gcggcatcgc ccctggtgcc agggtcttgc tgcggttacc
    13261 caacacggtc gcatttcccg ttgcgttttt cggctcgatg cgcgcgggtg ctattcccgt
    13321 acccacctca cccatgctca ccgcgactga ggtgcgctac ctgatcacca actcgggcgc
    13381 cgatgccatt gtgaccgaag agaagctgtg gtctgctgtg gccgacgaaa taccggcgag
    13441 agaggtgaaa gcgctctacg tttcgggcct ttcttcaaca acaggagcta tcaacttcga
    13501 agacgaactc gccgcaaaaa agccggcggc taatattcac cgggcacgct ccgctgatgc
    13561 tgcctacctc gtctatacct cgggcacgac tggatatccc aaaggtgtgc tgcatgcaca
    13621 ccgcgcgttg atcggccgat tacctgctgc ggagaactgg ttccactttg ccggcgacga
    13681 ccgcattttg cactcgggta agttcaactg gacctatgtc ctgggtaccg cgatgatgga
    13741 cccgctctac cagggcaaaa ccgtgatcgt gtacgaaggc gaaaactccg ccgaccgctg
    13801 gatctcgctc atcaaaaagt tcggctgcaa catcttcatc ggtgtgccta ccatctttcg
    13861 tcagattctg caaaagacga cggcaaaagc tgaagatgtt cccacgctca ggcattgtat
    13921 gtgcgcgggt gaacacctga ccgacgaggt cctggacggc tggatcagcc ggtttggttt
    13981 tccgatctat gaaggcctgg ggatgtcgga gtgttcgtac tacatctcgc agaagaaagg
    14041 tgatgaaatt gtcaaaaact ctgcaggcaa aattcagccc ggccacctcg tgcaactgct
    14101 cgatgaagac ctgaaacctg tggctgcagg cgaagaaggt atgatctgca ttagtcgtaa
    14161 tgatccaggc ctttttgtcg aatactggcg cgcagcagag cagactgcag cacaattcaa
    14221 gggtgattgg tttttgacgg gcgattacgc gatacaggac aaaaacggct atatcttctt
    14281 tttgggccgg cgcgacgaga ttatcaagtc gttcggctac cgcgtgtctc cgttcgaaat
    14341 agagcgcgtc tatcgcgacc accccgcgct cgatgacatc gtcgcgtttg ctgaacacct
    14401 cggacctgaa aaaacgctga tctcgatgtg tgtccaggta aaaggcggca caacattcga
    14461 tgaacaggag ctacttacct ggggtaaaga acggctcgca tcgtataagc tgcccaaaaa
    14521 ggtctacagg ctggataaat tcccccggtc ggccaacggc aaggtactgc ggtcaaaaat
    14581 accaaaagag ctgggcctgg tctgaataac ctcttcttct tgactgcgta ccgccccgaa
    14641 caagcggtat tgcatggcga agaggtattc cggcagcccg gaagaaatac tgcaggttga
    14701 cgcctatgtc aaactgatgc gctgtgcaga cactgtccag ggcatcctgg cgcgcaatgc
    14761 cgaggccaac ggccttaccc tttcgcagct gggagtgctg gaaatcctgc tgcacctcgg
    14821 cccgcagaac cagaaggacc tcgcgggcaa gatactgcgc agcggtgcga acatgaccac
    14881 gatcatcgat aacctcgaga agcgtgaaat cgtacgccgg acacgcgatg aagcagaccg
    14941 gcggacgatt cgggttgagc tgacggaaac cggcgaagac ctgatcagcc gtattttccc
    15001 gctacacatg aataatatct accgtatgat gggagcactg gcccccgacg agctgcacgc
    15061 gctctcacag ctcacccgca aactgggtat cgccgcgcgc ggccgtatga ccgtttagca
    15121 gattactttt cgccgatcag gaactcattc gtgccgtctt cacgaatacg ctgcaccagt
    15181 ggaaactgca gctgccggcc ctgctcgctg agccgcgcaa tttcggtcca gaccttgcgc
    15241 aggccaaccg ccgtctgtgc gtaagtctca atgcgcacac tgcgggcaaa ctcaaccctg
    15301 tgcctctgcc ggccctgtcg cagcaggtct ttcagcaaat tttctggcaa ctctttggcc
    15361 agaatacagc cccctgctga acgcaggtag ggcttaccgg tcgttatggc attctcatag
    15421 tagaccagaa ccgggtcgca ggtaaaacca ttcgcctcga gcaactggcg cgtcttaacg
    15481 acctcggcgc cagtcttatt gtaatcacct gtcacctggc caccgatcac ggtcagatcg
    15541 agggcgctcg caggactgat ccgtacggca ctgaaaatac ccagatacca gtaggaaaaa
    15601 aggatgaagc ccggaaccgt cagcgtaaaa accgcgatca ccagccagaa ttttttgttt
    15661 ttcatttgcg cgccaaagct cccaaccatt gatcaatcgc tttaatttct gcggcagaca
    15721 gcgcaatcac cgaaagccgt gtatgcgtaa aaagctgcga gctgccgagg ctggtatgca
    15781 ccatctgttc acgcgtaaac gtcgcggggt actttttgac gaatttcatt ttcggcgcga
    15841 accagcgcgg cttttcaggc gtgctcctgg cgtcaaaata aggcgaagcc gcatcgaact
    15901 gcgcagggtc gggcatggct gcagaggcaa cgaccatttc accgacaata ccgggcaccg
    15961 ggcaactgct gtggtaaaaa aaagcccggt cgcccttctg catcgctacc aggttattgc
    16021 gtgccatgta gttacgaacg ccatcccaga gcgtggtttt ctgcggcgat gcctgcaggt
    16081 tatcaatgct gaaggtctcg ggttctgact tgagcaacca gccagcaggt ttcatgcctg
    16141 ctctgaaccc atatcccaga tatattcaaa ttcttctttt gccgcctcgt cgaggcgctc
    16201 gggctgaaag agggtcttca tttttggact gggataaagt ttaatttcgt tggtttcagg
    16261 aatgtgcgtg agcatccgca cgatcgtgtt ctcgccttcg atttcataga gcatgaaggc
    16321 cgttcctttg ccgggtaccg gcatcgcgga tttcaggtag cgcatggttc tgtttattta
    16381 ttttcgctgt cgcgaaaata aataaaccta gcttcccgcg acggtcaggc tttcaaagaa
    16441 gacacccctg gggcctgaat acgcgccgcg ctggacgcgc tccgctgatt tgagcgcacg
    16501 cgcaaaagcg gtctgtgtac tgcccgacac cgaaccacct tttatcggta ttttcttacc
    16561 gtccttgtgc aggtaaccaa ggcgaatttc ccctgaaaat gcgccggaaa tcgtgtttgg
    16621 ctggaatgtc gaaaatttcg tcagttcaag caccccgttt tgcagaaaat ctgcataggg
    16681 agtcgtacca caatctacct cgatgttcat gagcgcgctc gtgcggggta aacggagcag
    16741 gtcagcataa cggccatcaa tcaggaaatt cttcacgcgg gagttctcaa tcagctgtac
    16801 acgactcgtt gggaaaccca gctcgtcaaa actatacgag tgcatacccc ccgcgagttc
    16861 gggtgtcgag gcaatgttca gcggttcgcg ttcattctgc agcacggcat caccggcaga
    16921 aaataccgaa tagcggttga agagtgcatg cccgtcgagc tgggcaacaa aaaagtcgaa
    16981 taacgaatcg agtgcgtcac cggcgagcag taccggcagt tcacccgtag gtggtaactg
    17041 cgcaacctgc aggtctgcga gtaccgccgc gtcttctgcc agctgctctt tcagagcgag
    17101 gtctttcagg aaacgccggc gcttgatacc cataatctcg tgccggttat cgggcgaggt
    17161 cagcacataa tcgcagacca cgcgtgttga gtcggcacgc acagaaagac cgtggtgatt
    17221 ttcaatcgta taggcaaagt cttcgaggaa caattcgaga ctgtttaaag gaaatttttt
    17281 cagttccttg ctgtgaaatt cctggatatc agtgccgaac gaaaccagtt cgtcgagagt
    17341 ggcatcactc accgagtggt caaatacctg tactgcctcg cgcggactcc agggcgctga
    17401 aaagccaaaa cagggatttc tgaccagcc
//
LOCUS       DAOQJF010000017        17237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1569_length_17237_cov_5.358631, whole genome shotgun sequence.
ACCESSION   DAOQJF010000017 DAOQJF010000000
VERSION     DAOQJF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 17237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17237
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1569_length_17237_cov_5.358631"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..521)
                     /locus_tag="PLF85_01800"
     CDS             complement(78..521)
                     /locus_tag="PLF85_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNE18418.1"
                     /translation="METIVATTGLALMVGLSGIGSAIGLSMAGSSVLGMIKKKPEAFG
                     NALVLSGLPATQGLYGFVAFILYSGDVKTGITMFHAAVVFGAGLAVGLVCFISAIQQA
                     NVCSSGIQAIGGGHDAFGKTMILAAFPEFYAILSLVAAILMKGLL"
     gene            complement(532..2445)
                     /locus_tag="PLF85_01805"
     CDS             complement(532..2445)
                     /locus_tag="PLF85_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNE18419.1"
                     /translation="MIEKMHRLSLLLFYTEKKRILRGLQELGVVDLDLAQSESPRIRR
                     AAQDANELKKSLGLIEKVRKQLPQGAELPKIEMPHESGAQLELVRALLSEKEHLENAV
                     RELADEYARYSQWGEIPVDGIAMLAQKGIGIHLFSGSEKFFDRYDFGGHCVEVISRGG
                     NTVRFALVTYHEDAPVIPFQKYAVPGRSLSEMQAERQYLGKKLNLVLAKIAALSSGAG
                     RLQNSAAHLETRRLFETAKHSLEADKTGSVFSITGYFPESSAERVKRFLESHSLAYEI
                     SQPAADDQVPVRLKNNWLSRLFEPVTQIFSLPDYRELDPTPLFAPFFTLFFGFCMGDV
                     GYGFLLLLVSFVLMSRRSLRGIGLLVLILSAATIVSGVLMNSFFGANLFVRDGQGLIV
                     ADKDPAIFAAYTVQGKTVFPAMTLSLLVGCAQIFIAFVLQSLNETLVLGWRYGIKAAA
                     MLLMAVSAFILGAHADFLGLGFNSAFAIGPLQVGWYLTAVPVVAAQVMLALAFVLFFF
                     VANPDRKIWLRPLAALWDFYGFSTGLLGDFLSYIRLFALALAGGLLGNAFNQIAFMLL
                     PKNATGYDFASPWLLGTILILIVGHGLNFALGALGAFVHPLRLTFVEFYKNINFRGGG
                     RPYRPFRRVSSAT"
     gene            complement(2442..3032)
                     /locus_tag="PLF85_01810"
     CDS             complement(2442..3032)
                     /locus_tag="PLF85_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNE18420.1"
                     /translation="MKYQLNKPALQKLRRDLAIRVRALPILKAKETALRLEVIKSQAL
                     QAKLEAEAAELYRTNEHFLELWTEYPGLLRIDEIHLSERSVAGVPVSIISEIRFHVLK
                     AGLFHQRGWVVKGTEILKEMVRLRIETDLVRKNTEVLQQARKKATQKVNLYEKVQIPQ
                     YTNAIRRIRSFLDDKENISRAAQKIVKERARQAVGS"
     gene            complement(3029..4342)
                     /locus_tag="PLF85_01815"
     CDS             complement(3029..4342)
                     /locus_tag="PLF85_01815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNE18421.1"
                     /translation="MKKVYTRLTRITKATITLNAEGVGNEEMAIVDGRRAQVVKISGT
                     EVTLQVFAGTDGLATNSRVHFLGVPPQLTVGADLSGRYLNSYGEPIDGGPALTGIPVE
                     IGGPTVNPVKRQQPFQMIATGIAGIDLNNSLVAGQKIPFFADPDQPYNQVMATVALRA
                     DADRIILGGIGLTNDDYLYYRNVFANAGVESRITAFINTTDNPPVERLLVPDMCLTAA
                     EYFAVERKEKVLVLLSDMTLFADALSIVSNKMDQIPSKDSMPGSLYSDLARIYEKAAQ
                     FPDGGSITIIAVTTLNDGDITHAIPDNTGYITEGQLFLRRDTTVGKIIVDPFRSLSRL
                     KQIVIGKKTREDHPQVMNALVRLFADAANAKTKQENGFDLSRYDVRALDFARDYSREL
                     LAIDVNISVEQMLDTGWRLMSTYFDKSELGIKQAILDKFWKSAPK"
     gene            complement(4339..6120)
                     /locus_tag="PLF85_01820"
     CDS             complement(4339..6120)
                     /locus_tag="PLF85_01820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNE18422.1"
                     /translation="MENKTQTRGKVTGVLSNLVSIEADGPVGQNEICYIELGGVRLMA
                     EVIRVAGNVAQAQVFESTRGIRSGDNVEFTGHMLEISLGPGILSRKYDGLQNDLDRLT
                     GLYLSRGQQVDPLDTQALYDFTPLVSAGAAVESGDWLGEVPEGFLSHKIMVPFALAGS
                     HKVESIVSAGKYRVDDTIAYLTNSAGEKIKVTMRQKWPVKRAITCYREKPRPFRLLET
                     GIRAVDTLNPMPEGGSGFIPGPFGCGKTVLQHAMSKNANADLIIITACGERANEVVEI
                     FKEFPLLDDPRTGRKLMERTTIVCNTSNMPVAAREASVYTGMTIAEYYRAMGLKVLLL
                     ADSTSRWAQALREMSNRLEELPGPDAFPMDLSAVISAFYARAGYVVLRNGKTGSVTFI
                     GTVSPAGGNMNEPVTESTRKAARCYYALAQKRADSKRYPAIDPLDSYSKYLDYPEFRE
                     HLANELMPGWADLVERMRDYLRRSVEVSDQISILGDDSVPISYHVTLWKGELIDFIIL
                     QQDAFDSIDQNTPMERQKYMLKLVADICEQEFSFSHFEDVSVYFKKLINIMKQMNYSA
                     FQDSSFAKYEAELRALLESKRPAGVSK"
     gene            complement(6122..6970)
                     /locus_tag="PLF85_01825"
     CDS             complement(6122..6970)
                     /locus_tag="PLF85_01825"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNE18423.1"
                     /translation="MAETNFHFLVNALPDVASLAADEIDEVRYLIESNLDHESLHTFR
                     FLLYRNDNKNLLKLMRKRDGILPKMADRFNTPAVFSFEDLDDMLIDAYEDEAEIPGYL
                     AEFLQEERQSNWAVRERENRLVDLYYEAGTCYPQDFISAMFLFKRDLKNILLALNARM
                     QGFKVARITVGDYDLPAALASSPQADFGLAGVYEYIPQMAAYLNEGRLVELEHLLDDL
                     LLDHCATLVGDSIFTLNYVLYYFLELSLRHRWHELTPEKGARALSETVQEIIRTAGQP
                     SKAEMN"
     gene            complement(6963..7562)
                     /locus_tag="PLF85_01830"
     CDS             complement(6963..7562)
                     /locus_tag="PLF85_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18424.1"
                     /translation="MQNTLERMVDRIYQEGISKAEDRARQIVSAAEARAEEITGKARS
                     EAEEILRRAKAEADKLLKTAESDLRLLGSRGLSQLKSDIGRLITARAVAQPVATAASD
                     VTFISEVITQLLRTGGGGSFSLVVPEALREQITAQLSARLGSGLNGLEIKSGTVRSGF
                     TVVQKEAGYELDFSEAALMEFLEPYLKPAVAALFREKNG"
     gene            complement(7682..9121)
                     /locus_tag="PLF85_01835"
     CDS             complement(7682..9121)
                     /locus_tag="PLF85_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE18425.1"
                     /translation="MMHGRLLFIIACLFAAAGILAQARPAEQAKTVVLEDVLGNIEKT
                     FPVILLATREIRKAEFDILAAQGAFDLALRGSVNNTTGYYNTNSAEMNIVKPTPIWGT
                     SFFAGYRVSNGDFPDYYTPRRTTSGGEIRFGGRIPLWRDRSIDSDRLELQQSQINKNI
                     AGNVLDEQRLGVYRDAAAGYWNWVAAGKRKRIVQDLLKIAELRKDQLQRRVKLGDIPA
                     IEEKENERAILARKEQIAAADRLLQKSALYLSLYFREVDGKMIVPVTGQLPPEFPVEK
                     PLAKETFEADKAFALKHRPELKRIESEIELEQNALRFHENQRGSQIDLIVAGSRSVQD
                     QQAQGALRRDPWEAEFGVVFNVPLQTRKQDGKIGGTEQKIEILRQKHSYQKDKIVIEA
                     NDALIALENSLQRLDIIRQELVVAENLAKAEKSRFDLGDSTLLIVNLREQAAAEVALR
                     MVSAELDYWVATADYEAVLMKYLKRPEKP"
     gene            complement(9118..11343)
                     /locus_tag="PLF85_01840"
     CDS             complement(9118..11343)
                     /locus_tag="PLF85_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNE18426.1"
                     /translation="MAAKAETLIFELRRLLQLERREFIRIIGFGIVQGLLALIVPIAV
                     GNLINSVSFGTLVQPVVVLTILVLVFLVASAVIRLLQFWFIEILQRRLYARVLIASAD
                     SSRVTKADERDKMNYFMEVFTLQKNLVSLLTDGSTALIGIVIGMAVISLYHPYFIYYN
                     LVLILIGGYLIGYTLFKEGFLPSFAESDSKYRMLAWLQGNIGRKKPSEEDAESLGRDY
                     LEKRSLLFRVLFRQHISLAALFVLGNGIVLILGGLLVLRKEMSLGQLVAAEIIVSHML
                     NTISRFGKYFENFYRISVSLVKLHQLGPEQTHRELEAYEGQASRSFTDYLHPHWQRMN
                     FRWVFIAAGIVLLTILFLPWQQTSYGEGRVVAFSPTDRQQTIEAPVSGRIVHWHVHEG
                     SLVKKGDRLVTISDLDPQLTERLEQERAALTDRMRAARNRVESIESRVTSLQLARSEA
                     VSAAENRVAMASERVRQSQQTVAAAEAALETAKLNYERQKSLMEQGLTSQRNVELAEL
                     ELKRTRTEEQRAVAGLKAAEEEKKAMQRDARRLARDADAAINDARATVQSARSELARS
                     NEDLPRVEQRLSRQKTQEVLAPKDGIITRLLVSHEAEFVREGKGLCILIPESGRRAVE
                     LWLEGNDITLVRDGREARIMFQGWPALQISGWPEGAFGTFPARVKFVDNADDGHGKFR
                     VLLIPDEKPDEPWPDTLMLRQGIRARGWVFLNRVSIGYELWRKFNDFPPEIPVDMRSQ
                     K"
     gene            11675..12523
                     /locus_tag="PLF85_01845"
     CDS             11675..12523
                     /locus_tag="PLF85_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNE18427.1"
                     /translation="MIINHNMSAINTHRVLKFQNWNLDKDMEKLSSGMRINRGGDDAS
                     GLAVSEKMRTQVHGLRRAEQNTEDGMSFIQTTEGYLQETAELVQRIRVLAVQAANGIY
                     TDEDRQQIQVEISELIDEIDRIASQAEFNKMKLLTGSFARLNPTASMWFHMGANMHQR
                     ERVFIETMTSAALKLRNPTVLTFVSISTPGKANSVIGLSDEALRIISKQRADLGAYYN
                     RLEHAAKGLMNAYENIQASESRIRDTDMAETMSSYTRYQILTQAGTAMLAQANQRPQS
                     VLQLLR"
     gene            12678..13517
                     /locus_tag="PLF85_01850"
     CDS             12678..13517
                     /locus_tag="PLF85_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18428.1"
                     /translation="MEIYVGDERLQFELTETLPFSGIMQKLQAWAESQNLYIVDYRVA
                     AKPGFENKEDLTSDEIAVINLQLGDQRELIESNLRELIDYTDRAGFHLAGVIQSGKIL
                     STQEEQDLTTGGVFLAESLQTLQTYLRPDNPESLARAVKELNSHPDLIEKINALALIQ
                     NQLKIWLRQTEFSRVSPEEATERVARFREQVPGMQEELEKIAARFTQGREQEALQMLE
                     TISQTLVDAVVLMRIANDKSQGAGEKLVDLLGQLTGAIDSRDLVTAADIVDFDLRDLL
                     KEL"
     gene            complement(13576..14388)
                     /gene="murI"
                     /locus_tag="PLF85_01855"
     CDS             complement(13576..14388)
                     /gene="murI"
                     /locus_tag="PLF85_01855"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNE18429.1"
                     /translation="MAQNEKSIGVFDSGLGGLTVLREIHRQLPGENLVYFGDTARVPY
                     GNKSAPTIIRYSREIVRFLLSHDIKAIVVACNTASSYALDAIREMTDIPVLGVIDPAV
                     RTLTQRAAPGSVAGLIATKGTIASNSYHSALERNHGRQKLISQACPLLVPLIEEGYAG
                     SKAAELILHDYLDPLAGQGAEYLILGCTHYPLIAPTISRMFPRFKLIDSAEETARELK
                     QLLGERDLLRRQADARVDLFVSDMTDSMQKLRQLFFAEHVDRFEVAQVGDDH"
     gene            complement(14423..15646)
                     /locus_tag="PLF85_01860"
     CDS             complement(14423..15646)
                     /locus_tag="PLF85_01860"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNE18430.1"
                     /translation="MSKILVQKFGGTSVGDPERIRRVANRIKSYYEKGYQLVVVVSAM
                     GDTTDELIDLAGKISTNPPKREMDMLLSTGEQISIALLAMALHEVQVPARSFTGGQVN
                     IVTDGNFSNARIESIDTKRLSSHLDQGHVCIVAGFQGIDQDQNITTLGRGGSDTTAVA
                     LAAALKAKECEIYTDVDGVYTTDPNKVEGARKLKQISYDEMLELASLGAGVLHSRSVE
                     FAKKYGVIIHVKSSFNYNEGTKVVSENEIMEKITVTGVTLKSDDARMTVADIPDKPGI
                     AAEIFQVLADKKINIDVIVQSTGKDKTNTIAFTLPVAAVGEARQTLEQLIKKWGAGSI
                     SADENIAIVSAVGVGMKSHTGVAAGMFKALADNNINIEMISTSEIKISCVIAKDRGKD
                     ALRLVHAVFGLDKVP"
     gene            complement(15692..16879)
                     /gene="purT"
                     /locus_tag="PLF85_01865"
     CDS             complement(15692..16879)
                     /gene="purT"
                     /locus_tag="PLF85_01865"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNE18431.1"
                     /translation="MPLSIGTPQKQGATRVMLLGSGELGREVVIELMRLGCEVIAVDR
                     YEGAPAQQVAHRAYTIPMLDGDKLRALIKEVNPHFIVPEIEAIATEVLAEFESAGVRV
                     VPTARAAQLTMNREGIRRLAAEELKIRTSPYFFVESLAELKTAADKLGFPFVLKPIMS
                     SSGKGQSVVKTPADIEPAFQYALEGGRGKSGRMIAEGFIDFDYEITLLTVRHTVGTSF
                     CEPIGHRQVHGDYHESWQPHPMSAAALSESKRIAEAVTGALGGFGIFGVELFIKGDTV
                     WFSEVSPRPHDTGMVTMISQNYSEFALHARALLGLPLPEIQILAPSASYVVLGEGKSQ
                     NLSYTGLEAALSDPQVSLRLFSKPEIDGERRLGVVLATGKNTDDARQRAGSAMEKVKI
                     IYG"
     gene            16991..>17237
                     /locus_tag="PLF85_01870"
     CDS             16991..>17237
                     /locus_tag="PLF85_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE18432.1"
                     /translation="MVKTSAYRWIILILFMLPAAMSQMLWLNFAPLLTAMQQKYKVDE
                     LTSNLLIMVFPLVYVLVSIPAGFLTDRRGYRYAITLGS"
BASE COUNT         3904 a         4925 c         4526 g         3882 t
ORIGIN      
        1 gcgtcgctga cttttcgctc tgtcaagaat tatgtcacat caccgggacg attggccccg
       61 cggggcaatc gtataaatta cagaagtcct ttcatcagaa tggccgcaac cagcgaaaga
      121 atcgcgtaga actcgggaaa ggccgcgaga atcatggttt tgccgaaagc gtcatgacct
      181 ccccctatcg cctgtatacc actcgaacag acattcgcct gctgtattgc agagatgaaa
      241 cagactagcc cgactgccag ccctgcccca aaaacgactg cagcatgaaa catggtgata
      301 ccggtcttca catcgcccga gtaaagtatg aaggccacga atccgtaaag accctgtgtt
      361 gcgggcaagc ccgaaagcac gagtgcattg ccgaatgcct cgggcttctt tttgatcatg
      421 ccgagcaccg atgatcccgc catcgacaga ccaattgctg accctatgcc cgaaagaccc
      481 accatcagag ccagacctgt tgttgccact attgtttcca tattcgtctc cttatgttgc
      541 cgatgacact cgccgaaacg gacggtacgg cctgccgccc ccgcgaaaat ttatattctt
      601 ataaaactcc acaaacgtga ggcgcagcgg atgcacgaat gcaccgagcg ccccgagggc
      661 aaaattcaac ccgtgcccga caatcagaat cagtattgtg cccagcagcc acggagaagc
      721 gaaatcatat cccgtggcat ttttgggcag cagcatgaag gcaatctggt tgaacgcgtt
      781 gcccaaaagg ccgccggcta gcgccagcgc aaaaagcctg atgtaggaga ggaaatcccc
      841 cagcaagcct gtggaaaaac cataaaaatc ccacagggcc gcgagtggtc gcagccagat
      901 tttcctgtcg ggatttgcca caaagaaaaa cagcacaaac gcaagggcaa gcatgacctg
      961 cgctgcgact acgggcacag ccgtcaggta ccagccaacc tgcaaagggc caatcgcaaa
     1021 ggcggagtta aaaccgagac ctagaaaatc agcgtgcgca ccaaggataa acgcactgac
     1081 agccatgagc agcatcgccg ctgccttaat gccataacgc cagcccagca cgagcgtctc
     1141 gttgagagat tgcagtacga acgcgataaa aatctgcgcg caacccacaa gcagcgaaag
     1201 tgtcatcgca ggaaaaaccg ttttgccctg aacagtgtag gcggcaaaga tggccgggtc
     1261 cttatcggct acaatcaggc cctggccatc ccggacaaac aggttcgcgc cgaaaaaaga
     1321 attcatcagc acaccactca caattgtcgc cgcagataaa atcagcacca gcaggccgat
     1381 gccgcgcagg ctgcgccggc tcatgagcac aaacgaaacg agtaacagca gaaaaccata
     1441 gccgacatca cccatgcaaa aaccgaaaaa gagcgtaaaa aatggcgcaa acagtggcgt
     1501 cgggtcgagc tcgcggtaat cgggcagcga gaaaatttgt gtgactggtt caaagagtcg
     1561 gcttaaccag ttgttcttca ggcgcaccgg cacctggtca tctgccgccg gctgggaaat
     1621 ttcgtacgcc agcgaatgcg actcaagaaa gcgcttcacg cgttcggcgc tgctttccgg
     1681 aaaatagccg gtaatactga aaacgctacc tgttttatct gcttcgaggc tgtgctttgc
     1741 tgtttcaaac aggcgccgtg tttcgaggtg agcagcagaa ttctgcaacc ggccggcgcc
     1801 tgaagaaagc gcagcaatct tcgcgagcac caggttcagc ttcttcccga ggtactgccg
     1861 ctctgcctgc atttctgaca gactgcgacc cggcacagcg tacttctgga acggaatgac
     1921 cggcgcatct tcgtggtagg tgaccagggc aaatcgcacg gtattgcccc cccgggaaat
     1981 cacttccaca cagtggccgc caaaatcata gcggtcaaaa aacttttccg atccggaaaa
     2041 caggtgtatg cctatgcctt tttgtgcgag catcgcgata ccatcgaccg gaatttcacc
     2101 ccattggctg tagcgggcat actcgtctgc cagttcgcgt actgcgtttt ccagatgctc
     2161 tttttcgctg agcagtgccc gtaccagttc gagctgtgcg ccggattcgt gcggcatttc
     2221 gattttcggc aactcggcgc cttgcggcag ctgcttgcgg actttttcga tcaggccaag
     2281 gcttttcttc agttcgttgg cgtcctgcgc cgcccgcctg attcgcggtg attcgctctg
     2341 cgccaagtct agatcaacta ctccgagttc ctgcaggccg cgcaagatac gcttcttttc
     2401 ggtatagaag agcagcagcg aaaggcggtg cattttctcg atcatgaccc caccgcctgg
     2461 cgtgcacgct ctttgacaat cttctgcgcg gcgcgcgaaa tattttcttt atcatcgaga
     2521 aaactgcgga tacgtctgat agcattcgtg tactgcggaa tctgcacttt ctcgtacagg
     2581 ttcaccttct gcgtggcttt ttttcgcgcc tgctgcaaca cttcagtatt cttgcgcaca
     2641 agatcagtct caatccgcag acgaaccatt tcctttagaa tctctgtgcc cttcaccacc
     2701 cagccgcgct ggtgaaaaag tcctgctttc agcacatgga agcgaatctc gctgataatg
     2761 ctgacgggca cacccgccac gctgcgctct gaaaggtgta tttcgtcgat acgcagcagg
     2821 ccggggtatt cagtccataa ttcaagaaaa tgttcgttgg tacgataaag ctccgccgct
     2881 tcggcctcaa gttttgcctg cagcgcctgg ctcttgatga cttcgaggcg taacgctgtt
     2941 tcttttgctt tcaaaatcgg caaagcgcgc acacgaatcg cgagatcgcg tcgtagcttc
     3001 tgcagggccg gtttattcag ctggtacttc atttcggcgc gcttttccaa aacttgtcga
     3061 gaattgcctg cttgatgccc agttcactct tgtcaaagta ggtcgacatc aggcgccagc
     3121 cggtgtcgag catctgctcg accgaaatat tcacgtcgat cgccagaagc tccctcgaat
     3181 aatcgcgggc aaaatcgagc gcgcgcacat cgtagcgcga caggtcgaag ccgttttcct
     3241 gcttggtctt ggcatttgca gcatcggcaa aaaggcgaac gagcgcattc atcacctgcg
     3301 gatgatcttc gcgggtcttc ttgccgatca caatctgctt gaggcgtgac agcgagcgga
     3361 agggatcgac aataattttg ccgacggttg tgtcgcgccg caggaagagc tggccttcgg
     3421 tgatgtaacc cgtgttatct ggtatcgcat gagtaatatc accgtcattg agcgtcgtta
     3481 ccgcaataat ggtgatagat ccgccatcgg gaaactgcgc agccttctca taaatgcgcg
     3541 ccaggtcgct gtagagcgaa cccggcatcg aatcttttga cggaatctgg tccatcttgt
     3601 tggaaacgat cgaaagcgca tcggcgaaaa gcgtcatgtc agagagcagc accagcacct
     3661 tctctttgcg ctcgacggca aaatattcgg ctgcggtcag gcacatgtcg ggcacgagca
     3721 ggcgctcaac gggagggttg tcggtggtgt tgataaacgc tgtgatgcgg ctttccacac
     3781 cggcattggc aaacacgttg cggtagtaaa ggtagtcatc gttcgtaagc ccgatacctc
     3841 ccagaatgat gcgatctgca tcggcgcgca gtgcaaccgt tgccatgacc tggttataag
     3901 gctggtcggg atcggcaaag aatggaatct tctgccccgc aacgagggag ttgttgaggt
     3961 cgatgcccgc gatacccgtc gcgatcatct ggaacggctg ctgccgcttt accgggttca
     4021 ccgtcggccc gccgatttcc accgggatgc cggttaaagc cggcccaccg tcgatcggct
     4081 cgccgtagga attgaggtaa cgccccgaaa gatcggcccc cacggtaagc tgcggtggca
     4141 cgcccaaaaa gtgcacacgt gaattcgtcg caagcccgtc ggtgccggca aagacctgca
     4201 gcgtcacttc ggtgccgctg atcttcacga cctgcgcccg cctgccgtcg acaatcgcca
     4261 tctcttcgtt gcctacacct tcggcgttca gcgtgatcgt tgccttggtg atgcgggtaa
     4321 gccgggtata aacttttttc atttacttac ccccgctggc cttttgctct cgagcagcgc
     4381 cctgagctcc gcctcatatt tggcgaacga tgaatcctgg aatgcagaat agttcatctg
     4441 tttcatgata ttgatcagct tcttgaagta cacgctgacg tcttcaaaat gcgagaaaga
     4501 aaattcctgt tcgcagatat cagcgaccag cttgagcatg tacttctgcc gctccatggg
     4561 agtattctgg tcaatgctgt caaaggcatc ctgctgcaga ataatgaagt caatgagctc
     4621 tcccttccag agcgtgacgt ggtagctgat gggcacgcta tcgtcgccga gaatcgatat
     4681 ctggtcgctg acctcgacgc tgcgccgcag gtagtcgcgc atgcgctcga ccaggtctgc
     4741 ccagccaggc atcagctcat ttgccaggtg ctcgcggaat tcgggatagt ccaggtattt
     4801 cgaatacgag tcgagtgggt caatcgccgg gtagcgcttc gaatccgccc gcttttgcgc
     4861 cagtgcataa tagcagcgcg ccgccttgcg cgtactttcg gttaccggct cgttcatatt
     4921 tccgccggca ggagaaaccg taccgatgaa agtcaccgaa ccagttttgc cattgcgcag
     4981 caccacgtaa cccgcgcgcg cgtaaaacgc agaaatgaca gccgagaggt ccatcgggaa
     5041 agcgtcgggc cccggcagct cttcgagccg gttcgacatt tcgcgcaatg cctgcgccca
     5101 acgagaggtt gaatcggcaa gcagcagcac tttgagaccc atcgcgcggt agtactcggc
     5161 gatagtcata cccgtgtata ctgaagcttc gcgcgccgca accggcatgt tcgaggtatt
     5221 gcagacaatc gtcgtgcgct ccatcagctt gcgcccggtg cgcgggtcgt cgagcagagg
     5281 gaactccttg aaaatttcaa caacctcgtt cgcgcgttcg ccgcaagcag taatgatgat
     5341 caggtctgcg ttggcattct tgctcatcgc gtgctgtaat actgttttgc cacagccgaa
     5401 tggcccggga ataaacccgc tgccaccttc gggcatcggg ttcagcgtat cgaccgcgcg
     5461 gataccggtt tcgagcaggc gaaacggcct gggcttttcg cggtagcagg taatcgcgcg
     5521 ctttaccggc cacttttgcc gcatggtcac tttgattttt tcaccagcgg agttcgtaag
     5581 gtacgcgatg gtatcgtcga cgcggtattt tcctgcactg acgatacttt ctactttgtg
     5641 gcttccagcc agcgcaaacg gcaccataat cttgtgcgag agaaatccct cgggcacctc
     5701 gcccagccag tcacccgatt cgacagcagc tcccgcagag accagcggag taaaatcgta
     5761 taacgcctgg gtatccagcg ggtctacctg ctgtcccctg ctcaggtaga ggcctgtcag
     5821 gcggtcgagg tcattctgca ggccgtcgta tttgcgcgac agtatgcccg gcccaagcga
     5881 tatttcgagc atatgccccg taaactcaac gttatctccg ctgcgaatac cccgcgtgga
     5941 ttcaaagacc tgtgcctgcg cgacgttacc cgcaacgcga atcacctctg ccatcagcct
     6001 gacgccaccc aactcgatat agcagatttc gttctggcca accggcccat cggcctcgat
     6061 actcacaagg ttagagagca cacccgtgac ttttccccgt gtctgcgttt tattttccat
     6121 atcaattcat ctccgctttg gaaggctgcc cggccgtgcg aataatttcc tgcacagtct
     6181 ctgacagtgc gcgtgcgcct ttttccgggg ttagctcatg ccacctgtgc cgcagactga
     6241 gctcgagaaa atagtagagt acatagttca gggtaaaaat gctgtcaccc accagcgttg
     6301 cgcagtgatc gagcaacagg tcatcgagca ggtgttcgag ttcaacgagc cggccttcgt
     6361 tgagatatgc ggccatttgc ggaatgtact cgtacacgcc agccaggcca aagtcggcct
     6421 ggggtgagct tgccagcgca gctggtaagt catagtcgcc tacggtaatc cgcgctacct
     6481 taaagccctg catgcgcgcg ttgagcgcga gcaggatgtt tttcagatca cgcttaaaca
     6541 ggaacatagc ggaaataaaa tcctgcgggt agcaggtgcc tgcttcgtag tacagatcaa
     6601 ccagccggtt ctcgcgctcg cgcaccgccc agtttgactg ccgctcttcc tgcaggaatt
     6661 cagcgaggta accggggatc tctgcttcat cttcataagc atcgatcagc atgtcgtcga
     6721 ggtcctcaaa agagaacacg gcgggtgtgt taaaccggtc tgccattttc ggcagaatgc
     6781 cgtcgcgctt gcgcatcagc ttcagcaggt ttttgttgtc gttacggtac agcagaaaac
     6841 gaaaggtgtg gagagattca tgatcaaggt ttgactcgat taaatagcgc acttcatcaa
     6901 tttcatccgc agcgagcgaa gccacatcgg gcaaggcgtt gactagaaaa tgaaaattgg
     6961 tttcagccat ttttttcacg gaacagggca gcaactgcag gcttcaggta tggctcaaga
     7021 aactccatca gcgctgcctc gctaaagtca agctcgtagc cagcttcttt ctggacgact
     7081 gtgaatcccg accggactgt gccagactta atctcgagtc cattcaaccc gctaccgagc
     7141 ctcgccgaca actgggcagt tatctgttcg cgaagcgcct cgggaaccac gagcgaaaag
     7201 gagcctccgc caccggttcg gagcagctgt gtaattacct cactgataaa ggtgacatca
     7261 gatgctgctg tagccaccgg ctgcgcaaca gctctggcag taatcaacct gccgatgtca
     7321 cttttgagct ggctgagtcc gcgcgacccg agcagccgaa gatcagattc cgcggtcttg
     7381 agcagcttgt ccgcttcggc ctttgcccgc cgcaatattt cttctgcctc actgcgggcc
     7441 ttgccggtaa tctcttcagc gcgcgcctcg gcggccgaca ctatctggcg cgcacggtct
     7501 tcggccttgc taataccctc ctggtagata cggtcaacca tacgctcaag ggtattctgc
     7561 atggctcgcc tttctgtatc gcagcatcat ggacaagtaa tttttgtccg gctgtaaggg
     7621 aagacaccag gtgcggctgc cctctaaagg ctgagcctgt aacttgcagg tgttttctaa
     7681 atcagggttt ttccgggcgc ttcaggtatt tcattagcac agcctcataa tcggcagtcg
     7741 ccacccagta gtcgagttca gcagaaacca tgcggagcgc aacttctgct gcggcctgtt
     7801 cgcgcagatt cacgatcagt agcgttgagt caccgagatc aaacctcgat ttttctgcct
     7861 tggccagatt ttcagcaact accagctctt gccggataat atccagacgc tgcagagagt
     7921 tttcgagcgc gatcagcgcg tcattggcct caatgacaat cttgtctttt tggtagctat
     7981 gcttctggcg gagaatttca attttctgct cggtgccgcc aattttgccg tcttgcttac
     8041 gcgtctgcag tggcacgttg aagacaacgc caaattcagc ctcccaggga tcgcggcgca
     8101 gtgctccctg cgcttgctgg tcctggacgg aacgcgaacc tgcaacaatc agatcaatct
     8161 gcgacccgcg ctggttctca tgaaaacgca gtgcgttctg ctcaagttcg atttcgcttt
     8221 ctatgcgctt gagctcaggc cggtgcttca gtgcaaaggc cttgtctgct tcaaaagttt
     8281 cttttgccag aggcttttcc acgggaaact cgggcggcaa ctggccggtc actggtacaa
     8341 tcatcttgcc atcgacctcg cgaaaataca gcgagaggta aagcgctgat ttctgcagca
     8401 acctgtcggc agcggcaatc tgttctttcc gggcgagtat tgcgcgttcg ttctctttct
     8461 cttcgatcgc cggtatgtca cccagcttca cgcggcgttg cagctgatct ttgcgcagct
     8521 ccgcaatttt cagcagatcc tgcactatgc gctttctttt tcctgccgct acccagttcc
     8581 agtaacctgc agccgcgtca cggtagacgc caagccgctg ttcgtccagg acgttaccgg
     8641 caatgttttt gttgatctgg ctctgctgca gttcaaggcg gtcgctgtct atgctacgat
     8701 cgcgccaaag cggtatgcgg ccaccaaaac gaatctcgcc acctgaagtc gtgcgccgtg
     8761 gcgtgtaata atccggaaaa tcaccattac ttacgcggta cccggcgaaa aaagaagtcc
     8821 cccagatagg tgtgggcttc acaatgttca tttcagcgct gttagtatta tagtagcctg
     8881 ttgtgttgtt gaccgaacct ctgagtgcca ggtcgaacgc cccctgcgct gccagtatgt
     8941 caaattctgc cttgcggatt tcacgcgttg caagcaaaat taccggaaag gttttttcaa
     9001 tattgccgag cacatcttca agcacaaccg tcttagcctg ttcagcgggt cttgcctgcg
     9061 caaggattcc cgctgcagca aacaggcagg cgataataaa gagcaagcgc ccatgcatca
     9121 tttctggctc cgcatatcca cgggaatctc tggtggaaaa tcattgaact tacgccataa
     9181 ctcgtagccg atagaaaccc tattcagaaa gacccagcca cgcgcccgga taccctggcg
     9241 cagcatcaag gtatcgggcc acggttcgtc tggtttttca tcaggtatga gcagcacgcg
     9301 aaattttcca tgcccgtcgt cggcattgtc gacgaacttc acgcgcgcgg ggaaagtacc
     9361 aaatgcgcct tcgggccaac cggaaatctg caatgccggc caaccctgga acatgatgcg
     9421 cgcctcacgg ccatcgcgta caagggtgat atcgttacct tctagccaaa gttcgacggc
     9481 acgccggccc gattcgggta tgagaatgca gaggccctta ccttcccgca caaattccgc
     9541 ttcgtgcgag accagcagcc gggtaataat accgtctttg ggagccaaaa cttcctgcgt
     9601 cttttgccgt gacaggcgct gctcaacgcg cggcaggtct tcgttgctgc gcgcaagctc
     9661 agagcgagcg ctctgtacgg ttgcgcgcgc atcgttgatc gcggcatctg catcacgggc
     9721 aagacggcgg gcatcgcgct gcatcgcctt cttttcttct tcggctgcct tcaggccggc
     9781 tacagcgcgc tgctcttcag tgcgcgtacg tttcagctcg agctctgcta gctcgacatt
     9841 gcgctgcgaa gtcagcccct gttccatcaa agatttttgc cgttcgtaat tgagctttgc
     9901 tgtctccagt gcggcctctg ctgccgcaac cgtctgctgt gattgcctga cccgctcgct
     9961 cgccatcgcc acccggtttt ccgcagcact gacggcttcg ctgcgcgcga gttgcagtga
    10021 ggtaacacgc gactcaatcg attcaacccg gttgcgtgcc gcacgcatac gatcagtcaa
    10081 cgccgcgcgc tcctgctcga ggcgttcggt caactgcggg tcaaggtctg agattgttac
    10141 gagcctatcg ccttttttga ccaacgatcc ttcatgtaca tgccagtgta caatgcgccc
    10201 ggaaacgggc gcctctatag tttgctgtct gtctgtcggt gagaacgcta caaccctgcc
    10261 ttcaccgtaa gacgtctgct gccatggcag gaacaaaatt gtcaggagca caattccggc
    10321 cgcgatgaat acccagcgga aattcatgcg ctgccaatgc ggatgcagat aatccgtgaa
    10381 gctgcggctc gcctgacctt cgtaggcttc aagctctcgg tgtgtctgct ccggtccaag
    10441 ctggtgcagc ttgaccagtg aaacactgat gcggtagaaa ttctcaaagt atttgccgaa
    10501 gcgcgaaatc gtgtttagca tgtgtgagac aatgatctcg gcagcaacca actggcctag
    10561 actcatctct ttgcgcaaaa cgagtaaacc acccaggatc aggacgatgc cattgccgag
    10621 aacaaacagt gccgcgaggc taatgtgctg cctaaagagt actcggaaca gcaggctgcg
    10681 tttctcaaga taatcccggc caaggctctc tgcgtcttct tcactgggct ttttgcgccc
    10741 gatattgccc tgcaaccatg cgagcatcct atacttgctg tcagattcgg caaagctcgg
    10801 aagaaatcct tccttaaaga gggtatagcc aatcaggtag ccgccaatca gaatcaggac
    10861 cagattataa tagataaaat acgggtgata aagggagatc acggccattc caatcacgat
    10921 tccaataagt gcagtggatc cgtcggtcaa tagcgagacg aggtttttct gcagggtaaa
    10981 aacctccatg aaatagttca ttttatcgcg ctcatcagcc ttcgtaaccc gcgaactatc
    11041 agctgaggca atcagcaccc gcgcgtacag gcggcgctgg agtatttcaa taaaccagaa
    11101 ttgcagcagc cggatcactg cgctcgctac aagaaagacc aatacgagaa ttgtcagcac
    11161 cacgactggc tgtaccaggg taccgaaaga aacagagtta atgagattgc cgacagcaat
    11221 gggcacgatc aacgccaata acccctgcac tataccaaaa ccaataatcc ggatgaattc
    11281 gcgccgctct agctggagca gccggcgcaa ctcgaatatt aatgtctccg ctttggccgc
    11341 catattgtgt tcctcaatat actaaatcat gatctattct tcaccttttc ctggcctgta
    11401 aaggattatg agcgccttgc acagccagtt ccggcacgct gtcggcattt agctgaaatc
    11461 cttgagtctt ttttttgcct tctagaaaaa agtgcaccgc ttttttcacg aaattcatca
    11521 agtaaaatat ccagccgccg acattaatgg taagactggg cagtctttct gcgcaagcag
    11581 tgggacggcg agacaggagg tctcgcgcca acgaaggttt gttaaacgca aactacagtg
    11641 gcccgggcag aaaaggtttc acggaggaaa cacaatgatt atcaatcaca acatgtccgc
    11701 aatcaatacg caccgcgtat tgaaattcca gaactggaat ctggataaag acatggagaa
    11761 gttgtccagc ggtatgcgaa ttaaccgcgg tggtgacgat gcatccggtc tcgcggtatc
    11821 tgaaaagatg cgtacacagg tccacggcct gcgccgcgct gaacagaaca ccgaagacgg
    11881 tatgagcttc atccagacga ctgaaggcta cctgcaggaa actgcggagc tcgttcagcg
    11941 tatccgcgtt ctcgcagtac aggcagcgaa cggtatctat accgacgaag accgccagca
    12001 gatccaggta gaaatctctg aactcatcga cgagatcgat cgtattgcat ctcaggcaga
    12061 gttcaacaag atgaaactgc tcactggttc tttcgcacgt ctgaacccaa ccgcatcaat
    12121 gtggttccac atgggcgcca acatgcacca gcgcgagcgc gtgttcatcg aaacgatgac
    12181 atctgcagcg ctgaagctga gaaacccgac agtgttgaca ttcgtctcga tttcaacacc
    12241 gggcaaggca aactctgtta tcggtctctc ggacgaagcg ctgcgcatca tcagcaagca
    12301 gcgtgctgac ctcggcgcgt actacaaccg cctcgagcat gcagcaaaag gcctcatgaa
    12361 cgcttacgag aacatccagg cgtcagaaag ccgcatccgc gacacagata tggcagaaac
    12421 catgtcgagc tacacacgtt accagattct cacacaagct ggtaccgcga tgctcgcgca
    12481 ggcgaaccaa cgcccgcaga gcgtattgca gctgctccgc taagcggatt aaggtaccca
    12541 accgaaaggt tgggtacttt tttcatgcga cccatggccg catgaaaaaa gtacatcgcc
    12601 gtacgactct gaagcctgaa cccaaaagtt cgggcttttt ttatgcgcaa atcagcattt
    12661 ttggacgtaa ctcatatatg gaaatctacg tcggtgatga acgcctgcaa ttcgaactga
    12721 cggagaccct ccccttttcc ggaatcatgc agaaactgca ggcctgggca gagagccaga
    12781 acctgtatat tgttgactac cgagttgcgg cgaaaccagg atttgagaat aaagaagacc
    12841 tcacttctga cgagattgcc gtcatcaacc tgcaactggg tgaccagcgc gaactcattg
    12901 aatcgaacct gcgcgaactg atcgactaca ctgaccgcgc gggttttcac ctggctggcg
    12961 tgatacagtc gggtaagatc ctaagtacac aggaagagca ggatctcacc acaggtggag
    13021 tttttctcgc ggaatcgctg cagaccctgc agacatacct gcgaccagac aaccccgaat
    13081 cgctggcgcg cgcagtgaaa gagctcaata gccatcccga cctgattgaa aagatcaatg
    13141 ccctggcgct catccagaac caactgaaga tctggctacg gcagaccgaa ttttcgcgtg
    13201 tcagtcccga agaggctaca gaacgcgtgg cgcgattccg cgagcaggtt cccggcatgc
    13261 aggaagagct cgaaaaaatt gccgcccgtt ttacccaggg ccgcgagcag gaagccctgc
    13321 aaatgctcga aacgatttcg cagacactgg tcgatgcggt agttctcatg cggatcgcca
    13381 acgataagag ccagggcgca ggagaaaaac ttgtcgacct cttgggccag ctcactggag
    13441 cgattgattc acgcgatctc gtaaccgctg ccgacatcgt cgacttcgac ctgcgcgacc
    13501 tgctcaaaga gctttaacct cacggtagta aaaccgtcgc taccctggtg ttctatgcgc
    13561 cgggaatacg tcgcctcagt gatcgtcgcc gacctgcgca acttcaaaac ggtcgacatg
    13621 ctcggcaaaa aacaactgcc tcagcttctg catgctgtcg gtcatgtcag acacaaatag
    13681 atctacccgc gcgtccgctt gcctacgcaa caggtcacgc tcacccaaca gttgcttgag
    13741 ttcgcgcgcg gtctcttcag cgctgtcgat cagtttgaaa cgcgggaaca ttctcgatat
    13801 ggtcggagca atgagtggat aatgagtgca acccaggatc aggtactcgg cgccctgccc
    13861 tgccagcggg tccaggtagt cgtgcaatat gagctccgcc gctttactgc ctgcatagcc
    13921 ctcttcgatc agaggcacga gcagcgggca tgcctggcta atgagctttt gccggccgtg
    13981 gttacgctcc agcgccgagt ggtaagaatt actggcaatc gtacctttgg ttgcaatcag
    14041 gcctgcgaca gaacctgggg ctgcgcgctg cgtcagtgtc ctgactgccg ggtcgatgac
    14101 tcccagcacc gggatatcgg tcatctcacg aatcgcgtcg agcgcataag acgatgccgt
    14161 attgcaggca accacgatgg ccttgatatc gtgcgagagc agaaagcgca caatctcccg
    14221 ggaatagcgt atgattgtgg gcgcagattt gtttccataa ggaacacgcg ccgtatcgcc
    14281 gaagtagaca agattttcgc cgggcaactg ccggtggatt tcgcgcagca cggtcaggcc
    14341 acccaggcct gaatcaaaaa caccgatgct cttttcattc tgcgccatgg ggctgccgcg
    14401 acctacgcgg cgattagctg cctcagggca ccttgtcgag accaaagact gcgtgcacga
    14461 ggcgcagcgc gtctttgccg cggtctttcg cgatcacgca ggagattttg atctcgctgg
    14521 tcgagatcat ctcgatgttg atgttattgt cagccagcgc cttgaacatg cctgcggcaa
    14581 caccggtatg cgacttcatg ccgacaccga ccgccgatac gatggcgatg ttttcgtcag
    14641 cagagatcga acccgcgccc cacttcttga tcagttgctc gagtgtctgg cgcgcctcgc
    14701 ctactgccgc aacgggcaga gtgaaggcaa tcgtgttcgt tttgtctttg ccggtcgact
    14761 ggacgatgac gtcgatgttg atctttttgt cagccagtac ctggaagatt tctgcagcga
    14821 tgcctggctt atcgggaata tcggcgactg tcatgcgcgc gtcgtctgat ttgagggtga
    14881 ctccggtgac tgtaattttt tccataatct cgttctcgct gactaccttt gttccttcgt
    14941 tataattaaa actcgatttg acatggatta tcacgccgta tttttttgca aattcgaccg
    15001 accggctgtg cagcacaccg gcgccgagcg atgccagctc gagcatctcg tcgtagctga
    15061 tctgtttcag cttgcgtgca ccttcgacct tgttcggatc agtcgtgtag accccgtcca
    15121 catcggtata aatttcgcac tctttggcct tgagcgccgc agccagcgcc acggccgtcg
    15181 tatcagagcc accgcgaccg agcgtcgtga tgttctggtc ctggtcgata ccctggaagc
    15241 ctgcaacgat acagacatgc ccctggtcga ggtgcgaact gagcctcttg gtatcgatac
    15301 tctctatgcg tgcattggaa aagttaccgt cggtaacaat attgacctgg ccgccggtaa
    15361 agctccgagc gggcacctga acttcgtgca gggccatcgc caggagcgcg atcgaaatct
    15421 gctctcctgt agaaagcagc atatccattt cgcgcttcgg cgggttggtc gatattttac
    15481 ctgcaagatc gatcagttca tcagtggtat cgcccatcgc cgagaccact acgacgagct
    15541 ggtagccctt ttcgtagtag ctcttgatgc ggttcgcgac gcggcgaata cgctccgggt
    15601 caccgaccga agtgccaccg aatttttgta cgagtatctt gctcatggct ttggatgaat
    15661 ttgcgccagt ctgtcagggc gagacagggt gtcagccgta gatgatcttc accttttcca
    15721 tggctgatcc tgccctctgg cgggcatcgt cggtattctt gccggtagcc aagacaaccc
    15781 cgaggcgccg ctcgccgtct atttcgggct tcgagaacag gcggagcgac acctgaggat
    15841 cggacaacgc tgcctcaagg ccagtatagc tgaggttctg cgacttaccc tcgccgagaa
    15901 ccacatacga cgccgaggga gccaggatct ggatttcggg cagcggcagg cccagcaggg
    15961 cgcgcgcgtg cagcgcaaat tcagaatagt tctgcgaaat catagtgacc atgccggtat
    16021 cgtgcggccg gggggaaact tcgctaaacc aaacggtatc ccctttaata aacagctcta
    16081 cgccaaagat gccaaagccc cctaaggcgc cagtcactgc ctctgcgatg cgctttgatt
    16141 ctgaaagagc cgctgcactc atcggatgtg gctgccacga ctcgtggtag tcgccgtgca
    16201 cctggcggtg gccaatcggc tcgcagaagg aagtccctac ggtgtggcgc actgtcaaaa
    16261 gagttatctc ataatcaaaa tcgatgaaac cctcggcgat catgcggccc gacttgcccc
    16321 ggccgccttc gagcgcgtac tggaaagcgg gctcgatatc ggccggtgtt tttaccacgc
    16381 tctggccttt gccagacgaa ctcatgatcg gcttcaggac aaagggaaat ccgagcttat
    16441 ctgcggccgt cttaagctct gcaagtgatt ccacgaagaa atagggcgaa gttctgatct
    16501 tcagctcttc agccgcaaga cggcgaatgc cttcgcggtt catggtgagc tgcgcggcgc
    16561 gagccgtcgg cacaacacga actcctgcag attcaaactc cgccagcact tcggtggcga
    16621 ttgcctcgat ttcgggcaca atgaaatgcg gatttacttc tttgatcaac gcgcgcaatt
    16681 tgtcgccgtc gagcattggt atcgtatagg cacggtgcgc aacctgctgc gctggtgcac
    16741 cttcgtaccg gtcgacagcg atgacctcgc agcccaggcg catcagctcg atcacgacct
    16801 cgcgccccag ctctcccgag ccgagcagca tcacgcgcgt ggcgccctgt ttttgcggag
    16861 ttcctatgct gagaggcata cgggcgagtt cggtagccga gtggcggtgc aaatcaaatt
    16921 gaaataaact gcgcctggat ttgctcgtgc tatgcggttg acgtggcaat gggggcagtc
    16981 acaagtaact atggtcaaaa cctctgccta ccgttggata attctcatcc tgttcatgct
    17041 gccagccgcg atgagccaaa tgctttggct caattttgct ccgctcctga ccgccatgca
    17101 acagaagtac aaagttgatg agctcacttc taacctgctg atcatggtct tcccgctcgt
    17161 ctatgtgctg gtctcgatac cggcaggctt tctcactgac aggcgcggct accggtatgc
    17221 tattacgctc ggcagtg
//
LOCUS       DAOQJF010000018        17204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1572_length_17204_cov_4.852061, whole genome shotgun sequence.
ACCESSION   DAOQJF010000018 DAOQJF010000000
VERSION     DAOQJF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1572_length_17204_cov_4.852061"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..620)
                     /locus_tag="PLF85_01875"
     CDS             complement(141..620)
                     /locus_tag="PLF85_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18433.1"
                     /translation="MHAAESTVSRPHRTGLHRFLARFELWTAALAVVLLVSCSLTIIL
                     VRMLSQTPGAVSLVDSLSQYPSHLMLVSALLGGSLALSRGEALKIEVLNSLLGEANKK
                     RVARLVSVIGLLFYLGFITLALRYLTIDYRPVVAFIYLPLFCLIGLKLLLAGFGAQK"
     gene            714..2027
                     /locus_tag="PLF85_01880"
     CDS             714..2027
                     /locus_tag="PLF85_01880"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HNE18434.1"
                     /translation="MADETQTADSARDFYGIDQSELPAKSSGREHNAFDPAVRLSLKE
                     IREIDEKYVLQTYARMPVAFQYGAGDHLYDTEGKEYIDFLSGIAVTALGHAHADLIAA
                     LTQQAEMVWHTSNLFYNQQQAQLARALVEINFPGKVFFCNSGTEANEAAFKFMRAYGQ
                     TKSPARQKVVAVREGFHGRTFGAMSLTGQDKIQKGFGEIVGHIEFVNADDLAGLSAAI
                     DSTTCGVIFEPVQGESGVIPLSADFIKLARARCNEEGALLCFDEIQIGMGRSGHYFAY
                     QHYGVLPDVVTMAKGLGGGFPIGAMLVAEKYATVFEKGMHGTTFGGNHLATAVAYEVI
                     RTIESQKILDHVQASARYLTSALNKLKAKYPDKITEVRGLGLLIGLVLRDDIEARPLI
                     EKALQLGLIVGRGGNSVLRLAPPLNVRKSTMDAAVQKLDQLIAAL"
     gene            2056..3153
                     /gene="aroB"
                     /locus_tag="PLF85_01885"
     CDS             2056..3153
                     /gene="aroB"
                     /locus_tag="PLF85_01885"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNE18435.1"
                     /translation="MAIKPIRVNLAERSYEVTYAESFSALKGIRQDNQFVLIDARVDR
                     LHGSEFRRVLKGTPFLRIPSGESSKNFALLEKTANELVRLGANRKSTLIAIGGGVVGD
                     FTGFLAAIFMRGIKFIQVPTTLLAMVDSSVGGKTAVNIRAGKNLMGVFHQPAAVYILP
                     AVLKTLPPRELGCGLAESIKTALIADEELCRFMENHDHDTRRFAGEFLARLSAECIAI
                     KARIVEEDEKEQSVRAFLNFGHTLAHALEARAGYAGILHGEAVSIGMRFAALLSRRLG
                     FLPGADETRIDDLLKQYALPGRMADFMAKTRMRTLPQARQLVQLMRADKKNDSGSIRY
                     VLLDGIGRARLPQAVAEKELLASLKEFQTLA"
     gene            3156..3581
                     /gene="aroQ"
                     /locus_tag="PLF85_01890"
     CDS             3156..3581
                     /gene="aroQ"
                     /locus_tag="PLF85_01890"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNE18436.1"
                     /translation="MDLVVINGPNLNMLGRREAGFYGTFTLADLEKRLREHVGNRAQV
                     SFFQSNSEGDIINHIHTLTENHRLIINAAALTHTSIGIRDALSAVKTPFIEVHISNVY
                     AREDFRQHSYLSAIAQGVIVGLGAEGYLLAADFFLKQKN"
     gene            3591..4763
                     /locus_tag="PLF85_01895"
     CDS             3591..4763
                     /locus_tag="PLF85_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18437.1"
                     /translation="MNQPLISKRSEGNYELSETGLSRAYLMTMQRLLVHYAQQALKTP
                     PTEAQQLGAKLPAFTAQVIKKLDDYFDQLPQFAAFIKEPNAGRLGAYIGQHFMPQLEK
                     VKEKLLETEIPKFKAEYENPYRVPSNDLLMLFTADFAKAPPLTYKPFDPEAEAEEKLK
                     AAELVLPGETPQGSGPKPQPGEVLLQRYTSLFITARPLSYTPDTGSEDGDDSGEISAP
                     AVPQQISFRNFVAVINTVARFIKAQDQQGYAKYYGSLPAVQQAAYTYYTLFQKAAQQQ
                     DVAWSDEIHKIMQRAKLDAGAANKLKDEVEAYRKVAQSIQRALAAGQQKGMPPQLAQS
                     LYPQLITIFENSGNLAEKRTALKMSLLQVTVTAAKDAITEILSGQLEELAGIYGLQ"
     gene            4829..5887
                     /locus_tag="PLF85_01900"
     CDS             4829..5887
                     /locus_tag="PLF85_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNE18438.1"
                     /translation="MNLKDRLKAYQKTGTGKATARPPSGTERHHTLLTTVYKEHPVSR
                     EELKLPDPVTRDFAQNVAVSELLFFDLETTGLGSAEQVYPFLIGAARQNESGTELNTW
                     FADTPAGEEEILAQFVQMASGRVLVSFNGKSFDLPLVLRRCEKYGISHNLTQALHIDL
                     FHTIRRIFPEKPARLSDAETRLLQFTRNDDISGAAVAQAYFEYLRFGKSELRTAILRH
                     NESDVLSLVSLLGKVSAAFDAARSGNKSWAYKIHRDKSASTSQKKQLLEEKPWHELDG
                     RDLHALGVIYRREKNLHRASRAFAAAYRRGYPQAIVDAVRCLRKLPGRTKSARALARY
                     GLAREDERIQTKLLPFGN"
     gene            5891..6724
                     /locus_tag="PLF85_01905"
     CDS             5891..6724
                     /locus_tag="PLF85_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNE18439.1"
                     /translation="MWTKIRFYTEVLSLDICAGVLGSAALAKTLLQAKMKPCWWFLLP
                     ASVWVIYTADHLFDARKTGSAAVNPRHKFHSDHFLPLTVAAATVSVGCVVGAFWCLRD
                     IVFLGGAFMGTLAVAHMALAYWGKIRIGKEVSVAVVYTCGIWFAPFLNRSVPISPEII
                     LAFLLFLLATILNLFMNSVIEFSLDAEEEQVFILKTIPPTIARRTVMMAAVAATLAGL
                     LIASALFPVGHRWEFTYLMFLCAVPGCILLFQDFFSVKARYRLPAELSFLAGIALLAQ
                     P"
     gene            complement(6766..8079)
                     /locus_tag="PLF85_01910"
     CDS             complement(6766..8079)
                     /locus_tag="PLF85_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNE18440.1"
                     /translation="MGFLENVKTSVAAAGRIAQTSVVFARHASFFTRLVSDPGSTENA
                     RELREIFEALGATYIKLGQFIASAPGIFPDAYVTEMQALLDRTSPVDFDDLDKVLSGE
                     LGKDYRKLFRTIEEVPLASASIAQVHAATLADGESVVLKIQRPGIEAKMDADLGLMSF
                     AAKLFEILLPETKRVGVTDVIHELGRSMADETDFIKEAHNIREFDDFLAKIGEEEVIV
                     PKVHAKLSTKRVLIMSRLYGSPMTDLATVRRYAKDPQAVLEKALSTWFQSLAMVGFFH
                     ADVHAGNLMILEDGRIGFLDFGIVGRFSPKVFESLWLLVQGVGERDYNMIARSLIGLD
                     ATGGGGEKVEEKKFAAELRTVFEKVGSFGREVEKAASRGDNINVNEADLNDMLLQIIG
                     VARDNGLRIPREFALLIKQFLYFDRYAQILAPGMSVYQPKFLPQG"
     gene            complement(8131..10854)
                     /locus_tag="PLF85_01915"
     CDS             complement(8131..10854)
                     /locus_tag="PLF85_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNE18441.1"
                     /translation="MQGKLNFDSGYQPTDARPAMRATLAGEAETYEVEIRLPAARPGE
                     QGITCSHSGLYARLWALMESYYRDPLAVFSSSRTMLLPHQVEAALRVVSASRPRFLIA
                     DEVGLGKTIEAGLILKELKLKHGYKKILIVVPSPLMAQWQQELKTKFAEDFTILNGEI
                     IRRGNPFAQGSQFLVSVDLAKDERYREMFLEQNFDLVVFDEAHRLRRDANTVTQAWQF
                     AHAISEQVEGLLLLSATPFRGKLEEIFFLIHLIDPDILGPLATFQTQFAEDASLLKDR
                     LSPVVIRRRKIDVGGFTKRFAKTVKIDLDRDEREFYDKMTEYVKTEFNKALSRGENLK
                     SFIMIVFQKLLDSSPYALMKALEGRRARLEGLYFRVMHEIAESEDVAEAVREYQEEEG
                     FEEGGMINPQELRQEIQYLTHLASLGRRIKSDSKLKHLQKTLKTMRGMGHEKFVIFTQ
                     FKSTLEYLAQNLTGYKVIGFHGGLNFDQKEDAIKKFFTEADILICTEAGGEGRNLQIA
                     ACLINYDLPWSPLKLEQRIGRIHRFGQTRDVHIVNFACRDTVAERVVEVLEEKIRLFE
                     NALGPSDTLLGVFESEYKFARSLMSFLGAKKTKREHNEELERSLFLAKENLRNVDRLI
                     STEHMSFNLAAFRQAHNGEEMRRSGEELRRLITDYAALRGITVKEGANDYQIHLEEAG
                     AKKTRVRRGTFDHDLATRRVDLEFFSFGHEFIDECAAAIIAEAEKAPVVRVLSHRDGM
                     LFFFSMTLELDKKYRRMYRIFVPRESSAGDLRPEEALRQLSQPVTGVTVDEEYLKLVT
                     MRALEKLSAEMENDVRAILVKIRPSESYWHRRIVDSAAARKSQLEERLEVQRGKLHWY
                     GDKYAGSVSKLAGEKRRSEQKAYERLQKSDSRLKPRIVIDVKKICVFSSAN"
     gene            11121..12272
                     /locus_tag="PLF85_01920"
     CDS             11121..12272
                     /locus_tag="PLF85_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18442.1"
                     /translation="MKFNKIEVNQDAKRINGEIENYLNFVVRNIDKVLGATGLGGRKA
                     DYDDEHYEFIGGANDELRKKHYDKSLRLLWKAGQHASWSTFHDCSKDEEMLLKMADQG
                     LNEAEKKQMERMQSAEFKELLNREYTPAQKQAIVNILALIGHGEAYAWLVSNEVLRDV
                     KSTGAKAALTMQVLEEAKHFVVLRELIHAFDMPIPRLPVYEYIMLETTLKSKGLEKFF
                     GMNVLVEGIALSIFGALSKYPGLEVLEMFHLDESRHCALPTNYLRTAPLTWWESNNPV
                     AMFKRFFMVLPAIPLAFKMEPDFAELGIDVFDFGGSLVRKVINLAYRVGFNLPLPQEN
                     MKDLINFLFNSYCSATREGHKYQQFLEREATTGERELAVEKEVFQLQSA"
     gene            complement(12366..13133)
                     /locus_tag="PLF85_01925"
     CDS             complement(12366..13133)
                     /locus_tag="PLF85_01925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE18443.1"
                     /translation="MALVTGSSRGLGLAIAHELGRAGYAVILNGRDLRRLEEVSAQFS
                     TPVALLLADVAEPGFGRKLLGLARQLRINHIDVVVHNAGINHMGRIGEIKPANAERTF
                     HTNTFSLIHVAQGTEAWLASAREPRFVLVSSLMQHFAMPGRSVYAASKAAAEMFARAW
                     AQELKAQKSRIRVQIFRPAGIETNFHHNTPTDGAGPRSNVSRMPPEKVARYVMKLIRS
                     KRSELAPGFGNRLVAFVARHLPALADYIAFRRYSRHF"
     gene            complement(13166..13957)
                     /gene="panB"
                     /locus_tag="PLF85_01930"
     CDS             complement(13166..13957)
                     /gene="panB"
                     /locus_tag="PLF85_01930"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004757863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNE18444.1"
                     /translation="MNIHDFGKKKAAGEKISVVTCYDNVFARLVARTSVDAILVGDSL
                     GNVIQGNPNTLAVTVDDMVYHARAVRRGAPQAFIVVDLPFMSYQTSVEDALRNAGRIV
                     KETGADAVKLEGGEAVVPQVRALVTSGVPVMGHLGLTPQSLLALGGYKVQGKTNETAK
                     KMIADAKALEQAGAFAIVLEMVPAELAKEISAALTIPTIGIGAGAGADGQVLVLTDLL
                     GLDPDFTPRFVRKYAELSGTVLTALEQYAADVRDGKFPAEKETFH"
     gene            complement(14027..14395)
                     /gene="folK"
                     /locus_tag="PLF85_01935"
                     /pseudo
     CDS             complement(14027..14395)
                     /gene="folK"
                     /locus_tag="PLF85_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
     gene            complement(14509..15180)
                     /locus_tag="PLF85_01940"
     CDS             complement(14509..15180)
                     /locus_tag="PLF85_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18445.1"
                     /translation="MKVCARLALILLFPALLHAQLRLDYYYDQSGMFTKFSDWIPKRL
                     HQWSPQFVEDFFLLYSLKQLSQQEEIRRNIFFLKIALEKKFRHPREALCLIHNPQEYH
                     KYRLLMHMHINLQIMRSYLRLASLFDKRHLYFYNLDFAPELKNSFAVAEGYYRESMPY
                     WKKARELALEASSYNFEIDMPGLESIRSDILAGDIDYTVFTGNHLLRLKQKQQEVDNW
                     LAQKK"
     gene            complement(15256..>17204)
                     /locus_tag="PLF85_01945"
     CDS             complement(15256..>17204)
                     /locus_tag="PLF85_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNE18446.1"
                     /translation="EDSINKIWRVTRKRPMIQKISGFVMVMVFGPAVLAVGISFAQSI
                     LSQFASPNFKQVRIIQDSVQILGDKHVFLVQSDKGKPYKEKNILKGIDYEASNERIVF
                     DAEQNTIVDGANTQVYNSAPRADKAMLKRAVFADYARTGTREFLITDSGILLTSRDGG
                     NTYTVRRFYRAEDDRVREVAFRRIQFLTENQGLIIGSAGLILRTADGGITWEPAYQQG
                     VEGNLRQVARLRPGTWLVLGDDSTALATQDGGLTFRKYEPVTKALRSRNESLTGLAMG
                     DRLGYIVGEAGLLLMTRDGGESWKLTPMVESLFFQDVAAAPDGTAIAVGVDGLIRYTQ
                     ILPDGSVQWQNLKGKVDVDLQSVRYYAKEGSFIIVGDHYQMMQQQHDSTNLAVTKEFK
                     VIQKAPFWRRLISALGNLLIPFLVIFLLFFLLYKIIPYTVVQTKAAATGATFTSISWV
                     IFILLYKFYVTNFSKGTAALYGTLALVPLTLLLLYVSALIVLFGAEIAFFTQYPQLFR
                     MSKKAELDERHKRQLWYGLSILHKLAASFNKGKNDCTTDNLIKYTSGDQEEFKFIMDR
                     LVDRGYVTESEDHKWLLAMNPDLIEISSLVEDLDPSDYSIPEFNAKDPYMKAVKSYFD
                     QLEASRGKIFRKVTLSRLMQGEQA"
BASE COUNT         3828 a         5026 c         4723 g         3627 t
ORIGIN      
        1 ggcagagaaa cgcggacggt aggcggcaac ctgtgcgtca ctgcggctgc aggcagggaa
       61 aatgccaccc accatagcca acgtcaatat acaacgcacc aagctcaccc ggtgctcttg
      121 caccctgcta tagcggagca tcatttttgc gcgccgaacc cggccagtaa cagcttgagc
      181 ccgatgaggc agaaaagtgg caggtagata aaggccacaa ccggccggta gtcaatcgtg
      241 aggtagcgca atgccagcgt aataaacccc aggtagaaca gcagcccgat taccgaaacc
      301 aagcgtgcca cacgtttctt attagcctca cccagcaggc tgttcaggac ttcaatcttg
      361 agcgcttcgc cgcgcgacag ggccaatgaa ccgccgagca aagctgacac gagcatcagg
      421 tgcgacggat actgcgacag gctatcgacc agtgagaccg ccccgggggt ttgtgaaagc
      481 atacgcacca ggataatcgt gagcgaacaa ctcacgagca agacgacggc gagagccgcc
      541 gtccagagct cgaaccgcgc aagaaaccgg tgtaagccgg tacgatgggg acgggatacc
      601 gtgctttctg ctgcgtgcat agcgtcaaag tggcgctcga aaaggcaccg tcaaggagag
      661 cacggtcgac gaataaacaa tttgcggcct gtttcggaag ggtaaaaagg cgcatggcag
      721 acgaaacgca gaccgcagat tcggcgagag atttttacgg catcgaccag agcgaactgc
      781 cggcaaaatc ttcggggcgc gagcacaatg cctttgaccc ggcggtgcgg ttgtccctga
      841 aggaaatccg cgagattgat gagaagtatg tgctgcagac ctatgcacgc atgcccgtcg
      901 cattccagta cggcgcgggc gaccatctct acgacaccga aggcaaagaa tacatcgact
      961 tcctcagcgg tattgcagtc accgcgctag gccatgcaca cgctgacctg atcgctgccc
     1021 tgacgcagca ggcagagatg gtctggcata cctcaaacct cttctacaac cagcagcagg
     1081 cacagctcgc gcgcgcactg gtcgaaatca attttcccgg caaggtcttt ttctgcaact
     1141 cgggtaccga ggcaaacgag gcggcgttca aattcatgcg cgcttacggg cagaccaaat
     1201 caccggcacg ccagaaggtt gtcgcggtgc gcgagggctt tcacggccgc acgttcggcg
     1261 ccatgtcgct gaccggccag gacaaaatac aaaagggctt cggtgaaatc gtcgggcata
     1321 tagaattcgt gaatgccgac gacctggcgg gattatctgc ggcgatcgac agcaccacct
     1381 gcggtgttat ttttgaaccc gtgcagggcg aatcgggcgt gattcccctt tcggcggact
     1441 ttatcaagct ggcacgcgcg cgctgcaatg aagaaggtgc cctgctctgt ttcgacgaga
     1501 tacaaatcgg catgggccgc agcggccact acttcgctta ccagcactac ggtgtgctgc
     1561 ccgatgtggt caccatggca aagggcctcg gcgggggatt cccgatcggc gcaatgctcg
     1621 tagcagaaaa gtatgccacg gtctttgaga agggaatgca cggcaccaca ttcggcggca
     1681 accacctggc gactgcagtc gcctacgaag tcatcaggac gattgaatcg caaaagatac
     1741 tcgaccatgt gcaggcctcg gcgcgctacc tgacctctgc cctgaacaag cttaaggcaa
     1801 agtaccctga caaaattacc gaggtacgtg gccttggcct gctgatcggt cttgtcctgc
     1861 gcgatgacat cgaagcacgg ccgctgattg agaaggctct tcagctcggt ctcattgtcg
     1921 ggcgcggtgg caacagcgta ctcaggttag ctcccccgct gaacgtgcgc aagtcgacga
     1981 tggacgcagc cgtgcagaag ctggaccagc tgatcgccgc gctctgagca cggcagactt
     2041 ttactggccg cgactatggc gatcaaaccg atacgggtta accttgctga acgcagctac
     2101 gaggttactt atgccgagtc ttttagcgcg ctgaagggca ttcgccagga taaccagttc
     2161 gtgctcatcg acgcacgcgt cgatcgcctg cacggcagtg agttccgccg cgttctcaaa
     2221 ggcacacctt ttctgcgtat tccttcgggt gaaagctcga aaaattttgc cctgctcgaa
     2281 aaaaccgcga acgaactggt ccgtctcggc gccaaccgca agagcacact catcgccatt
     2341 ggcggcggtg ttgtcggcga cttcaccggt ttcctggctg cgatcttcat gcgcggtata
     2401 aagttcatcc aggtgccgac cacgctgctg gcaatggtcg attcgtcggt cggcggcaaa
     2461 actgccgtca acatccgcgc gggcaagaac ctgatgggcg tctttcacca gcctgcggcc
     2521 gtctatattc tacccgcagt tctcaagacg ctgccaccgc gcgaactggg ctgcggccta
     2581 gccgaaagca tcaagactgc gctcatcgcc gatgaagaac tctgccgctt catggagaac
     2641 cacgaccacg acacccggcg ctttgccggt gaatttctcg cgcgcctctc cgcagagtgt
     2701 atcgccatca aggcacgtat tgtggaagag gacgaaaaag aacaatcggt gcgtgcattc
     2761 ctgaattttg gccatacgct cgcgcacgct ctcgaagcgc gcgcgggtta tgcgggcatt
     2821 ctgcacggcg aggccgtatc aattggcatg cgctttgccg cgcttctctc gcgccggctg
     2881 ggttttttac caggcgcaga cgagacgcgc atcgatgacc tgctaaagca atacgccctg
     2941 cccggcagaa tggctgattt tatggcaaag acgcgcatgc gcacgctgcc gcaggccagg
     3001 cagctggtgc agctgatgcg ggcagacaag aagaacgaca gcggcagcat caggtatgtg
     3061 ctgctcgacg gcatcgggcg cgcccgcctg ccgcaagcag tcgctgaaaa agaactgctt
     3121 gcgagcctga aagaatttca gacgttagcg taataatgga ccttgtcgta atcaacggac
     3181 cgaacctcaa tatgctgggg cgccgtgagg cggggtttta tggaacgttt acgcttgccg
     3241 acctggaaaa gcgcctgcgt gagcacgtgg gcaaccgcgc gcaggtcagt tttttccagt
     3301 ctaactccga aggcgacatc atcaaccata tacataccct tacagagaat caccggctca
     3361 ttatcaacgc agccgccctg acgcatacct ccatcggcat ccgcgatgca ctttcggcgg
     3421 tcaaaactcc tttcattgaa gtacatatca gcaacgtcta tgcgcgcgaa gactttcgcc
     3481 agcattcgta cctgagcgcc atcgcgcagg gtgtcattgt cgggctcggg gcagaaggtt
     3541 atttgctcgc ggcggatttt ttcctgaaac agaagaacta atacctgccg atgaaccaac
     3601 cgctgatcag taaacgctcc gaaggcaatt atgaactttc cgaaacaggt ctctcgcgcg
     3661 cttacctgat gaccatgcag cggttactcg tgcactacgc gcagcaggcg ctgaaaactc
     3721 cccccaccga ggcgcagcaa ctcggggcaa aattgccggc tttcaccgcg caggtgatca
     3781 agaaacttga tgactacttt gaccagctgc cacagtttgc cgcgttcatc aaagaaccca
     3841 acgcgggcag gctcggcgcc tatatcgggc agcacttcat gccgcagctc gagaaggtga
     3901 aagagaagct gctcgagacc gaaatcccca aattcaaggc agaatacgaa aacccgtacc
     3961 gcgtgccatc gaacgatttg ctgatgctgt tcacggcaga cttcgccaag gcgccaccac
     4021 tcacctacaa acccttcgac cccgaggccg aagcagaaga aaaactcaaa gcggcagaac
     4081 ttgtgctccc tggagaaacc ccgcagggat caggacctaa gccgcaaccg ggtgaggttt
     4141 tgctgcagcg ttatacctcc ctgtttatca ccgcgcgccc attgagttat acgccggata
     4201 ccggcagcga ggatggcgat gacagcggag aaatttctgc gcctgcagtg ccccaacaga
     4261 tcagcttccg caatttcgtc gcggtgatca atactgttgc acgcttcatc aaggcgcagg
     4321 accagcaggg gtacgcaaaa tactacggct cgctgccggc ggttcaacag gccgcttata
     4381 cctactacac gctgttccag aaagccgcgc aacaacagga cgtcgcctgg tctgacgaaa
     4441 tacacaagat catgcagcgg gccaaactcg acgccggcgc cgccaacaag ctcaaggacg
     4501 aggtcgaggc ataccgcaaa gttgcgcagt ctatacagcg cgcgctcgcc gccgggcagc
     4561 aaaaaggtat gccgccgcaa ttggcacagt cgctttatcc gcagctcatc acgatctttg
     4621 agaacagcgg caacctggcg gaaaagcgta ctgcactcaa gatgtcacta ctgcaggtaa
     4681 cagtcacagc ggccaaagat gctatcaccg aaattctctc aggccagctc gaagaactcg
     4741 caggcattta cgggttgcag tagactggcg cggcacctgg tccgccgggc aggcgttctg
     4801 cagtgatcgt ggtgggagct cgtgcgttat gaatctgaaa gacaggctca aggcttacca
     4861 gaagaccggc accggtaaag caaccgcgcg accgcccagc ggcacagagc ggcaccatac
     4921 cctgctgacg acagtctata aagaacatcc cgtctcgcgc gaagagctaa aactacccga
     4981 tccggttacc cgcgacttcg cgcaaaatgt tgccgtgtcc gaactgcttt tctttgacct
     5041 cgagacaacc ggactgggta gtgcagagca ggtctatccc tttctcatcg gcgcggcgcg
     5101 gcaaaacgag tcgggcacag aactgaatac ctggtttgcc gacacaccgg ccggggaaga
     5161 agaaatactg gcacagttcg tgcaaatggc gtcgggccgt gtgctcgtga gcttcaacgg
     5221 taagtcgttc gatctgccgc tcgtcctccg gcgctgcgaa aaatacggca tcagtcataa
     5281 tcttacgcag gcgttgcata tcgacctctt ccacaccatt cggcgcatct ttccggaaaa
     5341 accggcgcgc ctgagcgacg ccgaaacccg cctgctgcag tttacccgca acgatgatat
     5401 ttcaggcgcg gctgtcgcgc aggcctattt tgagtacctg cgctttggca aaagcgaact
     5461 gcgcacggct atcctgcggc acaacgagag cgatgtgctc tcgctggtaa gccttctggg
     5521 taaagtatca gctgcttttg atgccgcgcg cagcggtaat aaaagctggg cctacaagat
     5581 ccaccgcgac aagagcgcga gtacgagcca gaagaaacag ctgctcgaag agaaaccgtg
     5641 gcatgaactc gacgggcgcg acctgcacgc actgggggtt atctaccgcc gcgagaagaa
     5701 tctgcaccgt gccagccgcg cgtttgccgc agcttaccgg cgcggctacc cgcaggcaat
     5761 tgtcgatgca gtgcgctgcc tgcgcaagct gcccgggcgt acaaagtctg cgcgcgccct
     5821 cgcgcggtac ggccttgcac gcgaggatga acgtatccag acgaagctct tgccctttgg
     5881 aaattagagc atgtggacga aaatacggtt ttacacagaa gtactcagcc tcgacatctg
     5941 cgcgggcgtg ctgggttcgg ccgcgctggc aaaaaccctg ctgcaggcga agatgaaacc
     6001 ctgctggtgg ttcctgctgc cggccagcgt ttgggtaatc tataccgccg atcatctctt
     6061 tgatgcacgc aaaaccggca gcgctgcagt aaatccccgc cataaattcc acagcgatca
     6121 tttcctgccg ctcactgttg cggcagcaac cgtgagcgtg ggctgcgtgg tgggagcatt
     6181 ctggtgtctg cgtgacattg tcttcctggg cggagcattc atgggcactc tcgcggttgc
     6241 acacatggca cttgcttatt ggggaaaaat acggattggg aaagaggttt cggttgctgt
     6301 tgtctacacc tgcggtatct ggttcgcacc gtttctcaac cggagcgttc ccatttcgcc
     6361 ggaaatcatt ctggcatttc tgctcttcct gctcgcaacc atacttaacc tgttcatgaa
     6421 ttcggtcatc gaattttcgc tggatgccga agaggaacaa gtcttcattc tcaagaccat
     6481 acccccaacc attgctcgcc gaaccgtaat gatggctgct gttgcggcaa cactggcagg
     6541 cctactaatt gcctccgcat tgttcccggt cgggcacagg tgggagttca cttacctcat
     6601 gtttctgtgc gccgtaccgg gttgcatcct gctgtttcag gatttcttca gcgtgaaggc
     6661 gcgctatcgt cttccggcgg aattgtcatt cctggcaggt atcgcccttc tggcgcagcc
     6721 ttgactttcg ggccgctggt gccgggcctg cagaaagccg taatgctacc cttgcggcaa
     6781 aaactttggc tggtagactg acatacccgg cgccagaatc tgcgcgtacc ggtcaaaata
     6841 caggaactgt ttgatcaaca gggcgaattc ccgcggtata cgcaagccgt tgtctcgtgc
     6901 cacgccgatg atctgcagca acatgtcatt gaggtcggct tcattgacat tgatgttatc
     6961 gccccggctg gctgctttct cgacttcgcg accaaagctg ccgactttct cgaagacagt
     7021 acgcagctcc gcggcaaatt tcttctcttc gaccttctct cccccgccgc cggtcgcatc
     7081 gagcccgatc agcgagcgcg caatcatgtt gtagtcgcgc tcaccgacgc cctgcacgag
     7141 cagccagagc gattcaaaaa ctttcgggct gaaccgcccg acgataccaa agtcgagaaa
     7201 cccgatgcgg ccgtcttcca gtatcatcag gttaccggca tgcacatcgg catgaaagaa
     7261 gcccaccatc gcgagggact ggaaccaggt cgaaagcgct ttctcgagca ccgcctgagg
     7321 atctttcgcg taacggcgca cagtcgccag gtcggtcatc ggcgagccgt acagccgtga
     7381 catgatcaga acccgtttgg tactgagctt tgcatgcact ttcggtacta tgacttcctc
     7441 ttcgccgatc ttcgccagga agtcgtcaaa ctccctgata ttgtgcgctt ctttgataaa
     7501 gtcggtctca tcggccatcg agcggcccag ttcgtggatc acatcggtca ctccgacgcg
     7561 cttggtttca ggcagcagga tctcgaaaag tttggccgca aaggacatga gacccagatc
     7621 ggcgtccatc ttcgcctcga tacccggccg ctgtatcttg agaacgacgc tctcgccgtc
     7681 ggcgagcgtg gccgcatgca cctgcgcaat cgacgctgaa gccagaggta cctcttcgat
     7741 ggtgcggaag agcttgcggt agtctttacc gagttcgccg ctcaggactt tgtcgagatc
     7801 atcaaagtcg accgggctcg tgcgatcgag cagtgcctgc atctcggtca cataagcgtc
     7861 gggaaagatg ccgggcgcgg aagcaataaa ctgccccagc ttaatatagg tggcgccgag
     7921 cgcctcaaaa atttcgcgca attcgcgggc gttctcggta gaacccggat ctgaaaccaa
     7981 gcgcgtgaaa aacgaagcat gccgcgcaaa gacgaccgag gtctgcgcga tgcgccccgc
     8041 ggcagcgaca gaagtcttga cgttttccag aaagcccata gaccccaaag gggcatctac
     8101 tggcggcgaa aagcgcgatt tcggccggcg tcagttagcg cttgaaaaga cgcagatctt
     8161 ttttacatcg ataacgatac gcggtttgag ccgcgaatcg ctcttctgca gcctctcgta
     8221 tgccttctgc tcgctgcgcc gcttctcgcc cgccagcttc gaaacgctgc cggcatattt
     8281 atcgccatac cagtgcagtt tgccgcgctg tacctcgagg cgttcttcga gctggctctt
     8341 gcgggctgcc gccgaatcga caatgcgccg gtgccagtaa ctttccgacg gacgaatctt
     8401 taccaggatt gcccgcacat cgttttccat ctcggccgaa agtttctcca gagcgcgcat
     8461 cgtcacgagt ttcaggtatt cttcgtccac ggtcacgccc gtcaccggct gcgacagctg
     8521 ccgcagcgct tcttcgggcc gtaaatcccc ggcggagctt tcgcgcggca caaagatgcg
     8581 gtacatgcgc cggtacttct tgtccagctc gagtgtcatc gagaagaaga acagcatgcc
     8641 atcgcggtgc gaaagcaccc ggacaaccgg cgccttctct gcttcggcga tgatcgccgc
     8701 ggcgcactcg tcgatgaatt catggccaaa cgaaaagaac tcgaggtcga cccgccgggt
     8761 ggcgaggtca tggtcaaacg taccgcggcg cacacgcgtt tttttcgcgc cggcctcttc
     8821 caggtgaatc tggtaatcgt ttgcaccctc tttgacagta ataccgcgca gtgcagcgta
     8881 gtcggtgatc aggcggcgta gttcttcgcc gctgcggcgc atctcttcgc cgttgtgcgc
     8941 ctgcctgaat gccgcgagat tgaagctcat gtgctcggtc gatatgaggc ggtcgacgtt
     9001 gcgcaggttt tctttggcca gaaacaggct gcgctcgagc tcttcgttgt gttcgcgctt
     9061 ggtcttcttg gctcccagga acgacatcag gctgcgtgca aacttgtatt cgctctcgaa
     9121 gacgccgagc agcgtatctg acgggcccag cgcattttca aacaggcgga ttttctcttc
     9181 gagcacttca acgacgcgct ccgcgacggt atcgcggcag gcgaagttga caatatgcac
     9241 gtcgcgcgtc tgcccgaaac ggtgtatacg gccgatacgc tgctcgagct tcagcgggct
     9301 ccacggcagg tcatagttga tcaggcaggc cgcaatctgc aggttgcgcc cttctccgcc
     9361 tgcctcggtg cagatcagga tatcggcttc ggtaaaaaat ttctttatcg cgtcttcttt
     9421 ctggtcaaaa ttcaatccgc catgaaaacc aatgaccttg tagcctgtca ggttctgtgc
     9481 caggtattcg agcgtggact tgaactgggt gaagatgaca aatttctcat gccccatgcc
     9541 gcgcatcgtc ttgagggtct tctgcaggtg cttgagtttg ctgtctgact tgatgcgccg
     9601 gccaaggctc gccaggtgcg tgaggtactg tatctcctgc cgcagctcct gcgggttgat
     9661 catgccgcct tcttcgaaac cctcttcttc ctggtattcg cgcaccgctt ctgccacgtc
     9721 ttcgctctcg gcgatttcgt gcatcacgcg aaaatacagt ccttctagcc gggcgcgccg
     9781 cccctcgagg gccttcatca gcgcatacgg cgaagaatcg agcagcttct ggaacacaat
     9841 catgatgaat gatttcaaat tttcgccgcg cgacagggcc ttgttgaact cggttttgac
     9901 gtattcggtc atcttgtcat agaactcacg ctcgtcgcgg tcaagatcga tcttcaccgt
     9961 cttggcaaaa cgtttggtga aaccacccac gtcgatcttg cgtcggcgaa tgacaacagg
    10021 agaaagccgg tctttcaaaa gcgatgcatc ttcggcaaac tgcgtctgga atgtcgcgag
    10081 tggccccagg atatcggggt caatcaggtg tattaagaag aaaatttctt caagcttgcc
    10141 acgaaacggt gtcgccgaca acagcagcag gccctcgacc tgctcgctga tcgcgtgggc
    10201 aaattgccag gcctgcgtca cggtgttggc atcgcggcgc agccggtgcg cttcgtcgaa
    10261 caccacaagg tcaaaattct gttcgaggaa catttcgcgg taacgctcgt cttttgccag
    10321 gtcgaccgag accaaaaact ggctaccctg cgcaaacggg ttaccgcggc ggataatctc
    10381 gccgttgaga atcgtaaaat cttcagcgaa cttggtcttg agctcctgct gccactgcgc
    10441 catgagcggc gacggtacca caatcagtat cttcttatat ccgtgcttga gcttgagctc
    10501 cttcaatatc aggccggcct cgatagtttt accgaggccc acctcatcgg cgatcaaaaa
    10561 gcgtggccgc gaggccgaga ccacgcgcag cgccgcctcg acctggtgcg gcagcagcat
    10621 cgtgcgcgaa gatgaaaata ccgcaagcgg atcgcggtaa taactttcca tcagggccca
    10681 caggcgggca taaagtccac tgtgactgca ggtaatgccc tgttcgcccg ggcgtgccgc
    10741 aggcaggcgg atttctactt catatgtctc tgcctcgcca gcgagcgtgg cccgcattgc
    10801 cggacgggca tcggtcggct ggtagccgct gtcaaagttt agcttgccct gcattgtgaa
    10861 gagacataat acaggcagcc cctgctatgt aaaccgagtc gagccttctg cgcacgtttt
    10921 ttcgcatctt tccgggctgg ccagtctcct tcccaggaaa caagccccat taccacctca
    10981 atccgccaaa atgggagatc atgcaccgcg caaaaaacta tttaaaaaat tatagttttt
    11041 tagtggacaa gacgacacta aaaataaata gatcgtttaa tcttgttttt ggggctgcaa
    11101 cgcacccctg ggaggaaaca atgaagttta acaaaataga ggttaaccag gacgcgaagc
    11161 ggatcaacgg cgaaatcgag aactatctca atttcgtggt ccgcaatata gataaggtgc
    11221 ttggggcgac cggtctcggg ggccgcaagg cagactacga cgacgaacac tacgaattca
    11281 tcggtggcgc aaacgacgag ctgcgcaaga aacactacga caaaagcctg cgcctgctct
    11341 ggaaagcagg acaacacgct tcgtggtcaa ctttccatga ctgcagcaaa gatgaagaga
    11401 tgctgctcaa gatggctgac cagggcctga atgaagctga aaagaagcag atggagcgca
    11461 tgcagtctgc cgagttcaaa gaactgctca accgcgagta cacgccggcg caaaaacagg
    11521 ctatcgtcaa tattctcgcg ctgattggcc acggcgaagc gtatgcctgg ctcgtgtcga
    11581 acgaagtgct gcgcgatgtg aaaagcacgg gcgccaaagc ggcgctgacg atgcaggtac
    11641 tcgaagaagc taaacacttc gtggtgctgc gcgagctgat ccacgcgttc gacatgccga
    11701 ttccgcgcct gcccgtctac gagtacatca tgctcgagac gacgctcaag tcaaagggat
    11761 tggaaaagtt cttcggcatg aacgtgctgg ttgaaggtat tgccctgtca atcttcggcg
    11821 cgctcagcaa gtatcccggc ctcgaagtac tcgagatgtt ccacctcgac gaatcacgcc
    11881 actgcgcgct gccgaccaac tacctcagga ccgccccgct cacctggtgg gaaagcaaca
    11941 acccggtcgc catgttcaag cgcttcttca tggtgctgcc ggcgattccc ctcgcgttca
    12001 aaatggagcc tgactttgcc gagctcggca tcgacgtgtt cgactttggc ggctcgctgg
    12061 tgcgcaaggt gattaacctc gcctaccgcg tgggtttcaa cctgccgctg ccgcaggaga
    12121 acatgaagga cctcatcaac tttctgttca actcttactg cagcgccacc cgtgaaggac
    12181 acaagtacca gcagttcctc gagcgtgagg cgacgactgg cgagcgcgag ctggcagtcg
    12241 agaaagaagt ctttcagctg cagagcgcct aatgaggtca gccccgctac cgcggggctg
    12301 aatcagcgca aacggggttg gctaacgcca accccgtttg cgttaagggg gcaaagcacc
    12361 acccatcaaa agtgccgact atatcgccga aaggcgatat agtcggccag cgcgggaagg
    12421 tgccgtgcga caaaggccac cagccggttg ccgaatcccg gggcgagttc gctccgtttg
    12481 gaccgaatca gtttcatgac ataacgcgcg accttctccg gtggcatgcg cgagacattc
    12541 gatctgggcc cggcgccatc ggtaggggta ttgtgatgaa agttcgtttc aattcctgcg
    12601 ggtctgaaga tctgcacccg gatacggctc ttctgtgctt taagctcctg cgcccaggcg
    12661 cgggcaaaca tttctgccgc cgccttactc gccgcgtaga cgctgcggcc gggcatcgcg
    12721 aaatgctgca tcaggctaga gaccaggaca aaacgcggct cacgcgcaga ggcaagccag
    12781 gcttctgtgc cctgcgcgac atggatcagc gagaacgtgt tggtatgaaa agtgcgttcg
    12841 gcgttcgcag gcttaatttc gccgatacgg cccatatggt tgatgcccgc attgtgtaca
    12901 acaacgtcga tatggtttat tctcagttgc cgcgcgaggc ccagtagctt gcggccaaaa
    12961 cccggctccg ccacgtcagc cagcagcagc gcgaccggcg tactgaactg cgcgctcacc
    13021 tcttctagcc gcctgagatc gcggccgttg agtataacag cataacccgc gcggcccagc
    13081 tcatgcgcaa tcgccaaccc caacccacgg cttgagccgg taaccagcgc caccctgcgg
    13141 gaactcgaaa attcggccgc agctttcagt ggaaggtttc cttttctgcc ggaaattttc
    13201 cgtcacgtac atcggccgca tactgctcca gcgctgtaag tacagtaccg ctgagctccg
    13261 catacttgcg cacgaatcgc ggcgtaaaat cgggatcaag ccccaaaagg tcggtcagca
    13321 cgagcacctg cccgtcagcg cctgcccccg ccccgatacc gattgtcggt atggtcagcg
    13381 cagcagaaat ttctttagcg agttccgccg gcaccatctc gagtacgatg gcaaatgcgc
    13441 cagcctgctc gagcgccttg gcatcggcaa tcatcttctt cgcagtctcg tttgtcttgc
    13501 cctgcacctt gtaaccgccg agggcgagca gcgactgcgg cgtgagtccc aggtgtccca
    13561 tgaccggtac accgctcgtg accagagctc tcacctgcgg tacaaccgct tctccgcctt
    13621 caagtttaac cgcgtctgcg ccggtttctt taacaatgcg gcccgcgttg cgcagggcat
    13681 cttcgacgct cgtctggtaa gacatgaaag gcaggtcaac cacgatgaac gcctgcggcg
    13741 caccccggcg cactgcgcgc gcatggtaca ccatgtcgtc gacggtaacg gcgagtgtat
    13801 tggggtttcc ctgtataaca ttgcccagag aatcgccaac caaaatggca tcgaccgaag
    13861 ttcgcgcgac gaggcgcgcg aagacattgt cgtagcaggt cacgacgctg attttttcac
    13921 cagccgcttt cttttttccg aagtcgtgta tgttcataac tgatcttccc ctgattctgc
    13981 tgtagacgat acccgtttca tgatcgatgg cgtcgggcct gagttctgcc atgatctgcc
    14041 tgatccattc gcgcgaccgg ttaaacgggt gcggtacccg cagtgcgttg gtgcgcacaa
    14101 tacccgctga acaccaggca atatctatat caatttcgcg cggcccgttc ttgatgcgtt
    14161 ttattcgacc aatctgcgct tcaagcctca gtaattctgc cagaagctca gtgggagcta
    14221 tcgcggtttc gagcaaaaca ccctggttga aaaattttcc ctgcgcccga atgagtatcg
    14281 gccaggtctc aagaatacgc gaaacagcct tcaccgtgcc aagccgcgaa atacctttaa
    14341 tggccagttt caggttcgct tcgcggtcgc cgacattgct gcccagcgca agcacatact
    14401 caaatttttc cccgttagcc gccaaactac cccaccgtat ccggtttgag tgacacggtg
    14461 gcaatcagta ggggggctgc gcggcaggtc ccgctacccc taaggcacct actttttctg
    14521 cgccagccag ttgtcgactt cctgctgttt ctgcttcaac cgcagcaggt gattgccggt
    14581 gaacacggta tagtcgatat cgccggcgag tatatccgag cgtatagact ccaggcccgg
    14641 catgtcgatt tcgaagttat acgacgaggc ctcaagcgcc agttcacggg cttttttcca
    14701 gtatggcatc gattcgcggt agtacccttc ggcgaccgcg aacgaattct tcagctcggg
    14761 cgcgaagtca aggttgtaga agtacaggtg gcgcttgtca aaaagcgaag ccaggcgcag
    14821 gtacgagcgc atgatctgca ggttaatgtg catatgcatc agcaggcggt acttgtggta
    14881 ttcctgtggg ttgtgtatca ggcacaatgc ctcgcgcggg tggcggaatt tcttctccag
    14941 cgcaatcttc aggaagaaga tattccgtct tatctcttcc tgctgcgaca gctgcttgag
    15001 gctatagagc aggaaaaaat cttcgacgaa ctgcggcgac cactggtgta accgcttggg
    15061 aatccagtcc gagaacttcg tgaacatgcc cgactggtca taataatagt ccaggcgcag
    15121 ctgcgcatgc agaagcgcgg gaaacagcag gatgagggca agccgggcgc agaccttcac
    15181 ctaccgatta tcggcttggg cccggcaatc cggcagtact tttggcttga gtccctcccc
    15241 ttgccccgcg gggtgtcagg cctgttcgcc ctgcatcagg cgcgaaagcg tcaccttgcg
    15301 gaaaatcttg ccgcggctcg cttcaagctg gtcgaaatag ctcttcactg ccttcatata
    15361 agggtctttc gcgttgaatt cgggtatcga atagtccgac gggtcaaggt cctcgacgag
    15421 cgaactgatt tcgatcaggt cagggttcat cgcgagcagc cacttgtggt cttctgattc
    15481 ggtcacatac ccgcgatcga cgagccggtc catgatgaac ttgaactcct cctggtcgcc
    15541 tgaggtatac ttgatcaggt tatcggtcgt gcagtcattt ttgcctttgt tgaagctcgc
    15601 agcaagcttg tgcagtatac tgaggccata ccacagctgc cgcttatgcc gctcatcgag
    15661 ctctgccttc ttgctcatgc gaaagagctg cgggtactgc gtgaagaaag cgatttctgc
    15721 accaaaaaga acgatgagcg ccgatacata gagcaacagc agtgtcagtg gaaccagggc
    15781 gagcgtaccg tagagcgcag cggtcccctt ggagaaattg gtcacataaa acttatatag
    15841 caggataaag atgacccatg atatagacgt aaaggtggca ccggtagcgg cggccttggt
    15901 ttgcacaacc gtatatggaa taatcttgta gagcagaaag aacagcagga atatcacgag
    15961 gaacgggatg agcagattgc cgagagcaga gatcagtctg cgccagaatg gcgctttctg
    16021 tatgaccttg aattccttgg tgaccgcgag attcgtggag tcgtgctgct gctgcatcat
    16081 ctggtaatgg tcaccgacaa tgatgaagct cccctctttg gcataatagc gcaccgactg
    16141 cagatctaca tctaccttac cctttaggtt ttgccactgc acagagccgt cgggcagtat
    16201 ctgtgtataa cgaatcaggc catcgacgcc tacagcgatt gcggtgccgt caggtgcggc
    16261 tgccacatcc tggaaaaaaa gcgattcaac catcggcgtt agcttccagc tttcaccgcc
    16321 gtcacgcgtc atgagcaaca ggcccgcctc acctacaatg taacccaggc gatcgcccat
    16381 cgcgagacca gtcagtgatt cattgcggct gcggagtgcc ttcgttacgg gttcatactt
    16441 tctaaaggtc aggccaccat cctgggtagc aagcgcagtg ctgtcgtcac ccagcacaag
    16501 ccaggtacca ggccgcaggc gcgcaacctg gcgcaggtta ccttcaacgc cctgctggta
    16561 agccggctcc caggttattc ccccgtcggc agtgcgcagt atcaggccgg cagaaccgat
    16621 aatgagtccc tggttttctg tgagaaactg gatgcggcga aaggccactt cgcgcacacg
    16681 atcatcttct gcgcgataga aacgccgcac ggtgtacgta tttccgccat ctctgctggt
    16741 cagcaatata ccgctgtctg tgatcaggaa ctcccgcgtg cccgtccggg cgtagtctgc
    16801 aaagacagct ctcttgagca tcgccttatc agcccgggga gcgctgttat aaacctgcgt
    16861 attggcgccg tccacaattg tattctgctc ggcgtcaaag acaatacgtt cattggatgc
    16921 ctcgtagtcg atacccttca gaatgttctt ctctttgtat ggcttcccct tgtcgctttg
    16981 caccagaaac acgtgcttat cacccagaat ttgtaccgag tcctgaataa tgcgtacctg
    17041 cttaaaattt ggcgaggcaa actgcgaaag tatcgattgc gcgaagctga taccgaccgc
    17101 cagcacggcg ggtccaaaaa ccatgaccat gacaaaaccg gaaattttct ggatcatggg
    17161 acgtttccgc gttacccgcc agatcttatt gattgaatcc tcta
//
LOCUS       DAOQJF010000019        16998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1602_length_16998_cov_5.039544, whole genome shotgun sequence.
ACCESSION   DAOQJF010000019 DAOQJF010000000
VERSION     DAOQJF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_16998_cov_5.039544"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1319)
                     /locus_tag="PLF85_01950"
     CDS             complement(48..1319)
                     /locus_tag="PLF85_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNE18447.1"
                     /translation="MAASLLTTLSATPSFVQTRGMDLVRGGKKYTFMGANFWYGMNLG
                     AHDRPRLKRELDRLQKLGIKNLRILAATEGPDNEPYRIVPAMQTAPGIFDRKLLEGLD
                     YLLAEMAKRDMTAVIVLGNYWQWSGGFGQYMRWAGKGEIPYPEFDPEARGGSDYKLSS
                     WWRWFRYNYYVTRFYKNPVAVGFYHNTVRMLVTRINSVTRRAYKDDPTIMAWQLANEP
                     AGFLSGDSYDKWIAESAALIKSLDRNHLVSTGAMGEVFQFSGNDQIKNHSHKDIDYTT
                     VHIWVQNSGLYSPWKASETYAKAVEVLHKQLAQHRELAKKLNKPLVFEEFGFSRDLNR
                     FTAGTPVTLRDDFYAQAFYHVMESQKTDSPIAGVNFWAWGGEGRPARVQWQPGDNFIG
                     DPPHEAQGWYSIYDTDATTLKLIREVAGRLK"
     gene            1462..>2525
                     /locus_tag="PLF85_01955"
     CDS             1462..>2525
                     /locus_tag="PLF85_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE18448.1"
                     /translation="MQNTEKAAFTGYEKFLIAILTILQFTVILDFMVLSPLGAILMPA
                     LSINPQQFGWVVSAYAFSAGASGLLAAGFADRFDRKRMLLVFYTGFLAGTLLCGLAPD
                     YHTLLAARVVTGIFGGVISAIAFAIVTDVFAFAARGRVMGFLQMAFASSQVLGIPVGL
                     YVANKFNWHAPFLLIVGLGLPVGAVIVLKMKPIDAHLALKIDKSPFHHLLTTISKPRY
                     LRGFAATTLLATGGFMLMPFGSAFGTGNLGLSNDDLPTLYMVTGITAMIAGPIAGRLS
                     DTIGKYKIFTIGSTVGIILVLIYTQLGRSPLWLVMLITAALFASITARIVGASALMSG
                     VPAPQDRGAFMGVSSAFQQIS"
     assembly_gap    2526..2625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2626..2849
                     /locus_tag="PLF85_01960"
     CDS             <2626..2849
                     /locus_tag="PLF85_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE18449.1"
                     /translation="PQDRGAFMGVSSAFQQISGGIASAVAGAIVVKTSSGALARYDVL
                     GYVVVGAMLVTIIMMYTIHRLVMHQDHKG"
     gene            2857..3960
                     /locus_tag="PLF85_01965"
     CDS             2857..3960
                     /locus_tag="PLF85_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HNE18450.1"
                     /translation="MAEKTAAKRIGGLDALRGFALWGICVVNLPLIARSWDSYRQLPE
                     TASARGALFVNSLLFEAKFFTLFSFLYGIGIAVLQHREGTAFLLRRFLGLIVAGFLHA
                     ILLFPGDILLSYGLLGLFFLPLSRMHNRALLVTAIFCLAISGMTYTFLGVMSQANAAI
                     PPTNYTASYTAVVASNLQVYPVSLGYVMLFNWPGALAMICLGYLAERRQWLAKIRLTA
                     FSSLFALAGVTGSLLYASAASWQLKQYMPVGMLAMALTAPLLSLFYAQVILTLAEKTT
                     AWLTKALTAAGRLSLTNYVTQSLLAGIIFHGYGFGLYNKLSYDGLFLVATGIYLATIG
                     FSLLWLRYFSTGPLEWFLRSLSHWQLMPMRSAG"
     gene            complement(3957..4139)
                     /locus_tag="PLF85_01970"
     CDS             complement(3957..4139)
                     /locus_tag="PLF85_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE18451.1"
                     /translation="MQADENRKYERFLVGPHLDCKVHLKHPEALVADLSDISAGGMMV
                     LLSRNEDIDAVAEEPA"
     gene            complement(4206..4433)
                     /gene="yidD"
                     /locus_tag="PLF85_01975"
     CDS             complement(4206..4433)
                     /gene="yidD"
                     /locus_tag="PLF85_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNE18452.1"
                     /translation="MNTAIRKFFIFLIRFYQYTFSAFLGGQCRFHPTCSQYAVEAFEK
                     LPIHRAFYLTLWRILRCQPFAKGGDDPVPEK"
     gene            complement(4477..4602)
                     /locus_tag="PLF85_01980"
                     /pseudo
     CDS             complement(4477..4602)
                     /locus_tag="PLF85_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
     gene            complement(4689..5852)
                     /locus_tag="PLF85_01985"
     CDS             complement(4689..5852)
                     /locus_tag="PLF85_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNE18453.1"
                     /translation="MVHKEFDDVVYGATGFTGRLVAEYLSHASTGSAPKWAMAGRNCA
                     KLAEVRDLIGAPKEIPLIEADASSPASLKAMCARTKVVLTTVGPYQLYGTPLVSACAE
                     TGTDYVDLCGEPNWIREMIETYDDAAKKSGARIVISAGFDSIPSDLGVWFLQQEAIRR
                     FGAPAPRVKGRVRKMKGGASGGTLASMKEVMRAGMKNPSLLKIMGSPFALTPGFEGPA
                     QPSGMVPEYDRSAGVWVVPFVMAPINTKNVHRTNFLLGHPYGKDFVYDEMMYTTLGEA
                     ASALVDAAAKMNPFAGKGLKPGEGPSKEERETGFYDIVHIGEYPDGRRLQIAVKGDRD
                     PGYGSTSKMIAQAALALGETKSGGGVFTPGGVIAAPLVDRLQKYSGVTFTVES"
     gene            6041..7231
                     /locus_tag="PLF85_01990"
     CDS             6041..7231
                     /locus_tag="PLF85_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE18454.1"
                     /translation="MQHITRTIWLVSLVSLFTDIASEMLYPVMPIYLQTIGFSIVYIG
                     LLEGIAEATAGLSKGFFGRWSDATGKRVPFVRAGYLLSALAKPLIVAAAQPLWVFFCR
                     TADRLGKGVRTGARDALLSAETDPAHKGKVFGFHRGMDTLGAAIGPALALLWLWQNPG
                     RYHELFLFAFLPGLMAIALTYLLKEKASQAKVARQFRWWDFLRYYTSASVAYRHTVKG
                     LLLFTLFNSSDVFLLLVLKWRGFTDQQVIGVYIFYNLVYAVFSYPMGRLGDTIGLKKT
                     FLAGLLVFILVYTGITFARDFYALLTLFFAYGIYAAMTEGISKAWITNLCNKEDTATA
                     VGTYTAFASLATMLASFAAGLIWKFVSPEATFLVAAAGAGIAGVYLVLTPPPAPPLLE
                     EGRP"
     gene            7253..8389
                     /gene="chrA"
                     /locus_tag="PLF85_01995"
     CDS             7253..8389
                     /gene="chrA"
                     /locus_tag="PLF85_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNE18455.1"
                     /translation="MKTKLLTILALFTRLGVTAFGGPVAHIALMRHEFVARHRWLSDE
                     KFVELISLTNLIPGPNSTEMAIHLGYERAGRRGSVLAGLGFIVPAVIITLGIAWFYGE
                     YGTLPAARALLFGIKPAIIAVIVFAVLPLFKSAVNTLGKLAAAAATVVLCFLGMSEVL
                     VMLGVGVFFALAKCAATRATTRGMQGLVPFFLLQPFFTGTATALNTKLFLIFLKIGSI
                     LYGSGYVLFAFLDAELVTRGLLPRSQLMDAVAVGQITPGPVFSAVTFIGYQVNGFSGA
                     IVSTVAIFLPSFIFVIATHSLLPRLRKSTLFSAFLGGVNAASLAIMLFALVAMSRDTF
                     THWSAFMIAALSGAGLYLFKNPGSTYVVLFGAAGGIVLSYWGIV"
     gene            complement(8507..>9352)
                     /locus_tag="PLF85_02000"
     CDS             complement(8507..>9352)
                     /locus_tag="PLF85_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE18456.1"
                     /translation="KNAKQENKGGVGVFSEHGYQQLQDKSRMVDEIHRGLEQEQFVLW
                     YQPVYDARRTLYTGVEALVRWQHPRLGLLSPARFMPLVTEIGKTRELGAFVLWQACRQ
                     VADLLADGLDVGRVSVNLAAEQLYNNNLAIEIQNLLQQHGLPGHILEVEVLEELIGRD
                     FDLINEQLTRLRDMGIGISVDDFGTGYSSLSRLKLLPVQKIKIDKSFVDGLPHSVNDQ
                     SIVRSIVALGRALQLYLVAEGVETAEQSAWLEHEGVDYLQGFLYARPMEYEQLREFLK
                     KRVSG"
     assembly_gap    9353..9452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9453..10515)
                     /locus_tag="PLF85_02005"
     CDS             complement(<9453..10515)
                     /locus_tag="PLF85_02005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE18457.1"
                     /translation="MPFDMANLVSLAARHQRLMQIRYGQESHGLTAEQKLQKILHYCA
                     VELDVARAGLWRLNSAGDAITCTLQYERGKHHYTKGPELTAARYPGYFEAMRRDRIIN
                     ADDARRDPRTADLTETYLKPLGIYSLLDCPTFAGGKLAGILCIEAEAAVRTWSLLDIS
                     FAAAAADAVSAIQEQHLWEEERRYNQFLEQYDSLTGLVNRRSFQQQVFRDIAAKPDEQ
                     HLLALLGLDAFTAINDKFGQVIANSVLRVLGERASEITRRFGSASGRISGDVLGFWLS
                     PLPEQHVVDEFMSAIRKILEDAIETEYGVEVRVSGTTAVYFHTADQKAVPDPILAAEI
                     ALKNAKQENKGGVGVFSEHG"
     gene            complement(10600..10998)
                     /gene="rnpA"
                     /locus_tag="PLF85_02010"
     CDS             complement(10600..10998)
                     /gene="rnpA"
                     /locus_tag="PLF85_02010"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNE18458.1"
                     /translation="MSETPIPLKRSRYFTSLKLKNRIDHLFKNGRKSFGRYLMLRFDN
                     TTDTNPPLRVVFAVSSRLGPATVRNRIKRRLREALFLTLKEGRSNPAGFDIAIVPRKE
                     VAELDFSELCDDLRQALRRMVPAKNTGHRK"
     gene            complement(11002..11172)
                     /gene="rpmH"
                     /locus_tag="PLF85_02015"
     CDS             complement(11002..11172)
                     /gene="rpmH"
                     /locus_tag="PLF85_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNE18459.1"
                     /translation="MKRTWQPANKKRNRTHGFRTRNATAEGREVLRRRRQKGRWKLTV
                     SDERRRWDIVKR"
     gene            11220..11360
                     /locus_tag="PLF85_02020"
     CDS             11220..11360
                     /locus_tag="PLF85_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18460.1"
                     /translation="MTAEAALEFHSQTTRLPGEHFLIMSHQVIEAVVGVVKHSETRQA
                     KY"
     gene            11538..11936
                     /locus_tag="PLF85_02025"
     CDS             11538..11936
                     /locus_tag="PLF85_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18461.1"
                     /translation="MSTDYFALFPLVIFAIVIAIFVRFYRRKDQLHAAELGKKPLFSD
                     TCGGVVGWLNYKGPFIRVAVYPDFVVVSCDRQYYLPFGEIASIERRSFLFTKGYRIHH
                     RNPQYPKRIEVWLGKQESFEAALAGKVTLV"
     gene            11933..12550
                     /locus_tag="PLF85_02030"
     CDS             11933..12550
                     /locus_tag="PLF85_02030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE18462.1"
                     /translation="MIPGEPIGRKYDRIARWWHETHKDSQYGIPQIARAISYCRNKGR
                     ALDIGCGSGGRIFRLLGDHGFTITGVDASAEMIALAGMEHPELHLVHTDIREWSIDEA
                     FDLIIAWDSIFHLEEYEHEEVITKLVDFLAADGVLIYSLGDAVGSHESDWHGDKFPYG
                     SIGIDANLRLLMDLGCHIRHVELDQYPQQHAYVIAQAGEGLERVG"
     gene            complement(12556..12912)
                     /locus_tag="PLF85_02035"
     CDS             complement(12556..12912)
                     /locus_tag="PLF85_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18463.1"
                     /translation="MKKIIAKPRRLLHVCAAAIVVLGPGLGAQSKGKAPAEPTEMNLV
                     VPPDNVAMPDGKITGTLKNSRFTGTWSEAGGSTHGTFDLMTSIERKTNRPTNLRGKWK
                     NAGERDWQPTPLECSN"
     gene            13226..13798
                     /locus_tag="PLF85_02040"
     CDS             13226..13798
                     /locus_tag="PLF85_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNE18464.1"
                     /translation="MPKPTTKNTLNAKESADLLAQLKTRFEKNMARHKGLTWAAVEKK
                     LAAQPGKLASLAQMEQTGGEPDVVSHDKKTGTFVFMDCAAESPAGRRSLCYDRKALDA
                     RKENKPKGSALEAAEQMGAELLTEEDYRALQKLGAFDLKTSSWILTPEKIRKLGGALF
                     CDRRYDQVFTYHNGAESYYAARGFRCVLRV"
     gene            13799..14581
                     /locus_tag="PLF85_02045"
     CDS             13799..14581
                     /locus_tag="PLF85_02045"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNE18465.1"
                     /translation="MIAKKLSLSVTLAAAILSTALVAQKAGSEDKARKPLLGVVHTQA
                     PRGVRVEKIMQKSAAERAGLKAGDIILSVNSVAIRDPKHLFTEVQRYAAGTVVAIEYQ
                     RDGKTQLLQVPLGERLDAASLAGQTAAAFSLPQLGKKTPYALATGKVVVIDFWATWCG
                     PCEPVRRALEEFQRERRTSDIAIVGITNEDEATVRAFVRNRGARYPLLIDAKGGVALD
                     YHVAGYPTIVVIDKKGIVRFAGFASGGGLEHALQLAEKLAAE"
     gene            14592..>16998
                     /locus_tag="PLF85_02050"
     CDS             14592..>16998
                     /locus_tag="PLF85_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNE18466.1"
                     /translation="MAEQQLTKTEAAALHGIIDDVSPVTLRRAQDYARSGHVGPIARK
                     GPRLTAKVVGTDEYGVAINLESQAISCSCPAEKPCKHIAAVVLVALSESTETTPAAKA
                     VETNETYDLAVRMFEQGQVLLNGISASGHMIPISRNNFRFLRPAHRDMLSPIIGYIAH
                     VAERGMPFRDFAYTLLEKLARDSEVKFFFEMADQPTRCAGIVKPRSELHEIEDAADEV
                     HYEKFGYRRVRFERSHTVADPVTGDLFTFQHNMYHPMVRRGKDDNYFYVIAAPHDMTV
                     TPKGYQLENIERMRGGAMYRKWQHAFETMAAAGPRLMLSVMGVENQKTGKLAIEGRLE
                     FAYARDAADLKSRDAGAEIADGAKPVFGIAISTARHLTPPTEIVLHKTGVLVRRQLRA
                     EQALLLRPIPLQYTTQGRVRIAKKHYAEFFTEQVPAAKAAGLIVRVHDNILGILAKPL
                     ATLSITGSSGIDWFEGRIEVPGLEHGHLREAIKAYRRKEELVKLKNGNWLSVQGSGIA
                     DVLRSLDRLGLTANADGTIGKISFAQLASLEAEKELQLRTEAGAESAQQKFRSFIDTA
                     APEVQPAKTLRATLRHYQLDGFRFLLRLYEAHTGGILADDMGLGKTVQAIAAMDYLTP
                     RPLSTMWRGAGGEARKFIVICPLAALGVWESEIARFAPDLRVYRWHGPQRSEAEARNA
                     QVILTTFATFSLDAEKLCATEWALAFIDEAQFIKNFRSRSARELRKLNAQSVVCLTGT
                     PLENYLEDLWALFDLIFPGYLGTAQSFKDAYGGHLDLRDREGLLKKIRPFMLRRRKAD
                     VLTE"
BASE COUNT         3428 a         4829 c         4814 g         3727 t
ORIGIN      
        1 cgtgaagcca tgccactcta tgcgcaaggg ggaaaggcgc gtaaagccta tttgagcctt
       61 cccgcaacct cgcgaatcag cttgagcgtc gtcgcgtcgg tatcatagat actgtaccag
      121 ccctgcgctt cgtgcggtgg gtcaccgatg aaattgtcgc ccggttgcca ttgtacgcgc
      181 gcggggcggc cttcgccacc ccaggcccag aagttcacac ccgcaatcgg cgagtcggtc
      241 ttctgcgatt ccatcacgtg gtaaaatgct tgcgcgtaaa aatcgtctcg cagggtcacg
      301 ggagtgccgg cagtgaaacg gttcaggtcg cgtgaaaaac cgaactcctc aaacaccagc
      361 ggcttgttca gcttcttcgc cagctcgcgg tgctgcgcga gctgtttgtg cagaacttca
      421 actgctttgg cataagtttc gctcgccttc cacgggctgt agagccccga gttctgcacc
      481 cagatgtgca cggtcgtgta atcaatatct ttgtgggagt gattcttgat ctggtcattg
      541 cccgagaact ggaagacctc ccccattgca ccggtgctga ccaggtggtt gcggtcgagt
      601 gacttgatga gcgccgcaga ctcggcgatc cacttgtcat agctgtcgcc gctcagaaat
      661 ccggctggtt cgtttgcgag ctgccaggcc atgattgtcg ggtcgtcttt gtaggccctg
      721 cgcgtgacgg agttgatgcg ggtgacgagc atgcgcacag tgttgtggta gaagccgact
      781 gccacggggt tcttgtagaa acgggtgaca tagtagttgt aacgaaacca gcgccaccac
      841 gaagagagtt tgtagtcaga gccgccgcgc gcctcggggt cgaactcggg ataaggaatc
      901 tcgcctttgc cagcccagcg catatactgg ccgaaccctc ccgaccactg ccagtagttg
      961 ccgagcacga tcaccgcggt catatcgcgc ttcgccattt cggcgagcag gtagtcgagg
     1021 ccctcgagaa gtttccggtc gaaaatacca ggcgcagtct gcatcgccgg cacaatacgg
     1081 tacggttcgt tatcggggcc ttcggttgcg gcgagaatgc gcaggttctt gatgcccagt
     1141 ttctgcaggc ggtcaagttc gcgtttcagg cggggccggt cgtgcgcacc gaggttcatg
     1201 ccgtaccaga agttcgctcc catgaaggtg tacttcttgc cgcctcgcac cagatccatg
     1261 ccgcgcgttt gcacgaacga gggggtggcg ctgagcgttg tcaggagtga ggcagccatg
     1321 gcgatgaggt gaggcttcat gtgaggttta gcggcagggg tagaacttcg ccgcgaccgt
     1381 cgagtgttta cattactttt tgaccacaaa acactggtag caacggcaaa gatttgctat
     1441 aattgagtct caggcaactt catgcaaaac accgaaaagg ccgcatttac cggctacgaa
     1501 aaattcctca tcgcgatact cacgattctg cagttcaccg tgattcttga tttcatggtc
     1561 ttgtcgccgc tcggcgcaat actcatgccc gcgctctcga tcaacccgca gcagttcggc
     1621 tgggtcgttt cagcctacgc cttcagtgcc ggcgcctcgg gcctgctggc cgccgggttt
     1681 gcagaccgat tcgaccgcaa gcgcatgctt ctggtttttt acacgggctt tctagccggc
     1741 acactgctct gcggcctggc gcccgactac cacacactgc tcgccgcacg cgtcgtgaca
     1801 ggtatcttcg gcggcgtcat cagcgcgatt gccttcgcga tcgtgaccga cgtttttgcc
     1861 ttcgcggcgc gcgggcgtgt catgggtttt ctgcagatgg cgttcgcctc gagccaggtg
     1921 ctcggcattc ccgtagggct ctacgtcgcg aacaagttca actggcacgc gccgttcttg
     1981 ctcattgtcg gccttggtct acccgtggga gctgtgattg ttctgaagat gaaacccatc
     2041 gatgcacacc tcgcgctcaa gatcgacaag agcccattcc accacctgct caccaccatc
     2101 agtaaaccgc gttacctgcg cggcttcgcc gcgacgacac tgcttgctac cggcggcttc
     2161 atgctcatgc cgtttggcag cgcgttcggc acgggcaacc tgggcctgag caacgacgac
     2221 ctgccgaccc tctacatggt caccggcatc acggccatga tcgccggccc gatagcaggg
     2281 cggctcagcg acactatcgg caagtacaag atattcacga tcggttctac ggtcggcatt
     2341 atactcgtac tcatctacac gcaacttggc cgcagtccac tctggctcgt gatgctgatc
     2401 acggctgcgc tgtttgcctc catcaccgca cgtatcgtgg gagcttctgc gttaatgagc
     2461 ggtgtacccg caccgcagga ccggggcgcg tttatggggg taagctccgc ctttcagcaa
     2521 atttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaccg caggaccggg
     2641 gcgcgtttat gggggtaagc tccgcctttc agcaaatttc aggtggcatt gcctcggcgg
     2701 tcgcaggtgc aatcgtcgtg aaaacaagca gcggtgcact cgcgcgctac gatgtgctgg
     2761 gttacgtagt cgtcggcgca atgctggtga caatcatcat gatgtacacc atacaccgtc
     2821 tggtcatgca ccaggaccac aagggctaac acatacatgg ccgaaaagac ggcagccaaa
     2881 cgcatcggcg ggctcgacgc gctgcgcggt ttcgcgctgt ggggcatctg cgtcgtgaac
     2941 ctgccgctca tcgcccgctc gtgggattca taccggcagc tgcccgagac cgcgagcgca
     3001 cggggtgcgc tgtttgtgaa ctcactgctc tttgaagcaa aattctttac gctgttttca
     3061 tttctctatg gcatcggtat tgcggtgcta cagcaccgcg agggtacggc cttcttgctg
     3121 cggcgctttt tgggcctgat cgtcgccggc tttctgcacg ctatattgct gtttcccggt
     3181 gacattctgc tgagctatgg cctgctgggg cttttctttt tgccgctgag ccgcatgcac
     3241 aaccgtgcgc tgctggtgac agcaatattc tgcctggcga tttcaggcat gacgtacaca
     3301 tttctgggtg tgatgagcca ggcgaatgcg gcgattccgc cgacgaacta taccgcgtct
     3361 tatacagcag tcgtcgcgag caacctgcaa gtctaccccg tatcgctggg ttatgtcatg
     3421 ctcttcaact ggccgggggc gctggcgatg atctgcctgg gttaccttgc cgaacgccgg
     3481 caatggctgg ctaaaattcg cctgaccgcc ttctcgagcc tgtttgcgct cgcgggcgtg
     3541 acgggcagcc tgctctacgc gagtgcagct tcgtggcaac tgaagcaata catgcctgtc
     3601 ggcatgctcg cgatggcgct caccgctcca ctgctgtcgc tcttttacgc gcaggtgata
     3661 ctgactcttg cagaaaaaac aaccgcatgg ctgacaaagg cgctcaccgc ggcgggcagg
     3721 ctgtcgctca cgaactacgt gacgcagtca ctgcttgcag gtattatctt tcacggctat
     3781 ggcttcgggc tctacaacaa gctgagctat gacggattgt ttcttgtggc gacgggcatt
     3841 taccttgcga cgatcggctt ctctttgtta tggttgcgct acttctcaac cggcccgctc
     3901 gagtggtttt tgcgctcgct ctcgcactgg cagctcatgc cgatgcgaag tgcaggttag
     3961 gccggctctt cggcaaccgc gtcgatgtct tcgttgcgcg acagcagcac catcatgcca
     4021 cctgcagaaa tatcgctgag atcggcgaca agggcttcgg ggtgctttag atgcactttg
     4081 cagtcgaggt gcggcccaac gagaaagcgt tcgtatttgc ggttttcatc ggcctgcatg
     4141 cgcttgtcct gagtgggcag ggtagctgac gaacctgtta tgccaacaga attttggatg
     4201 cctggctatt tctcgggcac cggatcatcg ccgccttttg caaagggctg gcagcgcaaa
     4261 atacgccaga gagtcaggta gaaggcgcgg tggattggca gcttttcgaa tgcctcaacc
     4321 gcgtactgcg agcaggtcgg atgaaaccgg cactggccgc cgagaaacgc ggagaacgtg
     4381 tactggtaga agcgaatgag gaagataaag aattttctaa tcgcggtatt cattgtgtat
     4441 ttccaggatt tcgggcaggc atgaatcgag cccggcaagc aggtcggcac ctttctgtgg
     4501 gtgcgtcttc atcacctcga actcctcggc ggtcagcttg cctggcttca gcaaaatgtg
     4561 gtcaggaata ccaatcttgc caacatcatg catcggtctt gccagggttg tttcaagcag
     4621 cgtcagattg accggagagt catctgcaat aagaatcgtt gccgcagaca cagaaaaagc
     4681 gattcagttt atgactctac ggtaaacgtc acaccagaat atttctgcag gcggtcgacc
     4741 aacggggctg caatcactcc gcccggtgtg aagacaccgc caccagactt tgtctcaccc
     4801 aacgcgagcg cggcctgcgc aatcatcttg ctcgttgagc catagcctgg gtcacggtcg
     4861 cccttgaccg caatctgcaa gcgacgaccg tcagggtatt cgccaatatg aacaatgtcg
     4921 taaaaaccgg tttcgcgctc ttctttcgaa ggaccttcgc cgggtttaag ccccttgccc
     4981 gcgaacgggt tcatctttgc ggccgcgtca accagcgcac tcgccgcttc gccgagcgtc
     5041 gtgtacatca tctcgtcgta gacaaagtct ttgccgtacg gatggcctag cagaaagttc
     5101 gtgcggtgca cgttcttggt attgatcggc gccataacga acggcaccac ccagacgccg
     5161 gccgaacgat cgtactcggg caccatgccc gatggttgcg ccggcccttc aaaacctgga
     5221 gtcagtgcaa acgggctacc cattatcttg agcagcgacg ggttcttcat accggcgcgc
     5281 atgacttctt tcatactcgc cagcgtaccg cctgaagctc cccctttcat cttgcgcacg
     5341 cggcctttga cccgcggtgc aggtgcgcca aaacgccgga tggcttcttg ttgcagaaac
     5401 cagacaccca ggtcagaggg aattgaatcg aatccggcag agatcacgat gcgcgcaccg
     5461 ctttttttcg ccgcgtcatc gtacgtttcg atcatctcgc gaatccagtt gggttcaccg
     5521 cagaggtcga cgtagtcagt gccagtctca gcgcaggctg acacgagcgg ggtaccgtag
     5581 agctggtacg gcccgacggt ggtgaggaca actttcgtcc gcgcgcacat tgccttgagg
     5641 ctcgccggcg acgaagcatc agcttcaatg agggggattt ctttgggtgc gccgatcagg
     5701 tcgcgcactt cggcgagctt cgcgcaattg cgccccgcca tcgcccattt gggtgctgag
     5761 cctgtcgaag catgcgagag gtattccgcc acaagacggc cggtgaagcc cgttgcgcca
     5821 tagacgacat cgtcgaattc tttgtgtacc atgggttaca aaagaaggcg aaaacccaac
     5881 gccaagctaa ttcaccttct gtgaccttct ggtctgggcc atggcgcgca gtttaccatc
     5941 ggaaaagatt gacgcacccg atttttatct gaacccgata atctgacctt cctggcagtt
     6001 cccaaggacg attttctctg cctgaagccc gaaaatagcc gtgcagcata tcacccgcac
     6061 catctggctg gtttctttag tctcgctgtt caccgacatc gcgagcgaaa tgctctaccc
     6121 cgttatgccg atctacctgc agaccatcgg attctctatc gtctacatcg gcctgctcga
     6181 aggaattgca gaggcaactg caggcctgag caaggggttc ttcggccgct ggtccgatgc
     6241 aacgggtaag cgtgtaccat ttgtccgtgc gggttatttg ctcagtgcgc tggctaaacc
     6301 gctcattgtg gccgcagcgc agccgctatg ggtcttcttc tgccgcacgg ctgaccggct
     6361 aggcaagggt gtgcgcaccg gcgcacgcga cgcattgctc tctgccgaaa ccgacccggc
     6421 acacaagggc aaagtgtttg gttttcaccg cggtatggat acgctcggtg cagccatcgg
     6481 ccccgcgctg gcgctgctgt ggttgtggca aaacccgggc cgataccacg agctgttcct
     6541 gtttgcgttt ctgcccggcc tcatggctat cgcgctgacc tatctgctca aagaaaaggc
     6601 ctcgcaggca aaggttgcca ggcagtttcg ctggtgggat tttctgcgct actacacctc
     6661 ggccagtgtg gcataccggc acactgtcaa aggactgttg cttttcacgc tgttcaacag
     6721 ctccgatgtt tttctgctgc tcgttcttaa atggcggggg ttcaccgacc aacaggtgat
     6781 cggcgtctat attttctata acctggtcta tgcagtgttc tcatacccca tggggcgcct
     6841 cggcgatacg attggcctta aaaaaacgtt tcttgcaggc cttcttgtat ttatcctggt
     6901 ctatactgga atcacctttg cacgcgactt ctacgctcta cttaccctgt tcttcgctta
     6961 tggcatctac gcggcgatga cagagggaat ctccaaagcc tggatcacca acctctgtaa
     7021 caaagaagat accgccactg cagtgggtac ctatacagca ttcgccagcc tggccacgat
     7081 gctcgcgagt ttcgccgcag gactcatttg gaaattcgtc tcacctgaag caactttcct
     7141 ggttgctgca gcgggtgcgg ggattgctgg ggtttatttg gtgttaaccc ctcccccagc
     7201 ccctcccctg ttagaagagg ggaggccgta ggcggggtgg ggtttgcaaa taatgaaaac
     7261 caaactcctc accattctcg cgctctttac ccgtctcggc gtgacggcgt ttggcggccc
     7321 ggtcgcgcac attgcgctga tgcggcatga atttgtcgcg cggcaccgct ggctttccga
     7381 tgagaaattt gtcgagctga tcagcctcac gaatctgata ccggggccga acagtaccga
     7441 gatggcgatt cacctcggct atgaacgcgc cggccgacgg ggcagtgttc tcgcgggact
     7501 gggcttcatc gtaccagcag tgatcatcac actgggcata gcgtggttct acggtgaata
     7561 cggcaccctg cccgcggcgc gcgcactgct gttcggcatc aagcccgcaa taatcgcggt
     7621 tatcgtcttc gcggtattgc ctctcttcaa gagcgcggta aacacactcg gaaagcttgc
     7681 tgctgcggcc gcgaccgtgg tgttgtgttt cctgggtatg agcgaagtgc tcgtcatgct
     7741 cggtgtggga gtgttctttg ctttggcaaa atgcgccgca accagggcaa ctacgcgggg
     7801 catgcaaggc cttgttccct tttttctact gcagccgttt tttaccggta ccgcgacggc
     7861 cctgaatacc aaattgttcc tgatttttct gaagatcggt tctatactct atggcagtgg
     7921 ctatgtgctc ttcgcctttc tcgacgccga acttgtcacg cgcgggttat taccgcgttc
     7981 acagctgatg gacgctgttg cggtcgggca aattaccccc ggcccggttt tttctgcggt
     8041 aaccttcatt ggctaccagg tgaacggctt cagtggggcg attgtgtcca cggtggcgat
     8101 ttttctgcca tcgtttattt ttgtcatcgc cacgcattcg ctgttgccga ggctcaggaa
     8161 atccacactg ttctctgctt tcctcggcgg agtcaatgca gcctcgctgg cgatcatgtt
     8221 gtttgccctc gtcgcgatgt cacgcgatac gttcacgcac tggtcggcct tcatgatcgc
     8281 agcgctgtcc ggcgccggac tctatctctt caaaaacccg ggtagcacct acgtggtgct
     8341 attcggtgct gctgggggca ttgtattgtc gtactggggg atagtttaac cgacaaaaga
     8401 gcccagcttc tcgcgaagtt aagttcgttc gcttcttgcc aatacacctg ctatcacgtc
     8461 atgcccgcgc aagcgggcat ctatattcgt atgtactgcg agctgattac ccgctaaccc
     8521 gctttttcag aaattcccgt agctgttcat attccatcgg gcgcgcgtag agaaaaccct
     8581 gcaggtaatc gaccccttca tgctcgagcc aggccgactg ctcggcagtc tcgacaccct
     8641 cggcgacaag gtaaagctgc agcgcgcggc ccagtgcaac aatcgagcgg acgatcgact
     8701 ggtcgttgac cgagtgcggt aaaccgtcga cgaacgactt gtcgattttg attttttgga
     8761 cgggcagcag cttcaggcgc gagagcgatg aatagccagt accaaaatcg tcgacgctga
     8821 tgcctatacc catgtcgcgc aggcgggtga gctgctcgtt aatgaggtca aagtcacgcc
     8881 cgatgagttc ttcgagtacc tcgacctcga gtatatgccc tggcaaaccg tgctgctgca
     8941 gaagattttg gatttcgata gcaaggttat tgttatagag ctgctcggcc gccaggttga
     9001 cagagacccg gccaacatcg agaccatcgg ccaaaagatc cgccacctgt ctgcaggcct
     9061 gccataacac aaatgcaccc agttcgcgcg tcttgccgat ttcggtgacg agcggcatga
     9121 accgcgcggg gctcagcaga cccaaccgcg ggtgctgcca acgcaccagt gcctctaccc
     9181 cggtgtaaag cgtacgccgc gcgtcataaa ccggctggta ccacaggaca aactgctcct
     9241 gctcaagccc tcggtgaatt tcatcgacca tacggctttt gtcctgcagc tgctggtagc
     9301 cgtgctcaga gaaaactccc acaccgccct tgttttcctg tttggcattc ttnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagccgtgc tcagagaaaa ctcccacacc
     9481 gcccttgttt tcctgtttgg cattcttcag cgcaatttcg gcggcaagaa tcgggtcggg
     9541 tactgctttc tggtcggcag tgtgaaaata cacggcagtt gtaccgctga cacggacttc
     9601 aacgccgtat tccgtttcta tggcgtcttc aagaatctta cggatagcac tcatgaattc
     9661 atcgacaacg tgctgttcgg gcagcggtga gagccaaaag cccagcacgt caccgctgat
     9721 gcgccccgaa gcgctgccaa agcgtcgggt aatttcgctg gcacgctcgc ccagcacacg
     9781 cagcacactg ttagcgataa cctggccgaa cttatcgttg atcgccgtga acgcatcgag
     9841 accgagcagc gctagcaggt gttgctcatc tggtttagct gcaatatcgc gaaagacctg
     9901 ttgctgaaac gagcgccggt tcactaatcc cgtaagactg tcgtattgct caagaaactg
     9961 gttatagcgc cgctcttctt cccacaggtg ttgctcctgt atcgcagaaa cggcgtcggc
    10021 tgccgccgcg gcaaaagaga tatcgagcag cgaccaggta cgcaccgccg cctctgcttc
    10081 gatacagaga ataccggcaa gtttaccgcc ggcgaatgtc gggcagtcca gcagcgaata
    10141 gatacccaat ggcttcaggt aagtctcggt caaatcggcc gtgcgcgggt cacggcgcgc
    10201 atcgtcggca ttgatgatgc ggtcgcgccg catggcctca aagtagcccg ggtatctggc
    10261 cgccgtaagc tccggtccct tggtataatg gtgtttgccg cgctcgtact gcagggtgca
    10321 ggtaatcgca tcaccggctg agttaaggcg ccacaacccg gcccgcgcaa catcgagctc
    10381 aaccgcgcag tagtggagta tcttttggag tttttgttca gccgtgagac cgtggctttc
    10441 ctggccgtag cgaatctgca tcagccgctg gtggcgtgcc gcaagtgaga ccaggtttgc
    10501 catatcgaag ggcatcaggc cattgttgag gcaggggggc tggcacgtaa ataggaatca
    10561 cagaagccct gcatttctac gggcttatgc ctgtagcgat tacttcctat ggcctgtatt
    10621 tttagcgggt accatacggc gcagcgcctg ccgcaggtcg tcgcaaagtt cagaaaagtc
    10681 gagctccgcc acttctttgc gtggtacaat cgcgatatca aaaccagcag gattcgagcg
    10741 cccttctttg agcgttaaaa agagcgcctc gcgcaggcgg cgcttgatgc gattacgcac
    10801 cgttgccggc cccagcctgc tgctgacagc aaagaccacg cgcagaggtg ggttagtatc
    10861 tgtagtattg tcaaagcgca gcatcaggta ccggccgaaa gactttcggc cgttcttaaa
    10921 gaggtgatcg atgcggtttt tgagtttgag agaagtgaag tagcgggaac gcttgagggg
    10981 aatcggtgtc tcagacaccg attagcgttt aacgatatcc cagcggcggc gctcgtcaga
    11041 aactgtcagt ttccagcggc ctttttggcg gcggcggcgc aggacttcac ggccctcggc
    11101 tgtcgcattg cgcgtacgga acccgtgtgt gcggttacgt ttcttgtttg cgggctgcca
    11161 tgtccgtttc atgggtgcaa atcggggcgt gctgacaggg tgtaaagcgt aagtgtacag
    11221 tgacagcaga agctgcgctg gaattccata gccagacgac gcgactgccg ggcgagcatt
    11281 tcttgattat gtcacatcaa gtgattgagg ctgttgtggg ggtggttaag cattcagaaa
    11341 ctcgtcaggc gaaatattaa gctgtttgag tatggccgta acgtaccttc tggaagtttt
    11401 tcaatatgta ggtcgataag gctttccata ttttctccgt ccagttatgc gttaacaggt
    11461 tcctttatac gcaacaagtt acaggaaatc aagacaaatt cgctcgacgc cgtgctcgcc
    11521 ggccaatgcc gttctccgtg tcgacagact attttgccct ctttccgctg gtgatttttg
    11581 ctatcgttat tgcgatcttt gtacgcttct accggcgcaa agaccagctg cacgccgccg
    11641 agttgggtaa gaagcccctg ttcagtgaca cgtgcggagg ggttgtcggc tggttaaact
    11701 acaagggacc tttcatcagg gttgccgttt atcctgactt cgtggtcgtc agctgcgaca
    11761 ggcagtacta tctgccattc ggcgagatcg ccagcatcga acggcggagc ttcctgttta
    11821 cgaagggtta ccgcatccac cacaggaatc cacagtaccc gaagcgcatc gaagtgtggc
    11881 tcggcaagca ggagagtttt gaggccgcgc ttgcaggaaa ggtaaccctc gtatgattcc
    11941 tggcgagccg attggccgca agtatgaccg tatcgcgcgc tggtggcacg agacgcacaa
    12001 agactcgcag tatggcattc cgcagattgc gcgggcgatc agctactgca ggaacaaggg
    12061 ccgcgcgctc gatatcggct gcggcagcgg cggccggatt tttcgcctgc tcggcgacca
    12121 cggtttcacg atcacaggtg tcgacgcttc ggcagagatg atcgcactcg ccggtatgga
    12181 acacccggag ctgcacctgg tgcataccga catccgcgaa tggtcaatcg acgaagcgtt
    12241 tgacctgatc atcgcctggg acagcatctt tcacctcgaa gaatacgaac acgaagaggt
    12301 gatcacgaag ctggtcgatt ttctcgcagc agacggagtg ctcatctatt cgctgggcga
    12361 cgccgtgggc agccatgaat cagactggca cggcgacaag tttccgtatg gctcaatcgg
    12421 catcgacgcc aaccttcgcc tgctcatgga tctgggctgt cacatccgcc atgtcgaact
    12481 cgaccagtac ccgcagcagc acgcttacgt tatcgcgcag gcgggtgaag gtttggaaag
    12541 agttgggtga ttaagttagt tagaacactc gagcggtgtg ggctgccagt cgcgttcacc
    12601 ggcgttcttc catttgccgc gcaggttggt cggccggttt gtcttgcgct cgatcgatgt
    12661 catgagatca aatgtaccgt gcgttgagcc gccggcctcg ctccatgtgc cggtaaaccg
    12721 ggagttcttg agtgtgccgg taatcttgcc atcgggcatc gcgacattat cgggcggcac
    12781 caccaggttc atctctgttg gctcagcggg tgctttgcct ttgctttgcg cgcccaggcc
    12841 cggccccagg acaacgattg cagccgcaca gacgtgcagc aggcgcctcg gttttgcgat
    12901 aatttttttc atgtgggtta tcatgacaat gtggcaatga gcgtcaggct gagggtgaag
    12961 caggcgagcg cgaccatgct cagcagaata tcctgcagtt tcatggcttg cttgtagatg
    13021 gctttatggt tgtgcatagt atctctgggc tttcgccgtt cgatactggg ttagacgttt
    13081 aggcagcctg ctgtacgaca aaaatccatt tgctacgaac cctccttata taaggttgac
    13141 gggcgcaggg gcggccaaca ggcgtgtgtg ggagttcctg cgttttcggg ccaggcacgt
    13201 aagcgaagca gctcccctga ctaaaatgcc aaaacccacg acaaaaaata cactgaacgc
    13261 caaagaaagt gcggacctgc tcgcgcaact gaaaacccga tttgaaaaga atatggcgcg
    13321 gcacaaaggt ctcacctggg ccgccgttga aaagaagctc gctgcgcaac ccggtaaact
    13381 cgcaagcctt gcgcagatgg agcagaccgg cggcgaaccc gacgtcgtat cgcacgataa
    13441 gaagacaggt acttttgtct tcatggactg cgccgcagaa agccccgcag gccgccgtag
    13501 cctttgttat gaccgcaagg cactcgatgc gcgcaaagag aataaaccca aaggcagcgc
    13561 gctggaggcc gccgaacaga tgggggccga attgctcacc gaagaagatt accgtgcgct
    13621 gcagaaactc ggggcctttg acctgaaaac ttcgagctgg attctgacac cggagaaaat
    13681 acgcaaactg ggtggagcgc ttttctgcga tcgccgctac gatcaggtat tcacgtacca
    13741 taacggcgca gaatcatact acgcggcgcg cgggtttcgc tgtgtgctgc gggtctgaat
    13801 gattgccaaa aaattgtccc tgagcgtcac gctggcggcg gcaattctgt cgaccgcctt
    13861 ggttgcacag aaagccggta gcgaagataa agcgcgcaag ccgcttctgg gcgtcgtgca
    13921 cacgcaggca cctcgcggtg tgcgcgttga gaagatcatg cagaaatctg ccgccgaacg
    13981 tgccggcttg aaggcaggcg atatcatact ctcggtgaac agtgtcgcga ttcgcgatcc
    14041 gaagcatctt ttcaccgaag tgcagcgcta cgcggcgggc acagtcgtcg cgattgaata
    14101 ccagcgtgat ggcaaaacac aactgctgca ggtacccctg ggcgagcggc tcgacgccgc
    14161 gtcgctcgca ggccagactg cagcggcttt ctcgctgcct caactcggca agaagactcc
    14221 ttatgcactc gcgaccggca aggttgtggt gatcgacttc tgggcaacct ggtgcggccc
    14281 ctgtgagcca gtcaggcgag cgctcgaaga gttccagcgt gaacgcagaa catccgacat
    14341 tgccattgtt ggcatcacca atgaagatga agccaccgtc agggcatttg tgcgcaaccg
    14401 gggtgcgcgc tacccgctgc tgatcgacgc gaagggcggc gttgcactcg actaccacgt
    14461 cgcggggtac ccgacgattg tggttatcga caagaaaggt attgtgcgct tcgccggctt
    14521 tgcctcgggt ggtggtctcg aacacgccct gcagctggct gaaaagctgg ctgcggaata
    14581 acgcgggccg catggccgaa cagcaactga caaaaaccga agcagctgcg ctgcatggca
    14641 tcatcgacga cgtgagtccc gtaacgctgc gccgcgcgca ggattacgca cgctcgggtc
    14701 atgtcggccc gatcgcgcgc aaggggccgc ggctcaccgc gaaggttgtg ggtaccgacg
    14761 agtacggcgt cgccatcaac ctcgaatcgc aggcgatcag ctgtagctgt ccggccgaaa
    14821 aaccgtgtaa gcacatcgcc gcagtggtgc tggtggcctt aagcgaaagc acagaaacaa
    14881 cacccgctgc gaaggctgtt gaaaccaacg agacgtatga tctagctgtg cgtatgttcg
    14941 agcaggggca ggtattactc aacggtatct ctgcgtcagg gcacatgatt ccaatttcgc
    15001 gcaacaactt caggttcctg agacccgcac accgtgacat gctttcgccg attatcggtt
    15061 acatcgcgca tgtcgcagaa cgtggcatgc cgtttcgcga cttcgcctac accctgttag
    15121 agaagttagc gcgtgacagc gaggtaaaat ttttcttcga gatggccgat cagcccaccc
    15181 gctgcgccgg tatcgttaaa ccacgttcag aactgcatga aattgaagac gcggccgacg
    15241 aggtacacta cgaaaaattc ggctaccgcc gcgtgcgctt tgagcgcagc cacacggtcg
    15301 ctgatccggt tacgggcgac ctcttcacct tccagcataa catgtaccat ccgatggtgc
    15361 gccggggcaa agatgacaac tacttctacg tgatcgccgc gccgcatgac atgacggtga
    15421 cgccgaaggg ttaccagctc gagaatatcg agcgcatgcg cggcggcgcc atgtaccgca
    15481 agtggcagca cgcctttgag accatggcag cagccggccc gaggctaatg ctttctgtta
    15541 tgggcgtcga gaaccagaag accggcaagc tagcgatcga gggccgcctc gagtttgcct
    15601 atgcgcgcga cgctgccgac ctgaagagcc gtgatgcggg ggcggagatc gccgacgggg
    15661 caaagcccgt attcggtatt gccatcagca cagccaggca cctcacgcca cctaccgaaa
    15721 ttgtgctgca caagactggc gtgctggtca gacggcagct gcgcgcagag caggccttgc
    15781 tactgcggcc gattccgctg cagtatacca cacagggccg cgtgcgcatc gcaaagaagc
    15841 actatgcgga gttttttacg gaacaggtgc cggcggcgaa agcggcgggt ctcatcgtgc
    15901 gcgtccatga taacatactc ggcattttgg caaagccgct cgcgacgttg agcattaccg
    15961 gttcttcagg tatcgactgg tttgaaggca gaatcgaggt gccgggtctc gaacacggcc
    16021 acctgcgcga agcgatcaaa gcctaccgca gaaaagaaga actggtgaaa ctgaagaacg
    16081 gcaactggct gtcggtacag ggttcaggca ttgccgacgt tctgagatcc ctcgaccggc
    16141 tggggctgac cgccaatgca gacggcacca tcggcaaaat ttcgttcgcg cagctcgcga
    16201 gcctcgaagc cgaaaaagaa ctgcagctga gaaccgaagc cggcgccgaa agcgcacagc
    16261 agaagtttcg cagcttcatc gacaccgccg cccccgaggt tcaacccgcg aagacgctca
    16321 gggcgacgct gcggcactac cagctcgacg gcttccgctt cttgctgcgc ctctacgaag
    16381 cgcacaccgg tggcatcctg gccgacgata tgggcctggg caagaccgtg caggcaatag
    16441 ccgcgatgga ttacctcacc ccccggcccc tctccacgat gtggagaggg gctgggggtg
    16501 aggcgcgaaa attcattgtg atctgcccgc tggcagcact cggcgtctgg gagtcagaaa
    16561 tcgccaggtt cgctcccgac ttgcgtgtct accgctggca tgggccgcaa cgctcagaag
    16621 ctgaggcccg caacgcacag gtgattctca ccacttttgc cactttctcg ctcgacgccg
    16681 aaaaactctg cgcgaccgaa tgggcactgg ccttcatcga cgaagcgcag tttatcaaga
    16741 attttcgcag ccggtcggcg cgcgaattac gaaagctcaa cgcgcagtcg gtggtctgcc
    16801 tcaccggtac accgctcgaa aactacctcg aagacctctg ggcgctgttc gacctgattt
    16861 ttcccggcta cctcggtacg gcgcagagtt tcaaagacgc ctacggtggc cacctcgacc
    16921 tcagggaccg tgaagggtta ctgaagaaaa tccgcccatt catgctgcgc cggcgcaagg
    16981 ccgacgtgct gaccgaac
//
LOCUS       DAOQJF010000020        16901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1615_length_16901_cov_5.088211, whole genome shotgun sequence.
ACCESSION   DAOQJF010000020 DAOQJF010000000
VERSION     DAOQJF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 16901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16901
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1615_length_16901_cov_5.088211"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..803)
                     /locus_tag="PLF85_02055"
     CDS             complement(195..803)
                     /locus_tag="PLF85_02055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNE18467.1"
                     /translation="MQASPFTAHPWHGVARGSEAPAVVTAFIEIVPSDTIKYEIDKAS
                     GLMKVDRPQKYSSLCPTLYGFIPQTYCDENVRQLARDKKVVRGDRDPLDICVLTERSI
                     TGNGFLVRARPIGGLRLIDDEEADDKIISVMAEDPLYANIHSLKEAPQALIDRIRHYF
                     LTYKDFPGRKQKVEIGAVYERDEALNVINASILDYDSKFRGA"
     gene            1577..3118
                     /gene="guaB"
                     /locus_tag="PLF85_02060"
     CDS             1577..3118
                     /gene="guaB"
                     /locus_tag="PLF85_02060"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNE18468.1"
                     /translation="MARSSSSKSSSKGYQDGFSASEIFEKDQGITYKDFLILPGYIDF
                     APQDVNLETMVSRNVKTKIPLISSPMDTVTESKMAISLALLGGIGIIHNNLSIEDQAA
                     QVERVKRYENGFITDPIVLSPKHTIADIDNIAERYGFSGVPITEDGTLRTKLVGIVAN
                     RDVDFERDRSIPLAKVMTTELVTATKGVSLLQANEILRKSKKGKLPIIDKEGRLVALM
                     SRTDLVKNKEYPLATKDAQKRLRVGAATSTHPHDRERIDMLIARGVDLLVIDSAQGYS
                     KFQIDLLKELKKKYPEVDVMAGNVVTREQGEALIKAGADALRIGMGPGSICITQDTMA
                     CGRSQATAVYYTAQMAAKYKVPVIADGGIANIGDIAKALAVGGSAAMVGSLLAGSKEA
                     PGEYFYENGVRLKKFRGMASIEAMEGSGGGAKRYNVEDQKIKVAQGVSGAVVDKGSLF
                     EFIPYLMQGVRHALQDMGCRDIASLHKALHTRKLRFEPRSLAAQAQGGVHGLYSYKKP
                     IIGAD"
     gene            3130..3735
                     /locus_tag="PLF85_02065"
     CDS             3130..3735
                     /locus_tag="PLF85_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNE18469.1"
                     /translation="MKLYGSITSPFVRRVRFLCHQLGQSFEMVDSLTEEGQNAMRDKN
                     PIWKVPCVEIDDLVIWDSHTIIDYLTEKYKGNPKLLRSPDGNEYWRERNLVTAADGCV
                     ESAINVFYLKKDGVAVLDVAYLKKQMARVESILTWLKSQLYGHYFTVEEHIGMSELTL
                     YCILDWLRFREMYPVNEDPVLKGFLEHHNSNPGFVATKLPG"
     assembly_gap    3865..3964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4071..>5194
                     /locus_tag="PLF85_02070"
     CDS             4071..>5194
                     /locus_tag="PLF85_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE18470.1"
                     /translation="MIRFVVTAIATLLISGSVFASADGHHDPVHDLLMHIGLTILVAT
                     ILAYVGHVAKQPLILAYLAAGALIGPNFGLGLIKTEKDISTISELGLVLLLFMIGMEI
                     DLKKIKASGKSLILTGITQFAICAGLGILFFQLIGFQLGGGKYDAFYLAVCCGISSTA
                     IVVKLLYGKFELDTLAGRLTLGVLVFQDIWAIVVLGVQPNLANPEVLQILLSFAKAGV
                     LVAISVAMSKYVLPALFSRIAKIPELTLLMSVGWCFAVCAAALGFGLSLEMGALIAGV
                     SISTFPYNLDVVAKTITLRDFFITIFFVSLGMKIPNPVTHINLVGIAVIVAAFVILTR
                     FLSIYPVLFSLKNGNRVSILTSLNLSNVSEFSLVIAALGLA"
     assembly_gap    5195..5294
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5295..6091
                     /locus_tag="PLF85_02075"
     CDS             <5295..6091
                     /locus_tag="PLF85_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE18471.1"
                     /translation="NLSNVSEFSLVIAALGLASGHISSDILSIIIFTFVITSIFAPYM
                     IKYNQQLQGKITGVFEMIGLRDKQKFVEPTEQELNKDIALLGFFKVASSFVTDAVEDT
                     GKNSLKEKIVVVDFNQTVHSKLKSLGIPAVYGDIGHIDTLHHVGLHHTKVAISTIPDT
                     ILVGTSNTKLIKQIEDIAPHAKIIVTAENPESALKMYEAGADYVFLPRQLASAHLMPI
                     VRDMLATTEEGNSPAAKSRRKKAEAKLEQLREDAISELRERHEIIA"
     gene            6149..7780
                     /locus_tag="PLF85_02080"
     CDS             6149..7780
                     /locus_tag="PLF85_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNE18472.1"
                     /translation="MIRQDNTYKRYFDALLLGFTIFAAIEVPLHLALQYPKAGWRQII
                     GLVYPVIFTVDILVTFFTTITVDGEEITSRKEIAVRYLRSWFIVDFLAATPFDLLFAE
                     GWLSELSNAARSLRLFSPRYIRILLMVRMLRIYHILPFLQRTSKKDLLNPGIVRLIFT
                     VFIVLVIAHWISCGWIALGKIDEKADAVTNYIRALYWTITTITTIGYGDITPTSNIQT
                     LYTMVVQLIGAGMYGYIIGNLASLLANSDLARTQFRAKLEKVQTFLQYRDVPQDLQDN
                     IRTYYDYLWNNRRGFDESAVLQDLPQSLKLQVALHLNRDIIEKVPMFKGAPDELIRQI
                     VLNLRPLLFTPGDYIFRKGEMGDQMYFISRGKVEVVSEDGKTVYATLGDGAFFGEIAL
                     LFSSERTASVRAAEYCDLYTLDKYTFDNVLSKFPGVAEHVKIMAEERRQRTIAERSDA
                     TAVSPEKAVIENVQVSYQSGRVLVDWPDVPGASGYQVTRWDDQAERWVYISRSLLLSE
                     CHDVLPLRRQTNTYRVRAIIGDAFGPWSKAVHVNA"
     gene            complement(7790..8425)
                     /locus_tag="PLF85_02085"
     CDS             complement(7790..8425)
                     /locus_tag="PLF85_02085"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE18473.1"
                     /translation="MINIDGIVHAIQSIHEPWTYLIVFGILLLCGFGLPVPEDITLIA
                     GGLMAYYGKANVFVMIGVGLAGVMLGDGAMFQIGRRFGYRVFEWRWMSKIMHAERLES
                     VKQNLQNHGYKVIFSARFMPGVRSLVFLSSGALGIPFRVFLLFDGLAAMISVPAIVYS
                     CYFFGDQINNAVGVIKNVEQAIVALIGLGILYFLVKYFVKKIRQAKSTEET"
     gene            complement(8462..8875)
                     /locus_tag="PLF85_02090"
     CDS             complement(8462..8875)
                     /locus_tag="PLF85_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18474.1"
                     /translation="MQRHELEAFTTWRTPEGIIRTEAKPGIDIELRHAVENSLVVNEL
                     CRGRLRPLLINLRHLQSISSEARAYFAARDRSSFVNAFGFLVHSRFQKMVGNIFIQFN
                     RPRVPVRLFNDEQEAISWLSRFLPEEQGRSLPGRN"
     gene            8977..9336
                     /locus_tag="PLF85_02095"
     CDS             8977..9336
                     /locus_tag="PLF85_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNE18475.1"
                     /translation="MKTISKLLLSVFLATFALSAEKPEGKPGRKGPAGMPGRMLEMMD
                     TNKDGKVSKEEWQAHHDQRFTEMDTNKDGAITSAEVKEHHGKMREKNAEMCPDCQRGK
                     KGKHKGHHKKPADDDND"
     gene            9348..10049
                     /locus_tag="PLF85_02100"
     CDS             9348..10049
                     /locus_tag="PLF85_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNE18476.1"
                     /translation="MDAALPQDNPGAMERLLDSAYGQVLNGFGVFTPADRLAQEYLAQ
                     NAGDARRAAADLIGWQMTKAAASGFVTNLGGIGTLPVAIPANVSSVIYLQLRMVAAIA
                     IMAGYDVRSDKVRTLIYVTLLGSAAAEVLKEAGIKLGQRITTAAIMRLTGQVARRMTS
                     SAAARLMARLGLAQAGNLARLVPVAGGLIAGATDAAATKAIGLVATQVFLPVTEENPT
                     GSDVTPGNYPPEQIG"
     gene            10080..10757
                     /locus_tag="PLF85_02105"
     CDS             10080..10757
                     /locus_tag="PLF85_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNE18477.1"
                     /translation="MKVYIAEIIGTATLVLFGCGSAVFAGASIGIVGISFSFGLALLA
                     MAYAIGPISGCHINPAVSVGAYIAGRLSGRRLVGYVIAQSLGAIVGAALVYAIALGDP
                     AYKLAVNGLGQNGFGPGYQGGFNLESAFLFEAIATFIFLVVILGSTSSGAAKKFAGIA
                     IGLSLVLIHLVGIRITGVSVNPARSLGPALFVGGQAMAQLWVFIVAPLLGAAFAGLMY
                     RGRVLED"
     gene            10769..12607
                     /locus_tag="PLF85_02110"
     CDS             10769..12607
                     /locus_tag="PLF85_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18478.1"
                     /translation="MSFFRACCAAALLSAIAAIYAQKLDPEAANKPAENAEKVKLAKP
                     EDVTTDEKGSEVATSTAREALLPARKLKSDVDKEVDDLPFGVPQIDKRENVFGDKQDK
                     DAILKRMEYQIGFIAEGAGFNNTDLRRLDESTVATIQNTDDKMTLAFSRFYVNMFFPL
                     TDDLYFRVDIFKNGFWGHDQLGGSSSNNSSSSTPVGADPFNFGELHLHYVIFKNDRHD
                     LSLKIGRQFFEIGGEVYDYFLKDYLDAVTFNYASSRLGGFRIMAFDAFQLGADPTLHA
                     NYVRFFSHDNQRVRYFDGDTNVLRAGFVWDSRNLLNWKGPLTGDLLQNKIYAFGARYG
                     AVSRGGSDRSNLGASGNFADNDYNMMFGTRFLFNLPRKRYIIKTFADAAVSTGIDRRL
                     PTAANESQDVDTNGYAFGGGGSFELPRVIPSLDLDLNADGMYVSGSKYNENGIQTSHG
                     FTSFKGSRLGGIIMSRFYGVRPSSYTDSAGISDFPHDYERKAATAFAHLGVGTTLWQK
                     LRFGLDYWLAVDTGSSALYAQARANGTTSSITSVVLKAQDRLGKVLGHEVNFSADLAV
                     NRYWTLYFRAGVFLPGNFYSTPGVITDTPFGTDNAMGVQLGSKLIF"
     gene            complement(12639..13553)
                     /locus_tag="PLF85_02115"
     CDS             complement(12639..13553)
                     /locus_tag="PLF85_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNE18479.1"
                     /translation="MPLSDRSYSMSFHTRGADFRAVFVSAAEAINAATAQVHVDEKAF
                     YHFSEMVVCANLLAGQFRDVQDITLDLSTPKYLRNLVAEATPEGIFRATCQTSEELAS
                     VVDPAEIDEDVGSGLLHVSKRYARIPEPTRSTVGSRSKAIPNLVQQYLHESEQIATLM
                     LTGMKLRQGTNGNIEADKCFGLLIEALPEVSESKKFILTDNLERLESLTKYFGGAAAD
                     AADSKDVMDLLDDIYPGEQWIETSEVALVYRCRCSRQGYLDRLAHMARRDIGEIFGEQ
                     KEIEVRCGYCKNVFNYTRAEIEEHMRQL"
     gene            complement(13557..14531)
                     /locus_tag="PLF85_02120"
     CDS             complement(13557..14531)
                     /locus_tag="PLF85_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNE18480.1"
                     /translation="MRRSDVCSALWRDAFGSLLSATSVIVAGAGPSGLHLASALARRG
                     LRVTVYEPARCGGVRIPLLHACNLPKRGSPLWQAASAFSRTWYADATLLPAVERHVGP
                     FGEYFSIHTRTYLQLLRRRALAVGVQFVRAPLPAETDSTVFIATGAAAQPAAPAGWSA
                     ALAPLPGWESYFAMPAQAQPVAEELARRDIRTNYFIHRDRAGFIHLNGTERTAAQAFA
                     TALHPGQRHALFHGTRLTTRDRLPIVGFSLPNEMTTFNDLRAAVVQGRLKDLLRQQPG
                     VFFFTGMGYHAMTYSPFLADRAAKWLTGQAAEDEILLGALTPARFLPR"
     gene            complement(14518..15180)
                     /gene="mnmD"
                     /locus_tag="PLF85_02125"
     CDS             complement(14518..15180)
                     /gene="mnmD"
                     /locus_tag="PLF85_02125"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNE18481.1"
                     /translation="MKVFFSEKYDDIYFNNDNPEAEKDFVFIQGNNLPLRLQHNADLV
                     VAELGFGYGLNCALLLTAAREASFSGRLHFYSVEEKLPDFDAVSELLPKLTRSRQAYE
                     ALWQHRDTISRGETVTLFGAHVTVFPGQVNEFLASSGFLADAWFFDGFSPAKNPAMWS
                     DAVFASAFARTREHGTFATYSSAGWVRRNLVAAGFAVKKVTGFAGKREMLIGIKEKDA
                     PK"
     gene            complement(15191..16015)
                     /gene="speD"
                     /locus_tag="PLF85_02130"
     CDS             complement(15191..16015)
                     /gene="speD"
                     /locus_tag="PLF85_02130"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNE18482.1"
                     /translation="MKIKPLKKKLKLYGFNNLTKTLSFNMYDICYANSPRNQRAYIEY
                     IDEVYNAQRLTEILTNVAKIIGANILNIASQDYDPQGASVTMLVSENHLEVEKRNRFD
                     TDNEIPGPDSEAVVAHLDKSHITVHTYPESHPDQGISTFRADIDVSTCGQISPLKALN
                     YLLHSFSPDIVISDYRVRGFTRDVDGKKYFIDHKINSIQNFISDDIRQMYQMVDINIY
                     QENIFCTKMILKEFDLDNYLFGSAKKDFIPGEKKKIKQSLKREMNEIFYGRNIPKM"
     gene            16166..>16901
                     /locus_tag="PLF85_02135"
     CDS             16166..>16901
                     /locus_tag="PLF85_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18483.1"
                     /translation="MKKILAISLLAGFIGSLFAQAGTQPRESQPIVVEGGEQGPQTTP
                     VVRTITTKKAYAVTTSVPETVTTVQAPERVLVREGLLTWLSIGLRSSVDYHLTPKDGS
                     LSCLWFYGELYNTSWGIQAGLGYLWTPIATYSDAVGNTYNGNGTRGGLTLDLIGKYYW
                     WFARSWWIGAGFNYLALMGGQLRWFPTGTTGGTNPADGAAITNARWVDVVPGGGLFYA
                     QVGTGLKLAMGSGFNVVNFEPEFRALI"
BASE COUNT         3892 a         4584 c         4456 g         3769 t
ORIGIN      
        1 tgccctctga cgcatggtcg cggccatcaa taagtttgag cagggtcgat ttgcccgaac
       61 cgtttgcgcc cgtaatgcca tagcgcactc cttcggtgag atgcagcgag acacctgtga
      121 aaagttcacg cgaaccgaaa gacattccta aattttgcag gctaatcatt gtgttttatc
      181 tggcaatgcc ccaatcaggc gccacgaaat ttagagtcat agtccaggat cgaagcattg
      241 atcacattga gtgcctcatc gcgttcatag acagcgccga tctcgacttt ttgttttctg
      301 ccggggaaat ctttgtacgt gaggaaatag tggcgtatgc ggtcgatcag cgcctgcggc
      361 gcctctttca gcgaatgaat atttgcatag aggggatctt cagccattac agaaataatt
      421 ttgtcatcgg cctcttcgtc atcgatcagc cttaatcccc ctattggtct tgcacgcacc
      481 aggaagccgt taccggtaat ggagcgctcg gttaatacgc aaatatcgag cgggtcacga
      541 tcaccgcgca cgactttctt gtcacgcgcc agctggcgga cattctcatc gcaataagtc
      601 tgcggaataa aaccataaag cgtgggacag agcgatgagt acttctgcgg ccggtcaact
      661 ttcatcagac ccgaggcttt gtcgatttcg tacttaatag tatcggaggg tacgatttca
      721 atgaaggcag tcaccacagc gggcgcttca ctcccccgtg caactccgtg ccagggatgt
      781 gcggtaaagg gtgaagcttg cataaggcag tatcagcctt cgagacaggg ggtaaagaag
      841 agagcgggtg ctgtcggtcg agccaggaaa cttttttagt acctactaaa aaagtgcgaa
      901 acagtgagaa aatctcacca ttttgtggta tttaatagat aggatgcaga acatttcccc
      961 actcgaatac cactccactt cgatatccct tcgaccagcc tccaagcgac ggctaacagc
     1021 tgcccaaaaa atactgctcc gcaacggcgt tgtctggtcc gaatcagaat tgctgcgccg
     1081 cctggcgaag gtatacttgc gacactggag gggaacccag ctcaaatcag cgaccgcgag
     1141 acgctacaac cggtctatca agggcgaaaa gtacgttcgg gttgcctggt atgcagaccg
     1201 tctgctgtac tctattctgt ggcaaagggc aattcattcc ggcgaatcga tttcccgcat
     1261 gctggacttt tcgatacgca tgttcttacc ccgccttctg gaggagtact tgagaagccc
     1321 gatgcccgga tttgacaggt cgagtaaaaa ttcgatccac tggcagagac gattcgaacg
     1381 gcgttcaaac ccccaacccg agatctttat tacctaccaa tgcgaaactg aaatgaatac
     1441 ccttgccggc ctcaggtacc ggcaggagta caagatactc cgaaaaaatc agctctttac
     1501 ccacgacatc ccgtcacaaa ccagagacgc accgtgaata aacttgaccg cctgtttttt
     1561 ttcctgaacc tatcacatgg cgcgcagtag ttcatccaag tcatcgtcca aaggttacca
     1621 ggacggtttt tccgcttcag agattttcga aaaagaccag ggcatcacct acaaggactt
     1681 cctgatcctg cccgggtata ttgattttgc cccacaggac gttaatctcg aaactatggt
     1741 ttcacgcaat gtgaaaacca agatccccct catcagttcc ccgatggata cggtgaccga
     1801 aagcaagatg gccatcagcc ttgcgctgct gggcggcatc ggcattattc acaataacct
     1861 ttcgattgaa gaccaggctg cacaggtcga gcgggtcaag cgttacgaaa acggctttat
     1921 caccgacccg attgtactct ccccgaagca cacgatcgct gacatcgaca acattgcgga
     1981 acgttatggt ttcagcggcg tgcctatcac tgaagacggc acactccgca caaaactcgt
     2041 tggcattgtc gcgaatcgcg atgtggattt tgaacgtgac cgcagtattc cattggcgaa
     2101 ggttatgacc accgaacttg ttaccgcaac taagggcgta tcgcttttgc aggccaacga
     2161 gatcctccgc aaatcaaaaa agggcaagct gcctatcatt gacaaagaag gtcgcctcgt
     2221 tgcgctcatg agccgcaccg acctggtgaa gaacaaagaa tacccgctcg cgaccaaaga
     2281 tgcgcagaag cgcctgcgag tcggcgctgc gacttcgacg cacccgcatg accgcgaacg
     2341 tatcgacatg ctgattgcgc gtggcgtcga cctgctcgtc atcgattcgg cacaaggtta
     2401 ttcaaaattt caaatcgacc tgttgaaaga actcaagaag aaataccccg aggtagatgt
     2461 catggcgggc aatgtcgtga cgcgcgaaca gggtgaagcg ctcatcaagg ctggcgccga
     2521 cgcgctgcgc atcggcatgg gccctggttc tatctgcatc acgcaggaca ccatggcctg
     2581 cggacgctca caggcaacag cagtttatta cactgcacag atggccgcaa aatacaaagt
     2641 acccgtcatc gccgacggtg gtatcgccaa catcggcgac atcgccaaag cactcgccgt
     2701 cggcggcagc gcagcgatgg tgggttcgct gctcgcgggc agcaaagaag cgccgggtga
     2761 atacttctac gaaaatggtg tgcgcctgaa aaaattccgc ggcatggctt cgattgaagc
     2821 gatggaaggt tcgggcggcg gagctaaacg ctacaacgtt gaagatcaga agatcaaggt
     2881 ggcgcagggt gtctccggcg ctgtcgtcga caagggctcc ctttttgaat ttataccata
     2941 cctaatgcag ggcgtgcgcc acgcactgca ggacatgggt tgccgcgata ttgcttcgct
     3001 gcacaaggct ctgcatacgc gcaaactgcg tttcgagccg cgctccctcg ctgcgcaggc
     3061 gcaaggcgga gttcacgggt tgtactcgta caagaaaccg atcattggag cagactaagg
     3121 aggtcatcag tgaaactcta cggcagcatt acctcaccgt tcgtgcgccg cgtgcgcttt
     3181 ctttgccacc agcttgggca aagcttcgag atggtcgatt cgcttaccga agaaggccag
     3241 aatgccatgc gcgacaaaaa ccctatctgg aaagtcccgt gcgttgaaat cgacgacctg
     3301 gtcatctggg acagccacac gatcatcgat tacctgacgg aaaaatacaa gggtaacccg
     3361 aagctccttc gcagccccga tggcaacgag tactggcgcg agcgcaacct cgtcaccgcg
     3421 gccgatggct gtgtcgaatc ggcgattaac gtcttctacc tgaaaaaaga cggcgttgcc
     3481 gtgctcgatg tcgcgtacct gaagaaacag atggcgcggg tcgaatcgat tctcacatgg
     3541 ctcaaatcgc agctttatgg acactatttc accgtcgaag agcacattgg catgtcggag
     3601 ctcaccctgt attgtatact cgactggctg cggttccgcg aaatgtaccc ggtcaacgaa
     3661 gacccggttc tgaagggttt cctcgaacac cacaacagca acccaggctt tgtagccacc
     3721 aaattgcccg ggtaaaacag gatgttacaa ggtcggctgc ccgcacggat gcggtctgcg
     3781 cagacgaccg gttagaaagg atgtctagag gtcggcagcg cacatggatg tgctctttgc
     3841 tgacgaccgg ttagacagga tgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnccggtt agacaggatg tcaggtttac gggaacaaat accgatattt attctgtaca
     4021 cagtcgtttc tgtttaaaaa cggctgctca gcctatttct ggagagttct atgattcgtt
     4081 tcgttgtgac cgccattgcc accctgctga tttccggtag cgtttttgct tctgccgacg
     4141 gccaccatga cccggtgcat gatctgctga tgcacatcgg gttgacgata cttgtcgcaa
     4201 ctattctcgc ttatgtaggt catgtagcga agcagccact aattcttgcc tacctggccg
     4261 ccggggctct gattggaccg aattttggcc tcgggttgat caaaaccgaa aaagacatct
     4321 ccacaatctc agagctgggt ctcgtgctgc tgcttttcat gatcgggatg gagattgatc
     4381 tgaagaaaat caaagcttcg ggtaagtcgc tcatcctgac tggtattaca caatttgcga
     4441 tctgtgcagg cctgggcatt ctctttttcc agttgatcgg ctttcaactg ggcggaggta
     4501 aatacgacgc tttctacctc gccgtgtgct gcggtatttc ttctacggcg atcgtcgtca
     4561 aactgctcta tggcaagttt gaactcgaca cacttgcggg ccggctgacg ctcggcgtac
     4621 ttgtcttcca ggatatctgg gcaatcgtcg tgctcggtgt acagcccaac ctcgcgaacc
     4681 ccgaagtact gcagattctg ctctcgtttg ctaaagccgg tgtactcgta gccatcagcg
     4741 tagccatgag caaatatgta ctgccagcac tgttcagccg tattgcgaaa attcccgaac
     4801 tcacgctgct catgtcggtg ggctggtgct ttgctgtctg cgcggcagca cttggttttg
     4861 gcctctcgct cgagatggga gcgctgattg cgggcgtttc tatttcgact tttccttata
     4921 acctcgacgt cgttgcgaag accatcaccc tgcgcgattt ttttatcacc atcttcttcg
     4981 tatcgcttgg tatgaagatc cctaacccgg ttacccatat caacctggtg ggcatcgccg
     5041 tgattgttgc tgcgtttgtc atcctgacgc gctttctttc tatttacccg gttctgttta
     5101 gtctcaagaa cggcaaccgc gtcagtatcc tgacttcact caacctttca aacgtcagcg
     5161 agttctctct tgtaatcgcg gcgctgggcc ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnntcaacc tttcaaacgt cagcgagttc tctcttgtaa tcgcggcgct
     5341 gggccttgcc tcaggccaca tttcctctga tattctctcg atcattatct tcacttttgt
     5401 cattacatcg atctttgcac cgtacatgat caaatacaac cagcagcttc agggcaagat
     5461 caccggtgtt ttcgaaatga ttggccttag ggacaaacag aagtttgtcg aacctaccga
     5521 acaagagctc aacaaagata ttgccttgct cggcttcttt aaggtcgcga gctcgttcgt
     5581 caccgatgca gtcgaagaca ccggcaagaa cagtctcaaa gaaaaaattg tcgtggtcga
     5641 ttttaaccag accgtgcact ctaaactcaa gagtctcggc atacctgccg tctatggcga
     5701 tatcgggcac atcgatacgc tgcaccacgt agggttgcac cacactaagg ttgccatctc
     5761 aactattcct gacacgatac tggtcggcac cagcaatacg aagctgatta agcaaatcga
     5821 agatattgcc ccgcatgcga aaatcatcgt gacagcggaa aatcccgaaa gcgcgctgaa
     5881 aatgtacgaa gccggtgccg actatgtctt tctgccgcgg cagctggctt cggcgcacct
     5941 gatgccgatt gtccgtgaca tgctggctac aaccgaagaa ggcaattcac cggctgctaa
     6001 atcgcgccgc aaaaaagccg aagcgaagct tgagcagttg cgcgaagacg cgatcagcga
     6061 actcagggaa cggcacgaga tcatcgcctg accttcatca agtttattcg ccgcgcctga
     6121 cgacaatata ccggggtagc tactcggtat gatcaggcag gacaatacct ataaacgcta
     6181 ttttgatgcc ctgttactgg gcttcacgat atttgccgca atcgaagtgc cactgcacct
     6241 ggcattgcag tatcccaaag cgggttggcg gcagatcatc ggtcttgtct acccggtcat
     6301 tttcactgta gacatcctcg tgactttttt cacgactatc acagtcgatg gagaagagat
     6361 aacttcgcgc aaggaaatcg cggtgcgata cctgcgctcc tggttcattg tggattttct
     6421 tgcggcgacg ccttttgacc tgctgtttgc agaaggctgg ttatcagaat tgagcaatgc
     6481 cgcgcgttcg ctgcgcctgt tttcaccgcg ctatatacgc attctgctga tggtacgcat
     6541 gctccgcatc taccacatac tccccttctt gcagagaaca agcaagaaag acctgctcaa
     6601 cccgggaatc gtcaggctta tcttcacggt ttttatcgtt ttggtcattg cacactggat
     6661 ttcgtgcggc tggatagcat tgggaaagat tgacgaaaag gccgatgcgg taacaaatta
     6721 catcagagcg ctctattgga cgattacgac catcacaaca atcggttatg gcgatatcac
     6781 gcccacttca aatatacaga cgctgtatac tatggtcgtc cagctgattg gtgccggcat
     6841 gtacggttat atcatcggta atttggcgag cttactggca aacagcgacc ttgcacgcac
     6901 gcagttccgt gccaaactgg agaaagtcca gacctttctg cagtaccggg atgtgccgca
     6961 ggacctgcag gataacatcc gtacctatta cgactacctc tggaataacc ggcgcggctt
     7021 tgatgaatcg gctgtgctgc aggatctgcc gcagtcgctc aagctgcagg tagcactgca
     7081 cctcaaccgc gacatcattg aaaaggtgcc catgttcaag ggagctcccg acgaactgat
     7141 acggcaaatt gtgctcaatc ttcggccttt attattcacc ccgggcgatt acattttccg
     7201 taaaggtgaa atgggcgacc agatgtattt cataagtcgc ggaaaagtag aagttgtttc
     7261 tgaagatggt aagacagtat atgccacgct gggcgacgga gcattctttg gcgagattgc
     7321 cctgctcttc agcagcgagc gtaccgccag cgtgcgcgcg gccgagtact gcgacctcta
     7381 cacactcgac aagtatacct tcgataatgt actttcaaaa tttcccgggg tcgcggagca
     7441 tgtaaagatc atggccgaag aacggcgcca gcgcaccatc gccgagcgca gcgacgccac
     7501 tgcagtttcg cctgaaaaag cagtcatcga aaatgttcag gtttcctacc aatctggtcg
     7561 tgtgctcgtg gactggcccg atgtacctgg cgccagcggc taccaggtaa cccgctggga
     7621 tgaccaggca gaacgctggg tatatatcag ccggtcactt cttttaagcg agtgtcacga
     7681 tgtactgcca ctgcgccggc agaccaacac ctaccgggtg cgtgccatca tcggcgatgc
     7741 gtttggaccc tggtctaagg ccgtgcacgt caacgcctag gttattgaat caggtttctt
     7801 ctgtactttt tgcctggcgt atctttttga cgaagtactt cacgagaaaa taaagaattc
     7861 cgagaccaat cagcgcaacg atcgcctgct cgacgttctt gataaccccg acagcattgt
     7921 tgatctggtc gccgaaaaaa taacacgagt agacaatcgc gggtaccgaa atcattgcag
     7981 cgagcccgtc gaagagcaaa aaaacccgga aaggaatacc cagcgcgccc gatgacagga
     8041 aaaccagcga ccgtacgccg ggcataaagc gcgcggaaaa tatgacttta tagccatgat
     8101 tttgcaggtt ctgcttcacg gactccagcc gctctgcgtg catgattttg gacatccagc
     8161 gccattcaaa aacacggtag ccaaaacgcc ggccaatctg gaacattgct ccatcaccga
     8221 gcataacacc cgcaaggcca acgccgatca tcacaaacac gttcgccttg ccgtaatagg
     8281 ccatcaatcc gccggcgatg agcgtaatat cttcgggtac cggcaggcca aaaccgcaga
     8341 gcaaaagaat accaaataca atgaggtacg tccacggctc gtgaatcgac tggatcgcgt
     8401 gcacaatacc gtcaatattg atcatgacac tgacgaccgg gtaatcaacg ggccgtgtca
     8461 atcaattgcg ccccggcaga gatcgccctt gttcttcggg cagaaaccgg ctcagccagc
     8521 taatcgcctc ctgttcatcg ttaaacagcc tgacaggcac gcgcgggcgg ttaaactgga
     8581 taaaaatgtt accgaccatt ttctgaaacc gggaatgcac gagaaaacca aaggcattca
     8641 cgaatgaact gcggtccctg gcggcaaagt aggcgcgcgc ttcagaactg atggattgca
     8701 ggtgcctcag gttgatgagc agaggccgta accggccgcg gcaaagctcg ttcaccacaa
     8761 ggctgttctc gacggcatgg cggagttcta tgtcaatacc cggcttggcc tcagtgcgta
     8821 ttatgccttc aggcgtgcgc caggtagtaa atgcctctag ttcatgtctc tgcaccggct
     8881 aaggaaaata tgcgaatcgg agacatgccg gacagaactt tcaaaacgcg aaataaagct
     8941 tgccggcgcg cacggccggg tgcagcctat accactatga aaacaatttc aaaacttttg
     9001 ctcagcgtct ttctggcgac ctttgcgctt tccgcagaaa agcccgaggg caaaccaggt
     9061 cgcaagggcc ccgcaggaat gcccggccgt atgctcgaga tgatggatac caataaagat
     9121 ggcaaggtgt ctaaagaaga atggcaggcg caccatgacc agcgcttcac agaaatggac
     9181 accaacaaag acggtgctat cacgtcggca gaagtcaaag aacaccatgg caagatgcgt
     9241 gaaaaaaacg cagaaatgtg ccccgactgc caacgcggaa aaaaaggcaa gcacaaaggc
     9301 caccataaaa aaccagccga cgacgataac gactagccct gagaattatg gatgcagcgc
     9361 ttccgcagga taacccgggc gccatggagc gcctgctcga ttctgcgtat ggccaggtac
     9421 tgaatggctt cggcgtattt acgcccgccg accgcctggc gcaggaatac ctggcacaga
     9481 atgcgggcga tgcgcggcgc gctgcagccg acctgattgg ctggcagatg accaaagcgg
     9541 ccgcatcggg tttcgtcacg aaccttggcg gcataggcac attgcctgtg gcgattcccg
     9601 ccaatgtctc aagcgttatt tacctgcagc tgcgcatggt agccgcgatc gcgatcatgg
     9661 ccggttacga tgtaaggtct gacaaggtgc gcacgcttat ctatgtcacg ttgctcggct
     9721 cggctgccgc cgaggtactg aaagaagcag gcattaagct gggccagcga atcactacag
     9781 ccgcaatcat gcggctgacg ggccaggtag cgcgccgcat gaccagcagc gctgcggcac
     9841 ggctcatggc gcgcctgggc ctggcacagg ccggtaacct cgcgcggctt gtacctgtgg
     9901 cgggcggctt aattgccggc gccaccgatg cagcagccac gaaagcaatc gggctagtcg
     9961 ccacacaggt attccttcct gtaacagaag aaaatcccac tgggtcagat gttacacctg
    10021 gcaactaccc gcccgagcaa ataggttgac gctcccctcg gttacagtga ggtgagccca
    10081 tgaaagtcta catcgcggaa atcatcggca cggcgacgct tgtgctcttc ggctgtggta
    10141 gcgcggtttt tgccggcgcc tctatcggca tcgtgggtat ctcattttct tttggcctcg
    10201 cgctgctggc gatggcgtat gccattggcc cgatcagcgg ctgccatatt aacccggcag
    10261 tgagcgtcgg tgcttacatc gcgggtcgtc tgagcggtcg caggctcgta ggctacgtca
    10321 tcgcacaatc tttgggcgcc atcgtcggtg ctgcgctggt gtatgcaatt gccctgggtg
    10381 atcctgctta caaactcgcg gtgaatggtc tcggccagaa cggctttggc ccgggctacc
    10441 aggggggatt taacctcgaa tcggcgtttt tattcgaagc gatcgcgaca ttcatcttcc
    10501 ttgttgtcat tctcggttca acgagcagcg gcgcagcaaa gaagtttgcc gggattgcga
    10561 tcggtctttc gcttgtgctg attcacctgg tcggcattcg catcacaggc gtttctgtga
    10621 acccggcgcg tagcctcggg cctgcgctct ttgttggtgg gcaggcaatg gcgcagctgt
    10681 gggtttttat cgtggcacct ttactgggcg cagcattcgc cgggctgatg taccgcggcc
    10741 gcgtactcga agactgagcg tattcaccgt gtcttttttt cgtgcctgct gcgcggctgc
    10801 gctgctttcg gcgatcgccg caatttacgc gcagaagctg gatcccgaag ctgcgaataa
    10861 gcctgccgaa aatgcagaaa aagtaaaact cgccaagcct gaagacgtca cgactgacga
    10921 aaaaggttcc gaggttgcta cctccacggc ccgcgaagcg ctgctgccgg cccgcaaact
    10981 caaaagcgat gtcgacaaag aagtcgacga ccttccgttc ggcgtgccgc agatcgacaa
    11041 acgcgaaaac gtctttggcg acaaacagga caaagacgcc attctcaagc gcatggaata
    11101 ccagatcggc tttatcgcag aaggtgcggg cttcaacaat accgacctgc gccggctgga
    11161 tgagtcaacg gttgctacca tacagaacac cgacgacaaa atgaccctgg ccttttcgcg
    11221 tttctacgtg aacatgtttt ttccgctcac cgacgacctg tacttccgcg tcgatatttt
    11281 taagaacggc ttttgggggc atgaccagct cggcggttca agctcgaaca acagctcgtc
    11341 gtctaccccc gttggcgccg acccattcaa cttcggtgaa ttgcacctgc actacgtcat
    11401 cttcaagaac gaccggcacg acctgtcgct gaaaattggc cgccagtttt tcgagatcgg
    11461 tggtgaagta tacgactact ttctgaagga ctacctcgac gcagtcacct tcaactacgc
    11521 aagctcgcga ttgggcggat tccgcatcat ggcattcgac gctttccagc tgggcgccga
    11581 cccgacgctg cacgcaaact acgtgcgatt cttttctcac gacaaccagc gcgtgcgcta
    11641 tttcgacggc gatacaaacg ttctgcgcgc aggtttcgtc tgggattctc gcaaccttct
    11701 gaactggaaa ggcccgctga cgggcgacct gctacagaac aagatctatg cctttggcgc
    11761 gcgctacggt gcagtcagcc gcggcggctc agaccgctct aacctgggag catcgggcaa
    11821 cttcgccgac aacgactaca acatgatgtt tggcacgcgc tttctcttta acctgccgcg
    11881 taagcgctat attatcaaaa cttttgccga cgcggctgta tcgaccggta tcgaccggcg
    11941 tctgccgacg gcggcgaatg aaagccagga cgtcgacacc aacggctatg cgttcggcgg
    12001 cggcggcagc tttgaattgc cgcgggtgat cccctcgctc gatctcgacc tgaatgcaga
    12061 cggtatgtat gtttcgggca gcaagtacaa cgagaatggc atacagacct ctcacgggtt
    12121 tacctcgttc aagggcagcc gccttggtgg catcatcatg agtcgcttct acggtgtcag
    12181 gccttcaagt tacacggact ctgctggcat ttcggatttt ccgcacgatt acgaacgcaa
    12241 ggcggcaacg gccttcgccc acctgggcgt tggcacgacg ctctggcaaa aactgcggtt
    12301 cggtctcgac tactggctcg ccgtcgatac gggttcaagt gctctctacg cacaggcgcg
    12361 cgctaacggc acaacgtctt ctatcaccag cgtcgtcctg aaagcgcagg accggctcgg
    12421 caaggtgttg ggacacgagg tgaatttcag tgcagacctt gcggtcaacc gctactggac
    12481 cctgtacttc agggccggtg tgtttctgcc gggtaacttc tactcgaccc cgggcgtcat
    12541 caccgacacc cccttcggca cagacaatgc aatgggagtt caactcggtt cgaagctaat
    12601 cttctaaggg ctttgatttt tccgcggcgc ggcaaaaatc aaagttgccg catgtgctct
    12661 tcgatttcgg cccgggtgta gttgaaaaca ttcttgcagt aaccgcagcg cacttcgatt
    12721 tctttctgct cgccaaatat ttcgccgata tcgcggcgcg ccatgtgcgc cagccggtcg
    12781 aggtatccct ggcgactgca gcggcaccgg tagacaagag cgacttcaga agtctcaatc
    12841 cactgctcac cgggataaat gtcgtcgagg agatccatca catccttgct gtcggccgcg
    12901 tctgcagccg cgccgccgaa gtacttcgtc agcgattcga gtcgctcgag gttgtcggtc
    12961 aggataaact tcttcgactc ggagacctct ggtaacgctt cgatcagcag gccaaaacat
    13021 ttatctgcct cgatattgcc gtttgtaccc tgccgcagct tcatgcccgt gagcatgaga
    13081 gtcgcgatct gttcgctttc gtgcaggtac tgttgtacca ggttcggaat cgccttgctg
    13141 cggctaccta ccgtactgcg cgtcggctct ggaattcgcg cgtaacgttt actgacgtgc
    13201 agcaggccgc tgccgacatc ttcgtcaatc tcagcggggt ctaccaccga cgcgagttct
    13261 tcgctggtct ggcaggtcgc ccggaaaatt ccttcagggg tagcttcggc aacgaggttg
    13321 cgcaggtact ttggagtaga gaggtcgagc gttatatcct gcacgtcgcg gaactggccc
    13381 gccagcaggt tcgcacacac gaccatctcg ctaaaatggt agaaggcctt ttcgtcgacg
    13441 tgcacctgcg cggtcgccgc attgatggct tcggcagccg agacaaatac ggcgcgaaaa
    13501 tctgcacccc gggtgtgaaa ggacattgaa taagagcggt ctgaaagcgg catgtcttat
    13561 cgggggagaa accgcgccgg cgtcagtgcg ccaaggagaa tttcatcctc cgcggcctga
    13621 ccggtgagcc attttgctgc acggtcggcc agaaaaggcg aatatgtcat ggcatgatag
    13681 cccataccag taaagaagaa aacgccgggt tgctgccgca agaggtcttt taatcgtccc
    13741 tgcacgacgg ctgcacgtaa gtcgttaaag gtagtcattt cattaggaag agaaaaaccc
    13801 acgatcggca ggcggtcgcg cgttgtcagg cgtgtgccgt gaaagagcgc gtggcgttgc
    13861 cccggatgga gcgcagtggc aaaggcctga gccgcagtgc gctctgttcc attgagatgg
    13921 ataaaacccg cgcggtcacg gtgaatgaaa tagttagtgc gtatgtcgcg cctggccagt
    13981 tcttctgcaa cgggctgagc ctgcgcaggc atcgcgaaat acgattccca gccgggtagc
    14041 ggtgcaagag ctgcagacca gccagcgggg gcagctggct gcgccgcggc accggttgcg
    14101 ataaaaacgg tcgagtctgt ctctgcgggc agaggcgcac gcacgaattg aactcccaca
    14161 gcgagtgcgc ggcgccgcaa cagctgcagg taagtgcgcg tgtgaatcga aaaatattca
    14221 ccgaacggac ccacatgccg ctcgactgca ggaagcagcg tcgcatctgc ataccatgtg
    14281 cgcgaaaatg cgcttgctgc ctgccagagc ggtgaaccgc gcttgggcaa attgcaggca
    14341 tgcaacaacg gtatgcgcac accgccacag cgcgcgggtt catacacggt aaccctgagt
    14401 ccgcgccgcg cgagcgccga ggcaaggtgc aaaccactcg gccctgcacc ggcgacgatc
    14461 acagacgttg cagacagcag cgagccaaat gcgtcgcgcc agagtgcact gcatacatca
    14521 cttcggcgca tctttctctt ttataccgat cagcatctcg cgtttgccgg cgaagcccgt
    14581 aaccttcttt acagcaaaac ccgcagcgac aagattgcgt ctcacccaac cggcggagct
    14641 ataggtggcg aaggtaccat gttcgcgcgt gcgggcaaat gccgatgcaa aaaccgcatc
    14701 gctccacatg gcaggatttt ttgccggcga aaaaccgtcg aagaaccagg catcggcaag
    14761 aaacccggag ctcgcgagaa attcatttac ctgaccggga aaaactgtaa cgtgtgcgcc
    14821 gaaaagcgta acggtctcac cccggctgat cgtatcgcgg tgctgccaga gtgcttcata
    14881 tgcctgacgc gaacgcgtga gcttgggcaa cagctcgctg actgcgtcga agtcaggcaa
    14941 tttttcttca acggaataga aatgcaggcg accggaaaag ctggcctcgc gcgctgcggt
    15001 aagcagcaat gcgcaattca gcccgtaacc aaaaccaagc tccgccacta caaggtcagc
    15061 gttgtgctga aggcgcaagg gcaggttatt gccctggata aacacgaaat ccttttcggc
    15121 ttcgggatta tcgttgttaa agtagatatc gtcgtatttt tcggagaaga agaccttcac
    15181 agaacaggcg tcacatcttc ggtatgttgc gcccgtagaa aatctcgttc atctcgcgct
    15241 tgaggctctg cttgatcttc tttttttcgc cggggataaa atcttttttc gccgaaccga
    15301 acaggtagtt gtcgaggtcg aactctttga ggatcatctt ggtgcagaag atattctcct
    15361 ggtagatgtt gatatcaacc atctggtaca tctggcggat atcatcggag ataaagttct
    15421 ggatcgagtt gatcttgtga tcaatgaagt acttcttacc atcgacgtcg cgcgtgaagc
    15481 cgcggacgcg gtagtcgctg ataacgatat cagggctgaa agagtgcagc aggtaattca
    15541 aagccttaag aggagaaatc tgcccgcagg tcgagacgtc gatgtcggca cggaaggtac
    15601 tgataccctg gtcagggtgg ctttctgggt aagtatggac agtgatatgg cttttgtcga
    15661 ggtgcgcgac cacggcctca gaatcgggac ctggtatttc gttatcggta tcgaaacggt
    15721 tacgcttttc tacctcgaga tggttctcag agacgagcat cgttaccgag gcgccctgcg
    15781 ggtcgtagtc ctgcgaagcg atattgagga tgtttgcccc gataatcttg gccacgttcg
    15841 tgagaatttc ggtcaggcgc tgcgcattgt acacttcatc gatatattca atgtatgccc
    15901 gctggtttct ggggctgtta gcgtagcaga tgtcgtacat gttaaagctg agtgtcttcg
    15961 tgaggttgtt aaaaccatag agcttcagct ttttcttcag cggctttatt ttcatttcgg
    16021 gttcttgtcg cgttgggtca gaaaaacagg gggtcaaacc tatttagggc caaaaagatt
    16081 gacgtcctgt acaaaaagtc cgatataaag ccaagacggg gtcaaaggcc ctgcattttg
    16141 cgcaaaataa cttaagaggt attgaatgaa aaaaattctg gcaatctctc ttctggcagg
    16201 ttttatcggt tcactgtttg cacaggccgg tacgcagcca agagaatcac aacctatcgt
    16261 cgtagaaggt ggcgaacaag gtcctcagac cactccggtc gtccgtacca ttaccaccaa
    16321 gaaggcatat gccgtaacaa cttcggttcc cgaaactgtt accacagttc aggcacctga
    16381 gcgcgtgctc gttcgcgaag gcctgctcac atggctcagc attggtctca gaagctctgt
    16441 tgactatcac ctgactccga aagacggctc actgagctgc ctctggttct atggcgagct
    16501 ttacaacact tcatggggta ttcaggcagg tctcggttac ctctggacac ctattgctac
    16561 ctattcagat gcagtcggaa acacatacaa cggtaacggc acgcgcggtg gcctgacact
    16621 cgacctgatt ggcaagtact actggtggtt cgcccgctca tggtggatcg gtgctggttt
    16681 caactatctc gctctcatgg gtggccagct gcgctggttt cctacaggta caaccggcgg
    16741 tacaaaccct gctgatggtg ccgcgatcac caacgcacgt tgggtagatg tagttcctgg
    16801 cggcggcctc ttctacgctc aggttggcac gggcctcaag cttgctatgg gcagcggctt
    16861 caacgtggtc aacttcgaac cggaattccg cgcgctcatt c
//
LOCUS       DAOQJF010000021        16520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1676_length_16520_cov_5.297905, whole genome shotgun sequence.
ACCESSION   DAOQJF010000021 DAOQJF010000000
VERSION     DAOQJF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1676_length_16520_cov_5.297905"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLF85_02140"
     CDS             complement(<1..917)
                     /locus_tag="PLF85_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE18484.1"
                     /translation="MKKIHPLLVAAWAALFLSAGAVRADSFDAEQGFRSAMTAFKERN
                     FYSARLLLQEIVLKDGRGEYGDDAQYYLGMTYFYEGDYRTAQFEFKALQRDFPDSPFV
                     VRAAFWNGEAWFYRKQYREALEAHSAFVRKHRENALSASALYTIGFIYNEQKRYDEAV
                     QEFNRALRDYPDTPVAPALTLQLGIARFNAQEYSEARRAFETLLVKYTQADNLDAARF
                     WLGKSYFAENKFAEALREFSAVRNDFPASEHAAEAVYLAALCRYRQGNLAEAGELLSK
                     AAADFPQSSIYPFVRLRQAQLASEQNDETA"
     gene            complement(917..2284)
                     /gene="hslU"
                     /locus_tag="PLF85_02145"
     CDS             complement(917..2284)
                     /gene="hslU"
                     /locus_tag="PLF85_02145"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNE18485.1"
                     /translation="MLSDRKPADIVRYLDEFIIGQTEAKKAVAVALRNRERRLSLGEH
                     PLREEIHPKNIIMIGPTGVGKTEIARRLARLANAPFIKVEATKYTEVGYVGRDVESMI
                     RDLLMSAIKIVRSEHEERYKSEAEKRTEERLLDLMLSGKSETTAVGFQSAESSSTADE
                     DEKIARDIFRKKLRAGQLEEREVELKLSQQPPQVAVQMMAVPGMEELENQMQNILGDI
                     FPKKKEKRKITVGQARKLIYQEELDGLIDFEKIKEEAISRTENTGIVFIDEIDKIASR
                     GSKGSPDVSREGVQRDILPLVEGSTVNTRHGSVKTDHILFIAAGAFHIASVTDLIPEL
                     QGRFPIRVELKPLVKEDYISILKTPKNALAKQYQALLSTENVNVVFESSGYEAIAEIA
                     FEMNARNENIGARRLHTILERLFERVSFEPEKFTGGDVIVNRAFVEQELSEIVKNQDL
                     SRFIL"
     gene            complement(2286..3068)
                     /locus_tag="PLF85_02150"
     CDS             complement(2286..3068)
                     /locus_tag="PLF85_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE18486.1"
                     /translation="MHFKLSDLGGSKKLTRVVDIQTNEQNSLTRASLKELTAIMDETL
                     KDEKYASLLFTSTNPKFFSNGLDASNVMSTPDDKLAEEVGEIVLFFAHMLRFDKPVIT
                     EVTGYAMGGGAVITVGSDYKYMLEGKARISFSEVLVGLPLPGSFIDKIAKTVYPPFVN
                     SVCAGENYKAAEAKTIGLIDETAADTEGLRKLTVKKLDYLNRIPLSALQRTKQAINAP
                     ILERFETTLKHTYESFTTPIIVSNFRESMKALAEKRRPQYLD"
     gene            complement(3114..3725)
                     /locus_tag="PLF85_02155"
     CDS             complement(3114..3725)
                     /locus_tag="PLF85_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNE18487.1"
                     /translation="MKKPSLRVSSGKFKRAHIPLAKQHRQHQNSTTARVKEAAFQLIR
                     NRVDVSGEWIFYDLFAGSGQMGIEALSLGAAHATFVDIVPDRLGEIQRALAALEVPRD
                     TFTLVRAKAVKVLPEAFAQGDLPCVIWADPPYTYGGSPSSDPAILIALYRTALAENPQ
                     VAQRPLLVVQVHEKNPALAPEYLAANPDLEVYRYGSNCLLLLA"
     gene            complement(3722..5191)
                     /locus_tag="PLF85_02160"
     CDS             complement(3722..5191)
                     /locus_tag="PLF85_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE18488.1"
                     /translation="MSRVLLITKNTQLADYCRRLVPEGFSLNHTGKLNLRKETTQGIL
                     LFDSTLLAESSDTIVAAAAEQLGAAHGAVVIGLLVNEENRAQAFSLFPENLRHVQAFL
                     PYRIVAGKIQGFGAAELRWFLEVQQRTLRLTGEKVLTQNENRLLARRLARFETEAPSS
                     HGYPAFMQGRSQAIVRFREQVLHAAAHLPFVFVPGRDEAPADDFLEYYCALIRPQKRA
                     TYEVIDLEKTPSSLHMQAIWPQKKKQKQRVESAPVVCIRNLQRLGWANQAQLLQSLRT
                     AQAEKDGDRRRFVFIATNEIGQLVRRGTFRQELYSLLRKGIAELPPLYERPEDITHIA
                     AEYISQRKYTPLIDEKSAIAARILEKFDISAGYRGLFMTLDLLRDLQKSKGIPVFELL
                     GKSEKSEAFAAARSFLREEIEPSAESLFQNLAGGEKDALSLELVERHYIAAVCERYAW
                     QVTDAARHLGISRKTLYDKMRRYKLARPEKGGSGSQRAS"
     gene            complement(5417..5998)
                     /locus_tag="PLF85_02165"
     CDS             complement(5417..5998)
                     /locus_tag="PLF85_02165"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNE18489.1"
                     /translation="MRRRRKKSPGGQRSAPSRPRIALVGFRGVGKSAIARRLCEAWDM
                     PLLSLDEQIERQAGARIEDIVKSSGWQHFRDLEYAELARAATMEKVLLDCGGGIVEEA
                     DGSRSERKISLLRQNFFCVYISASEEKLLNRLKSLARNASRPALSATDSPESLMEIFR
                     RREPLYLELAHAVVDVSDTNVAESAFRLTQMFK"
     gene            complement(5998..6936)
                     /locus_tag="PLF85_02170"
     CDS             complement(5998..6936)
                     /locus_tag="PLF85_02170"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNE18490.1"
                     /translation="MKIDAQTRIYGIIGNPLSHTFSPAMHNAGFAARGINAVYLAFPL
                     HNLTHLKYSMNQWSIQGLSVTIPYKISVRRLLDRIDPLALQIGSVNTIRWGKDGLLEG
                     YNTDGPGAIMALKKEKVTIKGKKVLVIGSGGSARSIGFALAQEKPAQIGIMARNEMMA
                     MQLARNLTLNGETSNIAVHTRGENKHPQKSLAGLKPQKGRKWTNQPLPGADELATYDL
                     IINTTPMGMKGSDSAALSPLAPHELHKHQTVFDIVYNPAHTPLLRHAKKRGCGIVLGY
                     KMLLYQGVLQFELFTGKSAPVKEMEKALVTQIRKVF"
     gene            complement(6933..7316)
                     /locus_tag="PLF85_02175"
     CDS             complement(6933..7316)
                     /locus_tag="PLF85_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18491.1"
                     /translation="MLVEVRRELVRQDHRYLQKRFLQDAHNRIAIYIWQLPDDDEGEM
                     LRFQVAYRDIALDWAAERGMRLASVDDGDNPMGMKRSPVLHAENQVRKQSVERLCDYL
                     TEHPEKPSLLFIRDVIKSEILTGPQ"
     gene            complement(7316..8533)
                     /locus_tag="PLF85_02180"
     CDS             complement(7316..8533)
                     /locus_tag="PLF85_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNE18492.1"
                     /translation="MEKKSLFTPLLLVVLGAVIGLIFSGALIYEHNGSKTQIGSTVCE
                     AGETSSCDKAKESAMGKIFGLPLALYGYIFYGGIAALTCFLLLSVNETATSLLFWGAI
                     AALAFDVFLLVYSLFLLGGVCRMCAITYIASLLITVGAYLFKKRSETMIKPESSSLAA
                     KGSFSVVLLLTIVSGLFFHVNAQANQGPEAEGKTAEERRLREQLNEEFYRQWKAGETL
                     GLDKPRSGSKGAANPVLTIIEFADPLCPHCKDMGIVLGEFVKKYPDKVRVIFRHYPLD
                     IQCNSAMKRAFHVGACDLARAMECGDAQGKFWPMHDAIFAQQDVFMRNPVTEKAVESL
                     AMTAGANTAQLLGCFKSQSTIAKVKSDIAAGNKIKVTGTPTTIVNDRRLPGVPLEFIP
                     GILEKILLEESKR"
     gene            8590..8970
                     /locus_tag="PLF85_02185"
     CDS             8590..8970
                     /locus_tag="PLF85_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18493.1"
                     /translation="MQAAKNCSVRGAQVFGISIDVPVAEFFPRTQVSSSGKFIDEKRH
                     EAEAREEMLRRALEEQLARNNAAEHALPIERLMDRLSSGEKLNPEEYALISAEIARIQ
                     SRNQDHLARLESLLDRRSGEEGAG"
     gene            8973..9443
                     /locus_tag="PLF85_02190"
     CDS             8973..9443
                     /locus_tag="PLF85_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18494.1"
                     /translation="MLRLPVTGRVAALLLLALAFALTADSAKTFEEKERIARDMKKIS
                     KQLNVRCEYCHTDAERGLKEGDYTLLTQEGEYAQETMFPISKDFRVECSYCHTGASEL
                     NAAGERSLKDMKFMRRYKRQHKKNLSCKSCHMPGNQGEEFRRLTKFGRKNPGIP"
     gene            complement(9440..11479)
                     /locus_tag="PLF85_02195"
     CDS             complement(9440..11479)
                     /locus_tag="PLF85_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNE18495.1"
                     /translation="MAYPLNDKQSACVHHGEGPLLVLAGAGSGKTGVIVNRITQLIKK
                     GIKPTAICAVTFTNKAAREMRERVRELAGKNPKGIFIGTFHSLGLMILRENHEAAGIP
                     SNFVIMGQDDQLTLAREVLVLNGVDTDIFSEKHLIKAVSDWKNSGEARDDFFSDDVWA
                     LRVPQKPIAEIVAQYCERLSGMNALDFDDLILIPENLLGANEEVRERYRARFSHFLAD
                     EFQDTNPLQFRFLGRLMREPFNLCAVGDDDQSIYSWRGADTRIMLEFKQRFPEAEIIA
                     LEQNYRSLQYILDIANHLIAHNTARHPKNLYSAVKTPGQAIVFEAEDGLGEAEYVADT
                     IVALKVRQQLDYSAFCVLYRTNFQSRSFEEVLRQRNIPYHVSGSYQFYDRSEIKDVLA
                     YLRFFANQADDRSLMRILNLPRRGVSETAVQRLAQYAQLNKKHLWEILSEIEMCEIDI
                     SAATLSGILEFKEFVAAHLHEVRRSGNLAKAAQNLIDALQLEREYIRQGLDQQKVTNK
                     LLNVRELMRSIEDFENGDRQIAMIGENAERNLYTYLQFVALLTADDKEKESEVPRVQL
                     MTIHQAKGLEFDTVFVTGLEDGILPHQRSLDNEEEGSGTQGVEEERRLFYVALTRAKK
                     RLHLTLSRTRKNRGLVTDCTPSRFLDELPGNLLQWESTIPQTADLADLEKMLLNI"
     gene            complement(11608..13782)
                     /locus_tag="PLF85_02200"
     CDS             complement(11608..13782)
                     /locus_tag="PLF85_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE18496.1"
                     /translation="MQEILRKNIPSGIVVFFVALPLCLGIALAQNAPLMSGLVSGIIG
                     GIVVGMLGASQVSVSGPAAGLTVVVIGAIAQLGSFPAFTLAVFLSGIIQMGFSLLRMG
                     VVSKFIPGPVIKGMLAAIGIIIVLKQIPHLFGYDSDFVGDLEFIQPDGRNTFTELYAM
                     LRYLNPTAVLISFISIAVIMLWDKKLAPKHKYLKNIPGSILAVAIAVLVNLLAAKIDW
                     LKNLAPNHLVQLPLNPSPADFTRHLAAMDFSLLWSKKELWTVALTIAVIGSIETLLSL
                     EAADKIDPQRRIANKNRELVAQGTGNMLSGLLGGLPITAVIVRTVTNVNSGGTHRVSA
                     IWHGMLLVIAVFFIPQLLNHIPLAALASILAIVGYKLSSFRIFMDVWKSGREQFIPFA
                     VTVAAVVLTDIMLGVGIGVLFSVGFILLQNYRKGFYSSLQNGERVIRLAEDISFIHRP
                     GLSEELRNIPDNSIVTISAENTHNINHDIAELIAEFRESAPLRGITVREKSMPYIAPT
                     ATEAHARSSVYRRLIHGNRVWVEENLSQDRYYFEKLSKGQAPQVLWIGCADSRVPPDA
                     ITKTRPGEIFIHRNVANLVVHTDLNMLSVLQYAVEVLGVKQVIVCGHYECGGVRAAMQ
                     NSDLGLANKWLRNIKEVYQIHVRELSAVADPKEREKLLVELNVMEQVRNLAKTSIIQK
                     AWKKRAVEIHGWVIDLSTGFIKELPVRLNGPEDLDPIFRYKL"
     gene            complement(13850..14134)
                     /locus_tag="PLF85_02205"
     CDS             complement(13850..14134)
                     /locus_tag="PLF85_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18497.1"
                     /translation="MSGIAKRFLFALSLLLVLAAFTGMAAAAMPEMAETVAEEAEQME
                     TEGEVLPDFTPLSPPKTPEPRTAELSARAFNTHDYFRTGIFPEILTPPPL"
     gene            14248..15681
                     /gene="gatB"
                     /locus_tag="PLF85_02210"
     CDS             14248..15681
                     /gene="gatB"
                     /locus_tag="PLF85_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNE18498.1"
                     /translation="MKFIPTIGLEVHCQLNTRSKLFSTAATSFGADANTEVQTVCLGL
                     PGALPVLNYEAVKKAVRAGLALEGTIHERSKFDRKNYFYPDLPKGYQISQFFEPYCTK
                     AKIDIEVEGKKKTIGITRIHIEEDAGKLMHSEDPAVHESYVDLNRAGTPLIEIVSEPD
                     IDNSDEAVAYLTELKKILEYIDVSDCNMEQGSLRVDANVSVRPEGTDKLGTRVEIKNL
                     NSFKSVKAAIEYEISRQSELIESGGKVVQETRLYNAARDTTQSMRSKEEAHDYRYFPD
                     PDLVPLILKKDDIENIRKDLPELAHQKRVRYVSEFGLPEYDAGVLTAERSTATYFEEV
                     IRAGAPAKKASNWVMVEMLAIVKEKNKALSELFPPANLAELIHLIDSGMISGKIAKEV
                     FAEMVASGKKPKAIVDEKGMSQISDEKAILEIVQAVMAEHPASVADFKAGKDRALKHL
                     QGEIMKKTRGKVNPQVANKLLAEELAK"
     gene            complement(15730..16191)
                     /locus_tag="PLF85_02215"
     CDS             complement(15730..16191)
                     /locus_tag="PLF85_02215"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNE18499.1"
                     /translation="MAQKKRIALVAHDNCKAELIDWVGLHWPVLAAHDLTSTGTTGTL
                     IEQLVSEKQGRAPGNLLRLKSGPLGGDQQLGALIAEEKIDAIFFFWDPMHAQPHDVDV
                     KALLRICVLYNVVTACNRATADFIIASPLFSGNYDRKVPDFSGYTGRLLAT"
     gene            16242..>16520
                     /locus_tag="PLF85_02220"
     CDS             16242..>16520
                     /locus_tag="PLF85_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18500.1"
                     /translation="MLWLVALMVVIFVIGHYGLPEQVNPSAYQEMPSAIKIMRGAEPF
                     RLQGKTDVGFLLVHGFEDSPYTMRNIGNLLHSEGHTVIAPLLPGHGTNI"
BASE COUNT         3583 a         4474 c         4726 g         3737 t
ORIGIN      
        1 gctgtttcgt cgttctgctc cgatgcgagc tgtgcctgcc gcagcctgac aaaaggatag
       61 atggagctct gcggaaaatc agcagccgct ttcgagagca gctccccggc ctctgccagg
      121 ttgccctggc ggtagcggca gagcgcagcg aggtagaccg cttcggccgc atgttctgat
      181 gcggggaaat cgttgcgtac cgcactgaac tccctcagcg cttcagcgaa cttgttttcg
      241 gcaaagtagc tcttgcccag ccagaagcgg gcagcgtcga gattgtcagc ctgcgtgtac
      301 ttgaccagca gcgtttcgaa tgcccgccgg gcttcgctgt attcctgcgc attaaagcgt
      361 gcaataccga gctgcagggt caacgccggg gcgaccggtg tatccgggta gtcgcgcagc
      421 gcgcggttga attcctgtac ggcttcgtcg tagcgtttct gctcgttgta gataaagcca
      481 atggtgtaga gtgcagacgc agacagtgca ttttccctgt gtttgcggac aaaggccgaa
      541 tgtgcttcaa gtgcctcgcg gtactgtttg cggtagaacc acgcctcgcc gttccagaag
      601 gccgcgcgca caacgaaggg cgagtctgga aaatctctct gcagcgcctt gaattcgaac
      661 tgtgcggtcc ggtagtcgcc ttcgtagaaa tacgtcatgc ccaggtagta ttgtgcatcg
      721 tcgccgtatt cgccccggcc gtctttgagc acgatttcct gcagcagcag gcgggcagaa
      781 tagaagtttc tttctttaaa agccgtcatg gccgagcgaa aaccctgctc ggcgtcgaaa
      841 ctgtcggcgc ggactgcacc tgcagagaga aacaatgcag cccaggcggc aaccagcagc
      901 ggatgaatct tcttcatcac aggataaagc gcgagagatc ctggtttttc acaatctcgc
      961 tgagttcctg ctcaacgaac gcgcggttga cgataacatc gccgcccgtg aatttctcgg
     1021 gttcaaacga aacgcgctcg aacaggcgct ccaggattgt gtgcaggcgc cgagcgccga
     1081 tgttttcgtt gcgggcgttc atttcaaaag cgatttctgc gatcgcttca tagccactgc
     1141 tttcaaacac cacgttgacg ttttccgtcg agagcaatgc ctggtactgc tttgccagcg
     1201 catttttcgg ggtcttgaga atactgatat agtcttcttt cacgagcggc ttcagctcaa
     1261 cgcgtatggg aaaacgtccc tgcaattcag gaatcaggtc ggttaccgag gcgatgtgga
     1321 acgccccggc ggcaatgaag agtatgtggt cggtcttaac agagccgtgg cgggtgttga
     1381 ccgtcgaacc ttcgaccagc ggtagtatgt cgcgctgcac gccctcgcgc gagacgtcgg
     1441 gcgaaccttt gctgccgcgg ctcgcgattt tgtcaatttc gtcgatgaag acgatgccgg
     1501 tattctcggt gcgtgagatg gcttcttctt tgattttctc aaagtcgatc aggccatcga
     1561 gttcttcctg gtagatgagc ttgcgagcct ggccgaccgt aatcttacgc ttttcttttt
     1621 tcttggggaa tatatcgccg aggatattct gcatctggtt ctcgagctct tccatgcccg
     1681 gtacggccat catctgcacg gcgacctgcg gcggctgttg cgagagtttg agctccacct
     1741 cgcgctcttc gagctgtccc gcgcgcagct tcttacggaa aatatcgcgc gcgattttct
     1801 catcttcgtc tgcagtggag gagctttcgg cggactggaa gcctaccgcc gtcgtttcgg
     1861 acttgcccga aagcatcagg tcgagcaggc gttcttcagt gcgtttttca gcttctgact
     1921 tataccgctc ttcatgctca gaccggacaa tcttgatggc cgacattagc aggtcgcgta
     1981 tcatgctctc gacatcgcgg ccgacataac ccacctcggt gtacttggtt gcttcgacct
     2041 tgatgaacgg ggcattcgcg aggcgcgcga ggcgccgggc aatttcggtt ttacccacgc
     2101 cggttggccc gatcatgata atatttttcg ggtggatttc ttcgcgtaac gggtgttcgc
     2161 cgagcgagag cctgcgctca cggtttcgca gcgctaccgc taccgccttc ttggcttcgg
     2221 tctgcccgat aataaattca tcgaggtagc ggacaatgtc cgccggcttt cggtcggaga
     2281 gcatcttagt caagatactg tggacggcgt ttttcggcga gtgctttcat ggactcgcga
     2341 aagttactga cgatgatcgg agtggtaaac gactcgtagg tatgtttgag cgtcgtctca
     2401 aaccgctcga gtatcggtgc attgatcgcc tgcttggtgc gctgcagcgc tgaaagcggg
     2461 atgcggttga gatagtcgag ttttttcaca gtgagcttgc gcagtccctc tgtatcagct
     2521 gctgtttcgt cgatcaaacc gatggttttt gcttcggctg ctttgtagtt ttcgcctgcg
     2581 caaacgctgt tcacaaaagg agggtagaca gtcttggcga tcttgtcgat gaatgagccg
     2641 ggcagtggca gcccgacgag tacttcactg aacgaaatgc gtgccttgcc ttcaagcata
     2701 tacttgtagt cgctacctac tgtgatgact gcgccgccgc ccatcgcgta accggtaacc
     2761 tcggtaatta cgggcttgtc aaagcgcagc atgtgcgcaa aaaacagcac aatttcaccg
     2821 acttcttcag cgagcttgtc gtcgggcgtc gacatcacgt tggaagcgtc gagaccattg
     2881 gaaaagaatt tggggttggt cgaagtgaag agcagcgagg cgtatttttc atccttgagc
     2941 gtctcatcca tgattgccgt cagctctttg agcgaggcgc gtgtcagcga gttttgttca
     3001 ttggtctgga tatctacaac acgggtcagc ttcttgctgc cgccgagatc agaaagtttg
     3061 aagtgcatag gcaaccaaac ctgaccctga gtcagcgggt aaagctgatt gggtcaggcc
     3121 agtaacaaca ggcaattcga accgtagcga tagacttcga gatcggggtt tgccgccagg
     3181 tattccggtg cgagcgcggg gttcttttcg tgtacctgta cgaccagcag cggtcgttgt
     3241 gcaacctgcg gattttctgc cagcgcggta cggtaaagcg caataagaat tgccggatcg
     3301 ctcgacggtg agccaccgta ggtatagggc gggtcggccc agatgacaca gggcagatca
     3361 ccctgggcaa aagcttccgg cagaaccttc acggcttttg cccggacgag ggtaaaagta
     3421 tcgcgcggta cttcaagcgc tgcaagcgca cgctgaatct cgccgaggcg atcgggcacg
     3481 atgtcgacaa acgtggcgtg ggcggcgcca aggctcagcg cttcgatacc catctggccg
     3541 ctgccggcaa aaagatcata aaaaatccat tcgcccgaaa catctacacg attgcggatg
     3601 agctggaatg cggcttcttt aacgcgtgcc gtcgtggagt tctggtgctg gcggtgttgt
     3661 ttcgcaaggg ggatgtgcgc gcgtttgaac ttgcccgaag agaccctgag cgacggtttt
     3721 ttcacgaggc cctttggctg cctgatccgc ctttttccgg ccgcgcaagt ttgtagcggc
     3781 gcatcttgtc atagagcgtc ttgcgcgaaa ttcccaggtg tcgggcagcg tcggtcacct
     3841 gccaggcgta gcgttcgcag actgcggcaa tataatggcg ttcgaccagt tcgagcgaaa
     3901 gggcatcctt ttcaccgccg gcgagattct ggaacagcga ctcggcgctg ggttcaattt
     3961 cttcacgcaa aaagctgcgc gctgcggcaa aagcttcgga tttctcgctc ttgccgagca
     4021 gttcaaagac gggaataccc ttactcttct gcaggtcacg cagcaggtcg agagtcatga
     4081 acagaccgcg gtagccggcg gaaatatcaa acttctcgag aatgcgcgcc gcgatggcag
     4141 atttttcgtc gatcagcggt gtgtacttac gctggctgat atactcagcc gcgatgtggg
     4201 taatgtcttc agggcgctcg tagagcggcg gtagctcggc tattcccttg cggagaaggg
     4261 agtagagctc ctgcctgaaa gtgccccgcc tgaccagttg gccaatctca tttgtcgcaa
     4321 taaagacgaa acggcgccgg tcgccgtctt tttcagcctg cgcggtacgc agcgactgca
     4381 atagctgggc ctggtttgcc cagcccaggc gctgcaggtt gcggatgcag acgacgggtg
     4441 cagattctac gcgctgtttc tgtttcttct tctgcggcca gatcgcctgc atatgcagcg
     4501 acgaaggcgt tttttccagg tcgatgactt cgtaagtggc gcgcttctgc ggccggatca
     4561 gggcacaata gtactcaaga aaatcgtccg ccggagcctc gtccctgccc ggaacaaaaa
     4621 cgaatggcag atgggcagcg gcatgcagta cctgctccct gaagcgaacg atcgcctgcg
     4681 atcggccctg catgaatgcc gggtaaccat gtgaagaggg agcttcagtt tcaaagcgtg
     4741 ccagtcgccg tgcgagcagc cggttttcgt tctgcgtcag caccttttct cccgtcaggc
     4801 gcagcgtccg ttgctgcact tcgagaaacc agcggagttc agcagcgcca aacccctgga
     4861 tcttgccggc tacgatgcgg taaggcagga atgcctgcac gtgccgcagg ttttccggga
     4921 acagcgaaaa tgcctgcgcg cggttttctt cattcactag caggccaata accaccgcgc
     4981 cgtgggcggc acccagctgt tctgccgcgg ccgcaacgat ggtgtctgag ctctcggcca
     5041 gaagtgttga atcaaacagc aggatacctt gcgtggtctc tttgcgcagg ttgagtttcc
     5101 ccgtgtggtt cagtgaaaag ccttcgggca ccaggcgacg gcagtaatct gcgagctggg
     5161 tgtttttcgt gatgagcagc acgcgagaca tcgggctcga cccagttcag aatcgccata
     5221 gtgcgtcaac tcggagcaag gatgctcgag ctcgcacggc aacaggaggt tgctctttcg
     5281 ggcgagccgg agcaaggatg ctcgagctcg cacggcaaca ggaggttgct ctttcgggcg
     5341 agccggagca aggatgctcg agctcgcacg ggcgacaggc ctttctttta aatgattttg
     5401 cggctttgcc gcaaaatcat ttaaacatct gggtgagacg aaacgcgctt tcagcgacgt
     5461 tcgtatccga tacatcgacc accgcgtgtg ccagctcaag gtagagcggt tcccggcggc
     5521 gaaaaatttc cataagagat tcgggagagt ctgtggccga cagcgcggga cgcgatgcat
     5581 tgcgcgcaag acttttcaag cggtttaata acttttcttc actggcagaa atatagacgc
     5641 agaaaaaatt ctgccgaagc agcgatattt tccgctcact gcggctgcca tccgcttctt
     5701 cgacgatgcc gccaccgcag tcgagtaaaa ctttttccat agttgcggcg cgcgcaagtt
     5761 ctgcgtactc aaggtcccga aagtgctgcc agccagatga tttgacgata tcttcgatgc
     5821 gcgcacctgc ctgccgctca atctgctcat cgagcgaaag cagcggcata tcccaggctt
     5881 cacacaggcg gcgcgcgata gccgacttgc ctacgcccct gaagccaacc agcgcaatgc
     5941 gcggtcttga aggcgcgcta cgctggccac cgggcgattt tttgcgtctg cgccgcatca
     6001 aaaaaccttc ctgatctgcg tgacgagggc tttttccatt tctttcaccg gtgcactttt
     6061 gccggtgaac agctcaaact gcaggacacc ctggtagagc agcatcttgt aaccgaggac
     6121 aatcccacaa ccccgctttt tcgcatgacg gagcagaggg gtatgcgcag gattatagac
     6181 aatatcaaat accgtctggt gcttgtgcag ctcatgcggg gccagtggac taagtgccgc
     6241 tgaatcagag cctttcatac ccatcggggt ggtattgata atgagatcgt acgtggcaag
     6301 ctcgtctgcg ccgggtagcg gctggttggt ccacttacgg cccttttgcg gtttgaggcc
     6361 cgcgagtgac ttctgcggat gtttattttc accccttgtg tgcaccgcga tgttgctggt
     6421 ctctccgttc agcgtaaggt tacgcgccag ctgcatggcc atcatttcat tgcgggccat
     6481 gatgccgatc tgcgcaggct tttcctgtgc cagcgcgaag ccgatactgc gcgccgaacc
     6541 gccagagcca atcacgagta ccttcttgcc cttgattgtg actttctctt tcttcagcgc
     6601 cataatcgcg ccggggccgt cggtattgta gccctcgagt aaaccgtctt ttccccagcg
     6661 gatagtattc acagaaccaa tctgcagcgc cagcggatcg atgcggtcca gcagccggcg
     6721 aacagagatc ttgtagggta tggtgaccga aaggccctgg atgctccact ggttcattga
     6781 atacttcagg tgcgtcaggt tgtgcagtgg gaaagccagg tagacggcgt tgataccgcg
     6841 cgcagcgaac ccggcgttgt gcatcgccgg tgagaacgta tggctcaacg ggttgccgat
     6901 gatgccgtag atgcgtgtct gcgcgtcgat cttcattgtg gtcctgtcag aatttccgat
     6961 ttaataacgt cgcgtataaa gagcagcgaa ggtttttccg ggtgttccgt caggtaatcg
     7021 cacaaccgct ccaccgattg ctttctgacc tggttttctg cgtgcaatac cggtgagcgt
     7081 ttcataccca tgggattgtc cccgtcatcg acagacgcga gccgcatgcc gcgctctgcc
     7141 gcccagtcga gcgcaatatc gcggtacgca acctggaagc gaagcatttc accttcatca
     7201 tcgtcgggca gctgccagat gtagatggca attctgttat gtgcatcctg taagaaacgc
     7261 ttttgcagat agcggtggtc ttgcctgacg agttcgcgcc tgacctctac cagcattacc
     7321 ttttggattc ttcaagcagt attttctcaa ggataccagg tatgaattca agcggtacac
     7381 ccggcaggcg ccggtcgtta acaattgtcg tcggtgtccc ggtgaccttg atcttgttgc
     7441 cggctgctat gtcagacttc acctttgcaa tagtagactg gctcttgaag cagccgagta
     7501 gctgcgcagt attcgcacca gctgtcatcg cgagtgattc aaccgctttc tcagttacag
     7561 gattgcgcat gaagacatcc tgctgtgcga agattgcatc gtgcatcggc cagaacttgc
     7621 cctgcgcgtc gccgcattcc atggcgcgcg ccagatcgca ggcaccgacg tggaaggcac
     7681 gtttcatcgc gctgttgcac tggatgtcga gcgggtagtg gcggaagatg acccggacct
     7741 tgtcaggata cttttttacg aattcgccca gcacaatgcc catatctttg cagtgcgggc
     7801 agagcggatc ggcaaattcg ataattgtca gtaccgggtt agcggcgcct ttgctgccgc
     7861 tgcgcggctt atccagcccc agcgtttcac cggctttcca ctggcggtag aattcttcgt
     7921 tcagctgctc gcgcagccgg cgttcttcgg ccgttttacc ttcggcctcg ggcccctggt
     7981 tcgcctgcgc gttgacgtgg aaaaacaatc ctgacacaat agtcagtaaa aggaccacgc
     8041 taaagctgcc cttggccgca aggctcgatg attctggttt gatcatagtt tcactccgct
     8101 ttttgaagag gtatgccccg acggtaataa gcagcgaggc gatataggtg atagcacaca
     8161 tacggcagac gccaccgaga aggaagaggg aatacacgag caggaaaaca tcaaaagcga
     8221 gtgcagctat cgctccccag aaaagcaggc ttgtcgctgt ttcgttcacg ctcagcagca
     8281 ggaaacaggt aagggcggcg atgccgccgt aaaagatata gccatagagt gcgagcggca
     8341 gaccaaaaat cttgcccata gccgattctt ttgccttgtc gcagctgctg gtttctccgg
     8401 cctcgcagac tgttgagcca atctgcgttt tggaaccgtt gtgctcataa atgagggcgc
     8461 ccgaaaaaat aaggccaatg acagcgccga gtacgacgag cagcaggggg gtaaacaggg
     8521 actttttttc catgagtaac ctttaacaaa accgatcgga gcataagcgg acaagcattt
     8581 ttgtcgcgta tgcaggctgc aaaaaactgt tctgtgcgtg gagcacaggt cttcggtatt
     8641 tcgattgacg tgcctgtcgc agaatttttc cctcgaacgc aggtgagttc atcgggcaaa
     8701 ttcatcgatg agaaacggca cgaggccgaa gcgcgtgaag aaatgctgcg ccgtgcgctc
     8761 gaagagcagc tcgcgcgcaa taatgccgcg gagcacgcgt tgcccattga gcgcctcatg
     8821 gatcgcctta gttcgggcga aaagctgaac cctgaagaat acgccctcat ctcggctgaa
     8881 atagcccgaa tccagtcgcg caaccaggac cacctcgcac ggctcgagag cctgcttgac
     8941 cggcgctcag gagaagaggg ggccggctga ccttgctacg cctgcctgtg acaggcagag
     9001 ttgccgcgct tctcctgctc gctcttgcct ttgccctgac cgcagacagc gcaaaaacct
     9061 ttgaagaaaa agagcgcatc gcgcgcgaca tgaaaaagat ctcgaagcag ctgaatgtcc
     9121 gctgtgaata ttgccatacc gatgcagagc gcgggcttaa agagggcgac tatacgctgc
     9181 tgacgcaaga gggcgaatac gcgcaggaaa ccatgtttcc gatcagcaag gatttccggg
     9241 tcgaatgttc gtactgccac accggggcat cagaactcaa tgccgccggc gagcgttcgc
     9301 tcaaggacat gaaattcatg cggcgctaca agcgtcagca caagaaaaac ctttcctgta
     9361 agagctgcca catgccgggt aaccagggtg aggagttcag gcgtttgacg aagttcggca
     9421 ggaaaaaccc aggaattcct tagatattca gcagcatctt ttcgaggtcg gcaagatcgg
     9481 cagtctgcgg gatggtactt tcccactgca acaggttgcc cggcagctcg tcgagaaacc
     9541 gcgaaggggt gcaatcagta acgaggccac ggttcttgcg tgtgcgcgag agcgtcaggt
     9601 gcaggcgttt cttcgcgcgg gtgagcgcca cgtagaacaa gcggcgctcc tcttcgacac
     9661 cctgggttcc agagccttct tcttcattgt cgagcgagcg ctggtgaggc agaatgccgt
     9721 cttcgagacc ggtaacgaac acggtatcaa actcgagtcc cttggcctgg tgtatggtca
     9781 tcagctgcac gcgcggcact tcgctctctt tctctttgtc gtctgcggtc agcaaggcca
     9841 caaactgcag gtaggtatag aggttacgtt ctgcattttc accgatcatc gcaatctgcc
     9901 ggtcgccgtt ttcaaaatct tcgatcgaac gcatgagctc gcgcacgttc agcagcttgt
     9961 tcgtcacctt ctgctggtcg agtccctgcc tgatatactc gcgttcgagc tgcagggcat
    10021 cgatgaggtt ttgtgctgcc ttcgctaggt tgccgctgcg ccggacttcg tgcaggtgcg
    10081 cggcgacgaa ttccttgaat tcaagaatac cagacagcgt cgcggcgctg atgtcaattt
    10141 cgcacatctc gatttccgag agtatttccc acaggtgctt tttgttcagc tgtgcgtatt
    10201 gcgcgagccg ctgcaccgcg gtctctgaaa ccccgcgccg tggcaggttc agtatgcgca
    10261 tcagcgaccg gtcatcggcc tggttagcaa aaaaacgcag gtaagcgaga acatctttga
    10321 tctcactgcg gtcgtagaac tggtagctgc cgctgacatg gtaggggatg ttacgctggc
    10381 gtaacacctc ttcgaatgaa cggctctgga agttggtgcg gtataggacg caaaatgcag
    10441 agtaatccag ctgctggcgc accttgagcg cgactatcgt gtcggctaca tactctgcct
    10501 cacccagacc gtcttcggcc tcaaagacga tcgcctgacc aggtgttttg accgcactgt
    10561 agagattttt cgggtggcga gcggtgttgt gcgcaatcag gtggtttgcg atgtcgagta
    10621 tgtactgcag cgaacggtaa ttctgctcga gcgcaataat ctctgcttcg ggaaaacgct
    10681 gcttgaattc gagcatgatg cgcgtgtcgg cgccgcgcca tgaatagatc gactggtcgt
    10741 cgtcgcccac tgcgcagaga ttgaatggct cgcgcatcag cctgccgaga aaacgaaatt
    10801 gcagcgggtt ggtgtcctgg aattcgtccg cgagaaagtg tgaaaaacgt gcgcggtagc
    10861 gctcccggac ttcttcgttg gcgccgagca ggttttccgg tatgagtatc aaatcgtcga
    10921 aatcgagcgc attcataccc gaaaggcgtt cgcagtactg cgctacaatc tctgcgattg
    10981 gcttctgcgg cacgcgcagg gcccagacgt catcggagaa aaaatcatcg cgcgcttcgc
    11041 ccgagttttt ccagtcagaa actgccttaa tcaggtgctt ttcgctgaag atgtcggtat
    11101 cgacgccgtt caggacgagt acctcgcgcg cgagcgtgag ctggtcatcc tgccccatga
    11161 tgacgaaatt cgaagggata cctgctgcct cgtggttttc acgcaggatc atgaggccca
    11221 ggctgtgaaa ggtaccgatg aaaattccct tggggttttt tcctgcaagt tcgcgcacgc
    11281 gttcgcgcat ttcccgcgct gccttgttgg tgaaggtcac ggcgcagatg gccgtgggtt
    11341 tgattccctt ctttatgagc tgcgtaatgc ggttaacgat gaccccggtc tttcccgagc
    11401 cggcccccgc caggacgagc agcgggccct cgccgtggtg gacgcaggcg gattgtttgt
    11461 cgttaagcgg gtaggccacc gtcggacaga ctgacaatga gacataatac ggatagtatc
    11521 tttgcagcca aaccgctcag cgaggccccg gattccccta agaccgcctg ccggttgtcg
    11581 ccggcgaggg ggttttttag gcaaacatca gagcttatag cggaagatag gatccaggtc
    11641 ttcaggaccg ttgaggcgca cgggcagctc cttgataaag ccggtcgaaa ggtcaatgac
    11701 ccagccgtga atttccacgg cgcgcttctt ccatgctttc tgtatgatcg atgtcttggc
    11761 caggttgcgt acctgctcca tgacattgag ctccaccagg agtttttcgc gctctttcgg
    11821 atctgcgaca gccgagagct cacgcacgtg tatctggtag acttctttga tgttgcgcag
    11881 ccacttgttg gcaagcccga ggtcgctgtt ctgcattgct gcgcggacgc cgccacattc
    11941 gtagtggccg cagacaataa cctgcttcac cccgagtact tcgacggcat actgcagaac
    12001 ggaaagcata ttgagatcgg tatgcacgac gaggttggcc acattccggt ggataaaaat
    12061 ttcgcctggc cgggttttgg taatcgcatc ggggggtaca cggctgtctg cacaaccaat
    12121 ccagagcacc tgtggcgctt gccccttcga aagtttttcg aagtagtagc ggtcctgcga
    12181 caggttttct tcgacccaga cccggttgcc gtggatcaac ctgcggtaca cggaagaccg
    12241 ggcgtgtgct tcagtcgcgg tcggggcgat gtagggcata cttttttcgc gaaccgtgat
    12301 gccgcggagg ggagcactct ctctgaattc agcaatcagt tctgctatgt catggttgat
    12361 attgtgcgtg ttttccgcgc tgatggtaac aatcgaatta tctggtatgt tacgcagctc
    12421 ttcactcaga cccggccggt ggataaaact gatatcttcg gccaggcgga tcacgcgctc
    12481 gccgttctgc agtgagctat agaagccctt gcggtaattc tgcaggagta tgaatccgac
    12541 agaaaataaa acaccgatac cgacgccgag catgatgtcg gtgagtacga ctgccgctac
    12601 tgtcactgca aaaggaatga attgctcgcg acccgatttc cagacgtcca tgaaaatgcg
    12661 gaagctgctg agtttgtagc ccacgattgc gagtatactc gcaagagccg ccagcggaat
    12721 gtggtttaac agctgcggta taaagaagac ggcaatcacc agcagcatac cgtgccagat
    12781 cgccgatacc cggtgggtcc cgcctgagtt cacgttggtg accgtgcgca cgatgactgc
    12841 cgtgatcggc agaccgccca aaaggccgct gagcatattg cctgtccctt gcgccaccag
    12901 ctcgcggttc ttgttggcga tgcggcgttg cgggtcgatt ttgtctgcgg cttcgagact
    12961 cagcagcgtt tcaatgctac cgatcactgc gatggtgagc gcaaccgtcc agagttcttt
    13021 tttgctccag agcaggctga agtccattgc agcaaggtgg cgggtaaagt cagctgggct
    13081 cgggttgagc ggcagctgca caaggtggtt gggagccagg tttttcagcc agtcgatttt
    13141 agctgcgagc aggttcacga ggactgctat tgcgaccgca agtattgaac ccggaatatt
    13201 tttcaggtac ttgtgcttcg gcgccagttt tttatcccag agcataatca ctgcaataga
    13261 aatgaatgat atgagtacgg cagtcgggtt gaggtacctc agcatggcat atagttccgt
    13321 aaaagtgtta cggccgtcgg gctgaataaa ttcgagatcg ccgacaaagt cggaatcata
    13381 gccgaacagg tgcggaatct gcttcagcac gatgataatc ccaattgccg ccagcatacc
    13441 tttaatgacc ggacctggta tgaatttgct gacaacgccc atgcgcagca gtgaaaaccc
    13501 catctgtatg ataccagaga gaaatacggc gagagtaaac gcgggaaacg aacccagctg
    13561 cgcgatggcg ccgatcacga cgacggttag ccccgcagcc gggccgctca cgctcacctg
    13621 tgaggctccc agcataccga cgacgatacc gccgataatg cctgagacga ggcccgacat
    13681 gagcggggcg ttctgcgcca gcgcaatgcc gaggcagagc ggtagtgcga caaagaagac
    13741 aactatccct gatggtatat tttttcgtaa aatttcctgc attttattct cctgagatcg
    13801 cataccttct gaagccgggc aggcaggatt tgcggactaa gccaggggct tatagcggag
    13861 gtggtgtcag aatttcagga aagattccgg ttcgaaagta atcgtgggta ttgaatgcac
    13921 gggctgaaag ttcagctgtt cgtggttcgg gggttttggg cggggacagc ggagtgaaat
    13981 ccggcagtac ctcgccctca gtctccattt gttcggcctc ttcagcgacg gtctcggcca
    14041 tttcaggcat cgccgcagcg gccatacccg tgaaggcggc aagaaccagc agaagtgaaa
    14101 gggcgaagag gaatcttttg gcgatgccag acacgcccgg tcaaggcaca cggcaaatct
    14161 gccctgacaa gcgcaacagc atattcaaac cggcgtgccg caataaaaac ccgttcgacg
    14221 aggtgtatcg ccggcaaaaa atgaacgatg aagttcattc cgaccatcgg tcttgaggta
    14281 cactgccagc tcaatacgcg gagcaaatta ttttcaacag cagcaacgtc gtttggcgcc
    14341 gacgcgaata ccgaagtaca gacagtctgc ctcggcttgc cgggtgcgct accggttctc
    14401 aactatgagg ccgtgaaaaa ggcagtgcgc gcgggccttg cgcttgaagg caccatacac
    14461 gagcgcagca agtttgaccg caagaactac ttttaccccg acctgccaaa gggttaccag
    14521 atatcgcagt ttttcgaacc ctattgtacc aaggcgaaaa tcgacataga agtcgagggc
    14581 aaaaagaaga ccatcggcat cacccgcatc cacattgaag aagatgcggg caagctcatg
    14641 cactccgaag acccggcagt gcacgaaagt tacgtcgacc tcaaccgagc gggtacccct
    14701 ctgattgaga tcgtttctga accggacatc gataactccg acgaggcggt tgcatacctc
    14761 accgagttaa agaaaattct cgagtacatc gatgtgtccg actgcaacat ggagcagggc
    14821 tcgctgcgcg tcgatgccaa cgtgtcggtg cgcccggagg gaacggacaa gctcggaacg
    14881 cgcgtcgaga tcaagaacct gaactccttc aaatcggtga aggctgccat cgagtacgaa
    14941 atcagccgcc agtcagaact catcgaatcg ggcggcaagg tggtgcagga aacacggctc
    15001 tacaacgctg cccgcgacac cacgcagtcg atgcgcagca aagaagaggc gcacgactac
    15061 cgctactttc cggaccccga cctcgtgccg ctgatcctga aaaaagacga tattgaaaat
    15121 atccgcaaag acctgcccga actggcgcac cagaagcgtg tgcgttatgt cagtgaattt
    15181 ggcctgcccg aatatgacgc gggtgtacta actgcagagc gttcgacggc gacgtatttc
    15241 gaagaagtca tcagggcagg ggcaccggcc aagaaggctt cgaactgggt catggtagaa
    15301 atgctcgcga tagtcaaaga aaagaacaaa gccctttcag agttatttcc gccggcaaac
    15361 ctcgccgaat tgattcacct cattgactcc gggatgatct caggtaagat cgccaaagag
    15421 gttttcgccg agatggttgc cagcggcaag aaaccaaaag caatcgtcga tgagaagggc
    15481 atgtcgcaga tcagcgatga aaaagcgatc ctcgaaattg tgcaggccgt catggcagaa
    15541 catccggcat cggtggctga cttcaaagcc ggtaaagacc gggcgctgaa acacctgcag
    15601 ggtgaaatca tgaaaaagac ccgcggcaag gtgaacccgc aggtggccaa caagctgctc
    15661 gccgaagaac tggcaaaata gccttctggc tcctgaaaag ggaacctgct aagcacttct
    15721 tcaagccacc taagtggcga gcagtcgacc tgtgtagccg gaaaaatcag gcaccttccg
    15781 gtcatagttg cctgagaaca gcggcgatgc tatgatgaaa tctgcggttg cgcggttgca
    15841 ggcagtgacg acgttataga gcacacagat gcgcagcagt gctttaacat ccacatcgtg
    15901 tggctgtgcg tgcatgggat cccaaaaaaa gaagatcgcg tcaattttct cttctgcgat
    15961 cagcgcacca agctgttgat cgcccccgag cgggcctgat ttcagacgca gcaggtttcc
    16021 cggcgctcgt ccctgttttt cgcttaccag ctgttcgatg agggtcccgg ttgtacccgt
    16081 gctggtcaaa tcgtgggcgg ccagtaccgg ccagtgcaaa cccacccagt cgatgagttc
    16141 agccttgcag ttgtcgtgtg cgacgagtgc gatgcgtttt ttctgcgcca tttatcctaa
    16201 tcacctttac acagtggccg gaaaaccatt gttttccacc aatgctttgg ctagtagccc
    16261 tgatggtcgt tattttcgtc atcggccact acggcctgcc tgagcaggtg aatccttcgg
    16321 cctaccagga aatgccgagc gcgatcaaga tcatgcgcgg cgccgaacct ttccggctgc
    16381 agggtaagac agacgtcggc tttctgctgg tgcacgggtt cgaagactct ccgtacacga
    16441 tgcggaacat cggcaacctg ctgcacagcg aagggcatac cgtgatagct ccgctgctgc
    16501 cgggccatgg taccaatatt
//
LOCUS       DAOQJF010000022        16055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1729_length_16055_cov_5.059375, whole genome shotgun sequence.
ACCESSION   DAOQJF010000022 DAOQJF010000000
VERSION     DAOQJF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 16055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16055
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1729_length_16055_cov_5.059375"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLF85_02225"
     CDS             <1..858
                     /locus_tag="PLF85_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNE18501.1"
                     /translation="ETLHAVAMAREKNIAECVLVGEEAVMKSTAEKEGISLAGVEIVN
                     VPDPVKAGMAAVQLVSEGKAQIFMKGYIHSDDFLRCVLNKEVGLRADNLLNHIFILEA
                     THLGRLLFITDGAMNIAPTLEEKARIISNTVALAQIFEIERPKVAVVSAVEVVTPGIQ
                     STLEAAALAKMSDRNQFHGGIVDGPLALDNAINPLAAKYKKIGGEVAGNADILLMPDI
                     VSGNALAKSFAHITGGTTAGLIFGAKAPVILPSRSDSKQSKLMGMACAVYVAGANAQK
                     RVKIGKVHF"
     gene            887..2071
                     /locus_tag="PLF85_02230"
     CDS             887..2071
                     /locus_tag="PLF85_02230"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNE18502.1"
                     /translation="MKKILVINSGSSTIKADVFAVKEGVPVFLAKALADKLGKSDALL
                     SIKDEQKKLREMAIAGLDVPAALERLFAELADAGICRADELVAIGHRVVHGGSRYTAP
                     VVVDDAVIAAIRGMAVFAPLHNPANLAGIEHCRRSTTVPQVAVFDTAFHQTMPAVAST
                     YAIPRELSEKYGIRRYGFHGTSHEYVTRECARVMGVGYAEFNSISLHLGNGASACAVR
                     SGKSIDTSMGFTPLEGLVMGTRSGDLDPSLPFFLQNVVGLTAADAENLLNKKSGLLGL
                     SGVSDMRELLSRADAGDGHAALARDIFCARIRKYIGAYLVWGRPHAIIFTGGIGENSP
                     EIRRRVLESLEHLDIHISIERNQELPADGKISRDEGVAVYVVSTDEELMIAQQALWLI
                     TP"
     gene            complement(2085..2363)
                     /locus_tag="PLF85_02235"
     CDS             complement(2085..2363)
                     /locus_tag="PLF85_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18503.1"
                     /translation="MIFFEILGVSARVDKVYIAGSEKFVGLITAALIPFVNKLSRPLD
                     AEIIARAAVGAVINVAGFWQLNGFKPDKKKIADNIAGVISVLGEKLAV"
     gene            2371..2817
                     /locus_tag="PLF85_02240"
     CDS             2371..2817
                     /locus_tag="PLF85_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE18504.1"
                     /translation="MRGSLSRPEAAVANLSSSQGILPLPFLIPYCTTKFAVRGFSDTL
                     RTEHKVRGIDNVTVHTVHPGAVATNITVNADRHNSNTEFFHQMLQKQGTTPDEAANII
                     LNGIAKNNPRIFINNGGVQDVLQRITPEHSADLVSLGMRLAGVKPR"
     gene            complement(2888..3262)
                     /locus_tag="PLF85_02245"
     CDS             complement(2888..3262)
                     /locus_tag="PLF85_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNE18505.1"
                     /translation="MEDIAARFILLVSGIFVVVDPIGTIPLMMSLTGGHSQQETRAVI
                     RRASFFGAAFLLLFALVLITAIAVVFFISYVILRASLRVQRLLGANGLMVLGRLMRLF
                     LAAMAVQFVFEGAIKLVASFKT"
     gene            complement(3271..>3606)
                     /locus_tag="PLF85_02250"
     CDS             complement(3271..>3606)
                     /locus_tag="PLF85_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNE18506.1"
                     /translation="IRASEMQYEPKELHLKAGVPVHLTLVNDGKVLHDFNLQGIKDSG
                     KPAHNHQVGHNHASGHNHAMAGSDSHIAVEPGKSAGLHFTPSAGEFAFYCSVPGHREA
                     GMSGKILAH"
     assembly_gap    3607..3706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3923..4126
                     /locus_tag="PLF85_02255"
     CDS             3923..4126
                     /locus_tag="PLF85_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNE18507.1"
                     /translation="MKNIELKIEGMTCAHCERTVEEAIREAGAVGKASRANGSATVSY
                     DETVVDLQKIREAIEENGYSVKN"
     gene            4135..6321
                     /locus_tag="PLF85_02260"
     CDS             4135..6321
                     /locus_tag="PLF85_02260"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNE18508.1"
                     /translation="MSQSKTLDISGMTCANCALRIEKGLSKLPGVQSATVNFAMETAS
                     VQFDEAVCELPQLIGKVEQLGYGAAEQKEDEADQRAEERHRDYVKTFRLWLVSAALSL
                     PLAYTMVAHLGISFLPMPELLMNPWFQLALAAPVQFIIGARFYAGAWRALMDKSANMD
                     TLVALGTSAAFFYSLWMSFTHGAHAQLYYETSAVLVTLILLGKLLEARAKGKTSSAIS
                     KLMHLQAKSAIVVRDGQELEVPIEKVRVGDLVLVKPGETIPVDATVVEGESAVNEAMI
                     TGESMPVEKKAGDIVIGATLNQLGMLRIRAEKVGKDATLARIVRAVKEAQGARAPIQR
                     FADRVSAVFTPTVLAAAIVTFVAWYFVVEAGNFAKALEHAIAVLVIACPCALGLATPT
                     SIMAGSGRAAESGVLFRNAVHLEHAGAIEVVALDKTGTITRGEPKVMALVSAQNEQDL
                     LMVAAAVEKLSEHPLAQAIVAEAKSRNLTLTEVQNFRVFPGGGVEASYQGKTVVIGSP
                     RYLSERSLSLTTYAEKAASLEAEGQTVVAVAMNGETLGLIAIADEIRPEARAVIAELA
                     QLGVETVMITGDNERTAAAIAAKAGISRYYAGVKPEGKAGIIAELKAAGKLTAMTGDG
                     INDAPALAAADVGIAMGGGSDIAIETADVALLGNDLHGLVRAIRMSRLTMRNIRQNLF
                     WALAYNTVGIPVAAAGFLAPWLAGAAMALSSVSVTLNALRLQRVKL"
     gene            6369..6704
                     /locus_tag="PLF85_02265"
     CDS             6369..6704
                     /locus_tag="PLF85_02265"
                     /inference="COORDINATES: protein motif:HMM:NF007494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNE18509.1"
                     /translation="METITYKDFEKLEIRVGTIVKAEEFSDARKPAYKLWIDFGSFGT
                     RKTSAQITQLYDLTNLVGRQVVAVTNFPPKQVGKFMSECLVLGGVGEAGQVVLLQPER
                     SVENGLRVL"
     gene            complement(6675..7724)
                     /locus_tag="PLF85_02270"
     CDS             complement(6675..7724)
                     /locus_tag="PLF85_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18510.1"
                     /translation="MNAGWLKSARFDVSLIAGVALLAAAMATTTVVWPALFLPVLAVH
                     TWLFGYEHLWATYTNLVVHPDDRTRYWRTIWLVPPVLLLLLYFTGRSYGMTTLLVVYF
                     FGQFFHTVRQSWGLAQQYRHRAGSMQWDNPRLSELTLWSVPIWGFLNRCSERPDEFIF
                     QEFWLPYVPRTIVIAAGIVCAILWTHWLVTRIIAYQRRELAWGHTLYMASHALVYLGG
                     YIVINELCSGWLLVNVWHNVQYMLFVWLFNNRRFAAGIDEKARALSWLSQGGRLRIAL
                     YSAATIALALPIYYLLPVIGTSFDDLVKNTAVPTAMVVGFTLTFHHYVADGVIWKRKN
                     NPQMTLLQNAQAVFH"
     gene            complement(7728..7958)
                     /locus_tag="PLF85_02275"
     CDS             complement(7728..7958)
                     /locus_tag="PLF85_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18511.1"
                     /translation="MTQKKLRLLLASCCLAATAISAAAPYQPPFDPKRKKPGEECKSN
                     EECQRHHKCEIKEEKGVCTAPPAPKLPPGVVT"
     gene            complement(7955..8218)
                     /locus_tag="PLF85_02280"
     CDS             complement(7955..8218)
                     /locus_tag="PLF85_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18512.1"
                     /translation="MKRNTVLFLILAGTVALAGSAVYAKQRAEAIMADEGPPEPPDPK
                     KKRPGEECSRDDECQKHHSCKKTGDKGMCTAPPRPKLPPGVVT"
     gene            8327..8530
                     /locus_tag="PLF85_02285"
     CDS             8327..8530
                     /locus_tag="PLF85_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNE18513.1"
                     /translation="MPYVNIQITRGATREQRSQLVKEVTDSLVRILGKKPEHTHIVIQ
                     QVEEDHWGFCGMLTEDYKKQNPG"
     gene            complement(8541..9407)
                     /locus_tag="PLF85_02290"
     CDS             complement(8541..9407)
                     /locus_tag="PLF85_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE18514.1"
                     /translation="MAIAYIYLIASAVLWGGVFHVIHYPLSVAPPFVLLTARFSLTAL
                     LLLPWLLIRHNYRLALGRRNFYDLLFLSVVGICGYNTVFTYGMTLTEPATGALIIAAN
                     PAMTTLIARIWKKERISPVRWLGIATAFAGLTFIVFKGDLGNALQLRLAPGNLVLLGA
                     PFAWAVYSVKSREVLQRMPTTVVTAALILLSLPPQAALAAVQLDGWRWAVEPAFWWPV
                     LYLGIFATGVSYLCWNKGVELIGAARSSVFVNLIPVTTLLVSLLLGQPLNTYHYIGGL
                     IVVAGVILATRR"
     gene            complement(9411..9785)
                     /locus_tag="PLF85_02295"
     CDS             complement(9411..9785)
                     /locus_tag="PLF85_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18515.1"
                     /translation="MAKSVAFFGSSDSEFVELSHVESNGQSQQLQVRYENDGTERWRE
                     IISVSRDELVQLAQWLDDLWDECPPATLAQGRLQFIFSHREHIGEGYYRVEWDGRLVL
                     SAYEQLGSSNKLRADQVRQLAG"
     gene            complement(9778..10299)
                     /locus_tag="PLF85_02300"
     CDS             complement(9778..10299)
                     /locus_tag="PLF85_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNE18516.1"
                     /translation="MNILLRNYGIAFVLTVASVFVNHLLGFNTERHETGEYARNAVTA
                     IFWCSIFLTVVQIRAASGNEHSFMHAFRNGSLYSVLYSASFSLFMVFYQKVVNPRFYP
                     TYREFFEARLKAAQIAPDLMAVKMRQFDMTFNGDFPTYVLLFLYMAMGGVIMAAIAGA
                     IFRNPVKPRPSRG"
     gene            complement(10296..12011)
                     /locus_tag="PLF85_02305"
     CDS             complement(10296..12011)
                     /locus_tag="PLF85_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE18517.1"
                     /translation="MHHGLLADLGIAIVIAATMTVVMHFLKQPLILGYLIAGVLIGPY
                     MTPQLVTDVHNIDTISEIGLVLLLFIIGLELSPQSLMANGRAIFITGSLQFPFNLAVI
                     LFLVGAAGHFVFWQFGRLEPLYIAVFCSLSSTAIVVKSLYDKFELESIAGKLSIGILI
                     FQDLWAIFFLVLQPNLNNPEVSLITIALLKAVLLLAAGFYFSKYVLSALFNRIAKNPE
                     LVVSLSIGWCALLASVASLLGLSIEMGALIAGFAISIFPYHIHITARVSPLRDFFMTL
                     FFISLGMKIPAPRAELLPPLLAILAAVVLSKLLVVTPLVRLAGGSTRAGILTALNLGQ
                     VSEFSLVIAAVAVKYGHISEKLMSVLVYALGISAVTSTYTIKYNIRIWRLLRRIFGRL
                     FFLQLHDEREFEHAAAGEVPPVFILGFHRGARAIIDNLRESDPGFLSSIRVIDYNVEV
                     LKALSVYGIQGIFGDISSVETLKHCGIDRAKLIVSTVPDLLLKGTSNFKLVRACRELN
                     PAAVIFATAETPDQVRRLEDAGADHVVLPYSLVGQVLAEQILAISAGEFDEKQESTQF
                     QGEPT"
     gene            complement(12015..12536)
                     /locus_tag="PLF85_02310"
     CDS             complement(12015..12536)
                     /locus_tag="PLF85_02310"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HNE18518.1"
                     /translation="MLSRCVIFAILLPAFSGCVPQNNDAPIGEEIPAPENLKSIAAGE
                     VVYVPAYARIHHLKLKDRTAALVTTLAIHNTDMKHPIVLRTVNYYAENGSLLRSYVRR
                     PRLLPPLATAIYYLAPEETAKAGIGANAIVEWVAEENVAKPLIESIMVSTDHTLGISF
                     MSQGYKIRSVAGK"
     gene            complement(12540..13193)
                     /locus_tag="PLF85_02315"
     CDS             complement(12540..13193)
                     /locus_tag="PLF85_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNE18519.1"
                     /translation="MTDLASAKATLKDAIRKFSYRENHEQPFTLASGKISPYYFDLKQ
                     TLLQPGYLALAGEVVAGIIAQHYQGKVIAAGLTMGADPIIYAACLAHGGAGVDGGQDA
                     LQAGDLAGMDGSTTLTNHSLRLIPAIVRKEAKDHGTGKKIEMLKGIDTTTPCVMVDDV
                     ITTAGSTLKAFAAMREAGFTVRHAICILDRGEGGREALLSNGVELLPVFTAAEFREK"
     gene            complement(13239..13742)
                     /locus_tag="PLF85_02320"
     CDS             complement(13239..13742)
                     /locus_tag="PLF85_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE18520.1"
                     /translation="MSAALRLYALRGVEKTSLRDLAAALHVDPMALYHYFPNREKLLA
                     AALEQAFEALPQKMPEGCTARAAATRLLLAYLDTAKKHRSLTVHLLSRQDAWPTNLVR
                     FNEALLQALGQHCQNTPLALRLRDILIDYTHGYLLAAGNFSVGGGKQAQAEFTNSVEL
                     ILNAGQT"
     gene            13769..13984
                     /locus_tag="PLF85_02325"
     CDS             13769..13984
                     /locus_tag="PLF85_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE18521.1"
                     /translation="MRAGRVYVDGGRLISSFLAAGLVTDLTLTRIPILLGEGLPLFSG
                     LPNRLPLQHIATRTWSNGFVQSSYGAG"
     gene            14070..14459
                     /locus_tag="PLF85_02330"
     CDS             14070..14459
                     /locus_tag="PLF85_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18522.1"
                     /translation="MRKIFFCTLFVLPLFAAGKSETFSGTILSTEEDFCGKSVDYVLQ
                     ASLATESRGNLVLVLAPKWYMVRLKMEIRIGDKVEATVLRQSDGKFHVLTMKVGGKTF
                     RLRDAKGVPLWKPEPGSEDLFKSICKA"
     gene            14576..15166
                     /locus_tag="PLF85_02335"
     CDS             14576..15166
                     /locus_tag="PLF85_02335"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNE18523.1"
                     /translation="MIAVGKKAPDFKATALVGKDFKDISLSDFKGKYVVLFFYPLDFT
                     FVCPTEIIAFSDKAAEFEKLGAQVVGCSVDSKFSHLAWSEVPREKGGIGEIKYPLLAD
                     ITKDIARSYGVLIEEAGIALRGVFIIDGNGVLKSATINNNNVGRNIDEVLRTVQADQF
                     AESHPGEVCPANWTPGKEAMKADVKGSKDYFSKHGK"
     gene            15251..>16055
                     /locus_tag="PLF85_02340"
     CDS             15251..>16055
                     /locus_tag="PLF85_02340"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNE18524.1"
                     /translation="MYSYSNNQLQWNKQALAQLLPHLADRLRSEYQYTGPFWLYQEDI
                     LKERLRLLEEGLPGCSFYYSVKSQSNLTLLSLIASHTRFGADVVSGGELYRALKAGFT
                     PDKIVFAGVGKTEAEIHAALDLRIRSFHVESIAELKDIARIAREKGLTAQVSLRLNPD
                     VEVDTHRHITTGKEENKFGMSEADVGEAIAILKNEPGLKLIGLQAHIGSQIFDVEPYI
                     KTAKYMNAKRELLAAQTGAKIEYISLGGGFGVDYKQLDGSPTSFDFGTLS"
BASE COUNT         3602 a         4335 c         4671 g         3347 t
ORIGIN      
        1 gaaacactgc acgccgtcgc gatggcgcgt gaaaagaaca tcgccgagtg tgtgctggtg
       61 ggcgaagaag cggttatgaa atcgactgct gaaaaagaag gcattagcct tgctggagtg
      121 gagatcgtga acgtgccaga ccccgtgaaa gccggaatgg ccgcggtgca gttggtgagt
      181 gagggcaagg cgcagatctt catgaagggc tacatacaca gcgacgactt cctgcgctgc
      241 gtccttaata aagaggttgg cctgcgtgcc gacaaccttc tcaaccatat ctttattctc
      301 gaggcgacgc atctgggtcg gttgctcttc atcaccgatg gtgccatgaa tattgcaccg
      361 acgttggaag agaaggctcg catcatcagc aacactgtcg cgctggcaca aatcttcgaa
      421 attgaaagac ccaaggtcgc ggtcgtctct gccgtcgagg tcgttacccc gggcatacag
      481 tcgacgcttg aggcagcagc actcgcgaag atgagtgacc gtaaccagtt ccatggtggc
      541 atcgtcgatg gcccgctggc gctcgacaat gcgatcaacc cgctagcggc gaaatacaag
      601 aagatcggcg gtgaagtggc tggcaatgca gatatacttt tgatgcccga cattgtttcg
      661 ggcaatgcgc tcgcgaaatc gttcgcgcac attaccggtg gcacaactgc agggctcata
      721 ttcggcgcga aggccccggt gatcttgccc tcgcgctccg attcgaagca gtcgaagctg
      781 atgggcatgg cctgcgcggt gtatgttgcc ggtgcaaatg cacagaagcg agtcaaaata
      841 ggcaaggtgc atttctgacc ggcaaagccg gtcagaaatg ctaactatga aaaaaattct
      901 ggtcatcaac tcgggctcgt cgaccattaa ggccgatgtg tttgcagtca aagagggagt
      961 tcctgtcttt ttggcgaaag cactcgctga taaactgggc aaatctgacg ccctactgtc
     1021 cattaaagat gaacagaaga agctccgcga aatggcgatc gcagggctcg atgtgccggc
     1081 ggcactcgaa cggctctttg cggagcttgc cgatgccggt atttgccgtg ccgatgaact
     1141 ggtcgctatc ggccaccgcg tggtgcatgg cggtagccgc tacaccgcgc ctgttgtcgt
     1201 cgacgacgcc gtgattgcgg ctattcgcgg gatggcggta tttgcgccgc tgcacaaccc
     1261 ggcgaacctc gcaggcattg aacattgccg gcgcagcaca accgtaccgc aggtggctgt
     1321 ctttgatact gcgttccacc aaacgatgcc cgcggtggcc tcgacctatg ccataccgcg
     1381 cgagctgagc gaaaagtatg gtattcgccg ctacggcttt cacggtacgt cgcacgaata
     1441 cgtcacccgc gagtgcgcac gtgtcatggg tgtcggttac gcagaattca actctatctc
     1501 gctgcacctg ggtaatggtg caagcgcgtg cgcggtgcgc agcggcaaga gcatcgacac
     1561 gagcatgggg ttcaccccgc tcgaagggct ggtcatgggc acgcgcagtg gcgacctgga
     1621 cccttcgctg ccgtttttcc tgcagaatgt ggtcggtctc acagcggctg atgcagaaaa
     1681 tctgctcaac aaaaagtcgg gcctcttggg gttaagcggc gtgagcgata tgcgcgaact
     1741 gttaagccgc gccgatgccg gcgacgggca tgcggcactc gcgcgtgaca tcttttgcgc
     1801 gcgtatacgc aagtatatcg gcgcttacct ggtatggggc cggccgcatg cgattatctt
     1861 caccggcggc atcggcgaga attcaccaga gatccgccgc agggtcctgg aaagcctgga
     1921 acacctcgat atccatatat cgatcgagag aaaccaggag ttgcctgccg atggtaaaat
     1981 cagcagggac gaaggtgtcg ctgtctacgt ggtcagtacc gacgaagagc tgatgattgc
     2041 gcagcaggcg ctgtggctca taacgccgta aaatatcgaa acgctcagac cgcgagtttt
     2101 tcgccgagta cagagatcac tccggcaata ttgtcggcga tcttcttctt atctggctta
     2161 aaaccgttga gctgccaaaa accggccacg ttgatcacgg ccccgacagc tgctcgcgcg
     2221 atgatttcgg cgtcgagcgg tctggagagt ttgttcacga aaggtatcag cgcagcggtg
     2281 atgaggccga cgaatttttc tgaacctgca atatagacct tgtcgacccg cgccgatacg
     2341 ccgaggatct cgaaaaaaat cacgcggccg atgcgcgggt cgctttcgcg ccccgaagcg
     2401 gcggttgcca acctttcttc gagccagggt attctgccgc tgccgttcct gattccctat
     2461 tgcaccacaa aatttgccgt acgcggcttc agcgacacgc tgcgtaccga gcacaaggtg
     2521 cgtggtatcg acaatgtcac cgtgcataca gtgcatccgg gtgccgtcgc cacgaacatc
     2581 acggtgaacg ccgaccgcca taacagcaac accgaatttt tccaccagat gttgcagaag
     2641 cagggaacca cccccgacga agcagcaaat attatactca atggtattgc aaaaaacaat
     2701 ccgcgcatct tcattaataa tggcggagtt caggatgtgc tgcagcgcat tacccccgag
     2761 cattcggctg acctggtatc gctggggatg cgactggccg gggtgaagcc tcgctgacct
     2821 tgcttgtgca ggtcttttcg gtgccgtgtc ctattggtgc gccatatatg gcgcaccaac
     2881 aggaccatca agttttaaag ctggcgacca gcttgatcgc tccctcgaac acaaattgca
     2941 cagccatcgc tgccagaaaa agtcgcatta gcctgcccag aaccatcagg ccgttggcgc
     3001 ccaatagccg ctgcacacgc aggctggcgc gcaagataac gtagctgata aagaagacga
     3061 cggcgatggc agtgatgagt accagcgcaa acagcagcaa gaaggctgca ccgaaaaaag
     3121 aggcacgccg gatcacggcg cgggtctcct gctgtgagtg gccgccggta agcgacatca
     3181 ttaacggaat cgtgccgatg gggtcgacaa cgacgaagat tcctgaaacc agcagaatga
     3241 aacgcgccgc gatatcttcc attgcctgcc tcagtgcgcg agaatcttgc cgctcatgcc
     3301 ggcttcgcgg tgccccggta cgctgcagta gaaagcgaac tcccccgccg agggtgtaaa
     3361 atgcaggcca gccgatttgc cgggttcaac cgcaatgtgg ctgtcgctcc ctgccatggc
     3421 gtggttatga cccgacgcat ggttgtggcc aacctggtgg ttgtgtgcgg gtttgccgct
     3481 gtcctttatc ccctgcaaat tgaagtcgtg cagtaccttg ccatcattta ccagcgtcag
     3541 gtgtactggt acaccagctt tgaggtgcag ctctttgggc tcgtactgca tctctgacgc
     3601 cctgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncacc agctttgagg
     3721 tgcagctctt tgggctcgta ctgcatctct gacgccctga tagacaccga agcgaagtag
     3781 acattacacc cgaggtggaa aaaattttgt gatattttcg gacaggcggt gtaatatctg
     3841 ccatagcgga tggtcatgtt ggcaacggga gcgatcgagg tgaaagcctg aaggaacccc
     3901 gtgcaacaca atggaggatt ttatgaaaaa tatcgaactg aaaattgagg gcatgacctg
     3961 tgcacattgc gagcgtaccg tcgaagaggc gattcgcgag gcgggtgctg ttggcaaggc
     4021 gagcagggcg aacggttcgg cgactgtcag ctatgacgag accgtcgttg acctgcagaa
     4081 aatccgcgag gccatcgaag agaatggcta ctcggtgaaa aactaaaggc ctctatgagc
     4141 cagtcaaaga cgctcgatat ttcgggcatg acctgcgcca actgcgcgct gcggattgaa
     4201 aaaggtttgt cgaaactacc cggtgtacag tcggcgacgg tcaattttgc aatggagacc
     4261 gcttcagtac aattcgatga agcggtgtgc gaactgccgc agctgatcgg caaggtcgag
     4321 cagctgggct acggcgccgc tgaacaaaag gaagacgagg cagaccagcg cgccgaagaa
     4381 cgccaccgtg actacgtgaa gacgtttcgg ctgtggctgg tttcggccgc gctgtcgctg
     4441 ccgctggcct ataccatggt cgcgcacctc ggcatcagct tcttgccgat gcccgagctc
     4501 ctcatgaacc catggttcca gctggcactc gccgctccgg tgcagtttat catcggcgcg
     4561 cgcttctatg ccggtgcctg gcgcgcgctc atggataaga gcgccaacat ggacaccctg
     4621 gtggcgcttg gcacctcagc cgcgttcttc tacagcctgt ggatgagttt cacgcatggc
     4681 gcgcacgcac agctctacta tgaaacaagc gccgtactcg tgacattgat tctgctcggc
     4741 aaactgctcg aagcgcgtgc gaagggcaaa acctcttcgg cgatcagcaa actcatgcac
     4801 ctgcaggcaa agagtgcaat cgtcgtgcgc gacggccagg agctcgaagt accgatcgaa
     4861 aaggtacgcg tcggcgatct cgtgctggtg aaacccggcg agacgatacc cgtcgatgcg
     4921 accgttgtcg aaggcgaatc ggctgtaaat gaagcgatga tcacgggcga aagtatgcct
     4981 gtcgaaaaga aggcgggcga tatcgtcatc ggtgcaacac tcaaccaact cggcatgctg
     5041 cgcattcgcg ccgaaaaggt cggcaaagat gccaccctcg cgcgcatcgt gcgtgccgtc
     5101 aaagaagcgc agggtgcgcg cgcgccgata cagcgttttg ccgaccgtgt ttctgcagtg
     5161 ttcactccga ctgtgcttgc cgctgccatt gtgacctttg ttgcctggta tttcgtggtt
     5221 gaagcaggca actttgcgaa ggcactcgag catgcgattg cggtgctggt gattgcctgc
     5281 ccctgtgcgc tcggtcttgc gaccccgacg tcgatcatgg cgggttcggg ccgcgcggca
     5341 gaatcgggtg tgctgtttcg caacgccgtg catctcgaac acgccggcgc gatcgaagtg
     5401 gtcgcgctgg ataagactgg cacgatcacc cgcggtgagc cgaaggtaat ggcgctcgtg
     5461 agcgcgcaga atgaacaaga tctgctcatg gttgcggcgg cggtggagaa actcagcgaa
     5521 catccccttg cgcaggcgat tgtcgccgaa gcgaagagtc gcaacctgac cctgaccgaa
     5581 gtacagaatt ttcgcgtctt tcccggtggc ggtgtcgaag cgtcatacca gggtaaaacc
     5641 gtcgtgatcg gttcgccgcg ttatctgtcg gagcgttccc tgagcctcac aacttatgcc
     5701 gagaaggcag cgtcactcga agccgaagga cagactgtcg tcgcggtggc gatgaatggc
     5761 gaaaccctcg gcctcattgc gattgctgac gagatacgcc ctgaagcacg cgcggtgatt
     5821 gccgaactcg cacagctggg tgtagagact gtcatgatca caggtgataa cgaacgtacc
     5881 gcagcggcga ttgcagcgaa ggcgggcatc agccggtact atgcaggggt aaagcctgaa
     5941 ggtaaggcgg gaatcattgc cgagctcaag gcagcgggca aactcaccgc gatgactgga
     6001 gatggcataa acgacgcacc agctctcgct gcagccgacg tcggtattgc aatgggcggt
     6061 ggttcagaca tcgccatcga aacagctgac gtggcactac tcggcaatga cctgcatggc
     6121 ctcgtgcgtg ccattcgcat gtcgcggctg acgatgcgca acattcggca gaacctgttt
     6181 tgggcgcttg cctacaacac ggttggtatt ccggtcgcgg ctgcgggttt tctcgccccc
     6241 tggctcgccg gtgcggcgat ggcgctgagt tcggtctcgg tgacgctcaa tgcgctgcgc
     6301 ttgcaacggg tgaagttgtg aataaatcgc ccggctttag agccgggcgc ggtctaatat
     6361 tcaactatat ggaaacgatc acatacaaag actttgaaaa gctcgaaatt cgcgtgggaa
     6421 ccatcgtgaa agccgaagag ttttccgacg cgcgtaaacc cgcctacaaa ttgtggatcg
     6481 acttcggcag ctttgggacc cgaaagacca gcgcgcagat cacccagctc tacgatttaa
     6541 caaacctcgt cggcaggcag gttgtcgccg tgacgaactt tcctcccaag caggtcggca
     6601 agttcatgtc ggaatgtctt gtgctggggg gcgtcggtga ggcaggccag gtcgtgctgt
     6661 tgcagcccga gcggtcagtg gaaaacggcc tgcgcgttct gtagcagagt catctgcggg
     6721 ttgttcttcc ttttccagat caccccgtcc gccacgtagt ggtgaaaggt cagcgtgaag
     6781 ccgacgacca ttgctgtggg aaccgcggta ttcttcacca ggtcatcgaa gctggtgcct
     6841 atcaccggca gcaggtagta gatcggcagc gccagtgcaa tcgttgcagc actgtagagg
     6901 gcgatacgca atctaccgcc ctgtgaaagc cacgagagcg cacgtgcttt ttcgtcgatg
     6961 ccggcagcga agcgccggtt attgaagagc cagacaaaga gcatatactg cacattgtgc
     7021 cagacattga ctaacagcca gcccgagcag agctcgttga tgacgatata accgccgagg
     7081 tagaccagcg catgcgaagc catgtagagc gtatgccccc acgctaactc tctgcgctgg
     7141 taggctatga tgcgcgttac gagccagtgt gtccaaagaa ttgcacagac gatgcctgcg
     7201 gcgatgacga tggtacgcgg cacgtagggt aaccagaact cctggaagat aaactcgtcg
     7261 ggccgctcag agcagcggtt caaaaatccc caaatgggca ccgaccagag cgtgagctct
     7321 gaaagccgtg ggttatccca ctgcatgctg ccggcgcggt ggcggtactg ctgcgcaagg
     7381 ccccagctct gccgcacggt gtggaagaac tggccgaaga agtagacaac gagcagcgtg
     7441 gtcatgccgt agctgcggcc tgtgaagtag agtaacagca gcagcaccgg tggaaccagc
     7501 caaatcgtgc gccagtagcg cgtgcgatca tcggggtgta cgaccaggtt cgtgtacgtc
     7561 gcccacaggt gctcgtagcc aaagagccac gtgtgtactg caagcaccgg caagaagagc
     7621 gcgggccaga caaccgtcgt cgttgccatg gcagcagcca aaagcgcaac gccggcaatc
     7681 aggctcacgt caaaacgagc gctcttgagc cagccagcgt tcatgattca ggtcaccaca
     7741 cccggcggta acttgggcgc tggtggtgct gtacagacgc ctttctcttc tttaatctca
     7801 cacttgtgat ggcgctggca ttcctcgttg ctcttgcact cttcgccggg ttttttgcgt
     7861 ttcgggtcaa acggcggctg gtacggcgca gcagctgaga tagcagttgc ggcgaggcag
     7921 cagctagcga gcagcaggcg gagtttcttc tgcgtcatgt cacaactcct ggtggcagct
     7981 tcggccgcgg tggtgctgta cacataccct tgtcgccggt cttcttgcag ctgtggtgtt
     8041 tctggcactc atcgtctctg ctgcactctt cgccaggtct tttcttcttt gggtctggcg
     8101 gttccggtgg gccttcatcg gccatgattg cttcagcgcg ttgtttggca tacaccgcag
     8161 accccgcgag tgcgacagtg ccggcgagta taagaaacag aacggtattt cttttcatgt
     8221 tgctcctctc tctgatttca tttggacgcc gcatgctgtt tgaaaggtca atttattttg
     8281 ctgtcggctt gcattgacgc cttgtggcaa ccccgtagcg gggaaaatgc cttacgtcaa
     8341 tatccagatt accaggggcg caacgcgcga acagcgctcg cagctggtca aagaagtcac
     8401 cgattcgctg gtgcgtattc tcggcaaaaa accggagcac acgcacatcg tgatacagca
     8461 agtcgaagaa gaccactggg gtttctgcgg catgctgacc gaagactaca agaagcagaa
     8521 tcccggctga acctgcagga ttacctgcgg gttgcgagta tgacacccgc tacaacgatt
     8581 aatcccccga tgtagtggta ggtattgagc ggctggccca gtagtagcga cacgagcagt
     8641 gtggtgaccg gtatcaggtt gacaaatacc gaactgcgtg ccgcgccgat gagttcaaca
     8701 cccttgttcc agcaaaggta tgagacacct gtggcgaaga tgccgaggta gagcaccggc
     8761 caccaaaatg cgggttcaac cgcccagcgc cagccatcga gctgcaccgc cgcaagtgct
     8821 gcctgtggcg gtagggagag caggatgagc gctgcagtga cgaccgtcgt cggcatgcgc
     8881 tgcagtactt cacggctttt caccgagtag acggcccagg caaacggggc ccccagcaag
     8941 acgaggttac ccggtgccag ccgcagctgc agagcattac ccaggtcgcc cttgaacacg
     9001 ataaaagtca ggcctgcaaa agcagtagca atgcccagcc agcgtacggg tgagatgcgc
     9061 tcttttttcc agatgcgcgc aatcagcgtg gtcatcgcgg gattcgccgc gatgatcagt
     9121 gcgccggttg caggttcggt cagcgtcatg ccataggtga agacggtgtt ataaccgcag
     9181 atgcccacca cagagagaaa aagcaggtcg taaaaattcc tgcgcccgag tgcgaggcgg
     9241 tagttgtgcc gtataagtag ccacggcaaa agcagtaacg cagtcagcga aaatcgtgcg
     9301 gtgagcagca cgaaaggcgg agcgacagac agtgggtaat gtatcacatg gaaaactccg
     9361 ccccaaagta cggcgctggc aataaggtag atgtacgcaa tcgccatggt tcagccagcg
     9421 agttggcgca cctggtcagc acgcagtttg tttgagctgc ccagctgttc ataggcagaa
     9481 agcaccaggc ggccatccca ttcaacgcgg tagtaacctt cgccgatatg ttcacggtgg
     9541 gaaaaaataa actgcagcct gccctgtgcc agcgttgccg gcgggcactc gtcccaaagg
     9601 tcatccagcc attgtgccag ttgtaccagt tcgtcgcggc tgaccgaaat aatttcacgc
     9661 cagcgttcag tgccgtcatt ttcatagcga acctgtagtt gctgcgactg gccgttactt
     9721 tccacgtgac tgagttctac gaactcgctg tccgagctgc caaagaaggc gacggattta
     9781 gccacggctt ggcctgggtt ttaccgggtt tctgaatatt gcccccgcaa tcgccgccat
     9841 gatgacaccg cccattgcca tgtatagaaa cagtaaaaca taggtgggga aatccccatt
     9901 gaaagtcatg tcgaactggc gcatcttaac ggccatcagg tctggtgcga tttgcgctgc
     9961 tttgaggcgt gcttcaaaaa actcgcggta ggttgggtag aagcggggat ttaccacttt
    10021 ctggtaaaat accatgaaga gggagaaact ggccgagtag agcaccgagt agaggctgcc
    10081 attgcggaat gcgtgcatga agctgtgttc attgccgcta gccgcgcgta tttgtacgac
    10141 tgtgagaaag atcgaacacc agaagatcgc cgtgacagcg ttacgggcat attcgcctgt
    10201 ctcatgccgc tcggtattga aacccagcag gtgattgacg aacacgctgg cgactgtcag
    10261 tacaaaggca attccgtaat tgcgaagaag tatattcatg ttggttctcc ctgaaattgt
    10321 gttgattcct gcttctcatc aaactcgccg gctgagatcg ccagtatctg ttctgccaga
    10381 acctggccga cgagagaata tggtagtacg acatgatcgg cgcccgcgtc ttccagccgg
    10441 cgcacctggt cgggggtttc agccgtcgca aagatgactg cagcggggtt taactcgcgg
    10501 caggcgcgta ccagcttgaa attgctcgta cccttcagca gcaggtcggg taccgtcgaa
    10561 acgatcagtt ttgcacggtc gatgccgcag tgtttgaggg tctcgacact gctgatatcg
    10621 ccgaagatgc cctgtatgcc ataaaccgac agggccttta gcacctcgac gttatagtcg
    10681 atgaccctga tgctcgagag aaaacccggg tcgctctcgc gcaggttgtc gataatcgcg
    10741 cgcgcgccgc ggtgaaatcc caggataaaa acgggcggta cctcaccggc tgcggcatgc
    10801 tcaaattcgc gctcatcatg cagctgcagg aaaaacagcc tgccgaatat cctgcgcagc
    10861 agccgccaga tgcggatgtt gtacttgatt gtataggtcg aggtcaccgc agaaatgccg
    10921 agtgcataca ccagtaccga cataagtttt tcactgatat gtccgtattt gaccgccacc
    10981 gctgcgatca ccagcgagaa ctcactcacc tggcccaggt tgagcgcggt gagtatgccg
    11041 gcgcgtgtac tgccgcccgc taatctcacg agaggggtaa cgacgagcag cttagagagt
    11101 acaaccgcag caagaatggc cagcagggga ggcaaaagct cggcgcgcgg tgcgggaatt
    11161 ttcatgccga gcgagataaa aaacagcgtc atgaaaaaat cgcgcagcgg agatacccgc
    11221 gcggtgatgt ggatgtggta ggggaaaatg gatatcgcga agcctgcgat gagcgcgccc
    11281 atttcgatcg aaagacccag cagcgaggca acagaagcga gcagtgcaca ccagccgata
    11341 gaaagagaaa ccaccagctc gggatttttc gcgatacggt taaacagcgc cgagagcaca
    11401 tacttactga agtagaaacc cgccgcgagc agcagcaccg ccttgagcag ggcgatggtg
    11461 atcaggctga cttcggggtt gttcaggttt ggctgaagca ccaaaaagaa tatcgcccag
    11521 agatcctgga agatgagtat gccgatcgaa agtttgcccg caatcgattc cagctcgaat
    11581 ttgtcgtaga gcgatttgac gacaattgct gtcgagctca gcgagcagaa gaccgcgata
    11641 taaagcggtt cgaggcgacc gaactgccag aagacgaagt gcccggcagc gcccacaaga
    11701 aaaagtatca cggcgaggtt gaacggaaac tgcagcgagc cggtaatgaa gattgcccgg
    11761 ccgttggcca tcaggctctg cgggctcagt tcaaggccaa tgataaagag cagcaggacg
    11821 aggccgatct ccgaaatggt gtcgatgtta tgcacatcgg tcacgagctg cggtgtcatg
    11881 tatggcccaa tcaacacacc ggcgatcagg tagcccaaaa tcagcggctg cttcaggaaa
    11941 tgcatcacca cggtcatggt cgccgcgatg acgattgcga taccgaggtc tgccagcagg
    12001 ccgtggtgca ttcgttattt gcctgcgacg gacctgatct tgtagccctg gctcatgaaa
    12061 gagatgccca gcgtgtggtc tgtcgagacc atgatcgatt cgatcaatgg cttcgcgaca
    12121 ttctcttcgg caacccattc gacgatggcg tttgcgccga taccggcttt tgccgtctct
    12181 tcaggtgcca ggtagtagat cgctgtcgcg agcggtggca gcaggcgggg ccgacgcaca
    12241 tacgagcgca gcagcgagcc gttctctgcg tagtagttta ccgtgcgcaa aacaatgggg
    12301 tgcttcatgt cggtgttgtg aatcgccagc gtggtcacca gcgccgcggt gcggtctttg
    12361 agcttgaggt gatggatgcg ggcgtatgcc ggcacgtaca caacttcgcc ggcggcgatg
    12421 gatttcaggt tttccggcgc gggtatttct tcgccgatcg gcgcatcgtt gttctgcggc
    12481 acgcagccgg aaaaagccgg cagcagaatc gcgaaaataa cgcagcgtga cagcataagc
    12541 tatttctctc tgaactccgc cgctgtaaag acaggcagta gttcaacgcc attcgacagc
    12601 agggcttcgc gcccgccttc gccgcggtcg agaatacaga tcgcgtggcg taccgtgaag
    12661 cctgcctcgc gcatggcggc gaaagctttg agcgtagaac ccgcggtggt gatcacgtca
    12721 tcgaccatca cgcatggtgt cgtggtatcg atgcccttga gcatttcgat tttttttccg
    12781 gtaccatggt ctttcgcttc tttgcgcacg attgccggta ttagacgcag ggaatggttg
    12841 gtgagcgtag tcgaaccatc cattcccgcc aagtcgccgg cttggagggc atcctgcccg
    12901 ccgtcgaccc ccgcacctcc atgtgccaga cacgctgcat agataatcgg gtctgcaccc
    12961 atcgtgaggc ctgctgcaat tactttgccc tggtagtgct gtgcgatgat acccgcaaca
    13021 acctcgcccg cgagcgccag gtatcctggc tgcaacagtg tctgctttag gtcgaagtaa
    13081 taggggctga ttttgcccga tgcgagcgtg aacggctgct cgtggttctc gcggtacgaa
    13141 aatttgcgta tagcgtcttt taacgtggct ttggccgatg caaggtcagt catcgtcggc
    13201 atgctcagcc ggcgacacag tcggaaaagc cttaaccctc aggtttgccc tgcgttgaga
    13261 atgagctcca ccgagttcgt aaattctgcc tgggcttgtt ttccgccgcc gacagaaaaa
    13321 ttccccgcgg cgagcaggta gccgtgcgta tagtcgatca ggatatcccg caggcgcagg
    13381 gcgagcggcg tgttctggca gtgttgcccg agcgcctgca gcagcgcctc attgaagcgc
    13441 accaggtttg tcggccaggc gtcctggcgc gagagcaggt gcacggtgag actccggtgc
    13501 tttttggcgg tatcgaggta tgccagtaga agcctggttg cggctgctct ggccgtgcaa
    13561 ccctcgggca tcttctgggg caaggcttca aatgcctgct caagcgctgc cgcgagcagc
    13621 ttttcgcggt tgggaaagta atggtagagc gccatcgggt caacgtgcag cgcggccgca
    13681 aggtcgcgca ggctggtttt ttcgacgcca cgcagggcat agaggcgcag ggctgcagac
    13741 agaatatgcc ggtgcgtcag cttcggttgt gagggccggg cgcgtctatg tggatggcgg
    13801 ccgcctgatc agctctttcc tggcggctgg actcgtgacc gatctgacgc tgactcgcat
    13861 accgatactg ctcggtgaag gcctgccgct tttttccgga ctaccgaacc gcctgccgct
    13921 gcagcatatt gcgaccagaa cctggtcgaa tggctttgtg cagagtagct atggagctgg
    13981 ctgagaagtc ccgtaaacta gcaggttcct ttttcaggag cctgttagaa actgctggca
    14041 ggtacatttt cattctgccg gttggccgta tgagaaaaat cttcttctgc acactattcg
    14101 tactaccact cttcgctgcc ggcaaaagcg agaccttcag cggcacgatc ttatctactg
    14161 aagaagattt ctgcggcaag tcggtagact atgtgctgca ggcctcgctc gccaccgaat
    14221 cgcgtggcaa cctcgtcctt gttcttgctc ccaagtggta catggttcgc ctgaaaatgg
    14281 agatcaggat cggcgataaa gttgaagcga ctgtactgcg gcagagcgac ggcaagtttc
    14341 atgtgctgac catgaaggtt ggcggtaaga cgttccgcct gcgcgacgcg aagggcgtcc
    14401 ccctctggaa accagaaccg ggcagcgaag acctttttaa gtcgatctgc aaggcctaac
    14461 gccgcgcagc aagtaagtcc tgcaggcagg tcttcaggtt tttttggtcg taccggcatt
    14521 tttgccatat ctttgacgta ctcaaaaaca atactgagta taatcgaggt taaacatgat
    14581 cgcagttgga aaaaaagctc ctgattttaa agcgaccgcc ctggtcggta aagatttcaa
    14641 agatatttcg ctgagcgact ttaaaggcaa gtacgtagta ctgttcttct acccgctcga
    14701 cttcacattc gtctgcccga cggaaattat tgctttctct gataaagcag ccgagttcga
    14761 aaaactgggg gcgcaggttg ttggctgcag cgtcgacagc aagttcagcc acctggcatg
    14821 gtcagaagtc ccgcgcgaaa aaggcggtat cggcgaaatc aaatacccac tgctcgccga
    14881 catcacgaaa gacattgcgc gtagctacgg cgtgctgatc gaagaagccg gcatcgcgct
    14941 gcgcggcgtg ttcattatcg acggcaacgg cgtgctcaag tcagccacga tcaataacaa
    15001 caacgttggc cgtaacatcg acgaagtact gcgtaccgtt caggcagacc agtttgccga
    15061 atcgcacccc ggtgaagttt gccctgcaaa ctggactcct ggcaaagaag cgatgaaagc
    15121 cgacgtgaaa ggttcaaagg actacttctc gaagcacggc aaataaccct tttagccctt
    15181 cggggccaag agttttacag catgaaggca tctgtgtttt ggaccagatg ccttttttat
    15241 gaagtgatct atgtacagct attcaaacaa ccaactccag tggaataaac aggcactcgc
    15301 acaacttctg ccccatctgg cagaccgtct gcgcagtgaa taccagtaca caggtccctt
    15361 ctggctttac caggaagaca tcctcaaaga aaggctgcgc ctgctcgagg agggtttgcc
    15421 aggttgcagt ttttattact cggttaagtc ccagtcaaac ctcacactgc tgtcattgat
    15481 tgcctcgcac acccgctttg gtgccgacgt cgtttctggc ggcgagctgt accgtgcgct
    15541 caaagccggt tttacgcccg acaagattgt atttgccggc gttggcaaaa ccgaagcgga
    15601 aatccatgca gcgctggatc tgcgtatccg cagttttcat gtcgaaagta tagcggagct
    15661 gaaagatatc gcccgtatcg cccgcgaaaa aggtcttact gcacaggtga gcctgcgcct
    15721 gaacccggac gttgaggtag atacgcatcg ccatatcaca acaggcaaag aagaaaacaa
    15781 atttggtatg agcgaggcag acgtaggcga agccattgct attctcaaaa acgaaccagg
    15841 cctgaagctc attggcctgc aggcacacat cgggtcgcaa atttttgatg ttgagcccta
    15901 catcaaaaca gcaaaataca tgaacgcgaa acgtgagttg ctggccgcac agacaggggc
    15961 taagatcgaa tatatttctt taggtggcgg tttcggcgtc gattacaagc agcttgatgg
    16021 tagcccgact tcttttgatt ttggtaccct gtcgc
//
LOCUS       DAOQJF010000023        15899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1749_length_15899_cov_4.771333, whole genome shotgun sequence.
ACCESSION   DAOQJF010000023 DAOQJF010000000
VERSION     DAOQJF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 15899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15899
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1749_length_15899_cov_4.771333"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="PLF85_02345"
     CDS             <1..1538
                     /locus_tag="PLF85_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE18525.1"
                     /translation="RLLKFQKELPKLEKIFVLESDDFQLAEGQDSVKQLIASGREMLS
                     QNPKLIENIAAVTNPAEMATIIYTSGTTGAPKGVMLGQSGWLNALDTALSMNVIHEKD
                     TALSLLPPWHAFERAVEYGVVMAQCPFMITGINSLRQDLADFKPTSFPSVPRIWESLY
                     NGIMQKLEKESPAKRALFHFFLDVGAEWAKNEAIFNGYDLQITPKPLPQQLADKAVAG
                     AKLAALLPLKLASGVVFGAIHKALGDNLKKSASGGSALPQVVDRFLGAIGVKVLEGYG
                     MTETSALISLRDIDRAVPGTVGRPFPGYKIRFRNEAGKDVPVQPGAKGTLWVHSNQLL
                     LGYYKRPELNEVVFDKDGFFDTGDVMVMTAGGDLMFAGRSKETLALAGGENIEPMPIE
                     DKLLASEYIDQVMVVGDDKKTLGALIVPNFEKVRSATGLKDLPTEKWNETTAIREIFK
                     KEIHERISNKTGFKNFELIPKNTFYIVPKQFEPGVELTRTYKLRRTVIKEIFAEQIEG
                     MYR"
     gene            complement(1535..3604)
                     /gene="amt"
                     /locus_tag="PLF85_02350"
     CDS             complement(1535..3604)
                     /gene="amt"
                     /locus_tag="PLF85_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNE18526.1"
                     /translation="MPEVKLTDQLWVLFASALVLFMQAGFLCVESGLTRTKNSINVAI
                     KNVTDFGVATVAFWLFGFGLMFGLSYSGIVGTSGFFTGFNGGSGWGSTFFIFQLMFCG
                     TASTIVSGAVAERMSFTAYLASTTIISLIIYPIAGHWAWGGALPETGKGWLANKGFVD
                     FAGSTVVHSVGGWVALAALLHIGARVGRFSEVDGSAQKMNPSNLPLAMLGGIILWFGW
                     IGFNGGSTLALNEQVGPVIAKTLLAGAVGMLAALFLGWWHDGYPEATQPLNGSLAGLV
                     SITAGCHAVNEWQAAVIGLVGGSLVLPVDKLLEKLRIDDAVGAIPVHLVGGIWGTLAV
                     GLFGAQAKLGTGLGRADQVLVQLVGILCIGAFAFSVAYLLLWILNRLHPLRVSPEDEK
                     TGLNISEHRARTDLIDLFFVMDHQKQTGDLSYNVPVEPFTEVGQIAERYNEVLARVRS
                     TLADNERAKSELVDAYRKVQHEQARAEQLLLNILPPKVADQLKDSNDIIANSFPEVSV
                     LFADIVDFTRLAHKFRPETIVGILNRVFSVFDELVEKYGLEKIKTIGDAYMVVGGLPN
                     PMANHAEAVANFALESMAQLKRFKLKTGDELTMRVGINTGPVVAGVIGKKKFIYDIWG
                     DAVNLASRMEANGLPGTIQISEHTAARLGGRFVLEERGEIDVKGRGRVKAFFLKGRRA
                     PAGLRPA"
     gene            complement(3738..4757)
                     /locus_tag="PLF85_02355"
     CDS             complement(3738..4757)
                     /locus_tag="PLF85_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE18527.1"
                     /translation="MLVLFQEGIPDIFFPALRQKGITVQRVPTGRTAAIAACCQYASA
                     EAIFFRANFTIDKELLEALPQLRLAALVSTGSDNVDVHALAERQIRFVSGEGANAQAV
                     CDYVLQALCFGGFNFARESLGVVGAGRVGSRLLKLLGSAGVRVSYFDPLLADPGSLEE
                     VLQCDVVTFHTFLSREGTFATAGMLDARYFAPVKKKLRLIQASRGGVWNTDFYRSLAS
                     HPHIEILAQDVYPGEPPRTSDLSLARYSTPHVAGYSTFGRLGGIAKGIQVLLTDFSAE
                     ALLPAGRAWFLDEEAQRFTDDPSAFNDLRDNYGWRKEFWQYNDEERAAWLTRFPQAPQ
                     SCFGW"
     gene            complement(4760..5464)
                     /locus_tag="PLF85_02360"
     CDS             complement(4760..5464)
                     /locus_tag="PLF85_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE18528.1"
                     /translation="MIRILDVHIDYQQGGALSVSKGGSRELVRALRGVTLDLNSNDYV
                     AIIGPSGSGKSTLVTAVAGLIAVAKGDVYVDKYKISRARRARQAKIRGKYFGIIFQFS
                     EMLGRFTVEENLRFAYVAAHGKIDDHYAERLQYLTQKLDMLPLLSTYPTRLSGGQLQK
                     TAIARALIKDSPFILADEPSGDLDPESVSRVKKLLREEHDRGKGIMLVTHDNKLASDA
                     KSVFEMRNGQISALIK"
     gene            5558..6172
                     /locus_tag="PLF85_02365"
     CDS             5558..6172
                     /locus_tag="PLF85_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18529.1"
                     /translation="MDIATLQNLEASGAHAAIEQNLAAAKTPQVLQYATAYYVRRGNP
                     AKVLTYYAEYVQATTAGERDLGAVAMAIECARRLGRHNLVTEYFLALNKQERERLSTE
                     ALTSVAAAFATLRQFAEAERIAGFIRARAGMPQLPDFDGYVKAKYGDSRAIRQFIENT
                     PPRFDRADTTGSVQKALSIAIAHMAEGNYPTAVAVLEQCKAVVS"
     gene            complement(6169..7674)
                     /gene="hemN"
                     /locus_tag="PLF85_02370"
     CDS             complement(6169..7674)
                     /gene="hemN"
                     /locus_tag="PLF85_02370"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNE18530.1"
                     /translation="MPGEEKTNRMLDNATVEALITRYNSPVPRYTSYPTALEFGPLAD
                     GVLLDSFRNIGRRPVGTQSDLVSRADLGHPCPPVSAQSDLVSRADLGHPCPSVSVYIH
                     LPFCENLCLYCGCNVLLSRKVEVLDEYIPLLLAEIKTKTDMMEFRPRVSQLHFGGGTP
                     NFLRLSDWDTLFAGLHTYFEFTPDAEISVELDPAVLTDGYLPHLKKLGFNRVSYGVQD
                     VTPKVLEAVNRPQDVGRIKAIVAESRALGFSSVNIDLIYGLPHQTNDSYKANLDFIGE
                     LRPERIALFSYAHVPWLKHHQKRMDAAALTEPSEKLRIYLSARTHFSALGYEQIGMDH
                     FGLPGDSLTEAYRNKTLHRNFMGYTTQKTLDMLAFGTSAIGFVSGVYMQNHLKLSAYR
                     NAIETGTNWFEKGFVMSTEDALRHEVITQLMANFRIRFAEIGQQFGISFKEHFAAEIE
                     RLREFAADGHGLLTDTDFTTTETGSFIIRHIASIFDAYRKGSEQKAFSKAI"
     gene            7790..10201
                     /gene="ppsA"
                     /locus_tag="PLF85_02375"
     CDS             7790..10201
                     /gene="ppsA"
                     /locus_tag="PLF85_02375"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNE18531.1"
                     /translation="MASTVTHKWILSFSEIRIEDVPLVGGKNASLGEMFRELKSKGVR
                     VPDGFAVTAEAYREFLRTTGLDRKIRELLKGLDANNAENLQQRGRAVREAILKEEIPE
                     GIRNEIFAAYDRLSEGRAGGIDVAVRSSATAEDLPNASFAGQQETFLNVHGEPQLLDA
                     CRRCFASLFTDRAISYREHQGFDHFQVALSIGVQRMVRSDLASSGVMFSIDTESGFKD
                     AVLINAAWGLGENVVQGAVNPDEFYVFKPTLAQGFKPILQKKTGSKEFKMVYDSGGGK
                     LVKNIPVNEAERSAFCIHEDDILTLARWAVTIQEHYTRKKGSVCPMDMEWAKDGITGE
                     LFIVQARPETVQSQRDTTKLEVYSLQEKGKVLIEGRSVGERIATGTVRVVKSAANLSL
                     VKQGDILVTDRTDPDWEPAMKKAAAIVTNRGGRTCHAAIISRELGLPAVVGTLDGTEK
                     LKNGEVVTVSCAEGDTGRVYAGQLKFSKETLNFSGNERPKTKVMINVANPEEAFRTSQ
                     VPNDGVGLARMEFIINSYIKIHPLALLNYDKLTDRAVIGQIDQLTKGYADKAQFFVDE
                     LARGVAMIGAAFYPHDVIVRMSDFKSNEYANLIGGAIYEPTEENPMIGFRGASRYYHE
                     KYRDGFALECRAMKKVREEMGLTNVKLMIPFCRTVDEGKKVLAEMEKHGLKRGENGLE
                     VYVMCEIPSNVILADEFSKIFDGFSIGSNDLTQLILGIDRDSEIVAPIFDERNPAVKT
                     MIANVIKAARANGRKIGICGQAPSDYPEFAEFLVREGIHSISLNPDAALKTMAHILKV
                     EKSLK"
     gene            10222..11364
                     /locus_tag="PLF85_02380"
     CDS             10222..11364
                     /locus_tag="PLF85_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNE18532.1"
                     /translation="MADRVIALAIFFLLAVVFLLAVKGRIVNRYLFTLARQFWLHHLV
                     SLGVLGLVLAHVAITFWRDWPDSASLYLNSADLPTIAGWAASGLLATVIASSWIAALG
                     WKRWYLLHLLSLPALVLAAYHGLSYASGRSVDMTVLLALLTLIAILLLRIFFERLGSQ
                     HSVRYEVVENRAVAAGIFELHLIATGTESTSLPAGNVVYLRFDSPGFSRAWHPFSVAS
                     STRDKDLRLMIKALGHDTGHISSLAAGSQVYVRGPFREFTPDVSRPQVWIAGGIGIAP
                     FIGYVSGLEQNSARRIELFYFTEKPSESIDLSPYVDQRRTSLVIHTQAHSGKPDLSAL
                     LTLAHELPQAQYTICGPPAFMSYTRQALLDAGITSSKIHTEEFQPW"
     gene            11358..11666
                     /locus_tag="PLF85_02385"
     CDS             11358..11666
                     /locus_tag="PLF85_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNE18533.1"
                     /translation="MVKHSIVFMFALLASAKAAEQTLRTTEISRHNKKSDCWISIDGS
                     VYDVTRYLERHKDFEYDIAKHCGTDTSKLWHKKPETGKPHSRKASRLLEKYRIGQPAQ
                     "
     gene            11722..12042
                     /locus_tag="PLF85_02390"
     CDS             11722..12042
                     /locus_tag="PLF85_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNE18534.1"
                     /translation="MDIASAKKNLETLRQQLLGRVSRIEDHIRRDGKALDQDSEEAVV
                     EKENDAVLDGLDAQGREELAQIDHALGRIASGQYQVCSKCGSGIGAERLQSLPYTTIC
                     VKCA"
     gene            complement(12058..12693)
                     /locus_tag="PLF85_02395"
     CDS             complement(12058..12693)
                     /locus_tag="PLF85_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HNE18535.1"
                     /translation="MSQRRFVLFLLLLIVVFSGGIFYALDATGIVRASDILPFLAPKN
                     PLRIEDKDYPTEVEKVEFQKWEQKLVEREEILARREAEIKSKESGSEEAKRELEEMRK
                     ALRAEKEKFAVAQKEWEDRQKKVKDLAEKVRNMPPQKAQEMMQSWRDFDIIDVMRQMD
                     KDAETEGTASIVPYLLTLFQPDRRAEITRKMMLPALTRPDAAEEEEPESAQ"
     gene            complement(12690..12935)
                     /locus_tag="PLF85_02400"
     CDS             complement(12690..12935)
                     /locus_tag="PLF85_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18536.1"
                     /translation="MNYPIKVKKAFTRVRYQVQDAWQNLRILKRHISCLVRSIDDVGS
                     GVSRSRQLLQGIAVREGGPCAASTAQADSVGHGGGYR"
     gene            complement(12937..13428)
                     /gene="fliJ"
                     /locus_tag="PLF85_02405"
     CDS             complement(12937..13428)
                     /gene="fliJ"
                     /locus_tag="PLF85_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNE18537.1"
                     /translation="MKKFRFHLESYLKLKKVREQQKLGELAKVMSRVNVFRAQQRAFE
                     DEYNHLLQQQREKFIRESLPIDSVREMYEYLGALRLRRDTATRNIAQLEGEVTEKRNA
                     YNAARKDRRVVEILKEKRLTEHRAATEREEIALMDEFNGARSRHNSLLWRDVSAEKKQ
                     EQV"
     gene            complement(13439..14845)
                     /locus_tag="PLF85_02410"
     CDS             complement(13439..14845)
                     /locus_tag="PLF85_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNE18538.1"
                     /translation="MIARNVSKEVRLLDKYRDIIRKTDTIRSLGSVLEIQGNVVLSRG
                     PEVRLGELCHIEVNSTIERFSPTGKRAEESYILAEVVGFRESTVILAPLEKIVGVSAG
                     SRVVSTGNMPAVSLTNEVLGRVIDGMGRPIDNRGMLVEGESRGLYGEPPQPLSRPLIR
                     TPLATGVKAIDAMLTLGQGQRVGIFAGSGVGKSTLLGMIAKYSTADVNVIALIGERGR
                     EVAEFIQEQLGESGRKKSVIVASIASESPMNRVRAGYLATTIAEYFRDKGKNVLLMMD
                     SVTRLAHAQREIGLSRGEPATTRGYPPSVFSMIPGLMERAGTSEKGSITGVYTVLVEG
                     DDMNEPIADTARGVLDGHIVLSRRLNSQNQYPAIDVAESISRTMPNVVEKDHLKLAQE
                     FREIYTAYKENEETINLGAYARGADPVIDNAILLREPMRKFLRQAPDEQIPFEETRRQ
                     LRDILGSLRARAAGPQRR"
     gene            complement(14858..15781)
                     /gene="fliH"
                     /locus_tag="PLF85_02415"
     CDS             complement(14858..15781)
                     /gene="fliH"
                     /locus_tag="PLF85_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNE18539.1"
                     /translation="MSRLVFNNAEIQKQNSAIVLELPDRYKKFQQLDELAEFELDAEG
                     NIVEQYTGPSIEEIEAELQRYREETEQEIKIRQKEAEKESQRITEEGKAEAFRLIQDA
                     KEKARKEEESARLQSSQIIERAKLEVERMVKEAELRVQDINHEAYQKGYNAGREQGYK
                     EGMGEVRRLIDRLGTILGNAIDVREQIIKESEKLMVEMILMIARKVIKDEIIERKEVV
                     LNNIREGLARIKERDRIDIRVNFADLELTTAHKDEIVKMMESLRKVNIYEDSRVDRGG
                     VIIETDVGAIDARISTQLKEIEQAIRDAKPM"
BASE COUNT         3527 a         4615 c         4311 g         3446 t
ORIGIN      
        1 agcgcctgct caaattccag aaagaactgc ccaagctcga aaagatcttt gttctcgaaa
       61 gcgacgattt tcagctagcg gaaggccagg actcggtgaa gcagctcatt gcctcgggac
      121 gcgaaatgct ttcgcagaac ccgaagctga tagaaaatat cgctgccgtc actaaccctg
      181 ccgaaatggc gacgatcatc tacaccagcg gaacaaccgg cgcgcccaaa ggggttatgc
      241 tcggccagag cggctggctt aatgcgctcg acacagcact cagcatgaat gtgatccatg
      301 aaaaagacac tgccctttcg ttgctgccgc cgtggcacgc atttgaacgc gcggtagaat
      361 acggcgtcgt gatggcacaa tgccctttca tgatcacggg catcaacagc ctgcggcagg
      421 accttgccga tttcaaaccc acgagttttc cctcggtacc ccgcatctgg gaatctctct
      481 acaacggtat catgcagaag ctcgagaaag aatctccggc caaacgcgcg ctgttccact
      541 tctttctcga cgtcggcgcc gagtgggcaa aaaatgaggc aatcttcaat ggctacgacc
      601 tgcaaattac tcctaagccg ctgccccagc agctcgccga caaggctgtc gccggcgcta
      661 aacttgccgc gttactgcca ctgaagctcg cctcgggcgt ggtctttggc gcgattcaca
      721 aggcactcgg cgacaatctc aaaaaatcgg cgtcgggcgg ctcggcgctg ccacaggtgg
      781 tcgaccgctt cctgggcgca atcggcgtca aggtcctcga gggttacggt atgaccgaaa
      841 cgtcagcgct gatcagcctg cgcgacattg accgcgcggt gccgggtacc gtcggccggc
      901 cgttccctgg ttacaagatc aggttcagga acgaagcggg caaagatgtc cctgtacaac
      961 cgggagccaa gggcacgttg tgggtgcact ccaaccagct gctgctcggc tattacaagc
     1021 gccctgagct gaacgaagtg gtcttcgata aagatggctt ctttgacacg ggcgatgtga
     1081 tggtgatgac tgcgggcggc gacctgatgt ttgccggccg cagcaaagaa acgctcgctc
     1141 tggccggcgg tgagaacatc gaaccgatgc cgatcgaaga caagctgctc gcgtcagagt
     1201 atatcgacca ggtcatggtt gtcggcgatg acaagaagac gctgggtgcg ctgattgttc
     1261 ccaacttcga aaaagtccgc tcagctaccg gcctcaagga cctgccgaca gaaaaatgga
     1321 atgagaccac cgcaatacgc gaaatcttca aaaaagaaat tcacgagcga atctcgaaca
     1381 agaccgggtt caagaacttc gagctgattc caaagaatac tttctatatc gtgccgaagc
     1441 agtttgaacc tggggtcgaa ctcaccagga cttacaagct ccgccgcacg gtcattaaag
     1501 agatttttgc cgagcagatt gaaggtatgt atcgctaggc agggcgcagc cctgcgggcg
     1561 cccggcgacc tttgagaaaa aaggccttca cccgacccct gcccttcaca tctatttcgc
     1621 cgcgttcttc aagaacgaag cgcccgccga gccgtgctgc cgtgtgttcg ctgatctgta
     1681 tggtacctgg cagaccgttc gcctccatgc ggctcgcgag gttgactgca tcgccccaga
     1741 tatcgtagat gaattttttc ttgccaataa cgccggcgac gacgggcccc gtgttgatgc
     1801 ctacccgcat ggtgagctcg tcgcccgtct tcagtttgaa tctctttagc tgagccatac
     1861 tttcgagggc aaaattggcc actgcttctg catggttcgc catcgggttt ggcagtcccc
     1921 ccacaaccat gtacgcatcg ccaattgttt tgattttttc aagaccgtat ttctctacga
     1981 gctcatcgaa aactgaaaaa acgcggttga gtataccaac aattgtttca ggcctgaact
     2041 tgtgtgccag ccgcgtaaaa tcgacgatat cggcaaaaag aaccgaaacc tcgggaaaac
     2101 tgttggcaat aatatcatta ctgtccttga gctggtcggc caccttaggc ggcagtatgt
     2161 tcagcagaag ttgctcagcg cgtgcctgct cgtgctgcac ctttcggtac gcatcgacca
     2221 gttcagactt ggcccgttcg ttgtcggcca gtgtgctgcg cacccgggcg agcacttcat
     2281 tataccgttc ggcaatctgc ccgacttcgg taaacggttc gactggtacg ttgtacgaaa
     2341 gatcgcctgt ctgcttctgg tgatccatca caaagaacag gtcaatcagg tcggtacgcg
     2401 cacggtgctc agaaatgttg agaccggttt tttcatcttc aggacttaca cgtaacgggt
     2461 gaagccgatt cagaatccag agcagcagat aggcaacgct gaaggcaaaa gccccaatac
     2521 aaagaatacc caccagttga accaacacct ggtctgcgcg gcccaagcct gtaccgagtt
     2581 tggcctgtgc gccaaaaagc ccgaccgcaa gcgtgcccca gataccgcca acaaggtgaa
     2641 ccggtattgc gccgactgcg tcgtcgatgc gcaatttttc gagtagtttg tcgacaggca
     2701 ggacgaggga acctccgacc agcccaatca ccgctgcctg ccattcattc accgcatgac
     2761 aacccgcggt gatggacacg agaccggcaa gcgaaccgtt cagcggttgc gtcgcttcgg
     2821 ggtacccgtc gtgccaccag ccgagaaaga gtgctgcgag catacccaca gcgcccgcca
     2881 ggagagtttt cgcaatgacc gggccgacct gttcattgag cgccagtgtg ctgccaccgt
     2941 taaagcctat ccagccgaac cagagtatga tgccccccaa catcgcgagc ggcaggttgc
     3001 tgggattcat tttctgtgcg ctgccatcga cttcgctgaa tctgccgacg cgcgcaccga
     3061 tgtgaagaag agccgcgagc gccacccagc cgccgacact atgcacgacg gttgaacccg
     3121 caaaatcgac gaacccctta ttcgccagcc agccctttcc cgtttcaggc agcgctccgc
     3181 cccatgccca gtgcccggct atcggataga taatgaggga gataattgtt gtgctggcaa
     3241 ggtaggccgt gaaactcatg cgttccgcca ccgcacctga aacaatcgtc gaggcagtac
     3301 cgcagaacat cagctgaaag ataaagaacg tcgagcccca accgctgccg ccgttgaagc
     3361 cggtgaaaaa gccactcgtg cccactatac ccgaatagct caggccgaac atcagcccaa
     3421 aaccgaacag ccagaaagcg actgtcgcta cgccaaagtc agtcacgttc ttgatagcga
     3481 cgttgatcga gtttttcgtc cgtgtcagcc ctgattcgac gcagagaaag cctgcctgca
     3541 tgaagagtac aagggcactg gcgaacagga cccacagctg gtccgtaagc ttgacttcgg
     3601 gcataagtaa ttgaattgtg agcaaattac atgcctgagc gaaccatgga agggttacgg
     3661 aatcagattg ggatagatgc taacgaaact tcagggaaaa tgctttgtgc gccgcgtaag
     3721 ccacgaagac actttactca ccaaccaaag cacgactgcg gtgcctgcgg aaacctcgtc
     3781 agccacgcgg cccgctcttc atcattgtac tgccagaact cttttcgcca gccgtagttg
     3841 tccctgaggt cgttaaatgc tgaagggtcg tcggtaaaac gctgcgcttc ttcgtcgaga
     3901 aaccaggctc ggccagcggg tagcagcgct tctgcagaga aatctgtcag cagtacctgg
     3961 attcctttcg cgataccgcc taaccggccg aaagttgaat aacccgcaac gtgcggcgta
     4021 gaataccgcg caagcgagag gtcactggtg cgtggtggtt ctcccgggta aacatcctgc
     4081 gcgagaattt cgatgtgcgg gtgcgaagcg agcgaacggt agaaatcggt attccacacc
     4141 ccgccgcggc tggcctgaat aaggcggagc ttcttcttta ctggcgcaaa atacctggcg
     4201 tcgagcatac ccgcagtggc aaatgtgcct tcacggctca ggaatgtatg aaacgtcacc
     4261 acgtcgcatt gcaggacctc ttcgagcgaa ccggggtctg ccagcagcgg atcaaaatac
     4321 gagacgcgca cacccgcgct gccaaggagc ttcaaaagtc gtgaaccgac acgccctgcc
     4381 ccgacaactc cgaggctttc gcgcgcaaaa ttgaatccgc caaagcatag cgcctgcaac
     4441 acatagtcgc agactgcctg tgcgttagcg ccttcaccac tcacgaaacg aatttggcgc
     4501 tctgccagcg catgcacatc gacgttgtca gagccggtcg agacgagcgc ggcgagccgg
     4561 agctgcggca gcgcttcgag taactcttta tcgattgtga agttcgcgcg gaaaaaaatc
     4621 gcctcggcgc tggcgtactg gcaacaggcg gcgatcgctg ccgtgcgacc ggtgggcaca
     4681 cgctgcacgg tgatgccttt ttgcctgagc gcgggaaaga agatgtcggg tatgccttcc
     4741 tggaaaagga ccagcatgcc tatttgatca gcgcactgat ctggccattg cgcatttcaa
     4801 aaaccgattt ggcgtctgaa gcgagtttgt tgtcgtgcgt gaccagcatg atgcccttgc
     4861 cgcggtcgtg ttcttcgcgc agcagcttct tcacccgcga gaccgattcg gggtcaagat
     4921 cacccgacgg ctcatcggcc aggataaaag gtgagtcttt gatcagcgcg cgcgcgatcg
     4981 cggttttctg caactggccg cccgaaagcc gcgtggggta agtggagagc agtggcagca
     5041 tgtcgagctt ctgcgtcagg tactgcaggc gttcggcata gtggtcatca atcttgccgt
     5101 gcgcggcaac gtaggcaaag cgcaggtttt cttcgaccgt gaaccggccg agcatctcag
     5161 agaactggaa gatgataccg aaatacttgc cacgtatctt cgcctggcgg gcccggcgtg
     5221 cgcgggaaat cttatacttg tcgacataga catcaccctt agccaccgcg ataagccccg
     5281 ccaccgccgt gacgagggta ctcttgccac tgcccgacgg accaatgatt gccacgtagt
     5341 cgtttgaatt gagatcaagg gtcactccgc gcagcgcgcg caccagctca cggctacccc
     5401 ccttcgacac gctcagggca ccgccctgct ggtaatcgat gtgcacatcg agaatgcgaa
     5461 tcatgtggag cgaaaatatt atgtcacatc cgggggtcaa taagtatccg gcgcctccgg
     5521 tggttgggac aattgcagga tgtgccgaaa gtatagtatg gacattgcta cgctgcaaaa
     5581 cctcgaagct tctggtgcgc atgccgcaat cgagcaaaat ctggcagccg ccaagacccc
     5641 gcaggtacta cagtacgcca ccgcctacta cgttcgccgg ggcaaccccg cgaaggtact
     5701 gacctactac gcagagtacg tgcaggccac cacggcggga gagcgagacc tcggagctgt
     5761 ggccatggcg atcgaatgcg caaggcgcct gggccgtcac aaccttgtca ccgagtactt
     5821 tttggcgctg aacaagcagg agcgcgagag gctgagcacc gaagcactga ccagcgtggc
     5881 tgcagcattt gccacgctac ggcagtttgc cgaagccgaa cggattgccg gctttattcg
     5941 ggctcgcgcc ggaatgccgc aactgccgga ctttgacggt tacgtcaaag caaaatacgg
     6001 cgactcacgc gccatcaggc agtttatcga aaacacaccg ccgcgctttg accgtgcaga
     6061 caccacgggc agcgtacaga aagccctctc gattgccatc gcgcacatgg ccgagggcaa
     6121 ctaccctacg gccgtcgcag tgctcgagca gtgcaaagct gttgtgagtt aaatcgcctt
     6181 gctgaaagcc ttctgctcgc tgcccttgcg gtaagcgtcg aagatcgagg cgatatgcct
     6241 gatgataaaa ctgcccgttt ctgtagtcgt gaaatcggta tctgtcagta agccgtgacc
     6301 gtctgccgcg aattcgcgca ggcgctcaat ctctgctgca aagtgctctt tgaaactgat
     6361 gccgaactgc tgcccgatct ccgcgaagcg gatacggaag ttggccatga gctgcgtaat
     6421 cacttcatga cgcagcgcat cttcggtgct catcacaaaa cctttctcga accagttcgt
     6481 accggtttcg attgcgttgc ggtaagccga aagtttcagg tggttctgca tatacacacc
     6541 ggaaacaaaa ccgatcgcgc ttgtaccgaa cgcgagcatg tcgagtgttt tctgcgtcgt
     6601 gtagcccatg aagttgcggt gcagcgtttt gttgcggtaa gcctcggtga gagaatcacc
     6661 tggcaggccg aagtggtcca taccgatctg ttcataaccc aaagcggaaa aatgcgtgcg
     6721 cgccgagagg taaatccgca gcttctctga aggttctgtc agcgctgcag catccatacg
     6781 tttttggtgg tgcttgagcc acggcacatg tgcataacta aagagcgcaa tacgctcggg
     6841 cctgagctcg ccgataaaat cgaggttggc cttgtagcta tcattcgtct ggtgcggcag
     6901 gccatagatc aggtcgatgt tgaccgaaga aaagcccaac gcccggcttt ctgcgacgat
     6961 tgctttgata cgcccaacat cctgcggccg gttgacggct tcgagcacct tgggcgtgac
     7021 atcctgcacg ccgtaactca cgcggttaaa gccaagtttt ttcaggtgag gcaggtaacc
     7081 gtcggtcaac actgccgggt cgagctcaac ggaaatttct gcatccggcg tgaattcaaa
     7141 gtacgtgtgc agtccggcaa agagtgtatc ccagtcggaa agccttagaa aattgggagt
     7201 tcccccgcca aagtgcaact ggctcaccct gggcctgaac tccatcatgt ctgtcttagt
     7261 cttgatctcg gcaagcagca gcggaatgta ctcgtcgagc acctcgacct tgcgcgacag
     7321 cagcacgttg cagccgcagt agaggcaaag gttttcgcag aatggcagat ggatatagac
     7381 tgaaaccgac gggcacggat gccccaggtc ggcgcgcgac acaaggtcgc tctgggcgct
     7441 gaccggagga cacggatgtc cgaggtcggc acgcgacacg aggtcactct gagtgccgac
     7501 aggacgtcgc ccaatgttgc ggaaactgtc gagtagcaca ccatcggcca ggggcccgaa
     7561 ttcgagcgct gtcgggtacg aggtataacg gggtaccggc gaattgtagc gcgtgatcag
     7621 cgcttcgacg gtggcattgt cgagcatacg gtttgttttc tcttcaccgg gcatgcgtca
     7681 acaacgggta gctgttgcgt cagcgatttg tggattatta ccgccttttg gctcgacacg
     7741 aaacgccgta cccgataaat ggatacacaa atcctttatt gggggccgca tggcaagtac
     7801 agttacacac aaatggattc tttctttcag cgaaattcgt attgaagacg taccactagt
     7861 gggcggtaaa aacgcctcgc tcggcgagat gttccgcgaa ctcaaatcaa agggagtgcg
     7921 cgtacctgac ggctttgcgg taaccgccga ggcctaccgc gaattcctgc gtacgaccgg
     7981 cctcgatagg aaaatccgcg agctcttgaa aggtctcgac gccaataacg cagaaaacct
     8041 gcagcagcgc ggccgtgcag tgcgcgaagc gatacttaaa gaagaaattc ccgaaggcat
     8101 ccgcaatgaa atcttcgctg cttacgaccg cctgtccgaa ggcagggctg gcggcatcga
     8161 tgtcgccgtg cgctcgagcg ctactgccga agacttaccc aacgcgagtt ttgccgggca
     8221 gcaggaaacc ttcctcaacg tgcacggcga accacaactg ctcgatgcct gccgtcgctg
     8281 cttcgcctcg ctcttcaccg accgcgccat atcgtaccgc gaacaccagg gcttcgacca
     8341 cttccaggtc gccctctcga tcggcgtaca gcgcatggtg cgctctgacc tcgccagttc
     8401 gggggtaatg ttttcgatcg ataccgaaag cggtttcaaa gatgccgtgc tgatcaacgc
     8461 cgcctgggga ctcggcgaga acgtcgtgca gggcgcagtc aaccccgacg agttttacgt
     8521 ctttaagccg acgctcgcgc aggggttcaa accaatactg cagaaaaaga ccggcagcaa
     8581 agaattcaag atggtctacg attccggggg cggcaaactg gtaaaaaata taccggtgaa
     8641 cgaggcggag cgttcggcgt tctgtatcca tgaagacgat attctgactc ttgcgcgctg
     8701 ggccgtgacg attcaagagc actatacccg gaaaaaaggc tcggtctgcc ccatggacat
     8761 ggaatgggcg aaggatggta tcaccggcga actcttcatc gtgcaggcac gtccggaaac
     8821 agtgcagtcg cagcgcgata ccaccaagct cgaggtctac agcctgcagg agaaaggtaa
     8881 ggttctcatc gaagggcgca gcgtcggtga gcgcatcgcc accggtacgg tgcgcgttgt
     8941 gaagtccgca gccaacctgt cgctggtcaa acagggcgac atactcgtta ccgaccgcac
     9001 cgaccccgac tgggagccgg cgatgaaaaa ggcggccgcg attgtgacca accgcggcgg
     9061 cagaacctgc cacgcagcga tcatcagccg agaacttggg cttccagctg tcgtcggtac
     9121 gctcgacggc acggaaaagc tcaaaaacgg tgaagtggtc accgtgagct gtgccgaagg
     9181 cgatacgggc cgtgtctatg caggccaact caaattcagc aaagagacac tcaacttttc
     9241 gggtaacgaa cgcccgaaaa ccaaagtgat gatcaacgtc gccaaccccg aagaggcgtt
     9301 ccgtaccagc caggtaccga acgacggcgt cggtcttgcg cgtatggaat ttatcatcaa
     9361 cagctatatc aagattcacc cgctcgcgct gctgaactat gataaactca ccgaccgcgc
     9421 ggtcatcggt cagatagacc agctgaccaa aggttatgcc gacaaggcgc agttctttgt
     9481 cgatgaactt gcgcgcggcg tggcgatgat cggtgcggct ttttatcccc atgacgtgat
     9541 tgtgcgcatg agcgatttta aatccaacga gtacgcgaac ctcatcggcg gggcaatcta
     9601 tgaacctacc gaagaaaacc cgatgatcgg ctttcgcggc gcttcgcgct actaccacga
     9661 aaaataccgc gacgggtttg cgctcgaatg ccgggcgatg aagaaggtgc gcgaagagat
     9721 gggacttacc aacgtgaagc tgatgattcc gttctgccgc acggtcgacg aaggcaagaa
     9781 ggtgctcgcc gaaatggaaa aacacggcct caaacgcggc gaaaacgggc tcgaggtcta
     9841 cgtgatgtgc gagattcctt ctaacgtgat tctcgcggat gagttctcga agatctttga
     9901 tggtttttcg attggctcca acgacctgac gcagcttata ctcggcatcg accgcgactc
     9961 agaaattgtc gccccgatct tcgacgagcg caaccctgca gtgaagacga tgattgccaa
    10021 cgtgatcaag gcggcgcgcg ccaacgggcg caagatcggt atctgcggcc aggcgccgag
    10081 tgactaccct gaattcgctg agttcctcgt gcgcgaaggt attcacagta tctcgctgaa
    10141 ccccgacgca gcgctcaaga cgatggcgca catactcaag gtcgagaaaa gcctgaagtg
    10201 acggcataga gtaggaaaac attggctgat cgcgtcatcg cactggcaat ttttttcctg
    10261 ctggcggttg tttttctgct ggccgtgaaa gggcgcattg tcaaccgcta cctgtttacc
    10321 ctggcacggc aattttggct gcaccacctg gtctcgctcg gcgtgctcgg cctggtactc
    10381 gcacacgtgg caataacttt ctggcgagac tggcccgatt cggcaagcct gtacctcaac
    10441 tccgctgacc tgcccaccat cgctggctgg gcagcttccg gtcttttggc tactgtcatc
    10501 gccagctctt ggattgccgc actgggctgg aaacgctggt acctgctgca cctgctctcg
    10561 ctgccagcgc tggtgcttgc agcctaccat gggctatctt atgcctcagg aaggtcagtc
    10621 gatatgaccg ttctcctggc gttacttacc ctgatcgcga ttttgctgct aaggattttt
    10681 ttcgaacgcc tgggcagcca gcactctgtc cgttacgagg ttgtcgaaaa ccgcgctgtc
    10741 gccgcaggaa tttttgaact gcacctcatc gccaccggaa cagagagtac ttcactgcct
    10801 gctggcaatg tcgtctacct gcgctttgac agtccgggtt ttagccgcgc ctggcaccct
    10861 ttctcggtgg cttcgagtac acgcgacaaa gatctgcgcc tgatgatcaa ggcgctcggt
    10921 cacgataccg gtcatattag ctccctcgcc gcgggcagcc aggtctacgt ccgtgggcca
    10981 tttcgcgagt tcacgccaga tgtttcgcgg ccacaggtgt ggattgccgg gggaatcggc
    11041 atcgctccct ttatcggcta tgtttcaggg ctcgaacaaa actccgcgcg gcgtattgaa
    11101 ctcttctact tcactgaaaa acccagcgag tcaattgacc tgtcgcctta cgtagaccag
    11161 cggcgcacaa gcctggttat ccatacgcag gcacattcgg gcaagcccga tctctcggca
    11221 ctactaacgc tcgcgcacga gctcccacaa gcccagtata ccatctgcgg cccgcccgct
    11281 ttcatgagtt acacgcgcca ggcgctgctc gatgcgggta ttacaagcag caagattcat
    11341 acggaggagt ttcaaccatg gtaaaacaca gcatagtatt catgttcgca ctgcttgcgt
    11401 cggccaaagc agcagaacag acgctcagga cgaccgaaat ttcacgtcac aataaaaaat
    11461 ctgattgctg gatttcaatc gatggctcag tctacgacgt gactcgctat cttgaacgcc
    11521 ataaagattt cgagtacgac atcgcgaagc actgcggaac cgacacaagc aaactctggc
    11581 acaaaaaacc cgaaaccggt aagccgcatt cgcgcaaggc gtcgcgcctg ctcgaaaaat
    11641 accgcatcgg ccagcccgcc caataaaaga catttttatc tttatttgtt tacacaacag
    11701 aagataattg cactgagcac tatggatatt gcatcagcaa aaaagaatct tgaaacactg
    11761 cggcagcaac tgctgggccg cgtcagtcgc atcgaagacc acatacgccg tgacggcaaa
    11821 gcactggacc aggacagtga agaagctgtg gtcgagaagg aaaacgacgc ggtgctcgac
    11881 ggactcgacg cgcaggggcg cgaagaactc gcacagatcg atcatgcact gggtcgcatc
    11941 gcctcaggac aataccaggt ctgcagtaag tgtgggtcag gcatcggtgc tgagcgcctg
    12001 caatcactgc cctacacaac tatttgcgtt aagtgcgcgt gaaaatcccg cgcccggtca
    12061 ctgggcgctt tctggttctt cttcttcggc cgcatccggc cgggtcagcg caggcaacat
    12121 cattttgcgt gtaatttcag cccgtcggtc gggctgaaac agggttaaca ggtacggcac
    12181 aatcgatgcc gtcccctctg tttcggcatc cttatccatc tgccgcatca cgtcgatgat
    12241 gtcgaagtca cgccagctct gcatcatttc ctgcgccttc tgcggcggca tgttgcgcac
    12301 cttttcggcc aggtctttca ccttcttctg ccggtcttcc cactctttct gcgctacagc
    12361 gaatttttct ttttctgccc gcagtgcctt gcgcatctct tcgagctcgc gtttggcttc
    12421 ttcgctgcca ctctctttgg acttgatctc tgcttcgcgc cgcgcgagta tttcttcgcg
    12481 ctcgacgagc ttctgctccc atttctggaa ctcgaccttt tctacctcgg tcgggtaatc
    12541 tttatcttcg atccgcaggg gatttttcgg cgcgaggaat ggcaatatat cagaggcgcg
    12601 gacaatacca gtagcatcga gagcataaaa gataccgccc gaaaagacta cgatcagcag
    12661 aagaaggaaa aggacaaatc gccgctggct catcggtaac ctccgccatg cccgacgcta
    12721 tcggcctggg cagtactcgc tgcgcacgga ccaccttcgc gcaccgcaat accctgcagc
    12781 agctgccggc tacggctcac cccgctaccg acgtcatcga tactgcgaac caggcagctg
    12841 atatggcgct tcaggatacg caggttttgc caggcatcct gtacctggta gcgaacccgg
    12901 gtaaaagctt ttttgacttt gatcggatag ttcatgttac acctgttcct gctttttttc
    12961 tgcgctcacg tcacgccaca gcaggctatt gtgccggctg cgcgcgccgt taaattcatc
    13021 cataagtgct atctcttcac gttcggtagc tgcacggtgc tccgtgagcc gcttttcctt
    13081 gaggatttca accacccggc ggtctttgcg ggccgcgtta taggcattac gcttttcagt
    13141 gacctcgcct tcaagctgtg caatgttgcg tgtcgccgta tcgcggcgca gtctcagagc
    13201 gcccaggtat tcatacattt cgcgcacact gtcgatcggc aacgactcgc gtataaattt
    13261 ctcgcgctgc tgttgcagca ggtggttata ctcatcttca aacgcccgct gctgtgcacg
    13321 gaacacgttg acccggctca tcactttggc cagttcaccg agcttctgct gctcgcgaac
    13381 tttcttcagc ttcagataac tctcgaggtg gaagcggaac ttcttcataa cctaggtttt
    13441 accttctctg cggccctgcc gccctcgccc gcagcgagcc gagtatatcc ctgagctggc
    13501 ggcgggtttc ttcaaacgga atctgctcgt cgggcgcctg gcgcaggaac ttgcgcatgg
    13561 gttcacgcaa cagaatggca ttatcgatca ccgggtcagc cccgcgcgcg tatgcaccga
    13621 ggttgatcgt ctcttcgttt tctttatagg ccgtataaat ctcgcggaat tcctgcgcga
    13681 gtttcaggtg gtctttttct accacgttcg gcatcgtccg gctgatcgat tcagccacgt
    13741 cgattgccgg gtactggttc tgcgaattga gccggcgcga aagtacaatg tgaccatcga
    13801 gcaccccgcg cgccgtgtcg gcgatgggtt cgttcatgtc gtcgccttcg acgagtacgg
    13861 tataaacgcc ggtgatagaa cctttctcgc tcgtacccgc gcgctccatc aggcccggga
    13921 tcatcgagaa taccgaaggc ggataaccgc gtgttgttgc cggttcgccg cgtgacaggc
    13981 cgatctcgcg ctgcgcgtgc gcgaggcgcg tcacagaatc catcatcaac aaaacattct
    14041 tgcccttgtc gcgaaaatat tcggcaattg tcgtggcgag gtaacccgcg cgcacgcggt
    14101 tcatcggcga ttcagaagca atcgatgcga cgatgacgga tttcttgcgc ccgctttcac
    14161 cgagctgctc ctggataaat tcggcaacct cgcggccccg ctcgccaatc agcgcaatca
    14221 cgttcacgtc tgcggtggaa tacttggcga tcatgcccag cagcgttgac ttacccacac
    14281 ccgaaccggc aaaaataccc acgcgctgcc cctgccccag cgtcagcatc gcatcgatcg
    14341 ccttgacgcc cgttgccagc ggcgtgcgaa tcagcgggcg cgagagtggc tgcggaggtt
    14401 caccgtaaag accacggctt tcgccttcga ccagcatacc gcggttgtca atcgggcggc
    14461 ccataccatc gatcacgcgg cccagcactt cattcgtgag cgatacagca ggcatattgc
    14521 ccgtcgaaac aacgcggctg cctgctgaaa ctcccacaat tttttcgagc ggcgcaagaa
    14581 tcaccgtgct ttcgcgaaag ccgactactt cggcgagaat gtaactttct tcggcgcgtt
    14641 tgccagtagg tgaaaaacgt tcgatggtgg agtttacctc gatatggcac aactcaccga
    14701 gacgcacctc tggtccgcgc gacagcacga cgttgccctg gatctcgagc acagaaccaa
    14761 gcgaacggat cgtgtccgtc ttccgtatta tgtctctata cttgtcaagg agccggactt
    14821 ctttgctgac gttgcgtgcg atcatctgcc tggttcctta cattggcttc gcgtcgcgaa
    14881 tagcctgttc gatttctttc agctgcgtcg agatacgtgc gtcgattgca ccgacatcgg
    14941 tttcgatgat cacgccgccg cggtcgacac gtgagtcttc gtagatattt accttgcgca
    15001 gcgattccat catcttgacg atttcatctt tgtgcgcagt ggtaagttcg aggtcagcga
    15061 agttaacccg gatatcgata cggtcgcgct ctttgatgcg agccaaacct tcacggatat
    15121 tgttgaggac aacctcttta cgctcgatga tttcgtcttt gataacttta cgcgcgatca
    15181 tcaggatcat ctcgaccatc agtttttctg attctttgat gatctgctcg cggacgtcga
    15241 tcgcattgcc cagaatcgta cccaggcggt cgatcaggcg gcgcacctcg cccatacctt
    15301 ctttatagcc ctgttcgcgg ccggcgttat agcccttttg gtaggcttcg tggttgatat
    15361 cctggacgcg gagctcggcc tctttgacca tgcgctcaac ttcgagtttg gcacgctcga
    15421 taatctggct cgactgcagc cgcgccgact cttcttcttt acgcgccttt tctttggcgt
    15481 cctggataag gcggaaagct tcggccttac cctcttctgt aatacgctgt gattctttct
    15541 ccgcctcttt ctggcggatc ttgatttcct gctcggtctc ttcgcggtaa cgctggagct
    15601 ccgcctcgat ctcttcgatc gacggaccgg tgtactgctc gacaatattg ccttcggcat
    15661 cgagctcgaa ttcggccagt tcgtcgagct gctggaattt cttataacgg tctggtaatt
    15721 ccagaacaat agcagaattc tgcttttgga tctcggcgtt gttgaatacc agacgtgaca
    15781 taatatgcct ttagaccacg cccccattaa ggtggagcga tccctgtact gcttttagta
    15841 tcggtgattg ggggatcgta ctgaagaact tgccctgctg gcagctccac cgctgaaaa
//
LOCUS       DAOQJF010000024        15704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1775_length_15704_cov_5.455365, whole genome shotgun sequence.
ACCESSION   DAOQJF010000024 DAOQJF010000000
VERSION     DAOQJF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1775_length_15704_cov_5.455365"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /gene="pheT"
                     /locus_tag="PLF85_02420"
     CDS             <1..2302
                     /gene="pheT"
                     /locus_tag="PLF85_02420"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNE18540.1"
                     /translation="IPVALIEELTKHPDADKLKICKVNTGKETVQIVTGAANVELGKK
                     FPAALVGVKLPDGREMTKAKLRGVESFGMLCSGGELGITALDYAPAKVDGLMPLPDDW
                     KVGAPLSDYFGAPDVILDIDNKSITHRPDLWCHFGFAREIAALLGKPLKKNAEDATFK
                     THPATALPKIEIEGRAAITYCGAELSGFKVQASPLARQLRLLSVGQKPINNIVDISNY
                     VLFEIGQPNHAFDREKISSLVKVSYSKSKEKIALLDGSTHELPEGLVVIRDGEKPAAL
                     AGVMGGAGSETTAQTAKLFLESATFHRSDIRAAVAKTGIRSDSSQRFEKAQNPGKAKA
                     AIYRFASLLAEEQASVTLSEIAEVSTEDYTKTQPIIVTNAFIDQKLGHIASRPDSNAA
                     ILKRLGFAVEDKGETLVVTPPAWRKWYDITIAEDLVEEIGRFIGYKEITIEPSLVACE
                     TPRAHNRLRELEHQLRDLCASRLQLNEVLSYAFQGEQDLKADEFFCSAGDAMKMKNSL
                     HADLGFMRTSPLPGILRALHGNYREHKNVRLFEIEKIFLPSKAANLARHESYFISGAL
                     TSEAADSDAAAQVAGMIGEILFTCGVLRHDQVFTNDAHGIFHPGRSGSAGTKDKKIFR
                     FGEIHPKLLAAFGIKDRVWYFDALLENLLGLKAEAPTYRPPFSHPAVDFDLTLVMPQR
                     AEFSALKAAAGVVQPLVRRDLDKTVMVSFDHVSTFTGGNLKEGEKAVSVRATWRNPVR
                     TLASDEIKKLQDGLIQKMQKAGFALR"
     gene            complement(2324..3445)
                     /gene="rffA"
                     /locus_tag="PLF85_02425"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(2324..3445)
                     /gene="rffA"
                     /locus_tag="PLF85_02425"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNE18541.1"
                     /translation="MNIPFNKTYLTGNEQKYLGATLQSGKFSGNGEFTRKCQQLLEQK
                     YSLRKTLLTTSCTDALEMAALLTGVGPGDEVILPSYTFVSTANAFALRGAKLVFCDSA
                     ADHPNIDAAAIEQLITPRTRVIVPVHYGGQACDMAHIMALAEKYKLWVVEDAAQAVDA
                     RAGEKYLGGIGHLGAFSFHETKNIIAGEGGALVINHEPFVKRAEIHWEKGTNRSAFFR
                     GEVDKYGWVDLGSSFLPSELNAAFLLAQLEAVEHIQGLRMKAWNTYHAALSRLGEKGV
                     RVMPNRPHNAHLFYLVCNSLDERTALIDYLKKQGIAAVFHYLSLHCSPYFAAKHDGRA
                     LPHSDRYTDCLVRLPMYAELGVDGAERVANEILQFFSSR"
     gene            complement(3442..4731)
                     /locus_tag="PLF85_02430"
     CDS             complement(3442..4731)
                     /locus_tag="PLF85_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18542.1"
                     /translation="MPRAQLKQNVSLKTGVRQKLHGTLTHAVQLLSLPQHELREEVLR
                     VIAENPFVEEISSGEAPPLSAGEGKDFIAKAHIGLQENLLLQLVDMGMPERIYDLARI
                     IVSTIDNDGFTTFPHRTIIGDYNFTSRDLRACIEYLKQLEPCGVGAENVWQSLRWQAE
                     VRYGKDELLFDLIDMLSNAREGLARFSQAEKKSLCEILEIDLETLEGKIRMLASLDPH
                     PVNRTDGTAAEWALPEVFFEVKEKQVYVKVSDQYLPRFRVNHKLYQQMKGTKNSEWQA
                     TYQTAKSLVETLQFRSDTLTQVARIIAVRQYDFFTKGSAYIKTMSLSDLARETKRHVS
                     TISRILNRKYFHCSWGIFPLRLFLMRRIKSSDGTERSAEDLKTAILTLLAQDKNRKQS
                     DRVIAEILQGQGFEVKRRTVNKYRRLIHQESSRKRQK"
     gene            complement(4735..5559)
                     /gene="lptB"
                     /locus_tag="PLF85_02435"
     CDS             complement(4735..5559)
                     /gene="lptB"
                     /locus_tag="PLF85_02435"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNE18543.1"
                     /translation="MPAEIKEKTGRKNSGATKEPAPIVGKPAPVIAPDALKLEAHNLV
                     KTYGKRTVVRGVSFNVRQGEVVGILGPNGAGKTTSFYMTIGLVKQSGGQVFLGGEDLT
                     NLPMYMRARKGIGYLAQEASIFRTLSVRKNIEAILELRGFSKADMAARADQLLKELEI
                     EHVQHQNGITLSGGERRRAEIARALASDPKFLLLDEPFAGVDPIAVKEIQRVVWQLQD
                     RGLGILITDHNVRETLKLTDRSYIIFQGQVLIEGTADVLIKDKKAREIYLGEDFKI"
     gene            complement(5560..6792)
                     /locus_tag="PLF85_02440"
     CDS             complement(5560..6792)
                     /locus_tag="PLF85_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HNE18544.1"
                     /translation="MAIEKEITLPCGATLRNRLAKSAMSENLALDGKPHEKLWHLYET
                     WGSGSLGLIITGNIMVDRNELGEPNNVILDSDDNLDHFREWTTRAKRQGAAIWAQLNH
                     PGRQAPAAISRTTVAPSAIKLKTGRLAFRTPHALTEPEIRSIIEKFANTARLCKAAHF
                     DGVQIHGAHGYLVSQFLSPLANERTDHWGGSIENRMRFVLEVYRAMRAAVGPKFPIGI
                     KINSADFQRGGFDESDATTVVKALEAEGIDMIEISGGTYESAVMTGVGGVRESTAERE
                     AYFLPFAAKIRLAIKIPIMLTGGFRSAHAMNAAIESGTVDIVGMARPLAMEPHLCVRL
                     LADTNARSEIQPVKTGIRFVDKLGVLELGYYGLQLARIGEGKHPDPNISPLLALPLIG
                     LNYSSGIIGRWLSGKAAD"
     gene            complement(6819..7553)
                     /locus_tag="PLF85_02445"
     CDS             complement(6819..7553)
                     /locus_tag="PLF85_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNE18545.1"
                     /translation="MAALLFLVLPAFAQADLQLLRGRFQPEKRQGFKAIPAKLSPGRV
                     QYLDERTLTAFEKMAAAAHKAGFQLTIVSATRNFTTQKAIWEQKFSGIRKVRGKDLTK
                     TIPNEELRALEILKYSSMPGTSRHHWGTDLDLHEAKLSGPALSNETLSKGRGLELYNW
                     LLAHAGEYGFCQPYKGNPNARNAGRFAHGYEEERWHWSYQPLSAEYLKVYTQQAGAMA
                     PGGFLGDKASGKFYLDYVKNIDPGCL"
     gene            complement(7599..7943)
                     /locus_tag="PLF85_02450"
     CDS             complement(7599..7943)
                     /locus_tag="PLF85_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE18546.1"
                     /translation="MDKVQLKDVAVATAIAYLHGRLDVAVSGEIEEHLLKSIDSKGIK
                     HLILNMADVEYMSSSGFRVMIALLRRLKDLKGSLKICCVRPAVKRIFDVIELTTLFDM
                     YETEEEALAAKA"
     gene            complement(8008..8628)
                     /locus_tag="PLF85_02455"
     CDS             complement(8008..8628)
                     /locus_tag="PLF85_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18547.1"
                     /translation="MPGRFVAIFLLLASAGRAQFFDMTAKGEKADLNRISVHAQVVRS
                     PIRIGLEYERTLFIWGWLKPAIGASANTGAQITEDTARGFRNSEYTSYIDAPVRLAVM
                     PIEYFQVYAGAGMTFYSTTLRLKDLGSLPGLEGSATKAGAAPFVEAGFRLSYGSYSVG
                     MSASYAQAADIAVRYSYISGGNKVFDSFQHTPFMNARASVFAGFLF"
     gene            8732..9037
                     /gene="clpS"
                     /locus_tag="PLF85_02460"
     CDS             8732..9037
                     /gene="clpS"
                     /locus_tag="PLF85_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNE18548.1"
                     /translation="MPAGVRNDEDSEVLTRAKKPKLYKVILLNDNYTTMEFVVYILQK
                     YFQKNEHEAKRIMLDVHNNGSGVAGIYPREVAETKVKQVMAHAEQEGFPLACKAEPE"
     gene            9066..11417
                     /locus_tag="PLF85_02465"
     CDS             9066..11417
                     /locus_tag="PLF85_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE18549.1"
                     /translation="MANTVEKIHQRAHDIARDFKNSIITFEHVLLAVTEDEEGAEILR
                     DLRVNIEELRRDLTQHIATFDQDLDADTELTESGALTNFREMLLVRMILNNNRELSIA
                     DVFDGIFRQRDSFAVYFLEKQGLSRAKVLRYLTHGITPAASPEEQAEMAEAGEENQAA
                     GESPDKILKAFTVNMTEEARAGKYDELIGRNDELNRTMEILCRRNKNNALHVGEPGVG
                     KTAIMRGLAQRLVKGNVPARLKGYEIFSIDVSSLVAGTRYRGDFEERMQAIIGALSAK
                     KKVILYIDEIHQIVGAGATRGDSMDAANMLKPVLTRGDMRCVGATTYEEYRRYFEKDR
                     ALSRRFQKVDISEPSREDAYQILLGLKKLYEEYHRVTYSNEILRKAVDLSAEHINERY
                     LPDKAIDVVDEAGASLHLYKPEQREVTEDDLRAIVSRLANVNLESFQGHESAGLKSIA
                     ENLKAKIFGQDAAIEKIARAIKRHRAGLGNKQKPIGSFLFTGPTGTGKTELTRAIASE
                     LGVELIRFDMSEYMEKHSVSRLLGSPPGYVGFDQGAQLTDAIRKHPRCVLLLDEIEKA
                     HPDVFNTLLQIMDYATVTDATGKKADFRHVLLVMTSNAGSGELNTARIGFEEKTKGAG
                     HVKSALKTFFTPEFLNRLDDVVVFDRLSDAVLDKIVDKNLAALNLQLAEKEITVALTA
                     AARRHLAEKGYDPVMGARPMARLFQDTLRDLVTDAIIDEKVSGGETVTMDFVDGQVVI
                     SDIGKLQASQGTAKSPVSQTAAAPAQKKKVARTEGAKPKKKKS"
     gene            11421..12689
                     /locus_tag="PLF85_02470"
     CDS             11421..12689
                     /locus_tag="PLF85_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE18550.1"
                     /translation="MGRVIVFKIVFAIITLTLLAFQYVIWKKIASRFTDDKTLRRLRV
                     VYWLFVGAGQSVMWFSVLYPGRGPATTLPEWYQPLHGVLLAINYSHFAWLLPLGFFWL
                     AGIVYSRINRTKSARPAVAEAGISRSEFLRSALGATAFGVNLLPAATSVTAISGIFLG
                     SREIAVMPKEIRLNGLHDDLKGLRLVQISDIHIGNLIGERYLNFTLGLIRDARPDYVV
                     VTGDIIDNNNVFLPVAANYFALIEAMLRRPTDKPGDSRLLGVLGNHDLIDNGLVAAQG
                     LSRAGLRILRNDVQMVRRGRGKLQIGGLDYPPLGRGRTEVMKAYFSEVKEKRSAETPL
                     VLLNHNPADFEFLKYQQVDLVLSGHTHGGQINFSRSQNTLLNGARWIYKYYVDHYAEQ
                     DSQLYVNRGLGHWFPLRMNCPPEITVITLA"
     gene            complement(12710..13630)
                     /locus_tag="PLF85_02475"
     CDS             complement(12710..13630)
                     /locus_tag="PLF85_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18551.1"
                     /translation="MSELKNEPVIAVKGRFRAPNQRYYGQFFYLINNKGGYIDVASVV
                     ITVGSDVYNALNPTDSWKKYESLINKLKYENKFSQAITNEFQAKLKQLALDKQYFADL
                     MKAVVGNKAETLNFVLANFYRRVIKDDEFYLEGHAEKASLDGNDETPASQEADIGGAD
                     GVYLPVKLDLDPISGKDVKEVRPGDKILVRVLPETERANAFIDGAGLRTDSGFVRSAP
                     FTVTSVIYPGVGVELVGKLKEGIYGKIVEEQNVLVRTAEAVKSAASPKAQAPLAASIP
                     DEKKQLMLIGIGVIGTALLGIVLYWVISSL"
     gene            13851..15020
                     /gene="metK"
                     /locus_tag="PLF85_02480"
     CDS             13851..15020
                     /gene="metK"
                     /locus_tag="PLF85_02480"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNE18552.1"
                     /translation="MNQERDFVFTSESVSEGHPDKVCDQISDAVLDAYLELDNTTRVA
                     CESLTTTDHVTVAGEISTSSKGHITKEKIEQIARDVMKDIGYVHDDIGFDYRTANVHI
                     LLHEQSQDIAQGVIGEGLHKEEGAGDQGMMFGFAIDETDALMPMPIYYSHKILEHLTG
                     LRKSGKLPFLRPDAKSQVTVRYEKGKPAGIQTVVVSTQHTPDVEHSKLSEQVIEECVK
                     KVLPAELLKGTNYYINPTGRFVVGGPHGDCGLTGRKIIVDTYGGYGRHGGGAFSGKDP
                     SKVDRSAAYMMRYVAKNVVASGIASQCEIQVAYAIGVAKPVSLVVNTFGTGKVGDDKI
                     EKMIMENFDLTPRGIRETLNLRNIKYRPTAAYGHFGREGQNSFTWEKTDKAGVFK"
     gene            15109..15387
                     /gene="rpsT"
                     /locus_tag="PLF85_02485"
     CDS             15109..15387
                     /gene="rpsT"
                     /locus_tag="PLF85_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNE18553.1"
                     /translation="MPNIASAKKRTRQNEKRRLKNQAQKSAIRTQEKKVRALVADKKV
                     DEAVKASQKLASLVDKASKTNLMHVNAASRKKSRIAQLVKKAAAAPAG"
     gene            15501..15574
                     /locus_tag="PLF85_02490"
     tRNA            15501..15574
                     /locus_tag="PLF85_02490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15535..15537,aa:Val,seq:tac)
BASE COUNT         3514 a         4319 c         4381 g         3490 t
ORIGIN      
        1 aattcctgtg gcgctgatcg aagaactgac aaaacacccc gacgctgaca aactcaaaat
       61 ctgcaaagtg aataccggca aagaaactgt gcagattgtg accggtgcgg cgaacgtcga
      121 acttggtaag aaatttcctg cggcgctcgt tggtgtgaaa ttgcctgatg ggcgcgaaat
      181 gaccaaggca aagctgcgtg gcgtcgagag ttttggtatg ctctgctcgg gtggcgaact
      241 cggtatcacc gcgctcgact acgctcctgc gaaagtagat ggcctcatgc cgctacccga
      301 cgactggaag gtgggtgctc cgctttctga ttatttcggc gcacccgatg tcattctcga
      361 tatcgataac aagtcgatca cgcaccgccc cgacctctgg tgccactttg gttttgcgcg
      421 cgagatcgcg gcgctgctgg gtaaaccgct gaagaagaac gccgaagatg cgacgttcaa
      481 gacgcatccg gccacggcac tgccgaaaat agagatagaa ggccgcgcag cgatcaccta
      541 ttgcggtgcg gagctcagtg gattcaaagt acaggcctca ccgctcgcgc ggcagctgcg
      601 cctgctgtcg gtgggccaaa agccgatcaa caacatcgtc gatatttcga actacgtact
      661 ctttgaaatt ggccagccga accacgcgtt cgaccgcgag aagatttcat cgctcgtgaa
      721 agtctcgtat tcaaagtcta aagaaaagat tgcgctgctc gatggttcga cacacgagct
      781 gcccgagggc ctggtggtta tccgcgacgg cgaaaaacca gcggcgcttg cgggagtgat
      841 gggtggggcg ggcagtgaga cgactgcgca gaccgcgaaa ctgtttctcg aatcagcgac
      901 tttccatcgc agcgacatac gtgctgcagt agccaaaacg ggaatccgca gcgattcgtc
      961 gcagcgcttc gagaaggcac aaaaccccgg caaggcgaag gccgcaatct accgctttgc
     1021 atcattgctt gcagaagagc aggcttcagt taccctctct gaaatagcag aagtttctac
     1081 cgaagactat accaaaacgc agccgatcat cgtcacgaac gcgttcatcg accagaaact
     1141 gggccacatc gcttcacgcc ccgacagcaa tgcggcgata ctgaaacgcc tggggttcgc
     1201 cgtagaagat aaaggcgaaa cgctggtggt cacgccaccc gcctggcgta aatggtatga
     1261 tattacgatc gccgaagacc tggtcgaaga aatcggccgc tttatcggct ataaagaaat
     1321 cacgatcgaa ccatcgctgg tcgcctgcga gactccccgc gcgcacaaca ggttgcgcga
     1381 gctcgaacac cagctgcgcg acctctgcgc ttcacgcctg cagctgaacg aagttctgtc
     1441 gtatgccttc cagggcgagc aggatctcaa ggccgatgag ttcttctgca gcgccggtga
     1501 cgcaatgaag atgaagaatt cactgcacgc cgatctcggc ttcatgcgca cgtcgccgtt
     1561 gccgggcata ttgcgcgcgc tgcacggcaa ctaccgcgag cataagaatg tgcggttgtt
     1621 cgaaattgaa aagatcttcc tgccttctaa agctgccaat ctggcgcgcc acgagagtta
     1681 ttttatctca ggtgccctca cgtcagaagc tgccgacagc gacgccgccg cgcaggttgc
     1741 ggggatgatc ggcgagatac ttttcacctg cggcgtgctg cgccatgacc aggtcttcac
     1801 caacgatgcg cacggcattt ttcacccggg acgctcaggc agtgccggga ccaaagacaa
     1861 gaagatcttc cgcttcggtg aaattcaccc aaaacttctg gcggcctttg gtatcaaaga
     1921 ccgagtctgg tacttcgatg ccctgctcga gaacctgctc ggtctcaaag cagaagctcc
     1981 gacctaccgg ccgccattca gccacccggc tgttgatttc gacctgacgc tcgtgatgcc
     2041 gcagcgcgct gaattctctg cgctcaaggc agctgcgggt gtcgtgcaac cgctggtgcg
     2101 ccgcgacctc gataagactg tgatggtcag cttcgaccat gtgagtactt ttaccggcgg
     2161 caacctgaaa gaaggtgaaa aggcagtctc tgtgcgcgct acctggcgca accctgtacg
     2221 cacgctcgct tcagacgaga tcaagaagct acaggatggt ctgatccaga aaatgcagaa
     2281 agccggtttc gcgctgcgct aagtcccgtt aacccctctg gggttaacgg gaggaaaaaa
     2341 actgcagaat ttcgttcgcg acgcgttcag cgccgtcaac cccaagctcc gcgtacatcg
     2401 gtagcctgac caggcagtcg gtgtaacgat cggaatgcgg cagtgcacgt ccgtcgtgtt
     2461 ttgccgcaaa gtaagggctg cagtgcagcg agaggtagtg aaagactgca gcaataccct
     2521 gtttcttcag gtagtcaatc agcgcagtgc gttcatcgag cgagttgcag acgaggtaga
     2581 aaaggtgtgc attgtgcggc ctgtttggca taacgcgcac acctttttct ccgaggcgcg
     2641 aaagagcagc gtggtaggtg ttccaggcct tcatgcgtaa accctggatg tgttcaaccg
     2701 cttcgagttg cgccagcagg aacgcggcat tcagctctga cggcagaaac gacgagccaa
     2761 ggtcaaccca gccgtactta tcgacttcgc cccggaaaaa agcgctgcgg ttggtgcctt
     2821 tttcccagtg gatctctgca cgtttcacga acggctcatg gttaatgacg agtgcaccgc
     2881 cttcgccggc gatgatattt tttgtctcgt gaaaagaaaa agctcccagg tgaccgattc
     2941 cgcccaggta tttttctccc gcgcgcgcat ccactgcctg cgcggcatct tcgacgaccc
     3001 agagcttgta cttttccgca agcgccatga tgtgcgccat gtcgcaggcc tgcccgccat
     3061 agtggactgg aacaataacc cgtgtacggg gagttatcag ttgttcaatt gcagcagcgt
     3121 cgatgttcgg gtggtctgcg gcactatcgc agaagaccag cttcgcgccg cgcaacgcga
     3181 aggcattggc ggtggagaca aaggtgtatg atggcagtat cacctcgtca ccggggccga
     3241 cacccgtcag cagcgccgcc atttcgagcg cgtcggtaca cgaagttgtc agtaacgtct
     3301 tgcgcaggga atacttctgt tcgagcagct gctggcattt ccgggtaaac tcgccattgc
     3361 cggaaaattt tccagactgc agtgtcgctc ccaggtattt ctgttcgttg cctgtcaggt
     3421 aggttttgtt aaaggggata ttcatttttg tctcttgcgc gaagattcct ggtgtattaa
     3481 tcgccggtat ttgttgacgg tgcggcgttt gacttcaaag ccctggccct gcagaatttc
     3541 tgcaatgacg cggtctgact gcttgcgatt cttatcctgc gcgagcagcg tgagaatcgc
     3601 tgttttcagg tcttcagcac tccgctcggt gccgtcagaa gacttgatac gccgcatgag
     3661 aaacagccgt agcggaaaaa ttccccacga acagtgaaag tatttgcggt tcagtatgcg
     3721 gctgattgtc gaaacgtggc gtttggtttc ccgtgcgagg tcgctgaggc tcatcgtctt
     3781 gatatatgcc gagcctttgg taaaaaaatc gtactggcgg actgctatga tacgggccac
     3841 ctgcgtcagg gtgtctgaac ggaactgcag cgtttcgacg agtgattttg ccgtctggta
     3901 ggttgcctgc cattcgctgt tctttgtacc cttcatttgc tggtacagct tgtggttcac
     3961 acgaaagcgg ggcaggtact ggtcagagac cttgacgtag acctgctttt ccttaacttc
     4021 aaagaatact tcaggcaatg cccattctgc ggcagttcca tcggtgcggt ttaccgggtg
     4081 cggatcaaga ctcgccagca tccgaatttt gccttcgagt gtttccaggt cgatttcgag
     4141 gatttcgcac agcgatttct tttcggcctg cgagaaccgg gcgagtccct cgcgcgcgtt
     4201 gctgagcatg tcgatcaggt cgaagaggag ctcgtctttg ccataccgta cctcggcctg
     4261 ccagcgcagg ctttgccaga cgttctcggc gccgaccccg cagggttcga gttgtttaag
     4321 gtattcgatg caggcacgca ggtcgcggct ggtgaagttg taatcaccga tgatggtgcg
     4381 atggggaaac gtggtgaaac cgtcattgtc gattgtcgat acgatgatgc gggcgagatc
     4441 atagatgcgc tcgggcatgc ccatatcgac cagctgcagc agcaggtttt cctgcaggcc
     4501 gatgtgcgct ttggcgatga aatctttgcc ttctccagct gagaggggcg gagcctcacc
     4561 cgacgaaatt tcttcaacga acgggttctc ggcgattacc ctgagtactt cttcgcgtag
     4621 ttcatgctgc ggtagcgaca gcagttgaac cgcatgcgta agggtgccat gcagcttttg
     4681 ccggacgccg gtcttgagag agacgttctg cttgagctgt gccctgggca taggctaaat
     4741 cttaaaatct tcgccgaggt agatttcgcg tgctttctta tctttaataa gcacatccgc
     4801 cgtaccctcg atcagcacct gtccctggaa gataatgtat gaccggtcgg tgagcttcag
     4861 cgtttcgcgg acgttgtggt cggtaatcag aattcccaga ccgcgatcct gcagctgcca
     4921 gacaacccgc tgtatctctt tcaccgcgat ggggtcaacc cctgcaaaag gttcgtcgag
     4981 cagcaagaat ttcgggtcag aggcgagtgc gcgcgcgatc tcggcacgcc tgcgctcccc
     5041 gcccgaaagg gtaatgccat tctggtgctg cacgtgttct atttcgagtt ctttgagcag
     5101 ctggtcggcg cgcgccgcca tatctgcctt gctgaagccg cgcaactcga gaatcgcttc
     5161 aatattcttg cgcacgctca gtgtccgaaa aatcgaggct tcctgcgcga ggtagccgat
     5221 gcccttgcgc gcacgcatgt acatcggcag gtttgtcagg tcttcgccgc ccaggaaaac
     5281 ctgtccaccc gattgtttca cgaggccgat cgtcatgtaa aaagaagtgg tttttcccgc
     5341 accgttgggg ccgagaatgc cgaccacttc gccctggcgg acattgaacg acaccccgcg
     5401 caccaccgtg cgcttgccgt aggttttcac caggttgtgc gcctcgagtt tgagtgcgtc
     5461 cggagcgatc accggtgcgg gtttgccgac gatgggggcc ggttctttcg ttgcaccgct
     5521 gtttttacgg ccagtctttt cttttatctc tgcgggcatt tagtctgcag ccttacccga
     5581 aagccagcgg ccgattatgc cagaagaata gttcaggcca atgagtggta atgccagcag
     5641 ggggctgata ttcggatcag gatgtttacc ttcgccaata cgcgccagtt gcaggccgta
     5701 gtaacccagc tccagcacac ctaacttgtc gacgaaacgg atacccgttt tgacgggctg
     5761 tatttcgctg cgtgcattcg tgtctgcgag cagacgcacg cagaggtgcg gctccatcgc
     5821 cagcgggcgt gccatgccga cgatgtcgac tgtacccgat tcgatcgccg cattcattgc
     5881 gtgggcactg cggaaaccgc cggtgagcat gatggggatt tttatcgcga ggcgtatttt
     5941 tgccgcgaac ggcaaaaaat acgcctcgcg ctcagcggtc gattctctta caccaccgac
     6001 ccctgtcatg acggcactct cataggtacc gccggaaatt tcgatcatgt cgatgccttc
     6061 ggcttcgagt gctttcacaa ccgtcgtcgc gtcactttcg tcgaaacctc cgcgctgaaa
     6121 gtcggcggag ttgatcttga taccaatcgg gaatttgggc ccaaccgccg cacgcatggc
     6181 acggtaaacc tcgagcacga agcgcatgcg gttctcgatg ctgccacccc aatggtcggt
     6241 gcgctcgttg gcgagcggag acaagaactg actcacgagg tagccgtgcg cgccatgaat
     6301 ctgcactccg tcaaagtgtg ctgctttgca gagtcgggcc gtattggcaa atttttcaat
     6361 aatgctgcgg atttccggtt cggtcagcgc atgcggcgta cgaaaggcaa ggcgtcctgt
     6421 tttcagttta atggccgagg gggcgacggt cgttctggaa attgctgcgg gcgcctgccg
     6481 gccgggatgg ttaagttgcg cccagattgc tgcaccctgg cgtttggcgc gggtcgtcca
     6541 ttcgcgaaaa tgatcgagat tgtcatcgct gtcgagtatc acgttattgg gctcgccgag
     6601 ttcattgcgg tcaaccatga tgttgccggt aataatgagg ccgaggctgc ccgaccccca
     6661 tgtttcgtaa agatgccaaa gcttttcgtg gggcttgccg tcgagcgcaa ggttctcgct
     6721 catcgctgac tttgccaggc gattcctgag cgttgccccg cagggcagcg tgatttcttt
     6781 ttcaatcgcc atagtacctc gtctgctgac gggctttgtc agagacagcc ggggtcaata
     6841 ttctttacgt aatcgaggta aaacttcccg ctcgctttat cgccgagaaa cccgccgggg
     6901 gccatggccc ccgcctgctg cgtgtacacc ttcaggtatt cagcagaaag gggctggtat
     6961 gaccaatgcc agcgttcttc ttcataaccg tgcgcaaaac gccctgcgtt gcgcgcattg
     7021 ggatttccct tgtaaggctg gcagaagccg tattcgccgg cgtgtgccag cagccagtta
     7081 tacagttcaa gcccgcgtcc cttcgacaac gtctcgttgc tgagggcagg cccggaaagt
     7141 ttggcctcat gcaggtcgag atcggtaccc cagtggtggc gagacgtacc gggcatcgag
     7201 gaatatttaa gtatttcgag tgcacgtagt tcttcgttgg ggatcgtctt ggtgaggtct
     7261 ttaccccgca ccttgcgtat gcccgaaaac ttttgctccc agatagcttt ctgcgtggta
     7321 aaattgcgcg tcgccgagac aatggttagc tgaaaaccgg ccttgtgtgc cgctgcggcc
     7381 attttttcaa aagcggtaag agtgcgctca tcgagatact ggacacgtcc cggggaaagt
     7441 ttagcgggga tggctttaaa tccctgcctt ttttccggtt gaaacctgcc ccgcagcagc
     7501 tgcaggtcgg cttgcgcgaa cgcaggtaac accagaaata ataatgcagc catgcgcacg
     7561 cgacgcgacg caatggtttt gcccggagtt ttcagaactt atgccttggc cgcgagggct
     7621 tcttcttccg tttcgtacat gtcgaacagg gtagtcagtt caatgacatc aaaaatccgc
     7681 ttcactgcgg ggcgtacgca gcaaatcttg agggagccct tgagatcttt gagccttctc
     7741 agcagcgcaa tcatcacgcg aaaacccgac gaagacatgt attcgacgtc tgccatgttg
     7801 agaatgaggt gtttgatgcc cttggagtcg atgcttttca gcaggtgttc ttcgatttca
     7861 ccggagaccg ctacgtcaag acggccatgc aggtaggcga ttgccgttgc cacggccacg
     7921 tctttaagct gcaccttgtc cattgccgcc agtatccaaa ggaaagttgg cccttcaaca
     7981 tatttttggc ggccccgcag ggttgggtca gaataagaat ccggcaaata cgctcgcccg
     8041 cgcattcatg aacggagtgt gctgaaagct gtcaaaaacc ttgtttccgc ccgaaatgta
     8101 gctgtagcgg actgctatgt ctgccgcctg tgcgtagctg gcgctcatgc ccacagaata
     8161 tgaaccgtag ctgagccgaa agcctgcttc gacaaaggga gctgccccag ccttggtcgc
     8221 agaaccctcg agaccaggta agctccccag gtctttcagg cgcagcgtcg tggagtagaa
     8281 ggtcatgcct gcgccggcat acacctggaa gtattcaatc ggcatgacag ccagccgcac
     8341 cggtgcgtcg atatacgaag tgtactcact gttgcgaaag ccgcgtgccg tgtcttcagt
     8401 tatctgcgct ccggtattgg ccgatgcgcc gatcgcgggc ttgagccagc cccagataaa
     8461 gagcgtccgt tcgtattcca gcccgatgcg tatcggtgag cggactacct gtgcatgaac
     8521 ggagattcgg ttgaggtcgg ccttttcgcc ctttgcggtc atgtcgaaga attgcgcccg
     8581 gccggcgctg gccaggagca agaagatggc aacgaacctg ccgggcatgt agcccgcacc
     8641 accgcttttt cgctgccgcc cgaaaagcag aatttgtccc cgcccggcaa tttttggttc
     8701 acgatttaat ccttagtata tttgcgggta tatgccagcc ggcgtgcgca atgatgaaga
     8761 ctccgaagta ctgacgcggg ccaaaaagcc taagctctac aaggttatac tcctgaacga
     8821 caattatacc accatggaat ttgtcgtgta tattctgcag aaatacttcc agaaaaatga
     8881 gcacgaagcc aagcgtatca tgctagacgt gcacaacaac ggcagcggtg ttgcgggcat
     8941 ttacccgcgc gaagtcgccg agaccaaggt gaaacaggtg atggcgcatg cagaacaaga
     9001 gggatttccc ctcgcctgca aggcagaacc ggaatagcgg ccaggacccg ccaggaaaca
     9061 agactatggc aaacacagta gagaagatcc accagcgggc gcacgatatt gcgcgcgatt
     9121 ttaagaactc gatcattacc ttcgagcatg tcctgctggc cgttaccgaa gacgaagagg
     9181 gtgcagagat cctgcgcgac ctgcgtgtca atatcgaaga actccgtcgc gacctgacgc
     9241 agcatattgc taccttcgac caggacctcg acgctgatac cgaactgaca gaatcaggcg
     9301 cattgacaaa ttttcgcgaa atgctgctcg tgcgcatgat acttaacaat aaccgcgagc
     9361 tgtcgatcgc tgacgtcttt gacggtattt ttcgccagcg cgactcgttt gccgtgtact
     9421 ttctggaaaa gcagggcctg agccgcgcaa aagtattgcg ttacctgacg catggtatta
     9481 cgcccgcggc atcgcccgaa gagcaggcag aaatggccga ggctggcgaa gaaaaccagg
     9541 ctgcgggtga aagtccggac aagatactca aggcatttac cgtcaacatg accgaagagg
     9601 ccagagccgg aaagtacgac gagcttattg gccgcaacga cgaactcaac cgcacgatgg
     9661 agattctctg tcgccgtaac aagaataacg cgctgcacgt cggtgaaccg ggtgttggca
     9721 agacggcaat catgcgcggg ctggcgcaga ggttggtgaa gggtaacgtg cccgcacgtc
     9781 tcaagggtta cgaaattttc tcgatcgatg tatcgagcct cgttgccggc acacgttacc
     9841 gcggcgactt tgaagagcgc atgcaggcga tcatcggtgc gctcagcgcg aagaagaaag
     9901 tcatactcta tatcgacgag attcaccaga tcgtcggcgc aggtgcaacc cgcggcgaca
     9961 gcatggacgc agcgaacatg ctcaagcccg tgctgacgcg cggcgacatg cgctgcgtcg
    10021 gtgcaacgac gtacgaagaa taccgtcgct atttcgaaaa agaccgcgcc ctctcacgcc
    10081 ggttccagaa ggtggatatc tccgaacctt cgcgcgaaga tgcctaccag atactgctcg
    10141 gcctgaagaa actctacgaa gagtaccacc gcgtcactta cagcaacgaa attctgcgta
    10201 aggccgttga cctgtcggcc gagcacatca acgaacgata cctgcctgat aaggcgatcg
    10261 acgtggtcga cgaagcggga gcttcgttgc atctctacaa acccgagcag cgcgaggtta
    10321 ccgaagacga tctgcgtgca atcgtttccc ggttggcaaa tgtcaacctg gagtcattcc
    10381 agggacatga aagtgcaggc ctcaagtcga tcgccgaaaa tctcaaggcg aaaatttttg
    10441 gccaggatgc cgcaatcgaa aagattgcgc gggcgatcaa gcgccaccgc gccggcctgg
    10501 gcaacaaaca gaagccgatt ggcagtttcc ttttcaccgg gccaacgggt acaggtaaaa
    10561 ccgagctcac ccgcgcgatt gccagcgaac tcggcgttga acttatccgt ttcgacatga
    10621 gtgaatacat ggaaaagcac agcgtctcgc gcctgcttgg atcgccacct ggttacgtcg
    10681 gtttcgacca gggtgcacag ctgaccgatg caatacgcaa gcatccacgc tgtgtgctac
    10741 tgctcgatga gatcgagaaa gcgcatcccg atgtgtttaa tacgctattg cagattatgg
    10801 actatgccac cgtcaccgac gccacgggca aaaaagcgga tttccggcat gtgctgctcg
    10861 tcatgacctc gaatgcgggt tcgggggagc tcaacacggc acgtatcggc ttcgaggaga
    10921 aaaccaaggg cgcaggtcat gtcaaatcgg cgctcaagac attctttacg ccggaatttc
    10981 tgaaccgtct cgatgacgtg gttgtgtttg acaggctttc tgatgcggtg ctcgacaaga
    11041 ttgtcgataa gaacctcgcc gcactcaacc tgcagctggc tgaaaaagaa attactgtag
    11101 cgctcactgc cgctgcacgc cgtcatctcg cagaaaaagg ctacgacccg gtcatgggcg
    11161 cgcgcccgat ggcacgcctt ttccaggata cgctgcgcga tcttgtgact gacgcgatta
    11221 tcgatgaaaa ggtcagcggt ggcgaaaccg ttactatgga ctttgtcgat ggacaggttg
    11281 tcatcagcga tatcggtaag ctacaggctt cccaagggac agcgaagtcc ccggtatccc
    11341 aaactgcggc tgcacctgcg caaaagaaga aggttgcccg caccgaaggc gcgaaaccaa
    11401 aaaagaagaa gtcctgaccg atgggccggg tcattgtctt taagatcgtt tttgcgatca
    11461 tcacgctgac gctactggcc ttccagtacg ttatctggaa aaaaatcgcg tcacgtttta
    11521 ctgatgacaa gaccttaagg cgactgcgtg tcgtttactg gctgttcgtc ggcgccgggc
    11581 agtcagtcat gtggttctct gtgctttacc caggccgggg gcctgcaacg acgctgcctg
    11641 aatggtacca gccgctgcac ggcgtgctgc tcgcgatcaa ctattcgcat tttgcttggc
    11701 tgctccccct ggggtttttc tggctggcgg gcatcgtcta tagccggatc aaccggacaa
    11761 agagcgcccg gccagctgtg gcagaagcag ggatttcacg ttccgagttt ttgcggagtg
    11821 cgctgggtgc aacagccttc ggcgtgaacc tgttgcctgc agcaacgagt gtcacagcga
    11881 tcagcggcat tttcctggga tcaagggaaa ttgccgtgat gccgaaggaa atccgcctga
    11941 acggtctgca cgatgacctg aagggattgc gcctcgtaca gatttcagat attcacattg
    12001 gtaacctgat cggcgagcgt tacctgaatt ttactcttgg tcttatacgc gatgcgcgcc
    12061 cggattatgt tgtcgtgacg ggtgatatca tcgacaacaa caacgtgttc ctgcccgtcg
    12121 cggccaacta cttcgcgctg atcgaagcca tgctgaggcg acccaccgac aagcccggtg
    12181 acagccgcct gctcggcgtg ctcggtaacc acgacctgat cgacaatggc cttgtggcag
    12241 cgcaggggct cagccgcgcc gggttgcgca ttctgcgcaa cgatgtgcag atggtgcgcc
    12301 gtggacgcgg caagctgcag attggcggtc ttgattatcc tccgctcggg cgtggccgaa
    12361 cagaagtgat gaaggcatat ttctcagagg taaaagagaa acgcagtgct gaaactcccc
    12421 tcgttttgct taaccataac ccggcggatt ttgagtttct taaataccag caggttgacc
    12481 tcgtgttgtc ggggcatacg cacggggggc agatcaattt ttcgcgctcg cagaatacgc
    12541 tgctgaacgg cgcccgctgg atatacaagt attatgtgga ccactacgcg gaacaggatt
    12601 cacagctgta tgtcaaccgg ggcctcggcc actggtttcc gctgcgcatg aattgcccgc
    12661 cggaaattac ggtgattact ctcgcctgac gaccggggtt ttacttttct tacagcgacg
    12721 aaatcaccca gtagagcacg atacccagaa gggcagtgcc gataacaccg atgccaatga
    12781 gcatcagctg tttcttctca tcggggatag aagccgccag tggagcctgt gccttgggtg
    12841 acgccgctga ctttaccgct tctgccgtcc gcaccagtac gttttgttct tcgacgattt
    12901 tgccatagat gccctctttc agtttaccga cgagctcgac acccacgccg gggtagatca
    12961 cgcttgtcac ggtaaaaggc gccgagcgga caaaaccact gtcagtcctg aggcctgcac
    13021 cgtctatgaa tgcattcgca cgctctgttt ctggcagtac acgcacgagt atcttgtcgc
    13081 ccggccgcac ttctttgaca tccttgcccg aaatcgggtc gagatcaagc tttaccggca
    13141 ggtagactcc gtcagccccg ccgatgtcgg cttcctggct cgccggcgtt tcatcgtttc
    13201 cgtcaagcga tgccttttct gcatgccctt cgaggtagaa ctcgtcgtct ttgatcacgc
    13261 gccggtagaa gtttgcgaga acgaaattca gcgtctcggc cttgttgccg accacggcct
    13321 tcatcaggtc agcgaagtac tgtttatcga gggccagttg tttcagtttc gcctggaatt
    13381 cattcgttat cgcctgcgag aatttgttct cgtattttag tttattgatg agcgactcat
    13441 atttcttcca tgagtcggtg ggattcagtg cgttatagac gtctgagccg accgtaatca
    13501 caaccgatgc gacatcgata taaccaccct tgttgttgat caggtagaaa aactggccgt
    13561 agtacctttg gttgggcgca cgaaagcgcc ctttgacagc gatgacaggt tcattcttga
    13621 gctccgacat tgttcttagg tacagctgcc cgcgcttacg tcaagccttt accctgaaaa
    13681 aaaccaggag aacctataca acggagccag ggaaaaaaac ggccacgggc gtcgtaatca
    13741 agtctggaag gtcgtcacat gtgccgccta aacctcaccg ggcgccagaa ggtggtcagt
    13801 acattgggca ataggcattt acagcatgtc agcagtccac ttttgcgctc atgaaccaag
    13861 aacgggattt cgtttttacc tctgaatcgg tttcagaagg ccatccggac aaagtctgtg
    13921 accagatctc tgatgcagtg ctcgacgcct acctcgagct tgacaatact actcgtgttg
    13981 cctgcgaatc gctcactact accgaccatg tgacagtagc cggcgaaatc tcgacttcat
    14041 ctaaaggcca cattaccaag gaaaaaattg agcaaatcgc gcgcgacgtg atgaaagaca
    14101 tcggttacgt gcatgacgat atcggttttg attaccgcac ggcaaatgtg cacatccttc
    14161 tgcacgagca atcgcaggat atcgcgcagg gtgttatcgg cgaaggcctt cataaagaag
    14221 aaggtgccgg cgaccagggc atgatgttcg gcttcgcgat tgatgaaacc gacgccctga
    14281 tgccaatgcc tatctactac tcgcacaaga ttcttgagca cctgacgggc ctgcgcaaga
    14341 gcggcaagct gcctttcctg cgtcccgatg ccaagtcaca ggtgactgtc cgctacgaaa
    14401 agggtaaacc cgccggcata cagactgtgg tcgtttcaac gcagcacacc ccggatgtcg
    14461 aacacagcaa actttccgag caggtgattg aagaatgcgt caaaaaggtg ctgcccgccg
    14521 agctgctcaa aggcacgaac tactacatca atcccaccgg tcgttttgtg gtcggcggtc
    14581 ctcacggtga ctgcggtctg accggccgca agattatcgt cgatacctac ggcggttatg
    14641 gtcgtcacgg tggtggcgct ttcagcggta aagatccctc gaaagtcgac cgcagcgccg
    14701 cctacatgat gcgttacgtg gcaaaaaacg ttgttgcttc tggtattgcc agccagtgcg
    14761 aaattcaggt cgcctacgcc atcggtgttg cgaagccggt ttcgcttgtc gtgaatactt
    14821 tcggtacggg caaggtgggc gatgacaaga tcgaaaaaat gatcatggaa aatttcgacc
    14881 tgactccgcg tggcatccgt gaaactctga acctgcgcaa tatcaagtac cgcccgacgg
    14941 cagcttacgg tcatttcggg cgtgaaggcc agaactcgtt tacctgggaa aaaaccgaca
    15001 aggcaggggt attcaaatag tcattggccg gcaaccccgg tctttgaact atttgacgcc
    15061 ctgagtcaga gtcgcaaatt gacggaaaga acaggaaagg ttagataaat gcccaatatt
    15121 gcttcagcta agaaacgcac ccgccagaat gaaaagcgcc gcctcaagaa ccaagcgcaa
    15181 aaatcggcaa tccgcacgca ggaaaaaaag gtgcgcgcac ttgtggctga caagaaagta
    15241 gacgaagcgg tcaaagcttc acagaagctg gcgtcgctgg tcgataaggc ttccaagact
    15301 aacctgatgc atgtgaatgc tgcttcgcgc aagaagtcac gcattgcaca actggtcaaa
    15361 aaggctgcag cggctcctgc cggctgaggc aaaaagtttc cttaaaggcg attagcacct
    15421 ccgcgatgcg cagcatcgct ccggagaggc ttcgcctcac agctggttag agcgcaggct
    15481 caggcagcaa agaacataaa gggcgattag ctcagctggt tagagcacct gccttacaag
    15541 cagggggtcg caagttcgaa tcttgcatcg cccacacttc cgcactgcag tcattccagg
    15601 gcgattagca cctccgcgat gcgtagcatc gcttcggaga gccttcggct cacatctggt
    15661 tagagcacct gccttacggt tcgacaagct caccaccagc aagc
//
LOCUS       DAOQJF010000025        15519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1803_length_15519_cov_5.166968, whole genome shotgun sequence.
ACCESSION   DAOQJF010000025 DAOQJF010000000
VERSION     DAOQJF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 15519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15519
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1803_length_15519_cov_5.166968"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLF85_02495"
     CDS             complement(<1..258)
                     /locus_tag="PLF85_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18554.1"
                     /translation="MLGKIFNFRFRLTAAIFLVAVGATVAGLIYYYQMASSQIWSQMS
                     SRVKDFGKLGVTLLTPADIKYLQTLDAKLNSAQHAPVLPPGV"
     gene            complement(248..1030)
                     /locus_tag="PLF85_02500"
     CDS             complement(248..1030)
                     /locus_tag="PLF85_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE18555.1"
                     /translation="MKTAQDFFLSLLPNATAISREVSAKFFKKPLEEIGMVSKADASP
                     VTAADIEIESRLRDLIMKQFPDHGIIGEEHGNHHTDAEYVWVLDPIDGTKSFITGVPL
                     FTTLIALMHKGEPVCGAIYQPVLDELVWGNNKECFFNDRPVRMRTAQNLADCTLLVTD
                     ARHVAEHHPKANFTGLSADVKFWRTWADGYGYALLAAGYADIMIDPVMNPWDIMAVVP
                     VVRGAGGTITDFAGADAVKGKSIVAASPGLHEQVLRRLGNAG"
     gene            complement(1040..1240)
                     /locus_tag="PLF85_02505"
     CDS             complement(1040..1240)
                     /locus_tag="PLF85_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18556.1"
                     /translation="MAQYSPGDKVKIRKPPHIFEGVIIMPGSPAEIAEVHEDGTLSLL
                     YHDREMMPHTMTGIKTDEIEKA"
     gene            complement(1272..2114)
                     /locus_tag="PLF85_02510"
     CDS             complement(1272..2114)
                     /locus_tag="PLF85_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNE18557.1"
                     /translation="MRSKTRTHYIIIAIALSASLQCASLAKKIFDPPQIGIDRVEIRN
                     VALTGADLVVHVKINNPNSVGATLNRLEYALEVDGERLVRGQKEDKTTIAANELSVIA
                     LPLTVNYAGMRAGLSGVLTKKMLPFNFSGKVVLDSPVGDLSFDIAEKGEIPIPDRPRF
                     DLTKIALAELGVTSATLMLHIKVTNNHDFELDIRRFRYEFSLQDNLVSASDITVERLV
                     GQDKSMSIVLPVTLKLLGLKKGVVDMLRSGQLKYAMKFHLDLNTRFGPVTVPYERDGL
                     TSLY"
     gene            complement(2114..2437)
                     /locus_tag="PLF85_02515"
     CDS             complement(2114..2437)
                     /locus_tag="PLF85_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HNE18558.1"
                     /translation="MSEALSQDDLKENIIAVIRTCYDPEIPVNVYDLGLIYGIDIDNE
                     KNVRIDMTLTSPNCPSIESLPSEIQQKAESVEGVKSVKIELVWEPPFHTGMMSDEAKL
                     QLGLM"
     gene            complement(2434..3297)
                     /locus_tag="PLF85_02520"
     CDS             complement(2434..3297)
                     /locus_tag="PLF85_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNE18559.1"
                     /translation="MALTQATGALALVSGHGKVSAHTCSISGTSAEITATGAGDLPID
                     AAALGQLDELQLNLTADSVQVFVPLTATERKLTISVKLAANTSTRLYLLARGPARNLQ
                     LVINAVDHARAGVYSLLDDAAAHFIYEANVQSGAEVTFTGLTRAQGKTVTGIEVNVRH
                     VAANGRTEQKFYSYARDTAQISFTGRIAVDPGASGTEAHQLHRGIALSPGARIDAQPF
                     LNIMHDDVRCTHGSTVGFIDEEALAYLMARGLGRNVAEQILIHSSERQFFDLVPTGEA
                     REFYGVKEEQL"
     gene            complement(3299..4048)
                     /gene="sufC"
                     /locus_tag="PLF85_02525"
     CDS             complement(3299..4048)
                     /gene="sufC"
                     /locus_tag="PLF85_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNE18560.1"
                     /translation="MSEPLLEISDLKVKTGDKEILKGINLKIQPGEIHAIMGPNGSGK
                     STLSHVLAGNPEFTVTGGSVKLSGKDLLEQPIEERARAGVFIAFQYPPEVPGVNNMQF
                     MRTAVNTVRKARGESELSAGDFLNRAKAVMARLNMQSEMIKRSLNDGFSGGEKKKNEI
                     LQMALLEPKIMVLDEIDSGLDVDALELVAKEAVNVMREGNGARSMVVITHYSRILKYI
                     EPQHVHILSGGKIIESGGMEIVHRIESNGYD"
     gene            complement(4096..5565)
                     /gene="sufB"
                     /locus_tag="PLF85_02530"
     CDS             complement(4096..5565)
                     /gene="sufB"
                     /locus_tag="PLF85_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNE18561.1"
                     /translation="MSTAAVEELKGEYKYGFVTDIEAETLPAGLSEETVRKISALKEE
                     PEFMLEWRLKAFRAWQTMQEPRWAEVNYPAIDYQKISYFSRPKKKLESIDQADPKLLE
                     TFNRLGIPLSEQKFLANVEGAEVNSRPATPVAVDAVFDSVSVVTTYRETLAKLGIIFC
                     SFSEAVREHPELVKEYLGSVVPVKDNYFSALNSAVFSDGSFVYIPPGVRCPMELSTYF
                     RINSANTGQFERTLIIADKDSYVSYLEGCTAPQRDENQLHAAVVELVALDRAYIKYST
                     VQNWYPGDENGKGGIYNFVTKRGLCKGTDSRISWTQVETGSAITWKYPSCILKGDNSK
                     GEFYSVAFTGNYQQADTGTKMIHLGRNTSSTIISKGISAGHGKNTYRGLVRIGGAAEK
                     ARNYTRCDSLILGGTAAAHTYPYIEVKNEKSQVEHEATTSKVSDAQLYFLRQRGLTEE
                     DAVHMVVNGFCKEVFRELPMEFAVEAQKLLEISLEDANG"
     gene            complement(5639..6673)
                     /locus_tag="PLF85_02535"
     CDS             complement(5639..6673)
                     /locus_tag="PLF85_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18562.1"
                     /translation="MRRFLLLTIISALPECGSVQARTPVLPAETKPSAVPTERPAEKP
                     APQKSNLVPGAPNTQATAETPKKKTLPKVQNPLLNPYEASAFFDHQAEEHQRFGIPRY
                     AKSQKKKKYGNSVVMQRSGRRRLYDHNILLRALFAGRLHSEVYGDLAEQFTGGIFFDL
                     GSAVLFGEGAETVRDLHEDEKIRDKLTIIASDINDPGGKKSMFVDQYRESDEQLPFPV
                     VEIPRLMDKPEHFSRPLRLFLNNKSGIILRSANSGPDLYYDTNQVKAHLRAAIQAFPE
                     RNILYLFNKYILYKPAARFDFLLIGEIDETVGINHKQPVWEEIDWSKRKFAEAVRLNT
                     QHVFFRPQGQ"
     gene            6765..7118
                     /locus_tag="PLF85_02540"
     CDS             6765..7118
                     /locus_tag="PLF85_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNE18563.1"
                     /translation="MPARGYVNEAEDIAVRFLRASEITVLSRNDRRFGAEMDIVGRPK
                     GEGELCIFEVKQWQKNAAFPAVSAAQRKRLAAAIGSLEGEAGSRLPVVLYALLVNTTR
                     QSICLVPLADYSGTC"
     gene            complement(7123..8241)
                     /locus_tag="PLF85_02545"
     CDS             complement(7123..8241)
                     /locus_tag="PLF85_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNE18564.1"
                     /translation="MSTDHHAGLEDIEVLESGICEIRQDKLSFRGYNITDLAENAGFE
                     ETAFLLWNDALPSAQELSDFKTSLRAEYNLPREITEHINHVPREAHPMAVLRTLISSL
                     GLYDPEASDNSPPALRRRSTRLLAKIPLIVAAIARHRNARSHVDANPDLDIAANFLHC
                     LYGEAPDTQTARLLETALVLYAEHELNASTFAARVTAATQADTYSCIVAAVSALQGNL
                     HGGATQRSVEMLLKIGHSEDAESYILPRLKKKEIIMGFGHRVYKQGDPRVPVLKRMCT
                     QLAQKTGQQQIVETAAKAEEIMLREKKLHANVDYYAGLAFYLLGLGADLFTCVFALSR
                     TAGYLAHIAEQYRENKLIRPRGVYTGKKDLSFMPLAAR"
     gene            8323..8826
                     /locus_tag="PLF85_02550"
     CDS             8323..8826
                     /locus_tag="PLF85_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18565.1"
                     /translation="MMRITVILLLLAGSLVSAVGAGEKIFKNRKLRITFEYPAEWSVN
                     EDADGVKVSSPDGAASVSIRALATKEKVSACEILKTRSEELKLTNLLPDDKRVVTEDQ
                     LRFLGVKDGCLGAFQIVEGDTEVLSGVGLYTSGKRLWLLEQRLRIGQHEKHSAAISAV
                     AASFTAN"
     gene            8945..11491
                     /gene="sppA"
                     /locus_tag="PLF85_02555"
     CDS             8945..11491
                     /gene="sppA"
                     /locus_tag="PLF85_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNE18566.1"
                     /translation="MIEFQLRCFSAAMPGQSPLGRPVHGYSWLKLVLVGGLLFSGFFP
                     AAAQIAPGQSDALQTNRMIDTPNLPQKSVATSDDTTSLYFNPAGLGYHALQLGYFYGR
                     NPQNRLDDHMVFLNLLGIAFSTQWRLGNSNDYARRYTVGMGIINTGSFSVGTSYSWYD
                     SSFAYLDRYNQWDLGFIFRPWRRLSFGAVGRALNKPLFRGEIFETNWDLGVGIRPLPG
                     KYVENLTLSLDANLNSTTSWGTVTPKFIAEASLWPGNTIYGGYNQNTGAFFGLRFAQY
                     ISQLSLQANTPTDNGAFLGGGLLIGRERYATGISALGRFLEINLEDPVPERKQEGFLF
                     LSENFTFLELVRTIEMAKKDAQIRGILLTGREFKGGWGQAEELRAALLSFQQKKPVYA
                     YLENAGNKEYYIASMSDRIFMPQSAMLDISGLKAEAYFVKDLLAKVGVKADFVALGDY
                     KSAPDRLTRSEPTKFDREQLEGLLKSGVAEMKAAISAGRLRVKEEKVDEIMDKGLFSA
                     TRAQEAGLIDEVAYMSDVENQLTAQGFSAPGWRVSAPDYAQTRFYDDTWGTKPTIAVL
                     VLAGEIMSGPSRSEGLFNNGTIGSDTIEKVLEQIKDDGNIKGLVLRIDSPGGSSLASD
                     IMWNKIREVKKARGADFPIVVSIGNMAASGGYYLAVGGDEILASHNSITGSIGIFTGK
                     FNVKGLYDWLGVRKHTFKTHKNGAIFSESEGFTDEEKLLIKEHLTEFYDLFLRRVADN
                     RGTSPEQVKPVAGGRVWSGKDALAKKLVDKHGGLLLALQLVREKAEIDENYYQLQVFP
                     SDAQNLLSLGDPRKLMLPGILREAARLVGKTESIKDEKVLFLMPYDIEVR"
     gene            11493..12350
                     /locus_tag="PLF85_02560"
     CDS             11493..12350
                     /locus_tag="PLF85_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNE18567.1"
                     /translation="MPGQTRILCIPFVPVLGAKSRNLTLLSELAERIAASAEKPDVVV
                     LPELALSGYLLESLVSEVALVESELSEIANILAGSGVPAHTEWVIGLPLREAGEIYNC
                     AVVFRGGEVRHIHRKLFLPTYGMFDESRYLARGTELTTFAGALGRTALLICEDAWHME
                     LAFAVSAKKADAVIVISASPARGLTQAAEFTSSARWRHRLQTFAESYGQKYVYCNRGG
                     VEDGVLFDGTSFVFDPLADYIAAEQSEIYEAARLYRVNTSTGMRQGFLGNSARQNDVL
                     MLQRLLSGS"
     gene            complement(12373..13044)
                     /locus_tag="PLF85_02565"
     CDS             complement(12373..13044)
                     /locus_tag="PLF85_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18568.1"
                     /translation="MAFGRKKKEKPAEEQSVLDSATSDELFIVDQVRKVQVQRLHKTY
                     ADFFENPEYTKISRYFFDQIYGAGDKASRDAAFNKIYEKLSAAVNPKRITRVTQLKQL
                     NELTDELDLRVAREFIRAHKKHAMTLEDFEACYRALNNKDEREVQLQLLIDTTRFFHK
                     LAHVPLLGFVIKPTQLAARALGVEYIMEFFMEGYRSFKSVKNAEPFMQAIEDRERAYL
                     NSLLP"
     gene            complement(13053..13844)
                     /locus_tag="PLF85_02570"
     CDS             complement(13053..13844)
                     /locus_tag="PLF85_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE18569.1"
                     /translation="MSYKFLSIENKTTTLKSGANAGYAIVTLNIPPMNALASGVFHDL
                     DAAITSLESSQTRVAIVTGAGKAFVAGADISEMNGMDVAKAKEYSLLGHRIFSRMETS
                     PVIFIAAVNGFALGGGLELAMAADIRIFAEAAQVGLPEPTLGLIPGFGGTQRLQRLVG
                     QSNAAYLSLTSERINAQEALRIGLAAKLTSAENLMPECEAVAQKILTSGPQAIRVLKH
                     TILNGADKPMPQALEFEAQEFSQLFAGGEAHEGLKAFLEKRPAKF"
     gene            complement(13905..14993)
                     /locus_tag="PLF85_02575"
     CDS             complement(13905..14993)
                     /locus_tag="PLF85_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE18570.1"
                     /translation="MDKKISAVLDSVLAGNRLSDADALLLWREANFRDLGNVAVEIRN
                     RLNPANEVTYTAFRVVNYTNYCNIECSFCSFMDEVGSGKGYNLSKDEILKKVEESIKL
                     DAPQLFLQGGVNPLLPMDYYFDILSAVRSQYPEVHIRAFSPVEVLFMEKLTQKPLSEV
                     FRELADAGMNSFPGAGAEILTDRMRDILSPKKASTAEWLRAMRTAQQQGMKSSTNIVW
                     GSEEEDTEIIAHLSALRSLQDETGGILSFVPWTFQAQTKKFRIRRVPPHEYLKMVALS
                     RIYFDNIPHIEVSVMVAGKQSGEIALHFGGDDINSPVIEENVLRSFGLKSVEESERFI
                     ADAGFTPVRRSFNYIRGQKSDLGSALPA"
     gene            complement(15040..>15519)
                     /locus_tag="PLF85_02580"
     CDS             complement(15040..>15519)
                     /locus_tag="PLF85_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18571.1"
                     /translation="ARKEVIIHELHVRAGDEFWGGALRLMIAGRAGATNFSLPGESQD
                     VIQSFSVGGPEARYRRVAGYAFSEFRAPAFGLVNLDAIVHAGGPVNLWFVADAVTFDR
                     EFNGQRFHAGAGAGLIFDLPQGMLGSRSALFARAEVPFFAAGGNRFQVFMGMNGQVF"
BASE COUNT         3277 a         4087 c         4400 g         3755 t
ORIGIN      
        1 cacaccgggc ggcagcacag gcgcgtgttg tgcggagttc agcttggcat cgagggtttg
       61 caggtacttg atatctgccg gcgtcagaag ggttactccc agcttgccga aatctttcac
      121 gcggctcgac atctgtgacc agatttgcga gctcgccatc tggtaatagt aaatcaggcc
      181 tgcaacggtg gcgccgaccg cgaccaggaa gatcgcggca gtcaggcgaa agcggaaatt
      241 aaaaatctta cccagcatta cccaggcgcc gcagaacttg ctcatgcagg ccggggctcg
      301 cggcaacaat agatttgcct ttcacggcat cagctccggc aaagtctgtg atcgtcccgc
      361 cggcgccgcg tacgacgggc acaacggcca tgatatccca ggggttcatg acggggtcaa
      421 tcatgatatc tgcataaccc gctgcgagta gcgcgtagcc gtaaccgtcg gcccaggtac
      481 gccagaattt cacgtcggca ctcagacctg taaaatttgc tttggggtgg tgctcggcca
      541 cgtggcgtgc gtcggttacc agcagcgtgc agtcagcgag attttgggcg gtgcgcatcc
      601 gaaccgggcg atcgttgaag aaacattctt tgttattgcc ccagacaagc tcgtcgagta
      661 ccggttggta gatggcaccg cagaccggct cacccttgtg catgagggcg atcagtgtgg
      721 taaagagtgg cacgccggtg atgaaactct tggtgccatc gatagggtcg aggacccaga
      781 catactccgc gtctgtgtgg tggttgccgt gttcttcacc gataatgcca tgatcgggaa
      841 actgcttcat gatcagatcg cgcaggcgcg attcaatttc aatatccgcc gcagtcacgg
      901 gcgaggcgtc ggctttgctg accatgccta tctcttcgag tggcttcttg aagaattttg
      961 ccgaaacctc gcggctgatc gctgtggcat tcgggagcag ggagagaaaa aaatcctggg
     1021 cggttttcat gggtaatcct tacgcttttt ctatttcgtc ggttttgatg cctgtcatcg
     1081 tgtgcggcat catttcgcgg tcatggtaca agaggctcaa ggttccatct tcatgcactt
     1141 cggcaatttc cgcaggtgaa cctggcatga tgatcacccc ctcaaagatg tggggtggtt
     1201 tccgaatctt taccttgtct ccgggactgt actgcgccat aacgcagaac gcaattccat
     1261 aagtgcaata gtcagtaaag ggaagttagg ccatcccttt catacggaac agtcaccggc
     1321 ccaaacctgg tgttgagatc caggtggaat ttcatcgcgt atttgagctg tcctgaacgc
     1381 agcatatcga ccacaccctt cttgagccca agaagcttca gcgtgacagg cagcacaatg
     1441 gacatcgatt tatcctgtcc caccaggcgc tcgacggtga tatctgacgc agaaaccagg
     1501 ttatcctgca gcgaaaactc gtaccggaaa cggcggatgt caagttcaaa atcgtggtta
     1561 ttggtgacct tgatgtgcag catcagggtg gcagaggtta cgcccagttc agcgagcgcg
     1621 atcttggtca ggtcaaaacg tggcctgtcc ggtattggga tctccccctt ttctgcaatg
     1681 tcaaaactca ggtcgccaac gggtgaatcg agcaccacct tgcccgagaa attaaagggc
     1741 agcatcttct tcgtgagcac cccggaaagc cctgcgcgca tgccggcgta gttcaccgtc
     1801 aggggcagtg caatgacact gagctcattt gccgcgatgg ttgttttgtc ctctttctgg
     1861 ccccgcacga gtcgttcgcc gtcgacttcg agtgcatatt cgagtcggtt aagggtcgcg
     1921 ccaacagaat tgggattatt aatttttacg tgcacgacga ggtctgcgcc tgtcagcgca
     1981 acgttcctga tctcaacccg gtcaatgccg atttgcggcg gatcaaagat ttttttcgcc
     2041 agcgaagcgc actgcaggct tgcagacagc gctatcgcaa tgattatgta gtgggtcctg
     2101 gttttgcttc gcatcacatg agtccgagtt gcagttttgc ttcatcggac atcatgccag
     2161 tatggaatgg tggctcccat accagttcta tttttacaga ttttacgccc tcaacgcttt
     2221 ctgccttctg ttgtatttca gagggcaatg attcaatcga tgggcaattg ggagaagtga
     2281 gtgtcatgtc gatgcgcaca tttttttcat tgtcgatgtc gataccgtag attagcccga
     2341 ggtcatagac attgacggga atttccggat cataacacgt gcggatgacg gcaataatat
     2401 tttctttcag gtcatcttgt gacagcgcct cgctcacagc tgctcctctt tgactccata
     2461 gaattcacgc gcttcgccag tcggaaccag gtcaaaaaac tgccgctccg aagaatgtat
     2521 caggatctgt tctgcaacat tgcgcccgag cccgcgggcc atcaggtagg caagtgcctc
     2581 ttcgtcgatg aaacctactg tcgaaccatg cgtgcatcgc acgtcgtcat gcatgatatt
     2641 caggaaaggc tgtgcatcga tgcgcgcgcc tggtgaaagg gcaatgccgc ggtgcagctg
     2701 gtgtgcttct gttccacttg ccccgggatc aacggcaata cgcccggtaa agctgatctg
     2761 cgctgtgtcg cgggcatagg agtagaattt ctgttccgtc cggccgttgg ccgctacgtg
     2821 ccttacattg acttctatgc ccgtgacagt cttgccttgt gcgcgggtga gtcccgtaaa
     2881 tgtaacttct gcaccggact gcacattggc ttcgtagatg aagtgcgccg cggcatcgtc
     2941 gagcagcgag tatacgccgg ctcgtgcgtg gtcaaccgcg ttgattacca gttgcaggtt
     3001 gcgtgcaggg ccccgggcaa gcaggtatag cctggtgctg gtattggcag caagtttaac
     3061 agaaattgtc agcttccttt cggttgccgt taacggaaca aaaacctgca cagaatctgc
     3121 agtaaggttc agttgcagtt cgtccagctg gccaagggca gcagcgtcga tcggcaaatc
     3181 accggcaccg gttgcagtta tttctgcgct cgttcctgaa atactgcatg tatgggcaga
     3241 gactttgcca tgcccggaaa ccaatgcaag ggcgcctgta gcctgcgtca gcgccatgtc
     3301 agtcgtaccc gttgctctcg atccggtgga cgatctccat gccgcctgat tcgataattt
     3361 ttccgcctga gagtatatgc acatgctgcg gctcgatata tttcaatatg cgcgagtagt
     3421 gcgtgatcac taccatgctc cgtgcaccat tgccctcacg catgacattg actgcttctt
     3481 tggccacgag ctcaagtgca tcgacatcta ggcctgaatc gatttcgtcg agtaccataa
     3541 ttttcggctc gagtagtgcc atctggagaa tctcgttctt ttttttctcg ccgcccgaga
     3601 aaccgtcgtt gagcgaccgc ttgatcattt cgctctgcat attcaggcgg gccataactg
     3661 ccttcgcacg gttcagaaag tcgcctgctg aaagttcact ttcgccgcgc gccttgcgca
     3721 ccgtgttcac tgcggtccgc atgaactgca tgttattgac ccccgggact tcgggcgggt
     3781 attggaaggc aataaatacg cccgcgcgtg cgcgttcttc aatcggctgt tcaagcaggt
     3841 ctttgcctga aagtttaacc gaaccaccgg tgacggtaaa ctccgggttg ccagcaagta
     3901 cgtgcgacag tgtgctttta cctgagccgt tagggcccat gattgcatgt atttcgccgg
     3961 gttgaatttt caggttgatt cctttcagaa tctccttgtc cccggttttt accttgaggt
     4021 ctgatatttc cagcagtggt tcgctcattt cttctctttg ctcaaaaaaa tagtacggtg
     4081 gattttcaaa aggtttcagc cattggcgtc ctcgaggctg atctcgagca gtttctgcgc
     4141 ttcaaccgca aattccatgg gcaattcacg aaagacttct ttgcagaagc cgttcacgac
     4201 catgtgcact gcgtcttctt cggtaaggcc acgctggcgc agaaagtaga gctgcgcatc
     4261 gctgaccttc gaagttgtgg cttcgtgttc aacctgggat ttttcgttct ttacttcgat
     4321 gtaagggtag gtgtgcgcag ccgcggtacc gccgagaatc agcgaatcgc agcgcgtgta
     4381 attacgcgcc ttttctgcag caccaccgat acgcaccagg ccgcggtacg tgttcttgcc
     4441 gtgcccggct gaaatccctt tggagatgat cgtgcttgag gtgttgcgac cgagatgaat
     4501 catcttggtg ccagtgtctg cctgctggta attgccggtg aaggcaacag aatagaattc
     4561 gcctttcgag ttatcgccct tgagtataca gctcgggtat ttccacgtga tcgctgaacc
     4621 cgtttcaacc tgtgtccagg aaatgcgcga atcagtaccc ttgcagaggc cgcgcttggt
     4681 gacaaagttg tatataccac ccttgccatt ctcgtcgccg ggataccagt tctgtaccgt
     4741 tgagtatttg atgtaggcgc gatcgagcgc cacgagttca acaacagcgg cgtgcagctg
     4801 gttttcatcg cgttgcggcg ccgtgcagcc ctcgaggtag ctgacatagc tgtccttgtc
     4861 ggcaatgatc agcgtacgct cgaactgccc tgtatttgcg gagttaatgc ggaaataggt
     4921 ggagagctcc atcgggcagc gaacgcccgg cggaatgtag acaaacgagc cgtcgctgaa
     4981 taccgcgctg ttcagggcgc tgaagtagtt atctttaacg ggtacgactg agccgaggta
     5041 ttctttcacg agttcgggat gctcacgcac tgcttcagag aacgagcaaa aaataatgcc
     5101 cagttttgcc agggtttcac ggtatgtcgt aacaacggag acactgtcga atacggcatc
     5161 tacagccaca ggggtggctg gccggctgtt cacctctgct ccttcgacgt tggccagaaa
     5221 tttctgctcg ctgagaggaa tgcccagccg gttaaaggtc tcaagcagct tggggtctgc
     5281 ctgatcgatg gattccagtt tcttcttggg gcgcgaaaaa tagctgattt tttggtaatc
     5341 gatagcaggg taattgacct ctgcccaccg tggctcctgc atggtctgcc aggcgcgaaa
     5401 ggccttcagg cgccattcga gcatgaattc aggctcttct ttcagtgcgg agattttacg
     5461 tactgtttct tcggagagac cagcaggcag ggtctctgct tcgatatcgg taacgaagcc
     5521 gtatttatat tcacctttga gttcttccac agcggcggtc gacatgcacg ctaaatatac
     5581 taaaccgctt tgtaaaagga tagttatttt ttagacgaac tatagacaag aaaatccgtc
     5641 attggccctg cgggcgaaaa aaaacatgct gcgtgttcag ccgcactgct tcggcaaact
     5701 ttcgcttcga ccagtctatt tcttcccata ccggttgttt atggtttatg cctaccgttt
     5761 cgtcgatttc gcctatcagc agaaagtcaa agcgcgcagc aggtttatag agaatgtact
     5821 tattgaagag gtacagtata ttccgctcgg gaaaagcctg gatcgcggca cgcaggtgag
     5881 cttttacctg gttggtgtcg tagtacaggt cgggaccgga gttcgcgctg cggagtataa
     5941 tgccgctctt attgttgagg aagagcctga gcgggcggga aaagtgctcc ggcttatcca
     6001 tgaggcgcgg tatttcgacg acaggaaagg gcagctgttc gtctgattcg cggtactggt
     6061 cgacgaacat actcttcttg ccacccgggt cattgatatc gctggcgata atcgtcaact
     6121 tgtcgcggat tttttcatcc tcgtgcaggt cgcgcaccgt ttcagctcct tcgccgaaca
     6181 gcaccgcgct acccaggtcg aaaaaaatgc cgcccgtgaa ctgctctgcg agatcgccgt
     6241 aaacttctga atgcaggcgg ccggcaaaaa gcgcccggag caggatattg tggtcgtaca
     6301 gccgcctgcg ccccgagcgc tgcatgacaa cactgttgcc atatttcttt ttcttctggc
     6361 tttttgcata acgcggaatt ccgaagcgct ggtgctcctc tgcctggtgg tcaaaaaagg
     6421 ccgaagcctc gtacgggttg agcagggggt tttgcacttt tggcaatgtc ttcttcttcg
     6481 gcgtttcagc ggttgcctgc gtgttcgggg cgccgggtac caggttggat ttctgtggtg
     6541 ccggtttttc tgccgggcgt tcagtcggta cagcactggg ttttgtctct gccggcaata
     6601 cgggagtccg cgcctgcacc gaaccgcatt caggcagtgc tgagattatt gttagtaaaa
     6661 gaaaccggcg catgctccta aatcggtgcc atcgatagaa tttcaagtgt attgattgcc
     6721 atacaatgta gtttacgtcg gtagcccggc gctaccctga ggttatgcct gcgcgcggct
     6781 atgttaatga ggcggaggat atcgcggtac gatttctgcg ggcctctgaa ataaccgtgc
     6841 tttcccggaa tgatcgcaga tttggtgcag aaatggatat tgttggtcgt ccaaaaggtg
     6901 agggggagct ctgcattttt gaggtcaaac aatggcagaa gaatgctgct tttcccgctg
     6961 tgtccgcagc gcagcgtaaa aggctggctg ctgccatagg ttcgctcgag ggtgaagctg
     7021 gcagtcgcct gccggtagtc ctgtatgcgc tgctggtcaa tactacgcgg cagtcgatat
     7081 gcctggtgcc gcttgccgat tattccggaa cctgctagtt tgctaacgtg cggcaagagg
     7141 catgaatgac aggtctttct tgcctgtata gacaccgcgc gggcgaatga gtttgttctc
     7201 gcggtactgc tcggcaatat gtgcgaggta gcctgcagtg cgcgaaaggg cgaagacgca
     7261 ggtaaaaaga tcggccccca ggcccagtaa ataaaatgcg aggcctgcgt aataatcgac
     7321 attcgcgtgt agtttttttt cacgcagcat aatctcttca gccttcgcgg cagtctcgac
     7381 gatctgctgc tggccggtct tctgtgcaag ctgcgtgcac atacgcttca gcaccggaac
     7441 ccgcgggtca ccctgcttgt acacgcggtg accaaatccc atgatgattt ctttcttctt
     7501 taatctcggg agaatatagc tttctgcgtc ctcgctgtgt ccgatcttca gcagcatttc
     7561 tacggagcgc tgtgttgctc ccccgtgcag attgccctgg agtgcgctca ctgcagcgac
     7621 tatgcacgaa taggtgtctg cctgtgtggc ggccgtcacg cgtgccgcga atgtcgatgc
     7681 attgagttca tgttccgcgt agagcacgag ggcggtctcg agcagccgcg ctgtttgagt
     7741 atccggggct tctccgtaga ggcaatgcag aaagttcgca gcaatatcca ggtcagggtt
     7801 tgcatcgacg tgagaacgcg cattgcggtg ccgggcaatc gcagccacaa tcaggggaat
     7861 ttttgccaga agcctggtcg agcggcggcg cagcgccggc ggcgagttat cggaggcttc
     7921 aggatcgtag agtcccagcg aagaaatcag cgtgcggagc actgccatcg ggtgggcttc
     7981 gcgcggtacg tggttgatgt gttcggtaat ttcgcgcggc aggttatatt cagcgcgcaa
     8041 ggatgtctta aagtcgctga gctcctgcgc ggatggcaga gcgtcgttcc agagcaggaa
     8101 tgccgtttct tcgaaaccgg cattttcggc caggtcggta atgttgtagc cgcgaaagga
     8161 aagcttatcc tgcctgatct cgcagatgcc cgattcgagt acttcgatat cttcaagccc
     8221 ggcgtggtgg tcagtcgaca taaatctttt tcggtgccat gcgtagcacg gcaagtggaa
     8281 aaaatcttgc cgctgccgtt ctgtttatcg catcttgcac ctgtgatgcg tattactgtg
     8341 attctattgc tgctagccgg ctctttggtg tcggcagtcg gtgccgggga gaaaatattc
     8401 aagaaccgta agctccgcat tacctttgag taccctgccg agtggtcggt gaacgaagat
     8461 gccgatggcg tgaaggtgag cagccctgac ggcgcggcca gcgtgagtat tcgtgccctc
     8521 gcaacaaaag aaaaagtttc agcctgtgag attctgaaga cacgcagcga agaattgaag
     8581 ctcacgaatc tgttgcccga tgataagcgc gtcgtaacag aagaccagct gcgctttctt
     8641 ggcgtgaaag acggctgtct cggtgctttc cagattgtcg aaggagacac agaggtactg
     8701 agtggtgttg gtctttatac gagtggcaag cgtctctggc tgctcgagca gcgcctgaga
     8761 attggccagc acgaaaagca cagtgccgct atcagcgccg ttgcagcctc atttactgca
     8821 aattagcagt tgcctgaaca acgaacctgc ggctttctta ccgcgcgaga tgcgtcatta
     8881 acgcgctctc gtgcggctga agttgcgctc gacacactgt ctcgcaggcc attattttcc
     8941 gaaaatgatc gaattccaac tgcgctgttt ttctgcagcg atgcctggcc agagtccgtt
     9001 gggccggccg gtgcatggtt atagttggtt aaaactcgta ctggtaggcg gcctgctttt
     9061 ctcaggcttt tttcccgctg cggcacagat tgctccgggg cagagcgatg cgctgcagac
     9121 gaaccgcatg atcgatacgc ctaatctgcc gcagaaatcc gtagcaactt ctgacgatac
     9181 cacgagcctc tatttcaacc ctgccgggtt gggctaccac gctctgcaac tgggttattt
     9241 ctatggccgc aatccgcaga accgcctcga cgaccacatg gtttttctga atcttctcgg
     9301 tattgctttt tccacgcagt ggcggttggg caacagcaac gactatgccc ggcgctacac
     9361 agtgggcatg ggtatcatca acacgggttc tttctctgtt ggtacgtcgt acagttggta
     9421 cgattcttcc tttgcatacc ttgaccgcta caaccagtgg gatctcggtt ttatcttcag
     9481 gccgtggcgc aggctgtcat tcggagccgt cggccgtgcg ctcaataagc cactgttccg
     9541 cggggaaatt ttcgaaacca actgggacct cggcgtcggc atccgtccgt tgccgggcaa
     9601 gtatgtcgag aacctgacgc tctcgcttga cgcaaacctc aattcgacga caagctgggg
     9661 taccgtcaca ccgaagttta tcgccgaggc atcgctctgg ccaggtaaca cgatctacgg
     9721 tggttacaac cagaacaccg gcgcattttt tggtctgcgc tttgcgcagt atatcagcca
     9781 gctctccctg caggcaaaca ctcccaccga taatggcgca tttttgggcg gcggtttgct
     9841 tatcggtcgc gaacgttatg ccaccggcat ttctgcgctc gggcgttttc tcgaaatcaa
     9901 tctcgaagat cccgtgccgg agcgcaaaca agagggattt ctcttcttgt cggagaattt
     9961 cacctttctt gaactggttc gcacgattga gatggccaaa aaagatgcgc agatcagggg
    10021 aattctgctc accggtcgcg agttcaaagg cggctggggc caggccgaag agctacgagc
    10081 agcgctgctt agcttccagc agaaaaagcc ggtttatgca tacctcgaaa atgccggcaa
    10141 caaggaatac tatatcgcga gtatgtcaga ccgcatcttt atgccgcagt cggcgatgct
    10201 cgacatatcg ggcctgaaag ccgaagccta cttcgtcaaa gacctgctgg caaaagtcgg
    10261 cgtgaaggct gatttcgttg ccctcggcga ttataagagc gctcccgacc ggctgacccg
    10321 ttcagagccg acgaagtttg accgtgaaca gctcgaaggc ctgctcaagt cgggagtcgc
    10381 cgagatgaaa gcggcgatca gcgccggccg cctgcgggtg aaagaagaaa aagtcgacga
    10441 aatcatggat aagggcctct tctcggcgac gcgcgcgcag gaagcggggc tcatcgacga
    10501 agttgcttat atgagcgatg tcgaaaacca gctgactgca caggggttct ctgcacccgg
    10561 ctggcgtgtt tccgctcctg attatgcgca gacgcggttc tacgacgata cctggggtac
    10621 caaaccgaca atcgcagtac tggtgctggc aggcgaaatt atgtcgggcc catcgcgctc
    10681 cgaaggcctt ttcaataacg gcacaattgg ttctgatacc atagaaaagg tgctcgagca
    10741 gatcaaagac gacggcaaca tcaagggtct tgtgctgcgc attgattctc ccgggggctc
    10801 gagcctggcc tcggacatca tgtggaacaa aattcgcgag gtcaaaaaag cgcgcggcgc
    10861 tgatttcccg atcgtcgtgt cgatcggtaa catggccgct tcgggcggtt attaccttgc
    10921 ggttggcggc gacgaaattc tcgccagcca caacagtatc accggttcga ttggtatctt
    10981 tacgggtaag tttaacgtca aggggctcta tgactggctg ggagttcgta agcacacgtt
    11041 caagacacat aagaacgggg caatcttcag cgaatcagaa ggttttaccg acgaagagaa
    11101 gttactcatt aaggaacacc tgaccgaatt ctacgacctg ttcctgcgcc gtgttgccga
    11161 caaccgtggt accagccccg agcaggtaaa gcctgttgcc ggtggtcgcg tctggagtgg
    11221 caaagatgcg ctagccaaaa aacttgtcga taagcacggt ggactgctgc ttgcactgca
    11281 gctggtgcgc gaaaaagccg aaatcgatga gaactactac cagctgcagg tttttccgag
    11341 tgacgcgcag aacctgctgt cgctgggcga tccgcgcaag ctgatgctgc cgggtatact
    11401 gcgcgaagcc gcacggctcg ttggcaagac cgagagcatc aaagacgaga aagtcctctt
    11461 cctgatgccg tacgatatcg aggtgcgctg agatgccagg ccaaacaagg atcttgtgta
    11521 ttccttttgt tcccgtgctg ggcgcaaaat cgcgcaacct gacgctgctg tctgagctcg
    11581 cggagcgtat agcggcgtca gcggaaaaac cggatgttgt tgtcctgccg gaacttgcgc
    11641 tcagcgggta cctgctcgaa agcctcgtga gcgaggtggc gctggtcgag agtgaactca
    11701 gtgaaatcgc caatattctt gcgggttcgg gcgtaccggc gcacaccgaa tgggtgatcg
    11761 gcctgccgct gcgcgaggcg ggtgaaatct acaactgtgc cgtggtcttt cgcggcggcg
    11821 aggtccgcca tatccaccgc aaactctttc tgccgaccta cggtatgttt gacgaatctc
    11881 gttaccttgc gcgcggtact gaactcacga ccttcgcagg tgcgctgggc aggacagcac
    11941 tcctgatctg tgaagatgcc tggcacatgg aactggcatt tgccgtttct gctaaaaaag
    12001 cagacgcggt catcgtcatt tctgcctcgc cggcgcgcgg cctgacgcag gccgcggagt
    12061 tcacctcttc ggcacgctgg cgccaccgtt tgcagacttt cgccgaatcg tacgggcaga
    12121 agtacgtcta ctgcaaccgc gggggtgttg aagatggtgt gctgttcgat ggaacaagtt
    12181 tcgtctttga cccgctcgcg gactatattg cggcagagca gagtgaaatt tatgaagctg
    12241 cgcggctgta ccgcgttaat acttctaccg gcatgcggca gggttttctc ggtaactcag
    12301 cgcgacagaa cgatgtgctg atgctgcagc gactgttgtc aggctcctga aaaagcagcc
    12361 aaaagcaggt gttcagggta gcagtgaatt gaggtaggcg cgttcgcggt cttcaatggc
    12421 ctgcatgaag ggttcggcat tcttcactga tttgaacgaa cggtagcctt ccatgaaaaa
    12481 ttccatgatg tattcaacgc cgagcgcccg cgcagcgagc tgcgtaggct tgataacgaa
    12541 accaagcagg ggcacgtgcg ccagcttatg gaagaaccgc gtcgtatcga tgagcagttg
    12601 taattgtact tcgcgctcgt ctttgttatt gagagccctg taacaggctt caaaatcttc
    12661 gagcgtcatt gcgtgtttct tgtgcgcgcg aatgaattcc cgcgccaccc gcaggtccaa
    12721 ttcgtcggtc agctcgttga gctgtttcaa ctgcgtcacc cgggtgatgc gcttggggtt
    12781 caccgcggcc gaaagtttct cgtagatctt gttgaatgcc gcatcgcggc ttgctttatc
    12841 cccggcgcca tagatctggt caaaaaaata gcgcgagatc ttggtgtact cggggttctc
    12901 aaaaaaatcg gcataggtct tgtgcaggcg ctgtacctgt actttgcgca cctggtcgac
    12961 gataaaaagc tcgtcagaag tcgccgagtc gagaaccgac tgctcttctg ccggcttttc
    13021 ttttttcttc ctgccgaaag ccatattgcc cgtcagaatt ttgccgggcg tttttcgaga
    13081 aatgccttga ggccttcgtg cgcctcgccg ccggcaaaca actgtgaaaa ctcctgcgct
    13141 tcaaactcga gcgcctgtgg catcggcttg tccgcaccgt tgaggattgt gtgcttcagt
    13201 acgcgaattg cctgcggacc cgaggtcaga attttctgcg ctaccgcttc gcattctggc
    13261 atcaggtttt cagcgctggt gagtttggct gcaaggccga tgcgcagcgc ctcttgtgcg
    13321 ttgatgcgct ccgaggtgag cgaaaggtat gctgcattgc tctgcccgac gaggcgctgc
    13381 agacgctgtg tgccgccaaa gccgggtatc aagcccagtg tcggctcagg caggccgacc
    13441 tgtgctgcct cggcgaagat gcgaatatca gccgccatgg cgagttcgag tccgccgccg
    13501 agggcaaagc cgtttactgc tgcaataaag atgacgggag aagtctccat gcgggaaaag
    13561 atgcggtggc cgagcagcga atattctttt gccttggcga catccatgcc gttcatttcg
    13621 ctgatgtcgg cgcctgcaac gaatgccttt cctgcgccag taacaatggc cactcgcgtc
    13681 tgtgaagatt cgagagaggt aattgccgcg tcgagatcat ggaatacacc agaggcgagc
    13741 gcattcatcg gcggaatgtt cagcgtaaca atggcgtagc cggcgttggc gcctgatttc
    13801 agcgtggtgg tcttattttc aatcgaaaga aacttatatg acatggcgtg tgaaacacgt
    13861 tttgagacgc agtgtcaagg cgttgtaagg cccggtcttg tccatcaggc gggtagagcg
    13921 ctgccgaggt ccgatttttg ccctcttata tagttaaagc tacgcctaac cggtgtgaag
    13981 cccgcgtcgg caataaatcg ttctgactct tcaaccgact taaggccaaa cgaacgcaac
    14041 acgttctctt cgatgaccgg tgaattgata tcgtcgccgc cgaagtggag cgcaatttca
    14101 cccgattgtt ttcccgcaac catgactgat acctcgatat ggggtatgtt atcgaagtag
    14161 atgcggctta acgccaccat cttcaggtac tcgtgcggcg gtacccggcg gatacggaac
    14221 ttcttggtct gtgcctggaa tgtccatggg acaaaagaaa gaataccacc ggtttcgtcc
    14281 tgcaggctac gcagtgccga caggtgtgcg atgatctcgg tatcttcttc ttcgctgccc
    14341 cagacaatgt tggtggagct tttcatgccc tgctgctgcg ccgtgcgcat tgcgcgcagc
    14401 cattctgcag tcgaagcttt cttgggtgaa agaatatcgc gcatacggtc ggtgagaatt
    14461 tcggcgcctg caccgggaaa actgttcatg ccggcatctg ccagttcgcg gaaaacttcg
    14521 gagagcggct tctgtgtcag tttttccatg aacagcactt cgacgggcga aaacgcgcgg
    14581 atgtgcactt cggggtattg gctgcgtacg gcagacagga tatcgaagta gtaatccatc
    14641 ggcagaagtg ggttaactcc cccctgtaaa aaaagctgcg gagcgtcgag cttgatggat
    14701 tcttcgactt tttttagtat ctcgtctttg ctcaggttat aacccttgcc agaacccact
    14761 tcgtccatga acgagcagaa gctgcattcg atattgcagt agttggtata gttgacgacg
    14821 cggaatgccg tgtaggtgac ctcgttcgct gggttcaggc ggttgcggat ttcgaccgcg
    14881 acgttaccta ggtcgcggaa gttggcttca cgccagagca gcagggcgtc tgcgtcgctc
    14941 agcctgttgc cggcgagtac tgagtcgagc actgcgctga ttttcttgtc catgcttggg
    15001 aatggttttt ttcggtgcaa caggccggaa aaggaaaaat caaaatacct gtccgttcat
    15061 gcccatgaat acctggaaac ggttaccccc ggcggcgaag aagggcacct cggcgcgggc
    15121 gaagagggca ctgcggctgc cgagcatgcc ctgcggcagg tcgaagatga gacccgcgcc
    15181 cgcaccggcg tggaagcgct gaccattgaa ctcgcggtca aaggtgactg catcggcgac
    15241 aaaccacagg ttcacggggc cgccggcatg cacgatcgca tcgagattca cgagcccgaa
    15301 tgcgggagcg cgaaattcag agaaagcata acccgcgacg cggcggtagc gcgcttcggg
    15361 gccaccgacg gaaaaagact gtatcacgtc ctggctttcg cctggcagag agaagttggt
    15421 ggctcccgcg cggccagcta tcatcaggcg cagcgctccg ccccagaact cgtcgccggc
    15481 acgtacgtgc agctcatgga tgatgacttc tttacgcgc
//
LOCUS       DAOQJF010000026        15406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1825_length_15406_cov_5.917595, whole genome shotgun sequence.
ACCESSION   DAOQJF010000026 DAOQJF010000000
VERSION     DAOQJF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_15406_cov_5.917595"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PLF85_02585"
     CDS             complement(<1..1266)
                     /locus_tag="PLF85_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNE18572.1"
                     /translation="MHLPYTLLLLLLYPLLRCAALFHNRLAENFALRSKLPDFSAARG
                     KLHVWFHAASAGEFEQARAVAQELRRLHKDIFLSFSFFSDSAYRAKKNDPLPDVLFAL
                     PLDFPWRMRKLVHAMQPNALVIAKYDAWPNQVRAAVKAGAKVYLVSATLPEKSLRWRF
                     PLKFLLRPVYGAMKTIFSINARHADRLRKISPDNVIVSGDTRFDAIALRLAADRTYSI
                     QIKRLQAELRGRQVLVAGSTYAESEKMLADYLGRRPRLSGKDVCALIAPHHVKPERIA
                     DLEENLRVNGLRSLRWSDWLNEQPARAKKISFDVLVIDALGVLPHLYPLAHVAYVGGG
                     FKGSVHSVIEPVIAGVPVITGPAIGNSAEAEELSAMGLLHVLPECSATQLWEAVENLG
                     RQRRSLSEGLKAYFRQRVGVSRQIVHTVMD"
     gene            complement(1266..1535)
                     /locus_tag="PLF85_02590"
     CDS             complement(1266..1535)
                     /locus_tag="PLF85_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNE18573.1"
                     /translation="MRRSYRVVGRVQGVGFRQFTAARAKELGLMGWVRNLPDGSVEAE
                     AQGHEVTLADFEALLRRGPQLSRVDSLVVAELPEQPAMTGFEILR"
     gene            complement(1537..2556)
                     /locus_tag="PLF85_02595"
     CDS             complement(1537..2556)
                     /locus_tag="PLF85_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HNE18574.1"
                     /translation="MKLFLSSLANIFKGDKKRHNTSTDLPDMGKQLTRADFMRIYDSM
                     PERARAQLFASLDYAQKGRLLDMLAGPQPPAAPEPASEPDWQAEDFLPEKDERKIERL
                     RHVREVKEVRAQFAEFARFSPAVVAEALGTETAALVAVVLLQLDHKFASRVLKALPEL
                     MRGEVVKAMASERRISGEALVALGKKIFQKIENMPEPESAKNDGIRHINEILKLMGAD
                     EAQRITRQVQESDADLADQLETTRYAFEDLVALSARDFRALFSEIPDEQLWARALKAT
                     DQMQRKQLLSKLPVKRAGMIAGAMSEIRTTRLESIDKARNQILKTALSLAAKHKISFA
                     GNGLH"
     gene            complement(2567..2893)
                     /locus_tag="PLF85_02600"
     CDS             complement(2567..2893)
                     /locus_tag="PLF85_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE18575.1"
                     /translation="MLRTKEVGNYIVAYLKGRLDVHISGEIEKDLMHLSEKNPDSDFI
                     LNLEGIEYMSSSGLRVLVALMRHLKGKSRKMKLCNMNVAVKKIFEVVELMDMFDIYQS
                     EEEALK"
     gene            complement(2895..3326)
                     /locus_tag="PLF85_02605"
     CDS             complement(2895..3326)
                     /locus_tag="PLF85_02605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE18576.1"
                     /translation="MSRVRKEFSADKAAIPLVHDFVRATLARNGVQGSQGDDIVLACD
                     EAATNIVEHAYDAARCTAQTRRSFVLALHCGKKRVIATFFDSGASFDIKGVEIPDIRR
                     NLTGEKRGGYGVFLMRRLVDKIVYSARRRLNVTRLVKEVSG"
     gene            complement(3323..5692)
                     /locus_tag="PLF85_02610"
     CDS             complement(3323..5692)
                     /locus_tag="PLF85_02610"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE18577.1"
                     /translation="METTPGLTAVGQASRILRNESGRLFIETNDELNKAAQYILKAAC
                     SLMHAEGCFLALKTQSGVRTYRISSRGLTALEKNIMQKSLAESETLLVVNKQQLYNRA
                     QESMGEAATSYIAAPLLTDGKQVLGALLWVTKLGEENFSEEDLSLASTFSRTFARMLA
                     NCIAAPDKTTLLVKFSSNLVTVFENLYLYRKNLENNFLLSEMIKVSKMINSTLDLNSL
                     LESIMDSAKIVLKAEASSLMLIDRKTNELFFNIISGEKERDLKEIRIPIGVGIAGIVA
                     DTCKPLVVNDAQNDERVYKQADEKINFVTRNLIATPLMVRNRIIGVIEVINSVGRTEF
                     SEKDLELFNTFSEQAALAIHNRELIDSLKDINRELKKKVHELSSLHEIGKVLMSTLNE
                     KDLFDSIVRIIADEMQADRVSIMIYAPDHDALEIISHYGLDLSPFERTFVPLDRSLAG
                     KSYRDLKLIYTNALEKSPYAAFRDIERYHTGGCIIQPLMHGQEIYGVLNISDRHAPTG
                     HEFTDDDLRLVSTIASQITRSIQNFRLLNEMLQKRSYEKELEITSSIQKSILPTHFQK
                     SAWFDMGVISEPAKLMGGDFYDFLAFDGDQFVFSVADVSGKSLPAALFMAVTSSIIRT
                     YGREVRKPAEVLHKANELIYQDSQSGMFVTLFYAYYDAPEHQLFYSSAGHNEQLVFRH
                     DTRTFEKLGTRGRPLGVVDSQLHGPFGEGKVFLSEGDLLILYTDGVVEAINERKEEFG
                     LDRFCRIISDNMLLSSDQLVKKIFREVKTFAGTEAQYDDFTLMIIKILK"
     gene            complement(5694..13268)
                     /locus_tag="PLF85_02615"
     CDS             complement(5694..13268)
                     /locus_tag="PLF85_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18578.1"
                     /translation="MKVLKRPGIASRIRFWGVLFFAAFFSAHCAGKDVRDDAKFSYQN
                     IYEDRYQRGNGGIIPITIERGESYSGSITNDQKYLYFSSNSSGNYDIYLRDLADVFSV
                     PVVSTVTNQKEPSISPDGKYLVYVDDELDPDGDIVLLKVNPKKLIELYRERKQPDEEW
                     FASKSKNLTNSEANRIRARDANPAWSPDGKYIAWSSDLDPAKADDLGAGAGALQNIWV
                     MPASDPEQKRQVTKRGGVMPAFSPDGARIVYVSYEDANSLGAVYEVELASGKTRRLTS
                     GHALDFYPSYAPDGNGIILTRIAADSNGDGQIDRKDAGQIIRIYPEAGGFVAADKHDT
                     DDLEITTVDDDEYVPLTSPSDHVFDSRVSNFIGGSVVLAQLKGEDINVAFIPLSGAIP
                     VKPDVRQQQQYLFDLQKRSKNQARICMALEQLPAAFGMAPDVVVYDAISSMRRARCDV
                     QALKDLQAFFATSDTAELALYRLLSDLSAFDPEYADLKGITSLEPLADTSNPEEYFEE
                     VLRQKKIWSYYRESEDPDDYRAVLAFVRHAEARYYIRQGKHKPAHEVISRILRENSAY
                     LALDELLYDNGVLDSSTLPANELVYLVASEPDNTVLKGYYNPGKKSAITIRPHIRRKA
                     EKFLLEFFERQFDAGTEEAQEQFLEAFPVKKNRTLHALFALAAARDNAENEIYDEAAN
                     DAARVQQLTEPASVFHYHAAIVLAGNAELKQGADAAIPLYTQAITGCRDTEAPENVGE
                     VITKIGVYYREKADQLRAAGDDRGATKQYEALLDLYLSAHANRLTKQLVSRDLLDYTL
                     NLDQIALRAATQDEDLLEDILSFYDSRIDLARRYLVTEFIFGRGFLRSQLGIQRHLAA
                     EAEGLSRSAKKEVFEYFRKAEIDLNWCFFANARFADAYIMLGWMYQFIDEKREIVLDA
                     ASGKRDREIFESLYKAYFPDYLFEKNIRLYQKTLALFGKSGSPRIRNSFHLNIANNYF
                     LLNNYSQAEEHFTAILDSKGNPDYQFESPEQEMMYYYHLGRTLYFTGKYDAAARYLRY
                     VENNLNARYPINGVSSTTQQLNQSRREIAYKTFALNSEYAQDTQASINYLQTIVAERQ
                     LAGAVTPGSMVHLELARLYLRNGDLSSSLEQTQKAEELLAKEDEIPIPKFKIRIKWFW
                     VYEPWTWLVGLIYKLPYDDIIIGDNHLAFELPTVNRYQLLYSIRADIYRSKGLLQEAS
                     GALAKLVEYAEKDKTKHGRETLSNAASRRAELEFSLRSWDSARKLYETALEQAEKNKN
                     AGAALMFRKNIELCRLRKLETALEPVADKIKTARRYADEIAEFEEDAVAERVKIARKA
                     VKEKEDPAKPDLTDQDIAKIRERTQTELQPVIFFRGLYLAHLAELEDFSGRVRDVQET
                     FDSYLSRKQATFNGYTSALKYFRGFTTEGFSEVHPAFEPDLKNNSLRIKLAMNRAKML
                     QEMSLFDESVAEFKAIQERSQEFRAQLEYAIASYRLYRVYEDAGLEDRLSTAPYNELV
                     RYFVEHPAFLKPNTDLFERLCNLLIDRAVRQRAYAEAMRLEDMKRQATALPLYFEDLK
                     LYGSKEETFGRLLTVEQTRHVLSERIRVARLQRQSPQQLEKDLLAFDAEAKKLRSRLV
                     EPDRLDYRYETLFSTGYSDAELFPLARHGFIYILKPRDELLLVYARAEATKKGLRWFY
                     ETREVQGEKPAVEQVKEFARKNRAAIIILSPHLLAEVPTDTELAQLALQTSLRSAINF
                     ARNPDLARRNVMQIVKVASFFSIGGKNDLDYQGVSSLQRVRNAKEAAASGIHSNVVDY
                     EAELARKAVVIDNALLTPAALFSLRSNPNYAIVSFQARDTLQKADEFRFAAAADLYFS
                     AMGAGQVLHTFSARARAKQAVETFLTAGTVTAGMLLTGNAQLPGYGDKDRNAAQQKTS
                     YLKKIAQARNQREYTEAASYAEDAISLLPKDASMRLLAAELYHIQGETESAQRQLSLV
                     EIDSRASLAEKLSYFRILLRTRGDAGAKEYLAAHPEVQRSVKKEPAEYQALRQLYAYS
                     SGELDLLQSPAAWEDSYSPRQIPRTIDDRIRNPELRNEICVAATASFEFSLVINSCMR
                     NTNADPTENSDRALIQSWFSGGKSELKAVVRSEDLEFNHAVSLLQSGYVADALPYIKK
                     TFGQLNLSVSENLLAVSLLRVLAQRKVKDAEMRVAASLLSDFAEKAATRTKNLQAKKF
                     YSLLARTEKVYKSGAAALDLPAKKGEATGVISAKNLILLMGMSIAPAAESLPEVATSS
                     FIADRNIEKDLRFLREAIESGSGEADCARDNCSLLIKYYLRQKQTMPAVDLVLRQQGA
                     GRSWEKLQNLPPGTYGYTELFTGEFYEWYFSGKEFTFDRLDEFNEKTVARPRTGRKYL
                     AWTPQKNLFSRLKISPPRESILVSAGQVDNTAPVAPKDVRIEAPQPATLYSALYAKWG
                     EGRKKNGAAVVQVKAPFDSAAATLSVYSEPVKLQDTVQMKPNGYHLFCDEGEGYNGFA
                     LFAQNMVENMIQRKMPPEAAYEAAGKALKGRQAVNRPRYYLYRN"
     gene            complement(13268..13999)
                     /locus_tag="PLF85_02620"
     CDS             complement(13268..13999)
                     /locus_tag="PLF85_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18579.1"
                     /translation="MKKSRKDLFRKAVADNLSQRSEENSELGDFVSDMKRSRNEGSDS
                     QPGEARQAAGSAEAKNSLPEQKEEKETGKAMESRLKRLTAGETQPAAFADDDEPPRWR
                     RFVVFGVVGLLVLLLGYWALFAPGSRPTVYLSNAPIEEALARNLRTANLTFPKNKTVY
                     LFFAAGGRIGQDKVVVKLTEIYADSSNMMKEETVAQIESSVRSSWRYFTTQFQKEYFD
                     HGGRYRVHVLAPNGEVIASQEFRIQ"
     gene            complement(13996..14784)
                     /locus_tag="PLF85_02625"
     CDS             complement(13996..14784)
                     /locus_tag="PLF85_02625"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE18580.1"
                     /translation="MSTVLSITNQKGGVAKTTTALALAHAFARRDEKRRVLLIDLDSQ
                     RNATSILLGGQDIPPERSVYALFQAKKPESSILYNTSLANLFVIPSNLHLVEVESQLT
                     GALDGFFRLNDAMQKLRHEFDFILLDCPPSLSVITINAMVAADFLLIPLQISKFSIDG
                     IQNIRDAVNTVNRRYNAHLKIAGGCLTLHDGRTTLSQAMLPEVEKILPILQTTVPRSV
                     AVEEAHLLRQDIHDYAPQNKVAVAYAALAEELEAVFAREQGGVS"
     gene            complement(14781..15401)
                     /gene="fsa"
                     /locus_tag="PLF85_02630"
     CDS             complement(14781..15401)
                     /gene="fsa"
                     /locus_tag="PLF85_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNE18581.1"
                     /translation="MAEIKAAHELGILDGVTTNPSLVAKTGKPFKTVIKEICSIVDGP
                     VSAEVVSTDKDGILKEGRELAAMAKNVVVKVPLIAEGLKAVRVLSSEGIKTNVTLCFS
                     ANQALLAAKAGATYISPFVGRLDDISTDGMELIEEIRTIYDNYEFETKILVASIRHPI
                     HLKQSALIGADVATIPFSVFNQLVKHPLTDRGLETFLADAKKFKWD"
BASE COUNT         3359 a         4161 c         4175 g         3711 t
ORIGIN      
        1 gtccatcaca gtatgcacaa tttgcctgct cacgcccact ctttgtctga agtaggcctt
       61 caaaccttcg ctcaacgagc gccgctgccg acccaggttt tccacagcct cccacaattg
      121 tgttgcactg cattccggca gaacgtggag caaccccatt gctgaaagct cttcggcctc
      181 ggcagaattg ccgatggcag ggccggtaat gaccggcacc ccggcaataa cgggttcgat
      241 cacagaatgc accgaaccct tgaacccgcc gccgacgtag gccacgtgcg cgagcggata
      301 aagatgcggc aggactccca gcgcgtcaat caccagcaca tcgaaggata ttttttttgc
      361 ccgcgctggt tgctcgttca gccaatcgct ccagcgcaat gatcgcagac cgttgacccg
      421 aagattctct tcgaggtcag caatccgctc aggttttaca tggtggggag cgattagggc
      481 gcagacatct tttcccgaaa gccgcggcct gcgtcccaga taatcggcga gcatcttctc
      541 tgactcagca taagtcgagc ccgcgacgag aacctgcctg ccgcgtaatt ccgcctggag
      601 tctcttgatc tggattgaat atgttcgatc agctgcgaga cgcagggcta tcgcatcaaa
      661 acgtgtatca cccgagacaa tgacgttgtc cggtgaaatt ttccgcaggc ggtctgcatg
      721 ccgggcattg atactgaaaa tagttttcat cgcgccatac acaggcctta gcagaaactt
      781 cagcgggaaa cgccaccgca gcgacttctc gggcagggtc gcgctcacca ggtaaacttt
      841 tgcgcccgct ttcaccgcgg cgcgtacctg gttgggccac gcatcgtact tggcaatgac
      901 cagggcattg ggctgcatgg cgtgcaccag cttacgcata cgccatggaa agtccagtgg
      961 cagggcaaaa agaacgtcag gcagcgggtc attctttttg gcccggtacg cgctgtcaga
     1021 aaagaaactg aatgaaagaa agatgtcttt atgcaggcgc ctgagttcct gcgcgacagc
     1081 acgagcctgc tcaaactccc ccgctgatgc agcatgaaac cagacatgca gcttaccgcg
     1141 cgccgcgctg aaatcaggca atttactgcg cagcgcgaaa ttttctgcca gcctattgtg
     1201 aaacagggca gcgcagcgca aaagcgggta caacagcaaa agtaataatg tataaggaag
     1261 gtgcatcagc ggagtatttc aaagcccgtc atcgctggct gctcgggcaa ttctgcgaca
     1321 acaagcgagt ctacacgtga aagttgcggc ccccggcgca ggagcgcttc aaaatctgca
     1381 agagtgacct catggccctg cgcttctgcc tcaacacttc catcgggaag attgcgcacc
     1441 cagcccataa ggcccagctc tttcgctcgc gccgcagtga actgcctgaa accgacgccc
     1501 tgcacgcggc ccacgacccg gtagctgcgg cgcatatcaa tgcagcccgt taccggcaaa
     1561 actgatcttg tgttttgcag ccagggaaag agccgtctta agaatctggt tgcgggcctt
     1621 gtcgatactt tcgagccgcg tcgtgcgtat ttcagacatg gcgccggcaa tcatgccagc
     1681 acgcttcacg ggcaatttgc ttaatagctg cttgcgctgc atttggtcgg tagccttcaa
     1741 tgcacgcgcc caaagctgtt cgtcgggtat ctcactgaaa agcgcacgaa aatcccgcgc
     1801 gctgagcgct accaggtctt cgaatgcgta gcgcgtggtt tcgagctgat cggcgagatc
     1861 tgcatcggac tcctggacct ggcgagtaat gcgctgtgcc tcatcggcgc ccatgagttt
     1921 aagaatttcg ttaatatgcc tgataccatc gttctttgct gactcaggtt caggcatatt
     1981 ctcaatcttc tggaatattt ttttacccag tgccaccagc gcctcgccgc tgatgcgccg
     2041 ctcagatgcc atggctttca caacttctcc gcgcatcaac tcgggaaggg ccttcagcac
     2101 gcgggaggca aatttatggt cgagctgcaa aagaacaact gccacgagcg cggctgtttc
     2161 tgtgcctaaa gcctcggcaa ccactgcagg cgaaaagcgc gcgaattctg cgaactgtgc
     2221 acgcacctct ttgacctcgc gcacgtggcg cagcctttca attttccgct cgtctttttc
     2281 cgggagaaaa tcttctgcct gccaatcggg ttcactggcc ggctcgggcg cagcaggggg
     2341 ttgcggccca gcaagcatat cgagcagccg gcctttctgc gcgtagtcga gcgatgcgaa
     2401 tagttgcgcg cgtgcacgct cgggcatcga gtcgtagatt cgcatgaaat ccgcccgggt
     2461 aagctgctta cccatgtcgg gcagatcggt ggaagtatta tgtctcttct tgtcgccctt
     2521 gaagatattg gccagagacg acaggaacaa tttcatcggg gcggagttat ttcagggctt
     2581 cttcttcaga ttggtagata tcgaacatgt ccatgagttc gactacttcg aagatctttt
     2641 ttaccgcaac attcatattg cagagcttca tcttacggct cttacctttg aggtggcgca
     2701 tcagggcaac caggacacgc agaccgctcg aagacatgta ttcgatgcct tcaagattga
     2761 gtataaaatc agaatcagga tttttttcac tgaggtgcat gaggtctttc tcgatttcac
     2821 cgctgatatg cacatccagg cgacctttaa ggtacgcgac gatgtagtta ccgacttcct
     2881 tggttctgag catgttaacc tgagacctct tttaccaacc tcgttacgtt gagtcgtcgc
     2941 cgtgcgctgt aaactatttt atcgacaagc cttcgcatga gaaaaacgcc atatccgccg
     3001 cgtttttcgc cggtcaggtt gcgccggatg tcaggaatct cgacgccttt tatatcaaat
     3061 gaggcccctg aatcaaaaaa agtcgcaata acgcgttttt tgccgcagtg cagggccagc
     3121 acaaaactgc gtcgcgtctg cgcagtgcag cgcgctgcgt catatgcgtg ttcgacaata
     3181 tttgtcgcag cttcgtcgca ggccagcaca atatcgtcgc cctggctgcc ctgcacgccg
     3241 ttacgcgcaa gcgtggcgcg cacaaagtca tgcaccaggg ggattgccgc cttgtcggcg
     3301 gagaactctt tacggacgcg gctcatttaa gtatctttat gatcatcagc gtgaaatcat
     3361 cgtattgcgc ttcggtaccg gcaaaggttt ttacctcacg aaagatcttc ttcacaagct
     3421 ggtctgaaga gagcagcatg ttgtcactga tgatacgaca gaacctgtcg agcccgaatt
     3481 cctctttccg ctcgttgatg gcctcgacaa cgccatccgt atagagaatc agcagatctc
     3541 cctcgctgag aaaaacctta ccttcgccga atgggccatg tagctgcgag tcaactaccc
     3601 ccagcggccg accccgtgta ccgagcttct caaaggtgcg cgtgtcatgc cgaaacacca
     3661 gctgttcgtt atgtcctgcg gaagaataaa atagctggtg ttccggggca tcgtagtaag
     3721 catagaagag tgtaacgaac atgcctgact ggctgtcctg gtagataagt tcattcgcct
     3781 tgtgcagcac ttctgccggc ttgcgcacct cgcggccgta tgtccgtatg atagagcttg
     3841 tgacggccat gaagagcgcc gcgggcagac tctttccgct gacatcggcc accgaaaaga
     3901 cgaactggtc gccgtcaaac gcgagaaaat cgtaaaaatc cccacccatc agcttcgcgg
     3961 gttcagagat aacgcccatg tcaaaccagg cagacttctg gaagtgcgtt ggcagaatcg
     4021 atttctggat gctcgaagta atttcgagtt ccttttcgta cgaccgcttc tgcagcattt
     4081 cgttcagcag cctgaagttc tgtatgctcc gcgtaatctg gctggcaatg gttgaaacga
     4141 gccgcaaatc gtcgtcggta aactcgtgtc ccgtcggcgc gtgtcgatcg gagatattga
     4201 gcaccccata aatttcctgg ccatgcatca gcggctgtat aatgcaaccg ccggtatggt
     4261 aacgttctat atccctaaag gctgcatagg gcgacttttc gagtgcattg gtgtaaatca
     4321 gcttgaggtc ccggtatgac ttacctgcca gggaacgatc gagcggcaca aacgtgcgtt
     4381 cgaagggcga taggtcaaga ccgtaatgcg atataatttc gagcgcatcg tggtcggggg
     4441 cgtagatcat gatcgaaacc cggtcagcct gcatctcatc ggcgatgatg cgcacaatag
     4501 aatcaaacag gtccttttcg ttcagcgtcg acatcagcac cttgccgatc tcgtgcaggc
     4561 tggagagttc gtgtactttc ttttttaact cgcggttgat gtctttcagt gaatcgatga
     4621 gttcgcggtt atggatcgcc agcgctgcct gctcggaaaa cgtattaaaa agttccaggt
     4681 ctttttcaga aaactctgtg cggccaactg aattgataac ttcgataacg ccaatgatgc
     4741 gattgcgcac catcaatggc gtcgcgatca ggttacgggt aacaaagttg attttttcgt
     4801 cggcctgctt gtagacacgc tcgtcgttct gcgcatcatt gaccaccagt ggcttacagg
     4861 tatcggccac gattcccgcg atacccacgc cgataggtat tcgaatctct tttagatcgc
     4921 gctccttttc tccggaaata atattgaaga agagctcatt cgtcttgcgg tcaatgagca
     4981 tcagggacga agcttcggct tttaaaacga tttttgccga atccatgatc gactcgagaa
     5041 gactgttcag gtcaagcgtc gaattgatca ttttcgacac tttgatcatt tcagagagaa
     5101 gaaagttgtt ctcgaggttc ttccggtaga ggtagaggtt ctcaaagacg gtaaccaggt
     5161 tactgctgaa tttcaccagc agggtcgtct tgtcgggcgc agcgatacag ttcgccagca
     5221 tacgtgcgaa agtgcggctg aacgtggaag ccaggctcag atcttcttca gagaaattct
     5281 cttcgccgag tttagtcacc cagagcaggg cacccagaac ctgtttcccg tcggtaagaa
     5341 gcggagcggc tatgtacgaa gtggcggctt cacccatgga ctcttgcgcc cggttataga
     5401 gctgctgctt attaacaacg agtaacgtct cactctcggc aagactcttc tgcatgatgt
     5461 ttttttcgag agctgtgagt ccacgactcg agatacggta tgtccggacg ccgctctgtg
     5521 tcttgagcgc aagaaaacac ccctcggcat gcatcagcga gcatgctgct ttgagaatat
     5581 actgcgccgc tttattcagt tcatcgttgg tttctataaa aaggcggccg ctttcattac
     5641 gcaagatgcg cgacgcttgt ccaacggcag ttaaccctgg tgtggtttcc attctagttc
     5701 ctgtacaggt agtaccgcgg gcggttcact gcctgcctgc ccttgagtgc cttgccggct
     5761 gcctcgtacg ctgcttcagg aggcattttt cgctgaatca tattttcaac catattctgc
     5821 gcaaacaagg caaaaccatt ataaccctca ccctcatcgc aaaacaggtg gtaaccgttc
     5881 ggtttcattt gaacagtatc ctggagcttc acaggttcac tatagacact gagcgtcgcg
     5941 gcagcagaat caaaaggagc cttgacctgg acgaccgctg ccccgttctt tttcctgcct
     6001 tcaccccatt tagcgtagag cgcagaatag agggtagctg gctggggagc ttcaattctc
     6061 acgtctttcg gcgctaccgg ggcggtatta tccacctgac ctgcagagac gagtatagat
     6121 tcgcgtggcg gagaaatttt tagcctcgag aacaagttct tctgcggtgt ccaggccagg
     6181 tactttctgc cagtacgcgg gcgggcaacg gtcttctcgt tgaattcatc cagccggtcg
     6241 aaagtgaact ccttgccgct gaaataccat tcatagaatt cgccggtaaa aagttcggtg
     6301 taaccgtagg ttcctggcgg caggttctgc agtttctccc atgaccggcc agcaccttgc
     6361 tgcctcagca ccaaatctac cgcgggcatg gtttgcttct ggcgaaggta atacttgatg
     6421 agcagcgaac agttgtcgcg ggcgcaatcg gcctcgcctg atcccgattc aatcgcttcg
     6481 cgcaggaacc gaagatcttt ctcaatatta cgatctgcga taaatgaact cgtagcaacc
     6541 tcgggtaaag actcggcagc aggggcaatg ctcattccca tcagcaggat gagattcttt
     6601 gctgaaatga ctcccgtcgc ttcgcctttt ttagccggca aatcgagcgc agccgcaccg
     6661 ctcttgtaca ctttctctgt gcgcgcgagc aggctataga atttttttgc ctggagattc
     6721 tttgtcctgg tggcagcttt ttctgcaaag tcgctcagca gcgaagctgc gacacgcatt
     6781 tcggcgtctt taaccttgcg ctgggcaaga acacggagca gcgagacagc gagcaggttt
     6841 tctgacacag aaagattcaa ctgcccgaag gtttttttta tgtaaggcaa tgcatcagcg
     6901 acgtaaccgc tctgcaacaa agatactgca tgattaaatt cgagatcttc gctgcggacc
     6961 actgccttga gctcagattt gcccccgctg aaccagctct ggatgagcgc acggtcactg
     7021 ttttctgtcg gatccgcatt ggtattgcgc atgcaggaat tgataacgag tgagaattcg
     7081 aacgaggctg ttgcggccac gcagatttca ttacggagtt cagggttgcg gattctgtcg
     7141 tcgatggtgc gaggtatttg gcgcggcgaa tagctgtctt cccatgcagc agggctctgc
     7201 agtaggtcaa gttcaccaga actgtatgcg tacagctggc ggagcgcctg gtactctgct
     7261 ggttcttttt tgacagagcg ctgtacttcg ggatgcgctg cgaggtactc ttttgcgcct
     7321 gcatcaccac gcgtgcgcag caagatacgg aagtagctga gtttctctgc caatgaagcc
     7381 cgggaatcta tttcaacaag cgatagctgt cgctgcgcag attctgtttc accctggatg
     7441 tgatagagct ctgctgccag caggcgcatc gatgcgtctt tcggcaacag cgatattgcg
     7501 tcttcggcat aagaggctgc ttcggtgtat tcgcgctgat tgcgtgcctg cgcaatcttc
     7561 ttcaggtaag aagttttctg ctgcgcagcg ttcctgtctt tatcgccgta accaggcaac
     7621 tgggcgtttc cggtcagtag catgccggcg gttacagtgc cggccgtcag gaatgtttca
     7681 accgcctgtt tggcgcgcgc gcgggctgag aaagtgtgca atacctgccc tgcacccatc
     7741 gcagaaaaat agaggtctgc cgcagcggca aaccggaact cgtctgcctt ctgcagcgta
     7801 tcacgcgcct ggaaagacac aatcgcgtaa ttgggattac tgcgcagtga aaagagggca
     7861 gcaggcgtga gaagtgcatt gtcaatgacg actgccttgc gggcgagctc cgcttcgtaa
     7921 tcgaccacat tgctgtggat accagatgct gccgcctctt ttgcattccg cacacgctgc
     7981 agggaactca caccctggta atccaggtcg ttcttgccgc caatactgaa gaaactggca
     8041 accttgacaa tctgcatcac attgcggcgc gccagatcag ggttacgggc aaagttgatt
     8101 gcagaacgca acgaggtctg caatgccagc tgtgccaact cggtatctgt cggtacctct
     8161 gccaaaagat gaggagagag aatgataatg gctgcgcgat tcttccttgc gaattctttc
     8221 acctgctcta ctgctggctt ctcaccttga acttccctgg tttcatagaa ccagcgcaac
     8281 cccttcttcg ttgcttcagc ccgggcatag accagaagca gttcatcacg gggtttgaga
     8341 atgtagataa agccatggcg ggcaagagga aaaagttcag cgtcagagta tccggttgaa
     8401 aagagagtct cgtagcggta atcaagcctg tctggctcta ccagacgact gcgcagcttt
     8461 tttgcctcgg cgtcaaaagc cagcaggtct ttttcaagct gctgcggcga ttgccgttgc
     8521 agtcgcgcaa cacgtatgcg ctccgacagg acatgccggg tctgttcgac ggtcagcagc
     8581 cggccaaatg tttcttcttt agacccgtag agtttcaggt cttcaaaata aagggggagc
     8641 gcggtagcct ggcgcttcat atcttcgagg cgcatggctt ctgcataagc gcgctgccgg
     8701 acggcacggt cgatgagcag gttgcagagc cgctcaaaga gatcagtatt gggtttcagg
     8761 aaagccgggt gttcgacaaa ataccgaacc agttcattat aaggggcagt cgacaggcgg
     8821 tcttcaaggc cggcgtcttc ataaacgcgg tagaggcggt aagatgcaat ggcatattcg
     8881 agctgcgccc ggaattcctg gctgcgttcc tgaattgcct taaattcggc aacggattca
     8941 tcaaacaggc tcatctcctg cagcatcttt gcgcggttca tcgcaagctt gatgcgcagg
     9001 gaattattct tcaggtcggg ttcaaacgca gggtggactt cggaaaaacc ctcggttgta
     9061 aaaccccgga aatatttcag cgccgaagtg tagccgttga atgtcgcctg tttgcgtgac
     9121 aggtaagagt cgaaagtctc ctgcacgtca cgcacgcgcc cgctgaagtc ttcaagctca
     9181 gccaggtgag ccaggtacag cccgcggaaa aagattaccg gttgtaattc agtttgtgta
     9241 cgctcacgga tttttgcaat atcctggtcg gtaaggtccg gcttggcagg atcttctttc
     9301 tctttgactg ccttgcgggc gatcttcaca cgttcggcga cggcatcttc ttcaaactcg
     9361 gctatttcat cggcataacg ccgggcagtt ttgattttgt ccgcaaccgg ttccagcgcg
     9421 gtctcgagct tgcgcagccg gcacaactcg atgttcttgc ggaacatgag cgcagcccct
     9481 gcattcttgt tcttttcagc ctgctcgagc gcggtttcat acagcttacg cgcagaatcc
     9541 catgaccgca gagagaattc cagctcagcg cgccggctgg cagcattgct cagggtctcg
     9601 cgaccatgtt tcgtcttatc tttctcagca tattcgacga gttttgccag tgcacctgat
     9661 gcttcctgca gcagcccctt gctgcggtaa atatcggcac gaatcgaata cagcaactgg
     9721 taacggttca ccgtcggcag ctcgaacgcc aggtggttat cgccaatgat aatgtcatcg
     9781 taaggtagct tgtagatcag gccgacgagc caggtccagg gctcgtacac ccagaaccac
     9841 ttgatgcgta tcttgaattt tggaatgggg atttcatctt ctttcgccag cagctcttcg
     9901 gccttttgtg tctgctcgag cgatgaactc aaatcgccat tacgcaggta aagccgggca
     9961 agctcaaggt gaaccattga gccgggggtc acagcccccg caagctggcg ttcagcgacg
    10021 atagtttgca ggtaattaat cgacgcctgc gtatcctgag catattcgct gtttagtgca
    10081 aaggtcttgt acgcaatttc acgacgcgac tggttgagtt gctgcgtcgt cgaactgacc
    10141 ccgtttatcg ggtagcgcgc attcaggtta ttttccacgt agcgcaggta acgcgcggca
    10201 gcatcgtatt tgccagtgaa atacagcgtc cggccgaggt ggtaatagta catcatctcc
    10261 tgttcgggag attcaaactg gtaatccggg ttacccttgc tgtcaagtat cgctgtaaag
    10321 tgctcttctg cctggctgta gttattgagc aggaagtaat tgttcgcgat attcaggtga
    10381 aagctgttac ggatgcgcgg cgaacccgac ttgccaaaaa gcgccagtgt tttttggtac
    10441 aggcggatat ttttttcaaa caggtaatcg ggaaagtagg ctttataaag cgattcaaat
    10501 atttcacggt cgcgtttacc cgaagccgca tcgagcacaa tctcgcgttt ttcgtcgatg
    10561 aactggtaca tccagccgag catgatgtag gcatcggcaa agcgcgcatt cgcgaaaaag
    10621 caccagttca ggtcgatctc ggctttgcga aaatactcga acacttcttt cttagcagag
    10681 cgcgagagac cctcagcctc tgcggcaagg tgccgctgga tacccagctg cgagcgcaga
    10741 aaaccccgac caaagataaa ctccgtcacg aggtaacgcc gcgcgagatc gatgcgtgag
    10801 tcgtaaaagg acaggatatc ttcgagcagg tcttcatcct gtgtcgcagc gcgcagcgca
    10861 atctggtcga ggttcagggt gtagtcgagc aggtcgcgcg aaaccagctg tttggtcagc
    10921 ctgttcgcgt gtgccgacag gtagagatcg agcagcgctt cgtattgttt ggttgcaccg
    10981 cggtcgtcgc ccgcagcgcg cagctggtca gctttctcgc ggtaatatac accaattttt
    11041 gtaatgacct caccgacatt ctccggcgct tctgtatcgc ggcagcctgt aatggcctgg
    11101 gtatagagcg gtatggctgc atctgcgccc tgcttgagtt cggcattacc ggcaaggaca
    11161 atcgctgcat ggtaatggaa taccgaagcc ggctctgtga gctgctgcac gcgcgctgca
    11221 tcgttcgctg cttcgtcgta gatttcgttt tcggcgttgt cgcgcgccgc agccagagca
    11281 aagagcgcgt gcagagtgcg gttctttttt acgggaaaag cttcgagaaa ctgttcctgc
    11341 gcttcttcag tgcccgcatc gaactgcctt tcaaagaatt cgagcagaaa cttttccgcc
    11401 ttgcgccgaa tatgcggccg aattgttata gcggattttt ttccgggatt gtagtatcct
    11461 tttaagacag tgttatccgg ttcgctggca acgaggtaga ccagctcatt ggccggcagt
    11521 gtagatgaat cgagcacgcc gttatcgtag agcagctcat ccagcgcgag gtaggcggag
    11581 ttttcgcgca ggatgcggct gatcacctca tgggctggtt tgtgctttcc ctgccgaatg
    11641 taataccgcg cttcggcatg gcgcacgaac gcgagcacag cgcggtaatc atcgggatct
    11701 tcactctcac gataataaga ccagatcttt ttctggcgca aaacttcttc gaagtactct
    11761 tcagggttgc tcgtgtcagc gagcggctcg agagaggtta tacccttgag gtcggcatat
    11821 tctggatcaa aagcagaaag atcgcttagc aggcgataga gcgcgagctc agccgtatct
    11881 gaggtggcga aaaatgcctg cagatctttc agcgcctgta cgtcgcagcg tgcccggcgc
    11941 atcgagctga tcgcatcgta gaccacaaca tcgggcgcca taccaaatgc cgccggcagc
    12001 tgctcgagag ccatgcagat gcgtgcctgg tttttgctgc gcttctgcaa atcaaagagg
    12061 tactgctgct gctgccggac atcgggcttt acgggtatcg cgcctgatag gggaatgaag
    12121 gcaacattga tatcttcgcc cttgagctgc gccaggacga cgctacctcc gatgaaattg
    12181 ctcacccggg aatcgaacac atggtccgag ggcgaggtca gcggtacgta ttcatcatcg
    12241 tcgaccgtgg tgatttcgag atcatcggtg tcgtgcttgt ctgctgcgac aaaaccacct
    12301 gcttcagggt agatgcgtat aatctgcccg gcatctttgc ggtcaatctg tccatcgccg
    12361 ttgctatcgg cggcaatacg cgtcagtatt atgccgttac cgtcaggtgc ataactcggg
    12421 taaaaatcga gagcatgccc cgaagtgagg cgccgggttt ttccgctggc aagctccacc
    12481 tcgtaaaccg cacccagcga attcgcatct tcatacgaga catagacgat gcgcgcacca
    12541 tcgggcgaaa atgccggcat gacaccgccg cgcttcgtca cctgccgctt ctgttcaggg
    12601 tcagatgccg gcatgaccca gatattctgc agagcgccgg cccccgcgcc caggtcgtct
    12661 gcttttgcag ggtcgaggtc agagctccac gcgatgtatt taccgtcggg cgaccacgca
    12721 gggttggcat cgcgcgcacg aatgcggttt gcctcgctgt ttgtcaggtt cttcgacttc
    12781 gatgcaaacc actcttcatc gggttgtttg cgttcacggt agagctcgat cagctttttg
    12841 ggatttacct tgagtaaaac aatgtccccg tcggggtcga gctcgtcgtc gacatagacc
    12901 aggtacttgc cgtcggggct gatcgacggc tctttctggt tagtcaccgt agagactacc
    12961 ggtaccgaaa aaacatccgc caggtcgcgc aggtagatat cgtaattgcc cgagctgttc
    13021 gaagaaaagt agaggtactt ctggtcgttg gtgatcgaac cgctgtacga ttcaccgcgt
    13081 tcgatggtga tgggaatgat gccgccgttt ccgcgctggt aacggtcttc gtaaatattc
    13141 tggtaactga acttcgcgtc gtcgcggaca tctttacccg cgcagtgggc gctgaaaaaa
    13201 gccgcgaaaa atagtacgcc ccaaaagcgg atacggctgg caataccggg tcgcttcaga
    13261 accttcatta ctggatgcga aattcctgcg acgcgatcac ctctccgttg ggagccagca
    13321 catgcacgcg gtatcggccg ccatgatcaa agtattcttt ctggaactgc gtggtaaaat
    13381 agcgccagct cgaccgcaca ctcgattcaa tctgcgcaac agtctcttct ttcatcatgt
    13441 tgctcgagtc agcgtagatc tcggtcagct tcacgaccac tttatcctgg ccgatgcggc
    13501 cgcccgcggc aaagaacagg taaacggtct tgttcttggg aaaagtcagg tttgcggtgc
    13561 gcaggttgcg cgccagcgcc tcttctatcg gggcattcga caggtagaca gtcggccgtg
    13621 aaccgggagc aaacaaggcc cagtagccga gcagcagcac cagcaacccg acgacgccga
    13681 acacgacaaa gcggcgccag cggggcggtt cgtcgtcgtc ggcaaacgcc gcaggctgtg
    13741 tttcgcctgc cgtcagtctt ttcaatcggc tttccattgc tttgccagtc tctttttctt
    13801 ctttttgttc cggcaagcta tttttggctt cggcgcttcc ggcagcctgc ctggcttcgc
    13861 cgggttgcga gtcactaccc tcgttgcggg agcgtttcat gtcactgaca aaatccccca
    13921 gttcagagtt ttcttcgctg cgctgcgaga ggttatcagc aaccgccttg cgaaacaggt
    13981 ctttgcggga ttttttcacg atacgccccc ttgctcgcgg gcaaatactg cctcgagctc
    14041 ttctgcaagc gcggcgtagg cgacggcgac cttgttctgc ggcgcgtagt cgtggatatc
    14101 ctgccgtaac aggtgggctt cttcgacggc gaccgacctt ggcacggtgg tctgcagtat
    14161 cggcagaatc ttctctacct cgggcagcat tgcctgcgac agcgtggttc ggccgtcgtg
    14221 cagggtgagg caaccgccgg caatcttcag gtgcgcattg tagcgccggt taaccgtgtt
    14281 caccgcgtcg cgtatgttct gtatgccatc gatcgaaaat tttgaaatct gcagcggtat
    14341 cagcagaaag tcagccgcga ccatcgcgtt gatcgtaatc accgaaagcg aaggcgggca
    14401 atcgagtaaa atgaaatcga actcatgccg cagcttctgc attgcgtcgt tcagacgaaa
    14461 aaagccgtcg agcgcgccgg tgagctgcga ctccacttcg acgaggtgga ggttagaagg
    14521 gataacaaaa agattcgcga ggctggtatt gtacagtatt gagctttccg gctttttcgc
    14581 ctggaagagt gcatagaccg aacgttcagg gggaatatcc tgcccaccga gcagtatcga
    14641 cgtggcgttg cgctgcgagt cgaggtcaat cagcagcacg cgccgctttt catcgcggcg
    14701 ggcaaaggcg tgcgcaagag ccagagccgt cgtggtcttc gctactccgc ctttttggtt
    14761 ggtgatggaa agtactgtgc tcaatcccac ttaaactttt tcgcatcagc gagaaaggtt
    14821 tcgagaccgc ggtcggtcag cgggtgcttc accagctggt tgaacacgct gaacggaatc
    14881 gtcgccacgt cggcaccgat aagagccgac tgcttcaagt gaatcgggtg gcggatactc
    14941 gccaccagga tcttggtttc aaattcgtag ttgtcgtaga tcgtgcgtat ctcttcgatc
    15001 agctccatgc cgtcggtgct gatgtcatcg aggcggccga caaagggcga tatgtaggtt
    15061 gcgccggctt ttgcagcgag cagcgcctgg ttggccgaga agcagagcgt aacattcgtc
    15121 ttgatacctt cagagctgag aacacggaca gctttcaggc cttcagcgat gaggggcacc
    15181 ttgaccacaa cattcttggc catcgcggcg agttcgcggc cttctttcag aataccgtcc
    15241 ttgtcggttg aaacaacctc tgcggaaacc ggcccatcaa cgatcgagca gatttctttg
    15301 atcaccgttt tgaaaggttt gccggttttt gccaccagag aagggtttgt tgtgacgccg
    15361 tcgagaatac ccagttcatg cgcggccttg atttcggcca catcgg
//
LOCUS       DAOQJF010000027        15252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1853_length_15252_cov_4.991446, whole genome shotgun sequence.
ACCESSION   DAOQJF010000027 DAOQJF010000000
VERSION     DAOQJF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 15252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15252
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_15252_cov_4.991446"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /gene="lpxI"
                     /locus_tag="PLF85_02635"
     CDS             <1..761
                     /gene="lpxI"
                     /locus_tag="PLF85_02635"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNE18582.1"
                     /translation="ETAFSRKIDFVVYHIVEADLDRELAADQRMEKRSVSLGNISQTI
                     QYLQHDKITQVILLGKVEKQRLLQDVARDAAADEIYNRAADRRDDTLFLQFAALLRAL
                     GIRILKQKELLPSCFLPRGIHTKKKPETKALKEDIEFGYELSRKVGSLDIGQTAVVFE
                     KMILAVEAIEGTDAAIRRAGEIAHKKGGVVCKTAKTSQDPRFDLPAVGLRTLETMAES
                     GMQALVIEAAHTLVVSPAEFMARADELGLIVVAQ"
     gene            841..2283
                     /locus_tag="PLF85_02640"
     CDS             841..2283
                     /locus_tag="PLF85_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18583.1"
                     /translation="MSHFAKYALLLCLAGTLLPGQTGSLWAAGKQNKENSDLARVGIA
                     RFANADQDADYEWVETSLPEAISNSMHARFEFISQDQNLVDSAFRTHYQQSDRIAVPT
                     AAKIASASQTDILIIGKFKVDRANSEIIMQAVIYNTQGGRFIGQVEERSEVNNRIFKQ
                     IDTMASSIVSVIYQYALQANKGSSYNNLRMLVLVPSFSTKDERAQAEAELLVLKDELA
                     RQTPGNYMTLFEFFERYNVAEAEQENALQLAANKDRSRIKIWLEHYGVTDAYLVFVSD
                     NKVNITAVSATKTAQVSYAVGATTEEKQKALQKAQTEVGSKTALLKDDGPFPGILNLH
                     VGVGAAKGILESGNRIGVMSGPVVHASVRLWRYFQPQFRFEGYYAFQQGELAGMLGAS
                     AFGGLGYTTGTNRWSITPFAAGGIFTARVKTDFDQIYVLLPSVSAGFLFTWNIRQGWG
                     VSFSASSQYVHDPIAPALFFTGTLASVVRF"
     gene            2452..3084
                     /locus_tag="PLF85_02645"
     CDS             2452..3084
                     /locus_tag="PLF85_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE18584.1"
                     /translation="MPLTQARQAADISALFRSQGQRLLVNKGGVIFFEGQRYKGVYFL
                     SRGTLKWFRLDHTGNECVLKIYAAGEVAGLPPLFERNTVKHYVATLKAISDAELYFWS
                     ESDFHAFMRQHPEHLLTFNEQLCRTLKDLVEQTSAVSLKSVPARLYDYLEKLGARLTP
                     VRLPLRKHELAAALNTCPESLSRAFSRLLREGHILHENGNYRVHTDQARH"
     gene            3138..3587
                     /locus_tag="PLF85_02650"
     CDS             3138..3587
                     /locus_tag="PLF85_02650"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE18585.1"
                     /translation="MNFDAFGGRSWLPGGLIAIFPASKAAFGQKTGQNAMQYEKALSP
                     SGRRRKSVSAENIYLFKTEVLRALSDETRQGIIVLLGKHGSLCVNDLAAFFNVGRPTV
                     SHHLHVLREAKVVRSEKQGKEVHYSLNLRNIRRSIRSLDKVLASIES"
     gene            3640..4395
                     /locus_tag="PLF85_02655"
     CDS             3640..4395
                     /locus_tag="PLF85_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNE18586.1"
                     /translation="MNTLVLIKQVPDTEAKINVAAGKISEAGIKWTISPYDEIALEEA
                     IRMKEKSGGTVTALAVGDDSVQNALRSAFAMGADTAIHVKVDGYQMLDSFGIAKAIAG
                     AIEGADYKVVLAGRQGSDSDNGQVPLILATLKGWGCVSFAKKVEISGDKAVIECEADG
                     GEAVYEAALPVVITANAGLNEPRYPSLKGIMASKKKPIESKTANIAAETKIEVIGFEP
                     PPARPAGRTIDGADAAEKAKALVKALREEIKAV"
     gene            4420..5370
                     /locus_tag="PLF85_02660"
     CDS             4420..5370
                     /locus_tag="PLF85_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNE18587.1"
                     /translation="MAKILAVGEIAGGVLKKASRETTSVGKKLGGSVSTVLIGSGAAA
                     GAAEVAKHGADTVYVGESATYNGEAYAKAIADLVKSKGYEIVILPHSWIGRDISSRIA
                     TLLNTGVISDATEIAMDGDRVKIRKPVYSGKAFVSLKVKASPQVVTIRPNAHPIAEAA
                     GAGAVENITVDAGASKAKQVKFEAKGGTRVSLTEADIVVSGGRGIKGPENFPLIEQLA
                     DILGAGVGATRAVVDAGWCDHTLQIGQTGQTVSPNLYIAVGISGAIQHMAGMGSSKYI
                     VAINKDPDAPIFKVSTYGVVDDLFNVMPTLKDELKAALGK"
     gene            5421..6782
                     /locus_tag="PLF85_02665"
     CDS             5421..6782
                     /locus_tag="PLF85_02665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNE18588.1"
                     /translation="MANKVKFDRDGDRIVIAGAIRTPVGQAGSSLSGKHAFELGSMVV
                     DELLKRTKLDKSKIDGVVAGEIGQSSKAPNAARVISVKNDLPLNASAVTVANNCVSGY
                     EAVFEAARRIIMGENDVVVVIGEESMSNFPIYLDNVKRNSKTATVEKLKANWAEVPNM
                     TDIQLIDGVAEGLNDPIRDAMMFATAEIVAQKLNLTREDLDKYAHGSYKKAYDALTAG
                     KYDKYLLPVKYGEKPDEVLDKDEFVMGKTGFIEKPERFAKSPVIYEKMPGGLKGLYDR
                     FEKWIGKKYEETAKPVVTLFNACPQSDGAGAVVMTTESKAKELGLPILAKVKGWGYAG
                     VDPAIMGLGIAYAMKQALENTGLKWEDISKYEIHEAFAATALGSMVVARDEFKYDLVS
                     RLEKGDVNPNGGTLAIGHPLGATGIRVLINQLLDIEAGANYAMGSICAGGGVGGALIL
                     EKP"
     gene            complement(6843..7643)
                     /locus_tag="PLF85_02670"
     CDS             complement(6843..7643)
                     /locus_tag="PLF85_02670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE18589.1"
                     /translation="MKKRIIALAMLASIYACSPKNGADVHLKADKIVYQQDGKDFEGF
                     LALPKKADKKLPAVLLIHDWTGLDDYEQMRAKMLAENGYVALAMDMYGKGIRAANHKE
                     AGELSGTYGKDRKLLRSRVATALQVLKARPEVDPARIAVVGYCFGGMAAIEAALMGAD
                     IRGVVTFHAALSFPTLIADAKNVKVPVLIHHGSADKFVPEKDVKALKSAFDKAAVKYE
                     LVVHEGATHGFTLKSNIGHEEHFGMKYDAKADLASWASMLSFLRENLK"
     gene            complement(7700..8794)
                     /locus_tag="PLF85_02675"
     CDS             complement(7700..8794)
                     /locus_tag="PLF85_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18590.1"
                     /translation="MLKSLEQIYGTRRSTSKYDGYVCTFDLDKTYLDTRFESLRKLVR
                     IPFEKAEEKKNIPGVAAVVRELRRGRDETHPPVPIYFISGSPEGMHKVVAEKLKLDGV
                     TFDGILFKNWRAAVKKLQFKKIIDKIGFKLAALIYARSVFPLKAQELLFGDDSEYDAT
                     IYALYADIVSGALEDFEVLTILKKWEVAEDERELIAQNLKRLRESGYKPRDAVARIFI
                     HLEQQTPAHYHTTLSDKVVPTQNYFQTAVILYKMDAITRAGLFRVISDMIRNYRFGVT
                     EFTASTEDLLRRSLIDKGEARKLLKIVYKGNPLALPRRIISDLRSDFVRTIDTFSSIP
                     DTIMPPMRRESDRHLTLVERYLRFEPKRHV"
     gene            complement(8798..9787)
                     /locus_tag="PLF85_02680"
     CDS             complement(8798..9787)
                     /locus_tag="PLF85_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNE18591.1"
                     /translation="MIVDLFVVVVALALLFDFINGFHDAANSIATIVSTKVLSPVQAV
                     LWAGFFNFLAFYIFKLHVADTVAKTVDTSAITMYVIMAGLLAAITWNLITWYFGIPSS
                     SSHTLIGGFAGGAIAHGGFGLVKMKTIWPILAFIFLAPFIGMVVSYFNSIILLHICKR
                     SHPQHADKWFRRLQLVSSAAFSLGHGGNDAQKVMGIIGAAMIVTKKIPNMSQLPYWVV
                     ISCYAAIGAGTLFGGWRIVKTMGSKITKLTSFEGVASEAAGAATLFSTGALGIPVSTT
                     HTITGSIIGVGLAKRISAVRWGITVKLFWAWIITIPISALFAVAFYHVIHAFV"
     gene            complement(9784..10431)
                     /locus_tag="PLF85_02685"
     CDS             complement(9784..10431)
                     /locus_tag="PLF85_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNE18592.1"
                     /translation="MSLTSFFRFLSPKDQTFGPLFNEDTANLVQMGKSLNAMVRARKQ
                     KDRDQLSEEIDRLESQGDEITHQIFVALGKTFITPFDREDVHALAASLDDIADYMQGA
                     AARARLYNIVSFPPAIVDLVEILQQGIDEVVVAVSLVMTLKDRQKIREALVKIHSLEN
                     EADRIFNRAIEELFRKEKNAIQLIKMKELLATIETATDRCEDVANVIESIVIKYS"
     gene            10548..11150
                     /gene="hisB"
                     /locus_tag="PLF85_02690"
     CDS             10548..11150
                     /gene="hisB"
                     /locus_tag="PLF85_02690"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNE18593.1"
                     /translation="MARTAKIDRKTSETQIALSLNLDGNARMSGNNPIPFFEHMLGHI
                     VKYSMFDLELDLKGDIGIDCHHSVEDTAIVFGQALVQALGNKTGIFRYGSFTLPMDEV
                     LTTVTIDLSGRPYFVYRGESLKPMGKFGIYDSELTLEFLHKLSIYGAMNLHVQVHYGD
                     NRHHIHESIFKALGFALRQAVAIDARRANEVPSTKGSLTG"
     gene            11177..11800
                     /gene="hisH"
                     /locus_tag="PLF85_02695"
     CDS             11177..11800
                     /gene="hisH"
                     /locus_tag="PLF85_02695"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNE18594.1"
                     /translation="MSKKARIILLDFGMGNIRSLQKAFEHLGQSVTVSSDPKEAEAAD
                     VLLLPGDGAFARAMEELNTRNLVEPIYNAREKKKIIFGICIGFQLLFESSREFALANG
                     LGFLPGHITRLEKNTQAPVIPHMGWTTTEFSPESRLGKGIAPHSMFYYVHSYAFAGRH
                     KYATATTSYGQVFTSVIEHENLFAAQFHPEKSHTPGLALLRNFIELL"
     gene            11797..12534
                     /locus_tag="PLF85_02700"
     CDS             11797..12534
                     /locus_tag="PLF85_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HNE18595.1"
                     /translation="MKLIPALDILDGKVVRLKQGDYNAVTIYHEKPKDLAWYLADHGA
                     ERLHLVDLRGSKEGKICEGRLFTEIRRAVNIPCEAGGGIRSATDFRYYYDNGFEAGKD
                     FVMVGSLPFVDPPAFDAILAEFAGSLLMTVDAWGTQVKHSGWLKDSGVELDDFIAGLI
                     KEGVQNFLVTQIKKDGMMAGPDYEMYERLLAKFPQSRIIASGGVSSMDDLKKLATMKL
                     YGAIVGKAYYEGKITAEMLRNFRLSRG"
     gene            complement(12515..13306)
                     /locus_tag="PLF85_02705"
     CDS             complement(12515..13306)
                     /locus_tag="PLF85_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18596.1"
                     /translation="MSEKRFDFQDLEEIDFADELPAATHFSGREKLFLGTLSREELWQ
                     ALEDSHIIAAVRERGYWPISLETEFLTERDHRFYLLFRNEKILHMRLRLSFFRLHRHP
                     LMPAMKMLFIDWMQSRHVLRGATHTRELFPGKDVPGLGIFSRIDIFLRNLVVATHAFG
                     GFNIPEYFHDALLFHRAFRFYDPAREAFFRALMRDLRPHGAREISRALSEGRIRNRKN
                     EVQEWHGAEMLIFTHKPYAESIFDKRYDREVERKMREFSFSLAKA"
     gene            complement(13332..14219)
                     /locus_tag="PLF85_02710"
     CDS             complement(13332..14219)
                     /locus_tag="PLF85_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNE18597.1"
                     /translation="MRKKSLELVYSPVYDLTDYAHVLSAGKYRMLYDELKHSRWHWHT
                     PQRADEATLRLVHTERYLRDFLGAKLTDKTLRAEVPIDERIVDAVCTATQGTVLAAEL
                     ALKHGTVSNLSGGFHHAFADHAEGFCFLNDTAVAIKYLRQTRPGLKVAIIDLDVHQGN
                     GTAHILRKDEDCYTFSMHEKENYPKKEESSHDVHLASFLGDEEYLAILDENLAKLRQQ
                     FAPDLIFYIAGVDVYKDDMLGGLQLSFEGIAERDRRVRDFMPEVPLVVLPAGGYARNL
                     AHTVKLHAQTIEILHGRLN"
     gene            complement(14216..14536)
                     /gene="cutA"
                     /locus_tag="PLF85_02715"
     CDS             complement(14216..14536)
                     /gene="cutA"
                     /locus_tag="PLF85_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNE18598.1"
                     /translation="MSTSNEILIFTAINDSEMAQDLVTSLLEEGLIISGTLFPGTTLL
                     YKWENKINLDDETKIIIKAKRENYDRIEEYMMKNHPYKMPEMTSVDASFGSEKFREFC
                     RNKK"
     gene            14674..>15252
                     /locus_tag="PLF85_02720"
     CDS             14674..>15252
                     /locus_tag="PLF85_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18599.1"
                     /translation="MPASTANLGPGFDIWGIALDLRNDFICDRADRKDGSIKLSFLSG
                     DQTVVAPASLPRKVENDNLFVRAYNYLLKKAGQTPVAWDVKIIVNVPFSRGLGSSSTA
                     ILAGLTLANETLRRDYQMAYRIDQILDFALEFEPHPDNLTAALYGGWNLCMPLGGQTP
                     NIDAEKGASGPAFHRLPITVKAPVRIAGIIPDL"
BASE COUNT         3675 a         4155 c         4065 g         3357 t
ORIGIN      
        1 cagaaaccgc gttcagccgc aagatcgact ttgtcgtcta tcacattgtt gaagccgacc
       61 tggaccgtga gctggctgcc gatcagcgta tggaaaagcg cagtgtttca ctgggaaata
      121 tttcgcagac aatacagtac ttgcagcacg acaagatcac gcaggtaatt ctgttgggta
      181 aagtggaaaa acagcggctg ctgcaggacg tagcgcgcga cgctgccgcc gacgaaattt
      241 ataaccgcgc agcagatcgc cgtgacgaca cgcttttcct gcagttcgcc gcgctgctgc
      301 gcgcgcttgg catcagaatt ctcaaacaga aagagctttt gcccagctgc tttctgcccc
      361 gtggtattca cacgaaaaaa aagcccgaaa ccaaggccct taaagaggat atcgaatttg
      421 gttatgagct cagccgcaag gtaggcagcc tcgatatcgg gcaaacggcc gtggttttcg
      481 agaagatgat ccttgcggtc gaggccattg agggtacaga tgcagcgatc aggcgcgctg
      541 gggaaattgc gcacaagaaa ggcggcgtag tctgcaaaac cgctaaaaca tcccaggacc
      601 cccgcttcga cctcccggcc gtgggcctca ggacgctgga aaccatggcc gaatcgggca
      661 tgcaggcgct cgtgatcgag gcggcacaca cgctcgtggt cagccctgca gaattcatgg
      721 cacgcgccga cgagctgggc ctgatcgtcg tcgcacagtg aaggctgcct gccccagggc
      781 tcccgtgggg ctttttttgc tgtactgtgc gagtttattg cgtctaataa gctgacgcat
      841 atgtcacatt tcgcgaaata tgcccttttg ctctgcctcg cgggcacact gctgcccggg
      901 cagacaggta gcctttgggc agccggtaaa cagaacaaag aaaactctga ccttgcgcgc
      961 gtcggtattg cacgctttgc caatgccgac caggacgcag actatgaatg ggtggaaacc
     1021 agtctgcccg aggcaatcag caattcgatg catgcacgct tcgagtttat cagtcaggac
     1081 caaaacctcg tcgacagcgc cttccggacc cattaccagc agagcgaccg tatcgcagtg
     1141 cccaccgccg cgaaaattgc gtcagcttcc cagaccgaca tccttatcat tggcaagttc
     1201 aaggtagacc gcgccaattc agaaatcatc atgcaggctg ttatctacaa tacgcagggc
     1261 ggcagattca ttggccaggt ggaagagcgg agcgaagtca acaaccggat cttcaaacag
     1321 atcgatacga tggcatcatc cattgtttcg gtaatttacc agtatgcgct gcaggcaaac
     1381 aagggttcga gctacaataa cctgcgtatg cttgtcctgg taccgtcatt cagcacgaaa
     1441 gacgagaggg cgcaggcaga agccgagctg cttgtcctga aagatgaact cgcgcgccag
     1501 acaccgggca actacatgac tctgtttgaa ttctttgagc gctacaatgt agctgaagcc
     1561 gagcaggaaa atgcactgca actggctgcc aataaagatc gtagccgcat caaaatctgg
     1621 cttgaacact acggagtcac cgatgcctac ctggttttcg tgagtgacaa caaagtgaat
     1681 atcaccgccg tgtcggcaac caagaccgcg caggtttcat acgcggtggg tgcaacaaca
     1741 gaagaaaaac aaaaagctct tcagaaagcg cagaccgagg tgggcagcaa aaccgccctg
     1801 ctaaaagatg atggtccctt tcccggcata cttaacctgc acgtcggcgt cggcgcggcc
     1861 aaaggcatac tcgaatcagg taaccgcatt ggtgtgatga gcgggcccgt cgtgcatgct
     1921 tcggtcaggc tttggcggta ctttcagccg cagttccgtt tcgaaggtta ttatgcattc
     1981 cagcagggcg aactggccgg catgctggga gcttcagcgt tcggtgggct cggttacacg
     2041 acaggaacca accgctggtc gatcactccc tttgccgcag ggggaatatt taccgcgcgc
     2101 gtgaagacgg actttgacca aatttatgta cttttgccct cggtttcggc gggctttctc
     2161 tttacatgga acatacgcca gggttgggga gtcagcttca gcgccagcag ccagtacgtt
     2221 cacgatccga ttgccccggc actctttttt accggcacac tggcatcggt tgtgagattc
     2281 tagcaggttc cggaaaccgc acaaggctgc agccggtgcg ggagtttttc cggaacctgc
     2341 cagcccgcgc aagaaatcca ccagcctgcc gagctgcccg ggttatcccg ggaaatttgc
     2401 ttttcctttc atccataccc gtactattgc accgtgtctg agccaaaagc tatgcccctt
     2461 acacaagcca ggcaggcagc ggatatctca gctttgttca gaagccaggg ccaaaggctg
     2521 ctggtaaaca aaggcggagt gatattcttt gagggacagc gctacaaagg cgtctatttt
     2581 ctgagtcgcg gaacactgaa atggttccga ctcgatcata ccggcaacga atgcgtactc
     2641 aagatctacg ctgcgggcga agtcgccggc ctgccgccac tgtttgagcg caatacagta
     2701 aaacactatg tcgctacttt gaaggcaatc agcgatgcag agctttattt ctggtccgag
     2761 tcagattttc acgcgtttat gcggcagcac cctgagcacc tgctcacctt caacgagcaa
     2821 ttgtgccgga ccctcaagga tcttgtagag caaacctctg ccgtctcact gaagagcgta
     2881 cccgcccggc tatacgacta cctcgaaaaa ctcggggcaa gattgacgcc ggtcaggttg
     2941 cccctgcgca agcatgaact tgctgctgcc ctcaatacct gccccgaaag cctgagccgt
     3001 gcattcagca ggctcttgcg agaaggccat atattacatg aaaacggtaa ttaccgggtt
     3061 cataccgatc aggcccgaca ctaggctgcg ttttttcttt tcgccctgtt cgacttagcc
     3121 aaagtgacaa cgcgtcgatg aatttcgacg cgtttggagg ccgcagctgg ctgccgggtg
     3181 ggttaatagc cattttcccg gcttcaaaag cggctttcgg gcagaagacc gggcagaatg
     3241 caatgcagta cgagaaagcg ctttcacctt cgggacgcag gcgcaaatcc gtttctgcag
     3301 aaaacatcta cctcttcaag actgaggtct tgagggcgct gtccgatgaa acccggcagg
     3361 ggatcatcgt tttgctcggt aaacacggca gtctgtgcgt gaacgatttg gcggctttct
     3421 ttaacgtggg tcgcccgacg gtatcgcacc acctgcatgt gctgcgcgaa gccaaagtag
     3481 tacggtcgga gaaacagggg aaggaagtac actactccct gaacctgcgc aacatccgcc
     3541 ggtcgattcg aagcctggac aaagtcctgg caagtattga atcgtgaggg gctgcaaaga
     3601 acctttcact tttcaaataa gttttttaag gagtaacgga tgaacacact ggttttgatc
     3661 aaacaagtgc ctgacaccga ggcaaaaatt aacgtagccg ccggaaaaat ctctgaggcg
     3721 ggaatcaaat ggacaatctc accttacgat gagattgcac ttgaagaagc gattcgtatg
     3781 aaagaaaaat cgggtggaac cgttacagcg ctggctgtcg gcgatgactc ggtacagaac
     3841 gcactccgct cggcttttgc gatgggcgca gacacagcaa tccacgtgaa agtcgatgga
     3901 taccagatgc tcgacagctt cggcatcgcg aaagctattg ccggcgcgat agaaggcgca
     3961 gactacaaag ttgttctcgc cggccgccag ggttctgact ctgacaacgg acaagtaccg
     4021 cttatcctgg caacgctcaa aggctggggc tgcgtttcgt tcgcgaaaaa agtagaaatc
     4081 tcaggcgaca aagcggtcat cgaatgcgaa gccgacggcg gcgaagctgt ctatgaagct
     4141 gctctcccgg tcgtcatcac agcaaacgca ggtctcaacg aaccgcgcta cccatctctc
     4201 aagggtatca tggcatcaaa gaaaaagcca atcgaaagca agactgcaaa cattgccgca
     4261 gagactaaaa tcgaagttat cggttttgaa ccacctccgg cacgcccggc gggccgcact
     4321 atcgacggcg ctgacgccgc tgagaaagcc aaggcgcttg taaaagcgct gcgcgaagag
     4381 atcaaagctg tttaagcttt aaggagaaag gagaatacta tggcaaagat tctggcagtc
     4441 ggcgaaatcg ccggcggagt tctgaaaaag gcatcccgcg agacaaccag cgttggcaaa
     4501 aagctcggtg gttcggtttc aacagtcctg atcggttcag gcgctgcagc tggtgcagct
     4561 gaagtggcga aacacggcgc cgacactgta tacgttggtg aaagcgctac ctacaacggc
     4621 gaagcatatg caaaagcaat tgctgacctg gtgaagagca aaggttacga aatcgtaatc
     4681 cttccccact cgtggatagg ccgcgacatc tcttcgcgca tcgcaaccct gctgaacaca
     4741 ggcgtgattt ctgatgcaac ggaaatcgcg atggacggcg accgcgtgaa gatccgcaag
     4801 ccggtctatt caggtaaagc attcgtcagc ctcaaagtaa aagcatcccc acaggttgtg
     4861 actattcgcc ccaatgcaca tccgatcgct gaagcagcgg gtgcaggcgc ggtagaaaat
     4921 atcaccgtcg atgccggcgc atccaaagcg aaacaagtga agtttgaagc caaaggcggt
     4981 acacgcgttt cactgaccga agcggatatc gttgtatcag gtggccgcgg tatcaagggt
     5041 ccagaaaact tcccgctgat cgagcaactt gctgatatcc tcggcgcagg tgttggcgcg
     5101 acccgcgcgg tggtcgacgc tggctggtgc gaccataccc tgcagatcgg acaaactggt
     5161 cagacagtct ccccaaacct gtacatcgca gtaggtattt caggcgcaat ccagcacatg
     5221 gctggtatgg gctcatcgaa atacatcgtg gcgatcaaca aagatccgga tgctccgatc
     5281 tttaaggttt cgacctacgg cgtggtcgat gatcttttca acgtgatgcc aaccctcaaa
     5341 gacgagctca aggcagctct cggtaagtga tgtgaagggc tgccggtaaa accggcagcc
     5401 agattattta aggagaaaac atggcgaata aagtaaaatt tgaccgcgac ggcgatcgca
     5461 tcgttatcgc gggagcaatc agaactccag tcggccaggc aggctcaagc ctctcgggca
     5521 aacacgcttt cgaacttggt tcgatggtag tggacgagct gctcaagcgt accaaactcg
     5581 ataagagcaa gatcgacggt gtcgtcgcag gcgagattgg ccagtcatcg aaggccccta
     5641 acgcggcccg cgtgatctcg gtaaagaatg accttcctct gaacgcgagc gcggtgaccg
     5701 ttgccaacaa ctgcgtttca ggctatgaag cggtattcga agcagcgcgt cgtatcatca
     5761 tgggtgaaaa cgacgtggtt gtagtcatcg gcgaagagtc aatgtcaaac ttccccatct
     5821 acctcgataa cgtgaagcgc aacagcaaga ccgcgactgt tgaaaaactg aaagcaaact
     5881 gggcagaagt cccgaacatg actgacatcc agctgatcga cggcgtggca gaaggactga
     5941 acgacccaat acgggatgcg atgatgttcg caacggcaga aatagttgca cagaagctca
     6001 atctgacccg ggaagatctc gacaaatacg cgcacggctc atataaaaaa gcctacgacg
     6061 cactgacagc aggcaagtac gacaaatacc tgctgccggt aaaatacggt gagaagccag
     6121 acgaagtgct cgacaaagat gaattcgtca tgggcaaaac cggctttata gaaaagccag
     6181 agcgcttcgc gaaatcgcct gtaatctacg agaaaatgcc tggcggcctc aagggcctct
     6241 atgacaggtt cgaaaagtgg atcggcaaga agtacgaaga aactgcgaaa ccggttgtaa
     6301 cgctcttcaa tgcgtgcccg caatcagatg gggcaggtgc agttgtcatg accactgagt
     6361 caaaggcaaa agaacttggt ctgccaatcc tggcaaaagt caaaggctgg ggttatgcag
     6421 gggttgatcc tgcaatcatg ggcctcggca ttgcttacgc catgaagcag gctctcgaaa
     6481 acaccggcct gaagtgggaa gacatcagca aatacgaaat tcacgaagcg tttgcagcaa
     6541 ccgctcttgg ttcaatggtc gttgcacgtg atgagttcaa gtatgacctg gtttcacgcc
     6601 tggaaaaagg cgacgttaac ccgaacggtg gtacactcgc tatcggccat ccactgggtg
     6661 caaccggtat acgcgtgctg atcaaccagc tactggacat cgaagccggt gctaactacg
     6721 ctatgggttc gatctgcgct ggcggtggcg tcggcggagc tctgattctc gagaaaccgt
     6781 aacgctgaaa ggcggcttaa ccgcggtttt gaaaaagggt ggcacgtgcc gccctttttt
     6841 tgttacttga gattttctct caggaaagac aacatgcttg cccatgaagc aagatctgcc
     6901 ttagcatcgt acttcatgcc gaaatgctct tcgtgaccaa tgttggattt cagggtgaag
     6961 ccgtgagttg ctccctcgtg gacgactagt tcgtacttaa ctgcagcctt atcgaatgct
     7021 gatttcagcg ctttaacatc tttttcaggc acaaacttgt ctgcactacc atgatgaata
     7081 agcaccggca ccttgacatt cttcgcatct gcaatcagcg tcgggaacga tagcgccgca
     7141 tggaaagtaa ccacgcccct gatgtcagcg cccatgagtg cagcttcgat cgcggccatg
     7201 ccaccaaaac agtaacccac cacggcaatg cgtgcaggat caacttcggg acgtgctttc
     7261 aacacctgta gtgccgtggc aacgcgcgac ctgagcaact tgcggtcttt accgtaagta
     7321 ccgctaagct cccccgcttc cttgtggttc gctgcgcgga taccttttcc atacatatcc
     7381 atcgccagag cgacgtaacc gttttcggcc agcatttttg cccgcatttg ttcgtaatca
     7441 tccagcccgg tccagtcgtg aatgagaagg actgcaggca gcttcttgtc cgcttttttg
     7501 ggaagagcca ggaatccttc aaagtcctta ccatcctgct ggtagacaat tttgtcggcc
     7561 ttcaggtgca catctgcgcc attttttggc gagcaggcat agatagaagc cagcatcgcc
     7621 agagcgatta tccgtttttt catagtgcga caggcctaaa gccggccctg aattcgtcaa
     7681 acggtaatgc aggctatcgt tatacgtgcc gtttcggttc aaatctcagg taacgctcga
     7741 cgagcgtcag gtgccggtcg ctttcgcgac gcatgggggg cataatcgta tcaggaatgc
     7801 tgctgaatgt gtcgattgtc cgcacaaaat cgctgcgtag atctgagatg atgcggcgcg
     7861 gcaatgccag gggatttccc ttgtatacaa tcttgagcag cttacgcgcc tcgcccttgt
     7921 cgatcagaga gcggcgcagc aggtcttcgg tcgaagccgt aaattccgtc accccaaacc
     7981 ggtagttccg gatcatgtcc gaaatcacgc gaaacaaccc agccctggtg atagcgtcca
     8041 tcttgtagag aatcacagcg gtctgaaaat agttctgcgt cggtaccacc ttgtccgaca
     8101 gcgtcgtgtg gtaatgggcc ggcgtttgct gctccaggtg gataaaaata cgggccacag
     8161 cgtcgcgcgg cttatacccg ctttcacgca gccgcttcag gttctgcgca atcagctcgc
     8221 gttcgtcttc tgccacttcc cactttttca gaatggtcag tacttcaaaa tcttcaagcg
     8281 ccccggagac gatgtctgcg tagagcgcat aaatggtggc gtcatactct gaatcatcgc
     8341 caaacaatag ttcctgcgcc ttgaggggaa agacgctgcg cgcatagata agggcggcga
     8401 gcttgaagcc aattttatca attattttct tgaactgcag cttcttcaca gcggctcgcc
     8461 agtttttgaa caggatgccg tcgaaggtaa ctccatctag tttcagcttt tccgccacga
     8521 ccttgtgcat gccttcgggc gaaccggaaa taaaatagat cggcacaggc ggatgcgtct
     8581 catcccggcc ccgccgtagc tcgcgcacca cggcagcgac cccgggaata ttcttctttt
     8641 cttcagcctt ttcaaagggt atgcgcacaa gcttgcgcag cgactcaaac cgggtgtcga
     8701 ggtatgtctt gtcgaggtcg aaagtgcata cgtacccatc gtactttgag gtgctccgtc
     8761 gcgtcccgta aatctgttcg agcgacttga gcatctatca gacaaaagcg tggattacat
     8821 ggtagaacgc gaccgcaaac agtgcgctaa taggaatcgt aataatccag gcccagaaca
     8881 atttcacggt aataccccag cgcacggcgc tgattcgctt tgccagccct acaccgataa
     8941 tactgcctgt aatcgtgtgc gtcgtactga caggaattcc cagtgcgccg gtagaaaaca
     9001 gggttgcggc gccggcagcc tccgatgcta ctccctcaaa agacgtcagc ttcgtgatct
     9061 ttgagcccat cgttttcaca atgcgccagc cgccgaacaa cgtaccggcg ccaatcgcag
     9121 cataacacga aatcaccacc cagtacggca gttgcgacat gttggggatt ttcttcgtca
     9181 cgatcatcgc tgcaccaatg atacccatta ccttctgcgc atcgttaccg ccgtgcccga
     9241 gggaaaatgc cgccgaagaa accagctgca gacgcctgaa ccacttgtcg gcgtgctgcg
     9301 gatgcgagcg cttgcagata tgcagcagga tgatcgaatt gaaatacgat acgaccatgc
     9361 cgataaaggg cgcaagaaag atgaatgcca gaatcggcca gatggtcttc atctttacga
     9421 gtccaaaacc accgtgcgcg atggcaccgc cggcaaaacc gccaatcagc gtatgagacg
     9481 aactggaagg aatgccgaaa taccacgtta tcaggttcca ggttatcgct gcgagcaggc
     9541 cagccatgat tacgtacatg gtgatcgcgc tcgtgtctac tgtcttcgca actgtatcgg
     9601 ctacatgaag cttaaagata tagaatgcca aaaagttgaa aaatcccgcc cataagaccg
     9661 cctgtacggg ggacaagact ttcgtggaaa caatcgtggc aatcgagttg gcggcgtcgt
     9721 ggaaaccatt gataaaatca aagagcagcg ccagcgcaac gacaacaacg aacaggtcga
     9781 cgatcatgag tacttgataa cgatgctttc tatcacgttg gcgacatctt cgcagcgatc
     9841 agtcgcggtc tcaatggtcg ccagcagttc tttcatcttg atcagctgga tcgcattttt
     9901 ttccttacgg aacaactctt ctatcgcgcg gttgaagata cgatctgcct cattctcgag
     9961 actatgaatc ttcacgagcg cctcgcggat tttttgccgg tctttcaggg tcatcaccag
    10021 cgagacggcc acgaccacct cgtcgatgcc ctgctgcaga atctctacca ggtcaacgat
    10081 cgccgggggg aacgaaacga tattgtaaag gcgcgctctg gcggccgccc cctgcatata
    10141 atcggctatg tcgtccagcg atgctgccag ggcgtgcacg tcttcccggt cgaacggggt
    10201 aatgaacgtt tttcccaatg cgacaaagat ctggtgagtg atttcgtcac cctggctttc
    10261 gaggcggtca atctcttcgc tcaactggtc gcggtctttc tgtttgcgcg cccgaaccat
    10321 ggcgttcagg gattttccca tctgtaccag gttcgccgta tcttcattaa aaagcggtcc
    10381 gaaagtctgg tccttggggg ataaaaaacg aaaaaaggat gtcagtgaca tagaaacctc
    10441 acagaacgcg gaccaaaagc ctctcgcatc gaaaagcgtt tattgcaccc tcagcagcgc
    10501 aaggattgac ggcctgtttt tgatcgggat tctatcggcc tatcaccgtg gcacgcactg
    10561 caaaaattga ccgcaagacc agcgaaaccc agattgcgct ttccctgaat ctggacggca
    10621 acgcccgcat gtcaggcaat aacccgattc ccttctttga acacatgctg ggccacatcg
    10681 taaaatacag catgtttgac cttgagctcg acctcaaggg cgatattggc atcgattgcc
    10741 accattccgt agaagatacg gctatcgtct ttggccaggc gctcgtgcag gcgctcggca
    10801 acaagaccgg catcttccgt tacggttcgt tcacgctgcc catggatgaa gttttaacca
    10861 cggttaccat cgatctttcc gggcgcccct acttcgtcta ccgcggcgaa tccctaaagc
    10921 cgatgggtaa atttggcatc tatgattcgg agcttacgct cgagttcctg cacaagctct
    10981 ctatatacgg agcgatgaac ctgcatgtgc aggttcacta cggcgataac cggcaccata
    11041 ttcacgagtc tattttcaag gcgctgggct tcgcgctgcg ccaggcggta gcgatcgacg
    11101 cgcggcgggc gaatgaagtt ccttcaacga aagggagtct cacagggtag cctgaggctg
    11161 cgtcgtaggc taccctatga gcaagaaagc acgcataatc ctgttggatt tcggcatggg
    11221 gaatatacgc tctctgcaga aggcgtttga gcacctgggg caaagcgtca ccgtgtcttc
    11281 ggacccgaaa gaggcagagg ctgctgatgt gctcctgctg ccgggtgatg gtgcttttgc
    11341 ccgcgcgatg gaagagctca atacgcgtaa cctcgttgaa cccatctaca acgcgcgcga
    11401 gaagaagaag attatctttg gtatctgcat cggcttccag ttgctgttcg aaagctcaag
    11461 agagtttgct cttgcaaacg gcctcggttt cctgccaggg cacattaccc ggttagaaaa
    11521 aaatacacag gcgccggtga ttccccatat gggctggacc acgacagagt tttcgcccga
    11581 aagccggctc ggtaaaggca tagctccgca cagcatgttt tattacgtgc atagctatgc
    11641 atttgccggt cgtcacaaat atgcgaccgc cacgaccagc tacggtcagg tattcacctc
    11701 ggtgatcgaa cacgaaaacc tgttcgctgc ccagttccac ccggaaaaat ctcatacgcc
    11761 gggccttgcg ctgctgcgta actttatcga gcttctatga aactgattcc cgcgctggat
    11821 attctcgacg gcaaagtcgt acgtctcaaa cagggtgatt acaacgccgt taccatctac
    11881 cacgaaaagc ccaaagatct tgcatggtac ctggctgacc acggtgccga acgactacac
    11941 ctggtagacc tgcgcggttc aaaagagggc aaaatctgcg aaggcaggct cttcaccgaa
    12001 atacggcgcg cggtgaatat tccgtgcgag gcaggcggcg gcattcgcag cgcaacggat
    12061 tttcgttatt actacgacaa cggttttgaa gctggcaaag actttgtcat ggtgggttcg
    12121 ctcccctttg tcgatcctcc ggcgtttgat gccattctcg cagaatttgc ggggtcgctg
    12181 ctcatgacgg tcgacgcctg gggcacacag gtaaaacact cgggctggct caaggactct
    12241 ggtgtcgagc tcgatgattt tattgccggc ctcatcaaag aaggcgtgca gaattttttg
    12301 gtaacgcaga ttaaaaagga cggcatgatg gccggccctg actacgaaat gtacgaacgc
    12361 ctgctggcga aatttccgca gtcacgtatt atcgcatcgg gcggtgtcag ctcgatggat
    12421 gacctgaaaa aacttgcaac aatgaaactt tatggcgcca ttgtgggcaa ggcctactac
    12481 gaaggcaaaa taactgccga aatgctgagg aacttcaggc tttcgcgagg ctgaagctga
    12541 actcgcgcat ctttcgctcg acttcgcgat cgtagcgctt gtcaaaaatg ctttcggcgt
    12601 aaggcttgtg cgtgaatatc agcatctcgg cgccgtgcca ttcctgtacc tcgtttttcc
    12661 ggttgcgaat gcggccttcg ctcagcgcgc gcgagatttc gcgtgcgcca tggggcctga
    12721 ggtcgcgcat caatgcgcgg aaaaatgcct cgcgcgcggg atcataaaac cggaaggccc
    12781 ggtgaaataa taacgcgtcg tggaaatatt cgggtatgtt gaaaccgccg aacgcgtggg
    12841 tagcgaccac gagattgcgg agaaatatat cgatacggct aaagatgccg agcccgggca
    12901 catccttccc cgggaaaagt tcgcgcgtgt gcgtggcacc ccgcagtaca tggcgcgact
    12961 gcatccagtc aataaacagc attttcatgg ccggcatgag tggatgccgg tgcaggcgaa
    13021 aaaaagacag gcgcagccgc atgtgcagaa ttttctcatt gcggaacagc aggtagaacc
    13081 tgtggtcgcg ttcggtcaga aattctgtct ccagggatat cggccagtaa ccacgctcgc
    13141 gcaccgccgc gatgatatgg ctgtcttcga gcgcctgcca caactcttcg cggcttagcg
    13201 tacctagaaa aagtttttct cgcccgctaa agtgcgttgc cgcgggcagc tcgtccgcga
    13261 agtcgatctc ttcgagatcc tggaagtcaa agcgtttttc agacatagcg caaactggca
    13321 atacgaccga atcagtttaa ccttccgtgc agaatttcaa tcgtctgcgc atgcagtttc
    13381 accgtatgcg ccaggttgcg ggcataaccg cccgccggca gcacgaccag cggtacttcg
    13441 ggcatgaaat cgcgtacacg gcggtcgcgc tcggcaatac cctcgaaaga caactgcaat
    13501 ccaccgagca tgtcgtcctt gtagacatca accccggcaa tgtagaatat caggtcgggc
    13561 gcaaattgct gtcgcagttt agccaggttc tcatcgagta ttgccaggta ttcctcgtcc
    13621 ccgagaaatg aagcgaggtg aacatcgtgg ctgctctctt cttttttcgg gtagttttcc
    13681 ttctcgtgca tcgaaaaggt atagcagtct tcgtctttgc gcaaaatatg cgctgtacca
    13741 ttgccctggt gtacatcgag atcgataatc gcaaccttca aaccggggcg tgtttgcctc
    13801 aggtacttta tcgccacggc cgtatcgttg agaaagcaga agccttcggc atgatcggcg
    13861 aatgcgtggt gaaagccgcc gctcaggtta gagacagtgc cgtgtttcaa cgccagctct
    13921 gcggcgagaa ccgtgccctg cgtggctgta cacaccgcat ccacaatccg ctcgtcaatc
    13981 ggcacctctg cccgcagggt cttatcggtc agttttgcgc caaggaaatc acgcaggtaa
    14041 cgttcggtgt gcacaagccg cagggtcgct tcatcggccc gctgcggtgt gtgccagtgc
    14101 cagcgcgaat gcttgagctc gtcgtagagc atccggtact ttccggccga aagtacatgc
    14161 gcataatcag tcaggtcgta gaccggagaa tagaccaatt cgaggctttt ctttctcact
    14221 ttttattacg gcagaattcg cggaattttt cgctgccaaa tgaagcatcg acactggtca
    14281 tctcgggcat tttgtatggg tggttcttca tcatgtattc ttcgatacga tcatagtttt
    14341 cgcgcttcgc tttaataatg atttttgttt catcgtccag gttgatcttg ttctcccatt
    14401 tatagagaag cgtggtcccg ggaaaaagtg tgccgctgat gatcagcccc tcttcaagca
    14461 ggctggtcac caggtcctgc gccatttcgc tgtcattgat cgcggtaaag atcagtatct
    14521 cattggatgt cgacatggtg caccgtaggg gtgcccgcaa tagcgtaaag tgaagttgtg
    14581 ggattactct cccggggggt catttttact tgtagagggg tttttaccgc catttctgac
    14641 cgggtgtcta gtgcgccctc gctgcgtatc tccgtgcctg ccagtaccgc caacctcggt
    14701 cccggcttcg atatttgggg tatcgcgctc gacctgcgca atgacttcat ctgcgaccgg
    14761 gccgaccgca aagatggctc catcaaactc agcttcctga gcggcgacca gaccgtggtt
    14821 gcccccgcca gcctgccccg caaggttgaa aatgacaacc tgtttgtcag ggcttacaac
    14881 tacctgctca agaaggccgg ccagaccccc gtcgcgtggg atgtcaaaat aatcgtcaat
    14941 gtcccgtttt cgcgcggcct gggttcttcg agcactgcta tcctggcagg tctgaccctt
    15001 gccaatgaaa cgctccgccg cgattaccag atggcctacc gcatcgacca gattctcgac
    15061 ttcgcgctcg aatttgagcc acaccccgac aacctgactg ctgcgctcta cggcggctgg
    15121 aacctgtgca tgccactcgg gggccagacc cccaacatcg atgccgaaaa gggcgcctca
    15181 gggcctgcct tccaccggct gcccatcacg gtcaaagccc ctgtacgcat cgccggaatc
    15241 atacccgatc tc
//
LOCUS       DAOQJF010000028        14897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1914_length_14897_cov_5.027557, whole genome shotgun sequence.
ACCESSION   DAOQJF010000028 DAOQJF010000000
VERSION     DAOQJF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14897
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1914_length_14897_cov_5.027557"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="PLF85_02725"
     CDS             <1..2127
                     /locus_tag="PLF85_02725"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE18600.1"
                     /translation="FSIPDNVFYLRPYAEQADSAQPSQLRYIQALLPVYAGVFFFWFT
                     ALFLYVFSRVRRLERAYILFAAAVVTYLLLFVDFFTWHVSGNIFLFYNVLIIGPFLYL
                     FRSAYQLPTQGVLYLVILLLALGLYSFYPVRSAADELSFIKLLGGVFFATFVYCGYLF
                     FWSEGHREQIPGGRRLWAARIFSFSLIFAVALPPAFFFMLYYMPVHVDVNYNALFFLP
                     AFFPIIFITLSLRWGLVNFHIPISLAAVRFMYFAFFGFLYWFTVGFSLGELYQSEATQ
                     LRHFAILAAYLLLMDPARTALMLSLDSYATSRRKVLDGILLQSSGQIANPGRANSFIE
                     RLAVSLSEGLGCYWAKVIMSRDLFTNWDSDSEKIVYLPADDPFWVQAKTWVRGRKYPL
                     LTQTFIGPVRDFLQARGAFLVIGMDKFKAGIMVSERKDNLPFYSEDVRFLRQVAREAE
                     ILIQNYLFLIENVKLKRRERELAYNARIQKKVIPSSRQFEHFTFWSYSRAYESVTGDY
                     IDLMQTGPDRFVVLLGDVSGHGISSGYLVAFARAYLRGALTGRGESLRQAIGGLNTYL
                     AENYRGNEFITLFALQFEFKGDQVEIRYINAGQHPALIHTGKGLLRLEESQRLLGVIA
                     NEYHESELVIGRYPLRILLFSDGAFDVFNKSGKILGQKKFFDWVESSLEKSPADQLAF
                     LRQRIETYTASGEESDDLALIILEMK"
     gene            complement(2128..3426)
                     /locus_tag="PLF85_02730"
     CDS             complement(2128..3426)
                     /locus_tag="PLF85_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNE18601.1"
                     /translation="MSDTEATLREKIIKFGTWLFLMFIFIIIVVSFGMPDVIGTTSRL
                     DAYNAAKVGGEYLTKAEVADYQKRIEERMSANLKGLDDKNRKLFEDMAKSRALDEAID
                     RKIFTQLLNRAGYVPSSSTEPKILATFYKRQFGEYIVNGKLDMERLNEFLTPRRLTLE
                     QVGRSMLQEYGPAKAYEMLQGTAYASDYAILDEARFVTTQNSYRIVALDGAAKDKILR
                     GKFAPTEKEIQDKFKAEFQSKDPKAILDQSKRDSIKASLFNERRASLEKEFAQSLNQA
                     GKSGIAQVAALSGARLIAVDDIGLNADLDSKKGKDAAAISLAPLGQSEVFVRQRLSAP
                     VGTTIGPVEAGGYTYFFTVSARKNTPLPAAPAYGKLEGTAAVELGKLKNLPKEVAADK
                     LAESAGKNNYGQVLTAALEMHRNSIRVVRYNRSGTETAEK"
     gene            3522..4781
                     /locus_tag="PLF85_02735"
     CDS             3522..4781
                     /locus_tag="PLF85_02735"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNE18602.1"
                     /translation="MNEYITRIADNALEASQDLRQLSTGRKNAVLAELARLIQDNRKY
                     LQTENARDIAFARETALEASLVDRLTLSEKVIDGMSRAALEIAALPDPVGEVIEGRTL
                     ANGVRLSKVRAPLGVVALIYESRPNVTVDVGALCLKSGNAAILRGGKEAIHSNRALLS
                     LFHQALKSQGIPVEAVQLIEKTERALIIDLVKCTRQVDLVVPRGGEALIRFVTEHSLV
                     PVVKHDKGVCSIYVAADAAFDMARDIVLNAKTQRPGVCNAAECLLVHKDWPHTAALLK
                     ALSDSSVVMHSDSRGIEWGAQHGARVEPFLNDLGFGHEYLSLDISVRPVSGLDEAVRH
                     ILTWGSKHSEAIITNDYALAQSFVDALDSAAVFVNCSTRFHDGGEFGLGAEVGIATGK
                     LHVRGPMGLRDLTTTRYIALGHGEIRR"
     gene            4778..5347
                     /locus_tag="PLF85_02740"
     CDS             4778..5347
                     /locus_tag="PLF85_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNE18603.1"
                     /translation="MTFTGNPVFFFGGTFDPPHAGHKAIVIRLLKEHAGARVIVSPAP
                     AAPLRTDDRLFSYRERFALLRACFRQQLAEGKVILSRLEFSLPKPNFTFDTLAALVQV
                     CGTKPVIVIGADQAAQIGKWHRAEELMREYEFVVFAREGTTTAFDRRLRHHAISDFDL
                     PVSATVIRASLVKLPKAERFAAALALAGS"
     gene            complement(5379..6326)
                     /locus_tag="PLF85_02745"
     CDS             complement(5379..6326)
                     /locus_tag="PLF85_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18604.1"
                     /translation="MAGALHTPVSHEPGHTIFYLAGEDTAADRHNAALLARLARRSTT
                     PLYIEIFYHRPLQLSNWALATGDLRYTRLRSTSEFVLAERIAAARTETVLFFNFMIFA
                     HGEDLWRLLGHAAREPFALVLPAGTLYDRGETAWTFALRSARADLARYTGSPEQPAII
                     AMNRSLLRQNAARPGAGLLLRKFYADLLSGQQSPVPLLLKGRSRMFASTAAAWLPTLR
                     NTVFAYRAWRETRQFPWWFSYRHPLFLVAQLGVYTSLLVLPASLEGSVILLLFSVATT
                     LPYFLRGLWREIGICFSNVRRPAASLRRLAARLVLYLIG"
     gene            complement(6339..7226)
                     /locus_tag="PLF85_02750"
     CDS             complement(6339..7226)
                     /locus_tag="PLF85_02750"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE18605.1"
                     /translation="MVKYLKYLLPFALAVVAGCTYREGMLKSTIVSWAQVSPYGITMA
                     LAFYSAFLLIEREFPRVGLKVQLGDYILLAGVVGGIVGAKIAYVIEIRNTPEFQQGSL
                     FSHLFSGAGLTWYGGFILASAAIIFILWKNKVNILFGIDTLAPMLALGYVFGRAGCIF
                     SGDGCYGGLCTMNWPAPFCMAFPNGAASWSEWLARYANGNAAATAINTPFFESAFSFL
                     CFLFLWGVRKKQWPLGSKFFLYLFLHALFRFSIEFIRLNPRDVFGVSQAQFLSLLLMA
                     ISAGYVIVAFTRRSLVAPR"
     gene            7319..8056
                     /locus_tag="PLF85_02755"
     CDS             7319..8056
                     /locus_tag="PLF85_02755"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNE18606.1"
                     /translation="MFSRMVSGFTKPLESFSYLSQKGLWGYAVIPLALTTAIVVLFGV
                     GLVVYLAGVEGSLPALNTQDWPAVLRWLVSILGIVLKIIILLLLFALLMRVFLLLFSV
                     IVIPFMSPLVEKILVAEGITTLKISGTEMVGFIFAAIVYNVKLLIVQLFAALLLMLSG
                     PLQPALNFFVSGYFIGRSYFDYVFELLDRPQQFAQMARGNRAEATGVGIFSNLFVFVP
                     VVGAILAPLLCVVAATRIYAARNSGNK"
     gene            8060..9598
                     /locus_tag="PLF85_02760"
     CDS             8060..9598
                     /locus_tag="PLF85_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18607.1"
                     /translation="MYRALIGLLLLTGVLQPLSARLHLLVDERLRKSSLEQVIYRFVP
                     LTSAPEVELTYVRNFMQGEAAQVYAAKMREADRAIYIGADRYPGDAQIGALMKNRAWY
                     DLLGKIYFLVINDAPDYVPLPRGLFSHDDLWRKFAELSGARHAGFLNRHVLDNRVAAL
                     KDWRPGRGSCVHQSGALLVPSARGPIRFHRDGFSLSYRQKGQADMAGDWVCLNTPLAD
                     TIFQVRVARTDVLLAVKSARFSAWNDAQPLAVTLQSDRQISAPLALEFVPEVNSAETE
                     FFFQGDRLSCAALRCGKKGARFLLPEMAGLSQQFDLAFRGRADAYFRGTIRLMAGEIE
                     LARASVRLLPHSWLTEMAYALSHPSEYRLFFLYGLGGLILVLATLVLLYRFFRSWLGR
                     LQARRAEVLPAQSSAVLEVAPGDSLRLTATDNPFGCELLGFGGIVRVDVLGDGVDISH
                     GQGQGGRFHWADFRYALPDGYVLDFRPAGSRARLFVYRLSEKGLSATSVKPGQTLQSQ
                     QPQM"
     gene            complement(9525..10580)
                     /locus_tag="PLF85_02765"
     CDS             complement(9525..10580)
                     /locus_tag="PLF85_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNE18608.1"
                     /translation="MRRLLLCAVLMGLTGTAVSATPLEEKMRETTTEIESLWAEDACE
                     TVVIKKKGFPLASVACAGSFDNARRAKFLDVLFRHHFVLEKETYRLKPRNGTYLYKTT
                     QGRRTVYFKLYVRDADDLWPRNPVRGKQVAIHVQNLRSASDLVRWRTLGIPLTFGVTY
                     GRSDTAELLEKLEEYNEEKWLAIPLEDDRVDIADGNLLTIGDALDQEKIAEYLAALEE
                     TDGIDGISPLYCSRFCKNVPALRALLGAVRSSNGERALTLVDTDGSPVSSFYETGRIM
                     DFRTFRAYVTRPEKGNFCTVLRQFLEAPEANASRVIAVDAADEKALRCLREVTSAAAA
                     TVEFVRVSRMSLTNPFR"
     gene            complement(10585..11274)
                     /locus_tag="PLF85_02770"
     CDS             complement(10585..11274)
                     /locus_tag="PLF85_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18609.1"
                     /translation="MRPLRILPALLCLTAGLSAVSYEWGARSGESGFRLDVPEGWRTW
                     ESAKRNGVIVQFRRGSSRIEVRSMAAKDSFSTQQILNQKAARLSAEYPLVRYIGERPS
                     RYSAELTLTSWEIRVKGKTFIEESAVYLAPEGPVVVSCMVRQDELEKYRTHCENAFYS
                     LALGGAQPEKQVAKSDLTQNLQRLYLLHLPGNLQPVPPETLLTAPPPQTKPKPTIQYD
                     ENYILPAEENR"
     gene            complement(11345..11638)
                     /locus_tag="PLF85_02775"
     CDS             complement(11345..11638)
                     /locus_tag="PLF85_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18610.1"
                     /translation="MGRLLIILLALAALSSCSKPHLSVLPCDSDLGYLGEKAHFANVR
                     FLQESRQFDWFELYDNIEDRRSLKPSSNCIPMTATITTERAIERQEYSLRNLR"
     gene            complement(11683..12345)
                     /locus_tag="PLF85_02780"
     CDS             complement(11683..12345)
                     /locus_tag="PLF85_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE18611.1"
                     /translation="MLRNTILVATLLAPAFAFAGEAGKITFLSGQVDVDSGKGWQKAN
                     LGATLNESDKIRTGSRGTALINLKSGAVLKMKSDSQIILSQVGNATTVDVEAGALFSK
                     VGKRGNGQSFRIRAQTMVAAVRGTEFYFAYGKKKKSKADLWLCVNEGQVNVTDSATKS
                     EVDVNAGEGIVIPTDKQIPKPKAYNWTKKLNWNMDASQGEVEDKTDLKGAYKDLLRNN
                     YD"
     gene            12482..12901
                     /locus_tag="PLF85_02785"
     CDS             12482..12901
                     /locus_tag="PLF85_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system-associated lipoprotein"
                     /protein_id="HNE18612.1"
                     /translation="MRRTPLVLLSFLALGLVACGSRFVKKEEIQRISKDYEGVYVLRE
                     RVETGNFDSLNKGARVKIFFKAAGDYVSIYAYPYSQPREEAVGKNILQLFEGDFPDKK
                     FSEALLRQRIADLVEEYKGKPDAPAKPARAAKPAGRK"
     gene            12905..14146
                     /locus_tag="PLF85_02790"
     CDS             12905..14146
                     /locus_tag="PLF85_02790"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNE18613.1"
                     /translation="MKIGIFGLGTVGQGLLQILRDKKSEITIGAVVDRSFARKKEILL
                     DIPASADKNLILKDSSIDTVVELMGGIDDALYVVRTALENGKNVVTANKALLAEHGYA
                     LFEYAQRHNRFICFEAAIAGAIPIVRNIETIFRNEQITRLMGIVNGTTNYILTRMRKD
                     NMGYGDVLEAAQKAGLAEADPTLDVGGYDAQQKLALLISLITGSWVEAKNIPVRGITE
                     ITSQDVVWAERFHNRIRLVADYSVQEGRHYMHVTPALVSEQNVLYDIEYENNAILFHG
                     EYSGEHLFMGKGAGKFPTAFSVYSDLVHLLSGRAAWNHTQGEAYLPIANIADTSSAFY
                     LRLRVKDQPGVLAAIAQILGNNALSIASVHQDHSAVAAGEVDIVIHTHSARRSSFYKA
                     LKEIQSLKSIVLHQVYLPVLG"
     gene            14200..>14897
                     /locus_tag="PLF85_02795"
     CDS             14200..>14897
                     /locus_tag="PLF85_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNE18614.1"
                     /translation="MHDPMEILQFLAEHPVKSIFIALGVFLVLVAIRDITQKKRTIQH
                     NFPVVGRLRYMLEVIGPELRQYWVANDKEEMPFNRAERRWVYATSKGQNSNFGFGTTE
                     QLYEAGYPIIKHAVFPFPESKAVIIHDDPSAIPALKVIGESHRRAKAWRPPSIINISA
                     MSFGSLGDRAVRALNQGAKLAHCWHNTGEGGFSPYHTSGADVVWQIGTGYFGARDENG
                     FRLEKVVEKVDQFP"
BASE COUNT         3079 a         4068 c         4216 g         3534 t
ORIGIN      
        1 tttagtattc ctgataatgt cttttacctg cgtccttacg cggagcaggc tgactcggcg
       61 caaccttcgc aattgcgcta tatccaggct cttttgccgg tctatgcagg tgtctttttc
      121 ttttggttta ccgccctgtt cctctacgtc ttttcgcgtg tccgcaggtt agagcgtgct
      181 tacatacttt tcgccgccgc ggtcgttacc tacctcttgc tcttcgtgga tttttttacc
      241 tggcacgtgt cgggcaatat ttttcttttc tacaacgtac tgattattgg tcccttcctt
      301 tacctgtttc gcagcgctta ccagctgccg acgcagggtg tactctacct ggtgattctg
      361 ctcttagcac tcggactgta ctctttttat cctgtgcgat ctgcagccga tgaactgtcc
      421 tttatcaagc tactgggcgg agtgtttttc gcgacgttcg tctactgtgg gtacctgttt
      481 ttctggtccg aaggccacag agaacagatt cccggcgggc gcagactgtg ggcagcacgt
      541 attttcagct tctcgctgat atttgccgtc gcgctgccac ccgctttttt cttcatgctc
      601 tactacatgc ccgtgcatgt cgacgtgaac tacaacgcgt tgtttttcct gccggcgttt
      661 tttcctatca tctttattac tttgagcctg cgctgggggt tggtgaactt ccatattccg
      721 atcagccttg ccgctgtgcg tttcatgtac tttgcttttt ttggctttct ctactggttt
      781 actgttggtt tcagcctcgg cgagctctac cagagtgaag ccacgcagct ccggcatttt
      841 gcgattctgg ccgcatacct gctcctgatg gatcccgcgc gcacggcgct catgctctcg
      901 ctcgattctt atgccacttc ccgccgcaag gtgctcgatg gtatactgct gcagagttca
      961 gggcagattg ccaatcccgg tcgcgccaac agctttatcg aacgcctcgc tgtttcactg
     1021 agcgaaggtc tgggctgcta ctgggcgaag gtgattatga gccgcgacct tttcaccaac
     1081 tgggacagcg acagcgaaaa gattgtctac ctgccggctg acgatccctt ctgggtacag
     1141 gcgaaaacct gggtgcgcgg gcgaaagtat cctctgctca cgcagacctt tattggtcca
     1201 gtgcgcgatt tcctgcaggc tcgcggggct tttcttgtga tcggcatgga taagttcaag
     1261 gcgggtatta tggtttccga acgaaaggat aacctgccgt tctacagcga ggatgtgcgc
     1321 tttttgcgcc aggttgcgcg tgaagccgaa atactgattc agaactacct gttcctgatc
     1381 gaaaacgtca aactcaagcg ccgcgagcgg gagctcgctt acaatgcgcg tatccagaag
     1441 aaagttattc cttcttcgcg tcagttcgag catttcactt tctggtctta cagccgcgcc
     1501 tacgagagtg ttaccggcga ctacattgat ctcatgcaga cggggcctga ccggtttgtt
     1561 gtactgctcg gtgacgtgag cggccatggt atatcgagtg gctacctcgt cgccttcgcc
     1621 agggcatacc tgcgcggggc gctgaccggg cgcggcgaaa gcctgcgtca ggctatcggt
     1681 ggattaaata cttacctggc cgaaaattac cgtggtaacg aattcattac cttattcgcg
     1741 ctgcagttcg agttcaaggg cgatcaggta gagatacgct atattaatgc gggccagcac
     1801 ccggcgctca tccatacggg aaaggggcta ttacggcttg aagaaagcca gcgcctgctg
     1861 ggagtaatcg caaatgaata ccacgagtct gaactggtga tcggacgcta cccgctgcgt
     1921 atattgctgt tttcagacgg ggctttcgac gtctttaata aatcgggtaa gatactaggg
     1981 cagaagaaat ttttcgactg ggttgaatct tcgcttgaaa aaagcccggc cgaccagctg
     2041 gcgttcctgc gccagcgtat tgagacctat acggcttcag gtgaagagag cgatgacctc
     2101 gccctcatta ttctcgaaat gaaatagcta tttttctgcg gtctctgtac ccgaacggtt
     2161 atagcgcacc acgcgaatgc tgttgcggtg catttcgagc gcagctgtca gtacctggcc
     2221 gtagttattt ttccccgcag attctgccag cttatcagcc gccacttctt ttggcaggtt
     2281 tttcagtttg ccgagttcaa ctgccgccgt accctcgagc ttgccgtaag ctggcgcagc
     2341 gggcagcggg gtattcttac gcgccgagac ggtgaagaag taggtataac cgccggcttc
     2401 gacgggaccg attgtggttc ctacaggtgc cgaaagccgc tgacggacaa agacttctga
     2461 ctgcccgagc ggcgcaaggc tgatggcggc cgcgtctttg cccttcttcg aatcgaggtc
     2521 tgcgttgagg ccgatgtcat cgactgcaat cagccgtgcc ccggaaagag cagctacctg
     2581 cgcgataccc gatttacctg cctggttcag gctctgcgca aattctttct ccagcgaggc
     2641 ccggcgctcg ttgaagaggc tggccttgat gctgtcacgc tttgactggt cgagtattgc
     2701 cttcgggtct ttgctctgga attcggcctt aaacttgtcc tggatttctt tctcggtggg
     2761 cgcgaatttc ccgcgcagta ttttatcttt ggcggcaccg tcgagggcta caatacggta
     2821 cgaattctgc gtggttacaa agcgtgcttc atcgagtatt gcgtagtcag atgcataggc
     2881 agtcccctgg agcatttcat atgccttggc tggcccgtat tcctgcagca tagaacgtcc
     2941 gacctgctca agcgtcagcc ggcggggagt gaggaactcg ttcagccgct ccatgtcgag
     3001 cttaccgttg acgatgtatt cgccgaactg gcgcttatag aaagtcgcga gaattttcgg
     3061 ttcggttgag ctcgaaggca catatcctgc ccggttcagc aactgggtga agatcttgcg
     3121 gtcgatcgcc tcgtccagtg cgcggctctt ggccatgtct tcgaagagtt tgcggttctt
     3181 gtcgtcgaga cccttaaggt ttgccgacat acgctcttca atgcgctttt ggtagtcggc
     3241 cacttctgct ttcgtcaggt attcaccgcc caccttcgcg gcgttgtacg catcaaggcg
     3301 cgaagtcgtg ccgatcacgt caggcatgcc gaacgaaact acgataatga tgaaaataaa
     3361 catgaggaac agccaggtcc cgaatttgat gattttttcg cggagtgtgg cttctgtatc
     3421 agacattcgc caaggtggat ttttgattta ccccagcaaa ctgaaatcga cacggggggc
     3481 agaggggccc tgtgtaatac ttaccacgac cgccagctat aatgaacgaa tatattaccc
     3541 gaattgccga caacgcgctc gaagcctcgc aggatctccg ccagctgtcc accggccgca
     3601 aaaacgccgt actcgccgaa ctggcgcggc tcatccagga taaccgcaag tacctgcaga
     3661 ccgagaatgc ccgcgacatc gctttcgccc gcgaaaccgc gcttgaggcc agtctcgtcg
     3721 accggctgac gctttctgag aaggtcatcg acggcatgag ccgcgcggcg ctcgaaattg
     3781 cggcgctgcc agacccggtc ggcgaggtga ttgagggacg cacgctggcc aacggtgtca
     3841 ggctcagcaa ggttcgcgct cccctgggcg ttgtcgcctt gatctacgag tctcggccaa
     3901 acgttacagt agatgtgggg gcgctctgcc tcaaatcggg taatgcagcg atcttgcgcg
     3961 gtggcaaaga agcaatccac agtaatcgcg cgctgctatc gctgttccac caggcgctca
     4021 agtcacaggg tattcctgtc gaagcagtgc agctgattga aaaaacggaa cgcgccctga
     4081 ttattgatct ggtcaaatgc acgcggcagg tcgacctggt tgtgccgcgc ggtggtgaag
     4141 cgctgatccg ctttgtcacc gagcacagcc ttgtgccagt cgtgaagcac gacaaaggcg
     4201 tctgcagtat ctatgttgcg gccgatgcgg cttttgacat ggcgcgcgac attgttctga
     4261 atgcaaagac ccagaggccc ggtgtctgca atgctgcgga gtgtctcctg gtccacaagg
     4321 actggccgca tactgcggca ttgctcaaag cgctcagcga tagctccgtc gtgatgcaca
     4381 gtgacagccg tggtattgag tggggtgcgc agcacggtgc ccgggttgag ccgttcctca
     4441 acgacctggg ttttgggcac gaatacctga gcctcgatat ctcggtccgg ccggtttctg
     4501 gcctggacga ggctgtgcgg catattctta cctggggttc taaacactca gaagcgatta
     4561 tcacgaacga ctacgcgctg gcgcagagtt ttgtcgatgc actcgattct gccgcggtct
     4621 tcgtcaactg ctcgacgcgt ttccatgacg gtggtgagtt cggtctcggt gccgaagtgg
     4681 gcatcgcgac cggtaaactg catgtgcgcg gcccgatggg cctgcgcgac ctgacgacta
     4741 cccggtacat tgcgctcggg catggagaaa tccgccgctg accttcacgg gcaaccccgt
     4801 cttcttcttt ggtggtacat ttgacccgcc gcacgcgggg cataaggcaa ttgttattcg
     4861 cctcctgaaa gagcatgcgg gcgcgcgcgt catcgtttct cctgctcctg cagcaccctt
     4921 gcgcacagat gaccggctgt tcagctaccg cgaacggttt gcgctcttgc gcgcgtgttt
     4981 caggcagcag cttgccgagg gcaaggtcat actttcgcgc ctggagttct ctctgcctaa
     5041 gccgaatttc acctttgata cgctcgctgc gctcgtgcag gtctgtggca ccaaaccagt
     5101 aatcgtgatc ggggcagacc aggcagcgca aattggcaaa tggcaccgtg ccgaagagct
     5161 catgcgcgaa tatgaatttg tagtctttgc ccgcgagggg actacgaccg catttgaccg
     5221 acgcctgcgc catcatgcga tttccgattt tgatctgcct gtctccgcaa cggtaatcag
     5281 ggcaagcctg gttaaattgc cgaaagccga gcgatttgct gctgcgctgg cgctagcagg
     5341 ctcctgaaaa agaagccgaa agcaggttcc gaaatctgct agccgattag ataaaggact
     5401 agccgggccg ccagtcgtcg caatgaggct gccggtcggc gaacattact gaaacaaatg
     5461 cctatctcgc gccataagcc tctcaagaaa tagggcagag ttgtagctac agaaaaaagt
     5521 aggagaataa cactgccctc gaggctggct ggcaggacca gcagggacgt gtaaacaccg
     5581 agctgcgcca caaggaacag cggatggcgg taactgaacc accacgggaa ctgccgtgtc
     5641 tcacgccacg ccctgtacgc gaatacagta ttcctgagag ttggcagcca ggccgcggcg
     5701 gtcgatgcga acatgcggct gcggcctttg agcagcagcg ggacaggtga ctgctgtccg
     5761 ctgagcaggt cggcatagaa cttgcgcagc agaaggcctg cgcccgggcg cgctgcattt
     5821 tgtctcagca ggcttcggtt catggcgata atcgccggtt gttccgggct gccggtgtag
     5881 cgtgcgagat cagcgcgcgc actgcgcagc gcgaaggtcc aggcagtctc accccggtca
     5941 taaagcgtac cggcgggcaa aaccagcgcg aacggctcgc gcgccgcgtg gccgagcagg
     6001 cgccagaggt cttcgccatg ggcaaagatc atgaagttga agaacagtac ggtttccgtc
     6061 cgggcggcgg caatacgctc ggcgagcaca aattccgagg tggagcgcag acgcgtgtag
     6121 cgcaggtcac ccgtagcgag cgcccagttg ctcagttgca gtggccgatg gtaaaagatt
     6181 tctatgtaca gcggcgttgt cgagcgccgc gcaagacgcg cgagcaaagc ggcattatgc
     6241 ctgtcggctg cggtgtcttc gcccgcgagg taaaaaatcg tatggccggg ttcgtgagaa
     6301 actggcgtat gcagcgcccc ggccatttgg ttgcgcgctt agcgcggtgc tacgagcgag
     6361 cggcgcgtaa acgcgacgat cacgtaaccc gccgagattg ccatcagcag cagggacagg
     6421 aactgcgcct gtgagacgcc aaagacatcg cgtggattca gtcggataaa ttcaatcgag
     6481 aagcggaaca gtgcatgcag gaagaggtag aggaagaact tggagcccaa tggccactgt
     6541 ttcttacgca ctccccacaa gaaaaggaaa cagagaaaag agaatgccga ttcaaaaaaa
     6601 ggcgtattga tcgccgtcgc agctgcattg ccgttggcgt atcttgccag ccactcgctc
     6661 cagcttgcag caccgttggg gaaagccata cagaaaggag ccggccagtt catcgtgcac
     6721 aatccgccgt agcagccgtc gccggaaaaa atacagcccg cacgcccaaa aacgtaaccc
     6781 agcgccagca tgggtgccag ggtatcaata ccgaacagga tatttacctt gtttttccag
     6841 aggataaaga tgatggcggc gctcgcgaga ataaaaccgc cgtaccaggt gagccctgcg
     6901 cctgaaaaca ggtgcgagaa cagtgaacct tgctggaact cgggcgtatt gcggatttca
     6961 atgacgtacg cgatttttgc gccgacaata ccacctacaa cgcccgccag caggatatag
     7021 tcgccgagct ggactttgag accgacgcgc ggaaactcgc gctcgatcag gaggaacgcg
     7081 ctgtaaaagg ccagcgccat ggtaatgcca tagggagaaa cctgggccca cgagacaatc
     7141 gtgcttttga gcataccttc gcggtaagtg cagcctgcaa cgactgcgag ggcaaacggc
     7201 agcaggtatt tgagatattt aaccataagg cacagacggc gctaaaaacg cgcggtcaac
     7261 cgaaaggagc gcccaaaact tgctttacga caacgatctc ctgccgcaat ttacggttat
     7321 gttcagtcgc atggtcagcg gttttaccaa accgctcgag agtttttcgt acctcagcca
     7381 aaagggtctg tggggttacg ctgttattcc gctggcgctg accactgcaa tcgtggtact
     7441 attcggtgta gggctagtgg tctaccttgc gggcgttgaa ggcagcctgc ccgcgctcaa
     7501 tacgcaggac tggccggccg tactgcgctg gctcgtgagc attctcggta ttgtgctcaa
     7561 gatcattatt ctgttgctgc ttttcgcatt gctgatgcgt gttttccttt tgctgttttc
     7621 agtaattgtt atacccttca tgtcaccact ggtcgaaaaa atactcgtcg ccgagggaat
     7681 tacgactctg aagatcagcg gtacagaaat ggttggcttt attttcgcgg ccatcgtcta
     7741 caacgtcaaa ctgctcatcg tgcagttatt cgccgcgctg ctgctgatgc tctcagggcc
     7801 gcttcagccc gcgctgaatt tcttcgtatc cggctacttc atcgggcgtt cgtactttga
     7861 ttacgtgttt gaattattag acaggccgca gcagtttgcg cagatggccc gcggtaaccg
     7921 cgccgaagct accggcgtcg gtatcttttc gaacctgttc gtctttgtac ctgtagtcgg
     7981 ggcaatcctg gcgccactgc tctgtgtcgt cgcagccaca cgcatctacg cggccaggaa
     8041 ttccggcaat aagtaacccg tgtaccgcgc gctgataggc cttctgcttt tgaccggcgt
     8101 tttgcagccg ctgtctgccc gcctgcacct gctggtcgat gaacgattgc gcaagtcttc
     8161 gctcgaacag gtaatctacc gctttgtgcc gcttaccagt gcacccgagg tggagttaac
     8221 ctatgtccgt aattttatgc agggcgaagc tgcgcaggtc tacgcggcaa agatgcgcga
     8281 agcggatcgc gcgatctata tcggcgcaga tcggtatccc ggtgacgcgc agattggcgc
     8341 gctgatgaaa aacagggcct ggtacgattt gctgggtaag atttatttcc tcgttatcaa
     8401 cgatgcgccc gactatgtgc cgctgccgcg cggcctcttt agtcacgacg atctttggcg
     8461 caaatttgcc gaattgtcgg gtgcccggca cgctggtttt ttaaatcgcc acgtgctcga
     8521 taaccgtgtc gcggcactca aagactggcg tcccgggcgc ggcagctgcg tgcaccaaag
     8581 tggcgcgctg ctcgtgccct cggcccgggg gcctatccgt tttcaccgcg atgggttttc
     8641 tctctcgtac cggcaaaaag ggcaggccga catggccggt gactgggtat gccttaacac
     8701 tcccctggcc gatacgattt tccaggtgcg tgtcgcacgc accgatgtac tactggctgt
     8761 aaagtctgcg cgtttctctg cctggaacga cgcgcaaccg ctagcggtga cgctgcaatc
     8821 ggaccgccag atttcggcgc cgcttgcgct cgaatttgtg cccgaagtga attccgctga
     8881 aacggaattc ttcttccagg gcgaccggct gagctgcgct gcgttgcgct gcggcaagaa
     8941 gggcgcgcgc ttcctgctgc ccgagatggc cgggctcagc cagcagtttg atcttgcctt
     9001 tcgcggccgg gctgatgctt atttccgcgg aacgataagg ttaatggcag gtgaaatcga
     9061 acttgcccgg gcttctgttc gcctgctgcc ccacagctgg ctcacggaga tggcgtatgc
     9121 actttcacat ccttcagaat accggctttt ttttctctat ggtttaggcg gattgatact
     9181 tgtactggct acattggtcc tgctataccg gtttttccgt tcgtggttag gcaggttgca
     9241 ggcgcgcagg gctgaagttt tgcctgcaca gtcgagcgcg gtgctagaag ttgcaccggg
     9301 cgatagcctg cggctgacag caacagataa tccctttggc tgtgagttgc tggggtttgg
     9361 cggcatcgtg cgcgtcgacg ttctgggcga cggagtagac atcagccacg gccaggggca
     9421 ggggggccgg tttcactggg cagatttccg gtatgctttg cccgacggtt atgttctcga
     9481 ttttcggcca gcgggcagcc gtgcccggct gttcgtttac cggctcagcg aaaagggttt
     9541 gtcagcgaca tccgtgaaac ccggacaaac tctacagtcg cagcagccgc agatgtaact
     9601 tcacgcaggc agcgtaaggc tttctcgtct gccgcatcaa ctgcaataac gcgcgaagca
     9661 ttggcttcgg gcgcttcgag aaactggcgc agcacagtgc aaaaattgcc tttctcgggc
     9721 cgggtgacat atgcacgaaa agtacggaag tccatgatcc tgccggtctc atagaaagaa
     9781 gagaccgggc tgccgtcggt gtccacgaga gtcagtgcac gttcgccgtt gctgctccgc
     9841 accgcaccga gtagtgcgcg taacgccggt acgttcttgc agaagcgcga gcagtacagc
     9901 gggctgatac cgtcaatgcc gtctgtttct tcaagggctg caaggtactc ggcaattttt
     9961 tcctggtcga gcgcatcgcc gatcgtcagc aggttgccgt cggcaatatc gacgcggtca
    10021 tcttcgagtg gtatggccag ccatttttcc tcgttgtatt cttcgagttt ctccagtagc
    10081 tccgccgtat cgcttctgcc ataggtcacg ccaaaggtca gtggaatacc cagtgtgcgc
    10141 cagcgcacga gatcgctggc cgagcgcagg ttctgcacgt gtatagccac ttgcttgccc
    10201 ctaactgggt tgcgtggcca gagatcatct gcatcgcgca cgtagagctt gaagtacaca
    10261 gtcctgcgtc cttgtgttgt cttgtagagg taggtgccgt tacgcggctt gagcctgtac
    10321 gtttcttttt cgagaacaaa atggtgccga aacaggacat cgagaaattt tgcccgccgg
    10381 gcattgtcaa agctaccggc gcaggctacc gaagcgaggg gaaatccctt tttcttgatc
    10441 acgactgttt cgcaggcgtc ttctgcccag agtgactcga tttccgtagt cgtctcgcgc
    10501 atcttttcct ctagtggagt agcagagact gccgtgccag tgaggcccat gagtacagcg
    10561 caaagtagca gtcgacgcat agagttaccg gttttcctcg gcaggaagga tatagttttc
    10621 atcgtactgg attgtcggtt ttggttttgt ctgcggtggt ggcgcagtca atagggtttc
    10681 aggggggacc ggttgcagat tgccgggcag gtgcagcagg tacagtctct gcaggttttg
    10741 cgtgagatcc gattttgcca cctgcttttc cggctgggcg ccgcccagtg ccagcgagta
    10801 aaaagcattt tcgcagtgcg tgcggtattt ttccagttcg tcctggcgca ccatgcagga
    10861 aaccacgacg gggccttcag gggcgaggta cacagcgctt tcttcgatga atgttttgcc
    10921 ttttacgcga atctcccaag aggtcagcgt cagttctgcc gagtagcgtg aaggtctttc
    10981 gccgatgtat ctcacaaggg gatattctgc agacaggcgc gctgccttct ggttgagtat
    11041 ctgctgcgta ctgaatgaat ctttcgccgc catgctgcgc acctcgatac gtgagctgcc
    11101 ccgcctgaac tggacaatta caccattgcg tttggcagat tcccacgtgc gccagccttc
    11161 aggaacatcc agccggaaac ccgactcgcc tgagcgcgca ccccactcgt agctgactgc
    11221 actgagtcct gcggtgagac ataataaagc aggcaggatg cgtagcgggc gcaaagacat
    11281 cttctttata atcggtctgt tacggggacg aagtcaagca ccgccgccgg aatttactga
    11341 aatactacct gaggttacga aggctgtatt cctgacgttc tatcgcgcgt tcggtcgtga
    11401 tggtcgcagt catggggatg cagttgctcg agggctttag gctgcgacga tcttcaatgt
    11461 tatcgtagag ttcaaaccag tcaaactggc gtgattcctg caggaaccgg acattggcga
    11521 agtgcgcctt ttcacccagg tatcctaagt cagaatcgca gggcaggaca gacaggtggg
    11581 gcttgctgca tgagctcagg gctgccagcg cgagcagaat aataagcaat cgccccatca
    11641 cgttatctta tcggccctgc actttgcgaa ccttacctgc gatcagtcgt aattgttgcg
    11701 caacaggtct ttgtaggctc ccttcaggtc ggtcttgtct tctacttcac cctgcgatgc
    11761 gtccatgttc cagttcagct tctttgtcca gttatatgcc ttcggtttgg gtatctgctt
    11821 gtcggttggg attacaatac cttcaccggc attcacgtcg acttcgcttt ttgtcgctga
    11881 gtcagtgaca ttgacctggc cctcgttgac gcagagccag aggtcggctt tactcttctt
    11941 ctttttgccg taggcaaaat agaattctgt accgcggacc gcggcgacca tggtctgggc
    12001 cctgatacgg aaagactggc cattgccgcg tttgcctacc ttggaaaaca gcgctcccgc
    12061 ttcgacgtca accgttgtgg cattgcccac ctgcgacagt ataatctgcg aatcggattt
    12121 catcttgagc accgcccctg atttcaggtt gatgagcgct gtaccgcggc tgccggtacg
    12181 gatcttatcc gattcattga gtgtggcgcc gaggtttgct ttttgccagc cctttcctga
    12241 gtcgacatcg acctgtccgg agaggaaagt gattttgccc gcttcaccgg cgaacgcaaa
    12301 agcaggtgcc agcaaggttg ccaccagaat agtatttctc agcattagaa ctccttgggg
    12361 ccgcagcccg gtataagagc atcgtggtga tgcagctgag caggcaagta aaaattcgaa
    12421 ctgccggtgc ctggctcata tcttacttta cgcggtgaaa cttttaccgg tttcacgatt
    12481 aatgcgccgt actccccttg ttctcttaag ctttcttgcg cttgggctgg ttgcctgtgg
    12541 cagccggttt gtaaagaaag aggaaatcca acggatcagc aaagattacg agggtgtcta
    12601 tgtgctgcgt gaacgggtcg aaacgggcaa ttttgacagc ctcaataagg gcgcccgcgt
    12661 gaaaatattc ttcaaggccg cgggagacta cgtgtctata tacgcatatc cgtattcaca
    12721 gccccgtgaa gaagcagtcg gcaagaacat tctgcaactc ttcgaaggcg attttcccga
    12781 taaaaaattc agcgaagcgc tgttgcgcca gcgcatagca gacctggtcg aagaatacaa
    12841 gggcaagccc gatgcgccgg caaaaccggc cagggcagcg aagccagcgg gcaggaaata
    12901 gcatatgaaa atcggcatct ttgggctggg tacggtcggt cagggtttac tgcagatact
    12961 ccgcgacaaa aaaagtgaaa tcacgattgg tgcggttgtc gaccgcagtt ttgcccgcaa
    13021 gaaagaaatc ctgctggata ttccggcatc agcagacaag aacctcatcc tgaaagacag
    13081 ctcgatcgat acagtggttg aactcatggg cggcatcgat gatgcgctct acgttgtccg
    13141 caccgcgctc gagaacggca agaatgtcgt gacagcaaac aaggcgcttc tcgccgaaca
    13201 tggctacgcg ctctttgagt atgcgcagcg ccataaccgt ttcatctgct ttgaggccgc
    13261 gattgccggt gcaattccga ttgttcgcaa tatcgaaacc attttccgga atgaacagat
    13321 cacacggctc atgggtattg ttaacggcac gacaaactat atcctgacgc gcatgcgcaa
    13381 ggacaacatg ggttacggag atgtgctcga agcggcacaa aaggcagggc ttgccgaggc
    13441 tgatccgacc ctcgacgtcg gtggttacga cgcgcagcag aaacttgccc tgctcatttc
    13501 gctgattacg ggcagctggg tcgaagcgaa gaacattccc gtacgcggca ttaccgaaat
    13561 tacttcgcag gacgtcgttt gggcagaacg cttccataac cgtatacgcc tcgtcgccga
    13621 ctattctgtg caggagggcc gccactatat gcatgtgact cccgctctcg tcagcgaaca
    13681 gaatgtcctg tacgatatcg agtacgagaa caatgcaatt ctttttcacg gtgaatattc
    13741 cggtgagcac ctgttcatgg gtaagggagc gggtaagttt cccacggcat tttccgttta
    13801 ttcagacctg gtacacctgc tttccggccg cgccgcatgg aaccacacac agggcgaggc
    13861 atacctgccg attgcgaaca tcgctgatac cagctcggcc ttttaccttc gcctgcgcgt
    13921 gaaagaccag ccgggtgtgc tcgcagccat cgcgcagatt ctcggcaaca atgcactctc
    13981 catcgcgtcg gtgcaccagg accactcggc ggttgccgcg ggtgaagtag atattgttat
    14041 tcacacgcat tcggcgcggc gcagctcttt ctacaaggcg ctgaaagaaa tccagtcgct
    14101 gaagagtatc gtgctgcacc aggtttacct gccggttctg ggttaagcat tactaaagca
    14161 ctgggcgtaa aagggcttgt aaatctgcga agaagctgaa tccatgaccc catggaaatt
    14221 ttgcagttcc tcgctgaaca cccggtaaaa agcatcttta tagccctcgg ggtatttctt
    14281 gttcttgtcg cgatccgcga catcacgcaa aagaagcgca ctatacagca taactttcct
    14341 gtcgtcggcc gcctgcgtta tatgctcgag gtcatcgggc cggagcttcg gcagtactgg
    14401 gtggccaatg ataaagaaga gatgccgttc aaccgcgctg agcgccggtg ggtctatgcc
    14461 acctcgaagg ggcaaaacag taactttggc ttcggtacca ccgagcagct ctatgaagca
    14521 ggttacccga tcattaagca tgcggttttc ccgtttccgg aaagcaaggc agtcattatc
    14581 cacgatgatc cttcggctat accggcattg aaggtcatcg gtgaatctca ccgcagggcg
    14641 aaggcatggc ggccgccgtc tatcatcaat atctccgcga tgtcctttgg ttcgctgggc
    14701 gaccgggctg tacgtgcgct caaccagggg gcgaagcttg cgcactgctg gcacaataca
    14761 ggcgaggggg gattttcccc ttaccacacg agcggcgccg acgtggtatg gcagatcggt
    14821 accgggtatt ttggcgcgcg tgatgaaaat ggctttcgcc tcgagaaagt agtcgagaag
    14881 gtagaccagt ttccaca
//
LOCUS       DAOQJF010000029        14835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1927_length_14835_cov_5.164614, whole genome shotgun sequence.
ACCESSION   DAOQJF010000029 DAOQJF010000000
VERSION     DAOQJF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_14835_cov_5.164614"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PLF85_02800"
     CDS             complement(<1..1152)
                     /locus_tag="PLF85_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE18615.1"
                     /translation="METTADVFPYGVTRRDGAWLFAVLVTAAEQVTLCLFDREGQLLD
                     RIPLTAAGDRWQVSVVRDLAGELYCYEVTRQGETLYLADPFARSMNQLHIWRDSKTWP
                     APVAEISQPGFDWGDDRLPQIPVPQMVIYEAHVKGLTIEFPGMPDEMRGRYGGLHHEH
                     LVAHLKSLGVTSVELLPVHGKSEDPYLTSKGLTNYWGYNTLAYFAPESGYAAGDAVTE
                     FKTMVRELHRAGIEVILDVVYNHTGEGAHDAPAVSFRGLAEGVYYRRAEDGSYIDYTH
                     CGNTLNTDHAHTRDLVLQSLRYWAEEMHVDGFRFDLAPAIFRKNGAVDFHHELHAAIV
                     GDPVLRERKLIAEPWDLGPDGYQRGRFPQPWLEWNDSFRDTARRFWKGEF"
     gene            complement(1152..2741)
                     /locus_tag="PLF85_02805"
     CDS             complement(1152..2741)
                     /locus_tag="PLF85_02805"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNE18616.1"
                     /translation="MNIFEISSECSGLAQRGGLGGVVWGISDAFRQSGHKVTLVMPYY
                     EEIREEVKSYLTLKVPYGGEISTVSVFASEHEGIRILLIHSDRFFRGEYSDVYIDSGR
                     LQRGFFEDDAKRFAFFSVAAAWLLLRLSRKEKIDTIHCHDWHSGLLLMLINVHPDLAE
                     LRKYFRLFSVHNLEYQGTRPYAAWGDLKGFLDWFPELEQLPRETMAPYLDPYANIDCI
                     NPMRAAIHSADQVTTVSPNYADEICRADNAVEGFFGGRGLEKDFRELRIAGRLTGITN
                     GIDLSFYDPAVLPHAYSAASRAAGKLRNREALWQELPHLLAGFRESESLTEESIADTL
                     ARLGRTDEQHFMTKPLMVAVTRMTGQKIRQFFDEAEGKSVLRHLMETGANYVFLGKGD
                     MAPQMSHEIAPAGNMFFLCGFDDNLEKRLYAAADAMLMPSDFEPCGTSQMKSMRYGCV
                     PIAARVGGLANTIINEHNGFLYDGASRTEKASAFLQKIRHVIALHNEEPEALLKMQAN
                     AMVTDFSWNEAAASYLRLMRQ"
     gene            complement(2738..5131)
                     /locus_tag="PLF85_02810"
     CDS             complement(2738..5131)
                     /locus_tag="PLF85_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNE18617.1"
                     /translation="MQKVSLYTVLPLVASSVIVWAQAQEGKIRLDRPQPAAASETPAQ
                     PAATAQTKADTAEKKPGLAELEELKSGFKIPKPFFFNMYVRASAQDDFGYNAPTLGWS
                     PFYGRLMNETPWLMADFGYHVVRPETPGAPRATLFMRIEGGSYRGYNMGNGSLSNFSI
                     NHVWLETENVLFKTLVTQTGNLWYNMGYIGIFDNFTAQVFYDTVGFRAGQRTKLFEYF
                     LGAGDSGWSLYRNRSYDGWEGHQYAVIPTVGGLVKLNLYELDGLKSFSNKFMPRWQVG
                     ASFQLMWERANETMRGAPYQTPNVAYADVMRGEAFKKFMLENPGNSDFFPWAQPHPTN
                     YWRYTGWTGFSGPDLGPIKIYWNDLSVRFEKKAPQIAVRETYDNESKDIYIAAFTDER
                     YELSLYDEIQLRLLPNHFDLNAGFGFGKSWDNDNKLRPDDYNSTRYSFVIRPMWYITE
                     HLHYLVELVYAREKSNIGNRYREHFNSIKSNTQGIPDSEGLEWGDTDTKHTYQIKTGF
                     TINPAGRGIYSRPVIRLLYGVQHSNVQAAFGNSFEESLNRRNQFNLNRDIHWHHMVRV
                     EFEHWFSVSGWEEGYTPPLAQQTERLSQVHKGVEIDPTKPKRYDIYTTIWGGWMFQFG
                     PLTTGLADRLVSDPDGCLGLTSRQCVINYNSGGVSGGAHVRVRSGNIEYTGGLWYLPV
                     YSASIFRSTGNAESVVSFFMASAGFRYFPEFLPEALRGIFAGVEVGYGIPLIQNSVAG
                     SPKSLDLGTYITGALSFGYQYRLGSSVSIEAGTQVIVMPTKNVMLGAMPYVGASYHL"
     gene            complement(5148..7712)
                     /locus_tag="PLF85_02815"
     CDS             complement(5148..7712)
                     /locus_tag="PLF85_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE18618.1"
                     /translation="MCILFFGKCAGPNSDFFRMYDAEPHAPSFNTAQIKNMEVMGPKI
                     VDGGVNFTLFSERATRVHLLIFDNPDSKYPVQEFQMSRFGNVWSVFINGVGHGTYYGY
                     RVWGPNFDYHPSWYPGTLFGFKSDFDNQGNRFNPNKLLLDPYAKAVHRKHDWSKGMAA
                     SGPYRHEDTTAAAAKGIVVRDDNYSWSSTEQDYWQRKQSTTQRRARNSLVFYEVHPKG
                     ISAAALAGIPGLPADYHARFPGTYRGTGELAPYLADLGITAVEFLPIHEAGSDGGYWK
                     YWTLNFFTPEFTYAYRPEKASQVNEFRWMVEQFHKYNIEVIIDVVYNHTGEGGLWREK
                     VPLIEGNVPVNPNDPSNFWAYDPAETATILSYRGIDNYSYYALTGPNKEFYYDHTGTG
                     NMVRTGSPPVRRMVIDSLRYWVEQMHVDGFRFDLSEVLEKKDGQATNNWSSYNYWKEW
                     GSETYSSTNTSRRNMSASMWIVNDPVLQRYDTRIIAEPWSIGGYRFGDHPKAYCYGTD
                     GDKYAGYETGCTALDTTYTTGFAWYEWSDLFKRVSRRIINNDDYRLNSDSPVDFGGAL
                     TGSSAKFNETWDGRQPYHNINKITAHDGFTMYDLLSYNQKRNGLGPLNPDGVDPYSGD
                     DGSNYSRDWGFNENLKRQNFRNLLVLLMVANGSPMLLGGDEWMRTQFGNNNAYTTGSD
                     NQYNWYRWGVWLADPYAYRMYDFTRKIIRFRKDNLWAFSKNNYPNMGDLSWHDIGASP
                     GNCNASFAGSGAWGGSDKVIALCYKNPPSGKTEIFIVFNLGGVNTHTINLPTGTWNVV
                     TNTEYYFEGFHANLSGNYCANSGEATFNPSAPRCNASTASGTYSLPPRTILILQKQ"
     gene            complement(7751..>8943)
                     /locus_tag="PLF85_02820"
     CDS             complement(7751..>8943)
                     /locus_tag="PLF85_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE18619.1"
                     /translation="LDNIKYWVKHYHVDGFRFDLAVGTSEQTLRDIQNMINDAAQPGA
                     WTTTVPPSATQTSMLIDSEFLRSSATETVTVDGNTRDNFNNIIKLPNFMTGENWLGAQ
                     RHGFMVQSTWTTPNFASQLSTDYRGFSQWNDYFRESIKRFIQGNTDATVTNRSKAAIY
                     FSKGRDDGVWQGVSNNPLDTLNYIESHDEESVARAVLDSKSKSAIGSLLLLTAHGIPM
                     LYEGQEFMRNKQIQDQSKIGNNLDWQLAATNADMLGFVSFLTKLRRACPALRYASDPG
                     NSNSNVDGATPNQTISYFTKGGACSGFSGDSSTYINTKSEFRILANMTGGAQAFTVET
                     GGANWRAVAAVSGGNDNTNCGDQSAATLRWSYASGSNNINSWGTTNSSWSVPCYSAVI
                     LVKQ"
     assembly_gap    8944..9043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9044..10452)
                     /locus_tag="PLF85_02825"
     CDS             complement(<9044..10452)
                     /locus_tag="PLF85_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE18620.1"
                     /translation="MRRRAAAVISVLLMTAFAMRCNSEQKPRTYIPVIDLTDAQGNVI
                     DTPCSNPQAASNYKQPTATDWPTIRDMKLGAVYDGTNTTFRFFAPTAQQVEVVLYNAA
                     TDSMFSPSAVINMTRDGTGNAGFGVWSTAAITTAEAKPVSAAGAGKYYYRYRINCMQN
                     YENKTIAERDRYITDPYALASAGSHGHGIIVDTQWLANAVFSGWNNATDGVTSDIGGA
                     NWFGTARPPSSQYIVYEAHVDDFSGSTSCLQSHSGSTCKTQLQAYYSATTPNNVALMT
                     AVGATSGDNLSGKYLGFEAMVSATTTPTVTSSHLSSTMGVNAVHLMPLHESSNDNKDN
                     PESYSWGYLPSLLFAPESSYSSYCSRANASEACTYVDARAGYQVLELKRMIKKMHAQG
                     IAVILDVVLNHTSNAFNYLYQADPYGRYYFRTNGLNWSNVGTGNQIFDDRNQRPFAYK
                     LVLDNIKYWVKHYHVDGFR"
     gene            complement(10614..11852)
                     /locus_tag="PLF85_02830"
     CDS             complement(10614..11852)
                     /locus_tag="PLF85_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE18621.1"
                     /translation="MRRISIAAIILLGLACSQDTKTKPAEPRTLTLWHSYNNEETRVF
                     NDLLADYQKQNPHIKIVAERVPFDGLLPKLTSAAIAHRTPDIARVDIGHIPRLAWGKA
                     IAPLGEFGAEKLVSDMLPVARKVAEVKLPGRKSPEIFAVPDQLTTVALYYNTKLLAAA
                     GVTPPRTIGELSKMGELFRKQGNYAKAFAMNSSLWWMMPWLFLHNAQILSGDLDRCLL
                     DSTEAVSAVEYLRALYASGSEGGAWVSGAVNPDQGFLTGRYAMILSGPWNLQTFRTVP
                     FAVSLVPGNGSLHSASNIGGAAMVVFSASKEKDESYKLLAYLVSQEVQKKWISGTGQL
                     SVNRAANRAMAGKLSAELKVFLQQLDYAGARPQLPGYDALETIVAPYLYAALDGSLPA
                     KEALRRACAEIERDLLAPNR"
     gene            complement(11906..12742)
                     /locus_tag="PLF85_02835"
     CDS             complement(11906..12742)
                     /locus_tag="PLF85_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNE18622.1"
                     /translation="MRRLKLILLWISIVALAAFALLPLFWMFVTALKKEGMGLSTQII
                     PRTGFAELYTWKNFSAILGDPDFPFFRFLLNSTAVAGSTAVISTAICFMSAYAFARKT
                     FVGKELVYGLLMASMMVPGMIFMVPQFALIAEFGWMNTWQALVIPHTANVFGIFLLRQ
                     SIERIPRSLFEAAEVDGASDWQTMRLVILPLASPVLVTLVLLTFLGQWSNFLWQLIVN
                     TPDSSWITLPVGISLFKGQYSIAWEKMMAASVISVLPVLLLFLFLQRYIISGLTQGGV
                     KE"
     gene            complement(12739..13641)
                     /locus_tag="PLF85_02840"
     CDS             complement(12739..13641)
                     /locus_tag="PLF85_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE18623.1"
                     /translation="MRRFAPYLWLTPFLAVFAVFLAYPMAYSFIISLKKVTWQTNLYE
                     VFSDMRWVGLQNYRELLTRADFWWSLVATFLYMLLTIPPGIFLSLLLATLLSENLPGS
                     RFFRSALFLPNILDMLVIGLVWKLIYSPDGLLDTAFMKMGVEYFHAKGFLGDAHTALA
                     AVAIAMVFKGCGFGMVLFMTALKNISPNIFEAADLDGLTWWQKFRYIRVPLVKPVILF
                     LTVTGILGCLNAFTEFYIMTEGNPVTLFANETVGATRVSGYLLYTRFTEMNYGSAAAM
                     SYFLLIFALIVSYLNWRFLRERTA"
     gene            complement(13683..14042)
                     /locus_tag="PLF85_02845"
     CDS             complement(13683..14042)
                     /locus_tag="PLF85_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE18624.1"
                     /translation="MKFPVDTNIAIYQLQNRLAERLPEGRYFLSIISEIELLAYPGLT
                     TAEEKGIKTLLKAIEIVTLTDSVKQETIRIKRDNNLKLPDAIIIATARINGCVLLTND
                     QQLAKSRLIDTKAFRLR"
     gene            complement(14039..14332)
                     /locus_tag="PLF85_02850"
     CDS             complement(14039..14332)
                     /locus_tag="PLF85_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18625.1"
                     /translation="MKKNLKNTGVRRSRPAASPMKAYSIVKDRSSKPLFVLVPYEVWQ
                     ETKKANRAKSAKQKPKHRRKKTEKVPPWADLAGVLGEREDALEYQKRLRGEWS"
     gene            complement(14349..>14835)
                     /locus_tag="PLF85_02855"
     CDS             complement(14349..>14835)
                     /locus_tag="PLF85_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNE18626.1"
                     /translation="NQGKIQQVGRPIDVYNKPDNLFTAGFIGSPPMNFFELTVSREND
                     QVRVKNDAFSLSLAASHPLAQPLLKNFVGAPTLTAGIRAEHLKISRANTAKPSIHAQV
                     EVVEPAAPEVFLYLKSGEASFVMRDDTLLEPVVKPGETLGVYAEPEHIHFFDGNGQRL
                     C"
BASE COUNT         3079 a         3978 c         4290 g         3388 t
ORIGIN      
        1 gaattcacct ttccagaagc gtcgtgcggt atcgcgaaaa ctgtcgttcc attcaagcca
       61 cggctgtgga aaacgaccgc gctggtaacc gtcgggcccc aggtcccagg gttcggcaat
      121 caattttcgc tcgcgcagca ccgggtcacc cacgatcgca gcgtgcaatt cgtggtgaaa
      181 atccacggcg ccattcttgc gaaatatcgc cggcgccagg tcaaagcgaa atccgtcgac
      241 atgcatctct tctgcccagt agcgcaggct ctgcagcacg aggtcgcgcg tgtgcgcgtg
      301 gtcggtattg agcgtgttgc cgcagtgcgt gtagtcgatg tagctgccgt cttcagccct
      361 gcggtaatag acgccctcgg ccaggccgcg aaagctgaca gccggggcat cgtgcgcgcc
      421 ttcgcccgtg tggttgtaga ctacatcgag aatcacctcg atgccggcgc ggtgcagttc
      481 gcgaaccatc gtcttgaatt ccgtgacggc atcgcctgcc gcataaccgc tctcgggtgc
      541 aaagtaggcc agcgtattgt agccccagta attcgtgagt cccttcgagg taaggtaggg
      601 gtcttcgctc ttgccatgta cgggcagcag ttcaacgctg gtgactccca ggctcttgag
      661 atgcgccacc aggtgttcat ggtgcaatcc gccgtagcgt ccgcgcatct cgtcgggcat
      721 accgggaaat tcaatggtga gccccttgac gtgcgcctcg tagatgacca tctgcggtac
      781 gggaatctgc ggcagtctgt catcgcccca gtcaaaaccg ggttgtgaaa tttcggcgac
      841 cggggcaggc catgttttgc tgtcgcgcca gatatgcagc tggttcatgc tgcgcgcaaa
      901 cgggtctgcg aggtagagtg tttcaccctg gcgcgttact tcgtagcagt acaattcacc
      961 tgccaggtcg cgcacgacgc tgacctgcca gcgatcacct gccgcagtca gcggaatgcg
     1021 gtcgagcagc tggccttccc ggtcaaacag gcatagggta acctgctcgg cagcggtcac
     1081 cagcacggca aaaagccagg cgccgtcgcg gcgcgtgaca ccgtacggaa aaacgtctgc
     1141 agttgtttcc atcactgtcg catcagccgg aggtaagaag cagctgcttc gttccaggag
     1201 aagtcagtca ccatcgcgtt cgcctgcatc ttcagcaggg cctctggttc ttcattgtgc
     1261 agggctatga cgtggcgaat tttctgcagg aaggccgacg ccttttcagt gcgtgaggcg
     1321 ccatcgtaga gaaaaccgtt gtgttcattg atgatcgtgt tggcgagacc gccgacacgg
     1381 gcggcgattg gcacgcaacc gtagcgcatc gacttcatct ggctggtgcc gcacggctca
     1441 aaatcagaag gcatcagcat cgcgtcggca gcggcataga gccgtttctc gagattgtca
     1501 tcgaaaccgc agagaaagaa catattgccc gcaggtgcaa tttcgtggct catctgcggg
     1561 gccatatccc ctttgccgag aaagacataa tttgccccgg tctccatcag gtggcgcaga
     1621 acgcttttac cttctgcctc atcgaagaac tggcgtatct tttgcccggt catgcgcgtg
     1681 acggcgacca tgagtggttt cgtcatgaaa tgctgttcgt cggttcgccc gagccgggca
     1741 agagtgtctg caatactctc ttcagtgagt gactccgatt cacgaaagcc ggcaagtaag
     1801 tgggggagct cctgccagag cgcctcgcgg tttcgcaact tgcctgcggc ccggcttgcc
     1861 gccgagtacg catgcggcaa aactgccggg tcataaaaac tgaggtcgat gccattggta
     1921 atgccggtca ggcgaccggc aatgcgcagt tcacgaaaat ctttttcgag gccacgaccg
     1981 ccgaagaaac cctcgacggc attatcggcc cggcagatct cgtcggcata gttcggcgaa
     2041 accgtcgtga cctggtcggc cgagtgtatc gccgcgcgca ttgggttgat acagtcgata
     2101 ttggcgtacg ggtcgagata cggcgccatg gtttcccgcg gcagctgttc cagttcggga
     2161 aaccagtcga gaaaaccctt gaggtcgccc caggcggcat agggccgggt gccctggtat
     2221 tcaaggttgt gcacgctaaa gagacgaaag tacttcctga gctcggccag atcgggatgc
     2281 acgttgatca gcatgagcaa cagtcctgaa tgccagtcgt ggcaatggat cgtatcgatt
     2341 ttttctttgc gtgataatct caaaagcagc caggcagccg ctacggagaa gaaggcaaaa
     2401 cgtttggcat cgtcttcaaa gaaaccgcgc tgcaaacgcc cgctgtcgat atagacgtca
     2461 gaatattcac ccctgaaaaa gcggtcgctg tgaatgagca ggatcctgat tccctcgtgc
     2521 tcagaggcaa agaccgacac agtgctgatt tcgccgccgt aaggcacctt gagagtgagg
     2581 taactcttca cctcttcgcg gatttcttcg tagtagggca tcaccagcgt gactttgtgg
     2641 cccgactggc gaaaagcatc gctgatgccc caaacaacac cgcctaaccc gccgcgctgc
     2701 gccaggcccg aacactcgct gctgatttca aaaatgttca caaatggtaa ctggccccta
     2761 cgtagggcat cgcgcccagc atcacgttct tcgtcggcat cactatgacc tgcgtgcctg
     2821 cctcgatact gaccgagctc cccagccggt actggtagcc aaacgacagc gcgcccgtaa
     2881 tataggtgcc gaggtcgagc gactttggtg aaccagcgac ggagttctgg atgagcggaa
     2941 taccataacc cacttcgacg ccggcgaaga tgccgcgcag cgcttcgggc agaaattcgg
     3001 gaaagtagcg gaagccggcg ctcgccatga agaagctgac gacgctctcg gcattacccg
     3061 tcgaacggaa aatgctcgca ctgtaaaccg gcaggtacca aagcccgccc gtatattcga
     3121 tattgcccga gcgcacgcgc acatgcgccc cgcctgagac accgccgctg ttgtagttga
     3181 tcacgcactg ccggctggtg aggccgaggc agccatcggg gtctgatacc aggcggtcgg
     3241 caagccctgt cgtgagcggg ccaaactgga acatccagcc gccccagatg gtggtgtaga
     3301 tatcgtaacg cttcggcttc gtcgggtcga tttcgacgcc tttgtgtacc tgcgacaggc
     3361 gttcggtctg ctgcgccagc ggcggagtgt aaccttcttc ccagccactg acagagaacc
     3421 agtgctcgaa ctcgacgcgt accatgtggt gccagtgtat gtcgcggttg aggttgaact
     3481 ggttgcggcg gttaagactt tcttcaaagc tgttgccgaa tgccgcctgc acgttcgaat
     3541 gctgcacgcc gtacagcagg cgaatgaccg ggcgcgagta gataccgcgg cccgctgggt
     3601 tgatcgtgaa gcctgttttg atctggtacg tgtgcttcgt gtcggtgtcg ccccactcga
     3661 gaccttcact gtctggtata ccttgcgtgt ttgatttaat cgaattgaag tgctcgcggt
     3721 agcggttgcc gatgttcgat ttttcgcgcg cgtagaccag ttcaacgagg tagtgcaggt
     3781 gctcggtgat gtaccacatc gggcgaatca cgaaagaata ccgtgtgctg ttgtagtcgt
     3841 cggggcggag cttgttgtcg ttatcccagc ttttgccgaa accgaagccg gcgttcaggt
     3901 cgaaatggtt gggcaatagc cgcagctgta tttcatcgta caggctgagt tcataacgct
     3961 cgtcagtgaa agccgcgatg tagatgtctt tgctttcgtt gtcgtaggtc tcgcgtaccg
     4021 caatctgtgg cgcctttttt tcgaagcgta cgctcaggtc gttccagtag atcttgatcg
     4081 gtccgaggtc ggggccgcta aagccagtcc agccggtgta gcgccagtag ttcgtcgggt
     4141 gtggttgcgc ccaggggaaa aagtctgagt tgcccgggtt ctcgagcatg aatttcttga
     4201 aggcttcgcc gcgcatgaca tcggcgtagg ccacgttggg tgtctggtag ggtgcaccgc
     4261 gcatcgtttc gttagcgcgt tcccacatga gctggaagct cgcgccgacc tgccagcgcg
     4321 gcataaattt gttactgaaa ctcttcagtc cgtcgagctc atagaggttc agtttgacga
     4381 ggccgcccac ggtcggtatg accgcgtact ggtgtccctc ccacccatcg tagctgcgat
     4441 tacggtatag tgaccagcct gaatcgccgg cgcctaaaaa atactcaaag agcttcgtgc
     4501 gctggccggc acgaaagccg acagtatcat agaaaacctg cgcagtgaag ttatcaaaga
     4561 taccgatgta gcccatgttg taccacaggt taccggtctg cgtcaccagg gtcttgaaga
     4621 gtacgttctc cgtttcgagc cagacatggt tgatactgaa gttgctgagc gagccgttgc
     4681 ccatgttgta gccgcggtag ctgccgccct cgatacgcat gaacagcgtc gcacgcggag
     4741 ccccgggtgt ttcgggtctg accacgtggt agccgaagtc tgccatgagc cagggtgtct
     4801 cattcatcag tcggccatag aacggcgacc agcccagcgt gggtgcattg taaccgaagt
     4861 catcctgcgc actggcgcgt acgtacatgt taaagaagaa cggtttggga attttgaagc
     4921 cactcttgag ttcttcgagt tctgcgagac cgggcttctt ctctgcggta tcggcttttg
     4981 tctgggcggt cgcagctggt tgcgcgggcg tttcactagc ggctgccggc tgagggcggt
     5041 cgaggcgaat tttgccttcc tgtgcctgcg cccagacaat taccgaagag gcaactagcg
     5101 gtagcaccgt atatagacta actttctgca tctggcgact cgcgtagtta ctgtttctgc
     5161 aggatcagga tcgtgcgcgg cggcagcgaa taggttccgc ttgccgtcga ggcattgcac
     5221 ctgggcgccg aggggttaaa agtcgcttcg ccgctgttcg cgcagtaatt gccgctcaaa
     5281 ttcgcatgga agccttcgaa atagtattcg gtattggtca cgacattcca ggttcctgtc
     5341 ggcaggttaa tcgtatgcgt gttcactccg cccaagttaa agacaataaa aatttcggtc
     5401 ttacccgaag gtgggttctt gtaacatagt gcgattacct tgtcactgcc gccccaggcg
     5461 ccggagcccg caaaactcgc gttgcagtta ccgggtgaag caccaatgtc atgccagctc
     5521 aggtcaccca tgttgggata attattcttg ctgaacgccc acaggttatc tttccggaac
     5581 cggatgatct ttcgcgtaaa atcatacatg cggtatgcat acgggtcggc gagccagacg
     5641 ccccagcggt accagttgta ctggttgtca gagcccgtgg tgtaagcgtt attgttaccg
     5701 aactgcgtgc gcatccattc gtcaccgccc agcagcatcg gcgagccgtt agcgaccatg
     5761 agcagcacga gcagattgcg aaaattctgt cgcttgaggt tctcattgaa gccccagtcg
     5821 cgcgagtagt tactgccgtc gtcgcccgaa tacgggtcaa cgccatcagg attcagcggg
     5881 cccaggccgt tgcgcttctg gttgtagctg agcaggtcgt acatcgtgaa cccgtcgtgt
     5941 gccgtgatct tgttgatgtt gtggtacggc tgccggccgt cccaggtttc gttgaacttt
     6001 gcagagcttc cggtaagcgc cccgccaaag tccaccggcg agtcggagtt taaccggtag
     6061 tcatcgttgt tgataatccg ccggctgaca cgcttgaaca ggtcagacca ctcgtaccag
     6121 gcaaaacccg tcgtgtacgt ggtgtcgagc gcggtgcagc cagtttcgta gcccgcatac
     6181 ttatcgccat cagtgccgta gcagtaggcc ttggggtgat cgccaaaacg gtagccgccg
     6241 atcgaccagg gctcggcgat gatacgcgtg tcgtagcgtt gcagcaccgg gtcgttgaca
     6301 atccacattg aagcagacat attgcgcctt gaggtattgg tgctgctgta tgtttctgag
     6361 ccccattctt tccagtagtt gtacgagctc cagttgttgg tagcctggcc gtctttcttc
     6421 tcgagtactt cgctcaggtc aaaacgaaat ccgtcgacat gcatctgctc cacccagtag
     6481 cgcaggctgt cgatgaccat acgccgtacg ggcgggcttc cggtgcgcac catgttgccg
     6541 gtaccggtgt ggtcgtagta gaattcttta ttaggtccgg tgagcgcata gtagctgtag
     6601 ttgtcgatgc cgcggtacga caggatcgtt gccgtctctg ccgggtcata cgcccagaag
     6661 ttcgagggat cgttagggtt cacgggtaca ttgccctcaa tcagcggtac tttctcgcgc
     6721 cagagaccgc cttcacccgt gtggttatac acaacatcga tgatgacctc gatgttgtac
     6781 ttgtggaact gttcgaccat ccagcggaat tcgtttacct gtgaggcttt ttcggggcga
     6841 tacgcgtacg tgaattcggg agtaaaaaag ttgagcgtcc agtacttcca gtagccgcca
     6901 tcactacccg cctcatgaat gggtaagaat tctacggcgg tgatgcccag atcggcaaga
     6961 tacggcgcca gctcacctgt ccccctatac gtacccggaa aacgcgcgtg gtagtcggcc
     7021 ggcaagccgg gaatacccgc gagcgcagcg gctgaaatac cttttgggtg cacttcgtag
     7081 aaaacgagcg aattgcgcgc acgccgctgc gtcgttgact gcttgcgctg ccagtaatcc
     7141 tgctctgttg agctccagct gtagttgtca tcgcgcacga cgataccttt tgcggctgct
     7201 gcggttgtgt cctcgtgccg gtaagggccc gaggccgcca tacccttcga ccagtcgtgc
     7261 ttgcggtgta ctgctttcgc gtatgggtcg agcagcagtt tgttcgggtt gaagcggttg
     7321 ccctggttgt caaagtccga cttaaaacca aaaagtgtac cggggtacca tgaaggatgg
     7381 taatcgaaat tggggcccca gacgcggtag ccataatacg tgccgtggcc cacgccgttg
     7441 atgaacacgc tccagacatt gccgaagcgc gacatctgga attcctgcac ggggtacttg
     7501 ctgtcggggt tgtcaaagat cagcaggtgc acgcgcgttg cacgttcgga gaacagggtg
     7561 aagttcacgc cgccgtcgac gatcttcggg cccatcactt ccatattctt gatctgcgcg
     7621 gtgttgaacg acggcgcatg cggttcggcg tcgtacatac gaaagaagtc ggagttaggc
     7681 ccggcgcatt ttccaaagaa caatatgcac atgagcgctg cgcaaattgc tttttgtgga
     7741 tttcgcgttc tcattgtttc accagaatta ctgcgctgta gcaaggcacc gaccagctgc
     7801 tattcgttgt gccccagctg ttgatattat tgctgcccga tgcgtatgac cagcgcagcg
     7861 ttgccgccga ctggtcaccg cagttggtat tgtcgttacc gccgctgact gcggcgaccg
     7921 cgcgccagtt ggccccgccg gtttcgacag taaatgcctg cgccccgccc gtcatgttag
     7981 cgagaattct aaactccgac tttgtgttga tatatgtcga agagtcgccg ctgaagccag
     8041 aacaggcacc gcccttcgtg aaataagaaa ttgtctggtt aggtgtcgcg ccgtcgacgt
     8101 ttgagttgct gtttccgggg tctgacgcgt aacgcagtgc ggggcaggca cggcggagct
     8161 tggtcagaaa tgagacaaag ccgagcatgt cggcattggt cgccgcgagc tgccagtcga
     8221 ggttgttgcc tatcttgctt tggtcctgta tctgcttgtt gcgcatgaat tcctggcctt
     8281 cgtagagcat cggtatgccg tgtgcggtca gaagcagcag cgagccgatt gccgatttcg
     8341 atttcgagtc gagtaccgcg cgcgcgacgc tctcttcatc gtggctctca atgtagttga
     8401 gcgtatcgag cgggttattc gaaacaccct gccagacacc gtcgtcacgg cccttgctga
     8461 aatagatagc ggccttgctg cggttggtta cagttgcatc ggtattaccc tgtatgaagc
     8521 gcttgatgga ttcgcgaaag tagtcgttcc actgcgaaaa gccgcggtag tctgtgctga
     8581 gctgcgaggc gaagttgggc gtggtccagg tgctctgtac cataaagcca tgccgctggg
     8641 cgccgagcca gttctcgccg gtcatgaagt taggcagctt gatgatattg ttgaagttat
     8701 cccgggtatt gccatccact gtgacagtct cagtggcgct gctgcgcaga aattctgagt
     8761 cgatgagcat actggtttgc gtcgcggatg gaggtactgt tgttgtccat gcgcccggct
     8821 gcgcggcgtc attgatcata ttctggatat cgcgcaaggt ttgttcgctc gtgcccaccg
     8881 ccagatcaaa acgaaatccg tcgacgtggt aatgtttcac ccagtacttg atgttgtcga
     8941 gcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaacgaa atccgtcgac
     9061 gtggtaatgt ttcacccagt acttgatgtt gtcgagcacc agcttgtagg cgaacggccg
     9121 ctggttacgg tcgtcgaaga tttggttgcc tgtaccgacg ttcgaccagt tgagcccgtt
     9181 ggtgcgaaag taataccggc cgtaaggatc tgcctggtag agatagttga acgcattcga
     9241 agtatggttg agcacgacgt cgagaatgac cgcgatacct tgcgcatgca tcttcttgat
     9301 catgcgcttg agttcgagca cctggtagcc cgcgcgggca tcgacgtacg tgcaggcttc
     9361 agatgcattc gcgcgcgagc agtacgatga atacgacgac tccggtgcaa aaagcagcga
     9421 gggcaggtaa ccccagctgt agctttcggg attatctttg ttgtcgttcg atgactcgtg
     9481 cagcggcatc aggtgcaccg cgttgacgcc catggtagaa gacaggtgcg atgacgttac
     9541 cgtcggcgtg gtagtggcag ataccatggc ttcaaaaccg aggtacttac cagacaggtt
     9601 gtctccgctc gttgcgccaa ccgcagtcat cagtgcgacg ttgttcgggg tcgttgcgct
     9661 gtagtaagcc tgcagttgtg tcttgcaggt tgagccgctg tggctttgca ggcatgaagt
     9721 tgagccactg aagtcatcga cgtgtgcttc gtagacaatg tactggctac tcggcggccg
     9781 ggcggtgcca aaccagttgg cgccgccgat atccgaggtc actccatctg tcgcattgtt
     9841 ccagccgctg aagacagcat tcgcgagcca ctgggtgtcg acgatgatgc catgaccgtg
     9901 cgaaccagcg cttgcgagcg cgtacgggtc ggtgatataa cgatcgcgtt cggcaatggt
     9961 cttgttttcg tagttctgca tgcaattgat acggtagcgg tagtagtact tgcctgcgcc
    10021 cgcagcgctg acgggttttg cttctgcggt tgtaatggcc gcggtcgacc atacgccaaa
    10081 ccccgcgttg cccgtgccgt cgcgcgtcat gttgatgacg gcgctcgggc taaacatgct
    10141 atccgttgcc gcattgtaca ggacgacctc tacctgctgc gcggtagggg cgaagaagcg
    10201 gaacgtggta ttggtgccgt cgtagaccgc acccagtttc atatcgcgta ttgtcggcca
    10261 gtcagtggca gtgggttgtt tatagttgct tgctgcctgc ggattgctac aaggcgtatc
    10321 gatgacgtta ccctgtgcgt cggtgagatc gataaccgga atgtatgtgc ggggtttttg
    10381 ctctgagttg cagcgcatgg caaatgcggt catgagtaaa acagaaatga cagcagccgc
    10441 acgcctgcgc attggcgaga atcagttcgc gccttcagca agtaaagtat ttagttttgc
    10501 cggtatgacc ctgatcgtag agactatgac attcacccgc tattggtgtg gatgaatgag
    10561 acataaccca gatttgaaat ctgcctgcga ccacaccctc gacattcagg tcttcatcta
    10621 tttggcgcga gtaaatcacg ctcaatctca gcgcacgcac gccgcagtgc ttctttcgcg
    10681 ggcagtgagc cgtcgagcgc ggcgtagagg tacggggcga cgatggtttc gagcgcgtcg
    10741 taaccgggta gctgcggccg tgccccggca tagtcgagct gttgcagaaa gaccttaagc
    10801 tccgccgaaa gtttgccggc catcgcgcgg ttggccgccc ggttgacgct cagctgcccc
    10861 gtgccgctga tccatttctt ctgcacctct tgagatacca ggtatgccag cagtttgtac
    10921 gattcgtctt tttctttcga ggcagaaaag accaccatcg ccgcgccgcc gatattcgaa
    10981 gccgaatgca gcgacccgtt gccgggtacc aggctcacgg cgaacggtac cgtgcggaat
    11041 gtctgcaggt tccacgggcc cgagagaatc atcgcgtaac gaccagtcaa gaatccctgg
    11101 tcagggttta cagcgcccga cacccaggcg cctccttcgc tgccgctggc gtaaagagcg
    11161 cgcaggtatt cgacagcgct caccgcttcg gttgaatcga gcaggcagcg gtcgagatcg
    11221 cccgacaaga tctgcgcgtt gtgcaagaag agccatggca tcatccacca gagcgacgag
    11281 ttcatcgcga atgctttggc gtagttaccc tgttttcgga acagttcacc cattttgctg
    11341 agttcaccga ttgtgcgcgg cggtgtcaca cccgcggcgg cgagcagctt cgtgttgtag
    11401 tagagcgcaa ccgtcgtcag ctggtcgggt accgcgaaga tttcagggct cttgcgaccg
    11461 ggcaatttca cttcggcgac cttgcgtgcg accggcagca tatcgctcac aagtttttcg
    11521 gcgccgaatt caccgagtgg cgcaatcgcc ttgccccagg cgaggcgtgg tatgtgaccg
    11581 atgtcgacgc gtgcaatgtc aggcgtgcgg tgagcgatcg ccgccgaagt tagcttcggc
    11641 aaaagaccgt cgaatggcac gcgctcggca actatcttga tatgggggtt ttgtttctgg
    11701 tagtcggcga gcagatcgtt aaagactcgg gtttcttcat tattgtatga atgccagagg
    11761 gtgagggtgc gtggctcagc tggctttgtc ttagtgtcct ggctgcaggc gaggccaagt
    11821 aagataattg ccgctatgga aatccgtcgc aacactctat ttgcccccga attaattcgg
    11881 gggcaaatag agattggttt cgcagtcact ctttcactcc cccttgggtt agcccgctga
    11941 tgatataacg ctgcaagaac agaaagagca gtagtacggg gagcaccgag atcactgatg
    12001 cggccatcat cttttcccag gcgatcgaat actggccctt gaagagagag atgccgactg
    12061 gcaaggtgat ccacgagcta tcgggggtat tcacgatcaa ctgccacaaa aagttcgacc
    12121 actgcccgag aaacgtcagc agtactagtg tcaccaatac cggtgaagcc agcggtaaga
    12181 ttaccagtcg catcgtctgc cagtcgctcg ccccgtcgac ctcggctgcc tcaaagagcg
    12241 agcggggtat gcgctcgata gactggcgca aaaggaagat accgaaaacg ttcgccgtgt
    12301 gcggaatcac cagcgcctgc caggtgttca tccagccgaa ctcggcgatc agtgcaaact
    12361 gcggcaccat gaagatcatg cccggcacca tcattgatgc catcagcaga ccatagacca
    12421 gctctttgcc cacgaatgtc ttgcgcgcga aggcgtacgc cgacatgaag cagatcgcgg
    12481 ttgaaatcac ggctgtcgaa ccggccacgg ctgtgctgtt cagcagaaag cgaaagaacg
    12541 gaaaatccgg atcaccgagg atcgcgctga agtttttcca ggtgtagagt tcggcaaagc
    12601 ctgtgcgtgg gattatctgc gtgctcaggc ccatgccctc ttttttgagt gcagtcacga
    12661 acatccagaa gagcggcagc agggcaaaag cggcgagtgc tactatggaa atccatagca
    12721 aaatgagctt aagacgcctc atgcagtgcg ctcccgtaag aaccgccagt tcaggtatga
    12781 aacaatcagc gcgaagatga gcagaaagta cgacatcgcc gcagccgaac cgtagttcat
    12841 ttcggtgaag cgcgtataaa gcaggtagcc agaaacgcgt gtggcgccga cggtttcatt
    12901 ggcaaacagg gtaacgggat ttccttcggt catgatgtaa aattccgtga aggcattgag
    12961 gcagccgaga atgccggtga cggtcagaaa caggataacg ggcttcacca gtggcacgcg
    13021 aatgtacctg aacttctgcc accatgtcag gccatcgagg tcggctgcct caaaaatatt
    13081 cggtgagatg ttcttcagcg cagtcatgaa cagcaccata ccaaatccac aacctttgaa
    13141 gaccatggcg atcgcgaccg ctgcgagcgc agtatgcgcg tcacccagaa agcctttcgc
    13201 gtgaaagtat tcaactccca ttttcataaa tgccgtatcg agcaggccgt ccggactata
    13261 gatgagcttc cagacgaggc cgatcaccag catgtcgaga atgttgggca gaaacagcgc
    13321 cgagcgaaag aagcggctgc caggcaggtt ttcactgagt agggttgcga gtaagagaga
    13381 caaaaaaatg ccgggcggaa tcgtcagaag catgtaaagg aacgtcgcaa ccagcgacca
    13441 ccagaaatcc gcgcgcgtca aaagctcgcg gtagttctgc aggccgaccc agcgcatgtc
    13501 tgaaaaaact tcgtacaggt tggtctgcca ggtgactttc ttgaggctaa taatgaaaga
    13561 ataggccatc gggtaagcga gaaaaaccgc gaacactgcg agaaacgggg tgagccagag
    13621 gtagggggcg aagcggcgca ttcgcatgag agctgacaaa acggtgtgca ttggattact
    13681 ccttatcgca gcctaaacgc cttggtgtcg atgaggcgag atttcgcgag ttgctgatcg
    13741 ttcgtcagca gcacgcagcc attaattcgt gcggttgcaa tgataatcgc atcgggaagt
    13801 ttcagattat tgtcccgttt gattctgatc gtttcctgtt tgaccgagtc tgtcaaagtc
    13861 acaatttcaa ttgctttgag taaagtcttt atgccttttt cctcagcagt tgttaaacct
    13921 ggataagcca gtagttcgat ttctgaaatg atggagagga aatatcttcc ctcgggaagt
    13981 ctctctgcca gtcgattctg tagctggtag attgcaatat ttgtatcgac tggaaatttc
    14041 acgaccattc accgcggagg cgcttctggt attcaagtgc gtcctcacgt tcgcccagga
    14101 cgcctgcgag atcagcccat ggtggaacct tttcagtttt ctttctacga tgcttgggtt
    14161 tctgtttcgc tgatttggcc cgattggcct tcttggtttc ttgccagact tcatagggca
    14221 cgagaacgaa caacggttta cttgacctat cctttacgat gctatatgct ttcatcggcg
    14281 aagctgcagg tcggctccgg cgcacccctg tgtttttcag atttttcttc atggtttttc
    14341 tcctggtact agcataatct ctgcccatta ccatcaaaaa aatggatgtg ctcgggttcg
    14401 gcatagacgc ccagcgtttc acccggtttc accaccggtt cgagcaaagt gtcgtcgcgc
    14461 atgacgaacg acgcttcgcc gctcttgaga tagagaaaca cttcgggagc tgccggctcg
    14521 acgacttcga cctgcgcgtg tatcgaaggt ttagcagtgt tcgcgcggga gatcttgagg
    14581 tgctctgcgc gaatgccggc cgtcagggtg ggagctccca caaaattttt cagcaacggt
    14641 tgcgcgagcg ggtgcgacgc cgcgagcgat agcgagaagg cgtcattctt caccctgacc
    14701 tggtcgttct cgcgcgagac cgtgagctcg aaaaagttca tcggcgggct gccaatgaac
    14761 ccggcggtga aaagattatc aggtttgttg tagacatcga tcggtcgccc gacctgctga
    14821 atttttccct ggttg
//
LOCUS       DAOQJF010000030        14827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1929_length_14827_cov_6.373883, whole genome shotgun sequence.
ACCESSION   DAOQJF010000030 DAOQJF010000000
VERSION     DAOQJF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14827
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1929_length_14827_cov_6.373883"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLF85_02860"
     CDS             complement(<1..418)
                     /locus_tag="PLF85_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE18627.1"
                     /translation="MKYLHAMVRVRDLEKSLAFYRDALGLVENRRKDYPDGRFTLVYL
                     GAPGNRDAEVELTYNYDDEDYGGARNFGHLAFEVENIYEICERMQKLGITINRPPRDG
                     RMAFVRSPDAISIELLQKGAPLPPQEPWVSMPNVGKW"
     gene            complement(425..811)
                     /locus_tag="PLF85_02865"
     CDS             complement(425..811)
                     /locus_tag="PLF85_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18628.1"
                     /translation="MKRILITVLSIYLFVTAAHADQFAYIKPETARQAVELLAKEKEV
                     YFFCEPCNESTGQLTKIDKFEAVDVNYEGFHEVQINGQGIDLAYTYVMRKGRWKNVAV
                     ELGLKPTGVSAEIQPIKPSRPQTAAY"
     gene            complement(862..1353)
                     /locus_tag="PLF85_02870"
     CDS             complement(862..1353)
                     /locus_tag="PLF85_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE18629.1"
                     /translation="MTSNTFSGSMGGVYGADNFCRFDPANPAGTGRGNWKAMLTSNNP
                     VRRACLSAGCAVAGAAENIDWVLRPNTPYKRADGAPVGTTSGNGIFQTTLTNSIGVIG
                     VGVWTGLTLDWENNSLNCGNWFDGTSGPEGSIGTATGVINDAIASGTSFCNQGNYLYC
                     VEQ"
     gene            complement(1590..2456)
                     /locus_tag="PLF85_02875"
     CDS             complement(1590..2456)
                     /locus_tag="PLF85_02875"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE18630.1"
                     /translation="MATKFTVKESSPLIDFLLLKLHGKSRTGVKSILARGIVSVDGAI
                     VTQAKHSLLPGQVVTIGDAAPEKPVTLRGVKIVFEDDSVIVIDKAAGLLSVATDTGHE
                     RSAHSIVSEHVKRKTPKAKVLVVHRLDRDTSGLMMFVKDRELQRQLRQNWQQSIETRR
                     YIVLVEGKVAKESGTITSWLKGTDSLRTYSSKTPNDGQKAISHFQVLQRSDEYTLLSV
                     ELETGRKNQIRVHMQDIGHPVVGDEKYGAQSDPIDRLGLHAAELTFEHPRTGKLLVLK
                     SEPPKEFLKIFS"
     gene            2519..2794
                     /locus_tag="PLF85_02880"
     CDS             2519..2794
                     /locus_tag="PLF85_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18631.1"
                     /translation="MTNNRVWQSLLLAALMVIGGCRSVDRATEMCEQNNRMCHQNCNL
                     ANTSMSHSKMAHQSVSQCDLRCEKNYQSCLKRAEDRSVRIIDDTPEK"
     gene            complement(2803..3627)
                     /locus_tag="PLF85_02885"
     CDS             complement(2803..3627)
                     /locus_tag="PLF85_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE18632.1"
                     /translation="MKPGAKISLVALGALAALVILTMSLRGTLTRPFIFPAPNYRFTP
                     ALPGGKMLSVRGERTAYGYYAKAGKRLVVFFHGNGEVMGSMQDLAAMMLAQGMSVLLA
                     EYPGYGYAAAHTASEANLYADSQRLLETVQRDYQHRKEDTLLWGFSLGTGVAVEMAAR
                     GLGGSALLMAPFTSMVELAEKHFTPLARLLVVDRFDSAAKASTIQLPVLLVHGDRDQV
                     IPISQARSLAGLFKNSQLLEVAGADHNDLAALMSKEAWKSVTEFALKGSLPAAKSR"
     gene            complement(3624..3836)
                     /locus_tag="PLF85_02890"
     CDS             complement(3624..3836)
                     /locus_tag="PLF85_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18633.1"
                     /translation="MPVLRAAFSLPVADAQRTERFYSGIFAAEMVSREAGGEPCGQAV
                     PYPWGLAAYFKDPDGHLWEVLWHSGK"
     gene            complement(3892..4170)
                     /locus_tag="PLF85_02895"
     CDS             complement(3892..4170)
                     /locus_tag="PLF85_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18634.1"
                     /translation="MPDYASFFRDDRPDSAYNVLITELHFLVQEDDTEGGFVARAVGE
                     SITTQADTEAELKEAIRDAVLCHFEVGKAPKIIRLHYAREEVLRLLDL"
     gene            complement(4254..4538)
                     /locus_tag="PLF85_02900"
     CDS             complement(4254..4538)
                     /locus_tag="PLF85_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18635.1"
                     /translation="MKKTILAVTALVFVGALASVEVVPSEAKLTNCENLGKVQAGDRF
                     AGLDRETAIKAVKEEAQAMNANKVFLDVTSYNHPKLGKQYVAKASAWKCN"
     assembly_gap    5118..5217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5218..5402)
                     /locus_tag="PLF85_02905"
     CDS             complement(<5218..5402)
                     /locus_tag="PLF85_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18636.1"
                     /translation="MTQRVQKWLSAGKYLAVDGLQIYYQEAGSGPADPNSGRHMAERY
                     QQELPQSAVYLLSEQIG"
     gene            complement(5419..5811)
                     /locus_tag="PLF85_02910"
     CDS             complement(5419..5811)
                     /locus_tag="PLF85_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18637.1"
                     /translation="MKHFRKRIFSPERIFLVDGLGAAYSATMLGVVLAAFVPFFGMPT
                     TVLYPLCTAASSFAVYSLSCHLIRPRRWQIFLRGIAILNLAYCVASMVLMSIYWNSLT
                     IWGAAYFVSEKFVVIGLALQELSLSRKS"
     gene            complement(5808..6434)
                     /locus_tag="PLF85_02915"
     CDS             complement(5808..6434)
                     /locus_tag="PLF85_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNE18638.1"
                     /translation="MGISERSGQRIAGILFLCAFLAYGLGTSIVAPLIEAGNATAVLL
                     SARGQLIAGCALMLSNSVIVIAIGLLLRPALAGANSQTASTYVTARVIEGLALAVGAF
                     ALMSLAFSGDALSLSHLTSAKRVNFIGYQAGMLALGLGSLPFCLLLFRQRFVPVWLAV
                     WGLMGYAIFATGALLELFGLPYGIALSVPGGLFELVFGVWLMVKGFKL"
     gene            complement(6421..7422)
                     /locus_tag="PLF85_02920"
     CDS             complement(6421..7422)
                     /locus_tag="PLF85_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE18639.1"
                     /translation="MRHNSERRMRAAVCTAYGAPEVLQVTEVPVPTPGPTDILVRVHA
                     SAVNSADWRLRRPDPWGVRLFLGLRKPRIKVLGGVFAGEVVATGSKVTRFRSGDKLFG
                     STLMRFGGYGEYVALPEKAVMTTIPCGFSFNDAAALSFGYLTALHYLRKAKIVPGQSV
                     LVVGASGSVGTAAVQLAVHFGANVTGVSSKANLDLVRSIGAEQTLDYNSDEYAKEATQ
                     YDVVVDTVGKVPWKDHLHRVKADGKLVLINPGALRSMYLPLLQPFYKPRIVAGVGAEK
                     RVDLEIIRDLAEQKKLRPVIDRTYPLAQIAEAHRYVEAGHKKGNVVVTVARGTNGNI"
     gene            complement(7502..7702)
                     /locus_tag="PLF85_02925"
     CDS             complement(7502..7702)
                     /locus_tag="PLF85_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18640.1"
                     /translation="MKIQTIQQNKLEEFKVNIIIATVNIPFLLFNLAGVAWIHMVTGA
                     VVQVILSGLIIYYAWKWPRAET"
     gene            complement(7744..8415)
                     /locus_tag="PLF85_02930"
     CDS             complement(7744..8415)
                     /locus_tag="PLF85_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18641.1"
                     /translation="MRGIARGEKMNLRRILLLFLAVSAAAYSQPVEARPANSEEPFYR
                     YFVGSTLLMLGNFIPDDPNRPDFAQVNLGYRITPKNVVFLELKTSKFNWPLGMPWPEL
                     VDEQRQQYNFPGYVRQYIIGMAYNHFWWKGLYTGVHAMHAYQVYNTEDSTEIATGYTL
                     FLTYRLGYQFRFFDDLFFIEPSLGLTHWPIKTNTPESFATKEAKWPKYFGFEPGLHFG
                     FNFNF"
     gene            complement(8470..9129)
                     /locus_tag="PLF85_02935"
     CDS             complement(8470..9129)
                     /locus_tag="PLF85_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18642.1"
                     /translation="MIRMRLIAVLIGALTLLGANATFAQKQAQTVAVVPNEAQAGAEP
                     FNRFFVGTSLFMAMNAVLDPSPGFYQLNAGYWLTKKDVLSVEAITWAYHRPLGTPWGP
                     DMSDPAQAYPGYVRAYGVGVAYQRYLWQGLYAAVHALPLLQQFKTPDGKTIQEGFQLF
                     MTLRLGYHFSFCDDHVFIEPSIAVTQWPVNTNVPADFARFDDRWPGYFIEPGLHVGFK
                     F"
     gene            9320..9997
                     /locus_tag="PLF85_02940"
     CDS             9320..9997
                     /locus_tag="PLF85_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE18643.1"
                     /translation="MPKKQKSENSPLRKPLSRERILKAALALTDKDGIESLSMRNLAA
                     ALGVEAMSLYKHVANKDAVLDGLVELILREMEIPPAGTPWREAMRRRAWSVRHTILRH
                     PWASALMESRLAPGEARLSYADRVLSILKSAGFSTQAQYRAFQLLDSYVYGYVLQEVN
                     WPTKESDRKMLVVRLKEFLHDGRYPNLVSVIEQASWLKVAPGEKPYDADFQFGLELIL
                     DGIERLL"
     gene            10057..11079
                     /locus_tag="PLF85_02945"
     CDS             10057..11079
                     /locus_tag="PLF85_02945"
                     /inference="COORDINATES: protein motif:HMM:NF027219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNE18644.1"
                     /translation="MPRSPIDLENLDEKQLMELRLCDLPLKIENTWLGELVEQLYREL
                     ESRALAFRPPCYLGDEWFSPEGVPAIALPFYLAHPRLIQIERKLIGEAEGESRDEAMR
                     LLRHECGHALVHAFNLTKRASWREVFGHPRKPFSDYYRYQPYSRSYVRNLKDFYAQSH
                     PEEDFAETFAVWLNPDINWRELYAGWPALRKLEYIEELVPAIGNKTVSVAKSEFFWHI
                     STLKKKLDSHYKAKRKLYIEHEADFFDADLKQLFPAPQGKSKIAAARFLAGQTSDLER
                     TVSRWTGEKKVLINNLLARLKKRCQVLKLEVNARGTDAALVEFTAYLTSLVSNHRYTN
                     RYKPGK"
     gene            11082..12155
                     /locus_tag="PLF85_02950"
     CDS             11082..12155
                     /locus_tag="PLF85_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE18645.1"
                     /translation="MASADNSDNAQAERAKERRKRQKKLKILVAFDYPLEPPKDGNYA
                     AQLHADDWRPIRNVTEAISRLGHEVILQGVYDNIMPLIYTIKRRKPDLAFNLLESFKQ
                     QRKYEGALAGLFDLLQVPYTGCPSEALTICRNKQYTKQLLSQYDIRIPRSVLFPRGKT
                     NRSVKDLAFPLIVKPLGLEGSDGISQASFVEDEKALIERVRTLHETYEVDALAEEYIE
                     GREIYAGVLGLDKLTALPLREMLFTNFPEDKPKFATFHAKWNPEFRKKWGIKNTFAKG
                     IDEKVEAEIENVSKTAFRALGLSGFARLDLRLTDKNEIYVIEVNPNPNIANDDEIAYA
                     AQRLGISYDQLIQRLIDYGRRKV"
     gene            12371..12910
                     /locus_tag="PLF85_02955"
     CDS             12371..12910
                     /locus_tag="PLF85_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE18646.1"
                     /translation="MKVQQRIKHFHTILFLLALSTVAVACGSTDKKADSNAAGATGAS
                     GASSRSFGGDMVVDAANDQLAKVPVVGFPPFSNTMPMTQFDQYGENAATVAKGVVNNM
                     PDGYVLQIKGHANQHPSKSAAYTKSLSLQRAKYVYNYFAGKGIDKKKMTAVGVGNAEP
                     DPSLSHAENRRVTFKLVPK"
     gene            complement(12968..13720)
                     /locus_tag="PLF85_02960"
     CDS             complement(12968..13720)
                     /locus_tag="PLF85_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE18647.1"
                     /translation="MNGFLLAAGRGTRLKHLTEQTAKPALPVAGLPMLAYSLSFFYHA
                     KVKKLAVNTHHCAESINQIFARAETPWQLHLSHETELLDTGGGVKKCEGLLDDDTVLL
                     ANGDVVCDVDAVKLITLHRAQQAALTLVVVPHPQANAMAPITIDDKGHITDINRTFDK
                     DSTGNHLYAGIAVFEPELFAFLKSEPSSIVYTGYTGLIAAGKKVCAYVHEGLWYDCGT
                     PESYAQVNAEVSGNLAHWKKTLHPAVKKLALS"
     gene            13856..>14827
                     /locus_tag="PLF85_02965"
     CDS             13856..>14827
                     /locus_tag="PLF85_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18648.1"
                     /translation="MQCSDCLEFQNGLLIQLTMQTDPATILLLTAIVAIVTGVILWAF
                     ELTQKPGIPHLRMWAYGTLLQGTGLLVVMGLRTAVPYQLGILSGNMLLMSAVLLYGSV
                     IAAYTARTIPRSFWFLLLLLFAASLSVYLYLWPNLRARVAIFSVFAAFVLFASALWLY
                     SSASNKNPSERFVAFLFAFTGLGMTLRAAVQYPFEPTTQDLLYSNSAYAGVFHMLALV
                     STLVSTLGVTSLCIERSRSESQEAQREAAAQREIFRRQFEMLPVAACIWRIEGNDYVL
                     EQANFAAQVLTEGRIWTQTGGLASQLLARHPEAIEGLNRCRNQLDYFV"
BASE COUNT         3313 a         4019 c         3996 g         3399 t
ORIGIN      
        1 accacttgcc cacattcggc atcgaaaccc acggctcctg cgggggcagt ggtgcgccct
       61 tctgcagcag ctcaatagag atggcgtcgg gtgagcggac gaacgccatg cggccgtcgc
      121 gtggtggccg gttgatcgtg ataccgagtt tctgcatccg ctcgcaaatc tcgtagatgt
      181 tttctacttc aaaggcgagg tggccgaagt tgcgcgcgcc gccgtagtct tcatcgtcgt
      241 agttatacgt cagctcgact tcggcatcgc ggtttccggg agctccaagg tagacgaggg
      301 taaaacgccc gtcgggataa tctttgcgcc ggttttctac aagaccgagc gcatcacggt
      361 agaaggcgag ggatttttcc aggtcacgga cacggaccat tgcatgcagg tatttcacag
      421 tcgttcagta agctgccgtc tgcgggcggc tcggtttgat gggctgaatt tccgcgctga
      481 caccggtggg tttgaggccg agttcgactg ccacattctt ccagcgacct ttacgcatga
      541 cgtaggtgta tgccaggtcg attccctggc cgttgatctg tacttcgtga aaaccttcgt
      601 agttcacatc gacggcttcg aatttatcaa tctttgtgag ctggccggtc gactcgttgc
      661 atggttcgca gaaaaagtag acctccttct ccttcgctag cagctccaca gcctggcggg
      721 cagtctcagg cttgatgtag gcaaactggt ctgcgtgcgc agccgtcacg aacaggtaaa
      781 tgctaagtac agtaatcaaa atccgtttca tgactgcacc ttgctgataa cgaattgctg
      841 cgtcgagttt ttcgtcagtt actactgctc aacgcaatag agatagttac cctgattgca
      901 gaaagaagta cccgatgcta ttgcatcatt aataacaccg gttgcagtgc caatacttcc
      961 ctctggcccc gaggtgccat cgaaccagtt accgcagttg aggctgttgt tctcccaatc
     1021 cagggtcagg ccggtccaga cacccacccc gataactcca atcgaatttg tcagagtcgt
     1081 ctggaagatt ccatttccgc tggtcgtccc tacgggcgcg ccgtcggcgc gcttatacgg
     1141 cgtattggga cgtagcaccc aatcgatatt ttctgcggca cctgcgactg cacagcctgc
     1201 gctcaggcag gcccgccgca cggggttatt ggacgtcaac atcgctttcc agtttccgcg
     1261 gcctgttccg gcagggtttg ccggatcaaa gcggcaaaag ttgtcggcgc cataaacacc
     1321 gcccatactc cctgaaaagg tattggacgt cataaatatt ctgcaatctg aaccgcaggt
     1381 tgactttaca gaaccaggat tttccaattg ctgcagcagg tcgtagttgt tgcaactcat
     1441 tgtgagtgta gcgatgaaca acccggcaac cagtaatgct gttctcttga taagcatgcg
     1501 ttgaatttgt accggcggag tgtcatgtaa agtgcaaaca ctctacaggc tgagactttt
     1561 ccctgccggc agcgaaactt ttaggagagt caggaaaaaa ttttcagaaa ctctttcggt
     1621 ggttcagact taagcaccag caacttgccc gtacgcgggt gctcgaaggt cagctcagcg
     1681 gcgtgcagac ccagccgatc aatcggatcg ctttgagccc catatttttc gtcgcccacc
     1741 accgggtggc cgatatcctg catgtgtacg cgaatctggt tcttgcgacc tgtttcgagc
     1801 tcaaccgaaa gcagggtata ctcatcgctg cgctgcagta cctggaaatg cgagatcgcc
     1861 ttttggccgt cgtttggtgt cttcgacgaa tacgtgcgca ggctgtcggt gcccttgagc
     1921 cacgaggtga tggtcccgct ctctttggca accttgccct cgaccagcac aatgtaacgc
     1981 cgcgtctcga tgctctgctg ccagttctgc cgcagctggc gctgcagctc gcggtctttg
     2041 acaaacatca tcaggcccga ggtatcgcgg tcgagccggt gcacgacgag taccttcgcc
     2101 ttcggtgtct tgcgcttcac gtgctcgctg acgatgctgt gcgccgagcg ctcgtggccc
     2161 gtatcggtcg cgaccgaaag caggcccgca gccttatcga tgacaatcac cgagtcgtct
     2221 tcaaagacaa ttttgacgcc gcgcagggta acgggctttt cgggcgctgc gtccccaatg
     2281 gtgacaacct gcccgggcaa gagggagtgt tttgcctgcg tgactatggc gccgtctacg
     2341 ctgacaatcc cccttgcaag aatactcttc acgccggtgc gacttttacc gtgcaacttg
     2401 agcagcagga aatcgatcag cggcgaagac tctttgacag taaatttggt cgccattttg
     2461 gtcattgtgg acagcgaaac ggcttgtcga ccgcgatcag gttttgcaga ataagcctat
     2521 gactaacaat cgtgtctggc aaagcctgtt gcttgccgct ctgatggtaa tcggcggctg
     2581 ccgctctgtc gatcgcgcga ccgaaatgtg cgagcagaat aaccgcatgt gccaccagaa
     2641 ctgcaatctg gccaatacca gcatgtcaca ttcgaagatg gctcaccaga gcgtcagcca
     2701 gtgcgacctg cgctgtgaaa agaactacca gtcatgcctc aagcgggcag aagaccgctc
     2761 ggtgcgcatt atcgacgata cacccgaaaa ataaaatgcc gcttacctgc tttttgcagc
     2821 aggcaggctg cccttcagcg caaattcagt tacactcttc catgcttctt tagacatcag
     2881 cgccgccagg tcgttatggt cggcgccagc aacttccagc agttgtgagt ttttgaaaag
     2941 ccccgcaagg cttcgagcct gcgagatagg tatgacctgg tcgcggtcgc catgcaccag
     3001 cagcacgggc agttgaatcg tgctggcttt ggcagcgctg tcgaaacgat cgactaccag
     3061 caagcgcgcg agcggagtga aatgtttttc tgccagctcg accatcgagg taaacggcgc
     3121 catcagcagc gccgaaccac cgaggccgcg cgcggccatt tcgactgcca cacccgtacc
     3181 gagagaaaat ccccagagca gagtatcttc tttacggtgc tggtaatcgc gctgcacagt
     3241 ttcgagcagc cgctgcgagt ctgcgtagag gttggcttca ctggctgtgt gcgcggctgc
     3301 ataaccgtaa ccggggtact ccgccaaaag cacactcatg ccctgcgcca gcatcatcgc
     3361 cgcgagatct tgcatcgaac ccatcacttc gccgttgccg tgaaaaaaca cgacgagccg
     3421 tttgccggcc ttggcataat aaccataggc ggtgcgctcg ccgcgcacag acaacatttt
     3481 accgccgggc aacgcggggg tgaaacggta gttcggcgca ggaaaaataa agggacgcgt
     3541 gagtgtgccg cgcagggaca tcgtaagaat aactaacgcg gcaagagcgc ccagtgcgac
     3601 cagagaaatc ttcgcgcccg gtttcatttg cccgaatgcc acagaacctc ccacagatgc
     3661 ccgtcggggt ctttgaagta cgccgcaagc ccccacgggt acggcactgc ctgcccgcag
     3721 ggttcgccgc cggcttcgcg gctgaccatc tctgcagcga agatcccgct gtagaagcgc
     3781 tcggtgcgct gtgcatcggc gactggcagg gagaaggctg cgcgcagcac gggcatggct
     3841 tgaggttgtg ggcgggtaac tgtgcgacaa gtggaatttg gcaggtactc gctaaagatc
     3901 cagaagtcta agaacttctt cgcgcgcata gtgcaggcga ataattttcg gtgctttccc
     3961 aacttcaaaa tgacagagaa ctgcgtcacg aatagcttcc ttcagctcgg cttccgtatc
     4021 agcctgggta gtaatggatt ctccgacggc cctcgcgaca aaaccaccct cggtatcgtc
     4081 ctcttgcacc agaaaatgca gttctgttat aagtacatta taggcggaat ctggccggtc
     4141 gtcacggaag aaactcgcat aatcaggcac ctaccgcctg catccgcgcc cgttgtgaaa
     4201 agagaaacac ccgcctcaac aagaggcggg tgctatcgac ttgctagcag caattagttg
     4261 cacttccagg cgcttgcctt ggccacgtat tgcttaccga gtttcgggtg gttgtaagaa
     4321 gtaacatcca ggaacacctt gttcgcgttc atagcctgcg cttcttcttt cacggctttg
     4381 attgcagttt cgcgatcgag gcctgcgaag cggtcaccag cctgtacttt accgaggttt
     4441 tcgcagttgg tcaattttgc ttccgagggc actacttcta ccgaagccag ggctcctaca
     4501 aacaccagtg ccgttacagc gagaattgtc tttttcatgt tgtcctccaa tgatacacga
     4561 agtatcttgg gtgcgacgga cacgaggtcc ttttttgtcg cacccttgtc ggaagggggg
     4621 cttccccttt cgatgatcag aacttagcac atatctgcgc gtttgtcatc agcgccgaca
     4681 ccgtaaaatc tgcgtggtac tacggagttt tggggctata gcgcctgcag gacttggggt
     4741 tctgtttgct tctttcgttt tcaggtcatt caagggtatt tctaacacgc gcgccgactt
     4801 gcagaatgga gtgaaactga ctttcgagga aaagatcgcc gggaaaaaaa ccctgactca
     4861 aaacattcct tcgggtcagc caatccctac gagcgctacg tcgtatttaa ttgtagtgag
     4921 aggtgtaacc tgtgatgtcc ccaaagattt ttttgacacg gttgccgtcg gcgacccggt
     4981 ggttctgagc attgcacccc tcagccgaat ctgcttcagc gtacgaaagt ctggacctct
     5041 gtcataactg cctattgttc tgctgcgctc aagcagagag cgcatggatt tcttgacaag
     5101 cggtgtttgc gccgcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg
     5221 cctatctgct cactgagcaa atacaccgct gactgcggca gctcctgctg gtagcgctca
     5281 gccatgtgtc ggcccgagtt ggggtctgcc ggcccgctgc cggcttcctg gtagtagatc
     5341 tgcaggccgt cgactgcgag gtacttgcct gcactgagcc atttctgtac acgttgcgtc
     5401 atagttactc cttgtcggtc atgattttcg ggagagtgaa agctcctgca aagcaagacc
     5461 gatcaccacg aacttttctg agacaaaata ggctgcaccc cagatggtca gtgaattcca
     5521 atagatagac atgagtacca tcgaagccac gcaataagcg agattcaata tcgcaatgcc
     5581 ccgaagaaaa atctgccatc gccgtggcct gatgaggtgg cagctgagtg aatagaccgc
     5641 gaagctggaa gccgcagtgc agagcgggta cagcactgtg gttggcatgc cgaagaatgg
     5701 tacaaatgcc gcgagtacga cacccagcat cgtggcagag taggcggctc ccaacccgtc
     5761 gacgagaaag atgcgttcgg gtgaaaagat tctctttctg aaatgtttca tagtttgaat
     5821 ccttttacca tcagccagac accgaatacc aattcgaaca gaccacccgg tacagagagc
     5881 gcgataccgt atggcaggcc gaataattcg agtagcgcgc ccgtggcgaa aatggcgtaa
     5941 cccataagac cccagacggc gagccaaacc ggtacaaatc tctgcctaaa gagcaaaagg
     6001 cagaacggca aactgcctag gcccagtgcc agcatacctg cctggtaacc gatgaagttt
     6061 acccttttcg ccgatgtcag gtgagaaagc gaaagtgcat ctcctgagaa ggcgagagac
     6121 atgagcgcga aagctcccac tgcgagtgca agaccttcaa tgacgcgcgc agtcacgtac
     6181 gtgctggcag tttggctgtt tgcgcccgcg agcgcgggtc tcagcagcag gccgatcgca
     6241 atcacaatca ctgaattgct cagcatcagg gcgcaacctg caatcagttg gccgcgcgcg
     6301 ctgagcaaga ctgcggtcgc gttgcctgcc tcgatgagcg gagcgacgat cgatgtacca
     6361 agaccgtagg ccaagaacgc gcacagaaaa agtatgcccg cgatgcgctg ccctgaccgt
     6421 tcagatattc ccatttgttc ctcttgcgac tgtgacgacg acattgccct ttttgtgacc
     6481 agcttcgacg tagcgatgtg cttcggctat ctgtgcgagc gggtaggtgc ggtcaatcac
     6541 ggggcggagc ttcttctgtt ctgcaaggtc gcggataatt tccagatcga cccttttttc
     6601 tgcgccaaca cccgcgacaa ttcggggttt atagaatggc tgaagcagcg gcaggtacat
     6661 acttctcagt gcgcccggat tgatgagtac cagtttgcca tcggctttta cgcggtgcag
     6721 gtgatctttc catggcacct tgcccacggt atcgacgacg acgtcatact gcgttgcttc
     6781 tttcgcgtat tcgtcactgt tataatccag agtttgttcg gcgccaatgc tgcgcaccaa
     6841 gtcaagattc gccttgctcg aaacacccgt gacatttgcc ccgaagtgca cggccagctg
     6901 cactgcggcg gtgccgaccg aacccgaagc gccgacgacg agaacgctct gtcccggtac
     6961 gatcttcgct ttgcgcaggt agtgcagcgc tgtcaggtaa ccaaacgaca gagccgctgc
     7021 atcgttgaag ctgaaaccgc acggtatggt tgtcatgact gccttctctg gcagcgcgac
     7081 gtattcgccg tacccgccaa agcgcatcag cgtcgaaccg aagagtttgt cgccagaacg
     7141 aaagcgggtc acttttgagc cggtagccac tacctcgccg gcgaaaactc cgcctaagac
     7201 cttgatgcgc ggtttgcgta agcccaaaaa gaggcgcaca ccccaggggt cgggacgcct
     7261 gaggcgccag tcggcagagt tgacggcgct cgcgtgcacg cgcacgagta tgtcggtcgg
     7321 gccaggtgtc ggcacgggca cttcagtcac ctgcagtacc tcgggtgcgc cgtaggcggt
     7381 gcacacggct gcgcgcattc tgcgttcgct gttgtgtctc atagttgtcg caccaaaagc
     7441 ttacagtgta agctcgtcaa gcagaatcgc tgaacccggt ggtacacccg ggtaagattc
     7501 ttcatgtttc ggcacgaggc catttccatg cataatagat aatcaaacca gaaagaataa
     7561 cctgtacaac tgcgccagtc accatatgaa tccaggctac tccggctaaa ttaaataaca
     7621 agaagggaat attaaccgtg gcgatgatga tattgacctt gaactcttcg agtttgtttt
     7681 gttgtattgt ttgtattttc atattttcct ctattttatc tatagcatga tttgatcggt
     7741 atttcaaaag ttaaaattaa agccaaaatg gagtccaggt tcgaaaccaa agtattttgg
     7801 ccactttgct tcttttgtgg caaaagattc tggtgtattc gtttttatgg gccaatgcgt
     7861 caggccgagc gatggctcaa taaagaacag gtcgtcgaaa aatctaaact ggtaacccag
     7921 gcgataggtc agaaatagcg tatagccggt tgcgatttct gtgctgtctt cggtattgta
     7981 tacctggtaa gcatgcatcg catggacacc cgtatataag cctttccacc aaaaatgatt
     8041 gtacgccata ccaatgatgt attggcgcac atagcccgga aaattgtact gctgccgttg
     8101 ttcgtctacc aattcaggcc atggcatgcc caacggccaa ttgaacttgg atgttttaag
     8161 ttctaagaag acaacgttct tcggtgttat ccgatagcca agattaacct gtgcgaaatc
     8221 cggtcgattc gggtcatcgg gaatgaaatt tcccagcatc aacagcgtac taccgacaaa
     8281 gtagcgataa aaaggctcct cgctgttggc cggcctggcc tcaacaggtt gactataggc
     8341 cgcggctgat accgctaaga acaataacaa tattctccgt aaattcatct tttctcctcg
     8401 cgcaatgccg cgcattcaag accccaatgg cttacagtgt aagccattgg gtcaaaaaaa
     8461 tgcacattct caaaacttaa atcccacatg cagtcccggc tctatgaaat aaccgggcca
     8521 acggtcgtcg aagcgtgcaa agtctgcagg cacgttggtg ttcaccggcc attgggtgac
     8581 ggctatcgag ggttcgatga aaacgtggtc gtcgcagaag ctgaagtggt aaccgaggcg
     8641 cagcgtcatg aagagctgaa aaccttcctg gatagtctta ccgtcgggcg ttttgaattg
     8701 ctgcagcagt ggtaatgcgt gcaccgctgc gtacagaccc tgccacaggt agcgctggta
     8761 ggcaacaccc acgccatagg cgcgcacgta accagggtac gcctgcgccg gatcgctcat
     8821 gtcaggaccc catggagtac cgagcgggcg gtggtatgcc caggtgattg cctcaaccga
     8881 aagcacatct tttttcgtca gccaataacc cgcgttgagc tggtagaagc cgggtgacgg
     8941 atccagcacg gcattcatcg ccataaaaag tgaagtaccc acgaaaaagc gattgaatgg
     9001 ctcagcgccc gcttgtgcct cgtttggtac aacagcaaca gtctgtgcct gtttttgggc
     9061 aaacgtagcg ttagcaccga gcaatgtgag tgcaccgatg agcaccgcta ttaatctcat
     9121 tcttatcatt tgttctcctc gccgcccgga tgcgaaaccg tgtcgattcg ggcagcgagg
     9181 tcgcaacggc ttacagtgta agcctgtaaa gcggaatatg gtttttttgc ctctgccctg
     9241 cccgaagggc agggcagagg ctctttactt gacgcgctgc acggatggaa aaaggttaca
     9301 ctgtaagctg ccacaaaccg tgccaaagaa acagaaaagc gaaaactccc ccttgcgcaa
     9361 accacttagt cgcgagcgca ttctgaaggc agcgcttgcg ctgaccgaca aagacggcat
     9421 tgaatcgctg tcgatgcgta accttgctgc agccctcggc gtcgaggcga tgtcgctcta
     9481 caagcacgtg gcaaacaaag atgcggttct cgacggtctc gttgaactga ttttgcgcga
     9541 gatggagatt ccccccgctg gtacaccgtg gcgcgaggcg atgcggcggc gcgcctggtc
     9601 ggtgcggcac actattttgc gtcacccctg ggcctcggcg ttgatggagt cacgccttgc
     9661 gcccggtgaa gcgcgactca gctacgccga ccgggtgctt tcgattctga aaagtgccgg
     9721 tttttcgacc caggcgcagt accgcgcatt ccagttgctc gacagctatg tttatggtta
     9781 cgtgctacaa gaggtcaatt ggccgacgaa agaatctgat cgcaagatgc tggttgttcg
     9841 gcttaaagaa tttttgcatg atggccggta cccgaatctt gtgagtgtga tcgagcaggc
     9901 aagctggctc aaggtcgcac ccggagaaaa gccttatgat gcagattttc aatttggtct
     9961 cgaactgatt ctcgatggta tcgaacgatt gttgtgattc aacagcaggt tcgcataata
    10021 agttgacaca ccggcaatag gcgacattgt cacgcagtgc cgcgctcacc gatcgatctc
    10081 gaaaacctcg acgaaaagca gctgatggag ctgcggctct gcgacctgcc gctcaagatt
    10141 gaaaacacct ggctcggcga gctcgtcgag cagctctaca gggaacttga atcacgggcg
    10201 ctggcctttc ggccgccgtg ttacctcggc gacgagtggt tttcacctga aggagttccg
    10261 gcgattgcgt tgccatttta ccttgcgcac ccgcggctca tacagatcga acgtaagctg
    10321 atcggtgagg cagaaggtga gagccgtgac gaagcgatgc gcctgctgcg ccacgagtgc
    10381 ggccatgcgc tggtgcatgc cttcaacctc acgaaacgcg cgagctggcg cgaggtattc
    10441 ggtcacccgc gcaaaccgtt cagcgattac taccgctacc agccttacag ccgctcgtac
    10501 gtgcgtaacc tgaaagactt ctacgcacag agccaccccg aagaagattt cgccgagact
    10561 tttgctgtct ggcttaaccc cgatatcaac tggcgcgaac tgtatgcggg ctggcccgcg
    10621 ctgagaaagc ttgaatatat cgaggagctc gtgcctgcca tcggcaataa gaccgtgagc
    10681 gtcgctaaat ccgaattctt ctggcatatt tcgacgctga agaagaagct cgattcacac
    10741 tacaaggcca agcgcaaact ctacatcgaa cacgaggcag attttttcga tgccgacctg
    10801 aagcagctgt ttcccgcgcc gcagggcaag tcgaagatcg cggcggcgcg ttttctcgcg
    10861 gggcagacga gcgatcttga acgcacggtc agtcgctgga cgggagaaaa gaaagttctc
    10921 ataaacaacc tgcttgcgcg gctgaagaag cgctgccagg tgctgaaact tgaagtgaat
    10981 gcgcgcggta ccgatgcggc gctcgtcgag tttaccgcgt acctcacgtc gctcgtgagt
    11041 aaccaccgct ataccaatcg ctataagcct ggaaaataac aatggcgagt gcagacaatt
    11101 ccgataatgc gcaggccgag cgcgcgaaag aacgccgtaa gcggcagaaa aaactcaaaa
    11161 tactcgtggc gttcgactac ccgctcgaac ctcccaaaga cggcaactac gccgcgcagc
    11221 tgcacgccga cgactggcgc ccgattcgta acgtgaccga ggccatcagc cgcctgggcc
    11281 acgaggtgat tttgcagggt gtctacgaca acatcatgcc gctgatctac acgatcaagc
    11341 gacgcaagcc cgatctcgcg ttcaacctgc tcgagagttt taagcagcag cgcaagtatg
    11401 aaggcgcgct cgcgggtctc tttgacctgc tgcaggtgcc gtacacgggt tgcccgtcag
    11461 aggcgctgac gatctgccgc aacaagcagt acacgaaaca gctgctgagc cagtacgaca
    11521 tccgcattcc gcgttcggtg ctttttccgc gcggcaagac gaaccgcagc gtgaaagatc
    11581 tcgcctttcc gctgattgtg aaaccgctcg ggctggaagg ctctgatggc atttcgcagg
    11641 cgtcattcgt cgaagatgaa aaggcgctca tcgagcgcgt gcgaaccctg catgaaacgt
    11701 acgaggtgga tgcgctggca gaggagtata ttgagggacg cgaaatctac gccggggtgc
    11761 tggggctcga taaactcacg gcgctacctc tgcgcgagat gctcttcacc aactttcccg
    11821 aagacaaacc taagtttgcg accttccacg caaagtggaa ccccgagttt cgcaagaaat
    11881 ggggcatcaa gaatactttc gccaaaggca tcgacgaaaa agtcgaagcg gaaattgaaa
    11941 acgtcagcaa gacagcgttt cgtgcgctcg gcctttcggg ttttgcgcgt ctcgatctgc
    12001 gcctgaccga taagaatgaa atatacgtga tcgaggtaaa tcccaacccc aacatcgcca
    12061 acgacgacga gattgcgtac gccgctcaac ggctgggcat ttcgtatgac cagctgatac
    12121 agcgcctgat cgattacggc cggcgcaagg tttagcggct ttcctgttgt atacccagca
    12181 ggctataggt gagttctatc cacaaaaaat ggccaaagtc cagaaatagg attatcatcc
    12241 tattggatat agggcactgc tccttggctg gtttttgaaa gttcgggcct gtagccgcaa
    12301 ttcacgcggt ttttcccgct gtatttattt gcgccggcca atccaaattt gattcaataa
    12361 gtgaggtgta atgaaagtac aacagagaat taaacatttc cacacgattc tttttctgct
    12421 cgcgctgtcg acggtggctg tggcctgcgg gtcgacagac aagaaagcag acagcaatgc
    12481 ggcaggggca actggcgcca gcggggccag ctcgcgctcg ttcggcggcg acatggttgt
    12541 cgatgcggcc aatgaccaat tggcaaaagt tcccgtggtg ggatttcccc ctttctccaa
    12601 cacgatgccg atgacacagt ttgaccagta tggcgagaat gcggcaaccg tagcgaaagg
    12661 tgtggtcaac aatatgcctg acggttatgt gctgcagatc aagggccatg caaaccagca
    12721 cccctctaag tcagctgcgt atacaaaatc gctctcgctg cagcgcgcga agtatgttta
    12781 caattatttt gccggtaaag gtatcgataa gaagaaaatg accgccgtgg gtgttggtaa
    12841 cgctgagcca gacccgagcc tttcgcacgc cgaaaaccgg cgcgtgactt tcaagctcgt
    12901 accaaagtaa tagtcttttt tgtcgcgtct cctggcctcg cgtaactgtc ttacgcgagg
    12961 ccttttttca cgaaagtgcc agctttttta ctgccggatg gagtgtcttt ttccaatggg
    13021 ctaagttgcc agatacctcg gcgttgacct gcgcataact ttcgggcgta ccacaatcat
    13081 accaaagccc ttcgtgcaca taggcacaga cctttttgcc tgcggcaatg agacccgtat
    13141 agccggtata gacaatgctc gatggttcgg atttcagaaa agcgaataac tccggttcga
    13201 acacggcaat acccgcgtag agatggttgc ctgtgctgtc tttgtcgaag gtgcggttaa
    13261 tatcagtgat atgtccctta tcgtcgatcg tgatgggcgc catggcattg gcctgcgggt
    13321 gcggcaccac aaccagcgtc agcgcggcct gttgcgcccg gtgtaaggtt atgagtttta
    13381 cagcatcgac gtcacagacc acgtcaccgt ttgcgagtaa taccgtgtca tcgtcgagta
    13441 aaccttcgca ctttttcact cccccgccgg tatcgagcag ctccgtttcg tgagacaggt
    13501 gcagctgcca tggcgtttcg gcgcgggcga agatctggtt tatggattcg gcgcaatggt
    13561 gcgtgttgac cgcgagcttt ttcacttttg cgtggtaaaa aaaactcagc gaataggcca
    13621 gcatcggcag gcccgcaaca ggaagagcgg gtttagctgt ctgctcagtc aggtgcttca
    13681 ggcgtgtacc gcgcccggct gcgagcagaa aaccattcat gttgaggccc attgctaaca
    13741 cgcagtggcg tggcaaccgt ttgaacctgt acctggtttt gtcctgcctg ccagggtata
    13801 ccctgatttt ctcagtatct ctaccgattg ccgtttatct cccgcgggag tatctttgca
    13861 gtgttcggat tgcctcgaat tccagaatgg actattgata caactgacca tgcagactga
    13921 tcccgcgacc attcttttgc tgacggcgat tgttgctatt gtaacaggtg ttatcctctg
    13981 ggcttttgag ctgacgcaga agcctggcat tccgcacctg cgcatgtggg catacgggac
    14041 attgctacag ggcaccgggc ttcttgttgt catgggtctg aggactgcgg ttccttacca
    14101 gcttggtatt cttagcggga acatgttgct catgtcggcc gtcttgcttt acggttcggt
    14161 tattgcagcg tatactgcgc gaactatccc gcgtagcttc tggtttctgc tgcttcttct
    14221 ttttgcagca tcgctttctg tgtaccttta cctctggcca aacctgcggg cgcgcgtggc
    14281 aatcttctca gtattcgctg cgttcgttct gtttgccagt gcactttggc tctattcttc
    14341 tgcttccaat aaaaatccca gtgagagatt tgttgctttc ctttttgctt tcactggcct
    14401 gggcatgacg ctgagggccg cagtccagta cccgtttgaa cccacaaccc aggacctgct
    14461 ttactccaac tctgcttatg ctggtgtatt ccatatgctt gcgctggtat cgaccctggt
    14521 gagcaccctg ggggttactt ccctctgtat tgagcggtcg cgttcagaaa gccaagaggc
    14581 tcaacgcgaa gctgccgctc agcgtgagat ctttcgccgc cagtttgaga tgcttcctgt
    14641 ggccgcatgc atctggagaa ttgagggtaa tgattacgta cttgagcagg caaattttgc
    14701 cgcgcaggtg ctgaccgagg gacgaatctg gacgcagacg ggtgggctgg ccagccagct
    14761 gctcgcccgg catcctgagg cgatcgaagg gttgaatcgc tgtcgcaacc agctcgacta
    14821 ctttgtc
//
LOCUS       DAOQJF010000031        14704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1958_length_14704_cov_5.143969, whole genome shotgun sequence.
ACCESSION   DAOQJF010000031 DAOQJF010000000
VERSION     DAOQJF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_14704_cov_5.143969"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..779)
                     /locus_tag="PLF85_02970"
     CDS             complement(126..779)
                     /locus_tag="PLF85_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNE18649.1"
                     /translation="MAKFRLISFKLCPFVQRSVIVLKEKKIPFEIDYIDLANKPGWFL
                     KLSPTGKVPVLEVDGKVLFESAVIMEYLDEVNPPSLHPKDAFERAQDRAWIEFSSGLL
                     VLQYQLSHTKSAEDYAAKLAALRAGLARFDSEFRGPWWHGDNFSLVDAAIAPLFTRIG
                     YTESNLPDKVLSGMPRMAAYGQSLEQREAIRDSVLPEVPEAYRASLRAAGGVIAQWL"
     gene            complement(826..1476)
                     /locus_tag="PLF85_02975"
     CDS             complement(826..1476)
                     /locus_tag="PLF85_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18650.1"
                     /translation="MKKVVITICFLCLGFIPVEACPITKAKNLKEIKLRDGTVKLGTL
                     QVSAKNQDPIVAFYREGKPVFCSADYDTSPVDARAVTATADKEHLYVVFSTDGGAKHP
                     KTFTRFTASGWQESYGAGGGAKVLVILKIRKADGVAIAGTYITAKKQDGKTNSVALKK
                     LEFNNNSVRLVADAWYSPLKTDKTPYECSGSSPFAYILQLSADLTTAMSTAVPQCK"
     gene            1601..1762
                     /locus_tag="PLF85_02980"
     CDS             1601..1762
                     /locus_tag="PLF85_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18651.1"
                     /translation="MIPLEWTVEYSTGIPKIDSQHAYLFELANRLTRAVAGGRSQEVL
                     SEIIRELND"
     gene            1781..2002
                     /locus_tag="PLF85_02985"
                     /pseudo
     CDS             1781..2002
                     /locus_tag="PLF85_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2046..3398
                     /locus_tag="PLF85_02990"
     CDS             2046..3398
                     /locus_tag="PLF85_02990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE18652.1"
                     /translation="MNFLSLAFVGFVTLTFVVYWNLPRRLRVGFLALASVFYYGAYSP
                     AFLAHFLAVIGLNFVIASALAKNRSRSLVALGVGLNLLHLGFFKYFNSLFVLLLPGAA
                     AESSFRVIMPLAISFYTFQFIAFQVDCYRGQVSKVEPLRFTLFMLFFPHLISGPILRS
                     GDLYSAIGNETESREMQTRGLYLIGLGLFKKLLIADVIGYLVNPVFKSPAEYGAIDTF
                     AAILGYTVQLYCDFSGFIDIARGISKLFGYDMPVNFRSPYFAASFSEFWTRWHITLSK
                     FIRDYIYIPLGGNRVSPARQYFNLMLAMVLSGLWHGETLNFLLWGALHGIYLAGEKLM
                     GLAKEPANRGTWLLRNLWVVGGWAFSLVFFRAADFPSALTLLRQLASVQPSSLNLALI
                     FALIVLAWLVQYAEARRETLLSLALPRAAYLLPLVLIVIYVIIIRIQIPNEAFIYVQF
                     "
     gene            3385..4473
                     /locus_tag="PLF85_02995"
     CDS             3385..4473
                     /locus_tag="PLF85_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNE18653.1"
                     /translation="MSSSDSRMKQKRYWLYYPVLLLVLLFVADKALLCRSHNGVTEGG
                     GIRLTETARIRLDKEASFVARKPGARTVVVFGSSRSERFNLLKQENQISEYLNPKQKE
                     QIQQTQFLVYAQVGAQMVAYYHALDHLWRRNMIPDAVVLELGPELLELEHKNNHGKSE
                     FIFADEYDYEYYRFLEKYGKKSVAEEARARLLFAGYAIKPRPELLVHDYRGKAQDEQP
                     EYSLIEKYVRSYKDYLDSDLTGKLREERVGQFVTMYGNNYKIFAVDPLMDESLRRIVK
                     MAKEKNVPLLLYKPFVHEELKAVLDKTPYAPAVDRYAKELVGGTVSFHYASQNDYQCK
                     YWSDASHYSTRCTPEIMYHLLSVLKFFP"
     gene            complement(4511..4852)
                     /gene="cutA"
                     /locus_tag="PLF85_03000"
     CDS             complement(4511..4852)
                     /gene="cutA"
                     /locus_tag="PLF85_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNE18654.1"
                     /translation="MKINPKNLPVIILTTLPDSVAAEKLSMLLVERKLAACVNIIPGI
                     RSIYIWKDKLVRDDEVKLLIKTSSARAPEAQKLIRENHSYSVPEITTLGLNGDVAMEP
                     DYWRWLTDYLQ"
     gene            complement(4963..5346)
                     /locus_tag="PLF85_03005"
     CDS             complement(4963..5346)
                     /locus_tag="PLF85_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18655.1"
                     /translation="MKKLILISALASLLVAAVSAGPIRDDYFKTRDDLEKSKDTPESR
                     KQFLETNIQRSLRRLLVRYYGYPTPEKVKISASNYEQSEKDKLTYYFKFEEYIGFLTF
                     SNDPEYYYAVPREEKILQKQKAEGK"
     gene            complement(5434..6060)
                     /gene="plsY"
                     /locus_tag="PLF85_03010"
     CDS             complement(5434..6060)
                     /gene="plsY"
                     /locus_tag="PLF85_03010"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNE18656.1"
                     /translation="MDSLQAIYVASLSYLAGSIPTAYIAGYLVKGVDIRTIGSGNVGA
                     SNAMRLLGKKWGIGILLFDAIKGLIPVLLVQHTFFAADAAVQRYAMVSALAAMVGHVF
                     PVWLGFSGGKGVATGLGVMTALVPVAVAVALPVFIVVVAISRYISLGSIISALVLPAA
                     FFIDHDLAGSAELFAFVCIACLFVIYKHKSNIKRIVAGEENRLGEKKT"
     gene            complement(6128..7219)
                     /locus_tag="PLF85_03015"
     CDS             complement(6128..7219)
                     /locus_tag="PLF85_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNE18657.1"
                     /translation="MEQQLRELASRAGEKLDRVEASAPALVAGRVYTQRKRTLVRPVS
                     VAPVILGCLILSGTIGISLSAPSGIKEDAIIEQVSPGNAETVWKREVTPATGPAYHLT
                     LDLRNRLTAQETLSQRVLWQQVLPPVAASSAPIVVGERKQLYVTVATSIGAVYHIAAN
                     SGQILWMQNLSDKVEVSPLPVKDRIITVACADGRIYGLNRTDGHIEYMIQTGSRIAAL
                     EPVADGIGEHIYAVADGRRVLALNASTGDLQWRRDTFGTVSDSPLVAAGTIITPTTEG
                     GDSKLWAFDQAGEIKWINTFDRYTNLTAAEDYIAMSQGSLVTLIRATTGEPVHYWQLA
                     ESPADISLAREGNRMVVRTDQGMLVSALN"
     gene            7417..7761
                     /locus_tag="PLF85_03020"
     CDS             7417..7761
                     /locus_tag="PLF85_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18658.1"
                     /translation="MTQSKGSLLALLLASVFAVGYCGSAKIPDDSPDIYPSSVEKADA
                     DAAIADAQAAYNALGDDGSAETGKAKEFLDKAKEFRNDNEPEAAWAAAIKAKSYCALS
                     KLLQQTRAAGLK"
     gene            7796..8509
                     /locus_tag="PLF85_03025"
     CDS             7796..8509
                     /locus_tag="PLF85_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18659.1"
                     /translation="MKKLIPVLLVAATLSPAFAGVIKESQAKREVDDAIQALAAMDSR
                     QDLATFLPQKEAYSARTYRNKASNFLSDSEYDKASFYAILSTNYSKISIAKGLLAKAE
                     HDKLAALAEGRAMEMVAPLLKSAGLKQKGSSGVFAGTYDLKALYGLKRTPKVDEVPAL
                     TDDAKKNMADISGVVAGQKDVKVTIVAKGKTEDHAAKYADSVKFAMVDKGVDAARINV
                     TTKKGKDGVEVTLDGVKTR"
     gene            complement(8520..10004)
                     /locus_tag="PLF85_03030"
     CDS             complement(8520..10004)
                     /locus_tag="PLF85_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE18660.1"
                     /translation="MAAVKSELHPLVELAGDEEIWAQIDLDKNSRRQVLSLIAEGIPA
                     LPFSKLSLQNSQTGNSVFRRKNIAGEAWLVPQRFERMVDFFLRRSEAVQPQADSAAIT
                     PLLASGANCLLTGGPGTGKSFQIAALVNHLAQQSTGRPLRVTIAAPTGKAAARFSHLK
                     PTGGVLLEWSTIHRLLGLSGDFSEPRFHSRHPLGIDLLIVDEISMLDLGLFAALIDAL
                     PDHARVVLAGDLGQLPAVDGMPIDHCLAFLENCKLVTRVHLTKVFRFTEARSLTYQRI
                     AQSGLSAIDEACEGVSLHRLKNAAECRAMLERNAAERFHSQTASDFRTRLVADAERGN
                     PDTALAREIFAWLKEQTVLTTRREGLMGSNLLNTAIAARVAQSSRDRALVPIIATNNN
                     YRLGIFNGDTGFITSVQGREYAVMEASEGEVICVPLSELSSWQGAYAITVHKSQGSEY
                     GEVWLVYEENEKAVFEDFRLLYTAVTRAKNHAHILQVTATGKRP"
     gene            complement(10015..12897)
                     /locus_tag="PLF85_03035"
     CDS             complement(10015..12897)
                     /locus_tag="PLF85_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNE18661.1"
                     /translation="MKHTGSITATEIVESGRHAFVEASAGSGKTTLLTSLFREIIASG
                     RAEIGQILCVTFTEKAASELKAKVYRELASQPDNELCRRAVARFAENQIGTIHSFCLR
                     SLALAPLARLAENTAEGDGDTEIFEEAREWVYRTCWAELDKPLLARLLAEAKFGSGGQ
                     QRQFDADLKQKALWCFAAGTTPIVPFIEEKHAVVDSASFKAWTLLLTVRKMHELAALR
                     NQMTFSRMITGLAEALVDEGFSQKIRDLFQYALIDEFQDTDAVQWNIFRTLFLSVDNH
                     ARLVVVGDPKQAIYKFRGADVFVYLAARRELERLGAVMGQLATNYRSNEELLAFQNWL
                     FSAAGPRSVWSSADIVYHEPVCGRAGEASTQQAVTTDAVVEICTAAEYRTEALKDFAA
                     ATVQRIAELRAANPAWTCAIIAYRHRSLRVFAEALRSAGIEFSYYKQQPDFGRTEVVH
                     LRSLLYSLTLDADQGLAVAESTLFLRGRNDAPAWYYRLHQLGRDGRIVSLLTWIAQDA
                     HLLHRVLECGGDTTHYYAWRTLVQTLLSACGRAVHDLDSLLHYLENLAAGDEDNPAAA
                     DILRDQSAVTLLTVQSSKGLDWNIVVVADGHNDNRWANFAFFHNREGEAIVPADTEDF
                     VARGDFLLEPEAEARITQLNLLYVALTRAKDRLLFLSAPSSRSQKPGPMAFFLGAVDA
                     SNVPAGTHVRELRPAEPVHEVRTTLPVAEPARVEHGNIPLRYGERTSFTALSEAHFTE
                     TEFLDDILPRGAETGQLLHNFLETTDLAALGASTPDYSAATLAHILAAVREQITDTEP
                     LIEPIANRIYSIVHATATSRLPLPNGNSVRLCDIPADNLWREMPFWSSPQIHRVLRQK
                     NDAVRRIMHGYMDLVFTPDGKGYFILDYKSNSLSGVDPALIGRYVNEHYGLQAQIYAE
                     ALDAYLQVHYRGTGRRVLGCYFVFLRYLAPDSTTGVHFIDVNTLKG"
     gene            complement(12894..>14704)
                     /locus_tag="PLF85_03040"
     CDS             complement(12894..>14704)
                     /locus_tag="PLF85_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18662.1"
                     /translation="DFAEAFHTFLQPLVTARTRLANRTGEKLADEMLALQHEISGAGE
                     SSQTLAAWFDQVRMLQGFAALTLPHIVKLLKRHLPGKSLAQQTGREGITFSSLAASCY
                     TRDTQLLFDLSEDADRQDKQVEYLFPEIQAAPTRLTGFEQLANQLATVLSSDSRYIIL
                     AYSAQDPASGTEKYPSQALAEVQGAAKILGRTEATRRNFSPSLLSVDATHPPIAADAD
                     RRTAWLLQRPSTHLQPLSAFTLPNLTNDMSMAATDLRDLLAYLKNPARHILRRHLPPD
                     LAIAEFNRDEPKLAVGNDARLRFCEEYLELVLLDAGDRTPPAASNFIRFKQARGDYAP
                     EGFDQAGRLLAEGDNDRRLSGLAHKLRDDYRLVEYIFHSGVTKPFAVEETARLTRRYH
                     PAIRLLETSITGSSGILLQDPEGRLFWLQSAIYGERNTSLIELHLLLCIFALAEIQLS
                     NPAITLADFATNTRSKDPIGAIDFTPRTGLPLPDASSARAYLERLLTDITAQHIVWYD
                     HSLVKDPKLAEWSDMPDDEILQRLAKQPEKNTNEDLQRLQSYFALEADSGSVSFFETF
                     IRPVAQLDRRENPAKTPAGAAGPAKGKTGKKKAGKT"
BASE COUNT         3218 a         3966 c         4148 g         3372 t
ORIGIN      
        1 tgaaagcagc agggcagaga acgccaccag tgcggccagg cgggcagtac gggtgttttg
       61 gaccatgatc cattatcgac atggtataca cccgatctga aagtcagcgg cgctttggcg
      121 gctgctcaca gccactgtgc gataacgcca cctgccgcac gcaggctagc ccggtacgcc
      181 tcgggcactt cgggaagaac cgagtccctg atggcctcgc gctgttcgag gctttgcccg
      241 tatgctgcca tccggggcat acctgacagt actttgtccg gcagattgct ttcggtatag
      301 cctatgcggg tgaacagcgg ggcaatcgcg gcgtcgacga ggctgaagtt atctccgtgc
      361 caccaggggc cccgaaattc gctgtcaaac cgggcaaggc cagcacgtag cgctgccagt
      421 ttggccgcat aatcctcggc agatttcgta tgcgaaagct ggtactgcag aaccagcagg
      481 cccgatgaaa attctatcca ggcgcgatcc tgcgcccgtt cgaacgcatc tttcggatgc
      541 aatgacggtg ggttaacctc gtcgaggtat tccatgatca ctgcagattc aaagagcacc
      601 ttgccatcga cttcgagtac cggcactttg ccggtcggcg aaagtttcag gaaccacccg
      661 ggtttattgg cgagatcgat ataatctatt tcaaagggga ttttcttttc tttcaggaca
      721 atcaccgagc gctgcacaaa aggacagagc ttgaagctga tgagacggaa ttttgccatt
      781 tgcctgtagc ttaatgcccg gcgtgcccgg tggcgagaaa acttctcact tgcattgtgg
      841 tacagctgtc gacattgcag ttgtcaggtc ggctgaaagc tgcagtatat aggcaaacgg
      901 cgaagagcca gagcattcgt aaggagtttt atctgtttta agtggcgaat accaggcgtc
      961 ggcgacgagc ctgaccgagt tgttgttaaa ctccagtttc ttcagcgcaa ccgaattcgt
     1021 tttgccatcc tgcttttttg ccgtgatata agtgccggcg atcgcgacgc cgtctgcctt
     1081 acggatcttg agaatcacca ggactttcgc tccgccgcct gcgccatagg actcttgcca
     1141 gccagaggcc gtgaagcgcg taaaggtctt ggggtgtttc gccccgccat cggtagagaa
     1201 aacgacatag aggtgctctt tgtctgctgt ggcggttact gcgcgcgcat cgactggcga
     1261 ggtgtcatag tcggcagagc agaaaacagg ctttccttcg cggtagaagg cgactatggg
     1321 gtcctggttt tttgcgctta cctgcagtgt tcctagcttc accgtgccat cgcggagctt
     1381 aatctctttg agatttttag ccttcgtgat cgggcaggct tcaaccggga taaaaccgag
     1441 acacagaaaa caaattgtta taacgacttt tttcacaggt caaagcaata tggaagccac
     1501 ggttacgcaa gtcgaattcg tatttcttta ccgcaatttg ccgcgcggca gtattgtctc
     1561 ggcttgacgg catgaaaaac ctgcaggacg gtctttgcac atgattcctc tcgaatggac
     1621 ggtcgaatac agcacgggaa tacccaaaat tgactcgcag catgcgtacc tgtttgagct
     1681 ggccaaccgc ctgacgcgcg cagtggcagg cggccgcagc caggaagtcc tttcagagat
     1741 cattcgtgag ctcaacgact aagtcgcgag ccactttagc tacgaagagt cggtgatgga
     1801 acaggcgcac tacgaaggtc ttgctgtgca ccggcagatg cacgaaaaaa tgcgcgaaga
     1861 gcttacccgc tacgctacag aactcgcggc gggtacgctg aatgctgcaa tgctcagcaa
     1921 gtttctcgaa cgctggctga ccggtcatat tatgcacgaa gatatggcat acattcccgc
     1981 ggtggccgca gcgcagcaat aatacttgct tgcccgaaat ggcttcttaa gctggcgaag
     2041 acgccatgaa ctttctatcg ctcgccttcg tcgggtttgt gaccctgact tttgttgttt
     2101 actggaacct gccgcgccgg ctgcgggtgg gctttctggc gctcgcttct gtcttctact
     2161 atggtgcgta tagcccggct tttctcgcgc actttcttgc cgttatcggc ctcaatttcg
     2221 tcatcgcttc ggctttggcg aagaaccgga gccgcagcct ggttgctctc ggtgtgggcc
     2281 tgaacctgct gcatctcggc ttcttcaagt acttcaactc gttgtttgtg ctgttattgc
     2341 caggtgctgc agccgagtca tcgttcaggg ttatcatgcc attggcgatc agcttctaca
     2401 cgttccagtt tattgcattc caggtcgatt gctaccgggg gcaggtgagc aaggtagagc
     2461 cgctgcgttt tacgctcttc atgctttttt tcccgcatct gatttcgggg ccgatactgc
     2521 gttcaggtga cctttattcc gcgataggta atgaaaccga atcgcgcgag atgcagaccc
     2581 ggggtttgta cctcatcggc ctggggctgt tcaaaaaact gctgatcgcc gatgtcattg
     2641 gctacctggt caacccggta tttaagtcac ccgcagagta cggcgcaatc gacaccttcg
     2701 ctgcaatact cggttatacc gtgcagctct actgcgattt ttcgggcttc atcgacattg
     2761 cgcgggggat ttcaaaatta ttcggctatg acatgccggt caattttcgc agcccgtatt
     2821 ttgccgcaag tttctccgag ttctggaccc ggtggcatat aacgctctct aagtttatcc
     2881 gtgattacat ctacatcccg ctgggcggca accgcgtatc acccgcgcgg cagtacttta
     2941 acctgatgct cgcgatggtg ctgtcaggtc tctggcatgg tgaaacgctg aattttttgc
     3001 tttggggtgc gctgcatggt atttaccttg caggtgaaaa gctcatgggg cttgcgaaag
     3061 aacccgccaa ccggggcacg tggctgctgc gcaacctatg ggtggtcggc ggctgggcgt
     3121 tctcgctcgt cttctttcgc gccgcagatt ttccgtcggc gctgacgttg ctgcggcaac
     3181 ttgcctcggt tcaaccttct agtctaaatc tcgcgctgat ttttgcgttg attgtgctgg
     3241 catggctggt gcaatatgcc gaggcgcgcc gcgaaacgct actctctctc gcgttgccgc
     3301 gtgcggcgta cctgctcccc ctcgtgctca tcgtcatcta tgtaatcatt attcgtatcc
     3361 agattcccaa tgaggccttc atctatgtcc agttctgatt cgcgcatgaa acaaaaacgc
     3421 tattggctct attatcctgt gttgctgctg gttctgctct ttgttgcaga caaagcgttg
     3481 ttgtgccgca gccacaatgg ggttaccgag ggtggcggta tacggctgac cgaaacggcg
     3541 cgcattcgcc tcgacaaaga agcgagcttc gtcgcgcgta agccgggtgc gcgcacggta
     3601 gtggtttttg gcagctcgcg ttctgagcgt ttcaacctgc tcaaacagga aaaccagata
     3661 tctgaatatc tcaatccaaa acagaaagaa cagattcaac agacacagtt cctggtctat
     3721 gcgcaggtcg gagcgcagat ggttgcgtac taccatgcac tggatcacct ctggcgcagg
     3781 aacatgatac ccgatgcggt tgtgctcgaa ctggggcctg aactgctcga actcgagcac
     3841 aagaacaacc acggcaagtc cgagttcatt tttgccgacg agtacgacta cgaatactac
     3901 cggtttctgg aaaagtatgg caagaagtca gtggcggaag aagcccgcgc acggttgctg
     3961 tttgccggtt acgccatcaa accccgtcct gagcttctgg tacacgatta ccggggtaag
     4021 gcgcaggacg agcagcccga atattcgttg atcgaaaagt acgtgcgcag ttataaagat
     4081 tacctcgaca gcgatcttac gggcaaactg cgcgaagaaa gggttggcca gtttgttacc
     4141 atgtatggca acaattacaa aattttcgcg gtcgatccgc tcatggatga atcgctgcgg
     4201 cgtatcgtca aaatggcaaa agaaaaaaat gtgcccctgc tgctatacaa accttttgta
     4261 cacgaggagc tcaaggccgt actcgacaaa actccgtacg caccggcggt tgaccgttat
     4321 gcaaaagagt tggtcggcgg cacggtcagt tttcactacg cgagccagaa tgattaccag
     4381 tgcaaatact ggtcagacgc cagccactat tcgacgcgct gcactcctga gatcatgtac
     4441 cacctgctga gtgtcctgaa gtttttcccc tgaacggatg tccgtttcag agagatgatt
     4501 tatcgcctcg ctactgcagg tagtccgtaa gccagcgcca gtaatcaggt tccatcgcga
     4561 cgtcgccgtt taggcccagt gtcgtaattt ccggcacact ataggagtgg ttttcgcgga
     4621 tgagcttttg tgcttccggc gcgcgggcgc tcgaggtctt gatcagtagt tttacctcgt
     4681 catcgcgcac gagcttgtcc ttccagatat agatggagcg gatgccaggg ataatattca
     4741 cacaggcggc aagtttgcgc tcgacgagta acatagatag tttttctgcc gcaacactat
     4801 cgggtaaggt tgtgagtatg atgacaggca aatttttagg atttattttc ataataccgg
     4861 agatagttca ggcgaaaaca ataagtggtt cgtcagaggg aaaaatcccc cgtgaccggc
     4921 cagtgtgccc ttgaccggtc aggagggcga acctgagtca ggctatttgc cttctgcttt
     4981 ttgcttttgc agaatttttt cttcgcgggg aaccgcgtag taatattccg gatcattcga
     5041 gaaggtcaga aagccaatgt actcttcaaa cttaaaatag tacgtcagct tgtctttctc
     5101 tgactgctcg tagtttgaag cgctgatttt tactttttcg ggagtagggt agccgtaata
     5161 acggaccagc aggcggcgca gtgaacgctg gatattggtt tccaggaact gcttgcgcga
     5221 ttcaggggta tctttgcttt tctcgaggtc atcacgcgtc ttgaaatagt cgtcgcgtat
     5281 cggcccggca gatactgcag ccaccaacag ggaggcaagc gcggaaatca ggatcagttt
     5341 cttcataagt tactcctatg gtacacgaag tacctgcata acggtcgata ctcttttctt
     5401 atcagccgtc acctttcacc agcgggacaa aaatcatgtc ttcttttcac cgagacggtt
     5461 ttcctcacca gcgacgatac gcttgatatt ggacttatgc ttgtaaatca caaaaagaca
     5521 ggcgatgcag acgaaagcga agagttctgc gctgcccgcc aggtcgtgat cgataaagaa
     5581 ggctgcgggc aggacaagag cagaaattat cgagccgagc gaaatgtaac gcgagatggc
     5641 aacgacgacg atgaagacgg gcaacgcaac tgccacggca acaggtacca gggcagtcat
     5701 gactcccaac cctgtggcga cgcccttacc cccgctgaaa cccaaccaca caggaaacac
     5761 atggccgacc atagcggcta aggctgaaac catcgcatat ctctgtacgg cagcatcggc
     5821 cgcaaaaaag gtatgctgca ccagcaggac ggggattaat cccttgatcg cgtcaaacag
     5881 cagtatgccg atgccccatt tttttcccag aagtcgcatc gcattcgaag ctcccacatt
     5941 gcccgagccg atggtacgga tgtcgacgcc cttgaccaag tagccagcaa tataggccgt
     6001 cggtattgag cccgcgaggt atgaaagcga ggcgacataa attgcctgga gggaatccac
     6061 agatgcgact acgctttaag tgcagcgcgt caagaaatct agcggggtgc cggaatcggg
     6121 tgacccgtta gttgagcgct gaaacgagca tgccctggtc ggtgcgcaca accatgcggt
     6181 taccctcgcg cgccagtgaa atatcggcag gcgattcggc gagctgccaa tagtgcaccg
     6241 gttcgcccgt cgttgccctg atcagagtca cgagactgcc ctgcgacatg gcgatatagt
     6301 cttcggccgc ggtaaggttc gtatagcgat cgaacgtgtt gatccatttg atctcacctg
     6361 cctgatcgaa tgcccagagc ttgctgtcgc cgccctcggt tgtgggagta atgatagtgc
     6421 cggctgcgac cagtgggctg tcgctgacag tcccgaaggt gtcgcggcgc cactgcaggt
     6481 cgcctgtgct tgcgttcaac gcgaggacac gcctgccgtc ggcaaccgcg tagatgtgtt
     6541 cgccgatacc atctgccact ggttcgagcg ctgcgatacg cgagcctgtc tggatcatgt
     6601 attcaatatg gccgtcggtt cggttcagtc cgtagatgcg cccatcggcg caggcgactg
     6661 taatgattcg gtctttcacc ggcagcggtg acacttcgac cttatcgctg agattctgca
     6721 tccacagaat ctggccggaa ttcgcggcaa tgtggtaaac cgcaccgatc gatgtagcca
     6781 cggtaacata gagttgcttt ctttcgccga ccacgatggg ggcagagctc gcagctacag
     6841 gcggcagtac ctgctgccag agcacacgct gcgaaagtgt ttcttgcgca gtcaatcggt
     6901 tccgcaagtc gagcgtcaag tgataggctg gccccgttgc aggagttacc tcgcgtttcc
     6961 agactgtctc ggcattaccg ggactcacct gttcgatgat ggcgtcctct ttaatgccgg
     7021 aaggtgccga aagtgagatg ccaattgtgc ccgagaggat caggcaaccg agaatcaccg
     7081 gagctacaga taccggacgg accagggtac gcttgcgctg cgtgtatacg cggccggcaa
     7141 caagtgccgg tgcagatgcc tccacgcggt ctaatttctc acccgcccgt gaggccagct
     7201 ctctgagttg ctgctccata cactaactta cggtaaaata gccttgcgtc cggaaaaaat
     7261 tgccagtagc cccggtcaga aattggcttt taccttgacg cacaattcct ctgctctgcg
     7321 atgggtcaag gactgggaac tttacccggt tttgcgaacc caaaggcctg tgatcgggtc
     7381 tgtaggtata taataaccaa taaacaggag ttttggatga cacaaagcaa aggttcacta
     7441 ttggctctgc tgctcgcgtc ggtatttgcc gtcggctatt gcggtagcgc aaaaatcccg
     7501 gatgattctc cggatatcta tccttcgagc gtagaaaaag ctgacgctga tgcggcaatt
     7561 gccgatgcac aggccgcgta caacgcgctg ggcgacgacg gcagcgccga gaccggcaaa
     7621 gcgaaagaat ttctcgataa ggcgaaagaa ttccgcaacg acaatgaacc cgaagcagcc
     7681 tgggctgccg cgataaaggc aaagtcgtac tgcgcacttt caaaactgct gcagcagaca
     7741 cgcgcagcgg ggctcaaata agcggcactg cccgtcttaa attaaggaga caaacatgaa
     7801 gaaactcata ccagtactgc tcgtagctgc gacgctttca cccgcattcg caggagtgat
     7861 caaagaatca caggcaaaac gcgaagtaga cgatgcaatc caggcacttg ccgcgatgga
     7921 ttcccgtcag gatctcgcaa ccttcctgcc gcagaaagag gcatactcag cgcgtactta
     7981 ccgcaacaag gcgagcaatt ttctcagcga cagcgagtac gacaaggcca gcttctatgc
     8041 gattctttcg accaactatt caaagatcag catcgccaag ggcctgctcg caaaagctga
     8101 acacgacaag ctcgcagcac ttgcagaagg ccgcgcgatg gaaatggtgg ccccgctgct
     8161 caaatccgca ggtctgaaac agaaaggttc aagcggcgtg tttgccggta cttatgacct
     8221 gaaggccctt tatggtctga agcgtacgcc gaaagtagac gaagttcctg cgctgaccga
     8281 cgatgctaag aagaacatgg ctgacatttc tggcgtcgtt gccggacaga aagacgtcaa
     8341 agtcaccatc gtggcaaaag gtaagaccga agaccacgcg gcgaaatacg ctgacagcgt
     8401 aaaattcgcg atggttgaca aaggcgttga tgcggcacgc atcaatgtca ctaccaagaa
     8461 gggcaaagac ggcgtcgaag taaccctcga cggcgtaaaa acccgctaac cggaaactgc
     8521 tacgggcgct tgcccgtagc agtcacctgc agaatatgcg cgtggttttt cgcgcgcgta
     8581 actgcggtgt acaacaggcg aaaatcttca aataccgctt tttcattctc ttcgtaaacc
     8641 agccaaactt caccatattc agaaccctgg ctcttgtgca cggtgattgc atatgcgcct
     8701 tgccagctac tcagctctga caggggcaca cagatcactt ctccttcgct cgcttccatt
     8761 actgcgtatt cgcggccctg cacacttgtg atgaatccag tatcgccgtt gaagataccc
     8821 aaccggtagt tgttgttagt ggcaatgatt ggtactaatg cccggtcacg ggaactctgg
     8881 gcaacgcggg ctgcgatggc cgtgttgagc aggttgctgc ccatgagtcc ttcacgtcgc
     8941 gtggtcagca cagtctgctc tttcagccag gcgaagatct cgcgggccag ggccgtatcc
     9001 ggattccctc tctctgcatc cgcgaccagg cgcgttcgga aatcgctggc agtctgcgaa
     9061 tgaaaacgtt ctgcagcatt gcgctcgagc attgcccggc actccgcggc atttttcagg
     9121 cggtgcagcg aaactccctc gcaggcctcg tcaatcgccg acaaacccga ttgggcgatg
     9181 cgctggtagg taagtgaccg cgcctcagtg aagcgaaaaa cctttgtcag gtgtactcgt
     9241 gtgacgagtt tacagttctc gaggaatgcc aggcagtggt cgatcggcat gccgtcgaca
     9301 gcgggtagct gtcccaggtc gcctgctagc acgacccggg catggtcggg tagtgcgtcg
     9361 ataagcgcag cgaaaaggcc tagatcgagc atcgaaattt catcgacgat cagcaagtca
     9421 atgcccagag gatgccggga atggaaacgc ggctcagaga agtcgccact gaggccgagc
     9481 aggcggtgga ttgtcgacca ttcgagcagg actcctcccg tgggtttcaa gtgcgagaaa
     9541 cgcgctgctg ccttgcccgt cggggcggca atggtaacgc gcaacggccg ccccgtggac
     9601 tgctgcgcca ggtgattgac gagcgctgcg atctggaaac tcttccctgt gccaggtccg
     9661 cccgtgagca ggcagttggc ccccgaagcc agcagtgggg ttatcgcggc gctgtcagcc
     9721 tgtggctgta cggcctctga ccgtctcaaa aagaaatcca ccatgcgctc gaaacgctgt
     9781 ggtacgagcc aggcttcacc cgcgatattt ttcctgcgga atacagaatt accggtttgc
     9841 gaattttgca gcgagagctt gctgaagggc agtgcaggaa ttccttcggc aatgagactg
     9901 agcacctgcc tgcgggaatt tttatcgaga tcgatctgcg cccagatttc ttcgtcgcca
     9961 gccagttcga cgagggggtg gagttcactt tttactgcag ccatgattcg atcttcagcc
    10021 cttcaatgta ttcacatcga tgaaatgtac gcccgttgtg gaatcgggcg cgaggtaacg
    10081 caggaaaaca aaataacagc caaggacgcg gcgtcccgtg ccgcggtagt ggacctgcag
    10141 gtatgcatcg agtgcttcgg catagatctg cgcctgaagc ccatagtgct cattgacata
    10201 cctgccgatg agtgccgggt caactcctga aagagaatta ctcttgtaat cgagtatgaa
    10261 gtagcccttg ccatcgggtg tgaagacgag atccatatag ccgtgcatga tgcgccgcac
    10321 ggcatcattt ttctgccgga gtacacggtg aatctgcggc gagctccaga aaggcatctc
    10381 gcgccagaga ttatcggcag gaatatcgca caggcgaacc gaatttccgt tgggtagcgg
    10441 aagcctcgat gttgcggtgg catgcacaat gctgtagatt cggtttgcaa tgggttcgat
    10501 cagtggttct gtatccgtaa tctgttcacg caccgcagcg aggatgtgcg ccagcgttgc
    10561 ggccgaataa tcgggagtac ttgcccccag agcggcaagg tcggtggttt cgaggaagtt
    10621 gtgcagaagt tgtcctgttt ctgcgccgcg tggcaggata tcgtcgagaa attccgtttc
    10681 ggtaaaatgg gcttctgaga gagcggtgaa tgaagtgcgt tcgccatagc gcagcggtat
    10741 attaccatgc tcaacgcgcg ctggttcagc tacgggcaga gtcgtcctga cttcgtgcac
    10801 aggttcagca ggccgcagtt cgcggacatg ggtgccagct ggcacattgc tggcatcgac
    10861 agcgccgaga aaaaaagcca tgggtcccgg tttttggcta cgcgagctgg gtgcagaaag
    10921 aaacagcagc cggtctttcg cacgggtcag tgccacgtaa agcaggttga gctgcgtgat
    10981 gcgcgcctcg gcctccggtt cgagcaggaa atccccccgg gccacaaaat cttccgtgtc
    11041 ggcgggtact attgcttcgc cttcgcggtt atggaaaaaa gcgaaattcg cccagcggtt
    11101 atcgttgtgg ccgtcagcca cgaccacaat attccagtcg agtccttttg aactctgcac
    11161 ggtcagcagg gtaacagccg attggtcgcg caggatatcg gctgcggccg ggttgtcttc
    11221 atccccggct gcgagattct ccaggtaatg cagcagcgaa tcaagatcgt gcaccgcgcg
    11281 gccacaggcc gaaagtaagg tctgcaccag cgtacgccat gcatagtagt gggtggtatc
    11341 tcccccgcat tcgagcacac ggtgcagcag gtgtgcatcc tgtgcgatcc aggtgagcag
    11401 gctgacgatg cgaccatccc tgccgagctg gtgcagccgg tagtaccagg caggtgcatc
    11461 gtttctgccc ctgaggaaaa gcgtcgattc tgcgacggcg agaccctggt ctgcatccag
    11521 ggtcagggaa tacaacaagc tccgcaggtg cacaacttca gtacgcccaa aatccggctg
    11581 ttgcttgtaa taagaaaact cgataccagc actgcgcagc gcttcggcaa aaacccttag
    11641 cgaacggtgt cggtacgcaa tgatggcgca cgtccatgcc ggattcgccg cgcgcagttc
    11701 tgcaatccgt tgaacagttg cagcggcaaa gtctttcagc gcttctgtcc tgtattcggc
    11761 cgcggtacag atctccacca ctgcatcggt ggtcacagcc tgctgggtac ttgcttctcc
    11821 ggcgcggccg cataccggtt cgtggtaaac gatgtcggca gagctccaaa ccgaccgggg
    11881 ccccgctgcg ctgaacaacc agttctggaa cgcgagtaat tcttcgttgc tgcggtagtt
    11941 ggtggcaagc tgccccatga cagcgcccag ccgttccagt tcacggcgtg cggccaggta
    12001 gacaaagaca tcggcgccgc gaaacttgta aattgcctgc ttgggatcac caacgacaac
    12061 aagccgcgca tgattatcga ccgaaagaaa cagcgtgcgg aaaatattcc actggacggc
    12121 atcggtatcc tggaactcgt caatcagtgc gtactggaat agatcgcgaa ttttttggga
    12181 aaaaccttca tcgactaatg cttcggccag ccctgtgatc atgcgcgaga aagtcatctg
    12241 gttgcgcagg gccgccaatt catgcatctt gcggactgtc aaaagcaggg tccacgcttt
    12301 gaaagatgct gagtcgacga cggcgtgttt ctcttcaata aatggcacga tgggtgttgt
    12361 accagcagca aaacaccaca gcgctttttg tttcaggtct gcgtcaaact ggcgttgctg
    12421 gccacccgag ccaaatttgg cttctgccaa tagcctggcg agcaggggtt tatccagttc
    12481 tgcccagcag gtgcggtaga cccattcgcg ggcctcttca aaaatttcag tgtcaccgtc
    12541 gccttcggcg gtgttttcgg cgaggcgtgc gagcggcgcc agcgccaggc tgcgcaggca
    12601 aaagctgtgg attgtaccga tctggttctc ggcgaaccgc gccaccgcgc ggcggcagag
    12661 ttcgttatct ggctggctgg cgagttcgcg gtataccttt gctttgagtt cagaagcagc
    12721 tttttccgtg aatgttacgc agagtatttg cccgatttct gcacgcccgc ttgctataat
    12781 ctcacgaaag agcgaggtga gcagcgtggt cttgcctgaa ccagcagatg cttccacgaa
    12841 ggcgtgcctg cccgattcga caatttccgt ggccgtgatg ctgcccgtat gcttcacgtt
    12901 ttgcccgcct tcttcttccc ggtttttcct ttcgcggggc ctgcagcacc ggccggcgtc
    12961 ttcgcaggat tttccctgcg gtcgagctgt gctaccgggc gaataaaagt ttcaaaaaag
    13021 ctgacgctcc cactgtcggc ttcgagcgcg aaatagctct gcagccgctg caggtcttcg
    13081 ttcgtatttt tttccggctg ctttgccagt ctctgcagga tttcatcgtc cggcatatcg
    13141 gaccattcgg caagcttggg gtctttgacc agcgagtggt cgtaccacac aatatgctgc
    13201 gccgtgatat ctgtgagcag gcgttccagg tacgctcttg ccgagctggc atcgggaagc
    13261 ggaagacccg tacgcggcgt aaagtcgatg gcgccaatgg gatctttact acgcgtgttc
    13321 gtggcaaaat ccgccagcgt aattgccggg ttgctcaatt ggatttcagc cagtgcaaag
    13381 atgcacaaca acaggtggag ctcaatcagg ctggtattgc gctcaccgta gattgcagac
    13441 tgtagccaaa acaaccgacc ttcaggatct tgcagcagga tgccgctgct gcctgttatg
    13501 ctggtttcga gcaggcgtat cgcagggtga taccgccgtg tcagccttgc agtctcttcc
    13561 acggcaaagg gtttggtgac cccactatgg aaaatgtatt ccacaagcct gtagtcgtca
    13621 cgcagcttgt gagccaaccc ggaaagccgg cgatcgttat caccttcggc gagcagcctg
    13681 cctgcctggt cgaaaccctc aggtgcgtaa tcgccgcgtg cctgtttgaa gcggataaaa
    13741 ttgctggcgg caggcggcgt cctgtcaccc gcatcgagca ggactagctc caggtattct
    13801 tcgcagaagc gcaggcgggc atcgttgccc accgcgagtt tcggttcatc gcggttaaat
    13861 tccgcgatag ctaaatctgg tggtaggtgg cgacgcaata tgtgacgggc agggttcttc
    13921 aggtaggcta gcaggtcgcg caggtcggtc gcggccatcg acatatcgtt agtgaggttt
    13981 ggcagcgtga atgccgaaag tggctgtaga tgagtggagg gcctctgcag caaccacgcc
    14041 gtcctgcgat cggcatcggc agcgatgggt ggatgagtgg catcgacaga caacaggctc
    14101 ggcgaaaaat tccgcctggt ggcctctgtg cggcccagta ttttggcagc accttgcact
    14161 tctgccagtg cctgcgatgg atatttttct gtgccgctcg ccgggtcctg tgcgctgtac
    14221 gcgaggatga tataccggct gtcgctgctg agtactgtcg ccagctggtt tgccagctgt
    14281 tcaaaaccgg ttaaacgcgt cggggcagcc tgaatttccg ggaacaggta ctcaacctgc
    14341 ttgtcctggc gatcggcatc ttcactcaga tcgaacagta gttgtgtatc gcgcgtgtag
    14401 cacgaagcgg cgagtgacga aaaagtaatg ccttcgcgac cggtttgctg ggccagactt
    14461 ttgcccggca ggtggcgctt tagcagcttc acgatgtgcg gcagggttag ggccgcaaac
    14521 ccctgcagca tcctgacctg gtcgaaccat gccgcgaggg tctgtgaact ttcgccagcg
    14581 ccggaaatct cgtgttgcag cgcgagcatt tcgtctgcaa gcttttcgcc ggtgcggttt
    14641 gccagcctgg ttcgcgccgt tacgagtggt tgcaggaatg tatggaatgc ttcggcaaag
    14701 tccc
//
LOCUS       DAOQJF010000032        14687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_1962_length_14687_cov_5.529114, whole genome shotgun sequence.
ACCESSION   DAOQJF010000032 DAOQJF010000000
VERSION     DAOQJF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14687
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1962_length_14687_cov_5.529114"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PLF85_03045"
     CDS             complement(<1..1016)
                     /locus_tag="PLF85_03045"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNE18663.1"
                     /translation="MKSRTLNKEELESFGAELDQLRAETLAKVGEEDAQYIREIVQLC
                     RDYEILGRELLKTEGQNPISWLKGAYSLGIAKILENMEIGHNVMHGQYDFMNDPDLHS
                     QTYEWDTASHSDGWRHSHNYMHHTYTNIVGKDRDLGYGILRLTDDEPWKPQHLLQPIS
                     NTALSLLFEYGVALHDLETDRIKDGEKQLALKDVVPVLRKIFAQAAKDYVYFPATAGL
                     NAVPVLTGNIAANLMRNVWAHAIIFCGHFTSDAETFKQEEVTDESRGAWYLRQLRGSS
                     NLTGDWWFHILSGNLSHQIEHHLFPDIPAWRYAEMAPRVREICAKYGQHYNTGSFVKQ
                     YGEVL"
     gene            complement(1094..2185)
                     /locus_tag="PLF85_03050"
     CDS             complement(1094..2185)
                     /locus_tag="PLF85_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE18664.1"
                     /translation="MENTVHTHLLDAFQRFERLLPNYARTGIFSPSAQQYIVGRSGNY
                     RPAAQVIARRQETADTISLVIQTPADFPTFRAGQYIDVAVQIRGVRHVRQYSITSPQG
                     DSALRITIKRQSGGLVSGYLHEKATEGTTVEISQPKGDFVLPQKPFGSYVFCSAGSGI
                     TPVFAMVQKLLAENTQSPIHLLHAARSEEAIIFRAELEHLAEKHANFFPHFFLSEASD
                     FQKRRLNAVGAFSLLGTEHETAASAVFLCGPGEFVRDLETAFRDRGYSHIATEYYTLP
                     ATTSTEEGTVQFVRSGIEVKASGNLLETAEAAGLKPKHGCRRGICHECKAHKKTGSVK
                     NILTGQLTQGEENIQLCVSQAIGRVEVEM"
     gene            2812..3099
                     /locus_tag="PLF85_03055"
     CDS             2812..3099
                     /locus_tag="PLF85_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNE18665.1"
                     /translation="MDNNSKSSRQLRIEKLLRVAFPVAAFEVFDDSKEHAGHAHGQSG
                     ETHYRLRITDESFRGESRLQSQRRILAALKPEFDDGLHSFVIEEIASAPRP"
     gene            3096..3500
                     /locus_tag="PLF85_03060"
     CDS             3096..3500
                     /locus_tag="PLF85_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE18666.1"
                     /translation="MTTTTSSNDHLSLTFAALADPTRRAMLVRLGQGPATVSELAQPF
                     QISAPAISKHIKVLEKAGLITREKEAQWRRCRIEAQRLREANEFIERYRKFWEDSFDR
                     LELYLQKLQAKDTLPEKSAKAGAAGSIEGEGI"
     gene            3512..4159
                     /locus_tag="PLF85_03065"
     CDS             3512..4159
                     /locus_tag="PLF85_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE18667.1"
                     /translation="MGKVKVAAFAVSLDGYGAGPGQSLENPLGERGTELHNWFFPTRT
                     FQSMHAGSDQGTTGIDNTFAERSFENVGAWILGRNMFGPVRGPWINDDWKGWWGENPP
                     YHVPIFVLTHHKREPLRMAGNNTFYFVTDGIESALAQAKAAAGNKDVRIGGGVSTVRQ
                     YLQKGAIDEMHLAFSPVLLGRGENLLHGLDLPALGFTKIEHTMGEGALHLLAKKE"
     gene            4163..4579
                     /locus_tag="PLF85_03070"
     CDS             4163..4579
                     /locus_tag="PLF85_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE18668.1"
                     /translation="MDKTKITIEAQVAAPVKKVWDYYNQPQHIIRWNFAHESWQCPSA
                     ENDLRPGGKLKSRMEARDGSFGFDFEATYDEVADLKRIVYTIADGRRVETDFTDVEGN
                     TRIVTVFEAESQNPVEMQKGGWQAILDNFKKYTEAN"
     gene            4579..5199
                     /locus_tag="PLF85_03075"
     CDS             4579..5199
                     /locus_tag="PLF85_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE18669.1"
                     /translation="MATKKSKAKPAKKGAKAKSEGSATGLRYVGAKAHQITPFLMFNA
                     NPEEAAKFYVSIFKKKSKILSSNPMQADFVLNGQRFFSYNGGDHPNFKFNWGVSFMIS
                     VETQKEVDYYWKALTADGGEESMCGWVKDKFGLSWQVTPNILLKLISHKDKTIADRAQ
                     QAMLKMKKIDIAALKAAINLTPQPPLRRRRPETGHLKERGSALARG"
     gene            5215..5745
                     /locus_tag="PLF85_03080"
     CDS             5215..5745
                     /locus_tag="PLF85_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE18670.1"
                     /translation="MENINYRELLNREVVLTRVIAAEKKMVFEAFSTAEALGEWFGPA
                     GFTTTTFEFDFKVGGRWRFEFKGPDGTIYGNRIEYAEISPFDRIAFHHGSDVDDDPGR
                     FFVTITLDEQQNKKTVVSLRQLHPTAERRAAVIGFGAVEFGMQTLDKLAEFVAKLPHP
                     SSPASGGGAHQGGGGL"
     gene            5742..6215
                     /locus_tag="PLF85_03085"
     CDS             5742..6215
                     /locus_tag="PLF85_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE18671.1"
                     /translation="MKNFELKLERVLDAPRNLVWRCWTEPQHLMPWFCPEPWKVVDCR
                     IDLRVGGEFFNVMQSPEGERFPNDGVYLEVVPQTRLVWTNAYKAGWYPNPGANPMLTT
                     IVLEFEDLGNGKTNYRGMARHWTPEATEQHEKMGFYEGWGIAADQLVAYAKSMKA"
     gene            6217..7167
                     /locus_tag="PLF85_03090"
     CDS             6217..7167
                     /locus_tag="PLF85_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE18672.1"
                     /translation="MEPVKSITITRIFDAPRQLVFEAWIDEKHLQQWWMPRGFTNPTC
                     RITASSRPGYAETGAELTVVTYHPAFGDMPQTGKFEEVSPFDKIIFSTTAFPNAEGKP
                     QLRGRNVIVFSDEPAQGKAKTRMVITASILEAGPGLEGALAGMDQGWAETLDRLSEHV
                     EDTSEREMLITRVVDAPRDLVWKIWTEPEHVKEWWGPNGFTNTIHKFDLVPGGEWSLT
                     MHGPDGADYPNQIRFIEIIRSERLVYDHGQPEDGGSFRTFVHFSDEGGKTRVTMKGVF
                     KTAEYRQLLIDKYGALEGQQQHIARMEDYLKQFKNGEVRK"
     gene            7164..7523
                     /locus_tag="PLF85_03095"
     CDS             7164..7523
                     /locus_tag="PLF85_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HNE18673.1"
                     /translation="MSYIDGFVIPVPLAKKEAYFEMARMAKAIYIECGAIRVVEAWND
                     DLQKGKINDFRTAVIAEEDEGVVFSWIEWPDKATRDAGNQKIMNDPRMATPDGMPFSG
                     ARLIFGGFSIFSDTDKQ"
     gene            7533..7937
                     /locus_tag="PLF85_03100"
     CDS             7533..7937
                     /locus_tag="PLF85_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE18674.1"
                     /translation="MKNQTPSKAAFRTGWALSGLAIAFLTFDGVLKFFLHKMPPEAMA
                     DSAKLAYPMNIMPGIGATLLICVLLYAIPRTAVLGAILLTGYLGGAVSAHVRIMNPWP
                     SHILFPVYFGVFIWLGLYLRVPALRNFLPLKK"
     gene            7947..8801
                     /locus_tag="PLF85_03105"
     CDS             7947..8801
                     /locus_tag="PLF85_03105"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE18675.1"
                     /translation="MPLKKFCSLFSLSVLVSACSGASQPDVKGLATVNGIQMYYETYG
                     AKQDIPLLLIHGGASTIESTFGKIISLLAKNHYLIAIEEQAHGRTADRNAPLRFKTSA
                     EDAIALLEHLKIGKADVFGFSNGASIAMRMAIQHPNKIRKLIFASSLTRRSGAPAQLW
                     DFIAKSDISQMPEPLKQAFLKVNGDPEKLKNLHAKTRERMVNFQDTPNEEVRLIQAET
                     LILTGDRDVAILEHSLELVRTIPKARLMILPFGHGDYIGEASSLPADSRIPDFTVGFI
                     EDFLHKPF"
     gene            8811..9236
                     /locus_tag="PLF85_03110"
     CDS             8811..9236
                     /locus_tag="PLF85_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002118579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE18676.1"
                     /translation="MRLNSIYLNFAGNTEEVFLFYKSILKREFDSLVRFKDMPMPGVT
                     LSESDSNKIMNIAIPIGNHSALMGSDALESLGQKVVQGNNFYINLSPESKPEADRLFA
                     GLSAGGSVEMAMADMPWGDYWGCFRDKFGIGWMISIPSK"
     gene            9262..9738
                     /locus_tag="PLF85_03115"
     CDS             9262..9738
                     /locus_tag="PLF85_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE18677.1"
                     /translation="MKTTESVTFVVSRLGCGDIASFRELNLIFSEVFEDTANYQSRPP
                     SDNYLNGLLAKQHIIALVARVDGRIEGGLVAYVLDKFEQERSEVYIYDLAVREKFRRR
                     GIARNLIFELRKIAHEAGAWVIYVQADKAEEDLPAQKLYESLGKREDVFHYDIAVN"
     gene            9738..10355
                     /locus_tag="PLF85_03120"
     CDS             9738..10355
                     /locus_tag="PLF85_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE18678.1"
                     /translation="MSKLAAAEKIYFADREALRTWFLKNHAKVESFWLVYDKVKEGKR
                     HLTYDDIVEESLCFGFIDSQPRKVSDTQASCYISRRKPKSEWSKRNKEKTAELIRTKL
                     MHASGFAAIARAKENGSWSKIDGSENHEMPEDFAKILHKQKNLRQHFESLAPSMRKAF
                     LHRLQSARTPETRQKRLDFLVQLLKVKIRPSEYRAAFTKGVVPKR"
     gene            10648..11037
                     /locus_tag="PLF85_03125"
     CDS             10648..11037
                     /locus_tag="PLF85_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE18679.1"
                     /translation="MAMSKAKLITYWVTTVLLAANYAFAGVMYIGKGEPVVEGAKALG
                     YPLYFMVLLGYWKLAGAAAILAPRTPLIKEWAYAGMFINLTAASISNYVSGLGAKEII
                     APLTMIPFLAISWALRPESRRFNGPLL"
     gene            11050..11502
                     /locus_tag="PLF85_03130"
     CDS             11050..11502
                     /locus_tag="PLF85_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE18680.1"
                     /translation="MSAADSELIIERVVNAPRELVFKVWTTPENMLEWWGPTFCPSKQ
                     VDMDVRPGGKWRCCLDSQIHGGDLWQNGVFREVRPYDLLEFTFVWEAEGDMGVENVVT
                     IRFFDEAGKTRMHFRQAPFTAVSQRDANNGGWSNMFIRLEDYLQQVKQ"
     gene            11682..14273
                     /locus_tag="PLF85_03135"
     CDS             11682..14273
                     /locus_tag="PLF85_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE18681.1"
                     /translation="MNTNTEQKERMIDRWLNKYTRFMTAIPEFSQRRRWSILGFFAAL
                     TFALSFGLGNLKVDQTFDTWLDAGDSSLLDYHRFRYFFGSDEYVLIMFKPAGKEVFDA
                     DTLKKVKALEDDLNKKRIEKDSPLGRITRIRTIVSADFLESKNDTLKNRKFIGQTIPD
                     NKLLAEIKKVAFAQRDFPGSFFSKNSEFGAIMILTDYGARIQKSKDPQKAAGSAEEFD
                     FSDSKAAKKDRNKVPKMESADPTDYLAFNAELEKVLKAHNWANPTVGEPSSQVMDYLI
                     GGGPWVASFFMNIMMTQMGMIMGLALLLILVTLAIAFRSLSAMLWPTLIIALSVVWTL
                     GLIGLADVTMTMMINIVVILNLTVGISSSVHIISGYKYFNVTLDKRPALTKAYEKSGI
                     SIMLATLTTLAGMLSLIVVPIVPIRNFAVAASCGILFAYVGTIFLLPALLGIWAPGAS
                     RDKTTGEKMQGLFERQIQRFIGFIMNATHDRPKLIIGVFSAIMVLSLTGYPRVIVDTN
                     FLESVKPGYGFKETFETLDQHFGGTTNVEIVINTGKNDGLKDVQLLTAMDQLAERAKK
                     ERPDFVTKTTSLVNAVKDAHKNLMSRGPNKPGTESPYYSIPADNQKLKQVLLLYDSAD
                     PESRKLFADDDWEKGRITLQVVNKSSFEYVEFLKQVESWLKEYLEPLKKSNVELSYYA
                     TGGIPIMMRLTGFLTESQISSFGLALVVITIIMFLIYGTIRYGMIAMLPNIFPILLTV
                     GVTGWAKIPLDSDTLLVLPIAIGIAVDDTIHFLTHYRGELHAGHNRLEAIKTTLREVG
                     QAMIFTSVILSVGFLIFLPSVYVPFRNFGMLSAIAISSALFADLFFLPALLTVFGKSN
                     KVKGAHS"
     gene            14270..>14687
                     /locus_tag="PLF85_03140"
     CDS             14270..>14687
                     /locus_tag="PLF85_03140"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNE18682.1"
                     /translation="MNQRNLLQNITRARSARVIFCLLFSAPLAAQNLKEPEKAKQIAY
                     KMIKRDDGKTSYNRTKVITCAFEEQGGKRKCSGQARTKSFESISKDVGPQLEDTIGLS
                     VIDDPADEKGSAFLQKDYEVPGKDTEQWMYLPALRKL"
BASE COUNT         3536 a         3717 c         4019 g         3415 t
ORIGIN      
        1 agcacttcac cgtactgctt cacgaaacta cccgtgttat agtgctggcc gtacttcgcg
       61 cagatttcgc gtaccctggg cgccatttca gcatagcgcc acgcgggaat gtcggggaaa
      121 aggtggtgtt cgatctggtg gctcaggttg cccgacagaa tgtggaacca ccagtcgccc
      181 gtcaggttac ttgaaccgcg caactggcgc aggtaccatg caccgcggct ttcgtcggtg
      241 acttcttcct gcttgaatgt ctctgcatct gaagtgaagt ggccgcagaa gatgatcgcg
      301 tgtgcccaga cgttgcgcat caggtttgcc gcaatatttc ccgttagcac cggtacggca
      361 ttcaaaccgg cagtcgcggg aaaatagacg tagtcttttg ctgcttgagc gaaaattttt
      421 ctcagcaccg gcacaacgtc tttcagcgcg agttgttttt cgccgtcttt gatgcgatcg
      481 gtttcgaggt cgtgcagcgc tacgccgtat tcgaaaagca gcgagagcgc ggtgtttgaa
      541 atgggttgca gcaggtgctg tggtttccac ggctcgtcgt ctgtcaggcg cagaatgccg
      601 taacccaggt cgcggtcctt gccaacaatg ttggtgtacg tatggtgcat gtagttgtgc
      661 gaatggcgcc agccgtctga gtgagaagcg gtatcccatt cgtacgtctg cgagtgcagg
      721 tcggggtcgt tcatgaagtc gtactggccg tgcatcacgt tgtgcccgat ttccatgttt
      781 tcaagaattt tcgcaatacc gagtgagtat gcacccttga gccaagaaat cggattttgc
      841 ccttcggtct tgagcagttc gcgacccaaa atttcgtagt cgcggcagag ctgcacaatt
      901 tcgcgaatat actgagcatc ttcttcaccg accttagcca gggtttctgc gcgcagctgg
      961 tcgagctcgg cgccgaatga ttcgagttct tctttgttga gtgttctgct tttcatggtt
     1021 ttctccttat accggtcact tcgactccgc tcagtgaccg agtgagctcg gtgagcggag
     1081 tcgaaccgag tgattacatc tctacttcta ctcttcctat tgcctggctg acacagagct
     1141 ggatgttctc ttcgccctgc gtcaactggc ccgtcagaat attcttaacg ctgccggttt
     1201 ttttgtgcgc cttgcactcg tggcagatgc cgcgccggca gccgtgcttg ggcttgagac
     1261 ccgctgcttc tgcggtttcg agcaggttgc cgctcgcttt cacttcaatg ccagaacgta
     1321 caaattgcac cgtaccttct tcggtgctgg tggttgcggg cagggtgtag tattcggttg
     1381 caatgtggga gtatccccgg tcacgaaacg cagtttcgag atcgcggaca aattctcccg
     1441 ggccgcagag gaaaacggca gaagccgcag tctcgtgttc tgttcccaga agagaaaaag
     1501 ctcccactgc attcagccgc cgtttctgga agtcactcgc ttcgcttaaa aagaaatggg
     1561 gaaaaaagtt cgcgtgtttt tcggcaaggt gctcgagctc tgcgcgaaag atgatcgcct
     1621 cttcagaccg cgcggcatgc aggaggtgta tgggtgattg tgtgttttct gccagtagtt
     1681 tctgcaccat tgcgaatacc ggcgtgatac cgctgccggc agagcagaaa acataagagc
     1741 caaacggctt ttgcggcagt acgaaatcac ccttcggttg tgaaatctcg accgtcgtac
     1801 cttctgtggc cttttcatgc aggtagcctg aaacgaggcc accgctctgc cgcttgatag
     1861 tgatgcgcag ggcgctatcg ccctgcggtg aggtaatgga atactgccgc acatggcgca
     1921 cgcctcgaat ctgcacggca acatcgatgt actgaccggc gcgaaatgtc ggaaaatcgg
     1981 ccggcgtctg aattaccagg ctgatggtgt ctgcggtctc ttgccgccgc gcgatgacct
     2041 gtgccgcggg acggtagtta ccactgcggc caacgatata ctgctgcgcc gaaggactga
     2101 agatgcctgt gcgcgcatag ttgggtagca gccgctcaaa gcgctggaat gcgtcgagta
     2161 ggtgtgtgtg gacggtgttc tccataaagc ctcctggtgt tgttgcctgt ggcagcgtcg
     2221 ctggagacaa tctaacggtg ttagatataa tgtaaacaat tgtttatctt tttctgcatc
     2281 cttccctttt tgtgcaagtg ccggctggga ggggctactg gtaagcgcag cggaagccaa
     2341 cgcgggggtt ggtataagtg cttgagggct cgaaatccag gtggtagact cctgattgtc
     2401 tgccgccttt gagaccggta aaccgctgca tccggcgccc gtagggtcat tactgcattt
     2461 catctcgtat ttcgcgacgc agaaatttgc agtcgtgccg acagcgttgt tgaaaggcac
     2521 gggtatgtaa ttcgtcgggc aatttgcatt gctgatgatc gtgatgagcg atgcgaggct
     2581 tgtgccgctc gcatcgctca ccgttacgac tgctgctgaa atagatttca tttcccctca
     2641 ttcaccaaca gggctatccg gacccgggtt actgaaaacg taaacaagaa atgccgaaaa
     2701 gctcggcgtg agcgtatggg aagattatac aatacggctt gacgctttgc cttgttttta
     2761 acttattggt taattaacta ataagttaaa tacaggagcg ggtataggat tgtggacaat
     2821 aattcaaaat caagccgcca gctgcggata gagaaattac tcagggtggc gtttcccgtt
     2881 gcagcctttg aggtcttcga cgattcaaaa gagcacgccg gtcacgcgca tggccagagc
     2941 ggcgaaacgc actaccgtct gcgaatcacg gacgagagtt ttcgtggaga atcaaggctg
     3001 cagagccaga gacgcattct cgcagcgctc aagcccgagt ttgatgacgg cttgcattcg
     3061 tttgtcatag aagaaatagc ttctgcgcca cggccatgac aaccacgacg agcagcaatg
     3121 accacctgag cctcacgttt gccgcgctcg ccgacccaac tcgacgtgcg atgctggtgc
     3181 gccttggcca gggacccgca acggtgagtg agctcgcgca accgtttcaa atcagtgcac
     3241 cggcaatctc gaagcatatc aaggtgctcg aaaaggcggg gctgatcacg cgcgaaaagg
     3301 aagcgcagtg gcgccgttgc cgcattgagg cgcagcgcct gcgtgaggca aatgaattta
     3361 tcgagcgcta tcgcaaattc tgggaagaca gttttgaccg cctcgagctc tatctgcaga
     3421 agctgcaggc aaaggataca ctgccagaaa agagtgcgaa agccggtgcg gcgggcagta
     3481 ttgaaggtga aggtatttag ggggagttag aatgggtaaa gttaaggttg cagcatttgc
     3541 ggtttctctc gatggctatg gtgccgggcc aggtcagagc cttgaaaatc ccctcgggga
     3601 acgcggcacc gagttgcaca attggttttt tcccacgcgt acatttcagt cgatgcacgc
     3661 gggttcagac cagggaacaa cgggtataga taataccttt gctgagcggt cttttgagaa
     3721 cgtcggcgcg tggattctcg ggcgaaacat gtttggaccg gttcggggac catggatcaa
     3781 tgatgactgg aaaggctggt ggggagaaaa tcctccttat catgtgccaa tctttgtgct
     3841 gacacaccac aagcgcgaac cgctgcgcat ggccggaaat aatacttttt attttgtcac
     3901 cgacggtatc gagtccgcgc ttgcgcaggc caaagctgcc gcgggcaata aagatgtgcg
     3961 cattggcggc ggtgtttcta cggtcaggca atacctgcag aaaggcgcta tcgacgagat
     4021 gcatctggca ttttcgccgg tgcttttggg caggggagag aacctgctgc acgggcttga
     4081 tctcccggcg ctcggtttta caaaaattga acataccatg ggcgaaggtg cattgcatct
     4141 gcttgcgaag aaggagtaaa aaatggataa aacaaaaatc acgattgagg cgcaggtcgc
     4201 tgctccggta aaaaaagtct gggattacta caatcaacct cagcatatta tccgatggaa
     4261 ttttgcgcac gaatcgtggc aatgcccgag cgccgagaat gacctgcgtc caggtggtaa
     4321 gctgaaatcg cgcatggagg ccagggacgg cagcttcggt tttgattttg aagcgaccta
     4381 cgacgaagtc gccgatctaa agcgaatcgt ctatacgatt gccgatggcc gcagggttga
     4441 aaccgacttc accgatgtcg aaggcaacac ccgaattgtg accgtattcg aggcagagtc
     4501 acaaaaccct gtggaaatgc aaaagggcgg ctggcaggcg attctggata atttcaaaaa
     4561 atacactgag gcaaactaat ggcaacgaaa aaatcgaagg caaagcccgc aaaaaaaggg
     4621 gctaaagcca aatctgaagg tagtgcgaca ggcctgcgtt atgtgggcgc aaaggcacac
     4681 cagattacac cctttttgat gttcaatgcg aatcctgaag aggcggcgaa attttatgtt
     4741 tcaatcttca aaaagaaatc gaagattctg agttcgaacc ctatgcaggc agattttgtt
     4801 ctcaatggac aaagattctt ttcgtataac ggcggcgatc accccaattt caaatttaac
     4861 tggggtgtgt cattcatgat cagcgtcgaa acgcagaaag aagtagacta ttattggaaa
     4921 gccctgactg ccgacggcgg cgaagagagt atgtgcggat gggtgaaaga taagtttggt
     4981 ctctcatggc aggtaacgcc gaatatattg ctcaaactca tttcgcataa agacaaaacc
     5041 atcgccgacc gcgcacagca ggcgatgctg aagatgaaga agattgatat cgcggctctg
     5101 aaggcggcga ttaacctcac cccccaaccc cctctccgaa gacggagacc cgaaacgggt
     5161 cacctcaagg agagggggag cgctttggcg cgggggtgag gcaaacggga gagtatggaa
     5221 aacattaact atcgtgaact tctgaatcgc gaagttgtcc tgacgcgcgt cattgcggcc
     5281 gagaagaaaa tggtctttga ggctttctca acagccgaag cactcggcga atggttcggc
     5341 cccgctgggt tcactaccac cacgtttgaa ttcgacttca aggtcggcgg ccgctggcgt
     5401 tttgaattta aggggcccga tggcaccatc tacggcaacc gcattgaata tgcggaaata
     5461 tcaccattcg accgcatagc ctttcaccac ggctctgacg tcgatgacga ccccggccgg
     5521 ttctttgtca ccattacgct cgatgagcag caaaacaaga agactgtagt ctcgctgcgg
     5581 cagttgcacc caacggccga gcggcgggct gcggtgattg gtttcggcgc ggtcgaattc
     5641 ggcatgcaga cactcgacaa actggcggaa tttgttgcga agctccccca cccatcctcc
     5701 cccgcaagcg ggggaggtgc ccatcagggc ggagggggac tatgaagaat ttcgaactaa
     5761 aattagaacg cgtactcgat gcgccgagaa acctcgtctg gcgttgctgg actgagccgc
     5821 agcacctgat gccctggttt tgtccagagc catggaaggt tgtcgactgt cgcattgacc
     5881 ttagggtggg cggtgagttc ttcaatgtga tgcaatcacc cgaaggcgag aggttcccta
     5941 atgatggtgt gtatctggaa gtcgtacccc agacgcggct cgtctggacc aacgcctaca
     6001 aggcaggttg gtatccgaat cccggagcaa atccgatgct cacaaccata gtgctggagt
     6061 tcgaagacct gggcaatggc aagacgaact accgcggcat ggcgcggcac tggacgcctg
     6121 aagcgacaga gcaacacgaa aaaatgggat tttacgaagg ctgggggatt gctgctgacc
     6181 agctcgttgc ttatgccaag agtatgaagg cttgaattgg aacccgttaa gtcgattact
     6241 attacccgca tattcgatgc tccgcggcag ctcgtcttcg aagcctggat agacgaaaaa
     6301 catctacagc aatggtggat gccgcgcggg ttcacgaacc ccacatgccg catcactgca
     6361 agcagcagac cgggatacgc tgagaccggc gccgagctga cggtggtcac ctaccacccg
     6421 gcttttggcg atatgccgca gaccggcaag ttcgaagagg tttcaccgtt cgataagatt
     6481 atcttttcca caactgcctt ccctaatgca gagggaaagc cgcaactcag gggtcgtaac
     6541 gtcatcgtgt tcagcgacga gcctgcccag ggcaaggcga agacccgcat ggtaattaca
     6601 gccagcattc ttgaggctgg tcccggtctt gaaggcgcgc tcgccggtat ggaccaggga
     6661 tgggctgaaa ctctcgaccg gctcagtgag cacgtcgagg acacatcaga gcgcgaaatg
     6721 ctgatcacgc gcgtggtaga cgcgccacgc gatcttgtct ggaaaatatg gaccgagccc
     6781 gagcacgtca aagagtggtg ggggccgaac ggatttacca atacgatcca caagttcgat
     6841 cttgtgcccg gcggtgaatg gtcgctgacg atgcatggcc ccgatggcgc cgactatccc
     6901 aaccagatcc gttttatcga aataatccgt tctgagcggc tggtctatga ccatggccaa
     6961 cccgaagacg gcggttcatt tcgtaccttt gtccatttta gcgacgaagg cggcaagacg
     7021 cgcgtgacca tgaaaggcgt ctttaagaca gcggaatacc gccagctgct tatcgataaa
     7081 tacggcgcac tcgaaggcca gcagcagcat atcgcgcgta tggaagatta tctcaaacaa
     7141 ttcaagaatg gtgaggtcag gaaatgagtt atattgatgg atttgttatt ccggtgccat
     7201 tggcaaaaaa agaggcctat ttcgaaatgg cgagaatggc caaggcgatc tatatcgaat
     7261 gcggcgcaat acgcgtcgtt gaagcctgga atgatgacct gcagaagggc aagatcaatg
     7321 attttcgcac agcagtaatt gccgaagaag atgaaggcgt ggtgttttca tggatagaat
     7381 ggcccgacaa agccacgcgt gatgcaggta accagaagat catgaatgat ccgcgcatgg
     7441 cgacccccga tggtatgccc ttcagcggcg cccggctcat cttcggcgga ttttctatct
     7501 tttccgatac agacaaacaa taagagacta tcatgaaaaa ccaaacacct tctaaagcag
     7561 cctttcgtac cggctgggcg ttgagcggcc ttgcaattgc ctttctgacg ttcgacggcg
     7621 tgctgaaatt ttttctgcac aaaatgccac ccgaggcaat ggccgactct gcgaagctcg
     7681 cgtacccgat gaatatcatg ccgggcattg gtgcaacgct cctgatctgc gtactgctgt
     7741 atgcgattcc gcgcaccgcg gttttgggag cgatcttgtt aaccgggtat ttgggcgggg
     7801 cggtttcggc gcatgtgcgc attatgaatc cgtggccgag tcatatactt tttcccgttt
     7861 actttggtgt ttttatctgg ctggggctgt atttgcgcgt accagcgttg cgcaattttc
     7921 tcccgctgaa aaaataaggc ctggcaatgc cgttgaagaa gttttgctct ttattcagcc
     7981 tctctgttct cgtttctgca tgctctggcg ccagtcagcc agatgtgaag ggtttggcaa
     8041 ctgtcaatgg tattcaaatg tactatgaaa cttacggagc aaagcaggat atccccctgc
     8101 tcttgattca tggcggtgcg tcgaccatcg aatcgacctt cggtaagatc atttcgctgc
     8161 tggcgaaaaa ccattatctc attgcgatag aagaacaggc gcatggtcgc accgccgatc
     8221 gcaacgcgcc tttgcggttc aagacatcgg ctgaggacgc aatcgcgctg ctggaacatc
     8281 tcaagatcgg gaaagcagat gtgtttggct tcagtaacgg tgcgagcatt gccatgcgta
     8341 tggctattca gcatcccaac aaaatccgca agctcatctt cgcttcatct ctaacccggc
     8401 ggtctggtgc gccggcgcag ttatgggatt tcatcgcgaa atctgatata tcacagatgc
     8461 cagaaccact caagcaagct tttctgaaag ttaacggcga tccggaaaaa ctaaaaaatc
     8521 tgcacgcgaa gacacgcgag cgtatggtaa actttcaaga tacgccgaat gaagaggtca
     8581 ggttgattca ggcagagacg ttaattttaa cgggcgatcg tgacgtcgct atcctggagc
     8641 atagccttga gctggtacgt accatcccga aagcgaggct gatgatttta ccttttggac
     8701 acggcgacta tatcggtgaa gcttcgtcgt tgccggcaga ttcgcgcatt ccagatttta
     8761 ccgtcggttt tatcgaagat ttcttgcata aaccatttta aggaggaatt atgagactca
     8821 acagcatcta cctgaacttc gccggtaata cggaggaggt tttccttttc tacaaatcta
     8881 tcctcaagcg cgagttcgat tcgctcgtgc gattcaaaga tatgccgatg ccaggggtaa
     8941 ctctttctga atctgatagt aataagatta tgaatattgc catcccgatt ggtaaccata
     9001 gcgcactgat gggctcggat gcgctcgaat cgctggggca aaaagtggtg caggggaaca
     9061 acttttacat caacctctca cccgaatcaa aacccgaggc ggatcgtctt ttcgccgggc
     9121 tttctgccgg cggttcggtc gagatggcaa tggctgacat gccgtggggc gattactggg
     9181 gctgctttag agataaattc ggcatcggct ggatgatcag tattccgagt aagtaatttt
     9241 tgccagcggc gactaaggta tatgaaaact acagagtctg tcacctttgt tgtctcccgt
     9301 ctcggatgtg gggacattgc ctccttccgc gaactaaacc tgattttttc ggaggttttt
     9361 gaggatacag caaactacca gagtcggccg ccgtcagaca actatctcaa tgggcttttg
     9421 gcgaagcagc acattatcgc cctggttgcc agggtcgatg gaagaatcga aggtggcctc
     9481 gtcgcctacg tgctcgataa attcgaacag gaacgcagcg aagtctatat ctacgacctc
     9541 gcggtacggg aaaaattccg ccgacgtggt atagcgcgca acctgatttt tgagctgaga
     9601 aaaattgcac acgaagcagg ggcctgggtc atctatgtac aggcagataa ggcagaagaa
     9661 gacctgcctg cgcagaagct ctatgagtca ttgggcaagc gtgaagacgt tttccattac
     9721 gatattgcgg tgaactgatg tcaaaactcg ctgccgcaga aaagatatac ttcgccgatc
     9781 gcgaggcatt acgcacctgg tttttaaaga accacgccaa agtcgaatca ttctggctcg
     9841 tttatgataa agtcaaagag ggcaaacgcc acctgaccta cgacgacatt gtcgaagaaa
     9901 gtctctgctt cggcttcatc gacagccagc cgcgcaaggt ctccgataca caggcaagtt
     9961 gttatatcag caggcgtaag cccaagagcg agtggtcaaa gcgcaacaaa gaaaaaaccg
    10021 cggaactgat aaggacgaag ctgatgcacg cgtcgggctt cgccgccatc gcacgcgcca
    10081 aagagaatgg ttcctggtcg aagattgacg gttcagagaa ccatgagatg cccgaagact
    10141 tcgccaagat actgcacaag cagaaaaacc tgcggcaaca tttcgaaagc cttgctccct
    10201 ctatgcgcaa agccttcctt caccgcctgc aatcggcccg gacaccagaa acccgccaga
    10261 agcggctgga tttcctggtg caattgctca aggtaaaaat tcgcccgtct gaataccgcg
    10321 ccgccttcac gaagggagtc gtaccgaagc gatgaaaaag acctgcccca acggccacac
    10381 ctttcagaag tcgagtgact gcccgacctg ccccatttgt gagcgcgcaa aatcacccaa
    10441 tccctcctct aaaaatggcc ttgaaaaaac gcggacattt gctgagaaaa aaatgacgtc
    10501 agcatccttt tttcctagat ttctgaacgg ttctcatagc gattgttttc cggtcccgtc
    10561 gggcaggctt tcgaccaatg ccccggctac caggtctcta cctgatgcag aacccgattt
    10621 tccaaaaaat aactcaccat tgaggagata gctatgagca aagcaaaatt gattacctac
    10681 tgggttacta cagtcctgct ggcggcaaat tacgccttcg caggggtaat gtacatcggt
    10741 aagggtgagc cggttgtcga aggggccaag gcgcttgggt atccgctgta tttcatggtg
    10801 ctgctgggtt actggaagct cgcgggtgcg gcagccattc ttgcaccgag aactccgctc
    10861 atcaaagaat gggcctacgc gggcatgttc attaacctga ctgcggcctc aatttcgaac
    10921 tacgtttcag ggctgggagc aaaagagata atcgcaccgc tcacaatgat tccgttcctg
    10981 gcgatttcct gggcactgcg ccctgaaagt cggcgcttta acgggcctct gctctgatag
    11041 tccggaaaaa tgtcggcagc agattcagag ctaatcattg aacgtgtcgt caacgctccg
    11101 cgtgagctgg ttttcaaggt ctggacaacg cctgagaaca tgctcgaatg gtggggtcca
    11161 accttctgcc cttcgaagca ggttgacatg gatgttcggc ccggcggcaa atggcgctgc
    11221 tgtctcgatt cacagattca cggcggcgac ctgtggcaga acggggtgtt ccgtgaagtc
    11281 agaccctatg atctgctcga gttcacgttt gtgtgggagg ccgagggtga catgggtgtt
    11341 gagaatgtcg tcaccatacg gttcttcgat gaagctggca agacgcgcat gcactttcgg
    11401 caagcgccct tcactgcggt ttctcagcgc gatgcaaaca atggcggatg gtcgaacatg
    11461 ttcatccggc tggaagacta tctgcagcag gtgaaacagt aaatgcaacg aggtccactg
    11521 gctcagccag aaaatccagt gagcacgacc gccaggtcgt gcgttacgat gcatgccccg
    11581 gttagtataa accggggcaa gatttttact ttacaggctt acggtgtaag ctattttaat
    11641 tgggctgctt gggctctaag gtccatccag cagggggagt tatgaacacc aataccgaac
    11701 agaaagagcg aatgattgat cgctggctga acaaatatac caggtttatg accgcgatac
    11761 ccgagttctc ccagcgccgc cgctggagca ttcttggctt ctttgcggcc cttactttcg
    11821 cgctcagctt tgggctcggc aatctgaaag tcgaccagac ctttgatacc tggctcgatg
    11881 ccggtgactc ttcgctgctc gactaccatc gttttcgcta cttctttggc tcggacgaat
    11941 atgtgctcat catgtttaag cccgccggta aagaggtctt tgatgccgat acgctgaaga
    12001 aagttaaggc tctcgaagac gatctcaaca aaaagcgtat cgagaaagat tcaccccttg
    12061 ggcgcataac ccgcattcgc acgattgtta gcgctgactt tctcgagtca aagaatgaca
    12121 cactgaagaa ccgtaagttt atcgggcaga cgattccgga caataagctg ttggcagaaa
    12181 tcaagaaagt cgcattcgcg cagcgtgatt ttcctggcag tttcttctcg aagaattctg
    12241 agttcggggc gatcatgatc ctgaccgact atggtgcccg catccagaaa agcaaagatc
    12301 cgcagaaggc cgctggctcc gcagaagagt tcgacttttc tgacagcaag gcggcgaaaa
    12361 aagaccgcaa caaagtgcca aaaatggaga gtgccgaccc cacggactat ctggcgttca
    12421 acgccgaact cgaaaaggta ttgaaggccc acaactgggc aaacccgact gtaggcgaac
    12481 cctcgtcgca ggttatggat tacctgattg gcggcggccc ctgggtggcg tcattcttca
    12541 tgaacatcat gatgacgcaa atgggaatga tcatggggct tgcattgctc ctcatactcg
    12601 taactctcgc gatcgcgttt cgttcgctct cagcaatgct ctggccgacg ctgatcattg
    12661 ctctgtcggt tgtctggact ctcggcctta tcgggctcgc tgatgtgacc atgaccatga
    12721 tgatcaacat tgtcgtgatt cttaacttaa cagtgggtat ctcgagttcg gtgcacatca
    12781 tttcgggcta caagtacttc aacgtgaccc tcgataagag acccgcgctg actaaagctt
    12841 atgaaaagtc gggtatatcg atcatgctcg cgacgctgac gaccctcgca ggtatgctgt
    12901 cgctcattgt cgtgcccatc gtgccgatca ggaattttgc ggtagcagcc tcatgcggca
    12961 tcctgttcgc ttatgttggt accattttcc tattgcctgc attgttgggt atctgggcac
    13021 cgggtgcctc gcgcgataag acaacgggcg aaaaaatgca gggactcttt gaacgccaga
    13081 ttcagcgttt cattgggttc atcatgaatg caacccacga caggccaaag ctcattatcg
    13141 gagtgttcag cgccatcatg gtgctcagcc tcaccgggta tccgcgcgtg atcgtcgata
    13201 cgaactttct ggagtcggta aagcccggct atggttttaa agaaaccttc gaaacgctgg
    13261 atcagcattt tggcggtaca accaacgtcg agattgtcat caataccggc aaaaacgacg
    13321 ggttgaaaga tgtgcagctt cttactgcca tggatcagct ggccgaacgg gcaaaaaaag
    13381 agcgacctga ttttgtaacg aaaacaacct cgctcgtaaa tgccgtaaaa gacgcgcata
    13441 agaacctgat gagccggggg ccgaacaagc cgggtacaga gtcgccctat tattccatac
    13501 ccgctgataa ccagaagctt aaacaggtgc tgctgctcta cgattcggcc gacccggaat
    13561 cgcgtaagtt attcgccgac gacgactggg aaaagggacg tatcactcta caggtcgtca
    13621 acaagagttc gtttgagtac gtcgaatttc ttaaacaggt agaaagctgg cttaaggaat
    13681 acctcgaacc tctcaagaaa agcaacgttg aactcagcta ttatgcaacg ggaggaattc
    13741 cgatcatgat gcggctgacc ggctttctga ccgagtcgca gatatcgtca ttcggcctgg
    13801 cactggtggt aatcacgatt atcatgtttc tgatttatgg tacgattcgg tatggcatga
    13861 tcgcgatgct gcccaatatc tttccgatct tgttgacggt cggcgtgacc ggctgggcga
    13921 aaattcccct cgactcagat actctgctcg tcctgcccat tgcgattggt attgctgtcg
    13981 acgataccat ccattttctc acgcactacc ggggcgaact ccacgcgggc cataaccgac
    14041 tcgaggccat caagactacc ttaagggagg tcggccaggc gatgattttt acttcagtga
    14101 ttctttcggt cggctttctg atttttctgc cctcggtcta cgtaccgttc agaaacttcg
    14161 gcatgctttc ggccatcgcc atttcaagcg cactttttgc cgacctgttt tttctgccgg
    14221 cgctgctcac ggtgttcggt aaatcgaata aagttaaagg agctcattca tgaaccagcg
    14281 aaatttactg cagaatataa cccgcgcgcg cagcgcacgg gttatattct gtctattatt
    14341 ctctgcgccg cttgcggcgc agaatctgaa agagcctgaa aaggcaaaac agatcgcgta
    14401 caagatgatt aagcgcgacg acggcaagac gtcgtataac cgcaccaagg tcatcacctg
    14461 cgcgttcgaa gagcagggcg gtaagcggaa gtgctcgggc caggcccgca ccaagtcgtt
    14521 tgagtcaatt tcgaaagatg tagggccgca gctcgaagac acgatcggtc tcagcgtgat
    14581 cgacgacccg gctgatgaga agggttcggc gttcctgcag aaggactacg aggtgccggg
    14641 caaagataca gaacagtgga tgtacctgcc ggcgctgcgc aagctca
//
LOCUS       DAOQJF010000033        14520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2002_length_14520_cov_5.682821, whole genome shotgun sequence.
ACCESSION   DAOQJF010000033 DAOQJF010000000
VERSION     DAOQJF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14520
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2002_length_14520_cov_5.682821"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLF85_03145"
     CDS             <1..240
                     /locus_tag="PLF85_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18683.1"
                     /translation="FKEDGTWIQTETLSSPYNDKVKGRMIPGNVQKACKKLGEPMMMG
                     PVTVTEGPKGSTYKLSCAGGDTVTFDKKAKVLKKE"
     gene            complement(299..679)
                     /locus_tag="PLF85_03150"
     CDS             complement(299..679)
                     /locus_tag="PLF85_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18684.1"
                     /translation="MTKGLFNGIAAVFAGFIAILLLSLFADFALLKTGLLAEGMNSVG
                     KFVLSISKEFIVGTIGAWFTVWLGRKHNLLHAAALAAVMIAIVLIPVSTNWGKNPLWF
                     SLTMLTLPVLYALAGVGVYRFLKK"
     gene            complement(735..1307)
                     /locus_tag="PLF85_03155"
     CDS             complement(735..1307)
                     /locus_tag="PLF85_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18685.1"
                     /translation="MSTTTATKQQGGISSASGARVYNARHSLDSLAEMTSAELENLYR
                     SAPAPESLNKVTGRPKGRMLTVDQTDGTPVFSGIKAFAAMNVFPWDGKSFAFEKGDKG
                     TGINRVKLLFANFDWFPFKTRIVPSAIDGKPCVFLDYEQPENPFFIARIHDEIREVSP
                     GVWMGPAMWKRDSANPLLILWFAVDFNQSR"
     gene            complement(1427..2515)
                     /locus_tag="PLF85_03160"
     CDS             complement(1427..2515)
                     /locus_tag="PLF85_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE18686.1"
                     /translation="MLGHFFIFSAFLATIQLVARLMVGNHSRRDRYLLLTLATLAYIL
                     YFAGTAIAPGVFPTAGLLHWHLPALFALGPSIHYFLKFSLLPDEDIKPKVILLQWLPA
                     ALCLMFIASPWYPQRHLSVDHLLQSYTTRPTDTHDLIAISAFAVNTGYYIWNVYETRF
                     LFRLNSPDFSGIFRYVALFQVGSVITPLLAMASLLTHEILLLKISCSLISLTIIGGYI
                     ASIRYPEFFLPLQVEAQKQKYQSSHLKNVDVKALAAKLDLLMREEKIYLDDKLSVKAL
                     ASLLQVTTHQLSEFINVHHGKNFSGLLNEFRIEEAKSLLSNEPAMSVIEVAFASGFGT
                     KSNFNSVFLKATGMSPLQYRRQNANATG"
     gene            complement(2516..3967)
                     /locus_tag="PLF85_03165"
     CDS             complement(2516..3967)
                     /locus_tag="PLF85_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18687.1"
                     /translation="MRVARAALGITTRLALTALLVACSVLPLQAQDNSTDEEDKQTFR
                     PIFDFLLREEPGISVSRKDSLLNPDDILGMPRLQNRVLASFDAKIPIVGGNNVNPIAE
                     LVVANTFVYLQHEGSEPTLAKQQIADYRNFLKEFYFNVRPTGNFLLTLGRRNLTDGVG
                     YSRNPVDFLANPSTLPGANFDNRFRLRNREGSIIARGEYLWKGGAVSYTYVPQLGPSD
                     ATDHKLRTEIDRLTQFNRSDAHWAKVYQLVAGYDLSLHYFYRQRHNIGLAVAKVFGDA
                     LELHAEGRAQRGSSVLTPYKRSEDVYIGPTLVQPALYDFEARPGETWYARVLVGGQYT
                     FENKLNLSTEYFYNGEGYSASQLATYANGLELAGSRYANSTFVLPAGNPYRLFLLAAN
                     QNFNFLSMGQHYVFMRLADPEFFGSNKWEAAVYASTLVTDGSGLAAGDLTYRINDRFS
                     LQFLVNGFFGTRRSEGGLFYQGMSALMALEAQF"
     gene            complement(3971..4693)
                     /locus_tag="PLF85_03170"
     CDS             complement(3971..4693)
                     /locus_tag="PLF85_03170"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNE18688.1"
                     /translation="MKKILFVVCLLTGALSAEDANSIVRKADAVRGPQGSFRMKIKVT
                     SERPDQAKQEMFLETYVKDRTTSVVKFTDPARERGKAMLMVNDDLWMYIPGSKRTFRI
                     SPQQRLMGDVSNADVARVNFADDYNSTMVKKSDKVDGKDCYQLELTAKTKVSYSKIHY
                     WVEHKTYRPVKATFFAVSGKPLKFATYTGYKTIAGSARPTLVHIVDGIKPDWKSEIHY
                     TSMGAKTFPDSIFQQSALPDLR"
     gene            complement(4703..5968)
                     /locus_tag="PLF85_03175"
     CDS             complement(4703..5968)
                     /locus_tag="PLF85_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE18689.1"
                     /translation="MSKLRIPTDIKIAFRNVFRNRRRTLLTLSSIVLGSAALIDFDGY
                     RNFADWGLREMYIRSQLGHIQIYKKGYRASKGMNPFAYLIPNSEDIKKTVADTLGDRL
                     KVVTARLEINGLITKGGQSFNFIAQGIEVEAERKMSALDDGTSSALKVRRGESLSDNE
                     AGSVLLGNGLCEALGVNDGDMLSLMGASAIGALNATDLVWKGCFSTVSKEYDKVAAIM
                     PLADAKRFSATEDVLKLVVMLKKTEMTDAAMRELQKAFADKGYDLEMVSWFELADYYK
                     QVMVFLSGMFMVIATIIVFVVVFSIINTLTMSVFERVQEIGTIRAMGAEGASIIRLFI
                     IEGIMLGLIGGAIGMASGGLVGVIVNNAKILLPPPPGGSESFLLQLRLYPSSFAMGFC
                     IAVGASFVASIYPAMKAAKLKVVDALRHV"
     gene            complement(5965..6705)
                     /locus_tag="PLF85_03180"
     CDS             complement(5965..6705)
                     /locus_tag="PLF85_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE18690.1"
                     /translation="MSLSGTSNDVVRLRAVQKSYPLGEYEVHALRGVDLNISEGEFTV
                     IMGPSGSGKSTLLNLLGCIDLPTTGEVEICGHNTTQKREKELADIRRNNIGFIFQAFN
                     LIPVLSVYENVEYALILKKVPEADRPALVDEIIEQVGLTAHKHHRPNQLSGGQRQRVA
                     IARALVTNPRLVLADEPTANLDSATGKSILELMRRMNEMHKTTFVFATHDPDVLSFAR
                     RQIRIHDGKIESDKGAAKTAATKRGKRK"
     gene            complement(6755..7678)
                     /locus_tag="PLF85_03185"
     CDS             complement(6755..7678)
                     /locus_tag="PLF85_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18691.1"
                     /translation="MAAQFLARTIKTPGPYDKPGASAFMVMSMAYVFFLPAVIGMVHV
                     WIRSREEAISYAKAIAEPLLPAILCLGISMLIGWEGTICLIMAIPVMLPLAMAGGLAM
                     RIILHLKNRNLVFAAFTIMPLPLAMAEQLVPLPQEMQVVRNSIVIEAPAEVIWRKIVR
                     VEKITEPQDSPFFSMGFPKPIEAVLSGDGVGQTRLATFERGLQFVEKVTDWKANEKLG
                     FSIEADPKATPLTTLDEHVTVGGAFFDVLSGTYEIHRLNARECELVLYSQHRISTHFN
                     FYARLWSRYLMSEIQWNILQVIKQRAEKEKA"
     gene            7886..8362
                     /locus_tag="PLF85_03190"
     CDS             7886..8362
                     /locus_tag="PLF85_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNE18692.1"
                     /translation="MEKPAGNVSREMALLKERMLQHQYKSTPQRDEIAEWVFRTHEHF
                     TVEELIASFRSKGKKVSQATAYRVIQMLLDLKLIEEHDFGKDYKFYEHTPGHEHHDHI
                     VCMDCGKIIEFADSSLETLKEKITLKNGFRMRKHHLTIYAECTTCPPEKRVLSNRQ"
     gene            8445..9143
                     /locus_tag="PLF85_03195"
     CDS             8445..9143
                     /locus_tag="PLF85_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18693.1"
                     /translation="MGDIGGIILSDGRQVYAGLHNSFTPQRDARKKKYLYIAMGIVLA
                     AELITYIAVSWQGEAREIPFSTMSIDLGEFKPRARRAPVIEVDEVFGNQFVKEKRADA
                     TVVPANEAGGSIDGEGVVDLDVEGGGSGGSGPNIARCAVRNFPPEAKAYVEEALVLVN
                     ILVDKTGLVRDAKPLYVSFKKNLPPELKERMKKLFMQAARTSLQGRRCPVYYAAGVAT
                     GYRLEVPLSYELYN"
     gene            9168..9734
                     /locus_tag="PLF85_03200"
     CDS             9168..9734
                     /locus_tag="PLF85_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18694.1"
                     /translation="MTKRNLLIVSLLAVVAALIFLFLKPCAPAEVGQANEGGFLPALK
                     NKLRKKPAAKETAPAEVPVTEAAPAPAAAPAEVDTAPPVNLSGSDEIPNVAKCVSANF
                     PTEAKPYVKTATVNVRLVVDKYGNVRSVKTTSVEFPEEPAEDMLPMLRKLFIQAGNRA
                     FGSKKCPPHVVNGQNVGYAIEVPLQYRH"
     gene            9742..10395
                     /locus_tag="PLF85_03205"
     CDS             9742..10395
                     /locus_tag="PLF85_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNE18695.1"
                     /translation="MKYQYFALDIDGTIFSSEHIIYPVYKESIELFCQERNTTLETPG
                     HERIMLEIGKPVKKIFQNLFPQLDERDRDFLSDSVLRLLCEKIAAGGGEYYPAVTETI
                     EAIVQRGGKILVASNGRRPYIEAILAYCKVLQHVIHPTYIDGQKIFTKSDIMRHYVSL
                     GIEPRSVVMVGDRLSDLEAAQAIDCDFAWCAYGHAPPGEITTWAIRLEQFLDLAQYA"
     gene            10395..10805
                     /locus_tag="PLF85_03210"
     CDS             10395..10805
                     /locus_tag="PLF85_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNE18696.1"
                     /translation="MPGSFSLHPQLEADTAPFRDLELSAVRVMDNASLPWLILVPRVD
                     DARELIDLTERQQQQLVREIAEVSQVLKMAFKPDKLNVAALGNMVPQLHVHVIARFSN
                     DLAWPKPVWGNLPAAPRSESEFEQFCALLNQYWR"
     gene            10890..11447
                     /locus_tag="PLF85_03215"
     CDS             10890..11447
                     /locus_tag="PLF85_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18697.1"
                     /translation="MKIISYLSAFMLLLFAAGCKSGQSQTWEGSVFTGQIDEQVDKLL
                     GGRDIYADPQRVVPVLVNSEQDSGAKKENLEGYVIKRKGKLLSPEQIKKLQAVIFDAN
                     TYDFKHSKRCMFVPYIGFIFEKSGKQAHALFCFTCNEVSFGRGGAQGNLEDFDAKRRE
                     ILQLARELFPDDARLAGLKEEGIGQ"
     gene            11444..12565
                     /locus_tag="PLF85_03220"
     CDS             11444..12565
                     /locus_tag="PLF85_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNE18698.1"
                     /translation="MRRLIVFAGIFSAATVLLAPLSAASLRVLIVGDTNDRSIGKSVV
                     TDVANFEAFARQVASRTGLTLDMKTIKGGDLKSKNITNAVNSLKADKEDAVIFYYSGH
                     GFRTQKVKTRWPLLYIPDAGQKGVDFQWVIDTINAKNPRMLLAISDSCNNFIDMPQAG
                     INSRAMLEDSDEAWRKLFVEFSGRIYASGSTVGQMSFGQDAVGGAFTSRFLSIVRAEV
                     KKADPKWDYIMQMATRQIYIGSPQQKTQDPQYELVKGAAVQQPQAEVTQAPQQAHEET
                     TSEREEECKSLSEFEAALVSVKDSMPVKLDFRRQRDDVKSYQEFVAALLQAGGDQQMV
                     KLAKTMDQGLKGRNWPRFRSAVWAYHNRVSKIHKDYCTQ"
     gene            complement(12591..>14520)
                     /gene="ppdK"
                     /locus_tag="PLF85_03225"
     CDS             complement(12591..>14520)
                     /gene="ppdK"
                     /locus_tag="PLF85_03225"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNE18699.1"
                     /translation="VAFTRNPANGERKFFGEFLMNAQGEDVVAGIRTPLSIEKLGEKM
                     PEALKELLSYADKLEHHYKDMQDIEFTIERSKLYMLQTRSGKRTAFAAIRTAVEMVDE
                     GLIDEKTAIKRIDASTLPSLLAKIFDEKEKKEVLAKGLVVASGLPAGPGAASGHIALT
                     AEKAVEMEKKGVKSILVRVETSPEDISGMHSSEGILTARGGMTSHAAVVARGMNKPCI
                     VGCSALEVDYKTGTIEFNAGTDKEVKLKEGDHISIDGFTGQVLNAALKTIPSELEQVF
                     VSKTMPVEKSVLAQQYARIMQWADKYSRLKVRTNADTPEDAAVARSYGAQGIGLCRTE
                     HMFFAEERIAAMREMILSSTLEEREKALAKLLPYQRADFKGIFAAMEGLPVTVRLLDP
                     PLHEFLPHEEAQIEELAKAFGKSIQYVHKKIGDLKEFNPMLGHRGCRLGVTYPEITAM
                     QTRAIIEAAAELIKEGKKVYPEIMVPLVGHHKELANQAKVIREIADKVQKEKGVTVEY
                     SLGTMIEVPRAAVTADEIAEHAQFFSFGTNDLTQMTSGFSRDDSEAFLPYYIEKGIYP
                     QNPFQVLDAGGVGKLIEIAIEKGRSVNAKIKLGVCGEHGGDPASIGFCHRVGLEYVSC
                     SPYRVPIARLAAAQAALA"
BASE COUNT         3219 a         3898 c         4131 g         3272 t
ORIGIN      
        1 ttcaaagaag acggcacgtg gatacagaca gaaacactgt cttcaccgta taacgacaag
       61 gtgaaagggc gcatgatacc cggcaatgtg cagaaggcct gcaagaagct gggcgaaccg
      121 atgatgatgg gacctgttac tgttacagag gggcctaaag gcagcaccta taaactgagc
      181 tgtgctggcg gagatacagt gaccttcgat aagaaagcaa aggtactcaa gaaagaataa
      241 ccaaatctca atgacaacgc agtgtctgcg gatctgtgcc cgcagacact gtttaggatt
      301 acttttttag aaaccggtaa actcccaccc ctgcgagcgc ataaagcacg ggtaatgtaa
      361 gcatcgtcag tgaaaaccac agggggttct taccccagtt ggtgctgaca ggaatcagca
      421 caatggcgat catcacggcc gcgagtgctg cagcgtggag caggttgtgc ttgcgaccga
      481 gccagacagt aaaccacgcg ccgattgtgc cgacgatgaa ttctttcgag atcgacagca
      541 caaatttgcc gacggagttc atgccttctg caagaagtcc tgtcttgagc agggcaaaat
      601 ctgcaaaaag agaaagcagc agtatcgcga tgaaacccgc gaagacggca gcgatgccgt
      661 tgaagagtcc tttggtcatg aggacaaaat acaattcctt ttgggcgtgc aacgcccaaa
      721 aggaaccctg acacttagcg tgactggttg aagtcgactg cgaaccagag tatcagcagg
      781 gggttcgctg agtcgcgctt ccacatcgcg ggacccatcc agacgcccgg cgacacctcg
      841 cgaatctcat catggatgcg cgcgatgaag aacgggttct cgggttgttc gtagtcgagg
      901 aacacgcagg gcttgccgtc gattgccgat ggcacaatgc gcgtcttgaa ggggaaccag
      961 tcgaagttgg caaacagcag tttcacgcgg ttgatacctg tgcccttatc gcctttctca
     1021 aaagcaaagc tcttgccgtc ccatgggaag acgttcattg ccgcgaaagc tttgatgccg
     1081 ctgaagaccg gtgtgccgtc ggtctggtcg acagtcagca tgcggccctt cgggcggcct
     1141 gtcaccttgt tgaggctctc gggtgccggg gctgaacggt agagattttc gagttcggcc
     1201 gatgtcattt cggcgaggct gtcgagcgag tgccttgcat tgtagacgcg tgcccccgaa
     1261 gccgaagaaa ttccgccttg ttgctttgtt gctgttgttg tgctcataga agagactcct
     1321 tatgagtggc atatagtggc cgcctcatgc gcctttttta gcgcaatttc agaagaaaat
     1381 cgaccggaat tataaatttg ggcacaaaaa aggcagaagg gccaggttaa ccagtggcat
     1441 tcgcgttttg ccggcggtac tgcagcggtg acatgccggt ggctttgaga aagacggagt
     1501 taaagttcga tttcgtgccg aaaccggaag cgaaggccac ttcgatcacg ctcatggctg
     1561 gttcgtttga aagcagcgac tttgcctctt cgatgcggaa ttcgttcagc agacccgaga
     1621 aattcttacc atggtgaacg ttgatgaatt cagaaagctg gtgcgttgtc acctgcagca
     1681 ggctggcgag ggcttttact gaaagtttat cgtcgaggta gattttttct tcacgcatga
     1741 gcaggtcgag tttggcggcg agggctttga cgtcgacgtt cttcaggtgt gaagactggt
     1801 acttctgctt ctgagcctcg acctgtaggg gcaaaaagaa ctcggggtag cgtattgaag
     1861 cgatgtagcc accaattata gtcagcgaaa tcagcgaaca gctgatcttc agtagcagaa
     1921 tctcgtgcgt caaaagcgat gccatcgcca aaagcggagt gattaccgaa cctacctgga
     1981 acagcgcgac atagcgaaag atgccgctaa agtcgggtga gttcagccga aacaaaaacc
     2041 gcgtctcgta gacattccag atgtagtagc cggtgttcac ggcaaaggcc gagatggcga
     2101 tcaggtcgtg cgtgtcggtg ggtcgcgtgg tatagctctg cagcaggtgg tcgacgctga
     2161 ggtggcgctg tgggtaccag ggtgaggcga tgaacatgag gcagagtgcc gcgggcagcc
     2221 actgcagcag aatcaccttt ggcttaatat cctcgtcggg caacagcgag aatttcagga
     2281 agtaatggat cgagggccca agcgcaaaaa gcgccggcag gtgccagtgc aacaggcccg
     2341 ccgtgggaaa aacgcccggc gcgatcgcgg tgccggcgaa atacagaatg taggccaacg
     2401 tcgccagggt gagcagcagg taacgatcac gccggctatg gttgccgacc atgagacgcg
     2461 ccaccagctg tatggttgca aggaaggcgg aaaaaataaa gaagtgcccg agcatctaaa
     2521 actgcgcctc gagcgccatc agcgctgaca taccctggta gaacaggccg ccctcgctgc
     2581 ggcgcgtgcc gaagaaaccg ttcacgagaa actgcaggct gaaccggtca ttgatgcggt
     2641 aggtcaaatc gcctgccgcg agtcccgagc cgtctgtgac aagggtcgat gcatacactg
     2701 cggcctccca cttgtttgag ccaaagaact cggggtcggc gaggcgcata aagacgtagt
     2761 gctggcccat cgaaagaaaa ttgaagttct ggttcgccgc cagcagaaat aaccggtacg
     2821 ggttgccggc tggcagcacg aacgtgctgt tcgcataacg actgcccgcg agttcaagcc
     2881 cgttcgcata ggtcgcgagc tgcgaggctg aataaccttc gccgttataa aagtactcgg
     2941 tcgaaaggtt gagcttgttt tcaaaagtgt attggccgcc aacgagcacg cgcgcatacc
     3001 aggtctcgcc ggggcgggcc tcgaagtcgt agagcgcggg ctgaacgagt gttggcccga
     3061 tataaacatc ttcagaacgt ttgtagggag tcagcacaga actgccgcgc tgcgccctgc
     3121 cttcggcatg cagctcgagt gcgtcgccga acaccttggc gacggcaagg ccgatattgt
     3181 ggcgctgccg gtagaaatag tgcagcgaga ggtcataacc ggcgacgagc tggtagactt
     3241 ttgcccagtg cgcgtcgctg cggttaaact gggtgagccg gtcgatctcg gtgcgcagct
     3301 tgtggtcggt ggcgtcactt ggccctaact gtggcacgta cgtgtatgag acagctcccc
     3361 ctttccagag gtattcgccg cgcgcgatga tgctgccctc gcggtttctc aggcgaaacc
     3421 ggttgtcgaa gttggcgccg ggcagcgtcg acgggttggc gagaaagtcg accgggttgc
     3481 gcgagtagcc gacgccgtcg gtgaggttgc gccgcccgag ggtgagcaaa aaatttccgg
     3541 tggggcgtac gttgaagtaa aactctttca gaaagttgcg gtagtcggca atctgctgct
     3601 tcgccaacgt cggctcgctg ccctcgtgct gcaggtagac aaacgtgttg gcgacgacca
     3661 gttcggcgat cgggttgacg ttgttgccgc cgacgatcgg aatcttcgca tcaaaactcg
     3721 ccagcacgcg gttctgcaaa cgcggcatac ccaaaatatc atcggggttc agcaggctgt
     3781 cctttctgct gacagaaatg cccggctctt cacgaagcaa aaaatcaaag ataggcctga
     3841 aggtctgctt gtcttcttcg tcggtgctgt tatcctgcgc ctgcagcggc agcacactgc
     3901 aggcaaccag caacgcggtg agtgcgagtc tcgttgttat accaagtgcc gcccgggcga
     3961 cgcgcacgcg ttacctcagg tcgggcagcg ccgattgctg gaaaatactg tcgggaaaag
     4021 ttttcgctcc catcgacgtg taatggattt cgctcttcca gtcgggtttg atgccgtcga
     4081 cgatgtgtac cagggtcggc cgcgcagagc ccgcgatcgt cttataaccg gtgtacgttg
     4141 cgaacttcag tggcttgccc gagacggcaa agaaggtggc cttcacgggg cggtaggtct
     4201 tgtgttcgac ccagtaatgg attttcgaat aactcacctt ggttttggca gtgagctcca
     4261 actggtagca gtctttgccg tccaccttgt cgctcttctt gaccatggtg gagttatagt
     4321 cgtcggcgaa gttgacgcgt gcaacgtcgg cattcgatac atcgcccatc aggcgctgct
     4381 gcggtgaaat acgaaacgtg cgctttgaac ccgggatata catccacagg tcgtcgttaa
     4441 ccatcagcat cgccttgcca cgctcgcggg cagggtcggt gaattttacc accgaggtgg
     4501 tgcggtcttt gacatacgtt tccaaaaaca tctcttgttt tgcctggtcg ggtcgttcag
     4561 aggtgacctt gatcttcatg cgaaacgaac cctgtggccc gcgtacggca tctgccttgc
     4621 gcacaatgga gtttgcatct tctgcggaaa gagcgcctgt aagcaggcaa accacgaata
     4681 gtattttttt catttgttct ccttaaacat gccgcagcgc atcgacaact ttcagcttgg
     4741 ccgctttcat tgccgggtag atcgaggcga cgaaactggc gccgaccgcg atacagaagc
     4801 ccatggcaaa actcgacggg taaagcctta actgcagcaa gaagctttcg ctgccccccg
     4861 gtggcggcgg tagcagtatc tttgcattgt tgacaatcac gccgacgaga ccacccgagg
     4921 ccatgccgat ggctccgccg attaacccca gcatgatacc ctcgataata aagaggcgga
     4981 tgatcgacgc accttcggcg cccatggccc tgatcgtgcc gatctcctgc acgcgctcga
     5041 ataccgacat ggtcagcgtg ttgataatcg agaagaccac aacaaagaca atgatggtcg
     5101 caatcaccat gaacatgccg ctcaggaaaa ccatgacctg cttgtagtag tcggcaagct
     5161 caaaccagct gaccatttcg aggtcataac ctttatcggc gaatgccttc tgaagttctc
     5221 tcatcgcggc atcggtcatt tcggtcttct tgagcatgac cacgagtttc aatacatctt
     5281 cggtggcgga aaaccgtttg gcatcggcaa gcggcatgat cgctgccacc ttgtcgtatt
     5341 ctttcgacac ggtcgagaag cagcctttcc agaccaggtc ggtcgcgttc agcgccccaa
     5401 tcgcactcgc gcccatcagg ctcagcatgt cgccgtcatt cacgccgagt gcttcgcaca
     5461 gcccgttgcc cagcaaaacg gaaccggctt cattgtcgct cagcgactcg ccacgacgca
     5521 ccttcagtgc gcttgaagta ccgtcgtcga gggctgacat cttgcgctct gcttcaactt
     5581 cgattccctg cgcgatgaag ttaaatgact ggccaccctt ggtaatgagg ccattgattt
     5641 cgaggcgagc ggtgacgacc ttcaggcggt cgcccagggt atcggcaacg gttttcttga
     5701 tatcttctga attgggaatc aggtaggcaa agggattcat acccttcgaa gcccggtaac
     5761 ctttcttata gatctgtatg tggcccagtt gcgaccggat atacatctcg cgcaggcccc
     5821 agtcggcgaa gttgcggtaa ccgtcgaagt cgatcagcgc cgcagagccg agtacaatcg
     5881 acgatagggt cagcagcgtg cgccggcggt tgcggaagac gttgcggaat gcgatcttga
     5941 tgtcggtggg gattcgcagc ttactcattt gcgtttacct ctttttgtgg cggcggtttt
     6001 tgccgcaccc ttgtcgcttt cgatcttacc gtcgtgtatg cgtatctgcc gtctcgcgaa
     6061 cgagagcacg tcggggtcgt gcgtcgcgaa cacaaacgtg gtcttatgca tttcattcat
     6121 gcgccgcatg agttcgagaa tcgatttgcc cgtagctgaa tcgaggttgg cggttggttc
     6181 atcggccagc accagccgcg ggttcgtcac gagcgcgcgg gcaatcgcga cgcgctgtct
     6241 ttgcccccct gaaagctggt tgggccggtg gtgtttgtgc gcagtgaggc ccacctgttc
     6301 gatgatctcg tcgacgagcg ccggccggtc ggcttcgggc acctttttga gtatcagcgc
     6361 gtactccacg ttttcgtaga ccgaaagcac ggggatcagg ttaaacgcct ggaagatgaa
     6421 gccgatgttg ttgcggcgaa tgtctgcgag ttctttttcg cgcttctgcg tcgtgttgtg
     6481 cccgcagatt tcgacttcac ccgtcgttgg caggtctatg cagcccagca ggttcagcag
     6541 cgtactcttg cccgaaccag agggccccat gataacggtg aattcacctt cagaaatgtt
     6601 gagatcgaca ccgcgtagcg cgtgcacctc gtattcgccg agcgggtatg acttctgcac
     6661 ggcgcgtaag cgcactacat cgttgctggt acctgaaagg ctcataacgg cctttctatg
     6721 caggccgaga atgcgtaaag atgtatccgc ccggtcaggc tttttctttt tccgcgcgct
     6781 gcttgatgac ctgcagtatg ttccactgta tctcgctcat caggtagcgg ctccagagac
     6841 gcgcgtaaaa attaaaatgg gttgaaatgc ggtgctggct gtagagtaca agttcgcatt
     6901 ccctggcatt tagccggtgg atttcgtaag tacccgagag tacgtcgaag aaggcgcccc
     6961 cgacggtgac gtgctcgtcg agagtcgtca gcggggtcgc cttcggatct gcctcgatgc
     7021 taaagccgag tttctcattc gccttccagt cagtcacctt ctcgacgaac tgcagacccc
     7081 gttcgaacgt tgccagccgc gtctgaccga caccgtcgcc tgagagcaca gcttctatcg
     7141 gtttaggaaa tcccatgctg aaaaaaggac tgtcctgtgg ttcggtaatt ttttcgaccc
     7201 gcactatttt gcgccagatt acttcggccg gggcttcgat cacaatggag tttctcacga
     7261 cctgcatttc ctgtggcagt ggtaccagct gctcggccat agccagcggc agtggcatga
     7321 tggtgaatgc ggcaaagacc aggttcctgt tcttcaggtg gaggataata cgcattgcca
     7381 gcccgccggc cattgcaagt ggtaacataa caggtattgc catgatcagg cagatcgtgc
     7441 cttcccagcc gatgagcatc gaaataccca ggcacaggat tgccggcaaa agaggttctg
     7501 caattgcttt ggcgtaagag atggcttctt cgcggctgcg tatccagacg tggaccatac
     7561 cgatgacagc cggcaggaaa aaaacatagg ccatcgacat taccatgaac gccgaggcac
     7621 ctggcttatc gtaggggccc ggcgttttga tcgtgcgtgc aagaaactgc gctgccacgc
     7681 cataaataac gccaatgcag gcggcgaaaa acatcgaacg gttcaatggg cgttgtggca
     7741 gtcgaatcat gggacagcag tccaagcagt cgtcagaaag caatctcttt tgccggaatc
     7801 actgcacggg tattttgtca gccaaatgga gactattcga taaagcttga cgaagcagtt
     7861 ttgtatgggt attcttacac cacctatgga aaagcccgcg ggcaatgttt cacgcgagat
     7921 ggctctgctg aaagagcgca tgctccagca ccagtataaa tccaccccgc agcgcgacga
     7981 aattgccgag tgggtgtttc gtacccacga gcactttact gtcgaagaac tgatcgcgag
     8041 ttttcgctcc aagggcaaaa aggtttcaca ggccacggcg taccgggtta tccagatgct
     8101 gctcgatctc aagctgatcg aagaacacga ctttggcaaa gactacaagt tctacgagca
     8161 tacaccgggt cacgagcacc acgaccacat cgtctgcatg gattgcggca agattattga
     8221 gtttgccgat tcgagcctcg agacacttaa agaaaagata accctcaaaa atggttttcg
     8281 catgcgcaag caccacctga ccatctatgc cgagtgcacg acttgcccgc cggaaaagcg
     8341 ggtcctgagt aaccggcagt agttcttcac cggtatccgg attctgttca caccagcagg
     8401 gccaaaaatc ttgtccgcct gatttgccct cctgccgtgg tgatgtggga gacatcggcg
     8461 gcattatact ttccgatggg cggcaggtct atgctggcct gcataactcg ttcacaccgc
     8521 agcgcgacgc gcgcaagaag aaatacctct atatcgccat gggtatcgtg ctggcggccg
     8581 aactcatcac gtatattgcg gtgagctggc agggcgaagc gcgcgagatt cctttctcga
     8641 cgatgtcgat tgacctgggc gagttcaaac cccgggcacg gcgcgcgccc gtgatcgagg
     8701 tcgacgaggt tttcggtaac cagttcgtca aagagaagag ggcagatgcg accgttgtgc
     8761 ctgccaatga agcgggtggc tcgatcgacg gcgagggtgt cgttgacctc gatgtcgaag
     8821 gcggcggatc aggcggcagc ggacccaaca ttgcacgctg cgcggtacgc aattttcctc
     8881 ccgaggcgaa agcttacgta gaagaagccc ttgtgctggt aaacatcctg gtcgacaaga
     8941 cggggctcgt gcgcgacgca aaacctcttt atgtcagctt caagaagaac ctgccacccg
     9001 agctgaaaga gcgcatgaag aagctgttca tgcaggcggc acgcacttct ctgcagggac
     9061 gccgctgccc ggtttattat gctgctggcg ttgctacggg ctaccggctc gaagttccgc
     9121 tgagttatga gctttacaac tagccgaccg ctctgaaaca ccctgcaatg accaagcgca
     9181 atctgctcat cgtctcactg cttgctgttg tcgcagcgct cattttcctg tttttgaaac
     9241 catgtgcacc tgccgaagta ggccaggcca atgagggggg atttctgccc gcattgaaga
     9301 ataagctgcg taaaaagccg gcggcaaaag aaacggcacc ggctgaggtt cccgtaacgg
     9361 aagccgctcc ggcgcctgct gcggcaccgg ccgaagtcga tactgcaccg cccgttaacc
     9421 tgagtggcag cgacgagatt cccaatgtcg ctaagtgcgt gtcggcgaat tttcccaccg
     9481 aagcaaagcc gtatgtaaag acggccaccg tcaacgtgcg cctggtcgtc gacaagtatg
     9541 gcaacgtgcg ctcggtcaaa acaacttctg tcgaatttcc tgaagagccg gccgaagaca
     9601 tgctgccgat gttgcgtaag ctctttatac aggcaggcaa ccgcgcgttc ggctcaaaga
     9661 agtgcccgcc gcacgtggtc aatggccaga atgtcggtta tgccatcgaa gtgccgctgc
     9721 aatacaggca ctgacaagac tatgaaatac cagtactttg cgctagatat cgacggcacc
     9781 attttttcct ccgaacatat catctacccg gtctataaag aatcgattga gcttttctgc
     9841 caggagcgca ataccacgct cgaaacaccg gggcacgagc gtatcatgct cgaaattggc
     9901 aaaccggtga agaaaatctt ccagaacctt ttcccacagc tcgacgaaag ggaccgcgat
     9961 tttctctctg attcagtgct gcggctgcta tgcgaaaaga ttgctgccgg cggcggggag
    10021 tactatcctg ccgtgacaga aacgattgag gcgattgtac agcgcggcgg aaagattttg
    10081 gtcgcttcga acggccggcg tccgtacatt gaggccattc tggcttactg caaggtgctg
    10141 cagcacgtga tacatcccac ctatattgac gggcagaaaa ttttcaccaa gagcgatatc
    10201 atgcgccatt acgtttcgct cggtatcgag ccgcgttcgg tcgtcatggt gggagaccgc
    10261 ctcagcgatc tcgaagctgc gcaggcgatt gactgcgact ttgcctggtg cgcgtacggc
    10321 cacgccccgc ctggcgaaat tacgacctgg gcaattcggc tcgagcagtt tttagacctt
    10381 gcacaatacg cctgatgcct ggcagttttt ctctgcatcc ccagctcgaa gccgacacgg
    10441 cgccctttcg cgaccttgaa ttatctgccg tacgcgtcat ggataacgcg tcgctgccct
    10501 ggttgattct tgtcccgcgt gttgatgatg cgcgtgaact gatcgacctc accgaaaggc
    10561 agcaacagca actggtccgc gagatcgctg aagtatcgca ggtgttgaag atggcattta
    10621 aacccgacaa gctcaatgtg gctgcgctgg gtaacatggt gccgcagctg cacgtgcacg
    10681 tcatcgcgcg tttcagtaac gatttggcct ggccgaagcc tgtctggggt aacctgcctg
    10741 cagcaccgcg ctccgaaagt gagttcgagc agttttgcgc actgctgaat caatactggc
    10801 gctagaacca tcacctcctc tggccatttt ttacgaacta ccagagaaac aataccttga
    10861 ccacagaggt aactggtgtt ttatgcgcca tgaaaattat cagctatttg agtgcgttta
    10921 tgcttttgct ttttgcagca ggctgtaaat cgggccagag ccagacgtgg gagggatcag
    10981 tgtttacggg acagatcgac gagcaggtgg acaaacttct tggcggcagg gatatctacg
    11041 ccgacccgca gcgggttgtt cccgtgctgg tgaattcaga acaggattct ggcgccaaaa
    11101 aagaaaacct tgaagggtat gtaatcaagc gcaagggcaa actgctctcg cccgagcaaa
    11161 tcaagaagct gcaggcagtt atttttgacg cgaataccta tgattttaaa cactcgaagc
    11221 gctgcatgtt tgtgccttat atcggtttta tctttgagaa gtctggcaag caggcgcatg
    11281 cgctgttctg ttttacgtgc aacgaggtga gtttcgggcg tggtggagcg cagggtaacc
    11341 ttgaggattt cgacgcgaaa cgccgtgaaa ttctgcagct cgcccgcgag ctttttcccg
    11401 atgatgcacg cctggcgggc ttgaaagaag agggtatcgg tcaatgagac ggttgattgt
    11461 ttttgcgggt attttctctg cagccacagt tctgctggcg ccgctttctg cggcatcgct
    11521 gcgcgtcctt attgtcggcg atacgaatga ccggtcaatc ggcaagtctg tcgtgaccga
    11581 tgtggcaaat ttcgaggctt tcgcgcgcca ggtcgccagc cgcaccgggc ttacgctcga
    11641 catgaagact atcaagggcg gcgatctcaa atccaaaaat atcaccaacg ccgtgaacag
    11701 cctcaaggca gataaagaag atgccgttat tttctattac tcggggcacg gtttccggac
    11761 acagaaggtt aagactcgct ggccgctgct ctatattccc gatgcgggcc agaagggcgt
    11821 agatttccag tgggtcatcg ataccataaa tgccaaaaat ccccgcatgt tgctggcgat
    11881 tagcgattcg tgcaacaact tcatcgatat gccacaagca ggtattaatt cgcgggccat
    11941 gcttgaagac tcggacgaag cgtggcgtaa gctgttcgtc gaattctccg gccgcatcta
    12001 tgcgtcgggc tcgaccgtgg gccagatgtc ttttgggcag gatgcggtgg gcggcgcctt
    12061 taccagccgt tttctttcga ttgtccgtgc cgaagtaaaa aaggccgacc ccaagtggga
    12121 ctacatcatg cagatggcaa cacgtcaaat ctacattggt agcccgcagc agaagacgca
    12181 ggacccgcaa tacgagctgg tcaagggtgc cgcggttcag cagccgcagg cagaggtgac
    12241 gcaggcgccg caacaggccc acgaagaaac cacttcagag cgcgaagagg agtgtaagtc
    12301 ccttagtgag tttgaggcag cgctcgtgag cgtgaaagac tctatgcccg tcaaactcga
    12361 tttcaggcgc cagcgcgatg acgtaaaatc ataccaggag tttgttgcag cgctactgca
    12421 ggcgggaggc gaccaacaaa tggtcaagct ggcaaagacg atggaccagg ggctgaaagg
    12481 ccgcaactgg ccacgcttcc gttcggccgt ctgggcatac cacaaccgcg tttcaaagat
    12541 ccacaaagat tactgcacgc agtaaccttt gtgtttagtc aggctgcgct tcaggccagg
    12601 gcagcctgcg ccgcagccag gcgcgcgatc ggcacccggt agggcgagca cgatacgtac
    12661 tcgagaccca cgcggtggca gaaaccgatc gaagcgggat ctcccccgtg ttcgccgcag
    12721 acaccgagct tgattttcgc gttcacgctg cggccctttt cgatcgcaat ttcaatcagt
    12781 tttcccacgc cgccggcatc gagtacctgg aacgggttct gcgggtagat gcctttctca
    12841 atgtagtaag gcaggaaggc ttctgaatcg tcgcgcgaga agcccgaagt catctgcgtc
    12901 aggtcattgg tgccaaagct gaaaaactgc gcatgttcgg caatttcatc tgcggtcacc
    12961 gcggcgcgcg gcacttcgat catggtgccc aggctgtact caactgtcac acctttctct
    13021 ttctgtactt tgtcggcaat ttcgcggatc acttttgcct ggttcgcgag ctctttgtgg
    13081 tggccgacca gcggcaccat aatttcagga tagaccttct tgccttcttt tattagttca
    13141 gctgctgctt cgataatcgc gcgcgtctgc atcgcggtga tttcggggta cgtcacgccg
    13201 aggcggcagc cacggtggcc gagcatcggg ttaaactctt tgaggtcgcc gatcttcttg
    13261 tgcacatact gtatcgattt gccaaaagcc ttcgccagct cttcgatctg cgcttcttcg
    13321 tgaggcagga attcatgcag tggtgggtcg agcaggcgca cggtgacggg taaaccttcc
    13381 atggcagcaa agataccctt gaagtcggcg cgctggtaag gcagcagttt tgccagcgcc
    13441 ttttcgcgct cttcgagagt tgaagagaga atcatttcac gcatcgcagc gatacgctct
    13501 tcggcgaaga acatatgctc ggtacggcaa aggccaatgc cctgcgcgcc gtaactgcgt
    13561 gcgacggccg catcttcagg tgtatcggcg ttcgtgcgca ccttgaggcg gctgtatttg
    13621 tctgcccact gcatgatgcg cgcgtactgt tgcgctagaa ccgatttttc caccggcatc
    13681 gtcttactga caaaaacctg ttcgagttcg cttggaatgg tcttgagagc agcattgaga
    13741 acctgcccgg taaaaccatc gatcgagatg tggtcacctt ctttgagttt gacttctttg
    13801 tcagtaccgg cgttgaattc tatggtgcct gtcttgtaat cgacttcgag ggcactgcag
    13861 ccaacaatac atggcttgtt catgccgcgc gcgaccaccg cggcatgcga ggtcataccg
    13921 ccgcgtgctg tcaggatacc ttcagagctg tgcatgcccg agatgtcttc gggtgaagtt
    13981 tcaacccgca ccaggatcga ttttacgcct tttttctcca tctcaactgc tttttcggct
    14041 gtcagtgcaa tgtgaccgct cgcagcgcca ggacccgcgg gcagacccga ggcaacaacc
    14101 agccccttag cgagcacttc tttcttttct ttctcgtcga agatcttggc gagcagcgag
    14161 ggcagcgtgc tcgcatcgat gcgcttgatt gccgtttttt cgtcgatgag gccttcgtct
    14221 accatctcga ccgcggtacg tattgcggcg aatgcagtgc gcttgcccga gcgagtctgc
    14281 agcatataca gttttgacct ttcgatggta aactcgatat cctgcatgtc cttgtagtgg
    14341 tgctcgagtt tatccgcata gctgagtagt tctttgagcg cttcaggcat tttttccccg
    14401 agcttttcaa tcgagagtgg agtgcgtatg cctgcaacca cgtcttcacc ctgtgcgttc
    14461 atcaggaact ccccaaagaa cttgcgctcg ccgttggccg ggttacgcgt aaaggcaacg
//
LOCUS       DAOQJF010000034        14518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2004_length_14518_cov_5.125147, whole genome shotgun sequence.
ACCESSION   DAOQJF010000034 DAOQJF010000000
VERSION     DAOQJF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14518
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_14518_cov_5.125147"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLF85_03230"
     CDS             complement(<1..319)
                     /locus_tag="PLF85_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18700.1"
                     /translation="MRIPRIILKYSALPAVTAIGIALVALLAATIVGVETYSDRQQGF
                     SAEADKIQLKINERLNAYALILRGAAGLFDASDRVTRRDWKRYVESLRPNDSVPGVQG
                     IGYS"
     gene            complement(437..1060)
                     /locus_tag="PLF85_03235"
     CDS             complement(437..1060)
                     /locus_tag="PLF85_03235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE18701.1"
                     /translation="MNSAGKAFEQWDERYQSEQYFYGTEPNDFLKSQAHRLLPGASVL
                     CLAEGEGRNAVYLAKQGFSVTAVDGSAQGFVKLQKLAMQTGVHVRTVVKDLAHFEMGH
                     RQYDAIVSVWCHLPQPLRAEIHRRVVVALKPGGFFILEAYTPRQLQYKTGGPPNATML
                     MTRNLLKTELAGLEVEIAQELDREIHEGKGHNGKSAVVQFVARKPRR"
     gene            1157..1654
                     /locus_tag="PLF85_03240"
     CDS             1157..1654
                     /locus_tag="PLF85_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNE18702.1"
                     /translation="MSYSSEFADRIRKILSSQTTFVEKEMFGGVAFMVDDKMCVGINQ
                     DKRTGVDRLMARIDPEFYYSALKREGCREMDFAGRPMIGFVFVHPEGYANEEDLVFWI
                     GKSLEYNRVVPVSTVKKKRALLAPPEKKIRTVKPLPKYKPPKAKTVKSPAAAGTKKVV
                     ANKKK"
     gene            1696..2286
                     /locus_tag="PLF85_03245"
     CDS             1696..2286
                     /locus_tag="PLF85_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18703.1"
                     /translation="MKILKTLLVIIVILAGAITGIYAWLGGFSTVAVNRGEIGPWEIV
                     YSTHKGPYKNLNKSWTKFQKDWEAAGLKVCNSLAVYLDMPGTPEDQLRSAIACRIDEL
                     APAEKAALRGKLPAFVIPKSRAVMATFPYKNPASYFVGPMKVYPAFHKVMTQQKLLAP
                     VGIETYGATNKPAEQIGYAMPVDSKRSDYQKLIDSF"
     gene            2398..6162
                     /gene="metH"
                     /locus_tag="PLF85_03250"
     CDS             2398..6162
                     /gene="metH"
                     /locus_tag="PLF85_03250"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNE18704.1"
                     /translation="MPLYSETEIRQALAERILILDGAMGTMIQRRSLTEEDYRGTGSV
                     SPVELWKSMAPDFQVPAEVWNSIAKSEIPLKGNSDLLCLTRPDVIAAIHREYMDAGAD
                     IIETNSFSSNFISQADYKLEPLVSVLNEAAVRCVAFARQEYYKTNGESRKIFIAGSLG
                     PTTKTASISPDVNNPAYRATTFDELKAAFKEQTVALLSAGSDLLLPETNLDTLNVKAA
                     IVAFEEAFEEVGYRVPVSISVTITDASGRTLSGQTAEAFFTSVRHANPLSIGINCALG
                     AKDMKPHLQALSRVSDVMISCYPNAGLPNAFGGYDETPAMFASDLAVFAKEGLLNIAG
                     GCCGTTPDHIKEMVKRVATEKPRAVAGLDARKGLSLSGLEPLNVDPSTPLRAGGSQFL
                     MIGERTNVTGSPKFKKLILEDKFDEALDVARQQVIAGANIIDVNFDEALLDGEACMTR
                     FLNLIASEPDIARVPIMIDSSKWSVIYAGMKCIQGKGIVNSISLKEGEEKFLEQARTV
                     HEHGFAMVVMAFDEQGQAASEDDKVRICKRAYKLLTEKAHVPPSDIIFDPNILTVATG
                     IEEHNNYALDFINATRRIKQECPGAYVSGGVSNVSFSFRGNNAVREAIHSVFLYHAIK
                     AGLDMGIVNAGMLAVYEDIPPELREHVEDVILNRRPDSTERLLAIADKYKSGGKEQVK
                     EDLSWRNGPPEQRIEHALVKGIDAYVEEDVEELRRKYQPTLRVIEGPLMGGMRVVGEL
                     FGAGKMFLPQVVKSARVMKKAVAYLLPFMEAEKAAAGTSSAQKKVLMATVKGDVHDIG
                     KNIVGVVLACNNYDVHDMGVMVPCDKILEKAREINADVIGLSGLITPSLDEMVYVASE
                     MQKGGFTQPLLIGGATTSAAHTAVKIVPAYNNAVVHVPDASLVTGVLGNLLSEDLKPA
                     YVRQLSAEQTKVREDYLSGKKERQFLAFADARAHAPQYKPAPVKLPNKLGITLLDNVR
                     LGDLVPYIDWSPFFMAWELRGRYPRILEDEVVGKEATKLFNDAQKILTQILRDKPFTP
                     RGVVGIFPCRREGDDVIVYADENRDKVLHKLHFLRQQKKKENDQIYYSLADFIGEQDY
                     IGGFAVTAGDGAGEYAARFEKQNDDYSAIMVKALADRFAEAFAEYAHEIVRKHTWGYA
                     ADEKLDIEGLIKEEYQGIRPAPGYPASPDHTEKRTLFDWLSVEKNTGIQLTESFAMTP
                     PSSVSGLYFAHPEAKYFAVGKIDRDQLEDYAARKGKPVDEMERWLRPYLD"
     gene            complement(6170..7378)
                     /locus_tag="PLF85_03255"
     CDS             complement(6170..7378)
                     /locus_tag="PLF85_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNE18705.1"
                     /translation="MSGFCCWQLFFVKASMKYLVPLLSLSASLACSGEAGMTVLQSFP
                     AVDAAFQGRIDLNNPLNYANQPVPGYIRKFNDTNFITNSGATLGRVLFYDKRLSSNET
                     IACASCHQQQHAFSDTAVASQGVNGTTGRHSMRLINARFALETRFFWNERAQSLEAQT
                     TMPVRDHGEMGFSGVNGDPEFSTLLTKLSAVDFYRELFKNAFGSEDITEARIQLALGQ
                     FIRSIQSFDSKFDAGRALVPNNNTPFANFTAEENLGKSLFLLPPVFSGSVRTGGGLGC
                     AGCHRAPEFDIDPNSANNGVIGTIAGGTDTTNTRSPSLRDLVKADGSLNGPMMHDGSM
                     TSLNAMLEHYNAISTSGNTNLDTRLRPNGSGQSLNMTAAEKNALAAFLKTLTGSAVYA
                     DQRWSNPFNK"
     gene            complement(7418..8575)
                     /locus_tag="PLF85_03260"
     CDS             complement(7418..8575)
                     /locus_tag="PLF85_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18706.1"
                     /translation="MHHSVLSRVKPRPEGGFAHDLKDRVLTGIGATADHFLRTFLSTR
                     VLVSNIPAIPKVMQADYRQKWEFYRNPEFITAPETFFAKPEWVEPVVRPADPMNLPIH
                     DAIYEDVIFPSEFEPLNPAYHASDVYPAKQAPAIARFIRHKQGIHGGKAEDRPTLIVV
                     HGYVLDGYKFNAQLFEVAKFFRLGFNILMYTMPYHGPRKAPGARFSGDGFMFFDVARI
                     AENVRWSVHDLTRYIDFLKKRSAAPVGMMGFSLGGYHTALMASLDKRLSFAIPVVPVI
                     TLFNVILQWQPSAAVIEGFLKVLKLDKNDINQLLFPVSPLARQPVIDHERLLIIAGTA
                     DRMAHPDHAHSLWHHWKHPKIYWFPGNHLVHFEKTHYMREVVAFLRGLKLF"
     gene            complement(8578..9456)
                     /locus_tag="PLF85_03265"
     CDS             complement(8578..9456)
                     /locus_tag="PLF85_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE18707.1"
                     /translation="MYPAIAGVEIEEKKIRTTDGFTLSVKCAGPADGQPILFLHGFPE
                     FWYGWRKQIGYFAMKGYRVIVPDQRGYNDSDKPERVCDYSIVQLAEDVAAIVRQLKLD
                     DCILVGHDWGAAAAWQAAIRHPSLFTRLVILNVPHPQVFMRTVFTNPKQFLKSWYMYF
                     FQLPVVPAWLASLDGYKRFADGMRSTANEGSFTDEDMLAYIDAWQKPDALDSMINWYR
                     AAFRHPEFNGPSDVIVPTLVLWGEGDRFLEASMAADSLHYCRTGRVKYFPRATHWLQH
                     DEPDAVNAEIEKFIKE"
     gene            complement(9509..10354)
                     /locus_tag="PLF85_03270"
     CDS             complement(9509..10354)
                     /locus_tag="PLF85_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18708.1"
                     /translation="MRKVAIILLVAALTGAGLLFYLIETRGLKTQFDAAVARAQNDTV
                     PVEGVVTAADIRHLPPLVQKYLTVTGAVGRSKIRNFRVVWDGALRSDAHALWMSAVMR
                     QENFFQDYSRDFYLTAFMKGLPVSVWHSYQKQRATMQVKIASLFPIVDIKGEELTTAE
                     TVTLFNDMCLLAPAALIDKRIRWRTVDTQQVEATFTNGKYSIKALLKFRESGELADFI
                     SDDRYYLTADNKLRRERWSTPVGEYREFAGRRVVSRGEAVWHLKEGSFAYGKFVLKNI
                     EYNVR"
     gene            complement(10347..10976)
                     /locus_tag="PLF85_03275"
     CDS             complement(10347..10976)
                     /locus_tag="PLF85_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNE18709.1"
                     /translation="MHQDMPELLLFDLGGVLIEVSTATLREIGGRDKSDVELWETWLT
                     CPVVEEYESGKIGNAEFAAGVLKTFRSEIPAEEFIHSFTAWPIGFYPGMTELLRKLRH
                     DFKLAYISNSNPLHYPRFQNEWQLDTYFDYHFASYQMGCVKPQRRIFEMVLDTLPFEA
                     KDIFFVDDNRLNVEAARELGIRSEIVRGPHELMSVLEKNGIVRNGGKHA"
     gene            11136..11615
                     /locus_tag="PLF85_03280"
     CDS             11136..11615
                     /locus_tag="PLF85_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18710.1"
                     /translation="MKKRIYSLMAVVPLLVFACKKEPQQPLEATTIDKVCTEAFGPFY
                     DERKYVRYHRVSFSGYLQTPKSAMVSDTMFVDVYEKPNRAGALVRSSFKIGSGKNRLE
                     RLKDRFKESDMKIESNSGVALGNGSKVTIQGAVSPGGVPGKWLDGCYVKVETVEAAQ"
     gene            complement(11619..12389)
                     /locus_tag="PLF85_03285"
     CDS             complement(11619..12389)
                     /locus_tag="PLF85_03285"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNE18711.1"
                     /translation="MCEILGMSANVPTDIVFSFTGLIRRGGETGPHKDGWGIAFYEDR
                     GLRLFHDPEESSRSKIAAFLQSYPIRSEVVIRHIRKANRGKVALKNTHPFVRELWGRN
                     WVFAHNGQLKGIKKVKFDSFEPVGTTDSEAAFCWLLGKLRKKFKAPPAQPALIKEIQK
                     LFSELAERGVANILMSDSNLLFTHCSNRLVHITRRAPFGKAQLLDADVTVDFQKATTE
                     KDVVTILATRPLTGNEDWQHYRSGEYRVWRRGESVFVG"
     gene            12508..12840
                     /gene="rpsP"
                     /locus_tag="PLF85_03290"
     CDS             12508..12840
                     /gene="rpsP"
                     /locus_tag="PLF85_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNE18712.1"
                     /translation="MTVKLRLQRYGTKKKPYYRIVAASSTVKRDGKFLDIIGLYHPLV
                     KEGEQIRLEKDKVNEWLKKGAQPSDTVKNILSKAGVWKPFAEAKALKDGAQQKKANEK
                     AKAKRAAK"
     gene            12853..13149
                     /locus_tag="PLF85_03295"
     CDS             12853..13149
                     /locus_tag="PLF85_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNE18713.1"
                     /translation="MTMTEQRNSDPASLVEYIARSLVRNPDDVQVLPVKSPASLILEL
                     RVNKDDIGMIIGKGGRIAKAVRVLLNAISVKKIILEDGREERYQKILLEIVDEA"
     gene            13206..13769
                     /locus_tag="PLF85_03300"
     CDS             13206..13769
                     /locus_tag="PLF85_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18714.1"
                     /translation="MGRPHGVRGMLHVDLAGNHLRDFVGQTVELCSAAQIGQVITSPK
                     ILKTLTLEKAEPLQGEVNRVAFAGLSDRDSAAQLTNLLIVQPFALMRQKARAQHGSQH
                     VPMANLWYFEMYGLSVVDSQSKIPIGIISQIEDLGKNTLITVTLNEAHSAQQREIILP
                     LEYPHWGEADLDRQEITLAEWQHFIEG"
     gene            13773..14432
                     /gene="trmD"
                     /locus_tag="PLF85_03305"
     CDS             13773..14432
                     /gene="trmD"
                     /locus_tag="PLF85_03305"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNE18715.1"
                     /translation="MQFNVVTLFPEFFASPLEASLLGKAVKSHTIGVKLVNPRDYTPY
                     KHKKVDDTPYGGGAGMVMMAEPIALAVEAIKSADADSRVILLSARGRLFDAQVAREYA
                     TQYKSLTLICGHYEGVDERVAEHIADESLRIGNYVLSGGEIAALTVIDAVARRQPGFM
                     GNADSLVHESFDTANQFEYPQYTRPDDWRGHKVPEVLLSGNHAKIAEWRKSESKKTLF
                     S"
BASE COUNT         3443 a         4037 c         3981 g         3057 t
ORIGIN      
        1 gagaatagcc gatgccctgc acaccgggta cactgtcatt gggacgcaat gactcgacgt
       61 accgcttcca gtcacggcgt gtgacacgat cagaagcgtc aaagagcccg gcagcgccgc
      121 gcaagatgag tgcataggcg ttcagacgct cgttgatctt gagctgaatc ttatctgcct
      181 ctgcgctgaa cccttgctgg cggtcgctat aagtttcgac accgacgatt gtggcggcga
      241 gaagggcaac taaagcgata ccgattgcgg taaccgcagg taacgcactg tatttcagga
      301 tgatacgggg aattctcaag acccgtaaaa atgcagggga acgacagggc gtcaatcggc
      361 catacgccgg tgcttacaca ggcgatagca gcccccaggg tcacgccacc gggcaacgta
      421 tataattggc tgttttttac ctgcgcggtt ttctcgccac gaactgcacg accgcgctct
      481 tgccattgtg gcccttgccc tcgtgaatct cgcgatcgag ttcttgtgcg atttcgacct
      541 cgagaccggc gagctcggtc ttcagcaggt tgcgggtcat gagcatggtc gcattgggtg
      601 gcccgccagt tttgtactgc agctgacgcg gagtgtacgc ctcgagaatg aagaagccgc
      661 ccggcttcag agccacaacc acccggcggt gtatttcggc gcgcagcggt tgcggcaggt
      721 ggcaccagac cgagacgatt gcatcatact ggcggtggcc catttcaaag tgcgcgaggt
      781 ccttgacgac ggtgcgtaca tgaactccgg tctgcattgc cagcttctgg agcttcacaa
      841 agccctgtgc tgaaccgtcg accgcggtaa cagaaaaacc ctgcttagcc agatagacgg
      901 cgttgcggcc ttcgccttca gcgaggcata acaccgaggc acccggcaat agtcggtgcg
      961 cctgggattt caaaaagtca ttgggctcgg tgccgtagaa gtactgctct gactggtacc
     1021 gttcatccca ttgctcgaag gctttgcctg cgctgttcat gctgtgacag ccaatgagcc
     1081 cgcaggcgcg taaatcaaaa tgcaatgggt atctggcaaa gggtggacac ggagtacacc
     1141 ctgcgtttcg ttgcctatgt cgtactccag cgagtttgcc gaccgcatcc gcaagattct
     1201 gtcgtcgcag acaactttcg tcgagaaaga aatgtttggt ggtgtcgctt ttatggtcga
     1261 cgacaagatg tgcgtcggca tcaaccagga caagagaaca ggggtagatc gcctcatggc
     1321 ccgcatcgac cctgagtttt attattcggc gctgaaacgc gaaggttgcc gcgaaatgga
     1381 tttcgccggg cggccgatga tcggttttgt tttcgttcac cctgaaggtt atgccaacga
     1441 agaagacctg gtcttctgga tcggcaaatc cctcgagtac aatcgtgtcg tgcctgtcag
     1501 cacggtgaaa aagaaacgtg ccttactggc tccgcctgaa aaaaaaattc gcaccgttaa
     1561 accactgcct aaatataaac cgccgaaagc caaaacggta aaaagtccag ccgctgcagg
     1621 caccaaaaaa gtcgtagcaa ataaaaagaa gtaaacccgt aaagaactgt taaaatataa
     1681 ctgaaggtaa tccccatgaa aattctgaag acactgctgg ttattatagt tatcctggct
     1741 ggcgctatca ctggtatcta tgcgtggctg ggtggtttca gtactgttgc agtaaaccgc
     1801 ggtgaaatcg gtccgtggga aattgtctat tcgactcata aggggcctta caagaacctg
     1861 aacaagagct ggacgaaatt tcagaaagac tgggaggccg cgggcctgaa agtatgcaat
     1921 agccttgccg tttatcttga tatgccaggc acaccagaag accagctgcg ctcggcgatt
     1981 gcctgtcgca tcgatgaact ggccccggca gaaaaggctg cgctgcgcgg caaactacct
     2041 gcgtttgtca tacccaaatc gcgcgcagtc atggcgactt tcccgtacaa gaaccctgct
     2101 tcgtatttcg tcgggcccat gaaggtgtat cctgcattcc acaaggtcat gacacagcag
     2161 aagctgctcg cccccgtggg tattgaaact tacggcgcga cgaacaaacc ggctgagcag
     2221 attggctacg ccatgccggt cgacagcaag cgcagcgact accaaaagct gatcgattcg
     2281 ttttgattct tgcggcagct atggaattcc atagctgccg gagttcggtt taaccgggta
     2341 attcccccat tttcatcctg ctttacaatg catcgataca tttagatgta tcgatctatg
     2401 cctctctatt ccgaaacaga aatccgccag gcgctggctg agcgtatcct gatactcgat
     2461 ggtgccatgg gcacgatgat ccagcgccgc agcctcaccg aagaggatta ccgcggcaca
     2521 ggcagcgtca gcccggtcga gttatggaaa tccatggccc ctgatttcca ggtgcctgcc
     2581 gaggtttgga attccatagc aaagagcgaa atacccctca agggtaactc tgacctgctc
     2641 tgcctgacgc ggcccgatgt cattgcggca attcaccgcg agtacatgga tgcaggtgcg
     2701 gacatcattg agaccaattc attcagcagt aacttcatct cgcaggccga ctataagctc
     2761 gaaccgctcg tctcggttct gaacgaggcg gccgtgcgct gtgttgcttt cgcgcgacag
     2821 gaatactata aaacaaacgg agaatcgaga aagatcttca ttgcgggttc gctcgggcca
     2881 accacgaaga ctgcgtcgat ttcgcctgat gtcaacaatc ccgcttaccg tgcaacgacc
     2941 ttcgacgaac taaaggcagc gttcaaggaa cagacagtcg ccctgctcag tgcaggctca
     3001 gacctgctgc taccagaaac gaatctcgac acactgaacg taaaagcagc gatcgtcgcc
     3061 ttcgaagaag cctttgaaga agtgggctac cgtgtgcccg tatcgatctc tgtaaccatt
     3121 acggacgcct ctggccgcac cctctcaggg caaactgccg aagcgttctt tacctcggtg
     3181 cgccatgcca acccgctgtc gataggtatc aactgcgcac ttggcgccaa agacatgaaa
     3241 ccgcacctgc aggctttgtc acgtgtctct gacgtgatga tttcgtgcta ccccaacgcg
     3301 ggattgccca acgctttcgg cggttacgac gagacaccgg ccatgttcgc ctctgacctt
     3361 gcagtcttcg cgaaggaagg cctgctcaat atcgccggcg gttgctgcgg cacaacaccc
     3421 gatcacatca aagagatggt aaagcgggtt gccaccgaaa agccgcgcgc ggtcgcagga
     3481 ctcgatgccc gcaaaggcct gagcctctcg ggtctcgaac cgctcaatgt cgacccttcg
     3541 actccgctca gggcaggcgg ctctcaattt ctcatgatcg gtgaacgcac aaacgtgacg
     3601 ggctcgccaa agtttaaaaa actgattctc gaagacaaat tcgacgaagc cctcgacgtt
     3661 gcgcggcagc aggtgatcgc gggcgccaac attatcgacg tcaacttcga tgaggctctg
     3721 ctcgacggcg aagcatgtat gacccgcttc ctgaacctga ttgcttcaga acccgacatc
     3781 gctcgcgtcc ccatcatgat cgactcatcg aagtggtcgg tcatctacgc gggtatgaag
     3841 tgcatccagg gcaagggtat cgtcaactcg atcagcctca aagaaggcga agagaagttt
     3901 ctcgaacagg cgcgcactgt gcacgaacac ggtttcgcca tggtagtcat ggcattcgac
     3961 gaacagggcc aggctgcgag cgaagatgac aaggtgcgca tctgcaagcg cgcttacaag
     4021 ctgctaacag agaaagcgca tgttcccccc tcagacatca tcttcgaccc caatattctc
     4081 acggtggcca ctggtatcga agagcacaac aactatgcgc tcgacttcat caacgcaaca
     4141 cgccgcatta agcaggaatg ccccggcgcc tacgtctcag gcggagtgag caacgtttca
     4201 ttttcattcc gcggcaacaa tgccgtgcgc gaggcgattc actcagtatt cctctaccac
     4261 gctattaagg ccggcctcga catgggtatt gtcaacgcgg gtatgctagc ggtctacgag
     4321 gacattcccc ccgagctgcg agaacacgtc gaagatgtga tcttgaaccg ccgccccgac
     4381 tcgacagaaa gactgctcgc gatcgccgac aagtacaagt cgggcggcaa agaacaggtc
     4441 aaagaagacc tctcgtggcg aaatggcccc ccagagcagc gcatcgaaca cgcgcttgtc
     4501 aagggcatcg acgcctacgt cgaagaagat gtcgaagagc tgcgccggaa ataccagccg
     4561 acgctgcgcg tgatcgaggg gccactgatg ggcggaatgc gcgtggtcgg cgaactcttc
     4621 ggtgcgggca agatgttcct gcctcaagtg gtgaagtccg cgcgcgtgat gaaaaaggcc
     4681 gtcgcgtacc tgctgccgtt catggaagcc gaaaaggcgg ctgcaggcac ttcgagtgca
     4741 cagaaaaaag tgctgatggc gacagtcaag ggcgacgtgc acgacatcgg taagaatatc
     4801 gtcggcgtcg tgctcgcgtg caacaactac gacgtgcacg atatgggagt catggtcccc
     4861 tgcgacaaga ttctcgaaaa agcgcgggag attaacgcag acgtgatcgg tctctcaggc
     4921 cttatcactc cctcgctgga tgaaatggtc tatgtcgcga gtgaaatgca gaaaggcggt
     4981 ttcacgcagc cgctgctgat cggcggcgca acgacttctg cggcacacac ggcagtgaag
     5041 attgtgccgg cgtacaacaa cgccgtggta cacgtacccg atgcttcgct tgttacgggg
     5101 gtgctcggta acctgctgag cgaagacctg aaaccggcgt atgtcagaca actcagcgca
     5161 gaacagacca aggtgcgcga agactacctg agcggaaaga aagagcgcca gttcctggca
     5221 ttcgccgatg cacgcgccca tgctccgcag tataagcctg cgccggtgaa gctaccgaac
     5281 aaactcggta taacgctgct cgacaacgta cgcctcggtg acctggtgcc gtacatcgac
     5341 tggtcaccct tcttcatggc atgggaactg cgtggccgtt acccgcgcat cctcgaagac
     5401 gaggttgtcg gcaaagaagc caccaaactc ttcaacgacg cacagaaaat actgacgcag
     5461 atactcaggg acaaaccctt taccccgcgc ggcgtcgtcg gtattttccc atgcaggcgt
     5521 gagggcgacg atgtgatcgt ctatgcggac gaaaatcgcg acaaggttct gcacaagctc
     5581 cactttttac gccagcagaa gaagaaggaa aacgaccaga tttactacag ccttgcagac
     5641 tttatcggtg aacaggatta tatcggtggt ttcgcggtca ctgccggcga cggcgcgggt
     5701 gaatacgccg cacgattcga aaaacagaac gacgattact ccgcaatcat ggtgaaggcg
     5761 ctcgccgaca ggttcgccga agcgttcgcg gaatatgcgc acgaaattgt gcgcaagcat
     5821 acctggggtt atgcagccga cgaaaaactc gacatcgaag gtctgatcaa agaagaatac
     5881 cagggtattc gccccgcacc aggctacccc gcgtcgcccg accacacgga aaagcgcacg
     5941 ctgttcgatt ggctgtcagt ggaaaagaat actggcattc agttgactga aagttttgcg
     6001 atgacgccgc catcgtcggt gagcgggctc tattttgcac atcccgaggc gaagtatttt
     6061 gcggtgggca agattgaccg cgaccagctc gaagactacg ccgcgcgtaa gggcaagccc
     6121 gtcgacgaga tggaacgctg gctgcggcct tacctggatt agcagactgc tatttattaa
     6181 acggattgct ccaccgctgg tcggcgtaaa ccgctgagcc ggtaagagtt ttcaggaagg
     6241 cagcgagggc gtttttctct gctgctgtca tgttcagcga ctgaccgctg ccattcggcc
     6301 gcaggcgggt atcgaggttg gtgttaccgc tggtgctgat ggcattgtag tgctcgagca
     6361 tggcattcag gctggtcatc gagccatcgt gcatcattgg cccgttgagg ctgccatcag
     6421 ctttcaccag gtcgcgaagg ctgggcgagc gggtgttcgt tgtgtcggta cctccggcga
     6481 tagtgccaat aacaccattg ttggcggagt tcggatcgat atcaaactcg ggagcgcggt
     6541 gacagcctgc acagccgaga ccaccaccgg tacgtaccga gccggaaaag accggcggta
     6601 gcaagaacag gctttttcca agattttctt cagcggtaaa attggcaaac ggggtattat
     6661 tgtttggcac aagcgcacgg ccggcatcaa actttgagtc aaaggactgg atgctacgga
     6721 taaattgccc caacgcaagc tgtattctag cctcggtaat gtcttcgctg ccaaatgcat
     6781 tcttgaatag ctcacggtag aagtcgactg cagaaagttt tgtgagcaag gtgctaaatt
     6841 ccgggtctcc attcacgccg ctgaacccca tctcgccgtg atcgcgcacc ggcatggtcg
     6901 tctgtgcttc aagactctgc gcgcgctcat tccagaagaa cctggtctcg agcgcgaaac
     6961 gtgcattaat caatcgcatc gagtggcggc ccgtggttcc atttaccccc tggctggcaa
     7021 ccgcggtatc agagaaagca tgctgctgct ggtggcagct ggcacacgca atggtttcgt
     7081 ttgaagataa ccgcttgtcg taaaagagca cgcgccccag cgtcgccccg ctgttggtaa
     7141 tgaagttcgt gtcgttgaat ttgcgaatgt agccgggcac tggctggttc gcgtagttga
     7201 gcgggttgtt cagatcaatc cgcccctgga atgctgcatc gactgcaggg aatgactgca
     7261 aaaccgtcat gccagcctca cccgaacagg caagactggc agacaatgac aggaggggta
     7321 caaggtattt catgctggcc ttcacgaaga agagctgcca gcagcagaaa ccggacatgc
     7381 attttccgct gccgtcaagg gcgccggaaa atgcgactca aaaaagtttt aatccccgca
     7441 agaaagccac gacctcgcgc atgtaatgcg tcttttcaaa gtgtaccagg tggttacccg
     7501 gaaaccaata gatcttcggg tgcttccagt ggtgccacag gctgtgcgcg tggtcggggt
     7561 gcgccatgcg gtcggctgta ccggcaatga tcagcaggcg ctcgtggtcg atcaccggtt
     7621 gccgcgcaag cggtgaaacg ggaaacaaca gctggttgat gtcattcttg tcgagcttca
     7681 ggaccttgag aaaaccttcg atcaccgcag cgctcggctg ccactgcagt atgacgttga
     7741 agagcgtgat caccggcacc acgggtatgg caaatgacag gcgcttatcg agcgatgcca
     7801 tcagcgccgt gtggtagcca ccgagtgaaa aacccatcat gcccacaggt gcggcggagc
     7861 gttttttgag aaagtcaatg tagcgcgtca ggtcatgcac cgaccagcgc acgttctcag
     7921 caatgcgcgc gacgtcgaaa aacatgaacc cgtcgccgct gaagcgcgca cccggcgcct
     7981 tgcgcgggcc gtggtacggc atcgtgtaca tgagaatatt gaaacccagg cgaaaaaact
     8041 tcgcgacttc aaaaagctgc gcgttgaact tgtagccatc gagcacatag ccgtgcacga
     8101 cgatcagcgt cggccggtct tccgctttac cgccatgaat gccttgtttg tgcctgataa
     8161 accgcgcaat cgccggcgcc tgctttgcgg ggtaaacgtc gcttgcatgg tatgccgggt
     8221 tgagtggctc aaactcgctg gggaaaatca catcttcata gatcgcatca tgtatgggca
     8281 ggttcatcgg atctgccggt cgaacgaccg gttcaaccca ttcgggtttg gcgaagaaag
     8341 tctctggtgc agtgataaat tccgggtttc gatagaactc ccacttctgc cggtagtcgg
     8401 cctgcatgac cttgggtatc gccggaatgt tgctcaccag tacgcgcgtc gaaagaaacg
     8461 tgcgcagaaa atggtcagcg gttgcgccga tgcccgtgag tacgcggtct ttgaggtcgt
     8521 gcgcgaaacc gccttcgggg cgcggcttga cgcgactgag aactgagtgg tgcataatta
     8581 ttccttaata aatttttcta tttcagcgtt cacggcatcg ggctcatcgt gctgcagcca
     8641 gtgtgtcgcg cgcgggaaat atttcacacg gcccgtgcgg caatagtgca ggctatccgc
     8701 cgccatcgag gcttcgagaa acctgtcgcc ttcgccccag agcaccaggg tcggcacaat
     8761 cacatcgctt ggcccgttga actcggggtg cctgaacgcc gcgcggtacc agttgatcat
     8821 cgaatcgagc gcgtcgggtt tctgccaggc atcgatgtac gcaagcatgt cttcatcggt
     8881 gaacgatcct tcattcgcgg tggagcgcat gccatcggcg aagcgcttgt agccgtcgag
     8941 cgatgcaagc catgccggta caacgggcag ctggaaaaaa tacatgtacc agctcttcag
     9001 aaactgcttc gggttcgtga agacagtccg cataaaaacc tgcgggtgtg gcacattgag
     9061 aatcaccagc cgcgtaaaca ggctcgggtg gcgaatcgcc gcctgccatg cagcagcggc
     9121 gccccagtcg tgcccaacaa gaatacaatc gtcaagcttg agctgccgca cgatcgctgc
     9181 cacatcttcg gcgagctgca cgatcgaata atcacagaca cgctctggct tatcgctgtc
     9241 attgtaaccg cgctggtcgg gcacaataac ccggtagccc ttcatggcaa aatagccgat
     9301 ctgcttgcgc cagccgtacc agaattcggg aaaaccgtgc aaaaaaagaa tcggttgccc
     9361 gtcagccggc cctgcacatt taacgctgag ggtgaagccg tcagtcgtgc ggattttctt
     9421 ttcttctatt tccactccgg cgattgcggg gtacataaga cctctgacac cggctcagac
     9481 aacgcggaaa gaagtttgcc gcttctgcct agcgcacatt gtactcaatg ttcttgagta
     9541 caaacttacc atatgcgaac gaaccttctt tcaggtgcca gactgcctcg ccgcggctga
     9601 ctacccgcct gccggcaaat tcgcggtatt cacctaccgg cgtagaccag cgttcgcggc
     9661 ggagcttgtt atctgctgtc aggtagtacc tgtcgtcaga gataaaatct gcgagctcgc
     9721 cgctttccct gaatttgagc agcgctttta tgctgtattt gccgtttgtg aaagtcgctt
     9781 caacctgctg cgtgtcgact gtacgccatc tgatgcgctt atcgatcagc gctgcgggcg
     9841 cgagcaggca catgtcattg aacagggtca ccgtctctgc ggtagtgagt tcttctccct
     9901 ttatatcgac gatcgggaac agcgaagcaa tctttacctg catcgtcgcg cgctgtttct
     9961 ggtacgaatg ccagaccgaa accggcagac ctttcatgaa agcggtcagg taaaagtcac
    10021 gcgagtaatc ctggaaaaag ttttcctgcc gcatcacggc cgacatccag agcgcgtggg
    10081 cgtcgctgcg cagcgcgcca tcccagacga cgcggaagtt gcgtatttta gagcgcccga
    10141 ctgcgccggt cacggtcagg tacttttgca ccagtggcgg caaatgcctg atgtcggccg
    10201 cagtgacgac accttcaaca ggtactgtat cgttctgcgc acgcgccact gctgcatcga
    10261 actgcgtttt cagcccgcgc gtctcgatca ggtaaaaaag cagacctgcg ccggtgagcg
    10321 cagccacaag caggataatt gccactttac gcatgttttc ccccgttgcg cacaatacca
    10381 tttttttcca gcacgctcat gagctcgtgc gggccgcgta caatttctga gcgaataccc
    10441 aactcgcgtg ctgcttcaac gttgagccgg ttgtcatcga caaaaaaaat gtcctttgct
    10501 tcaaacggca gtgtatcgag caccatttcg aatatcctgc gctgcggctt gacacaaccc
    10561 atctggtatg aagcgaagtg gtagtcaaaa taggtatcga gctgccattc attctggaag
    10621 cgcgggtaat gcagcgggtt ggagttcgaa atatacgcca gcttgaagtc gtgccgcagt
    10681 ttgcgcagaa gctccgtcat gccggggtag aagccgatag gccaggcagt aaacgagtga
    10741 atgaattctt ctgccggtat ctccgagcga aaggtcttca gtacacctgc agcaaactca
    10801 gcattaccaa tcttacccga ttcgtactct tcgaccaccg ggcaggtgag ccaggtttcc
    10861 cagagttcga cgtcgctctt gtcgcggccg ccgatctcgc gcagcgtcgc ggtgctcacc
    10921 tcaatgagca ctccgcctaa atcaaagagc agtagttctg gcatatcttg atgcatctga
    10981 aatatggact tttttcgtaa gtctgagcgt aaacgagttt gcgggcataa gaaaacaggc
    11041 ccaattactg caacgacaag taggatgatc aaccgagcga ggggtttcgc cattcggtat
    11101 tgacgcatca cagttgtatt ccaaggtttg cctctatgaa aaaaaggata tactccctta
    11161 tggccgtcgt gccactcttg gtctttgcct gcaagaaaga accgcagcaa ccgcttgagg
    11221 caacgactat cgataaagtc tgtaccgagg cattcggtcc attttacgat gaacgaaagt
    11281 acgtacgcta ccaccgcgtc tcgttcagtg gttacctgca aacaccgaag agtgccatgg
    11341 tcagcgacac gatgttcgtc gacgtgtacg aaaaacccaa tcgtgcaggt gcccttgtcc
    11401 gctcttcttt caaaattgga tcaggcaaaa ataggctcga aaggctcaaa gatcgtttca
    11461 aagaatcaga tatgaaaatt gaaagcaaca gtggcgtagc gctcggcaac ggttcaaaag
    11521 taaccatcca gggtgcagtg tcaccaggcg gcgtgcccgg caagtggctc gatggctgct
    11581 acgtcaaagt cgaaacagtg gaagctgcgc agtagtagct aacctacaaa cacactctct
    11641 cccctgcgcc agacgcggta ttcgcctgac cggtaatgtt gccagtcttc attgccggtc
    11701 agtggccgcg tcgcgagaat cgtcacgaca tctttctcgg tcgttgcttt ctggaaatcg
    11761 actgtcacat cggcatcgag cagctgcgcc ttgccgaacg gcgcacgccg cgtgatatgc
    11821 acgaggcggt tgctgcagtg cgtaaacagc aggtttgaat cgctcatcaa tatattcgcc
    11881 acgccccgtt ctgcgagctc tgaaaagagc ttctgtattt ctttgatgag cgccggttgc
    11941 gccggcggag ctttgaattt ttttcgcaat ttgccgagca accagcagaa cgccgcttct
    12001 gaatcggtgg tgccgacagg ttcgaagctg tcgaatttta ccttcttgat acctttaagc
    12061 tggccgttat gcgcgaacac ccagttgcgc ccccataact cgcgcacgaa cgggtgcgtg
    12121 ttcttgagcg ccaccttgcc gcggttggcc ttgcgtatat ggcgaatcac cacttcactg
    12181 cgaatcgggt aagactgcaa gaacgcggcg atcttcgaac ggctgctctc ttcggggtcg
    12241 tggaacaacc gcagtcccct gtcttcatag aaggcgatgc cccagccgtc cttatgcggc
    12301 ccggtctcgc caccccggcg gattaacccc gtgaaactga atacaatgtc ggtggggaca
    12361 ttggcgctca tacccagaat ttcgcacata ggccggggcc actcctgtag atgtgacata
    12421 atacgtacaa tatgttcgtt gagccactgc gcggctcaac gaacatatta aatttgacgg
    12481 cctgtctcaa aggccacttt tacccgcatg actgttaaac ttcgcctgca gcgctacggc
    12541 acaaagaaga agccgtacta ccgcattgtc gctgcatctt cgaccgtcaa acgcgacggt
    12601 aaattcctgg atatcatcgg cctttaccac ccgctcgtaa aagagggaga acaaattcgc
    12661 ctcgaaaaag acaaggtaaa tgaatggctc aaaaaaggtg cacaaccctc tgacacagta
    12721 aaaaacatcc tgtcaaaagc tggcgtatgg aagccgtttg cagaagctaa agccctgaag
    12781 gacggcgcgc agcagaagaa agccaacgaa aaagcgaaag caaaacgcgc tgcgaaataa
    12841 attcgagcgt agatgaccat gaccgaacag cgcaattcag atcccgcttc actcgttgag
    12901 tacattgcgc gcagcctcgt gcgcaatccc gacgatgtgc aggtactccc ggtaaaaagc
    12961 ccggcttccc tgattctcga actgcgtgtg aacaaagacg acatcggtat gatcatcggc
    13021 aaaggcggcc gcattgcgaa ggcggtgcgt gtgctgctca atgctatttc agtgaaaaag
    13081 attattctcg aagacggccg cgaagagcgt taccagaaaa tcctcctcga aatcgtcgac
    13141 gaggcgtgat acagccccct gctaaccaca tcgaccccca aaattcttat gtagtgctcg
    13201 cccgcgtggg caggccgcac ggcgttcgtg gcatgctgca cgtcgacctg gcaggcaacc
    13261 acctgcgcga ctttgtcggc caaaccgtag agctttgcag cgcggcgcag attggccagg
    13321 tgattacgtc tcccaaaatt ctgaaaacgc tcacgctgga aaaagcagag cctttgcagg
    13381 gcgaggttaa ccgcgtcgcc ttcgccgggc tcagcgaccg cgacagcgca gcgcagctca
    13441 ccaacctgct catcgtgcag cctttcgctc tgatgcggca gaaggcgcgc gcacagcacg
    13501 gcagccagca tgtacccatg gccaacctgt ggtatttcga aatgtacggc ctcagcgtcg
    13561 tcgacagcca gagcaaaatt cctatcggta tcatcagcca gatcgaagat ttgggcaaaa
    13621 acaccctgat cacagttacc ctgaacgagg cacactccgc tcagcagcgg gaaattatac
    13681 tgccgcttga atacccgcat tggggtgagg cggatttaga ccggcaggag ataacgcttg
    13741 ccgagtggca gcatttcatc gaaggttgag ccgtgcagtt caacgtcgtg actttgttcc
    13801 ccgaattttt tgccagcccg cttgaagcgt ctctgttggg taaagccgtg aagtcgcaca
    13861 ccatcggggt aaaactcgtg aacccgcgcg attacacgcc gtacaagcac aagaaggtcg
    13921 acgacacgcc ctacggcggc ggcgccggca tggtgatgat ggcagagccg attgcgctcg
    13981 cggttgaggc aatcaaatcg gctgacgcag acagccgcgt gattctgctc tctgcccgcg
    14041 gcaggctgtt tgacgcgcag gttgcgcgcg aatatgccac gcaatacaaa agcctcaccc
    14101 tcatttgcgg gcactacgaa ggtgtcgatg agcgcgtggc agagcacatt gctgatgaaa
    14161 gcctgcgcat cggcaactac gtcctttcag gaggtgaaat tgcagcgctc acggtgattg
    14221 acgccgtcgc acgccgccag ccaggcttta tgggcaacgc agattcgctc gtacacgaat
    14281 cgttcgatac ggccaaccag tttgaatatc cgcagtacac ccgccccgac gactggcgcg
    14341 gtcacaaggt acccgaagtg ctgctgtcag gcaaccatgc caaaatcgcc gagtggagaa
    14401 aaagcgaatc gaagaaaact cttttcagct gaggctacgc ctcagctgaa aagagaattg
    14461 atgccaaatt tgtcgcgtgc gatggcgaca aacaggttga gatagataat ctgcagaa
//
LOCUS       DAOQJF010000035        14364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2035_length_14364_cov_5.207142, whole genome shotgun sequence.
ACCESSION   DAOQJF010000035 DAOQJF010000000
VERSION     DAOQJF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14364
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2035_length_14364_cov_5.207142"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..582)
                     /locus_tag="PLF85_03310"
     CDS             complement(34..582)
                     /locus_tag="PLF85_03310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE18716.1"
                     /translation="MNRITDYRLILLAATACALLAGACRPAEKLLNDAIIFPELSGQG
                     QFQLGAVEPRIQKVVVNFFAPDCPPCEKEIPALKQFYQAHSGDRDLLFVSIGSSLKAV
                     GTADPSKAPQDAEIRAEISNFIRKFAVDYQQYVAGGAELAAWRVTGFPETFVFVREGI
                     TWKLKRKFISEITRENLENELK"
     gene            complement(597..1394)
                     /locus_tag="PLF85_03315"
     CDS             complement(597..1394)
                     /locus_tag="PLF85_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE18717.1"
                     /translation="MEAKGKIVWITGASSGIGRALAAEFAGLGANLVLSARNETALQD
                     FAREQRLPPERVLILPVDLQQYKKLPAAVNKVLKKFGSIDILVNNGGISQRSRAHETD
                     MAVYEQLMAVNYFGNIALTLGVLPHMRARRQGQIVTVSSVAGKFGTPLRSGYSASKFA
                     LSGFYEALRAENWRENIRVTVVLPGFIRTNVSANALTAGGKPQGKMDVGQAAGMSAEE
                     CARIMARGILKGKNEIYVGGIKERFALHLSRHLPGVFARIIRKAKVT"
     gene            complement(1395..3203)
                     /locus_tag="PLF85_03320"
     CDS             complement(1395..3203)
                     /locus_tag="PLF85_03320"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNE18718.1"
                     /translation="MKAVFDFFRARVALLLIGVTLLLYVASGFSEKGFGYFIVLFVAG
                     LLYTLCGVSLYTLFVAGERRGGAFRLMELAYWLMLFSAVCYFVQVDAVWLKITPESRG
                     QLIERLRQVLQIFYFSGFAFVLIALAASVGVKLEAKEAPAAMATLGGLLLLLVAVNYW
                     AHITPASVDMTLMRRFSLSTDSRELLRGIEGKVKVTAFFPFFSELYRDVELLLRDAVS
                     ANGKISYTFIDPLREKNLADEKKVDRIGTILIEAEDPQESDAAKKLRTSRFEILDEEG
                     LKRLERELVSNLLQVSGKKRNIYYTQGHEEKSVSGNFKDDTISLFDENLRALRHHLKQ
                     LTPAEGFPAKMPQADLLLIIGPQRDFSAVEKKTLKKYFDDGGKILLALDPESSADFSF
                     LLEPLKVKYARQKLHSDYALPPGKTTLQSVNYSEHAITAPFVKRPEERKITIFPGAGY
                     FETGTESNPDFDVNYFLLSHFTSWVDKVPNGLRDDKVEPVASFKIGLAALSKKNGARL
                     VAVTDSDFLVNRFADMQQNRELAMRLVGWLAEEDKLAGIVAGKYDDEKVKLTGTKDTV
                     VFHLFLYIYPATLLLAGFMIVRAKRRRMSEHTRLQV"
     gene            complement(3205..3936)
                     /locus_tag="PLF85_03325"
     CDS             complement(3205..3936)
                     /locus_tag="PLF85_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE18719.1"
                     /translation="MKRSALTVVWQICRRELSSYYQSALAWSILSISFSLAFLWGYAY
                     IRHESTSFLALQRIFYMLSGTTMITGVLISMRLIAEEKMQGTIELLFTSPINEAQLVL
                     GKFSSAVVFLLVQAVVTLPIPLLVVVYGGSNLGHVLAGMVGVLLIGSATAAMGTFYSA
                     LTKIQLLAALMTAANLVFFLLLGFFSPFIDQPLKSVLREFSFYVHFMDFEKGVLSLKH
                     IVFYLSIVAFYLYAASLALAARRWR"
     gene            complement(3933..5636)
                     /locus_tag="PLF85_03330"
     CDS             complement(3933..5636)
                     /locus_tag="PLF85_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE18720.1"
                     /translation="MATRKVLFQDSNVSMADVFKTDTAVADIRSGAIVSGKIVGITPE
                     FAIIDMGGKSEGRIGIREFDVEPAVGETVEALVRSHDQETGLVQLSKRELELRRGWEI
                     VKEAFEKQVPVNALVKRSMREGYMVNVEGVFMFLPHTQVGLLGPRERGAKKINIVGQT
                     YLVKILDLNDRRKTGTVSRKVFQDEQNATVWKDIGDKIKIGHIIKGIVTKHTNSGVFI
                     NVEGIEGFLHRSNITWERKADQAKDKLEIGSEVNVRVLEIEPENHRLSLGLKQLTDDP
                     WSTVLTRFKVGDKVTGKVSFVANYGAFVELGEGLEGLLHITEMSWTRKVNHANEIVKV
                     GQEIETKIIGINKDDKRISLGLRQLHANPWDTIRTEVKVGETIKGKIRGLTNNGIFVA
                     ITDSIDGLIRKEDIGWTEPAPDPKRTYKIGDEIEFKVIEINSEERKVQCSIRHTLPNP
                     YKKLRDKYPRGTVIEGAVSGVVDFGIFIKFDEGYEGLAHISTMEKEQSTNFKKTFAKG
                     QPVKAVIKSIDPESRKISLSLKDVTYALEKIEMRQYLEKENSNKTETASPFANLKKLV
                     S"
     gene            complement(5671..7719)
                     /gene="aroA"
                     /locus_tag="PLF85_03335"
     CDS             complement(5671..7719)
                     /gene="aroA"
                     /locus_tag="PLF85_03335"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNE18721.1"
                     /translation="MPMRGTLTFPGDKSLSHRAAIFAALAEGASTIHNFLDAGDTMNT
                     LHAMQALGAKVTDLGNRSFRVESPGLKGLKSPAGVIDCGNSGTGSRLIMGLIAGLEGV
                     TAVIDGDASLRKRPMRRVTGPLEKVGAKFEWLGEKDCLPVRVTGTRLPPIEFTETLGS
                     AQVKSAVMLAAIASQTSLVLDEPIPSRDHTENMLRHAGAVVKKERSGEGFRLTLDAGV
                     TIQPREYRIWGDVSSAAFFAVGASLLPGSEVLIRHVLLNPYRDRYLQALRDMGAAIEV
                     LPQRDECGEKGGDVLVKHARLHGVAIPPESIPALIDELPVLTIAGTFAAGKFSYRNAK
                     ELRVKESDRISIMVRNLAQSHVHVEEFEDGLSLDGDPGRPLTGTIEPDMDHRIVMSFE
                     IADLISRHIRNPHAAETEVAPALQIQGREWVATSFPSFYEKLQATLEFSPPAAASHVQ
                     PQVITVDGPAGSGKSTVAYTVAKSLGYNQVDSGALYRAFTWAGYREYQRLGLSTEWAN
                     AVKEDKALSAYLAALPLEIRFGTDGRQHVLVDGVEVGEELRSVEVAQRIKPVADARYL
                     RERVREVLVAAAQRYSLVADGRDMGTEVFTQAPFKFFLTADSRIRAERRLAEFREKNP
                     TISLDEVQHQIEARDKDDASREFGGLRPAAEAIFIDTSSHTQEQVADLMLAYIRNSRL
                     "
     gene            7879..8820
                     /locus_tag="PLF85_03340"
     CDS             7879..8820
                     /locus_tag="PLF85_03340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNE18722.1"
                     /translation="MVEAELKVLAGRSNLELAQEIGDELGIKVGGVECKRFSDGEISV
                     KISENVRGSDVFVIQSTSNPANDNLMELMLIVDALRRASARRITAVIPYYGYARQDRK
                     VEPRVPISAKVVATMLEAIGVKRVLSMDLHANQIQGFFEIPVDHLFGAPVLVKYFKEL
                     NLSDLVIVSPDSGGAERARFFGKQLGANMAIIDKRRERANEAEVMHIVGNVEGKNCLL
                     IDDMIDTAGTICKAAIALREKGALKIIAAATHGVLSGNAIQNLISSPIDEVVVTNTLQ
                     ISDEKRFPRMRVLSVASLFATAIKRIHEEQSVSSLFL"
     gene            8837..9469
                     /locus_tag="PLF85_03345"
     CDS             8837..9469
                     /locus_tag="PLF85_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNE18723.1"
                     /translation="MEKATLKAQKRDGKVGGKNLLRAGIIPAVIYNHGKSESIQVLEK
                     DTRKLFAHGISESRLIDLDIDGKIEQVFVKDYQSHPVNEMLQHLDFYRVSQDEKLRTR
                     IAVHVEGKAEGVKNGGVLEVFLTEVPYETYPRFLTDFLTIDISHLNIGDSIHVSDVKL
                     PQDSRILMDAQTVICHVTHAAKEEAAAPAAGAAPAAAAAPAAAKAEPAKK"
     gene            9498..10127
                     /gene="pth"
                     /locus_tag="PLF85_03350"
     CDS             9498..10127
                     /gene="pth"
                     /locus_tag="PLF85_03350"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNE18724.1"
                     /translation="MALSNLKLVIGLGNPGDRYMNNRKNIGFKVVDVLANNTGIHIKT
                     KKKKSLLGQGDFEGHDIVLLKPQTFMDLSGEAVLYIASFLRVQVQNIIVIHDDITLNY
                     GDVVVAVGPPEIPNIGVESIARGLKSDKFVRIRMGVGPVKIRGKEFPNPKENEIKAFM
                     LSDFTLEENMKIIEVINDAEAALRSILTQDIKDVLARFKLGAKFSKNTA"
     gene            10136..11275
                     /locus_tag="PLF85_03355"
     CDS             10136..11275
                     /locus_tag="PLF85_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE18725.1"
                     /translation="MSLRQALATFASVAHKPHWLIAFSAGADSVLLLDLAVEAALRSA
                     DIRVTAFYLHHYSTPVESERRRAISFMASRARQLLGDRFAFCELSSDITMRAHRLRRS
                     WEHTASLVRRRHLERLARRLGGAQVFTGHQLSDYRETLQLRAERCVPRSAWPALSVHD
                     SNTDFLRPLAFFTREQVRAFARDRGLSWFEDPSNSDTSIARNRLRATEQPGENILVPP
                     EHCTDTSQPKPRRVHPREMRMPETEWRNLAPVMRARAVYQAWQQLFIVKRFTRNDFAR
                     AQRLPFSLRPFFAHAENFPEGEFIVFRRGLGSMSLNSRATQNSMRGDAITRAVKLRQP
                     YGHKAVTKIFSERRLSPRQRRLTWLEMADSPNEALRIFFPDGAVL"
     gene            11272..12327
                     /locus_tag="PLF85_03360"
     CDS             11272..12327
                     /locus_tag="PLF85_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE18726.1"
                     /translation="MKFRRVVAPGKTLVVAGIDRRFTVFTAWFLHLWQQRFSTRPFLA
                     AATRPGDLGAPLLNSLAEPVELPGSFFPLLWFLIRGRFSTLIILDPDLRATLKIRIAA
                     RLAGIRHRAGFAPLRRITGLNLSLPFNNENHHYVHQLRTFFEYITGEKTSGWSTPDFP
                     LAASSGLADVPAEPFGIIAYNPDETESEHLESQLKKLTNLMARASHCVLILRSRHLGR
                     QALTRFARSFSEIMTEQALANTTLLVLPTEETLVGLLREAAWIAAADTELLNLAALFA
                     VPSVAVFGPLNERVWQPFSVRTRALTGEFDCRPCTAYPGRVECSNSVPWACLAGASAE
                     LMAATLTAVLRRKRHVT"
     gene            12399..13706
                     /locus_tag="PLF85_03365"
     CDS             12399..13706
                     /locus_tag="PLF85_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE18727.1"
                     /translation="MRLIQQMDFRFFPALLLLVVALPLSAAPGKPGARAAGRADKKPA
                     VDLFAEAKDKVLRWQFAEGDILELKKFSDQVVRTGASSVKRSVFHRVLLEARTPDPAK
                     GFPLEGTFTTLIKSGETKNVYAEAEKYTASFVLQPAGQFVADADQYMPNIRSVPSFSE
                     SRDPALKEEAGMEPGTTWEKPGAEVMRFDKLVNVPFNVRYEYRGLENVKSEEGEKSCH
                     KFISNYELNYGDNNGTGPRVFGYVTAIWFWDAAQGIPYYAQEDYNVIIVNEQGLANEF
                     KIKSRSYYRKFRARGDEAKVALAQKIKEDLVKDNPQLDVRVTDHGVAIALPDVFFATD
                     SAKLSSDAKKVIERVGKTLRSLQNRHIRVRGHTDSTGDEKYNQQLSEKRAESVADYLI
                     DEAELNPDSVSYDGRGARDPVADNSLEEGRARNRRVEIILLDR"
     gene            complement(13708..>14364)
                     /locus_tag="PLF85_03370"
     CDS             complement(13708..>14364)
                     /locus_tag="PLF85_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18728.1"
                     /translation="NTLVYAEFAGKRSLRRINSSGVSDGTEIARSDRTGELLLLDDGS
                     VALVAQNLAYSGADGKNIGRIAKSRGWRSFTVVPAGTRYLAGDFNKLSYRKGVALWGE
                     DAEGKTTWTKNFAGEAAMTIEQMTANSARACVAGKTDGALHGQAHSNPGKMTDYYVLC
                     TSPSGVRLYTRVLPADGLKLASMRLNGQGDLVLAFSGGDRKNPDIIFARIDRNGNVFR
                     "
BASE COUNT         3219 a         4150 c         3884 g         3111 t
ORIGIN      
        1 ccggccgcag acagccggtg aaattttgaa tgatcacttg agctcatttt cgaggttctc
       61 ccgtgtgatt tcgctgatga atttccgctt caacttccac gtgattcctt cgcgcacaaa
      121 gacaaaggtt tcaggaaaac ctgtaacgcg ccacgctgcg agttcagcgc cgcccgcgac
      181 gtattgttgg tagtcgacgg caaacttgcg gataaaatta ctgatctcag ccctgatctc
      241 tgcatcctgc ggagcctttg acggatcggc agttcccacg gccttgagcg aagagccgat
      301 cgaaacaaaa agtaaatccc tgtcgcctga gtgcgcctgg tagaactgct tgagcgccgg
      361 aatctctttt tcacagggtg ggcaatccgg cgcaaaaaaa ttcaccacaa ccttttgaat
      421 ccgcggttcg actgcaccta gctggaactg cccctgcccc gaaagctcgg gaaaaataat
      481 cgcatcgttc aggagctttt cagccggtcg gcatgcacct gccaacagcg cgcaggctgt
      541 agccgccagc agtattagcc tgtaatctgt tattctattc atggattgca tacgcatcat
      601 gtaacctttg ccttgcgaat gatgcgcgca aagacaccgg gcaaatgccg gctcaggtgc
      661 agcgcgaacc gctcttttat tccgcctacg taaatttcgt ttttgccttt caatatgccc
      721 ctggccataa tgcgggcaca ctcctcagct gacatgccag ccgcctgccc gacatccatc
      781 ttaccctgtg gcttgccacc ggcagtcagt gcattcgccg acacgttcgt cctgataaat
      841 cccggcagaa cgaccgtcac ccgaatattt tcccgccagt tttctgcccg cagtgcttcg
      901 tagaaacccg acagcgcaaa cttgctggcc gaataccccg agcgcagcgg agtgccaaac
      961 ttgccggcaa cactggagac cgtaacgatc tgtccctgcc tccgggcccg catatgcggc
     1021 aacacgccca gcgtgagggc aatattccca aaatagttca cagccataag ctgctcgtag
     1081 acagccatgt cagtctcgtg cgcgcgggag cgctggctga ttccgccatt attaaccagg
     1141 atgtcgatgg agccgaattt cttcagaacc ttgttcaccg cggctggaag tttcttgtac
     1201 tgctgcaggt ctacaggcaa gataagtaca cgctcaggcg gcaggcgctg ttccctggca
     1261 aaatcctgca gggccgtttc gttacgggca gatagaacca ggtttgctcc cagtccggcg
     1321 aattctgctg cgagggcacg tccgattccc gagcttgcac cggtgatcca gactattttg
     1381 ccctttgctt ccatttagac ctgcaatctc gtgtgttcag acatgcgccg gcgttttgcc
     1441 cggacaatca taaacccggc gagcaaaagc gttgcggggt aaatatacag gaaaagatgg
     1501 aagacaacgg tatctttggt tcccgtaagc ttcactttct catcgtcata ttttcccgcg
     1561 acaatacccg ccagcttgtc ctcttcggcc aaccagccga cgaggcgcat ggcgagttca
     1621 cggttctgct gcatatcggc aaagcggttc acgagaaaat ctgaatcggt gacggcgacg
     1681 agccgcgcac cgttcttttt cgacaacgct gccaacccaa ttttgaaaga agcgacaggt
     1741 tcaactttat cgtcgcgcag accattgggt accttgtcta cccagctggt aaaatgactc
     1801 agcagaaagt agttcacatc aaaatcggga ttactctcgg taccagtttc aaaataaccc
     1861 gcaccgggaa atatggtaat cttacgttct tcgggacgct tcacgaaagg tgcggtaatc
     1921 gcatgttcag agtagttgac cgactgcaag gtcgtcttgc ccggtggcaa cgcgtaatcg
     1981 ctgtgcagct tctgccgcgc atacttcacc ttgagaggtt cgagcagaaa agaaaagtca
     2041 gccgatgatt ccgggtcgag ggcgagcaga atttttccgc cgtcgtcgaa gtattttttc
     2101 agggtcttct tctcgacagc gctgaaatca cgctgtggcc cgataatcag caacaggtct
     2161 gcctgcggca tctttgccgg gaaaccttcg gcaggggtca gttgcttcag gtggtggcgc
     2221 agagcccgca ggttttcgtc gaacaggctg atggtgtcgt ccttaaaatt gccactgacc
     2281 gacttctctt cgtggccctg cgtatagtag atattgcgct tcttgccgct gacctgcagc
     2341 agattgctca cgagctcacg ctcgagacgc ttcaggccct cttcatcaag aatttcaaac
     2401 cggctggtgc gcagcttctt tgcagcgtct gattcctgcg gatcttctgc ttcgatcagg
     2461 atcgtgccga tgcggtcgac tttcttctcg tcggcaagat tcttttcgcg caggggatcg
     2521 atgaaagtat atgaaatctt gccgttcgct gacacagcgt cgcgcagcag cagctcaaca
     2581 tcgcggtaaa gctcgctgaa aaaaggaaag aatgcggtga ccttgacttt gccctcgatt
     2641 ccgcgcagca gctcgcggct gtcggtagaa agggagaatc tccgcatcag cgtcatgtcc
     2701 accgaggccg gggtaatatg cgcccagtaa ttgactgcaa cgagcaacag cagcaatccg
     2761 ccgagcgttg ccatagccgc aggcgcctct ttggcttcaa gctttacgcc caccgacgcg
     2821 gccagcgcga tgaggacaaa agcaaaaccc gaaaaataga atatctgcag gacctggcgc
     2881 aagcgctcga ttagctgccc gcggctctcg ggagtaatct tcagccagac ggcatcaacc
     2941 tgcacaaaat aacatactgc tgaaaagagc atcaaccagt aagcgagctc cataaggcga
     3001 aacgctccgc cccgcctctc acctgccacg aagagcgtgt agagcgaaac cccgcagagg
     3061 gtgtaaagca gcccggcgac aaacagaaca atgaagtagc cgaagccctt ctctgaaaaa
     3121 ccagatgcca catagagcaa cagggtgacg ccgatcagca gcaacgcgac gcgggcgcga
     3181 aagaagtcaa acacagcttt catatcaacg ccaccttctt gctgccagcg cgagtgaagc
     3241 agcgtagaga tagaatgcca cgatactcag gtagaagaca atgtgcttga gcgagaggac
     3301 tcccttttcg aaatccatga agtgcacgta gaagctgaac tcgcgtaaca cgctcttcag
     3361 tggttggtcg ataaaaggag aaaagaagcc gagcaacagg aaaaaaacga gattcgccgc
     3421 agtcatcagc gccgccagca gctgtatttt tgtcagtgcc gagtaaaaag tccccatcgc
     3481 ggccgttgcc gaaccgatca gcaggactcc caccatgcct gcaagcacgt gaccaaggtt
     3541 actgccgcca tacacaacaa caagcagggg aatcggcagc gtgacgaccg cctgtaccag
     3601 gaggaaaact accgccgaag aaaacttacc gagtaccagc tgcgcttcgt taatgggcga
     3661 ggtgaaaagc agctcaatgg taccctgcat tttttcttct gcaatcagcc gcatcgagat
     3721 aagaaccccg gtgatcattg tcgtaccgga gagcatgtag aatatacgct gcagcgccag
     3781 aaacgaagtg ctctcgtgcc tgatgtaggc atagccccac aggaatgcga ggctaaatga
     3841 aatactcaag atagaccagg caagggcaga ctggtagtag ctgctgagct cacgccggca
     3901 aatctgccag acgacggtca acgcagaacg tttcacgaca cgagtttttt caggttggca
     3961 aacgggctcg cggtctcagt cttattgctg ttctctttct cgagatactg gcgcatttcg
     4021 attttttcga gagcgtaagt tacatctttg agcgacagcg atatcttgcg actttccgga
     4081 tcaatgctct tgatcacagc tttcacgggc tgacctttcg cgaatgtctt cttgaagttg
     4141 gtcgactgct ctttttccat cgttgagatg tgagcgaggc cttcataacc ttcgtcgaat
     4201 ttgatgaaga taccaaaatc taccacaccc gatactgcac cttcgatcac ggtaccgcgc
     4261 gggtacttgt cgcgcagctt cttataaggg ttgggcagcg tgtggcggat agagcactgt
     4321 actttgcgct cttcagaatt gatttcaata actttgaact caatctcgtc gccgatcttg
     4381 taagttcttt ttgggtccgg ggctggctct gtccagccga tgtcttcttt gcggatcagg
     4441 ccatcgatgc tatcggtgat tgcgacaaag ataccattgt ttgtcaggcc gcgtatcttg
     4501 cctttgatgg tttcgccaac cttcacttca gtgcgaatgg tatcccatgg gttagcatgc
     4561 agctggcgta ggccgagcga tatgcgctta tcgtctttat tgatgccgat gatcttggtt
     4621 tcgatctcct gccctacttt gacgatttcg ttcgcatggt tcaccttgcg ggtccagctc
     4681 atttcggtga tgtgcagcag gccttcgagg ccttcgccga gctcaacaaa cgctccgtag
     4741 ttggcaacga aagagacctt tccggtcact ttgtcgccta ctttgaagcg cgtgagcact
     4801 gtcgaccatg gatcgtcagt gagctgcttc aggccgagcg agaggcggtg gttttcgggc
     4861 tcgatctcga gcacgcgcac attgacttcg ctgccaattt cgagcttgtc tttcgcctgg
     4921 tcggcttttc tttcccaggt gatgttgctg cggtgcagga aaccttcgat accttcgacg
     4981 ttaataaaga cgccggaatt cgtgtgcttc gtgacaatac ctttaataat gtgaccgatc
     5041 ttgatcttgt cgccgatgtc cttccagacg gtcgcgttct gttcgtcctg gaacaccttg
     5101 cgtgaaaccg tgcccgtctt gcggcggtcg ttcaggtcga gaattttaac gaggtaggtc
     5161 tggccaacaa tgttgatctt cttggcgccg cgctcgcgtg ggcccagcag gccaacctga
     5221 gtatgaggca ggaacataaa tacgccttcg acgtttacca tgtagccttc gcgcatcgag
     5281 cgcttcacca gtgcgttgac cggcacctgc ttttcaaatg cttcttttac tatttcccag
     5341 ccgcggcgga gttcgagttc gcgcttcgac agctgcacca ggccggtttc ctggtcgtgc
     5401 gagcggacca gcgcttctac tgtctcgccg acagcgggtt caacgtcaaa ttcgcggatg
     5461 ccgatgcggc cttctgactt gccgcccatg tcgatgatgg caaattccgg agtgataccg
     5521 acgatcttgc cactgacgat cgcgccagac cggatatctg cgacagccgt gtctgtcttg
     5581 aacacgtctg ccatcgagac gttactgtct tgaaaaagaa ccttgcgcgt tgccatggga
     5641 acccctaaag tgtggcgacc ggtcgagccg ttacagccgc gagttgcgga tatacgccag
     5701 cattaaatct gccacttgct cctgagtgtg agacgaggtg tcaatgaaaa tggcctcggc
     5761 agccggtctc aggccgccaa actcgcgact ggcatcgtcc ttgtcgcgcg cctcgatctg
     5821 gtgctgtacc tcatccagtg aaattgtagg atttttttcg cggaattctg ccaggcggcg
     5881 ctcagcgcgg atgcggctat cagccgtcag gaagaactta aagggagcct gcgtaaagac
     5941 ctcagtcccc atgtctcgcc cgtctgcaac gagcgaatag cgctgcgcag cggcgacaag
     6001 tacctcgcgc acgcgctcac gcagataacg tgcatcggcg accggcttga tacgctgcgc
     6061 cacctcgacc gaccgcagct cctcccccac ctcaacgccg tcgaccaaga cgtgctgcct
     6121 gccgtcggta ccaaagcgga tttcaagcgg cagggcggcc aggtacgcac ttagcgcttt
     6181 atcctctttg acggcgttcg cccattctgt gctcaacccc agccgctggt actcacggta
     6241 accggcccag gtaaaggcac ggtacagcgc cccggaatcc acctggttgt agcccaggct
     6301 tttggccacg gtgtacgcca cggtcgattt acccgagcct gccgggccat cgactgtgat
     6361 cacctgtggc tggacatgcg aagccgcagc aggcggcgag aactcgagcg ttgcctgcag
     6421 cttctcataa aaggaaggaa aacttgtcgc tacccattca cgcccctgaa tttgtagcgc
     6481 aggcgccacc tcggtttcag ctgcgtgcgg gttacggata tgccgtgaaa tcaggtcggc
     6541 gatttcaaag gacatcacga tgcggtggtc catatcgggt tcaatagtac ccgtcagcgg
     6601 acggcctggg tcgccgtcga gcgagagccc atcttcaaat tcttcgacat gcacatgcga
     6661 ttgcgcgaga ttcctgacca tgatcgagat gcggtccgac tctttgaccc gcaactcttt
     6721 cgcattgcga tacgagaact ttcccgcggc gaacgtgccc gcaatcgtca gcacgggcaa
     6781 ttcatcgatc agcgccggta tactctctgg tggtatggcg acgccgtgca ggcgtgcatg
     6841 cttcaccagc acgtcaccgc ccttctcgcc gcactcgtcg cgttgcggca ggacctcgat
     6901 cgctgcaccc atgtcgcgca gcgcctgcag gtagcggtcg cggtaaggat tcagcagtac
     6961 atggcgtatg agcacttcgc tgcctggcaa aaggctcgca cccacagcaa aaaaagcggc
     7021 cgacgaaaca tcgccccaga tacggtattc gcgcggctgg atagtcacac ccgcatcgag
     7081 cgtgagccgg aagccttcgc cgcttctttc tttttttacc accgccccgg cgtggcgcag
     7141 catgttctcg gtgtggtcac gcgacggtat tggttcatct aatacgagcg aggtctgcga
     7201 ggcgattgct gccagcatca cggcggattt cacctgcgcg gaacccagcg tctccgtgaa
     7261 ctcgattggc ggcagcctgg taccggtgac acgcaccggc aggcagtctt tttccccaag
     7321 ccattcgaat tttgccccga ccttttcgag cggcccggta acccgacgca tcggacgttt
     7381 acgcaggctc gcatcaccgt cgataaccgc ggtgactccc tcgaggccag cgataagccc
     7441 catgatgagc cgtgaccccg tgcccgaatt gccgcagtcg atgaccccgg cgggagattt
     7501 caggcctttg agcccggggg actcgacgcg gaatgagcgg ttgccgaggt cggtcacctt
     7561 cgcgcccagc gcctgcatcg cgtgcagggt gttcatggtg tccccggcat cgagaaagtt
     7621 gtgaatggtc gaggcaccct ctgcgagcgc ggcaaagatc gccgcccggt gtgaaagcga
     7681 cttatcaccg ggaaaagtca gggttccgcg catcggcatg ccagctgaaa caagcggcag
     7741 agagtcggcg taaaggattt caggccgggc gcagccgctg cagggtcacc cggcaggctg
     7801 atggcctgct aaatagttta caccctgtct ttgaagcgca ttttagccgg cgcttccgcg
     7861 ttgagaggcg ctcagcacat ggttgaagca gaactcaaag tactcgcagg ccgttcgaat
     7921 cttgagctcg ctcaggaaat cggcgacgaa ctcggcatca aggtcggggg cgttgaatgc
     7981 aaacgcttca gcgacggcga aatttctgtt aagatatcag aaaatgtccg cggcagtgat
     8041 gtttttgtca tccagtctac cagcaacccg gcgaacgaca acctcatgga gctcatgctc
     8101 attgtcgatg cgctccgtcg cgcctctgcg cggcgcatta ccgcagttat tccttattat
     8161 ggctacgcgc gccaggaccg caaggtcgag ccccgcgttc ccatttccgc aaaggtcgta
     8221 gcaaccatgc tcgaggcaat cggcgtcaag cgcgtgcttt cgatggacct gcacgcgaac
     8281 cagatccagg gctttttcga gatcccggtc gatcacctgt tcggcgcacc ggtgctggtg
     8341 aagtatttta aagagctgaa cctcagtgac ctggtcattg tttcgcccga ttccggcggc
     8401 gcggagcgcg cacggttctt cggtaaacag ctgggcgcca acatggccat catagacaaa
     8461 cgccgcgagc gcgccaatga ggccgaagtg atgcacatcg tcggcaacgt cgaaggcaag
     8521 aactgcctgc tgatcgacga catgatagac accgcgggca cgatctgcaa ggccgccatc
     8581 gcgctgagag aaaagggtgc gctcaagatt atcgcggcgg caacgcacgg tgtactctcc
     8641 ggaaacgcga tacagaacct catcagctca ccgatcgatg aggttgtagt cactaatact
     8701 ctgcaaattt ctgacgaaaa aagattcccg cgtatgcgcg tgctgtcagt ggcttcactg
     8761 tttgccacgg caatcaagcg tatccacgaa gaacaatccg tcagctcatt gtttctataa
     8821 accacaggag aagaaaatgg aaaaggcaac actcaaggct cagaaacggg acggcaaagt
     8881 cggcggcaaa aacctgctac gcgccggaat tatcccggct gtgatctaca accacggcaa
     8941 gagcgaaagc atccaggtgc tcgaaaaaga tacacgcaag ctgtttgcgc acggtatttc
     9001 agaatcgcgc ctgattgacc ttgatatcga cggcaaaatt gagcaggtat tcgtaaaaga
     9061 ttaccagtca cacccggtaa acgagatgct ccagcacctc gacttctacc gtgtttcgca
     9121 ggacgaaaaa ctccgtacgc gtatcgctgt gcacgttgaa ggcaaagccg aaggcgttaa
     9181 gaatggcggt gttctcgaag tattcctcac cgaggtacct tacgaaacat acccgcgctt
     9241 cctgacggac ttcctcacca tcgacatttc gcacctgaat attggtgatt ctatccacgt
     9301 cagcgacgtc aagctgccgc aggatagccg tatcctgatg gatgctcaaa cagtaatctg
     9361 ccacgtcacg catgctgcga aagaagaagc ggctgctccg gctgctggtg ctgcacctgc
     9421 tgcagcggct gcacctgcag cggcaaaggc agagccggca aagaaataac cggcttctgc
     9481 ttcccgacgc agactgaatg gcgctatcga atcttaaact ggttatcggc ctcggcaacc
     9541 ctggggatcg ttacatgaat aaccgcaaga atatcggctt caaggtagtc gatgttcttg
     9601 cgaataacac cggcatccat attaagacaa agaagaagaa gtcgctgctt ggccagggcg
     9661 atttcgaagg ccatgacatc gtactgctga aaccgcagac tttcatggac ctttcgggtg
     9721 aagcggttct ctacatcgct tcattcctgc gcgtccaggt gcagaatatc attgtgatcc
     9781 atgacgatat caccctaaac tatggcgatg tcgtggtagc ggttggtcca cctgagatcc
     9841 cgaatatcgg cgttgagtcg attgcgcgcg gcctgaaatc agacaagttt gtgcgcatcc
     9901 gtatgggtgt cgggcctgtg aaaatccggg gtaaagaatt ccccaaccca aaagagaatg
     9961 agattaaagc attcatgctt tctgacttta cactcgaaga gaacatgaag atcatcgaag
    10021 tgattaacga cgccgaagct gccctgcgct cgattctgac gcaggacatc aaggacgtcc
    10081 tcgcccgctt caaactcggg gcgaaatttt ccaagaatac cgcctgagcc gcggcatgtc
    10141 actccgccag gccctggcca ccttcgcatc cgttgcacat aagccgcact ggttgattgc
    10201 cttttcagcg ggagccgaca gtgtattgct tctcgacctc gctgtcgagg ccgctctgcg
    10261 ctctgccgat atacgggtca ctgcatttta cctgcatcac tactctactc ccgtcgagag
    10321 cgaacgcagg cgggcgataa gtttcatggc aagcagggca aggcaactcc tgggagatcg
    10381 attcgcgttc tgtgaattgt ccagtgatat taccatgcgc gcacacaggc tgcgccgcag
    10441 ctgggagcac accgcctcgc tcgtgcggcg gcggcatctc gagcgcctgg cgcgccggct
    10501 gggcggagca caggttttta cgggccacca gctcagcgac tacagggaaa ccctgcaact
    10561 gcgtgccgaa cggtgcgtac cccggtcagc atggcccgcc ctgtcagtac acgacagcaa
    10621 cacggacttc ctgcgcccgc tggccttctt cacgcgtgaa caggtccgcg cgtttgcgcg
    10681 cgaccggggg ctcagctggt ttgaagatcc ttcgaacagc gatacgtcta ttgcccgcaa
    10741 ccggctgcgg gcaacggaac aacctggcga aaatatactc gtgccgccgg agcattgtac
    10801 ggacaccagt cagcccaagc cacgacgcgt gcacccgcgt gaaatgcgca tgcctgagac
    10861 ggagtggcgc aaccttgcac cggtcatgcg cgcccgcgct gtgtaccagg catggcagca
    10921 gctgttcatc gtgaagcggt ttacacgtaa cgattttgcg cgtgcacagc gactaccctt
    10981 cagtctgcgg ccattttttg cgcatgcaga aaattttcca gaaggtgaat ttatagtttt
    11041 tcgccgtggt ctcggttcaa tgtcccttaa cagccgcgcg acacagaaca gtatgcgggg
    11101 cgatgccatc acgcgcgcag taaaactgcg ccagccatac gggcataagg cagtcaccaa
    11161 gatcttcagc gagcgacgcc tttcgccacg ccagcgacga ttgacatggc tcgaaatggc
    11221 agacagtccc aacgaggcat tgcgtatttt tttccccgat ggagcagtac tgtgaagttt
    11281 cgtcgcgtgg tagctcccgg caagacgctg gtcgttgcgg gcattgatcg caggtttact
    11341 gttttcacag cctggttcct gcatctctgg caacagcgct tttccacacg accgtttctt
    11401 gccgctgcca cacgacctgg tgacctcggg gctccgttac tgaattccct ggcagagcca
    11461 gtggaattgc cagggagctt ttttcccctg ctctggtttc ttatcagagg gcgcttcagc
    11521 acattgatca tcctggatcc cgatttgcgg gcaacgctaa agatccgcat cgccgcacgc
    11581 cttgccggca tcaggcaccg cgcgggcttt gccccgctgc gacgtatcac tggtctgaac
    11641 ctttcgctgc cgtttaataa tgagaaccac cactacgtcc accagttgcg aacttttttt
    11701 gaatacatta ccggggaaaa aaccagcggc tggagcacac cggatttccc cctggccgca
    11761 agcagtggtc tggccgacgt gccggcagag ccgttcggca tcattgccta caatcccgat
    11821 gaaacggaat cggagcacct cgaatcgcag ctaaaaaaac tcactaacct gatggcacga
    11881 gcatcgcact gcgtgctgat tctgcgcagc cggcacctcg gcaggcaggc gctcacccgt
    11941 tttgcccggt cattcagcga gataatgacc gagcaggcgc tcgcaaacac gacactgctg
    12001 gtgctgccca ctgaagagac gctggttggc ctgctgcgcg aggccgcctg gattgccgcg
    12061 gccgatacgg aattgctgaa cctggcggca ctcttcgccg tgccctcagt agctgttttc
    12121 gggccgctca atgaacgggt ctggcaaccc ttctcggtgc gcacgcgtgc gctgacaggc
    12181 gaatttgact gccgcccctg caccgcttat cccggcagag tcgaatgcag caacagcgtg
    12241 ccctgggcat gcctcgccgg ggccagcgcc gaacttatgg ctgccacgct tactgctgta
    12301 ctacggcgta aacgtcatgt gacataattt gcttgccggg cgggcgcatt ttggccgcaa
    12361 ggaacctgcc acggtatttt taaccggtag tgcgccgcat gcgtctaata cagcagatgg
    12421 attttcgttt ctttccggcg ttgctgctct tggtggtggc gttgcctctt tctgcggctc
    12481 cgggcaagcc gggagcccgc gccgcaggtc gggctgacaa gaagcctgct gtcgatttgt
    12541 ttgccgaggc aaaagacaag gtactccgct ggcaattcgc cgaaggcgac attctcgaac
    12601 tcaagaaatt ttctgaccag gtcgtccgaa ccggggctag ctcggtgaag cgttcagtct
    12661 tccaccgcgt attgcttgag gcgcgcacac cagacccggc aaaaggtttt ccgctcgaag
    12721 ggacttttac cacactcatc aagagcggcg agacgaaaaa tgtctatgcc gaagccgaaa
    12781 aatacacggc aagcttcgtg ctgcaaccag ccggacagtt tgtggccgat gctgaccagt
    12841 acatgccgaa catccgctcg gtaccgagct tttctgaaag ccgcgacccg gcgctaaaag
    12901 aagaagccgg catggagcct ggaaccacat gggagaagcc gggcgcagaa gtcatgcgct
    12961 ttgacaagct cgtcaacgta cccttcaatg tacgttatga ataccggggc ctggaaaacg
    13021 tcaaatcgga agaaggggaa aaatcctgcc acaagttcat ttccaattat gaactcaatt
    13081 acggtgacaa taacggcacc ggcccgcgcg ttttcggcta cgtgaccgcg atctggttct
    13141 gggacgcggc acagggcata ccctactacg cgcaggaaga ctacaacgtg atcatcgtca
    13201 atgaacaggg ccttgccaat gagttcaaga tcaagagccg cagttattac cggaagttcc
    13261 gggcgcgtgg cgacgaggcg aaggtcgcac tggcccaaaa aattaaagaa gacctggtca
    13321 aagacaaccc acagctcgat gtacgcgtaa cagaccatgg agtggcgatt gctctccccg
    13381 atgttttttt cgcgaccgat agcgcaaaac tcagcagcga tgcaaagaaa gtaattgaac
    13441 gtgtgggtaa gaccctgcga agtctgcaga accggcatat ccgcgtgcgc gggcacaccg
    13501 acagcacggg tgatgaaaaa tacaaccagc aactgtcaga aaaacgcgcc gagtcggtgg
    13561 ctgactacct gatcgatgag gcggagctga atcctgattc agtcagttac gacggcaggg
    13621 gtgcacgcga tcctgtagcc gacaactctc tcgaagaagg ccgggcccgt aaccgccggg
    13681 tggaaattat cctgctcgat aggtgactca gcgaaacacg ttgccgttgc ggtcgatgcg
    13741 ggcaaagatg atatcaggat tcttgcgatc tccaccggaa aaggcgagca caagatcacc
    13801 ctggccattc aggcgcatcg acgcgagttt cagtccatca gccggcagga cacgcgtgta
    13861 cagccttacc cctgaaggcg atgtgcaaag tacgtaatag tcagtcattt tccccggatt
    13921 ggaatgcgcc tggccatgca gcgctccatc ggtcttaccc gcaacgcagg cacgcgccga
    13981 atttgccgtc atctgctcaa ttgtcatcgc cgcttcaccc gcaaaattct ttgtccaggt
    14041 agttttgcct tctgcatctt cgccccaaag cgcaaccccc ttcctgtacg agagtttatt
    14101 gaaatcgcct gcaaggtacc gcgtaccggc aggcaccact gtgaagctgc gccagccgcg
    14161 tgacttcgca atgcgcccga tgttcttgcc gtctgcgccg ctgtaggcaa ggttctgcgc
    14221 taccagcgca actgaaccgt cgtcgagcag caaaagttca cccgtgcggt cgctgcgtgc
    14281 aatttcagtg ccgtcagaaa ctcccgaaga gttgatacgc cgcaggctgc gcttgccggc
    14341 aaattcggca tagacgagcg tatt
//
LOCUS       DAOQJF010000036        14223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2063_length_14223_cov_4.760375, whole genome shotgun sequence.
ACCESSION   DAOQJF010000036 DAOQJF010000000
VERSION     DAOQJF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2063_length_14223_cov_4.760375"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLF85_03375"
     CDS             complement(<1..261)
                     /locus_tag="PLF85_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE18729.1"
                     /translation="MKKQPDTVAVVSSYVGQGDSFHGDFQLTGALRVDGTVSGTVRTT
                     SQVIIGPTGHVKTNVEADSVIVSGRVDGNIYALDFVHLMKNAR"
     gene            complement(271..1395)
                     /locus_tag="PLF85_03380"
     CDS             complement(271..1395)
                     /locus_tag="PLF85_03380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE18730.1"
                     /translation="MKQSSSKRRAKKGQSAGAPAEIGVKIDPGSAPHQVKGLGAWAMR
                     GKATVAMAAPAIGRFARSQQKILTWLKKKRNERLTLMFIPHNELKIRNYHISNLTLII
                     ITSFLGLVLLVSAFLVIRHNSTIQEVDKLRVSHKDAEVQFAKVRQEIIELSKSYEDIR
                     QNLNSLYSLSKSGEKSAAPTQGSDSFAAQLAELQQKTSQSKDPEKIPLEIFLMNRMLH
                     DMEISRGSLGQVQDYLKKRERVLKNTPTIWPVQGYIINPFGFVRMAHRLNVGYNPGVD
                     IAVPHGAVVQATAPGIVVSINKEANLTYTVRIRHNYGYETVYKGIEMVTVSQEEKVNK
                     GEHIGHVGHADDNFESILHYEVHIGVEAVNPMPYLPLAGA"
     gene            complement(1496..2734)
                     /locus_tag="PLF85_03385"
     CDS             complement(1496..2734)
                     /locus_tag="PLF85_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE18731.1"
                     /translation="MSGNGRISVLIHGAAAHISWCTTGLTEKSITDRHFVTDSERLAE
                     VLRNHLPEIALVTCVDPVACGDVVMLCRRANPGILVIVVKAGENPVSPLDATSRSVFA
                     VVSQDNDGVEFAAAMKKAVAFVLERRKLQEKSERRRLRREHPASWLMEREARERDNKL
                     EFAASLIRNILHSASQGLGIGSVLTYIDLLQMMMPQLQKENEAFASLLHNARASRAWL
                     ASFENILAGLHKQYPREVVHDSDIEAVIETALAVTEKFRAVKGQVIDRAHFQALGSLH
                     GNREALQEILSELLLNAYKYSPADSAVSIMSYSTNEISAITVVNETEQKIGGQVGIPA
                     ELEEKIFEPFFRVNNTMDDRFAEQKYGLGIGLTLADHAALQCGGRLYVYELEAGESFA
                     SGVAGTGRRVVAELVLQKVQ"
     gene            complement(2731..3087)
                     /locus_tag="PLF85_03390"
     CDS             complement(2731..3087)
                     /locus_tag="PLF85_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE18732.1"
                     /translation="MSRILAVDDSEPMRQMVEQTLRSGGHETVLAVDGKDALEKFRAG
                     EFDLIITDINMPVMDGYEFTRVVREFDDQTPILALTTEGEQAKRTSGQEAGVDGWIVK
                     PFKPAQFLAIVKQILE"
     gene            complement(3180..3836)
                     /locus_tag="PLF85_03395"
     CDS             complement(3180..3836)
                     /locus_tag="PLF85_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE18733.1"
                     /translation="MLTGSQGNDYRIVLADDHSILRAGVKVLIESKPGFKVVGEVSNG
                     QELLDLLPQTPCDMVILDLNMPVMNGIETLEYLQMHFPKVAALVLTTHKEKPFLKRSL
                     AKGAKGYLLKEETHDKLLSAISDIRQGKRVISAEMTNLIVEDYSTDLSSPLSADLLTQ
                     REKEILSYTANGLTSKEIAERLDISARTVEAHRANIREKLGIETMSDLVKYAMDHNLM
                     "
     gene            complement(3939..4295)
                     /locus_tag="PLF85_03400"
     CDS             complement(3939..4295)
                     /locus_tag="PLF85_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE18734.1"
                     /translation="MGNILAVDDSEPMRKMVDLVLKSGGHQVTLANDGVEGLQKFKAG
                     KFDLIVTDINMPNMHGIELIKAIRALNPDIPIVALTTESEDEMRRKGAEAGADGWVVK
                     PFKPAQFLDIIRQILS"
     gene            complement(4375..7788)
                     /locus_tag="PLF85_03405"
     CDS             complement(4375..7788)
                     /locus_tag="PLF85_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE18735.1"
                     /translation="MTSSQAILHELSVLIVEDSESDAALVEYELKSRGYELRTLRVDT
                     SRALKAALQNSWDVIISDFRMPQFNAITALEILRESGKDIPFILVSQAIGEGVAVELM
                     RAGAADYIMKSSLKRLGPAVEREVREARVRQQARQAAQALEESERRLRAAQQVARLGN
                     WETDLETLVVNWSDETYRIFDLDADSFAPTHHSFFDYVHPDDKQLVDDSFKSSFTSEG
                     EHAITHRIITAAGRLKHVQERWRVERNAAGKPVRAFGTCQDITERFELEGQLQEREAY
                     LDSIIRSQINGIIVVDTKGEITVANESAARILRLKHDEVTGRYFQEADYWKQIDHNFE
                     PLPPETLPLSLALTAGQSVSGFEHGIVFPDGSTKWLSVSCSPIWAASGRLIGAVASFL
                     EITEAYLQRERNLQQLQRLRLQDEAINSSLSGVIISDARMPDMPVTYINRAFTTITGY
                     GAEDIIGRNCRFLHGDDHDQPDLIRLRAALKRREGGEYILRNYRKDGRQFISQLRIAP
                     VRNDANEVTHFVAIQTDITEKFIAERELEETRDRMEMALEGAQLGLLDYRITERRLYV
                     NERFAEMLGYTVEEFYNVFADPWQMIVPEDREEAVRISNEHFSGKNPRIDTQHRLRHR
                     SGRVVWVLSRGKVVERDAQGTPLRYTGTILDITVTKEAEAELNRLSRLLTEAQRIARM
                     GAWELDVETGQTTWTDEVYRIHEVQPGTSYAREAAIEFYHPEDRPLLIAALQRTIETG
                     EPYDLDLRFITARGNRLWVHTSGYPVFEGNRVTRLTGMFQDITEAKNAAIQLKQSEQR
                     LALALIGGDLGLWDWDIVSGEAFFDERWCGMLGYAVTEVRPSYEGFSGLLHAEDIPGV
                     QAKLSGCFGDPTQGYEAEFRMRHRDGRWIWILARGKIVEWGSGSKPLRMVGTHADITL
                     RKQQEQRILELSQRLIDISEMERAEISSELHDVLGQSLVLTKLNLQKFLGDQGMRTPE
                     NERLLLEPISDTLKKAREISRRLTPSHMKKVGLVLALEDMLQSAASLSKVKIETDLEA
                     LEGFFPENWSIACYRVVQESVTNSLKHSGATRITVRARLVDKNLELTIADNGNGFANT
                     ADSSGIGLSLMRERVRGLRGHLFIESAPTGLTITALLPQQVSAPDAVSP"
     gene            complement(7785..11270)
                     /locus_tag="PLF85_03410"
     CDS             complement(7785..11270)
                     /locus_tag="PLF85_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE18736.1"
                     /translation="MAEISSSQAMIFCAVISLLLSLWFLFLAVSRVCPHRPMIAWAWY
                     LLLQAAGFFSIALKNSMPELYAAMPGSILLVASSVFLVRAYNGVLSFHQQTRWIFVLI
                     GLATAGLGYFSWLLPHAPARVALSGIILGLVSVHVARVLVLAALAQHWRIAGAVATGF
                     IILGVAHFTRFAAAASAWPLAEPSKLPPAPTPLLVYGPLVLWSIVPSLGFLAMQLDRL
                     RREQQADQLRTTGIVDSVDGIVWEADARTVNFTFVSGKAIRLLGYSVEEWLTPGFWIR
                     TMHPDDVTWVPEYCQAKSRELAPHDFEYRLIAKDGHIVWLRDIVSVAAEDGNARWLRG
                     VMIDITESKKAAEKLQRLTFMYELLSNCRRIISQTSTESDLFSAIVRLCVTNNYIKLA
                     WIGLPEDDSMLVRIAAAAGSVTAYLDDLRVSMSPRLAEGRGPTGTAMRESHDVVVNYP
                     DRLPADQWSERRQKHGLHSYAAITFRLSGVRMGSLNLYAAEEGFFTADIIGLFDQLGT
                     DLSAALDRIEAERWRSFAEQSLRENQMLLDFALEATVDAVWDYHVPSGTVRFFRHWEA
                     MLGYEQGELQNDPLAWEAHVHPDDLRASDAMLESYRRGDIPAFDMRIRLRNKFGNYLW
                     VKDRGIVVERDESGEATRIIGTLKNIDAEVKKAEESLQNAALLDAIFDASPVAIAVLN
                     HRRELIKVNPAVERIARVANDADLKKAIAERMFFYPDGQPYPRGDVPAPLALPRFDQD
                     YSAVFGFRDREKEDLRWVSMTTRYLADVGLVVCISTDITSVIESETALRESEQKFRNI
                     FESAPIGITLFNSAGAPISLNPEVKRQSEIESTVSMDDVADWHFIDESGNRISLEDMP
                     LSVSVREKRPVRDFIFGRQFSEKVRWFTSTVVPMTEIGMYAGYLVELTDLVNAKREIS
                     GLAATLEETVAQRTRELQEINAELEAFSYSLSHDLKAPLTRAESWLNILDHEFRSALG
                     EKGKGMLVYVRNEIGAMKRMNEAMLALSRVARLNIQPVEASLTQKAQEIVQQLREENS
                     DKVIELALPENSIVYADPELLRILIRNLLENAVKFSQGKDPIRIEISCAAERQHFLCA
                     VRDNGVGFDMQYADKLFAPFQRLHAEGEFPGTGIGLATAQRIVHRHGGKIWAQSQPGV
                     GTTIYFTLPRGGETP"
     gene            complement(11288..12151)
                     /locus_tag="PLF85_03415"
     CDS             complement(11288..12151)
                     /locus_tag="PLF85_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNE18737.1"
                     /translation="MSDKAFPGIAAAIKRLTGLHIEPEKYYLFEHRFPEVMKEHGISD
                     FDQLLSAIEKGTDNKLLGRVIEKITTHETRFFRDESIFDALAEQILPEWKDRNALPGN
                     ELQYPPLKLWSAACSTGQESYSIAIILAEFHPQLVKKAQIIATDISQESVERAAAGRY
                     TNFELSRGLPEKFKRKYFSDVEGGAMINRTLLPSIDFRTHNLITQPAPDKFDIIFCRN
                     VAYYFAQDTRKQLFEKMQASLKSDGVLILGSAESLSGYLTDYVLREFGLARYYELNAA
                     NVTMFARKKKS"
     gene            complement(12151..13197)
                     /gene="cheB"
                     /locus_tag="PLF85_03420"
     CDS             complement(12151..13197)
                     /gene="cheB"
                     /locus_tag="PLF85_03420"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNE18738.1"
                     /translation="MKTQVLIVDDSEVYRLFLRQTLATEPDLEVVSVASNGRLALPRV
                     NHYKPEIVLLDYEMPELDGLATIPEIKKLSPQSRIIMFSSHTTKGARLTLQALELGAD
                     DFITKPAGALGGNVAAELRNRIVERIRAFTQRHEHSQAPTPVVSPPPSPRPTVKGRKS
                     FRYCVIGISTGGPPALRRLFRLIPPDIRGSILVVQHMPPLFTAQLAESLNEESRLKIV
                     EAANGVPAEPATVYIAPGGKQMKLEPRGTGAVIRIEESNPGELCKPSANILFNSAAEF
                     FASDTLAVIMTGMGEDGYLGMKNLAARGAYLMAQEAADCVIFGMPAKPVRDGIVSESH
                     NVEGLAAKITEYLR"
     gene            complement(13219..>14223)
                     /locus_tag="PLF85_03425"
     CDS             complement(13219..>14223)
                     /locus_tag="PLF85_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE18739.1"
                     /translation="GVTIGALKLPVLLWTRYVVLKQVGEANQAMKEMGEGDFTKPIDV
                     RDESETGRLLESIQIMNTNVAGLIFQMTETGKDLNSGAKTLADSSQNLSTGIEEISQQ
                     TSSISSAANQMNQNLQVVSSAVEEMSISIGEVATKASEGARVSKEAASIAQDAQGLVR
                     ELGTGADSIGKVIEIISKIAEQTNMLALNASIEAAGAGEAGKGFAVVASEVKELARQT
                     AASSEDIKKQIQAIQKSTDQTIQAIGNIAGIINRVNEGNASIASAVEEQSMTTKEIAS
                     NVNQISEASAEINRNISGISQAVTDSAKDAHKISELSSGLKRLSDFLGSVVMEFKVIK
                     "
BASE COUNT         3192 a         3995 c         3572 g         3464 t
ORIGIN      
        1 acgggcattt ttcatgagat gaacgaaatc gagcgcataa atgttgccat caacacgacc
       61 cgacacgatg acgctgtcag cttcaacgtt ggttttgacg tggcctgttg gcccgataat
      121 gacctggctg gtggtacgca cagtaccgct gaccgtccca tcaacgcgca gcgcaccggt
      181 cagctggaag tcaccgtgaa agctgtcacc ctgccctacg taactcgata ctaccgcgac
      241 cgtgtctggc tgttttttca tgattgttcc tcaggctccc gccagcggca ggtacggcat
      301 cgggttcaca gcttcgacgc caatatgcac ttcgtagtgg agaatgcttt caaaattatc
      361 gtcggcatga ccgacgtggc cgatgtgttc gcccttgttg accttttctt cctgcgagac
      421 agtaaccatc tcgatgcctt tatacactgt ttcgtaacca tagttatgcc gaatgcgcac
      481 ggtatacgtc aggttcgctt ctttgtttat actgaccacg atacctggcg cagtcgcctg
      541 cacaaccgcc ccgtgtggta cggcaatatc gacaccggga ttatagccaa cgttgagccg
      601 gtgcgccatg cggacaaaac caaacggatt aatgatataa ccctgcaccg gccagattgt
      661 cggcgtattc ttcaaaaccc gttcgcgctt cttcaggtaa tcctgcacct ggcccagcga
      721 accgcgcgaa atttccatat catgcagcat gcgattcatg agaaagattt ccaatggaat
      781 cttctcaggg tctttgcttt ggctggtctt ttgctgcagc tctgcaagct gggctgcaaa
      841 actgtccgag ccctgggtgg gtgccgcact tttctcaccg ctctttgaaa gcgaatagag
      901 ggagttcagg ttctgccgga tatcttcgta ggatttgctg agttcaatga tttcctggcg
      961 cactttcgca aactgcacct cggcatcttt atgcgaaacg cgcagcttat cgacttcctg
     1021 tatagtactg ttatggcgta ttacgaggaa agcgctcacg agcagcacca ggcccaggaa
     1081 cgaggtaata ataatcagcg tcaggttgct gatatggtaa ttgcgtattt taagttcatt
     1141 gtgcggaata aacatgagcg tgagtcgctc gttccgcttc tttttgagcc atgtgagtat
     1201 cttctgctgg ctgcgcgcga accggccgat tgccggcgca gccatggcaa ccgtcgcctt
     1261 tccgcgcatc gcccaggcgc cgagcccctt cacctggtgc ggcgcagaac ccgggtcaat
     1321 tttcacgcca atttctgccg gggcgccggc actttgccct ttttttgccc ggcgcttgct
     1381 cgacgactgc ttcatggcct gccgtcagct ttgcagcact tttcccggcg tcaattaata
     1441 ggagtgtctc cgaagctccc ccgaaagcgc cgacaagtcc cgataggcgc cctggctact
     1501 gtactttctg caggacaagc tctgcaacga cgcgtcggcc agtacccgcc acaccagagg
     1561 caaaactttc gccggcttcg agttcataga cgtacagacg cccgccacac tgcaatgccg
     1621 catggtctgc cagcgtgagc ccaataccga gtccgtattt ctgttcggca aaacggtcat
     1681 ccattgtatt gtttacccga aagaatggtt caaatatctt ttcctcgagt tccgcaggta
     1741 taccgacctg tcctccgatt ttctgctcag tctcattgac caccgtaatc gcgctgattt
     1801 cgtttgtact gtaagacatg atactcactg ctgagtccgc cggtgagtac ttgtaggcat
     1861 taagtaacag ctctgaaaga atttcctgga gtgcttcacg gttaccatgc aatgacccga
     1921 gagcctgaaa atgcgcgcgg tcgataacct gccctttgac cgcgcggaac ttttctgtta
     1981 cggcaagcgc tgtctctatc actgcttcta tgtcgctgtc atgcacaact tcccgcgggt
     2041 attgcttatg cagtcccgcc agtatgttct cgaaagatgc aagccaggcg cgcgaagccc
     2101 gggcattgtg cagcagtgag gcaaaggctt cattctcttt ctgcagctgt ggcatcatca
     2161 tctgcagcag gtcaatatag gtaagcacag aacctatgcc gagcccctgc gatgcggagt
     2221 gtaatatatt tctgatcagt gaggcggcaa attcaagttt gttatcgcgc tcgcgggctt
     2281 cacgctccat caaccagctg gcggggtgtt cgcgacgcag cctgcgacgt tccgattttt
     2341 cctggagttt gcgacgctca agaacaaatg ccacagcctt tttcatcgca gcggcgaact
     2401 cgacgccatc gttatcctgc gaaaccaccg caaaaacgct cctcgacgtg gcatctaatg
     2461 gtgaaaccgg gttttcaccc gccttaacga caatgaccag tatacccggg ttagcgcgac
     2521 ggcaaagcat caccacgtcg ccgcaggcaa caggatcaac gcatgtcaca agcgcaatct
     2581 caggcagatg atttctcagg acttctgcca gccgctcgga atcggtcaca aagtgcctgt
     2641 cggtaattga tttttcggtt aaaccggttg tgcaccacga gatatgtgca gccgcaccat
     2701 gaattaatac gctgatgcgg ccgttaccgc tcattccagt atctgcttaa caatggcgag
     2761 aaactgtgca ggcttaaaag gcttcacaat ccacccatca acaccagctt cctggccaga
     2821 agttcttttt gcctgctcac cttctgtggt aagcgccaaa atcggggtct ggtcgtcgaa
     2881 ttcccgcacc acgcgcgtga attcatatcc atccattacc ggcatattga tgtctgtgat
     2941 aataaggtcg aattcgccag cacgaaattt ttccaaagcg tctttgccgt cgactgcaag
     3001 cacggtttcg tgcccgccac tgcgcagtgt ctgttcgacc atctgtcgca taggctctga
     3061 atcatctacg gcaagtatcc tggacattgt taatctcccc gcgatctgga atctatcaca
     3121 aaccgacgct acggtcaata agtcgttcag cggacatctg tcagtagaaa tacttaacgt
     3181 tacatcaggt tgtggtccat cgcatatttc acaaggtcgc tcatggtttc aataccgagc
     3241 ttctcccgga tgttcgcgcg gtgcgcctct acggtacgcg cactgatgtc cagccgttcg
     3301 gcaatttctt tgctcgtaag cccgttggct gtataactaa ggatttcttt ctctcgttgt
     3361 gtcagcaagt cagctgatag cgggctggaa agatctgtag aatagtcttc gacaatgagg
     3421 tttgtcattt cggcggagat tacccgctta ccctggcgaa tatcgctgat cgcagatagc
     3481 aacttgtcgt gcgtctcttc tttcagcagg taaccttttg ctccctttgc gagcgagcgc
     3541 ttgagaaagg gtttctcttt atgcgtggta agcaccagcg cagcaacctt gggaaaatgc
     3601 atctgcaggt actcgagcgt ctcgatgcca ttcatgaccg gcatgttcag atctagaata
     3661 accatatcgc agggggtctg cggcaacagg tcgagtaatt cctgcccgtt ggaaacctca
     3721 ccgacaactt taaagccggg tttgctctcg atgagaactt ttacgcctgc gcgcaggatc
     3781 gaatgatcat cggcaagcac gatgcggtag tcgtttccct gtgatccggt cagcatgttg
     3841 ttggccttcc tccgcttttg ccacagtcgt caagtctaaa gaaatacccg tcacaaggcg
     3901 gctgcagcca aaaattgcgc aggaccattg tccgcgcgct aagaaaggat ctgccggatg
     3961 atgtcgagaa actgtgccgg cttgaaaggc ttcacaaccc agccgtcagc gcctgcttct
     4021 gcaccttttc ggcgcatctc gtcttcggat tctgtggtaa gagcgacaat aggtatgtcg
     4081 ggattgagcg cccggatggc cttgatgagc tcaataccat gcatattcgg catgttaatg
     4141 tcggtcacaa tgaggtcaaa tttgccggcc ttaaatttct gcagcccttc tacgccgtca
     4201 tttgccagcg tcacctggtg cccgccgctt ttgagcacga gatccaccat tttgcgcatc
     4261 ggctcagagt catcaaccgc aagaatatta cccatactta tctatcttcc taggacgttg
     4321 catttctggt atttaacgac actctgtaaa gagcgtgagc gtctttgcgt tccttcaagg
     4381 gctgaccgca tccggtgcgc tgacctgctg cggcagcaga gccgtgattg tcaggccagt
     4441 cggtgcactt tcgataaaca ggtgcccgcg caagccccgc acgcgctcgc gcatcagcga
     4501 cagaccaatg ccactgctgt ctgctgtgtt ggcaaaacca tttccattgt cggcaatcgt
     4561 cagttcgagg ttcttgtcaa cgagccgtgc ccgcacggtg atcctggttg cacccgagtg
     4621 cttcagcgaa ttggtgactg attcctgcac aacgcggtag caggcaatgc tccagttttc
     4681 agggaaaaag ccctcaagtg cctcgagatc ggtttcgatt ttgaccttag aaagcgaagc
     4741 cgcggattgt agcatatctt caagggccag gacgagtcct actttcttca tgtggctggg
     4801 cgtcaggcgg cgcgaaattt cccgcgcctt tttcagcgtg tcggaaatcg gttcgagcag
     4861 caggcgttca ttttcaggcg tgcgcatgcc ctggtcgccg agaaacttct gtaagttgag
     4921 tttcgtcaga acgagactct gtccaaggac atcgtgcagt tcactggaga tttccgcgcg
     4981 ttccatctca gaaatatcga taagtcgctg cgatagctcg agaattcgct gctcctgctg
     5041 tttacgcagc gtaatatccg cgtgcgtacc aaccatgcgc agtggtttgc tgccgctgcc
     5101 ccattcgaca atcttgccgc gcgccagaat ccatatccac cggccgtccc ggtggcgcat
     5161 acggaattct gcctcgtaac cttgcgtagg atcgccaaaa caacccgaaa gttttgcctg
     5221 gacgccggga atatcttctg catgcaaaag tccgctgaag ccctcgtaac taggccgcac
     5281 ttcagtgaca gcataaccga gcataccgca ccagcgctca tcaaagaatg cctcgccgga
     5341 cacaatgtcc cagtcccaga gacccaggtc tccaccgatg agcgcaagcg caagacgctg
     5401 ctcactttgt ttcagctgta ttgcagcgtt tttcgcctcg gtaatgtcct ggaacatacc
     5461 tgtcagtcgg gtgacccggt tgccttcgaa taccggataa cccgaggtat gtacccagag
     5521 tctattaccc ctggcagtga taaagcgcag gtccaggtcg taaggttcgc cggtctcgat
     5581 cgtgcgctgt aaggctgcga taagcagtgg ccggtcttcg ggatggtaaa actctatagc
     5641 tgcctcgcgc gcatagctcg tgccgggctg cacttcatga atccggtaga cttcatccgt
     5701 ccaggtagtc tggccggttt caacatccag ctcccacgcc cccatgcgcg caatcctctg
     5761 tgcctcggtg agcagacgcg aaagccggtt gagctccgcc tcagcctctt tagtcaccgt
     5821 aatatcgagt attgtaccgg tgtatcgaag cggggttcct tgcgcgtctc tttcgacgac
     5881 ctttccgcgc gaaagcaccc agactacacg gccgctcctg tgtcgtagcc gatgctgcgt
     5941 atctatccgg ggattctttc ccgaaaaatg ctcattcgat atacgtacgg cctcttcgcg
     6001 gtcttcgggc acgatcattt gccacgggtc ggcaaagaca ttgtaaaatt cttcaaccgt
     6061 gtatcccagc atctctgcaa accgttcgtt tacatacaac ctgcgttcag taatgcgata
     6121 atcgagcaga ccaagctgcg ctccttcaag cgccatttcc atgcgatcgc gggtttcttc
     6181 cagctcacgc tctgcgataa acttttcggt aatgtcggtc tgaatggcga caaagtgtgt
     6241 tacctcattt gcgtcgtttc gcaccggtgc aatacgcaac tgcgaaataa attgccggcc
     6301 gtctttacgg taattgcgca aaatgtattc gccgccttcg cgccgcttca gtgcagcacg
     6361 caggcgaatc aggtcgggtt ggtcatgatc atcaccatgg agaaaccggc agttccggcc
     6421 gatgatatct tctgcaccgt agcccgtgat agttgtaaag gcacggttga tgtacgtcac
     6481 cggcatatca ggcatacggg catcggaaat aataacgccg ctgagcgagc tattgatggc
     6541 ttcatcctgc aaccgcaggc gctgtagctg ctgcagatta cgctcgcgtt gtaaatacgc
     6601 ctcagtgatc tcgagaaagc tggcgacagc gccgatgagc ctgccacttg ccgcccaaat
     6661 gggtgaacag ctgaccgaaa gccatttggt gctgccgtcg gggaatacaa tgccatgttc
     6721 aaaaccgctg acgctttggc ccgcggtcag cgccaggctc agtggcagcg tttcaggggg
     6781 cagcggttca aaattgtgat cgatttgttt ccagtagtca gcctcctgga aataacgacc
     6841 tgtaacttcg tcgtgtttga gccggagtat gcgtgcggcg ctctcgttcg caaccgtgat
     6901 ttcacctttt gtatcgacga cgattatccc gttgatctgg ctgcggataa tggagtcgag
     6961 gtatgcctca cgctcctgca actgtccttc cagttcgaat cgctcggtaa tatcctggca
     7021 cgtgccaaac gccctaacgg gctttcccgc ggcgttgcgt tccacgcgcc agcgctcctg
     7081 cacatgcttt agccggcctg cagcagtgat gatgcgatgc gtaattgcat gttcaccttc
     7141 gctcgtgaat gacgacttaa aagaatcatc gaccagctgc ttatcgtccg gatgaacata
     7201 atcgaaaaaa ctatggtggg ttggcgcgaa agaatcagca tcaaggtcaa aaatgcggta
     7261 agtttcgtcc gaccagttta ccaccagcgt ctcgaggtcc gtttcccagt taccaagcct
     7321 ggcaacctgc tgtgcagccc tgagtctgcg ctcactctct tccagcgcct gggctgcctg
     7381 ccgtgcctgc tgccgcacgc gtgcttcccg gacttcgcgt tcgactgcag gcccgagtcg
     7441 cttgagcgac gacttcatga tgtagtcggc agcccccgca cgcatgagtt ccactgccac
     7501 accttcgcca attgcctgag aaaccaggat gaatggtata tccttgccac tttcgcgcaa
     7561 aatttccagc gcagttatgg cattaaattg cggcatgcgg aagtctgaaa tgatcacatc
     7621 ccatgaattt tgcagggcag cttttagggc acgtgaggtg tccacgcgca gcgtccgtaa
     7681 ctcgtagccg cgcgacttta gttcgtactc gacgagagct gcgtcgctct ctgagtcttc
     7741 aacgataagt acagagagct catgcagaat tgcctggcta ctggtcatgg tgtttcgccg
     7801 cccctcggca aggtgaaata gattgttgta cccactcccg gttgcgattg ggcccagatc
     7861 ttgccgccat ggcggtgcac gatacgttgc gctgtagcca ggccgattcc cgttccggga
     7921 aactcacctt cggcatgcag gcgctggaat ggagcaaaaa gcttatcagc atattgcata
     7981 tcaaagccca ctccattgtc gcgcacagcg cacagaaaat gctgccgttc cgcagcgcaa
     8041 ctaatttcta tacgaatcgg gtcttttccc tggctgaatt ttaccgcatt ttccagcagg
     8101 ttgcgaatca gaatacgcaa tagttcaggg tctgcataaa caatagagtt ttccggcagt
     8161 gcaagctcaa tcaccttgtc gctgttttct tcgcgtaact gctgaacaat ttcctgggcc
     8221 ttttgtgtca ggcttgcttc tacgggctgt atattcaacc gggcaacgcg cgaaagtgcc
     8281 agcatcgcct cattcatgcg cttcatcgca ccgatttcat tgcgcacata gaccagcatg
     8341 cccttgcctt tctcacccag cgccgaccga aattcgtggt ccagaatatt tagccagctt
     8401 tccgccctcg tcaggggagc tttcaggtca tgggagagcg aataactgaa ggcttcaagt
     8461 tccgcattaa tttcctgcag ctcacgtgtc cgctgtgcaa cagtctcttc aagcgttgcc
     8521 gcaagtccgg agatttcgcg ttttgcattc accagatctg taagctccac aagataacct
     8581 gcatacattc cgatctcagt catcggcacc acagtactgg taaaccatcg caccttctcg
     8641 gaaaactgcc tgccaaaaat gaaatccctg acaggtctct tttctcttac gctcacactc
     8701 aacggcatat cttcgagaga aatgcgattg ccggattcat cgataaaatg ccaatctgca
     8761 acatcatcca tcgagactgt tgactcaatt tcggattgcc gctttacctc tgggttcagc
     8821 gagattggcg cccctgcaga attgaatagc gtaatgccga tcggtgcgct ctcgaaaata
     8881 ttgcggaatt tctgctcgct ttctcgcagc gctgtttcag attcaataac gctggtaata
     8941 tccgttgaaa tacacacgac gagtccaaca tctgccaagt agcgcgtagt catcgaaacc
     9001 cagcgcaagt cttctttttc cctgtcgcga aatccgaaga cggcactgta atcctggtca
     9061 aacctcggca aggctagcgg cgcaggaaca tcgccgcgcg ggtacggttg accgtcagga
     9121 tagaagaaca tcctttcagc gattgctttc ttcaagtctg catcattcgc cacccgggca
     9181 atacgttcta ctgcagggtt tactttaata agctcgcgcc ggtgatttag gactgcaatg
     9241 gctacaggtg atgcgtcaaa aattgcgtcg agcagcgcgg cattctgtaa agactcttct
     9301 gccttcttga cttcagcgtc gatattcttg agagtaccaa tgatgcgcgt cgcctcgcca
     9361 gattcatccc gctcaacgac aataccgcgg tcctttaccc agaggtaatt tccgaattta
     9421 ttgcgcagac gtatccgcat atcaaatgcg ggaatatctc cacgccggta actttcgagc
     9481 atggcatctg aggcacgcag gtcatccgga tgtacatgtg cttcccatgc caaaggatca
     9541 ttttgtaatt caccctgctc gtagcccagc atcgcttccc aatggcgaaa aaaacggact
     9601 gtgcctgagg gcacatggta atcccatacg gcatcgacag tcgcctcgag cgcaaaatcg
     9661 agcagcatct ggttttcgcg cagggattgt tcggcaaagc tccgccagcg ttcagcttct
     9721 atgcggtcaa gcgcggcact gagatcggtg ccaagctggt caaagagacc aattatgtcc
     9781 gcagtgaaaa aaccctcttc ggctgcgtag aggttaagac ttcccattct aacgcccgaa
     9841 agcctgaacg taatagccgc atacgagtgc aggccatgtt tttgcctgcg ctcgctccac
     9901 tggtccgcag gcaagcgatc agggtaattc acgacaacat catgcgattc ccgcattgcg
     9961 gtgcctgtag gtccgcggcc ttctgccagg cggggcgaca tagaaacacg cagatcgtca
    10021 agatatgcgg ttaccgaacc ggctgccgcc gcaatgcgta ccagcatgga gtcgtcttca
    10081 ggcaggccaa tccatgcaag tttgatatag ttgtttgtaa cgcacaaccg tacgatcgcg
    10141 ctaaacaaat cgctctcggt gcttgtctgc gaaattatcc ggcgacagtt gctgagcagc
    10201 tcatacatga acgtgaggcg ctgcagcttt tccgcggctt tcttactctc cgtaatgtca
    10261 atcatcaccc cacgtaacca acgtgcatta ccgtcttctg ctgccaccga gacgatatca
    10321 cgcagccata cgatatggcc atctttcgca atcaggcgat attcaaaatc atgcggcgcc
    10381 agctcgcgac tctttgcctg acaatactcg ggaacccacg taacgtcatc gggatgcatc
    10441 gtccgaatcc agaagcccgg ggttaaccat tcttctactg agtagccgag cagcctgatg
    10501 gccttaccgc tgacaaaggt aaaatttact gttctggcat cggcctccca cactataccg
    10561 tcgaccgaat caacaatacc tgtcgtgcgc agctgatcag cctgctgctc acggcgcaac
    10621 ctgtccaatt gcatcgcaag aaaacccagc gaaggaacga tgctccacaa aaccagcggg
    10681 ccatacacaa gaagcggagt tggagcaggt gggagtttcg acggttctgc aagcggccat
    10741 gcagatgcgg cagcggcgaa ccgcgtaaaa tgggcgaccc cgaggataat gaagcccgta
    10801 gccacggccc cggcaattcg ccagtgctga gcgagcgccg caagtaccaa cactcgcgca
    10861 acatgcaccg aaaccaggcc aaggataatg ccactcagag caacgcgtgc tggcgcgtgc
    10921 ggcaacaacc acgaaaaata tccaagccct gccgtggcca gtccgatcag cacgaatatc
    10981 catctcgtct gctggtgaaa agacaggact ccattgtagg cacgtaccag aaaaaccgat
    11041 gaagccacga ggagtataga gccaggcatt gctgcgtata gctcaggcat actattcttc
    11101 agagcaatgg aaaagaagcc ggcagcctgc agcagcaggt accatgccca ggcaatcatg
    11161 ggccggtgag ggcaaacccg cgataccgcg agaaacagga accagaggga taatagcagc
    11221 gagatcaccg cacaaaagat catggcctgc gatgaggata tttctgccat tatccggacc
    11281 taataactca tgatttcttt ttacgggcaa acattgtcac gttagctgcg ttgagctcat
    11341 aataacgcgc cagcccaaat tcgcgcaaga catagtctgt caggtatccg ctaagcgatt
    11401 ctgctgaacc gagaatcagc acaccatcgc tcttgagcga tgcctgcatt ttttcaaaaa
    11461 gctgtttgcg ggtatcctgg gcaaaatagt atgctacgtt gcgacaaaaa ataatatcaa
    11521 acttgtcagg ggctggctgc gtgatgaggt tatgcgtgcg aaagtctata gacggcagca
    11581 aggtacggtt gatcatcgct cccccctcaa catcggagaa atacttgcgc ttgaatttct
    11641 caggcagtcc gcgcgagagt tcaaagttcg tgtacctgcc cgctgccgcg cgttcgactg
    11701 attcctgtga aatgtcggtc gcaataatct gcgctttttt cacgagctgc ggatgaaact
    11761 cggcgagaat aattgcgatc gaataggatt cctgtcccgt cgaacaggcc gcagaccaga
    11821 gttttaacgg agggtactgc agttcgttgc ctggtaatgc gttcctgtct ttccactccg
    11881 gcaagatctg ttctgcgagg gcatcaaaaa tactctcgtc gcggaaaaaa cgcgtctcgt
    11941 gcgtggtgat cttttcaatc acccggccga gcaatttatt gtcagtaccc ttctctatgg
    12001 cagaaagtaa ctggtcaaaa tcactgattc cgtgttcttt catcacctcg ggaaaacggt
    12061 gttcaaacag gtaatatttt tcgggctcta tgtgtaatcc agtcaggcgt ttgatcgccg
    12121 ccgcaatccc gggaaaggct ttatctgaca tcagcgcagg tactccgtaa ttttagccgc
    12181 aagcccttct acattgtgcg attcactgac gattccgtcg cgcaccggct ttgccggcat
    12241 gccgaatata acgcagtctg cagcttcctg ggccataagg taagccccgc gggcagcaag
    12301 attcttcatg ccaagatagc catcttcgcc catacctgtc ataatgactg ccagtgtgtc
    12361 gctggcaaaa aattcagctg ctgagttaaa gagaatattt gccgagggtt tacagagttc
    12421 acccggatta ctctcttcaa ttcggataac agctcctgtg ccacgcggct ccagcttcat
    12481 ctgcttgccg cccggcgcga tatagacggt ggcaggttcg gcgggcacac cattcgctgc
    12541 ttcgacaatt ttcagccggc tctcttcgtt caggctctcc gcaagctgcg cggtaaagag
    12601 cggcggcata tgctgcacga ccaagatgct gcccctgatg tcgggcggaa taaggcgaaa
    12661 gaggcggcgc aacgcgggag gacctcccgt tgatataccg atcacacagt agcgaaaact
    12721 cttacgacct tttacagtcg gtcgtggcga cggtggcggg ctaactaccg gggtaggagc
    12781 ctgtgaatgt tcgtgcctct gggtaaatgc acgaattcgt tcaacgatgc ggttcctgag
    12841 ctcagcggcg acattgccac cgagagcacc cgctggcttg gtaataaagt cgtctgcccc
    12901 gagctcaaga gcctgcagag tcagcctggc accttttgtc gtgtgcgagc tgaacatgat
    12961 gatacgactc tgcggagaga gcttcttgat ctcgggaata gtcgcaagac catcgagctc
    13021 cggcatttca tagtccagga gcacaatctc tggcttatag tggtttaccc tgggcaacgc
    13081 taggcgaccg ttcgaggcaa ctgaaacgac ctcgagatca ggttctgtcg ccagtgtctg
    13141 ccgcaggaag agccgataga cctctgaatc gtcgacaatc agaacctggg tcttcattac
    13201 cggagaaaac ctgggaggct acttgatgac cttaaattcc atgaccacgc taccgaggaa
    13261 gtcagagagt cttttgaggc ccgaagaaag ctcagagatc ttgtgcgcgt ctttggcact
    13321 gtcagtgaca gcctggctga tgcccgaaat attccggtta atttcagctg acgcttccga
    13381 gatctggtta acgttgctgg caatttcttt tgtcgtcatc gactgctctt cgacagccga
    13441 agcgatgctg gcattgcctt cgttcacgcg attgatgata cctgcgatgt tgccaattgc
    13501 ctgtatagtc tggtcggtac ttttctgaat cgcctgaatt tgcttcttga tatcttctga
    13561 agaggccgct gtctgccgcg caagctcttt cacttctgaa gcaacgacgg cgaagccctt
    13621 gcctgcttcg cccgcaccag ccgcttcaat cgaagcattg agggcgagca tattcgtctg
    13681 ctcggcaatt tttgaaatga tttcaattac cttgccgatg ctgtcagcgc cggtgccgag
    13741 ttcacgcacc aggccctgtg catcctgcgc tatcgaagcc gcttctttgg aaacacgagc
    13801 tccctctgat gccttggttg cgacctcgcc aatcgaaata gacatttctt caaccgcgct
    13861 cgagacaacc tgcaggttct ggttcatctg gttggccgcc gatgaaatgc tgcttgtttg
    13921 ctgcgaaatt tcttcaatac ctgtcgagag gttctggctt gaatctgcca gggtcttggc
    13981 acctgagttc aggtctttgc ccgtttcagt catctggaaa atcagaccag cgacgttcgt
    14041 gttcatgatc tgtatggact cgagcaggcg accggtttca ctttcgtcac ggacatcgat
    14101 aggcttggta aaatcaccct cacccatctc tttcatcgcc tggttcgctt cgcctacctg
    14161 tttcagaacc acatagcgcg tccaaagcag aactggcagt ttcagagcgc caatggtcac
    14221 acc
//
LOCUS       DAOQJF010000037        13990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2115_length_13990_cov_5.160818, whole genome shotgun sequence.
ACCESSION   DAOQJF010000037 DAOQJF010000000
VERSION     DAOQJF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13990
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2115_length_13990_cov_5.160818"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLF85_03430"
     CDS             <1..955
                     /locus_tag="PLF85_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNE18740.1"
                     /translation="LVHPDYLRFDFSHPKAMSAEELSAVENAVNEAIRANLAVDTAEL
                     PKAEAEKRGAVMTFGEKYGDVVRVVEMGDVSKEFCGGTHVSRTGEIGGFIIQKESSPG
                     AGNRRIEAFAGNAALAALDARLQEIAQALEPAAGLPEATRNHFAGELAALHAEPRGVG
                     LISVTWRKALDLLTRIAAAQQEARKQRAKEERAGSGSDHEVLATELLSGKMTLPGGTA
                     IAHSIVEGRNVGDLKSVADKAREKDPAVVYVIFSRSGEEKLFVLGTTQAYAAERKLQL
                     NALLKSAPAELGVTGGGKPELVQGKLRGDENALMEYIRSAV"
     gene            957..1334
                     /gene="acpS"
                     /locus_tag="PLF85_03435"
     CDS             957..1334
                     /gene="acpS"
                     /locus_tag="PLF85_03435"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNE18741.1"
                     /translation="MVVGLGIDICENDRISEMLGKYSERFLRRVFTDEEIAYCMKKKD
                     PIPHLAARFAVKEAFIKALGMPRDLTISYRDVGLRGSEGKKDIHVTGKLAQMLETKRA
                     TSILFSISHARDYSNACVVLEQI"
     gene            1334..2359
                     /gene="ruvB"
                     /locus_tag="PLF85_03440"
     CDS             1334..2359
                     /gene="ruvB"
                     /locus_tag="PLF85_03440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNE18742.1"
                     /translation="MVPPVDPYLNPQPNEQESQVRPQRLSEFIGQRDVRENLASFLKA
                     AALRGEVLDHVLLSGPPGLGKTTLARIIAQETGGGFSLLTAPNIKRPGDLAKILSGLS
                     KGDVLFIDEIHRLPAPAEELLYGAMEDRTIDISVGDAMLANSVQITLPPFTLVGATTR
                     PGDLTAPLRTRFGITFHLEFYNDDDIAQITRRAAQIWKIRCDDDALREISRRARSTPR
                     IALHLLRRVWDYALAESNDLERPVDLAAATSAFARMHIDAEGTTKLDRQFMLSIAQHY
                     DGGPVGLKPLAAILAEDLTTLEDYVEPYLVRRAFVRRTPRGRMLTREAYQHLGIKPGQ
                     QAGLFEP"
     gene            2397..2792
                     /locus_tag="PLF85_03445"
     CDS             2397..2792
                     /locus_tag="PLF85_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18743.1"
                     /translation="MFKPLCKTICLLLLTAQVVSAAPEINPGFTGDRATGETESAFRR
                     GEVIFFISYPFTFLGSLIVYNVAASGIRTLDTGRSDFVASGGFYALVATTAAFLSFGI
                     AMNDYYAIRTEARAEAGQPTSYLSLDYRF"
     gene            complement(2802..4487)
                     /locus_tag="PLF85_03450"
     CDS             complement(2802..4487)
                     /locus_tag="PLF85_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNE18744.1"
                     /translation="MRRAYYEIRRMLAVNSIIVDLLIDVFISYLYRPRGLRPRSFTRR
                     AIAGFVRRIFRRPKNFARFPVQFRRTLERLGPTYVKLGQILSLRDDMLPERITLELRK
                     LQSQVPPISYAEAKSVIEQEFNTPIRHIFAEFHETPVASASLAQAHMAKLRSGQKVVV
                     KVQRPGITRLISDDINVMRRLAAIMEMIPKLRDYRPAHFVEEFAKYTMRELDFAQEGK
                     HADIFRENFKDWDDIIFPRIYWDYTSRRVLTMEFIEGIKPDDREKLKRLGVNGPRLAA
                     RGAQAVLKMLYIDGFFHGDPHPGNMFIVEKNKICLIDLGMIGSFTPETRNNMFLYYYF
                     TVIREFDHATSYLVKLTEPGRKADVAGFRAELAEQIKRWSGAAAFKEYSLGRLIFDTM
                     NIGAKHRLYFHGDLVLSSKCIITIEAVGAILDPKMDLAKVSRPMMEQIFAEQMSFARY
                     GKSILRALPDYLEYAEKIPDQILKTIGMISSGKFQIEVTRPPEKESRERSNHFSTMGL
                     VSLCLAAVFLVRDDFSSAQWTLPLVGSVSALAIIAAISGLFFFTLARRTAPEN"
     gene            4595..5110
                     /locus_tag="PLF85_03455"
     CDS             4595..5110
                     /locus_tag="PLF85_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18745.1"
                     /translation="MRRYLVRHENQTLPFVVADDAVYTAEFPKAEARKPYTWNLASAS
                     QQVAQVEIEGLSHTAHYTVSGNVVFLELEGFFYRLPYTNLRKAQDAAGAAQAVRAEIP
                     GRIVKVLVKPGDAVTQNQPLIVQEAMKMEITLRAPANLVVASVQVAEGAQVEAEAVLV
                     TFENPDSGKRS"
     gene            5112..5579
                     /locus_tag="PLF85_03460"
     CDS             5112..5579
                     /locus_tag="PLF85_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE18746.1"
                     /translation="MAIPNLLASKQGIRHRIAGIYARGGKILLVKHRKAGREYYLLPG
                     GGQETGESATEALAREWKEELNLTVKVGKFLFCGESVPAEQRRTQVFQMVFQIDSIEG
                     NIEVHHEGALAGYDWVPINRIDEIAFFPDCLPQVKSFCRGEEFVTYQRYRWLT"
     gene            5600..7675
                     /locus_tag="PLF85_03465"
     CDS             5600..7675
                     /locus_tag="PLF85_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18747.1"
                     /translation="MIRRYFVSLAAILLATLAFFAENHLVKSRSAELTERRFDTLEEN
                     MRLIDRHVREKNDHGEYFRYLGARPGKDPDLAELNPAPRSVATAVLLDRDFRSLARSG
                     RDLTAEELSFLRAGRRSAVTKQGILRTYLLTDFSARGIAYLALAINPGSPDSNAALYA
                     ATLDFRIVTGLDSETFGREENLLLGALLSRQAAAREVFRLHGTSYLSVRRYLLQENMV
                     LFQLTALPPLYTFFSLYLLVAVTLLAAVWLLRGFQESRFTRRELSERILHSYSRAIDT
                     HTSALGELSRLAQHDGLIEEKLVVGDTRQADIEARIRAEREAQQQKERRPNPIVIDIM
                     PEERQFRFMNPARMVTPSSGVAQLGAREQKLRERAFSDELKSLMATMGEQPAEAPAPA
                     ATDGQLSESIRQFESRYHFPEIDQYLYYLNELYFDEVTGEELAQAMRVAGDAVQSHEF
                     AILLYDASAAAFRCGFVQGVPAELVRTLYLLPKDSIIPNDFADYGYVETTANLRKNSF
                     FAKRFPPGFSENLKGIHVFPLAESFLRARILFFDTARGGALSDPETVTTVRSYLRQVA
                     PALHMFFSETADTGGNPQDLAGWAVRELRECMVLAAPEHPLLVSQYVFESSLVADQQL
                     SLVREMTRILNDGEKVLVLSPSRILVAHGVGSGRAIESLVGAQGKKFIIKESEFGKTS
                     RNLYTFIEF"
     gene            complement(7715..10192)
                     /locus_tag="PLF85_03470"
     CDS             complement(7715..10192)
                     /locus_tag="PLF85_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNE18748.1"
                     /translation="MVKVVPGLFPAFFLFTLPLFAQALTISGRTFNESTGEPLEFGTV
                     IVPEAKFKSRINPDGTYAAAVPGPGEYTIQLSAPGLKTLTEKVRIDANLKRDFRLSLP
                     IVRTQTVKLRAERDIQKLGRNTLSVEQLKETPATFGDAINALATLPGVIRPGGFFGPL
                     VIRGADDKGNRYFIDDIPVPNPQHFGGLQSIISNDIMREVDLYSSAFPSQFGGAVGAI
                     IDIQTVDEVKEFGGVVDVSLISSNFLLKNKWGRSMGGEVRITPAAQTAEQGPNPLGVI
                     PFQSKKSGPTGYWITAGRIGYLTLLVPPIYKLITGNTIDRLPEYYDYQLKGKLFTDDA
                     GKHSLTALFFGSYDTLKFTRNLSAEEKEKRKQEGDDPVLANFNINNDVSSHSQGLYYE
                     YIPSSKMTNKLIAFNSYTYSYFRADLGSGSSIGATDLKVYPNIFGLKDKFRLDWADYA
                     TLRLAAEYNLFYFKSSGQTQQQTGALPSTGQPDLGNSALFQTVPVNFDDQNHILSAYA
                     ENKFQFGGLRFVPGVRTDFLARTKNVTLDPRALISYEFPTETTISVAGGQYQSYAQTN
                     TFYFNRVFNYQPQVVVADYLKPERSFHRTVGVEQKFSPFVFKVEGFMNDFDRLLEANG
                     TTVAQATGQYFSNSGELRARGVEVFLRKDKEDKANEFYGWLSYTYTQSERRRNGNWSR
                     FEFEQPHSIKVVAGYRWGANTIGAQFQLFAGFPYTPIIGASCTPGYDCSNGSTNVRYS
                     PTYSGDLYSERYPMSHRLDIRYTRKTTYKWGYFSWYIEVINIYNYVAPNQQRWNYNQP
                     FSASNPVVKVPDGAITIIPYFGLEWRF"
     gene            complement(10257..12743)
                     /locus_tag="PLF85_03475"
     CDS             complement(10257..12743)
                     /locus_tag="PLF85_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18749.1"
                     /translation="MQTIAERSGANQQVPTGQTIAAKGQLIRSLVILQKGQATVHSVE
                     TGPVGQPILRPLYAVNAPAVLAGAALFSGQRSNYQVVTSAQSEISVYPSNRDYLLKLI
                     AAKPNIGVLALKSIYKDLTDLEAKLDQAEQTWTQLESYELGFSIALSAILPEKFTGET
                     KNEAGFADPLLPLARQQVSDFTERGGNIVKPLNVNFLRSARNQIETVAEKKTESNTVG
                     LIRRLVSLPQEVLGAMAARDPQLLYIAAEAVGELAGSIGTEIERRLAEMQQFLQRMLI
                     GDYAWLAKFSLQAELMESNSNTASREDIIASSIFFAEGFGALRDKLLSFAEIAYPPYQ
                     VQALQKIQKVAAQKEQQAKPATADASQATPISGDLDEFIGAVDKILQWTETPADKSTQ
                     FKKDWDTLKKFKNPLDASDDDVRKLRRRVNMQYWEIFERGILKYLRKRADLPRYMRLF
                     FDYGFMDETQLDAEHLLKLSQASYGKGDPWLPIYTITEWLAAIYDRKVTTSISEMGLT
                     FFEMLRQDPSNRDKKWRKETDLPPEVNSPEARVKFEIKEMLQMNCRLCSGNISSHLNI
                     LTRHQITQPLEKVIITKDTIVQDIRKILHTDFGAFHREVLYTNEAMNIQREFIQTQVT
                     PNIVLVPSIGTVIQFWQDREGNDRMSRGRLIAPIMATADLQMMLLRAIGTYRWEICKT
                     TMGPDWNNISVSSLTADYTDYVQFFYKSKELSEEIKEKIAEDMKRFRDDRARFVNDYT
                     VYMRFESEGSQRMNRVARKIFTKHIPFAKEIRERLLKLPSYTEVVQKSINIRKKKANE
                     LTPRYTKYERENKTLPKELVETRRYYNMEY"
     gene            complement(12774..13604)
                     /locus_tag="PLF85_03480"
     CDS             complement(12774..13604)
                     /locus_tag="PLF85_03480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HNE18750.1"
                     /translation="MLHPSKLLKELGISPLKQLGQNFQVDVHSVENAAAQIPAGAVIL
                     EIGPGLGAWTEVFLGRGHKMILVEKDRTLAARLKDKFAGSEQVTVHEGDVLEFDLESA
                     EFAEVTAAVGNLPFYVTSEILLRLIVDCPRLTHGLFGIQLEVAERIASGKGSSLAIAL
                     GAQGKLFIAGKISRNSFYPIPNVDAAILGFERSATANRPHLRALLKAAFWGKRKTLAS
                     ALRKNPFWPEQAETVSWPERLMHAPPALQPLLTQRADELSVTDFQRLYDYLESGEYLT
                     "
     gene            complement(13698..>13990)
                     /locus_tag="PLF85_03485"
     CDS             complement(13698..>13990)
                     /locus_tag="PLF85_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE18751.1"
                     /translation="GNDENEEMVFVDTLESPEALNPDTIVEREEIKNMIVECIKKLPD
                     REKKVIVLYYYEDLTLKEIGEVLEVTESRVSQLHTKAIMRLRGRLSQMKSLM"
BASE COUNT         2968 a         3715 c         4068 g         3239 t
ORIGIN      
        1 tcttgtgcat cccgattacc tgcgttttga cttttcgcat cccaaggcaa tgagtgccga
       61 agagttgagt gccgttgaga atgctgtcaa cgaagcgatc cgtgcgaacc tggcagtaga
      121 caccgccgag ttgcccaaag ccgaggccga aaagcgtggc gccgttatga cctttggcga
      181 aaaatacggc gacgtggtac gcgtcgtcga gatgggcgat gtttccaaag aattctgcgg
      241 tggtacacac gttagccgaa caggcgagat tggcggcttc attatccaga aagagtcgag
      301 cccgggggct ggcaaccgcc gcatcgaggc gttcgccggc aacgcggcgt tggcggcact
      361 cgacgcccgc ctgcaggaaa ttgcgcaggc gcttgaacca gctgcgggcc tgcccgaagc
      421 cacacgcaat cactttgccg gtgaactcgc agccctgcat gcagaaccac gcggcgtggg
      481 attgatcagc gttacgtggc gtaaggcgct tgatctgctt acccgcattg cagctgcgca
      541 acaggaagcg cggaagcagc gtgccaaaga agagcgcgca ggctcaggca gtgaccatga
      601 ggtgcttgcg accgaactcc tgtcgggaaa gatgaccctg cctggcggaa cagccattgc
      661 acactcgatc gtggaagggc gcaacgtcgg cgatctgaag agtgttgctg ataaagcgcg
      721 cgaaaaagac ccggcagttg tctacgtgat attttcacgg agcggtgaag agaagctgtt
      781 tgtgctggga accacgcagg cttacgcggc agagcggaag ttacagctca atgcgctgct
      841 caagtcagca ccggcagaac tgggtgtgac tggcgggggc aagcccgaat tggtgcaggg
      901 caagttgcgc ggcgacgaaa acgcgctaat ggaatacatc cgttcggcgg tctgaaatgg
      961 tggttggcct gggcatcgat atttgcgaga acgatcgcat ctcagagatg ctcgggaaat
     1021 atagtgagcg ttttctccgg cgggttttca ccgacgaaga gattgcttac tgcatgaaga
     1081 agaaagatcc tattccccac ctggcggcgc gtttcgcggt taaagaggct tttatcaagg
     1141 cactgggtat gccgcgcgac ctgacaatct cgtaccgtga tgtgggactg cggggcagtg
     1201 aaggcaagaa agatattcat gtcaccggta agctggcgca gatgctggag accaaacgcg
     1261 caacgtccat tctgttcagc atttcgcatg cgcgcgatta ttctaatgcc tgcgtggtgc
     1321 ttgagcagat ctgatggtcc cacccgtaga cccttacctc aacccgcagc cgaatgagca
     1381 ggaatcgcag gtcaggccgc agcggctttc ggagtttatc ggccagcgag atgtccgcga
     1441 aaaccttgcc tcgtttctga aggccgcagc tctccgcggc gaggtgctcg accacgtgct
     1501 gctttcgggg ccacccggct taggtaagac cacgctggcg cgtattatcg cgcaggaaac
     1561 gggcggcggc ttctcgctgc tcacagcgcc caacatcaag cgtcccggag acctggcaaa
     1621 aattctcagt gggctcagca aaggcgacgt gctctttatc gacgaaattc atcgcctgcc
     1681 ggcacctgct gaagaacttt tgtacggtgc gatggaagac cggacgatcg atatttcggt
     1741 gggcgatgcc atgctggcga attccgtaca gatcactctg ccgcctttta ccctcgtcgg
     1801 ggcgacgacg cgcccgggag acctgacggc gcctctgcgg acccggttcg gtataacttt
     1861 tcacctggaa ttctataacg atgacgacat cgcgcagatc acacgccgtg ccgcgcagat
     1921 ctggaagatc cgctgcgacg atgatgcgct gcgcgaaatc agtcgccgag cccgcagcac
     1981 tccgcgtatt gcgctacacc tcttgcgccg tgtctgggat tacgcgttgg ccgagagcaa
     2041 cgacctggaa cgcccggtag atctcgcggc cgcgacttcg gcatttgcgc gcatgcacat
     2101 tgacgccgaa ggaacaacca agctcgaccg ccagttcatg ctgagtatcg cgcagcacta
     2161 cgacggcggc ccggtaggtt taaagcctct cgccgctatc ctggccgaag acctgacaac
     2221 cctggaagac tatgtcgaac cctacctcgt tcgccgcgcg ttcgtgcgcc gaaccccgcg
     2281 cgggcgtatg cttacgcgcg aggcatacca gcacctcggg ataaagccgg gccagcaggc
     2341 cgggcttttt gaaccctgat tgccgccgca ggtaaatggg aaaagatgcc ttaaccatgt
     2401 tcaagccgct ttgcaagacc atttgcctgc tgctactgac ggcacaggtg gtttcagcag
     2461 cgcccgagat taaccccggg ttcacgggtg accgggcaac cggcgaaacg gaatctgcgt
     2521 tccgacgcgg cgaggtaatt tttttcatct cgtatccgtt taccttcctg ggtagcctga
     2581 ttgtttataa cgtggcagca tcgggcatac gcaccctcga tacagggcgg agtgatttcg
     2641 tagcttcggg cggcttctat gcgctggtcg caactaccgc agctttctta agctttggca
     2701 tagccatgaa cgactattac gcgatccgca cggaagcccg tgcagaggca ggccagccta
     2761 cctcgtacct gtcgctcgat taccgtttct gaataatcgt ttcagttctc aggcgcagtg
     2821 cgccgcgcga gagtaaagaa gaagaggccg ctgattgccg caattattgc cagggctgaa
     2881 acactgccga ccagaggtaa tgtccactgt gctgacgaga agtcgtcacg caccaggaat
     2941 actgcagcca ggcagagcga aaccagtccc atcgtgctga aatgattgct gcgttcgcgg
     3001 ctctcttttt ccggcggccg ggtaacttca atctggaact tgcctgaaga gatcatgccg
     3061 atcgtcttca gaatctggtc agggattttt tccgcatatt cgagatagtc aggcagcgcc
     3121 ctgaggatcg atttgccata acgggcaaat gacatttgct cggcaaagat ctgttccatc
     3181 atcgggcgcg aaacctttgc gagatccatt ttgggatcaa ggatagctcc caccgcttcg
     3241 atggtaatga tgcacttcga tgagagtacc aggtcgccat ggaagtagag gcggtgcttg
     3301 gcgccgatat tcatcgtgtc gaagatcagg cggccgaggg agtattcttt gaatgccgca
     3361 gcgcctgacc agcgcttgat ttgttcggcg agttcggcgc ggaaaccggc aacgtcagcc
     3421 ttgcgacctg gctctgtcag cttcacgagg tagctcgtcg catggtcgaa ttcacgtatg
     3481 acggtaaagt agtagtagag gaacatgttg ttgcgtgttt caggagtgaa cgagccgatc
     3541 atgccaaggt cgatgaggca tatcttgttc ttttctacga tgaacatgtt acccgggtgc
     3601 gggtcgccgt ggaagaatcc gtcgatgtag agcatcttga gcactgcctg cgccccgcgc
     3661 gctgcaaggc gcggaccatt cacgccgagg cgcttgagtt tttcgcggtc atcgggtttg
     3721 atgccttcga tgaactccat ggtcagcacg cgccgcgagg tgtaatccca gtagatgcgg
     3781 ggaaatatga tatcgtccca gtctttgaag ttttcgcgga agatgtctgc gtgtttacct
     3841 tcctgcgcaa agtcaagctc gcgcattgtg tacttggcaa attcttcaac aaagtgtgca
     3901 ggccggtagt cgcgcaattt ggggatcatc tccatgatcg cggcgaggcg acgcatgacg
     3961 ttgatgtcat cgctgatcag ccgcgtgata ccggggcgct gtaccttgac cacgaccttc
     4021 tggccgctgc gcagttttgc catgtgtgcc tgtgccagtg aagccgaggc aaccggcgtt
     4081 tcatggaatt cggcgaagat gtgccggata ggcgtgttga attcctgttc gataacactt
     4141 ttggcctcgg catacgagat cggtgggacc tggctctgca gcttgcgcaa ctcgagcgtg
     4201 atgcgctcgg gcagcatgtc gtcacgcaac gaaagtatct gccccagctt gacgtacgtt
     4261 ggtccgaggc gttccagagt acggcggaac tgtacgggaa agcgtgcaaa attcttcggc
     4321 cgccggaaaa tgcggcgcac gaacccggcg atggcgcgcc gggtgaacga ccgcggccga
     4381 agcccccggg ggcggtagag gtatgatatg aagacgtcga tcagcaggtc gacaatgatt
     4441 gaattgaccg cgagcattcg gcggatttca taataggcgc ggcgcatgga atgggaaagt
     4501 tcatcgctta cagccgtgtc aagattttat tgaaaaggct gccgatctgc acgcttttta
     4561 tttgccaagg tgtaggcaat ggttgggata ccccatgcgc cgctacctgg tacggcatga
     4621 gaaccaaact ctaccgtttg tcgttgctga tgatgccgtc tatacggccg agtttcctaa
     4681 ggctgaagcc aggaaacctt atacgtggaa tctggcctcc gcatcgcaac aggttgcgca
     4741 ggttgagatc gaaggtctta gtcacaccgc gcactatacc gtctccggta atgtcgtgtt
     4801 cctcgagctt gaaggatttt tctaccggct accttacacg aatctgcgca aggcacagga
     4861 tgccgcaggc gctgcgcagg ccgtgcgggc agagataccg ggacgaattg tgaaggtgct
     4921 cgtcaagccg ggcgacgcgg tgacgcagaa ccagccgctt attgtgcagg aagccatgaa
     4981 gatggagatt acgctgcgcg cccccgccaa tctcgtggtg gcaagcgtac aggttgcaga
     5041 gggagcgcag gttgaagccg aggcagtact tgtaactttt gaaaatcccg atagcgggaa
     5101 acggagttaa tttggcgatt cccaacctgc tggcttcgaa acagggtatt cgccaccgca
     5161 tcgcgggtat ctacgcgcgt gggggaaaga ttctgctggt aaagcaccgc aaggccggtc
     5221 gcgagtatta tcttctgccc ggcggtgggc aagaaaccgg tgaatcggcg acagaagcgc
     5281 tcgcccgcga atggaaagaa gagctcaacc tcaccgtgaa ggtggggaaa tttctttttt
     5341 gtggcgagtc ggtgccggcg gagcagcggc gcacccaggt gttccagatg gttttccaga
     5401 ttgatagtat cgaaggtaat attgaagttc accatgaggg agccctcgcc ggttatgact
     5461 gggtcccgat aaaccggata gatgaaattg cctttttccc cgactgcctt ccgcaggtaa
     5521 aatccttctg ccgcggagaa gaattcgtga cataccagcg ttaccgctgg ctgacttgag
     5581 atacgaccgg taaaagcgca tgatccgcag gtattttgtc tcgctggccg ccatcctgct
     5641 cgcgacgctc gctttcttcg ccgagaacca cctcgtaaag tcgcgctctg ctgaactcac
     5701 ggagcgccgg tttgatacgc ttgaagagaa catgaggctg atcgaccgcc atgtcaggga
     5761 aaaaaacgat catggtgaat acttcaggta tctcggtgca cgaccgggta aagatccaga
     5821 cctggccgaa ctgaatccag caccgcgcag cgttgcgact gccgtattgc tcgaccgcga
     5881 tttccggtca ttggcaaggt cagggcgtga cctgacagct gaagaacttt cctttctgcg
     5941 tgccggccgg cgcagtgcgg tcacgaaaca gggaatactg cgtacctact tacttacaga
     6001 tttttctgcg cgcggcatcg cctacctggc gctggcgata aatcccggtt cgccggatag
     6061 taacgccgcc ctctatgctg caacgctgga tttcaggatt gtcactggcc tcgacagtga
     6121 aacgttcggc cgcgaagaaa acctgttact gggtgcgctc ttatccaggc aggctgcggc
     6181 gcgagaggta ttccggttac acggaacaag ttacctgtcc gtgcggcgct acctgctgca
     6241 ggaaaacatg gtgctgttcc agttaactgc cttgccaccg ctttacactt tcttcagcct
     6301 gtacctgctc gttgcggtca ccctgctggc agcggtctgg ttgctgcgcg gtttccagga
     6361 aagccggttt acccggcgcg aactctccga gagaattctg cacagttata gtcgcgcgat
     6421 tgacacgcat acctcggcgc tcggtgagct gtcgcgactc gcacagcacg atgggctcat
     6481 cgaagaaaaa cttgtcgtcg gtgatacgcg gcaggcagac attgaagcgc gcattcgggc
     6541 cgaacgcgaa gcgcaacagc agaaagagcg caggccgaat ccgatagtca ttgatatcat
     6601 gccagaagag cggcaattcc gcttcatgaa cccggcgcga atggtgaccc cgtcaagcgg
     6661 cgtggcacag ctgggcgccc gggaacaaaa actgcgcgag cgggcattca gcgacgagct
     6721 gaagtctctg atggcgacga tgggcgaaca acctgcagaa gctcccgcac ctgcggcaac
     6781 cgacgggcag ctgtcagaaa gtatcaggca gtttgagagt cgttaccatt ttccggaaat
     6841 cgatcagtac ctgtattacc tcaatgaact gtatttcgac gaggtaacag gcgaagagct
     6901 tgcgcaggcc atgcgggttg caggcgatgc ggttcagtca cacgagttcg ccattctgct
     6961 ctacgatgct tcagcggcag cttttcgctg cggttttgtc cagggcgtgc cggcggagct
     7021 cgtgcggaca ctgtacctgt tgccgaaaga cagcatcata ccgaatgact tcgccgatta
     7081 cggctacgta gagactacag ctaacctgcg gaagaattcg ttcttcgcca agcggtttcc
     7141 gcctgggttt tcagaaaacc tgaagggaat tcatgtcttc ccgcttgcgg aaagtttcct
     7201 gcgggcgcgc atcctgttct ttgatactgc ccgtgggggt gccctcagcg atcccgaaac
     7261 ggttaccaca gtgaggtctt acctgaggca ggttgcccct gcgctacaca tgttcttttc
     7321 cgagacagca gacacaggtg gtaatccgca ggatctcgcc gggtgggcag tgcgcgaact
     7381 gcgcgaatgt atggtgcttg ctgcaccaga gcatccgctg ctggtctcgc agtatgtttt
     7441 tgagagctcg ctggttgcag accagcagct cagccttgtg cgcgaaatga ccaggatact
     7501 gaatgatggc gaaaaggtcc ttgtgctgag tccatcacgc atactcgttg cgcatggtgt
     7561 tggatcgggc agggcaattg aatcccttgt gggcgcgcag gggaaaaaat tcatcatcaa
     7621 agaaagcgag ttcggtaaga ccagccgaaa tctctatacg ttcatcgaat tctgaaatcc
     7681 ccctgcacca gagcggtcca gggggaaacg attttcagaa acgccactcg agaccaaagt
     7741 aagggataat cgtgatggct ccgtccggaa ccttcacgac ggggtttgat gcggagaaag
     7801 gctggttgta attccatcgc tgctggttcg gtgcaacgta gttgtagatg ttaataactt
     7861 cgatatacca gctgaagtaa ccccacttgt aggtcgtttt gcgggtataa cgaatgtcaa
     7921 gccggtgcga catagggtaa cgttcgctgt acaggtcacc cgagtaagtc ggcgaatagc
     7981 ggacgttggt cgagccgttg ctgcagtcgt agcctggcgt acacgaggcc ccgataattg
     8041 gcgtgtaagg aaaaccggcg aacagctgga attgcgcgcc gatcgtgttg gccccccagc
     8101 ggtagccggc gacaactttg attgagtgcg gctgctcaaa ctcaaaccgg ctccagtttc
     8161 cgttgcggcg ccgttcgctc tgtgtgtagg tatagctcag ccagccataa aattcgttcg
     8221 ctttgtcttc tttgtccttg cgcagaaaaa cctctacgcc ccgcgctcgc agctcgccgc
     8281 tattcgagaa atattgtccc gttgcctgcg ctacggttgt gccgtttgct tcgagcaggc
     8341 ggtcgaagtc gttcatgaaa ccttccactt taaacacaaa cgggctgaat ttctgctcca
     8401 cgccaacggt gcggtgaaac gaacgctcgg gtttcaggta atcggcgacc acgacctgcg
     8461 gctggtagtt gaagacccgg ttgaagtaga aggtgtttgt ttgagcatac gactggtatt
     8521 ggccgcccgc caccgagatg gtggtttcag ttgggaattc gtaactgatg agcgcgcgcg
     8581 ggtcgagggt aacattcttt gtccgtgcca gaaagtcagt ccgcacgcct ggcacaaagc
     8641 gtagaccgcc gaactggaat ttgttttcag cataggcaga gagtatatgg ttctggtcgt
     8701 cgaagttgac gggtacagtc tggaagaggg cgctgttacc aagatcaggc tgcccggtgc
     8761 tgggcaatgc cccggtttgc tgctgtgtct ggcctgacga tttaaagtag aacaggttgt
     8821 attcggccgc cagtcgcagc gtcgcgtagt cggcccaatc gagcctgaac ttgtctttga
     8881 ggccaaagat attcgggtag accttcaggt cggtcgcgcc gatcgaagag cccgagccca
     8941 ggtcggcgcg aaaataactg taggtatagc tgttgaaggc aatcagcttg ttcgtcatct
     9001 tcgacgaagg aatgtactcg taatacaacc cctggctatg cgaagaaaca tcgttgttga
     9061 tgttgaagtt cgccagtacg ggatcatcgc cttcttgttt gcgtttttct ttttcttctg
     9121 cgctcaggtt gcgggtaaat ttcagcgtgt cgtagctgcc gaagaaaagc gccgtcagcg
     9181 aatgtttgcc ggcatcatcg gtaaaaagtt ttcccttgag ctggtagtcg tagtattcgg
     9241 gtagtcggtc gatcgtgttg ccagtgatca gtttatagat cggtggtacc agtagcgtca
     9301 ggtaaccgat acggccagca gtgatccagt agccggttgg gcccgatttt ttcgactgga
     9361 aggggatgac acccagcggg ttagggccct gttctgccgt ctgcgccgct ggggtgatgc
     9421 gcacttcgcc acccatgctg cggccccatt tattcttcag aaggaaattt gaagagatga
     9481 gtgagacatc cacgacacca ccaaattctt tgacctcgtc gaccgtctgg atgtcgatga
     9541 ttgctcccac agcgccacca aactgtgaag gaaacgccga cgaatacagg tcaacttcgc
     9601 gcatgatgtc gttggagata atcgactgta agcccccaaa gtgctgcggg ttgggcaccg
     9661 gaatatcatc aatgaaatag cggttgcctt tgtcgtcggc gccgcgtatg accagtggac
     9721 caaaaaatcc ccccggcctg atgacgcccg gtagcgtcgc gagcgcgttt attgcatcgc
     9781 cgaaagttgc aggggtttct ttgagttgtt cgaccgagag cgtgttgcgc cccagcttct
     9841 ggatatcccg ctctgcgcgc agcttcaccg tctgtgtgcg cacgatgggc aggctaaggc
     9901 gaaagtcgcg cttcaggttc gcgtcgatcc gcaccttttc cgtcagggtt ttgagacccg
     9961 gtgcagacag ctgtatggtg tattcacctg gtcccggaac tgccgcggcg taggtgccgt
    10021 cagggttgat gcggctcttg aatttggctt cgggtacgat cactgtacca aactcaaggg
    10081 gctcgcctgt actttcattg aaggtgcggc ccgaaatggt cagcgcctgc gcaaagaggg
    10141 ggagggtaaa cagaaaaaag gccggaaaca gcccaggaac taccttaacc atttggttaa
    10201 gatactataa tctgtccttg ccttctaaaa aaaatcaaaa accttggaca gatccgtcag
    10261 tactccatat tgtagtaccg gcgcgtttcc accagttctt ttggcagcgt cttgttctcg
    10321 cgctcgtact tggtgtaacg gggggtcagt tcgttggcct ttttcttgcg aatgttgatg
    10381 gatttctgca caacctcggt atagctgggc aatttcagca ggcgttcgcg aatttctttc
    10441 gcaaatggaa tgtgcttggt aaaaatcttc cgcgccaccc ggttcatgcg ttgtgaacct
    10501 tcagattcaa accgcatgta gactgtgtag tcgttcacaa aacgcgcccg gtcgtcccga
    10561 aagcgcttca tgtcttcggc gatcttttct tttatttctt cggaaagttc tttgcttttg
    10621 tagaagaact gcacgtagtc ggtatagtcc gcggtcagcg acgaaacaga aatattgttc
    10681 cagtcgggtc ccatggtagt cttgcagatt tcccagcggt aggtaccgat cgcccgcagc
    10741 agcatcatct gcaggtcggc ggttgccatg atgggtgcaa tcaggcggcc ccgtgacatg
    10801 cggtcattgc cttcgcggtc ctgccagaac tgtatgaccg taccaatcga tggtacgagc
    10861 actatattgg gagtaacctg cgtctggata aattcgcgct ggatattcat cgcttcgttc
    10921 gtatagagaa cctcgcggtg gaaagcgccg aagtctgtat gcagaatctt gcggatatcc
    10981 tgcacgatgg tgtctttcgt gatgattacc ttttcgagcg gttgcgtgat ctggtggcgt
    11041 gtcaggatat taaggtggct ggaaatatta cccgaacaga gacggcagtt catctgcagc
    11101 atctctttaa tttcaaactt cacgcgcgct tccggtgagt tgacctcggg cggcaggtcg
    11161 gtctctttac gccatttttt gtcgcggttc gacgggtcct ggcgcagcat ctcaaagaag
    11221 gtcagaccca tctcgctgat acttgtagtg acctttcggt cgtaaattgc cgcgagccat
    11281 tcggtaatcg tgtagatcgg cagccatgga tcgcctttac cgtaactggc ctgcgagagt
    11341 ttcagcaggt gttcggcatc gagctgggtt tcgtccatga aaccgtagtc aaagaacagc
    11401 cgcatgtagc gcggcaggtc agcgcgtttc ctgagatatt tcagaatgcc gcgttcgaaa
    11461 atttcccagt actgcatgtt tacccggcgg cgaagcttgc gcacatcatc atctgacgca
    11521 tcgagcgggt tcttgaattt cttgagggta tcccagtctt tcttgaactg ggtcgatttg
    11581 tctgccgggg tttcagtcca ctgcagtatc ttgtcaactg ccccgataaa ttcgtcaagg
    11641 tcgccggaaa tcggtgtcgc ctgtgaagca tctgcagtcg ccggctttgc ctgctgctcc
    11701 ttctgcgcgg cgactttctg gattttttgc agcgcctgta cctggtaggg agggtaggct
    11761 atttcggcga agctcagcag tttgtcgcgc agcgcgccga aaccctcggc aaaaaatatg
    11821 gaagaggcga taatatcttc acgagaggca gtgttcgaat tgctttccat cagttcggcc
    11881 tgaagcgaga acttggccag ccacgcatag tcgccgatca gcatgcgctg caggaattgc
    11941 tgcatttctg caaggcgcct ttcaatctct gtgccaatac tgcctgcgag ttcaccgacg
    12001 gcttcagctg cgatgtagag cagttgcggg tctctcgccg ccatagctcc caatacttcc
    12061 tgtggcaatg acaccagccg gcggatgagg ccgacagtat tcgattcggt ttttttctct
    12121 gctacggttt caatctggtt gcgcgccgag cgaaggaaat tgacgttcag gggtttaaca
    12181 atgttgccgc cgcgttctgt gaaatcgctg acctgctgcc gcgccagcgg taaaagaggg
    12241 tctgcaaatc ccgcttcgtt cttcgtctcg ccagtaaact tttctggtag aatcgcgctc
    12301 agggctatgg aaaaacccag ctcgtaactc tcaagctgcg tccacgtctg ctcagcctgg
    12361 tcgagcttcg cttccaggtc ggtaaggtct ttgtaaatgg acttcagcgc cagcacgcca
    12421 atattgggtt tggccgcgat cagtttcagc aggtagtcac ggttgctcgg gtagacgctg
    12481 atttcgctct gcgcagatgt aacaacctgg tagttgctgc gctggcccga gaacagggcg
    12541 gcgccggcga ggacggcagg agcattgact gcatagaggg gacggagtat cggctggcct
    12601 actggcccgg tttccacgct gtgcacggta gcctggccct tttgcagaat gaccagcgaa
    12661 cggatcagct ggccctttgc agcaatggtt tgtccggtcg gtacctgttg gttcgcgcca
    12721 ctgcgctcag cgattgtctg catcagatgc tccacatggg tgaaaacgtg gcatcacgtc
    12781 aagtattcgc ctgactcgag ataatcgtac aggcgctgga aatcggttac cgaaagttcg
    12841 tctgcgcgtt gcgtcagtaa cggctgcagc gctggcggtg cgtgcattag cctctcaggc
    12901 caggatacag tttctgcctg ctcaggccag aacggattct tgcgcagagc gcttgccagt
    12961 gttttgcgtt taccccagaa ggcagccttg agcagcgcgc gcaagtgggg acggtttgcc
    13021 gttgcactgc gctcgaaacc cagtatagcc gcatcgacat tgggtatcgg gtagaagctg
    13081 ttccgggaga ttttgccggc aatgaagagt tttccctgcg ccccaagggc gattgccagc
    13141 gacgaacctt taccgctcgc aatacgttct gcgacttcga gctgtatacc gaacaggccg
    13201 tgcgtgaggc gcgggcaatc gacgatcagg cgtagcagaa tttccgatgt gacgtaaaac
    13261 ggcaaattac ctaccgctgc ggtaacctcg gcaaattcag cggattccag gtcgaattcc
    13321 agtacgtcgc cttcgtgtac cgtcacctgt tcactgccag caaatttatc ctttagtcgg
    13381 gcagccagcg ttcggtcctt ttccaccagg atcatcttgt gcccgcggcc gagaaacact
    13441 tcggtccagg ctcccaggcc gggcccgatt tccaggatta ccgcacctgc aggaatctgc
    13501 gctgccgcgt tttccacgga atgaacgtcg acctggaaat tctgcccgag ctgtttgagc
    13561 ggagagatcc cgagttcttt gaggagtttg gaggggtgga gcatttgccg ctggcgtttc
    13621 gactccgctc aacgaccggc ggcaccggtt actgaggtgc cagagctgag cgcagtcgaa
    13681 gcgagtcgaa gtaaccgtta catcagactt ttcatctgcg acagtcgccc gcgcaggcgc
    13741 atgatcgctt tcgtgtgtag ctgtgagacg cgcgattcag tcacttcgag tacttcaccg
    13801 atttctttca gcgtcaggtc ttcgtagtag taaaggacga taactttttt ctcacgatcg
    13861 ggcagcttct tgatgcattc aacgatcatg ttcttgattt cttcgcgttc aacaatcgta
    13921 tcggggttga gcgcttctgg agattcgagc gtatcgacga agaccatttc ttcattctcg
    13981 tcattgccat
//
LOCUS       DAOQJF010000038        13780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2150_length_13780_cov_5.235701, whole genome shotgun sequence.
ACCESSION   DAOQJF010000038 DAOQJF010000000
VERSION     DAOQJF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2150_length_13780_cov_5.235701"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLF85_03490"
     CDS             <1..349
                     /locus_tag="PLF85_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE18752.1"
                     /translation="AIRDFDNPSARAQGYIFWLEPVAAPASISADWSASSNSQEGNGF
                     AGRTLPEILAEEESRAMAWAMREANGNQSDAALLLGIPRQTFNYRFRKLARQQASRGG
                     KKNAEQQQAGKTQ"
     gene            346..1287
                     /locus_tag="PLF85_03495"
     CDS             346..1287
                     /locus_tag="PLF85_03495"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18753.1"
                     /translation="MSQTEEKKYFPLKKRISHVFEFTFLMLLFAIINLFPFSMLHGVA
                     KFFRIMLSPLLASARRRIRSQVSEILKITDETELNRFVNNNLDHTIRSFFELMQSWKM
                     RKKSFLNKYVSFAEEAVAIAEDRSQGVVFAEGHFGNWEIPIPMYASIGFKVFYAAQKL
                     SNPYVDWVVKKIREGYGGGGAIYLKESEKYIPLLRRREPIGLVADQDAGNEGIFVDFM
                     GKKASTHAGPAVLAYLGKARLAIGFCIYQGKGRYVFYARTLYRFGGKSDFASSREAAE
                     KLTQLWVSEMEKEIRKYPEQYFWVHRRWKTRPPEERT"
     gene            1288..2478
                     /locus_tag="PLF85_03500"
     CDS             1288..2478
                     /locus_tag="PLF85_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18754.1"
                     /translation="MLRYLFGRLWKNPLSRYGMLAGILVISFALIFSARNQLATFFSR
                     LFTSPDWSDVARHSSRPFAFEENEKKAQSLIADAWIELYELAQEKNRGPQHKEGALAG
                     AVARRPYSAFLRRHAEEEIFTLLNALAKDCGSLLVTTGVSDSASAMGEVAEANTAAGR
                     AKQGMRDTMRDLLKIYAGRIEAALQKKPDYIPAIELAEEVFRGTCSMRDIATLYARAT
                     DYREYYLQKQLYDHDNGKLYNRNPEEFNARALEAYARDPYYRDLLARHFQATRFRTPY
                     HPAQLKNLRDAHARLRTKATTTALVRGLLAEARHSTPDIARKCHYELFALDYPGITDD
                     MDYLFALAETALRGGENTRAQNILSNALKSKNLKDAAQRREFERLAFGLELRLHESEN
                     LSRF"
     gene            2450..3622
                     /locus_tag="PLF85_03505"
     CDS             2450..3622
                     /locus_tag="PLF85_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE18755.1"
                     /translation="MKAKIYLDFNSTHPPDKNILAEARNFYLEHFANSSGLSLESQLV
                     NKRIEAAREEIAALFGIQPKQVIFTSCATESNNLLIREMYRRRSNAQFRAISSPFEHP
                     SVSEVLRTLDGAEVRILTATAAGELDKSAWETTDWQADLITAMAIQNESGIILPVEQL
                     LRQRRAETAFLCDGAQLLPKLCHNGPDILKPGFIRTLTAQGCFVTATGHKLGGGFGCG
                     LLLTPEKHLLNAEHPLLAGGNQELSLRAGSHNIEAIIALSLALRKKVEANDYTLWRQR
                     TIDFERLLTEKLSFIAGFDIIGSQADRAPGTTLLLLPEVPIDFLVMALDKEGITVSTG
                     TSCKSRSRTPSQALLAMGYSERHALSLIRLSYGQNFAGQEMATAAETIARAAAALL"
     gene            complement(3636..4913)
                     /locus_tag="PLF85_03510"
                     /pseudo
     CDS             complement(3636..4913)
                     /locus_tag="PLF85_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017860287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
     gene            5318..7273
                     /locus_tag="PLF85_03515"
     CDS             5318..7273
                     /locus_tag="PLF85_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18756.1"
                     /translation="MPALRARVLSISLISLLACSSATRREDASSLEALLSRAEELRQA
                     GNTDSAAYERLLFDLETLRTRDTTLCQDAGQREKAAATLAQYPALQCEVRITEPREKF
                     FTATGFASPLRLVAECPANAPFTFEADSKTLALSGDAMRFTKGYLNARIRVSSASPDT
                     AKNAIQLTARYYAQTFAERLAETCALRATQGISVFPAEYRIQAAPVVATSAYDSFRQN
                     FRKAEWESAAEDGETRSEAALSANGQFTLGGKHRGRRFDLLYGHPNGELPDGIGTSFT
                     TVRIDGTDFRFEQQKLKRSKRSDGALLAETQLPGTSITLAQIIQPEKSGERVRIRIAY
                     QIRNTGKKQHKVGIRLLLDTWAGKNDGVPFLLPAAGVEQLVHTERDLSPAESLLWHTF
                     DPAENGNAEAEPALEGFLTGKGLTPPDRLAIVNWPNAVNTVWDYATHDERRITGDSAA
                     AFWWYPAAIKPGQTQQVATEVGAHLRQREPAVFVTNAASGDVLVYLWHRNASETAERV
                     SYELRAEKGNFAFKAQPGEVNLEPGAVYAKASPAQILAEGETTIVIKEIVNGSAREYR
                     FLVQNLRQWKQMVSPVVAEPGTPLPVSYFDERELDLQARLKDSAGGLIGKTPLARKKI
                     DGGFEYTGMLTLPPGITHGRYSVEVVR"
     gene            7270..7725
                     /locus_tag="PLF85_03520"
     CDS             7270..7725
                     /locus_tag="PLF85_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE18757.1"
                     /translation="MKTCRAILQLLCPVLLAATGLWAEEVLSNRPVIIAPRRIEISPA
                     ADPSGNTTVARPPEQRGSLRLAFRRGSMRLTEQTRRALRALRGSKNLLKITGYGDAAR
                     PNEKLANLRARAVASYLEEYSGIRRVEISWQAEACHEAGIGATITEEKE"
     gene            7725..9836
                     /locus_tag="PLF85_03525"
     CDS             7725..9836
                     /locus_tag="PLF85_03525"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNE18758.1"
                     /translation="MHIFASIIYNLTLATALYAVVMAIIHLRKQGNGRYLAQARSALE
                     LTLIAVSICVALLLFMLVSADYSIVYVAEHVNNQLPVFYRMTSLWAGQAGSMLWWNFL
                     VVFFSWLAVKHIRRAAPALEAWMLIILMTLVAFFTAIGCFAPSSDPFRVFQEGEKLFA
                     HAEGRGLNPLLQHWAMIIHPPILYFGYVSFAVPFAIAMAALLVKPAGSTTEPQVAWVK
                     LVRRWTLFSWFFLGVGILLGGKWAYEELGWGGYWAWDPVENASLMPWLTATAFLHSII
                     VQERRGMLKVWNMVLVSISFIMTVVGTALTRSGVVQSVHAFAQSNLGWFFLGFVIVTV
                     IFCTWAIISRYDALKSAQPIESLLSREVGFLFNNVLLLASLFIVFFGTMYPTISESIS
                     GRRESVTAEWFNRFMGPMGILILFLTGLGPLLAWRVTTVRSLLRNARIPLAAGLVTAL
                     FAWLIAQRGNEWTASRAMAIMTFGISAFVIAGVLEEWLRAAASRWRYTGEGIFTAILL
                     VLFQNKRRFLGYMVHIALAILFSGFSGLAFATEGRLMLNAGEAQSFGKYTIHVEKYEE
                     KAMPEGAQIPLYGTRIVTIGVYRDNVLVGRDTTEIRTYPMYNFREGKFSDSQNTSEPA
                     IISTLLADVYLQLGGDEKGRLILQIWINPLVKWVWAGFAFFIAMGLLLLLPIGEKKFI
                     SIGSLRFSVLPVAAPRADNPG"
     gene            9921..12554
                     /gene="ppc"
                     /locus_tag="PLF85_03530"
     CDS             9921..12554
                     /gene="ppc"
                     /locus_tag="PLF85_03530"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNE18759.1"
                     /translation="MRVVKDSDLEDDIRYLHGILEEVIAKLEGEDFLARLRQITALSA
                     EYQAHHDETGFASLVQAISGSDALLATKLTRALSTYLTLVNIAEEHHKVRIENNAVAP
                     VLPIAPDLVLHEFRQQGISPEKLHETVASLRIELVLTAHPTEIMRRSLLSKYSNLARY
                     LELRDRDITDAHEVKVNEEAIRREIFGIWETDPIRKKKPTPTDEAYGGLLVFEQTLWT
                     EVPRFLRELSDDLLHHTGKPLPLASAPIRFGSWMGGDRDGNPNVTAAVSQRAVWLAQW
                     MAAVLYEKEINALRFELSLTHCDAVLRGLTQEAHEPYRVYLKKTLTRMTSTRERLEDL
                     LQQGTTQIIEYYRSTEELTQELMPVYESLVATGMQAIADGRLSDLLRRLAVFGLYLVK
                     LDIRQESGRHAAAISEITRELGLGDYSAWAEHEKIAFLCEELKQRRPLVPVDIECSAE
                     TREVLDTFLMLAKIRTESLGAYVISMAKSASDVLLVHLFQKINGRKRHLRVVPLFETV
                     EDLRNAPRILSNLLKIPEYRELIEDKQEIMIGYSDSSKDSGILTAAWQLYRCQEEIIA
                     VCAEAGVEVTLFHGRGGTIGRGGGPTQIAILSQPPGSVAGRIRVTEQGEMIQAKFGLP
                     TVARRTLAIYTAAVLRASLAPPAGPPPEYRRIMETLAEQAARDYRSLVYEDAKFIDYF
                     RSATPEQELGNLNIGSRPARRKAGGIESLRAIPWIFAWTQTRFLLPSWLGVGAALSAI
                     QDEGDLRLLECMRQDWYFFRSFIDLIEMVLVKSEPRIAQYYDAMLTDEALSSVGDRIH
                     ESLKRTIEMVMRLSQEKELVSYDTNLQREIRFRSLWLNPLNLLQVEFLRRLRQTPEDE
                     ALRRGLLLTINGIAAGLKNTG"
     gene            12594..13727
                     /locus_tag="PLF85_03535"
     CDS             12594..13727
                     /locus_tag="PLF85_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18760.1"
                     /translation="MNRLLRSAGIMLISAAAIVAAPKKKETKAPSADAARVAILLYSD
                     KTGTQNFEYMPGSLKEAITGSMHEKFEFNEVDTTKVDPIVSQVRSKNKGVIGAKEAAD
                     ICRQADIDILIYGDFTFNKDANEIEIHTNISLGSTDKFRLLPAVENRVDSTIFQAADK
                     VATDIVAEITKVALEQQQAKGKAAEADKNKKTQLDKTEKSKTWADINWTFALSMGPVY
                     PLVNRDNANVKLEPAANAYGMYRLKGNWHVGLFATFSGFRSNTSNSPYETNIEYIAST
                     ANLGYYFDLSARWRLTTMVGAGYYLGKYSVSSTCSSANTQCFAPNTVPETQIRNPFAL
                     ARTGIHFLIFSFLSAGIEGEYRMLYDSKPIHSVGGAASLTLTF"
BASE COUNT         3086 a         3921 c         3721 g         3052 t
ORIGIN      
        1 ggcgattcgc gattttgata atccatcggc gcgggcgcag ggttatatct tctggctgga
       61 gcccgttgca gcccccgcca gcatcagtgc agactggagc gccagcagta acagccaaga
      121 aggcaatggt tttgccggcc gcacattgcc cgaaattctt gctgaggaag aaagccgcgc
      181 gatggcgtgg gcgatgcgcg aggccaacgg caaccagtcc gatgcagccc tgcttttggg
      241 aataccgcga cagactttta attaccgttt ccgcaaactg gcacgccagc aggcctcgcg
      301 gggcggcaag aagaatgcag aacagcaaca agccggaaaa acacagtgag ccagacagaa
      361 gaaaagaaat acttccctct caaaaagcgc atatcgcatg tctttgagtt caccttcctg
      421 atgctgctct ttgccatcat caatctcttt ccattttcga tgctgcacgg cgtggcaaaa
      481 tttttccgta tcatgctttc gccgctgctc gcatcagcgc gcaggcgcat acgttcacag
      541 gtcagcgaaa ttctgaaaat cactgacgag acggagctca atcgatttgt caataacaac
      601 ctcgaccaca ctatccgcag tttttttgag cttatgcagt cgtggaagat gcggaaaaag
      661 tcgttcctga acaaatatgt ttcgttcgcc gaagaagctg tcgctatcgc agaagaccgt
      721 tcgcagggtg ttgtgtttgc cgaagggcac tttggcaact gggaaattcc catacccatg
      781 tacgcctcga ttggttttaa ggtcttttat gccgcgcaga aactctctaa cccctacgtc
      841 gactgggtgg taaaaaaaat acgcgaaggc tacggtggcg gcggcgctat ctacctcaaa
      901 gaatcagaga aatatattcc gctgctgcgc cgcagggaac ccattggcct tgtcgcagac
      961 caggatgcgg gtaacgaagg tatcttcgtg gacttcatgg gcaaaaaagc ttcgacgcat
     1021 gccggccctg cagtcctggc ttatctcggg aaagcccggc tcgccatcgg cttctgcatc
     1081 taccagggca aaggtcgcta cgttttttac gcgcgcacgc tctaccgctt cgggggtaaa
     1141 tcggattttg cctcttcgcg cgaagccgca gagaaactta cgcaactgtg ggtcagcgaa
     1201 atggaaaaag aaatacgcaa ataccccgag caatacttct gggtacaccg gcgctggaaa
     1261 acccggccgc ctgaagagag aacatagatg ctgcgctacc tgtttggccg gctttggaag
     1321 aacccgctct cgcgttacgg catgctggcg ggcatactcg tgatcagttt tgccctgatt
     1381 ttttctgccc gcaaccagct cgctaccttc ttttcccgtc tctttacgtc gcctgactgg
     1441 tctgacgttg cccgccactc gtcgcgtcct tttgcttttg aagagaacga aaagaaagcg
     1501 cagtcactca tcgccgatgc atggattgaa ctctatgaac tcgcgcagga gaaaaatcgc
     1561 ggtccgcagc acaaagaagg cgcgctggcg ggtgctgtcg ccaggcgccc ctactcggca
     1621 tttctgcgac gccacgccga agaagaaatc ttcacgctac tcaacgcgtt ggccaaagac
     1681 tgcggctcac tgcttgtgac aacaggtgtc agcgattctg cgagtgccat gggcgaagtg
     1741 gccgaagcga acacggcagc cggtcgcgca aaacaaggta tgcgcgacac aatgcgggac
     1801 ctgctgaaaa tttatgccgg caggatagaa gccgcgctgc agaaaaagcc tgactacatt
     1861 cccgcgattg aacttgcaga agaagttttc aggggcacct gctcgatgcg cgacattgcc
     1921 acactctatg cgcgcgcgac tgactaccgc gagtactacc tgcagaaaca gctctatgac
     1981 cacgacaacg gcaaactcta taaccgtaac cccgaagaat tcaacgcgcg agcactggaa
     2041 gcctatgcgc gcgaccccta ctaccgcgat ctgctggcgc ggcatttcca ggcaacccgc
     2101 ttccggactc cgtaccatcc tgcgcagctg aagaatctgc gggatgcgca cgcgcgcctg
     2161 agaacaaagg caacgactac agccctggtt cgcgggcttt tggccgaggc acggcatagc
     2221 accccagaca ttgcgcgcaa gtgccattac gaactttttg cactcgatta tccgggcatc
     2281 accgatgaca tggattatct tttcgcgctg gcagagactg cgctgcgcgg cggcgagaac
     2341 acgcgggcgc agaatatttt gtccaacgcg ctgaaatcaa aaaacctgaa agatgccgcg
     2401 cagcgccgcg aatttgagcg cctggcattc ggtctggagc tgcggctgca tgaaagcgaa
     2461 aatttatctc gattttaatt ccacgcaccc gcccgataag aacatactcg ccgaggcgcg
     2521 caacttctac ctcgagcact tcgcaaactc ttcgggactt tcgctcgaat cgcagctggt
     2581 caataagcgc attgaagcgg cacgtgaaga aattgcggca ctgttcggta tccaaccgaa
     2641 gcaggttatc ttcacctcgt gtgccaccga atcgaacaac ctgctcatac gcgaaatgta
     2701 ccggcgccgg tcgaacgcac agtttcgcgc gatcagctcg ccatttgaac acccttcggt
     2761 ttcagaagtc ttgcgcacgc tcgacggcgc agaagtcaga attctcactg cgaccgctgc
     2821 gggcgaactc gacaagagcg catgggagac caccgactgg caggcagacc tgatcacggc
     2881 gatggcgata cagaatgaaa gcggaattat tcttcctgtt gaacaactac tgcggcaaag
     2941 acgggctgaa acagcgtttc tctgcgacgg ggcacagctg ctacccaagt tatgccataa
     3001 tggccctgac atcctgaaac ccggttttat ccgcacactc accgcgcagg gatgtttcgt
     3061 tacggcgacg gggcacaagt tgggtggtgg ttttggttgc gggcttttac tcaccccgga
     3121 aaagcatctg ctcaacgcgg aacatccgct gctggcagga ggcaaccagg aattgagcct
     3181 gcgcgcgggc agtcataaca ttgaagccat tattgcgctt agcctggccc tcaggaagaa
     3241 agtggaggca aatgactata ctctctggcg gcaacgaacg atagattttg agaggttgct
     3301 tacagaaaaa ttgtctttca ttgccggatt tgacattatt ggcagccagg ctgaccgcgc
     3361 cccgggaacg actctactcc ttttgcccga agtaccaatt gattttctcg tcatggccct
     3421 cgataaagag ggaatcaccg tctctacagg gacttcctgt aaatcccgct cacgcactcc
     3481 ctctcaggcc ctgctcgcca tgggctacag tgaacgccat gcgctgagcc tgataaggct
     3541 gtcttacggc cagaatttcg ccgggcagga gatggctacg gccgccgaaa cgattgcgcg
     3601 cgctgcggca gccctgctct gaaatcctgc gctatctatt tggacgaacg cgcaagtttt
     3661 gtcgaaaggt atttttgtat ttcaggatcg ttatcctgcg tgagctgcca gaaagagaac
     3721 cctgcaagct tacttttctg cagtaatgac atcttctcag caaaagcagt acggtccatg
     3781 tagaacgcta cgcgtgtgca accgcccgag cggtaccaga gcgaagggtc ttcatagacc
     3841 ttatcttcgt ggatgtcgcg gaagcgcgtc aggttacgcc atgtacccga accttcagtt
     3901 gtgtcgagta tcttgctgat attggtgggc tgtatgttcc tgtgctcgcc tgacttgata
     3961 cgaaccacat cgctgtaata aactgctttt atcggagcgc ggcaattgag gccccagtca
     4021 taaccgtacg tcggtatggc catgtagagt ttttccgcgg ggatcttgtc gcctgcatat
     4081 tcgatgattt cttcgagcca tgtgcccggt gcctgcggac ctggccccgg gttacggtac
     4141 ttgcgcgggt gcagttcgta agccatgatc ttcaccatgt ctgcgtgctt acccaaaaat
     4201 ttatagtcgt gcgtcaacgg cccccgccag ttctcacgga aatcgagcgt gatcggttgt
     4261 ttaagtccgg cgcacgactt ctgctttaac gggcggcggg cagtcttttg cttcaaactc
     4321 ctggcggcgt atttcaggct ggttttgacc gcagattttg cagcggcgtc ggttattgag
     4381 ctcttggtgg caggcggagt ttcttcttcg tcatcttctt catcgtcatc gtcatttaca
     4441 ccgctgccga ccggtgtttt cggatgtacc gcaacagaaa ggagcttctt acgcgcgtgc
     4501 aactcgcctg agagcttctc aatgaactct tcaaattttt cttttttgtt gcaactcata
     4561 ccctcgtagt caatgtcgat gccatcgtaa ccgtaggttt cgacttcttt aacaatgatt
     4621 tcgatgtggc gatcgcgtat atcgtcgcga ccattcatgc caatgacttc gttaattttt
     4681 tctccggggt tttcccagcg aaaaatggtc ggtatgatac ggattcccgg atttttggcg
     4741 cgcaatgcag caacacgctc cagcttctgc gtatgacccc aagcagaaga aatttccggt
     4801 gggttattct gccttccctt gagcgagtag ataaaaggat gcagctcgtc atagagctcg
     4861 gcgtactgca gcatcacctt gaaatcagaa gaccaggtcg acgccttaaa cgaaggtgtc
     4921 gactgcggac gcgggctgct cacggctgcc ttatcaatat gcacctgcgg cgactgtttg
     4981 aagagccgca gcacgaacgg tgtggcaaca agcagagcga taccgcctat cgctgcggca
     5041 accagcagct tcttgcggcc cgaaggctgt ccttcctggc tttcttcggg cgccggttcc
     5101 ggctcagccg ggggattaac ctgctcttct tgcagaactt tcttcttttt cattatgcac
     5161 tcattatcgg ctgtccgcca agagggcagt atgcaataca aacatgatgt gtcattcctg
     5221 attcaatatt cgcccctgtc gccagggcca gtgcaacaag ggcgcattgg caggttatcc
     5281 tgattgtcag cgtgtgttgc agcacaagtt acgtgccatg cctgcgctgc gcgcccgcgt
     5341 tctgtcgatc agcctgattt ccctgcttgc ctgcagcagc gctacccggc gtgaagacgc
     5401 atcgagcctg gaagcactgc tttcccgggc ggaagagctg cgccaggcag gcaataccga
     5461 cagcgccgcg tacgagcgcc tgctgttcga cctcgaaact cttcgcacgc gcgatacaac
     5521 attgtgccag gacgctggcc aacgggaaaa ggctgccgcg acgctggcgc aatacccggc
     5581 gctgcaatgc gaagtacgca tcacagaacc acgagagaaa ttttttactg ctacgggttt
     5641 tgcttcaccc ctgcggctcg ttgccgagtg ccccgcgaat gctcccttta ccttcgaagc
     5701 agacagcaag actcttgctc tgagcggcga cgccatgcgt tttacaaagg gctacctcaa
     5761 cgcgcgtatc cgtgtatctt cagcgagccc tgataccgcc aaaaacgcga tccagctcac
     5821 cgcgcgctac tacgcgcaga catttgctga gcgcctggct gaaacctgcg cgctcagggc
     5881 cacccagggt atcagcgttt ttccggcgga gtaccgcata caggcagcgc cggttgtcgc
     5941 taccagcgca tatgactcat tccggcagaa cttccgcaaa gcagaatggg aaagcgcagc
     6001 tgaagacggt gagacgcgca gtgaagcggc gctttcggct aatggtcagt tcacacttgg
     6061 cggaaaacac cgtggccgcc gctttgacct gctctatggc caccccaatg gcgagctacc
     6121 cgatggcatc ggcacatcgt tcactacggt gcgcatcgat ggaacagatt ttcgttttga
     6181 gcagcaaaaa ctcaaacgct ccaaacgcag cgatggggca ctgcttgccg aaacacagtt
     6241 gccaggcacg agtatcaccc tggcacagat tattcagccc gaaaaatcag gcgaacgcgt
     6301 gcgcattcgt atagcctacc agattcgcaa taccggcaag aaacagcaca aagtcggcat
     6361 acggctgctg ctcgatacct gggcaggaaa aaatgacgga gtgccttttt tgctgcccgc
     6421 ggcaggcgtt gaacaactgg tacacaccga gcgggatctt tcccctgctg agtcactgct
     6481 ctggcatacg ttcgatcctg cagaaaacgg taacgcggag gctgagcccg cactcgaggg
     6541 atttctcacg ggcaaagggt taactccccc tgataggctg gcaattgtca actggccgaa
     6601 tgccgtaaac actgtctggg actatgcgac gcatgacgag cgccgtatca caggagattc
     6661 tgccgccgcg ttctggtggt acccggctgc gatcaagccg gggcagaccc aacaggtggc
     6721 taccgaagtt ggtgcacacc tgcggcaacg cgagcccgcg gtgttcgtga ccaatgcagc
     6781 ttcgggtgat gtgctggttt acctgtggca ccgcaatgca agtgaaacgg ctgagcgtgt
     6841 tagctatgaa ctcagagcag aaaaaggaaa tttcgcattt aaggcacaac cgggtgaagt
     6901 gaacctcgag cctggtgcag tctatgccaa agcaagtcct gcgcaaatac tggccgaagg
     6961 ggaaaccacc atcgtcatca aagaaatcgt caatggcagc gcgcgtgaat accggttcct
     7021 ggtgcagaat ctcaggcagt ggaagcagat ggtttcacct gtggtcgctg aacctggtac
     7081 cccactgccg gtcagctact tcgacgagcg cgagctcgac ctgcaagcgc gcctcaaaga
     7141 ctctgcgggg ggcctgatcg gcaaaacccc gctggcacgg aaaaagatcg atggcgggtt
     7201 tgagtatacg ggtatgctca cactgccacc aggcattacg cacgggcgct acagcgtaga
     7261 ggtagtgcgg tgaaaacctg tcgtgcaatc ttgcagctgc tctgccctgt tttgctggca
     7321 gcaacgggac tctgggctga agaagtactg agcaatcgcc cggtaattat cgcaccgcgg
     7381 cgcattgaaa tttcgccggc ggcggatccc tctggtaata cgacggtcgc aaggccaccg
     7441 gagcagcgag gctcgttgcg ccttgccttt aggcgaggtt caatgcgtct gaccgagcag
     7501 acacgcaggg cattgcgcgc actgcgcggt tcgaaaaatt tgctgaagat taccggttat
     7561 ggcgatgccg cccgcccgaa tgaaaagctg gcgaacctgc gagcccgcgc tgtcgcttcg
     7621 taccttgaag aatactcagg catacgccgt gtcgaaattt catggcaggc agaagcctgc
     7681 cacgaagctg gcatcggcgc aacaataaca gaggaaaaag aataatgcat atcttcgcat
     7741 ccatcattta caacctgacc ctggcaactg cgctctacgc agtggttatg gcgattattc
     7801 acctgagaaa acaaggcaac gggcgctacc tcgcgcaggc gcgttcggcg ctcgagctca
     7861 ccctcattgc tgtctcgatc tgtgttgccc ttctgctctt tatgctggtg agcgccgatt
     7921 attccattgt ctacgtcgcc gagcacgtca ataaccagtt gccggttttc taccgcatga
     7981 cctcgctttg ggcaggacaa gcaggttcga tgctgtggtg gaatttcctc gtggtctttt
     8041 tcagctggct cgcggtaaaa catatccgcc gcgctgcacc ggcgcttgaa gcatggatgc
     8101 tgatcatcct gatgactctc gtcgcgttct tcactgcgat tggctgcttc gcgccatcgt
     8161 ctgatccgtt ccgtgtattc caggaaggtg aaaaactttt cgcgcatgca gaaggccgtg
     8221 gattaaaccc cctgctgcag cattgggcga tgatcatcca tccgccgatt ctttattttg
     8281 gctatgtctc gttcgcggtg ccttttgcca tcgcgatggc tgcgttgctg gtaaagccgg
     8341 caggttcaac gacagaaccg caggttgcct gggtaaaact cgtccgccgc tggacgcttt
     8401 tcagctggtt ctttctcggt gttggtattc tgctcggcgg caaatgggca tacgaagagc
     8461 tgggttgggg cggctactgg gcatgggatc cggtggaaaa tgcatcgctc atgccctggc
     8521 tgaccgcgac ggcttttttg cactcgatta tcgtccagga acgccgtggc atgctcaagg
     8581 tctggaacat ggtattggtt tcgatctcgt tcatcatgac cgtcgtgggc acggcgctca
     8641 cgcgttcggg cgttgtccag tctgtgcatg catttgcgca gtcgaacctc ggctggttct
     8701 ttttgggatt tgtcatcgtc acggtaattt tctgcacctg ggcgattatc agccgttacg
     8761 acgcactaaa atctgcgcaa cccattgagt cactgctgag ccgtgaagtc ggtttcctct
     8821 ttaacaacgt attgctcctc gcatcactct tcatcgtttt ctttggtacg atgtacccga
     8881 cgatcagcga aagtatttca gggcggcgcg agtcggtgac cgccgaatgg ttcaaccgtt
     8941 tcatggggcc gatgggtatc ctgattttgt tcctgacagg gcttggcccc ctgctcgcct
     9001 ggcgcgttac cacggtgcgc tcgctgctgc gtaatgcgcg cattcccctt gcggcaggtc
     9061 tcgtaaccgc cctgttcgca tggctgatcg cacagcgcgg caacgagtgg accgcctcac
     9121 gcgccatggc aattatgact tttggcattt cggcctttgt cattgcaggt gttctcgaag
     9181 agtggctgcg cgctgcggcg tcgcgctggc gctataccgg tgaaggcatt tttaccgcga
     9241 tactgctcgt gctcttccag aataagcgcc ggttcctggg ctacatggta cacatcgctc
     9301 tcgcgattct tttctccggg ttttccggcc tcgcatttgc tactgagggt cgactcatgc
     9361 tcaacgccgg cgaggcccag tcttttggta agtatacgat acacgtagaa aagtatgaag
     9421 agaaagcgat gcccgaagga gcgcagattc ccctctacgg tacccgcatc gtaacgatcg
     9481 gcgtctaccg cgacaacgtt cttgtcggtc gcgacacgac agaaatccgc acgtacccta
     9541 tgtacaactt tagagaggga aagttttctg attcacagaa tacttctgaa ccggcgatta
     9601 tttcaaccct gctggctgac gtttacctgc aattgggggg cgacgaaaaa ggcaggctga
     9661 ttctgcagat ctggattaac cccctggtca aatgggtatg ggcgggtttt gcgttcttta
     9721 tcgcgatggg ccttctgctg ctcttgccaa tcggagaaaa gaagttcatt tcgatcgggt
     9781 cgctgcgctt ctctgtgctg ccggtggcag caccgcgcgc ggacaacccg ggctgacagt
     9841 tctgccaggc tcgctggtag cggtcacgga gcccggaatt tatgcttaac gcacgggctc
     9901 ggtctgcctg accgtccggc gtgcgcgtcg tgaaagattc agatcttgaa gatgatattc
     9961 gttacctgca cggcattctt gaagaggtta tcgcaaaact cgaaggtgaa gattttcttg
    10021 cgcggctgag acagataacc gcgctttctg ccgaatacca ggcgcaccac gacgaaacgg
    10081 gcttcgcgag ccttgtgcag gcgatttcgg gcagcgatgc gctgctcgcc accaagctta
    10141 cacgcgcgct tagcacctac ctgacgctcg tcaacatcgc ggaagaacac cacaaggtac
    10201 gcatcgagaa caatgcggtg gcgccggtac tgcccatagc accggacctg gtcctgcacg
    10261 aattcaggca gcagggtatt tcgccggaga agctccatga gacagtcgca tcactgcgca
    10321 tcgagctggt actgactgcg cacccgaccg agatcatgcg gcgcagcctg ctcagcaagt
    10381 actcaaacct ggcacgctac ctcgagctcc gcgaccgtga cattaccgat gcgcacgagg
    10441 taaaggtaaa cgaagaggca atacgacgcg agatttttgg catatgggag accgacccca
    10501 tacgcaagaa aaaacctact cccactgacg aagcttatgg cggcctcctg gtctttgaac
    10561 agacactctg gaccgaagtc ccccgctttc tgcgagaact ttctgacgac ctcttgcacc
    10621 acactggcaa accgttgcca ctcgcgtcag ccccgatacg gttcggttcg tggatgggcg
    10681 gcgatcgcga cggcaaccct aatgttaccg ccgccgtcag ccagcgcgca gtctggcttg
    10741 cacaatggat ggcagcagtg ctctatgaaa aagagatcaa cgcactgcgc tttgaacttt
    10801 cgctaacgca ttgcgacgcc gtgctgcgcg ggctcacgca ggaggcccat gaaccctacc
    10861 gcgtctattt gaagaagaca ctcacgcgca tgacctcaac ccgcgagcgg ctcgaagacc
    10921 tgctgcagca gggtacgacg cagatcatag aatactaccg cagcaccgaa gaactgacac
    10981 aagaactcat gcctgtctat gagtcactgg tcgccacagg catgcaggca atcgccgatg
    11041 gacggctaag tgacctgctg cgccgtctcg cggtgtttgg cctctatctt gtgaaactcg
    11101 atatccggca ggaatcgggc cgtcacgctg cggcaatttc cgaaattacc cgcgagttgg
    11161 ggctcggcga ctattcggca tgggccgagc acgaaaaaat tgccttctta tgcgaagagc
    11221 tcaagcagcg caggcctctc gtacccgtcg atatcgaatg ctcggctgaa acacgcgagg
    11281 tgctcgacac ctttctgatg ctggctaaaa tccgtaccga gtcattgggc gcctatgtca
    11341 tctcgatggc aaagagcgcc tccgacgttc ttctggtcca cctgttccag aagatcaatg
    11401 gtcgcaagcg gcacctgcgc gttgtgccgc tgttcgaaac cgtggaggac ttgcgcaacg
    11461 cgccgcgcat tctctctaac ctgctgaaaa tccccgaata ccgcgaactg atcgaagaca
    11521 agcaggaaat catgatcggt tattcggata gttccaagga ttcaggcatt ctcactgctg
    11581 cctggcagct ataccgctgc caggaagaaa tcatagctgt ctgcgctgaa gcgggcgtcg
    11641 aagtgacgct ctttcatggc cgcggcggta ctattggtcg cgggggaggt cccacccaga
    11701 tcgcaattct ctcacagccg cccggctccg tcgccggtcg tattcgcgtg accgaacagg
    11761 gtgaaatgat acaggcgaaa tttggtctgc ctaccgttgc ccgccgaacg ctcgcgatct
    11821 atacggccgc cgtgctgcgc gccagtctcg ctcccccggc aggtccaccg ccagaatacc
    11881 gcagaatcat ggaaactctc gccgagcaag cggcgcgcga ttaccgctcg ctggtctatg
    11941 aagatgccaa gttcatcgac tacttccgct ctgcgacacc cgagcaggag ctaggcaatc
    12001 tcaacatcgg ctcacgcccc gcgcgcagaa aagccggtgg cattgaaagc ctgcgcgcaa
    12061 tcccctggat tttcgcctgg acccaaacaa ggttcttgtt accttcgtgg ctcggtgtcg
    12121 gtgcagccct ttctgccata caagacgagg gtgatcttcg attgctcgaa tgtatgcgcc
    12181 aggactggta ctttttccgc tcgttcattg atctgatcga aatggtgctg gttaaatcag
    12241 agcctcgcat tgcacagtat tacgacgcca tgcttacaga cgaggccctc agcagcgtgg
    12301 gcgaccggat acatgaatct ttgaagcgca ccatagaaat ggtgatgcgc ctgtcacagg
    12361 aaaaggagct cgtctcatac gacaccaacc tgcagcgcga aatccgtttt cgctcgctat
    12421 ggctgaaccc gctcaactta ctgcaggtgg agtttctgcg caggctcagg cagacacccg
    12481 aagatgaggc tcttcgccgc ggcttgctgc tcaccatcaa cggaattgcc gccggtctaa
    12541 aaaataccgg ctaacttgtt gacattggcg gcgttttttc gtccaaatcc cgtatgaatc
    12601 gcctattacg gtctgccggc ataatgctta tttctgctgc cgccattgtc gcggcgccaa
    12661 agaaaaaaga aaccaaggcc ccgtcggccg atgcggcacg ggtcgccatt ttgctgtaca
    12721 gtgataagac cggaacccag aactttgagt atatgccggg ctcgctaaaa gaagcgatta
    12781 caggttcgat gcatgaaaaa ttcgagttca acgaagtcga cacgacaaag gtagatccca
    12841 tcgttagcca ggtccgcagc aaaaacaaag gtgtcatcgg cgccaaagaa gcggccgata
    12901 tctgccgcca ggccgatatc gacattttga tctatggaga cttcacgttt aacaaggacg
    12961 caaacgaaat tgaaattcac acgaatattt ccctcggctc gactgacaaa ttccgcctgt
    13021 tgcccgcggt cgaaaaccgc gtcgacagca cgatctttca ggcagccgac aaagtagcga
    13081 ccgacatcgt cgcagagatc accaaggtcg ctctcgaaca gcagcaggca aaaggtaaag
    13141 cggccgaagc agacaagaac aaaaagaccc agctggacaa gacagaaaag agcaaaacct
    13201 gggccgacat caactggacg tttgcgctaa gcatgggccc ggtatacccg ctggtaaacc
    13261 gcgacaatgc taacgttaaa ttagaaccgg cggccaatgc ctacggcatg taccggctta
    13321 aaggtaactg gcatgtcggt ctgttcgcca cattttcggg attccgctcg aatacaagca
    13381 attctccgta cgagacgaac atcgagtata tcgcgtcgac ggccaacttg ggctattact
    13441 ttgatctatc ggcgcgatgg cgactgacaa cgatggtcgg ggccggctac tacctcggca
    13501 aatatagcgt gagctcaacc tgctcttctg ccaacacgca gtgttttgcc cccaacaccg
    13561 tacccgagac gcaaatccgc aacccattcg cactggcgcg cacgggtatc catttcctga
    13621 tcttctcttt cctttctgcg ggtatcgagg gtgaataccg catgctctat gatagcaaac
    13681 cgatccacag cgtcggcggc gcagcgagcc tcaccttaac attctgaggg tggccatgct
    13741 cgcgagaatt ttcgggattc tactgctcgc gctggcactc
//
LOCUS       DAOQJF010000039        13775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2152_length_13775_cov_4.844388, whole genome shotgun sequence.
ACCESSION   DAOQJF010000039 DAOQJF010000000
VERSION     DAOQJF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_13775_cov_4.844388"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLF85_03540"
     CDS             <1..205
                     /locus_tag="PLF85_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18761.1"
                     /translation="MHVYEVAELVRDARFSAALAHLSETELAELHRDLAEAAGWIRRP
                     SRFRQAFQLSTAVFERRGIKVDF"
     gene            220..1539
                     /locus_tag="PLF85_03545"
     CDS             220..1539
                     /locus_tag="PLF85_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE18762.1"
                     /translation="MPMRRTIGLVTATSLVAGSMIGTGIFIKPAIMAQHGGSVAIVLL
                     AWLVAGLLSYAGALTYGELCARLPAAGGEYAILRESYGSFTAYMYGWMRFTIGAPGSA
                     ASYAVGAAAFLQVVAPYHGLGIKTWHVAAAFLTFFTLINCLTLFVSATVQVTLTVIKI
                     ASLVTIGFTLLWYAPASTAVAPPTQWPGMLSFSAALMAALWAYDGWNNLPMLGGEVSH
                     ARRNLPLALALGMGIVLATYMLVNAGYFRVLSFSAVVASNPSVREGAQPVAALAMHTV
                     ISDASVRVVAALMVISALGSLNGSLLSSARVPYAMATDGVFFPPLARLSRNHAIPVLS
                     TLSQGAIAILLAMSGTFDQLTDSVVFASWIFYALTAGAVFRLRRRADKSESIFHVPLY
                     PVLPALFIACSAAFIAYAIFAMPYLTGIGLLVIALGAPIYMLFRRRR"
     gene            complement(1760..1885)
                     /locus_tag="PLF85_03550"
     CDS             complement(1760..1885)
                     /locus_tag="PLF85_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18763.1"
                     /translation="MEIILLVIANFGPAGFQSIEIHNYDESVSSPKASMHTLAMT"
     gene            complement(1888..2571)
                     /locus_tag="PLF85_03555"
     CDS             complement(1888..2571)
                     /locus_tag="PLF85_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNE18764.1"
                     /translation="MRKYAILGLLPALLLAAACSTSKISIRKDALRQMQTIAVMPFSS
                     TVADAKVTRESTEIFRSAMVASGFKVLEREKIDKILKEKELAQTGLIENKALEAGQLL
                     GAEGTMLGEITAHEVHSEIVEAELPDEGPGKYDAKNDKGDGTYFRRGAKWFKKDKRDT
                     FQFQIVVRLVSNIDSQTVLTVQNEYPVRTFSGDSGGLRPANIDQFRAQVLAQMSKDIE
                     KAIREAKEK"
     gene            2727..4934
                     /locus_tag="PLF85_03560"
     CDS             2727..4934
                     /locus_tag="PLF85_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18765.1"
                     /translation="MQEGSPEYSQSAIVVQDRSSLTAPNSADMEVLAKLDEKLRFIEA
                     KKVSVNDPTYYLALDHISVANPTALEAAVKKVTDIANGGKNRVAYLYPMLVSHHSKPI
                     FQLFLFYPPKNKVGVRDAGPLRRAALAKSEPLMRSLLEAKRSKPTDIEEEDPGAVLVK
                     PDARGGKFYPDFFSLEYDFRRLLKKSYDKLQMPYIEIPDFFEDLRGFFERNSDLVDRI
                     GDRYHIVIDQLMLDDGGNYARVPEVIRHYRVLADGLEKFVLNVLHDRAKKGGASAYVA
                     QLDNYRATHVVTAQPGRKQSKEKVDVLMKLAAEYPFDRIQDRENNALREKLNETLSWL
                     GVLIEKKDRLVNRKQQQTVDNLLQKIENLIQSNTRQNLDLLQIDLYREITRTVKDDAQ
                     AQAMAADFEKRLMTRFGSYKVTAQDGTSLWYFLDQKYMSSVLENLSRLMVNDGGAAER
                     YEIATQIYEQLAKRKDPALDQGLSAEERINLSQMRAHNVNKTAEKKQLEHKANRYNIP
                     QGVLVSLISAILFAAAMFYSTQLFWLVFPLTLVSVIAFYRINFQSSPKTRSGEKESKS
                     LEAGADDSSSQMAKQMQEQANATRQKVATYGAAMVFPYMPKKFSERVLTEDEFRDKVR
                     LAAPEVKHQVPELGGLDGKALEQAVYEALREEAVCIVIPTDIVKKSLNKVPPALYIDY
                     KDFASDQTRNKIAEEYRAIFKPNLKDFEQDFYRYLINTLEVKYYTYLKKNLKK"
     gene            complement(4951..6252)
                     /locus_tag="PLF85_03565"
     CDS             complement(4951..6252)
                     /locus_tag="PLF85_03565"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNE18766.1"
                     /translation="MSEQEKITTSPFGRFLKMGALAGRVGFSLVGEKALGLFLSEDSR
                     EMRMAKAWAKNAEKIAETLGKLKGGAMKIGQMLSVQEALLPKELAFVLKTLQRDAPVV
                     ASGIMFRQLDSEIPHWRTLVKEIEQKPMASASIGQVYRAVLHDGTEVAVKIQYPGIDE
                     AIFSDLKNLRRLFELVLESMLAANLDHLFAEIEARLSEEIDYERELRRISEFRTFYAH
                     HPEFCIPEPIEELSSRRVLTTKMLKGLTIEEARHRSQHEKNTWGRLLFRSVTGQLLAL
                     RKLQSDPNPANYAFLDSGRIALYDFGSIKELPDWLHQGYMRTIDVCQAGRFDELTSIA
                     ASMQVHLKNGEPVPYKHVELFRSTLSPMFAVRPYKFRTETDPIKAIINYNRENMDELK
                     QITFPGDVLFVDRAVAGLIGNLTALGAEANWGDELLAALHP"
     gene            complement(6259..6717)
                     /locus_tag="PLF85_03570"
     CDS             complement(6259..6717)
                     /locus_tag="PLF85_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNE18767.1"
                     /translation="MILATLCYVQHEGKTLMMHRNKRPDDVHLGKYNGLGGKFEPGES
                     PEECVVREVFEESNLRIVPQLRGVMTFPQFAKGHDWYCFLYTAQNFTGEIKPDCPEGD
                     LVWVPDEQVLELPLWPGDRIFLGWLKQPKFFSAKFRYENGQLQGHEVIFY"
     gene            complement(6714..7271)
                     /gene="lptE"
                     /locus_tag="PLF85_03575"
     CDS             complement(6714..7271)
                     /gene="lptE"
                     /locus_tag="PLF85_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNE18768.1"
                     /translation="MRLRFLLPCITLASLVHCASLGRGNAEIESEALSESYRQFRGKT
                     RLLYIQNFDNRTYSPQLTGRLKEKLQIAFSRSASLTITPDKNKAELIMYGKILQYSEE
                     PGVYDRAAGPLTYNLAIVASFRLRARATTETEEAPYEQHTIRYMTAYSVGEPMFETRF
                     TAEDRMLEGLADRMAATAYEPGKTP"
     gene            complement(7268..8719)
                     /locus_tag="PLF85_03580"
     CDS             complement(7268..8719)
                     /locus_tag="PLF85_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNE18769.1"
                     /translation="MKPIKKILIANRGEIARRIMRTARTMGISCVAIYEQEDHDALYV
                     READAAYILHDGYLDQDRILEICAAEKVDAIHPGYGFLSENSAFVRRVEKAGITFIGP
                     SADAMDRMGEKTSARAEARAASVPLLPGEELEDGAVLDSTARRQLVEKIGVPVVLKAS
                     AGGGGKAQAIIEDISEIDAAFDKVLREAERLYRSRSLVAERYLAKARHIEVQVLGNAA
                     GKNFSLFDRDCSAQRNNQKIIEEAPAPGVPDKIRAELHASAIRLANHVGYSNAGTMEY
                     LYDSHRQEFYFLEMNTRLQVEHTVTEMITGLDLVREQILIAQGAITDYSAVTCTGHSL
                     QARVCAEKSDGTWQPSTGEILQYSEPALVRIDSGVAAGQKISGKYDNMIAKVIVHAPD
                     REAAMDQLHTALGHYVINGIHTNIPLLRQLLRDPVFREVRHYTRYLQKEFAPPQNNPA
                     LAAAIAAIALYQTELQENQERFGSLAGFTNTIQ"
     gene            complement(8722..9741)
                     /locus_tag="PLF85_03585"
     CDS             complement(8722..9741)
                     /locus_tag="PLF85_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE18770.1"
                     /translation="MLLAAEIFSVSEVWHGSSARRKDPVHPDLKLHHPKITPEFLSSF
                     KPTPLDFEMGWVIVDESARKYPDRITAADLRKTDPGKPEPLASDKRPRITNRGEFLWS
                     EGPNDLATGKNNEGLAFVLANDMDRAREIFEELRQNEPQFFPGRFNLGRVYLYFKQHR
                     EAIAEFEKCIALVPQYSKNYYYLGKSYELAAQRDAADYNYLRAYRYDIYNLESLVALG
                     DSLLEQKRTVEALNVFKHCLKQDSGFNDALIGMGKVAYTMKKHYDATLWFRSVNTRVA
                     YKKELHFYYGESAFYSQDYQTAARQYETMLNFPQDAIYSKVSLVRMRKRLEQTKRLLN
                     QTVDQ"
     gene            complement(9765..10517)
                     /locus_tag="PLF85_03590"
     CDS             complement(9765..10517)
                     /locus_tag="PLF85_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNE18771.1"
                     /translation="MSNRFFGLSYSMWTEQARWALDHHVIRYSYFEHVPMITTPILRL
                     AAGKLTGPVSAPILVANGRAFTDSWDIVAYADREGHREKLIPLDMVDEVRAWHDRCDI
                     ARKSNRIIATDATAHNEDAKRDSLPDFFPEFTKNVLTPLADLGVAYLGMKYDFRHAII
                     AEEKKKVREVLNQARKALGNKRFIFDNFTYADITIAATLQFVRPVGDHYIRLKPAQRE
                     VFTQKDLANEFADLLEWRDQLYKDKRGESHLL"
     gene            complement(10604..12133)
                     /locus_tag="PLF85_03595"
     CDS             complement(10604..12133)
                     /locus_tag="PLF85_03595"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNE18772.1"
                     /translation="MAQAPLLADTGQLAATETQATASHDSGHATVDPQKELGKQLSIW
                     TVIPFVIILLAIAVLPLLIPHLWESNLVKAVISALLALIMGAYLVSLGYLGIEVIVET
                     LKEYYAFITLLIALFTISGGIFIEGDLKATPLTNTIILGIGSVLASFIGTTGASVLLI
                     RPLIRTNKERHKTTHIFVFFILLVSNVGGVLLPVGDPPLFMGYLYGVPFFWTLIKLWP
                     IWLTAVAALLIIFFIWDNIAYKAEKRADLRSDDSHQTRIRVQGAHNFLLILGVLLAVV
                     FLKDYKTDHGTLAMGWLQQPVMMALAMLSFSLDYMDRTRKRKKHEEDLIQQIKTQGKV
                     DDKVLRSLSHYHPITPRDRNHFGFAPIIEVAALFIGIFITMIPALCILKARGAETGVS
                     QPWQFYWITGGLSSFLDNAPSYATFFALGQGLTSSMGAAAGDVVKATTGAISESILVG
                     ISVGAVFFGANTYIGNAPNFMVKAMCEESKIKMPSFFGYMAYTTLILVPILALIAAIY
                     I"
     gene            complement(12208..12918)
                     /locus_tag="PLF85_03600"
     CDS             complement(12208..12918)
                     /locus_tag="PLF85_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE18773.1"
                     /translation="MRKWAASKPKKKQSVASKAPKAAPLQVTPAPVAQHAEPSAVTSA
                     PAVQPAKKASAFKEILLALLLLIALAALFYFIVYRSGCATPKPETTAQTVEAPPAATP
                     QATPAPAPAAAEPAAPAASAELREFVAAQKLAFRAEKTELTPASVTVLDALAERLKKE
                     NARIRVTGHSCTLGPKSINQEISEKRAQVVKDALVSRGVAADRIETRGAADSEPVGDN
                     RTVAGRVASRRVTFSIIP"
     gene            complement(12895..13182)
                     /locus_tag="PLF85_03605"
                     /pseudo
     CDS             complement(12895..13182)
                     /locus_tag="PLF85_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     gene            complement(13232..>13775)
                     /locus_tag="PLF85_03610"
     CDS             complement(13232..>13775)
                     /locus_tag="PLF85_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE18774.1"
                     /translation="RHLADKYDQRWLYPAPNALELEERFDTAVGPHTRRFIYRKLFDA
                     RLSLPQLFSQPVIPPWQAALLPAIAPMLQAAMTSDMQINHDEAERSRQIFEGEFDFVA
                     GLLGDGRPFLCGDAISAADITFAALAAPVLLPDEYGVKLPDMRGLPATRQSHETVEHY
                     RRTPAGKFVLRLYREHRRPR"
BASE COUNT         2843 a         3743 c         3948 g         3241 t
ORIGIN      
        1 catgcatgtc tacgaggtgg cggagctcgt gcgggatgcg cggttttcgg ccgcgctggc
       61 tcatttgagc gaaaccgaac tggcagagct gcaccgcgac ctcgccgagg ctgcgggttg
      121 gatacgccga ccctcgcggt ttcggcaggc atttcagtta tcgacggcag tctttgaacg
      181 ccgcggaatc aaggtagatt tctgagagaa tcctcaccca tgcccatgcg gcgcacgatt
      241 ggtctcgtga ccgcgacaag cctcgttgcc ggctctatga tcggcacagg tatcttcatc
      301 aagccggcga tcatggcaca gcacggaggc agtgtagcaa tcgtgctgct ggcctggctc
      361 gttgccggcc tgctctcata tgccggcgct ttgacctatg gcgaactgtg cgcgcgcctg
      421 cctgcagcgg ggggggagta cgcgattctg cgcgagagtt acggcagttt cacggcctac
      481 atgtacggct ggatgcgttt cactatcggc gctccgggca gtgcggcttc gtacgcagtg
      541 ggagctgccg cgtttctgca ggttgttgct ccctaccacg ggcttggtat caagacctgg
      601 catgtcgctg cggcctttct cacgttcttt accctgatta actgcctgac gctatttgtc
      661 agcgccacgg tacaggttac cctgactgtc atcaagattg ccagccttgt gacaatcggc
      721 tttaccctgc tctggtatgc accggccagt acagcggtag ctcccccgac gcaatggccg
      781 ggcatgttgt ctttttctgc cgcactgatg gccgcactct gggcctacga cggctggaac
      841 aacctgccga tgctcggcgg cgaagtgagc catgcgcggc gaaacctgcc gctcgcactg
      901 gcgctcggta tgggtatcgt tctcgcgacg tacatgctcg tcaacgcggg ttatttccgc
      961 gttttatctt tttctgcagt cgtggcctcg aacccctcgg tccgcgaagg agcacagcct
     1021 gtagccgcgc tcgcgatgca caccgtaatc tctgatgcga gcgtgcgcgt ggtagctgcg
     1081 ctcatggtga tttcagcttt gggatctctg aacggttcgc tgctctcgag cgcgcgcgtg
     1141 ccctatgcca tggcgactga cggtgttttt ttcccgccgc tggcgcggct cagtcgcaac
     1201 cacgcgatac ctgtactctc tacactcagc cagggtgcga tcgcgatact gctggcaatg
     1261 tcgggcacct ttgaccagct caccgattca gtcgtattcg cgtcgtggat cttctacgca
     1321 ctgacggcgg gtgcagtctt tcgcctgcgg cgtcgtgcgg acaaatcaga gtcgatcttc
     1381 catgtgccac tgtacccggt tttgcccgcg ctctttattg cctgctcggc ggcgttcatc
     1441 gcgtacgcca tcttcgccat gccctacctg actggtatcg gtctgttggt gatagctttg
     1501 ggagcaccca tctatatgtt attcaggcgc cggcgctgag gacggcgcta tttttcgatt
     1561 gagattatac ggtaacccgt acgcttcatt gggctgccgt ccatttcggc cgggtattgc
     1621 cccatcgagt cttttgcggg tgttgtatct tcaagcacag cgcgcagctt tacagttttg
     1681 ccatgcagac tgcgcagctt ctgcacgccg acgctctctg aactgtagag cgcgaagcgc
     1741 ccctcgcgcg tggtgattgt caggtcattg ccaaggtatg catcgacgct tttgggctgc
     1801 tgactgattc gtcgtagttg tgtatctcga tcgactgaaa acccgcaggg ccaaagttcg
     1861 cgattacaag caggataatc tccatggcta tttctctttg gcttcgcgga ttgccttttc
     1921 aatatctttc gacatctgcg caagtacctg cgccctgaac tggtcgatgt ttgccggccg
     1981 caagccgcct gaatcgccgc tgaaggtccg taccgggtat tcattctgta ctgtcagaac
     2041 tgtctgtgag tcaatattgg agacgagccg tacgacgatc tgaaactgga aggtatcgcg
     2101 tttgtctttt ttgaaccatt tggcgccgcg gcgaaaatag gtgccatcac ccttgtcgtt
     2161 ttttgcatca tacttgcctg gcccttcatc gggcaattca gcttcgacga tttcggagtg
     2221 cacctcgtgc gcggtaattt caccgagcat cgtgccttcc gcaccgagca gctgccccgc
     2281 ttccagggct ttgttctcaa tgaggccggt ctgcgcgagc tctttctctt tgagaatttt
     2341 gtcgatcttt tcgcgctcta gcaccttgaa gcccgaggca accatcgcgc tgcggaaaat
     2401 ctcggtgctt tcgcgggtta ccttggcgtc agcgactgtg gaagaaaacg gcatcaccgc
     2461 gatagtctgc atttgccgca gagcatcttt tcgtatggag atcttggaag tcgaacaggc
     2521 tgctgcgagc agcagtgccg gcagtagccc cagaattgca tattttctca tggatacccg
     2581 tacgccgact ggctgcacgg tcaacggtat ttgcgtatac cgtgcccggt gttgcaatcc
     2641 gtgatatgca acgcccggca ttctttttaa aatttgccgc ccatgtgcct gattgatata
     2701 gggttagacc taaggcgtcg gccgctatgc aagaaggctc accagaatat tcacaatcag
     2761 caattgttgt tcaggatcgc tcatcgctga ccgccccgaa cagcgccgac atggaagtcc
     2821 tggcgaaact cgatgaaaag ctgcgcttca tcgaggctaa aaaagtcagt gttaatgacc
     2881 ctacctacta cctggcgctc gaccatattt ccgtggcaaa cccgacggcg ctcgaagcgg
     2941 cggttaagaa agtcacggac attgcgaatg gtggcaagaa ccgggttgca tacctttacc
     3001 cgatgctggt atcgcaccat tcaaaaccca tcttccagtt attccttttt tatccaccga
     3061 agaacaaagt aggtgtgcgt gatgcaggtc cattgcgccg cgcagcgctg gccaaatctg
     3121 aaccgctgat gcgctccctg cttgaggcca aacgctccaa gccgactgac atcgaagaag
     3181 aagatcctgg tgccgtgctc gtcaaacccg atgcaagggg aggcaaattt taccccgact
     3241 ttttttccct cgagtacgat tttcgccgcc tgctgaaaaa aagctacgac aagctgcaga
     3301 tgccttatat cgagattccc gatttttttg aagatctgcg cggtttcttc gagcgcaaca
     3361 gcgacctggt cgatcgtatc ggagaccgtt accatattgt tatcgaccag ctcatgctcg
     3421 atgacggtgg caactacgca cgggttcccg aggtgatacg ccattatcgg gtgctcgcag
     3481 acgggcttga aaagtttgta ctcaatgttt tgcatgatag ggcaaaaaaa ggcggagcca
     3541 gtgcgtacgt agcgcaactc gataactacc gtgcgacgca tgtggtgacc gcacagcccg
     3601 gcaggaaaca gagcaaagaa aaagttgatg tactgatgaa actcgcggcg gagtacccct
     3661 ttgaccgcat acaggatcgc gagaacaatg cgctgcgtga gaagctgaac gagactctca
     3721 gctggctggg cgtactgatc gaaaaaaagg accgcctcgt gaatcgtaag cagcagcaga
     3781 cggtagacaa cctgctgcaa aaaatcgaga accttataca atccaacacg cggcagaatc
     3841 tcgatctgct gcagatcgac ctctaccgcg agatcacgcg gacagtgaaa gacgacgcgc
     3901 aggcgcaggc gatggccgca gacttcgaga agcgcctcat gacgcggttt ggttcttaca
     3961 aggtcacagc gcaggatggc acttccctct ggtattttct cgaccagaag tacatgagct
     4021 ccgtgctcga aaacctgagc cgcctgatgg tcaacgatgg gggagctgca gagcgctacg
     4081 agattgccac gcagatctat gagcagctag ccaagcgaaa agaccctgct cttgatcagg
     4141 gtttgagcgc agaagagcgc atcaacttgt cgcaaatgcg ggcgcacaac gtcaacaaga
     4201 cggccgagaa gaagcagctg gagcacaagg caaaccggta taacattcca cagggggtgc
     4261 ttgtttcgct tatttctgct atactctttg cggctgccat gttttactca acgcagcttt
     4321 tctggctggt atttccgctg accctggttt cggtaatcgc tttttaccgt ataaactttc
     4381 agtcttcacc caagacccgt tcgggcgaaa aagaaagcaa aagccttgaa gctggcgcag
     4441 atgacagctc gagccagatg gcgaagcaga tgcaggaaca ggccaacgct acccggcaga
     4501 aggttgctac ctatggcgcg gcgatggttt ttccctatat gcctaagaaa ttctctgaac
     4561 gcgtattgac cgaagacgag tttcgcgaca aggtacgcct ggccgcaccc gaggtgaaac
     4621 accaggtgcc ggaactcggc ggcttagatg gcaaggcgct ggagcaggcg gtctacgaag
     4681 cattgcgcga agaggctgtc tgcattgtga ttccgaccga tatcgtgaaa aaatctctca
     4741 acaaggtgcc acccgcgctt tacatcgact acaaggactt tgcctcggac cagacgcgga
     4801 acaagatcgc cgaagaatac cgtgcaatct ttaaacccaa cctcaaggac tttgagcagg
     4861 atttctaccg ttacctgatc aacacgctcg aagtgaaata ctacacttac ctgaagaaga
     4921 atctcaagaa gtagtttttc ctgccgcaga ctagggatgc agcgcggcga gcaactcgtc
     4981 gccccagttt gcttcggcgc ccagcgcggt caagttgcca atgaggcccg ccactgcgcg
     5041 gtcgacaaac aggacatcgc cgggaaaagt gatctgtttg agctcatcca tgttctcgcg
     5101 gttatagttg atgattgcct taatcgggtc ggtttcggtg cgaaacttgt agggacgcac
     5161 ggcgaacatg ggcgaaaggg tgctgcgaaa aagttcaacg tgtttgtaag gtaccggttc
     5221 gccgttcttc aggtgcacct gcatcgaggc ggcaattgag gttagctcgt cgaagcgccc
     5281 cgcctggcaa acgtcgatag ttctcatgta gccctggtgc agccaatcgg gtaattcttt
     5341 gatgctgcca aaatcataga gcgcgatgcg gccgctgtcg agaaacgcat agttcgctgg
     5401 attcggatcg gattgtagtt tgcgcagcgc cagtagttga cccgtgacag agcggaagag
     5461 cagtctgccc caggtgttct tctcgtgctg gctgcggtgg cgcgcctctt cgatggtcag
     5521 gcccttgagc atctttgtcg ttagcacgcg gcgcgatgac agttcttcga tcggttcagg
     5581 tatacagaac tcgggatggt gtgcgtagaa agtgcggaac tcagagattc gcctgagctc
     5641 cctctcgtaa tcgatttctt cgctgaggcg cgcttcgatt tcagcaaaca ggtggtcgag
     5701 gtttgccgcg agcatactct cgagcaccag ttcaaacagc cggcgcaggt ttttcagatc
     5761 ggagaagatt gcctcgtcga tacccgggta ctgtattttg acggccactt cggtaccgtc
     5821 gtgcagcacc gcacggtaga cctggccgat cgaggccgag gccatcggct tctgctctat
     5881 ttctttcacg agggtgcgcc aatgcggaat ctcgctgtcg agctggcgga acatgatgcc
     5941 cgaggcgact accggggcat cgcgctgcag ggtcttgagc acaaaggcga gttctttggg
     6001 tagcaacgct tcctgcaccg aaagcatctg cccaattttc attgcgccgc ctttgagctt
     6061 gccgagggtt tcggcgattt tctcggcatt ttttgcccag gctttcgcca tacgcatttc
     6121 acgcgagtct tcagaaagaa acaggccaag cgctttctcg ccgacgagcg agaaccccac
     6181 cctgcccgcg agcgctccca tcttgagaaa gcggccaaag ggcgacgtgg tgattttttc
     6241 ctgctcggac ataacctctc agtagaagat tacttcatgc ccctgcagct ggccattttc
     6301 gtaacggaat ttggcagaga agaacttcgg ctgcttgagc cagccgagaa aaatgcggtc
     6361 acctggccaa agcggcagct cgagcacctg ctcgtcgggt acccagacaa ggtcaccttc
     6421 ggggcaatcg ggtttgattt cgcccgtaaa gttctgcgcg gtgtagagaa aacagtacca
     6481 gtcgtgcccc ttggcgaatt gcggaaaggt catgacgccg cgcaattgcg gtacgatgcg
     6541 caggttactc tcttcaaaca cctcgcgcac cacgcactct tcgggcgatt cgcccggctc
     6601 aaatttgcca ccgagcccat tgtatttgcc gaggtgcaca tcgtcgggcc gcttgttcct
     6661 gtgcatcatc agggtctttc cctcgtgctg cacgtagcac agtgttgcaa gaatcatggc
     6721 gtttttccgg gttcgtaagc ggttgcagcc atgcggtcgg ccaggccttc aagcatacgg
     6781 tcttcggcgg taaaccgggt ttcgaacatg ggctctccca ctgagtacgc agtcatgtag
     6841 cgaatggtgt gttgctcgta gggagcttct tcggtttcgg tagttgcccg cgcccgcagg
     6901 cgaaacgagg cgacgatcgc gaggttgtac gtcagcgggc ctgcagcgcg gtcgtacaca
     6961 ccgggctctt cggagtactg caggattttt ccgtacatga tgagctccgc cttgttctta
     7021 tcaggagtga tagtgagcga agcgctgcgc gaaaatgcta tctgcagttt ttctttcagg
     7081 cgccctgtca gctgcggcga ataggtgcgg ttatcgaaat tctggatata gagcagccgg
     7141 gtcttaccgc gaaactggcg gtagctctcg ctcagtgctt cggactcgat ctctgcattg
     7201 ccgcggccaa gactggcgca gtgtacaagc gacgcgagcg tgatgcatgg taagagaaaa
     7261 cgcagtctca ttggatagtg tttgtgaaac cagccaggct gccgaaccgc tcctggtttt
     7321 cctgcagttc agtctggtac aaggcgatcg cggcaatcgc cgcagccagc gcggggttgt
     7381 tctgcggcgg agcgaattct ttctgcaggt aacgcgtgta gtgccgcacc tcgcggaaca
     7441 ccgggtcgcg cagcagttgc cgcagcagtg gaatgttggt gtggatgccg ttaatcacgt
     7501 agtgccccag cgccgtgtgc agctggtcca tcgccgcctc gcggtcgggc gcgtgcacga
     7561 tcaccttcgc gatcatgttg tcgtacttgc ccgaaatttt ctggcctgca gcaacacccg
     7621 aatcgatgcg cacgagtgct ggttcgctgt actgcaaaat ctcgcccgtc gagggttgcc
     7681 aggttccgtc gctcttttcg gcgcagacgc gtgcctgcag agaatggccc gtgcaggtta
     7741 ctgcgctata atcggttata gcaccctgcg cgataagaat ttgctcgcgc acgaggtcaa
     7801 gacccgtaat catttccgtt acagtgtgct cgacctgcaa acgcgtattc atctcgagaa
     7861 aataaaattc ctgtcgatgg ctgtcgtaga ggtattccat cgtacctgcg ttgctgtaac
     7921 ccacgtggtt ggcgagcctg attgctgaag cgtgcagttc ggcgcggatc ttatcgggca
     7981 caccgggcgc gggggcttct tcaataatct tctggttatt gcgctgtgcg ctgcagtcac
     8041 ggtcgaacag gctgaagttt ttgcccgctg cattacccag tacctgtact tcgatatggc
     8101 gtgccttggc caggtagcgc tctgcgacga gcgagcgact gcggtagagc cgttccgctt
     8161 cgcgcagaac cttgtcgaag gcagcgtcga tctcggaaat atcttcgata attgcctgtg
     8221 ctttgccgcc accaccggcc gatgccttga gcacgacggg caccccgatc ttctctacca
     8281 gctggcgacg cgctgtggaa tcgagtaccg cgccgtcttc cagttcctca ccgggcagca
     8341 ggggtacgct cgcagcacgc gcctcagcac gcgcactggt cttttcaccc atgcggtcca
     8401 ttgcatcagc agagggacct atgaacgtga tgccggcctt ttcgacccgg cggacaaaag
     8461 ccgaattttc tgacaggaaa ccatagcccg ggtgaatagc gtcgaccttt tctgcggcac
     8521 agatctccag gatacgatcc tggtcgaggt aaccgtcgtg cagtatgtat gcagcatcag
     8581 cctcgcgcac gtatagggca tcgtggtctt cctgttcgta gatggctacg cagctaatgc
     8641 ccatagtgcg ggcagtgcgc atgatgcggc gggcaatttc gccccggttg gcgataagaa
     8701 tcttttttat cggtttcata gctattggtc gactgtctgg ttcaaaaggc gtttcgtctg
     8761 ttccagtcgt tttctcatcc tgaccagcga aactttggag taaattgcat cttgaggaaa
     8821 gtttagcatc gtttcatact gccgcgccgc agtctggtaa tcctgcgaat agaaagccga
     8881 ctcgccgtaa tagaaatgca gttctttttt gtacgctacc cttgtattga cgcttctaaa
     8941 ccacaaggtg gcatcgtagt gttttttcat cgtgtacgca accttgccca tgccgatcag
     9001 tgcatcatta aaacctgaat cctgcttgag gcagtgctta aaaacattca gcgcttcaac
     9061 cgtgcgcttc tgctcgagca gtgagtcgcc cagcgctaca agagattcaa ggttatagat
     9121 gtcgtacctg taggcacgga ggtagttgta gtccgcggca tcgcgctgcg ctgcgagttc
     9181 ataacttttt cccaggtagt agtagttctt gctgtattgc ggtacaagcg cgatacactt
     9241 ctcaaattca gcgatagcct cgcggtgctg tttgaaatag agataaaccc tgccgaggtt
     9301 gaagcggccg ggaaagaact gcggttcgtt ttgccgcaac tcttcaaaga tctcgcgtgc
     9361 gcggtccatg tcgttggcga gtacgaacgc aagaccttca ttgttctttc ctgttgccag
     9421 gtcgttgggg ccttcagacc agagaaactc cccccggttg gtgatacgtg gtctcttgtc
     9481 actggcaagc ggctcgggtt tgcccgggtc tgtctttctc aggtcggctg cggtgatacg
     9541 atcgggatac ttacgcgcgc tttcatcgac gataacccag cccatctcga agtcgagtgg
     9601 cgtcggctta aagctcgaaa ggaactcggg agtaattttc gggtggtgta gcttgaggtc
     9661 cgggtgaaca ggatctttgc gccgggcaga gctgccgtgc cagacctcgc taacggaaaa
     9721 aatttcggcc gcgagcagca acgctgccgc agcggtcagg attctcagag caggtgtgat
     9781 tcgcccctct tgtctttgta aagttggtcg cgccattcga gcaggtcggc gaattcattc
     9841 gccaggtctt tttgagtgaa aacctcgcgc tgcgcgggtt ttagcctgat gtagtgatcg
     9901 cccacgggac gtacgaactg cagcgtcgct gctatggtta tatcggcgta ggtgaagtta
     9961 tcaaagatga atcttttatt acccagtgcc ttgcgcgcct ggttaaggac ttcgcggacc
    10021 ttcttcttct cttcggcaat gatcgcgtga cggaagtcgt atttcatacc gagataagcg
    10081 acacccaggt cggccagggg cgtcaggacg ttctttgtga actcgggaaa aaagtcgggc
    10141 aggctgtcgc gttttgcgtc ttcgttgtgc gctgtggcgt cggttgcaat aatccgattc
    10201 gacttgcgtg cgatgtcaca gcggtcatgc caggctctca cttcgtcgac catgtcgagt
    10261 ggtatgagct tttcgcggtg cccttcacgg tcagcgtacg caacaatatc ccacgagtcg
    10321 gtgaaggcgc ggccgttggc taccaggata ggtgcgctga ccgggccggt gagtttacct
    10381 gcagcaagcc gcagaatcgg cgttgtgatc atgggcacgt gctcaaaata gctgtaacgg
    10441 attacatgat ggtcgagcgc ccagcgtgcc tgctcggtcc acattgaata cgacaggcca
    10501 aagaaacgat tgctcatgag gcgaacatgc ctgtcgcgcc tgcccgtaaa gcgatatgcc
    10561 gcagcaaatg cagcataccg cctaagcggg aaagcgccgg caattatata taaatcgcgg
    10621 cgatcagcgc cagaatcggc acaaggatca gcgttgtgta agccatgtaa ccgaagaaag
    10681 aaggcatttt gatcttcgat tcttcgcaca ttgctttcac catgaagttg ggagcgttac
    10741 cgatataggt gttcgcacca aagaaaaccg cacccaccga aatgccgacg agtatcgatt
    10801 cgctgatagc gccagtagtc gccttcacaa catcacccgc agctgcaccc atggacgagg
    10861 tcagaccctg cccaagggcg aaaaaggtcg cgtacgacgg agcgttgtcg aggaacgagc
    10921 tcaagccccc cgtgatccag taaaattgcc atggctggct gacgcccgtt tcggcgccgc
    10981 gggccttcag tatgcacagc gctggaatca tggtaatgaa aataccgatg aagagtgccg
    11041 cgacttcgat gattggtgca aagccaaagt ggttgcggtc gcgcggggta atcgggtgat
    11101 aatgcgagag cgaacgcaag actttatcgt caacctttcc ttgtgttttt atctgttgga
    11161 taaggtcttc ttcatgcttc ttgcgcttgc gcgtgcggtc catgtagtcg agcgagaagg
    11221 aaagcatcgc gagcgccatc atcacaggct gctgcagcca acccatagcg agcgtgccgt
    11281 ggtctgtctt gtagtccttc aaaaagacaa cagccagcag cacaccgaga atgagcagga
    11341 agttgtgcgc gccctgcaca cgtatgcgcg tctggtgtga atcgtcgctg cgcaggtcag
    11401 cgcgtttttc tgctttatag gcaatgttat cccagatgaa aaagataatc agcagggccg
    11461 cgacagcagt cagccagatc ggccagagtt tgatcagagt ccagaagaaa ggcacaccgt
    11521 agaggtaacc catgaagagc gggggatctc ccactggcag cagtacaccg cccacgttgg
    11581 aaaccagcag aataaagaac acgaagatat gcgttgtctt gtggcgttct ttgttggtgc
    11641 gaatcagcgg gcgaatcagc agtaccgatg cgcccgtagt accgatgaag ctcgccagca
    11701 ctgaaccaat gccgagtatg atggtgttgg tcagcggggt ggctttgagg tcaccctcga
    11761 tgaaaatgcc gcctgaaatc gtgaaaagcg ctatcagcag cgtgataaac gcataatact
    11821 ctttcagggt ttcgacgatg acctcgatac cgaggtaacc cagcgaaacc aggtaggctc
    11881 ccatgatgag tgcgagtagc gcgctgataa ccgctttcac caggttggat tcccagaggt
    11941 gcggtatcaa gagcggcagt accgcgatcg ccagcaggat aatgacaaat ggaataaccg
    12001 tccagatcga cagctgtttg ccgagctctt tctgtgggtc taccgtggca tggccgctat
    12061 cgtggctggc cgttgcctgt gtttcggttg ccgccagctg gcccgtatcg gccaacagtg
    12121 gcgcctgtgc catggcaccg gtcagcaaca tgcacgcgag aatttgcgag atgcgtttca
    12181 ttttttacct gtcctgatag aaatttttta tggaatgata ctgaaggtta cccgccgcga
    12241 ggcgacgcgg cccgccaccg tacggttgtc gccgactggt tcgctgtctg ctgcaccgcg
    12301 cgtttcaatt ctgtcggccg ccaccccgcg gctaacaagc gcgtctttca caacttgtgc
    12361 acgcttttca gaaatttcct ggttgatcga ttttggtccc agcgtgcagc tgtggcccgt
    12421 gacacggatg cgcgcattct cttttttcag ccgctcggcc agtgcgtcga gcacggttac
    12481 cgaggctggt gttaactcgg ttttttctgc gcgaaaggca agcttctgtg cagcgacaaa
    12541 ctcgcgtaat tcggccgaag cggcgggtgc tgcaggttcg gccgcggccg gggcgggcgc
    12601 cggtgttgcc tgcggagtcg cagcaggggg agcttcaaca gtctgcgcgg tagtttccgg
    12661 cttcggggta gcacagccgc tgcggtagac gatgaaatag aacagcgctg ccagcgcgat
    12721 aagcagaagc agtgcgagca gaatctcttt aaaggcagag gcttttttcg ccggttgtac
    12781 agcaggcgca cttgttacag ctgagggctc tgcgtgctgc gcgacagggg cgggggtgac
    12841 ttggagaggc gcagctttcg gggctttgct tgctacggat tgtttctttt tcggcttgct
    12901 tgcggcccac ttgcgaattt cggtacggaa gaactcgtcg ccttctttgg tgaatttgta
    12961 gttcttgcgg atatactcca tcgtcagctt ttcggtgtcg gagtaattat tggcatcttt
    13021 cacgacgcgc agcagatttt tggcgtcgtt gatcgagata cgcccgtcgc cgcgaccctg
    13081 tgtcaggccg tcggcaagtt caaccatgcg gcggtcgtac ttctttccgc ggatgacgac
    13141 atagtatgaa tcagacggcg tggactcttt tgcgcgggcc atgagtgacg gcgttttttt
    13201 cacggcctct gtcaatagtt ttcgtcgcac ttcaccgggg tctgcggtgc tcgcggtaga
    13261 ggcgtaggac gaatttacct gccggtgtgc ggcggtagtg ttcgacggtt tcgtgcgact
    13321 ggcgtgttgc aggcagcccg cgcatgtcgg gcagttttac cccgtattcg tcgggtagca
    13381 gtaccggggc ggccaacgct gcgaaggtga tgtccgcggc agaaatcgcg tcaccgcaga
    13441 gaaatggcct gccatcgccg agcaggcctg cgacaaaatc gaactccccc tcgaaaatct
    13501 ggcgcgagcg ttcggcttcg tcatggttta tctgcatatc gctcgtcatt gctgcctgca
    13561 gcatgggagc aatggcgggt aagagcgccg cctgccacgg ggggattacg ggctgcgaaa
    13621 aaagctgcgg cagcgacagg cgtgcatcga acagcttgcg gtaaatgaag cgccgcgtat
    13681 gaggtcccac cgctgtatcg aaacgttctt cgagctccag ggcattaggg gcagggtaca
    13741 accagcgctg gtcatattta tcggcgaggt gccgc
//
LOCUS       DAOQJF010000040        13482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2229_length_13482_cov_5.435391, whole genome shotgun sequence.
ACCESSION   DAOQJF010000040 DAOQJF010000000
VERSION     DAOQJF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2229_length_13482_cov_5.435391"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /locus_tag="PLF85_03615"
     CDS             <1..1742
                     /locus_tag="PLF85_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNE18775.1"
                     /translation="AAAVSPKPRRQSVRFFEPSVETEPGAQKGASAADFDDEPAALAD
                     EPEQQAVVVQPQKIERAEKAVIAPAKRTVEEDSISRGNELDSAQRDSLGDRASVTPRV
                     VVNRQQELRFPLLKTQRKALEFANPAEAQSVIERLEETLEQFSIEGHVVGIQRGPIIT
                     RYELKMAAGIKVSRILALTDELAMALEAMRVRIEAPIPGRSTVGIEIPNKQRIKVLLG
                     DILNDVGYREFSGALPLPLGRDIAGNLLLEDLTRMPHLLIAGATGSGKSVAVNSFISS
                     LILHKTPEEVRFLLVDPKLVELSGYNDIPHLLHPVITEPNKAILALNWAVEEMERRYE
                     DLAEMSARDIRSYNEKIRSQKNKAESPLMPYIVILIDELGDLMMVAGKEVESSIIRLA
                     QKARAVGIHLILATQRPSVDVITALIKANCPARIALQVAQKTDSRTILDGNGAEQLLG
                     QGDMLFRHPSKGQLIRAQVPLIEDNEVEDVVAYAKKLARPAYITLEDPKGKMGELEAG
                     DEELFEEAWQIIVESQKASASYLQRRLRIGYNKAARLIEAFEARGMIGPQIGSKPREI
                     IGGTGQGVQARVT"
     gene            1864..3792
                     /locus_tag="PLF85_03620"
     CDS             1864..3792
                     /locus_tag="PLF85_03620"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE18776.1"
                     /translation="MMNRLRGLFRRNPERLQYRGEFVADMDRQLSSWTRIGAAIATFA
                     WLDFAFNTDKKLHPEFPELIYFRLGLTAIGALIFLTSFIPFFRRHGALLLKIMIGYVM
                     CATSFFTGRIADDPNYVSGLQIVVMIPIAAPVAFRLFLSMQLSSIAIFLAAVLVYKPN
                     LTTPAAAYSMNNLVISYVIAIAFSFLLDRIRFDSFMKTKKIEIKNTEIEAQIKRINEL
                     KTQQDGDYFLTSQLLHPLALNEAVPSRVRVDFLTEQKKKFQFRQWHSEIGGDISSANT
                     VYLRNKRYVAFMNGDAMGKSIQGAGGALVLGTVFKSFLHRTQNDEMSRFMFPEQWLRD
                     CYEELHHVMISFDGRMLVSAVIGLIDEDSGFLYYINAGHPWIAILRNGVANFIEEELT
                     LRKLGMPENDELFRVKTFRLQKGDVIFAGSDGRDDIALGRDEMGNSMINQDETMFLRI
                     IEKTGGDLHQIREELLKAGDITDDLSLVRIVFDGTGHDPISRADLSVIKKYAQTKDYE
                     GAIAELDRLYADNPHDPKIVYELALLNSRLKKFSRAAALGEEYCNYDPSDLGMIYLTS
                     RALKYCAGPEKTLLKRAADYGERLILREPMSFHGIVNLSDIYRRLEKRDKAEKLLRKA
                     EAFDPENRAVKKLQELMA"
     gene            3830..4894
                     /locus_tag="PLF85_03625"
     CDS             3830..4894
                     /locus_tag="PLF85_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNE18777.1"
                     /translation="MDKRKLLYFLPVLVLVLLWAFWPDSEESRKLRLLEDQARKAALA
                     ANPQSGEYREFLEQNRRLFSEENMYTFSQLMEMARTGRISLVSELWRLRTKCGVGAGE
                     SADGAGTATPQMNFDECNIRIENFLREQYPAPENEKIIALFRGYLRYEDAMRRFNIPE
                     NLTATERYEYIKKKRREFFSETDAQLIFGFEEARIASQDALNDFVKNSAEMPAEQRVK
                     KYYELRKNNLGEYNAAVSEAEPAYTRYETEVMLRGDEMQRKGNSAAETQALRERYFGA
                     AAAQRMAQVEREIRDERARIDSYEAAAQKFGRDNPSLSETERREKLNELRVQMLGKEE
                     AEAYERRMQYEEYLRSNNLK"
     gene            4897..6588
                     /locus_tag="PLF85_03630"
     CDS             4897..6588
                     /locus_tag="PLF85_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNE18778.1"
                     /translation="MRNLQSFYLRQALLALVHAVGLLFALSPFDLWPLGILAIAAVLA
                     AAADLHGAGYRRILASWLFFAFAATFITFGWITATIHRYTGERVLVSFVLALVYALLF
                     QLKFLGIFTLARYWNFSAVHDAGRLLAAAAVFAVAELIAPELFPWSWGNAVAAFSWLR
                     QLASIGSVYFVSLLTVFLALLGWQLLWVCRGLRFKHRLRLQLPALVLAVSGLLVGCFM
                     YYWPLQGDTGKLRTLVVQTNIGAAPEQKRSDHEFATDAINRLFNQSLEGVILYAPLDL
                     VVWPEASMPFHSADATTANRTIYSPTFDATAEYTSRRSGAALVFHDMYQSGARLYSRL
                     ATRPETGMKNYYLKRRLVPWGEFLPFGDIWPGLRHVFAEAGHYSAAQELNEITLNVGL
                     APDTLRSRQQIEPELAFLSSGATVRKHLPQPQLMRQYIVKPAICYEALYPADVRTRDA
                     QVILNLASDAWFGDGVEGHQHASAAVLRAVENGVPIVRAAMSGVTVIADAKGDDIVPR
                     SLQGRPQNVFAEIPLARRTTVFSRFGLSAFWALALACLWPWLLQVIISYRTAKSK"
     gene            complement(6557..7240)
                     /locus_tag="PLF85_03635"
     CDS             complement(6557..7240)
                     /locus_tag="PLF85_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HNE18779.1"
                     /translation="MPDHVLASTLPAAVQTKLLKILASKAQRAGEYCLPFDPTQFPFS
                     LRTLFPEFSRYALEIGCGWGEYTRASAQAHPDTLVIAVEKKLARVIASSRTQRAEGIS
                     NIRYLVLDLAWFFDGVFAEAQFDAITINFPDPWPKARHHKHRFVSPDFGESLSRIARP
                     GARLTFATDNYPYAREAMETLEASTDWHNAVAPWIAVGDIAGRPRSFFETVHRQEGAP
                     IYFLQYDRK"
     gene            complement(7253..7900)
                     /locus_tag="PLF85_03640"
     CDS             complement(7253..7900)
                     /locus_tag="PLF85_03640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE18780.1"
                     /translation="MKITARLMQFETNSPSGNLEILFCDWEIPREQVRGEAPVQKGVC
                     FIPADLSAVAHVRSYIEHVANTFSLSEQSIFELALVADELVANAILASYAKNGNENIV
                     LKWTMKDNIFTLSVLDYGGGFKITEVFDEIPRGSDLASFLRSLKEYREARRAVIPLNG
                     ALIEHVRFGRGLRIISNLVRKLDIEFHDADGSTVHEPTANTVGSIITIEYVAQAA"
     gene            complement(7897..9450)
                     /gene="gltX"
                     /locus_tag="PLF85_03645"
     CDS             complement(7897..9450)
                     /gene="gltX"
                     /locus_tag="PLF85_03645"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNE18781.1"
                     /translation="MQVRTRFAPSPTGYLHVGGARTALFNYLYARAQGGKFILRIEDT
                     DQARSTEQSYHQVIDSLKWLGYEWDEGPEVGGPHGPYKQSERLQIYKDNAEKLIQEKK
                     AYPCFCSADELERKKKQREAMGLPYVYDGKCRNLTAEEVAEKKAQGIEHTVRFRTPAK
                     EVVVEDIVQGTVRFDTALIGDFIIVKSDGFASYNYAVVVDDHLMDITHVIRGVGHLSN
                     TPRQIMIYEAFGWKEPRWAHVSEIVGSDHKKLSKRHGATPITAFRDLGYSARAFNNYM
                     ALLGWSSPDGREYMSIDEQKSLFDVARCSKSPAMFDVFDLAKAAEVDLAQASIAELET
                     YLFAKSKLNWLSNQHIRATPDNQFIEEILPFVKATGVVPDAECTAGNERLRGILLSLR
                     VYLDTLGQIRNYIGDFYRAFEPKREDYVTSEYFQAENFTSLATVLLRHIEQMGGSSDV
                     LEADAIKEALNAVGKETGTKGKPLFMTLRVASTGRLEGLELPVYLGLLGKARVIERIT
                     KMIALKNQA"
     gene            9621..11270
                     /locus_tag="PLF85_03650"
     CDS             9621..11270
                     /locus_tag="PLF85_03650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNE18782.1"
                     /translation="MKRDLQRLQNQVFDILVLGGGVSGASIAWDAALRGFSVALIERK
                     DFGHATSAATSKLIHGGLRYLAQGDIPVVRESLRERRYLEMNMAHQVFPLPFLMPFYD
                     FTPTPRWMLGVGLTLYDLLSWDRNYIDDPDKHIDRKRWLSREKALQLEPGLNPQGLKG
                     AYHYYDALNRHPNRSNLEYVLSAASRGAQVANYVACDEFIIEETSAGKRVSGVIAKDT
                     LTGRQFPVRAQVTVNATGPWGDIVLGKLHGAAEHKLVRSKGIHLLFPRFHKNTTVALE
                     TRDKHHIFLIPWGNYSLLGTTDTEFKGDPDDLNVTRDDAQDFLDLFNSYFPQKKTLGD
                     VVNSYAGLRPLVAESSVTSTYKASRRHEIVLHRKQDNVDGLVSVFGGKWTTSRALAEQ
                     TVDLIEKKFGFPRRHSISHKTPVVGGQVGTRLATFIAEAHARWDKVYSPQLINHLIEI
                     YGALYERVLDYVIRDKSLLMPIENEYPFIRAEIHYAVDREMALTLADFLNRRCGLGNM
                     GYISDSALSSVATVMGDLLRWDSTRRREEIAAFKKSRSIIG"
     gene            11278..12210
                     /gene="murB"
                     /locus_tag="PLF85_03655"
     CDS             11278..12210
                     /gene="murB"
                     /locus_tag="PLF85_03655"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNE18783.1"
                     /translation="MADLRAVTAFLDSRGIAYRLGEPLHTYTTFRIGGPADIFVLPRN
                     ADELAQVQQQTALNDVPVFLLGGGSNLLVADAGFRGVVVHPDLGEEPEILHRSADRLV
                     VRVPASARAPLAGKKISALGYGGLEFLTTIPGHFGGSVIQNAGCYGHELRDSLVTVEA
                     VRTGQVATVSNASCEFAYRDSIFKRDNSAWIASATLELAAGDSAAIAARIEDYKNRRI
                     ASQPKNRRSAGSIFKNPAPGVSPKKAWQLIDEAGLRGVSQGGAEISAEHCNFIVNNGS
                     GSAADVLHLIHLIEQRVYDTCGVRLEKEVVLVGF"
     gene            complement(12272..>13482)
                     /locus_tag="PLF85_03660"
     CDS             complement(12272..>13482)
                     /locus_tag="PLF85_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNE18784.1"
                     /translation="IKQKTAHTLKVAEDVALMKEMGANSYRFSIAWDRLFPREGMDKP
                     DAGAVAFYDRLFAELKKQNIEPSVTLFHFSSPAWFFKEKDGKRGWERADALQHFTKFV
                     RFVVGRWGEQVNVWCTLNEPMVYIYSGYMQGIFPPLEKRPDEKSVVPVMEALLRAHAE
                     AYRIIHAANPKAMVGVTQHTREFSPYRNYALLDRIIAGKVEQAFIWDFTDAISTGVLK
                     VTNTSIEKPIENLKGTQDFLGINYYGRFYIKSNIFSPTKFEIKNHDPSEESEIKNDLG
                     WADYPIGMKNVLLTGKEKYALPLYVLESGTADKASDDKLRQRLLIGHTAEIAAAIREG
                     ADVRGYFHWSMIDNFEWAEGFEARFGLVEIDYLNGFARKKRRSFDLYREIIQRGISPA
                     KYNQAAQEYQ"
BASE COUNT         2899 a         3668 c         3845 g         3070 t
ORIGIN      
        1 aggcagctgc agtgtcaccc aaaccccggc ggcagagcgt tcgctttttc gaaccctcgg
       61 ttgagacgga gcctggcgca caaaaaggcg ccagcgcggc agacttcgat gacgaacctg
      121 ctgcattggc cgatgaacct gaacagcagg cagttgtggt gcagccgcaa aagatcgagc
      181 gcgcagaaaa ggcagtaata gcgcctgcta aacgaacagt cgaagaagat tctatttcgc
      241 gcgggaatga actcgattca gcgcagcgcg attcgctcgg cgaccgcgcc tctgtcacac
      301 cgcgcgtcgt ggtgaatcgc cagcaggagc tccgttttcc tcttctcaaa acacagcgca
      361 aggcgctcga atttgccaac cccgccgaag cgcagagcgt gatcgaacgc ctggaagaaa
      421 cgctcgaaca gttctctatt gaaggtcatg tggtcggcat acagcgcggc ccgattatca
      481 cgcgctacga actcaaaatg gcagcgggta tcaaggtgag tcgtattctc gcgctgaccg
      541 acgaactggc gatggcactc gaagcgatgc gcgtgcgcat cgaagccccg ataccgggcc
      601 ggtctacagt aggtattgaa atccccaaca agcagcgcat caaggtgttg ctcggcgaca
      661 tactgaacga cgtgggttat cgcgagtttt ccggcgctct gcccctgccg ctgggacgcg
      721 acattgccgg caacctgctg ctcgaagacc tcacgcgcat gccgcacctg ctgatcgcgg
      781 gggcaacggg ttcgggtaaa tcggttgccg taaactcgtt tatctcgagt cttattctgc
      841 acaagactcc tgaagaggta cgctttctgc tcgtcgatcc caagctcgtt gagctttcgg
      901 ggtacaacga cattccgcac ctgctgcacc cggtcattac cgaacccaac aaagcgatac
      961 tcgcgctcaa ctgggcggtt gaagagatgg agcgtcgcta cgaagatctt gctgaaatga
     1021 gcgcacgcga cattcgttcg tataacgaaa aaatccgctc acagaagaat aaggcggagt
     1081 ctcctcttat gccatacatc gtgatcctga tcgatgaatt gggagacctc atgatggtcg
     1141 ctggcaaaga ggttgaatct tcgatcattc gcctggcgca aaaagcgcgc gcggtcggta
     1201 ttcacctgat actggcgaca cagcggccgt ctgtagatgt catcacggcg ctcatcaagg
     1261 cgaactgtcc tgcacgtata gcgttgcagg tagcacagaa gacagactcg cgtactattc
     1321 tcgacggcaa cggcgcagaa cagctgctgg ggcagggtga tatgcttttc cggcacccgt
     1381 ccaagggcca gctgattcgc gcgcaggtac cgcttatcga agacaacgag gtagaagacg
     1441 tcgtcgcgta cgccaagaaa cttgccaggc ctgcttacat cacgcttgaa gacccgaaag
     1501 gtaaaatggg tgaactcgag gccggcgatg aagagttatt cgaagaagcg tggcaaatta
     1561 tcgtcgagag ccagaaggca tcagcaagtt acctgcagcg ccggctgcgt atcggctaca
     1621 acaaggccgc acgtcttatc gaagcctttg aggcccgcgg catgataggt ccgcaaatcg
     1681 gttcaaaacc ccgggagatt atcgggggca ccggccaggg tgtccaggcc cgcgttacct
     1741 aaagttactc gagtcgcttt cgggcgttcg gcattgaatc tgccacacag ggccccacta
     1801 gcccgtgccg ggaactgtta tgcgatagcg ttgacttagt gtgcactgtc gtcattaagg
     1861 tacatgatga acaggctgcg cggtttattt cgtagaaatc cagagcggct gcaataccgc
     1921 ggcgaatttg tagcggatat ggaccgccag ctaagcagct ggacgcgcat aggtgcggca
     1981 atcgccactt tcgcctggtt ggatttcgcc tttaataccg acaagaagct gcaccctgag
     2041 tttccggagc tgatctattt tcgcctgggg cttaccgcca tcggggcact gatctttctc
     2101 acttcgttca ttccgttttt ccggcgccat ggtgcactgc tgctcaagat catgattggg
     2161 tatgtgatgt gtgcaacttc attctttacc ggtcgcatag ccgatgaccc gaactatgtt
     2221 tcaggcctgc aaatcgtggt gatgatcccg attgcagccc ccgtggcctt caggctcttt
     2281 ctgagtatgc agctgagttc aatcgccatc tttcttgccg cagtccttgt ctataaacct
     2341 aacctgacga caccggctgc tgcatactca atgaataacc tggtcatttc atacgtcatt
     2401 gccatcgcct tctctttcct gctcgaccgc attcgcttcg actcgttcat gaagacgaaa
     2461 aagatcgaga tcaagaatac cgaaatcgag gcgcagatca agcgtatcaa tgagctgaaa
     2521 acgcagcagg acggcgatta tttccttacc tcgcagctgc tgcacccgct tgccctgaac
     2581 gaagcggtgc catcgcgcgt gcgcgtcgat tttcttaccg aacagaaaaa aaagttccag
     2641 ttcaggcagt ggcattctga aatcggggga gatatttctt cggcaaatac tgtctacctg
     2701 cgcaacaaac gctacgtagc gttcatgaac ggcgacgcga tgggcaagtc gatccagggt
     2761 gctggtgggg cgctggtgct cggtacggtt ttcaagtcat ttctgcacag aacgcagaac
     2821 gacgagatga gccgcttcat gtttcccgag cagtggctgc gggactgtta cgaagaactc
     2881 caccacgtga tgatttcttt cgatggccgc atgctcgtgt cagcggtcat cggcctcatt
     2941 gacgaagaca gtggtttcct gtactacatt aacgctggac acccctggat agcgattctg
     3001 cgcaatggcg tagcaaactt cattgaagag gagctcacgc tcagaaaact cggcatgcct
     3061 gaaaatgacg aacttttccg tgtgaaaacg tttaggctgc agaagggcga tgtcatcttt
     3121 gctggttcgg acggtcgcga cgatatcgcg ctgggccggg atgaaatggg taactcgatg
     3181 attaaccagg atgagaccat gttcctgcgt atcatcgaaa aaacgggtgg cgatctgcac
     3241 cagatacgcg aagagctgct caaggccggc gacattaccg atgacctttc gctcgttcgc
     3301 atcgttttcg atggtacggg gcatgatccg atttcacgcg ctgatctttc agtgatcaag
     3361 aaatatgccc agacgaagga ctacgaaggc gcgatagcgg agctcgatcg cctttatgcc
     3421 gacaacccgc acgatccgaa aatcgtctat gagcttgcgc tactcaactc gcgcctgaag
     3481 aaattctcgc gcgccgccgc actgggcgaa gaatactgca actatgatcc gtctgacctg
     3541 ggaatgatat acctgacctc gcgcgcgctc aagtactgcg ctggtcctga aaaaaccctg
     3601 ctcaagcggg cggcagacta cggtgaaagg ctgatcctgc gcgaaccgat gagttttcac
     3661 gggattgtga atctttctga catttaccgc cgcctggaaa agcgcgacaa agcagagaag
     3721 ctgctgcgga aagcagaagc atttgacccg gaaaatcgtg cggtgaaaaa gctgcaggaa
     3781 ctgatggcct gagccggttc gatacctgaa atgctgcggg aaaaatatca tggataagcg
     3841 gaaactcctc tattttctgc cggtgttggt actggtgctg ctctgggcat tttggcccga
     3901 ttcagaagaa agccgcaagc ttcgcctgct cgaagaccag gcgcgcaagg ctgcgctggc
     3961 agccaacccc caatcgggtg aataccgcga gtttctcgaa cagaaccggc gtctattcag
     4021 tgaagaaaac atgtatacct tcagccaact gatggagatg gcacgcaccg ggcgtatttc
     4081 gcttgtctca gagctttggc gcctgcggac caagtgcggg gtcggtgcag gagaatctgc
     4141 agacggagcc ggtactgcaa caccgcagat gaactttgac gaatgcaaca ttcgcattga
     4201 gaacttcctg cgcgagcagt atcccgcacc ggaaaatgag aagattattg cattgttccg
     4261 tggctacttg cgctacgagg acgcgatgcg ccgctttaat ataccggaga acctcacggc
     4321 gaccgagcgc tatgaataca taaaaaagaa gcgcagggaa ttcttttctg agaccgatgc
     4381 gcagctcatt ttcggcttcg aagaggcgcg cattgcgtcg caggacgccc tcaatgattt
     4441 tgtaaaaaac tccgccgaaa tgcctgccga gcagcgcgtg aaaaaatact acgagctccg
     4501 caagaacaac cttggggaat acaatgccgc cgtcagtgaa gccgagcctg catacacgcg
     4561 gtatgagacc gaggtgatgt tacgcggcga tgaaatgcag cgcaagggaa attcggcagc
     4621 tgaaacgcag gcgctgcgtg aacgttattt cggcgccgcc gctgcgcaac ggatggcgca
     4681 ggtcgaaagg gaaatccgtg acgagcgcgc gcgtatcgat tcgtatgaag cagccgcgca
     4741 gaaatttggc cgcgataatc ccagcctgtc ggaaacagag cgccgcgaaa aactcaatga
     4801 attgcgggtg cagatgctgg gcaaagaaga ggccgaagct tacgaacgcc gcatgcagta
     4861 cgaagagtac ctccgcagca acaaccttaa atagcggtgc ggaacctgca gtccttttac
     4921 ctgcggcagg ccctgcttgc gcttgtacac gccgtcggac tgctttttgc tctgtcgccc
     4981 tttgatctgt ggccattggg aattctggcc attgcggcag tgctcgcggc tgcagcggac
     5041 ctgcatgggg ctggttaccg gcgtatcctt gcaagctggc tattctttgc ttttgccgcc
     5101 actttcatta ccttcggctg gatcaccgcg acgatccacc gttataccgg tgagcgcgtg
     5161 cttgtctcgt ttgttcttgc gctggtttat gcgctgctct tccagcttaa attcctggga
     5221 attttcacgc tggcgcgtta ctggaatttt agcgcagtgc atgatgccgg cagattactc
     5281 gccgccgccg cagtgtttgc cgttgcggaa ctgattgcgc cggaactttt tccctggtcg
     5341 tggggtaatg cagttgccgc gttcagctgg ctgcggcagc ttgcttcgat cggttctgtg
     5401 tattttgtca gcctgctgac agtttttctg gcgctgctgg gctggcagct gctgtgggtt
     5461 tgccgcggac ttcgttttaa gcaccgattg cggttgcagt tgccggcact cgtgttggcg
     5521 gtgtctggcc ttcttgttgg gtgcttcatg tattactggc cgctacaggg tgacacgggt
     5581 aagctccgta cactggttgt gcaaacgaat atcggggctg cgcccgaaca aaagcgcagc
     5641 gaccacgagt tcgcaaccga tgccatcaac cggctgttca accagtcgct cgaaggtgtg
     5701 atcctgtatg ccccgctcga cctggtggta tggcccgagg cgagcatgcc ttttcatagc
     5761 gcagacgcga cgactgccaa tcgtacgatc tattcaccaa catttgatgc gacggctgaa
     5821 tatacgagcc gtcgcagcgg ggctgcgctg gtctttcacg acatgtacca gagcggtgca
     5881 cggttgtatt cccgactggc tacacgcccc gagacaggta tgaagaatta ctacctgaag
     5941 cgaaggctgg tgccctgggg ggagttcctg ccgttcgggg atatctggcc cggattgcgg
     6001 catgtgttcg ctgaagcagg gcattacagt gctgcgcaag agctcaacga aattacatta
     6061 aacgttggtc tggctcctga caccctgcgc agccgccaac agatcgaacc tgagctcgca
     6121 ttcctttcga gcggcgcgac tgtcaggaag catctgccgc aaccgcagtt gatgcggcag
     6181 tacatcgtga agccagccat ctgctacgag gcactttatc ctgccgacgt gcgcacccgc
     6241 gacgcgcagg taattctcaa cctcgcaagc gacgcgtggt ttggcgacgg agtagaaggg
     6301 caccagcatg cctcggctgc agtgctgcgt gcagtggaaa acggtgtgcc gatcgtccgc
     6361 gcggcaatga gcggcgtcac ggtgattgcc gacgccaaag gcgacgacat tgtcccgcgc
     6421 agccttcagg gacggccgca gaatgtattc gctgagattc ctttagcaag gcgaacaacc
     6481 gtgttctcgc gcttcggcct gtctgccttt tgggcgcttg cgctcgcctg tctctggccg
     6541 tggctactgc aggttattat ttcctatcgt actgcaaaaa gtaaatagga gctccttctt
     6601 gccggtgtac ggtctcgaag aagctgcgcg gccgcccggc gatatcaccg accgcgatcc
     6661 atggagcgac tgcgttatgc cagtctgtgc tggcctcaag agtctccatc gcttcgcggg
     6721 catacgggta attgtcggtg gcaaaggtta gtcgtgcccc gggacgggca attcgactca
     6781 gggattcgcc aaaatcgggt gatacgaagc ggtgcttgtg gtggcgggct ttgggccacg
     6841 ggtcaggaaa gttgatcgtg atagcatcga actgggcttc tgcaaacact ccgtcaaaaa
     6901 accaggcaag gtcaagtacc aggtaccgga tattgcttat gccctcggcg cgctgggtac
     6961 gagacgaggc aatgacacgt gcaagtttct tttcgacggc aatgacgagg gtatcgggat
     7021 gtgcctgcgc gctggcacgt gtgtactcac cccagccgca gccaatctcg agagcgtagc
     7081 ggctgaattc cgggaaaagc gtgcgcagag aaaacggaaa ttgtgttggg tcaaacggca
     7141 ggcagtattc accggcgcgc tgtgccttac tcgccaggat cttcagcagt ttggtctgga
     7201 ctgcagccgg cagtgtgctg gctagaacgt gatcgggcat gccggttggg tgtcaggcgg
     7261 cctgcgcgac gtattcgatc gtgatgatcg aaccgactgt attggcggta ggttcgtgca
     7321 cagttgaacc gtcggcatcg tgaaactcaa tgtcgagctt gcgcacgagg ttagagatga
     7381 tgcgcaggcc acgaccgaag cgcacgtgtt cgatgagcgc tccgttgagc ggaataactg
     7441 ccctgcgtgc ttcgcggtat tctttcaggc tgcggagaaa acttgccagg tcagacccgc
     7501 gcggaatttc atcgaatact tcggtgattt tgaagccccc gccgtagtcg agcacactca
     7561 gcgtgaagat attgtctttc atggtccact tgaggacaat gttctcgttg ccgttcttcg
     7621 catagctcgc gagaattgca ttcgcaacga gttcgtcggc aaccagcgcc agctcaaaaa
     7681 tgctctgctc ggagagagag aaggtgtttg ccacatgttc gatgtagctg cggacgtgcg
     7741 ccaccgccga gaggtctgcc ggaataaagc agactccctt ctgtacaggg gcttcgccgc
     7801 gaacctgttc acgcgggatt tcccagtcgc agaacaggat ttcgaggttg ccagacggtg
     7861 agttggtctc gaattgcatt agcctcgctg ttatcttcat gcctgattct tgagggcaat
     7921 catttttgta attcgctcga tgacccgggc tttgcccaaa agccccaggt aaacaggtag
     7981 ttcaaggcct tccagcctgc ccgtagaagc aacgcgcagc gtcatgaaga gcggcttacc
     8041 tttagtgccg gtttctttac ctacggcgtt cagggcctct ttaatggcgt ctgcctcgag
     8101 tacgtctgag ctcccaccca tctgttcaat atgacgtagc aaaaccgtag ccagcgacgt
     8161 aaaattttcc gcctggaagt attctgatgt gacataatct tcacgtttcg gctcaaaggc
     8221 gcggtagaag tcgcctatgt agttgcggat ctgccccagt gtgtcgaggt aaacgcgcag
     8281 cgatagcaga atgccccgca gccgctcatt gccggctgta cattctgcgt ccggcacaac
     8341 accagtcgct ttgacgaatg gcagaatttc ttctatgaac tggttatctg gggtcgcgcg
     8401 gatatgctgg ttggaaagcc agttgagttt tgatttggca aaaagatagg tttcgagctc
     8461 ggcgatcgat gcctgcgcca ggtcgacttc tgcggctttt gccagatcaa aaacgtcaaa
     8521 catcgcgggc gatttgctgc agcgcgctac gtcaaacagc gacttctgct cgtcgatgct
     8581 catgtattcg cgcccgtcgg gtgatgacca gcccagcagt gccatgtaat tgttaaatgc
     8641 acgcgcgctg tagcccaggt cgcggaaagc ggtgatcggc gttgcgccgt ggcgtttcga
     8701 gagtttcttg tggtcgctgc caacaatctc gcttacgtgt gcccagcgcg gttctttcca
     8761 gccgaacgcc tcgtagatca tgatctggcg cggcgtattc gagagatgtc caacgccgcg
     8821 gatcacgtgc gtgatatcca tgaggtggtc gtctaccaca acggcatagt tgtacgacgc
     8881 aaagccgtct gatttgacga tgataaaatc cccgatcagc gcagtgtcaa agcggaccgt
     8941 accctgcaca atgtcttcca ccacaacttc cttggcagga gtgcggaagc gcacggtgtg
     9001 ttcaatgccc tgtgcttttt tttctgcaac ctcttcggct gtcaggttac ggcacttgcc
     9061 gtcgtagaca tagggcagtc ccatagcttc acgctgtttt ttcttgcgct cgagttcatc
     9121 ggcgctgcag aaacaggggt atgctttttt ttcctggatg agcttttccg cgttatcctt
     9181 gtagatctgc aggcgctcgg actgtttgta ggggccatga ggtccgccaa cttcagggcc
     9241 ttcgtcccat tcgtagccga gccacttgag cgagtcaatt acctggtggt aactctgttc
     9301 ggtcgagcgt gcctggtcag tgtcttcaat ccgcagaata aattttcccc cctgcgcgcg
     9361 cgcgtagagg taattgaaca gcgctgtgcg ggcgccgcca acgtgaaggt aaccagtggg
     9421 tgagggggca aaacgggtgc ggacttgcat gggatttatg atgtgacaat aatcttatcc
     9481 tgaagcgaag atagcgggtc aatgaactat gcggcgtgcc cggtacgcag tgcagcgggt
     9541 agtggtcctc tctcaggcag gtgagcgggc gcgttattcc tttacggcat gtcttctctg
     9601 cctgaggggg aagctaatcc atgaaacgcg atctgcagcg gttgcagaat caggtgtttg
     9661 acatactggt tctgggcggg ggagtcagtg gtgcctcgat cgcatgggac gccgcgctgc
     9721 gcggtttttc cgtcgcgctc atcgaacgca aagactttgg ccatgccacc tcggccgcaa
     9781 cctcaaagct cattcacggt ggcttgcgct acctcgcaca gggtgatata cccgtagtgc
     9841 gtgaatcgct gcgcgaacgg cgctacctcg aaatgaatat ggcgcaccag gtatttccgc
     9901 tgccatttct catgcccttt tacgatttta ctcccacgcc ccgctggatg ctcggcgttg
     9961 gtctcacgct ctacgatctg ctctcgtggg atcgcaacta catcgacgac ccggacaagc
    10021 acattgaccg caaacggtgg ctttcacgag agaaggctct gcagctcgag ccaggactca
    10081 acccgcaggg gctcaagggg gcataccatt actacgatgc cctgaaccgg cacccgaacc
    10141 gctcgaacct tgagtatgtt ctgtcagctg cctcgcgcgg tgcacaggta gcgaactacg
    10201 tcgcgtgcga tgaattcatc atcgaagaga cctcggctgg caaacgcgtg tcgggggtga
    10261 tcgccaaaga cacgctgacc ggcaggcaat ttcccgtccg tgcccaggtt actgtgaatg
    10321 cgaccgggcc gtggggcgat attgtcctgg gcaaacttca tggtgctgca gaacacaagc
    10381 ttgtccggtc gaaaggtata cacctgcttt ttccccgttt ccacaagaac accacggttg
    10441 cgctggaaac gcgcgacaag caccatattt tcctgatacc ctggggtaac tactcgctgc
    10501 tcggcacgac cgataccgag ttcaaaggtg atccagacga cctgaatgtc acgcgcgatg
    10561 atgcgcagga ttttctcgat ctcttcaact cttactttcc acagaaaaag acgcttggcg
    10621 acgtagtgaa ttcgtacgcg ggactgcggc cgctcgtagc cgaaagctcg gtgacaagta
    10681 cctataaagc ttcgcgccgt cacgaaatcg tgctgcaccg caagcaggat aatgtcgatg
    10741 gcctcgtgtc agtcttcggc ggtaagtgga ccacctcgcg cgcattggcc gagcagactg
    10801 tcgacctcat cgagaagaaa tttggctttc cgcgccgcca ttcgatatcg cacaaaacgc
    10861 cggttgttgg cggtcaggtt ggcacgcggc tggcgacttt tattgccgaa gcgcatgcaa
    10921 gatgggacaa agtttattca ccacaactca tcaaccacct tatcgaaatt tatggggcgc
    10981 tctatgaacg tgtgctcgat tacgtgatac gcgataaatc gttgctcatg ccgatagaaa
    11041 acgaataccc gtttatccgc gccgagattc actatgcggt cgatcgcgag atggcgctga
    11101 cgctggccga tttcctgaac aggcgctgcg gtctcggcaa tatgggttat atctctgatt
    11161 ctgcgctcag ctcggttgcc acggtaatgg gtgacctctt gcgctgggat agcacgcgcc
    11221 ggcgcgaaga aatcgctgcg ttcaagaaat cccgttccat tatcggctga acttgccatg
    11281 gctgacttgc gcgccgtcac agcgtttctt gactcgcgcg gcattgcgta tcgcctgggc
    11341 gaaccgctgc acacctatac aaccttccgt attggcggcc ccgccgatat cttcgtgctg
    11401 ccgcgcaacg ccgacgagct ggcgcaggtg cagcagcaaa cggcattaaa tgacgttccg
    11461 gtttttcttt tagggggagg ttcaaacctg ctggttgccg atgccggatt tcgcggtgtt
    11521 gtcgtgcacc cggacctcgg cgaagagccg gagattttgc accgttctgc cgatcgcctg
    11581 gttgtgcgcg taccggcgag tgcccgcgcc ccgcttgcgg gcaaaaaaat ctctgccttg
    11641 ggttacgggg ggctcgaatt tctcacgaca ataccggggc atttcggcgg ctcggtcatc
    11701 cagaatgccg gctgctacgg ccatgagctg cgcgatagcc tggtaacggt cgaagccgtc
    11761 aggacagggc aggtcgcgac agtcagcaac gccagctgcg aattcgccta ccgcgacagc
    11821 attttcaaac gcgataattc ggcctggatt gcatccgcga cactcgaact tgctgcgggc
    11881 gacagtgcgg caattgctgc gcgcattgaa gattacaaga accggcgcat tgcatcgcag
    11941 cccaaaaacc gtcgttctgc cggcagcatt ttcaagaacc ctgctccggg cgtgtcaccg
    12001 aagaaagcct ggcagctcat tgacgaagcc ggcctgcgcg gcgtaagcca gggtggggcg
    12061 gagatttcag cggagcactg caattttatc gtgaacaacg gcagcggcag cgcggccgat
    12121 gtgctgcacc tgattcacct catcgagcag cgcgtctacg atacatgcgg cgtgcgactc
    12181 gaaaaggaag tcgtgctcgt gggtttctga aaaaaaaaag tatcttagac ctgcgcgaca
    12241 tctcgcttcg gcgagatgtc gcgcaggtta tttactgata ttcctgcgcg gcctggttgt
    12301 atttcgcggg cgaaattcct ctctggataa tttcgcggta aagatcgaag ctccgccgct
    12361 tcttgcgggc aaagccgttc aggtagtcga tttcgaccag gccgaaccgc gcctcaaacc
    12421 cttcagccca ttcgaagttg tcgatcatcg accagtgaaa atagccgcgc acgtcagcgc
    12481 cttcgcgaat cgctgcggca atttctgccg tgtggccgat caacagtcgt tggcgaagtt
    12541 tgtcgtcgga cgccttgtca gcggtgcctg attcgagtac gtagagcggt agtgcatact
    12601 tttctttacc ggtcagcaac acgttcttca tgccgatcgg gtaatcagcc cagccaagat
    12661 catttttgat ttcgctctct tcactgggat catggttctt gatttcaaac ttcgttggcg
    12721 aaaagatatt cgatttgatg tagaagcggc cgtaatagtt gattcccagg aaatcctgcg
    12781 tgcctttgag gttttcaatc ggcttctcaa tgcttgtgtt ggtgaccttg agcacgccgg
    12841 tgctgattgc atcggtgaaa tcccagatga atgcctgttc gaccttgccg gcgatgatac
    12901 ggtccagcag tgcatagtta cggtagggtg agaactcccg cgtgtgttgc gtgacgccga
    12961 ccatggcctt ggggttggcc gcatggataa tgcggtaggc ttcggcgtgt gcccgcagca
    13021 acgcttccat aacagggacg acggattttt catcggggcg tttctcgagt ggggggaaaa
    13081 taccctgcat gtagcccgag tagatgtaga ccattggttc attgagcgtg caccacacgt
    13141 tcacctgttc tccccagcgg ccaacgacga agcgcacgaa tttcgtgaag tgctgcagcg
    13201 cgtcggcgcg ttcccagccg cgtttgccgt ctttctcttt gaagaaccag gccggcgagc
    13261 tgaagtggaa cagggttacg gatggctcga tattctgttt cttcagctcg gcaaacagcc
    13321 ggtcgtagaa tgccaccgcg ccggcatcgg gtttatccat tccctcacgg ggaaacaggc
    13381 ggtcccatgc gatcgagaaa cggtacgagt tagctcccat ttctttcatt aatgcgacat
    13441 cttcggcaac cttcagtgta tgcgcagttt tttgtttgat ga
//
LOCUS       DAOQJF010000041        13405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2244_length_13405_cov_4.702921, whole genome shotgun sequence.
ACCESSION   DAOQJF010000041 DAOQJF010000000
VERSION     DAOQJF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13405
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2244_length_13405_cov_4.702921"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PLF85_03665"
     CDS             complement(<1..736)
                     /locus_tag="PLF85_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18785.1"
                     /translation="MRAFIIVGIVALFAAHCSKPTIRELDGKIKGKASLRLADGSTIS
                     RDDSEEYHPQIVRLSDGYLVLVFGSDRTCSGCDPFAHNIFVAKSLTPYDGMFLPYFDT
                     PLPLRDELNNALNAPGAIDFAVVPNGAGVNVYFNDFNTSYQIKKANITDFVTVNPSWV
                     AIANSTYADHTVIGANAAGDRLVTTDFSGQAYIISPDVSVASDPYGFGLDNTSSAIQI
                     RQETSGSNDAYIASIYGGTMATTADYP"
     gene            complement(788..2248)
                     /locus_tag="PLF85_03670"
     CDS             complement(788..2248)
                     /locus_tag="PLF85_03670"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE18786.1"
                     /translation="MRSGFMLLLLATLLANCMSRPTERIDLAKARTSLRLEDGSTISL
                     PGYSDYSPVLVRSPSNTVILAFISNRPNAVAGYVSGNHYIYLTESVEPYFGFGELPAF
                     TAPVAVQHSGAPLNVVADSAIVATWIEGNLNLFVNRFAGITALSFASADISTGIVTFT
                     PDPLPNSARSSDMLLDARYKEAKIISRSGSNVYKSAIGTVDSGTPVTNPLLAGADSIS
                     PASSLASGYSDAYFIASGGQLYTGTLDSDFGEHPEFNAALASNGLYLTSVAQMQTYLG
                     AANPIVFSATDTSGEQDMYFVISHDATSLWLLTFNFGFAPEPNPGPYRVFVTQTAYDG
                     GFGSLYVADYYCSQDSRAPDLNVPYKAMLVDGSQRIASITANVGDGQTDWVFQPSASN
                     TQAASGAAIGTAGANGLLTFPLSAAFSSSAEVAWTGMNNSWVATGSDCQDWQSSMGSD
                     VGERGATNVTGSAVLQDTTASMCNNTFKLYCVEQPF"
     gene            2336..3118
                     /locus_tag="PLF85_03675"
     CDS             2336..3118
                     /locus_tag="PLF85_03675"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNE18787.1"
                     /translation="MTPLQAIILGIIEGVTEFLPISSTGHMILADHLMVVKDREFVKT
                     FEIAIQLGAIAAVFMLYIRRFLREMRLYFILGLAFLPTGIAGLLVYKHLKAHLFNPVS
                     VSIALIAGGVLLLFFDRLAEKHEHQPEGHATEIKPKTAIVIGLFQAISMIPGVSRAAA
                     TIGGGLVMGLNRVRAVEFSFLLAVPTMVAATGYDLYKQRESLNATHIDLLAIGAVVAF
                     AFALLAVRFFVGYISKRGFFVFGVYRIIVGVIFLAVFWGKSF"
     gene            complement(3125..>6565)
                     /locus_tag="PLF85_03680"
     CDS             complement(3125..>6565)
                     /locus_tag="PLF85_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNE18788.1"
                     /translation="IIARSRAGVQVKVAFDADVRDSDTGSLALQASGAFVVVATPGET
                     TQGQLLYGNNGTGVMRHNYCLADERYAWISTAPPDDTQMRRTPNIAIKVGSPQFGLAR
                     DFLRESSMFSQLLFGGGKAKTDYTTKFAALDQVIAAYWGPQESPMDVLGTELSEATRT
                     VDFYSTAFQATNTSSTKSYLDVPTVLKALETAKGVTIRKYFSSQALFDSASKAYTLTN
                     PSQYVNSNVRVGANIFVVDRNLSSARTYIYTGALRSQGNSSDDSVLLELRGPRVAQLV
                     AAYLDRIGGVAVPVSNSGDTAAAGQVVISEIIWMGSYSNTQTGDGADEFIELYNNTAS
                     AINISGWQFACTTDGTNNNSAITLPPGALIAPGGYFVVASKNTGAFAGSANHFDSKVG
                     ITNSSRECRLGNGKTAATVYGIANFGDVIDTIGNNTNGFDTAGWNRGVNDGSPNFIRK
                     SMERVNPSASGTANTNWRNNGYTFGLNTLVASNFRSNTFASPGASGTFTETAAAGNVV
                     INEILWMGSYDNGGTGQTDDEFIELYNNTASSIDISNWTISGTGISTITIPTGQTIAA
                     NGYYVIARNTSLAVSSANYSTTTAMSITNGGFQLELRTSLNTLIDTANNGGAPLAGVN
                     DATNLYRRSMERTTTGTDGTLAASWQSAFTAGSGIAAGYSSRTLATPGQVNSTIPADP
                     AVGDVVVNELMWMGSYDNAGTTQTDDEFIELWNKTASAINISFWQVNGTGINAVTLPP
                     GQTIPANGYYVIARNNLNAFTTANYYKSSTLGLSSTTLQVELRTSRNTVIDTANNGGA
                     PHAGLNDTTNQIRRSMERRPTATDGTLAASWLNSGVVGTNVAAGFSTRTIATPGQANS
                     FYPQTVTVVSSTSITVQYNLLPTAGTGAAGAENTANYTFTGGLTVSAASLAGNVVTLT
                     TSAQTPGASYTLTISNVTASGNSATLSPNTANVTGFVTMANLVINEVAPAETSSLDLI
                     ELYAVSGGSINGLEIFEGGTSLKVLPNITVATGDYIVIHINASGTDETSSKSQSGDVG
                     HIATAWDYWSADTGLTATDNAIILKSSSAVILDACVYGNNTGTWTGPTISTVIDPIIA
                     ASQWTKAGGTFAESDAVNNAGTILGGSSFRRTPNGNDTNNSAADWTSAAGLTIGSGNP
                     "
     assembly_gap    6566..6665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6666..7750)
                     /locus_tag="PLF85_03685"
     CDS             complement(<6666..7750)
                     /locus_tag="PLF85_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNE18789.1"
                     /translation="MEWLSKNAELIGILFMPVTYGFIGWLTNVVALKMMFYPLNFIGI
                     MPPYLGWQGIVPRKATGLALKVVNILSEKLIKVEDFFEKIPADKLAESYKPILREHIP
                     GMMHKVLEAVPAELKSKVAANENKIVSKAIAESEQRLVEITGHLKEDVSKVFSFKNMV
                     LRNLTGDRTHLLVDLFQEIGHKEFKFIGASGWYFGSLFGLAQMLIWYLYPGAWTLPVQ
                     GIIVGYVTNWLALIMIFRPMVEKRYLFFRYQGLFHRRQQEVSDKFSTMFADHVLNGKN
                     ILDEVLYRRISRTVVETMNEDIVQELRADGANSAAVDAFEKASDESRKQVAADLASRL
                     SQASETMEKLINRSMNVKATIYGRMKDL"
     gene            complement(8493..10529)
                     /locus_tag="PLF85_03690"
     CDS             complement(8493..10529)
                     /locus_tag="PLF85_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE18790.1"
                     /translation="MKFSKADAAFTAAMAAGIALLSGALYLHSRTRGAGSGDVIGKVY
                     YKREVAMRKFSDRMVWEDVDNGSPLYSHDAVMTGNYSDAELVLNSGLKLRLEANTLIE
                     LDLEGEGLKLKLAGGGIKATGAEGSRTTVTTTDGQAISMNEASASIRTSGEQTAVEVS
                     SGQVAVVNKEGSAQTVAKDEVLAAGQKSRVTLKLTGLVEDAIILAAGSNGRVSVSCSG
                     DAESAEFSKRSDMQSARTVKLAAGKATTQLPAGDWYTRCRGAGNAISAVRHFRILNAG
                     SYQVYRPDGDVKYSDKPELRFEFKAPRGVTKTRLEIAANARLENAVFDQVVSQSVAAV
                     VLPGPGRWYYRLTAAEDAGRIESQLPGSSGSFVMSKSPQAQTLSLISVAEPVFSVAQL
                     EAGKAFINYDGSGSYQYEIVPRTGGAPVASGNTAGGSIKLPPDLKGGNYALRIARESE
                     KVSQPFTVRERMTVQLQSPGDGSVIWVPAGSEGASLAVKWKASDEVNLYQLILAEDSD
                     LKRVLRRLNIDQDNFRFSGLKRGSYYLKVLALENGIPRAETKTVRLHVEDRLPPVAEV
                     FPKDDSRVDVTRTAGLQLRWQAVVGANSYEVRLLQKRRGELVPVQTLVTNKPTVVVSD
                     IRKFKEGEVVWEIRAQQADKSGKVLQKSEPVRNSLNVSFGATPPAPEIVPIIEE"
     gene            complement(10510..>10701)
                     /locus_tag="PLF85_03695"
     CDS             complement(10510..>10701)
                     /locus_tag="PLF85_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18791.1"
                     /translation="PMQKIKVKGKSQPQQIYAVIRKKSDKTGPKNLKELRKLLGIDMK
                     GKPTKSGDDEEVKYEILEG"
     assembly_gap    10702..10801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10802..12195)
                     /locus_tag="PLF85_03700"
     CDS             complement(<10802..12195)
                     /locus_tag="PLF85_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE18792.1"
                     /translation="MPLKNLPLRYKLMVITSGLIIVSLVVLSVISIRNFTQDKTDLIK
                     LQTNDTAKLIAEKLRTEISARVEGLQVFDRFAGFSGATAKSKKGARRKPAAAVTDRAN
                     LSAGSGVLRVSRYLFDAGEIRRGYSRDIVAEQQKVEIAPGAFARDEERLAERLKAVFR
                     GQTLLLTRTQSFGKPVWFIAFPVIEGEVAQRVITAYLSTEVLDSAFTSRAYTSFFVDS
                     DRALVVHSDKKETLAARNFKDHPAVEFLQGQETRTGFSIYRNNATKHDYFGAFSKLDI
                     GTTAVVVEMEKDRALESVNRLRITSALIAGIIILSALLLVYFFAKTISEPIRELKIAA
                     DKIKSGDYHLRLTPRTGDEVGALTQAFNDMAVGLEEREKLKGALGKFVNPEIAEKAMK
                     GEIKLGGERKTATIFFSDIRSFTAISEKLEPEEVVEFLNDYMTIMVRIIGQHQGIVDK
                     FIGDAIMAVWGVPES"
     gene            complement(12215..12547)
                     /locus_tag="PLF85_03705"
     CDS             complement(12215..12547)
                     /locus_tag="PLF85_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18793.1"
                     /translation="MPIPISQLWDNLMLFMVIGIFIELAVSAIFSVRIIDDLLNTTML
                     RSVKNALVLITAFGVCAKIDQLRFLYGTKIAIPELIHYILSSLVLARMANLVHDFINY
                     LKARSKAM"
     gene            12922..13374
                     /locus_tag="PLF85_03710"
     CDS             12922..13374
                     /locus_tag="PLF85_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNE18794.1"
                     /translation="MASPFESVLTAQRLPGELIRMALLAAPLSLMAAIKRAETPESLR
                     LRGIGGELVLGASDIVFAEQRNRKTFITCRTNDVQVSLNLRDLEKRLRPFGIVRIHKR
                     YLVNPAYVRSIQPQGSGRYLVRLADEEDTVLVAGRAYARKLREQLKQK"
BASE COUNT         2951 a         3585 c         3525 g         3144 t
ORIGIN      
        1 ggggatagtc tgcggtcgtt gccatagtcc cgccataaat ggatgcaatg tatgcgtcat
       61 tcgaaccaga agtctcctgg cgtatctgga tcgccgaact cgtattatca agaccaaagc
      121 catagggatc gctggccaca ctcacgtcag ggctgatgat ataagcctgt ccgctaaaat
      181 ccgtagtgac cagccgatcg ccggcagcgt ttgcgccgat gacggtgtga tcggcgtagg
      241 tagaattggc aatcgcaacc caacttgggt tcaccgtaac aaaatctgtg atatttgctt
      301 tcttgatctg gtacgaggta ttaaaatcgt tgaagtagac gttcacgccg gccccattgg
      361 gcacgacggc aaagtcaatg gcaccgggtg cgttcagtgc gttattcaat tcatcgcgca
      421 atggaagggg tgtatcaaag tatggtaaga acattccatc gtaaggcgtg agtgacttgg
      481 cgacaaagat attgtgcgca aacggatcgc aaccgctgca tgtacggtct gagccgaaga
      541 ccagcaccag atacccatca ctcagccgca cgatctgcgg gtggtactct tcagaatcgt
      601 cgcgcgaaat ggtcgacccg tcggcgaggc gtaaagaagc cttaccctta atcttgccgt
      661 cgagttcgcg gatagtgggc ttgctgcaat gggccgcgaa cagggccacg atgccgacga
      721 taataaaggc gcgcatggcg taggataaag gtctgatgcc gagcgtcaaa cgtaaattgt
      781 gggggtacta aaatggctgt tcgacgcagt acagcttgaa ggtattattg cacatcgaag
      841 ctgttgtatc ctgtagcact gcgctgccag ttacgttggt tgctccgcgc tcgccaacgt
      901 ccgaacccat actgctctgc cagtcctggc agtcgctgcc cgtcgccacc cagctgttat
      961 tcatgcctgt ccatgcgacc tcagcggaag agctgaacgc ggccgagagg ggaaatgtga
     1021 gtaggccgtt tgcgccggca gtgccgatgg ctgcacctga tgcggcctgg gtgttgctcg
     1081 cagacggctg aaagacccaa tcggtctgcc catcaccgac gttcgcggtt atcgatgcaa
     1141 tgcgctggct tccgtcgacc agcatcgcct tatatggcac attgagatcg ggagcgcgac
     1201 tgtcttgaga acagtagtaa tcagctacat aaaggctgcc aaaaccacca tcgtacgctg
     1261 tctgagtaac gaataccctg tagggacccg ggtttggttc cggggcaaat ccaaaattga
     1321 aggtgagtaa ccaaagcgag gtggcatcat ggctgatcac aaagtacatg tcctgttcgc
     1381 ctgaagtatc ggtcgcagaa aagacaatcg ggtttgccgc gcccaagtat gtttgcatct
     1441 gggcaacgct ggtcagataa aggccgttag aagccagggc ggcattgaac tccgggtgtt
     1501 cgccaaagtc tgagtcaagc gtgcctgtat acaactggcc accggaggcg ataaaatagg
     1561 catcactgta ccccgaggca agagagcttg ccggtgatat agaatccgcg cctgccagaa
     1621 ggggattcgt cacgggcgta cccgagtcta ctgtgccaat tgctgactta tagacattgc
     1681 tgcctgaacg ggatatgatt tttgcttcct tatagcgcgc atcgagtagc atgtcactac
     1741 tgcgcgcaga atttggcaat gggtcaggcg tgaaggttac gataccggtc gaaatatcag
     1801 ccgatgcaaa actcagggca gtaattcctg caaacctgtt gacgaacagg ttcaaattac
     1861 cttcaatcca agtggcgacg attgccgagt ctgcaacaac attcagcggc gcaccgctgt
     1921 gctgcacagc gaccggagcc gtaaaggccg gcaactcgcc aaaaccaaag tagggctcaa
     1981 ctgactctgt caggtatatg taatgattgc ccgaaacata acccgccacg gcgtttggac
     2041 ggttggaaat aaatgccagt atgacagtgt tgcttgggct gcggaccaat acaggcgaat
     2101 aatcgctgta gccaggaagc gagatcgtcg aaccgtcttc aaggcgcagg ctggtacgcg
     2161 cttttgccag gtctatgcgc tcagtcggac gcgacataca attggccagc agcgtcgcca
     2221 gcagtaggag cataaagcct gagcgcatgc tctctgaaga atagaccttt gatacgccgt
     2281 caaactctct gctattcaac ttgaccccct gatttctcac ccccaacgcc cacccatgac
     2341 cccgctgcag gcaatcattc tcggcatcat cgaaggagtt accgagtttc tcccgatctc
     2401 ttctaccggc cacatgattc tcgccgacca cctgatggtt gttaaagacc gggagtttgt
     2461 caagaccttc gaaatcgcca tccagctggg cgcgattgcc gccgtattca tgctctacat
     2521 ccgccgcttc ttgcgcgaga tgcgtctgta ctttattttg gggctcgcgt ttctacctac
     2581 cggcattgca gggctgctgg tttacaaaca cctcaaagcg cacctcttta acccggtttc
     2641 ggtctcgatt gcgctcatcg ccgggggagt tctgctcctg ttttttgacc gcctcgcgga
     2701 aaaacatgag caccagcctg aggggcacgc caccgagatt aagcccaaaa ccgcaattgt
     2761 gatcggtctc tttcaggcga tttcgatgat tcccggtgtc tcgcgcgccg cggcgaccat
     2821 cggcggcggt ctcgtgatgg gcctcaaccg cgtgcgcgcc gtcgagttct cgttcttgct
     2881 cgcagtaccg acaatggtgg ctgctacggg ttacgatctt tataagcagc gtgaatcgct
     2941 gaatgcgacg catatcgatc tgctcgcgat cggcgccgtg gtcgcattcg ctttcgcgct
     3001 actggcagta cgtttctttg tgggctacat ttcaaagcgg ggatttttcg tcttcggcgt
     3061 gtaccgcatc attgtcggcg tgatttttct cgcggtgttt tggggtaaga gtttctgata
     3121 gccgttaggg gtttcccgaa ccaatcgtca ggccggcagc agaagtccag tccgcggctg
     3181 agttgttggt atcattgcca ttgggcgtac gacggaatga tgaccctcct agtattgtac
     3241 ctgcattgtt gacagcgtct gattctgcga atgttccccc ggctttggtc cattgactcg
     3301 ctgcaataat tggatcgatt actgtgctaa ttgtcggccc agtccaggtt ccggtattat
     3361 taccgtaaac acaagcatca aggattacag cacttgagct cttgagaatg atggcattgt
     3421 cggttgccgt caggccggta tcagccgacc agtaatccca cgcagttgca atgtgtccta
     3481 catcacctga ttgactctta gaagaagttt catccgtacc ggacgcatta atatgaataa
     3541 caatataatc tcctgttgcg accgtgatat tgggcagaac tttaagcgat gtgccgcctt
     3601 cgaagatttc caggccgttg atgctgccgc cggaaacggc gtataactcg atcagatcca
     3661 ggctagatgt ttctgccggc gcaacttcat ttattacgag gttcgccatc gtcacaaacc
     3721 ccgtcacgtt cgccgtgttc ggcgaaagcg tcgcgctgtt accactcgcg gtcacgttgc
     3781 tgatggtgag agtatagctg gcccccggcg tctgggcact tgtggtgagc gtcaccacat
     3841 tgccagcgag agaagctgct gagacggtaa gccctcccgt gaacgtgtag ttcgccgtat
     3901 tttctgcacc ggcagctcca gtgcccgccg tgggtaacag gttatactgt acggtgatgc
     3961 tcgtcgatga gactactgtg accgtctgcg ggtagaatga gtttgcctgt cccggagtcg
     4021 caatggttct tgtgctgaaa cccgccgcca cgttcgtgcc gacgacaccc gaattcagcc
     4081 agctcgcggc cagtgtaccg tcggtcgccg ttggccgacg ctccatcgaa cggcgaatct
     4141 ggttggtcgt gtcattcaat ccggcatggg gagcgccccc attatttgca gtatctatca
     4201 ctgtattgcg actggttcgc aactcaacct gtagcgtagt cgaagagaga ccaagagtag
     4261 agcttttgta gtaattcgct gtagtgaagg cattgagatt attgcgcgcg atcacataat
     4321 agccgttggc aggtattgtc tggcccggcg gcagggtgac ggcattgatg cctgtgccat
     4381 taacctgcca gaagctgata ttgatcgccg aggctgtctt gttccacagt tcgatgaatt
     4441 cgtcgtcggt ctgcgtcgta ccggcattgt catagctccc catccacatc aattcgttga
     4501 ctacaacatc gcccacggcc gggtcggcag gtatggtcga attcacctgc ccgggtgtcg
     4561 cgagcgtgcg gctgctgtaa cccgcggcga taccagaccc tgcggtgaaa gcgctttgcc
     4621 aactcgccgc cagtgtgcca tcggtgcccg tcgtcgtgcg ctccatcgag cgtcggtaaa
     4681 ggttagtcgc gtcattgacc ccggcaaggg gagctccccc gttgttagcc gtatcaatca
     4741 gcgtgttcag gcttgtgcgc agttccagct gaaagccgcc gttcgtgatg ctcatggcag
     4801 ttgtggtcga atagtttgca gagcttacgg ctaaactcgt gttgcgtgcg atgacatagt
     4861 atccattggc ggcaatcgtc tgcccggtgg ggattgtgat cgtgctgatg ccagtgcccg
     4921 aaatggtcca gttactgata tcgatgctgc tcgccgtgtt gttatatagc tcgatgaatt
     4981 catcgtcagt ctggcccgtg ccgccattat cataactacc catccagaga atctcgttga
     5041 tgacaacatt gccggcagca gcggtctcgg taaaggtacc gctcgccccg gggcttgcga
     5101 acgtgttgct gcggaagttg gaagcaacca gggtattcag gccaaaggtg tagccattgt
     5161 tgcgccagtt tgtattggcc gtgccggaag ctgaagggtt aacgcgctcc atgctcttgc
     5221 ggatgaaatt gggactacca tcattgacac cccggttcca gcccgcggta tcaaaaccgt
     5281 tggtgttgtt gccgatggta tcgatcacat cgccgaaatt ggcaatgcca tagacagtcg
     5341 cggcggtctt gccgttgccc aacctgcatt cgcgggatga gttggtgata ccaaccttgc
     5401 tgtcgaaatg gttggcgctg ccggcaaatg cgccggtgtt cttagaggcc accacaaagt
     5461 aacctcccgg cgcgatcaac gcccctggcg gtaaggtgat agcggaatta ttgttggtgc
     5521 catcggtggt gcaggcaaac tgccagccgc tgatgttgat cgcgctggcc gtgttattgt
     5581 agagctcgat gaactcgtcg gcaccatcgc cggtctgcgt attgctgtaa ctgcccatcc
     5641 agataatttc gctgatgacg acctgccctg ccgctgccgt gtcgccgctg ttgctgaccg
     5701 gcaccgccac accgccaata cgatcgagat acgcggcaac cagctgcgcc acgcgcgggc
     5761 cacgcaattc aagcagaaca ctgtcgtctg aagaattgcc ctgcgagcgc agcgcacctg
     5821 tatagatgta ggttctggcc gacgacaggt tgcggtcgac gacgaagata ttagctccca
     5881 cacgcacatt gctgttcacg tactgcgaag ggtttgtcag tgtgtaagcc ttcgaagccg
     5941 agtcgaagag cgcctggctt gagaagtatt tgcgaatcgt cacgcctttc gctgtttcaa
     6001 gcgctttgag cactgtaggc acatcgaggt acgatttggt ggagctcgtg ttcgtcgcct
     6061 ggaaggccgt agaataaaaa tcgaccgtgc gcgtcgcttc tgaaagttct gtacccagaa
     6121 catccatggg tgattcctgc gggccccagt aagcggcaat aacctggtca agagctgcga
     6181 acttcgtggt ataatcagtc ttcgctttac ctcccccgaa gagcagttgc gaaaacatac
     6241 tcgattcacg taagaaatcg cgtgcgagac cgaactgcgg gctgccgacc ttgatcgcga
     6301 tgttgggtgt gcgcctcatc tgcgtgtcat cggggggcgc agtcgagatc caggcataac
     6361 gctcgtcggc gaggcagtag ttatggcgca tgaccccggt tccgttgttg ccgtacagta
     6421 actgcccctg cgtcgtttcg ccgggagtag ccacaaccac aaaggcacca ctcgcctgta
     6481 gagccaaaga accggtatcg ctgtcgcgca cgtcggcatc aaaggcaacc ttcacctgca
     6541 cacccgcgcg cgaacgcgca atgatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnaggtc tttcatgcgg ccgtagatcg tcgccttcac gttcatcgaa cggttgatga
     6721 gtttctccat tgtctccgag gcctgcgaca ggcgagaggc aaggtcagct gcgacctgct
     6781 tgcggctttc gtcgctcgct ttttcaaagg catcgaccgc agcagagttt gcgccatctg
     6841 cgcgcagctc ctgcacaata tcttcgttca tcgtttcgac aacggtgcga ctgatacgcc
     6901 ggtatagaac ttcatcgaga atatttttgc cgttgagcac gtggtcggca aacatcgtcg
     6961 aaaatttgtc agatacttcc tgctggcggc ggtgaaagag accctggtac ctgaagaaca
     7021 aataacgctt ctctaccatt ggacgaaaaa tcatgatcag ggcgagccag ttagtgacat
     7081 agcccacgat gataccctgc acgggcaggg tccatgcacc tggatacaga taccagatca
     7141 gcatctgcgc caaaccgaac aagctgccga aataccagcc cgaagctcca atgaatttga
     7201 attctttgtg acctatttcc tgaaacaggt cgaccagcaa gtgtgtgcgg tcacccgtca
     7261 ggttgcgcaa caccatgttc ttgaagctaa agaccttgct gacatcttct ttcaggtggc
     7321 cggtgatctc taccaggcgt tgctctgatt cggcgattgc cttggagaca attttgttct
     7381 cgttcgcagc gactttactc ttaagctccg ccggaacagc ttcaagcacc ttgtgcatca
     7441 tgcccggtat atgttcgcgg agtatcggct tatacgattc ggccagcttg tcagcaggta
     7501 tcttctcgaa gaagtcttca accttgatga gcttttcact gagaatgttc acgactttga
     7561 gcgcgaggcc agtcgccttg cgggggacga taccctgcca gccgaggtac ggaggcatga
     7621 taccgataaa gttaagcggg taaaacatca tcttgagcgc cacgacgttc gtgagccagc
     7681 cgataaagcc gtacgttacc ggcataaaga ggataccgat cagctcggca ttcttgctca
     7741 accattccat ggttaagagt gcaaagtggc gacagagcga aaagtgaatc tttgttcaca
     7801 aagattcacc gagggtagtg agaaaaaatc gcagtttgct ggtatttaat atttagaatg
     7861 cagaatattt cctccacagg ctgctaccgt acatcagtgg cattacggcc tgaatcgtac
     7921 cgtcgtatgc gcgcagccct taagatcctg aggcgcaatg gggtcagatg gtcagaagca
     7981 cagctactga gccgattggg cagaacatac ctggttagat ggaaaggatc tggtttgaag
     8041 gcggcatcaa cccggcgctg cggggccagg ttatcgaggc aacggattcg acgaccttgg
     8101 tatgtggagg aagttctcta cgccctctta tggcagagaa gcgtacattc gggtcagtct
     8161 gtgtccagaa tggttgattt ttcgatacgt gtttatctgc cgcgtattct tgaggagttg
     8221 ctggccaacc ccgtgcgcaa ttgcacccgc agcgagcgga actcgcgcta ttggtcccag
     8281 cgtcaggccg agcgaaaacc tgtccaccgt ttcaggttga ttacttattc atgcaagact
     8341 gagaagaatc acctaggtga gctggaatac aggcagacag ctaaatttct gacaccagag
     8401 acaagtttca ccccacttgc ccgacctttt caacctactt catagcctga aacctgtgtc
     8461 acacggataa caccggggct gaaaaaacca atctactctt caataatggg gacaatttct
     8521 ggcgcaggtg gtgttgcgcc gaacgaaacg ttcaaactat tgcgcacggg ttcgcttttc
     8581 tgcagaacct taccggattt gtccgcttgc tgcgcacgaa tttcccagac aacctctcct
     8641 tctttgaatt tacggatatc actcacaacg actgtcggct tattcgtcac caaagtttgc
     8701 acaggcacaa gctcacctct gcgcttctgc aagaggcgaa cttcatatga gttggctccc
     8761 accactgcct gccagcgcaa ctgtaacccg gccgtgcggg tcacgtcaac cctggaatca
     8821 tccttcggaa aaacttcggc gaccggtggc aggcggtctt caacgtgcag ccgtactgtt
     8881 tttgtttcgg ctcgcggaat tccattctcg agagcgagaa ctttgaggta ataactcccc
     8941 cgcttgagac cggaaaaacg aaaattatcc tggtcgatat tgagtctgcg aagcacgcgt
     9001 ttcaggtcgc tgtcttcggc aaggatcaac tggtacaaat tcacttcgtc gctcgccttc
     9061 catttcactg ctaacgatgc gccctcactt ccggccggca cccagatcac cgagccatcg
     9121 ccaggtgact gcagctggac ggtcatacgt tcacgcacgg taaaaggctg gctcaccttc
     9181 tccgattcgc gagcgatacg cagagcgtaa ttacctcctt ttaggtctgg tgggagctta
     9241 atcgaacctc cggctgtatt gccactggca accggcgctc caccggtgcg aggtacaatt
     9301 tcatattgat aactgccaga accatcgtag ttaatgaaag ccttgcctgc ttcgagctgc
     9361 gccacagaaa ataccggttc agcgacagag atcagtgaca gcgtttgggc ctggggagat
     9421 ttagacatga caaagctgcc cgaagaacct ggtaactgtg attctatacg accagcatct
     9481 tcagcagctg tcagccgata gtaccagcga ccaggaccgg gtaatacaac agccgcaacc
     9541 gactggctca cgacctgatc gaacacagca ttttccagcc gcgcgttagc cgcaatctcg
     9601 agacgggttt ttgttacgcc gcgcggggct ttgaactcaa agcgcaactc tggtttgtcg
     9661 ctgtacttca catcgccatc gggacgatac acctggtaag aacctgcgtt cagaatacga
     9721 aaatgcctga ctgccgaaat cgcattgccg gcaccgcggc aacgcgtgta ccagtcgccg
     9781 gcaggtaatt gcgtcgtggc tttgcccgct gcgagcttca ccgtgcgcgc agactgcatg
     9841 tcgctccgtt tcgaaaactc tgcgctttct gcatcacctg aacaactcac cgaaacgcgc
     9901 ccattactac cggccgccag gataattgcg tcttcaacga ggcccgtaag tttgagtgtg
     9961 actcgcgatt tctgtccagc agcgagaacc tcgtcttttg cgacggtctg tgcagaaccc
    10021 tctttgttga ctaccgcaac ctggcctgaa ctcacctcga cagctgtctg ttcaccgctc
    10081 gtacgaatac tcgccgatgc ttcgttcatg ctgatggcct gaccatcggt cgtggtgacc
    10141 gtagtgcgcg aaccttctgc gcctgtagcc ttaatgccgc cgcctgcaag cttcagctta
    10201 agcccctcac cttcgagatc gagctcgata agggtattgg cctcgagcct cagcttcagg
    10261 cctgagttga gtaccagctc ggcatcagaa taattccccg tcatgaccgc atcgtgcgaa
    10321 taaagcggtg agccgttatc aacgtcttcc cagaccatgc ggtcagagaa cttgcgcatg
    10381 gcaacttcgc gcttgtaata gaccttgccg ataacatcgc cgctgcccgc accacgcgtg
    10441 cggctatgca ggtagagtgc gcccgaaagc agggcgatac ctgcagccat agccgccgtg
    10501 aaagccgcgt cagccttcga gaatttcata tttcacttct tcatcgtcac ccgactttgt
    10561 gggcttgccc ttcatgtcga taccgagcag cttgcgcagt tctttcagat ttttcggccc
    10621 cgttttgtcg gattttttgc ggataacagc atagatttgc tgcggctggg attttccttt
    10681 aaccttgatc ttttgcatcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 ngattcggga actccccaca cggccataat ggcgtcgccg atgaacttat cgacaatacc
    10861 ctggtgctgg ccaatgatgc gcaccataat ggtcatgtag tcgttaagaa actcaaccac
    10921 ttcttctggt tcaagctttt cagagatcgc cgtgaatgaa cgaatgtcgg agaaaaatat
    10981 cgtcgctgtt ttgcgctctc cgccgagctt aatctcaccc ttcatggcct tttcagcgat
    11041 ttcaggattc acaaatttcc ccagcgcccc cttgagtttt tcgcgctctt cgagaccgac
    11101 agccatgtcg ttgaaggcct gcgtcagagc accaacctca tcgcctgtac gaggggtcag
    11161 tctcaggtgg tagtccccac tcttgatctt gtctgcagca atcttgagtt cgcgaatggg
    11221 ttcagaaatc gttttggcaa aaaaatacac caggagcagt gcagaaagaa tgattatacc
    11281 cgcaatcagg gccgaggtga tgcgcaaccg gttcaccgat tcgagggcgc ggtctttctc
    11341 catttcaacg accactgccg tggtgccgat atccaatttg gagaatgccc cgaagtaatc
    11401 atgcttcgtg gcattgttgc ggtaaatact gaaaccagtc cgcgtttcct gtccctgcaa
    11461 aaactccacg gccggatggt ctttgaagtt gcgggctgca agggtttctt tcttatcgct
    11521 gtgcacaaca agggcccggt cgctgtcgac gaaaaatgac gtataagcgc gtgaagtaaa
    11581 ggcagaatca aggacctctg tcgaaaggta cgcggtgatg acacgctgtg ccacctcacc
    11641 ctcaatcacg gggaacgcaa taaaccacac cggtttgcca aatgactggg tacgggtcag
    11701 cagaagagtc tgccccctga aaacagcttt cagtctttct gccaaacgtt cttcgtcgcg
    11761 cgcaaaagcg ccgggggcaa tttctacctt ctgctgttct gccacgatat cgcgggaata
    11821 tccacgccga atttcacctg catcaaaaag atagcggctc acacgcagca caccggaacc
    11881 cgccgagaga tttgccctgt ctgtcacggc ggcagccggc ttgcgacgcg cgcctttttt
    11941 acttttagcg gtggcgcctg aaaaaccggc aaaccgatca aagacctgca gcccctcgac
    12001 cctggcgctg atttcggtcc tgagcttttc agcgatcagt tttgcggtat cattagtctg
    12061 cagcttgatc aggtcagtct tgtcctgtgt aaaattgcgg atagaaatta cggaaagcac
    12121 aacaagactg acgatgatca accccgaggt gatcaccatc agtttgtagc ggagcggcag
    12181 gtttttgagc ggcatgcaga agaaagaaat agctctacat cgccttactg cgcgctttca
    12241 ggtaattgat gaaatcatgc accaggtttg ccatgcgcgc cagtacgaga ctcgagagaa
    12301 tgtaatggat gagctctggt atcgcaatct ttgtcccata aaggaagcgg agctggtcaa
    12361 tcttggcgca gacaccaaaa gcagtaatga ggacgagtgc gttttttacc gaacgcagca
    12421 tcgtcgtgtt gagtaggtca tcgatgatcc gcacggagaa aatcgcagaa actgcaagct
    12481 caataaagat accgatgacc atgaacagca tcaggttatc ccacagttgc gaaatgggta
    12541 ttggcatgca gcaggctttc tgtcgagcca tgcaacggaa agtaaaaaag acaatggcgc
    12601 gttacggatg ccaaacatgg tttacgaatc aacacttgta tccgtttcag atgtagcggc
    12661 atgttgttct ataggtttcg ctggcgcatt ctagcgttgg ccgtgctgat atttgcgacc
    12721 agcaactttc tcaggtcctc ggtcgccgag catgggccct atctggtctt ggcccgtgag
    12781 ctgatttcgg cgttctcgcc agccctggcg gggtatgtgg ctgcgcttgt cttgcgtggc
    12841 cgactttcgt tacctatgaa ctcgctgatt atttttttct cagcaatttc tggtttcatg
    12901 gtcggctagc tcttactcga catggcaagt cccttcgagt cggtgcttac agcacagcgg
    12961 ctgccgggcg aacttattcg gatggcactg ctcgctgctc cactctctct gatggcagca
    13021 ataaaaagag cagaaacacc tgaatcttta cgtctgcgcg gtattggcgg cgaattggtg
    13081 ctaggggcct ctgacattgt ctttgccgaa cagcgaaacc gcaagacgtt tatcacctgc
    13141 cgcaccaatg atgtgcaggt ttctctgaac ctgcgtgacc tcgaaaaacg gctgcgacct
    13201 tttggcatcg tacgtataca caagcgatac ctggtgaatc cggcgtacgt gcgcagcatt
    13261 caaccccagg gaagcggcag gtatctggtt cgcctggccg atgaagaaga caccgtgcta
    13321 gttgctggcc gggcctatgc gcggaagctg cgcgaacaac tcaaacagaa ataaacagtg
    13381 ccgttgctgc attttttagt gccgg
//
LOCUS       DAOQJF010000042        13330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2271_length_13330_cov_5.001281, whole genome shotgun sequence.
ACCESSION   DAOQJF010000042 DAOQJF010000000
VERSION     DAOQJF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2271_length_13330_cov_5.001281"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /gene="dapB"
                     /locus_tag="PLF85_03715"
     CDS             <1..736
                     /gene="dapB"
                     /locus_tag="PLF85_03715"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNE18795.1"
                     /translation="GLIPSLQLTMPIEFAQHPDLGKIYRAVTGISFDAEIVPLAKAKI
                     PQGGLIDFSHASTVIETLRKAAETGSPVVVGTTGFTPEQRKALEAMAEKIPLIIASNM
                     AIGVNVLFALVEQAARALAPHGFDPEVMEIHHNLKKDSPSGTAVTIKEALLRGYGFSE
                     DNVLYGREGMIGERKKEELGVMALRGGDVVGDHTAYFLGQGERIEIKHQATNRDTFAA
                     GALAALKFLQGKKPGLYSMKDVLGLG"
     gene            736..2694
                     /locus_tag="PLF85_03720"
     CDS             736..2694
                     /locus_tag="PLF85_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE18796.1"
                     /translation="MRHLRWLLLLLSSALACAVSCSGGKGARELKAVGGVLDLSQHDF
                     HSEPVITLGGEWLYYPDQLLTSAAEAKTVQGVPVIIPQTWSGYGFGTYHLRLTLPDAH
                     EIFKLHMEAQLSAYDVYISGVRRAASGLPGKTMAETKPATEPLSAVLGAAGEYDIFLR
                     VANFHHRKGGVFKKIQAGRAAALDHLVYQKRFLDILLVGVLLMSGIYYFIVFLFRPRN
                     KAELFFAAVCFMIMGRTVTTGEKILLLSWPEAPFAFYTMVEYLSYFWGIPFALSFFSA
                     LYGRLVHRFLLRGFYISSVVFSLPVVLLPLRLYSHTAYLYIPVLVAGLIVVVLILGRA
                     LLQGLPSAGLLAGGFSVLALAVMNDAFNTLELLRTGYLVQYGFMAIVVCYSAVLAARF
                     SQALESSERAEQALREANETLEARVKERTQELESAKDVAEEANRQKDNFLAVVSHDLR
                     SPLSGILSALQAMGLSASGGELAAAAEKSAVRMLKTIDSLLAVRRIGNAPRPVDAEVL
                     PGRIAGEILSEMEAEAAKKKIRLLNELPTHWAIPSNPEVLRHVLRNLLSNAVKFTPAG
                     GCVLIYRPDGDRAAITVRDEGIGIGPEDIAAILGSKKITPRPGVSGEKSSGLGLSLCR
                     ELLAREGARLEIVSEPGKGSAFTIVWSD"
     gene            2823..4148
                     /gene="murA"
                     /locus_tag="PLF85_03725"
     CDS             2823..4148
                     /gene="murA"
                     /locus_tag="PLF85_03725"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNE18797.1"
                     /translation="MSEFHIEGGHKIGGTITAQGNKNEALPVLCAALMNPQGVIIRNV
                     PQIEDIKNLLKIIEAVGANIRTLEDGHSYEITTAEIKMSELPTELVAGLRGSITLLGP
                     LLARAKKVFLPRPGGDKIGRRRVDTHLLALSALGAKVEVFNDGYMLEAEKLKGTAILL
                     DEASVTGTENAVMAAACAEGVTLIENAASEPHVQGLCRFLVAQGVKIEGIGSNMLKIH
                     GVGGFENLKAADHTIGPDYLEIGSFVAMSAMTGGDLVVQDVNPIDLRMIKFVFERVGI
                     NWRYHEREGKGAAGNNHIVDLVMKSPYALKIQTDVHGEIPKIDDAPWPMFPADLISII
                     LTVATQCTGTVLIHEKLFESRLYFTDKLMGMGAQIVLCDPHRAVIIGPSKLYGSRMTS
                     PDIRAGMALVIAALAAEGTSVIQNIVQVDRGYENIDARLKTLGARIERV"
     gene            4190..5533
                     /gene="dnaB"
                     /locus_tag="PLF85_03730"
     CDS             4190..5533
                     /gene="dnaB"
                     /locus_tag="PLF85_03730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNE18798.1"
                     /translation="MQLFDEEAEQVVLGSVMQTPSLFEEVAQVLKPEDFYYEGHRIIY
                     EAIASEIDRGEQPNAILIINALKNQNMLERAGNRAGIMNIASLGVLVGINTFAEKIRE
                     LSLRRRLIISFRELEKKAGDTGFKLDTVLTDTEGALTHLADRYSSYNVRHIKDTNKEF
                     LEFLKHVRESRDGITGTATGFKKLDELTGGFKPGQMIVIAARPGMGKTTFALNIALNV
                     CMNKARPMAVVFFSLEMTRLELLLRLLSARAFLDSQKVQKGRINDKEMARLAHTAKEI
                     YESEFYIDDSADLTTWEFKQRARRLGRTIKQQGKELGLIVVDYLQLMTDRDRAQDGRQ
                     NEVAAISRAIKLIAKDLGVPILALSQMNRSIEQRGKDPRPQLSDLRESGAIEQDADMV
                     VFIHREQGKDIPEDMKNIAEIIIGKNRGGPRDDVKLAFLSDKSLFTDYEPAAPAE"
     gene            complement(5550..6641)
                     /gene="dinB"
                     /locus_tag="PLF85_03735"
     CDS             complement(5550..6641)
                     /gene="dinB"
                     /locus_tag="PLF85_03735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNE18799.1"
                     /translation="MSTGFRTIVHVDMDAFFTSVEVLDHPELRGKPVVVGADPKAGHL
                     RGVVSAASYEARKFGVRSAQPVSTAYKLCPEAIFMPHRFERYRAVSDAVMEVLAEFSD
                     RLLRMSIDEAVLDCSETGAKYGTWKNLGAKIKEAIRSKIGLTCTIGIASGKTLAKMAT
                     EIKKPDGLTLVHPGKEREFLAPLPVEAIPGVGPRAKETLNRLGLRKIGDLQTAQADQL
                     GAELGKWAQRLLAIANGIDDDEVETTHERKSFGEERTNQADLENLSQVQAAFTEIADD
                     LARRLASKNTSGRTITIKVRFADFHTITRSQTIPVPARGSQTLFETAWRLFTQNVSEE
                     KLAKEKIRLLGIQLSNLYTAKPGEQLWLF"
     gene            6727..7152
                     /locus_tag="PLF85_03740"
     CDS             6727..7152
                     /locus_tag="PLF85_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18800.1"
                     /translation="MKRGIFLSFFACLLIIPACSSTPKPIPLVQTRALAAANPGLGAL
                     EQQIATKKAEQDVLEQRQSELTEKSAQSPKDENLKRQLDRLEKENALCQAELAALIGR
                     HQLQSARILQRSDIAAHEKAVAKLDEAVADRRNALQGGK"
     gene            7152..8216
                     /locus_tag="PLF85_03745"
     CDS             7152..8216
                     /locus_tag="PLF85_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE18801.1"
                     /translation="MRWLSIVLIAAIAPLYADLFYYPSEYSKLQNDIYALGIQKSILE
                     AQLANEKKDAAFQKNRFEEKIRNLESRIASLEDKLRATENALRDERRGRVEDNAYSSL
                     RLDEKDRQIRALRRESSDTQEKMAADAAAQEKKYHAEIQKLKAELEQNREEFRKQLAE
                     LTEQKNKRIAELEAALSQKSRSLAECLDLAQQQKQTVSELETQAKELEDKLQKEIAEG
                     NLRIKRFKDRIVINIDDKILFPSGSAQLRREVKKTLDTVADMLARNKGSRVLIEGHTD
                     NVPIKTPRFPDNWELSSARALSVLRHLLETKHLEPARFGAAGYGEYRPVAPNDTAANR
                     QLNRRVDIVLQLLEAQGGDK"
     gene            8213..9265
                     /locus_tag="PLF85_03750"
     CDS             8213..9265
                     /locus_tag="PLF85_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18802.1"
                     /translation="MSWRGVIALTSLFLSTGCGDRPGKAASSLHLAVDAEIAQLLPGL
                     EALQFEGPAVGETLAKLNSSYEEWCAAPGKATGLMRDLTEQERKALKADDFALRTRLG
                     CSAWYALTDQSAERDFAYTRNQIDAGLRQSANCVSVGFMQPYLMAVQLDCAQLTVVDL
                     GFRTLYLHAVLLPLLTAADFAGIDSVLTQFEKTTGVSFNAVCESSGVARCKIALMAFA
                     EKYRSRLKATLLLSPLAKFRAEVGSMPTVIYMSNVPDPHFTSAEEFTELQKNLAGSGD
                     KLYAIYHQAESTNFGVYELASGKIRTVCADAFVVSQAGRYSKDRCTYYPPSRRAFTTY
                     FDNASENKAEKKVCQF"
     gene            complement(9273..10103)
                     /locus_tag="PLF85_03755"
     CDS             complement(9273..10103)
                     /locus_tag="PLF85_03755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE18803.1"
                     /translation="MSAYAAGATRGQVYIRSLSHSDPEIKQMRSEVTENLRAIADGQA
                     PEIKWRRYRLSDKDTFFKVMARTVLNHDTISSVNRLASLWDLNKGDEWLLPNVRGIAV
                     YGERQSVAKKFNRELDELIAVPGQDNFWFITGLHFDPTEKEFFNLKAFIHPVDGRITS
                     GFGVRRDPFTEKHKFHKGIDIACSIGTPVVASAEGTVVFAGSKKGYGKTIIVEHRNGY
                     RTLYGHLSKYSVKAGTKVKQGQKIALSGMTGRATGPHLHFEVRRQGQPERPNFHLAKI
                     "
     gene            10280..11140
                     /gene="prmC"
                     /locus_tag="PLF85_03760"
     CDS             10280..11140
                     /gene="prmC"
                     /locus_tag="PLF85_03760"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNE18804.1"
                     /translation="MSNLPPTLIETLRKAEEYFAAKKIPSPRLEAQLLFAHFLKLRRI
                     DLFTQPERPLIAAELESLRDAMRRKVAGQPTAYILGSRDFFGRSFVVTPAVLIPRPET
                     EELVEHVLSLKPAARHVVDAGSGSGCIGITLAAELKAAELTLLDISSKALQVAAANAA
                     QLLSETGTQVHTLCADFCAGELSLSQQAELIVSNPPYVLPEEFAALDASVRDHEPRLA
                     LVAEKFEQLHRGLVAFAWRNLAPGGILALETHPQKSSEVAEWVGEAGFVSVEIRNDLA
                     ARPHFVLAQK"
     gene            11168..12067
                     /locus_tag="PLF85_03765"
     CDS             11168..12067
                     /locus_tag="PLF85_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18805.1"
                     /translation="MKSRHIITFVVILTFLTFYFIFDNVFSIWEQASYTRFRPVTTGL
                     PSAQIFRDVQVGDATADFFVAPAGSARRPLLVVMHGGFLQGGNKKNYAYLGGLGVRQG
                     FVVAVLQLPNYPGILSRPFFSAETLHARALPEQSRRFARFVTNVAALADRFNFDAKKL
                     HVMAHASGALLIGEADLSQFKSVTLVSPILSLKDNVAKIAPMQLRAVDGYIKDADALK
                     LSPAEWLPRTKMPVFLLCTERDLPYIKDACKELPLARPGAAAVERVLVEKPSHFELMF
                     HLGSKIEEATDPLKKFWFINSRL"
     gene            complement(12083..12490)
                     /locus_tag="PLF85_03770"
     CDS             complement(12083..12490)
                     /locus_tag="PLF85_03770"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNE18806.1"
                     /translation="MKALTKQTITHLREQAVKASRQAYAPYSQIQVGAALLANDGNIY
                     LGCNVENASYGLTICAERSAVSAAMQAISDERERLIRAIYVVNNKLPEIPPCGACRQV
                     IFEFGKGGIVFFDGKKGMKKILIDKILPEAFRL"
     gene            12633..12923
                     /locus_tag="PLF85_03775"
     CDS             12633..12923
                     /locus_tag="PLF85_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18807.1"
                     /translation="MYQSTISHSKAVFSSLIDQVKAGETVMITDRDVPVALLTPIPRE
                     NYKGSELLADLERQGLLRRATAAPCAVLDDLPPKNQLTGALAALLEERREGR"
     gene            12920..>13330
                     /locus_tag="PLF85_03780"
     CDS             12920..>13330
                     /locus_tag="PLF85_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE18808.1"
                     /translation="MIFWDSSAVVPLIVREASSEKLLQIYQSDPEMLVWWATELECVS
                     AICRRERDANVHAGHASSAFGRLKRLAETWHEVQAGDRVKATARRLLRTHNLRTADAL
                     QLAAAVTISQGDSSRITFLTLDDRLRVAAEREGLA"
BASE COUNT         2861 a         3724 c         3808 g         2937 t
ORIGIN      
        1 gggattaatc ccgtctctac agttaacaat gcccattgaa ttcgcgcagc accctgacct
       61 gggaaaaatc taccgtgcgg tcacgggaat aagttttgat gcagagattg taccgctggc
      121 aaaagcaaag atccctcaag ggggattgat tgatttttcg catgcatcga ccgtcattga
      181 aacattgcgc aaagcggcag agactggctc gccggtagtt gttggcacaa ccgggtttac
      241 gcccgagcag cgcaaggcgc tcgaagccat ggccgaaaag attccgctca ttattgcttc
      301 gaacatggcg attggggtga acgtgctgtt cgcgctggtc gagcaggcag cgcgggcact
      361 cgcgccccac gggtttgacc cggaagtgat ggagatccac cacaacctca aaaaagattc
      421 accttcgggc actgcagtca ctattaaaga ggcattgctg cgcggttatg gctttagcga
      481 agacaatgtg ctctatggtc gcgaaggcat gatcggtgaa cgcaagaaag aggagctcgg
      541 tgtgatggct ctgcgcggcg gcgatgtggt cggtgaccat acggcgtatt tcctgggcca
      601 gggcgaacgc atcgagatca aacaccaggc gacgaaccgc gacacatttg ccgcgggtgc
      661 gctcgccgca ctgaaatttc tgcagggcaa aaagcccggc ctctactcga tgaaagacgt
      721 gctcggcctt ggctgatgcg gcaccttcgc tggctgcttc tgctgctttc atcggcgctt
      781 gcgtgtgctg tatcttgcag tggtggcaaa ggcgcccgcg aactcaaagc cgttggcggt
      841 gtgctcgatc tctcgcagca tgattttcac agcgagcctg tcattacact gggcggcgag
      901 tggctgtatt atcctgatca actgctcacc agcgcagctg aggcgaagac tgtccaagga
      961 gtgcctgtca tcataccaca gacctggtcg ggctatggct ttggtaccta ccatttgcgc
     1021 ctgaccctgc ctgatgcaca cgaaatcttt aagctgcaca tggaagcaca gctttccgct
     1081 tacgatgtct atatttccgg agtacggcga gcggcctcgg gtttgcccgg caagaccatg
     1141 gctgagacga agcctgcgac cgaaccactc agcgcggtgc tcggcgctgc cggcgaatac
     1201 gatatcttcc tgcgcgtcgc gaactttcac catcgcaagg ggggcgtctt caagaagata
     1261 caggcgggaa gggccgcggc gctcgatcac cttgtttacc agaaacgctt cttagacatc
     1321 ctgctggttg gcgttctgct gatgagtggc atctattatt ttatcgtttt tcttttccga
     1381 ccgcgcaaca aggcggagct ttttttcgcg gcagtctgct tcatgatcat gggccgcacg
     1441 gtcaccacgg gtgaaaagat actgttgttg tcctggcctg aggcaccttt tgccttctat
     1501 accatggtgg agtatctttc gtatttctgg ggtataccgt ttgcactaag tttcttctct
     1561 gcgctgtacg ggcgccttgt gcaccgtttc ctgctgcgag gcttttatat ctcttctgtt
     1621 gtcttctcat tgcccgttgt cttgctgccg ttgcgactct acagccacac agcgtatctg
     1681 tatattcctg tactcgtcgc aggattgatt gtcgtcgtgc ttattcttgg ccgtgcgctg
     1741 ctgcagggat tacctagcgc gggtcttttg gccggtggat tctccgtgct tgcgctggcc
     1801 gtgatgaatg atgcctttaa tacactcgaa ctgctgcgca ccggttatct ggtgcaatac
     1861 ggtttcatgg cgattgtcgt gtgttattcg gctgtgctcg cggcgcgttt ttcgcaggcg
     1921 ctcgaatcga gcgaacgcgc cgagcaggcg ctgcgcgagg cgaatgaaac gctcgaggcg
     1981 cgcgtcaaag aaagaacaca ggagcttgaa tcggcaaaag atgtcgccga agaggctaac
     2041 cgacagaaag acaactttct cgcggtcgtc tcgcatgatt tacgctcacc gctctcggga
     2101 attctgagtg cgctgcaggc gatggggttg tctgcctccg ggggtgagct ggctgctgcg
     2161 gcagaaaaga gcgcggtgcg tatgctcaag actatcgata gcctgctcgc ggtccggcgc
     2221 atcggcaatg ccccgcgccc ggtagatgct gaggttttac ctgggcgcat cgccggtgaa
     2281 attctctctg aaatggaggc agaagcagca aagaaaaaaa tccgcctgct gaatgagtta
     2341 ccgacgcact gggcaatacc gtcaaacccg gaagtcctgc gccacgtgct gcgcaacctg
     2401 ctttccaatg ccgtaaaatt tacacctgcg ggtggctgcg tgctcatcta ccgccccgat
     2461 ggtgacaggg ccgcgattac ggtccgtgac gagggtattg gcatcggtcc tgaagatatt
     2521 gctgccattc tgggcagtaa aaagattact ccccggccgg gtgtctcggg tgaaaagagc
     2581 agtgggctgg gtcttagcct ctgccgggag ctactggcgc gcgaaggtgc caggctggaa
     2641 attgtctctg aacccggtaa gggcagcgct tttaccattg tctggtccga ttagcaggtt
     2701 ccggaacctg cttctggctc ctttttcagg agcctgttaa acgatttaca cgccttcgcg
     2761 ggctacgtgt tcaacctgaa agggcggcgc agccctgtaa tcttacccaa gtcagtgtaa
     2821 ctatgtcaga atttcatatc gaaggcggcc acaagattgg cggtaccata accgctcagg
     2881 gcaacaagaa tgaggccttg cccgtgctgt gcgcggcgct catgaacccg cagggtgtta
     2941 tcattcgcaa tgtgccgcag atagaagata ttaagaatct gctcaagatt atcgaggcgg
     3001 tcggcgcgaa tatcaggacc ctcgaagacg gtcactcgta tgaaatcacc accgccgaaa
     3061 tcaagatgag cgagctgccc accgagctgg ttgccgggtt gcgcggctcg attacgctgc
     3121 tgggtccgct gctggctcgg gcgaaaaaag ttttcttacc ccggcccggc ggcgacaaga
     3181 tcggtcgtcg ccgagtcgac acgcacctgc tcgcgctctc ggcgctgggg gcgaaggtcg
     3241 aggtcttcaa cgacggctat atgctcgagg cagaaaaact caagggtaca gcaatacttc
     3301 tcgatgaagc ttcggtcaca ggtacggaaa atgccgtcat ggccgcagcc tgcgccgaag
     3361 gtgtgacgct cattgaaaac gcggcctccg agccgcacgt gcagggtctc tgccgctttc
     3421 tcgtcgcgca gggtgtgaag atcgaaggta tcggcagcaa catgctgaag attcacggcg
     3481 tggggggatt tgaaaacctt aaagctgccg accacaccat cgggccagac tacctcgaaa
     3541 tcggttcttt tgtagcgatg tcggccatga ccggcggcga tctcgtcgtg caggatgtta
     3601 acccgatcga cttgcgcatg atcaagttcg tttttgagcg cgtcggtatc aactggcgtt
     3661 accatgagag agagggtaaa ggcgctgcag gtaacaatca cattgtcgat ctcgtgatga
     3721 agtcccctta tgcgctcaag atacagaccg acgtgcacgg cgaaattccc aagattgacg
     3781 atgcgccgtg gccaatgttt cctgcggacc tcatttcgat tattctcact gttgcgaccc
     3841 agtgcacggg cacggtactg atacacgaaa aactttttga aagccggctc tactttacag
     3901 acaagctgat gggcatggga gcgcagattg tcctgtgtga cccgcaccgt gcagtcatta
     3961 ttggtccctc gaaactttac ggtagtcgca tgacatcgcc tgacatccgc gccggtatgg
     4021 cgctcgtgat tgcggcgctc gcggcagagg gtacatcggt cattcagaat atcgtgcagg
     4081 tcgaccgtgg ttatgagaat atcgatgcac gactcaaaac gctgggggca cgaattgaac
     4141 gcgtttaata gggtcagccg tgcatgccgg ctgaccagaa gagggatttg tgcagctctt
     4201 tgacgaagaa gccgaacagg ttgtgctcgg ctctgtcatg cagacgccgt cgctgttcga
     4261 agaggtcgcg caggtactga aacccgaaga cttctactac gaaggccacc gcatcatcta
     4321 tgaagcgatt gcttctgaaa ttgaccgcgg tgagcagcca aatgctatcc tgatcattaa
     4381 cgcgctcaaa aaccagaaca tgctcgaacg cgccggcaac cgtgcgggca tcatgaacat
     4441 cgcttcgctg ggtgtgctcg tcggcataaa cacctttgcc gagaaaattc gtgaactttc
     4501 gctgcgccgc cggctgatca tcagctttcg cgagctcgaa aaaaaagcgg gcgatacggg
     4561 ttttaaactc gataccgtac ttacagatac cgaaggcgcc ctgacgcatc ttgccgaccg
     4621 ctacagcagc tataatgtcc gccatattaa ggataccaac aaagagtttc tcgagtttct
     4681 caagcacgtg cgtgaatcgc gcgatggtat caccggcacg gcgacaggat tcaagaaact
     4741 cgatgagctc accggcggtt tcaagccagg gcagatgatt gttatcgcgg cgcgccccgg
     4801 tatgggaaag accaccttcg cgctcaatat tgccctgaat gtctgcatga acaaggcacg
     4861 tccgatggcc gtggtgtttt tctcgctcga gatgacgcgc cttgaacttc ttctgcggct
     4921 tctctctgcg cgcgcctttc tggattcaca gaaggtgcag aaggggcgta tcaatgacaa
     4981 agaaatggca cgactggcgc acacggcgaa agaaatctat gagtcagaat tctacatcga
     5041 cgacagcgct gacctgacaa cctgggagtt caagcagcgt gcccgccgcc tcggccggac
     5101 aatcaagcag cagggcaaag agctcgggct gatcgttgtg gactacctgc agctgatgac
     5161 cgaccgcgac cgcgcgcagg acgggcgtca aaacgaggtt gcggcgattt cgcgcgcaat
     5221 caagctgatt gccaaagacc ttggtgtacc gattctcgca ctttcacaga tgaaccggtc
     5281 gattgagcag cgcggcaaag atccgcggcc gcagctttca gacttgcgcg aatcgggggc
     5341 gatcgaacag gatgccgaca tggttgtctt tatccaccgt gagcagggta aggatattcc
     5401 cgaagatatg aagaacattg ccgagatcat catcggtaag aaccgtggcg gtccacgcga
     5461 cgatgtgaag ctcgcgttcc tgtccgataa gagccttttt accgattacg aacctgcggc
     5521 gcctgcagaa tagtctcagc aggagcctgt tagaacaacc agagctgctc gccgggtttg
     5581 gctgtgtaga ggttagagag ctgtattcca agcagccgaa tcttttcttt ggccagcttc
     5641 tcttcgctga cattttgcgt gaacaggcgc caggcagttt caaagagcgt ctggctgccg
     5701 cgtgccggta cgggaattgt ctgtgagcgc gttatggtgt gaaagtccgc aaatctgacc
     5761 ttgatagtaa tggtgcgccc agaggtattc ttgctggcaa ggcgacgcgc caggtcgtcc
     5821 gcaatttcag taaatgcggc ctgcacctgg ctaaggttct cgaggtcggc ctggttggtg
     5881 cgctcttcgc caaacgattt gcgttcgtgc gtggtttcta cctcgtcatc gtcgatgccg
     5941 ttagctattg cgagtaagcg ctgcgcccat ttgccgagtt cagcacccag ttgatcggcc
     6001 tgtgccgtct gcaggtcacc gatcttgcgc agtcccaacc ggttgagtgt ttctttagcg
     6061 cgtggtccga caccgggtat cgcttcgaca ggcagtggag caagaaactc ccgctcttta
     6121 cccgggtgga caagggttaa accgtcgggc tttttgatct cagtggccat cttcgcaaga
     6181 gttttgcccg acgcgatgcc gatagtgcag gtcaggccaa ttttgctgcg gatggcttct
     6241 ttgatctttg cgcccaggtt cttccacgtg ccgtattttg ccccggtttc cgagcaatcg
     6301 agtacggctt cgtcaattga catgcgcagc aggcggtcgc tgaactcggc cagtacctcc
     6361 atgaccgcgt cagagactgc gcggtagcgt tcgaagcggt gcggcatgaa gattgcctct
     6421 gggcagagct tgtaagcggt gctcactggc tgcgcagagc gaactccgaa tttgcgcgcc
     6481 tcgtagctcg cagcgctaac gacgcccctg aggtgaccgg ctttcgggtc agcaccgacg
     6541 acaacgggtt tgccacgcaa ctctggatga tcgagcacct cgacgctggt gaagaaagcg
     6601 tccatgtcga cgtgaacgat ggtgcgaaac cctgtgctca tcggcctgct accacatgtt
     6661 tacgggcacg ctggcgataa atccaataaa ggtttacact gtgcgcacgg ttcaaagacg
     6721 aaagtcatga aacgcggtat ctttctcagc ttttttgcgt gcctgctgat catcccggcc
     6781 tgtagtagta cgccgaagcc aatccctctt gtgcagacac gcgcactggc tgccgccaac
     6841 cccggtctcg gtgcgctcga gcagcagatt gcgaccaaaa aggccgagca ggatgtgctg
     6901 gaacagcggc agtctgagct gaccgaaaag agcgcgcagt cgcccaaaga tgaaaacctg
     6961 aagcggcagc tcgaccgcct tgaaaaagaa aacgcgctct gccaggcaga acttgccgca
     7021 ctgattggcc gccaccagct gcaatcagcg cgtatcctgc agcgcagcga cattgctgca
     7081 catgaaaagg cagtcgcgaa actcgatgag gcagtggccg atcgccgtaa cgccctgcag
     7141 ggaggtaaat aatgcgctgg ctctctattg ttctgatcgc tgctatcgct cccctttacg
     7201 ctgatctttt ctattaccct tcggaatata gcaagctaca gaacgatatt tacgcgctgg
     7261 gtattcagaa gagtattctt gaggcccagc tggctaacga aaagaaagac gcggcattcc
     7321 agaagaaccg ttttgaagaa aagatccgca atctcgaaag ccgcatcgcc agccttgaag
     7381 ataagttgcg cgcgacagag aatgcactgc gcgacgaacg gcgcggtcgc gttgaagaca
     7441 atgcctacag ttcgctgagg ctcgacgaaa aagaccggca gatcagggcg ctgaggcgcg
     7501 aaagcagcga cactcaggaa aaaatggctg ccgatgccgc ggcgcaggaa aagaaatacc
     7561 acgctgaaat ccagaagctc aaggcggagc ttgaacagaa ccgcgaagaa ttccgcaagc
     7621 agctggcaga actcacagaa cagaaaaata agcgcattgc ggagctcgaa gcagccttga
     7681 gccaaaagtc gcgctccctc gccgaatgcc ttgatcttgc gcagcagcag aaacagacag
     7741 tttcagaact ggaaacccag gcgaaagaac tcgaagacaa gctgcagaaa gaaattgccg
     7801 aaggcaacct gcgtatcaag cgctttaaag atcgcatcgt catcaatatc gacgacaaga
     7861 tactttttcc ttcagggtcg gcgcaactgc ggcgcgaagt caagaagacc ctcgacacgg
     7921 tagcggacat gctggcgcgc aacaagggtt cgcgcgtgct gatcgaggga cacaccgaca
     7981 atgtgccgat caagaccccg cgctttcccg acaactggga gctttcttcg gcgcgtgcac
     8041 tttcggtgct gcgccacctg ctcgaaacca agcacctcga acccgcgcgt tttggcgcag
     8101 ctggttacgg agaataccgt cctgtcgcgc cgaacgatac ggcagcaaac cggcagctca
     8161 accgccgcgt cgatatcgtg ttgcagctgc tcgaagcgca ggggggtgac aagtgagttg
     8221 gcggggagtt atcgccctga cgagtctctt tctcagcacg ggttgcggcg atcgcccagg
     8281 caaagcagct agctcgctgc acctggctgt tgacgccgaa attgcgcagc tgttgcccgg
     8341 actcgaggcc ctgcagttcg aaggccctgc cgttggcgag acgctggcga aactgaacag
     8401 cagctacgaa gagtggtgtg ccgcacccgg caaggccacc gggctcatgc gcgacctgac
     8461 cgaacaggaa cgcaaggcgc tcaaagccga cgactttgct ctgcgcaccc ggctgggttg
     8521 cagcgcctgg tatgcgctga ccgaccagtc ggccgagcgt gactttgcct atacgcgcaa
     8581 ccagatcgac gccggtctca ggcaaagtgc gaactgtgtc tccgtcgggt tcatgcagcc
     8641 ctacctgatg gcagtacagc tcgactgcgc gcagctcacc gtggtagacc tgggctttcg
     8701 cactttgtac ctgcatgcag tattgctgcc actcttgacc gcagcagatt ttgccggtat
     8761 cgattctgta ttaacgcaat ttgagaagac gacgggcgtc agcttcaatg ccgtgtgcga
     8821 aagcagtggc gtagcccgtt gcaagatcgc actgatggcc ttcgccgaga aataccgcag
     8881 ccggctcaaa gcgacgctgc tgctttcacc gctggcgaaa tttcgcgcgg aagtcgggtc
     8941 gatgccaacg gtcatttaca tgagcaacgt gcctgacccg cacttcacca gtgcagaaga
     9001 attcaccgaa ttgcaaaaaa atctcgcagg cagcggcgat aagctatacg ccatctacca
     9061 ccaggcggaa agcactaact ttggtgtcta tgagttagca tcagggaaaa tccgtacggt
     9121 atgtgccgat gcctttgttg tctcgcaggc gggccgttac tcgaaagacc gctgcactta
     9181 ttacccgccg agcagacgcg cctttaccac ttactttgat aacgcgtccg aaaacaaggc
     9241 cgagaagaag gtatgccagt tctgacagcc agttaaatct tcgccaggtg aaagttcgga
     9301 cgctctggtt gcccctgcct gcgcacttca aagtgcaggt gtgggccagt agcgcggccc
     9361 gtcatgcctg agagcgcaat tttctgcccc tgcttaactt tggttcccgc cttcacgctg
     9421 tacttgctca ggtgaccgta gagtgtgcgg tagccgttgc ggtgctcgac gatgatagtc
     9481 ttgccataac ctttcttgct gccggcgaat accacagtac cctcggccga agccactaca
     9541 ggtgtgccga tgctacaggc gatatcaatc cccttgtgga acttgtgctt ctcggtgaaa
     9601 ggatccctgc gcacgccgaa acctgaggtg atacgaccat caacggggtg aataaatgcc
     9661 ttgaggttaa agaattcttt ttccgtgggg tcgaagtgca ggccggtgat aaaccagaag
     9721 ttatcctgcc ctggcaccgc gatgagctcg tcgagctcac ggttgaattt tttcgccacg
     9781 ctctggcgtt caccgtaaac cgcaatacct cgcacattcg gcagcagcca ctcgtcgcct
     9841 ttgttgagat cccacagtga ggcaaggcgg ttgacgctcg aaatggtgtc atggttgagt
     9901 acagtgcgcg ccatcacttt aaagaaggta tctttgtctg aaaggcggta gcggcgccac
     9961 ttgatctcgg gtgcctgacc gtcggcgatc gcccgcaggt tctcggtgac ctcgctgcgc
    10021 atctgcttga tttccgggtc gctgtgcgac agcgagcgga tatagacctg gccgcgtgtt
    10081 gccccggccg cgtacgcgga caaaagaaca aaaatcgtca acacgggccg aaacgcgttc
    10141 atggatagat agagggctgg cgaagacagg cctctatcta tccaatcggc atttaccgcg
    10201 ggtcgtttta gcagattatg tcgcataaag ggtgcagaag gggccggcaa ggctttctca
    10261 gtatgcacca ggaccgcact tgagcaatct cccgccgacc ctcatcgaaa ccctgcgcaa
    10321 ggccgaagag tactttgccg caaaaaaaat accctcgccg cggcttgaag cgcagctgct
    10381 ctttgcgcat tttctgaagc tgcgccgcat cgatcttttc acgcagcccg aacgcccgct
    10441 gatcgccgca gagcttgagt cgttgcgcga tgcgatgcgg cgcaaggtcg cgggtcaacc
    10501 gaccgcttat attctgggca gtcgcgattt tttcggccgc tcgtttgttg tcacgccggc
    10561 ggtgctgatt ccgcggccag agaccgaaga gctggtcgaa cacgttcttt cgctgaaacc
    10621 tgccgcgcgg cacgtggtcg atgccggttc gggcagcggc tgcatcggca tcacgctcgc
    10681 cgccgagctc aaggccgccg aactgacgtt gctcgacatt tcttccaaag cgctgcaggt
    10741 cgccgcggcg aatgccgcgc aactcttgag cgaaacagga acgcaggttc ataccctgtg
    10801 tgccgacttt tgtgcggggg agttatccct gtcacaacag gctgagctga tcgtgagtaa
    10861 cccgccgtat gttctgcccg aagagtttgc cgcactcgat gcttcggtgc gcgatcacga
    10921 acccagattg gcgctcgttg cagaaaaatt cgaacagctg caccgcggcc tggttgcctt
    10981 tgcctggcgc aaccttgccc ccggcggtat tctagcgctc gaaacccacc cgcagaagag
    11041 cagcgaggtg gccgaatggg taggtgaagc tggctttgtc agtgtggaaa tccgtaatga
    11101 cttggccgca cgtcctcact ttgtactagc gcagaaatga gctgagctca tttctgcgaa
    11161 aaccactatg aaatcccgcc acatcatcac ctttgtcgtg atactcacct ttctgacctt
    11221 ttactttatt ttcgataacg tcttctcgat ctgggaacag gcgtcgtaca cgcgctttcg
    11281 cccggtgacg acgggcctgc catcggcgca gatttttcgc gacgtgcagg tcggcgatgc
    11341 caccgcagat tttttcgtcg ccccggccgg cagcgcgcgg cggcccctgc ttgtcgtgat
    11401 gcacggcggt ttcttgcagg gcggcaacaa gaagaactac gcgtacctgg gtggtcttgg
    11461 tgtgcgccag ggttttgtcg tcgccgtatt gcagctgccg aattatccgg gtattctcag
    11521 taggccgttt ttttctgctg agacactgca tgcacgtgct ttacccgagc agtcgcgacg
    11581 ttttgcccgt ttcgtgacca atgtcgcagc gctcgccgat cgttttaact tcgacgcgaa
    11641 gaagctgcac gtgatggcgc atgcctcggg tgccctgctg atcggcgaag cagatctatc
    11701 acagttcaag agcgtgacgc tggtgtcgcc gattctttca ttgaaggaca atgtagcgaa
    11761 gattgccccc atgcagctac gcgcggtcga tggttatatc aaggatgccg acgcgcttaa
    11821 actctcgccc gccgagtggt tgccccggac taaaatgccc gtattcctgc tctgcaccga
    11881 gcgcgatttg ccttacatca aagatgcctg caaagaatta ccgctcgcgc gccccggcgc
    11941 ggctgctgtt gaacgcgtgc tggtcgagaa accctcgcac ttcgaactga tgttccacct
    12001 gggcagcaag atcgaagaag cgaccgaccc gctgaaaaaa ttctggttta ttaattcccg
    12061 tttgtgaacc gccttcggcg gttcacaaac ggaatgcctc aggcagaatt ttgtcgatca
    12121 ggatcttctt catgcccttt ttcccgtcaa agaaaacaat cccgcccttg ccgaattcaa
    12181 atatcacctg gcggcatgcc ccgcacgggg gaatttcggg cagcttgttg ttcaccacgt
    12241 agatcgcgcg tatcagtctt tcgcgctcgt cgctgatcgc ctgcatcgcc gccgaaaccg
    12301 ccgagcgctc ggcgcagatc gtcaggccat acgaggcgtt ttcgacattg caaccgagat
    12361 agatgttgcc gtcgttcgcg agcagcgccg cccctacctg tatttgcgag tagggggcat
    12421 acgcctgccg cgaagctttg actgcctgct cgcgcaggtg cgtgatggtt tgtttggtta
    12481 atgctttcat tttcaggaga tgtttgtggc agattgaaac tccccttcgg cctgccgtgg
    12541 tcaactcttt gccatgtgcc cggccaggta ttgcctttgc cgatcgtgga caaacacttg
    12601 acgctccgga ttattatgac taaaatagtc atatgtacca gtctactata tcccactcaa
    12661 aagcggtgtt cagctcgctc attgaccagg tcaaagccgg tgaaactgtt atgatcaccg
    12721 accgcgacgt gcctgtcgcc ctactgacgc ccataccgcg cgagaactac aaggggtctg
    12781 agctgctggc cgaccttgaa cgccagggcc tgctgcgccg agcaaccgct gcaccctgtg
    12841 ctgtgctcga tgatctgccg ccgaagaacc agctcactgg tgcgctggct gccctgctcg
    12901 aagagcgtcg cgaagggcga tgattttctg ggatagctcg gcggtcgtgc cactcattgt
    12961 gcgcgaagcg agctctgaaa aactgcttca aatctaccag tcagaccccg aaatgctcgt
    13021 ttggtgggcg acggagcttg aatgtgtctc tgccatttgc cgccgcgagc gtgatgccaa
    13081 tgtacacgct ggtcacgcca gttcggcatt tggccgcttg aaacgactgg ctgaaacctg
    13141 gcacgaggtg caggcaggcg accgtgtcaa agcgaccgcg cggcggttac tcagaacgca
    13201 taacttgcgc acggctgacg cgctgcaact tgcggcagcg gttacgatat cccagggcga
    13261 ctcctcgcgc attacgtttc tgacgctcga cgaccgcctg cgtgttgctg cggagagaga
    13321 ggggttggcg
//
LOCUS       DAOQJF010000043        13249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2290_length_13249_cov_5.953388, whole genome shotgun sequence.
ACCESSION   DAOQJF010000043 DAOQJF010000000
VERSION     DAOQJF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_13249_cov_5.953388"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLF85_03785"
     CDS             <1..1009
                     /locus_tag="PLF85_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18809.1"
                     /translation="EAIPTELYWRKSFENVSISAGKIITVWGKADILPVSDVISARDA
                     TKAFFAQPQEARLGQNTVRVDWFLPRQEIQLLFIPYPVNTRYTDRDHPYALLPGVEIH
                     KASAYGREVEGGGQYTAYLGGGAISVFGGRFYNRDPLFRLLPDYSFEAVHAPYATTGV
                     SANFPLWGMLLKFDSAYQFKRLSQTATQLPNPQCPQTCGTITMPYDAKGVDQVGFTVG
                     LDYNTTEYGSFILEYSVAGPTRKDPQLLRDDPLHSYAFGYSKNFVRDTLSFNSFVVTF
                     LKYSNILFRFNVTYKLTDVISAYVQYTGVFIASGDPDLRIFDKYDRFDLQLSYQFDLA
                     K"
     gene            1031..2434
                     /locus_tag="PLF85_03790"
     CDS             1031..2434
                     /locus_tag="PLF85_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNE18810.1"
                     /translation="MSEVLHHPEPAPAFPARRFDRWKMVLSIAWPLIVANSFWNLQMT
                     IDRLFLAEFSTEAMGAAVAVMGVFWTPMALLQQTASYVTTFVAQYFGAGRKDMIGPAV
                     WQSLWVSALGGFLFLLLIPATTAIFSAMGHAPSMQQLEGDYFVAMCFSALPTAIVAAT
                     SGYFTGLGRTQVIMQINGVGLVANAVLDYFMILGKGGFPEMGIAGAGYATAIGTALAA
                     LYGLWLVFSDRERREYGMNSGWRWNRELMARFIRFGVPSGMQWALEGLAFSIFLIFIG
                     RMVDGNAALSASSIVVTVMMLSILPALGMAQAAAVLVGQYLGEKRPADAEAAVWSGWQ
                     VAVLYIMAVGLTFVLVPGFYLGWFENKKDPELWQKVMQMAPYLLIFVAIFTTADSINL
                     IFSFALKGAGDTRFVTIVAFLLPWPLMVLPTYLLKDQPGAVYLAWGAGTVFSLTQATI
                     FWRRFVGGKWKRMSVIG"
     gene            2673..3770
                     /locus_tag="PLF85_03795"
     CDS             2673..3770
                     /locus_tag="PLF85_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNE18811.1"
                     /translation="MLAAEDEGRTEEPTELRRRREREKGRVPKSAEIPAALGTLGGLI
                     VLFASAGLILSSLARIIKLYIGNFNSLGTVQEATMIPMMLAVAREFFLMLAPFFAAVM
                     VMGIIGNLTQTGFLFSLEPLRPKLSNISFSFNRMVERIFFSKQIYVNLAKTIIKIVLI
                     GAASYMIISDDFLALMKTGNMGVGEALRSIGYMAFKLALVLIIILLAMSVPDYLFQRS
                     QFIESLKMTKQEIKQEYREQEGDPQIKARQRQRMQEALRSSMRQAVATADLVITNPTH
                     YAVAIKFAFDEEGTPPRLVAKGEDEIARIIREVAKRNNVPMYEDKPMARFLYDNVELN
                     QLIPGQLLELLARIVAAIPSLSAKFQRDRVA"
     gene            3770..5854
                     /locus_tag="PLF85_03800"
     CDS             3770..5854
                     /locus_tag="PLF85_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNE18812.1"
                     /translation="MKKWYQQGDIMLGIAVIAVVAMLIIPLPAMLLDFLLAVSIMISI
                     VALLTAMFNREIADFSVFPTLLLVSTVYRLALNVSSTRLILAKGPAFEGKLIRAFGEF
                     VVGNNYVIGFVVFIILVLVQMMVITKGATRISEVSARFALDALPGRQMAIEADITSGR
                     VTEEEGRRRNENLRREVDFYGQMDGATKFVQGDVRVGLIITAINIIGGLIIGMAMRGE
                     NLATALKTYTLLTIGDGLVAQIPSLLITTATGMVVTRAGAKNDLTTELSVQMFQNSRA
                     LYITAGALFAASLVPGFPKISLWVISLFLGFLAYNMRQQKKAESQKQEAIQEQQKVGS
                     TERFLDELAVDPLKIEIGFNLVALLDRKQGGTLLDRITNLRQKMASDLGMIVPPVRIN
                     DNLDLDGNEYVILIGGIEVARTKVYPEYLGALSNPNVREEIKAVSPELVVTDPAFGQR
                     VILIDPEKKREAEEKGYIVIDASTMIITHLQETFRAYAPQIMGREEVKLLLDKTRQKY
                     PSVVDEVGKLFPNPGVLLTILHNLLRENVSIRNLPAILETLADYGQRVQNPELLTDIV
                     RQRLARQIVAPYQDKNKALFAAVIDPQIEYDVRGNITIDERDGRVLALDTHYRERLRA
                     ALLNAYNAMQNGGKFPVFVCGSEIRSAIAEIISREISTRSFAVIAYEEIPRDIRFVEL
                     TKVIMEESEVNA"
     gene            5854..7131
                     /gene="flhF"
                     /locus_tag="PLF85_03805"
     CDS             5854..7131
                     /gene="flhF"
                     /locus_tag="PLF85_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNE18813.1"
                     /translation="MHYFTEHGDSHAEVLAKVRSKYGSGPEVLISHQREVPVKSVWGK
                     ITRATRWEISGAILEKKQVQPKKKDETIDNKLKLLEEMLNRTSFRKPAEGQVQGVSAD
                     RAPLVRELEPNLRKEILPIGVVPPSAEQIRRLEAEVAELRQALKSKGGPDLDLVKERE
                     LLSLYEHCVAIGFSPAFAESLAMRTRAEVASTDYKLRKKVHAKAREVLAEHIRTTTSA
                     SQRIVTLVGPTGAGKTTTLVKLAARLSVMQKRRVELITIDNYRIAATEQLKVYARIMD
                     IPCKVCRTPDELRTAIDASEAEFILVDTSGSSPANRDFLARQKTFFDAVHAQHAIETH
                     LVIPATTRLQDAKLLFDRFELFNYSRVIISKIDESYSFAPIVEMADTWHRPFSMLTNG
                     QDVAKDWLDADRLLIADALLKKWLDESESIDRK"
     gene            7199..7999
                     /locus_tag="PLF85_03810"
                     /pseudo
     CDS             7199..7999
                     /locus_tag="PLF85_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
     gene            8002..8505
                     /locus_tag="PLF85_03815"
     CDS             8002..8505
                     /locus_tag="PLF85_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18814.1"
                     /translation="MPSLFTAAHLKLSLLLSVLGALVALVFTVISDSGVVAIIFRPLV
                     SGLLMFLLGTALYTLIAKKVPEAIEAIEAPPMDIADMPPSEDFPEGETGGSELALEND
                     EVSGTGGGMDAYNSDGGPASAGSSIPRKTGVQIGKDEITVNGVKFKNQPEVMAETIKQ
                     LMDQDDA"
     gene            complement(8527..9387)
                     /locus_tag="PLF85_03820"
     CDS             complement(8527..9387)
                     /locus_tag="PLF85_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE18815.1"
                     /translation="MRLRNELLLVFAMVIWSGSWTSGKLIAGSADPNTTLFWRFIVSA
                     LALAPMVRFAGESFRISARQATWAALGGLIITVYTFLFFIGLKTGYAGAAGVLVTSMN
                     PIFTYFLTRIFQGQNLNRRDGIGLMLGVSAGVILLELWSVSGEKLLLSGNLLFLVCAM
                     IYALVTMTTNTATRSMSILVYSFYVNLLGALFVLPLALVHWQQTPVFSDTTYWFNIFY
                     LGVISNAFATTVYFYAVKKLGSARASSYIFIVPATALLIAALYLDEPVRATTLVGGLL
                     ALVGVTLLRR"
     gene            complement(9435..10937)
                     /locus_tag="PLF85_03825"
     CDS             complement(9435..10937)
                     /locus_tag="PLF85_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18816.1"
                     /translation="MERTFLQHLRYGGIALLLLTTLHCAARQVRSEIELEEAARRAAA
                     NEEKGWQALERTIRPKDEDPDVEMQKKALREVGKIPSPRSEVLLTENLTNRYMRAEAA
                     EGLMQQRNDSNRAQIDQAIINAARSNAQEFSGLTREEIRALGESDNPEAVRLLKQQIG
                     RDPNKDEIVVESLGKILRRQKRSSYSPLDFTLPLGQAPATGAQLQFEDISANKDEAPP
                     AAATAADNSEDTDPEKVLLEFLGTDSVPETKDKAVQSIADAHGGKVDYLLGLAAKSSL
                     PAATRIAIIDYLTRYAVNNQDKSMIGRFMALKRRAASREVTASIDLSIRILGSAFGTK
                     VATGGRTRRVVITDGYDPLPKEADIITLKQKPYPEYSAADVKTNLKKALRHYRMDAAL
                     AERMQKRVNELLNLPENRQSSERNLIFAALGRLYPQKDFYVLKEQGQDAFGKPGYFTT
                     TLRLVTSSARGRSWQIAALQRVWGLTYAEADILRQIYLRDGKLLQQRMRL"
     gene            11124..11435
                     /gene="rplU"
                     /locus_tag="PLF85_03830"
     CDS             11124..11435
                     /gene="rplU"
                     /locus_tag="PLF85_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNE18817.1"
                     /translation="MQAVIQVGSKQYKVAQDTVFFAELTGTEPGKDLTIDSVLSVNNN
                     GQVKIGQPFVSGASVTAKVLEEVKGPKIVGVFYRTKKHNHRKWGHRQKYHKLQVTSIK
                     A"
     gene            11547..>13249
                     /locus_tag="PLF85_03835"
     CDS             11547..>13249
                     /locus_tag="PLF85_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNE18818.1"
                     /translation="MKFVHYLKRFYEIFIRGKITLYLLLIVAYFGVRTFLAYNRWNDE
                     EPAILQKLTLYKQQLDRLRSGAIAEDTVASIEPGAVAIPSKVFDRHNKLVGEFYTERR
                     SLLNYEHFPPFVTDALMASEDRRFYEHHGIDYAGIMRAVIRNLLHFRFAQGGSTLTQQ
                     LAKVLFTNQEKTLDRKLFEYFCARAIEERYSKKEILEMYFNLIYMGHGIYGLETASQF
                     YFNKSARALTVGEAAALVGVIPNPKAYSPMGDLPRFLERQLAVMRSMVAIGKLTEDKL
                     KNERRRFFQAWRIAAKDSKQSGIGDFPDRMTGKNYAPFFLDYLRQKLTAQFSQDALTR
                     SGLRIYTTLDLGRQIAAQKAVKAAVELQKQHYEKLLAGARKKNNAAKIQEYEKALATT
                     NAAFISIEPKTGYILTMVGGGEFSSQNQFNRALKAERQIGSTIKPLIYYLALQRQILT
                     PASLIADEPLQDYQNYDGKFLGPITLLDALKLSRNPTAIRALQKLDFDDLRSLFHEVL
                     QLPYSDLEKKIPRELGVALGSSSFTPLQLAQMYATMLNQGRRVEPRDLLRIEDSQGRQ
                     I"
BASE COUNT         2921 a         3666 c         3694 g         2968 t
ORIGIN      
        1 cgaggctatt cccactgagc tctactggcg caagtcgttc gagaatgtga gcatctcggc
       61 tggaaaaata attacggttt ggggtaaggc cgatatcttg ccggtatctg acgtcatctc
      121 ggcgcgtgat gcgaccaagg catttttcgc gcagccgcag gaagcgcgcc tcgggcagaa
      181 caccgtgcgc gtcgactggt tcttgccgag gcaggaaatc cagctgctct ttattcctta
      241 tccggtgaac acgcgctata ccgaccgcga tcatccttac gcgctcttac ccggtgttga
      301 aatacacaag gcctctgcct atggccgcga ggttgaaggc ggtggccagt acacagcgta
      361 tttgggcggc ggagcgatct ctgttttcgg gggcaggttc tataaccgcg atccgttgtt
      421 tcgtctgctt cctgattaca gctttgaggc ggttcatgcc ccttatgcaa ctaccggtgt
      481 ctctgccaac tttccgctgt ggggcatgct gctcaagttc gatagcgctt accagttcaa
      541 gcggctcagc cagacagcga cacagctgcc gaacccgcaa tgtcctcaaa cctgcggcac
      601 gatcacgatg ccgtacgatg cgaaaggggt cgaccaggtc ggtttcacag tcggactcga
      661 ctacaacacg accgaatacg gcagtttcat tcttgaatac tcagtcgcag gcccgacaag
      721 aaaagatccg cagctcttgc gcgacgaccc gctgcattcg tatgcgtttg gttattcgaa
      781 gaacttcgtg cgcgatacgc tcagcttcaa ctcgtttgtt gtgacgttcc tcaagtacag
      841 caacatactc tttcgcttca atgtaaccta caagcttact gatgttattt ctgcttatgt
      901 gcagtatacg ggcgtcttca tcgcttcggg cgatcccgac ctgcgtatct tcgataaata
      961 tgaccgcttc gacctgcagt tatcgtatca atttgatctg gcaaaataga ccataaaaaa
     1021 aggaaatcca atgtctgaag ttcttcacca ccccgagccc gcacccgcct ttccagcgcg
     1081 gcgttttgat cgctggaaaa tggtgctttc gattgcctgg ccgctgattg tcgccaatag
     1141 cttctggaac ctgcagatga ccatcgaccg gctgtttctt gccgaattct cgacagaggc
     1201 aatgggtgca gcggttgccg tcatgggcgt cttctggact ccgatggcgc tgctgcagca
     1261 gacagcttcg tatgtcacaa cttttgtcgc gcagtatttc ggagcgggca ggaaagatat
     1321 gatagggcct gcggtctggc agtccctgtg ggtcagcgcc ctgggtggat tcctgtttct
     1381 gctgttgata cccgcgacaa cggcgatatt ttctgcgatg gggcatgcgc cgtcgatgca
     1441 gcagcttgag ggcgattatt ttgtggcgat gtgtttttcc gcattaccca ctgcaatcgt
     1501 cgcagcgacg agcggctatt tcacaggcct cggtcgcaca caggtgatca tgcagattaa
     1561 cggtgtggga ctcgtagcca acgcagtgct cgactatttc atgatcctgg gtaaaggggg
     1621 atttccggag atgggtatcg ccggcgctgg ttacgccact gccattggca cagccttggc
     1681 ggcgctctat ggtctttggc tggtgttcag cgaccgtgag cgccgtgagt acggcatgaa
     1741 ctccggctgg cgctggaatc gtgagctgat ggcgcgcttc atcaggtttg gcgtaccttc
     1801 gggtatgcag tgggcactgg aaggactcgc attcagcatc ttcctgattt tcatcggtcg
     1861 aatggtcgac ggcaatgctg cgctttctgc gtcttcgatt gtcgtgactg taatgatgct
     1921 ttcgattttg ccagccctcg gcatggccca ggccgcagcg gtactggtgg gtcagtacct
     1981 cggcgagaag cggccggctg acgccgaggc tgcggtttgg tcgggctggc aggttgcggt
     2041 actctacatc atggcagtgg gattaacctt cgttctggtt ccgggtttct atctcggctg
     2101 gttcgagaac aaaaaagacc ccgagctttg gcagaaggtg atgcagatgg ccccgtacct
     2161 gctgattttc gtcgccatct tcacaactgc ggacagcatt aacctgatct tctcgttcgc
     2221 gctcaagggg gccggcgata cacggtttgt caccattgtg gcatttctgt tgccctggcc
     2281 ccttatggtt ctgcccactt acctgctcaa agaccagcca ggcgctgttt accttgcctg
     2341 gggggcgggt accgtcttca gcctgacgca ggcaacgatc ttctggcggc gctttgtcgg
     2401 cggcaaatgg aagcgcatgt cagtgatagg ctgaccatac accactgcgg gccgctccgc
     2461 ggcgtgcagt ggtgcggcgg ctaaatcctt gacaggtcag ctatcccgta ttatgtcgca
     2521 ttattgcggc gtggatttca tcctttattg cgctctggcg ctcatttgtc tgccggctaa
     2581 attccggtct cgaccgccta ttaccccagc ctgaccttct tgttcccgcg tttgctccgg
     2641 gcgccggtta ctcggatctc gattcgctcg tcatgctggc ggccgaggac gaaggccgca
     2701 ctgaagagcc gaccgagctc aggcgccgcc gcgaacgcga aaagggccgt gtacctaaaa
     2761 gcgctgaaat tccggctgcg ctcggcacac tgggcggact tattgtgctt tttgcaagcg
     2821 cgggcttgat tctctcttcg ctcgcgcgga tcatcaagct ctacatcggc aacttcaaca
     2881 gtctcggcac tgtgcaagaa gcgaccatga taccgatgat gctggctgtc gcgcgggaat
     2941 tcttcctgat gcttgctcca ttcttcgctg cggtcatggt gatgggtatt atcggtaacc
     3001 tgacgcagac aggtttcctg ttttcgctcg agcccctgcg gccgaaacta tcgaatatct
     3061 cgttttcttt taaccgcatg gtagagcgga ttttcttctc caagcagatc tatgtcaacc
     3121 ttgcgaagac aatcatcaag attgttctga ttggcgctgc gagctacatg attatcagcg
     3181 atgactttct tgcactgatg aaaaccggca acatgggcgt cggtgaagcg ctacggtcga
     3241 tcggttacat ggcgttcaag ctggcactcg ttctgattat catattactc gccatgtcgg
     3301 tacccgacta cctcttccag cggtcgcagt tcattgaaag cctgaaaatg accaagcagg
     3361 aaatcaaaca ggaataccgc gaacaggaag gggatcccca gatcaaggcg cgccagcgcc
     3421 agcgcatgca agaagccctg cgttcatcga tgcgccaggc tgtggcaacg gctgacctcg
     3481 tgatcacgaa tcctacacac tacgccgtgg cgattaagtt cgcgtttgac gaagaaggta
     3541 cgccgccacg cctcgttgcg aagggtgaag acgaaattgc ccgcatcatc cgtgaagtcg
     3601 caaagcgcaa caatgttccg atgtacgaag acaagccgat ggcgcgtttt ctctacgata
     3661 atgtcgagct gaaccagctg ataccggggc agctgctgga attactcgca cgcatcgttg
     3721 cggcgatacc aagcctgagc gctaagttcc agagggatcg ggtagcctga tgaaaaagtg
     3781 gtaccaacag ggcgatatca tgcttggcat tgcagtcatt gcagttgtcg cgatgctgat
     3841 tattcccctg cctgcgatgc tgctcgactt cttgctcgcg gtcagtatca tgatctccat
     3901 cgtcgcgctc ctcacggcga tgtttaaccg cgagatagcc gacttctctg tttttcccac
     3961 gctgctgctc gtttcaacgg tttaccggct tgcgctcaac gtttcttcga cgcggcttat
     4021 cctggcgaag ggaccggctt ttgagggcaa gctgatcagg gcgttcggcg agtttgttgt
     4081 cggtaacaac tacgtcattg gttttgtcgt gttcatcatc ctggtactcg tgcagatgat
     4141 ggtcatcacc aagggtgcaa cgcgtatctc tgaagtctct gcgcgcttcg cactcgacgc
     4201 gttgccaggc cgtcagatgg ccattgaagc cgatatcact tcgggccgcg ttaccgaaga
     4261 agaggggcgt cgccgcaatg agaacctgcg ccgtgaagtc gacttctacg gccagatgga
     4321 tggtgcgacc aagttcgtcc agggcgacgt gcgcgtgggc ctgattatca ccgcgatcaa
     4381 catcatcggc ggcctgatta tcggtatggc gatgcggggc gagaaccttg cgacggctct
     4441 gaagacgtat acgctactga ccatcggcga cggcctggtt gcacagatac cttcgctctt
     4501 gattaccacc gcgaccggta tggtcgtgac gcgtgcggga gcgaaaaacg acctgacaac
     4561 tgaactctcg gtgcagatgt tccagaactc gcgtgccctc tatattaccg cgggtgcgct
     4621 ttttgccgcg tcactggtgc ccggttttcc gaaaatttcc ctctgggtga ttagcctctt
     4681 tctcgggttt ctggcttaca atatgcggca gcaaaagaag gcagaatcgc agaagcagga
     4741 agcgatccag gagcagcaga aggttggctc gaccgagcgt tttctcgacg agttggctgt
     4801 cgatccgctt aagattgaaa ttggctttaa cctggtagcg ttgctcgacc gcaagcaggg
     4861 tggcacgctg ctcgaccgca ttaccaacct acgccagaag atggcatctg atctcggcat
     4921 gatcgtgccg cctgtacgta tcaatgataa tctcgatctc gacggtaacg agtatgttat
     4981 actcattggc ggtatcgaag tcgcgcgtac caaggtctac cccgaatacc ttggggcgct
     5041 ctcgaacccc aacgtgcgcg aagagattaa agctgtctcg cctgaactgg ttgtcacgga
     5101 ccctgccttc ggccagcgtg taatactgat cgatcctgag aaaaagcgcg aagctgaaga
     5161 gaaaggttac atcgtcattg atgctagtac gatgatcatc acgcacctgc aagagacctt
     5221 tcgtgcgtat gcgccgcaga tcatgggccg cgaagaagtc aaactgctgc tcgataaaac
     5281 gcggcagaag tacccgagtg tcgtcgacga ggtgggtaag ctttttccga atcctggtgt
     5341 gttgctcact attctgcaca acctgctgcg cgaaaatgtt tctattcgta atcttccggc
     5401 tattctcgaa actcttgccg actacggcca gcgggtacag aatcctgaat tactgacaga
     5461 cattgtccgc cagcgcctcg cgcggcagat cgttgcgccg taccaggaca aaaacaaggc
     5521 cctctttgct gccgtgattg acccgcagat cgagtatgac gtgcgcggca atatcaccat
     5581 cgacgagcgc gatggtcggg tactggcgct tgatacgcac taccgcgagc gcctgcgggc
     5641 tgcgctgctg aacgcttaca atgcgatgca gaatggcggc aagtttccgg tatttgtctg
     5701 tggctcagaa atccgttcgg ctattgccga aattatttcg cgcgaaattt ctacgcgctc
     5761 ttttgctgta atcgcttatg aagaaattcc gcgcgatata cggttcgttg aactgactaa
     5821 agtaattatg gaagaaagtg aggtgaatgc ctgatgcatt actttaccga acacggtgac
     5881 tcgcatgccg aggtgctggc gaaagttcgc agcaaatacg gcagcggacc agaagtgctg
     5941 atctcgcacc agcgcgaagt ccctgtcaaa agtgtgtggg gtaaaattac ccgtgccacg
     6001 cgctgggaaa tcagcggagc aattctggaa aaaaagcagg tccagccgaa aaagaaagac
     6061 gagacgatcg acaacaagct gaagctgctc gaagagatgc tgaaccggac ttcgttccgt
     6121 aagcctgccg aaggacaggt acagggcgtt tctgcggatc gcgcgccgct ggtgcgcgaa
     6181 ctcgagccga acctgcgcaa agaaatcctg cccatcggcg tcgtgccgcc gagcgccgag
     6241 caaatccgtc gcctggaagc agaagtcgcc gagctgcggc aggctctcaa gagcaagggg
     6301 ggacctgatc ttgatctcgt gaaagagcgt gagctgcttt cgctctacga gcattgcgtt
     6361 gccattggtt ttagcccggc attcgctgaa tcgctcgcga tgcgcacacg ggccgaagtc
     6421 gcctcgaccg attataagct gcgtaagaag gtacacgcga aggcgcgcga ggtattggct
     6481 gaacacatac gcacgactac ctctgcctct cagcgtatcg tgaccctggt gggaccgacc
     6541 ggcgccggca aaacgacgac acttgtcaaa ctggccgccc ggctgagcgt catgcagaag
     6601 cgccgggtcg agttgatcac gattgataac taccggatcg cagcaactga gcaactcaag
     6661 gtctatgccc gtatcatgga tattccctgc aaggtctgtc gtacgcctga tgagttgcgt
     6721 acggccatag acgccagcga agcagagttc atcctggtcg acacgagcgg ttcaagccct
     6781 gcaaaccggg attttctcgc gcggcagaag acattttttg acgcggtaca tgcccagcac
     6841 gcaattgaga cgcacctggt gattccggca acgacacgcc tgcaggatgc gaagctgctc
     6901 ttcgaccgtt tcgagctctt taactatagc cgggtgatta tcagtaaaat cgacgaaagc
     6961 tacagttttg ccccgatcgt cgaaatggca gatacctggc accggccgtt ctcgatgctg
     7021 accaacggcc aggatgtagc caaagactgg ctcgatgctg accgcctgct gatcgccgac
     7081 gcactcctga aaaaatggct tgatgaatct gagtcaattg atcgaaaata agaaaagagg
     7141 ctaaatggat caggcaagca cgctcaggca gatggtgggt aagtcagacc ccggtacgac
     7201 tacgcaaatc atcactctga ccagcggaaa agggggggtc ggcaaatcga caatctcggt
     7261 gaacctcggc ctcgcgctgg cctctgccgg caaaaaggta ttgctctttg atgctgatct
     7321 ggggctcgcc aacgtcaacg tgctgctcgg cgtagtgccc aagttcaacc tctaccacgt
     7381 tatcaagggc cataaagagc tcgaagacat cattatccgt acgcctgagg ggctcgatat
     7441 catagcggga gcgtcaggct acagcatgct cgccaacctc tctgacaaag aacgcaacaa
     7501 cctcgtttcg gcgttcgaga agctgtcagg ttacgatatc atgctgatcg acacgggtgc
     7561 aggtgtgtct tccaacgttg tcgggtttac ccttccggcc gacaaggtga tcgttgtgac
     7621 gactcccgaa ccaacggcaa tcacagacgc ttacggaatc atcaaatcga ttgtgctggt
     7681 agcaccggac aagcacatca agctgcttgt taaccgcgcc acctcttcgc tcgaaggcaa
     7741 aaaagtcgct gaacgcgtga tcaacatctc gagccagttt cttaatgtgc gcgtagaaaa
     7801 cctggggttt atctacgacg acgacaatgt tcccaagtct atccgccggc agaaaccgtt
     7861 ctaccagctg ttccagaact gcaaggctgc tgcctgcctg gacatcgtcg cctcgcgcat
     7921 actcgagcag gaattaccca aacgccacga gtctggtgta ggcgcttttt tccgcaagat
     7981 ctttagccca gccgtgtaag aatgccctcc ctttttaccg cagcgcatct gaaactcagt
     8041 ctgctgctgt cggtactggg cgcgctcgtg gcgctcgtct tcaccgttat ttctgacagc
     8101 ggcgtggttg ctatcatctt tcgtccgctg gtgagcggcc tgctgatgtt cctgctcggg
     8161 actgcgttgt atacgctgat cgccaaaaaa gtaccggaag cgatcgaggc aatcgaagcc
     8221 ccgccgatgg atatcgccga tatgccaccg agtgaagact tcccggaagg tgaaacgggt
     8281 ggcagcgagc tggcgctaga gaacgacgaa gtttcaggca ccggcggcgg aatggacgcc
     8341 tacaactctg atggcgggcc ggcttctgcg ggcagctcga ttccgcgcaa gacgggtgtg
     8401 cagattggca aagacgaaat caccgtgaac ggcgtaaagt tcaagaacca gcccgaagtg
     8461 atggcagaaa ccatcaagca gctgatggac caggacgacg cctgattgcc ggctttcctg
     8521 gaatcattat cttcgtaaca gggtaactcc cacaagtgct aaaagcccac ccaccagcgt
     8581 ggttgcccgt accggttcat cgagatagag tgccgcgatg agcagcgccg tcgccggtac
     8641 aataaaaatg tacgaactgg cccgcgctga accgagtttc ttgactgcat agaaatagac
     8701 cgtcgtcgcg aacgcattcg agatcacgcc gaggtagaaa atattgaacc agtaggttgt
     8761 gtcgctgaac accggggtct gctgccagtg cacgagggcc agggggagaa caaacagcgc
     8821 tcccagcagg ttgacgtaga atgaatagac caggatcgac atcgaacgtg tcgccgtgtt
     8881 ggtcgtcata gtcactaacg cgtagatcat cgcgcagacg agaaagagca ggttgcccga
     8941 tagcagcagt ttctcccccg agactgacca cagctcgagt agtatgacgc ccgcgctgac
     9001 gccgagcatc aagccgatac cgtcacgccg attgaggttc tggccctgga agatgcgcgt
     9061 taaaaagtaa gtaaaaatcg ggttcatcga agtgacgagc acgcctgcag cgccggcata
     9121 acccgtcttc agcccaataa aaaaaaggaa cgtatagact gtgatgatga gcccgccgag
     9181 cgctgcccag gtcgcctgcc gtgcgctgat acgaaacgat tcaccggcga agcgtaccat
     9241 gggcgcgagc gccagcgcag acacgataaa ccgccagaag agggtagtgt tggggtcggc
     9301 agaacctgcg atcagcttac cagacgtcca ggagccgctc cagattacca tcgcgaaaac
     9361 caggagcagt tcatttcgca gccgcatctg tgccggtgtg gtttcggctc tcaggccgca
     9421 aaggaagagt cagttcaaag ccgcatgcgc tgctgcagca gcttgccgtc gcgcaggtag
     9481 atctggcgca gaatgtcagc ttcggcgtag gtgaggcccc agacacgctg cagtgccgca
     9541 atctgccacg acctgccgcg cgccgacgag gtcacgagcc gcagcgttgt ggtgaaataa
     9601 cctggcttgc cgaaggcatc ctgtccctgt tctttcagga catagaagtc tttctgcggg
     9661 taaaggcgtc cgagagccgc gaagataagg ttgcgttcac tcgactggcg gttctctggc
     9721 agatttaata gttcgttcac acgcttctgc atacgctcgg cgagggcagc gtccatgcgg
     9781 tagtgtcgca gcgccttctt caggtttgtc ttcacatcgg cagcagaata ttccgggtat
     9841 ggcttctgtt tcagtgtaat gatgtctgcc tctttgggca gcgggtcata gccgtcggta
     9901 atcaccacgc gccgcgttct gccccccgtc gcgaccttcg tgccaaaagc gctgccgaga
     9961 atacgaatcg aaagatcgat cgaagcggtg acctcgcgac tggccgcgcg gcgcttgaga
    10021 gccataaacc tgccaatcat cgacttgtcc tggttattga ccgcgtagcg tgtcaggtaa
    10081 tcgatgatcg cgatgcgggt tgcagcaggt aacgagcttt ttgctgcaag accgagcagg
    10141 tagtcgacct tgccgccgtg agcatcggct atactctgca cggccttgtc ttttgtttcg
    10201 ggcactgagt cggtgccaag gaattcgagt agtacttttt cagggtcggt atcttcgctg
    10261 ttgtcggcag ctgtcgctgc agcaggggga gcttcatctt tgttcgcaga gatatcttca
    10321 aattgcagct gtgctcccgt ggcgggcgct tgtcccagcg gcagcgtgaa gtcgagcggt
    10381 gagtaagaag agcgtttctg gcgccgcagt attttaccaa gcgactcaac gacgatttcg
    10441 tctttgttag ggtcgcgacc gatctgctgt ttcagcagcc gcacggcttc ggggttgtca
    10501 gattcaccga gcgcgcgaat ttcttcgcgg gtgagaccac tgaattcctg cgcgttgctg
    10561 cgcgcggcgt tgatgatagc ctgatcaatc tgggcgcggt tgctgtcatt gcgctgctgc
    10621 atgagccctt ctgctgcctc agcgcgcata tagcgattgg tgaggttttc agtcagtagc
    10681 acttcagagc gcggtgacgg aatcttacca acctcgcgca gcgccttctt ctgcatctcg
    10741 acatcgggat cttcgtcttt agggcgaatg gtacgctcga gtgcctgcca gcctttttct
    10801 tcgttggccg ctgcacgccg tgcagcttct tcgagctcta tctcgctgcg tacctgccgt
    10861 gccgcgcagt gcagcgttgt cagcagcaag agcgcaatgc cgccatacct caggtgttgc
    10921 agaaacgtgc gctccatact atattatcgg ccccggcggc gcattgttta tcgcaaaagg
    10981 ccgggctaag accaggtacc ggtggcagct aatgtgacat aattcggaaa aaatacgcag
    11041 gcgcgcgggc tagttgcagc acgaggaaag ctccccaccg cataagagtt tgaccctatg
    11101 tctaaaactc acgctttgac cccatgcagg cagttattca agtcgggagc aagcagtaca
    11161 aggtcgctca ggataccgta tttttcgcag agctgaccgg aaccgaaccc ggaaaagatc
    11221 tcactattga ctcggtgctc tcggtcaaca ataacggcca ggtaaaaatt ggccagccgt
    11281 tcgtttcagg cgcatcggtt acagcaaaag tactcgaaga agtaaaaggc ccgaaaatcg
    11341 ttggtgtttt ttaccgcacg aagaaacaca accaccgtaa atggggtcac cggcagaaat
    11401 accacaaact gcaagtaacc tcgatcaagg cctgattcgt ctacgctgcg ccgcaggcgc
    11461 agcgcattac cagcatttct acttttctgc gcgtacacgc tctttagcgt gacggctgtg
    11521 ctacagctaa taccgggggc agggttgtga agtttgtcca ttacctgaaa agattctacg
    11581 aaatctttat ccggggtaag attacgctct acctgctgct catcgtcgcc tatttcggcg
    11641 tgcgtacgtt cctggcgtac aaccgttgga acgacgaaga accggcaata ctacagaaac
    11701 tcactctcta caagcagcag ttggacaggc tgcgctccgg cgccattgcc gaagataccg
    11761 tggcttcgat cgaacccggc gcggttgcga ttccgtcgaa agtttttgac cgccacaaca
    11821 agctggtggg agagttttac accgaacgcc gtagcctgct caactacgaa cactttccgc
    11881 cgtttgtcac cgacgcgctg atggcgagcg aagaccgcag gttctacgaa caccacggca
    11941 tcgattacgc gggcatcatg cgcgccgtta tccgtaacct gctccacttc cgctttgcgc
    12001 agggcggttc aacactcacg cagcagctcg cgaaggtgct cttcactaac caggaaaaga
    12061 ccctcgaccg caaactgttt gaatatttct gcgcgcgcgc catcgaagaa cgctacagca
    12121 agaaagaaat actcgagatg tactttaacc tcatctacat ggggcacggc atctacggcc
    12181 ttgaaactgc ttcgcagttc tatttcaaca agtctgcccg ggctcttacc gtcggtgaag
    12241 ctgctgctct tgtgggagtt ataccgaacc ccaaggccta ttcgccgatg ggcgacctgc
    12301 cgcgttttct cgagcggcag ttggccgtga tgcgcagcat ggtggcgatc ggtaaactga
    12361 ccgaagacaa actcaagaat gagcgccggc gctttttcca ggcatggcga atcgccgcaa
    12421 aagacagcaa acaatcaggt attggcgatt ttcccgaccg gatgacggga aaaaattatg
    12481 ccccgttttt cctcgattac ctgcggcaga agctcaccgc gcagtttagc caggatgccc
    12541 tgacacgcag cgggctgcgc atctatacca ctctcgacct ggggcggcag attgctgcgc
    12601 agaaagcggt aaaggcagcc gtagagctac agaaacagca ctacgagaag ctcctcgccg
    12661 gcgcgcgcaa aaagaacaat gctgcgaaga tacaggaata cgaaaaagcg ctggcaacca
    12721 ccaatgcggc tttcatcagc attgaaccca aaaccggcta catcctaacg atggtcggcg
    12781 gcggtgaatt ttcatcgcag aaccagttta accgcgccct caaggccgaa cggcagattg
    12841 gttcgaccat taagccgctg atctactacc tggcgctgca gcgacagatt ctgacacccg
    12901 cctcgctgat tgccgacgaa ccgctgcagg actaccagaa ctacgacggc aaatttctgg
    12961 ggccgatcac gctgctcgat gcgctgaaac tcagtcgtaa cccgactgcg atcagggcgc
    13021 tgcaaaaact cgactttgat gacttgcgga gtctttttca cgaagtgctg cagctgcctt
    13081 acagcgacct cgagaagaag ataccacgcg agctgggtgt agcgctcggc agttcatcgt
    13141 ttaccccgct gcagctcgcg cagatgtatg cgacgatgct caaccagggc cggcgggtcg
    13201 agccgcgcga cctgctgcgc atcgaagaca gccaggggcg gcaaatctg
//
LOCUS       DAOQJF010000044        13084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2322_length_13084_cov_4.789930, whole genome shotgun sequence.
ACCESSION   DAOQJF010000044 DAOQJF010000000
VERSION     DAOQJF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_13084_cov_4.789930"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLF85_03840"
     CDS             complement(<1..699)
                     /locus_tag="PLF85_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18819.1"
                     /translation="MNLPGQPTSSETAKIRKIIDHTQLHQIAERLFARFPIHIVENNQ
                     KNAARALGYSHPHLEILHQLPPGPSRTLLLVKDDHSMMLECSVIGRSERSTEFVKPLR
                     LFLSKRGIRHENRVAVSGSDLAGQVRNVIPHPEFYRVNAGSNPVRDTLFSQYSTALKG
                     LIPETVVKIELQKSNRLSVRMKKLQDFNLPTFAPDMMRQRQGDEQSVVAMPHSELEQV
                     LRADGLPGDFIGEIC"
     gene            869..2203
                     /locus_tag="PLF85_03845"
     CDS             869..2203
                     /locus_tag="PLF85_03845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE18820.1"
                     /translation="MSGFSQFNFSPTLTDNITRAGFSEPTPIQREAIPQALEGHDLLA
                     LAQTGTGKTAAFSLPMLQRLLAGPRKRVRALVVAPTRELAEQIHEVILTLGKSCNLRS
                     MTIYGGASMNRQLGELRRGVDIVVACPGRLLDHMQQRSIDLSGVEILVLDEADQMFDM
                     GFLPGIRKIVAALPQKRQTMLFSATMPDAIRKLSMEILDNPRKVELERGPVATISHAL
                     YPVSHEQKTALLLHLLKDAGSDPILVFTRTKHKATRLADQLGKAGYTAAALQGNLSQN
                     QRQRSLDGFRSGKYQILVATDIAARGIDVNNISHIINYDIPATSETYIHRIGRTARAT
                     KSGDAYTFVTGEDKATVRDIEKALGKPIEKRMLDDFDYKGTLPVITSQPVGQPQAAPR
                     AKPARAAASQQRPADKRPPQKGPAQARPAAEGERSKAAGKPRRRRFKGFGKK"
     gene            2252..3007
                     /locus_tag="PLF85_03850"
     CDS             2252..3007
                     /locus_tag="PLF85_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE18821.1"
                     /translation="MKSPVAFITGASSGFGRAIAERFAAAGMQLVLLARREDRLHELS
                     KCLSVPCHTIACDVRDGDALRAAVAAIPGDFGRIDILVNNAGLALGLEPAHRTEWSDW
                     QQMIETNCTALALLTRLIVPGMVERRSGHIVLMGSVAGHYAYPGGNVYGASKAFVEQF
                     ARGLRADLSGQGVRVTHIAPGLAGGSEFSEVRFHGDREKAAAVYTGTEPLLPRDIAEC
                     VFWAVNQPPHVNINSIELMPTCQASGPLQVHRA"
     gene            complement(3004..3621)
                     /locus_tag="PLF85_03855"
     CDS             complement(3004..3621)
                     /locus_tag="PLF85_03855"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNE18822.1"
                     /translation="MKLYLHPVNPEARLVKQLAAMLKDGALLVLPTDTVYAFVTLAQS
                     KNSITQIQRIKNLPNHKALTLYCRDFSQMSGYVRSQGNQLFRLMKEILPGPYTLIFDA
                     AKNLPHYAVTKQNTVGIRIIEHPLIAALLAQTDLPLMGSSLDLPPEEMHDFEEIADRY
                     ENRVGAVVSCGDVPGQYSTILDVRDWPPTLIREGLGVVPDGVVRD"
     gene            complement(3664..4266)
                     /locus_tag="PLF85_03860"
     CDS             complement(3664..4266)
                     /locus_tag="PLF85_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18823.1"
                     /translation="MKKIKIIAMFAALTAFSAPAFSIAQIGVQGNYINDTFSSGTKET
                     GFGVGGFARFTVGLPMLVTFGFGPYIDYTTLKSDKTGLADQKQARIGGELVVYADIIG
                     NLIGLTPYGRFGFGYEGNSRSLTVGTTTGDILYYGTGGHTIFGLTMKVVPMVYLFAEG
                     GAQWSSLTASVPDALKSSGLGLSDITTTGWRASIGAMLWI"
     gene            4410..5393
                     /locus_tag="PLF85_03865"
     CDS             4410..5393
                     /locus_tag="PLF85_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNE18824.1"
                     /translation="MTVYQKNFPPGEWQKHFIPFEEDASQRSWLMPYLFIHGRNPGHK
                     VAIIAAIHGDELNGVRILHELAALLEGAPINGSLCLIPVANLMGYANHSRYLPDRRDL
                     NRMFPGLKDGSEGARLAHFLWTNFIAQADYVVDLHSGSYNRWNYPHLRGDMKDEAMRE
                     WVRSLEGQVVLTSAGVAGSLRKEAGKIGKPVFLVEAGETARFEKRILRMGTRLLLQLL
                     VSLDTIGRDVLAQVSARLDAEAPALSHPTGYYGKAVWQRATHAGLFMPQVEPGDRVRV
                     GQPLGFITDLFGETRAEILSEQEGFVLGMHLHPQVVPGRALFHVAYDFKEL"
     gene            5472..7751
                     /locus_tag="PLF85_03870"
     CDS             5472..7751
                     /locus_tag="PLF85_03870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE18825.1"
                     /translation="MNLEQYLDELKRTEGENIRALIERPEKPARYAELPASLGPEIRA
                     ALASRGIEKLYSHQREALDLYLAEKDYVVTTPTASGKTLCYLLPILHSKLKNPKGKHL
                     FMFPTKALAQDQLAAYRGYADSLGMSWNVNTFDGDTPEEERRAVRKAGDFILTNPDML
                     HSGIMPHHTIWKSFFENLETIVIDEMHTYIGVFGSHVANVLRRLNRLLEHYNAKPRYI
                     FCSATIANPAELAHQLAGREFNAVTESGAPEGKKYFLFYDPPFYEKLQVSESPYKAAA
                     RLGSGLIRNSIPTIFFARSRNRVEILTQFLRARLPDSLAHKVRGYRGGFLPLERRQVE
                     RSLREGQTLGVVSTNALELGIDIGALNAVVSIGYPGRLSSLYQQFGRAGRKKEPSLAV
                     LVASPSALDQYLMRNPEYLFESQGEAAIVNPDNLLIYQDQLKCAAYELRFNAGDVFGN
                     YPVDDFMDQLTAERVIVEKGGVYFWSSETYPAANVSLRSAATDNFVIVDKTDTGNEKV
                     IGEIDYFSAPLFIHDEAIYMHAGRHYFVNALKWEERRAEVTLVKSDYYTDAHEKVHRS
                     ILHLDEKREEGRLSLHWGEVTLRRQAYLYKKIKIETSENLGWGQIHTPEIEMHTQGAW
                     FDIDTKALPDLPPNFMSALLTRFAYLLRQLAPLVALCDRRDISVSSSYRDVQFGDHAI
                     IFHDNYPGGVGLSHKLVANHEALFALAAETVGKCSCEAGCPSCIGVWDAKLEGEAEQP
                     EDLKTPVKVLLQKLLVDAT"
     gene            7772..8794
                     /locus_tag="PLF85_03875"
     CDS             7772..8794
                     /locus_tag="PLF85_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18826.1"
                     /translation="MKYFVTAALVLAACSKPVDLKQEEAKYDAYIAGLPELKEVPRSG
                     KVVLKVHYVERPQLPALDTSQREDLYRLTEDLVIKTLGYDLKIEETIVYRAKDFLKAV
                     APRFKASPQRYPALAYQISFFAADRDARVLDAIKPALAKHPAERIAKYLGEHKTPEAA
                     TRFFIERLGAIYGETDYSGKAILGPANADEEVLFSYGHWSSILQGEREADFILTNTGI
                     IGADTGMPLYVIARGGVTSAFVENNAYRPFQGVGVIGLYPFLSDGAVFRAQRGDLTIE
                     QKIETIAWIWVHELGHLLLKKDENYAFEDSIHRAAPDLHYYDWVRRVKASRNHRSDQV
                     PMMKKF"
     gene            complement(8795..10135)
                     /gene="mtaB"
                     /locus_tag="PLF85_03880"
     CDS             complement(8795..10135)
                     /gene="mtaB"
                     /locus_tag="PLF85_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNE18827.1"
                     /translation="MKEGSRTALIHFGCRLNQFESDGIKANLADNAHTLTQNLREADF
                     VVVNTCTVTNKADYKNRAAIRRAHRLNPQAKIVVTGCYATTDAEELRSLPGVYRVVSN
                     DQKHRIAEVLQGGEVTAQASNRFFFSANHDLKSRATLKIQDGCNKSCSYCKIPQARGR
                     GVSRDFAETLDSARELIDAGFGELVLTGVNIGWYEDKGKSFHALVESLLDLPGDFHLR
                     ISSIEPGDVNERFAAFYQHPKMARFLHIPLQSGSRLVLKIMRRGYTPDNFMRRIEHVR
                     SVCPEVHIGTDVIVGFPAEGETEFKETLDFCRAADFANIHIFPFSKRRNTPIVDWLVK
                     RAERKSAGTAGEAEKRSYKEVNGAIIRERSATLSVLKKEMADAYIARMQGKKLFAVAE
                     RIENGKVHFLTENYLRGVLPVADYPSLRRGQRFDVEWDSALLGSNERTAASFIG"
     gene            complement(10132..10902)
                     /locus_tag="PLF85_03885"
     CDS             complement(10132..10902)
                     /locus_tag="PLF85_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HNE18828.1"
                     /translation="MHRTLKALFLVTPLALGAQQTDATYQKALAYFEQRNYDACLTAI
                     RPAIKESDENADARVLAAHCHAGKKNYADAVAHLRAVAEEAPERTDIKEDIVALLIEQ
                     GKFRDARRAGYRFSEELKDSEKPVPASLTLLVARAELGYGKPQNALTLARDVKKSDDA
                     TVKYGGLITETRALIALGNFSEADIALSFAESMRESDLHPLLRAMIEESKWIQQKYPK
                     DQRAQIVAAYEKLARSEDATIRAAVQKNIERVKAAKAN"
     gene            10992..11831
                     /locus_tag="PLF85_03890"
     CDS             10992..11831
                     /locus_tag="PLF85_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18829.1"
                     /translation="MLIEVQNDWGVTLWLLTSDERRPHLGGNKYHKLAGYVNQARSQN
                     VQHLLTMAGAHSNHLRAFAALVAMGEFSGTALIRGEELNDPARHSAEIREAIAAGVNC
                     VFVTRGLYRQLRAAHDASEVQKLVAQGELPRSVFIPEGGHGLPGTVGVAAWAAQAAGF
                     SEVYLACATGTTCAGFLLGTTAPTRIVGVSVVRNERSVMETIGMLAGNEAQRFTLLTE
                     YDQGGFARVYPGHAEFCNRLSAAWGVTVDPVYVGKAAAAIADRAQKGLVTGQPLLVYT
                     YNE"
     gene            complement(11813..12277)
                     /locus_tag="PLF85_03895"
     CDS             complement(11813..12277)
                     /locus_tag="PLF85_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNE18830.1"
                     /translation="MLMPANPLMSHQNAPSAVLLIRGGRIAASMLEAKLSKLGLTVPY
                     ATILTALLEHGSKSATDLCILTMRERANMSVLLGKLRKIQYVSESQNPLDARSQLISL
                     TDKGRELAVECKQVTEEVSGIIDNFLSQQHETPEKFRSALAEFLGKFHSLYV"
     gene            complement(12279..>13084)
                     /locus_tag="PLF85_03900"
     CDS             complement(12279..>13084)
                     /locus_tag="PLF85_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE18831.1"
                     /translation="ALLAEEYNKSQKKYEAKVGGGGSKAGIEDLTLSRIDIAMTSNEI
                     KETHLEALADISKFEKVEIGYDGLAIVVHNTNKVKQIHLSQYAGILSGKITNWKELGG
                     DDMAILPVVRNSNSGTEAFVREHVLRRKDLGEAVYAVFRNTEYTGSATVKRDNREILE
                     FVATNPGAIAYMGVGVAKSEGKNRIRILDYALAKEGPFLQPTVENIQAGKYRLSRALS
                     LVYVPDNARKDAFIAYALSEAGQKKILQYEYMQAAPNTVVVIEKRTKGN"
BASE COUNT         2761 a         3642 c         3760 g         2921 t
ORIGIN      
        1 acagatttcc ccgataaaat caccaggcag cccatcggcc cgcagtacct gctcgagttc
       61 gctgtgaggc atcgcaacaa ccgactgctc atcgccctgg cgctgccgca tcatgtcggg
      121 cgcaaaagtt ggcaggttga aatcctgcag tttcttcatg cgtacagaga ggcggttgct
      181 tttttgcagt tcaatcttca ccaccgtctc gggaattaat cccttcagcg cggtggagta
      241 ttgactgaaa agtgtgtcgc gcaccgggtt gctgcctgca ttgacgcggt agaattcagg
      301 atgcggaatc acgttccgca cctggcccgc gaggtcgctg ccgctgaccg cgacgcggtt
      361 ctcgtggcgt atgccgcgtt tactgaggaa taagcggagg ggttttacga actcggtgct
      421 gcgctcgctg cggccaatca cactgcattc aagcatcatc gagtgatcgt ccttcaccag
      481 cagcaaggtg cgtgacggtc caggtggaag ctggtgcaaa atttcgagat gcggatgcga
      541 ataaccaagc gcgcgcgcgg cattcttttg gttattttct acaatatgaa taggaaaacg
      601 ggcaaacagc cgctcagcaa tctggtgcag ctgagtatga tcaatgatct tgcgtatttt
      661 tgctgtttcg ctgcttgtag gctgcccggg aaggttcatg ataagtgtcc atgaccgtag
      721 tctgtaaaaa ctccgtggcg ttaataaatt attgttatgc tgcataagta gaactactta
      781 cgaaagcaga aacgttttcc cggatttttt ttggcacgcc cataccttgt tgtgctgtac
      841 gcgttgtctt gcgaacagtc ttaggcagat gtccggtttt tcccagttta atttttctcc
      901 gacgcttact gacaatatta cgcgcgcagg gttctccgaa cccaccccga tacagcgtga
      961 agctattccg caggcgctcg aaggccacga cctgctggcg ctcgcgcaaa ccggtactgg
     1021 taaaactgct gccttttcac tgccgatgct gcagcgcctg ttggcaggcc cgcgaaaacg
     1081 tgtgcgtgca ctggtcgttg caccgacacg cgaactggcc gaacaaatac atgaagttat
     1141 tctcactctc ggcaagagct gcaacctgcg ctcgatgacg atctacggtg gtgcgagcat
     1201 gaaccgccag ctcggcgaac tcaggcgcgg ggttgatatt gtcgtcgcct gtccaggcag
     1261 gctgctcgat cacatgcagc agcggagcat cgacctgagc ggggtggaaa ttctcgtgct
     1321 cgatgaagcc gaccagatgt ttgatatggg gtttctgccg ggcatccgca agattgtcgc
     1381 ggcgctgccg cagaagcggc agacaatgct cttttcagcc accatgcccg atgcgatccg
     1441 caagctgagc atggagatac tcgacaatcc ccgcaaggta gaactcgaac gcggccccgt
     1501 tgcgacaatc agccatgcgc tgtacccggt ctcgcatgag cagaagacgg cgctgttgct
     1561 gcacttactc aaagatgctg gcagtgatcc cattctcgtc tttacccgca ccaagcacaa
     1621 agcgacgcgg ctcgccgacc agctgggcaa agcaggttat accgccgctg cactgcaggg
     1681 caacctgtcg caaaaccagc ggcagcgttc gctcgatggc ttccgctcgg gcaagtacca
     1741 gatactcgtc gcgaccgata tcgccgcgcg cggcattgac gtgaacaaca tatcgcacat
     1801 catcaattac gacatacctg ccacgagcga aacctacatc caccgcatcg gcagaaccgc
     1861 tcgggcgaca aaaagcggcg atgcctatac tttcgtgacc ggcgaagata aagcgaccgt
     1921 acgcgacatc gagaaggcgc tgggtaaacc gattgaaaag cgtatgctcg acgatttcga
     1981 ctacaagggc acactgccgg tgattaccag ccaaccggtg ggccagccgc aggcagctcc
     2041 gcgggcaaaa cccgcgcgcg ccgctgcgtc acagcagcgc ccggccgaca aaaggccgcc
     2101 gcagaaaggc cctgcccagg cccggccagc cgcagagggt gaacgcagca aagcagctgg
     2161 caaaccccgc cggcgtcgct tcaaaggttt cggtaaaaag taatatcctc cggctcttga
     2221 caccttgtgc ggtgttaaga accgttggaa catgaaatcc cccgttgcct ttatcaccgg
     2281 tgcttccagc ggctttggcc gcgccatagc cgaacgtttt gccgcggcag gtatgcagct
     2341 cgtgctgctg gcgcgacgtg aagaccggct gcatgaactc agcaaatgcc tctcagtacc
     2401 ctgccatacg attgcctgcg acgtccgcga cggcgatgcc ctgcgtgcgg cggttgctgc
     2461 aattccgggg gatttcggac gcattgatat cctcgtcaac aatgcaggac ttgccctcgg
     2521 gcttgagccc gcgcaccgca ccgagtggtc agactggcag cagatgatcg agacgaactg
     2581 caccgcgctc gcgttgctca cccgccttat tgtgccaggt atggttgaac gccgcagcgg
     2641 ccatatcgtg ctcatgggtt ccgtggctgg ccactacgcg tacccgggcg gtaatgttta
     2701 cggggcaagc aaggccttcg tcgaacagtt cgcgcgcggt ctcagggcag atctttcagg
     2761 gcagggcgta cgcgtgacac acatcgctcc tggtcttgca ggaggcagtg aattttctga
     2821 agtgagattt cacggcgatc gcgagaaagc cgcagcggtc tacaccggaa cagaaccgtt
     2881 gttaccccgg gatattgctg aatgtgtgtt ctgggctgtg aaccagccgc cgcatgtgaa
     2941 catcaacagc atcgagttga tgccgacctg ccaggcctca ggtccgctgc aggtgcaccg
     3001 cgcctaatct cttactacgc cgtcgggaac gactcccagc ccttcgcgga tcagtgtcgg
     3061 tggccagtca cgcacatcga ggatcgttga atactgcccc ggtacgtcgc cacagctcac
     3121 cacggctccg acacgattct cataacgatc ggcgatttct tcgaaatcgt gcatttcttc
     3181 gggcggtaaa tcgagcgaac tgcccatgag cggcaggtcg gtctgcgcca agagcgcagc
     3241 gattaaggga tgttcgatga tacggatgcc cacggtattc tgtttggtca ccgcgtagtg
     3301 cggcaggttt ttcgccgcgt caaaaatcag cgtgtagggt ccgggaagaa tctctttcat
     3361 tagtcgaaag agctggttgc cctgcgagcg tacatagccg ctcatctgcg aaaaatcgcg
     3421 gcagtagagc gtgagcgcct tgtggttggg caggttcttg atacgctgta tctgcgtgat
     3481 gctgttcttg ctctgtgcca gggtgacaaa ggcatagacg gtgtctgtcg gcagaacgag
     3541 cagcgctccg tctttgagca tggctgcgag ctgtttgaca agtctcgctt caggatttac
     3601 agggtgaaga tagagtttca tgaaattgtg cccggcctca tggccaggca caagcaaaaa
     3661 ggtttagatc cagagcattg cgccgatcga cgcacgccag cccgtggtgg tgatgtcaga
     3721 gagtcccaga ccgcttgatt tcagcgcgtc aggcactgaa gctgtcagtg agctccactg
     3781 cgctccacct tcggcaaaca ggtaaaccat gggtacgact ttcatcgtca gaccaaagat
     3841 ggtgtggccg ccggttccgt agtaaaggat atctccggtt gtcgttccaa cagtaagcga
     3901 acgcgagtta ccttcataac caaagccgaa acgcccgtag ggtgtcaggc caatcaggtt
     3961 gccgatgatg tcggcgtata ctacaagctc accgccgata cgtgcctgtt tttggtctgc
     4021 caggccggtc ttgtcgctct tgagtgttgt atagtcgata taggggccaa agccaaaggt
     4081 gacgagcatg ggcaggccga cggtgaagcg ggcaaaacca cccacgccga agccggtttc
     4141 ttttgtgccg ctcgaaaagg tatcgttgat gtagttaccc tgaacgccga tctgtgcgat
     4201 tgagaaagct ggtgcgctga aagcggtaag tgccgcgaac attgcgataa tctttatttt
     4261 cttcatgttc ctccgatatt ccggggtgaa cccggtgttc tctcttatca gacgcccgag
     4321 cgggccatgg cggtcaatca aattcgctgg atttttgact tgcctcgcca tccatccacc
     4381 cgcaagagac ataattcccg aggctggcaa tgaccgtcta ccagaaaaac ttcccccccg
     4441 gcgagtggca gaagcacttc ataccctttg aagaagacgc ctcgcagcgg tcatggctga
     4501 tgccttacct ctttatacac ggccgaaatc ccggccacaa ggtcgccatc atcgcggcca
     4561 tccacggcga cgagctgaac ggggtacgaa ttctgcatga acttgccgcg ctgctcgagg
     4621 gggcgccgat caacggttcg ctctgcctga tacccgtggc gaatctcatg ggttatgcca
     4681 accattcgcg ctatctgcca gacaggcgcg acctcaatcg gatgttcccc ggcttaaaag
     4741 atggctccga gggggcgcgg cttgcgcact ttctgtggac aaattttatt gcgcaggcag
     4801 actacgttgt cgacctgcat tcgggcagct ataaccgctg gaactacccg cacctgcgcg
     4861 gcgacatgaa agacgaagcg atgcgcgaat gggtcaggtc gctcgaaggg caggtggtgc
     4921 tcaccagcgc cggtgttgcg ggcagtttgc gcaaagaggc aggcaagatc ggtaaacctg
     4981 tcttcctggt cgaggccggc gaaaccgcgc gctttgaaaa aagaatactg cggatgggta
     5041 cgcggctttt gctgcaactg ctcgtttcgc tcgacacgat tggccgcgac gtactcgcgc
     5101 aggtttcagc ccgcctcgat gccgaagcgc ctgcgctatc gcacccaacc ggttattacg
     5161 gcaaggcggt atggcagcgc gccacgcacg caggtctgtt tatgccgcag gtcgaacctg
     5221 gcgaccgcgt gcgcgtcggt caaccattag gattcattac cgatctgttc ggcgaaaccc
     5281 gcgcagagat tctttctgag caagagggtt ttgtcttagg catgcacctg cacccgcagg
     5341 ttgtgccagg cagagcatta tttcatgtgg cgtatgattt caaagagctc tagtccccac
     5401 cccgaccttc ggtcacccct ccccgcatgc ggggaggggt tgagggaggg gacacgtagc
     5461 ggagcaaagg catgaacctg gaacaatacc tcgatgagct gaaacgcacc gagggcgaaa
     5521 atatccgcgc tttgatcgag cgcccggaaa agccagcacg ctatgccgag ctgccagctt
     5581 cgcttggacc agagatacgc gctgcgctcg cctcgcgcgg tatcgagaag ctctattcgc
     5641 accagcgtga ggcactggat ctctatcttg ctgagaaaga ttatgttgtg acgacaccga
     5701 ccgcctcggg caagacgctc tgctacctgc tgccgattct gcactcgaag ttgaagaacc
     5761 ctaagggcaa gcatttgttc atgttcccga ccaaggcgct ggcgcaggac cagctggctg
     5821 cctaccgcgg ctatgcagat tcgctaggca tgagctggaa cgtgaatacg ttcgatggcg
     5881 atacacccga agaagagcgg cgcgcagtgc gcaaggcggg agatttcatt ctgacgaacc
     5941 ccgacatgtt gcactcgggc attatgccgc accatacaat ctggaaatca ttctttgaaa
     6001 accttgagac cattgttatc gacgagatgc acacgtacat cggggttttt ggttcgcatg
     6061 ttgccaatgt gctgcgcagg ttgaaccgcc tgctcgagca ctacaacgcc aaaccccggt
     6121 acattttctg ctcggcgacg atcgccaacc cggcagagct cgcccaccag ctggcgggca
     6181 gggaattcaa cgcggtgact gaatcgggcg cccccgaagg caagaagtac ttcctgtttt
     6241 atgatccgcc gttttatgaa aaattacagg tgtcagagtc gccgtacaaa gctgcagccc
     6301 gtttgggttc gggcctgatc aggaattcaa tacccacgat cttttttgcg cgctcgcgca
     6361 atcgggtcga gatcctgacg cagtttctgc gtgcacgcct gcccgacagc ctcgcgcaca
     6421 aggtgcgcgg ttaccgcggc gggtttctgc cgctcgagcg ccggcaggtc gaacgttcgc
     6481 tgcgcgaggg acagaccctc ggtgtcgttt caaccaacgc gctcgagctt ggtatcgata
     6541 tcggagcgct caatgccgtc gtgtcgatcg gttacccggg tcgcctgagc tccctctacc
     6601 agcagtttgg tcgcgcaggg cgcaagaaag aaccgtcgct cgccgtgctc gtggcatcgc
     6661 cttctgcgct tgaccagtac ctgatgcgca accccgagta cctgttcgag agccagggcg
     6721 aagcggcgat cgtcaacccc gacaacctgc tcatctacca ggaccagctc aagtgcgcgg
     6781 cgtacgaact gcgttttaac gcaggcgatg tcttcggcaa ttacccggtc gatgatttta
     6841 tggaccagct gacagcagag cgtgtcattg tcgaaaaggg cggagtgtat ttctggagca
     6901 gcgaaactta cccggctgca aatgtttcgc tgcgttctgc ggcgaccgac aattttgtga
     6961 tcgtcgacaa gaccgatacg ggcaatgaaa aggtcatagg cgagatcgat tatttttcgg
     7021 cgccactttt tattcatgat gaagctatct atatgcatgc gggccggcac tactttgtga
     7081 acgcactcaa gtgggaagag cgccgtgccg aggtcaccct cgtcaagtcg gactactaca
     7141 ctgatgcgca cgaaaaggtg caccgcagta ttttacacct cgacgaaaaa cgcgaagagg
     7201 gccggttgag cctgcactgg ggtgaggtga ctctgcggcg gcaggcctac ctttacaaaa
     7261 aaatcaagat tgaaacttcg gaaaaccttg gctgggggca gatacacacg cccgaaatcg
     7321 agatgcacac gcagggcgcc tggtttgata tcgacaccaa agcgctgcca gacctgccgc
     7381 caaattttat gagcgcactg ctcacgcgct ttgcctacct gctgcgacag ctcgcgccgt
     7441 tggtggcgct gtgcgaccgc cgcgatatct cggtttcttc gagttaccgc gatgtacagt
     7501 ttggcgacca cgcgatcatc tttcacgata actatccggg tggggtgggg ttgtcacaca
     7561 agctggttgc gaaccacgaa gcactttttg cactggctgc cgaaaccgtg ggcaagtgca
     7621 gctgcgaggc cggttgcccg tcatgcatcg gggtatggga tgcaaagctc gagggcgagg
     7681 cagaacagcc tgaagacctg aagacaccgg tcaaagtgct tttgcagaaa ttgcttgttg
     7741 acgcaacata agacacacca taacggcggc tatgaaatat ttcgtaaccg ctgcgctcgt
     7801 actggctgcg tgttcaaagc ccgttgatct gaagcaagaa gaagcaaaat acgacgccta
     7861 cattgcaggg ctgcctgaac tcaaggaagt gccgcgcagc ggcaaagtcg tgctcaaggt
     7921 gcactacgtc gagcggccgc agttgcctgc gttagacacc agccagcgcg aagatctcta
     7981 ccggttaacc gaagacctgg tgataaaaac gctcggttac gatctaaaaa tcgaagagac
     8041 gattgtctac cgcgcgaaag attttctcaa ggccgtagcg ccgcgcttca aagcttcgcc
     8101 gcagcgttac cccgcgctgg cataccagat ttcttttttt gcggcagacc gcgacgcgcg
     8161 ggtgctcgat gcgataaaac cggctcttgc gaagcaccct gccgagcgta ttgcaaaata
     8221 cctgggcgaa cacaagacac ccgaagcggc gacgcgcttt tttatcgaac gcctcggcgc
     8281 aatatacggc gagactgatt attcaggaaa agccattctc ggcccggcca atgccgatga
     8341 agaagtgctg ttctcttatg gccattggtc gagcatactg cagggcgaac gcgaggcaga
     8401 ttttattctc accaataccg gcatcatcgg ggctgatacc ggaatgcctc tttacgttat
     8461 tgcgcggggc ggtgtgacca gcgcgttcgt cgaaaacaat gcgtaccgac cgttccaggg
     8521 tgtgggagtg attggccttt atcctttcct gtcagacggg gcagtatttc gcgcgcagcg
     8581 gggcgacctg actatcgagc agaaaattga aacgattgcg tggatctggg tgcacgagct
     8641 gggccacctg ctgctcaaga aagacgagaa ctatgccttc gaagattcta tacacagggc
     8701 ggctcccgac ctgcactact acgattgggt caggcgcgtg aaagcatcgc gcaaccaccg
     8761 cagcgaccag gtgcccatga tgaaaaaatt ttaatcatcc aatgaaagaa gctgcggttc
     8821 tttcattgga tccgagcagt gcagagtccc actcaacatc gaagcgctgg ccgcgcctga
     8881 ggcttgggta gtcagctacc ggcagtacgc cgcgcaggta gttttcggtt aagaaatgta
     8941 ccttgccgtt ctcgatgcgt tctgcgacgg caaagagctt tttgccctgc atgcgcgcga
     9001 tgtaggcatc ggccatttct ttcttgagta ctgagagagt cgccgagcgt tcgcggatga
     9061 tggcaccgtt gacctctttg tagctgcgtt tttcagcttc tcccgcggtg cctgcgcttt
     9121 tgcgttctgc acgtttgacc aaccagtcga cgatcggcgt attgcggcgc ttggaaaatg
     9181 ggaaaatatg gatattggca aagtcggctg cgcggcaaaa gtcgagtgtc tctttgaact
     9241 ccgtctcacc ttctgcgggg aaacccacaa tgacgtcggt gccgatatgt acctcgggac
     9301 aaacactgcg cacgtgttca atgcgccgca tgaaattatc cggggtatag ccgcggcgca
     9361 tgatcttcag caccagtctt gaacccgact gcagcggaat atgcagaaaa cgcgccatct
     9421 tcgggtgctg gtagaaagcc gcaaagcgtt cgttgacatc acccggttcg atcgaactga
     9481 tacgcaggtg aaaatcaccc ggcaggtcaa gcagcgattc gacgagcgca tgaaagctct
     9541 tacccttgtc ttcgtaccag ccgatgttga cgccggtaag taccagttcg ccgaaaccag
     9601 cgtcgatgag ctcgcgggct gaatcgagcg tctctgcaaa atcgcgcgaa acgccgcgcc
     9661 cgcgcgcctg tggaatcttg cagtagctgc agcttttgtt gcagccgtcc tgtattttta
     9721 gtgtagcgcg gcttttgagg tcatggttgg cagaaaagaa aaaacggtta gatgcctgtg
     9781 cggtcacttc gccgccctgc aagacttcgg caatgcggtg cttctggtcg ttcgaaacga
     9841 cgcggtagac acccggcagt gaacgcaatt cttcagcgtc ggttgtcgcg taacaaccgg
     9901 tgacgacaat ttttgcctgc gggttgaggc ggtgcgcacg ccggatggca gcacggttct
     9961 tgtagtcggc cttgttggtg accgtgcagg tgttgacgac gacaaagtct gcttcgcgca
    10021 ggttctgcgt cagcgtgtgc gcattgtcgg caaggtttgc cttgatgccg tcactctcaa
    10081 actggttgag ccgacagccg aagtggatta gagctgtacg agaaccttct ttcaatttgc
    10141 cttcgccgcc ttgacgcgct caatattttt ctgtactgct gcacgtattg tcgcgtcttc
    10201 ggagcgggca agtttttcgt atgccgcaac gatctgtgcg cgctggtctt tggggtactt
    10261 ctgctgtatc catttgcttt cttctatcat tgcgcgcagc agcgggtgca ggtcagattc
    10321 gcgcattgac tcggcgaagc tcagggcaat gtctgcttcg gaaaaattac cgagagcaat
    10381 gagtgccctg gtctcggtga tcaatccgcc gtatttgaca gttgcgtcgt cgctcttctt
    10441 cacatcgcgc gccagagtga gtgcgttttg tggtttacca taacccagct cggcgcgggc
    10501 gacgagcagc gtcagcgagg caggcacggg tttttcgctg tctttcagtt cttcgctgaa
    10561 ccggtaaccc gcgcgccgcg catcgcggaa tttgccctgc tcgatcagta gggcgacgat
    10621 gtcttctttg atgtctgtac gttcaggcgc ttcttctgcc accgcgcgca ggtgcgcaac
    10681 cgcatcggca tagtttttct tgcccgcatg gcaatgagca gccagcacac gggcatctgc
    10741 attttcgtcg gactctttga ttgcggggcg aattgctgtc aggcaggcat catagttgcg
    10801 ctgctcgaag taggccagcg ccttctggta ggtcgcgtcg gtctgctgcg cgccgagcgc
    10861 gagcggcgtg accaggaaca gggctttcag tgtgcggtgc attaggggca atgcccggcg
    10921 ttcgagacag cgcgaaaagt cttaaaaggg gtagtcgtat agcgacagaa agccattatg
    10981 ctgaagctcc catgctgatc gaggtacaaa atgactgggg tgtaacactt tggctgctga
    11041 cgagcgacga acgccggcca cacctcgggg gcaataagta ccacaaactt gccggctacg
    11101 tgaaccaggc gcgttcacag aatgtgcagc acttgctgac catggcgggt gcacattcaa
    11161 accacctgcg ggcgtttgcc gcactggtag ctatgggaga attttcgggt accgcgctca
    11221 ttcgcggcga agagctcaat gacccggcca ggcattccgc tgaaattcgc gaggcgattg
    11281 cggccggggt gaactgtgtt tttgtcacgc gcgggctgta ccggcagctg cgtgctgcgc
    11341 atgatgccag cgaagtgcaa aaactggtgg cacaggggga gctgccccgt tctgtcttca
    11401 tacccgaggg tgggcatggc ctgcctggta ctgtgggcgt cgccgcttgg gccgcgcagg
    11461 ccgcgggctt ctctgaggtt taccttgcct gtgccacagg cacaacctgc gcgggatttt
    11521 tactcggtac tactgcgccg acgcgcattg tgggtgtctc tgttgtgcgc aatgaacgct
    11581 ccgtcatgga gactataggc atgttagcgg gcaatgaagc gcagaggttt acgctactca
    11641 cggaatacga ccagggcggc tttgcgcgtg tttaccccgg gcatgcagag ttttgcaacc
    11701 ggctgtctgc agcgtggggt gtgacggtag atcccgtcta tgtcggtaag gcagcggcgg
    11761 cgatcgcgga ccgtgcgcaa aaagggctgg tcaccggcca acccctgctc gtctatacgt
    11821 acaacgagtg aaacttaccg agaaactcgg caagcgccga gcgaaatttc tcgggcgtct
    11881 cgtgctgctg tgagagaaaa ttatcgatga tacccgaaac ctcttcggtc acctgtttgc
    11941 attcgacagc cagttcgcgt cctttatcgg tgagcgagat taactggctg cgcgcatcga
    12001 gcgggttctg cgattcagag acatactgta ttttcctgag tttgccgagt agcacactca
    12061 tgtttgcgcg ctcacgcatt gtcagtatgc agagatcggt tgccgacttt gaaccatgct
    12121 cgagcagtgc cgtcagtatc gtggcatagg gtactgtcag gcccagcttg gaaagtttcg
    12181 cctcgagcat cgacgccgca atgcgcccgc cacgaatcag cagaaccgcg ctcggcgcat
    12241 tctggtgaga catgagcggg tttgcgggca tcagcatctt agttcccttt tgtccgtttt
    12301 tcgatcacta ccaccgtatt gggtgctgcc tgcatgtact cgtattgcag aatttttttc
    12361 tggcctgctt cgctcagcgc gtaggcaata aatgcatctt tgcgcgcgtt gtcgggtaca
    12421 tacaccagtg acaatgcgcg cgacaggcgg tatttacctg cctgtatatt ctctactgtt
    12481 ggctgcagga acggcccttc tttggcgagt gcgtaatcga gaatacgaat cctgttttta
    12541 ccttcagact ttgcaacgcc gacccccatg taggcaatcg cgcccgggtt cgttgcaaca
    12601 aattcaagaa tctcgcgatt gtcgcgtttg acagttgcgc taccggtgta ctcggtattg
    12661 cgaaagaccg cgtagactgc ttcacccagg tctttgcggc gcaacacatg ctcgcgcaca
    12721 aacgcctcgg tgcccgagtt gctgttgcgc acaacgggca gaatcgccat atcgtcgcca
    12781 ccgagttctt tccagttggt gatcttgccc gagagtatgc cggcgtactg gctcaggtga
    12841 atctgcttta ccttgttggt gttgtgcacc acgatcgcca ggccatcgta gccaatttcg
    12901 actttttcga acttgctgat gtcagcgagc gcttcgaggt gggtttcttt aatttcattc
    12961 gaagtcatgg cgatgtcgat gcggcttagg gtcaggtctt cgatgccggc ctttgaaccg
    13021 ccgccgccaa ccttggcctc gtattttttc tgtgacttgt tgtactcctc ggccaaaagg
    13081 gcga
//
LOCUS       DAOQJF010000045        12954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2359_length_12954_cov_5.246143, whole genome shotgun sequence.
ACCESSION   DAOQJF010000045 DAOQJF010000000
VERSION     DAOQJF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2359_length_12954_cov_5.246143"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1162
                     /locus_tag="PLF85_03905"
     CDS             8..1162
                     /locus_tag="PLF85_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE18832.1"
                     /translation="MLRRALKIFGLALLAALGGMLALGWQAFGRTAEGERLERMRHSP
                     QWLGNSFENPQPLRNDFAGSISAMFKSGRFTAPVGAIPVERTNGSQYHAAPTSGLRVT
                     WFGHSSTLVEIDGARVLTDPVFSERVSPYPWLGPKRWYPPPVTFGDLPAIDVVVISHD
                     HYDHLDMYTVKLLLAGKAIFVVPLGIGAHLAYWGIPKERIVELDWWQETQVGRLRVVL
                     TPARHASGRWLVDFNKKLWGGFALIGKKNRVYYSGDTGLFPAMKEIGRRLGPFDLTMI
                     EVGQYNRSWPDWHIGPEQAVKAHRWVNGRTMLPVHWGLFVLAAHNWTEPIERVLREAE
                     PHGVRVATPKPGEAFEPAQAAADLRFVKRWWPAVEWQSAAEHPIISTQVE"
     gene            complement(1323..2843)
                     /locus_tag="PLF85_03910"
     CDS             complement(1323..2843)
                     /locus_tag="PLF85_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HNE18833.1"
                     /translation="MTQTVLLIHTTAAYTPDFNQSGAAALPEAFAAKLEALGLPVHLC
                     VADDSLRQRLEGLLPAAKLLPVQSEIAAVRALFAESGVNHIAICGGVFPLLDAELTQK
                     LHGIHTEYRADITYGENLPPGVAPHIVSRDLLESLEIMEASDADVATVGLRPFVEKNI
                     NQFHAEVHYEEPDLRVLRLDFSLATKRSVTKTAALLQHLPAGQGYTALKPAIETHPEI
                     LVTFPSYLELEFLSTAEHVSYFSPLKAIEQPRHMLSAAHFDKVLAYIGQGYGDTSVCA
                     SGLGEPLEHPDAVGYLTRLIEQPQVRYVFIETNGLHLDKLWPLATHAQAQKLRVIVML
                     NSLERYTDYSDAPLVTLDRVKANVQSLTGALTAAGRNAQELVYLQAFKVVENETEIDS
                     LYALAEDLGASFLLQKYNRYAGLLPERRVSDMTPLERYSCWHLRRDLFIRANGDIAYC
                     KQTVDQNKNSARGNLDKGTLAEIWQNQRNDFIANFRGQYPAHLPCATCDEYFTFNF"
     gene            complement(2853..3800)
                     /locus_tag="PLF85_03915"
     CDS             complement(2853..3800)
                     /locus_tag="PLF85_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNE18834.1"
                     /translation="MSARLLLLILTSAALSCASLAATVLADVVAVVASAPITSIDLSL
                     EMERMQKRKMPLKDKRSIKSHALDALIGRAIIDVIAREESVTISPERINNAIKKEMDM
                     RGILREDDFKKVVKRDMGVDFDTYKEELSRQLKTQQVMQLRVSVPNPTPSQVEEWYRQ
                     NKNRVGKKYTFRMIFIPVAGNELKVSNQMKAARAMGASSSAGFAKAATQYSAHGSKAR
                     GGLMVDLRLDQVAQIDPILAGAVNGTPMGTTSQIFVGANGGYYCVRVESARAIGLDEV
                     YDNVRAILYSQNEQIEYGRWLKEQRKKVAVTIYLKDYQE"
     gene            4161..4388
                     /locus_tag="PLF85_03920"
     CDS             4161..4388
                     /locus_tag="PLF85_03920"
                     /inference="COORDINATES: protein motif:HMM:NF021425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18835.1"
                     /translation="MSAWGLWHNRRWGYLAGIMAGSALVFLGLLDVSFNLQNARYSIG
                     LVDAAMNVLINAVCLVFGPLLVRSCWQKLQP"
     gene            complement(4404..5198)
                     /locus_tag="PLF85_03925"
     CDS             complement(4404..5198)
                     /locus_tag="PLF85_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE18836.1"
                     /translation="MRIILITGSTGFIGSHLADACLKKKYRVRALVMRGDPGEVGLKK
                     QRVEIVYGDLRDGPSLEAACKGVDIVFHCAAVVTDWAPRSLFQAVNIDGMEKLCRAAL
                     NARVQRFVELSTNDVFGLDESRVITEEAPLKKWGEPYADTKIAAEEIAWRYYQAHGLP
                     VTTFQQFCAAIAAALGAKAPRLHLPYFVAYGAALLMEFTWRILRLRSRPLLTTYTVKN
                     LGSRLRFSIERAERELGWTPKISYREGLARTLEWLKGLDRSNLKQK"
     gene            5333..5530
                     /locus_tag="PLF85_03930"
     CDS             5333..5530
                     /locus_tag="PLF85_03930"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNE18837.1"
                     /translation="MKTTLYLDDDLLARAQELSGIREKTQLVNRGLQTLVSQLASQRL
                     AQMGGALSSLKTPPRRRKNAL"
     gene            5520..5867
                     /locus_tag="PLF85_03935"
     CDS             5520..5867
                     /locus_tag="PLF85_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE18838.1"
                     /translation="MHSEFIADTSVWVNHFKRRGSSEFVSALRENRVLLHSLVYAELV
                     LGGIEKNEEADELLGLLRRPIEATYDEILEFIRVKKLAGRGVGWVDAALLASAALSGC
                     GLLTEDKALRRLT"
     gene            complement(5904..7097)
                     /locus_tag="PLF85_03940"
     CDS             complement(5904..7097)
                     /locus_tag="PLF85_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE18839.1"
                     /translation="MLRGVLSLTIIVTLSAVFALEVEDLRKQAQQKTDTVRSESQKKV
                     EKILARFAEFRSDLEKLKPGLDISLPVDTKNAGKMRLIDHNELTGIPEAPKVFAIVTQ
                     DYRLKTWHSDSDSQNLDRVRAGDKVEVVMVVNPRASTPLSNRSLSEVEGWALIRTATE
                     SEGYIPQMYLKNVPEQVQPSTKKKEAKYNISSSGLRMRSEPSLAGEFIQLVPAEAEVE
                     VIAYSASKDTIDGLSDFWAQANYAGKSGWLFNGYLRPVAQKPAPQPPSVGAGGFAMPV
                     DGRVNSKFGPRIDPVTKKSGDFHRGIDIMAPIGEPIKSAKAGEIYENTANKWWGNYII
                     VKHSENLFTYYCHQSRTKARKGQRVAAGELIGYVGRTGKATGPHLHFEVRVGKDPKDP
                     LQYLK"
     gene            7298..8095
                     /gene="folE2"
                     /locus_tag="PLF85_03945"
     CDS             7298..8095
                     /gene="folE2"
                     /locus_tag="PLF85_03945"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNE18840.1"
                     /translation="MAASLVDVQSSADTRRIAIDKVGIKGIRYPIQIRDKTGETQNTV
                     AELNMYVYLPQQFKGTHMSRFVEILERKKHLDVPTFKALLPEMLERLESHVGQIEMKF
                     TYFVNKAAPVSGVQSLMDYDVTFIGHCENGITDLRIKVVVPVTSLCPCSKEISQYGAH
                     NQRSHITITARLSELAEGANTLWIEDIIRLAEESASCELYGLLKRPDEKYVTEKAYEN
                     PKFVEDIVRDVAHRLNQDDRISHYVAEAENFESIHNHSAYALIEKHK"
     gene            complement(8192..8974)
                     /locus_tag="PLF85_03950"
     CDS             complement(8192..8974)
                     /locus_tag="PLF85_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18841.1"
                     /translation="MKKILAMLAVLSLIGAVHAQKKAKPAAKAAAKVDAAKADADAKA
                     KAAQDAANAKAADVQNKANEKLTSAAASVESKMSVAVWGGYNFASSTFFSDLEKTIKD
                     SETSAGFSTSKSPTQTKGGIAAGADLMLGGDFKYGLGLAYLQIYKLDWTNSEAGTTAK
                     ITSNIAFAPIHAQAQYIIGGLKLGVGAGISLGTGETKTEVTGSETTTTKVKGSSFSAS
                     ASVAYGFDFDGVSADVGVRYYAIFDDKMFHNIVPSVTIGYKF"
     gene            complement(9137..9769)
                     /locus_tag="PLF85_03955"
     CDS             complement(9137..9769)
                     /locus_tag="PLF85_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE18842.1"
                     /translation="MKNLLVVEDNKTVRQGLADLFKKNKEFGVSGTYGDLQAALADSR
                     ALHPDLIVLDLKLPDVRGTEAISGLREKYPEAKVVVYTAYENENEIIACMMAGANGYI
                     MKDTPPARFLSELEVIAQGGSTLTPRVAEKILRHFAPHKATESSLSKRELQVLNLISL
                     GLRYEDIAEELDISVHTVRHHIEKIYKKLNVTSRGQAVAQAVRAGIIRLE"
     gene            complement(9766..10938)
                     /locus_tag="PLF85_03960"
     CDS             complement(9766..10938)
                     /locus_tag="PLF85_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18843.1"
                     /translation="MLSFNWRAVSKVFNTGYSIPHSAETIHRRWFKRQTYVLALGSCL
                     VTLIGFPLYHLLTARGGETAFAGQYHRIAVLVVSLTSSLILIFSRHSRSRAELFSFTN
                     LLLLFICSAIDIALSEKPRRYTAFSLVPLFGSTYVFTSVRFMTVAYVSAAIVFTVLNS
                     WLHWIPEIIVIYNVAYFAAWWMALIRIRSLQRISYDQARLYERRIYDQRIKLARNLHD
                     SLGGDLMQLALQLSGNAPREQVLDLAQAVIAKTKNLVYTLEPTHENQHFPEYVTSYLH
                     RLRQIGRFTIDLRVDDDWPRLSIDKNLNLQAIFTEWMTNTLRHSMATDLNIYLYFRKR
                     HYFLVVRDNGTGFRWSGDRRGSGLRNISMRAGLLSAKVFARRTGTGTLFFLKGGNE"
     gene            complement(10945..11721)
                     /locus_tag="PLF85_03965"
     CDS             complement(10945..11721)
                     /locus_tag="PLF85_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE18844.1"
                     /translation="MVVAGFHAVTDLVEKSGFLSGDRLIVARQANDQRMQRLLAKAER
                     AQVTIIRKNYGELNQVAGTKNHQGIVLERQQALELKSFTKQDFVSAENGIYLALDGVQ
                     DPQNLGAIFRTALGLSVSGIFLPQKDSAPAGATALKASAGALTEIPLCFTGSIASLMQ
                     FISDKRPEIPLVALDASGELFSDGDTDLAGVQTPILLVVGSEQGLSRLALERATHRRR
                     LLMDSRLESYNVSVATALALYELVRRRSADLPDKRARSRT"
     gene            complement(11722..>12954)
                     /gene="cysS"
                     /locus_tag="PLF85_03970"
     CDS             complement(11722..>12954)
                     /gene="cysS"
                     /locus_tag="PLF85_03970"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNE18845.1"
                     /translation="TDVDDKTIKGATELMQKEPGKYADLKAALAAYTEPYSQAFFADL
                     KTLNVIPFDFHPRATDYIPAMLDLVERLIAKGVAYEQDGSVYYRISEKKDYGKLSRVD
                     LEQNKTGTRYNTDEYDKDDVRDFVLWKAESKDGVHWHSAHGDGRPGWHLECSAMIHEI
                     FKGGIDIHTGGVDLIFPHHENEIAQSESGYGDGFVKHWIHCEHLLVDNRKMSKSAGNF
                     YTLRDLLAQGKRPEVIRYFLLSAHYSQKVNFTAEALHQAEAGVEKIYSLWSRLERLQS
                     QQQGGDSVFLQVSADARENFTTCLADDLNTPRALAVVHEYLRAANAQLDQAKDQPASA
                     DVEQIRITLRHFDAVLALLDLMPKVGANAELQQLLEERNAARAAKDFKRADELRDQLL
                     KAGYKILDTKTGSHLEKV"
BASE COUNT         2781 a         3625 c         3604 g         2944 t
ORIGIN      
        1 acaaataatg ctgcggcgtg cgctcaagat ctttggcctg gcgctgctcg ccgcgttagg
       61 cggcatgctg gcgctgggct ggcaggcttt cggccgcacg gcagaaggcg aaagactcga
      121 acgcatgcgg cattcaccgc aatggctcgg taattcattt gaaaatccac agccgctgcg
      181 caatgatttc gccggctcga tctccgcgat gttcaagagc ggccgcttca cagctcccgt
      241 gggagcgatt ccggtcgaac gcacgaacgg ttcacagtac cacgcagctc ccacctcggg
      301 cctgagggtc acctggttcg gccattcatc taccctggtc gaaattgatg gcgctcgcgt
      361 gctgactgac ccggtcttca gtgaaagagt atccccctac ccgtggctcg gtccaaaacg
      421 ctggtaccca ccaccggtga ctttcggcga tctgcctgcg atcgacgtgg tcgtcatctc
      481 tcatgaccat tacgaccacc tggacatgta caccgtgaag ctgctgctcg caggcaaagc
      541 tatctttgtc gtgccgctcg gcatcggcgc acacctggcc tactggggta taccgaagga
      601 acggattgtt gaactcgact ggtggcagga aacgcaggtt ggccgactgc gcgttgtgct
      661 gactccggca cgccatgctt cggggcgctg gcttgtcgac ttcaacaaga aactctgggg
      721 cggtttcgcg ctcatcggga aaaagaaccg tgtctactac tcgggtgata cagggctgtt
      781 tccggcgatg aaagaaatcg gccggcggct cgggccgttc gacctcacga tgatcgaagt
      841 cggccagtat aaccgcagct ggcccgactg gcacatcggc cctgagcagg cggtcaaagc
      901 acaccgctgg gtaaacggcc gcaccatgct gccggtacac tggggcctgt ttgttctggc
      961 cgcgcacaac tggactgaac cgattgagcg tgtgctgcgc gaggcagaac ctcacggtgt
     1021 ccgcgtcgcc acgccaaaac cgggcgaagc gtttgaaccc gcgcaggcag cggccgatct
     1081 gaggttcgta aaacgctggt ggccggccgt cgaatggcag agtgccgcag agcacccgat
     1141 catctcaacg caggtcgagt aggcacttcg ttttctaata tcagtttcct gaaaagggaa
     1201 actgaaagca ggttccggaa accgcgcaag gctgcagaca ggccatttat ggccgttttg
     1261 cgagcgattc gcatcgccaa tcgcggcgtc tgcagccggc gcgggagttt ttccggaacc
     1321 tgctaaaaat taaaggtgaa gtattcgtcg caggtggcgc agggcaggtg cgccgggtac
     1381 tggccgcgaa agttggcgat aaaatcattc cgctggtttt gccagatttc ggcaagagta
     1441 cctttgtcca ggttgccccg ggcggagttc ttgttctggt caaccgtctg tttgcagtat
     1501 gcgatgtcgc cgttcgcgcg aataaacagg tcgcggcgca aatgccagca cgagtagcgc
     1561 tcgagcgggg tcatgtctga caccctgcgc tcgggcaaca ggcctgcata gcggttatat
     1621 ttttgcagca ggaagctcgc acccaggtct tcggcgagtg catagagcga atcgatttcg
     1681 gtttcgtttt cgacaacctt gaacgcctgc aggtagacaa gttcctgcgc attgcggccc
     1741 gcggcagtaa gcgcgcccgt taaactctgt acattcgctt tgacgcgatc aagcgtcacc
     1801 agcggcgcat cggaatagtc ggtgtatcgt tcgagcgaat tgagcatcac gatcacgcgc
     1861 agtttctggg cctgcgcatg cgtggccagc ggccataact tatcgaggtg caaaccgttg
     1921 gtctcgatga aaacataacg tacctgcggc tgttcaatca ggcgcgtgag atagccgacc
     1981 gcgtcgggat gctccagcgg ttcgccgagt cccgaagcgc agacagacgt gtctccataa
     2041 ccctggccga tatatgcgag caccttgtca aaatgcgcag cgctgagcat atgccttggc
     2101 tgctctatcg ccttgagtgg cgagaaatag ctcacatgct cggctgtcga aagaaactcg
     2161 agctcaagat aagagggaaa tgtaacaaga atttcaggat gcgtttcgat cgccggcttc
     2221 agcgcggtat atccctgtcc tgccggcagg tgctgcaaca aggcggcggt ctttgtcacg
     2281 gaacgctttg ttgccaggct gaagtcaagc cgcaggacac gcaggtcggg ctcttcataa
     2341 tgcacttcgg cgtgaaactg gttgatattc ttttcgacga acgggcgcag gccgactgta
     2401 gcgacgtcgg catcgctggc ctccatgatc tccagtgact cgagcaggtc gcggctgaca
     2461 atgtgcggag ctacaccggg cggcaggttt tccccgtaag tgatatcggc cctgtactcg
     2521 gtatggatac cgtgcagctt ttgcgtcagc tccgcgtcga gcagcgggaa aacgccgcca
     2581 cagatggcga tatggttcac cccgctttcg gcaaagagcg cccgaaccgc ggcaatctca
     2641 ctctgcacgg gcaacagctt tgcagcgggt aaaagccctt cgagtcgctg ccgcaggcta
     2701 tcgtctgcaa cacagaggtg aaccggtaaa cccagcgctt cgagtttcgc cgcgaaagct
     2761 tcgggcaggg cagccgcgcc ggactggtta aaatcaggcg tgtatgccgc agtcgtatgg
     2821 atgagtagga cggtctgcgt catgctgcag gactattcct ggtaatcttt caggtagatg
     2881 gtcacagcga ctttcttgcg ctgctctttc aaccagcggc cgtactcgat ctgttcgttc
     2941 tggctataga gtattgcgcg tacgttatca tacacctcgt cgaggccgat cgcacgcgca
     3001 ctttcgacgc gcacgcagta ataaccgccg tttgccccga caaagatctg cgaagtcgtg
     3061 cccatgggcg taccgtttac agcacccgcg agaatcgggt caatctgtgc gacctggtca
     3121 agccgcagat caaccattaa tccacccctc gccttactgc cgtgagcgga atattgcgtg
     3181 gcagctttcg caaaaccggc gctggatgag gcacccattg cgcgtgcagc cttcatctgg
     3241 ttgctgacct taagctcgtt gcccgcaacg ggaatgaata tcatgcgaaa cgtgtatttt
     3301 ttcccgacgc ggttcttgtt ctgccggtac cattcttcaa cctgcgaagg cgtcgggttt
     3361 gggacggaga cgcgcagctg catcacctgc tgcgtcttca gctggcgcga cagctcttct
     3421 ttgtaggtgt caaaatcaac ccccatgtcg cgcttgacaa ctttcttgaa atcgtcttcg
     3481 cgcagaatac cacgcatatc catttctttc ttgattgcgt tattgatacg ttcgggagaa
     3541 attgtaaccg attcttcgcg cgcaataacg tcgataatcg cgcggccaat caacgcatcg
     3601 agcgcatggc tttttatgct gcgcttgtct ttcagaggca tcttacgctt ctgcatgcgc
     3661 tccatctcga gcgacaggtc gatgctggta atcggcgcac tggccactac cgcgacgaca
     3721 tcggcgagaa ccgtagccgc aagcgaggca cacgaaaggg ctgcgctcgt caaaatgagc
     3781 aacaggagac gcgcggacat gttgaacctt cccggacttc gtatgcggcg taaaccgtta
     3841 tacgcttcgt cggctgcggg cagcttatga aatccattga cgtcccggtg ggggcagccc
     3901 gggtcagcca tgtcatccaa caccaaaaag cagcgcgtca tcgccatact gcaactttgc
     3961 ctggcggtgg gttttgccgg cttctggctg ctctatttcc tggaagatac ctgccgcgtc
     4021 caggggccca aactagccga ctgcctcgct tcgggtagca ccaccgaatg tctgaaaccc
     4081 gccctggctt gtgagcgcta cctcgcgttc gagaattcat tccctctgcc cgacctcggc
     4141 tatgtagccc cctgctgctc atttccgcat ggggcctgtg gcacaaccgc cgctggggtt
     4201 accttgctgg catcatggct ggttcagcac tggtattttt ggggctgctc gatgtttcgt
     4261 tcaacctgca gaatgctcgc tacagcatcg gactcgtcga cgcggcgatg aatgtgctga
     4321 tcaacgcggt ctgcctggtt ttcggccccc tgctcgtccg ctcctgctgg cagaaactcc
     4381 aaccgtagga cgtgggggag cttctatttc tgcttcaggt tcgagcggtc gaggcctttg
     4441 agccattcaa gcgtacgcgc aagaccttct cggtaagata ttttcggcgt ccaacccagc
     4501 tcgcgttccg cgcgctcaat ggaaaagcgc agccgtgacc caagattttt caccgtgtag
     4561 gtcgtcagca gcggccggct gcgcaggcgc agaattcgcc aggtgaattc catgagcaac
     4621 gctgcgccgt acgcgacaaa gtacggaaga tgtaggcgcg gcgccttagc accgagcgct
     4681 gcagcgattg ccgcgcagaa ctgctggaac gtcgtcaccg gcaggccgtg cgcctggtag
     4741 tagcgccacg caatttcttc cgcggcaatt ttcgtgtcgg cataaggctc accccatttt
     4801 ttgaggggag cttcttcggt gatgacccgg ctttcgtcga ggccgaatac gtcgttggtg
     4861 ctgagctcga caaaccgctg cacgcgcgcg ttcagggctg cgcggcagag tttttccatg
     4921 ccgtcgatat tgaccgcctg gaaaagcgag cgcggcgccc agtcagtcac aaccgccgcg
     4981 caatgaaaaa caatgtcgac gcccttgcag gccgcctcga gtgaagggcc atcgcgcagg
     5041 tcgccgtaaa caatttctac ccgctgcttt tttagcccga cttcaccggg gtcgccgcgc
     5101 atcaccagcg cacggacgcg gtatttcttt ttcaggcagg cgtcggcaag gtgcgagccg
     5161 ataaagcccg tcgagccggt gatcagtata atacgcatag gcgtccgcat tttttaattg
     5221 atgactggct ggtctatcgg tttacctgcg ttagtcaggg cacaatacac agtgtgctgc
     5281 tggatatcga taatagattg acatatatgg tatttcttgc catactacct gcgtgaaaac
     5341 gacgctgtac cttgatgacg acctgctggc acgtgctcag gaactgtccg gcatccggga
     5401 gaaaacccaa ctggttaacc ggggtctgca gaccctcgtc tcgcagctgg ccagccaaag
     5461 actggcacaa atgggtgggg ccttgtcctc actcaagacc ccaccacgcc ggcgcaagaa
     5521 tgcactctga atttattgcg gacacatcag tctgggtaaa ccatttcaag cggcgcggca
     5581 gctccgagtt cgtcagcgca ttgcgcgaaa atcgtgtgct gctgcacagc ctcgtttacg
     5641 cggaattggt cctcggtggc atagagaaga acgaggaggc tgatgaattg cttggcctgc
     5701 tgcggcgacc catcgaggcc acttacgacg agattcttga attcatccgc gtaaagaaac
     5761 tcgccggtag gggtgtcggc tgggtagatg ctgcgttgct ggcttcggcg gcactcagcg
     5821 gctgcgggct tctgaccgaa gataaggcac tcaggcgatt gacctgatag tggttagggg
     5881 ctgcgcgacc tgccgccagc ccgttacttc agatactgta acggatcttt tgggtcttta
     5941 cccactcgca cttcgaaatg caggtgcggc cccgtcgctt tgccggtcct acccacgtaa
     6001 ccgatgagct cccccgcagc aacacgctga cccttacggg ctttcgtgcg tgactggtgg
     6061 cagtagtacg tgaagagatt ttctgagtgt ttcacgatga tgtaattgcc ccaccacttg
     6121 ttggcggtgt tctcgtagat ttcacctgct tttgccgact tgatgggttc gccgatcggc
     6181 gccatgatgt cgataccgcg gtggaagtcg ccgctcttct tggttacggg atcaatacgc
     6241 gggccaaact ttgaattcac acgaccatcg accggcatcg caaaacctcc cgcaccgacg
     6301 ctgggcggtt gcggcgccgg tttctgtgca acagggcgca ggtaaccgtt aaacaaccag
     6361 ccactcttgc ccgcgtagtt tgcctgcgcc cagaaatcgc tgagcccatc gatcgtgtct
     6421 ttcgaagccg aataggcgat cacctcaacc tcggcttcgg ccggcacgag ctgtatgaac
     6481 tcccccgcaa gcgaaggttc actgcgcata cgcaggcccg atgaactgat gttgtacttc
     6541 gcttctttct tcttggtgct cggttgcacc tgctcgggca cgttctttag gtacatctgc
     6601 ggaatataac cttcgctctc agtcgccgtg cggataagcg cccacccttc gacttcgctc
     6661 agggaccggt tgctgagcgg agtcgaagca cgggggttca caaccataac gacctcgacc
     6721 ttgtcgcccg cacgcacgcg gtcgagattc tgtgagtcgg aatcggaatg ccaggtcttg
     6781 aggcgatagt cctgcgtgac gatcgcaaaa actttcggcg cttcgggaat tcccgtcagc
     6841 tcgttgtggt caatgagccg catcttgccg gcatttttcg tatcgaccgg cagcgagata
     6901 tccaggccgg gtttgagttt ttcgaggtca gacctgaact ccgcgaagcg cgccagaatt
     6961 ttttcgacct tcttctggct ttcgctgcgc actgtatcgg tcttctgctg cgcctgtttg
     7021 cgcaggtctt cgacctcgag ggcaaagacc gccgacaatg tgacaataat cgtgagggat
     7081 agaactccgc gcagcatgcc gttagagtcg gcgcgcaaca gtcggggtca aatagaaatg
     7141 cggataccaa tctgagagct tgtgcggcag gatacttatg cgcactgccg caccagccat
     7201 cggccgtacg gggttttgtc caaagttttg acaaaactta atggccttgc gacggctatt
     7261 ttgctatact tttcgcatga cgcaagcagc cgcggccatg gcagcctctc tcgtagacgt
     7321 gcagtccagc gccgacaccc gccgaatcgc tattgataag gtgggtatca agggtattcg
     7381 ttacccgatt caaattcgtg acaaaacggg cgaaacccag aacactgtgg cggagctcaa
     7441 tatgtacgtt tacctgcccc agcagttcaa gggcacgcac atgtcgcgtt ttgttgaaat
     7501 tctcgagcgc aagaaacacc tcgatgtgcc aaccttcaag gcgctgctgc ccgaaatgct
     7561 cgaacggctc gaatcgcacg tgggtcaaat cgagatgaag ttcacctact tcgtcaacaa
     7621 ggcggcgccc gtgagcggag tgcagagcct gatggattac gacgtgactt tcatcggcca
     7681 ctgtgaaaac ggcataaccg acctgcgtat caaggtggtg gtacccgtga ccagcctctg
     7741 cccctgctcg aaagaaattt cacaatacgg cgcgcataac cagcgcagcc acatcaccat
     7801 cacagcccgg ctgagcgaac tcgccgaagg cgccaatact ctttggattg aagatatcat
     7861 tcgcctggct gaagaaagcg cctcgtgcga actctacggg ctgctgaagc gcccggacga
     7921 gaagtacgtc actgaaaagg catacgaaaa cccaaaattc gttgaggata tcgtgcgcga
     7981 tgttgcgcac cggctcaacc aggacgaccg tatcagccac tacgttgccg aagccgaaaa
     8041 cttcgagtcg atccacaacc actcagcata cgcgctgatt gaaaagcaca agtgattggt
     8101 gctttcgcac caaccacttg tgcctaagtg gcgcaggcaa taaaaaaccc cgccagagcg
     8161 gggtcacttg gtaaaaatca ggcagtgccg gttagaactt atagcctata gttacgctcg
     8221 gtacgatgtt gtggaacatt ttgtcatcaa agatggcata gtaacgcaca cccacatcag
     8281 cagatacgcc atcaaagtcg aacccgtacg ctacgcttgc tgaggcagag aaagatgaac
     8341 ccttaacctt tgtcgtagta gtttcagaac ccgttacttc tgttttcgtt tcgcctgtac
     8401 ccagcgaaat accagcgcca acaccgagct tcagtccacc tatgatgtac tgagcctgtg
     8461 cgtgaatcgg tgcgaacgcg atatttgacg taattttagc agtggtccct gcttcagagt
     8521 ttgtccagtc cagtttgtaa atttgtagat aagcaagacc cagaccgtat ttgaaatcac
     8581 cgccaagcat cagatccgca ccagcggcga taccgccttt ggtctgggtt ggtgattttg
     8641 atgtcgaaaa accggcagat gtttctgagt ccttgatagt tttttcgagg tcagaaaaga
     8701 aagtgcttga agcgaaatta tatccacccc aaacagcgac cgacatcttg ctttcgaccg
     8761 atgccgcagc agaggtcaat ttttcgttcg ccttgttctg cacatccgca gcttttgcat
     8821 tcgctgcatc ctgtgctgct tttgcttttg catcggcgtc agcttttgct gcatctactt
     8881 tggccgcagc cttagctgcc ggctttgcct ttttttgcgc gtgtactgca ccgatcaggc
     8941 tgagtactgc gagcattgcg agaatttttt tcatgctatc tcctggaaaa gaatttagcc
     9001 gcaccgccga aaaaagaccg gcaacctgcg ctgccggttt ttcagagata caacgctcta
     9061 cactaccata cggagataga gataggtttg tcaatgggca ggaaatccct taataggaat
     9121 atctgccgag ggaagattat tcgaggcgaa tgatgccggc gcgcaccgct tgcgccacag
     9181 cctggccgcg gctggtcaca ttcagtttct tgtagatttt ttcgatgtgg tggcgcactg
     9241 tgtgcacact gatatcaagt tcttcggcga tatcttcgta gcgcaggccc aacgaaataa
     9301 gattgagaac ctgaagctcg cgcttgctga gcgacgattc ggtcgccttg tgcggggcaa
     9361 agtgtctgag aattttctct gccacacgcg gcgtcagcgt cgaaccgccc tgcgcaataa
     9421 cctcgagttc tgacaagaaa cgtgcagggg gagtatcttt catgatatag ccgtttgccc
     9481 cggccatcat gcaggcaata atctcgttct cgttttcgta tgcagtgtag acgaccacct
     9541 ttgcttcagg gtatttttcg cgcaggccgc ttatggcttc ggtaccacgc acgtctggca
     9601 gtttaagatc gagcacgatc aggtcaggat gcagggcacg gctatcggcg agtgcggcct
     9661 gcaggtcacc gtaagtacct gaaacaccga actccttatt cttcttaaag agatctgcca
     9721 gcccctgccg gactgttttg ttatcttcta ccacaagcaa gtttttcatt cattaccacc
     9781 ttttaagaaa aacaatgtgc cggtaccggt tctgcgcgca aagacttttg cgctcaacaa
     9841 cccggcccgc atggaaatat tcctgagacc cgaaccgcgc cggtcgccgc tccagcgaaa
     9901 gcccgtaccg ttatccctga ctaccagaaa gtagtgtcgt tttcgaaaat acaagtaaat
     9961 attgagatcc gtggccatcg aatgccgcag cgtgttggtc atccactcgg tgaaaattgc
    10021 ctgaaggttg agattcttgt cgatcgagag ccttggccag tcatcatcga cgcgcaggtc
    10081 tatggtgaac cgacctatct gcctcaggcg gtgcaggtac gaagtcacgt attcgggaaa
    10141 atgctggttt tcatgagtcg gctcaagggt ataaacaagg ttcttggttt tcgcaatgac
    10201 tgcctgcgcc aggtcgagta cctgctctcg cggcgcgttg ccgctcagct gcagcgcaag
    10261 ctgcatcagg tcaccgccga gcgagtcatg tagattacgg gccagcttga tgcgctggtc
    10321 atagatacgc ctttcataca acctcgcctg gtcatagctg attcgctgca ggctgcgtat
    10381 gcgtatcagc gccatccacc aggcggcaaa gtacgcaaca ttgtagatga cgatgatttc
    10441 gggaatccag tgtaaccagc tgttgagtac ggtaaaaact attgcggcag acacgtaggc
    10501 cacggtcata aaacgcacgc tcgtgaaaac ataggtactg ccgaaaagtg gtacgaggct
    10561 gaaagctgta tagcgacgcg gtttttcgct gagcgcgata tcaatagccg aacagataaa
    10621 cagcagcagc aggttggtga aagaaaaaag ctccgctcgt gaacgcgagt ggcggctaaa
    10681 gatgagtatc aatgagctgg tcagcgagac gaccaggacg gcgatccggt ggtattggcc
    10741 ggcaaaggct gtttcgcccc cacgcgccgt gagcaggtga tacagcggaa aaccgatgag
    10801 cgttacaagg cacgagccca gtgccagtac ataggtttgc cgtttgaacc agcgccggtg
    10861 aatcgtctcg gcgctgtgcg gtatgctgta gccggtgtta aataccttgc tgacggcacg
    10921 ccaattgaag ctgagcattt tgtcctatgt gcggcttctg gctcgtttat cgggcagatc
    10981 agcagaacgc ctgcgcacta actcgtaaag cgccagcgcg gtggcaacag aaacgttgta
    11041 actctcaaga cgactatcca tcagcaggcg acggcggtgc gtcgcccttt cgagtgcgag
    11101 gcgactcaaa ccctgctcag aaccgacaac cagaagaatg ggagtttgca cccctgcgag
    11161 gtctgtgtca ccgtcagaaa agagctcccc cgaagcatcg agggccacca acggtatttc
    11221 aggccgcttg tcagaaatga actgcatgag cgaagcgata ctgccggtga aacacagggg
    11281 gatctcagtg agagcccctg cagatgcctt gagtgcggtt gcccctgcag gggcgctgtc
    11341 tttctgcggc agaaaaattc cgctgaccga aaggccgagc gccgtccgga aaatagcccc
    11401 gaggttctgt ggatcctgaa caccatcgag cgcgaggtag atgccgttct cagcggaaac
    11461 gaaatcctgt ttagtaaaag atttcagctc aagagcctgc tgtcgctcga gcacaatacc
    11521 ctggtggttc ttggtgcccg caacctggtt cagctcgcca tagtttttgc ggatgatagt
    11581 tacctgcgcg cgctcggctt ttgccagcag ccgctgcatg cgctggtcgt ttgcctggcg
    11641 cgcgacaatc aaccggtcgc cggaaagaaa tcccgatttt tcgaccaggt cagtaacggc
    11701 gtgaaagcca gcgacgacca ctcagacctt ttccaggtga gaacccgtct tcgtgtcgag
    11761 tatcttgtaa cctgccttca gtagctggtc gcgcagctcg tccgcgcgct tgaaatcttt
    11821 cgctgcgcgc gccgcgttgc gctcctcgag taattgctgc aattcggcat ttgcgccgac
    11881 tttcggcatc aggtcaagca gcgccaaaac tgcgtcaaag tgccgcagcg taatacgaat
    11941 ttgttctaca tcagcagatg cgggctggtc ttttgcctgg tcgagctgcg cattggctgc
    12001 ccgcaggtat tcgtgcacaa ccgccagcgc gcgcggggta ttcaggtcgt ctgcaaggca
    12061 ggtagtaaaa ttttcgcgcg cgtcggcaga gacctgaaga aaaacagaat ctccaccttg
    12121 ctgctgtgac tgcaaccttt cgagccgcga ccagagactg tagattttct ccacgcccgc
    12181 ttcggcctgg tgcagggcct ctgcagtgaa gttcactttc tggctgtagt gtgcagagag
    12241 caggaaataa cgaattacct cggggcgctt gccctgcgcg agcaggtcgc gcagagtgta
    12301 aaagttaccg gcggattttg acatcttgcg gttatcgacc agcaaatgct cgcagtggat
    12361 ccagtgtttg acgaaaccat cgccgtaacc cgactcgctc tgcgctattt cgttttcgtg
    12421 gtgaggaaag atcaggtcga caccacccgt atggatatcg atgccacctt tgaatatttc
    12481 gtggatcatc gctgagcatt cgaggtgcca gccaggccgg ccgtcgccgt gggcggaatg
    12541 ccagtgaacc ccgtcttttg actcggcttt ccataacaca aagtcgcgca cgtcgtcttt
    12601 gtcgtattcg tcggtattgt aacgcgtgcc tgtcttgttc tgctcgagat caacgcggga
    12661 gagtttaccg taatctttct tctcactgat gcggtaatag accgaaccat cctgctcgta
    12721 agcaacgcct ttggcaatca gccgctcaac caggtcgagc attgcgggaa tatagtctgt
    12781 cgcacgtggg tgaaaatcaa aaggaataac gttcagggtt ttcaggtcag caaagaatgc
    12841 ctggctgtaa ggttcggtat atgcggcgag cgcagccttc aggtcggcgt atttgccagg
    12901 ctctttttgc atgagctcgg tcgcaccctt gatcgtcttg tcatcgacgt cggt
//
LOCUS       DAOQJF010000046        12743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2421_length_12743_cov_4.765290, whole genome shotgun sequence.
ACCESSION   DAOQJF010000046 DAOQJF010000000
VERSION     DAOQJF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2421_length_12743_cov_4.765290"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLF85_03975"
     CDS             complement(<1..768)
                     /locus_tag="PLF85_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18846.1"
                     /translation="MIDAKQNRSASPFALRYKLALLLLVILFIVLAYRAVELAIASPL
                     PPSVIEQAQKVRRGVVFDSRGHELAISRDTVSVGVKPLEARQQTVGWDIVARVTGTPK
                     NELLQRIQASEKFFYLRRKMPPDEAAPLRKLDLAGLVLQNEPDRYYPNGRLGSQIVGF
                     TGLDGEGLSGVEFSQNQTLSSTRKGAHDFIGQNIHLSINSFVQHQLEKTLAQGLEDSV
                     SRHAMGVIIETQTGRVLAMSSQPDFDPNAPNKSPEATW"
     gene            complement(774..1274)
                     /locus_tag="PLF85_03980"
     CDS             complement(774..1274)
                     /locus_tag="PLF85_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18847.1"
                     /translation="MSRRLLQVYLVVAASLGLAWLLSRIARDVLIDFARVTNESMLPH
                     LSPGQRLVISRLSPCLHLPFMQRGVACSPCAPGNAYVFRYPGRSEQRLVKFAVSKEEF
                     DSGSARISSGDIISFTPRTPALSNPAETGRLTCYFVGSNGERSIDSRDFGPVPADEVE
                     GRVVYP"
     gene            complement(1271..1828)
                     /locus_tag="PLF85_03985"
     CDS             complement(1271..1828)
                     /locus_tag="PLF85_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18848.1"
                     /translation="MKLTRTHSRAIRIITIAALLLLAVSAGVLLREYLMLKKIQATLR
                     QSPSQIKNEPEQNTPAEAVPEAPLVSAVSRSADGRYIRARANFSVFKKKVEAHPEILR
                     QWGRVGWKIVGDVPALCVRSVGETAPFRPLGVKDGDCITHIDGESINQPMRNMGIWLT
                     LPARSRLKIDTLRDGKRISYELVRK"
     gene            1888..2142
                     /locus_tag="PLF85_03990"
     CDS             1888..2142
                     /locus_tag="PLF85_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18849.1"
                     /translation="MRKSGLRLLVPVFSAAALVVLSATGCGKSECKRYNEKFCADANS
                     EACKQAKERSKDWSADKCRIERNQLEIEEQSKKLDKELSE"
     gene            2142..3299
                     /locus_tag="PLF85_03995"
     CDS             2142..3299
                     /locus_tag="PLF85_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE18850.1"
                     /translation="MRDILASRIHGIDTSEIRKVFDLAAKIKNPLNLSIGQPDFPVPS
                     PVKEAMVKALTDNKTGYTPTAGLVELREAIAGYLEPKTKFRYEADNVIVSTGVASILF
                     LLFQAIVDKDDAVLLLDPYFLIYPALVKYHEARQYTVPEDFTTAHIEQLKEQLKRDNK
                     RLKLIIFASPSNPTGKILSREQLTLLSQLADEQDAVIASDEIYAAFDYEAKHVSMASL
                     NPARTLTLNGFSKSHAMTGLRVGYLAAPPQYAGIVQKMVTLQQYTMVCAPHAVQWGAI
                     TALKTGIEEELKIMRRRRDLVVNKLKTVTTLPVPDGAFYVYPAIPIDSADFVTQAIER
                     RLLLVPGYIFSANRKSIRISYATREEVLEEGCDIFCQMVTSMKETMASEAI"
     gene            3302..3871
                     /locus_tag="PLF85_04000"
     CDS             3302..3871
                     /locus_tag="PLF85_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18851.1"
                     /translation="MKNTGKSLLRFLAIGWLSLQPVLAQDYDDDGMETVTTSAVPKAP
                     DCNRFPFGKGCLAFGFGPLITSDGKDLIYGAGAGLSYFMLDRLALAANGGAVFGNGFQ
                     DYSIGPALTYYIGPFGGYLITPSLSATRHFLRGNISAEGWAYGPSVGLMTRFFGRAYW
                     GISVGYFTYEVAGYKSSDWSWSPVVFVPF"
     gene            complement(3928..4668)
                     /locus_tag="PLF85_04005"
     CDS             complement(3928..4668)
                     /locus_tag="PLF85_04005"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNE18852.1"
                     /translation="MKETIAAAARLLHHARHPIFLSGAGISAESGIPTFRGQDGLWNN
                     YRAEELATPEAFARDPHLVWQWYNWRKNLIAECKPNPAHYAAAELQQLLPQLLCVTQN
                     VDGFHALAGLSQILEMHGNIYRTRCLSCKTVAENKNEITPTTPCPACGKIPLRPDVVW
                     FGEALPRAVMDPLHERLQVADAILVVGTSGTVYPAAGFAVEVRRRRGAVIEINIDTAH
                     RPYADDIYLHGKAGDLLPAIVAELRQLT"
     gene            4790..5164
                     /locus_tag="PLF85_04010"
     CDS             4790..5164
                     /locus_tag="PLF85_04010"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNE18853.1"
                     /translation="MLAITLLALLHIVFFVFESLLWTTPTVRKIFRQSEADAQTTRML
                     ALNQGYYNLFLALAAIAGVVLMYRVDVPIAVSVAVLTVSLGSMVGAALVLLFSAGKKM
                     IRGVLLQGLAPLVCLVLIWRPV"
     gene            complement(5202..5591)
                     /locus_tag="PLF85_04015"
     CDS             complement(5202..5591)
                     /locus_tag="PLF85_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18854.1"
                     /translation="MANAEYGLTNEIGIGGSIGYWGYSESLSTFGATVKYSYTIIPII
                     VSGAYHIPIKNPKLDLAVGISLGYYMVSSSVESSISGFSYASATGSGVAWGVFGLARY
                     FVSDSIALRGKLGYGITLLEVGVDFRF"
     gene            5932..6360
                     /locus_tag="PLF85_04020"
     CDS             5932..6360
                     /locus_tag="PLF85_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18855.1"
                     /translation="MQVNKSESKWLVVIEDQDNKLGLINMIRHLDKVVSELEMAKIPN
                     LLLEIDLAKLNSANSELIAKFVELQSILVRTDGRLNVINANPELKSSFDVVMLDKIIP
                     IQYVGQEDPGEDGYAYDDYELPDDDDDDEDDEEEDGDEDE"
     gene            6393..7022
                     /locus_tag="PLF85_04025"
     CDS             6393..7022
                     /locus_tag="PLF85_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNE18856.1"
                     /translation="MFDWDGTLYRSIDLIEASIVAAGQSVGRHISSAVARHIIGLGLK
                     AAQSILFPGEQPDEKFLREFHLAYRRHYEAQEKDLSLYPGAYELVRDLADQGARIAVA
                     TAKSRSGINSAMAATGLDRYVSWSRTPEECRPKPDPQMIDEIVLEARAEKARTLMVGD
                     TTHDLAMGLNAGVHIAGLCHGAHTEPELSNLNPLVLCNDIAALRRALLS"
     gene            complement(7028..7921)
                     /locus_tag="PLF85_04030"
     CDS             complement(7028..7921)
                     /locus_tag="PLF85_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE18857.1"
                     /translation="MKVTGFSFIRNAIRYDYPIRESLLSLLPLVDEIVVAVGKSDDDT
                     LELVKNIDPARIRIIETEWDDSLREGGRVLALETQKAYEQIDGGDWAFYIQGDEVLHE
                     KDYPAIRSAMQKWANDPRTDGLLFDYRHFYGSYDYTGASRRWYRREVRVVRPPHFTLN
                     PQMRIFSYRDAQGFRKNGNEKLRVRQVNAGIYHYGWVKPPAAQMQKQQTFNKYWHDDN
                     WMQKNIPSAAEFDYSNIDSLTRFTGAHPAVMHERIARKNWQFDFDISRSRFTFKEKVS
                     GLVERLTGYRPGEYKNYRKIG"
     gene            complement(7971..8432)
                     /locus_tag="PLF85_04035"
     CDS             complement(7971..8432)
                     /locus_tag="PLF85_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18858.1"
                     /translation="MKKLLSMGHALIFTLAMALTAGISADSPCKGLSESECSSNDKCT
                     WVSGYSQKDGDKVKAYCRAKPGQGDSDKSEKKTKKKKEKSDEAVKADGDDDKAEKKDK
                     KKKSKKKKDKEDKAEAKEDKKDDDSKSEKKEKKAKKDKKESKKKKKSKKDE"
     gene            complement(8497..10134)
                     /locus_tag="PLF85_04040"
     CDS             complement(8497..10134)
                     /locus_tag="PLF85_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18859.1"
                     /translation="MAVAEVDNDLGYYPTSMPARNSIRNLLLLALVTTTSLQAALPFT
                     GPADIAAADQVPAGERALRLADTLLLEPGYRRARNLAAEWHRYVSGFVADEKQAQQIE
                     TLANERAILYFLRETEGSLAPAFAKEAERVLNQFGNGALVAWRLRSLAGKVVLAHNAE
                     RLKDLDTSETRREIGARYLTVPISGAGFNFSLDAEWDVTRLPNYPLTEANSEFLFIQT
                     DNELRLTGRAEYRQLPDSAYRFISGDKFTALPYLVQNGMRILPVSAGDMRLFICYPFA
                     GYLFYALRGTVALLLLVSLVIAVRGIRSLRATVKDVLENRQGRWLGEHYEKSLSLNEQ
                     ALKVTDRSVALVSEIKDRDAALISDLGRKLGEITSGIEEQTRFLIEEAVSRQKPAPAT
                     AAVPTGVQRPLHRKPAPKAPIIIDGSAPGDVQVSIELDLPLTDEKQLSPHAKAEYVSS
                     LRRRAMEKTGQREYVHDEKIDNYDFTPEDPVALPQKPAFEPREIPDSSDLEYVQKFRY
                     SGKPRVLPLEVKPANSAGLRMREDLHQQELVVTGEEE"
     gene            10226..10633
                     /locus_tag="PLF85_04045"
     CDS             10226..10633
                     /locus_tag="PLF85_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE18860.1"
                     /translation="MAVSTHFPHGDKVLLADVDEGDALQLASFLELNGYTVRRSTEIT
                     EALTYFSDFSPGVVVIDSSLPAKSWLTLIQAIRSYEVEKDTDYRSIILVTAGRFTNDL
                     QQKAIKAGAHEVIGRPVDGATLLKRIAAHTGVY"
     gene            10637..11674
                     /locus_tag="PLF85_04050"
     CDS             10637..11674
                     /locus_tag="PLF85_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18861.1"
                     /translation="MFARLLRQRALQFLLLFVGAYPVTAQRTAVAGKRSAPNPLGVYL
                     QLAESLGIDHTHRRIEWDYTLDSYYTSGGMIINFTEKPIPNLGEESEAKVYQHLLTKP
                     FVPRYAVLEASVNPMPVAGVVIRENANDVYQRAQVGGESFNLIRSATRGFQEPYAVSL
                     FLGNVVRYRPLARTPEEREQNKASGYQSSLGYSGYLVSYGTHHIKDNELFQDHWVELE
                     LKVKGDQLFTLQKLRWDYKIGVKLHDNPFISDVAVIGIRRNRLDYTADAWSFLANSGF
                     EYRIDFRLANLKPVRQYFEVNKKWPVKTAAAFSLALGFTWESESLYSGPLDNYPGGEH
                     FQILLRPNIEF"
     gene            11733..12527
                     /locus_tag="PLF85_04055"
     CDS             11733..12527
                     /locus_tag="PLF85_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNE18862.1"
                     /translation="MNFEKFNALVEGRVNYGELENAAVVSEYKNIGCGDGYRLYLDIE
                     GDQIRDARYTTTGCSFSLASLAIICDLAKGKTLDAALQIAPEELEPAIEGYPERRKNY
                     AVTAVQALQKAIRDFREQSGLKGEQVISRDKTIAHLKEHGHLRGLNLNSVMLDKLDLS
                     GVDFSGSNLQHAFLRESNLTGANFAGANLRGAFLNDCVLDKTNFAGADLRFAKLLGAK
                     ISDCIFDGALYDIGTRVDTKNMHIFEVMTQKGKELYLKGGAQAVTA"
     gene            12571..12643
                     /locus_tag="PLF85_04060"
     tRNA            12571..12643
                     /locus_tag="PLF85_04060"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12604..12606,aa:Met,seq:cat)
BASE COUNT         2664 a         3504 c         3636 g         2939 t
ORIGIN      
        1 ccaggtggct tcgggcgact tgttgggagc gttggggtca aagtccggct gcgacgacat
       61 ggcgagcacg cgtcccgtct gcgtctcgat gatgactccc atggcgtgcc ttgacacgct
      121 gtcttccaaa ccctgtgcca gtgtcttttc gagctggtgc tgcacgaacg aattgatact
      181 gaggtgaata ttctgaccga tgaagtcatg cgcgcctttg cgcgtgctgc tcagtgtctg
      241 gttctggctg aactccaccc ccgacaaacc ttcgccgtcg agaccggtaa aaccgacaat
      301 ctgcgaaccg agacgaccgt tagggtagta gcggtcgggt tcgttctgca agacgaggcc
      361 cgccaggtcg agcttacgca ggggagctgc ttcgtccggc ggcattttgc ggcgaagata
      421 aaaaaacttt tcactcgcct gtatgcgctg cagcagttca ttttttggag tgccggttac
      481 gcgggcaaca atgtcccagc cgacggtctg ctgtcttgcc tcgagaggtt taaccccgac
      541 cgacaccgtg tcgcgcgaga tggcgagttc gtgcccgcgt gagtcgaaca caacaccgcg
      601 cctgactttc tgtgcctgct caatgactga aggaggcagt ggtgaagcaa tcgcgagttc
      661 aactgcgcgg taggcgagca cgataaaaag tatgacgagt aacagcagcg ccagcttata
      721 gcgcagtgca aacggtgaag ccgagcggtt ctgtttggcg tcgatcatct gtcttagggg
      781 tagaccaccc tgccttctac ctcgtctgcg ggcacgggcc cgaagtcgcg tgaatctatg
      841 gaacgttcac cgttgctgcc tacaaagtag caggtcaacc gccccgtttc cgccggatta
      901 ctgagcgccg gcgttcgggg cgtaaaggag attatgtctc ctgatgagat acgtgcgctg
      961 cctgaatcga attcttcttt agagacggcg aacttcacga gccgctgctc actgcgcccc
     1021 ggataacgaa aaacgtatgc attgcccggg gcgcaggggc tgcaggccac accgcgctgc
     1081 ataaagggca ggtgcaggca gggtgacagg cggctgatca ccagccgctg tccgggcgaa
     1141 aggtggggga gcatcgattc atttgtgacc cgcgcgaaat cgatcaagac gtcgcgcgcg
     1201 atgcggctaa gaagccaggc caggccgagc gatgcggcga ccaccaggta aacctgaagc
     1261 aagcgcctgc tcatttgcgc acaagttcgt acgagatacg ttttccgtcg cgcagcgtat
     1321 cgattttcag cctgctgcgc gcgggcagcg tgagccagat acccatgttg cgcatgggct
     1381 ggttaatgct ttcgccgtcg atatgcgtga tgcagtcgcc gtcctttacc ccaagcgggc
     1441 ggaaaggtgc tgtttcgccg acactgcgta cacagagcgc aggcacatcg ccgacgattt
     1501 tccagcctac gcggccccac tggcgtaaga tttccggatg cgcttcgact ttcttcttga
     1561 atacggaaaa attagcgcgg gcgcggatat atctgccatc ggccgagcgg cttaccgctg
     1621 aaaccagtgg tgcctcaggt acagcttcgg ccggtgtatt ctgctccggt tcgtttttta
     1681 tctgcgatgg cgactgccgc agtgttgcct ggattttttt cagcatcagg tattcccgca
     1741 gcagtacgcc cgcggaaact gccagcaaga gcagggcagc gattgtgatg atcctgattg
     1801 cccggctgtg cgttcttgtt agcttcatcg atcctgatag ccccggcttt tacttgacgc
     1861 agcaatcaaa ttccctgtga ggcagtcatg cgaaaaagtg gattgcggtt gctggtacct
     1921 gttttctcag ctgccgcatt ggtcgttctg tcagccacgg gctgcggcaa atcggagtgc
     1981 aaacgctaca atgaaaagtt ctgtgctgac gccaatagcg aagcgtgcaa gcaggcaaag
     2041 gagcgcagca aagactggtc ggcagataaa tgccgtatcg agcgcaacca gcttgaaatt
     2101 gaagagcagt cgaagaaact cgacaaagag ctttcagaat aatgcgcgat attcttgcct
     2161 cgcgcatcca cggtattgat accagcgaga tccgtaaggt ctttgacctt gcggccaaaa
     2221 tcaaaaatcc gctgaatctt tcgatcggcc agcccgactt ccccgtgcca tcgcccgtga
     2281 aagaggcgat ggtaaaggca ctgaccgaca acaaaaccgg ttatacgccg acggcgggcc
     2341 tggttgagct gcgtgaagca atcgccggtt acctcgagcc aaagacaaag ttccgttacg
     2401 aggcagataa cgtgattgtc tcgaccggcg tggcatccat cttgttcctg cttttccagg
     2461 cgatcgtcga caaagatgac gccgtgctgt tgctcgaccc gtacttcctg atctacccgg
     2521 cgctggtaaa gtaccatgaa gcgcggcagt acacggtacc tgaagatttt accactgcac
     2581 acattgagca gctcaaagag cagctgaaac gcgacaacaa gcgccttaag ctgattatct
     2641 ttgcctcgcc gtctaaccct acaggaaaaa tcctgagccg cgaacagctt accctgctgt
     2701 cgcagttagc cgacgagcag gatgctgtca ttgcctccga tgaaatttat gcggcattcg
     2761 actacgaagc gaagcatgtc agcatggctt cactcaaccc cgcgcgcaca ttgacgctta
     2821 acggcttttc gaagagccac gccatgacgg gtttgcgcgt cggttacctc gccgctccgc
     2881 cgcagtatgc tggtatcgtt cagaagatgg tgacgttgca gcagtacacc atggtctgcg
     2941 cgccgcacgc ggtgcagtgg ggcgcgatca cggcgctaaa gaccggcatc gaagaagaac
     3001 tgaagatcat gcgccggcgc cgtgacctcg tcgtgaacaa gctgaagacg gtcacaacgc
     3061 tgcctgtacc cgacggcgcc ttctatgtgt acccggcaat accgatcgat tctgcagatt
     3121 ttgtgacgca ggcaattgaa cgccgtctgc tgctcgtgcc gggttatatc ttcagcgcca
     3181 accgcaaatc gatacgcatc agctatgcga cgcgcgaaga agtgctcgaa gaggggtgcg
     3241 atattttctg ccagatggtg acttctatga aagagaccat ggcgtccgaa gcaatctgaa
     3301 gatgaaaaat actggtaaga gtttattgcg ttttctggcg atcgggtggc tctcactgca
     3361 accggtgctg gcgcaggatt acgacgatga cggcatggaa accgtcacaa cgagtgcggt
     3421 acccaaagcg ccggattgca accgctttcc cttcggcaaa ggttgtctag cgtttggttt
     3481 tggcccgctc attaccagcg atggtaaaga cctgatctat ggcgcgggtg caggcttgag
     3541 ctacttcatg ctcgatcgcc tggcgctggc ggccaacggt ggtgctgtct tcggcaatgg
     3601 tttccaggat tactccatcg ggccggcgct gacctactat atcggccctt tcggcggtta
     3661 tctcattacg ccgtcgctga gcgcgacgcg gcattttctg cgcggtaata tcagcgccga
     3721 aggctgggca tatggtcctt cggttggcct gatgacccgc ttcttcggcc gcgcctactg
     3781 gggtatctcg gtgggctact tcacttatga agtggccggt tacaagagca gcgactggag
     3841 ctggtcaccg gtcgtttttg ttcctttcta agaggctcct gaaaaggagc cggcgcggga
     3901 gtttttccgg aacttgctaa caggctacta agtaagctgc cgcaattccg cgacaattgc
     3961 cggtagaaga tcgcccgctt tgccatgcag atagatgtcg tctgcatagg gcctgtgcgc
     4021 ggtatcgatg ttgatttcga tcactgcgcc gcgcctgcgc cgaacctcga ccgcaaaacc
     4081 cgccgcagga tagaccgtcc ccgatgtgcc tacaaccaga attgcatcgg cgacctgcag
     4141 ccgttcgtgc agcggatcca tgacggcgcg cggcagtgct tcgccaaacc atacgacgtc
     4201 ggggcgcagc ggtatcttac cgcaggccgg gcagggtgta gtgggagtaa tctcgttttt
     4261 gttttcggca acggttttgc agctcaggca gcgcgtacgg tagatattgc cgtgcatttc
     4321 aagaatttgg ctgagccccg ccagagcgtg aaagccatcg acattctgtg ttacgcagag
     4381 caattgtggc agcagctgct gcagttccgc agccgcgtaa tgcgccgggt tcggtttaca
     4441 ttcggcaatc aggttcttgc gccagttgta ccactgccag accaggtgcg gatcgcgcgc
     4501 aaatgcctcg ggagtcgcca gctcttcggc acggtagttg ttccacaatc cgtcctggcc
     4561 gcgaaatgtg ggtataccac tctcggccga aatgcctgcc ccactgagga atatcgggtg
     4621 ccgggcatga tggagtaggc gggccgctgc tgcgattgtt tctttcattt cgctcctaca
     4681 catcctgagc cgttgcgcgc ccggcaagca ggctttcaat gaccgtttac cctgagcgct
     4741 gcacggttct tttggtttga cagccgcgct gcatcatgtt ggcttagtca tgctagccat
     4801 taccctactg gcgctgctac acattgtgtt ctttgtcttc gagagcctgc tgtggacgac
     4861 gccgactgtc agaaagatct ttcgccagtc agaagccgac gcgcagacca cccgcatgct
     4921 ggcgctgaac caggggtact ataacctttt tctggcactc gcggcgattg cgggtgtcgt
     4981 tttgatgtac cgtgtcgatg ttcccatcgc ggtgagcgtg gcagtgttga ccgtcagcct
     5041 cggcagcatg gtgggagctg ccctggttct gctgttttct gccggaaaaa aaatgatccg
     5101 cggcgtattg ctgcaggggc tagccccgct ggtctgcctg gttctgatct ggcgcccggt
     5161 ctgattaccg ggttgcccga aagggcaacc ccgttatttt attagaaacg gaaatcgacg
     5221 ccgacttcga gcagcgtgat gccgtaaccg agcttgccgc gcagagcgat cgagtcgctc
     5281 acgaagtagc gcgcgagacc aaaaactccc cacgcgacac cgctgccggt agctgacgca
     5341 tagctgaaac ccgaaatcga gctctcaacc gatgagctca ccatataata accgagcgaa
     5401 ataccaactg cgagatcgag ttttgggttc ttgatgggta tgtggtaagc gcccgaaacg
     5461 atgatcggta taatcgtgta gctgtatttg acggttgcgc cgaaagtcga gagtgattcg
     5521 ctgtaacccc agtaaccgat cgagccgccg ataccaattt cattggtcag tccgtattcg
     5581 gcatttgcca tcacggcaac cccaccgaga taaccaaggc ctgaaccgag acccagcgca
     5641 ccaccgagaa agaaagcccc cttgtggaat ggctgcccgg aagccggggc cgccgcagtt
     5701 gctgcgggtt tacccttggc tgcaaaaagc gggccgatac ccgatgcgag cagggccagt
     5761 agaagaatag tcaggaattt ttttgatcag ttgtctcctt gagatgtaag tgctagggcg
     5821 atatagaggt gtttttaggc tgtacattgc ttttttgctg caggatcgga gaaactgatt
     5881 tctgttcgac gcgagggcag aaaaaaaatc tatgccccgc taacattcat tatgcaggta
     5941 aacaaaagcg aaagcaaatg gctcgtggtc atcgaagacc aggataacaa actcgggctc
     6001 atcaacatga tacgccacct cgataaggtc gtgagtgagc tcgaaatggc aaaaataccg
     6061 aatctgctgc tcgagataga ccttgcaaaa ctcaactcgg caaactctga actgattgcc
     6121 aaatttgttg agttgcagag catactcgtc cgtaccgatg gcaggcttaa tgttatcaat
     6181 gccaaccccg agctcaagag ctccttcgat gtggttatgc tcgataagat catacctatc
     6241 cagtacgtag ggcaggaaga cccgggcgaa gacggttatg cgtatgacga ctatgagctc
     6301 cccgatgacg atgatgatga cgaagacgac gaagaagaag atggcgatga agacgaataa
     6361 ccggatctct tctgccgcgt tttgacctga tcattttcga ctgggacggt acactctacc
     6421 gttccattga cctcattgaa gcctcgatcg tagctgccgg gcaatcggtc ggcaggcata
     6481 tcagctctgc cgtggcgcgg cacatcattg gcctcggcct gaaagcagca caatcgatcc
     6541 tgtttcctgg cgagcagccc gatgaaaaat ttttgcggga gtttcacctg gcgtaccgca
     6601 ggcactacga ggcgcaggaa aaagacctca gtctgtatcc cggcgcgtac gaactggtgc
     6661 gcgaccttgc tgaccagggt gcgcggattg cggtcgcgac ggccaagagc aggtcgggca
     6721 tcaacagcgc gatggctgcc accggccttg atcgttatgt ttcgtggtcg cgcacacccg
     6781 aagaatgccg gccgaaaccc gacccgcaga tgatcgatga gattgttctc gaggcacggg
     6841 cagaaaaagc caggacgctg atggttggcg acacgacgca tgaccttgcg atggggctca
     6901 atgccggcgt gcatatagcc gggttatgcc atggcgcgca tacagagcca gagctctcaa
     6961 acctgaatcc actggtactg tgcaacgaca tcgcggcact gcgccgcgcc ctgctgtcgt
     7021 aagcgcgcta gccgattttt ctgtagttct tgtattcgcc ggggcggtag cccgtcaggc
     7081 gctctaccag tccagagacc ttctctttga aggtgaagcg cgagcggctg atgtcaaaat
     7141 caaactgcca gttcttgcgc gcgatacgct cgtgcatgac tgccgggtgc gcgcctgtga
     7201 agcgtgtgag agaatcgata ttgctgtagt caaattcagc cgcggacggg atgttttttt
     7261 gcatccagtt gtcgtcgtgc cagtatttat tgaaggtctg ctgcttctgc atctgcgccg
     7321 ccgggggttt tacccaacca tagtggtaga tgccggcgtt tacctggcga acgcgcagct
     7381 tctcattgcc gttcttgcga aaaccctgcg cgtcgcggta agaaaagatg cgcatctgcg
     7441 ggttgagggt aaaatgggga ggtcgcacca cgcgcacttc gcggcggtac cagcggcgcg
     7501 aagcgccggt ataatcgtac gagccgtaaa aatgccggta gtcaaaaagt agtccatcgg
     7561 tccgcgggtc attcgcccac ttctgcatcg cgctgcgtat tgcagggtaa tctttttcgt
     7621 gcagcacttc atcgccctga atataaaatg cccagtcacc gccgtcgatc tgttcgtagg
     7681 ctttttgcgt ctcgagtgcg agcacgcggc caccctcgcg cagcgagtca tcccactcgg
     7741 tttcgataat acggattctg gcggggtcga tatttttgac gagttccagg gtgtcgtcgt
     7801 cagatttgcc caccgcgacg acaatttcgt cgaccagcgg taaaagcgag agcaatgatt
     7861 ccctgatggg gtaatcatag cgaatagcgt tgcgtatgaa ggaaaaaccg gttactttca
     7921 cggataggcg gacgcggggg cagaaatcta ccccctgcgt aaggaaaatg ttattcgtct
     7981 ttcttagact tctttttctt ctttgattct ttcttgtctt tttttgcttt cttttctttt
     8041 ttctcagact tgctgtcgtc gtctttcttg tcttcttttg cttcagcctt gtcttctttg
     8101 tctttcttct ttttgctttt ctttttctta tctttcttct cggccttgtc gtcgtctccg
     8161 tcagctttta ctgcttcgtc agacttttct tttttctttt ttgttttctt ctctgatttg
     8221 tcagagtcgc cctggcctgg cttcgcgcgg cagtatgctt ttactttatc gccgtctttc
     8281 tgcgaataac cgcttaccca tgtgcacttg tcgttcgaag agcattcgct ttcgctcaaa
     8341 cctttgcagg gtgagtctgc tgaaatgcca gcggtgagcg ccatcgccag cgtaaaaatg
     8401 agcgcatggc ccattgagag gagctttttc atagtctttt agagatccgt cgcgacggtc
     8461 actctgtcaa gacgaaatgg ccgctatagg ccgcggttat tcttcttcgc cagtgaccac
     8521 gagctcctgc tggtgcaggt cttcgcgcat gcgcagaccc gccgagtttg caggtttgac
     8581 ttcaagcgga agtaccctgg gtttgccaga gtaccggaac ttctgcacgt actccaggtc
     8641 gctggaatcg ggtatttcgc gtggttcgaa tgccggtttc tgcggcaagg ctacggggtc
     8701 ttcgggagta aaatcgtagt tgtcgatctt ctcgtcgtgc acgtactcgc gctgtccggt
     8761 tttttccatc gcgcgccggc gcagcgagct gacgtattct gctttcgcat gcgggctgag
     8821 ctgcttctca tcggtaagcg gcagatccag ctcaatcgaa acttgtacgt cgcccggtgc
     8881 agatccatca atgattatgg gggctttagg tgccggtttg cgatgcaggg ggcgctgcac
     8941 tccggtagga accgcagcag ttgccggcgc cggcttctgc ctggacacgg cctcttcaat
     9001 caggaaacgc gtctgctctt caatgcccga agtgatttct ccgagtttgc ggcccaggtc
     9061 gctgatcagg gctgcgtctc tgtccttgat ttcgctgacc agggcaaccg agcggtctgt
     9121 taccttaagg gcctgctcat tgagactcaa actcttctcg tagtgctcgc ccagccagcg
     9181 gccctgccgg ttttccagta catcttttac cgtagcccgc agcgagcgaa tgccgcgcac
     9241 ggcgatgacc agtgaaacca ggaggagcaa ggcaacagta ccgcgcagcg cgtagaacag
     9301 gtagccagca aaagggtagc agatgaagag gcgcatgtca ccggcgctca ccggcagaat
     9361 gcgcataccg ttctgcacta ggtaaggcag cgcggtaaat ttatcgcccg atatgaaacg
     9421 ataggcagag tcgggtaact gccgatactc ggcgcggccc gtgagccgca gctcgttgtc
     9481 ggtctgaata aacaggaatt cggaatttgc ctctgtgagg gggtaatttg gtagccgggt
     9541 aacatcccac tctgcatcga gactgaagtt aaaccccgcg ccgctgatcg gtactgttaa
     9601 ataccgtgcg ccgatttcgc gcctggtttc actcgtatca aggtctttca ggcgctcagc
     9661 gttatgagca agcacgacct ttccggcgag tgagcgcagg cgccaggcga cgagggcgcc
     9721 gttgccgaac tggttcagca cacgctccgc ctctttggca aaagccgggg ccagcgaacc
     9781 ttcggtctcg cgcaggaaat acaatatcgc tctttcgttc gccagggttt cgatttgttg
     9841 cgcctgcttt tcgtcggcca cgaaacccga gacatagcgg tgccattcgg ctgcgaggtt
     9901 gcgggcccgg cggtagccgg gttcgagcag cagggtgtcg gcgaggcgca gggcgcgttc
     9961 gccggcagga acctggtctg ccgctgcaat atcggccggg ccggtaaagg gcagcgctgc
    10021 ctgcagtgac gtggtcgtca ccagggcgag cagcaagagg tttctgatgg agtttcgagc
    10081 cggcatgctt gtggggtaat accccagatc attatcgact tccgcaaccg ccaatcttta
    10141 cccggccgaa atttcgggca gggcgaccgg cccggtctac gattctaata gaccgcacca
    10201 ttcgccatcc ggaatttgcc aaggcatggc ggtctctact cactttcctc acggtgacaa
    10261 ggtgctgctg gcggacgtcg atgagggcga tgccctgcag ctggcctctt ttctcgagct
    10321 gaacggctac acggtgcggc gctccacaga aatcaccgag gcgctgacgt atttcagcga
    10381 tttttcgccc ggggtggttg tcattgattc gagcctgccc gcgaaaagct ggcttactct
    10441 catccaggca attcgctcgt atgaagtcga aaaagataca gactaccgca gcatcattct
    10501 cgtcaccgct ggccggttca ccaatgacct gcagcagaag gcaatcaagg cgggggcgca
    10561 cgaagtcatc ggccgccccg tcgacggggc gactctgctc aagcgtatcg cagcgcatac
    10621 cggcgtttac tgacctatgt ttgcccggtt gttacggcaa cgggcgctgc agtttctgct
    10681 gttgtttgtg ggagcttacc ccgttaccgc ccagcgcact gcggttgcgg gcaagcgcag
    10741 tgcgccgaat cctttagggg tttacctgca gctggctgaa agtctcggca tcgaccacac
    10801 ccaccgccgc atcgagtggg attatactct cgattcttat tatacaagcg gcggcatgat
    10861 cattaacttt acagaaaagc cgatacccaa cctcggtgaa gaaagtgaag cgaaggttta
    10921 ccagcacctg ttgacgaagc cgtttgtgcc gcgttatgct gtgctggaag ccagcgtaaa
    10981 cccgatgcct gtcgccggtg ttgtcatacg tgaaaatgcg aacgatgtct accaacgggc
    11041 gcaggtgggc ggtgaaagtt ttaacctcat ccgttcggcg acgcgcggtt tccaggagcc
    11101 ctacgccgtt tcgctctttc tcggtaacgt ggtgcgctac cggccgctcg cgcgcacgcc
    11161 cgaagagcgc gagcagaaca aagcatcggg ttaccagagt tcgctcggtt acagcggtta
    11221 cctcgtgagc tatggcacgc accatatcaa ggataatgaa cttttccagg accactgggt
    11281 tgagctcgag ctcaaggtca agggcgacca gctcttcacg ctgcagaagc tgcggtggga
    11341 ttacaaaatc ggggtgaagt tgcacgataa cccgtttatc tccgacgttg ccgtcatcgg
    11401 tattcgccgt aaccggctcg actatactgc cgacgcctgg tcttttcttg ccaacagcgg
    11461 cttcgagtac cgcattgatt tcaggctggc gaacctcaag ccggtccggc agtattttga
    11521 ggtgaataag aagtggcccg tgaagacggc cgctgcattt tcgctggcgc ttggttttac
    11581 ctgggaatca gagagcctct actccggacc actggataac tatccgggtg gggagcattt
    11641 ccagatattg ttgcggccca acatagagtt ctagctcccc gtattttctg caatacttgc
    11701 ctcggtggca gccctgttgt atctttgcag caatgaactt tgagaagttt aacgcgctcg
    11761 ttgagggccg ggtcaattat ggcgagctcg aaaacgcggc agtggtatcg gaatacaaga
    11821 atattggctg cggcgacggc taccgcctgt acctcgatat tgaaggcgac cagattcgcg
    11881 atgcccgtta cacgaccacg ggctgcagct tctcgctcgc ttcgctggcc attatctgcg
    11941 acctcgccaa gggcaagacg ctagacgccg cactgcagat tgctccggaa gagcttgagc
    12001 ccgcgatcga aggttacccc gagcggcgca agaactatgc cgtgacggcc gtgcaggcat
    12061 tacagaaggc aatccgcgat tttcgcgagc agtcaggcct caagggcgag caggtgatca
    12121 gccgcgataa aaccatcgcg cacctgaaag aacacggcca cctgcgtggc ctgaacctga
    12181 attccgtgat gctcgacaag cttgaccttt cgggtgtgga cttcagcggc tcgaacctgc
    12241 agcatgcctt tttgcgcgaa agcaacctga ccggggcaaa ttttgccggt gctaacctgc
    12301 gcggtgcttt cctgaacgac tgcgttctcg ataagacgaa ctttgccggt gcagacctgc
    12361 gctttgccaa gctgctcggt gcaaagatat cggattgcat ttttgacgga gccctctatg
    12421 atattggtac ccgcgtcgac acgaagaata tgcacatttt cgaggtgatg acgcagaagg
    12481 gtaaggaact ctacctcaaa ggcggagcgc aggctgtaac agcctaaatg tgttgtcagc
    12541 ctgtctcaac aggcaactct gcccgcacac cgcgcggtag agcagttggt agctcgttgg
    12601 gctcataacc caaaggtcgc aggttcaagt cctgcccgcg ctaaagttta ggttattggt
    12661 gggcgtagcc caccaataac ccatatttgg cgacgtagct cagctggtta gagcgatgga
    12721 atcataatcc acaggtccgg ggt
//
LOCUS       DAOQJF010000047        12584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2468_length_12584_cov_5.384308, whole genome shotgun sequence.
ACCESSION   DAOQJF010000047 DAOQJF010000000
VERSION     DAOQJF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2468_length_12584_cov_5.384308"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLF85_04065"
     CDS             complement(<1..246)
                     /locus_tag="PLF85_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18863.1"
                     /translation="MAKIIAFLLFASLPVLADFDTETLPAELAARTKEFLGADTGAAR
                     SGMAKFSDDELQKITTAFKKVHSKEEQRLFWLSEELYR"
     gene            complement(239..928)
                     /locus_tag="PLF85_04070"
     CDS             complement(239..928)
                     /locus_tag="PLF85_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HNE18864.1"
                     /translation="MAFTRSPLLFGALAITAMAVCAWSAWLALIVAPADAAQGNIQRV
                     FYFHVPLVWVSFLAFIGGAIMSIRYLRGRNSLDDAWAQAFIKTGWVFTSTVLVTGPLW
                     ARPVWGIFWNWGDERLVSFFVMWMMYNGYLLLRVTIADQEKAARIGAVLAILALMNAA
                     LVVGAIYIWKTASHPGPVFVKQGGSGLADPTMKLAFWSSFAGFTLLFVTLLITRFSVE
                     KQLVETNETHG"
     gene            complement(931..1971)
                     /gene="mtnA"
                     /locus_tag="PLF85_04075"
     CDS             complement(931..1971)
                     /gene="mtnA"
                     /locus_tag="PLF85_04075"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNE18865.1"
                     /translation="MKAHLSFIKTARDKIQLLNQKLLPHKLDYVTCQNGNDVAKAIKD
                     MVVRGAPAIGISAAYGMYLTAWEASKKNKKLTPDLLKKQKQALDAARPTAVNLAWATG
                     EMLAVANTFFASEASKAKDANLKLLLQLYEKALEIHNDDAERCLAMSNNAVKYLLTKY
                     PKEKYNILTHCNTGSLATGGIGTALGAIRVLHEKGKINMVYADETRPYLQGSRLTVFE
                     LQKDKIPVTLTVDSQAAYLMQKKLVDAVYVGADRVAANGDVANKIGTYGLSVLAKAHR
                     IPFFVVAPKSTFDDSIQTGEKIVVEERSPVEVLEIAGRVIAPKVPVVNYSFDITPQAN
                     ITAIFSEDDTLE"
     gene            complement(2015..3556)
                     /gene="lysS"
                     /locus_tag="PLF85_04080"
     CDS             complement(2015..3556)
                     /gene="lysS"
                     /locus_tag="PLF85_04080"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNE18866.1"
                     /translation="MSDTTPSGANELEQQRLQKLNAIREKGVDPYPVRFPGKEDLQAI
                     RLQHFGAEGELTPPEDATQVTTAGRVVAIRDMGKAQFLQIQDQSGRMQVYAGNKTLNE
                     EDYFVLRQLDLGDIIGIVGKPFRTKTGEPTIHADRLSILAKNLNPLPVVKEKDGETYD
                     GISDIELRYRQRYIDLAVNADARSTFALRSAILREIRDFLHQRGFMEVETPMMQAIAS
                     GAAARPFTTHHNALDIDLFLRIAPELYLKRLIVGGYEKVFEMNRNFRNEGISTKHNPE
                     FTMIEIYQAYADYNTMMELTEEIFETLALKLHGKTEIPYGTHTVSLKRPWKRVSYLDS
                     IAEATGLDFNKFLGEENPSAAEAKKMATSIGLKAEALHTFWEVVDLVFSEKVEPNLIQ
                     PTIVMHYPKAISPLAKSIPGNPHLVERFEPYITGREMGNAFSELNDPHEQELRFREQA
                     RQKDSGAEETMATDDDFVTALKVGMPPTGGLGIGIDRLVMLFANKASIKDVILFPLLR
                     PNRQT"
     gene            complement(3618..5129)
                     /locus_tag="PLF85_04085"
     CDS             complement(3618..5129)
                     /locus_tag="PLF85_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18867.1"
                     /translation="MGFFSFQPEFNYDGVNASDSVEASKHLLPFKGGFLKGLEAHLVN
                     ADNVQKFLASAPPETRKEINKNIKDIDSALVAIMRLYGNIENELSTRTLDTAAHREYY
                     NHFSDADIEEPKLDYVRLIRSYELIKEFNLAVIEHWWKVANIFESFATPSSEKHFLEQ
                     LLVYRIYPRIDLCNLTNLLLRRIAYVLKIDSEETVGGRVTVRGHYSSQISYSYSVVFR
                     EDISEIIEGLEAVNRGHEFKARAPEAIKETRDTAIKSPYLLDTRGTEPFNQTHAYLLE
                     INQSRFTLEEAKVGRSIYVETHLGAEDDALRQDLIRKFISAARNKDRVGEYRTFLHSY
                     FQFTRDTLLMQEQRIPEADHTFFLYHFGPVFFVKVCTHFMRIGRTGYVHRHLKRNQMV
                     RELPYEYVKYVLKDWWDENVYTQVPRAERNSHVAYERLLDTIRTEWQEHQLRLLVRIK
                     KEPPLLRALNIHDVKTLGAFVENEISYIYHVKILRFLGQDFFYLPLHVKSHQA"
     gene            complement(5138..8236)
                     /locus_tag="PLF85_04090"
     CDS             complement(5138..8236)
                     /locus_tag="PLF85_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18868.1"
                     /translation="MQEYPTPETPRTTPFARVFSALGKTFSVTGYSLLGVLLLAVIGY
                     NYVMRPDFLQPRIVEEFAKATHGRLELKLEQASLFRGFRIRNIAVHPPEGYSATPIFK
                     ADEINILYNVFGFFRGSFGVHEILLRNPEVFIEQRNNVFNVQALPKPSEKKEKPEEEK
                     EPADRKPGSDVISWFFNVHLFAHLALENLNFTLDATDESNKVKRYAHLKNFHFRFSLL
                     TRNFSSVNKADPAKLVQLLNAFVIELNPQKKIQLAYEGPEARVRTDLDMFWLLFYDGA
                     TEQPEFISRMRIGQDKIPVALGKGRTQNLAFVSEHAIDYDARADKLDISAFAVRFMGD
                     TLLSLTGNGERLLKKDRRIAIATGESRVNLGKLYEVNAQLLGKRDPYFSGFFSIKPTK
                     VNISGNTIDDSGGVKLDRVQVRQGKLSLSIPSLDLDHAAFVDSTLGPLPVRQASAKMR
                     GNFNGAGIALDASLAEDKKTAVSFSLRGLDLTPFAAGAAAGTISTTFSATGPSPQDLA
                     IAFRLFSPQLFYYVDRGKSGINRIDLNIRGIVKSSENFKQNSINLPVINLSNKDKEYG
                     AAVDLKSRAQIEKADAIRVTYNLDGLTVAFKELVNTMPAALQESLSGLLKSLKPGKSL
                     RADGETSVSIGGGVTQVEHLTQLALPDVHVDDIRIKAKARLAPPMTYLDQFSITGLRQ
                     ALQVSASGNLRDGSETVQDEKTGKQLRVQTKVPDIRFKAELSRKEESEILDDMFLVGS
                     FLLVGHAQGNIVDGKVQIDNLGFRNPQSRVQKVNLLFPFKHDLKLKKTLNLRAGNKER
                     IIKNYNFNRPYNFSIENIEIPDPNKKGEWLKLVYARGNYPAVGASMEYKDNVFVMPVL
                     QMYTLNGVVTISDTLFNLGRLRPSEMEYSTTIQIKDIDLKPLMVKEKADTITDGKLRI
                     DILLTGNRLDKPVENLNGYLSIFRIGPEFAEAVMKAVKPKQSDIINTIATNTAIPKKI
                     NIELRDGFVYSDIPIKKGAVGALLFSPDEINNRRINIPEFFQRISTEASTYTSPAANP
                     GN"
     gene            complement(8238..8867)
                     /locus_tag="PLF85_04095"
     CDS             complement(8238..8867)
                     /locus_tag="PLF85_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18869.1"
                     /translation="MVRLSSPTIFMNGRVFKHAHQKFCLARITLLSISLPLLTTALSA
                     DAAYDALKGQVKGLYSSFRATFAVTSSSGQIQTGKIMYQYPGKLRVETGSGVIATNGT
                     HLWVHNKNSPLCARQDVSGVGGGILGILASYEGTIQGNTYVFRKKGAHYDEIVVQVAG
                     GMLRSVRLKHGDETTTYSFSGMTVDTGISGSMFNYKPAGAQIVENPLNN"
     gene            8886..9446
                     /locus_tag="PLF85_04100"
     CDS             8886..9446
                     /locus_tag="PLF85_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18870.1"
                     /translation="MIACKAPFALLFLLLSATAVSGHPSFDYPATRLFPFDRGTPDYS
                     QRLLSENVRFLLFRAEFKVRQTGYANSSGSLANLQLATNFAALASFGLAGLAPEELDR
                     DLYNAERFTVRLYESMARRWERLNPIPGLLYEVVIRSPAYPFIFMEGLDAYWFDLGTF
                     PFTDDLLFRELSLASRQYGFGFRISF"
     gene            complement(9488..10468)
                     /locus_tag="PLF85_04105"
     CDS             complement(9488..10468)
                     /locus_tag="PLF85_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNE18871.1"
                     /translation="MSNTAESSAESKYRKVSSPYGFSMYDVEATAAIEKASRAAQEIV
                     KAHGFERIMPAMVDYPETFSESQHSEMFSLKDNSGERLALRNDITAQVIKGYVRQIKR
                     KSGVNLRKFYYAAPVYKDVRKNYPLPREIWQIGCEIVGENADSHLAELLTISQRIMRE
                     VFGHETATQIADVRLRNIFAELAGPDYPAAERRRDAPFLVRILTDEFSVTEAEARELA
                     LWFFYPTPEKKIPQAVASLPDILQAAIGEAAAEAEKTIRLLAAAGITAIWQPLCEPRS
                     SYYSGLFFETYARGFTEPLVRGGVYNELVSRYAQIEAGACGFALDILQFS"
     gene            complement(10465..>12584)
                     /gene="glyS"
                     /locus_tag="PLF85_04110"
     CDS             complement(10465..>12584)
                     /gene="glyS"
                     /locus_tag="PLF85_04110"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNE18872.1"
                     /translation="LLAMAETLLFELATEEIPAAYQKNLWLDWERRLPQLLATTGLGH
                     GKISVFASARRIAFCVEAIDERAEDVTETLQGPAKNVALTADGKPTPALVGFAKKAGL
                     EPAEVTFTESPKGAYATATVVRKGKTLREALPGLLHDLCGNYRFPRSMRWADQGNVYA
                     RPIAGYFLLYGDKQWQLDEAELKGTVLESIPFRSVRGHFILDTAPLQVPAASAYASTL
                     HGHGIVADPRVRRAGIAQLLTEAASAEGLSLIANDSLLDEVNFLVERPSIVVGSFSED
                     YLRLPDGVILSEMNQHQRYFGLRDKQGKLSNRFLIVANADTKNPAAVQNIRSGNERVL
                     RARLSDGAFFYDEDLKRSLADRVDDLKQIVFHEGLGTYLEKVRRIGELAAIFGGDKAE
                     LKQLTEAARLIKADLTTQLVYEFDHLQGEIGAVYAARNGVNPETCTAIHEHYLPRFQG
                     DALPSTRLGTLLSLADKWDNTVAAFLLGKEPTASQDPFAIRRQSLYIIQMLVAQKIRL
                     SLSDLLKESIRIYAADYKGKADAEQLREKIVQFFKARLVTIFEGEGFDKRLTRAAVFS
                     GSDDAYDLFTRAAALRDIAEKDRSSFDALLAAFKRMANIIKEVKNAGPVNTGLLSQTE
                     ELALHAFAQKLHGMADEAVSDPLVHYRAIFSEFAAGKPIVDAFFDKVMVNHEDQAIKA
                     NRQNLIAYTLAAVTQLIDLEQLS"
BASE COUNT         2552 a         3316 c         3642 g         3074 t
ORIGIN      
        1 gcggtaaagc tcttcgctga gccagaacag gcgttgttct tctttactgt gaactttttt
       61 gaacgcggtg gtaatcttct gtaattcatc gtcgctgaac ttcgccatac cactgcgcgc
      121 ggcaccggta tcggctccca ggaactcctt cgtgcgagct gcaagctcag caggcagagt
      181 ttctgtatca aagtccgcca gtactggcaa cgatgcgaac agaagaaatg cgattatttt
      241 agccatgagt ttcattggtt tccaccagtt gtttttccac gctgaagcgg gtgatgagta
      301 atgtgacaaa aagcagcgta aagccggcga acgatgacca gaacgccagt ttcatcgttg
      361 ggtcagcaag tcccgagccc ccctgtttga cgaacacggg tcccgggtgc gaagcagtct
      421 tccagatata gatcgcgccg acgaccagcg ccgcattcat cagcgcgagg atggccagta
      481 ccgcaccgat gcgcgcggcc ttttcctggt cagctatggt cacgcgcagc agcaggtaac
      541 cattgtacat catccacatg acaaaaaaag atacaagccg ttcatcgccc cagttccaga
      601 agatacccca gacgggtcgt gcccagagcg gcccggtgac aagtaccgtc gaagtaaaaa
      661 cccagcctgt cttgataaat gcctgtgccc acgcgtcgtc gaggctgttg cgcccgcgaa
      721 ggtacctgat gctcatgatc gctccgccga tgaaggcaag aaacgaaacc cagacaagag
      781 gcacgtgaaa gtaaaagacc cgctgtatat tgccctgcgc tgcgtcggcc ggcgccacga
      841 tgagtgccag ccatgcagac catgcgcaga cggccattgc cgtgatcgcg agcgcgccaa
      901 agagtaaggg tgaccgggtg aatgccatgc ttattcgagc gtgtcgtcct ctgagaagat
      961 ggccgtaata ttcgcctgcg gtgtaatgtc aaaactgtaa ttgactaccg gcactttggg
     1021 tgcaataacg cggcccgcga tctcgagcac ctcaacgggg gagcgttctt cgaccacgat
     1081 cttttcgccg gtttggatag aatcatcgaa ggttgatttt ggggcaacca cgaagaacgg
     1141 aatacggtgt gccttggcga gaacggaaag gccgtaagtg ccgatcttgt ttgccacgtc
     1201 gccgttggcg gcaacacggt cggcaccgac gtaaacagca tcgacgagtt tcttctgcat
     1261 caggtacgcg gcctgagaat caacggtgag cgttacggga attttgtctt tctgtaactc
     1321 gaagacggtc aggcgcgagc cctgcaggta tggccgggtt tcgtcggcgt aaaccatgtt
     1381 gatcttgcct ttttcgtgca gcaccctgat cgcgccaagg gcggtaccga taccgccggt
     1441 ggccagcgag cccgtgttgc agtgcgtgag aatgttgtat ttttctttcg ggtactttgt
     1501 cagcaggtac ttcactgcgt tgttcgacat tgcgaggcag cgttcggcgt cgtcgttgtg
     1561 tatctcgagc gctttctcgt agagctgcag cagcagtttc aggttggcgt ctttcgcttt
     1621 cgacgcttcc gaggcaaaaa aagtattggc aacagccagc atttcaccgg tagcccaggc
     1681 gaggttgacc gcggtcgggc gcgcagcatc gagtgcctgc ttctgttttt tcagcaggtc
     1741 gggcgtgagt ttcttgtttt ttttcgaggc ttcccatgca gtcaggtaca taccgtacgc
     1801 cgcgctgatg ccgatggccg gagctccgcg taccaccatg tccttgatag ccttggcaac
     1861 atcgttacca ttctggcagg tgacgtagtc gagcttatgc ggcagcagct tctggttgag
     1921 taactgtatc ttgtcgcgcg cagtcttgat gaacgagaga tgagctttca tgttgtttcc
     1981 tctgtttttt ttcagccttc ttgcatagca actatcaggt ttggcggttg ggtcgcagca
     2041 acgggaaaag tatgacgtcc ttgatactgg ccttgttggc aaagagcatg acaagcctgt
     2101 cgataccgat gccgagcccg cctgtcggcg gcatgccgac tttcagcgcg gtgacaaaat
     2161 catcatcggt cgccatggtt tcttcggcgc cgctgtcttt ttgccgggcc tgctcgcgaa
     2221 agcggagttc ctgttcgtgc gggtcgttca gctcgctgaa agcgttgccc atttcgcgtc
     2281 ccgtaatgta aggctcgaag cgttcgacga ggtgcgggtt tccgggtatc gatttcgcga
     2341 gaggtgaaat cgctttgggg taatgcatca caatcgtcgg ctgtatcagg ttcggttcaa
     2401 ccttctcgga gaacaccagg tcgacgactt cccagaaagt gtgtaacgct tctgccttga
     2461 gcccgatcga tgtcgccatt ttcttggctt cggcggcaga gggattttct tcgccgagga
     2521 acttgttaaa gtcaaggcct gtggcttccg caatactatc gagatagctt acgcgtttcc
     2581 agggacgctt cagagatacg gtgtgtgttc cgtaagggat ttctgttttg ccgtgcagct
     2641 tgagcgcgag cgtttcgaat atctcttcgg tgagttccat catcgtgttg tagtcagcat
     2701 aagcctggta gatttcgatc atggtgaatt cggggttgtg cttggtgctg ataccttcgt
     2761 tacggaagtt ccggttcatc tcgaagactt tctcgtagcc gccgacaatc aggcgtttca
     2821 ggtaaagctc cggcgcgatg cgcaggaaaa ggtcgatatc cagcgcattg tggtgcgtgg
     2881 taaaaggccg cgccgcggct cccgaagcga tcgcctgcat catcggggtt tccacttcca
     2941 taaaaccccg ctggtgcaaa aagtcgcgaa tctcgcgcag tattgccgag cgcagcgcaa
     3001 atgtcgagcg cgcgtctgca ttcaccgcga ggtcaatgta gcgctggcgg tagcgcagtt
     3061 caatgtcgct gatgccgtcg taggtctctc cgtccttttc tttgacgacc ggcagcgggt
     3121 tcaggttttt tgccagtata gacaggcggt cagcgtgaat cgtcggctcg ccggtcttgg
     3181 tgcggaaggg ttttcccaca atgccgatga tgtcgcccag gtcgagctgg cgtagtacaa
     3241 aatagtcctc ttcgttcagg gtcttgttgc cggcatagac ctgcatacgg ccgctctggt
     3301 cctgtatctg caggaactgg gccttaccca tgtcgcgaat cgccacaacc cggccggccg
     3361 tggtgacctg tgtcgcgtct tcgggaggag tgagctcgcc ctcggcgcca aaatgctgca
     3421 gccgtattgc ctgcaggtct tcttttccag gaaacctgac ggggtatggg tcgacaccct
     3481 tttcccgtat tgcattcagc ttctgcaggc gctgttgctc aagttcgttg gcgccagaag
     3541 gcgtagtatc gctcattata cgccaaaacc gggttttttg acgcgctgta aaggattggg
     3601 gccgctcggc cgaaggctca ggcctggtgc gatttcacgt gcagcggcag gtagaaaaag
     3661 tcctgcccca ggaaacgcag tattttcacg tgatatatat aagatatttc attctccaca
     3721 aaagcgccca gcgtcttcac atcgtggata ttgagcgcac gcagcagtgg cggctctttc
     3781 ttgatgcgaa cgagcagccg cagctggtgc tcctgccact cggtacggat ggtgtccagc
     3841 agtcgctcgt aggcgacgtg gctgttccgc tctgcccgcg ggacctgcgt gtaaacgttt
     3901 tcatcccacc agtccttgag cacgtacttc acatattcat aaggcagctc gcgtaccatt
     3961 tggttgcgct tgaggtgccg gtgtacataa cctgttctgc cgatgcgcat gaaatgcgta
     4021 cagaccttga cgaagaaaac cgggccaaag tggtacaaaa agaaagtatg gtctgcttcg
     4081 ggtatgcgct gttcctgcat gagcagggtg tcgcgcgtga actggaagta gctgtgcagg
     4141 aaggtacggt actcgccgac ccggtccttg ttgcgagcgg cggagataaa cttacggatg
     4201 aggtcctgcc gcagtgcgtc atcttctgcg ccgaggtggg tctcgacgta aattgagcga
     4261 ccaaccttgg cttcttcgag cgtgaaccgt gattggttga tctccagcag ataggcgtgc
     4321 gtctggttaa agggttcggt gccgcgtgtg tcgagcaggt aaggtgattt gatcgcggta
     4381 tcgcgggttt ctttgatcgc ctcgggagcc ctggccttaa attcatggcc acggtttact
     4441 gcctcaaggc cctcgatgat ctccgagata tcttcgcgaa agacgaccga gtaagaatag
     4501 ctgatctggc tgctgtaatg accgcgcacc gttacgcggc caccgacggt ttcttcggag
     4561 tcaattttca gcacataggc gatgcggcgc agaagaaggt ttgtcaggtt gcagaggtcg
     4621 atacgcgggt agatgcggta tacgagcagt tgctcgagaa agtgtttttc agaactaggt
     4681 gtggcgaacg actcgaagat attggcgact ttccaccagt gctcaatgac ggcgaggttg
     4741 aactccttga tgagttcgta gctccggatc aggcggacgt agtccagttt gggttcttca
     4801 atatctgcgt cggagaaatg gttgtagtac tcacggtgcg ccgcggtgtc cagcgtgcgg
     4861 gttgataatt cattctcgat attgccgtag agccgcataa tagcaaccag tgcagaatcg
     4921 atatccttga tgttcttgtt gatttcttta cgtgtttcgg gcggcgccga ggcaagaaat
     4981 ttctggacgt tgtctgcgtt gacgaggtgt gcctcgaggc ctttgagaaa tccgcccttg
     5041 aatggcagca ggtgcttgct cgcttcaact gagtcagagg cattgacccc gtcgtagtta
     5101 aattccggct ggaaagaaaa aaatcccact catgacctca gttacccgga ttcgcggcag
     5161 gggatgtgta cgtgctcgct tcggttgaaa tgcgctggaa aaattcggga atgttgatgc
     5221 gacggttgtt aatttcgtcc ggcgaaaaca gcagcgcacc gactgcccct ttcttgatcg
     5281 gaatatccga atagacgaag ccgtcgcgca gctcaatatt aatcttcttc ggaatcgcgg
     5341 tattggttgc gatcgtgttg atgatgtcag attgcttggg tttaactgcc ttcataaccg
     5401 cttcggcaaa ttcgggtccg atgcggaaaa tagaaaggta gccgttcagg ttctcgacag
     5461 gtttgtcgag gcggttacca gtcaaaagaa tatcgatacg cagcttgccg tcggtgatgg
     5521 tgtctgcctt ttctttgacc atcagcggtt tgagatcaat gtctttgatt tggatggttg
     5581 tcgaatattc catctcgctt ggcctgagcc tgccgagatt gaacagcgta tccgagatgg
     5641 ttaccacgcc attcagggta tacatctgca gcaccggcat gacaaaaaca ttgtctttgt
     5701 attccatcga cgctccgact gctgggtagt taccgcgggc gtagacgagc ttgagccatt
     5761 cgcccttctt attcgggtcg ggtatctcaa tattctctat cgaaaaattg taaggtcggt
     5821 tgaagttata gtttttgatg attctttctt tattgcccgc ccgcaggttg agggttttct
     5881 ttagcttcag gtcatgctta aaggggaaaa gcaaattcac tttctgcacc cggctttgcg
     5941 ggttccggaa acccaggttg tcgatctgca ccttgccatc gacgatgtta ccttgggcat
     6001 ggcccacgag caagaagctg ccgaccagga acatgtcatc gagaatttcg ctttcttctt
     6061 ttcgactgag ttcagccttg aagcggatgt ccggcacctt ggtctgcacg cgcagctgtt
     6121 tgccggtttt ttcgtcctgt accgtctcgc tgccgtcacg caggttgccg ctggcgctca
     6181 cctgcaaagc ctgtcgcagc ccggtaatgc tgaactggtc gaggtaagtc attggcgggg
     6241 ccaggcgagc ctttgccttg atgcgtatgt cgtcgacatg aacgtcgggt agtgcgagtt
     6301 gtgtgaggtg ttcgacctgc gtgactcctc cgccaatcga gacagacgtt tcaccgtctg
     6361 cgcgcagcga cttaccgggt ttgagagact tcagcagccc gctcaagctt tcctgcagtg
     6421 cggcgggcat cgtattcacc agctctttga atgcaaccgt cagcccgtcc aggttgtagg
     6481 tgacgcgtat cgcgtcggcc ttttcaatct gcgcccgcga tttgagatca accgctgccc
     6541 cgtattcctt atccttgtta gataggttaa tcacaggcag gttgatcgag ttttgtttga
     6601 agttttcgct gcttttcact atgccgcgga tattcaggtc aatgcggttg atacccgatt
     6661 tgccgcggtc gacataataa aaaagctgcg gcgagaacaa acggaacgca atcgcgagat
     6721 cctgtggcga ggggccggtt gcgctgaagg tagtactgat ggtacctgct gcagcgcccg
     6781 cagcaaatgg cgtaagatcg aggccgcgca gtgaaaagct gacggcagtt tttttgtctt
     6841 cggcgagcga ggcatcgagc gcaattcctg cgccgttgaa gttgccgcgc atctttgccg
     6901 atgcctgccg cacaggaagc ggtccgagcg ttgaatcaac gaaggctgcg tggtccaggt
     6961 cgagcgaagg tatcgacagg gagagttttc cctggcggac ctgtacgcga tcgagtttaa
     7021 ctcccccgct gtcatcgatg gtgttgccgc tgatattcac cttcgtgggt ttgatcgaga
     7081 agaagccgga aaaataaggg tcgcgtttac ccaaaagctg ggcattcacc tcgtaaagtt
     7141 tacccaggtt gacccgcgat tcgccggtcg cgatggcaat gcgccggtct tttttcagca
     7201 gtctctcacc gttacccgtg agcgagagca gtgtgtcgcc catgaagcgc acggcgaacg
     7261 cagaaatatc gagtttgtcg gcgcgcgcgt catagtcgat cgcgtgttct gagacaaacg
     7321 ccaggttctg cgtgcggcct ttacccagcg ccacgggtat cttgtcctgg ccaatacgca
     7381 tgcgtgaaat gaactcgggt tgctcggtgg cgccatcgta aaagagcagc cagaacatat
     7441 cgagatcggt acgaacgcgc gcttcgggcc cttcgtacgc cagctgtatt ttcttttgtg
     7501 gattgagctc gatgacaaag gcattcagca gctgcacgag ttttgccggg tcggccttgt
     7561 tgacgctgct gaaattgcgt gtgagcagcg agaaacggaa atgaaaattc ttcaggtgtg
     7621 cataacgctt caccttgttc gactcatcgg tggcatcgag tgtaaagttc aggttttcga
     7681 gggccaggtg ggcaaagaga tgtacgttaa agaaccacga aatgacatcg ctacccggtt
     7741 tacggtcggc aggctctttt tcttcttcgg gtttttcttt tttttccgaa ggtttcggca
     7801 gcgcctgtac gttgaagacg ttattgcgct gctcaatgaa tacttccggg ttgcggagca
     7861 gaatttcatg cactccgaac gaaccgcgaa aaaaaccaaa aacattgtag aggatattga
     7921 tttcgtctgc cttgaagatc ggtgtcgccg aataaccttc aggcgggtgc actgcaatat
     7981 tgcggatgcg gaacccgcgg aagagcgatg cctgttcgag cttcagttcg agccggccgt
     8041 gcgtcgcttt agcaaactct tcgacaatac gcggctgcag gaaatccggc cgcatgacgt
     8101 agttgtaacc gatgactgcc agcaacagca cgcctagcag gctgtagccg gtaacagaga
     8161 aggttttccc gagagccgag aagacgcgtg cgaaaggggt ggtccgtggc gtctctggcg
     8221 tcgggtattc ctgcatctca gttattcaga ggattctcga cgatctgcgc gcccgcaggt
     8281 ttgtaattga acatggatcc cgagataccg gtgtcaacgg tcatgcccga aaaagagtaa
     8341 gtcgtcgtct cgtcgccgtg tttgaggcgc acgctgcgca gcataccacc cgcaacctgc
     8401 acgacgattt catcgtagtg cgcccctttt ttgcggaaca cgtaagtatt gccctgtatt
     8461 gtgccttcgt acgaagcaag tatgcccagt atgccaccgc cgacgcctga aacatcctgc
     8521 cgcgcgcaga ggggggagtt cttgttgtgc acccaaaggt gagtaccgtt cgtggcaatg
     8581 acgcctgagc cggtttcgac gcgcaatttg cccgggtatt ggtacatgat cttgccggtc
     8641 tgtatctgcc ctgaagacga ggtgacagcg aaggtcgcgc gaaaagacga gtacagcccc
     8701 tttacctgtc ctttgagcgc gtcgtaggcg gcatcggcag aaagggcggt agttaacagg
     8761 gggagcgaaa tcgacaggag cgtaatacgc gccaggcaga atttttggtg cgcgtgcttg
     8821 aagactctgc cgttcataaa gatggttggt gagctcagtc gaaccatacg aaggcttata
     8881 cgctgatgat tgcctgtaaa gcacctttcg ctctcctgtt tctgctgctc agcgccacag
     8941 ctgtgtccgg gcatccttcg ttcgattacc cggcaacccg gctttttccc ttcgaccggg
     9001 gtactcccga ctattcgcag cggctcttga gcgaaaatgt ccgctttctg ctcttccggg
     9061 cggagttcaa ggtgcggcag acgggttacg ccaacagttc gggtagcctc gccaacctgc
     9121 agcttgccac aaactttgcc gcgctggcaa gcttcggcct tgccggactg gcacccgaag
     9181 agctcgaccg cgacctctat aatgccgagc gctttactgt gcgcctgtat gaaagtatgg
     9241 cgcggcggtg ggaaaggctc aaccctattc ccggcttgct ctacgaagtt gtgatccgca
     9301 gtcctgcgta tcccttcatc ttcatggaag gcctcgatgc ctattggttt gacctgggta
     9361 cttttccttt tactgacgac ctgcttttca gggagctcag ccttgcctcg cgccagtacg
     9421 gctttggctt tcgcataagt ttttagcaat gctccgaaaa agtcgcactt tgtgcgattt
     9481 ttcgtgccta agaaaactgc aagatatcga gggcaaagcc gcatgcgccg gcttcaatct
     9541 gggcgtagcg gctgacgagt tcgttgtaaa ctcccccgcg caccagcggt tcagtgaaac
     9601 cgcgcgcata tgtttcaaag aacaggccgc tatagtagct actgcgcggt tcacacagcg
     9661 gctgccagat tgccgtaatg ccggcagctg ccagcagccg gatcgtcttt tcagcttcgg
     9721 cggcggcttc accgatagca gcctgcagaa tgtcgggcag agatgctaca gcctgcggaa
     9781 tctttttttc cggcgtcggg tagaaaaacc agagcgcaag ttcgcgtgct tcggcctcgg
     9841 tgacgctgaa ttcatcggtc agaatgcgca ccaggaatgg agcgtcgcgc cggcgctcag
     9901 cggcggggta gtcaggtccc gcgagttcgg cgaagatatt gcgcagcctg acatcggcaa
     9961 tctgcgtggc ggtttcatgc ccaaaaactt cgcgcatgat gcgctggctg attgtcaata
    10021 actctgcgag gtggctgtcg gcattttcac cgacgatctc gcagccaatc tgccagattt
    10081 cccgtggtag tggatagttc ttgcgcacgt ctttgtagac aggcgccgcg tagtagaact
    10141 tgcgcaggtt aacgccgctc ttgcgcttaa tctgccggac ataacctttt atgacctgtg
    10201 cggtaatatc gttcctgagc gccaggcgtt cgccgctgtt atctttcaga ctgaacatct
    10261 cgctgtgctg gctctctgag aatgtctccg ggtagtcgac catcgcgggc ataatgcgct
    10321 cgaaaccatg agccttaaca atttcctgtg cggcgcggct ggccttttcg atcgccgccg
    10381 ttgcctcgac gtcgtacatg ctgaagccat acggcgacga gaccttgcgg tatttcgatt
    10441 ctgcgctcga ttctgcagta ttgctcatga gagttgctcc aggtcgatca gctgtgtcac
    10501 tgccgcgagt gtgtaggcaa tgaggttttg gcggttcgcc ttgatagcct ggtcttcgtg
    10561 gttaaccatg accttatcaa agaatgcatc gacgatcggc ttacctgcag cgaattcgct
    10621 gaagatggcc cggtagtgca ccagcgggtc ggataccgct tcatcggcca tgccgtgcaa
    10681 tttctgtgca aaagcatgca gagccagctc ttcggtctgc gacaagaggc cggtattgac
    10741 cgggccggca ttcttcactt ctttgatgat atttgccatg cgcttgaagg ctgcgagcag
    10801 cgcgtcaaag ctactgcggt ccttttctgc gatgtcgcgc agcgcagcag cccgcgtaaa
    10861 caggtcgtaa gcatcatcag accccgagaa gacggccgct cgcgtcaggc gtttgtcaaa
    10921 gccttcacct tcaaagatcg ttaccagccg cgccttgaag aactgcacga tcttttcgcg
    10981 cagttgttca gcatctgctt tacccttgta atctgcagca tagatcctga tcgattcttt
    11041 gagcaggtct gagaggctca ggcggatttt ttgcgcgacg agcatctgga tgatgtagag
    11101 cgactggcgc cgaattgcga atgggtcttg cgacgcggtc ggttctttac cgagcagaaa
    11161 agcagccacg gtgttgtccc atttatcggc cagcgagagc agcgtgccga gcctggtcga
    11221 cggaagtgca tcgccctgga aacggggcag gtaatgttcg tgaattgctg tgcaggtttc
    11281 gggatttact ccgttgcgag cagcataaac agcgccgatt tcgccctgca gatggtcgaa
    11341 ttcatacacc agctgggtcg tgaggtcagc cttgatcagg cgtgccgcct cggtcagctg
    11401 cttgagctcc gccttgtcgc cgccgaagat tgccgccaat tcgccaatac gccgcacctt
    11461 ttcgaggtag gtgcccagcc cttcatggaa cacgatctgc ttcaggtcgt cgacgcgatc
    11521 ggccagcgag cgcttcaggt cttcgtcgta gaagaatgcg ccgtcagaca ggcgcgcgcg
    11581 taagacacgt tcgttaccac tgcggatgtt ctgcactgca gcggggtttt tcgtgtcggc
    11641 gttggcgacg atgagaaacc ggttagagag tttgccctgc ttgtcgcgca ggccgaaata
    11701 gcgctggtgc tggttcatct ctgacaagat gacgccatcg ggcaggcgca ggtaatcttc
    11761 ggagaaagat cctacaacaa tcgaagggcg ctcgaccagg aagttgactt cgtcgagcag
    11821 cgagtcgttg gcgatcagcg aaagtccttc ggcagaagct gcttcagtga gcagctgcgc
    11881 gatacctgcg cggcggactc gcgggtcggc aacgataccg tggccgtgca gcgtgcttgc
    11941 ataagcagaa gcagcgggta cctgaagcgg agctgtatcg agaataaaat ggccacggac
    12001 gctgcgaaac ggaatcgatt cgagcactgt ccctttgagc tccgcttcgt caagctgcca
    12061 ctgcttgtcg ccgtagagca gaaagtaacc ggcgatgggg cgcgcgtaga cgttgccctg
    12121 gtctgcccag cgcatcgacc gcggaaaccg gtagttgcca cagagatcgt gcaagaggcc
    12181 cggcagagct tcgcgcagtg tcttaccttt gcgcacgacc gttgctgtgg cgtacgctcc
    12241 tttgggtgat tcggtgaacg tcacctctgc cggttcaagg ccggctttct tggcaaagcc
    12301 gaccagtgct ggcgtgggtt tgccatcggc agtcagcgcg acattttttg ccggcccctg
    12361 cagtgtttca gtgacgtctt cggcacgctc gtcgatagct tcgacacaga aggcgatgcg
    12421 gcgcgcactg gcgaatacgc tgattttacc gtgcccaagg cccgtggttg ccagcagctg
    12481 cggcaggcgg cgctcccagt cgagccagag gttcttctgg tacgcggcgg gaatttcttc
    12541 cgttgccagt tcaaagagca gggtctcagc catcgcaagc aaaa
//
LOCUS       DAOQJF010000048        12350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2546_length_12350_cov_5.774868, whole genome shotgun sequence.
ACCESSION   DAOQJF010000048 DAOQJF010000000
VERSION     DAOQJF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12350
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2546_length_12350_cov_5.774868"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLF85_04115"
     CDS             complement(<1..636)
                     /locus_tag="PLF85_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNE18873.1"
                     /translation="MTNQLTICEVGPRDGLQSAAQVLTVEQRVDFVAGLVGCGLSKIE
                     VGSLVNPKLVPTMANSDEVFERLCQKLPQNKENFSLLVPNEKGLNRALEIGCQSVAFF
                     TATSDTFNRKNINRSVAESIADLNPLIARAKAGGMLVRLYLSTVFDCAYEGTKTPEKD
                     LVPFLPFFDQVDEISLGDTTGRATQQQVAALIAYDLLKPWRNKIWWHFHDTY"
     gene            complement(633..1580)
                     /locus_tag="PLF85_04120"
     CDS             complement(633..1580)
                     /locus_tag="PLF85_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004508292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE18874.1"
                     /translation="MVTTIDCNYVMPEVAAAYLLHCGDKACFIDNNTNHCVPDLLKGL
                     VAAGYKPEQVEYIIITHVHLDHAGGTGLLLQQCTNAKVVAHPRAAPHVIDPSRLVKSA
                     QGVYGEENFRRLYGEIVPVPEDRVVIPADGETLKVGDTELTFIYTRGHANHHFVIVDK
                     KTKSVFTGDSFGLAYPMLQKGAYPFLFPSTSPTDFDAAEARISYDKILHCGATQAYPT
                     HFGVWKDIPAGYRMLSEYINYSEQIFNTLVAAPGNSPETDYKTAYDAYKQLFDKELTA
                     RGITLNTDEAKILELDLDLNAQGVAFAAQRARKKGKQAT"
     gene            complement(1628..2467)
                     /locus_tag="PLF85_04125"
     CDS             complement(1628..2467)
                     /locus_tag="PLF85_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18875.1"
                     /translation="MRIITEEAEMAKVAKIKSRKVARSEASALSLADQLFIASTLKYI
                     QPEDEFLPALAADRREAMSAWRAEHGAKLQSQKTDTAGRLIAGRFGRGVGMRWAARAA
                     VFITAVTGSLFLFTHVLDDTIRIEDELGKSAMGSEFGAQGRPVQLRVMRKRHKIGEIT
                     VADGSRLKVVKASNGDAFRSEMAFTGTEADFKLKYKGKASFIVNNGPFSAKVRIQSGN
                     ENDVHLKFRELGSAQPIGEPQFAIEVIKGDVQIAEIDDGDEFEHYKEGEKAVFSLDDS
                     ESL"
     gene            complement(2551..3087)
                     /locus_tag="PLF85_04130"
     CDS             complement(2551..3087)
                     /locus_tag="PLF85_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE18876.1"
                     /translation="MAAAADDEVNALFQAHQVQLARYLRNFVGEDEAQDVLQDVFVSF
                     LKQKRETKIRTQDELAWLYRACHNRAIDYIRRKKKVTHFTEEKFDRIEAPQENQHARA
                     WHALREQLHSLALGFDKKGEGALLLHLLEEGKPKLLIAETLDISDRHLRRKVAQLFQY
                     LQQELRKRGIEKLDLEGW"
     gene            complement(3105..3863)
                     /locus_tag="PLF85_04135"
     CDS             complement(3105..3863)
                     /locus_tag="PLF85_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNE18877.1"
                     /translation="MNLLEALLERDLAQNEQEARGLILAGKVLVNDVPVDKTGHRISA
                     SDEIRVRGLRNFVSRAGDKLQAALEAFTFPVAGRHFLDVGASTGGFTDALLRSGAASV
                     AAIDVGKGLLHNRLRTDNRVQVLEDCDFRKLSPEQLQHTPEAFVADVSFTSLLTMMEH
                     AFALIHEKARPREGIVLFKPQFELPRGEREMLQKGILTDEPRALRLIDEFGNALQPLG
                     IDIKARLASPVRGTKGNQEYLLHLQKPNRSLLND"
     gene            complement(3863..4708)
                     /locus_tag="PLF85_04140"
     CDS             complement(3863..4708)
                     /locus_tag="PLF85_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNE18878.1"
                     /translation="MQEILQQRKSRFERDFLSLLRSTSLKSAPAGLREATEYALSGSN
                     KRLRPVLVLEASRLAGLPEPRALLVAAAFECLHSYSLVHDDLPAMDDDDLRRGQPSCH
                     KKYGEATAILVGDLLQSLSFELLARAQMPAIALQYFARAVGAQGMVSGQFIDMQADTE
                     NTRKLHRLKTGKLIESAITLPYLARGEKLSLRVVRWGRGLGLLFQITDDLLDATADSA
                     TLGKTAGKDAEQHKKTFVAQLGLEETKAQAQQITARLKDSAKEIFPASELFRALPAYL
                     LNRRN"
     gene            complement(4755..5183)
                     /gene="ndk"
                     /locus_tag="PLF85_04145"
     CDS             complement(4755..5183)
                     /gene="ndk"
                     /locus_tag="PLF85_04145"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNE18879.1"
                     /translation="MFEKEFTLIMVKPDAVRSNYSGKILARIEEEGFKIRAMKRVRLS
                     LEQAQEFYKVHAARPFYGELCSFMASGPVVAAILERDNAVLHWRDVIGATDPAEAKEN
                     TIRKLFAKSKGENAVHGSDSVENAKIEAAFFFNGLELAGQ"
     gene            5274..5933
                     /locus_tag="PLF85_04150"
     CDS             5274..5933
                     /locus_tag="PLF85_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNE18880.1"
                     /translation="MKIKRFFLFLTVGYLVTSGYADGPSEFTLDKDKNGVKVYTRKIE
                     GSSLKEFKGVTAIKTSLTSLVALMDDTDALPKWLHNCGSAKLVQNINFAERITYTTVK
                     APWPVSDRDTVSYSKIVQDPKSKIITIYLKGMADRYPVQSGKVRVPSMKGFWQFIPNK
                     SGYVTIIYQLHSEPGGSLPDALANSTATDIPYHTLLNMHKIIKEEKYQNAKIPQVKEL
                     N"
     gene            complement(5930..6811)
                     /locus_tag="PLF85_04155"
     CDS             complement(5930..6811)
                     /locus_tag="PLF85_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE18881.1"
                     /translation="MLAELREWLSQGSHFFWKSHSIFYRSTFRKGKPAVLLIHGFPTA
                     SYDFAPIWQQLSKHFSLVTADMLGFGFSAKPYPYNYSVIEQADVFEALLKKLKVKDYH
                     VLAHDYGVSVAQELLARQVGKKRPVLSVCFLNGGLYPEYHKPLLVQKLMLSPLGPLLA
                     KFFSKAKLRASFDAIFGERKASEGELEAFWQLIEYNDGRRVIPNLLHYMNERRQYRER
                     WVNAMENAAAPIAMINGPMDPISGRHLAQAFAGRNPRARVEILEGVGHYPQVEAPEKV
                     AALYLSILGKRSFPKMD"
     gene            complement(6849..8012)
                     /gene="sucC"
                     /locus_tag="PLF85_04160"
     CDS             complement(6849..8012)
                     /gene="sucC"
                     /locus_tag="PLF85_04160"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNE18882.1"
                     /translation="MKIHEYQGKEIFRKYKIAVPNGVVTTSADEAAAIYTKLGKSVAV
                     VKAQIHAGGRGKGGGVKLAKSADEAKTNAKAILGMMLKTPQTGPEGQKVNSVLVEEGS
                     DILKEYYVGITLDRRISKPVMMVSTEGGMDIEEVAEKHPEKILKETIEPAIGLQRFQA
                     SRLAYGLGFDGDMAKQCIGILMNLWNCYDGEDCSLIEINPLISTKDGKVLALDAKVNF
                     DDNALYRHPDQAGMRDEAEEDPLEVEASKYNLNYVRLDGEVGCMVNGAGLAMGTMDII
                     KYAGASPANFLDVGGGANVTTVTNGFKIILSDPNVKAIFVNIFGGIVRCDRIANGIIE
                     AAKNVNINVPLIVRLAGTNAEEAKDILLKSGIKLEVADGLKDGAEKVVKAIRK"
     gene            complement(8076..8477)
                     /locus_tag="PLF85_04165"
     CDS             complement(8076..8477)
                     /locus_tag="PLF85_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE18883.1"
                     /translation="MSVLLDTHAWLWWLSSPELLPKKAARTIRESMESYKVLISSLSA
                     WEIAMLCSGGRLELATDYREFIHQTEKLPFVQFITPNNEILIGSVNLPAYEGKDPADR
                     IIVATANHMGATLVTKDEHMLAYRHVRTIWN"
     gene            complement(8474..8701)
                     /locus_tag="PLF85_04170"
     CDS             complement(8474..8701)
                     /locus_tag="PLF85_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18884.1"
                     /translation="MSGLTKTSFKAQALEHMRNVQKTKRPLIIMDRGKPVLKVVPYTA
                     DFDEELQKFRGLVLRYDDPTSPVGLEDWQLV"
     gene            complement(8810..10135)
                     /locus_tag="PLF85_04175"
     CDS             complement(8810..10135)
                     /locus_tag="PLF85_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18885.1"
                     /translation="MRKVGFLPLLFVASLLTNCTGELPLSDLINNTITLKVLGTYESN
                     DPYAWRNLAIDDVMQSGNPVTGASPAIAAGSNLVTYVTDGTTGLAVRPNDVKFYIDIA
                     EIRLARGQGKSSSQSISDYWSQFAIDRQLMCSDYSTAESGRILQNCSDQNGIQKLYDF
                     FNGGFTYPAVDVEHGVYNHLGIYFRRFVTSPAVRYSTDGTYTNSTTPEQTVTAAFDNR
                     TMYGFDIETLMQNNYGENSSEPRMFPLQRKDLNLQVYNDSEPYVLEIRVFVKNNMMVH
                     ILQPNDATNAAFTYVAPPDWNVNHAFTDSVNGIKQGGAMLMTARTYQPAKVGSIQFTA
                     ASTNGHYFAVMPAGTSLGTPYANTMPLAATRATNTVISNLPAGSYDVYKTCDLRRCTN
                     ASTAGSCDNLTGGTDNYPETYSLCTGGPYVVTAGTTTSVTTGNCTSLCP"
     gene            complement(10138..10716)
                     /locus_tag="PLF85_04180"
     CDS             complement(10138..10716)
                     /locus_tag="PLF85_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18886.1"
                     /translation="MLLGFVTLLPAMQALSAQVETEEWRFKPQLGIWFGPVTPVPGTR
                     ISDVLTTSLGGGMFFRMNIPSNTLRLEIGGSYSYYTSRTLSRLHSIPVYGALAYTIPV
                     NLPLTFQVKAGAGGNFLRNFPEGAKNWLPAGYLGFETSFPAGKLINIGVRADYYFVYE
                     QHLSPPANNPNYTVYNAHFLNFGLMVNFNLSR"
     gene            10897..11067
                     /locus_tag="PLF85_04185"
     CDS             10897..11067
                     /locus_tag="PLF85_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18887.1"
                     /translation="MQKPNTLLLILAIVILALFLPDGCIPFIVGTVWQVAKIVLILLA
                     IIIIIAYFRKKK"
     gene            11077..11700
                     /locus_tag="PLF85_04190"
     CDS             11077..11700
                     /locus_tag="PLF85_04190"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE18888.1"
                     /translation="MRAFRLLSIIVLLSSRLFAGEGLDYMQNIISNFEQLRGELAEDF
                     TPEKRKDVTHRARELALQILDVKEIGKLALGKHYAKLSPAQQEDFLGLFHELMANRVV
                     EANIPSQKVISGKIPIEIISERVETDDVFDKEAIIVNTKVPHRKSNFKVEFFLYRSDA
                     GLKLYDVHIDDASTLLDFRNQFSSIINKKGYKHLVNLLRERIRKINK"
     gene            11672..>12350
                     /locus_tag="PLF85_04195"
     CDS             11672..>12350
                     /locus_tag="PLF85_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE18889.1"
                     /translation="MNVFAKSTSKHLLALVALLLFRTTGIAAEKPIRFSEGLTQLLKN
                     NAKILGSKAQILSFEAIRTEASASDLPTLSFLSYVAPIYEVRGDATRSVDNFSKWGPF
                     LHGELQVVYPVFSFGRIKDAKQAASHAVEGGRFLHESQINKSIFEFKQLYMQCILLNR
                     LRSVLDDANDKMKTILGHAEKLYKKGTGEVQRKDLTRLRLFALELQRFNAEWHASKKT
                     AGLALGHF"
BASE COUNT         2594 a         3217 c         3534 g         3005 t
ORIGIN      
        1 gtacgtgtcg tgaaagtgcc accagatttt gttgcgccac ggcttcagca ggtcgtacgc
       61 aatgagtgca gctacctgct gctgcgtcgc gcggccggtc gtgtcgccga gcgagatctc
      121 gtcgacctgg tcaaagaatg gtaaaaaagg caccaggtct ttttcgggag ttttggtgcc
      181 ttcgtacgca caatcgaaca cggtcgaaag gtagagcctc acaagcatgc cgcctgcttt
      241 agcacgggca ataaggggat ttagatcggc tatcgattcg gccacgctgc ggttgatatt
      301 tttcctgttg aacgtatctg atgtcgccgt gaagaatgcg accgactggc aaccgatctc
      361 gagcgcgcgg ttaagcccct tttcgtttgg gaccagcagc gagaaatttt ccttgttctg
      421 cggcagcttc tggcacaggc gctcgaatac ctcgtccgag ttggccatgg ttggcacgag
      481 ttttgggttt accagtgaac cgacttcgat cttgctcagg ccacaaccca cgagtcccgc
      541 gacaaagtcg accctctgtt cgacagtaag tacctgtgct gccgactgca ggccgtcgcg
      601 cggcccgact tcgcagatgg tgagttgatt ggtcatgtgg cctgctttcc ttttttacgg
      661 gcgcgctgcg cggcaaacgc cacgccctgc gcgttcaggt cgagatcgag ttcgagaatt
      721 ttagcttcgt cagtgttgag cgttatgccc cgtgccgtga gttctttgtc aaagagttgc
      781 ttgtaggcat cgtatgcggt cttgtagtcg gtctccggtg agttgcccgg ggcggctacc
      841 agggtgttga aaatttgttc cgagtagttg atgtattcac tcagcattct gtatcccgcg
      901 ggaatatctt tccaaacgcc gaaatgcgtg gggtaggcct gcgtcgcgcc gcagtgcagt
      961 atcttgtcgt agctgatgcg cgcctcggca gcgtcaaaat ctgtcgggct ggtgctcggg
     1021 aacagaaaag ggtaagctcc cttttgcagc atggggtacg cgaggccgaa cgaatcgccg
     1081 gtgaatacgc ttttggtctt cttgtcgacg atcacgaagt ggtggttcgc gtgaccgcgc
     1141 gtgtagatga aagtaagctc ggtgtcgccg accttgaggg tttcgccgtc tgcgggtatg
     1201 acaacccggt cttcgggaac aggtacaatc tcaccataaa gacggcggaa attctcttcg
     1261 ccgtagacgc cctgcgcgct tttgaccagc cgtgaaggat caatgacgtg gggtgcagcc
     1321 cgcgggtgag ccacgacttt tgcattcgtg cactgctgca gtaacagccc ggtaccgcct
     1381 gcatggtcga ggtgcacgtg cgtgatgatg atgtactcaa cctgctcggg cttataacct
     1441 gctgccacga ggcccttcag caggtcgggc acgcagtggt tggtgttgtt gtcgatgaaa
     1501 cacgccttgt cgccgcagtg cagcaggtat gccgccgcaa cttctggcat gacgtagtta
     1561 cagtcgatgg tggtgaccat gggccactgc atggtgtttt cggcgggcga aaactacaat
     1621 cgatgcgtca gaggctttcg ctgtcgtcga gactgaagac ggccttttcg ccttctttgt
     1681 agtgctcaaa ctcgtcgccg tcgtcgatct cggcaatctg cacgtcgcct ttgatgactt
     1741 caatcgcaaa ctgcggttca ccgatcggtt gcgctgaacc aagctcgcgg aatttcaggt
     1801 gaacgtcgtt ttcgttacct gactgtatac ggactttcgc gctgaaagga ccgttgttca
     1861 caatgaacga cgctttgcct ttgtatttga gtttaaagtc ggcttcggtg ccggtaaagg
     1921 ccatttcaga ccggaaggca tctccgttgc tggccttgac taccttgagg cgagaaccgt
     1981 ctgctacggt gatttcgccg attttgtggc gcttgcgcat cacgcgcaac tggacggggc
     2041 gtccctgggc gccgaactcg ctgcccatgg cgcttttgcc gagttcgtct tcaatgcgta
     2101 tggtgtcgtc gagtacgtgg gtaaagagaa acagtgaacc ggtgactgcg gtaatgaaaa
     2161 ctgctgcgcg cgctgcccag cgcataccaa caccgcggcc aaaacgccct gctatcagcc
     2221 tgcctgcagt gtctgttttt tggctttgca gcttcgcgcc atgttctgcg cgccaggctg
     2281 acatcgcctc acggcggtct gccgcaagtg cgggcaggaa ttcatcttcg ggttggatat
     2341 atttcagcgt tgaggcgata aatagctggt cagccagcga cagcgcggac gcttcgctcc
     2401 gggcgacttt gcgggatttt attttggcga cttttgccat ctcagcctct tcagtaatga
     2461 tacgcaatta tcggcaaccg cggacaattt ttttccgcac aattacgaca gcattgcatt
     2521 atgtcgcata ggtggtctgc gcgctgtggg ttaccagcct tcgaggtcga gtttttctat
     2581 gccgcgtttg cggagttcct gctgcaggta ctggaacagt tgcgccacct tgcggcgcag
     2641 gtgccggtca gagatatcga gcgtttcggc aatcagcagt ttgggtttgc cttcttcgag
     2701 caggtgcagc agtagcgcgc cctcaccctt tttgtcgaag cccagagcca ggctatgcag
     2761 ctgttcgcgc agcgcatgcc aggcgcgggc atgctgattt tcctgcggtg cttcaatacg
     2821 atcaaatttt tcttccgtga agtgtgtcac cttcttcttg cggcgaatat agtcgatagc
     2881 gcggttatgg caggcacggt agagccacgc gagttcatcc tgggtgcgga ttttcgtttc
     2941 gcgcttctgc ttcaggaacg agacaaagac atcctgcagg acatcctgcg cctcgtcttc
     3001 accaacgaaa ttgcgcaggt agcgggccag ctgcacctgg tgcgcctgga agagcgcgtt
     3061 tacttcatcg tcagccgcag cggccataag ctcaagccag aggttcagtc attgagtaaa
     3121 gaccgattgg gcttctgcag gtgtagcagg tattcctggt tgcccttggt gcccctcacc
     3181 ggcgaggcca ggcgggcctt tatgtcgata cccagtggtt gcagtgcgtt gccaaattcg
     3241 tcgatcaggc ggagtgccct cggttcatca gtcaatattc ccttttgcag catttcgcgt
     3301 tcgccgcgcg gcagttcaaa ctgcggtttg aagagtacga tgccctcgcg cgggcgggct
     3361 ttttcgtgga taagcgcaaa tgcatgttcc atcatggtca gcagcgatgt gaaggagaca
     3421 tccgccacaa acgcctcggg ggtatgctgt agctgttcag gagaaagttt gcggaaatca
     3481 cagtcttcga gcacctgcac ccggttgtct gtgcgcaggc gattgtgcag caggcccttg
     3541 ccgacgtcga tggctgcgac agaggcggcg cccgaccgca acagggcgtc tgtgaagcct
     3601 ccggtcgaag ctcccacatc gagaaaatgc ctgcccgcaa cgggaaaggt aaacgcctcg
     3661 agtgccgcct gcaatttgtc acccgcgcgc gaaacaaaat tgcgcaggcc gcgcacacga
     3721 atctcgtcgc tggcagaaat tctgtgaccc gttttgtcga caggcacatc gttcacaagc
     3781 accttgccgg ccaggatcag cccgcgcgct tcctgttcgt tttgagcgag gtcgcgttcg
     3841 agcagcgctt cgagtaggtt catcagttgc ggcggttcag caggtaggcg ggtagtgcgc
     3901 gaaagagttc agatgcaggg aaaatttctt ttgcactgtc tttgagccgc gcagtaatct
     3961 gctgcgcctg cgctttggtt tcttcgagac cgagttgcgc gacgaaagtt ttcttgtgct
     4021 gttcggcgtc tttgcccgca gtcttgccga gcgtcgccga atcggcagtg gcatcgagca
     4081 ggtcgtcggt gatctggaac aacagaccga ggccgcgtcc ccagcgcacg acgcgcaggc
     4141 tcagtttttc gccgcgcgca aggtaaggca gtgtaatcgc tgattcaatc agtttgcctg
     4201 tcttcaggcg gtggagtttc cgcgtgtttt cggtgtcagc ctgcatgtcg atgaactgtc
     4261 ccgacaccat accctgcgct ccaacggcgc gcgcaaaata ctgtagtgcg attgctggca
     4321 tctgcgcgcg tgcgagcagt tcaaagctca ggctctgcag caggtcgccc accagaatgg
     4381 cagttgcctc tccgtatttc ttgtggcacg aaggctggcc ccggcgcagg tcgtcgtcat
     4441 ccattgcagg cagatcgtcg tgcacgagcg aataactgtg caggcactcg aacgcggcgg
     4501 caaccaggag cgcacgcggt tcgggcagac ctgcgaggcg cgatgcctcg agcacaagca
     4561 ccggccgcag gcgcttattg ctgcccgaga gcgcatattc agtcgcttcg cgcaggccgg
     4621 caggtgctga cttgagcgac gtcgagcgca gaagagataa aaaatcccgc tcgaagcggg
     4681 atttccgttg ctgcaagatt tcctgcatgg ctgtacttgg ttgcgcagcg gtgctgcgcg
     4741 atcaagccgt tagcttattg gcccgcgagt tcgaggccgt tgaagaaaaa ggcggcttcg
     4801 atcttagcgt tttctacgga gtctgaaccg tgtacggcgt tttcgccttt tgatttggca
     4861 aagagcttcc ggatagtgtt ttctttcgct tcagccgggt cggttgcacc gatgacgtcg
     4921 cgccagtgca gtactgcgtt gtcgcgttcg agtattgccg ctactacagg gcctgaagcc
     4981 atgaagctgc aaagttcgcc gtagaaaggg cgcgctgcgt gcactttgta aaattcctgc
     5041 gcctgttcga ggctgaggcg tacgcgcttc atcgcgcgga ttttgaagcc ctcttcttca
     5101 atgcgggcga gtattttgcc cgagtagttc gagcggaccg cgtcgggctt taccataata
     5161 agggtgaatt ctttttcaaa catagggtaa aatgggttta tgagacaccg cgtcaaacgt
     5221 atggagaccc aaatggcttt gacaagcagg gccgccgggc atatttggga cttatgaaaa
     5281 ttaaacgatt tttcctgttt ttaacggttg gctaccttgt caccagtggt tatgccgatg
     5341 gcccttcaga atttactctc gataaagata aaaacggcgt aaaggtgtac acgcgcaaga
     5401 tcgaaggttc ttccctgaag gagttcaagg gtgtgacagc gatcaagacc tcgctgacca
     5461 gcctggtcgc cctcatggac gataccgacg ccctgccgaa atggctgcac aactgcggtt
     5521 ctgcgaagct ggtccaaaat atcaactttg ccgagcggat tacctacacc actgtaaaag
     5581 ctccgtggcc ggtgtcagac cgcgatacgg tttcttacag caagatcgtg caggatccca
     5641 agagcaagat tatcaccatc tacctcaaag gtatggcaga tcgttaccct gtacagagtg
     5701 gcaaagtacg tgtaccctct atgaaaggtt tctggcagtt tattcccaac aagagcggct
     5761 acgtgactat catctaccag ctgcactcag aacccggtgg atctctaccc gatgcgctgg
     5821 caaactctac cgcgacagat atcccttacc acacgctgct gaacatgcac aagatcatca
     5881 aagaagaaaa ataccagaac gcgaagattc cccaggtaaa agaactgaac taatccattt
     5941 tggggaagct ccgcttcccc aaaatggata aatagagcgc ggccaccttc tccggcgctt
     6001 ccacctgcgg gtagtggccg acgccttcga gaatttctac ccgcgcccgc gggttccgcc
     6061 cggcaaaggc ctgcgccagg tgcctgcccg agatcgggtc catcggaccg ttaatcatcg
     6121 ctatgggtgc cgcggcattc tccatggcgt ttacccagcg ctcgcggtac tgccggcgtt
     6181 cattcatgta gtgcaaaagg tttggaatca cgcggcggcc atcgttgtat tcgatcaact
     6241 gccagaaagc ttcgagctcc ccctcgctgg ctttgcgctc gccgaagata gcatcgaacg
     6301 aagcgcgtag ctttgccttg ctgaaaaatt ttgccagcag tggccccagg ggtgaaagca
     6361 tcagcttctg cacgagcagt ggcttgtggt attcggggta cagcccgccg ttcagaaaac
     6421 agacagatag aacgggtcgt ttcttaccaa cctggcgtgc gagcagttcc tgcgcgactg
     6481 agacaccgta gtcgtgggca agtacgtggt aatctttcac ctttaacttc ttgagcaatg
     6541 cttcgaatac atctgcctgc tcaatcaccg agtagttata cggatagggc tttgccgaaa
     6601 agccaaagcc gagcatgtct gcggtcacga gggagaagtg tttcgagagt tgctgccaga
     6661 tcggagcgaa atcatacgac gccgtcggaa aaccgtggat caacagaacc gcaggcttgc
     6721 ctttcctgaa agtgctgcgg tagaagatgg agtgactttt ccaaaaaaaa tggctacctt
     6781 gcgatagcca ttcccttagt tctgccagca tgttggtttc ccttccttta ccggaaggac
     6841 aggctgggtt atttccggat cgccttaacg actttctcgg cgccgtcttt gaggccatct
     6901 gctacttcga gtttgatacc gctcttgagc agaatatctt tcgcttcttc ggcgttggta
     6961 ccggcgaggc gcacgatcag cggcacgttg atgttcacgt ttttcgctgc ttcgatgatg
     7021 ccgttcgcga tacgatcgca gcgcacgatg ccgccgaaga tgttcacgaa gatggccttc
     7081 acgttcgggt cagaaagaat gatcttgaag ccgttggtaa ccgtagtcac gttcgctcca
     7141 ccgccgacgt cgaggaagtt tgccggcgat gcaccagcgt acttgatgat gtccatggta
     7201 cccatcgcga gacctgcacc gttcaccatg caaccgactt cgccgtcgag gcgcacgtag
     7261 ttgaggttgt acttcgaagc ttccacttcg agcggatctt cttccgcttc atcgcgcatg
     7321 cctgcctggt ccgggtggcg gtagagcgcg ttgtcgtcaa agtttacttt cgcatcgagt
     7381 gcgagcacct tgccgtcttt cgttgagatc aggggattaa tttcaatgag cgagcagtct
     7441 tcgccgtcgt agcagttcca caggttcatc aggatgccga tgcactgctt ggccatgtcg
     7501 ccgtcgaaac cgaggccgta cgcgaggcgt gacgcctgga agcgctgcag gccgatagcg
     7561 ggttcgatgg tttctttgag gattttctcg gggtgctttt ctgcgacctc ttcaatatcc
     7621 ataccgcctt cggtagagac catcattacc ggcttcgaga tgcggcggtc gagcgtgatg
     7681 ccgacgtagt attctttgag gatgtctgag ccctcttcaa ccagtaccga gttgaccttc
     7741 tggccttctg gcccggtttg cggggttttg agcatcatac ccagaattgc cttcgcgttg
     7801 gtctttgctt cgtcggcaga ttttgcgagt tttaccccgc cgcccttgcc gcggccacct
     7861 gcgtggatct gtgctttgac gactgcaact gacttgccca gtttcgtata gatagcggcg
     7921 gcttcgtcag ccgacgtggt aacaacaccg ttgggcacgg cgatcttgta cttgcggaat
     7981 atttctttgc cctggtattc gtggatcttc atggtggatt agagccgacg agtcaaacag
     8041 tgggtcaaga agaaggcgta atagcttagc gtgatttaat tccagatcgt ccgcacgtgg
     8101 cgataggcca gcatgtgttc atctttagtg acaagggtag cacccatgtg gttcgccgtg
     8161 gccacaatga tgcggtcggc cgggtccttg ccttcgtagg ctggcaaatt tacagaaccg
     8221 atcaggatct cattgttggg ggttatgaat tgcacgaacg gcaatttctc tgtctggtga
     8281 atgaattccc gataatcggt ggcgagctcc agccggcccc cgctgcataa catggcgatt
     8341 tcccatgcgc tcaaggaaga gatcagcacc ttgtagcttt ccatggactc gcgtatggtt
     8401 ctggcggcct ttttgggcag taattcgggg ctgctcaacc accagagcca ggcgtgtgta
     8461 tcgagcagta cgctcatacg agctgccagt cttcaaggcc aactggtgac gtcgggtcgt
     8521 cgtagcgtaa gaccagacca cggaattttt gcagctcttc atcgaagtcg gcggtgtaag
     8581 gtacgacctt gagcacgggt ttcccgcgat ccatgatgat cagtggacgc ttggttttct
     8641 gcacattacg catgtgttcc agcgcctgag ccttgaagga tgtcttagtt aagcccgaca
     8701 tgaccataaa tatatggtca tgtcggtggg acgacaagtt ttcaatggca gagcgacagg
     8761 ggcagtttta tggcttagat ggccaccgaa ttcattcggt ggctaggggt tatgggcaca
     8821 aagaagtgca gtttccagtt gtgacagatg tggttgttcc ggcagtcaca acatatgggc
     8881 cgccggtgca taacgaatag gtttccgggt aattgtcggt cccgccggtc aggttatcac
     8941 agctgcccgc tgtgcttgca ttggtacaac ggcgtaaatc gcaagtcttg taaacgtcat
     9001 agcttccggc cggcagattg ctaataacgg tgttcgttgc gcgtgttgca gcgagcggca
     9061 tagtgttggc gtaaggagtg ccaagggatg ttcctgctgg catcacggca aaataatggc
     9121 cattggtact tgctgccgta aattgaatcg atcccacctt cgctggctgg tacgtccgcg
     9181 cagtcatcag catcgcgccg ccctgcttga tgccgttcac cgaatcggtg aaggcgtggt
     9241 tcacgttcca gtcgggcggg gcgacgtagg taaaggcggc gttggtagca tcgttaggtt
     9301 gcagaatgtg caccatcatg ttattcttca cgaagacacg gatttcgagc acatacggtt
     9361 cgctatcatt gtagacctgc aggttcaggt ctttgcgctg cagcgggaac atgcgtggct
     9421 cgctcgagtt ttcgccatag ttgttctgca tcagcgtttc gatgtcaaag ccatacatcg
     9481 tacggttgtc gaaggcggcc gtgactgtct gttcgggtgt ggtggagttt gtgtatgttc
     9541 cgtcggttga gtagcggact gctggtgagg taacgaagcg ccggaagtag atacccaggt
     9601 ggttgtacac accgtgctct acgtcgaccg cgggataagt gaaaccaccg ttgaaaaagt
     9661 cgtagagttt ctggatgccg ttttggtcag aacagttttg cagtattcgc ccgctctcgg
     9721 cagtactgta gtcagagcac atgagctggc gatcgatggc gaattgcgac cagtagtcag
     9781 aaattgactg gctcgaagac ttgccctgac cccgcgcaag gcgaatctct gcaatgtcga
     9841 tgtagaactt cacgtcgttg ggacgaactg ccagaccagt tgtgccgtcg gtaacgtagg
     9901 tgacaagatt ggaaccagct gctatggcag gcgaggctcc agtcaccggg ttgccggatt
     9961 gcatgacgtc atcgatggcg aggtttcgcc acgcataagg atcattgctc tcgtacgtgc
    10021 ccagcacctt cagggtgatg gtattattga tgaggtcaga gagcggtaac tcgccggtgc
    10081 aatttgtcag cagcgaggca acaaacagaa gaggtagaaa tcctactttt ctcatgatta
    10141 cctgctcagg ttaaaattga ccattaggcc gaagttgaga aagtgcgcgt tgtaaacggt
    10201 gtagttgggg ttatttgccg gcgggctgag gtgctgttcg tagacgaagt aataatcggc
    10261 gcgcacaccg atgttgatga gcttgcccgc aggaaacgaa gtctcaaagc ccaggtaacc
    10321 agcgggtagc cagttctttg ccccttcggg aaaattgcgc aggaagttac cgccggcccc
    10381 ggcctttacc tggaatgtca gcggcaggtt tacgggaatt gtgtaagcga gtgcgccata
    10441 gacaggtatt gaatgcaggc gcgagagtgt gcgcgaggtg tagtagctgt aagacccacc
    10501 aatttcgagg cgcagtgtgt tcgagggaat gttcatgcga aaaaacattc cgccgccgag
    10561 gcttgtcgtc agcacatccg aaattcttgt gccaggcaca ggcgtgactg ggccgaacca
    10621 gatgccgagc tgcggcttga agcgccattc ttcggtttcg acctgtgcgg aaagcgcctg
    10681 cattgccggc agcagtgtga cgaagccgag cagcatagcg cgtcgggtat atataaagaa
    10741 gtgattccgc atctcgtctt ttatggcttt aagggagttt tagacttagc gtcaaggcaa
    10801 agcccaaaga ggcccttggc attgtactgc tttgcctatt gtactcttgg tgacatttaa
    10861 cttgacggca agcgttggtt tgcggaaaaa acggccatgc aaaaaccgaa tacgttgctg
    10921 ctcattctgg cgattgtcat tctcgcgcta tttctgcccg acggatgtat tccgttcatt
    10981 gtcggtactg tctggcaagt ggcgaaaatt gtcttaatcc tgctggcaat cattatcatt
    11041 attgcctatt ttcggaaaaa gaagtagcat aagctaatgc gggcctttcg tcttttatca
    11101 attatagtcc tgctctccag ccggcttttt gctggcgagg gactcgacta catgcagaac
    11161 atcatcagca actttgagca gctgcgtggc gagctggccg aggactttac gcccgaaaag
    11221 cgcaaagatg tgacgcaccg ggctcgcgag ctggcgctgc agatactcga tgtgaaagaa
    11281 atcggtaagc tggcgctggg taagcactac gcaaaactca gcccggcgca gcaagaagac
    11341 tttctcgggt tgttccacga actcatggcc aaccgcgtcg tcgaggcaaa cattccgtcg
    11401 caaaaagtca tttccggaaa aattccgatc gagatcattt ctgaacgcgt cgaaacagat
    11461 gacgtctttg ataaagaagc gatcatcgtg aacacgaaag tcccccaccg aaaaagtaat
    11521 ttcaaggtgg agttttttct ctacaggtca gatgccggcc tgaaacttta tgacgtccat
    11581 atcgatgacg cttcaacgtt gctcgatttc cgtaaccagt tctcttcgat catcaacaaa
    11641 aaaggctata agcaccttgt gaacctgctg cgtgaacgta ttcgcaaaat caacaagtaa
    11701 gcaccttctt gccctggtgg cgttgctgct tttccggacg accggaattg cagcagaaaa
    11761 gcccatccgc ttcagcgaag gcctgacgca gctgctcaag aacaatgcga agatactggg
    11821 aagcaaagcg caaatactga gctttgaagc tattcgcaca gaagcttcag cgagtgactt
    11881 gccgacgctt tcatttctga gttatgtggc gcccatctat gaagtgcgcg gtgatgcgac
    11941 ccgctcggtt gacaatttct ctaaatgggg tcctttcctg cacggcgagc tgcaggtggt
    12001 ctacccggtg tttagttttg ggcgcatcaa ggacgcgaag caggcagcat cgcacgcggt
    12061 cgagggcggc cgctttttgc acgaaagcca gatcaataag agtatctttg agtttaaaca
    12121 gctctatatg cagtgtatac tgctcaaccg cctgcgttcg gtgctcgatg atgcgaacga
    12181 caagatgaag acgattcttg gtcacgcgga gaagctctac aagaaaggta caggcgaagt
    12241 gcagcgcaaa gacctgactc gcctgcggct ctttgctctc gagctgcagc gctttaatgc
    12301 cgagtggcac gccagcaaaa agactgccgg gctggcactt gggcatttcc
//
LOCUS       DAOQJF010000049        12348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2548_length_12348_cov_5.111527, whole genome shotgun sequence.
ACCESSION   DAOQJF010000049 DAOQJF010000000
VERSION     DAOQJF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12348
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2548_length_12348_cov_5.111527"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLF85_04200"
     CDS             complement(<1..786)
                     /locus_tag="PLF85_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE18890.1"
                     /translation="MAVSAEVTAFLEDLGENLGIIENALVRLEDSPGNQEYLHEIFRA
                     AHTIKGNAAMMNLGNLVALGHALETALQEVLAGNLVASQHTVGVFHECRAAMLMIADA
                     LRSGSDGSQVSIRPLTDQIQVLLLSTTPAQESATGRTVTVTLNISTAEQAPSVRAFLA
                     ETRLSEFGTIIRKEPADDFLETPQFAASDRKLTFLLQTAAETDEIRANLNIDLVENVQ
                     VSESASRATSSAEPLRSPRETAGGESGSARTAAPQDIATSTADT"
     gene            887..1891
                     /locus_tag="PLF85_04205"
     CDS             887..1891
                     /locus_tag="PLF85_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18891.1"
                     /translation="MVPQAAESETPVQGRGIHALKGDKVHVRSFTFSFALGVFLLVEL
                     ILLSSLTAFSFVSLRETARQLEMGYSERGREIALSVSTGAALAEQKVIARLSAIFAGI
                     VNKNQYLEGERPITEIFILRKDGRVLAHSDASMVTIGTREAANEISAKYNNEFYHSSL
                     YYEANSVQQQNLEKPEGIFHGKNSFIVDSLMSSDVHYSTDFSIALKEKDPKSGKEKSY
                     ATLHVIMNRLGEYYYLAAILKKYLLLFAMVFLAGFLVTGIILFAFWLRARTIQREWKS
                     ALTYMWENEVIKTEVTHGFQALSGKLNEIEKKVNQPAALPQARPQADILDAILIEPAK
                     "
     gene            1910..2791
                     /locus_tag="PLF85_04210"
     CDS             1910..2791
                     /locus_tag="PLF85_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE18892.1"
                     /translation="MLEFNTALEKDVLVIELEGALDSHSAADFRRYFQDKTGAGYRAF
                     ALDCLCLEYISSAGIAAIIDLQSQLQAQGGKMVLFQLATETRQLLRFLKLEDKLTIVS
                     EYDDAVAALTGYRRVIPEADIVAPEELRIIDQNGPEPVAEETKPMAEVTPAPAEEPAA
                     TAVNTPVMPLVPQGNEMLMEEHFAAEKKGLHPVHAETEAITAEAPPTAVSTQPAQPAA
                     EEAPTVLPPGPATQHVAATTLPQAVTMPEAQELKLNTGAKRLISCPNCKSVLRVAVAG
                     DYLCPACRFRFSFKASA"
     gene            complement(2808..3953)
                     /locus_tag="PLF85_04215"
     CDS             complement(2808..3953)
                     /locus_tag="PLF85_04215"
                     /inference="COORDINATES: protein motif:HMM:NF027399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNE18893.1"
                     /translation="MSKLLDSAKAGQAKFFLQFGGQGAPYLKEMQKLYAEASMKPYFD
                     VAVSAIEAAAKMVEGTAAHPEKVDFRKWLTEPDHAPSDDAMSIAGISLGLIQATQFAH
                     YEYLHQQGFDRAEMLKHTVAASGHSQGLIAASFTALGLEGKAYYDAVYKYIQYLFLMG
                     VRAQEVFPHIHATADEAAKSEALGAKGPAPMVAVLGESHATIEAWVKEFNGAAKKLFV
                     SLYNTPNNRIISGARADLIAFHERYKAEFEAKQIKFVYLRSTCPFHCELMIPILEKFK
                     PDLAKIGFDFKGSDLKFPVYSFWNGDNLQNEAELGMRMTDDLMVKTLYWEKSMLPVKN
                     DKSITAILDFGPGKTSQRLSMDTLAGLGHEVPVYAIAFAKDLKTVLE"
     gene            complement(4049..5056)
                     /locus_tag="PLF85_04220"
     CDS             complement(4049..5056)
                     /locus_tag="PLF85_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18894.1"
                     /translation="MHLYGKLVLVFLLAASGLVASNSSESSFQLAPAPIPYPYFEPGA
                     TDGKIDFSYLRLDFDQYSFNGMSAFGKARKAFTDSIAIDGMAGLMYLSGQLPGVGPIS
                     PLPAYAANGSFLGYYTPIPAKNGNASILSLAFSANLELQVVRSPLFNAIIFAGPSLNV
                     TSLTLKTLYNKYYVPTGTTYTGFTDTLTTSIMLGGIQAGMQIDIPLGSMIRMSPFFVI
                     SSMSGSATITDDPGTKTTNAYSASFEVPASTTTSLGFDIFINEISIGAMAQSGKSTQT
                     GSNSSYLQLSVGYVFSSRDQAAEKAADAKPDEEATEETAKREPAEKKPAAKKAPAPKT
                     R"
     gene            5217..6110
                     /locus_tag="PLF85_04225"
     CDS             5217..6110
                     /locus_tag="PLF85_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNE18895.1"
                     /translation="MLRQIASSRFFQNFITVVIVLASMLVGIETYPEIEKQYHKLLHT
                     LDLLIIGIFTLEVVIKIGAEGRHPLRYFRDPWNVFDFVIVTACYLPFENQYITVLRLI
                     RLLRVLRLLRAFPKLQILVGAIFKSIPSMGYVSLLMGLLFYVYAIAGVLLFGKNDPVH
                     FGNLNSSLLSLFQTVTLEGWVELMNIQLHGCDKIGYEAFRELCTQPARSYVAPLFFIS
                     FILVGTFVLLNLFIGVILTGMEEARNELADLKMKESSGSPRSYDLQKIQAEIAKLNDA
                     LKTLKVIRVKYRDKTGEDADG"
     gene            6103..6681
                     /locus_tag="PLF85_04230"
     CDS             6103..6681
                     /locus_tag="PLF85_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18896.1"
                     /translation="MAKTWFNIQSNLTFTGKGALLEVENIIGNVTFAQGISKAIGLFQ
                     NIDREYKKAVGNDGKIVGKERTDILIQVDRFLNGLILVWKKLEMRNDNMIIIENKNVN
                     FLMTINERNGLWEASGTVPHFFVKPVKNFQEIYNNKLTPEIVALLKRYAEVVEDGIID
                     DDEREDLKAGMQRVLYYTFFLRTQVEKLLIHD"
     gene            6921..7238
                     /gene="trxA"
                     /locus_tag="PLF85_04235"
     CDS             6921..7238
                     /gene="trxA"
                     /locus_tag="PLF85_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNE18897.1"
                     /translation="MKTAAAPNKFAEFVSSSDTPVLVDFYADWCGPCHMMAPILNDLK
                     STWGDRIRIIKVDTELQPQIAAQYQISGIPTLILFKEGKPVHRTSGVMPLQRLVQEYG
                     KYL"
     gene            complement(7243..9996)
                     /locus_tag="PLF85_04240"
     CDS             complement(7243..9996)
                     /locus_tag="PLF85_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE18898.1"
                     /translation="MRRPDRHPERWPAKFRLLAACLLLLLASPFCPTLADRPTADYGH
                     LSLRPFHQALAGQMTQSMPVQAGAAVNPALLGYGEQNTFFAGGIFGQGLTGFYADGAL
                     FSPFGGLMLSAEYLQARDKTASLSFGYGSFLSRRVATGLSITPRYTTGDASNAFGLGL
                     DPALLFDSKWHTSFAGNDGWGLYSPSVFFRTQNLAIPVGETSLLASPSAHVGFLTGMY
                     QSTSFNLALITSTYGTERFDKLPLLVGVQAQYRFAMLSFGYGASNFRQVGNGFHLGLG
                     AVLPADFGDTYLFYAVGLANSERSDLHSLTLGVRLGGVDRDAPEVSVETEGDYFSPNN
                     DGVQDLVVFNPRVRDKSPIVFYEFRVLDSKGNLVYRQRSDERIREKDFSWSLFVRSFV
                     APRARSDIPERLTWNGRVQTEQKKPVKDAVFEEETPDRALPDGAYTWDFRAIDEKNNE
                     SAPVSGQVHIDTKPPAASVELGDDLISPNGDGRRDLLVITQDTTPADRYEGTIVNAQG
                     IKIRSWKWNENAPTRFDFDGRMDNDELAEEGVYRYRLLGHDAAGNSVHAESGNFYISR
                     RVDEVFLRSSALSLNPKNPKLASISFNPSVAFGDGYKEGEILISRQCGPKKEDFVHRI
                     AVTTVKVDDAKKQPRKDKQPVYTWAGESPGGKPAPDGNYCALFRARYENGNGPESPAL
                     KLSLDSTGPEAEITADLTMRQFAPDGDGDNEEQAFRIVWHDQSPIDSYQLTISEVAPE
                     ANGVRLIPVRRFSGQGEIPQTIYWDGKTESGALIESLTQYEYTLTATDTLGNSTTTAP
                     RRFESGALAVATTTGFLIRLPYANLEDPVTDRMQTVYRLLDRYPKYRVKIEAHTALKG
                     KIEQNLSLSESAARKLRDFFVEKGVTPDRITYQGFGDTAPLYSAKHIFAGKNRRFDIY
                     LTR"
     gene            10081..10656
                     /locus_tag="PLF85_04245"
     CDS             10081..10656
                     /locus_tag="PLF85_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18899.1"
                     /translation="MQDIDFSRYYRKGRRTYAASEAPEVRKTGAPVNKLSLYLAIAFS
                     SLAIGFIGGTQLQKNKMMRELENRVAPESAQNGTMVTANVAAGPAPEAEKPPETIRTT
                     RPDEGARAVRPDKGLEGSFLILAKIYPDEKEASLAGLNLKRNGMKAFMARNGKKVKLY
                     VGPIEGKNSAYEALAQLKKNPEFTGAILYRK"
     gene            10698..11180
                     /locus_tag="PLF85_04250"
     CDS             10698..11180
                     /locus_tag="PLF85_04250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE18900.1"
                     /translation="MPSAINPQIRDARPGDEDAIAALLEPYVLKKIVLRRSPAEIREN
                     LGQTIVYEENGRLLGTTSLVFFTDTLCEIRALVIADELQGKGVGRQLVLAAEEKALQH
                     SAQRPLRFFALTYTPEFFERCGYERTTKDKFPEKIYEVCNFCLRKDDCHEIAVQKTTN
                     "
     gene            11381..>12348
                     /locus_tag="PLF85_04255"
     CDS             11381..>12348
                     /locus_tag="PLF85_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18901.1"
                     /translation="MELFRKVRPLLVIGAAVTLAIFATIYLFRFFNRANIEKALAGKD
                     GFFVSFVVTSANDDKKVQVVSVAQIFPATQRIGVISFYPAIRLKDNEPSLEEMYKSGG
                     VAAINSAVSEFLGERVGMHVKLRDTALAHFVDVLGGIDYFLPSGDLLKDENLPKGQFI
                     LDGSLVPRLLNPPEKNEYTPAFKLFRYYSLMLNAWNQRSRLWPVIKDEKSFALAVRNV
                     ETNASAHDLFVLAQTFATQKSWVPILLEVPVRRVKDAFYVDKDSAALYFRNLTKQLTQ
                     KEHPFINEPPKMEVKNGTHVPNLARTMRAELSRKGVQILEFTNADH"
BASE COUNT         2745 a         3369 c         3487 g         2747 t
ORIGIN      
        1 agtatctgcg gtactggttg cgatatcctg tggggcggca gtcctggctg aacccgattc
       61 gccacccgcc gtctcgcgag ggctgcggag gggttctgct gagctggttg cgcgcgatgc
      121 agactcgctg acctggacat tctccaccag gtcgatgttc aggttagccc tgatctcatc
      181 cgtttctgca gctgtctgca acaggaaggt gagcttgcgg tccgatgcgg cgaactgtgg
      241 tgtctcgaga aaatcatctg caggctcttt gcgaatgatc gtaccgaatt cgctcaacct
      301 ggtttcggca agaaaggcgc gtacagaagg cgcctgttct gcggtggaaa tattgagcgt
      361 cacggtgacc gtgcgccctg tagctgactc ctgcgcgggc gtcgtcgaga gtaacagaac
      421 ctggatctgg tcggtaagcg ggcgtatcga aacctggctg ccgtcgcttc cgctgcgcag
      481 cgcatcggca atcatcagca ttgccgcacg acactcgtga aagacaccca cggtatgctg
      541 tgatgccacg aggttgccgg ccaggacttc ctgcagcgcg gtctcgagtg cgtggccaag
      601 ggccaccagg ttgcccaggt tcatcatcgc ggcgttgccc ttgatggtgt gcgcggcccg
      661 gaaaatttcg tgcaggtatt cctggttgcc cgggctgtcc tctagcctga ccagcgcatt
      721 ctcgatgatt cccaggtttt cgcctaaatc ttccaaaaat gcggttactt ccgcgctgac
      781 ggccatgtgc tattttcggc aataaaaccg ggttgcccag ccgaaaaatg ctgccggtta
      841 agaagaaatt tctgtaaggt tacgcagtaa tgaccgataa cagactatgg ttccacaggc
      901 cgctgaatct gaaacacccg tgcagggcag gggtattcac gcactgaagg gcgacaaagt
      961 ccacgtgcgc agctttacct tcagttttgc gctgggcgtc tttctgctcg tggagcttat
     1021 tctgctgtcg tcgctgaccg ccttcagctt tgtttcgctg cgcgaaaccg cgcggcagct
     1081 cgagatgggc tattctgagc gcggtcgcga aattgccttg tctgtctcaa ccggtgcagc
     1141 gctggcggaa caaaaggtaa tcgcccgttt gagcgcgata tttgccggaa ttgtcaacaa
     1201 gaaccagtac cttgaaggcg aacgccccat taccgaaatc tttatcctga gaaaagacgg
     1261 gcgcgtgctt gcgcattctg acgcatcgat ggtgaccatc ggcacacgtg aagccgctaa
     1321 tgaaatttca gcaaaatata acaacgaatt ctaccattcg tcgctctact acgaggcgaa
     1381 cagtgtgcag cagcagaacc tggaaaagcc tgaagggata ttccacggta agaactcgtt
     1441 cattgttgat agcctgatga gctctgacgt gcactattct accgacttta gtattgcttt
     1501 gaaagaaaaa gatccaaagt cgggcaaaga gaaatcatac gcgaccctgc acgtcatcat
     1561 gaaccgcctc ggcgagtact actacctggc cgcgatcctg aaaaaatacc tgctcctctt
     1621 tgccatggta tttctcgcag ggttcctggt gacgggaatc atcctgttcg ctttctggct
     1681 gcgtgcccgt accatacagc gcgaatggaa aagcgcgctg acctacatgt gggaaaacga
     1741 ggtcatcaag accgaggtga cgcatggctt ccaggcgctg agcggcaaac tcaacgaaat
     1801 tgaaaagaag gtcaaccagc cggccgccct gccacaggcg cgaccgcagg ccgacatact
     1861 cgacgcgata cttatagaac ccgcaaaata atggtggcac caggctaaaa tgctcgaatt
     1921 caatacagcg ctagaaaaag atgtgttagt catagagctc gagggagctc tcgactcgca
     1981 cagcgccgcc gacttccgcc gttatttcca ggataagacg ggcgctggct accgtgcatt
     2041 tgcgctcgac tgcctctgcc tcgaatacat ctcttctgca ggcatcgccg ccatcattga
     2101 tttgcagtcg caactgcagg cgcagggcgg caagatggtc ctgtttcaac tggccaccga
     2161 gacaaggcag ctgctgcgtt tcctgaagct agaagacaag ctcacgatcg tgagcgagta
     2221 cgatgacgcg gttgcagcgc ttaccggtta tcgccgcgtg ataccagaag cagatattgt
     2281 tgcgcccgaa gagttgcgca tcatcgacca gaacggcccg gagcctgtcg ctgaagaaac
     2341 gaagcccatg gctgaagtta cgccggcgcc cgccgaagaa ccagcagcca ccgcggtaaa
     2401 tacgccagtg atgccactcg tgccccaggg taacgagatg ctgatggaag agcattttgc
     2461 cgcagaaaaa aaaggactgc accccgttca cgcagagaca gaagccataa ccgcagaagc
     2521 tcccccgacg gcggtttcga cacaaccagc gcagccagcc gcagaagaag cacccacggt
     2581 tttgccaccg ggccctgcaa cgcagcatgt ggctgcgaca accttaccac aggctgtgac
     2641 catgcctgaa gcgcaggagc tgaagctcaa cacgggtgcg aagcgcctga tcagctgccc
     2701 gaactgtaag agtgtcttga gggttgcggt cgcgggcgat tacctctgcc ccgcgtgccg
     2761 ctttcgtttt tcttttaagg cgagtgctta gcactcgcct taaaagctta ttccagaact
     2821 gttttgagat ctttcgcgaa cgcgattgcg tagacaggaa cttcgtggcc taatcctgcg
     2881 agcgtgtcca tgctgaggcg ctggctggtt ttgcccgggc caaagtcgag tatcgctgtg
     2941 atagacttat cgttcttcac cggcagcatc gatttttccc aatagagagt tttcaccatc
     3001 aggtcgtcgg tcatgcgcat gccgagttcc gcttcgttct gcaggttgtc gccgttccag
     3061 aaagagtaaa ccgggaactt caggtctgag cctttgaagt cgaagccgat ttttgcgagg
     3121 tcgggtttga atttttcgag gatcgggatc atcagctcgc agtggaaagg gcaggtcgag
     3181 cgcaggtaga cgaacttgat ctgcttcgct tcaaactccg ccttgtagcg ctcgtggaac
     3241 gctatcaggt cggcgcgcgc gccggagatg atgcggttgt tgggagtgtt gtagaggctg
     3301 acgaaaagtt ttttcgccgc gccgttaaat tctttcaccc atgcttcaat cgttgcgtgg
     3361 ctttcgccga gaacagctac catcggggct gggccctttg cgccaagcgc ttcgctcttc
     3421 gctgcttcat cggcagttgc atggatatga ggaaagactt cctgtgcacg tacacccatc
     3481 aggaacaggt actggatgta cttgtagact gcatcgtagt atgctttgcc ttcgaggccg
     3541 agcgccgtga agctcgcggc gatgaggccc tggctgtggc cgcttgcggc aacagtgtgc
     3601 ttgagcattt ctgcgcggtc gaagccctgc tggtgcaggt actcgtagtg cgcaaactgc
     3661 gtggcctgaa tcaggccgag tgagataccc gcgatcgaca tcgcgtcgtc agatggagcg
     3721 tggtcgggct cggtgagcca tttgcggaag tcgacttttt ccgggtgcgc agcggtacct
     3781 tcaaccattt tggctgcagc ttcgatggcc gaaacagcga catcgaaata gggtttcatc
     3841 gaagcttctg cgtagagctt ctgcatctct ttgaggtacg gagcgccctg gcctccgaac
     3901 tggagaaaga attttgcctg gcccgctttt gcggaatcga gtagcttgga catttgcagc
     3961 gcttacggaa atttgaacag ggcgtcgagc caaatgggtg gtttggctcc gggcttgtcc
     4021 agaagtcgtg agagactgcc ccgaaaagtt agcgagtctt cggtgcggga gctttcttag
     4081 cagccggttt tttttccgcg ggttctctct tcgccgtttc ttctgtggct tcttcgtcgg
     4141 gctttgcatc ggccgccttt tctgctgcct ggtcgcggct gctgaaaaca tagccgacgc
     4201 tcagctgcag gtagctcgaa ttgctgccgg tttgcgttga tttgcccgac tgcgccatcg
     4261 cgccgatact gatctcattg atgaagatat caaaaccgag tgaggtagtc gtactcgccg
     4321 gcacctcgaa acttgccgag taggcgttcg tcgtcttggt gccgggatca tcggtaatgg
     4381 tcgcactacc actcatgctc gagatcacaa aaaacgggct catgcggatc atactaccca
     4441 gtggaatatc tatctgcatg ccggcctgaa tgcccccgag cataattgag gtggtgagcg
     4501 tgtcggtgaa gccggtgtaa gtggtgcctg tcgggacgta atacttattg tagagcgtct
     4561 tcaatgtcag gctagtcacg ttcagcgaag gtccggcaaa gatgatggca ttaaaaagag
     4621 gcgatcgtac tacctgcagc tcgaggtttg ccgaaaaggc aaggctcaga atgctcgcgt
     4681 tgccgttttt tgccggtatc ggtgtgtagt aaccgagaaa gctcccattg gccgcataag
     4741 ctggcagcgg tgaaattggc ccaacgcccg gcagctgccc cgaaaggtac atcaggcccg
     4801 ccattccgtc gatcgcgata ctgtcagtga aggccttgcg cgccttaccg aaggcgctca
     4861 taccgttaaa ggagtactgg tcaaaatcca gtcgcaggta gctgaagtcg attttgccgt
     4921 ccgttgcgcc tggttcaaag tacgggtatg gtatcggggc cggtgccagc tggaaggatg
     4981 attctgaact atttgaggca acaagcccgc tggctgcgag caggaatacc aggacgagtt
     5041 ttccatatag gtgcatgggt acatcgagaa aaagaagaat agagtatcta ctattctttt
     5101 attgggcgtc agtgattcct ccgggttttg cgacaatggg cgtccatatg ccgctgtgca
     5161 gttttcgtgc atgacaagtg atgtaagttt acaggtctgc aggtcgacct gcacccatgc
     5221 tacgccagat cgcttcatcc cgctttttcc agaattttat caccgtcgtt attgtgctcg
     5281 cgagcatgct cgtgggtatc gagacatacc ccgaaattga gaagcagtac cacaagttac
     5341 tgcacacgct cgatttgctg attatcggca ttttcaccct tgaggtggtc atcaagatcg
     5401 gtgccgaagg ccgtcaccca ctgcgctatt tccgcgaccc gtggaatgtt ttcgactttg
     5461 tcatcgtcac tgcctgttac ctgccgtttg aaaaccagta catcacggta ttgcggctca
     5521 tccggttgct gcgggtactg cgcctgttgc gcgcgtttcc caagttgcag atactggtcg
     5581 gcgcaatctt taagagtatt ccctcgatgg ggtacgtttc gctgctcatg gggcttctgt
     5641 tctatgtcta cgcgatagca ggtgtgctgc tcttcggcaa gaatgacccg gtccactttg
     5701 gtaacctcaa cagctcgttg ctctcgctct tccagacagt gactctcgaa ggctgggtgg
     5761 aactcatgaa tatacagctg catggttgcg ataagatagg ctacgaggcg ttccgcgagt
     5821 tgtgcaccca gcccgcgcgc agttatgttg cacccctgtt ctttatttct ttcatactgg
     5881 tcggcacctt tgtcctgctg aacctgttta tcggcgtcat tctcaccggc atggaagaag
     5941 cgcgcaacga actggccgat ctcaagatga aagagtcttc aggttcgccg cgttcgtatg
     6001 acctgcagaa gatacaggca gaaattgcaa aactgaatga cgcgctgaag accctgaaag
     6061 taatacgtgt caaataccgg gacaaaaccg gggaggatgc cgatggctaa aacgtggttc
     6121 aacatacaga gtaacctgac ctttacgggc aaaggggccc tgctcgaggt cgagaacatc
     6181 atcggcaacg tcacttttgc ccagggcatc tcgaaagcga ttggcctgtt ccagaatatc
     6241 gaccgcgagt acaaaaaggc ggtcggcaac gacggcaaga ttgtcggcaa agaacgcacc
     6301 gacatactga tacaggtcga tcgtttcctg aacggcctga ttctcgtctg gaaaaagctc
     6361 gagatgcgaa acgataacat gatcatcatc gagaacaaga acgtgaactt cctgatgacg
     6421 atcaacgagc gcaacggcct gtgggaggcc agcggcaccg tgccgcactt cttcgtcaaa
     6481 ccagtcaaaa acttccagga aatctataac aacaagctta cgcccgagat cgtcgcactg
     6541 ctcaaacgtt atgccgaagt tgtcgaagac ggcatcatcg acgacgacga gcgcgaagac
     6601 ctcaaagccg gtatgcagcg ggtgttgtat tatacgttct ttctccgcac acaagttgag
     6661 aagttactga tccacgattg atcgggcttt ccagaaagtt tccgatcgaa gtctgccggg
     6721 atgtccgcca gaattgagcc gtgctctccg tgtcgccgtg gtagttaatt atcactgttt
     6781 tcgttgaaaa agcagaggat tcaaccaatc agttgtttgg caaaccacgg cggcacggcg
     6841 tacacggcgt tagccaaaaa gtatcatcgc attttctggg cgcctccagg atcatattat
     6901 actatataca gtatcgtata atgaaaacag ctgctgcccc caacaagttt gccgagtttg
     6961 tctcttcgag cgatacaccc gtactcgtcg acttctatgc cgattggtgc ggaccctgcc
     7021 acatgatggc gccgatcctg aacgatttga aatccacctg gggtgaccgc attcgcatca
     7081 tcaaggtcga taccgagctg cagccacaga ttgccgccca gtaccagatt tcaggcattc
     7141 cgacattgat actctttaaa gaaggaaagc cggtgcaccg tacctcgggc gtgatgccct
     7201 tgcagcggct cgtgcaggaa tacggcaagt acctatagct agctaccgcg tcaggtagat
     7261 gtcgaaccgc cggttcttgc cggcgaaaat atgtttcgct gagtagagtg gagccgtatc
     7321 gccgaaaccc tggtacgtga tgcggtcagg ggtgacacct ttctcgacaa aaaaatcgcg
     7381 cagcttgcgc gcagccgatt cgctcaggct gaggttctgc tcaatctttc cctttaaagc
     7441 cgtgtgcgct tcaatcttta cccggtattt cgggtagcgg tcgagcagcc ggtaaacggt
     7501 ctgcatgcgg tcagtcacag gatcttccag gtttgcgtaa ggcagccgga tgagaaaacc
     7561 agtagtggtg gcgactgcga gcgcgcctga ttcaaaacgc cgcggcgcgg ttgtcgtact
     7621 gttacccagt gtatcggttg ccgtcagcgt gtattcgtac tgcgtcagcg attcgatcag
     7681 cgcaccactt tctgtcttac cgtcccagta gatagtctgt gggatctccc cctgtcctga
     7741 aaaacggcgc acgggaatca gcctgacgcc gtttgcctcg ggggcaacct cgctgatcgt
     7801 cagctggtac gaatcgatag gcgattggtc gtgccagaca atacggaaag cctgctcttc
     7861 gttatcgccg tcgccgtcgg gggcaaactg ccgcatcgtg aggtcggcgg taatttctgc
     7921 ctcagggccg gtgctatcga ggctgagctt cagcgccggc gattcgggcc cgttgccgtt
     7981 ctcatagcgc gcacggaata aagcacagta gttgccgtcg ggcgccggtt taccgccggg
     8041 gctttcgccg gcccaggtat agaccggttg tttgtccttg cgcggctgct tcttcgcgtc
     8101 atcgacttta accgttgtta cagcgatgcg gtgcacgaaa tcttctttct tcgggccaca
     8161 ctgccggctg atcagaattt ctccctcttt ataaccatca ccgaatgcga cagaagggtt
     8221 gaagctgatc gaggcgagtt tcgggttctt ggggttgagc gagagcgccg aactgcgcag
     8281 aaacacctcg tcgaccctgc gcgagatata gaaattgccc gattcggcat ggacgctgtt
     8341 gccggctgcg tcgtggccca gcaggcggta gcggtagacg ccttcttcgg ccagctcgtc
     8401 gttatccatg cgaccatcaa aatcaaagcg ggtgggagca ttttcgttcc acttccacga
     8461 gcggattttg atgccctgcg cgttgacaat cgtaccttcg taacggtcgg ccggggtggt
     8521 atcctgcgtg atcacgagca ggtcacgcct gccatcgcca ttgggtgaga tcaggtcgtc
     8581 gccgagttca acgctcgccg cgggtggctt cgtgtcgata tgaacctggc ccgagacggg
     8641 tgcgctttcg ttgtttttct cgtcgatcgc cctgaagtcc caggtgtagg caccgtctgg
     8701 cagcgcgcgg tcgggcgtct cttcttcaaa cactgcatcc ttgaccggct tcttctgctc
     8761 tgtctgtacc cggccgttcc aggtgaggcg ttcgggtatg tccgagcggg cccggggagc
     8821 gacaaagctg cggacgaaca gcgaccagct gaaatctttt tcccttatac gttcgtcgga
     8881 gcgctggcgg tagacaaggt tacccttgct gtcgagcacg cggaattcgt agaagacgat
     8941 cgggcttttg tcgcgcaccc gtgggttgaa caccacgaga tcctgcaccc cgtcgttgtt
     9001 cggtgagaaa taatcgcctt ccgtttcgac gctcacctcg ggagcatcgc ggtcgacgcc
     9061 gccgagccgt acgcccaggg tcaggctgtg caggtcgctg cgttcgctgt tggcaagtcc
     9121 cactgcgtaa aagaggtaag tgtcgccaaa atcggccggt agcacggcgc caaggcccag
     9181 gtgaaaaccg ttgccgacct gccgaaagtt cgaggcgccg taaccgaatg acagcatggc
     9241 gaagcggtac tgcgcctgca ccccgaccag caggggcagt ttgtcaaagc gctctgtacc
     9301 ataggttgag gtgatgagcg caaggttgaa gctcgtgctc tggtacatac cggtgagaaa
     9361 tcccacatga gccgaaggtg aagcaagcag cgaggtttcg ccgacaggaa tcgccaggtt
     9421 ttgcgtgcgg aaaaagaccg agggtgagta gagcccccag ccgtcgttac cggcaaaact
     9481 tgtgtgccac ttgctgtcga agagaagcgc gggatcaagc ccaaggccaa aggcattgct
     9541 cgcatcgccc gtagtataac gcggcgtgat gctgagacct gtcgcgacgc gccgagaaag
     9601 aaaactaccg tagccgaagc tgaggctcgc cgtcttgtca cgggcctgca ggtactcggc
     9661 agagagcatc aggccgccaa aggggctgaa gagggcgccg tcggcgtaaa agcccgtgag
     9721 accctgacca aaaatgcccc cggcgaagaa cgtgttctgt tcgccataac ccaggagggc
     9781 agggtttacc gctgctcctg cctgaacggg catcgactgc gtcatttgcc cggcaagcgc
     9841 ctggtggaat ggccgcagcg acaggtggcc atagtcggca gtgggccggt cagccaaggt
     9901 cgggcaaaac ggcgaggcca acaacagcag caggcaagcg gcaagcaggc gaaacttcgc
     9961 gggccagcgt tcgggatggc ggtcagggcg gcgcaacgcg gtatttttcg cgatgaaaga
    10021 cagcgcggca aacgcagaat aaatgctaca agtttgtgtg aattctctcg aaaatactat
    10081 atgcaggaca tagatttcag tcgctattac cgtaaaggcc ggcggacgta tgcggcttcc
    10141 gaagcgcctg aagtgcgcaa gacgggtgct cccgttaata agctctctct ctacctcgcc
    10201 attgcttttt catcgctcgc gatcggcttc atcggcggca cccagctgca gaagaacaag
    10261 atgatgcgcg agctcgaaaa ccgggtggca cccgaaagcg cgcagaatgg cacgatggtg
    10321 acggccaacg tcgcggctgg ccctgcacca gaggcagaaa agccgcccga gactattcgc
    10381 accacccgtc ccgatgaagg cgcacgcgca gtccgcccgg acaaaggcct tgaaggcagt
    10441 ttcctgatcc tggcgaagat ttaccccgat gaaaaagagg cgtcgcttgc aggactaaac
    10501 ctcaagcgca atggcatgaa ggctttcatg gctcgcaatg gcaagaaagt gaagctctat
    10561 gtcggcccca tcgagggaaa aaacagtgca tacgaagcgc tcgcgcaact gaagaagaat
    10621 cccgagttta ccggggcaat tctctaccgc aaatagtctt tccggcaggg cgcacaagca
    10681 gtcagcgccc gtcagccatg cccagtgcaa taaatccgca gatccgagac gccaggccgg
    10741 gcgatgaaga cgccatagcg gcgctgctcg agccttatgt gctgaagaag attgttctgc
    10801 gtcgttcgcc tgcagaaatc agggagaacc tcggccagac aatcgtctac gaagagaatg
    10861 gtcgcctgct ggggaccacg agcctcgtct tctttaccga tacgctctgc gaaattcgtg
    10921 cacttgtcat tgccgacgaa ctacagggca aaggtgtcgg ccggcagctc gtgttagccg
    10981 ccgaagaaaa ggcgctgcag cattcggcac aacgtccatt gcgcttcttt gctctcacct
    11041 atacaccgga gttttttgag cgttgcggtt acgagcgcac gacgaaagat aagttcccgg
    11101 aaaaaattta cgaggtttgc aacttctgcc tgcgcaaaga tgactgccac gagatcgcgg
    11161 tgcagaagac gacgaactaa aaacgtcgcg ccatagatac cgcgccaaaa aatttccgca
    11221 agcagttcaa aaaaattgtg atcggcattt tcatcaagga aaaatttttc aaggaagtag
    11281 ttgacggcgt acgagtgctg gatacgatgt gacataattt cgctgatgag acataatacg
    11341 actggctcga cgaacggaag gcaactcgcc taagaatacg atggaactct ttcgtaaagt
    11401 tcgtccattg ctcgtcatcg gcgctgccgt aacgctcgca atctttgcga cgatttacct
    11461 cttccgtttc tttaatcgtg ccaacatcga gaaggcgctc gctggcaaag acggtttctt
    11521 tgtctccttc gtcgtgacct cggctaacga cgacaagaaa gtgcaggttg tttccgtggc
    11581 gcagatcttc ccggcgacac agcgcatcgg agttatctct ttctacccgg ctatccgcct
    11641 gaaggacaat gaacccagcc tcgaggagat gtacaaatcg ggcggggtgg cagcaatcaa
    11701 cagtgccgtc tcagaatttc tgggtgagcg ggtcggtatg catgtcaagc tgcgtgatac
    11761 cgcgctggcc cactttgtcg atgtcctggg aggaatcgac tatttcctgc cctccggcga
    11821 cctgctcaaa gacgaaaacc tacccaaggg ccagtttatc ctcgatggca gcctggttcc
    11881 gcgcctgctg aacccgcctg aaaagaacga atataccccc gcttttaaac tcttccgcta
    11941 ttattccctg atgctcaacg cctggaacca gcgcagccgg ctttggcccg taattaagga
    12001 tgaaaagtcg ttcgcgctgg ctgtgcgcaa tgttgagacc aacgcctcgg cgcatgatct
    12061 gttcgtgctc gcccagacct ttgcaacgca gaagtcgtgg gtgccgattc tgctcgaggt
    12121 gcccgtgcgc cgcgtgaaag atgccttcta cgtcgacaaa gactcggccg cgctctactt
    12181 tcgtaacctg accaagcagc tcacacagaa agagcacccg tttatcaacg aacctcccaa
    12241 aatggaggta aagaacggaa cgcacgtgcc caacctggcg cgaaccatgc gggctgaact
    12301 gtcgcgcaag ggcgtgcaga tactcgaatt caccaacgct gaccacaa
//
LOCUS       DAOQJF010000050        12266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2575_length_12266_cov_6.075178, whole genome shotgun sequence.
ACCESSION   DAOQJF010000050 DAOQJF010000000
VERSION     DAOQJF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12266
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2575_length_12266_cov_6.075178"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLF85_04260"
     CDS             complement(<1..308)
                     /locus_tag="PLF85_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18902.1"
                     /translation="MTKTTALKRFLRALALLLGVCLFLVTTADAARRKKGRVKPRKAA
                     AVSARAATAEESESESLPVEAAGATEGSGNSAVVVSSSNDEIVPGSGSGVVAEGEAPG
                     A"
     gene            complement(310..876)
                     /locus_tag="PLF85_04265"
     CDS             complement(310..876)
                     /locus_tag="PLF85_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18903.1"
                     /translation="MNWPNIRKFPVSAKTLIAIYTLLMGSILVVALALSSWHHGLVTE
                     KEAKKSAPAETSAPAAAKAEVKPDDGQSSGVVIEADTESTVKVKTLDWLVYENFKLGH
                     THLNGQSLVYFSLMVLFLMSTASERSKIAWGVLLAFSVILHTFGLFFMHKEPFGMMLR
                     VSGALLALSITVMCFYIFVDTIKKPATS"
     gene            complement(963..2381)
                     /locus_tag="PLF85_04270"
     CDS             complement(963..2381)
                     /locus_tag="PLF85_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE18904.1"
                     /translation="MFAKAPERRMVSVRMILFIAWIVLIVSLFWDPVTPVLTQPDSGT
                     PFALSNKQVMVQGQILEQKPYAMGARMFWTMILPCVPLFLMVFGHEAWRRICPLSFAS
                     RIPYYLGLQWLRKTFNRQTGKVERRPAVIAQDSFLARWHFPLQFIFLSAGVTARLLFI
                     NSDRAVFAFFFIGIITAAIITGFLYGGKTWCNYLCPIAPMQRIYTMPRGILETPAHAP
                     KQAVSQSMCRVPGKDSDQSVCVSCIQACPDVDLERSYWEIFMRPGSRFAYYGYYGMVL
                     MFYVYYYLYSGGWDYYFSGAWTHEENAQLNQLMNPGFFINGQSYNIPKIIAAPLAMTA
                     AILLAYAFWFGVELLYARIARRVKPGISQETIRHHMLMVCAWATFNTFYLFAGRPNIA
                     LMPLWLQTTVNTTIVTASTIWLMMNILRRAEDHAEESLAAALRSRVKQSYDSVKDFVR
                     GKPVENLDAREVNILAKLAKGD"
     gene            complement(2381..4129)
                     /locus_tag="PLF85_04275"
     CDS             complement(2381..4129)
                     /locus_tag="PLF85_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid-cluster NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE18905.1"
                     /translation="MTVAAVQDKLPQAVFALFVWQCDLLSEVTAAEAEAFFRFLDSAG
                     TYGLSKEACEELQRSYSKQWRDYLRSGAMRPEQLNQFVNQVDGSARQALLGLLQALTD
                     AAPRKKRQLQSSLSNLLNRLVNGHDAALTGLQAIYRESRFYGDEVADAAPAAPAEAVV
                     ATETTVKVVEETHVVSAGTTAVAETTTQATVSSNQRVMAVHKMVPWTEHDAVSPERLQ
                     FWTKGTRLMRCIGIVDRSHDFRSFQFVSEEPTLFFYKPGQFVTLELTIDGKRYLRSYT
                     ISSSPSRPHLIEISVKRVKGGLVSNYLHDHLHIGDSVLMKPPGGKFHCFDTTSDKYLF
                     LAAGSGVTPMLSMARWWHDIGAQPDVIFFHSIREPEDAVFTEDMMMFTSANRKFKYLA
                     SFTSPQLPEHWPGMKGRFSEDKLLQIAPDFKERMVFTCGPDSFMKNVKLILEKNGFEI
                     KERFRQESFGAPREKGSAEPTPITVFETKAVTQPKTAPKKPAETGDAVVHFSLSAMEI
                     FGGDMDVPILDLAEEVGVQIPSSCRSGTCGTCRALKIRGEVECDDSPALTDVDREAGY
                     ILTCVSRAKNYVELEV"
     gene            4243..5187
                     /locus_tag="PLF85_04280"
     CDS             4243..5187
                     /locus_tag="PLF85_04280"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HNE18906.1"
                     /translation="MEQNSHLRISFSARGGELFVLLIKNLFLTLITLGVYSFWARVNV
                     QRYFYEHTNLAGGRFGWHATGKERFIGFLKASVIMAAIIGVNALLVKLSPYLGMILPI
                     AVIILLPAIIVAQYRYRLARTSFNQIRFRFTGRAGNLAAIVFKGVLLTIVTFGFYGPW
                     FAVDMRRYLYENTRIGSSNLQYEGKGGEILSMYIVAILLTIVSFGIYRFWFTAKIANY
                     HTSRTRFQGAPLKGDVDGGDVFIANLVGQMLTFVTLGIYLPWYIVRLQKVMLEGISLT
                     AEPDYSQMQAQVDTGASALAEGLADAASLLDNIADFLS"
     gene            5184..6389
                     /locus_tag="PLF85_04285"
     CDS             5184..6389
                     /locus_tag="PLF85_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNE18907.1"
                     /translation="MIRQAPAQYLDGIDPRPRTGRLVFDEAHGELHFYETDTSGIERF
                     IFSVNKNHTFEIRSRGGEHVIEWREAGNASSRMLTLGDIDFVRLVRDRFLAHKNIFWR
                     YATLVWSESLGKVALALIAALAVTGIAGWFFMQNSYKVIPIAWDKKIGEQAEPGLKEF
                     GLTCASPQTVRDLKKLIPYLAQPGTPYSYDIQIIKSNVENAFALPGGKLTFFSETIKN
                     AKSYGELAGILAHEIGHVERRHGMQQLSQYMTIRVILALAFGMTDDATLIATAADAGA
                     LLLLLKNSRDHERDADAYAAEKLVAAGISNRPIREFFERIAREHKKDMQNVPDFILTH
                     PADNERIRFFEKYEIRHKAAFRTAAAKLNPEISALLKRKPVLAAECIAPLKSTKKNAD
                     DDEEGDAEP"
     gene            6570..7487
                     /locus_tag="PLF85_04290"
     CDS             6570..7487
                     /locus_tag="PLF85_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE18908.1"
                     /translation="MAVSSSDDLRTGAIKQVVGVGTRLAFNRKIPKPMIQLGLNALGV
                     AIGAAPGCSLMEGSIDTIAYDFWTPEQLKPGRLLIYSHGGGYTMFSHKTHRTLASRLA
                     VEFEAQAIVYDYRLSPEHRFPAAVDDALAVYQHVLRQGYDPRNIILAGDSAGGGLTFA
                     LLLAARDHNLPMPGLAIGLSPWVDMTLSGYSFQENPHKDVMLSPDGVRHFREMYLDGA
                     DPKHPYASPLFGDLRGFPPVMLQAAGDEILRDDSVLFAAALRQAGIRVELDVSPGLFH
                     VFEFAWRILPEADEAIIRMGDFVRRHYAD"
     gene            7572..8675
                     /locus_tag="PLF85_04295"
     CDS             7572..8675
                     /locus_tag="PLF85_04295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE18909.1"
                     /translation="MQEHSALNQPDTFIEHYERKIHSQKQLIELSKALNSNLELRSLI
                     DAILNVSIAQSRCLQAGIFLVPEIDHHSLVLDENVVGFEVDNAATFVIPYKSDLVKLL
                     TEYPTLLTLSEIRNIAAATDHSSLAQVSFRDAVTQLSKLNNELLLVPLKAKGRTNGIL
                     VMGPKSDGEPYLAEEKAFLSDLASLAAIAVENARLYELATVDMMTKLKIHHFFQARLR
                     EELELARDNNKPVSLLLTDIDHFKKFNDTYGHQVGDIVLKEVARVLISLARGNDVPAR
                     YGGEEFALILPGKDIDSAKAVAEKYRERIEKLTVKNPTDKGDKLLRVTVSVGVATFAP
                     ATDHDNKHLIERADQALYAAKHAGRNRVEIKIA"
     gene            8691..9527
                     /locus_tag="PLF85_04300"
     CDS             8691..9527
                     /locus_tag="PLF85_04300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE18910.1"
                     /translation="MADLKTITLKKTTAFALAISAWILIALLDSQITFEIGFSIFYLI
                     PISLATYYGGFRQGLVISVLSALAWGFNDWYTGHRYTNHWMPLWNIFMRLGYYILHSY
                     MLRRYTTLLAYTSSLAYTDTLTGAFNTRYLARHIARLRETGRTAKQPVTLAYIDLDNF
                     KEVNDTHGHDEGDQVLRVLAEETRKTIGENDIFARLGGDEFVVVIPERDFTAAKQIIE
                     QLAMNVLAAFRRRGWPVTLSIGALTYNHAAKIPVSPMQQVDQLMYKVKKTGKAAIRHQ
                     KA"
     gene            9593..10045
                     /locus_tag="PLF85_04305"
     CDS             9593..10045
                     /locus_tag="PLF85_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNE18911.1"
                     /translation="MAELSKDIVGRKMEPFTFTVERGKVKEFLNAIGETNAIYHDVNA
                     AKAAGYADTPIPLTFQTAFTFWGYGDKFWSDLDSFGIDTKRLLHMKEEYTYLEPIYPG
                     TTVSCQVEVIDVKVGKMNMVTFKNTYSDGKGKIFIEAEMAIVIRPEGM"
     gene            10057..10476
                     /locus_tag="PLF85_04310"
     CDS             10057..10476
                     /locus_tag="PLF85_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNE18912.1"
                     /translation="MNKIKFADYNEGQDIPELRVGPIKQMDLVRYAGASGDFNPIHND
                     VEFAKSAGLPGTIAHGMYIMALMGRQVTNWAPPTQVKFFGVKFKGMSLPGETMVFTGK
                     IKKKKEENGDKLLMVSLTAANDKGEVKVSGDLTVKAE"
     gene            10492..10821
                     /locus_tag="PLF85_04315"
     CDS             10492..10821
                     /locus_tag="PLF85_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE18913.1"
                     /translation="MDIAFADIGSHKVVKVMGDVDLYNVGDLKRSVFELIDEGEVESL
                     IIDMSSVNYIDSSGVGALVAAQKKMKTQGGKFGLLGLTEDVLNILKLATLDQFFKIYE
                     TEAQLPQ"
     gene            10867..11934
                     /locus_tag="PLF85_04320"
     CDS             10867..11934
                     /locus_tag="PLF85_04320"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNE18914.1"
                     /translation="MKTVLLVYGGQSGEHEVSCVSAAYLEQTIGEAGYTPVPVYVDRT
                     GVWHWQQKVHKTPADNAANPATLSRVGSNVVLRSAASSVAVQVAFPIIHGTAGEDGSL
                     QGYFEVLGLPYVGAGVASSAVCMDKALMRALFAVNGIPQVQYFVITRADKADPRQVDK
                     QISESFGYPVFIKPCNMGSSVGVHKVSNASALAAAISDAARFDDHLLCEQGLYVRELE
                     IAIAGNYPEYLTSGVGEIKVNHEFYSYEAKYLDPKGADLFIKAEISEAQAADIRKLAE
                     QAFASVRGDGFARIDFFLDKDSGKLYLNEINTLPGFTPISMFPQLFRAAGVSGADITK
                     RLIEWGEARCQRLQRLRAASR"
     gene            11939..12244
                     /locus_tag="PLF85_04325"
     CDS             11939..12244
                     /locus_tag="PLF85_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18915.1"
                     /translation="MPGDYTLSYHFFGINDDRNHFELFIDADGASDLATWQHHPRAAG
                     AKHDRRWRFRRAKAHRRVYLGFSGPVSNNRGRLRILRQGKVHDRRKTGPQDSILVTL"
BASE COUNT         2852 a         3466 c         3335 g         2613 t
ORIGIN      
        1 gcgccggggg cttcaccctc ggcgacaaca cccgaaccgc taccggggac tatttcgtcg
       61 tttgaagaag aaacaactac cgctgaattg ccagaccctt cggtggcgcc tgcggcttca
      121 accggcaacg attccgattc gctttcttca gctgtagcgg cacgcgcaga gactgctgcg
      181 gctttgcgtg gcttgacgcg ccctttcttg cggcgggctg catcagccgt ggtaacgaga
      241 aagaggcaga cgcccagcag cagtgcaagg gcgcgcaaaa atcgtttaag tgcggtagtt
      301 tttgtcattt caggaggttg cgggcttctt tatagtatcg acgaagatgt agaagcacat
      361 gaccgtgatc gacaacgcca acagcgcacc gctgacccgc agcatcatac cgaagggttc
      421 tttgtgcatg aagaacaggc caaatgtatg aagaatgaca gaaaacgcga gcagcacacc
      481 ccaggctatc ttgctgcgct ccgatgcggt tgacatcagg aagagcacca tcagcgagaa
      541 gtacacgagg ctctgcccgt tcaggtgcgt atgcccgagc ttgaagtttt cataaaccaa
      601 ccagtcgagc gtcttcacct tgacggtcga ttcagtatcg gcttcgatca ccacaccact
      661 cgattgcccg tcgtctggct ttacctcggc ctttgctgca gcaggcgcgg atgtttctgc
      721 aggcgcagac ttcttagctt ctttttcggt gacgagaccg tggtgccacg agctgagcgc
      781 cagtgcaacg acaagtatag aacccatgag caaagtgtag atagcaatca acgtctttgc
      841 ggacacggga aatttgcgga tgttcggcca gttcataacg gctaacgtaa ggtgcgttag
      901 cctcggggca agaacttttc ctgtttggcg cccccctgat ccgggggcgc gttacgtggt
      961 ggtcagtcgc ctttcgcgag cttggcgaga atattcactt cgcgcgcatc gagattttct
     1021 acaggcttgc cgcgcacgaa atctttgacc gaatcgtacg attgtttcac ccgcgagcgt
     1081 agcgcagccg ccagcgactc ttcggcatgg tcttcagcac gccggagaat gttcatcatc
     1141 agccatatcg tgctggcagt aacaatcgtg gtattcactg ttgtctgcaa ccagagcggc
     1201 atcaaagcaa tgttcggccg gcctgcaaag aggtagaacg tgttgaatgt cgcccaggcg
     1261 caaaccatca gcatgtggtg gcggattgtc tcttgcgaga tgcccggttt taccctgcgg
     1321 gctatgcgcg cgtagagcag ttcaacgcca aaccagaacg cgtaggcgag cagaatcgcc
     1381 gcagtcatcg cgagcggtgc cgcaatgatc ttgggaatgt tgtaagactg cccgttgatg
     1441 aagaaacccg gattcatcag ctggttcagc tgcgcatttt cttcgtgcgt ccaggcgccc
     1501 gagaagtagt aatcccaacc gccgctgtag aggtagtaat agacgtaaaa catcagcacc
     1561 ataccatagt agccatagta ggcaaagcgg ctgccagggc gcatgaagat ttcccagtag
     1621 ctgcgctcga gatcgacgtc gggacacgcc tgtatacacg agacgcagac gctctggtca
     1681 ctgtctttgc ccggtacgcg gcacatcgac tggctgaccg cctgcttcgg tgcatgcgca
     1741 ggcgtttcga gaatgccgcg cggcatggtg tagatacgct gcatgggagc aatcgggcag
     1801 aggtagttgc accaggtttt gccgccgtac agaaaacccg tgatgatcgc cgcggtgatg
     1861 ataccgataa agaagaacgc gaacactgcc cggtctgagt tgatgaagag caggcgcgcc
     1921 gtgacaccgg cgctcagaaa aataaactgc agaggaaaat gccagcgcgc gagaaatgaa
     1981 tcctgcgcaa tgacggctgg ccggcgctcg accttgcccg tctggcggtt gaacgtcttg
     2041 cgcagccact gcaggcccag gtagtacggt atgcgcgaag caaaagacag cgggcagatg
     2101 cgccgccacg cctcgtgccc gaacaccatg agaaagagcg gcacgcatgg cagtatcatc
     2161 gtccagaaca tacgagctcc cattgcatag ggtttctgct cgagaatctg tccctgaacc
     2221 atcacctgct tgttgctgag cgcgaatggc gtaccgctgt cgggctgcgt caaaacggga
     2281 gttacgggat cccaaaaaag ggaaacgatc aggacaatcc atgcgatgaa gagaatcatt
     2341 cgcacagaaa ccatgcggcg ttcgggtgcc ttggcaaaca tcagacctcc agttcgacgt
     2401 aatttttcgc gcgggaaacg caggtaagaa tgtagcctgc ttctcggtcg acatcagtta
     2461 gcgccgggga atcgtcgcac tcaacttcac ccctgatctt gagcgcgcgg caggtgccgc
     2521 aggtccccga ccggcaggac gagggtatct ggacgccgac ttcttcggcc aggtcgagaa
     2581 tcggaacatc catgtcgccg ccaaaaattt ccatggcaga gagggaaaaa tgcacaacgg
     2641 catcgccggt ttcggcaggt ttcttcggag cagtcttcgg ctgcgtgacg gccttagtct
     2701 cgaagacggt aatcggtgtt ggttcggcgc tgcccttctc acggggtgcg ccgaaacttt
     2761 cctggcggaa acgttcttta atctcgaagc cattcttttc cagaatgagc ttcacgtttt
     2821 tcatgaacga atcgggaccg caggtgaaga ccatgcgctc cttgaaatca ggcgcaatct
     2881 gcagcagctt gtcttcagag aaccggccct tcatgccggg ccagtgctct ggcaattgcg
     2941 gggaagtaaa agaagccagg tacttgaact tgcgattcgc cgacgtaaac atcatcatgt
     3001 cttcagtaaa gacggcatct tcgggttcgc gtatagaatg gaaaaaaatg acgtctggct
     3061 gcgcgccgat atcatgccac cagcgcgcca tcgacagcat cggggtaact ccgctacccg
     3121 cagcaagaaa caggtacttg tcgctggtgg tgtcaaagca gtggaacttg ccccctggtg
     3181 gcttcatgag cacactgtcg ccgatgtgca ggtggtcgtg caggtagttc gagaccagcc
     3241 cacccttcac gcgtttcacc gaaatttcaa tcaggtgcgg ccgcgaaggg ctggaagaga
     3301 tagtgtatga acgcaggtag cgtttaccgt cgatggtgag ctcgagtgtt acaaactggc
     3361 cgggcttgta gaagaaaagt gtcggctctt cagacacaaa ctgaaaactg cggaagtcgt
     3421 ggctgcggtc gacgatgccg atgcagcgca tcagccgcgt gcccttggtc cagaattgca
     3481 gacgctcggg actgaccgca tcgtgctcgg tccatgggac catcttgtgc acagccatca
     3541 cgcgctggtt cgagctgact gtcgcctggg tcgtggtttc tgcaacagcg gttgttcccg
     3601 ctgacacaac gtgagtctct tcaaccacct ttacggtggt ctcggtcgcc actactgcct
     3661 cagcaggtgc ggccggcgcg gcatctgcaa cctcatcgcc atagaagcgg ctttcgcgat
     3721 agatagcctg tagaccggtc agggcagcat catgaccgtt caccagcctg ttcagcaaat
     3781 tgctgaggga gctctggagt tgacgttttt tgcgcggcgc ggcatcggtg agagcctgca
     3841 gcaaacccag caaagcctgc cgcgcgctgc cgtcgacctg gttaacaaac tggttcagct
     3901 gctcgggacg catggcaccg ctgcgcaggt aatcgcgcca ctgcttcgaa taagatctct
     3961 ggagctcttc gcaagcttct ttgcttagtc cataggtacc agcagaatcg agaaaccgga
     4021 agaatgcttc cgcttctgcg gccgtcacct ctgaaagcag gtcgcactgc cagacaaaaa
     4081 gagcgaatac cgcctgcggt aatttatcct ggactgcggc aactgtcatc gtgtgctgtg
     4141 tgcagctgcg tagaacggac agcttgtcaa tggctaatga aacgtgacct gtcaaaaatg
     4201 tgattgctgc gaccaattta gctgaacatt cgtccgcttc atatggaaca gaacagccac
     4261 ctgcgtatat cattctctgc ccgcggcgga gagctctttg tcctgctcat aaagaacctg
     4321 tttctgacgc tgatcacgct cggcgtctac agcttctggg cccgcgtgaa tgtgcagcgc
     4381 tacttctacg agcataccaa cctggccggc ggccggtttg gctggcatgc cacgggaaaa
     4441 gaacgcttta tcgggtttct caaggcgtcg gtcattatgg cagcaattat tggagtgaac
     4501 gcgctgctgg tgaaactttc gccttacctc ggtatgattc tgccgattgc ggtgatcatt
     4561 cttctgcccg ccatcatcgt cgcgcagtac cgctatcgtc tcgcgcgcac ttcttttaac
     4621 cagattcgct ttcgtttcac cgggcgcgca ggcaacctgg ccgcgattgt gttcaagggt
     4681 gtactgctga ctatcgtcac ctttggcttc tatggaccat ggttcgccgt agacatgcgc
     4741 cgttacctgt acgaaaatac acgcatcgga tcatccaacc tgcagtacga aggcaagggc
     4801 ggcgaaattc tctcgatgta cattgtggcg attctgctga ccatcgtctc tttcggtatt
     4861 tatcgtttct ggtttacggc aaaaattgcc aactaccata ccagccgcac gcgcttccag
     4921 ggagctcccc tcaaaggtga tgtcgacggc ggcgatgtgt tcatcgcgaa cctggtgggg
     4981 cagatgctga cctttgtcac gctcggtatt tacctgccgt ggtacattgt ccgcctgcag
     5041 aaagtcatgc tcgagggtat cagcctgact gccgaacccg attactcgca gatgcaggct
     5101 caggtcgata ccggcgccag cgcgttggca gaaggcctgg ctgatgccgc atcgctactc
     5161 gacaacatcg cggattttct ttcgtgatac gacaggcccc tgcgcagtat ctcgacggca
     5221 tcgacccgcg gccacgcacg ggtcggctgg tctttgacga agcgcacggt gaactgcatt
     5281 tttacgagac cgatacttcg ggcatcgaac gcttcatttt ttccgtaaat aagaaccaca
     5341 cgttcgagat acgctcgcgc ggcggcgagc acgtgatcga atggcgtgag gcaggtaacg
     5401 cctcaagccg catgctcacg ctcggcgaca ttgatttcgt gcgactggtg cgggacaggt
     5461 ttctcgcgca taaaaatatc ttctggcgct atgcgaccct ggtatggtca gaatcgctcg
     5521 gtaaagtcgc gctcgcactc atcgcggcgc tcgccgtgac tggcatcgcc ggctggttct
     5581 tcatgcagaa cagctacaag gtgataccca ttgcctggga taaaaagatc ggtgaacagg
     5641 cagagccggg gctaaaggaa ttcggcctga cctgcgcttc gccgcagacg gtgcgcgacc
     5701 tcaagaagct gataccctac cttgcacagc cgggcacacc ctactcttat gatatacaga
     5761 tcatcaagtc gaatgtcgaa aacgccttcg cgctaccagg cgggaaactc accttctttt
     5821 cagaaaccat aaaaaatgcg aaaagttatg gcgaactcgc aggtatcctg gcgcacgaaa
     5881 ttggacacgt cgaacgccgg cacggcatgc agcagctgag ccagtacatg acgatacgcg
     5941 tgatactggc gcttgccttc ggcatgaccg acgatgcgac gctgatcgcc acggccgccg
     6001 atgcgggagc tctcttgtta ctgctgaaga attcgcgcga ccacgaacgc gacgccgatg
     6061 cctacgccgc cgagaaactc gtggcagccg gcatctctaa ccggcccatc cgcgagttct
     6121 ttgagcgcat cgcgcgtgaa cacaagaaag acatgcagaa tgtacctgac ttcattctga
     6181 cgcaccctgc tgataatgag cgtatccgct tcttcgagaa atacgaaata cgccacaagg
     6241 cggccttcag aacagcagcg gcaaagctca atccggaaat cagcgccttg ctcaaacgca
     6301 agcctgtgct cgcggctgag tgcatcgctc cgctgaaatc cacaaaaaag aatgcagacg
     6361 atgatgaaga gggtgacgca gaaccctgac agcgaaaaaa aagcccccgg ggttagccgg
     6421 gggcaaattc tcagtgagac aacatgaagg tgccaaggca ccaatctgcc tgtacgcaag
     6481 caagaaccat gccaaaatat cactcccgct agtttcgtaa gggtggttat cggctcgacg
     6541 ctcgcaaaag cattcgtgaa ccggagcgca tggctgtttc atcgtcggac gatctgcgca
     6601 cgggtgctat caagcaggtt gtcggggtcg gcacgcgcct cgcgtttaac cgcaaaatac
     6661 cgaaacccat gatacagctg ggtcttaatg cgctcggcgt ggctattggc gctgcaccgg
     6721 gctgctcgct catggaaggt agcatcgata ccatcgcgta tgatttctgg acgcccgaac
     6781 aactgaaacc cggccgcctg ctcatttaca gccatggtgg cggctacacg atgttctcgc
     6841 acaaaacgca ccgcactctc gcctcaaggc tcgcggtcga gtttgaagcg caggcaattg
     6901 tctacgatta ccggctctct cccgaacatc ggtttcccgc ggctgtcgac gacgcgcttg
     6961 cagtctacca gcacgtgctt cgccagggtt atgacccacg caatatcatt ctcgccggcg
     7021 attcagcagg cggcggatta acatttgcgc tgttacttgc tgcgcgagac cataacctgc
     7081 cgatgcccgg cctcgcgatc ggcctgtcac cgtgggttga catgacactc agtggctaca
     7141 gcttccagga gaatccacat aaagacgtga tgctctcgcc cgacggcgtg cggcatttcc
     7201 gcgagatgta cctcgacggc gctgatccaa aacatcctta tgcatccccg ctgttcggcg
     7261 acctcagggg atttccccct gttatgctgc aggcagcggg cgatgaaatc ctgcgcgatg
     7321 attctgtgct ctttgctgca gcactcaggc aggcaggaat tcgagtggag ctcgacgtct
     7381 cgccgggtct ctttcacgtt ttcgaatttg cctggcgcat tctaccagaa gcggacgaag
     7441 ctatcatccg catgggtgat tttgtgcgcc gccattacgc tgattaacat ttggcggcgg
     7501 tgccgccaaa tgttgttccc ctaagacttt ttttgcttgt accggccaat cggctttgcg
     7561 ttcggtatcg cgtgcaggaa cactctgcgt taaaccagcc cgacacgttc atcgagcact
     7621 acgagcgcaa gatccacagc caaaagcagc tgattgaact ttcgaaagcg ctcaactcga
     7681 atctcgaact gcgctcgctg attgacgcca ttctgaacgt gagtattgca caaagccgct
     7741 gcctgcaagc tggcattttt ctcgtgcccg agattgacca ccactccctt gtgctcgacg
     7801 aaaacgtcgt cggcttcgaa gtcgataacg ctgccacatt tgtgatacct tacaaaagcg
     7861 atctcgtcaa gctgctgaca gaatacccga cactgctgac cctcagcgaa atccgcaaca
     7921 tcgcggcggc aacagaccac tcgagcctgg cgcaggtcag ctttcgcgac gcggtgacgc
     7981 aactgtctaa actcaacaat gaacttctgc tcgtgccact gaaggcaaaa ggccgcacaa
     8041 acggaattct ggttatggga ccaaaatcag acggcgaacc ctatctcgcc gaagaaaaag
     8101 cctttctctc tgaccttgct tcacttgctg ccatcgccgt cgaaaacgcc cgcctctacg
     8161 aactggcaac agtcgacatg atgaccaagc tgaagatcca tcacttcttc caggcacggc
     8221 tgcgcgaaga gcttgagttg gcgcgcgaca acaacaaacc ggtgagcctg ctgctgaccg
     8281 atattgacca tttcaagaag ttcaacgaca cgtacggcca ccaggtcggc gacattgtcc
     8341 tgaaagaagt agcgcgcgtc ctcatcagcc tcgcacgagg caacgacgta ccagcgcgct
     8401 acggcggtga agagtttgcg ctcattttac ctggcaaaga catcgactcg gcaaaagccg
     8461 tcgcagaaaa ataccgcgaa cgtattgaga agcttaccgt aaaaaatccc acagacaagg
     8521 gcgacaaact tctgcgggtt accgtttctg taggcgtggc caccttcgct cccgcgactg
     8581 atcacgacaa caagcatctt atcgagcgtg ccgaccaggc actgtatgct gcgaagcacg
     8641 caggcagaaa ccgggtcgaa atcaagatag cgtaaaagct tcgggttgcc gtggctgacc
     8701 tgaaaaccat tacgttgaaa aaaaccacgg cttttgccct ggcaatatct gcgtggatcc
     8761 tgattgcgct actcgacagc cagatcacat tcgaaatcgg tttttctatc ttttacctta
     8821 tcccaatatc gctcgccacc tattatggcg gttttcgcca gggtctcgtt atctccgtac
     8881 tatcggcgct ggcctggggc ttcaatgact ggtacacagg tcaccgttac acaaatcact
     8941 ggatgccgct ctggaacata ttcatgcgcc tgggttacta cattctgcat agttatatgc
     9001 tgcgccggta cacaacactg ctggcttaca cgagttcgct tgcttatacc gataccctta
     9061 ccggggcttt caacacgcgc tacctcgcaa ggcacatcgc gcgattgcgc gaaaccggac
     9121 gcacggcaaa acagcctgtt accctggcct atatcgacct cgataacttc aaggaagtga
     9181 atgacaccca cggccatgat gaaggcgacc aggtgctccg agtgctcgct gaagaaaccc
     9241 gcaaaacgat aggcgagaac gacatttttg cccggctcgg tggtgacgag ttcgtggtcg
     9301 tgattcccga aagagatttc actgcagcga agcaaatcat cgagcagctt gccatgaacg
     9361 tgctggcggc ttttcgccgg cgcggttggc ctgtcaccct aagcataggc gcactaacct
     9421 acaaccacgc cgcgaaaata ccggtgtctc cgatgcagca ggttgaccag ctgatgtaca
     9481 aggttaagaa gaccggcaaa gccgcaattc ggcaccagaa ggcataattg ccccatctgc
     9541 ggcccgaatc gacataaact tgacggcaga ttttcattcc tgagtcaagc gcatggctga
     9601 actcagcaaa gacatcgtgg gccgcaagat ggagcccttc acctttaccg ttgaacgcgg
     9661 caaggtaaaa gaattcctca acgctatcgg tgaaacgaac gcgatttatc atgacgtgaa
     9721 tgcagcgaaa gcggccggtt acgccgacac tcccattccg ctcaccttcc agacagcttt
     9781 taccttctgg ggttacggcg ataagttctg gtctgatctc gacagcttcg gtatcgatac
     9841 caagcgcctg ctccacatga aagaagagta tacctacctc gagccgattt accccggcac
     9901 gacggtgagc tgccaggtcg aagttatcga tgtaaaagtc ggcaaaatga acatggtgac
     9961 ttttaagaac acctacagtg acggcaaagg caagatcttc atcgaagccg aaatggcgat
    10021 cgtgatccgc cccgaaggaa tgtaagagag gaaatcatga acaaaatcaa attcgctgac
    10081 tacaacgaag gccaggatat tcccgagctg cgcgtaggtc ctatcaagca gatggatctc
    10141 gtgcgttacg cgggtgcgtc gggcgacttc aacccgatac acaacgacgt cgagttcgct
    10201 aaatcagccg gcctgccagg cactatcgca cacggtatgt acatcatggc tctcatgggc
    10261 cgtcaggtaa ctaactgggc acctccaaca caggtaaaat tctttggcgt caagttcaaa
    10321 ggtatgagcc ttccgggcga aactatggta ttcaccggta aaatcaaaaa gaagaaagaa
    10381 gagaacggcg acaagcttct catggtatcc cttacagcag cgaatgacaa gggcgaagtc
    10441 aaggtcagcg gcgacctgac ggtaaaagcc gaatagttcc gcaggattaa gatggatatt
    10501 gcatttgcag atattggcag ccacaaagtt gtgaaggtaa tgggcgacgt tgacttgtac
    10561 aacgtcggtg acctcaagcg ctcagtgttt gaactgatcg acgaaggcga agttgagtcg
    10621 ctgattattg acatgagcag cgtcaactac atcgactctt ccggcgtcgg cgcgcttgtt
    10681 gctgcccaga agaagatgaa gacccagggc ggcaaatttg gtctgctggg cctcaccgaa
    10741 gatgtgctga atatcctcaa gctcgccaca ctcgaccagt tcttcaagat ttacgaaacc
    10801 gaagcgcagc ttccacaata acgtcgtgcc ggataaccgg cacccttttc tactcttagg
    10861 ctatctatga aaaccgtatt acttgtttat ggcggccagt caggcgaaca cgaagtctcc
    10921 tgtgtttccg ccgcctacct ggaacagacc atcggcgagg ctggctacac tccggtgccg
    10981 gtctatgtcg atcgcacggg agtctggcac tggcagcaga aagttcacaa gaccccagca
    11041 gacaatgcgg cgaatccggc gacactcagc cgcgtcggca gcaatgttgt tttgcgcagc
    11101 gcagcgagct cggtggctgt gcaggtcgct ttcccgatca tccacggcac agccggcgaa
    11161 gacggttcgc tgcaggggta tttcgaagtg ctcggtttac cgtacgtggg cgcgggtgtt
    11221 gccagctctg cggtctgcat ggacaaggcg ctgatgcgcg cgctgtttgc cgtgaatggc
    11281 attccgcagg tgcagtactt tgtcatcaca cgcgctgaca aggctgaccc gcgccaggtc
    11341 gacaagcaga tcagcgagtc ctttggttac ccggttttta tcaaaccctg caacatgggt
    11401 agctcggtgg gagttcataa ggtcagcaat gcatctgccc tcgccgctgc aatttccgat
    11461 gctgctcgct ttgacgacca cctgctctgc gagcaggggc tatatgtgcg cgaactcgaa
    11521 atcgcgatcg ccggcaatta tcccgagtac ctgacttcgg gcgtgggtga gatcaaagta
    11581 aaccacgagt tctattcgta tgaagccaag tacctcgacc caaaaggtgc agaccttttc
    11641 atcaaggccg agatcagtga agcccaggct gcagacatac gcaagctggc ggaacaggct
    11701 tttgcctcgg tacggggcga tggatttgcc cgcatcgact tctttctcga taaagattcg
    11761 ggaaaactct acctcaatga aattaacacc ttaccaggtt ttactcccat cagcatgttc
    11821 ccgcagctct ttcgcgccgc cggggtaagc ggcgctgata tcaccaaacg cctgatcgaa
    11881 tggggagaag cgcgctgtca acgcctgcag cggctgcggg cggcaagccg ctaagaccgt
    11941 gccgggcgac tacacactct cttaccattt cttcggcatc aacgatgacc ggaaccattt
    12001 tgagctcttt atcgacgccg atggagcatc tgaccttgcc acgtggcagc accacccgcg
    12061 cgcagccggc gcgaaacatg accgccgctg gcgctttcgc agggccaaag cgcaccggcg
    12121 tgtctacctg ggtttttcgg ggcccgtgag caacaaccgc ggccgcctgc gcatcttgcg
    12181 acagggcaag gtgcatgaca ggcgtaagac cggcccgcag gattcaattc tggtgacact
    12241 gtgatagcac gccgaaagac gacgcc
//
LOCUS       DAOQJF010000051        12207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2592_length_12207_cov_4.861504, whole genome shotgun sequence.
ACCESSION   DAOQJF010000051 DAOQJF010000000
VERSION     DAOQJF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2592_length_12207_cov_4.861504"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLF85_04330"
     CDS             <1..343
                     /locus_tag="PLF85_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18916.1"
                     /translation="WAEEKYGKRLVGGSDADKNLENYLNTQAYMNVRQNRYNLPVGNV
                     HRLIIEQAFYTYLIRFRSGYDKMTTSNQGIWRNVVTWAMQNGHAAMALVNEKAVMDLR
                     KCRNSPTCDNT"
     gene            complement(385..1269)
                     /locus_tag="PLF85_04335"
     CDS             complement(385..1269)
                     /locus_tag="PLF85_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18917.1"
                     /translation="MKQKHYLVVGASSVAGQKALEAIRLKDPNARITVTTSKAPASGS
                     TALTDRETIYGVDLAKPGTIRHIKPALEAPVDVMVFCPAFGMVGYPAELAPVADVEAC
                     FDFSVRPVLELIDELNPQLTVGFSSYYWLRSLEIAYGSMAFAKYALEKLAVENPNHIR
                     IVRAGLFPSKSLRGICLLIQRGVQKESFPGSTQLKTDWKASGLSFADYFTQFAQGYEK
                     ADLARYFTAPYRGTEEQDLVRAIGAALEPNAQGIINVVGDSLWQESELKHVPDFMVRN
                     KEAFRLAESFEFRHQNIA"
     gene            complement(1482..2066)
                     /locus_tag="PLF85_04340"
     CDS             complement(1482..2066)
                     /locus_tag="PLF85_04340"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNE18918.1"
                     /translation="MIAKTTREKIIDDCLALLWRKGVNGTSMSDIAERAAILKGSFYN
                     YFKSKEEFVSAVLDSYAAKWETNVLAIFRERQLSTRAKLKAYFDRMKELAASMHYTQG
                     SLVGNFAQELSGASEKFAEQAEKIFRRMQASIAQTLREAQAQGALAKDEDPEMLAELI
                     LNSTEGAILRAKTARSTRPIEILEEFLMQKLSFA"
     gene            complement(2145..2678)
                     /locus_tag="PLF85_04345"
     CDS             complement(2145..2678)
                     /locus_tag="PLF85_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18919.1"
                     /translation="MTRKLLLPVAALCVFVRLSPLPTVFANEEIRIEFRSAFAVRDKP
                     GVTHYPRHLPQPLRSVDFCPWQPQPPDVQQVMRPESGYLCELHILAAQAETQALEQIT
                     AAHAGDTRKYARNLRFETTDIGSHRVIRWRLQMGKTRLDHFLLIGKRYNYLFVSSPYG
                     SHGALEKVIEEAKFFPN"
     gene            complement(2724..3932)
                     /locus_tag="PLF85_04350"
     CDS             complement(2724..3932)
                     /locus_tag="PLF85_04350"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE18920.1"
                     /translation="MTEGSFEVIPMLIFFYLNTFLTLLIGNMFTYAMIIYSRAVTGSD
                     AVTGLVYAGNLVPPLILGMYAGTVIDRFDRKRVVMIAQTTFIYTGGAMVFLTAQQMFA
                     VSDFFLIAVMLVNGIGLSFIIPGRMALLGDLFDQQHIPRESMKIHIMIMVGFGLAPFV
                     VGYLRQHFDWYVVFATIGILYGVAMLFLFPLQTRPHKRKHEGESAWHSLVAGLRYVRR
                     EPLILSLLAATFMGLFLVGPLQVLLPEFSKTQMGLNESQRGSLMTILGVGLLIGGGLA
                     QFLAHRMRRGLMIVLGAVASGCSMLLIPWLASPAPVALSLALSGIFGGLMGTLIPAAI
                     QQATVEAARGRVMSIYNIVFQGSVAVAAIGLSRLSKFTSPAFAFEVGGWFIIVGALSC
                     LWLKPLRTLR"
     gene            4085..4726
                     /locus_tag="PLF85_04355"
     CDS             4085..4726
                     /locus_tag="PLF85_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18921.1"
                     /translation="MNKEDIAESIKELLVSETDMSYLAELPEKPLQQLYADLQTYMKR
                     LDENQKPVFKAIAISTSFIPNFIIAKLAHDYFTPYIVAQVCEYMDPKAAAKIGKSLRV
                     DYMGQVAVNGDPKVTARIGDCMEVDIVVNVVREMVKRSFFLKLGELADLLNENHLKNV
                     MLKINDNNAIFQVGLHMQDEGKIIRLSKAWPAATRTYVVAELQKINHPLASKL"
     gene            complement(4731..5618)
                     /locus_tag="PLF85_04360"
     CDS             complement(4731..5618)
                     /locus_tag="PLF85_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE18922.1"
                     /translation="MGASDWFRVLVPPMFWGLSFVAIRIGLESFSPFTLCAIRFFLAG
                     FPLVLFYRFPPVDWRRLVVYSLFIGVLQYGLMFWAIYLKLPAGMSSVLIQTQVYITIF
                     FAWIIFRERVSLLQIAGFVISAVGLLVIGYDYFGGAEAVGFILILLSALFWSAGNILL
                     KTFRIQDFAGFIAWTSFLSSLPLFVLAFSVEGAPQMMQQVQSASLRSWLAIIFMSVFA
                     TQLAFALFSKMMLKLPAAKVMPFGTLVPVFGLSSNAFFLGERMPSRVLLGAILVLAGL
                     VVAVVLSTLLRSRQTQQRT"
     gene            complement(5628..6758)
                     /locus_tag="PLF85_04365"
     CDS             complement(5628..6758)
                     /locus_tag="PLF85_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNE18923.1"
                     /translation="MITDSTRRYRRKIFCVILTAWSATQPLLSQSQPAPAEPEKKEAT
                     WGMFPLVLPLYTPETRLMVAGGGIFWYNPWPGAPRKRVSELMTFFKASQNQQYGLGII
                     AEAYFLQHRLKIMQNLDAYQQPYVAWGVGALTPTAQEEKFEARGFANRTSAMWLIAED
                     LYFGVMWQSQFDRIASENPSGYLQTVQPFGYDGTRANGPGLHLLLDTRDNAFSPERGQ
                     WFEARIFHSNETFAAKGNFTQTTVDYRYFRRLWFGHVLAFNAYLTTTFGDVGWYAMPR
                     LGGQFQMRGFFQGRYLDRHLWTTQVDYRLPVWWRFGLVLFTGVGDVTDDLARPLSDRL
                     KISYGSGLRILIDKEQQINMRLDMGFTNQGDANFYFTFKEAF"
     gene            6887..8620
                     /locus_tag="PLF85_04370"
     CDS             6887..8620
                     /locus_tag="PLF85_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE18924.1"
                     /translation="MHNIDLILTLVGGFIAALLLGLFTQRIGLSPIVGYLLAGIAVSP
                     NTPGFVADRHMAEQLAEVGVILLMFGVGLQFHFKELLEVRRIAIPGAIVQSAVATLLG
                     TAIAHAFGWSWAAGFIFGLAIAVASTVVLTRVLVDNNELHTPTGHIAVGWLVMEDLFT
                     VFALVMLPAIFGDKAQESLALSFGVATLKIVALVVVTMVGGGWVIPRFLAYIAQTGSR
                     ELFTLAILAIALGIAVSSTLLFGVSMALGAFLAGMVVGRSDFSLRAANEALPMRDAFA
                     VLFFVSVGMLFNARSLMENPLLVMATLAIILIGKPLAAFVIIVGMRYPVRVGLAVSAA
                     LSQIGEFSFILAVLGENLKILPTQAANILVAAAIISITINPLIFRSAAPLERWLRSFA
                     LFRRMLRFAEPADMRSPLETGVDPETRQAIVIGYGPVGRTVARLLAANGFKPIVIDLN
                     LKTARELKARNLPVVYGDASHPEILKKAHGETADILILSASSIRGGKEIIREARRMNP
                     KIRILARTAYLRDLAGLRDAGADAVFAGEGEVALSITEHILAAFGATPEQIERERLRV
                     HTKLFGTDAKV"
     gene            8778..9881
                     /gene="recA"
                     /locus_tag="PLF85_04375"
     CDS             8778..9881
                     /gene="recA"
                     /locus_tag="PLF85_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNE18925.1"
                     /translation="MAETKETAAQKAEAAEKAKALDSALVQIEKQFGKGAVMKLNADA
                     MKEIPVISTGSLSLDLALGVGGVPRGRIIEIYGPESSGKTTLTLHIVAEAQKLGGMAA
                     FIDAEHALDPSYARKLGVNIDELLVSQPDNGEQALEIAEALVRSNALDVIVIDSVAAL
                     VPKSEIEGDMGQATMGVQARLMSQALRKLTGAIHKTNTVVIFINQIRMKIGVMFGSPE
                     TTTGGNALKFYASVRMDIRRVESLTKGEETIGNHVRVKVVKNKVAPPFRKAEFDIYFN
                     QGISRESSIIEEAVNVGVMAKSGTWFSYNDNRLGQGKENVRQYLVENPKICDEIEAKV
                     RQVHADGTAKKLLAEKEKLPKGAKAAAATEEVA"
     gene            complement(10048..>10379)
                     /locus_tag="PLF85_04380"
     CDS             complement(10048..>10379)
                     /locus_tag="PLF85_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18926.1"
                     /translation="VTLLMMQEYPWNYKSQTYPIHFAFKTFPEAAEKFETMNHFLRNN
                     GVMAVGLNGKKIVRERVLVEAKGNVPPRPRLEDWPGNKDTDPILTREPLPRRLPLEKR
                     YDSATSR"
     assembly_gap    10380..10479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10486..11145)
                     /locus_tag="PLF85_04385"
     CDS             complement(10486..11145)
                     /locus_tag="PLF85_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18927.1"
                     /translation="MKMLLSLVANELRLYQAQRGLSFAFILALSLLGIFRFGIPVDNV
                     SGALLEIFPAVAYLMANLQLLLMAISWESDAYAYRYYAMSGVPLTQLFFAKTLVTFIV
                     QVPLWFFCIVGFLLFFPVDAPTMGTTVALLLTCLPLGAALAPAGQLIAAISQHSTQKN
                     FLAIALFLPLCLPVLIAATGRVSAVLAGLDTLRYDALLVAAALVFLGAGNLLFAYLFE
                     E"
     gene            complement(11142..11786)
                     /locus_tag="PLF85_04390"
     CDS             complement(11142..11786)
                     /locus_tag="PLF85_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE18928.1"
                     /translation="MTQRITKDKTSLHNPTALVFSQLARRFGGFWALQNASGEILSGE
                     ITALTGENGSGKSTLLLILAGILKAHRGTVATAAPGKIHLVSHQVMAYQDLSVLQNLK
                     LAATLSDSPPGDIQPALRYWRIEDLGDKALRTLSRGQLQRFLLSRALLAKPAVLLLDE
                     PFTGLDHVSEARLVDFIRAEATRGAAILFSDHHLTRARALATRSIEMVKGVCGS"
     gene            complement(11783..>12207)
                     /locus_tag="PLF85_04395"
     CDS             complement(11783..>12207)
                     /locus_tag="PLF85_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE18929.1"
                     /translation="QPFSGDHIQFMKSGIMEIPQAFVITKCDAEDLAKRSLYQLQSSI
                     DFLQKVDKDNKPPAIFQTSSVRQTGFSELAEFILSCRPLDQSGELDREAYFLRKEIRY
                     YYGEFGLKRLAHNLDTTAGKGFEERRQVLLDNLAQQQP"
BASE COUNT         2829 a         3404 c         3429 g         2445 t
ORIGIN      
        1 ctgggcagaa gagaaatacg gcaagcgcct ggtgggcggc agcgatgccg acaaaaacct
       61 ggaaaattac ctgaacacgc aggcgtacat gaacgtcagg cagaaccgct acaacctgcc
      121 ggtcggcaac gtacaccggc tgatcattga gcaggcattc tacacctacc tcatccggtt
      181 ccgttcgggc tatgataaga tgaccacatc gaaccagggc atctggcgca acgtcgttac
      241 ctgggcgatg cagaacggcc atgccgccat ggcactggtc aacgaaaaag ccgtcatgga
      301 cctgcgcaaa tgccgcaaca gccccacctg cgataatacc tgaaatattg ctacgggcaa
      361 tgcccgtagc aatggatggg tgactcaggc gatattctgg tggcggaact cgaaactttc
      421 cgcgaggcgg aatgcttctt tgttgcgcac catgaaatca ggaacatgct tgagctcact
      481 ttcctgccag agtgaatcac cgactacatt gatgatgccc tgtgcattcg gttcgagcgc
      541 agcgccgatc gcgcgcacca gatcctgctc ttcagtgccg cggtaaggcg ctgtgaagta
      601 ccgggcgaga tccgcctttt catagccctg cgcaaactgc gtgaagtaat cagcaaagct
      661 caggcccgag gctttccagt cggtcttgag ttgcgtcgaa ccgggaaaac tttctttctg
      721 cacaccgcgc tggatcagca ggcagatgcc gcgcagcgac ttcgagggaa aaagccccgc
      781 gcgcacaata cgaatgtggt tcggattttc caccgcgagt ttttcgagcg cgtacttggc
      841 aaacgccatc gaaccatacg cgatctcaag cgagcgcagc cagtagtagc tcgagaagcc
      901 gaccgtgagc tgcgggttga gttcgtcgat gagctcaagc accgggcgta ccgaaaaatc
      961 aaagcaggct tcgacgtcgg caacgggggc cagttctgca gggtagccca ccatgccaaa
     1021 ggcggggcaa aacaccataa catcgaccgg cgcttcgagc gcaggtttga tgtggcggat
     1081 tgtccccggt tttgcgagat cgacgccgta gatggtctcg cggtcggtga gcgcagtcga
     1141 accgctcgcg ggagccttgg aagtcgtcac ggtgatacgg gcattcgggt ctttcagccg
     1201 aattgcctcg agcgcttttt ggccggcaac gctcgatgca ccgacgacca ggtagtgttt
     1261 ttgtttcatt ttcttctcct gtccccaccc ccggcccctc cccgcatgcg gggaggggag
     1321 tgaggttggg ctttattggt tttttgtgat aagtttgcgt ttgcgtgccc ctcccccgct
     1381 tgcgggggag gaagggaggg ggacacatat gaccattcat tcatattaat gatgtggaat
     1441 tccatggcag ggttggtgcg gctgtgtttt tccatagcta gtcaggcaaa agaaagcttc
     1501 tgcatgagaa actcttcgag aatttcgatc ggccgcgtgc tgcgcgccgt cttggcgcgc
     1561 agaatcgcgc cctctgtgct gttcaaaatg agctccgcca gcatctcggg atcttcgtcc
     1621 ttcgcgagcg caccctgcgc ctgcgcctct ctcagcgtct gcgcaatcga tgcctgcata
     1681 cggcgaaaga tcttttcggc ctgctcggcg aatttctcgc tcgcacccga aagctcctgc
     1741 gcaaaattgc cgaccagcga accctgcgtg tagtgcatcg aagcagcgag ttctttcatg
     1801 cggtcgaagt aggccttgag ttttgcgcgc gtcgaaagtt gcctttcgcg gaaaatcgca
     1861 agaacgttgg tctcccactt ggcggcgtag gaatcgagca ctgcgctgac gaattcttct
     1921 ttgcttttga aatagttgta gaacgaaccc ttgagaatgg cggcacgctc agcaatgtct
     1981 gacatgctcg tgccgttcac gcccttgcgc cacaaaagcg ccaggcagtc gtcgataatc
     2041 ttctcacgtg tcgttttggc gatcacggcg ccacatatga acgattggtc atatagtcaa
     2101 accattcacc atggagccct gcgggggcat ggccggagcc ttccttaatt agggaaaaac
     2161 ttcgcctctt cgatgacctt ttctagagca ccgtggctgc catagggcga agagacgaac
     2221 aggtagttat accgtttgcc gatcagcaga aaatggtcca gccgggtctt gcccatctgc
     2281 agcctccagc gtatgaccct gtgcgagccg atgtcggtcg tttcgaaccg caggttgcgg
     2341 gcatacttgc gggtgtcacc tgcgtgcgcg gcagttatct gctcgagggc ctgcgtctct
     2401 gcctgcgccg ctaaaatatg gagttcgcac aggtagccgc tctctggccg catcacctgc
     2461 tgcacatcgg gtggctgtgg ctgccaaggg caaaaatcca ccgaacgcag cggttgcggc
     2521 aggtgcctgg ggtagtgcgt gacgcccggc ttatcgcgaa cggcgaaggc gctgcgaaat
     2581 tcgatgcgga tctcttcgtt ggcaaagact gtcggcagag gggaaagccg gacgaacaca
     2641 cacaaggcgg caacgggcaa aagcagctta cgggtcatgc ggtggcgata gccgcggcaa
     2701 cgcccggcgt ctattctttt ctgtcagcgc agtgtgcgca ggggtttgag ccagagacac
     2761 gagagcgctc ccacgataat gaaccagccg cccacctcga acgcgaaggc ggggctcgtg
     2821 aatttcgata gccgtgaaag accgatcgca gcaaccgcaa cagaaccctg gaagacaatg
     2881 ttgtagatac tcatcacgcg gccacgtgct gcctcgactg tcgcctgctg tatggcggcc
     2941 ggtatgagtg tgcccatgag gccgccaaag attcccgaga gtgcaaggct cagcgccacc
     3001 ggcgcgggcg acgcgagcca gggaatcagc agcatcgaac aacccgaggc gactgcgccc
     3061 agcacgatca tcaggccgcg gcgcatgcgg tgcgcgagaa actgcgcgag gccaccgcct
     3121 atcagcaggc ccacacccaa gatggtcatg agcgagccgc gctgcgattc gttgaggccc
     3181 atctgtgttt ttgagaattc gggcagcagc acctgcagcg gccccacaag aaagagcccc
     3241 atgaaagtcg cggcaagcag cgacaaaatg agcggttcgc gccgcacata acgcagcccc
     3301 gcaacgagac tgtgccaggc actttcgccc tcgtgcttgc gcttatgcgg gcgcgtctgc
     3361 agcggaaaaa gaaacagcat ggcaacgcca tagagtatgc cgatggttgc aaagaccacg
     3421 taccagtcaa agtgctggcg caggtagccg accacaaagg gagcgagccc aaaaccgacc
     3481 atgatcatga tgtggatctt catgctctca cgcggaatgt gttgctggtc aaacaagtcg
     3541 ccgagcagcg ccatgcggcc gggtatgata aaactgagac ctattccatt cacgagcatg
     3601 actgcgatga gaaaaaaatc cgacacggcg aacatctgct gcgccgtgag aaaaaccatc
     3661 gccccaccgg tgtaaatgaa cgtcgtctgc gcaatcatga ccacgcgctt gcggtcaaag
     3721 cggtcgatga cggtgccggc gtacataccg agtataagcg gtggcacaag gttgccggcg
     3781 tacactaggc cagttaccgc atcagagccg gtgaccgcgc gcgagtagat gatcatcgca
     3841 taggtaaaca tattaccaat caacagtgtc agaaacgtgt tgaggtagaa gaagatgagc
     3901 ataggtatga cctcgaagct gccttcagtc ataccggcta tggaattcca tgacccccgg
     3961 taggggtagg tttacctgtg gtgagcttgt cgaaccaaac ctacccctac cgggggcacc
     4021 ttctttttaa tattgacgct ttgttcacct ttatttccct ccgcgagcac cttttggagg
     4081 aaccatgaac aaagaagata ttgccgaaag catcaaagaa ctgctggttt cagaaaccga
     4141 tatgagctac ctcgccgaat tgccggaaaa gccgctgcag cagctctacg ccgacctgca
     4201 gacatacatg aaacgactgg acgaaaacca gaagccggtc ttcaaggcga tcgcgatttc
     4261 cacgagtttt attcccaatt tcattattgc caaactcgcg cacgactatt tcaccccgta
     4321 tatcgtggca caggtttgcg aatacatgga ccccaaggca gctgctaaaa tcggtaagtc
     4381 gttgcgcgtc gactacatgg gccaggttgc ggtgaacggc gaccccaaag tgaccgcccg
     4441 gattggtgac tgcatggaag tcgacattgt cgtcaacgtc gtgcgcgaaa tggtcaaacg
     4501 ttcattcttt ttgaaacttg gtgaactcgc cgacctcttg aacgagaacc acctgaagaa
     4561 cgtgatgctc aagatcaacg acaacaacgc catcttccag gtcggcctgc acatgcagga
     4621 tgaaggcaag atcattcgcc tctcaaaggc atggcctgct gcgacacgca cttacgtcgt
     4681 tgctgagctg cagaagatca atcacccgct ggcgagtaag ctttaacggc ctaggtacgc
     4741 tgctgcgtct gccggctgcg cagtagcgta ctgagcacca ccgccaccac caggcccgca
     4801 aggacgagta tcgcgccaag cagaacgcgc gagggcatgc gttcgccgag gaaaaaagca
     4861 ttgctgctca gcccaaatac cggcacgagc gtgccaaacg gcatcacctt ggccgcgggc
     4921 agtttcagca tcatcttgct gaacagggcg aaggcgagct gcgtcgcaaa gactgacatg
     4981 aagataatcg cgagccacga gcgcagcgag gcggattgta cctgctgcat catctgtggc
     5041 gctccttcga cactgaacgc cagcacgaac agcggcaacg acgacaagaa gctggtccag
     5101 gcgatgaagc ctgcaaagtc ctggatgcga aacgttttca gcaggatgtt gccagccgac
     5161 cagaagagtg cagacaacag aatcaatata aatcccactg cttcggcgcc gccgaaatag
     5221 tcgtagccga ttaccagaag gcccaccgca gaaatcacaa agccggcaat ctgcaaaagg
     5281 ctcacgcgct cgcgaaagat gatccaggcg aagaagatcg tgatatagac ctgtgtctgt
     5341 atcagtacag acgacatgcc cgcgggcagc ttgaggtaga tcgcccagaa catgaggccg
     5401 tattgcaaca cgccgatgaa gaggctgtag accaccaggc ggcgccagtc aaccggcgga
     5461 aagcggtaga acagaaccag cggaaagcct gcgagaaaaa aacggatcgc gcagagcgtg
     5521 aaaggcgaga acgactcgag cccaatgcgt atcgccacga agctgaggcc ccagaacatg
     5581 ggcggtacga gtactcgaaa ccagtctgaa gcgccgatgc gggagaatca gaatgcttct
     5641 ttgaacgtaa aataaaaatt agcgtcgccc tggttggtga accccatgtc gaggcgcata
     5701 ttgatctgct gctctttatc gataagaatg cgcagaccac tgccatacga aatcttcaac
     5761 cggtctgaaa gcggccgcgc gaggtcatcg gtgacgtcgc cgacacccgt aaagagaacg
     5821 aggccaaagc gccaccacac gggcaggcga tagtcaacct gcgtcgtcca caggtggcgg
     5881 tcgagatagc gcccctggaa aaagccgcgc atctggaact ggccacccag ccgcggcatc
     5941 gcataccagc cgacgtcgcc aaaggtcgtg gtgagatatg cgttaaatgc caggacgtgg
     6001 ccaaaccaca gccggcgaaa ataccggtaa tctactgttg tctgcgtaaa atttcccttt
     6061 gcggcaaaag tttcgttgct atgaaaaatc cgcgcttcaa accactgccc acgctcgggg
     6121 gagaaagcgt tgtcacgcgt atcgagcagc aggtgcagcc ccggcccgtt tgcgcgcgta
     6181 ccatcgtagc cgaagggctg cacggtctgc aggtaccctg agggattttc actcgcgatg
     6241 cggtcaaact ggctctgcca catgacaccg aaatagaggt cttcggcaat gagccacatc
     6301 gcgctcgtgc ggttggcaaa cccgcgcgcc tcgaactttt cttcctgcgc tgtcggagta
     6361 agagctccca cgccccatgc gacgtagggt tgctggtacg cgtcgaggtt ctgcatgatc
     6421 tttagccggt gctgcagaaa gtacgcttcg gcaataatgc ccaaaccgta ttgctggttc
     6481 tgcgatgcct taaaaaatgt catgagctcg ctcacgcgct tgcgcggtgc accgggccac
     6541 gggttgtacc agaaaatgcc gccgccggca accatcagcc gtgtttcagg agtatagagc
     6601 ggcagcacaa gcgggaacat accccacgtg gcttctttct tttccggctc agctggcgcc
     6661 ggctgtgact gggaaagaag tggctgcgtc gcagaccacg cggtaagaat aacgcagaaa
     6721 atttttcttc gatagcggcg cgtagaatcg gtgatcatga tttgcagtgg aagagatgct
     6781 gcaaatttgt ttcaggccga aaagccgttt gggtaacaag gacctgaaat aaaaaaactc
     6841 cttgtaatct gccacagtat gctgtatatt cactgagcga tacacgatgc acaatattga
     6901 cctcatactc acgctcgtcg gtggtttcat cgccgcactt ctgctagggc tcttcaccca
     6961 acgcattggc ctttctccaa tcgttggcta cctgcttgcc ggtattgcag tcagccccaa
     7021 cacgccgggc ttcgtcgccg acaggcacat ggccgagcag ctggccgaag tcggcgtgat
     7081 cttgctcatg ttcggggtcg gcctgcagtt tcattttaaa gagctgctcg aagtaaggcg
     7141 tatcgccata cccggcgcga ttgtacagag cgccgtcgct acactgctcg gtaccgccat
     7201 cgcgcatgcg ttcggctgga gctgggcagc gggtttcatt tttggtctcg cgatcgctgt
     7261 ggcgagtacg gtggtgctga cgcgcgtgct cgtcgacaac aacgaactac acacacccac
     7321 aggtcacatt gccgtgggat ggctcgtgat ggaagacctc ttcacggtct ttgctctcgt
     7381 catgctgccg gcaatcttcg gcgacaaagc tcaggaaagc ctcgcgctgt cattcggcgt
     7441 ggcgacgctc aagatcgtgg cgctcgtggt cgtgacgatg gttggcggcg gctgggtaat
     7501 tccgcgtttc ctggcgtaca tcgcgcaaac gggttcgcgc gagcttttca cgctcgcgat
     7561 tctggctatt gccctcggca tcgcggtcag ctcaacactg ctgttcggtg tctcgatggc
     7621 actaggtgca tttcttgcag gcatggtagt cggccgttcg gacttcagct tgcgcgcagc
     7681 aaatgaggcc ctgccgatgc gcgatgcgtt cgccgtgctc ttctttgttt ctgtcggtat
     7741 gctttttaat gccaggagcc tgatggaaaa tccgctgctg gtaatggcaa cgctggcgat
     7801 cattctcatc ggtaaaccgc tggcagcctt tgtgatcatc gtcggcatga ggtacccggt
     7861 gcgcgtggga ctggcagtct ctgctgcgct ttcgcagatc ggggagttct cgttcatact
     7921 cgcggtactc ggcgaaaacc tgaagattct gccgacacag gctgcgaata ttctcgtggc
     7981 tgctgccatc atcagtatca ccatcaatcc ccttatcttt cgcagcgccg caccgctcga
     8041 acgctggctg cgctcgttcg cactctttcg tcgcatgctg cgctttgccg agcccgccga
     8101 tatgcgctcg ccgctcgaga ccggcgtcga ccccgagaca cggcaggcga tcgtcatcgg
     8161 ttacggtccc gtcgggcgaa cggttgcccg cctgcttgct gccaatggtt ttaagccgat
     8221 tgtcatcgac ctgaacctga agactgcgag ggagcttaag gcgcgtaacc tgcctgtcgt
     8281 ctatggcgat gccagccacc cggagattct gaagaaggcg catggcgaga ctgcagacat
     8341 tctgattctc agtgcttcga gtatacgcgg gggcaaagaa atcattcgcg aggcgcgccg
     8401 catgaacccg aaaatccgca ttctcgcgcg taccgcttat ctgcgcgacc ttgcagggct
     8461 gcgcgatgcc ggggccgacg cggtttttgc cggtgaaggc gaggtcgcgc tctcgattac
     8521 cgaacacatt ctggcagcgt ttggtgcgac gccagagcag atagagcgcg aacgcctgcg
     8581 cgtgcatacg aaactcttcg gtaccgacgc taaagtgtaa tttttttctc acacctgaga
     8641 aaaaaacgct gtttcctggt atttaatatg tagagagcca tatggttgtt gcggtgtaaa
     8701 ccgcgcacca cgaatggcca caaggagaca taataatgat tgacacaacg ggaaaaaacg
     8761 gcagcgtcac gcccataatg gcagagacaa aagaaacagc ggcacaaaaa gcagaggccg
     8821 cagagaaagc aaaagcgctc gattcggcgc tggtccagat cgaaaaacaa tttggtaaag
     8881 gcgcggtgat gaaactgaac gccgacgcaa tgaaagagat tccggttatt tctaccggct
     8941 cgctctcgct cgacctggca ctcggcgtgg gtggagttcc acgcggacgc attattgaaa
     9001 tctacggccc ggaatcatcg ggtaaaacaa ccctgactct gcacatcgta gctgaagcgc
     9061 agaaacttgg cggcatggca gccttcatcg atgccgagca cgcactcgac ccatcctacg
     9121 cacgtaagct cggcgtgaat atcgacgaac tcctggtctc gcaacccgat aacggcgagc
     9181 aggcgctgga aattgctgaa gcactcgtcc gttccaatgc gctagacgtg atcgtgatcg
     9241 actcggttgc ggcgcttgtt cccaaatcgg aaattgaagg tgacatgggc caggcaacga
     9301 tgggcgtaca ggcccgcctc atgagccagg cgctgcgtaa gctgaccggc gctatccaca
     9361 agacaaacac agttgtaatc ttcattaacc agatccgtat gaaaatcggc gtcatgttcg
     9421 gttcacccga aaccaccacc ggcggtaacg cgctgaagtt ttacgccagc gtgcgtatgg
     9481 atatccgccg cgttgaatcg ctcaccaagg gcgaagaaac gatcggtaac catgtgcgtg
     9541 tcaaagtcgt gaagaacaag gtcgctcccc cgttccgaaa ggcggagttt gatatctact
     9601 tcaaccaggg catctcacgc gagtcttcga tcattgaaga agcggtcaat gtgggtgtaa
     9661 tggcaaaatc gggcacttgg ttctcgtaca acgacaaccg cctgggccag ggcaaagaaa
     9721 acgtacgcca gtacctggta gaaaatccaa agatctgcga cgaaatcgaa gccaaggtac
     9781 gccaggtaca cgccgacggt acagcaaaga agctgctggc agagaaagaa aaactgccta
     9841 aaggcgcaaa agccgcagcc gctacagaag aagtagcata atacatactt tctgctacgg
     9901 gcttgtttcg gcaagttttg ctagcagata ctgcgtcgca gcaagacagt taaggccatt
     9961 cgtcacactg agccagtcga agtgtgacat tgcaaaaacc aacggttaac gagtcgtact
    10021 tcgacgagct cagtacgacg ctattaacta gcgcgaggtc gccgagtcat atcttttttc
    10081 gagcggcaac cgtcgtggca atggctcccg cgtcaaaatc ggatctgtat ctttatttcc
    10141 gggccaatct tcgaggcgcg gacgcggcgg tacattgccc ttcgcctcta cgagcactcg
    10201 ctccctgaca attttcttgc cgttcaggcc gacagccatc acgccattgt tacgcagaaa
    10261 atggttcata gtttcgaact tttctgcagc ctcgggaaaa gttttgaacg caaagtgtat
    10321 cgggtacgtc tgggatttat agttccaggg atactcctgc atcatgagca gggtgacccn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgcccttatt cttcaaacag
    10501 gtaagcaaaa agcaggttgc ccgcccccag gaacaccagc gccgctgcta cgagcagggc
    10561 gtcatacctc agcgtatcga gcccggccag cactgccgat acccggccgg ttgcagcgat
    10621 caacaccggc aggcagaggg gtaaaaagag cgcaatcgcg agaaagtttt tctgcgtgct
    10681 gtgctgcgaa atggcggcaa tcagctggcc tgcgggggca agcgcagcgc ccagtggcag
    10741 gcaagtcagt agtaaagcaa ccgtagtgcc catggtcggc gcatcgacgg gaaaaaacaa
    10801 caggaaacca acaatgcaga agaaccaaag cggtacctgc acgataaacg tgacaagcgt
    10861 cttcgcaaag aacagctgcg tcagcggcac cccgctcatc gcgtagtagc ggtacgcgta
    10921 cgcgtcgctt tcccagctga tggccatgag cagcagctgc aggtttgcca ttaggtacgc
    10981 caccgctgga aaaatttcga gcagggcacc actcacgttg tcgaccggta tgccgaaacg
    11041 gaagatcccc agcagggaca aggcaagaat gaacgcaaac gaaagtccgc gctgtgcctg
    11101 gtagagccgc agttcgttcg ccacgagcga gagcagcatc ttcatgaacc acagactcct
    11161 ttgaccatct cgatcgaacg cgtcgccagc gcgcgcgcgc gcgtgaggtg gtggtcagaa
    11221 aacagtatgg cagccccgcg cgtcgcctcg gcccggataa aatcgaccag gcgcgcttcg
    11281 ctcacatggt cgaggcccgt gaacggttca tcgagcaaca gcacagctgg ctttgcgagc
    11341 agcgcgcggc tgagcaaaaa acgctgcagc tgcccgcgcg acagggtgcg caaggccttg
    11401 tcaccgaggt cttcaattcg ccagtaacgc agcgcgggct gaatgtcacc cggcgggctg
    11461 tcgctcaggg tggccgcgag cttcaggttt tgcaaaacgc tgaggtcctg gtaggccatc
    11521 acctggtgag acacaaggtg gatttttccc ggtgctgcgg ttgccaccgt gccgcggtgt
    11581 gccttcaaaa ttcctgcaag aatcagcagc agcgtcgatt tacccgaacc gttttcaccg
    11641 gtgagcgccg tgatttcacc cgacagtatt tcgccgctgg cattctgcaa agcccaaaag
    11701 ccgccaaagc gccgcgccag ctgtgagaaa accagtgccg tcggattgtg aagagaagtc
    11761 ttatcttttg ttatccgctg ggtcatggct gttgttgtgc gaggttatcg agcaaaacct
    11821 ggcggcgctc ttcaaaacct ttgcctgctg tggtatcaag gttgtgcgcc aggcgcttca
    11881 ggccaaactc accatagtag taacgaattt ccttgcggag aaagtaagct tcacgatcga
    11941 gctcccccga ctggtcgagc ggcctgcatg acagaataaa ctccgccagt tcagaaaatc
    12001 ctgtctggcg caccgagctc gtctggaaaa tcgcaggtgg tttgttgtct ttgtcgacct
    12061 tctgcagaaa gtcgatcgaa gactgcagct ggtaaaggct gcgcttcgcc aggtcttcgg
    12121 cgtcacactt ggtgatgacg aacgcctgtg gaatttccat gatgcccgac ttcataaact
    12181 gaatgtggtc gccactgaag ggctgca
//
LOCUS       DAOQJF010000052        12204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2595_length_12204_cov_5.497736, whole genome shotgun sequence.
ACCESSION   DAOQJF010000052 DAOQJF010000000
VERSION     DAOQJF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2595_length_12204_cov_5.497736"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="hemW"
                     /locus_tag="PLF85_04400"
     CDS             complement(<1..882)
                     /gene="hemW"
                     /locus_tag="PLF85_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNE18930.1"
                     /translation="MSPAATATHTPRLVGVYIHLPYCDVKCAYCDFYSIAARHVDAGF
                     WSGYLDRLQDDLASQVRLLSADTAKPVLASIFFGGGTPSKAPASVIAGVIKAIRSSFP
                     QALSSVEITAEANPESLTRELLAEWREAGLNRVSVGMQSLDEATLHYLGRLYRPQAYR
                     EVLSRIRAAGFNNYSADFITGVPGQSVESTLRDLKFAVGEGVTHLSLYQLTIEPGTLL
                     RQRILRGERPAISDEQQAQQMAAACDWLSAQGFERYEISNFARPGYACLHNRIYWTYR
                     PYLGLGVAAHSFSGRRRF"
     gene            complement(879..1415)
                     /locus_tag="PLF85_04405"
     CDS             complement(879..1415)
                     /locus_tag="PLF85_04405"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNE18931.1"
                     /translation="MTELGKKIDARIRNVPDFPKPGIMFKDITPVIQDAQLFKEVIEA
                     MAQQLKDENLKYVAGIESRGFIFASALATRLGVGMILLRKPGKLPWKTLRATYQLEYG
                     TDAIEIHEDAVAEGDRVAVVDDLLATGGTATAAACLLRDAGADVRQAIFAIELSFLPG
                     REKLRTAGFSQIQSLLTY"
     gene            complement(1433..2416)
                     /gene="rfaD"
                     /locus_tag="PLF85_04410"
     CDS             complement(1433..2416)
                     /gene="rfaD"
                     /locus_tag="PLF85_04410"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNE18932.1"
                     /translation="MIVVTGGAGFIGSAVVAYLNAAGEKDILVVDNLADSAKWKNLVS
                     LKYLDFVHKSQFLARLEAGDFGRPQAIVHMGACSSTTETDGDYLMANNYAYTRMLAEY
                     ALEKDIRFIYASSAATYGGIESGFNDDDATSLTLKPINRYGYSKQIFDEVAIAKGWTK
                     QIAGLKFFNVYGPNEYHKKDMSSVIYKAYYQIGDNGFLKLFKSHRPDFGDGEQKRDFV
                     YIKDCVHVIGQLLQNRSANGIFNLGSGAARSWNELAAAVFSAMGKEKRIEYIEMPAQL
                     IGSYQYFTQASMQKLQAAGISLRQTSLEEGVADYVKNYLMAGELRLQDRQF"
     gene            2532..3236
                     /locus_tag="PLF85_04415"
     CDS             2532..3236
                     /locus_tag="PLF85_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18933.1"
                     /translation="MTFKVLIIEDNHSFIDTLKVMLRDLPLDFSHAYRYNDALAILEK
                     DGVFYNRHENTSEPALATDAPVKKKAAAGSNSLYNENGVFLVIVEQNTETNMKGTDFV
                     THAIRKYPGLSESDFILLTHRLDAVPTRNYGFPVIEKPLRAPQIRQAVLLKIKQAQEI
                     TEAQARAASKPAEPEPAAAEPKAPRKGLRDLLKINRTTEAPAETEAEDQKPVKKARAP
                     KPAAKKAAASKKAKPK"
     gene            complement(3237..3755)
                     /locus_tag="PLF85_04420"
     CDS             complement(3237..3755)
                     /locus_tag="PLF85_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNE18934.1"
                     /translation="MKIIFGVSASVAIYRACDLVRELTKAGHEVHVVMTRTAASWINP
                     VLFEALSDHPVVADATASGMAHIEIRKGAQLMLVAPASADLIARAAHGRADDILTATL
                     LAYDGPRWLAPAMNPSMYAHPATQASLATCQKYGYRILDSANGEAVCGDVGQGKMMAV
                     QDILAAVAAFKA"
     gene            complement(3752..4933)
                     /locus_tag="PLF85_04425"
     CDS             complement(3752..4933)
                     /locus_tag="PLF85_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNE18935.1"
                     /translation="MPKEITHCLFAEKAVHALAASDFPLRRAAGRELLFLFEKQKEAL
                     LFGSVAPDIFYYDIAVPWEKKGPAHGAIWGDLIHGAHGEDTLRHVREMLQVLREPGLQ
                     APLTGGRPLSRDEHSLLLLFVMGYLAHVALDTLMHPFVYYYSGNYYAEDAAEKMRSEA
                     RHRAIETVYDLWNLERFGTTLAKYRPRARIYLRDEPRYLVLGLLALGLLRAWPQITAQ
                     EFGTGELPLDIRQHALFKIAHRSYKKQLLLNKMFQSRSVARFGLWLNARRKDALHANS
                     SLLYPAATYRQYLAKAGKEALVVESFRDYRDPVNNRQLPFSPERIERRILARTQGMYR
                     IVWSYMSGVSSADELNRRLRGFSLNNGRTGVATSAMRHFSPLAIDGNFRFGNQQGVQV
                     S"
     gene            complement(4990..5421)
                     /locus_tag="PLF85_04430"
     CDS             complement(4990..5421)
                     /locus_tag="PLF85_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE18936.1"
                     /translation="MAVADIVKAKAEPMPVIRQSDSVLAAVRQMLSIKETGLLILTPG
                     GDLVGIITEKDVMRLVAERYTQLDKVEVWEVMSKSLKTVSHTATVDEALKLMTEQHIH
                     HLPVMKDDKPAGLISLDDIISTRLRRTEDEARALKDFIAQQ"
     gene            complement(5464..6597)
                     /locus_tag="PLF85_04435"
     CDS             complement(5464..6597)
                     /locus_tag="PLF85_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNE18937.1"
                     /translation="MSVFRERAERKYQVIEDHAKMGGMLKQYAAGKVFYLKGFYERIE
                     VKVLDFKEPNLLIVDSLRDIEGEVTLFYTFNKQLEVIGEVVDKLVNTQYKISVSRVRI
                     ATSDRKSPRHKMSSDQVVINSIRAARNTINASLFNVPTSVKIHLEKFQQRLQDFADEV
                     KVKIFEKDDEKTELVRKTMKILMIHDTQDIMSYIPEDTEGFVDYREHLNTEIGTVMED
                     YRKRKIVSEMIVPIIYLGHDGGSIPLGYIQLISHEKKLGMDTALELKMLAFEMVDQMR
                     DSNTMLISKRQSVGDISRGGMRLVVTDTELKKFLVHQKGFSFDVIFKLQQPITVFTEI
                     VWTAMTPQNDLAIGVSIRGFSSRKGESDRYHQYLDSLAAAKPQ"
     gene            complement(6600..9308)
                     /locus_tag="PLF85_04440"
     CDS             complement(6600..9308)
                     /locus_tag="PLF85_04440"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNE18938.1"
                     /translation="MATKELGSLYNPKGLQEKWYPVWEKEGYFDYHKNTGKQDADLKP
                     EDCYSIVIPPPNVTGSLHLGHALNHTLQDILTRHQRMLGKAALWVPGTDHAGIATQNV
                     VERLLAKEGKRREDMGREKFEERVWQWKAESGGMITHQQRQLGESVNWDMERFTMDEG
                     LSHIVRRVFVQLYNEGLIYRGERMINWCPKDQTALSNIEVEYKEIGGHLYYVRYPLEG
                     AEHEGKKYLIVATTRPETILGDEALAVHPDDERYAHLKGAKAKVPFINKLIPIVFDDS
                     VDKEFGTGVVKITPAHDFNDFATGTRHKLPMTNVMTDSAHMNDLAGPYKGLSREACRK
                     KIVEDLEVAEMIEKIDAHKHSVGHSYRSGAVVEPRLSLQWFVKIRPLADKAVSAVTSG
                     ATQFYPKRWENLFFDWMNKIEDWCISRQLWWGHQVPAWYCNDCEGVTVSMDDATECSH
                     CKSKNIKRDKDVLDTWFSSALWPFSTLMPQEISKIDAIWPGGKVPESDVLKNFYPTST
                     LVTGFDIIFFWVSRMLMMGMHFMDKTPFEKVIIHSLVRDANRQKMSKSKGNVVNPLEK
                     MDEYGTDAFRFFLISIMPDGKDIIYDEARLKGYQAFCNKIWNTARFIMMNTPENYELP
                     KTAPKLSAIDEYVLHELDTAIKKCNEALEGFYFADYALAIYDFLWKTFCDQYIEMSKV
                     ALKDEAMKPGALYTLYTVFRAALKLLHPVMPFITEELNSVLPGNETGLLIVAPWPKAG
                     AHAAATKAENHTDVLLEIAGKIRQVRAELSVEPGAKITVHVAVENSAGADFVRAHSAF
                     LMSLARLGEVKFDKPATKMIHAVLRKGVEIYLDLAGSIDIEREKARLEKEITTIEKGK
                     QAAQAKLDSPQFAERAPTELVEAEKQKLVDYNTRLDRVRDMLSSLS"
     gene            9541..10287
                     /gene="fabG"
                     /locus_tag="PLF85_04445"
     CDS             9541..10287
                     /gene="fabG"
                     /locus_tag="PLF85_04445"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNE18939.1"
                     /translation="MFKDRVAVITGSQRGIGRAIAESLAEKGCNIVISDINAEGAQKT
                     ADEIAAQFGVKAIGVGCNVTKKEEQTALANAAVEKLGRLDIWVNNAGAIRDDLLMRMS
                     EADWDLVQTVNLKGVFFGIQAATKVMMKAKYGKIVNISSVAGLVGSAGQANYASAKAG
                     VIAITKTAAREYASRNITVNAVCPGFILTDMTSNLPDKWKEEVIKQTPLPKRGEPADI
                     AKAVRFLASSDADFITGVILRVDGGMVIGL"
     gene            complement(10683..11588)
                     /locus_tag="PLF85_04450"
     CDS             complement(10683..11588)
                     /locus_tag="PLF85_04450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE18940.1"
                     /translation="MKDTKEFMAAEAVKMNGAAVGVPTRNLQFRHPEEVPRYFAYNGS
                     VVGTTLKAVFSAIFPPGERFFVESVRHFRDQVKDPVLKAKITGFIGQESMHGREHESL
                     NEWFEKRGFDMAFPEAQIKFSLWLCERFSPSQQLAITTLAEHFTAHLAEEWLTHEEFR
                     GSTHAEMLKLWTWHGIEELEHKEVAYDLLQLVSKDEHLERILAVPIILTCMVPGILAS
                     WTYLIAREGKLFDLPRNLSDLWALFKPGGFLMNVLVQMPQFLMKDFHPNQHDTTALVE
                     RYRQEYFSEKGIVAQEYKNRPQAAG"
     gene            complement(11684..12106)
                     /locus_tag="PLF85_04455"
     CDS             complement(11684..12106)
                     /locus_tag="PLF85_04455"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE18941.1"
                     /translation="MALSAEVQKLVTELKLVEHPEGGWYRETYRSPVKLQTEKGERNL
                     ATLIYYLLPAGVESRIHKVSWDEIWLWQGGGVLDVRSYSGDKYQSHLLGHSADALPQQ
                     LMPAGTWFNAVVTEGDYVLAACMVAPGFDFADLTFLPL"
BASE COUNT         2728 a         3400 c         3280 g         2796 t
ORIGIN      
        1 aaaacggcga cggccgctaa aactgtgggc ggcgaccccg agaccgaggt acggccggta
       61 tgtccaatag atacggttat gcaggcaggc gtatccggga cgggcaaagt tggagatttc
      121 gtagcgctcg aacccctgcg cggacaacca gtcgcaggca gccgccatct gctgcgcctg
      181 ctgttcgtcg ctgattgccg ggcgctcgcc gcgcaggatg cgctgcctga gcagggttcc
      241 cggttcaatc gtcagctggt agagcgacag gtgcgtcaca ccctcgccaa cggcaaactt
      301 gagatcgcgc agtgtactct cgacgctctg ccccggcact cccgtgataa aatctgcgct
      361 gtagttattg aacccggctg cacgtatacg tgaaagtact tcgcggtaag cctgcggccg
      421 gtagagcctg ccgaggtagt gcagcgtcgc ttcatcgaga ctctgcatgc ccacgctgac
      481 acggttaaga cctgcctcgc gccactcggc aagcagctcg cgcgtcaggg attcagggtt
      541 ggcttcggca gtaatttcaa cgctggataa cgcttgggga aatgagctgc gtatcgcctt
      601 aattacacct gcaatcacag acgccggcgc tttcgaagga gtacctccgc cgaagaaaat
      661 agaagcgagc acaggcttgg ctgtgtcagc agaaagcagt ctcacctggg acgcaagatc
      721 gtcctgcagc cggtcaaggt agccagacca gaaacccgcg tcgacatggc gcgccgcgat
      781 agaataaaaa tcgcagtacg cgcatttaac gtcacagtaa ggcaggtgga tataaacccc
      841 gacgagccgg ggcgtatggg tcgctgttgc ggcggggctc aatacgtgag caggctctgg
      901 atctgggaaa agcctgcagt cctgagcttc tcgcggcctg gcaaaaagct gagctcaatg
      961 gcaaatatgg cctggcgaac atcggcgcct gcatcgcgca acaggcaggc cgcagcggtt
     1021 gctgtgcccc ccgtggcgag caggtcatcg accaccgcga cgcggtcacc ttcggcgact
     1081 gcatcttcgt ggatctcgat cgcatcggtg ccatactcga gctggtaagt agcgcgcagg
     1141 gttttccagg gcaacttgcc cggcttgcgc agcaaaatca taccaacacc caggcgcgtg
     1201 gcgagagcac tggcaaaaat gaagccgcgc gattcgattc cggcgacgta cttcaggttt
     1261 tcatctttga gctgctgcgc cattgcttca ataacttctt tgaagagctg cgcatcctgg
     1321 ataaccggcg tgatgtcttt gaacataata ccgggcttgg ggaaatccgg tacgttacgt
     1381 atccgcgcgt cgattttttt tccgagctct gtcatataat ttccttaatc aatcagaatt
     1441 gccggtcctg cagtcgcagc tctccggcca tgagataatt tttcacgtag tctgccacgc
     1501 cctcttcgag gctcgtctgc ctgaggctga tacccgcagc ctgcagcttc tgcatgctcg
     1561 cctgcgtgaa gtactggtag ctgccaatca gctgggctgg catctcaatg tattcgatgc
     1621 gcttttcctt acccatcgcc gaaaaaactg ctgcagcgag ctcgttccag ctgcgtgcag
     1681 cgcccgatcc caggttgaag ataccgttgg ctgaccggtt ttgcagcaac tggccgatca
     1741 cgtggacaca atctttgata tagacaaaat ccctcttctg ttcaccgtcg ccgaaatccg
     1801 gccggtgcga cttgaagagt ttaagaaagc cgttatcgcc gatctggtaa taagccttgt
     1861 agatcacgct cgacatgtct ttcttgtgat actcattggg accatagacg ttaaaaaact
     1921 tgagaccagc aatctgcttc gtccagcctt tcgcgatcgc aacctcgtcg aaaatctgct
     1981 tcgagtagcc gtaacggtta attggtttaa gcgtcagact ggtagcgtcg tcgtcgttaa
     2041 aaccactttc gatgccgcca tacgttgcgg cactcgaggc gtagatgaag cgtatgtctt
     2101 tttcgagcgc gtattcggca agcatgcgcg tataagcgta gttgttcgcc attaggtaat
     2161 cgccatcggt ttcggtcgtt gaagagcagg cacccatatg cacaattgcc tgcggccggc
     2221 caaagtcccc cgcttcgagg cgggcaagaa actgggattt atgaacaaaa tcgaggtatt
     2281 tgagagagac aaggttcttc cacttcgccg agtccgcgag attatcgaca accaggatgt
     2341 ctttttcgcc ggcggcgttg aggtatgcca cgacggcact gccgataaac cccgccccgc
     2401 cagtcacgac gatcatggtg cagaaaacag tctcagttca gcgtgcaaag cacaaaggtg
     2461 tattgtcata cgcggtactc cttttgccca aaggcttgtc ggcatgtctg aatctcagta
     2521 agggggtcat aatgacattc aaggtcctta tcatcgagga taaccatagc ttcatcgaca
     2581 cgctgaaagt gatgctgcgc gaccttccgc tcgatttcag ccatgcgtac cggtataacg
     2641 acgcgctggc gatactggaa aaagacggcg tcttctacaa ccggcatgag aatacctctg
     2701 agcctgccct ggcgacagat gcacccgtta agaagaaggc agccgctggt tctaactccc
     2761 tctacaacga aaacggtgtt tttctggtaa tcgttgagca gaatacagaa acaaacatga
     2821 agggtacaga tttcgtaacg cacgcaatcc gcaagtaccc gggcttgagc gaatcggatt
     2881 ttatcctgct cacccaccgt ctcgacgcag tgcccacacg caactacggc ttccctgtaa
     2941 ttgagaaacc gctgcgcgcc ccacagatcc gtcaggccgt tctgctaaag ataaagcagg
     3001 cacaggaaat taccgaagct caggcacgcg ctgcaagtaa acctgctgag cctgaacccg
     3061 ctgcagctga gccgaaagcg ccaagaaaag gcctgcgcga cctgctgaag ataaaccgta
     3121 caacagaagc accggcagaa accgaggcag aagatcagaa accggtaaag aaggcccgcg
     3181 caccaaaacc tgcggcgaaa aaagcggccg ccagcaaaaa agccaaaccc aaataatcag
     3241 gctttgaagg cagcgactgc tgccaggata tcctgtaccg ccatcatttt tccctgcccg
     3301 acgtcgccgc aaaccgcttc gccgttggca gaatcaagaa tccggtaacc atacttctgg
     3361 caggttgcga gcgaagcctg cgtcgccggg tgcgcgtaca tcgaaggatt cattgcgggt
     3421 gccagccagc gcgggccatc gtaagcgagc agcgtggcgg taagaatatc gtctgccctg
     3481 ccgtgcgcgg cgcgcgcaat gagatcggcg ctcgcagggg ccacgagcat cagctgtgca
     3541 cctttacgaa tctcgatatg cgccatgccc gatgcagtag catccgcaac gaccgggtgg
     3601 tcagacaatg cttcaaacaa aacaggatta atccagctgg ccgcggtgcg tgtcatcacg
     3661 acgtgcactt cgtggcccgc cttcgtcaat tcacgcacca ggtcgcatgc ccggtaaatc
     3721 gcaacagaag cgctcacacc aaagatgatt ttcatgatac ctgaactcct tgctggttac
     3781 caaaacgaaa attgccatca atcgcaagcg gggaaaaatg gcgcatcgcg ctggtcgcaa
     3841 cgcccgtccg accattgttg agactgaagc cgcgcagccg ccggttcagc tcgtcggcgc
     3901 tgcttacccc cgacatatac gaccatacga tgcggtacat tccctgcgtg cgcgcgagaa
     3961 tacgccgctc gatgcgctcg ggagaaaacg gcaattgccg gttattcacg ggatcgcggt
     4021 aatctctgaa cgactcgacc acgagggctt cttttcccgc cttcgcgagg tattgcctgt
     4081 atgttgccgc tggatacagc aggctgctgt tggcatgcag cgcatctttg cgccttgcat
     4141 tcagccataa cccaaagcgt gcgacagaac gactctggaa catcttgttc agcagcagct
     4201 gtttcttata agaacggtgt gcaattttga acagggcgtg ctgcctgata tcgaggggga
     4261 gttcccctgt gccgaattcc tgcgccgtaa tttgcggcca ggcacgcagc agcccgaggg
     4321 ccagcagacc gagcaccagg taacgtggtt catcgcgcag gtagatacgt gcccggggac
     4381 ggtacttcgc gagcgttgtg ccaaaacgtt caaggttcca gaggtcgtag acagtctcta
     4441 tcgcccggtg ccgcgcctct gagcgcattt tttcggccgc atcttcggca taataatttc
     4501 ctgagtagta ataaacaaaa ggatgcatca gggtatcgag cgcaacgtgc gcaaggtaac
     4561 ccatgacgaa gagtaaaagc agcgaatgct cgtcacgcga caaggggcga ccgccggtca
     4621 gcggagcttg caggccgggc tccctgagaa cctgcagcat ttcgcgcaca tggcgcaggg
     4681 tgtcctcacc gtgtgcacca tggatcaggt ctccccatat agcgccatgc gcgggccctt
     4741 tcttttccca tgggactgct atatcatagt agaaaatgtc aggtgctacc gaaccgaaga
     4801 gcaaggcctc tttctgcttt tcaaacagga aaagaagctc acgaccggct gcgcggcgca
     4861 gcggaaaatc agaggctgca agcgcatgaa cggctttctc ggcaaaaagg cagtgggtaa
     4921 tttcctttgg catcttttgt cagcgtagcg ccgcggtacc ccgatcaaag ccgggcatcc
     4981 ggaaatctct tattgctgcg cgataaaatc tttgagcgcg cgcgcttcat cttcggtgcg
     5041 ccgcagacgc gtggaaataa tatcatcgag cgaaattaac ccggcaggtt tatcgtcttt
     5101 catcacgggt agatggtgaa tgtgctgttc agtcatcagc ttcagcgcct cgtcaacagt
     5161 cgccgtgtgc gacacagtct tcaggctctt ggacatgacc tcccatactt caaccttgtc
     5221 gagctgggta tagcgttcgg ccacaaggcg catgacatcc ttttcggtga taatgcctac
     5281 caggtcacca cctggcgtca ggattaacaa tccggtttct ttgatggaaa gcatttgccg
     5341 cacggctgcg agcaccgaat cagactgcct gatcaccggc atgggctcag ctttggcttt
     5401 gacaatatct gcgactgcca tagggtactt cagcccgcct ctgcccggac gtaaaggtct
     5461 tttctattgt ggctttgcgg ccgccagcga gtcaaggtac tggtgatagc ggtcgctttc
     5521 gcccttgcgt gacgagaatc ccctgatact gacgccaatc gccaggtcgt tctggggcgt
     5581 catcgcagtc cagacgatct cggtaaaaac ggtaattggc tgctgcagct taaaaatcac
     5641 atcaaaactg aagcccttct ggtggaccag gaatttcttg agttcggtat cggtcaccac
     5701 gaggcgcatg cccccacgcg aaatatcccc caccgactgg cgcttgctga tcagcatcgt
     5761 attcgaatca cgcatctggt caaccatctc gaaggccagc atcttgagct caagcgccgt
     5821 atccattcca agttttttct cgtgggaaat cagctggata taaccaagcg ggatagagcc
     5881 accgtcgtgc cccaggtata tgatcgggac gatcatctcg gaaacaatct tccgcttgcg
     5941 ataatcttcc ataaccgtcc caatttcggt attgagatgc tcacgataat cgacaaaccc
     6001 ttcggtatct tcggggatat aggacatgat atcctgcgtg tcatggatca tcaggatctt
     6061 catagtcttg cgcacgagct cggtcttctc gtcgtctttc tcaaagatct ttactttgac
     6121 ctcgtcggca aaatcctgca ggcgctgctg gaatttctcg aggtgaatct tcactgaggt
     6181 aggaacattg aacagggaag cattgatcgt attgcgcgcc gcgcggatgc tgttaattac
     6241 cacctggtcg gagctcatct tatgccgggg tgatttgcgg tcagaagtgg caatccttac
     6301 gcgggaaacg gaaatcttgt actgtgtgtt gaccagctta tcaacgacct caccgatcac
     6361 ctcgagctgt ttattgaatg tgtaaaaaag agtcacctct ccctcgatat cgcggagcga
     6421 atcgacgatc agcaggttcg gttctttgaa atcgagcacc ttgacttcga ttctctcata
     6481 gaaacccttc aggtaaaata ctttgccagc ggcatactgc ttcaacatgc cgcccatctt
     6541 ggcatgatct tcaatcacct ggtatttgcg ctcggcgcgc tctctgaata ctgacatggt
     6601 caggagagcg aagacagcat gtcccgtact ctgtcaaggc gggtattata gtcgacgagc
     6661 ttctgctttt cggcttctac cagctcggtg ggtgcccgct cggcaaattg cggactgtcg
     6721 agcttggcct gcgcagcctg cttgcccttt tcaatcgttg tgatctcttt ctcgagccgg
     6781 gctttttctc gctcgatatc gatcgagcca gcgaggtcga gataaatttc aacgcccttg
     6841 cgcagcacag catggatcat cttggtggcc ggcttgtcaa atttcacctc gccgagcctt
     6901 gcgagactca tcagaaaagc ggagtgagca cggacgaaat ccgccccggc actgttttcg
     6961 accgccacat ggacggtgat ttttgcacct ggctcgaccg agagttcggc gcgcacctgg
     7021 cggatcttgc cggcaatctc gagcagcacg tcggtgtgat tctccgcttt cgtcgccgcg
     7081 gcatgcgcac cggcctttgg ccacggagca acaatcagca ggcctgtctc attgccgggt
     7141 aacacgctgt tcagttcttc ggtaatgaaa ggcattaccg ggtgcagcag cttgagcgcc
     7201 gcacggaaca cggtatagag cgtgtaaagc gcgcccggct tcattgcctc gtctttgagc
     7261 gcaaccttgc tcatctcgat atactggtcg cagaaggtct tccagagaaa gtcgtagatt
     7321 gccagcgcat agtcggcaaa atagaagccc tcaagcgctt cgttgcactt cttgatcgcc
     7381 gtatcgagtt cgtgcagcac gtattcgtcg atcgccgaga gtttcggcgc agttttgggc
     7441 agctcgtagt tttcaggcgt gttcatcatg atgaaacgcg cagtgttcca gatcttgttg
     7501 cagaatgcct ggtaaccctt gagccgtgct tcgtcgtaga tgatatcttt gccatcgggc
     7561 atgatgctga tgagaaagaa gcggaatgca tcggtaccgt attcatccat cttttcgagc
     7621 gggttgacaa cgttgccttt tgacttcgac atcttctgtc ggttggcgtc gcgcaccagc
     7681 gagtggatga tgaccttttc aaagggtgtc ttatccatga aatgcatgcc catcatcagc
     7741 atgcgcgaca cccagaagaa gatgatgtca aagccggtga cgagagttga tgtggggtag
     7801 aaattcttca gcacatcact ctcgggcacc ttgcccccgg gccaaatcgc gtcgatcttg
     7861 ctgatctcct gcggcatgag cgtcgagaaa ggccagagcg ccgaagaaaa ccaggtatcg
     7921 agcacgtctt tatcgcgctt gatgttcttt gatttgcagt gcgagcactc ggtggcgtcg
     7981 tccatgctga ccgtaacacc ttcgcagtca ttgcagtacc aggcgggtac ctggtgtccc
     8041 caccacagct ggcggctgat gcaccagtct tcgatcttgt tcatccagtc gaagaacagg
     8101 ttttcccagc gcttggggta aaactgcgtg gcgcccgagg tcactgcact cactgccttg
     8161 tcggcgagcg ggcgaatctt tacgaaccac tgcagcgaaa gccgcggttc gaccacagca
     8221 cccgaacggt atgagtgacc gacgctgtgt ttatgcgcgt cgatcttttc gatcatctct
     8281 gccacttcga gatcttcgac gattttctta cggcatgcct cgcggctgag ccccttgtac
     8341 ggccctgcaa ggtcgttcat gtgcgctgaa tcggtcatga cgttagtcat tggcagttta
     8401 tggcgcgtgc cggtcgcaaa gtcgttgaag tcgtgcgctg gagtgatttt cacaacgccg
     8461 gttccgaact ccttatccac tgaatcatca aacacaatcg ggatgagttt gttgatgaac
     8521 gggactttgg ccttggcgcc cttcaaatga gcataacgtt catcgtcagg atgcaccgcg
     8581 agcgcttcat cacccagaat ggtttcaggg cgcgtcgtgg cgacgatgag gtatttttta
     8641 ccctcgtgct ctgcgccttc gagaggatag cgcacgtaat aaagatggcc tccgatctct
     8701 ttgtactcca cctcaatgtt tgacagcgcg gtctggtctt tcgggcacca gttgatcatg
     8761 cgctcgcccc ggtagatgag cccttcgttg tataattgca caaacacacg gcgcacgatg
     8821 tgcgagagtc cctcgtccat cgtgaagcgt tccatatccc agttcacgct ttcgccgagc
     8881 tggcgctgct ggtgcgtgat catgccaccc gattcagcct tccactgcca cacgcgctct
     8941 tcgaattttt cgcggcccat gtcttcgcgc cgcttgcctt ctttcgcgag caggcgttcg
     9001 acgacattct gcgtcgcgat gcccgcgtgg tcggtgccag gcacccacag cgccgctttg
     9061 ccgagcatgc gctggtggcg ggtcagaata tcctgcagag tgtggttcaa ggcatgaccc
     9121 aggtgcaaag agcccgtaac gttaggggga ggtataacaa tactgtaaca atcttccggt
     9181 ttgaggtctg catcctgctt accggtattc ttatggtagt cgaaatagcc ttctttttcc
     9241 cagaccgggt accatttctc ctgcagcccc tttgggttat aaagagagcc aagttctttc
     9301 gtcgccatgc gttaggacgc gattctgagt cagggtgaaa agtagaaccc cctccccctg
     9361 cggggactcc cccttaacag ggggagagca ttacagaaaa ttagcgtaga agaatcttcg
     9421 ctcctcccct gctaagggga ggtggctccg tcttcggtga cagaggggtt aggcccaacc
     9481 actctgtagc aaacgctaca atgtcgcttt accccgcaac gcgtcgatga atttcgacct
     9541 atgttcaaag acagagtagc cgttattacg ggttcacagc gcggcattgg ccgtgcaatt
     9601 gcagagtcac tcgcagaaaa gggatgcaac attgtcatct ctgatatcaa tgccgaaggc
     9661 gcacagaaaa ccgccgacga aatcgctgcc cagtttggcg tcaaagcgat cggcgtcggt
     9721 tgcaacgtca caaaaaaaga agagcaaaca gccctcgcca acgccgccgt tgaaaaactc
     9781 ggccgcctcg acatctgggt caacaacgcc ggcgcgatcc gcgacgacct gctgatgcgc
     9841 atgtcagaag ccgactggga cctcgtgcag accgttaacc tcaagggcgt gtttttcggc
     9901 atccaggctg cgacgaaggt gatgatgaaa gccaagtatg gcaagatcgt gaatatctct
     9961 tctgttgcgg gcctcgtcgg cagcgctggc caggctaact acgcttctgc caaagccgga
    10021 gtgatcgcca tcaccaaaac agcagcgcgt gaatacgctt cgcgtaacat taccgtgaat
    10081 gcagtctgcc ccggttttat cctcaccgac atgacgagca acctgcccga caagtggaaa
    10141 gaagaagtca tcaaacaaac cccactgccc aagcgtggcg aacctgctga cattgcgaaa
    10201 gcagtacgtt ttctcgcctc gtctgacgcg gattttatca ctggcgtgat tctccgcgtg
    10261 gatgggggta tggtcatagg tttgtaaccg caggttacaa gccgttgaga ctgaccgaaa
    10321 gaatcgcgaa ttcgggccct tggcccgaac agaattccgg aaaccgcgaa gcgttcgcgt
    10381 aatgttgcct gaaaggcaac atagcgtttc tggaattctg gcggttcttg caggtcagtc
    10441 taatagttat ttgaatctat gaagtaatac ccagagcgtt ttcgaatttc ttgcggcttt
    10501 tgcgggctga tctaatagca tctcgagtgc gtgagggcgc ttgcctgcaa gcgcttgggc
    10561 gttttgcgtg tgactcgaag agtcacagcg cctgcaattt cttagcgaaa tttgaggcgc
    10621 acgacgaaat attcgtcaaa gttcttggcg gcgcttgcag ggtggtctaa ttgtgcgaag
    10681 agttaaccgg ccgcttgcgg ccggtttttg tattcctgcg ctactatccc cttctctgaa
    10741 aaatactcct gccgataacg ctccaccagt gcggtagtgt catgctggtt cggatgaaaa
    10801 tctttcatca ggaactgtgg catctgcacg agcacattca tcaagaaacc acccggctta
    10861 aagagcgccc acaggtcgct taggttgcgc ggcaggtcaa agagtttacc ctcgcgcgca
    10921 atgagataag tccacgaggc aagaattccg ggtaccatac aggtcaaaat aattggcacc
    10981 gcgagaatac gctcgaggtg ttcgtctttg ctgaccagct gcaacaggtc gtacgcaacc
    11041 tctttgtgtt cgagttcttc gatgccgtgc caggtccaga gtttcagcat ctcggcgtgc
    11101 gtactgccgc gaaactcttc gtgtgttagc cattcttcgg caagatgcgc cgtaaaatgt
    11161 tcagcgagcg tggtaatcgc cagctgctgc gacggcgaga agcgttcgca cagccacagg
    11221 ctaaatttga tctgcgcttc gggaaaagcc atgtcaaagc cgcgcttttc aaaccactcg
    11281 ttgaggcttt cgtgttcgcg cccatgcata gactcctgcc cgataaaacc ggtgattttt
    11341 gctttgagca ccgggtcttt tacctggtcg cgaaagtggc gcaccgattc gacgaagaag
    11401 cgctcaccgg gaggaaatat tgctgagaag actgccttga gggtcgtacc aacgacgctg
    11461 ccattgtacg cgaaatagcg tggaacctct tcgggatgcc tgaactgcag gttacgtgtg
    11521 ggaactccca ccgcggcgcc gttcattttg accgcctcgg cagccatgaa ctctttagtg
    11581 tccttcatga ccgaaatatc ggcaaagacg aacaattcgt caacttttat tgtagcgatc
    11641 gctacagtaa tatcccgctg taggggtagg tttaaaccta cccctacagc gggagaaatg
    11701 tgagatcggc aaaatcaaac cccggcgcga ccatgcacgc cgcgagcacg tagtcgcctt
    11761 ctgtcactac ggcattgaac caggtgccgg cgggcatcag ttgctgcggc agcgcgtctg
    11821 cactgtggcc cagcaagtgg gattgatatt tgtcaccact ataggaccgt acatccaaca
    11881 ctcccccgcc ctgccacagc cagatttcat cccacgacac tttgtggata cgcgactcta
    11941 cgccggcggg cagcaggtaa tagatcagcg tagcgaggtt tcgctcccct ttctctgtct
    12001 gcaatttcac cggagacctg taggtttcgc ggtaccaacc accttcggga tgttcaacca
    12061 gtttgagctc ggtgacgagt ttctggacct cggccgaaag tgccatatca gacggcaaaa
    12121 acaaacgcac tttgcggttt gcggctgttg ggtttttcca gttcgcggtg tttttcgagc
    12181 aggtggtctg ctcccccgcg cttg
//
LOCUS       DAOQJF010000053        12122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2622_length_12122_cov_6.268087, whole genome shotgun sequence.
ACCESSION   DAOQJF010000053 DAOQJF010000000
VERSION     DAOQJF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2622_length_12122_cov_6.268087"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..745
                     /locus_tag="PLF85_04460"
     CDS             254..745
                     /locus_tag="PLF85_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18942.1"
                     /translation="MSLYLTALIIHITCAAIWIGGNVFFVSLIRLLSKRTEFAELRGK
                     IIYAYVMAYRLATYLLFTLVLLSGLYLVHNRGLLNARLWQTTIGQMALAKLLLFTLLM
                     SLQLFHDFIFGPRSFVSEGNRVVMRESHRRSNRMIGQIVFLLSLLMLVAGVLLSRGVS
                     VFG"
     gene            complement(783..1139)
                     /locus_tag="PLF85_04465"
     CDS             complement(783..1139)
                     /locus_tag="PLF85_04465"
                     /inference="COORDINATES: protein motif:HMM:NF025161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HNE18943.1"
                     /translation="MRTQIHLTGKGRGIEIQSDAVLLHTPADALGLIYEHSADLLIVQ
                     KKNVHPDFFDLATGVAGEICQKFVNYQCQLAIVGDYSEITSKSLRDFIFESNRRANVF
                     FPESIETALNESARRA"
     gene            complement(1374..1814)
                     /locus_tag="PLF85_04470"
     CDS             complement(1374..1814)
                     /locus_tag="PLF85_04470"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HNE18944.1"
                     /translation="MKIIGLALLPCLLLGTLHAKDPASYSLRVEVKDLRNSNGILQVN
                     LYNKEDSIPDQKLEKTFKIAKARIVNQSSFVVFKGLPPGRYAVHILHDENSNGKIDKG
                     FVLPVEGVGLSNFESIGLANRPNFSKASFDLKGDKTISVKVIYF"
     gene            complement(1839..2288)
                     /locus_tag="PLF85_04475"
     CDS             complement(1839..2288)
                     /locus_tag="PLF85_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18945.1"
                     /translation="MKVKIITSSVLLAAAMAACHKEPAGAGGHDHHGEATASVGKPVK
                     KFATDDNLRVRMTAILNTMKAMHDAGAKVNLAKTAAKIEATVQDIFKKCKLEPAADAA
                     IHPILAELLKGAELFKKGNEKEGHDTIHKALLRYEDFFDHPGWDHSK"
     gene            complement(2285..2704)
                     /locus_tag="PLF85_04480"
     CDS             complement(2285..2704)
                     /locus_tag="PLF85_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18946.1"
                     /translation="MQGKKNIVIGLFIMAGFMLYGFLLIYLRDFAPNAAEWAAAYGNG
                     KHFEARLAHVHGNLFGFLNILIGYLLFRLDLTAKQKALVSWTGIAGLLMPIGIVMELL
                     LKAPPYFVLVGAVSMTASVFLLGLFVLKSKKVMGDDV"
     gene            complement(2706..3008)
                     /locus_tag="PLF85_04485"
     CDS             complement(2706..3008)
                     /locus_tag="PLF85_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE18947.1"
                     /translation="MNKIESIHDRIVMKSEAKSSTVLFTEGFKVVGLGFMAGQKLAQH
                     SSPSAAFLLVHQGNINFTMGGTTVTMKTGDFIAIPAKEEHEILATEDSRILLTKGV"
     gene            complement(3005..3430)
                     /locus_tag="PLF85_04490"
     CDS             complement(3005..3430)
                     /locus_tag="PLF85_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18948.1"
                     /translation="MTQTETTERGTKPVLRYIIAAVALLFGLVTIKAGGDVLFFNAQA
                     KASAGNYVLFVLWANFVAGFLYVICAAMILLRPALAFNVAAFIAVATAVVFAALGLHI
                     ASGGAYEMRTVYAMVFRTTIWITIAIGLQKINGSSRGKE"
     assembly_gap    3544..3643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3674..4876)
                     /locus_tag="PLF85_04495"
     CDS             complement(3674..4876)
                     /locus_tag="PLF85_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE18949.1"
                     /translation="MISLAGRDIMHSWGKFVFTGVGLGLLIGVTLTMAGVYRGMVDDA
                     MTLLDNSGADLWVVQKDTLGPYAEPSSIYDDTYRSLLSVPGVARASNVTYLSMQVKAT
                     KADVRVMVVGVVPGLPGEPGQPGHLLAGRHITRGHYEAVADIAAGFALGDRVQIRRNF
                     YHVVGLTRRMVSSGGDPMLFIPLKDAQEAQFLKDNDAIQQQRRRTAANPAFNRPGVPG
                     LLQGVLASQSVNPYVNAVLVQIKNGFTPDQVAEPIRRWKRLQVFTRAQMEEILIGKLI
                     ATAAKQIGMFLAILAIVSAAIVAFIIYTLTLGKIREIAVLKLIGTRNITIASMIMQQA
                     LGLGVIGFVVGKTASTFWAPVFPKYVLMLPEDSLRGFIAVLVICALASLIAIRVALKV
                     DPGEAIGG"
     gene            complement(4873..6054)
                     /locus_tag="PLF85_04500"
     CDS             complement(4873..6054)
                     /locus_tag="PLF85_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE18950.1"
                     /translation="MLRKDQTFWQRFRDQLKKIPLPVSRRTALIILGLVPLVGLFVFV
                     VLRSGPLAPVPVTVTTVEVRQVSPALFGIGAVEAKSLFKIGPNAPGRVRNVFVNVGDR
                     VRARQLLAEMERPDLDILNDTDAERQFIKKALQQEESDARERLRLAEKTLRELVARQA
                     SPSEISDAQGAVVSAREDLARAAGSAKNPLRVSANLRLVSPVQGLVTQRSAEPGTTVV
                     AGQAVVEVVDPKSLWINVRFDQVGSAGLSAGLGGKIVLRSQSVPLTGRVLRVEPLADA
                     ITEETLAKVVFDALPIPLPPIGELAEVTIILPSLLPKPTIPNASLVRVAGELGVWALD
                     GSTNKILFKPVKAGAADLEGNVQILEGLQAGDRVVVYSQKPLKAMSRIKIVDKLVGAP
                     R"
     gene            complement(6058..6675)
                     /locus_tag="PLF85_04505"
     CDS             complement(6058..6675)
                     /locus_tag="PLF85_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE18951.1"
                     /translation="MPEGQRKIFEAAVDEFAEHGFAGSSTLAIAERAGVSEGLIFKYF
                     KSKATLLKQVVFPVLATAIMPLAIRGVRSVTDSQHETCGDFLRALVRERLEFARRHQK
                     HLRILLQELPLNEELRNRMMKMLQAELFPVMQQKILHYQKTGELRAMPSEQMLSLIIP
                     QILGFVLGRAVLGLQFGTGDEADLDMLIEVMLQGLHAPNVRGQKR"
     gene            complement(6821..7849)
                     /locus_tag="PLF85_04510"
     CDS             complement(6821..7849)
                     /locus_tag="PLF85_04510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE18952.1"
                     /translation="MMMATYRKYRYFVQAAALTMIVLLFISAVYYFVQRQREDYDTRK
                     AKIRPAPEVSLSDAEGKKHNLKQAIEPGIVVIWASWCGPCKEELRKLEKTDLVDWSYT
                     AINADEKRQDALDFLKKERIRKPLVLFDPESQLVPVQGYPTIYATYGDGTWAGPLTHT
                     DFDSLLKEIRHTLNRTEYKPYKASKAANERFWQSAYWQLFRYVSLTSASLYFAALLLL
                     VWRILPTSRILVAGLIAHFVISTTENLGFLRLADIVQLRGTVGNIISLLYYWPFVWGG
                     IIALGILQYMHREIRQREGAPDPDQKHEPAVLSVLTEAMSQKTPRYKTTKSRRRVPAA
                     KVKKRTGR"
     gene            7977..8711
                     /locus_tag="PLF85_04515"
     CDS             7977..8711
                     /locus_tag="PLF85_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE18953.1"
                     /translation="MIDQLTFTICVVGAHHSMSLFGNVSLAVVYKVNLFPERSLYKER
                     NIPPQHLTKGALRDLLIASLITQPLATWFLLYPVMMWRGMTYLPPVENPLLASLWVVG
                     AVIVAFFLNDTFNYWTHRALHHRWFYKSIHKKHHLFTKDCVGLTSEYNHPIEQVISTL
                     CTLVPIMILGLDLVSTCVFLFFRTWEVLDEHCGYRFPSPFGSEHSRRHDFHHARSGMN
                     NLGMFRFWDWLMGTNKNYKAYLKKIC"
     gene            8795..9256
                     /locus_tag="PLF85_04520"
     CDS             8795..9256
                     /locus_tag="PLF85_04520"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HNE18954.1"
                     /translation="MTYAILKLIHILSATILFGGGICAALIGTLIFGSKNVKLIGAAG
                     AYVLKVEFYLTVVSFAIQVGTGFWMAGIAGFSIFSGWIGMALVLLVIAAICWIPGLWL
                     QHKMAALANEASNSDMALPTKYDTYFRIWTALGLPSTVAMVGIFYLMVLKA"
     gene            complement(9325..10287)
                     /locus_tag="PLF85_04525"
     CDS             complement(9325..10287)
                     /locus_tag="PLF85_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE18955.1"
                     /translation="MKTAYRAHYCQPEQLAVKEVPDPKPAADQLLVRVRATTVNRTDC
                     AVLTAKPFIMRFFIGFFKPKLPVPGTDFAGEIVAVGSSVTQFKPGDRVFGFNDKGLAS
                     QAELMAIAANAPLAKIPHGLSFEQAAASLEAAHYAYNFANKLKLKAGQNVLVNGASGG
                     IGSALVQILKHFGVIVTAVCNGKNVERVRALGAAKVIDYEREDFTRLTEKFDFVLDAV
                     GKSTFGKCKHLLKDKGVYLSSELGPGGQNIFLPLITAPWGGRRVVFPIPGDIPGSIAF
                     ICSLLTSGAFKPVIDRTYPLADIAAAYRYVMTGQKTGNVVILYS"
     gene            complement(10349..10849)
                     /locus_tag="PLF85_04530"
     CDS             complement(10349..10849)
                     /locus_tag="PLF85_04530"
                     /inference="COORDINATES: protein motif:HMM:NF022606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HNE18956.1"
                     /translation="MNNAFEPVLRVLFIGLGATFLMDLWTIFLRAFGIKSLNYAFLGR
                     WIGHLPQGQWIHENISQSTPVRYELLLGWCAHYTIGISFAALLVAVYGLDWLRSPSLL
                     PALVIGVATVAAPYFILQPGLGAGIASGKTATPNFNRVKSLMTHTVYGFGLYFAARLA
                     ACLLPA"
     gene            11041..11406
                     /locus_tag="PLF85_04535"
     CDS             11041..11406
                     /locus_tag="PLF85_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE18957.1"
                     /translation="MQKKGVRKKQEYRHLPAFILLVLTQGPAHGGAVHAALSKKLPAY
                     NADTGAVYRTLLEMEQNGEIEFTWDTSSSGPARKIYQLTPAGWEKLAYWKKDIEGRLE
                     YLNIFLKSYKKIKNPGKKS"
     gene            11425..11841
                     /locus_tag="PLF85_04540"
     CDS             11425..11841
                     /locus_tag="PLF85_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HNE18958.1"
                     /translation="MNTLTAILKEAQKYALSIRPKVGGFPVLAEVLRQAGVHVNRWSL
                     PSCQSIYIMDGGAVVQQGTPLVSGTHEIPKFNREALITALRTDQDGRSTFPEFLQAVW
                     NAGIIGYDADFGRRLVTYYGATGESYTEEYPAVELK"
BASE COUNT         2787 a         3258 c         3074 g         2903 t
ORIGIN      
        1 gtatcgcgga tatgcaggga ctcgaacctt catacccctt ggtgtaattt gtatgaggct
       61 ggtccagggt cttttggact ttgtatagtc cgggctctgc agctatttaa ttgattgcat
      121 aacatggagc cagatgtttt caggcaattg atgccagtca tggccagtta gcgaaagcag
      181 atcattgaca accccaggaa atctcagaag taaatgtgtg acatgccatg ccagctacag
      241 catcaagtag cgcatgtcgc tctaccttac tgcactgatt atccatatca cctgcgccgc
      301 catctggatc ggtggcaacg tctttttcgt cagcctgatt cgactactga gcaaacgcac
      361 cgagttcgcc gagctgcgcg gcaaaattat ctacgcctac gtcatggcct accgcctcgc
      421 gacctatctg ctgttcacgc tggttttact ttcgggtctg tacctggtgc acaaccgcgg
      481 attgctgaac gcacggctgt ggcagacaac cattggccaa atggcgctgg caaaactgct
      541 gctcttcacc ctactgatgt cgctgcagct gttccatgat tttattttcg ggccgcggag
      601 tttcgtcagt gaaggcaacc gggtcgtgat gcgcgagagc caccgccggt cgaaccgcat
      661 gatcgggcag atcgtctttt tactttcgct gctaatgctc gtcgccgggg ttttgctgtc
      721 acgcggggtt tcggtgtttg gttagccgcg atgtctggcc ccgcggcaag aatgaactta
      781 actcatgccc ggcgtgccga ttcgttcaat gccgtttcaa tgctttcagg aaaaaataca
      841 ttcgcacgcc ggttgctttc aaaaatgaaa tcccgcaggc tttttgacgt aatctcagaa
      901 tagtcgccta caatcgccag ctggcactgg tagttcacga acttctggca gatctcgcca
      961 gccaccccgg tagccagatc aaaaaagtca ggatgtacgt ttttcttctg cacgatcagc
     1021 agatcggcag aatgctcata gatcagcccc agtgcatcgg caggcgtatg caacaagaca
     1081 gcgtcagatt gaatctcaat accgcggcct ttgcccgtca ggtgaatctg ggtccgcatt
     1141 tggggtgaag gcgttgtcga agccaaaact tctgtgttta taggtcgcat gctcggtcac
     1201 tttttattgg gtgggtagtc gaatataaag ggattgattt atccggtaaa gctgatttta
     1261 tcggctgagc ctattatgag gatttcttac tggtagctca attcgttgat tggacaaatt
     1321 ttcagttacc agattctccg tgtgtcaggg tggttgtcgc gcacggacgt aggttaaaaa
     1381 tagatcacct tgacggaaat ggttttgtct cccttcagat caaagctcgc cttagagaaa
     1441 ttgggccgat ttgccagacc gatagattcg aaattggaaa gcccgacacc ttcgacgggt
     1501 agcacaaaac ctttgtcgat cttgccattg ctattttcgt catggagaat atgcactgcg
     1561 tatctgccgg gcggcagacc tttgaaaacc acaaacgacg attggttaac aatgcgtgct
     1621 ttggcgattt tgaaggtttt ctccagtttt tggtcaggaa tagaatcttc tttattatac
     1681 aggttaacct gaagaattcc gtttgaattc cgcagatcct tcacttcaac ccgaagtgaa
     1741 tacgatgcgg gatcttttgc gtggagcgtg cccaaaagca ggcacggcaa caacgcaagg
     1801 ccgattatct tcatctcgtc gactctgttt cgaatcggct acttcgagtg atcccaaccc
     1861 gggtgatcaa agaagtcttc atagcgcagc agcgctttat gaatggtgtc atgcccttct
     1921 ttttcattac cctttttgaa tagctcagca cctttcagca actccgccag aatcggatgg
     1981 attgcggcgt ccgctgcggg ttcgagtttg catttcttaa agatatcctg tactgttgcc
     2041 tcgatttttg ccgcggtttt agccagattg accttcgcgc ccgcatcgtg catcgccttc
     2101 atggtattga gtatcgcggt catgcgcacc cgaagattgt catcggttgc gaattttttt
     2161 acgggtttgc caaccgaggc ggtggcttca ccatgatggt cgtgtccccc tgcaccggcc
     2221 ggttctttgt ggcaggccgc catggccgct gccagcaata ctgaacttgt gattattttt
     2281 actttcatac atcatctccc ataacttttt tagattttag aacgaacaac cccaagagaa
     2341 aaacactcgc cgtcatgctg accgcgccga ctaacacgaa atacggcggt gctttcagta
     2401 acaattccat cacaataccg atcggcatca acagcccggc gatgcctgtc catgaaacaa
     2461 gggccttctg ttttgccgtc aaatcgagac gaaaaagcag ataaccgatg agaatattca
     2521 gaaagccaaa caggttgcca tgtacgtgag ccagccgcgc ttcaaaatgt ttgccattgc
     2581 catacgctgc cgcccattcg gcggcattcg gggcaaagtc gcgcagatag atcaataaaa
     2641 aaccgtacaa cataaaacct gccatgataa acaggccaat tacaatattt ttctttccct
     2701 gcattttaca ctcctttagt cagcaaaatt ctcgaatctt ccgtcgccaa tatctcatgc
     2761 tcttcttttg ccgggatagc gataaaatcc ccggtcttca tcgtcaccgt tgtaccaccc
     2821 atcgtgaaat ttatattgcc ctgatgcaca agcaaaaagg ctgccgaggg cgagctgtgt
     2881 tgcgcgagct tttgccccgc cataaagccc aacccaacca ccttaaagcc ctcggtgaag
     2941 agcaccgtgc tggacttggc ctcggacttc attacgattc tgtcgtggat tgattctatt
     3001 ttattcattc ttttcccctt gaagaaccgt ttatcttttg cagaccgata gcgatcgtta
     3061 tccagattgt cgttctgaat accatcgcgt aaactgtgcg catctcgtat gctccacccg
     3121 aggcaatatg caaccccagc gcggcaaaga cgacggctgt agcgactgca ataaaagctg
     3181 cgacattgaa agccagggcc ggcctcagca aaatcatagc ggcgcaaatc acgtacagaa
     3241 agcccgcgac aaaatttgcc cacagcacaa agaggacata attgcccgca ctggcctttg
     3301 cctgcgcatt gaaaaagagc acgtcacctc ctgccttgat ggtcactagg ccaaatagca
     3361 gcgccaccgc tgcaatgatg tagcgtagta ccggttttgt accacgctcc gttgtttctg
     3421 tctgtgtcat tttttctcct tcgccaggat tttatgtccc tggccttttt cttcgagtgt
     3481 tttgccgtcg cggatattat agatgcgttt gaatgtcggg ataatttttt cgtcgtgcgt
     3541 cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcttgc ggagggaagt
     3661 aatgactatt ccattatcca ccaatagcct ccccagggtc gacttttagt gcgacacgaa
     3721 tggcgatcag gcttgcgagt gcgcagatta ccagcaccgc gataaatccg cgcagcgaat
     3781 cttcgggcag catgagcacg tatttcggaa acaccggtgc ccagaaggtt gaggctgttt
     3841 tgccgacgac aaagccgatg acgccgagcc cgagcgcctg ctgcatgatc atcgaagcga
     3901 tcgtaatgtt tcgggtgcca atgagtttga gcaccgcaat ctcgcggatt tttcccagcg
     3961 tgagggtata gataataaag gcgacaatcg ccgcgctcac gattgcgaga atcgcgagaa
     4021 acatgccgat ctgcttcgcc gcggttgcaa tgagtttgcc gatcagaatc tcttccatct
     4081 gcgcgcgggt aaaaacctgt aggcgtttcc agcgacgaat cggttcggca acctggtcgg
     4141 gcgtgaaacc gtttttgatc tgcacgagca ctgcgttgac atacgggttc acgctctgcg
     4201 aggccagaac tccctgcaac aggcccggca ccccggggcg gttaaacgcg gggtttgccg
     4261 cggtgcgccg acgctgctgc tgaatggcgt cgttgtcttt caaaaactgc gcctcttgcg
     4321 catctttgag cggaataaac agcatcggat cgccgccaga cgagaccata cgccgggtta
     4381 agcccacgac atggtaaaag ttgcggcgaa tctgcacgcg gtcgcccaaa gcaaaacccg
     4441 cggcgatgtc ggcgaccgct tcatagtggc cgcgtgtgat atggcgacct gcgagcaaat
     4501 gtccgggctg gccgggttca ccgggcaagc ccggtacgac gccgaccacc atcaccctga
     4561 catccgcctt agtggctttc acctgcatgc tgagataagt gacgttggac gcgcgcgcca
     4621 cgcccggtac gcttaagaga ctgcggtacg tatcgtcgta gatgctcgac ggttcggcgt
     4681 atggccccag cgtatctttt tgcacgaccc agagatcggc gccgctgtta tcgagcagcg
     4741 tcatcgcatc gtctaccatg ccacgataga cacccgccat cgtcagcgtg acgccgatca
     4801 agaggcccag ccccaccccc gtgaaaacaa actttcccca cgagtgcatg atgtcacgac
     4861 cggcgaggct gatcaacgcg gcgctcccac cagcttgtcg acaatcttga tacggctcat
     4921 ggccttcagc ggcttctggc tgtagaccac aacgcggtcg cctgcctgaa gtccttcgag
     4981 tatctgcaca ttgccctcaa gatctgctgc gccggctttg acaggcttaa agaggatttt
     5041 attcgtactg ccgtccagcg cccagacgcc gagttcaccc gcaacacgca cgaggctcgc
     5101 attgggaatc gtgggttttg gcagcagcga tggcagaata attgtcactt cggcgagctc
     5161 gccgatcggc ggcagcggaa ttggcagcgc atcgaacacg accttcgcca acgtttcttc
     5221 cgtgatcgca tcggcgagtg gctcgacgcg cagcacgcga ccggtcagag gcacgctctg
     5281 cgaacgtagc actattttgc cgccaagccc ggcactcagg ccagccgaac cgacctgatc
     5341 aaagcgcacg ttgatccaca gtgatttggg atcaaccact tcaacgacag cctggccggc
     5401 gacgaccgtt gtgcccggct cggcgctgcg ctgcgtaaca agtccctgca cgggtgatac
     5461 cagccgcagg ttcgcgctca cgcgcagcgg gttttttgca ctgccagctg cacgcgccag
     5521 atcttcgcgc gcagatacta ctgcgccctg tgcgtcactg atttctgatg gcgatgcctg
     5581 gcgcgcgaca agttcacgca gagttttctc tgccagtctc agtctttccc gcgcatcact
     5641 ctcttcttgc tgcagtgcct tcttgataaa ttggcgctct gcgtcggtgt cgttcagtat
     5701 atcgaggtcg ggtcgctcca tctcggcgag cagctggcgc gcgcgcacac gatcaccgac
     5761 attgacaaaa acattgcgaa ccctgcccgg cgcattgggg ccgatcttaa aaagcgattt
     5821 tgcctctacc gcgccaatac caaagagcgc gggcgaaact tggcgcactt cgacggtggt
     5881 caccgtcacc ggcaccggag caagcgggcc cgagcgcagc accacgaaga caaaaagacc
     5941 caccagcggc acaagcccca ggataattag cgccgtgcga cggctcaccg gcaaaggtat
     6001 tttttttaac tgatcgcgaa agcgctgcca gaaggtttgg tcttttctca gcattgttta
     6061 cctcttttgc ccacgaacgt tgggggcatg tagcccctgc agcatgactt cgataagcat
     6121 gtctaaatcc gcctcatcac cggtgccaaa ctggagacct aaaaccgcac gacccagaac
     6181 gaagcccaga atctgcggaa tgatgaggct cagcatttgt tctgacggca tggcccgcaa
     6241 ctcgcctgtt ttctggtaat ggaggatctt ctgctgcatc acgggaaaca gttctgcctg
     6301 cagcatcttc atcatgcggt tacgcagctc ttcattgagc ggcagctctt gcagcaaaat
     6361 gcgcaggtgt ttctggtggc ggcgtgcaaa ctcgaggcgt tcgcgcacga gcgcacgcag
     6421 aaagtcgccg caagtttcgt gttgcgaatc ggtgacgctt ctgacgccgc ggatcgcgag
     6481 cggcataatc gccgtcgcca gtaccggaaa gacaacctgc ttcagcaggg tggccttgct
     6541 tttgaagtac ttaaaaatca acccttcgga gacccccgcg cgttcggcga tcgccagcgt
     6601 cgatgaaccc gcaaagccat gttcagcgaa ttcgtccacg gcggcctcga agattttgcg
     6661 ctgcccctct ggcattgtgg ccacggctgt gtcgagctct gcccagctgt ctttggcggt
     6721 tttggactta taccttttct taggggaggc cacagcctta attaagtaag cactcactca
     6781 cccggcgaat atttttgcgc ttctccagct tctggccgta tcaccggcca gtgcgttttt
     6841 taacctttgc tgcgggtacc cgtcgacggg attttgttgt cttgtagcgt ggggtcttct
     6901 ggctcatagc ttcggtcagt acagacagaa ctgcaggttc atgcttctga tctggatctg
     6961 gcgcgccttc gcgttggcgg atttcacggt gcatatactg caaaatgccc agggcaataa
     7021 tgccacccca gacaaacggc cagtaataca gaaggctgat aatattgccc acggttccgc
     7081 gcagctgtac gatatcagca aggcgtaaaa agcccaggtt ttcggtagtc gaaatcacaa
     7141 aatgagcgat taatcccgca accagtatgc gcgaagtcgg taagattcgc cagacaagca
     7201 gcagcaatgc agcaaagtag agcgaagcgg aagtcagaga aacgtagcga aacaactgcc
     7261 agtatgccga ttgccaaaag cgttcatttg cggcttttga agccttgtac ggtttgtatt
     7321 ctgtccggtt cagcgtatgg cggatttctt tgagtaatga gtcaaaatcg gtatgcgtga
     7381 gcggccccgc ccaggtgccg tcgccatagg tcgcatagat ggtcgggtac ccctgaacag
     7441 gaacaagctg agattcgggg tcgaagagca ccaacggttt gcggatgcgt tctttcttca
     7501 gaaaatccag cgcatcttgc ctcttctcat cggcattgat tgccgtgtag ctccagtcaa
     7561 ccagatctgt tttctcaagt ttgcgcaatt cttctttaca gggtccgcac cacgatgccc
     7621 agataacgac gataccaggt tcgatcgcct gtttcaggtt atgtttttta ccttctgcgt
     7681 cgctgagact gacttcaggc gccggacgga tttttgcctt tcgcgtatca tagtcttcgc
     7741 gctggcgctg aacgaagtag tagaccgcgc tgataaacaa cagcactatc atagtaagcg
     7801 ctgcggcctg aacaaaatat cggtacttgc ggtaggttgc catcatcata taccacgcga
     7861 ctccccatcc accatagcgt aaactgcaaa tgcttttttc actgccggca aacagcgcga
     7921 aaaattgaat ttacgttgca gctatcattt actgcgctcg ccgaccggaa caaattatga
     7981 tcgatcaatt aacgtttacc atctgtgttg tcggcgccca ccactccatg agcctctttg
     8041 gcaatgtctc cctggctgtt gtgtacaagg tcaacctgtt tcccgagcgc tctctataca
     8101 aagaacgaaa cattccgccg cagcacctta ccaaaggcgc gctgcgcgat ctgctcatcg
     8161 cctcgctcat cacgcagccg ctggcgacgt ggttcttgct ctaccctgtc atgatgtggc
     8221 gcggtatgac atacttgcct cccgtagaaa atcccctgct tgcatcgctt tgggttgttg
     8281 gtgcagtgat cgtggcattt tttctgaatg atacgttcaa ctactggacg caccgcgcac
     8341 tgcaccaccg ctggttttat aagagcatac acaagaagca ccatcttttc accaaggact
     8401 gcgtgggatt aaccagcgag tacaaccacc cgatcgaaca ggtaatctct actctctgta
     8461 cgctggtacc catcatgatt ctcggtctcg atctcgtgag cacctgcgtc tttctcttct
     8521 tccgcacgtg ggaggttctc gacgaacact gcggctaccg ctttccttcg ccctttggca
     8581 gcgaacattc acggcgccac gactttcacc acgcgcgttc gggcatgaac aacctgggca
     8641 tgtttcgttt ctgggactgg ctcatgggaa cgaacaagaa ctacaaggcc tacttgaaga
     8701 agatttgcta gttcacgata gccagcaaaa tggtcgcgaa tttctgcaat acgaatacag
     8761 cttttcacta aagttacaga ctccggagat accaatgact tatgcaattc ttaagctcat
     8821 ccacattttg agtgcaacta ttttatttgg aggagggatt tgtgcagcac ttatcggaac
     8881 cctgatcttc ggctcaaaga atgtgaaatt gatcggtgcg gcgggtgcat atgttttaaa
     8941 agtcgagttt tatttgacag tggtatcttt cgctattcag gttggtacag gtttctggat
     9001 ggccggcatt gcaggatttt cgattttctc cggttggata ggaatggctt tggtgttact
     9061 ggtcattgca gctatctgtt ggataccagg tctctggcta caacataaaa tggcagcact
     9121 cgcaaatgaa gccagtaatt ctgatatggc attacccact aaatatgata cctattttag
     9181 aatatggacg gctttgggtt taccctccac agtagcaatg gtaggtatat tttatttgat
     9241 ggttcttaag gcatagaatg cacgtaccat gcgtgcggcg tcaacgcttc attgtggctg
     9301 aggccgagca ctcagccaaa attcttagct gtagagaatc acaacgttgc cggtcttttg
     9361 gccggtcatg acgtacctgt acgctgcggc aatgtcggcc agcgggtaag tgcggtcgat
     9421 aacgggtttg aaggcgccgc tcgtcagtaa tgaacagata aatgcgatgc tccccggaat
     9481 atcgccgggt atcgggaaaa caacgcgccg gccaccccac ggtgcggtga tcagcggcag
     9541 aaatatattt tgcccacccg ggccgagttc tgacgaaagg tagacgcctt tgtctttcag
     9601 caaatgctta catttgccaa aggtgctctt gcccaccgca tccaaaacaa aatcaaactt
     9661 ttcggtcagg cgcgtaaaat cttcacgctc gtagtcgatc acttttgcgg cgccaagcgc
     9721 acgcacgcgt tcgacatttt taccgttgca aactgcagta acgatgacac cgaaatgttt
     9781 caaaatctgt accagcgccg aaccgatacc gccgctcgcg ccgttgacca aaacattttg
     9841 tcccgcttta agtttcagct tattggcaaa gttgtaagca tagtgcgcag cttcgagact
     9901 ggccgcagcc tgttcgaaac taagaccgtg cggtattttt gccagcggtg cgtttgcggc
     9961 aatcgccatc agctcggcct gcgaagcaag acccttgtcg ttgaaaccga aaacccggtc
    10021 gccgggtttg aactgggtga cactgctacc gacggccaca atttcacccg cgaagtcggt
    10081 accaggtacc ggcaatttgg gtttaaaaaa gccgatgaag aagcgcatga tgaatggctt
    10141 tgcggtcagt accgcgcagt cggtacggtt cactgtcgtg gcgcgaaccc tgaccagcag
    10201 ctggtcagcc gccggtttcg gatcgggcac ttctttaact gccagctgtt cgggttggca
    10261 gtaatgagct cggtaagctg ttttcatcgg tagccatagt cttttcttcc ggtgtgggat
    10321 taactcttat tccgcagctg caacgcgatt aagccggcaa caggcatgca gccagccgcg
    10381 ccgcaaaata caggccaaag ccatacaccg tgtgcgtcat gaggctcttg acgcggttaa
    10441 aattcggtgt cgctgttttg cccgaggcga tgcctgcgcc taggccgggc tgcagaataa
    10501 agtacggggc agcgaccgtt gccacaccga tcaccagcgc tggcaacaat gacggcgagc
    10561 gcagccaatc gaggccgtag acagccacga gcagggcggc aaagctgata ccgatagtgt
    10621 aatgcgcgca ccagcccaga agcagttcgt accgaaccgg tgtggattgc gagatatttt
    10681 cgtgtatcca ctgcccctgc ggcaggtggc cgatccagcg gccgagaaag gcatagttca
    10741 gcgatttgat gccaaatgcc ctgagaaaaa tcgtccaaag gtccatgaga aaggttgccc
    10801 cgagaccgat gaacaagacc cgcaagacgg gttcaaaagc gttattcatg ataaactcct
    10861 acagtttttc atatcatact gtaatttaca gtatactgta tttacttgca tcggcgatat
    10921 tttttgcagt tggtggagcg ctgacggtct tctgcaggca aaacctcatt ctcagcagct
    10981 ttgcctgcaa atttgggttt gcgcagccgc tcgctcacgc cagccgtctt tgcgcgggtt
    11041 atgcaaaaga aaggggtgcg aaaaaaacag gaataccggc atctgccggc gttcattttg
    11101 ctggtcttga cgcaggggcc cgcgcatggc ggtgccgtgc acgctgcgct cagcaaaaaa
    11161 ctgcccgcct acaacgccga caccggcgct gtctaccgca cgctgctcga aatggaacag
    11221 aacggcgaga tcgaattcac ctgggacact tcgtcatcgg gccccgcgcg caaaatctac
    11281 cagctcactc ctgcggggtg ggagaaactc gcgtactgga aaaaagatat cgaagggcgg
    11341 ctcgaatacc tgaatatatt tttgaagagc tacaagaaga tcaaaaaccc cggtaaaaaa
    11401 agctaactcc gcaagaaaag cagcatgaat acactgacgg caatacttaa agaagcacaa
    11461 aaatacgcac tctccattcg ccccaaagtc gggggatttc cggtgttagc agaggtgctg
    11521 cgacaggccg gagtgcatgt gaatcgctgg tctttacctt cctgccaatc catttacatc
    11581 atggatggcg gagcggtggt tcaacaagga acgcccttag tcagtggcac acatgaaatc
    11641 ccaaaattca atcgcgaagc cctaattacc gctctaagaa ctgaccagga tggccgcagt
    11701 acttttcctg aatttcttca ggcggtatgg aatgccggta tcatcgggta cgatgcggat
    11761 tttggaaggc gactggtgac atactacggt gcgacagggg aatcttacac tgaagagtac
    11821 ccggctgtgg aattgaaata gtaaagacca aacacaagac tcttgagaat gagcaccggc
    11881 agccaattat cgggaattca aatcgcgccc gattggcttc taatatatta cgtttccagc
    11941 gaagttctga ggctcgaagc taccgagacg cacgccgacc tgttcgacta gcgtgcgtat
    12001 gctgacaaga ggtcagcaga gaacgaccaa aagtcttgca gatttttcgc acagcgtgga
    12061 ttttacgcga ggttttggct ggccgtgtct tcacggcaag ccaaaatctg cccgaagcgc
    12121 ct
//
LOCUS       DAOQJF010000054        12062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2643_length_12062_cov_5.757308, whole genome shotgun sequence.
ACCESSION   DAOQJF010000054 DAOQJF010000000
VERSION     DAOQJF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2643_length_12062_cov_5.757308"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLF85_04545"
     CDS             complement(<1..700)
                     /locus_tag="PLF85_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE18959.1"
                     /translation="MARKVSLKQLRNIGIMAHIDAGKTTTTERILYYTGKSHKIGEVH
                     EGAATMDWMEQERERGITITSAATTCFWKDLNGEQIQINIIDTPGHVDFTVEVERSLR
                     VLDGAVTCYDGVAGVEPQTETVWRQADRYSVPRLCYVNKIDRMGADFYRAVSMMKDRL
                     GANAVPIQLPIGVESGFVGVIDLVEMEAVVWSGEELGAKFERKPIPEDMKEQAEEYHN
                     ALVSAVAEVNDTLTE"
     gene            complement(762..1235)
                     /gene="rpsG"
                     /locus_tag="PLF85_04550"
     CDS             complement(762..1235)
                     /gene="rpsG"
                     /locus_tag="PLF85_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNE18960.1"
                     /translation="MARRRTKSHTRNIRPDGIHGSVLVQKFINSMMLDGKKSTATQLF
                     YQALDRVQEKTGVAGLEQFLKAIENVKPQVEVKSRRVGGVTYQVPVEVYAARKQSLAI
                     RWLVGAARDRSGKTFSDKLANEIMDAINNNGGAIKKKDEVKRMAEANRAFSHYAW"
     gene            complement(1258..1632)
                     /gene="rpsL"
                     /locus_tag="PLF85_04555"
     CDS             complement(1258..1632)
                     /gene="rpsL"
                     /locus_tag="PLF85_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNE18961.1"
                     /translation="MPTINQLIRFGREKMVDKTKSPALKANPQKRGVCTRVMTATPKK
                     PNSALRKVARVRLVNGVEVTAYIPGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DTLGVEKRRKARSKYGTKRPKA"
     gene            complement(1719..6023)
                     /gene="rpoC"
                     /locus_tag="PLF85_04560"
     CDS             complement(1719..6023)
                     /gene="rpoC"
                     /locus_tag="PLF85_04560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNE18962.1"
                     /translation="MREFHDFNKIRIRLASPDKVREWSHGEVKKPETINYRTLKPERD
                     GLFCEKIFGTTKDWECYCGKFKSIRYKGVVCDKCGVEVTHSKVRRERMGHIELSYPIA
                     HIWYYRTVPSRIALVLDMSANDIKSVLYFERYVVIDPGESGLELKQLITQEEHGRLVE
                     LYGDKFYASIGADAVKELLAMVNIEEEIRIIRARIESKKKISDKRILKRLEILEAFRD
                     SNNRPEWMVLDTIPVLPPELRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLGLKA
                     PDIIVSNEKRMLQEAVDALFDNSRRKKSIKGKGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRTVIVVGPELKIYQCGLPKKMALELFKPFIMKWLVDKEYTQNIKSAKRMIEN
                     EEREVFEGLEEVIHEHPVLLNRAPTLHRLGIQAFLPVLVEGKAIQLHPLVCHAFNADF
                     DGDQMAIHVPLSPRAQLEAWLLILSAHNLLNPANGHPIVGPTQDMILGLYYLTADYAG
                     AKGEGFIYDNMDELHYALDRGIVQYRAKVRFAQGDKMFETTPGRLIFNQLLPANYPYV
                     NKPLTNKTVNEIIADLYKLKGAAVTVKFLDAIKRLGFQFATKFAPTISIDDVKIPESK
                     PKLIDKANKEVERVETEHRKGVITNEERYKKVIDIWTNTNEKITEDLFALLSQDQNGF
                     NPIYAMAVSGARGSRSQIRQLAGMRGLMAKPSGDIIELAIRSNFKEGLGVLEFYISTS
                     GARKGLADTALKTADAGYLTRRLVDVSQDVIVREDDCGTNEGFNMSADKEGEKVIVGL
                     RQKIAGRYLQQDIKDPNTDEVVYIRNTLITEEIAAQIETLGYETIKVRSILTCESVTG
                     VCGCCYGIDLATLQPVEKGEAVGIIAAQSIGQPGTQLTMRTFHIGGTATTKSAESAIK
                     APYDAVVLQIQGRQVNNRQKQRIMAKRGSVIIAQILARYTKNQFSEFSHEAGAKLIKG
                     EVLGKLKDKQGADAMLYSPAAGQIFIEKNDIFLLAPDYAIQLGAGSVLHIEEREVLKV
                     GQLLCEYDPYNALIIATHSGRLELVDVEDGKNLRIEETGDRTVGKLKKIVAYKNEKLV
                     PRAHIYEKGKLVAETPLPVGAILTIENGSAVQEGDIIAKIESKAEKTRDITGGLPRID
                     ELFEARHPKDPCHLAEIDGVLRDTGEIVRDKRVLRIIPEGLKGEEAEDNAVSISIPLH
                     KQIQVHDGEMVTRGEPLDDGVVDPHDLLRIMGEELTQRYLIREIQEVYRLQGVYINDK
                     HIEIIIRQMMRKVEVTDAGDTAFVPMSQVDRAIFKNENLRVEREGGAEASATPILMGL
                     TKASLNSESFLSAASFQETTRVLTDAAIKGKDDPLEGLKENIIIGHLIPAGTGMRDYR
                     DVRAYKSVMGDLFYTEEELEQLYHGTAEKPEEVAALAPPGRQVGEDEEEAEESDEE"
     gene            complement(6050..9790)
                     /gene="rpoB"
                     /locus_tag="PLF85_04565"
     CDS             complement(6050..9790)
                     /gene="rpoB"
                     /locus_tag="PLF85_04565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNE18963.1"
                     /translation="MSESQAVRVGEDTSSFKIVNFGKIRKSLDIPPLIEVQTISYNEF
                     LQVDAPTAKRKRVGLQSVFEDSFPIESSNGDVVLEFVEYTLGEPKRDIWDCKVNGLTY
                     AAALKATIRLIAIETGEVREQEVYMGDLPIMTNTGTFIINGAERVIVNQLHRSPGIFI
                     FFDEMKNIFSSRIIPDHKGSWLEFEMDAKGLLIARIDRRKKFPFTLLLKALGYGTNEQ
                     ILRLFYESESVVIKGVQSKTLAKFIGRRILTDVAHPETGEILIEAGQALNEDNLDILK
                     DAKIEKIEIIVNKGAVDHRVVFNSLDKDGVNSTEEALMEFIKNQKPLDYSPDHGDPEK
                     RAKNIERANAELHRLFFDAKTYNMGSVGRYKINARFKHLNPKEFGEEVDTQTLRPIDI
                     VETCKYMVNLIHEVPGYQYDDIDHLGNRRVRTVGELLTTQLKAGFTRMERVVKERMSM
                     NDVDIMTPQLLISIKPITAVMNEFFGASQLSQFMDQTNPLSEITHKRRLNALGPGGLT
                     RERAGFEVRDVHYTHYGRLCPIETPEGPNIGLITSMATYSRLNPYGFVETPYRVVEKG
                     KDTGKTIFLSANEEDHYVVAQANATLGENGEFLDKLVSCRYRGDFPLKSPEEIQLMDV
                     SPTQVISLSTSLIPFLEHDDANRALMGSNMQRQAVPLLNPDMPIVGTGMEFQIAYDSG
                     VCIIADYDGVVTKVDADGIEVERENGTKDKYRLVKFKRTNQSTTLNHQALVGCHNAPE
                     NGKITSITKSEMLLEAENGKVYSFPMQTFQGAMKPMVKEGAFVYRGDLLAGEIIKGRL
                     DQANKNVSRKATVLADGLATRYGRLALGKNITVAFMPWDGYNFEDAIILSEKLVKDDT
                     FTSIHIEEFEIQARETKLGREIITRDIPNISERAFRDLDDEGIIRIGAEVRAGDILVG
                     MVTPKGETDLTPEYRLLHSIFGEKAKEVRDSSLRVPNGHAGIVVDIVRFKRDEGDELP
                     AGIVEAVKVYVAQKRKISVGDKMAGRHGNKGVISTILSEADMPMLPDGTPIDIVLNPL
                     GVPSRMNLGQIFETQLAYIGKTLGINFETPVFDGAKWEDVQAYAEKAKLSPDSKIHLR
                     DGRSGEFFQQSAFVGTIYMLKLHHMVEDKMHARSTGPYSLVTQQPLGGKAQFGGQRLG
                     EMEVWALEAYGAAHTLQELLTVKSDDMQGRARVYESIVKGIHAIKPGVPESFNVLIRE
                     IRSLALDITILDSEEKPIEISDMDDEFSRIRRRIKVDSVENT"
     gene            complement(9886..10260)
                     /gene="rplL"
                     /locus_tag="PLF85_04570"
     CDS             complement(9886..10260)
                     /gene="rplL"
                     /locus_tag="PLF85_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNE18964.1"
                     /translation="MVQELLDKIGSLTIVQAAELVKAMEDKFGISAAAPVAVAAVAAP
                     AAGAAAAEEKTEFNVVLEGFSGDKKVDAIKKVREITNLPLMDAKKLVEGGTGVLKEGV
                     AKEEAEKIKKEIEALGGKIALK"
     gene            complement(10312..10839)
                     /gene="rplJ"
                     /locus_tag="PLF85_04575"
     CDS             complement(10312..10839)
                     /gene="rplJ"
                     /locus_tag="PLF85_04575"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNE18965.1"
                     /translation="MASAEKTELKDEYTSILKSNPNFIVTQYQGLDVAQISSLRKKLR
                     EAGGSYTVVKNNVFSLALKESGVAKDFPYDSTLKGPNAIAFASEDVPSVAKVLRDFSK
                     DNDKLKITAAVMESTYYDAKGVATIADLPSREQILAQLAAMLNSPATKIAGTLNNIMA
                     SMARGIKSVAEKNGQ"
     gene            complement(10852..11535)
                     /gene="rplA"
                     /locus_tag="PLF85_04580"
     CDS             complement(10852..11535)
                     /gene="rplA"
                     /locus_tag="PLF85_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNE18966.1"
                     /translation="MAKHGKKYRAAAEKVSSDKEYSLLDAMKILKEVTYTKFDSTLEG
                     HFNIRYKSSQNVRGIVQLPHGTGKKVKILVFAKGDKAEEARAAGADYVGDDDLIQKIQ
                     GGWVDFDFVVATPDMMKDVGKLGQILGKRGLMPKPKSGTVTTDMKGIIAQLTSGRIEY
                     RADKTGVVHVPMGRLSFNPDQLKDNAEAVFQAILKDKPSDAKGDYVVGMYVAGTMTPA
                     IKINIKELR"
     gene            complement(11538..11966)
                     /gene="rplK"
                     /locus_tag="PLF85_04585"
     CDS             complement(11538..11966)
                     /gene="rplK"
                     /locus_tag="PLF85_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNE18967.1"
                     /translation="MAAKKIVAQVKLQIPAGKANPAPPVGTALGPQGVNLMEFCKQFN
                     AKTQAQAGSIIPVVVTVFSDRSFTFITKVPPASDLIKKAAKIESGSKIPHKEKVGSLT
                     QAQLEAIAKQKMPDLNANDIEAAKQIIAGTARSMGVTIQG"
BASE COUNT         2637 a         3255 c         2973 g         3197 t
ORIGIN      
        1 tttctgtaag agtgtcattg acttctgcaa cggcagaaac cagagcattg tggtattcct
       61 ctgcttgctc tttcatatcc tcgggaattg gcttacgctc aaattttgca ccgagttctt
      121 cgcccgacca tacaacggct tccatctcaa caaggtcgat aacaccgaca aaacctgact
      181 ctacaccgat tggcaactgg atcggcacag cgttggcgcc caggcggtct ttcatcatcg
      241 acaccgcgcg gtagaagtca gcgcccatac ggtcgatctt gtttacatag cagaggcgtg
      301 gcacgctgta gcggtcagcc tggcgccaga cagtctcagt ctgtggctca acgcctgcaa
      361 caccatcata gcaggttacg gcaccgtcga gcacgcgcag cgagcgttct acttcaacag
      421 taaagtcaac gtgacccgga gtatcaatga tattgatctg gatctgctct ccgttcaggt
      481 ctttccaaaa gcaggtcgtc gcagcagaag taatcgtgat accgcgttcg cgctcctgtt
      541 ccatccagtc cattgtcgcg gcgccttcat gcacctcgcc aattttgtgg gattttcccg
      601 tatagtagag gatacgttct gtagtggtgg tcttgccggc gtcgatatgc gccataatac
      661 caatattgcg gagttgctta agacttactt tacgtgccat atttttcctt tatctgtaaa
      721 ctaaaaggga cgcggtaacc gcgtccctgt tcggagcctg attaccaggc gtaatgagag
      781 aatgcgcggt tagcctcagc catccgcttg acttcatctt tcttcttaat agcgccacca
      841 ttgttattga tggcatccat gatttcgttt gcgagcttgt cagaaaatgt tttgcctgag
      901 cgatcacgcg cagcaccaac aagccagcga atcgccagcg actgtttacg cgccgcgtaa
      961 acctcaacag gcacctggta agtaacgcca ccaacgcggc gtgattttac ttcgacctgc
     1021 ggcttaacat tttcaattgc tttaagaaat tgctcaagcc ctgctactcc cgttttctcc
     1081 tgaacgcggt caagcgcctg gtaaaaaagc tgggtagctg ttgatttctt gccgtcgagc
     1141 atcatcgagt tgatgaactt ctgcaccaat acactgccgt gaatgccgtc cgggcgaata
     1201 tttctggtat gacttttcgt tctgcgtcgt gccatgattc ctctttttaa ataagcctta
     1261 tgccttcgga cgcttggtcc cgtattttga gcgtgctttg cggcgctttt ccacaccgag
     1321 tgtatcgagt gaaccgcgca cgatgtggta acgaacaccc ggaaggtcct tcaccctgcc
     1381 gccgcgcacc agtactaccg agtgctcctg caggttatgg ccttcgccgg ggatatacgc
     1441 tgtcacttcg actccgttca ccagtcgaac cctggccact ttgcgcaagg cagagttggg
     1501 cttctttggg gtcgccgtca ttacgcgcgt acaaacaccg cgtttttgcg ggtttgcctt
     1561 aagcgctgga gatttggtct tatcgaccat tttttcccgg ccgaaccgga tcaactggtt
     1621 aattgttggc atagctatcc ttgaaatggc attttctatt catgagacat cgcgtcaacg
     1681 cagaagagga gtgagtgctg tgccccctcc ctctactgct attcctcatc gctctcctct
     1741 gcttcttctt cgtcctcacc gacctggcgt cccggagggg ccagcgcggc tacttcttcc
     1801 ggcttttctg ccgtaccatg gtacaactgc tccagctcct cttcggtata gaacaggtcg
     1861 cccataaccg atttgtatgc ccggacatcg cggtaatcgc gcatacccgt tccagcaggg
     1921 atcaggtgcc caataatgat attttctttc agaccttcga gcgggtcatc cttgcccttg
     1981 atcgcagcat cggtcagaac acgggtggtt tcctggaacg atgccgcaga gaggaaactt
     2041 tccgaattca gtgatgcctt ggtaagcccc atcaaaatcg gcgttgcaga tgcttcagcg
     2101 ccaccctcgc gctcgacgcg caggttctcg ttcttgaaga tcgcacgatc aacctggctc
     2161 atcggcacaa acgcggtatc tcccgcgtcg gtgacttcta ccttgcgcat catctggcgg
     2221 atgataatct cgatatgctt atcgttgata taaacacctt gcaggcgata cacttcctgg
     2281 atctcacgga tcaggtaacg ctgcgtgagc tcttcaccca taatccgcag caggtcgtgc
     2341 ggatcaacta caccatcatc gagcggctcg ccacgcgtga ccatttcacc gtcgtgaacc
     2401 tggatctgtt tatgcagcgg aatggagatc gaaaccgcat tgtcttcggc ttcttcaccc
     2461 ttcaggcctt ctggaataat ccggagtaca cgcttgtcgc ggacaatttc gcccgtgtcg
     2521 cgcagtacac cgtcgatctc tgccaggtgg cagggatctt tcggatgacg tgcttcgaac
     2581 agttcatcga tgcgtggcag accgccggtg atatcgcgtg ttttttctgc tttcgattcg
     2641 atcttggcga taatatcgcc ttcctgaacg gcgctgccgt tttcaatcgt gagaatcgca
     2701 cccacaggca gcggagtctc tgcgaccagc ttaccctttt catagatatg cgcccgagga
     2761 acgagttttt cgttcttgta ggcaacgatc tttttcagct taccgacagt acggtcgccg
     2821 gtttcttcga ttcgaaggtt tttgccgtct tcaacatcga ccagctcgag gcggccgctg
     2881 tgcgtcgcga tgatcagagc gttgtagggg tcatattcac agagcagctg ccctactttc
     2941 aggacctcac gctcttcgat atgcagcacc gaaccagcac caagctgaat cgcatagtca
     3001 ggcgccagga ggaagatatc gttcttttcg atgaatatct gtcccgctgc tggcgaatag
     3061 agcatcgcat ctgcaccctg cttgtctttc agctttccga gaacttcacc cttgatgagc
     3121 tttgcgcccg cttcgtggct gaattcagag aactggtttt ttgtatagcg tgctagaatc
     3181 tgcgcaatga tcacagaacc gcgtttagcc atgatccgct gtttctggcg gttattgacc
     3241 tggcgaccct ggatctgcag caccaccgca tcgtaaggag ccttgatagc gctctctgct
     3301 gatttcgttg tcgcagtgcc accgatgtgg aacgtacgca tcgtcagctg ggtacctggc
     3361 tggccgatcg actgagccgc aataatacct acggcttcgc ccttttctac cggctgcagc
     3421 gtggcaaggt cgatgccata gcagcaaccg cacaccccgg taaccgattc gcaggtcagg
     3481 atcgaacgta ctttaatggt ttcgtaaccg agcgtttcga tctgcgctgc aatttcttct
     3541 gtgatcagcg tattacgaat ataaacaact tcgtcagtgt ttggatcctt gatatcctgc
     3601 tgcaggtaac ggccggcaat cttctggcgc agaccgacga taactttttc gccctctttg
     3661 tctgcactca tgttaaagcc ttcgttcgta ccgcagtcgt cctcgcgaac gatcacgtcc
     3721 tgcgaaacgt cgacgagacg gcgcgtcagg tagccggcgt cggctgtttt caacgccgta
     3781 tccgcgagac ctttacgagc tcccgaagtt gaaatataga attcaagaac gccgagacct
     3841 tctttgaagt tcgaacggat cgcaagctcg ataatgtcac ccgatggttt tgccatcaga
     3901 ccgcgcatac cggcaagctg gcgaatctgc gaacgcgagc cccgtgcacc ggaaaccgcc
     3961 atcgcataaa tcgggttaaa accgttctgg tcctggctca gcagggcaaa aagatcttca
     4021 gtgatctttt cgttcgtatt cgtccagatg tcgatgacct tcttgtagcg ctcttcgtta
     4081 gttatcacac ccttgcggtg ctcggtttca acgcgctcga cctctttatt cgccttgtcg
     4141 atcagctttg gctttgactc aggaatcttg acgtcgtcaa tagagatggt gggtgcaaat
     4201 ttggtcgcaa actgaaagcc caggcgcttg atcgcatcca ggaatttcac ggtaaccgcc
     4261 gcacctttca gcttgtaaag atcagcgatg atttcgttca ccgttttgtt ggtcagcggc
     4321 ttattgacat atgggtagtt cgccggcagc aactgattga aaatcaagcg acccggggtc
     4381 gtttcgaaca tcttgtcgcc ctgcgcaaaa cgtactttcg cacggtactg cacaataccg
     4441 cggtcgaggg cgtagtgcag ttcgtccatg ttgtcataga tgaaaccctc gcccttcgcg
     4501 ccggcatagt cagcagtcag gtaatagaga ccgagaatca tatcctgcgt cgggccaacg
     4561 atcgggtgac cgtttgccgg gttgagcagg ttgtgcgcag aaagaatcag cagccaggct
     4621 tcgagctgtg cacgcggcga gagcggcacg tgaatcgcca tctggtcacc gtcgaagtca
     4681 gcattgaacg catggcagac cagcgggtgc agctggatag ctttaccttc taccagtact
     4741 ggcaagaacg cctggatacc gaggcggtgc agcgttggag cgcggttcag cagaaccggg
     4801 tgctcgtgaa ttacttcttc gagaccttcg aagacttcgc gctcttcatt ctcaatcatc
     4861 cgcttcgcgg atttaatatt ctgagtatac tctttgtcga caagccactt catgatgaag
     4921 ggcttgaaga gctcgagcgc catcttcttg ggaagaccac actggtagat cttgagttcg
     4981 ggaccgacaa cgattacagt acgacccgag tagtcgacac gctttccaag caggttctgg
     5041 cggaaacgcc cctgctttcc tttgagcata tcggagagtg acttaagggg acgattaccc
     5101 ttacccttga tcgatttctt gcgccggctg ttatcaaaaa gagcatccac tgcttcctgc
     5161 agcatgcgct tctcgttcga aacgatgata tccggagcct taaggcccag gaggcgtttc
     5221 agtcggttgt tacggttgat gacacgacgg taaagatcgt tgaggtcgga cgtagcgaaa
     5281 cgaccgcctt cgagctgcac catggggcgc agctccggtg gcagtaccgg aattgtatcc
     5341 agtaccatcc actcgggacg gttgtttgaa tcgcggaaag cttcgagaat ctcgagacgc
     5401 ttcaggatac gcttgtcaga aatcttcttc ttagactcaa tacgcgcgcg gataatacgg
     5461 atttcttctt cgatgttgac catcgcgaga agctctttaa ctgcatcagc tccgatagat
     5521 gcatagaatt tgtcgccgta gagctcaaca aggcgaccgt gctcttcctg ggtaatgagc
     5581 tgcttaagtt cgagacctga ctcgcccgga tcgataacca cgtagcgctc aaaatagagc
     5641 acgctcttga tgtcattggc agacatatcg agcacgagcg cgatgcggct cggcacagtc
     5701 cggtaatacc agatgtgcgc tatcgggtaa gagagttcga tatgtcccat gcgctcgcgg
     5761 cgcactttgg agtgagtaac ctcaacacca catttgtcgc agacgacgcc tttgtagcgg
     5821 attgacttga actttccgca gtagcattcc cagtccttgg tggtaccgaa aattttctcg
     5881 cagaaaagac cgtcacgctc tggtttgagc gtacggtagt tgatggtctc cggctttttt
     5941 acctcgccgt gtgaccattc ccgaactttg tcgggagaag cgaggcggat gcggatttta
     6001 ttgaagtcgt gaaattctct cattttttcc ccgtgctgac cttacaccct taggtgtttt
     6061 ccaccgaatc taccttgatg cggcggcgaa tgcgtgagaa ctcatcgtcc atatcagaga
     6121 tttcgatggg tttctcttcc gagtcgagaa tcgtaatatc gagcgcgaga gaacggattt
     6181 cacggataag aacgttaaag ctctccggaa ctcctggttt aatcgcgtgg atacccttaa
     6241 cgatagattc gtaaacgcgg gcgcggccct gcatgtcatc tgacttaaca gtcaggagct
     6301 cctgcagtgt gtgcgcggcg ccgtatgctt cgagcgccca gacttccatc tcaccgaggc
     6361 gctggccacc aaactgtgcc ttaccaccca atggctgctg tgtaacgagc gagtatgggc
     6421 ccgtcgaacg cgcatgcatc ttgtcttcaa ccatgtggtg aagcttgagc atgtagatcg
     6481 taccgacaaa tgccgactgc tggaagaact caccgctgcg cccgtcgcgc aggtgaattt
     6541 tagaatcagg gctcagcttt gctttttctg cataggcctg aacgtcttcc cactttgcac
     6601 cgtcgaagac cggggtttca aaattgatgc ccaatgtctt gccgatataa gcgagctgcg
     6661 tttcaaaaat ctggcccagg ttcatacgcg aagggacgcc aagcgggttc agcacaatgt
     6721 cgattggcgt tccatcaggc agcatcggca tatcggcttc gctcaggatt gtcgagatga
     6781 cacccttgtt accgtgacgg ccggccatct tgtcgccgac agatatctta cgcttttgcg
     6841 ctacatagac cttcacggct tctacgatac ccgccggcag ctcatcgcct tcatcgcgct
     6901 tgaagcgaac gatatcaacc acgataccag catggccgtt gggcacgcgt agcgatgaat
     6961 cccgtacttc ttttgctttt tcaccgaaga tcgaatgcag caggcggtat tccggtgtaa
     7021 ggtcggtctc ccctttcgga gttaccatac ctacaaggat gtccccagcg cgcacctcgg
     7081 cgccgatacg gataatacct tcgtcatcga gatcgcggaa tgcgcgctcg ctgatgttcg
     7141 gaatatcccg ggtaataatt tcgcggccga gtttggtttc acgcgcctgg atttcgaact
     7201 cttcaatgtg gatgctggtg aaagtatcat ctttcacgag cttctcagag agaataatcg
     7261 catcttcaaa gttgtatcca tcccatggca tgaaggccac agtgatgttc ttgccgagcg
     7321 ccaggcgtcc atagcgagtt gccagtccgt ctgccaggac agttgccttg cgcgatacgt
     7381 tcttatttgc ctggtcgagc cggcctttga tgatttcgcc ggcaagcaga tcgccgcggt
     7441 aaacaaatgc gccttctttg accatgggct tcattgcgcc ctggaaagtc tgcatcggga
     7501 agctgtaaac tttgccgttt tcggcttcga gcagcatttc gctcttggtg atcgaggtga
     7561 tcttaccatt ttccggtgcg ttgtggcagc ccaccagcgc ctggtggttc agcgtggtac
     7621 tctggtttgt gcgcttgaat ttaacgagac ggtatttgtc tttcgtaccg ttttcacgct
     7681 cgacttcaat accgtctgcg tcgaccttgg taaccacgcc gtcgtaatca gcaatgatac
     7741 agacaccaga gtcataggca atctggaact ccatgcctgt gccaacaatt ggcatatcag
     7801 gattcagcag aggtactgcc tggcgctgca tgtttgaacc catgagcgcg cggttcgcgt
     7861 catcgtgttc aaggaatgga atgagacttg ttgagagcga gataacctgg gtcggagaaa
     7921 catccatcag ctggatttct tctggcgact tcagagggaa atccccccgg taccggcagc
     7981 tcaccagctt atcgaggaat tcaccgtttt cgccgagcgt agcattcgcc tgcgccacga
     8041 catagtgatc ttcttcgttg gctgacaaaa agatggtttt gcctgtatct ttacccttct
     8101 caacaacgcg gtaaggtgtc tcaacgaagc cgtaaggatt gagccgcgaa tatgttgcca
     8161 tcgaagtgat aagaccgatg ttcggacctt ccggcgtctc aatcgggcac aggcggccgt
     8221 agtgcgtata gtgaacgtcg cgcacttcaa aaccagcgcg ctcgcgagta agaccacccg
     8281 gccctaaggc attgaggcgg cgcttgtgag ttatttctga aagcgggttc gtctggtcca
     8341 tgaactgtga gagctggctt gcaccaaaga actcgttcat taccgcagta atgggcttaa
     8401 tagagatcag gagttgcggc gtcatgatat cgacgtcgtt catgctcata cgctctttca
     8461 ccacacgctc catgcgtgtg aaaccagctt tgagctgtgt agtgagcagt tcgcccacag
     8521 tgcgcacgcg gcgattaccc aggtgatcga tatcatcgta ctggtaacca ggtacctcat
     8581 ggatcaggtt caccatgtat ttgcaggtct caacaatatc aatcgggcgc agcgtctgcg
     8641 tatctacttc ttcaccgaat tctttcgggt tgagatgctt gaagcgtgcg ttaatcttat
     8701 aacgccctac gctacccatg ttgtaggttt ttgcgtcaaa aaacaggcgg tgcagctcgg
     8761 catttgcgcg ttcaatattc ttcgcgcgct tctcagggtc accgtgatca ggactgtagt
     8821 cgagcggttt ctggttcttg atgaactcca tcagcgcttc ttcagtcgag tttacaccgt
     8881 ctttatcgag cgagttgaac accacgcggt ggtctactgc gcctttgtta acaataattt
     8941 caattttttc gatcttggcg tctttcagga tgtcaaggtt atcctcgttg agcgcttgcc
     9001 ctgcttcgat aagtatctca cccgtctcgg gatgagccac atcggtcagg atacgacggc
     9061 cgatgaactt ggccagggtc tttgactgga ctcccttaat gacaacggat tctgattcat
     9121 agaaaagtcg cagaatctgt tcgttggttc cgtagcctaa tgctttgagc agcagcgtaa
     9181 acggaaactt cttacgacgg tcgatacggg caatgagcag gccctttgcg tccatttcaa
     9241 actcgagcca tgagcccttg tggtctggaa taatacgtga cgagaaaatg ttcttcattt
     9301 catcgaagaa gatgaagata cctggcgaac ggtgcagctg gttcacaatc acccgctccg
     9361 ctccgttgat gatgaaagta cccgtgttag tcatgatggg caggtcgccc atatagactt
     9421 cctgttcacg aacttcaccg gtttcaatcg ctataagacg gatagttgcc ttaagggcag
     9481 cagcgtacgt gagcccgttt actttgcaat cccagatatc acgcttgggt tcaccaagag
     9541 tgtactccac gaattcgagc acaacgtcac cgtttgaact ctcgatcggg aaactgtctt
     9601 cgaataccga ttgcagaccg acgcgtttgc gctttgctgt gggagcgtct acctgcaaga
     9661 actcattgta cgagatcgtc tggacttcaa ttaacggagg aatatccagt gatttgcgaa
     9721 tttttccgaa attgacgatc ttgaaagacg aggtatcctc gccgactctt acagcttgcg
     9781 actctgacat tttgctcctc tggaaatcag cacaaaaaat ataaaagccc gccggttaaa
     9841 aaccggcaag gctaaaccag aaattggtag tagagaaaag aagcattact tgagcgcgat
     9901 ttttccgccc aaggcctcaa tttctttctt aattttttct gcttcttctt tagcaacacc
     9961 ctctttcaga acgcctgttc cgccctcaac gagcttcttt gcgtccatca gaggaaggtt
    10021 tgtgatttca cgaactttct tgattgcatc tactttctta tcgccgctga atccctcgag
    10081 aacaacgtta aattcagtct tctcttcagc cgcagcagca cctgccgcag gagcagcgac
    10141 tgctgcaacc gctacgggcg ccgcagctga aatgccgaat ttatcttcca ttgccttgac
    10201 gagttctgcc gcctgaacga ttgtcagcga cccaatttta tctaacagct cctgtaccat
    10261 actaactcct tctttcccct atgtgactct taagtatctc tactaagacg attattggcc
    10321 gttcttttcg gccacactct tgataccacg ggccattgat gccatgatat tgttgagcgt
    10381 accagcaatt ttggttgccg gtgaattgag cattgccgcc agttgggcga gaatttgctc
    10441 gcgcgaaggc aggtcggcta ttgtcgccac acctttcgca tcataatatg tcgactccat
    10501 gacagccgcg gtaatcttca gcttgtcatt gtccttactg aaatcgcgca gcacttttgc
    10561 cactgaggga acatcttcgc tggcaaaggc aatcgcgttg ggacccttca gcgttgaatc
    10621 gtatggaaaa tctttagcca cacctgattc tttcagggca aggctaaaaa cgttgttctt
    10681 gacaacagta tacgaaccac cagcctcacg cagcttcttg cgcaggctgc tgatctgtgc
    10741 tacatcgaga ccctggtatt gggtaacaat aaaatttgga ttagacttca ggatcgaggt
    10801 atactcgtct ttaagttctg ttttttccgc tgatgccatg aaaagccccg cttaacgcaa
    10861 ttccttaata ttgatcttga tcgcaggagt catagtacct gccacataca taccaacgac
    10921 gtagtcgcct tttgcgtctg acggcttgtc tttcaaaata gcctggaaca ctgcttctgc
    10981 attgtctttc agctggtcag gattgaatga caggcgcccc atcggcacat gcactactcc
    11041 tgttttgtcg gcacggtatt caatacgacc cgaggtcagc tgggcaatga tacccttcat
    11101 atcggtcgtc actgtgcctg atttcggttt aggcatcaga ccacgctttc cgagaatctg
    11161 gccaagttta ccgacatctt tcatcatgtc cggtgtcgca accacgaaat cgaaatcaac
    11221 ccagccgccc tggattttct ggatcaggtc gtcgtcgcca acataatctg caccggctgc
    11281 acgcgcttct tcagctttat caccctttgc aaatacgaga atcttgactt tcttaccagt
    11341 gccgtgtggc aactgcacaa taccccgcac attctgcgag cttttgtaac ggatattgaa
    11401 gtgcccttcg agcgttgaat caaacttcgt atacgtaact tctttcagta tcttcattgc
    11461 atcgagcaat gagtattctt tgtcagagct tactttctct gccgcagcgc ggtatttctt
    11521 accatgcttt gccataatta cccctggatg gtaacgccca tagaccgggc ggtacctgcg
    11581 atgatctgct ttgccgcctc aatgtcgttg gcattaaggt ctggcatctt ttgtttggcg
    11641 atagcttcca gctgcgcctg ggtgagcgag cccaccttct ctttatgagg aatctttgat
    11701 ccgctttcaa tcttagcagc ctttttaatc aggtcactgg caggcggaac ctttgtaatg
    11761 aaggtaaatg aacggtctga aaaaaccgtg actacaacgg gaatgatact ccctgcctgc
    11821 gcctgcgtct tcgcgttaaa ctgcttacag aactccatca ggttcacacc ctgaggacca
    11881 agcgcggtac ccactggagg tgccgggttt gctttgcccg ccggaatctg gagtttaacc
    11941 tgggctacta ttttttttgc tgccatgaat accctttatt tacttgcgag ctgccatttt
    12001 gcaaaattgt gacataccgc aaactatatt ttacttacct gcaggtagtc gagttcgaca
    12061 gg
//
LOCUS       DAOQJF010000055        12062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2644_length_12062_cov_5.104023, whole genome shotgun sequence.
ACCESSION   DAOQJF010000055 DAOQJF010000000
VERSION     DAOQJF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2644_length_12062_cov_5.104023"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLF85_04590"
     CDS             <1..848
                     /locus_tag="PLF85_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNE18968.1"
                     /translation="SEWRYRNPLASGDSLVVGISQSGETADTLAGLREAKAKFMRVLS
                     FLNNVNSTMARESDAMINLMAGPEIGVASTKAFTAQVLNLLFFSLYVGSIKWLIPAEE
                     RRELLDEMRALPEKMRRILQNHEKIKEIAEFLKDKNDTIFLGRTYNYPSALEGALKLK
                     EISYIHASGYAGGEFKHGPLALVSDNVPVVIIAPQGEIRTKMISNLKEAQSRKGKIIS
                     IITEGDEEIKKESDFYFEIPACTESLSPLLVAIPLQLLAYYTAIARGCDVDRPRNLAK
                     SVTVE"
     gene            850..1977
                     /locus_tag="PLF85_04595"
     CDS             850..1977
                     /locus_tag="PLF85_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE18969.1"
                     /translation="MPGKPKAKKAAKKSSKAVKPAKAKKTVTPEKQQGGPVPVVPAPL
                     PVAARSAAVDAAAEARSERDERIGQLLPDIRKQNPDALAAFFEDFSDDIYNFPMRMFR
                     FNEDEASEFYLYAFEHLRDGRKIASFQGKAKFTTWFYAVLRNLTIDFLRSQKDKMRFT
                     TYLKADASGKMVDAIESASDPRDQNTFEETLFEQFNASLVSLALPQRVLFKLAYAWYF
                     ELDSTEIDYLCDLHKASAAQILPRLQELKKVAHEKSVEVRDLEEKLTANFQGISVLET
                     RIENFFRENPDIDKRRESWSENFFSTAVPPQVIDMIQSLMKKKKKQQSLLLHQKKSLL
                     SIRVPYKDLGDLLQSTQGVLSVQLIRIVEKLSQSLTNQPAE"
     gene            complement(1979..2842)
                     /locus_tag="PLF85_04600"
     CDS             complement(1979..2842)
                     /locus_tag="PLF85_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18970.1"
                     /translation="MDNPHQRAQELIREFVEFNERYAAQIPLAIPKGTLKTIDHYRDT
                     YFRYLKDSEYKSNICYMLQLIEYQLWQYRLFKPQYSLESALFFQLLVTQGIIIEAVVF
                     ALIADPLVIPDSTDRSKGGSDPQHENLLRMVRKRTFANHIQQLRTMQVLSDELCDRLD
                     QFRNEIRNLVHLQNWEGRLYRQIPQAEYSKHLQEFDDLLRQLNTAITGTPDLPGLLAY
                     YQLDGRRLRGTVKYFNRNSMRGQIEAKGPHDYIPFFHDASAPQLRKGDNVSFVLEVAA
                     QGIRATEVTLE"
     gene            complement(2863..3930)
                     /gene="gcvT"
                     /locus_tag="PLF85_04605"
     CDS             complement(2863..3930)
                     /gene="gcvT"
                     /locus_tag="PLF85_04605"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNE18971.1"
                     /translation="MKSTPLLEEHRKLSAKFTEFAGYNMPVSYGAVKDEVMAVRERCG
                     MFDVSHMLPIFLKAGTRDALVAALNRVCVRPTDKLKPGKAQYNALYNEKAGLVDDITM
                     SAISDTHWTIVANAGNRDAVVAHLQAHCAGISIEPWSDYVLLAIQGPKAADFIAKRWP
                     LAEQLYYYEVALPEPKLFIGRMGYTGEDGFEIACDAQAGIKLWQELHSSGVAACGLAA
                     RDVLRLEALMPLYGHELSTELRPQESGIPFIYADHDCIAPRELREKPVENITCGFHMQ
                     TEGVPRQGYAIADATGATVGEVTSGTFSFTKNYGMGMMRISVQAKANPLSVVIRGEHK
                     PITVLDKAPIAGSVKRRPKTK"
     gene            complement(3978..6230)
                     /locus_tag="PLF85_04610"
     CDS             complement(3978..6230)
                     /locus_tag="PLF85_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNE18972.1"
                     /translation="MLIAMDDWKNTILQNLNEPQQQAVQTTRGPLLILAGAGSGKTRT
                     IIHRMAWLIHVEKVPAWQIAAVTFTNKAAEEMRTRLLNTAGPIASESTVRTFHSLGLF
                     LLRRNAQFLNYPENFSIWDDTDQQQALSGILEKFDGKYTKVQYRYFANTISSFKDKLI
                     LPENLAEQVDLDQYEFGDILPEVYQLYEVKKSASLAVDFADLISLPCHLFEKHPELLE
                     RWQNRYPYWLIDEYQDTNYAQYRFVQHVAARDRNLCVVGDDDQAIYGWRGADVKNILD
                     FSRDFKDATIIKLEENYRSTKVILDIANAVIENNFERMPKRLFTERQGGSAPVLCTTG
                     DDVQEAAKVVGLVREALRENPPSEVAVLYRTNSQSRMLEEAMLNAKIPYRVYGGLSFF
                     ARKEIKDTLAYFRMIVNGKDEAAFARVINSPPRGIGEKSVEKIFGARELHGQADFVAL
                     LLDESMNPLSGKGREAAVKFARDINAFRKSVAAGTDLGFFLDELLDKTGLAKAWEEED
                     RLLGTGRMEYIGELRNSLLNYQRQSENAGLGDYLQQISLITTSAPDEEPGSEGAVSLM
                     TVHNAKGLEFGTVVIAGFEKDLFPHFLAERDGDISEERRLFYVAVTRAKNKLFFTQAE
                     RRMRQGFYESSRISPFWNEIPEGMVTRERSQTFAPSGSAVRQSYIPRAPAAAKPAPKN
                     PGFGAAIAPQARTAGNGYATGDKVSHSNFGRGRVLKIEGDGDSARIHIFFDDGKTRKF
                     LLKFTELKKL"
     gene            6322..6543
                     /locus_tag="PLF85_04615"
     CDS             6322..6543
                     /locus_tag="PLF85_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNE18973.1"
                     /translation="MKATQLRANLYKTLDEVIATGKPVRVERKGKVVLLVPEQDVPKR
                     RKFIKRKLLLVPDEEIIHMDWSKEWKYEK"
     gene            6540..6914
                     /locus_tag="PLF85_04620"
     CDS             6540..6914
                     /locus_tag="PLF85_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE18974.1"
                     /translation="MIYLDTHCVVWLYEGKEHRFSQRVLKLIQTEDLWVSPMVALELE
                     YLYESRKIGQRSVEVLSKLKKNFGLQVDSVDFAQAVELACDESWTREPFDRVITAHAR
                     ASESILVTYDHEIAAHYRHAVC"
     gene            complement(6926..7777)
                     /locus_tag="PLF85_04625"
     CDS             complement(6926..7777)
                     /locus_tag="PLF85_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HNE18975.1"
                     /translation="MRNLLRLAKASIIFTVVVTGFTGMLMARRSLPTLWEGFWILVSL
                     TLSSGSSAIMNNLLDYDMDKQMRRTQWRSEIIDQYGKSKLWFLAMMGSLISFAPLVYY
                     GRLIAVLLTAGAIASYAIWYTLYLKRRGPFGAIVGGLPGALPVLIGAYGLSDRFAPDV
                     WLLFAFMMLWQPAHFWALTLKIQGEYEKGGVPVLPLVFGDHYTRLYILIYGMSLPPLA
                     LAVGLSAGYSLVYLIGSGLASLYYVVRTVRGIYREEHYGRAFFASIIYMLVVMVLLNV
                     DIFLQKL"
     gene            complement(7846..8781)
                     /locus_tag="PLF85_04630"
     CDS             complement(7846..8781)
                     /locus_tag="PLF85_04630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE18976.1"
                     /translation="MPVAENTASHLPLVLIVDDSETAILYLRQLLQGEFKVVTASNLI
                     EFWAQLAGEPPDLILMDVVQKEISGFELVRQIKRTDEYKGIPVIFVTRLDSAADIEAG
                     FEAGCHDYVTKPVMQRELKARMRAALRLKNLEQELRMRSITDYLTGAYNRRYFYEVMA
                     GNLSYAQRMKRNLCIAILDIDFFKKVNDRYGHEAGDAVLQHFTHTIRSQIRKYDVLAR
                     FGGEEFVIQFFDCDLQKSTELLERVQHRLLESPCDIPDGHVSYTFSAGIACLHESESA
                     DPIDALVEMADRRLYEAKTSGRNRIITIDKPKVLQ"
     gene            8947..10137
                     /locus_tag="PLF85_04635"
     CDS             8947..10137
                     /locus_tag="PLF85_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE18977.1"
                     /translation="MPHGSRRIFSPGETIFSEGERQDIAFIIEQGEVEIWTTIEDEKR
                     TLNILKAGALFGELALVDRQPRSASATALGETVLTVVTQQQVDERLADADPILRMVLF
                     VVMRHFRSELQRARGHYGPYDRNRETPAPVENAQKRIDEAIELIKIEAELKSAMQNEE
                     FRLMYQPIHELKTQKIAGLEALIRWKSPTRGEIMPSAFMPLAESTSLIVPIGNWVIDA
                     GLADYLQFREAAKADITLSFNLARRQMEHGGFLPYLTERVDRHGIAPHAIKLEMLERN
                     LFESDTVTGWIQDCVARGFSILLDDFGTGYSSLQYLQEYQPAALKIDRSFVKGLGQRV
                     ESDRICRAIIELAHALGIGVIAEGVETLQQARILTDMGCQQAQGYFFSPPLSADAIRQ
                     RLSA"
     gene            complement(10148..10822)
                     /locus_tag="PLF85_04640"
     CDS             complement(10148..10822)
                     /locus_tag="PLF85_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18978.1"
                     /translation="MRKITLLLLACFAVTATGAKKPKTTIKEYFFLLPADALLMGMES
                     VDVRKKVLARNTDGLGLMFEVEEVIDDAANGYLAIKYSADPAPGTLALAVWRTDKGED
                     IIGVSNGDGSPTFFTQQNGNWKNVTANVFGDFQKSLFKPKAKWPKLCGAEDTSSSPQQ
                     MEFPRGFDCPVPRKGLNITCVYSPKCMVYGSVSVFETDFKKKPTDYYAVTKKQFIWNK
                     DRFIAK"
     gene            complement(10837..11490)
                     /locus_tag="PLF85_04645"
     CDS             complement(10837..11490)
                     /locus_tag="PLF85_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18979.1"
                     /translation="MWTKRIILFLLVVSLAYAVGSKSTISSYFWTFPDEVMPDGLKEF
                     EDRTAALRRKAGSDETIGLIVQDDKNGYMALGFKTRQQSARHEFAMWRHPGDKPVVGV
                     NSLYSGVGGIRSTLYFITLDEAYPGIRWKIITPEVFADFNRATFQPKANAPAGCKDEP
                     PLAEFPENFSCKLPQKGLTVVCQFNLNCDTPTKLTPKDFYAKPVVKFEWKKDRFVAK"
     gene            complement(11562..>12062)
                     /locus_tag="PLF85_04650"
     CDS             complement(11562..>12062)
                     /locus_tag="PLF85_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNE18980.1"
                     /translation="SSEYNTRRAECEAGIAVLRKKYAAVQSLRDANLVLLATVRGELD
                     ASTYSRCEYVIEENDRVVEAVDQLKQADAAAFGRLMYASHEGLSRKYAVSCTELDLLV
                     AIAKDCGVTGARMMGGGFGGCTINLVEESGLPGFISAAESRFAAQFGHAPLVYEVQIS
                     QGARGL"
BASE COUNT         2712 a         3259 c         3287 g         2804 t
ORIGIN      
        1 cgtctgaatg gcgctaccgt aacccgctcg cgagtggcga ttcgctcgtc gtgggtatca
       61 gccagtcagg agagaccgcg gatacgctgg cgggcctgcg tgaagccaaa gcgaaattca
      121 tgcgagtcct ttcctttttg aacaatgtca actcgaccat ggcgcgcgaa tcagacgcga
      181 tgatcaacct gatggcaggt cccgagatcg gcgtggcttc aaccaaggcc tttaccgcgc
      241 aagtgctcaa cctgcttttc ttctcgctct acgtgggttc gatcaagtgg ctgattcccg
      301 ccgaagagcg gcgcgagctg ctcgatgaaa tgcgtgcgtt gcccgaaaag atgcggcgca
      361 ttttacagaa ccatgaaaag atcaaagaga tcgctgaatt tctcaaggac aagaatgata
      421 ccatcttcct cgggcgcacc tataactacc cgtcagcgct cgagggtgcg ctcaagctca
      481 aagaaatctc gtatatacac gcctctgggt atgccggtgg cgaattcaag catggtccgc
      541 tcgcgctggt gagcgataac gtaccggtgg tcattattgc gccacaaggg gaaatccgca
      601 ccaagatgat ctctaacctc aaagaggcgc agtctcgtaa gggtaaaatc atttcgatta
      661 ttaccgaggg cgacgaagag atcaagaaag aatcggattt ctatttcgag atacctgcct
      721 gcaccgagag cctttctcca cttttggttg ccattccact gcagcttctg gcatactaca
      781 cggcgattgc gcgcggttgc gatgtcgaca gaccccgtaa cctggcgaaa tcggtaaccg
      841 tggagtagaa tgccaggtaa gcccaaagca aagaaggcag caaaaaaatc aagcaaggcg
      901 gtcaaacccg caaaagcgaa gaagactgta accccggaaa agcaacaggg tggtcctgtg
      961 cccgtcgtgc ctgctccttt gccggtggcg gcgcgctccg cagctgtaga tgcagccgct
     1021 gaagcgcgtt ctgagcgcga tgagcgtatc gggcaactat tgcctgacat caggaagcag
     1081 aaccccgacg cgcttgcagc gttcttcgaa gatttttctg acgacattta taacttcccc
     1141 atgcggatgt ttcgcttcaa cgaagatgaa gcgagcgaat tctatcttta tgcctttgag
     1201 cacctgcgtg atggcagaaa gatcgccagc ttccagggca aggcaaaatt cactacctgg
     1261 ttctatgcgg tgctgcgcaa cctcacgata gactttctgc gttcgcagaa agataagatg
     1321 cgcttcacta cttacctgaa agctgatgcc tcgggcaaaa tggtcgatgc gatcgaatct
     1381 gcgtctgacc cccgcgacca aaatacgttc gaagaaacct tgtttgaaca gtttaacgca
     1441 tccctggtct cgctggcttt gccgcaacgg gtattattta agctggcgta tgcgtggtat
     1501 ttcgaactcg acagtaccga gatcgactac ctctgcgatc tgcacaaggc aagcgctgcc
     1561 cagattctgc cccggttgca ggagctaaaa aaagttgcgc acgaaaagtc cgtcgaggtg
     1621 cgcgatctcg aagagaaact cacggcaaac ttccagggca tctcggttct ggaaacacgc
     1681 atcgagaact tcttccgcga gaatccagac atcgacaagc gccgcgaatc gtggagcgag
     1741 aattttttct cgaccgcagt gccgccccag gtgatcgaca tgatccagag cctgatgaaa
     1801 aaaaagaaaa agcagcagtc gctgctgcta caccaaaaga agagcctgct ttccatacgc
     1861 gtgccgtata aagacctcgg ggacctgctg cagagtacgc agggagtgct ctctgttcag
     1921 ctgatccgca ttgttgagaa gctgagccag tcgctcacca atcaaccggc tgaataagtc
     1981 actctaacgt tacttccgtt gcccgaatgc cctgcgcagc gacctcgagc acaaacgaaa
     2041 cattgtcgcc cttgcgcaat tgtggggccg aagcatcgtg aaaaaagggg atataatcat
     2101 gcggaccttt cgcttctatc tgtccccgca ttgaattacg gttaaagtat tttaccgtac
     2161 cccgcaggcg ccggccgtca agctggtaat aggcgagtaa tcctggcagg tcaggcgtgc
     2221 ctgtgatggc agtattgagc tgccgcagca ggtcgtcaaa ctcctgcagg tgcttcgagt
     2281 attctgcctg cggtatctgc cggtagagtc tgccttccca gttctgcagg tgaaccaggt
     2341 tgcgtatttc attacgaaac tggtcgagcc tgtcgcacag ttcatcgctc agaacctgca
     2401 ttgtgcgcaa ctgctgtatg tggttggcaa acgtgcgctt gcggaccatg cgcagcaggt
     2461 tttcgtgctg cggatcgctg ccgccctttg acctgtcagt tgaatcggga ataacgagcg
     2521 gatcggcgat cagggcaaaa acaacggctt cgataatgat tccctgtgtt accagcagct
     2581 ggaagaatag tgcactttcg agcgagtact gcggcttgaa gaggcggtac tgccacagct
     2641 ggtactcgat cagctgcagc atgtagcaga tgtttgattt atactcggaa tctttcaggt
     2701 aacgaaagta cgtgtcgcgg taatggtcga tcgtcttcag cgtgcctttg ggtatcgcca
     2761 gcggaatctg cgccgcgtat ctttcgttga actcgacaaa ctcgcggatc agctcctgtg
     2821 cgcgctggtg tgggttatcc atgccaatcg gccttttagt gattatttgg tcttcggacg
     2881 cctttttaca ctccccgcga tcggcgcttt gtcgagcacg gtaatcggtt tgtgttcgcc
     2941 gcgaattacg accgaaagcg gattggcctt cgcttgtacg gaaatccgca tcatgcccat
     3001 gccgtagttt ttcgtgaacg agaatgtacc gcttgtaacc tcaccgaccg tcgcaccggt
     3061 tgcatcggct attgcataac cctgccgcgg tactccttcg gtctgcatat gaaacccgca
     3121 ggtaatattt tcgacgggct tctcgcgcag ctcgcgtggg gcaatgcagt catggtcggc
     3181 atagatgaac ggaatgcctg attcctgcgg gcgcagttcg gtagagagct catggccata
     3241 gagcggcatc aatgcctcaa gccgcagtac atctcgagcc gcaagaccac aggcagcaac
     3301 ccctgacgag tgcagctcct gccagagttt aatacctgcc tgcgcatcgc aggcgatttc
     3361 aaagccgtct tcaccggtat agcccatgcg gccgataaac aacttcggct cgggcagcgc
     3421 tacctcgtag taatagagct gctcggccag cggccagcgt ttagctataa aatctgcggc
     3481 ctttggcccc tgtatggcga gcagaacgta gtcgctccat ggttctatgc tgatgccagc
     3541 gcagtgcgcc tgcagatgag ccacaactgc gtcgcggttg ccagcattgg cgacaatggt
     3601 ccagtgtgta tcgctgattg ccgacatcgt gatgtcgtcg acaagacctg ccttttcatt
     3661 gtagagcgca ttgtactgag ccttaccggg cttgagtttg tcagtcggcc tgacgcatac
     3721 cctattcaac gcagcaacca gcgcgtcgcg ggtgcctgcc ttaaggaaaa tcggcagcat
     3781 gtgtgaaaca tcaaacatac cgcagcgttc gcgaactgcc atcacctcat ctttgaccgc
     3841 gccgtatgag accggcatat tgtagccggc aaattcggtg aatttggcgc tgagcttgcg
     3901 gtgttcttcg agcaagggag tggatttcat gcgatactgt agagacgcgc cctgccgcgt
     3961 ctccattttt acaggcgtta gagcttcttc agttcagtaa atttcagcag gaacttgcgg
     4021 gtcttgccgt cgtcgaagaa aatatggatg cgtgccgagt caccatcgcc ttcaatcttg
     4081 agcactcggc cgcgcccgaa attggagtgg ctcactttgt cacccgtggc gtacccgtta
     4141 ccggctgtac gagcctgcgg agcaatcgct gcgccgaagc ccgggttctt tggtgcaggt
     4201 ttcgccgccg ccggggcgcg tggaatatat gactgccgca cagcactgcc cgatggtgcg
     4261 aatgtctggc tgcgttcgcg cgttaccatg ccctcaggaa tctcgttcca gaaaggcgaa
     4321 atgcgcgagc tctcatagaa accctgccgc atgcggcgct cagcctgcgt gaagaagagt
     4381 ttattctttg cgcgcgtcac agcaacatag aacaaccgcc gctcttcgct gatatcgccg
     4441 tcgcgctcag ccagaaaatg cggaaacaaa tctttctcaa agcccgcgat gaccacggtg
     4501 ccgaattcaa gacccttcgc gttgtgtacc gtcatgagac tcacggcgcc ctcgctgccc
     4561 ggctcttcgt cgggtgcgct ggtggtgatc agcgaaatct gctgcaggta atcgccgagg
     4621 ccggcatttt cgctctgccg ttggtagttc agaagcgagt tgcgcaattc gccgatgtat
     4681 tccatacggc cggtgccgag cagacggtct tcttcttccc aggctttcgc gagtcctgtc
     4741 ttgtcgagca gctcatcgag aaagaagccc aggtcggtac cagcagccac cgacttgcga
     4801 aaagcattga tgtcgcgggc aaacttcacc gctgcctcgc gtcccttgcc cgaaagcggg
     4861 ttcatcgact cgtcgagcag cagtgcgaca aaatctgcct ggccatgcag ttccctggcg
     4921 ccaaaaatct tttcgaccga tttttcaccg atgccgcgcg gcggcgagtt gatcacgcgc
     4981 gcaaatgccg cttcgtcctt gccattgacg atcatacgaa agtaggcgag agtatctttg
     5041 atctctttgc gggcaaagaa cgacaggccg ccatagaccc ggtacggtat ctttgcattg
     5101 agcatcgcct cttcaagcat gcgcgattgg gagttcgtgc ggtacaggac tgcaacctca
     5161 gaaggcgggt tttcccgcag tgcttcgcgc acgaggccta ccaccttcgc agcttcctgc
     5221 acatcgtcgc ccgtagtaca gagtaccggc gctgagccac cctggcgttc ggtgaaaagc
     5281 cgtttgggca tgcgttcaaa gttgttctct atgaccgcgt tggcaatatc gagaatcacc
     5341 tttgtcgaac ggtagttttc ttcgagcttg atgatcgtgg catctttgaa gtcgcgcgaa
     5401 aaatcgagta tatttttgac atccgccccg cgccagccat agatcgcctg gtcgtcatcg
     5461 cctaccacgc acaggttgcg gtcgcgcgca gcgacgtgct gcacgaatcg gtactgcgca
     5521 tagtttgtgt cctggtactc gtcgattagc cagtaggggt aacggttttg ccagcgctcg
     5581 agcagttcgg ggtgtttctc aaacaggtgg cagggcagag agatgaggtc ggcaaagtct
     5641 acagccagcg aggcggattt tttcacttca tagagctggt agacttcggg caaaatatcg
     5701 ccgaattcgt actggtcgag gtcgacctgc tcggccagat tctcaggtag aatcagcttg
     5761 tctttgaaag aagagatcgt gttggcaaaa taccggtatt gcaccttggt gtatttacca
     5821 tcgaatttct cgagaatgcc tgatagcgcc tgttgctggt cggtgtcgtc ccagatcgag
     5881 aagttctccg ggtaattgag aaattgcgcg ttgcggcgta gcaggaaaag tcccagcgag
     5941 tggaacgtgc gtaccgtgct ttccgatgcg atcgggccgg ccgtattcaa caggcgcgtg
     6001 cgcatctctt cggctgcctt gttcgtgaac gttactgcgg cgatctgcca ggccggtacc
     6061 ttctcgacat gtatcagcca cgccatgcgg tgtataatcg tgcgcgtttt gcccgagccc
     6121 gctccagcca aaatcagcag cggcccgcgc gtcgtctgca cggcctgctg ctgcggctcg
     6181 ttcagattct gcagaatggt atttttccag tcatccatgg caatgagcat cccggggcgg
     6241 ccctcgtgtg agtcgaccgc gtatgcgtct aattacgtac gtttttcttg acaaccgtac
     6301 gtaaaggtgt atttgagggt tatgaaggcg acacaacttc gggcaaatct ctataagact
     6361 ctcgatgagg tgattgccac tggcaagccc gtcagggttg agcgtaaggg caaggtggtt
     6421 ttgctggttc cggagcaaga cgtaccgaag cgtcgcaagt ttatcaagcg caaacttttg
     6481 ttggttccag atgaagaaat aattcacatg gactggtcaa aagagtggaa gtatgagaaa
     6541 tgatctatct cgatacacat tgtgtcgtct ggctttacga gggaaaggag catcggtttt
     6601 cgcagcgggt cttgaaattg attcagactg aagacctgtg ggtctcgccc atggttgcgc
     6661 tcgagcttga atacctctac gaaagccgaa agattggcca gcgctcagtc gaggtcttaa
     6721 gcaagctgaa gaaaaacttt ggtctgcaag tcgattcggt tgattttgca caggcagtcg
     6781 aacttgcatg tgatgaaagc tggacacgtg aaccttttga ccgcgttatt accgctcatg
     6841 cacgcgccag tgaatcaatc ttggtgacct acgaccatga aattgccgct cactaccgcc
     6901 acgcagtgtg ctagcctgat tctcgtcaaa gcttttgaag aaagatatcc acgttcagca
     6961 gcaccatcac gaccagcatg taaatgatcg aggcgaagaa cgccctgcca taatgctctt
     7021 ctctgtaaat accgcgcacg gtgcgcacga cgtagtagag cgaggcgagc ccgctgccaa
     7081 tcaggtacac cagactgtaa ccggcagata atcccaccgc gagcgcgagc ggtggcagcg
     7141 acataccgta gatgagtatg tagagccggg tgtagtggtc gccaaaaacc agtggcagca
     7201 caggaactcc gcctttttca tattcaccct ggatcttgag ggtcagcgcc cagaagtgtg
     7261 cgggctgcca cagcatcata aaagcgaaga gtaaccagac gtcaggggca aaacggtcgc
     7321 tgagcccata ggcgccgatg agtacgggca gcgcgccggg caggccgccg acgattgcgc
     7381 cgaatgggcc gcggcgtttc aggtacagcg tgtaccagat ggcataagag gctatagctc
     7441 ccgctgtcag cagcacggcg attaaccggc cgtaatagac caaaggagca aatgaaatta
     7501 atgagcccat catcgcgaga aaccagagtt tcgatttgcc gtactggtcg atgatctctg
     7561 agcgccactg ggtgcggcgc atctgcttat ccatgtcgta gtcgagcaga ttgttcatga
     7621 ttgccgatga acccgaagac agcgtcagcg aaaccagtat ccaaaatccc tcccacaggg
     7681 tgggcagcga gcggcgcgcc atcagcatac cggtaaaacc ggtcaccacg acagtgaaga
     7741 taattgaggc cttcgccaac cgcagcagat tgcgcatgca gtagctctct gcacgcgaga
     7801 ctcgctgcaa agccaaacta aaggggattg cctgccgaga cgagtctact gcagtacttt
     7861 gggcttgtcg atggttatga tgcggttacg ccccgaagtt ttggcctcat agaggcgccg
     7921 gtcagccatt tcaaccagcg catcaatggg gtcggcactt tcgctttcgt gcaggcaggc
     7981 gatccctgcg ctgaacgtat agctgacatg gccatcgggt atgtcgcagg gtgattcgag
     8041 caggcggtgt tgcacgcgtt caagcagttc ggtcgatttt tgcaggtcgc agtcaaaaaa
     8101 ctggataacg aattcttcgc cgccaaacct ggcaagtacg tcatacttgc gtatctggct
     8161 gcggatcgta tgcgtaaaat gctgtagcac cgcgtcaccg gcttcgtgac cgtaccggtc
     8221 attcaccttc ttgaaaaagt caatatcgag aatcgcaatg cagaggttgc gcttcatgcg
     8281 ctgtgcataa ctcaggttgc cggccattac ttcgtagaag taccgccggt tataagctcc
     8341 cgtcaggtag tcggtgatcg agcgcatacg cagctcttgc tcaaggttct tcagccgcag
     8401 cgccgcgcgc atacgggcct tgagttcacg ctgcatgacc ggcttggtta catagtcgtg
     8461 gcagcctgcc tcgaagccgg cctcgatatc tgcagcgctg tcgagccgtg tgacaaaaat
     8521 cacgggaata cctttgtatt cgtcggtgcg tttgatctgc ctgacgagtt caaaacccga
     8581 aatttctttc tgcacgacat ccatcaaaat caggtcggga ggttcgccgg cgagttgggc
     8641 ccagaattcg atcaggttgc tggcagtgac taccttgaac tcaccctgaa gcagctgccg
     8701 caggtagagt atcgctgtct ctgagtcatc gacaatgagt acaagcggca ggtgcgatgc
     8761 ggtgttctct gccacgggca tgagatgagt gtataccgtc gcgatggcat gtaaatcggt
     8821 tttggggtat acctcctaca gtttatctat gggctctttg tccttatgca gcgggtcgcg
     8881 caccaattac cagtctccca aaaatgtgga tttacaacgg caggtcgata atcaccgtcc
     8941 ctctgtatgc cccacggcag tcgacgcatc ttttcaccgg gagaaaccat cttcagcgaa
     9001 ggtgagcggc aggatatcgc cttcatcatc gaacagggtg aagtagaaat ctggaccaca
     9061 atcgaagatg aaaaaagaac gctgaatatc ctgaaggcgg gtgcactttt cggcgagctg
     9121 gcgctggttg accggcagcc gcgttctgca tcggcgactg cgctcggcga aacagtgctc
     9181 actgtggtca cgcagcagca ggttgacgag cggctggcag atgctgatcc cattctgcgc
     9241 atggtgcttt ttgtcgtgat gcggcatttc cgttcggagc tgcagcgcgc acgcggtcac
     9301 tacggcccct atgaccgtaa ccgcgaaact ccagcaccgg ttgagaatgc acaaaagcgg
     9361 atcgatgaag cgattgagct gatcaagata gaggcggagc tcaagagcgc gatgcagaac
     9421 gaagagtttc gcctgatgta ccagccgatt cacgaattga agacacagaa gattgcaggc
     9481 cttgaagcgc tcattcgctg gaagagcccg acgcgcggcg agatcatgcc ttcggctttt
     9541 atgccgcttg ccgagtcgac ttcgctgatt gtaccgattg gcaactgggt gatcgatgca
     9601 gggctcgccg attacctgca atttcgcgaa gcagctaagg cagatattac cctgagtttc
     9661 aacctggcgc gccggcaaat ggaacacggc ggatttctcc cttacttgac cgaacgtgtc
     9721 gacaggcatg gcattgcgcc gcatgctatc aaactcgaaa tgctcgagcg taatcttttt
     9781 gaatcggata cggtaaccgg ttggatacaa gactgtgtcg ctcgcggctt cagcattttg
     9841 ctcgatgatt ttggtacagg ttattcgagc ctgcagtacc tgcaggaata ccagcccgcg
     9901 gcgctgaaga tagaccgctc gttcgtgaag ggccttggcc agcgtgtcga gagtgaccgt
     9961 atctgccgcg cgataatcga gctggcgcac gcgctcggta ttggcgttat tgccgaggga
    10021 gtagagacgt tgcagcaggc gcgcatactg acagacatgg gctgccagca ggcgcagggc
    10081 tatttctttt cgccgccgct ttcagccgat gcgatacggc agcggctgag tgcctagcag
    10141 ctgcatctta cttggcaatg aatctgtcct tgttccagat aaactgtttt tttgtgacgg
    10201 catagtagtc ggtcggcttt ttcttgaaat ctgtttcgaa tacggaaacc gatccgtaca
    10261 ccatgcattt aggagaatac acacaagtga tattcagacc ttttcgcggc acagggcagt
    10321 cgaacccccg gggaaactcc atctgttgag gcgaagagct tgtatcctcc gcaccgcaga
    10381 gtttgggcca ttttgccttt ggcttaaaca gtgatttctg gaagtcgccg aaaacattgg
    10441 ccgtcacgtt cttccagtta ccgttctgtt gggtgaaaaa agtcgggctg ccatcaccat
    10501 tgctgacacc tatgatgtct tcgcccttat cggtgcgcca gacggccagc gccagcgttc
    10561 caggggccgg atccgcgcta tacttaattg ccaggtagcc gtttgcagca tcgtcgatca
    10621 cctcttcgac ctcgaacatg agcccaaggc cgtccgtatt gcgcgccagc accttcttgc
    10681 gcacatcgac gctctccatg cccatcagca gcgcatcggc aggcaataga aagaagtatt
    10741 ctttaatcgt ggttttgggt tttttggctc ctgttgccgt gacggcaaag caggcgagca
    10801 acagtagggt tatttttcgc atatggactc cttaggctat ttcgcgacga agcgatcttt
    10861 cttccattca aatttcacta cgggtttcgc atagaaatcc ttcggcgtta gttttgtggg
    10921 agtgtcacag ttcaggttaa actggcagac cacggtcagc cctttctgcg gcagtttaca
    10981 cgaaaagttt tccggaaact ccgcaagtgg cggttcatcc ttacagccag ctggggcgtt
    11041 ggcttttggt tgaaatgtgg cccggttgaa atcagcgaag acttctgggg ttatgatttt
    11101 ccacctaata cccggatacg cttcatcgag tgtaatgaag tacaacgtac ttcttatacc
    11161 tccgacccct gaatataaag aatttacacc tactacgggt ttatcccccg gatgacgcca
    11221 catcgcaaat tcgtgtcttg cggattgctg ccgggtcttg aagcccagcg ccatatagcc
    11281 attcttgtcg tcttgaacta taagtccgat cgtctcgtcg ctgccggctt tacgtcttag
    11341 tgcggcagtg cgatcctcaa attcttttaa accgtcgggc attacttcat cggggaaagt
    11401 ccaaaaatac gatgatatgg tggatttgct tcccactgca taggccagcg ataccactaa
    11461 caagaacagt attattcgtt tagtccacat agacagactc cagcggacag actgatgaag
    11521 tgcccagcat tttttctcag cgggtccgga tcggcaaagt atcacagccc tcgtgcgccc
    11581 tgcgagatct gcacttcgta aaccagcggc gcatgcccaa actgagcagc gaagcgtgat
    11641 tctgccgcgc tgataaaccc gggcagaccc gattcttcaa ccagattgat cgtacacccg
    11701 ccaaacccac cgcccatcat gcgcgcgccc gtgacaccgc agtcttttgc gattgcgacg
    11761 agaagatcga gctccgtgca gctcaccgca tacttgcggc ttaagccttc gtggctcgca
    11821 tacattagtc ggccaaatgc tgcggcatcg gcctgtttca gctgatcaac agcttcgact
    11881 acgcgatcat tttcttcaat cacatactca cagcgtgagt atgttgacgc atccagttcg
    11941 ccgcgaactg tcgcgagcag gacaagattc gcatcgcgca gactctgcac cgcagcatac
    12001 tttttgcgca ggaccgcgat tcccgcttcg cattcggcgc ggcgtgtgtt atactcagaa
    12061 ga
//
LOCUS       DAOQJF010000056        12041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2651_length_12041_cov_5.403721, whole genome shotgun sequence.
ACCESSION   DAOQJF010000056 DAOQJF010000000
VERSION     DAOQJF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12041
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2651_length_12041_cov_5.403721"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLF85_04655"
     CDS             <1..818
                     /locus_tag="PLF85_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNE18981.1"
                     /translation="KLHATEIAREAVKAGAELIVAVGGDGTISEVAAGFFDEKGKAIK
                     PVKQAPALGILPAGSGSDYAKTLGIPRASAIALQILQTKNTKTVDAGLVEFVGLNGQK
                     TKRIFINIADVGIGGEVVDILERQGKRLGAFLSYQLATLRGLIGYKNKNLEITIDKKQ
                     KFDGTYNGIIIAIGKYFGSGMKIAPNAEPDDGYFDVVMLGDMGKMEMVAKMQKVRNGS
                     HIFEKKVTVHRAKQISIRADARALLDCDGEMPGECPAEFSILPGALKVVVPPT"
     gene            complement(775..1176)
                     /locus_tag="PLF85_04660"
     CDS             complement(775..1176)
                     /locus_tag="PLF85_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18982.1"
                     /translation="MYWLRPALLYLFITVLLCAGVWLFWGDAISQGLKSDNRHTRKEP
                     AGRLAGTVREKTPPPKKMKPVDSPVNTQQVAVSGKEAWDKASSGFGLLLQTAGGENRR
                     LLLESVAEQDQRYRAMFPKLVERQPSAHQVK"
     gene            complement(1248..2984)
                     /locus_tag="PLF85_04665"
     CDS             complement(1248..2984)
                     /locus_tag="PLF85_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18983.1"
                     /translation="MSFCRAGALAAYSLLLSACSQYGDSVDQLSEELSKAIEQPAKKL
                     SRAELDEELEIGTISVLNEKVLPPADFFAPLINNLHYVTTRETIAAELNFKSGSKVRR
                     WELYDAERYIRMLDPIKQAKIIATPNKETGKTDITVLTQDRLSAYVRGGGAGSGGYSS
                     FGVQAGETSLFGRLYNVGASYTRENFRDFVGLSAGKQRIAGTRWEISGSSVDGFIGSA
                     HNYTGKGMSISHPFLVDGQRHSFSLSATYIQGISYEYLGGGIRQGLDTATGKSFDLIH
                     RQRVETISAEYLHGIGKKTRIEFGPGFLHYISKDYFVYPEDQYTVGDTKERVVSERSR
                     NFYQYEQYSSRAVSFAVNTRFGDFVPMMNFRRYLFTEDQFEGFRTSARVTHADPAFGL
                     EDHYTKTSAAATYSDNYRQKTFRLEAGIGRTATFWRQRYEIPRDDAWNMDVRFFHFVR
                     YGTLAVRQYVSSGNNMSIGARNQIAGEFTRGFLYGSVTPSAGVLSSVEYRSPALKLPY
                     LLLAGVAFFDYAGVGTGMQNLQWNPIVGLGLRSMLYEFDNNVFRLDIGYNLNDPEFSL
                     LNSLQFGMSHTF"
     gene            complement(2988..3665)
                     /locus_tag="PLF85_04670"
     CDS             complement(2988..3665)
                     /locus_tag="PLF85_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18984.1"
                     /translation="MLSLAERIDSAIQKNQTIKVRAYALLPETEKDLNLIILRLLNKY
                     NKLDMTATLYTCIKELAVNGAKANIKRILFDEVKIDLENESDYRRGLEIFREKLTDKF
                     INEYAQKAKDRGLYVDIKFEYDDNRLTVEVVNNSALTKREDERIREKFRDGLKYDNIG
                     ELYMDMQDNQEGAGMGIALILMMLKSEGIDPHFFTIYSDYQHKTSAKIVFPMVANFQG
                     QRADFNK"
     gene            3715..3924
                     /locus_tag="PLF85_04675"
     CDS             3715..3924
                     /locus_tag="PLF85_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18985.1"
                     /translation="MLQGSWVSADHRNMRIGGDFLLQSQLVELPADLRQLQLRFTEKM
                     LKATIAQKALEQSVRATPAALDTYA"
     gene            3970..4230
                     /gene="rpmA"
                     /locus_tag="PLF85_04680"
     CDS             3970..4230
                     /gene="rpmA"
                     /locus_tag="PLF85_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNE18986.1"
                     /translation="MAHKKGGGSSKNGRDSNSQRLGLKAFGGEFVKAGSILVRQRGTV
                     FHAGKNVLRAKDDSLFTTVPGVVKFERIDKNRKKISVYPSAN"
     gene            4399..5136
                     /locus_tag="PLF85_04685"
     CDS             4399..5136
                     /locus_tag="PLF85_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE18987.1"
                     /translation="MPATRKKPRQPEAKTREAILLAAIEEFAERGFAGARTENIARQA
                     NVNKAMLHYYYHDKQTLYLAVLEYLYGSSSDAEVLVERLTNAPLNSVQMVRVFLIVIL
                     RKHADERSDPFRRILAWELAAGENNLEAVAQKYMVPRIIGMCEAIRRGISAGELKCSN
                     PTLAVWSLISQVAFYFMHRKTYEGSSIYEELYENVSYDDMLNFLLGNFIASYATDKNI
                     SSDIPPEIARLADELAEKLVLPGLTGT"
     gene            complement(5113..6216)
                     /locus_tag="PLF85_04690"
     CDS             complement(5113..6216)
                     /locus_tag="PLF85_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNE18988.1"
                     /translation="MLPGFPELSTSVENGRYICRHGNTSLIFYSERSAARDLQQVSSM
                     LSGVGDSITLIVAPYSPAFAPLFDSDKRFYFLSPASFTDQLPQDKRLVSDEESLTLFI
                     NTVEVEDNFAVQILPQWQHLIPAIGQATRDVLARAAVRLKTIRHFGRLWPINFRLNLP
                     LLRDMPDIRQLQAAPDLLALAGPSLYPLPHIKPETSIWCADTALPVLLHHGIRPAVIF
                     SLDAGFASQEHFCGSEAFVRSGGVALVADALCSPPVLRLPFLQKYTYASSHPLVQEFC
                     NNLRPDLTPVSNPEGNVGNLMLAICSALFPDARPRILGHDGGHRRYVTHARGTAYYRR
                     SFAGSNRLGGIEAYSHRLGRRYGAAPLRAGEPG"
     gene            complement(6216..6710)
                     /locus_tag="PLF85_04695"
     CDS             complement(6216..6710)
                     /locus_tag="PLF85_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18989.1"
                     /translation="MRKIFLLLLFAIACKARGEQNVFSFCRDFSEMACHEPFSGKATY
                     PLEKSMKSKTMRDFWNSVYFRGDRLAFEVRNADARRTVDFDCLYGNYYLDGNTAEHHE
                     LEYIELKEKNVYGLVMLGSLLEKRFAPRKSEAYRPPEPFTVTYSVFCRNDLLARASIT
                     IEMR"
     gene            complement(6707..7162)
                     /locus_tag="PLF85_04700"
     CDS             complement(6707..7162)
                     /locus_tag="PLF85_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18990.1"
                     /translation="MTYFPQLLSAFHWLGVALYCGALFFFIIIFQRVYERYRAYRYVD
                     NFRGEVVLQLWRLLHVCFVVIVLSGAALAGLKGKSVLTGLYGLVFSSKLALWLLQLWL
                     LQDLLKPFLTENYTEEPQARSREWEARSLGAVLLLFLIAAGGFLLKYLP"
     gene            complement(7159..7530)
                     /locus_tag="PLF85_04705"
     CDS             complement(7159..7530)
                     /locus_tag="PLF85_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE18991.1"
                     /translation="MFSAKDMVIIETLSHINMPAKNLQKAVEFYTQFLDFEVVEEGNN
                     FAIVSFDNLHLKLDTGLEAYAKVPVLSFLLDVDDFTEALQEIEENNIEIAKGPFEVEG
                     GESVYVLDPSGNLVELYYRED"
     gene            complement(7579..8835)
                     /locus_tag="PLF85_04710"
     CDS             complement(7579..8835)
                     /locus_tag="PLF85_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE18992.1"
                     /translation="MADAHTNTVLEFVDWLAGDTGKTSSIPELFSEFNTFLNQHHYEI
                     FRANFASRSLHPQFGAVSLIWSERNDIPLPPAGSGILERRQMAVGKGFVTLLRIQRVN
                     IETQAFRDSPFFPVLTRGETVRERIAAGAAEFRFPIIKDLAELGGTDYLAMPVHFKGQ
                     IEGFFSFATRQGGGYSEKQLAELARMARLFSVALEARLRDDLLDTLLRLYLGEITGPQ
                     VLKGRIQRGDMESFESAVWFSDIRGFSALTSISTPQEIIELLNTYYETIIPVVRRYGG
                     EVLKFLGDGLLVIFGGREGKERQARYQSLLAANAAGKALQRLNEQRIAEEKIPLEHGI
                     GLHYGRIEYGNIGSDERLDFTAVGAAVNTASRVAALCADLGQSVLISGDLAQLLRVRL
                     TPQGIKELKGIPRPVAIFALPPKLAI"
     gene            9064..10419
                     /locus_tag="PLF85_04715"
     CDS             9064..10419
                     /locus_tag="PLF85_04715"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNE18993.1"
                     /translation="MKTKLTAILASLTVLALGLGAQAKKPKAPAAPAKPAVAAPAAPA
                     APAPAAPAAAAEEPAAPAEGFFVKSVKAGDGKDITLKLGGYVDTYYAFHSQGGDEIRA
                     QNHPNGTGGANGGTDLGSSATRGTNGRIFEGTNQQFTLGLVQTKFEIGNEDWQVVADL
                     VHGPNAELTNFNNLRGGSSSTTSTAIKQAYVAGNLAKGLKLTAGQFGTHIGYEVVESY
                     QNANYMLGYLFGYGPFYHTGAKLDYAISDKIAVMAGVVNGWDLQYDNNKNKTVIAQVT
                     LKPISELTVILNYAGGNETNTTASGSAATPPVADAKGMRHIGDVVVQYQVSKMIKVGA
                     NFVYGEEKVNEATAAQKWGGAAGYLSVTPIDGFTLSYRLDYFEDKMRARGLLGVTGGT
                     SNGATVMGHTLTGTISMYGGHFLIRPEVKYDRADLEVFGFSAEKKKDNVTALIAFTGV
                     Y"
     gene            10513..>12041
                     /locus_tag="PLF85_04720"
     CDS             10513..>12041
                     /locus_tag="PLF85_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE18994.1"
                     /translation="MSEKGRLKYVVSILVVVATAIGLFSTYRQYKAGLREKEQLLYAQ
                     VRSLSSLIEAVARFDRGTFRQRDKATAATLSQLFDAFADRIDFDTAQEIQFVRRSGHD
                     IEIFLRYFGRNKNQNIIVPDRKFLPEPVRRAVDGQSGAGYFTGLHGEDVLAAYTYIEP
                     LQIGLIVDLDMWGLQKPYLVSGAVSALFAIALLIIAALAIIRITNPIIARIREKDVLN
                     RTIVETAHDALIILDGEGNVRLANPATRSIFGWEPESLYERYFSVLFPPESKENIQLA
                     AFIRDDAAADLELTEAFGRKTDGSLFPARISANKKEYGHGKWCTLMIQDLSRLRAAER
                     TIRSLSRRLLEIQDIERDQISRDLHDSLGSTLVGLKLQVQSLIAHGPADANDLRQQVV
                     ENFNEAIEQTRNLSRLLSPFSVRHLDLGGALKQLAQSLASSREIVINAEIGKKDRVLS
                     PSARIQAYRIAQEAVQNAIKHSGARRITISLVQNGTELVMTIHDDGQGFDTAGAGGGQ
                     GL"
BASE COUNT         2801 a         3211 c         3315 g         2714 t
ORIGIN      
        1 gcaagctcca cgcgaccgaa attgcccgtg aggcggttaa agccggtgca gagcttatcg
       61 ttgcagtggg cggcgacggc acgatcagcg aagtcgccgc cgggtttttt gacgagaagg
      121 gcaaggcgat aaaacctgtt aaacaggcgc ctgcgctggg cattctgcct gcgggttcgg
      181 gcagcgacta tgcgaagaca ctcggtattc cccgcgcttc ggcaattgcc ttacagattc
      241 tgcagacaaa gaatacgaaa accgtcgatg ccgggcttgt tgagtttgtg ggactcaatg
      301 gccagaagac gaaacgtata ttcattaata ttgcggatgt gggtattggt ggcgaggttg
      361 tcgacatcct ggagcgccag ggcaaaagac tcggagcctt tctttcttac cagctggcga
      421 cgttgcgtgg cctgattggt tacaagaaca agaatcttga aataaccatc gacaagaagc
      481 aaaagttcga tggcacctac aacggtatca tcattgcgat cggaaaatac tttggcagcg
      541 gcatgaaaat tgcgccaaat gcggaacctg acgatggtta tttcgatgtg gtaatgctcg
      601 gcgatatggg caagatggaa atggttgcga agatgcaaaa agtccgtaat ggctcgcata
      661 tctttgaaaa aaaagtaacc gttcaccgcg cgaaacagat cagcatccgc gccgacgcgc
      721 gtgcactgct cgactgcgac ggtgaaatgc ctggcgagtg cccggcagag ttttctattt
      781 tacctggtgc gctgaaggtt gtcgttccac caacttaggg aacattgcgc ggtatcgctg
      841 gtcttgctcg gctacacttt cgagcaagag ccggcggttc tctccgccgg ccgtctgcag
      901 caaaagcccg aaacccgaac tggccttatc ccaggcttct ttaccagata cggcaacctg
      961 ttgtgtattg accggtgaat cgaccggctt catttttttg ggtgggggag ttttctctct
     1021 tacggtacct gccagcctgc ctgccggctc tttccgagta tgccgattat cgctttttag
     1081 tccctggctg atcgcatcgc cccagaagag ccaaacgccg gcgcagagta acacggttat
     1141 aaagaggtag agcagcgccg ggcgcagcca gtacatacgc acgcatgcag cctcagctta
     1201 cgctgcgtca agttgatagc gatttgggct cgtgctgcag gagcccgtta aaacgtgtgg
     1261 ctcatgccaa actgcagtga attgagcagg ctgaattcgg ggtcgttcag gttgtaaccg
     1321 atgtcgagcc gaaagacatt gttgtcgaat tcatagagca tcgacctcag gccgagaccc
     1381 acaatcggat tccactgcag gttctgcatg cctgttccca cgccggcata gtcgaagaag
     1441 gcgacgcctg ccagcaacag gtacggcaat ttcagcgctg gcgaccggta ctccactgac
     1501 gagagaactc cggcgctcgg ggttaccgag ccatataaaa agccccgcgt aaattcaccc
     1561 gcgatctggt tacgcgcacc gatactcata ttgttgcccg acgagacata ctgccgtact
     1621 gcgagcgtac cgtagcggac aaaatggaag aagcgcacgt ccatgttcca cgcgtcgtcg
     1681 cgcggaattt cgtagcgctg ccgccaaaac gttgcggtgc gaccgatacc ggcttcgagc
     1741 cggaaagttt tctgccggta gttatcgctg taggtagcag ctgcagaagt cttggtgtag
     1801 tggtcttcaa gcccgaacgc ggggtctgca tgcgtaaccc gggcagaggt gcgaaagccc
     1861 tcgaactggt cttcggtaaa aaggtagcga cgaaagttca tcatcggcac gaagtccccg
     1921 aagcgggtgt tcaccgcgaa gctcacggcg cggctggaat actgttcata ctggtaaaaa
     1981 ttgcgcgatc gttctgaaac cacgcgttct ttggtgtccc ccacagtgta ctggtcctcg
     2041 gggtagacaa agtagtcctt gctgatgtag tgcaggaaac ccgggccgaa ctcgatgcgt
     2101 gtctttttgc caatgccgtg caggtattcg gcagagatcg tttcgacgcg ctggcggtgt
     2161 atcaggtcga aggattttcc cgtcgccgta tcgagcccct ggcgaatacc tccaccgagg
     2221 tattcatagc tgattccctg tatgtacgtg gcactcaggc taaagctatg ccgctgccca
     2281 tcgacaagaa agggatgtga aatgctcatg ccctttcccg tgtaattatg cgccgacccg
     2341 ataaaaccat cgacgctgct gcctgaaatt tcccagcggg tgccggcgat acgctgcttg
     2401 cctgcactca acccgacgaa gtcgcggaaa ttctcgcgcg tgtaacttgc tccgacatta
     2461 tagagccgcc cgaacaggct ggtttcgcct gcctgaacgc caaacgagct gtaaccgcca
     2521 gaaccggcgc cgcctccgcg cacgtaggct gacagcctgt cctgcgtcag cacggtaata
     2581 tcggtcttgc cagtctcttt gttaggcgtt gcgataattt ttgcctgctt gatcgggtcg
     2641 agcatgcgga tataacgctc cgcatcgtac agctcccacc gcctgacctt gctgccactc
     2701 ttgaagttga gctctgcagc aatcgtctcg cgggtagtga cgtagtgcag gttgttaatg
     2761 agcggcgcga aaaaatcggc cggcggcagc actttttcgt tcaggactga aatggtacca
     2821 atctcaagtt cttcgtcgag ttcggcgcgc gacagttttt tagcgggctg ttcgattgcc
     2881 tttgataatt cttcagatag ctggtcgaca gagtcgccgt actgcgagca ggcacttagc
     2941 agcaggctgt atgcggcgag agcgccggct ctacaaaagc tcacacgtca cttgttaaag
     3001 tctgcccgct ggccctgaaa atttgcgacc ataggaaaga caatcttggc gctggttttg
     3061 tgctggtaat cgctgtagat agtaaaaaaa tgaggatcaa taccttctga cttcagcatc
     3121 atgagaatca gggcgatacc cataccggca ccttcctggt tgtcctgcat gtccatgtag
     3181 agttcgccga tgttgtcgta tttgagcccg tcgcgaaact tctcgcgtat acgctcatct
     3241 tcgcgtttgg tgagcgcgct gttgttcaca acctcgacgg taagcctgtt gtcgtcgtat
     3301 tcaaacttga tatcgacgta aagtccccgg tctttggcct tctgggcata ctcgttgatg
     3361 aacttgtcgg tcagcttttc gcggaatatc tcgagccccc ggcggtagtc cgattcgttc
     3421 tcgagatcaa tctttacctc gtcgaacagg atgcgcttaa tattggcctt ggcgccgttg
     3481 acggcgagct ctttgatgca cgtgtagagc gtcgcggtca tgtcgagctt gttgtattta
     3541 ttcagcagcc gcagaatgat caggttgaga tctttttcag tctctggcag cagggcgtac
     3601 gccctgacct ttatggtctg gttcttctgt atggcactgt caatgcgctc tgccagcgaa
     3661 agcatggggt cggcctgcga caggcggctg aaatagcaag ccgaaaggta aaaaatgctt
     3721 cagggttctt gggtatctgc cgaccacagg aacatgagga taggtggcga cttcttactg
     3781 cagtcccagc tggtcgaatt gccggctgac ctgaggcagc tacagctccg ctttaccgaa
     3841 aaaatgctga aagcgaccat tgcgcagaaa gcattagagc agagcgttag ggctaccccg
     3901 gcggcgctgg atacgtacgc ataagcccat aagggcttta cgtcccgttt cgaatcggca
     3961 gactggcata tggcacacaa gaaaggcggc ggttcctcga aaaacggccg tgactcaaac
     4021 tcgcagcgcc tcggtctgaa ggcgtttggc ggcgaattcg taaaagcagg ctctatcctg
     4081 gttcgccagc gcggtactgt tttccatgca ggcaagaacg ttctgcgggc aaaagatgac
     4141 tcacttttca ctactgttcc cggtgttgtg aaattcgagc gcatcgataa gaaccgcaag
     4201 aaaatctccg tttatccctc ggcgaactga gctgggcgga atagaccgat aagggccctt
     4261 cggggccttt ttttttgccc tgttggccca gcagcggcta tcgtttgcat atagcccggg
     4321 ctctgcattg acaagttgtt aaccgtttgg ttaatttttt tggtaatctc cggcgctcct
     4381 cgttatttta caggtaccat gccggctacc cggaaaaagc cacgccagcc tgaagccaag
     4441 acccgcgaag ctattctgtt ggcggccatt gaagaattcg cagagcgcgg tttcgccggg
     4501 gcgcgcacgg aaaatattgc ccgccaggcc aacgtcaata aggcaatgct gcactattat
     4561 taccatgata agcagactct ttacctcgca gttctggaat acctctacgg ttcgtcgtcc
     4621 gatgctgaag tgctggtgga gcgactgacg aatgccccgc tcaactcggt acaaatggta
     4681 cgcgttttcc tcattgtcat tttgcgaaag catgctgatg agcgcagtga cccattccgc
     4741 agaatacttg catgggaact tgcagcgggt gaaaacaatc ttgaagctgt tgcacaaaag
     4801 tacatggttc cgcgcatcat cggcatgtgc gaggccatca ggcgcggcat ctctgcgggg
     4861 gagctaaaat gttcaaaccc gactctcgcc gtctggagcc ttatttcgca ggtcgcattc
     4921 tattttatgc accgtaagac ttatgaaggt agctcgatct atgaagaact ttatgagaat
     4981 gtgtcatacg acgatatgct gaactttctg ctgggtaatt ttatcgcttc atacgcgaca
     5041 gacaagaaca ttagttcgga tatccctcct gaaattgcgc ggctcgccga tgaactggcc
     5101 gagaagcttg tgctacccgg gctcaccggc acgtaaaggt gctgcaccgt agcgtctacc
     5161 caggcggtgc gagtaagctt cgataccacc caaccggtta gagcctgcaa atgagcgcct
     5221 gtagtatgca gttccacgcg catgtgttac gtatctgcgg tggccaccgt cgtggccgag
     5281 aatgcgtggc cgggcgtccg ggaaaagcgc agagcagata gcaagcatca ggttgccgac
     5341 attgccttcg ggattgctca caggcgtcag gtcagggcgc aggttattgc aaaactcctg
     5401 caccagcgga tgcgagctcg cgtacgtgta tttctgcaga aaaggcaggc gcagcaccgg
     5461 aggtgaacag agagcatctg ccaccagtgc aacaccgccg ctgcgtacaa aggcttcact
     5521 gccgcagaaa tgttcctggc tggcaaaacc tgcatcgagc gaaaaaatta ccgccgggcg
     5581 aatgccgtgg tgcagcagca caggcagtgc tgtatcggcg caccagatgc tggtttccgg
     5641 cttaatgtgc ggcagcggat agagcgaagg gccggcgagc gccagcaggt cgggtgctgc
     5701 ctgtagctgc ctgatatcag gcatgtctcg caacaagggc aggttgaggc gaaagttgat
     5761 cggccacagc cggccaaaat gcctgatcgt cttgagccgc acggccgcac gtgccagcac
     5821 gtcgcgggtt gcctgcccga tggcaggtat caggtgctgc cattgcggta agatctgcac
     5881 cgcaaaattg tcctccacct caaccgtatt gatgaagagt gtaagacttt cttcgtcgct
     5941 gaccaggcgc ttgtcttgcg gtaactggtc ggtgaaactt gccggagaca aaaagtagaa
     6001 tcttttgtct gaatcgaaaa ggggggcaaa tgcaggcgag tagggcgcca caatcagggt
     6061 aatgctgtcg ccgactccgg acagcattga gctgacctgc tgcaggtcgc gtgcggcgga
     6121 acgttcgctg taaaagatca ggctggtgtt gccgtgtcgg caaatgtagc gcccgttctc
     6181 aacgcttgtg ctcagttcgg gaaaaccggg cagcatcagc gcatctctat ggtaatcgaa
     6241 gcgcgcgcca gcaggtcgtt gcggcagaag acggagtagg tcacggtaaa tggttcgggt
     6301 ggtctatagg cttcactctt gcgaggcgcg aagcgctttt cgagcaggct gcccagcatg
     6361 accagcccat agacattttt ttcttttagt tcaatatact cgagttcatg gtgttcagcg
     6421 gtattgccgt ccaggtagta attgccgtac aggcagtcga aatccaccgt gcggcgcgca
     6481 tcggcattgc gtacttcaaa ggccagccga tcgccacgga aatagactga attccagaaa
     6541 tcgcgcatgg tcttcgactt catgcttttt tccagcgggt aagttgcctt gccagagaac
     6601 ggttcgtggc acgccatttc actgaagtcg cggcaaaacg aaaatacgtt ttgctcgccg
     6661 cgcgccttac aggctatcgc gaacagcaac aggagaaata tttttctcat ggcaggtact
     6721 tcagaaggaa accgccggcc gcaatcaaga acaaaagcag caccgcccct aaagaccgtg
     6781 cctcccattc gcgggaacga gcctgtggtt cttctgtgta attttcggtt aagaagggtt
     6841 tgagcaggtc ttgcagcaac cagagctgca gaagccagag cgccagtttg ctcgaaaaga
     6901 ccaggccgta gaggccagtg agtaccgact tgcccttgag gcccgccagc gctgcgccgc
     6961 tcagcacaat cacgacaaag cagacatgca gaaggcgcca gagctgcagc accacttcgc
     7021 cgcgaaaatt atcgacgtag cggtaggccc tgtagcgttc gtagaccctt tggaatatta
     7081 taatgaagaa aaaaagagct ccgcagtaga gcgctacgcc cagccaatgg aatgccgaga
     7141 gtagctgcgg aaaataggtc aatcttcgcg gtagtagagt tcgacgaggt taccgctcgg
     7201 gtctaatacg tagacgcttt cgccaccctc gacctcaaac gggcctttag caatttcaat
     7261 attgttttct tctatttcct gcagtgcctc ggtgaaatcg tcaacatcga gcaggaaaga
     7321 gagtacagga actttggcat aggcctcgag gcctgtatcg agcttcaggt gcaggttatc
     7381 aaatgaaaca attgcgaagt tattgccttc ttctacgacc tcgaagtcca ggaactgggt
     7441 gtagaattct accgccttct gcagattttt cgccggcata ttgatgtggc tgagtgtttc
     7501 gatgatgacc atgtccttgg cagaaaacac gggaaaaaca ggttatcaag tcatttttga
     7561 aaagactgaa catggtgttc aaatagccag ttttgggggc agggcaaaaa tcgccaccgg
     7621 gcgtggaatt cccttgagtt cttttatacc ctgtggcgtg agcctgaccc gtagcagctg
     7681 ggccagatcg cccgaaatca gtaccgactg gcccaggtcg gcgcagagcg ccgccacccg
     7741 gcttgcggta ttgaccgcgg caccgaccgc ggtaaaatca agccgctcgt ctgaaccgat
     7801 gttgccgtat tcgatacggc cgtagtgcag gccgatgccg tgttcgagcg gaattttttc
     7861 ttcggcaatg cgctgctcat tcagtctctg cagcgccttg cccgcggcat tggcggcgag
     7921 aagcgactgg tagcgcgcct ggcgttcttt gccctctctg ccgccgaaga tcacgagcag
     7981 gccgtcaccg agaaatttca gaacttcgcc cccgtagcgc ctgacaacag gaataatcgt
     8041 ttcgtaatac gtattcagca gttcgatgat ttcctgcggc gtactgatcg acgtcagcgc
     8101 ggaaaatccg cgaatgtcgg aaaaccagac ggcagattca aagctctcca tatcgccgcg
     8161 ctgtattctg cccttgagca cctgcgggcc ggtgatttct cccagataga ggcgcagcag
     8221 cgtatcgagc aggtcgtcgc gcaggcgggc ttcgagcgca acgctgaata ggcgcgccat
     8281 acgagcgagt tcggcgagct gcttttcact ataaccgcca ccctgccggg tggcaaagct
     8341 gaaaaagccc tcaatctggc ccttgaagtg caccggcatc gcgagataat ctgtaccgcc
     8401 gagctccgca aggtctttga tgatcggaaa gcggaattct gcggcgccgg cggcgatgcg
     8461 ctcgcgcacg gtttcgccgc gtgtcagcac aggaaaaaaa ggcgaatcgc gaaatgcctg
     8521 tgtttcgata ttcacgcgct gtattcgcag cagggtgaca aaacccttac cgacagccat
     8581 ttgcctgcgc tcgagaatac ccgaacccgc cggcggcagc ggtatatcgt tgcgctcaga
     8641 ccagatcagc gaaacggcac caaactgcgg gtgcagcgac cggctcgcaa agttagcgcg
     8701 aaaaatttca taatggtgct ggttgagaaa agtattgaat tcactgaaaa gttcaggaat
     8761 cgagctggtt tttccggtat cgccggcgag ccagtcgaca aactcgagaa cggtattcgt
     8821 gtgggcatct gccatagcgg gcgtcatttt tgtggggagc tatgagcctg atgcgggccc
     8881 tgtccggtca acagaattca tccgatcttt tcctattccg taactactaa actctccttt
     8941 aataaatcac cccgcccttg gtattgtctg gtagccaagg gggcagggat ggacatattt
     9001 ggcacgggag ttgctatata gccggcaaga ggacgaaagt cactcaacta taaggagttt
     9061 aagatgaaaa cgaaactgac agccattttg gcaagtttaa cggttctggc cctgggcctc
     9121 ggcgctcagg caaagaaacc aaaagcaccc gcagcgcccg cgaaacccgc agtagctgca
     9181 ccagcagcac ccgcagcacc ggctccagcc gcccctgcag ctgcagcaga agaaccagca
     9241 gcacccgctg aaggcttctt cgtgaagagt gtcaaggctg gcgacggtaa agacattacg
     9301 ctcaagctgg gtggatatgt agatacctac tatgcattcc acagccaggg cggtgatgaa
     9361 atccgtgcgc aaaaccatcc aaacggaaca ggtggtgcca atggtggtac agacctcgga
     9421 agctcagcta cacgtggtac caacggccgt atctttgaag gcacgaacca gcagtttacg
     9481 ctcggattgg ttcagaccaa gttcgaaatt ggcaatgaag actggcaagt cgtggccgac
     9541 ctcgtacacg gtccaaacgc cgagttaacg aacttcaaca acctgcgcgg tggatcgtcg
     9601 tcaacgacat cgacagcaat caagcaggcc tatgtggcag gtaacctggc aaaaggtctc
     9661 aagctcacag cgggtcagtt cggtacacac attggctacg aagtggttga atcttaccaa
     9721 aacgctaact acatgcttgg ttacctgttt ggatatggtc cattttacca caccggtgct
     9781 aaacttgact acgcaatctc agacaaaatt gctgtaatgg caggtgttgt caacggttgg
     9841 gacctccagt acgataacaa caagaataag actgtgattg cacaggtaac cctgaagcca
     9901 atctcagaac tgactgttat cctgaactat gctggtggta atgaaactaa cacaacagca
     9961 tctggatcag ctgctacgcc tcctgttgcc gacgccaaag gcatgcgcca catcggcgac
    10021 gtcgtagttc aataccaggt cagcaagatg atcaaagtcg gtgcaaactt tgtgtacggc
    10081 gaagaaaagg tgaacgaagc aactgcagct cagaaatggg gtggagcagc tggttacctg
    10141 agcgtcacac caattgatgg ttttactctc agctaccgtc tcgactactt cgaagacaaa
    10201 atgcgtgccc gtggcctcct cggcgtaact ggtggaacat caaacggtgc gactgtgatg
    10261 ggtcacactc tgaccggtac catctcaatg tacggcggtc acttcctgat ccgtccggaa
    10321 gtgaagtacg accgcgcaga tctcgaagta ttcggcttca gcgcagagaa gaagaaagac
    10381 aacgtcactg ctctgatcgc tttcactggc gtatactaag agtagatccg gctacggttc
    10441 tgccggttca aaaggggggc aacccccttt ttttattcgt ttacccatga tctcattttc
    10501 ccacgcagcg acgtgtctga aaagggccgc ctcaaatatg tcgtgtccat tctcgtagtc
    10561 gttgcgactg ccattggatt gttttccact taccggcagt acaaggcggg attacgcgaa
    10621 aaagagcagc tgctctacgc ccaggtgcgc agcttatctt ctttaataga ggccgtcgcc
    10681 cgcttcgatc gcggcacttt caggcaacgc gataaggcca ctgcggcaac cctcagccag
    10741 ctcttcgatg cctttgccga ccgtatagat ttcgatacgg cacaggagat ccagtttgtc
    10801 cggcgcagcg ggcatgacat tgaaattttc ctgcgctatt ttggtcgtaa taagaaccag
    10861 aatattatcg tccctgaccg caagtttttg cccgaaccgg tgcgccgcgc ggtagacggg
    10921 cagtctggcg ctggttactt tacgggtctg cacggtgaag atgtgctggc ggcatatacc
    10981 tacattgaac ctctgcaaat tggcctgatt gtcgacctcg acatgtgggg tctgcagaag
    11041 ccctacctgg tttccggagc tgtctcggcc ctgtttgcta ttgcgctgct gattattgcg
    11101 gcgctggcta ttatccgtat caccaatccc attatcgcgc gcatccgcga aaaagatgtg
    11161 ctcaatagaa caatcgttga aaccgcgcat gatgcgctga tcattcttga tggtgaaggc
    11221 aacgtccgcc tggcaaaccc ggcgacgcgc agcatttttg gctgggagcc cgagtcttta
    11281 tacgaacgct actttagtgt cctgtttcct cccgagagca aagaaaatat ccagctggca
    11341 gcattcatcc gcgacgatgc ggctgcggac cttgagctga cagaagcctt tggccgcaag
    11401 accgatggct cacttttccc tgcgcgcatt tctgccaaca agaaagaata cggtcacggc
    11461 aaatggtgta cgctgatgat ccaggacctg tcacgcctgc gcgcggccga gcggactatt
    11521 cgcagcctct cacgccgcct gctcgaaatc caggacatcg agcgcgacca gatttcccgc
    11581 gacctgcacg acagccttgg ctctacgctg gtcggtctta agctacaggt gcaatcactg
    11641 attgctcacg gccccgcgga cgcaaacgac ctcaggcagc aagtggtcga gaatttcaat
    11701 gaagccattg aacagacacg aaacctgtct cgcctgcttt caccattttc ggtgcgccac
    11761 ctggatcttg ggggggctct caagcagctt gcgcaatccc tagccagttc gcgagagatt
    11821 gtcatcaatg ccgagatcgg caagaaagac agagttcttt caccatcggc ccgcatacag
    11881 gcctatcgca ttgcccagga ggccgttcag aatgctatta aacattcagg ggcgagacgc
    11941 atcacaattt cgctagtcca gaatggcacg gaacttgtta tgacaataca tgacgacggc
    12001 cagggatttg acactgcagg tgccggtgga gggcaggggc t
//
LOCUS       DAOQJF010000057        11967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2670_length_11967_cov_4.776192, whole genome shotgun sequence.
ACCESSION   DAOQJF010000057 DAOQJF010000000
VERSION     DAOQJF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2670_length_11967_cov_4.776192"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLF85_04725"
     CDS             <1..715
                     /locus_tag="PLF85_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE18995.1"
                     /translation="EFTRELAGQAPYLAVYYSFVGHWPYPDLGLEHRLIEPRSSRDRY
                     INNLYAQDKVIRQIVESLEASGKMQNTVLVIVGDHGEAFYQHPGNRVHSAESYNENIA
                     APLFIYAPGLVEPRRIAAPTVHADIVPTVLDLLGRRYDGERFQGESALRGEYRRKYIF
                     TFGNENTITAVSGQLAKMQMLRASGNCRHFDLVLDPAEQKNLGCETGGEHYRALEAFL
                     KLQPGLLKGYNETCLNTGC"
     gene            complement(783..2033)
                     /gene="odhB"
                     /locus_tag="PLF85_04730"
     CDS             complement(783..2033)
                     /gene="odhB"
                     /locus_tag="PLF85_04730"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNE18996.1"
                     /translation="MADIEVKVPAMGESISEGTISRWFKKEGEAIKADEALFEIETDK
                     VTQEIYAPAGGVVKKLMKAQGANAAVGEAVAIIDSSATAAAASAPAAQPASPAAPAAP
                     EAKSMTSTVPPAAKKIAAENNIDTSSIAGSGKHGQVTKGDVLAAAAAKPAAPTASAPA
                     SAPPDSAQPITPSRPSLGNRERETIQPMTRLRRAIADRLVSAQHTAAILTTFNEVDMK
                     PAMDLRNRHNDEFQKRYGVKIGFMSLFTKAVVSALQEIPAINAEIRGNDVVYKNYYDI
                     GVAVGGPRGLVVPIVRNADQLNFAEIELEISRLAGRVKEGSITLPEMEGGTFTISNGG
                     IYGSMMSTPILNPPQSGILGMHNIVKRAVVVTENGVDKIEIRPMMYIALSYDHRIVDG
                     KEAVTFLVKVKQAIEDPVRLLLGV"
     gene            2177..3436
                     /gene="glyA"
                     /locus_tag="PLF85_04735"
     CDS             2177..3436
                     /gene="glyA"
                     /locus_tag="PLF85_04735"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNE18997.1"
                     /translation="MSYPILQQNDPELYAALQAEDDRQNSHLELIASENFVTRRVLEA
                     YTSTLTNKYAEGYPGKRYYGGCGPADMVETLAIERLKKIFNAKFANVQPHSGASANLA
                     AFYAVLQPGDTYMGMDLSHGGHLTHGSPVNFSGLYYKPVPYGVKEDTHLIDYDQVERL
                     ALEHKPKMIIAGASAYPRVIDFPRFRQIADKVGAKVMVDMAHIAGLVAAGEHPSPLEY
                     ADIVTTTTHKTLRGPRGGVILTNSEEMIKILNSRVFPGVQGGPLMHVIAAKAAAFAEA
                     LTPEFKTYQKQVVANARALAQRLVERKFSLVSGGTDNHLMIVNTFDTKGITGKDAQAM
                     LELANITTNKNMLPYDKNKPAVASGIRLGSPALTTRGFGESEFRETADIIADILEAKG
                     SEAAIADAKKRVAALCAAYPMDKFRLG"
     gene            3447..3884
                     /locus_tag="PLF85_04740"
     CDS             3447..3884
                     /locus_tag="PLF85_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE18998.1"
                     /translation="MPQKLLQVVMLACLVGGAALAADTRRKAIRREMIEMDIAVRNLT
                     STIAMADRKVLDDSLQRLVTWQIKDHPDLGAAFRQVLADWQSKNLSRYGTELQKEAQA
                     LRSFAAGRAGRLSDSDWAKIGSGLNRILINCQNCHEAARKEPK"
     gene            3885..4850
                     /gene="lepB"
                     /locus_tag="PLF85_04745"
     CDS             3885..4850
                     /gene="lepB"
                     /locus_tag="PLF85_04745"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNE18999.1"
                     /translation="MKKFYERYFSIHNWLRENRVVGSLVSLGGLVAFVFAIKTSVLDA
                     NNIPSPSMEPTLMIGDFLFVNKMRYTIDLPYTNIHLVRIAYPERGDIVTFTPPQDSAS
                     YANKTLVKRVVGVPGDTVEVEDHEITISGKKYPTTPANNEELLKTLADGNTGERERLY
                     EEKIIDPKTGRLVVAHYMMKSRIRATDMLMATPGRKWVIPAKKYLMMGDNRDHSSDGR
                     ACEMVEAKYRKACNDFNNCMYDTWSGQRTEKQCQAIYAGAKGETWGLIDADNIHGKVY
                     LSYLSVNWGTGMNSELNPIFNLWYTITGKFSGVYVRWERIFRRIY"
     gene            4871..6346
                     /locus_tag="PLF85_04750"
     CDS             4871..6346
                     /locus_tag="PLF85_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE19000.1"
                     /translation="MRRTLVSSLCLIVAGADLVAQPYLSSRIVSVRSAETGRIEITVR
                     SEKSGEFDAITPDNAPQFTVTENGRAQSSVQSELLPQHSQVSRTVILADLSHSLTRRQ
                     FADFKRAATDFVGRLKPGDLVALVTFHRRVYRELNFTGDRELLKKRVAALRQTGRRTM
                     LYDALIEAHKMLRDVPDRKAIVVYTDGKENASRVRFDDLTEVFTSQPVPLFVAGNKKS
                     FSLRKLIRLARVSGGDAFRAEDRHDLGKVFSYLSRLRAQEFRISYLTQQKPGSAIDLE
                     VRSPQWAQALSASYTIPHGVLAQAPVREKLFSSALPETIRAHMPEILLSLIVLLLIAV
                     IIMLFFRRQEISVKVENVQPPVFVGNDEILPPGLKRQETPRAKLPLDYHHGWLVEKEG
                     PHTGRKYRINWHTVTIGFADDCSIVIDDNTVSAKHAKIERQGNKFVLYDLLSENGTWL
                     NGKKLLRPKELNDFDEIGIGRTKLIFRKSAGKNEERHGEKA"
     gene            6318..7541
                     /locus_tag="PLF85_04755"
     CDS             6318..7541
                     /locus_tag="PLF85_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNE19001.1"
                     /translation="MKKDTAKKRKTTPVTKKNPYSIELRKLRLGKSEVILLGTAHVSR
                     ESVADVERAIVKEKPDAVLIELDEGRARNLRDPDHWKHTDIVQVIKSGKIYLLFSSIL
                     LSIFQKKMGDRLTSAPGAEFKKAIELSEQKKLAHYFVDREIRITLKRAWQSVGFFGKL
                     RLMSELIASLFVTEDTASEDIEKLKEKDALQTLLDSMPPSFRRIREIVIDERDYYMAQ
                     KVRDIVLHAEKKPKRALVVIGAGHLRGIEEAMQRDHDMQALTAVKEGSLWKSLVAFLA
                     PIAIIVAFLSYFTFSGGKKVDIAETLKIWVIIKCSVTAAITIIWRPHILAYLAGILVS
                     PVSTFLPVIKVGWVSALLEAMLRKPEVTDFEEMPEATTRFRAFYRNRIFRIFMVFFLG
                     QFSSALGYWVFFWYV"
     gene            complement(7514..8938)
                     /locus_tag="PLF85_04760"
     CDS             complement(7514..8938)
                     /locus_tag="PLF85_04760"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNE19002.1"
                     /translation="MARQIAGRTVHFIGIGGTAMAGAAQMAVELGFSVRGSDQNLYPP
                     TSTELKASGIPYTEGYAEQNLEYGPDLVVLGNAISRGNVELEYALAQRFEILSLPQFV
                     AEYVIGRRRSLVIAGTHGKTTTTSLAAHVLREAAIDTGYMIGGVAADFPRSAAVGSAD
                     LFVIEGDEYDTSIFDQRSKFLSYRPTHVILNNIEFDHGDIFADLRDVERTFARLIKII
                     PANGVLVTNADNASCLQLSRSSFAPVITFGASAAADYQLLELQTGKTTTIKSRVRGEI
                     FEFESPLFGRHNALNALACLALLESVGVSREQFKQAMLSFHGVKRRLELRLETAEVTV
                     IEDFAHHPTAIAANLQTLREIYPARRLVVLIEPRSNTMVRNIFQSELTDALANADVAF
                     IDQIYRPEKYRPEERLDTALLVKSLNERGRSAFLLPAENRVDTVRAQLQKGDIVCFMT
                     NGSFGGLIGALVDALAQTYQKKTQ"
     gene            9016..11178
                     /locus_tag="PLF85_04765"
     CDS             9016..11178
                     /locus_tag="PLF85_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19003.1"
                     /translation="MSQKSRAEEIARVTDTNGIKVPRIRLSMRWSIVSLLTLLIIFSA
                     GLIILVSYIGGRQSTQYLMDTLMEDKAYRFIDKTSQFLLQAHNLAEDAREEIAKLNTL
                     TQRRELEEELRDIFESQPYYSAAYWVQSGRAVVARHQAIPEAQATNLMDELDDEKHNA
                     AAHLKDLTFEDKQVPAGSEFPWQKKEYADGKKMMPLHRVFSQNAPGFTFCSPVNPARG
                     ISAGAVCVDITLGDLALYIGSVKVGETGRAFIADKNLNVLAAPQQDRTSSAYQVRQDS
                     TELKNLTVFGNAEFNAAYLAMNKKLKKLQKNKRWLKTRKPFYFDFKHGGSRYVGIFTQ
                     FPRYTGLDWQAGVIIPEDEFFYFVRRNTRIVIAASTLLVLLAVVLGYIFSRRLSRPLN
                     ELAHEMDKIQNFDLTSGKAIVSSITDVDNMVMSFYKMRQGLRSFGKFVPADIVRQLIA
                     QEGDAHLQGEKRQLTVHFSDIEGFTTVSESIPPEQLVELLAEYLGEMSRIIAEENGTV
                     DKYIGDAVMAFWGAPQAVENHAHCAARASLRCQKRLAELENKWQAEGKPVFRARIGLN
                     TGEIIVGNMGSAERLNYTVIGDAVNLASRLEGINKYYGTRIIVGETTQKLIADLYATR
                     LLDFVAVKGKSEAIRIYELVGEKHALDGTSASFIAHYEAAIGHYRNQQWDAAEKEFRA
                     ALAVRPDEAAEMMLGRIEEFRRESPPAGWNGEYVMSSK"
     gene            11225..>11967
                     /gene="dprA"
                     /locus_tag="PLF85_04770"
     CDS             11225..>11967
                     /gene="dprA"
                     /locus_tag="PLF85_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNE19004.1"
                     /translation="MAGRTLSEKNRRLLEAAGSLLRLAPVLLANEFAQFLGQARETLH
                     RLGTDTVVTLSDDDYPPLLAEISTAPFALFLRGNRRLIGAEKISIVGTREPSPTGRMA
                     ARNIVAHFAAQERVIVSGIARGIDAVAHHAALQAGGATIAILPNGFDHLYPLENRDIY
                     ELARTSDRVLLLSEYSPAEKPQRYQFVRRNRIIAGLSSTTVVIEAGIKSGAMITVNHA
                     LDEGRDVAALSHMSLLNNAGGEKLVADGA"
BASE COUNT         2751 a         3276 c         3368 g         2572 t
ORIGIN      
        1 ggagttcacc cgcgaattgg ctggccaggc cccttatctc gcggtctact acagctttgt
       61 cgggcactgg ccgtaccctg acctggggct ggagcaccgg ctcatcgaac cgcggtcatc
      121 gcgcgatcgt tatatcaata acctgtatgc gcaggacaag gtgatccggc agatcgtgga
      181 atcactcgag gcgagcggca aaatgcagaa cacggtgctg gtcatcgtcg gtgaccacgg
      241 cgaagctttt taccagcacc cgggcaaccg cgtgcattcc gctgaaagtt acaacgagaa
      301 cattgccgca ccgctcttca tctatgcacc ggggctggtc gaaccgcgca ggattgctgc
      361 accgaccgtg cacgccgaca tagtgcccac agtgctcgat ttgctgggtc gcaggtacga
      421 cggcgaaaga ttccagggtg agtcggcgct gcgcggtgaa taccgccgca aatacatttt
      481 cacttttggc aatgagaaca cgatcaccgc ggtctcgggg cagttagcga aaatgcagat
      541 gctgcgcgcc tcgggcaact gccggcactt tgacctggtt ttagatccgg ccgagcaaaa
      601 gaacctgggg tgtgaaaccg gcggcgaaca ttaccgtgca ctcgaggcat ttctcaagct
      661 gcaaccggga ctattgaaag gttacaacga aacctgcctc aacacaggct gctagtgggc
      721 ttctgagaag cagccggtgc gggagttttt ccgaaacctg ccagcagctt acggagctgc
      781 gatcagacgc cgagcagcag ccgtacagga tcttcaatcg cctgtttgac cttcacgagg
      841 aaggtcacgg cttctttgcc gtcgacaata cggtgatcgt atgacagcgc gatatacatc
      901 atcgggcgga tttcaatctt atcgacacca ttttccgtca ccacaactgc acgcttgacg
      961 atattgtgca tgccgagaat gccactctgt ggcgggttca gaatgggggt agacatcatc
     1021 gaaccgtaga taccgccgtt ggaaatcgta aaggtgccgc cttccatttc gggcagggta
     1081 atgctgcctt ctttgaccct gccagcgagg cgagagattt caagttcgat ttcagcgaaa
     1141 ttcagctggt ctgcgttgcg tacgatgggt accaccaggc cacgtggtcc acccactgcc
     1201 acgccgatgt cgtagtagtt cttgtagacg acatcgttac cgcggatttc agcattgatc
     1261 gctggaattt cctgcagggc gctcaccaca gccttggtga aaaggctcat gaagccgatt
     1321 ttgacaccgt aacgtttctg gaattcatcg ttatgccggt tgcgaagatc catcgccggc
     1381 ttcatgtcaa cttcgttgaa agttgtgagt atggctgccg tatgctgcgc agagacaagg
     1441 cggtctgcaa tcgcccggcg caggcgtgtc atcggctgaa tcgtctcgcg ctcgcggtta
     1501 ccgagcgaag gtcgtgatgg agtaattggc tgcgcggaat caggtggcgc gcttgccggg
     1561 gcagatgcag ttggagcagc tggtttagcc gccgcagcag cgagtacgtc gcccttggtt
     1621 acctggccgt gtttgcccga accggcaatc gagctggtat cgatattgtt ctccgccgca
     1681 atttttttcg ccgcaggcgg cacggtgctg gtcatacttt ttgcttcggg cgctgcaggg
     1741 gcagccgggc tggcaggttg cgccgctggc gcagatgctg cggccgcagt ggcggaagaa
     1801 tcgataatcg cgacggcttc acctaccgca gcgttcgctc cctgtgcttt catgagcttt
     1861 ttgacaacgc cgcctgcagg tgcataaatc tcctgcgtga ccttgtctgt ctcaatttca
     1921 aagagcgctt cgtctgcctt gatggcctcg ccttcttttt taaaccagcg tgagatagta
     1981 ccctcgctga tcgattcgcc cattgcgggg actttgactt ctatatccgc catggttggg
     2041 tgacctaact ttgagctata gcgtcaagag attcgatatt cttctgtgac cgtttcaggc
     2101 cacagaaggc catcactgct tcggaaggtg caggaatttc gtttgcggca tgactaaagt
     2161 tccgcttttg agcgttatgt cgtaccccat tctgcagcaa aatgacccgg agctctacgc
     2221 ggctctgcag gctgaggacg acaggcagaa ctcgcacctc gagctgattg cctcagaaaa
     2281 cttcgtcacc cggcgggtac tcgaggcgta tacctcgacg ctgaccaata agtacgccga
     2341 aggttacccg ggcaaacgct actacggtgg ctgtgggccg gcagatatgg tcgagaccct
     2401 ggcaatcgaa cgactcaaga agatattcaa tgcgaaattt gcgaacgtgc agccccacag
     2461 cggggcctcg gccaacctgg cggcattcta cgcagtcctg cagcccggtg atacgtacat
     2521 gggcatggat ctctcgcacg gcgggcacct gacgcacggc agcccggtaa acttctccgg
     2581 gctctactat aaacctgtac cctacggcgt caaagaagac acccacctca tcgattacga
     2641 ccaggtcgaa cgccttgccc tcgagcataa acccaagatg attatcgcgg gtgcttcagc
     2701 ttatccgcgt gtcatcgact tcccgcgttt caggcagatc gccgataaag tgggcgccaa
     2761 agttatggtt gatatggcgc atatcgcggg ccttgtcgca gcgggggagc acccctcgcc
     2821 actcgagtac gccgatattg tgacaacgac cacacacaaa accctgcgcg gaccgcgcgg
     2881 tggcgtcatt ctgaccaaca gcgaagagat gatcaagatc ctcaattcac gcgtttttcc
     2941 cggtgtacag ggcggaccgc tgatgcacgt catcgccgcg aaagcggcgg ctttcgccga
     3001 agcgctgacc cctgagttca agacctacca aaagcaggta gttgctaacg cgcgcgcgct
     3061 ggctcagcgc ctggtcgagc gcaagttcag cctcgtctcg ggcggaacag ataaccacct
     3121 gatgatcgtg aatacgttcg ataccaaggg tatcacgggc aaagacgcac aggcgatgct
     3181 cgaactggcg aacatcacca cgaacaagaa catgctgccg tacgataaga ataaaccggc
     3241 ggttgcgagc ggtatccgcc tcggtagccc ggcgctgacg acgcgtggtt ttggggaaag
     3301 cgagttccgc gagacggctg atatcattgc cgatattctt gaagccaaag gctccgaggc
     3361 tgctatcgcg gatgcaaaga agcgcgtcgc tgcgctctgt gcggcatacc cgatggataa
     3421 attccgtctc ggctaaggaa gcctcgttgc cacagaagct cctacaggtt gtcatgctcg
     3481 cctgcctcgt gggcggtgct gcgctcgcag cagacacgcg gcgcaaggca attcgccgcg
     3541 aaatgattga aatggatatt gccgtgcgca acctgacgag taccatcgcc atggcggaca
     3601 ggaaggtgct cgatgattct ctgcagcggc tcgtaacctg gcagatcaaa gaccaccccg
     3661 acctgggcgc tgcgtttcgg caggtgctcg ctgactggca gtccaaaaat ctgagccgtt
     3721 acggcactga actgcagaaa gaagcacagg ccctgcgcag ttttgctgcg ggcagggcag
     3781 gacgcctgtc tgattcagat tgggctaaaa tcggctcagg cctcaaccgc atactcatca
     3841 attgccagaa ctgccacgag gccgcgcgta aggagccgaa ataaatgaaa aaattctacg
     3901 agcgttactt cagcatacac aactggctgc gggaaaatcg cgtggtgggc agcctggtat
     3961 cgctcggtgg gcttgtggcg tttgtttttg ccatcaaaac cagcgtgctc gatgcgaaca
     4021 atattccatc gccttcgatg gagcctacac tcatgatcgg agacttcctg tttgtcaaca
     4081 agatgcgcta cacgatcgac ctgccataca ccaatatcca ccttgtgcgc attgcgtacc
     4141 ccgagcgcgg cgacatcgtg actttcaccc caccacagga ttccgcatcc tatgcaaata
     4201 agaccctggt gaagcgcgtg gtgggagttc caggcgatac ggtcgaagtc gaagaccatg
     4261 aaatcaccat ttccggcaag aaatacccga ccacgccggc aaataacgaa gagctgctca
     4321 agacgctcgc agatggaaac accggcgaac gcgagcggct gtatgaagaa aaaattatcg
     4381 atcccaaaac gggccggctg gtggttgcac actatatgat gaaatcgcgt atccgggcga
     4441 cggatatgct gatggcgacc ccaggccgaa agtgggttat ccctgccaaa aaatacctca
     4501 tgatgggtga caaccgtgac cacagctctg acggccgtgc ctgcgaaatg gtggaagcca
     4561 aataccgcaa ggcctgcaac gatttcaaca actgcatgta cgatacctgg tcgggccaac
     4621 gcaccgaaaa acagtgccag gccatttacg ccggggcaaa gggcgaaacc tgggggctca
     4681 tcgacgcgga caatatccac ggcaaagtat acctctcgta tctttcagtg aattggggta
     4741 caggtatgaa cagcgagctg aacccgattt ttaacctctg gtatacgatt acgggtaagt
     4801 tcagcggagt ttacgttcgc tgggagagaa ttttcagaag gatatactga ccgccgataa
     4861 taaagaaggc atgcggcgga cactagtttc atctttatgt ctcattgtag ccggtgctga
     4921 tcttgttgca cagccgtatt taagcagccg catcgtctct gtccgctctg ccgaaaccgg
     4981 gcgcattgaa attaccgtcc ggtcggaaaa aagcggggag tttgatgcta ttactcccga
     5041 caatgcgccg cagtttacgg taaccgaaaa cggtcgcgcg cagtcttcgg tgcaatcaga
     5101 gctgctgccg cagcacagcc aggtatcacg cacggtgatt ctcgccgacc tcagccattc
     5161 gctcacacgc cggcagtttg cggattttaa gcgcgctgcg acagattttg tcggccggct
     5221 gaagcccggt gatctcgtgg cgcttgtcac tttccaccgc cgcgtctacc gcgagctcaa
     5281 ttttaccggc gatagggagc tcctgaagaa gcgtgttgca gcgctgagac agacgggcag
     5341 gcgcaccatg ctttacgatg cgcttatcga agcgcacaag atgctgcgcg atgtacctga
     5401 ccgcaaggcg attgtcgtct acaccgatgg taaggagaac gcctcgcgcg tacgctttga
     5461 cgacctgacc gaagttttta cctcgcagcc cgtgccgctc tttgttgcgg gcaataagaa
     5521 atccttttca ctccgcaagc ttatccggct ggcgcgggtt tcgggtggcg atgcgttcag
     5581 ggcagaggac cggcacgacc tgggcaaggt gttcagttac ctgagccgtt tgcgcgcgca
     5641 ggaattccgt atcagctact tgacgcagca gaagcccggc agcgccatcg acctcgaagt
     5701 ccgctctccg cagtgggcac aggctctcag tgcatcgtac accattccgc acggggtgct
     5761 ggcgcaagcg ccggttcggg aaaagctgtt ttcgtcggct ctgcctgaaa cgatcagggc
     5821 acacatgcct gagatactgc tatcgctgat tgtcctgctg cttatcgctg tcattatcat
     5881 gctgtttttt cgccggcagg aaatcagcgt caaggtagag aacgtgcaac caccggtatt
     5941 cgtgggtaac gatgaaatac tgccgccagg tctcaagcgg caggaaactc cccgcgcaaa
     6001 attgccgctc gactaccacc acggctggct ggtcgagaaa gaagggccgc atacggggcg
     6061 caagtaccgc atcaactggc atacggtgac gattggtttt gccgacgatt gttcgatcgt
     6121 gatcgacgac aatacggtct cggctaaaca cgcaaagatc gaacggcagg gcaataaatt
     6181 tgtactctac gatttgcttt cagagaatgg cacctggctg aacggcaaga aacttttgcg
     6241 acctaaagaa ctcaatgatt ttgatgaaat tggcatcggt cgcacaaagc tgattttccg
     6301 caagagcgca ggaaagaatg aagaaagaca cggcgaaaaa gcgtaagaca accccggtaa
     6361 caaaaaaaaa tccctattcg attgagctga gaaagctcag gctcggcaag agcgaagtga
     6421 ttctgctggg tacggctcac gtcagccgcg agagtgtcgc cgatgtcgag cgcgccatcg
     6481 tcaaagaaaa gcctgacgca gtcctgatag agctcgatga gggcagggcg cgcaacctgc
     6541 gcgaccccga ccactggaaa cataccgata tcgtccaggt tatcaagtcc ggtaaaatat
     6601 acctgctctt ttcatcgatc ctgctttcca ttttccagaa aaagatgggt gaccggctaa
     6661 cctccgcccc cggcgccgaa ttcaagaaag ccatcgagct ttcggagcaa aagaaactag
     6721 cgcactattt tgtcgaccgc gagatacgga taacgctgaa acgtgcgtgg caaagcgttg
     6781 gattcttcgg caaattacgg ctgatgagcg agctcatcgc ttcgctcttt gtcaccgaag
     6841 atactgccag cgaagatatt gaaaagctta aagagaaaga cgcgctacag actctgctcg
     6901 attcgatgcc accttctttc cggcgcattc gcgagattgt gatcgatgaa cgcgattatt
     6961 acatggcgca aaaagtgcgg gatattgtcc tgcacgcaga aaagaagccg aagcgcgcgc
     7021 tggttgtgat cggcgcaggt cacctgcgcg gcatcgaaga ggccatgcag cgcgatcacg
     7081 atatgcaggc cctgacagct gtcaaagaag gcagcctgtg gaaatcgctg gtggcattcc
     7141 ttgctccgat tgcgatcatt gtggccttcc tctcgtattt cactttttcc ggcggtaaga
     7201 aagttgatat tgccgaaaca ctgaagatct gggtgattat taagtgttcg gtgactgcgg
     7261 ccatcacgat tatctggcgg ccgcatattc ttgcctacct cgcaggtatc ctcgtctcgc
     7321 ccgtcagtac gtttctgccg gtcattaaag tgggctgggt ttcggccttg ctcgaggcaa
     7381 tgctgcgcaa accagaagtt acggattttg aagagatgcc cgaggcgacg accaggttcc
     7441 gcgctttcta tcgtaaccgc atatttcgga tctttatggt tttctttctc ggtcagtttt
     7501 cgagcgcgct cggctactgg gttttctttt ggtacgtctg agcaagcgcg tcgaccaggg
     7561 cgccaatcag gccgccaaag ctgccgttgg tcatgaagca gacaatatcg cccttctgta
     7621 gttgtgccct gacggtatcg acccggtttt ctgcaggtag caaaaaggcg gagcgtcccc
     7681 gttcattaag tgatttgacc agcagcgcgg tatcaagccg ttcttcgggc ctgtactttt
     7741 cggggcggta aatttgatcg atgaacgcta cgtcggcgtt tgccagtgca tcggtaagtt
     7801 ctgactggaa aatattgcga accatcgtat tactgcgcgg ttcgatgagc acgacgagcc
     7861 ggcgtgccgg gtagatttcc cgcagggtct gcaggttagc ggcaattgcc gtgggatggt
     7921 gagcaaaatc ttcgatcacc gtaacttcgg cagtttcaag tctcagctcg agacggcgct
     7981 tcacgccgtg aaagctgagc attgcctgtt tgaactgttc acgactcaca ccgactgatt
     8041 cgagcagcgc gaggcaggcg agggcgttca gtgcattgtg ccgtccgaac agcggacttt
     8101 cgaactcaaa tatttccccc cgcacgcgcg acttgatggt tgtagtttta ccggtctgca
     8161 attcgagtag ctggtaatct gccgcagcag atgcgccaaa ggtgatcaca ggagcgaacg
     8221 agctgcgtga cagctgcagg cacgaagcat tgtcggcatt cgtgacgagc acaccgtttg
     8281 ccggtatgat cttgatcagc cgggcaaatg tccgttcaac gtcacgcagg tcggcaaaaa
     8341 tgtcgccgtg gtcaaattca atattgttga gaatgacatg cgtcggccgg taggaaagaa
     8401 atttggagcg ctggtcaaaa atcgaggtgt catattcatc gccttcgatg acaaacaggt
     8461 cggccgaacc cacggccgca ctgcgcggaa aatctgcagc cacgccccca atcatgtagc
     8521 ccgtatcgat tgccgcctcg cgcaaaacgt gtgcagcgag cgaagtggtt gtggtcttgc
     8581 cgtgggtgcc cgcgatgacc agcgagcggc ggcggccgat aacatactct gcgacaaact
     8641 gcggcaggga aagtatttcg aagcgctgcg cgagcgcata ttcgagctcg acattgccac
     8701 gcgaaatcgc gttaccgagt acgacaaggt caggcccata ctcaagattc tgctctgcgt
     8761 aaccttcggt gtaaggtatt cccgaggcct tgagttccgt tgaagtggga gggtagaggt
     8821 tctgatcaga gccgcgaaca gaaaagccga gctccaccgc catctgcgcg gcccctgcca
     8881 tcgcggtgcc gccaatgcca ataaagtgta cggttctgcc cgcaatttgc cgcgccatgc
     8941 gccatgacct tagcctaaat ggctcgtgcg gtgaaaaact cttgctcgaa cgtatagggc
     9001 aggcacaccc gacgcatgag ccaaaaaagc agggccgaag agatcgcccg ggtcaccgat
     9061 acaaacggca tcaaggtacc ccgaattcgc ctctcgatgc ggtggagcat tgtatccctg
     9121 ctgacgctgc tgattatctt ctccgcgggc ctgatcattc tcgtctccta catcggtggc
     9181 aggcagtcta cgcagtacct gatggatacc ctgatggagg ataaggccta ccgctttatc
     9241 gacaagacct cacaattttt actgcaggcg cacaaccttg cggaagatgc acgcgaagaa
     9301 atcgcaaagc tcaataccct gacgcagcgc cgcgagctcg aagaagagct gcgcgatatt
     9361 tttgaaagcc agccctacta ttcagccgcc tactgggtac agtctggcag agcggtcgtc
     9421 gctcgccacc aggcaatacc cgaagcgcag gcaacaaacc tgatggatga actggacgat
     9481 gagaagcata atgccgccgc gcatctgaag gatctcactt tcgaggacaa gcaggttcca
     9541 gccggcagtg aatttccctg gcagaagaag gagtatgccg acggtaagaa gatgatgccg
     9601 ctgcaccggg tattctcgca gaatgcacca ggttttactt tttgttcacc ggtaaacccg
     9661 gcacggggta tcagtgcggg ggctgtctgc gtcgatatca cgctgggtga cctggcacta
     9721 tatatcggct ccgtcaaggt aggcgagacg ggcagggcgt tcattgcgga taagaacctg
     9781 aatgtactcg cagccccgca gcaggaccgc acgagttcag cataccaggt gcgacaggac
     9841 agtacagagc tgaaaaacct cacggtattc ggcaatgcag agtttaatgc agcttatctt
     9901 gcgatgaaca agaaactgaa gaagttgcag aagaacaagc gctggctcaa gacgcgcaaa
     9961 cccttctact ttgattttaa gcacggtggt agccgctacg tgggtatctt cacgcagttt
    10021 ccccgctaca ccgggcttga ctggcaggcg ggtgtcatta ttcccgaaga tgaattcttc
    10081 tacttcgtgc gccggaatac gcgcatcgtc atagcggcct cgaccctttt ggtgctgctc
    10141 gcagtcgtgc tcggctatat attttcgcgc cggctttccc ggccactcaa cgaactggcg
    10201 catgaaatgg acaagataca gaattttgat ctgacctcgg gcaaagctat cgtatcttcg
    10261 attaccgacg tcgacaacat ggtcatgtca ttctacaaga tgaggcaggg acttcgttcg
    10321 ttcggtaagt ttgttcccgc tgacatcgtg cggcagctca ttgcacagga aggtgacgcc
    10381 cacctgcagg gggaaaagcg ccagctgacc gtgcactttt ctgacattga gggatttacc
    10441 acggtatcag aaagtattcc gccggagcag cttgtcgagt tgctcgctga gtacctcggt
    10501 gaaatgagcc gcattattgc tgaagagaat ggcacggtcg acaagtatat cggggacgcc
    10561 gtgatggcat tctggggagc tccgcaggcc gttgaaaacc atgcgcactg tgcagccagg
    10621 gcttccctgc gctgccagaa gcggctcgcg gagctggaga acaaatggca ggcagaaggc
    10681 aaaccggttt ttcgcgcccg cattggtctg aataccggtg agattatcgt ggggaatatg
    10741 gggtcggccg aacggctcaa ctataccgtt atcggcgacg ccgtgaatct tgccagccgg
    10801 ctcgaaggca tcaacaagta ttacggcacg cggatcatcg tcggcgaaac gacgcagaag
    10861 ctcatcgccg atctctatgc cacacgtttg ctcgattttg ttgccgtcaa aggtaagagc
    10921 gaggccatac gtatatatga acttgtcgga gaaaagcacg ccctggacgg tacttctgcc
    10981 agcttcatcg cgcattacga agcggccatc ggtcactacc ggaaccagca atgggatgcc
    11041 gcagagaaag aattcagggc agcgctggca gtgcgtccgg acgaggccgc agagatgatg
    11101 ctcggccgca tcgaagaatt tcgccgcgaa agcccgcccg ctggctggaa cggggaatac
    11161 gtgatgagca gcaagtagag tgcctggctt ttctgacgaa tcactccatt gccttgccct
    11221 gcatttggca gggcgtacgc ttagtgaaaa gaaccgccgg ctactcgaag ccgcaggctc
    11281 actgctgcgc ctggccccgg tattgcttgc caatgagttt gcgcagttcc ttggacaggc
    11341 aagagaaacg ctccaccggc ttggtactga tactgttgtg actctttctg atgacgatta
    11401 ccccccgttg cttgccgaaa tcagcacagc cccctttgcc ctgtttctgc gcggcaaccg
    11461 acgcctgatc ggagctgaga aaatctcaat tgtaggcacg cgcgagcctt caccgacggg
    11521 cagaatggca gcccgtaata tcgtggcaca ttttgccgcc caggaacggg taattgtttc
    11581 aggtatagcc cgcggcatcg atgccgtagc tcatcacgca gcactgcagg caggaggcgc
    11641 aaccatcgcg atcctgccga acggttttga tcacctgtac ccgctcgaga accgggatat
    11701 ctatgagctg gcacgcacta gtgacagagt ccttttgctg agcgagtatt cacctgctga
    11761 aaagccgcaa cgctaccagt tcgtgcgtcg taaccgaatt attgccggcc tgtcgtcgac
    11821 tacggtcgtg atcgaagctg gtatcaagag tggggcaatg atcaccgtta atcacgcgct
    11881 cgatgaaggc cgtgatgtcg cagcgctaag ccacatgagt cttcttaaca atgccggtgg
    11941 ggaaaaactt gtggccgacg gagcaat
//
LOCUS       DAOQJF010000058        11909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2687_length_11909_cov_5.610005, whole genome shotgun sequence.
ACCESSION   DAOQJF010000058 DAOQJF010000000
VERSION     DAOQJF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11909
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2687_length_11909_cov_5.610005"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3406)
                     /locus_tag="PLF85_04775"
     CDS             complement(<1..3406)
                     /locus_tag="PLF85_04775"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNE19005.1"
                     /translation="MIFPVTGFPVAHQVQLKLPGVVFHDGRIRLAGFADGAGLISHAA
                     PSVSELFVAKAAWLSDLLYRILARTDFGVQMQRQTQQQLANYLGVTEDKADLALCGLL
                     WLSAANPVLAPLRACFVHIADIAHLDFLTSSPAGRRLLDLLLHSRASLDAQLEALLTT
                     FPDVLTEAERRLFLQVLYYERTAQAAGGVGPGEPHYGYALATGSALNRSNDREWMQSL
                     VLVAKIVPVWLADIRRRYGIEAWRLDQVPAAELEHLRSLGINGIWLVGLWQRSAASAR
                     IKSLTGGKNYASAYSVFDYRVADEWGGEEALARFHELCARHGVRLGCDMVPNHTGIDS
                     RWVREHPEWFMQAEFSPFPNYRFDGENLSHDPATQIHIEQGYYSRSDAAVVFALERDG
                     RRRYIYHGNDGTGLPWNDTAQLDHTRADVRQAIIETALSLSRYFSVIRFDAAMTLTRD
                     HYRRLWFPAPGEAPVIASRSAYARPQHEFDQRMPREFWLELVEAFRERSTDTLLIAEA
                     FWMTEMYFVRSLGMHRVYNSAFMHALRDEQNGEFMRTLREFREHDHATLMRFVNYLST
                     PDEESAQAHFPHADKYFGAMVLMATLPGLPMLAPGQTEGYREKYGMDQDAPLRGESVD
                     EYLVHRHRAQISPLLQRRSEFADPLSLEFPAAEKPEQVILYTTGRSQRHIVIFNNSPH
                     PANVRLHDFTGFFPPGCTALGWKDLLSGHKYRVEKNSLNDRALIVELEPYASRVFSEF
                     AEAASEQAVQLRRYPQHGEYLAGVAVPVTALRSERSCGIGEFSDLKLLVDWCCESGQK
                     IINLLPVNDTGYNSSPYSALSAMALHPVYLCLDELPLTPALRQEVNDMRRQSAENVRV
                     HFYEIVRFKEHILRTLYDADPEAARLLVPAEFASQQWLLDYALFRVLKELFGGSAWFD
                     WQAASTPPEIAALVAAPTPAARAEFRSRHQHLVDYYIWVQYLLDQQLRQVRDYANGRG
                     VFIKGDMPIMLERDSVDVWLNPDLFNHHLRAGAPPDMFSPDGQNWGFPVYNWPAHERE
                     NFSWWRARIAQLDRYFAAYRIDHVLGFFRIWSIAQGNRSGLDGFFLPSHAVTAEQLNT
                     LAAGEQNVACLTRVPVYPDEMGALPNDTQAVLSHYEGVAFLPDEYST"
     gene            complement(3413..5806)
                     /locus_tag="PLF85_04780"
     CDS             complement(3413..5806)
                     /locus_tag="PLF85_04780"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNE19006.1"
                     /translation="MLPLSADLKAELQHGFHRHLNYSLGRSLDELSPEDAYISLSLSL
                     RDLMMHRLIVKRHRIRHKKRVYYLSMEFLMGKMLKTALVNMNLSAIAAEMLGQYEIRL
                     EDLYEIEPDAALGNGGLGRLAACFLDSMAHLDLACIGTGLCYEFGLFKQSFRDGAQQE
                     SPDIWLAGNYPWLIDRHDKRYEIRFYGHVHRDGDKVTWHDGEVVMARARDMLIPGNGS
                     EGIALLRLWWAEHSADFGLDYFQHGDYVKAMENQIYSESISKVLYPNDNNLRGQELRL
                     KQEYFLSSATIQDAIAAFKHEEADLSLLPERAFFQLNDTHPAIAIAELMRILIDREGV
                     SFEKAWEMTRKCTGYTNHTIMPEALEKWNLDLFGRVLPRHLEIIYDINFRFLAELRDR
                     GFDHEAIARASIIEEGHPKRIRMAALSVVGSSAVNGVSALHSELVKKNLFPEYARLWP
                     EKFSNKTNGIAFRRWLVAANPGLTALIEETIGRDWYADHSLLSKLLPYAQDAGFRSSW
                     QQVRDHNKKLLYAIIKERNHITLPPESFLDVHVKRIHEYKRQLLNLLRIGRICLDIMA
                     GTYKIETHRTFIFAGKAAPGYHRAKLMIRLANALSAYIEKNPLIRKRIQVVFLPNFSV
                     SLAERIYPAADLSEQISTAGTEASGTGNMKFMLNGVVTFGTLDGANIEIGEEVGKENI
                     YIFGHTFEELNKLQLDGYHPVALAERREIREVLQFLEMLHGDFREFSDALTYQGDGYF
                     HLADFDSYWQVQNQIMADYDKRDNWIIKGINNTARSGKFSSDRTVLQYAKETWKIIE"
     gene            complement(5869..8604)
                     /locus_tag="PLF85_04785"
     CDS             complement(5869..8604)
                     /locus_tag="PLF85_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNE19007.1"
                     /translation="MLTAQWHLGSVGAQAQRLQADSVHGLTAAEAALRLKDHGPNRLT
                     TRSRRSLLELLVEQFRNLLVVLLLLASILSFFLGSFNDGIVLLTIVVINALVGFFQDW
                     KSENIVSRLGSLVTDTAIAVREGRRIEVPTTSLVPGDLVWLQEGEGVPADCRIVSGES
                     IMANEMALTGESVAAEKEASYTTESDVPLPERRNSLFFGTTLARGEVLCLVCATGDNT
                     ELGKIAVSSQSMDREASPLQKELNILGQHITRFALSLAAILFVVQVWRDEPIKTALIF
                     AVSVAAAMVPEGLPAQISMGLSLGVARLARKNAVVKRLSSVEALGAATVIASDKTGTI
                     TRNEMTITSAFFEGLNYRITGHGYNPKGEIFDESGQIMSSGNLGERKILFLAGFLSST
                     GTVNPPDEFHQDFYALGDPTEAAFATLLMKAGFEPAVIAADYPRLRLFPFDSERKRIS
                     IVREHNGRRISFVKGSIESLLPLCNRHRVQGKEEELTAKEREELLAVSRAYSAQSLRV
                     IALAYRDLPDGEDISQQAAERDLVYSGFVTMVDPPRAEVKTAVQAAHSAGIRTVMITG
                     DHAATARSIAIEIGMEHDLDRPIEVINRDSLKAMDAERLTEALAQRSVIFSRVSPDEK
                     LRIIETLQKQGDVVAVTGDGVNDTLSLKRADIGVAMGVGGSKVAQEAASLVLLDNSFA
                     TIVSAIREGRTIFRNLQTNVVATLSSNMTELLCVLAGFALIPFGIPPIILPVQILLVD
                     LVGEMLPLLMLTYDPAMPGVMQLKPRKKGALLDKARLLTIGVTGVVRGLISTAVFIAV
                     FRAHTGEAGAWETGLSATFVTIVMTQFIGIFYLRGTGRLLSAELFSNLWLFVGIGLSA
                     LAMIAIVYVPALNIYLHTRPLAAGDIQVVALGLAVFAVFSSAYRLLSGRLQAHSG"
     gene            8755..9891
                     /locus_tag="PLF85_04790"
     CDS             8755..9891
                     /locus_tag="PLF85_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19008.1"
                     /translation="MGKAREDAWYLSLGAGENQLPLILAAKAAGLKVVGVDRNIEAVG
                     FRHCDLKIEESILNYRKIFYKLSSLVEPGQIAGGYAASYGEALASFAFLCERLSIVGL
                     SRTLIETVLDKLQVRRRLAGLEHQNFAQPLFMEILPSMHREQIEQLGFPLIAKTRRGA
                     SKRHVYLLHNWFEVKNFLSRRNLEALGVRGPDFLFEQFIEGDEIIVCGFAQNFHYNLV
                     SVHDRIPAQQPPYIDLEHRFPSRYAHLAPQIQEIHSQVCMRLQLSDTPVVSEWKVVAD
                     RLYLVELSAQIPGEHVADFLIPRGLGYDYFGNLLRLTLGEKIEPVPEKVRQPITVRYW
                     AENPGFGEWQELTRKAAFARVINNNPPKTISSNLDRFGVAGFVG"
     gene            complement(9910..10587)
                     /gene="pdxH"
                     /locus_tag="PLF85_04795"
     CDS             complement(9910..10587)
                     /gene="pdxH"
                     /locus_tag="PLF85_04795"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNE19009.1"
                     /translation="MFFSADGLYSKSERAVMNLGAVRSEYKGDSIKDLPPDPHKGAQL
                     WLEQALKTEAEATAFSLATHDAEGELSVRVVLAKSITEAGVTFYTNGLSRKGRQLAAN
                     AVAAGVFFWPASHRQLRFEGKVTRVSDAEADEYFASRPHESQVASSISQQSTPLDAYE
                     SLVKRFEAARADYAGKKVPRPPHWGGYQIAFEKVEFWQGQPSRLHQRLLYTQKPDGTY
                     DRTWLEP"
     gene            10593..11198
                     /locus_tag="PLF85_04800"
     CDS             10593..11198
                     /locus_tag="PLF85_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE19010.1"
                     /translation="MAKPAAGQPIGKRDQTKILNRQQILEAAKTLFAEKGFEATNVRD
                     IIHKSNLSPGTFYNYFQSKEEIFEVLTDEIIDEVRSQIQDSYKNVKMDRAEINKSLKK
                     FFQIFLGNPRLMKFLSRNQSYLRELRSRGRFDGMLSDLEKALEDGVKNGMIPQVPVKI
                     VSIAIFGAVFEIIATMVSTPNFEVGKAIETLSQLLFIQTGK"
     gene            complement(11205..>11909)
                     /locus_tag="PLF85_04805"
     CDS             complement(11205..>11909)
                     /locus_tag="PLF85_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE19011.1"
                     /translation="RPDTPVPMAAQAAEERQAQWKRHLQFLTGYQDEFELVTSTNAFA
                     TEDMDLAKADLSQRSDIALGKTILSLRKNELAAAHGAHLPNIYLDGSYRPALEENQRT
                     SYFGGIVAELPLFQGGQLLENQKIAQSRLRQAELELSLAERKARTELADARESLVKAS
                     AEKAAYAESAKAAEKNYLAQLSDMRLSLATILDVVQSMEDLQAARIARAQAEYREAIA
                     QVRFFVATGRLFPAKQ"
BASE COUNT         2604 a         3478 c         3386 g         2441 t
ORIGIN      
        1 cggtgctgta ctcgtcaggc agaaatgcca cgccctcgta gtgactgagg acagcctggg
       61 tatcattggg cagcgcgccc atctcgtccg ggtaaaccgg aacacgcgtt aggcacgcca
      121 cgttctgctc accagcagcg agcgtgttca gctgctcagc ggtgaccgca tgcgatggca
      181 gaaaaaatcc gtcgagaccg ctgcggttac cctgtgcgat ggaccagatg cgaaaaaagc
      241 caagcacgtg atcgatgcgg taggcagcga aatagcggtc gagctgtgcg atacgcgcac
      301 gccaccagga aaaattctcc ctttcatgcg ccggccagtt ataaaccggg aacccccagt
      361 tttgcccgtc gggcgagaac atgtcaggcg gcgcaccggc gcgcaggtgg tggttaaaca
      421 ggtcagggtt cagccagaca tcgacgctgt cacgttcgag catgatcggc atgtcgccct
      481 tgataaaaac gccgcgaccg ttggcatagt cgcgcacctg ccgcagttgc tggtcgagca
      541 ggtactgaac ccagatgtaa taatcgacga ggtgctggtg cctgctcctg aattcggcgc
      601 gggctgccgg cgtcggcgcc gcaaccagtg cggcaatctc cggtggagtg ctcgctgcct
      661 gccagtcaaa ccacgccgaa ccgccgaaga gctctttcag cacacgaaac agcgcgtagt
      721 cgagcagcca ctgttgcgag gcaaactctg ccggcaccaa aagccgcgcg gcttcgggat
      781 cggcgtcgta gagcgtacgc aggatatgct ctttgaaacg gacaatttca taaaagtgga
      841 cccgcacatt ctctgccgac tgccggcgca tgtcgttgac ttcctgcctg agtgccggtg
      901 tgagcggtag ttcatcgaga cagagataga ccgggtgcaa tgccatggcg ctgagcgccg
      961 aataaggact gctgttgtag ccggtatcat ttaccggcaa aaggttgata atcttctgcc
     1021 cgctctcaca gcaccagtca accagcagct tcaggtcaga aaactcgccg atgccgcagc
     1081 tgcgttcgct gcgcaaggct gtaaccggca ccgcgacccc ggcgaggtat tcgccatgct
     1141 gtggatagcg gcgcaactgc actgcctgct ctgaggccgc ctcggcgaat tcgctgaaca
     1201 ccctgctggc ataaggttcg agctcgacta taagagcgcg atcgttgagg gagttttttt
     1261 cgacacggta cttgtgccca ctgagcagat ctttccagcc gagcgctgtg cagccgggtg
     1321 gaaaaaaacc ggtaaagtca tgcaggcgca cgttcgccgg gtgcggcgaa ttattgaaaa
     1381 tgacgatgtg gcgctgcgaa cgccccgtgg tgtagagaat aacctgttcg ggtttttccg
     1441 cagcgggaaa ttcaagcgac agtggatcgg caaactcgct gcgtctctgt aacagggggg
     1501 aaatctgcgc gcggtggcga tgcacgagat actcatcgac actctcaccg cgcaaggggg
     1561 catcctggtc cataccgtat ttctcacggt aaccttcggt ctggccgggc gccagcatgg
     1621 gcaagccggg caatgtcgcc atcagcacca ttgcgccaaa gtatttgtct gcgtgcggaa
     1681 agtgtgcctg cgccgattct tcatcgggcg tgctgaggta gttgacgaag cgcatgagcg
     1741 tggcgtggtc gtgctcgcgg aattcgcgca gggtacgcat gaactccccg ttctgctcat
     1801 cgcgcagtgc gtgcatgaag gcgctgttgt agacccggtg catgccgaga ctgcggacaa
     1861 aatacatctc agtcatccag aatgcctcgg caatgagcag ggtatcagtg ctgcgctcgc
     1921 gaaacgcttc gacgagttcg agccagaatt cacgcggcat acgctggtca aattcgtgct
     1981 gcggccgcgc gtaagcagag cggctggcaa ttaccggggc ttcgccgggc gcaggaaacc
     2041 acaggcgccg gtaatggtcg cgcgtcagcg tcatcgccgc atcaaagcgt atcaccgaaa
     2101 aatagcggga aagcgacagc gccgtttcaa tgatcgcctg ccgcacatcc gcacgtgtgt
     2161 ggtcgagctg cgcggtgtcg ttccacggca gaccggtacc gtcgttacca tggtagatat
     2221 agcgacggcg gccatcgcgc tcgagcgcaa acaccaccgc ggcatcgctg cgtgaatagt
     2281 aaccctgctc gatatggatt tgcgtcgccg ggtcgtgcga aagattttca ccgtcgaacc
     2341 ggtagttggg aaaaggcgaa aactccgcct gcatgaacca ctcgggatgc tcgcgcaccc
     2401 agcgcgaatc tatacccgta tggttgggca ccatgtcgca gccgagccgc acgccgtgcc
     2461 gcgcgcagag ttcatgaaaa cgtgccagcg cctcttcgcc gccccactcg tctgcaacgc
     2521 ggtagtcaaa taccgagtag gccgaggcgt aattcttccc gcccgtcagg ctcttgatgc
     2581 gggccgaagc cgcactgcgc tgccacaggc cgacgagcca aattccatta atgccgaggc
     2641 tcctgaggtg ctcgagttct gccgcgggca cctggtctaa gcgccaggct tctatgccat
     2701 accgccgccg aatgtcggca agccagacgg gaacaatctt cgccacgagc accagcgact
     2761 gcatccattc acggtcgttg ctgcggttga gcgcgctacc ggtcgcaagc gcgtaaccgt
     2821 agtgcggttc gccggggcct acccccccgg ctgcctgtgc tgtgcgctca tagtagagta
     2881 cctgcaggaa aagccggcgt tcggcctctg tgagtacatc gggaaatgtg gtcagcagcg
     2941 cttcaagctg ggcatcgagc gaggcccggc tgtgcaacag cagatcgagc aggcgccttc
     3001 ccgccgggct ggaggtaaga aaatcgaggt gcgctatatc tgcaatgtgc acgaagcacg
     3061 cgcgcagtgg tgcgagcacc gggtttgccg ccgacagcca gagcaggcca cacagcgcga
     3121 gatcagcttt atcttctgta acgccgaggt aatttgccag ctgctgctgc gtctggcgct
     3181 gcatctgcac cccaaaatcg gtgcgtgcca gaatgcggta aagcaaatcg ctcagccatg
     3241 ctgccttcgc gacaaaaagc tcagatacag agggagctgc atggctgatg agaccggcgc
     3301 cgtctgcgaa acccgccagc ctgattctgc catcatgaaa tacgacgcca ggaagtttca
     3361 gctgcacctg gtgggcgaca ggaaaacccg tgacaggaaa gatcatgggg tactactcga
     3421 tgattttcca ggtctctttg gcgtattgca gcacggtgcg gtcagaagag aatttgcctg
     3481 aacgtgcagt gttattgata cccttgatga tccagttgtc gcgcttatcg taatcggcca
     3541 taatctggtt ctgtacctgc cagtagctgt cgaaatctgc gaggtggaaa tagccgtcgc
     3601 cctggtatgt cagcgcgtcg ctgaactcgc gaaagtcacc atgcagcatt tcgagaaact
     3661 gcagcacttc gcgaatttcg cggcgttctg ccagggcgac agggtggtat ccgtcgagct
     3721 gcaacttgtt taactcttcg aacgtgtggc cgaaaatgta gatgttctct ttaccgactt
     3781 cttcgccgat ttcgatattg gcgccatcaa gcgtaccaaa ggtcaccacg ccgttgagca
     3841 tgaacttcat gttgcctgtt cctgaagcct ctgtacccgc ggtactgatc tgctccgaaa
     3901 ggtcggccgc agggtagata cgctctgcca gcgaaaccga gaagttgggt agaaatacca
     3961 cctggatacg cttacgaatc agcgggttct tttcgatgta ggctgagagc gcgttggcaa
     4021 gacgaatcat cagcttcgca cggtggtaac ccggcgctgc cttaccggca aaaataaaag
     4081 tacgatgggt ttcgatcttg tacgtgcccg ccatgatatc aagacaaata cggccgatgc
     4141 gcaacaggtt gagtaactgg cgcttgtact catggatgcg cttcacatgc acatcgagaa
     4201 aagactcggg cggcagggta atatggttgc gttccttgat aatcgcatag agcagttttt
     4261 tattgtgatc gcgcacctgc tgccacgaac tgcggaagcc ggcgtcctgc gcgtacggca
     4321 aaagtttgct gagcaggctg tggtcggcgt accagtcgcg accgatggtc tcttcgatga
     4381 gcgctgttaa acccgggtta gctgcgacca gccagcgccg gaaggctatg ccattcgtct
     4441 tgttcgagaa cttttcaggc cacaaccgcg cgtattcagg aaacaggttc ttcttcacga
     4501 gctcgctatg taatgcagag acgccgttga cggccgatga accaacaacc gacaacgctg
     4561 ccatgcgtat gcgcttggga tgtccctctt cgatgatcga ggctcgcgcg atcgcctcgt
     4621 gatcgaagcc ccggtcgcgc agctcagcca ggaaacggaa gttgatatcg tagatgattt
     4681 ccaggtgtcg gggcagtaca cggccaaaaa gatcgagatt ccatttttcc agcgcctcgg
     4741 gcatgatcgt gtggttggta tagcccgtgc acttgcgcgt catctcccag gctttttcaa
     4801 aactgacgcc ctcgcgatcg atcagaatgc gcatcagttc agcaatggcg atcgcgggat
     4861 gcgtatcgtt cagctggaaa aatgcccgct cgggcaacag cgataaatcc gcctcttcgt
     4921 gtttaaaggc tgcaatcgca tcttgtatcg tagcactgct gagaaaatac tcctgcttga
     4981 gcctgagctc ctgcccgcgc aggttattgt cgttggggta cagtaccttc gagatcgatt
     5041 cagaatagat ctggttctcc atggccttga catagtcgcc gtgctggaaa tagtcgaggc
     5101 caaaatcggc gctgtgttct gcccaccaga gccggagcag cgcgatgccc tctgagccgt
     5161 taccgggaat cagcatatcg cgcgcacgcg ccatgaccac ttcaccgtcg tgccaggtga
     5221 ccttgtcgcc gtcgcggtgt acatgaccat agaaacggat ttcatagcgc ttatcgtgcc
     5281 ggtcaatcag ccagggataa tttcccgcga gccagatgtc gggtgactcc tgctgcgcgc
     5341 cgtcacgaaa cgactgctta aaaagaccaa actcgtagca gagccctgtc ccgatacagg
     5401 cgagatccag gtgagccata ctgtcgagaa agcaggccgc gagccggccc aggccgccgt
     5461 taccgagcgc ggcatcgggt tcaatttcgt acaggtcttc gaggcgaatc tcgtattgcc
     5521 cgagcatttc tgcggcgatg gcagataggt tcatgttgac gagcgccgtc ttgagcatct
     5581 tgcccatcag gaattccatc gacaggtaat agacacgctt cttgtggcgt atgcggtggc
     5641 gcttgacgat caggcggtgc atcatcaggt cgcgcaatga cagcgagagc gagatgtagg
     5701 cgtcttcggg gctcagctca tcaaggctgc ggccgagcga gtaattgagg tggcggtgaa
     5761 aaccgtgttg aagttcggct ttcaggtctg cggaaagcgg gagcaccgcc catgctgaaa
     5821 aagttctacg aaaaggccag tagttttgtc cggactaccc atccccgttc agcctgagtg
     5881 cgcctgcagg cgtccgctca gcaggcggta ggcagaggaa aaaactgcga agactgcgag
     5941 ccccagcgca acaacctgga tatcgcctgc cgccagcggc cgggtatgca ggtagatatt
     6001 gagggccggc acatagacga ttgctatcat ggcgagcgcc gaaagcccaa tacccacgaa
     6061 cagccagaga ttagaaaaca attctgcgct gagcaggcgg cccgtgccgc gcaggtagaa
     6121 gataccgata aactgcgtca tcacaatcgt cacaaacgtg gccgagagcc cggtttccca
     6181 ggcacccgct tcgcccgtgt gcgcgcgaaa gacggcgatg aacaccgcag tagaaatcag
     6241 cccgcgcacc acgcccgtca caccgatcgt taacaacctg gccttatcga gcaaagcccc
     6301 cttcttacgc ggctttagct gcatcacccc gggcatcgcc gggtcatagg tcagcatcag
     6361 cagcggcagc atttcgccca ccaggtcaac gagcagtatc tgcaccggta gaatgatcgg
     6421 tggaatacca aacgggatta gggcaaaacc cgcgagcaca cacagcagct cggtcatgtt
     6481 cgatgatagt gtagcgacca cgttcgtctg caggttgcgg aagattgtcc tgccttcgcg
     6541 tatcgccgaa actatcgtgg caaaactgtt gtcgagcagc acgagactcg ccgcctcttg
     6601 cgctaccttg ctgcctccaa cacccatcgc cacgccgata tccgcccgct tgagactgag
     6661 cgtatcgttc acgccgtcgc ccgttacggc gacaacgtca ccctgctttt gcagtgtctc
     6721 gataatccgc agcttctcgt cgggtgaaac acgcgaaaaa atgaccgaac gctgcgccag
     6781 ggcttctgtc aaccgctcag catccatcgc cttcagcgaa tcgcggttga tgacttcgat
     6841 cggtcggtcg aggtcatgtt ccatgccaat ctcgatggca atcgaacgcg ccgtcgcggc
     6901 atggtcgccg gtaatcatca ccgtacgaat tccggcgctg tgcgccgcct gcaccgctgt
     6961 ttttacttca gcgcgcgggg gatctaccat ggtgacgaaa ccagaataga caaggtcgcg
     7021 ctcggccgct tgctgcgaaa tatcttcacc gtcgggtaaa tcacggtagg cgagagcaat
     7081 gacgcgcaac gattgagccg agtaagctcg cgatacggcg agcaattctt cgcgctcttt
     7141 ggcggtcagc tcttcttctt taccctgcac gcggtgccgg ttgcagagcg gcagcaacga
     7201 ttcgatcgac cccttcacaa aagaaatacg ccggccgttg tgctcgcgca cgatcgagat
     7261 acgcttgcgc tcggagtcaa acggaaacag tcgcagcctc ggataatcgg cggcaatgac
     7321 tgccggctca aacccggcct tcatcagcag ggtggcaaac gccgcttccg tcggatcacc
     7381 caaggcataa aagtcctggt gaaattcatc gggcgggttg accgtacccg tcgaagagag
     7441 aaatcccgcc aaaaagagaa tcttgcgctc gccgaggttg ccggatgaca tgatttgccc
     7501 gctctcatcg aaaatttccc ctttaggatt atagccatga cccgtaattc ggtagttgag
     7561 tccttcaaag aatgccgagg tgatcgtcat ctcgttgcgc gtaatcgtcc cggttttgtc
     7621 cgaggcaatc accgtcgcgg cgccgagtgc ctcgacggaa gagagcctct tgacgactgc
     7681 attcttacgc gccaggcgtg cgacaccgag cgacagcccc atcgaaatct gtgcaggtag
     7741 accttccgga accatcgctg ccgcgacgct gacggcaaat atgagggcgg tcttgatcgg
     7801 ttcatcgcgc cagacctgca ccacgaacag gattgctgcc agcgacaagg caaagcgcgt
     7861 gatatgctgc ccaagtatgt tcagctcttt ctgcagtggc gaggcctcgc ggtccatgct
     7921 ctgcgaagag acggcgatct taccgagttc ggtgttgtca ccggttgcac acacgaggca
     7981 gagcacctcg ccacgcgcca gagtcgtccc aaagaaaagg gagttcctgc gctcgggcaa
     8041 tggcacatca ctctctgtgg tatagctggc ctctttttca gcggcaacag actcaccggt
     8101 cagcgccatc tcgttcgcca tgatactttc accgctgaca atacggcaat ccgccggtac
     8161 tccttcgccc tcctgcaacc agacaagatc gccgggtacc agcgaagtgg tgggtacttc
     8221 gattcgcctg ccttcgcgca cggcaattgc cgtgtcggtc acgagactac cgaggcgcga
     8281 aacaatattc tctgacttcc agtcctggaa aaagccgaca agcgcattga tcacgacgat
     8341 cgtcagcagc acgatgccgt cgttaaaaga cccgagaaaa aaagaaagaa tcgatgcaag
     8401 tagcagcaaa accaccagca ggttgcggaa ctgctccacg agcagttcaa gcagcgagcg
     8461 gcgcgagcgt gtggtcagcc ggttgggccc gtggtctttc agtcgcagcg cggcttcggc
     8521 ggcagtcagc ccatggacgc tatcagcctg caaacgctgc gcctgtgcac cgacagaacc
     8581 cagatgccat tgcgcagtca gcatggaata ccaataattt tgcctgatac cctacgccaa
     8641 gaaatgtcgc gccacgcggt ccaggtggca cctgaatggt ctcgggcaca gcacacaccg
     8701 tcacaaaatt ttttcgctac aaagataagc agactcacgc cgatactgaa aatagtgggc
     8761 aaagcgcgcg aagacgcctg gtatttatct cttggggctg gcgaaaacca gctgcccctg
     8821 attcttgctg cgaaggccgc agggctcaag gtggtcggcg tcgaccgcaa tatcgaagcg
     8881 gtgggttttc gccactgcga cctcaaaatt gaagagtcga ttctcaatta ccggaaaatt
     8941 ttttataaac tttcgagcct cgtcgaaccg ggacaaatcg ccggcggtta cgcggcgagc
     9001 tacggcgagg cgctggctag ttttgccttt ctctgcgaac ggctgagcat tgtcggctta
     9061 agccgcactc tgattgaaac ggtactcgat aagctgcagg tgcgtcggcg ccttgcaggg
     9121 cttgagcacc agaacttcgc acagccgctg ttcatggaaa ttctgccgag catgcaccgc
     9181 gagcagatcg aacaactggg atttccgctg attgccaaaa cacggcgcgg cgcttcgaag
     9241 agacacgtgt acctgctgca caactggttc gaggtgaaaa atttcctttc gcgccgcaac
     9301 ctcgaagcgc tcggcgtacg cggacccgac tttctgttcg aacagtttat cgaaggtgat
     9361 gaaataatcg tctgcggctt tgcgcagaat ttccactaca acctcgtgtc ggtgcatgat
     9421 cgcattccag cgcagcagcc gccatatatt gacctggagc accgtttccc ctcacgctac
     9481 gcgcacttgg cgcctcagat acaggaaatt cactcgcagg tctgcatgcg cctgcaactt
     9541 tcagacacgc ccgttgtcag cgaatggaag gtcgtcgccg accggctcta cctggtcgaa
     9601 ctgagcgcgc agattcccgg cgaacatgtc gccgatttcc tgataccgcg cggtttgggt
     9661 tatgactact tcggcaacct gctgcgcctg acgctcggtg aaaaaattga accggtgccg
     9721 gaaaaggtcc gccaaccgat caccgtgcgt tactgggctg aaaacccggg cttcggcgaa
     9781 tggcaggaac tcacccgcaa ggcggcgttt gcccgcgtca tcaacaacaa tccaccgaag
     9841 acaatttctt caaacctcga ccgctttggc gtcgcaggct ttgttggtta acaggctctt
     9901 tcgaatccgc taaggctcta gccacgtacg gtcataagta ccgtcaggct tctgcgtgta
     9961 gagcaacctc tggtgcagcc ggctcggttg cccctgccag aactccacct tttcaaatgc
    10021 aatctggtaa ccgccccagt gcgggggacg tggaactttc ttacctgcat agtctgcccg
    10081 cgctgcttca aagcgtttga ccaatgattc gtaggcatcg agcggtgtgc tctgctgcga
    10141 aatgctcgag gccacctgcg attcgtgcgg gcggctggca aagtactcgt cagcttcagc
    10201 gtcgctcacg cgggtgactt taccttcgaa gcgcagctgg cgatgcgatg cgggccagaa
    10261 gaatactccc gcggcaacag cgttcgctgc gagctgccgg ccctttcggc tgaggccgtt
    10321 ggtgtagaac gtgacgcctg cctcggtaat acttttcgcg agtacgacgc gcacggaaag
    10381 ctcgccttcg gcgtcgtgtg tcgcgagcga aaacgcggtt gcctcggctt cggtcttcag
    10441 cgcctgctcg agccagagct gtgcgccctt gtgcgggtct ggcggcaggt ctttgatgga
    10501 gtcacccttg tactcactgc gcacggcacc gaggttcatg actgcccgtt cacttttgct
    10561 atacaggccg tccgcagaaa aaaacatcgg gcatggcaaa accggccgcc ggccaaccta
    10621 tcggcaaacg cgatcaaact aaaattctca accggcagca gattctcgag gcggcgaaaa
    10681 ccctgttcgc tgaaaaaggc ttcgaagcca ccaacgtccg cgacatcatc cacaaaagta
    10741 atttgtcgcc gggcacgttt tacaactatt tccagagcaa agaagagatc tttgaagtac
    10801 tgaccgatga gattatcgac gaagttcgct cgcagataca ggacagttac aaaaatgtca
    10861 agatggaccg ggctgagatc aataagtcgc tgaaaaaatt cttccagata tttctcggca
    10921 acccgcgcct gatgaaattt ctctcacgca accagtcgta cctgcgcgaa ctgcgcagcc
    10981 gcgggcgttt cgacggtatg ctcagcgatc tcgaaaaagc gctcgaagac ggcgtcaaaa
    11041 acggcatgat tccgcaggta ccggtcaaga tcgtctccat tgcaatcttt ggcgcagtgt
    11101 tcgagataat cgccacgatg gtcagcactc ccaactttga agtcggcaaa gcgattgaaa
    11161 ctctgtcgca gctgcttttt atccaaaccg gcaaatagcc gcgcctactg cttcgccggg
    11221 aaaagccggc cggtcgcgac gaaaaagcgc acctgcgcaa tggcttcgcg gtactcggcc
    11281 tgcgcgcgcg cgatgcgtgc agcctgcaaa tcttccatcg actgcaccac gtcgagaatg
    11341 gtcgcaaggc tcagccgcat gtccgataac tgcgccaggt agttcttttc ggcggctttt
    11401 gccgattcag cgtatgccgc cttttctgcg ctcgccttca ccaggctttc gcgcgcgtca
    11461 gccagttcag tgcgcgcctt gcgctccgca aggctcagct ccagctcggc ctgcctcaat
    11521 cttgactgcg cgatcttctg gttctccagc agctgcccgc cctggaacag ggggagctcg
    11581 gccacaatac caccaaagta cgacgtgcgc tggttttctt cgagcgcagg acggtaagaa
    11641 ccatcgaggt aaatattcgg caggtgagcg ccgtgcgccg cggcgagttc attcttccgc
    11701 agcgaaagaa ttgtcttgcc gagtgcaatg tccgaacgct ggctgaggtc ggcctttgcc
    11761 agatccatat cttcggtcgc gaaggcgttc gtcgaagtca ccagctcgaa ctcatcctgg
    11821 tagccggtca gaaactgcag gtggcgcttc cactgcgcct gccgctcttc tgccgcttgg
    11881 gccgccatcg gcaccggcgt atcaggtcg
//
LOCUS       DAOQJF010000059        11864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2699_length_11864_cov_5.311711, whole genome shotgun sequence.
ACCESSION   DAOQJF010000059 DAOQJF010000000
VERSION     DAOQJF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_11864_cov_5.311711"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="PLF85_04810"
     CDS             <1..1774
                     /locus_tag="PLF85_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19012.1"
                     /translation="GYSLALIAWRWFHSRRRADRSIVIIVAALMLGAAFMLTNVPGTQ
                     GISFPPLGALQFIPAGMIAFAILRYRAIVIRGEATRIANRFQLMSLVGVGFLVFVYAR
                     TLPEGLETIKIWNHLSVIAASLLLGFSMLLFVLVRPVAAAMDDSFHLLDEQKKQLEES
                     EKNLREIADVSRSVYASVNLNGIIDLMFAYVQQRFGIEGLWLTFCDTAQNRIYHVRHN
                     ADTQLPRETLGFMEQFSVDLTPDAGLLYHTWQRKRITYMRRLACPDKMAELDRILAEK
                     LDLKGYVHVPLVLQNEVIAVLMGMNFGKKLHLSPKDLRSLELFAGQIAGALSTARLLK
                     EINQEKKKSDELLLNILPRGIADQLKERGEVTPQFYDSVTILFTDFVGFTRSALTMVP
                     TELIEQLNRIFYQFDAITRRYNLEKIKTIGDSYMAAGGLPEKNATHYLDVCLAALEIV
                     KMTEQVIAMSGGTSTESWQLRVGIHTGPVMAGVVGQYKFVYDIFGDAVNVASRMETTS
                     EAARINISADTYHKVKFFFDCEYRGRIHIRNRGEVEMYFLNGLKPRFLTKHGAPNAEF
                     REIYEKIRNGARIEPRKRDKTSAP"
     gene            1811..2959
                     /locus_tag="PLF85_04815"
     CDS             1811..2959
                     /locus_tag="PLF85_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE19013.1"
                     /translation="MQNFDFQTTRRVVCAPGSIRQLPALARELGMTRALVVTDQGIFH
                     SGLYARCTDALRHGDVDFAVEHNVVADPPEAIVLEAVTKARALRIDGVIGFGGGSSMD
                     VAKVIATLVPSQQAIAEIYGIGNVRGSRLPLIQIPTTAGTGSEVTPIAIITTGETTKT
                     GIVSPQLLPDVALLDAELTVGLPADVTAATGIDAMVHAIEAYTSKIKKNPYSDLLAFE
                     ALKLLAGNLLRAVKQGDDLEARQAMLYGAMLAGQAFANAPVAAVHALAYPLGGHYHIS
                     HGLSNALMLPHVLHFNMPQAAQLYALLCGALPAANGLSPSGEKFAEALTQLISETGIP
                     RTLRELKVPRDNLPMLARDAMLQQRLLINNPREVTEADALRLYEEAWG"
     gene            2956..3411
                     /locus_tag="PLF85_04820"
     CDS             2956..3411
                     /locus_tag="PLF85_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19014.1"
                     /translation="MKPKKSIMLLLLAMGSAIPGVSAQASTTEKTPAGAIVEAPYEPD
                     YAKGLAERREWRRLRKIFLKKQLPFILRKHRDKLRCGTCMDVYMDIAFSVSLAGTISV
                     KQIDTAHACGGEFPDAMRADFLEYLRTYPYPAILLGKTVRLRLGTSLKC"
     gene            3440..4000
                     /locus_tag="PLF85_04825"
     CDS             3440..4000
                     /locus_tag="PLF85_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19015.1"
                     /translation="MRNLAVARRALLVLVAAAMPELRAERFAGESLEIGIERYQPVAP
                     TANANFTGLVINYYPSRYVYFGTNQMSLYVRGESDYRPDFHLGVIFPFTENFAAEAAL
                     GVDFYTALIIALAIADEDSNIDYKFTTNFYSPYFTFSTGLRYEFDAFTLKLMAQTQLG
                     GYLRDETSNFNASVWLGLGVTYRLAL"
     gene            complement(4005..4424)
                     /locus_tag="PLF85_04830"
     CDS             complement(4005..4424)
                     /locus_tag="PLF85_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNE19016.1"
                     /translation="MQQEHQGSCLCGAVKYNVKGPFIRFFFCHCSRCRRSTGTAHAAN
                     IFTRAGNVSFLSGEEKIKRFDLPEAAQFSRAFCTECGSSVPYVSRDGKVHVVPAGSLT
                     KFDERKVDANVHWDSRAAWYEGGLAAPRKTEEDLKKK"
     gene            complement(4447..5055)
                     /locus_tag="PLF85_04835"
     CDS             complement(4447..5055)
                     /locus_tag="PLF85_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNE19017.1"
                     /translation="MRDEGVTKYNCRFRRTQAPVHPCLNDLLGCRNRLFDQNLVGVYP
                     DGIGYGNVSLRPEGWRHFFITGTQTGHKPTLGAGDIALVTAYDIAANTVECEGLIAAS
                     SESMTHAAVYELSAEIGAVIHVHHRALWQRAAVHVPTTAAHIAYGTPAMAREMLRLCR
                     EAGLLQRGVLVMAGHEEGVITFGKDLASAEATLNQVLIDFTE"
     gene            complement(5057..7474)
                     /locus_tag="PLF85_04840"
     CDS             complement(5057..7474)
                     /locus_tag="PLF85_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE19018.1"
                     /translation="MPKHQPLSESALYRKCAPELIPFANTGEVAVSNEIFGQKRALDA
                     IAFGTSIDRAGYNIFVLGENGSGRHSAVQRILIDQARQNPSPQDVCYVHNFADANQPR
                     LLLLPAGRGVEFRNGMQQFVSELPKAITAAFESEEHRREIEAIIEEFRQREETALTEL
                     GRSSSDKGIALLRTQQGFLFSPIKGDSTLTAEEFEKLPDDEKDRLEKLMQEGTEKLHD
                     LMHKFPRWRREQQGRLKEVARKTMSYAVSHLVEELRDKFHDLPDVLSYLDAVLKDLIE
                     SGEALREQPRQEGDLSSLMIGEGTPIMRYYVNLVIDNASAQGAPVVYEDNPTLQNLMG
                     RIDYLAQFGMMITNLTLIKPGALHKANGGYLILDAVKLLTQPFSWDALKRALRSNHIR
                     IESLSQMYGFASSLPLEPEPVPLHLKVVLIGERMHYYLLRELDPEFAELFKVAADFDD
                     DIDRDNGNTGNYARFLANLAQSHKLRPFERSAIARLIEESARVAGDSGKLSVSARRML
                     DLMQEAEHFAAGAGKSLVDASDVRLALEAQIGRAARVREGIKDQIMRNLLLISLDEKR
                     IGQVNALVIIELADRMFGHPVRVTATARLGDGQVIDIERETDLGGSIHSKGVLILSAF
                     LASRYSRDIPLSLSASLVFEQSYGPVEGDSASLAELCALLSALSEVPIQQNLAITGSV
                     NQHGEVQPIGGVNEKIEGFFDICKARGLTGKQGVIIPESNVQHLMLREDIVAACGEKK
                     FAVYAVKTVDDAIELLTGRTAGRVESPGKKGSENLNYLVARRLEEFSLLRRQLKGSPG
                     KKNRKKR"
     gene            complement(7567..9375)
                     /locus_tag="PLF85_04845"
     CDS             complement(7567..9375)
                     /locus_tag="PLF85_04845"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19019.1"
                     /translation="MPTVSAAYELYINGMRLGGRGAIGAKGELQVVKEDVYLYQIPRR
                     LLRAGEINSIAFRIRSIDSVGGIYGDLDFLIGEYEQLYWHHLRAQVIFGLLAGAFFII
                     GLYYAVLYFSRREQKAPLYFAGLAVFLGLFILGMKGMTHIFTGSYAFHLTCIHLPILT
                     LPFWLLNFGFDFFEKPKTRMLKIVSFLSWFNVLLFSPVLVFPEKLLQLYLNSVFMLAF
                     LMVAYALVYFLYITYKAVRERKGGAFVVATGILFYFGCTTNDMLSYLNVIHSVRLSDA
                     GFLTLTFCMAVALAIQIARVYRDKEEAQRNALESQTLLADSYARFVPRQFLVNLGKES
                     ILDVKLGDQVQKQMAILFADIRSFTKLSESMTPAENFNFINSYLKRMTPIIQKHYGFI
                     DKFLGDGIMALFENSTEDSLNAAIDMQKYMVEYNQHRSKQNYPPINIGVGVHTGNLML
                     GTIGSEGRMDGTVISDAVNLASRIEGLTKLYGIKIATSDETLGTLKIPQAYNYRFIDR
                     VKVVGKDKPVGVIEVMDGDSAEQLEKKMATLPDFTEALAMYYRRDFDGARSLLSRVIG
                     RNPEDKAAKLYYERCAYFKEHGLPPDWEGVQALDSK"
     gene            complement(9433..9702)
                     /locus_tag="PLF85_04850"
     CDS             complement(9433..9702)
                     /locus_tag="PLF85_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19020.1"
                     /translation="MRKSLAFSLGYFSFLFLLSARAWSVGATENETLKIEKADTLYDL
                     AGDNWRFIAGDNPAYAEAAFDDSAWKKLNINNEWRLQGVDYDGVA"
     gene            complement(9813..10433)
                     /locus_tag="PLF85_04855"
     CDS             complement(9813..10433)
                     /locus_tag="PLF85_04855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE19021.1"
                     /translation="MPSSKPITVIDETSAVRCAETLAQAFSDNPGYVWAFPPEERREE
                     KFRWYMQRVLALGMINGLCLQIGDGDGVAIWYEPGRTAGFIDLMRAGLYKLPFRLGLG
                     IYLKIQKILNQNGHYMKKIMAGTPHYYLFHIGVTPAAQGHGFGSALIRYITDKADRER
                     KVCYLETDRPGNIPFYEKHGFVLVDSKELSPGVPLWYMRRPAAANR"
     gene            10528..10986
                     /locus_tag="PLF85_04860"
     CDS             10528..10986
                     /locus_tag="PLF85_04860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNE19022.1"
                     /translation="MAALARMAKPARSQRSDYSFFAPITTRWSDNDIYGHVNNVVYYS
                     YFDSVANLYLIEVAGLKIQSDNVVGFVVSSGCNYFSPVAYPEKLEGGLRANKVSGSSV
                     EYGVAIFRAGENEAVADGFFTHVFVDRTTGKPVRIPEAIRASLEKIQTPA"
     gene            complement(11035..>11864)
                     /locus_tag="PLF85_04865"
     CDS             complement(11035..>11864)
                     /locus_tag="PLF85_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19023.1"
                     /translation="CFMVGHFSPECSTGEQIVKDTHGAGYFFFHGYGPAPRWRMCLRI
                     FTLMLLVFIAQACLTIRNSKAIEEYRSPINEFDRPLPGGANYRILPGHMVVQNGRKLL
                     QFDSVLIGEKRFLNVESSAAGMRFYESAQRLDQGKKVFLIQQNACCMDDAAFRRILFL
                     KPGEKTTAAEILKTYYGLESGATTYPDALLVMDFTNIHTFSAMHGYWQSEAGLSYLVQ
                     APSTDYNEVMREIEWRQRSRISLAAMYGWYAVTVPVDIVTSPFQLIGMYLLGKGMVH"
BASE COUNT         2721 a         3341 c         3195 g         2607 t
ORIGIN      
        1 cggttattcg ctggcgctga ttgcatggcg ctggtttcat tcgcggcgca gggccgaccg
       61 cagtatcgtg atcatcgtcg cagcgctgat gctgggtgcc gcgttcatgc tcacgaacgt
      121 tcctggtaca cagggtatat cttttccgcc actcggggcg ctgcagttca tacccgccgg
      181 aatgattgct tttgccatct tgcgctatcg cgcgatcgtg atacgcggtg aggcaacgcg
      241 tattgcaaac cggttccagc tgatgagcct cgtcggcgtc ggttttctcg tctttgttta
      301 tgcgcgcacc ctgcctgaag ggcttgagac tatcaaaatc tggaaccacc tttcggttat
      361 cgctgcttcg ctgctgctcg gctttagtat gctgctattc gtactggtac gtccggtagc
      421 agcggccatg gacgacagtt tccacctgct ggacgaacaa aaaaagcaac tggaagaaag
      481 cgaaaagaac ctgcgggaaa tcgccgatgt ttcgcgctct gtctacgcct cggtcaacct
      541 gaacggcatc atcgacctga tgttcgccta cgtgcagcag cgctttggca tcgaaggact
      601 ctggctcact ttttgcgata ctgcgcagaa ccgcatctac cacgtgcgcc ataacgcaga
      661 cacccaacta ccacgcgaga cactcggctt catggagcaa ttcagcgttg atctcacgcc
      721 cgatgcgggg ttgctttacc atacctggca gcgcaaacgc atcacgtaca tgcggcgcct
      781 ggcctgtccc gacaagatgg cggagcttga ccgtatactg gcagaaaaac tcgacctgaa
      841 gggatatgtt cacgtgcctc tcgtgttgca gaatgaagtg attgccgtgc ttatgggcat
      901 gaatttcggc aagaagctgc atctgtcacc gaaagacctg cgctcgctcg aactcttcgc
      961 cgggcagatc gcaggagcat tgtcaacagc ccgcctgctg aaagagatca accaggaaaa
     1021 aaagaaatcc gacgagctgc tgctcaacat tctgccgcgc ggtattgctg accagctaaa
     1081 agagcgcggt gaagtaacgc cgcaattcta cgattctgtc acgatactct ttaccgactt
     1141 tgtgggcttt acgcgttcgg cgcttactat ggtgccgacc gaactgatcg aacagctgaa
     1201 cagaattttc taccagtttg atgccattac gcggcggtat aaccttgaaa agatcaagac
     1261 catcggcgat tcgtacatgg cggccggcgg tttgcctgag aaaaatgcga cgcactacct
     1321 cgacgtctgc ctcgcggcgc tcgaaatcgt caagatgacc gaacaggtca tcgcgatgtc
     1381 aggcggtacc agtacggaaa gctggcaact acgcgtcggt attcataccg gccctgtgat
     1441 ggcaggcgtc gtaggccagt acaagtttgt ctacgatatc ttcggtgacg cggtaaacgt
     1501 cgcgagccgc atggaaacga ccagcgaggc tgcacgcatc aacatatctg cagatactta
     1561 ccacaaggtg aaatttttct ttgactgtga ataccgcggc cgtatacata tcaggaaccg
     1621 cggcgaagtg gaaatgtact ttctcaacgg cctgaagccc cgtttcctga caaaacacgg
     1681 cgctcccaat gcagaatttc gcgaaatcta cgaaaaaatc cgtaacgggg cgcgcattga
     1741 gccgcggaaa cgggacaaga cctcagcccc ttagcgcttg acgtcgcgcg actggactga
     1801 acctgcaggc gtgcagaact tcgacttcca gaccacacgc cgcgtcgtct gtgcacctgg
     1861 ctccatcagg caactgccgg cgctcgcccg cgaactgggt atgacgcgcg cgctcgtagt
     1921 cactgaccag ggcattttcc attcggggct gtatgcccgg tgcacagacg ccctgcggca
     1981 tggcgatgtc gactttgccg tcgagcacaa tgtcgtcgcc gacccgcccg aagccattgt
     2041 gctcgaagct gtaacgaaag cgcgtgcgct cagaattgac ggagtgattg gcttcggcgg
     2101 cggcagctcg atggatgtag ccaaagttat tgccacgctt gtaccttcgc aacaagccat
     2161 cgccgagatt tacggcatcg gcaacgtcag ggggtcgcgg ctgccgctca ttcagattcc
     2221 gaccacggcc ggcacggggt cagaggtaac tcccattgcg attattacga ccggtgaaac
     2281 gaccaagacc ggtattgtct cgccacaact tctgccagac gtggctctgc tcgatgcaga
     2341 gctgacagtc gggttaccgg ccgatgtgac cgcggcaacg ggcatcgacg cgatggtgca
     2401 cgcgatcgaa gcttacacca gcaagataaa gaaaaacccc tactctgacc tgctggcctt
     2461 tgaagcgctg aaactcctgg caggtaacct tctgcgcgcg gtgaagcaag gtgacgatct
     2521 ggaagcgcgg caggcaatgc tttacggtgc aatgctcgcc ggccaggcat tcgcgaatgc
     2581 ccctgttgca gcggtacatg cactggcgta cccgctgggt ggtcattacc acatctcgca
     2641 cggtttatcg aatgcgctga tgctccccca tgttctgcac ttcaatatgc cgcaggcggc
     2701 gcagctttac gcgttattgt gcggagctct gccggcggca aacggtctca gtccgtcagg
     2761 cgaaaaattt gccgaggcac tgacgcaact gatttctgaa acaggaattc cccgtacgct
     2821 gcgtgaactc aaggtaccgc gcgacaacct gcccatgcta gcgcgcgacg cgatgctgca
     2881 gcaacggctg ctcatcaaca acccacgcga agtgaccgaa gcagatgcgc tcaggctcta
     2941 tgaagaggcc tggggatgaa accgaagaaa agtataatgc tactgctgct ggcaatgggt
     3001 tcagcgatac ccggtgtttc ggcacaggct tcgacgactg aaaaaacgcc tgcgggtgcg
     3061 atcgtcgaag caccctatga gcccgattac gcaaagggac tcgccgaacg ccgtgaatgg
     3121 cgacgcctgc ggaaaatctt tctcaaaaaa cagctgccat tcattctgcg caaacaccgc
     3181 gacaagttgc gctgtggcac ctgtatggat gtctatatgg acatcgcgtt ttcggtcagc
     3241 ctcgctggca caatctctgt gaagcagatt gatacggcgc atgcctgcgg gggtgaattt
     3301 cccgatgcga tgcgcgcaga ttttctcgaa tacctgcgca cataccctta cccggccata
     3361 ctgctcggca agaccgtgcg gttaagactc gggacgtcgc ttaaatgctg agcgcaatgt
     3421 tcaaaaattg ccgggagcta tgagaaatct ggctgttgcc cgcagggcgc ttctggtgct
     3481 ggttgccgcg gcaatgccag aacttcgggc cgagcgcttt gcgggcgaat cactggaaat
     3541 tggcatcgaa cgctaccagc cggttgcccc aaccgcaaac gcgaacttca caggtctcgt
     3601 catcaactac tacccttcgc gctacgtcta ttttggtacc aaccagatgt cactctatgt
     3661 ccggggagaa tcggactacc ggccagactt tcacctgggt gtgatatttc ccttcacgga
     3721 gaattttgct gcagaagcag ccctgggcgt cgacttctac accgcactta ttatcgcgct
     3781 cgccatcgcc gacgaagaca gcaacatcga ttacaagttc acgacgaatt tttactcgcc
     3841 ctactttacc ttttcaacgg gactacgcta tgaattcgat gcgtttacgc tgaagctgat
     3901 ggctcagaca cagctgggcg gctacctgcg cgatgagacg agcaacttca atgcttcggt
     3961 ctggctgggg ctgggcgtga cctaccgtct cgcgctttga cgggctattt tttcttcagg
     4021 tcttcttcgg ttttgcgcgg tgcggccagg ccaccttcgt accaggcggc acggctgtcc
     4081 cagtggacgt tcgcatctac cttgcgttcg tcgaatttcg tgagcgaacc tgcgggcacc
     4141 acgtgtactt tgccgtcacg gctcacgtag ggcactgatg aaccgcattc ggtacagaac
     4201 gcgcgtgaaa actgcgcagc ctcgggcaga tcaaagcgtt taattttttc ttcgcccgaa
     4261 agaaagctca cgttacccgc acgcgtgaaa atgtttgccg catgcgctgt accggtcgaa
     4321 cgccggcagc gcgaacaatg acaaaagaag aacctgatga atggtccctt gacgttgtac
     4381 ttcaccgctc cgcagagaca gctaccctgg tgttcctgtt gcataccgta tacaccctgt
     4441 gggagcttat tctgtaaagt cgattaatac ctggttgagg gttgcctccg ccgatgcgag
     4501 gtctttgccg aacgtgatga ctccctcttc gtgacctgcc atgacaagca cgccgcgctg
     4561 cagaaggcct gcttcgcggc agaggcgcag catctcgcgc gccatcgcgg gagtaccata
     4621 ggcaatgtgg gctgctgttg tcggtacatg cacggcagcg cgttgccaca acgcgcggtg
     4681 gtggacgtgt atcacagcgc cgatttctgc cgagagctcg tagactgccg catgcgtcat
     4741 cgactccgat gaagctgcaa ttaatccctc acattcgacc gtgttcgcgg caatgtcgta
     4801 ggctgtgacc agggcaatat cgccggcacc gagtgtgggc ttgtggccgg tctgcgttcc
     4861 cgtgataaaa aaatgacgcc aaccctctgg ccgcagcgag acattgccat agcctatacc
     4921 gtcgggataa acaccgacca ggttttggtc aaaaagtctg ttacggcaac caagcaggtc
     4981 attcaggcaa gggtgaacag gggcctgcgt acgacgaaac cggcagttgt acttggtgac
     5041 accctcgtcg cgcatctcag cgcttcttgc ggttcttctt gccggggctg cccttcagct
     5101 gccggcgcaa gagcgaaaat tcctcgagcc ggcgggcaac caggtagttc aggttctcgc
     5161 tccccttttt gccggggctc tctaccctgc ctgcagtgcg gccggttaag agctcaatcg
     5221 cgtcgtcgac agtcttcacg gcatagaccg cgaacttctt ttctccacag gctgcgacga
     5281 tgtcttctcg cagcatcagg tgctgcacgt tcgattcggg aataataaca ccctgcttac
     5341 cagtcagccc gcgcgccttg cagatatcga aaaaaccttc gatcttctcg ttgaccccgc
     5401 cgatcggctg cacttcgccg tgctggttca ctgaacccgt gatagcaagg ttttgctgta
     5461 tcggcacctc tgagagggct gaaagcaggg cacagagctc ggcgagcgag gcgctgtcac
     5521 cctcaaccgg cccatacgac tgctcaaaaa cgaggcttgc gctcagcgaa agcggaatgt
     5581 cgcgcgaata gcgcgaggcg agaaatgcgg acagaatcag aactcccttc gagtgaatgg
     5641 aaccgcccag atcggtttcc cgctcaatgt caatgacctg cccgtcgccg agccgggcgg
     5701 ttgccgtcac gcgcaccggg tgaccaaaca tgcggtcggc cagttcgatg atcacgagtg
     5761 cattcacctg cccgatacgt ttttcatcga gcgagatcag caagaggttg cgcatgatct
     5821 ggtctttgat accctcgcgc acacgcgccg cgcggccaat ctgcgcttcg agcgccaggc
     5881 gcacgtccga ggcatcgacc aggcttttgc cggcgcccgc ggcaaagtgc tcggcctctt
     5941 gcatcaggtc gagcatacgc cgcgccgaaa cgctgagttt tcccgagtcg cccgcaaccc
     6001 gcgcgctctc ttcgatgagg cgcgcaatcg ctgaacgctc gaacggtcgc aacttatggc
     6061 tctgcgccag gttcgcgaga aagcgcgcgt agtttccggt attgccgttg tcgcggtcaa
     6121 tatcgtcgtc aaaatctgcc gcaactttga acagctcggc aaattcaggg tcaagttcgc
     6181 gcagcaggta atagtgcatg cgttcgccga tcagcacaac tttcaggtgc agcggtacag
     6241 gctcaggctc gagcggcagc gaactggcaa aaccgtacat ctggctgagc gactcgatac
     6301 gtatatgatt tgaacgcaat gcgcgcttga gggcatccca cgagaagggc tgggtcagaa
     6361 gcttcaccgc gtcgagaatc aggtaaccac cgttcgcttt gtgcaaagcg ccgggcttga
     6421 tcagcgtcag gttcgtgatc atcataccga actgcgcgag atagtcgata cgccccatca
     6481 ggttctgcaa ggtcggattg tcttcgtaga cgacgggagc tccctgcgcc gaagcattat
     6541 cgatcacgag gttgacatag taccgcatga tcggcgtacc ttcgccgatc atcagcgaac
     6601 tcaggtcacc ctcctgccgc ggctgttcgc gcaacgcctc tcctgattca ataagatctt
     6661 tgagcactgc atcgagataa ctcaacacat caggcaggtc atggaatttg tcgcgcaact
     6721 cttcaacaag gtgtgaaacc gcataactca tcgtcttgcg ggcaacttct ttgagacgtc
     6781 cttgctgctc gcggcgccag cggggaaatt tatgcatcag gtcgtgcagc ttctccgtgc
     6841 cttcctgcat cagtttttcg agacgatcct tttcatcatc gggcagcttc tcgaattctt
     6901 cggcggtgag cgtcgaatcg cccttgatgg gagaaaaaag aaaaccctgc tgtgtccgca
     6961 gcagcgctat acccttgtct gacgaggagc gcccgagctc ggtcagcgcc gtttcttcgc
     7021 gctggcgaaa ctcttcgata attgcctcga tctcgcgccg gtgctcttcg ctctcaaacg
     7081 ccgcggtgat tgccttgggc aattcagata cgaactgctg catgccgttt cggaactcaa
     7141 ctccccggcc ggcgggaagc agcaacaagc gtggctggtt tgcatcagca aagttgtgca
     7201 cgtagcagac atcctgcggc gaagggttct ggcgcgcctg gtcgatcagt atacgctgca
     7261 cggccgaatg cctgccgctg ccattttcgc cgagcacaaa aatgttgtaa ccggcacgat
     7321 ctatgctggt accgaaggcg attgcgtcga gcgcgcgctt ctggccaaaa atttcgttac
     7381 tgactgccac ctcgcccgta ttggcgaacg gtatcagctc tggggcacac ttgcggtaaa
     7441 gcgcgctttc ggagaggggt tgatgttttg gcatagtcgg gacaaccaca gatgccagtt
     7501 gagcatctac gggtaaatcg caaatcagcg tcggctttac gagagttccg gaacaggctc
     7561 aagaaatcat ttgctgtcca gcgcttggac accttcccaa tcgggtggca ggccatgctc
     7621 tttaaaatag gcacagcgct cgtaatagag ttttgcggcc ttatcttcag ggttgcggcc
     7681 gattacccgg ctcagcagag aacgcgcccc gtcaaaatcg cggcggtaat acatagccag
     7741 ggcctcagta aaatcgggca acgtcgccat tttcttctcg agttgttcgg cagaatcgcc
     7801 gtccataact tcaatcacgc cgacgggctt atctttaccc acaaccttca cccggtcgat
     7861 aaaccggtag ttatatgcct ggggaatttt cagcgtaccc agggtttcgt cgctggttgc
     7921 aattttgata ccgtagagtt tggtcaggcc ttcgattcgc gacgccaggt tcaccgcatc
     7981 ggaaatcacc gtgccatcca ttcggccctc tgaaccgatc gtaccgagca tcaggttgcc
     8041 ggtatgcacg ccgacaccga tattgatcgg cggatagttc tgcttcgacc ggtgctggtt
     8101 gtattcgacc atgtactttt gcatgtcgat tgccgcgttc agcgaatctt cggtgctgtt
     8161 ctcgaagagc gccataatgc cgtcgccgag aaatttatcg ataaacccat aatgcttctg
     8221 gataatcggg gtcatacgct tgaggtatga attaatgaag ttaaaatttt cggccggagt
     8281 catcgactcc gaaagtttgg tgaaagacct gatatcggca aagaggattg ccatctgctt
     8341 ctggacctgg tcgcccagct ttacgtcgag aatagattct ttgccgaggt ttaccagaaa
     8401 ctgccgcggc acaaaccggg catacgaatc ggcgagcaga gtctgcgatt caagtgcgtt
     8461 gcgctgcgct tcttctttgt cgcggtagac cctggcaatc tgtatcgcca gcgccaccgc
     8521 catacagaat gtcagcgtca ggaaacctgc atcgctcagc ctgacagagt ggataacatt
     8581 gagatacgag agcatgtcgt ttgtggtgca gccgaagtag aaaagaattc ctgtggcgac
     8641 aacaaacgcc cctcccttgc gctcgcggac agccttatag gtaatataaa gaaagtagac
     8701 taacgcataa gcaaccataa gaaacgccag catgaacacg ctgttcaggt agagctgcag
     8761 aagcttttca ggaaatacca gaaccggtga aaacagcaga acgttgaacc aggaaagaaa
     8821 gctcacaatc ttcagcatgc gggttttggg cttctcgaaa aaatcaaagc caaaattcaa
     8881 caaccagaaa ggcagtgtca gtatcggcag gtgtatacag gtcaggtgaa aggcgtacga
     8941 tccggtaaaa atgtgcgtca tgcccttcat gccgagaatg aatagaccga gaaagacagc
     9001 aagaccggca aaatagagtg gcgccttttg ttcgcggcga gaaaaataca gcacggcgta
     9061 atacaagccg atgatgaaaa aagctcccgc gagcaggcca aatatcacct gcgcacgcag
     9121 gtggtgccag tacaactgtt cgtattcgcc gatcagaaaa tcgaggtcgc cgtagatgcc
     9181 gccgacgctg tctatggaac ggatgcgaaa ggcaatggaa ttgatttcgc ctgcgcgcaa
     9241 gagacgccgc ggaatctggt acaggtacac gtcttctttg acaacctgca actcgccttt
     9301 ggcgccaatg gcaccgcggc caccgagacg cataccgttg atatagagct cgtaggctgc
     9361 agagaccgtc ggcaccaaca ggccaatttt tttatcggca aactgctcac cgataaataa
     9421 tttttgtctg aatcaggcga cgccgtcgta gtcgaccccc tgcaggcgcc attcgttgtt
     9481 gatattcagt tttttccagg cagagtcatc gaaggcagcc tcggcatatg cggggttatc
     9541 gcccgcgata aagcgccagt tgtcgccggc gagatcgtaa agggtatcgg ccttttcaat
     9601 cttcagcgtt tcgttctctg ttgcgccgac agaccaggcg cgtgccgaga gcaaaaaaag
     9661 aaacgaaaaa tatccgagag aaaaagcgag gcttttacgc attggcaaca ctctcatagc
     9721 ggcagtatcc ggcaaacaca aatgcccgcc ggggtttgaa gcgcattttt ttcagcaaca
     9781 aaaagccaaa gaattactgc aacagggcag aattaccgat tggcggcagc agggcggcgc
     9841 atgtaccaga gcggtacgcc gggcgaaagc tctttactgt ccaccagaac gaaaccgtgt
     9901 ttttcataga aaggaatatt gccggggcga tcggtttcga gataacaaac cttgcgctcc
     9961 cgatctgcct tgtcagtgat atacctgatg agcgcgctgc caaaaccatg cccctgcgcc
    10021 gcgggcgtaa cgccgatgtg aaaaagatag tagtgagggg taccggccat gatttttttc
    10081 atataatgac cgttttggtt cagaattttc tgaatcttca gatagatacc caggcccagc
    10141 ctgaaaggaa gtttgtagag gcccgcgcgc atgagatcga tgaaaccggc agtgcgaccc
    10201 ggctcgtacc agatggcaac accgtcaccg tcgccgatct gcaggcaaag gccgttgatc
    10261 attcccagcg ccagcactcg ctgcatatac caacgaaatt tttcttcgcg ccgctcttct
    10321 ggcggaaaag cccacacata cccaggatta tcagaaaagg cctgcgccag tgtttcggcg
    10381 cagcgaacgg ctgaggtttc gtcgattacg gtgataggtt tactgctcgg catcggacgg
    10441 gaaagctgta cagacaggta gtcgtgtcaa taccaatttg cagagcctca gtccagagca
    10501 tggtaaggta ttgacaatgc aaaaaaaatt gcggcactcg cccgcatggc aaaacccgcc
    10561 cgttcgcagc gcagcgatta ttcttttttt gctccgatca ccacgcgctg gtctgataac
    10621 gatatctatg gccatgtcaa caatgtcgtc tattactcct attttgacag cgtcgctaac
    10681 ctttacctga tcgaggttgc gggcctcaaa atccagagcg acaatgtcgt cggttttgtg
    10741 gtcagctcgg ggtgcaatta tttttccccc gtggcgtatc ctgaaaaact ggaaggcgga
    10801 ttaagagcga acaaggtcag cggatcatcg gtcgaatatg gcgtcgcaat tttccgcgcg
    10861 ggtgaaaacg aggctgtggc tgacggcttc tttactcacg tctttgtcga ccgcactacg
    10921 ggcaaacccg tgcgcatacc cgaagctatt cgcgcgtcgc tcgaaaagat acaaacgcct
    10981 gcttaactga tgctgttctc accgctgcag aacataatct gcgcccgcgc aacttcagtg
    11041 caccatccct ttgccgagca aatacatacc gatcagctga aaaggtgaag tcacaatatc
    11101 gacgggtacc gttaccgcgt accagccata catcgcagca aggctgatgc gactgcgctg
    11161 ccgccattct atctcacgca tcacttcgtt atagtcagtc gacggggcct gcacaaggta
    11221 acttaacccg gcttcgctct gccagtaacc gtgcattgca ctgaacgtat ggatgttggt
    11281 gaaatccata accagcagcg catccgggta cgtcgtcgcg ccactttcga gcccgtaata
    11341 ggttttgagt atctcagccg cggtggtctt ctcgcctggt ttcaaaaaga ggattctccg
    11401 aaacgccgcg tcgtccatac agcacgcgtt ctgctgtatg agaaaaacct ttttaccctg
    11461 atccagccgc tgcgctgatt cgtaaaaccg catgccggcc gctgacgatt caacattcag
    11521 aaagcgtttt tcaccgatca gcacagaatc aaactgcagc aacttccgac cgttctgtac
    11581 caccatgtgt ccgggcagaa tgcgatagtt agcgccgcca ggcagcggcc ggtcaaattc
    11641 attgatcggg gatcggtact cttcgatcgc cttgctgttt cgaatcgtca ggcatgcctg
    11701 ggcaataaac accagcagca tgagggtaaa aattcgcagg cacatacgcc aacgtggcgc
    11761 aggcccgtaa ccgtgaaaga agaaatagcc agcgccgtga gtatctttca cgatctgctc
    11821 gccggtcgaa cactccggtg aaaaatggcc caccatgaaa cagt
//
LOCUS       DAOQJF010000060        11722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2756_length_11722_cov_4.733865, whole genome shotgun sequence.
ACCESSION   DAOQJF010000060 DAOQJF010000000
VERSION     DAOQJF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_11722_cov_4.733865"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLF85_04870"
     CDS             <1..350
                     /locus_tag="PLF85_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19024.1"
                     /translation="NGLSQSMENRVQWHVPAYAHAFYRLDKLLALGAGLTHLTETRMY
                     LDSQPVPESSYNHLFIDAALQVRPKLSENLNAVITAVVGINVIPGRQHTYSVGDLLHL
                     RFQLMAGMVYAAF"
     gene            353..1153
                     /locus_tag="PLF85_04875"
     CDS             353..1153
                     /locus_tag="PLF85_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNE19025.1"
                     /translation="MRTVILRLIALTVLTVLLIFFWDSALLKPFKLFVVFLHETSHAI
                     ATIATGGQLHSISIEWDESGATYARGGEGVFVIIALAGYIGSIIWGYLMLRASLTGRW
                     IRSVSVIVGLTVIFFGFFPDGETPRQSDKMLKYLISGSWGLVLTVSAFIYPRFNHFIL
                     FFLGGLTSLYSLYDLNDFIRGDVMRTDAGILAHYLLGQSTLVKPLAYVIAGFISSVSV
                     FIFLRLLRATFHDVPEPEEVDLAEWQAQNPDVTITPEMLEWLKQQSKR"
     gene            1200..2351
                     /locus_tag="PLF85_04880"
     CDS             1200..2351
                     /locus_tag="PLF85_04880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE19026.1"
                     /translation="MRVSSGIQGELDLINRIFCDDEQSEEQILQTVEELYEAGEKNIY
                     ARLLHVMCHLRLAPAEARKSWQAILRHRDLIESKLGRKTGFRVAMLDYFVNESRDIKN
                     PKVIEIHVYAETARQAAIDELTGVFNRRFFDGAIERECKQAQRHKREFSLLILDIDNF
                     KRINDTHGHTTGDAVISALGKLLKKSIRGEDTACRIGGEEFAVILPETGPEGAEVVSE
                     KIRSEFAKLKIASTELSLSGGLATFPLDAQSTEDLIDRADQMLYFAKYSGKNRVICYS
                     RNKRRHIRYPLEWELSISRPLGEQGKTLSQDVSIEGISFELDDAAHPGDLMSLHLNVD
                     AEPIELPVQIVWVDNNSQNRRFRAGARFIRLTEKNLHSLKQKLPRHGVV"
     gene            2517..3437
                     /locus_tag="PLF85_04885"
     CDS             2517..3437
                     /locus_tag="PLF85_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19027.1"
                     /translation="MRSFKHIKTLPFKALLFVIILGSPAVQAAAPDQGFVEKILRENR
                     YFIEFANVSVSNFGSPENEKSLKQANQHNFNANLYYLESNYVDAFKEIRLSQEILLDL
                     FYDVLQKRYIEDSRTLLDLNAPIIIQSKDRKSAHFLQLGYRDVEVARQFRDMGYNYNR
                     FLFSNKIRYYIDGIKRARRAKRFAFLSLIESKTPIEDKVDYQTQTWDDARNKDDRENI
                     RDYERVKNNLVNQMNRKLYSDAMNFFLHHDDNYGLISGQKKSLLKDSFKELQTKRPEK
                     RARDPEFPAKDQPEVKKAEPAKTEPAPAKK"
     gene            3491..4060
                     /locus_tag="PLF85_04890"
     CDS             3491..4060
                     /locus_tag="PLF85_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19028.1"
                     /translation="MPIAATFAGPLDIQKLRLKGAQNSKELRHFLETGLVAQRQTPIE
                     MQIENFERSLTAYKEAKHTERNAALRQVQNVYEKFQVEFQAVADILRALVDKHQAAYN
                     ERSSEAFANTALREKVLSTVEVGKQEEVRALAAYNSRNFSYSAHLYLRSLRHYTNAFE
                     LRGWQPLVIMPAKPGKKPKAEKTAPAAPR"
     gene            complement(4064..4954)
                     /gene="gspN"
                     /locus_tag="PLF85_04895"
     CDS             complement(4064..4954)
                     /gene="gspN"
                     /locus_tag="PLF85_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HNE19029.1"
                     /translation="MTENENTEDLQTPAPPPEPERTWQSTLKRFAPVLAGLAAFLVGL
                     VWFAPLEAYTILALRQLAASGYHVEVGDLSISAFGRYKVESLKIPLAGDGDKQGNLKI
                     AEAKGRVALLSALLGDKYDLTTEAVILSFAKGDFALKIDSLEIKSLLEQTKSGGTGKL
                     LNGTVSIEAESAQVTYKENRFLKEEIVLPFLKIVLKARAQQNQVAIETGEAMGRLVNA
                     QIRGSLNLGPQTELNLNLVIKPTEEFYQKYQDKDPKTLLKFANVLQDDGRIEFNIKGT
                     VAQPVVEPVTAVAVKPPTAP"
     gene            complement(4951..5472)
                     /gene="gspM"
                     /locus_tag="PLF85_04900"
     CDS             complement(4951..5472)
                     /gene="gspM"
                     /locus_tag="PLF85_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNE19030.1"
                     /translation="MKKFWESLNPRERTYLMILGVFALFLGVFFAGRSFAAYMTDLRE
                     RTDSAERDTVQIENLGRQFQVLQAMKTGDSQQLDNMLSSIETMLKNIGLREKVGSLTP
                     TDTIVQEKYLKREVKVSIKESPANLVLDFIRQVEQSVQTLYKVESFSYRPVLKKPGIY
                     DFTVQISGFQRKQ"
     gene            complement(5476..7125)
                     /locus_tag="PLF85_04905"
     CDS             complement(5476..7125)
                     /locus_tag="PLF85_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19031.1"
                     /translation="MARTPVVIEINNYEVKGLAFRSGIAGRSFFQAETMVLNPKESIP
                     AQVAAFVAERYPGAIGVHFNVPYESLITRELSFPFADRRKVREVLPFELEPLIPHDLQ
                     DVVYDSYLTTNLANQSTRAIVAGSRKDTLIRYLDEFRQRQVTVLGIYTPHDALYRLHP
                     FTGLESCIMLHVSRMVSIIVIVLAGEWRFARVMPVGYDWLIARLKGKWKSDVADIHRI
                     LLALPASLDPSPDLEAFKKTHKISKDRFKSLQDSIHDFTQLISRELEVSLRNLQIDQE
                     GDAAQVKPEQENAESKPLNPSELPVSLSSDLNNAMVIENALARGLNHEVVPFPYERTP
                     LALLDREHLVTMGGAYSQTVSGKMNLLKDELRKWVVSSQAGASRIVLALAAAAVVIFS
                     LSFFIDLRRRSQQLKEWEDRQAKVFQQFFPGTQLPDNVSALSYAAELVRKEKQKTEII
                     EVFLKAPRLSAVLVQLHQNISTEAGLEITSFYYSDRGVEINGLVPDFAKLEDIKRSLG
                     KASLLTAVESKNENSTPGPGGQNRIKFTLFCKVKKTEGQTQ"
     gene            complement(7128..8099)
                     /locus_tag="PLF85_04910"
     CDS             complement(7128..8099)
                     /locus_tag="PLF85_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNE19032.1"
                     /translation="MALVYVRFALNEYRAVTTTASRERTLQLAQSGLETGNLLLTRIP
                     LDSLYEFGIFTIAPTIPLGGGTVTLSLSEETGKLNVNRLVRMFEDQTDERHFEYFQRL
                     SVALGIPVDLWHAVVDYIDENNTPMPRGAERDDYERLMPPRRIKNGRMQTIEELLFVP
                     GFDARLLYEDLRSLDKKKNQATAGLSDLEKATLTPEDYILANNITTHLVYNADTSTDQ
                     ININSAPYHVILSLSEFMTPAAARKIIAERIKRNGRFKSRDEIASLPEMQIKTRGNLS
                     LFQEILDRVTTDNQLYKIVADASMDSQTAQVMGILDPNAKRLVFYSE"
     gene            complement(8206..9042)
                     /locus_tag="PLF85_04915"
     CDS             complement(8206..9042)
                     /locus_tag="PLF85_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNE19033.1"
                     /translation="MNRVFLALLLCACATRAAERPYLSSGAHWKLVTHLTCKVEAEDE
                     KKIVPRAYRYGTSAKNRPLIAHIVEPAPGGAEDADNIYIIGAQHGDERNTKRVLEYLL
                     REIRLMSRDYRNDHRIIVIPNYNPDGYARNQRVSATGVDLNRDFPSSDGDEENPKAVE
                     TQAFMRLMRKYPPYVIYNLHQPFRVVLFYPGDEEIAKPFAQLSDYPLGQGVGYPTPGS
                     LGTYGREKKIPVVTVELSRSMKPAMAPFIFEEVRLALFNAAFGCIPKQAHRSRIEKYL
                     AE"
     gene            complement(9039..10196)
                     /locus_tag="PLF85_04920"
     CDS             complement(9039..10196)
                     /locus_tag="PLF85_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE19034.1"
                     /translation="MFDLFNPTDDHKSLRELTHDLAKGVLEPQVKEYDDKEILNEALL
                     RKMGSEYSLFGITVPAEAGGHGLDATASVIVHEELSYSDAAFTLSYLAHEVLFVNNLY
                     HSAQPGLRERYLSQVLDGSALAGMAMSEPAAGTDVLGLTTVAEKKGDRYILNGVKQWI
                     TNGNCGSYFIVYARTEKGNRSAISSFVVEKNFKGFSVGKKEIKMGMKQSPTCQLVFED
                     VEVPAENLIGKESGALKHMMRNLEIERLTLAAMSLGIARRAIDEMVRYASVDRMAFGK
                     HLADFGQIQQLIAESYARYRASRALVYETALRVNPESRESLSAAAAKLSATQMAEFVA
                     RAAIQVFGGYGYTREYPVEKLLRDAILLSIGGGTNEAMQKNIMKDLRDSLK"
     gene            complement(10251..11345)
                     /locus_tag="PLF85_04925"
     CDS             complement(10251..11345)
                     /locus_tag="PLF85_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE19035.1"
                     /translation="MKQQIRSNNHLYFTLVMLLPLAGCLANPQAERFDLNQSVGKITV
                     NDVDIQVKFAPVALVGNVIEARAQRPTLEVSFVSRNSSPVTLNFKLKNMRHDTLPTAS
                     FGTITAGEKAGIVNWALTIAAGETRNVTVANFTGGGAYRFGVVGDLHFRDSTASRIAE
                     SARSRGLDFFLLVGDFVNQPNSDEYSWALALSDKFAGPVYTAYGNHEGFREGYMNFYR
                     GLFGTTNYAFTHKGDFFLVLDSADQSISREVYRYAESELNQSGSALKMMFMHVPPFDE
                     FGMRNNSFNSRFQAARFLNMALDKGVDIVFAGHIHTYQEFWVDAIRNVIVGTGGGRPE
                     QFDGIGIRYVILHSDGAGNYQLETVDLGDD"
     gene            complement(11342..>11722)
                     /locus_tag="PLF85_04930"
     CDS             complement(11342..>11722)
                     /locus_tag="PLF85_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19036.1"
                     /translation="LNALAEFTLYPENLTSYYAKSLRGIGIFTTVGIGRQYIHLRDTP
                     DFRKDAHFANLVLMHGVRFRATRFTEFSSAFNFRKDNILGGASILGGTFVHQVEIFLG
                     NFFTRITLEHGRGYRLFALAGVNW"
BASE COUNT         2501 a         3165 c         3361 g         2695 t
ORIGIN      
        1 tcaatgggct atcgcagagc atggaaaacc gcgtgcagtg gcatgtgccc gcctatgcac
       61 atgctttcta ccggctcgat aaactgctcg cgctgggggc aggcctgacg catctgacag
      121 aaacccgcat gtatctggat tctcaaccag tgcccgagtc gagctacaat cacctgttca
      181 tcgatgcggc gctgcaggtg cgcccgaagc tgagtgaaaa tctgaacgcc gtgatcacgg
      241 ctgttgtggg tatcaatgtg attcctggcc ggcagcatac gtattcagtg ggtgacctgc
      301 tgcacctgcg ttttcagctc atggccggta tggtctacgc ggcattctga tcatgcggac
      361 tgttatcctc aggctcatcg cgctgaccgt gcttacggtt ttgctgatct tcttctggga
      421 ctctgcgctc ctgaaacctt tcaagctgtt cgtggttttt ttacacgaga cctcgcacgc
      481 gattgcaacc atcgcaacgg gcggtcaatt gcactcgatc tcgatcgagt gggatgaatc
      541 cggtgccacg tacgcacggg gtggcgaggg agtttttgtc atcatcgcgc tcgccgggta
      601 tataggcagc attatctggg gctacctgat gctgcgcgct tcgctcaccg gccgctggat
      661 acgctctgtt tccgttatcg tcggtttgac ggtgatcttt ttcggttttt ttcccgacgg
      721 cgaaacgccg cgccaatcgg acaagatgct gaaatacctg attagcggct catgggggct
      781 cgtgctcacc gtcagcgcct tcatttaccc gcgtttcaat cacttcatcc tgttcttcct
      841 tggtggcctg acctcgctct attcgctcta tgatctcaat gattttatcc gcggcgacgt
      901 catgcgcaca gatgccggca tactggcgca ctacctcttg gggcaatcga cgctggtgaa
      961 gcccttagct tatgtcatcg cgggctttat cagctctgtg tcggtattca ttttcctgcg
     1021 cctgctgcgc gcgacattcc atgacgtacc cgaaccggaa gaagtcgatc tcgctgaatg
     1081 gcaggctcag aatcccgacg ttacgatcac tcccgaaatg cttgaatggc tcaagcagca
     1141 gtcaaagcgg taaaacgggt ttcgccttta caccccgtat cggtcgcatg ctgaaaggca
     1201 tgcgcgtctc gtcaggcatc cagggagagc tagacctcat caaccgcatt ttttgcgacg
     1261 atgagcagag tgaagagcag atactccaga ccgtcgaaga actctatgaa gcgggtgaga
     1321 agaatatcta tgcccggctg ctgcatgtca tgtgccattt gcgtctcgcc cctgcagagg
     1381 cacgcaagtc ctggcaggca attctcaggc accgtgatct gatcgaatca aagcttgggc
     1441 gcaagaccgg cttccgggtg gcaatgctcg actactttgt aaacgaaagc cgcgatatca
     1501 agaacccgaa ggtaatcgaa attcacgtct acgctgagac ggcgagacag gccgcgatcg
     1561 acgaattgac cggcgtcttc aaccggcgct tctttgacgg ggcaatcgag cgtgaatgca
     1621 aacaggcgca gcgtcataag cgtgaatttt cattgctgat tctcgatatc gacaacttca
     1681 agcgcattaa cgacacgcac gggcatacga ccggcgacgc ggtcatctcg gcgctgggca
     1741 agttgctcaa aaaatccatt cgtggcgaag acacggcctg ccgtatcggc ggtgaagagt
     1801 ttgccgtcat cctgcccgaa acagggccag agggtgccga agttgtcagc gaaaagatac
     1861 gcagcgaatt tgcaaagctc aagattgctt cgaccgagct gagcctgagc gggggccttg
     1921 ccacctttcc gctcgacgcg caatctaccg aagacctgat cgaccgggca gaccagatgc
     1981 tctattttgc caagtattcg ggtaagaacc gcgtcatctg ctactcgcgc aacaagcgac
     2041 gtcatatccg ctatcctctc gagtgggagc tttcgatttc acgtcccttg ggagagcagg
     2101 gcaagaccct gtcgcaggat gtttcgattg aaggtatctc ttttgagctc gatgacgccg
     2161 cacaccccgg cgacctgatg agcctgcacc tgaatgtcga cgccgaaccg atagagctgc
     2221 cggtacagat tgtctgggtc gacaacaatt cacagaatcg ccggttcagg gcaggggccc
     2281 ggtttatccg cctgaccgag aagaacctcc acagcctgaa gcagaagcta ccccgccatg
     2341 gggtagttta gcaggttctg gctccttttc agcagcctgt taggctcccg ccttgttctg
     2401 tctcattttt tgcttgaagc aggggcgaag gtgtccgata tacaagtggg tgtcccgtgc
     2461 gacggggttg ttccggcaac ctgtgtctgc gcgttatgcc cgttaaagag aggaaaatgc
     2521 gctcatttaa gcatataaag acgttgccat tcaaggcgct gctctttgtt attatcctcg
     2581 gttcgccggc cgtgcaggcc gccgcgccgg accaggggtt tgtcgaaaaa atcctccggg
     2641 aaaaccgcta tttcatcgaa tttgccaacg tcagcgtctc gaattttggc tccccggaga
     2701 atgaaaaatc gctcaagcag gcaaaccagc acaattttaa cgcgaacctc tactacctgg
     2761 aaagcaacta cgttgatgcc tttaaggaaa tccgtcttag ccaggagatc ctgctcgacc
     2821 tgttctatga tgtactgcag aaacgttata tagaagattc gcgcacgctg ctcgacctca
     2881 acgcgccgat cattatccag tcgaaagacc ggaagtcagc gcattttctg cagctgggtt
     2941 accgcgatgt cgaagtggcg cgccagttcc gcgacatggg ttacaactat aaccgctttc
     3001 ttttttcgaa caagatccgg tactacatcg atggtatcaa acgcgcgcgt cgagcgaaac
     3061 gttttgcctt tttgtcgctg atcgaaagta aaacaccgat tgaagacaag gttgattacc
     3121 agacccagac gtgggacgat gcgcgcaaca aagatgaccg cgagaatatc cgcgattatg
     3181 agcgcgtgaa gaataacctg gttaaccaga tgaaccgcaa gctgtatagc gacgcgatga
     3241 acttctttct gcaccacgat gataactatg gtctcatctc aggccagaaa aagtccctgc
     3301 tgaaagattc tttcaaagaa ctgcagacga aacgccctga aaagcgtgcg cgtgatcctg
     3361 aatttcctgc aaaagatcag cccgaggtaa aaaaggcgga gccggccaag accgaacctg
     3421 cccctgccaa gaaataaccg tgaccagagc acgttacgtg tttacggtgg cggcattagc
     3481 cgccctcttt atgcccatcg ccgccacctt tgccgggccg ctggatatcc agaagctgcg
     3541 gctgaagggc gcacagaaca gcaaagaact caggcatttt cttgagacgg ggttggttgc
     3601 acagcggcag accccgattg aaatgcagat agaaaacttt gagcgcagcc ttaccgcgta
     3661 caaagaagcc aaacataccg aacggaacgc tgctttgcgc caggtgcaga acgtctacga
     3721 aaagttccag gtagagttcc aggcagtggc agatatactg cgagcgctgg tagacaaaca
     3781 ccaggctgcc tacaacgagc gcagcagcga agcgtttgcg aatacggcgt tacgcgaaaa
     3841 agttctctca acggtagagg tcggtaaaca ggaagaagtg cgtgcactgg ccgcgtacaa
     3901 tagccgcaat ttctcttaca gcgcgcacct gtacctgcgc tcactgcggc attatacgaa
     3961 cgctttcgaa ctgcgcggct ggcaacccct ggtgattatg ccggcaaagc cgggcaagaa
     4021 gccgaaggct gaaaaaacag cgccggcagc gccgcgctaa gcatcacggc gcagtaggtg
     4081 gcttcaccgc gacggccgtc acgggctcca ccactggttg cgcgacagta cccttgatat
     4141 taaattcaat acggccgtcg tcctgcagca cgttggcaaa cttcagcagc gttttcgggt
     4201 ctttgtcctg gtatttctgg tagaattctt ctgtcggttt aatgaccagg ttcagattga
     4261 gctccgtctg tgggcctaaa ttcagcgagc cgcgaatctg cgcgttgacc agccggccca
     4321 tagcctcgcc tgtttcaatc gctacctggt tctgctgtgc gcgggctttg agcacgatct
     4381 tgagaaaggg caggacgatt tcttctttca ggaaacgatt ttctttgtag gtgacctgcg
     4441 ccgattcggc ttctatactt actgtgccgt tgagcagctt gcctgtaccg ccggattttg
     4501 tctgttcgag caggctcttg atttcgagcg aatctatttt cagcgcaaaa tcgcccttgg
     4561 caaacgagag tatgacggct tcggtcgtca gatcgtattt gtcgcccagc aaagccgaaa
     4621 gcagggccac gcgtcccttg gcttcggcaa ttttcaggtt gccctgcttg tccccgtcac
     4681 ccgcgagagg tattttcagg ctttcaacct tgtagcggcc gaaggcagaa atcgacagat
     4741 cgccgacctc aacatggtat ccagaagctg cgagctggcg cagcgccagg attgtataag
     4801 cctcgagcgg ggcaaaccag acgaggccga ccaggaaggc cgcgaggcct gcaagaacgg
     4861 gagcaaaacg cttgagggtg ctctgccagg tgcgctcggg ttcgggcggc ggcgccggcg
     4921 tctgtaaatc ttcagtgttt tcgttttcgg tcattgcttt ctctggaagc cggaaatctg
     4981 cacagtgaag tcgtagatgc cgggtttctt gagcaccggc cggtagctga aactttctac
     5041 cttgtaaagc gtctgcacgc tctgttctac ctgccggata aagtcgagca ccaggttcgc
     5101 agggctctct ttgatcgaga ccttaacctc gcgcttcagg tatttttcct gcacgatggt
     5161 atcggtgggg gtcaggctgc ccactttttc ccgcagcccg atattcttga gcatggtttc
     5221 aatactcgaa agcatgttgt cgagctgctg cgagtcgccg gttttcatcg cctgcagtac
     5281 ctggaactgc cggcccaggt tctcgatctg cacagtgtcg cgctctgcgg agtcagtgcg
     5341 ttcgcgcagg tctgtcatgt aagcggcgaa gctccgcccg gcaaaaaata ccccgagaaa
     5401 aagcgcaaaa accccgagta tcatcaggta ggtgcgctcg cgcgggttga gtgattccca
     5461 gaattttttc atgtactact gcgtctggcc ttcggtcttt ttaactttgc agaacagcgt
     5521 aaacttgata cggttctgcc cgccgggccc cggtgtggag ttttcattct tcgattcgac
     5581 tgcagtcagc agcgatgctt tgccgagcga gcgtttgata tcttcgagtt tggcaaaatc
     5641 aggcactagc ccgttgattt ctacgccgcg gtcgctgtaa taaaacgacg tgatctcgag
     5701 cccggcttcg gtagaaatat tctggtgcag ctgcacgagt acggctgaaa gtcttggtgc
     5761 cttgagaaat acctcgataa tctcggtctt ctgtttttcc ttgcgcacga gctccgctgc
     5821 ataactcagt gcagaaacat tgtcaggcag ctgcgtgccc ggaaaaaact gctggaagac
     5881 cttcgcctgg cggtcttccc actctttgag ttgctggctg cggcggcgca ggtcgataaa
     5941 aaatgacagg ctgaaaatca ccacagcagc tgcagccagt gcgagcacga tgcgcgacgc
     6001 gcctgcctgc gagctgacca cccacttgcg cagctcgtct ttcagcaggt tcatttttcc
     6061 tgagaccgtc tgcgaatacg cgccacccat cgtcacaagg tgttcgcggt cgagcagtgc
     6121 cagaggggta cgttcgtagg gaaatggaac tacttcgtgg ttgaggccgc gtgcgagcgc
     6181 gttctcaatt accatcgcat tgttgagatc agagctgagc gaaaccggca gctccgaggg
     6241 gttcagcggc ttgctctctg cattttcctg ttcgggtttg acctgggctg catcgccttc
     6301 ctggtcaatc tgcagattgc ggagcgaaac ctcgagctcg cggctgatca gctgcgtgaa
     6361 atcatggatc gagtcctgca gcgatttgaa acggtctttg ctaatcttgt gcgtcttctt
     6421 gaaggcctcg aggtcggggc ttgggtcgag cgaggcgggg agggcgagca gaatgcggtg
     6481 gatatccgcc acatcacttt tccatttgcc cttgaggcgc gcaatcagcc agtcataccc
     6541 gacgggcatc acgcgcgcaa agcgccattc gcctgccagc acaatcacga tgatcgacac
     6601 catgcgcgaa acgtgcagca tgatgcacga ctcgagcccg gtaaaaggat gcagccggta
     6661 gagcgcgtcg tgcggcgtgt agataccgag taccgtcacc tggcgctgcc tgaattcgtc
     6721 gaggtagcgt atcagcgtat ctttgcgcga gccggcaacg atggcccggg tcgactggtt
     6781 cgcgaggttg gtggtcaggt aactgtcgta gacgacgtcc tgcaggtcat gcggtataag
     6841 cggttcgagt tcaaacggca gtacctcgcg caccttgcgg cggtctgcga aaggaaagct
     6901 cagttcgcgc gtgatcaggc tttcgtatgg tacgttaaag tggacgccga tggcccccgg
     6961 gtagcgttcg gcgacaaatg ccgcgacctg cgccggaatg ctttccttgg ggttgagcac
     7021 catggtctcg gcctggaaaa acgaacgccc ggcgatgccg ctgcgaaagg cgaggccttt
     7081 tacttcgtag ttattgattt cgatgacgac gggcgtgcgg gccatggtta ctcggaataa
     7141 aataccaggc gtttcgcgtt cggatcaaga atacccatca cttgcgccgt ctgcgaatcc
     7201 atcgacgcat cggcaacaat cttatacagt tggttgtcgg tggtgacgcg gtcgagaatt
     7261 tcctggaata aagacaggtt accacgggtt tttatctgca tttcgggcag gctggcaatt
     7321 tcatcgcggc tcttgaatcg cccgttacgc ttgatgcgct cggcgatgat cttgcgggcg
     7381 gcagccgggg tcatgaactc cgaaagcgaa agaatcacat ggtagggcgc cgagttgatg
     7441 ttaatctggt cggtcgaggt atcggcattg tagacgaggt gtgtggtaat gttgttggcg
     7501 agaatgtagt cctcgggcgt gagcgtcgcc ttttcgaggt cgctcaggcc agcggttgcc
     7561 tggttctttt tcttgtcgag ggagcgcagg tcttcataga gcaggcgcgc gtcaaagcct
     7621 ggcacaaaaa ggagctcttc aatagtctgc atcctcccgt tcttgatgcg ccgtggcggc
     7681 attagtcttt catagtcgtc gcgctcagcg ccgcgcggca tcggcgtgtt attctcgtcg
     7741 atgtagtcga ccaccgcgtg ccacaggtcg acggggatac cgagcgcgac ggaaagccgc
     7801 tggaagtact cgaagtggcg ttcgtcggtc tggtcttcaa acatgcgcac gaggcggtta
     7861 acgttcagtt taccggtctc ttcgctcagt gacagcgtga cggtgccgcc accgagcgga
     7921 attgtaggcg caatagtgaa gataccgaat tcatagagcg aatcgagtgg aatgcgcgtc
     7981 agcagcaggt tgccggtttc gagcccgctc tgcgccagct gcagcgtgcg ctcccgcgaa
     8041 gcggtggtgg taaccgcgcg gtattcgttg agcgcaaacc ggacgtaaac gagtgccacc
     8101 gatgccgtca gggtcagtat cagggtggtt gtaatcagaa tgattgcccc gcgctgccgc
     8161 agggtaaagc gaagggagtt ttttttgcgc agcagggttt ggctcctact cggcgagata
     8221 tttttcgata cgcgaacgat gcgcctgttt aggaatacag ccgaaggcgg cgttaaagag
     8281 cgccaggcgc acctcttcaa agataaaagg ggccatcgca ggtttcatcg accgcgagag
     8341 ttcaaccgtg acaaccggaa ttttcttttc gcggccgtag gtgccgagcg acccgggagt
     8401 cgggtacccc acgccctgcc caagcgggta atcggaaagc tgtgcgaaag gttttgcgat
     8461 ctcttcatcg cctgggtaga acaataccac gcggaacggc tggtgcaggt tatagattac
     8521 atatggtggg tacttgcgca tgaggcgcat gaaagcctgc gtttcgacgg ccttcgggtt
     8581 ctcttcatcg ccgtcagacg aaggaaaatc gcggttgaga tcgacgcctg tcgccgacac
     8641 gcgctggttg cgcgcgtaac catcgggatt atagttggga atcacgataa tgcggtggtc
     8701 attgcggtaa tcgcggctca taagccggat ttcacgcagc aggtattcga gtacgcgttt
     8761 ggtattgcgc tcatcgccgt gctgtgcacc gatgatgtag atgttatcgg catcttcggc
     8821 tccgcccggt gccggttcaa cgatgtgcgc aatgagtgga cggttcttgg ccgaggtgcc
     8881 gtagcggtat gcgcgcggca caattttctt ttcgtcttca gcttcgacct tgcaggtcag
     8941 atgggttacg agcttccagt gcgcccctga tgaaaggtag ggccgctctg cggcgcgcgt
     9001 ggcgcacgca cagagcagca gcgcaaggaa aacccggttc acttcaggga atccctcagg
     9061 tctttcatga tgtttttctg catcgcttcg ttggtgccgc cgccgatcga aagcagaatc
     9121 gcatcacgca gcaatttctc taccgggtac tcgcgtgtat aaccataacc gccgaacacc
     9181 tggatcgctg cgcgagcgac aaattctgcc atctgcgtcg cagaaagttt cgccgccgcg
     9241 gctgaaaggc tctcgcgtga ttccgggttc acgcgcagtg cggtctcgta aaccagagcg
     9301 cggctggcgc gatagcgcgc gtaagattcg gcgatcagct gctgtatctg gccaaaatct
     9361 gcaaggtgct tgccgaacgc catgcggtcg acagacgcat aacgcaccat ttcgtcgatc
     9421 gcgcggcgcg cgatgccgag cgacattgcc gcgagcgtca ggcgttcgat ttccaggttg
     9481 cgcatcatgt gcttcagtgc gccgctttct ttgccgatca ggttctccgc ggggacttca
     9541 acgtcttcaa acaccagctg gcaggtgggc gattgtttca tgcccatctt gatttctttc
     9601 ttgcctacgc tgaaaccctt gaagtttttc tcaacgacaa agctcgaaat tgccgagcgg
     9661 ttaccttttt cggtgcgtgc atagacgatg aagtagctgc cgcagttacc gttggtaatc
     9721 cactgcttaa caccgttgag tatatagcga tcgccctttt tttcagccac ggttgtgagg
     9781 cccagcacgt cggtaccggc tgcaggttct gacatcgcca tacccgcgag cgctgaaccg
     9841 tcgagtacct gcgagaggta gcgttcacgc aggccgggtt gagcagaatg ataaaggtta
     9901 ttcacgaaca ggacttcgtg tgccaggtaa ctcagtgtaa aagcggcgtc gctgtagctg
     9961 agttcttcat gcacgatgac cgaggctgtg gcgtcgaggc cgtgaccgcc ggcttcggct
    10021 ggcacggtga taccaaacag gctgtattca ctgcccattt tcctgagcaa tgcttcgttc
    10081 agtatctctt tgtcgtcgta ttctttgacc tgcggctcca gcacgccctt ggccaggtcg
    10141 tgcgtcagct cgcgcagtga tttgtgatcg tcggtggggt taaacaggtc gaacatagcg
    10201 gtaaattacg caaatgcgtt cggcctgaaa agaggaaaca atccttttgg tcagtcatcg
    10261 cccaggtcga ctgtctcgag ctggtagtta ccggccccgt cggagtgcag aatgacgtag
    10321 cggatgccga taccatcgaa ctgctcgggg cgtccgccgc cggtaccgac gatgacgtta
    10381 cgtattgcat caacccagaa ttcctggtag gtgtggatat gcccggcaaa aacaatatcc
    10441 acacccttgt cgagcgccat gttcaggaaa cgcgccgcct ggaaacgcga gttgaagctg
    10501 ttgttgcgca taccgaattc atcgaacgga ggtacgtgca taaacatcat cttgagcgcc
    10561 gaaccggact ggttgagctc gctttcagcg tagcggtaga cctcgcggct gatgctctgg
    10621 tctgccgaat cgagtaccag gaaaaaatct cctttgtggg taaaagcgta gttggttgta
    10681 ccaaacagac cccggtagaa attcatgtac ccctcgcgaa agccctcgtg gttgccgtag
    10741 gcggtataca ccgggccggc aaatttatct gacagcgcca gcgcccagct gtactcgtcg
    10801 ctgtttggtt ggttcacgaa gtcaccgacc agcagaaaaa agtcgaggcc acgcgagcgc
    10861 gcgctttcgg caatgcggct ggcagtcgaa tcgcgaaagt gcaggtcgcc cacgacgcca
    10921 aagcggtagg caccgccgcc ggtgaagttg gcgacagtta cgttgcgtgt ttcgcctgca
    10981 gcgatagtga gcgcccagtt gacgatacct gctttttcgc cggctgtgat ggtgccgaag
    11041 ctcgctgtgg gcagcgtatc gtggcgcatg ttcttcagct taaaattcag ggtgacgggg
    11101 gagctattgc gcgagacgaa gctgacctcg agcgtgggtc tctgcgctct tgcttcgatc
    11161 acattgccga cgagtgcgac cggtgcaaac ttcacctgga tatccacatc gttcacggtg
    11221 atttttccca cgctctggtt caggtcaaag cgttcagcct gtgggttggc gaggcagccc
    11281 gccaaaggta aaagcatcac cagtgtgaag taaaggtggt tgttcgagcg gatttgttgt
    11341 ttcaccagtt aactcctgca agggcaaaca gccggtaacc gcggccgtgc tccagtgtga
    11401 tgcgggtaaa aaagttgccg agaaaaattt ccacctggtg gacaaaggta ccacccagga
    11461 tactcgctcc gccgagtatg ttgtccttgc gaaaattgaa ggcgctcgaa aactcggtga
    11521 agcgtgtcgc gcgaaagcgc acgccgtgca tcagcacgag gttcgcgaag tgtgcatctt
    11581 tgcggaagtc gggcgtgtcg cgcaggtgaa tgtactgccg gccgataccc acggtcgtga
    11641 agatgccgat gccgcgcagg ctctttgcat aataagaagt caggttctcc gggtagagcg
    11701 tgaattctgc cagcgcattc ag
//
LOCUS       DAOQJF010000061        11651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2782_length_11651_cov_6.468179, whole genome shotgun sequence.
ACCESSION   DAOQJF010000061 DAOQJF010000000
VERSION     DAOQJF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11651
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2782_length_11651_cov_6.468179"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLF85_04935"
     CDS             <1..284
                     /locus_tag="PLF85_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE19037.1"
                     /translation="KRVGLIAWFMAALISVSTLYMKQHFVADIAMGFVLAFSVYRFYF
                     KPRAYTTNDQSMLPEWMSLGVVAIYLGFVGSMYTLFALGVKLPLDKIVR"
     gene            327..1313
                     /locus_tag="PLF85_04940"
     CDS             327..1313
                     /locus_tag="PLF85_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19038.1"
                     /translation="MKQTAADKPVHSYQRKAAIVAGGIADYAEQVHVKPIENFDLNRT
                     IFNTLEGNLPRKVIQTRIAKDVNWKPDAARSIDAEYEKKSAQKKLPPIDPKLIAFMKD
                     ECDFDVEHADGSFFDHLFFCYEYSHFYYPQHSALVMLLHSILGTGTNTFAMPKEKIPL
                     LKPLMSDFDWRHVEAFPSVLRLLYGTDLLKVLENDPAKINNLKQIKFHRVIDNVPITM
                     SGEDFWIQLNYQVIHLTDFIPVSNWAAHSNDSSFILFRKLYKFLTDSGKLEAKISYEA
                     PPMFAPVKNEQLPATGWLLTLMPTALGEWMTAQSIRKFSEKIGHDLNYELVF"
     gene            complement(1258..1947)
                     /locus_tag="PLF85_04945"
     CDS             complement(1258..1947)
                     /locus_tag="PLF85_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNE19039.1"
                     /translation="MTDNKILPKVKTNRIVVGALLFLGLVPAAAGIYRLFELGTDATI
                     TGDNARFHTAPLPVVLHIVSSLVYSLVGAFQFSAGFRRHNLPWHRRAGRIIALAGIVS
                     ALSGLWMTLHYPAAANDGPTLFYVRLVVAPAMFFFLVMGVYAIMQRKIQVHGAWMLRA
                     YALGLGAGTQVFTHVGYMLLVATPNGLSRDAMMALGWLINAAVAEWLIRRAAKKPIRN
                     SDHAQSSLKTS"
     gene            2112..2672
                     /locus_tag="PLF85_04950"
     CDS             2112..2672
                     /locus_tag="PLF85_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE19040.1"
                     /translation="MAAAIEVFIEEGYDLASMDRIAERADASKRTVYNYFESKELLLQ
                     AVLQKVNDDCKVTTQITYDPKASVESQLLAFVDQKLANLNNPPWVGMMRVLFGILGRN
                     EKLTQQVIGSVHDPDGSVRLFLKAACADGKLRIDDFELASAIFGAMIGGGIWWPLVFF
                     GTPSYMPEAGFRKEIVRTFLARYGTK"
     gene            complement(2687..3814)
                     /locus_tag="PLF85_04955"
     CDS             complement(2687..3814)
                     /locus_tag="PLF85_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNE19041.1"
                     /translation="MASAATLETLGIGSEFQENLRQFWARNELKLDIPVIDLQHIWLV
                     YLIMELENENLGAANEAFPEEKIRKIMGDLLSFAAEHFSLEEDLLMAFDMPDAFEHAE
                     KHRHFVQFLSERANEVRTGKQDSVKVLISFLKDWLTMHIQKDDRHYATYCRDSGINLK
                     QYFQRQIEKHELSIDRGQVAVYKLVSESDEVHEIVNENITSNVVKIWNSNNLSTQIPI
                     IDLQHLWLIHMVVELDLASRSRNMVTSAREKIFQRVVQQATQYAQEHFSTEERIMEKF
                     RFAGLAGHLKQHQNFRDFVAKRANEFRQGDTQAAIKLVHDLREWLLSHIAIEDHKITI
                     AFKTHLNDIQKFVRDLVTSGEVTIRKKQIDLYNQVMGLQRV"
     gene            complement(3909..4313)
                     /locus_tag="PLF85_04960"
     CDS             complement(3909..4313)
                     /locus_tag="PLF85_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19042.1"
                     /translation="MRYSVWQVFSPDAVVRLSCELYRAIGGGDLHSRPRTEAWRDESL
                     LNLSAVYTVAHGFVLGVPGDGLTFQLLENWEKRSSGTELVGVSVRARSLGEPYLNLDY
                     RHGQLHCELPAGTVDADHFGRLVDIAKKDHTA"
     gene            4468..5100
                     /locus_tag="PLF85_04965"
     CDS             4468..5100
                     /locus_tag="PLF85_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE19043.1"
                     /translation="MSRESVHIGIVENDPEFRDSLLITLKKLLPKAKLSWWQSAEGLL
                     KEDRAGLPDVLLLDVMLPGMSGIDLARELAEDGFEGKIVILTNMNSDSLILEAIENGA
                     VGYILKSDLHELKSIMETVLAGGAILTPTIALRVMTTLRQRRLESPDFGLSARLQQVL
                     ELMVAGKTIPQVAKALRLSPSTVHGYVKDIYKKLNVHNRAQLVGKVRNFG"
     gene            5136..6992
                     /locus_tag="PLF85_04970"
     CDS             5136..6992
                     /locus_tag="PLF85_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE19044.1"
                     /translation="MALRLIARVSFAILLLAAVGVRAAETTPVVDLKTQSRVVLKNLP
                     WEFYWQKLLTPSDFSDASLKPDLVLPATTIWNRLTISGRQLGSFGFATYRLRFTVSGH
                     SERLGLRLPAPLSAFRLYINGEKLAEEGSLTTSPENFEARRRTSLHFFRLQDGNVEIV
                     IQVANFLLYKGGLRSEIELGHAPAIQKYGMRYFAIDLFGMGLIFSIMLYHFLLYFLSR
                     KDLAILIFAGLGLDYFILTTLFGEQTTSLFIPGLHMDIHTRLTSACTYLLPALVVEFT
                     GTLYPGTVPATTKRVFWLLAGMFILLLVLPPRVFLFYNVLYYMIVGGAGGAVSMWAVF
                     RAVRENRAGARLLAAGILVLMALTVYAVFLVASDSQAGSFLSLGFSIFALTQSGSLAH
                     AHAALTRENSAMQLSLERSRQALDSQRKQIEANLHDSLGGNLTDIKLGLEAIEKDPRA
                     ARLKPEIRRLDQRVGGTIASLRTQLLFLEDMQMAVNDFVSGINLILLRRYQMARRPVE
                     IRVSPESRAQSRNLIANRVISEENIPELCLIVQELCSNTLKYSAGTAEWEITATGTGL
                     QIRVNTKSRRAPSGSGRGRHTLRARTEAIGAKFEEVFSGRNYQASVEMRSSS"
     gene            complement(7002..10559)
                     /gene="dnaE"
                     /locus_tag="PLF85_04975"
     CDS             complement(7002..10559)
                     /gene="dnaE"
                     /locus_tag="PLF85_04975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNE19045.1"
                     /translation="MAENGSTSPATAMPATNGQAHDFAHLHLHSTYSLLDGAIRPSDL
                     VKKVKELGMNSVAVTDHGNMFAAIEFYEAAKKEGVKPIIGYEAYVAPGSRFEKRNQED
                     LVDGRAYHLILLAKNKQGYKNLTKLASKAYTEGLYYKPRIDYELLAQHSEGLVGLTAC
                     LAGEVNRLIYRQQMDKAAELSGRLNEIFGKGNFFLEVQNHGIPEQIEVAKGAAELSRK
                     LDIPLVLTNDSHFLTRKDQKAQEVMLRIQMNKKITDPLEFGFNDEFYVKSPAEMFKLY
                     PELPQAFYNTLQIAEMCNFDFEFGAPLLPDFQIPPGMSLGDYLISEAVTGLSRRFDGN
                     VSDKHRQRLQYELDVIKNMGFEGYFLIVADFINYAKRNGIPVGPGRGSAVGSLVAYSL
                     GITDLDPLKYDLLFERFLNPSRKEMPDIDVDFCRDRREEVIRYVVDKYGEDHVSQIIT
                     FGTLSAKAVIKDVARVLGVDFQLINALSKNLPDTPGISLKDAVAAAPEVQKYFEQGEK
                     ERQLYAIAQTLEGMPRNSGKHAAGVVIAPQPLDEIVPLAKDSKTDSIVTQYDMTLLPK
                     VGLVKMDFLGLKNLTVIQSCVAEIKRRHNIDLDMTRLPLDDLKTYQLLTQGKTRGIFQ
                     VESTGITKLLVQAKPRAFEDIVAIIALYRPGPLESGMTESYVKRKNGEMKLEYPHPSL
                     ELILKDTYGTFVYQEQIMLASQIIGGFTLAEADTLRKAMGKKNADEMAKMKAKFVSGA
                     VEKKFQEKWASELFDNMAEFAKYGFNKSHSAAYGLITYQTAYLKTNYTIEFLKASMDA
                     DIGDTDKIIGYINHAKELGIEVLPPDINESDVYFTILDDHRLRFGLLGIKGLGEAPAR
                     AIVEARAHLGGKFKSLAEFAESLDTKTLNRRLLDALAFAGATDSLGFTRAAIVQSADE
                     ILKFSAAAQLDKSSGQTSLFGGVDEEAARLQIQNAREFSDEEKLLKEKQVLGLYLTSH
                     PLDRFTDVAAHMRLTQIADLDDGVSKERRVSVLAVIDSLRTVTSKRGAFYLLKITDRT
                     GQIEVRIFQNMFEQVKPFIRENSCAIFDIRVQAFREEEVTTMNYSLAGIVPEEKFTER
                     VEKSLHLYLNVKTPSDLEAMIGRVKTTLRKYSGNNPVYLYWREIEGGPLQSIKAHSSF
                     CVKYDKALLKDVSQLLGAEKYALFKVGGIMEAAQGATVN"
     gene            10919..11095
                     /locus_tag="PLF85_04980"
     CDS             10919..11095
                     /locus_tag="PLF85_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19046.1"
                     /translation="MTTKDTAIETIRTLADDAGWEEIMYSLYLRQKVQKGVKDFEEGR
                     VVSHEDLKKKLLHS"
     gene            11092..11397
                     /locus_tag="PLF85_04985"
     CDS             11092..11397
                     /locus_tag="PLF85_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE19047.1"
                     /translation="MKEIKWSAEAAADLEGIKRYIAQGSPHYATIFIEKIFQAVERLV
                     QHPLSGRVVPEFEDQQYREIFHGNYRIIYTPLEDVVRIYAVVHGSRDLRNDDLNPNT"
BASE COUNT         2592 a         3175 c         3184 g         2700 t
ORIGIN      
        1 gcaagcgtgt cggccttata gcctggttta tggcggcgct gatttctgtc tctacgctct
       61 acatgaagca gcacttcgtc gccgatatcg cgatggggtt cgttctcgcc ttttcagtct
      121 accgcttcta cttcaagccg cgcgcctata caacaaacga ccagtcgatg ctacccgaat
      181 ggatgagcct gggtgtcgtg gcgatctatc tggggtttgt gggatcgatg tatacgctgt
      241 ttgcacttgg ggtgaaacta ccgctcgaca agattgtccg gtagggcttt ggttgacgcc
      301 gccgtaacag cagcgagtcg agctgtatga aacagacagc agcagacaaa cccgtccata
      361 gctaccagcg caaggctgcg atcgttgccg gcggcattgc cgactatgca gagcaggtac
      421 atgtcaagcc catcgaaaat tttgacctga accgcacgat cttcaacacg ctcgagggca
      481 atctgccgcg taaagtgata cagacgcgca tcgcgaaaga tgtcaactgg aaacccgacg
      541 ctgcgcgctc gatcgatgcc gagtacgaaa aaaaatccgc gcaaaagaag ctgccgccga
      601 tcgaccctaa gctcatcgcc ttcatgaaag acgagtgtga ctttgacgtc gagcatgctg
      661 atggttcgtt ttttgaccac ctgttcttct gctacgagta ctcgcacttc tactacccgc
      721 agcattcggc gctcgtgatg ctgctccact ctatcctcgg cactggcacc aataccttcg
      781 cgatgccgaa agaaaagatt ccgctgctga agccgctgat gagcgacttt gactggcggc
      841 acgtcgaggc atttccgtca gtactacgcc tgctctacgg taccgacctg ctcaaggtac
      901 tggagaatga cccggctaag atcaataacc tcaagcagat caaatttcac cgcgtgatcg
      961 acaatgtgcc gatcaccatg tcgggcgagg acttctggat ccagctcaac taccaggtca
     1021 ttcacctcac ggattttatt cccgtgagca actgggcagc gcactcgaat gattcgtcct
     1081 tcattctttt cagaaaactg tacaagtttc tgaccgacag cggtaaactg gaagcaaaaa
     1141 tcagctacga agcgccgccc atgtttgcgc cggtgaagaa cgaacagctg cccgcaacag
     1201 gctggttact gacgctcatg cctaccgcac tcggcgaatg gatgaccgcg cagtcgatca
     1261 ggaagttttc agagaagatt gggcatgatc tgaattacga attggttttt tagctgcccg
     1321 cctgatcagc cattcggcga ctgcggcatt gatcagccaa cccaacgcca tcatcgcgtc
     1381 gcggctcagg ccgttgggcg tggcgacgag cagcatgtaa ccgacgtgcg tgaaaacctg
     1441 tgtgcctgca ccgaggccca acgcgtaggc gcgcaacatc caggcgccgt ggacctggat
     1501 ttttcgctgc ataatcgcat agacccccat gaccaggaag aagaacatgg ccggtgcgac
     1561 gacaagccgc acgtagaaga gcgtcggtcc gtcgtttgcc gccgccgggt agtgcagcgt
     1621 catccacagg ccagagagag ccgacacaat acccgcaagc gctatgatgc gccctgctct
     1681 tctgtgccat ggcaggttgt gccggcgaaa gcccgctgaa aactggaacg caccaacgag
     1741 cgaatagacc agagaactca cgatgtgcag caccactggc aatggcgcgg tatgaaaacg
     1801 cgcattgtcg ccggtgatgg ttgcatccgt acccagttca aacaggcggt aaatgcctgc
     1861 cgcagcgggc acgagcccga ggaaaaggag agctcctact acgattctat tggtcttaac
     1921 ttttggtagg attttgttgt cggtcatagt tgctggaaca aaggttgctc cggcttgtaa
     1981 actacaccgt acagtgtatc acgtcaagta atttacactg atgagtgtaa atttggtgga
     2041 cggggcagcc aacggtgact gtgcgttacc tgatggagaa ggttgccgga tcaagaaagc
     2101 gcgactcgat tttggcagcc gcaatcgagg tttttatcga agagggttat gacctcgcca
     2161 gcatggatcg catcgccgaa cgcgccgatg cgtcgaagcg cacggtttac aattatttcg
     2221 agagcaaaga gctactgttg caggctgtac tgcagaaggt taacgacgac tgtaaggtga
     2281 cgacccaaat tacctatgac ccaaaggcaa gcgtcgaatc acagctgctc gcgttcgtag
     2341 accagaagct tgcgaatctt aacaaccctc cgtgggtcgg catgatgcgc gtactctttg
     2401 gtattctggg gcgaaacgag aagctgacgc agcaggtaat cggcagcgtg cacgaccctg
     2461 atggaagcgt cagactgttt ctgaaggcag cgtgcgcaga tggaaagctc agaatcgacg
     2521 attttgagct ggcttcggcg atattcggcg cgatgatcgg cggcggcatc tggtggccgc
     2581 tagtcttctt tgggacgccg agttacatgc ctgaggccgg attcaggaaa gagattgtgc
     2641 gcaccttcct ggcgcgttat ggaacgaagt gattgctacc aggaattcaa acccgctgta
     2701 agcccatcac ctggttatag agatcaatct gcttcttgcg gatggtgacc tcgccgctgg
     2761 ttaccaggtc gcgcacgaat ttctggatat cattcaggtg ggtcttgaac gcaatagtaa
     2821 tcttgtggtc ttcgatcgca atgtgcgaga gtaaccattc gcgcaggtcg tgcaccagct
     2881 tgatggcggc ctgcgtatcg ccttgccgaa actcgttcgc gcgcttcgct acaaagtccc
     2941 gaaaattctg gtgctgcttc aggtgcccgg ctagtcctgc aaaacgaaat ttttccatta
     3001 tacgttcttc ggtagaaaag tgctcttgcg cgtactgagt agcctgctgt actacacgct
     3061 ggaatatttt ttcgcgcgcg ctggtgacca tgttgcggct gcggctggca agatcgagct
     3121 ccactaccat gtgaatcagc cagaggtgct gcaggtctat aataggtatc tgggtgctga
     3181 ggttattgga attccagatt ttcacaacat tgctggtaat gttttcgttc acgatctcat
     3241 ggacttcgtc cgattcagag accagcttat agacagcgac ctgcccacga tcgatactca
     3301 gttcatgctt ttcgatctgc cgctggaaat attgcttcag gttaatgcca ctgtcacggc
     3361 agtatgtcgc gtaatggcgg tcgtctttct gtatatgcat cgtgagccag tctttcaaaa
     3421 atgagataag cactttgact gaatcctgct tgccggtgcg cacttcattg gcccgttctg
     3481 agagaaactg cacaaagtgc cggtgcttct ctgcatgttc aaaagcgtcg ggcatgtcga
     3541 aggccataag cagatcttct tcaagactaa agtgttcggc agcgaagctg agcaaatcgc
     3601 ccataatctt gcgtatcttc tcttctggaa acgcctcgtt cgccgcgccc aggttctcat
     3661 tctcgagctc cataatcagg taaaccagcc aaatatgctg caggtcaata accgggatat
     3721 ccagcttcag ctcgttcctg gcccagaatt gcctcaggtt ctcttggaat tcggagccta
     3781 tacccaaagt ctcgagtgtt gctgctgatg ccatggccca gattatcggc agcgacatcc
     3841 tgtcgatcta ttaatagatt tattggtctt ctattctgaa tattgattgg attttttcaa
     3901 agaggccgct aggcagtgtg gtcctttttt gctatgtcga ccaggcggcc aaaatggtcg
     3961 gcatcgactg ttcctgccgg caattcgcaa tgcagctggc cgtggcggta atcgaggttc
     4021 aggtacggtt cacccaacga tcgagcacgc acactcactc ccactaattc ggtgccactg
     4081 ctgcgctttt cccagttctc gagcagctgg aaggttaaac catcccccgg gaccccgagc
     4141 acaaagccgt gggcaacggt atagacggcg gacagattga gcaggctctc atcgcgccag
     4201 gcttcagtgc gtggcctgct gtgcagatcg ccgccgccaa tagcgcggta caattcgcac
     4261 gacaggcgta cgaccgcatc gggcgagaaa acctgccata cactgtaccg catgttacca
     4321 attgccacga cgctatggta atgcgacttc tttacgcgct tgtcaccccc ctgttcaggg
     4381 ggttacaccc ctaaacaagg ggggtaagag gtatagattc tggtggtgca tctacagcct
     4441 tttcggcgcg tgagcctggg tgatagcgtg agcagagaga gtgtacacat cggcatcgtc
     4501 gagaacgacc ccgagtttcg tgatagccta ctgataacac tcaagaagct gctaccaaaa
     4561 gctaagctca gctggtggca aagcgccgaa ggactgctga aagaagacag ggctgggctg
     4621 ccggatgtcc tcttgctcga tgtgatgctg ccgggaatgt caggcatcga cctggcgcgt
     4681 gaactggctg aggacggctt tgaaggcaag atcgtgattc tcaccaacat gaactccgac
     4741 agcctgattc tcgaagcgat tgaaaacggc gctgtaggat acatactcaa atcagatctg
     4801 cacgaactga aatctataat ggaaacagta ctcgcgggag gcgcgatact gacaccgacg
     4861 atcgccctgc gcgtcatgac aacgctgcgt cagcgcagac tagaatcccc tgattttggt
     4921 ctctcggcgc gcctgcagca ggttcttgaa cttatggtag ccggcaagac tataccccag
     4981 gtcgcaaagg cgctgcggct gagtccttcg acggtgcacg gctacgtgaa ggatatctac
     5041 aagaaactga atgttcacaa ccgggcacaa ctggtcggca aagtaagaaa tttcggttga
     5101 aaggcgcaga accgaagtca atccgccacg tgcgaatggc gctgcgtttg attgcccgtg
     5161 tttcgttcgc gattcttttg ctggctgcag tgggcgtgcg cgcagcagag accaccccgg
     5221 ttgttgatct gaagacgcaa tcccgcgtag tactcaaaaa cctgccgtgg gaattctact
     5281 ggcagaaact gctcacgcca tcggatttca gcgatgcatc cctcaaaccg gacctggttc
     5341 ttccggccac aacaatctgg aacaggttaa caatctctgg tcggcaactc ggctcgtttg
     5401 gtttcgcaac ctatcggcta cgattcactg tgtcaggcca cagcgagcga ctggggttgc
     5461 gcctgccagc cccgctctcg gcctttcgcc tctatatcaa cggcgaaaag ctggcggaag
     5521 agggaagcct caccacctcg ccggaaaatt ttgaagcgag gcgacgcacc tctctgcatt
     5581 ttttccgact gcaggatggc aatgttgaga ttgtgatcca ggttgcgaat tttcttctct
     5641 acaaaggcgg gctgcgcagt gaaatcgaac tcggccatgc tccggcaatt cagaagtatg
     5701 gtatgcgcta ttttgctatc gatctgttcg gcatggggct catcttttcg atcatgctgt
     5761 accattttct gctttacttt ctttcgcgaa aagacctggc gatcctgatt tttgccggtc
     5821 tgggtctgga ttattttatc cttacaactc ttttcggtga gcagacaacg agcctgttta
     5881 tccccggcct gcatatggat atacacacac ggctgacgtc ggcatgtaca tacctgctgc
     5941 ccgctctcgt tgtggagttc acaggtactc tctacccggg cacagtgccc gcgacgacca
     6001 agcgtgtttt ctggctgttg gcaggcatgt tcattctgtt gcttgtgctg ccccccaggg
     6061 tgttcctctt ctacaacgta ctctactaca tgattgttgg gggtgcgggt ggcgctgtga
     6121 gcatgtgggc cgtcttcagg gccgtgcgtg aaaatcgtgc cggtgcacgc ttactcgctg
     6181 ccggtatcct tgttctgatg gcgctgacgg tttacgcagt attcctcgtc gcatcagact
     6241 cgcaggcagg cagttttctc tctttgggat tttccatctt tgcactcact cagtctggtt
     6301 cattggcgca tgcacacgcg gcgctcaccc gcgagaactc agccatgcaa ttatccctcg
     6361 aacgcagccg ccaggcgctc gacagccagc gcaaacaaat cgaggcaaac ctgcacgaca
     6421 gtctgggcgg taacctcacg gatatcaaac tgggcctcga ggcaatcgaa aaagatccgc
     6481 gcgcggcgcg cctcaagcca gaaatccgtc gactcgacca acgcgtcggg ggcactatcg
     6541 cctcgctgcg cactcagctc ctctttctcg aagacatgca gatggcggta aatgattttg
     6601 tttcgggtat taacctgata ctgctacgcc gctaccagat ggccaggcgc ccggtcgaaa
     6661 ttcgtgtctc gcccgaaagc cgtgcgcaga gccgtaacct gatcgccaac cgcgtgatca
     6721 gcgaagagaa tattcccgag ctgtgcctga tcgtccagga gctctgcagc aatacactga
     6781 agtattcggc cggcacagcc gagtgggaaa tcaccgcgac gggcaccggg ctgcagatac
     6841 gcgtgaatac aaagagtcgg cgcgcgccat caggtagtgg acggggaaga catacactgc
     6901 gcgcacgcac cgaggccatc ggggcgaaat ttgaggaagt attttccggg cgaaattacc
     6961 aggccagcgt cgagatgaga tccagctcgt agcaggaccc gctaatttac cgtcgcgccc
     7021 tgtgcggctt ccatgatacc gccgaccttg aagagcgcgt atttctctgc gcctagcagc
     7081 tggctgacat ctttcagcag cgctttgtca tatttcacgc aaaagctgga gtgtgccttg
     7141 atcgactgca gtggtccacc ttcaatctca cgccagtaaa ggtacacggg gttatttcct
     7201 gaatacttgc gcaacgtcgt cttcacacgg ccgatcattg cctcgagatc agagggagtt
     7261 ttgacgttca ggtacaggtg cagcgatttc tcgactcgct ccgtgaattt ctcttcgggc
     7321 acgatacccg cgagtgaata gttcatcgtc gtgacttctt cttcgcggaa agcctgcacg
     7381 cgaatatcaa aaatggcgca cgaattttcg cggatgaagg gcttcacctg ctcgaacatg
     7441 ttctggaaaa tacggacttc aatctggccg gtcctgtcgg tgatcttcag caggtaaaag
     7501 gctccgcgct tcgaggtgac cgtacgcaac gaatcgatca cggcgagaac agagacgcgc
     7561 cgctctttcg aaactccgtc gtcgaggtcg gcaatctgtg tcaggcgcat gtgcgcggcg
     7621 acgtcggtga agcggtcgag agggtgcgat gtcaggtaga gaccgagcac ctgcttttct
     7681 ttgagcagct tctcttcgtc gctgaactct ctggcattct gtatctgcag ccgtgcagcc
     7741 tcttcatcga cgccgccaaa cagcgaagtc tggcccgacg atttgtcgag ctgcgccgcg
     7801 gctgagaatt tgagaatttc gtctgccgat tgtactatag ccgcgcgcgt gaagccaagc
     7861 gaatcggtgg caccggcaaa ggcgagcgca tcaagcagcc ggcggttgag ggttttagta
     7921 tcgagcgatt cggcaaactc ggccagcgat ttgaattttc cgcctagatg tgcgcgtgct
     7981 tcaacaatcg cgcgcgcagg cgcttcgccc aggcccttga tacccagcag gccgaagcgc
     8041 aggcggtggt cgtcgagaat ggtgaagtaa acgtcggact cgttgatatc cggcggcagt
     8101 acctctatgc ccagttcttt cgcgtggttg atgtagccga tgatcttatc ggtatcgccg
     8161 atatcggcgt ccatcgaggc cttcaaaaac tcgatagtat agttcgtttt gaggtatgcc
     8221 gtctggtagg tgattaatcc gtaagcggcg gagtgtgact tgttgaagcc gtatttcgca
     8281 aattcggcca tgttgtcgaa aagttccgag gcccattttt cctggaattt cttttcgaca
     8341 gcacctgaaa caaacttggc cttcatcttg gccatctcgt cggcgttctt cttacccatc
     8401 gccttgcgca gcgtatccgc ttcggcgagg gtgaacccgc cgatgatctg cgacgcgagc
     8461 atgatctgct cctggtagac aaaagtaccg taagtgtctt tcaggatcag ctcgagcgac
     8521 gggtgcgggt actcgagctt catctcgccg ttcttgcgct tcacataact ttcggtcatg
     8581 cccgactcaa gtgggcccgg acggtagagg gcgatgatcg caacgatatc ttcaaacgca
     8641 cgcggctttg cctgcaccag cagcttggta ataccggtcg attcaacctg gaaaataccg
     8701 cgcgtcttgc cctgcgtgag cagctggtaa gttttcaaat cgtcgagcgg cagccgcgtc
     8761 atgtcgaggt cgatattgtg ccggcgtttg atctcggcga cgcatgactg aatgaccgtg
     8821 aggttcttca gaccgagaaa gtccatcttg acgaggccga ctttcggcag cagcgtcatg
     8881 tcgtactgcg tcacgatcga atcggtcttc gaatctttcg ccaaaggtac aatttcgtcg
     8941 agcggctgtg gagcgatcac aacaccggca gcatgtttgc ccgagttgcg cggcatgcct
     9001 tcgagagtct gcgcaatcgc atacagctgg cgttcttttt caccctgctc aaagtacttc
     9061 tgcacctcgg gtgcggcagc gaccgcgtcc ttgagcgaga tacctggtgt atcgggcagg
     9121 ttcttcgaca gtgcattgat cagctggaag tcaacaccca gtacgcgtgc gacgtctttg
     9181 atgacggcct ttgccgagag agtaccaaag gtgataatct gcgagacatg gtcttcgccg
     9241 tatttatcga cgacgtagcg tatcacttct tcgcggcggt cgcggcagaa gtcgacgtcg
     9301 atatcgggca tctctttacg cgacgggttc aggaagcgtt cgaagagcag gtcgtatttc
     9361 agcggatcaa ggtcggtgat accgagcgag tacgcaacga gcgaacccac cgctgaacca
     9421 cgacccggcc ccaccggtat accattgcgt ttcgcgtaat taatgaagtc ggcaacgatc
     9481 aaaaagtagc cctcgaagcc catgttcttg ataacatcga gttcgtattg caggcgctgg
     9541 cggtgtttgt ccgagacgtt gccgtcgaag cggcgcgaga ggccggtgac ggcttcggat
     9601 ataaggtagt cgcccagcga cataccgggc ggaatctgga aatcgggcaa gagcggcgcg
     9661 ccgaactcaa aatcgaagtt gcacatctct gcgatctgca gcgtgttgta aaaagcctgc
     9721 ggcagctcgg ggtagagctt aaacatctct gccggtgatt tcacatagaa ttcgtcgtta
     9781 aagccaaact ccagaggatc agtgatcttc ttgttcatct ggatacgcag cataacttcc
     9841 tgcgctttct ggtctttgcg cgtcaggaag tgcgagtcat tggtcaagac gagcggtatg
     9901 tccagtttcc gggaaagctc cgccgcgcct ttggcgacct cgatctgctc gggaataccg
     9961 tggttctgta cctcgaggaa aaaattgccc ttaccgaaaa tttcgttcag ccggcccgaa
    10021 agttcggccg ccttgtccat ctgctggcgg tagatcagtc gattgacctc gcccgcgaga
    10081 caggccgtga gacccacgag gccttcgctg tgctgtgcca gcaattcgta gtcgatgcgg
    10141 ggcttatagt agagcccttc ggtgtaagct tttgaagcga gcttggtgag gttcttgtat
    10201 ccctgcttgt tctttgccag caggatcagg tggtacgcgc ggccgtcgac caggtcttcc
    10261 tggttgcgct tctcgaatcg gctgcctggc gcgacgtagg cctcgtagcc gatgatgggt
    10321 ttcacaccct cttttttcgc cgcctcgtaa aattcaatcg cggcgaacat attgccgtgg
    10381 tcggtaacgg ccaccgagtt catgccgagc tccttcacct ttttgacgag gtctgaaggg
    10441 cgaatggcgc catcgagcag cgaatacgtc gagtgcaggt gcaggtgtgc aaaatcatgc
    10501 gcctgcccgt tggtcgcggg catggcggta gcgggggatg tactgccgtt ctcagccatt
    10561 ctttctattc aatgaatcgg cacctgtgca atgggtcgat aagccataga agaagaaacc
    10621 ctctacaact tattgacgtg tgcgaaacgg ggaaaccccg ggggagcctg ctgcgtgtct
    10681 cgcagcatgg gacataacgt cagttaaaaa acgggaatag ctttgctgca ggcgcactag
    10741 gctcacgagg gcaaatcaga aaagaatgtt gtgcgccgca ccacagaata tccctgctcg
    10801 gctttgagca gatcattagt ccctttgctg cctaagagct atgccagcaa gaatggatcg
    10861 ccatgaaata accttgctcg cgtgagcagt tcggtatatc ttggcgctgt acctaactat
    10921 gacgaccaaa gataccgcca tcgaaacaat aagaaccctc gccgacgatg ccggttggga
    10981 agagatcatg tactccctct acttgcgcca gaaagtccag aagggtgtta aggatttcga
    11041 agaaggcagg gtcgtttctc acgaagacct gaagaaaaag cttctccatt catgaaagaa
    11101 atcaaatggt cggccgaagc cgccgccgat ctcgagggca tcaaaagata cattgcccag
    11161 ggctcgccgc actatgcaac aatcttcatt gaaaagatat ttcaagccgt cgaacgtctt
    11221 gtgcagcacc cactttcagg ccgcgttgtc cctgaatttg aagaccagca gtaccgggag
    11281 attttccacg gaaattaccg catcatttat acaccacttg aagacgttgt acggatttac
    11341 gccgtcgtac atggcagccg cgatctacgc aatgacgatc tgaacccaaa cacctaaccc
    11401 cccgcgcact cccgcaggct tctccccccg gccaaaatga ctctttaatt gcggggctct
    11461 tagggaaagg ggggcgtgtg ctggttcgcg tttcgcctcg aaacgctcac agcaaaaaaa
    11521 cgcgccacgc gctttcctgc ggtgccgccg cgccttcctc catgaagtca cgccgtcacc
    11581 gctccggcaa acctccgtgt ttgccgggtg aatataaagc ctgcctgccc acccgccacc
    11641 gtgcgcgggg g
//
LOCUS       DAOQJF010000062        11640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2788_length_11640_cov_5.014502, whole genome shotgun sequence.
ACCESSION   DAOQJF010000062 DAOQJF010000000
VERSION     DAOQJF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_11640_cov_5.014502"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLF85_04990"
     CDS             <1..859
                     /locus_tag="PLF85_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNE19048.1"
                     /translation="GIMTFISLATFIGLLAVLGRWQVSGEGMRLSLRFRFLNNLSTGA
                     PVRISGGLPVGYVENIYEKDLRTYVQISLRKDLRDKIPKRAETVFAIYTTGLMGQKYI
                     NITVPDPKPEDTFFKDGDEWVGIDPPSVDQMMMAFSSWFDGKNGGQVLAEIMKETETF
                     LANLNGIVNENRGDIRQTIRQARESFTSLSAQLDTLMQKLNILSKNFTDISNQNKQDI
                     QIMLENMSKISRDLNLITQRVNSGRGSVGKLIQDEELYKNANEAAYHARELFKKLEEN
                     PSLFLNKSN"
     gene            861..1778
                     /locus_tag="PLF85_04995"
     CDS             861..1778
                     /locus_tag="PLF85_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNE19049.1"
                     /translation="MALPLDATRRASVRAGSFVLLGFLVLILSAIIGARIHTARAGFT
                     IDITFSFLSNLTRNAKVLLSGGRQVGYVQDIFQKDQQTYVRLYLENSLRGKMPNTSDT
                     QISIFSNNLMGQKYINIQFHDLKPNQPVIEPGQVVRGISPPSFEQMMLSFSSWFEGRS
                     AGEVAELIFAKAAMLRSNIDAVMNENREDLAATMSGAKNYFTAVSDQFESLKSNISLI
                     ARNSEEILTAQQQSLTQMVSNSAAMAQNLEALEKALSNNRGSLGKFNKESKVLRENVR
                     QTIEYSRSFIKCIQERPWVIIYKESCRER"
     gene            complement(1805..2374)
                     /locus_tag="PLF85_05000"
     CDS             complement(1805..2374)
                     /locus_tag="PLF85_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNE19050.1"
                     /translation="MKRFLSTGLILLSLATANSGQLVAQSAHSRLTDTTLLKRFDSLS
                     RKLMCTCGCNMPLRNCNHTGHCNAWPQRDALDKLLMAGYTDDQILNGFRNGFGKNAES
                     AEFFAMARTPDYGYMAAQFRDGFGSQILSIPESNYLGIFSLFGFILCAGVVMLFIRGR
                     RKKPGTETAQLLDEKHREELLRRISAEEN"
     gene            2355..3104
                     /locus_tag="PLF85_05005"
     CDS             2355..3104
                     /locus_tag="PLF85_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19051.1"
                     /translation="MDKKRFTTINIMKFTCRIKIIFVVLLMSSVPLWASDWETGLTGA
                     WSVASRQLTASTAYTAGSEDSRFIDFGGRVAHRLGPGFLGVHFGLYSSFGLREQVTAS
                     NAHATDFVVNKSAETGLPVSFDSGTYRLALGWNALQLRYRYYLTEYLFAEAGAGAAYG
                     FGGIEAAFKGSNSAGSVSSTYYHRYSEWGFISSASAGISLPLGDTIRLEAACEFAWLW
                     AQIREPNIFRTGDFTLSQTFFRPMVGIVLKF"
     gene            complement(3185..4627)
                     /gene="ahcY"
                     /locus_tag="PLF85_05010"
     CDS             complement(3185..4627)
                     /gene="ahcY"
                     /locus_tag="PLF85_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNE19052.1"
                     /translation="MKTAETPAGVAIGQLPYKVADINLAEFGRKELDIAEHEMPGLMA
                     LRKKYGAEKPLKGAKVMGSLHMTIQTGVLIETLKELGADVRWCSCNIFSTQDHAAAAI
                     AKSGTPVFAWKGETLEEYWWCTEEALTWPDGSGPDLIVDDGGDATLLVHLGTQAEKDA
                     SVLDQKTDNKEFQIILNLLKDSLKRSPGKWTKIGKGIRGVSEETTTGVKRLYQMLEKG
                     ELLFPAINVNDSVTKSKFDNLYGCRESLADGIKRATDVMLAGKVALVAGYGDVGKGSA
                     QSLRQFGCRVIITEIDPICALQAAMEGYTVTTIEEALPIADIYVTTTGNKDIITIEHI
                     KKMKDQAIVCNIGHFDNEIQVEALENLPGVKKLNIKPQVDKYTLPGGNSIYLLAEGRL
                     VNLGCGTGHPSFVMSNSFTNQTLAQIDLWKNRDSYQVGVYRLPKHLDEEVARLHLERI
                     GVKLTKLTKDQADYIGVDVAGPYKPEHYRY"
     gene            4818..5282
                     /locus_tag="PLF85_05015"
     CDS             4818..5282
                     /locus_tag="PLF85_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNE19053.1"
                     /translation="MSDWRENVELMVQQSKIKVPYTWSVGETGSRFLRALRDEKKLVG
                     NKCPKTGQVFCPPKLNSPYTLEPITEWVELPGTGTVQTFTRRHYSSRAAADGNPNIYA
                     LIKLDGATQLLPHFLGEVDFAQVKHGMRVAAVFRDERVGHILDIKYFKPAEG"
     gene            5323..6480
                     /locus_tag="PLF85_05020"
     CDS             5323..6480
                     /locus_tag="PLF85_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNE19054.1"
                     /translation="MEKSAIIGVAYNKQQRSNTRQNFAEMVYEVTIQALNDAGLDIKD
                     IDNVVTVSNDFWDGRTISSMAIQDACGGYEKNISTVEGDGTFGAYYGLSRILSGSYKN
                     TLVVAHSKGSESDTNLITNGMFEPFFTRPLGLDNITSAAMQARTYMDAFKITEEDLAA
                     VSVKNHGNALKNPKAQVAKQISVADVMASRKLADPLKLYDVSPISDGAAAMIISGDEF
                     TRSLKGGKAKPVYIKGTAFCSDAYDLGLRDLARSPALVAAAKKAYEIAGIKNPGREID
                     LAEIYDAFTHMELMWYEGLGFCHEGKGAEFMKSGATQMNGALPVNASGGVLSAHTVIA
                     AGLIRMIEACLQIRGDAGEHQVGKKVNTALAHGINGPCGQAHCVWVLGKEV"
     gene            6482..7636
                     /locus_tag="PLF85_05025"
     CDS             6482..7636
                     /locus_tag="PLF85_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNE19055.1"
                     /translation="MARRVAVVGSGQTYHTGGRSDVNGQELITEAVTRALKAADLTID
                     EIDGIVIGNMDHFEGINYVDSWSVPANGGVMKPVIKLTTGGTTGTTLAIAGYHMVASG
                     MFSKLLVIGWEKNTESDTTAAITTAFDPIWDRLVFAGAIGGLAVEAQAYLARYNHKKE
                     DAARVVVRDRKHGANNPYAHVQKETTIKQVLGSPLIADPLHWLDMCPRTDGACAVIFA
                     DEHTAEKICDKPAWVLGTSVRHNAAFTTDVSLDGLKSMESASKELFGKTGITDPLNQL
                     DVIEMYLPYSYAGLSWIESLGLCKKGDGPKLVWDGVTDMGGKLPINPSGGPICGNPIG
                     ATALIRVAEATLQIQNRAEVRQVPEAQVALATGFGGCFWTDMMLFGAKKP"
     gene            7633..8112
                     /locus_tag="PLF85_05030"
     CDS             7633..8112
                     /locus_tag="PLF85_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNE19056.1"
                     /translation="MMEENKQGQFILSSGDGAQPYNYAVGTWGSKFLTELRDKEQILG
                     IRCPKCRTVYVPPRQVCGPCFTEMTELVPVASEGKIVSYTILRFQFLDPETGEKKPVP
                     YGYGFFKLDGADNNFQHFIEVPEDESRLYIGARVKAVFAEKKQGSLQDIKHFTLVNE"
     gene            8374..9897
                     /gene="pcnB"
                     /locus_tag="PLF85_05035"
     CDS             8374..9897
                     /gene="pcnB"
                     /locus_tag="PLF85_05035"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNE19057.1"
                     /translation="MSFFQRRKSLSADQILRYPQPKQYNRDQHNVRNSQIDPDAIKVV
                     QRIQQMGCRAYIVGGSVRDLLLGRKPKDFDIVTDAHPQELRRMFANSRIIGRRFRLVH
                     VVFRGGKVIEVSTARSLPSNRAEAKTTDDLYLKRDNQFGTFKEDAARRDFTLNALIYD
                     VKNEMIVDYTGGFEDIQNRVVRVIGNPDISFPEDPVRMYRAVKFAALLNLDLDVNTYR
                     AIQKHKYLLPKASTSRMHEEYNKIFRSGHSAKIFAALNHSGLLETLMPAVARGENLQP
                     ELDEYGFLETGLGRRLSIADRMIQEHEDVNLNIYYALLIAGSLAEVISKNVRDHEKTL
                     ERKLREPLQVLQKDISLTKREFDTMMQIFTVQNNFRRNVTERKGWVKEFQMRHIFQEA
                     FIVYKIIARAAEDEDALQKALFWEIGLRHKLHESIRKNSTENLDIDDNPSEPQQAPQQ
                     PRPARRPQPAATESSPEAGEGPKKRRRRGRRRPRRNSGNAGEGAPAQNNDTPASQDE"
     gene            9869..10585
                     /locus_tag="PLF85_05040"
     CDS             9869..10585
                     /locus_tag="PLF85_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19058.1"
                     /translation="MTLPLLRTNSARSKINLALFGLLAALAAGNVTAQPGSQFIEKSA
                     KRILQRLEKDLREAQVPYPPRAVRLAVFKEERRTELWLPDNQGRWRYVKTWTFSASSG
                     KQGPKIYYGDLQIPEGIYGIDRMEPSKEYHLALHVNYPNDFDRAMLALEGRDPNYVST
                     GINVHGGAISYGCVVIGDRNIEELFLLAHKAGQENTHVYIFPHDTDRTQPQFKSCPEC
                     PVWYGDLLRHLSVAVREFAK"
     gene            10585..>11640
                     /locus_tag="PLF85_05045"
     CDS             10585..>11640
                     /locus_tag="PLF85_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19059.1"
                     /translation="MSAWLIGGADAIQSDIEPQLDDTQSYKKLDLDEKLALFLTTAAK
                     NLAAALNTSFESLRPFITDYISVGASIYERHHTMDVSKKASMLGLSDIELHSIDLGGA
                     SIPAALETAQRLCTQGDRLVLIAGAEVPRGGPTGIAYYREISDALLHKNTELHTHTNL
                     ISLYALMADRMIFEEGVTVEDVEAITRHYRAAAVDDQRAAVCGKTFREGELKRYLAGC
                     YATPMVAVATDHGAAFLVASENMLERLRTAGFELQSPLAIRGVGTNSAAKYLTQRRDF
                     RSPAALAAERAFARARLTPGDVDYAWIYDCFTLMLVRQAADYFGQNAQAAAQTLRSGY
                     LQNGTKKILVNRRGGILN"
BASE COUNT         2707 a         3267 c         3146 g         2520 t
ORIGIN      
        1 cggcatcatg accttcatct cgctggccac cttcatcggg cttttggccg tactcgggcg
       61 ctggcaggtt tcgggcgaag gcatgcgcct cagcctgcgc tttcggttcc tgaataatct
      121 ctctaccggg gccccggtcc gtatatccgg gggattgcct gtcggttacg tagaaaatat
      181 ctacgaaaaa gacctgcgca cctacgtgca gatttcactg cgtaaagacc tgcgcgacaa
      241 gatacctaaa cgcgccgaga cggtttttgc catctacacg acgggcctga tgggacagaa
      301 atatatcaat atcactgttc ctgatccaaa gccagaagac accttcttta aagacggcga
      361 cgagtgggtc ggcattgatc cgccttctgt cgaccagatg atgatggcat tctcgagctg
      421 gttcgacggc aagaacggcg gccaggtact cgccgaaatc atgaaagaga ccgaaacctt
      481 tctcgctaac ctcaacggca tcgtgaacga aaaccgtggc gatatcaggc agaccatacg
      541 ccaggcgcgc gaatcgttta cttcgctctc tgcgcagctc gatacgctca tgcagaagct
      601 gaatatcctc tccaagaact tcacagacat ctcgaaccag aacaagcagg atatacagat
      661 catgctagag aacatgtcga agatctcccg tgacctgaac ctgatcacgc agcgcgtgaa
      721 ttctggccgt ggttcggtcg gtaagctgat ccaggacgaa gagctctaca agaacgcaaa
      781 cgaagcggcc taccacgcgc gcgagctttt caagaagctc gaagaaaatc cctcgctctt
      841 tctcaacaag agcaactgat atggcgctgc cgctcgacgc cacgcgacgc gcatcggtcc
      901 gggccggcag tttcgtgctg ctgggcttcc ttgtcctcat cctctcggca attattggcg
      961 cacggataca tacagcgcgc gcaggcttca cgatcgacat tacattctcg ttcctgagca
     1021 acctcacccg caatgcgaaa gtgctgcttt cgggcggccg ccaggtcggc tacgtgcagg
     1081 atatcttcca aaaagatcaa cagacgtatg ttcgccttta tctcgagaac tcgctgcggg
     1141 gcaaaatgcc caacacctct gacacgcaga tttcgatctt ctccaataac ctgatgggac
     1201 agaagtatat caacatccag ttccatgacc tcaaacccaa ccaacctgtc atcgaacccg
     1261 ggcaggttgt gcgcggcatt tcgcccccgt ctttcgagca gatgatgctg tcgttttcca
     1321 gttggttcga ggggcgcagc gcaggcgagg tggcggagct catttttgcc aaggcagcga
     1381 tgctgaggtc aaatatcgac gcggtaatga acgaaaaccg tgaagatctc gcggcaacca
     1441 tgtcgggtgc caaaaactat tttaccgcgg tctcggacca gtttgaaagc ctcaaatcga
     1501 acatctctct gattgcgcga aacagcgaag agatactgac tgcgcagcag caaagcctga
     1561 cacagatggt atccaatagc gccgcaatgg cacaaaacct cgaagcgctc gagaaggcac
     1621 tctcgaataa tcgcggcagc ctgggtaaat tcaataaaga aagcaaggta ttgcgcgaaa
     1681 acgtccggca gaccattgaa tacagccggt ctttcattaa atgtatacaa gaacggccgt
     1741 gggtgattat ctacaaagaa tcctgccgcg aacgctgagt ttacggcttc tttacaggag
     1801 cctgttagtt ttcttctgcc gagatacggc gcaagagttc ctcgcggtgt ttttcatcga
     1861 gcagctgcgc ggtttcagta ccgggctttt tgcgacgtcc cctgatgaaa agcatgacta
     1921 cacccgcgca gagtatgaac ccaaacagcg aaaatattcc gaggtagttg ctttcgggta
     1981 tactcagaat ctggctacca aaaccatcgc gaaactgcgc tgccatataa ccataatcgg
     2041 gagtgcgcgc catcgcgaaa aattctgctg actccgcatt cttgccaaaa ccgttacgaa
     2101 agccgttcag gatctggtca tcagtataac cggccatgag caatttgtcg agagcatcac
     2161 gctgcggcca ggcattgcag tgacccgtgt ggttgcagtt tctcagcggc atgttgcagc
     2221 cacaggtgca catcagtttg cgcgaaagac tgtcgaagcg cttcaaaagc gtcgtgtccg
     2281 tgagccggct gtgcgcactc tgtgcgacca attgcccgct atttgctgtt gccaggctca
     2341 gtaaaatcag gccggtggat aagaagcgct tcacaactat caatataatg aaattcacct
     2401 gccgcataaa gattattttt gttgtgctgc tcatgagctc tgtgccactg tgggcatcag
     2461 attgggagac aggtctcacc ggcgcctggt cggtcgcttc acggcaattg acggccagca
     2521 ccgcctatac agccggcagc gaggactcgc gtttcatcga ctttggtggc cgggtggcgc
     2581 acaggcttgg gccgggattt ctgggcgtgc attttggcct ctattcatcc ttcggtctgc
     2641 gcgaacaggt taccgcatcg aatgcacatg cgaccgattt tgtggtaaac aagtcagcgg
     2701 aaacaggcct accggtctcc ttcgattcag gtacctaccg gctcgcgctc ggctggaatg
     2761 cactgcagct tcgctaccgc tactatctca ctgagtatct ctttgctgaa gccggcgctg
     2821 gtgccgccta cggttttggc ggtatcgaag cagcctttaa aggaagtaat tctgcaggaa
     2881 gcgtgagctc cacctactac caccgctatt ctgaatgggg ttttatcagc tctgccagcg
     2941 caggcatcag cctgccgctc ggtgacacaa tccggctgga agcggcatgc gagttcgcct
     3001 ggttatgggc tcagatccgc gagccgaaca tttttcgcac tggggatttt accctgtcac
     3061 agaccttttt ccgcccgatg gtgggcatcg ttctgaaatt ctgaggcggg attattgacc
     3121 tgcaccgcga aacggcagac cgcttcacgg gcgcaggtac cgttattaat tttccttcgg
     3181 aaaattaata acggtaatgt tccggcttat aaggacccgc tacatcaacg ccgatgtagt
     3241 ctgcctggtc ctttgtgagc ttggtcagtt ttacaccgat ccgctcgagg tgcaggcgcg
     3301 ctacttcttc gtcgaggtgc ttgggcaggc ggtagacgcc aacctggtag ctgtcgcggt
     3361 ttttccagag gtcgatctgc gcgagtgtct ggttggtaaa cgaattactc atcacgaatg
     3421 acgggtggcc ggtaccgcaa ccgaggttca cgaggcggcc ttcggcgagc aggtagatcg
     3481 aattgccacc gggcagagtg tacttgtcta cctgcggttt aatattgagc ttctttacac
     3541 ctggcaggtt ctcgagcgct tccacctgga tttcgttgtc gaagtggccg atgttgcaga
     3601 caatcgcctg gtctttcatc ttcttgatgt gctcgatagt gatgatatct ttgttaccag
     3661 ttgtggtcac gtagatgtca gcgatcggga gtgcttcttc gatcgttgtc acagtgtaac
     3721 cttccatcgc cgcctgcagt gcacagatag ggtcgatctc ggtaatgatc acgcggcaac
     3781 cgaactggcg cagtgactgc gcagaacctt tacccacgtc gccgtaaccg gcaacgagcg
     3841 caactttacc ggccagcatg acatccgtcg cacgcttgat accgtcggcg agtgattcgc
     3901 ggcaaccgta caggttgtcg aactttgact tggtgaccga atcgttcaca ttgatcgccg
     3961 ggaagagcag ttcgcccttt tcgagcatct ggtacaggcg cttcacgccc gtagtggtct
     4021 cttcagacac accgcggata cctttgccga tcttggtcca cttgcccggg ctgcgcttga
     4081 gtgaatcttt cagcaggttg agaataatct ggaattcttt gttgtcggtc ttctggtcga
     4141 gtacagaagc atctttctct gcctgcgtgc cgaggtgaac gagcagtgtc gcatcgccgc
     4201 cgtcgtcaac gatcaggtcg gggcctgaac catcgggcca ggtgagtgct tcttcagtgc
     4261 accaccagta ttcttcgagg gtttcgcctt tccaggcaaa caccggtgtg ccgctcttgg
     4321 caattgctgc cgctgcgtgg tcctgcgtag agaagatgtt gcagctgcac caacgaacat
     4381 ctgcaccgag ttctttgagg gtttcaatca atacgccggt ctggatggtc atgtgtagtg
     4441 aacccatgac ttttgcgcct ttcaggggtt tctctgcgcc gtatttcttg cgcagcgcca
     4501 tcaggcctgg catttcgtgt tcggcgatgt cgagttcctt gcggccaaat tccgcgaggt
     4561 tgatgtctgc taccttgtaa ggtagttgtc cgatggcaac gccagcggga gtttcagcag
     4621 ttttcatatg gatcgataca tctaaatata tcgatgcgat gtcaacctct atccacctgc
     4681 gcgtggtaga tgctggacaa gcgggcgtga caaaaactgg ctgataatcg agttcagagt
     4741 cgcttttgta gcgtttgcca caggaaattt ggatcttttt gtttggcaat ctgtcactgc
     4801 tttctgagcc tacccgcatg agcgattggc gcgagaatgt tgaattgatg gtccagcaga
     4861 gcaagatcaa ggtaccgtat acgtggtctg tcggtgaaac cggctcgcgc tttttgcgcg
     4921 cgctgcgcga cgagaagaaa ctcgtgggga acaagtgccc gaaaaccggc caggttttct
     4981 gcccgcccaa gctgaactcc ccctacaccc tcgagccaat caccgaatgg gttgaattgc
     5041 ccgggacagg caccgtgcag acattcacgc gccgccacta cagctcacgt gcggctgcag
     5101 acggcaaccc caacatctac gctctcatca agctcgacgg cgcgacccag ctgctgcctc
     5161 attttctcgg tgaagtggat ttcgcccagg tcaaacacgg catgcgtgtt gcggcggtct
     5221 ttcgtgatga gcgcgtaggg catattctcg acatcaaata tttcaaaccg gcggagggct
     5281 agtagagacg cataattttg cgtctacaca taggtcatag ttatggaaaa atctgcaatc
     5341 atcggcgttg cctacaacaa gcagcagcgc agcaacacgc gacaaaactt cgccgaaatg
     5401 gtctacgaag tcacgataca ggcgctgaac gacgccggcc tcgatattaa agatatagat
     5461 aacgtcgtca ctgtttcaaa cgacttctgg gatggccgca cgatttcatc gatggcaata
     5521 caggatgcct gcggagggta tgagaagaat atctcgaccg tagaaggcga tggcacgttc
     5581 ggcgcgtatt acggtctctc gcgtatactt tccggctcgt acaagaacac gctggttgtg
     5641 gcacacagca agggttcgga aagcgacacc aacctgatta ccaatggcat gttcgagccc
     5701 ttctttacgc gaccgctggg actcgacaac attacgtctg ccgccatgca ggcgcgcacc
     5761 tacatggatg cattcaaaat aaccgaagag gacctcgctg ctgtgtcggt gaaaaaccac
     5821 ggcaatgcgc tgaagaaccc gaaagcgcag gtggcaaaac agatcagcgt agcggatgtt
     5881 atggcctcac gcaagctcgc cgatccgctg aagctctatg acgtttcgcc gatctctgat
     5941 ggtgcggcgg ccatgattat ttcgggcgat gagttcacgc gcagcctcaa gggcggcaaa
     6001 gctaaacccg tatacattaa agggactgca ttctgttctg acgcctacga cctgggcctg
     6061 cgcgatcttg cgcgctcgcc ggctctcgtt gcagcagcaa agaaggctta cgaaatcgcc
     6121 ggtatcaaaa acccaggcag ggaaattgac ctggcagaga tctacgacgc cttcacccac
     6181 atggagctca tgtggtatga aggtctcggt ttttgccacg agggtaaggg tgccgagttt
     6241 atgaaaagtg gtgcaacaca gatgaacggg gcattgcccg tcaatgcctc gggcggagtt
     6301 ctctctgcgc acacggtcat cgccgcgggg ttaatccgca tgatcgaagc ctgcctgcag
     6361 attcgcggcg atgccggtga acaccaggtc ggaaaaaaag tcaatacggc gctggcgcac
     6421 ggaatcaacg ggccatgcgg ccaggcgcat tgcgtctggg tactggggaa agaggtttaa
     6481 catggcacgc agagtagcag tcgttggctc aggccagact taccacaccg gcgggcgcag
     6541 cgacgtcaac ggccaggagc tgatcaccga ggcagtcacg cgtgcgctca aggcagcgga
     6601 tctcacgatc gatgagatcg atggtatcgt gatcggcaac atggaccact ttgagggtat
     6661 caactatgtc gattcatggt cggtaccggc aaacggcggg gttatgaagc ccgtgatcaa
     6721 gctgaccacc ggcgggacaa ccggcacgac gctggcaatc gccggctacc acatggtggc
     6781 ttccggcatg ttttcaaaac tgctcgtgat aggctgggag aaaaacaccg aatcagacac
     6841 gactgcggcg atcaccaccg cgttcgaccc gatctgggat cgacttgttt tcgcgggcgc
     6901 gattggtgga ctcgccgtcg aagcccaagc ctatcttgcc cgttataacc acaagaaaga
     6961 agatgcagcg cgcgttgtgg tgcgcgaccg caaacacggc gccaacaacc cgtacgcgca
     7021 cgtgcaaaaa gaaacaacga tcaaacaggt tctgggctct ccgctgatcg ccgaccccct
     7081 gcactggctc gacatgtgcc cgcgtaccga cggcgcgtgc gctgtcatct tcgccgatga
     7141 acacactgca gaaaaaatct gcgacaaacc ggcgtgggtg ctgggtactt cggtgcgcca
     7201 caatgcggct tttaccactg acgttagcct cgacggtctc aaatcgatgg agagtgcctc
     7261 gaaggagctc tttggcaaaa ccggaatcac cgacccgctg aaccaactag acgtcatcga
     7321 gatgtacctg ccctattcgt acgcaggcct aagctggata gaatcgctcg ggctctgcaa
     7381 aaaaggcgac ggccccaaac tggtctggga cggcgtgact gacatgggcg gcaaactacc
     7441 gatcaatccc tcgggtggcc cgatctgcgg taacccgatt ggtgcgacgg cgctgatacg
     7501 tgttgccgaa gccacgctgc agatacagaa ccgcgcggag gtgcgccagg tgcccgaagc
     7561 acaggttgca ctggcaaccg gtttcggcgg ttgcttctgg accgatatga tgctttttgg
     7621 agcgaaaaaa ccatgatgga agagaacaaa caaggccaat tcatcctgtc gtcaggcgac
     7681 ggcgcgcagc cctataacta tgccgtcggc acgtggggta gcaagttcct gaccgaactg
     7741 cgcgacaagg aacagatact cggtattcgc tgccctaaat gccgcacggt ctacgtaccg
     7801 ccccgacagg tctgtggtcc ctgtttcact gaaatgaccg agctggtgcc tgttgcctcc
     7861 gagggcaaga tcgtcagcta taccatcctc cgcttccagt ttctcgaccc cgaaacgggc
     7921 gagaagaagc ccgtgcccta cggttacggt ttcttcaagc tcgacggcgc cgataacaat
     7981 ttccagcact tcatcgaggt gcccgaagac gagagccggc tctatatcgg cgcccgggtg
     8041 aaggctgtat ttgcagagaa gaaacagggt tcgctgcagg acataaaaca ttttactctg
     8101 gtgaacgaat aaccggtttt cggccctccg cccggcagct gcgacggagg gttttgcaga
     8161 attaggttga gagcctgtca ccctattgag aatctgcccg agtgccggcc aaaaagtccc
     8221 gactgctaac cccaggtcga gatcaggcat acgctcttcg ctctcttgaa ttgcccctga
     8281 ttttggcttt ttaccccgtg cccaatacag cttcctcaca agctgaggaa tactcgatag
     8341 agaaggacac tgctttccat gttacagaag atcatgagtt tttttcagcg acgcaagtcg
     8401 ctcagcgcag accagattct gcgttatccg cagcctaaac agtataaccg cgaccagcac
     8461 aacgttcgca acagccagat cgaccccgac gccatcaaag ttgttcagcg tatacagcaa
     8521 atgggctgtc gcgcgtatat cgtcggcggt tctgtccgtg atctgctgct aggccgcaag
     8581 ccaaaagatt ttgacatcgt taccgacgcc cacccgcagg aactccgccg gatgtttgcc
     8641 aatagccgca tcatcggccg gcgtttccgc ctggtgcatg tggtctttcg cggtggcaag
     8701 gtaatcgaag tttcgaccgc ccgttcgctt ccttcaaacc gggcagaagc aaaaaccacc
     8761 gacgatcttt acctgaaacg ggacaaccag ttcggcactt tcaaagaaga cgccgcgcgc
     8821 cgcgacttca cgctgaatgc gctcatctac gatgtcaaaa acgaaatgat cgtcgattac
     8881 acgggtggct tcgaagatat tcagaaccgc gtcgtacggg tcattggcaa tccagacatc
     8941 tcgtttcccg aagacccggt gcgcatgtat cgcgcggtga agtttgctgc actgctcaat
     9001 ctcgatctcg acgtgaacac ttaccgcgca atccagaagc acaagtacct gctacccaaa
     9061 gcttctacct cgcgcatgca cgaagagtac aacaaaatct tccgcagcgg tcactcggcg
     9121 aaaattttcg ccgcgctgaa ccattccggc ctgctcgaga cgctaatgcc cgcggttgca
     9181 cgcggcgaga acctgcagcc tgaacttgac gagtatggtt tcctcgaaac agggctgggc
     9241 cgtcgcctga gtatcgccga ccgcatgatc caggaacatg aagacgttaa cctgaatatc
     9301 tactacgcgc tgctcatcgc gggttcgctt gcagaagtca tcagcaagaa cgtccgtgat
     9361 catgaaaaga cgctggagcg caaattgcgc gaaccactac aggtactgca aaaagatatc
     9421 tcgctgacca aacgcgagtt cgacacgatg atgcagatat tcacggtgca gaacaatttt
     9481 cgccgcaatg tcaccgagcg caagggctgg gtaaaagaat tccagatgcg ccacattttc
     9541 caggaagcct ttatcgttta caaaattatt gcccgcgccg ccgaagacga agacgctctg
     9601 caaaaggccc tcttctggga aattggtctt aggcataaat tgcacgagtc gatccgcaag
     9661 aattcgacag aaaatctgga tatcgatgat aaccccagcg agccacagca ggctccacaa
     9721 cagccacgcc ctgcccggcg accgcaacct gcagcaactg aatcttcacc tgaggcaggc
     9781 gaaggaccca agaagcgtcg tcggcgtggt cgccgccggc cacgcagaaa ttctggcaat
     9841 gcgggtgaag gtgcgccggc gcaaaacaat gacactcccg cttctcagga cgaatagcgc
     9901 ccgttcgaaa attaacctgg cattgtttgg cctgctcgca gcgttagcag cgggcaatgt
     9961 gactgcgcaa ccaggcagcc agtttattga gaagagcgcc aaacgcattc tgcaaagact
    10021 tgaaaaggat ctgcgcgagg cgcaggtccc ctacccgccg cgcgccgttc ggcttgcggt
    10081 attcaaagaa gaacgccgca cagaactttg gttgcctgac aaccaggggc gatggcgcta
    10141 cgtaaagacc tggacttttt ccgcgtcgag tggtaagcag ggccccaaga tctattacgg
    10201 cgacctgcag attcctgaag gcatctatgg catcgaccgt atggagccca gcaaagagta
    10261 ccatcttgcg cttcacgtaa attatccgaa tgatttcgac agggccatgc tggcgctcga
    10321 agggcgtgac cccaactatg tttctaccgg gatcaacgtg catggcgggg caatcagcta
    10381 tggctgcgtt gtgatcggcg atcgtaacat tgaagaacta ttccttctcg cacacaaagc
    10441 cggacaggag aatacccacg tgtatatttt cccccacgac accgaccgca cgcaaccgca
    10501 gttcaagagc tgtcccgagt gccctgtctg gtacggcgat ctgctgcgcc atctttctgt
    10561 tgccgttagg gagtttgcaa aatgatgagc gcatggctca tcggtggtgc cgatgccatc
    10621 cagagtgaca tcgaacccca gctggacgac acgcaaagct acaagaaact ggacctggac
    10681 gagaagctcg ctctctttct tacaacggcg gcaaagaatc ttgctgctgc actcaacacc
    10741 agtttcgaat ccctgcggcc attcatcacc gactatatca gcgtcggtgc atctatctac
    10801 gagcgccacc atacgatgga tgtttccaag aaagcttcaa tgcttggact ctcagatatc
    10861 gaactgcaca gtattgatct tggtggcgcc agcattcctg ctgcactgga aaccgcgcag
    10921 cgactctgta ctcagggcga caggctggta ttgattgccg gcgccgaggt gccgcgtggc
    10981 ggccctaccg gaatagctta ctatcgcgaa atcagcgatg cgctgctaca caagaatacc
    11041 gaactccaca cgcataccaa cctgatatca ctctatgccc tgatggctga ccgcatgatt
    11101 tttgaagagg gcgtgacggt cgaagacgtc gaggccatta cgcgccatta ccgtgcagct
    11161 gcagtcgacg accagagggc tgcagtctgc ggcaagacat tccgtgaggg ggagctcaaa
    11221 cggtacctcg ccggctgcta cgcgacgccg atggtcgccg tggctacaga ccacggggcg
    11281 gcattcctgg ttgccagtga aaacatgctc gaacgattgc gcaccgcagg attcgagtta
    11341 cagtcgccgc tggcaattcg gggcgtgggc accaacagcg cagccaagta cctgacgcag
    11401 cgccgcgatt ttcgctcacc tgcagctctt gctgcagaac gtgcctttgc ccgcgcgcgc
    11461 cttacgccag gtgatgtcga ctacgcgtgg atctacgact gcttcaccct catgctggtc
    11521 cgccaggcag cggattattt cgggcagaac gcccaggcgg cagcgcaaac cctgcgcagt
    11581 ggatacctgc aaaacggcac aaagaaaatc ctggttaacc ggcgcggtgg aattctcaat
//
LOCUS       DAOQJF010000063        11624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2792_length_11624_cov_6.265710, whole genome shotgun sequence.
ACCESSION   DAOQJF010000063 DAOQJF010000000
VERSION     DAOQJF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_11624_cov_6.265710"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /gene="gyrB"
                     /locus_tag="PLF85_05050"
     CDS             complement(<1..1896)
                     /gene="gyrB"
                     /locus_tag="PLF85_05050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNE19060.1"
                     /translation="MHGHVSEEYNASKITVLEGLEAVRKRPGMYIGSTDLPGLHHMVY
                     EVVDNCIDEAMAGHCDTIEVEISKGNVISVSDNGRGIPVDIHPKVGVSTVEVVLTKLH
                     AGGKFGDGAYKVSGGLHGVGVSVVNALSEWVEVDVHLNGKQHHMHFDRGKPRAPLKVK
                     GPSKKRGTTVTFLADNTIFDTLEYRYDTLSARLRELAFLNKGVKISIRDTREKEVKEH
                     VFQYKGGIVEFVKMLTEKKNPLQKKPIYAIQQKDGIQVELAIEYCDTYSEQIFTFANA
                     IHTREGGTHLEGFRTALTRAINEYKKKLGYDKKVEEPLTGDDVREGLTLVLSILIPNP
                     QFEGQTKMKLGNGEVKGIVTSVVYEKLCEYFDENPADAKRVIEKCMHAALARIAARKA
                     KELVRRKSALEGAGLPGKLADCSARDPKDCELFIVEGDSAGGSAKQGRNRHFQAILPL
                     KGKITNVEKTKLDKILGDGEVAALISAIGCGISAEFNIEKLRYHKIVIMTDADVDGAH
                     IQTLLLTFFFRHMGALIEQGCLYIARPPLYLITQGKEKHYAYTDQEKDKILKGFKSES
                     KYIIQRYKGLGEMNPEQLWETTMDPARRVLMQVSVDDAVIADETFVILMGDEVDPRRK
                     FIEDNAKK"
     gene            2086..3288
                     /locus_tag="PLF85_05055"
     CDS             2086..3288
                     /locus_tag="PLF85_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE19061.1"
                     /translation="MPEIRIKDYKAGTAVIIQNAPNPGLFYIVRKGVLAIDTEHRLND
                     KVLSRFEAGDSFGLVSALTGHHFLVTIYAATDAQVAEIPISMIGSYLKSQSELAIKML
                     RLYSRELRTIQLHLAKLDTPENRVITPDVLYNLARKYIDWQKPQYAAQALKAYLEWAE
                     TNEGVYLQMAKDLWPQVQSSYQELQFTGKGTVVPADTMIFSEGEMSRDIYVVLAGSVK
                     MMRIVHGDEFIIDVLGPGELFGEMAFIENAPRMGSAITTQESTLIRIKPEQLVSSVGE
                     GVLQKIFENMARRIWFSHQRLIIFKIRDPLVRVYAYLYNLVRHQNLRTKNRSAEDKSY
                     RFDITMTELKTLCGILRLTESTERALHENHNLIFDDTSITIKSRKRLEDQIAYYRAKS
                     GQIIAETK"
     gene            complement(3324..5402)
                     /locus_tag="PLF85_05060"
     CDS             complement(3324..5402)
                     /locus_tag="PLF85_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19062.1"
                     /translation="MRLLALLLLPMLSLGAAGKELTFYLTANTGGRFPLNNELQENEL
                     MRLAAYLRSAKQKNPAAYHLDLGNAFFPGRLSRFSFGSLTADYLQMLRLDAGLVAAGD
                     LNIGAESLDYIRRARGIRLLSANIIRDKSPFFEPYAILQKGGVKTALLGITSNKSLVG
                     YAEAQLLALHVDPPEATLTAPLAQIALEKPDLTIAMSGVPLRDGIVLLSKNPQIDILL
                     CGGDAEGSLGKEAVRQVELPDGRRVIAMPKENPLMRIVLRQEGKRWVLAEREIVDLES
                     AEDSVSVPPTFERRLGLWQKWYATSEGDGAGKVSFTPFRLTPQFAAASLRASFGCDVA
                     FVEDDDLDADEAAEVKNQQDIRYAVQNDYDIFTFRLTGAELRDFYRKNPDLVFSGMNA
                     QSVTGYPIRDNVSYRICATQRGYEYAMRSDRRTAGRNQWLGISDSVSRTLEEKITEPD
                     KYADSRFRFLALLNLSNVYETGRVVNTSNIDTPPGQAADSYFKWGLENDLNFMLYNRR
                     HSFAFNPYIFYVRQMDQIIRNLLRADITYTFNTEWYLRPYQKNRLDTVVVPVNGQRPS
                     FLRETVGAEFNYKVFTGRLGGGLEKEILDPVNDPNWGFEASIGVLWPFYTNMQYKLAF
                     DSFSSRTYQDFWRHRIEIANSVIFTVAAPITFTVSHRWYYFYLGSVGDFYNSSILLLS
                     LDVRTTWKEP"
     gene            complement(5399..6055)
                     /locus_tag="PLF85_05065"
     CDS             complement(5399..6055)
                     /locus_tag="PLF85_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19063.1"
                     /translation="MRSLLVLPILAATCVAASGCSVFARRERTPDQIAAEAALMGKKT
                     ALLERENQVLREENLALNRDAELQKSEQQKKQAEFSAERDKLTAQVKSAEGAIANLNE
                     KIGILESESGGKIRQLVALNEQLAKKSAEEMRKAQEDLSRVQLESAQEKEKLGRENAE
                     KQFALGKEIQDLRQRLAEKEKETDELRRKVAELRAAEAQLTQQLGELRQKLGSTPEKP
                     "
     gene            6205..6861
                     /locus_tag="PLF85_05070"
     CDS             6205..6861
                     /locus_tag="PLF85_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE19064.1"
                     /translation="MENSGKKSLKEAMANSETIRMFKWDWMERLSHVHPATPFVIYIP
                     VVVIAMYYAVLQGNLTIAQMVAAFVGGMLVWTLMEYLIHRFLFHIPQTNRVFKAIYFY
                     SHGIHHDAPNDATRLVMPPSASIPLAILFYYLFEGVAGIYNLPLFAGFLTAYMWYDFI
                     HFSTHFFNWKWAWFRKIKENHMRHHFLTNKYNFGLSSPLWDYVFFTLFKGKASLGDKQ
                     "
     gene            7036..8205
                     /locus_tag="PLF85_05075"
     CDS             7036..8205
                     /locus_tag="PLF85_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19065.1"
                     /translation="MKRTIKIGFVMLVASASWLIAQDLNQPALDSADKAAQDAEKKVD
                     EAEKAAEGKVEVPATSERAAEKPAGTQPATADNQVGNIYNDGKTTYATSQAKFELTAT
                     DNISNLDFIEYKINESYQFSVYGHPFSLEKEGPHKITYRSVDRVGNREEDNIFNVIID
                     NTGPYMNFALNKAPIVIDGKRYLAPGTKLEIRATDNYSGVKSVEYSTNGSDWQPYKDG
                     IVLDKPGAAQLKYRAIDNLGNKSENLSGFSTTTPGQGASPEDLAVINSLYVENTPPVV
                     MITPAKRWINVDGKKYAMRDNTYLIEAQDAESGVANIFVKVDNGAEWQIYSGKLAFQT
                     EGPHSIEAKAVDKVGNESQPVKVEFIVDDNPPKSTIRPVGTTAEKAAEPAPAPAQ"
     gene            8216..8854
                     /locus_tag="PLF85_05080"
     CDS             8216..8854
                     /locus_tag="PLF85_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HNE19066.1"
                     /translation="MLTRSPAIAGLFFSLVISAAEAPQLSASGQAYFRRFVEQFTPLV
                     VRNEKRDRRLNFRWDTTNRDCAGLVRYVFHEALSQHRESFFTHYPQMARLGNYAGGAE
                     FSLAGRYWSEGNQTAPDILRHARYVGRETAEPKLKTGDILYYESAEWRIRHVMLVVRS
                     GKSVFLVYHTGDKRDELRLRTVDDMRRQDEVQWHPAPDNPVFRGVYRPNFLQ"
     gene            8922..9479
                     /locus_tag="PLF85_05085"
     CDS             8922..9479
                     /locus_tag="PLF85_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19067.1"
                     /translation="MFGIRAAWLAVLIVALAACGKKKLETPEAALSAAQEAYLKRDAK
                     AFIAVLSKETLSELEEKIESMRTMFQSMPENPQAAKAFEHMAKQMNMPVSKLKELTIE
                     DFIGYMMQNEAASGSETTLLPKEMLPPAKVVRRTEKGNKATLYFEDDEKLSFIKTDAG
                     WRVHLDAENVAAKGMPMQGNEEREE"
     gene            complement(9522..10061)
                     /gene="hslV"
                     /locus_tag="PLF85_05090"
     CDS             complement(9522..10061)
                     /gene="hslV"
                     /locus_tag="PLF85_05090"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNE19068.1"
                     /translation="MQIHATTILCVKRGNQVAMAGDGQVSAGQTILKGNAVKVRKLLN
                     GKILTGFAGATADAFTLLEKLEEKLQAFGGNLTRAAVELAKDWRTDKMLRRLEAMLIA
                     ADQEKIFLLTGNGDVIEPEEGVVAIGSGGNYALAAARALVKNTHLSAKEVCLESLRIA
                     AEICVYTNGSITLESLEGP"
     gene            complement(10083..11057)
                     /locus_tag="PLF85_05095"
     CDS             complement(10083..11057)
                     /locus_tag="PLF85_05095"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNE19069.1"
                     /translation="MSDFKPQRARLPVPGEPGIFGLYEGPIADTKTHEWDFNSKLFPR
                     RRTERKAWIYLGVFSRDLYAGMAIVDAGYLSSAFSYVYIPSEKFFIEDRQTRPMGFAT
                     DFDATLNDTWRLGPYEILPTQNKIVFNMRGQFDLSATFTLRHNGLSTIAPSDSMRPFH
                     FTYKEMAMAVDGEFIGQGRRQKFSGDFGILDYSKGYPPRRTEWNWLSMVGKTESGKSL
                     AVNLVDKFNEGIENALWLDGKPMALSTAHFHYIRPAEKSIWRILTRDNVFEARFRPFG
                     ARSENLNAGLLRSQFVQPYGTFDGQVKIGEETEKFTGFGVTEDHLALW"
     gene            complement(11061..>11624)
                     /locus_tag="PLF85_05100"
     CDS             complement(11061..>11624)
                     /locus_tag="PLF85_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE19070.1"
                     /translation="DTGSDCVGTSNRHGAASVTQLELFPMPPLERAASTPWPYWPMKL
                     RTSSSHEEGAERMWSINTVRFNDDGKGNVKSLSCKKVEMKDGKFVDVDGSEFELKAEL
                     VLLAMGFVGPVKGGLLEQFEKLGLTLDNRGNVKADFGDHSGAHRTTIDKVFAAGDIRR
                     GQSLIVWAIAEGRKCANAVHSYLMQQK"
BASE COUNT         2718 a         3184 c         3046 g         2676 t
ORIGIN      
        1 tttcttcgcg ttgtcttcga tgaacttgcg gcgggggtcg acctcgtcgc ccatcagtat
       61 cacgaatgtc tcgtcggcga tcactgcgtc gtctaccgac acctgcatca gaacgcggcg
      121 cgccgggtcc atggtggttt cccagagctg ctctgggttc atctctccga gacctttata
      181 gcgctggata atgtatttcg attcgctctt gaaacccttg agaatcttgt ccttttcctg
      241 gtcggtatag gcgtagtgct tttctttacc ctgcgtgatc aggtagagtg gcgggcgggc
      301 gatgtaaagg caaccctgct cgatgagcgc gcccatgtga cggaagaaga aggtcaaaag
      361 cagcgtctgg atatgcgcgc catccacgtc ggcatcggtc atgatgacga tcttgtggta
      421 gcggagtttt tcgatattga actcggcact gataccgcag ccaatcgccg agatcagtgc
      481 tgcaacttcg ccgtcgccca ggatcttatc gagcttggtc ttttcgacgt tggtgatctt
      541 acctttaagc ggcagaatcg cctggaagtg ccggttacgg ccctgcttcg ctgagccacc
      601 cgccgagtca ccctcgacaa tgaagagctc gcagtctttg gggtcacgcg cactgcagtc
      661 ggccagttta ccgggcaggc cggcgccttc gagcgcgctc ttgcgtcgca cgagttcttt
      721 ggctttgcgc gcggcaatac gagccagcgc agcatgcata catttttcaa tcacgcgctt
      781 ggcatcagca gggttctcgt caaaatactc gcagagtttt tcatagacga cagaagtgac
      841 aatccccttt acttcgccgt tacccagctt catcttggtc tggccttcaa actgcgggtt
      901 aggaatcagg atagaaagca cgagcgtgag accctcacgc acatcgtcgc cggtcagcgg
      961 ctcttcgacc ttcttgtcgt aaccgagctt cttcttgtat tcattaatcg cacgcgtcag
     1021 cgccgtgcgg aaaccttcga ggtgcgtgcc accttcgcgc gtatggatcg cgttggcgaa
     1081 ggtaaagatc tgctcagagt aggtgtcgca gtattcaatc gcgagctcaa cctggatgcc
     1141 atctttctgc tgtatcgcgt agattggctt cttctgcagc ggattcttct tctcggtcag
     1201 catcttgacg aattcaacaa tgcctccctt gtactggaag acgtgctctt tgacttcttt
     1261 ttcgcgtgtg tcacgaattg aaatcttcac acccttattg aggaaggcaa gttcgcgcag
     1321 gcgcgctgag agcgtatcgt aacggtactc gagcgtgtcg aagatagtat tgtcagccag
     1381 aaacgtaact gttgtaccgc gcttcttgct tggccccttg accttgagcg gagcacgcgg
     1441 tttgccacga tcaaaatgca tgtggtgctg ctttccgttg agatgtacat cgacttctac
     1501 ccactcagaa agtgcgttca caacggaaac acccacaccg tgcagaccac cggaaacctt
     1561 ataagcgccg tcgccgaact taccgccggc atgcaactta gtgagcacga cctcgacagt
     1621 agagacaccc accttcgggt gaatatcgac cgggatacca cggccgttgt ctgagacgct
     1681 gatgacatta cccttgctga tttcgacttc aatagtatcg cagtggccgg ccatcgcttc
     1741 gtcgatgcag ttgtcgacga cttcgtagac catgtggtgc agacccggca ggtctgttga
     1801 accgatgtac atacccgggc gcttacgcac cgcctcgagg ccttcgagaa cggtaatttt
     1861 cgaggcgttg tactcttcag agacgtggcc gtgcatcggc tggttttctg cctcttcggc
     1921 ctttgccttg gtggccggct ttttttcgtc ttttttcaca ggttttgcca tggtttgtcg
     1981 gccattttag caaaatcaga tattatgtcg ccttctttaa taaaggtacg gagcagacca
     2041 ataggctcta ctctgtcact ccggcggccg ataattacct aaggcatgcc cgaaatccgc
     2101 atcaaagatt acaaggccgg taccgcggtt atcatccaga acgcgcccaa ccccggcctt
     2161 ttctatattg tccgcaaggg tgtactcgcg atcgacaccg agcaccggct gaacgacaaa
     2221 gtgctttcgc gctttgaggc aggggacagc tttggcctcg tctcggcgct gaccggccac
     2281 cattttcttg tcaccatcta tgccgcgact gacgcgcagg ttgcagagat acccatctcg
     2341 atgatcggta gctatctcaa aagccagagc gagctcgcga tcaagatgct caggctctat
     2401 tcgcgtgaac tgcgcaccat acagttgcac ttagccaaac tcgatacgcc agagaaccgt
     2461 gttattactc ccgatgtact ctacaacctg gcgcgcaagt acatcgactg gcagaaacca
     2521 cagtacgctg ctcaggcgct gaaagcctac ctcgaatggg cagaaaccaa tgagggagtt
     2581 tacctgcaaa tggccaaaga cctctggcca caggtccaat caagttacca ggagctgcag
     2641 ttcacgggca aaggaactgt tgtcccggcc gacacgatga tcttttccga aggcgagatg
     2701 agccgcgaca tttatgtggt actcgccgga tctgtgaaga tgatgcgtat tgtccatggc
     2761 gatgaattca tcatcgatgt tcttgggcca ggagaacttt tcggcgagat ggcgtttatc
     2821 gagaacgctc ctcgtatggg ctcggctatc accacgcagg agtcaacact gatacgcatc
     2881 aagcccgaac agctggtatc gagcgtgggc gaaggcgtac tacagaaaat cttcgagaac
     2941 atggcgcggc gcatctggtt ctcccaccag cgcctgatta ttttcaagat acgcgacccg
     3001 cttgtgcgcg tctacgctta tctctataac ctggtacgcc accagaacct gcgcaccaag
     3061 aaccgttcgg cagaagataa gtcttaccgc ttcgatatca ccatgacaga gctcaagacc
     3121 ctgtgtggta tcttgcggct aaccgaaagc accgagcgcg cgctgcacga gaaccacaac
     3181 ctgatctttg acgacacgag catcacgatc aagagtcgca agcggctcga agaccagatc
     3241 gcctactacc gcgccaagtc aggccagatc attgccgaga cgaaataaca ggagaaccgt
     3301 gatgagttcc ctgcgggaac tcatcacggt tctttccagg tggtgcgtac gtcgagcgac
     3361 agtaacagaa tcgacgagtt gtagaaatcg ccgactgaac cgagatagaa ataataccag
     3421 cggtggctga cggtgaaggt aatcggcgcc gctacagtaa aaattactga attggcaatt
     3481 tcgatgcggt gccgccagaa atcctggtag gtgcgcgaag agaacgagtc gaacgccagt
     3541 ttatactgca tgttcgtgta gaatggccaa agcacgccga tgctcgcctc aaaaccccag
     3601 ttaggatcat tcaccgggtc gagtatttct ttttcgagac cgccaccgag ccggcccgta
     3661 aataccttgt aattaaattc ggcaccgaca gtttcacgca ggaaacttgg ccgctggcca
     3721 tttacaggta cgacgaccgt atcgagacgg ttcttctggt aaggccgcag gtaccattcg
     3781 gtgttaaacg tgtacgttat gtcggcgcgc aataggttgc ggataatctg gtccatctgc
     3841 cgcacgtaga agatatacgg gttaaaagca aaggagtgcc tgcggttata gagcataaaa
     3901 ttcaaatcgt tttccagtcc ccacttaaaa tacgaatctg ctgcctgtcc ggggggagta
     3961 tcgatgttgc tggtattgac caccctgccg gtctcataga cgttggaaag gttgagcagc
     4021 gccaggaagc ggaaacggct gtccgcatac ttatcgggtt cggtgatttt ttcctctagc
     4081 gttcggctta ccgaatcact gattcccagc cactggttgc gacctgcggt gcgccggtcg
     4141 ctgcgcatcg catactcata accacgctgc gtcgcgcaga tacggtagct gacattatcg
     4201 cgaatcgggt aaccggtcac cgactgcgca ttcattcctg aaaaaaccag gtcggggttc
     4261 ttccggtaaa agtcgcgtaa ttcagcgcct gtcaggcgaa acgtaaagat atcatagtcg
     4321 ttctgcacgg cgtagcggat atcctgctgg tttttaactt ccgccgcttc gtcggcgtca
     4381 aggtcgtcgt cttcaacaaa ggcaacgtcg cagccgaaag aagcacgcag cgaagcggca
     4441 gcaaactgcg gcgtcaggcg gaatggagta aaagatactt ttccagctcc atccccctca
     4501 gaggtagcat accatttttg ccagagtccc agcctgcgtt caaaggtcgg cggcaccgaa
     4561 actgaatctt ctgcactttc caggtcgacg atttcgcgtt cggcgagcac ccagcgtttg
     4621 ccttcctggc gcaacacgat gcgcatcagc ggattttctt ttggcatggc aatgacccgc
     4681 cgcccgtcgg gtagttcgac ctggcgcacg gcttctttgc cgagacttcc ctctgcatcg
     4741 ccgccgcaga gaagaatatc gatctgtgga tttttcgaca atagcactat gccatcgcgc
     4801 agaggcacac cgctcatcgc tatcgtcaaa tcgggttttt cgagcgcaat ctgcgcgagc
     4861 ggtgcagtca gcgtcgcctc gggtgggtcg acatggagag cgagcaactg cgcctcggca
     4921 taacccacaa gcgacttatt cgaggtaata ccgagtagcg cagtcttgac tccgcctttc
     4981 tgcaaaattg cgtagggttc gaagaacggc gacttgtcgc ggatgatatt tgccgaaagc
     5041 aaacggatgc cacgcgcgcg cctgatataa tcgaggctct cggcgccgat attcaggtcg
     5101 cccgccgcaa caaggccggc gtcgagccgc agcatctgca ggtaatcggc agtaagcgaa
     5161 ccaaagctaa agcgcgataa cctgccggga aaaaaagcgt tacccagatc gagatggtac
     5221 gcggccggat ttttttgctt cgcgctgcgc aggtacgctg caaggcgcat gagttcgttt
     5281 tcctgcagtt cgttattaag aggaaaacgg ccgcccgtgt tcgctgtcag gtaaaaagtc
     5341 agctctttac ccgccgcgcc gagcgaaagc atcggcaata gcagcagcgc taagagtctc
     5401 atggtttttc cggcgtactg ccgagcttct ggcgcagctc gccgagctgc tgggtcaact
     5461 gcgcttcggc tgcgcgcagc tcggccactt tgcggcggag ttcgtcggtt tctttttctt
     5521 tctccgcaag acgctgcctg agatcctgga tttctttgcc gagcgcaaac tgcttttctg
     5581 cgttttcccg gccgagcttt tctttctctt gcgctgactc gagctgcaca cgtgaaaggt
     5641 cttcctgtgc tttgcgcatc tcttcggccg atttctttgc cagctgttcg ttaagggcaa
     5701 ccagctggcg aatctttccg ccgctctccg attcgagaat accaatcttc tcgttaagat
     5761 tggcgatggc gccttcggcg gatttcacct gtgccgtcag cttgtcccgt tcggcagaaa
     5821 actccgcctg cttcttctgc tgttcgcttt tttgcagctc tgcatcgcgg ttcagcgcga
     5881 gattctcttc acgcaatacc tggttctcac gttcgagcag cgccgtcttc ttgcccatga
     5941 gcgctgcttc ggcggcaatc tggtcaggcg tacgttcacg ccgggcaaat acgctgcagc
     6001 cagaggcggc aacgcaggta gcggcaagaa tgggcagaac aagaagagag cgcatgatga
     6061 tgatgcattg gtcaggcgcc gggcagcgcg tcaacttttc agatatttag atcaacttat
     6121 tgtccttgaa atgtaaatcg tgtcgcgatc ccaaacggca atgttcttga cgcggtgtgc
     6181 aaaagcgccc gtcgagatgc tgtaatggaa aatagcggca aaaagtctct caaggaggca
     6241 atggcgaaca gcgaaacaat ccgcatgttc aagtgggatt ggatggagcg cctgtcgcat
     6301 gtacaccccg cgacgccttt cgtcatctat atacctgtag tggtaatcgc catgtactac
     6361 gcggtcctgc agggcaacct gacaattgcg cagatggtag ctgcctttgt gggcggcatg
     6421 ctggtctgga cgctgatgga gtacctcatc caccgcttcc tgtttcatat tcctcagaca
     6481 aaccgcgttt ttaaggcgat ctatttctat tctcacggta tacaccatga cgccccgaac
     6541 gatgcgacgc gcctggtaat gcctccgagc gcttctatcc cgcttgctat cctgttttac
     6601 taccttttcg agggtgttgc gggcatctac aacctgcctc tttttgcggg cttcctgact
     6661 gcctacatgt ggtacgattt catccacttc tcgacgcact tcttcaactg gaaatgggca
     6721 tggtttcgca agatcaaaga gaaccacatg cggcaccact tcctgaccaa taagtacaat
     6781 ttcggcctga gctcaccact gtgggattat gtcttcttca ccctgttcaa gggtaaagcc
     6841 agcctcggcg acaagcaata gctatccatg cctgggcgct gccatcagca gggtctattg
     6901 gcaaaataaa ttgacattct gtgcatgaat tggggctctg agttaagtct cagggcatcg
     6961 ggggtcgata catgaagaga gtaaatcccc cgggttttga atttgcaaat tagagaggaa
     7021 cctatgttag tgaacatgaa acgcaccatt aagatcgggt tcgtgatgct tgtcgcttca
     7081 gcttcgtggc tgattgcaca agatctgaac caacctgcgc ttgattcagc agataaagct
     7141 gctcaggacg cagaaaaaaa agtcgatgaa gcggaaaaag ccgcagaagg caaagtagaa
     7201 gtaccagcta cttcagagcg tgcagcagaa aaacctgctg gaacccagcc tgcaacggca
     7261 gacaaccagg tcggcaacat ctacaacgat ggcaaaacaa cctacgcgac atcgcaggca
     7321 aagtttgagc tgactgcaac ggacaatata tcgaaccttg acttcattga atacaaaatc
     7381 aacgaatcat accagttcag cgtatatggt cacccgttct cgctcgaaaa agaaggccca
     7441 cacaagatta cctaccgctc ggtagaccgt gttggtaacc gcgaagaaga caacatcttc
     7501 aacgtgatca tcgacaacac tggtccatac atgaatttcg cgctgaacaa agcgccaatc
     7561 gtgatcgacg gcaagcgtta cctcgccccg ggtacgaaac tcgaaatccg tgcaaccgac
     7621 aactacagcg gtgtaaaaag cgttgaatac tcaaccaacg gttctgactg gcagccatac
     7681 aaagacggca tcgttctcga caaaccaggt gctgcgcagc tgaaataccg cgcaattgac
     7741 aacctcggca acaagtctga gaatctgagc ggtttctcta caacaactcc tggtcaaggt
     7801 gcaagccccg aagacctcgc ggttatcaac tcactctacg ttgaaaacac tcctccggta
     7861 gtgatgatca ctcctgcaaa acgctggatc aacgttgatg gcaagaaata cgcaatgcgc
     7921 gacaacacct acctgatcga agcacaggat gctgaatctg gcgtggcaaa catcttcgtc
     7981 aaagtagaca acggcgctga atggcagatc tacagcggca agctggcttt ccagaccgaa
     8041 ggtcctcaca gcatcgaagc gaaagcggta gataaagttg gcaatgaatc gcagccagta
     8101 aaggttgagt tcatcgttga tgataacccg ccaaaatcaa caatccgccc ggttggaaca
     8161 actgctgaaa aagcagctga gccagcaccg gcaccagcac agtaaggtta agaccttgct
     8221 gacacgaagc cccgcaattg cggggctttt tttttccctc gttatctcgg cagctgaggc
     8281 acctcaacta tctgcctcgg ggcaggcgta ctttcgtcgc ttcgtcgaac aattcacccc
     8341 gctggtggta cgcaacgaaa aacgcgaccg caggctgaat ttccgctggg acacgacaaa
     8401 cagggactgt gccggtcttg tgcgctacgt ctttcatgaa gcattgtcac aacaccgtga
     8461 atcgtttttc acgcactatc ctcaaatggc acgactgggc aactatgcag gcggtgcgga
     8521 gttctcactt gcgggacgct actggagcga aggaaaccag actgcaccgg atatacttcg
     8581 ccatgcgcgc tacgtgggcc gcgagacggc agaaccgaag ctgaagactg gtgacatact
     8641 ctattatgaa tccgctgaat ggcgcatccg gcatgtgatg ctcgtggttc gctcgggcaa
     8701 aagcgtcttt ctcgtctacc atactggcga caaacgggat gaactgcggt tacggacggt
     8761 tgatgatatg cgcaggcagg atgaggtgca gtggcaccct gcacccgata atcctgtttt
     8821 tcgcggcgtc taccgcccga attttctcca gtaaccatca gggttatcag gctgcaggtc
     8881 gcaaatgatt gccgtgccat gtcagtctgg aaagtgagat tatgttcggt attcgcgcgg
     8941 cctggctggc tgtcctaatt gttgctcttg cggcatgtgg caaaaagaaa ctagagaccc
     9001 ctgaggcagc gctgtcggca gcgcaggaag catacctgaa gcgcgacgcc aaggcattca
     9061 ttgccgtact gtcgaaagag accctgtcag aactcgaaga aaaaattgaa tcgatgcgga
     9121 ccatgttcca gtccatgccg gaaaatccac aggcggcgaa ggcctttgaa cacatggcaa
     9181 agcagatgaa catgcctgtt tcgaagctca aagagctgac gattgaagac tttatcggct
     9241 acatgatgca gaatgaagct gccagcggca gcgaaacgac tcttctgcct aaagaaatgt
     9301 tgccaccggc gaaagttgtt cgccgcaccg aaaagggcaa taaggctaca ctctacttcg
     9361 aagacgatga gaagctttca tttatcaaga ctgacgccgg ctggcgtgta cacctcgatg
     9421 cagaaaatgt cgctgcgaag ggtatgccga tgcagggcaa cgaagagcgc gaagagtagt
     9481 actgcatacg gcgctgccag cgccgcaact tcataagatt ttcagggacc ttcgagcgat
     9541 tcgagtgtaa tagaaccgtt ggtgtaaacg cagatttcag cggcaattct cagtgattcg
     9601 aggcagacct cttttgccga gagatgtgta ttcttgacga gggcccgggc ggcagccaga
     9661 gcgtagtttc cgcccgaacc aatcgcgacc acaccctctt caggctcgat gacatcacca
     9721 ttgccggtca gcaggaatat cttctcctgg tcggctgcaa tgagcattgc ctcaagccgg
     9781 cgcagcatct tgtctgtccg ccagtcctta gccagttcta ccgccgcgcg cgtcaggtta
     9841 ccgccaaagg cctgcagttt ctcttcgagc ttctccagca gggtaaatgc atcagctgtg
     9901 gcgcctgcga aacctgtcag tatcttacca ttgagcaact tacggacctt tacggcattg
     9961 cccttgagga tggtttggcc tgcactcacc tggccgtcgc cggccattgc tacctggttg
    10021 ccgcgtttca cacagagtat cgtggtagcg tgtatttgca taagtataaa tccgcaacaa
    10081 gcctaccaaa gcgcgaggtg atcttcggtt acaccaaacc cggtgaactt ttccgtttct
    10141 tcgccaatct tgacctggcc gtcaaatgtg ccgtacggct gcacgaactg gctgcgcaag
    10201 agccccgcat tgaggttttc gctgcgggca ccaaatggcc tgaaacgcgc ttcaaagaca
    10261 ttgtcgcggg tcagtatgcg ccagatagat ttctctgccg ggcgaatata gtgaaaatgc
    10321 gcagtgctga gcgccatcgg tttgccatcg agccagagtg cattttcaat gccctcgttg
    10381 aacttgtcga cgaggttcac cgcgagcgat ttgcctgact cggtcttgcc gaccatagac
    10441 aaccaattcc actcggtgcg gcgcggaggg taccctttgc tgtagtccag aatgccgaaa
    10501 tcccccgaaa acttttgccg gcggccctgc ccgataaatt caccatctac tgccatggcc
    10561 atttctttgt aggtgaagtg gaaaggccgc atgctgtcag aaggagctat agtgctgaga
    10621 ccgttatggc gcagcgtgaa ggtggccgag agatcaaact ggccgcgcat attaaaaaca
    10681 atcttattct gcgtcggcag aatttcatat ggtcccaggc gccacgtatc attcagtgtc
    10741 gcatcaaaat cggtagcgaa gcccatgggg cgcgtctggc gatcttcgat aaagaacttc
    10801 tcagaaggga tataaacata actaaacgca gacgaaagat agcctgcgtc gacaattgcc
    10861 atgcccgcgt acaaatcacg gctaaacaca ccgagataaa tccatgcctt acgctcggta
    10921 cggcgccggg ggaaaagctt gctgttgaaa tcccattcat gggtttttgt gtcagcaatc
    10981 gggccttcgt ataagccgaa tatgcccggc tcaccgggaa caggaagtcg ggcacgctgc
    11041 ggcttgaagt ctgacatgga ctatttttgt tgcatgaggt acgagtgcac ggcattggcg
    11101 cacttgcgac cttcagcaat tgcccagacg atcaggctct ggccacgacg aatatcacca
    11161 gcagcaaaca ctttgtcaat ggtggtgcgg tgtgcgccgc tgtggtcgcc gaaatcagcc
    11221 ttcacattgc cgcggttgtc gagagtcaac ccaagctttt caaactgctc gagcagaccg
    11281 cccttgaccg gccccacgaa gcccatggcc agcagtacga gctcggcttt cagttcaaac
    11341 tcagagccat cgacatcaac aaacttgccg tctttcattt caactttttt gcacgaaagc
    11401 gacttcacat tgcctttgcc atcgtcgttg aagcgaactg tattgatcga ccacatacgc
    11461 tcagcgcctt cttcgtggct cgaagacgta cgcagtttca tcggccagta tggccagggt
    11521 gtcgaagctg cgcgctcaag cggaggcata ggaaagagtt caagctgggt caccgacgca
    11581 gcaccgtggc ggtttgaggt accgacacag tctgaccccg tgtc
//
LOCUS       DAOQJF010000064        11568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2809_length_11568_cov_5.397637, whole genome shotgun sequence.
ACCESSION   DAOQJF010000064 DAOQJF010000000
VERSION     DAOQJF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2809_length_11568_cov_5.397637"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLF85_05105"
     CDS             complement(<1..219)
                     /locus_tag="PLF85_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19071.1"
                     /translation="MLSEQMQQVLAAYNEGLALYKKREFKMAAEMFKKALAIKKDDGP
                     SQVYLDRCIHFIEEPPPADWDGVYVMKTK"
     gene            307..699
                     /locus_tag="PLF85_05110"
     CDS             307..699
                     /locus_tag="PLF85_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19072.1"
                     /translation="MRKAAFITLFFLGSLNAASLSSLHVDQPQSHAVQCEPASARPDC
                     HNDLPHEHDATHAGEESLFEYIFQVSREAPVIGLAFFLVPDCVRRVNLVSPALLFSAP
                     VPAYAGLPLSSPVPHAVQSRAPPGSPQV"
     gene            733..3975
                     /locus_tag="PLF85_05115"
     CDS             733..3975
                     /locus_tag="PLF85_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNE19073.1"
                     /translation="MAEKFVNYILARRVIITISAALLFGAAVYVGTELPVDAVPDITN
                     VQVIVNAKTGGLDPQQIEKSVTYFVEAEMAGIPGVREVRSLSRFGLSQTVIIFEDGTD
                     IYWARQQIAERIAKVGRVVPAEVQLMMAPITTGLGEVFMYTVLAKEGSTLAKKEERDR
                     LTHLRTIQDFVIRPYLKSNVPGVADVDSLGGYEKQLHVEIDPLRLDALGLTFDHVRRT
                     LVNAGENFGGGYIESQSQQLIVRTNGAVTPESLLNIPQRIGWQGGFVRLKDFAHLHQY
                     HTQRVGAATYRGEETVLGTVLMLAGQNSRQVARDAELALAHVPLPDDVRVEVVYSRSY
                     LVGATLGTVAKNLGEGAGIVVLILLFFLGNFRAALFVALAIPLAFAFALVGMRLSGIS
                     ANLMSLGALDFGLIVDGSIVLIESILSKMELQQPQTRAEKLQIAKTATLEVLKPMTVG
                     MFIIMLVYVPILLLEGVEGKLYYPMAVTVIFALAGALLVSMILMPVFGSMLTAKPHSG
                     KSGIFSRVYSLYTPMLNFPLRHIQKILPGVGAFLLVCTFLFFRLGSDFMPALNEGDMT
                     INLLHDARISLTESIRREKQAEQIILGFPEVHAVFGRIGTSEAATDPMGVNLTDLFVV
                     LKKESSAWRRNARGKVITKEELFAEIEQALRPVLQVTRGLAESEITLTQPIAMRFNEI
                     LEGSRADVSLRIYGRDLENLFTLQEKAVEILHSVKGAKEVTLDALTALRQSTIVEAKP
                     RQDVLNYYGVAINELSETFQTAMVGRVVGSFYDEDWRFPIVIKLADESREKISHLERI
                     PIGLPTGGTLPMKQFADFGRRETVTSIARSGLNRYAGVAVYLGERDTLSYVREAQEKI
                     QRELPLKSENRLQWGGQFRNLQRARTRLMLIVPVVLLAIFYLVWRVFGSWRQTLLIFL
                     TIPFAWTGGVIALLLAGLSFSVSAAVGFIALSGVAVLNGLVKVSYLNQLVGEGHALEE
                     AVRIGAVARLRPVLMTAAVASLGFLPMAINTGIGSEVQRPLAVVVLGGLVTATLMTLL
                     LLPAFYLWGERQNQSLQPLRAAAVQPVTEVIAEEEPAAAPQPKKKRGRRS"
     gene            complement(4003..5877)
                     /locus_tag="PLF85_05120"
     CDS             complement(4003..5877)
                     /locus_tag="PLF85_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNE19074.1"
                     /translation="MLRDTTAFTAPQNRSLRLAAKTLVLLAASLLFSCTEHKMPKGAD
                     YGRLSKLFTITAPPWARKHAEMPQFSGEQQNQLLAWLAEGKIEIDPPEQIADFEYVNS
                     LEKFLTTGEAADRVTVLTATRIEAPENLIPTQLEIFWGNVVPKVIDSLTPGSRFSACS
                     GKILTAHSGGKHYILDGHHRWAACLFLRRFVDHAEDYRRLASPYRYYEERKIYELLSA
                     ATSQKREIPLQVEVQSLEGNPQGIARALFEAAKLGHGRFDRFDTGETPNAAMTYLQNT
                     MLLDSTLLQWLYFAAIILASLIASRLLIAVLQFRQKNNGAVDETRPTLISVALETMRK
                     YIYTLAFLLAVKKGLVVLTLSAEALRTVNTGLVVAVIWLATIFAARLFARFMERWQEK
                     ITTQDKAAEIVHLFPLLIRVGKLLIYIFGVLLILNRVGYNIYSAIAGLSVGGFALAMA
                     GREAVGHIFAGVSLYLDKVVKEGDYLLLPSPIDTWGRVEKVGIRSTTIRTKYNSLLIV
                     PNSLLANQQVDNVSAGGRKRMFRGKLVIAADTPAEKLEQAISAMRRMLAETEFVRDGD
                     VHFMKFDTLGFYVRIQFFVEPFNHYHDTVHALNLKILRWLESNGVRVAVNLDALAKRS
                     "
     gene            5990..8071
                     /locus_tag="PLF85_05125"
     CDS             5990..8071
                     /locus_tag="PLF85_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE19075.1"
                     /translation="MQLRGVKALASLPESELAEIEPYLECREYQPGSEIVHFGSKADD
                     MYLVLSGSARMSRGVLELGSLTSGDHFGELGLISNRPRAATIVAESVLSLARLTRERF
                     TDLQREKPAVAVKLMAGLINTLGRQLVDRTESLDLLLRQRSLPRQATVAIKRENGTIQ
                     EVKTGSVLSAILPERIGDVPVVAALVDHRVSSLDTQIFSDVAVEPLTVKHWEGERVLR
                     HSLALLLMEAASEFEPPIRLRLGFAVGPAQWVHVPKSAQEQLQELADKLTRRMRELIA
                     TNADFREEWWSIDEAISYFQAQEFGGTVQLLKTFHSLTTPLVTCGRIFALYNGPLLPH
                     AGLIEGFRIQQSNHDLILLSGQDTEVPEGFEPFARLSEDSSHWLRSFELTSVGELNRA
                     CVDGRVGEVIRVAEGYHEKRLAQIADEIVARSGVKIVCVAGPSSSGKTTFVRRLSIQL
                     RINGLVPEGISLDDYYLDRDQVPLNDRGEKDFETLRALNLPLLAEHLRTLLEGREQAT
                     ARYDFRTGMCDKTGGRIVQLTEQKLLVLEGIHGLNPELLSGVVPPENVFRIFIQPMLT
                     LPIDTVSHINPSDVRLVRRIVRDRRQRGFATADNIRRWGDVKAGEHAYIFPFVPEADA
                     VFDTSLIYELSVLKVYADRYLLEVPQDHTAYATAFRLRQLLDQFVAISPDDVPSTSIL
                     REFIGKGSFEN"
     gene            complement(8068..9219)
                     /locus_tag="PLF85_05130"
     CDS             complement(8068..9219)
                     /locus_tag="PLF85_05130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE19076.1"
                     /translation="MEIRIADSFSAVLADAWNTLVEKTNPFMRHEFFAALENTRCIGK
                     GTAWQPLLFLAFDPQLVAAIPAFVRSDSYGEYIFDWAWANAYAQAGLPYYPKLTIAAP
                     FTPATGRRLLGEPGEAVQAALLSALVTESERRGVSGIHVLCETRDEQRMLSQHTFAPR
                     LTHQYHWINRDFHTFDDYLAGLRAHRRKEIKRERRKAAELGLEIRTLTGDAISEADML
                     AMYNFYTETYARKWGSPYLNRDTFIELHQTMRNFLVLVLAYDGNKPVAGSIAFRAADR
                     LYGRYWGALAYYPFLHFELCIYRLIDFAIEQKIGLFEAGAQGEHKFARGFTAMPVCSS
                     HRLFHPQGSAAIEDFLRRERRATARAIHAYRAASPNKDEPEYVPELEER"
     gene            complement(9278..10099)
                     /gene="ygiD"
                     /locus_tag="PLF85_05135"
     CDS             complement(9278..10099)
                     /gene="ygiD"
                     /locus_tag="PLF85_05135"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNE19077.1"
                     /translation="MPTLSSGALFLNNPNRLPAFFIGHGSPMNALEKNAFTAEWRNMF
                     TGLTGVRALLMVSAHWVTHGTQVTAMPQPRTIHDFGGFPRELYQVSYPAPGSAEIAQE
                     VIAALEPRPAKADTSWGLDHGTWSILVHALPDAKIPVLQLSIDGKASPADFFAIGRSL
                     RPLREQGILLCGSGNIVHNLSRVDWSRLDETGFAHDWAKEAAEISRGYLLNRDWQKLV
                     DFPALPQSMQIAINTAEHYVPLIYMLGAAYEEENLRLFNDTPVGGALTMTSVQFG"
     gene            complement(10062..10334)
                     /locus_tag="PLF85_05140"
     CDS             complement(10062..10334)
                     /locus_tag="PLF85_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19078.1"
                     /translation="MKEKSHTILAGRCREKLSDTSWDHLLAERIQSARIQQTRSRTLW
                     FSASLLVVAGAITLTAWSYNEAQTNAGMMAMIDEVAYPVVGSAFSE"
     gene            complement(10360..10851)
                     /locus_tag="PLF85_05145"
     CDS             complement(10360..10851)
                     /locus_tag="PLF85_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE19079.1"
                     /translation="MDFAAIIAETKRPVLAAIRRYLDARFAYAIDDVAQEVYLRAYRA
                     LTKGKLQDSSKLRSYLYTIARNEALRMNARGEREEKKAEKFLNARREKLSVSSDTVST
                     RTMTGEVAEALADIPEHYAQVVQLSMLGFSAREIVEKLQIRPGTVKSRLSRGLALLRE
                     KLA"
     gene            complement(10864..11310)
                     /locus_tag="PLF85_05150"
     CDS             complement(10864..11310)
                     /locus_tag="PLF85_05150"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNE19080.1"
                     /translation="MKSHKYKKLFVVLTLISLAGMNACRPWKHKSPEERAEWMVKRIS
                     KELDLNDTQKQTLNRIKEEFIAKHKTDRPQMEAQFAALSDLVRADTIDQAKLKDLRRK
                     HQAHRESMENFFVEKATEFHKVLTSEQRVKAAGLMQKYFRKFAGDK"
BASE COUNT         2292 a         3202 c         3372 g         2702 t
ORIGIN      
        1 tttcgtcttc attacgtata cgccatccca gtctgcgggg ggcggttctt cgataaagtg
       61 gatgcagcgg tcgaggtaga cctgtgaagg gccgtcgtct ttcttgatcg ccagcgcctt
      121 cttgaacatt tctgctgcca ttttgaattc gcgcttcttg tacagcgcaa gcccctcgtt
      181 gtaggcagcc agcacctgct gcatttgttc ggaaagcatg ggcatttata cgaacaaaag
      241 ggggattgta aagataaaaa gaattccttt tgacgtctta tcctgtccag cggttctgct
      301 gttgcagtgc gcaaagccgc gtttatcacg ctgttctttc tggggtcgct gaacgccgct
      361 tctttatcct cattgcatgt agaccagccg caaagccacg cggtgcaatg cgaacctgca
      421 tcggcacgcc ccgattgtca caacgatctg ccgcacgagc acgatgccac ccatgctggc
      481 gaagaatcac ttttcgagta catctttcag gtaagccgcg aggcaccggt aattggtctt
      541 gcgttctttc tcgtgccgga ttgtgtccgg cgcgttaacc ttgtttcgcc cgcgctgctg
      601 ttttctgccc cggtacccgc ctatgcgggt ttgccactgt cgtctcctgt accgcatgcg
      661 gtacaatccc gtgctcctcc aggttcccca caagtttaga tcgaggcctt gggccgtttg
      721 taagggggaa taatggctga aaaatttgtg aactacattc tggcgcgccg cgtgattatc
      781 acgatttcgg ctgcgctact ctttggcgcc gctgtttatg tcgggactga actccccgtc
      841 gatgcggtgc ccgatatcac caacgttcag gttatcgtca acgcaaagac gggtggcctg
      901 gatccacagc agattgaaaa atctgttacc tattttgtcg aagcagagat ggcgggtatt
      961 cccggcgtgc gcgaagtgcg ctcgctctcg cgcttcggtc tttcgcagac cgtgatcatc
     1021 ttcgaggatg gcaccgatat ctactgggcc cggcaacaga ttgccgagcg tatcgccaaa
     1081 gtgggccggg tcgtgcctgc cgaggtgcag ctgatgatgg cgcccatcac gacgggactc
     1141 ggcgaagtgt tcatgtacac ggtgcttgca aaggaaggca gcacactggc gaaaaaagag
     1201 gagcgggatc gcctcacaca cctgcgcacc atacaggact ttgtcatccg tccgtacctt
     1261 aaatcgaacg tgcccggtgt ggccgacgtt gattcgctcg gcggatatga aaagcaacta
     1321 catgtcgaaa ttgacccgtt gcgcctcgat gcgctgggac tgacttttga ccatgtgcgc
     1381 cgcacgctgg tgaatgccgg tgagaatttt ggcggcggct atattgaaag ccagtcgcag
     1441 cagctgattg tgcggaccaa cggcgccgta acgcccgaga gcctgctcaa tattccgcag
     1501 cgtatcggct ggcagggcgg ttttgttcgg ctgaaggatt ttgcccacct gcaccagtac
     1561 cacacgcagc gcgttggagc tgccacgtac cggggcgaag agaccgtgtt gggcacggtt
     1621 cttatgcttg cggggcagaa cagccggcag gtggcacgcg acgctgaact cgcattggcg
     1681 cacgtaccgc tgccagacga cgtgcgcgtg gaagttgtct attcgcgcag ctacctggtg
     1741 ggtgccaccc tgggcaccgt tgctaaaaat ctcggcgaag gggctggaat tgttgtcctt
     1801 attttgctct tctttctggg taactttcgc gcagccctgt tcgtggcgct ggcgataccg
     1861 ctcgcctttg cctttgccct cgtgggcatg cgtttatcgg gcatatcggc gaacctgatg
     1921 agcctgggtg ctctcgactt tggcctcatt gtcgacggtt cgatcgttct catcgagagt
     1981 attctttcga agatggaact gcagcagccg cagacgcgtg cggagaaact gcagatcgcg
     2041 aaaaccgcga ccctcgaagt gttgaagccg atgacggttg gcatgtttat catcatgctg
     2101 gtctatgtgc cgattcttct gctcgaaggt gtcgagggca aactctatta cccaatggcg
     2161 gtaacggtaa tcttcgcgct tgccggcgcg ctattggtat ccatgatttt gatgcctgtc
     2221 tttggtagta tgctcacggc aaagccccac tcaggtaaaa gcgggatttt ttcccgggtt
     2281 tacagtctct atactcccat gttaaatttt ccgctgcggc acatacaaaa gattctgccc
     2341 ggcgtcggcg cttttttgct cgtatgtacc ttccttttct ttcggctggg atctgatttt
     2401 atgcccgcgc tcaacgaagg cgacatgaca atcaacctgc tgcatgatgc gcgcatctcg
     2461 ctcaccgagt cgatcaggcg cgagaagcag gcagagcaga ttattctcgg ttttcccgag
     2521 gtccatgccg tcttcggccg tatcggaacg agcgaagctg ctactgaccc gatgggtgta
     2581 aacctcaccg acctgttcgt ggtcctgaaa aaagaaagct ccgcctggcg tcgcaacgcg
     2641 cgcggtaaag taattacgaa agaagaactt tttgcagaaa tcgagcaggc gctcaggccg
     2701 gtactccagg taacgcgagg actcgccgaa tcggagatta ccctgacaca gccgatcgcc
     2761 atgcgattca acgaaattct tgaaggcagc cgggccgacg tctcgctgcg tatctatggt
     2821 cgtgatctcg agaacctgtt tacgctgcag gagaaggctg ttgaaattct gcactccgta
     2881 aaaggcgcga aagaggtgac actcgatgcc ctcacggcgc tgagacaaag tactattgtt
     2941 gaagcgaagc cgcggcagga cgtgctgaat tactacggtg ttgcgatcaa cgagctgagc
     3001 gagactttcc agacagcgat ggttggtcgg gtggtgggta gcttctacga cgaggattgg
     3061 cgttttccga ttgtcattaa acttgccgac gaaagccgcg aaaaaatttc gcacctcgaa
     3121 cgcattccga ttggcctgcc taccggtggc acgttgccga tgaagcaatt tgctgacttt
     3181 ggccgccgcg agacggtgac gtcgattgcg cgcagtggtt tgaaccgcta tgcaggtgtt
     3241 gctgtgtacc tgggcgagcg tgacacgctg tcttacgtgc gcgaggcgca ggagaaaata
     3301 cagcgagaac tgccgcttaa aagcgagaac cgcctgcagt ggggtgggca gtttcgcaac
     3361 ctacagcgcg cacgcacccg cctgatgctc attgtgccgg ttgttctgct cgcgattttc
     3421 taccttgtct ggcgggtgtt cggttcatgg cggcagacgc tgctcatatt tcttaccata
     3481 ccctttgcct ggacaggcgg agtgattgcg ctgctgcttg cgggtttgag cttttccgtt
     3541 tccgctgccg tgggttttat cgccctttcg ggtgtcgccg tgctgaatgg tctggtgaag
     3601 gttagctatc tgaaccagct cgttggagaa gggcatgccc tggaagaagc ggtgcgcatc
     3661 ggcgcggtgg cgcgcctgcg cccggtattg atgactgctg cagtggcaag ccttggtttt
     3721 ttgccgatgg cgattaatac cggtatcggc tcagaagtgc agcgcccgct tgcggtcgtc
     3781 gtcctgggcg gtctcgtcac cgcaacgctc atgacgctgc tgcttttgcc cgcattctat
     3841 ttatggggtg agcgccagaa tcagtctctt cagccgctga gagctgctgc ggtgcaacct
     3901 gtaacagagg tgatagctga agaagaaccg gcagctgcgc cgcagcccaa aaagaaacgc
     3961 gggcgaagat cgtagccggt gcgggggttt ttccggaacc ggctaagatc ttttggcgag
     4021 cgcgtcgagg ttcacggcaa cccggacacc gttggactcg agccagcgca gtatcttaag
     4081 attcagcgca tgcaccgtgt cgtggtagtg gttgaatggc tcaacgaaga actggatgcg
     4141 gacgtagaaa ccgagcgtgt caaacttcat gaagtggaca tcgccgtcac gcacgaactc
     4201 cgtctcggcg agcatacggc gcatggcgct gattgcctgc tccagctttt cggccggggt
     4261 atcagcagcg atgacgagtt tgccccggaa cattcttttg cgcccgccgg ctgaaacatt
     4321 gtcgacttgc tggttggcga gtagcgaatt cgggacaatc agcaggctgt tgtatttggt
     4381 acgtatcgtc gttgaacgga tgccgacctt ctcaacgcgt ccccaggtgt cgattggcga
     4441 aggcagcaac aggtaatcgc cttctttgac taccttatcc aggtaaagcg agacgccggc
     4501 gaagatatgc ccgacagctt cgcgtcccgc catcgccagc gcaaaaccgc cgacgctgag
     4561 tccggcgata gccgaataga tgttgtagcc gacgcggttg agtatcagca gcacgccgaa
     4621 gatgtagatc agcagtttgc caaccctgat cagcagcgga aacaggtgca cgatttctgc
     4681 cgctttatcc tgcgtagtga tcttttcctg ccagcgttcc atgaagcggg caaagagtcg
     4741 cgcagcaaaa attgtagcca gccaaatgac tgcgacgacc agcccggtat taaccgtgcg
     4801 cagcgcctcg gcactcagtg tgagcacgac caggcctttc ttcacggcga gcaggaaggc
     4861 gagcgtgtag atgtacttgc gcatcgtttc gagcgcaacg gaaattaggg tggggcgagt
     4921 ttcgtctact gcaccattat ttttctgcct gaattgcagt actgcaatca gcaggcggct
     4981 cgcgatcagg cttgccagta tgatcgcagc aaagtagagc cactgcagca gcgtgctgtc
     5041 gagcagcatc gtgttttgca ggtaggtcat tgctgcgttc ggtgtttcgc ccgtatcaaa
     5101 ccggtcaaaa cggccgtgcc cgagtttcgc cgcttcgaat aatgcccgtg cgataccctg
     5161 tggatttcct tcgagcgatt gcacttcgac ctgcagcggt atctcccgct tctgtgacgt
     5221 ggcagcactc agaagttcgt agatcttccg ttcttcgtag tagcgatagg gcgaggccag
     5281 gcggcggtag tcttctgcgt ggtcgacaaa gcggcgcagg aacaggcagg ccgcccagcg
     5341 atggtggcca tcgagtatgt agtgtttacc tccgctgtgc gcagtcagta tcttgccgct
     5401 acaggcgctg aaccggctgc cgggcgtcag cgagtcgatg accttgggaa caacgttccc
     5461 ccagaaaatt tccagctgcg tcggtatgag attttcgggc gcttcgatac gcgtggcggt
     5521 caaaacggtc acccggtctg cagcttcacc tgttgtgaga aatttttcca gcgagttgac
     5581 gtactcgaag tcggcgatct gttcgggggg atcaatttca attttccctt cggccagcca
     5641 ggccagcaac tggttttgct gttcgccgct gaactgcggc atctcggcgt gcttgcgcgc
     5701 ccacggcggg gcagtgatcg tgaagagttt gctgaggcgg ccgtagtccg cgcccttggg
     5761 catcttgtgt tccgtgcagg agaagagcag cgaagccgcc aatagaacca gcgttttggc
     5821 cgccaggcgc agcgaccggt tctgtggagc ggtgaaagcg gttgtatctc ggagcacgaa
     5881 tgcacacagt ggctgctcaa tcccgtgtaa aagacttttg cccaggtgac ccttgcagct
     5941 caattccatt tagtcgccgc tctgtctgca tctgtagatc tggtgcagaa tgcaactcag
     6001 gggagtcaaa gccctcgcgt ccttgcccga aagcgagctc gctgaaatcg agccatacct
     6061 cgaatgccgc gaataccagc cgggcagtga gatcgtgcac tttggcagca aggccgatga
     6121 catgtacctc gtgctgtcgg gcagcgcgcg tatgagccgc ggcgtgctgg agctcggcag
     6181 cctcacgagc ggtgaccact tcggtgaact gggattaata tcgaaccgcc ctcgcgcggc
     6241 gacaatcgtc gctgaatcag tgctctcact cgcgcggctg acccgcgagc gcttcaccga
     6301 cctgcagcgc gaaaagccgg cagttgcagt gaagctcatg gcgggcctta tcaatacgct
     6361 ggggcggcag cttgtcgacc gcactgaaag cctcgacctt ctgctgcgcc agcgttcgtt
     6421 accacgccag gccacagtcg cgatcaaacg tgagaacggg actatccagg aagtgaagac
     6481 cggatcagtg ctgtcggcga ttttgcctga acggattggc gatgtcccgg ttgtcgccgc
     6541 acttgtcgat caccgggtga gctcgctcga tacacagatt ttttccgatg ttgcagttga
     6601 acccctcacg gtgaaacact gggagggtga gcgcgtcttg cggcactcgc tcgcgcttct
     6661 gctcatggag gcagcctcgg agtttgagcc accgatcagg ttgcggctgg gttttgccgt
     6721 tggtcccgcg cagtgggtgc atgtacccaa gtcggcgcag gaacagctgc aggaactcgc
     6781 cgacaagctc acgcggcgaa tgcgcgagct cattgccacc aatgcagatt tccgggaaga
     6841 gtggtggtcg atagacgaag cgattagcta tttccaggca caggaatttg gcggcacggt
     6901 gcagctgctg aagactttcc attcgctcac gactccactt gttacctgcg gtcgtatctt
     6961 cgcgctctac aacgggccgc tgctgccgca cgccggactc atcgaaggtt ttcgtatcca
     7021 gcagagtaat cacgatctga ttctgctgtc ggggcaggat accgaggtgc ccgaaggttt
     7081 cgagcctttc gcgcgtctgt cagaagacag cagccactgg ctgcgctcgt tcgagctgac
     7141 cagcgtgggg gagctcaatc gtgcttgcgt cgatggccgt gtgggcgaag tcatccgtgt
     7201 tgccgagggt tatcacgaga aacggttggc acagattgct gacgagatcg tggcgcgcag
     7261 tggtgtaaag atagtctgcg tcgccggtcc atcgtcgtct ggaaaaacca ctttcgttcg
     7321 gcgcctttcc atacagctac gcatcaacgg gctggtaccg gaaggtatat cgctcgacga
     7381 ctattacctt gaccgcgacc aggtgccgct caacgatcgc ggtgaaaaag actttgaaac
     7441 cctgcgcgcg ctcaacctgc cgctgctggc cgagcacctg cgcacactgc ttgaaggccg
     7501 tgaacaggcg actgcgcgct acgatttccg gaccggcatg tgcgacaaga ccggtggtcg
     7561 cattgtgcag ctcaccgagc agaagctact ggtacttgaa ggtatccatg gcctcaaccc
     7621 cgagctgtta tctggtgtgg tgccgccgga aaatgtcttc cgcatattta ttcagccaat
     7681 gctcacgctg ccgatcgaca cggtcagcca catcaaccca tccgatgtgc gcctcgtgcg
     7741 gcgtattgtt cgtgatcggc gccaacgtgg atttgccacg gcggataata ttcgtcgctg
     7801 gggcgacgtg aaagcgggag aacacgccta catctttccg tttgtgccag aggccgacgc
     7861 cgtatttgat acctcgctga tatacgaact gtcggtgctg aaggtctatg ccgaccgtta
     7921 cctgctcgag gtgccgcagg accacacggc ctatgcaacg gcattcaggt tgcggcaatt
     7981 gctcgaccag tttgtggcga tctcaccgga tgacgtgcct tcaacgtcta tcctgcgcga
     8041 attcatcggc aaaggtagtt ttgagaatta acgttcttcc agttcaggca cgtattctgg
     8101 ttcatcttta ttaggacttg ccgcgcggta cgcatggatt gcccgcgcgg tcgcccggcg
     8161 ttcgcgccgc aggaaatctt cgattgccgc cgacccttgc ggatggaaca gccggtgcga
     8221 actgcagacc ggcatcgcgg taaagccccg cgcaaacttg tgctccccct gcgcgcccgc
     8281 ttcaaagagc ccgattttct gctcaatggc gaaatcgatc agccggtaga tgcagagttc
     8341 aaagtgcaag aagggatagt aggcaagagc tccccagtag cggccataaa gccggtcagc
     8401 agcacggaag gcgatggagc cggcgacagg cttattgcca tcgtaagcca gcacgagtac
     8461 caggaagttt cgcatcgtct ggtgcagttc gatgaaagta tcccggttga gatagggaga
     8521 tccccatttg cgggcataag tctcggtgta gaaattgtac atcgcgagca tatccgcttc
     8581 gctgatggcg tcgcctgtga gcgtgcgtat ttcgaggcca agctctgctg ccttgcggcg
     8641 ctcgcgtttg atttctttgc gccggtgagc ccgcagccca gccaggtagt cgtcgaaagt
     8701 atggaagtca cggttgatcc agtggtactg gtgcgtcaga cggggagcga aggtatgctg
     8761 tgacagcatt cgctgctcgt cgcgcgtttc gcacagcacg tgaattcccg atacgccgcg
     8821 tctttcgctt tctgttacca gcgccgaaag taatgccgcc tgtacggcct caccaggttc
     8881 acccagcagg cgcctgccgg tcgccggggt gaacggtgcc gcgatagtca gcttcgggta
     8941 ataaggaaga ccagcctgtg cgtaggcgtt tgcccatgcc cagtcaaaaa tatattcgcc
     9001 gtacgaatcg ctgcggacaa atgccggaat cgcggcaacc agctgcgggt caaaagccag
     9061 gaacaacagg ggctgccacg ccgtgccctt cccgatgcag cgcgtgttct caagcgccgc
     9121 aaaaaactcg tggcgcatga acgggttggt tttttctacc agcgtattcc aggcgtcggc
     9181 aaggactgca ctgaagctgt cggcgatgcg gatttccatg gcgacaaagc cagttataga
     9241 gatctatagc ctgcgtcgag tgtggtaggc tgcccgttta cccaaactgc accgacgtca
     9301 ttgtcagggc gccgcccacc ggcgtatcgt tgaacaggcg gaggttttct tcttcataag
     9361 ctgcaccgag catgtagatg agcggcacgt agtgttctgc cgtattgatc gctatctgca
     9421 tactctgcgg tagcgcggga aaatccacca gcttctgcca atcccggttg agcaggtagc
     9481 cgcgcgagat ctctgcggcc tctttggccc agtcgtgggc aaagcccgtc tcatcgaggc
     9541 gcgaccagtc gacgcggctc aggttatgca cgatattgcc gctgccgcac agcagtatac
     9601 cctgttcgcg cagcggccgc aggctgcggc caatggcaaa gaagtcagcg ggtgaagcct
     9661 tgccatcgat ggaaagctgc agcacgggaa ttttagcgtc cggtagtgca tgcaccagaa
     9721 tactccaggt accgtggtct aagccccagc tcgtatccgc ttttgccggg cgtggctcaa
     9781 gcgctgcaat cacttcctgc gcgatttcgg ctgaacctgg ggcggggtaa cttacctggt
     9841 agagttcacg cggaaagccg ccaaagtcgt ggatagtcct cggctgtggc atcgcagtga
     9901 cctgcgtgcc atgcgttacc cagtgtgcag aaaccatgag cagcgcgcgt acaccggtga
     9961 gtcccgtgaa catgtttctc cactcggcag tgaaggcatt cttttcgagt gcattcatcg
    10021 gcgagccgtg gccaatgaag aaagctggta gccggtttgg attattcaga aaaagcgctc
    10081 ccgacgacag ggtaggcaac ttcgtcgatc attgccatca tgcctgcatt ggtctgtgcc
    10141 tcgttgtacg accaggcagt cagtgttatt gcacctgcaa ctaccaatag gctggcggaa
    10201 aaccacagtg tacggctgcg cgtctgctga atgcgcgcac tttgaatacg ctccgcaagc
    10261 aggtgatccc agctggtgtc gctgagtttt tcccggcagc gcccggcgag tatggtgtgg
    10321 gatttttctt tcatcttttc tcttttgccg tcatggctat caagccagct tttctctcag
    10381 cagtgccaga ccgcgcgaca agcgggattt tacggtaccc gggcgtatct gcagcttttc
    10441 gacaatctcg cgcgcggaaa aaccgagcat gctcagctgt acgacctgcg catagtgttc
    10501 gggtatatcg gcaagtgcct cggcgacctc gccggtcata gtgcgcgtgc tgacggtatc
    10561 ggaagaaact gacaactttt cgcgtcgcgc gttaagaaat ttttctgcct tcttctcttc
    10621 gcgttcaccg cgggcattca tgcgcagcgc ttcgttgcgc gctatggtgt agaggtagga
    10681 cctgagtttc gagctgtcct gaagtttacc cttggtgaga gcccggtagg cgcgcaggta
    10741 aacttcctgc gctacgtcgt cgatggcgta ggcaaaacgt gcgtccaggt accggcggat
    10801 tgccgccagt accggacgtt tggtttcagc aatgattgct gcgaagtcca cgttgatgcg
    10861 gttctactta tctcccgcaa acttgcggaa atacttctgc atcaagcccg cagccttgac
    10921 ccgctgctcg ctggtcagca ccttgtgaaa ctcggtcgcc ttttcaacga agaagttctc
    10981 catggattcc cggtgtgcct ggtgcttcct gcgcaggtct ttgagcttcg cctggtcaat
    11041 cgtatcggcg cggacaaggt cgctcagggc ggcgaactgg gcctccattt gtggcctgtc
    11101 agttttatgc tttgcgatga actcttcttt gatgcggttc agcgtctgct tttgcgtgtc
    11161 gttcaggtcg agttctttgc taatacgctt caccatccac tcggcgcgtt cttcaggtga
    11221 tttgtgtttc cacggtcggc aggcattcat gcccgcaagc gagataagcg tgaggacaac
    11281 aaacaacttc ttgtatttat gcgatttcat tcgttctggc tccctggggc tgttttggct
    11341 gccccattta cccagttgac cctaatatac aaaaaatggt tccaaaaggg ctttacattt
    11401 tttcgggcaa accggcctat actgtatgcg tattccgctt cgcttgaaat ccccccttgc
    11461 tgccgccttt gtggcaggcc tgcttttcgt gctttcgccg attgaggccg cccagaatgt
    11521 ttacgtgaaa aactataccc cgctgctctc atcgccttca gagcaaac
//
LOCUS       DAOQJF010000065        11557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2816_length_11557_cov_5.483829, whole genome shotgun sequence.
ACCESSION   DAOQJF010000065 DAOQJF010000000
VERSION     DAOQJF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2816_length_11557_cov_5.483829"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PLF85_05155"
     CDS             <1..1065
                     /locus_tag="PLF85_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19081.1"
                     /translation="TLTVAGDIEAENGTVTMTMRLLENDNYLFALEYQNKSGEAKEVG
                     FRLLVDTWAGNTDGVPFLTPAGPGKDSQLHTREFKFTPAYSTLWETIDAEGDGYVYLR
                     NTLTGPGLVAPDQVAFARWGSAYRSEWDYKISSENSILGDSAALLWWQPQRIAAGNAR
                     SVATRFGVFKRTEKTLLDVQDARSGFGYIYLERQNTTATPQDYRVELDSTDADITVPG
                     GKQQQFTLAPKERTVRAIPVSVAGSGKKTVSIKEYQGNAKDPKISTVTFEIPADNGHS
                     ANVPVWLNTRPYPVRYVADREDRKLRCVVKAAAGNQVLGQGELKLLKSNTAQKEFIYG
                     ADIPVPSNYEGETVVEIFNR"
     gene            complement(1193..1573)
                     /locus_tag="PLF85_05160"
     CDS             complement(1193..1573)
                     /locus_tag="PLF85_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19082.1"
                     /translation="MKIRNFLPLALFALLTLPGGLGFLFAWQGFPLVMSLMQVVHALL
                     MVLVFRASLRSAAKNHGAPGAVQRQAYLPLKVNLFLYFVIGQALVWFFADNLTVLQGG
                     YAVQQLGFGFACYSGMLFVSRSSA"
     gene            complement(1570..2094)
                     /locus_tag="PLF85_05165"
     CDS             complement(1570..2094)
                     /locus_tag="PLF85_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19083.1"
                     /translation="MSPDINYAKADELAILIEERFLQLVQEGAFADLEPHLTALAATG
                     NEDQAVTLSLQLRLSDSEREREIIVSETSRAWLADGETMDFNNSDAAVRYLCDGEIRV
                     FQHSSCPHCWGEWEDKHHNPTCPDCGYELGNQVKILMDDSVCPYCLEGKVSRQEPTCS
                     TCGEVVEERYVSWG"
     gene            2198..2686
                     /locus_tag="PLF85_05170"
     CDS             2198..2686
                     /locus_tag="PLF85_05170"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNE19084.1"
                     /translation="MAQTIHEFSAATLGGKEKSLTEYKGKVVLIVNTASKCGFTPQYA
                     GLEAIYEKYKDQGFVILGFPCNQFGAQEPGSESEIEQFCQVNYGVKFPMFGKIDVNGS
                     NAHPLYKFLTSEKPGILGLEAIKWNFTKFLIDKDGNPVKRYAPNTKPEDIAGDIEKLL
                     KA"
     gene            2683..3468
                     /locus_tag="PLF85_05175"
     CDS             2683..3468
                     /locus_tag="PLF85_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE19085.1"
                     /translation="MTPRHWLFSVRDGVATLSLNRPEYGNNINLECLEELRQLSEQIE
                     HNPAVRVVVLRSEGKHSSIGMDVSVIGQMAGQQYEPYAEHLGAGQKCIDAFEAISRPI
                     IAEIKGFCIGAGVILAACADFRISTERAVFALPEVKRSIGVIMGLHRVTRLVGVSSVK
                     RMAMLGENFSARQMLEWGFLTEIVAEEKLAAASANLVKKILALPPLAVTLNKRIADFS
                     YAAFLQQSQQFELREQFQLNQTADFREAIESFQEKRPPSYLGK"
     gene            3504..4400
                     /locus_tag="PLF85_05180"
     CDS             3504..4400
                     /locus_tag="PLF85_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNE19086.1"
                     /translation="MALAACAPPKAKKPATARHILYALPDQLPEKYDYRVQGMQATGV
                     AWLAASFKGDGSLRLQHRDAVELLGRQRADLEQHLLITEASVDEGNRLLNTRAATKRA
                     ANNLREAVVGMPHIRAVHLDFEYNGPGLAEAYVEFVHTLHTALPEPVSLYVTVFPPVG
                     MPQKWSAFFLPEQLAHFADGIVFMLYDLHRNGTAAGCVSPISWLDANVAEFSKLPPEK
                     IWLGAPLYGYQFTGKKATALSKRGFEKIVAQETEADGCRMKKTGASETYYPAPSLYAH
                     YEKLVAKHGFAGITFWRAGLEK"
     gene            4397..5323
                     /locus_tag="PLF85_05185"
     CDS             4397..5323
                     /locus_tag="PLF85_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE19087.1"
                     /translation="MKAMTKLAIIGDAHMQFDEEDVRFFNASDYDAILFVGDLASKNP
                     DSMFRLLPVINGLTKPAFLIPGNHDTTGVRAIIGEVTQNQFLINFGASSQPGRMQRLK
                     AELTGATLCGYSLHTLPGNLQMVAARPFSMGSTNRTINFRPFLEKEYGVSTVEQSRDK
                     IKSLIDQVNSPYIILAHHGPFGLGSKATDMWGADFLPQETDFGDADLAEAVTYAQSSG
                     KAPLAVIAGHMHYPTKHGKKPKQWWAMKDGVLYINAARWPRIFRHEGRLMHHHVQLVI
                     DEQGKSTVCACYTCEGKILPVYDDRALVPEAL"
     gene            5388..6317
                     /locus_tag="PLF85_05190"
     CDS             5388..6317
                     /locus_tag="PLF85_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19088.1"
                     /translation="MKKKLGLLAMMTVLVGILTWYIFPSVFFEPLVNLNRKLSRLTEK
                     TTQISGHTVHYLEGGQGETVVLLHGIFAEKDHWVEFARDLTPHYRVVIPDLPAFGESS
                     RNESEVYDYANQVSRLAELLQKLGISKAHLAGSSMGGTIAALFAVENPQAVTSLAFIG
                     APHGIRSTKQSDMDRMIAEGRAALVASSPAEFDAMMQLLFAKQPFLPYPILHEAKSTA
                     LKNAFSNRRIWNQQLKDRYLLDGIVGKVSAPTGILWGAKDRIFDFSGMTTLREKLPAA
                     KAELATEAGHLPMMEAPGQTAAWYKSFLASQKK"
     gene            6344..7354
                     /locus_tag="PLF85_05195"
     CDS             6344..7354
                     /locus_tag="PLF85_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNE19089.1"
                     /translation="MRTERLAAYFTEKSGTPAKITEFRQLSGGACQDNYLARIESAGG
                     SEDLVLRTDKGGALLGSLTRPQEYQVIERAVAAGVQTPAVKWLSEDTAIVGHPFYLMQ
                     KIEGTANAREILKSRQIDFKRLAAELAMNLAKIHSIEYSPAILPFLDRGLRPDTNDVA
                     LTAVADCRRLLDELPGGYPAIELCLNWAEANAPVTDKVVLAHSDFRSGNFMIGESGPT
                     GPGLTGILDWEFAHWSDRHEDIGYIRMRDWRFGKVKNPIGGFAAPEDFYPHYEAEAKV
                     KLDESKIRFWEIMGNLRWAIGAAQQGERHLSGKDRGIEFASIGRRVAEMELEAMRLIE
                     QA"
     gene            7365..7757
                     /locus_tag="PLF85_05200"
     CDS             7365..7757
                     /locus_tag="PLF85_05200"
                     /inference="COORDINATES: protein motif:HMM:NF040046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HNE19090.1"
                     /translation="MQYRPQNNDLIDAIQEFLLKEVLPFVKENDALAYKTLVSWNMLG
                     VVSRELKNGRPLLEADATLLAELLGEKAEVQTTGDRELWQQAQALAKKAAQKVRAEQT
                     MPGSAEWHAVKKLLTDNLQISNPRYGVE"
     gene            7798..8526
                     /locus_tag="PLF85_05205"
     CDS             7798..8526
                     /locus_tag="PLF85_05205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE19091.1"
                     /translation="MTELYLVRHGQADSAGDNYDQLTETGHEQARLVGEWLAERGYTF
                     TRALHGGLRRQQETLEAIAAAYARHGQDLPAAELHPAFAEFDLRVWAIIAGKLRHGHA
                     EFSELLKQWNRARHENSPDKGDIFKQLTGIILREWVAMGEHFTEAESFPAFRRRVLGA
                     LELQATPATASGKAQRILAVTSGGPIALLTGGALGLSQPKTLGLMRRIYNTSLHALVY
                     DKSQWELQTFNNVPHLPAADRTLV"
     gene            complement(8569..9387)
                     /locus_tag="PLF85_05210"
     CDS             complement(8569..9387)
                     /locus_tag="PLF85_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNE19092.1"
                     /translation="MFNFLSRAGLLVVALTAGISAQARQPFYTETMNFRIEWKGIRAG
                     DVTMRSRIEGNNFLTINAKVNSLPVMHGIYYVQGVFAAKWNFASRSPVIAYEEAYQGD
                     TYQRRKFRFGGRNVRVEKNEKRFHELSYPHSEPVKSQSNETYDVSTSGGYQDLLGAFY
                     YVRSTGKVPKVGEVLRVPVLPAGSRRTLVLKVLGREKRTDVPFFGTREIIHVKSALAD
                     EGKGKQPQGNIFFNTQSQIDMWITADESFVPVKIWTEVPYLGTAYILLENYSQP"
     gene            9463..10188
                     /locus_tag="PLF85_05215"
     CDS             9463..10188
                     /locus_tag="PLF85_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE19093.1"
                     /translation="MSAQKTVLITGATRGIGKGIAIAFADAGYITYGTGRNLSDTSWA
                     TEKKIILKQADVSDQAAMAAIMTEIKEKHGRLDCLINNAGVASNTPASSLGEDEIAKI
                     IDTNFKAVFQCCQSYYKMQRKAGGNIINISSVLGIVGTSLASVYSGTKGAVISMTKAL
                     AIEWANSGFRVNAICPGFIDTDMTEMIKKREAVMQKMLEYIPLKRLGTPEDIAAPALM
                     LASDGAAYITGQIIVVDGGLTAM"
     gene            complement(10228..11004)
                     /locus_tag="PLF85_05220"
     CDS             complement(10228..11004)
                     /locus_tag="PLF85_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE19094.1"
                     /translation="MNKKYDVAATPVPAFKAAADIAEGKRLYISRGCADCHGDDHAGR
                     VFINDPAIGTMAGANLTAGKGGIGKERSDAEMARAIRHGVSKNGRALVFMPATDYTGM
                     TDEDTGKLISYLRSVPAVDREFLPPKAGPLGRFLFLIGEIPVFVSAERIDHNAKPVTK
                     INPTVSVEYGKYVAATCTGCHRDNFAGGPIQGAPPEWPPAQPITAKALSKWNEAQFIA
                     ALRTGKRPDGSMIKFPMPWQSLGKLTDTEMKALWKYLQSI"
     gene            complement(11192..>11557)
                     /locus_tag="PLF85_05225"
     CDS             complement(11192..>11557)
                     /locus_tag="PLF85_05225"
                     /inference="COORDINATES: protein motif:HMM:NF021998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HNE19095.1"
                     /translation="PLAITRHGFLETNGANSIISLPVLCIVLLATEAESNPLLRLWIF
                     FFLGAIFLTNQIHKWAHMPMPPKLPRVLQKLHLILTPEGHAIHHTPPHSGYFCITNGW
                     LNLPLKKLRFFRLIRRFLS"
BASE COUNT         2770 a         3149 c         3223 g         2415 t
ORIGIN      
        1 acactcacgg tagccggcga catcgaagcc gaaaacggca cggttacaat gaccatgcgg
       61 ctcctggaaa atgacaacta cctgttcgcg ctcgagtacc agaataaatc cggcgaagcc
      121 aaagaggttg gatttcgtct gctcgtggat acctgggccg gcaataccga cggcgttcct
      181 tttctgacac cggctgggcc tggcaaagat tcacagctgc acacgaggga gttcaaattc
      241 acgcccgcgt attccaccct gtgggaaacc atcgacgcgg aaggtgatgg ctatgtctat
      301 ctgcgcaata cactcaccgg gcccggtctt gtcgcacctg accaggtggc gtttgcgcgc
      361 tggggctctg cctaccgcag cgagtgggat tacaaaatca gctcggagaa ttcgattctc
      421 ggcgattcgg ccgccctgtt gtggtggcaa ccgcagcgca ttgccgcagg caatgcacgc
      481 agcgtcgcaa cgcgattcgg cgtattcaaa cgcacggaaa aaaccctgct cgatgtgcag
      541 gatgcaagat cgggtttcgg ctatatctac ctcgagcggc aaaacaccac cgcaacacct
      601 caggattatc gcgtggaatt agacagcacc gatgcagaca tcacggtgcc gggcggcaaa
      661 caacaacagt tcacgctcgc acctaaagag cgcaccgtgc gcgcaattcc cgtctcagtc
      721 gcgggcagtg gcaagaaaac cgtcagtatc aaagaatacc agggcaatgc aaaagatccg
      781 aaaatttcaa cggtcacctt tgaaattcca gccgacaatg ggcattcggc gaatgtgccg
      841 gtttggctca acacccgccc ctatccggtg cgctacgttg ccgatcgcga agaccgcaag
      901 ttacgctgtg tggttaaggc tgccgccgga aaccaggtac ttggtcaggg ggagttaaaa
      961 ttgctgaaga gtaatacagc acaaaaggaa tttatttatg gcgcggatat tcccgtgccg
     1021 tccaattacg aaggtgaaac agtggtcgaa atattcaacc gttagacaag aggcgcacaa
     1081 cctccttgtc gcttgtctgc gaaaagtcat cgtactagac accgattgcg cgaaaattgt
     1141 cgggcatgac taagcaggtt actccctcga cctgggtgca cgtcaacgca tctcaggcag
     1201 acgaacgaga gacaaaaagc atcccagaat aacaggcaaa gccaaaacca agttgctgca
     1261 cggcataacc gccttgcagc acagtgaggt tgtcagcgaa aaaccagaca agcgcctgcc
     1321 caattacaaa gtaaaggaaa agatttacct tgagcggcag gtatgcctgc ctttgaaccg
     1381 cgcccggcgc cccatggttt ttcgcggcgc tgcgcaatga agcgcggaac accaaaacca
     1441 tgagcagagc gtgcaccacc tgcatgaggc tcatcacgag cggaaatccc tgccaggcaa
     1501 aaagaaatcc caggccgccc ggcagggtca gcagcgcaaa gagcgcgagc ggcaggaaat
     1561 tccggatttt catccccagc tgacgtaacg ctcttcaaca acttcaccgc aggtggaaca
     1621 cgtcggttcc tgccgtgaga ctttgccctc gaggcaatat gggcagacgc tatcgtccat
     1681 gagaattttt acctggttgc cgagttcata gccgcagtcg gggcaggtgg gattatggtg
     1741 tttgtcttcc cattcgcccc agcagtgcgg gcaactcgaa tgctggaaca ccctgatctc
     1801 gccgtcgcag agatagcgca cggctgcatc gctgttattg aagtccatcg tctcaccgtc
     1861 ggcaagccag gcgcggctgg tttcactgac gatgatttcg cgctcgcgtt ctgaatcaga
     1921 aaggcgcagc tgcagcgaaa gcgtcaccgc ctggtcttca ttgcccgtcg ccgccaatgc
     1981 cgtcaggtga ggttccagat cggcgaatgc cccctcctga actaattgca gaaaccgttc
     2041 ttcaatgagt atagcgagct cgtcggcttt ggcgtagttg atatcgggag acataggtta
     2101 aagacgcagg ggtcaagtgt gtgtaaatga cttggtagtc cagacccgaa atcaaatatt
     2161 gctattccgc acctgtttaa ttaatttcca aaccagtatg gcacagacaa tacatgaatt
     2221 cagcgccgcg actctcggcg gtaaagaaaa gagcctcaca gaatacaaag gtaaggtagt
     2281 acttattgtg aacaccgcga gtaagtgcgg cttcaccccg cagtacgcgg gccttgaggc
     2341 gatctacgaa aagtacaaag accaggggtt tgtgattctc ggcttcccct gcaaccaatt
     2401 tggcgcgcag gagccgggtt cagagagcga aatcgagcaa ttctgccagg tcaactacgg
     2461 ggtcaaattt ccgatgttcg gcaagatcga cgtgaacggc agcaacgcgc acccgctgta
     2521 taaatttctc acatctgaaa agccgggcat tctgggcctt gaagcgatca agtggaactt
     2581 cacgaaattt ctcattgata aagatggtaa ccccgtgaag cgttatgctc ccaacaccaa
     2641 gcctgaagat atcgccggcg atatcgaaaa gctgctgaag gcatgacgcc gcgccactgg
     2701 ctgttcagcg tgcgcgacgg ggttgcaacc ctgtcgctca accggccaga atacggcaac
     2761 aacatcaatc tcgagtgcct cgaagagctg cggcagctca gcgagcagat tgaacacaac
     2821 ccggcagtgc gtgttgtcgt tctgcgcagc gagggcaaac acagctcgat cggcatggat
     2881 gtcagcgtga tcgggcaaat ggccggccag cagtatgaac cctatgccga acacctcggc
     2941 gcggggcaaa aatgtatcga tgccttcgag gcgatttcgc gcccgatcat cgcagagatc
     3001 aagggcttct gcatcggcgc tggggtgatt ctcgcggcgt gtgctgactt tcgtatcagc
     3061 accgagcgcg ccgtttttgc gctgcccgaa gtgaagcgca gcattggcgt catcatgggc
     3121 ctgcaccgcg tgacgcgcct ggtgggagtt tcctcggtga aacgcatggc aatgctgggc
     3181 gaaaattttt ccgcgcggca gatgctcgaa tggggttttc tcaccgaaat tgttgcagaa
     3241 gaaaaactcg cggcggccag tgcaaacctt gtgaaaaaga ttctcgcatt gccgccgctc
     3301 gcggtaacac tcaataagcg catagccgat ttttcatacg ccgcgttttt gcagcagtcg
     3361 cagcagtttg aactgcgcga gcaatttcaa ctcaaccaaa cggcagattt tcgcgaagcg
     3421 attgaatcgt ttcaggaaaa gcgacccccg tcttacctcg gaaaatgatg cgcctggcaa
     3481 gaattttgac ctgctgcggc ctgatggcac ttgccgcatg cgctcccccg aaagcgaaaa
     3541 agccagccac tgcccgccac attctgtacg ctctgcctga ccagctgccc gaaaagtacg
     3601 attaccgtgt gcagggaatg caggcaaccg gtgttgcatg gcttgccgcc agcttcaagg
     3661 gcgacggcag cctgcggctg caacaccgcg atgcagtcga actgctgggt cgccagcgcg
     3721 ccgacctcga acagcattta ctcatcacag aggcttcagt cgatgaaggc aaccggttat
     3781 tgaacacgcg cgctgccaca aagcgagcgg cgaataatct gcgcgaggct gtcgtgggca
     3841 tgcctcacat ccgtgcggtg cacctggatt ttgagtacaa tggcccgggc ctcgccgaag
     3901 cgtacgttga atttgtccac actttgcaca ctgcgctgcc agagccagtg agcctttatg
     3961 tcacggtttt ccccccggtc ggtatgccgc agaagtggag cgccttcttt cttcccgaac
     4021 aattggcgca cttcgccgac ggcattgtgt tcatgctgta tgatctgcac cgcaacggta
     4081 cagccgcagg ttgtgtgagt cccatttcgt ggctcgatgc caatgtcgca gaattttcaa
     4141 agctcccacc tgaaaagatt tggttgggag cgccacttta cggttaccaa tttacgggaa
     4201 aaaaggcaac cgcgttgtcg aagcgcgggt ttgaaaagat cgtggcgcaa gaaaccgaag
     4261 cagatggttg ccgtatgaaa aagaccggcg ccagcgagac ttactacccc gcaccatctc
     4321 tctatgcgca ttacgaaaag ctcgtagcga aacacggctt tgcgggtatt accttctggc
     4381 gggcggggtt agagaaatga aagcgatgac aaaacttgcc atcatcggcg acgcgcacat
     4441 gcagtttgat gaagaagacg tgcgcttctt caatgcatca gattatgatg cgatactctt
     4501 tgtcggcgat cttgcatcca aaaatcctga cagcatgttc aggctgctgc cagttatcaa
     4561 cgggctgacc aaaccagcgt tcctgattcc gggcaaccac gatacgaccg gtgtgcgggc
     4621 aattatcggc gaagtcacgc agaaccagtt cctgatcaac ttcggagcgt cttcacaacc
     4681 gggccgcatg cagcggctga aggccgaact cacgggagca acgctgtgcg gctattcgct
     4741 gcatacgctg ccgggcaatt tgcagatggt ggccgcacgg cctttttcaa tgggtagcac
     4801 caacaggaca atcaacttca ggccctttct cgagaaagaa tatggcgttt cgaccgttga
     4861 acagagccgc gacaagatca agagcctcat tgaccaggta aactccccct acatcattct
     4921 tgcgcaccac ggcccatttg gcctgggcag caaggcgacc gacatgtggg gtgcggattt
     4981 tctgccgcaa gaaaccgact tcggcgatgc tgatcttgcc gaagctgtta cctatgcgca
     5041 atcttcaggc aaggcaccgc tcgccgtgat tgccggacac atgcactacc cgacaaaaca
     5101 cggcaagaag ccgaagcagt ggtgggccat gaaagacggg gtcttgtaca tcaatgcggc
     5161 gcgctggcca cgtatttttc ggcatgaagg caggctgatg caccaccatg tgcagcttgt
     5221 cattgatgag cagggtaaat ctactgtatg tgcatgctat acctgtgaag ggaaaatttt
     5281 gccggtctac gacgatcggg cactcgtacc tgaagcattg tagcagttta cgcgagcaac
     5341 ccaccatagc tattggaccc tgccggactc atcggcgaat gcatattatg aaaaagaaac
     5401 tgggcctttt ggcaatgatg accgtactgg tgggtattct cacatggtat atttttccgt
     5461 cggttttttt tgaaccgctc gtgaacctga accgcaaact ttcgagactc accgagaaga
     5521 cgacgcagat atccggtcac accgtacatt acctcgaggg cggccagggt gagacagtcg
     5581 ttctgctgca tggaattttt gccgaaaaag accactgggt cgaattcgcc cgcgatctta
     5641 ctccgcacta ccgggtcgtg attcccgatc tgcccgcatt cggcgaaagc agccgcaatg
     5701 aaagcgaggt atacgactat gcaaaccagg tgagtcgttt agccgagctt ctgcagaagc
     5761 tgggtatcag caaggcacac cttgccggta gctcaatggg tggaaccatt gcggcattgt
     5821 tcgctgtcga aaatccacag gcggtgacga gcctggcatt catcggtgcc ccgcacggca
     5881 ttcgcagcac gaagcaaagc gacatggatc gtatgattgc cgaaggtcgc gctgcactcg
     5941 ttgcctcttc gcccgctgaa ttcgatgcga tgatgcagtt gctgtttgcg aaacagccgt
     6001 ttctgccata ccctattctg catgaagcga aatcgacagc tctcaaaaat gcattctcta
     6061 accggcgcat ctggaaccag cagctcaaag accgctatct gctcgatggc attgtcggca
     6121 aagtaagtgc tccgactggt attttatggg gagccaaaga tcgtatcttt gacttttcag
     6181 gcatgaccac gctcagggag aaactgcctg cagccaaagc cgaactggca actgaggcag
     6241 gacatctgcc gatgatggaa gccccgggcc agacagcagc ctggtataaa tcgtttctcg
     6301 cctcacagaa gaagtagccg taagatcggc atagaggtac agcatgagaa ctgaaagact
     6361 ggcagcttac tttaccgaaa aatcaggtac gcctgcgaaa attaccgaat ttcgccagct
     6421 gtcgggcggc gcctgccagg ataactacct ggcgcgaata gaaagtgcgg gtggctcaga
     6481 agatcttgtc ctgcgcaccg acaaaggagg cgcgctgctg ggctcgctca cgcggcctca
     6541 ggaataccag gtcatcgaac gcgcggtggc agcgggtgtg cagacccccg ctgtaaaatg
     6601 gctcagtgaa gacacggcga ttgttgggca cccgttctac ctcatgcaaa aaattgaggg
     6661 tacggcgaac gcgcgcgaaa ttctcaagag caggcaaatc gatttcaaac gccttgcggc
     6721 ggagctcgca atgaaccttg ccaaaataca cagcattgaa tattcaccgg cgattctgcc
     6781 gtttctcgat cgcggccttc ggcccgatac gaacgatgtc gcgctgacgg cagtcgccga
     6841 ttgccgccgg ctgctcgatg agttgccggg agggtaccct gcgattgaac tctgcctgaa
     6901 ctgggccgag gccaatgcgc cggtgaccga caaggtcgtg ctagcgcaca gcgacttccg
     6961 ctcgggcaat tttatgatcg gtgagtccgg gcccacaggg cccggactca ccggcatttt
     7021 agattgggaa ttcgcgcact ggagcgatcg ccacgaagac atcggttaca tacgcatgcg
     7081 cgactggcgc tttggcaaag taaaaaatcc catcggcggg tttgctgcgc ctgaagattt
     7141 ttaccctcac tatgaagcag aggcgaaagt gaagctcgat gaaagtaaga tccgcttttg
     7201 ggagatcatg ggcaacctgc gctgggcgat cggtgcagcc caacagggcg agcgtcacct
     7261 gtcaggtaaa gaccgcggca ttgaatttgc ctcgatcggc cgccgggtgg cagaaatgga
     7321 actcgaggcc atgcgcctca tcgagcaagc gtaggaacca actcatgcaa taccgtccgc
     7381 aaaataacga cctgatcgac gcgatacagg aattcctgct caaagaagtt ctgccgttcg
     7441 tcaaagagaa cgatgcactg gcctacaaga cacttgtgtc atggaacatg ctcggcgtag
     7501 tctcgcgcga actgaaaaat ggccggcccc ttttggaggc cgacgcgaca ctgctggcag
     7561 aactcctggg cgagaaggca gaagtacaga caactggaga ccgcgagctg tggcaacagg
     7621 cacaggcgct ggcgaaaaaa gcagcacaga aggtacgcgc tgaacaaacc atgcccggca
     7681 gcgctgagtg gcacgcagtg aagaagctgc ttaccgacaa cctgcagatc tcaaacccgc
     7741 gatatggagt ggaatgactg agccacggat ggcgcgccat ggccgggata aactgcaatg
     7801 actgaactgt atctcgtccg ccatggccag gccgacagcg ctggcgataa ctacgaccag
     7861 ctgacggaaa ccggccacga gcaggcacgc cttgtgggcg aatggctggc cgagcggggg
     7921 tatactttca cgcgtgcatt gcacgggggc ctacgccggc aacaagagac gctggaggca
     7981 attgccgcag cttatgcacg gcacgggcag gatctgcctg cggcggaatt gcacccggcg
     8041 tttgccgagt tcgacctgcg cgtctgggca attatcgccg gcaaactgcg gcacgggcat
     8101 gctgaatttt ccgagctgct gaaacaatgg aaccgcgccc ggcatgagaa ttctcccgac
     8161 aaaggtgaca tcttcaagca gctgacgggt atcattctgc gcgaatgggt ggcgatgggt
     8221 gagcatttta ccgaagcaga aagttttccc gcgtttcggc ggcgcgtgct cggcgcactg
     8281 gaactgcagg ccacgccggc gaccgcttca ggcaaggcgc agcggatcct ggctgtaact
     8341 tcgggcggcc cgattgcgtt gctgacgggc ggggctctcg ggctctcgca gccaaaaact
     8401 ctcggactca tgcggcgtat ctacaacacg tcgctgcacg cgctggtcta cgacaagagc
     8461 caatgggagc tacagacttt caacaacgta ccgcacctgc cagcggcaga tcgtacgctg
     8521 gtttaataac tacgtgattg tggccaagcc acaatcacgt agtttaaatc acggctgcga
     8581 gtagttttct agcaggatgt aggccgtacc gaggtatggc acttcagtcc agattttgac
     8641 cgggacaaaa ctttcatcgg cagtgatcca catatcgatc tgcgactgcg tattaaagaa
     8701 gatattgccc tgaggttgtt tgcccttgcc ttcgtctgcc agcgccgact tcacatggat
     8761 aatttcacga gtgccaaaga aaggtacatc ggtacgcttc tcgcgcccga gaaccttcag
     8821 taccagcgta cgccgtgaac ctgcgggcaa gacaggtacc cgcaggacct cccccacttt
     8881 gggcactttg ccggtgctgc ggacatagta gaatgccccg agcaggtcct ggtagccgcc
     8941 gctcgtgctg acatcgtacg tttcgttaga ctgagacttt accggttcgc tgtgcgggta
     9001 actgagctcg tgaaagcgtt tttcattttt ttcgacgcgg acattacgcc cgccgaatcg
     9061 aaattttcgc cgctggtacg tatcgccctg gtaggcttct tcgtaggcga tgaccggcga
     9121 acggctggca aaattccatt tcgcggcgaa gaccccctgc acgtaataga ttccgtgcat
     9181 gaccggaaga gaattcacct tggcgttgat cgttaagaag ttattgcctt cgatgcggct
     9241 gcgcatcgtc acgtcgcccg cacggatacc cttccattca atgcggaaat tcatcgtttc
     9301 ggtgtaaaat ggctgccttg cctgcgctga aatacctgcg gtcagtgcta ctaccagcag
     9361 accagcccgg ctcaggaaat tgaacatggt tttgcctgtt aactccggtg gtcgggtcaa
     9421 ggagaattct tgacccgatg gcctgaaagg cgaggcaagc caatgtctgc acaaaaaaca
     9481 gtcctcatca ccggggccac gcgcggtatc ggtaagggta tagcgatcgc attcgccgat
     9541 gccggctaca tcacctatgg aaccggcaga aatctttctg atacgtcttg ggcgacggag
     9601 aagaaaatca ttctgaaaca ggccgatgtc tccgaccagg cagcgatggc agcaatcatg
     9661 accgaaataa aagagaagca cggcaggctc gactgcctca tcaacaatgc cggggttgcc
     9721 tcgaacacgc cggcatcttc gctcggcgaa gatgaaatcg caaaaatcat cgataccaat
     9781 tttaaggcag tcttccagtg ctgccagagc tactacaaga tgcagagaaa ggctggcggc
     9841 aacatcatca acatctcgag cgtgctcggc atcgtgggta cctccctcgc ttcggtatat
     9901 tcgggaacca agggagctgt gatttcgatg accaaggcac tcgcgattga atgggcgaac
     9961 tcaggttttc gcgtcaacgc gatctgccct ggctttatcg atactgacat gacagaaatg
    10021 atcaagaaac gcgaagcggt aatgcagaag atgctcgagt acattccgct caagcggctc
    10081 ggcacgcccg aagacatcgc cgcgcccgca ctgatgctgg cgtcagacgg agctgcttat
    10141 attacgggtc agatcatcgt ggtcgatggc gggttgaccg cgatgtaaaa cgtttgtgca
    10201 accggcattg ccggttgcac aagttagcta gatagattga aggtatttcc agagcgcctt
    10261 catctcagta tcggtcagct taccgagact ctgccagggc atcggaaatt tgatcatact
    10321 accatcgggg cgttttcctg tgcgcagcgc ggcgataaac tgtgcctcat tccacttgga
    10381 gagtgccttt gcagtgatcg gctgtgcggg tggccattcg ggtggtgctc cctgtatcgg
    10441 gccgccggca aaattgtcgc ggtggcaacc cgtgcaggtc gcggcgacgt acttaccata
    10501 ttcgacgctt acagtgggat taatttttgt tacaggtttt gcattgtgat cgattctttc
    10561 ggccgaaaca aacacaggta tttccccgat tagaaaaaga aaacgaccga ggggcccagc
    10621 tttgggcggc agaaactcac gatcgactgc tggcacgctg cgcaggtacg agatcagctt
    10681 gcccgtatct tcgtccgtca tacccgtata gtctgttgca ggcataaaaa ccagcgcccg
    10741 gccgtttttg ctgacaccgt ggcgaattgc acgcgccatt tcggcgtcgc tgcgttcttt
    10801 accaatgcca cctttaccgg cggtcaggtt agccccagcc atggtgccaa ttgcgggatc
    10861 gttgatgaat accctgccgg catggtcgtc gccatggcag tcggcgcacc cgcgtgaaat
    10921 gtagagccgc ttaccttcgg caatatctgc agcagcttta aaggcaggaa caggtgtagc
    10981 agccacgtca tattttttat tcatgcgagc cgaactgagc acaaaaatga cagtaacccc
    11041 tgcggcaatg agtatggtca gagagagcaa tccccagccg agaatttttc cgagtatctt
    11101 catttgacct ccgaactttt tgagtcggcc tgcttgcaga gaatgcggcg cgatgtaaac
    11161 agcgattctg ccttttgggc tggtcttgaa cttagctgag aaatcgcctg atgaggcgaa
    11221 agaatcggag ctttttgagt ggtaggttca gccagccatt ggtgatgcag aaatatccgc
    11281 tgtgcggcgg ggtatggtgt atcgcgtggc cttcgggtgt aagaatcagg tggagcttct
    11341 gcagcacacg tgggagtttc gggggcatgg gcatatgcgc ccacttgtgt atctggttcg
    11401 tcaaaaatat cgctcccaaa aagaagaaga tccagagacg cagcagcggg ttcgattctg
    11461 cctcagttgc cagcagcaca atgcacagca ccggcagcga tatgatgctg ttggcgccgt
    11521 tcgtttcaag aaagccgtgc cgcgtaatcg cgagcgg
//
LOCUS       DAOQJF010000066        11437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2858_length_11437_cov_4.760675, whole genome shotgun sequence.
ACCESSION   DAOQJF010000066 DAOQJF010000000
VERSION     DAOQJF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2858_length_11437_cov_4.760675"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLF85_05230"
     CDS             <1..1074
                     /locus_tag="PLF85_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE19096.1"
                     /translation="FQYEPGSTFKIFTMAALLRENMLDDRTEYVCPGYYEYKGRKINC
                     PRQHGRQNFTEVMKNSCNYGVIAASWQLPVLKLYENLKAYGIGTQTEIALPGEAKGYL
                     ASPKDWDYYLKMSIPIGHGVSVTPLQLAIAANAIGNDGKFMPPMIIDKVTDAQGQVIE
                     RFAPQVKYRVASAENAQTIRRTLREVVKSGTGAGADLGLKEFDVCGKTGTSIKSSNKG
                     YDAKKYQASFVGFFPCDKPEITIMLMFDEPRGEYHQGATVAAPAFRRVLREIIPAIHV
                     GDVSEVRPLVTPAYRSAGQDKSVMPDFRGFSKKELLFQVLSKFPGDHQVKGAGYVTAQ
                     TPAPGTKINPPYNFSFTLGFPDE"
     gene            1279..3369
                     /locus_tag="PLF85_05235"
     CDS             1279..3369
                     /locus_tag="PLF85_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNE19097.1"
                     /translation="MEHLLKQLLQYTAALCFFVGAFSLAAQDSAPKDAPKQVDQMAAP
                     VKAETPPAPAEKKPEAAPAPAPVAQPAAQPVRKQPVIPPTKKNEAGSESSPTGNPPDS
                     QLEKLATESDKDTESEAPVKDKPGKKGKTADEADKLAKDDKKKGKKDKGKDSFFRPDF
                     RDQEIQDILKIFSKIIGKNIIADEKVKGKITVISPYKIPSSLAYPYLYSMLAIKGFGI
                     VEENENLIRVVAIKDALAGSPLIFLGREEIKDTEIKSDLPVTQILPVYGSKPSRLSAI
                     LKRLTSANTDLVDYDDISMLIITGSLFEVNRLIRISNLIDYQGPAPECDEKKDPDCGR
                     VRVDGSVHVYRLENMQAENIEATLKKVQLPVEPLPSAPAASGQASAPAVTQANVQKKP
                     IDVIAHKETNSIIYVGTNDEFELVKSLIKRIDLQRQQVFLEVLIVEVGVDKNNLFGID
                     WIFGRGGSAQFNSGVALKSGLIDTTSGSFKNGAINQNSGFSLTFLNDSVANIIGILNA
                     QVGKSNFVVVSAPQVLTLDNQEAEINVGQDVPVRVNSFTNVTGTQSNSFEYRPVGIKL
                     KFTPQVNKNRMVTINLFQEVKSLIPSSDPTANPTIAKKDIKTFVRVQDGQTIVIGGLV
                     SNDRTATISKVPILGELPLVGFIFRRTETITKRRNLMVFITPHILTNRAIADKVTEDL
                     HQDQIKEYKRNSID"
     gene            3374..5068
                     /gene="gspE"
                     /locus_tag="PLF85_05240"
     CDS             3374..5068
                     /gene="gspE"
                     /locus_tag="PLF85_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNE19098.1"
                     /translation="MRKFGDILVEEGLIDAGELKKALTLQRKSAYKLGQILIKKGLIS
                     EEDALKALSKQYEMSFADKLEPLNIEQLIEKVPLKFVQKYRVVPYYIQEKEIRIAMTD
                     PSILHPLDEFRLLFIGYEVTPVLTRESEILRIIHNFYEAKSRAEDTDLEMGDGLEILD
                     DIQDLHDSAELANQAPIIKLVNMILSNAVNEKASDIHFEPMEKEFLIRFRIDGVLHKM
                     MSPPKSIQSGVISRVKIMANLNIAENRLPQDGRIRLRFSGNDIDIRVSSLPTQHGERL
                     VLRLLNRTAAKYDLATIGFEKRTYEQFVETINYPNGIVLITGPTGSGKTTTLYAALTR
                     LNSPERNIITVEDPIEYQIDGIGQVQARSKIGFTFAEGLRSILRQDPDVIMVGEIRDE
                     ETARIAIQASLTGHLVFSTLHTNDAPSAVTRLLDMGIEPFLVTSTCRGIMAQRLVRKL
                     CVHCRKPKTVNAKDLQSIGLLYHNPKKGEVPTGKIRIYEPSGCKECMNTGYRGRMGVY
                     EFMPITAKLREMIITRASLDDLKDQAIHEGLITLRNAALRQVVAGVTSLDEAMRIT"
     gene            5105..6325
                     /locus_tag="PLF85_05245"
     CDS             5105..6325
                     /locus_tag="PLF85_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNE19099.1"
                     /translation="MAHYQYTAFSPKGQKVTGVIEAISPQAATQKLKSQNLYVREIRA
                     DSAKRDRELFPFLSNFLYRIKRKEAGIFARQLGTLLGAGIPLNEALSDISDQTENPHL
                     RKIIQEMKQQVIEGRSLSEAINQHTNVFPPVYESMVRVGEATGNYEPTLKRLADLEER
                     NEELKARAITAMIYPMFMLLISVGVVFFLMTSVVPQLSDLFSGFKAELPLITRVVIGL
                     SKLFSSFWYVMILAAFGGIYAFVRYRSTKEGRQKTDRMIMRIPFFGGLLRRIQVGLFC
                     RNLGILVESQVPLLDALKIVSGTVTNSIFVDELTAAIQQIKEGSSLRESLKDSVILPQ
                     MARGMIAAGESTDRLAELLIKAAEIMESEVDSAVRRMTQSLEPIMIVFLGGIVAAIMA
                     AVMLPMYKMTEYIK"
     gene            6335..7525
                     /locus_tag="PLF85_05250"
     CDS             6335..7525
                     /locus_tag="PLF85_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE19100.1"
                     /translation="MLLRLLEILSGARRRLVRLRRIFLRKVIPPKILVIRNDGLGDFI
                     LTLPIISALREQIPHARIHVLAARSLHSMAAFLPEIDGWVTDPGCLLKRHRRGKSALQ
                     LDTEYQNLVREVSAYRFDLAVFCYAEKHSARLAKDAGIPYRLGPLRRSFFTKFNLWYY
                     IPRRKTGQSEFQLNLKILRSLRVENIFRLPRAELPALSQKVETGRYIVMHPYKRSGTA
                     LVWPMENFQALARYYSAEKRKVIVIGDRDDAEVLHAYFGAIPQVKIQTELNLVQTAAL
                     LKGAEHFFGNSSGPLHLAALVGTPHTGFYPQNKIASPRRWRTLPNERTPRLSEHLLAT
                     DFPVDCTSCKLEKCDYYPCTARIETAAAIRSVSAWRKTAPTRKARSAAKPKRRRPARK
                     RKKL"
     gene            7637..8896
                     /locus_tag="PLF85_05255"
     CDS             7637..8896
                     /locus_tag="PLF85_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19101.1"
                     /translation="MDHFRAYSLSIASHALEELSEFPDSRAFYTDARQLPAPAPEIRS
                     LLYMATCNRIELYVEAEPGVETTLVEEKLAAVSPVFALMLAKAPQVYAGGKMLEHMIA
                     VASGLKSLALGETQIAGQLKRDMAYAQETGWLGAGMMTVIRKALETQKKIRTSTGISE
                     NSYSLMSLVEDTIAQRSLHPLSGNLILVGASEMSAKVARFALRRNVGQFLLVRKDLSR
                     PMNADLNTLIQTYPEKFLQVTLENLAEERKNFSATAIILASSSPHPLFSAQDIQLLQS
                     NYVLHADSAIVDLSLPANVHPDVGAVLGDRLISLDSLKNVSEEARADRLSSAAQAEPI
                     IRRAVYQFWLDTLYRDNPELVQDYLDAKSQQTESEWQRLAQEASLSEKQKRILYDFLK
                     KEQRRALSSHREMILDLMAGAGKVRQA"
     gene            8899..10605
                     /locus_tag="PLF85_05260"
     CDS             8899..10605
                     /locus_tag="PLF85_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19102.1"
                     /translation="MPAKEITLASRDSLLALTQTIEAAMRLEAAGFAPRIVSMKTAGD
                     IKLNAPLYSVAQQSGNKEGRAFFTRELDDALLSGRADAAVHSFKDLPTEKVPGIGEPY
                     FFCEEHGSDVLLLGEKTVMSTDGAGLVIGTSSLRRIHQLQLVLPAARTVTLRGNVITR
                     LEKLLAGENEINAILIAAAGLRRLTAFAEVPENRYAHLVVPAALEHIRRELARLAASE
                     AARARAIELPERYFPTAPGQGVLALQLSAEAEARYSGAVAAVFAEHQQIARRVMQERR
                     IMTELMTGCHAPLGVSALRGDEHRIVACYSRKSTTEPVSFSDSVWFERELGADSTAIA
                     QELKLPREQIYWWGFKAPPQNSGLPLQFVPAVEQKPLEVQWSGALPEAVFVASPRAAE
                     WVSAQKDLLNLPFYTAGAETGHVLKSLIPAAQITPVAGKGFAQALKVFSNKQVQLLWV
                     GSADGEKRARATAQEYPAARFLPVYTNLPLRPKLPAPVAGALHVLTSAMAAEAFVRWA
                     AEAGNIDPQICCFGESAVEIVKATGMPVYHASEANDFSALVRELKGDTGLLRERWQLK
                     EF"
     gene            10609..>11437
                     /locus_tag="PLF85_05265"
     CDS             10609..>11437
                     /locus_tag="PLF85_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE19103.1"
                     /translation="MERKKSDKLWQRALKVFPGGVNSPVRAFRSVGGNPLFIASGKGS
                     YVRDADGNRYLDFLSSWGPLILGHAPRPVVRATRKALRHGSTFGATTEAEVKLGEFIL
                     QNIPFLERVRFVSSGTEAVMTALRLARGTTRRQMIVKFEGCYHGHNDALLARAGSGLL
                     TLSGNIAEASSPGIPDEIVQNTLVLPLDDSAAFEQALEKHGEKIACVIIEPVPANAGL
                     LLQRRAFIEHVLTSARQKGILVIFDEVISGFRLSFAGYAGRENLKPDIITYGKIIGGG
                     "
BASE COUNT         2722 a         3319 c         3065 g         2331 t
ORIGIN      
        1 tttcaatacg aacccggttc gacatttaag atcttcacca tggctgcact gctgcgcgag
       61 aacatgctcg atgaccgcac cgagtatgtc tgcccgggtt attacgagta caagggccgc
      121 aagatcaact gcccgcgcca gcatggcagg cagaatttca ccgaggtcat gaagaattcg
      181 tgcaactacg gggtgatcgc cgcttcgtgg cagctgccgg tgctcaagct ctacgaaaac
      241 ctgaaagcct atggtatcgg cacgcagacc gaaattgcgc tgccgggcga agccaaaggt
      301 tacctcgcaa gccccaaaga ctgggattac tacctgaaga tgtcgatacc gattggccac
      361 ggtgtgagcg tcacaccgct gcagctcgcc attgccgcaa acgcgatcgg caacgacggc
      421 aaattcatgc cgccgatgat catcgacaag gttacagacg cgcagggcca ggtgatcgag
      481 cgctttgcgc cccaggtcaa gtaccgggtc gcaagcgcag agaacgccca gacgatcagg
      541 cgcaccctgc gtgaagtggt aaaaagtggt acgggcgccg gcgccgacct cggcctcaaa
      601 gaattcgacg tctgcggcaa aaccggtaca tctatcaaat cgagcaacaa gggttacgat
      661 gccaagaagt accaggcatc gttcgtgggc tttttcccgt gtgataaacc tgaaatcacc
      721 attatgctga tgttcgacga gccgcgcggc gaataccacc agggcgccac cgtcgcggcc
      781 cctgcattcc gccgcgtact gcgagaaatc attccggcca tccacgtggg tgacgtgagc
      841 gaggtgcgcc cgctggttac ccccgcatac cgctcggcag ggcaggacaa gtcagtgatg
      901 cctgatttcc gcgggttcag caaaaaagag ctgctcttcc aggtgctttc caagtttccg
      961 ggtgaccacc aggtgaaggg tgcgggttat gtcactgcgc agacgccggc cccgggcaca
     1021 aagataaatc ccccttacaa ttttagtttt acccttggct ttcccgatga gtaaagagag
     1081 gaaacagcca gtggcgcctg ccctggtatt tttgtctcga aaaacagcaa tttgccgtct
     1141 caccaggtaa acagtgaaaa cacccctgcg tattcccttc tttccgcttg ccggcaaatc
     1201 gccatatcgg cctctggcgg cagccgatag gttgcctgcc gtgttttacc ctgatttttc
     1261 cccgtcattc tgccgcccat ggagcacctt ctgaaacagc tgctgcaata tactgcggcc
     1321 ctctgctttt ttgtgggagc tttctctctg gctgcacagg actctgcgcc caaagacgcg
     1381 ccgaagcagg tcgaccagat ggcggctccc gtaaaggcag aaactccccc tgccccggca
     1441 gaaaaaaagc ctgaagctgc gccagctcca gcaccggttg cacaacccgc agcccaacca
     1501 gtcagaaaac agcctgttat acctcccaca aagaaaaacg aagccggcag cgaatcttca
     1561 cccaccggca acccgccaga tagccagctg gaaaaactgg caacggagag cgataaagac
     1621 accgagagtg aagcgcccgt aaaagacaag ccgggtaaaa aaggcaaaac cgccgacgaa
     1681 gctgacaagc tggcgaaaga cgataaaaag aaaggaaaaa aagataaggg caaagacagc
     1741 ttctttcgtc ccgactttcg cgaccaggaa atccaggata ttctcaagat tttttctaaa
     1801 attatcggca agaacattat cgccgacgaa aaggtgaaag gcaagatcac tgttatttca
     1861 ccctataaaa tacccagctc gctggcgtac ccctacctct actcaatgct cgctatcaag
     1921 ggctttggta ttgtcgaaga gaatgagaac ctgatacgcg tggtcgcgat caaagacgcg
     1981 ctcgctggca gcccgttgat ttttctcggc cgcgaagaaa tcaaagatac ggaaatcaag
     2041 agcgacctac cggtgacgca aattctcccg gtctacggca gcaagccttc acggctttcg
     2101 gcaattttaa aacgcctgac cagcgccaac accgatctcg tcgactacga cgacatcagc
     2161 atgctgatca tcaccggtag cctgttcgag gtcaaccgcc tgatacgcat cagcaacctg
     2221 atcgattacc agggcccggc gcccgaatgc gacgagaaaa aagatcctga ctgcggccgc
     2281 gtgcgcgtag acggcagcgt acacgtctac cgtctcgaga acatgcaggc cgagaacatc
     2341 gaagccaccc tcaaaaaggt tcagctgccg gtagagccac tgccttctgc acctgctgct
     2401 tctggccagg caagcgcgcc ggcagtaaca caggcgaacg tgcagaagaa acccatcgac
     2461 gtcatcgcgc acaaagaaac aaacagtatt atctacgtgg gcacgaatga tgagtttgaa
     2521 ctggtaaaga gcctgatcaa gcgcatcgac ctgcagcgcc agcaggtgtt tcttgaagta
     2581 ctcatcgttg aggtgggtgt tgataagaac aacctgttcg gtatcgactg gatattcggc
     2641 cggggcggca gcgcgcagtt taacagcggt gtggcactca agtcaggtct catcgatact
     2701 acatcaggca gtttcaagaa cggcgcgatt aaccagaaca gcggtttctc cctcacattc
     2761 ctcaatgatt ccgttgcaaa catcattggt atactgaacg cgcaggtcgg caaatcgaac
     2821 ttcgtcgtcg tctcggcccc gcaggtactg acgctcgaca accaggaagc agagatcaac
     2881 gttggccagg acgtaccggt gcgcgtgaac agcttcacca acgttaccgg tacgcagtcg
     2941 aacagcttcg agtaccgccc ggtcggtatc aaacttaaat ttacgccgca ggtgaacaag
     3001 aaccgtatgg tgaccatcaa cctgttccag gaagtgaaat cgctgatccc gtcgagcgac
     3061 ccgactgcca acccaacgat tgccaagaaa gacatcaaga ccttcgtgcg cgtgcaggac
     3121 gggcagacga tcgtgattgg cgggctcgtg agcaacgacc gcacggcgac aatttcaaag
     3181 gtccccattt tgggcgaatt gccactcgtg ggttttatct tccgccgcac ggaaaccatc
     3241 accaagcggc gcaacctcat ggtctttatc actccgcata ttctgactaa ccgcgctatt
     3301 gccgataaag tcacagaaga tctgcaccag gaccagatca aagaatacaa gcgcaacagt
     3361 atcgactaaa cgcatgcgga aattcggcga tatacttgtc gaagaagggc tgatcgacgc
     3421 cggcgagctg aagaaggccc tgacgctgca gcgcaagtct gcctacaaac tcgggcagat
     3481 actcattaag aaaggtctca tctctgaaga ggacgcgctc aaggcgctct cgaagcagta
     3541 cgagatgtcg ttcgccgaca agctcgaacc gctgaatatc gagcagctga tcgaaaaagt
     3601 accgctcaag ttcgtgcaga aataccgggt ggtcccctac tacattcagg aaaaagaaat
     3661 ccggatcgcg atgaccgacc catcgattct gcacccgctc gatgaatttc gcctgctgtt
     3721 cattggctac gaagtaacac ccgtactaac gcgtgaaagc gagatactgc gtatcatcca
     3781 taacttctac gaggccaaga gccgcgcaga agacaccgac ctcgaaatgg gcgacgggct
     3841 cgaaattctc gatgacatac aggacctgca cgactcggca gaacttgcca accaggcgcc
     3901 gatcatcaag ctcgtgaaca tgatcctgtc taacgcggtg aacgagaaag catcggatat
     3961 ccacttcgaa ccgatggaaa aagaattcct gatacggttc cgtatcgacg gtgttctgca
     4021 caagatgatg tccccgccga agtctataca gagcggcgtg atttcacgcg tcaagatcat
     4081 ggcgaacctc aatatcgccg agaaccgcct gccgcaggat ggtcgtattc gcctgcgctt
     4141 tagcggtaac gatatcgata tccgcgtgtc gtcgctgccg acacagcacg gcgaacgcct
     4201 ggtgttacgt ctgctgaacc gcaccgcggc aaagtatgac ctcgcaacga tcggcttcga
     4261 gaaacgcacg tacgaacaat ttgtcgaaac gatcaattat cccaacggta tcgtgctgat
     4321 tacgggcccc acgggttcgg gcaaaacgac gacgctttat gctgcgctca cgcgcctcaa
     4381 cagccccgag cgcaatatta ttactgtcga agacccgatc gaataccaga tcgacggcat
     4441 cggccaggtg caggcgcgct caaaaatcgg cttcaccttt gccgaaggtc tgcgctcgat
     4501 tctgcgccag gaccctgacg ttatcatggt gggtgagatt cgggacgaag aaactgcgcg
     4561 catcgctata caggcatcgc tcaccggtca ccttgtgttc tcgaccctgc acaccaacga
     4621 cgctccttcg gcagtgacgc gtctgctcga catgggtatt gagccgtttc tcgttacctc
     4681 aacctgccgc ggaatcatgg cgcagcgcct tgtccgaaaa ctctgcgtgc actgccgcaa
     4741 accgaagacg gtgaatgcca aagacctgca gtcgattggc ctgctctacc acaaccccaa
     4801 aaaaggcgaa gtaccgacgg gtaaaatacg catctacgaa cccagtggct gcaaagagtg
     4861 tatgaacaca ggttaccgcg gccgtatggg ggtctacgaa ttcatgccta tcaccgcgaa
     4921 actgcgcgag atgattatca cgcgcgcctc gctcgacgac ctgaaagacc aggcgattca
     4981 cgaaggcctt atcacgctgc gtaacgcggc gcttcgtcag gtggtcgcgg gtgtgacctc
     5041 gcttgacgaa gcgatgcgca ttacctgata gtagcctgtg gagccataag tcaggtaaat
     5101 tttcatggcg cattaccagt ataccgcatt cagtcccaaa ggccagaagg taaccggcgt
     5161 cattgaagcc atatcgccgc aggccgcgac gcagaaactc aaatcgcaga acctgtatgt
     5221 acgggaaatt cgcgccgatt cagcgaagcg cgaccgtgaa ctttttcctt ttttaagtaa
     5281 ctttctctat cgcatcaagc gcaaagaggc aggcatcttt gcccgccagc taggcaccct
     5341 gctcggcgca ggcataccgc tcaacgaagc gctgtcggat atcagcgacc agaccgagaa
     5401 tccgcacctg cgcaaaatca tccaggaaat gaagcagcag gtgatagaag gacgctcgct
     5461 gtctgaggcg atcaaccagc acacgaatgt ttttccgccg gtttacgaaa gtatggtgcg
     5521 cgtcggcgaa gctacgggta attacgaacc taccctcaag cgactcgccg accttgaaga
     5581 acgtaacgaa gaactcaaag cgcgcgccat tacggcgatg atctatccca tgttcatgct
     5641 gctgatttct gttggcgtgg tgttcttcct catgacgtcg gtggtgccgc agctatctga
     5701 cctgtttagt ggttttaagg cggagctacc cctgatcacg cgagttgtca tcggtctttc
     5761 caaacttttc tcttcgttct ggtacgtgat gattctcgcc gccttcggcg gcatctacgc
     5821 gttcgtccgc taccgcagca caaaagaagg ccggcaaaag accgacagga tgatcatgcg
     5881 catcccgttt ttcggcggcc tgctgcgccg catccaggta ggactcttct gccgcaacct
     5941 gggaatcctt gtggagagcc aggtaccatt gcttgatgcc ctcaagatcg tctcgggcac
     6001 ggtgacgaac tcgatcttcg ttgatgaact caccgcggcg attcaacaga ttaaagaggg
     6061 ttcttcgctg cgcgaatcgc tcaaggactc cgtaatactg ccgcagatgg cgcgcggtat
     6121 gattgcggct ggcgaaagta ccgaccggct ggcagagctt ctgatcaagg ccgccgaaat
     6181 tatggaatct gaggtggaca gcgccgtacg gcgcatgacg cagtcgcttg agccgatcat
     6241 gattgttttt ctcggcggta ttgtggcggc tatcatggca gcagtcatgc tgcccatgta
     6301 caagatgact gaatatatca agtaacgtaa gcagatgttg ctgagactgc tcgaaattct
     6361 ttccggcgcg cggcgacgcc tcgtcaggct gcgacggatt ttcctgcgca aggtaattcc
     6421 gccaaaaata ctcgttatcc gcaacgatgg tctcggcgac tttatcctga cgctgcccat
     6481 catttcagcc ctgcgcgaac agataccgca tgcacgcatc catgtgctgg cagcgcgcag
     6541 cctgcacagt atggcagcat tcttaccaga aattgacggc tgggtcacag acccgggttg
     6601 tttactcaaa cggcaccgca ggggaaaaag tgcgctgcag ctcgataccg agtaccagaa
     6661 tctcgtgcgc gaagtttcag cctatcgctt cgacctcgcc gtcttctgct atgcagaaaa
     6721 gcattctgcg aggctggcca aggatgcagg tataccctac cggctgggtc cgctgcggcg
     6781 gtcctttttt acgaaattta acctctggta ttacatacca cgacgcaaga ccggccagtc
     6841 ggagtttcag ctcaacctca agattctgcg ctcgcttcgc gtggagaata ttttccggct
     6901 tccgcgcgcc gagttacccg ccctttcaca gaaggttgaa accggtcgct atatcgtgat
     6961 gcacccatat aaacgttcgg gtaccgcact tgtctggccg atggaaaatt ttcaggcgct
     7021 cgcccgctac tattcagcag aaaagcgcaa ggtgattgtg atcggcgatc gggacgacgc
     7081 agaagtgtta cacgcctact ttggcgcgat tccccaggtg aagatacaga ccgaactcaa
     7141 cctggtacag actgccgcgc tactcaaggg tgcggagcat tttttcggca actcgtcggg
     7201 gccgctgcat ctcgctgcgc ttgtaggcac cccgcatacc ggtttttatc cgcagaacaa
     7261 gatagcctcg ccccggcgct ggcgtacgct acccaacgaa agaactcccc gcctgtcaga
     7321 gcacctgctg gcgaccgatt ttcctgtcga ttgtaccagc tgcaaactgg agaagtgcga
     7381 ctattacccg tgtaccgctc gtatcgagac tgcagcggcg ataaggtcgg tgagcgcctg
     7441 gcgcaaaacg gcacccacac gcaaggcacg cagcgccgct aagccgaaac gccgtcgtcc
     7501 cgcccgcaaa cgcaagaagc tttgagagat agctgttccg ctttttgaca tgttcctgac
     7561 aacaggcgcg caatgcgttt gtgccgcgtg gggcttttcg atatattcct gccagctcat
     7621 gaatggcgct ggcctaatgg accattttcg tgcatactcg ctgagtatcg ccagccacgc
     7681 gctcgaagag ctctctgaat ttcctgattc ccgggcgttt tataccgatg cacggcaact
     7741 gccggcgccc gcacccgaaa tccgcagcct gctctacatg gccacctgca accgcatcga
     7801 actgtatgtc gaggcagaac ccggggtaga gaccaccctg gtcgaggaaa aacttgctgc
     7861 agtctcacct gtctttgcgc tcatgctggc aaaagccccg caggtatacg cgggcggcaa
     7921 gatgctcgag cacatgattg cagtggccag cggcctgaaa agccttgcgc ttggtgaaac
     7981 gcagatagcg ggacaactga agcgtgacat ggcctacgcg caggaaaccg gctggctcgg
     8041 tgccggcatg atgaccgtca tacgcaaggc cctcgaaacg cagaaaaaga tcaggacctc
     8101 gacggggatt tcagagaatt catactcact gatgagcctg gtggaagaca ccatcgcgca
     8161 gcgcagcctg cacccgctgt ccggtaacct gatactggta ggcgcttcgg aaatgtctgc
     8221 caaggtcgcg cggtttgcac tgcgccgcaa tgtcggccag tttttgctgg tacgcaaaga
     8281 tctctcgcgg ccgatgaatg cagatctcaa taccctgata cagacctacc ctgaaaaatt
     8341 cctgcaggtc acgctggaaa acctggcaga agagagaaag aatttcagcg ccacggcaat
     8401 tatactggca tcttcttcgc cgcacccgct gttttctgcg caggatatcc agctactcca
     8461 gtcaaactac gtgctacacg cagactctgc tatcgtcgat ctctcgttgc cggcaaatgt
     8521 ccaccccgat gttggtgccg ttctcggtga ccgcctgatc agcctcgatt ccctcaagaa
     8581 tgtctcagaa gaggcgcgtg ccgatcgcct gtcttcggct gcgcaggcag aaccgattat
     8641 ccggcgggcc gtgtaccagt tctggctcga caccctttac cgtgataatc ccgaacttgt
     8701 gcaggattac ctcgacgcca agtcacagca gaccgagagc gaatggcagc gcctcgcaca
     8761 ggaagcttcg ctcagcgaga aacaaaaacg tatcctgtat gatttcctga aaaaagagca
     8821 gcgccgtgcg ctttcatcac accgcgaaat gattctcgac ctgatggctg gcgcgggcaa
     8881 agtacgacag gcttgagcgt gcctgcaaaa gaaatcacac tcgcctcgcg cgattcgcta
     8941 cttgccctga cacagacgat cgaggcagca atgcggttag aagctgcggg ttttgccccg
     9001 cgtatcgtca gcatgaaaac ggcgggcgat atcaagctga acgccccgct ctattccgta
     9061 gcacaacaat cgggcaacaa agaaggcagg gcatttttta cccgcgaact cgatgacgcg
     9121 ttgctttccg gccgggctga tgctgccgta cattcattca aggacctgcc gaccgaaaaa
     9181 gtgcccggga tcggcgagcc gtactttttc tgcgaagagc atggctctga tgtactgctg
     9241 ctcggtgaaa aaactgttat gagtactgac ggcgctgggc tggtcatagg cacctcgtcg
     9301 ctgcggcgca ttcaccagct gcaactcgtt cttcccgcgg cgcgtacagt tactctgcgc
     9361 ggcaacgtta tcacgcgact cgaaaaactg ctcgcgggag aaaatgaaat caacgcgatc
     9421 ctgattgccg cggccgggct taggcgcctg acggcttttg cggaagtacc cgagaacagg
     9481 tatgcccacc tggttgtgcc tgcggcgctc gaacacattc gccgcgaact cgcccgcctg
     9541 gccgcgagcg aagcagcgcg cgccagagca atcgaactgc ctgaacgtta ttttcccaca
     9601 gcccccggac agggtgtgct ggcgctgcag ctttctgcgg aagccgaagc ccgctactcc
     9661 ggcgcagtcg ccgccgtatt tgccgaacac cagcagattg cccgccgcgt gatgcaggaa
     9721 cgccggatta tgaccgaact catgacaggc tgccatgcgc cattgggagt ttccgccctg
     9781 cggggtgacg agcaccgtat cgttgcctgc tacagccgaa aatccactac cgagccggtc
     9841 agtttttccg attcggtctg gtttgaacgc gagctgggcg cagacagtac ggcaatcgcg
     9901 caggaactca aactcccccg ggaacaaatc tactggtggg gctttaaggc accgccgcaa
     9961 aattccgggc tgccattgca atttgtacca gccgtggagc agaagccact ggaggtgcag
    10021 tggtcgggcg cgttacccga agcagtgttt gttgcatcgc cgcgcgcggc tgagtgggta
    10081 tcggcgcaaa aggacctgtt gaatttaccg ttctatactg ctggtgcaga aactggtcat
    10141 gtattgaagt cgcttatccc ggctgcacaa atcacccccg tagccggcaa aggattcgcg
    10201 caggcactga aagtatttag taacaagcag gtacagctgc tgtgggtcgg ttcagccgat
    10261 ggcgaaaaac gtgcccgtgc tacggcgcag gaatatcctg ctgcgagatt tttgcctgtc
    10321 tatacgaacc ttccgctaag gccaaaacta cccgctcctg tagcgggtgc actgcacgtt
    10381 ctcacttcgg caatggctgc tgaagccttc gtgcgctggg cggccgaagc tggcaatatt
    10441 gacccgcaaa tctgctgctt cggtgaatcg gcggtcgaga tcgtcaaggc tactggtatg
    10501 ccagtttacc atgccagcga ggcgaacgat ttctctgctc ttgttcgtga attaaaggga
    10561 gatacaggcc tgctgcgcga acgctggcaa ctcaaggagt tctgacccat ggaaagaaaa
    10621 aaatctgaca aactctggca acgcgccctt aaggttttcc ccggcggggt caactcgccc
    10681 gtacgcgcct tccgctcagt cgggggaaat cccctgttca ttgcgagcgg caaaggcagc
    10741 tacgtgcgcg acgccgatgg caaccgctac ctcgattttt tgtcgagctg ggggccgctg
    10801 attctcggtc atgcgccgag gcccgttgtg cgggcaacac gcaaggcgct gcggcatggt
    10861 tcgactttcg gcgcaacaac cgaagcggaa gtaaaacttg gcgagtttat cctgcagaat
    10921 attccgtttc tcgaacgggt gcgctttgtc agctcgggta ccgaagcggt gatgaccgcg
    10981 ctcaggcttg cgcgcggcac cacgcggcgg cagatgatcg tcaaattcga aggctgctac
    11041 catggccata acgacgcact gcttgcccgc gctggctcgg gattactgac actgtccggc
    11101 aatattgccg aagcaagttc acccggtatc cccgacgaaa tcgtgcagaa cactcttgta
    11161 ctgccactcg atgactctgc cgcatttgaa caggcattgg aaaaacacgg cgaaaaaatt
    11221 gcctgcgtca ttatcgaacc cgtccccgcc aatgcagggc tcttgctgca gcgccgggca
    11281 ttcatcgagc acgtgctcac ctcggcacgc cagaagggta tactggtgat cttcgacgaa
    11341 gtcatcagtg gcttccgcct gtcctttgcc ggctatgcag gtcgcgaaaa cctgaagccc
    11401 gatatcatta cctatggaaa aattatcggc ggcggtc
//
LOCUS       DAOQJF010000067        11417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2866_length_11417_cov_4.817638, whole genome shotgun sequence.
ACCESSION   DAOQJF010000067 DAOQJF010000000
VERSION     DAOQJF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_11417_cov_4.817638"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..250
                     /locus_tag="PLF85_05270"
     CDS             50..250
                     /locus_tag="PLF85_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19104.1"
                     /translation="MKQLLRMYVYLPLTLLFFGFAAHRMLTQAGGKEPIEAENLNVKN
                     IRANPTSFRAVYMPRILAGGGK"
     gene            288..575
                     /locus_tag="PLF85_05275"
     CDS             288..575
                     /locus_tag="PLF85_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNE19105.1"
                     /translation="MKKIFFLLFVATAVFAENGEMPKVKALLDLEHILAVLVYSTIGI
                     LVMVLSFAIIDWLHPRDIWGEIADKQNRAMAIMIGCMMLGIAIIIAAAVVG"
     gene            581..2722
                     /locus_tag="PLF85_05280"
     CDS             581..2722
                     /locus_tag="PLF85_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNE19106.1"
                     /translation="MAQSGAPRRRWLRALLVVFLLLAAAFAGGFFYLRNRISPERISA
                     EASALLRRKLGFALSIGELRLTWTGNAELRRICVRNEAMLSQRCLFSADIVQLDLRLL
                     PLLRRQLEIRGAQLENCELNLFSETAETPDKKKITRHSWEGSVTPVTEPAPGAGTAGT
                     SQMSVRLDNLAIRNAVIAHEAQALPIPLGKTSVSLGLRHAGERLELKAQLPDSSQLTA
                     DLHLKLADFFATARLVVAGPRLADSDQVSGTIECERCNLSGFESRAGFLTGKLKAEAV
                     AKELRLQGDNVQVTTRSAYAPALTLNGNAAIALPELYPIAGEGSIAGQGLTVQYRKLA
                     GSQKGGVSVEFDLAADLSRLPGISGMRGTAGVHGQISNHSTSAGFSLRNFSTAAAGIA
                     VSAEQLNGKLLGQRVSLRKQGVVLGGNAAEITLDADTGSRPTSVQGSVAFAELNLDKF
                     MAGEKNNAAAQAQPAQPAAGTSPSVKASIQLSAGALQIGKLRTGPLRAQVLSDGKTTD
                     VRSWQVEFGQGRMQGSYQQHADGKQDLSFRVLAVKAQNLNELLGFKATVYGTIDADGR
                     LAFAGSSPDSVLKTGTGQLSFRMGRGKIRDSFLQKGVLSGPLHKLEEKFSDIEFASAQ
                     AEVRLSPDKIQVQKFYFDAEEWNVTYRAEADAAWQGKAALAFRFRSSFVENVANPLHM
                     GISDRKDGDFYDLPFACRGAVLTGTCYKKNW"
     gene            complement(2737..3960)
                     /locus_tag="PLF85_05285"
     CDS             complement(2737..3960)
                     /locus_tag="PLF85_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE19107.1"
                     /translation="MSANLRLLANKSFLGFIGFRTSMVLGFQVVGTIAHWQVYAITRD
                     SIFIGYLALTEVVPAIALSLLGGYWADSFNRRKVALIGSSITLLSITILVSGAAANEP
                     SSILPLFIAVAIIGVGRGIVAPASTALLGEIVDRQFYARAAVFNSIGWHMALITGPAM
                     AGILYEKYSPLLCYRIALILTILSIVFLWFIHAPGRPANRREKISVAIREGLKFVFHT
                     RIVLGAMSLDMFAVLFGGAVALLPAFSDVIYKTGAEGLGYLRAAPAVGAAIMGLILIF
                     VPIRHHAGKVLLVAVALFGISMIAFGLNTDFTRALFILALSGGVDNISVVMRSTIMQT
                     HTPDPMRGRVAAVNSIFIASSNEIGAFESGFTAKFMGLVPAVVFGGSMTIVVVVMIAL
                     LIPELRRLSFAGTQK"
     gene            complement(3986..5572)
                     /locus_tag="PLF85_05290"
     CDS             complement(3986..5572)
                     /locus_tag="PLF85_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19108.1"
                     /translation="MNKKLLVLTLLLAGNTLFADRWLILPPRIENTAGGESLGASAQA
                     GTTAADLARAMALYLRASRVSHVVSVAEAEGCLKQENLSMEQKIAPELIGRVAKNCQA
                     ERVLLTRVRRRSGEFEVTSKVYFRESDTLTDTLVKSGDDLQRVIGEQLSERFSARPAT
                     PKESSRDLVVAGDTFGAAYFDWQQLKPFFLSLDSVKSAYCLLDSQGKLQTYRLRADKQ
                     QEKEFIERLRFEGGYSLQNPQAMTDCLRKAQSETQREGRRAVVVLLVSAQPQDAEARL
                     QLKAALRQIAARNKLLIVPTGSATEAAARFWAQITRELGDDAQYLPTAQRARAGLSSG
                     QEWFIFRRGGRLFESRSAEPQKLAGGVLIPEKFSDMNAPQDLLKLYAQLSGNKVVSAG
                     EATAWNGALTSALAQSFQTTGSGGSWRVMLEQNGQSYYLSLAAPEARKLKVGEYARIY
                     TELLPGSDREVLRNRPSPVVIIEKAHDSAASIELNVTDFLRQPARYLRRGIGGRSFYI
                     MTGKVLRVSPPERDAIDDGF"
     gene            complement(5588..6700)
                     /locus_tag="PLF85_05295"
     CDS             complement(5588..6700)
                     /locus_tag="PLF85_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE19109.1"
                     /translation="MAKKVTVKKVPAVATGPTLLVAAGGTGGHINPGISIAEEWLRSG
                     GRVILATLIKNVEYPDIIRLARNEAVSIVAYDAPRLVKNPLKLWGFFKRFRSAYNLVR
                     QASKAEHTAGIVGMGGYSSFPAVVYALFNRLPLFLCEQNARWGIVTRLARYFARRIFL
                     SFPATVKLPQKFIVTGNPLRSVFQHAKAPQKNAARTRTIFFVGGSQGAHDINALYLAF
                     CAKPEAKNYRCIVSTGAQQHAEISRAARRGDDIRPFIVDMPETLLAADFVVARCGSGT
                     LSELVWAQKPAFLIPFPFAAANHQRANADAVIDELDCVVFDERPFKAEAALTKLLGFL
                     QSRTQAKGAASVAPTAAKANASAAQREIVRYIKEEL"
     gene            complement(6701..7933)
                     /locus_tag="PLF85_05300"
     CDS             complement(6701..7933)
                     /locus_tag="PLF85_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE19110.1"
                     /translation="MNAFEVLKALTSLPLDAEAQHLLEWELYWYRPAGADAAFAEDSK
                     RETLPLRLSVSEETFARMKSSVDHTEADEHGITIPLAEAGRQPESLQVSTRAQQIIPD
                     GVHYSATQLSAEGDLTLKDALLLCRLVARFPQLTVEASMAPADKTDLDALSQSFRRTI
                     FRGIIMGRRPSAGLRLLDRFGLLEYFLPEVTAGRGLTQNRFHAHDIFEHLLRSVDGAM
                     DLNEAVRWSALLHDIGKVPTRVETDTGEATFHNHEMYSARMVVPIMKRLAVPITVGQK
                     VKFLVRNHMFHYTDEWSDKAVRRFVKKVPLEELQDLISLRLADRKGSGKKTAFPKALQ
                     KLIDHIDEIIAKEQEFKIKDLAVNGHTLMEMGIPASRQMGDILKYLHAEVAAGRAANE
                     TEILVSMVRQKMTEQKAE"
     gene            complement(7942..8754)
                     /locus_tag="PLF85_05305"
     CDS             complement(7942..8754)
                     /locus_tag="PLF85_05305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE19111.1"
                     /translation="MTCPLCGTSLAALSDKRFGVCSKDGLVVNGAYEFRRYEHDYFEQ
                     EYRNQYGRSYTEDRQAILARNEIRFAKLKELAPVATHPQVLEVGSAAGYFLKIMQEAG
                     YAVRGWEISEAMTKYSNARGIKTVRQDFLRGAALHEKQQKQPYDIVAMFYVLEHIPEQ
                     KLAWQKLAGLVRPGGYLLLALPSAGGPTFHFHRQRWYDTHPLDHSVDYSPRSLKLAGR
                     QFGFKLVSAFSEGIHPQRFPLGAYRLPGRLYRAALEHAPLGDTIFAILLREQ"
     gene            complement(8801..9817)
                     /locus_tag="PLF85_05310"
     CDS             complement(8801..9817)
                     /locus_tag="PLF85_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19112.1"
                     /translation="MVKLRLLLILLTLRPVIADITITSTTVNLSAANAQLVGKLDNTL
                     NDLFYSIASLAGPGIINAAAFSSTIGIQRQQENMPRFQLEPSAGIIVPGKAKGDERLG
                     ALPLYAANIVGGFRLDEKTAIQLRGFYLPETEFNVRTTRLSIQPYSFGASLTRQVKAA
                     GSEWYNPAIVTPLDLAYMHGTFAATFSGTAKDVSFDPAGDGSQGNARADVHYINNFRL
                     RWNVYSFTTGLILVKPFLSVFTARLGLLASLHLGNSTLTNTANGTMLVTASAATGGNE
                     FKVNDTATIEVRNAASFRPVLVSNQVSLGLGINLAGATLNIDLTQNLQYNATAIVLQL
                     GCWL"
     gene            complement(9811..10479)
                     /locus_tag="PLF85_05315"
     CDS             complement(9811..10479)
                     /locus_tag="PLF85_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19113.1"
                     /translation="MFRGLALSKTLRFAVICTVSLAGSHQIFAQRTGEWQVAVMTGFN
                     SVAAGKESLLADNRGTELGLYVTTPWQLWSAHYTALSLRAQAVTYPNAIETPSPAVAG
                     ETVKLTNAVHSQLRADIRQIYEIWDIHWTLGLGLMLPVTNSISTPRGEFSFADARTEY
                     PQATDSLRKIDRSYAVFLRVGIDQKILNDMLLLGLGFDISALEFPRTAQLVSFNLYAG
                     VSVW"
     gene            10589..>11417
                     /locus_tag="PLF85_05320"
     CDS             10589..>11417
                     /locus_tag="PLF85_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19114.1"
                     /translation="MLHLLSGARVKINARLVSALVLASSLWTISPLAALEVFLNRSFT
                     SEERPQATLYASGEGTLFLRLYRIDDVHGYLSGQGNAHTVTEKNERLMQPGYFLWRSV
                     VENLEYSFYQLARRYMRADYRERLRADLGLEKYAFPFRDRFPETNLFSALPYPIVAEQ
                     AIAVRARHWQALKHEFAQLAPGYYLVEASQGRHIAHSPLVVSDIAMVTKTSPHNILVY
                     AINLKTGEPLSDGMVTAYVRAKQAHQQKLSFPLKEGIAFTEKTGFLNGAESTVYVLEH
                     "
BASE COUNT         2390 a         3162 c         3418 g         2447 t
ORIGIN      
        1 gaacatttac tactacatcc gtttccgcat aaaagagggg gagcgataaa tgaaacagtt
       61 actgcgcatg tatgtctacc tgcctctcac cctgctgttt tttggctttg ccgcgcaccg
      121 tatgctgaca caggccggtg gcaaagagcc gatcgaagca gaaaacctga atgtgaagaa
      181 tatccgcgcg aacccgactt cgtttcgcgc ggtctacatg ccgcgcatcc tggcaggtgg
      241 tggcaaatga cgtactatag gaattcagtt aattggggag taaacatatg aaaaagattt
      301 tctttctgct atttgtggca accgcggtat tcgcggaaaa cggagagatg cccaaggtga
      361 aagcactgct cgacctggag cacatcctgg ctgtcctggt ctattcaacg atcggtatac
      421 tggtgatggt gctttcgttc gcgattatcg actggctgca cccgcgcgac atctggggtg
      481 aaattgccga taagcagaac cgcgcgatgg cgatcatgat tggttgcatg atgctcggta
      541 tagcgatcat catcgcagca gctgtagtag gctagttcgc atggcgcaga gcggcgcgcc
      601 gcgcagaaga tggttgcgcg cgctgctggt agtttttctg ttgttggcag ctgcctttgc
      661 gggtggcttt ttttatctca ggaaccgcat ttctcccgag cgaatttcag ctgaagcatc
      721 ggcgctgctg cgccgcaaac tagggtttgc gctctccatc ggcgagctgc gcctgacctg
      781 gacgggtaat gctgagctcc ggcgcatttg tgtacgcaac gaagcgatgc tttcgcagcg
      841 ttgcctcttt tcggctgata tcgtgcaatt agacctgcgc ctgctgccac tgttgcgcag
      901 gcagcttgag atcaggggag cgcagctgga aaactgcgaa ctcaacctct ttagcgaaac
      961 tgcagaaact cctgataaga aaaagattac ccgccacagt tgggaaggct ctgtaacgcc
     1021 tgtgaccgaa cctgcgccgg gtgccggaac tgcggggaca tcgcagatgt cggtgcggct
     1081 cgataacctg gcgatcagga atgccgtgat tgcccatgag gcgcaggcgc tgcctattcc
     1141 gctgggtaag accagtgtct cactgggtct gcgtcatgcc ggcgaacgcc ttgagctgaa
     1201 ggcgcagcta cccgacagca gccagttaac agctgacctt catctgaagc tcgcggattt
     1261 ttttgccacg gcgcggctgg ttgtagcggg gccgcggttg gcagattctg accaggtcag
     1321 cggcaccatc gaatgtgagc gctgcaatct ttctggtttt gagagccgag caggtttcct
     1381 gacgggcaag ttaaaggcag aggctgtggc gaaagagctg cgcctgcagg gcgataacgt
     1441 gcaggtcact acgaggtctg cttatgcgcc agcactcacg ctgaacggca acgcggctat
     1501 tgccctgcct gagctttacc ccattgcggg tgaaggcagc attgcgggtc aggggctgac
     1561 cgtccagtac cggaaactcg ccggttcgca gaagggcggt gtgtcggttg aattcgactt
     1621 ggcggccgat ctttcgcgcc tgccgggcat atcgggtatg cggggaacag caggggtgca
     1681 tggacagatc agcaatcact ccacctcggc tggtttctca ctgcggaatt tttctacggc
     1741 cgcagcgggc atcgctgttt ctgcagaaca actgaatgga aaactgttgg gccagcgcgt
     1801 cagcctgcgc aagcagggcg tggtgctcgg cggtaatgcc gcagaaatta cgctcgatgc
     1861 cgacacaggc agtcgaccga ccagtgtgca gggcagtgta gcttttgcgg agcttaacct
     1921 cgataagttc atggccggcg aaaaaaacaa cgcagctgcg caggcacagc cggcgcagcc
     1981 agctgctggc acgtcgccgt cagtaaaggc cagcattcaa ctttcggcgg gagcgctgca
     2041 aatcggtaaa ctcaggaccg ggccgctgcg cgctcaggtt ttgagcgatg gcaagacaac
     2101 cgatgtccgc tcgtggcagg tggagttcgg gcaggggcgg atgcagggca gctaccagca
     2161 gcatgcggac ggcaaacagg atctttcgtt tcgggtactg gctgtcaaag cgcagaacct
     2221 gaatgaactg ctgggattca aggcgaccgt ctacgggacc atcgacgcgg acgggcggct
     2281 ggcatttgcc ggcagtagtc ctgattccgt cctgaagaca ggcacaggcc agttgagttt
     2341 tcgcatgggt cggggcaaga tacgcgacag tttcctgcaa aagggagtgc tgtcagggcc
     2401 gttacacaag ctcgaggaaa aattctctga tatcgaattt gcctctgcgc aggcagaggt
     2461 ccgtctgagc cctgataaga tacaggtaca gaaattctac ttcgacgcgg aagaatggaa
     2521 tgtcacgtac agggctgaag cagatgccgc gtggcagggc aaagctgcgc tcgcgttcag
     2581 gtttcgttcg tcgttcgtag agaacgtcgc gaacccgctg cacatgggca tcagcgaccg
     2641 caaagacggc gacttctatg acctgccgtt tgcctgccgc ggtgcagtgc tgaccggcac
     2701 ctgctacaag aagaactggt aactatagaa tttctcctat ttctgcgtgc cggcaaaact
     2761 tagccggcgc agctcaggaa tcagcaaggc aatcatcacc accacgacaa tcgtcataga
     2821 accgccaaaa actaccgcgg gcacgaggcc cataaatttc gcggtgaagc cgctctcaaa
     2881 ggcgccaatc tcgtttgatg aagcaatgaa gatcgaattc accgcagcga cgcggccccg
     2941 catcgggtca ggcgtgtgcg tctgcatgat ggtcgagcgc atcaccacgc tgatgttgtc
     3001 caccccgccg gatagagcga ggataaacag ggcacgggta aaatcggtgt tcaggccgaa
     3061 ggcaatcatc gaaataccaa aaagtgccac cgcaacgagc aataccttgc cggcgtggtg
     3121 cctgataggt acaaagatca gtatcagccc cataatcgcc gcacccactg ccggggctgc
     3181 gcgcaggtaa cccaggccct cagcgcccgt cttgtagatg acatcgctaa aggctggcag
     3241 caatgccaca gcgccgccga agagcactgc aaacatgtcg aggctcattg cccccagcac
     3301 aatgcgtgtg tgaaaaacga acttaaggcc ctcgcgtatg gcgacggaaa ttttttcgcg
     3361 acggttggcc ggcctacctg gggcatggat aaaccagaga aacacaatcg acaggattgt
     3421 aaggatgagc gcgatgcggt agcacagtag cggtgaatac ttctcataga gtataccagc
     3481 cattgcaggt ccggtgatca gcgccatgtg ccagccgatg gaattgaata cggcggcacg
     3541 ggcatagaac tgccggtcga cgatttcgcc gagcagcgcg gtgctggcgg gtgcaacaat
     3601 acctctgccc acgccaataa tggccacggc tataaaaagc ggcagtatgc ttgaaggttc
     3661 attcgctgct gcgccgctca cgagtatcgt gatgctgagc agcgtgatcg aagaacctat
     3721 tagggcaacc ttgcggcgat tgaagctgtc ggcccagtag ccgccgagca ggctgagcgc
     3781 aatggcagga acaacttccg tcagggcaag atagccgata aaaatggaat cgcgggtaat
     3841 cgcgtaaacc tgccagtggg caatcgtacc caccacctgg aaacccagta ccatcgaggt
     3901 acgaaagcca atgaagccga gaaaactctt gttggcgaga aggcgaagat tggcactcat
     3961 ctcaggcttc ccgggtcact cgtggttaga acccatcatc gatcgcatcg cgttcgggtg
     4021 gggaaaccct cagcacttta ccggtcatga tgtagaacga tcggccgcca atgccgcgcc
     4081 gcaggtacct ggccggttga cgcagaaaat ccgtcacatt gagctcgatc gatgcggcgc
     4141 tgtcgtgcgc cttttcgatg atgacaaccg gtgaaggccg gttgcgcagg acttcgcggt
     4201 ctgagccggg cagaagttcg gtgtagatgc gcgcgtattc acccactttg agttttcgcg
     4261 cttcaggcgc tgcgaggctg aggtaatagc tttgcccgtt ctgttcgagc atcacacgcc
     4321 agctgccgcc cgagcccgtt gtctggaatg actgcgccag tgcactggtg agcgcgccgt
     4381 tccaggctgt ggcttcacca gcagaaacta ccttgttgcc cgagagctgc gcataaagtt
     4441 tcagcagatc ttgcggcgca ttcatgtcag aaaacttttc gggtatcagc acaccgcctg
     4501 ccagtttctg cggttcggcg gagcgactct cgaacagcct gccgccacgg cgaaagatga
     4561 accattcctg tccgctcgaa aggcctgcac gcgcgcgttg cgcggtgggc aaatactgcg
     4621 cgtcgtcgcc cagttcgcgc gtaatctgcg cccagaaacg cgctgcggct tcggtggcgc
     4681 tgcctgtcgg tacgatcagc agtttgttcc tggctgcaat ctgccgtagc gcagccttga
     4741 gctgcagccg cgcttcggca tcctgtggtt gcgcggagac gaggagaacc accacggcac
     4801 gccggccttc gcgctgcgtt tcagactgcg ccttgcgcag gcaatccgtc atggcctgcg
     4861 gattctgcag cgagtagccg ccttcgaatc gcaggcgttc gataaattcc ttttcctgct
     4921 gcttgtcggc ccgtaacctg taggtctgca gtttaccctg agaatcgagc agacagtagg
     4981 ccgatttgac gctgtcgagg ctcaggaaaa acggtttgag ctgctgccag tcaaagtacg
     5041 cagcaccgaa ggtgtcgcct gccacgacca ggtcgcggct cgattctttg ggcgttgcgg
     5101 gacgcgcgct gaagcgttcg ctgagctgct cgccgatgac gcgttgcaga tcatcgccgg
     5161 atttcaccag agtatcggtc agcgtgtcag attcgcgaaa atatactttg ctcgtgactt
     5221 caaattcacc cgaccggcgg cgcacacgcg tcagcaggac acgctccgcc tggcagtttt
     5281 tggcaacgcg cccgatcagc tccggagcaa tcttttgctc catgctgagg ttttcctgtt
     5341 tcaggcagcc ttcggcttct gctacggaaa caacatgcga aacgcggctg gcgcgcaggt
     5401 agagggccat agccctcgcg aggtcagcgg cggtcgtacc cgcctgcgct gaagccccca
     5461 gcgattcacc acccgcagtg ttttcgatgc gtgggggtaa aatcagccag cggtcggcaa
     5521 aaagggtgtt gcccgccagc aagagggtga gaaccagaag tttcttattc atgcgcgtgt
     5581 ccgatgccta aagttcttct ttaatatatc ggacaatctc gcgctgtgcc gcagaggcat
     5641 ttgccttcgc cgcggttggc gccacggacg ccgcgccctt cgcctgcgtg cgcgattgca
     5701 ggaaacccag cagcttcgtt aaggctgcct cggccttaaa ggggcgctcg tcaaagacca
     5761 cgcagtcaag ttcgtcgata accgcatcgg catttgcccg ctggtggttt gccgcggcaa
     5821 aagggaaagg gatcaggaat gcgggtttct gtgcccaaac gagctcagac agcgtaccgc
     5881 tgccacaacg cgcaacgacg aagtcagccg caagcagggt ctcgggcata tcgacgatga
     5941 aggggcgaat atcgtcaccg cggcgcgcgg cacgcgatat ttcagcgtgc tgctgtgccc
     6001 cggtagacac aatgcagcgg tagttcttcg cttcgggttt ggcgcagaat gccaggtaaa
     6061 gcgcgttgat gtcgtgtgcg ccctggctgc cgccgacgaa gaaaatggtc cgtgtacggg
     6121 cggcgttttt ttggggagct ttggcgtgct ggaaaacaga ccgcagcgga ttaccggtca
     6181 cgatgaactt ctgcggcagc ttcacggtgg caggaaacga caggaagatt ctgcgggcaa
     6241 agtagcgcgc gagccgggtt acgatacccc agcgggcatt ctgctcgcag agaaagagcg
     6301 gcagccggtt gaacagtgca tagaccaccg ctgggaacga actgtagccg cccatgccca
     6361 cgatgcctgc tgtgtgttca gccttgctcg cctggcggac caggttgtaa gccgaacgga
     6421 agcgtttgaa gaatccccag agtttcaatg ggttctttac gaggcgcggc gcatcgtacg
     6481 cgacgatgga gacggcctcg ttgcgggcga gccggatgat gtcggggtac tcgacgttct
     6541 tgatcagggt tgccagaatg accctgccac ctgagcgcag ccattcttcg gcgatgctga
     6601 tacccgggtt gatgtgcccg ccagtgccac cggcagcaac cagcagtgtc ggccctgtgg
     6661 caactgccgg gacctttttt actgtaactt tcttcgccat ctattctgcc ttctgttcgg
     6721 tcattttttg ccgcaccatc gagaccagta tttcggtctc attggcggcg cggcccgcag
     6781 ctacttcggc gtgcaggtat ttcaaaatgt cgcccatctg ccgtgacgcc ggaattccca
     6841 tttccatcag ggtgtggccg ttgactgcaa gatcttttat cttgaactcc tgctctttgg
     6901 caataatttc gtcgatgtgg tcgatcagct tctgcagcgc cttgggaaat gcggtcttct
     6961 tacccgaccc cttgcggtct gctagcctga gcgagatgag atcctgcagc tcttcgagtg
     7021 gcaccttttt cacaaagcgg cgaacagcct tatctgacca ctcgtcggta tagtggaaca
     7081 tgtggttccg caccaggaac ttcaccttct gcccgacggt aattggtacc gcgagccgct
     7141 tcatgatggg cactaccatt cgcgccgagt acatttcatg gttgtggaat gtcgcttcgc
     7201 cggtatcggt ctcgacacgt gtcggcacct tgccgatgtc gtgcaggagg gccgaccagc
     7261 gtaccgcttc gttgaggtcc attgcgccat cgaccgagcg cagaaggtgt tcaaagatat
     7321 cgtgcgcgtg aaagcggttt tgcgttaaac cgcggcctgc cgtgacttcg ggtaagaagt
     7381 attcgagcag accaaagcgg tcgagcaggc gcaggccggc cgatggccgc cggcccatga
     7441 tgatgccgcg aaagatggtg cgacggaacg actgcgaaag tgcatcgaga tcggtcttgt
     7501 ctgcaggcgc catggatgcc tcgaccgtga gttggggaaa acgcgccacc agccggcaaa
     7561 gcagcagcgc atctttcagc gtgagatcac cttcggcgct gagttgtgtg gcagaataat
     7621 gcaccccgtc aggaatgatc tgctgcgccc tggtacttac ctgcagcgac tcgggttgtc
     7681 ggccggcttc tgctaacgga atggtgatgc cgtgttcatc cgcctcggtg tggtcgaccg
     7741 aacttttcat gcgcgcgaac gtttcttcgc tgaccgaaag tcgcagcggc aaagtctcgc
     7801 gtttgctgtc ttcggcaaat gctgcatctg cgcctgccgg ccggtaccag tagagctccc
     7861 actccagaag gtgttgcgct tcggcatcga gcggcaggct tgtcagtgcc ttaagtactt
     7921 cgaaggcatt catgatcgaa attattgctc ccttagcaga atggcgaaaa tcgtgtcgcc
     7981 gagcggtgca tgttccagtg cggctcggta cagcctgccc ggcaggcgat aggcgcccag
     8041 cggaaagcgc tgcggatgga tgccctcgga aaaggcagaa acaagcttaa agccaaattg
     8101 ccggccggcg agtttgagcg accgtggcga gtaatctacg ctgtggtcga gcggatgcgt
     8161 gtcgtaccag cgctggcgat gaaaatgaaa agtgggacca cctgccgagg gtaatgccag
     8221 taacaagtag ccaccggggc gcacaaggcc ggcaagtttt tgccaggcga gcttctgttc
     8281 tggtatgtgc tcgagcacat agaacatggc gacaatgtcg tacggttgtt tctgctgctt
     8341 ctcgtgcaga gcggcacccc tgaggaaatc ctgccgcacg gttttgatgc cccttgcatt
     8401 cgagtacttt gtcatggctt cgctgatttc ccagccgcgt accgcgtaac cggcttcctg
     8461 catgatcttt aggaaatagc ccgcggctga accgacctcg agcacctgcg ggtgcgtcgc
     8521 gacgggggcg agctctttca atttcgcaaa gcggatttcg ttgcgtgcaa ggattgcctg
     8581 ccggtcttcg gtgtagctgc ggccgtactg gttgcggtac tcctgctcga agtagtcgtg
     8641 ctcgtaacgc cggaattcgt acgcgccgtt taccaccagg ccgtctttgc tgcagactcc
     8701 aaagcgtttg tctgacaggg cagcgagaga agttccgcag agcgggcagg tcacaagggt
     8761 gggtcagggt atttgcacag gctgaaaaac aaaaatcagc tcagagccag caacccagct
     8821 ggagaacaat tgccgtcgca ttatactgca ggttttgagt caggtcgatg ttcagggttg
     8881 cacctgccag gttgataccg agtccgaggc tcacctggtt ggaaaccagg acggggcgaa
     8941 aactggctgc gttgcgcacc tcaatcgtgg cggtgtcatt cactttgaat tcgttaccgc
     9001 cagtcgccgc cgaggcggtc accagcattg tcccgttagc ggtgttcgtg agagttgagt
     9061 tgccgaggtg cagcgaagcg agcagcccca gcctcgctgt gaagacagag aggaacggct
     9121 tcaccagtat gagcccggtg gtgaatgaat agacattcca ccgcaacctg aagttgttta
     9181 tgtagtgcac atcggctcgt gcattgccct gcgaaccgtc gccggccggg tcgaagctga
     9241 cgtctttcgc cgtgccgctg aaagtggctg caaatgtgcc gtgcatgtac gcgagatcga
     9301 gcggcgtaac gatcgcgggg ttataccatt cgctgcctgc tgccttgacc tggcgcgtca
     9361 gggaggcgcc gaagctgtaa ggttgtatgc tgagcctggt tgtcctgacg ttaaattccg
     9421 tttctggcag gtaaaaacca cgcagctgga tcgccgtctt ttcatcgagg cgaaatcccc
     9481 cgacaatatt tgccgcgtac agaggcagag ctcccagccg ttcatcgccc ttggctttcc
     9541 ccgggacaat gatgcccgca gagggttcga gctggaagcg cggcatgttt tcctgctggc
     9601 gctggatgcc gatggttgaa gagaacgcgg cggcattgat gattccaggg cccgccagtg
     9661 aagcaatgct gtagaagagg tcgttcagcg tattgtctag tttgccgaca agctgcgcgt
     9721 tggctgccga taggttgacg gttgtcgagg tgattgttat atcagcaata acgggtcgaa
     9781 gtgtaagtaa tatgagcagc aggcggagct tcaccataca cttaccccgg cataaagatt
     9841 gaaactgacc agttgcgctg tacgtggaaa ttccagcgcg ctgatatcga accccaggcc
     9901 cagtagcagc atatcattca gtattttttg gtcgataccg acgcgcagaa atacggcgta
     9961 cgaccggtct atcttgcgca ggctgtcagt agcctgcggg tactctgtgc gtgcatcggc
    10021 gaagctgaat tccccgcgag gcgtggagat gctgttggtc accggtagca ttaggccaag
    10081 ccccagggtc cagtggatat cccagatttc gtatatctgc cgtatgtcag cgcgcagctg
    10141 cgaatgtact gcgttggtga gcttcacggt ttcgccggct actgccggcg agggggtctc
    10201 aatggcatta ggataggtca ctgcctgcgc acgcagtgaa agcgccgtat agtgcgcgct
    10261 ccacagctgc catggggtcg tcacgtagag accgagctct gttccacgat tgtcggccag
    10321 caatgattct ttgcctgccg ctaccgagtt aaagcccgtc atgaccgcta cctgccattc
    10381 gccggtgcgt tgcgcaaaaa tttgatgcga accggccagg cttaccgtgc aaattactgc
    10441 aaaccgtagg gtcttactga gggccagccc tctgaacatg gcgcagacac gaagaaacag
    10501 gatgcgccgg caagcgggta aatgccctgt ggtctgaatt ttgctccctt tctaattgac
    10561 ggcatgcgtg gccgaccaag gcgtctgtat gctgcacctt ctttccggcg cgcgggtgaa
    10621 aataaatgcc cgtttggttt ctgcgctggt cctggcctca agcctgtgga caatttcgcc
    10681 actggccgcc cttgaagttt ttctcaaccg gtcgttcacc tcggaagaaa gaccacaggc
    10741 tacgctctat gcttcgggtg aaggcaccct gtttctgcgt ctctaccgta tcgacgacgt
    10801 gcacgggtat ctcagcgggc agggcaacgc gcacacggtg accgaaaaga acgagcgcct
    10861 gatgcagccg ggctactttc tctggcgcag cgtggtggaa aaccttgagt attcatttta
    10921 ccagctggcg cgccgttaca tgcgcgccga ctaccgcgaa cgcctgcgcg cagacctcgg
    10981 tcttgaaaaa tatgcattcc cgttccgtga ccgcttcccg gagaccaacc tgttctcggc
    11041 gctgccatac ccgattgtag cagagcaggc gattgccgtg cgtgcgcgcc actggcaggc
    11101 gctgaagcac gagtttgccc agcttgcgcc cgggtattac ctcgtcgagg cctcgcaggg
    11161 ccggcatatt gcacactcac cgctggtggt ttccgatatt gcgatggtga cgaaaacttc
    11221 gccgcataat attctggttt atgccattaa cctcaagacc ggtgagccgc ttagcgacgg
    11281 tatggttacg gcttacgtgc gcgcgaagca ggcacaccag caaaaacttt cgtttcccct
    11341 gaaagaaggc attgccttta ccgaaaaaac tggttttctc aacggggccg aaagcaccgt
    11401 ctacgtgctc gagcacg
//
LOCUS       DAOQJF010000068        11368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2890_length_11368_cov_5.063378, whole genome shotgun sequence.
ACCESSION   DAOQJF010000068 DAOQJF010000000
VERSION     DAOQJF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2890_length_11368_cov_5.063378"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..721)
                     /locus_tag="PLF85_05325"
     CDS             complement(47..721)
                     /locus_tag="PLF85_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19115.1"
                     /translation="MRVRNLTLALLCCALFGLPVNAAPEKIFYAEAGTMNGFGLNAGF
                     GLHRIAPKFPIGFDFSAGYVFQSDPGNAVDARKIFINDATGGNDNIVESGATFFLGVD
                     ALYPIVRQSIFELNVAAGPRYSSYAAFFDYQGGNEAFKVYSNAWGFGVGFKSLLTISD
                     HFVIGLETTVEYFFNTQLKGHGFFYNANNQDDNPRNNYRYSDADAAINQPLFFPRVQL
                     LLGYKI"
     gene            complement(767..1219)
                     /locus_tag="PLF85_05330"
     CDS             complement(767..1219)
                     /locus_tag="PLF85_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE19116.1"
                     /translation="MNDRLRDILAADIFIDTNILFYGINIGNPEKYARAGALLEYLWQ
                     KEPLPSISTQVLQEFYVTLIRHRVEADEAQKIVKDYFVWNVIPAGIKLIEEAFQVQRR
                     YALSFWDSLIIAAAQRSQSEYLLTEDLNHGQKYGRLVVINPFHPDFAG"
     gene            complement(1212..1439)
                     /locus_tag="PLF85_05335"
     CDS             complement(1212..1439)
                     /locus_tag="PLF85_05335"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HNE19117.1"
                     /translation="MLRNITLRIDEDTLKKGKHIAVEQGLSLSKWVTELIEKSTDHAP
                     EIDKARSHALKTIRKGVSLGKGAFSRESVYE"
     gene            complement(1500..1988)
                     /locus_tag="PLF85_05340"
     CDS             complement(1500..1988)
                     /locus_tag="PLF85_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNE19118.1"
                     /translation="MAEPVQIITPALLDSLTERAKASERQRTNHNFHQSFEENPNRFL
                     NVMVKGTYVTPHRHTTPPKSESFVVLRGQLLFITFDDVGELQTVTRLSAQDNHGIDIA
                     PGVWHSIIVESDTAICFEVKPGPYAPANDKDFAPWAPREGEADCDEYADDVLQRAMRH
                     ML"
     gene            complement(1994..2959)
                     /locus_tag="PLF85_05345"
     CDS             complement(1994..2959)
                     /locus_tag="PLF85_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNE19119.1"
                     /translation="MKYLIVGAGAVGQVYGYHLVRAGADASFLIKERHAAAARDGFTL
                     YPLNGKDVRQNPVLFDKFSLLTDMADVAKQQWDYVFLTTSSTALRAGDWYAELAKAIG
                     NANVVILTVGIEDLAFVKRYTNEQNIITGLITMMSYSAPLPGEEVARPGTAYWFPPLL
                     KFTYSGEAQRTQAIVDLLNKGGMPSAKHNNVQLEGALGSALLQTSIYALECSGWSLKK
                     LAADKELLGITRKAMVEASFIGARKNNARVPYERTLIPGRALVGMMQVTQKIAPLDVE
                     RFFERHYTKVRDQSLDMLSAWIKAGAELDIKTPGLEALQKRLAVA"
     gene            complement(3013..4044)
                     /locus_tag="PLF85_05350"
     CDS             complement(3013..4044)
                     /locus_tag="PLF85_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE19120.1"
                     /translation="MENIKEVLSIAVGSHQEGFIIVADIPGRPIIYANQGFEKMTGFP
                     ATEIIGKNCNFLQGTETDPGSLEYLRKKLNEREACVVTLLNYKKDGQKFWNRLSIYPI
                     SVGPKNYFVGVQNDITMLVQTQQHFEILRKAQNLDNDRIHGLINVMRMLQDSIGNLMA
                     NLQYLSLEADQNGRLSPEAISLVDQIIDQTAEKFLFSSKEEFLGRFDFDRHLVQRQST
                     EKAPPRTHCLIVEDNLINQIILQKTLEGKGCKVTVANHGQEALEQLAKFKDFNVVFMD
                     IQMPVMDGIECTKRIRETVSTTLPVIAVTANTMAEDQALAREAGMNEFIGKPFEASQI
                     ERMLERFAS"
     gene            complement(4141..5088)
                     /locus_tag="PLF85_05355"
     CDS             complement(4141..5088)
                     /locus_tag="PLF85_05355"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNE19121.1"
                     /translation="MTETGTDILFAAALLRRGELVAIPTETVYGLAGNALDADAVLKI
                     YATKGRPQFNPLIVHLANKEAMATYAEVPPLAYRLAERFSPGPLTYVLPKKPIVPDVT
                     TGGGDTVALRIPAHAMAHELLSLLEFPLAAPSANPSGYISPVTAEHVAQGLGGKIAYI
                     LDGGTCTVGLESTVVTFEEDKIIVLRAGGVTVEALREFGPVEVRTHAAASTGSPGLLK
                     SHYAPRTRLVTGNLRTLLDEHATKKLAVLSLWELMRGENIVASEVLSPDSDLTEAARN
                     LFAAMRRLDAVGADIIIAERMPEHGFGLAINDRLDRAGA"
     gene            complement(5089..5415)
                     /locus_tag="PLF85_05360"
     CDS             complement(5089..5415)
                     /locus_tag="PLF85_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNE19122.1"
                     /translation="MVFATVYPLYMAKVERKGRSKAELDAVIAWLTGLNAKKLQTLLS
                     QKVTFSNLFSQAKLHENAKLIKGTICGVRVEEIKNPLTQKVRYLDKLVDELAQGKSLE
                     KILRQQ"
     gene            complement(5548..5859)
                     /locus_tag="PLF85_05365"
                     /pseudo
     CDS             complement(5548..5859)
                     /locus_tag="PLF85_05365"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
     gene            complement(5870..6295)
                     /locus_tag="PLF85_05370"
     CDS             complement(5870..6295)
                     /locus_tag="PLF85_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19123.1"
                     /translation="MLRIGLVLLLGATTIAAEIHPMYYAGQQQSAPEFLAIAVEGVEP
                     KTLPKEEYVATRVRAKVERIFRSKSRIKKGATVEIRYTIFQPREGFTGPRPMPYLKSG
                     ERHVFYGRVIERNKEGVWILEPVAGGRSFETVTKEEANL"
     gene            6531..7760
                     /locus_tag="PLF85_05375"
     CDS             6531..7760
                     /locus_tag="PLF85_05375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNE19124.1"
                     /translation="MHKVVILGAGTGGSIMANRLRKQAKKGELSLTVIDPAAQHLYQP
                     GLLFLPFGQTTEKKILRPNRNFFHRSVNLVQQPVQSLDAAARAVTLGDGSVINYDILV
                     IATGTKTAPDETEGLTGEGWYTNRFDFYTLEGSRRLAEALKKFTGGHLVINIVDMPIK
                     CPVAPLEFAFLADAYFKKCGIRDKVRISYVTPLDAAFTKPVAAKVFGDFLAQKGIELV
                     AEFNTGRVEGNQLVSWDERTVGFDLLVTVPLHSGADFLKGTEIADDLGFVCVHKGTLQ
                     SEKYPEIFALGDAANVPTSKAGAVAHFESESLSDNILRYIRKEALQADFDGHANCFIE
                     TGHGKATLIDFSYDTEPLPGKFPYAGIGPLKLLKETRMNHWGKLAFRFIYWYILLPAR
                     WLPIGRHFTMKGKFRPS"
     gene            7806..8123
                     /locus_tag="PLF85_05380"
     CDS             7806..8123
                     /locus_tag="PLF85_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HNE19125.1"
                     /translation="MATKTFGNKTVEVDEQGYLKNTADWSEELAKALAADVGISEMTE
                     GHWKVVKFMRERFLKNGDSPSIRTLGKDSGVTTKELYQLFPKGPAKLAAYVAGIPKPR
                     GCI"
     gene            8152..8619
                     /locus_tag="PLF85_05385"
     CDS             8152..8619
                     /locus_tag="PLF85_05385"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNE19126.1"
                     /translation="MENTIKKVSVIISKGSLEGIYPGLIMANGARMEGIEANLFFTFF
                     GLDAIHKKKQHRIKVATVGNPGLHIPTWLGGIPGMSAMVTWRMKKSIESLDIPPVGEF
                     LQLIHDSGCGMYACKATVDMFGMTKADFVSPVDDIITVGEFYERAQGGEIIFT"
     gene            8650..9537
                     /locus_tag="PLF85_05390"
     CDS             8650..9537
                     /locus_tag="PLF85_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19127.1"
                     /translation="MNRLDISFASGEKNQLCRGWLYLPEKKGARFAAVVMAHGFGAVK
                     ELRLDAYAEKFAAAGFAVLVFDYRHFGQSDGEPRQLIDISQQLEDWRAAINFVRHHSS
                     IDAGRIALWGTSFAGGHVVEVAAQDPQIRCVISQVPHLDGFASASAAGGAQAARLAME
                     AARDAASGLIGLEPHYVKIAGAPGELAAMSSENVATIWPRMVPEGFSFDNRVAARIFL
                     NLPLYSPERKAEQLTMPWLVQIATRDQTTPVEPAERAAKKAPLATLTRYEADHFDVYL
                     PPLFDTVVKEQLEFLQRHL"
     gene            complement(9587..10756)
                     /locus_tag="PLF85_05395"
     CDS             complement(9587..10756)
                     /locus_tag="PLF85_05395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNE19128.1"
                     /translation="MPKISLLSGRDKPVRFGHPWVFSGAIQPATLGAGLVTVLAHNKE
                     VLGTAFYNPGCRLALRMLSRGGAPPFTEHEIPVRLRSAIARRSHLQDSNTDAVRLVFA
                     ESDFLPGLIVDRFGDCLSVQFNSAFAEHFRAEIVAALGVLLNPHFLVDTSDNQARNRE
                     NLPPVAAAAGPAMEIRSRGVRYRVAPGSGQKTGFYLDQATNREIVAAHARGRRVLDAF
                     CYSGGFGISCLKAGAHEVTAIDSSADALQLLMQNQELNRENLPANATIKTIQADVFEE
                     LRRLKAAGEKFDLIILDPPKLAPSRSKTDAAEKAYKDLNLQGLRLAAPGALFATFSCS
                     GAMTFEKLRQVLHYAAHDAGKEVQILQALQQGEDHPVMLAFPESEYLRGYLCRVF"
     gene            complement(10842..>11368)
                     /locus_tag="PLF85_05400"
     CDS             complement(10842..>11368)
                     /locus_tag="PLF85_05400"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE19129.1"
                     /translation="NPGAGATLSALTIFVTASTHAANLNVAGPDNICRQDLANPKGLG
                     NGVWRALLGYNTRRACSTANCGGGAAENLNWALRPKQNYARPDGAYIGRTDDSSLFVL
                     PLAKSFSAVPGLKAWSGLDTNWLNSLVCSDWITTSGATSGMRAAADAVTIAAISEAAE
                     ICSSSLALICVEQP"
BASE COUNT         2396 a         3122 c         3137 g         2713 t
ORIGIN      
        1 ttagagggac aaaacgacca attatgtgaa gtaagccctt aaagggctat attttatacc
       61 ccagcaacag ctgcacccgc ggaaaaaaca gcggttggtt gatcgcggcg tcggcgtcgc
      121 tgtagcggta gttattgcgg gggttgtcgt cctggttgtt ggcgttgtag aagaagccgt
      181 ggcctttcag ttgcgtgttg aagaaatatt cgacagtggt ttcgaggccg atcacaaaat
      241 ggtcagaaat cgtgagcaga gatttaaatc ccacaccaaa accccatgcg ttggaataga
      301 ccttgaacgc ttcattgccg ccctggtagt cgaagaaggc cgcatagctc gagtagcgcg
      361 gccctgcggc gacattgagt tcaaagattg attgccgcac aatcgggtag agcgcgtcga
      421 cgcccagaaa gaaggtcgca cccgattcga caatgttatc gttgccacct gtcgcgtcgt
      481 tgatgaagat cttgcgcgca tcgaccgcgt tgcccgggtc tgactggaaa acataaccgg
      541 cggaaaaatc aaaaccgatc ggaaactttg gcgcgatgcg gtgcaggccg aaccccgcat
      601 tgaggccaaa gccgttcatg gtgcccgcct cggcgtagaa gattttttcg ggggcagcgt
      661 tcacaggtag gccaaaaaga gcgcagcaga gcagggctaa ggtcaggttt cggacgcgca
      721 tagttcaggg gttgagttgg ggcagcgttg tcaattcagt agccggtcat ccagcaaaat
      781 ccggatgaaa tggatttatg acgacgagtc ttccgtattt ttggccatga ttgagatctt
      841 ccgttagcag atattcgctc tgcgaacgtt gtgcagcagc gattatcagc gaatcccaaa
      901 acgataaggc atagcgcctt tgaacttgaa acgcttcttc gatgagtttg ataccggccg
      961 gtattacatt ccatacgaaa taatctttca caatcttctg agcttcgtcg gcctcgaccc
     1021 tgtgtctgat cagagtgacg tagaattcct gcagaacctg ggtgctgatg ctcggaagcg
     1081 gctctttttg ccacaagtat tccaacaagg cgcccgctcg tgcatatttt tccgggtttc
     1141 caatattaat gccgtagaat aggatatttg tgtcgatgaa tatgtctgcc gccaggatat
     1201 cccggagtcg atcattcata aacactttct cgcgaaaacg cgcctttgcc cagactgacg
     1261 cctttccgga tagttttaag agcatgtgac ctggctttgt ctatttctgg tgcatggtca
     1321 gtacttttct caatcagctc tgtaacccat ttcgaaagcg acaagccttg ctcaaccgca
     1381 atatgcttcc cttttttcag cgtgtcttca tcaatacgga gcgtaatatt cctgagcatc
     1441 cgttacgctc gtccgcccta atctcctgcg tcaacaaatt aacacaggaa cacagttttt
     1501 tagagcatgt gcctcatcgc ccgctgcagc acgtcatctg catattcatc gcaatccgcc
     1561 tccccctccc tcggcgccca tggggcgaag tccttgtcgt tcgccggggc gtacgggccg
     1621 ggtttgactt cgaagcagat tgcggtgtcg ctctccacaa ttatgctgtg ccagactccc
     1681 ggggcgatgt cgatgccatg gttgtcttgg gccgaaagac gcgtcacagt ttgaagctcc
     1741 cccacgtcat caaacgtgat aaagagcagc tgcccgcgca gtaccacgaa actttcgctc
     1801 ttgggtggtg tggtgtgacg gtgcggagtg acatacgtgc ccttcaccat cacgttgaga
     1861 aaacgattgg ggttctcttc aaaactctgg tgaaagttgt ggttggttcg ctgccgttct
     1921 gatgccttcg cgcgctcggt caggctgtcg agaagcgcgg gagtgatgat ttgaaccggt
     1981 tctgccattg ctgttacgcc actgccagtc gcttctgtaa tgcctcgagt ccgggcgtct
     2041 tgatatccag ttctgccccg gccttgatcc aggcagaaag catgtcgagt gactggtcgc
     2101 gcaccttcgt gtagtgccgt tcaaagaaac gctccacatc aagcggtgct atcttctgcg
     2161 ttacctgcat catgccgacc agcgcccgtc cgggaatcag ggtgcgttca tatggtactc
     2221 gtgcattgtt cttgcgtgcg ccgatgaaag aagcctcgac cattgctttg cgtgtgatcc
     2281 caagcagttc tttgtccgct gcgagttttt tgaggctcca gcccgaacac tcgagcgcat
     2341 agattgacgt ctgcagcagg gcagacccaa gcgcgccttc gagctgcacg ttgttgtgtt
     2401 ttgccgaagg catgccgccc ttgttgagca ggtcaacgat tgcctgcgtg cgctgcgcct
     2461 cgccgctgta cgtgaatttc agcagcgggg gaaaccagta ggcagtcccc gggcgcgcga
     2521 cctcttcacc cggcaggggc gccgagtagc tcatcatggt aatgagaccc gtaatgatat
     2581 tctgctcgtt cgtatagcgt ttcacgaacg ccaggtcttc gataccgaca gtcagaatga
     2641 cgacattcgc gttaccgata gcctttgcca gttcagcata ccaatcaccg gcccgcaggg
     2701 ccgttgatga tgttgtgaga aagacatagt cccactgttg ttttgccacg tccgccatat
     2761 cggtgagaag gctgaacttg tcgaataaca cggggttctg ccgtacatcc ttcccgttga
     2821 gcggatagag cgtgaagcca tctcttgccg ctgcggcgtg cctctcttta atgaggaaac
     2881 ttgcatcagc gccagcccgt accaggtggt acccatagac ctgtcccacc gcgcctgcac
     2941 ctacaatcag atatttcatt ttttgaatcc tcatgccaga ttggaacgaa gaccagcaaa
     3001 cgcatctctt tattagctgg caaaccgctc gagcatgcgc tctatctggc tagcttcgaa
     3061 cggcttgccg atgaactcgt tcatacctgc ctcgcgcgct aacgcctgat cttcagccat
     3121 cgtgttcgca gtcacagcta tgacgggcag tgtcgtggaa accgtctcgc gaatcctttt
     3181 cgtgcactcg ataccgtcca tcaccggcat ctggatatcc atgaagacca cgttgaagtc
     3241 tttgaacttc gcaagctgct cgagcgcttc ctggccatgg ttggctactg ttaccttgca
     3301 gccttttccc tcgagcgtct tttgcagaat aatctggtta atcaggttgt cctcgacaat
     3361 caggcaatgc gtgcgcggcg gagctttttc ggttgactgg cgctgcacga gatggcggtc
     3421 aaagtcgaac ctgccgagaa attcttcctt ggatgaaaac agaaactttt ctgcggtctg
     3481 gtcgatgatc tggtcgacga gcgagatggc ctcggggctc aggcgcccgt tctggtcggc
     3541 ctcgagcgac aggtactgca ggttggccat caggttacca atcgaatcct gcagcatgcg
     3601 catcacgttg atcagcccat ggatgcggtc attgtcgagg ttctgtgctt tgcgcaatat
     3661 ttcaaagtgc tgctgcgtct gcacgagcat cgtgatgtca ttctgtacgc cgacaaagta
     3721 gttcttgggc ccgacactga tcgggtagat cgaaagccgg ttccaaaact tctgcccgtc
     3781 tttcttgtaa ttgagcaacg tgacgacgca ggcctcgcgt tcgttgagct tcttgcgcag
     3841 gtactcaagg ctgcccggat cagtctcggt tccctgtaag aagttgcagt tttttccaat
     3901 aatctcagtc gccgggaacc cagtcatttt ttcaaaaccc tggttggcat aaataattgg
     3961 tctgccggga atatcggcga caatgatgaa gccttcctgg tgtgacccga cggcaatgct
     4021 gagtacttct tttatgtttt ccatggccgt ttattttatg cccgcaactc tgtaacaggc
     4081 gcgcgcgtgc cagcgtgatt accctacctg cagcggggag caatttcccc tggcaacctg
     4141 ctacgccccg gcccggtcca atctgtcatt gatcgcgagc ccaaaaccgt gctcaggcat
     4201 gcgctcggcg atgatgatgt cagcgccgac tgcatctaac cggcgcatcg cggcaaacag
     4261 gttgcgcgcg gcttcagtca ggtcagagtc aggtgaaaga acttcgctcg cgacaatgtt
     4321 ctcgccgcgc atgagctccc acagcgaaag cactgccaac tttttcgttg catgttcatc
     4381 gagtaacgtt ctcagattgc ctgtgacgag gcgtgttcgg ggcgcgtagt gggatttcag
     4441 aagtcctggt gatcctgtac ttgcggcagc atgagtgcgt acttcgaccg ggccaaactc
     4501 gcgcagcgct tcgacggtga cgccgccggc gcgcagcacg atgatcttgt cttcttcgaa
     4561 agttaccacc gtcgattcga ggccaacggt gcaggtaccc ccgtcgagaa tgtaagcgat
     4621 cttgccgccc agcccctgcg ccacatgctc tgccgtgaca gggctaatgt aacccgaggg
     4681 gttggcgctc ggcgccgcga gcggaaactc gagtaacgat agcagctcgt gcgccatcgc
     4741 gtgcgcggga atgcgcagcg cgactgtatc gccgccacca gtcgtgacat cgggtacaat
     4801 gggtttcttc ggtagtacgt acgtcagcgg gccaggagaa aaccgctcgg cgaggcggta
     4861 ggcgagcggg ggcacctcgg cgtaggtcgc catcgcctct ttgttcgcca ggtgtacgat
     4921 cagcgggttg aactgcgggc gccctttggt ggcataaatc ttgagtacag catcggcatc
     4981 gagcgcattg cctgccagac cgtagaccgt ctccgtgggt atcgcgacca gctcgccgcg
     5041 gcgcagcagc gcagctgcga agagtatatc agtacctgtt tcggtcattt actgttggcg
     5101 cagaattttt tcgagcgatt taccctgcgc gagttcgtcg accagcttgt cgaggtagcg
     5161 cactttttgt gtcagcgggt ttttgatctc ttcgacgcgg acaccgcaga tcgttccttt
     5221 gatcagcttt gcattttcgt ggagttttgc ctggctaaac aggttgctga atgtcacttt
     5281 ttggctgagg agcgtttgca gctttttggc attgagacca gtcagccatg caatgaccgc
     5341 atcgagttct gctttgctgc gccccttgcg ctcaactttg gccatgtaga gggggtagac
     5401 agtcgcaaac accatgttgg ctatccgctc gtccgggcta ctcggctttc tcgctgttgc
     5461 agtcgagggg gacagtttga ccagcttacc cttacgcttg acgatgttct tgtagtccca
     5521 ttgtatttcg cgggattttt tgagccactg cttcaggtct tttgcagata cttccctggc
     5581 agaggtatag agtatagaag cgtctttaaa ttttccgctg cccggtacca gtttgtcttc
     5641 gccaaaatct gccccgctcc agaacatcag gcgtatgcca gctttgattt tgctgtaacc
     5701 gacaatcggg ttgccttcga gaaaccacac ggggtggccg tgccagatct tgttttcaga
     5761 tcctttgagc ccggcatcaa tcgtcttcgc cagcaaatcg cagatggctc gctctgcagc
     5821 agattgtgtg ctattgtatt tttttatctc ggcttgcata tggtagatcc taaagattag
     5881 cttcttcttt cgttactgtt tcaaaactgc gcccgccagc aacaggttcc agaatccaca
     5941 ctccctcttt attcctttca atgacgcggc cgtagaaaac atggcgttcg cctgatttga
     6001 ggtagggcat cggccgcggg ccggtaaatc cttcacgtgg ctggaagatg gtgtagcgaa
     6061 tttcgaccgt cgctcccttc ttaatgcgcg acttgctgcg aaatatacgt tcgactttgg
     6121 cgcgcacacg cgtcgcaaca tattcttctt ttgggagcgt cttgggttct acgccttcta
     6181 ccgcgatggc caaaaattcc ggggcagatt gctgctggcc ggcgtagtac atcgggtgaa
     6241 tttcggcagc tatggtcgtt gcacccagca gaagcaccaa accaatccgc agcatgcatt
     6301 ctcgacctgt ttgctcaatg cgtcgtcaag gttgattgtt agcctcaccc cccggccccc
     6361 tctccattat ggagaggggg tgaaagagta ttttgccgtt ccgaagcaaa cctatcttgc
     6421 tcccccttct ccacgttgtg gagaaggggg tcggggggat gaggccagtt aattatggac
     6481 aattcagtcg tttttgattg ccatacctgc ccgggttcgt gtgatgccct atgcacaagg
     6541 ttgtcattct cggcgcgggc actggcggca gtatcatggc caaccgactg cgcaagcagg
     6601 caaaaaaggg agaactctcc ctcacggtga ttgatcctgc tgcgcagcat ctctatcaac
     6661 cggggctgct gtttttgcct ttcggccaga caacagaaaa gaagatactc cgccccaacc
     6721 gcaatttttt tcatcgcagc gtaaaccttg tccagcagcc ggtgcagtcg ctcgatgcag
     6781 ctgcgcgcgc cgttaccctc ggtgacggtt ctgtgattaa ttatgacatt cttgttatcg
     6841 cgacaggtac aaaaaccgcg cccgacgaga ccgaggggtt aactggcgaa ggctggtaca
     6901 ccaaccgttt tgatttttat acgctcgaag gatcgcgccg gctagccgaa gcgctcaaaa
     6961 aatttaccgg tggccacctg gtgatcaaca tcgttgatat gccgataaag tgtcccgtgg
     7021 cgccgcttga gttcgccttt ctcgctgacg cctacttcaa gaagtgtggc attcgcgaca
     7081 aagtgcggat ttcgtacgtg acacccctcg acgcagcgtt caccaaaccg gtcgccgcca
     7141 aagtcttcgg cgactttctc gcacagaagg gtattgaact cgttgccgaa tttaacactg
     7201 gccgcgttga gggtaaccag ctcgtctcct gggatgaacg cacggtcggt ttcgatctgc
     7261 tcgtcacggt accgctgcac agtggggctg attttttgaa gggtactgag attgccgacg
     7321 atctgggctt cgtgtgcgtt cacaagggaa ccctgcaaag cgaaaagtac cccgaaattt
     7381 tcgccctcgg tgatgcggcg aacgtgccga cctcgaaggc aggggctgtc gcacatttcg
     7441 aatcagaatc gctttccgat aatattctcc gttatatcag gaaagaagcc cttcaggccg
     7501 actttgacgg ccatgcaaac tgctttattg agaccgggca cggcaaggcg acgctgatcg
     7561 atttcagcta cgacaccgag ccgctgcctg gtaagtttcc gtatgcgggt attggaccac
     7621 tgaagctgct gaaagagacg cgcatgaacc actggggcaa gctggccttc aggttcattt
     7681 actggtacat tctgctgccg gcgcgctggc tgccgattgg ccgccatttt accatgaaag
     7741 gaaagtttag gccatcctga caggcaggca aagcctgcct gtcaggatgg ctttttagga
     7801 ggattatggc aacgaagact tttggcaata agaccgtcga ggtagacgaa cagggctacc
     7861 tcaagaatac cgctgactgg agcgaagagc tggcgaaagc cctcgcggca gatgtcggca
     7921 tcagcgaaat gacagaaggt cactggaagg tggtcaagtt catgcgcgag cgtttcctca
     7981 agaacgggga ctctccgtcg attcgcacgc tcggcaaaga ctcgggcgtc acgaccaaag
     8041 aactgtacca gctctttccc aagggcccgg cgaagcttgc cgcctacgtg gccggcattc
     8101 caaaaccgcg cggttgcatc tgatcgcgca atgcactgaa aggagaagca aatggaaaat
     8161 accatcaaga aagtctcagt catcatttca aaaggatcgc tcgagggcat ctatcccgga
     8221 ttgatcatgg ccaacggcgc gcgcatggaa ggtatcgaag ctaatctctt ctttactttc
     8281 tttggccttg atgcgatcca caaaaagaag caacaccgta ttaaggtagc aacggtgggc
     8341 aatcccggcc tgcacatacc cacctggctc ggcggaattc ccggcatgtc ggcgatggtg
     8401 acctggcgca tgaagaagtc gattgaatcg ctcgatattc cacccgtggg tgaattcttg
     8461 cagctgatcc atgattcggg ctgcggcatg tacgcctgca aggcgacagt cgacatgttc
     8521 ggtatgacga aagccgactt cgtctcgccg gtcgacgaca tcatcaccgt gggcgagttc
     8581 tacgaaagag cgcagggcgg tgaaatcatt tttacgtgac aggttgcttt cagcaacatg
     8641 tcacgtaaaa tgaatcgtct cgatatctcg ttcgcctcgg gcgaaaaaaa ccaactctgc
     8701 cgcggctggc tctacctgcc tgaaaagaaa ggcgcccgtt ttgcggcggt tgtcatggcg
     8761 catggctttg gcgccgtcaa agagcttcgg ctcgatgcct acgccgagaa gttcgcggca
     8821 gcaggttttg cggtacttgt cttcgactac cggcattttg gccagagcga cggcgaaccc
     8881 cgccagctga tcgacatcag ccagcagctt gaagactggc gcgcggcaat taactttgtg
     8941 cgccatcata gctccatcga tgctggccgc attgcccttt ggggtacgtc ttttgccggt
     9001 ggccacgtgg ttgaagtcgc cgcgcaggat ccacagatac gttgtgtcat ttcgcaggtg
     9061 ccacacctcg acggttttgc ttcggcatcg gcagcgggtg gtgcgcaggc agcgcgtctc
     9121 gccatggagg cagcacgcga tgcagccagt ggcctcattg gtcttgagcc acactacgtg
     9181 aagattgcag gcgcgcctgg tgaactggca gccatgagct cagaaaacgt tgccaccatc
     9241 tggccacgca tggtgcccga gggattttct ttcgataacc gggtcgctgc ccgcatcttt
     9301 ctcaacctgc cgctgtattc acccgagcgt aaggctgaac aattgaccat gccatggtta
     9361 gttcagattg cgacgcgcga ccagacgaca cccgtcgagc cggcagagcg ggctgccaaa
     9421 aaggccccgc tcgcaactct cacccggtac gaggccgacc actttgacgt ctacctgcca
     9481 ccactctttg atactgtagt caaagagcag ctggagtttc tgcagcggca tttgtagtcg
     9541 ctaccagaca acccggactt cttgttccgc tgcagtatag ctgcgtctag aaaaccctgc
     9601 acaagtagcc gcgcaggtat tcgctctcgg ggaatgcgag cattaccggg tggtcttcgc
     9661 cctgctgcag cgcctgcaga atctgcactt ctttgcctgc gtcgtgcgcg gcatagtgca
     9721 gcacctggcg cagcttttca aaagtcatcg cacctgagca agaaaacgtc gcgaacaggg
     9781 cgcccggggc tgcaaggcgt aaaccctgca ggttgaggtc tttataagcc ttctctgctg
     9841 catcggtttt gctgcgcgag ggggccagct tgggcgggtc aagaataatg agatcaaatt
     9901 tctcgccagc agcttttaac cggcgcagtt cttcaaacac atcggcttga atcgtcttga
     9961 tcgtcgcatt ggcgggcagg ttctcgcggt taagttcctg attctgcatt agcagctgca
    10021 gtgcatcagc agaactgtcg atcgcagtca cctcgtgcgc accggctttg aggcaggaaa
    10081 tgccaaatcc accgctgtag cagaaggcat cgagtacgcg ccgaccgcgt gcgtgcgcgg
    10141 cgacgatttc gcggtttgtc gcctggtcga ggtagaaccc tgttttctgc ccgctgcccg
    10201 gtgcaacccg gtaacgcacg ccgcggctgc ggatttccat agcggggccg gcagcggctg
    10261 caaccggtgg caggttttcg cggttgcgtg cctggttatc agatgtatcg acgagaaaat
    10321 gtgggtttag caatacaccc agtgcggcga caatttctgc ccgaaagtgt tctgcgaatg
    10381 cgctgttgaa ttgtactgag aggcaatcac caaagcgatc gacaataagc ccgggcaaaa
    10441 agtcgctttc ggcaaaaacc aaccgcactg catcggtgtt gctgtcctgc aggtggctgc
    10501 gccgggcaat ggcggatcgc agtcgcaccg gaatttcgtg ttccgtaaac gggggagctc
    10561 cccctctgct gagcatgcgc agcgcgaggc ggcaacctgg gttgtagaaa gctgtaccga
    10621 gtacttcctt gttgtgcgcg agtacggtta ccagcccggc gccgagcgtt gcaggctgta
    10681 tcgcccccga gaaaacccac gggtgaccga agcggacggg cttgtcgcgg ccggacaata
    10741 gggaaatctt cggcaaactc atatcgcgcc cattagacca tagcgcggcg ttgagcggcc
    10801 gctgcaagca ggctcgcaaa acgtttcagt aggttgctaa attacggttg ctcaacgcag
    10861 ataagtgcga gacttgatga acatatttcg gctgcttcag aaatggccgc gatcgtgact
    10921 gcatcggctg cagctcgcat tccgctggtt gcgccacttg tggttatcca gtccgagcaa
    10981 acgagcgagt tcaaccagtt tgtgtccaaa ccagaccagg ccttgaggcc aggcacggct
    11041 gaaaagctct tcgcaagggg cagaacaaag agggaagagt catctgtgcg gcctatgtat
    11101 gcgccgtcag gccttgcata gttttgcttt ggtcgcaacg cccagttcaa attctccgcc
    11161 gcacctccac cgcagttggc agtgctgcaa gcgcggcgag tgttataacc caaaagcgcc
    11221 ctccaaactc cattgcccaa acctttcggg tttgccaaat cttgccggca aatgttatcc
    11281 ggacccgcta cgttgagatt tgctgcatgc gtcgaagctg tgacgaatat cgtaagcgcg
    11341 gaaagagtgg cgcctgcccc gggattct
//
LOCUS       DAOQJF010000069        11203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_2951_length_11203_cov_5.094367, whole genome shotgun sequence.
ACCESSION   DAOQJF010000069 DAOQJF010000000
VERSION     DAOQJF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2951_length_11203_cov_5.094367"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PLF85_05405"
     CDS             <1..866
                     /locus_tag="PLF85_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE19130.1"
                     /translation="AQGKIRSCFAMTEVDTAGSNPTLLEARAVKDGDHYVLNGRKWYT
                     TAAEGSAFAIAMMVTNPDVNPAMRASMFIVPTNTPGFRILRNIPIMGEAGSDYASHAE
                     VVFENCRIPKENLLGREGQGFKLAQERLGPGRIHHCMRWLGISARAFEMLCRRANQRK
                     IHNDPSTGSGRVAGIYLADSEITQLYVAELAAELHGARLMTLSAAWAIEHLGMSKARD
                     EVAMIKFVVANVMNKILDRSLQIHGGLGMSDDTILAYWFRHERAARIYDGADEVHKAS
                     LGKRILRDYRR"
     gene            complement(863..1564)
                     /locus_tag="PLF85_05410"
     CDS             complement(863..1564)
                     /locus_tag="PLF85_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19131.1"
                     /translation="MLADQHDQTAGSVCLQDSRLEVTTHDASHATEDLLRNYARKLTS
                     RFNRPDLSPALEIILKELTTNAAKANFKKLFFAENGLLLEDPEQYQKGVLMFRQIFSE
                     DMFASYSHKAREARLVVTTTIDFNDDRFIIEIRNNVPMSSAEERRVREKLRLAMGCTH
                     AEHFIMENIDETEGAGLGLMLCIAALRSVSVNPRYLSIATDQKSHTVARLELPLHSGY
                     APQRARFAQATHAVS"
     gene            complement(1751..2461)
                     /locus_tag="PLF85_05415"
     CDS             complement(1751..2461)
                     /locus_tag="PLF85_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19132.1"
                     /translation="MTRIWAPGADEEAADEIIENENRLVVRAYGLTRSTEDFLRSYLR
                     RVLQETGRPELSATLEIILKELTTNAAKANFKKIFFAENQLRIDDPEHYEQGVVRFRE
                     TFSEEIFIEYRQKAQASELLVLISVDYDPDRVILEIRNNVPMTPEEEKRAREKLRPAM
                     TYEDMGQFIMDNADETEGAGLGIMLCLTTMRSSSIDPRLFSISTDFRTHTLARVEVPL
                     HEGYTPSRARYAQRRRSA"
     gene            2674..3420
                     /locus_tag="PLF85_05420"
     CDS             2674..3420
                     /locus_tag="PLF85_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE19133.1"
                     /translation="MQIKDNTFLVTGGSSGLGLATVQRLAEHGANVVVADLAPPADLK
                     HSGAEFIKTSVTNPDEIQAAVDLAFSRFGSLRGVINCAGIGNVVKTVSKKKFGALDAA
                     RQIVEINLLGTYNVISIASTPMSKQDPLADGERGVFINTASVAAFDGKVGQAPYSASK
                     GGVASMTLPLAREMAQYGFRIMAIAPGAFETPMTARLPGKATEELVDTIPFPNRMGQP
                     AEFAQLVQSIIENTYLNGEVIRLDGGIRMT"
     gene            3417..4151
                     /locus_tag="PLF85_05425"
     CDS             3417..4151
                     /locus_tag="PLF85_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19134.1"
                     /translation="MKALPKLKVHWGIAVAMLLVSGLPAFWFTKFFLRDAYQLYRAQS
                     WPIREVVIQTAAEDTLPEPGQGHGPGALFERIEYRFESDGKQYLSRRYGPFQRWNFGK
                     NAPRFTEGAAVRAHVNPADLYESVLDNRVSEGFWTVLWLTVLTVAWLVWALLTVLRSR
                     RFFRAFLGHLLLLLSAGILAAALVASEVERTRDWWFAVGGFLSTIALVGLSVILSLWN
                     RRAYLLAGIGIVAGFVSIFVGVAIRE"
     gene            4167..4733
                     /locus_tag="PLF85_05430"
     CDS             4167..4733
                     /locus_tag="PLF85_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19135.1"
                     /translation="MKNLPPYTMRYPVAGLLEIRVRPRIFFPLLVLGIMLPLFLFRLF
                     FSVEFKAFWRMALRVFGTDFANEEAEDIYNTFPVFSALSFLLLGSWLVLHKRIGLIQV
                     TREGVRIFRNPHFLPRSQVWIPRAEIKSLRDEKLASRNDDIGYVLQLETHSLGNFELV
                     RIFGSGNDDRQRFGQLGNEIRRVLGGIG"
     gene            4779..5015
                     /locus_tag="PLF85_05435"
     CDS             4779..5015
                     /locus_tag="PLF85_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19136.1"
                     /translation="MQAEQVAALNKIMSIIDQKASEYKQEYMRLPQSKRFANRKLILD
                     LIDDATELGNAMKPAPRDALEDLKRLAEQLNRLA"
     gene            5017..5901
                     /locus_tag="PLF85_05440"
     CDS             5017..5901
                     /locus_tag="PLF85_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19137.1"
                     /translation="MQRFALFLLAVLLAGAATMLGVVSAFERTAANPEYYSNAINQIG
                     LTGFAVPLAEKLTGQNGKVAQVLQLGMNRLEPQLKQQTAAHLQQFFQYLRGKTEKFDL
                     VYDMTTLQSDTAFTGEVVETLQKDSKLGLLPRSLLQPVAQKLTNQLPGKIDLLSLAGR
                     DEGSMASLRKDAQAWLSTADRWLIISAALCLLFVVLIFLAARSIPRTLTTVAVGLLVA
                     AAVLMLPWVFSDAILAMLRLKGAIYEFLQQTGLWQALAAELSKEYLVSPVTAAATGVV
                     FLVGGISLRRFVPYSGKK"
     gene            5913..6440
                     /locus_tag="PLF85_05445"
     CDS             5913..6440
                     /locus_tag="PLF85_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19138.1"
                     /translation="MARVLKLPLLLTLLSLWPALQATQPRAGVAFSPGYLTAQGTVAY
                     AASAEWEFSLGARIALRSDVHALLARPQDSNLTQNYQGFVGLLYYFDQWFGSQPFAGF
                     QPGFGVGKVNTPANPGFRLYPAMSPVFGLHLPLLGTLQLTFALRYVFGELHYRDTGAV
                     YLSELRIALGLGANL"
     gene            6530..7249
                     /locus_tag="PLF85_05450"
     CDS             6530..7249
                     /locus_tag="PLF85_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19139.1"
                     /translation="MSAGSTLAFRLTIFLFGTALAPISILGLAAIRLSEKVGSGSDAP
                     VRIVLLIALMLVLGIYLSLLLARTLRTPLRELQRAAAQIRGGDYHVRLPVTTNDEVGR
                     LTQGINEMAAGLREREHIRETFGRAVACALDMLATLERLNAELAAEKIPEVRIGIGLH
                     CGPVTAGSVGSNDRLEYTVIGDTVNTASRIEGLCKKAGRPLLLSSAVAQACGDEWEFV
                     RLGKARVKGKTDALEILTVKS"
     gene            7387..8202
                     /locus_tag="PLF85_05455"
     CDS             7387..8202
                     /locus_tag="PLF85_05455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE19140.1"
                     /translation="MTVRKNKETFAEPVPAHWAGKDALQSHVLNVLSLMFPDGEAFFI
                     RSVKHFADRVTDQKMQADIKAFVGQEMQHSMAHERFNKAVAENVGNMDWFMWLFTVPT
                     FEWLEPVVRKNLGFEILHKWALAITAAAENMTAGFAQSLFQPGELEKIARPDVRELYA
                     WHAAEEMEHRDLAYDLYQQVGGDYATRMAGMAFAYGIIAAYVAVGTTYLVLNDKQFPW
                     ATLPQQLVDLVTKENSLGRVFLEGMVDYIRPDFHPSQHPMNAEAVAYLEKMAS"
     gene            complement(8212..9654)
                     /locus_tag="PLF85_05460"
     CDS             complement(8212..9654)
                     /locus_tag="PLF85_05460"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNE19141.1"
                     /translation="MAKSTATPAADTSKRVHAFTDDAMATHDAVALAELIRKGELDAT
                     EVAKAAIARARSVADKLNAIEIAAYDQALAQTHLPAKPGVFAGVPTFVKDNADLEGFP
                     TRNGTRSFTPAAAEENGPYTEQYLSQGFTVLGKSQMPEFGFNASTEFEGDHPTRNPWN
                     TDYSPGGSSGGSAALVAAGVVPIAHANDGGGSIRVPAACCGLVGLKPTRNRHLNNKSA
                     QSMPINIVSEGVVTRSVRDTAQFMYGMESVYKNPRLKPVGKVEGPSQKKLRIGLVIDS
                     ITGTPTCPETRAAVEDIARLMEKLGHRVEPITLNLPATFIDDFTLYWAMLAFMMGSLG
                     KFLLGSDFDYDRLDPFSKGLVQHYRKNLLHTPAALWRLHRSANLYAEIMKPYDAVLSP
                     VLGHTVPKLGHLNPGQGYDDLMEKLSRYIMFTPLNNATGSPAISLPLAHSAEGLPIGI
                     QFQAAHGNERTLLEIAYLLEAERPFNKIQA"
     gene            complement(9705..10394)
                     /locus_tag="PLF85_05465"
     CDS             complement(9705..10394)
                     /locus_tag="PLF85_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE19142.1"
                     /translation="MKVILVTGSNRGIGKETIRQLARVGHKTILTGRNADHVRDAQDD
                     LAAEGVITDAFACDVRDEAQVRKLVQYVDERYGKLDVLVNNAGIFLVSSDSTKADIDI
                     IRQTFDTNVLGPYRMIEVLLPLLRKSGDARIINLSSGMGGLTEMDGGYPGYRISKTAL
                     NAVTRIFANDLAKDKISVNSVCPGWVKTDMGGERATREVEQGAETIVWLATTEKPPTG
                     KFLRDKKEIPW"
     gene            10573..>11203
                     /locus_tag="PLF85_05470"
     CDS             10573..>11203
                     /locus_tag="PLF85_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE19143.1"
                     /translation="MSTRALILSGGGARGAYQAGVYRYLSEINWKPDLISGTSVGAIN
                     ACAIGSGFDAQKLQTFWLNIAQDHVFAFSFWRKIWHFISRRGYSAVLDTTPLRKFLSN
                     NIDIRALTESDIEVVICAINIVRSKLRFFTNKEIGVEEIMASSAIPLLFPWQEIDGEP
                     HWDGGIMANTPILPALERGSREIVVVLLSPVGGMSMAIPKSRKKIAELIL"
BASE COUNT         2257 a         3064 c         3283 g         2599 t
ORIGIN      
        1 tggcacaggg gaagatccgc agctgttttg cgatgaccga agtcgacacg gccggttcta
       61 acccgacgct gctcgaggcg cgcgcggtga aagatggcga tcactatgtt ctcaatggcc
      121 gcaagtggta caccaccgcc gccgagggtt cggcgtttgc gatcgcgatg atggtcacaa
      181 accccgacgt taaccccgcg atgcgcgcga gcatgttcat cgtgcccacg aacacaccgg
      241 gttttcgaat cttacgcaac ataccgatca tgggcgaagc aggcagcgac tatgccagcc
      301 atgcagaggt cgtgttcgaa aactgccgta taccgaaaga aaacctgctc ggccgcgaag
      361 gccagggctt caagctcgcg caggagcgcc tggggcccgg gcgcattcac cactgcatgc
      421 gctggcttgg catcagcgcg cgcgcgttcg agatgttgtg ccgccgcgcg aaccagcgca
      481 aaattcacaa tgacccttcg acaggctcag ggcgggtggc gggcatctat ttggccgaca
      541 gcgaaatcac gcagctctac gtcgccgagc tggcggccga gctgcacggg gcacgcctca
      601 tgactctgtc ggcagcatgg gcgattgaac acctcggcat gtcgaaggca cgcgacgaag
      661 tggcgatgat caagtttgtc gtggcgaacg tcatgaacaa gatactcgat cggtcactgc
      721 agatccatgg cgggctgggg atgtcagacg acacgattct ggcttactgg tttcggcacg
      781 aacgcgcggc gcgtatctac gacggcgccg acgaagtgca caaggcaagc ctgggcaaaa
      841 gaattctgcg cgactaccgg cgctaagaaa ccgcgtgggt agcctgcgcg aaccgggcac
      901 gttggggcgc atagcctgaa tgcaggggaa gctccagccg ggcaacagta tggctcttct
      961 ggtctgtcgc aatcgaaagg tatcttggat tcactgatac tgaacgcagt gccgcgatac
     1021 agagcatcag cccaaggccc gcaccttctg tttcgtcgat attttccatg atgaaatgct
     1081 ccgcgtgggt gcagcccata gctaggcgta gtttttcgcg cacgcggcgc tcttctgccg
     1141 atgacatcgg tacattgttg cgtatttcaa tgatgaaccg gtcatcgttg aagtcgatgg
     1201 tcgtggtgac gaccagcctg gcttcgcgtg ccttgtgact gtacgaggcg aacatgtctt
     1261 cagagaagat ctgcctgaac atgagtactc ccttctggta ctgttcagga tcttcgagca
     1321 gcaacccatt ttcggcgaag aacaatttct tgaagtttgc cttcgcggca ttcgtggtaa
     1381 gctctttcag gataatttcg agcgcgggtg aaaggtcggg cctgttaaac cggcttgtca
     1441 gtttgcgcgc atagttgcgc agcaggtctt cggttgcgtg gctcgcgtcg tgcgtagtga
     1501 cctccagtcg ggagtcttgc aggcagacac tacctgcggt ttggtcatgt tgatcggcga
     1561 gcatggcagg caatttctcg ttcagtaaca tccggtccag attgcagttc cgggacttcg
     1621 tggcacagat gtttcgtaaa cagctattta tcgtcagtct acaggtgcga actgaaccat
     1681 ggcgcgataa tgtgacataa cgtggctggt ggaaaaggcc agcagaccag gaaattggtg
     1741 gtacgagatc ttatgcgctg cgccggcgct gtgcataccg tgcgcggctg ggggtgtagc
     1801 cttcgtgcag cggaacttca acgcgcgcga gggtatgcgt gcgaaaatcc gtcgagatag
     1861 agaacagccg cgggtcaatc gaagacgagc gcatggtcgt caggcacagc ataatgccta
     1921 agcccgcccc ttcggtttcg tcggcgttgt ccatgatgaa ctggcccatg tcttcatagg
     1981 tcattgccgg ccgcagcttt tcgcgcgcgc gcttttcttc ttcgggcgtc atcggcacgt
     2041 tgttgcgtat ttcgagaatt acgcggtcgg ggtcatagtc gacagatatc agtacgagca
     2101 gctcagaggc ctgcgccttc tggcggtatt caatgaagat ttcttctgaa aatgtctcgc
     2161 gaaagcgtac cacaccctgc tcgtagtgct cggggtcgtc aatgcgcagc tggttctcgg
     2221 caaagaagat cttcttgaaa ttcgctttcg cggcgttggt ggtcagctct ttgagtatga
     2281 tttcgagtgt ggccgaaagc tccggccggc cggtttcctg cagtacacgg cgcaggtagc
     2341 tccgcagaaa atcttctgtc gagcgggtca ggccatatgc gcgtaccacc aggcggtttt
     2401 cgttttcaat aatttcgtcg gccgcttctt cgtcggcgcc cggggcccaa attctcgtca
     2461 ttaccatgtt cccatgtctc ttttttctgt ttccgaactt cctttttcgg gcgggtccgt
     2521 agcaaatact acatccctac ataactatat cgtgtacgaa agttcaccga ggccaaaaaa
     2581 gtagggggag ttgcctagtg acggggcggc ttcctcagag ggggtatttt gttttgacgc
     2641 gacgcggtgc acaaaaacag ggtcaaggca gttatgcaga tcaaagacaa tacctttctc
     2701 gtcaccggcg gcagttctgg cctgggcctt gcgaccgtgc agcggctggc tgaacacggg
     2761 gccaatgtgg ttgtagccga tttagctccc cctgctgacc tcaaacacag cggtgctgaa
     2821 ttcatcaaga ccagcgtgac caaccctgac gaaattcagg ctgcggttga cctcgccttt
     2881 agccgtttcg gttcgctgcg cggagtgatc aactgtgcgg gcatcggcaa cgtggtgaaa
     2941 actgtctcca agaagaaatt cggggccctc gacgcggcgc ggcagattgt cgagatcaac
     3001 ctgctcggca cttacaacgt gatcagtatt gcctcgacac ccatgagcaa gcaagatccg
     3061 ctcgcagacg gcgaacgcgg cgtcttcatt aatacagcgt cggtcgccgc gtttgacggc
     3121 aaagtgggcc aggcgcctta ctccgcctcg aagggtgggg tcgcgagcat gacgctgccg
     3181 cttgcacgcg aaatggcgca gtatggtttc cgtattatgg cgattgcacc gggcgcgttc
     3241 gaaacaccga tgactgcacg ccttcctggt aaggcgactg aagaactcgt cgatacgatt
     3301 ccgtttccca atcgtatggg acagcccgcc gagttcgcac aattggtgca atcgatcatt
     3361 gagaacacgt acctcaacgg cgaggtcatt cgcctcgacg gcggaataag aatgacatga
     3421 aggctctgcc gaagctcaag gtgcactggg gtatcgcggt cgcgatgctc ctggtttcgg
     3481 ggttgccggc gttctggttc acaaaattct ttttgcgcga tgcgtaccag ctgtaccgcg
     3541 cacaatcctg gcccatacgt gaggtcgtta tacagactgc ggcagaagac acactgcctg
     3601 aacccggtca gggtcatgga cctggcgcgc tctttgaacg tatcgagtac cgcttcgaat
     3661 cggacggaaa gcaatactta tcgcggcgat atggcccgtt ccagcgctgg aacttcggta
     3721 agaatgcgcc acgattcact gaaggtgccg cggttcgcgc gcacgtgaat ccggcagatc
     3781 tctacgagtc cgttctcgac aatcgtgtga gcgaaggttt ctggactgtg ctgtggttga
     3841 ccgtgctcac tgttgcgtgg ctagtctggg cattgctcac tgtacttcgc agtagacgat
     3901 ttttccgcgc gtttttggga cacctgctgc ttctgctgtc agcaggcatt ctcgcggccg
     3961 cattggttgc ctcggaagtt gagcgcaccc gcgactggtg gttcgcggtc ggtggttttt
     4021 tgtcaacgat agcacttgtc gggctctcgg tgattctttc gctctggaat cgtcgcgcat
     4081 atttgctcgc gggtatcggc atcgtggcgg gtttcgtgtc gatttttgtt ggcgtggcga
     4141 tacgcgaata ggaacttaat cctgcgatga agaatttgcc gccgtacacg atgcgctacc
     4201 cggttgcggg cctgctcgag atacgcgtgc ggccgcgtat cttttttccc ctgctcgtac
     4261 tcggcattat gctgccgctt tttctctttc gattgttctt ttcggtggag tttaaggcgt
     4321 tctggcgcat ggcgctgcgg gtattcggca ccgatttcgc aaatgaagaa gccgaagaca
     4381 tctacaatac tttcccggtg ttctccgcgc tgtcttttct gctgctcggt tcatggctcg
     4441 tgctgcacaa gcgtatcggt ttgatacagg tgacacgtga aggtgtgcgt atctttcgca
     4501 atccgcactt tttaccccgc tcgcaggtct ggataccgcg tgccgaaata aagagcctgc
     4561 gcgatgaaaa gctggcaagc cgcaacgacg acatcggtta cgtgctgcag cttgaaacac
     4621 acagcctggg taattttgaa ctggtacgca ttttcggttc tggaaacgac gaccggcagc
     4681 gttttggcca gcttggtaat gaaatacgcc gggtgctggg cggcatcggt taattttcca
     4741 ttgacgcccg atttaccgac aaggtaagtc ttttgcctat gcaagcagaa caagttgccg
     4801 cgctcaacaa gatcatgtcg atcattgacc agaaggcctc agaatacaaa caggaatata
     4861 tgcgcctgcc gcagtcaaag cggttcgcta accgcaagct gatactcgac ctaatcgatg
     4921 atgcgacaga actgggcaac gcaatgaaac ccgcgccgcg cgatgcgctc gaagacctca
     4981 agcgcctggc agagcagctc aacaggttgg cctaatatgc agcgttttgc tttatttctt
     5041 cttgcagtgc tgcttgcggg cgctgcaaca atgctaggcg tcgtctcggc atttgagcgc
     5101 acggctgcaa accccgaata ctactcgaat gccataaacc agattggcct gactgggttt
     5161 gctgtgccac tcgccgaaaa gctcaccggc cagaatggca aggtagctca ggtgctgcag
     5221 ctcggcatga accgacttga gccgcagctg aagcagcaga cagccgcaca cttgcagcaa
     5281 ttctttcagt atctgcgcgg caagaccgag aagtttgatc ttgtctacga catgaccacg
     5341 cttcaaagtg atactgcatt caccggcgaa gtcgttgaaa ctttgcagaa ggacagcaag
     5401 ttaggccttt tgcccagaag cctgttgcag cccgttgcgc agaagctcac gaaccagctg
     5461 ccaggtaaga ttgacctgct cagtctcgca ggtcgtgatg aaggctccat ggcctcgctg
     5521 cgtaaagatg cgcaggcgtg gttgtcgact gctgacaggt ggttaattat cagcgcggcg
     5581 ctttgcctgc tttttgtcgt cctgatcttt ttggctgcgc gttcgattcc ccgcacactg
     5641 actacggttg ccgttggtct gctggttgcg gctgccgtgc tcatgctgcc ttgggttttt
     5701 tccgacgcga ttctcgccat gctgcggttg aaaggcgcga tctatgaatt tctgcagcag
     5761 accggtctgt ggcaggcact cgcagctgag ctgagcaaag agtaccttgt ttctccggtc
     5821 accgcagcgg caaccggagt tgtctttctc gttggtggca tcagcctgcg ccggtttgtt
     5881 ccctattctg gcaaaaaata acaggggcaa ccttggcgcg cgtcctgaaa ctgccgctgc
     5941 ttcttacgct gctgtctctt tggccggcac tgcaggcaac acaaccacgc gcgggtgttg
     6001 ccttttcgcc cggttacctg acggcgcagg gtaccgttgc ctatgctgcc agcgccgagt
     6061 gggagttctc cctcggggct cgcatcgcgc tgcgcagcga cgtccacgcg ctgcttgcgc
     6121 gtccacagga cagtaatctc acgcagaact accagggttt tgtcggcctc ctttactatt
     6181 tcgatcaatg gtttggcagc cagcctttcg ccggcttcca gccagggttt ggtgtcggca
     6241 aagtgaatac tcctgcaaac ccggggttca ggctttaccc ggctatgtcg ccggtcttcg
     6301 gtctgcatct gccgttgttg gggacgctgc agctcacatt cgcgctgcgc tacgtctttg
     6361 gtgagctgca ctaccgcgat actggtgctg tctacctgag tgaactcaga atcgcgcttg
     6421 gcttgggcgc taacctgtga gagattgtta gtcaaatggc ctgaagacta tgacgaaagg
     6481 gcgttgcgtc atctcgtcaa tgcaccgttt tttctcagta tcatcggcct tgtcggctgg
     6541 atctacgctg gcttttcgac tgacgatctt tttgttcggc accgcgctcg cgcccatttc
     6601 gattcttggc ctcgcggcga tcaggctgag cgaaaaagtc ggttcgggaa gcgacgcgcc
     6661 ggtgcgcatc gtgctgctca ttgcgctcat gctcgtattg ggaatttacc tgagcctgtt
     6721 actggcccgc acattgcgta ctcccttgcg cgagctgcag cgcgccgcag cccagatacg
     6781 cggcggcgat taccacgtgc ggctgccggt aacgaccaac gatgaagtgg gccgtctcac
     6841 gcagggtatc aacgaaatgg ctgccggcct gcgggaacgc gaacatattc gcgagacatt
     6901 tggtcgcgct gtcgcctgtg cgctcgacat gcttgcgaca ctcgagcgcc tcaatgcaga
     6961 gctggctgca gaaaaaattc ccgaagtcag aattggcatc ggtctgcact gtggtccggt
     7021 cacggcgggt agtgttgggt ctaatgatcg tctcgagtat acggttatcg gcgacacggt
     7081 gaatactgcc tcacgcattg agggcctgtg caagaaggct gggcggcctc tcttgttgag
     7141 cagcgcggtt gcacaggctt gcggcgatga gtgggagttc gtgcgtcttg gcaaggcgcg
     7201 cgtcaagggc aagactgacg cgctggaaat tctgacagtt aagtcataag ctggctagac
     7261 agacccgcca gtttatgact cctcgtgtag cgattgctac agaaataatt gacggattgt
     7321 tcatttttct gggtgagaga cgcacagcgg ccggcgttgc ccgccgcagg aggatacacc
     7381 atggcaatga ctgtcagaaa aaataaagaa acattcgcag agcccgtacc agcgcactgg
     7441 gcaggcaaag acgcgctgca gagccacgtg ctcaatgtgc tgagcctcat gtttcccgat
     7501 ggcgaagcat tctttatacg ttctgtgaaa cacttcgccg accgcgtgac cgaccagaaa
     7561 atgcaggcag atatcaaggc cttcgtcggg caagagatgc agcacagcat ggctcacgaa
     7621 aggtttaaca aagcagtcgc cgaaaatgtt ggcaacatgg attggttcat gtggcttttt
     7681 accgtgccga ctttcgaatg gctagaaccc gttgttcgca agaatctcgg tttcgagata
     7741 ttgcacaagt gggcgctcgc catcactgcg gcagcggaaa acatgaccgc cggttttgcg
     7801 cagtcgctct ttcagccggg tgaactcgaa aagattgccc gccccgacgt gcgcgaactc
     7861 tatgcatggc acgctgccga agagatggaa caccgtgacc tggcgtatga cctctaccag
     7921 caggtgggtg gcgactacgc gacgcgcatg gccggtatgg cattcgccta cggcatcatt
     7981 gcggcttatg ttgcagtggg taccacgtac ctcgtgctca acgacaaaca gtttccatgg
     8041 gcgactctgc cacagcagct ggttgacctg gtcacgaaag agaattcact cggccgcgtt
     8101 tttctcgagg gtatggtcga ttacatacga cctgactttc accccagcca gcacccgatg
     8161 aatgccgagg cggtggcgta cctggaaaag atggcctcgt aagcgctgtg cttacgcttg
     8221 tattttatta aacggcctct cggcctcgag caggtaggca atctcgagca gcgtgcgttc
     8281 gttgccgtgc gctgcctgaa actggatacc gatgggaaga ccttcagcgc tgtgggcaag
     8341 cggcaatgaa attgccgggc tgcccgtcgc attgttcagc ggcgtgaaca tgatgtagcg
     8401 cgacagtttt tccatcaggt catcataacc ctggccaggg ttgaggtgac caagttttgg
     8461 taccgtatgc cccagtacag gcgagagtac cgcgtcgtaa ggtttcatta tttctgcgta
     8521 caggttagcc gagcggtgca ggcgccagag cgccgctggt gtgtgcaaca ggttcttgcg
     8581 gtagtgctgc acgaggccct tgctaaacgg gtcgagcctg tcataatcaa aatcgctccc
     8641 tagcagaaat ttgccgagtg agcccatcat gaatgccagc attgcccagt agagggtgaa
     8701 atcgtcgata aacgtcgccg gcagattcag cgtgattggt tctacgcggt ggccgagctt
     8761 ttccatcagg cgggcaatgt cttcgacggc tgcgcgcgtt tcggggcagg tcggcgtccc
     8821 cgtgatcgag tcgatcacca ggccaatgcg gagttttttc tgtgacggtc cctcaacctt
     8881 accgacaggt ttgagtctcg ggttcttgta gacggactcc atgccataca tgaactgcgc
     8941 cgtatcgcgc acgctgcgcg tgacgacacc ctcgctcacg atattgatcg gcatcgactg
     9001 cgccgacttg ttgttgaggt gccggttgcg cgtgggcttc aggccgacaa ggccgcagca
     9061 cgcagcaggt acgcgaatcg aaccaccacc atcgttcgcg tgtgcaatgg gcacgacgcc
     9121 ggccgccacc agcgccgctg aaccgcccga cgacccaccg ggcgaatagt cggtattcca
     9181 cgggttgcgc gtggggtggt cgccttcaaa ctcggtcgag gcgttaaaac caaattcggg
     9241 catctggctt ttgccgagca cggtaaagcc ctgcgaaagg tattgttctg tataaggacc
     9301 gttttcttcg gctgctgccg gcgtgaagga acgagtgccg ttacgcgtgg gaaatccctc
     9361 gaggtcggca ttgtctttga caaacgtggg aactcccgca aaaacaccgg gcttcgcagg
     9421 caggtgcgtc tgcgccagcg cctggtcgta tgccgcgatc tcgatcgcat tcagcttatc
     9481 ggcgacgctg cgtgcgcggg cgattgccgc cttggcgacc tcggtcgcat ccagttcgcc
     9541 cttgcggatg agttcagcga gcgcaacggc gtcatgtgtg gccatcgcgt catcggtgaa
     9601 ggcgtgcaca cgtttcgaag tatctgctgc cggggtcgct gtgctcttag ccatagcgac
     9661 aggcctgctc tgcgacaggc gcggtcaaga gcaaggcagg ttactcacca cggtatctct
     9721 ttcttatcgc gcaggaattt tcctgttggt ggtttttcgg ttgtggcaag ccagacgatc
     9781 gtttcagcac cctgttcgac ttcgcgggtc gcgcgttcgc cgcccatgtc ggtcttgacc
     9841 cagccagggc agacagagtt gacgctgatc ttatcttttg ccaggtcatt ggcgaagatg
     9901 cgcgtgacgg cgttgagcgc cgtcttcgaa atacggtaac ccgggtaacc gccatccatc
     9961 tcggtgagac cacccatgcc gctcgagagg ttgataatac gcgcatcgcc cgattttctg
    10021 agcaggggca acaacacttc aatcatgcgg taggggccga gcacattggt gtcgaaggtt
    10081 tgccgtatga tgtcgatgtc ggctttcgtg ctgtcgctgc tgaccagaaa gatgccggcg
    10141 ttgttcacga gcacgtcgag tttgccgtaa cgctcatcga catattgcac gagcttgcgc
    10201 acctgcgcct cgtcgcgcac atcgcaggcg aaggcatcgg taatgacacc ttcggccgcg
    10261 aggtcgtcct gcgcgtcgcg cacatggtcg gcgttacgcc cggtgagaat cgtcttgtga
    10321 cccacgcgcg ccaattggcg aatggtttct ttgccgatgc cccggttaga accggtgacg
    10381 agaataactt tcatggagtc agtttattcc actacactta gcgtggcgag tgaaaacggc
    10441 taatgtcccc ctcccgacct cccccgcaag cggggcgctc cggcgggcgg agcctgcggg
    10501 tgccgaaggc ggagggagac gaagggagaa agcatttcag ccctaacaca aacctgttct
    10561 ttggcaaata acatgtctac gcgcgccttg attctttctg gcggtggtgc ccgcggcgcc
    10621 taccaggctg gggtctaccg ttacctgtcc gaaatcaact ggaaacctga cctgatcagc
    10681 ggcacctctg tcggggctat caatgcgtgc gctattggct caggttttga tgcccagaag
    10741 ctgcagacgt tctggctgaa tattgcacaa gaccacgtct tcgcgttttc gttttggaga
    10801 aagatctggc attttatttc acggcggggt tattcagcgg tgctcgatac gacgcccctc
    10861 agaaaatttc tgtcgaataa tatcgatata cgcgcgctca ccgagagcga tatagaagtt
    10921 gtcatctgcg cgatcaacat cgtgcgctct aagctccgtt tttttacgaa caaagaaatc
    10981 ggcgtcgaag agattatggc ctcgagtgct ataccgctgc ttttcccgtg gcaagaaata
    11041 gatggtgaac cacattggga cggcggcatc atggcgaaca cgcctatctt accggcgttg
    11101 gagcgcggct cgcgtgagat cgtcgtggtg ctgctttcgc cggtaggcgg catgagtatg
    11161 gcgataccaa aaagcagaaa gaaaatagct gaactgattc tcg
//
LOCUS       DAOQJF010000070        10922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3082_length_10922_cov_5.780804, whole genome shotgun sequence.
ACCESSION   DAOQJF010000070 DAOQJF010000000
VERSION     DAOQJF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_10922_cov_5.780804"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLF85_05475"
     CDS             complement(<1..612)
                     /locus_tag="PLF85_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNE19144.1"
                     /translation="MIIELNGDAFRVEVVRSSTRRASVSLKYAEHGNFILSVPVATPE
                     RYIREFLEQKRGWMQKIRGKARERDRAQTITPGSHITTEFYSLIAAEDSQLAFPQYRV
                     ERRKAEQRSVFHLAPGFFAAENQPKLWQNLEKYLLAQLVKLGSPSLVERTQYWADHHK
                     IRVREVFVRVQKSRLGYCTHDDRIMLNARLLFAPQKIRDYVIHH"
     gene            complement(602..1435)
                     /locus_tag="PLF85_05480"
     CDS             complement(602..1435)
                     /locus_tag="PLF85_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE19145.1"
                     /translation="MIEFRHKDIHVDAIGRGGIETCYMLPQWQIAFDTGRCPDALVDI
                     PRVFLTHGHLDHAAGLPYYISQRSLKRLPMPEICCPTEIVQPLSEILHLWQKIEGFTY
                     EIAIKGMDPGDEVSLRKDLYVRALVSHHRVPCRGYAVMRKTRKLKEEFLSVPGREIKR
                     MKDRGDEIFEDKDIPVFAFSGDTTIEFLQDNPLAQEAQVLFIECTYIDDKRPVSRARE
                     WGHIHLDEIIANRHLFKNEKIVLTHFSKRYHRRYITQVLGKKIDDADFLSRVFVLAHD
                     Y"
     gene            complement(1444..2199)
                     /locus_tag="PLF85_05485"
     CDS             complement(1444..2199)
                     /locus_tag="PLF85_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19146.1"
                     /translation="MRRTKHLRLVLVSSLLLVAAACSPKPAQIAYLAPLDRLQEGFLD
                     ATTYQVLGYGRALDLTQPIDPKTAFFPVSVGVAFDHEEFQSYNTEVQNLLKARKPVNG
                     VPFAEVMAAEANQLNPQEVNLAAIDEKIRAPMEIKRALFDNACTNARILALYRWLIGD
                     ATQMKLLHGATLPPEGLQKTTLDPRFYPPRAHYVAESAAILKNLDQAMAKKKFRYEIV
                     AETFSKPEQLECKVAIHIHRQNLQVSMPFLAPL"
     gene            2411..4345
                     /locus_tag="PLF85_05490"
     CDS             2411..4345
                     /locus_tag="PLF85_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE19147.1"
                     /translation="MEYVKLNVQTYVEAVQQSFKKNAARLALRYREGSEDFKTMTYAE
                     LEKQVNLVAAGLAAIGVKPKDHIGLIADVSHYWMTAHLAIQLNGAADVPRGTDSTGPE
                     IGYILDHSGSTIVFVHNAAQVKKIDAGLKGFKGKVKKYIVLNDEKVKGTNVMTLSELK
                     AEGEKLVKKGGKALAEFESRAGKVVPSDLSCIVYTSGTTGEPKGVMLSHGNFASQMRV
                     IPEVLKIGPSDSGLTLLPPWHVFGRITEMMFISIGAAITYTDIKHIGEDMRALKPTYV
                     PAVPRIWEGVYNKIIGNVKKAGKEPVFNKFKTAALKYNHFVSMLTGKEKIPLGRNVVS
                     TIGARVLGLLGAIAWFLPKKLGDILVFKKVLAATGGNLKASLSGGGALPSYIDDFFRA
                     IGVRILEGYGLTETSPVLSVRRMERLIPGTVGPLIAGTEYKLIDLEGRDVTRIPGAKG
                     TLHVRGPQIMQGYYKNPKKTAEVLTPDGWFNTGDLVVMNYSGEISIVGRSKDTLVLVG
                     GENVEPTPIEEKLKESPFIDHVMLVSRAFGEDQKVLGGLIVPNAENLAEWCKKNGIGG
                     DLAQWVKEPKVHAMYRQEINKSINAENGFKNFEKVGVFTLLKKPFEQGDELNNTLKVR
                     RHVVSEKYAKLIDEMYASEK"
     gene            4391..6265
                     /locus_tag="PLF85_05495"
     CDS             4391..6265
                     /locus_tag="PLF85_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNE19148.1"
                     /translation="MLTSIIIDRVGKTNVFNIVQESGVNNPALKPSERLQSVIDDDLI
                     GEYLEELGRIANISRSLSSLPRGNEEHQALIFQHLNLSHKLREIGEALFKQFFPPSLQ
                     DYIRNSEQSYLYFHVDAALASLPLEILHDGTAFLWEKFYLGKAIKGQEIAASDFHPRE
                     IINMLIIADPREDLDWARREGEMLFEHLGAFVSPKKINMTLIGGKTVTKLNILNSILD
                     KDIIHYAGHLHYSGNPDENGWLLADGKILYAREFKMSGAQPKLIFCNSCLSARSGQVV
                     SDASWYAHFAAAFIRAGRTSYVGTNWELPDRQPTLEFTTQFYDNIFQGKSLGESLQQS
                     RSYAREHFSLNDLTWASYLLMGNPMQTVFRAESLLPDVTRNMLETEDVIAHYPYPIAG
                     AFAGFHRRFTELNNQGKEGDEELLKSLFHLYTECLFFLTGLVLANYRVFNFPKPIAFP
                     YPNVEKSIAALFSALGAIRAIKAQPLAVNLLETLYIHKDNLEKIATLRKKYQAGEVRD
                     TDFETYTITVQYLMESLLIDLDFLRHYGFYLIVEPGHRQLSFHGVEQYHTHRDILLPT
                     QANAVTYTELLEKTSYLVGRCVFYSPVKKTFLDLSPFMRINAEEDGSYTFAFTKAKAV
                     "
     gene            complement(6270..6854)
                     /locus_tag="PLF85_05500"
     CDS             complement(6270..6854)
                     /locus_tag="PLF85_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19149.1"
                     /translation="MRWLLAAHEKEIPGFLDFRVSDWQPRAVGVGQFAALSRLSALLA
                     VEKPAAILLAGTAGATEPAAVGQHFAVQHFAYPSIAGEDLPEFLERAFTTEPAISVGG
                     FSPATVLQNHGVSTDLTKFVSNAGYIPPDYPRPILENMEAAALAQLCRRLQIPFTALI
                     AVTNTIGPIARLQWKQNFRRAGEQLAVSLRQVLG"
     gene            complement(6858..7532)
                     /locus_tag="PLF85_05505"
     CDS             complement(6858..7532)
                     /locus_tag="PLF85_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNE19150.1"
                     /translation="MHRKERLYITLAGFFITNAILAEIVGGKIFTFDIPPLLWFTQTT
                     ANLSVGVLVWPIVFIMTDIINEYYGIAGVRRVTLLTIVLISYVFVALFFMGETRAASF
                     SAINDENFNVVFGTSQSIIIGSLVAFAVGQFLDFFIFHRLKMMTGNSHLWLRATGSTV
                     FSQLIDTFIVLYIAFVIPGKWTMADFLKVASSNYLYKLGIAVAVTPVIYLVHAIIDNY
                     LGKEEH"
     gene            7626..8690
                     /locus_tag="PLF85_05510"
     CDS             7626..8690
                     /locus_tag="PLF85_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19151.1"
                     /translation="MEHSVPDKKEQRKILIGKLVHLGIIMAVATVLGVFAIKIILSHY
                     KLDHKPMELGGDARGFATKSRSGTYMKLSNLNFRLTDEINMQVKELVGEAVPKTKNGI
                     INFDDINSFRIDILSGEAFVRTEIMEYIFNNLVFNYDGSPLKNMKMEFFDDEDNGKPM
                     KKLRLTGDMKLVVWLGFEMVGKMYLDREKTLMVIEAERIKSLGNPYTKTLLDTVGLNM
                     EKLLPVPSGRGITMMGNKIIVEPFKIFPPPQIGGFISDMRVQSNGLQLFFSSKTKVNF
                     PPLPVKDVKNYLFLYQGDVKFGKLMMVDARLQMVDMSQKNDFDFYLKKYLVPLGAGHS
                     KILRDGSVVAYIPDYRDVAR"
     gene            8693..9133
                     /locus_tag="PLF85_05515"
     CDS             8693..9133
                     /locus_tag="PLF85_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19152.1"
                     /translation="MRCAVPGVILPLLLAFACSRKAEVLPEGAARVTRSDSLVENGNQ
                     SYELRSYTFQLKLAEGEASFVCEAKKFTGAAEALAAAEKSIAQRPDEQQRNSSAWYAR
                     AGLSQWLLTGRTLTWCMQSGSGSASREAAQAKLREAFLKRFPEA"
     gene            9130..10086
                     /locus_tag="PLF85_05520"
     CDS             9130..10086
                     /locus_tag="PLF85_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19153.1"
                     /translation="MMMTIFQRIFSSRIVAGAILLVLSCTRPQAESQRVAEPGQQFQA
                     YWNAGKAELARYELRQARYGQVHPGEMVVITVTEPFRTDRQVKAESAEGAQHAVNVLK
                     VQQMRRFATGIYDYALTTTSFRPFAAQPAAVLKVTGSAVDWCGHAWLQLNRRKDSFAV
                     ESRSYFENPGDMEYSVSAALSEDEIWQRIRTNPAALPVGKITVVPSLMSARLRHRATQ
                     VETARAELQQFARLKQTEYSLVYGQGQDDERQVAFIFESEFPHRVLEYRELYSDVIGG
                     GGKKDKLSTVARLKKTILSPYWQQHDLIHKKDRKDFGVRGFD"
     gene            10126..>10922
                     /locus_tag="PLF85_05525"
     CDS             10126..>10922
                     /locus_tag="PLF85_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19154.1"
                     /translation="MRVFIVGAGAVSGGAAIAAAKSLGAEIIATTSRAENVAGADHTI
                     YGIDLERSDAVDKMLADKKLTAKPVDFVVYIPARGAVGMDVTESTADMVMPSLEYSVI
                     PYLKLAKALKPKRTIALSGFMALPTLTKIYGAMTFTKVAMEQLAVNNPDKLQIIRIGM
                     FLSNSVRGIAILAQRRFMREPDFQPEWRAQWKASGKKFSQFFYDMNYEQEEQIYRQHS
                     NGVPFRPTLPEDITNGFLMAFKGEQAPIINVLGPWLWTEQQMPELPE"
BASE COUNT         2540 a         2955 c         3088 g         2339 t
ORIGIN      
        1 gtggtgaatc acataatcgc ggattttctg cggcgcgaac agcagtctcg cattgagcat
       61 gatacggtcg tcatgcgtgc agtagccgag gcgtgatttc tgcaccctga caaacacctc
      121 gcgcacgcga atcttgtggt ggtctgccca gtactgtgtg cgctcgacca ggctcgggct
      181 gccaagtttc accagctgcg cgagcaggta cttctcgagg ttctgccaga gtttgggctg
      241 gttctccgcg gcgaagaaac ccggcgcaag gtgaaagacc gaacgctgtt cggccttgcg
      301 ccgttcgacc cggtactgcg gaaacgcgag ctggctgtct tctgcggcga tgaggctgta
      361 gaactcggtg gtaatatggc tgccgggtgt aatggtttgc gcacgatcac gctccctcgc
      421 cttacccctg atcttttgca tccagccgcg tttctgttcg aggaactcgc ggatgtaacg
      481 ctcgggggtc gctaccggta ccgagaggat aaagttgcca tgctcggcgt acttcagtga
      541 aacgcttgca cgccgtgtcg acgaacggac gacttcgaca cggaacgcat caccgtttag
      601 ttcaataatc atgggcgagc acaaagaccc tgcttaagaa atcggcgtcg tcgatcttct
      661 tgccgagcac ctgcgtgatg tagcggcggt ggtagcgctt cgagaaatga gtgagtacga
      721 ttttttcgtt tttgaacagg tgccggttgg cgattatttc gtcgaggtgg atgtgtcccc
      781 actcgcgcgc ccgcgacacg gggcgtttgt cgtcgatgta ggtgcactcg ataaaaagca
      841 cctgcgcctc ttgcgcgagc gggttatcct gcaaaaactc aatcgtcgta tcgcccgaga
      901 aggcaaacac gggaatatct ttgtcttcaa aaatttcatc gccgcggtct ttcatgcgct
      961 tgatttcgcg ccccggcacc gacagaaatt cttctttgag tttgcgggtc ttgcgcatca
     1021 cggcataacc ccggcagggt acccggtggt gcgaaaccag tgcccgcaca tacaggtcct
     1081 tgcgtagtga aacttcatcg cctgggtcca tgcccttgat ggcaatttcg taggtgaacc
     1141 cctcgatctt ctgccagagg tgtaatattt cgctgagcgg ttgtacgatt tcagtcgggc
     1201 aacagatctc gggcatcggc aaacgcttca ggctgcgctg gctgatgtag tagggtagtc
     1261 cggccgcgtg gtcgaggtgg ccgtgcgtca gaaacacccg cggaatatcg accagcgcat
     1321 cggggcagcg gccggtgtcg aaggcaatct gccactgcgg cagcatgtag catgtctcga
     1381 tgccgccgcg gccaatggca tcgacgtgaa tatctttatg cctgaactcg atcacccggt
     1441 tctctagagg ggggccagaa agggcatgct gacctgcagg ttctgccggt gaatatggat
     1501 ggcgaccttg cattccagct gctcgggctt tgagaaggtt tcagcaacaa tctcatagcg
     1561 gaattttttc tttgccattg cctggtcaag gttcttcagg atcgccgccg attcggccac
     1621 gtagtgtgcc cgcggtggat agaagcgcgg atcaagcgtc gtcttctgta agccttcagg
     1681 cggcagtgtc gcgccgtgca gcagtttcat ctgtgtcgcg tcgccgatca gccagcggta
     1741 gagagccagg atgcgcgcgt tggtgcaggc attgtcaaac agcgcgcgtt tgatctccat
     1801 cggcgcgcgg attttttcgt cgattgctgc caggttgact tcctgtgggt tgagctggtt
     1861 ggcctcggcg gccatgactt ctgcaaaagg cactccgttc accggcttac gcgctttcag
     1921 caggttctgc acttcggtat tgtacgattg aaactcttcg tggtcaaaag ctacaccgac
     1981 agacacggga aaaaatgctg tctttggatc gatgggttgt gtcaggtcga gcgcacgacc
     2041 ataacccagt acctggtagg tcgtggcgtc gagaaaacct tcctgcagcc tgtcgagcgg
     2101 agcgaggtag gcaatttgcg ccggcttggg tgaacaggcc gcagccacga gtagcaggct
     2161 gctcaccagc acgagccgta gatgtttcgt ccgccgcatg agacataacc ccgcgcatct
     2221 cgctgtcacg gcggcaagtc gaaattcgcg acccaggccg cgttgcgacc cattattttc
     2281 agcgtagaat ataaatcggg caactgtgtt tttgcttgac ccaaaaagtt tcaacccggc
     2341 tctgcaccat ctaatcccgg gtcgctggcg tctgcgttag tcaccccgga gcaaacacag
     2401 agaggaaccc atggaatatg taaagctcaa cgtgcagacc tatgtcgaag cagtgcagca
     2461 gtcgttcaag aagaacgcgg cacgcctcgc gctgcgctac cgcgaaggaa gcgaagactt
     2521 caagacaatg acttatgccg aactcgagaa acaggtgaac ctggttgcag cgggtcttgc
     2581 ggcaattgga gttaaaccaa aagatcatat cggtctgata gcagacgtga gccactactg
     2641 gatgactgcg cacctcgcga tacagctgaa cggtgcagca gatgttcccc gcggtacaga
     2701 ttctactggt cctgaaatcg gttacatcct cgaccattcg ggcagtacga ttgtcttcgt
     2761 gcacaacgct gcgcaggtga aaaaaatcga cgccggtctc aaggggttta agggcaaggt
     2821 gaagaaatac atcgtgctca acgacgaaaa agtcaaaggc accaacgtca tgactctgag
     2881 cgagcttaag gcagaaggcg aaaagctcgt caagaaaggc ggcaaggctc tcgccgagtt
     2941 tgaatcgcgc gccggcaagg tagttccttc agatctgtcg tgcattgtct acacgtctgg
     3001 taccacgggc gaaccaaagg gagtgatgct gagccacggc aactttgctt ctcagatgcg
     3061 ggtaattcct gaagttctga agattggtcc ttctgacagc gggctcaccc tgctgcctcc
     3121 ctggcacgtg tttggtcgca ttaccgagat gatgttcatc tctatcggcg ccgccattac
     3181 ctacaccgat atcaagcaca tcggcgaaga tatgcgcgcg ctgaagccaa cgtatgttcc
     3241 agccgtaccg cgtatctggg aaggcgtcta caacaagatc attggcaacg tcaagaaggc
     3301 gggaaaagaa ccggtcttca acaagttcaa gacggctgcg ctgaaataca accacttcgt
     3361 gtcgatgctc acaggtaaag agaagatccc actgggacgt aatgtagtgt caactatcgg
     3421 tgcgcgtgta ctgggtcttc tgggggctat cgcatggttt ctgccaaaga aactcggtga
     3481 tatcctggta ttcaagaaag tactcgctgc aaccggcggc aacctcaagg cttctctgtc
     3541 aggtggtgga gctctgccat cttacatcga cgatttcttc cgcgcgatcg gcgtgcgcat
     3601 tcttgaaggt tacggcctga cagaaacttc tccggtactg tcggtgcgcc gcatggagcg
     3661 tctgattccc ggcactgtcg gcccgttgat tgccggtact gagtacaaac tcatcgacct
     3721 cgaaggccgc gacgtgacac gcattccagg cgccaaaggt acactgcacg tgcgtggacc
     3781 gcagatcatg cagggttatt acaagaaccc taagaaaacc gctgaagtac tgacgccaga
     3841 cggctggttc aataccggtg accttgtggt catgaactac tcgggtgaaa tcagcattgt
     3901 gggtcgctca aaggacactc tcgtactcgt cggtggcgaa aacgtcgaac caacccccat
     3961 cgaagaaaaa ctgaaagaaa gtccattcat cgaccacgtg atgctcgtgt cgcgtgcctt
     4021 tggcgaagac cagaaggtgc tcggtgggct tatcgtgccc aatgcagaaa atcttgctga
     4081 gtggtgcaag aagaacggca tcggtggcga cctggcgcag tgggtgaaag agccgaaagt
     4141 gcacgcgatg taccgccagg agatcaacaa gtccatcaac gctgagaacg gcttcaagaa
     4201 ttttgagaaa gtgggagttt ttactctgct gaagaagcct ttcgagcagg gcgatgagct
     4261 caacaatacg ctcaaagtgc gccgccacgt cgtttcagag aaatacgcaa agctgatcga
     4321 cgagatgtac gcttcagaaa aataagcatc ctttccggtg cggttcaagc cgcaccggaa
     4381 agtctatgac atgctgacct caatcatcat cgaccgtgtc ggcaaaacca acgttttcaa
     4441 tattgtacag gaaagcgggg tcaataaccc tgcgctcaag ccgagtgaaa ggctgcagtc
     4501 agtcattgat gatgacctga tcggtgaata cctcgaagag ctgggccgca tcgcgaacat
     4561 ctctcgctcc ctctcgtcgc tgccgcgcgg caacgaagag caccaggcgc tgatcttcca
     4621 gcacctgaac ctttcgcaca agttgcgcga aatcggtgaa gcactcttta agcagttttt
     4681 tccgccgtcg ttacaggatt acatacgcaa ctcggagcag agctacctgt attttcacgt
     4741 cgatgccgcg ctggcttcgt tgccgctcga aattctgcac gacggcaccg ccttcctgtg
     4801 ggagaaattc tatctcggta aggcgatcaa gggacaggaa atcgcggcca gcgattttca
     4861 cccgcgcgaa atcatcaata tgctgattat tgccgacccg cgcgaagatc tcgactgggc
     4921 acgccgcgag ggcgagatgc tgttcgagca cctcggggca tttgtcagcc cgaagaagat
     4981 caacatgaca ctgattggcg gcaagaccgt cacgaagctg aacatactga attctatcct
     5041 cgacaaagat atcatccact acgcgggtca cctgcactac agcggtaacc cagacgagaa
     5101 cggctggttg ctcgccgacg gcaaaattct ttatgcgcgt gaattcaaga tgagcggcgc
     5161 acagcccaag ctgatcttct gcaattcatg cctgagcgcc cgttcgggac aggtggtcag
     5221 cgacgcgtcg tggtacgcgc actttgccgc ggcgttcata cgtgcgggtc gcacgagcta
     5281 cgtgggcacc aactgggagc ttcccgaccg gcaaccgact ctcgaattta ccacgcagtt
     5341 ctacgataat atcttccagg gtaagtcgct cggcgagtcg ctgcagcaga gccggtcgta
     5401 tgcgcgcgag catttttcgc tcaatgacct gacctgggcc tcgtacctgc tcatgggcaa
     5461 cccgatgcag acggtctttc gcgctgaatc gctgctgcct gacgtgacgc gcaacatgct
     5521 cgaaaccgaa gatgtcattg cgcattaccc ttacccgatt gcgggtgcgt ttgccggctt
     5581 tcaccgtcgc tttaccgagc tgaataacca gggcaaagaa ggcgacgaag agctgctcaa
     5641 gtcgctcttt catctctata ccgagtgcct cttctttctg acgggcctgg tgctcgccaa
     5701 ctaccgcgtg ttcaattttc ccaagccgat tgcgtttcct tatcccaacg tcgaaaaaag
     5761 tatcgctgcg cttttttctg cgctcggcgc gatacgcgcc atcaaggcac agccgcttgc
     5821 ggtgaacctg ctcgaaacat tgtatataca taaggacaac ctcgaaaaaa tcgctaccct
     5881 gcgtaaaaaa tatcaggccg gcgaagtgcg cgatacagat tttgagactt atacgattac
     5941 ggtgcagtac ctgatggagt cgctgttgat cgacctcgac tttttacggc actacggctt
     6001 ctacctcatt gtcgaaccgg ggcaccgcca gttgagtttt cacggtgtgg agcagtacca
     6061 cacgcaccgc gatattctgc tgccaacgca ggcaaacgca gtcacctata ccgaattgct
     6121 cgaaaaaact tcgtaccttg tgggccggtg cgtattctat tcaccggtga aaaaaacctt
     6181 tctcgatctg tcgccgttta tgcgtatcaa tgccgaagaa gacggttcgt acacctttgc
     6241 cttcaccaag gcaaaggcag tgtaactatt taacccagta cttgcctgag tgaaacagcc
     6301 agctgttcac ccgcgcggcg gaagttctgt ttccattgta aacgcgcaat ggggccaatg
     6361 gtgtttgtca cggcaatgag cgctgtgaaa ggtatctgca acctgcggca cagctgcgcg
     6421 agagctgctg cttccatgtt ttccagtatc ggtctcgggt aatctggggg gatgtacccc
     6481 gcatttgaaa caaattttgt caggtcggtg ctgacaccat ggttctgcaa taccgtagca
     6541 ggggaaaatc cccctacaga tattgctggt tcggtcgtga aggcccgctc taagaattcg
     6601 ggcaggtctt caccggcgat actcgggtaa gcaaagtgct gcacggcaaa gtgctgcccg
     6661 acggccgctg gttctgttgc gcctgcagtg ccggccagta gtatcgccgc tggtttttcg
     6721 accgccaaaa gcgcagaaag ccgcgagagc gcagcgaact ggccaacacc cacggcacgc
     6781 ggctgccagt cgctgactcg aaaatccaaa aagccgggaa tttctttttc atgcgcagcc
     6841 agcagccagc gcatttctca atgctcttct ttgccgagat agttgtcgat gatcgcgtgc
     6901 accaggtaga tcacgggggt cacagcgact gcgatcccca gcttatagag atagttggag
     6961 ctcgcgacct tgaggaaatc agccattgtc cacttgcccg gtataacgaa cgctatgtag
     7021 agcacgatga aagtgtcgat cagctggctg aaaaccgtgc tgcccgtagc gcgcagccag
     7081 aggtgtgaat tgccggtcat catcttgagg cgatggaaaa tgaagaagtc gagaaactgg
     7141 ccaacagcga aggcaaccag tgaaccgatg atgatgctct gtgacgtacc aaaaaccaca
     7201 ttgaagtttt cgtcgttgat cgccgaaaac gacgctgccc gggtttcgcc catgaagaac
     7261 agcgccacga aaacgtaaga gatcaggaca atcgtgagca gcgtcacccg gcgcacgccg
     7321 gcgataccgt aatactcgtt gataatgtcg gtcatgatga agactatcgg ccagacgagc
     7381 accccgacag agaggttggc agtggtctgg gtgaaccaga gcagtggcgg aatatcaaag
     7441 gtgaaaattt ttccacccac aatctctgcg agaatggcat tagttataaa aaagcctgcg
     7501 agcgtgatat agaggcgttc tttgcgatgc ataaagattt gttctcaggg cgcggcgcga
     7561 aattcccgac ggccttaact cactttatga atttgacaca cgcgtcaaag aaaaaacctt
     7621 gcgccatgga acactctgtc cccgacaaaa aggagcagcg caaaatactg attggcaagc
     7681 tcgtccacct gggtattatc atggcggttg cgacggtgct gggcgtgttc gccatcaaga
     7741 ttatcctgtc gcactacaag ctagaccata aaccgatgga gctgggggga gatgcacgtg
     7801 gttttgccac caagtcgcgg tctggtacct acatgaagct ctctaacctg aatttcaggc
     7861 tgaccgacga aattaacatg caggtgaagg agctcgtcgg cgaagcggta ccaaagacca
     7921 agaacggtat catcaacttt gacgacatca actcgttccg catcgatatt ctctcgggtg
     7981 aagcttttgt ccgcaccgag atcatggaat acatctttaa taacctggtc ttcaactatg
     8041 acggttcgcc gctcaagaac atgaagatgg agtttttcga cgacgaggac aatggcaagc
     8101 ccatgaagaa gctgcgcctg accggtgaca tgaagctcgt ggtctggctc ggcttcgaga
     8161 tggtcggcaa gatgtacctc gaccgcgaga agacgctgat ggtgatcgaa gccgaacgca
     8221 tcaagtcgct gggcaacccg tacaccaaga ccctgctcga tactgtcggg ctcaacatgg
     8281 aaaaactgct gccggtgcca tcgggccgcg gtatcacgat gatgggcaac aagatcatcg
     8341 tcgaaccatt caagattttc ccaccgccgc aaatcggcgg ctttatcagc gacatgcgtg
     8401 tgcagagtaa cggtttgcag ctgttcttct ccagcaagac caaggtaaac tttccaccgc
     8461 tgccggtaaa ggatgtgaag aactacctgt tcttgtacca gggcgacgtc aagtttggca
     8521 agctcatgat ggtcgatgcg cgcctgcaga tggtagatat gtcgcaaaag aatgacttcg
     8581 acttctacct aaagaaatac cttgtaccgc tgggcgccgg gcattccaag attctgcgcg
     8641 acggttctgt cgttgcgtat attcccgatt accgcgacgt agccaggtag cggtgcgctg
     8701 cgcggtaccg ggcgtcattt taccactgct gcttgctttc gcctgcagca gaaaggctga
     8761 agtactgcct gaaggcgctg cgcgtgtcac acgttctgac agcctggttg aaaacggtaa
     8821 ccagtcttac gagctgcgga gctatacttt tcagctgaaa ctggccgaag gcgaggcttc
     8881 gttcgtctgc gaggcaaaaa aattcacagg cgcggcagag gcgctggcgg ctgcagagaa
     8941 aagtatcgcc cagcggcccg atgaacagca gaggaatagc tccgcctggt atgcgcgcgc
     9001 cggactttcg cagtggttgc tgaccggcag aacgcttacc tggtgcatgc agtctggcag
     9061 cggcagcgct tcgcgcgaag cagcacaggc gaagctgcgc gaggcgtttt tgaagaggtt
     9121 tcccgaggca tgatgatgac aatcttccag cgcatattct cttcgagaat tgtagcaggc
     9181 gctatactgt tggtgctttc gtgcaccagg ccacaggctg aaagccagcg cgtggcggaa
     9241 cccggccagc agttccaggc gtattggaat gccggtaaag ccgagctggc gcgatatgag
     9301 ctgaggcagg cgcgctatgg gcaggtgcac cccggcgaga tggtggtcat caccgtcact
     9361 gaaccttttc gcaccgacag gcaggtgaag gccgagtctg ccgaaggcgc gcagcatgca
     9421 gtgaatgtgt tgaaggtgca gcagatgcgg cgtttcgcga cgggtattta cgactatgca
     9481 ctcaccacaa cgagctttcg acccttcgcc gcgcagccag ccgcagtgct caaggtaacc
     9541 ggcagcgcgg tggactggtg cggccatgcg tggctgcagc tcaaccgcag aaaagacagc
     9601 ttcgccgttg aatcgcgttc gtactttgaa aaccccggcg atatggagta ctcggtttcg
     9661 gcggcgctga gcgaagatga aatctggcag cggattcgta cgaacccggc tgccctaccg
     9721 gtgggaaaaa tcaccgtggt gccttcgctc atgtcggcgc ggctgcgcca ccgggcgaca
     9781 caggtcgaaa cggcgcgcgc agagctgcag cagttcgcgc ggctgaaaca gaccgagtac
     9841 agcctggtct atggacaggg gcaagacgac gagcggcagg tggcctttat cttcgagagt
     9901 gagtttccac accgggtgct cgagtaccgc gagctgtata gcgacgtgat tggtggcggg
     9961 ggaaaaaaag acaagctcag cacagtagcg cggctcaaga agacaatcct cagcccgtac
    10021 tggcagcagc acgacctcat acataagaaa gaccgaaagg actttggagt acgcggattt
    10081 gactaacaag caggttactg aacctgctaa accaaggaga tcactgtgag agtattcatc
    10141 gtaggggccg gtgcagtttc cggcggggca gcgattgccg ctgcaaaatc gcttggcgct
    10201 gagataattg cgacaacaag ccgggcagag aacgttgcgg gcgccgacca caccatctat
    10261 ggcattgacc ttgagcgcag cgatgcggtc gacaagatgc tcgccgacaa aaagctcacg
    10321 gcgaaacccg tcgacttcgt cgtctatatt cccgcgcgcg gcgccgttgg catggatgtc
    10381 accgagtcga ccgccgacat ggtgatgccg agcctcgaat actccgtgat tccgtatctg
    10441 aagctggcga aggcgctgaa gccgaagcgt accattgcgc tctcaggctt catggcgctg
    10501 ccgacgctga ccaaaattta cggcgccatg accttcacga aagtcgcgat ggagcagctc
    10561 gcggtgaata accccgacaa gctgcagatc atccgtatcg gcatgtttct cagtaactcg
    10621 gtgcgcggca tcgcgattct ggcgcagcgc cgtttcatgc gcgaacctga tttccagcct
    10681 gaatggcgcg cgcagtggaa ggcctcgggc aagaagttca gccagttctt ctacgacatg
    10741 aactacgagc aggaagagca gatctaccgc cagcacagca acggcgtacc cttcaggccc
    10801 acgctgcccg aagacatcac caatggtttc ctcatggcgt tcaagggcga acaggcgccg
    10861 atcatcaacg tattaggtcc atggctgtgg acagagcagc aaatgcccga gttgcccgag
    10921 ca
//
LOCUS       DAOQJF010000071        10917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3087_length_10917_cov_4.410145, whole genome shotgun sequence.
ACCESSION   DAOQJF010000071 DAOQJF010000000
VERSION     DAOQJF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3087_length_10917_cov_4.410145"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLF85_05530"
     CDS             complement(<1..257)
                     /locus_tag="PLF85_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNE19155.1"
                     /translation="MQIRTRVSEMLGIDYPIIGAPMFLVSYPDLVCAVSNAGGIGSMP
                     ALNYRTTDELRSGLAEMRARTQKPIGINLILHKEHNPRWAE"
     gene            complement(303..917)
                     /gene="yihA"
                     /locus_tag="PLF85_05535"
     CDS             complement(303..917)
                     /gene="yihA"
                     /locus_tag="PLF85_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNE19156.1"
                     /translation="MVTGKDVFPDSQRIVALLEGKFGEVSALPESQAEIAVIGRSNSG
                     KTSLLKALLNCPNPPQVSSRPGSTRLMHLYRLGQAGSASRATLVDFPGYGYAQSSAVF
                     RQRFSRMLLDYLQSGRPVKAICLIMDCRRETAEEEQEIARIAKERRVPLLLCLNKCDQ
                     LNQSETAKLKMRYQNAEGYFEIILMSAGKRLNLGYIRNYIVSVG"
     gene            complement(926..1381)
                     /locus_tag="PLF85_05540"
     CDS             complement(926..1381)
                     /locus_tag="PLF85_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE19157.1"
                     /translation="MLRSLFKTKSTTPKYRNPVPTVDIIIETPKGIVLIERRNAPHGW
                     AIPGGFVDYGETLEHAARREAKEETSLDVELKTLLYVYSAPSRDPRQHTISAVYIASA
                     KGTPRAADDAKNLRVFKPAELPAELAFDHRKILDDYIKFRKTGRLPKPV"
     gene            complement(1381..1551)
                     /locus_tag="PLF85_05545"
     CDS             complement(1381..1551)
                     /locus_tag="PLF85_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19158.1"
                     /translation="MQQKTAETAEEKKILDLDRYRLKMKLRDSGVEWHEDTKGKFKIW
                     VRLGNQDQVAQH"
     gene            complement(1623..3452)
                     /locus_tag="PLF85_05550"
     CDS             complement(1623..3452)
                     /locus_tag="PLF85_05550"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNE19159.1"
                     /translation="MGYATLHEAVLDLEKTGQLVRVREEIDSNLEMAAVHRRVHSRGG
                     PAIFFENVRGAAFPAVSNLFGTAQRAHFLFRDSFAAVKDVMRLKGTPPAILNPRTLWH
                     ALSIGVHALPHRTLWAAGFKPAQLGDLPVTKSWPRDGGSFVTLPLVYTEHPDKPGVMR
                     SNLGMYRVQTDGNAYEPGKTMGLHYQLHRGIGIHHTRAAELKRPLPVTLIVGGTPALI
                     LSAVMPLPENIPEVLFASMLNRRSFAYRRRNGWLYANEADFLVHGEIHPQETLPEGPF
                     GDHLGYYSLTHDFPVFRIKQILARKKNAIWPFTVVGRPPQEDSQFGALIHELTDGLVS
                     KEIPGVREVHAVDAAGVHPLLLALGSERYLPYAVPQPREILTQANAILGFNQMSLAKY
                     LFITAPHGKDTPGVKDTGAFLKYILERADFRRDLHFQTRTTMDTLDYSGGSLNEGSKL
                     VIAAYGPKQRDLATEVPQKGISRYNFRDVRLAMPGVLAVRTTPFRDYKSSDYEIRSVV
                     SASRALREQGIALIVLVDDAEFASRSLENFLWVTFTRSDPARDIYGVDATVEFKHWGC
                     AGPLIIDARAKHHHAGVLVEDPKVEERINRFFLRGASLAGLDG"
     gene            complement(3452..4195)
                     /locus_tag="PLF85_05555"
     CDS             complement(3452..4195)
                     /locus_tag="PLF85_05555"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNE19160.1"
                     /translation="MEKAQRHISLGINIDHVATLRNARGENDPALIELALLAQNAGAD
                     LITVHLREDRRHIRDADIFDLIKNLKIPLNFEMALSDEILKIALEAKPTTACIVPERR
                     EEITTEGGLDVINHLGKVTQFVQKLHLAGTETYLFIEPDEAVVDAAKKSGAQGIEFHT
                     GAYARSFTNAPRRRHELTRLTRASKHAASLGLEVHAGHGLNYHNVALICAIGEITELN
                     IGHAVIARSLRTGLEAAIRDMRAAMDQAL"
     gene            complement(4202..5197)
                     /locus_tag="PLF85_05560"
     CDS             complement(4202..5197)
                     /locus_tag="PLF85_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNE19161.1"
                     /translation="MILRVLRYIFIEDYLAKLICLAIGSGLWFYVEFARVSQTTLNVP
                     VEYIKKPANLYLKAGQARFVKIVVRGRDEFLKFSTTGIKAEVNLANGQGGERSYPLLF
                     DARQLPERVELASRPDAITVGLEKGMSKVIPVRVSTTGNPDAQFRLLRATATPQQIEI
                     DGPEAAVAAIQAIETDAVDIEGARANIQRKVNLRIPDQVATDKLKNVVVRVELIQKTY
                     SEEQQFEQIPLKVQNLDAALNAALSDNVVSVLVQGESGAVKKLKSSDFYAWVNAEDTR
                     YNSRTGNILPYANESGMPVKARILSGNKKVQIVAITPDKVNVRFSVKPEYAKKTD"
     gene            complement(5194..5955)
                     /gene="cdaA"
                     /locus_tag="PLF85_05565"
     CDS             complement(5194..5955)
                     /gene="cdaA"
                     /locus_tag="PLF85_05565"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNE19162.1"
                     /translation="MNFSPRDLLDILLVTLVVYYGYRLVRQSRAVPILGGVAIFLALT
                     FISHRAQLETLSWLFDSISAYFVIGILVVLQPELRRLFYQIGQARWYRTLIQVQQVPV
                     DEITSACKQISENKFGALIAIVNKVGLRQYSESGVQLGGRVSRELLNSIFFGKNPLHD
                     GAVIIEGQNIVAAACYLPMSASNEIKRSYGARHRAALGLSEDSDALVIVVSETNGKIA
                     LAFLGEVKENIDQARLRKILIAFNQNQLSEEWKAL"
     gene            complement(5945..>6781)
                     /gene="folP"
                     /locus_tag="PLF85_05570"
     CDS             complement(5945..>6781)
                     /gene="folP"
                     /locus_tag="PLF85_05570"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNE19163.1"
                     /translation="RGASVHRENPGPPVLWAVINLTPDSFYAGSRATVGEAIARAEKM
                     FADGAAVIDVGAESARPGASPVSAAAQSEVLLSFLDKYRSHFGDTFMRRISVDTRELP
                     VMQQVAEWGVGYINDVSGGSPEIYRFIAESGLEYVLMHTQGNPQEMQKAPTYNDVCAE
                     VLAYLQLRTALLKESGVKAEKIIWDTGIGFGKTVEHNLALIAAHARFREFGYRVLAGV
                     SRKSFIGRVLGQDDAADRLVGTLTTQIYLTLHGLEILRVHDVKEMADALRMIAAIKQH
                     EL"
     assembly_gap    6782..6881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7073..8119)
                     /locus_tag="PLF85_05575"
     CDS             complement(7073..8119)
                     /locus_tag="PLF85_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19164.1"
                     /translation="MRKYNILAISLLAAPGLFARTSSATFLTEGFGAKSVGLGNAYGV
                     VVSDPSAVYWNPAGLAQIYAEKRTTQTIETPKSAEKPAGDDEEFNKLLEDQNTAGSKE
                     SKTVEITERGYQMQLNSSASYLSNGRLMYFGAAGVTLGSGAIGVGYGGTRIASINGYD
                     SAGNATGANSYYGHTAYLGYGWGTGPVRLGISGFAVREDLAGGAVNGGGINVGAQVVV
                     LPPLLSVAADIKNLAAIQNRTGGAFRELGKIDTLFSFSMQVQAPPPNSNFKLIIGFTA
                     NLDKPQGDGVRLNMGMAYGFNKYMYAMLGLNGSKPAVGLGVNVGKTFQMAFSANRDTM
                     GREFQYFGELNFAF"
     gene            complement(8128..8514)
                     /locus_tag="PLF85_05580"
     CDS             complement(8128..8514)
                     /locus_tag="PLF85_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19165.1"
                     /translation="MKKIVLISLALVAFTSACNNMVPDSTQALKAYPNPYNPTAGVLT
                     IENVDGSDFSTSQNDFIVYDFALNEIYRANLLPVDAPTSKKKLVWAGIDNAAVKVSPG
                     IYYIKVVTTTNTGALNVDSMFKLIVQ"
     gene            complement(8533..8889)
                     /locus_tag="PLF85_05585"
     CDS             complement(8533..8889)
                     /locus_tag="PLF85_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19166.1"
                     /translation="MAEKLIYDAKEPLWIAGNKPLTEQFLNQFYSTINPAQLHRLEKL
                     ARALHGEIEIINMTPQQFVSQVNASYQRYQQHRGLDSFHPMDDKSLKYVLEFMEESIK
                     EVAKEMAKNSAKSLIR"
     gene            complement(8927..>10917)
                     /locus_tag="PLF85_05590"
     CDS             complement(8927..>10917)
                     /locus_tag="PLF85_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE19167.1"
                     /translation="GFARLRNRSLMGSMHTALEETEKGFERLAAFYAERARGEAGIIV
                     TGGIAPNPAGRVFTGAAKMDTEHEADEHRVITKAVHDNGGLIAMQVLHTGRYGYHRDC
                     VGASEIQAPINFVKPKKLSHEDILKTIADYAQCAKLAQYAGYDGVEIMGSEGYLINQF
                     TAARTNNRDDEWGGTYEKRMRFPIEVVKAVRKAVGEKFIIIYRLSLLDLVDGGSSWEE
                     VVQLGKEIEKAGATIINTGIGWHEARIPTIAMVVPRGAFAGVTGKLKKEVKLPVITSN
                     RINTPELAEAIIARGDADIVSMARPFLADGEFVAKARAGKSHLINTCIACNQACLDHT
                     FGGKLTSCLVNPRACNETNLNYVKTTAPKKVAVVGAGPAGLSVATVLAQRGHAVTLFD
                     AAQSVGGQLNIAKQIPSKKEFDETIRYFGNMLQEHKVEVRLGKKVSADELVAGGFNEV
                     VLATGIVPRKPPIPGADHPKVLSYIDVLRDKKPVGNKVAIIGGGGIGYDTALYITDPG
                     HATWQKPDEFAKEWGFDLSVSVRAGLTTKAPEYLQSPREVTMFKRSKGKFGNTLGKTT
                     GWVHKITLEERRVQQVSNVTYDKIDDEGLHYTLKGEQKVLPCDTVILCAGQEPLRELE
                     SGLAAKGMPVHIIGGAKEAGELDAKRAIAEGAELGARL"
BASE COUNT         2215 a         3059 c         2984 g         2559 t
ORIGIN      
        1 tgttctgccc agcgcgggtt gtgctctttg tgcagaatca ggttgatacc gatcggcttc
       61 tgtgtgcggg cgcgcatttc tgccaggccg ctgcggagtt catcagtcgt acggtagttg
      121 agcgctggca ttgagccaat gccgccggca ttagaaactg cgcagaccag gtcgggataa
      181 gacaccagga acatcggcgc accgataatc gggtaatcga tacccagcat ttcactgacg
      241 cgggttctga tctgcatgta taagacttgg tcttcttgtc agggcgtaaa tgatatcgcc
      301 gcttagccga cagacacgat gtaattgcgg atatacccga ggttcaggcg tttgcccgcc
      361 gacatgagaa taatctcaaa gtaaccctcc gcattctggt agcgcatctt cagcttcgct
      421 gtttcagact ggttcagctg gtcacatttg ttcaggcaaa gcagcagcgg tacgcggcgt
      481 tctttggcga tgcgggcaat ttcctgctct tcttcggcag tttcacgccg gcagtccatg
      541 atcaggcaga ttgccttcac cggcctgcct gactgcaggt aatcgagcag catgcgcgag
      601 aaacgctggc ggaatacggc agaactctgt gcataaccgt agcccggaaa atcgaccagt
      661 gttgcccgtg aagcagaacc cgcctggcca agccgataga ggtgcatcag ccgcgtactg
      721 ccaggccgtg aagacacctg cggaggattc ggacagttga gaagagcctt gagcagcgag
      781 gtcttgccgc tgttcgaacg gccgatcacg gctatttcgg cctgcgattc aggtaatgcg
      841 ctcacttcgc cgaatttgcc ctcgagtaag gcaacaatac gctgtgagtc aggaaatacg
      901 tctttgcccg taaccatgcc gcctgtcaga ccggctttgg cagccggcct gtcttgcgga
      961 atttgatata gtcgtcgaga attttccgat ggtcaaaagc cagttcggcc ggcagttcgg
     1021 cgggtttgaa gacccgaagg tttttggcat cgtcggctgc gcgcggcgta ccttttgcgc
     1081 tggctatata gacagcagag atcgtgtgtt gccgtgggtc gcgcgaaggt gcagaataga
     1141 catacagcag ggttttgagt tcaacatcga gactggtttc ttcttttgct tcgcggcgtg
     1201 cggcatgttc gagggtttcg ccgtagtcga caaaaccgcc agggattgcc cagccatgcg
     1261 gcgcatttcg ccgttcgatg agtacaattc ctttcggagt ctcaataata atatcaaccg
     1321 tcggtaccgg gttgcggtac tttggggtgg tggacttagt cttaaagagc gaacgcagca
     1381 tcagtgctgt gccacttggt cctggttacc gagccgcacc cagattttga atttgccctt
     1441 cgtgtcttcg tgccactcaa cgcccgagtc gcgcaatttc attttcaggc ggtacctgtc
     1501 gagatcgagt attttcttct cttccgctgt ctctgctgtt ttttgctgca ttcgatgccc
     1561 cttgaagagt atcgggcagc aggcgggtca aaattgaaga tccgtgcggg cgttatgtcg
     1621 cttcagccat ccagcccggc gagcgatgcg ccgcgcagaa agaagcggtt gatcctttct
     1681 tcaaccttcg ggtcttctac cagtacaccg gcatggtggt gcttcgcgcg cgcatcgatg
     1741 atgagtggcc ccgcgcaacc ccagtgtttg aactctaccg tggcgtcgac gccatagata
     1801 tcgcgcgccg ggtctgaacg cgtgaaagtt acccagagaa agttctcaag actccgcgag
     1861 gcaaattctg catcgtctac caggactatc agcgcgatac cctgttcgcg cagggcgcgc
     1921 gaagccgaaa ccacagagcg tatttcatag tcggagctct tgtagtcacg aaacggcgtc
     1981 gtgcgtactg ccaacacacc cggcatcgcg aggcggacat ccctgaagtt gtagcgggaa
     2041 atacctttct gagggacttc agttgccagg tcacgctgct tgggcccata cgctgctatc
     2101 accagctttg aaccttcatt cagcgaaccg cctgaatagt cgagcgtgtc catcgtcgtg
     2161 cgtgtctgga aatgcaggtc acgccggaaa tcggcgcgtt cgagtatata tttcagaaac
     2221 gcaccggtgt cttttacacc gggcgtatct ttgccatgcg gtgcggtgat aaagaggtac
     2281 ttggcgagcg acatctggtt aaagccgaga atcgcatttg cctgcgtcaa aatctcgcgc
     2341 ggttgcggta cagcatacgg caagtagcgt tctgagccga gcgcgagtaa aagcgggtga
     2401 actcccgcag cgtcgacggc atgcacttcg cgcaccccgg gaatctcttt tgacacaagg
     2461 ccgtctgtca gttcatggat cagcgcgcca aactgcgaat cttcctgcgg tggccggccg
     2521 acgacagtaa aaggccagat agcatttttt ttccgcgcca gaatctgttt gatgcggaag
     2581 accgggaaat cgtgcgtcag gctgtaatag cccaaatgat cgccaaacgg cccctcgggc
     2641 aaggtctcct gcgggtgaat ttcaccgtga accaggaaat cggcttcgtt ggcatataac
     2701 caaccattgc gcctgcggta ggcaaaactc ctgcggttca gcatcgacgc gaaaaggact
     2761 tcgggaatat tctccggcag cggcatgaca gccgaaagga tcagcgcggg tgtgccgccg
     2821 acaatcaggg tcacaggcag aggacgcttg agctccgccg cgcgcgtatg gtgaataccg
     2881 ataccccggt gcagctggta gtgcagcccc atggtcttgc ccggttcgta cgcgttgccg
     2941 tcagtctgta cgcggtacat gcccaggttc gagcgcatta cgcccggctt atccgggtgc
     3001 tcggtataaa ccagcggcag agtcacgaaa ctgcctccgt cacgcggcca cgatttcgtc
     3061 accggaagat caccaagctg cgcgggcttg aaacctgctg cccaaagcgt gcggtgcggt
     3121 agcgcgtgca caccaatgga cagcgcatgc cagagcgtcc ttgggttcag aatggccggc
     3181 ggagtccctt tgaggcgcat gacatctttg accgcggcaa aagaatcacg gaacaggaaa
     3241 tgcgcccgtt gggctgtgcc aaacaggttt gagaccgcag gaaatgctgc gccccggaca
     3301 ttttcaaaga agattgccgg accaccccgt gaatgcacac gccggtggac ggctgccatt
     3361 tcgaggttgc tgtcgatttc ttcgcgcacg cgcacgagct gcccggtctt ttcgagatcg
     3421 agcactgcct cgtgcagcgt ggcgtaaccc atcagagagc ctggtccatc gccgcgcgca
     3481 tgtcgcggat tgccgcttcg agccccgtgc gcaggctgcg tgcaatgacc gcatggccaa
     3541 tattcagttc ggtgatttca cctattgcac aaatgagcgc cacattatga tagttaagcc
     3601 cgtgccctgc atggacttcg agcccgaggc tcgcggcgtg tttggaagca cgggtaagac
     3661 gcgtgagctc gtgccggcgg cggggcgcgt tggtaaaaga gcgcgcatag gccccggtat
     3721 ggaattcaat gccctgcgcg ccggattttt tagcggcatc aacaacagct tcatcgggtt
     3781 caataaagag gtacgtttct gtaccggcaa ggtgcagctt ctgcacaaac tgggttacct
     3841 tgccaaggtg atttatgacg tcgagcccac cttcggtggt gatttcttcg cgcctttcgg
     3901 gcacgatgca ggctgttgtg ggttttgcct cgagtgcaat tttcagtatt tcatctgaaa
     3961 gtgccatttc gaaattgagg ggaattttca ggttcttaat caggtcaaaa atatcggcat
     4021 cacggatatg cctgcggtct tcgcgcaggt gtaccgtaat caggtcggcc ccggcattct
     4081 gcgcgagcag cgccagttca atcagcgcgg gatcgttctc gccgcgtgca ttgcgcaggg
     4141 tggcaacgtg atcgatattg attccgaggc tgatgtgtct ctgtgctttt tccattcttc
     4201 ctcagtctgt ttttttggca tattcgggtt taaccgaaaa gcgcacattc actttgtccg
     4261 gtgtaatggc gactatctgt acttttttgt tcccgctcag gatgcgggcc ttgaccggca
     4321 tgcctgactc attggcatac ggcagtatat ttccggtgcg cgagttgtac ctggtatctt
     4381 ctgcgttcac ccaggcgtaa aaatcggagc tcttcagttt tttcacagcg cccgactcgc
     4441 cctgaacgag cacagaaacc acgttgtctg aaagcgccgc gtttaatgct gcatcgaggt
     4501 tctgcacttt gagcggaatc tgttcgaatt gctgttcttc gctgtaggtc ttctggataa
     4561 gctccacccg gacgaccacg ttcttcagct tgtctgtcgc gacctggtcg ggtatgcgca
     4621 ggttgacttt gcgctggata tttgcccgcg caccctcgat atccaccgca tcggtctcaa
     4681 tcgcctggat agccgccaca gcagcttcgg gaccatcgat ctcgatttgt tgcggtgtcg
     4741 ccgttgcgcg caacaacctg aactgtgcat cgggatttcc cgtggtggaa acacgcaccg
     4801 gaatgacttt gctcatgcct ttttcgagac cgacagtaat tgcatccggt cggcttgcga
     4861 gttcaacgcg ctccggtagt tgccgggcat cgaagagcag cggataactg cgttcaccac
     4921 cctgcccatt ggcaagattc acttcagcct taatacctgt ggtggaaaac ttgagaaact
     4981 catcgcgccc gcgcacgaca attttcacaa accgggcctg gcccgctttc aaatacaggt
     5041 ttgcaggctt cttaatatat tcaacgggaa cgttcagcgt cgtctgagag acgcgcgcaa
     5101 actcgacgta aaaccagagc cctgacccaa tagcaaggca gatcagcttt gcgagataat
     5161 cttcaatgaa tatatagcgc agtacccgga gaatcacagg gccttccatt cttcactcag
     5221 ctggttctgg ttgaaggcaa tcagtatctt gcgcagtcgc gcctgatcga tattttcctt
     5281 tacttcgccg agaaaagcaa gcgcgatctt cccgttggtt tccgatacga cgatcacaag
     5341 cgcgtcggag tcttcgctca agcccagcgc cgcacggtgc cgcgcgccgt acgaacgctt
     5401 gatctcgttt gaagcgctca tcggcaggta acaggcagcg gccacgatgt tctgtccttc
     5461 gatgatgacc gcgccgtcgt gaagcgggtt cttaccgaaa aagatcgagt tgagcagttc
     5521 gcgcgaaacc cggccaccca actgcacgcc cgactcgctg tactgccgca ggccgacctt
     5581 gttgacaatc gcgatcagcg caccaaactt attttcgctg atctgtttgc atgcactcgt
     5641 gatttcatcc acaggaacct gctgcacctg tatcagcgtg cggtaccagc gcgcctggcc
     5701 aatctggtag aagagtcggc ggagttccgg ctgcaggaca accaggatgc cgatgacaaa
     5761 gtacgccgaa attgaatcaa agagccagct caatgtctcg agctgtgcac ggtggctgat
     5821 gaaggtgagc gcgagaaaga ttgccacgcc acccaaaatg ggaactgccc tgctctgccg
     5881 caccaggcgg tagccgtaat aaactaccag ggttaccagc agtatatcga gcaggtcgcg
     5941 cgggctaaag ttcatgctgt ttgatggccg ctatcatgcg cagcgcgtct gccatttcct
     6001 tcacgtcgtg aaccctcaga atttcgaggc catgtaaagt taaataaatc tgcgtcgtga
     6061 gcgtacccac cagccggtct gccgcgtcgt cctggcccag tacacgcccg ataaatgact
     6121 tgcgcgatac gcccgcgagt accctgtacc cgaattccct gaaccgggcg tgtgccgcaa
     6181 tgagcgcaag attatgttct acagtcttac cgaagccgat gccagtgtcc cagataatct
     6241 tctctgcttt gactcctgat tctttgagca aggccgtgcg cagctgcagg taagccaaaa
     6301 cttcggcgca gacatcgttg taggttggtg ctttctgcat ttcctgcggg ttgccctgcg
     6361 tgtgcatgag cacatattcg aggcctgact ctgcgataaa gcggtagatt tccggcgaac
     6421 ctccactcac gtcgttgatg tagccgacac cccattcggc cacctgctgc atgaccggca
     6481 attcccgtgt atcgaccgaa atccgccgca tgaaggtatc gccaaagtgg gagcgatact
     6541 tgtccagaaa gcttaacagt acttcagact gcgccgccgc gctgacgggg ctggctcccg
     6601 gtcgcgcgct ttccgcaccg acatcgatga ctgcagcgcc gtcggcgaac atcttttccg
     6661 cgcgcgcgat ggcttcgccc accgtcgcac gcgaacccgc ataaaatgaa tcgggcgtca
     6721 gattgattac tgcccaaagg acaggaggtc ctgggttctc acggtgcacg gatgcacctc
     6781 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggttctcac ggtgcacgga
     6901 tgcacctctg aatcagccgc atatcggatt tataccgcat atccaccgtg gccctgctca
     6961 gctggcccgg aaaccagagg cgctacggtt tgcgatgcaa acctgccgca aaacggccct
     7021 ctgggcctgc ctccggtttc ctctcctgac ggaacgccag ctggcagaag ctttagaatg
     7081 caaagttcag ttcgccgaaa tactggaact cgcgccccat ggtatcgcgg tttgccgaga
     7141 acgccatctg gaaagtttta ccgacgttca cgccgagacc aacggctggc ttcgagccat
     7201 tcagaccgag catggcgtac atgtacttgt tgaaaccata tgccataccc atgttgagcc
     7261 gcacgccgtc accctgcggt ttgtcgaggt tcgcagtaaa accgatgatc agtttgaagt
     7321 tcgagttggg tggcggtgcc tgcacctgca tagaaaagct gaaaagcgta tcgattttcc
     7381 ccagctcacg gaatgccccg ccagtacggt tttgtatcgc agcaaggttt ttgatatcag
     7441 ctgcgaccga gagcaagggc ggcaacacga cgacctgtgc accgacattg ataccaccgc
     7501 cgttcaccgc gcctcctgca aggtcttcgc gtacggcaaa gccgctgata ccgagacgta
     7561 ccgggcctgt accccagccg taacccaggt aggcggtatg gccgtaatag ctgttggcgc
     7621 cagttgcatt gcccgcggaa tcataaccgt tgatgctcgc gatgcgcgtg ccgccatagc
     7681 ctacgccaat ggcacctgaa ccgagggtga ctcctgcagc gccgaagtac atcaggcgtc
     7741 cgttggaaag ataactcgcc gatgaattca actgcatctg gtaaccgcgt tcggtgattt
     7801 caacggtctt cgattctttg ctgcccgcag tattctggtc ttcaagcagc ttgttgaatt
     7861 cttcgtcgtc accagccggc ttctctgccg atttgggggt ttcaatcgtc tgtgtcgtgc
     7921 gtttctcggc ataaatctgc gccagacccg ccgggttcca gtacacggct gacggatcgg
     7981 agacgaccac gccgtaggca ttaccgaggc cgacgctctt ggcgccaaaa ccttctgtca
     8041 ggaaagtcgc cgacgaagtg cgcgcgaaaa gcccgggcgc tgccagcagg gagattgcga
     8101 ggatgttata tttacgcatg gtttacctta ttgtacaatc agcttgaaca tgctatcgac
     8161 gttcagtgcg ccagtgtttg tcgtggtcac aactttaata tagtagatgc cgggtgatac
     8221 tttgactgct gcgttatcga tgccagccca aacgagtttc ttttttgagg ttggtgcgtc
     8281 gactggcagc aggttggcac gataaatttc gttgagtgcg aaatcataga caatgaaatc
     8341 attctgtgaa gtgctaaaat cacttccatc gacattttca attgtaagca ctccggctgt
     8401 cgggttgtag gggtttgggt acgccttcag cgcctgcgtg ctgtcgggca ccatgttgtt
     8461 gcaggccgag gtgaacgcca ccagggcaag gctaataaga actatttttt tcattgggct
     8521 cctttgagaa atttagcgaa tcaggctctt ggccgaattc ttcgccattt ctttggcgac
     8581 ttctttgatc gactcttcca tgaactccag tacgtacttg aggctcttgt cgtccatcgg
     8641 gtgaaaactg tccagccccc ggtgctgctg gtaacgctgg tacgacgcat ttacctggct
     8701 cacgaactgc tgcggggtca tgttaatgat ttcaatttcg ccgtgcagcg ctcgcgccag
     8761 cttttcaagg cggtgcagct gtgccgggtt gatggtgctg taaaactggt tgagaaactg
     8821 ctcagtgagc ggcttgttgc ctgcaatcca caaaggttct tttgcgtcat atatcagttt
     8881 ctcggccatg aagtactccc cttactttaa gtatcggctg ctgcgtctac aaccttgcgc
     8941 ccagttctgc cccttcggcg atggcccgtt tcgcatcgag ctcccccgct tctttggccc
     9001 caccgataat gtgtaccggc atacccttgg cagccaggcc cgattcgagt tcacgcagcg
     9061 gctcctgacc ggcgcagaga atcacagtat cgcacggcag aactttttgc tctcccttga
     9121 gcgtatagtg cagaccctcg tcgtcgatct tatcataggt gacgttagag acctgctgga
     9181 cacgacgctc ttcgagcgta atcttgtgca cccagcctgt ggttttgccg agcgtattgc
     9241 caaacttgcc cttgctgcgc ttgaacatgg ttacttcgcg cggtgactgc aggtattcgg
     9301 gagcttttgt ggtaaggccg gcacgcaccg aaacggagag gtcaaagccc cactctttcg
     9361 caaattcgtc gggtttttgc caggttgcat gacccgggtc ggtgatgtag agcgcagtat
     9421 cgtaaccgat accgccaccg ccgataatgg ctaccttgtt gcccactggc ttcttgtcgc
     9481 gcagcacatc gatgtagctc agaaccttcg ggtggtcggc tccaggaatc ggcggcttac
     9541 gcggcacgat acccgttgca agtacaactt cgttaaatcc gccagctacg agctcatcgg
     9601 cgctgacctt cttgccgagc cgcacctcaa ccttgtgctc ctgtagcata ttgccaaaat
     9661 atcggatggt ttcatcgaac tctttcttcg acggtatctg tttggcgata ttcagctgcc
     9721 caccaacgct ctgtgcggcg tcaaacagcg tcacagcgtg gccgcgttgc gcgagtacag
     9781 tcgcgaccga aagcccggcg gggcctgcgc cgacgacggc cactttcttc ggcgcagttg
     9841 tcttcacgta gttcaggttc gtctcgttgc acgcgcgcgg gttcacaagg caactcgtca
     9901 gcttaccgcc aaaggtatgg tcgaggcatg cctggttgca ggcaatacag gtattgatca
     9961 ggtgtgactt gccagcacgc gctttcgcga cgaactcgcc gtcggcgaga aacggtcgcg
    10021 ccatcgaaac gatgtctgcg tcgccgcggg cgatgatcgc ctcggccagc tcaggtgtat
    10081 tgatgcggtt cgaggtaata acggggagtt tcacctcttt ctttagtttg cccgtgacgc
    10141 cggcaaaggc cccgcgaggt actaccatcg cgattgtggg aattcgtgct tcgtgccagc
    10201 caattcccgt gttgatgata gtggcaccgg ccttctcgat ttctttgccg agctgaacca
    10261 cttcttccca gcttgagccg ccatcgacca ggtcgagcag cgagagacgg tagatgatga
    10321 tgaacttttc gccaactgcc ttgcgcaccg ctttcaccac ttcgatggga aaacgcatgc
    10381 gcttttcgta cgtaccgccc cactcgtcgt cgcggttatt ggtacgcgcc gcggtgaact
    10441 ggttgatcag gtaaccttcg ctgcccatga tctcaacgcc gtcgtaaccg gcatactgtg
    10501 ccagctttgc acactgcgca taatcagcaa ttgtcttgag aatatcttcg tggctgagtt
    10561 ttttgggttt tacgaagttg attggcgcct gaatttccga tgctcccacg cagtcgcggt
    10621 ggtagccgta gcgcccggta tgcagcacct gcatcgcaat caatcctccg ttatcatgca
    10681 cagccttggt gatcacgcgg tgttcgtcgg cctcgtgctc ggtatccatc tttgcagcgc
    10741 cggtaaagac acgtcccgca ggattcggcg caataccgcc cgtgacgatg atgcctgcct
    10801 caccgcgcgc acgctccgca tagaatgccg ccagtcgctc gaagcctttt tcggtttctt
    10861 cgagcgccgt gtgcatcgag cccatgagcg aacggttacg caacctggca aagccca
//
LOCUS       DAOQJF010000072        10908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3091_length_10908_cov_4.772229, whole genome shotgun sequence.
ACCESSION   DAOQJF010000072 DAOQJF010000000
VERSION     DAOQJF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_10908_cov_4.772229"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="PLF85_05595"
     CDS             complement(<1..1499)
                     /locus_tag="PLF85_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNE19168.1"
                     /translation="MHARKIACLFVLSFIASCRGFNWPKPIKPGAETQLTQVFSVVTT
                     NPADTDALVANNTQLVLNFSETVNTGSLTYNSAAGPCGTNNLQVSADDFITCIALTTP
                     VWGSSQTSLTMTPLATLANGTTYKIRVLTTLMAVSGRVLSSAFTTTTGFTTAPPGTLV
                     PSMASPSEGATAVSVNTALMINFTHPVSTGTLSYNSTAGPCTGSFQMSADGFVNCIAF
                     PSHVFMVADTQLNIAPAVALNYSTLYRVRVTTVLTGAGSETLSGDFTSVGFTTDAPPA
                     LTVTLTTPADGSTGVSPSTTFLLAFNHAVNIGTVSYNNVAGACTGTVQISADGFSNCV
                     GVNAPVWGGGNTSLTLTPGSALNPGALYRVKVTTAVTGAVGNLLASDYITPSGVGILI
                     SAPGSLQVFASNAQNVLIWPTVSGALSYNIYFSTSAGVTTGTGTLIAGVTAPYVHTGL
                     TNGTSYFYIVAGANGGNVGIASAQSSAIPIAFKTIFVSSGNYTGALGGHA"
     gene            1711..2511
                     /gene="flgG"
                     /locus_tag="PLF85_05600"
     CDS             1711..2511
                     /gene="flgG"
                     /locus_tag="PLF85_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNE19169.1"
                     /translation="MVRSLWTSATGMIAQQFHMDTISHNLSNVNTTGYKKNRVDFEDL
                     LYQHQVLAGTPSTAVSEIPVGVNVGVGVRPAATQKLFQMGSLQSTGHKLDMAISSDVG
                     FFKILMPDGTFGYQRNGDFKIDSRRQVLTSDGYTLEPPLVIPPDGLIETLTINEQGEV
                     RIQIGDDQIPRQIGQIELYRFVNPAGLKAIGKNLYKESPASGPEIPGTPGLGGFGGIL
                     QGFLEMSNVNLAEEMVNMIVAQRAYEANSKSIQTSDSMLSTAIQLKRG"
     gene            2604..3113
                     /locus_tag="PLF85_05605"
     CDS             2604..3113
                     /locus_tag="PLF85_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HNE19170.1"
                     /translation="MGILCMSFSMAHAENKTQLALKSTYAAAKSSICLSDIIEAHNLA
                     QREAEKLSRYCRIEFKGARMTLNAKEIELHAWAAGVTPDKISGGPVTISMATTLQLPA
                     NKTAAPVKLRRGSAVQLVLKSEHMQIAREAALLQDAFAGETVDVRPAGTRKTLRAKLV
                     SNTVAELVQ"
     gene            3110..3757
                     /locus_tag="PLF85_05610"
     CDS             3110..3757
                     /locus_tag="PLF85_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNE19171.1"
                     /translation="MSGFVKAAFALVAVSAAINAQESLWRDQNPYVSPPRDGEIVAIE
                     VNETFNIVSDGQWKSSQQFEMKLVPDTKNIPFLTSSQQNKSRSKSSNSNNKTRDSYRF
                     HLTGILGAKQPDGNYPINAQKSLNVDGKPVNVRLTGIIDPRRIRQGTIESRFVGNLSL
                     TVQSEPPFPKDQKLDLKPPAGTDPNSKPTMTEFSDQLRKELLIEHMKQILGGMNQ"
     gene            3754..4851
                     /locus_tag="PLF85_05615"
     CDS             3754..4851
                     /locus_tag="PLF85_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNE19172.1"
                     /translation="MRSAIAILIAAASAASVQALNLTVRDITRISWHRENQVSGYGVV
                     IGLNGSGDSRSELALETLKKNLYNRGIDLGEKTLQAKNIAAVMVTAMIPTHARPGDPV
                     DVWVSSIGDARALNGGMLLQTALTAADGQVYAVAQGPIAAPLPRPTENNNLQLYNFSV
                     YNRRIPPNDRLNQERHNSAHIPGGGIIERGVSQPTVLVDDTKKTKSSKLSLHYFDFVT
                     ARNVVAAINKKLPDTASLANDGTISLNIPYASDHVDYLSKILAVKVDVPERTKVVLDT
                     RTGTIVSGGSVAISAVMVTHNGMQIEVSGKKAYAYGDEKSPANAELKEGATVKELVDN
                     LNKLGLTSAEIIDVIKAIHAAGALHGELTVL"
     gene            4848..5279
                     /locus_tag="PLF85_05620"
     CDS             4848..5279
                     /locus_tag="PLF85_05620"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNE19173.1"
                     /translation="MNVNAINPLLLPQKETPRVGKTDKESAVSFQQLLNDLVPKNLDA
                     KVSSENFSRTPQREKILEHVGASPERKKMFEAARQFENFFLEKMFREMRKNMGKNDIL
                     HGGSAEDIFQDMLLTERVNSLTQSSTLGLAEQIYTQMQRTA"
     gene            5295..6140
                     /locus_tag="PLF85_05625"
     CDS             5295..6140
                     /locus_tag="PLF85_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19174.1"
                     /translation="MFRTKPKPLCPGPTKFFFIVLAFSSLFPLHAQGETGNVGRNETQ
                     DAGTAEAPPASSARPAAGSATQPKRPVKSAALKRAEKLYFSRKYETAAALLRKITDEE
                     PENGRANSLLGDVYYLQGQYDKALSHLLRAAELNDQKAPDYFRLGQTYEKLNKPKEAV
                     RAYQKAYESDEAMKEALFQIGFVYLSLERNKKKTIEFWQRFVDEAVTDPQREKVITVL
                     KFLNDPNFVLPDKNSPISLEEALMLGGGAGNGEVKTGEDKAAGHEKEKEKNDSKELLD
                     DDKLN"
     gene            6151..6516
                     /locus_tag="PLF85_05630"
     CDS             6151..6516
                     /locus_tag="PLF85_05630"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE19175.1"
                     /translation="MPLSNDRRSQHRVTAGAYIEYRVTIDGTMVKDAIMSDISEGGLM
                     VLAESADIGRELNKNEMISGEIIHDRTRFHFKFAGELAWIQPREMRTRKFVALGIRFT
                     PDVQLPAQIYAILMADHDF"
     gene            6536..7429
                     /locus_tag="PLF85_05635"
     CDS             6536..7429
                     /locus_tag="PLF85_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE19176.1"
                     /translation="MKTIILRAGKAIAGWVTVLCLIFLLTVSLQHLRKGSAEEVLMGQ
                     RGTTQSLAALKDKSFGSRLTELAVALPGMVQWKTLRGTMVLPQIGHALAATAMLSALS
                     LLFSVVIGLVMLSAAEFYPRFANPLASLATAINTTPIFLVGIFFIWLFAFALRLLPSG
                     GDGGLRFFVLPALAIAAKFGARLFLLLRNYMNELENRVFILRARAFGFNDRKIFLHKL
                     ANCAMPFIIFWLIETASLFSGAVIVESLFSIHGLGQLLLFALLQYDIRLIFANLVVIA
                     TIVYLTSLLQKVIAETQERSA"
     gene            7426..8193
                     /locus_tag="PLF85_05640"
     CDS             7426..8193
                     /locus_tag="PLF85_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19177.1"
                     /translation="MSDRKMAWLSIAVLVAILLAGILAATPTINLEKTFRAPGSEFWL
                     GSTPLGQSVAQLVLTGAVSTVLLSAGALVLALSAAFGFTGLFYLLPQPVRKVYAGFID
                     AWLAIPGIFIALSIGYFLPQSFLSVMTALVLSEFAPLQKFLLQRLSGIGQADYITMAA
                     VMGAPAVHRLRWHVMPRLAREAGFLFIVTLPSIALSLASLEFLGVQTGSERMSLGLQI
                     AIYKDYIWLYPHLSLAPVVVLLMTLYCLGSASRLLKQ"
     gene            8546..9592
                     /locus_tag="PLF85_05645"
     CDS             8546..9592
                     /locus_tag="PLF85_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNE19178.1"
                     /translation="MKSVACKAGQANSPVASGLFSIDLTPPAAASSFTASAGDSTALL
                     SWVNPGDADFSRAKLVRKAPTPPANSADGTVIYEGTGSAFADLGLANGTTYYYAVFAY
                     DLAGHESAAATASASPLGGLLNAPTYVPAAGTYNLPLSATISSANTGDIICYTTNGST
                     PACDATPTCTTGALASGSVTVAASQTLKAISCKTGSPVSAVAPAAYTIDMTAPVFSGL
                     LPANGASVSSAQLSYSLSETCVTGSVTWTRTGGSADAGSPHTSALTMSELLAGSQSAI
                     TLVNTPPLVQGAIYDIGFSCSDAAGNNSPPALITDVIYDASPPAALSAFRAIAGDGRI
                     TLVWNNPGTWIWRV"
     gene            9788..10732
                     /gene="deoC"
                     /locus_tag="PLF85_05650"
     CDS             9788..10732
                     /gene="deoC"
                     /locus_tag="PLF85_05650"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNE19179.1"
                     /translation="MKSPVPAARPVTQRIYASPTLDPMYTPVDEVAVVERVARFKTRS
                     IKNEAKLAGIKLAISMIDLTTLEGKDSPGKVIQLCQKALRPSELPGVPQVAAVCVFPN
                     MIPIAKKVLKGSQVKIACVSTAFPAAQVPLDIKIEDVRRSVELGADEIDMVISRGQFL
                     AGEYNYVFDEIAQVKEACGKARLKVILETGELETLDNVRRASFIAMRAGADFIKTSTG
                     KVTVNATMPVTLVMLECIRDYYDDTGIQIGMKPAGGIRTSKDALHYLVMVKETLGDAW
                     LDPYWFRFGASSVLNDLLMQYAKQVKGGYQSIDYFSLS"
BASE COUNT         2560 a         3113 c         2906 g         2329 t
ORIGIN      
        1 gcatggcccc cgagggcacc ggtgtagttt ccgctcgaga caaaaatcgt cttgaaagca
       61 attggtatcg ccgaactctg cgctgatgcg ataccgacat tgccgccatt cgcgccggcg
      121 acaatataaa aatatgaggt gccgttcgtc agtcccgtgt gcacataggg tgctgtgaca
      181 cccgcaatca gagtgccagt accggtcgtc acgcctgccg aggtactaaa gtagatatta
      241 tagcttaaag cgccggaaac tgtcggccaa ataagcacat tctgcgcatt cgaggcaaaa
      301 acctgcagac tacctggcgc agaaatcaga atgccgacgc ccgatggcgt aatataatct
      361 gaggcaagca ggttgccgac tgcgccggtc accgctgtcg ttacctttac tcggtagagc
      421 gcacccgggt ttagcgcact acccggagtc agagtgagag acgtattacc accgccccat
      481 acaggggcat tcaccccaac acaattactg aagccatctg cagaaatctg caccgtgcca
      541 gtgcacgcgc cggcaacatt gttatacgac accgtgccga tgttaacagc gtgattgaat
      601 gccagcaaga atgtagtgga aggcgagaca ccagtcgaac catcggcggg tgtagtgagc
      661 gtaacggtga gagctggcgg ggcgtctgtc gtgaaaccga ccgaggtgaa atctccgctc
      721 agcgtttctg aaccggcacc ggtcagtacc gtcgttaccc gtacccgata gagcgtgctg
      781 taatttaacg ccacggctgg cgcaatgttg agctgcgtgt ccgcaaccat gaagacgtgt
      841 gaagggaaag cgatgcaatt gacaaagccg tcggcactca tctgaaaact gccggtgcac
      901 ggccccgcgg tcgagttgta gctcaatgta ccggtgctca ccggatgcgt gaagttgatc
      961 atcagcgccg tgtttacaga gacagcagtt gcgccttcgc tgggagacgc catggatggc
     1021 acgagtgtac ccggtggagc ggtcgtaaac cccgtcgtcg ttgtaaaggc gctgctgaga
     1081 acccgcccgc tcaccgccat aagcgttgtg agcactcgaa tcttatacgt agtgccattg
     1141 gcgagcgttg ccagaggcgt catggtgagt gaagtctggc tgctgcccca aaccggcgtc
     1201 gtgagtgcga tgcaggtgat aaaatcgtcg gcgcttacct gcagattgtt cgtgccgcag
     1261 gggccggcag ctgagttata cgtgagactg cctgtgttta cggtctcaga aaaattcagc
     1321 acaagctgtg tattatttgc gaccagcgca tcggtatcgg caggattcgt agtaactaca
     1381 gaaaaaacct gggttaactg cgtttctgct cccggcttta ttggtttcgg ccagttaaag
     1441 cccctgcagg acgcgataaa actcagaaca aaaaggcagg caatttttct ggcatgcatg
     1501 gaggatgatt cccgggagcg aaaagttata gccccttcgg aggatgcaga gtcgtgtgtt
     1561 taggttggcg gcaccggtaa agcgctaacc tgtcgtcgcc tgctacatac atttcgtaat
     1621 gatacggaaa aatccccgta tctgcgacat ccaaacggga taatatcaaa ttttcctccc
     1681 cctttttgca aaaaaggccg aaaaatgggt atggtacgct ctctctggac gtctgcgacg
     1741 ggcatgattg cccagcagtt tcacatggat acgatttcgc acaacctgtc gaacgtgaac
     1801 acaacgggat ataagaagaa tcgcgtcgat tttgaagacc tgctctatca acaccaggtg
     1861 ctcgccggta cgccttcgac tgcagtctct gagattcccg tcggggtgaa cgtcggtgtc
     1921 ggcgtgcgtc cagcagccac gcagaaactg ttccagatgg gttcgctgca gtcgaccggc
     1981 cacaagcttg acatggcgat ttcttccgac gtcggcttct ttaaaatact catgcccgat
     2041 ggcaccttcg gctaccagcg caacggggat ttcaaaatcg attcgcgccg gcaggttctg
     2101 acctctgatg gttataccct tgaaccgccg ctcgtgatac cacccgacgg cctcatcgaa
     2161 acactcacca tcaacgaaca gggcgaggtg cgcatccaga tcggcgacga ccagattccg
     2221 cgccagatcg gccagattga actctatcgt ttcgtgaacc cggccggcct taaagcgatc
     2281 ggcaaaaatc tgtacaaaga atctcccgcc agcggtcctg aaattccggg cacacctggc
     2341 ctgggtggct tcggtggtat cctgcagggt tttctcgaga tgtcgaacgt gaacctcgcc
     2401 gaagagatgg tcaacatgat cgtggcgcag cgtgcgtacg aagccaattc gaaatcgatc
     2461 cagacttcgg acagcatgct gtcgacagca atacaattga aacgtggtta ataactaagg
     2521 cggattgatt ggcgacatgg ataaataacc gttcaaaatt ctgtagagac gcgattaatc
     2581 gcgtctctac aagaggaatt gttatgggta ttttgtgcat gtcgttctca atggcccacg
     2641 cagaaaacaa gacccaatta gccctgaaat ccacatacgc tgcagccaaa tcttcgatct
     2701 gtctctctga cattattgag gcgcataacc ttgcgcagcg cgaagcggaa aaacttagcc
     2761 ggtactgccg catcgaattc aagggcgcgc gcatgacgct caacgccaaa gagatcgaac
     2821 tgcacgcctg ggctgcgggc gtcacgccgg ataaaatcag cggcggcccg gtgaccatca
     2881 gcatggcaac cacgctgcaa ctacctgcaa acaagaccgc tgcaccggtt aaactgcgcc
     2941 gtggttccgc ggtacagctt gtgctcaaga gcgagcacat gcagattgca cgcgaagcgg
     3001 cgctgctgca agatgctttt gccggtgaaa cagtcgacgt acgccccgcc ggcacgcgca
     3061 aaacgctgcg ggcaaaactg gtcagcaata ccgttgcgga gctggtgcaa tgagtggttt
     3121 cgtaaaagct gcattcgcat tggttgccgt ttcagcggca atcaatgcgc aggaatctct
     3181 ctggcgcgac cagaatccct atgtctcacc gccgcgcgac ggcgaaatcg tcgcgatcga
     3241 agtcaacgaa accttcaata tcgtcagcga cggccagtgg aaaagttcgc agcagttcga
     3301 gatgaaactg gtaccggata cgaaaaacat tccttttctc accagctcac agcagaacaa
     3361 gtcacgcagc aaaagttcaa acagcaacaa caaaacgcgc gacagctacc gctttcacct
     3421 gacgggaatc ctgggggcta aacaaccaga tggcaattac ccgatcaatg cacaaaaaag
     3481 cctgaacgtc gacggcaagc cggtcaatgt gcgcctcacc ggcatcatcg acccacgccg
     3541 tatccgccag ggtacaatcg aatcgcgttt cgtcggcaac ctttcgctga ctgtgcagtc
     3601 tgaaccgcct tttcccaaag accaaaagct cgacctgaaa ccgcccgcag gtactgaccc
     3661 gaattcgaaa ccgacgatga ctgaattcag cgatcagctg cgcaaagagc tgctgatcga
     3721 gcacatgaag cagattctcg gaggcatgaa tcaatgagaa gcgctatcgc gattctcatt
     3781 gctgccgcga gcgcagcgag cgtgcaggca ctgaatctca ccgtgcgcga catcacgcgc
     3841 atcagctggc accgcgagaa ccaggtttcg ggctacggcg tggtcatcgg cctcaacggc
     3901 agcggcgatt cgcgctctga gcttgcgctc gaaacactca agaaaaacct ctacaaccgg
     3961 gggattgacc tcggtgaaaa gactctgcag gcaaagaaca ttgcggccgt aatggtgacg
     4021 gcgatgattc cgacgcatgc tcgcccgggc gacccggtag atgtctgggt gtcttcgatc
     4081 ggcgatgcgc gcgccctgaa cggcggtatg ctcttgcaga ctgcgctcac cgctgccgac
     4141 ggccaggtat atgctgtggc acaaggcccc atcgcggccc ccctgccccg gccgacagag
     4201 aacaacaacc tgcaactcta caatttttcc gtctacaacc ggcgcatacc acccaacgac
     4261 cgcctcaacc aggaacgcca taactccgcc cacatacccg gcggaggtat tatagaacgc
     4321 ggtgtctcgc agccaaccgt actcgtcgat gacacaaaga agaccaagtc gtcgaaactg
     4381 tcactgcact attttgattt tgtgaccgcg cgcaacgtgg tggccgcgat caacaagaag
     4441 ctgcccgata ccgcatcgct tgccaatgac ggcaccatat ctctaaacat accctacgcg
     4501 agcgaccacg tcgactacct gagtaaaatc ctcgcggtca aagtcgacgt accggagcgc
     4561 accaaggtgg tgctcgacac gcgtaccggt acgatagtct ccggcggcag tgttgccatc
     4621 tctgccgtca tggtgacgca caacggcatg cagattgaag taagcggcaa gaaggcatat
     4681 gcatacggcg acgaaaaatc gccggccaac gccgagctca aagaaggcgc aacggtgaaa
     4741 gagctcgtcg acaacctgaa caagcttggt cttacctcgg ccgaaattat tgatgtcatc
     4801 aaggccatac acgcagctgg tgccttacac ggagaactga ctgtcctatg aacgtaaatg
     4861 ccattaaccc cctacttcta ccccagaaag aaacaccgcg cgtcggcaag accgacaaag
     4921 aatcggcagt gtcttttcag cagctgctga acgatcttgt gccgaaaaac ctcgatgcaa
     4981 aggtttcttc agaaaacttc agccgcacgc cgcagcgtga aaaaattctc gaacatgtcg
     5041 gtgcttcacc cgagcgcaaa aagatgtttg aagccgcgcg ccagttcgag aacttctttc
     5101 tcgaaaagat gttccgcgag atgcgcaaga acatgggcaa gaacgatatt ctgcatggcg
     5161 gttctgccga agatattttc caggacatgc tgctcactga acgtgtgaac tccctgacgc
     5221 agtcgtctac gcttggcctt gcagaacaaa tttatacgca gatgcagagg acagcctgac
     5281 aaagcaggtg ctgcgtgttc agaaccaaac ctaagccact ctgccccggc ccgacgaaat
     5341 tttttttcat agtactggct ttcagtagcc tgttcccgct gcatgcacag ggtgagacag
     5401 gcaacgtcgg gcgcaatgaa acacaggatg ccggcactgc tgaagctccg ccggcgtctt
     5461 ctgcccgacc tgccgcaggc agcgccacac aacccaaacg cccggtaaaa agcgcggctc
     5521 tcaagcgggc cgagaaactc tacttttcca ggaaatacga aacggctgcc gccttgttgc
     5581 gcaagatcac agacgaagaa ccggaaaacg gccgcgccaa ttctctgtta ggcgacgtct
     5641 actacctgca gggtcaatac gacaaggcgc taagccacct gctgcgtgcc gccgagctga
     5701 atgatcaaaa agcgccggac tacttccgcc tgggccagac gtacgaaaag ctgaacaaac
     5761 caaaagaggc tgtgcgggca taccagaaag cctatgaaag cgatgaggca atgaaagagg
     5821 ccttgttcca gattggtttt gtttacctga gtcttgagcg aaacaaaaag aagactatcg
     5881 aattctggca gcgttttgtc gacgaggctg taactgatcc gcaacgcgaa aaggttatta
     5941 ccgtcctgaa attcctgaat gacccgaatt ttgtactgcc cgacaagaat agcccgatat
     6001 cgctcgaaga agccctgatg ctcggcgggg gagctggcaa cggcgaagtg aaaaccggcg
     6061 aagacaaggc tgccggacac gagaaagaaa aagagaagaa cgacagtaaa gaacttctcg
     6121 acgacgacaa actaaactag aaacggggcc atgccgctct cgaacgatcg acgcagccag
     6181 caccgtgtaa ctgcgggcgc ttatatcgaa tacagggtca cgatagacgg gaccatggtt
     6241 aaagacgcca tcatgagcga tatttctgaa ggtggcctga tggtgcttgc tgaaagcgcg
     6301 gacatcggtc gtgagctcaa caaaaatgaa atgatttcgg gtgaaattat ccacgaccgg
     6361 acgcgcttcc acttcaagtt cgccggcgaa ctggcgtgga tccagccgcg cgaaatgcgc
     6421 acgcgtaaat ttgtcgcact gggcattcgt ttcaccccgg acgtacagct gccagcacag
     6481 atctacgcga ttcttatggc tgaccacgat ttctgattct ttacgttagc gccttatgaa
     6541 aacaatcata ctgcgcgccg gcaaggcgat tgccggatgg gtcaccgttc tgtgcctgat
     6601 ttttctgctt accgtgtcac tgcagcacct gcgtaaaggt tcggcagaag aagtactaat
     6661 ggggcaacgc gggaccaccc agtccctcgc tgcgctcaaa gataaatcat tcggctcacg
     6721 cctgaccgag ctggcagttg cactgcctgg aatggttcag tggaagacct tgcgcgggac
     6781 aatggtgttg ccacagattg ggcatgcgct cgcggcgacc gcaatgctga gcgcgctgag
     6841 cctgctgttt tcggtcgtga tcggcctcgt gatgttgtct gctgcagaat tttatccgcg
     6901 ttttgccaac cccctcgcca gcctggcgac agcaatcaac acgaccccga tattcctggt
     6961 gggaattttt ttcatctggt tattcgcttt tgcactgcgc ctcttaccct ctggggggga
     7021 cggcgggctg cgtttttttg ttctgccggc cctggcaatt gcagcaaaat tcggcgcgcg
     7081 tttgttcctg ctactcagga attacatgaa cgaacttgag aaccgggttt ttattctgcg
     7141 agcccgcgct tttggtttca acgaccggaa gattttcctg cacaagctgg caaactgtgc
     7201 aatgccgttc ataattttct ggctgatcga aacggcatcg ctcttttcag gtgcggtgat
     7261 cgtcgaatca ctgttctcca ttcacggtct cggccagctg ctgctcttcg cgctgctgca
     7321 atatgatatt cgcctcattt ttgcgaatct ggtggtcatc gcgaccatcg tctacctgac
     7381 ttcgctgctg cagaaggtta ttgcggaaac ccaggagcgc agtgcatgag cgacaggaag
     7441 atggcgtggc tcagtatcgc ggtactcgtg gcaattcttc ttgccggtat cctggcggcc
     7501 acaccaacaa tcaatctgga aaagaccttt cgcgctccgg gtagcgaatt ctggctgggt
     7561 tctaccccgc tggggcagtc ggtcgcacaa ctcgtactca ccggagcggt cagcacggta
     7621 ctactctctg caggggcgct cgttcttgct ctctctgctg cctttggctt cactggcctg
     7681 ttctatttgc tgccgcagcc agttcggaag gtttatgccg gtttcatcga tgcatggctc
     7741 gcgatcccgg gtatcttcat tgcactttcg atcggctatt tcctgccgca gagttttctt
     7801 tcggttatga ccgcgctcgt gctgtctgaa ttcgcaccgc tgcaaaagtt tctcctgcag
     7861 cgcctctcag gtatcggcca ggccgactac ataacgatgg cagctgtcat gggagctccc
     7921 gcagtgcacc ggctgcggtg gcacgtcatg ccaagactgg cacgggaagc cggcttcctg
     7981 ttcatcgtta cactaccctc tatcgcactt tcgctcgcga gcctcgagtt tctgggagtc
     8041 cagactggct cagagcgcat gtcactcggc ctgcagattg cgatctacaa agattatatc
     8101 tggctttacc cgcacctgag ccttgccccg gtcgtagttc tgctcatgac gctgtattgc
     8161 ctgggtagcg ctagccgtct gctgaaacag taaatacccc gaatcatacc cggtcgtccg
     8221 gtaatcctac catatccgag taccaatctg gagcaattct gatgcacaac ctaaccgatc
     8281 atcggtagtt ctacctatga aaacgcagga attacgcagc ttttttggcc ccacttaaga
     8341 taatgacacc attgtacatt aaaaggtaaa cagggattca ctgaatgtag cgcacgttta
     8401 gccctagccg accgaaaacc gcctatgaaa aacctagtca tcgctctaat tttccccttt
     8461 ttgataagat gtcggaactt tgattgggac gtgcacgaac ggctcgacgt ttagcgcccc
     8521 actggccatc gccactactt ctacattgaa atccgtcgcc tgcaaagccg gacaggcgaa
     8581 ctctcccgtt gcaagcgggt tgttctcgat tgacctgact ccgcccgctg cagccagctc
     8641 attcacagcc agtgccggcg acagtactgc gttgctttca tgggtgaatc ctggtgatgc
     8701 cgatttcagc cgggcgaagc tcgtgcgcaa ggcccccact cccccggcaa acagcgcaga
     8761 tggtacagta atctatgagg gtacgggttc tgccttcgcc gatctggggc tggcgaatgg
     8821 cacgacctat tattacgctg tgttcgctta tgacctcgcc ggtcacgagt cggcggcagc
     8881 aactgcaagc gcttcaccgc tggggggcct tttgaatgcg ccaacttatg tacccgccgc
     8941 cggaacctac aacctgccgc tgagtgctac gatcagttcg gccaatacgg gcgatatcat
     9001 ctgctatacc acgaacggta gcacaccggc ttgcgatgct acgcccacct gtacaacagg
     9061 cgcgctggct tcaggcagcg taacggtcgc ggcatcgcag acattgaagg caatcagctg
     9121 caagaccggc tcacccgttt ccgcggttgc accggccgct tacactattg atatgacagc
     9181 ccccgtattc agtggcctgt tgcccgccaa tggtgcatct gtcagctccg cgcaactcag
     9241 ttacagcctg tccgaaacct gcgtcaccgg ttcagtcacg tggacgcgca ctggcggctc
     9301 tgccgatgcg gggtcgcccc acacgagcgc gctcaccatg agcgaattgc ttgccggcag
     9361 ccaatcggcc attacgctcg tgaatactcc cccgcttgtg cagggagcaa tctacgacat
     9421 cggcttcagc tgcagcgacg ccgcgggaaa caactctccg ccggcgctga ttacagacgt
     9481 gatatatgat gcatcgccgc cggcagcgct atccgcattt cgcgcgatcg cgggcgatgg
     9541 gcgtattacc ctcgtgtgga acaacccggg gacctggatc tggcgggtgt gaagatcttg
     9601 cgcaagacgg gcggctggct ggacagcgac tcaggttcct cgggtatgcg aggtacacag
     9661 aactcagcga cggctgctgc ctggtcaaat ggtatcaact cctgcaatgc gccccgctcc
     9721 atttactgtg tggaacaata gccatttttg ttgacgccgc tcctgtaacc gtagtccctc
     9781 cgcctgtatg aaatcccctg tgcccgctgc ccgccctgtt acgcagcgca tttatgcaag
     9841 ccctaccctc gaccccatgt ataccccggt cgacgaggtt gccgttgtag agcgcgtagc
     9901 ccgctttaag acccgcagca taaaaaatga ggctaagctc gctggcatca agctcgccat
     9961 cagtatgatc gacctgacca ccctcgaagg caaagacagc cctggcaagg tcattcaact
    10021 ctgccagaaa gcgctgcgcc catcagaatt gcccggtgtg ccacaggttg ctgctgtctg
    10081 cgttttccca aacatgattc ctattgcaaa aaaggtgctc aaaggttcac aggtgaaaat
    10141 cgcctgtgtt tcgacggcgt ttcccgcggc gcaggtgccg ctcgatatca agattgaaga
    10201 cgtgcgccgc tcggttgagc ttggcgccga cgaaatcgac atggtcatct cgcgcggaca
    10261 gtttctcgcg ggcgaatata actatgtctt cgatgaaatc gcgcaggtta aagaagcttg
    10321 cggtaaggcg cggctcaagg tgattcttga gaccggcgag ctcgagacgc tcgacaatgt
    10381 gcgccgcgcg agttttatag ccatgcgcgc cggtgccgat tttatcaaaa cctcgaccgg
    10441 caaagtcacg gtcaatgcca ccatgccggt gacgcttgtg atgctcgagt gcatccgcga
    10501 ttattacgac gacaccggta ttcagattgg catgaaacct gccggcggta tccgcacatc
    10561 aaaagatgct ctccattacc tcgtcatggt taaagaaacc cttggcgacg cctggctcga
    10621 tccatattgg ttccgcttcg gcgcctcgtc ggtgctcaac gacctgttga tgcagtatgc
    10681 aaaacaggta aagggtggtt accagtcgat cgactatttc tcgctgagtt aagtgactca
    10741 ccgcctgaag ttattctggc agattgtaat cgccggtttg agtgcacggc tgagccggcg
    10801 tccgcaacgc ctggcaattc ttgccggcgc gggcgagctg ccgcgcatcg cggcagaaac
    10861 cgcgttcagc cgcaagatcg actttgtcgt ctatcacatt gttgaagc
//
LOCUS       DAOQJF010000073        10836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3117_length_10836_cov_5.095167, whole genome shotgun sequence.
ACCESSION   DAOQJF010000073 DAOQJF010000000
VERSION     DAOQJF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_10836_cov_5.095167"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLF85_05655"
     CDS             <1..990
                     /locus_tag="PLF85_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19180.1"
                     /translation="ISQAQIDAITTHYRNQAQANPRAATCGKPLKEGDLQRFLAGPYA
                     TPMVAVATDHAVAILVVSDALLPRLQKELELKIAGEPLYIAGVGSNFYDKYVSRRPDF
                     STPAKVAAERAFARSGVSPADIDYAWIYDCFTLMLVKQAARYFNLDFKAVADSLAKGY
                     IEFNGKKIHVNQSGGILNTQAAISLSAATGLIDILDHAQKNPQARTFLFGGNGGLDTI
                     NSVAILTRTPRPLQKARHTDAEHVLPQQPAQPLREDEVVTLYAAVMVRFNPGTDIPFA
                     LGAFRRADGSLCLLRIQDQAGKKLSTTDTLTRDKTQAQVRYVENKPLAVLLDN"
     gene            1171..2139
                     /locus_tag="PLF85_05660"
     CDS             1171..2139
                     /locus_tag="PLF85_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNE19181.1"
                     /translation="MDILNTLLQNLQTPMILAFFLGIAATLLKSDLKFPDGMYSGLTI
                     YLLLAIGLKGGVKLSSAPFAQIWQPALLAVLLCVLIPLLAYLVLRRFGGFDLTNAAAI
                     AAHYGSVSAVTFSEAQAFLDAQGVPYEGFMPSMLAIMEIPAILVALFLVKHKQKPGSG
                     MKKVVHELLTGKGTLLLVGGLAIGWISGKKGLDQFGPLFDAPFRGILMLFLLEVGIVT
                     GRRIGDLRQVGAFLVVFGVIFPVACAAATMPMAKLAGLSAGGAMIFATLAASASYIAA
                     PSAIRIALPEASPAYYLTASLVITFPFNIAVGLPLYLTAARILYGA"
     gene            2141..2443
                     /locus_tag="PLF85_05665"
     CDS             2141..2443
                     /locus_tag="PLF85_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19182.1"
                     /translation="MKLEKAKLVTIIADEAIENRVLDDLRALKVKGFTRSEATGEGLT
                     HAHISAWEGRNVRIETLVSPAKAHAILEVCANKYLDKYAMVIFTTDTEVYRQGRFS"
     gene            2564..3955
                     /locus_tag="PLF85_05670"
     CDS             2564..3955
                     /locus_tag="PLF85_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNE19183.1"
                     /translation="MQIPRLPAKPVYVPFGTQLDFFELFGRIEQAFETCFLLESMGEQ
                     SYDSRYSVIGFAPDFTFIGKPGEVEITSASGSERIKAENPYYYLREILPRDVLTRNYA
                     GGLVGYLGYDAANFFEPSLGVAFHGQYPAFQMGLFTDGLLHDKMTGDTTYFYFLHDRR
                     EQIAAAVAVPRRGESTSVQSLGQSKTREQHTTMVEAAKQEILDGNTFQVQIGFSESYS
                     ISGDWLNVYASLRQINPSPHMYFMKFHSGGKTFRLVGASPELLYRQRQGEMESFPLAG
                     TTRRGKTFEEDQQLVRTLLSDKKEIAEHNMLVDLHRNDLGRVARYGSVKVRRIFDVKK
                     FSHVQHISSEVVGLIAPGEDMFTALASSFPAGTLSGAPKIESMRIIDRIEQEARGPYG
                     GAVGHFGFNGDCTFAIPIRTIFGVDDSAFIRASGGVVYDSTAEGEYQEIQNKLAAVKK
                     ALSEYMPADGAKQ"
     gene            3955..4554
                     /locus_tag="PLF85_05675"
     CDS             3955..4554
                     /locus_tag="PLF85_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNE19184.1"
                     /translation="MRILLVDNYDSFTYNLYQYMGEVMEESGKPFRIDVVRNDEKSYE
                     DLNSASYDRVVISPGPGNPSDPKYFGVCTKILQNPAPTQKVLGVCLGMQGMAHVYGGK
                     VVRAALPMHGKTSPIEHDGKGVFAGLPQGIEVMRYHSLIAEEQSLPATLEITAKTTGP
                     GAKEIMGLRHKTLPVEGIQFHPESFATEGGKVMLRNFLL"
     gene            complement(4546..6546)
                     /gene="uvrB"
                     /locus_tag="PLF85_05680"
     CDS             complement(4546..6546)
                     /gene="uvrB"
                     /locus_tag="PLF85_05680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNE19185.1"
                     /translation="MKLKLHAPYKPAGDQPQAIAELAEALAPTDSKATLIGVTGSGKT
                     MTMAAVIEKLQRPTLIITHNKSLAAQLYREFSEFFPENAVEYFISYYDYYQPEAYVPV
                     TDTYIEKESSINDEIERLRLRATASLIEREDVIIVASVSCIYGLGSPETYKDSVLLLD
                     KSVKAGRDEVMAKLMRMQYARNDTELLQGRFRVRGDTIDVFPAYSNDPYRLEFFGNEI
                     EAINKFDLVTGKKTEALRRIAIYPAKHFITSPATLEHAIEDMKKELEAQLAYFKTVNK
                     PLEAERLRQRTTYDIEMLREMGYCSGIENYSRILTGRPPGSRPECLIDYFPENMLIII
                     DESHVTIPQIGGMFAGDRSRKQTLVDFGFRLPCALDNRPLNFEEFETLAKNVLYVSAT
                     PAAYELEHADAHVEQIIRPTGLLDPEIEIHGSEGQIDHLILEIDKRVKAGERVLVTTL
                     TKKMAEELTDYLVKASVKVRWLHSDIDALERLEILRDLRKGVFDVLVGINLLREGLDL
                     PEVSLVAILDADKEGFLRSHRSLIQTMGRAARNVNGKVILYADTMTDSMKSAIDETQR
                     RRKIQAEFNEKHGITPQTVKKEIKDFVERERVLEEDHWKRIEENLHEFSRKKFKSQDA
                     WKKAVEKAMFDAAKQLNFERAAELRDMLKQDDEELRSNSSSQ"
     gene            complement(6543..6875)
                     /locus_tag="PLF85_05685"
     CDS             complement(6543..6875)
                     /locus_tag="PLF85_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19186.1"
                     /translation="MQRTRDKHEDLKNLLTVSVMVFLLGGIVFAFLYRNIRMAIVTYE
                     IERLKKERRTAYIETEELRLQVARHSSADRMEKLFREKYGFLPVEVSQKISTYILPQI
                     GRKPQAPE"
     gene            complement(6883..7635)
                     /locus_tag="PLF85_05690"
     CDS             complement(6883..7635)
                     /locus_tag="PLF85_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19187.1"
                     /translation="MRTFARILVFFCLSSIAAAELVKGKRYDFYQDNGQNLLGAELLE
                     ESESSYIVKLSYLPKPLSIAKQNLLRPPQLSQGQAAETAPAFRWGRELRLNAQLGYTA
                     ITGGVLHDIFPSGYHGAIGADWHLFTAPRFGIRAVSVLGVFSQYETTPRSIRQLSLVA
                     GPQFFLFEWKQPQIIFTGSALAGAASVALKGYTFSSDYTVFAAMALVRAERSFGAVTV
                     GLQLMLNYLFDQSLNFTSSGLGLSVQYALNFK"
     gene            complement(7632..9116)
                     /locus_tag="PLF85_05695"
     CDS             complement(7632..9116)
                     /locus_tag="PLF85_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19188.1"
                     /translation="MHWVGRARDTGVVAALIWGGAFAVLAGSLLSTVTRLRMAELRDN
                     LQSANEKNDDLRNVALLSRVALINQRALSGGREDATQYRKEAESALITIKQTRGRSRQ
                     LGISDRLALPFLNGMNRILGLPRLKLGKMPEQELVLDLAFQFESFREYGKAVRGYTVY
                     LTDFSLGEDEKDFALLHRGFCHAMLNHFESAVADFAAVETSGSRRNADTARRLSQFLK
                     DLRERIRIIESVQDPARRGELYYEAAAYAKALENFALVEESRRSARLRFLTARSLEET
                     GQTSQALVIYRDLVRSAPDSPYALNANRRMYLLGTFLGHDEKLAEESRKNSETVVKDK
                     EFIAAVTHLEKSASQLHAEAAAEQAAEKSEIAAVEKVVLPETAVTPAKPEPVAQPPQA
                     PPQPEPVKPVEKPLAKPAVVDLRNAAAAQRAEALSVEKKEDLIRSQKEKIDKLTMIDG
                     NIFYGVAYRENQDSVWLFTVLGNLELKKADIRAREKVSGSAAIK"
     gene            complement(9116..10669)
                     /locus_tag="PLF85_05700"
     CDS             complement(9116..10669)
                     /locus_tag="PLF85_05700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19189.1"
                     /translation="MFRNLSLTARLSLLFFLFTMVNVVLFWLAVGSNQMRLLADKASL
                     QMHRTIVGIEQKLQIATATNPLSRRAEFYRSPAAEALLAQVFRDQDKATPDGLTEYSI
                     VSGSNTVHLSWPKELPRTELSADEVQNIVKTIRLREFSNEPFYTFPDVLNYRLTVYLP
                     FANDRGQYLILRAVFALDGMKTELSRLMRLGIAIVILLLVVQTALGFFLYRLIVRPLR
                     ELKIASQITGRGDFHQIAGYEKRRDEVGTLVAAFNKMSADIRDQKETIKKNFEEIRHR
                     DEMMQHELMIAQHIQKSIFPKSDYPHPVSLEYKPLYAVSGDFYDVYQFDDGSTGYLVC
                     DASGHGVPAALLTMMAKSAFATFARQHSEPGEVMQAANRHLADSLEMTGQYLTAFYVR
                     VSPGKIAYCNATHPEPVVVVSGQAEPVTLKSNGFYVGMLSDAPFEFQTETLATPKGSK
                     LVIVSDGITEARNATGELYGAQRLRSIVAASTALDVNALRDAIMTDLANFAAGTPAED
                     DVTLLVLEV"
BASE COUNT         2250 a         2934 c         3123 g         2529 t
ORIGIN      
        1 atcagtcagg cgcaaatcga tgccatcacg acgcattacc gcaaccaggc gcaggctaac
       61 ccacgggcag ccacctgcgg caaacctttg aaggagggtg acctgcagcg tttcctggct
      121 ggcccttatg cgacaccgat ggttgcggtt gcaacggacc acgccgttgc tatactggtt
      181 gtgagcgacg ccctgttgcc aaggttgcag aaggaacttg agctgaaaat tgccggcgaa
      241 ccactctaca tcgccggcgt cggctcaaat ttctacgaca agtatgtatc gcggcggcct
      301 gatttttcca cgcctgcgaa ggttgccgcg gaacgcgctt ttgcccgcag cggagtttca
      361 cctgcagaca tcgattacgc gtggatctat gactgtttta cgctcatgct cgtgaagcag
      421 gcggcgcgct atttcaacct ggacttcaaa gcagtggccg attctctcgc taaagggtac
      481 atcgagttta atggcaagaa aatccacgtc aaccagtcgg gaggaattct caatacccag
      541 gctgcgattt cgctttctgc tgcaacgggc ctcatcgaca tcctggatca tgcgcagaag
      601 aatccacagg cgcgtacctt tctcttcggt ggaaacgggg gcctcgatac gataaactcg
      661 gtagcgattc tgacccgcac gccgcgtccc ctgcagaaag cacgccatac cgatgctgaa
      721 cacgttttgc cgcaacagcc tgcgcagcca ctgcgggaag atgaagtggt tacgctgtac
      781 gcggctgtca tggtgcgctt caatcctggc acggacatac catttgccct gggggcattt
      841 cgccgcgcgg acggttcgct ttgcctgctg cgaatccagg accaggctgg caaaaaactc
      901 agcacaacag acaccttaac ccgggacaaa acacaggctc aggttcggta cgtagagaac
      961 aagccgctcg cagtgctctt ggacaattaa gaagctggcg ccgccacggc gagggtagaa
     1021 ggcccggtta atacgcgact tttttcgtca aagcgcaaat cagtaagtat aatacatgaa
     1081 atgagattca tgtatgagtt tgtgcaaaag cggggtgtgt gctgatgaga aaacccggaa
     1141 aatgtatggg gcaggcccgg actcaatagc atggatatcc tgaatacgct gctgcagaac
     1201 ctgcagacac cgatgatttt agccttcttt ctcgggatcg cggcaacatt gctgaagagc
     1261 gacctgaaat ttcccgacgg catgtattca ggtctgacga tctacctgtt gctcgcaatc
     1321 gggctcaaag ggggagttaa gctgagctca gcaccttttg cccagatctg gcaaccggct
     1381 ttgctggcag tcttgctgtg tgtacttata cctctcctgg cttacctggt cttgcggcgg
     1441 tttggcggtt ttgatctcac taacgctgcg gcgattgccg cccactacgg ttcagtgtcc
     1501 gccgtcacat tcagcgaggc gcaggcattc ctggatgccc agggtgtgcc gtacgaaggg
     1561 ttcatgccca gtatgctcgc tatcatggag attccggcga ttctcgtcgc gctgtttctc
     1621 gtgaagcata agcagaagcc gggttcgggg atgaagaaag tggtgcacga attacttacc
     1681 gggaaaggta cgctgctgct ggttggtggg ctggcgattg gctggatttc aggaaaaaag
     1741 ggattggacc agtttggtcc actcttcgat gcaccgtttc gcgggatcct catgctcttt
     1801 ttactcgagg tcggtatcgt gaccggcagg cgaattggtg atctgcgcca ggtcggtgcc
     1861 ttcctcgtcg tttttggggt aattttcccg gtcgcctgcg cggcagctac aatgcctatg
     1921 gcaaaacttg ctggcctgtc ggccggtggg gcgatgatat tcgcgaccct ggcagcgagt
     1981 gcctcgtata tcgccgctcc ttctgccata cgcatcgccc tgcctgaagc aagtcccgcg
     2041 tactacctga cagcttcgct cgtcatcacc tttccgttta atatcgctgt tggtttgccg
     2101 ctatacctca ccgcagcacg aatactctac ggagcctgaa atgaaactgg aaaaagccaa
     2161 gctcgtgact ataattgccg acgaggcaat tgagaaccgt gtgctcgatg atttgcgtgc
     2221 actcaaagtg aagggtttca cgcgaagtga agcaacaggc gaagggctca cccatgccca
     2281 tatatctgcc tgggaaggca ggaacgtgcg catagaaacg ctcgtttcac cggcgaaggc
     2341 tcacgctatc ctggaagtat gcgcgaacaa atacctcgac aagtatgcga tggtgatatt
     2401 cacgaccgat actgaggtgt accgccaggg ccggttcagc tgattcacgc cgggccatca
     2461 tagggtatag cgatggccct atcgggtcct ggcaccctcc gtctccaccc tgttgcgcgg
     2521 aattcagatt ttccgcgtga ctgacgcccc aagactgcct cgaatgcaga ttccgcggct
     2581 gccggcaaaa cctgtctatg ttccgttcgg gacccagctc gattttttcg agctctttgg
     2641 ccgtattgaa caggccttcg aaacctgctt tctgcttgag tctatgggcg agcagagtta
     2701 tgactcccgc tattcggtga tcggttttgc gcccgatttc actttcatcg gtaaaccggg
     2761 cgaggttgaa attacttcgg cttccggctc tgagcggata aaagcggaga acccgtacta
     2821 ctacctgcgc gagattctgc cgcgcgatgt gttgacgcgc aactatgccg gcggccttgt
     2881 cggttacctc ggctacgatg cggcgaattt tttcgagccc tcgctcggcg ttgcgtttca
     2941 cgggcaatac ccggcgttcc agatggggct tttcaccgat ggcctgctgc atgacaaaat
     3001 gaccggcgac accacgtatt tttatttttt gcatgatcgc cgtgaacaga ttgcagccgc
     3061 cgttgccgtt ccgcgcaggg gcgaatcgac aagcgtgcaa tcgctgggcc agtcaaagac
     3121 gcgcgaacag catacaacca tggttgaagc cgcgaaacag gaaattctcg acggcaacac
     3181 tttccaggtg cagataggtt tcagcgaatc gtattcgatc agcggtgact ggctcaatgt
     3241 ctacgcaagc ctgcggcaga tcaacccgtc gccgcacatg tacttcatga agtttcacag
     3301 tggcggcaaa acttttcgcc tcgtgggagc gagtcctgaa ctgctttacc ggcagcggca
     3361 gggcgagatg gaaagttttc cgctggcggg cacgacgcgg cgcggcaaaa cctttgaaga
     3421 ggatcagcaa ctggtgcgca cactgctctc tgacaagaaa gaaatcgccg aacacaacat
     3481 gctggtcgat ttgcaccgca acgatctggg cagggttgca cgctacggtt cggtaaaggt
     3541 caggcggatt tttgacgtga agaaattttc tcacgtgcag catatctcga gcgaagtcgt
     3601 ggggctgatt gcccccggcg aagatatgtt tacagcgctt gcttcatctt ttcccgccgg
     3661 cacattgtcg ggtgcaccga agattgaatc gatgcgcatc atcgaccgca tcgaacagga
     3721 agcgcgtggg ccttatggcg gcgctgtcgg gcattttggt tttaacggtg actgcacttt
     3781 cgcgattccg attcgaacaa tctttggcgt cgatgacagc gcattcattc gcgcctcggg
     3841 tggggtggtg tacgattcaa cagccgaagg cgagtaccag gaaatccaga acaagctggc
     3901 ggcagtcaag aaggcgctgt ctgagtatat gccggctgac ggagcgaaac aatgatccgc
     3961 atcctgctcg tcgacaacta tgactcgttc acctacaacc tgtaccagta catgggcgag
     4021 gtgatggaag aatcaggaaa accgtttcgc atcgatgtcg tgcgcaacga cgaaaagtct
     4081 tatgaagacc tgaactccgc ctcgtacgac cgcgtggtga tttcgcccgg gccgggtaac
     4141 ccctcagacc ccaaatactt tggcgtctgc acaaagattc tgcagaaccc ggctccgaca
     4201 cagaaagtac tcggtgtctg cctcggcatg cagggtatgg cgcacgtcta tgggggcaag
     4261 gtggtgcgcg cggcgctgcc gatgcacggc aaaacctcgc cgattgaaca cgacggtaag
     4321 ggtgtgtttg ccggtctgcc gcagggcatt gaggtcatgc gctaccacag cctgatcgcc
     4381 gaagagcagt cattgccagc aacacttgag atcacggcca aaaccaccgg gccgggcgca
     4441 aaagagatca tggggcttcg ccacaagaca ctgccggtcg aaggtataca gtttcatccc
     4501 gagtcgtttg cgacggaggg cggcaaagtc atgctcagga atttcttatt gtgatgaaga
     4561 attgcttcgc aattcttcat catcctgttt cagcatatcc cgcagttctg ccgcacgctc
     4621 gaagttgagc tgcttggccg catcgaacat cgccttctcg acagcttttt tccaggcgtc
     4681 ctgcgacttg aactttttgc gcgagaattc gtgcaggttc tcttcgatgc gcttccagtg
     4741 gtcttcttcg agtacgcgtt cgcgttcgac aaaatctttg atttcttttt tgaccgtctg
     4801 cggcgtgatg ccgtgtttct cgttgaactc cgcctgtatc ttgcggcgcc gctgcgtctc
     4861 gtcgattgcg ctcttcatcg agtcggtcat ggtgtcggcg tagagtatta ctttcccgtt
     4921 cacgttacgt gccgcgcggc ccattgtctg tatcagcgag cggtgcgaac gcagaaaacc
     4981 ttctttatcg gcatcgagta tcgccacgag cgatacctct ggcaaatcga ggccttcgcg
     5041 cagcaggttg atgcccacca gaacatcaaa cactcccttg cgcaggtcgc gcagaatctc
     5101 gaggcgttcg agtgcgtcga tgtcagagtg cagccagcgc accttgacgg aagctttgac
     5161 caggtagtcg gtgagttctt cggccatctt cttggtaagc gtggtgacga gcacacgctc
     5221 gcccgctttc actcgcttgt cgatttccag tatcaggtgg tcgatctggc cttcgctgcc
     5281 gtgaatttca atttcagggt cgagcagacc cgttgggcgg attatctgtt cgacgtgcgc
     5341 gtcggcgtgc tccagctcgt aagcggcggg tgtcgccgag acatagagga cattttttgc
     5401 gagggtttcg aactcctcaa aattgagcgg gcggttgtcg agcgcgcagg gcaggcgaaa
     5461 gccgaagtcg acgagcgtct gcttgcgcga ccggtcgccc gcgaacatgc cgcctatctg
     5521 tggaatcgtg acgtggcttt cgtcgatgat gatcagcata ttctcgggaa agtagtcgat
     5581 gagacactcg gggcgcgagc cgggcggcct gccggtcagg atgcgcgagt agttctcgat
     5641 gccgctgcag taacccattt cgcgcagcat ctcgatgtcg taggtcgtac gctgcctgag
     5701 gcgctcggct tcgagcggtt tgttgacggt cttgaagtag gcgagctgag cttcgagctc
     5761 tttcttcatg tcttcaatgg catgctcgag tgtcgcgggc gaggtgataa agtgtttggc
     5821 ggggtagatc gcgatgcggc gcagcgcttc ggtcttcttg ccggtcacca ggtcaaattt
     5881 gttgattgct tcaatttcgt ttccgaagaa ttcaagccgg tacgggtcat tggaatatgc
     5941 aggaaaaaca tcgatggtgt cgccccgcac acgaaagcgt ccctgcagga gctcggtatc
     6001 attgcgcgcg tactgcatgc gcatgagttt tgccatcacc tcgtcgcggc cggctttgac
     6061 cgacttgtcg agcagcagca ccgagtcttt gtaggtctcg ggagaaccga ggccatagat
     6121 gcagctcacc gatgcgacga tgatcacatc ttcacgttca atcagcgacg ctgtagcccg
     6181 caggcgcagg cgttcgatct catcgttaat ggacgattct ttttcgatgt aggtatccgt
     6241 tacaggcaca taagcttcag gctggtaata atcgtagtag ctgatgaagt attccaccgc
     6301 gttttcagga aaaaactcgc tgaactcgcg gtagagctgt gcggccagcg atttgttgtg
     6361 cgtgataatg agcgtggggc gctgcagctt ttctatcacc gccgccatgg tcatggtctt
     6421 gcccgaaccc gtgaccccaa tcagtgtggc cttgctgtct gtcggtgcga gggcttcggc
     6481 aagttctgca attgcctgcg gctggtctcc ggccggtttg tagggcgcgt ggagtttcag
     6541 tttcattcgg gggcctgtgg cttgcggcca atttgaggta gaatataagt actgatcttc
     6601 tggctgactt ccacaggtaa gaaaccgtat ttttcgcgga acagtttttc catacggtcg
     6661 gctgacgaat ggcgcgccac ttgcaggcgg agctcttctg tttcgatata cgcagtcctg
     6721 cgttcttttt tcagccgctc tatttcgtac gtaacaattg ccatgcgaat attgcggtaa
     6781 agaaatgcga agacgatacc acccagcagg aataccatga ctgacacggt cagcaggttc
     6841 ttgagatctt cgtgtttgtc gcgtgtccgc tgcattgacg gtctatttga aattgagggc
     6901 gtactgcaca ctcagtccta acccggacga agtaaaattc agcgattgat cgaacagata
     6961 atttagcatc aactgcaggc cgacagttac ggccccaaag gatctctcag ccctcaccag
     7021 ggccatggcg gcaaatactg tgtaatcaga cgaaaaagta taacccttga gcgcaacaga
     7081 ggcggcacct gccagtgccg agcccgtgaa gataatctgt ggctgtttcc attcaaataa
     7141 gaagaactgc ggcccggcca ccagggacaa ttggcggata gagcgcggag tggtctcata
     7201 ttgcgaaaac acacccagca cgctgacggc tcgtatgcca aaacgcggag ctgtaaacag
     7261 gtgccagtca gctccgatcg ccccatggta acctgacgga aaaatatcgt gcagcacacc
     7321 gccggttatc gcggtataac ccagttgtgc attcagtcgc agttcacgcc cccagcgaaa
     7381 agcaggcgcg gtctctgcag cctgcccctg ggataattgc ggcggtcgca acaggttttg
     7441 ttttgcaatg ctcagcggct ttggcaggta gctgagttta actatgtagg agctttctga
     7501 ttcttccagc agttctgccc ccagcaggtt ctgtccgtta tcctgataaa aatcgtaccg
     7561 tttgccctta acgagttcag ccgcggcgat cgatgacagg cagaagaaaa ccagtattcg
     7621 cgcgaaagtc ctcatttgat ggccgcgctg ccagatactt tctcacgcgc gcggatgtcg
     7681 gctttcttga gttccaggtt accaagcacg gtgaatagcc aaaccgagtc ctggttctcg
     7741 cggtaggcta cgccgtaaaa aatattacca tcgatcatcg tgagcttgtc gatcttttcc
     7801 ttctggctgc gtataagatc ttctttcttc tcgacagaaa gcgcttccgc tcgctgtgcc
     7861 gcagcggcat tgcgcaggtc gacgactgcg ggttttgcca ggggcttctc tactggcttg
     7921 acgggttccg gttggggggg ggcctgtggc ggttgagcga caggttcggg tttagcggga
     7981 gtgacagccg tttcaggcaa cacaaccttc tcgacggcgg cgatctccga tttttctgct
     8041 gcctgttcag cggccgcctc agcgtgcagc tgcgaggccg atttttcaag gtgtgtgacg
     8101 gcggcgatga actctttatc tttgaccaca gtctctgaat tcttgcgcga ttcttcggcg
     8161 agcttttcgt catggccaag aaaggttccc agcaggtaca tgcggcggtt cgcgttgagt
     8221 gcgtatggcg aatcgggtgc ggagcgtaca aggtcgcggt agattaccag tgcctgcgag
     8281 gtctgcccgg tttcttcaag gctgcgcgcc gtgagaaagc gtagccgcgc cgagcgccgc
     8341 gattcttcca ccagcgcaaa attctcgagt gccttcgcat aggcggcagc ttcgtagtac
     8401 agttcgccgc gccgtgccgg atcctgcact gattcgatga tacgtatgcg ctccctcagg
     8461 tctttgagaa actggctcag gcgccgcgca gtatcggcgt tgcggcgcga cccgctggtt
     8521 tcgaccgcgg caaaatcggc aaccgccgac tcaaagtggt tcagcatcgc gtggcagaaa
     8581 ccacggtgca gcagcgcgaa atctttttcg tcttcgccca gggaaaaatc tgtcaggtag
     8641 actgtatatc cccgcaccgc cttgccgtat tcgcggaacg attcaaactg gaaggcgaga
     8701 tcgagtacca gttcctgctc gggcattttg ccgagtttca ggcggggtag accgagtatt
     8761 cggttcatgc cgttgagaaa cggcagagcc aaacggtcag aaatacccag ctgcctgctg
     8821 cggccgcgcg tctgtttgat cgtgatgagc gcgctttctg cttctttgcg gtattgcgtg
     8881 gcatcttcgc gaccgcccga aagggcacgc tggttgatca gggccacgcg cgaaagcagc
     8941 gcaacgttgc gcaggtcgtc attcttttcg ttggccgact gcaggttgtc gcgcaactct
     9001 gccatgcgca ggcgggtgac ggtgctgagc aggctgccag ccagtacggc gaatgcgccg
     9061 ccccagatga gcgctgcgac aacccccgta tcacgcgcgc gccctaccca gtgcatcaga
     9121 cctcgagtac caacagggtg acgtcgtctt cggcgggggt gccagccgcg aagtttgcca
     9181 ggtcggtcat gatggcatcg cgcagcgcat ttacatcgag cgctgtcgaa gcggcgacaa
     9241 tgcttctgag ccgctgtgca ccataaagtt caccagtggc gttgcgcgct tcggtgatac
     9301 catcgctgac gatcactagc ttactgccct taggagttgc gagcgtttcg gtctggaatt
     9361 caaacggtgc gtcggagagc ataccgacat agaagccatt cgacttgagg gttacaggct
     9421 cagcctggcc ggaaaccacc accacgggtt cgggatgcgt tgcgttgcag tacgcgattt
     9481 tgcccgggct gacgcgcaca tagaaggcag tcaggtattg cccggtcatt tccagcgaat
     9541 cggcgaggtg gcgatttgcc gcctgcatga cttcgccggg ttcactgtgc tgcctcgcaa
     9601 aagtagcaaa tgctgatttc gccatcatcg tcagcaaggc ggcgggaacg ccgtgacccg
     9661 aagcatcgca gacgaggtat cccgtgctgc cgtcgtcgaa ctggtaaaca tcgtaaaagt
     9721 ctcccgaaac ggcgtagagt ggtttgtatt cgagcgacac ggggtgcggg taatcacttt
     9781 tcgggaaaat acttttctgg atgtgctgcg cgatcatgag ctcgtgctgc atcatttcgt
     9841 cgcggtgcct gatctcctcg aaattctttt tgatggtttc tttctggtcg cggatatcgg
     9901 cagacatctt attgaaggct gcgaccagcg tgccgacttc gtcgcggcgt ttttcgtaac
     9961 ctgcaatctg gtgaaaatcg ccgcgaccgg taatctgcga agcaatcttc agctcgcgca
    10021 atggacgcac tatcaggcga tagagaaaga agccgagggc agtctgcaca accagcagca
    10081 ggataacaat tgcaataccc agtcgcatca gccgcgaaag ttcagtcttc atgccatcga
    10141 gagcaaacac ggcgcgcaaa atcaggtact gtccccggtc gttggcaaat ggcaggtaaa
    10201 ccgtgagccg gtagttcagg acgtcgggaa aagtatagaa cggttcattg ctgaattcgc
    10261 gcagccgaat cgttttgacg atattctgta cttcgtcggc agatagctcg gtgcgtggga
    10321 gctccttcgg ccatgaaagg tgcaccgtgt tactgcctga cacgatgctg tattcggtta
    10381 aaccgtcggg tgttgccttg tcctggtcgc gaaagacctg tgcgagcagg gcttcggcag
    10441 ccggcgagcg gtagaactcg gcacgccggc tcagcggatt ggtggctgtc gcgatctgca
    10501 gcttttgttc gatgccgaca atcgtgcggt gcatctgcag cgaggccttg tcggcaagca
    10561 ggcgcatctg gttcgagccg acggcgagcc agaagagtac cacgttcacc atggtgaaca
    10621 ggaagaacaa cagactcagt cgcgccgtaa gtgaaagatt gcggaacata tagccttaac
    10681 ggtattgcag ggggggtagt gttcgatagc ctgtaaagac tttgcctcgg cttcgtcttg
    10741 ccgccgtgtg gcagctaccg tgtccgtgac tgtgcgtttc ttcgcccatc tgctgctcct
    10801 tttggcgctg tgcgccggcg gctgcgcgac ccttga
//
LOCUS       DAOQJF010000074        10835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3118_length_10835_cov_4.784694, whole genome shotgun sequence.
ACCESSION   DAOQJF010000074 DAOQJF010000000
VERSION     DAOQJF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3118_length_10835_cov_4.784694"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PLF85_05705"
     CDS             complement(<1..1144)
                     /locus_tag="PLF85_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE19190.1"
                     /translation="MAKKGGPARKSSPASSRSGSGKKAASPRFNTGRDSKDAATGPII
                     RSLPGSLGNERTTETTKRPLWKKILVAVALIAVLAFTWVTCKARLSVADETGTIKVSG
                     ISGAVTIARDELGVPFVKADSEQDAFFGAGYAAASDRLWQMYSTKLAVTGRLAEIAGS
                     KMLPVDVYMRSVGVKRNVDRAIDKMPAENRAPLEAYAAGINAYLQTHKHLPIEFILTG
                     YTPEPWTIYDCGYVFGALSFGLALNINEELFFIEAAKQMGAEKAAWLVPTYQDEPIPF
                     AEAQKIDKKIASSLTPETQMLRDTLENLNQIFRTGMPASNNWAIAGSKTKGGKAIVAN
                     DTHLPITMPALWYMMSIESPTYKAAGVTIAGQPFIQLGYNGNIAWGA"
     gene            1287..2447
                     /locus_tag="PLF85_05710"
     CDS             1287..2447
                     /locus_tag="PLF85_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNE19191.1"
                     /translation="MAVEAAVSTQYVPVTGAEKSSRNQSKGAIQYLRPTLSRNELKSV
                     LESLVMEEVSFGQGVLNFEKAFARTFDFQHAAAVDSYTSAFHLALLACELNKEDEIIV
                     PVSAPLAFFDAVNYVGAKVVLVDLAKDSFHCDIDQLFAAVTEKTKAVFLPYSFGSYKD
                     FRPFYEKLEAAGLRKGKEQRVRVIEDISHVVGHDYNGRQAGSDADIAIAGLNEDHLMT
                     IGKGGVVMTDSNNLFALIKDLRVQGGNRPYRVRFDYAITDYQAAMGMEQLGNLAAIIE
                     RRRRMGVLYNEAIASSRLKTYFVAPEADVCGAFPVIGEAEIGHMQRYFSSLQIETRRV
                     FPNGPLHHIMGLNPLEYPNAEKIYQRGLLLPLYPMLNKAAVEKITAAIRGFY"
     gene            2686..2880
                     /locus_tag="PLF85_05715"
     CDS             2686..2880
                     /locus_tag="PLF85_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19192.1"
                     /translation="MSDKQQKFYQCPECTSIVERVYQNGVCKSCLSKKFQAVRLVIDH
                     NHALQDAQAKVGRVSRALSR"
     gene            2877..3902
                     /gene="rfaE2"
                     /locus_tag="PLF85_05720"
     CDS             2877..3902
                     /gene="rfaE2"
                     /locus_tag="PLF85_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNE19193.1"
                     /translation="MNAASSYSVILDRDKTINQDPGYLADPAAVVLMPGAAEGIALLN
                     HFGIRVFVASNQSGVSRGLISPQQLKAVNARIEELLREGGAHIGGFFICPHQDHEECH
                     CRKPKPGLVKQIIETHRIDVERTFIVGDRARDLECGQSFGIRGILVGDEETQRPQNLV
                     FQANNLHEAAAYILETIFEEQTQKKFFASVHAFMPELHKQRAAGRRIVFTNGCFDLLH
                     SGHVQLLSQARALGDYLVLGLNSDRSVSALKGPTRPVNSETDRGRILAQLPYIDAVVV
                     FDEDTPVEMMKIIQPDIQVKGGDYVKEKLPEFPVMQAMGKEIVILPFRKGYSTTGILA
                     KSNQRAP"
     gene            complement(3899..5185)
                     /gene="xseA"
                     /locus_tag="PLF85_05725"
     CDS             complement(3899..5185)
                     /gene="xseA"
                     /locus_tag="PLF85_05725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNE19194.1"
                     /translation="MPQQYTVAELCRVIEYKIKNSPELQDVWVKGEITSYQKHASSGH
                     IYMTLSDTSTKGANPRPTIRCTYFRFAQTALDFELKAGIEVEVLGSTGIYAPQSTYSL
                     TVKKVQKIGAGDLLQRLQLIREKLLKEGLIRTDNRRALPLLPKRVGIVTGAGTAAYRD
                     MLKQVQERYPNAEIVLAPAQVQGEAAPQSIVAALKEIQKPEWRCEVIIVGRGGGSAED
                     LMAFNDEAVCRAIATCRVPVVSAVGHQIDHPISDDVADYAAATPTDAARQVFPVVDEL
                     KNQSEQLLRRAYAAVEAELRVMQERFLRTAEKEFWEKPYVLVEEQSRWLDELESKLHF
                     TFRNMVHTAANSLNQLTPLGVLVQQKLRLTRAEFESTSGRFEAYSPLATLRRGYSVVY
                     AGDKIVTDAAALKHGETIRVRLAKGEVKANVESTGS"
     gene            5239..7575
                     /locus_tag="PLF85_05730"
     CDS             5239..7575
                     /locus_tag="PLF85_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNE19195.1"
                     /translation="MQSYLITQCLQNDFVRLLDRYDPLPNYLHIGYDEANRLLGERVE
                     EGPVAQIIRWAYNETPDRLQIVHIRDWHDAADAEQQAHLAQFGAHCLKGSEGAEFVFN
                     RQVVPGRKAAIVDASGLNDFYKTDLAGYLSPCLEAPTRIGLIGVWTEAKITYLAYELT
                     TRYPKAEIITCSALTASSSRSQHFIALDHLRKILGVKVFDSVASFAEYLGGSGHGLTA
                     SAYAQNKQLDDTKFRFNIPYELKEADNKILHYLFRDSKSADFICLDGGFSGNVVLKAE
                     SRDAYGQKQQPTVVKIGQRDPISRERASFERIQEVLGNSAPAVVDFAEFGERGGIKYR
                     YAAMTDGKVRTFQKYFAETDDVELLKKFLQVVFRRQLGRFYEAASQENLNLLEYYDFR
                     SKYADSVRKKCAELSELRPDGRLQIAPGIDVEDVSRFYAEDLQHLRETVAVPRYVSWL
                     HGDLNGANIIIDESQNVWLIDFFHTHRGHALKDLIKLENDILYIFTKIENAGDLRLAC
                     LATDWLYAVHDLAVPPQDMPAELAADKRFAKSLAVICYLRSLYRGIIDLDRDPYQFFV
                     AAARYAMHTLSFDECNLWQRKWALYSGAKALAAVRDALTKTARLRIDFMTVAGEPASG
                     EPSATGLLGMTILPGRKDRGRDLSADLDEMKKQKVAHVATLISNNEFGEYGVGNLLEA
                     YKAAGFDFIHRAVKDQGVPTRQQAEEVLVWIKERTDRGENVAIHCVGGLGRTGIVAAL
                     YLKKYAGLKAEQAIERVRQVRSPRAVENQEQENFIKDF"
     gene            7617..9017
                     /gene="sthA"
                     /locus_tag="PLF85_05735"
     CDS             7617..9017
                     /gene="sthA"
                     /locus_tag="PLF85_05735"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNE19196.1"
                     /translation="MPESTIARYDVVVIGSGPAGEGAAMQCAKDGKSVAIIELNAQLG
                     GECLHRGTIPSKALRHSIQRYIEAMSNPLVKGHFSGRDLEFPQVMAAARRIIDREADH
                     NLGYYERNRVKIYGGRARITAPHRIEVDLGRAGTETIEADNIIIGTGSRPYRPANVPF
                     DDRILDSDTVLDLAHTPRSLTIYGAGVIGCEYASIFKGLGIKVTLINHRAKLLEFLDD
                     EIIDALSYHLREQGVVIKHGEEYEKVERAGDEVILTLKSGKIIKSEALLWANGRSGNT
                     NGLFGFESGIEIDHRGQVVKNENFQTALPHIYAVGDVSGPPALASAAFSQGRFAALHI
                     TRGMAIGGLVRGIPTGIYTNPEISSLGQTERELTAQKIPYEVGQAHFRNIARAQITGQ
                     TAGLLKILFHRESLEILGIHCFGDQASEIIHIGQAIMSQPSPHNRITWFVETTFNYPT
                     MAEAYRVAALNGLNRL"
     gene            complement(9020..>10835)
                     /locus_tag="PLF85_05740"
     CDS             complement(9020..>10835)
                     /locus_tag="PLF85_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE19197.1"
                     /translation="QTPQGPLTYRELAQQVDRLAAFLQQRLHLKKGAAVIVLADNHPL
                     WLLVALAVQAVGSVEFPLSPLAGSREIASLVKSTRCRHFFTADAAGTRHIRALLASAH
                     CIQLSGVPPAHKNITALETILRETQAVAFHHEGSAVSAVIRTSGTTGTPKQVPVLHAS
                     FLHSMRVIPRVLRMGPRDVVLSCLPSWHLYARLVEYTALACGAQIVYAPIHGLQQALR
                     TSGCTIFPSFPEIWEKIHHEILVQLGQGFVGKMLRRLIRLSVHTNRIFESTASRSRYA
                     KSNRSIFAWLQLAYLLPVRTALMATLFAAIRRRVSPTLRYAIMGDAPLPLAVDETLRA
                     LGFEVLEGYGSTEQCVTALRRPGRHFPGVVGRVLPGVEVAIYSTLACEWNGMTLGEIA
                     VSGDNVFPGYFSSFTALGSVTAATPTYLTGDIGYVDNARNLIVVGRKANVLQLANGEL
                     VFPELVENVLRGNRFVGRVVVLGGGTNPLFALVVPDFVALLAWLEPHGGHDAKLADEP
                     ESDKKFWQSVTQSVAVRSLYAREFTRLLGDSGLPAYAWPVGFSLVPHLFHRGRELTPT
                     LKIRRAQVARLYQKEMRLWRHPDKMDANLRLIHTNRER"
BASE COUNT         2337 a         3064 c         3135 g         2299 t
ORIGIN      
        1 tggcacccca ggcaatgttg ccattataac cgagctgtat aaacggctgc ccggcaatag
       61 tgacgcctgc agccttgtag gtgggtgatt caatgctcat catgtaccag agcgccggca
      121 tcgtgatggg caggtgcgtg tcgttggcga caatcgcctt gccgcccttg gtcttgctgc
      181 cggcaatcgc ccagttgttt gaggcaggca tgccggtgcg gaagatctgg ttgaggtttt
      241 cgagcgtgtc gcgcagcatc tgggtttcag gcgtgagaga cgaggcaatt ttcttgtcga
      301 tcttctgtgc ttcggcaaaa ggaatcggct catcctggta ggtcggaacc agccacgcag
      361 ccttttccgc tcccatttgt ttagccgctt ctataaagaa gagttcttcg ttgatattga
      421 gcgcgagacc gaacgaaagg gcaccgaaca cataaccaca gtcgtagata gtccagggtt
      481 cgggagtata accggtcagg ataaactcga tgggcaggtg cttgtgggtc tgcaggtatg
      541 cattgattcc cgcagcatac gcttcgagcg gtgcgcggtt ctcggcgggc atcttgtcaa
      601 tcgcgcggtc gacgttgcgt ttcacaccga cgctgcgcat gtagacgtcg acgggcagca
      661 tctttgaacc ggcaatttcg gcgagacgac cggtgacggc gagcttcgtc gaatacatct
      721 gccacaggcg gtcggaagcc gctgcgtaac ctgcgccaaa gaatgcatcc tgttcagaat
      781 cggctttaac gaaggggact cccagttcat cgcgcgcaat ggtcactgcg ccgctgatgc
      841 ctgagacttt tatggtgcca gtctcatcag cgactgaaag gcgtgccttg caggtgaccc
      901 aggtaaacgc gagcacagcg atcagtgcca ccgcaacgag gatttttttc cacaggggac
      961 gttttgtagt ttcggtggta cgctcattgc cgagcgaacc gggtaacgac cggataattg
     1021 gcccggttgc ggcgtctttc gagtcgcgac cagtgttgaa gcggggtgat gcggcttttt
     1081 tccctgagcc actgcggctc gacgcagggc tggatttccg ggcagggcca cctttttttg
     1141 ccatagggcg gtaaaaaagg gacaaaaaca cattgcgtaa agcggataga gtccacgcag
     1201 cttacctacg ggtaagctgc gtggacaaat gaagatcgag tttttggctt gtcgcattat
     1261 gtctcatcag actgatgcga cataatatgg cagtagaagc ggcagtctcc acccagtatg
     1321 ttcctgtaac gggcgccgaa aagtcttcgc gcaaccagtc taagggcgcg atacagtacc
     1381 tgcggcccac gctctcgcgc aacgaactga agtcagtgct cgaaagcctc gtgatggaag
     1441 aggtgagctt cggccagggc gtgctcaact tcgaaaaagc cttcgcgcgc acctttgatt
     1501 ttcagcacgc agccgcagta gattcatata cctccgcatt ccacctggcc ttgctcgcct
     1561 gcgagctcaa taaagaagac gaaattattg tgccggtctc tgcgccgctc gcgttctttg
     1621 acgccgtgaa ctatgtggga gcgaaagttg tcctggtaga tctcgcgaaa gactcattcc
     1681 actgcgacat tgaccaactt ttcgccgcgg tcactgaaaa gaccaaagct gttttcctgc
     1741 cgtattcttt tggttcatac aaagacttcc gtccattcta cgaaaaactc gaggcggccg
     1801 gactgcgcaa aggtaaagaa cagcgcgttc gcgtgatcga agatatctcc cacgtggttg
     1861 gccacgacta taacggccgc caggcgggca gcgatgccga tatcgcgatt gcaggtctca
     1921 acgaagacca cctgatgacc atcggtaagg gtggcgtcgt gatgaccgac tccaataacc
     1981 tgtttgccct catcaaagat ctgcgtgtgc agggcggtaa ccgcccgtac cgcgtgcgct
     2041 ttgactacgc tatcaccgat taccaggccg cgatgggtat ggagcagctg ggcaacctcg
     2101 ctgcgattat cgagcgccgc cgccgtatgg gcgtgcttta taatgaggca attgcttcga
     2161 gccggctgaa aacctatttc gtcgcacccg aggcagatgt ctgcggggct ttcccggtga
     2221 tcggcgaggc ggagatcggt catatgcagc gctatttttc gtcgctgcag atcgaaacgc
     2281 ggcgcgtttt tcctaacggg ccactgcacc acatcatggg acttaatccg ctcgagtacc
     2341 cgaacgcgga aaaaatttac cagcgtggct tgttgctacc gctttatccc atgctgaaca
     2401 aggctgcggt agaaaaaatt actgcagcaa ttcgcggctt ttattaagag gccgcgaaaa
     2461 aaaatcagcg cattaagtca catccttgca agtcgcgtgc cattttttga gtaagaatta
     2521 ttagcacatt gtggaattct gtgcataact tttcgctgca caagtgacat attaattctg
     2581 cgcgcggaaa tttttttcta aaaaattctg atttcgtctt tacgttgcgt gccgcagatt
     2641 gagacgctca cggatcctct gattcgatcg gggcaagcgc atttcatgtc agacaagcag
     2701 cagaaattct accaatgccc cgaatgcact tcgatcgtcg aacgcgtcta ccagaacggc
     2761 gtctgcaaga gttgcctgag caagaagttt caggcggtgc gattggtcat cgaccataac
     2821 cacgctctgc aggacgcaca ggcaaaggtt ggccgcgtct cgcgcgcact cagccgatga
     2881 acgctgcttc atcgtattcc gtcattctcg atcgcgacaa gactatcaac caggatcccg
     2941 gttatctcgc cgacccggcc gccgtcgtcc tgatgcccgg ggcagctgaa ggcatcgcgc
     3001 tgctgaatca tttcggtatc agggtcttcg tcgcttctaa ccagtcaggt gtgtcgcgcg
     3061 gcctgatcag cccgcagcag ctgaaagccg tcaacgcgcg tatcgaagag ttgctgcgcg
     3121 aaggtggggc ccatatcggc ggtttcttca tttgcccgca ccaggaccac gaagaatgcc
     3181 attgccgcaa gccgaagcct ggcctggtga agcagatcat cgagacgcac cgtatcgatg
     3241 tcgagcgcac atttatcgtc ggcgatcgcg cgcgcgacct cgaatgcggc cagtcttttg
     3301 gtattcgcgg tatcctggta ggcgatgaag aaacccagcg cccgcagaac cttgttttcc
     3361 aggcgaataa cctgcacgaa gcagcggcat atattctcga aacgatcttt gaagagcaga
     3421 cgcagaagaa gtttttcgcc tcggtacacg ccttcatgcc cgaactgcac aagcagcgcg
     3481 ctgcaggccg gcggattgtt ttcactaacg gctgttttga cctgctgcac agcggccacg
     3541 tgcagctgct ttcacaggct cgcgcgctgg gtgactatct ggtgctcggg ctcaactctg
     3601 accgcagtgt cagcgcgctc aaagggccca cccggccggt gaatagcgag accgaccgtg
     3661 gccgtatact ggcgcaatta ccctacatcg atgcggtggt ggtgttcgac gaagatacgc
     3721 cggttgaaat gatgaaaatt atccagcccg atatccaggt caagggcggc gactacgtca
     3781 aagaaaagct gccggagttt ccggtgatgc aggcgatggg caaagagatt gttattcttc
     3841 cgtttagaaa aggttattca acaacgggaa ttctggctaa atcgaatcaa cgggctcctt
     3901 aggagcccgt tgattcgaca tttgccttca cttcgccttt agcgagtcgt acccgtattg
     3961 tttcaccgtg tttcagcgct gccgcatcgg tgacgatctt gtctcccgcg tagacaaccg
     4021 aatatccccg ccgcaacgtg gcgagcggtg aatatgcctc gaagcggccg gaagttgatt
     4081 cgaattcagc gcgcgtcagg cgcagtttct gctgcacgag tacgccgagc ggagtcagct
     4141 ggttcagaga attggcagcg gtatgtacca tgttgcgaaa agtaaaatgt aactttgatt
     4201 cgagttcgtc cagccagcgc gactgctctt cgacgagcac gtaaggtttt tcccagaatt
     4261 ctttttctgc cgtgcgcaag aaacgttcct gcattacgcg cagctccgcc tctaccgcgg
     4321 catacgcgcg tctgagtagt tgctccgact ggttcttgag ttcatcaacc acggggaaaa
     4381 cctgccgcgc cgcatcggtt ggggtcgctg cagcgtagtc ggcgacgtcg tctgaaatcg
     4441 ggtgatcgat ctggtgaccg acggcagaga caaccggcac gcggcacgtg gcgattgccc
     4501 ggcacactgc ttcatcattg aacgccatca ggtcttcggc cgaaccgccg ccgcggccga
     4561 cgataatcac ctcgcatcgc cactcaggtt tctggatttc tttcagggcg gcgacgatcg
     4621 attgcggtgc ggcttcgccc tgtacctgcg caggcgcgag cacaatctcg gcgttagggt
     4681 aacgctcctg cacctgtttg agcatgtcgc ggtacgctgc agtacctgcc ccagtgacaa
     4741 tgccgacgcg ctttggcaaa agcggcagtg cacgccggtt gtctgtcctg atcaggcctt
     4801 ctttaagcag tttttcgcgg atcaactgca ggcgctgcag caggtcaccc gcgccgatct
     4861 tctggacctt cttcaccgtc aggctgtatg tcgactgcgg cgcatagatg ccggtggagc
     4921 cgagtacttc gacctcgatg cccgccttga gttcgaagtc gagcgcggtt tgcgcgaaac
     4981 gaaagtaggt gcagcggatc gtaggtcggg ggttggcgcc cttggtcgac gtgtcagaca
     5041 gagtcatgta gatgtgcccc gaagaagcat gcttctggta tgacgtgatc tctcccttta
     5101 cccagacatc ctgtagttcg ggggagttct taatcttgta ttcaatgacc cggcaaagtt
     5161 cggctacggt gtactgctgc ggcactgcgg gataccaaca ggaaattgac gcacgtcgtc
     5221 aatttcctgt tggtgtgtat gcaatcttac ctgatcacac aatgcctgca gaacgatttc
     5281 gtccggctgc tcgataggta cgatccgctg ccgaactacc tgcacatcgg ctacgacgag
     5341 gcaaaccgcc tgctcggtga acgcgttgaa gaaggcccgg tggcgcagat tatccgctgg
     5401 gcgtacaatg aaactcccga ccgcctgcag attgtgcaca tacgcgactg gcacgatgcg
     5461 gccgacgccg agcagcaggc acaccttgcg cagttcgggg cgcactgcct gaagggtagc
     5521 gagggcgcgg agtttgtctt taaccggcag gttgtgccgg gccgcaaggc ggccatcgtt
     5581 gatgcctcgg gcctcaacga cttctacaag accgaccttg ccggctacct gtcaccctgt
     5641 cttgaagcgc caacccgcat cggcctgatc ggtgtctgga ccgaggccaa gatcacctac
     5701 ctggcgtacg agctgacaac acgttacccg aaggcagaga ttatcacgtg cagtgctctc
     5761 acagcgagtt cgtcgcgcag ccagcacttc atcgcgctcg atcacctcag gaaaatcctg
     5821 ggtgtcaaag tttttgattc cgtcgccagt tttgccgaat acctcggtgg ctcaggccat
     5881 ggcctgacgg cctctgcgta tgcgcagaat aagcagctcg atgacacgaa attccgtttc
     5941 aacataccgt acgagcttaa agaagcagac aacaaaatac tgcactatct cttccgtgac
     6001 agcaaatcgg cagactttat ctgcctcgac ggcggtttca gcggcaacgt cgtactcaag
     6061 gcagagtcgc gcgatgcgta cgggcagaag cagcaaccca cagtcgtcaa gatcggccag
     6121 cgtgacccga tttcgcgcga gcgcgcctcg tttgagcgca tacaagaagt gctcggcaac
     6181 agcgcgccgg cagttgtcga ctttgctgaa tttggcgagc gcggcggcat caagtaccgt
     6241 tacgcggcca tgaccgatgg caaggtcagg actttccaga aatactttgc cgaaaccgat
     6301 gacgtcgaat tgctcaagaa gtttttgcag gtggtattca ggcgacagct cgggcgtttc
     6361 tacgaagcgg cctcgcagga aaatctcaac ctgcttgagt attacgactt ccggtcaaag
     6421 tacgccgact ccgtcaggaa aaaatgcgca gaactgtcag agctccgccc cgatggcagg
     6481 ctgcagatcg cgcccggcat cgacgtcgaa gatgtctcgc gtttttatgc cgaagacctg
     6541 cagcacctgc gcgagacggt cgcggtgccg cgttatgttt cgtggctgca tggcgacctc
     6601 aacggtgcga acatcatcat cgacgaatcg cagaatgtct ggctgattga tttttttcac
     6661 acacaccgcg gccacgcgct gaaagacctg atcaaactgg agaacgacat tctttacatt
     6721 ttcacgaaga tcgaaaatgc cggcgacctg cgcctggcct gcctggcaac agactggctc
     6781 tatgcggtgc acgacctcgc ggtaccgcca caggatatgc ccgcagagct tgctgccgac
     6841 aaacggtttg ccaagtcgct cgcggtgatc tgctacctgc gcagccttta ccgcggtatc
     6901 atcgacctcg accgcgatcc ttaccagttt tttgtcgccg cggcacgcta cgcgatgcac
     6961 acgctttcgt tcgacgaatg caacctgtgg cagcgcaagt gggcgctcta ttcgggggca
     7021 aaggcgcttg cggcagtgcg cgacgcactc acgaagactg cgcgcctgcg aattgatttt
     7081 atgacggtag ccggtgaacc ggcgagcgga gaaccatcag ctaccggcct tctgggtatg
     7141 acgattttgc ccggtcgcaa agaccgcggt cgggacctgt cggctgatct ggacgagatg
     7201 aagaagcaga aggtggcgca tgtcgcgacg ctgatcagca ataacgagtt cggggaatac
     7261 ggggtgggca acctgctcga agcctataaa gcagcaggtt tcgactttat ccaccgcgcg
     7321 gtaaaagacc agggcgtgcc gacgcggcag caggcagaag aagtgctggt ctggatcaaa
     7381 gaacgaaccg accgcggtga gaatgtcgcc atccactgcg tcggcggcct cggccgtacg
     7441 ggcatcgtcg ccgcactgta tctcaagaag tatgcgggcc tgaaggctga gcaggcgatt
     7501 gagcgcgtgc gccaggtgcg ttcgccgcgc gccgtcgaaa accaggagca ggaaaatttt
     7561 attaaggact tctgagtttc gcttttcgca gctaggcgtg aagccaacgt atcccgatgc
     7621 ctgaaagtac aattgctcgt tacgatgtgg tggtgatcgg gtcggggccg gccggtgaag
     7681 gtgctgcgat gcagtgcgcc aaggacggca aatcggtcgc gatcatcgag ctcaatgcgc
     7741 aactgggggg tgaatgcctg caccggggca cgataccttc aaaggcgctg cggcattcga
     7801 tacagcgcta catcgaggcg atgtcgaacc cgctcgttaa gggacacttc agcggccgcg
     7861 atctcgagtt tccgcaggtg atggccgcgg cccgccgcat catcgaccgc gaggccgacc
     7921 ataacctcgg ctactacgag cgtaaccgcg tgaagattta cggcggccgc gcacgcatca
     7981 ctgctcctca ccgcatcgaa gttgacctcg gtcgcgccgg aacggaaacc atcgaagccg
     8041 acaacatcat cattggtacc ggttcacgcc cttaccgccc ggcgaatgta ccctttgacg
     8101 accgcatact cgacagcgac accgtactcg atctcgctca cactccccgt tcgctgacga
     8161 tctacggcgc gggagttatt ggctgcgagt acgcgtcgat cttcaagggc ctcggtatta
     8221 aagtgacgct gattaaccac cgcgccaagt tacttgagtt tctcgacgat gaaattatcg
     8281 atgcgctgag ttaccacctg cgcgagcagg gcgtggttat caagcacggc gaagaatacg
     8341 agaaggtcga gcgcgcaggt gatgaagtta tcctgacgct caaatcggga aaaattatta
     8401 agtcagaagc tttgctctgg gccaacggtc gcagcggcaa taccaacgga ctcttcggct
     8461 tcgaaagcgg catcgagatc gatcaccgcg gccaggttgt gaagaacgaa aactttcaga
     8521 cggcgttgcc acacatctac gccgtgggcg atgtgtcagg cccacctgcg ctcgcaagcg
     8581 ctgcatttag ccagggtcgc ttcgccgcat tgcacatcac gcgtggtatg gcaatcggtg
     8641 gtctcgtgcg cggtattccg acgggtatct ataccaaccc cgaaatcagt tcgctgggcc
     8701 agacggagcg cgaattgacg gcgcagaaaa ttccgtatga agtcggccag gcacactttc
     8761 gtaatattgc ccgtgcgcag atcacggggc agacggcggg cctgctgaag atactctttc
     8821 accgtgagtc actggagatt ctcggcatcc actgttttgg cgaccaggca tcggaaatca
     8881 tacatatcgg gcaggcgatc atgtcgcagc cgtcgccgca caaccgcatc acctggtttg
     8941 tcgagacgac attcaactac ccgacgatgg cagaagccta ccgagtcgcg gcgttgaacg
     9001 ggctcaatcg cctctgatct caccgctccc ggttagtgtg aattaacctg aggttggcgt
     9061 ccattttgtc ggggtggcgc cagagacgca tttctttctg gtagagccgc gccacctgcg
     9121 cacgccgtat cttgagggtc ggtgtcagtt cgcggccgcg gtgaaaaagg tgtggtacga
     9181 gggaaaatcc cactggccac gcataggcag gcaaacccga gtcacccaag agccgggtga
     9241 attcgcgtgc atacaggctg cgcacggcca cactctgcgt gacgctctgc cagaattttt
     9301 tgtccgattc gggttcgtcg gcgagtttag cgtcatgccc gccgtgcggt tcgagccagg
     9361 ccagcagcgc cacgaagtca ggcacgacga gcgcaaaaag cggattcgta ccgccgccga
     9421 gcaccaccac cctgccgacg aagcgattac cgcgcagtac gttttcgacc agctcgggaa
     9481 acaccagctc gccgttcgcc agctgcagta cattcgcctt acgacccacc acgatgaggt
     9541 tgcgcgcatt gtcgacgtag ccgatgtcgc cggtaaggta agtgggagtt gctgcggtga
     9601 cgctgcccag tgcagtgaat gatgaaaaat atccggggaa aacattgtcg ccgctcacgg
     9661 caatttcgcc tagagtcatg ccattccatt cacaagccag ggtggaataa atggccactt
     9721 cgacaccggg tagcacgcga ccgacaactc cgggaaagtg cctgccgggc ctgcgcagcg
     9781 cggtaacgca ttgttctgtt gaaccgtaac cctcaagaac ttcgaagccc agcgcgcgca
     9841 gcgtttcgtc gactgcgagc ggcaacgggg catcgcccat gatggcgtag cgcagtgtcg
     9901 ggctgaccct gcggcgtatc gcggcgaaca gagtcgccat aagggccgtg cgtaccggca
     9961 gcaggtaggc gagctgcagc caggcaaata tgcttcggtt acttttcgcg tagcgcgagc
    10021 ggcttgccgt actctcgaag atgcggttcg tgtgcaccga gagcctgatc aggcgccgca
    10081 gcattttacc gacaaaacct tgtcccaact gcacgagaat ctcgtggtga attttctccc
    10141 agatttcggg aaaactcgga aagatagtac agcccgatgt cctgagcgcc tgctgcaggc
    10201 catgaatcgg cgcatagaca atctgcgctc cgcatgccag cgctgtatat tcgaccagtc
    10261 ttgcgtacaa gtgccacgaa ggcaggcacg agagcaccac gtctctaggc cccatgcgca
    10321 gcacgcgtgg tatgacacgc atcgagtgca gaaaagacgc gtgcagcacc ggcacctgtt
    10381 taggagttcc ggttgtgccc gaggttctga tcactgccga gacagccgaa ccctcgtggt
    10441 gaaaagccac ggcctgtgtt tccctcagaa tcgtttcgag cgcagtaata tttttgtggg
    10501 ctggaggcac gcccgagagc tggatgcagt gtgcggacgc gagcagggcg cgtatgtggc
    10561 gggtgcctgc tgcatctgca gtaaaaaagt gccggcagcg cgtcgattta accagcgaag
    10621 cgatttccct gctgccggcc agcgggctca gcggaaactc caccgagccc acggcctgca
    10681 ccgccagcgc cacgagcaac cagagtggat ggttgtctgc aagcacgatg accgcggcgc
    10741 cttttttgag atgcaggcgc tgttgaagaa acgccgccag ccggtcgacc tgctgtgcta
    10801 gttcgcggta ggttagcggg ccctgtggcg tctgc
//
LOCUS       DAOQJF010000075        10812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3136_length_10812_cov_5.437854, whole genome shotgun sequence.
ACCESSION   DAOQJF010000075 DAOQJF010000000
VERSION     DAOQJF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3136_length_10812_cov_5.437854"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..806
                     /locus_tag="PLF85_05745"
     CDS             129..806
                     /locus_tag="PLF85_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE19198.1"
                     /translation="MGRKTLDKERDLDPKVRSEYLARLLPFFIEKGMSVHSMDDIVQF
                     LEISKATFYRHFRSRDELFELFIEYVVDQILSSRYFLYDQNLRYEERLLTTFGAILQQ
                     INGIGFLLLADLRTNLPHLWEKVRETYAVWEKELHKFFIEGIERGFVHDVNPAILAHM
                     VVVFSRELMRPEYLQSLNMTLAEAFGEMFKIQVRSIVRNPEFSPEALDERMGSMLPEI
                     KAQLLKD"
     gene            complement(929..1276)
                     /gene="rplT"
                     /locus_tag="PLF85_05750"
     CDS             complement(929..1276)
                     /gene="rplT"
                     /locus_tag="PLF85_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNE19199.1"
                     /translation="MRSTNGTIHKNRRKKILKRTKGFRAGRRRLYRTAKDAALKADMW
                     AFRDRKQRKRHFRALWISRINAACREEGISYSKFIAGVLKAKVGLNRKSLSELALNDP
                     AAFKEVVRVAKAA"
     gene            complement(1286..1483)
                     /gene="rpmI"
                     /locus_tag="PLF85_05755"
     CDS             complement(1286..1483)
                     /gene="rpmI"
                     /locus_tag="PLF85_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNE19200.1"
                     /translation="MHGKKTNKAAKKRFRVTATGKIKRTKAGKNHILTKKTAKRKNDL
                     AKAGYVHEADMGRVERLLGIK"
     gene            1669..2307
                     /locus_tag="PLF85_05760"
     CDS             1669..2307
                     /locus_tag="PLF85_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNE19201.1"
                     /translation="MKLIKRYANRRLYDPETSRTITLDEVAQYVRDGIDIKVVDNTNG
                     EDITRKVLGQTFLKLNENDHVANPVLQNQMLKLLIRESKGGVLEVLKKLVLAGVGMTQ
                     STSEERKTLLEMLINPSEAHEQEQRAFLKGLADKGQQEADRVLQSISGLVSNISQSIQ
                     DNMVSVLDPNERARKLDHLLGQIESIQKSISKFSKTGETTETEEPKSGAKAG"
     gene            2308..3045
                     /locus_tag="PLF85_05765"
     CDS             2308..3045
                     /locus_tag="PLF85_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE19202.1"
                     /translation="MPVIIPPPRHSLGSRLLRLLLLSLLVAGSILFVCYRFGETPTEK
                     AERLFTQRRLAELKSYTQKRLGAGEASPLLMSYYIAAEFSSNAQASLESLLSNLRALD
                     DRPIFRRETLQRLIQIPENRKRLGEILAAALVIEKPPGNETLALIRSILESDADLSGS
                     SADFAALAEIFPRQVRLVSAQKLQLRSGPTTDSEVLRRLEDGERLLLRYSGPAVLVSG
                     KRGRWVFTLDAQMVSGWVFDAYLAPEK"
     gene            3119..3922
                     /gene="trpA"
                     /locus_tag="PLF85_05770"
     CDS             3119..3922
                     /gene="trpA"
                     /locus_tag="PLF85_05770"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNE19203.1"
                     /translation="MHPFTRLMTERKKQKGYLFIPYIAYGDPDSKITFEIISKMIEGG
                     AATVEIGLPFTDPVADGPALERAFKRSLKHEFHISDVLSMLQKLHKKYPAFPFTIMSY
                     ANIFYQAGFKNLLEKLYECNVQGVIIPDIPFDEKKYIIEKEGLADILSRVAWIDFITP
                     TTSLKRVQEVAREANGFIYLVSYKGVTGQKNFTLKPLKPLIKGVRKATKVPVVVGFGI
                     RDKSHATEAIRMADGFIMGTVFHEMIEHNIERAPAIPHEIGWALPNMLP"
     gene            4105..4665
                     /locus_tag="PLF85_05775"
     CDS             4105..4665
                     /locus_tag="PLF85_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE19204.1"
                     /translation="MQLESARLMALDGFHVFVPDLFSEEGAARYCLRMFFNMAFVTNE
                     ADNPGVREVFEIIDQVKTMPGVDAARIGLIGQCLTGGFALHAALRDDVKAPVVFHHSL
                     GMTGSGMPADSARAVCKPVQGHFVQIDPMCPKSRVEQLKEQVGTFLEVNYYDWLPHGI
                     PHFFRRTDEGRRAYINMMRFLKMQLA"
     gene            4740..6749
                     /locus_tag="PLF85_05780"
     CDS             4740..6749
                     /locus_tag="PLF85_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HNE19205.1"
                     /translation="MERAAHKIYNKEGNPVREEPWIFRTYGGHSNARATNELYRTGLA
                     RGQTGLSIAFDLATQCGYDSDHAMARPEVGKVGVPINSLADFRILFDQIPIEKMNTSM
                     TINGTAMWLLSLYIALAEERGVDPALLQGTTQNDIIKEYLARGTYIFPPESSIRLIVD
                     MYEYCVRAIPKWNPSNICSYHLQEAGATPVQELSFALVTAIGILDAIKARNCFSEAEF
                     EQAVGRISFFVNSGMRFVEEMCKLRAFGEMWDEITRDRYGVKTEKFRRFRYGVQVNSL
                     GLTEEQPENNAWRILIEALGVTISQKARCRALQLPAWNEALSLPRPWDQQWSLRLQQI
                     LAYETDLLEYPDLFDGSPVIQSKVNSLITEAKAEIDKVLAQGGAVEAIKSGYMKSQLV
                     KSQAERMSRIMTGELKVVGRNIYADGLPSPLLGGADSGIFKIEHSAADETLDNLKKVR
                     ESRKAAEAEAALKQLEDDARGNVNLMPASIRCAKAMVTTGEWANTLRKVFGEYRPLTG
                     VEGQKLSLSEDRVATVRKKIEAFKEKHGHRPRFVIGKPGLDGHSNGAEMIAVAARHVG
                     FDVIYSGIRLTPEEIGRAAVEENADLVSMSILSGSHLELVDALMKTLAQHKADKEIPL
                     VVGGIIPESDFARLKAMGVQHIFTPKDYDLVAIMETNLEIAEKFA"
     gene            6842..9133
                     /locus_tag="PLF85_05785"
     CDS             6842..9133
                     /locus_tag="PLF85_05785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19206.1"
                     /translation="MPSAPIQGLAEAYVPRRLLAFYTRMVRLLTPGAFLVLLTLPVVP
                     VLNYYGYLFISGWQSQPMSQLYGAELSSCWPVFVLSAAVFVVSGGGLARLGLPSVLPV
                     IRRLSRSVRLLGGEAPADELRQLLRDLVRAPAAFTWLAVAAYTLQEVLFYWQSYAASG
                     TTNLPLFLTVDLFLYVILIGLVYSITDFALAFLRMQVKQHLAAYGESSNDLVSGGPGS
                     YRLLYLFFVGAVATFALVAFLQSRNNHVVSEAIQFAAITFMICGSLAFVYFFTWSYSL
                     KQLNAATYSVAMGGRGHLPMLSNDREMVELAVNFDAATYEINTIRNYLTELVDEKTHT
                     LSEAYNELKQLKSRQDGDYYLTSNLIESLTEIRPESETVVIDSLVKQFKVFEFMGHSK
                     EIGGDMNAGYALRLQGEKYTLLLNADAMGKSLQGAGGVLVLGSALRSLIERTHITEGL
                     RSLSPERWLKNSFLELHRLFSSFNGSMMASMACLLIHDNSGYTVMVNAEHPHGILLRG
                     RDAQFISNHEGLRKLGNNAVKGRLWVDTLLLHPGDVVILGSDGRDDIIVGYDGDGQPI
                     INDDEDRILQIVKAGEGDLPRIYMALKQSGEIVDDVSLLRVQYTGRQSKVDQQAVTEL
                     WRQFGQAKENAGRLGILRRVLHEDADNMKAWRALLRIHLHNKNLIDAAFIAECMVRIR
                     PERDFLMYSCALLYFRNGNLEKALNFADRLTLRNWQVERYLVLLGRIHEARQDAAGMR
                     EAGLLLTELDGSHPYGKKLLNLP"
     gene            complement(9130..10047)
                     /locus_tag="PLF85_05790"
     CDS             complement(9130..10047)
                     /locus_tag="PLF85_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19207.1"
                     /translation="MTSQLLRNQAIRAARNYFNEHGLLEVRTPKCVSAGAMEPYIDTF
                     ALQSSISGFVGYLASSPEFAMKKIFAAELQHNPGARGIYEIAPVFRDDRAGKLHQPEF
                     TMVEWYVQDIQRQDIITMAVELIRYVAATVGKTIAPPQVTNVDLREVLVEQSFSPDFN
                     QPQPYTRLYGTRFGRVPQHFNHLDGEIACFNLLFDEVVQPVIREMPGIVAVEGFPDCL
                     AALARSDGKTAARTELFISGVELANAYSEEFDANVIRQRWSAYNEIRQLRGVPEHPVD
                     EELLETASAMRSASGIALGLERLLLMLPA"
     gene            complement(10044..10709)
                     /locus_tag="PLF85_05795"
     CDS             complement(10044..10709)
                     /locus_tag="PLF85_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNE19208.1"
                     /translation="MPGTPHATLPEIIYVFDGYCGWCWGISEVVTRLARDFSDRFRFS
                     ALSGGLITGERIGPLGDFSAYIEKAIPRVAQMTGAVFSEAYVARIRDRTTQQDSRVPA
                     AVFAAVLAAKPGTDTVQLAHDILVLNFTKGRDMSRYDEYAGLLTAYGLDAKQTLSELA
                     ENRYLPGAEQQFTFARDIGAEAFPTIVYGRDGQYFPLCQGYQSYENLAHALDVLHKEP
                     PEL"
BASE COUNT         2360 a         2958 c         3059 g         2435 t
ORIGIN      
        1 ttcccgctca cggggcagga gttatttttt gttgccatac tgatcgtcat gagtgtgctt
       61 gttgccatgg tgcctgcata ccttgtttac cgccgtgcgc tgtcagaggg cctgcaggtc
      121 aggcagtaat gggacgtaag acactcgaca aagaacgcga cctcgacccg aaggttcgca
      181 gcgaatatct ggcgcggctg ctacccttct ttatcgaaaa gggcatgagc gtccacagca
      241 tggacgatat cgtgcagttt ctcgaaatta gcaaggctac cttttaccgg cattttcgct
      301 cacgcgacga actttttgaa ctgttcattg agtacgtggt cgaccagatt ctttcgtcgc
      361 gctatttcct ctacgaccag aacctgcgct acgaagagcg gctgctgacc acgttcggcg
      421 ccattttgca gcagatcaac ggtatcggtt ttctgctgct ggccgacctg cggacgaacc
      481 tgccacacct gtgggagaaa gtacgcgaga cctatgcagt ctgggagaaa gagctccaca
      541 agttttttat tgaaggtatc gagcgtggtt ttgtgcacga cgtgaacccg gcgatactgg
      601 cgcacatggt ggtggtgttc tcacgcgagc tcatgcggcc cgaatacctg cagagtctca
      661 acatgacact cgcagaagcc ttcggcgaaa tgttcaagat ccaggtgcgc agtatcgtgc
      721 gcaacccgga attcagcccc gaggcattag acgagcgcat ggggtcgatg ctgcccgaaa
      781 tcaaagcaca actgctgaaa gattaaaaaa accggagcct accggcgctc tctatcgccc
      841 gtaagctcca gccaaatcaa tctgtaagga ggaaattaac gaaaaagaaa atagcaggcg
      901 gcccttgcgg gccgcgttgg ggatgctgtt acgccgcttt cgcgacgcgc acaacttctt
      961 tgaatgcagc tggatcgttc agcgccagtt cgctaaggct cttgcggttc aggccaactt
     1021 ttgccttcag aacgccagcg atgaacttgc tgtagctgat accttcttcg cggcatgcag
     1081 cgttaatacg tgaaatccac agtgcgcgga aatggcgctt cctttgttta cggtcgcgaa
     1141 acgcccacat gtctgccttg agcgcagcgt ctttcgctgt gcggtaaagg cgacggcggc
     1201 cggcgcgaaa gcctttcgtc cgtttaagga tcttttttct gcggtttttg tgtatggttc
     1261 cgtttgtcga gcgcatggtg ttcccttact taatgccgag caggcgctca acgcgaccca
     1321 tatcggcttc gtgcacgtaa ccggcctttg cgaggtcgtt cttgcgctta gcagtttttt
     1381 ttgtcaggat gtggtttttt cccgctttgg tacgcttaat cttacctgta gcggtaacgc
     1441 gaaaacgctt cttcgcggcc ttgttggtct ttttgccgtg cattgtccag actaggtttt
     1501 tcagacatag cgtcaaaccg aatgaggtgt gaagcctgct cgggggtacc ccatgggata
     1561 aattgtatca gggcgcttaa accttgacgc agcgtctcaa aaatcgtgca ttgcaccaga
     1621 aattagcagt ttcgggcatt ttgcacgccg cactgcacta aggaataaat gaaactcatt
     1681 aaacgctacg ccaaccgccg gctctatgat cccgaaacct cgaggacgat taccctcgac
     1741 gaggtcgcgc agtatgtccg cgatggcatc gacatcaagg ttgtcgataa caccaacggc
     1801 gaagacatta cccgcaaggt gcttggccag acattcctca agctgaatga aaatgaccac
     1861 gtggccaacc ctgtgctgca gaaccagatg ctcaagctcc tgattcgtga aagcaaaggg
     1921 ggagttctcg aagttctgaa aaaactcgtt ctggcaggcg tcggcatgac gcagagtacc
     1981 tctgaggagc gtaaaaccct gctcgagatg ctgattaacc catccgaggc gcacgagcag
     2041 gagcaacgcg cattcctgaa gggcctggcc gacaagggcc agcaggaagc cgacagggtg
     2101 ctgcagagca tttcaggcct tgtttcgaat atttcacagt caatccagga taacatggtt
     2161 tctgtgctcg acccgaacga acgcgcgcgc aaactcgacc acctgctcgg gcagattgaa
     2221 tccatccaga agtccatcag caagttcagc aagacgggcg agactaccga aaccgaagaa
     2281 cctaaaagcg gggcaaaggc cggctaaatg ccggttatca taccgccgcc gcgtcacagc
     2341 ctgggcagca ggctgctcag gttattgctg ctatcgctgc tcgtcgccgg cagtatactg
     2401 tttgtctgtt accggttcgg ggaaactccc acggagaagg ccgagcggct ttttacacag
     2461 cgccggcttg cagaactaaa atcttatacg cagaagcggc tgggcgcggg cgaggcaagc
     2521 ccgctgctga tgagctacta cattgccgct gagttcagca gtaatgcaca ggcaagtctt
     2581 gaatcattac tttctaacct gcgggcgctc gacgaccggc cgatctttcg ccgcgaaacc
     2641 ctgcagcgtc tgatacagat tccggagaac cgcaagcggc tgggcgaaat actcgctgca
     2701 gctttggtta tcgaaaaacc gccgggcaac gagacgctcg cgctgatccg cagtatcctt
     2761 gagtccgacg cagacctttc aggcagcagt gcagactttg cggcgctcgc cgaaattttc
     2821 ccccggcagg tacggctggt ttcggcgcag aagctgcagc tgcgttcggg tccgacaacc
     2881 gattctgaag ttctgcgccg gctcgaagac ggcgagcggc tactgctgcg ctactctggt
     2941 ccggcggtgc tcgtttcggg caagcgtggc cgctgggttt tcaccctcga tgcacagatg
     3001 gtatcgggct gggtgttcga cgcctacctg gctccagaaa aatagtgcat tgcacaacat
     3061 ccggagtgag tgccactttt tttgcttgtc gccgcttggc ttcggccctg agtacactat
     3121 gcatccgttc acgcgcctga tgaccgagcg caagaagcaa aagggatacc tgtttattcc
     3181 ttacattgcg tatggtgacc cggacagcaa gatcacgttc gaaatcattt cgaagatgat
     3241 tgaaggtggt gcagcgacgg tcgaaatcgg cttgccgttc actgacccgg tggctgacgg
     3301 ccctgcatta gagcgcgcat tcaaacgttc gctcaaacac gagttccaca tatctgatgt
     3361 gctgtcgatg ttgcagaagt tacacaagaa atatcccgcg tttcccttca cgatcatgag
     3421 ttatgccaac atcttctacc aggctggctt caaaaacctg ctcgagaagc tgtacgagtg
     3481 caatgtgcag ggcgtgatta ttcccgatat cccgttcgac gagaagaaat acattattga
     3541 aaaagaagga ctggcagata tcctgagccg cgtcgcctgg attgacttca tcactcccac
     3601 aaccagcctg aagcgcgtgc aggaagtcgc gcgcgaagcc aatggcttca tctaccttgt
     3661 gtcatacaag ggtgtgacgg ggcagaaaaa ctttacgctc aaaccgctga agccgctgat
     3721 caagggtgtg cgcaaggcga ccaaggtccc ggtggttgtc ggcttcggca taagagacaa
     3781 gtcgcacgcc accgaggcga tacgcatggc tgacggcttt atcatgggaa ctgtgtttca
     3841 cgagatgata gagcacaata tcgagcgtgc cccggcgata ccgcatgaaa tcggctgggc
     3901 gctacctaac atgctgccgt aagcagacat gtaaaccgct aactagcaat cgacgcgctg
     3961 tctgagcgtc cactttgggc aactatgaaa gaagtccgca agttcagcct cagcaaccgc
     4021 gatatcaatg ttcatatttt ccgtggcggc gatgaaaatt tgcccgcggt gatctttttg
     4081 ccagacctca ccggggtggg ctaggtgcag ctcgaatcgg cgcgcctgat ggctctcgac
     4141 ggctttcatg tttttgtgcc cgatctgttt agcgaagaag gtgccgcccg ctattgcctg
     4201 cggatgttct tcaacatggc atttgtcacc aacgaggcgg ataatcccgg tgtgcgcgaa
     4261 gtctttgaaa tcatcgacca ggtaaagact atgccgggtg tagatgccgc gcgcatcggg
     4321 ctcatcggcc agtgcctgac cggtggcttt gcgctgcatg cggcgctgcg tgacgatgtg
     4381 aaggccccgg tggtgttcca ccacagtctg ggcatgacgg gcagcggtat gccggcggac
     4441 agcgcccgcg cagtctgcaa acccgtgcag gggcacttcg tgcagattga cccgatgtgt
     4501 ccgaaaagcc gggtcgagca gctcaaagag caggtgggta cttttctcga ggtcaattac
     4561 tacgactggc tgccgcacgg cattccgcat tttttccgcc gcaccgacga gggccggcgt
     4621 gcgtatatca atatgatgcg ctttctcaaa atgcaattgg cttaagaatg gtatgccctg
     4681 tgggcatacc attcttaagc tacgtttgac acagcgtttc gcaatccggg tttaacggta
     4741 tggaacgcgc agcgcacaag atctacaata aagaaggtaa cccggtacgc gaagaaccgt
     4801 ggattttccg cacctatggc gggcactcga atgcgcgtgc caccaacgag ctctaccgca
     4861 cgggcctggc gcggggccag acgggtcttt cgatcgcctt cgaccttgcg acccagtgcg
     4921 gttacgattc agaccacgcc atggcgcggc ctgaagtcgg caaagtggga gttcccatca
     4981 attcgctcgc cgatttccgc atactctttg accagattcc catcgagaag atgaacacct
     5041 cgatgacaat caacggtaca gcaatgtggc tgctgtcgct ctacattgcc cttgccgaag
     5101 aacgtggcgt tgacccggcg ctgctgcagg gcacgaccca gaacgatatt attaaagagt
     5161 acctcgcgcg cggcacgtac atttttccac ccgagtcatc gattcgcctg atcgtcgaca
     5221 tgtacgaata ctgcgtgcgc gcgattccaa aatggaaccc atcgaacatc tgctcctacc
     5281 acctgcagga agccggcgcg acgccggtgc aggagctctc gtttgccctc gtgacagcga
     5341 tcggcattct cgacgctatt aaggctcgta attgcttcag cgaggccgag ttcgagcagg
     5401 ctgtcggccg catctcgttc ttcgttaact cgggtatgcg ctttgtcgaa gagatgtgta
     5461 aactgcgcgc tttcggcgag atgtgggatg aaatcacgcg tgaccgctat ggcgtgaaga
     5521 ccgagaaatt ccgccgcttc cgctacggcg tacaggtgaa ctctctcggc ctcactgaag
     5581 agcagcccga gaacaacgcc tggcgtatcc tgatcgaagc gctcggtgtg acgatttcac
     5641 agaaggcgcg ctgccgtgcg ctgcagctgc ccgcgtggaa cgaagcgctt tcgctgccac
     5701 gcccgtggga ccagcagtgg tctctgcgcc tgcagcagat actggcatac gaaacggacc
     5761 tgctcgagta tccagacctg ttcgacggct cgccggtgat ccagtcaaaa gtaaactcac
     5821 tcattaccga ggcgaaggcc gagatcgata aagtgctggc gcagggcggt gctgttgaag
     5881 ccatcaagtc gggttacatg aagtcacagc tggtcaagag ccaggctgag cgcatgagcc
     5941 gcatcatgac aggcgagctc aaggtcgtgg gccgcaatat ttatgccgac ggtctgccat
     6001 cgccgcttct cggcggagca gattcgggca tattcaaaat tgagcactct gccgcagatg
     6061 aaaccctcga taacctcaaa aaggtgcgcg agagccgtaa agctgcagaa gcagaagcag
     6121 cgctcaagca gctcgaagac gatgcccgtg gcaatgttaa cctgatgccg gcatccatcc
     6181 gctgcgcgaa ggcgatggtc actacgggcg agtgggcaaa tacactgcgt aaggttttcg
     6241 gcgaataccg cccgctcacc ggcgtcgaag ggcagaaact ttcactgtca gaagatcgtg
     6301 tcgcaacggt ccgcaagaag attgaagcat tcaaagaaaa gcatggccac cgcccgcggt
     6361 tcgtgattgg caaacccggc cttgacggcc attccaatgg ggctgagatg attgccgttg
     6421 ctgcgcggca cgttgggttt gacgttatct attccggtat tcgcctcacg ccagaagaaa
     6481 tcgggcgcgc tgcggtcgaa gagaacgcag acctggtttc aatgtcgatc ttgtctggtt
     6541 ctcacctcga gctggtcgat gcgctgatga agacgctggc acagcacaaa gcagacaagg
     6601 agataccact ggtcgttggc ggtattattc ccgagtcgga ctttgcccgc ctcaaagcga
     6661 tgggtgtgca gcacatcttt actccgaaag actacgatct ggtagcgatc atggaaacca
     6721 accttgagat cgctgaaaag ttcgcctgat cctttacggt actagtcagg ccccaacggc
     6781 ataagcggca gaaaaagctt gccgttggtg gctcattacc gggcaggaaa tgaagagtag
     6841 catgccatct gcgcctatcc aaggtctagc cgaagcctac gttccccggc gattgctggc
     6901 attctacacc cggatggtgc ggttgcttac cccgggcgca tttctcgtac tgctgaccct
     6961 accggttgtt ccggtgctca attactacgg ttaccttttc atttccggct ggcaatcgca
     7021 gccgatgtca cagctctacg gcgcagagct cagtagctgt tggccggtgt tcgtgctcag
     7081 tgctgcggtt tttgtcgttt ctggcggcgg cctcgcacgg ttaggattgc cctcggttct
     7141 gccggtgatc cgccgtctgt cgcgctcagt gcgcctgctc ggtggcgaag cgcctgcaga
     7201 cgaactgcgg cagctgctcc gggatctggt cagagccccg gcggcattta cctggcttgc
     7261 ggtagctgcg tacacgctgc aggaggtttt gttttactgg cagagttatg ctgcttcagg
     7321 tacaacaaac ctgccccttt ttctgaccgt tgacctgttt ctgtacgtga ttcttatcgg
     7381 cctcgtctat tcgattacgg atttcgctct ggcatttctg cgcatgcagg taaagcagca
     7441 tcttgcggct tatggcgaat cttccaatga cctcgtttct ggcggccccg ggtcttaccg
     7501 gctgctttat ctcttttttg tcggtgccgt tgcgaccttt gcgctcgttg ccttcctgca
     7561 gagcaggaac aaccatgtgg tctcagaagc gatccagttt gctgcaatta ccttcatgat
     7621 ctgcggctcg ctggcatttg tttacttttt tacctggagt tattcgctga agcagctgaa
     7681 cgcggcgacc tacagcgtgg cgatgggcgg gcggggccat ttgcccatgc tctccaacga
     7741 ccgcgagatg gttgaacttg cggtaaattt tgacgccgca acttacgaga tcaatacgat
     7801 ccgtaactac ctgaccgaac tcgtcgatga gaagacccac actctttcag aggcttacaa
     7861 cgaactcaag cagctcaagt cgcggcagga cggtgactac tacctgactt caaacctgat
     7921 tgaatcgctg accgaaattc gccccgaaag tgaaacggtc gtcatcgatt ccctggttaa
     7981 gcagttcaag gtctttgaat tcatgggcca cagcaaagaa atcggcggtg acatgaatgc
     8041 gggttacgcc ctgcgcctgc agggtgaaaa gtacacgctg ctgctcaatg ccgacgccat
     8101 gggaaagtca ttgcaggggg cgggcggcgt gctcgtgctg ggatcagctt tgcgttcgct
     8161 gatcgagcgt acgcatatta ccgaagggct gcgcagcctt tcacccgagc gctggctaaa
     8221 gaattcgttc ctcgaactgc accgcctgtt ctccagtttt aatggcagca tgatggcttc
     8281 gatggcctgt ctcctgatcc atgacaattc gggttatacg gtaatggtga atgcggagca
     8341 cccgcatgga atcctgttgc ggggacgtga tgcgcagttc atttctaacc acgagggcct
     8401 gaggaaactt ggcaacaacg cggtcaaagg caggctctgg gtagatacgc tgctgctgca
     8461 cccgggtgat gtcgtcattc ttggttctga cggtcgggat gatatcattg tcggttatga
     8521 tggcgatggc cagccgatta tcaacgatga cgaggaccgc atactgcaga tcgtgaaagc
     8581 gggtgagggg gatttaccgc gtatttacat ggcgctgaaa caatccggtg aaattgttga
     8641 tgacgtttcg ttgttgcgcg tacaatacac gggccggcag tcaaaggtag accaacaggc
     8701 tgtcaccgaa ctctggcgcc agttcggcca ggcaaaagag aatgccggcc gtcttggcat
     8761 tctgcggcgc gtattgcacg aagacgccga caacatgaaa gcctggcggg cgctgctgcg
     8821 tatacacctg cataacaaga acctcatcga tgcggctttt atcgcggagt gcatggtgcg
     8881 cattcgtccc gaacgcgatt ttcttatgta ttcctgcgcg cttttgtact ttcgcaatgg
     8941 aaatctcgag aaggcgctca attttgccga ccgcctgacg ctgcggaact ggcaggtcga
     9001 gcgttacctc gtattactgg ggcgtataca cgaagcgcga caggatgcgg cgggcatgcg
     9061 tgaagcgggg cttctgctca ccgagctcga tggcagccac ccctacggca agaagctgct
     9121 caatttgccc taggccggca gcatcagcaa aaggcgttca agacccagcg caataccact
     9181 ggcactccgc attgcactcg cggtttcaag cagctcttca tctaccggat gttcgggtac
     9241 gccgcgcagc tgcctgattt cgttgtaggc actccagcgc tggcgtatta cattggcgtc
     9301 aaattcttct gaatacgcat tggcgagttc tacgcccgaa atgaaaagtt cagtgcgtgc
     9361 cgcggtcttt ccatcggagc gggcgagcgc cgcaaggcaa tcaggaaaac cttccactgc
     9421 aacgataccc ggcatctcgc gaattacggg ctgtacgact tcgtcaaaca acaggttgaa
     9481 gcaggcgatc tcgccatcga gatgattgaa atgctgtggt accctgccga aacgtgtgcc
     9541 gtacaatcgg gtgtaaggct gaggctggtt gaaatccgga gaaaaactct gctcaacaag
     9601 aacctcgcgc agatcgacat ttgtcacctg cgggggagcg atagtttttc ccacggttgc
     9661 cgcgacgtac cttatgagct ccactgccat ggtgataata tcctgtcgct ggatatcttg
     9721 cacgtaccat tctaccatgg taaattcagg ctggtgtagc ttgcccgcgc ggtcgtcgcg
     9781 aaataccggc gcgatttcgt agattccgcg cgccccgggg ttatgctgca gctcagccgc
     9841 aaaaatcttt ttcatggcaa attcggggga gctcgccaga tagccgacaa aaccgctgat
     9901 gctgctctgc agtgcaaacg tgtcgatgta gggttccatc gctccggcgg aaacgcattt
     9961 gggcgtgcgt acctcgagca ggccgtgctc gttaaagtag ttgcgcgcag cccttatcgc
    10021 ctggttacgc agcagctgcg acgtcatagc tctggcggtt ctttgtgcag cacatcgagt
    10081 gcatgcgcga ggttttcata actctggtaa ccctggcaga ggggaaaata ctgcccgtcg
    10141 cgaccgtaga cgatagtggg aaaagcctcc gcgccgatgt cgcgggcgaa tgtaaactgc
    10201 tgctctgcgc cgggcaggta cctgttttcg gccagctctg agagtgtctg ctttgcatcg
    10261 aggccgtagg ctgtgagaag ccccgcatac tcgtcgtaac gggacatgtc gcggcctttt
    10321 gtgaagttga gcacaagaat atcatgtgcc agctgaaccg tgtctgtacc tggttttgcc
    10381 gcgagcactg cggcaaatac agccgcgggt acccgtgaat cctgctgggt agtgcggtcg
    10441 cgaatgcgcg cgacatacgc ttcgctgaac acagcgccgg tcatctgcgc cacccgcggg
    10501 atggctttct cgatgtatgc gctgaaatct cccagcggac cgatgcgttc acccgttatt
    10561 aatcccccgc tcagggcgct gaagcgaaac cggtcggaga agtctcgtgc caggcgagtc
    10621 accacttcgg atatacccca gcaccagcca cagtagccat cgaagacata aataatttcg
    10681 ggtagggttg cgtgcggtgt accgggcatg ggcaagtgtg cgggggcgag tcagggcgac
    10741 tactaaggga ggggatgtgg gcacaccagg acttgaacct gggaccaacg gattatgagt
    10801 ccgccgctct aa
//
LOCUS       DAOQJF010000076        10718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3178_length_10718_cov_6.231736, whole genome shotgun sequence.
ACCESSION   DAOQJF010000076 DAOQJF010000000
VERSION     DAOQJF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3178_length_10718_cov_6.231736"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLF85_05800"
     CDS             complement(<1..340)
                     /locus_tag="PLF85_05800"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE19209.1"
                     /translation="MGTYPVGERGSYKEEAPAEGGLTPAQVNSILHKLVSLYETKVYP
                     GISFVLMRKGKVLLSRGLGFSHGHGPGDHPQAERREMRPDTPVCLFSTSKAITATVVH
                     TLHEQGALDIN"
     gene            456..929
                     /locus_tag="PLF85_05805"
     CDS             456..929
                     /locus_tag="PLF85_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19210.1"
                     /translation="MRNKLAISALVLWLITLGLGGLLFVRGNRAAQIASDVRIVVDLK
                     PTEREFILAEMRELLRAVHEVHAALATGEKDMAIRAARRVGMGDHDTVVENAQPGMML
                     KIPLEMKKLGHDTHRNFDVIADLLQKNAAEKEIHRELVNLTSKCVSCHSGYRLAR"
     gene            complement(885..3542)
                     /locus_tag="PLF85_05810"
     CDS             complement(885..3542)
                     /locus_tag="PLF85_05810"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19211.1"
                     /translation="MRWKVLWRGVLEWGADPLTGERQRRQHIYTNQGLLYAIALLLIA
                     PLIIETILRLSDWVQNINLYSYRVPVMLHILQYLLAMTLLYALRHWRRWHTLWLALAV
                     LTITSFSSTTTLLLGLNCGFLIFQLGFLPLSFVWLHQRAWLAFLLAIANLILAGITYL
                     YAQSQTPRFPVPEEIAYYLRIGLTLLSAVILLAEMAYLLLITDAGERLRATWLRWSDL
                     GSARYTSTREKQANRIANQVILAFIGLTLAVNIAHLLLTIGVLNIDARRFPMAPIVYM
                     LPAMSLTIILVYIFRIRSRIGHNTAFEALAYATGLTFVFYNSLLQERSLGYQYVLLPL
                     TVAPIFFSEASSLVRLASMVCAPSLFLLREMLPPAGYYPLPEGLGHLMGGFLRAANVF
                     ILSVLVLYLWIKARLAQRFAAIWKSWSAFGTGLFTSPFDKKYRQIQNQLILIFSVVVL
                     LTLAITAVFVIVAVQHGISMVDYSWHYVPVITAQLVLHVVTLWLNRSHKSVIPVATCF
                     ATGIPLIGWVGISLQEEVMVHFFNLALLPLPYIAFARRFSINRILVVFGSLGVFAAIV
                     GAHWFHMHYPPLMPLPIPWMVQFASYVVPAVLLLTFVIYFQYIWNINSQTENETENER
                     RKADELLLNILPENVAYELRESGKTTPILFNSASVMFTDFVGFTKIAEQLSPQELIDE
                     LDKCFSYFDSVCDRYKLEKLKTIGDSFMAAGGIPVPNNTHAIDCCLAAIEIQSFMNQM
                     KEIKAMQEHPYWELRLGINTGNLVAGVVGEKKFAYDVWGDTVNTASRMESSGVIGKIN
                     ISAATYEQVKYLFACRHRGRVKAKNKGEIDMYLLEGIKPKYSVGGAGRVPNDRFREVY
                     EKIARGGRFVPKGPVSPRQSITAVAADTL"
     gene            complement(3539..5557)
                     /locus_tag="PLF85_05815"
     CDS             complement(3539..5557)
                     /locus_tag="PLF85_05815"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19212.1"
                     /translation="MGLLSILQKCTDAGSSFLTSDRARRVNRLQNQFNLLYLSFFLVQ
                     LLASALLGAYLFFFERGNFYPALLLYALPPVASAVFLLQLFRHKLTRPGSPARELIYH
                     GYIVAVVSVMCLLLGNASAFWLFYLPHFVNTFMIFNISRRQAWMFSALTGAFMIPTLL
                     FSARSGSLLPLPRHITYLVSIIIITLFSGLAFALAAATWWRLGLTRRFIRLWVAISNA
                     GTHLFVSEFEKKYRRIQNQIILIFIGLMAFLIATMGVILLDIWLETSEPLLPYILHYG
                     IFIFSEFILLILCLRINSRSAQITGTMIAWFSGLIHIVFLGISLQLSTKFYLFSLVLL
                     PMPYIALSSTQLINRIVAHACSLLLFAAMLGSLWFLKTHQPLRPLPDIITFKIIQYAI
                     PAILVLTYIMYLQYVWKINAETEETLEAEKEKSDRLLLNILPPQVAQELKARGKADAI
                     LFRSATVMFTDFVGFTSIAESMSPEELVSELDRCFSYFDAVCARYRLEKLKTIGDAYM
                     AAGGIPVPNNTHAIDCCLAALEIQAFMNQMKEIKAMQSLPYWELRLGINTGNLVAGVV
                     GEKKFAYDVWGDSVNMASRMESAGMAGAINISRATYELVRFLFRCTYRGRIQAKNKGE
                     VDMYLLEGIRPRFSIDGNGKVPNAEFQRIYERIQAGARLIAAKTLVAS"
     gene            complement(5659..7002)
                     /locus_tag="PLF85_05820"
     CDS             complement(5659..7002)
                     /locus_tag="PLF85_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE19213.1"
                     /translation="MTEAQKKIPGPPGLPVIGNLLDVGTDVLSFLQKVASYGDVAQLG
                     FFNRKITLVSHPDLIQQVLIRDNDRFEKDSGTKMLAKFVLGKGLLTSEGEEHKRMRKN
                     TSPAFNRQRILSYGKIMTDLADQQAAAWQNGATIDIHSEMMAVTSAIVAKALFNMDVS
                     EKVHAIGDALEKIMRIVDVIRMPFSDIAMQLPLPATLEIREGIATLDKIIYETIDEHL
                     ADTTDKGDLLSMMISSRREAGDDMKKARKQLRDEAMTLFLAGHETTANALTWTFYLLS
                     QNPDKYDALCAELDQVLKDRPATIDDIAALPYTRQVVAESMRLYPPAWVIGRTVMHDY
                     EIGGYVIPKGSELWLSQYVVHRDARWYEKPTHFLPERWADGEAEKRPKFSFFPFSAGV
                     RNCIGEQFAWQEAILLLATISRRWRVSLAPGFKVEPLPQVTLRSKNGMSMVLKAR"
     gene            complement(7040..9148)
                     /locus_tag="PLF85_05825"
     CDS             complement(7040..9148)
                     /locus_tag="PLF85_05825"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HNE19214.1"
                     /translation="MRSPFFRQSPFSIFFGALLLPVACASLHPDPVKPADTTVVVHEA
                     RSTLNSLVLELTEANLHRDLPFYPDRRQLYMRLFDGETPLLLYSPLGIETSDADFTHD
                     LSVRNVRLSSAKHDYALAHGKQTRIKKKAELFQVEMQNREGKQLTLEVVMQADAVALR
                     YVLPGNGSTRIVREATGFRFAEGRQAVMQQYQEASKVSPAYEYYFEKVRAGTSDDGKA
                     SIRRIFQPLVGFTGLTIFGSDGWALPALVEAADTKYVLLAEAGLNANHAAVHLTNPAN
                     NLYTVALPALKEGNNRGAVEPTGQLPYATAYRVILCCDLKTIAESTAITDLSAPLDKK
                     FAGKLPQWVQPGKATWDWLSYVSTGDAERQKKYIDAAHEFGWQYTLVDANWNKWNNGN
                     AEPLLRELVDYAGKKRIGLWLWYNSGGPSNTVTEEPRDLMHERSKRRTEFERLRKIGI
                     KGVKIDFWQSDKQAMVQQYIETLEDAADFQLMVNFHGSTTPKGWQRWFPNLMTMEAVK
                     GAEWYQFPVFRGPNAHDNVYYALTRNVIGPMDYTPLVFAQALDQQKISYAHSLALSVV
                     FESGVQHFADNADDAAKGYRKLFANFPFAREFLRNVPVTWDHTLLLEGNPETHVVFAR
                     EKQGVWYIAGISAVSTELQIRQPFTFGTYGNYRAEWITEGETTDSLRYVETELHTGGR
                     QYIDVKVKARGGFVLKLTPK"
     gene            9428..10435
                     /locus_tag="PLF85_05830"
     CDS             9428..10435
                     /locus_tag="PLF85_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19215.1"
                     /translation="MAVKFRPHLLLACLLFLLLAAGALTAQTENATVAGTEVAQSSEI
                     GLATGRVISANIVRRDKKEIVYRLPGSTDEQSLPLGEVIFIRSPQGAYDFIFPDEKPV
                     AAEAAAVPQNAASSQTETLLLVGVGLTLSDNSALTAFAGEYARALAADYNSRLTPAGF
                     VAQPGLEAGSLSALFSAECRWLRGEYMLGLGAGYTVIPKSSAVVSSAAYSGQETINVD
                     GYAVPVTVMVYYRIWGDRSFGFHLGAGGGALFTSVLVTRNFGRGSDYEEARGLAPMLQ
                     LRPELHLRAGPVTLVAAIPVFWAEGRPVGGDISAIQTGSRVAAASFTGAGFNLSVGYN
                     F"
     gene            10528..>10718
                     /locus_tag="PLF85_05835"
     CDS             10528..>10718
                     /locus_tag="PLF85_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19216.1"
                     /translation="MQENGILQLAYPVKLFRGGMRLAIAIGLLGNFTILSALQPETER
                     GEILAAGAEFSRAGSVGSM"
BASE COUNT         2449 a         2816 c         3054 g         2399 t
ORIGIN      
        1 ggttgatatc aagcgcaccc tgctcatgca gcgtatggac aaccgtggct gtgatcgcct
       61 tcgacgtcga gaacaggcag acgggagtat cagggcgcat ctcgcggcgc tcggcctgtg
      121 ggtgatcgcc cgggccgtga ccgtgcgaaa aacccaggcc gcgcgaaagc aggacttttc
      181 ctttgcgcat cagcacgaac gagatgccgg ggtagacctt ggtctcgtac aggctgacca
      241 gcttgtgcag aatactgttc acttgcgcag gcgtgagccc accttcagca ggagcttctt
      301 ctttataact gcctctttcg ccgactggat atgtccccat ggttcagtag gcttcggttt
      361 ttttgcactc acgtaaaccg attaaccttc ggaagcgcac cagccacggc agcgcgaatc
      421 gattgaccgc gcgagctgtg attcgactcg caatcatgcg aaacaaactg gcaatcagcg
      481 ccctggtgct ttggctcatc acgctgggtc tcggcggatt gctgttcgtg cgcggtaatc
      541 gggccgcgca gattgccagc gatgtccgca tcgtcgtcga cctcaagccc accgagcgcg
      601 agttcatcct ggctgagatg cgcgagcttt tgcgagcggt gcatgaagtc cacgcggcgc
      661 tcgcaaccgg cgaaaaagac atggcgatca gggccgcccg ccgcgtgggt atgggcgacc
      721 atgacacggt tgtggaaaat gcgcagcctg ggatgatgct caagataccg ctcgaaatga
      781 agaagcttgg gcatgacacc caccgcaact tcgatgttat tgccgacctg ctacagaaga
      841 acgcagcaga gaaagaaatc caccgtgagc tcgtcaacct gacgtcaaag tgtgtcagct
      901 gccacagcgg ttatagactg gcgcggtgat accgggcctt tgggcacaaa cctgccaccg
      961 cgggcgattt tttcatagac ctcgcgaaac cggtcgttgg ggacgcggcc tgcgccgccc
     1021 accgagtact tcggcttaat tccctcgagc aggtacatgt cgatttcgcc cttgttcttc
     1081 gccttgaccc tgccccggtg acggcatgca aacaggtatt ttacctgttc gtaggttgct
     1141 gcagagatgt tgatcttgcc gatcacacct gacgattcca ttcggctcgc ggtgttcacg
     1201 gtgtcgcccc atacgtcgta ggcgaatttc ttttcgccga cgacgcccgc gaccaggttg
     1261 cccgtattga taccaagtct gagctcccag taggggtgtt cttgcattgc cttgatctct
     1321 ttcatctggt tcatgaaaga ctggatttct attgcggcga gacagcagtc aatcgcgtgc
     1381 gtgttgttgg gcaccgggat acctcctgct gccatgaaag agtcaccgat ggtcttgagt
     1441 ttttccagct tgtaacggtc gcacacggag tcaaaatacg agaagcattt gtccagctcg
     1501 tcgataagct cctggggcga aagttgctca gcgatctttg tgaagccgac gaagtcggta
     1561 aacatcactg aggcggagtt aaaaagaatc ggagtggttt ttcccgattc gcgcaattca
     1621 tacgccacat tttcgggtag aatgttgagt aaaagttcat ctgcctttct gcgttcattt
     1681 tcggtttcgt tttccgtctg cgaattgata ttccagatat actgaaagta gataacgaat
     1741 gtcagcaaga gtacagcagg gacaacgtaa gatgcaaact gcaccatcca gggtatcggc
     1801 aacggcatca atggcgggta gtgcatgtga aaccagtgag ctcctactat tgccgcaaaa
     1861 acgcccagcg agccgaagac aactagtatg cggttaatgc taaacctgcg cgcaaacgca
     1921 atgtatggca gaggcaacag ggccaggttg aagaaatgta ccataacttc ttcttgcagt
     1981 gaaataccta cccagccaat gagcggaatt ccggtagcaa agcaggtcgc caccgggata
     2041 actgatttat gcgaacggtt gagccacagc gtgaccacgt gcagtaccag ctgtgccgta
     2101 attaccggta cgtagtgcca ggagtagtct accatcgaaa tgccatgctg aaccgccacg
     2161 ataacgaata ctgctgtgat agcgagcgtg agcaatacca cgacagagaa aataagaatc
     2221 agctgattct gtatctggcg gtatttcttg tcaaacggtg aggtgaaaag cccggtaccg
     2281 aacgccgacc agcttttcca aatagcggca aaccgttgtg cgagacgcgc ctttatccat
     2341 aggtacaaaa ccagcacaga gagaataaat acgtttgccg cacgcagaaa gccacccatg
     2401 agatgaccaa gaccctcggg tagcggatag tagcctgcag gtggaagcat ctcccgtagt
     2461 aagaaaagtg atggcgcaca gaccatgctg gcgaggcgaa ccagggatga ggcttcagag
     2521 aagaatatgg gtgcgacggt taaaggcaag agtacatatt gatagcccaa agacctttcc
     2581 tgcagaaggc tattatagaa aacaaaggtc aggcctgtcg cataagccag ggcctcgaaa
     2641 gcggtgttat gtccgatacg gctgcggatt cggaagatgt agaccagtat aatggtaagg
     2701 ctcatcgctg gtaacatgta cacgatcggg gccattggga aacgtcgagc atcgatattc
     2761 agtacgccaa ttgtgagcag cagatgggcg atgtttacgg ccagagtgag cccaatgaat
     2821 gcaagaatta cctggtttgc aatacggtta gcttgtttct ccctcgttga agtgtagcgc
     2881 gctgacccga ggtcgctcca gcgtagccag gttgcacgca atctttcgcc tgcgtctgtt
     2941 ataagcagca ggtatgccat ctcagcgagc agtatcacag cgctgagcag ggtcaggccg
     3001 atgcgcaagt aataagcgat ctcttcgggc accggaaacc gcggtgtttg cgattgtgca
     3061 taaagatacg taataccagc tagtatcagg ttggcaatcg ccagcaagaa tgcgagccat
     3121 gcgcgctggt gcaaccagac gaaactcaat ggtaagaagc ccagttgaaa tatgagaaat
     3181 ccgcagttga ggccgagcag aagcgtggtc gtcgatgaaa aagaagtaat agtgagcact
     3241 gctagtgcca gccatagcgt atgccaccgc cgccagtgcc gcagcgcgta gagcaaggtc
     3301 atcgccaaaa ggtactgcag aatatgcagc atgacgggca cccggtagga atagagattg
     3361 atgttctgca cccagtcaga aagcctgaga atcgtttcga taattaatgg tgctatgagc
     3421 aagagcgcta tcgcataaag caagccctgg ttcgtgtaga tatgctggcg gcgctggcgt
     3481 tcgccagtaa ggggatcagc cccccattcg agtacgccgc gccagagtac tttccatctc
     3541 atgatgccac cagggtcttc gcggcgataa gtctcgcgcc ggcctgaata cgttcataaa
     3601 tgcgctggaa ttcggcgttg ggcaccttcc catttccgtc gatcgaaaag cgcggacgaa
     3661 taccctcgag caggtacatg tcgacttcgc ccttgttttt cgcctggatt ctaccgcggt
     3721 acgtgcagcg aaacagaaaa cgcacgagct cataggtggc acgggagatg ttgatcgccc
     3781 ccgccatacc tgcagattcc atgcggctcg ccatgttgac tgaatcgccc cagacatcgt
     3841 aggcgaactt tttctcgccg acaacacccg ccacgaggtt tcctgtattg atgcccagtc
     3901 gcagctccca gtagggaagt gactgcatcg ccttgatttc tttcatctgg ttcatgaaag
     3961 cctgaatctc aagagctgcg aggcagcagt cgatagcgtg tgtgttattc ggtactggaa
     4021 taccgcccgc cgccatgtaa gcgtcgccaa tcgtcttaag cttttcgaga cgataacggg
     4081 cgcagacagc atcaaagtac gaaaagcagc ggtccagttc agagaccagt tcttcgggcg
     4141 acatcgattc ggcaatcgac gtgaaaccga caaagtcggt aaacatcaca gtggcggagc
     4201 gaaagagtat tgcatcggct ttgccacgtg ccttgagctc ttgggcaacc tgcggcggca
     4261 ggatattgag cagaagacga tccgattttt ctttttcagc ttcgagtgtt tcttctgttt
     4321 cagcgttgat cttccagaca tattgcaggt acatgatata ggtcaggacc agtatcgcag
     4381 gaatcgcata ctggatgatt ttgaaagtga tgatatcggg cagcggacgc agaggctggt
     4441 gagtcttcag aaaccaaagc gaaccgagca tcgcggcaaa cagcaagagt gaacaggcat
     4501 gcgctacgat gcgatttatg agttgcgtgc ttgaaagcgc aatatagggc atcggtagca
     4561 gaacgagcga aaataaataa aattttgtgg agagttgtag cgaaatgccc aggaagacga
     4621 tgtggatgag gcccgagaac caggctatca tcgtgccagt gatttgcgct gaccggctgt
     4681 ttatcctgag gcagagaatg agcagaataa attcgctgaa gatgaagatt ccataatgca
     4741 gtatataggg taataatggc tcactcgttt ccagccatat atccagcaat atcactccca
     4801 tagttgcgat cagaaaggcc attaggccaa tgaaaataag aataatctgg ttctgaatac
     4861 gtcggtattt cttttcaaat tccgatacaa acagatgggt gccagcattg gagattgcaa
     4921 cccagagccg gataaatctg cgggtgaggc ccaggcgcca ccaggttgcg gctgccagcg
     4981 cgaatgcgag gcctgagaat agggttatga tgataatcga aaccagataa gtaatgtgcc
     5041 gaggtagagg caagagagaa cccgaacgtg cactgaaaag tagtgtcggg atcatgaagg
     5101 caccggtcag agcgctgaac atccaagcct gccggcggga gatattgaag atcatgaacg
     5161 tgttcacgaa atgcggcagg tagaagagcc aaaaggcaga ggcatttccc agcaatagac
     5221 acatcacgga gacgactgcc acaatgtagc catggtagat caattcacgc gcggggctgc
     5281 cgggcctcgt cagtttgtgt ctgaacagct gcagcagaaa gacagcactg gcgactggag
     5341 gcagagcata aagtagcaag gcaggataga agttgccccg ttcaaagaaa aagagatatg
     5401 cccccagtag ggcgcttgcc agtaactgta ccagaaagaa gctaagatag agcagattga
     5461 actggttctg caggcggtta acgcggcgag ccctgtcgct ggttagaaag ctggagccgg
     5521 cgtcggtaca cttttgcaga atggataaaa gtcccatggg tacagtacgc ggtacactta
     5581 ctattggcgt gcccacagtg caagtgaaac ttaggcgacg ttagatgaga aataccagag
     5641 cacggcagag tccagaattt accgggcttt cagcaccatc gacataccgt ttttcgagcg
     5701 cagcgtcacc tgtggcagcg gttcgacttt gaaacccggt gccagcgata cgcgccagcg
     5761 gcggctaatg gtggccagaa gaaggattgc ctcctgccac gcgaactgct caccgatgca
     5821 gtttctcact ccagcggaga aagggaagaa gctgaacttg gggcgctttt ccgcttcgcc
     5881 gtctgcccag cgttcgggca gaaagtgtgt tggcttttca taccagcgcg catcgcggtg
     5941 cacgacgtac tggctgagcc agagttcgct gcccttggga atcacgtagc cgccgatctc
     6001 gtaatcgtgc atgaccgtgc ggccaatcac ccacgcggga gggtagagcc gcatcgactc
     6061 ggcgacaacc tgccgcgtgt aaggcagtgc cgcgatgtcg tcgatcgtcg ccggccgatc
     6121 tttgagtacc tggtcgagtt cagcgcacag cgcatcgtac ttgtctgggt tttgcgaaag
     6181 caggtagaaa gtccaggtca gtgcgttggc cgtggtttcg tggccggcga gaaagagcgt
     6241 catcgcttca tcgcgcagct gcttgcgcgc ttttttcata tcgtcgccag cttcccttcg
     6301 ggatgaaatc atcatcgaaa gcaaatcacc cttatctgta gtgtcggcaa ggtgttcgtc
     6361 gatggtttcg taaatgattt tgtcgagcgt cgcgataccc tcgcgaatct cgagcgttgc
     6421 cggcagcggc aactgcatcg cgatgtcaga gaacggcatg cggatgacgt cgacgatgcg
     6481 catgatcttt tcgagcgcat cgccgatcgc gtgcaccttt tcgctgacgt ccatattaaa
     6541 gagcgccttg gcgacaatcg cagacgtcac cgccatcatc tcgctgtgta tgtcgatcgt
     6601 ggcaccattt tgccacgcgg cggcctgctg gtcagccaga tcagtcatga tcttgccgta
     6661 cgacagaatg cgctggcggt tgaacgcggg cgaggtgttc ttgcgcatgc gcttgtgctc
     6721 ttcgccttcg ctcgtgagca gtcccttgcc caaaacaaat ttcgcgagca tcttcgtgcc
     6781 agaatctttt tcaaaccggt cgttgtcgcg aatcagtacc tgctgtatga gatcggggtg
     6841 actcacaagc gtgatcttac gattaaaaaa gcccagctgg gcgacgtcgc catagctcgc
     6901 gaccttttgc agaaatgaca gcacatcggt accgacatcg agcaagttgc cgatcacagg
     6961 tagacccggt gggccgggaa tcttcttctg cgcttcggtc atcccttgcc accgatttaa
     7021 atcggtggca agggtcaaat tatttcggcg tcagtttcaa cacaaagccg ccgcgtgctt
     7081 tcaccttcac atcgatgtac tgccgcccgc cggtatggag ttccgtttcg acataacgca
     7141 gcgagtctgt tgtctcgccc tccgttatcc attcggcgcg ataattgcca taggtaccga
     7201 aagtaaaggg ttgccttatc tgtagttctg ttgagacagc actgataccg gcgatgtacc
     7261 aaactccctg tttctcgcgt gcaaaaacaa catgggtttc ggggttgccc tcgagcaaga
     7321 gcgtgtgatc ccaggttacc gggacattgc gcaaaaactc gcgtgcgaaa ggaaagttcg
     7381 caaacagttt gcggtaaccc ttcgccgcgt catcggcgtt gtcggcgaag tgctgcacgc
     7441 ccgattcaaa caccaccgaa agtgccagcg aatgcgcgta actgatcttc tgctggtcga
     7501 gcgcctgcgc gaagacgagt ggcgtgtagt ccatcgggcc gatcacgttg cgtgtcagcg
     7561 cataatacac gttgtcatgc gcgttgggcc cgcgaaaaac gggaaactgg taccactcgg
     7621 cacctttgac cgcttccatc gtcatcaggt tgggaaacca ccgctgccag cccttgggtg
     7681 tcgtgctgcc gtggaagttc accatgagct gaaagtctgc agcgtcttcg agcgtctcga
     7741 tgtactgctg caccatcgcc tgcttgtcgc tctgccagaa atcaatcttc acgcccttga
     7801 tgccgatttt gcgcaggcgc tcgaattctg tgcggcgttt tgaccgctcg tgcataaggt
     7861 cgcgcggttc ttcggttacg gtattgcttg gcccgcccga gttataccac aaccacagac
     7921 caatacgctt cttgccggca tagtcgacca gttcacgcag cagcggttcg gcattgccgt
     7981 tgttccattt attccaattg gcgtcgacca gcgtgtattg ccagccaaac tcgtgcgcgg
     8041 catcgatata tttcttctgc ctttcggcgt caccggtaga gacatagctc aaccagtccc
     8101 aggtggcttt gccgggttga acccactgcg gcaatttgcc agcgaacttc ttgtcgagcg
     8161 gcgcgctgag gtcggtaatc gccgtgcttt ctgcaatcgt cttaaggtcg cagcagagaa
     8221 tcacgcggta ggcagtcgca taaggcaatt gcccggtggg ctcgactgct ccccggttgt
     8281 tgccttcttt aagggcgggg agcgctactg tgtacaggtt atttgccggg ttggtaagat
     8341 gcaccgcggc atggttcgca ttcagacccg cctcggcgag cagcacgtat tttgtgtctg
     8401 cggcttcgac cagtgcgggc agtgcccagc cgtcgctgcc gaagatcgta agaccggtga
     8461 aaccgaccag cggctgaaag atgcggcgta tcgaggcttt gccgtcgtcc gaagtgccgg
     8521 cgcgcacctt ctcaaagtaa tactcgtaag cgggcgagac cttgctcgcc tcctggtact
     8581 gctgcatcac tgcctgcctg ccttcggcaa aacggaaacc ggttgcctcg cgcacgatac
     8641 gtgtcgatcc gttaccgggt agaacataac gcagcgccac ggcgtcggcc tgcataacca
     8701 cctcgagcgt gagctgctta ccctcccgat tctgcatttc aacctggaat agctccgcct
     8761 tcttcttaat tcttgtttgt ttgccgtgcg ccagggcata gtcatgtttg gctgaggata
     8821 gccggacgtt tctgacactc aggtcgtgcg tgaagtctgc gtcgctggtt tcgatgccca
     8881 gcggcgaata gagcagcagc ggggtttcgc cgtcgaaaag tctcatgtag agctgtcgcc
     8941 gatcgggata gaaaggcagg tcgcggtgca ggtttgcttc tgtgagttcg agcacgaggg
     9001 aattcagcgt cgagcgggct tcatggacga ctacagtggt gtccgcaggt ttgaccgggt
     9061 cggggtgaag actcgcgcag gccactggta agagcagtgc tccgaaaaaa atactgaatg
     9121 gagattgtct gaaaaaaggc gagcgcatgc gcaagggtgg gatttagggc gctggtagca
     9181 agccaaagcg agctcggtgt ctccccctcg ccctcaccac cccgcccctc accaggcatg
     9241 gtgaggggca cccctccttg ccaaggaggg gagtagaatt gttctgggac aatggtcgca
     9301 gaagacatat tctctccccc tgctaagggg gagtacggcg tagccgggag ggggttccag
     9361 tctatcgccc atgagttagg agtgattcga attgacagag ccacctttcg ccggacaact
     9421 gacaaccttg gctgtaaaat tccgtccaca tttgctgttg gcttgcctgt tgtttttgct
     9481 gctcgctgcg ggtgcattga ctgcgcagac agagaatgca accgtggcag gtacagaagt
     9541 tgctcagagc tcagaaattg gcctggcaac gggccgggtc atttccgcga atattgtccg
     9601 ccgggataaa aaagaaatcg tctaccgcct gccaggttcg accgacgaac agagtcttcc
     9661 gctcggcgaa gtcattttca tccgcagccc ccaaggcgcg tatgatttca ttttccctga
     9721 cgaaaaacct gtagcagcag aggctgcagc ggtgccgcag aatgcagcat cgtcacaaac
     9781 ggaaacgctt ttgctggtcg gcgtcggcct cacgctcagc gataactccg ccttaaccgc
     9841 atttgccggc gaatacgccc gggcacttgc tgccgactac aactcgcgcc tgacgcccgc
     9901 gggtttcgtc gcgcagccgg gtctcgaagc gggtagcctg agcgcgcttt tttctgccga
     9961 gtgccgttgg ttgcgcggtg aatatatgct cggcctcggt gccggctaca cggtgattcc
    10021 gaaaagcagc gcggttgtca gttcagctgc ctacagtggg caggaaacga ttaacgtcga
    10081 cggctacgcg gtaccggtaa cggtgatggt ctactaccgt atctggggcg accgcagttt
    10141 tggctttcac ctgggcgccg gcggcggagc tctgtttact tcggttctgg tgacgcgcaa
    10201 ttttggccgg ggcagtgatt acgaagaggc tcgtggactc gcacccatgc tgcagctgcg
    10261 gccagagctg cacctgcgcg caggacctgt taccctggtt gccgctattc ccgtcttctg
    10321 ggcagaaggt cggccggttg gcggcgacat ctctgcgata cagacgggca gtcgcgtcgc
    10381 ggcggcgagt tttaccggtg cgggctttaa cctgtcggtt gggtataatt tttaacctgg
    10441 taatccccgg gtagatacgc tcgattttta ttggcgtccc gaatcaaaaa attggcgtaa
    10501 taggattata ttcctgtaat tatacttatg caggaaaatg ggattttgca gcttgcttat
    10561 ccggtaaagc tgtttcgcgg cggcatgcgg ttggctatcg caatcgggct tctgggcaat
    10621 ttcactatcc tgtcagctct gcagcctgaa accgaacgcg gtgagattct ggcagccggt
    10681 gcggagttct cgcgtgctgg ttccgtcggc agcatgca
//
LOCUS       DAOQJF010000077        10678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3200_length_10678_cov_5.026734, whole genome shotgun sequence.
ACCESSION   DAOQJF010000077 DAOQJF010000000
VERSION     DAOQJF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3200_length_10678_cov_5.026734"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..1028
                     /locus_tag="PLF85_05840"
     CDS             375..1028
                     /locus_tag="PLF85_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE19217.1"
                     /translation="MPSEAKFLTERRRERILNALNNRTRRISVLLEDIYDPLNGNAVL
                     RTADGLGIQDIYVAENRHAFNIHSKVSGRASAWLTLHRFRAAGGVQKQDYRIKPEGVQ
                     DATRQAYESLRSSGYTIIATSPRGNSVSLRTLDMMKKYAIALGSEHWGLSDWAIENAD
                     MSVALPMLGFTESYNISVTGAMILHYLSERLRSETSDWRLSDKEKEEIWNEWTKLKR"
     gene            complement(1064..1306)
                     /gene="acpP"
                     /locus_tag="PLF85_05845"
     CDS             complement(1064..1306)
                     /gene="acpP"
                     /locus_tag="PLF85_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE19218.1"
                     /translation="MASTEEIMGKVKKIISEQLGVDEGEVTNESHFIDDLGADSLDTV
                     ELVMALEEEFGIEIPDEDAEKIQTVGDVQKYIEEHS"
     gene            complement(1386..2141)
                     /gene="fabG"
                     /locus_tag="PLF85_05850"
     CDS             complement(1386..2141)
                     /gene="fabG"
                     /locus_tag="PLF85_05850"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNE19219.1"
                     /translation="MKTVLEDLKDAVVIVTGSGRGIGRGIAEKFAEQGSKLVITDIDA
                     ATAEQTAQEIAKMGVETISMACDVSKYDQAEALCNKAAEKWGKIDVLVNNAGITMDGL
                     FLRMKPDQWQKVIDINLTGTYNCAHAAVNHMRKARKGAIVNISSIAAGGNPGQANYSA
                     SKAGVLGFTYALGKELAPMGIRVNAVAPGFIKTPMTDKIPEKLREQMIAAIPLKRPGE
                     PDDIAKVILFLASDLSAYVTAQCVHVNGGLAGL"
     gene            2385..2975
                     /locus_tag="PLF85_05855"
     CDS             2385..2975
                     /locus_tag="PLF85_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNE19220.1"
                     /translation="MQKLYFLSISLPDALEYLILQFKKEIAARYHSVAALKPMRHITL
                     QDTFFYDDTYLPRLQHRLEEVFDCISPFAVELQNFGAFPAHTIFAAIRDFFPFQQLRE
                     SLYWQLKNEITFPPSALGSRKITPHVTIAYRDLTPEAFEEAWSEFCQRELYASFEATS
                     AHLMEHRGKWRSVKEFPFKSKQSPKPDSLQLDLFCA"
     gene            complement(2949..3887)
                     /locus_tag="PLF85_05860"
     CDS             complement(2949..3887)
                     /locus_tag="PLF85_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19221.1"
                     /translation="MRRAFFWIIAVSLLGLWGHHVWLRMGKPVMSDEVRTRFGKRSIQ
                     LSAGKTAFDYAEGEGETVVLVHGFSIPSVVWDNTYKTLARQGVPVLRYDLYGRGFSDR
                     PNVRYDADLFVRQLKELTDEIVGGGKFVLCGLSMGGAIAVHFADRYPERVSRLVLIDP
                     AGYRMTTPVTAKLVQIPGIGDYAGRLMARRAMQKGLAENFAKNVPATIEASALVQTEY
                     AGYADAIVSTLRHMNMSDMEEIYERVGKKKIPTLLVWGKKDKVIPFGNAELVRKAMPH
                     ARFLPLGNAGHIPTSDENEKTHRAILEFLGAEQVEL"
     gene            complement(3936..6659)
                     /locus_tag="PLF85_05865"
     CDS             complement(3936..6659)
                     /locus_tag="PLF85_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19222.1"
                     /translation="MRSANTATRLIDMLKRVLRIFISGIRAKLALFTGSLITLTILIL
                     SWSTVQQQTEILTESYEKQAAISKRYISSLVIELNNIAQNLIQIEEFRQQIAEQKKAL
                     KVYQTQRTVKQDKKIDLGLFKTNLFGALGTKKVRYTLDTFYSRYLNESELKQLESRIR
                     SQISQTTGKPLTAAEWSALQAQARRIVNSEDPAAILSLRAKLDNRIAALLIPSRKKII
                     EETGLEKKLFRIQTFPVTDLSGEQKLAASFDTNLLGKESPLAALEETSAMEANLKQAY
                     ARLIDSPESHVDNDALAFQWNGLEVQTLYSPLYQRPVSTAWARTVVAEPEKILRRKDL
                     VERDRALAARLAEIIPKIKARLKILKEKKPPQPPYTDREFRSFYKDYGAAVSERSFLY
                     DEIFGPEQGDEVADLSAEAVKSLRDSAWEDSILLRYRYDDAEIERFHNSRKERDLARR
                     RWAELRRWVSEARSETAPPNLKRLFAAAQIAKSRSEAEEILWEIDGKPLFADKKPVLA
                     VAILQDNLTAVMRTLVDRTSGVDLVRTNRNKVLLSAVLIGLVSILLAIYISGIVVQKI
                     KRIIRSAEEVGRGNLEVEFEHGGSDEFGNLTVALNQMVSGLNEREKIKGILGSMIDPV
                     VVSEAMKDLQALKRGSEKNITAFFSDVASFSNISEKLTSPQLADLLNEYLSAMTIILK
                     DHEGVLDKYIGDAIVGIFNSPVDVEDHVYKAVIASIEMQKKLTELKAEWTAKKKYIPE
                     ARQMTFRIGLNTGVAKVGFMGTDALASYTMMGDTVNLAARLEAAAKDYGVTILATDAV
                     LEQVKGRIFARRLDVVRVKGKNKPVTLYQIICRKGEETVQLADAVKRYEQGLSAYLQK
                     SWDAAIGFFREAQRVQNVADEACQLLIERCEAYKLNPPPADWDGVYTRTTK"
     gene            complement(6625..7149)
                     /locus_tag="PLF85_05870"
     CDS             complement(6625..7149)
                     /locus_tag="PLF85_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19223.1"
                     /translation="MSTLMTNQEEQQSPVRDDGVFFQKGLPAVVSAIVRNTWAEPYML
                     AHRLFGERAEQALEPFARAVGHTIGILSKPAFAAFRSPDDHPLEDTLKATRIAENATG
                     IYGRDYYVTPKKVVRRIHTCPYRDREGARILCHVGEAAGQEIFAELVPGIRHKVHVTM
                     ARGHAFCEYSYEVD"
     gene            complement(7229..7738)
                     /locus_tag="PLF85_05875"
     CDS             complement(7229..7738)
                     /locus_tag="PLF85_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNE19224.1"
                     /translation="MKSLYLLPLLMFYCASPQPMPTVAKVDLPRFMGKWYVIANIPTF
                     IEKGAHNATEEYSLTPEQEIDTVFTFRADSFDGKEKKYNPRGFVVNRESNAEWKMQFL
                     WPFKSEFLISYLNADYSQTIIGRTDRDYVWIMARTPQISESDYNKLVTFLAANGYDTS
                     KIQKVPQRW"
     gene            complement(7759..8334)
                     /locus_tag="PLF85_05880"
     CDS             complement(7759..8334)
                     /locus_tag="PLF85_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19225.1"
                     /translation="MRETFRRVLAAPLYAIAAIVVLLEDWLWEDLQRFAQYIGRLPVF
                     RQAEALIVALPPWGALAIFALPSLLLIPVKLAALWFISTGHLVMGVGVMVAAKIAGTA
                     LVARLFKLTKPKLLTFNLFARLYGILTRFKARVYEAIRSSWVYRNVRSLAQALRLWWQ
                     EWRATHPSWLGRQWAVLRRHHRGRRDSLPPR"
     gene            complement(8336..8911)
                     /locus_tag="PLF85_05885"
     CDS             complement(8336..8911)
                     /locus_tag="PLF85_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19226.1"
                     /translation="MLAAFDCLVMLSALFAFTKARRAQLTDVLAALGFLLIAGAAGVG
                     TMRYAGLAELKPLHQWASTLASVYGVPAIAAGFFLQAEELRDNKVIGLATVLLIVPGI
                     LFFSSPLYSLIIGICAQLIWILAAVRFQRVYPGIPLRIGLSVAFISVAGLFFTGEGST
                     FSIRNENWFHGLLAAGIVQQGLLFARLKQRG"
     gene            complement(8925..9713)
                     /locus_tag="PLF85_05890"
     CDS             complement(8925..9713)
                     /locus_tag="PLF85_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19227.1"
                     /translation="MRIAAPKSLAAAPLVLLSAADPRAFELVFFTDHDSALSDLAAGK
                     VDLLCTGYTEMARLADPHVHLLLTHCWGLSAIMVPDAAMTDKAVLFAQDDAELVLPFA
                     GSPLDLQVRALLVAAGVSGLKLRNAALPETFAEFQQAKVTAAVFPEPLAALLESGGKG
                     HRLADLSLLWQQTFGELYTPQVSLFTVRETDDQTNFVAALREAVQRCMEPDEQDVTAI
                     AAQLGIPPLVAANALQHVIFALPAAEEARRLEAAYRKTLYSPLR"
     gene            complement(9716..>10678)
                     /locus_tag="PLF85_05895"
     CDS             complement(9716..>10678)
                     /locus_tag="PLF85_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNE19228.1"
                     /translation="DAVFDTNFSADVTIMEEANEFVQRFVQKKGDLPLITSCCPSWTD
                     YMEKLHSDFISNFSSTKSPQQIMGVLAKTYCAQKMKVDPARMKMVSIMPCTAKKYEIT
                     RDHEMFASGFKDVDVVLTTRELARMIRQAGIDFRNLAPETADNFLGAYSGAGTMFGTT
                     GGVMEAALRTAQNVLTGEKMPKLEFEEIRGLAGIKETKVVIAGEEIRIAVAHGLGHVG
                     EVLERVRRAKAEGAPPPYHFIEVMACPGGCIGGGGQPYGVTDEIRRKRMQGLFADDRD
                     RQVRCSHDNPEVQQVYAEFLGEPMSEKAHELLHTTYQARPVYRK"
BASE COUNT         2460 a         2919 c         2773 g         2526 t
ORIGIN      
        1 acagatcaac ttccgtttaa cccatttggg gcgcgccata cgcgccggaa ggccccgctg
       61 catcacccga aggtgccgcg gcggagagtc actttaggaa cactcaacag agcgtcaagc
      121 aaaaagtgtt ttctaggtcg aaagattaag catcgcggcg gcaccctagg cccctgcccg
      181 atgcagtgag aagatgaaaa tcccccaccg acgcggctat tatacgcata atatcccggt
      241 gcgactattt tttgttggga aattatgtct catatgttgc ctcacgcaat ggttaactcg
      301 aaaattgaag attttcagac tatactgccc tattaattgg attgacagtc gctgaaaaat
      361 tgctgccggt ctgtatgccc agtgaagcta aatttcttac agaacggagg cgtgagcgca
      421 tactcaatgc gctgaacaac cgcacccgcc gcatcagtgt tttactcgag gacatctatg
      481 acccactcaa cggcaatgca gtgctgcgta ctgccgatgg gcttgggatc caggatattt
      541 atgttgccga gaaccgccat gcctttaata tacattcgaa ggtttccggc cgcgcttcgg
      601 cgtggctgac gctacatcgg tttcgcgctg ccggaggcgt acagaagcag gattatcgta
      661 tcaaacctga gggagtccag gacgcgacaa ggcaggcgta tgaatcgctg cggagttctg
      721 gttatacaat tattgcgacg agccccaggg gaaactccgt ctcgctgcgc accctggata
      781 tgatgaaaaa atatgcgata gcattgggca gcgaacactg gggactgtca gactgggcca
      841 ttgaaaatgc cgatatgtcg gtcgccctac ccatgctggg gttcaccgaa agttacaaca
      901 tctcggttac cggggcgatg atattgcact atctcagcga gcgcctgcgc tcggaaactt
      961 ctgactggag attgagtgat aaagagaaag aagagatttg gaatgaatgg actaagttaa
     1021 agaggtagcc tgctggccgg ttggccagca ggcaccgaaa acatcaagag tgttcttcga
     1081 tgtacttttg tacgtcgccg acagtctgaa ttttttctgc gtcttcgtca ggaatttcga
     1141 taccgaattc ctcttcgaga gccataacga gctccactgt gtccagtgag tctgcgccga
     1201 ggtcgtcgat gaagtgtgac tcgttggtca cttcgccttc gtcaactccg agttgctcag
     1261 agatgatttt ttttactttg cccatgattt cttctgttga agccatttgg gtctcctttt
     1321 atgccaacag cagcatagct gctaccggca aagttttatt tgccataatt ttcatagtat
     1381 caaaattata ggccggcaag tccgccgttc acgtgcacgc actgcgcggt cacgtaggca
     1441 gagaggtctg acgcaaggaa caggatgact ttggcaatat catctggttc acctggtcgc
     1501 ttgagaggaa tagcagcaat catttgctcc ctgagttttt cgggaatttt gtcggtcatc
     1561 ggggtcttga tgaaccccgg agccaccgca ttgacacgga tgcccatcgg tgcgagctct
     1621 ttaccgagcg cataggtaaa accaagaacg cccgcttttg aggcgctgta gttcgcctgg
     1681 ccagggttac cgcctgcggc gatcgagcta atattgacaa tcgcgccctt gcgcgccttg
     1741 cgcatgtggt tcacggctgc atgggcacag ttgtaggtac ctgtcaggtt gatatcgata
     1801 actttttgcc actggtcggg cttcattcta aggaagagac cgtccatcgt gataccggca
     1861 ttgtttacca gaacgtcgat tttgccccat ttttccgcgg ctttgttgca gagcgcttca
     1921 gcctggtcat atttggatac gtcgcaggcc atagaaatgg tctcaactcc cattttggcg
     1981 atttcctgag ctgtctgctc tgcagttgcg gcgtcgatat cggttataac gagcttcgac
     2041 ccctgctcgg caaatttctc agcaataccg cggccaatac cgcggcccga accggttaca
     2101 atgaccactg cgtctttaag atcttccaaa accgtcttca tagaccataa tttacccgtg
     2161 aatcattgcg gcaacgattt ggcccttaaa atccaatagt atatttcgtc tctcacggaa
     2221 ctattttaat acaccatatg tccctccaaa ttggattttt tagcatgaac atccgtttaa
     2281 cctaaaactg acacttctgc gcatattttt tctgccccat cggctttttt aactttacct
     2341 ctggccggta ccgggttacc cggagcaaac cattgggagg gtatatgcag aaactgtact
     2401 tcttatccat ttcgcttcca gatgcactgg aatatctgat cctgcagttt aagaaagaaa
     2461 tcgcagcgcg ctaccattcc gtcgcagccc tcaagcccat gcggcatatc acgttgcaag
     2521 ataccttctt ctacgatgac acgtacctgc cgcgactgca gcaccggctg gaagaggttt
     2581 tcgactgcat ctcccctttt gcggttgaac tccagaattt cggtgctttt ccggcacaca
     2641 caatttttgc tgcaatcaga gacttctttc ccttccagca actacgcgaa tcgctctact
     2701 ggcagctgaa aaatgaaata acatttccac catctgcatt aggcagcaga aaaattactc
     2761 cccatgtgac gatcgcgtac cgagacctga ctccggaggc attcgaggaa gcatggagcg
     2821 aattctgcca gcgcgagctt tatgccagtt ttgaagcgac ctcggcgcac ctgatggagc
     2881 atcgcggtaa atggcggagc gtgaaagaat ttccctttaa gagcaaacaa tccccaaagc
     2941 cggatagtct acagctcgac ctgttctgcg cctaaaaact ccaggattgc cctgtgcgtt
     3001 ttttcgtttt cgtcgcttgt aggtatatgt ccggcattac ctagcggtaa aaagcgcgca
     3061 tgcggcatag ccttgcggac aagttcggca ttgccgaacg gaattacctt atcctttttt
     3121 ccccagacca ggagcgtcgg gattttcttt ttaccgacac gctcgtagat ttcttccatg
     3181 tctgacatgt tcatgtggcg gagtgttgaa acgatcgcat ctgcatagcc cgcgtattcg
     3241 gtctgcacga gcgcagatgc ttctatcgtt gccggtacat tcttcgcaaa attttccgct
     3301 agccccttct gcattgccct gcgcgccatg agccggcccg cgtagtcgcc gatgcccggg
     3361 atttgcacca gtttcgccgt gaccggtgtg gtcatacgat acccggccgg gtcaatgagc
     3421 acgagccgag agactctttc cgggtaccga tcggcgaaat ggacggcgat agctcccccc
     3481 atcgagagcc cgcagagcac aaatttgccg ccgcccacaa tttcatcggt gagctctttc
     3541 aattgccgga caaaaaggtc tgcatcgtag cgcacattgg gcctgtctga aaatccacgg
     3601 ccatagagat cataacgcag cacgggcaca ccctgccgag cgagagtctt gtacgtgtta
     3661 tcccatacta ccgaagggat ggaaaagccg tgcacaagga ccacggtctc accttcaccc
     3721 tcagcataat caaatgctgt cttgccggcc gatagctgaa tggaacgttt gccgaatcga
     3781 gtgcggacct cgtctgacat cacgggtttt cccatgcgca gccagacatg gtggccccac
     3841 aagcccagca atgatacagc gatgatccag aagaaggcgc ggcgcattgc cgagcgtcag
     3901 aagttcgcac gtgccgtcat ttaagattta gcgccctatt tcgtggtacg cgtgtagacg
     3961 ccatcccagt cggcgggcgg aggattgagc ttgtaggctt cgcagcgttc gatgagcagt
     4021 tggcatgctt catcggctac gttctgcacg cgttgcgcct cccggaaaaa tccgatggct
     4081 gcgtcccagg acttttgcag gtaggcagac aggccctgct cataacgttt taccgcgtct
     4141 gccaattgca ctgtctcttc accttttctg cagatgatct ggtagagcgt tacaggctta
     4201 ttcttacctt tcacacgcac tacatccagg cggcgcgcga aaatacggcc ctttacctgt
     4261 tctaaaactg catcggttgc gagaatcgtc acgccgtagt ctttggctgc ggcttcgagc
     4321 cttgctgcga ggttcactgt atcacccatc atcgtgtagg aagccagcgc atcagtaccc
     4381 atgaaaccga cttttgctac acctgtattg agaccgatac ggaaggtcat ttggcgtgct
     4441 tcgggaatat atttcttttt agccgtccat tcagccttga gttcagtcag cttcttctgc
     4501 atttctatcg aggcaattac cgccttgtag acgtggtcct caacatcaac cggtgaatta
     4561 aaaatgccaa cgatcgcatc accgatatac ttatcgagaa ccccctcgtg atctttaaga
     4621 atgatggtca ttgctgagag gtactcgttc agcaggtcgg caagctgcgg cgaagtgagt
     4681 ttctcggaga tattggagaa agacgcaaca tccgagaaaa aggccgtgat atttttttcg
     4741 ctgccacgct tgagcgcctg caggtctttc atggcttcgc tgaccaccac ggggtctatc
     4801 atggaaccaa gtatgccctt aattttttca cgctcgttga gaccgctcac catctggttg
     4861 agcgctaccg taagattgcc gaattcatcc gaaccaccat gctcaaactc aacttccagg
     4921 ttaccccgcc ctacttcttc tgcactgcgg atgatccgct tgatcttctg caccacgata
     4981 ccggaaatat agatcgccag cagtatggat accaggccaa tgaggactgc cgacaacaga
     5041 accttgttac ggtttgtccg caccaggtcg acgcccgagg tccggtcaac gagggtgcgc
     5101 atgactgcag tcagattatc ctgcaggatc gcgactgcca gcaccggttt tttatcggcg
     5161 aacaacggct taccgtcaat ttcccagaga atctcttctg cctcgctgcg tgatttggca
     5221 atctgtgcag ccgcgaagag ccgtttcaga ttcggtggtg ctgtttcact gcgcgcttct
     5281 gacacccagc gccttagctc ggcccaacgg cggcgcgcaa ggtcacgctc tttacggctg
     5341 ttgtggaagc gttcgatctc tgcgtcgtcg tatcgatatc gcagcagaat agaatcttcc
     5401 caggctgaat cacgcagcga cttcacggct tcggcgctca ggtcagcgac ctcgtcccct
     5461 tgttcgggac caaaaatttc gtcatagagg aaactgcgct cagaaactgc tgcgccgtaa
     5521 tctttgtaaa aactgcggaa ttccctgtcg gtatagggcg gctgtggcgg tttcttttcc
     5581 ttaagtatct tgaggcgcgc cttaatcttc ggaattatct ctgccaggcg ggccgcaagc
     5641 gcacgatcgc gttcaacaag atcctttctg cgcaggattt tctctggttc cgcgacaaca
     5701 gtccgcgccc aggcagtcga aaccgggcgc tggtacagcg gcgaatagag cgtctgcacc
     5761 tcgagcccat tccactggaa ggccagcgca tcgttgtcca catgactttc gggcgaatcg
     5821 atcagccgcg catatgcctg cttcaggttc gcttccatgg cgctggtttc ttcgagagca
     5881 gccagtgggc tttctttgcc cagcaggttc gtatcaaaag aggcagcgag tttttgctcg
     5941 cccgagagat cggtcacagg aaaagtctga atgcgaaaca gtttcttttc aagccccgtc
     6001 tcttcaatga tcttcttgcg cgatggaatg agtaatgcag caatccggtt gtcgagcttt
     6061 gcgcgcagcg acaaaattgc cgcgggatcc tcagaattga cgatgcgccg tgcctgcgcc
     6121 tgcagcgcgc tccattcggc ggcggtcaga ggtttgcccg tcgtctgact gatctggctt
     6181 ctaatacggc tttccagttg cttgagctcg ctctcattca ggtaacgcga gtagaaggta
     6241 tcaagagtat accgtacttt tttggtccca agggcaccga acaggttggt cttgaagagg
     6301 ccgagatcaa ttttcttgtc ctgctttacg gtacgctggg tctggtaaac cttcagtgcc
     6361 tttttctgtt cggcaatttg ctggcggaat tcttcgatct ggatcaggtt ttgtgcgata
     6421 ttattcagtt ctatgaccag tgaagatata taacgtttgg atatcgcggc ctgcttctcg
     6481 tagctttcgg tgagaatttc tgtctgctgt tgcactgtgc tccagctcag gataagaatc
     6541 gtcagggtaa tcaggctgcc cgtgaagagc gcaagttttg ccctgatacc cgagataaag
     6601 atgcgcaaga cacgcttcag catatcaatc aacctcgtag ctgtattcgc agaacgcatg
     6661 cccgcgcgcc atggtcacat gcactttgtg ccgaatgccc ggtaccagct cggcaaaaat
     6721 ttcctggcca gcggcttcgc cgacatgaca gagtatacgc gcaccttcgc ggtcacggta
     6781 aggacacgta tggatgcgcc gtacgacttt tttgggagtt acgtaataat cgcggccgta
     6841 aatgccggta gcattttctg caatgcgcgt ggccttgagt gtatcttcaa gcgggtgatc
     6901 gtcgggagaa cggaatgctg caaaagccgg tttggagaga attccgatgg tgtgcccgac
     6961 cgcgcgcgcg aaaggttcaa gcgcctgctc agcccgttcg ccaaaaagcc ggtgtgccag
     7021 catgtagggt tctgcccagg tattgcgcac aatggcagat acaaccgccg gcaaaccttt
     7081 ttggaaaaaa actccgtcat cgcgcaccgg agactgctgc tcttcctggt tggtcattaa
     7141 cgtagacatg cagctttctc tggccgcaag tcagcttagg acgtaaagtg gtattgcttc
     7201 aggcgttaaa caaacaggag agttagagct accagcgctg gggaactttc tgtattttgc
     7261 tcgtatcgta accgtttgcg gcgagaaacg tgacgagttt attgtagtcg ctttcactga
     7321 tctgcggcgt gcgcgccatg atccagacat aatcacggtc ggtgcgcccg atgatcgtct
     7381 gcgaataatc ggcgtttagg tagctgatga ggaattcact cttgaagggc cagagaaact
     7441 gcattttcca ttctgcattg ctttcacggt taacgacaaa cccgcgcgga ttgtacttct
     7501 tttccttacc gtcaaaactg tccgcacgga aagtgaatac cgtatcgatt tcctgctctg
     7561 gcgtcagcga gtattcttca gtagcgttat gcgctccctt ctcgatgaac gtgggaatat
     7621 tcgcaatcac gtaccattta cccatgaagc gaggtaaatc cactttggca acggtgggca
     7681 tgggctgcgg tgaagcacag tagaacatga gcagtggcaa caagtacagt gatttcatag
     7741 caggaatcta ctgacgattc agcgcggcgg caaactatcg cgcctgccac ggtggtgccg
     7801 cctgagaaca gcccactgcc ttcccaacca cgagggatgc gtggcacgcc attcctgcca
     7861 ccagagccgc aatgcctgcg ccagggaacg gacattccgg tagacccacg aagagcgtat
     7921 tgcctcgtac acgcgtgcct tgaatctggt taagatgccg tataacctgg caaacagatt
     7981 aaacgtaagt aatttcggct tggtaagctt gaagaggcga gccacaaggg ctgtacccgc
     8041 aatcttggcc gctaccatga caccgacacc catgaccagg tgaccggtgg aaatgaacca
     8101 aagcgcagcc agcttcacag gaatcagcag cagcgaaggc agggcaaaaa tcgccagcgc
     8161 cccccagggt ggcaatgcca cgatgagagc ttcagcctgc cgaaagaccg gcaagcggcc
     8221 aatgtactgc gcgaaccgct gcaggtcttc ccacagccag tcttcgagca gcaccacgat
     8281 ggctgcgatg gcgtaaaggg gtgcagcaag aacccggcgg aaagtttcgc gcaagttaac
     8341 cgcgttgttt gagtcgtgcg aataagagac cctgctgcac gataccagcc gcaagcagac
     8401 catgaaacca gttttcattg cggatagaaa acgtactacc ctcaccggta aaaaaaaggc
     8461 cagcgaccga aataaatgcc acgctgagcc cgatacgcag cggaatgccc gggtaaaccc
     8521 tttggaaccg gacagcagca agaatccaga tcagctgcgc acagatgccg atgatgagcg
     8581 agtacaaggg tgaggaaaag aacagtatgc ccgggacgat gaggagcacg gtagccagac
     8641 caatgacttt attgtcgcgc aactcttctg cctgcagaaa aaaacccgca gcaatagccg
     8701 gcacgccgta gaccgaagcc agcgtcgaag cccactggtg caacggcttg agttctgcta
     8761 aaccggcata gcgcattgta ccaacaccgg cggcgccggc gatgagcaaa aaacccagcg
     8821 cggcaagaac atcagtcagc tgtgcacgcc gtgcttttgt aaaggcaaag agcgccgaaa
     8881 gcataacgag acaatcaaat gcagcaagca tgagatgtgc tggcttagcg cagtggggag
     8941 tacagtgttt tccggtaggc cgcttcgagc ctgcgggctt cttccgctgc cggcagcgca
     9001 aaaatgacgt gctgtagtgc attcgccgcc accagcggcg gtatgccgag ttgagctgct
     9061 atcgctgtaa cgtcctgctc atcaggttcc atacagcgct gcactgcctc tctaagggcc
     9121 gcgacgaagt tcgtctggtc gtcagtctcg cgcacggtaa atagcgaaac ctgcggcgtg
     9181 tacaactcgc cgaaagtttg ctgccagagc aacgacaggt ctgcgagcct gtgcccctta
     9241 ccgccggatt ccagcagcgc agcgagcggc tcggggaaaa ccgccgcggt gacctttgct
     9301 tgctggaact ctgcgaatgt ttcgggcaac gctgcgttgc ggagctttaa cccggaaacc
     9361 cctgcagcaa ccagcagagc cctcacctgc aggtcgagcg ggcttccggc aaagggcaga
     9421 acgagttcag catcgtcctg cgcgaaaaga acggctttgt cagtcatggc ggcgtcgggt
     9481 accatgatcg ctgaaaggcc ccaacaatgg gtcagcaaca aatggacatg cggatcagcg
     9541 aggcgcgcca tttctgtata gccggtacac aacagatcaa ccttgccagc tgcaagatct
     9601 gacagtgcag aatcatgatc agtaaaaaaa acaagctcga acgcacgtgg gtctgcagcc
     9661 gacagcaaca cgagcggggc agcagcgaga gatttaggtg cagcaatacg caaggctatt
     9721 tccggtaaac gggtcttgcc tggtacgtcg tgtgcagcag ttcgtgcgct ttttctgaca
     9781 tgggctctcc caggaattcc gcgtaaacct gctgcacctc agggttgtcg tgtgaacagc
     9841 gcacctgccg gtcacggtcg tcggcaaaca gaccctgcat acgtttcctg cgaatttcgt
     9901 ctgtcacacc gtacggttgt ccgccacctc cgatacagcc gccggggcag gccatcacct
     9961 cgatgaagtg gtaaggcggc ggagctccct ctgctttggc gcggcgcaca cgctcaagca
    10021 cttcgccgac atggccgagg ccgtgcgcga cagcaatgcg aatttcttca cccgcgatca
    10081 caacttttgt ttcctttata ccagcaagtc ccctgatctc ttcgaattcg agcttcggca
    10141 tcttctcgcc tgtcaggaca ttctgtgctg tacgcaacgc tgcttccatc acaccacctg
    10201 tggtaccgaa cattgtcccg gcacctgaat aggcgcccag gaaattatcc gcagtttccg
    10261 gcgccagatt gcggaaatcg attccggcct gacgtatcat gcgcgcgagc tcgcgcgtcg
    10321 tgagcaccac atcgacgtcc ttaaagcccg aagcgaacat ttcatggtcg cgcgtgatct
    10381 cgtatttctt ggcagtacac ggcatgatag aaaccatctt catgcgtgcc ggatcaactt
    10441 tcatcttctg tgcacaatac gtctttgcca gcacacccat aatctgttgc ggcgacttgg
    10501 tcgaggaaaa attcgaaata aaatcgctgt gcagtttctc catgtaatcg gtccacgaag
    10561 ggcagcacga ggttatgagt ggtaaatcgc ccttcttctg cacgaagcgc tgaacaaatt
    10621 cgttcgcctc ttccatgatc gtgacgtccg cggaaaagtt ggtatcaaag accgcatc
//
LOCUS       DAOQJF010000078        10493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3286_length_10493_cov_5.013604, whole genome shotgun sequence.
ACCESSION   DAOQJF010000078 DAOQJF010000000
VERSION     DAOQJF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3286_length_10493_cov_5.013604"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..806)
                     /locus_tag="PLF85_05900"
     CDS             complement(90..806)
                     /locus_tag="PLF85_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19229.1"
                     /translation="MTQNPQNSIGKITVIHGPMFSGKTEELLRRVRRAKIARKRVQLF
                     KPAIDNRYHATHVLPHFLAHAGDESVQVGEAAHVVAHARDILPLVEKDTDIVAIEEAQ
                     FLDDFLFEVIRRLSRQGKDLMLSLLDQDYRRLPFPIPGSERSVGDYLAIAHESLKLAA
                     ICVSCGNDAHHSQKLTLKSIAADGQPVYVPASFYSEVVTVGTSQEIAQEKLFADKLRD
                     FPENIYEARCRNCHVVADEP"
     gene            complement(803..1591)
                     /gene="proB"
                     /locus_tag="PLF85_05905"
     CDS             complement(803..1591)
                     /gene="proB"
                     /locus_tag="PLF85_05905"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNE19230.1"
                     /translation="MNKTLEARIKNATHVVLKFGTGVLTEHIHAGSTKYFRQIAKECL
                     WLQSQGKKVIIVSSGAGGFGRVILKKSRNIVLPNATLKEKQSLASLGQSLLIDTYRTA
                     FAKENLPAAQILLTRTDFENKRHLQNLRATLSQLLDWGAVPVINENDAIANEEIKVGD
                     NDNLSADIALLYPKSLLVLLTTIDGFYRNKERVPHIARVSADIKKHAGNAAEGGTGGM
                     ITKLQAAEKILRADQAMIIAAGKDIRIVRRILEAKDAGTWVYNA"
     gene            complement(1597..2703)
                     /gene="dxr"
                     /locus_tag="PLF85_05910"
     CDS             complement(1597..2703)
                     /gene="dxr"
                     /locus_tag="PLF85_05910"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNE19231.1"
                     /translation="MKRRFLLFGATGSIGDSTLDVLRQRGDDFELVGFTWHNNEEKAR
                     ALQAEFPRAVAFSTALADADTQTRRLLDLRHDFVIVAIVGAAGVNATLEAARRGDTIL
                     LANKESLVIAGDVVMAQVKASGAKLIPVDSEHSSLYRLLQGAAAQHPAKVYLTASGGP
                     LLHVHGDAFFNASKEVVLKHPTWVMGQKITVDSAGLTNKALEIIEAHYLFGLPYEQVQ
                     AVIHPQSYVHAMVELSDGTVLQHISQPDMRYPIAWAMYYPQPSPDTVQGRVPASYPTL
                     EFFPVDTAKFRSYTLGRSAGEKGKYYPAVFNAANEAAVASFLAGNIRFGEIATLIAEA
                     LEASYPAYDYTSIDGLLQYDNAARETVQKRVFST"
     gene            complement(2732..3649)
                     /locus_tag="PLF85_05915"
     CDS             complement(2732..3649)
                     /locus_tag="PLF85_05915"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNE19232.1"
                     /translation="MGETPKRLISGLILAGIFIFTITWQQMYMFPLYLFVLTFAVLGI
                     VEFYQLAEAKIQAKVPKTLGIISTVLLLTIVYLGWQSTYYVPGQPLFSPEVKRFLNLL
                     KFNYALLGGTIFVFVFSLFKIQLLKNKVENSLFILAIYTLSVIYIPFTFSHLFLLYSL
                     ENGLFYIWLVAWATAMSDAAGYFMGRTFGRNKVGFAVSPNKTYEGYILGCFLQMGLVA
                     GFYAVAKANFNVPQYSYVEITVFGFVVYLASILGDLTESLIKRDAGIKDSGSLIPGHG
                     GVLDLLDAMLITIPAAYYYFYIVQELRSI"
     gene            3737..6277
                     /locus_tag="PLF85_05920"
     CDS             3737..6277
                     /locus_tag="PLF85_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNE19233.1"
                     /translation="MRNFSLFITLLFLSACNSIINETERVRKSYFYQNSESPTVTATD
                     PADGAIGPNTQTYIDITFSAPVDLATVTVQSSFGACSGSLQISYDGFGNCLAGTIDGS
                     ANPRIRFIPTVFPKGLGLQIRASGAVTNPYGRPIEAYTSPTGFRLAAPCGGQNCFFSY
                     STPLIANAGTASWIFAVRSGPHSGKYLAYSSSGTTTTLIDPLTVSSQSGKALCFTPNF
                     GTYSFYVSTGALAGKEIIVAGGSSNATCIYDHATDTMSTGNTLAIAAGYGGFPLLPQS
                     GTELGNVLMLQGGGLTGINRFNIAAGTFANANPLASTGGIAMQAHSIRLQTGTNIGQH
                     LVFHGVASATTTWFNETTGTMTSGPAVSTLNTGSGSFEVTSGTRSGQVITVLGGNTTS
                     TYVFNTTSLTGAAGGPAVSGNVTSGGRLLRQASTNSYDAPLYLHGAGFQSSIYNSATG
                     NFEAGPLTTGAILDGSAAAFIPSTANGGAFLIVNGSSGGVQTPSTSVYFPQTNSFSGT
                     RMPTNVPNPGSNSFVISGGPQNGRTLIVSGALSRETAVFDPLRFEMQRGPQLASPTTA
                     NALNYRLGSGRAVVFHGNAATYEIYDPASHSFSTSGGPSLSQPLGQAAVAFGLPGTSK
                     FVVMHGGSATSGDMFDETTLAAPTGVTSPCAVGGVAFTLRYTNTAVSQQRQLVFCSGN
                     TMGRFDHATGTFLTSLTLAGAAGVGIQGFVISGGTHNGKVLIIHGGGSTTTSLIDTTV
                     NPETVSLGPSPSPACSTMPNAGAQIMPVTSGTSNGRVVLLPGAGSSASCIYDPTAHSF
                     SSGNAVGNGVGFQLTAGALAFRTNGGLYPTSFIVLSGGNKNVWSAYVP"
     gene            6274..7401
                     /locus_tag="PLF85_05925"
     CDS             6274..7401
                     /locus_tag="PLF85_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19234.1"
                     /translation="MKSITFYFQQRFSPLLVTLFITALLVRIALLFVPGFSTDPQRFL
                     SDGINLLEGRNPYTAAPALPITYGHLRSFYTPLMEGFFAFGAWLMHDPFVFRLLGGAA
                     ELLFLGWFFVRRRSRPLPLLLTLFLLFNPVSLHEIWREGHLDHIGAFFLYFAVVSGRS
                     AFAHRRGKLRGLLFLLLSIAWKFTGIIALAFRLRKLGSSPVQRTVLRLADSFVWMGIA
                     AFALQLLPVYLFTQFAERGLTVYTTYWHHGNGIVHLLHGLGFADAHGIYLVQRLILLA
                     LLLIGGLYLTGRLSYTNTLCLGLGTLLVLFPVQHPWYYFMLFPFILLYRRWRKPFMVL
                     CCLAPLSYLGYTASLKSTGFWIMLVFWLFVIIRLPARQPGA"
     gene            7469..8368
                     /locus_tag="PLF85_05930"
     CDS             7469..8368
                     /locus_tag="PLF85_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNE19235.1"
                     /translation="MPLLLRSILLLCLLAGGLWPEQANQPRYFFYKATRNDATFYLLG
                     SMHMGKPADPEYPEKIYQALRKSSMLILEGEVRREKMRQPDMTLTYLPAKQKITDLLS
                     THERSQLKKMTDTLGVPMGQFEHFQPWFIEFMFGYRLAYNQGYVLEYGTEHRLLRYVE
                     RQLPAKERPRIFTLERFDEVIVNMSRLPLADQMKRFRAFLSYSDRVGESNALEMADHW
                     RNGDDEKVLEIFHYYNKQNASTSDAYAKVLLFDRNRRMAERLDLVARYPGQYFVISGA
                     LHLIGEKNILEYLRAAGFTVERL"
     gene            8365..9273
                     /locus_tag="PLF85_05935"
     CDS             8365..9273
                     /locus_tag="PLF85_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE19236.1"
                     /translation="MSRKWGCEKCDGLGYRTQPQLTRTRATQILALCDCVEALCRVCP
                     GDQVSPYVYLDEKSGRILPCDCKGARDSYSRVKDLFSHSNIPLKYRMHRLREFNLQPQ
                     YPNPTKSPELENARRQATIALTAAFDRARLFIEAQKKLLADPKQVTPADLKGLLLIGP
                     PGTGKSLLAAMILNELIITARMSCRYIKISRDFFQQLRATFSNDSGTYGRTDSVFNEI
                     ARQDVLVIDDFGIQSDSEWEQRTLYDLIDARYENDLPTIITSNIDLDAVRNLFKGRIY
                     SRFTEMLHTVEFFQVRDYRQDIHSNT"
     gene            9344..>10493
                     /locus_tag="PLF85_05940"
     CDS             9344..>10493
                     /locus_tag="PLF85_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE19237.1"
                     /translation="MSIDKSLLDFNLSDELVEIGELVREICEKEVVPRRAELDEKEAY
                     PFEIFAKFKEAGLPGIMYDAEYGGMGVGLFAAIVFSEIVSEYCLGVNTAISATKLGAL
                     PIEHGGTEEQKKKYLSAFASGEKIGAFGLTEPNAGSDVPSLSTTAVKKGDRYVLNGVK
                     QWISNAGVADVYTIFALTDKSKGPRGMSCFIVEKDTKGLSFGKLENKMGIRCSHTRQV
                     ILEDVEVPEENLVGLKPNRGFIHAFQTLSSSRPAVAASAVGLAVGAYKEAVKYARQRE
                     QFGKNIINFQAVQHMLADMATRIEQARLLTYRAAKYAMAGHPEAAKFSAMAKYAASET
                     AVAVANDALQLHGGYGFTKDYPVEKMYRDAKILTIYEGTSQIQKNEIGA"
BASE COUNT         2182 a         2944 c         2962 g         2405 t
ORIGIN      
        1 ggaaattttt cagcgttggc gcgaaaagct gcgtatacgg tgggaaaatg ctgcattggc
       61 gctctgccag ggaaactgac gcggtggcgt cacggttcgt cggcgacgac atggcaattg
      121 cggcagcgtg cctcgtaaat gttttccgga aaatcgcgca gcttgtcggc aaagagtttc
      181 tcctgcgcaa tctcttgcga agtgccgacg gtgacgacct cgctgtagaa cgaagcgggt
      241 acgtagacag gttgcccgtc ggcggcgata cttttcagag tcagcttctg gctgtggtgt
      301 gcatcgttgc cgcaggatac gcagattgcc gcgagcttca acgattcgtg cgcaatggcg
      361 aggtaatcgc ccacgctgcg ctcagaaccc ggaatcggga acggtagtcg gcggtaatcc
      421 tggtcaagca gagagagcat gaggtctttg ccctggcgcg acaacctgcg gatcacctcg
      481 aacaggaagt cgtcgagaaa ctgcgcttct tcgattgcaa caatgtctgt gtccttttcg
      541 accagcggca gaatatcgcg tgcatgcgcc acgacatgcg ccgcctcgcc gacctgcacg
      601 ctttcgtcgc cggcgtgagc aagaaaatgc ggtaatacat gtgtcgcgtg gtacctgttg
      661 tcgatcgcgg gtttgaaaag ttgtacgcgt ttacgcgcga tcttagcgcg ccgcacgcgg
      721 cgcagtagct cttcggtctt gcccgagaac atcgggccgt gtatcacggt gatcttgccg
      781 atggagttct gcgggttctg tgtcatgcgt tgtaaaccca ggtgccggcg tcttttgcct
      841 cgagtatcct gcgcacaatg cggatatctt tgcccgcagc aataatcatc gcctggtcgg
      901 cgcgcaatat cttttcagca gcctgcagct tggtgatcat gccgcctgtt ccaccttcag
      961 cggcgttgcc tgcgtgtttt ttgatgtcag cgctgacgcg cgcgatatgt ggcacgcgct
     1021 ctttgtttcg gtaaaagcca tcgatggtgg tcagcagcac cagcagcgac ttggggtaga
     1081 gcagcgcaat gtcggccgac aggttgtcgt tgtcgcccac cttgatctct tcgttggcga
     1141 ttgcatcatt ttcattgatc acaggcacag cgccccagtc gagcagctgg ctgagtgtag
     1201 ctctcaggtt ctgcaggtgg cgtttgttct caaaatcggt gcgcgtgagc agaatctgcg
     1261 ccgcgggcag attctctttg gcgaatgccg tgcggtaggt gtcgatgagc aggctttgcc
     1321 ccagtgaagc gagggattgt ttttccttca gcgtcgcatt gggtaatacg atattgcggc
     1381 tcttcttcag aataacccgg ccaaaaccac cggcaccgct cgagacgatg atgacctttt
     1441 tcccctggct ttgcagccag aggcattctt tcgcgatttg cctgaagtat ttcgtgctgc
     1501 cggcatggat gtgttcggta agtacgccgg tgccgaactt cagcaccacg tgcgtggcgt
     1561 tcttgattct ggcttcgagg gtcttgttca tagatctcag gtggaaaata cccgcttctg
     1621 cacggtttcc cgcgccgcgt tgtcatactg caacaggcca tcaatcgaag tataatcgta
     1681 agcggggtaa ctggcctcaa gcgcttcggc aatgagcgtg gcaatttcac cgaagcggat
     1741 attgcctgcg agaaaggagg ctaccgctgc ttcgttggcg gcattgaata cggccgggta
     1801 gtatttgccc ttttcgccgg cactgcgccc gagggtgtat gaccggaatt tcgcagtgtc
     1861 gacgggaaaa aattcgagcg ttgggtagga agctggtacg cggccctgta cggtgtcagg
     1921 cgaaggctgc gggtagtaca tcgcccaggc gatcgggtag cgcatgtcgg gttggctgat
     1981 gtgctgcagc acggtgccat cgctgagctc gaccattgcg tgtacgtaac tctgcgggtg
     2041 aatgaccgcc tgcacctgtt catacggcag gccaaaaaga tagtgcgctt cgataatttc
     2101 gagcgccttg ttggttaaac ctgcagaatc tacggtaatc ttctgcccca tgacccatgt
     2161 tgggtgcttc agcaccactt ctttcgatgc gttaaaaaac gcgtcgccat gtacgtgcaa
     2221 cagggggccg ccgctggcag ttaagtacac ttttgcaggg tgctgcgcag cagcaccctg
     2281 caaaagcctg tagaggctcg agtgctctga atcgacggga ataagttttg cgcccgatgc
     2341 cttcacctgc gccatcacga cgtcgccggc gatcacgagg ctttctttgt tagccagcag
     2401 tatcgtgtcg ccgcgccgcg cggcttcgag cgtggcgttg acgccggcgg cgccgacaat
     2461 cgcgacgatg acaaagtcat gccgcaggtc gagtagccgg cgcgtctggg tgtcggcatc
     2521 ggccagagca gtggagaaag ctacagcacg gggaaactcc gcctggagcg cacgcgcctt
     2581 ttcttcgttg ttatgccagg taaaaccgac gagttcgaag tcgtcgccgc gctggcgcaa
     2641 cacatcgagc gtcgaatcgc cgattgaacc tgtcgcgccg aaaagtagga acctgcgctt
     2701 catccgttgc gcagcgctgt cccctcagcc attagatcga cctgagttct tgcacgatat
     2761 agaaatagta gtaagctgct ggtatcgtga tcagcatggc atcgagcagg tcgagtacgc
     2821 cgccgtgccc gggaatcagc gagccggaat ctttgatgcc cgcatcgcgc ttaatcagcg
     2881 attcggtcag gtcacccagg atagaggcga gatacacaac gaagccaaaa accgtgattt
     2941 ctacgtagct gtactgcggt acattgaagt tggctttcgc gacggcgtag aaacctgcaa
     3001 cgaggcccat ctgcaaaaag cagccgagaa tataaccttc gtacgtcttg ttggggctga
     3061 ccgcaaaacc gaccttgttg cggccgaagg tgcgacccat aaaataaccc gccgcgtcag
     3121 acatggccgt cgcccaggca accagccaga tatagaaaag gccgttctca aggctgtaga
     3181 gcaaaaagag gtgcgaaaaa gtgaagggta tatagattac cgacagcgtg tagattgcca
     3241 gtataaagag ggagttttca accttgttct tcagcagctg gatcttaaag agcgaaaata
     3301 caaaaacaaa aatcgtaccg ccgagcaggg catagttgaa cttgagcagg ttcagaaacc
     3361 gcttcacttc gggtgaaaac aggggttggc ccggtacgta gtaggtcgat tgccagccca
     3421 ggtaaacaat ggtcagcagc agtaccgtgc tgatgatacc cagcgttttg ggtactttcg
     3481 cctgaatttt ggcttctgcc agctggtaga actcaacgat acccaaaacg gcaaaggtca
     3541 ggacaaagag gtacagcggg aacatgtaca tctgctgcca ggtaatggta aaaatgaaaa
     3601 tacccgccag aatcagccca ctgatcaggc gcttcggagt ctcacccatg gtagggtgac
     3661 cgcatttttt tcttatcagg ctgtcaacga tagccatggt tggtgcactg cgggcagtgc
     3721 ctgtagggta ccaggtatgc gcaacttttc cctctttata actctgctgt ttctttcagc
     3781 ctgtaattcg ataatcaatg agacggagcg cgtacgcaag tcgtactttt accagaactc
     3841 agaatctccc actgttaccg cgaccgaccc cgccgatggc gcgattggcc ccaacacaca
     3901 gacctacatc gatattacct tctctgcccc ggtagacctg gcgacggtta ctgtccagtc
     3961 gtcttttggc gcctgctcgg gttcgctgca gatcagttat gacggttttg gcaactgcct
     4021 cgcgggcacg atcgacgggt cggcgaatcc gcgcatccgt tttataccaa cggtgtttcc
     4081 caaagggctg ggcctgcaga ttcgggcctc tggagctgtg acgaatccgt atggcagacc
     4141 cattgaagcc tacacctcgc cgaccggctt caggctagcc gcgccgtgcg ggggacagaa
     4201 ttgcttcttt agctactcca cgccgctcat agccaacgcg ggcactgcct catggatttt
     4261 tgcggttcgc agtggcccgc actcgggcaa gtacctggcc tattcttcga gcggcacaac
     4321 cacgacgctg attgatccgt tgacagtctc ttcacagagc ggtaaggcgc tgtgttttac
     4381 accgaacttt ggtacttata gtttttacgt ttccaccggc gcacttgcgg gcaaagagat
     4441 tatcgttgca ggcggtagct cgaacgcgac ttgcatctac gatcacgcca ccgacacgat
     4501 gagcacaggc aataccctgg ctattgctgc aggttacggt ggatttcccc tcttgcccca
     4561 gtctggcacc gagctgggta acgtgctcat gctgcagggt ggtggactta ctggcataaa
     4621 ccggttcaat atcgccgcag gtacatttgc caatgcaaac ccgctcgcca gcaccggcgg
     4681 catcgccatg caggcgcact caattcgcct gcaaactggt acgaatatcg gccagcacct
     4741 ggtttttcac ggggtggctt ctgccacaac cacctggttc aatgaaacga cgggaacgat
     4801 gaccagcggg cctgcggtat cgacactcaa taccggttcg ggaagtttcg aggtgacaag
     4861 tggcacgcgc agcggccagg ttatcacggt gctcggaggc aacaccacca gtacgtatgt
     4921 cttcaacacc acgtcgctca ccggtgctgc cggcggcccg gcggtttctg gcaacgtcac
     4981 cagcggtggc cgcctgctga ggcaggccag caccaattca tatgacgctc ccctctacct
     5041 gcacggcgcc ggattccagt cgagtatcta caattctgcg accgggaatt ttgaggcagg
     5101 gcctttgacg acgggcgcta ttctcgacgg ttcagcggct gcgtttattc cttcaacggc
     5161 aaacggcggg gcgttcctga ttgtcaacgg cagttctggc ggtgtccaga ctccttcgac
     5221 ctcggtctat tttccgcaga ccaattcatt cagcggcacg cgcatgccga cgaatgtacc
     5281 gaatcccggc tccaactctt ttgttatctc tggcggtcca cagaatggca ggaccctgat
     5341 tgtttccggc gcgctcagcc gcgagaccgc ggtgtttgat ccgctgcggt ttgagatgca
     5401 gcgcgggccg caacttgctt cacccactac cgctaacgca ctgaactacc gcctgggttc
     5461 gggcagggcc gtggtgtttc atggcaatgc cgcgacctat gaaatctacg atcctgcttc
     5521 tcacagtttc agtacttccg gcggcccgtc gctgagccag ccgctcgggc aggcagctgt
     5581 tgcgtttggc ctgcccggaa cctcgaagtt cgtcgtgatg cacggcggtt cagcgacttc
     5641 gggcgatatg tttgatgaga caacgcttgc agcgcctact ggtgttactt cgccgtgtgc
     5701 ggtaggtggt gtggcattca cgctccgtta taccaacacc gccgtttcgc agcagcgcca
     5761 gctcgtcttc tgttccggaa atactatggg caggtttgat cacgctacgg gtacctttct
     5821 cacttcgctc acgcttgccg gtgcggcagg tgtcggtata caaggtttcg ttatttcagg
     5881 cggaacgcac aatggcaagg tgctcatcat ccacggtggt ggctcaacga ccacgagcct
     5941 gatcgatacg accgttaacc cggagacagt atctttgggc ccttcgccgt cgccggcctg
     6001 ttcaacgatg ccgaacgcgg gggcgcaaat tatgcctgtt accagcggca ccagcaacgg
     6061 cagggtggta ctgttacccg gtgcaggatc atcggcatca tgcatctacg atccgactgc
     6121 acacagtttt tccagcggca atgcagtggg caacggggtc ggttttcagc tgacagcggg
     6181 cgcgctggcc ttcaggacaa atggcggcct ttatcccaca tcattcattg tactttcagg
     6241 cggtaacaaa aacgtctgga gtgcatacgt cccgtgaaga gtattacgtt ctactttcag
     6301 caacggtttt ctccgttgct ggtgaccctg ttcatcaccg ctctactcgt gcgcatagcg
     6361 ctcttgtttg tgccgggttt ctcaactgat ccgcagcgct tcttgtctga cggtatcaac
     6421 ctgctcgagg gccgtaaccc gtatacagcg gcacccgcgc tgccgattac ctatggccac
     6481 ctgcgttctt tctacacccc gctcatggaa ggttttttcg ctttcggcgc ctggctgatg
     6541 catgacccct tcgtgtttcg cctgctcggt ggcgcggcgg agcttctctt tctcggctgg
     6601 tttttcgtgc gcaggcgcag ccggccactc ccgctcttgt tgacgctgtt cctgctcttt
     6661 aacccggtca gtctgcacga gatctggcgc gagggacacc tcgatcatat tggcgcattc
     6721 ttcctttact ttgcggtagt gagcggtcgc tcagctttcg cgcaccggcg cggcaaattg
     6781 cgcgggctgc ttttcctgct gctcagtatt gcatggaaat ttacgggtat tattgccctt
     6841 gcgttcaggc tccggaagct ggggagctcc cccgtgcagc gtacagtgtt gcgacttgcc
     6901 gacagcttcg tctggatggg gatagcggct ttcgcgctgc agctcttgcc ggtatacctc
     6961 ttcacacaat tcgccgagcg cggcctgacc gtctatacga cttactggca ccacggcaac
     7021 ggtattgtgc acctgctgca cggcctggga tttgccgacg cgcacggcat ctatctggtg
     7081 cagcggctga ttttgctggc gctgctgctg atcggtggcc tgtacctcac ggggcggctg
     7141 agttacacga atacgctctg cctcggcctc ggcacgttgc tagtgctttt tcctgtgcag
     7201 cacccgtggt actactttat gcttttcccc tttatactac tctaccgtcg ttggcgtaag
     7261 ccgttcatgg tgctctgctg cctggcgccg ctgagttacc tcggctacac cgcgagcctc
     7321 aaatcgacgg ggttctggat tatgctggtg ttctggctat tcgtcatcat tcgactgcct
     7381 gcgcggcaac ctggcgcgta atcattgcca gctgccgggc ccgaatcact ttaccgggca
     7441 gggcaaattg ccgcaaggga aattacgcat gccgctactg ttacgctcca ttttgctgct
     7501 ctgtttactt gctggtggcc tgtggcctga gcaggctaac cagcctcgct actttttcta
     7561 caaggcaacg cgcaacgatg cgacgttcta tctgttgggt tcgatgcaca tgggtaaacc
     7621 tgcagacccg gaatacccgg aaaaaattta ccaggcactg cgcaaaagct cgatgctgat
     7681 tctcgagggt gaggtgcgcc gggaaaaaat gcgccagccc gatatgacgc tgacttacct
     7741 gccggcaaaa cagaaaatca cagatctgct ctctacccac gagcgcagcc agctgaaaaa
     7801 gatgaccgat acgttgggtg tcccgatggg acagtttgag cattttcagc cgtggtttat
     7861 tgaattcatg ttcggctacc ggcttgccta caaccagggt tatgtgctcg agtacggcac
     7921 cgaacaccgc ctgctgcgct atgtcgagcg gcagctgccg gcgaaagagc gcccgcgtat
     7981 ttttaccctc gagcggttcg atgaagtgat cgtgaacatg tcgcgcctgc cgcttgccga
     8041 ccagatgaaa cgctttcgcg cgtttctcag ctattctgac cgcgtgggtg aaagtaacgc
     8101 actcgaaatg gctgaccact ggcgcaacgg cgacgatgaa aaggtgctcg aaatttttca
     8161 ctactacaac aaacagaacg cttcgacatc tgatgcgtac gcgaaggtat tgttgttcga
     8221 ccgcaaccgg cgcatggccg agcggctcga cctggtggcg aggtacccgg ggcaatattt
     8281 tgtgatctcg ggcgccttgc atctgatcgg cgagaagaat attctcgaat acctgcgagc
     8341 cgccggtttc accgtggagc gcctgtgagc agaaagtggg ggtgcgagaa gtgcgacggg
     8401 ctgggctacc gcacgcagcc gcagctgacg cgtacccgtg ccacgcagat actggcgctc
     8461 tgcgattgcg tcgaagccct ctgtcgcgtt tgcccgggcg accaggtcag cccttacgtc
     8521 tacctcgatg aaaaaagcgg cagaattctg ccctgtgact gcaagggggc acgcgacagt
     8581 tactcccgcg taaaagatct tttttcgcat tcgaatatcc cgctcaagta ccgcatgcac
     8641 cggctgcgtg agttcaacct tcagccccag tacccgaacc cgacgaaatc tcccgagctg
     8701 gaaaatgccc gccggcaggc gacgattgcg ctgacggctg cgtttgaccg ggcgcggctt
     8761 tttatcgaag cgcagaagaa actgttggcc gacccaaagc aggtgacacc agcagacctc
     8821 aagggactgc tgctcatcgg gccacccggg actggcaagt ctctgctggc ggcgatgatt
     8881 ctcaatgagc tgatcatcac cgcgcgcatg tcgtgccggt atatcaagat ctcgcgcgat
     8941 tttttccagc agttgcgcgc gaccttcagc aacgactcag gcacctacgg ccgcaccgat
     9001 tcggtattca acgagatcgc gcggcaggac gtgctggtga ttgacgactt cggtatccag
     9061 tccgattccg agtgggagca gcgcacactc tacgacctga tcgatgcccg ttacgaaaac
     9121 gacctgccga cgatcatcac gtcgaatatc gatcttgatg cggtgcgcaa tctcttcaaa
     9181 ggacgtattt actcacgctt caccgagatg ctccatacag tggagttttt ccaggtacgc
     9241 gactaccggc aggatataca cagcaatacg tgattggacg gctgggtagg gcacgctacg
     9301 gaaaagcttt accgcctgat gcgtcgatga ttatcgacgc gttatgagca ttgacaaaag
     9361 cctcctggac tttaacctct ctgacgaact cgtcgaaatt ggcgagctgg ttcgcgaaat
     9421 ctgcgaaaaa gaagtagttc cgcgccgcgc tgagctcgac gaaaaagaag cttacccttt
     9481 cgaaatcttt gccaagttca aagaagcggg tctgcccggc atcatgtacg atgcggaata
     9541 cggcggcatg ggtgtcggcc tctttgcggc gattgtgttt tctgaaattg tcagcgaata
     9601 ctgccttggt gtcaatacag ccatttcggc gaccaaactc ggcgccctgc cgattgaaca
     9661 cggtggtacc gaagaacaaa agaaaaaata cctttcggca tttgccagcg gcgaaaaaat
     9721 cggcgctttt ggcctgacag agccgaatgc cggttctgac gtccctagcc tgtcgaccac
     9781 ggctgttaaa aaaggcgatc gttatgtcct gaacggcgtg aagcagtgga tttctaacgc
     9841 cggtgttgct gatgtctaca cgatctttgc gctgaccgac aagagcaaag ggccgcgcgg
     9901 tatgtcgtgc ttcatcgtcg aaaaagacac caagggtctg tcgttcggca aactcgaaaa
     9961 caaaatgggt atccgctgct cgcatacccg ccaggtaatt ctcgaagacg tcgaagtacc
    10021 cgaagaaaac ctcgtgggtc tgaaacctaa ccgtggtttt atccacgcat tccagacact
    10081 gagttcatca cgccctgcgg ttgcggcatc agctgttggt ctcgcggtag gtgcatacaa
    10141 agaagctgtc aagtatgcac gccagcgcga gcagttcggc aagaacatca tcaacttcca
    10201 ggctgtgcaa cacatgctgg ctgacatggc gactcgcatt gaacaggctc gcctgcttac
    10261 ctatcgcgca gctaagtatg cgatggcggg tcaccctgaa gcggcgaagt tctcagcgat
    10321 ggcgaaatac gcagcttctg aaacagcagt agcagtggcg aatgacgcgc tgcagctgca
    10381 cggtggttac ggcttcacca aagactaccc ggttgaaaag atgtaccgcg acgcgaaaat
    10441 cctcacgatc tacgaaggca ccagccagat tcagaagaac gaaatcggcg cgt
//
LOCUS       DAOQJF010000079        10491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3287_length_10491_cov_4.972116, whole genome shotgun sequence.
ACCESSION   DAOQJF010000079 DAOQJF010000000
VERSION     DAOQJF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3287_length_10491_cov_4.972116"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /gene="fusA"
                     /locus_tag="PLF85_05945"
     CDS             <1..1467
                     /gene="fusA"
                     /locus_tag="PLF85_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNE19238.1"
                     /translation="YHNALVSAVAEVNDTLTEKYLETGTLSKEEIVAGLREGTIAMKL
                     FPVLCGSSFKNKGVQTLLDAVVAYLPSPLDIPPVKAFTVDQDVSEEPAMIRKADDSEP
                     FAALAFKIMADPYIGKLTYFRVYSGTLKKGSYVYNSTKGNKERIGRLLQMHANSREDI
                     EEVATGEIAAAVGLKDTSTGDTLCLEDNPAILEKMNFPAPVISVAVEPKTKADQEKMG
                     IALGRLAEEDPTFRVHTDEETGQTIIQGMGELHLEIIVDRMKREFKVEANVGKPQVAY
                     RETIRKAVEQEGKYIKQTGGRGQYGHVWIKVEPLPPGGGYVFENKIVGGVVPREYIQP
                     VNKGIEEALSSGVMAGYPVVDVKVELFDGSYHDVDSSELAFKIAGSMAFKDACRKASP
                     VLLEPIMKVEVVTPEDYMGDVVGDLNRRRGKVNAMNQRGNARVVDALVPLGEMFGYAT
                     ELRSITQGRASYTMQFEHYEEVPPNISKEISEKAASKK"
     gene            complement(1472..2569)
                     /locus_tag="PLF85_05950"
     CDS             complement(1472..2569)
                     /locus_tag="PLF85_05950"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNE19239.1"
                     /translation="MKKKFLLSALALFSLTAIINFRWLLYLVHVSAHQAKVILKSESI
                     EQRLLRADLPPEERKLLLATQKIRSFAEKAYGLKSSKSYRTYFDLGRKYLGYNITVTP
                     EFSLKPKSFSFWPIGSFDYLGFFSREMAEDWVARYRVDNADVHLSEIGGYSTLGWFED
                     PLYSTQLAWGEFALARLLAHEIAHERLYFSNDTTTSEILASYIERRLALDYLAVSGHK
                     VPSDAEIAAARRRAAAFAAEIEATREKLERLYSSAIPAEDMRRQKQQVFTALHQALTK
                     KHSPYAGLPALREIPQAGWLGNAWLVQFHRYSPQSSALDALYLQCGREARENKYQCWF
                     EKLEKLRTCSNEQRKEWLKGNGSIPEGYCRP"
     gene            complement(2612..2848)
                     /locus_tag="PLF85_05955"
     CDS             complement(2612..2848)
                     /locus_tag="PLF85_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19240.1"
                     /translation="MKKILLLSLLSFVLPMGLSTALSANNVCANCSSGSSNGCQQCRM
                     GKDSSDARKRCEKMGCKISGTSSCSSAANVKVCG"
     gene            complement(2963..3640)
                     /locus_tag="PLF85_05960"
     CDS             complement(2963..3640)
                     /locus_tag="PLF85_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19241.1"
                     /translation="MVKYTLLAGILSATALLAAPKAENTLTGGVVIEADPCQPNGPVD
                     VMTDERGVTAVDAANGSVIYRFEGEWLRKTAACGNTALALTDKHLLFYSQDTGRWLVQ
                     AVVQSDQVIGVTRGYNFSLGRDMAVFATLNEAWFFRGAKKLVHISFSGDRLRGVAAFG
                     DAGCIVTDRRTYCLGSKSETGRIYELSDMHIRNMRALSGKIIMRAPEKTLIYFPDRDQ
                     YEFTPQR"
     gene            3807..5618
                     /gene="lepA"
                     /locus_tag="PLF85_05965"
     CDS             3807..5618
                     /gene="lepA"
                     /locus_tag="PLF85_05965"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNE19242.1"
                     /translation="MPDIPRANIRNFSIIAHIDHGKSTLADRLLALGKLTDERTGKDQ
                     ILDSMDIERERGITIKSNSASFYWKGTDGQTYLFNLIDTPGHVDFTYEVSRSLAACEG
                     VLLLIDATQGVQAQTLANFYLALEADLYILPVINKIDLPSADVEKTRKQIEKSLGLSG
                     EEAVPVSGKSGINVEALMQAIIDKIPAPRVPDEAQHARALVFDAYFDTYRGVVEKIRV
                     FDGEIKKGDQISFMRKGGVHPVNEIGISQLRLVPQDSLKAGEVGYIVTGIKNVSEVML
                     GDTITLAAKPAQEPLFKYKEVKPMVFSGLFPVNGDDYPVLKESMDKLALNDPALIYEP
                     ESSEALGFGFRVGYLGLLHMEIVQERLEREFGLALLTTAPSVTYHIYFQHKPMMTLNN
                     PAEFPDPGEIDRIEEPYVKVSIISPSEYMGAILQLLADKRGENVSLNYLDPTTVQIIY
                     LVPLAEMIFEFYDRLKSVSRGYASLDYEFAEYRRSSLVKMDILVHGNRVDALSMIVHK
                     SAAETRGRQVIEQLKEHISKHQFQIAIQAAIGGKVIARETISALRKNVTAKCYGGDIS
                     RKRKLLDKQKEGKKRMKMIGSVEIPQEAFLSVLKTSD"
     gene            5633..6337
                     /locus_tag="PLF85_05970"
     CDS             5633..6337
                     /locus_tag="PLF85_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNE19243.1"
                     /translation="MIFRQIETAAGEVTLCFTGTDRDLSADSFTQVYRNIRQKLGNSF
                     AIAWAEQVHGDIVLTVENSGEPVSCVGEGDAIITQVAGIAPLVRTADCIPILFYSENS
                     PLVGAVHAGWRGLKKGVLSRAVSAVLGGDTKITDLRFVIGPFIRENSYEVGSDVAGQF
                     DARFSTPKPDGKFLLNLKSILQAEMSDLGIIPQQVSWYDADTLSSPEWFSARRGDAGR
                     NMALVFFWQAPEKGAS"
     gene            complement(6334..7428)
                     /locus_tag="PLF85_05975"
     CDS             complement(6334..7428)
                     /locus_tag="PLF85_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19244.1"
                     /translation="MVRALLLPGAFFAGSLALAAQAPLLQGTDNSAKSVRAQLTEKPA
                     DCALVAENAARLISQGPEKTLYIIAEWETLCRDSEGWRLFELSRKIHTVDARSAELTP
                     RLWQQMRYSPYLAAGVSADGEWARLVTENARRAEARSADGKLIKNWYLAGSHAVRNET
                     ARTPTATLHRLYLDERREKEENLNFSLAVTTGAWIPSGNLARLGNHPWIGYHFGAGYA
                     RFTAAIALDFRFGGTPEDYRFLNPNTGQIENTNVFFGLNVGGDLRYEFLQLGDFAFFV
                     AGGMGYDMITHYVARRYSGQRPAYSESLSLNAGLVLRWYFTEERGGFLEAMLRYHSVS
                     MGTAGEGGDDLSGSYITAGAAAGYRIVFDN"
     gene            7609..>8117
                     /locus_tag="PLF85_05980"
     CDS             7609..>8117
                     /locus_tag="PLF85_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009523 - photosystem II [Evidence IEA];
                     GO_component: GO:0009654 - photosystem II oxygen evolving
                     complex [Evidence IEA];
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNE19245.1"
                     /translation="MKKITAFALAALFVAGQVQAQSMKTHTDRKAGFSIKQPTGWKLT
                     KDRDGVNATIATKDNLAMVQVIRADVDAGTTTDAFLHEVEKQIGETHVNQLPEDKRHA
                     SPEDLANMNAEEASAGYYDLEHSGQKIHQFIMVIRKATMVYAITVTFADMADQKIKDL
                     AIKIADSVK"
     assembly_gap    8118..8217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8218..9179)
                     /gene="nadA"
                     /locus_tag="PLF85_05985"
     CDS             complement(<8218..9179)
                     /gene="nadA"
                     /locus_tag="PLF85_05985"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNE19246.1"
                     /translation="MTDQLIATWREKAGKVYLPQELDEREHLVREILQLKKQKNAVIL
                     GHNYMTPDVYHGISDFVGDSLELARRSKESNASIILFNGVHFMAETAKILNPGRKVLI
                     ADLKAGCSLAESMTGDDVRALRKKYPGVPIVSYINCTAEVKAEVDIICTSANAVRIVE
                     SLPSDTVLVVPDGYLAQNIQKHTKKKVIPWAGKCMVHELYTELDVDMARRSFPQAKIV
                     AHPECKDTVTAKVDFTGSTSQMGKYIRESGSKEVLLMTECSMGDNLRSEFPEVTFISS
                     CQTCPHMKRITLEKVRDALKYEQFEVTLSEETISRARASLDRMLA"
     gene            complement(9234..9974)
                     /locus_tag="PLF85_05990"
     CDS             complement(9234..9974)
                     /locus_tag="PLF85_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19247.1"
                     /translation="MLRITLRLMTFASPFLALLPLSAAGVAMPLVDAVNTHTAVTLKR
                     GEFNVAGTLYDGGGIMNRNILGVHDNIYLGVAFDAENAIGEQNAKFNIPGVLAKAKLT
                     DGWADFPILLAIGYDAFYAGSRGKVQTDNPYNRMIFGPYFTITKPIYLFGEEQHVHAG
                     LRMPIQPVYTPEDTEMYFGVDFPIGQFVPIFEIQRIVFDSNRLKETLFNLGLRFHIFE
                     HLAIEVDLIMGIGQKTNRMIVLEYLDRF"
     gene            10043..>10491
                     /locus_tag="PLF85_05995"
     CDS             10043..>10491
                     /locus_tag="PLF85_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19248.1"
                     /translation="MARITDISDPAYDTPPEGFYGQPRRSPRNRTLLISAGVLLLVAA
                     IASGVYYMLWQKKQTLDQTRLKAATDFEDAASRKMAPDQLPQDLELRQAVEMYNRGYL
                     KPAQAAFQQIVESSKPAEVKSMALTYLGIMADDEGKFNLAADFFDRA"
BASE COUNT         2354 a         2764 c         2969 g         2304 t
ORIGIN      
        1 taccacaatg ctctggtttc tgccgttgca gaagtcaatg acactcttac agaaaaatat
       61 ctcgaaacag gaaccctctc gaaagaagaa atcgttgctg gtctgcgtga aggcacgatt
      121 gcgatgaagc tgttcccggt gctctgcggt tcatctttca agaataaggg cgttcagacg
      181 ctgctcgatg cagtcgttgc gtacctgcca agcccgctcg atattcctcc tgtgaaggca
      241 ttcactgttg atcaggatgt ctctgaagag cccgcgatga tccgcaaagc ggacgacagt
      301 gagccatttg ctgctctcgc tttcaaaatc atggctgacc cgtatattgg taagctgacg
      361 tacttccgtg tttattcagg taccctcaag aagggttctt atgtctataa cagcacgaaa
      421 ggcaataaag agcgtattgg tcgcctgctg cagatgcacg cgaattcacg ggaagatatt
      481 gaagaagtag caaccggtga aatcgccgca gctgtgggtc tgaaagacac atcaacgggt
      541 gatactctct gcctggaaga taacccggca attctcgaga aaatgaactt cccggcaccg
      601 gttatctcgg tagcggtgga gccaaaaact aaagctgacc aggaaaagat gggtattgcc
      661 ctcggtcgcc tcgcagaaga agaccctacc ttccgcgtcc atacggacga ggagaccggt
      721 cagacgatca ttcagggtat gggcgagctc caccttgaaa tcatcgtcga ccgtatgaag
      781 cgtgagttta aggtcgaggc gaacgtcggt aagccgcagg tggcataccg cgagacgatc
      841 cgcaaggcag tcgagcagga aggcaagtac atcaagcaga ccggtggtcg cggccagtat
      901 ggtcacgttt ggatcaaggt cgagccgctg cctcctggcg gtggttatgt tttcgagaac
      961 aagatcgttg gtggtgttgt tccccgcgaa tacatacagc cggttaacaa gggtattgaa
     1021 gaagcgctgt catcaggtgt gatggctggg taccctgtag tagacgtaaa agttgaactg
     1081 ttcgacggtt cataccacga cgtggactca tcagaactcg cgttcaagat cgcaggttca
     1141 atggctttta aagatgcctg ccgcaaggca agcccggttc tgcttgagcc gatcatgaaa
     1201 gtcgaagtgg taacgcccga agattacatg ggcgatgttg tgggtgacct caaccgccgc
     1261 cgtggtaagg taaacgcgat gaaccagcgc ggtaatgcgc gtgtcgtcga cgccctggtg
     1321 ccgctggggg agatgttcgg ttacgcaact gaactccgca gcattacaca gggtcgtgca
     1381 tcttacacga tgcagttcga gcactacgaa gaagtaccac ccaacatctc gaaagagatc
     1441 tccgaaaagg cagcgtcgaa gaaataagtc gctaggggcg gcagtaacct tctggtatac
     1501 tgccgtttcc cttcagccat tcctttctct gctcgttcga gcaggttctc aatttctcca
     1561 gcttttcaaa ccagcattgg tatttgtttt ctctcgcttc ccggccgcac tgcaagtaca
     1621 gggcatcaag ggcgctgctc tgcggtgagt aacggtgaaa ttgcaccagc cacgcgttac
     1681 ctaaccagcc agcttggggt atttcccgca gagcgggcaa accggcgtaa gggctgtgct
     1741 ttttcgtcag cgcctggtgc agagcggtga agacctgctg tttctggcgg cgcatgtctt
     1801 cggccggtat tgcgctcgaa tacaaccttt cgagtttttc acgtgtagct tctatttccg
     1861 ccgcaaatgc tgctgcccgc cttcgtgcgg cagcgatttc tgcgtcgctg ggcaccttat
     1921 ggcctgagac agcaaggtag tcgagggcga ggcggcgttc tatataagaa gccaggattt
     1981 ccgaagttgt ggtgtcattg ctgaagtaga ggcgttcatg cgcaatttca tgcgcgagca
     2041 gccttgccag cgcaaactct ccccaggcga gctgcgtgga atagagcggg tcttcaaacc
     2101 agccaagcgt tgaataaccg ccgatctctg acaggtgtac atccgcgtta tccacacggt
     2161 aacgcgcgac ccagtcctca gccatctcgc gcgaaaaaaa tcccaggtaa tcaaagctgc
     2221 cgatcggcca gaaagaaaac gatttgggtt tcagcgaaaa ttcaggagta acggtaatat
     2281 tataaccgag gtatttgcga ccgaggtcga aatatgtgcg ataacttttt gacgacttta
     2341 ggccgtaagc cttttctgcg aagctgcgga ttttttgcgt ggcgagaagg agtttgcgtt
     2401 cttctggcgg caggtctgcg cggagcaggc gctgctcaat cgattcggat ttcaaaataa
     2461 ctttcgcctg gtgcgctgaa acgtgaacaa ggtagagcag ccagcggaag ttgatgatcg
     2521 ctgtgagcga gaacagagct aaggcggaga gcaggaattt tttcttcata ggcagaccga
     2581 ccggaaagct tgctttccgg tcggctggaa attaaccgca gaccttgacg tttgcagcag
     2641 aagagcagct gctggtgccg ctgatcttgc agcccatttt ttcgcagcgt ttgcgcgcgt
     2701 ctgaagaatc tttgcccatg cggcactgtt ggcagccgtt cgacgagcca gaactgcagt
     2761 tcgcgcagac attgtttgcc gaaagtgcgg tgctcaggcc catgggcaga acgaaagaga
     2821 gaagagagag tagaagtatc tttttcatgt tgtcctccga tttaccccgt tagggggtag
     2881 ggtacagcat aatcggacgg ctgacatgcg acaagctttt ttaagagctt catccgggtg
     2941 gttgaaaatg ggtatgtaac ggtcagcgct gcggagtaaa ctcgtactgg tcgcggtcag
     3001 ggaagtagat gagggtcttt tccggcgcgc gcatgatgat ttttcccgac agcgcgcgca
     3061 tattacggat gtgcatgtca ctgagctcgt agatgcggcc agtttcgctc ttgctgccga
     3121 ggcaataggt ccggcggtcg gtcacgatac agcctgcatc gccgaaggcc gcgacccccc
     3181 gcaggcggtc tcccgagaaa gagatgtgga ccagcttctt cgcgccgcgg aaaaaccatg
     3241 cttcgttaag ggtggcaaat actgccatgt cgcggcccag gctgaaattg tatccccggg
     3301 tgactccaat gacctggtcg ctctgcacca cggcctggac cagccagcgt cctgtatcct
     3361 gcgagtagaa cagcaggtgc ttatcggtca gcgcaagagc agtgttgccg caggccgctg
     3421 ttttacgcag ccattcgccc tcgaaacggt agattacact gccattggca gcatcgaccg
     3481 cggtgacacc gcgttcgtcg gtcatcacat cgaccggacc attgggctgg cacgggtcgg
     3541 cctcaatgac gaccccgccg gtaagggtgt tttccgcctt gggtgccgcg agaagtgcag
     3601 tggctgaaag tatgccagcg agcagcgtat atttaaccat cggggtaatg cggtttttta
     3661 cgcacaggca tgcgaggatt tttttacttc ggccttgcct gtgcccccgg ctgcacggca
     3721 gacgtggtaa ccgaaaggag accgcgaaga gtgaatgtcc tttaccgtct gtttggaagc
     3781 tccattcagt tcgccgtaaa gaccttatgc ctgatatccc gcgcgccaac atacgcaatt
     3841 tctcgatcat tgctcacatc gaccacggta aaagtacgct tgccgaccgg ctgctcgcgc
     3901 tcggcaagct gaccgacgaa agaaccggca aagaccagat tctcgacagc atggatatcg
     3961 agcgcgagcg cggcatcacg atcaagagta actccgcatc attttactgg aaaggcaccg
     4021 acgggcagac gtacctcttt aacctgatcg ataccccggg ccacgtcgac tttacgtacg
     4081 aggtttcgcg gtcgctcgct gcctgcgagg gcgtgctgtt gctgatcgac gcgacgcagg
     4141 gtgtgcaggc gcagacgctg gccaactttt acctcgcgct cgaagccgat ctctatattc
     4201 tgccagtcat caacaagatc gatttacctt cggctgatgt cgaaaagaca cgcaagcaga
     4261 ttgaaaaatc actgggctta agcggtgaag aagcggtgcc cgtatcgggt aaatcgggca
     4321 tcaacgtcga agcgctgatg caggctatta tcgacaagat accggctccc agggtgcctg
     4381 atgaagcaca gcacgcgcgg gcactggtgt tcgatgctta cttcgatacc taccgcggcg
     4441 tggtcgagaa gatccgcgtc tttgacggtg aaatcaaaaa gggcgaccag atctctttta
     4501 tgcgcaaggg tggagttcac ccagtgaacg agattggtat ttcgcagctg cgcctcgtac
     4561 cccaggattc gctcaaggcg ggcgaagtag gctacatcgt tactggtatc aagaatgttt
     4621 ccgaggtcat gctcggcgac accattaccc ttgccgcaaa accagcgcag gagcctctct
     4681 tcaagtacaa agaagtgaag ccgatggtct tctcgggcct ctttccggtg aatggcgatg
     4741 actaccccgt gctcaaagag tcgatggaca agctggcgct caacgacccg gcgctcatct
     4801 acgagcccga aagttctgaa gcgctcggtt ttggtttccg ggtaggttac ctcggcctgc
     4861 tgcacatgga aattgtgcag gagcggcttg agcgcgagtt cggtctcgca ctgctcacca
     4921 ctgcgccttc ggttacctac catatctact tccagcataa gccgatgatg acgctcaata
     4981 acccggcgga gtttcctgac cccggtgaaa ttgaccgtat tgaagagcct tatgtcaaag
     5041 tttcgatcat ctcgccgtca gagtacatgg gagcgattct ccagctgttg gccgacaagc
     5101 gcggcgaaaa tgtttcgctg aactaccttg acccaaccac ggtacagatc atctatctcg
     5161 tgccgctggc ggaaatgatc ttcgagttct acgaccgcct gaaaagtgtc tcgcgcggtt
     5221 atgcgagcct tgactacgag tttgccgagt accgcagaag ctccctcgtc aagatggata
     5281 ttcttgtaca cggtaaccgc gtcgacgcgc tgtcgatgat cgtgcataaa tcggcggctg
     5341 aaacccgcgg ccggcaggtg atcgagcagc tcaaagaaca tatctcgaag caccagttcc
     5401 agatcgcgat acaggcggca atcggcggca aagtgatcgc gcgcgaaacg atcagcgcac
     5461 tgcgcaagaa cgtgacggca aagtgctacg gcggtgacat cagccgtaag cgcaagctgc
     5521 tcgataagca gaaagaaggc aagaagcgca tgaagatgat cggctcggtc gaaattcctc
     5581 aggaagcttt cttgtcggtg ctcaagacca gcgactgacc caactccact ccatgatctt
     5641 caggcagatc gaaacagctg caggcgaggt gactctctgt ttcaccggca ctgaccgtga
     5701 tctctctgcc gattctttca cgcaggttta ccgcaacatc cggcaaaagt tgggcaacag
     5761 ctttgctatt gcctgggcag agcaggttca cggtgatatc gtgctcaccg ttgaaaattc
     5821 tggtgagcct gtcagctgcg tgggtgaagg tgacgcgatc ataacgcagg tcgccggaat
     5881 agccccgctc gtgcgtactg ccgactgcat accaatactg ttttactcgg aaaattcacc
     5941 gcttgtgggt gccgtgcatg ccggttggcg cggcctgaag aagggcgtgt tgtcgcgtgc
     6001 agtgtccgct gtgctcggcg gcgacacaaa aatcactgat ctgcgtttcg tcatcggacc
     6061 gtttatccgg gaaaactcgt acgaggttgg cagtgacgtt gcagggcagt ttgatgccag
     6121 gtttagcact cccaaaccgg acggcaagtt cctgctgaac ctaaagagca tcctgcaggc
     6181 agagatgagc gacctgggaa taataccaca gcaggttagc tggtatgatg ccgatacgct
     6241 cagctcgccc gagtggtttt ctgcgcgccg tggtgatgcc ggccgcaaca tggcattggt
     6301 ttttttctgg caggctcctg aaaaaggagc cagctagtta tcaaaaacga tacggtaacc
     6361 ggctgcagcg ccggcggtga tgtagctgcc cgaaaggtcg tcgccgcctt caccggcagt
     6421 gcccattgag acactgtgat agcgcaacat ggcttccagg aatcccccgc gttcttcggt
     6481 gaaataccag cgcagaacaa ggccggcatt gaggctgagg ctttcgctat aggcggggcg
     6541 ttgccccgag tagcgcctgg caacgtaatg ggtgatcata tcgtaaccca tgccgccggc
     6601 gacgaagaag gcaaagtcac cgagctgtag aaattcatag cgaagatcgc cgccgacatt
     6661 caggccgaaa aaaacattag tattttcgat ctggccggtg ttggggttca gaaaccggta
     6721 atcttcaggc gtgccgccga aacgaaagtc gagcgcgata gcggccgtga agcgcgcata
     6781 cccggcgccg aagtggtagc cgatccatgg gtggttgccg agccgggcca ggttgcccga
     6841 gggtatccat gcgccggtgg tgaccgccag tgaaaaattc aggttctctt ctttctcgcg
     6901 gcgttcgtca aggtaaagcc ggtgcagcgt tgccgtggga gtgcgtgcgg tttcgttcct
     6961 gactgcgtgg ctgccagcaa ggtaccagtt cttgatgagc ttgccgtcag ccgagcgggc
     7021 ctcggcacgc cgcgcatttt ccgtgacgag gcgtgcccat tctccatcgg cactgactcc
     7081 tgcagcgagg taaggcgagt agcgcatctg ctgccacagg cgcggcgtaa gttctgctga
     7141 gcgcgcatca accgtgtgga tcttgcgcga gagctcaaac agccgccaac cttcgctgtc
     7201 gcggcagagt gtttcccatt ctgcgataat gtagagagtc ttttcgggac cctggctgat
     7261 caggcgggct gcattttctg ctaccagcgc gcaatctgcc ggtttttcgg tcagttgtgc
     7321 gcgcacactt ttcgcagaat tatcagtccc ctgcaagagg ggagcctgcg ctgcaagcgc
     7381 caacgaaccc gcgaagaatg cccccggcaa taacagcgca cgtaccatgc ctgtctgtac
     7441 ttccgtgact gtgtcgcgta aaggtatttt gcccgtacag tttaggtaag tggttgtaga
     7501 atttagttgc aagccacggg gcgtcggtta agatgccggc ggggttgtcc agaacgccac
     7561 gcattgccct gaagcccgcg gcattccgga cacccccaag gagaaaaaat gaagaagata
     7621 acagcgttcg cgctcgcagc gctatttgtt gcaggtcagg tgcaggcgca gtctatgaaa
     7681 acgcacaccg accgcaaggc cggcttctcg attaagcagc cgacaggctg gaaactgacg
     7741 aaggaccgtg acggtgtaaa tgcgactatc gccacaaagg acaacctggc gatggtacag
     7801 gtcatccgtg ccgatgtcga cgcaggtacc actaccgatg cattccttca tgaagtcgaa
     7861 aagcagattg gcgaaaccca cgtgaaccag ctgcccgaag acaagcggca tgcctcgccc
     7921 gaagacctgg cgaacatgaa cgcagaagaa gcttctgctg gttattacga cctcgagcac
     7981 agcgggcaga agatacacca gttcatcatg gtgatccgca aggcaacaat ggtctacgcg
     8041 atcacggtaa catttgccga tatggcggac caaaagatta aagatctcgc gatcaagatc
     8101 gccgattcag taaaaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
     8221 agcatgcggt cgagtgaggc ccgtgcccgg ctgatggttt cttcgcttaa tgtgacttcg
     8281 aactgctcgt acttcagtgc atcacgcacc ttctcgagcg taatgcgttt catgtgcggg
     8341 caggtctggc atgaagaaat gaatgtcacc tcgggaaact ctgaacgcag gttgtcgccc
     8401 atcgagcact cggtcatcag cagcacttct ttactgcccg actcacggat gtactttccc
     8461 atctgggagg ttgaaccggt aaagtctacc ttcgcggtga cggtatcttt gcactcgggg
     8521 tgcgcgacaa ttttggcctg gggaaagctg cggcgcgcca tatcgacatc gagctcggtg
     8581 tagagttcgt gcaccatgca ctttcctgcc caagggataa cctttttctt cgtgtgtttt
     8641 tgtatattct gcgccaggta gccgtcaggt accacgagga cggtatccga aggcaggctt
     8701 tctacgatac gcacggcgtt ggcgctggtg cagatgatat cgacctcggc tttgacttcg
     8761 gcggtgcagt tgatataaga gacaatcggt acacccgggt acttcttgcg cagggcacgc
     8821 acgtcatcgc cggtcatact ttctgccagc gagcagccgg ctttcaggtc ggcgatcagc
     8881 actttccggc cggggttcag tatcttcgcg gtttcggcca tgaaatggac gccattaaac
     8941 aggatgatcg aggcattgct ctctttcgag cgccgggcca gttcgaggga atctcccacg
     9001 aagtctgata tgccatggta cacatcaggg gtcatataat tgtggccaag aatgacggcg
     9061 ttcttttgtt tcttgagctg taagatctcg cgtaccaggt gctcgcgctc gtcgagttct
     9121 tggggcaggt agaccttacc ggccttttcg cgccaggtcg cgatcaattg atctgtcatg
     9181 actgcaggca aggtaatgtc tgtcgctcgc tgcggcaaat gttttaggaa tgctcagaat
     9241 ctgtcgaggt attcgagcac gatcatgcgg ttggttttct ggccgatacc catgattaag
     9301 tcgacttcga tggcgagatg ttcaaagata tgaaacctta agcccaggtt gaagagtgtc
     9361 tctttgaggc ggttcgagtc gaagacaatg cgctgtattt cgaaaatggg gacaaactga
     9421 ccgatcggaa agtcgacgcc aaaatacatc tctgtatctt cgggcgtata gaccggttga
     9481 atcggcatgc gcaggccggc atggacgtgc tgttcttcgc cgaacaggta gatcggcttg
     9541 gtgatggtga agtaggggcc gaagatcatc cggttgtagg gattgtcggt ctgcaccttg
     9601 ccccggctgc cggcgtaaaa agcgtcataa cctattgcca gcagaatcgg gaagtctgcc
     9661 cagccgtcgg tcagcttggc cttggcgagc acccccggga tattgaactt cgcgttctgt
     9721 tcgccgatcg cgttttcggc gtcaaaagcc acaccgagat agatgttatc gtgcacgccc
     9781 aggatgttgc ggttcatgat accgccgccg tcgtagaggg tacccgcaac gttgaattcc
     9841 ccgcgtttca gggtgactgc cgtatgcgtg ttcaccgcat ccaccagtgg catagcaacc
     9901 ccggccgcgc tgagcggcaa aagcgccaaa aaagggctgg caaaagtcat caggcggagc
     9961 gttattctga gcatgtctta tttaacctat cgtctatgaa ttagcactat tgaactgaaa
    10021 aatattcgcc gaaatacaga ctatggcccg catcaccgat atttctgacc cggcgtacga
    10081 cacgccaccc gaagggtttt acgggcaacc ccggcgctcg ccgcgcaacc gcaccctgct
    10141 gatttctgcc ggggtattgc tgctggtggc agccatagcc tcgggcgtct actacatgct
    10201 gtggcagaaa aagcagacgc tcgaccagac acggctcaag gctgctaccg acttcgaaga
    10261 cgccgcatcg cgtaagatgg cgcctgacca gctgccgcag gacctcgagc tcaggcaggc
    10321 agtcgaaatg tataaccgcg gttacctgaa gcccgcacag gcagcattcc agcagatcgt
    10381 cgaatcttcg aaacccgccg aggtgaagag catggcgctg acctacctcg gtatcatggc
    10441 cgacgacgag ggcaagttca acctggcagc ggattttttt gatcgcgcga t
//
LOCUS       DAOQJF010000080        10482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3291_length_10482_cov_5.702311, whole genome shotgun sequence.
ACCESSION   DAOQJF010000080 DAOQJF010000000
VERSION     DAOQJF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3291_length_10482_cov_5.702311"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..549
                     /locus_tag="PLF85_06000"
     CDS             7..549
                     /locus_tag="PLF85_06000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19249.1"
                     /translation="MRYIIVPGLGNSGPAHWQSLWQNKLPGAVRCEQHDWDTPCPADW
                     VAALNDCVSLSAEPCLLVAHRLGVALVAHWAAAFNSNTVQAALLVSPSDVDSPAHTPD
                     SVRDFAPMPLKPLPFRSIVVASSDDPFVSLARAEQFANAWQAELFVIGPCGHINADSG
                     LGDWPQGLDLLAKLSGAPRH"
     gene            complement(552..2543)
                     /gene="acs"
                     /locus_tag="PLF85_06005"
     CDS             complement(552..2543)
                     /gene="acs"
                     /locus_tag="PLF85_06005"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNE19250.1"
                     /translation="MPAKKVAKRKTATHTETKFDPKKVAPTLVKGAHIKSLDDYKKLY
                     NQSVKNPTAFWSKIAKEQLTWFKPFKTVLKSDFAKAKNQWFVGGKTNLCYNALDRHLT
                     TARKNKAAIIWEPDNPAQPSRTYTYQQLHFEVCRMANVLRAHGVKKGDRVTIYMPMIP
                     ELAMATLACARIGAVHSIIFGGFSPDSIKDRIIDAGSDFVITADTGYRGGKVIPIKAN
                     VDEALEKMSGHVRKVLVVRHTGDHINIAAGRDLWWHEEAEKVSAENKPSEQDSEDPLF
                     ILYTSGSTGKPKGVLHTTGGYGVYASFTHKIVFDIKDEDTYWCTADLGWVTGHSYILY
                     GPLINGATSLMFEGIPTFPAVDRFWQVIAKHRVSIFYTAPTAIRALMRFGDDPVKKHD
                     LSSLRLLGSVGEPINPEAWLWYHRTVGKGKCPIVDTWWQTETGGILISPIPGATPTKP
                     GSATLPFFGVQPLLVDSDGKPLKGETEGNLCIQFPWPSIMRGVYGDPDRFYNTYFSTF
                     KGYYFTGDGCRRDKDGYYWITGRVDDVMNVSGHRLGTAEVESALVLHPSVAEAAVVGF
                     PHDIKGQGIYAYVILKEGVIPSDDLRKELIAQVSKEISPIAKPDAIHFTPGLPKTRSG
                     KIMRRILRKIAAGEIDSLGDTTTLADPSVVDILVKDRQG"
     gene            2787..3665
                     /locus_tag="PLF85_06010"
     CDS             2787..3665
                     /locus_tag="PLF85_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE19251.1"
                     /translation="MALKNSLVQVAPKLLCWYGMPLLLNESQRFHLLLHDLYLHPVLG
                     DAVESLPQLNGRTTAYVFSFDHYAPIMRAHSESLQVAAARKLADYINTFCSHNSIVVT
                     RTLSPRVEEVFHQADLAFLQKDLTFEKPFFQFLREHAMPLFHRHERPDRNYLRVVPKE
                     AGEYRVFIKNLGRPSDPTMTARLVNLSLNGVGLKMQDTAYRKLDLRDAVQVTLRSPKA
                     AIHINCGFVTRINPETDEIAVNFSLHDKSFVESRDAVQLQRLVLQTLEHAARMQLSSK
                     HNELLGVDLDRLVGQG"
     gene            complement(3674..4216)
                     /locus_tag="PLF85_06015"
     CDS             complement(3674..4216)
                     /locus_tag="PLF85_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE19252.1"
                     /translation="MLKDNFTRLSKYNRVMNQRVYEAAEKLPDEIRKKDLGAFFHSIH
                     ATLNHLLWADQVWLRRFALAGYGGRTSSADLFTGLEQHNAEYGRNLYENFETLKNTRQ
                     ALDNRFVEWVLDDLSDEMLLKNLNYTNNRQQPQTQRFSDVLAHLFNHQTHHRGQVTTL
                     LCQQGIDPGWTDYIYYCKDF"
     gene            complement(4221..7712)
                     /locus_tag="PLF85_06020"
     CDS             complement(4221..7712)
                     /locus_tag="PLF85_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE19253.1"
                     /translation="MNIAAFSIKRPIFIASLVILMLTTGWVSLKRIGVDLFPDVNIPF
                     ITITTTYRGAGPEEIETLVSKKIEEEVASISGLKRVSSDNMEGVSLVMCEFYLGQDIK
                     DAEQQVRNKLGKARRDLPEGIDEPLIQRFDPADQPIVRLSIAADMPPAKLFDYVEQRI
                     KPRFEQVPNVGAVRISGGQKREIQVEVDRNLLNANQMTMTWIANNLRSYGANVPVGKK
                     ESGDKEIAFRTMGRFESLKQIENTVVSFGGDAGSALLLKRLAKVSDGIEDPKSKASLY
                     LPVDDDPYDLNGKAIDQTKLVRERKPALLVDVYKQSDANTVAVSDAALKLVEKINDDL
                     RPQAIKTLNEIKAMIRKGEKADKLAPLFQKIAEDELAVKNPTVAAKLKNLQEQVGKAP
                     KSQETLDALRELKGELKRVSAAPEIALIRNTAHWIKINVDDVRESIIIGVLLAVLVVY
                     LFLGNSRSTIITGLALPNSLLGAFILMLAMGFTINVITLLALSLSVGLLVDDAIVVRE
                     NIFRKMEEGEHPVKAAETGTQEVLLAVIATTATVIAVFLPVGFLSGMVGQFLRQLGLT
                     VVFAMLISLFDAITVAPMLSAYFGGHAHDKPNWLIRKFNKFQDWLEAMYGTVMKFTLR
                     RPLVTVGISVAVMLGNCASCGAVKKTFLPPNTQPEFLVNFEMPPGTSLEGSRKMAEEI
                     ETRLRTMPEVHLIAAVAGSYSGLANKGELGVVLVDRKWRKRETWQLKDAAREMLKDLA
                     YAKVSVNDYSAVGGGVQYPLNLNVSGENLDELDKYSQVVMAELKTIKDLSDVSADFQT
                     GKPEFQVMLDPDRMQQVGVGPAAAGNELRLHVAGGVVGKFYENGLEYDIRMRLKENQR
                     DLKTSFSQSFVPNLSQPARAIPLSILGKGEVRSGPSNISRQDRSRVIKIHANLAPGGA
                     IGNAVDEARKLLETKIKPPPGIRYIFWGQAEDLKELMENIILAFGLALLFIYLVLASL
                     YESFITPITIITAIPPAISGAFVSLAIFREQLNIFSMIGLIMLMGLVTKNSILLVDYA
                     LQRVRRGMSLGDAIFEAGKARLRPILMTSFAMIAGTLPLALGLGEAAKQRTAMGIAII
                     GGLVISTIMTLVFVPAVFGYIDRLREWIEKRFRPDYDLNLVGAHGQDAHISMPKAEEA
                     VAEVAAAPKPAKRKSTK"
     gene            complement(7800..8570)
                     /locus_tag="PLF85_06025"
     CDS             complement(7800..8570)
                     /locus_tag="PLF85_06025"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE19254.1"
                     /translation="MKNLIVRCLLLSMLSTVVFAEAEAEKLQIIDVKKGDTLSKISKK
                     YLEDPSQWPALLKYNQISSPNLIQPGMKLKVPASLGKKPAAIVVYKSGKAQYARAQEN
                     TWKEVFVKLGLYSEDQVKTGAASTVHLQLSNQTILRLQPQSFIIINKIEKNSRDAVFT
                     LQEGNLHASVESMRKSGGRLMVRTPSAVAAVRGTVFELQASAQSSSLACHEGQVDVSA
                     QKVTVEVPAGMGTFVERGKAPLKPFKLPPPPEISVTEP"
     gene            8709..9470
                     /locus_tag="PLF85_06030"
     CDS             8709..9470
                     /locus_tag="PLF85_06030"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE19255.1"
                     /translation="MLEGFRRLPLLGQNIILGVLTMAVGMPIYTVPNILYGDRPGMSL
                     ATSFDNAVPFVPWTILGYALIYVFIFLPVFTIKHRPIFFRMIAGFLLCSVVALPFFIF
                     MPVRMPRPGIPTQESLFYWGVAFNYVLDKPVNCFPSLHVANAVFATACCVRLSRSVGL
                     WGILGTLFIAISTTTLKQHFIADVVAGTAIALASYFLVVHPAIKRYAAVESAEVLVFP
                     PRTALWVFYLYLIFLGICLLLFFAGLRFPPVLPVN"
     gene            9575..10330
                     /locus_tag="PLF85_06035"
     CDS             9575..10330
                     /locus_tag="PLF85_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19256.1"
                     /translation="MKKKIAVAALVLLTAIACGKKKSTEEAPKTQARIAFAVYQVGLF
                     DTVDAKKASEWLNRGEMVTVLDTVNVPDAKDPKKSKSWAKIERTTGKQGFVDPANVES
                     KAFVTVGPLEVYNINQASGKKVATVPAGQVGFVSDEKADWVKVRFGYKVYENWSGAAD
                     ALKWADGKWAQLSQVSYDPAAIGQGIELETAMRKFNDADATRKAQGQKELEKIIADGT
                     SQFVEVARRALDGAQATPANEPAAAPAATENNP"
BASE COUNT         2190 a         2861 c         2911 g         2520 t
ORIGIN      
        1 gggagaatcc gatatattat cgtgcccggc ctcggcaact ctgggcccgc acactggcag
       61 agcctctggc agaataaatt gcccggcgca gttcgttgcg agcagcacga ctgggatacc
      121 ccctgcccgg ccgattgggt agccgcgctc aatgattgcg tgagtctctc cgcagagccg
      181 tgcctgctgg ttgcgcaccg cctgggtgtc gccctggtcg cacactgggc tgctgctttc
      241 aacagcaata ctgttcaagc cgcgctgctg gtctcgccga gcgacgtcga ctctccggcg
      301 cacacgcctg acagcgtacg cgatttcgcg cccatgcccc ttaagcccct gccatttcgc
      361 agcattgtcg tcgcgagcag cgacgacccg ttcgtgtcgc tggcccgggc cgaacagttt
      421 gccaacgcct ggcaggcgga gctctttgtt attggcccct gcggtcatat caatgcagat
      481 tcgggtttgg gcgactggcc gcagggtttg gatctattgg ctaaactcag cggcgcaccg
      541 cgccactgag tttagccctg cctgtctttc acgagtatat ctaccaccga cgggtcggcg
      601 agtgtcgtcg tgtcgcccag cgagtcgatc tcgcccgccg caatcttgcg caggatacgg
      661 cgcatgatct tgccgctgcg ggtctttggc aggcccggcg tgaaatggat tgcgtcgggt
      721 ttggcaatcg ggctgatctc cttggaaacc tgcgcgatca gctctttgcg caaatcatcg
      781 ctgggtatga caccctcttt caggattacg tacgcgtaga taccctgtcc cttgatgtcg
      841 tgcggaaaac ccacaaccgc tgcttcggcg acagacgggt gtagcaccag cgctgactca
      901 acctcggcag taccgaggcg gtggccgctc acattcatca cgtcgtcgac gcggccggtg
      961 atccagtagt agccatcttt gtcgcggcgg cagccgtcgc ctgtgaagta gtagccctta
     1021 aacgtcgaga agtacgtgtt gtagaaacga tcggggtcgc cgtagacgcc gcgcatgatc
     1081 gacggccacg gaaactgtat gcacaggtta ccctcggttt cgcctttcag cggcttgccg
     1141 tcgctgtcga caagcaacgg ttgcacgccg aagaagggca gggtcgccga accaggttta
     1201 gtcggtgtcg cgccgggaat cggcgaaatc agaataccac cggtttcggt ctgccaccag
     1261 gtgtcgacga tcgggcactt gcccttgcca acggtgcggt ggtaccacaa ccacgcttcg
     1321 ggattgatgg gttcgcccac cgaaccgagc agtcgcagcg atgagaggtc gtgcttcttc
     1381 accgggtcat cgccaaagcg catcagcgcg cgtatcgctg tcggcgcggt atagaaaatg
     1441 ctcacgcggt gtttcgcgat cacctgccag aaacggtcga ctgcgggaaa agtgggaatt
     1501 ccctcgaaca tgagagaagt agcgccgttg atgagcggac cgtacaagat gtaactgtgg
     1561 cccgtgaccc agccgaggtc ggccgtgcac cagtacgtgt cttcgtcttt gatatcgaac
     1621 acgatcttgt gcgtgaacga tgcgtagaca ccgtagccac ccgtcgtgtg cagcacgccc
     1681 ttgggttttc cggtcgaacc cgatgtgtac aggataaaga gaggatcttc gctgtcctgt
     1741 tcggagggtt tgttttcggc gctgaccttt tcggcttctt catgccacca caagtcgcgg
     1801 ccggcagcga tgttgatgtg gtctccggta tggcgcacga ccagtacttt gcgcacgtgg
     1861 cccgacattt tctcgagcgc ctcgtcgacg tttgccttga tagggatcac cttgccgccg
     1921 cggtagcccg tatcggccgt gatcacgaaa tcggaaccgg catcgatgat acggtctttg
     1981 atcgaatcag gagaaaatcc accgaagatg atcgagtgca ccgcgccgat gcgggcgcag
     2041 gcgagcgtcg ccatcgcgag ttcgggaatc atcggcatgt agatcgtgac gcggtcgcct
     2101 ttcttaacgc cgtgcgcccg gagcacgttt gccatgcggc agacttcaaa atgcagctgc
     2161 tggtaagtat atgtgcgcga tggctgcgcg gggttatcgg gttcccagat gatggcggcc
     2221 ttgttcttgc gtgcggttgt gaggtgcctg tcgagcgcgt tgtagcagag attcgtttta
     2281 cctccgacga accactggtt tttcgccttg gcgaaatcag atttgagaac ggtcttgaaa
     2341 ggtttaaacc aggtgagttg ttctttcgca atcttcgacc aaaacgccgt tgggtttttt
     2401 accgactggt tatagagctt tttgtaatca tcaaggctct tgatgtgagc acccttcacg
     2461 agtgtgggag ctactttttt gggatcaaat ttcgtctctg tgtgggtggc agtctttctc
     2521 ttcgcaactt ttttcgcagg cataattcct cagtcgcgag caaccaagcc acgtcaagga
     2581 aaacggcagc ggtgtattct tcttgcgcgc cgcattgtca tcgtgcgctg cgccgcagta
     2641 tgccaataaa gcgcgacgca tcgttcaggc ttgtactctt ctgtactcta tttttggccc
     2701 ttcccgatac tccgtgtaca tcttaacaca gacggcaggc aataccgaaa gtaattaccg
     2761 accttctgcc gttgtaaagg gggcttatgg ctctgaagaa tagtctggtg caggttgcac
     2821 caaagctgct ctgctggtat ggcatgccgc tgctgctgaa tgaatcgcag cggtttcacc
     2881 tcttgctgca tgacctgtac ctgcacccgg tactgggcga tgctgtcgaa tcgctgccgc
     2941 agctgaatgg ccgaacaacg gcctatgttt tctcgttcga tcattatgcc cctataatgc
     3001 gcgctcatag cgagagcctg caggtcgcgg cggcccgcaa gctggcagat tacatcaaca
     3061 ccttctgcag ccataacagt attgttgtca ctcgcacgct ttcgccgcgc gtcgaagaag
     3121 tgtttcacca ggcggacctg gcattcctgc agaaagacct gactttcgag aagccttttt
     3181 tccaattttt gcgcgagcac gcgatgccgc tctttcacag gcacgagcga cccgaccgca
     3241 attaccttcg cgttgtaccg aaagaagcgg gcgagtaccg ggttttcatc aagaatcttg
     3301 gccggccgtc cgatccgacc atgacggcac gcctggtgaa ccttagtctc aacggcgtag
     3361 ggctcaaaat gcaggatacc gcctaccgca agctcgacct gcgcgatgcg gtacaggtga
     3421 cattacggtc gccgaaagcc gcaatacaca tcaactgcgg attcgtcacc cgaatcaatc
     3481 ccgagacaga tgaaattgcg gtgaattttt cgctgcacga taagagtttt gtcgaatcgc
     3541 gtgatgcggt acaactgcag cgccttgtgc tccagacatt agagcacgca gcgcgcatgc
     3601 agctgtcttc gaagcataac gaattgttgg gtgttgatct tgaccggctc gtaggccagg
     3661 gctgacagct gacttagaag tctttgcagt agtagatata gtctgtccat ccaggatcaa
     3721 tgccttgctg gcagagcaaa gtggttacct gtccgcgatg atgcgtctgg tggttaaaca
     3781 aatgtgcgag cacatcggag aagcgctgtg tctggggctg ctgccggtta ttggtgtagt
     3841 tgaggttctt taggagcatt tcgtccgaca ggtcatcgag gacccattca acgaaacggt
     3901 tatctaaagc ctgccgcgta ttcttcaggg tttcaaagtt ttcgtacaaa ttgcggccat
     3961 actcggcgtt atgctgctca aggccggtaa acagatcggc ggatgatgtc ctcccaccgt
     4021 atccagccag ggcaaagcgg cgtaaccata cctgatcggc ccaaagcagg tgattcagtg
     4081 ttgcatgaat tgagtggaaa aaagctccca aatctttctt acggatttca tccggcagct
     4141 tttctgctgc ctcataaacc cgttggttca tcacccggtt gtatttcgac aaccgggtga
     4201 agttgtcttt gagcattcgg ctacttggtc gatttacgct ttgccggctt cggcgcagca
     4261 gccacttctg caaccgcctc ttcagccttt ggcatactga tatgtgcgtc ctgaccgtgt
     4321 gctccgacca ggttcaggtc atagtcaggc ctgaagcgtt tctcgatcca ttcgcgcagg
     4381 cggtcgatgt agccgaacac cgcgggaacg aaaacgagag tcatgattgt cgaaatcacc
     4441 agaccgccga taatcgcgat acccatcgcc gtgcgctgct tggccgcttc gccgagaccc
     4501 agcgcgagtg gcagggtacc cgcgatcatc gcaaacgagg tcatcagaat cggacgcaga
     4561 cgcgccttgc cggcttcaaa gatcgcatca ccgagcgaca taccacggcg cactcgctgc
     4621 agcgcatagt cgacgagcag gatcgagttc ttcgtgacga gacccatgag cataatcaat
     4681 cctatcatac taaagatgtt cagctgctcg cggaagatcg cgagtgagac gaacgctccc
     4741 gagatcgcgg gcggtatcgc cgtgataatc gttatcgggg tgataaacga ttcgtagagc
     4801 gatgcgagta caaggtagat gaagagcagc gccaggccga atgccaggat aatattctcc
     4861 atcagttcct tgaggtcctc ggcctgaccc cagaagatat agcggatgcc cggcggtggc
     4921 ttaatcttcg tttcgagcag tttcctggct tcgtccacgg cgttaccgat cgcgccgcct
     4981 ggagccaggt tcgcgtgtat tttgattacg cgcgaacggt cctgccgtga aatgttcgaa
     5041 ggaccggagc gtacttcacc cttgccgaga atcgacagcg gaatggcgcg cgccggctgg
     5101 ctcaggttgg gaacgaaact ctggctaaac gaagtcttga ggtcgcgctg gttttctttg
     5161 aggcgcatac gaatgtcgta ctcaagtccg ttctcataga acttaccaac cacgccgccc
     5221 gcaacgtgca ggcgcagctc attgccggcc gctgccgggc ctacgccgac ctgctgcata
     5281 cgatccgggt caagcatgac ctggaactct ggcttgccgg tctggaaatc agccgatacg
     5341 tcactgagat ccttaatggt tttcagttca gccatgacca cctgcgaata tttgtcgagt
     5401 tcatcgaggt tctcgccact gacgttcagg ttgagcggat actgtacccc gccaccgacc
     5461 gcgctgtagt cgttcaccga gaccttcgcg taagcgaggt ccttcagcat ttcgcgcgca
     5521 gcgtctttca gctgccaggt ttcacgcttg cgccatttgc ggtcgaccag caccacgccg
     5581 agttcgccct tgttggcaag acccgagtaa gagccggcta ccgcggcaat caggtgcacc
     5641 tctggcatgg tgcgcaggcg agtttcgatt tcttcggcca tcttgcgaga accttcaagg
     5701 ctcgttcccg gcggcatctc aaagttcacg agaaattcag gctgcgtatt gggaggtaaa
     5761 aatgtcttct tcacggcgcc acacgaggcg cagttgccga gcatcaccgc gaccgagatc
     5821 ccgacagtca cgagcgggcg gcggagcgtg aacttcatca ccgttccata catcgcctcg
     5881 agccaatcct ggaacttatt gaacttgcgg atcagccagt tgggcttatc gtgtgcatgg
     5941 ccgccaaagt aagcagagag catcggggcg acggtaatcg catcaaagag tgaaatcagc
     6001 atcgcgaata ctacggtcaa accgagctgc cgcaaaaact gtcccaccat accagaaagg
     6061 aatcccacgg gtaagaacac cgcaatcacc gtcgccgttg tggcgataac ggcgagcaga
     6121 acttcctgcg taccggtttc ggccgctttg accgggtgtt cgccttcttc catcttgcgg
     6181 aagatatttt cacgcaccac gatcgcgtca tcgaccagca gccccaccga gagcgagagc
     6241 gccagcagcg tgatgacgtt gatggtgaac cccatcgcga gcatcagtat gaaagcgccc
     6301 aggagtgagt tcggcagcgc gagacccgta atgatggtcg agcggctgtt accgagaaag
     6361 aggtaaacta cgagaacggc aagcagcaca ccgatgatga tcgattcacg cacgtcgtcg
     6421 acgttgatct tgatccagtg cgcggtattt cgaatgagcg caatttcagg cgctgcgctc
     6481 actcgtttga gctcgccctt gagttcacgc agtgcatcga gagtttcctg cgatttaggc
     6541 gctttaccca cttgttcctg caggttcttg agttttgcag ctaccgttgg atttttcact
     6601 gcgagttcat cttctgctat cttctggaag agcggtgcca gcttgtcggc tttttcgccc
     6661 ttgcggatca tcgccttgat ttcattgagg gtcttgatcg cctgtggccg cagatcgtcg
     6721 tttatctttt cgaccagttt cagcgccgca tcggatacag cgaccgtatt ggcgtctgac
     6781 tgtttgtaga catccaccag cagcgctggc ttacgttcac gtacaagttt ggtctggtca
     6841 atcgctttgc cgttcagatc gtaaggatcg tcgtccacgg gaagataaag cgatgctttg
     6901 gatttcggat cttcaatgcc atcgctgacc tttgccaggc gcttgagtag cagcgcgcta
     6961 ccggcgtcac cgccgaaaga tactacagta ttctcgattt gcttcagcga ttcaaacctt
     7021 cccatggtac ggaatgcgat ctctttgtct ccgctctctt ttttacctac gggaacgttt
     7081 gcgccatacg agcgcaggtt gttagcgatc caggtcatag tcatctggtt cgcgttaagt
     7141 aggttacggt ctacctccac ctggatctcg cgcttctggc cacccgagat gcgcacggca
     7201 ccgacgttgg gaacctgttc aaaccggggc ttaatgcgct gctcgacata gtcgaagagt
     7261 tttgccggcg gcatatcggc agctatcgaa agtcggacta tgggctgatc ggcagggtcg
     7321 aaacgctgaa taagcggttc atcgatgccc tcaggcagat cgcgcctggc tttgccgagt
     7381 ttattacgta cctgctgctc ggcgtccttg atgtcttgtc cgaggtagaa ctcgcacatg
     7441 accagtgaga ccccctccat gttgtcggag ctcacccgtt tcaggccgga aatcgaggcg
     7501 acttcttctt ctatcttttt tgaaacaagt gtctcaattt cctcgggacc ggcaccacgg
     7561 taggttgtgg taatggtaat aaaggggata ttgacgtcag gaaaaaggtc aacgccgatt
     7621 cgcttgagag acacccaacc ggtggtgagc atcaggatca caaggctcgc aataaaaatc
     7681 gggcgcttga tcgaaaacgc agctatgttc atacgttaca aaataagctc cgccgggcca
     7741 gctgcccaaa aaattaacca tttggttaac cgaattctga atgcgggcgc agcccgcgct
     7801 cagggttcgg taacgctaat ttccggcgga gggggcagtt taaacggctt gagtggagct
     7861 ttacctcttt cgacaaatgt ccccatgcct gcgggaactt caactgtgac tttttgcgct
     7921 gaaacgtcta cctggccttc atggcaggcc aggctgctgc tctgcgctga agcttgcagc
     7981 tcaaatactg tcccacgaac tgcagctaca gcagatggcg tgcggaccat gaggcgacct
     8041 cccgatttgc gcatgctttc gacactggca tgcaggttgc cctcttgcag cgtgaaaaca
     8101 gcgtcgcgtg aattcttctc tattttatta atgatgataa agctctgtgg ctgcaggcgt
     8161 agaatcgtct ggttgctcag ctgcaggtgt acagtggagg ctgccccggt tttcacctgg
     8221 tcttctgaat agagcccgag cttaacaaac acctctttcc aggtgttttc ctgtgcccgc
     8281 gcatattgcg ccttgcctga cttgtagaca acaatggcgg caggcttctt gccgaggctt
     8341 gccggcactt ttagtttcat gccaggctgg atgaggttgg ggctggagat ctggttgtac
     8401 ttgagaaggg ccggccactg gctgggatct tcgaggtatt ttttcgagat ctttgaaagg
     8461 gtatcgcctt ttttgacgtc gataatctgc agcttttcag cttcggcttc ggcgaaaaca
     8521 actgttgaaa gcatagacaa cagcagacaa cggactatca gatttttcat cggcaactcc
     8581 agaatcacga acctgccgaa gtagacgaag cgtcatgcag aattacgaca gaatctttat
     8641 cagtgcctga catgcttgtc aactttttgg tttacagact gtacggagtg ccagctcgtt
     8701 cacgttcgat gctagagggt tttcggcgtc tgccgctgct tgggcagaac attattctgg
     8761 gcgtgttaac catggcagtg ggtatgccca tctacacggt gccgaatatc ctctacggcg
     8821 acaggccggg tatgagcctg gcgaccagct tcgataacgc ggtgcctttt gtaccctgga
     8881 cgatactcgg ttacgcgctt atttacgttt ttatctttct tccggtgttt accataaagc
     8941 atcgcccgat cttctttcgc atgatcgcgg gttttctgct ctgcagcgtt gtggctttgc
     9001 cattttttat cttcatgccg gtgcgcatgc cgcgtccagg tataccgact caggaatcgc
     9061 tcttctattg gggcgtagct ttcaattatg tgctcgataa gccggtgaac tgtttcccta
     9121 gcctgcatgt tgccaatgca gtttttgcga ctgcgtgctg tgttaggctc tcgcgcagcg
     9181 tggggctctg gggaatcctg gggacgctct ttatcgcaat ctccacgacg actctcaagc
     9241 agcacttcat tgccgacgtt gtggcgggca cggcgatcgc tctggcttca tactttctgg
     9301 tggttcaccc tgcaatcaaa cgttatgcag ctgtcgagag cgcagaagtc cttgtttttc
     9361 ctccgcgcac ggctctctgg gtattttacc tctacctgat tttcctcggt atttgtttgc
     9421 tgctgttctt tgccgggctg cgctttccgc ccgtgctgcc ggtaaactga ttcttggcgc
     9481 gctgcgcaat cattgcaatt tctttgagcg ctttattccc ggttttcttt attgacggat
     9541 tgtctaatcg caaatcgcgt tgcccactat ggtcatgaaa aagaaaatag cggtagctgc
     9601 gttagtctta ctgacggcga ttgcctgcgg taagaaaaaa tccaccgaag aagcgcccaa
     9661 gacgcaggcg cgcatagcgt ttgccgtata ccaagttggt ctgtttgaca cagtcgatgc
     9721 aaaaaaagcc tcagagtggc ttaaccgggg cgagatggtt actgttcttg ataccgtgaa
     9781 tgtacccgat gcaaaagacc caaaaaaatc caaaagctgg gcaaagattg agcgcacaac
     9841 aggtaagcag ggctttgtag atcctgcgaa tgtggaaagc aaggccttcg tgaccgtagg
     9901 tccgctcgaa gtctataaca tcaaccaggc atcgggcaag aaggttgcca ctgtacctgc
     9961 gggacaagtg gggtttgtct ctgatgaaaa agccgattgg gtcaaagtcc gttttggcta
    10021 caaggtttat gagaactgga gtggtgccgc tgatgccctg aaatgggctg acggcaaatg
    10081 ggcgcagctc agccaggtaa gctacgaccc ggcagctatt ggccagggca ttgagctcga
    10141 gacggcaatg cgcaagttca acgatgctga tgcgaccaga aaggcgcagg ggcagaaaga
    10201 gctcgagaag atcatcgccg acggtacttc acagttcgtt gaagttgcgc gccgcgcgct
    10261 cgatggtgca caggcgacgc ccgccaatga acctgcggcc gcgcccgcag ccacggaaaa
    10321 taatccctga tggcccgtag caaacagcgc tctgagcccg cggtttttat cgttaacccg
    10381 caggctggcg gcggtaaatc ggaaaggcag ctgaaagagc tgctggctga aattcaggca
    10441 atttacggca acgcaaagat tgtttttact gagcgcaagc tc
//
LOCUS       DAOQJF010000081        10342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3366_length_10342_cov_5.416934, whole genome shotgun sequence.
ACCESSION   DAOQJF010000081 DAOQJF010000000
VERSION     DAOQJF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_10342_cov_5.416934"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLF85_06040"
     CDS             complement(<1..925)
                     /locus_tag="PLF85_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19257.1"
                     /translation="MPLKMPRLRHFLFFVLSAGISLPGQPVTPEDFLERAREHFERRR
                     FLNTLDSLRVVLQMTAAEDATKNRTKLEAEILSAQSLAALGRRDESANMYERAISHGY
                     ADKSALAYLGNYFEARGQWPKAREYLSAYFAADKGDAQTHIRYAVVLARLKERTQARE
                     LLAAIEPAAGGKKAEECELLEKQKKLRQSFDCFWEYRNGHPDREAGYLALYRISRQLG
                     DREKQAQSAQTLYWLFGDDTRYIWPLVEVRLAERRFYDARLLLEEVIRLKGKDSDAER
                     LLANLQRDAPEAVKKPFRATPAEMRLLEELQH"
     gene            complement(927..2474)
                     /locus_tag="PLF85_06045"
     CDS             complement(927..2474)
                     /locus_tag="PLF85_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19258.1"
                     /translation="MKNRSYTNVFVLIAIAGPFALGAQAQKTSSKDLAAEVDRLNWEG
                     IDLSNDSRYLDAADRFQKAITLDDTRSARSYHNIAYTYELAGDRNRALENYQKAVARN
                     PQQVISWQSLGRTQYQMGLYKDAVFSGETVLKLDPRNMPVQKWLPDAYSKLAEQKIYD
                     ARNDLTGDPATNPNCEARPEVIGEVGFFGSVIGAIDKQSTALNMYRTAGVTTTPAGAY
                     AEFNPLRELTFRLNGQTPYYGILQPNFYAGSQKIEMQYNFKYFFIGAGILFSQLNVAD
                     SKVPGQDASYIQNTDYTTLSDTKFGLFMGGRDDLTFFILRIYPRYLFRDATAEPKSTA
                     FDVAKIDLEYRRNLRLSLGSVIDEKQPASKGPFTDIIVKVVMDEVYITEYKPVTGTDA
                     VGHYFGTYDFSIGMEFGKLQREFDKIGTTWGFLLTQRLYFQNTNNTNLTSFGNGQGYF
                     GLNTSGATTGNAFSSYRNNSFLITLFASQLFRNRYVLREQIFFEATPGSERNHAIGAM
                     LSFGMRF"
     gene            complement(2556..2933)
                     /locus_tag="PLF85_06050"
     CDS             complement(2556..2933)
                     /locus_tag="PLF85_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19259.1"
                     /translation="MQITFFAAMEETLKKALRTGIQTLISVTEEVRHGLTVLENDIQA
                     LNRRIGESKTIETTGEKLDDLIERSVQAIREYETRATQISQQIQQYLEQVDPMARQSV
                     EELSQKLQALSQKLAAEKTPPQP"
     gene            complement(2955..3317)
                     /locus_tag="PLF85_06055"
     CDS             complement(2955..3317)
                     /locus_tag="PLF85_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE19260.1"
                     /translation="MAANGKGLTFDFKDEGSHCLVTLVGDLNMFSAPEFRVNLVKQLE
                     SGVKRFLLDLSDLAFVDSSGIGVLVSFVSMARKTEGAKIVLCGLNPQIRSIFEVTRLL
                     SVFNVVEDRPAAKQEIQA"
     gene            complement(3390..3980)
                     /locus_tag="PLF85_06060"
     CDS             complement(3390..3980)
                     /locus_tag="PLF85_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNE19261.1"
                     /translation="MKDYIRGQVKDSIAVKEAFLADEANIELLNRVAGDLIAAYRAGK
                     KTLIAGNGGSAADAQHIAAEFVSRFYFDRPALASIALTTDTSALTAIGNDYGYELLFS
                     RQIEANGSSGDIYIAISTSGNSKNVLKSLESAKKLGIKTVGLTGRSGGKMKDIVDYCI
                     CVPSDETPRIQETHILIGHILCAAVEKELFEKEYRK"
     gene            complement(3995..5065)
                     /locus_tag="PLF85_06065"
     CDS             complement(3995..5065)
                     /locus_tag="PLF85_06065"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE19262.1"
                     /translation="MATYRFKLPQNLHKERFDKVVHTLLNEQVEGGVSKAMARKLVMA
                     GAAYLAGSRCRIASKPVWAGTEVRVEYDANRAKKIDTSVVTLKRESILYERGGLLVVD
                     KPAGLPTVATLDDARHNLVAALETLLGEHTGQSRTSPYLGVHHRLDAQTSGCILFTTD
                     PARNSFVSRLFQEHLVQKEYLAVVHVTAKAPPKAWEIKDQLVRAEKKKNRYASTRKLG
                     MPDSEGSFAYSRFELLEQRGDLALISCVPVTGRTHQLRVHLSEYGLPIVGDRLYHGTG
                     GAGLRAGKDAIGASSGTGGAGLRAGKDAIGASSGTGGAEQGSRLMLHAYRLAFREEDG
                     SEVLVEAPVPEEFKRLFDLKSL"
     gene            complement(5071..6333)
                     /gene="serS"
                     /locus_tag="PLF85_06070"
     CDS             complement(5071..6333)
                     /gene="serS"
                     /locus_tag="PLF85_06070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNE19263.1"
                     /translation="MIDFQLLKTEEGIARVVESLRKRGDTASSESLVTVSRERLAVIR
                     ETEALANERNVQSKKLGELKAQGKQAEFDALRETMKEVADKLKDLKEKEAEVEERFQN
                     LLDRLPNLLDEKVPVGKDETENVVVRTVGEVPQFSFEPKPHFEIAEAESLVDFERGVK
                     ISGTRFYVYNEQIARLERRLSNFMLDLHAQAGYKERNIPLLVKDECMYGTGQFPKFMD
                     EYYRMEKDGLSLIPTGEVPLTNIYADEILSAEQLPICLTAFTPCFRREAGSAGKDTRG
                     LVRVHQFYKVELVKFVKPQDSEAEWRKLTGDAEEILKRLGLTYRVLSLCSGDMGFTSS
                     ITYDLEVWMPGLNRWLEISSCSNFKDFQARRAKIRYKNPETGKNEYVHTINGSGVAAG
                     RLIAALLEYHQTADGKIDWAAIDAKLTR"
     gene            complement(6367..7191)
                     /locus_tag="PLF85_06075"
     CDS             complement(6367..7191)
                     /locus_tag="PLF85_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19264.1"
                     /translation="MADSHVPRPANVCMSDRLSCSACCGIYNLDMTAPERRQWVEENT
                     TQFMALDLAQSDNIVRFRKQRESVTLPRRVREDTYVCPFVGFVDNGILRTGSRSERPR
                     VAREPGASLRTGCLLHPEGSPHPQIGLHAHPQNFSFYGEGICQAYDCLAKERGVHRGD
                     FFRWAESADLGAYGRLASDHTLHRSLALFVQQHADLAAFYGLLAQAYERYGVVTTSFE
                     DIEKVVPGTAAALCSSLAERVAERRGARVQGRGNSGGLFARRLAALLHHTLTACLK"
     gene            7288..7950
                     /locus_tag="PLF85_06080"
     CDS             7288..7950
                     /locus_tag="PLF85_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNE19265.1"
                     /translation="MILNRMKSPIVLLLLGSVAAAGALFARSLVIDKPTYLSALENSV
                     LDELNLARTNPQTYARILEDYRKQFQGRLVKRPGKIDLMTSEGTAAVDEAIRELKGRK
                     PMQGFRISRGMSLAAKDHVKDTGPRGSTGHSGSDGSKPFDRMNRHGKWLRSAGENISY
                     GNDEGRAVIIQLIVDDAVAGRGHRKNIYNASFEVVGIACGPHKVYGTMCVQTFAGDYQ
                     EK"
     gene            complement(7963..9498)
                     /locus_tag="PLF85_06085"
     CDS             complement(7963..9498)
                     /locus_tag="PLF85_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE19266.1"
                     /translation="MPAKPKKKSAPAKKKTTAAVPAKEIARDIIQFGKAWEYSESLES
                     SSIVKIAPKYNLFINGKFVAPKSGKYFPTINPASEQKLAEVAEGGEADVDAAVKAART
                     AYSKVWSKMPGKERAKYIYRIARLIQDHARELAVLESLDGGKPIRESRDVDVPLAAAH
                     FFYYAGWADKLDYAFPGRKLNPLGVAGQVIPWNFPLLMAAWKLAPALACGNTVVLKPA
                     ETTPLTSLFLAQILQEAELPDGVVNIVTGAGATGAAVVSHSDVNKVAFTGSTLVGKRI
                     QQALAGSGKKMTLELGGKAANIIFEDAALDQAVEGIVNGIYFNQGHVCCAGSRLLVQE
                     SIHDIVVKKLFRRLQTLRVGNPLDKNTDIGAINSKMQLNKICELADSGVAEGAVMHQK
                     QGKLPDKGFWYQPTFFTNVTSSSRIAREEIFGPVLSVMTFRTPEEAIEKANNTQYGLS
                     AGVWTDKGSKAMKMANALRAGVVWINTFNKFDPASPFGGYKESGYGREGGKQGLLDYC
                     KIE"
     gene            complement(9500..10303)
                     /locus_tag="PLF85_06090"
     CDS             complement(9500..10303)
                     /locus_tag="PLF85_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19267.1"
                     /translation="MSLTEAQQNELLTLQKLSRQYEGIIRTSRNPDQLNKAKIELKKI
                     RDKMESIAPGGVAEKFLQGAAQPQASFGKKKTIEDIGQSYPSLGKMPMNRISPNSDEL
                     EINVLGSVLTAWENTFYPALGKIKLDFALSAERDSHFTVLEGLKRQMKILCETIEDIP
                     RAVREDSKAQLKDMRTRQQRQFMFDGGGFLKKVQEFWKAIYEDIQANGPKCFNKDDHI
                     QIDTRLEASNYFQGKSVRETARFTVMFLHEAIDALNLPDLPADKNKNLR"
BASE COUNT         2097 a         2974 c         2882 g         2389 t
ORIGIN      
        1 aatgttgtaa ttcttccaac aaccgcattt cggcaggtgt cgctctaaac ggcttcttga
       61 ccgcttcggg agcatcgcgc tgcaggtttg ccagcaggcg ttcggcgtcg gaatcttttc
      121 ctttcaggcg aatgacctct tcgagcaaga gccgcgcgtc atagaaccgc cgctccgcga
      181 ggcgcacctc gaccagcggc cagatatagc gcgtatcgtc gccgaaaagc cagtagaggg
      241 tctgcgccga ctgcgcctgc ttttctcgat cgcccagctg ccggctgata cggtaaagcg
      301 cgaggtatcc cgcctcacgg tccgggtgcc cattccggta ttcccagaag caatcaaacg
      361 actggcggag ctttttctgt ttttccagca gctcgcactc ttcagccttt ttcccccctg
      421 ctgcaggttc aatcgctgct aaaagctccc tggcctgtgt gcgctctttc aaccgcgcca
      481 gcactacagc atagcgtatg tgcgtctgtg catcgccctt atcagcggca aaatacgccg
      541 acaggtactc gcgcgccttt ggccactgcc cgcgtgcttc gaagtagttg ccgaggtacg
      601 cgagcgcact cttgtctgcg tagccatgcg aaatggccct ttcgtacatg ttcgcagact
      661 cgtcgcgccg gccaagggct gcaagagact gcgcagagag aatttctgcc tcgagctttg
      721 ttctgttctt cgttgcgtct tctgctgccg tcatctgcag cacgacgcgc agtgaatcga
      781 gagtattgag aaacctgcgg cgctcgaagt gttcgcgcgc gcgttcgaga aaatcttcag
      841 gggttacggg ctggccgggc agcgagatac cggcggataa gacgaaaaaa agaaaatgcc
      901 gcagccgcgg cattttcaac ggcatatcag aacctcatgc cgaacgacag catcgcacca
      961 attgcatggt ttcgttctga accgggtgtc gcttcaaaaa agatctgctc gcgcagcacg
     1021 tagcggtttc tgaacagctg cgaagcaaag agcgtgataa gaaatgagtt gttgcggtag
     1081 gatgagaacg cattgcccgt ggttgcaccg ctggtattga ggccgaaata accctggccg
     1141 ttgccgaaag aagtcaggtt ggtgttgttg gtattctgga agtacaggcg ctgtgtcagc
     1201 aagaagcccc aggtcgtgcc aatcttgtcg aactcgcgct gcagttttcc gaactccata
     1261 ccgatcgaga agtcgtaggt gccgaaatag tgtcccaccg cgtcggtgcc ggtaaccggt
     1321 ttatactccg tgatataaac ttcgtccatc acaaccttca cgatgatatc ggtgaagggt
     1381 cctttgctgg ccggctgctt ttcgtcgatt acggagccga gactcaaccg caggttgcgc
     1441 cggtactcga ggtcgatctt ggccacgtcg aacgcggtgc tcttcggttc tgcggtcgca
     1501 tcgcggaaaa ggtagcgcgg gtagatgcgc agtatgaaga aggtcaggtc gtcgcgcccg
     1561 cccatgaaaa ggccaaactt ggtgtctgac agcgtcgtgt agtccgtgtt ctggatataa
     1621 ctggcatcct gcccgggcac tttgctatcg gcgacattga gctggctgaa gagaatacct
     1681 gcgccgatga agaagtactt gaagttgtac tgcatctcga tcttttgcga acctgcgtag
     1741 aagttcggct gcagaatacc gtagtacggt gtctgtccat tgagcctgaa ggtgagttcg
     1801 cgtaacgggt taaactccgc gtaagcgcct gccggagtcg tcgtgacgcc cgctgtgcgg
     1861 tacatgttga gcgctgtcga ttgtttgtcg atggcaccga tcacagagcc gaagaaaccg
     1921 acctcaccaa taacttcggg ccgcgcctcg cagttggggt tcgtcgccgg gtcgccggtc
     1981 aggtcgttac gcgcatcgta aattttctgc tcggcgagtt tagaatatgc atcgggcagc
     2041 catttttgta ccggcatgtt gcgcgggtcg agcttgagta cggtctcacc ggaaaagacg
     2101 gcatccttat agagccccat ctggtactgc gtacgcccca ggctctgcca cgatatcacc
     2161 tgctgcgggt tacgcgctac cgccttctgg tagttttcga gcgcgcggtt tctgtcgccg
     2221 gccagttcgt aggtataggc aatgttgtgg tacgaacgcg ccgagcgcgt gtcgtcgagc
     2281 gtgatcgcct tctggaagcg gtcagctgca tcgaggtagc gcgaatcgtt agagaggtca
     2341 atcccctccc agttgaggcg atcaacctcg gccgcaaggt ctttactgct cgttttctgc
     2401 gcctgcgccc ccagggcgaa aggcccggcg attgcgataa gcacgaaaac attcgtatag
     2461 ctgcggtttt tcatatctgg ttagacgccc tttatctatg agtatcgacc tgacgcccgg
     2521 gatttttgag cctaagtcgg cgcagccggg gtggctcagg gttgcggggg agttttttcg
     2581 gctgctagct tctgcgacag tgcctgcagc ttttgcgaga gctcttctac gctctgtctc
     2641 gccatggggt caacctgttc gaggtattgc tgtatctgct ggctgatctg tgtggcgcgg
     2701 gtttcgtatt cgcgtattgc ctgcaccgag cgctcgatca ggtcatcgag tttttcaccg
     2761 gttgtctcaa tagtcttcga ctcaccaata cgccggttga gcgcctggat gtcattttcg
     2821 agcaccgtga gaccgtggcg cacctcttcg gtcactgaaa tcagggtctg gattccggtg
     2881 cgcagcgcct tcttgagtgt ctcttccatg gcggcaaaaa aagtgatctg catgaaaagt
     2941 ctacctttac cccgtcaggc ctgaatctcc tgcttggcag ccggccggtc ttcgacgacg
     3001 ttaaaaaccg acaggagccg ggttacttcg aaaatactgc gtatctgggg gttaagcccg
     3061 cagagcacaa ttttcgctcc ttcggtcttg cgcgccatgc tgacaaaact caccaacacg
     3121 ccaatgcccg aagaatcgac aaaggcgaga tcgctcaaat cgagtaagaa gcgtttcact
     3181 ccagattcga gctgttttac aaggttcacc cgaaattcgg gtgctgagaa catgttgaga
     3241 tccccaacca gggtcacgag gcaatggctg ccctcatcct taaaatcgaa ggtcagtcct
     3301 ttaccatttg cagccatcac acgtgccgtc tcccgaacag acacaatgag tcaagtggtt
     3361 cgaaacagcc accggcggta ccgtccggat tatttacggt actccttttc gaacagttct
     3421 ttctcgaccg cggcacacag tatgtgcccg atcaatatat gggtttcctg tatgcggggg
     3481 gtctcgtccg aaggcacaca gatgcagtag tcgacgatat ccttcatctt gccgccgctg
     3541 cgaccggtca gaccaactgt cttgataccc aatttcttgg ccgactcaag ggatttcagg
     3601 acgttcttcg aattgcccga ggtcgaaata gcgatgtaga tatcgccgga gctgccgttc
     3661 gcctcaatct ggcgtgaaaa gagcagctcg tagccatagt cgttaccgat agcagtcagc
     3721 gccgaagtat cggtcgtcag tgcaatcgac gccagcgccg gccggtcaaa atagaaacgg
     3781 ctgacaaatt cggctgcaat gtgctgcgca tcagccgcgc tgccaccgtt gccggcgatg
     3841 agtgtcttct tgcctgcgcg gtaagcggcg atcaggtcgc ccgcaacccg gttcagcagt
     3901 tcaatattgg cctcgtcagc caaaaaagct tctttgacgg caatcgagtc tttgacctgc
     3961 ccacggatgt aatctttcat gtgggggaaa tatactaaag ggacttcaga tcgaaaagcc
     4021 ttttgaactc ttccggcacc ggggcctcga ccaatacctc gctgccgtcc tcttcgcgga
     4081 atgccaggcg gtaggcgtgg agcatcaggc gactgccctg ctcggcacct cctgtgccac
     4141 tgctcgcacc gatggcatcc ttgccagctc gcagcccggc acctcctgtg ccactgctcg
     4201 caccgatggc atccttgcca gctcgcagcc cggcacctcc tgtgccatgg tataacctat
     4261 cgccaacgat cggcaggccg tattcgctga ggtgcacgcg cagctggtgc gttcgcccgg
     4321 tcacgggtac acagctgata agcgcgaggt caccccgctg ctcgagcagc tcgaagcggc
     4381 tgtaggcaaa cgacccctcg ctgtcgggca tgccgagctt acgcgttgag gcataacggt
     4441 tcttcttctt ctctgcgcgc accagctggt ccttgatttc ccatgcttta gggggagctt
     4501 tcgcggtgac gtgtacaact gccaggtact ctttctgcac caggtgttcc tggaacaggc
     4561 gcgagacaaa actattgcgc gccgggtcgg tggtaaagag tatgcaaccc gaagtctgcg
     4621 catcgagccg gtggtggacg cctaagtagg gagaggtgcg tgactggccg gtatgctcac
     4681 ccagcagtgt ctccagcgct gccaccaggt tgtgccgcgc gtcgtcgagc gtggctacgg
     4741 tcggcagacc tgcgggcttg tcgaccacca gcagaccacc gcgctcgtag agaatagatt
     4801 cccgcttcag agtcacaaca gaggtgtcga tttttttcgc ccggttcgca tcgtattcga
     4861 cgcgcacttc ggttcccgcc caaacgggtt tggatgcaat acggcaacgg ctgccggcaa
     4921 gatacgccgc acccgccatc acgagcttgc gcgccattgc tttcgaaact ccaccttcta
     4981 cctgctcgtt gagcagcgtg tgtacaacct tgtcgaaacg ttctttgtgc aggttctgcg
     5041 gcaatttaaa gcggtaagtt gccaaaataa ttaccgggtg agtttcgcgt caatcgctgc
     5101 ccagtcgatc ttgccgtctg ccgtctggtg gtactcgagt aacgcagcga tgaggcgacc
     5161 tgcagcgacg cccgaaccgt tgatcgtgtg cacgtactcg ttcttgcctg tttccgggtt
     5221 cttgtagcgt atcttcgcgc gccgcgcctg gaagtccttg aagttagagc acgacgagat
     5281 ctcgagccag cggttgagcc ccggcatcca gacttcgagg tcgtaagtga tcgacgaggt
     5341 gaaacccatg tcgcccgagc agaggctgag cacgcggtac gtcagcccca gacgcttcag
     5401 tatttcttca gcatcgccgg tgagttttcg ccactcggcc tctgaatcct gtggtttaac
     5461 gaacttaaca agttcaacct tgtagaactg gtgcacccgc acgagccccc gcgtgtcttt
     5521 acccgcgctg cccgcttcgc ggcgaaagca tggggtgaat gcagtgaggc agatcggcag
     5581 ttgctctgcc gaaagaattt cgtctgcata tatattcgtg agcggcactt ctcccgtcgg
     5641 aatcagcgaa agaccgtctt tctccatgcg gtagtactca tccatgaact tgggaaactg
     5701 ccccgtaccg tacatacact cgtctttgac gagcagcgga atgttgcgct ctttataacc
     5761 ggcctgcgcg tgcaggtcga gcatgaagtt agacagcctg cgctcaaggc gcgcaatctg
     5821 ctcgttgtag acgtagaacc gcgttcctga aatttttacg ccccgctcaa agtcgaccag
     5881 cgactcggct tcggcgattt caaagtgggg tttcggctcg aagctgaatt gcggaacttc
     5941 gcccaccgtg cgcacgacaa cattttccgt ctcgtctttg cccaccggca ccttctcgtc
     6001 gagcaggttc ggcaaccggt cgagcaggtt ctggaaacgc tcttcgactt ctgcctcttt
     6061 ttctttgagg tccttcagct tgtcggcgac ctctttcatc gtttcgcgca gcgcatcgaa
     6121 ctccgcctgc ttaccctgcg ccttgagttc accgagtttt ttcgactgca cgttgcgctc
     6181 gttggccagc gcttcggttt cacgtatcac agcgagccgc tcacgcgaga cggtcaccag
     6241 gctttccgaa gaagcggtgt cgccgcgttt cctcagactc tccacaacgc gtgcaatacc
     6301 ctcttcggtc ttcagcaact ggaaatcgat catgggtaaa cccgcaacac agagacaggg
     6361 cgaaaattat ttcaggcagg cagtcagcgt atggtgaagc agcgctgcca agcggcgggc
     6421 aaatagcccg ccggagtttc ccctgccctg aacccgggcg ccgcgccttt cggcaacacg
     6481 ttcggcgagc gaactgcaga gcgcagcggc agtcccaggc acaacctttt caatatcttc
     6541 gaacgaggtg gtaacaacgc catagcgctc gtacgcctgc gcgagtaatc cgtagaaagc
     6601 cgcaagatcg gcatgctgct ggacaaaaag cgccagcgaa cggtgcagcg tgtgatcaga
     6661 cgccagcctg ccgtaagccc cgagatcggc gctctctgcc cagcgaaaaa aatcaccgcg
     6721 gtgcacacca cgttctttcg ccaggcagtc ataggcctgg cagatcccct caccatagaa
     6781 cgaaaaattc tgcgggtgtg cgtgcagccc aatctgcggg tgcggcgacc cctcgggatg
     6841 gagcagacat cctgtgcgca aggacgcgcc aggttcgcgc gcgacacgag gtcgctcaga
     6901 gcgagaaccc gtacgcaaga tcccattgtc aacaaaacca acaaacgggc aaacataggt
     6961 gtcttcgcgc acacgccggg gcaacgtcac gctttcgcgc tgcttcctga agcgtacgat
     7021 attgtcgctc tgcgcaagat cgagcgccat gaactgcgtg gtattttctt ctacccattg
     7081 cctgcgctca ggcgctgtca tgtcgaggtt gtagatgccg cagcaggcgc tacacgacag
     7141 cctgtcgctc atgcagacgt ttgcgggacg tggcacgtgc gaatctgcca tttatgctgt
     7201 aagcgcacat cctgcagcac gtgcggcaag aacaaaaatc tccgtccatt cgattgacac
     7261 ccggtggcca ttgttgtgtc actggctatg attttaaatc gcatgaaatc ccccattgta
     7321 ttgctgctgc ttggcagtgt cgcggctgcc ggcgcgctct tcgcgcgcag cctcgtgatc
     7381 gacaagccca cgtacctctc agcgctcgaa aattctgtgc tcgatgagct gaatctcgcg
     7441 cggaccaacc cgcaaactta cgcgaggata ctcgaagact accgcaagca gttccagggc
     7501 aggctggtga aacgcccggg taaaatagat ctcatgacca gcgaaggcac agctgcagtc
     7561 gacgaggcaa tacgcgagct gaaaggtcgc aagcccatgc agggttttcg catttcgcgc
     7621 ggcatgtcgc tcgcggcgaa agaccatgtc aaagatacag ggccgcgcgg ttcaaccggt
     7681 cacagcggca gcgacggatc aaagccattc gaccgtatga accggcacgg caagtggctg
     7741 cgttctgccg gcgaaaacat cagctacggc aacgacgaag gccgtgcagt cattatacag
     7801 ctgattgtcg acgacgcggt tgcaggtcgt ggccaccgca agaatatcta caatgcatca
     7861 tttgaagttg tgggcatcgc ctgcggtccg cacaaggtct acggcacaat gtgcgtgcag
     7921 accttcgcag gcgactacca ggagaaataa gcagcttcgg gcttactcga ttttacaata
     7981 atcgagcagg ccctgcttgc cgccctcgcg gccataaccc gactctttgt agccgccaaa
     8041 cggcgaagcg ggatcaaact tgttgaaggt gttgatccag acaacacccg cccgcagcgc
     8101 atttgccatc ttcatcgcct tcgacccctt gtccgtccag acgccggcag agagcccgta
     8161 ttgagtattg ttagccttct cgattgcctc ttcaggcgta cggaaagtca tgaccgaaag
     8221 caccggcccg aaaatttctt cgcgcgcgat gcggcttgaa gacgttacgt tggtaaaaaa
     8281 cgtcggctgg taccagaacc ccttgtcggg gagcttgccc tgcttttggt gcatgaccgc
     8341 accttcggca acacccgaat cggccagctc acagatcttg ttcagctgca tcttcgagtt
     8401 gattgcaccg atgtcggtgt tcttgtcgag cgggttgccc acgcgcagcg tctgcagccg
     8461 gcggaagagt tttttcacca ctatgtcgtg tatagattcc tgtaccagca ggcgactgcc
     8521 tgcacagcag acatgaccct ggttgaaata gatgccgttg acgatacctt caactgcctg
     8581 gtcgagcgca gcgtcttcaa agatgatatt ggcggccttg ccacccagtt cgagcgtcat
     8641 cttcttgcct gaaccggcaa gcgcctgctg tattcttttt cccacgaggg ttgagccggt
     8701 gaaagcgacc ttgttcacat cgctatgcga aacaaccgct gcgccggttg cacccgcgcc
     8761 ggtcacaata ttcacgacac cgtcgggcag ctcggcttcc tgcagaatct gcgccaaaaa
     8821 gagcgaagtg agcggcgttg tttcagccgg cttgagcacg accgtattgc cgcaggcgag
     8881 cgctggcgca agtttccaag ccgccatcag cagagggaaa ttccagggga tcacctggcc
     8941 tgccacgccg agcgggttga gtttacggcc ggggaaagca tagtcgagct tgtcggccca
     9001 gccagcgtag tagaaaaaat gcgctgccgc aagcggcaca tcgacgtcgc gcgattcccg
     9061 aattggctta ccaccgtcga gtgactcaag caccgcgagc tcgcgggcgt ggtcctggat
     9121 cagccgcgca atgcggtaga tgtattttgc ccgttcttta ccgggcatct ttgaccagac
     9181 cttgctgtag gcggtgcgcg ccgctttgac cgccgcgtca acgtcggctt cgccgccttc
     9241 ggcgacttcg gcgagtttct gctcgctggc ggggttgatc gtcggaaagt atttgccact
     9301 cttgggcgcg acgaacttgc cattgatgaa aaggttatac ttcggcgcaa tcttcacgat
     9361 cgatgacgat tcaaggcttt ccgaatattc ccaggccttt ccgaactgga tgatatcgcg
     9421 ggctatttct tttgccggca ctgcagcggt ggtcttcttt ttcgctggcg ctgatttttt
     9481 cttaggctta gctggcatat tacctcaggt tcttattttt gtcggccggc agatcaggca
     9541 ggttcagagc gtctatggct tcgtgcaaaa acatcacggt gaagcgcgcc gtttcgcgca
     9601 ctgactttcc ctggaagtag ttagaagcct caagccgcgt atcgatctgg atatgatcgt
     9661 ccttgttgaa gcacttaggt ccattggcct ggatatcttc atagatagcc ttccagaact
     9721 cttgcacctt tttcagaaac ccgccgccat cgaacataaa ctggcgctgc tggcgggtac
     9781 gcatgtcttt cagctgcgcc ttggaatctt cgcgcactgc gcggggaata tcttcgatgg
     9841 tttcgcagag tatcttcatc tggcgcttca gcccttcgag cacggtgaag tggctgtcac
     9901 gttcggcaga cagcgcgaaa tccagcttaa ttttgcccag cgccgggtag aacgtgtttt
     9961 cccacgcggt gagcacgctg ccgagcacgt taatttcgag ttcatctgaa ttgggagaga
    10021 tgcggttcat cggcatttta ccgaggctgg gatatgactg gccgatgtct tcgatggtct
    10081 tttttttgcc gaacgaagcc tgcggctgcg cggcgccttg cagaaatttc tctgcaacac
    10141 cgcccggtgc gatagattcc atcttgtcgc ggattttttt cagttctatc ttggccttgt
    10201 tcagctggtc cgggttacgc gaagtacgga tgattccttc gtattgccgg gatagttttt
    10261 gcagcgtcag aagttcattc tgctgcgctt cagtgagaga catagtttac tcggatgtgt
    10321 gcggcggcgt atgtgtaaag ca
//
LOCUS       DAOQJF010000082        10310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3379_length_10310_cov_5.174451, whole genome shotgun sequence.
ACCESSION   DAOQJF010000082 DAOQJF010000000
VERSION     DAOQJF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3379_length_10310_cov_5.174451"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /gene="tsaD"
                     /locus_tag="PLF85_06095"
     CDS             complement(<1..919)
                     /gene="tsaD"
                     /locus_tag="PLF85_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNE19268.1"
                     /translation="MALALAFESSCDETGVALVEDGCRVIAAPLYSQLSEHRPYGGIV
                     PEYASRAHLEKFTPLLAEILRQHPLGPGDLSYVAATTRPGLVGALLVGYQTALACARH
                     FGVPLIGVHHLEAHLAAVALSGISLPYPHLGLILSGGNSAIYRLSAPGNVAVLGDTLD
                     DAAGEALDKAAQILGLGYPGGPVIEQAARRYAESRNVNPGDEIILATENPFPYILKSL
                     PRDEISFSLSGIKTALLRAVNSPGNSAEFFAFHYQERIVEHIVRNLEHALAQHPKLPI
                     VAAGGVMANTRLRSALENLCRSKQVTLYVP"
     gene            994..2772
                     /locus_tag="PLF85_06100"
     CDS             994..2772
                     /locus_tag="PLF85_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE19269.1"
                     /translation="MFPVLFIISRFATLFAGLSERLDGVTLALLVFLPGIAELALYLA
                     IRRWLSPAIPPRFLAVAATLANAAYAFLIVTDLRVLAAFHRPLDLPLYREGTAAMPVG
                     TMLGLFHWYDWSIIALSLVSILVRRPVSVPQMIPSKIALAMAAATCAVGAFTLPGAVP
                     GYAAISPVLRIPAVAALHVAEKNTTTLVASKPAHFDPLPAGLPREMHIGRNADKNTPN
                     ILFVVLESTGARYVFDQSLTLAGKGVPMPFLQKLRGESLYLARHYATANSSPRALFSL
                     FTGLYPEPSDEFFSLKRGLRIKTWNRFLAEHRGLVVTPCVTEWYFPNGLFRNNGLREI
                     IGKSQLSFGESRTEPADARNEIQTADYFAARLKTLQEPFFSVYISFAPHYPYHDYGPE
                     WRITPGRSRLDRYVDNLRLLDEQLKKFFSALQQRGSLNNTIVVLVGDHSEAFKQHPGN
                     YIHSLHSYEENLAVPAMIWYPARLRPREINFPTSHVDLGPTLLDLLEIGHNPRDFQGV
                     SVLRNYRRPHIFAYGNEGTITVYTNEFRKVQRLRDTSCRMFDLQTDAAEKQPLACDSG
                     ADSLESATKYAAGQMVLLQQLQNKNP"
     gene            complement(2818..4332)
                     /locus_tag="PLF85_06105"
     CDS             complement(2818..4332)
                     /locus_tag="PLF85_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNE19270.1"
                     /translation="MNIAIVDVGSNNIKLEIFEVAADGHSHLVFSEKFQARLGADVFL
                     TRRLRAENTEIAIAALKQIRRITQEFKCRNTVAVATAALRECDSTDFIERVRREAQIN
                     VSIISGLEEARLVYSGVRAHTSLGQGNFLINDIGGGSTEILVCNDNDIHFVESLRLGT
                     VRLRDMFDIEAKDLVKLVEKYVQRSVEPYLDDIRRFNPEAALCTGGTARTILEILREM
                     GVSLREQLRLPMVETKDFAEVVDHLSQMSRKELARIKSIDEGRRDIILPGAALLLGLL
                     RHTRMNRFMVSPNGLRDGALADYVYNKVNKNVYLRSQTQFRETGLTTIVDKYKMDRGH
                     SEHVAELSVQLFDLFADLHRLPPETRDLLKAAAMLHDIGKFIDYSQHHKHALYILSNM
                     NLPGYDNDERDIIAHTARYHRKSNPKASHVEFQRLSGRKQDIVTKLSVLLRIADSLDR
                     SYASAITGLRARKLGERSWQLGFDSAVESQLERWAFQRKKDQFEKVFGATLELE"
     gene            complement(4384..5406)
                     /locus_tag="PLF85_06110"
     CDS             complement(4384..5406)
                     /locus_tag="PLF85_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE19271.1"
                     /translation="METCSPAQRRHNTMVTGSGEKVAHKKSSPGPIIYLFLDGVGLGA
                     ADPAINPFARFKNRFFGAHAGGHYAMPGELISTDAHMGIPGLPQSATGQTALFTGYNG
                     PQILGRHVNGYPTFTLRPYFKEKNILKVMNDAGWNATLLNSYSPMYLKRLEHPRAERM
                     LSASSLMQKHSGKPYFTTDDYLAGRSLYMDMSNWFLRKHGLDVPLARPYDNGRRLVAI
                     ARHYDLSVFEYFFTDKVGHEQAWGAAKRILSHVDEFLQGVWDELDTQRETVVISSDHG
                     NMEDLSVKTHTANPVGTWVYGQHARRFTDRVKSLMDIPRVMLDIFDLPFASDFRPSTG
                     NDYVEE"
     gene            complement(5372..7351)
                     /gene="ftsH"
                     /locus_tag="PLF85_06115"
     CDS             complement(5372..7351)
                     /gene="ftsH"
                     /locus_tag="PLF85_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNE19272.1"
                     /translation="MTPQKPGPLGSGSPNKTMKNIALILLIVIMAALTYEVFQEQQGK
                     VQEIHYSRFLELVDKQALVTTEQAPLVIQGHQISGKFNNEKNEPILFKTVIPYPGDAE
                     LLETLKGKQVKYFKGAAVEESFFWRTLMPLLPWIAIVGFFWFMMARQVQSGSNRALTF
                     GKSRPKVGPDMKNRVTFGDVAGVEEAKEELKEVVDFLKAPQKFQAIGAKIPRGVLLVG
                     PPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFAQAKRQTPCIVFI
                     DEIDAVGRLRGAGLGGGHDEREQTLNQLLVEMDGFEENEGIIIIAATNRSDVLDPALL
                     RPGRFDRQVVVDTPDLKGREAILKIHGRKVPMEEGTSMEKVARGTPGFTGADLANLIN
                     EAALLAARNDRKKVTEDDLDNARDKVLMGPERKSFFIIPEEKKIIAYHEAGHAILGEL
                     LEHSEQIHKVTIIPRGRALGITQHLPEQEKHMRSRNYWLDQLVVLMGGRLAEEIEFED
                     VTTGASNDIERATNIARRMVTEWGMSERVGPIRLSGSDAGSPFLGRDYTRKGDHSEEY
                     AKLVDTEVKRIIDTAFERGRTLLKKNKKKLDLVSQALLERETITGAELREIMSGRKLK
                     PLAPNTPSGKTDDKKPSSTGTASFGGDLLSGAAPA"
     gene            7543..7803
                     /locus_tag="PLF85_06120"
     CDS             7543..7803
                     /locus_tag="PLF85_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19273.1"
                     /translation="MPAKNDPHEDELKPKLYLASDDEEEQEEREDFEEDEEGVGGERE
                     IAVSYACEDCDYRWEVYLEEEDDELDDTQYCPMCGSANTSQI"
     gene            7889..8113
                     /locus_tag="PLF85_06125"
     CDS             7889..8113
                     /locus_tag="PLF85_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNE19274.1"
                     /translation="MSYRLNSLAIAAVLLVLLLLVNSHRVPVQILFISANFPLGGVMA
                     ACIGIGAGLTILFLSLGRSYKKLIFKAKKL"
     gene            complement(8155..9066)
                     /gene="htpX"
                     /locus_tag="PLF85_06130"
     CDS             complement(8155..9066)
                     /gene="htpX"
                     /locus_tag="PLF85_06130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNE19275.1"
                     /translation="MFKRVFLFILTNVLVVATISILLSVFGVTHYVERAGRGYGLNLQ
                     KLMVVCLVWGFVGAFISLALSKVMAKWMMGLTIIDPRNASGIEAQLVMTVHRLSRQAG
                     LTTMPDVAIYESEEVNAFATGPTRSSSLVAVSTGLIRSMSWEEVEGVLGHEVAHIANG
                     DMVTMTLLQGVVNAFVMFLSRVLAFAVSTAVSRSDDEESAFPRMLNMILTIVFDIVFT
                     LLGSMVVAAFSRYREYRADAGGASLAGKQKMIAALDRLRRTHELVDNSEAPALASMKI
                     SNKGGFLALFSTHPPLEDRIRRLQQGV"
     gene            complement(9159..>10310)
                     /locus_tag="PLF85_06135"
     CDS             complement(9159..>10310)
                     /locus_tag="PLF85_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19276.1"
                     /translation="ENFFQGRQYLLSVSLRNGRPVVRFENLLVATGNDGKDLLDSAEL
                     RAIDKLKQGERNNLLVTYAEASGRLELRLNGREQAVYILKRNAQENFTLSLARLKSAP
                     LTLFSPYRGYADNVVFSNRILGDEDVLHFGALKPYGDRYEQRTGLLLSDILDIGYSQS
                     SIVSIKPEVEQDRESPLHVDFRCADRRFTPGESDGRLPFLSYDSAAGKKCRFVQFRAK
                     FTSDNSGEKSPALKKISIEFRENPPPGKPLAPKILSARDETLEIEIAPNTELDVIRGG
                     RYIIHYGHAKLKPEGAFYFRQATFGAITQVEPIAHKVPIRLVLTNEVLAQNKKWSDSK
                     PRFKNRYPVFEKGIGYYFWVTACDNAWTDAQEHADHCSEPSVPVFARFE"
BASE COUNT         2129 a         2942 c         2886 g         2353 t
ORIGIN      
        1 cgggtacata aagcgttacc tgttttgacc ggcagagatt ttcgagtgcg ctgcgcaggc
       61 gcgtattagc catcactccg ccggcggcga cgattggcag cttcggatgc tgtgcaagcg
      121 cgtgttccag gttgcgcacg atatgttcca cgatgcgctc ctggtaatgg aaggcaaaga
      181 attctgcaga atttccaggt gaattcactg cgcgcagcag tgcagtcttg atgccgctta
      241 agctgaaact gatttcatca cggggcaggc tcttgagaat atagggaaac ggattttctg
      301 tagccagtat tatctcatcg cctggattta cattccgtga ttcggcgtag cgccttgcag
      361 cctgttcgat taccggtccg ccgggataac ccagcccaag gatctgcgca gccttatcaa
      421 gagcttcgcc tgctgcatcg tcgagcgtgt cgcccagcac cgccacattg cccggggcgc
      481 ttaagcggta gatcgccgag ttgccgcctg aaagaatcag gcccaggtgt ggatacggca
      541 gcgaaattcc cgaaagcgca accgcggcga ggtgcgcctc gagatggtgc acgcctatga
      601 gcggtacgcc gaaatggcgg gcgcaggcaa gcgctgtctg gtagccgacc aggagcgccc
      661 cgaccaggcc cgggcgcgtc gtggccgcta cgtaactcag gtcgccgggc ccaagcggat
      721 gctggcgcag aatttctgcc aggagtggcg tgaacttttc caggtgcgcc cggctggcgt
      781 attcgggtac tataccaccg tagggcctgt gttcgctcag ctggctgtag agcggggcgg
      841 caatgacacg gcaaccgtct tcaacgagcg ccacaccggt ttcgtcgcag ctcgattcaa
      901 aagccagggc aagcgccatt tggcagaact ggcagggaga gacaggccga aaaaggtttt
      961 tcggcgcgtc tcgcgcagcg gttgtcagcc agcgtgttcc ctgtactctt tattatctcc
     1021 cgctttgcga cgctttttgc cgggctctcc gagaggctcg acggcgtcac gctcgcgttg
     1081 cttgtctttt tgccaggaat tgccgagctc gcgctctacc tcgcgatccg ccgctggctc
     1141 tcgccagcga taccgccgcg tttcttggct gttgcagcaa ctctggccaa tgccgcctac
     1201 gcattcctga tcgtcaccga cctgcgcgtg ctcgcggcat tccaccgtcc gctcgaccta
     1261 ccgctctacc gcgaaggtac tgcggctatg ccagtgggta cgatgcttgg tctcttccat
     1321 tggtatgact ggagcatcat tgcgctatca cttgtttcga tcctggtccg gcgcccggtg
     1381 tcggtgccac agatgattcc ttctaaaatc gctttggcaa tggctgcagc aacctgcgca
     1441 gtgggagctt tcacactgcc cggggccgta cccggttacg cggccatctc gcctgttctg
     1501 cgcattcctg ccgtggctgc tctgcatgtt gcagaaaaaa atacgacaac gcttgttgcc
     1561 tcgaaaccag cgcacttcga cccgctgcca gccggtctgc cgcgtgagat gcacatcggc
     1621 agaaacgctg acaagaatac gccgaatatc ctgttcgtcg tgctcgaaag tacgggggcg
     1681 cgctacgtct ttgaccagtc gctgacgctg gccggcaagg gtgtgccgat gccttttttg
     1741 cagaaactga gaggagagtc tctctatctc gcgcgtcact acgcgactgc caatagctct
     1801 ccccgggcgc tcttttccct gttcaccggg ctttatcccg aaccgtcgga cgaattcttt
     1861 tcgctgaaaa gaggccttcg cataaaaacg tggaaccgct ttcttgccga acaccgcggt
     1921 cttgtcgtta ctccctgtgt gaccgagtgg tattttccga atggcctgtt tcgtaacaac
     1981 ggcctgcgcg aaattattgg taagagccag ctcagcttcg gtgaaagccg cacagaacca
     2041 gccgacgcca ggaacgagat acagactgca gactattttg ccgcacggct gaaaactctg
     2101 caggaaccgt tcttttctgt ctacatcagc tttgcgccgc attacccgta ccatgactat
     2161 ggccccgagt ggcgcatcac tcctgggcgt tcgcgcctcg accgttacgt cgacaacctg
     2221 cgcctgctgg acgagcagct gaagaagttc ttcagcgcgc tgcagcaacg cggcagtctc
     2281 aacaacacga ttgtcgtgct ggttggcgat cacagcgaag ccttcaagca acacccgggc
     2341 aactacatcc attcgctgca cagctatgaa gagaatctcg cggtgcctgc aatgatttgg
     2401 taccccgcga gactgcgacc gcgcgagatc aatttcccaa cttcacacgt cgacctgggt
     2461 cccacgttgc ttgacctgct cgagatcggc cacaacccgc gcgatttcca gggtgtctcc
     2521 gttctccgta attaccggcg gccacacatc ttcgcctacg gcaacgaggg tacgataaca
     2581 gtctacacca acgaattcag gaaggtccag cgcctacgcg acaccagctg ccggatgttt
     2641 gacctgcaga ccgacgcagc ggaaaagcag ccgttggcct gtgattcagg tgctgattca
     2701 ctcgagtccg cgacaaaata cgctgccggc caaatggtgc tgctacagca gctgcagaac
     2761 aagaatccct aacaggctcg gcctgttagg agaaacactg tggtgactcg ataagactca
     2821 ctcgagttcg agtgtagcac caaaaacttt ttcaaactgg tcttttttac gctggaatgc
     2881 ccagcgttcg agctggcttt ccactgcgct gtcaaaaccc agctgccagg agcgctcgcc
     2941 cagcttacgg gcgcgcagcc cggtgatggc actggcatag ctgcggtcca ggctgtccgc
     3001 aatacgcagc aggacagaga gtttcgtgac gatatcctgc ttacgtccgc tcagccgctg
     3061 gaattcgaca tgcgaagcct tcgggttaga cttgcggtga tagcgtgctg tgtgtgcaat
     3121 gatatcgcgc tcgtcgttat cgtagccggg aagattcata ttgctcagga tatacagcgc
     3181 gtgcttgtgg tgctgcgagt agtcgatgaa cttaccgata tcatgaagca tcgctgccgc
     3241 tttgagcagg tcccgcgttt ccggtggcag gcggtgcaga tcggcaaaca gatcgaacag
     3301 ctgtaccgat agctcggcga cgtgttcaga atggccacga tccatcttgt acttatcgac
     3361 gatcgttgtc agcccggttt cgcggaactg ggtctggctc ctgagataga cgttcttgtt
     3421 gaccttgtta tagacgtagt ccgcgagcgc cccatcgcgc aggccgttgg gtgaaaccat
     3481 aaaacggttc atgcgcgtgt gccggagcag gcccagtaga agagcagccc cgggcaagat
     3541 gatatcccgc cgaccttcgt cgatgctctt gatacgcgcc agttccttcc ggctcatctg
     3601 cgagagatgg tcgacaactt ccgcaaaatc ttttgtctcg accatcggca gccgcagctg
     3661 ttcacgcagt gaaacaccca tttcacgcag gatctccaat atggttcgag cagtaccgcc
     3721 ggtgcacagc gctgcctcgg ggttgaaccg gcggatgtca tcaaggtacg gttcgaccga
     3781 acgctgcacg tacttttcga ccagcttcac gaggtctttg gcctcaatgt cgaacatatc
     3841 ccgcaggcgc accgtaccca gccgcaggct ctcgacaaag tggatgtcat tatcgttgca
     3901 gacgagtatc tctgtcgagc cgccaccgat atcgttgatc aggaaattac cctgtcccag
     3961 cgacgtgtgg gctctcactc cgctgtaaac gaggcgcgcc tcttcaagcc ccgagatgat
     4021 cgagacattg atctgtgctt cgcgccggac tcgctcgatg aaatccgtac tgtcgcactc
     4081 gcgcaaggct gcggttgcga cggctaccgt gttgcggcat ttgaattcct gcgtgatgcg
     4141 gcggatctgt ttgagcgctg caatcgctat ttcggtattc tcggcgcgca ggcgacgggt
     4201 caggaacaca tctgccccga ggcgtgcctg gaatttttcg ctgaagacaa gatgcgagtg
     4261 accatctgca gcgacctcga agatttcgag cttaatattg ttcgacccga cgtcgacgat
     4321 ggctatattc aatgcggtta cctgtgtggt tgtcgcccgg gccgagaacg ccggcgaacc
     4381 tggctattct tcgacgtagt cgttacctgt gcttggtcta aaatccgagg caaaaggcaa
     4441 atcaaaaata tcaagcatca cccgtggaat atccatcagg ctttttacgc ggtcggtgaa
     4501 ccggcgggcg tgctggccgt acacccacgt gccgaccgga ttcgccgtat gcgttttcac
     4561 tgaaaggtct tccatgttgc cgtgatctga gctgatcacg actgtctctc tttgtgtatc
     4621 gagttcatcc cagacaccct gcaggaattc gtcgacatgg ctcaggatac gcttggctgc
     4681 gccccatgcc tgttcgtggc ctactttgtc ggtgaagaag tactcgaaga ccgaaagatc
     4741 atagtgccgc gcaatagcta ccagacggcg accgttgtcg tatggccgcg ccagggggac
     4801 atccagacca tgcttgcgca agaaccagtt gctcatgtcc atgtacaggc tgcggcctgc
     4861 gaggtaatcg tcagtggtaa agtacggttt gcccgagtgt ttctgcatca gtgaagacgc
     4921 cgagagcata cgctcggcac gggggtgctc gagccgcttc aggtacattg gcgaatacga
     4981 attgagcagc gtggcattcc agcctgcgtc gttcatcacc ttaaggatat ttttctcttt
     5041 aaaataaggg cgcagtgtga acgtcgggta gccgtttaca tgcctgccca gtatctgtgg
     5101 cccgttgtat cccgtgaaga gtgccgtctg gcctgtagca gactgcggca acccgggaat
     5161 tcccatgtga gcgtcagtgg aaatcagctc gcccggcatc gcataatgcc cccctgcatg
     5221 cgctccaaaa aagcggttct tgaaccgcgc aaatgggttt attgcagggt ctgcggcgcc
     5281 caggccgaca ccgtcgagaa acaggtaaat gattgggccg ggggagctct ttttgtgagc
     5341 taccttttct cccgaccctg tcaccatcgt gttatgccgg cgctgcgccg gagagcaggt
     5401 ctccaccgaa tgaagctgtg ccggtgctcg agggcttttt gtcatcggtc ttaccgcttg
     5461 gcgtgttcgg cgccagcggc ttcagcttgc gaccgctcat gatttcgcgc agttctgcgc
     5521 cggtaatggt ctcgcgttcg agcagtgcct gcgaaaccag atcgagtttc ttcttgttct
     5581 ttttcagcag ggtacgaccg cgttcaaacg cagtgtcgat gatacgtttg acctcggtat
     5641 cgacgagttt tgcgtattct tcagagtggt cacctttgcg cgtgtagtcg cggccaagga
     5701 aaggtgaacc cgcatcggaa ccgctcaggc gaatcgggcc gacgcgctct gacatacccc
     5761 attcggtgac catgcgccgt gcaatatttg ttgcacgttc gatgtcgttc gatgcacccg
     5821 tagtcacatc ttcaaactcg atctcttctg caagacggcc gcccatcagc acgacaagct
     5881 ggtcgagcca gtagttgcgg gagcgcatat gtttttcctg ttcgggcagg tgttgtgtaa
     5941 taccgagcgc gcgcccgcgc ggaataattg taaccttgtg gatctgttct gagtgctcga
     6001 gcaactcgcc caaaatcgca tgccctgcct cgtggtaggc gatgatcttc ttctcttcag
     6061 ggataatgaa gaacgacttg cgttcgggcc ccatcagcac cttgtcgcgg gcattatcca
     6121 gatcgtcttc tgtgaccttc ttgcggtcgt tgcgtgcagc gagcagcgca gcttcgttga
     6181 tcaggttggc gagatcggcg ccggtaaaac ccggcgtacc acgcgcaacc ttttccatcg
     6241 acgtaccttc ttccatgggt accttgcgcc cgtggatctt caggatcgcc tcacgcccct
     6301 tgaggtcagg agtgtctaca acaacctggc ggtcaaagcg gcccgggcgc agcagggcgg
     6361 ggtcgagtac atcggagcgg ttggtagcag cgatgatgat gatgccttcg ttttcttcaa
     6421 aaccatccat ctcgacgagc agctggttga gcgtctgctc acgctcatcg tggccaccgc
     6481 ctaaacccgc gccgcgcaga cgaccgacag catcaatctc gtcgatgaag acgatacatg
     6541 gcgtctggcg ctttgcctgg gcaaaaaggt cgcgcacgcg gctggcgcct acaccgacaa
     6601 acatctcgac gaagtcagaa cctgaaatcg agaagaacgg gactcccgct tcgccggcga
     6661 cagctttcgc cagcagggtt ttaccggtac ccggaggacc gacgagcagt acgccgcgcg
     6721 ggattttagc gccgatagcc tggaactttt gcggggcctt caggaaatcc acgacttctt
     6781 tgagctcttc ttttgcctct tcgacacctg ccacgtcacc gaaggtgacg cggttcttca
     6841 tatcggggcc aaccttcggc cgtgatttgc caaatgtcag cgcacggttg ctgcccgatt
     6901 gcacctgccg cgccatcatg aaccagaaga aacccacaat cgcaatccag ggcagtagtg
     6961 gcataagggt gcgccagaag aaactttctt caacggcagc gcctttgaaa tacttcacct
     7021 gcttgccctt cagtgtctcg agcagctctg cgtcgccggg atatggaatt acagttttga
     7081 acagtatagg ctcattcttt tcgttgttga acttgccgct gatctggtgg ccctggatta
     7141 ccagcggggc ctgctccgtg gtgaccagcg cctgcttgtc taccagctcg agaaagcgcg
     7201 agtagtggat ttcctggact ttaccctgct gctcctggaa tacctcgtag gtcagggccg
     7261 ccatgataac aatcagcaaa atgagtgcaa tatttttcat agtcttattc gggcttcctg
     7321 atcccagtgg gccgggcttt tgtggtgtca tcgctgtttt tctccattcg cagggaaact
     7381 cctcagattt gtgaaatcag aatttcccac tcaagtcttt ccatcgacag gcagtcaatt
     7441 tctaatgcca gtctgacttt cgttctctca caggataata aaaaacgccc attttgccaa
     7501 aaatacttga cccgcctttt gctatttggg ccacgcgtca ccatgccagc gaaaaacgac
     7561 ccacacgaag acgagttgaa gccaaagctc tacctcgcct cagacgacga agaagagcag
     7621 gaagaacgcg aagattttga agaagatgaa gaaggcgtag gcggcgagcg cgaaatagcg
     7681 gtcagctatg cctgcgaaga ctgcgattac cgctgggaag tctaccttga agaagaagac
     7741 gacgaactcg acgatacgca gtactgcccg atgtgcggca gcgccaacac gagccagatc
     7801 tgaaaacccg cgcccagagc cggcaatccg gcttaaagaa cccgctgctg ggtaaaatgc
     7861 cacgcagcgg gtccgcgaca gtcaagatat gtcttatcgc ctgaactccc ttgctatcgc
     7921 tgcagtactg cttgtcctgc tgttgcttgt caacagccac cgcgtgcctg tgcagatact
     7981 ctttatcagt gctaactttc ctttaggggg agttatggct gcctgcatcg gcatcggcgc
     8041 cgggctgact attttgttcc tcagcctcgg gcgttcgtac aagaaactga tttttaaagc
     8101 gaaaaaactg tagcagattc cgtggcgcct gcaagacgcc acggttccgg tttatcaaac
     8161 gccttgttgc aggcggcgga tacggtcttc gagtggcggg tgtgtcgaga acagggcaag
     8221 aaaaccgccc ttgttcgata tcttcatcga tgccaatgcc ggcgcctcgg agttatcgac
     8281 gagttcatgc gtgcggcgca gtcggtcgag cgcggcaatc atcttttgtt tgcccgccag
     8341 gcttgcgcca cctgcgtccg cgcggtactc acggtagcgc gagaaggcgg caactaccat
     8401 cgagcccagc agcgtgaaca cgatgtcgaa cacgatggtc aggatcatgt tgagcatgcg
     8461 gggaaaagcg ctctcttcgt cgtcgctgcg gctgaccgcg gtgctgacgg caaatgcgag
     8521 cactcgtgaa agaaacatca cgaaggcatt caccacgccc tgcagcagtg tcatcgtaac
     8581 catatcgccg ttcgcgatgt gcgcaacttc atggcccagc actccttcaa cttcttccca
     8641 gctcatggag cgaataagcc ccgtggatac ggcaaccagc gagctcgaac gggtaggccc
     8701 cgtcgcgaat gcattcactt cttctgactc gtagatagcg acgtcgggca tagtggtaag
     8761 ccctgcctgg cgcgacaggc ggtgtaccgt catgaccagc tgcgcttcaa tgccgctggc
     8821 attgcgcggg tcaatgatgg tcaggcccat catccatttg gccatgactt tcgacagcgc
     8881 gagcgagata aatgcgccga caaaacccca gacaaggcag actaccatca gcttctgcag
     8941 gttgagaccg tagccgcggc ctgcgcgctc gacgtagtgc gtaacgccga atacgctcag
     9001 cagtatcgag atcgttgcaa cgaccagtac gttggtcagt atgaacaaga aaacgcgttt
     9061 gaacatttat cctccgcggg tgcctttatg cgcacaccgt ttattacggc acaaaatatc
     9121 ttgtgcaaag gttgtcggca aattttcgct cttagcgatt attcgaagcg cgcaaacacg
     9181 ggaaccgatg gctcggaaca gtggtctgca tgttcttgcg cgtctgtcca ggcgttgtcg
     9241 catgccgtaa cccagaagta gtagccgatg cccttctcga agacagggta acggtttttg
     9301 aagcgcggct tcgagtctga ccattttttg ttctgcgcga gaacctcgtt ggtgagcacc
     9361 aaccggatgg gaaccttgtg cgcgatcggt tcaacctgcg tgatggcgcc aaaggtcgcc
     9421 tgcctgaaat agaaggcgcc ttcgggtttc agttttgcgt ggccgtaatg tattatgtaa
     9481 cgaccgcccc tgatcacatc gagctcagtg ttgggagcga tttcgatttc cagcgtctca
     9541 tcgcgcgccg agaggatttt cggcgcgagc ggtttgccgg gcgggggatt ttcgcggaat
     9601 tcgatcgaaa tctttttcag cgctggcgat ttttccccgg agttgtctga ggtaaacttt
     9661 gccctgaact gcacgaagcg gcatttttta cccgctgcgc tgtcatacga aaggaatggc
     9721 aggcggccgt cgctctcccc gggggtgaag cggcgatctg cgcagcggaa atccacgtgc
     9781 agcgggctct cgcggtcctg ttcaacctct ggcttaatcg acacgatact gctctgtgaa
     9841 tagccgatgt cgagtatatc gctcaggagc aagccggtgc gctgctcgta acggtcgccg
     9901 taaggtttca gcgcgccaaa atgcaaaacg tcttcgtcgc ccaggatgcg gttgctgaac
     9961 acaacattgt ctgcgtaacc gcggtaaggc gaaaaaagtg taagaggagc agatttgagc
    10021 cgggcgaggc tgagcgtgaa attttcctgt gcattacgct tcagaatata cacggcctgt
    10081 tcgcggccat tgaggcgtag ttcgagcctg ccgcttgctt cagcgtaggt taccagcagg
    10141 ttattgcgct ccccctgttt cagcttgtcg atagcgcgga gttcggctga atcgagcagg
    10201 tctttgccgt cgttgccggt cgcgacgagc aggttttcaa agcgcactac cgggcggccg
    10261 ttacgcagcg aaaccgagag caggtattgc ctgccctgga aaaaattctc
//
LOCUS       DAOQJF010000083        10274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3391_length_10274_cov_4.891868, whole genome shotgun sequence.
ACCESSION   DAOQJF010000083 DAOQJF010000000
VERSION     DAOQJF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10274
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_10274_cov_4.891868"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLF85_06140"
     CDS             <1..539
                     /locus_tag="PLF85_06140"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNE19277.1"
                     /translation="LSDFTALPGKVNSVEPGRKFRQNALNEEARAPGGKRPRSSMSPL
                     IMRFDDGRVVALGSPGGPTIVGTVALTAVRVLAGQELQQAVNAPRALMMPHGKALLEL
                     PLRRNKAFLQALPLAGIEADLGRKIISLGSVQAVECTPTAQKDRVTVAEKTQSMVCTA
                     ANDLRREGLGLVPNIRVD"
     gene            540..2198
                     /locus_tag="PLF85_06145"
     CDS             540..2198
                     /locus_tag="PLF85_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNE19278.1"
                     /translation="MIPNLAAADFWVIAAYFVLTFGVGVFALRRRHETKEDYFLSGRS
                     LPWHLAGLSMVATTFAADTPLAVTGITIERGIAGNFLWLSLIPSGILTAIFYARLWRR
                     SGVMTDAEFATLRYSGKPARFLRRFRALYLAVPVNLIIMGWVTTGMAKVMQVLFQFPT
                     WQTLGMLYAFTVLYIVLSGLWGVVITDVLQFLIAITACIELAVIATGHAGGLDQLWQN
                     AEKTLRPGATEIYPWYYAGPVYVVVVWLGAQWWASWYPGFEPGGGGYVAQRLLSTRNE
                     NHAVAAALLFNVLHFVVRPWPWIITALAAAVALPQGTDKELLYPQAILAWMPSGLKGL
                     MVAAFLAAFMSTIATHLNWGASYILNDVFAGTRFAPQTQRGEMLLSRITIIFLAVAAV
                     GVSFLMDSVSAGWELILSISAGTGPVYLLRWYWKRINALSEISAMAGAFLFSLLLAGF
                     DLPQEIRLCLVALFTSIFWVAVTLVTRPEGTEKLAAFEKLVSPRRWTLRELVVFVAAV
                     AVIYSAFFLIGELLRKNWYAVSLNALGLAVALAVTYAALRRRVN"
     gene            complement(2191..2703)
                     /locus_tag="PLF85_06150"
     CDS             complement(2191..2703)
                     /locus_tag="PLF85_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNE19279.1"
                     /translation="MDDKLKGKLLIASSAILDGNFNRSVVLLVDHDSRGSLGLVLNHP
                     LPGETRRHKPLFAGGPVDPEHRSLLHGADEFATGEPIIDGVYFESSDDLLPVLMQKKF
                     RYRRFCGYAGWSTGQLEYELRSNSWVLLDARPDLVFHDDGYDLWRRCLIEKGGVYRHF
                     ASTHKSILLN"
     gene            complement(2703..4682)
                     /locus_tag="PLF85_06155"
     CDS             complement(2703..4682)
                     /locus_tag="PLF85_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19280.1"
                     /translation="MSTEHKPFYIDLDEQQFRDFARGIPLMEDEALMLESYARYAAQN
                     DTISFSTARLTDFLLSRGSDENIAKLKTLPNVRRLITQTAFPLREKKFIDADLDGGEV
                     LRIRLTDPATLFLEEYYGQMLSNAALPFPDEDMVRRFLLPNNHVDIAVSEISSGKNHR
                     LKEEKRLILFRFGDMMQAVCATGNTVDMMLEICLLKLRNVIANSAQNRLAEDLVKDMQ
                     NIMPQKTSNVERIMRILSHNESEVPLYLVNLANRLAAYFTMDKDKRGMSTLLQAARLV
                     EAFKGYEAWLESEKNNKDRLRENSQKLLNMMADYPALLNREGIIQKVVKGSAGLEIMA
                     SLLNQAEIELVVQDMLSEYTVFQADERELLPALLRFRMNEEEFFIHREALLIFFESER
                     KRVRAELLSRFRKMWYALMLRNESRSSMEFDEFFAEDVEKYVKAHEPVFSTLLQNAQA
                     ILNAFHIISRHPMSANLQELYFFAGKDRIIFRPVHSLLELVRREIVAVVRAELPFLYR
                     YPLLRWLATLFGLVGSEQPAAARVEDSPPKTTVNSADTDWHRALNMAETRLIGEKNAM
                     EMIGKYAELWNIKLGDTRRQLSERVDTDIAQRAKRLYSMTRKLPEITQSFLSGEISNV
                     ATQIVRKYGNETADTRALSNYVQLSLIEQLKSIHN"
     gene            complement(4771..6510)
                     /locus_tag="PLF85_06160"
     CDS             complement(4771..6510)
                     /locus_tag="PLF85_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNE19281.1"
                     /translation="MLRALLLAVLQPLRLIVYAWFRLRALHRPFVLQLAVRGEFSEAP
                     LSHGIWAFFKPDRDRFYLLCLELQSVITAAAARRLKLSHISLTIESHNLGWAQAWELK
                     RLLESAREQGIRTSCWLIADDRVSLYIATGCEFIYAAEAATFDLSPFTSESLFMLSML
                     NKLGVRPQFLSVGDFKSAAEIFTRNNMSRAARQQTEELVADLEAEFFSAISGRAPSLG
                     RKDRPQLLSATAAMAAGLLQGTASAQEYGAWIKASEKTAMRDLSQALTLVRARAFRLL
                     PFRRPHRVALIVAEGNIVESHRPRPGTINWHDYESVADELRSGHFNAALLRINSPGGS
                     ALVSQLLWREWMLATHRLEGNKLGSPVAEAKKTKRAAKTRKERQPLPVVVSQGNVAAS
                     GGYYLSAAGESVFTTPLTITGSIGVVGGKFNVSPLLAKWGVNVDRAPKKNTAPFFSAF
                     ADFAPVQKQQLTANMHEIYEQFLRDVAQGRGMGQDKIRPLASGRVYSGKRAHTLGLAD
                     QKGGLTDALAAVREKLGLSALSPLEIAILPTVHESLFSRAGLPLGLSRLAALGDFAMP
                     GIYTLDPRWVTLA"
     gene            6734..7684
                     /locus_tag="PLF85_06165"
     CDS             6734..7684
                     /locus_tag="PLF85_06165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE19282.1"
                     /translation="MPPEAEKALARVTQNVQTFPEGADKQPPQLKRLASAGVTLNAWS
                     RDFARGSAILLEFESSDNLAQAELTVNDRPLALSRSESGIFALFANSPDSKLKQNRVV
                     LKRSGAAQAEFVLPVVSKEYPVAVSKMTVNKFSNQSKPMSQETIDAIEAGRKKKLKAL
                     VSGSGNLLTGDFRYPRDEHKITSPFFIKRVYERFRLVKGKKKKLPGKTSYHGGTDLKG
                     LKGAPIFAIADGVVNLAEHLFYEGNIVILDHGNGVISGYMHQSELVVKEGEKVKAGQL
                     IGRSGDTGMVTGPHLHIFLLIHGVKVDPLSLLVLPVRPGR"
     gene            7684..8160
                     /locus_tag="PLF85_06170"
     CDS             7684..8160
                     /locus_tag="PLF85_06170"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HNE19283.1"
                     /translation="MASGHTEFVVILLLAVVFLAAGVFIGVRISSLRAEAKFVSILQQ
                     KEAEWGQSAEAERRQALAKSRQVIGGSFSEQLAPYLPDFPFDPTEARFIGKPVDFVVF
                     RGLAAGAVEELVFVEVKSGKSRLNRNESSIRHAVAAGRVSFFEYRVPAGITSEPGD"
     gene            complement(8157..8870)
                     /locus_tag="PLF85_06175"
     CDS             complement(8157..8870)
                     /locus_tag="PLF85_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNE19284.1"
                     /translation="MADEVKPSEQFASPESVLAKFQTLDTLGLSAGQRPINGARFTGK
                     GPRVFFLGGVHGNETEGVAATAGFYDEYVAANKVAGLPCDLLFIPVLNPDGLLAFSRH
                     NGNAVDLNRNMPTKDWVQNAPGEKYYSGPAAGSEPETQLLLQILTDFQPEFILSLHSW
                     KPMFNVNGPAHGHAQKMHAALPYEITEDIGYPTPGSLGTYAGWERQIPTITFEIERGL
                     PLQQVWPTVRGAVLAAITG"
     gene            complement(8874..9803)
                     /locus_tag="PLF85_06180"
     CDS             complement(8874..9803)
                     /locus_tag="PLF85_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNE19285.1"
                     /translation="MALFDSLPSSNFALSGWVDGKKLMGKKLAELVRADVAREAAKLA
                     VKPGLAVILAGDDAASQIYVAGKEKAALEAGFTSRIVRLPATVTQEELVAQIHAFNND
                     ATIHGILVQLPLPKHLNEEQILQAIVAEKDADGFHYLNQGRLLAGLPTTLPCTPAGVA
                     LMLRELSRERSDYKLEGKHAVVVGRSNIVGKPMAQLLLSGLNMTVSMCHSRTRNLGDF
                     IRSADLIVSATGVRGVLDLASMKPGAIVVDVGMHRIDGKVTGDLDLKIAEEKAAFYTP
                     VPGGVGPMTIAMLLYNTLRNAGLLQANQLVARG"
     gene            complement(9876..>10274)
                     /locus_tag="PLF85_06185"
     CDS             complement(9876..>10274)
                     /locus_tag="PLF85_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19286.1"
                     /translation="PVMAIRNFIAACEEQLERAQEPFAVIVTDIANYSRLQNLHGDSH
                     ATAVRDFTRRTLREIMESQDFSTEVFHGHFVSVLRQKEAGDAWRLSRIMQKQAGRFYA
                     DEDLRPLFQHRIYARPHIQAIPFELLFKAV"
BASE COUNT         2145 a         2791 c         2980 g         2358 t
ORIGIN      
        1 agttgagtga ttttaccgcg ttgcccggca aagtgaattc ggttgaaccg ggaagaaagt
       61 ttcgccagaa tgccctgaac gaagaggcgc gcgccccagg tggtaaacgg ccccgcagct
      121 cgatgtcgcc gctcatcatg cgttttgacg acggcagagt cgtcgcgctg ggtagcccgg
      181 gtggcccgac gattgtcggc acagtggcgc tcaccgccgt acgcgtactc gcgggccagg
      241 agctgcagca ggcggtcaat gcaccgcgcg cgctgatgat gccgcacggc aaagcactgc
      301 tcgaacttcc gctacggcga aacaaggcgt tcctgcaggc gttgccgctt gcaggcatcg
      361 aagctgacct ggggcgaaag atcatttcgc tggggtcagt gcaggcagtc gaatgcactc
      421 ccacagcgca gaaagacagg gtaacagtag cggaaaaaac gcaaagcatg gtgtgcactg
      481 cggcgaatga cctgcgccgc gagggcctgg ggctcgtacc aaatatcagg gttgattaga
      541 tgataccgaa tcttgcagcc gccgatttct gggtgattgc cgcgtacttt gtgctcacct
      601 ttggtgtggg agttttcgcg ttgcgccgga ggcatgaaac caaagaggat tattttctct
      661 ccgggcgttc tttaccgtgg catctggccg ggctttctat ggtggcgacc acattcgccg
      721 ccgacactcc gctcgcggtc accggcatca cgattgaacg cggtatcgcc ggaaattttc
      781 tctggttgtc gcttattcct tctggtattc tgaccgcgat tttttatgca aggctctggc
      841 ggcgttcggg cgtcatgacc gacgccgaat ttgcgacgct gcgctattcg ggaaaacctg
      901 cgcgcttcct gcgccgcttc cgtgcgctct acctcgcggt tccggttaac ctgatcatca
      961 tggggtgggt gacgacgggc atggcgaaag tgatgcaggt actcttccag tttccgacct
     1021 ggcagactct cggtatgctc tatgcattta ccgtgctcta catcgtgctc tcgggcctgt
     1081 ggggtgttgt cattacggat gtgctgcagt ttttgatcgc gatcaccgca tgtatcgagc
     1141 ttgcggtgat tgccaccggc catgcaggcg ggctcgacca gctatggcag aatgcagaaa
     1201 agacactcag gccaggtgcc acagaaattt atccatggta ctatgccggg ccggtttatg
     1261 tcgttgtcgt ctggcttggc gcgcagtggt gggcgtcgtg gtaccccggc tttgagcctg
     1321 gcggcggtgg ttacgtcgcg cagcggttgc tttcgacacg caatgaaaac cacgctgttg
     1381 ccgctgccct gctttttaac gtactgcatt ttgtcgtgcg tccctggcct tggattatca
     1441 ctgcgctcgc ggcagctgtc gcgttgccgc agggtactga taaagagctc ttgtacccgc
     1501 aggcaattct cgcctggatg ccttcaggtt taaaggggct catggtcgcc gcgttccttg
     1561 cagcattcat gtcgacgatt gcgacgcatc ttaactgggg cgcgtcttac attctgaatg
     1621 acgtctttgc cggtacgaga ttcgcaccgc aaacccagcg cggcgaaatg ctgctttcgc
     1681 gcattaccat cattttcctg gccgttgcag ccgtgggagt ttcttttttg atggattcag
     1741 tgagtgcagg ttgggagctc atcctcagca tttctgccgg cacggggcct gtgtacctgt
     1801 tgcgctggta ctggaagcgc atcaacgcgc tcagcgaaat cagtgcaatg gccggtgcct
     1861 tcttgttctc gttgctgctc gcaggttttg acctgccgca ggagatcagg ctctgtctcg
     1921 ttgcgctttt caccagtatc ttctgggtgg cagtgacgct ggttacccgg cccgaaggca
     1981 cagaaaagct ggctgcattt gaaaaactgg tttcgccgcg ccggtggacg ctgcgcgagc
     2041 tcgttgtttt tgtcgccgca gtggccgtga tctatagcgc gttctttctg ataggcgagc
     2101 tgttgcgcaa gaattggtat gcagtctcgc taaatgcgct gggtcttgca gtagcgcttg
     2161 cagtgacgta tgccgccctg cgcaggcgtg tcaattgagc agtatacttt tgtgagtgct
     2221 ggcaaagtgg cggtaaacgc cgcccttttc gatcaggcaa cgccgccaga ggtcataacc
     2281 atcgtcgtgg aacaccagat cgggacgtgc gtcgagcaat acccagctat tggagcgcaa
     2341 ttcgtactcg agctgccccg tggaccagcc cgcatagccg cagaatcgcc ggtagcgaaa
     2401 tttcttttgc atcagcacag gtaacaagtc gtctgagctt tcaaaataga cgccgtcgat
     2461 aatcggttca ccggtggcga attcatccgc gccatgcagc agcgaacggt gttcaggatc
     2521 taccgggccg ccggcgaata gcggcttatg cctgcgggtc tcgcccggca gcggatgatt
     2581 cagcaccagc ccgagggagc ccctgctgtc gtggtcgacc agtaatacga cactacggtt
     2641 aaaattgccg tcgagtattg ctgaactcgc gatcaacagt ttgcctttca gtttatcgtc
     2701 catcaattat gaatagattt tagctgctca atcagcgata gctgcacata attactgagc
     2761 gcgcgcgtat cggctgtttc gttgccatat ttgcggacga tctgggtcgc aacgttgctg
     2821 atttcacctg agagaaaact ttgggtaatc tcgggtagct tgcgtgtcat tgaatagagt
     2881 cgctttgccc gctgcgcgat atcggtgtca accctttcgc tgagctgccg gcgtgtatcc
     2941 ccgagtttga tgttccagag ttctgcgtac ttaccgatca tttccatcgc gtttttttcc
     3001 ccgatcaggc gcgtttctgc catgttgagc gcacggtgcc agtcggtatc cgcactgttg
     3061 accgtcgtct ttggcggtga gtcttcaacc cgggcggctg ccggttgttc cgagccgacg
     3121 agtccaaaca gggtggccag ccagcgtagt agcggatacc ggtagagaaa tggcagctcg
     3181 gcgcgtacca ccgcaacaat ctcgcggcga accagttcga ggagcgaatg tacggggcga
     3241 aaaatgatgc ggtcctttcc ggcaaagaag tagagctcct gcaaattcgc actcataggg
     3301 tgccggctga taatgtggaa ggcgttaagt atcgcctgcg cattctgcag caaggttgaa
     3361 aataccggct catgcgcctt gacgtatttc tccacgtctt cagcaaagaa ctcgtcaaac
     3421 tccatcgacg aacgactttc attgcgtagc atcagtgcgt accacatttt gcgaaagcgc
     3481 gaaagcagtt cagcccgcac acgcttgcgt tcagattcaa aaaaaatgag cagagcctca
     3541 cggtgaataa agaattcttc ttcgttcatg cgaaagcgca gcagggcagg aagcagctcg
     3601 cgttcgtctg cctggaagac cgtgtattca gagagcatat cctgcacaac cagttcgatt
     3661 tccgcctggt tgagcagcga agccatgatt tcgagacccg cgctgccctt gacaactttt
     3721 tgaataatac cttcccggtt cagcagagca gggtaatctg ccatcatgtt gagcagcttt
     3781 tgcgagtttt cccgaaggcg atccttattg tttttttccg attcgagcca ggcttcgtat
     3841 cctttgaacg cctcgacaag tctggcggcc tgcagcaagg tagacatgcc gcgcttgtct
     3901 ttgtccattg tgaaataggc agccaggcgg tttgcgagat ttaccaggta caggggtact
     3961 tcagattcgt tgtgcgacag gatgcgcata atacgttcga cgttcgacgt cttctgcggc
     4021 atgatattct gcatgtcctt tacgaggtct tcggcaagcc ggttttgtgc cgaattggca
     4081 atgacattcc gcagcttcag caggcatatt tcgagcatca tgtcgacggt gttaccggtt
     4141 gcacataccg cctgcatcat gtcgccaaag cgaaagagga tcaggcgctt ttcttctttc
     4201 agccggtggt tcttgccgct ggaaatctca gaaacagcaa tatcgacgtg gttgttcggc
     4261 agcaaaaacc tgcggaccat gtcttcgtcc ggaaaaggca gtgccgcgtt ggaaagcatt
     4321 tgcccgtaat attcttcgag aaagagcgtg gcagggtcag tcagtcgaat gcgtaaaact
     4381 tcaccgccgt caaggtctgc atcgatgaac tttttttcgc gcagcggaaa agccgtctgc
     4441 gtaatcaggc ggcggacatt ggggagcgtc tttagttttg caatattctc gtcgctaccg
     4501 cgcgataaaa gaaaatccgt gaggcgcgcc gtggaaaaag agatggtatc attctgtgcc
     4561 gcataccggg cgtaagattc gagcatcaga gcctcgtctt ccataagcgg aataccgcgt
     4621 gcaaaatcac ggaattgctg ttcgtcgagg tcgatataga atggcttatg ctccgtggac
     4681 actgttttga ttatcggctc ttaatgggcg tattaattac ttatatgcag taggatatag
     4741 ttcctacata atgcgggcac gttggggaga tcaggccagc gtaacccacc gcgggtcgag
     4801 ggtataaatg cccggcattg cgaaatcgcc cagcgcagcg aggcgactta agcccagcgg
     4861 caatcccgca cgtgagaaga gcgactcgtg caccgtgggc agtatggcaa tttcgagcgg
     4921 tgacaaggca gacaacccca gtttttcccg gactgctgcc agcgcgtcgg tcaggccacc
     4981 tttttggtct gcgagaccaa gggtgtgtgc acgtttaccc gagtatacgc ggcccgaggc
     5041 caacggccgg attttgtcct gccccatacc ccggccctga gcaacatcgc gcaagaactg
     5101 ctcgtagatt tcgtgcatat tggcggtgag ctgttgcttc tgtaccggcg cgaaatcggc
     5161 aaatgccgaa aagaacggcg cagtattctt tttgggtgca cggtcgacgt tgacgcccca
     5221 cttggcgagc aggggtgata cattgaattt gccgccgact acaccgattg aaccggtaat
     5281 tgtcagcggt gtcgtgaata cactctcgcc agccgccgac agatagtagc ctcccgatgc
     5341 cgcgacgttg ccctgcgaaa caacaaccgg caatggctgc cgttcctttc ttgttttcgc
     5401 ggcacgcttt gtcttctttg cctctgccac cgggctacct aatttattgc cctcaagccg
     5461 gtgtgttgcc agcatccatt cgcgccagag cagctgcgaa acgagcgccg agccacccgg
     5521 gctgttgata cgcagcaggg ctgcgttgaa gtgcccgctg cgcagttcgt ctgctacact
     5581 ttcgtagtca tgccagttga ttgttccggg gcgcgggcga tggctttcga cgatattgcc
     5641 ttcggcaacg atcagcgcaa cacggtgggg ccgccggaag ggaagcagcc tgaaggcacg
     5701 cgcccggacc agtgttagcg cctgcgacag gtcgcgcatc gctgtttttt cggatgcctt
     5761 gatccaggca ccgtactcct gcgcagaggc cgtaccctgc aacaggccgg ctgccattgc
     5821 agcggtcgca gaaagcagtt gtggtctgtc ctttctgccc agcgacggag cgcggccgga
     5881 gatcgcagaa aagaactcgg cttcaaggtc tgccaccagc tcttctgtct gctgccttgc
     5941 tgcacgcgac atattgttgc gcgtaaaaat ttcggcagcg ctcttgaaat ctcccaccga
     6001 aagaaattgc ggccttacac ccagcttgtt cagcatcgag agcatgaaga gcgattcgct
     6061 ggtaaagggt gaaaggtcaa aggtggcagc ttcggccgcg tagataaatt cgcagccagt
     6121 agcaatgtag agcgatacgc ggtcgtcggc aatcagccag cacgacgtgc gaataccctg
     6181 ttcacgggcc gactcgagga gccgcttcag ctcccacgcc tgtgcccagc ccagattgtg
     6241 cgattcaata gtcagcgata tatgactcaa cttaagccgt ctggcagctg cagccgtaat
     6301 aaccgactgc agctcaaggc aaagcaggta gaaacgatca cggtcgggtt taaaaaaggc
     6361 ccagatgccg tgtgaaagcg gcgcttcgga gaactcgccg cgcaccgcca gctgtaatac
     6421 aaagggccgg tgcagtgccc gcagcctgaa ccaggcgtag acgatgaggc gtagaggttg
     6481 cagtacggcc agcaagagag cgcggagcat ggtttcaagt gcgctgcaag acagggcgaa
     6541 aagccgcaaa ataggtgacg gtgccgccag attctcctcg agtgtgctgt ccgaagggtc
     6601 gatagtttcc tgtggggggt gaccagccgc gccgatggca ctaaacaccc acctgagtca
     6661 ggaatttttg atgcgtggat ttattcttcc tttactagta accctggtgt gttgcgccag
     6721 caaagcgacc cgcgtgccgc cggaagccga aaaggcactt gcgcgcgtca cgcagaacgt
     6781 gcagaccttt cccgaaggcg ctgacaagca gccaccgcag ctcaaacggc tcgcttctgc
     6841 gggcgtaacc cttaatgcct ggtccaggga ttttgcccgc ggcagcgcca tcctgctaga
     6901 attcgagtca tccgataacc tcgcgcaggc ggagcttacg gtcaatgacc ggccgctggc
     6961 gctttcgcgc agcgagtcag gaatattcgc cctgtttgcc aattcacctg acagcaagct
     7021 aaaacagaac cgcgtggttc tgaagcgttc cggtgcagca caggcagaat ttgtcttacc
     7081 agttgtcagc aaggagtacc cggtagcggt ctcgaagatg acggtaaaca aattctctaa
     7141 ccagagcaag ccgatgagcc aggaaactat cgacgccatt gaagcaggac gaaagaagaa
     7201 gctgaaggca ctcgtctcgg gttcgggtaa cctgctcacg ggcgattttc gttacccgcg
     7261 ggatgagcac aagatcacct caccgttctt catcaagcgt gtctatgagc gcttcaggct
     7321 cgtgaagggt aagaaaaaga aactgcccgg taaaacctcg taccatggcg gcacagacct
     7381 caagggcctg aagggagctc ccatttttgc gatcgcggac ggtgtcgtga acctggcgga
     7441 gcacctgttt tacgaaggca acatcgtgat cctggatcac ggtaatggag tgatcagcgg
     7501 ctacatgcac cagagtgagc ttgtcgtcaa agaaggcgag aaggtcaagg caggacagct
     7561 gatcgggcgc agcggtgata caggaatggt taccgggcca cacctgcaca tctttttgct
     7621 tattcatggc gtgaaagttg acccgctgag cctgctggta ttgcctgtac ggcccggacg
     7681 ctgatggcaa gcggccatac tgaatttgtc gtcatacttc tgctggcggt tgtttttctt
     7741 gcggcaggag tttttatcgg cgttcgcatc agcagcctgc gcgccgaggc gaagtttgtt
     7801 tctatcctgc agcaaaagga agcggagtgg gggcagagtg ccgaagccga gcggcgccag
     7861 gcactcgcca aatcgcgcca ggtaatcggc ggttcgttca gtgagcaact tgccccttat
     7921 ctgccggact ttccgtttga cccgaccgag gcgcgcttta tcggcaaacc cgtggacttt
     7981 gtcgtctttc gcggacttgc tgccggggcg gtggaggaat tggtattcgt cgaagtgaaa
     8041 tcggggaaaa gccgtctcaa tcgcaacgaa agttccattc ggcatgccgt tgcggctggc
     8101 cgtgtcagct tctttgagta ccgcgtaccg gctggaatta ccagcgaacc gggcgattaa
     8161 cccgtgatgg cggcaagcac cgccccgcgc acggtcggcc agacctgctg cagcggcagg
     8221 ccgcgttcga tttcgaatgt aatcgtgggt atctgccttt cccagccggc gtaggtaccg
     8281 agtgacccag gtgtcgggta gccaatatct tcggttattt cataaggcag cgcggcatgc
     8341 atcttctgtg cgtgaccatg cgctggtcca ttcacgttga acatcggttt ccacgagtgg
     8401 agcgacagaa taaactctgg ctggaagtca gtgagaatct gtaacagcag ctgtgtttcg
     8461 ggttcagacc ccgcggctgg tccggagtag tatttttcgc ccggtgcatt ctgcacccag
     8521 tctttcgtcg gcatgttgcg gttgagatcg acagcgttgc cgttatgacg tgagaaggcc
     8581 agcaaaccat cgggattcag aaccggtatg aacagcaggt cgcacggcag accggctacc
     8641 ttgttagcgg cgacatattc atcgtagaag cctgcggttg ccgcaacacc ttcggtttcg
     8701 ttgccgtgca ctccgccgag aaaaaatacc cgtgggcctt tgccggtaaa ccgcgcgcca
     8761 ttgatcggac gctgacccgc cgacagcccc agtgtatcca gtgtctgaaa ttttgccagc
     8821 acagactcgg gtgaggcaaa ttgttctgat ggttttactt cgtctgccat ggtttatccc
     8881 cgcgcgacca gctggtttgc ctgcaaaaga cctgcgtttc gcagcgtgtt gtaaagaagc
     8941 atcgcaattg tcatcggtcc gacaccccct ggtaccggcg tatagaaagc cgctttttct
     9001 tcagcgatct tcagatcgag gtcgccggtt actttgccgt cgatgcggtg catgcccacg
     9061 tcaacaacga ttgcacccgg cttcatgcta gccaggtcaa gcacgccgcg tacgccggtg
     9121 gcgctgacga tcagatcagc agaacgtatg aaatccccga ggttgcgggt acgtgaatgg
     9181 cacatgctta ccgtcatgtt gaggcccgag agcagtaact gtgccatagg tttgcccacg
     9241 atattcgagc ggccaacaac taccgcgtgt ttgccttcca gtttgtagtc gctgcgctcg
     9301 cggcttaact cgcgcagcat cagtgctaca ccagctggtg tgcagggcag ggtagttggc
     9361 agaccggcaa gcagtcgccc ctggttgagg tagtgaaagc cgtccgcatc tttttcagcg
     9421 acgatagcct ggagtatctg ttcttcattc aggtgtttag gcagcggcaa ctgcacgaga
     9481 atgccgtgta tagtggcatc gttattgaaa gcgtgtatct gcgcgaccag ctcttcctgt
     9541 gtcacggtag caggcaggcg cacgatgcgc gaggtaaagc ctgcctcaag ggctgctttc
     9601 tctttgccgg caacataaat ctgcgatgca gcatcatcgc ccgcgagaat taccgcgagc
     9661 ccgggtttga cggcaagttt ggccgcttcg cgcgcgacat cggcgcgcac cagctcggcc
     9721 agtttttttc ccatgagttt cttgccatcg acccagcccg aaagggcaaa attgctggag
     9781 gggagtgaat caaaaagtgc cacggcacac gaaaccggaa ggttcgacag gcttacaatc
     9841 agaagaggaa tcgacctgtt gagggggcaa aaaaatcaga cggccttaaa cagtaactcg
     9901 aaaggtattg cctgtatatg cgggcgagcg tagattctgt gctggaacaa cgggcgcaga
     9961 tcttcgtctg cgtaaaacct gcctgcctgc ttttgcatga tgcgcgaaag ccgccaggca
    10021 tctccggcct ctttctgccg cagcacactc acgaagtggc catggaatac ctcggtggaa
    10081 aaatcctgcg attccatgat ttcgcgcagc gtgcggcgcg tgaaatcgcg cactgctgtc
    10141 gcgtgtgagt cgccgtgcag gttctgcagg cggctgtagt tggcaatgtc ggtcacgatc
    10201 accgcaaacg gttcctgcgc gcgttcgagc tgttcttcgc aggcggcgat gaaattgcgt
    10261 atcgccatga ccgg
//
LOCUS       DAOQJF010000084        10187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3440_length_10187_cov_5.081623, whole genome shotgun sequence.
ACCESSION   DAOQJF010000084 DAOQJF010000000
VERSION     DAOQJF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_10187_cov_5.081623"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PLF85_06190"
     CDS             <1..1269
                     /locus_tag="PLF85_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE19287.1"
                     /translation="MILVAPLSMAAEEASSTPAAEEEKKTYSLVQITERVRESSELVQ
                     AQKLKIESTEAQGRQATAWQNPSVSAELGRLRDPNNNAMTYDFRLTQPIYFPGKMGKQ
                     MEVYEREKEIQEMQAADLTTSLALEASYYAYAYKVAELKSRSINNRINRMSLMRTYMR
                     AQAYASPAKIIQRNIVANQLLALQKTLNTAMAETESSWQKLNIYLNEQDKVVVAAPWL
                     TELKEVDIVTFINHVREGNRRLKSGKLLLSRIEAEVSFQKRVPLPDVSVSAFYRREAL
                     EHPGANQFYGGAVTVPIPVLNANRSGVEAAEKRLEAARAETNYAQREVLQMARAVHAE
                     YTQKRKLLNDFNAEQIPLLEKQMLYADRELKLGRIDLLSYLELELQTYQAIQSFYDTQ
                     LELVKAITQMIYLMGDSAKYKGDLYVFQAN"
     gene            1250..4525
                     /locus_tag="PLF85_06195"
     CDS             1250..4525
                     /locus_tag="PLF85_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNE19288.1"
                     /translation="MFSRLIDWALNNRTVVIGIAVALCIAGLYSVTRLPVDAVPDITN
                     VQSMVITKTGALDPQKVEATVTYIIETELQGIPKVEEIRSISKYGLSLIVIVFEEDAN
                     IYLARQQISERLNQVQDSLPAGIVPELAPLSTGLGEVFFYVVEAKPDSELAKKPEKER
                     LIYLRTVQDYIIKRQLKGVKGVADVDTFGGYPKEIHINLDTRKLEANGLTIEQVSERL
                     NTLGENYGGGYIQREGKQIVIRTLGRVTDLDKIRKVPIKRSYFGAPIRLGEICTVTIH
                     HPQPLGGATYRGEEAVIGMPMMLAGANSRNVAAAIGDAFKDLDLPQDVQVRPLYSRDY
                     LVNATVKTVLKNLAEGAGFVVVVLLMILGNVRAAILVSLAIPISMLIAVIGMNYFHVS
                     GNLMSLGAIDFGLLVDGSVVMFENMIRRMEEHDWSLPLEGHRKRNMILESTLEVLRPI
                     ITALLIIMGVFIPLFALEGVEGKMFRPMAQTVLLAMAASLVLAIVLMPVLGYMFFKLK
                     KPKPGEHHDHTPFILKYILKGYEPLLNFALKNPKKIIAPTVALTVISIGLFFRMGGDF
                     IPQLNEGDMVINFSRPVQSSFDTVLAMQKKSDKIITSFPEVKEVYSRFGTPQSATDPA
                     GVFFSDTFVILEKDTSKWRKKNGRTISKDDLYKEIIEAIDADQSVGKQESSGTQPIEM
                     RFNEMLEGSRADVTMRIYGTDLDKLAEMTIQGQQILEKVKGVESVQSDPIIALSKSPV
                     LDIELNYDAISYYGLDLLDVNRVVEMAMSGHKIGNLYEGAYRYPIVTRLDPRLRNDVA
                     QVRNIPVGLDSGGSVPLYKVATINEREQVSTIARSQGRRYAALSINLGDIDVETFVKN
                     AQAEIKKQMQLPEFYEITWGGQFKNLQRAKAKLAIIVPVTLLGIFLLLIRYFGSYKQS
                     LLVFMGIPTAIVGGVLTLSVVGMNFSISAGVGFIALMGIAILNSLVLVSHYDEQLAKN
                     PDESVLRLVLEGTLDRFRPVVITTLVAGLGFLPMAINSGLGSEVQKPLATVVIGGLII
                     ATVLTALLLPALYTMIFGKNGNAAALTASPAPGSAFTQEQVSFAEDLEEDSGKKRKRG
                     KKN"
     gene            complement(4626..5357)
                     /locus_tag="PLF85_06200"
     CDS             complement(4626..5357)
                     /locus_tag="PLF85_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19289.1"
                     /translation="MPRASLSRLLFFFTACLVWPHNLAAETGHFRCRGTDKQTRKLVF
                     TCSGVEIRRKGVPVSAEIVYRTARGKVFAKEKINFRKDALAPDVLFQDLRDGRREMVE
                     REGNHFSVIIQDSAKAEPEFARLQHPGEDEQVLTMPGLPAYLATTMEQMPVESRLTFR
                     VLFPQERKMLKLRAILEANTRYQRREALRVRVQPQNFVYRWFSQPVFLTIQKKNGRLL
                     RYEGLHYVRNPENGHGTIVDLVFSW"
     gene            complement(5375..6346)
                     /gene="dusA"
                     /locus_tag="PLF85_06205"
     CDS             complement(5375..6346)
                     /gene="dusA"
                     /locus_tag="PLF85_06205"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNE19290.1"
                     /translation="MTAAVVPWEKVSIAPMMEITDRRFRYFMRLINPKVRLYTEMIVA
                     QAITRGNRDRLLRFDSTEHPVVLQLGGSDPQEMAAAAHIGEEFGYNGIDVNCGCPSDR
                     VGSGNFGACLMRDDEKVADIVARIVASVRIPVSVKCRIGITGEESEEKLLRFAQKIFA
                     AGACRLTVHARTATLGGLSPKENRSIPPLRYDWVYRLKSHLPQHEIEINGGIRSREEI
                     LTHLASTDGVMLGRLAHEDPFFFYAGTRADSLGARLHILEMMADYAEQNEDPGLTAAR
                     FFNNMLNLFHGLPGSRRIRRFLSEQGRSLSPRQAYQQLVADSALPHD"
     gene            complement(6347..8737)
                     /gene="lon"
                     /locus_tag="PLF85_06210"
     CDS             complement(6347..8737)
                     /gene="lon"
                     /locus_tag="PLF85_06210"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNE19291.1"
                     /translation="MAENKEPLIVPINQLLPPELFILPVRHRPVFPGMVTPLVIAEGR
                     LAEAIENVHKQNQYLGLVLQHDDTSSEVGPDQLYRVGTAAKILKRINLPEGGMHLLVN
                     SLERFRIQSFVHSTPQMLAKVEYQIEATQVRDKELIALMRSVLNQAKEMMTENPYFTE
                     EMKLTLINVEEPGKIADFVSSVLNLKREEFQEILETFDIRRRLERVLQFLARELDLVK
                     LQRKIQGKVNQGVEHNQREYYLREQLKAIKSELRGPETRDKDGDYYRQKIEAAKLPAE
                     ANERALDECGKLDYIEPSSHEYTVIRNYLDTILDLPWNIPAVDDIDLSYARQVLNRDH
                     FGLDDVKTRILEYLAVRSLKSDVKGSIICLVGPPGVGKTSLGKSIAAAMKKKFYRFSL
                     GGMRDEAEIKGHRRTYIGAMPGKLIQGLKSTKAKDMVLMLDEIDKLGRSYHGDPASAL
                     LEVLDPAQNSEFRDHFLDLPFDLSQITFITTANSLDSIPEPLLDRMEVIRLPGYILEE
                     KLQIAKKYLVGRAIADSGLTKKNAPQLSLTGLRFLIDGYAREAGVRRLEREIQKLYRK
                     AAAALVEKKPFAKTLTPKEIPKYLGKPVFTRADELTTLAVGSAIGLAYTAMGGATLTI
                     ESRKFPGRGKVKFTGQIGKVMNESAEIAVSFARSLENDDRFWGKHDIHIHVPDGATPK
                     DGPSAGITITTALLSLLRNQKIKSGYAMTGEIRLSGDVLPIGGLREKIVAARRVGIRN
                     VIFPYDNLRDYEELPGELRKGLKIFAVKHYSEIEQLLFSRAKKSNPVKKTKAGKRR"
     gene            8835..9818
                     /gene="phnD"
                     /locus_tag="PLF85_06215"
     CDS             8835..9818
                     /gene="phnD"
                     /locus_tag="PLF85_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE19292.1"
                     /translation="MKQRLLVFFFSLILASGCGGGDGVDISDLAAGRASEVYGQEDIK
                     ARGSLSENLPGEAINFGITPWDEPDKLREMYAPFVAYLSERMKINIRFMVAQEYQELL
                     NDLRRGIIHIAAFSPGSYADALDSGIESFADYVASTQNEGNSYYRGVIIAGKNYRDLK
                     ALKGESFAFVEKGSSSGYKFPLALLLAKGVDPYRHFSKVFYLGSHANVVDSVVSGKVA
                     AGATWDGYLERQLGKNPEKVTVLYRTEQIPYDALVVSRKKGRRFANQLRAELLRINKE
                     TTTKDGRKVLQKELGFPYSGYVVHPSGFYNVVRRTGNLVKQYKPPAETNVK"
     gene            9808..>10187
                     /locus_tag="PLF85_06220"
     CDS             9808..>10187
                     /locus_tag="PLF85_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HNE19293.1"
                     /translation="MLSKFISEKVFPYLQSLQEKRILPESVYRRIRENSLAGKINFII
                     LSSAVPMLMLISALFLFLYFQLSRQVDATLVQKMIGARNAYNYYERTTLVYAKMLAEN
                     PYIKKELIAESVNVGPILRVANQVA"
BASE COUNT         2363 a         2823 c         2732 g         2269 t
ORIGIN      
        1 atgattcttg tcgcgccact ctcgatggct gccgaagaag cgtcatcaac acccgccgca
       61 gaagaagaaa agaaaacata ctcgctggta caaatcaccg agcgagtacg cgaaagtagc
      121 gaactggtac aggcacaaaa actgaagatc gagagtacag aagcccaggg tcggcaggct
      181 actgcctggc agaatccatc ggtttcggcc gaacttggca gactgcgcga cccaaacaac
      241 aacgcgatga cctatgattt caggctcacg caaccgattt attttcccgg gaagatgggc
      301 aagcagatgg aagtctatga gcgggaaaaa gagatccagg aaatgcaggc cgcagacctg
      361 acaacttcat tggcgctcga ggcttcgtac tacgcctatg cgtacaaggt cgccgagctg
      421 aaatcccgat cgatcaataa ccggataaac cgtatgtcat taatgcgcac ttacatgcgt
      481 gcgcaggcat atgcttcacc tgcgaaaatt atacagagga atattgtcgc caaccagctt
      541 ctggcgctgc agaaaaccct caataccgcc atggcggaaa ccgaaagctc gtggcagaag
      601 ctcaacatct atctcaatga gcaggataaa gtagtcgttg ccgcaccttg gctgaccgag
      661 ctcaaagaag tcgatatcgt cacattcatc aatcatgtcc gcgaaggtaa tcggcgtctg
      721 aaatccggta aattgctgct ttcgcgcatt gaagccgaag tctctttcca gaagcgcgtt
      781 ccgcttcccg atgtcagcgt gagcgccttc taccgtcgcg aggcgctgga acatccgggt
      841 gccaaccagt tttacggcgg ggccgtcacg gtccctattc ccgtactgaa tgcaaaccgc
      901 tctggcgtcg aagcagcgga gaagcgcctc gaagcggcgc gcgctgaaac caactatgca
      961 cagcgtgaag tactgcagat ggcacgcgcg gtgcatgcag aatacacgca gaagcgcaag
     1021 ttactgaacg actttaacgc tgagcagatc cctctactgg aaaagcagat gttgtatgcc
     1081 gaccgggagc tcaaactggg tcgtatcgac ctgctgtctt atctggagct cgagctccag
     1141 acttaccagg caatacaatc cttctacgac acccagctgg aacttgtaaa ggcaatcacg
     1201 cagatgattt acctgatggg cgacagcgca aaatataaag gagacttgta tgttttccag
     1261 gctaattgac tgggccctga ataaccgaac ggttgtcatc ggtattgcgg tagctctctg
     1321 catcgccggg ctctattctg tcacacgact accggtcgat gcagtgccgg atatcaccaa
     1381 cgtgcaatcc atggtcatta ccaagaccgg tgcactcgat ccgcagaaag tggaggctac
     1441 ggttacctat attattgaaa ccgagttgca gggtattcct aaggtcgaag aaatccgctc
     1501 catatcaaaa tacggcctat cgttaattgt tatcgtcttc gaagaagatg caaacatcta
     1561 cctcgcgcgg cagcagatca gcgaacgcct gaaccaggtg caggacagcc tgcccgcggg
     1621 tatcgtaccc gagctggcgc cactttcgac cggtctgggc gaggtcttct tttatgtagt
     1681 tgaagcaaaa cctgactcgg aactcgccaa aaaaccggaa aaagagcggc tcatctactt
     1741 acggacggta caagactata ttattaagcg ccagctcaag ggggtcaaag gcgtcgctga
     1801 cgtagatacc tttggcggct accctaaaga aatccacatc aacctcgata cccgcaagct
     1861 agaagcgaat ggtctgacga ttgaacaggt ttcggagcgt ctgaatactc tcggtgagaa
     1921 ttatggcggc ggttacatac agcgcgaagg aaaacagatt gtcatccgca cgctcggccg
     1981 agtaaccgat ctcgacaaaa tacgcaaagt gcctattaag cgttcatatt tcggggcacc
     2041 gattcgcctt ggtgaaatct gcacggtaac cattcaccac ccacaacccc tcggcggcgc
     2101 aacgtaccgt ggcgaagagg ccgtcatcgg tatgccgatg atgctcgcag gagccaatag
     2161 ccgaaacgtg gcggcagcaa tcggcgatgc attcaaagat cttgatctgc cgcaggatgt
     2221 tcaggttcgt cctctctatt cgcgcgacta cctggtcaac gcgaccgtca aaacagtcct
     2281 gaagaacctc gctgagggag ccgggttcgt ggtagttgtg ttgctcatga tcttgggcaa
     2341 tgtgcgtgcc gcaattctcg tctcgctcgc tattcccatc tcgatgctca tcgccgtgat
     2401 aggaatgaac tactttcacg tatcgggtaa cctgatgagc ctcggcgcta tcgacttcgg
     2461 tctgctggtc gacggttcgg ttgttatgtt tgagaacatg atcaggcgca tggaagagca
     2521 tgactggtca ttgccgctcg aagggcaccg taaacggaac atgattctcg aatcaacgct
     2581 cgaagttctg cgccccatta tcacagccct gctcatcatc atgggtgtct ttataccact
     2641 tttcgcactc gaaggtgttg aaggtaaaat gtttcgaccg atggcacaga ccgtcctgct
     2701 ggctatggcg gcgagtctgg tgctggccat cgtcctgatg cccgttctgg gctacatgtt
     2761 tttcaagctc aagaaaccaa agcccgggga acaccacgac cacacgcctt tcattctcaa
     2821 atatatcctg aaaggttacg agccactact caacttcgcg ctgaaaaatc ccaaaaaaat
     2881 tatcgctccc actgtagcgc tgaccgtgat ctctattggg ttattcttcc gtatgggcgg
     2941 agattttata ccgcagctaa atgaaggcga tatggtgatc aacttcagtc gtccggtaca
     3001 gtcgagcttt gacaccgtgc tcgccatgca gaaaaagtca gacaagataa ttacctcatt
     3061 cccagaggtt aaggaagtct acagccgttt cggcacaccg cagtcggcga cagacccggc
     3121 cggtgtattc ttttccgaca cctttgtaat cctggaaaaa gatacttcca aatggcgtaa
     3181 gaaaaacggc aggacgatta gcaaagatga cctctacaaa gagattattg aagctatcga
     3241 cgccgaccaa tctgtgggca aacaagagtc ttccggcacg cagccaattg aaatgcgctt
     3301 caacgaaatg ctcgagggtt cgcgcgctga tgtgactatg cgtatctacg gtaccgacct
     3361 ggacaagctc gccgaaatga cgatccaggg tcagcaaatt cttgaaaagg tgaagggtgt
     3421 cgagagcgtt cagtctgacc caattatagc cctttcgaaa agccccgtgc ttgatatcga
     3481 gctcaactac gacgcgatat cgtactacgg cctcgacctg ctcgatgtca accgcgtcgt
     3541 cgaaatggcg atgagcggcc acaaaatcgg taacctctac gagggtgcat accgctaccc
     3601 cattgttacg cgccttgacc cccggctgcg caacgacgta gcgcaggtac gtaacatacc
     3661 agtgggactc gattccggcg gcagtgtgcc actctataaa gtagcaacga tcaatgagcg
     3721 cgagcaggtt tccacgattg cgcgcagcca gggccgccgc tacgcggccc tttcgatcaa
     3781 cctcggcgac atcgacgtgg aaacgtttgt aaagaatgca caagccgaaa tcaagaaaca
     3841 gatgcagctg cccgagttct acgaaatcac ctggggagga cagttcaaaa acctgcagcg
     3901 cgcgaaggcc aagctggcga ttatcgtacc cgtcacgctc ctgggtatat ttttgcttct
     3961 catacgctac ttcggaagct acaagcaatc tttgctggta ttcatgggca ttcctactgc
     4021 aattgtcggc ggggtgctaa cgctgagtgt cgtcggcatg aacttcagta tttctgccgg
     4081 cgtcggattc attgccctga tgggtatagc gatcctgaac agcctcgtac tggtgagtca
     4141 ctacgacgaa cagctggcaa aaaacccgga cgagtccgtc ctgcgcctcg tgctcgaggg
     4201 tacactcgac cgcttcagac ctgttgtgat cacaacactt gttgcgggcc tgggtttcct
     4261 gccgatggcg atcaactcgg gtctcgggtc tgaagtgcag aagccgctcg caacagtggt
     4321 tatcggcgga ttgatcattg cgacggtact gacggcgcta ttactaccgg cactctacac
     4381 gatgattttc ggcaaaaatg gaaacgcagc cgctctgaca gcttcccctg cacctggttc
     4441 agcgttcaca caggaacagg taagttttgc ggaagatctg gaagaagatt ccggaaaaaa
     4501 gaggaaacgc ggcaaaaaga actaacaggc tcctgaaaaa ggagccaaaa gcaggttacg
     4561 gaaaccgcgc aaggctgcag acgccgcggg agtttttccg gaacctgcta acaggaactg
     4621 ctcagctacc aactaaacac gaggtcgacg attgtgccat gaccgttttc gggattgcgc
     4681 acgtagtgca gtccctcgta acgcagtagc cgaccgtttt ttttctggat tgtgagaaag
     4741 accggctgcg aaaaccagcg gtagacgaaa ttctgaggtt gtacgcgcac acgcagcgct
     4801 tcccggcgct ggtacctggt atttgcctcg aggatagcgc gcagcttcag catcttgcgc
     4861 tcctgcggaa agaggacccg aaaggtgagc cggctctcga ccggcatctg ctccattgtt
     4921 gtcgcgaggt atgccggcaa tcccggcatc gtcaatacct gctcgtcttc acccggatgc
     4981 tgaaggcgcg caaattcggg ttcagccttc gcggaatcct ggatgattac cgagaagtgg
     5041 ttaccttcgc gttcgaccat ttcgcgccgg ccatcgcgca gatcctggaa cagcacatcg
     5101 ggcgcaagcg cgtccttgcg gaagttgatt ttttccttcg cgaaaacttt tcccctggcc
     5161 gtccggtaga cgatctcggc cgaaaccggg actcccttcc gccgtatttc tacaccgcta
     5221 caggtgaata ccagcttgcg cgtctgttta tctgtacccc ggcagcgaaa gtgcccggtt
     5281 tcggccgcaa ggttatgcgg ccaaacgagg caggccgtaa aaaaaaacag cagccgtgaa
     5341 agcgaggcgc gcggcacaac ccaaaagacc gccgctagtc gtgcggcaaa gccgaatcag
     5401 ccacgagttg ctggtacgcc tggcgcgggc tgagcgagcg tccctgttca ctcaggaacc
     5461 gcctgatgcg ccgcgatccg ggcagtccat gaaaaaggtt gagcatgttg ttgaaaaaac
     5521 gcgccgccgt cagaccgggg tcttcattct gctcagcgta atcggccatc atctcaagga
     5581 tatgcaacct agcgcccagc gagtctgctc gtgtgcctgc gtaaaaaaag aacgggtctt
     5641 cgtgcgccag cctgcccagc atgacgccgt cggtgctggc caggtgggta agaatttctt
     5701 ctctgctcct gatgccgcca ttgatttcaa tttcgtgctg cggtaagtgg gatttcaggc
     5761 gatagaccca gtcgtagcgc agcggcggta tactccggtt ctctttgggt gaaagaccgc
     5821 caagggttgc tgtgcgcgca tgaactgtca gccggcatgc acctgcggcg aatattttct
     5881 gcgcgaagcg gagcagtttt tcttctgatt cttcacccgt aataccaatg cggcacttta
     5941 cgctcaccgg tatgcgcacc gaagcaacaa tgcgcgcgac aatgtctgcc accttttcgt
     6001 cgtcacgcat caggcaggcg ccaaaattgc cgctacccac ccgatcgctc gggcagccgc
     6061 agttgacatc aatgccgttg tagccaaatt cttcgccgat gtgcgctgcc gcagccattt
     6121 cctgtgggtc gctgccaccg agctgcagca ccaccggatg ctcggtgcta tcgaagcgca
     6181 gtaaacgatc cctgttaccc cgggtgattg cctgcgccac gatcatctcg gtatagagcc
     6241 tcacctttgg attgatgagg cgcatgaaat acctgaacct gcggtcggtg atctccatca
     6301 tcggcgcgat ggagactttt tcccagggaa cgaccgcagc ggtcatctag cggcgtttcc
     6361 cggccttggt tttttttacc ggatttgact tttttgccct ggagaaaagc agctgctcga
     6421 tttcgctgta gtgcttcacg gcaaaaatct tcaggccttt cctgagctcc cccggaagtt
     6481 cttcataatc ccgcaggttg tcgtagggaa aaatcacatt gcgaatgccc acacgccggg
     6541 cagccacgat cttttcgcgc agcccgccga tcggcagcac gtcgcccgac agcctgatct
     6601 cgccggtcat cgcatatccc gattttatct tctggttgcg caacagcgac agcagcgccg
     6661 tggtgatcgt gatacccgcc gatgggccat ccttgggcgt tgccccgtca ggcacgtgta
     6721 tgtggatatc gtgtttgccc cagaaccggt cgtcattttc gagcgaacgg gcaaacgaaa
     6781 ccgcgatctc ggcgctttcg ttcatcacct tgccgatctg cccggtgaac ttcaccttgc
     6841 cgcgaccggg aaacttgcgc gactctatgg tcagagttgc gccgcccatc gcagtataag
     6901 ccaggccaat ggctgagcct acagcaaggg tggtcagttc atcggcgcgc gtgaagacag
     6961 gtttacccag gtattttgga atttctttcg gagtcagagt tttggcaaac ggtttcttct
     7021 ctaccagcgc cgcagcagcc ttacgataca acttctgtat ctcgcgttcc agccggcgta
     7081 cgcctgcctc gcgcgcataa ccgtcgatca ggaaacgcaa cccggtgagc gaaagttgcg
     7141 gtgcattttt tttcgtgaga cctgaatcgg caatcgcccg gccaaccagg tatttttttg
     7201 cgatctgcag cttctcttcg agaatgtaac ccggcaggcg gatcacttcc atgcggtcga
     7261 gcagcggctc gggtatcgaa tcgagcgaat tcgccgtcgt gatgaaggta atctgcgaca
     7321 ggtcaaacgg caggtcgaga aagtggtcgc ggaactcaga attctgcgcg gggtcgagca
     7381 cctcgagcag ggctgaggcc gggtcaccgt ggtaagaacg gcccaacttg tcgatttcgt
     7441 cgagcatcag caccatgtct ttggccttgg tagatttcag tccctgtatc agcttgcccg
     7501 gcatcgcacc gatgtaggta cggcggtgcc ccttgatctc tgcttcatcg cgcatgccgc
     7561 cgagcgaaaa gcggtagaac ttcttcttca tcgcggcggc aatcgactta ccgagcgaag
     7621 ttttaccaac acccggaggt cccacgaggc agatgatcga acccttgaca tcggatttca
     7681 ggctgcgcac tgcaaggtat tcgaggatac gcgttttcac atcgtcgagg ccgaagtgat
     7741 cgcggttaag aacctgcctc gcgtaagaca gatcgatatc gtccactgct ggtatgttcc
     7801 agggcaggtc gagtatcgta tcgaggtagt tgcggatgac ggtgtattcg tgcgagctcg
     7861 gttcaatata atcgagtttg ccgcattcat cgagcgcgcg ctcgttggct tcggcgggca
     7921 gcttggccgc ctcaattttt tggcggtagt aatcgccgtc tttatcgcgc gtttcagggc
     7981 cacgcagctc gctcttgatg gccttcagct gctcgcgcag gtagtattcg cgctggttat
     8041 gctcgactcc ctggtttact tttccctgga tcttgcgctg tagcttcacg aggtcaagct
     8101 cgcgcgccag gaattgcagc acccgttcga gccgtcgcct gatatcgaag gtctcgagaa
     8161 tttcctggaa ctcttcgcgt ttgaggttga gtacactcga gacaaagtcg gcaattttgc
     8221 ccggttcttc gacgttgatc agcgtcagct tcatctcttc ggtaaaatac gggttttccg
     8281 tcatcatctc tttggcctgg ttcagcacgc tgcgcatcag cgcgatgagc tctttgtcgc
     8341 gcacttgcgt ggcttcaatc tggtactcga ccttcgcaag catctgcggc gtggaatgca
     8401 caaaagactg gatcctgaaa cgttcaagcg agttcaccag caggtgcata ccgccttcgg
     8461 gcaggttgat gcgtttgaga atcttggccg ccgtgcccac ccggtagagc tggtcgggac
     8521 ctacttcgga gctcgtatcg tcatgctgca gtacaaggcc gaggtactgg ttctgcttgt
     8581 ggacgttctc tatggcttct gccagcctgc cctcggcaat caccagcgga gtcaccatgc
     8641 caggaaacac cggccggtgc cgcaccggca gaatgaacag ttcaggcggc agaagctggt
     8701 ttatcgggac aatgagcggt tctttgtttt cggccacggc ctatatttta acaaaaaaca
     8761 aggcaaaaat ccatttttga cgggggaaaa acacatttac tgacgatccg ctgcttgcag
     8821 cgtagcaatt caacatgaaa cagcgactgc tggttttctt ttttagcctg atactggcca
     8881 gcggctgcgg gggcggcgac ggggtcgata tttctgacct cgcggcggga cgtgccagcg
     8941 aggtctacgg gcaggaagat atcaaggccc gaggtagcct gagcgaaaac ctgcccggcg
     9001 aggcgatcaa cttcggcatc acgccatggg acgaacccga caaactgcgt gaaatgtatg
     9061 caccgttcgt ggcgtacctc tccgagcgga tgaagatcaa tatccgcttc atggtggccc
     9121 aggaatacca ggagctgttg aatgacctgc gccgcggcat catacacatc gccgcttttt
     9181 cacccggttc gtacgccgat gcgctagact cgggcatcga aagttttgcc gattacgttg
     9241 cctcgacgca gaacgaggga aactcctact accgcggggt gatcatcgcg ggcaaaaatt
     9301 accgcgacct gaaggcactc aagggcgagt cgtttgcctt tgtcgaaaaa ggctcgagtt
     9361 ctggctataa gtttccgctc gcgctactgc tcgccaaggg cgttgacccc taccgccact
     9421 tttccaaggt cttctacctg ggttcacatg cgaacgtggt cgattcggtg gtctcgggta
     9481 aagtcgccgc gggtgccacc tgggatggct acctggagcg gcagctgggt aagaacccgg
     9541 aaaaagtcac ggtactctac cgcaccgagc agatccctta cgatgccctc gtggtctcca
     9601 ggaaaaaagg caggcgcttt gcaaaccagc tgcgcgcgga gttgctgcgc atcaataagg
     9661 agacgacaac aaaagacggc cgcaaagttc tgcaaaaaga actcggcttt ccgtattcag
     9721 gatacgtggt gcacccctca ggtttttata atgtagtgcg cagaactggt aatctcgtaa
     9781 aacaatacaa gccacccgca gagacgaatg ttaagtaagt tcattagcga gaaagtgttt
     9841 ccctacctgc aatccctgca ggaaaaacgg attctgcccg aatcggtcta ccggcgtatc
     9901 cgcgagaact ccctcgccgg aaaaattaac ttcatcatac tctcatcggc ggtaccgatg
     9961 ctcatgctga tttcggcgct ttttctcttt ctttacttcc agctatcacg ccaggtcgat
    10021 gccacgcttg tacagaaaat gatcggggcg cgtaacgcct acaactacta cgagcgcacg
    10081 acgctcgtct acgccaagat gctcgcagaa aacccgtaca tcaagaaaga gctgattgcc
    10141 gagagcgtga acgtaggccc gattctgcgc gtggcgaacc aggttgc
//
LOCUS       DAOQJF010000085        10138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3462_length_10138_cov_5.458395, whole genome shotgun sequence.
ACCESSION   DAOQJF010000085 DAOQJF010000000
VERSION     DAOQJF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3462_length_10138_cov_5.458395"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..429
                     /locus_tag="PLF85_06225"
     CDS             55..429
                     /locus_tag="PLF85_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNE19294.1"
                     /translation="MFSQGSLSVSLLVLSNIFMTLAWYGHLQLHKSERFSHLPLYSVV
                     LLSWLIALAEYTFQVPANRIGSENYGGPFNLFELKVIQEVVSLLVFTFFAVYVFRTEQ
                     LRWNYLIGFGFLVLAVYFIFKK"
     gene            complement(499..1575)
                     /locus_tag="PLF85_06230"
     CDS             complement(499..1575)
                     /locus_tag="PLF85_06230"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19295.1"
                     /translation="MSPASVHRLVAAAATGAMLVGLVACASIPVQGDRHKVMTSDDWA
                     LTVEHLPSLNAAAKKKYPVLICHGLGANREYFKAKDEDSLVRNLQRAGYDVWLMDLRG
                     RVEAAETGYWFGKHTYTYDIDAYIKHDLDAAIKFVLQQTGAQKVNYIGHSMGGIVMYA
                     RLGTQNEGRVANLVTIASPMSFLPYNDWTFKLYRMRGGMAILPVLPIRPGAFMASFFP
                     EMLYAPFANAFYNPENTDAKVKAQLLRKSINNISKNEIKQFIYMTDNGGLYSADGKTN
                     YRSELKNVIVPAYLVAGRRDELADPAVVRDVYERLGSKDKTMEIFSRADGYADDYGHT
                     DLIFGKQAHKEVHPKIIEWLNKRN"
     gene            complement(1572..2108)
                     /locus_tag="PLF85_06235"
     CDS             complement(1572..2108)
                     /locus_tag="PLF85_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19296.1"
                     /translation="MRIFSAAMVLLLVSHVSAIAPNQLGNDFNDGIQQSNLKLGIGWS
                     QENHDSLRAHLLQGQFEFFMSDRLSLRGNAGVPLSTAVSETKYYPFMLGGAFHVLPRY
                     WIDFYFGADAGFVHIATATLPATWSTRVTPVVGATVYFWGAFFLEAEAGYNILQYAKD
                     VAIDMSAPTYRVRTGFYL"
     gene            complement(2183..2776)
                     /locus_tag="PLF85_06240"
     CDS             complement(2183..2776)
                     /locus_tag="PLF85_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNE19297.1"
                     /translation="MNGKFGKRVFTPQSMVEILTDIVRAAPTMPGTFTSGRISPAFRE
                     RILLAVTSVNRCRYCQWVHSDLAAANGISADQIASLLGSSTQSVPEEEKVALLYALHY
                     AETNRNPSPLHRQQLVDTYGSETAHDIENWIHLIFFSNLSGNTFDAFLSRLRGEPNPE
                     GNAIFEAMFAVLSAPVLLSLAALSKNSVNPLVSLEHN"
     gene            complement(2778..3350)
                     /locus_tag="PLF85_06245"
     CDS             complement(2778..3350)
                     /locus_tag="PLF85_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE19298.1"
                     /translation="MPKIVNHREYRSELLEKSAGTFSRHNYADVSMRDIAKDIGVSTG
                     TLYHYFPSKEELFCALFLHTAQSSAAEVVDAMCNLTTFEERLDRLFDYFLGRCDRMRR
                     QFLFSADMLRNALPHKAQKLLNRWASELERRLSGVLGLDSETGLAVFLFLAGSLYAGQ
                     VLPASRDLEPGFNAMKQMLLQQHKTKVKGK"
     gene            complement(3503..4939)
                     /locus_tag="PLF85_06250"
     CDS             complement(3503..4939)
                     /locus_tag="PLF85_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE19299.1"
                     /translation="MNPVIWRSILLALLVLLSACVPSLPRREHTAMELPKEYLQAESS
                     PEAQMLADKVWSQFFTEPRLVGLIDTAVKNNQELAMLEQEISVANNEIMARHGEYQPK
                     VSGQAAGGVEKAERFSSADANGPTRFAHAGLVMNWEVDIWKKLRNATKAAYLRYLAGI
                     EGRRYVVTNLVAEISYTYYELMSLDNQLRAVQDYVEVVAKVKNMVDLQRQAGRTTSLG
                     VKRFEAEVAKNRAREYDLKQRIAIAENRLNLLLGRFPQPIERDPSRYLETAFSEIQTT
                     VPVKLLENRPDIRQASLNLEARKLDVEVARARFYPSLTIDATVGYEAFNSEHFDGTPV
                     SLAYGLVGGLTAPLLNRKAIEASYRSANDLQIQAVFNYEFTLLQAFTEVTNQIIKVKN
                     LRSRYEMKQQELKALRDSIQISNVLFKAGRIEYIDVLLTQRDYLEAEISLLEVKQTQL
                     EATIGLYKAMGGGWRGQHEQSREPHWQP"
     gene            complement(4936..8091)
                     /locus_tag="PLF85_06255"
     CDS             complement(4936..8091)
                     /locus_tag="PLF85_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE19300.1"
                     /translation="MFAKFLRRPVLALVLSIVVVFMGLLSIKTLAVSQFPEIAPPRVT
                     VTLAFPGASAQVLVQSSITTLEQAINGVPGMRYMISSSTSSGDAIIQVFFEPGTNPND
                     ALVQVKTRVDQMMYRVPPLVRLEGILVQPVQPSMLLYVNLYSTAPNATEKFLYNYANA
                     FLLPELKRIPGVGQARILGSRQYAMRVWLDPDRMRAYNVSTTEVMKAIESQSIIARPG
                     RLGQSSGKKAQSLEYTLTYEGWYSDPQQYENIIVRAKDGGEVMYLKDIAKVELDSEFY
                     NIYSDVDKHPAAAIMFKQTEGSNAREVIEQIKNRLAELKPQFPPQMDYKLGYDVSRFI
                     DASIEKVMHTLFEAFVLVAIVVFVFLGDWRSTLIPAIAVPVSLIGAFTFMKALGLTIN
                     LITLFAMVLAIGVVVDDAIVVVEAIHAKMAEEGLGVYGAVKAVLGEISGAVIAITLLM
                     TSVFVPVSFLPGPVGVFYRQFAITMATSIVLSGVVALTLTPVLSAMILRPHDHGKQHH
                     GPVGKLLERFNTYFDRVTARYVELMRRFSSNRVLTVMILLAFSLGTVIFSRMVPSGFV
                     PGEDQGMIYAIIQTPPGSTIEKTNDVAQKLQEEALKIEGVDSVASLAGYEILTEGEGS
                     NAGTCLISLKDWSERKNSVHTIMHELEQRTKEFGAVIEFFEPPAVPGFGAAGGVSFRL
                     LDKTNSGDYAAFDKVHVQFMEELRKRKELTGLFSFYSAKFPQLLVKLDRKLAMQKGVN
                     IGEAMENLSILIGSTYEQGFVRFNQFFKVYAQAAPEFRRLPSDILKLYTQNEKGEMVP
                     YSAFMTLEQTQGANEITRYNAYTSSVINALPQKGYTTGDAIRAIQEAAQTLPVGFDVA
                     WEGLTYDEARRGNEAVLIFLIVIAFVYLVLAAQYESFILPFSVILSLPPGIFGTFFLL
                     KLMGLANDIYAQIGMIMLIGLLGKNAVLIVEFARQRQEAGLSVFEAAIEGAKARFRPI
                     LMTSFAFIAGLLPLVVATGPGAIANHTIGACALGGMLFGTVFGVVLVPGLYYIFATLA
                     EGKQLVFNEDEMPLTEKRRRK"
     gene            complement(8099..9220)
                     /locus_tag="PLF85_06260"
     CDS             complement(8099..9220)
                     /locus_tag="PLF85_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE19301.1"
                     /translation="MRYENKTRGRLLPVKSKPAIMLLIVLIAAGANCKRHDRQQGEAL
                     SAAKPWRQEVTVRQAYVAQVKAIQHIELRAFEKGYLTHIYVDEGQVIRKGQKMFQIMP
                     TLVKATYEKARAEYEATTIEFENTEKLFNENVVSATELALVRARLKKNKAAMDLAKSH
                     LDLSTVTAPFSGIMDKFHVRLGSLVDEGALLTTLSDISKLWIYFNVTEKDYLNFVGEK
                     NKKGNPIKVRFVMANGKEFRHDGVADTIEGEFDSDTGTIPFRATFPNPDRLLRHGETG
                     NIIIREKLENALVIPQKSTFEVLDKRYVYVVNEAGVLNATEIKVSHEVPHLFVVESGI
                     SEKDTILLEGLGKAKNGQVVATKLEVPEEILKSFELAAH"
     gene            complement(9756..>10138)
                     /locus_tag="PLF85_06265"
     CDS             complement(9756..>10138)
                     /locus_tag="PLF85_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-transporting ATPase"
                     /protein_id="HNE19302.1"
                     /translation="THRAFFRQLFLLLGFPFRVLYMRLSNFTVGVRGFVIAPLLRALA
                     GFFGRHPILFNFVSNPLVNIGIASEILLCYVFFYTDLRKIYFFEPVPWHVYLFAFHGL
                     VLIVLFEEIKKYFRRRGHALEFLG"
BASE COUNT         2349 a         2753 c         2678 g         2358 t
ORIGIN      
        1 acctattgcc accgaattaa ttcggtggca atagggtcgc acggcaggtg cctcatgttt
       61 tcacagggtt cgttgtctgt ttcactgctt gttctttcga acatcttcat gacgctggca
      121 tggtacgggc acctgcagct gcacaaaagc gaacgttttt cgcacctgcc gctctatagc
      181 gtggtgctac tgagctggtt gattgcgctg gccgaataca ccttccaggt accggccaac
      241 cgcatcggca gcgaaaacta cggtggcccg ttcaacctgt ttgagctcaa ggtgatacaa
      301 gaggtcgtct cgctgctcgt cttcaccttc tttgctgtgt atgtattcag aaccgaacag
      361 ctgcgctgga actacctaat aggtttcggt tttcttgtcc tggcggtgta tttcatattc
      421 aagaagtagc cagcatcggt gcttcgactt cgctcagcaa ccgacgaccg gcccctgagc
      481 gcagtcgaag ggcggtcgtc aatttcgttt attcaaccac tcaatgattt tcgggtgtac
      541 ttctttgtgc gcctgtttgc cgaaaatgag gtcggtatga ccgtagtcgt cggcataacc
      601 atcggcgcgc gagaagattt ccatcgtctt gtcttttgat ccgagacgct cgtagacatc
      661 gcgcaccacg gccgggtctg ccagttcatc gcggcggccg gctacgagat aagccggcac
      721 tatgacattt tttagttcgc tgcggtagtt ggtcttgccg tcggcagaat agaggccgcc
      781 gttatcggtc atatagatga actgtttaat ttcgttcttg ctgatgttgt tgatcgactt
      841 gcgcaagagc tgcgccttga cctttgcgtc ggtgttctca gggttgtaga aagcgttggc
      901 aaacggtgca tagagcattt cgggaaagaa gctcgccata aaggcacccg ggcgaatcgg
      961 cagtacaggc agaatcgcca taccgccgcg catgcggtag agtttgaacg tccagtcgtt
     1021 gtaaggcaaa aagctcatcg gtgaggcgat agtgaccagg ttcgcaaccc tgccctcgtt
     1081 ctgcgtaccg agccgcgcgt acatgacaat gccgcccatg ctgtgaccga tgtagttaac
     1141 cttttgtgct cccgtctgct gcagcacaaa ctttatcgcg gcatcgaggt catgtttgat
     1201 gtaggcgtcg atgtcgtagg tgtaggtgtg tttgccaaac cagtagccag tttctgccgc
     1261 ctcaacacga ccacgcaggt ccatcagcca gacgtcataa cccgcgcgct gcaggttgcg
     1321 cacgagcgag tcttcatctt tcgccttgaa gtattcgcgg ttcgcaccaa gcccatggca
     1381 gatcagcacc gggtacttct tcttcgcggc agcattgagc gatggcaagt gctcaaccgt
     1441 cagcgcccag tcgtcggaag tcatcacctt gtggcgatca ccctgcaccg gtatactggc
     1501 gcaggccacc agacccacca gcatagcgcc ggtggccgct gccgctacca ggcggtgaac
     1561 actggctgga ctcatagata aaagcccgtg cgcacccggt aagtgggagc gctcatatca
     1621 atcgcgacat ctttcgcgta ctggagtata ttgtaacccg cttcggcctc gaggaaaaat
     1681 gcaccccaga aatagaccgt tgcccccacc acgggtgtga cgcgcgtcga ccaggtcgcc
     1741 ggcagagtcg ccgtcgcaat gtgcacaaaa cccgcgtcgg cgccgaagta gaaatcgatc
     1801 cagtagcgcg gcagaacgtg gaaagctccc ccgagcataa aaggatagta cttagtttca
     1861 gagaccgcag tactgagcgg aacgccggca ttgccgcgca gcgaaaggcg atcgctcata
     1921 aaaaattcga actgcccctg caaaaggtgt gcgcgcaggc tgtcgtggtt ttcctgcgac
     1981 cagccgatgc ccagcttcag gttactctgc tgtatgccgt cattgaagtc attgcccagc
     2041 tggttgggag ctatcgcgct gacgtgactg accagcaaaa gaaccattgc cgcagagaaa
     2101 attcgcatgc ggcaaagggt cgatgttgac ggcattgtca acttcttgcc aaattagctc
     2161 cgacaggctc agcctgttag agctaattat gttccaaact taccagcggg ttcacagagt
     2221 tcttgctgag cgccgccagc gaaagcagca ccggggcaga cagcacggcg aacattgcct
     2281 cgaagatggc attgccttcg gggttcggct cgccgcgcag gcgcgagaga aatgcatcga
     2341 acgtgttacc cgacaggttc gagaagaaga tcaggtgaat ccagttttcg atgtcatgcg
     2401 cagtttcgct gccataggta tcgacgagtt gctgccggtg cagcggcgaa ggattgcggt
     2461 tggtttcggc ataatgcagc gcgtagagca aggcgacctt ctcttcttcg ggtacactct
     2521 gtgtagagga gcccagcagc gacgcaatct ggtcagccga tatgccgttg gctgccgcaa
     2581 ggtctgaatg cacccactgg cagtaacggc agcggttaac cgaggtcacg gcgagcaaaa
     2641 tgcgctcgcg gaatgcaggc gagatgcgac cgctcgtgaa tgtaccgggc atcgtcggcg
     2701 ccgcgcgcac gatgtcggtc agaatctcta ccattgattg gggtgtgaac acccttttgc
     2761 caaattttcc gttcatgtta ttttcccttt acttttgttt tatgttgctg taagagcatc
     2821 tgcttcatcg cgttgaagcc cggttcaaga tcgcgcgagg ccggcagcac ctgccccgcg
     2881 taaagactgc ccgcaagaaa cagaaagacc gcaaggccgg tctcagagtc gagaccgagt
     2941 acgcccgaga ggcggcgctc gagctcactc gcccagcgat tgagcagctt ctgcgccttg
     3001 tgaggcagcg cgtttcgcag catatcggcc gaaaacagaa actgccggcg cattcggtcg
     3061 cagcgaccga gaaaatagtc gaacagccgg tcgagccgct cttcaaacgt agtcaggttg
     3121 cacattgcat cgaccacctc ggcggcggag ctctgtgcgg tatgcagaaa caacgcacaa
     3181 aaaagctcct ctttcgaggg aaagtaatgg tagagtgtgc ccgtcgagac gccgatgtct
     3241 ttcgcgatgt cgcgcatcga cacgtcggcg tagttgtggc gtgaaaatgt accggccgac
     3301 ttttcaagca attcgctgcg gtactcgcgg tggttgacta tctttggcat tttatatcga
     3361 acgattgata tataataata tcatcagagg gtggcaattt tttttgctta cgggatgcct
     3421 tagaggttgc cgcagcgact ggtaaccgcg cttcactctc tgagctttag cccggagagt
     3481 gaagcgctac cgcaaaaaga cctcagggtt gccaatgggg ttcgcgcgac tgttcatgct
     3541 gaccgcgcca gccaccaccc atagctttgt agaggccaat cgtcgcttcg agctgggtct
     3601 gcttgacctc aagcaggctg atttctgcct ccaggtaatc acgctgcgtt aacagcacgt
     3661 ctatgtactc gattcggccg gctttaaaca gcacattgga tatctgtata gaatccctga
     3721 gcgccttgag ttcctgctgc ttcatctcgt aacgcgagcg cagatttttg accttgatga
     3781 tctggttcgt gacctcggtg aatgcctgta gcagcgtaaa ttcgtaattg aacaccgcct
     3841 gtatctgcag atcgttcgct gaacgatagc ttgcttctat tgcttttctg ttcagcagtg
     3901 gcgcggttaa accgcccaca agcccatacg caagcgatac cggcgtgccg tcaaaatgtt
     3961 cggaattaaa agcttcgtaa ccgactgtcg catctatcgt cagcgatggg taaaacctcg
     4021 cgcgcgcaac ttcgacgtcc agctttctcg cttcgaggtt gaggcttgcc tgccgtatgt
     4081 ccggacggtt ttccagcagc ttcacgggca ccgtagtctg aatttccgag aaagcagttt
     4141 cgagatagcg cgaagggtct cgttcgatcg gctgggggaa tcgccctaac agcagattga
     4201 ggcggttctc tgcaatcgcg atgcgctgtt tcagatcata ttcacgggcg cggtttttcg
     4261 caacttctgc ctcgaacctt ttgacgccga gtgatgtggt gcggcctgcc tggcgctgca
     4321 ggtcgaccat attttttact ttggcgacca cttcaacgta atcctgcact gccctcagct
     4381 ggttatccag tgacatcagc tcgtaatagg tgtacgagat ttctgctacc agattcgtaa
     4441 cgacatagcg tcgcccttcg attcctgcaa ggtaacgcag gtatgcggcc ttggtcgcat
     4501 tacggagttt cttccagata tcgacttccc agttcatgac gaggccggcg tgcgcaaagc
     4561 gcgtcggtcc attcgcatcg gcggagctaa atctttctgc tttctccact ccccctgctg
     4621 cctgtcccga aacctttggc tggtattcgc cgtgacgcgc cataatttcg ttattcgcta
     4681 cgctaatctc ctgctccagc attgccagtt cctggttgtt tttcacagca gtatcgatca
     4741 accctacaag acggggttct gtaaaaaatt gtgaccagac cttatcggcc agcatctgtg
     4801 cctcaggcga actctctgct tgcaggtatt ctttaggcaa ttccatcgcg gtatgctcgc
     4861 gacgcggcaa agatggcacg caggcactga gcagcacaag cagggcaaga agaatactgc
     4921 gccagattac cggattcatt tgcgcctcct tttttccgta agcggcattt catcttcatt
     4981 aaaaacgagt tgttttcctt cggcgagtgt cgcgaaaata tagtacaggc caggcaccag
     5041 taccacgccg aaaaccgtac cgaagagcat tccaccgagg gcacaggcgc caatcgtgtg
     5101 attggcaata gctcccggcc ctgtggccac cacaagaggc agcagtcctg caataaatgc
     5161 gaaggaggtc atgagaatcg gacggaaccg cgcttttgcg ccttcaatcg cagcctcaaa
     5221 aacgctaagt cctgcctctt gccgttgccg ggcaaattcg acaataagaa cggcattctt
     5281 gcccaaaagc ccaatcagca taatcatacc aatttgggcg tagatgtcgt ttgccagccc
     5341 catgagcttc agcaggaaaa aagtcccgaa aatacccggc ggcaatgaca gaatcaccga
     5401 gaagggaaga atgaaactct cgtactgggc agcaagtacc aggtaaacaa aggcaatcac
     5461 aatgaggaag atcagcacag cctcatttcc acgccgggct tcgtcatacg tgagaccttc
     5521 ccacgcaacg tcgaagccga ccggcagtgt ttgtgctgct tcctggatgg cacgtatggc
     5581 atcgcctgtc gtgtatccct tttgaggcag cgcattgatc accgatgatg tataggcgtt
     5641 gtagcgggtt atctcgttag ctccctgcgt ctgctcgagt gtcatgaacg ccgaatacgg
     5701 taccatctcc cctttttcat tctgcgtata gagcttgaga atatccgagg gtaagcggcg
     5761 aaactccggg gcagcctgcg cataaacttt gaagaactgg ttgaacctga caaacccctg
     5821 ctcgtaggta ctaccgatca ggatactcag gttctccatg gcctcgccga tattaacccc
     5881 cttctgcatc gcgagcttgc gatcgagttt cacgagtagt tgcggaaact tggccgaata
     5941 gaaggagaag agcccggtga gttcttttcg cttacgcagt tcttccatga attgcacgtg
     6001 tactttgtcg aacgcagcat agtctccact gttggtctta tccagcaatc gaaacgagac
     6061 accgcccgca gcgccaaaac caggcactgc aggcggctca aagaattcaa tgaccgctcc
     6121 aaattctttg gtcctttgct cgagttcgtg cataatagtg tggaccgagt tcttgcgctc
     6181 tgaccagtct ttcaggctga tgaggcaggt gcccgcattg gatccctcac cttcggtgag
     6241 aatttcgtag cccgcgagcg aagcaacaga atcgacacct tcaattttca gcgcttcttc
     6301 ctgcagtttt tgtgcaacat cgttggtctt ttcaatggtc gaaccaggtg gggtctggat
     6361 aattgcatag atcatcccct ggtcttcccc gggcacaaaa cctgatggca ccatgcgact
     6421 gaaaatcaca gtcccaaggg aaaatgccaa caggatcatt accgtgagca cacggtttga
     6481 agaaaagcgg cgcatgagtt cgacgtagcg ggccgtaacc ctatcaaagt acgtgttgaa
     6541 ccgctccagg agtttgccga ccggcccatg gtgctgtttt ccgtgatcat gcggccgcag
     6601 gatcatggcg gagagcacag gcgtcagcgt gagcgcaact acaccggaaa gcacgatcga
     6661 tgtcgccata gtaatggcaa attgccggta gaaaactccg acgggccctg gcaaaaatga
     6721 aacggggaca aacaccgatg tcatgagcaa agtaatcgca atcaccgcac cgcttatctc
     6781 acccaagacg gctttgaccg cgccataaac cccgagaccc tcctctgcca ttttggcgtg
     6841 aatagcttca accacaacaa tggcatcatc gaccacaaca ccgatcgcca gcaccatagc
     6901 aaagagcgta atcaggttaa tagttaatcc cagagccttc atgaaggtga atgccccaat
     6961 cagcgacaca ggtacggcaa tggccggaat gagcgtcgat cgccaatcac ccaggaacac
     7021 gaaaacgacg attgcaacga ggacaaatgc ttcaaacaat gtatgcatga ccttctctat
     7081 cgatgcgtcg ataaatcggg aaacgtcata acccaatttg taatccatct gcgggggaaa
     7141 ctgtggcttc aactcggcta gcctgttctt gatctgctcg attacctcgc gcgcgttgct
     7201 tccctcagtt tgcttaaaca tgatggcagc agcagggtgc ttatcgacgt cagagtatat
     7261 gttatagaac tcgctatcaa gctcgacctt cgcaatatct ttcaggtaca ttacctcacc
     7321 gccatctttg gcgcgcacga taatattttc gtactgctgc ggatcactgt accagccttc
     7381 gtaagtgagc gtgtattcga gcgactgtgc ttttttgccg gagctctgcc ctagccggcc
     7441 tggccgggcg atgatgctct ggctttcgat tgccttcata acttcggttg ttgagacatt
     7501 ataggcgcgc atacggtcag gatcgagcca gacgcgcatg gcgtactggc gcgagccgag
     7561 aatgcgcgcc tggccgacac cgggtatgcg cttgagctca ggcaaaagaa aagcgttagc
     7621 gtagttatag agaaacttct ccgtggcatt aggggcagta ctgtaaagat tgacataaag
     7681 cagcatactc ggctgcaccg gctgcaccag tatgccttcg agtctcacaa gcggtggaac
     7741 gcgatacatc atctggtcta ccctggtctt cacctgcacc agcgcatcat tgggattcgt
     7801 gcctggctcg aaaaacacct ggataatcgc gtcacctgaa ctggtcgacg acgaaatcat
     7861 gtaacgcata ccgggaacgc cattgatggc ctgctcaagc gttgtaatcg atgactggac
     7921 aagtacctgc gcactggcgc ccgggaatgc cagggtgaca gtaacgcgcg gtggcgcaat
     7981 ttcaggaaat tgcgaaaccg cgagcgtctt gatcgaaagc agccccatga agaccacgac
     8041 aatggagagc accagcgcaa ggactggccg ccgtaaaaat ttggcaaaca tggtaacctc
     8101 agtgagccgc tagctcaaaa ctcttgagga tctcttccgg aacctcaagc ttcgtcgcga
     8161 caacctgccc gttctttgct ttgcccaggc cttccagcag gatggtgtcc ttttccgaga
     8221 taccgctctc aacaacaaac aggtggggta cttcgtgaga cactttgatc tccgtcgcat
     8281 tcagcacgcc ggcttcgttg accacgtaga cataccgctt atcgagcact tcgaaggtcg
     8341 acttttgcgg aatcacgaga gcattttcca gtttttcccg gatgataatg ttacctgttt
     8401 ccccatggcg caacaggcga tcaggattcg ggaaagtagc acgaaacgga atggtaccgg
     8461 tatcgctgtc gaactctcct tcgattgtat cggcgacccc atcgtgcctg aattcctttc
     8521 cgtttgccat aacaaagcgg actttaatcg ggttcccttt cttgtttttt tcgccaacaa
     8581 agttcagata atctttttct gtgacattaa agtaaatcca gagcttcgag atgtccgaga
     8641 gagtggtgag caaggcaccc tcgtcgacca ggctgccaag ccgcacgtga aatttatcca
     8701 ttattccact gaacggagca gtcaccgttg agagatcgag gtgactcttg gccaggtcca
     8761 ttgccgcttt gtttttcttg aggcgggccc gcacgagggc cagctcggtg gctgagacta
     8821 cgttttcgtt aaaaagcttt tccgtattct caaattcgat tgtcgtcgcc tcatattctg
     8881 cccgcgcttt ttcatatgta gccttgacca gtgtgggcat aatctggaac attttttgcc
     8941 ccttcctgat cacctgccct tcatcgacat aaatatgcgt taggtagcct ttctcgaagg
     9001 cgcgcagctc gatgtgctga atggctttca cctgggcaac ataggcctgt cggacggtaa
     9061 cctcttgtcg ccatggtttc gcggcgctga gtgcttcacc ctgctgccgg tcgtgtcgtt
     9121 tgcagttggc ccccgcagcg atcaagacga tgagcagcat aattgccggc ttacttttca
     9181 cgggcaataa gcgcccgcga gttttattct cgtatctcat aaaatccccc tgtaaccggg
     9241 cttagtgcgc cggcttattt ctgcatggct gtatgcagct cttacctgtt attctgaatt
     9301 gctgctaagt caatgcagcg ggggaccacg agattcgggg gttaggtaga agaggaaata
     9361 acgttcatat tgaatggatg ccgagaggaa tgcagagtga acaacgcgcg gtgccgtttg
     9421 tgccgggcca cagtcaacca ccacaccata ggcatgctca gcctcgttcc cttcgcttgc
     9481 ctcagcacag aaggagagat tcacagcggg ctggtggtct tgggatgtga agattacagc
     9541 cttgccggca aaagacggcg aggcctgggc aaacgcagga agaacccacg cgaaagtgag
     9601 tagcagcgca agcccttgag ccgtgagaag caaacgacgg ttaaccgatc gaactcccat
     9661 ctgctataag tataataaga tgtttaatac atgacaattt tttcgtgtgt ttagctttag
     9721 ggaccccagg tcaggtggcc gcggggcaga acaagctatc ccaggaactc gagtgcgtgg
     9781 ccgcgccggc ggaagtactt cttgatctct tcgaacagga ctatcagcac cagcccgtga
     9841 aaagcgaaca ggtaaacatg ccagggcacc ggttcaaaga agtagatttt gcgcaggtcg
     9901 gtatagaaaa atacatagca gagcagaatc tcactcgcaa taccgatatt aacgagcggg
     9961 ttcgagacaa agttgaaaag aatcgggtgc cggccgaaaa agccggcgag cgcccgcagc
    10021 agcggggcga tgacgaaccc acgcacgccc acagtaaaat tcgacagccg catgtagagc
    10081 acgcgaaacg ggaaacccag cagcagaaac agctgccgaa aaaacgcccg gtgcgtat
//
LOCUS       DAOQJF010000086         9954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3564_length_9954_cov_4.844429, whole genome shotgun sequence.
ACCESSION   DAOQJF010000086 DAOQJF010000000
VERSION     DAOQJF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3564_length_9954_cov_4.844429"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLF85_06270"
     CDS             <1..783
                     /locus_tag="PLF85_06270"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNE19303.1"
                     /translation="ATAIAKLEEQKWGSFGCDIVIESTGRFTDAHQARVHIDKGGAKR
                     VIISAPATNEDATFVLGVNEKNFDKAKHTVISNASCTTNCLAPVAKVLVENFGIKKGL
                     MTTIHSYTNDQQILDLPHKDLRRARAAALNMIPTSTGAAKALSLVIPDLKGKLDGISI
                     RVPTPDVSVVDLTVETEKPTTKEAVNAALKAAAEGPLKGILGYTDEPLVSNDFKGNPL
                     SSIVDGQMTAVIQDNMVRVLTWYDNEWGYSTRVIDLAAMIGK"
     gene            complement(840..2354)
                     /gene="pap"
                     /locus_tag="PLF85_06275"
     CDS             complement(840..2354)
                     /gene="pap"
                     /locus_tag="PLF85_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNE19304.1"
                     /translation="MRLRQNPYRYRMMMTDVLATMTGAAEIEKAEYKEREEKIRIRLL
                     ELQKMLATSGKAVLILMNGVEGSGKGETTKKLLEWIDARGVETTAFWTPAGEESEYPP
                     HWRFWNKLPGHGRIGIFFGSWYTAPIIERAFKQISRNKYDEYLEDIRKLEDMLTAEGV
                     ILMKTWMYLSPDAQKNILKDAEKKSATKWRVSKLTRKFAKKRKRFHKVDLEVLNATHT
                     GHAPWTLVDARFPRYREIFVGETLIKKLEEALIEKRGTPQYAAVWSPARSNYLTNLDS
                     TLKLTDEEYKEQFAALSAHISRLSDELSVSKKSLLLVFEGPDAAGKGGAIRRVIYGID
                     PRHYRVNGVAAPTDEEKSHPYMWRFWRRIPQHGQVCIFDRSWYGRVLVERIEKFCTDA
                     EWQRAYGEMRDFEHELTQAGTTIRKFYLSITSEEQLTRFKDRQLTPYKQYKLTEEDWR
                     NRDKWHAYEAAANDMFENTSTPFAPWVLVEANDKNHARIKVMKTVVEALEQALG"
     gene            2431..4302
                     /locus_tag="PLF85_06280"
     CDS             2431..4302
                     /locus_tag="PLF85_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNE19305.1"
                     /translation="MQPGNQQALSLHAYLARFPWRSDETQGRRPMDYLWKFSLPLSPE
                     ELWPFLIDTSTFNKLLGLPLMTYEEKNGRLFGKSVNAGVLSEWEEVPWEWEYLRGLNN
                     ARIYSRGFATYVRSRYILEQTGQGTDLYVYFGWVPRSLMGRLLLKVGMPRLRSDYEKA
                     FAKVISIIKDRSDFESRQRALFLAAQHEASEPAGTERLRILSDKAVKAGADSHLLRRL
                     ADFLVTAPEEEIARVRLKSVSRRLEIPLDAMVRTALYAAHAGILTLSWDVTCPHCRGV
                     RKSAESLGDVPAEETCEACDITFSTEDISNLEVVFHVNPEIRRAEKKFYCAAEPATKR
                     HILVQRRVGPGAALDLETSLAAGEYRVRLDGEKRYLPVSVGGGTERRIVIAENSSPLK
                     AAEQPVFVFPNRGAVERTLIIERKDEDNDALRPAEVFGVQLFRDLFGKETLAPGLKIE
                     LGRQNILFTDIVGSTNLYLSRGDGEAFTSVRLHFQKTYEVVRAFNGAVVKTIGDSAMV
                     AFTDSLQCLKAAVALQKSFTGNPETGNVLLRISIHSGPCLAVNLNSGVDYFGNTVNFA
                     AKLQQLAGEREIAYSRDFAQDPRISSYLRELGLQPEIRTFRPSWTDQTYDVPVLKIA"
     gene            4364..4972
                     /locus_tag="PLF85_06285"
     CDS             4364..4972
                     /locus_tag="PLF85_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19306.1"
                     /translation="MRFSLIISCAATLVAVACKSTGGTTSGDSGLPPLVANQPIKADN
                     TVSAYPGDAEVQAMREAFFARPVEAEEVFRVFVTSDGYMRKQIAHTETVSVKEDTGGD
                     QSIADEFRKFDMVNSLQEGQIRVELYPTTGKFYRVRQSKPSIMKETDKIMSDDITRWQ
                     FVFAKNEIQPKDFRISYQVMLRKKITREQALKILSEQNKKKK"
     gene            4978..5319
                     /locus_tag="PLF85_06290"
     CDS             4978..5319
                     /locus_tag="PLF85_06290"
                     /inference="COORDINATES: protein motif:HMM:NF040082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19307.1"
                     /translation="MSANEKKEIIIRVGLDENAIPESITWEAPGASKGEESADAFMLS
                     LFSGGEKTTLGIDLWTKEMLVGDMNIFFYQAFKKMADVLERATQDKDAAQLVRDFSRD
                     FGKQVKLLKDE"
     gene            5316..6653
                     /locus_tag="PLF85_06295"
     CDS             5316..6653
                     /locus_tag="PLF85_06295"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNE19308.1"
                     /translation="MTLFLASLAVAALLFGMPLFIGLGVLGTLYQFGNAESLGAESIA
                     DLSRQLTVVFSDPLSKLTGDQSALFLTIPYFTLAGYLIAHSQFAARVVNLYLVVMRRV
                     GALGAVGLGVICVLIAALFTPLTGASGVTIVAIGGILFPILTGAGYTERRALGLITAS
                     GSIGLLFSPSLPVILFGIMSENRADINELYRAGILPGILLIVALTVVVIFFTLGRTQR
                     ASDLLEQQLRAAQFSGSDAIKLVFELAAIPLLYVLMHSRFMIITELGVFFLLYYFLLE
                     VVVFRELGMKKLLGVFEEAIAMVGSIILIIFFAMILTNALLYDGVPGKLFGFISQYIH
                     SPLTFLMLLNIFLLIVGALMDIFSAIIVIAPLIIPIAESYQIPMLHLGILFLTNLEIG
                     YLTPPVGLNLFLSSLRFQKPMADVYRSIVPFLIALVVAQLIITYVPWLSLWWK"
     gene            complement(6657..7868)
                     /locus_tag="PLF85_06300"
     CDS             complement(6657..7868)
                     /locus_tag="PLF85_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNE19309.1"
                     /translation="MKKSDEKKGLSTRAIHAGHDNSADPHKPIVTPIYQTASYAFDEV
                     EGLWAFYQKRSDRLAEYARYGTPTQRALEETLCALENAEDCVVTSSGMNAIVSAIFGV
                     ANAGDHMVSLREGYRGTFKMFDQHLARFGISTSYADLTTAGVKTACTEKTRVIFIEFP
                     TNPYLRLVDLAEITAFARSRGITTIVDATFASPMNINPLEHGVDLVVHSLTKFISGHN
                     DAIAGAVLGSKELIEKVRSVRGLFGSNADAHLCFLVSRGIKSFAVRMREHNRSTLEIA
                     QWLEKHPKVTRVWYPMLESHPDHALAKSYLRGGGGVVSFQVKGKDSSQEAGLAASTAL
                     LNSLKIPAIAASLGGVESLIHQPSVMSYHDMDAAARLAAGIPDGLIRYAVGLEDTSDL
                     IADLERGLARL"
     gene            complement(7872..9194)
                     /gene="thrC"
                     /locus_tag="PLF85_06305"
     CDS             complement(7872..9194)
                     /gene="thrC"
                     /locus_tag="PLF85_06305"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNE19310.1"
                     /translation="MKAWFQSINPQNEQRYALDEILYRCPQTGDLLEVRHDMAALKKK
                     SAQEWRDLFESRWRSQNRVDASGIWGKREWVLPELPAEHVVTLGEGATQLFSGSELSR
                     RFNHDELYVKLCGNSHTGSFKDLGMTVLVSQVNHMIKKGASIRAIACASTGDTSAALS
                     AYAAYAGILSVILLPKNKISLAQLMQPIANGSLVLALETDFDGCMEIVQQLTTEKEIY
                     LANSMNSLRIEGQKTVGIEVAQQLGWQLPDWFIIPGGNLGNVSALGAGLKMLYELGLV
                     NSLPRIAVAQAEAANPLYLSYKKNFAEFSPITAGETQASAIRIGNPVSVKKAISTLRE
                     FGGVVEQASEQELADAAALGDRAGLLVDPHTGVALAALTKLKKGGVIKANESVAVIST
                     AHGLKFADFKVAYHAPEAKSEHKNLPQDLKPTLGAVKDAVARFLEKQR"
     gene            9299..9940
                     /locus_tag="PLF85_06310"
     CDS             9299..9940
                     /locus_tag="PLF85_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNE19311.1"
                     /translation="MNHRQMILYAVLCAVFIVTLPFKPYDLSFLPKVLPILLLVWLVN
                     AHANFRGRWLLLFALVCSGAGDVILEFRYGGAFIAGLVSFLVAHLFYIRLMVLTPSRE
                     LNRYIITLPVLVPPALVAWLLWPRLGALQVPVLVYVTVISTMLYTALTRVSFNRTLVA
                     GAAAFVVSDAVLAINKFYALFGAAPYTIMVTYYLAQLLLVRGFLAEARGTRAN"
BASE COUNT         2029 a         2701 c         2822 g         2402 t
ORIGIN      
        1 gcaacggcga ttgcgaagct cgaagagcag aaatggggtt cttttggctg cgacatagta
       61 attgaatcta ccggccgttt caccgatgcc caccaggcgc gcgtgcacat cgataagggc
      121 ggcgcaaagc gcgtcattat ctcggctcct gcaacgaatg aagacgccac tttcgtactc
      181 ggagtgaacg agaagaactt tgacaaggca aagcacacgg tgatctcgaa cgcttcgtgt
      241 accaccaatt gcctcgctcc ggtggcgaaa gtcctcgttg aaaactttgg catcaagaaa
      301 ggtctgatga ccacaattca cagctatacc aacgaccagc agattctcga cctgccacac
      361 aaagacctgc gccgcgcacg cgctgcagct ctgaacatga tcccgacatc gactggcgcg
      421 gcaaaagcac tttcactggt tattcccgat ctgaaaggta agcttgacgg tatctcgatt
      481 cgtgttccca ccccggatgt ttcggtagta gacttaacgg ttgaaacgga aaaacccaca
      541 acaaaagaag cggtcaacgc agctctcaaa gccgcagcag agggcccact gaagggtatt
      601 ctcggctata cagacgagcc actggtttcg aatgacttca aaggcaatcc cctgtcgtcg
      661 attgtcgacg gccagatgac tgcggtaata caggacaaca tggttcgcgt gctgacctgg
      721 tacgataacg agtggggcta ctcaacccgc gttattgacc tggctgccat gattggcaag
      781 taagaaaggg caaaagccga aagggaaatc cgtcgcgcag gcgacggatt ttttttgccc
      841 taacccagcg cttgttcgag agcttcaacc acggtcttca ttaccttgat gcgcgcgtgg
      901 ttcttgtcgt tggcttcgac caggacccac ggcgcaaacg gtgtggaagt attttcaaac
      961 atatcgttgg cggccgcttc gtaggcatgc catttatccc ggttgcgcca gtcctcttct
     1021 gtgagtttgt actgcttgta aggtgtcagc tgccggtctt tgaagcgggt gagctgttct
     1081 tctgaagtta tcgatagata gaacttgcga atcgttgtcc ctgcttgcgt gagttcgtgt
     1141 tcaaaatcgc gcatctcgcc gtacgcccgc tgccattcag cgtcggtgca gaatttctca
     1201 atgcgctcca cgagtacgcg gccataccac gagcggtcaa agatacagac ctggccgtgt
     1261 tggggtatgc ggcgccagaa gcgccacatg tacgggtgtg acttttcttc atcggtgggc
     1321 gccgcgacgc cgttcacgcg gtagtgcctc gggtcgatgc cgtaaatcac gcggcgtatt
     1381 gcgccacctt tgcccgcggc gtccgggcct tcaaagacca ggagcaggct tttcttgctt
     1441 accgagagtt cgtcgctgag gcgtgatata tgcgctgaga gtgctgcgaa ttgctctttg
     1501 tattcttcgt ctgtgagttt cagcgtcgag tcgaggttcg tcaggtagtt ggagcgcgcg
     1561 ggtgaccaga cggcggcgta ttgcggggtg ccgcgctttt caatcagcgc ctcttcgagt
     1621 tttttgatca gggtctctcc gacaaaaatt tcgcggtagc gcgggaagcg cgcgtcgacc
     1681 agcgtccagg gggcatgccc cgtatgtgtg gcattcagca cttcgagatc gaccttgtgg
     1741 aaacgtttgc gttttttggc gaacttacgg gtaagtttgg atacccgcca cttggtagct
     1801 gatttttttt ctgcatcttt cagtatgttt ttttgcgcgt cgggtgaaag gtacatccac
     1861 gtcttcatga gtataacacc ttctgccgtg agcatgtctt cgagcttgcg tatgtcctca
     1921 aggtactcat cgtacttgtt gcggctgatc tgtttaaagg cacgttcgat aatgggggca
     1981 gtgtaccaag agccgaaaaa gatgccgatg cggccgtgac cgggcagctt gttccagaac
     2041 cgccagtgcg ggggatactc ggattcttcg cctgcgggcg tccagaaggc ggtggtttcg
     2101 acgccgcgtg cgtcgatcca ttcgagcagc ttcttggtcg tctcgccttt tcccgaacct
     2161 tcgaccccgt tcatgagtat gagcactgct ttgcccgagg ttgccagcat cttctgcagc
     2221 tcaagcaggc gaatacggat tttttcttca cgctctttgt attcggcctt ttcaatttcg
     2281 gccgcaccgg tcatagttgc cagcacatcg gtcatcatca tgcggtaccg gtacgggttt
     2341 tgtcgcagac gcacagcaag tttttcccgg ccgccggctc tggcagacaa aaccgtggca
     2401 gatgcggcat aattgaagta aattccacgc atgcaaccgg gtaaccagca agcgctttca
     2461 ctccacgcat atcttgcgcg ctttccgtgg cgcagcgatg aaacgcaggg ccgccggccg
     2521 atggactatc tctggaagtt ttcgctaccg ctgtcccccg aagaactctg gccgtttctc
     2581 attgatacct ccaccttcaa caagctgctt ggtctgccgc tcatgaccta tgaagagaaa
     2641 aacggccggc ttttcggtaa gagcgtcaat gcaggagttc tttcggaatg ggaagaggtg
     2701 ccgtgggaat gggaatacct caggggtctc aacaacgcgc gcatctattc gcgaggtttt
     2761 gccacctacg tgcggtcgcg ctacattctt gaacagacag ggcagggaac cgacctgtac
     2821 gtctactttg gctgggtgcc gcgcagcctg atgggccgac tgctgctcaa ggtgggcatg
     2881 cctcgcctca gatctgacta cgaaaaggcg ttcgcaaaag ttatttctat aatcaaagac
     2941 cggagtgatt ttgaatcacg ccagcgtgcg ctctttttgg cggctcagca tgaggcgtca
     3001 gaacctgcgg gaaccgagcg cctgcgtatt ctcagcgaca aggcagttaa ggccggcgcc
     3061 gacagccatc ttctgcgccg cctcgccgat tttctggtta cggcacccga agaagaaatt
     3121 gcgcgcgtca ggctgaagtc ggtaagccgt cgcctcgaaa tacccctcga cgcgatggta
     3181 cgtactgcgc tctatgcggc gcatgccggc atactgacgc tttcgtggga tgtcacctgt
     3241 cctcactgcc gcggcgtacg taaatcggcg gaatcgctcg gcgatgtgcc cgcagaagaa
     3301 acctgcgaag cctgtgatat taccttttcc accgaggata tctcaaacct tgaggttgtt
     3361 tttcacgtga acccggaaat ccgccgggca gaaaaaaaat tctactgcgc ggcagaaccc
     3421 gcaaccaaaa ggcatatact cgtgcagagg cgcgtcgggc cgggcgcagc gctcgacctt
     3481 gaaacctcgc tcgcagcggg tgaataccgt gttcgcctcg atggcgaaaa acgctacctg
     3541 ccggtttcag ttgggggcgg taccgaacgc cgcatcgtca tagcagagaa tagctcacca
     3601 ctcaaagcgg ctgagcaacc tgtattcgtt ttcccgaacc gtggggcagt ggagcggact
     3661 ctcattattg agcggaagga tgaagataac gacgcgctca ggccggcgga agttttcggc
     3721 gtgcagctgt ttcgcgacct gttcggcaaa gagaccctgg ctcccggcct caaaattgaa
     3781 ctcggtcgcc agaatattct ttttactgat attgtcgggt cgacgaatct ctatctgagc
     3841 cgcggcgacg gcgaggcgtt cacttctgtg cgcctgcatt ttcaaaaaac ctacgaggtg
     3901 gtgcgggctt tcaacggggc ggtggtcaag actatcggtg attcagcgat ggttgctttc
     3961 accgactcgc tgcaatgcct caaagcggcc gtcgcattgc agaaaagttt caccggaaac
     4021 cctgaaacgg gtaatgtgct gctgcggatc agtattcact cgggtccatg cctcgcggtg
     4081 aacctgaaca gcggcgtgga ttactttggc aatactgtga attttgcggc aaaactgcag
     4141 cagctggctg gcgaacgcga aatcgcctat agccgcgatt ttgcacagga tccgcgcatc
     4201 agttcatacc tgcgcgagct gggtctgcag cctgagatca ggacattcag gccatcgtgg
     4261 acagatcaga cttacgatgt gccggtgctg aaaattgctt aggaggcgtc aaagcaggcc
     4321 ttttagattt gacggagcat agaactttcc ggtagctcat ggtatgcgtt tttcgttgat
     4381 tatttcctgt gcagcgaccc tggttgcggt tgcttgcaag tcgacaggcg gcacaacctc
     4441 cggtgattca ggacttcccc cgcttgttgc caaccagccg atcaaggccg acaacacggt
     4501 atccgcttat cccggcgacg ctgaagtgca ggcgatgcgc gaggcatttt ttgcgcgccc
     4561 ggtcgaagcc gaagaggtct ttcgtgtttt cgtgacttca gatggttata tgcgcaagca
     4621 gatcgcgcac accgaaacgg tgtcagtaaa ggaggacacg gggggcgacc agtccatcgc
     4681 cgacgaattc cgcaagtttg acatggttaa tagcctgcag gaaggccaga tcagggtgga
     4741 gttatacccg acgacgggta agttttaccg cgtacgccag tcaaagccgt ctatcatgaa
     4801 agagactgac aagatcatga gcgacgatat cacccgctgg caatttgttt ttgccaagaa
     4861 tgaaatacag ccgaaagatt tccgtatcag ttaccaggtc atgctgagaa aaaaaatcac
     4921 gcgcgaacag gcgctcaaga ttctcagcga acagaataaa aagaaaaagt aggaagcatg
     4981 agcgcaaacg aaaagaaaga aattattatc cgggttggcc tggatgaaaa cgcgataccc
     5041 gaatcgatca cctgggaagc tccaggtgct tcaaagggtg aagagagtgc cgatgctttc
     5101 atgctgtctc tctttagcgg cggtgaaaaa actacactgg gcattgacct gtggaccaaa
     5161 gagatgctcg tgggcgatat gaatattttt ttctaccagg cattcaaaaa gatggccgac
     5221 gtgctggagc gcgcgacaca ggataaagac gctgcgcagc tcgtgcggga tttcagccgc
     5281 gatttcggca agcaggtcaa gctgctcaaa gacgaatgac gctgtttctt gcgtcgcttg
     5341 cagtcgcggc attgcttttc ggcatgccgc tgtttatcgg tcttggagtc cttggtacac
     5401 tctaccaatt tggaaacgcc gaatcgctgg gtgccgagtc cattgctgat ctcagcaggc
     5461 agctcacggt agttttcagc gatccgctct cgaaacttac cggtgaccag agcgcgctat
     5521 ttctcacgat accctatttc acccttgccg gctacctgat agcccattcg caattcgccg
     5581 cgcgcgtggt gaacctgtac ctggtagtga tgcgcagggt cggggctttg ggtgcagtgg
     5641 gtttgggagt tatctgcgtt ctgatcgctg cgctctttac tccgctcacc ggggcatctg
     5701 gcgtgaccat agttgccatt ggtggtattc ttttcccgat tctgacaggc gccgggtaca
     5761 ccgaacgcag ggcgctgggg ttaattacgg cctcaggttc cattgggctc ttgtttagcc
     5821 ccagtctgcc tgtgatcctg tttggtatca tgagcgagaa ccgcgctgat atcaacgaac
     5881 tgtaccgcgc cggaattttg cccggtatcc tgctcatcgt tgcactcacg gttgttgtca
     5941 tcttcttcac tcttggcaga acgcagcgtg ccagcgacct gctggaacag cagttacgcg
     6001 ctgcgcagtt cagtggcagc gatgccatca agctggtctt cgagctggct gctattccgt
     6061 tgctctacgt tctgatgcac tcacggttca tgatcatcac agaactggga gttttctttc
     6121 tactgtacta tttcctgctg gaagttgttg tcttccgcga gctgggtatg aagaagctgc
     6181 tcggggtctt cgaagaagcc atcgccatgg tggggtcaat catcttgatc atctttttcg
     6241 ccatgatact taccaacgca ctgctctatg acggcgtacc gggaaaactt ttcggattca
     6301 tcagccagta tatccactcg ccgctgacgt tcctgatgct gctcaacatc tttctgctga
     6361 tcgtcggcgc actaatggat atcttttctg ccatcattgt catcgcgcct ttgatcatac
     6421 ccattgcgga gagctaccag attccgatgc tgcacctggg cattcttttt ctcacgaacc
     6481 tcgaaatcgg ctatctcacc ccgcccgtag ggctcaacct tttcctgagc tcgctgcgtt
     6541 tccagaagcc aatggctgac gtctaccgtt cgattgtgcc gtttcttatt gcgctggttg
     6601 ttgcgcagct tatcattacg tacgtgccgt ggctcagcct gtggtggaag tagccgttac
     6661 agccgcgcca ggccccgctc gagatccgct atcaggtcgc tggtgtcttc aagcccgact
     6721 gcatagcgta tgagcccgtc gggaattcct gccgcgagcc ttgcggctgc atccatgtcg
     6781 tggtagctca ttacagaagg ctggtggatc agcgattcaa ccccgccgag cgaagcggcg
     6841 atagccggaa tcttcaggct gttgagcagt gctgtggaag cggcaagtcc tgcttcctgt
     6901 gaactgtctt tcccctttac ctggaagctg acgacaccgc cgccgccgcg caggtaactc
     6961 ttcgcgagcg catgatcggg gtgggattca agcatcgggt accagacgcg tgtgactttc
     7021 gggtgtttct caagccactg cgcgatttcg agcgttgagc ggttgtgttc gcgcatgcgc
     7081 accgcgaacg atttgatacc acgcgataca agaaaacaca ggtgcgcatc ggcgttgctg
     7141 ccaaacaggc cgcggacgct ccgcaccttt tctatgagtt ctttcgaacc gagcaccgcg
     7201 ccggcaatcg cgtcgttgtg cccgctgatg aacttggtga gcgagtgcac taccaggtca
     7261 acgccgtgct ccagcgggtt aatgttcatc ggcgaggcga aagtcgcatc gacaatcgtt
     7321 gtaatgccgc ggcttctggc gaacgctgta atctctgcga gatcaaccag ccgcaggtaa
     7381 gggttggtgg ggaattctat gaagatgaca cgcgtcttct ctgtgcaggc ggttttgacg
     7441 cccgcagttg tcaagtcggc ataacttgtg ctgataccga agcgcgccag gtgctggtcg
     7501 aacatcttga acgtgccgcg ataaccctca cgcagggata ccatgtggtc gcctgcattg
     7561 gcgacgccaa aaattgccga gacaattgca ttcatgccgc tcgaggtaac gacgcagtct
     7621 tcggcatttt cgagggcaca cagggtctct tcgagcgctc gctgtgtcgg tgtaccatag
     7681 cgggcatatt cggctaagcg gtcgctgcgc ttctggtaga atgcccagag cccttcgact
     7741 tcatcgaacg cataagaagc agtctggtaa ataggtgtga caatcggctt gtgtggatcg
     7801 gcggagttgt cgtggcccgc gtgtatggcg cgtgtcgaca ggcctttttt ttcgtcggat
     7861 ttcttcatag gctagcgctg tttctccagg aagcgggcca ctgcatcttt taccgcaccg
     7921 agcgtgggct tgagatcctg cggcagattc ttgtgttcag attttgcttc aggcgcatgg
     7981 taggcgactt tgaaatcggc aaatttcagg ccatgcgccg ttgaaatcac ggcgaccgat
     8041 tcgttagcct tgatcactcc acctttcttg agtttcgtca gcgccgcgag agccacaccc
     8101 gtgtgaggat cgacaagcag gcccgcgcgg tcaccgagcg ctgccgcatc ggcgagttcc
     8161 tgttctgatg cctgctcgac aacgccgccg aattcgcgca gcgtgctgat cgcttttttc
     8221 accgaaaccg ggttgccgat acgtattgcc gaagcctgcg tttcaccggc ggtgatgggt
     8281 gagaattcag cgaagttctt cttgtacgaa aggtagagcg ggtttgccgc ttcagcctgt
     8341 gccaccgcga tgcgcggcag cgagttgacc aggccaagtt cgtaaagcat cttgaggcct
     8401 gcaccgagtg cagagacgtt gccgaggttg ccgccaggaa ttatgaacca gtcgggcagc
     8461 tgccaaccga gttgctgcgc cacttcaatg ccgacggttt tttgtccttc gatgcgcagg
     8521 ctgttcatcg agttcgcgag gtagatttct ttctcggtcg tgagttgctg cacaatttcc
     8581 atgcagccgt cgaagtcggt ttcgagtgcg agcacgagcg aaccatttgc aatcggctgc
     8641 atcagctgcg cgagtgaaat tttgtttttg ggcagcagta tgacacttaa gatacctgca
     8701 taggctgcgt aggcagagag cgcggccgag gtatcgccgg tagacgcgca ggcgatcgcc
     8761 cgaatggaag ctcccttttt gatcatgtgg tttacctgtg aaaccagcac ggtcattccg
     8821 aggtctttga atgaaccggt atggctgttg ccgcagagtt ttacgtagag ctcgtcgtgg
     8881 ttgaagcgac gcgataattc gctgcctgaa aaaagctgtg tcgcgccttc gccgagtgtc
     8941 acgacatgct cggccggaag ttcgggcaaa acccattcgc gtttgcccca gatgcccgaa
     9001 gcgtcgacgc ggttctgcga gcgccagcgg ctctcgaaca ggtcgcgcca ttcctgcgcc
     9061 gatttcttct tcagcgctgc catgtcgtgg cgcacctcaa gcaggtcgcc ggtttgcggg
     9121 cagcggtaga gtatttcgtc gagcgcgtaa cgctgctcgt tctgggggtt gatactctgg
     9181 aaccaggctt tcatctcttg cgcagacaca cgcgtgagtt tgtccccgaa acagggggac
     9241 aagaagtttc acgggcaaaa gttcttgcac ccgcgcctgc gagcgacagt aaggtggcat
     9301 gaaccacagg cagatgatac tctatgcggt actgtgtgcc gtctttatcg tgacactgcc
     9361 gtttaagccc tacgacctga gctttttgcc caaggtgctg ccgatactgc tgcttgtctg
     9421 gctggtcaat gcgcatgcca attttcgtgg ccggtggctg ctgctcttcg cactcgtttg
     9481 ctccggcgca ggcgacgtga tactcgagtt tcgctacggc ggagctttta ttgcgggcct
     9541 ggtatccttt ctggttgcgc atcttttcta catacgcctg atggtgctta cccccagccg
     9601 tgagctgaac cgctacatca ttactttgcc ggtacttgtg ccgccggcgc tggttgcctg
     9661 gctgctatgg ccccggttgg gagctttgca ggtacccgtg ctggtgtacg tgaccgtgat
     9721 cagtaccatg ctctacaccg cgctgacccg cgtctcgttt aaccgcacgc tcgtagccgg
     9781 cgctgcggcg ttcgtggttt ctgacgcggt gctggcgatc aacaagttct atgcgctgtt
     9841 tggcgcggcg ccatacacca tcatggtcac ctattatctg gcacagctgc tgcttgtccg
     9901 gggcttcctg gcggaagcgc gcggcacgcg ggcaaactga aggccccgta gagg
//
LOCUS       DAOQJF010000087         9928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3577_length_9928_cov_4.045680, whole genome shotgun sequence.
ACCESSION   DAOQJF010000087 DAOQJF010000000
VERSION     DAOQJF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3577_length_9928_cov_4.045680"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLF85_06315"
     CDS             <1..745
                     /locus_tag="PLF85_06315"
                     /inference="COORDINATES: protein motif:HMM:NF016602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19312.1"
                     /translation="FQKKPKELLFEKNKDRFAKFLPKIRHVIVDELPGFFYKWRRPDA
                     WVVSDFQKGQVVRGLHDAQGGDTVIFSDVDEIPKASAVLSATGKAGITVFEQRLYAYY
                     LNNICVDYDTHGQICVAQYNRDGLGWWRGSVMMDFDNFRKMNKSIKKMRLLHDRPEPE
                     VTVMKDSGWHFTSIGDVEKIALKLESYEHSEANTDENKNPESMRQRIREGKSIFMDNK
                     SLHKIVPLDATFPQYLVDNRDRFQHILGA"
     gene            complement(747..1625)
                     /locus_tag="PLF85_06320"
     CDS             complement(747..1625)
                     /locus_tag="PLF85_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE19313.1"
                     /translation="MSLFTKTEIERDKKDIHILIRCGIGDTLAYLARLDSLLKVYPQS
                     RVYFHVGGFRKIPEMIAELLLPDRRVYGVRLLYGYDRQSPRLEKKVLKKIREYAVPGD
                     IVESWTPDEYPLRYPLQHPFMPELFPEDHKAADEFLQKNGLRAGEALGIHPITTRGNA
                     GAFDEKRYWPQEKWHALVDYLQSKGERLLLFGAEGEEYGLKVDGQKIVSAQGLPLRTT
                     IALLTRVKGLIGTNSWCWEVTAYSGKPIISLWFTFPQNIRLYVPEKKEHTQFFTEKAV
                     TVEEVTAAYERLFTGR"
     gene            complement(1603..2469)
                     /locus_tag="PLF85_06325"
     CDS             complement(1603..2469)
                     /locus_tag="PLF85_06325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE19314.1"
                     /translation="MKKAAVVIVTYNNPTFLGICLASYKNQSHKDFAIFIADDGSRPE
                     TKAKIDEYRKILPQPITHFWHPDVGYRKAQIVNHVWRSLDSAEYPVIINVDHDTVAHR
                     RFVEDHLSQFDGSDNLLFMGRRVNLGPHVTQTYTEANVTWLNHGLPWRVIQAAWRKDV
                     EDPNRAIRISPPWLRRLLGRDRVPDLLGSNFSISNTLLRKINGYNEQYKHYWGEDGDL
                     FVRARNSGAEIRGSRSIAIQYHLYHKMLTPHPVAMENYYKVLLPGRDYKYCEQGIEGS
                     KEKLSDVIIHKN"
     gene            2628..4217
                     /gene="serA"
                     /locus_tag="PLF85_06330"
     CDS             2628..4217
                     /gene="serA"
                     /locus_tag="PLF85_06330"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNE19315.1"
                     /translation="MAKPKILISDNLDQDGVDILTASGLFEVDYRKKTSREELEAVIP
                     EVEGLIIRSASKVDKALIEKAKNLKVVIRAGVGVDNIDIPACSQKGIVVMNAPAGNSI
                     STAEQAIALMFALARKTPQAHGSMKEKKWEKTKFQGSQLTGKTLGVVGLGRIGKEVVK
                     RAKGLQMKVLGFDPYIPAENLTSLEIELVPIDTILKQSDFITVHTPLTDATKGLINEK
                     NLSLLKPGVRVINCARGGIYDEAAIVKGVKDGIIAGAGLDVFVEEPLPATSELYNFDD
                     IILTPHLGASTDEAQIEVAKESAESMVAYFKNGVARNSLNFPTIDPASMDTLAPWFDL
                     CERVGSFVGQMVKNPMKDVELSFRGEFTAVNLTPLEIALTKGLLTLAMGDEVNLVNAP
                     LLAKERSMRITARKDEKTEKEPSSMTLTVYDEKERLTLKAAVGATGGVITELNGNALK
                     LRPEGNILYILNTDTPGVVGKLGTLLGEHNINISSMELSRGKKGGEARTFLGLDDELP
                     KTLLETIQKKEFIVEARFLKI"
     gene            4276..5307
                     /locus_tag="PLF85_06335"
     CDS             4276..5307
                     /locus_tag="PLF85_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19316.1"
                     /translation="MPRPSAAGRTLRRLADFFDALAPATRFRASLGEINFVTLLITSA
                     GILTIGTVIHGSGALLWLLLFAVLFNTGLLLIFSSNNWQARKRLFFASLLALSAIPAA
                     AERLLPMFLVLLVSCYFVLLLAKQWELPHNYLNVILVLLYCYTSYGIFAATLEWRRTS
                     ALASNDGIAVDCSWQVERALVCSGESTAFDVPEFWRPAGKTHLIADLAGIIDLKTFAD
                     SATDNRIAFAAFSSPPATVMRSLTDFFNVQQKFLTSKAQGGKMLTPQGIMKSRDAELY
                     ALPYRSPEMPGYLGTYADRNALILLHQPQLEQGAGRDTTWLFVIDGTDLAAREFLLHR
                     IITGFRRSQ"
     gene            complement(5282..5767)
                     /gene="ispF"
                     /locus_tag="PLF85_06340"
     CDS             complement(5282..5767)
                     /gene="ispF"
                     /locus_tag="PLF85_06340"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNE19317.1"
                     /translation="MRIGLGYDIHRTGEGDHVWLGGVLIAAPFSLIGHSDADVLLHAI
                     TDAILGAIADYDIGHHFPPSDESNRGRPSADFLRFALERMQNEGYAITNIDCNIICEL
                     PRIAPVREEIRKKLAELMGVSVKQISVKGRTNEKVDAAGEGRAVICQAAVLLTASGEN
                     R"
     gene            complement(5846..7174)
                     /gene="trpB"
                     /locus_tag="PLF85_06345"
     CDS             complement(5846..7174)
                     /gene="trpB"
                     /locus_tag="PLF85_06345"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNE19318.1"
                     /translation="MADKKKKAPAKAKPAAAVKQAPEGYFGEFGGRFVSELLIPALDE
                     LSAAWKKYSNDKKFQKELLSLYNDYAGRPSLLTYAANLSAKWGAQVYLKREDLLHTGS
                     HKINNTLGQALLASKMKKKRIIAETGAGQHGVATATAAALMKIPAHVYMGSEDLRRQA
                     LNGFRMRLLGATVSGVGGDHGTLRDAVNEALRDWAKNVDDTYYLIGSAIGPHPYPTMV
                     RTFQSVIGREARAQIMKATGKLPQAVFACVGGGSNALGMFYGFLKDTKVQLIGAEAGG
                     RSLTPGNHSASLTAGKPGVLQGALTYIIQSADGQVQDVHSISAGLDYPGVGPEHAYLL
                     QQKRTEYVAVRDKAALDAFQELAQEEGVIPALESAHAVAAAKNYVNEMRKAGKKNPLI
                     LICLSGRGDKDVQEVERLLALPQESEQPEESAPATQFNTAPVVKVVAAGE"
     gene            complement(7187..7453)
                     /locus_tag="PLF85_06350"
     CDS             complement(7187..7453)
                     /locus_tag="PLF85_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE19319.1"
                     /translation="MPAFEIDEQLSALTAAGQYKAIADLTGVAHISSSVLGVLARFAE
                     ECRRNHGELRLVVTEAGVANLLQVTMLDKVFEIYNSLEEAEEDF"
     gene            7707..9497
                     /locus_tag="PLF85_06355"
     CDS             7707..9497
                     /locus_tag="PLF85_06355"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE19320.1"
                     /translation="MSSRSFRRRAAFTVLSGLALFLLQCSSTEKRKLPTPDATEGSQF
                     TFTKSDRLNCSMAGADSLCLETNQLAATAASLMPGSSVTPNTVFIDRSFVTNGDYSRC
                     VADGQCVTNSYVKGKKAAVSAATAPALGLPFESAMMYCAWAGKRMPTEAELSLAATLP
                     GFAAAPQREWTNDWTVNDESQCTVTDGKVKKNICVPQNPMGICGGAFGCSKWLRDKKI
                     FDAAGRTSAVQSINSKNETAFRCVAENAPPTAAPPWMIAKAPAPLADPAPPTEAQLRL
                     LHNIGETDVLNKPICKAKYTSPAHCKDPVTYFTPNESQNYMFAPYIRNLGGGYAGVAA
                     DANYSYIALAKSRFVWLFDFDVNINALHRIIRAMVLESETVTQFLDRFSKKQQKASLE
                     ILAKYYSDRNDLDFIQQVFRNNYDSLGKHYRRSATPDKKQGDFGWLRHAEHYRYIRLL
                     YQQNRIAIVPGDMLKEKSIRAIGRSARALGVPIRVYYPSNAEEFWDYSTNDSNYKKNV
                     LSLPFDEASITFRSVHEYPWHPAHRKGFKGFWHYVVHGAYNYQKRLLVPHYDYMDYFK
                     MHRVVSKDRSDFSTIEIPAAIPAGILEQGY"
     gene            9641..>9928
                     /locus_tag="PLF85_06360"
     CDS             9641..>9928
                     /locus_tag="PLF85_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE19321.1"
                     /translation="MRCIPAGTFIRGSDNHLADEKPAQKIHISSFYMDTYEVTNADFQ
                     QCLDAGKCRECLKNKTCDYIGARYGWRYKKPKQPVSGVSWFTAREYCEFRGK"
BASE COUNT         2274 a         2832 c         2651 g         2171 t
ORIGIN      
        1 cttccagaaa aagccaaagg agctcttgtt tgaaaagaac aaagatcgct tcgcaaagtt
       61 cttacctaag atcaggcatg tgattgtcga cgaactgccc ggtttcttct ataagtggcg
      121 ccgccccgac gcgtgggttg tgagcgactt ccaaaaaggc caggtggtac gcggtcttca
      181 tgatgcccag ggcggcgaca ccgtgatctt ctctgacgtc gatgaaatac cgaaagcaag
      241 cgcagtgctc tctgcgacag gtaaagccgg catcactgtc tttgaacagc gtctctatgc
      301 ctactacctg aataatatct gcgtagatta tgacacccac ggacaaatct gcgttgcgca
      361 atacaaccgg gatggcctcg gctggtggcg gggatcagtc atgatggatt tcgataactt
      421 ccgcaagatg aataagtcga taaagaagat gcgactgctg catgacaggc ctgaaccgga
      481 agtcaccgtt atgaaagact cgggctggca ctttacctcg atcggagatg tcgagaagat
      541 tgcgctcaaa ctcgaatcgt acgagcactc agaagcaaat accgacgaga acaagaaccc
      601 cgaatcgatg cgccagcgca tccgcgaagg caagagcatc ttcatggata acaagagcct
      661 gcataagatt gtaccgctcg atgcaacttt tccgcaatac ctggtcgaca accgcgaccg
      721 gtttcagcat attttaggag cttaatctaa cgacctgtaa acaagcgctc gtacgcagca
      781 gtaacctctt caacagtcac cgctttttcg gtaaagaact gcgtgtgttc ctttttctca
      841 ggtacataga ggcgaatatt ctgcggaaaa gtaaaccaca gtgaaatgat cggcttaccc
      901 gaatacgcgg ttacctccca gcaccacgaa ttggtgccga tgagtccctt cacgcgggtc
      961 agcagcgcga tcgtagtgcg caatggcagc ccctgcgcag acacaatttt ctgtccgtct
     1021 accttgagac cgtactcttc gccttcggca ccgaacagca gcagccgctc ccccttgctc
     1081 tgcaggtaat cgaccaatgc atgccatttc tcctgcggcc agtagcgctt ctcgtcgaat
     1141 gccccggcat tgccgcgggt agtgatcggg tgtatgccca gcgcctcgcc ggcccgaagt
     1201 ccatttttct gcaaaaactc atcggcggcc ttgtggtctt cgggaaaaag ttcaggcata
     1261 aaaggatgct gcagcgggta acggagagga tattcgtcag gcgtccagct ttcgacaatg
     1321 tcgcctggca ccgcgtactc gcgtattttt ttcagaactt ttttctcgag gcgcggcgac
     1381 tgcctgtcgt agccgtagag caggcgcaca ccgtaaacgc ggcgatccgg caacaatagc
     1441 tccgcaatca tttcgggtat cttgcgaaat ccccccacgt gaaaatagac cctcgactgc
     1501 gggtaaactt tcagcaagct atcaaggcgc gccaggtagg ccagcgtatc gccgatgcca
     1561 cagcggatga ggatgtgaat atctttctta tcgcgttcga tttcagtttt tgtgaataat
     1621 gacatcgctc agtttctcct tgcttccctc aataccctgt tcgcaatatt tgtaatcgcg
     1681 acccggtaac agcactttat aataattctc catcgccacc gggtgaggcg tcagcatctt
     1741 atggtacagg tggtactgta ttgcgatgga gcgcgagccg cgaatttctg cgccactgtt
     1801 gcgcgcgcgc acgaacagat cgccgtcttc gccccagtag tgcttgtact gctcattgta
     1861 gccgttgatt tttctcagca gcgtattgct gatcgagaaa tttgaaccga gcagatcagg
     1921 cacccggtcg cggcccagca atcgacgtag ccacggcggc gatatgcgaa tcgcccggtt
     1981 ggggtcttca acatctttgc gccacgctgc ttggatcacc cgccatggca gcccatggtt
     2041 gagccaggtg acattcgctt ctgtataggt ctgcgtgaca tggggtccca ggttgacccg
     2101 gcgtcccatg aagagcaggt tatcagaacc atcaaattga cttaagtgat cttcgacaaa
     2161 acggcggtgc gccacggtat cgtgatcgac gttgatgata accgggtatt ctgccgagtc
     2221 gagcgacctc cagacatggt taacgatctg cgccttacga tagccgacat cggggtgcca
     2281 gaagtgcgta atcggttgcg gcagtatttt gcggtattcg tcgattttgg ctttggtctc
     2341 gggacgcgaa ccatcgtcgg caataaaaat cgcaaaatct ttgtgggatt gattcttgta
     2401 agaagccagg cagatgccga gaaacgtggg attgttatac gtaacgatga ctacggctgc
     2461 ttttttcatg gcactcgatc ttcattagcc cctagaccaa aggcgcacaa atattcacaa
     2521 cgtggaaaga agaaatcgcc attttctggc tgtcccgtca tggatgtgtg cgcagcgacc
     2581 ctttttattt acgccgtgtc gcaaagcaca attagggtgg atttcatatg gcaaaaccta
     2641 agattctgat ctctgacaat ctcgaccagg acggcgtcga tatcctcacc gcctcgggcc
     2701 tctttgaagt cgattaccgc aagaagacca gccgcgaaga gctggaagcg gttattcccg
     2761 aagtggaagg tctcattatc cgctcggcca gcaaggttga taaggcgctc atcgagaaag
     2821 cgaagaatct gaaggtggtc atccgcgccg gtgtgggcgt cgacaacatc gacattccgg
     2881 cctgctcgca aaagggcatc gtggtgatga atgcacccgc cggtaactcc atttcaacgg
     2941 ctgaacaggc aattgctctc atgtttgcgc tggcccgcaa gaccccgcag gcgcatggtt
     3001 ccatgaaaga gaaaaaatgg gaaaagacaa agttccaggg tagccagctg accggaaaaa
     3061 ccctcggcgt ggttggcctt ggacgtattg gtaaagaagt ggtgaagcgc gccaagggcc
     3121 tgcagatgaa agtactgggt ttcgacccgt acatacctgc agagaacctg acttcactcg
     3181 aaatagaact cgtgccgatc gacacaattc tcaagcagtc tgacttcatc actgtccaca
     3241 ccccgctgac agatgcaaca aaggggctca tcaatgagaa gaacctttcg ctgctcaagc
     3301 caggcgtgcg cgttatcaac tgcgcgcgcg ggggcattta tgatgaagca gcgatcgtga
     3361 aaggcgtcaa ggacggcatc attgccggcg caggtctcga cgtgttcgtc gaagaaccgc
     3421 tgccggcaac ttcagaactc tacaacttcg atgatatcat tctaactcct catctcggcg
     3481 cctcaacaga tgaagcgcag atcgaagtgg caaaagagag cgccgagtct atggttgcct
     3541 acttcaaaaa tggtgtggcc cgtaactcgc tgaacttccc gactattgat cctgcatcga
     3601 tggatacact ggcaccctgg tttgacctgt gcgaacgtgt cgggtctttc gtcggccaga
     3661 tggtgaagaa cccgatgaaa gatgttgaac tgagcttccg cggggaattt accgcagtca
     3721 acctgacccc actcgaaatc gcactgacca agggattact caccttagcc atgggcgacg
     3781 aggtcaacct ggtcaacgcg ccattgctcg caaaagagcg ttcgatgcgc atcaccgcgc
     3841 gcaaagacga aaagacagaa aaagaacctt cgagcatgac gctgaccgtc tatgacgaaa
     3901 aagaacgcct gactctcaag gcggcagtcg gtgccaccgg cggagtgatc acagaactca
     3961 acggcaatgc gctcaaactg cgcccggaag gtaacattct ctatatcctg aacaccgaca
     4021 ccccgggcgt cgtgggcaaa ctcggtacgc ttttgggcga acacaacatc aatatctcga
     4081 gtatggaact ttcgcgcggc aagaagggtg gcgaagcacg gacctttttg ggtctcgacg
     4141 acgaattgcc caagacgctg ctcgaaacga tacagaaaaa agaatttatt gttgaagctc
     4201 ggttcctgaa aatctgaagc accgggtgtg gccgacaaag aggcgggtag caattcaata
     4261 acgaacgggc aaactgtgcc ccgcccgtct gctgccggcc gcacgctgcg gcggctggca
     4321 gacttctttg acgccctggc tcctgcaacc cgcttcaggg cctcgctcgg cgaaatcaat
     4381 tttgtcacgc tgctgatcac gtcggctggt atactcacga tcggcaccgt gatacacggc
     4441 agcggcgcac tgctttggct actgctcttc gcggtgctct ttaataccgg gttactactc
     4501 atctttagca gcaataactg gcaggccagg aagcgcctgt ttttcgcctc gctgctcgcc
     4561 ctttcggcaa tacccgccgc cgccgagcgg ctattgccca tgttccttgt tctgctcgtc
     4621 agttgctact ttgtgctcct gctcgcgaaa cagtgggagc taccccacaa ctacctgaat
     4681 gtaattcttg tcttgctcta ctgctacacg agctatggca ttttcgccgc gacacttgaa
     4741 tggcgccgca ccagtgcgct cgcatcaaac gatggcatcg cggtagattg cagctggcag
     4801 gtggaacgtg ccctcgtctg tagcggcgaa tcaactgctt tcgatgtgcc cgaattctgg
     4861 cggcccgccg gtaaaacgca cctgattgct gacctggcgg gaattattga cctcaaaaca
     4921 tttgctgatt cggcgactga taaccgtatc gcctttgccg ctttctcttc gccgccggcc
     4981 accgtcatgc gctccctgac ggattttttc aacgtgcagc aaaaatttct caccagcaag
     5041 gcgcagggcg gcaagatgct gacaccgcag ggtatcatga aaagccgcga cgcagaactc
     5101 tacgcgctac cctaccgctc acccgaaatg cccggctatc tcggcaccta tgccgaccgc
     5161 aatgctttaa tactgttgca ccagccacag ctggagcaag gcgccgggcg agacaccacc
     5221 tggctttttg tgattgatgg caccgacctt gcggcgcgcg agttcttgct gcaccggata
     5281 attaccggtt ttcgccgctc gcagtaagca gcacagctgc ctggcagatt acagccctgc
     5341 cttcgccggc agcatcaacc ttttcgtttg tgcggccctt gacgctaatc tgttttactg
     5401 atacgcccat taattctgcc agctttttgc gaatttcttc gcgcacaggg gcaatgcggg
     5461 gcagctcgca gataatattg caatcgatgt ttgttatcgc ataaccctcg ttctgcatac
     5521 gttcgagcgc aaaacgcagg aagtctgcgc tcgggcggcc ccggttactc tcgtccgatg
     5581 gcggaaaatg atggccaata tcgtaatcgg cgatcgctcc gagaatggca tcggtgatgg
     5641 cgtgcaaaag tacgtcggca tccgaatggc cgatgagtga aaagggcgct gcgataagca
     5701 caccaccgag ccagacgtgg tcgccctcgc ccgtgcggtg gatgtcgtag cctaagccta
     5761 ttctcatatg accggcggtc acttcggctg cgctcagtgg ccgctgcaga cgtgcggtga
     5821 gcgcagtcga accgtacgca gtgagttact cacccgccgc gacaactttc acgacgggtg
     5881 cagtgttgaa ctgtgtcgca ggtgcactct cttcgggctg ttcgctctct tgcggcagcg
     5941 caagcaggcg ttcgacttcc tgcacgtctt tgtcgccccg gccagacagg caaatcagga
     6001 tcagcggatt cttcttaccc gccttacgca tttcattgac gtagttcttt gccgccgcga
     6061 ccgcgtgcgc tgattcgagc gcgggaatca cgccctcttc ctgcgcgagc tcctggaaag
     6121 catcgagcgc tgccttgtcg cgcacggcga cgtactcggt gcgtttctgc tgcagcaggt
     6181 acgcatgctc gggaccaacg cccgggtaat cgagtcccgc agatatcgaa tggacgtcct
     6241 gcacctggcc gtcggcgctc tggatgatat aggttaacgc gccctgcagc acaccgggtt
     6301 taccggcagt gagcgaagcc gagtggttgc ccggcgtgag gctgcgacca cctgcttcgg
     6361 cgccgatcag ctgcaccttg gtgtccttga gaaaaccgta gaacataccg agcgcgtttg
     6421 agccaccgcc gacgcaggcg aagacggcct gcggcagttt gcctgtcgct ttcataatct
     6481 gcgcgcgcgc ctcgcggccg atgaccgact ggaaggtgcg caccatcgtc gggtacgggt
     6541 gcgggccgat ggccgaaccg atgaggtaat aagtgtcatc gacattcttt gcccagtcgc
     6601 gcaatgcctc gttaacggca tcgcgcagtg taccatgatc accgcccacg cccgacacgg
     6661 ttgccccgag caggcgcata cgaaaaccgt tcagtgcctg gcggcgcagg tcttcggagc
     6721 ccatgtagac gtgtgctgga attttcatca gcgccgcagc agttgccgtg gcaaccccgt
     6781 gctggccggc gcctgtctca gctatgatgc gcttcttttt catcttggac gcgagcaacg
     6841 cctggcccag ggtgttgttg atcttatgcg aaccggtgtg cagcaggtct tcgcgcttga
     6901 gataaacctg agcgccccat ttcgccgaaa ggttggccgc gtaagtgagc agcgaaggcc
     6961 ggcctgcata gtcgttataa agactgagca gttctttctg aaatttttta tcgttgctgt
     7021 attttttcca ggcagcgctg agttcatcga gcgcgggtat caacagctca gagacgaatc
     7081 ggcctccgaa ttcgccgaaa tagccctcgg gagcctgttt cactgccgca gcgggtttag
     7141 ctttcgcggg cgctttcttt tttttgtcag ccatagttgc ctcagtttaa aaatcttcct
     7201 ctgcttcttc gagggagtta taaatttcaa agaccttgtc gagcatggtg acctgcaaca
     7261 ggttggcaac gccggcttcg gtcactacca gccgcagctc gccgtggtta cggcggcatt
     7321 cttcggcaaa ccttgcgagc accccgagca ccgaactcga aatgtgggcg acgccagtca
     7381 ggtcagcaat ggccttgtac tgcccggcag ccgtgagtgc cgagagctgc tcgtcaatct
     7441 caaaagccgg catgctttta gtgagacctg tcagtgataa aactgcaaaa gatttgcgcg
     7501 cagccgactt cacaggacaa ccccggcaag agagaaaagc gatcgggtgg cctctccgtt
     7561 tttcatggac atcagtggcg gtcagccggc gacattatgt cacatcttta atacaggtca
     7621 agcacataat gagcgccaac gaagccggca aatatgctca ctgtacaaga tttgaccccc
     7681 tgaaccgggc tttacccctg acaaaaatgt cctcacgatc ttttcggcgc cgcgcggcgt
     7741 tcacagtgct ttctggcctg gcgctttttc tgctccagtg ttcatccaca gaaaagagga
     7801 agctccccac ccctgatgct accgagggta gccagttcac gttcaccaag agcgaccggc
     7861 tgaactgctc catggcgggc gccgatagcc tgtgcctcga aaccaaccag cttgcggcca
     7921 ctgcagccag cctcatgcca gggagcagcg tgacgcccaa cacagttttt atcgaccgtt
     7981 catttgtcac caacggtgac tattcgcgct gcgtggctga tggccagtgc gtcacgaatt
     8041 cctacgtgaa aggcaagaaa gcagcggtga gtgcggcgac tgcccccgca cttggcctgc
     8101 cgtttgaatc ggcgatgatg tactgcgcgt gggcgggtaa acgtatgccc actgaggcgg
     8161 agctctctct tgctgcgacc ctgcccggtt ttgctgctgc cccccagcgc gagtggacca
     8221 atgactggac cgtcaacgat gaatcgcagt gcaccgtcac cgacggaaaa gtaaagaaga
     8281 acatttgcgt gccacagaac cccatgggca tatgcggcgg tgcatttggt tgcagcaagt
     8341 ggctgcgcga caaaaagata tttgatgccg ctggcagaac cagcgcagtg caatctatca
     8401 acagcaaaaa tgaaacggca tttcgctgtg tggcagaaaa cgcaccgcct accgcggctc
     8461 cgccgtggat gattgcgaag gcccctgctc cgcttgctga cccggccccg ccaacagaag
     8521 cacagctcag gctgctgcac aacatcggcg aaacggacgt actcaataaa ccgatctgca
     8581 aggcaaagta cacatcccct gcgcactgca aagacccggt gacatacttc acgcccaacg
     8641 aatcacagaa ctacatgttc gcgccgtata tccgcaatct cggcggcggg tacgcaggtg
     8701 tcgcggcaga tgcaaactat tcgtacatcg cgctcgccaa aagccgtttc gtctggctgt
     8761 tcgacttcga cgtgaacatc aatgcactgc accgcattat ccgcgcgatg gtactggaaa
     8821 gcgaaacagt tacgcagttt ctcgaccgct tcagcaagaa acagcagaaa gcctcgctgg
     8881 agatactggc aaagtattac agcgatcgca atgatctcga cttcatacag caggttttcc
     8941 gcaacaatta cgactcgctc ggcaaacact accggcgcag cgcaacacct gacaagaaac
     9001 agggggactt tggctggctg cgccatgccg agcattatcg atacatacgc ctgctctacc
     9061 agcagaaccg tattgccatt gtaccgggag acatgctgaa agaaaagtcg attcgcgcca
     9121 tcggtcgttc ggcgcgtgca ctcggcgtac caatacgcgt ctattacccg agcaatgccg
     9181 aagaattctg ggattacagc accaacgact cgaactacaa aaagaacgtg ctctcactgc
     9241 cgttcgacga ggcttcgatc actttccgct cggtgcatga atacccctgg cacccggcgc
     9301 accgcaaggg tttcaagggc ttctggcact atgtcgtgca cggcgcgtac aattaccaga
     9361 aaagactgct cgtaccccac tacgattaca tggactactt taagatgcac cgcgtagtca
     9421 gcaaagaccg gtccgacttc agcacgattg aaattcctgc agctataccc gcaggaatac
     9481 tagagcaagg ttactgaaaa tgcaaatgcg cagcattacc tccagcggcc ggaaccggcg
     9541 ggtattcatc gtcccggtga ttgcactttt ggcttcggtt ttcgcggcat gttcgggcag
     9601 ctcggcagcg agcaccggct gcggtcccga ggttgcaggg atgcgctgta ttcccgcagg
     9661 aacttttatt cgcggttctg ataaccacct cgcagacgaa aagcctgcgc aaaaaatcca
     9721 tatctcttct ttttacatgg atacctacga ggtgaccaat gccgatttcc agcagtgcct
     9781 cgacgccggc aagtgccgcg agtgcctgaa aaataagaca tgcgactaca taggcgcgcg
     9841 ctatggctgg cgctacaaga aaccgaaaca acctgtttca ggcgtcagct ggttcaccgc
     9901 gcgcgagtac tgcgagttcc gcggcaaa
//
LOCUS       DAOQJF010000088         9896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3600_length_9896_cov_5.734783, whole genome shotgun sequence.
ACCESSION   DAOQJF010000088 DAOQJF010000000
VERSION     DAOQJF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_9896_cov_5.734783"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLF85_06365"
     CDS             complement(<1..633)
                     /locus_tag="PLF85_06365"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE19322.1"
                     /translation="MQDVAIPPLPTIVAQVIRFDANDPKNGAAELEKLILPDRAISAD
                     LLRVSNSAFYGRSGKVKSLKDALAVLGVKATKNLVVYLSTRAMASAYKSDTFRKYLNR
                     YPIFAALTAQAVASGTTMKAQAEEGFLAALLHSIGMNLLALQREGHYSDMLAACEKNK
                     WNLAQLEKQSYGVTHTELGKKAAVHWSLPEIYQKLMEITPSTDAGTLTEPM"
     gene            complement(746..1189)
                     /locus_tag="PLF85_06370"
     CDS             complement(746..1189)
                     /locus_tag="PLF85_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNE19323.1"
                     /translation="MYPILISVHLLAATIWTGGHIVLFAAVLLPALRTRDHRRITDFE
                     QHFEKIGIPALVTLVVTGLWMASLQLPEVSQWFTLQSPSSRTVSAKLFLLLITLTLAL
                     HARLRLIPRLSEKTLPNLAVHITLVTLTSILFALTGVMHRFGGLW"
     gene            complement(1189..1599)
                     /locus_tag="PLF85_06375"
     CDS             complement(1189..1599)
                     /locus_tag="PLF85_06375"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNE19324.1"
                     /translation="MPEWRSEYTVGVPEIDRQHQYLFGLGARLGRTAAANGTKDDLAE
                     IVKDIVAYANDHFTFEEKVMEQAGYENLAYHRAMHEFMRTRLGLLQAQLAKGFLSRDE
                     LTEFMETWLTEHIIMEDMRYIPSVAAQYAQGGYP"
     gene            complement(1592..2008)
                     /locus_tag="PLF85_06380"
     CDS             complement(1592..2008)
                     /locus_tag="PLF85_06380"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNE19325.1"
                     /translation="MEMLEWNANYATGIPEIDSQHSYLFELANRFIRSLSGLPDPVAQ
                     RQLLAELTLYTQRHFTFEEKCMEEAGYAHLAEHRESHQRLRVQLTRFASDVESGRLDP
                     RELAEFLRNWLKLHVLREDMRYIPALTTQGEQNGNA"
     gene            complement(2018..3229)
                     /locus_tag="PLF85_06385"
     CDS             complement(2018..3229)
                     /locus_tag="PLF85_06385"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNE19326.1"
                     /translation="MILIKGDGLDLPHLAGMFHWPVFATAFRVFFLATGLHALFIISV
                     WLAKLTGSDILPTAIAGTWWHSYEMVFGFCRAAILGFLFTAGQNWCGQQMLTRGPLAI
                     VFTSWLLGRVAWFLPAPLVYFGAGIDILGGAFALTRMKPLLQPVQKHNHKVVYLHGIF
                     IAVQLLALIALLTPQALIPVQHSIHMGLLVVLAFIVIIAGRILPFFTSLAIPEAKPRI
                     SQRLENSIFPMTLFLIAVYAALPLHDSLRYAAAAAILAFAGLNTVRWLNWNPLASWRK
                     PILAILFSGYLWLLLGLMMLAASLVSGAAASPAWHVLGIGSAAIFIFGMMTRVSLGHT
                     GRKIHASPLINAGYVLIHGALVLRVALVLAGLPLAAYTWSAALWLGAFSLFTFIYATI
                     LWQPRIDGKPG"
     gene            complement(3230..3820)
                     /locus_tag="PLF85_06390"
     CDS             complement(3230..3820)
                     /locus_tag="PLF85_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE19327.1"
                     /translation="MKRMIILISMFALANAVIRCKKEVDPHAHHKMEAGKVSGASLYQ
                     FHDMFTDQNGRQLMLHQLQGKYVVVAMFYATCTAICPRIAAEMLRLEKNLSSAQRANT
                     TFLMISFDPDRDSPANLQKFIAKMKLTDSFVLLTGKAESIREVAAALGVNYKKQDDGE
                     FAHSSLFSLLSPQGEIILQHAGLSVSVDNFREKIRP"
     gene            complement(3820..4494)
                     /locus_tag="PLF85_06395"
     CDS             complement(3820..4494)
                     /locus_tag="PLF85_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNE19328.1"
                     /translation="MVRIPAGSFRPFDLRKEQKPVKVPSFVLDKYPVSVAQYAAFVAA
                     HPAWQRGNAPPIKADQGYLKTINLSQPSNSVVMTQVSWYAARAYCAAQGKRLPTLYEW
                     EYVATFNKWDQRSDANSRILAWFGKPNDQTTGGTIGSGLVSPQGVHDLHGLIWEWVDD
                     FNSSTVTGDSRADTDTESNLFCGATSLSGSDLRNYATYMRYGMRSSLKGNYTGNNLGF
                     RCARDK"
     gene            complement(4560..5975)
                     /gene="nirK"
                     /locus_tag="PLF85_06400"
     CDS             complement(4560..5975)
                     /gene="nirK"
                     /locus_tag="PLF85_06400"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HNE19329.1"
                     /translation="MKSKKTLSHGAATLFLLALAVPTFIACGTKTEKAVLTHAPEVPK
                     PIDRSGNAKVIVELETKELKGRLADGVEYTFWTFGGTVPGPMIRVREGDDVEFTLSNH
                     PTSKMPHNIDLHAVTGPGGGAAASLTSPGHSSKFSFKAINPGLYVYHCATAPVGMHIA
                     NGMYGLILVEPKGGMPKVDKEFYVMQSEFYTKGKNGQAGLQPFDMNKALDERPEYVVF
                     NGSVGSMTGDKALKTKVGETVRLYVGNGGPNLVSSFHVIGEIFDHVYTEGGTEKNQKN
                     VQTTLVPAGGSAIVDFKMDVPGNYVIVDHSIFRTFNKGALGILKAEGPENTAIYSGKQ
                     ADTVYLGEGGATQTMPAEKDMKVVAKSKEERIAEGKRIYGTTCQACHMNEGQGVPKAF
                     PPLAKSDYLNADKVRSIKVVLEGKSGPIVVNKEKYDSVMPALGLSDEDVANVLSFVLN
                     SWGNSGGVVTPQEVAAQRSVH"
     gene            6164..6775
                     /locus_tag="PLF85_06405"
     CDS             6164..6775
                     /locus_tag="PLF85_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE19330.1"
                     /translation="MNTIDFGDLAPTSADKLRAIAHYRRFSRNEAVFREGDRYAGPFL
                     VTEGQFKIYMLGDEGKEAIMHIFRPGELIAGGPLFLGGNYPAYCAALSDGCLVAFEYD
                     RLKKLLVTDDAVNGFFVGKSVRLIPRLKDKIENLTLKNAEGRIYAYLKSLGADKAPIE
                     LDIPKNQIAALLDLTPESVSRICNQLISKGILVVEGKTYRLQP"
     gene            complement(6802..7344)
                     /locus_tag="PLF85_06410"
     CDS             complement(6802..7344)
                     /locus_tag="PLF85_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNE19331.1"
                     /translation="MAIKSTIFKADLQVTDTDRDYYQSHKLTLARHPSETDERMMLRL
                     LLFAIHADPALEFAGGISTEDTPALWRKNLSGEILEWIEIGQPDEKRIRKACGRARKV
                     YVYCYGAHRAKPWWQSIKASLDRFDNLCVKFLPDDQVRPLSGMSERTMQLQCMIQDGD
                     ISFSDHGRETTITPEVWKES"
     gene            complement(7401..7772)
                     /locus_tag="PLF85_06415"
     CDS             complement(7401..7772)
                     /locus_tag="PLF85_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNE19332.1"
                     /translation="MQPELPLVYSCSGCSSAAQMANSIALRLDREGHAEMSCIAGVGG
                     GVKPLVRTAKSGRPIIALDGCALKCVEHCLAHHGVVAKRHFVLSEYGVEKKKHHLFSE
                     DEISQAYGQVLRELDRSPAIF"
     gene            complement(7762..8772)
                     /locus_tag="PLF85_06420"
     CDS             complement(7762..8772)
                     /locus_tag="PLF85_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNE19333.1"
                     /translation="MQATRPESHRAITNWLFVCAAVVFAIVVIGGITRLTRSGLSIVE
                     WKPVSGVVPPMGETEWLAEFEKYKATPEYRLVNHSMTLPEFKNIFWWEFIHRIFARLV
                     GVLFFLPFIWFLVRRRIDRTLALRLGVIFALGGLQGFLGWYMVKSGLVNDPHVSHLRL
                     TSHLMTAVLIYALMMMTAWSLEKRPGLPGSRRLAWANVILVFVMMASGALVAGLKAGK
                     MYNTFPLMNGQYFPQGALGFSPVLSNFSDNPIMIQFIHRNFAYALGIFFFWQLWRERK
                     NISLGHPLALIVMAYMLQFVLGVFTLLKAVPVSLGALHQANALVLFTATLFLLARRPH
                     AT"
     gene            complement(8753..9523)
                     /locus_tag="PLF85_06425"
     CDS             complement(8753..9523)
                     /locus_tag="PLF85_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19334.1"
                     /translation="MRISTRIWILATTVLTLASLVIKLYFLPAAERFTRLTVEEQIHR
                     EMQAFEKIIGVVVTAANGDKEYVRRALYAISADKTIPIELRRSEFLDSQFGKRQDRQP
                     QNDFEKRVMYTAEAAFRQTEQFIEYAYPLKAQPVCQTCHVGADGKPITLGQPVGIAIR
                     RLPLTAITDSRISYFTLDLFWENFGLLVVCVLLVLLPVWLWILRPLHQLAEQSEEILH
                     RRDMADDSDKAILLTEGEKMPEEWRSIRRLIDDASDKT"
     gene            complement(9520..>9896)
                     /locus_tag="PLF85_06430"
     CDS             complement(9520..>9896)
                     /locus_tag="PLF85_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19335.1"
                     /translation="RGKKKLMIRRNKGTFISILIGLWGLSAANGIKDFYFEMRAKTRG
                     EIISYSSLQEFCGWTGEKQPGETEFDYGMRRARNMFSALMAMSFMSVLFFGSVIGVIF
                     GAFWLAGGFKDDDENEGSNTLT"
BASE COUNT         2212 a         2607 c         2785 g         2292 t
ORIGIN      
        1 cattggttcg gtaagcgtgc cggcatccgt cgatggtgtg atctccatca gtttctggta
       61 aatctcaggc aggctccagt gtaccgcagc tttcttgccc agctcggtat gggtgacgcc
      121 ataagactgc ttttccagct gcgccaggtt ccatttattt ttttcacagg ccgcgagcat
      181 atcgctgtag tggccctcgc gctgcagtgc aagcaggttc atgccaatac tgtggagtaa
      241 agctgccagg aaaccttctt ctgcctgcgc cttcattgtt gtcccgctgg ccacggcctg
      301 ggccgtcagc gccgcaaaga tggggtaacg gttgagatac ttgcggaagg tgtcgctctt
      361 gtatgccgaa gccatcgcgc gcgtacttag gtagaccacg aggttcttgg ttgcttttac
      421 ccccagcacc gcgagcgcat ctttgagtga tttcaccttg cccgagcgac cgtaaaacgc
      481 cgaattcgat acgcgcagca gatcggcgct aattgcccgg tcagggagta tcagcttttc
      541 cagttcggct gcaccattct ttgggtcatt tgcatcgaat cgtattactt gcgcgacaat
      601 ggtcggtaat ggtgggatag caacatcctg cattaacgca cgcacccgga cgcattcctg
      661 ttgcgaaaag cctttttctg aagcggaaaa tccctctaaa acaggtgaag gttagtcaca
      721 ggctaacagg ccgttgtcag ggcttttacc aaagaccgcc aaaccggtgc atgacacctg
      781 taagtgcaaa aagtatacta gtgagtgtaa ccagtgtaat atgcaccgcc aggtttggca
      841 gagtcttttc gctcaatcgg ggaatcaacc tcagccgtgc atgcagtgcc agtgtgagag
      901 taatcagcag aaggaataat ttcgcagaaa cggtgcgtga tgaaggtgat tgcagggtaa
      961 accattgcga aacttcgggt agttgaagcg atgccatcca taagccggtt accacgagtg
     1021 tcacgagcgc tggtatgccg attttctcaa aatgctgctc gaaatccgtg atgcggcgat
     1081 ggtcgcgcgt tctgagcgcg ggtagcagca ctgctgcaaa aagtacgatg tggccgccgg
     1141 tccagattgt cgccgcgagc aggtgcacac tgatcagtat ggggtacatt aggggtagcc
     1201 tccctgcgca tattgggcag cgacagaagg tatgtagcgc atgtcttcca tgatgatatg
     1261 ttcggtcagc caggtttcca tgaattcagt gagttcatcg cggctcagga aacctttagc
     1321 gagctgtgcc tgtaacaggc ccaggcgcgt tctcatgaat tcgtgcattg cccggtggta
     1381 ggcaaggttt tcgtaaccag cctgctccat gactttttcc tcaaaggtga aatgatcatt
     1441 ggcataggct acgatgtctt tgacaatttc ggcgaggtcg tctttggtgc cgtttgctgc
     1501 cgcagtgcga ccaagtcttg cgcccaggcc gaacaggtac tggtgctgcc ggtcgatttc
     1561 cggtacgcct acagtatatt cagagcgcca ctcaggcatt tccattttgc tctccttgtg
     1621 ttgttaaagc cggaatataa cgcatgtctt cgcgcagcac gtgcagtttg agccagttac
     1681 gcaagaattc tgcaagctcg cgcgggtcga gccttccgga ctccacatca gaggcaaacc
     1741 gggtaagttg cacccgcagg cgctggtgcg actcacggtg ctccgcaaga tgcgcatagc
     1801 ctgcttcttc catacatttt tcttcaaagg taaaatggcg ctgtgtgtac aacgtgagct
     1861 ctgcaagcag ttgccgttgt gcgaccgggt ctggtagtcc actcaggctt cgaataaaac
     1921 ggttagccag ctcaaagaga tagctgtgct gtgagtcaat ttcgggaatt cccgtcgcgt
     1981 agttggcgtt ccattcgagc atttccattt tgagatccta acctggttta ccgtctattc
     2041 ggggttgcca gagaatagtt gcataaataa aagtaaaaag gctgaatgca cccaaccaga
     2101 gcgcagccga ccacgtgtag gctgcaagtg gcaatcctgc caggacgagt gccacgcgca
     2161 agacgagagc gccgtgaatc agtacgtaac ctgcgttgat gagcggactt gcgtgaattt
     2221 tcctgccggt atgcccgagt gaaacccgcg tcatcatacc aaaaataaaa atcgcggcag
     2281 agcctatacc gagcacatgc cacgccggtg aagccgctgc accgctcacc agcgacgcgg
     2341 ccagcatcat gaggccgagt agcagccaga gatagcccga gaaaagaatc gcgagaatcg
     2401 gtttgcgcca cgaagccagt gggttccagt tgagccagcg aacggtattg agcccggcga
     2461 acgccaggat ggccgcagcg gccgcgtagc gtaaactgtc gtgcagcggt agtgccgcgt
     2521 acacggcgat caaaaacagg gtcatcggaa aaatgctgtt ctccaggcgc tgcgaaatac
     2581 gaggcttcgc ctcgggtatc gccagcgagg tgaaaaaagg cagaattcgc cccgcgatga
     2641 tgacaatgaa tgccagcacg acgagaagcc ccatgtgaat gctatgctgc acagggatca
     2701 atgcctgggg tgtaagcagg gcgataaggg caaggagctg cacagcgatg aagataccat
     2761 gcaggtagac caccttgtgg ttgtgtttct gcacgggctg cagaaggggt ttcattcgcg
     2821 tgagtgcgaa agctccccct aagatatcga tgcctgcgcc gaagtaaacc agcggagcgg
     2881 gtaagaacca ggcgaccctg ccgagtagcc agctggtaaa aacgatcgcg agtggtccgc
     2941 gcgtcagcat ctgctggccg caccagtttt gcccggcggt aaaaaggaag cccaggatcg
     3001 cggcccggca gaaaccgaag accatttcgt agctatgcca ccacgtgccc gcaatagccg
     3061 tcggcagaat atcactacct gtcaatttgg cgagccagac agaaatgata aagagtgcat
     3121 gcaatccggt tgcgagaaaa aaaacccgga aggcggtggc aaaaaccggc cagtgaaaca
     3181 tacctgcaag gtggggcagg tcaaggccat cgcccttgat caggatcaat tatgggcgaa
     3241 ttttctctct aaaattgtca acgctcacac tgagaccggc gtgctgcaga ataatttccc
     3301 cctgcggtga aagcaggcta aaaagcgatg aatgggcaaa ttcgccgtca tcctgctttt
     3361 tgtaattaac tcccagcgca gcagctacct cgcgaatgct ttcggctttg cccgttagca
     3421 gcacgaacga atcggtcagt ttcatcttgg ctataaactt ctgcaggttg gcgggggagt
     3481 cacggtcagg gtcaaacgaa atcatcagga aggttgtgtt ggcgcgctgc gccgatgaca
     3541 ggttcttctc gagcctgagc atctcggctg cgatacgcgg gcaaatcgct gtacaagtgg
     3601 cgtaaaacat ggccacgacg acatacttgc cctgcagctg gtgcagcatt aattgtctgc
     3661 cgttctggtc tgtgaacatg tcgtgaaact ggtagagtga agcacctgaa actttacctg
     3721 cctccatctt gtggtgcgcg tgcggatcga cttctttttt gcatctgatt accgcattcg
     3781 caagagcaaa catgctaatg agtatgatca ttcttttcat tatttatccc tggcacaccg
     3841 gaagccgaga ttattgccgg tgtagtttcc cttgagagag ctgcgcatgc catagcgcat
     3901 gtaggttgca tagttgcgca aatcactgcc tgaaagcgac gtggcaccgc agaagaggtt
     3961 cgattcggta tcggtgtccg cgcgtgaatc accggtgacg gtagacgaat tgaaatcatc
     4021 gacccattcc cagatgaggc catgcaggtc gtgtacgcct tgcgggctca cgagccctga
     4081 gccgatagtt ccgccggtcg tctggtcgtt cggtttaccg aaccaggcaa gaatcctgct
     4141 gttcgcgtcg ctgcgctggt cccacttgtt aaaggtggct acatattccc attcatacaa
     4201 cgtcggcagg cgtttgcctt gcgccgcgca gtatgcccgc gccgcgtacc atgaaacctg
     4261 tgtcatgacg acgctgttcg atggttgtga aagattaatt gttttgagat aaccctggtc
     4321 ggccttgatg gggggagcgt tacccctctg ccaggccggg tgtgccgcaa caaaagccgc
     4381 gtactgggca acactgaccg ggtatttatc cagcacgaac gatggaactt tgaccggctt
     4441 ctgctctttg cgcaggtcaa agggacgaaa actgcccgct ggaatgcgca ccatcgtgcc
     4501 atcaccagcg gacagggcag cccagctaag aaccaggctg caaattgtca ggttagactt
     4561 cagtgcacgc tccgctgagc ggcaacttcc tgtggcgtca ctacgccgcc cgagttgccc
     4621 catgaattga gcacgaagct gagtacgttt gcgacgtctt cgtcgctgag gcccagtgca
     4681 ggcatcacgc tgtcgtattt ttctttattc acgacgatcg gaccactctt gccttcgagg
     4741 actactttta ttgaacggac tttgtccgcg ttcaggtagt cagacttcgc caaaggtgga
     4801 aatgccttgg gtactccctg gccttcgttc atgtggcatg cctggcaggt ggtgccatag
     4861 atgcgtttgc cttcggcgat gcgctcttct ttagatttgg cgacaacctt catgtctttt
     4921 tctgcgggca tcgtctgtgt cgcgccacct tcgccgaggt agacagtatc agcctgtttg
     4981 cccgagtaga tcgcggtgtt ttcagggcct tcagctttca gaatgcccag cgcccccttg
     5041 ttgaaggtac ggaagattga gtggtcaacg atcacgtagt tgccgggtac atccatcttg
     5101 aagtcgacga tcgcagaacc gccagcggga acgagagtcg tctgcacgtt tttctggttc
     5161 ttctccgtac caccttcggt atagacgtgg tcgaagattt cgccgatgac gtgaaacgac
     5221 gacacaaggt tcgggccacc gttgccgaca tagagacgca cggtttcgcc gacttttgtt
     5281 ttgagtgcct tgtcaccggt cattgaaccg acagaaccgt tgaagaccac gtattcaggc
     5341 cgttcgtcga gcgccttgtt catatcgaac ggttgaagac cggcctggcc gtttttgccc
     5401 ttggtataga actctgactg catcacgtag aattctttgt cgactttggg cattccacct
     5461 ttgggctcga caaggatgag gccgtacatg ccgttcgcga tgtgcatacc cacgggcgcg
     5521 gtcgcgcagt ggtagacata gaggccggga tttattgcct tgaacgagaa tttcgaactg
     5581 tgaccgggcg aggtcagcga ggctgccgca ccaccgccgg ggccggtcac ggcgtgcaga
     5641 tcaatgttat gcggcatctt gctcgttggg tggttcgaaa gcgtgaattc gacatcgtcg
     5701 ccctcgcgca cgcgaatcat cgggccgggt accgtgccgc cgaatgtcca aaacgtgtac
     5761 tctacaccgt cggcaaggcg ccccttgagc tcttttgttt cgagttcaac gatcaccttg
     5821 gcgttgccgc tgcggtcgat cggttttggg acctcaggcg catgcgtcag aactgccttt
     5881 tcggtctttg taccacaggc aatgaaggtt ggtaccgcca gcgccagcag gaacagcgtc
     5941 gctgccccgt gagagagtgt ttttttcgat ttcataaatc ctcctggaat ctgtcatgat
     6001 tatagaccgg aaactccatt ttgtcgttga tatggatcaa gagaccaaca atttatttgt
     6061 aattggcgac ggccgggccg ggcaggttga tctggatcaa ttttgatttg tttcctgatt
     6121 catctttgtg ctgtggttca gaaatgcccc ggttttgctg cccatgaaca caatcgactt
     6181 tggtgatctt gcccctacca gcgcagataa attgcgcgct attgcgcatt accggcgctt
     6241 tagccgcaac gaagcggtgt ttcgcgaggg tgatcgttat gcgggaccgt tcctggtcac
     6301 tgaagggcaa ttcaaaatct atatgcttgg cgatgagggc aaagaagcca tcatgcatat
     6361 cttcaggccg ggcgagctga ttgcgggcgg tccgcttttt ctcgggggta attatccggc
     6421 ctattgtgcc gcgctgtccg atggatgcct ggtggctttc gagtatgacc gcctgaagaa
     6481 gttacttgtg acagatgatg ccgtgaatgg ttttttcgtg ggcaagagcg tcaggttgat
     6541 accacgttta aaagataaga ttgagaatct cacgctgaag aatgccgagg ggcgaattta
     6601 cgcttacttg aaatcgcttg gggcagacaa ggcaccgata gaacttgata tacccaagaa
     6661 ccaaattgcg gcgctgctcg atctcacccc cgagtcagtc agcagaatct gtaaccagct
     6721 gatcagtaaa ggcatactgg tggttgaggg caagacgtac cgcctgcagc cttaagaccg
     6781 taatgccggt tgagtaacgg gctatgattc tttccaaact tcgggagtta tggttgtttc
     6841 gcgcccatga tccgaaaaag aaatatctcc gtcctggatc atacactgca gttgcatggt
     6901 gcgctctgac ataccggaca agggcctgac ctggtcatcg ggtaagaact tcacgcacag
     6961 gttgtcaaag cggtcgagcg atgctttgat ggattgccac cacggtttcg cgcggtgcgc
     7021 gccatagcaa tagacatata ctttgcgcgc gcggccacat gctttgcgta tacgcttctc
     7081 gtccggctgc ccgatttcga tccattccag aatttcgccg gagagatttt tgcgccagag
     7141 cgcgggagtg tcttcggtac tgatgccccc tgcgaattca agggctgggt cggcgtgtat
     7201 ggcgaagagc agcaggcgca gcatcatccg ttcgtctgtt tctgaaggat gccgcgcgag
     7261 cgtcagctta tgcgactggt agtagtcgcg gtcggtatcg gtcacctgca ggtcggcctt
     7321 aaatatggtg gattttattg ccacgggccg tacgcaaccg gctgccatct ctttgcgtaa
     7381 agaggttttg ccgggtgtct ttaaaagatt gcaggcgaac gatcgagttc gcgtaagacc
     7441 tggccataag cttgcgaaat ttcgtcctcg gaaaacaggt ggtgtttctt cttctctaca
     7501 ccatattccg aaagaacaaa gtgccgcttt gccacgacac cgtggtgtgc gaggcagtgt
     7561 tcaacgcact tcagcgcgca gccatcgagc gcgatgatcg gccggccgct tttcgccgtg
     7621 cgcaccagcg gcttcacacc gccgccgaca ccggcaatac acgacatctc ggcgtggcct
     7681 tcgcggtcga ggcggagcgc gatggaattg gccatctgtg ctgccgaaga gcagcctgaa
     7741 cacgaataaa cgagcggcag ctcaggttgc atgcggcctc cgggccagca gaaagagggt
     7801 cgcggtaaaa agaacaagtg cattcgcctg gtggagtgca cccagcgaaa caggtactgc
     7861 cttcagcagt gtaaaaacac cgagcacaaa ctgcagcatg tatgccatga caattaacgc
     7921 cagcgggtgc cccagggaga tatttttacg ttcgcgccag agctgccaga aaaagaaaat
     7981 gccgagtgca taggcgaagt tgcggtgtat gaactggatc atgattgggt tgtcagaaaa
     8041 attcgataga accggggaaa atcccagcgc gccttgcgga aagtactgcc cgttcatgag
     8101 cggaaacgtg ttgtacatct tacccgcctt cagcccggcg acgagtgcac cgctggccat
     8161 catgacaaag acgaggataa catttgccca ggcgagccgt cgcgagccgg gaaggccggg
     8221 ccgtttttcg aggctccatg cggtcatcat catcagcgca taaataagca ccgcggtcat
     8281 gaggtggctg gtcagccgca ggtggctgac atgcgggtcg ttcaccagcc cgctcttcac
     8341 catgtaccag ccgagaaaac cctgtaagcc tcctagcgcg aagatgacac cgagcctgag
     8401 tgcgagcgtc cggtcgatgc ggcggcgtac cagaaaccag ataaacggga ggaagaatag
     8461 taccccgacg aggcgtgcga agatgcggtg gatgaactcc caccagaaga tgttcttgaa
     8521 ctcgggcagc gtcatgctgt ggttcacgag cctgtattcg ggggttgcct tgtatttttc
     8581 gaactcggcc agccattcgg tttcacccat gggtggtaca acgccgctca ccggtttcca
     8641 ctcaacgatc gacaggcccg aacgcgtgag acgggtgatg ccgccaatca cgacgatggc
     8701 aaaaacgact gctgcgcaaa cgaagagcca gtttgtgatg gcccggtggg attcaggtct
     8761 tgtcgcttgc atcgtctata agtctcctta tcgaccgcca ctcttcgggc attttttccc
     8821 cttcggtcag cagaatggct ttgtcggaat cgtcggccat gtcacggcgg tgcagtatct
     8881 cttcgctctg ttctgccagc tggtgcaggg gacgcagaat ccagagccag acaggtagaa
     8941 gcactaagag cacacagaca accagcaggc cgaagttctc ccagaacaga tcgagggtaa
     9001 aatagctgat acgcgaatcg gtgatcgctg tgagaggtag cctgcgaata gcgatgccca
     9061 ctggctggcc cagcgttata ggcttaccgt ctgcgccgac atggcaggtt tggcaaactg
     9121 gctgcgcctt gagcgggtaa gcgtattcga tgaactgctc cgtctggcgg aacgcagctt
     9181 cagccgtgta catgacacgc ttttcaaagt cgttttgcgg ctggcggtcc tggcgcttac
     9241 cgaactggct gtcgagaaac tcgctgcgcc tcagttcgat cggaatcgtt ttatcggcgc
     9301 tgatggcgta cagggcacgc ctgacgtatt ctttgtcgcc gttggccgca gtgaccacga
     9361 cgccgatgat tttctcgaat gcctgcattt cacggtgaat ctgttcttcg accgtgaggc
     9421 gggtaaatcg ctctgctgcc gggagaaaat acagcttgat caccagcgag gcgagcgtga
     9481 gaaccgtggt ggccaaaatc cagatgcggg tagagattct catgtcagcg tgttgctccc
     9541 ttcgttttcg tcatcgtcct tgaaaccgcc ggcgagccag aacgcgccaa aaattactcc
     9601 gattaccgaa ccaaaaaaca gcactgacat gaaactcatt gccatcagcg ccgaaaacat
     9661 gttgcgcgcg cggcgcatgc catagtcaaa ctcggtttca cccggctgct tctcgcctgt
     9721 ccagccacag aattcctgca gtgaggagta gctgataatt tcaccacggg ttttggcgcg
     9781 catctcaaaa tagaagtctt ttatgccatt cgcagcactc agaccccata gtccaatcag
     9841 aatggagata aaggtaccct tgttgcgcct gatcataagc ttttttttac ctcgtc
//
LOCUS       DAOQJF010000089         9811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3650_length_9811_cov_5.464227, whole genome shotgun sequence.
ACCESSION   DAOQJF010000089 DAOQJF010000000
VERSION     DAOQJF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3650_length_9811_cov_5.464227"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..448
                     /locus_tag="PLF85_06435"
     CDS             92..448
                     /locus_tag="PLF85_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNE19336.1"
                     /translation="MRHYAWRRDHYQTLQEFWPFYLHEHSSARNRMLHFVGSTLGLVI
                     FAVALGLSNYVLVVPALVSGYAFAWFGHFFIENNRPATFKYPLKSFLSDWRMWYTMLT
                     GRIAVELGKFGIESRP"
     gene            515..2317
                     /locus_tag="PLF85_06440"
     CDS             515..2317
                     /locus_tag="PLF85_06440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19337.1"
                     /translation="MLELKYGKRRVVKYRGAKTVIGGLTPKDKIDILMYVSKELSTIN
                     DTQLLFDRVIELCSEIFEADNVTVRIEEAGRLNPVAFLRQTTPERRPLAVGEGFSGSI
                     FTSQQSRLIYDLAQHPEYLDPGEETLCVICAPISVREEKLGTLAVEKNTSHFYKRDDL
                     EILEAMAAQIALAILNARLITGMLDAQKVQKRMNSQLQFDLRIGRTVQSQIIHSDIPS
                     FRAIDIKAAYEPMVEVSGDYFDYYRSHESLTVIIADVSGHGVPAALITMALHYHFRLL
                     SAQGLSLLELLSQLNEHMKPILPSGIYFTAQLLRIHSDYSFTFVNAGHPKMLHFSAMS
                     SELLAQYDTPGFPLGIAPFDVREYEEVSGKLRPGETLVLLTDGFAEQQNSSHEQPGAE
                     KVVSWLRAALSEPSQAIEAFMQSFKLYRGDEPNGDDLTVLTLHYNLRHLEAETMVYQS
                     RSRMRSADYAGARQLALSALAVRPQHQDALIIVGKLALLQRDFRTASQYLAQACALDD
                     HVDPQILLLAGHAAYKAEDISSAKRFLKLLLSEEPQNIKAALLLIRCYVRAREPHKAT
                     RTLNAALKFAPQNEKLQQVADFLRKKDMPVPAAV"
     gene            2387..3127
                     /gene="raiA"
                     /locus_tag="PLF85_06445"
     CDS             2387..3127
                     /gene="raiA"
                     /locus_tag="PLF85_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNE19338.1"
                     /translation="MEIAYYFKNLPPTDAIKQYASKKIDKLKERLHHIEGIDVRFSLE
                     RQNQFFEITVHGDATVFHLKKSDKDLYAAIDNALDVLNNQIDKYRKKLEEKTAVAREV
                     FLPKTDERVVSEETEIDVREAEIKPMDDIEAILQLRSKKFRFLMYHHADTIRYGLVTA
                     RNDGNYSIVMPAADGFTETVVRLQDNKLEKLAEAVYPVSKFTVAEALDVLNEANLEFL
                     AFVNEETGRLNVIFHANGGGIMIKRPAA"
     gene            3184..4062
                     /gene="hprK"
                     /locus_tag="PLF85_06450"
     CDS             3184..4062
                     /gene="hprK"
                     /locus_tag="PLF85_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNE19339.1"
                     /translation="MNEDADLDRELREIDVNRPGLALNGFFEHFAHDRMQVFGQGEHA
                     YVSSMNPERISEPLERYFNYPITGIVFTHDNYPPEQFLSYAVKNRTPVFVSSLRTSKF
                     INVFTDFLDAALAPQTLLHGTLVEVFGVGVLIQGKSGVGKSETALELVERGHRLVADD
                     AVRIVCRDGSRLQGFVSETIGHHLEIRGLGIINISDLFGAGAVRNDKLIDLIVHLEDW
                     DDKKEYDRLGLEDVTEDILGVDIAVMTIPVRPGRNVPVLIETAAKNFNLKSRGHHAAR
                     KFQERLARMIREAAES"
     gene            4071..4337
                     /locus_tag="PLF85_06455"
     CDS             4071..4337
                     /locus_tag="PLF85_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNE19340.1"
                     /translation="MVSKTVTIQNETGVHARPAAVFVKMANRFPCEIFVEKDEQRING
                     KSIMGLMMLALTRDSRITVIAEGDKEADAVEALCRLVENSFVEA"
     gene            complement(4372..5310)
                     /locus_tag="PLF85_06460"
     CDS             complement(4372..5310)
                     /locus_tag="PLF85_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE19341.1"
                     /translation="MVLKKSAANPADRTLSASERPFLIAEFGLNHNRDIALAMKMADA
                     AAASGVDAVKLQSYTTRFFINRQFENVHGLYDIFAGLELDAEFHRGLRDYVVSKGMAF
                     FSTPLTEDWVTTLEALEVPAFKIASGDVNNFGLISAAAQTGKPLLVSTGAASEEEIRR
                     AIKRLEDLGGSYCLLHCVSLYPTPLAKANIGRLARIRQLLPDPANIPLGFSDHTEESD
                     AAFAAIAAGAVVIEKHFTLDKTLPGPDQKMSSDPAEMRELRRRIDLAWSIRGTAQNAD
                     CHVEETAGDYYGKRAIYEFEGRKLAMRPRHPEYPRP"
     gene            complement(5359..6609)
                     /locus_tag="PLF85_06465"
     CDS             complement(5359..6609)
                     /locus_tag="PLF85_06465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE19342.1"
                     /translation="MLNSSARKNPKKDFFTLKPEEAKSAAPLSASLPNHTTGTQSATP
                     LRINLGFAEISYQGKGQFLYFYKRGGTLKSGALRVDRKAALWMVMGVICLSASLGLMF
                     WKKRQNAVKLAFAETKTGVGGVGRLESGKVINDAADITGEIAQFEEDEKIKNQLMGVI
                     EERSEESKAAEKQANKVQKLQYKVKPGDTLQSIAKKYKVKPESISGSSRLDEFQDVSV
                     GQTLSIPTRDGIYYIVKKNERLANVLQAHKVSLDKFIAENEHVNVDLLDAGEEIFLPG
                     AKPIRRAEGWLIPVSSRVVTSGYGWRSWPRSAFHKGLDLKAFYVPVRAAKSGTITYSG
                     WLGGYGNAIIIQHDDTYKTLYAHLSRRHVAVGQRVRRGQVLGRTGDTGYSFGPHLHFE
                     ISKNGENVNPIKFLKGLRGRSKKR"
     gene            complement(6690..7097)
                     /locus_tag="PLF85_06470"
     CDS             complement(6690..7097)
                     /locus_tag="PLF85_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE19343.1"
                     /translation="MAKYDNTNSIIGEGSIFEGRFYIAGSLRVDGKFEGDIRTDEALV
                     VGETGKVKTNISAREVILAGTLIGDIEAKDEVRLTETGRMLGDITTPVLNISRGVVLK
                     GNVNIHGGHKKDPRKIVEESYGLVRELDKKGIQ"
     gene            7280..7813
                     /locus_tag="PLF85_06475"
     CDS             7280..7813
                     /locus_tag="PLF85_06475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE19344.1"
                     /translation="MLLKGKAVLLLATACICSATEALRHHAERADQPDFADYRQSGRV
                     IRTYSGNAEHNHYGVTLRLRDASVRALAEGTVYSAGQLRGYGNVVIVDHGHGWHTLYS
                     DIAEIRVAVGQQVRRGAVLGTPREKRLFLVVSYKGNPINPSDVIGRRPRNAGGHSERV
                     QSRSGVPSLAAVAINAG"
     gene            7817..9049
                     /locus_tag="PLF85_06480"
     CDS             7817..9049
                     /locus_tag="PLF85_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE19345.1"
                     /translation="MAWELVNGTIVTPRDVIERGSVQIEKTRVSQVSKSSRKSADMMQ
                     IDVNGMLIFPGLINCHDHLLGTYVPRVGDRRPYLNWLEWDNDLKSSPIYAERQQIESS
                     DLYQLGGFRHLLCGITRVQDHIPHFVRELFAKDMPIRIISDFAMAHSVTSFALKWGDG
                     IEAEHAKAKEKNIPFITHCSEGYDEETLRSVETLAAKKALSKQTVLIHGIAFTDKDME
                     LLAEHQCNVVWCPVSNLYMFEQTARVKELMDMGINVTLGTDSPMSGSFSIFEEFRTAR
                     EFYQYAYGRAIDDRALVDMMTVNAARAMCLPDAGEIAEGKAADLLLIDGNSTNPWAAL
                     THADYADIMLVIAEGQPRYGDIAFAPVFDAMKIPYQTILVKGARKIIAGDILGLQNRV
                     RRAVKFRKELDFLPIEAA"
     gene            9049..>9811
                     /locus_tag="PLF85_06485"
     CDS             9049..>9811
                     /locus_tag="PLF85_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE19346.1"
                     /translation="MPLTRNYSAGSIVYFAGDVGEDIYVMQKGRISLISTSLDQKDDI
                     KEDVRKGEFFGVKSALGRYPREETAQVLADSQVLVFKLAEFEQFAQKNTRIVLQMLKV
                     FSSQLRRVHKAVREFLGEHGASEASMELVRVGEYYYKAGKTDYALYVFNAYLKNYPAG
                     SLTERVRRLAQLVKEGAPFPIDLPDINEEVHSNDVAQRTGQIAQQMHDDPADDYAIHD
                     HGDITPDLPDMGDMPGDLPDLPPPAGDFDMPMPDDF"
BASE COUNT         2221 a         2644 c         2700 g         2246 t
ORIGIN      
        1 aaacaagagc accgcagcga gaggtgtctc tggcgtggcc cgccgcgccg cggagcaggt
       61 gcaactggcg gccgagcgca gccgcggtgg aatacggcac tacgcctggc ggcgtgacca
      121 ctaccagacc ctgcaggaat tctggccctt ttacctgcac gaacactctt ctgcgagaaa
      181 ccgcatgctg catttcgtcg gttcgacgct aggacttgtg attttcgcag tggctttggg
      241 attaagtaac tatgtcctgg ttgtaccggc actggtgagt ggttatgcct ttgcctggtt
      301 cggtcacttt ttcatcgaga ataatcgacc tgcaaccttt aaatatccgc tgaaatcctt
      361 tctctctgac tggcgtatgt ggtacaccat gcttaccggg cgaatcgcag ttgagctggg
      421 aaaattcggt atcgagtccc gcccgtaacg gccagcagca cacaagttct gcgcgctatt
      481 cgttgtggca tgagttttgc tgcagcgggg caggatgctc gaactcaagt acggtaaacg
      541 gcgggttgtc aagtatcgcg gggcaaaaac cgtcattggt ggtctgacac ccaaagacaa
      601 gattgatatt ctgatgtatg tcagcaaaga gctgtctact atcaatgata cccagcttct
      661 ttttgatcgt gtgatcgagc tctgctcgga gatttttgag gctgataacg ttaccgtacg
      721 catagaagaa gcgggtcggc tcaacccggt tgcgttctta cgccaaacga cccccgagcg
      781 caggccactc gcagtgggcg agggtttttc cggttcgatt ttcaccagcc agcagtcgcg
      841 gctcatctac gatcttgcac aacaccccga atacctcgac ccgggtgaag aaacattgtg
      901 cgtcatctgc gcccccatca gtgtgcgtga agaaaaactg ggcaccctcg cagtcgagaa
      961 aaatacttcg cacttctaca agcgcgatga tctcgagatc ctcgaagcca tggctgccca
     1021 gatcgcgctg gcaatactca acgcgcgact gataacaggt atgctagacg cgcagaaggt
     1081 gcagaagcgc atgaattcgc agttgcagtt cgacctgcgc atcggccgta ctgtacagtc
     1141 gcagattatc cattcggata taccgtcctt cagggcaatc gacattaaag ccgcgtacga
     1201 gccaatggtc gaggtcagcg gcgactattt cgactattac cgctcgcatg agtcgctgac
     1261 cgtgatcatc gccgatgtat ccgggcatgg ggtaccagcc gcgctgatta ccatggcgct
     1321 gcattaccac ttcaggttgc ttagcgcaca ggggttgagc ctgctcgagc tgttgtcaca
     1381 gctcaatgag catatgaaac ccattctgcc ctcgggtatc tattttaccg cccagctgct
     1441 gcgcattcac agcgactaca gcttcacctt tgtaaacgcg ggacatccca agatgctgca
     1501 tttttctgcc atgagcagtg aactgctggc gcaatacgac acaccggggt ttccgctggg
     1561 cattgcgccg tttgatgtac gcgaatatga agaggtgagt ggaaagctgc gtccgggtga
     1621 aacgctggtg ctgctgaccg acggtttcgc ggagcagcaa aatagcagcc acgaacagcc
     1681 gggggcggag aaagtcgtat cctggctgcg cgctgcgctg tctgaacctt cccaggcgat
     1741 tgaagcattc atgcagtctt tcaagctcta ccgcggggac gagccaaacg gcgatgacct
     1801 cactgtgctc accctgcatt acaacctgag gcacctcgag gccgaaacca tggtatacca
     1861 gagccgcagt cgcatgcgct ccgccgatta cgccggcgca cggcagctgg cgctttcggc
     1921 gctcgccgtg cgaccacagc accaggacgc cctgattatt gtgggtaaac tggccctgtt
     1981 gcagcgcgac ttccgtacgg cgtcgcaata cctggcacag gcctgcgcgc tggatgacca
     2041 tgtcgatccg caaattttgc tgctggcggg gcatgccgcg tacaaggcag aagatatttc
     2101 atcggcaaaa cgctttctca agctgctctt atcggaagaa ccccagaaca ttaaggctgc
     2161 attgctgctc attcgctgtt acgtgcgggc ccgcgaaccg cacaaggcta cgcgcacact
     2221 caatgctgcg cttaagtttg caccgcaaaa cgaaaaattg cagcaggtgg cggattttct
     2281 gcgaaaaaaa gacatgcctg ttccggccgc agtgtagcac cgctcagcac cttcggaaaa
     2341 aacaaaaaaa cgctcgatca cttctgtgag ggtttattat cgccttatgg aaatagcata
     2401 ctactttaag aaccttccgc ccactgacgc catcaagcag tatgcgtcaa agaagatcga
     2461 taagctgaag gagcgcctgc accacatcga gggcatcgat gtacgctttt ctctcgaacg
     2521 ccagaaccag ttctttgaaa ttaccgttca cggcgacgca accgttttcc acctgaaaaa
     2581 aagcgacaag gatctctatg cggctattga taatgcactc gatgtcctta acaaccagat
     2641 tgacaagtac cggaaaaaac tggaagagaa gaccgcggtc gcgcgcgagg tttttctgcc
     2701 caaaactgac gagcgtgtag tctctgaaga aactgagatc gacgtgcgcg aagcagagat
     2761 caagccaatg gatgatatcg aggcgatcct ccagttgcgc agcaagaagt tccgttttct
     2821 catgtaccac cacgcggata caatccgtta cggcctggtc acagcccgca atgacggaaa
     2881 ttacagcatt gtcatgccgg ctgccgacgg ctttaccgag acggttgtcc gcctgcagga
     2941 taataagctc gagaagctgg cagaggcagt ataccccgtt tcgaaattta ctgtcgccga
     3001 agcgcttgat gtactgaacg aagcgaacct cgaatttctt gccttcgtca acgaagagac
     3061 cggccgccta aacgtgatat ttcatgcaaa tggtggcggt atcatgatca agaggcctgc
     3121 cgcctgaagt ctgtaaaggt ccgcgagctg ctggggagcg ctgatcggct gaagatcagg
     3181 ctcatcaacg aagatgcgga tctcgatcgc gagcttcgcg aaatcgacgt caaccgtccc
     3241 ggtcttgcgc tcaatggttt ttttgaacac tttgcgcacg accggatgca ggtctttggc
     3301 cagggggagc atgcttatgt aagcagtatg aaccccgagc gcatcagcga gcccctggaa
     3361 cgttatttta attaccctat aacggggatc gtcttcactc acgacaacta tccgcctgag
     3421 cagtttctgt cttacgcggt gaagaaccgc actccggttt ttgtttccag cctgcgaacc
     3481 agcaagttca tcaatgtttt cacggacttc ctcgacgccg ctctggcgcc gcagacgcta
     3541 ctgcacggca cgctggtcga agtctttggt gtcggtgttt tgatccaggg aaaaagcgga
     3601 gttggtaaga gcgaaaccgc gcttgaactg gtcgagcgcg ggcaccgact ggttgcagat
     3661 gatgccgtgc gtatcgtttg ccgcgacggg tcgcggctgc agggttttgt ctctgagaca
     3721 attgggcatc acctcgaaat acgtggcctg ggaatcatca atatcagcga cctctttggt
     3781 gctggtgctg tgcgtaacga caagcttatt gacttaattg ttcatctcga agactgggac
     3841 gataagaaag aatacgatcg cctggggctc gaagatgtga ctgaagatat ccttggggtt
     3901 gatatcgccg tcatgacgat accggtccgt cccggacgaa atgtacctgt actgattgaa
     3961 actgcggcca agaatttcaa cctgaagtcc cgcggtcacc acgcggccag gaaattccag
     4021 gaaaggcttg cacgcatgat tcgcgaggca gcagagagct aaaagacgcc atggtttcca
     4081 aaacagtcac gatccagaat gagacaggtg ttcatgcccg accggcggct gtcttcgtca
     4141 aaatggccaa ccggtttccc tgcgagatct tcgtcgaaaa agacgagcag cgtatcaatg
     4201 gtaagagtat catgggcctg atgatgcttg ccctgacccg ggacagccgc ataactgtaa
     4261 tcgccgaagg cgataaagaa gccgatgcag tcgaggcgct ctgccggtta gtagaaaaca
     4321 gttttgttga agcttaagag cccctaagcg acagataacc gcgaggccgt ttcacggcct
     4381 gggatattcc gggtgtcgcg gacgcatggc cagctttcgg ccctcaaatt cgtagattgc
     4441 ccgtttgccg tagtaatcgc ccgcagtctc ttccacatgg cagtcagcgt tctgcgcagt
     4501 acccctgatg gaccaggcaa ggtcaatgcg gcgccgcagt tcacgcattt cagccgggtc
     4561 tgaagacatc ttctggtcgg ggccgggcag ggtcttgtcg agtgtgaagt gtttttcaat
     4621 gactacagct cccgccgcga ttgctgcgaa agccgcatcg ctttcttcgg tgtggtcgga
     4681 aaaaccaagg ggtatatttg ccgggtcggg tagcagctgc ctgattcgtg ccaggcggcc
     4741 gatattggcc tttgccagtg gggtgggata aagcgaaaca cagtgcaaca ggcagtagct
     4801 gccgccaaga tcttccagtc gctttatggc gcggcggatt tcctcttctg atgctgcacc
     4861 ggttgatacc agcaggggtt ttcccgtttg cgccgcggca gaaataagcc cgaaattatt
     4921 cacgtcgccc gaggcaatct tgaacgctgg tacctcgagt gcttcgagcg ttgtaaccca
     4981 gtcttcggta agcggtgtcg agaaaaatgc catacctttg ctgacgacgt aatccctcag
     5041 cccgcgatgg aactccgcat caagttcaag gcccgcgaag atatcataga gcccgtgcac
     5101 attttcaaat tgccggttga taaaaaaacg tgtggtgtac gactgcagct tcaccgcgtc
     5161 gaccccactt gccgccgcag catctgccat tttcatcgcc agggcgatat cgcggttgtg
     5221 gtttagtccg aattctgcga tcaggaatgg gcgttcagag gcagaaagag tgcggtccgc
     5281 cgggttggcg gcagatttct tcagtaccat agaagtttat cggcgtgccg gaaaacgagg
     5341 aagaccgaaa aacgctaatc aacgtttttt tgaacgaccg cgcagacctt tgaggaattt
     5401 gatcgggtta acgttttcgc cgttcttgga aatctcaaaa tgcaggtgtg gcccaaacga
     5461 gtagccggtg tcaccggtgc ggccgagaac ctggccgcga cgcacccttt ggcctactgc
     5521 aacatggcga cggctcaggt gcgcatagag tgtcttatag gtatcgtcgt gctgtatgat
     5581 gatggcattg ccataaccgc ccagccagcc tgagtaggtg atggtccccg atttggccgc
     5641 acgcaccggc acatagaagg ccttgagatc tagtcctttg tggaaagcag agcgtggcca
     5701 gctgcgccag ccgtaaccac tggtgacgac ccggctggaa accggtatca gccaaccctc
     5761 ggcgcggcgt atcggcttcg cacctggcag aaaaatttct tcgccggcat cgagcaggtc
     5821 gacgttcacg tgctcgtttt cagcgatgaa tttatcgagt gaaaccttgt gtgcctgcag
     5881 gacgttcgcg aggcgttcat tctttttcac gatgtagtaa attccgtcgc gtgtgggaat
     5941 agaaagcgtc tgtccgacag acacatcctg gaactcatca agccggcttg agccgctgat
     6001 tgactcgggt tttaccttgt atttttttgc aatggactgc agggtgtcgc cgggtttcac
     6061 cttgtactgc agtttctgga ccttgtttgc ctgtttctcg gcggccttac tttcttcgga
     6121 gcgttcttca atgacgccca tcaattggtt cttgatcttt tcatcttctt cgaactgggc
     6181 aatttcgccg gttatgtcag cggcatcgtt gatgaccttg cccgattcga ggcgacccac
     6241 accacccacg ccggttttgg tttcggcaaa tgcgagcttc actgcattct ggcgtttttt
     6301 ccagaacatg agcccgagcg aggcgctgag acagataaca cccatgacca tccacagcgc
     6361 ggctttgcgg tcaacgcgaa gcgcaccgct cttcagcgta ccgccacgct tgtagaaata
     6421 cagaaactgt cccttaccct ggtaagagat ctcggcgaaa ccgagattga tacgcagggg
     6481 agttgcgctt tgtgtgccag ttgtatggtt tggcagcgac gcagaaagcg gcgcagcgct
     6541 tttggcctct tcaggcttta gggtgaagaa atctttcttc gggtttttgc gagccgatga
     6601 attaagcatg ccaggcctgt ttccatattt tcgacccgga gcacccgttt gtgtgagacc
     6661 tttttttctg gccaagacgc caacccgttt tattgaatgc ctttcttgtc cagctcacgg
     6721 acaagaccgt agctttcttc tacaattttg cgtgggtctt ttttatgacc accatggatg
     6781 ttaacattgc ccttcagtac aacaccacgt gaaatgttga gcaccggggt cgtgatgtcg
     6841 ccgagcattc tgccggtttc ggtcagccgt acctcatctt ttgcttcgat gtcgccgatc
     6901 agcgtgccag ccagaatgac ttcgcgcgct gaaatattcg ttttgacctt accggtctcg
     6961 ccgaccacca gtgcttcgtc cgtgcggatg tcaccttcga acttgccgtc tacgcgcagg
     7021 ctgccggcaa tgtagaagcg accttcgaaa atcgaacctt cgccgattat agagttagta
     7081 ttatcgtatt tagccatagg tcacctgtgc ggaaaatatg ggtcagaatc aactaatgag
     7141 gcgcggcgag aaccaaaaaa ggctttcagg agttcgtgcc cggtataagg gtgaaggcgg
     7201 ggcgggctgt attacaggcc gaggtgccgt ctggcggcaa aactcaaaat cgttctgttt
     7261 tttgccgata tacagacaga tgctcctcaa aggaaaggcg gttctcttgc tggccactgc
     7321 ctgcatctgc agtgcgaccg aagccttgag gcaccatgcc gaacgtgccg atcagcctga
     7381 tttcgccgat taccggcaga gtggtcgcgt catcaggacg tattccggca acgcagagca
     7441 caaccactac ggtgtcactc tgcgtctgcg cgacgccagt gtgagagccc tggcagaggg
     7501 gacagtctac agcgcaggcc agctcagggg atatggcaat gtcgtcattg tcgaccacgg
     7561 tcacggctgg cataccctgt attccgacat agccgagatt agggttgcgg tcggacagca
     7621 ggtcaggcgc ggcgcggtgc tcggtacgcc gcgcgagaaa aggctttttt tggttgtatc
     7681 ctacaagggc aatccaataa acccctcaga tgtaattggt cgacgccccc gcaatgccgg
     7741 tggtcattca gaacgagtgc aaagccgttc gggtgttccg tcgctggcgg ctgtcgcaat
     7801 aaatgcagga taataaatgg cttgggaact ggtcaacgga acgattgtaa ctccgcgcga
     7861 cgtgattgaa cgtggttcgg tgcagattga gaagacacgt gtctcccagg tgagcaaaag
     7921 ttcgcgcaaa tctgcggaca tgatgcagat cgatgtcaac gggatgctga tttttcccgg
     7981 cctcatcaat tgccacgacc acctgctcgg cacctatgtg ccacgcgtcg gcgaccgcag
     8041 gccgtacctc aactggctcg aatgggacaa cgatctcaag agttcgccaa tctacgcgga
     8101 gcggcagcag attgaatctt ctgacctgta ccagctgggt ggttttcgcc acctgctctg
     8161 tggaattacc cgcgtgcagg atcacattcc gcatttcgtt cgcgagctgt ttgcgaaaga
     8221 catgccgatt cgtattattt ctgattttgc catggcgcat tcggttactt cctttgcgct
     8281 caagtggggc gacggcatcg aagctgagca tgcaaaagca aaagaaaaga acatcccctt
     8341 tatcacgcac tgctcagagg gttacgatga agaaacgctg cgcagcgtag aaacactggc
     8401 cgctaagaaa gctctcagca agcagacggt attgattcac ggtatcgcgt tcaccgacaa
     8461 agacatggaa ctgctcgccg aacaccagtg caacgtcgtc tggtgcccgg tctcgaatct
     8521 ttacatgttt gagcagaccg cgcgcgttaa agaactcatg gatatgggta tcaacgtgac
     8581 gctgggtacc gattcaccca tgtcgggaag tttcagtatc tttgaagagt tccgtacggc
     8641 gagggagttc taccaatacg cttacggtcg cgcgattgat gatcgtgcgc tggttgatat
     8701 gatgaccgtg aacgcggcca gggccatgtg cctgcctgac gcgggcgaga tagcagaggg
     8761 caaagctgcc gacctgctgc tgatcgacgg caactcgacg aacccctggg cggcgctgac
     8821 gcatgcagat tacgccgata tcatgctcgt gatcgccgaa ggccagccgc gttacggcga
     8881 catcgccttc gcaccggtct ttgatgcaat gaaaattccc taccagacga tcctggtgaa
     8941 aggggcgcgc aagatcatcg ccggcgatat actcgggctg caaaatcgtg tacggcgcgc
     9001 ggtcaaattc aggaaagaac tcgacttcct gccgatagag gctgcctgat gccattaacg
     9061 cgtaactatt ctgccggttc catcgtttac ttcgccggtg acgtcggcga agatatctac
     9121 gtcatgcaga agggacgtat ctctcttatc tccacttcgc tcgaccaaaa agacgacatt
     9181 aaagaagatg tgcgcaaggg cgaatttttc ggggtaaagt cggctctggg ccgctatccc
     9241 cgtgaagaga cagcgcaggt tttagccgat tcgcaggtgc tggttttcaa actggcggag
     9301 tttgagcagt tcgcgcagaa aaatacccgc attgtactgc agatgctcaa ggtcttttcg
     9361 tcgcagctgc gccgcgtgca caaggcagtg cgcgagttcc tgggggaaca cggtgcttcc
     9421 gaagcttcga tggagctcgt gcgcgtcggt gaatactact acaaagcagg caaaaccgac
     9481 tatgctcttt atgtctttaa tgcgtacctg aaaaattacc cggcaggttc actgaccgaa
     9541 cgtgtccggc gcctggcgca gctggtaaaa gaaggagctc ccttcccgat cgacctgccc
     9601 gatatcaatg aggaagtgca cagcaacgac gttgcccagc gcaccggcca gatcgcgcag
     9661 caaatgcatg acgaccccgc cgacgactat gctatccatg accacggcga tatcactccc
     9721 gatttgcccg acatgggcga tatgcctggc gatttacccg atcttccgcc gccggcaggt
     9781 gatttcgaca tgccgatgcc cgatgatttc g
//
LOCUS       DAOQJF010000090         9807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3655_length_9807_cov_5.447703, whole genome shotgun sequence.
ACCESSION   DAOQJF010000090 DAOQJF010000000
VERSION     DAOQJF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3655_length_9807_cov_5.447703"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLF85_06490"
     CDS             <1..345
                     /locus_tag="PLF85_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE19347.1"
                     /translation="ERMQRLGIAHRAKAYPNSVSGGEAQRTGLARALLMDPPVLLLDE
                     PTGNLDSATEVAIVSVLEDLRNEGLTLICISHSEYIMKRADRVLRLSNGQFSDSMKAA
                     PKKRRKTGGAEA"
     gene            342..1709
                     /locus_tag="PLF85_06495"
     CDS             342..1709
                     /locus_tag="PLF85_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19348.1"
                     /translation="MNLVRFLLRRLYLVVFIARKHFLRNALSSVGLFLSLLVVITILG
                     VLDPIKNMIRSKMESSLPPQTLRLVPDLSVVGKGNATWGKFKKEQDNLMHINSRMIER
                     AKNWGQPGDVRSVTATQLLQQPAIGRFEDPILSQLGFSFDLVLQGVPQKMFRPLMRCK
                     APYGGSKTEDKGKEIDEIPVLVPESYLEIMYAYAMINGLPNISAQSLVGLRLRALLGQ
                     SIVGTKWSVTEDAFLVVCGFAPPGVVSALGVPLEWVQKKHRERKQMNALNSFDQLFVE
                     IASEKVLPGILKAAKTERLRIPEQSKKFDSILKSLEKLDLIFMVVAVVLGALAAIALA
                     NSFALLAVQNRYEFGLYLVFGSSMLFLWFLLAIEGALWGFIHSSLAIALSEPALRLLQ
                     QHLAELPWLTKLSTADLSELKLELGTAQRAMIYTAAIGISALASLIPGAIVLGRRTLS
                     LVKKD"
     gene            complement(1706..2212)
                     /locus_tag="PLF85_06500"
     CDS             complement(1706..2212)
                     /locus_tag="PLF85_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19349.1"
                     /translation="MQKATLEHRFPFAFEKLVDLREERYQNLDIWDMFSAAILLKEED
                     DGNIRRREYKLLIRTALPLFAQKLLASGEALACYEITTLDRQKQQYTSDTRLRIVNSA
                     INFVEQSLYSADGVDRSKRTIDIEASVKIPGIGKLIEKLIVHEFHEQSGVDCERILKF
                     AKERYGLG"
     gene            2261..2764
                     /locus_tag="PLF85_06505"
     CDS             2261..2764
                     /locus_tag="PLF85_06505"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HNE19350.1"
                     /translation="MRFTLRQEFPYPIKTVVAARELRYDDIENQPGLKSQELLGVEHD
                     GPVVITRRLFRFGSAIPDVIKKLVPAAMLEMVDTNYFNTETYLSKFTMHSEYAPDKVK
                     ITATCPYFALAENLTVREYEVHVHVSVPIVGNTVAKAILQSHKEALIKDHEIMLKACE
                     RLSQKAN"
     gene            complement(2790..3941)
                     /locus_tag="PLF85_06510"
     CDS             complement(2790..3941)
                     /locus_tag="PLF85_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19351.1"
                     /translation="MIRTFVQRSLFAIVAIALFSSGCKSTSDKPQENQGWVEVTGIAA
                     IKSGNLGLAKDEALKDAKRVAVQEVLGSMVSARSDVKNFELVRQTVTSKSEGMIETFS
                     IVSSQAVSEVEYHVRIKAKVSEAMINQTIEEVVASQGKPRMMVLVDESFNNKADENKV
                     AQTEIEAQFIAQGFPFVDRATVEKIVSKNRRKIAAALGGNNAAARELGVDAGAEIILV
                     GSSRVKEAGKIAGSQLISVQADLNVRVIDINTGKILTAGQEHGAYPHINAESGGVMAV
                     KKAVTPLREKLVADIVKLWDINRANTIALLVTGLDYEKLMVLRGELTEKVRGVKAVNP
                     KGSAGKAAKLEVEFEGSSFDLADRLLTSGLSIKLKIGDVKPNSLDVSAK"
     gene            4189..4626
                     /locus_tag="PLF85_06515"
     CDS             4189..4626
                     /locus_tag="PLF85_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19352.1"
                     /translation="MRRGTSFSLISRFCAFALAERNQVRGRRALSELHEIDRNDYAAG
                     DHHRHLVCLYGEDARLLRLAAIQLGISVAALFRIALKLYLRRLDMDIHSQRYVSDACL
                     FWRGIKRWIAIPLTATNNLTLPTQRSFTFQSFPPEQRWGYPAG"
     gene            complement(4627..5946)
                     /locus_tag="PLF85_06520"
     CDS             complement(4627..5946)
                     /locus_tag="PLF85_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNE19353.1"
                     /translation="MSKNKKDKISYLCTECGESFAKWSGQCPSCKTWNSLTENRFAVD
                     EGEARPAAAIITESAGDRSIISTGLRQFDQILGGGLMPGAVVLLGGEPGIGKSTLLLE
                     IMRAAPAVYVTGEESLQQVKARAERLGLKGDLDIIQGNSLLQVLKYVEQKKAGLVLLD
                     SIQTAYTDDRSGFAGSPSQIREVTQRIIEFAKARQVAFVLAGHITKDGQIAGPKLLEH
                     AVDTVIYFEQNQQTRYRFLRAVKNRFGATGDIAIFEMTATGFREVESADSLIPVQEIG
                     GIGSCLFPQLEGTRVVPLEIQVLVTPTGFANGRRIGENVELSRIHLIAAILEKFCGLK
                     LSQCDIFVRVNGGTQLSEGAGDLALLFAMASSYLEKPLSKRRAVAGQVSLTGEVRSPG
                     GLEERRRALASWKIPRALWGGVREGSVPGLDEVFIPYLDRELKQLEG"
     gene            complement(6052..6372)
                     /locus_tag="PLF85_06525"
     CDS             complement(6052..6372)
                     /locus_tag="PLF85_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNE19354.1"
                     /translation="MAHSDSSQPHSHMKELALTFAALIVLTIVTVKIAKLDFGGVAIN
                     ISIAMFIASIKAGLVALYFMHLKWEEKLIVGFAALSIPFVILMIATMVWDVSQKTAEL
                     IYAK"
     gene            complement(6382..6597)
                     /locus_tag="PLF85_06530"
                     /pseudo
     CDS             complement(6382..6597)
                     /locus_tag="PLF85_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
     gene            complement(6799..7179)
                     /locus_tag="PLF85_06535"
                     /pseudo
     CDS             complement(6799..7179)
                     /locus_tag="PLF85_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
     gene            complement(7179..8846)
                     /gene="ctaD"
                     /locus_tag="PLF85_06540"
     CDS             complement(7179..8846)
                     /gene="ctaD"
                     /locus_tag="PLF85_06540"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HNE19355.1"
                     /translation="MASVATTADHAHHDHHENFLSKKGLMSWLASVDHKRIGLLYLYT
                     GLIAFIVGGAFALLLRTELMFPGKTFVDEKTYNVFFTLHGTIMIFVFIVPGIVASFGN
                     IFMPIMIGARDVAFPVLNRFSYQIYVAGIAVILYGLVNPFGGGPADTGWTFYTPYSTK
                     TNTSVLTLTFGAFLMGFSSILTGLNFVVTIHKMRAPGMTWTRMPLFVWAIYSTSLIQV
                     LATPVIGITLLLLMAEKFFGIGFFDPALGGDPVLFQHFFWFYSHPVVYIMILPAMGVV
                     SEILPVFSRKPIFGYKAIAWSSLAIAGVSFIVWAHHLFVSTLSSSAAIFFSFLTYLVA
                     IPTAIKVFNWITTLYGGQIDLKTPMLYALAFVFLFAIGGLTGLPLAAPAVDVSYHDTY
                     FVVAHFHYTIQGGTIISLMAALYYWMPKISGRMYNERFGQISFWFVFIGFNLTFIPQF
                     LMGTQGMPRRYYDYEPMFATYHMLSTIGAYLLGTGYFTAILNIVISSFKGKPCGDNPW
                     NANTLEWKISSPMPEHNFHEIPTITEWPYEYGRDGEPTKYSAKKGAH"
     gene            complement(8856..>9807)
                     /locus_tag="PLF85_06545"
     CDS             complement(8856..>9807)
                     /locus_tag="PLF85_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNE19356.1"
                     /translation="YFVIKYRRKKGEPNKQTLYLTHNTKAEIIWTVLPTLILGVIFYF
                     GVVSFVKMRNMPKDGEIIQLTARQWLWDIKYPYCVKNEKTKAERCIKLSSKNVNPTED
                     SIKAGKLQDALQKEQTAFVIAKGRKYVMQMTSTDVIHSFAIPSFYVKQDVVPGLTSRI
                     WFEPTQTGEFVITCNEYCGDKHSGMLGKFKVVEPAEFDQWKAEKLAALQKELDQNASG
                     GPVDETALIAKGKELYGAKGCTACHSLNGSPGAAPTFKGLYGKEEVLTTGKIKVDDAY
                     LAESMLKPAAKVVKGYAPMPNMGVEEGDVKPLIAFIKSLK"
BASE COUNT         2304 a         2652 c         2606 g         2245 t
ORIGIN      
        1 gaacgcatgc aacgcctcgg cattgcccac cgtgcgaagg cataccccaa ttctgtctcg
       61 ggcggcgaag cacagcgcac cggtctagcc cgcgccttgc tgatggatcc acccgtgtta
      121 ctactcgatg agccaaccgg caacctggac agcgcaactg aagtggcgat agtttctgtg
      181 ttagaagatt tgcgcaatga aggcctcaca ctaatttgta tttcgcacag cgaatacatc
      241 atgaagcgtg ctgatcgggt cctgcggctc agtaacggcc agttttctga cagtatgaaa
      301 gctgccccca agaagcgcag aaaaaccggc ggagcagaag catgaatctc gtccggtttc
      361 tgctcaggcg tctctacctc gtggtgttta tagcccgcaa acattttttg cggaatgccc
      421 tgtcttccgt agggcttttc ctttcgctcc ttgtggtcat taccattctc ggcgtactcg
      481 atccgatcaa gaacatgatc cgctccaaga tggaatcgag cctgccaccg cagacactcc
      541 gcctggtacc agatctttca gtcgtcggca aaggcaacgc tacatggggc aagttcaaga
      601 aagaacagga caacctcatg cacatcaact cgcgcatgat cgaacgcgcg aaaaactggg
      661 gccaaccggg cgatgtgcgg agtgtcaccg caacgcaact cttacagcaa ccggcaattg
      721 gccgctttga agacccgata ctttcgcagc tgggctttag cttcgacctg gttctgcagg
      781 gtgtgccgca gaagatgttc cggccgctga tgcgctgcaa ggcaccatat ggcggcagca
      841 aaaccgaaga caagggcaaa gaaatcgacg agattcccgt gctcgtacca gaaagttacc
      901 tcgaaatcat gtatgcctac gcgatgatca acggcctgcc caatatctcg gcgcaaagcc
      961 ttgtgggtct gcgcctgcgc gcattgctcg ggcagagtat cgtcggcaca aagtggtcgg
     1021 tgacagaaga tgcgttcctg gtggtgtgcg gctttgcgcc accaggcgtt gtttcagccc
     1081 taggcgtgcc gctcgaatgg gtacagaaga aacaccgcga gcgcaagcag atgaatgcgc
     1141 tcaacagctt tgatcaactc ttcgttgaga ttgccagcga aaaggtcctg ccgggcattc
     1201 tgaaagccgc caagaccgag cgcctgcgca ttcctgagca gagcaagaag tttgattcga
     1261 ttctcaagtc gctcgagaaa ctcgacctga tcttcatggt cgttgctgtt gtcctcggtg
     1321 cgctggcggc aattgcactc gccaactctt ttgcgctgct tgcagtgcaa aaccgctacg
     1381 agtttgggct ctacctggta tttggttcgt cgatgctgtt tctctggttt ttgctggcga
     1441 tagaaggcgc gctatgggga ttcatacact cttcactggc aattgccctg tcagagccgg
     1501 cgctccgcct gctgcaacag cacctggccg aattgccatg gctcaccaaa ctttcgaccg
     1561 cggacctcag cgaactcaaa ctcgaactgg gcactgcaca gcgcgcgatg atctatacag
     1621 ccgcaattgg catttctgcc ctggcttctt tgatacccgg tgcgatcgta ctgggacggc
     1681 gcactctctc gttggttaag aaagactaac cgagcccgta gcgctctttc gcaaacttca
     1741 gtatacgctc gcaatcgacg ccgctctgct cgtgaaattc atgcacaatc agcttttcga
     1801 tcagcttacc aattccggga atctttacgc tcgcttcgat atcaatggtg cgcttgctgc
     1861 ggtcgacgcc atcggctgaa tagagcgact gctcaacgaa gtttatcgct gaattcacga
     1921 tgcgtaaccg tgtatctgac gtgtactgct gcttctgcct gtcgagtgtc gtgatttcat
     1981 agcaggcaag cgcctcgccg ctcgcgagca gcttctgcgc aaacaggggg agtgcggtgc
     2041 gaatcagcag cttgtactcg cgccggcgaa tattgccgtc gtcttcttct ttgaggagta
     2101 tcgcagcaga gaacatatcc cagatatcca gattctggta acgctcttcg cggagatcca
     2161 ccagcttttc aaacgcaaac ggaaaccggt gttccaaggt ggctttctgc acgagtgaga
     2221 atccgaaaag acagagtatc ggtcaaaaac tttatgcact atgcgtttta cgctgagaca
     2281 ggaattccct tacccgatta agaccgtagt tgccgcccgc gagctacgct acgacgatat
     2341 agagaatcaa ccaggactaa aatcgcagga attactgggg gtagaacatg acggcccggt
     2401 ggttatcacc cgccgcctgt ttcgtttcgg tagcgcgata cccgatgtga tcaagaaact
     2461 tgtacctgcg gctatgctcg aaatggtaga taccaactat ttcaatacag aaacctacct
     2521 gagtaagttc acaatgcact ctgaatatgc ccccgacaag gtgaagatca ctgctacctg
     2581 cccctatttt gcgctcgcag agaatctcac agtgcgcgag tatgaagtac acgtgcacgt
     2641 cagtgtgccg atcgttggca acacggtggc gaaggccatt ctgcaatcac ataaagaagc
     2701 cctcatcaaa gatcacgaga tcatgctgaa ggcatgcgag cggctgagcc agaaggccaa
     2761 ttgacgcgcc ggcggcacaa gccggtattt tacttcgcgc tgacgtcgag cgagtttggc
     2821 ttcacgtcgc cgattttcag tttaatcgaa agacccgaag tcagtaaccg atcagcgaga
     2881 tcgaacgaag aaccttcaaa ctccacctcg agcttggcgg ccttacctgc agaccccttg
     2941 gggttcaccg ctttaacacc gcgcactttt tcggtcagtt cgccgcgcag caccatcagc
     3001 ttctcatagt cgagccccgt aacgagcaaa gcgatcgtgt tggcgcggtt gatatcccag
     3061 agttttacga tatcggcgac cagcttctcg cgcagcggcg ttacggcttt cttgacagcc
     3121 atcactccgc cgctctctgc attgatgtgc gggtaggcac cgtgctcctg gccagcggtg
     3181 aggatcttgc cagtgttgat gtcaatcacg cgtacattga ggtcagcctg tacggagatc
     3241 aactgcgaac cggcaatctt gccggcctct ttcacgcggc tcgagcccac cagaatgatt
     3301 tctgcacccg catctacacc caattcgcgc gccgcagcgt tgttgccacc aagcgcggca
     3361 gcgatcttgc ggcggttttt gctgacgatc ttctcaacgg tcgcgcggtc tacgaaagga
     3421 aatccctgtg caatgaactg cgcttcaatt tcggtttgcg caactttgtt ttcgtctgct
     3481 ttgttgttaa agctttcgtc gacgagcacc atcatgcgcg gtttgccctg cgatgctacg
     3541 acttcttcaa tcgtctggtt gatcatcgct tcgctgactt ttgccttgat gcgtacgtgg
     3601 tattcaacct ctgaaacagc ctgcgaagaa acgatagaaa aagtttcgat catgccttct
     3661 gatttgctgg taaccgtctg ccgcacgagc tcgaagtttt tcacatcgct gcgggcagac
     3721 accatactgc cgagtacttc ctgaacggcg acccgcttgg catctttgag cgcttcatct
     3781 tttgccagac ccagattacc gctcttgatg gcggcgatgc ccgtgacttc tacccagccc
     3841 tggttttcct gcggtttgtc agaggtcgat ttgcagccgc tgctaaacag tgcgatggcg
     3901 actatggcaa atagtgagcg ttgaacgaag gtgcggatca tacagcagct cggtcaaacc
     3961 gattattccc gtaaagctaa tctggctttc gcggctctgg aattccataa caggaaaaaa
     4021 ctccgcttca gtgagaaaaa aacggcgttt accggtattt aatatatagg gggatctatg
     4081 aaatgcacgc ggcaaccaga gtttctggca cacaagccta caaaaatcac agaagtttgg
     4141 gaggtcagaa tgcctaggtc gctctggcgg agggtttgtg cgacggcaat gcggcgtggc
     4201 acgagctttt ccctgatttc gcgcttctgc gcattcgccc tggcggaacg caatcaggtg
     4261 cgagggcgca gggcgttgtc agaattgcac gaaatagatc gaaatgatta cgcagcagga
     4321 gatcatcacc gtcacctggt ttgtctttat ggcgaagatg ccaggttatt gcgcctcgcc
     4381 gcaatacaat tgggaatatc cgttgcagcg ctcttccgaa ttgctctgaa actatacctc
     4441 cgtcgtcttg atatggatat ccatagccaa cgctacgttt ccgacgcctg cctgttttgg
     4501 agaggtatca aacgctggat cgccataccc ctaaccgcca caaataatct cacactaccc
     4561 acgcagcgca gcttcacctt ccagagcttt ccaccagaac agcgctgggg atacccagcg
     4621 ggctgactaa ccctccagct gcttcagttc gcggtccaaa tacggaatga atacttcatc
     4681 gaggccaggg acagaaccct cacgcactcc accccacaag gcacgtggaa tcttccacga
     4741 ggcgagggca cgccggcgct cttcgagacc acctggactg cgtacctcac ccgtgagaga
     4801 aacctgcccc gcgactgcac gccgcttgga cagcggcttt tcgaggtaac tcgaggccat
     4861 agcgaagagc agagcaaggt cgccggcgcc ctcgctcagt tgagtgccgc cattgacgcg
     4921 tacgaagata tcgcactgtg aaagtttcag accgcagaat ttttccagta ttgccgcaat
     4981 caggtgaatg cgcgagagct ctacattttc accgatgcgg cgaccattgg caaagcccgt
     5041 gggggtcacc aacacctgga tctcaagcgg caccactcgc gtaccctcga gctgaggaaa
     5101 caggcaactg ccgataccgc cgatctcttg cacgggaatc agcgaatcgg cgctctcaac
     5161 ttcgcggaaa ccggttgccg tcatctcgaa aatcgcaatg tccccggtgg caccaaagcg
     5221 gttcttcact gcacgtaaaa aacggtagcg cgtctgctgg ttctgctcaa agtagattac
     5281 ggtatcgacc gcgtgttcca gcagcttcgg cccggcaatc tgcccatctt tggtaatgtg
     5341 tcccgcaagc acaaatgcca cctgccgcgc cttggcaaac tcgataatgc gctgggtaac
     5401 ctcgcgaatc tgcgagggag atcctgcgaa accactgcgg tcgtccgtgt aggcagtctg
     5461 tatcgaatcg agcaagacga gccctgcctt tttctgctcc acgtacttta gtacctgtag
     5521 caggctgtta ccctggataa tgtccaaatc gcccttgaga cccagccgct ccgcgcgcgc
     5581 cttcacctgc tgcaggctct cttcgccggt gacatagacc gccggagctg cgcgcatgat
     5641 ttcgagcagc agtgtcgatt taccaatacc cggttcacca ccgagcagaa cgacggcacc
     5701 gggcattaag ccgccgccga gaatctggtc aaactgcctc agcccggtcg aaatgatgct
     5761 gcggtcacct gctgattcgg taatgatcgc cgctgccggg cgtgcttcac cctcatccac
     5821 agcaaaccgg ttttctgtca ggctattcca ggtcttgcac gacgggcatt gtccggacca
     5881 ttttgcgaaa gattcaccgc attcagtgca gaggtaggat attttgtctt ttttgttttt
     5941 cgacataaga aataaaaaac ccctccccca acccctcccc tgatcagggg aggggcgaag
     6001 gggtggggtt aatccggaag atttccttcg ggcggcgtat caaccgcgca tttatttagc
     6061 gtagatcagc tccgccgtct tctgtgaaac gtcccaaacc atggtcgcaa tcatcagaat
     6121 cacgaatgga atggaaagcg cagcaaaacc gacgataagc ttttcttccc acttgaggtg
     6181 catgaagtaa agcgccacga ggcctgcttt gatcgacgca atgaacatcg cgatgctgat
     6241 gtttatcgcg acgccgccga agtcgagctt cgcgatctta acagtcacga tggtgagcac
     6301 gatcagagcc gcgaatgtca gcgcgagctc tttcatgtgt gagtgtggtt ggcttgaatc
     6361 tgagtgtgcc attgtttctc cttatgctac caggtaaagc agcgggaaca ggtaaatcca
     6421 gatgaggtcg acaaggtgcc agtaaaggcc gccgacttca acaggagtat accagcccgg
     6481 gtggaattcg ttcttgagac ctttcttcat cacccagatg agcaggccga gaccgataat
     6541 gatgtgcaga ccgtgaatac cggtcatcac gaagtacagg aaaaagaaca tgctcgcgaa
     6601 agcgggcttt gacttatctg tgctgccaat gtttgccagg ttaaaaggca caaccttgca
     6661 cttttcgacc atgatgtctt caccgtggtg tttgtcaaag gtctcttcgc acttgttctc
     6721 tgctttaagc gactggtaga gttctggtgc gatggcttct ttagcgagtt ctacttcgtg
     6781 taccttgtgc gcgtattcaa attttttgtt aataaggaac actacgccgc agacaatcgt
     6841 tataccaacg agaatcagca tcaggcggtt gttgccgcgt ttcaccgctt caatacccca
     6901 ggccacggta aacgagctaa agaggagtat cacggtgttc gcagtaccaa aggccacgct
     6961 gagttctttt gccgaagcat ggaacgccgc tgcgtcacgg tagtaggcaa tcgcgtaagc
     7021 agtaaaaagg atcgcgaaga gcagaatctc cgtcgcgaga aacagccaca taccgaagcg
     7081 cgacatcgag tagaccagcg gggcagggtt gccccacagg ctaccgtcgc gaacagtatg
     7141 accctggtag gttgtgtaac cagcctggtt tgagcccatc agtgtgcccc cttcttggca
     7201 gaatacttgg ttggttcgcc gtcgcggccg tattcgtacg gccactcagt gattgttgga
     7261 atttcgtgga agttatgctc aggcatcggt gatgaaattt tccactcgag tgtgttcgca
     7321 ttccatgggt tgtcgccgca tggcttgccc ttgaagcttg agatcacaat gttcaggatc
     7381 gctgtgaagt agcctgttcc gagcaggtaa gcaccgatcg ttgagagcat gtggtaggtg
     7441 gcaaacattg gctcgtagtc gtagtaacgg cgcggcatgc cttgcgtacc catcaggaac
     7501 tggggaataa aggtcaggtt gaaaccgatg aagacgaacc agaagctgat ctgtccgaag
     7561 cgctcgttat acatacgacc cgagatcttc ggcatccagt agtagagcgc ggccatcaga
     7621 gagatgatgg taccaccctg aatggtgtag tgaaagtgcg ctacaacgaa gtaggtgtcg
     7681 tggtagctca cgtcgaccgc tggcgctgca agcggcagac cagtcagacc accaatcgcg
     7741 aacaggaaca cgaacgccag tgcgtagagc atcggcgtct tgaggtcaat ctggccacca
     7801 tagagggtag tgatccagtt aaagaccttg atcgcagtcg gaatcgccac gaggtaagtc
     7861 aggaatgaga agaatattgc cgcagaagac gacagtgtgc tcacgaaaag gtggtgagcc
     7921 cagacgatga agctcacacc cgcgatagcc agtgaagacc aggcaatcgc cttgtagcca
     7981 aagatcggct tacgtgagaa cacaggtaga atctctgaaa caacacccat cgcagggagg
     8041 atcatgatat atacaaccgg gtgcgagtag aaccagaaga agtgctggaa gagcaccggg
     8101 tcaccgccca atgccgggtc aaagaaaccg ataccaaaga acttctctgc cataagcaga
     8161 agcaaggtga taccgatgac tggggttgcg agcacctgga tcagagaggt tgagtagatc
     8221 gcccaaacaa agaggggcat acgtgtccag gtcatgcccg gggcacgcat cttgtggatc
     8281 gtgaccacga agttcagacc ggtcagaata gaagaaaatc ccattaagaa agcgccgaac
     8341 gtcagtgtga gcacgctggt gttggttttc gtactatagg gagtgtagaa cgtccagcca
     8401 gtgtcagcag ggccgccgcc aaagggattc acgaggccgt agaggataac cgcaataccc
     8461 gcaacgtaaa tctggtagct gaaccggttg agcaccggaa aggccacatc gcgcgcgccg
     8521 atcatgatcg gcatgaagat gttaccaaaa gaggcgacga tacccggaac gatgaacacg
     8581 aagatcatga tcgtgccgtg cagcgtgaag aagacgttgt aggtcttttc gtcgacaaag
     8641 gtcttgcccg ggaacatgag ctccgtgcgc agcaggagcg caaaggcgcc gccgacgatg
     8701 aaggcaatca gaccggtata gaggtaaagg agcccgatgc gtttatggtc aacgctcgcg
     8761 agccatgaca tgaggccctt tttgctcaag aaattttcgt ggtgatcgtg gtgagcgtga
     8821 tcagctgtgg ttgctacaga tgccatttat tccccctatt tcagtgactt gatgaaggcg
     8881 attagcggct tcacgtcacc ctcttcaaca cccatgttcg gcatgggcgc atatccttta
     8941 acaactttgg ctgctggttt cagcatcgat tctgcgaggt atgcgtcgtc gaccttgatc
     9001 ttgccagtgg tcagcacttc ttctttgcca tagaggccct tgaaagtagg cgccgcaccc
     9061 ggcgaaccat tcagcgaatg gcaagcagta cagcccttgg cgccgtagag ctctttacct
     9121 ttcgcgatga gggcagtttc atcgaccggc ccgcccgaag cattctggtc gagttctttc
     9181 tgcagtgctg cgagcttttc agccttccac tggtcaaatt cagcaggctc aactactttg
     9241 aacttgccga gcatgccgga gtgtttgtca ccacagtatt cattacaggt aatgacgaat
     9301 tcaccggtct gtgtaggctc gaaccagatg cgcgaggtca gacccggcac aacgtcttgt
     9361 tttacataga aagagggaat agcgaagctg tggatgacgt cggttgaagt catctgcatc
     9421 acgtacttcc ggcctttcgc gatgacaaaa gctgtctgtt ctttctgcag cgcatcctgc
     9481 agtttgccag ctttgataga gtcttcggta ggattgacgt tctttgacga gagcttgatg
     9541 cagcgctcag ccttggtctt ttcgtttttt acacagtatg ggtacttgat gtcccatagc
     9601 cactggcgcg cagtgagttg gatgatttca ccgtcttttg gcatgttgcg catcttgacg
     9661 aatgaaacaa cgccaaagta gaaaatgaca ccgagaatca gtgtcggcaa cactgtccag
     9721 ataatctctg ccttggtatt atgtgtcagg taaagggtct gcttgttggg ctcacccttt
     9781 ttgcggcggt acttgatgac aaagtat
//
LOCUS       DAOQJF010000091         9769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3677_length_9769_cov_4.534486, whole genome shotgun sequence.
ACCESSION   DAOQJF010000091 DAOQJF010000000
VERSION     DAOQJF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_9769_cov_4.534486"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="PLF85_06550"
     CDS             <1..1980
                     /locus_tag="PLF85_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19357.1"
                     /translation="QNDETAALPPLIDIMNNHRGHETFGPALDLFAEIRRKQGKTAEA
                     LATFEALENEKGLAGKNRKEVLRRHADLLYSEGKFAESGEKYELLAKEFPAGNEGANH
                     YLLLGRTRLKEGKFEAALAALSQLEKNYEDSSAQADALYLKAEISYQQGKFTQALQLY
                     SRFVKKYRNHARATDAEMGIGWTYFELKQFARAADHFRRLLKNEQSSAVRSKALLALG
                     ACLYNLRDLDGAESGYREIITKYPVEKTEYAEAIYQLAWLNFRRNRPQEAGEDFRRYL
                     ALGAEQPRHAEASYFSALCLLQTGNYAEAESALSALTSTPNTPAWIREKSLADLAKSK
                     AALQQLLPARATWQQLLKEYPESAMRDEASYQVTALSLRLGDEQTAISAADKLRIENP
                     ESAWFAETLSEIAAYYRQKRNFSQATAVLKELGAVKKKPDEKLEVQLTRAQVLLDQGE
                     YDEAERMLGAVMRNEDAREETIIRAAQLLFGRFEASGNLAAGSTEAEALAQRFAENGR
                     LRDEMILSTARFRLLQQQYAECRDLLLPLLKNRETAGRARFMLGEISLALGDTTKAID
                     YFRQVSQKQDASIWLKARLMVGEILYSRKDFEEAAREFSRIAYAENRDVIIYEKSLWR
                     AALAFRAIGKQREFETFRAKLREAFPQSPYNKELE"
     gene            1982..2713
                     /gene="surE"
                     /locus_tag="PLF85_06555"
     CDS             1982..2713
                     /gene="surE"
                     /locus_tag="PLF85_06555"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNE19358.1"
                     /translation="MRILISNDDGLSFPGLLRLREKLEQFGETWVFAPAEEKSATSMA
                     LSIHNDVPVIRLDERTFEVDAFPVDCVNVALHGGFAPPFDFCVSGINKGVNMGQDIWY
                     SGTVGAARHCFLHGVPSFAVSSGYLDAAGDFTKVAEIFSEVFARFSSNADQNFLLNVN
                     IPMTAQDAGDIKWAKLGRRIYRDNYQRVTREDGSMLLNLGGSVLSHGGETETDFYWFD
                     KGCTVITPLTIDATDYEKLKLHIPG"
     gene            2670..3479
                     /locus_tag="PLF85_06560"
     CDS             2670..3479
                     /locus_tag="PLF85_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19359.1"
                     /translation="MPQTTKNLSSISPDKQRKTISISEYLNPASSNPLLKKQPSAQSE
                     LSLQQVEELSRIKHEAKRAYRRKDFVQALARFEDATTIVPGDLEFSYYQALCYFQLHQ
                     WDRAEEMFRRIMELDELRLLPDVRKLLALTLLKRKKFSEAEKILTEATRDRKSDLQLM
                     NMLGYCLERQNKLFEAEKVLSRVIENDPENANGLNSLAYVYCRLEKNYTDALSMVKKA
                     LTKEPKNASYLDTLGMLYANRGNTAAARKTLKRALEHAPGNAEILSHLTSV"
     gene            complement(3491..4267)
                     /locus_tag="PLF85_06565"
     CDS             complement(3491..4267)
                     /locus_tag="PLF85_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE19360.1"
                     /translation="MDAVQVDRICLMNGRVLYVGRLPDLADHRQAAGLLCVGLERPFR
                     VLANGKVHVTRSAVVLPQVMHALELDGGLCAFLLVDPDHRDFDLLRAYEARSSEEGIA
                     TDLRDENELMQKIQPVADHSGTDLPAEVWQNLVLGSSSGHGFDHRLRIVIEELLRNSL
                     ESIPVERLAELAGISPSRLAHLFKEHVGIPIRMFRAWARLKTAALLLKEGANLTEAAH
                     GAGFFDSAHFTNTFRENFGLPPSAVFSPNRRLIWHIHPAQ"
     gene            4490..5917
                     /locus_tag="PLF85_06570"
     CDS             4490..5917
                     /locus_tag="PLF85_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE19361.1"
                     /translation="MADKNVKSEIQRIFELQKKFKWSLRQTTAQERREKLKKLKEKIL
                     AESENLYQALWQDYKKPREEADLAEVLPIMAEIHDSIKNLPDWMRPKVVETPIHLGTA
                     RSEIHYEPLGQTLIIAPWNYPFNLSIAPLISAIAAGNTVILKPSEYAPHTAEFLNRIL
                     KDLFYEEEVAVIQGDAAVSTELLKLPFDHMFFTGAPNIGKVVMRAAAEHLSVCTLELG
                     GKSPVVVENTADIREAGRRIAWGKWVNAGQTCIAPDYVLVSEDKKLALIDAIHDTVKG
                     FYGQTPIEWRNSADFCRIVNRRHFDRIKGLIDDAKKHGGQVAFGADTDEHDNYISPTV
                     ITDIGEGAKILEEEIFGPILPIVTYRHLDEALEYIRKGEKPLALYVFSSSESTVEHVL
                     KNTAAGGTSVNDCLMQFVNPKLPFGGCNNSGLGRYHGHHGFKAFSHERAVFRQSRLFN
                     PMQIFYPPYGGQFRETFQKFIRHIM"
     gene            complement(5923..6783)
                     /locus_tag="PLF85_06575"
     CDS             complement(5923..6783)
                     /locus_tag="PLF85_06575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE19362.1"
                     /translation="MTCALCGGTTSPHLDKAGADGRVWHIVRCNSCTAWQIQPLPGNE
                     ELAALYQNEYFKKRSDRGYADYTSEKIRRSVVSTLEKNLNDLGFFSWEQRLSSRRLLE
                     VGCAAGHAVGYFTARGWQALGIDVAREMVEAGRSQGYNLQCADFLTFDFAAAKFDLIT
                     HWATLEHLPDAEKFLRRMASLLAPGGKIYLSTCNTGYFARHYGSAWRYLNLPEHVFYF
                     NKKSLAQLAAKCGLKMTRAFSYGSGFTTREDASWWYRLQKRVADKLARYCLSGDMIVI
                     ELTGDASHPR"
     gene            complement(6780..8174)
                     /gene="rsmG"
                     /locus_tag="PLF85_06580"
     CDS             complement(6780..8174)
                     /gene="rsmG"
                     /locus_tag="PLF85_06580"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNE19363.1"
                     /translation="MRGIPGVNAASVAQLCRHWAMMVESDDRTDLTRLEHERDIALKH
                     YLDCALPLKFTTLPSPLLDIGTGAGFPGLVLKILSPQTHVILGEVRPKRTRFLQAVID
                     ELGLKGVEVFAHRIGPQFPLEINGVVTRALESCRDTLYRVQPFLPQGGRVILLKGPRG
                     EEEVAPAIKELSDFAHEATYRYRLANTQNERRLIIFRRERESIRKATQSAGAAPRETE
                     ITSRANDVFKSLLALGEARGIRKQERYLVSGEKLVEEILSSPALAANLDSIIRDAGHA
                     PVKTNARQLVLAPALFRELDSLGTRSPLALMRLPQLPTWNAESDRSGVTVFLPFQDPS
                     NIGAAIRSAAALGADRVVLLKEAALPFLPKAIRAAANAVFHTRLMLGPSLAELVRSVP
                     GILRLDMAGADIAEVSLDADVLLLPGVEGYGFAETGQTTPVTIPIAGEVESLNAQSAL
                     SIALYEIMRRRSAK"
     gene            8237..9040
                     /locus_tag="PLF85_06585"
     CDS             8237..9040
                     /locus_tag="PLF85_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE19364.1"
                     /translation="MVFDASRILAADESFDLRRISKIVQSGHGCVAALSPLVVHEDNH
                     QLVICKPAGWLSQSDQTKDLSVNEIFAAYIREKYAKPGNVFCAAVQRLDRPAQGLMVL
                     ARTSKGAARISEQIRLGQFEKKYRVLTTKPLLARGQVAKRIVLSADMRKVDRMAQRVA
                     GAGSAARENPAGDRYLLSARLIAAVKETYHYEVEIEGGKFHQIRALFAAHGAPLLGDV
                     KYGGRPLTHKRDRIALVASLLRYQHPTADRQQLFAIDEATLRRLPGYFI"
     gene            9072..>9769
                     /locus_tag="PLF85_06590"
     CDS             9072..>9769
                     /locus_tag="PLF85_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19365.1"
                     /translation="MSKRFAAFTRGFSAAFAFAICASATSAQDKQGTSGAGSIEIIRA
                     SEAEYVTVGEGDEGIFILRGGIIVRSGTSYLRAETVKINTKTGEIFGEGKVSFEAEDN
                     RLTGEKFYYDNKHDAGVVYSANATADPFFLKGSVIKQVESNRYIARDAYFTTCNEKYP
                     HYYFKAKKLWLYKNNELAAMSAIYYVGQTPVFYWPLLLQSDTGTGVITQYGNNPTRGH
                     FLQNTWNFSNPLSQG"
BASE COUNT         2256 a         2871 c         2745 g         1897 t
ORIGIN      
        1 cagaacgacg aaacagcagc tcttcctccc ctcatcgaca tcatgaacaa ccaccggggt
       61 catgagacat ttggcccggc gctcgatttg ttcgctgaga tacgccgcaa acagggcaaa
      121 accgcagagg cgcttgccac ctttgaagcg ctcgagaatg aaaagggcct cgccggcaaa
      181 aacaggaaag aagtcctgcg ccggcacgca gacctgctct acagcgaagg gaaatttgcc
      241 gagtccggcg aaaaatacga gttgttggcg aaggagttcc cggcgggcaa tgaaggagcc
      301 aaccattacc tgctgctcgg gcgcacccgc ctgaaagaag gaaaattcga agctgctctc
      361 gcagctctct cgcagctcga aaagaactac gaagacagca gcgcgcaggc agatgcgctc
      421 tacctcaagg ctgaaatttc ttaccagcaa ggcaagttca cacaggcatt acagctctac
      481 tcccggtttg ttaaaaaata ccgtaaccat gcgcgcgcaa ccgatgccga aatgggtatc
      541 ggctggacgt acttcgagtt gaaacaattt gcgcgcgctg ccgaccattt ccggcgcctg
      601 cttaagaacg aacagagctc cgccgtgcgc agcaaggcat tgctggctct cggcgcctgc
      661 ctctataacc ttcgcgacct cgacggcgcc gaatcaggtt accgcgagat catcacgaag
      721 tacccggtag aaaaaactga atacgcagaa gccatctacc agctggcctg gctgaacttt
      781 cgccgcaaca ggccacagga ggccggggag gattttcgcc gctacctggc actcggcgca
      841 gaacaaccac gccacgccga agcatcgtat ttttccgctc tttgcctgtt gcagaccgga
      901 aattacgcag aagcagaatc tgccctgtcg gcgctgacca gcaccccgaa cacacctgcc
      961 tggatcaggg aaaagtcgct ggccgacctc gcgaaaagta aagccgcgct gcaacagctg
     1021 ttaccggcgc gcgccacctg gcagcagctg ctgaaagaat atcccgaatc ggcaatgcgc
     1081 gacgaagcaa gttaccaggt gaccgcgctt tccctcagac tcggggacga acagactgca
     1141 atttctgccg ctgataaatt gcgtatcgaa aatcccgaat ccgcatggtt tgcggaaacc
     1201 ctgtcagaga tagctgccta ctaccggcag aaacggaatt tttcgcaggc cactgcagtc
     1261 ctcaaggagc tcggtgcggt gaagaagaag cccgatgaaa agcttgaggt gcagctgacg
     1321 cgcgcgcagg tgttgctcga ccagggcgaa tacgacgaag ccgaacgtat gctcggggca
     1381 gtgatgcgca atgaagatgc acgcgaagag acgatcatac gggcggcaca gctgctcttt
     1441 ggccgttttg aagcttcggg caacctggca gcaggcagca ccgaagctga agccctagcg
     1501 cagcgttttg ctgagaatgg ccgtctgcgc gacgaaatga ttctttctac agcgcggttc
     1561 aggctgctgc agcaacagta cgcagagtgc cgtgacctgc tgctgccttt gcttaaaaac
     1621 cgggagaccg caggccgggc gcgcttcatg ctgggcgaaa tttccctcgc gctgggtgac
     1681 acaacaaagg ccatcgacta ttttcggcag gtttcacaaa agcaggatgc atcaatctgg
     1741 ctgaaggcac gcctcatggt gggtgaaata ctctactcgc gcaaggattt tgaagaagct
     1801 gcccgcgaat tcagccgcat tgcgtacgct gagaaccgcg acgtaattat ctacgaaaaa
     1861 tccctgtggc gggccgccct cgccttcagg gcaatcggca aacagcggga gtttgaaact
     1921 tttcgggcca aactccgtga ggcatttcca cagagcccgt acaacaaaga actggagtaa
     1981 tatgcgaata ctgatttcca acgacgacgg actgtctttc cccggccttt tgcggctgcg
     2041 cgagaaactc gagcagtttg gtgagacgtg ggtatttgcc cccgccgaag agaagagcgc
     2101 caccagcatg gcgctatcta ttcacaacga cgtacctgtc attcgcctcg acgagcgcac
     2161 ctttgaagtc gacgcctttc cggtcgactg cgtcaacgtg gcgctgcacg gcggttttgc
     2221 acccccattc gacttctgcg tgagcggcat caacaaggga gtcaacatgg gccaggatat
     2281 ctggtacagc gggaccgtag gtgccgcccg gcattgcttt ttacacggtg tcccctcctt
     2341 cgccgtctcg agcggctacc tcgacgctgc cggtgatttc accaaggtgg ccgagatctt
     2401 cagcgaggtc tttgcgcgtt tttcgtcgaa tgcagaccag aatttcctgc tcaatgtcaa
     2461 tatccccatg accgcgcagg acgctggcga cataaaatgg gctaaattgg gaaggcgaat
     2521 ctaccgagat aattaccaga gggttacgcg ggaagacggc agcatgctgc tcaatctcgg
     2581 cggctcggta ctaagccacg gaggggaaac ggagaccgat ttttactggt tcgacaaggg
     2641 ttgcaccgta atcactccgc tcacaatcga tgccacagac tacgaaaaac ttaagctcca
     2701 tatccccgga taaacagcgc aaaactattt ctatcagcga atacctgaat cccgcctcga
     2761 gcaacccgtt gctcaagaag cagccttcag cacagtctga gctctcgctg cagcaggtcg
     2821 aggagctctc gcgcatcaag cacgaggcga agcgcgccta ccggcgcaag gactttgtcc
     2881 aggcacttgc ccgctttgaa gatgcgacca ccatcgtgcc gggcgacctc gagttcagct
     2941 attaccaggc gctctgctat ttccagctgc accagtggga ccgcgcggaa gagatgttcc
     3001 gccgtatcat ggaactcgac gagctgcgcc tgctacccga cgtgcgcaag ttgctcgcgc
     3061 tcacgctgct caagcgcaag aaattcagcg aagcggagaa gatactcacc gaagcgacgc
     3121 gcgaccgcaa gagcgacctg cagctgatga acatgctcgg ttactgcctc gagcggcaga
     3181 acaaactctt tgaggcggaa aaagttcttt cgcgcgtgat cgaaaacgac ccggaaaacg
     3241 cgaatggcct caactcgctg gcctacgtct attgccgcct ggagaagaat tataccgacg
     3301 cgctgagcat ggtgaagaag gcgctgacca aagaacccaa gaacgcctca tacctcgaca
     3361 cgctgggcat gctctatgct aatcgtggca ataccgcagc tgcgcgcaag acgctcaagc
     3421 gcgcgctcga gcatgcgccg ggtaatgcgg agattctctc gcacctgacc tcggtctgat
     3481 ttgtacccgc ctactgcgcc gggtggatgt gccagatcag ccggcggttc gggctgaaga
     3541 ccgcagaagg tggcaggcca aagttttccc tgaacgtatt cgtgaaatgc gctgaatcga
     3601 agaaaccggc cccgtgcgca gcctcggtca ggtttgcccc ctctttcagt aaaagcgccg
     3661 cggtcttgag ccgtgcccac gcgcggaaca tcctgatagg aatgcccacg tgttctttga
     3721 acaggtgcgc caggcgcgac ggagatatgc cggcgagttc agccagcctc tcaaccggta
     3781 tactttcgag cgagttgcgc agcagctctt caatcacgat gcgcagccgg tggtcaaaac
     3841 catgaccact gctgctgccc agcacaaggt tctgccacac ttctgccggc aggtctgtac
     3901 cgctgtggtc tgcaacgggc tgtattttct gcatcagctc gttctcatca cgcagatcag
     3961 tggcaatacc ttcttcagac gaccgggcct cataggcacg cagcagatca aaatcgcggt
     4021 ggtcggggtc aaccagcaga aacgcgcaca atcccccgtc gagctcgagc gcatgcatca
     4081 cctgcggcag caccaccgca gatcgggtca catggacttt tccatttgcc agcaccctga
     4141 acgggcgctc taaacccacg cagagcagcc ctgctgcctg ccggtggtcg gccagatcgg
     4201 gtagccgacc gacatagagc acgcgtccat tcatcaggca aatcctgtcg acctgcacgg
     4261 cgtccattgc cgaagctcag cgggcgtgac cggggggtaa agcggttttt tcgccctttc
     4321 agcaggtttt tccaagactt ttctgtcatt ttgctgtatg gtcttcgggt agctggcaga
     4381 gggcggagca aaccctgtac tgcgactttg gccgcagaac aggtgaaaat ccgccggcca
     4441 gctgtaggta aaaatcttgc cgccgtcagg tggaaagcaa ggggagttta tggctgacaa
     4501 aaatgtgaaa tccgaaatcc agagaatctt tgaactgcag aagaagttca aatggagtct
     4561 caggcagaca accgcgcagg aacgccgcga gaaactgaag aaactcaaag agaaaatcct
     4621 cgctgaaagc gaaaacctgt accaggcact ctggcaggac tacaaaaaac cccgcgaaga
     4681 agctgacctg gccgaggtgc tgccaatcat ggcagagatt cacgattcga tcaagaacct
     4741 gcccgactgg atgcggccaa aagttgtcga gacccctatc cacctcggta ctgcgcgcag
     4801 cgaaatccac tacgagccgc tgggccagac actgatcatt gctccctgga actacccttt
     4861 caacctgtcg attgcccctc tgatcagcgc gatcgccgcc ggcaacacgg tcatcctgaa
     4921 accttcggag tacgctccgc ataccgcgga gttcctcaac cgcatactga aagacctgtt
     4981 ctacgaagaa gaagtggcag tgatccaggg cgatgcagcg gtctctaccg agctgctcaa
     5041 actgcctttc gaccacatgt tctttaccgg cgcgccaaat atcggcaagg tcgtcatgcg
     5101 cgctgcggca gagcacctgt cggtctgtac gctcgagctc ggtggtaaat cgccggtcgt
     5161 ggtggaaaac accgctgaca tccgtgaggc cggccgccgt atcgcctggg gtaaatgggt
     5221 aaacgcaggc cagacctgca ttgcgcccga ctacgtgctt gtttctgaag acaagaaact
     5281 cgcgctgatc gacgcaattc acgatactgt caaaggtttc tatggccaga caccgattga
     5341 gtggcgcaat tcagcggact tctgccgcat cgtcaaccgc cggcactttg accgcatcaa
     5401 gggcctgatc gacgacgcga agaagcacgg tggccaggtg gcgttcggcg ccgacaccga
     5461 cgaacacgac aactacatct cgcccactgt catcacggat attggcgaag gcgcgaaaat
     5521 tctcgaagaa gaaattttcg ggccgatcct gccgatcgtc acgtaccgcc acctcgacga
     5581 agcgctggag tacatccgta agggcgaaaa gccactggcg ctgtatgtct tctcttcttc
     5641 cgagagcacg gtcgaacacg tactcaagaa cactgctgcc ggcggtactt cagtcaacga
     5701 ctgcctgatg cagttcgtaa acccgaaact cccgtttggc ggctgtaaca acagcggtct
     5761 cggtcgctac cacggccacc acggattcaa ggcattctcg cacgagcgcg cggttttcag
     5821 gcagtcacgc ctgttcaacc cgatgcagat cttctaccca ccctacggcg ggcagttccg
     5881 cgagacattc cagaaattta ttaggcatat aatgtgatga atttaccggg gatgcgaagc
     5941 atccccggta agttcaatta caatcatatc gccagagagg caatagcgcg cgagcttgtc
     6001 ggccacgcgc ttctgcagac ggtaccacca cgaagcatct tcacgcgtgg tgaatcctga
     6061 accatagcta aaagcacgcg tcatcttcag gccgcacttc gccgccagct gcgcgaggga
     6121 ttttttattg aaatagaaga cgtgttccgg caagttgagg taacgccagg cactgccgta
     6181 gtggcgcgca aagtagccgg tattgcaggt gctgaggtag atcttgccac cgggtgccaa
     6241 aaggctcgcc atacggcgca ggaatttttc cgcgtcgggc aaatgttcga gcgttgccca
     6301 gtgcgtgatc aggtcgaatt ttgctgccgc aaaatcgaat gtcaggaaat ctgcgcattg
     6361 caggttatag ccctgtgaac gaccggcttc aaccatctcg cgcgcgacat cgatacccag
     6421 cgcctgccag ccgcgcgcag tgaaataacc taccgcgtgg cccgcggcac aacccacttc
     6481 gagcaggcgg cgtgaagaaa gccgctgctc ccacgaaaag aagcccaggt cgttcaggtt
     6541 tttttccagc gttgaaacta ccgagcgacg aattttctct gacgtgtagt cggcgtaacc
     6601 gcggtcgctc cgttttttga agtattcgtt ctggtagagc gccgcaagct cttcattacc
     6661 gggcaacggt tgtatctgcc atgcagtgca cgaattgcag cgtacgatat gccagaccct
     6721 gccgtcggcg ccggctttgt cgaggtgcgg gcttgttgta ccgccgcaga gcgcgcatgt
     6781 cattttgcgc ttcgccgccg catgatttca tagagcgcaa tcgaaagcgc cgattgcgca
     6841 ttgagcgact caacctcgcc ggcaatgggt atcgttaccg gggttgtctg ccctgtctcg
     6901 gcaaaaccat aaccttccac accgggtagc agcaggacat cagcatccag cgacacctcg
     6961 gcaatatcag caccggccat atcgagacgt aaaatacccg gtacagaacg cacgagctcg
     7021 gccagtgaag gcccgagcat gaggcgcgtg tggaaaacag cattcgctgc cgcgcgtatc
     7081 gccttcggca gaaaaggcaa cgccgcttct ttgagcagca ccacccggtc ggcgcccagt
     7141 gcagcagcgg agcggattgc agcaccgata ttcgaaggat cctggaaagg cagaaagacc
     7201 gtcacgccgc tccggtcgct ctcggcattc caggtgggca gctgtggcag ccgcatcagc
     7261 gccagcgggc tgcgggttcc gaggctatcg agctcgcgga aaagtgcggg agccagtacc
     7321 agctgccggg cgttggtttt tacgggcgcg tgtccggcat cgcggataat agaatccaga
     7381 ttggcggcaa gtgcgggtga gctcaggatc tcttctacca gcttctcgcc actgaccagg
     7441 taacgctcct gcttgcgtat tccgcgtgct tcgccgagcg cgagcagcga cttgaagaca
     7501 tcattagcgc gcgaggtaat ctcggtctcg cgcggggccg caccggcaga ctgcgtagcc
     7561 ttgcgaatac tctcgcgctc gcggcggaaa ataatcaggc ggcgttcatt ctgcgtgttg
     7621 gcgagccggt agcggtacgt ggcttcgtgc gcgaagtcac tgagttcttt tatggccgga
     7681 gcgacctctt cttcgccccg gggacctttc agcagaataa cacggccccc ctgcggcagg
     7741 aagggctgca cgcgatagag ggtatcacgg cagctttcga gcgcacgcgt gacgacaccg
     7801 ttaatctcaa ggggaaactg cggaccaata cggtgcgcaa acacctcaac ccctttcagg
     7861 cccagctcgt cgatgactgc ctgcaggaaa cgggtacgtt tgggcctgac ctcccccaga
     7921 atgacatgcg tctgcggtga cagaatcttg agcacgagcc ccgggaaacc ggcgcccgta
     7981 ccgatatcga gcagaggcga aggcagcgtg gtaaatttga gcggcagcgc acagtcgagg
     8041 tagtgcttca gagcaatgtc gcgctcgtgt tccagccggg tcagatcggt acggtcatcc
     8101 gattcgacca tcatcgccca gtggcggcaa agctgcgcga ccgaggccgc attgacgccc
     8161 gggatgccgc gcaacagggc ttcaagctcg gcaggcggta aaactgttgg tttcctgtct
     8221 ctcttctgga ccggggatgg tgttcgacgc gtcacggata ctcgcggcag atgagagctt
     8281 tgatctcaga cgcatatcaa aaatcgtgca gtcgggtcat ggctgtgtcg cggcgctctc
     8341 acctctcgtg gtgcatgagg acaaccacca gctcgtgatc tgtaaacctg cgggctggct
     8401 ctcgcagagt gaccagacca aagacctctc ggtcaatgag atctttgccg cgtacatccg
     8461 cgagaagtat gccaaacccg gcaatgtctt ctgcgccgct gtgcaacggc ttgaccggcc
     8521 cgcgcaggga ctcatggtcc tggcgcgcac gtcgaagggt gcggcgcgca tcagcgaaca
     8581 gattcggctg ggccagttcg agaagaaata ccgggtactg accaccaaac ccctgctcgc
     8641 ccgtggccag gttgcgaaac gtatagttct gtcggccgat atgcgcaagg tcgaccgtat
     8701 ggcgcaacgg gtggcaggtg caggcagcgc tgcccgggaa aatccggcgg gggatcgata
     8761 cctgctttcc gcgcggctga tcgcggcagt gaaagaaaca taccactacg aggtagaaat
     8821 cgaaggcggt aagttccacc agatccgcgc gctgtttgct gcgcacgggg caccgctgct
     8881 gggcgatgtc aagtacggtg gcaggccgct cacccacaaa cgggaccgta tcgcgctggt
     8941 ggccagcctt ctacgctacc agcatcccac cgccgaccgc cagcagctct ttgccatcga
     9001 cgaagcaacc ctaaggagat tgcccggata ctttatttga cggcgtgtcg gcaaacccaa
     9061 tgctaaaatg agtgtcgaag cggttcgcgg cctttacgcg cggtttttct gccgcttttg
     9121 cctttgccat ttgtgcatct gccacgtctg cacaggataa acaaggcact tcgggcgcag
     9181 gttccatcga aattatccgg gcatcagaag ccgagtacgt cacggtaggc gaaggcgacg
     9241 aaggcatttt cattctgcgc ggcggtatta tcgtccggtc gggtacatct tacctgcgcg
     9301 ccgagactgt gaagatcaat accaagaccg gcgaaatatt cggcgagggc aaggtctcgt
     9361 tcgaagccga ggacaaccgc ctcacgggcg aaaagtttta ctacgacaac aagcatgatg
     9421 cgggggttgt ctattccgct aatgctacgg ccgacccgtt ctttttgaaa ggcagcgtga
     9481 tcaaacaggt ggagagcaac cgttacattg cccgcgacgc ctacttcaca acctgcaacg
     9541 aaaaataccc gcactactat ttcaaagcaa aaaaactctg gctctacaag aacaacgaac
     9601 tcgcggcgat gtccgccatc tactacgtcg gacagactcc ggtcttctac tggccgctgc
     9661 tgctgcagtc ggatacaggt acaggcgtga tcacccagta cggtaacaat ccaacgcgtg
     9721 ggcactttct gcaaaacacc tggaattttt ctaacccgct ttcgcaggg
//
LOCUS       DAOQJF010000092         9762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3682_length_9762_cov_4.777789, whole genome shotgun sequence.
ACCESSION   DAOQJF010000092 DAOQJF010000000
VERSION     DAOQJF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3682_length_9762_cov_4.777789"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..665
                     /locus_tag="PLF85_06595"
     CDS             75..665
                     /locus_tag="PLF85_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE19366.1"
                     /translation="MKPEESKPAWKNYPWKDSRERILVGATVAFSRKGFYGTSVREIS
                     YEAGLEQPSIYHHFGNKEKLYWASLRATHLYMIRNLRRQIRDKDTLLGEIRDMFRAVS
                     WFHKEYPEFFSLLFSLVYSSPPTIADQYTSLYGGDIFSFVDQAFRRNPPKTGRLEKYS
                     LTVHTLYAYILSFSGTRPANLHISYYQALRRVFKAL"
     gene            complement(705..2870)
                     /gene="topA"
                     /locus_tag="PLF85_06600"
     CDS             complement(705..2870)
                     /gene="topA"
                     /locus_tag="PLF85_06600"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNE19367.1"
                     /translation="MAEKAKAKAKPKKTALVIVESPAKTKSIGKFLGGKYRVESSKGH
                     LIDLPRSSMGIDIQNNFEPLYITIRGKGKTLGELRKAAKNSSEVLLATDPDREGEAIS
                     WHLGRALSEVNPKISRIEFNEITKEAVLKAVEHPREIDMQRVDSQQARRVLDRLVGYE
                     ISPVLQKKFGSRRFSAGRVQSAALKILCDREAEIEKFVAEEFWEFDGFFSGTDSKARD
                     KETAFRLSQVAGKKTSIGSKDEAEALEKEILAAKYSLVSRKTSERRIKPLAPYITSRL
                     QQDASTRLGFRAQKTMSIAQGLYEGVELGKEGSVGLITYMRTDSTRISATGLEQARGY
                     IAANFEPAYLPEVANFYGKKKDENTQDAHEAIRPTDVTRTPESLEKFLDRDQYRLYAL
                     IWRRFVASQMAPGVDELVTLEVQDSENKFNFRYSSSRQTFTGFRAIYPLGDEKAQYVP
                     NFNEGDSLKLLEFQKQQKFTQPPPRYTEASLIKAMEESGIGRPATYVPTIGTLDKRAY
                     IERKGRQLIPTKLGRVVNDIMAAHFPDIVDLKFTANMEEKLDSIAQGQNNWRQMLGDF
                     YPSFHGTVERAGSEIADQTHLVRIPLGRECPKCGHELMQKLGKNGYFIGCSNFQGGCR
                     FSESIPLGTCPLCGGSVVKKKGKKGRGFFGCANYSTTGCEFTMLETPAKRTCPKCNSI
                     MGQKVRKTGITLTCQNPECKHTIEETAEETPGENPGAES"
     gene            complement(2874..3827)
                     /locus_tag="PLF85_06605"
     CDS             complement(2874..3827)
                     /locus_tag="PLF85_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNE19368.1"
                     /translation="MAVASKKKTNKRTRLAEIGPEVEIRSEEPIVDKSLSKNPYTESS
                     IQWYLEEINKIPLLTREEEDRLARAAIAGDPKAKDRLITSNLRFVVQVAKKYQKFGIS
                     LLDLINEGNLGLIKAAERFDPDMGYHFISYAVWWIRQAIILAINQKAAMIRLPMNRTI
                     DVARIDAIQKDLENELGEEPTLSQIADRLDMPEAEVRWLKQISSDYVSLDVPANADSE
                     NTQISQVRDDKYISPEDEVMQGALKQSLKEILGELTPSEREIIEHRYGLNGKKTLSLT
                     QAGKKFNLSKERVRQIEKLTLLKLQKYGRARHLEDFLNKED"
     gene            3960..5417
                     /locus_tag="PLF85_06610"
     CDS             3960..5417
                     /locus_tag="PLF85_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19369.1"
                     /translation="MKWFLPAIPFLLLLLPNCSGQNSLSDRTRDITINDSGIGISGGA
                     PTFLGDGPVYQNTSVPQDFSLPMPVLTGATDNTIDLGRCYMDYAPAATNLDAQTRRLC
                     GLQFTQIQLELRALFLDLALPDIRAYCRNKLADCQLSGQAFTITVSEPVMRRVQQLFE
                     FYKYTGLEAYFQYNGYEIRNGAKIPFTIDEYNESCQSPYDYRAVIRTRVIGTYGGQAE
                     VRDNAGDVVIYWDKPRHHIAMDFRTVVDLFGFRINLRRTYEYADRLSGVKYTSSVNYW
                     QQGSTVVNANAVVAEPCSNANADCVKFRHLAGEFLGNDTDLGGLEAHEAFYDAQRFSE
                     GIADARGGIIDSYASYAGSVNKERLVFTGKNQTDFLFQSVAGQPLALVLGDSAAIPGN
                     TYLASYGQQPRLGLTLAANLYAAAPFASRSLAWLATGDAREITSVRGLAVAGSSGSWS
                     LRWGNGTYQQGDFAFDGAQVYRTGVQPADALHPLY"
     gene            5485..7371
                     /locus_tag="PLF85_06615"
     CDS             5485..7371
                     /locus_tag="PLF85_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE19370.1"
                     /translation="MQNLPEVFVRSAAKYPDQGFYSKDSHKFFMPTKFPALYERAQAL
                     AIHLIKAGVKPGDRVAIFGDNSAEWLITDMAIQMAGAIVVPRGSDSTPQELEFILNHA
                     EAQICFVEHLRLFKKIGKVLKERKAQILIMDPLFPDKVAAEEGLVVLPRLLQQLPGVS
                     AADAKVLDDLRSKILPSDLFTIIYTSGTTGEPKGVMLTHANMLYQLEVAPKILQMSPR
                     DRILSILPVWHIYERFMLYSAIFAGCHYYYSNKKDLMEDFVRAKPTLMASAPRLWEQI
                     YQKLRERIDKTEAFNRELFDLSYGIKQRLHRAQNVLNGQSAEGEEKGLLSAIAEKTLS
                     LFSLAGLKWPDVYMDSIFLVRVRAMLGGELRGTISGGGALPIHIDEFFNAIGIPVYEG
                     YGMTETSPLIAMRHPGKVIMGTVGFVPDKTTVEIRNEDTGTLVPHGERGVIFVKGPGV
                     MQGYYKNGNATLKVLKDGWMNTGDIGFFTASGALAIRGRAKDTIVLRSGENLEPVPIE
                     TLLAQHPLIEQAVVVGQDQKNLGVLIWPDYDRLIDAGYQVSEFDLKNDLNTYPELITI
                     FKEICHELINEQNGFKSYERISHVRFLPDKLRVGDELTALMKIKRNIVHNKHAALIET
                     MFQE"
     gene            7393..8370
                     /locus_tag="PLF85_06620"
     CDS             7393..8370
                     /locus_tag="PLF85_06620"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE19371.1"
                     /translation="MQIRTSHVKLQTPDHWNIAVYSYIPEKVTRRYPVMLLHGIASNH
                     NVWDFGVPELSYARHLAGQGYAVYAMNLRGRTGSDGPHTGRGKQWSIDDFLLCDLPTV
                     VEYVIEQHNTEKMHWVGHSMGGILGFFYQIRHKASNLQSLTTFATALNYTYSTINHFR
                     SLLDYISALQYFPLKTFYKPMIPFASLNTFWNRFLWNPENMDPEIAHKVLENMIEPIA
                     VNEWNQIKLISAAEGMPRLSGGFPHRADDRRITVPTLMLAGDRDWLCALDGVEWTMDQ
                     LSCERKLIVFGKEYGSSTSYGHMDLVCGKTAPKEVWPKATDWINKRDVL"
     gene            8418..9524
                     /locus_tag="PLF85_06625"
     CDS             8418..9524
                     /locus_tag="PLF85_06625"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNE19372.1"
                     /translation="MPTSFFDRDLEDFFTQITRHNRERVLEPPPAGALDLCSNDYLCL
                     SQHPGVIAALHEGIDLYGAGSTASRLVRGHREVFAGLENKAAEWLGGEAALYFANGYA
                     ANVGVLSAVCDGSYAAFIDRLAHASLADGVRLSGAEKVYFRHNDMAHLREQLGKSKAK
                     KRIIISESVFSMDGDEAPLGELVRLAQEFDALLYIDDAHATGVFGPAGRGLAPVSADF
                     RVATFGKALGLEGAVVVTSARVRRYLMHSARTFVFSTAPLPAVAHAGLTAIEFVRQME
                     QQRAQLQASARMLREGIAHAGYHAGNSTTQIVPLICGSEQHALTLAAQLLHQGFHAKA
                     IRPPTVKESRIRFSLNAGIGSEDIARLLAALGKV"
     gene            9527..>9762
                     /locus_tag="PLF85_06630"
     CDS             9527..>9762
                     /locus_tag="PLF85_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNE19373.1"
                     /translation="MSLIIAGTGTNVGKTMVSAAIMARYASERRVNYLKPVQTGEESD
                     TQTVLQLTGVPPERAYPEFARFLLPASPHFAAESA"
BASE COUNT         2045 a         2704 c         2684 g         2329 t
ORIGIN      
        1 aatcaatagt acaatcaaaa atttcatttt tcgtttgcgc gcccggtcag actgtgggcc
       61 cgtccgttgg cgacatgaaa cccgaggaaa gcaaaccagc atggaagaat tacccatgga
      121 aagattcccg ggagcgtatc ctggttggtg caacagttgc attctcccgc aaggggtttt
      181 acggaacctc ggttcgcgaa atcagctatg aagccggact ggaacaaccc agtatctacc
      241 accatttcgg taacaaggaa aaactctact gggcatccct cagggcaacg cacctgtaca
      301 tgatcaggaa cctgcgcagg cagatccgtg acaaagacac gctgctgggc gaaatccgcg
      361 acatgtttcg cgcggtctcg tggtttcaca aggagtaccc tgaatttttt tcgctgctct
      421 tttctctggt ctactcttca ccgccgacta tagccgacca gtacacaagt ttatacggag
      481 gagatatctt ttcgttcgtc gaccaggctt ttcgccgtaa cccacccaag actggccggc
      541 tagagaaata ctcgctgacc gttcataccc tttacgctta tattctttct ttctcgggta
      601 cccgcccggc gaacctgcac atctcgtact accaggcgct gcgccgggta ttcaaggccc
      661 tttaatcaaa cattgaccgg agcgtcgaag gtagcgtgaa tggttcagct ttcggcgccc
      721 gggttttcac ccggcgtttc ttctgcagtc tcttcaatcg tgtgcttgca ttcaggattc
      781 tggcaggtga gcgtgatacc ggtcttgcgg accttctggc ccatgattga attacatttt
      841 ggacaggtcc gcttggccgg tgtttccagc atggtgaatt cgcagcctgt agtggagtag
      901 ttggcgcagc caaagaaacc gcgtcccttc ttgcctttct tttttacgac gctaccgccg
      961 cagagcgggc aggtaccgag gggaatactt tcggaaaacc ggcagccacc ctggaaattg
     1021 ctgcagccga taaaatagcc attcttgccg agcttctgca tgagctcgtg cccgcacttg
     1081 ggacattcgc gccccagggg aatgcggacc aggtgtgtct ggtcggcaat ttcgctgcca
     1141 gcgcgctcta ctgtgccatg aaatgaaggg taaaaatctc ccagcatctg ccgccagttg
     1201 ttctgtcctt gcgcaatact gtcgagcttc tcttccatgt tcgctgtgaa cttcaggtcg
     1261 acgatgtcgg gaaaatgtgc agccatgatg tcatttacga cgcggcccag tttcgtcggg
     1321 atcaactgcc tgcccttacg ctcaatgtac gcgcgcttgt cgagcgtacc aatcgtgggc
     1381 acgtacgtcg ccggccggcc aataccagat tcttccatcg ctttaatgag cgaagcttct
     1441 gtgtaacgcg gtggcggctg ggtaaacttc tgctgcttct ggaactccag cagttttaat
     1501 gaatcacctt cgttgaaatt cggtacgtat tgcgccttct catcgccaag cgggtagatt
     1561 gccctaaagc cggtaaatgt ctgccttgag ctcgagtaac ggaaattgaa tttgttctca
     1621 gagtcctgta cctcgagcgt tacgagttcg tcgacgccgg gtgccatttg cgacgccaca
     1681 aaacgccgcc agatcagtgc gtagaggcgg tattggtccc tgtcgagaaa cttctcgagg
     1741 ctttcgggag tgcgtgtgac atcggtcggg cgaatcgcct cgtgcgcatc ctgcgtgttc
     1801 tcgtctttct ttttgccgta aaaattggcg acttcgggca ggtaagcagg ttcaaagttt
     1861 gccgcgatat aaccgcgcgc ctgctctaat cctgttgcag agatacgggt cgagtcagta
     1921 cgcatgtagg tgatgagacc gactgaacct tctttgccga gttctacacc ttcgtaaaga
     1981 ccctgtgcaa tcgacatggt cttctgcgcg cgaaagccga gccgcgttga ggcatcctgc
     2041 tgcagtcggc tcgtgatata aggtgcaagg ggtttgatgc gacgctcgga ggtcttgcgc
     2101 gaaacgaggg agtacttagc tgccaggatt tctttttcga gtgcttcggc ctcgtccttg
     2161 ctgccgatgc tggtcttttt accggctacc tggctcaggc ggaacgcggt ttctttatca
     2221 cgtgccttgg agtctgttcc tgagaaaaaa ccgtcgaact cccagaattc ttcggccacg
     2281 aatttttcta tctctgcttc gcggtcgcag agaatcttca gcgcggcaga ttgcacccgc
     2341 ccggcggaaa aacgccgtga gccaaatttt ttctgcaaga ccggtgaaat ttcatagccg
     2401 acgagacgat cgagcacgcg gcgggcctgc tgcgagtcga cgcgctgcat gtcaatttcg
     2461 cgcgggtgtt cgacagcctt cagtactgct tctttggtga tttcgttgaa ttcaatacga
     2521 ctgatcttcg ggtttacttc tgaaagcgcg cggcccaaat gccagctgat cgcttcacct
     2581 tcacggtcag ggtcggtcgc aagcagtact tcggaagaat tttttgctgc ctttctgagc
     2641 tcccccaggg ttttaccttt gccgcggatc gtaatgtaga gtggctcgaa gttgttctgg
     2701 atatctatgc ccatgctcga gcgcggcagg tcaatcaggt ggcccttgga agactccacg
     2761 cggtatttac cacccaggaa tttaccgatg gatttcgttt ttgcgggtga ctcaacaatt
     2821 accagcgccg tcttcttcgg ttttgctttc gctttcgctt tttctgccat gatttagtct
     2881 tctttgttca aaaaatcttc aaggtggcgt gcccggccgt atttctgcag cttgagtagt
     2941 gtgagctttt ctatctggcg gacacgttcc ttggaaagat tgaacttctt gccggcctgg
     3001 gtgagagaga gtgtcttttt accgttgaga ccgtagcggt gctcgataat ttcgcgttca
     3061 ctcggtgtca gttcaccgag tatttctttg agcgactgtt tcagcgcacc ctgcattacc
     3121 tcgtcttcgg gggagatata cttgtcgtcc ctgacctgtg agatctgcgt gttttccgaa
     3181 tcggcgttgg ctggcacatc cagcgaaaca tagtctgagg agatctgttt taaccagcgc
     3241 acctcggctt cgggcatgtc caaccggtca gcgatctgcg acagggtggg ctcttcaccc
     3301 agctcgttct ccaggtcttt ctgtatagcg tcgatgcgcg ccacatcgat cgtccggttc
     3361 atcggcagac gaatcatcgc tgccttctgg ttgatcgcga gtataattgc ctgccgaatc
     3421 caccagactg catagctgat gaagtggtag cccatgtccg ggtcgaagcg ttcagccgct
     3481 ttgatgagcc cgaggttacc ctcgttaatg aggtcaagca ggctaatacc aaacttctgg
     3541 tacttcttcg caacctgaac gacaaagcgc aggttcgatg tgatcaggcg gtctttggct
     3601 ttcgggtcgc ccgcgatcgc cgcgcgcgcg aggcggtctt cttcttcgcg cgtcagcagc
     3661 ggaattttat tgatttcttc gagataccat tgtatactcg actcggtgta tggattcttg
     3721 ctgagcgatt tgtctacaat cggctcttcg ctgcggattt caacttccgg cccgatctcg
     3781 gccaggcggg tccgcttatt tgtcttcttt ttagaggcaa ccgccacaga tgccttagcc
     3841 ggtacttcag cgacggcgta aacaaattgg caattccggt gactgcgggt agggtgtcgg
     3901 gtttccgcgg gcaaacaaat tattggtgga ttttctgtct ttccggcgtc gaataggcca
     3961 tgaaatggtt cctgccggca atacctttcc tgctgctctt gctgcccaat tgttccggac
     4021 agaacagcct ttctgacagg acccgcgaca tcacgatcaa tgacagcggt atcggcatat
     4081 cagggggagc tcccactttt ctgggtgatg gcccggtcta ccagaacacc tctgtaccac
     4141 aagatttttc gctacccatg ccggtactga ccggcgcgac agacaacaca attgacctgg
     4201 gtcgctgcta tatggactac gcgcctgcgg ccacaaacct cgacgcgcag acccggcgcc
     4261 tgtgtggtct gcagtttacg cagatccagc tggagctgcg cgcgcttttt ctcgatcttg
     4321 cgctgccgga tattcgtgcc tactgccgca ataagctggc cgattgccaa ctttcaggcc
     4381 aggcctttac catcacggta tcagagccgg tcatgcggcg cgtgcagcag ctgtttgaat
     4441 tctataaata caccgggctc gaagcttact tccagtacaa cggctatgaa atccgtaacg
     4501 gggcgaaaat tccgttcact atcgacgaat ataatgaatc ctgccagagc ccgtatgact
     4561 accgcgctgt tatcaggacg cgcgtcattg gcacatacgg cggccaggca gaagtgcgcg
     4621 acaatgcggg cgatgtcgtt atctactggg ataaaccccg tcaccatatc gccatggatt
     4681 ttcgcacggt cgtcgatctc tttggttttc gcatcaacct gcgccgcact tacgaatacg
     4741 ctgaccgcct gagcggagtg aagtatactt cttcagtgaa ttactggcag cagggcagca
     4801 cggtggtcaa tgccaacgcc gtcgtggcgg aaccctgctc aaatgccaat gcagactgcg
     4861 taaagtttcg ccacctcgca ggggagtttt tgggtaacga cacagacctc ggaggtcttg
     4921 aagcgcacga agccttttac gatgcacagc gtttcagtga aggtattgca gatgcccgcg
     4981 gcggcatcat cgacagctat gcctcatatg caggctcggt gaataaagaa cgcctggtct
     5041 tcacaggtaa aaaccagacg gattttcttt tccagtctgt ggcagggcag ccgttggctc
     5101 tcgtgctcgg cgatagcgcg gcaataccag gcaatactta ccttgcgagt tacggccagc
     5161 aaccacgtct tggcctgact ctcgcagcga atctttatgc tgccgcacct tttgcatcgc
     5221 gcagtctcgc gtggctcgca accggcgatg cgcgagaaat tacctccgtt cgcggtctgg
     5281 cagtcgcggg ttcatcaggc agctggagcc tcaggtgggg aaatggcacc taccagcagg
     5341 gcgattttgc ttttgacggg gcgcaggtgt acaggacggg agtgcagcca gcggatgcgc
     5401 tccacccgct atattaactg aacaactgtt cattttttca ttctcctcga cagtctgtcg
     5461 gcgtggtagg ccttagccac cacgatgcag aacctgcccg aagtttttgt ccgcagtgcg
     5521 gcaaaatacc ccgaccaggg attttactcc aaggactcgc ataagttttt catgccgaca
     5581 aagttccctg cgctttatga gcgggcgcag gcactggcga tacacctgat caaggctgga
     5641 gtcaaacccg gtgaccgggt ggcgatcttc ggcgacaatt cagccgagtg gctcattacc
     5701 gacatggcaa tccagatggc aggcgccatc gtggtaccgc gcggttctga ttcaacgcca
     5761 caggaactcg aatttatcct gaaccacgcc gaagcgcaga tctgctttgt tgagcacctg
     5821 cgcctcttca agaaaatcgg caaagtgctg aaagagcgta aggcacagat tttgattatg
     5881 gacccgttgt ttcccgacaa ggtggcagcc gaagagggcc tggttgtgct gcccaggctg
     5941 cttcagcaac tacccggcgt gagcgcagca gatgccaaag tcctcgatga cttgcgcagc
     6001 aagattctgc cctccgatct cttcacgatt atctacacct cgggcacgac tggcgaaccc
     6061 aagggagtca tgctgacgca cgcgaacatg ctgtaccagc tcgaggttgc tcccaaaatt
     6121 ctgcagatgt cgccgcgcga ccgcattttg tcgattctgc ccgtctggca catttacgag
     6181 cgcttcatgc tctattcggc gatctttgcc ggctgtcact attactactc gaacaagaaa
     6241 gacctgatgg aagattttgt gcgtgcgaag ccaacgctca tggcatcggc gccgcgcctg
     6301 tgggaacaga tctaccagaa actgcgcgag cgtatcgata agaccgaggc tttcaaccgc
     6361 gagctgttcg acctgtcata tggtatcaag caacgcctgc accgcgcgca gaatgtgctc
     6421 aatggtcaat cggctgaggg cgaagagaag ggcctcttga gcgccatcgc ggaaaagacg
     6481 ctttcattat tttcgcttgc cgggctcaag tggcccgatg tttacatgga ttcgattttc
     6541 ctggtgcgtg tgcgcgcaat gctcggcggt gagctacgcg gcaccatctc aggtggcggt
     6601 gcactgccga tccacatcga cgagttcttc aacgccattg gcattcccgt ctacgaaggt
     6661 tatggcatga ccgaaacatc tccgctcatt gctatgcggc accccggcaa ggtaatcatg
     6721 ggaacggttg ggtttgtgcc tgataaaacc actgtcgaaa ttcgtaacga agatacgggt
     6781 acgttggtac cgcatggcga gcgtggcgtg atcttcgtca agggccctgg cgtgatgcag
     6841 gggtactaca agaatggcaa tgcaacgctg aaggtcctga aagacggctg gatgaatacc
     6901 ggtgatatcg gttttttcac cgctagcggg gcgctggcaa ttcgcggccg ggcaaaggac
     6961 acgatcgtgc tgcggtcagg tgaaaacctt gagccggtgc cgatcgaaac gctgctcgcg
     7021 cagcatccgc tcatcgagca ggcggttgtc gtgggccagg accagaagaa cctcggcgtg
     7081 ctcatatggc ctgactacga ccggctgatc gatgcgggct accaggtgtc ggagtttgat
     7141 cttaaaaacg acctgaacac gtatcccgaa ctgatcacca tcttcaagga aatctgccat
     7201 gaactcatca atgagcagaa cggctttaag agttatgagc gcatctcgca cgtaaggttc
     7261 ttacccgata agctgcgcgt cggcgacgag ctgacggcgc tcatgaagat caagcgcaac
     7321 atcgtgcaca acaagcacgc ggcgctgatt gaaacaatgt tccaggagta gttcatggcc
     7381 acagcagaaa aaatgcaaat tcgtaccagc cacgtaaaac tgcagacccc cgaccactgg
     7441 aatatcgcgg tctatagtta tattcccgaa aaggtaacac ggcgttaccc ggtgatgctg
     7501 ctgcacggca ttgcctcgaa ccacaacgtc tgggattttg gcgtacccga actctcgtac
     7561 gcgcgccacc tggccggaca gggttacgcg gtttatgcca tgaacctgcg cggtcgaacc
     7621 ggcagcgatg gccctcacac agggcgcggc aagcagtggt cgattgatga ctttttgctc
     7681 tgcgacctgc ccacggttgt cgagtacgtg atcgagcagc acaataccga gaagatgcac
     7741 tgggtcggcc acagcatggg tggtatcctt gggttcttct accagattag gcacaaggct
     7801 tcgaacctgc agagtctcac gaccttcgcc accgcgctca actataccta ttcgacgatc
     7861 aaccacttcc gttcgttgct cgattacatc agcgcgttgc agtattttcc gctcaaaact
     7921 ttctacaagc cgatgatccc gtttgcgagc ttgaacacct tctggaaccg tttcctctgg
     7981 aacccggaaa acatggaccc cgaaattgcg cacaaggtac tcgagaacat gatcgagccc
     8041 attgccgtga acgaatggaa ccagatcaag ctgatctccg ctgctgaagg catgccacgc
     8101 ctctcaggcg gattccctca ccgcgccgac gaccgccgca tcaccgtacc gacgctgatg
     8161 ctcgccggcg accgcgactg gctgtgcgcg ctcgatggtg tcgagtggac catggaccag
     8221 ctctcgtgcg agcgtaaact catcgtcttc ggcaaagaat atggcagcag cacgagctat
     8281 ggtcacatgg acctcgtctg tggcaaaact gcgccgaaag aagtctggcc gaaagccacg
     8341 gactggatca acaaacgcga cgtgttatag atatttgcgt ttcagcctga atcacgtcgt
     8401 ggctgcggtg tgatccagtg ccgaccagct ttttcgaccg cgatcttgaa gatttcttca
     8461 cgcagatcac ccgccacaac cgcgagcggg ttctcgagcc gccgcccgcc ggggctttag
     8521 acctctgttc aaacgattac ctctgcctgt cgcagcaccc cggggtcatt gccgcgttgc
     8581 acgaaggcat cgacctgtat ggtgcgggtt caaccgccag ccggttggtc aggggccacc
     8641 gcgaagtttt tgccgggctg gagaataaag ccgccgaatg gttgggcggt gaagctgcgc
     8701 tctattttgc gaacggttat gccgctaatg taggtgttct ttctgcagtg tgcgatggtt
     8761 cctatgcggc gttcatcgat cgcctagcgc atgcctcgct cgccgacggc gttcggctct
     8821 caggagctga gaaggtctat ttccggcaca acgacatggc tcacctgcgc gagcaactcg
     8881 gtaagagcaa agcaaagaag cgcatcatta tttcggagag tgtcttcagc atggacggtg
     8941 acgaagctcc actgggggag ctcgtacggt tggcgcagga atttgacgcc ctgctgtaca
     9001 tcgacgatgc gcatgccacg ggagtctttg gtccggcggg caggggactt gctcccgtga
     9061 gcgctgattt ccgagtcgcg acttttggta aagctcttgg tctcgagggc gcggtggtcg
     9121 ttaccagtgc gcgtgtgcgg cgctacctga tgcacagcgc gcggactttt gtcttctcga
     9181 cggcaccgct gccggcagtg gcgcatgcag gtcttacagc cattgaattc gtgcgacaga
     9241 tggagcagca gcgcgcccaa ctgcaagcca gcgcccgaat gttgcgcgag ggtattgcgc
     9301 acgcgggata ccatgctggc aattcgacga cgcagatcgt gcccttgatc tgtggttcag
     9361 aacagcatgc gctcaccctg gcagcgcaat tgctccacca gggtttccac gcgaaggcga
     9421 tacgcccgcc gacggtgaaa gaatcgcgca tccgtttctc gctcaatgca ggtattggtt
     9481 ctgaagatat tgcccgcctg cttgccgcgc tgggcaaggt ttaaacatgt cgctgattat
     9541 tgcgggtacc gggactaacg ttggcaagac catggtaagc gcggccatta tggcgcgtta
     9601 tgcgagcgag cggcgggtaa attatctgaa gccggtacag actggtgaag aaagtgatac
     9661 ccaaacggtg ctccagctta cgggggtacc gcccgaacgt gcgtaccctg aatttgcccg
     9721 cttccttttg ccggcatcac cgcattttgc tgcggaaagc gc
//
LOCUS       DAOQJF010000093         9680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3719_length_9680_cov_4.994286, whole genome shotgun sequence.
ACCESSION   DAOQJF010000093 DAOQJF010000000
VERSION     DAOQJF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3719_length_9680_cov_4.994286"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PLF85_06635"
     CDS             <1..1418
                     /locus_tag="PLF85_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE19374.1"
                     /translation="KQYPYYLANHPVTTSAASLRVTDKYTGENYAEVALATPEVIDNA
                     IAAAVAAEKPMSQLASFERRAVLEHVLTRVSERREELAHLLCIEAGKPIKDARGEVTR
                     LMDTFRIAMEEATRIGGEVLPLDITARTKGYRGFTKRVAIGACSFISPFNFPLNLAAH
                     KIAPAIAAGCPFILKPASLTPLGALVLGEILAETALPAGAFSILPCSRDGADLFVTDD
                     RLKLLSFTGSAEVGWKMKARAGRKKVTLELGGNAAVIIDADADIDHAVQRTVFGAFYQ
                     SGQSCISVQRILVHESIYEKFRSHLVAATAALKAGNPHDENVFIGPMISETEAKRLKG
                     WIDIAAQGGAHVLCGGGLSGAMLEPTLLENARPGDAICAEEAFGPAAVLEKFSDFSDA
                     LERVNRSRYGLQAGIFTRDIQKAMLAWDTLEVGGVVVGDVPSFRVDNMPYGGVKDSGL
                     GREGIRSAIEDMTEIRLLVVK"
     gene            complement(1424..2581)
                     /locus_tag="PLF85_06640"
     CDS             complement(1424..2581)
                     /locus_tag="PLF85_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE19375.1"
                     /translation="MSRSVALLALAQAIVQSGSIHLTTTGALIGVALAGSEAFSTWPA
                     SLHVLVALAMTMPASFVMARLGRKTGFFIAFVVGLAGVLLAAQGIYARSFWIFAAGAA
                     VMGVLTAFGNYFRFAAVEAVDEKRKPQAISLVLTGGVLAAFVGPYLSTWGANFFPQAV
                     FTGGYLILLPLYALAILIVSGLSFPKPIAVQVSIRRSFKDILHEPKFLGVVFVGASAY
                     LVMVLIMTATPLAMHREHMGLEATTQVIRAHLLGMFVPSFFTGSLIARFGARTIIIAG
                     VLLFAACIGINFHGVSYWHYFVSLVLLGIGWNFLFVSASQMLTTLVRADHMSAAQALN
                     DFIIALGMASAIAITGKLHALVGWQWLNVLTIPLLPIVMIVGLRLTHGSRR"
     gene            complement(2585..3778)
                     /locus_tag="PLF85_06645"
     CDS             complement(2585..3778)
                     /locus_tag="PLF85_06645"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE19376.1"
                     /translation="MQVHGEWDKRFEPLVSAFRQNFADKYEAGAAVCVYQRGAKVVDI
                     WAGLSNRTNSEQWHEDTLVPVFSVTKALASLSFLILARRGKFDYEKPVAHYWPDFAIA
                     GKKDITCRQLLEHRAGLYSLNTPLELSDFRDNYPRVYRALIMQKPLFVPGSGQAYGAQ
                     IWGAYAGELFRHVAHESLGQFFAREVARKLDTDAFIGLPPQHDHQVAKLYPVSTFDRL
                     TALVPDLLLGNTTEGRVARAFIAGDRSIERAYMNPALGSGGLEVFNETWVHRLELPWA
                     NGIANARSLATIMNVLALGGRVGKTQFADAELMRQLTKPNPLRYDLVLRKRLGWNLGF
                     LKEEPELYSPHSEAFGHSGMGGPVTFADPKTRTAFAYVCNKMDYKIRPDKTLRLCKAL
                     YESLG"
     gene            complement(3826..4083)
                     /gene="grxC"
                     /locus_tag="PLF85_06650"
     CDS             complement(3826..4083)
                     /gene="grxC"
                     /locus_tag="PLF85_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNE19377.1"
                     /translation="MPGKVTIYTKDYCPYCDRAKEYFSTRGIAFEEIDVTRDPQVYAE
                     LRERTQHMTVPQIFIDEQFVGGYTDMIDKIRSGELTIAAPN"
     gene            complement(4171..5019)
                     /locus_tag="PLF85_06655"
     CDS             complement(4171..5019)
                     /locus_tag="PLF85_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19378.1"
                     /translation="MRSQEITVGRLKFAYERYEGGKEIFILIHGWCSVRGFWAPVLKD
                     FRQLGTCINLDLAGHYPATTAGDFAGVDLVKVTALQAAAIQQLVGKKKVTVVGHSTGG
                     LIALSLALHYPQLVKRVVAIGPVIHGPVQGPLGFARDLYKLNLHAALHLPFHFIRSVP
                     DAFQNIFETGVHRPKTFFRRADATEYVSAYRDQLLRLPPEAMGAWLTIIDSADLRPAL
                     AGSNVPTLFVTGAHDKVVSPAQAREIARRMPRAAYHEYAQSGHIPILEEEAECFENLR
                     LWLREN"
     gene            5160..5534
                     /locus_tag="PLF85_06660"
     CDS             5160..5534
                     /locus_tag="PLF85_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE19379.1"
                     /translation="MAQRVLVVDDSQLMRQIIAGFLSANGFAIAGEARDGVEALKLAE
                     AVQPDLVTLDIGLPQLGGITVLQKIKEVSPATRVMVVSAIHDKSLVLQALEAGAADFL
                     HKPISEDLLVESARRLTSQPAA"
     gene            5605..6180
                     /locus_tag="PLF85_06665"
     CDS             5605..6180
                     /locus_tag="PLF85_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE19380.1"
                     /translation="MSAEPTDPKAPTQKPLTPVLEAEFQRIYNKSKSRVLNYIQRSVR
                     DRDEALDLLQEVFITFYDKLPELDVSTDRIESWLVKTARNVILTYVRDTHRRLTRDLQ
                     NTQESDTWEKDDQVGKIYQKELEKHIDNFLVSLNDQERNLFILQKIEQVKYNDLEKIL
                     GIPQRTMKRMASNLLQRLKERTTFDRDYLEN"
     gene            complement(6192..6893)
                     /locus_tag="PLF85_06670"
     CDS             complement(6192..6893)
                     /locus_tag="PLF85_06670"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNE19381.1"
                     /translation="MLFEWQCGYYPERLARNHDFSDPHHQFTPEQLTRVLNKGYRRYA
                     TDYYRPYCRNCRECTAYRVIVGEFKPNRSMRRVLKKNSAVEAHWGTPQPTQEKFELYV
                     RYQLSRHKETDVPVTELRRQLAAAMVRQMYTNPPTTLELTTTENGKVVGFAIFDRTLD
                     ALSAVYSAFEPDEPERSFGTLNILLAIEKARVERLPYLNLGLWLEHHPKMAYKRNFQP
                     AEIYRQYRWRPLPEN"
     gene            6971..7972
                     /locus_tag="PLF85_06675"
     CDS             6971..7972
                     /locus_tag="PLF85_06675"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNE19382.1"
                     /translation="MAGKHVVIAGASGAVGIEFMSVLERLNFPVASLRLLATKRSAGK
                     NLRFKGEEITIEEMTKDSFKGADVALFSAGSSVSKEFEKAVTDANCVMVDNSSAFRMR
                     EGTPLVVPEINPEDAKKHSGVIANPNCSTIILLMGVYPLHRKYPVRRLVVSTYQAASG
                     AGWAAMEELKDSTRAVLDGKPFTPQKMPYPYGFNLFSHNTAIDAVGYNEEEMKMVKET
                     KKILSTDKISISPTCIRVPILRAHAESINIEFEGNAPSEDEARKLFTEFPGVRVVDDR
                     AKNHFPMPLEASGEFDCLVGRIRKDVSMPDKALNIFIAGDQLLKGAALNAVQIAALL"
     gene            8003..8422
                     /locus_tag="PLF85_06680"
     CDS             8003..8422
                     /locus_tag="PLF85_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HNE19383.1"
                     /translation="MSNIEIVEKFLAALEAIDLPAAEALLDDNMVYENVGLSVDHGRE
                     AAINTLKGFMRFSDRFEVIMHAIAENGNIVLTERTDILSGPGIHLEFWVCGRFEVRDN
                     KIVLWKDYFDRAAVTGQIAKSMPGFLTTGVWTKLVNR"
     gene            complement(8587..9231)
                     /locus_tag="PLF85_06685"
     CDS             complement(8587..9231)
                     /locus_tag="PLF85_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19384.1"
                     /translation="MKIIEKFLFGLAFLTVSGCATLMGGGASDPVINSDDLAAIQGAN
                     ASNTCLITGLVDGSKEAGSGHSGTIVAGDKTAALVFKSAGALDASVFYALVPAGSFEI
                     KFLSLAEMKKSNESCWGSGNKCIVTYGQPASVTEKNPLPGKHGTLPESYKGTCKGGFV
                     HLGVYEAVVGGLMSSSKFTEMSTSAYMPGERFQSRHFAALREQLKGTAWENLIK"
     gene            complement(9360..>9680)
                     /locus_tag="PLF85_06690"
     CDS             complement(9360..>9680)
                     /locus_tag="PLF85_06690"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNE19385.1"
                     /translation="SGETNRVCDIGLWKYSRHPNYFGEWLVWLGLVVAAIPSWWFLQH
                     KEPMPVWILLGLGLLYIVRIMYWVLNSYSGAEPAEYYSLQKRPGYAEYQEKTNMFFPG
                     KGRM"
BASE COUNT         2267 a         2736 c         2644 g         2033 t
ORIGIN      
        1 tgaaacaata cccatattat ctcgccaacc acccggtgac caccagcgcc gcctcgctgc
       61 gggtgaccga caagtacacg ggtgaaaact atgccgaagt cgcgctcgca acacccgagg
      121 tgatcgataa cgccattgcg gcagccgtcg cagcggaaaa gccgatgtct cagctggcct
      181 cattcgagcg ccgtgcggtg ctcgagcatg tactgacacg ggtcagcgag cgccgcgaag
      241 agctggcgca cctgctctgc atcgaagcgg gtaagccgat caaagacgcg cgcggcgaag
      301 tcacccgcct catggatact tttcgcatcg ctatggaaga ggcgacgcgc atcggcggcg
      361 aagtgctgcc actcgacatc actgcacgca ccaaaggtta ccgcggcttc acgaaacgcg
      421 ttgccatcgg cgcctgttcg ttcatcagcc ctttcaattt tccgctcaac ctggctgcgc
      481 ataagattgc ccccgctatc gcggcgggct gcccgtttat tctgaaaccc gcaagcctta
      541 ccccgctggg agccctggtg ctcggcgaaa ttcttgccga aacggcgcta cccgccggcg
      601 cgttctcgat tctgccctgc tcgcgcgacg gggctgacct gtttgtcacc gatgaccggt
      661 tgaagttact cagcttcacc gggtcagccg aagttggctg gaaaatgaaa gcgcgcgcgg
      721 gtcgtaaaaa agtgacactg gagctcggag gtaatgcagc ggtgattatc gacgctgacg
      781 cggatatcga ccacgcggtg cagcgtaccg tgttcggcgc attctaccag tcgggccaaa
      841 gctgtatcag cgtgcagcgc attcttgtcc acgaatcgat ctacgaaaaa ttccgtagcc
      901 acctcgttgc ggcgacggcc gcgctcaaag cgggaaatcc gcacgatgaa aatgtcttta
      961 tcgggcccat gatttctgaa accgaggcga agcggctcaa gggctggatc gatatagcag
     1021 cgcagggcgg agctcatgtg ctctgcggcg gcggtctctc gggggcgatg cttgaaccaa
     1081 ccctgctgga aaatgcgcga ccaggcgatg ccatctgcgc cgaagaggcc tttggccccg
     1141 cagctgtgct cgaaaaattc tctgatttta gcgatgccct tgaacgcgta aaccgcagcc
     1201 gctacggtct gcaggcgggc attttcaccc gcgatataca aaaggcgatg cttgcatggg
     1261 atactctcga agtaggcggt gttgtcgtgg gcgacgtgcc gagctttcgc gtcgacaaca
     1321 tgccctacgg tggcgtgaaa gattcaggcc tgggccgtga aggtattcgc tcggcaattg
     1381 aagatatgac cgagatacgc ctgctcgtgg tgaagtagca tgcttaacgc ctgctaccgt
     1441 gcgtcaggcg cagaccaaca atcatcacga tcggcagcag aggaatcgtg agcacattga
     1501 gccattgcca gccgaccagt gcatgcagct tgccggtaat tgcgatggcc gatgccatgc
     1561 cgagcgcgat gatgaagtcg ttcaacgcct gtgccgcgct catgtggtcg gcacgcacca
     1621 gcgtcgtgag catctggctg gctgaaacga acaggaaatt ccagccaata cccagcagca
     1681 ccagcgaaac aaaataatgc cagtagctca caccgtgaaa gttgattccg atgcacgcag
     1741 cgaagagcaa taccccggca ataatgatgg tacgggcacc gaagcgggca atgagactgc
     1801 cggtaaaaaa tgacggcaca aacatgccca gcaggtgcgc gcgtatcacc tgcgtcgttg
     1861 cttcaaggcc catgtgttcc ctgtgcatgg cgagaggagt ggctgtcatg atgagcacca
     1921 taaccaggta agcggaagct cccacaaaga caacgcccag gaatttcggt tcgtgcaaga
     1981 tatctttgaa tgaacggcga atagagacct ggaccgcaat cggtttagga aaactcaggc
     2041 ctgatacaat caggatcgcc agagcataca ggggcagcaa gatcaggtaa ccgccggtga
     2101 aaactgcctg cgggaaaaaa ttcgcccccc atgtcgacag gtaggggcca acaaaggctg
     2161 cgagtacgcc accggtaagc accagtgaaa ttgcctgcgg cttgcgcttt tcgtcgaccg
     2221 cctcgactgc agcaaagcga aaataattgc cgaaggcggt cagcacaccc atgactgcag
     2281 cgccggctgc gaaaatccaa aaagaccgtg catagatgcc ttgtgccgca agcaaaacgc
     2341 ccgccaaacc aaccacaaaa gcaatgaaga accccgtctt gcggccaagc cgcgccatca
     2401 cgaacgaagc cggcatcgtc atcgcgagtg caaccagcac gtgcagtgac gccggccagg
     2461 tagagaaagc ctctgaacct gcaagcgcga cgccaatcag tgcaccggtc gtcgtcaggt
     2521 gtatgctacc cgactgcacg attgcctgcg cgagtgcaag cagtgcaacg ctacggctca
     2581 aaggctaccc caggctttca tagagtgcct tgcacaaccg cagcgtctta tctgggcgga
     2641 ttttgtaatc catcttgttg cagacataag cgaacgcagt gcgcgttttg ggatcagcga
     2701 aggtgaccgg cccgcccata cccgaatggc caaacgcctc gctgtggggt gaataaagtt
     2761 cgggttcttc tttcaggaaa ccaagattcc agccaagccg cttacgcaga accaggtcgt
     2821 aacgcaacgg attgggctta gtcagctgcc gcatcagttc agcgtcggca aattgtgttt
     2881 tgccgacacg gcctcccagc gcgagcacat tcattatcgt cgccagcgaa cgcgcattgg
     2941 caattccgtt ggcccacggt aattcgagac ggtgcaccca ggtttcgttg aagacttcga
     3001 gtccaccaga cccgagcgcc gggttcatat acgctctctc gattgaccgg tcgccggcaa
     3061 tgaaagcacg cgcaacccgc ccttcagtcg tattgcccag caagagatca ggcacgagcg
     3121 cggtcaggcg atcaaaggta gacaccgggt agagtttagc aacctggtgg tcgtgctggg
     3181 gtggcagacc gataaacgca tcggtatcta acttacgcgc aacttcccgg gcaaaaaact
     3241 gccccaggct ttcgtgcgca acatgccgaa aaagctcccc tgcgtaggcg ccccagatct
     3301 gtgcgccgta agcctggccg ctgcccggca caaacagcgg cttctgcata atcagtgcac
     3361 ggtagaccct gggataatta tcgcgaaaat cgctgagctc cagtggcgtg ttcagcgagt
     3421 agaggccggc gcggtgttcg agcagttgcc ggcaggtaat atctttctta cccgctatcg
     3481 caaagtcggg ccagtagtgt gcaaccggtt tctcgtaatc aaatttgccg cgcctggcaa
     3541 gaatcaaaaa actcagcgaa gccagcgcct ttgttacgga aaaaaccggc acgagcgtgt
     3601 cttcatgcca ttgttcggag ttcgtgcggt tgctgagacc tgcccagata tcgacaactt
     3661 tcgcaccgcg ctggtaaacg cagactgcgg caccggcctc atacttatcg gcgaagttct
     3721 gtcgaaacgc cgagaccagc ggttcgaaac gtttatccca ttcgccgtgt acctgcatag
     3781 cacctacggg aagaaatgca accgggcgtc gcgctctttt ggtttttagt tgggggctgc
     3841 aatcgtcagt tcgccgctgc ggattttatc tatcatgtca gtgtaaccgc cgacaaactg
     3901 ctcgtcgatg aatatctgtg gtacggtcat atgctgcgtg cgttccctga gctctgcgta
     3961 gacctgcggg tcgcgggtga catcaatctc ttcaaaggcg ataccccgcg tgctgaagta
     4021 ttctttcgcg cggtcacagt aaggacagta gtccttggta tagatggtga cttttcctgg
     4081 catacgcttc tccggaagcc cgttggttaa aatgatacaa aaactcctgc gcagcggcac
     4141 ttttgtatga cgagttcggt tcagcgaccg ctagttttcc cgcagccaga gcctgaggtt
     4201 ctcaaaacac tccgcctctt cttcgagtat cgggatatgc cctgactgcg cgtattcgtg
     4261 gtaggcagcc cgcggcatgc ggcgcgcaat ttcacgcgcc tgtgcaggtg atacgacttt
     4321 gtcgtgcgcc cccgtaacga agagagtggg tacattgctg cccgcgagcg cggggcgcag
     4381 gtcggcgctg tcaatgatcg tcagccaggc ccccatcgcc tcgggtggta gtcgcagcag
     4441 ctgatcacgg taggcgctga cgtactctgt cgcgtcggca cggcgaaaga aggttttcgg
     4501 gcggtgcaca cccgtttcaa aaatgttctg gaacgcatcg ggcaccgaac ggataaagtg
     4561 gaaaggtagg tgcagcgccg cgtgcaggtt tagcttgtag agatcgcggg caaaacccag
     4621 cggcccctgc accggcccat ggatgaccgg cccgattgct accacgcgct tcaccagctg
     4681 cggatagtgc aatgccagcg acagggcgat taatccccct gtcgaatggc cgaccacggt
     4741 aaccttcttc ttaccgacca gctgctgtat tgcagccgcc tgcagtgccg ttaccttgac
     4801 caggtcgacc ccggcaaaat cgccagcggt cgtcgcggga taatgccctg caagatcgag
     4861 gttgatacag gtaccaagct ggcgaaaatc tttcaggacc ggggcccaga agccgcggac
     4921 tgaacaccag ccatggatca ggataaagat ttcttttcca ccctcataac gctcgtaggc
     4981 gaattttagc ctgccaacgg tgatttcctg tgaacgcatg acagtcagcc gccagagcac
     5041 aatgccacgg tcaaatgctt gcttaaaatt gcccggtgca taccctggca ggctcctttt
     5101 tcaggagcct gccagacctt ttgctttgcg gcctgtcaaa atcaccggaa ccgtcgcgca
     5161 tggcacagcg cgtgctcgtg gtagatgact cacaactgat gcggcagatt atcgccggtt
     5221 ttctctcggc caatggtttt gccattgccg gtgaagcgcg cgatggcgta gaagcactga
     5281 aacttgccga ggccgttcag ccagacctcg tcaccctcga tatcgggctg ccacagctcg
     5341 gcggtataac agtgctgcaa aagatcaaag aggttagccc tgccacgcgg gtgatggtgg
     5401 tttctgccat tcacgacaag agcctggtgt tgcaggcgct cgaagcaggt gccgcggatt
     5461 ttctgcacaa gccgatttct gaagatctgc tcgttgaatc ggcccgccgc ctcacatcgc
     5521 agcccgctgc ttagcagccg ctcataaaca gtcgttccct ttacagagtt taccgatgcc
     5581 ttggcgaaaa aataatggct tccgatgagc gctgagccga ccgacccgaa agctcctacc
     5641 cagaagcctc tcacgcctgt tctcgaggct gagttccagc gcatctacaa caagagcaaa
     5701 agccgcgttt taaactacat acagcgttcg gtgcgtgacc gcgacgaggc actcgacctg
     5761 ctgcaggaag tttttatcac cttttacgat aagctgcccg agctcgatgt ctcgaccgac
     5821 cgtatcgagt catggctggt caaaactgcc cgaaacgtga ttctcaccta cgtgcgcgac
     5881 acgcaccgtc gcctgacgcg cgacctgcaa aacacgcagg aaagcgacac ctgggaaaaa
     5941 gacgaccagg tcggtaaaat ctaccagaaa gaactcgaaa agcacatcga taactttttg
     6001 gtgtcgctca acgaccagga acgtaacctc tttatactac agaaaatcga gcaggtcaaa
     6061 tacaacgacc tggaaaaaat tctcggcata ccacagcgca cgatgaagcg catggcctcg
     6121 aacctgttac agcggctcaa agaacgcaca acgtttgata gggattattt ggaaaattga
     6181 cccctgcggg gttaattttc cggtaggggt cgccaacggt actgccggta tatttctgcc
     6241 ggctggaaat tgcgcttgta cgccatcttc gggtggtgct ccaaccagag cccaaggttc
     6301 aggtacggca ggcgctccac ccgcgctttt tcgatcgcca gcaaaatatt cagcgtgccg
     6361 aatgaacgct cgggttcatc gggctcaaag gccgaataga ccgccgacag tgcatcgagt
     6421 gtgcggtcga agatcgcaaa ccccacaacc ttgccgttct cggtcgtcgt cagctcgagt
     6481 gttgtcggcg ggttcgtgta catctgccgc accatcgctg ccgcaagctg ccggcgcagt
     6541 tcagtgaccg gcacgtcagt ctccttgtgg cgtgacagct ggtagcgcac gtacaactca
     6601 aacttttcct gtgtcggctg tggcgtgccc cagtgcgcct caacggcgga gttctttttg
     6661 agcaccctgc gcatgctccg gttgggctta aattcgccga cgatcacccg gtaggccgtg
     6721 cattcgcggc aattccggca gtacgggcgg taataatcgg tcgcgtagcg ccggtaacct
     6781 ttattgagaa cgcgggtcag ctgttcgggc gtgaactggt ggtgcgggtc tgagaaatcg
     6841 tggttgcgcg ccagccgttc ggggtaataa ccacattgcc attcaaagag cataccagcg
     6901 tcaccagggc agcgcccggt acaattgctc tttcgatttt caaggtgtcg cgcgaaaaaa
     6961 agctctccgc atggcaggaa aacacgtggt aatcgccggg gcatccggcg ctgtgggtat
     7021 cgagttcatg agcgtcctgg aaaggctgaa tttcccggtg gcgtcgctgc gcctgctcgc
     7081 cacgaaacgc agcgccggca aaaacctcag attcaagggt gaagagatca ccattgaaga
     7141 aatgaccaaa gattcattca agggtgcaga tgttgcgctc ttctctgccg gcagctcggt
     7201 gagcaaagaa ttcgaaaaag ccgtcaccga cgcaaactgc gtgatggtcg acaatagctc
     7261 cgcctttcgc atgcgcgagg gcactccgct cgttgtgccc gagatcaacc ccgaagacgc
     7321 gaaaaagcac tcgggcgtga tcgcgaaccc caactgctca acgatcatct tgctgatggg
     7381 agtttaccct ctgcaccgca agtaccctgt gcgccggctg gttgtctcga cttaccaggc
     7441 agcttcgggc gcgggttggg ccgcgatgga agaactcaaa gattctaccc gcgcggtgct
     7501 cgacggcaaa ccgttcacgc cacagaagat gccctacccg tacggcttca acctgttttc
     7561 gcacaacacc gcgatcgacg cagtgggtta taacgaagaa gagatgaaga tggtgaaaga
     7621 gaccaaaaaa attctttcga ctgacaagat ctcgatcagc cccacctgta ttcgcgtgcc
     7681 cattttgcgc gcgcatgccg agtcgattaa catcgagttc gaaggcaatg cgccgtcaga
     7741 agacgaagcg cgcaaactct tcactgagtt tcccggagtg cgcgtcgtcg acgaccgcgc
     7801 gaagaaccat tttcccatgc cactcgaagc atcaggcgag ttcgactgcc tcgttggccg
     7861 cattcgcaaa gacgtgagca tgcccgacaa ggcgctcaac atcttcatcg cgggcgacca
     7921 gctgctgaag ggcgctgcac tgaacgcagt acagatcgcg gcgttgttgt agaaaccggc
     7981 aagaaaggca gagacgcagt tcatgagtaa tattgagata gtagaaaagt ttttggcagc
     8041 tctggaagca atcgatctgc ccgctgccga agcgctgctc gacgacaata tggtttatga
     8101 aaacgtcggt ctcagtgtcg accatggccg tgaggctgcg atcaatactc tgaagggttt
     8161 catgcgattc tctgatcgat ttgaagtcat catgcatgcc attgctgaaa acggcaacat
     8221 tgtcctaaca gaacgcaccg atattctttc aggtccgggc attcatctgg aattttgggt
     8281 ctgcggccgc tttgaggtca gagacaataa gatagttttg tggaaagatt attttgatcg
     8341 ggcggcggtt acggggcaaa tcgcaaaaag catgcccggt tttctgacga ctggcgtatg
     8401 gacgaagctg gtaaaccgat agcgtctttt gaggctgcat tccgtagctg cgtttcagcc
     8461 taagctgccg gggcctatgt aatctatctg actcttggga tcttcagcaa agaacaggca
     8521 tggttaaacc agaatcgcaa gcagtacggc gcattgcaaa tccgccgtaa tgcttgctcg
     8581 caagtactat ttgatcaggt tttcccaggc agtccccttc aactgctcac gcagagctgc
     8641 gaagtgacga ctctgaaagc gctcgcccgg catgtatgca ctggtgctca tttcagtaaa
     8701 tttcgaagag ctcatgagac caccgactac tgcctcataa acaccgaggt gtacaaagcc
     8761 acctttgcat gttcctttgt aactttcggg aagtgtgcca tgcttaccag gaagcggatt
     8821 tttctctgtc acggaagctg gttgaccata agtaacgatg catttattac ctgatcccca
     8881 gcagctttca ttgcttttct tcatttctgc aagcgacaga aatttgattt cgaacgagcc
     8941 ggctggtact agcgcgtaaa agacacttgc atcaagcgca ccggcactct taaaaaccag
     9001 cgcggcggtt ttgtcgccag caacgattgt acccgaatgt ccagagcccg cttctttgct
     9061 tccatctacc agcccagtaa tgaggcaggt gtttgacgca tttgcccctt gtatggcggc
     9121 caaatcatcg ctattgatta ccggatcaga tgcgcccccg cccatgagag ttgcacagcc
     9181 actaacggtc aggaatgcta aaccaaaaag aaatttctcg attattttca tgaatcctcc
     9241 atgattgttt ttggcggatt tgtaataagc acatcaaaat gtcaacttta tcttcgcact
     9301 agcaagagtt gctgcttgca cggaaatcgc aactgccatt aggcggtttt acccagacat
     9361 tacatgcgcc ctttgccggg aaaaaacata ttggttttct cctggtattc ggcataaccc
     9421 gggcgctttt gcagcgagta atactcagca ggctctgcgc ccgagtagct attcagtacc
     9481 caatacatga tgcgaacaat atagagcagc cccaagccaa gaagaatcca gactggcatc
     9541 ggctctttgt gttgcagaaa ccaccacgag ggtattgcag cgaccaccag gcccaaccag
     9601 acaagccact caccaaaata attgggatgc cggctgtatt tccagagacc gatgtcgcag
     9661 acacggttcg tttcgccgga
//
LOCUS       DAOQJF010000094         9663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3732_length_9663_cov_5.838156, whole genome shotgun sequence.
ACCESSION   DAOQJF010000094 DAOQJF010000000
VERSION     DAOQJF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3732_length_9663_cov_5.838156"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..973
                     /locus_tag="PLF85_06695"
     CDS             80..973
                     /locus_tag="PLF85_06695"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNE19386.1"
                     /translation="MTQGVAQPGSLAKKKVVWNFDSDTSLLRYNHVHTVCEESRCPNR
                     NECSSLGIATFLIGGRECTRACKFCHIATAKPVPLSAIAEKEHDDILAAAKAGRYDYL
                     VITSVARDDDEAGLAAHFARLTRSLNELGIRVELLVPDFHNRSEHLSVIADAHPAVIA
                     HNMETVLRLSPWVRPQAQYERSLNFFRYLRSTYPEVIRKSAFMVGLGEERAEIEQLLD
                     DMQTAGIDIVSIGQYLRPSADQAEVRKVYSDAEFAELEKSVRARGFLAWEVGPFVRSS
                     YMAHETLKKVNAEILLRRNGK"
     gene            963..1892
                     /locus_tag="PLF85_06700"
     CDS             963..1892
                     /locus_tag="PLF85_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19387.1"
                     /translation="MENKGLYRDFHRIDLSHLRQLAQEKKIESGIAQNLLAYWQRRLD
                     DKNDAIYFRYMEYYLFFESLRALFLAVFIFDMKNGMARHSYGIFPALSPAIFQSISTG
                     LNSDAGAVSFEHGGHDYSLHYIRSGYHDQEYTIAALALKDVAIEDNLKRMKYVFERYY
                     LPSSFSRDERIGNLFPEVQNLIKEWVNPALAEKQPVTFTYLYFESLTKYVGLAGEHFA
                     QDLIRELKQDVHRVLKDTDRSIILSTREILIVSVNCEEEIMRKRFANAYFHAKSLLLA
                     YQVNFFCIKEPVADMHSLWDQVSANLACKKKVS"
     gene            complement(1889..2359)
                     /locus_tag="PLF85_06705"
     CDS             complement(1889..2359)
                     /locus_tag="PLF85_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19388.1"
                     /translation="MLKTNLLLCLSLLVAPVAAIVVEGRLPGEHFREDNPPRFGALMS
                     FTSSADSAILLHGREVAPTYTIAQELKRITKSLKVPRPSLEKLGMLEGETRWLQYTFR
                     KGEGGGFLYVTRRENWILYLLVFNLRYDRLSRELPYVDRYLRQLKITGTQPAAN"
     gene            complement(2346..3698)
                     /locus_tag="PLF85_06710"
     CDS             complement(2346..3698)
                     /locus_tag="PLF85_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19389.1"
                     /translation="MKRRLMRNTAIAFTAVAFTVVVLLASQSTGAAGVRRVSAILDTS
                     SDTGITAVLPDVPGFMAKLRGGDAMRAFFDSPLGLHFLRSAPLRSAAHLHRLISLAPK
                     SWQWSLYSLITDGPVFYRSQGSSFTLVIALNKKGKAITSLLKEASVARQGDWLVIASD
                     KNTLQAQLAYMTQPAERESKLDAAFSTPSALSVLTRLDGSKPTQRGLGRALVRQALSL
                     EGESTCRLVFQPEGDSIGLEGECTIPGAKPAGQSAETITMKDFPGYVYFRRAGQHRAH
                     LIALGGFESDFGYLVPRLFFTGPTSDQKTVEFLSQAFKTRKHILEASEGAIQIRYPYP
                     YTYSDRKYDLFAPYLSANRDRFFWHSFLQDDKLRDKDLRLNPEYNFYLSVKLFPLLQN
                     SEKAIRQFDAIYSPGHFHEFRDALFKSMPALKRASVKLFAAPAQGTLRLGGAIAFAED
                     "
     gene            complement(3695..4171)
                     /locus_tag="PLF85_06715"
     CDS             complement(3695..4171)
                     /locus_tag="PLF85_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE19390.1"
                     /translation="MSVNNRKGIILCVDDEPGVLATLKEQLLSDFIGTHEVVTATSGE
                     EALAFVEAARTENEIIELIITDQMMPTMKGDEFLKRVHGILPETMKILLTGHAGLDSA
                     IKAINEGGLNRYIEKPWNAEAMRDDIKGLIAKFRQNLENQYLLNNLEARIRELTQA"
     gene            complement(4181..4810)
                     /locus_tag="PLF85_06720"
     CDS             complement(4181..4810)
                     /locus_tag="PLF85_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNE19391.1"
                     /translation="MRKVVLYFALALALCADGWKYEKSAGGAVAYTRSVPNSSFKEYK
                     VETEVEATLTQVVAVLMDVRDLPQWVDRCSEAAILKEISPTESITRTVTASPFPLKNR
                     EAISHGRLTQNPTTKVVVMESNGRFDYLPLNPKYERVHTVRGRWVLTPKPNGKVHIQM
                     IGHTDPGGVIPGAIANQFVVMIPLNTIKNLRNQIKKEKYAKAKLKYIHE"
     gene            complement(4820..5641)
                     /locus_tag="PLF85_06725"
     CDS             complement(4820..5641)
                     /locus_tag="PLF85_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNE19392.1"
                     /translation="MSVFRWALALLIPAFAVQALGAQSLPKFYNETMNFRIEWKGIRA
                     GDVTMRTRTEGDKHITINARVNSLAAVHGIYYVQGVFASKWNHVSRTPVIAFEEAYQG
                     DAYQRRKFRFSNGKVNVDKDEKKFYEHSYPHSPNLKGTWKENYDVSTKGGFQDLLGAF
                     YLVRSTGRVPRVGETLKIPVLPAGTRRSLVLKVLGREVRNDVPFFGTREIFHVRSMLV
                     DDVNKTSMGSNMFFNTETQIEMWITADADFVPVKLWTDVPYLGTAYILLENYTQP"
     gene            5909..6970
                     /locus_tag="PLF85_06730"
     CDS             5909..6970
                     /locus_tag="PLF85_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009882 - blue light photoreceptor
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNE19393.1"
                     /translation="MKRLTYISSFSRPMSNKEIDEIGERSVKNNTRDGLTGVLFCFNN
                     IFYQILEGPEDALNRCYARILKDSRHTDIFCLEVENITTRQYGDWAMKTVRLDESNDS
                     LIKPIRTMLNSLARTHYVLERYSPREVINGLQAGENPLAWTLKNNERTILFSDIFSST
                     TFAESLPSDQFERLLGVYYDIANRSVVENGGTISKLTGDGLMAYFSRGRESDALNAAI
                     EICRRLDDARSSAKADDPVHYLYAGVGLCTGLVREGNIGSAQKYDYTLLGDSVNSAAR
                     LEGVTRKVDSLIVFDESMLKGMESSPSGLRKLGLYQAKGKADNMYVYSVDYPYTRRKT
                     SLPDLKAAIQAFKTRSSAA"
     gene            7048..8697
                     /locus_tag="PLF85_06735"
     CDS             7048..8697
                     /locus_tag="PLF85_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19394.1"
                     /translation="MQATESNGFRYRWAAIGDLNAFFGLMLDNVTNLVFLAQILILVF
                     QFPADIVYTRMFPGTAFGVLVGDLIYTWMAFRLARRTQNSQVTAMPLGLDSPSTIGIA
                     FAVIGPAFLAAKSQGLNPEAAGMVAWKVGMATLIIIGIIKFVLSFFGHFVEKLIPEAG
                     LLGSLAGVGIALLGLLPLEHVFGTPVVGMVALGIIIYALVAKIKLPFNLPSVFVSVIG
                     GTIVYYALVMSGLIHAPKIDLKFVVAFPVPTLEFLGGFKEALVYLPIAIPFALLTVIG
                     GINVTASARIAGDSYRTQSVLLAEAVATLAAGITGGVAQSTPYIGQPAYKAMGGRAGY
                     TLATGLFIGLGGIFGFISLLAQVLPQAALAPILIFVGLDIVEQSYHATPKRHATAVTM
                     AILPAMPYLAKIKMLGLVAQVQGLFATVGTLSAETAAKLKPTLDVFGPAFQESWFTII
                     ALGNGFIITAMIWGAFTAKLIDRQFRMASGYLVCGAFLSLFGLMHSIKIDGDMYLPWA
                     VTPNAAGPSPFMIASAYLTLAVVILMLSYSKGAKEATLQQP"
     gene            complement(8733..9539)
                     /locus_tag="PLF85_06740"
     CDS             complement(8733..9539)
                     /locus_tag="PLF85_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19395.1"
                     /translation="MESPALVERTVSRLTAHKPVRFQINAITKVGRAQLREVLTEFFT
                     RENLDRSHILAIHYAIVEVVFNALKANVKFVAFREEIRKQLDRFKITQIEDLLQIIIE
                     ERTLREFMAVRVLPQVLKNQVQKIFDLEEKYRSGMGQRLTPEQVELIKRFRTLIRGID
                     AEVTLTITPAADSIQIRCANNVPMLQRDLDRIEASRRRHQQLQAEGRGGDFFEYENLD
                     TTESAGFGIAMVDQGFYRLGLDPFKQMKIESLNRETLVTLEYPRSVLQKH"
BASE COUNT         2069 a         2531 c         2728 g         2335 t
ORIGIN      
        1 ttgccggctg ggcctttgcc ggaattggtt tggcagacca ggtagaatcg gcatatatgt
       61 acgcgagggc tctgaagtaa tgacgcaagg cgtagcacag ccgggttcac tggcaaagaa
      121 aaaagtcgtc tggaatttcg actccgatac ttcgctgctc cgctacaacc atgtgcatac
      181 cgtttgcgaa gagtcgcgct gcccaaaccg caacgagtgc tcttctctgg gcatagccac
      241 gttcctgatc ggtgggcggg aatgcacgcg cgcctgtaaa ttctgccaca ttgccaccgc
      301 aaagccagta ccactctcgg caatcgccga aaaagaacac gatgacatcc tggcggccgc
      361 gaaggcaggc aggtatgact acctggtcat cacctctgtt gcgcgcgacg acgatgaagc
      421 agggctggca gctcactttg cccgcctgac gcgcagcctc aacgagctgg gtatccgggt
      481 ggaactgttg gtgccggatt ttcataaccg cagcgagcac ctttcggtga ttgcagacgc
      541 gcacccggct gtcattgccc acaatatgga aactgtgttg cggctttcac cgtgggtgcg
      601 accacaggcg cagtatgaac gttcgctgaa ttttttccgc tacctgcgca gtacgtaccc
      661 agaagttata cgcaagagcg ccttcatggt cgggcttggt gaagaacgtg ccgagattga
      721 acagctgctt gacgatatgc agactgccgg tatcgatatc gtttcgattg gccagtacct
      781 gcgcccttcg gctgaccagg ctgaggtgcg taaggtgtac agcgatgccg agtttgccga
      841 gctggaaaaa agcgtacgcg cgcgcggctt tcttgcatgg gaggtcgggc catttgtgcg
      901 ctcgagttac atggcacatg aaaccctgaa gaaagtaaac gctgaaattc tactgcgacg
      961 caatggaaaa taagggtctc taccgcgact tccaccgcat cgatctctcg cacctgcggc
     1021 agctggcgca ggaaaaaaag atcgagagcg gtatcgcaca gaacctgctg gcgtactggc
     1081 agcgcaggct cgatgacaag aatgacgcca tttattttcg ctacatggag tattacctct
     1141 tctttgaatc gttacgcgcg ctgtttcttg cggtcttcat ttttgacatg aagaacggta
     1201 tggcccggca cagctacggc atttttcccg cgctgagtcc cgcaattttc cagtcgatct
     1261 ccacaggcct gaacagcgat gcaggtgcag tgagctttga gcacggtgga catgactatt
     1321 cgctgcacta tattcgcagc gggtaccacg accaggaata cacgattgcc gcactggcgc
     1381 tcaaggatgt tgcgatcgaa gacaacctga agcgcatgaa atacgtcttt gagcgctatt
     1441 acctgccctc gagtttctcg cgcgatgagc gcatcggtaa tctgtttcct gaggtgcaga
     1501 acctgatcaa ggaatgggta aaccctgcgc ttgcggaaaa acaaccagtg acctttacct
     1561 atctgtactt tgagtcgctg accaagtatg ttggccttgc gggtgagcac tttgcacagg
     1621 atttgatccg cgagctcaag caggatgtgc accgggtatt aaaagacacc gaccgaagca
     1681 ttatcctttc gacgcgtgag attttaatag tctcagtgaa ctgtgaagaa gagatcatgc
     1741 gcaagcggtt tgcgaatgcg tactttcatg cgaaaagcct tttgcttgct taccaggtga
     1801 acttcttttg tatcaaggaa ccggtcgccg atatgcacag cctctgggac caggtcagcg
     1861 ctaatctcgc ctgcaagaag aaagtaagtt aattcgccgc tggttgcgta ccagtgattt
     1921 tgagctggcg caggtagcgg tcgacatacg gcaattcgcg cgagagccgg tcatacctga
     1981 gattgaacac gagcaggtag agaatccagt tttcccgccg cgtcacgtag agaaatccac
     2041 cgccttcgcc ttttcggaaa gtgtactgta gccagcgcgt ttcgccctcg agcataccga
     2101 gtttttccag cgatgggcgc ggaaccttga ggcttttggt aatacgtttc agctcctgcg
     2161 cgatggtgta tgtgggtgca acttcgcggc catgcagaag gattgcagaa tcggcgctcg
     2221 aggtgaagct catcagcgca ccaaagcgcg gtggattgtc ttctctgaag tgttcgccgg
     2281 gcagccggcc ttcgacgact attgcggcaa ctggcgcaac taaaagagac aggcagagaa
     2341 ggagattagt cttcagcaaa cgcaatcgct ccgccaagcc gcagcgtacc ttgtgctggc
     2401 gcggcaaaaa gttttaccga ggcgcgttta agcgctggca tgctcttgaa gagtgcatcg
     2461 cggaattcat ggaaatggcc gggcgaatag atcgcatcaa actgcctgat ggccttttcg
     2521 gagttttgta acaacggaaa cagtttcaca gacaggtaaa agttgtactc gggattcagc
     2581 ctcaggtctt tgtcgcgtag tttgtcgtcc tgaagaaaac tatgccagaa aaagcggtct
     2641 cggttagctg acaggtacgg cgcaaacagg tcgtatttgc ggtctgaata ggtgtatggg
     2701 tacggataac gaatctgtat agctccctcg ctggcttcga gaatgtgctt gcgggtcttg
     2761 aacgcctggc tcaggaactc gacagtcttc tggtcactgg tgggcccggt aaaaaatagc
     2821 cgcggtacga ggtaaccgaa atccgattcg aacccaccca gcgcaatcag gtgcgccctg
     2881 tgctgaccgg cgcggcgaaa gtagacatag ccgggaaaat ctttcatcgt gatggtttcg
     2941 gcagactggc ctgcgggctt cgcgccgggt attgtgcact cgccctcgag gccgatactg
     3001 tcgccctcgg gctgaaaaac aaggcggcag gtgctttcgc cttcgagact gagtgcctgc
     3061 cgcaccagcg ccctgccaag accgcgttga gtgggtttgc tgccatcgag gcgggtcagc
     3121 accgaaagag cagagggagt agagaaggct gcgtcgagtt ttgattcgcg ttcggccggt
     3181 tgagtcatat aggcgagttg cgcctgcagc gtgttcttgt ctgacgcaat gacgagccag
     3241 tcaccctggc gcgcaactga agcttctttc agtaatgagg taattgcctt gcccttctta
     3301 ttgagcgcaa tcaccagcgt aaagctactg ccctgcgaac ggtagaatac cggcccatcg
     3361 gtgatgagtg agtacaggct ccactgccat gatttcggtg cgagggagat caggcggtgc
     3421 aggtgcgcgg cgctgcggag tggcgctgag cgcagaaagt gcaggccgag cggcgagtca
     3481 aagaaagcgc gcatggcgtc gccgccgcgc agttttgcca tgaatccggg cacatcgggt
     3541 agtactgcgg taatgcccgt atcagagctg gtatcgagaa ttgccgatac acggcgtacc
     3601 cccgctgcgc cggtcgactg tgaggccaac agtacgacaa cagtgaatgc aaccgccgtg
     3661 aatgcgatcg cggtattgcg catcaatctg cgcttcatgc ctgggttagc tcgcgtatgc
     3721 gcgcttcaag gttattgagc aggtactggt tttccaggtt ctgtctgaac ttggcgatga
     3781 ggcccttgat gtcgtcccgc atcgcttcgg cattccaggg cttttcgatg tagcggttca
     3841 ggccgccttc gttgatcgcc ttgattgccg agtcgagacc ggcgtgaccg gtgagcagaa
     3901 ttttcatcgt ttcgggcagt atgccgtgaa cccgcttgag gaattcatcg cccttcatcg
     3961 ttggcatcat ctggtcggtg ataatgagtt caatgatttc gttttccgtg cgcgccgctt
     4021 cgacaaaggc gagcgcctct tcacccgaag ttgcggtgac gacttcatgt gtaccgataa
     4081 aatcagagag caattgttcc ttgagggtag ccaggacgcc gggttcgtcg tctacacata
     4141 gtatgattcc cttgcggttg tttacagaca ctgggtcccc ctattcatga atatacttca
     4201 gcttcgcttt ggcatacttt tctttcttta tctggttgcg caagttttta atcgtattga
     4261 gcggaatcat cacaacgaac tggttggcga tcgcgccggg tatcactccg ccggggtcag
     4321 tatgcccaat catctggata tgcactttgc cgttgggttt cggcgtcagc acccaccggc
     4381 cgcgcactgt gtgaacgcgc tcgtatttcg ggttaagcgg caggtaatca aaacggccgt
     4441 ttgattccat gacgacaact tttgtggtgg ggttctgcgt caggcggcca tgcgaaatgg
     4501 cctcgcggtt cttgagagga aaaggcgaag cggtgaccgt gcgggtaatg gattcagtgg
     4561 gggagatttc tttgagtatc gcggcctcac tgcagcgatc gacccactgt ggtagatcgc
     4621 gtacatccat taagacagcc acaacctgtg tcagggtagc ttccacttcg gtttcgactt
     4681 tatattcttt aaagctagaa ttaggcaccg atcgggtgta cgctacagca ccgccggcag
     4741 atttttcgta tttccagccg tcggcgcaaa gtgctagcgc gagggcaaaa tataagacaa
     4801 cttttcgcat tgtaccttat tacggctgcg tgtagttttc gagcagtatg taagcagtgc
     4861 caagataagg tacgtcagtc cagagtttga cgggcacgaa atctgcgtca gccgtgatcc
     4921 acatttcaat ttgtgtttct gtgttaaaga acatattgct gcccatgctt gtcttgttga
     4981 cgtcgtcaac cagcatcgag cggacatgaa aaatctcccg tgtgccgaag aagggcacgt
     5041 cgttacgcac ttcgcggcct agcaccttga gtaccagcga gcggcgtgta ccagcgggaa
     5101 gcaccggtat cttcagggtt tcacccaccc gtggcacacg gccagtactg cgcacgaggt
     5161 aaaaagcccc caggaggtcc tggaaccccc ccttggtgct cacgtcatag ttttctttcc
     5221 atgtaccttt gagattaggg gagtgcgggt agctgtgctc gtagaatttc ttttcgtctt
     5281 tgtcgacgtt taccttgccg ttgctgaaac gaaacttgcg gcgctggtag gcatcgccct
     5341 ggtaggcttc ttcaaaagca atgaccgggg tgcgggagac atggttccac ttgctggcga
     5401 agacgccctg cacatagtag atgccatgaa cggcggccag tgaattgacg cgcgcgttga
     5461 tcgtgatgtg cttatcgcct tcggtgcgcg tgcgcatggt cacgtcgccg gcgcgtatac
     5521 ctttccactc aatgcggaag ttcatggttt cattgtagaa cttcggcagg ctctgcgcgc
     5581 ccagcgcctg gactgcaaac gccggaatca agagcgctag cgcccaccga aagactgaca
     5641 taatcgcgaa tggtgtttcc ggttcgccgg tcaacacatt cttcaggctc ctttgctgca
     5701 tttcttaatt agacgcaggc aaaagaaaag gcggagtgtt tttttgtgcc tgtctttacc
     5761 gaacaactta gctccgcgcc agcaacaggt gtctgcctaa gcggtgaatg cgacataatc
     5821 aggtcaaaca caggtgttgc tgtttttagt gtcaaaggtt attaaaaaag attggacgat
     5881 tgtcgtatgt ttattgtata cttatgacat gaaaagatta acctatatca gcagtttctc
     5941 ccgcccgatg tcgaataaag agatcgatga aattggcgaa cgttcggtga aaaacaacac
     6001 ccgtgatggc ctcactggcg tcctgttttg tttcaacaac atcttctacc agattctcga
     6061 agggcccgaa gatgcgctca accgctgcta tgcgcgcatt ctcaaagaca gccgccatac
     6121 cgatattttc tgcctcgagg tagaaaatat caccacccgg cagtacggtg actgggcgat
     6181 gaaaaccgtg cgcctcgacg aatcgaacga ttcgctgatc aagccgatac gcacgatgct
     6241 gaactcgctc gcgcgcacgc actatgtgct tgagcggtat tcgccgcgtg aagtgatcaa
     6301 cgggctgcag gcgggtgaaa acccgctcgc ctggactctc aagaacaacg agcggactat
     6361 cctcttttcg gacatcttct cttcgaccac gtttgccgag agcctgccca gcgaccagtt
     6421 cgaacgcctg ttgggagtgt attacgatat cgcaaaccgc tcggtagtag agaatggcgg
     6481 taccatttca aagctcaccg gcgacggcct gatggcgtat ttctcgcgtg gccgtgagtc
     6541 tgatgcgctg aatgcggcga ttgagatctg ccgtcgtttg gacgatgctc gcagttctgc
     6601 gaaggccgac gatcccgtac actacctcta tgccggggtc ggcctttgta ccggtttggt
     6661 tcgcgaaggt aatatcggtt ctgcgcagaa gtacgattat acactgctcg gcgactcggt
     6721 gaactctgca gcgcgtcttg aaggcgtgac gcgcaaggtg gactcgttga tcgtgttcga
     6781 tgaatcgatg ctaaaaggca tggaatcgtc gccaagcgga cttcgcaaac tggggctcta
     6841 ccaggcgaaa ggcaaggcag acaacatgta tgtgtactcg gtagactatc cgtacactcg
     6901 caggaaaact tcactgccag atctgaaagc cgctattcag gcatttaaga caagaagctc
     6961 agcagcttaa gcggcttatg aacgggggtt tataaccccc ggattatttt agattgaccc
     7021 gcgcttttgc acctcgcttg ctatcccatg caagcgactg aatctaacgg tttccgttac
     7081 cgctgggcgg cgatcggcga cctcaatgcc ttttttgggc tgatgctcga caatgttacc
     7141 aaccttgtct ttcttgcgca gatactgatc ctcgtcttcc agtttcctgc cgatatcgtc
     7201 tacacgcgca tgtttcccgg cactgcgttt ggcgttctcg tgggcgattt gatttacacc
     7261 tggatggcgt ttcgtctcgc gcgccgtacg cagaactcac aggtcacggc catgccgctg
     7321 ggccttgatt caccttcgac gataggtatc gcttttgcgg tcatcggccc ggcatttctc
     7381 gccgcaaaat cacagggcct caaccctgaa gctgccggta tggtcgcatg gaaggtgggt
     7441 atggcgacgc tgatcattat cggtattatt aagttcgtgc tctcgttctt cggccacttc
     7501 gtcgagaaac tgattcccga agcaggtctc ctcggttcgc tggccggcgt gggtattgcg
     7561 ctgctcggtt tgctgccgct cgaacacgtg ttcggaaccc ccgtggtcgg tatggttgcg
     7621 ctgggtatta tcatctatgc actggtcgca aagatcaaac tcccctttaa tctgccgagc
     7681 gtttttgtct cggttattgg tggtacaatc gtctattacg ctctcgtgat gtcgggtctc
     7741 attcatgcac cgaagatcga tctgaagttt gtggttgctt ttcctgtacc gacgctcgag
     7801 ttcctgggag gatttaagga agcgctggtt tatctcccca ttgcgattcc atttgccctg
     7861 cttaccgtga tcggcggtat taacgtaacg gcgagcgcgc gcattgcggg cgactcatac
     7921 cgcacgcaga gcgttctgct tgctgaagcc gtggcgacgc tcgcggccgg cattaccggc
     7981 ggtgtagcgc agtcgacgcc gtacattggg caaccggcgt acaaggcgat gggtgggcgc
     8041 gcaggctata cgctggcgac tggcctcttc attggcctgg gcggtatttt cggttttatc
     8101 tcgctgctgg cgcaggtgct gccgcaggca gcgctggcac caattctgat ctttgtggga
     8161 ctcgatatag tggagcaatc gtaccatgca acacccaagc gccacgcaac cgcggtgacg
     8221 atggcgattc tgccagcgat gccgtacctg gcaaagatca agatgctggg gctcgtcgcg
     8281 caggtgcagg gtctttttgc gacggtcggc acgctttcag cagagacagc ggcaaaactg
     8341 aagcccactc tcgatgtctt cgggcctgcg ttccaggaat cgtggtttac tatcatcgcc
     8401 ctcggtaatg gatttattat cactgcaatg atctggggag cgtttaccgc gaagctcatc
     8461 gatcgccagt tccgtatggc gtcagggtac ctggtctgcg gtgcattcct ctccctgttt
     8521 gggctcatgc actcgatcaa gatagacggc gacatgtacc tgccatgggc ggtaacgccg
     8581 aacgcagccg gcccttcgcc gttcatgatt gcatcggcgt acctgacgct ggcagtggta
     8641 attcttatgc tttcctactc aaagggagca aaagaggcta cactccagca accgtgacgc
     8701 gcggcgggcc cgcagcccgc gcgctgattg cattaatgtt tctgtaaaac cgaccgcggg
     8761 tactcgagag tcacaagtgt ctcgcggttg agcgattcga tcttcatctg tttaaaagga
     8821 tccaacccca ggcggtaaaa accctggtcg accatggcaa tgccaaagcc ggccgattcg
     8881 gtagtatcga gattctcata ctcaaagaaa tccccaccgc ggccttcggc ctgcagctgc
     8941 tggtggcggc gccggctggc ttcgatacgg tcgaggtcgc gctgtagcat gggtacgttg
     9001 ttagcacagc gaatctgaat cgaatcggca gcgggagtga tagtcagtgt cacctctgca
     9061 tcgatacccc gaatcagcgt gcggaagcgt ttgatgagtt caacctgctc aggagtcagc
     9121 cgctggccca tgcccgagcg gtatttttct tccaggtcaa aaattttctg cacctggttc
     9181 ttgagtacct gcggcagcac cctgacagcc atgaattcgc gcagggttct ttcttcaatg
     9241 atgatctgta gcaggtcttc gatctgcgta attttgaagc ggtcgagctg cttgcgtatc
     9301 tcttcacgga aagcgacgaa cttgacgttg gctttgagcg cgttgaagac gacttcgacg
     9361 atggcgtagt gtatcgcgag tatatgagaa cggtcgagat tttcgcgagt gaagaattcg
     9421 gtcaaaacct cgcgcagctg cgcccggcct acctttgtga tagcgttgat ctggaagcgg
     9481 acgggtttat gcgcagtaag gcggctaacg gtgcgttcaa ccagcgcggg agattccatg
     9541 cataattatc gtatgcaggc gttcacaaac taaagcggtg tttcacgtga aacaccgctt
     9601 tggtacctat tagcgcttca ggcccaggag ttcctgcagc atctggtcgg cggtagtgat
     9661 gct
//
LOCUS       DAOQJF010000095         9455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3842_length_9455_cov_5.570638, whole genome shotgun sequence.
ACCESSION   DAOQJF010000095 DAOQJF010000000
VERSION     DAOQJF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3842_length_9455_cov_5.570638"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLF85_06745"
     CDS             complement(<1..262)
                     /locus_tag="PLF85_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19396.1"
                     /translation="MQETINKTAGEIAAECAQGYRLRGLVSGETIVVKKISGTEDCGP
                     GDLVFVSDAKLVAETLARKPAAMVIHFKEAEKLGAQTSTAIIA"
     gene            339..557
                     /gene="infA"
                     /locus_tag="PLF85_06750"
     CDS             339..557
                     /gene="infA"
                     /locus_tag="PLF85_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNE19397.1"
                     /translation="MAKEEAISVNGIIKKALPNAMFLVELENGHEILGHISGKMRKHY
                     IRINPGDRVSVELSPYDLTRGRITYRVA"
     gene            complement(603..1946)
                     /locus_tag="PLF85_06755"
     CDS             complement(603..1946)
                     /locus_tag="PLF85_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNE19398.1"
                     /translation="MVPKNIHIIGAGGVGMSGLALLARRMGAVVTASDQSDSPYLRKL
                     AEKGITTWVGSQPERMPASAHVFYSTAIKPEDSERTHAERSGMPCESRHQLLNLITRN
                     YFTIAIAGCHGKTTTSAWTARLLERAGFDPTALIGGTVPEWNSNYREGHGTMQGKPLL
                     VIEADESDRSFLSIDTNVAMVTNIDLDHTDVHASLEALTLDFLGFLAVAQAQGGWIHL
                     SKECPQLLQEQLSKQDQAIWINIAVDTVAHAVIYENKKYPVGLAGTHNLLNATLVLQL
                     ALKLGIDHEILADTLREFGGVNRRMQTIATFPEKKLVVIDDYAHHPHEVEATLSALAA
                     RYDRLLIFWEPHRLSRFNHFFAEFDAVLRRYASGHALFVLPIFASGDKLDEYPQTEER
                     FAFFKNQPYVYVGDLAGYEQTAGEWTGSNNAAVFMGAGKSSEYAHAYAHWLQAET"
     gene            complement(1966..2289)
                     /locus_tag="PLF85_06760"
     CDS             complement(1966..2289)
                     /locus_tag="PLF85_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19399.1"
                     /translation="MRKIGKRILQALAIAFAGVIIFLAANYKHTAAFPGIISSFYSKE
                     FCSCYFVTEGTEEQCHNFARQWVPLQSFALDKEKRTVTAKGLFVTTTARYTGDRYGCI
                     LEPWQ"
     gene            complement(2289..3509)
                     /locus_tag="PLF85_06765"
     CDS             complement(2289..3509)
                     /locus_tag="PLF85_06765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE19400.1"
                     /translation="MKKHYLASVALLVLVACGKKVLAPAQVREALPATFEQQARTNTY
                     PGAEWSELSAEKAGINREKLRNLEQYLFPEKIDEKTRQGIRTDAFVIVRGGNIVFEKY
                     AREFHRNRKHLAWSVTKSFANTLAGMAVADGKIRVEDSAAKYYPALAGDGKEAILIDH
                     LLRMSSGLYWSEGYESSPLKSSVVAMLYTRGRNDMAAFAAAQDLIYQPGTHWYYSSGT
                     SNILTALVRNAYNSPADPKQPGDKFEDLVFNRLFKSIGMRYTTFERDGAGTPVGSSYL
                     YTTARDLARFGYLYLHDGVWQGRRLLPEKWVRYTTTMAPAYYTTELKPEDAKDNPGAQ
                     WYINVGIPERGQQPPWPDAPADTFAALGHWGQSVYVIPSLDMVAVRFGDDRDGSFNKN
                     RYLGLLKNILERAP"
     gene            complement(3545..4042)
                     /locus_tag="PLF85_06770"
     CDS             complement(3545..4042)
                     /locus_tag="PLF85_06770"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNE19401.1"
                     /translation="MRPLRPLFRRSAAKDELERQAERLRNRDWIEMLRVVHPAQAAQA
                     EQEYLLARFRDDWAELVNPLLAQHSQPAEVNGSTLVVHCDHNTFANELMLIASVVEKK
                     IHARYGYTLRIRAKATQRLAWPKQEPGAEPARVTSTPETPEKKTPVDVLIAELSDYQA
                     SSSTK"
     gene            complement(4059..6794)
                     /locus_tag="PLF85_06775"
     CDS             complement(4059..6794)
                     /locus_tag="PLF85_06775"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE19402.1"
                     /translation="MARKKAPGKPTNKKPTPAKKKYPVRKDAALSTSAKLKQGDPTSE
                     AELSALFEATQTTDDKQREAAPVKSAPSAELIRPRRRYGVWAALLLIILVAGGYIWLR
                     ARLSGSLVPDEGLLTVQGLTADVEIRRDSLGVPHIKAQNLNDMAFGAGFAMAADRDFQ
                     IEFLKRMAYGRLSEVLGKETVGVDMYMRSLSFAALTRQNFERLSPKMQGMLKAYAAGV
                     NAKRRAYPKTQAEYLILSIATEEWQPQDSLALYQLFSFLLASNHIEELTFLKLAAKLG
                     VEKAAWLFPVYADEPLPFEEAAHLKDLDWKKISANEPVLGAALHDAASRWQALIPSLP
                     ASNNWIVAPSRAKNGKSLLANDTHLQMSVPALWYMMNLECPEYRAAGVALPGTPVVAL
                     GTNGTIAWGATMVMADNQDIFLEQLREQGGRQQYLYKGEWLDASTSEEEIQVRWSSPR
                     KFRKIRTRHGVLLNEALLNAFPDKHLALGLRSDYGLAYKTSVGASETTLEGIFTMASA
                     KTMRDARSALDRVTGIYLNVVYADEKNIGWLATGRYPARKGVSGLFPRIGWTGENEWQ
                     GYYAPAENPSVLNPKEGYWATANHRIWDDSSELWVSSSWYGADRADRAKELLAAGKQH
                     TAEDMAKFQGDVVSHTARKTKELLFSGEFSAELKAAIDLLPPSEKSRAEGALSLLKNF
                     DGSLNKDSAAAAVYENFVSAMAVLTFADELGGHGSPLWENFQGLNKRSYNAVQDHLLA
                     RADSPFWDNTQTPEKQESKAMIFASALAGSIEESEKQMGKARDKWAWGKIHEYYWQHQ
                     FTKKAGYLSFYLNRGPHAAGGDLHTLNQAGNLWGDSHNVWLIPAMRFIVDFSADEPAQ
                     LIVHMGVSGNAESPHYADMVPLFTEVRNHSLPLKANNAEKQYNRKFVLKPGGAAR"
     gene            complement(6817..8061)
                     /locus_tag="PLF85_06780"
     CDS             complement(6817..8061)
                     /locus_tag="PLF85_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE19403.1"
                     /translation="MLKKKPLLLVEDDPVQQALIERMLSFMPYSLTVVATAQEALDAF
                     QKVGHEVVLCDINLPGELSGVDVLRRLKETPHPPVVVMLTGDSAIDNVIETMRLGAYD
                     YLVKPVQSAELDDVIGRAFARSQSEVVARIAERERAEYYEKRLASRQVADQLVRRQND
                     KFARALFGNMFTSFSQGRGVGSLTTLVSMLGGAQLTEDKQNYLVPKDILDLINDNQDA
                     VNAMIEMFSELHLLVSQDLVLHDVSLGEFHKIVSDLIFELNPVIEMRRHAVVLSELPP
                     RFSGEVLKMNWEFMRKALRELLINALKFSEDESTVTVLIEYKFTRVLVTVLNEPLRTD
                     SDYVQGIPEEYRKLVFEPFFRISRVVREQYRTLEYGLGLTLVDKIVYMHHGKIRCTSL
                     QNFLGGEGNATDLVAFEIELPI"
     gene            complement(8173..9060)
                     /locus_tag="PLF85_06785"
     CDS             complement(8173..9060)
                     /locus_tag="PLF85_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19404.1"
                     /translation="MDTKKQRKNLEKLTPAEYSSLLKLYLDEDKQPQAIKQASENLSA
                     LEMLVQFETMVAVSKRHQATTQPASFQVALHEVVAIAEKFFSVEDPRAMEKDLQASRI
                     WNAIKASPADFALFQDTLAFLFTWGERPMLPTPAYLKQIVMNRIYAEAPQDGESTIVI
                     RLRDGLRLIAGHIRDLYNITNPDDMVAVRSGSDQASAHAGNPTGALQFMTSEANQGDL
                     VYQVVKDGADTVMLTVKLQNYRPRPKFINLRRQGRLLQSMPLRDDFAWFSQLAVGKYE
                     IELQNAGSVSGKRVDIHIV"
     gene            9156..>9455
                     /locus_tag="PLF85_06790"
     CDS             9156..>9455
                     /locus_tag="PLF85_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNE19405.1"
                     /translation="MLRLITISISHYVEKVKWALELSGIDYKEESHIPGLHAAVTLWH
                     TRGRHRSTPVLIDGNQVVPDSTAILRHLADKYDQRWLYPAPNALELEERFDTAVGP"
BASE COUNT         1957 a         2700 c         2627 g         2171 t
ORIGIN      
        1 tggcaatgat cgccgtcgat gtctgtgcac cgagcttttc ggcctcttta aaatgaataa
       61 ccatggcagc cggcttgcgt gccagcgttt cagctaccag ctttgcatcg ctcacaaaga
      121 ccaggtcgcc cggcccgcag tcttctgttc ccgagatctt ctttacgacg atcgtttcac
      181 cgctcacaag gccgcgaagc ctgtaaccct gcgcgcattc tgccgcaatt tcacccgcgg
      241 ttttgttaat tgtctcttgc atcgaccatc atgccaaacc ccgacctgtt gactgccaaa
      301 gtattcgacg ccctgtgtca aacgggcaga atgccgcaat ggccaaagaa gaggcaatca
      361 gcgtcaacgg aatcatcaag aaggctctgc ctaacgcgat gttccttgtc gaactcgaaa
      421 acggccacga gatcctcgga cacatctccg gcaagatgcg caagcactac attcgtatca
      481 acccaggcga tcgggtgtct gtagaactgt caccttacga cctgacacgc ggccgcatca
      541 cttaccgcgt cgcttaatca acccaacctg ctggtatacc agcttttttt cctgatttct
      601 tgctacgttt ctgcttgcag ccaatgcgcg tatgcatgcg catactctga actcttgcct
      661 gctcccatga atactgcggc gttgttgctg cccgtccact cacctgctgt ctgttcgtag
      721 ccagcgagat cgccaacata cacataaggt tgatttttga agaacgcaaa tctctcttca
      781 gtttgcggat actcatccaa cttatcccct gaggcaaaga tgggcagcac aaagagcgca
      841 tgtcccgatg cataacggcg caggacagca tcaaattccg caaagaagtg gttaaagcgg
      901 ctcaggcggt gcggctccca gaaaatcagc aaccggtcgt accgtgcggc cagcgctgac
      961 agtgttgcct cgacttcatg aggatggtgc gcatagtcat caatgaccac gagttttttt
     1021 tccgggaaag tcgcaatggt ctgcatgcgc cggttaacgc cgccaaattc gcggagtgta
     1081 tcagcgagta tttcgtggtc tatacccagc tttaatgcca actgcaatac cagcgtcgcg
     1141 ttcagcaggt tgtgcgtccc ggcgagacca acaggatact ttttattttc gtatatgacc
     1201 gcgtgggcaa ccgtgtcgac tgcaatattt atccatatcg cctggtcttg ttttgaaagc
     1261 tgctcctgca acagctgcgg acattctttt gaaagatgaa tccatccgcc ctgcgcctgc
     1321 gccacagcaa gaaagccgag aaagtccagc gtcagcgctt cgaggctcgc gtgcacgtcg
     1381 gtgtggtcga gatcgatgtt ggtgaccatc gcaacattgg tatcaatcga cagaaaactg
     1441 cggtccgatt cgtcggcctc gatgacaagc agtggcttac cctgcatggt gccgtggcct
     1501 tcgcggtagt tcgagttcca ttcgggcacc gtgccgccaa taagcgcagt cgggtcgaac
     1561 ccggcccgtt cgagcaggcg agcagtccac gcgctcgttg tggttttacc atggcatccc
     1621 gcgatggcga ttgtgaagta attgcgcgtg atcaggttga gcaactggtg acgggattcg
     1681 cacggcatgc cgctgcgttc tgcgtgcgta cgttcagagt cctcgggctt gatggctgtg
     1741 gagtagaaca cgtgcgctga agcgggcata cgttcgggct gcgagcccac ccacgtggtg
     1801 ataccttttt ctgccagctt gcgcaggtaa ggcgaatcgc tctggtctga agcagttacg
     1861 acagcgccca ttcgcctggc cagcagtgcc agcccgctca tgccgacgcc gcctgcgcca
     1921 atgatgtgaa tattctttgg aaccaaggtt attttaccgg agcatttact gccacggctc
     1981 gagaatacaa ccgtaccggt cgccggtata acgcgcggtc gtcgtgacaa agagtccctt
     2041 tgccgtaact gtccgcttct ccttatcgag cgcaaatgac tgtagcggca cccactgccg
     2101 cgcgaagttg tggcactgct cttcggtgcc ttcagtcaca aagtaacagg agcaaaactc
     2161 tttgctgtag aaggaagaaa taatgccggg gaacgccgca gtgtgtttgt aattcgctgc
     2221 gaggaaaatt atcacccctg caaaggcgat agccagtgcc tgcagaattc gtttgccgat
     2281 ttttcgcatc acggtgccct ctcgagaata ttcttcagta aaccaaggta gcggttcttg
     2341 ttgaatgatc cgtcgcggtc gtcgccgaac cttacggcaa ccatatcgag tgaaggtatc
     2401 acatagacag actgtcccca gtgcccgagc gccgcgaagg tgtcggcagg agcatcgggc
     2461 cacggcggct gttggccacg ctcgggaatt cccacgttga tgtaccactg tgcaccgggg
     2521 ttatccttcg cgtcttcagg tttaagttcg gtggtatagt atgcgggcgc catggtggtc
     2581 gtgtagcgta cccacttctc gggcaagagc cgccgaccct gccacacacc gtcatgcagg
     2641 tagaggtagc caaagcgtgc cagatcgcgg gccgtggtat agaggtacga tgacccaacc
     2701 ggggtacccg ctccgtcgcg ttcaaaggtc gtgtagcgca tgccgatcga cttgaacagg
     2761 cggttaaaga cgagatcttc aaacttgtcg ccgggctgct tgggatcagc gggggagtta
     2821 taggcgttac gcaccagagc cgtcaggatg ttgctcgttc cgctcgaata ataccagtgt
     2881 gtgcccggtt ggtagatgag gtcctgcgcc gccgcgaagg ctgccatgtc attgcggccc
     2941 cgcgtgtaca acatggcgac gaccgacgac ttgagcggcg agctctcgta gccttcagac
     3001 cagtagagcc ctgaactcat gcgcagcagg tgatcgatga gaatggcttc tttgccatcg
     3061 ccggccagtg ccgggtagta tttcgccgcg ctgtcctcaa cgcgtatctt tccgtccgca
     3121 actgccatgc cggccagcgt attggcaaaa ctttttgtga ctgaccaggc gaggtgcttg
     3181 cggttgcggt ggaactcgcg agcgtacttc tcaaaaacaa tgttgccccc gcgcacaatc
     3241 acaaacgcgt cggttcgaat accctgcctc gtcttctcat cgatcttctc gggaaaaagg
     3301 tactgctcaa ggttgcggag tttctcgcgg ttgatgcccg ccttctcagc ggaaagttcg
     3361 ctccattcgg cgccggggta agtattggtg cgcgcctgct gttcaaacgt tgcaggcaaa
     3421 gcctcgcgca cctgcgccgg ggccagaacc ttcttgccgc aggcaaccag gacgagaagt
     3481 gccacggacg ccaggtagtg ctttttcacg ttgccagact tgtgcaaacc agtactcctg
     3541 caatttattt tgtgctgcta ctggcctggt agtcgctcaa ctcagcgata aggacatcga
     3601 caggggtctt cttttccgga gtttccgggg tgcttgtcac tcgagcgggc tctgcgccgg
     3661 gctcttgctt tggccaggcg aggcgctgcg tggcttttgc gcgtattctc aatgtatagc
     3721 cgtaacgcgc atggatcttt ttctcaacga ccgaagcgat gagcatcagc tcgttggcga
     3781 atgtattgtg atcgcagtgc actacgagag tgctgccgtt tacttcagct ggctgcgagt
     3841 gttgcgccag caatggattt accagctcgg cccagtcgtc tcggaagcgc gccagcaggt
     3901 attcctgttc tgcctgcgcc gcctgcgcgg gatgcacgac gcgcagcatc tcgatccagt
     3961 cacggttgcg cagacgctcc gcctgccgct cgagctcgtc tttagcggca ctgcgacgaa
     4021 acagcgggcg aaggggacgc atgacggtcc aggcgcgatt accgggctgc gccgcccggc
     4081 ttcaggacaa acttcctgtt gtactgcttt tccgcgttat ttgccttcag cggcagcgag
     4141 tggttcctga cctcggtaaa cagcggtacc atgtcggcat aatgcggtga ttctgcattg
     4201 cccgaaactc ccatgtgcac gatcagttgc gcgggctcgt cggctgaaaa atcgacgata
     4261 aaacgcatcg cgggtattag ccagacattg tgtgagtcgc cccagaggtt gcccgcctgg
     4321 ttgagggtat gcaggtcgcc acccgccgca tgtgggccac ggttgagata gaacgaaaga
     4381 taaccggctt tttttgtgaa ctggtgctgc cagtagtact cgtgaatttt cccccatgcc
     4441 cacttgtcgc gcgctttgcc catctgtttt tcagactctt caatcgaacc cgcaagtgcc
     4501 gatgcgaaaa tcatcgcctt actttcctgt ttctccgggg tctgcgtgtt atcccagaac
     4561 gggctgtcag cgcgcgccag caggtggtcc tgtactgcgt tgtaggagcg tttgttgagt
     4621 ccctggaagt tttcccagag cggcgaacca tggccaccca gctcgtcggc aaaggtcagg
     4681 accgccatcg ccgaaacaaa attttcatac actgcggccg cagctgaatc tttgttcagg
     4741 ctcccgtcga aatttttcaa cagcgagaga gctccttcag cgcggctttt ttccgacggg
     4801 ggcagcaggt caatcgccgc cttcagctcg gcagaaaatt cgcctgaaaa cagcagctcc
     4861 ttcgttttgc gcgcggtgtg cgaaaccacg tcgccctgga atttcgccat gtcttcggcc
     4921 gtgtgctgtt tgccggcagc caggagttct tttgcacgat cggcgcggtc ggcgccatac
     4981 cacgatgagg aaacccagag ttcgctcgag tcatcccaga tacggtggtt tgcggttgcc
     5041 cagtaacctt ctttggggtt cagtaccgag ggattttcgg ccggagcata ataaccctgc
     5101 cattcgttct cgccagtcca gccgatgcgc ggaaagagac cgctcacacc cttacgcgcg
     5161 ggataccttc ccgtcgcgag ccagccaatg tttttttcgt ctgcatatac cacattgaga
     5221 tagatgccgg taaccctatc aagagccgaa cgtgcatcgc gcatagtctt cgccgaggcc
     5281 attgtgaaaa ttccttctaa cgtggtttcg ctcgcgccga ccgaagtctt gtaagccagg
     5341 ccgtagtcgc tgcgcaggcc taatgcaagg tgtttatcgg gaaatgcatt cagcagcgct
     5401 tcattcagca gcaccccgtg acgggtacgg atcttcctga acttgcgcgg tgacgaccag
     5461 cgtacctgta tctcttcttc gctcgtgctc gcgtcgagcc actcgccctt gtagaggtac
     5521 tgctgcctgc caccctgctc gcgcagctgc tcgagaaaga tatcctggtt gtcggccatg
     5581 accatcgtcg caccccaggc gatggtaccg ttggtaccga gcgccaccac cggcgtaccc
     5641 ggcagtgcta cgcccgcggc acggtactcg gggcattcga ggttcatcat gtaccacagc
     5701 gccggcacag acatctgcag gtgcgtatca ttcgcgagca acgacttgcc attcttcgcg
     5761 cgactcgggg caacgatcca gttgtttgaa gctggcaacg acggtatcag cgcctgccag
     5821 cgcgaagcgg catcgtgcag cgctgcgccc agtaccggtt catttgctga tatcttcttc
     5881 cagtcgaggt ccttgaggtg tgcagcctct tcaaacggca gcggttcgtc ggcgtagacc
     5941 gggaaaagcc atgccgcttt ttcgacgccc agcttcgcgg cgagcttgag gaatgtcagc
     6001 tcttcaatat ggttacttgc cagcaaaaag gagaacaact ggtacagcgc cagtgaatcc
     6061 tgtggttgcc attcttcagt ggcgatggag agaatgaggt actcggcctg cgtcttcggg
     6121 tacgcgcgcc gcttcgcatt cacacccgcg gcataggctt tcagcatacc ctgcatcttc
     6181 ggtgacaggc gttcaaagtt ttggcgcgtg agtgcagcaa aagacaacga ccgcatgtac
     6241 atgtcgacgc ccacggtctc tttacccagt acctcagaaa gccggccata cgccatgcgc
     6301 ttcagaaatt caatctggaa atcacggtct gcagccatcg cgaaaccggc gccgaaggcc
     6361 atatcgttca agttctgtgc cttaatgtgc ggcaccccga gactgtcgcg gcggatttcg
     6421 acatctgctg tcagcccctg gaccgtgagc agaccttcgt caggtacaag actaccagac
     6481 agcctcgcac gcagccaaat gtagccgccg gcaaccagga taataagcaa aagcgctgcc
     6541 caaacgccgt aacggcgtcg cgggcgaatg agttcagccg aaggcgcgct ttttaccggc
     6601 gccgcctcgc gttgcttgtc gtcggtggtt tgcgtagcct caaacagcgc gctgagctcg
     6661 gcttcagaag tggggtcgcc ctgctttagc tttgccgatg tagaaagagc tgcgtctttc
     6721 ctgactgggt atttcttttt tgctggagta ggtttcttgt tggtcggttt tcccggtgct
     6781 ttcttacgcg ccatcaaggg agtgcgtaac caaaagtcag attggcaact ctatttcaaa
     6841 tgccacgaga tcggtcgcat tgccctcgcc acccagaaaa ttctgcaggc tcgtgcagcg
     6901 aattttgccg tggtgcatat agacaatctt gtcgaccagc gtcagtccta acccgtattc
     6961 gagggtacgg tactgctcgc gtacgacgcg cgaaatgcga aaaaagggct cgaacaccag
     7021 cttgcggtat tcttcgggaa ttccctgcac gtaatcggag tctgtgcgca gcggctcgtt
     7081 gagcaccgtg acaagcaccc gggtaaactt gtactctatg agcaccgtga cagtcgattc
     7141 gtcttccgaa aattttaaag cattgatcag aagttcgcgc agtgccttgc gcatgaactc
     7201 ccagttcatc ttgaggactt ccccggaaaa gcgcggcggc aattcactga gcacgacggc
     7261 atggcgccgc atttcaataa cggggttaag ttcaaatatc aggtcgctga caattttatg
     7321 gaattcaccg agtgatacat cgtgcagcac caggtcttgc gagacgagca ggtgcagctc
     7381 tgagaacatc tcgatcatcg cgttcactgc gtcctggttg tcgttgatga gatcgagtat
     7441 atcttttgga accaggtaat tctgtttatc ttcggtgagc tgcgctccgc cgagcatcga
     7501 gaccagggtc gtcaacgacc cgacaccgcg gccctgcgaa aatgaagtga acatgttgcc
     7561 gaacaacgcg cgcgcaaact tgtcattctg ccgccgcacc agctggtcag ccacctggcg
     7621 gctggctaac cgtttttcat aatattcggc gcgctcacgc tcagcgatgc gcgccacaac
     7681 ttctgactgc gaccgggcaa aggcacgacc tatgacgtca tcgagttcgg cgctctgcac
     7741 gggcttcacc aggtagtcgt aagcacccag tcgcatcgtt tcaataacgt tgtcgatggc
     7801 cgaatcgccg gtcagcatca cgacgacggg ggggtgcgga gtttctttga gacgcctcag
     7861 gacatcgacg ccagagagtt cgcccggaag gttaatgtcg cacagcacga cctcgtggcc
     7921 gactttttgg aatgcatcga gggcttcctg tgcagtggca accaccgtaa gcgaatacgg
     7981 cataaaagag agcatgcgct cgataagcgc ctgttgcacg ggatcatctt caaccaggag
     8041 caggggtttt ttcttcagca tagccaggcg ctgtgcttaa tgtcggcaga ttggggggcc
     8101 attctgtatc agggagcgca gaatgtagca cgcatgagct cccccatgcg aaggagccac
     8161 taacagcgat cgttaaacga tatggatgtc gacgcgcttg ccgctgacgc tgccggcgtt
     8221 ctgcagctcg atctcgtact tgccgactgc aagttgcgaa aaccaggcaa agtcgtcgcg
     8281 caggggcatc gattgcagca atcggccctg gcggcgcagg ttgataaatt tcggccgcgg
     8341 gcggtaattc tgcagcttca ctgtcagcat cacggtatcg gcaccatctt tgaccacctg
     8401 gtaaacgagg tcaccctggt tggcttcgct cgtcatgaac tgcagggcgc cagtcgggtt
     8461 gccggcatgg gctgaagcct ggtcagaacc tgagcgcacg gccaccatgt cgtcggggtt
     8521 ggtaatgtta tacaagtcgc ggatgtgccc ggcaataagg cgcaggccgt ctcgcaggcg
     8581 aatgacgatc gtcgactcac catcttgcgg ggcctcggcg tagatacggt tcatgactat
     8641 ctgcttgagg tatgccgggg tcggcagcat cggccgttcg ccccaggtaa aaaggaatgc
     8701 aagggtatcc tggaagaggg caaaatcggc ggggctggcc ttaatcgcat tccaaatgcg
     8761 gcttgcctgc agatcttttt ccatcgcgcg cgggtcttca acgctgaaga atttttcagc
     8821 gattgcaacc acctcgtgca gcgctacctg gaaactggca ggttgcgtgg tcgcctggtg
     8881 tcgcttgctg accgcaacca tcgtctcaaa ttgcaccagc atctcgagcg ccgaaaggtt
     8941 ctctgaggcc tgcttgatcg cctgcggctg tttatcctca tcaaggtaca gcttcaaaag
     9001 gctggaatac tccgccggtg tcagtttctc cagattctta cgttgttttt ttgtatccac
     9061 aagtaccacc cggcaggtgc acaagcgccc ctgctttgct tctgttatag acgacggcgg
     9121 ggggttacac tttacgacaa gaaaaagaag gatccatgct gaggctcatt accatctcca
     9181 tcagccacta tgtcgaaaag gtcaaatggg cgcttgagtt gagcggtatt gattataaag
     9241 aagaaagcca tattcccggt ctgcacgctg cggtaacgct ctggcatacg cggggccggc
     9301 accgctcaac tcccgtgctg atagacggca accaggtggt acctgactcg accgcgattc
     9361 tgcggcacct cgccgataaa tatgaccagc gctggttgta ccctgcccct aatgctctgg
     9421 agctcgaaga acgtttcgat acagcggtgg gacct
//
LOCUS       DAOQJF010000096         9426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3861_length_9426_cov_4.850496, whole genome shotgun sequence.
ACCESSION   DAOQJF010000096 DAOQJF010000000
VERSION     DAOQJF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3861_length_9426_cov_4.850496"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..760
                     /locus_tag="PLF85_06795"
     CDS             116..760
                     /locus_tag="PLF85_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19406.1"
                     /translation="MTNWQTMKKSFLTGVFFTLGIVTTAIVAVTVSATFTSGDTLTAA
                     NMNVLKTAIESIPDWTKSGSDAVFTGGNVQIGNGSTSRDLAVYGKVSSTALGTFCGVT
                     ASTYDGTGVGGWAGGKTKCEVACGDTRAHMCTEHEFQISAQLGIDFSPMASSDHWYAA
                     NMSEYTAPSQQTSNCGGWTSNNSAAYSRTVTQISGRPSSGVTACNAARRISCCR"
     gene            770..1351
                     /locus_tag="PLF85_06800"
     CDS             770..1351
                     /locus_tag="PLF85_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19407.1"
                     /translation="MMDITLPLTVLEKTAGAAKTLSRSSDKTNALVNLLKIEMQTNLG
                     LLNKALEKEKTTKHGNAKIGETHNDPEPELSEPAFVDLLLQVVSFGNLYIALTCTDEA
                     SNLRRQLQKETNTSKKDGTSFTYENLGIRILRKFQLLKALARFQTERPRLVQKFMTAT
                     RYHRRLNSLKNDVEQMASFVNTLSPVKWYQCLW"
     gene            1768..2199
                     /locus_tag="PLF85_06805"
     CDS             1768..2199
                     /locus_tag="PLF85_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE19408.1"
                     /translation="MLQNDYSPLLFKEGWRKAPGWFAPINLLTTRFYESAYAMQLPSG
                     INIPENTVAEICEKWGISKLELFGSALTANFKPDSDIDLLATFDSARPLGFRVFDAVD
                     DFSRALNRKVDLVSRESLLRSQNKFRLRRILASARPIYERS"
     gene            2186..2527
                     /locus_tag="PLF85_06810"
     CDS             2186..2527
                     /locus_tag="PLF85_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNE19409.1"
                     /translation="MNDRDIGYAIDILIACRQIEQFGADRPADSLSRDAMYQAAVLRK
                     LEIIGEVAKRLSNEFKSAHAHINWKGWAGLRDRLIHGYDDVNLDIVQTVLSDELPRLM
                     AALKPYESNNR"
     gene            2779..4323
                     /locus_tag="PLF85_06815"
     CDS             2779..4323
                     /locus_tag="PLF85_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNE19410.1"
                     /translation="MAKIFVDGAEYEVDEKKNLIDALKEKGVEIPHFCYHSKLSVVGM
                     CRICLIEVEGVPKFQAACNTPVKDGMKINAFGEKIVKAREGVMEFLLINHPLDCPVCD
                     KAGECRLQNYSFSLGNETTRYKEVKRNIPQEKIGTNLLINHNRCILCYRCVRFDREVV
                     GVNDLEMLARGNDTIIAYTPPETSGEKSTYLNHNYQGALADICPVGALLNENTLFQSR
                     VWWYESKESHCHGCSTLCKVTTNVKNNALYRYMPPQNQATDGYFICDYGRFSGKDFSQ
                     DRLHHYALNGEKSRSTEALPRLAEKAAAAKKILVLGGATESCEDVDALEAQINTWRAQ
                     GKEVLWDFRTDKPAFHDGKSQWDFLLSRDLRPNAKYLRDKSATQLADYAALEKELRAS
                     DLVIVVGELAAPYAYQTGEKHANVQGLTIAEKVEDTDLFQSLNSALAWGKTALFTTHL
                     NAATELAAIAMPVLAFPEKAASYIDKQGQKKVTTAVLQHVQGLQSPAGVLARIKELTT
                     TAGAAV"
     gene            4323..4841
                     /locus_tag="PLF85_06820"
     CDS             4323..4841
                     /locus_tag="PLF85_06820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNE19411.1"
                     /translation="MATIQVSKKYAFKWYEKFYFMSVSAGMWVTLKHFIKSAVLRRMV
                     TVQYPEQKKPLSSRFRGLHRLKRNEKGQERCTACYCCQWVCPADAIQIVAAEVTPERQ
                     YLHPEDKYAKTFQVDLLRCIYCGLCEEACPKGAIYLDGGMPMPADSRDGLILDKEKML
                     ETNGGPILGERV"
     gene            4838..5893
                     /locus_tag="PLF85_06825"
     CDS             4838..5893
                     /locus_tag="PLF85_06825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE19412.1"
                     /translation="MSFSNRIKRKTAEVRKRIREKGNERITFLVIPHNDKAIVNMQLS
                     NYVIALGIFSLIFIVVASVFAVNMQRNIQGEADKLQDRDKAYVNERELYVRKYHEMVV
                     QQDLFKKQFGKVIKQARLLEGEEEIFIDDDILRDSAVQMLDAEGEDYARSLSYKENRT
                     IKFLNFETTLKMAQGKSQEEGFYFYPEVTLYRKLKLDMKQTTGAVVMLQQLLKEREVV
                     QQALPYHWPLAGGHFTSFFGGRISPFGHSRDFHTGVDLADATGTPIYAAADGRVIGCG
                     YSGGYGLSAKISHPYGFQTLYGHMSSLYATCGSYVRKGQMIGRVGATGRATGPHLHYE
                     VRIMDHPVNPMPYLTSG"
     gene            5924..6715
                     /locus_tag="PLF85_06830"
     CDS             5924..6715
                     /locus_tag="PLF85_06830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE19413.1"
                     /translation="MKISACIIGLNEEQNITDCLASLQGVADEIVYVDSYSSDKTVAL
                     VKKFTKRVFYRKFDNYVAQKNFAAAKAKHDWILNLDCDERLSDELRASLLALKATSTD
                     KAGFRFNRLTWYLYRFIRHSGWYPDDKIRLYDRRVAQWEGEKVHEIINTPAEKTGKLK
                     GDLLHYSFRTVDDHLKTIRNYSEMAAQAMYARGRRVSLAGVFARSGWVIVRKFFFELA
                     FLDGTAGIIITYYSAVATFTKYIKLYVLGKQGRTAHLNRPQGRFK"
     gene            6712..7314
                     /locus_tag="PLF85_06835"
     CDS             6712..7314
                     /locus_tag="PLF85_06835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE19414.1"
                     /translation="MSNQSVWDNHYTRDRSRQQFPDENVVRLLRKNFAGERRTLALDL
                     GTGSGRHLPLLVEHFENVLACDFSRESLRIAAGDKIQCAQAALPALPFASGTFDFILC
                     WGVLHYLEESALAPAIAAIHEILKPGAGLFLTLRSDQDSHLALQLQRGDLAGGHARLY
                     SKEEALSLFGRFAKVDYGFIARQPLGEEGLVAHHMLFARR"
     gene            7311..8222
                     /locus_tag="PLF85_06840"
     CDS             7311..8222
                     /locus_tag="PLF85_06840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE19415.1"
                     /translation="MKKKALSCPVCASVKVVQKYLIERHNFSVHACADCGSEFQYPLP
                     KNADAFYDEGYYTGSANFSYQDERKAERFHNYVHFARLRTIQSAVGKKHLKLLDVGCA
                     FGAFARAAAHFGEAYGLDVSQFAVQEGNKISQAMNSQARLFEGDLTHLPKTADAKHIF
                     AAGSFSAITLIEVAEHLAEPRPALAAAYDLLAQGGVLVIQTANFEGLQARDAGSGYHY
                     YLPGHLTYFTATGLRRLLSELGFREFKEFFPTDFPLWAKWRKAWGDVKRPGDLLRFWR
                     MSVYHIKSKFKRGGRPLTSSYVLYALK"
     gene            complement(8249..9346)
                     /gene="mraY"
                     /locus_tag="PLF85_06845"
     CDS             complement(8249..9346)
                     /gene="mraY"
                     /locus_tag="PLF85_06845"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNE19416.1"
                     /translation="MLQLLLTPLQKHVSWFRIFNYTSFRLVAAAITALLLAWIFGPRF
                     IRFLRKLKFGESVRSDGPQSHQSKAGTPTMGGLLIMASMSVSILLWGNLSNLYVILIW
                     LGTLALSMLGFIDDYSKSVLKISGGMRARTKFFWQIVIALSFAVLVYMFPYTAKGAPE
                     QATYLYLPFIKSPVLNLGGAAIFFWMLVVVGTSNAVNLTDGLDGLAAGISVIVIGTLA
                     IIAYITGLEEASRYLLLVHVPEANEVSVFLAALGGACIGFLWYNAHPAEVFMGDTGSL
                     AIGGAVGMAAILIHREILLMILGGIFVAEALSVIFQVGSYKLRQKRIFRMAPLHHHFE
                     LGGWHENKVVIRFWIVGVLLALVSVSSLKIL"
BASE COUNT         2359 a         2620 c         2495 g         1952 t
ORIGIN      
        1 ctcgggcacg gtgaatgcag cgaatgtgac gggtgtgggg gtgagttgtg cgagtgtgac
       61 gtgtgtgctt aataccagta ccttagataa ctgcaaactg caataggaga aacaaatgac
      121 taactggcaa actatgaaaa aatcctttct gaccggcgta tttttcacgc tcggcatagt
      181 aacaacggca atcgtagctg taaccgtatc agcaaccttc acttcgggcg atacccttac
      241 cgctgcgaac atgaatgtgc tcaagacggc gatcgagagc atacccgact ggaccaagtc
      301 gggcagtgat gcggtgttta cggggggtaa tgtgcagatt gggaacggca gcacgagcag
      361 agatttggct gtttatggta aggtaagctc tacagctctt ggtactttct gcggtgtgac
      421 tgccagcacc tatgatggca cgggggttgg gggctgggcc ggtggcaaaa cgaaatgcga
      481 ggttgcctgc ggcgacacaa gggctcacat gtgtacagaa catgaatttc aaatcagtgc
      541 ccagctaggc attgatttta gtcctatggc ttcttcagat cactggtacg cggcaaatat
      601 gagtgaatac acagcgccta gccaacaaac tagcaactgc ggtggctgga cgagcaacaa
      661 cagcgcagcc tatagcagga cagtgacgca gatatcgggc cgtccaagca gtggcgtgac
      721 cgcctgcaac gctgctcgaa ggatttcttg ctgccgttga gtaaaagcta tgatggacat
      781 cacattacct ctgacggttc tggaaaagac ggcgggagca gcgaagacgc ttagccgcag
      841 cagcgacaaa acaaatgcct tagtgaatct gctaaagatt gaaatgcaga caaatttagg
      901 cctcttaaac aaggcacttg agaaagaaaa gaccaccaaa catggtaacg ccaaaattgg
      961 agaaacgcac aatgatcctg agcctgagct ttctgaacct gcatttgtcg atctgcttct
     1021 tcaggtagtt agcttcggca atctctatat tgcgctcact tgtaccgatg aggcttcaaa
     1081 cctgcgacgc caattgcaaa aagaaaccaa taccagcaaa aaagatggca catcattcac
     1141 ttatgagaac ctcggaattc gtattctgcg gaaatttcaa ctgctcaaag cacttgctcg
     1201 ttttcaaacg gagagaccaa gactcgtgca gaagttcatg accgctacgc gatatcatcg
     1261 acgacttaat tcactgaaga atgatgttga gcaaatggcc agcttcgtga acactctatc
     1321 accagtgaaa tggtatcaat gtctttggtg aaaaatcgtt aatgtctttt cgcgagtaaa
     1381 gtgcagagca cctgccctcg tttccggacc acttgacacg cacctacccc tgcttcacag
     1441 ggggacaaaa ccaccccgcc cctacggggc acccctccga aggaggggaa ttagtgggac
     1501 agactcgccg ccactgggaa cttgcagaac catattcccc tccttcggag gggtggcagg
     1561 cgtcccgccc tgagcgtagt cgaagggagc ctgacggggt ggttcctccc tcggtacaat
     1621 gcccattatg gcactcacac ccgcaactca cacgcaatct gactcaactc gcctgcgagt
     1681 gtgacacgca aaggcagtca aagaaccccc tcccggcttc gccgtactcc cccttggcag
     1741 ggggagagag tatgtgttct gtgcacattg cttcagaatg attattctcc cctccttttc
     1801 aaggaggggt ggcgcaaagc gccggggtgg tttgccccca taaacctctt gacgacgcgg
     1861 ttttatgaat cagcatacgc catgcagttg ccttcaggca tcaatatacc tgagaatacg
     1921 gtggcagaga tctgcgaaaa atggggtatc agcaagcttg aactctttgg gtcggcgttg
     1981 accgcgaatt ttaagcctga cagcgatatc gatttgctcg caacttttga cagtgcacgt
     2041 cctctcggtt tcagggtatt tgatgcagtg gatgacttca gtcgcgctct caatcgaaag
     2101 gtcgatctgg tatcgcgaga aagtctgctg cgaagccaga acaaattcag gctcagacgc
     2161 attctagcct cagcgcgccc gatttatgaa cgatcgtgac atcggttatg ccattgatat
     2221 tctgatcgcc tgccgccaga tcgagcagtt cggcgcagac agaccggctg actccctttc
     2281 aagggatgca atgtaccagg cagcggtatt gcgcaaactc gagattattg gtgaggtcgc
     2341 caagcggctg agcaacgaat tcaaatctgc gcatgctcac atcaactgga aaggttgggc
     2401 tggtctacgc gacagactca ttcacgggta tgacgatgta aatctggata ttgtgcagac
     2461 tgtgctctca gatgagctcc cacgactgat ggcagctttg aagccttacg aatcgaataa
     2521 tcgctaaacg gtacgggaca aaaccacccc gcccctacgg ggcacccctc cgaaggaggg
     2581 gaattagtgg gacagactcg ccgccactga cgactttcag aaccatattc ccctcctccg
     2641 gaggggtggc aggcgaagcc tgacggggtg gtttcctacc actcgcaaag cgagtgacgg
     2701 ggtggttgcc cctataaacg agggaaatcc tctcgttcaa ataaatcgac accctgattt
     2761 cgcttgcaga atgtgcccat ggctaaaatc ttcgtcgacg gggctgaata cgaggtcgac
     2821 gagaagaaaa acctgatcga tgcgctcaaa gaaaagggcg ttgagatacc ccacttctgc
     2881 taccactcga aactttcggt ggtgggcatg tgccgcatct gccttatcga agtagaaggt
     2941 gtgcccaagt tccaggctgc ctgcaatacc cccgtcaaag acggcatgaa gatcaatgcc
     3001 ttcggcgaaa agatcgtcaa ggcacgcgaa ggagtgatgg agtttctgct catcaaccat
     3061 ccactcgact gccccgtctg cgacaaggcc ggtgaatgcc gcctgcaaaa ctattcgttc
     3121 tcgctcggca atgaaaccac gcgctacaaa gaagtgaagc gcaatatccc gcaggaaaaa
     3181 atcggtacca acctgctcat caaccataac cgctgcatcc tctgctaccg ctgtgtgcgc
     3241 tttgaccgcg aggtcgtcgg cgtgaacgat ctcgagatgc tggcgcgcgg caacgacacc
     3301 attatcgctt atactccgcc tgaaaccagc ggcgaaaaat cgacctacct caaccacaac
     3361 taccagggag cgctcgccga catctgcccg gtgggtgcct tactgaacga gaacaccctt
     3421 ttccagtcgc gcgtctggtg gtatgaatcg aaagagtcac actgccacgg ctgctcgacg
     3481 ctgtgcaagg tgacgaccaa cgtcaaaaac aacgcgctct accgctacat gccgccgcag
     3541 aaccaggcta cagacggcta cttcatctgc gactacggcc gattctcagg caaagatttt
     3601 tcgcaggacc gcctgcacca ctacgctctc aacggcgaaa agtccaggtc gacagaagct
     3661 ttgccccggc tggctgaaaa agccgccgcc gccaaaaaga ttctcgtgct cggtggcgcg
     3721 accgaaagct gcgaagacgt cgacgcactc gaagcgcaga tcaatacctg gcgcgcgcag
     3781 ggcaaagagg tgttgtggga tttcagaact gacaagcctg cattccacga cggcaagagc
     3841 cagtgggatt tcctgctgag ccgcgacctg cgccctaatg caaaatacct gcgtgacaag
     3901 agtgcgaccc agctcgcaga ttatgcagcg ctcgaaaaag aactccgcgc cagcgacctg
     3961 gttattgtcg tcggtgaact ggcagcaccg tacgcctacc agacaggcga aaaacatgca
     4021 aacgtgcagg ggctcaccat cgccgaaaag gtcgaagata ctgacctctt ccagtcgctc
     4081 aactcggcgc tcgcgtgggg caagactgcg ctgtttacta cccacctgaa tgccgcaaca
     4141 gaactcgctg ccatcgccat gccggtactc gcgtttccgg aaaaagccgc cagctacatc
     4201 gacaaacagg gacagaaaaa ggtgacaact gccgtgttgc agcacgtcca gggtctgcag
     4261 tcgcctgcag gcgtattggc gcgcatcaaa gaactcacca ctaccgcagg agcagccgtc
     4321 taatggcaac tatccaggtt tcaaaaaagt acgcattcaa gtggtacgaa aagttctact
     4381 tcatgtcggt ttcagccggc atgtgggtga ccctcaagca cttcatcaag tcggcagtgc
     4441 tgcgccgcat ggtcaccgtg cagtatcccg agcagaaaaa gccgttgtct tcgcgctttc
     4501 gtggcctgca ccgtctcaag cgcaacgaaa aaggccagga gcgctgtacg gcctgctact
     4561 gctgccagtg ggtctgccca gcagatgcga tacagattgt tgccgcagaa gtaactcctg
     4621 agcggcagta ccttcacccc gaagacaagt atgcaaagac attccaggtt gacctgctgc
     4681 gctgtatcta ctgcggcctg tgtgaagagg cctgtcccaa gggcgcaatc taccttgacg
     4741 gcggtatgcc catgccggca gacagccgcg atggcctgat tctcgacaaa gagaagatgc
     4801 tagaaaccaa tggcgggcca attctcggag agcgtgtttg agtttttcca accgcatcaa
     4861 acgtaaaacc gccgaagtcc gtaaacgcat acgcgaaaag ggcaacgagc gtatcacatt
     4921 tcttgtcatt ccgcacaatg acaaggcgat cgtcaacatg cagctctcga actatgttat
     4981 cgcactcggc attttttcgc tgatctttat tgtcgtcgct tcggtttttg cggtcaatat
     5041 gcagcgcaat atacagggcg aagccgataa actgcaggac cgcgacaaag cgtacgtcaa
     5101 cgaacgcgag ctctatgtgc gcaaatacca cgagatggtg gtgcagcagg atctcttcaa
     5161 aaagcagttc ggcaaggtga tcaaacaggc caggttactc gaaggcgaag aagagatctt
     5221 catcgatgac gacatcctgc gcgacagcgc cgtgcagatg ctcgacgccg agggtgaaga
     5281 ctacgcgcgc tcgctcagct acaaagaaaa ccgtacgatc aaattcctta acttcgaaac
     5341 caccctcaaa atggcgcagg gcaaatcaca ggaagaaggt ttctatttct accctgaagt
     5401 tactctctac cgcaaactca agctcgacat gaaacagacg accggcgccg tcgtcatgct
     5461 gcagcaactg ctgaaagagc gcgaagttgt tcagcaggca ttgccctacc actggccgct
     5521 cgcgggcggt cacttcacgt cgtttttcgg cgggcgcatt tcgccgttcg gccattcgcg
     5581 tgacttccat accggcgtcg atctcgctga cgccaccgga acccccatct atgctgcggc
     5641 agatggcagg gtcatcggct gcggctatag cggaggctac ggcctgtctg ccaagatctc
     5701 gcatccatac ggcttccaga ccctctacgg ccacatgagc agcctgtacg ccacctgcgg
     5761 cagctatgtc cggaaaggcc agatgattgg ccgggtcggc gcgacgggcc gcgccacggg
     5821 cccccacctg cactacgaag tgcgcatcat ggatcacccg gttaacccga tgccttatct
     5881 aaccagcggc tagtaccgct gaataggcaa cgcctgaaac cccatgaaga tctcagcgtg
     5941 catcatcggc ctgaacgaag agcagaatat tactgactgc ctggcatcgc tgcagggtgt
     6001 cgcggacgaa attgtctacg tcgacagcta ttcgagcgac aagaccgttg cgctcgtgaa
     6061 gaagttcacg aagcgtgtct tttaccgcaa gttcgacaac tatgtcgcgc agaagaattt
     6121 tgctgcggca aaggcaaagc acgactggat tctcaacctg gactgcgacg aaaggttaag
     6181 cgacgaactg cgtgcctcgc tgctggcgct caaagcaacc agtaccgaca aggccggttt
     6241 tcgctttaat cgcctgacct ggtacctcta ccgcttcatt cgccactcgg gctggtaccc
     6301 tgacgacaag atacggctct atgaccgccg cgttgctcag tgggaaggcg aaaaagtaca
     6361 tgagatcatc aacaccccgg cagaaaagac cggtaagctc aagggcgacc tgctgcacta
     6421 ttctttccgt acggttgacg accacctcaa gaccatacgc aactattctg aaatggcagc
     6481 gcaggcgatg tacgcgcgcg gccgcagagt ttcgctcgcg ggagtttttg cacgttctgg
     6541 ctgggtgatc gtgcggaagt tcttcttcga acttgccttt ctcgacggca ccgctggaat
     6601 cattatcacc tactactctg cagtggcgac ttttacgaag tatatcaagc tctacgtgct
     6661 gggcaaacag ggccgaacag ctcatttgaa ccggccgcaa ggccggttca aatgagtaac
     6721 cagtctgttt gggacaacca ctacacccgc gaccgctcgc ggcagcagtt tcccgacgag
     6781 aatgttgtac gcctgctgcg caaaaatttt gccggcgaac gtcgtacgct tgcgttggat
     6841 ctgggtactg gtagcgggcg ccatttgcca ctgctcgtcg aacattttga aaatgttttg
     6901 gcctgcgatt tttcacgcga aagcctgcgc attgcagcgg gcgacaagat acaatgtgca
     6961 caggcggctc tacccgcgct tccgttcgca tccgggacat ttgacttcat tctttgctgg
     7021 ggggtgctgc actatttaga ggaaagtgca cttgcaccgg ccattgccgc gattcacgaa
     7081 atccttaaac ccggcgccgg tcttttcctc acgctgcgct ccgaccagga tagtcacctc
     7141 gcgctgcaac tgcagcgggg cgacctggct ggtggacacg caagactcta ctcaaaagaa
     7201 gaggcgctgt cactctttgg ccggtttgcg aaggtcgact acggttttat tgcacggcag
     7261 ccactgggcg aagagggcct cgtcgcccac cacatgctgt tcgcgcgccg atgaaaaaga
     7321 aagcactgtc ctgccctgtc tgcgcttctg ttaaagtcgt gcagaagtac cttatagagc
     7381 ggcacaactt ctcggtccat gcctgcgccg actgcggctc tgaattccag tacccgttac
     7441 cgaagaatgc tgatgctttt tatgatgaag gttactacac gggtagcgca aacttcagct
     7501 accaggacga acgcaaggcg gagcgattcc ataactacgt acatttcgcg cgcctgagaa
     7561 ctatccagtc agccgtggga aaaaaacacc tgaaactgct cgatgtcggt tgtgcctttg
     7621 gcgcatttgc ccgtgccgca gcgcacttcg gcgaggcata tggtctggat gtctcccaat
     7681 ttgccgtaca agagggaaac aagatctcgc aggcgatgaa ttcccaggca cggctttttg
     7741 aaggcgacct cacgcacctg cccaaaacgg ccgacgcaaa acatatcttc gctgcgggta
     7801 gcttttctgc aatcaccctt atcgaagtcg cggagcacct ggccgaaccg cgccccgcac
     7861 tggctgctgc gtacgacctg ctggcacaag gtggcgtgct tgtgatacag acggcaaact
     7921 tcgaaggttt gcaggcccgt gatgccggtt ctggctacca ctactacctg ccgggacacc
     7981 tcacctactt caccgcgacg ggcctcaggc gtctgctcag cgagctgggg tttcgcgaat
     8041 tcaaagaatt tttcccgaca gactttccgt tatgggcaaa gtggcgcaag gcatggggcg
     8101 atgtaaagcg gccgggggat ttactgcggt tctggcgaat gtccgtatat catatcaaat
     8161 cgaaattcaa acgcggcggt cgcccgctga caagcagcta cgtactctat gcattgaaat
     8221 aatgccgcaa cgcggcgtca tttcaatgtt aaagaatttt caaactcgaa actgaaacca
     8281 gggcgagcag aactcccaca atccagaagc ggataaccac tttgttctcg tgccagccac
     8341 cgagctcgaa gtggtggtgc agaggcgcca tgcggaagat acgtttttgc ctgagtttgt
     8401 aagaaccaac ctggaaaatg acgctcagcg cctcggcgac gaaaatgccg cccaggatca
     8461 tcagcagaat ttcgcggtga atcagaatcg cagccatgcc gaccgcaccg ccaatcgcga
     8521 gcgaaccggt gtcgcccatg aagacctctg ccgggtgtgc gttgtaccac aagaaaccaa
     8581 tgcaggcacc acccagtgcc gcaagaaata ccgatacttc gtttgcttcg ggtacatgta
     8641 ccagcaacag gtaacgcgat gcttcttcaa ggccagtaat gtacgcaatg atcgccaggg
     8701 tgccgatcac gataactgaa attcccgccg cgagaccatc gagaccgtcg gtcaggttaa
     8761 ctgcattgct ggtacccacg accaccagca tccagaaaaa tatggcagcg ccgccaagat
     8821 tgagaacagg gcttttaata aaaggcagat aaaggtacgt ggcctgctca ggtgccccct
     8881 tcgccgtgta aggaaacatg tagacgagta cggcaaatga gagcgcaata acaatctgcc
     8941 agaaaaactt ggtacgcgcc cgcataccac cgctgatttt cagcaccgat ttgctgtagt
     9001 catcgataaa accgagcatg ctgagcgcga gagtgcccag ccagatcaga ataacgtaga
     9061 gattactgag gttaccccag agcagaatcg agacgctcat actcgccatg atcaaaagcc
     9121 cgcccatagt gggagttccc gccttgcttt ggtgcgactg cggaccgtca gagcggacag
     9181 attcgccgaa tttcagcttg cgcagaaagc gtatgaacct gggcccgaag atccacgcga
     9241 gcaacagcgc ggtaattgcc gcagcgacca gcctaaacga cgtgtagttg aagatacgga
     9301 accacgaaac atgcttttgc agcggtgtca gcaggagttg cagcattaag gagctttttt
     9361 accggacggg cacaggttga aaatgacttt tgggcaccga aaaaaatgcg atcaagccct
     9421 ctacgg
//
LOCUS       DAOQJF010000097         9412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3867_length_9412_cov_4.894304, whole genome shotgun sequence.
ACCESSION   DAOQJF010000097 DAOQJF010000000
VERSION     DAOQJF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3867_length_9412_cov_4.894304"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLF85_06850"
     CDS             <1..765
                     /locus_tag="PLF85_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE19417.1"
                     /translation="ADRRVSEVETDSPVGMQQVTKPRLGQQVQGVELIHAQAADFFHD
                     FNPVLSGISFSIRTGQRVLVTGSNGSGKTTLLRVLHGDFYPAYGCGDLQFRGELAHEQ
                     KRDLWKKVQFIAAAHFDYFLPGMSVEDVLASRLSGSLYDYDPVLPDAALQVAEQFGLL
                     QFLPRPFARLSEGEKTRVLLCRAFLAPAPVYLIDEGFMALSGRYFELVTQYLNALPAE
                     AVTVIAANERISALQARLEFPLLHWQMERGRLTILP"
     gene            complement(686..2389)
                     /locus_tag="PLF85_06855"
     CDS             complement(686..2389)
                     /locus_tag="PLF85_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE19418.1"
                     /translation="MKMHNILYTGAFQKLRAHLTKSGKLPLPDLVEIVRDELDLSMML
                     SLRDRERIPAQGRLLIVANHPLAFLDLVALYTVVSDIRKDVVAVVEPLSEFAATDSHV
                     ILAQKGAERTTVRAIHRALASEKAVIVFPAKSRHWGLSLVVDGDWSKAVVQMAQHWQV
                     PVLPVHIGARNRLTHYFWTAVGGMYSDIALDAEIKSQLHRSIAIRLSHPIMPESLAGV
                     KAGAAAKLLRRHVYQIGKGKPGTFRTTKNIVHPTDKKLILNDVMAAELLGQTEDKIKI
                     LLVRYEAAPALMREIGRLRELTFRKVGEGTGRKIDLDEFDQYYQHIVLWDESQLEVVG
                     AYRIAAGDEIYRRLGISGFYTSTLFRFSEEFEKYLPQSLELGRSFVQPNYWNSQALDY
                     LWHGIGAYMAARPQYNYLFGPVSVSNSYSPEARELLVHFYQRWFGKKDAPVRAKHRFR
                     FSQGAEDRLARAFSGNEYKQSFKELKQNLKHYGFAVPTLYKQYTELCEEGGVHFLDFG
                     IDKDFSNALDGFIFLSIAHVKESKLERYVYSKRSKEEWSAARAPFASGVREIPVVPAA
                     R"
     gene            complement(2459..3469)
                     /locus_tag="PLF85_06860"
     CDS             complement(2459..3469)
                     /locus_tag="PLF85_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNE19419.1"
                     /translation="MGTTKEILRFDRWERRLVRLVRRSDGMVRIRQLDFSSPTRDGQR
                     YPIYLLEIGKSSAFKRHAVSIVSGVHGLETIGIRIHLDMIKCFLNPKSSHFSQALAEG
                     KFGIYSLPILNPAGVARETRANGRGVDLNRNSGIDAEGALPFFGGHRLSPALPYYRGR
                     ALERESRALYRFLREYAWKSGRLHTALDIHSGYGAQNYLWWPYSYSKRRVPHASVFQH
                     MAAELADRHPLYRIEPMSLSYQTHGDLWDRALLEFEMSKQKGSIARKNLFLPLTLEIG
                     TWQELKKNPFRMVQKQNIFNPPPQSRKTYLREHRQLICDLLHLHVKKSDQRRYTRLRD
                     AS"
     gene            3582..5789
                     /locus_tag="PLF85_06865"
     CDS             3582..5789
                     /locus_tag="PLF85_06865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19420.1"
                     /translation="MRRLFLPLFYFVANVISYGAAAASSALYGGIVTFVFVLYYSYAI
                     KLPAIDELILVVGGVAAFTTIIHGVYFGVLHKIGISGITRGMRLLNRAIVRGKTWFEV
                     DIRQNLNADEYRELHRMLCYLPRENAYMSVTMVLMIVGGVLLYLYRWQNYSFINMVQV
                     VVIAVIAAFVHAGFTAVITELVTGEMRTRVKQIMYEKRVEFQEIALSNVRVKILLFIM
                     IMIATLFVSNFMVYYNVEFPVLIRFSVFAIFIGAIMAYMLFSIVLQSLHEIEVASVSL
                     RDGKDAQLFPQALDSEFINVATGLNTAVHTIRDYQHNLERKVDERTAELTAANESLHA
                     KDRLIQMELDFAAEIQKGIIPAQIEEWNGLRFYGYYKPMEKVSGDYYDVFPTQGNKLG
                     ILMADVSGHGVPAALITTMAKVAFARSASTTPSPAKTFEEVNNQLLGLVTTQDYLTAF
                     YLTIDETHSFLYGNASHQQAKIVRNKNIESLDTGGLFVGAMHEANESYEEKEGRLYAG
                     DRMFLYTDGLIEIRNLDDEEFGIERFDALLIAASDLPPEQAVSFIVEEVFRFAAGRKP
                     NDDISILMIDVNREYSRFLQIASRAYQVIEQGDRQEGVRLLDEAIRLYGRNPLSLRTA
                     GEVNFDLGRIDTAERYFKAYLDLTRQNAEVFCYLSSIAILRTRYAEAEKFAREAVALR
                     SNYALAYNNLAIACLNQGKNALARFAMEKAITYEPENAEFQKNAAQLERVLGE"
     gene            5835..7337
                     /locus_tag="PLF85_06870"
     CDS             5835..7337
                     /locus_tag="PLF85_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNE19421.1"
                     /translation="MRKVLFLSAFFVAVFCSESLFAARKFALIVGTNYKGSEISPLAL
                     CEKDAEVMKQSIVKTGNFDAANVRVLLGNQVTRENLRSSIVEWLGRNVKEGDQVFFFF
                     AGHGQFMRDPAAKNGMRNYVIMFQRPHVSDDELSEWFGKLRTKKAVIILDCCFSGGIA
                     KKGAATRGAGNVPIPEGQDYAVLQDLENVHFQNKVVISSADDNETAIEIGKPIEHGIF
                     TYFFSKAILEADLNGDKSITAYEAFYKARNETTKLAAKANHKQVPQISGDASGFMFVA
                     APTPTTPPQPVLPPVPVTVPEPVIAPPITPAEPQNPQNTKTGTLVIKTTYTQAQLSGG
                     LQVFVGQDEYDFKLSYVEFGAWGKTAQITLSKIPSGVHNVTIKAGGYADQVIKTGIEP
                     NGTTVETVTCGLAGKGAIAGKVYLETFDQPAEGFIVFIKPLPNFKQPTSTSRKDGSFA
                     FTEVKPGKYTVFLRGSGRALIKQYDQEITVEPDRITNLDIILRNIFNKKK"
     gene            complement(7392..7895)
                     /locus_tag="PLF85_06875"
     CDS             complement(7392..7895)
                     /locus_tag="PLF85_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19422.1"
                     /translation="MRSVLFVTIAGLFMACNGRGRFAFTVADHTNLPVLEQRFFHPQR
                     FVTYQQTPVFERDDVLWYAYRPASPQSGAYYGISLQKKSLGYQEIDLRNRQIAEGQGM
                     LIDHYRNLEEGEYRLKIAYNNRVIDQIDFVVVADSASESVNFEEDETDTGEGMPMASA
                     QSPSTDF"
     gene            complement(7946..8887)
                     /locus_tag="PLF85_06880"
     CDS             complement(7946..8887)
                     /locus_tag="PLF85_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19423.1"
                     /translation="MTKWIIPVLLLVLAQCRKNPHISASEIAELAKVKPEKVLVQKKY
                     QLDSSASFEDITLFEAQGKTHLVIFTPADKGYALLRLHAFAGLLNEPKLFFIAVGNKK
                     SQVLVIHGTDKKTLHLFDGGSFVQEFTPDTADTATVKMTKVEEKPNDELLNLGAVNYR
                     FNGMRWVPWQGDEIFPFLETFEVAGDQSLIEITNRGQFGTRVLLTLAFTDVTAADLAQ
                     KLKLTKDIPTVALYRPGASVHKNGGGNVGLPHPLIEIRKDSFGKNGRIKLPLYMNDTK
                     GLTMRAVYSQRGQNLNWPAAAAPGVEKDGQGYWAVRR"
     gene            9013..>9412
                     /locus_tag="PLF85_06885"
     CDS             9013..>9412
                     /locus_tag="PLF85_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNE19424.1"
                     /translation="MAWRRQSILSAQQFTPQDLGEILELSRLLEERLRKTRNLKLLDD
                     YVLATLFFEPSTRTRLSFEAAMHRLGGRVISTVGVQFSSLAKGETLFDTLKMVESYSD
                     VCAIRHSVEGASTLAARSIKIPVINAGDGAG"
BASE COUNT         2132 a         2504 c         2609 g         2167 t
ORIGIN      
        1 gcggatcggc gggtctcgga agtcgagaca gatagcccgg tcggtatgca acaggtaaca
       61 aagccccgcc tcggccagca ggtacaggga gtggagctca tacacgctca ggccgccgat
      121 ttctttcacg acttcaatcc agtcctgtca ggtatttcgt tcagtatcag gactggacag
      181 cgcgtactgg tgacaggcag caatggctcg ggcaaaacaa cgctgctgag ggtattacac
      241 ggcgattttt atcccgccta tggctgcggg gatctgcagt tcaggggaga gctcgcgcat
      301 gaacagaaac gcgacctgtg gaagaaagtc cagttcatcg cggcagcgca cttcgactat
      361 tttttgcccg gtatgagcgt tgaggatgtt cttgcttcgc ggctttcggg ttcgctctat
      421 gactatgatc cggtgctacc cgatgcggcg ctgcaggtgg ctgagcagtt cgggctgttg
      481 cagtttctgc cgcgcccgtt tgcgaggctg tccgagggag aaaaaacacg cgttctgctt
      541 tgccgggctt tcctggcgcc ggccccggtc tacctgatcg acgagggctt tatggcgctc
      601 agcggtcgtt actttgaact ggtgacccag tacctgaacg cattgccggc agaagcggta
      661 actgtgatcg ctgccaacga acgcatcagc gcgctgcagg cacgactgga atttccctta
      721 ctccactggc aaatggagcg cgggcggctg accattcttc cttagagcgt ttcgaataga
      781 cgtagcgctc gagcttgctt tctttgacgt gcgcaatact taaaaagata aatccgtcga
      841 gtgcatttga gaaatccttg tcgatgccaa aatccaggaa gtgcacgcca ccttcttcgc
      901 agagttcggt gtactgcttg tagagcgtcg gcaccgcaaa tccgtagtgt ttcagattct
      961 gtttgagctc cttgaaactc tgcttgtatt cattaccgct aaaagcacgc gccaggcggt
     1021 cttctgcgcc ctgcgaaaaa cggaagcggt gtttagcccg cactggcgca tctttcttgc
     1081 caaaccagcg ctggtagaag tgaaccagca actcacgagc ctcgggggaa tacgagttac
     1141 tgactgaaac aggcccgaac aggtagttat actgtggcct tgccgccatg tacgcaccaa
     1201 taccgtgcca caggtaatcg agcgcctggc tattccagta gtttggctgt acgaagctgc
     1261 ggccaagctc aagtgattgc ggcaggtatt tctcaaattc ttcgctgaag cggaagagcg
     1321 ttgaagtata gaagccggaa atacctaatc ggcgatagat ttcgtcgcca gcggcaatac
     1381 ggtatgcacc gacaacttct agctggcttt cgtcccagag cacgatatgc tggtagtact
     1441 ggtcgaattc atcgaggtcg atcttgcgcc cggtgccttc gccgacttta cggaacgtca
     1501 gctcgcgcag gcggccaatt tccctcatga gggctggcgc ggcctcgtag cggacgagca
     1561 aaattttgat tttgtcttca gtctgaccaa gcagttctgc cgccataaca tcgttcagaa
     1621 tcagcttctt atccgttgga tgaacgatgt tcttggttgt acggaacgta ccgggtttgc
     1681 cttttccaat ctggtagaca tggcggcgca gcagctttgc ggcggccccg gctttgactc
     1741 ctgccagcga ttcaggcatg atcggatgcg atagtcttat ggcaatcgat cggtgtagct
     1801 ggctcttgat ttcggcgtcg agcgcgatat ccgagtacat acctccaacg gcagtccaga
     1861 aatagtgcgt gagccggttg cgcgcgccga tgtgaacagg gagcaccggt acctgccagt
     1921 gttgcgccat ctgtacaact gccttagacc agtctccgtc aacaaccagg cttaaacccc
     1981 agtgacggct ttttgccgga aagacgatga cagccttttc ggacgcgagc gcacggtgaa
     2041 tcgcccgaac cgtcgtgcgc tcagcgcctt tttgcgcgag tatgacatga ctgtctgttg
     2101 ctgcgaattc agagaggggc tcaaccactg ccacgacatc cttgcggata tcagagacca
     2161 cagtataaag ggctaccagg tcgagaaatg ccagtggatg gttggccaca atcaacagtc
     2221 ggccctgtgc cggaatgcgc tcccggtcgc gcaggctgag catcatcgaa aggtcgagtt
     2281 cgtcgcgcac gatctcgacc agatcgggca gtgggagttt tccgcttttc gtcaggtgcg
     2341 ctcgcagctt ttggaaagca cccgtataga gaatgttgtg cattttcata gtgtttatat
     2401 tttgccaatt gacgaattaa tggatatggg tctaatttcg gtcaattcgt caatctcatt
     2461 atgaagcgtc gcgcaatctg gtgtaacgcc gttggtctga tttcttcacg tgcaggtgca
     2521 gcaggtcaca aataagctgg cggtgttccc gcaggtaagt tttgcggctt tgcggcggag
     2581 gattaaagat attctgtttc tgcaccatgc gaaatggatt cttcttcagc tcctgccacg
     2641 ttccaatttc cagcgttaga ggcagaaaca ggttcttgcg cgcaatggat cccttttgtt
     2701 tcgacatttc aaattccagc agcgcacggt cccagaggtc tccgtgcgtc tggtaactca
     2761 gtgacatggg ctcgatgcgg taaagagggt gccggtcggc gagttcagct gccatatgct
     2821 gaaagaccga agcatgcggc actctccgtt tgctgtacga ataaggccac cagaggtagt
     2881 tctgtgcgcc ataacccgaa tggatgtcga gcgcggtatg caggcggccg cttttccagg
     2941 catactcgcg cagaaaccgg taaagggccc gcgattcgcg ttcgagtgct ctgccacggt
     3001 aatacggcag agccggcgaa aggcggtggc cgccgaagaa cggcagagct ccttctgcat
     3061 cgatgcccga attgcggttg agatcaaccc cccggccatt tgcccgggtc tcgcgtgcca
     3121 caccagcagg attcaggatg ggtagcgaat agatgccgaa tttaccttca gcgagcgcct
     3181 gcgagaaatg agaacttttg ggattcagaa agcacttgat catgtccagg tggatgcgga
     3241 taccaatagt ttcgaggccg tggacgccgg agacgatgct caccgcgtgg cgcttaaagg
     3301 cggagctctt accaatttcc agcaggtaaa tagggtaacg ctggccatcg cgggtcggac
     3361 tcgaaaagtc gagctgcctg atgcgtacca tgccgtctga gcgacgcacg agacgaacaa
     3421 ggcgccgctc ccagcggtca aaacgcagaa tctctttcgt ggtgcccaca gcacccgaga
     3481 cggaaaatct cacactttgt caattgacga aatgttcgcg gccagatatc tcccgttttt
     3541 ttttgcgtgt acgggtgtct cattatccga tcattaatca ggtgcgtcgg ctcttcctac
     3601 cgctgtttta ttttgtagcg aacgttatat cgtacggcgc ggctgcggct tcgagtgcgc
     3661 tctatggcgg aatcgtcact tttgtcttcg tgctttatta cagctacgcg atcaagttgc
     3721 cagcaataga tgaactgatt cttgtggtcg gaggtgttgc tgccttcacg actatcatac
     3781 acggcgttta tttcggtgtc ctgcacaaga ttggcatatc gggcatcaca cgcggtatgc
     3841 gcctgctcaa ccgtgcgatt gtgagaggga aaacctggtt cgaggtggat atcaggcaga
     3901 acctcaatgc cgacgagtac cgcgagctgc accgcatgct ctgttacctg cctcgcgaga
     3961 atgcctacat gtctgtcacg atggttctga tgattgttgg cggcgtgctg ctttatctct
     4021 accgctggca gaactacagc ttcatcaaca tggtgcaggt agtggtgatt gcagtcattg
     4081 cagcatttgt ccatgccggt tttacagcag tgattaccga actggttacc ggcgagatgc
     4141 gtacgcgagt caagcagatc atgtacgaaa agcgcgtgga attccaggaa atcgcgctct
     4201 ctaacgtgcg cgtgaagatt ttgctattta tcatgatcat gatcgcgacg ctttttgtct
     4261 cgaatttcat ggtttattac aatgtggaat tcccggtcct gatccgcttt tcggtttttg
     4321 ccatttttat cggcgccatc atggcctaca tgctgttctc gatcgtgctg cagtcactgc
     4381 atgaaattga ggtcgcctcg gtcagcctgc gcgacggcaa agatgcgcag ctttttccac
     4441 aggcgctcga ttctgaattt atcaatgtgg caacaggtct taacaccgcg gtgcacacga
     4501 tacgcgatta ccagcacaac ctcgagcgca aggtcgacga acgtacggcg gagctcactg
     4561 cggccaacga gtcactgcac gcgaaagacc gcctgatcca gatggagctc gattttgcgg
     4621 ccgagataca aaaagggatt attcccgcac agatcgaaga atggaacggg ctcaggtttt
     4681 acggttatta caagccgatg gaaaaagtct cgggggacta ctacgacgtc tttccaacgc
     4741 agggcaacaa actgggcata ctgatggcag acgtttcggg acacggcgtc ccggccgcgc
     4801 tgattaccac catggccaag gtagcgtttg cgcggtcagc cagcacgacc ccctcaccgg
     4861 ccaagacatt cgaagaggtc aacaaccagc tgctgggcct cgtcaccacg caggattacc
     4921 tgacggcatt ctaccttact atagatgaaa cgcatagctt cctctacggc aatgcctcgc
     4981 accagcaggc gaaaattgtc cgcaacaaga acattgaatc gctcgatacc ggtgggttgt
     5041 ttgtcggggc gatgcacgag gctaacgaga gttatgaaga gaaagaaggc cggctctacg
     5101 cgggcgaccg catgttcctc tacacggacg ggctgattga aatacgtaac ctggacgatg
     5161 aagaattcgg catcgagcgc ttcgatgcgt tgcttatcgc ggcatcagac ctaccgccgg
     5221 agcaggcagt ttcgttcatt gtggaagagg tctttcgctt cgcggctggc cgcaagccaa
     5281 acgacgatat ctcgatactg atgattgacg tgaaccgcga atactcccgt ttcttgcaga
     5341 ttgcctcgcg tgcttaccag gtgatcgagc agggcgaccg ccaggaaggt gtgcgtctgc
     5401 tcgacgaagc aattcgcctg tacggcagaa acccgctatc gctgcgcacg gcgggcgagg
     5461 taaattttga cctgggccgc atcgacacgg cggagcgtta cttcaaggcc taccttgatc
     5521 tgacacggca aaatgccgaa gttttctgct acctgagttc gatagcaatt ctgcgcacac
     5581 gctatgcaga agctgaaaaa tttgcccgtg aagcggtggc gctcagatcc aattacgcgc
     5641 tcgcctataa caacctggcc attgcctgcc tgaaccaggg aaaaaatgcc ctggcgcgtt
     5701 ttgcgatgga aaaggcaatt acctacgagc cggaaaacgc cgaattccag aaaaatgccg
     5761 ctcagctcga acgtgttttg ggcgagtaag caaaaataag tagccgccgc acccctgtca
     5821 tgcgtctaag taccatgcga aaggtacttt tcctgtcggc ttttttcgtt gcggtatttt
     5881 gcagcgaatc actgtttgca gcgcgcaaat ttgcgctgat tgtcggtaca aactacaagg
     5941 gctctgagat cagcccgctg gcgctctgcg aaaaagatgc tgaagtcatg aagcagtcga
     6001 ttgtaaagac cggcaatttt gacgctgcca atgtgcgtgt gctactgggc aaccaggtaa
     6061 cccgtgaaaa cctgcggtct tctatcgtcg aatggctcgg ccgcaacgtg aaagaaggcg
     6121 accaggtatt cttcttcttc gccgggcatg gacagtttat gcgtgacccg gcggccaaga
     6181 acggtatgcg caactatgtc atcatgttcc agcgcccgca cgtttcagac gatgaacttt
     6241 ccgaatggtt tggcaaactg cgcactaaga aggccgtgat tattctcgac tgctgtttct
     6301 cgggtggtat tgctaaaaaa ggagcagcaa cgcgtggcgc aggcaacgta ccgattcctg
     6361 aaggccagga ctacgcagtc ctgcaggatc tggaaaacgt gcacttccag aataaggtgg
     6421 tgatctcgtc ggctgacgac aatgaaacgg cgatcgagat tggcaaaccg atagagcacg
     6481 gcattttcac ttatttcttt agcaaagcga ttctcgaggc ggatctcaac ggtgacaaga
     6541 gtatcaccgc gtacgaagcc ttttacaagg cgcgtaacga gaccaccaaa ctcgcggcga
     6601 aagccaacca taaacaggtg ccgcagatca gcggcgacgc gtcaggtttt atgttcgtgg
     6661 cagctcccac accgacaacc ccgccgcagc cagtgctgcc gccggtacct gtaaccgtgc
     6721 ctgagccggt gatcgcgcct cccatcacac cggccgagcc acagaaccca cagaatacca
     6781 aaacaggtac gctcgtgatc aaaacgacct atacgcaggc acaactttcg ggcggcctgc
     6841 aggtatttgt cggccaggac gagtatgact tcaagctgag ttatgtcgaa tttggcgcat
     6901 ggggcaagac ggcgcagatc acgctcagca agatccccag cggtgtgcac aatgtcacca
     6961 tcaaggcggg cggttatgca gaccaggtca tcaagacggg tatcgaaccc aacggcacaa
     7021 cggttgaaac cgtgacctgc ggccttgctg gcaaaggcgc cattgcgggt aaggtatacc
     7081 tcgagacttt tgatcagcca gccgaggggt ttatagtgtt tatcaaacct ttaccgaact
     7141 tcaaacagcc cacttcaacc agccgcaaag acggtagttt tgcgttcaca gaagtgaaac
     7201 caggcaagta taccgtattc ctgcggggtt ctggcagggc gctgattaaa cagtacgacc
     7261 aggaaatcac cgtcgaaccc gaccgcatca ccaacctgga tattatcctg aggaatattt
     7321 ttaacaagaa gaagtaaaca aacccgcatc gggctgccac ttgggcagcc ctctacccaa
     7381 aacttgagaa atcagaaatc cgtcgatggc gactgcgcag atgccatcgg catgccttcg
     7441 cccgtatctg tttcgtcttc ttcgaaattc actgattcgg aagctgagtc ggcaaccaca
     7501 acaaaatcga tctggtcaat cacccggtta ttgtaggcaa ttttcagacg atactcgcct
     7561 tcttccaggt tgcggtagtg atcgataagc ataccctggc cctcggcgat ctgccggtta
     7621 cggaggtcaa tctcctggta accaaggctt tttttctgca gcgagatgcc gtagtaggcg
     7681 cctgactggg gcgatgcggg acggtaggcg taccagagaa catcgtcgcg ttcaaacacc
     7741 ggggtttgct ggtaagtgac aaaacgctgc gggtgaaaaa agcgctgctc gagcacgggc
     7801 aggttagtat ggtcagccac ggtgaaggcg aaccgtccac ggccattgca ggccatgaac
     7861 agcccggcaa tagtgacgaa caaaacactc cgcatactat attttcggtg cggcggagtg
     7921 attcttggta taaaaaaatt gcccgttagc ggcgcacggc ccagtaaccc tggccgtctt
     7981 tttcgacgcc aggtgctgcg gctgccggcc agttcaggtt ctgcccgcgc tgcgaataga
     8041 ccgctcgcat cgtcaggccc ttggtatcgt tcatgtagag cggtagcttt atacgaccat
     8101 ttttaccaaa cgagtccttg cggatttcga tgagtgggtg cggaagcccg acattaccgc
     8161 cgccgttttt gtgcactgat gcacctgggc gatagagcgc cacagtcgga atgtcttttg
     8221 tcagtttcag tttctgtgca agatcggccg ctgtaacgtc ggtgaaggcg agcgtcagca
     8281 gaacacgcgt accaaactgg ccccggttgg tgatttcaat caggctctgg tctcctgcca
     8341 cttcaaaggt ttccagaaag gggaaaatct catcgccctg ccagggcacc cagcgcatac
     8401 cattaaaacg gtagttgacc gcacccaggt tcagtaattc gtcgttcggt ttctcttcca
     8461 ctttagtcat cttaacagtc gcggtgtcag ccgtgtcggg cgtgaactcc tgcacaaagc
     8521 tgccgccatc gaagaggtgc agcgtctttt tatccgtacc atggatcacg agtacctgcg
     8581 acttcttatt accgacggcg atgaagaaga gtttgggttc gttgagcaag cctgcgaaag
     8641 catgcaggcg cagcagtgca taacctttgt ctgccggggt aaagatcacg aggtgcgtct
     8701 tgccctgtgc ctcgaacagc gtgatgtctt caaacgaggc gctgctgtcg agctggtact
     8761 ttttctgcac caggactttt tcgggtttaa cctttgccag ttcggcgatt tccgaggcag
     8821 agatgtgcgg gttcttgcgg cactgcgcca atacaagaag cagaacgggg ataatccact
     8881 tggtcatgga ggcttttctg caggacgaga ctggccggaa agccaaaggg aaagctgctc
     8941 atgacggtgg ctagcgggcg cgtagttccc ttacgcttga cgagacaggc gacataatgt
     9001 ttttcttcag ccatggcttg gcgcaggcaa tcgattcttt cggcgcagca gtttaccccg
     9061 caggatctcg gcgaaattct tgaactttcg cggctgctgg aagagcggct gcgcaagacg
     9121 cgcaacctga aattactcga cgactacgta ctcgcgacct tattttttga accctcgacg
     9181 cgtacccgcc tgtcgtttga agcggcgatg caccgcctcg gtggtcgggt catatcaacc
     9241 gtcggagtgc agttttcatc gcttgccaaa ggtgagacgc tgtttgatac gctgaagatg
     9301 gtcgaatcgt acagcgacgt ctgcgcgatc aggcactccg tcgaaggggc ctcaacgctc
     9361 gccgcgcgca gcattaagat tccggtcatc aacgccggcg acggggcggg cg
//
LOCUS       DAOQJF010000098         9398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3875_length_9398_cov_5.022691, whole genome shotgun sequence.
ACCESSION   DAOQJF010000098 DAOQJF010000000
VERSION     DAOQJF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_9398_cov_5.022691"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLF85_06890"
     CDS             complement(<1..533)
                     /locus_tag="PLF85_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19425.1"
                     /translation="MLRLFVEQAGHTAVVTADADFDTTEWLGHRFTWLNGVEKKTAVL
                     LSQAKGSADSRLRVTEADLAKSHKVLNATGIELAQLVANQYFSPLEEVIAQTLPPMLA
                     RTPGGSGKKTARKKAGSEQDQPQVHQVPELPAPELPSLSEKQQTVADAILSKPAGEAP
                     EHLIWGVTGSGKTRIYE"
     gene            complement(533..1939)
                     /locus_tag="PLF85_06895"
     CDS             complement(533..1939)
                     /locus_tag="PLF85_06895"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNE19426.1"
                     /translation="MPEQKKKSQKQSKATTAAKRSAADMSFHIETLGCPKNRVDSRRM
                     RTSLLRLGFREAQKPEKADFFLINSCSFIREAQEETIQTVFNALQVKEKKSPDMKVGL
                     VGCFVERFSQAVSQDIPELDFTIGTQRYHEVGGLITEQFGIEPAGHAAELSSEIMSLG
                     ADTQRPFAWLRIAQGCDRKCAFCIIPKIRGGLKTYSLEDIDRQIADELALRGSTVPLH
                     EVVLVSQDTISQGIDELEQIISHLSARADIRWIRLQYLFPDRRVLDLLDLWKKYPKLT
                     PYLDIPFQHVAPEMLKRMKRPSDVTLFDEIINKAHSITPDFEIRTSFIVGFPGETEAE
                     YQALVDFTRRHRLDKLALFRYSHEVGTSAGDTMAETVSDEEKYRRINELRDIHLETRK
                     QYALGLIGRRETMIVDEISGGEIVLRRAHDAPESDEVVTVPLTGDKQVKALTVGSMPV
                     VELKAYTEYSFLGEIVSP"
     gene            complement(1984..3093)
                     /locus_tag="PLF85_06900"
     CDS             complement(1984..3093)
                     /locus_tag="PLF85_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE19427.1"
                     /translation="MTAGQLLRKTREEKRIPLRQAADDTKITARHLQAIEDDNYMVFP
                     GETYALGFLASYAVYLEVDPEQVKRLYKGSQLTETETPLHELMQPTVQLSDYLEKFAK
                     PLIIIPIVLVAIAGIVMLVSHFSSGKDAGTSSVDSQTSAVTNFTRNSTTVPDVESDHV
                     KLQPGRPSPQIIKKGRAISFSVQNTEVFIVLRDIVSDEKTGNYSVGLEVYPGRKKYSL
                     KEEEPVDIEDSAIPRKFKVTLSGVTANNAKLVIDLGEEVQPAEGAPVTEVRVSNPDNF
                     LIRLEGVLTGDTYIEYFVDGKPGKRGLFKRDTPLLLEANDSIQIKIGDAGDVKLTING
                     KSEVLGPKGSVANKIIRKMRDPVEQSKFKVVIKDA"
     gene            complement(3096..3779)
                     /locus_tag="PLF85_06905"
     CDS             complement(3096..3779)
                     /locus_tag="PLF85_06905"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNE19428.1"
                     /translation="MKKTAYILAGLLITAPAHWADSFVHPSDVAKAMQAKFKALKAYQ
                     ADFSLSIKDLGKTRTSSGVATYGEGGKLNFTFSQPAGDIIVSNGQTLYVYVARLRAVG
                     RQPLKTKDKDGKSLYSAGTAEGLSNLFRRYHYRFDKPEQPRDFDGSRFFVLELKEKEI
                     TGGYDKITLYVEPETYLIRRMQASSPSGRSVELTFTNIKLNPAIDAKLFQFKEPENSK
                     IVDNPLTTE"
     gene            3997..5283
                     /locus_tag="PLF85_06910"
     CDS             3997..5283
                     /locus_tag="PLF85_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNE19429.1"
                     /translation="MDANAKKEISEEGLTELFNAALFHVRNDYVDDVSRQQLMFGAIR
                     GMLTALDDAHTRFMTAEETNELQTEMRGNFGGLGIEISQRDNVLTVVSPIEDTPAMRA
                     GIKPGDKIIEIDKKTTRDLSLSEAVKQLRGKPGTSVNISVVREGEDEILSFDLVREVI
                     KIQVVTSQFLEAEKLGYVRLKQFNQTATEDLAKALADFKKRKAKGVILDLRWNPGGLL
                     DAAHRISNFFIKSGVIVSTRGRKKELDRVFNADPSAAIAADLPLVVLANEGSASASEI
                     VTGAIKDHKRGKFVGVKTFGKGSVQNVISMMYGTSMALTIQKYYTPSGVSIHKKGIEP
                     DINVPALDFNKDDRRHYKEIREKKILQDFVKENKVYNAETVRKFQKMVAAKGLTLTDY
                     AAKRALKDELTKSENRPVFDLEFDVQLQRAITELKK"
     gene            5408..5743
                     /locus_tag="PLF85_06915"
     CDS             5408..5743
                     /locus_tag="PLF85_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE19430.1"
                     /translation="MEISQREKDAITILDIQGEIDLYNAPEIKDIIQKLIEAQKNNVI
                     INLEKVSYIDSSGIGALISSLSNLKKYQGGLKIINVYASVKKVFELTKLTSFFEIYES
                     EAEALAKFK"
     gene            5782..7125
                     /locus_tag="PLF85_06920"
     CDS             5782..7125
                     /locus_tag="PLF85_06920"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HNE19431.1"
                     /translation="MQKSKFRARAAMLISAAAMALVVGCGDPIPMEEMGVAKVAIARA
                     ETVKSAKYSAQNFDGAQKALLDAHNLIEQGKMSDAKEKAVEAKKLADAAYDESAPKLA
                     QETRGEAEVAIRSAEEANAEQFAADDLGAAKASLVQGDKYYESKDYLSSYHLFEESRE
                     KARKALTTSEAQVEVMKRDLAEIDDTIAAAERAGAAQSAPDTLKKAKDAAGHARGDLE
                     NKKLKSAYTNIEAAKVSSKEALVVAQKQSATEKLAQAKKDVAGAERKLNDLKARASSG
                     KGAKALEQSEEAQQALKTADENLVAAKEALNAAREAEKNQAYGEVITQSEEASRLAKV
                     LTDSLPETEVLLAQAKDRSSGAEGTGTEEGSSEETEKPAKAEDDSKAGWKTYKVRYIP
                     ENRDCLWKIAGYKFIYNNPRLWPKIYRANKAKIKNPDLIYPGQVFKIPPKKSKGD"
     gene            7258..8724
                     /gene="rho"
                     /locus_tag="PLF85_06925"
     CDS             7258..8724
                     /gene="rho"
                     /locus_tag="PLF85_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNE19432.1"
                     /translation="MARKKGADEEAAETTTEAPERGKIRLRGKKEGGEAPLAVEESPS
                     AAPQREAAPVSAPAANNPPRENGQNGKKMEISEFKDKSMNELVEMALSVGVENPADLK
                     KPALIFSILQKQSDNPGLVYAEGVLEVMPDGYGFLRSASYNYLAGPDDIYVSPAQIRM
                     FALRKGDTVYGQIRMPRDNERFFAILRVDTINGVPASEAMRRVHFDNLTPLYPEQLIK
                     LETTAEKLSTRVVDLVSPIGKGQRGLIVAPPRTGKTMLMQDIANAITTNHPEVTLLVL
                     LIDERPEEVTDMARSVRGEVIASTFDEPANRHVQVAEMVIEKAKRLVEHKRDVVILLD
                     SITRLARAYNQVIPSSGKVLSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDT
                     GSKMDEVIFEEFKGTGNMELHLDRKLVEKRTFPAIDMNRSGTRREELLLDKEDVNRIW
                     ILRKIFASLSTVESMELLLEKMRGTKNNKAFLDSMKTG"
     gene            9055..>9398
                     /locus_tag="PLF85_06930"
     CDS             9055..>9398
                     /locus_tag="PLF85_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19433.1"
                     /translation="MSDVERNYTGAGFLDTDTFQITCPLPASSEDRLGICRQKLITEL
                     VQYKERYDREAFARRMHQDFLPFFKPADVTDAEREEWRRNYHVMAEGRTRLVFERSTG
                     DGFEGVYRLRLK"
BASE COUNT         2233 a         2393 c         2625 g         2147 t
ORIGIN      
        1 tattcataga tgcgtgtttt acccgagccc gtcacgcccc agatgaggtg ttcgggtgct
       61 tcgcccgcag gcttgctgag aattgcgtct gccaccgtct gctgcttttc gctgagcgac
      121 ggaagctccg gtgcgggaag ctcgggtacc tgatgtacct gtggctggtc ttgctcgctg
      181 ccggccttct tgcgcgcggt ctttttcccg ctaccgccgg gtgtccgtgc cagcataggc
      241 ggtaaagtct gcgcaattac ttcttcgaga ggagaaaaat actgattggc gacgagctgc
      301 gcgagttcaa tcccagtggc gttcaatacc ttgtgggatt tcgcaagatc tgcttccgtg
      361 acgcgcagtc gcgaatcggc cgaacctttc gcttgcgaaa gcaggactgc ggtttttttc
      421 tctaccccgt tcagccaggt gaaacggtgg ccgagccatt ctgttgtgtc aaagtcggca
      481 tcggccgtga caacagccgt gtgtcctgcc tgttcgacaa aaagccggag catcagggag
      541 aaacaatttc acccaggaat gaatactctg tataggcttt gagctccacc accggcattg
      601 agcccacggt gagcgctttc acctgtttgt cgcccgtcaa tggtacggtg acgacttcat
      661 cactttcggg tgcatcgtgc gcccggcgca gcacaatttc gccgccgctg atttcatcga
      721 cgatcatcgt ttcgcggcgg ccaatcagcc ccagcgcata ctgtttgcgg gtctcgaggt
      781 ggatatcgcg cagttcattg atgcggcggt atttttcttc gtcgctgaca gtttcggcca
      841 ttgtatcgcc ggcgctggtg cctacctcgt gcgagtagcg gaacagcgcc agtttgtcga
      901 gccggtgccg gcgcgtaaaa tctaccagcg cctggtactc agcctcagtt tcaccgggaa
      961 atcccacgat gaacgaagta cggatttcaa agtcgggcgt aatggaatgc gccttattga
     1021 taatttcgtc gaagagcgtt acgtcagatg gacgtttcat gcgcttgagc atttcgggtg
     1081 caacgtgctg gaacgggata tccaggtagg gcgtgagttt gggatatttt ttccacaggt
     1141 cgagcaggtc gagcacgcgc cggtcaggaa aaaggtactg caggcgaatc cagcggatat
     1201 ctgcgcgcgc agaaaggtgt gagataatct gctcgagttc atcgattccc tggctgattg
     1261 tatcctggct gacgagtaca acttcatgca ggggcacggt actgccgcgc agagcaagtt
     1321 catcggcaat ctgccggtcg atatcttcga gcgagtaggt cttgagcccg ccgcgtattt
     1381 tcgggatgat gcagaaggca cacttgcggt cacagccctg tgcgatacgc agccaggcaa
     1441 atggccgctg cgtgtctgcc ccgaggctca tgatctctga cgagagttct gcagcatgtc
     1501 ctgcgggttc gatgccgaat tgttctgtga taaggccgcc gacttcgtgg tagcgctgcg
     1561 tgccgatggt aaaatcgagc tccggaatat cctgcgagac ggcctgcgag aagcgttcga
     1621 caaaacagcc gaccaggcca accttcatgt cgggcgattt cttttctttt acctggagcg
     1681 cattgaagac cgtctgtatt gtctcttcct gtgcttcgcg gataaaagag caactgttga
     1741 tcaaaaagaa gtcggctttt tcaggctttt gcgcctcacg aaagcccagg cgcaatagtg
     1801 acgtgcgcat gcgccgcgaa tcgacccggt tcttggggca gccgagtgtt tcgatgtgaa
     1861 agctcatatc tgccgcagat cgttttgcag ctgttgtggc ctttgactgt ttctgggatt
     1921 tctttttctg ctcgggcatt ctgcctgcaa tatagccacg ggcagggccc gtggcttatg
     1981 tttttaagcg tcttttatga ctaccttgaa ctttgactgc tcgaccgggt cgcgcatctt
     2041 gcggataatc ttgttggcta cgcttccctt gggtccgaga acttcgcttt ttccattgat
     2101 ggtcagtttc acgtcaccgg catcgccaat tttgatctgg atgctgtcgt ttgcctcaag
     2161 tagcagcggt gtgtcgcgct tgaagaggcc ccgcttgccg ggcttgccat cgacgaagta
     2221 ctcgatatag gtatcgcctg taaggacacc ctcgaggcga atgagaaagt tgtcagggtt
     2281 cgagacgcgc acttcagtga ccggtgcgcc ctcagcaggc tgcacttctt caccgaggtc
     2341 aatcaccagt tttgcgttgt tggcggttac gccggaaagt gtcaccttga acttgcgcgg
     2401 gattgccgaa tcttcaatgt cgaccggctc ttcttctttc agtgaatact tcttgcggcc
     2461 gggataaacc tcaaggccca ccgagtagtt gcccgtcttt tcatcggata cgatatcgcg
     2521 cagaacgata aatacctcgg tgttctgtac cgagaagctg attgcccgtc ctttcttgat
     2581 aatctgcggg ctagggcggc caggctgtag tttgacgtgg tcagattcaa catcaggcac
     2641 ggtggttgag ttgcgggtaa aattggtcac cgcgctcgtc tggctgtcca cgctggaagt
     2701 gccggcgtct ttaccagacg agaagtggct caccagcatc actatgcccg ctatcgccac
     2761 caggactata gggataataa tcagcggctt ggcaaatttt tccaggtagt cgctcagctg
     2821 taccgtgggc tgcatgagtt cgtgcagtgg agtttcagtt tcggtgagct gtgacccctt
     2881 gtagaggcgc tttacctgct ctggatcaac ctcgaggtat actgcgtagc tggcaagaaa
     2941 gcccagcgca taagtttcgc ccggaaagac catgtaattg tcgtcttcga ttgcctgcag
     3001 gtggcgcgct gtgatcttcg tgtcatcagc ggcctgacgc agggggattc gtttttcttc
     3061 ccgggttttt cttaagagct ggcctgctgt catgcttact ccgtcgtcag tggattatcc
     3121 acaatcttgg aattttccgg ttctttgaac tggaaaagtt ttgcgtcgat tgcggggttt
     3181 agtttaatat tggtaaaagt cagttcaacg ctgcgcccgc tgggcgaaga agcctgcatc
     3241 cggcggatca ggtatgtttc tggttctacg tatagcgtaa tcttgtcata accaccggta
     3301 atttctttct ctttcagttc cagaacaaag aaccggctgc catcaaaatc ccgtggttgt
     3361 tcaggtttgt cgaaccggta gtggtaacgg cgaaagaggt tactcaaacc ttctgcagtc
     3421 ccggcgctgt agagcgattt gccgtctttg tcctttgtct tgagaggctg gcgacccact
     3481 gcccgcaggc gcgcgacata gacgtaaagg gtttggccgt tgctcacgat tatatcgcca
     3541 gctggctggc tgaaagtgaa gttcaatttg ccaccttcgc cataagttgc tacgccagag
     3601 ctagtgcgtg tcttgccgag gtctttgatg ctcagcgaaa aatctgcctg gtacgctttg
     3661 agcgccttga acttcgcctg catggccttg gccacgtcag aggggtggac aaaactgtcg
     3721 gcccaatgtg caggtgctgt aatcagtagt ccagccagta tgtacgcggt ttttttcata
     3781 tgaattgccg gggattttcc gagaaaattt cgtttgagaa acagggtgtc aaacaatatc
     3841 aaagggaaaa atcattgacc ggcaattgag ctaaccgatg ccacccacga ccttatcatg
     3901 agtaacccca atacctccgc cactgcggat cgccggaaaa aatatcttgg cctgattctt
     3961 tgtaccgggc tgttcgttgc cgcaacagcc gggcaaatgg acgccaacgc gaagaaagaa
     4021 atatcagaag aaggcttaac cgaactcttc aacgccgcgc tgttccatgt gcgcaacgac
     4081 tatgttgacg atgtttcgcg ccagcagctg atgtttggtg cgatacgcgg tatgctcacc
     4141 gcgttagacg atgcgcacac gcgcttcatg acagcagaag agaccaatga gctgcagacc
     4201 gagatgcgcg gtaattttgg cggtctcggt attgagattt cgcagcgcga taatgtgctt
     4261 actgtggttt ctccgatcga agatactccg gcgatgcgcg ctggtatcaa acccggtgac
     4321 aagatcattg aaattgataa gaaaactacg cgtgacctgt ctctcagcga ggcggtgaag
     4381 cagctgcgcg gcaagccagg cacttcggtc aacatcagcg ttgtacgcga gggtgaagat
     4441 gaaatcctct catttgacct ggtgcgcgaa gtaattaaga tacaggttgt aacttcacag
     4501 ttcctcgaag cagagaaact tggctacgtg agactgaagc agtttaacca gacagcgaca
     4561 gaagacctcg cgaaagcact ggcagacttc aagaagcgca aggcaaaggg agttatcctc
     4621 gatttgcgct ggaatcctgg cggcttgctt gatgccgctc accgcatcag caattttttc
     4681 ataaaatcag gagtgatcgt ttcaacccgt ggccgcaaga aagagctcga tcgcgtattt
     4741 aatgctgacc cttcagccgc gatcgccgca gatctgcccc tggttgtgct cgcgaatgaa
     4801 ggttctgcct cggcaagtga aatcgtcacg ggggcgatca aagaccacaa acgcggcaaa
     4861 tttgtcgggg tgaagacctt cggcaagggg tcggttcaga atgtcatcag catgatgtac
     4921 ggcacctcga tggcgctcac catacagaag tactatactc cctcaggagt ctcgatccat
     4981 aagaaaggta tcgagcccga tatcaatgtg ccggcgcttg acttcaacaa agatgatcgc
     5041 cgtcattaca aagagatccg tgagaagaag attctgcagg atttcgtgaa ggaaaacaag
     5101 gtttacaatg ccgagacggt gcgcaagttc cagaagatgg tggcggcgaa agggctgacc
     5161 ctcacggact atgcagcgaa gagagctctc aaagatgagc tgaccaagag cgaaaatcgg
     5221 ccggtttttg acctcgaatt tgacgtgcag ttgcagcgcg ccatcactga gcttaaaaag
     5281 tgagtcgtgc acggctgggg gaatgcttta tgccggcctg tctgcccagt caaatgttgt
     5341 tttttgattg acgataaaaa acgtgttaag gatgcgttaa taatataatt taaccggagc
     5401 caatgccatg gaaatcagcc agagagaaaa agacgctatt accattctag atattcaggg
     5461 ggaaatcgac ctctacaacg cgccggagat caaagatatc atccagaagc tgattgaagc
     5521 gcaaaaaaac aatgtcatca tcaacctgga aaaggtttcg tacattgact cttcaggtat
     5581 cggggccctg atttccagtc tctcaaacct gaagaaatac cagggcggac tgaaaatcat
     5641 caacgtctat gcctcggtaa agaaggtttt cgaactcacg aagctcacga gcttttttga
     5701 gatttacgag tcggaagcag aagcgctggc aaaatttaaa taatgaaatt caatatttcc
     5761 tatcaactca ccggagcaac catgcaaaaa tccaaattcc gcgcacgcgc agcgatgctt
     5821 atctcagctg cagccatggc acttgttgtt ggttgcggcg acccgattcc gatggaagaa
     5881 atgggagttg ctaaagtagc gattgcccgt gcagaaacgg tgaagagcgc taaatactct
     5941 gcgcagaact ttgatggcgc acagaaagcg ctgctcgatg cacataacct gatcgagcag
     6001 ggtaaaatga gcgacgcgaa agagaaggcg gtagaagcca agaagctcgc tgatgctgca
     6061 tacgacgagt cagctccaaa gctggcacag gaaacgcggg gcgaggcaga agtggcaata
     6121 cgctctgcgg aagaggcaaa tgctgagcag ttcgcggcag atgacctggg cgctgcaaag
     6181 gcgagccttg tgcagggcga taaatactac gaatcaaaag attatctgag ctcctaccac
     6241 ctgttcgaag aatcgcgcga gaaagcccgt aaggcgctca ctacttctga ggcgcaggta
     6301 gaagtcatga agcgtgacct cgccgagatc gatgacacta ttgctgccgc cgaacgtgcc
     6361 ggtgcagcac agagcgcccc ggatactctc aaaaaagcaa aagatgcagc tggtcatgcg
     6421 cgcggcgacc tggaaaacaa gaagctaaaa tctgcgtaca caaatatcga agctgcaaaa
     6481 gtttcttcta aagaggcact tgtggttgcc caaaaacagt ctgctactga aaagctggca
     6541 caggcgaaga aagatgttgc cggcgcagag cgcaaactga acgacctaaa agcgcgtgca
     6601 agcagcggca agggcgcgaa ggcgctggag cagtctgaag aagcacagca ggcactgaag
     6661 acggctgatg aaaatctcgt agccgctaaa gaagccctca acgcagcacg cgaagcagag
     6721 aagaaccagg catacggtga agtaatcacg cagtccgaag aagcgagccg cctggcaaag
     6781 gttctgacag attcattgcc ggagaccgaa gtactgctgg cgcaggccaa ggacagatca
     6841 tctggtgcag aaggtactgg aactgaagaa ggttcctccg aagaaactga gaagcctgca
     6901 aaagctgaag atgacagcaa ggctggctgg aagacctaca aagtgcgcta cattcctgag
     6961 aaccgcgatt gcctctggaa aattgccgga tataagttca tctataacaa tccgcgcctg
     7021 tggccgaaga tttaccgggc caataaagcc aagatcaaga accctgatct gatctaccct
     7081 ggccaggtgt tcaagattcc gccgaagaaa agcaaaggag actaatccca ttacggccgc
     7141 gctggcgcgg cctcaaatat tcagcaccgg ttccgtccgg aaccggttac caaacgtcat
     7201 caatctcacc cccagcactt aggctggtaa ttcaccctag aaactcaaga ggaaacaatg
     7261 gctcgtaaga aaggcgcgga cgaagaggcc gcagaaacca ccacagaggc tcccgaaaga
     7321 gggaaaatac gcctgcgtgg aaagaaagag ggcggtgaag cgccactggc tgtagaagaa
     7381 agcccatccg cggccccgca acgtgaagcg gcgccggtgt ctgcccctgc tgcgaacaac
     7441 ccgccgcggg aaaatggcca gaacggcaag aagatggaaa tcagcgagtt caaggataaa
     7501 tcaatgaatg agctggtgga gatggcgctc tcggtcggcg tggaaaaccc cgctgacctc
     7561 aagaaacccg cgctcatttt ctctatcctg cagaaacaat cggacaaccc cggcctggtc
     7621 tatgccgagg gtgttcttga agtgatgccc gatggctatg gctttttgcg ctctgcgagt
     7681 tacaactacc ttgcgggccc ggatgacatc tatgtttcgc cggcgcagat tcgcatgttt
     7741 gccctgcgca agggtgatac cgtttacggc cagattcgta tgccgcgcga caacgagcgg
     7801 ttcttcgcga ttctgcgcgt tgatacgatc aatggcgtgc cagccagcga agcgatgcgc
     7861 cgcgtgcact ttgataacct gacgccgctg tatcctgagc aactgatcaa actcgagacg
     7921 actgctgaaa aattatctac gcgcgtggta gaccttgtgt cgcctatcgg caaaggccag
     7981 cgcggtctga tcgtggcgcc accgcgcaca ggtaagacga tgctcatgca ggacattgcg
     8041 aatgccatca ctaccaatca ccccgaggtg acgctgctgg tgttattgat cgatgaacgt
     8101 ccggaagagg tcacagacat ggcgcgcagc gtgcgcggtg aggtgattgc ctcaaccttc
     8161 gacgaacccg cgaaccgcca cgtgcaggtc gctgaaatgg tcattgaaaa ggctaagcgc
     8221 ctggttgaac acaagcgcga cgtagtcatt ctgctcgatt cgataacgcg cctcgcacgc
     8281 gcttacaacc aggtgattcc ctcttcgggc aaggtgctct cgggtggtgt cgactcgaac
     8341 gcgctgcata aaccgaaacg tttcttcggt gccgcacgga atatcgaaga gggcggctcg
     8401 ctgacaatta tcgccaccgc gctgattgat accggtagca agatggatga agttattttc
     8461 gaagagttta aaggcactgg taacatggag ctgcacctcg accgcaagct ggtcgagaag
     8521 cggacattcc cggccatcga catgaaccgc tcgggcacac gccgcgaaga gttgttgctg
     8581 gacaaagaag acgtgaaccg tatctggatt ctgcgcaaga tattcgcctc gctgtcgaca
     8641 gtcgagagca tggagctact gctcgaaaaa atgcgcggta cgaagaacaa caaggcgttc
     8701 ttggatagta tgaagaccgg ctagcatcgc ccgaaaatgc ggtgtttttt gacagccgta
     8761 agcaggcctt taggccgctt tagcagccgt ttccctcagg aaacggcgag gcttacggct
     8821 gtcccgcatt tttggagcga tggggctagc tttggtctgc gttttttcgt aacgggaact
     8881 gcgcgtttct gcaaggcgcg ggctagcgtg tcgttgttgt gtattctgaa atcggctgtc
     8941 ccacattttc ggagcgatgg ggctggcaaa ccccgggctt ttttcagtac attccttttt
     9001 ctccctttcg cgtttctctt cctcatcgca tgcgctccct cgaaggacac ggtgttgagt
     9061 gatgtagagc gtaactatac cggagcgggc tttctcgaca ccgatacgtt ccagattacc
     9121 tgtcctctgc ctgccagtag tgaagaccgt cttggcattt gccggcaaaa actcattacc
     9181 gaactggtgc agtataaaga gcggtatgat cgcgaggcgt ttgcgcggcg catgcaccag
     9241 gacttcctgc cgttttttaa gccggctgac gtgactgatg ctgagcgcga agagtggcgg
     9301 cggaattatc acgttatggc agaagggcgc accaggctgg tgtttgagag gtcaacgggt
     9361 gatggtttcg agggcgtcta tcgcctgcgg ctcaaaga
//
LOCUS       DAOQJF010000099         9390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3880_length_9390_cov_4.646063, whole genome shotgun sequence.
ACCESSION   DAOQJF010000099 DAOQJF010000000
VERSION     DAOQJF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3880_length_9390_cov_4.646063"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLF85_06935"
     CDS             <1..822
                     /locus_tag="PLF85_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19434.1"
                     /translation="IANPAYHAADYIVLPASLPPQAKKPAQNAAEEKKAAGWLYEPKV
                     KKKLPRVRLATFSWARLSVALLIAAGGASAFLLYSRYTQQPVRPEIPALQVADSAAVM
                     PVGKGARVLYTYRSRDAIITDFEKAKQLLGERKVNQSRFILQRLIESNADFQSREKSR
                     IFLNFIPDLPFAEFNDNILLRDIFADTKARMGSLILLAGELRDAATRSGATTYHFMVR
                     EGEREYLVQAFRDGNLPEEKLQSSDKKSHIQIYGRFRGLVGEQKTIYLEALRVWK"
     gene            1147..1749
                     /locus_tag="PLF85_06940"
     CDS             1147..1749
                     /locus_tag="PLF85_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19435.1"
                     /translation="MGLQQSRIKSAELQLANARKNLADQQARIKATEEYVNSIKEEIK
                     TLEAENSPQNAAKLNRAIRMLNLYSNNLTIYAADLKKMEQFETTAIAKGAAAKADLAA
                     IAAEEKALIAQLQKSQPTLIPQGDSAVATSDITPPLESEKKKVRPAGLTTVGQTGSEQ
                     SISEQNDVKAGVGNDTQQLYNNVNTVTQPTTTGQPTLRKL"
     gene            1767..3221
                     /locus_tag="PLF85_06945"
     CDS             1767..3221
                     /locus_tag="PLF85_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNE19436.1"
                     /translation="MKKMYKMMAFAAGAVFTLAAEAQPKVTGSASADSTAETKASSVV
                     EETRAGEHFLRAGMLATTNYYQDQVLKSGLVNGNYRPFASYSYNDKYTVTVRGNLALK
                     YYPEKPAGVEKQTNAVGILEITSFEGNFGKSSVTAGRSFYQTEQGILFANFADGVAYN
                     GSYSFGKVKAMGLYSADYGKSACSININGCGGDPNPFNTIPGLQPDASVTGSGQRLFA
                     TLEYTTRAFDFGPLNVTSMVYGVYSKDFIKESPSVVTRYEYNPYYAGVGFLGYIINAK
                     LQYRVDGIYQGGESYNLSANGESLQNKIMVGALISRMNYVLPVLTTIDTQVSAEYTVA
                     SGDADASRAGTASQSNTAGSYTAFQTFGAYSGGLALKPRLTNMQIFRGGFELRPLKMF
                     FWARNIGLSVKYSVYRKVVATGGISDGAATEASTDIGMAGDAGINFAARSDLQFFYGF
                     GIFKPGDAYPVLASDGSDGRALRQAHIVSLTLVF"
     gene            3358..3864
                     /locus_tag="PLF85_06950"
     CDS             3358..3864
                     /locus_tag="PLF85_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19437.1"
                     /translation="MSPAKLSGKTSLRLADGSPISQASTDEYNPYLVKMADGYLVLVF
                     GSDRACAGCSAGTHNLFLARSVSVYNDDAKLPTFNAPVAFTVASNPVNDGGRISFAAV
                     RNSAQLRIYLMSSGIIKYADVNTAGAPYNVAALTSIAHFGLSRTTLLGIDATGSKLVA
                     RASGAAHT"
     gene            3907..4995
                     /locus_tag="PLF85_06955"
     CDS             3907..4995
                     /locus_tag="PLF85_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19438.1"
                     /translation="MTVLYAGSKEGTLVALSAAGAGGTLDDLYILEGTSAGMLWEQQS
                     SKPQSGAATAPVWVQPQLTATRVYGQNGLFTCAVVNNNNSGCTSGGGTPNDKSLYRGS
                     RPAISDDGLYIADQLNNRALFFSGISTTATRVYCQAGSFTTATFGTVPGSQTCRGNPS
                     YGNVSSVFVDSSGVYVNDIEANRVLFFAGANIDAASGLGGRIYGQGGNYTTFAAGTTA
                     QTFTNPDTTTSDIDGIYIADNGNNRVLFYPGTSTTATRVYGQSNMTSNTAGGGASSLN
                     WPWGVKYFGGGIYVVDYNNHRVLFFPENSGTATHVWGQNGSFTANSANQGGSVSATSL
                     NNPYAVDVDHTGMYVGDNQNHRVVFYPR"
     gene            5111..6253
                     /locus_tag="PLF85_06960"
     CDS             5111..6253
                     /locus_tag="PLF85_06960"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE19439.1"
                     /translation="MKLAFKRPPVGRTQWVSIAFLSLSPVIGFGALAYFLHTGTLSSA
                     TVTLAVVIATIAGMGTTAGYHRLFSHRSYEAALPVRVFMLLIGLLGLQGSALEWSLDH
                     RTHHKFVDRDKDPYSITKGFWFAHMLWIFKTRPRDWRPSHNADLFRDKFVFWQHRFFM
                     PLAVVANFILPAAVAGLFWGDLWGGLLIAGFLRLVFTHHSTFFINSLCHIAGSQPYSN
                     KHTARDNWITAFFTWGEGYHNFHHEFANDYRNGVRWYQYDPGKWLIWLLSHLHLASNL
                     KRVPDTTIRQRCMQMQKGELLSKLEKWGWTEERKQLFRAQLEETFAAFRRRATVMNEI
                     RNRMKSDAVQATQRRFELMQERYERLQDAVEETAVQWKLLRRRARI"
     gene            complement(6299..7471)
                     /locus_tag="PLF85_06965"
     CDS             complement(6299..7471)
                     /locus_tag="PLF85_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE19440.1"
                     /translation="MIEWSEKLSSVVQTLKPSGIRQFFDIVATRKDCISLGVGEPDFV
                     TPQVVIDHSIQALRDGYTHYTGNSGFLSLRREIARYLEKEYNLSYNPEKEILITVGVS
                     QGVDLAFRALLNPGDGVLYPEPCYVSYDPMIRLAGGIPQKVLATAENDFSLKAENISG
                     VVHAGGVQSKVLFLNYPSNPTGASIGADELRRIARLAEEKNLIVISDEIYGELNYEGE
                     HVPFPTLPGMKERTLLLGGFSKAFAMTGWRIGYACGDENLIKSMTKIHQYSMLCAPTL
                     GQIAAETALKYALADRDRMRDEYRLRRDLIVKGFNDMGLKCFMPQGAFYVFPDIRSTG
                     KNSMQFATELLEAQNVAVVPGTAFGDSGEGFIRCSYATARKEIEAALTKIAAFVKS"
     gene            complement(7518..8714)
                     /locus_tag="PLF85_06970"
     CDS             complement(7518..8714)
                     /locus_tag="PLF85_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNE19441.1"
                     /translation="MGLPSIEQAEVSGKKVLLRVDFNVPLDKATGAISDDTRMVKSLP
                     SIELLLNRGASVIIISHLGRPEKKGDPALSLKNIAAHLGTLLPGRTVNFASEAVGPVA
                     EELAGKLKAGEILVLENIRYYDEETSKEEKVRNDFAKKLCKLADIYVNDAFGAAHRAH
                     ASIYECAKLLPGFAGLLLKKEIDILQKLVTDPPRPFVAIIGGSKVSSKLAVLENLVTK
                     ADSILIGGAMAYTFLKSRLIEVGTSMIEKDYLSQAFQIIDKAAYNKRELLLPEDHIVS
                     SEFGEKGKIKTVKKSIPDGFMGMDIGPKTADHYAKVIKNAKTVLWNGPMGVFEMKKFS
                     GGTFAIAKAMAKVKGSTIVGGGDSIFALTQSGLEDKMTHVSTGGGATLEYLEGKKLPG
                     VEALLK"
     gene            8834..>9390
                     /locus_tag="PLF85_06975"
     CDS             8834..>9390
                     /locus_tag="PLF85_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19442.1"
                     /translation="MAETQDKGRSRKALGLLFEQALEAEKRGELRTALEKYKMALQID
                     QNFFDAWLNAGAIYTRQGRSDKAIVCYQRALVSKNDVRAHYNLAVEFYKREDFAGAEK
                     ALRSVLKLDSRYLNAHLLLGYVFGKTGRNDKSEISIRNALKIDPQNVQAQTALALLYF
                     HTDRHELAQRQIQLLLNKSPEDKVLL"
BASE COUNT         2138 a         2532 c         2579 g         2141 t
ORIGIN      
        1 attgcgaacc ccgcgtacca cgcggccgac tatatcgtgc tgcctgcaag tctgccgccg
       61 caggcgaaga agccggcgca gaatgcagct gaagaaaaaa aagcggcggg ctggctgtat
      121 gagcctaagg taaagaagaa actaccccgg gttcgcctcg caactttctc gtgggcccgc
      181 ttgtcagtcg cgctgttgat tgcagctggg ggagcttctg ctttcctgct atacagccgc
      241 tacacgcagc aaccggtgcg gccggaaatt cccgccttgc aggtagcaga ttctgccgcc
      301 gtgatgcctg tgggcaaagg tgcacgagtg ttgtacactt accgttcgcg tgacgcgatc
      361 atcactgatt ttgagaaagc caaacaattg ttgggcgagc ggaaggtgaa ccagagccgg
      421 ttcatcctac agcgcctgat tgagtccaat gcagatttcc agagccgcga gaagtcgcgt
      481 atttttctga atttcatacc cgacctgcca tttgccgagt tcaacgacaa tattctgctg
      541 cgtgatattt ttgccgatac gaaggctcgt atgggcagcc tgattttgtt agccggtgag
      601 ttgcgcgacg ctgcgacgcg ctcgggtgcg acaacctacc attttatggt acgcgaaggc
      661 gagcgcgaat atctcgtgca ggcattccgt gacggcaatc tgcctgaaga gaagttgcag
      721 agttcagata aaaaatccca catccagata tatggtcgtt ttcgcggtct ggttggagaa
      781 cagaaaacta tctaccttga agcgttgcgt gtttggaagt aggtataata tccgagggca
      841 gtttttcggt taaccatcgt agataattca gttgcgccct gcgctcagtc tattgtctga
      901 ccctttttgc aggtactttg aatgcggagg cgcgatttgc gtcgctgaaa gggacggttc
      961 agatttttga gaaccgtaaa tgggttaacg cgactccgca gaccaaactt gcttcaggaa
     1021 cgatgattca gacctcttac cgaagtcagg cagtcgtcat ctaccctaag ggcggccagt
     1081 tagctgtcgg accgaatacg cgggtcacca tctttgataa cgcaaccggt tcaggcacag
     1141 atcgagatgg gtctgcaaca atcacgcatc aaatcagctg aactacagct tgccaacgcc
     1201 cgcaagaacc tcgctgacca gcaggcgcgc ataaaagcta cggaagagta cgtaaactca
     1261 attaaagaag aaatcaagac acttgaggcg gaaaattcgc ctcaaaatgc ggcaaagctg
     1321 aaccgtgcca ttcgaatgct aaatctttat tcgaacaacc tcacgatcta tgctgcagat
     1381 ttaaagaaga tggaacagtt cgaaaccaca gcaattgcca aaggcgctgc agcaaaggct
     1441 gacctggctg ccattgcggc tgaagaaaag gcccttattg ctcaactgca aaagagtcag
     1501 cccacgctga ttccgcaggg tgactctgcg gtagcgacaa gtgacattac ccccccactc
     1561 gaatcagaaa aaaagaaggt tcggccagca ggtctgacca cggtgggaca aaccggtagc
     1621 gagcaatcca tttctgagca gaatgatgta aaggccggcg tcggtaatga tacccagcaa
     1681 ctgtacaaca acgtcaatac agtgacgcag cccactacca ccgggcaacc gacactcaga
     1741 aagctctgag acagcgcttc tccaggatga aaaaaatgta taagatgatg gcattcgcgg
     1801 ctggtgccgt gtttacgctc gctgctgagg cgcaaccaaa agtaaccggt tcagcgtcgg
     1861 ccgacagcac ggcagagaca aaggcctcgt ccgtggttga agagacgaga gcaggtgagc
     1921 acttcctgcg cgcaggcatg ctggcaacga ccaattacta tcaggatcag gtattaaaga
     1981 gtgggcttgt gaatggtaac tatcgcccat ttgcgagtta ttcgtataac gataagtata
     2041 cagttacggt acgcggtaac cttgcattga agtattatcc tgaaaagccc gcgggagtgg
     2101 aaaaacagac aaacgctgtg ggaatactcg aaataacttc tttcgagggc aattttggca
     2161 aaagtagtgt gacagccggc cggagctttt accagacaga gcagggtatt ctctttgcca
     2221 attttgccga tggcgtagca tataacgggt cgtattcgtt tggtaaggtc aaagcgatgg
     2281 ggctctattc cgctgactat ggaaaatccg catgttccat caacatcaac ggctgcgggg
     2341 gagatcccaa cccgttcaat accattccgg gcttgcagcc cgacgcttcg gtgacaggtt
     2401 caggccaacg tctgtttgcg acgttggaat acacaacccg cgcgttcgat tttgggccat
     2461 taaacgtgac cagcatggtt tacggcgttt acagtaagga cttcatcaaa gaaagcccca
     2521 gtgttgtgac gcggtacgag tataatccct attacgcagg cgtgggtttc cttggttata
     2581 ttattaatgc caaactgcag tatcgcgtag acggcattta ccagggtggg gaatcgtata
     2641 acctgagcgc gaacggcgaa tcgcttcaga acaagattat ggtgggggca ctgatcagca
     2701 gaatgaacta tgttcttcct gtcttgacaa caatcgatac gcaggtttcg gccgagtaca
     2761 ccgtggcctc aggtgatgcc gatgcatcgc gtgcgggtac cgcttcgcaa tccaatacag
     2821 cgggcagcta cacagcattt caaaccttcg gcgcttattc aggcggtctg gcgctcaagc
     2881 cgcgcctgac aaacatgcag atatttcgcg gcggttttga gctgcgccca ctgaagatgt
     2941 tcttctgggc gcgcaacatt ggcctttccg ttaaatactc ggtttatcgc aaggttgttg
     3001 ctaccggggg catttccgac ggcgcggcga cagaggcttc tactgacatc ggtatggcag
     3061 gcgatgcggg tatcaatttc gccgcgcgca gtgacctgca gttcttctat ggctttggga
     3121 tatttaagcc gggcgatgcg tacccggttt tggcgagcga tggttcagat gggcgtgcgc
     3181 tgagacaggc gcacattgtt agtttaacat tggtgtttta agtacctctc ccgtccttcc
     3241 cggcatgcgg ggaaggtgtc cagatggcgg tggaggatgc aaggagttgc aatgagagga
     3301 atgaagttac tggctggatt ttccatgctt ttggggctga tagcctgcag gggcgatgtg
     3361 tctcctgcga aactcagcgg caaaacatct ctgcgcctcg ccgatggctc accgatttct
     3421 caggcctcga ccgatgaata taatccttac ctggtcaaga tggctgatgg ttacctggtg
     3481 ctggtctttg gttcagaccg cgcttgtgcc ggatgttcgg ccgggacgca taatctattt
     3541 cttgcgcgat ctgtttctgt ttataacgac gacgcaaagc tgccgacatt taacgcacct
     3601 gtggcattta cggtggccag caacccggtc aacgacggtg gcaggataag tttcgccgca
     3661 gtcagaaact ctgcgcagct acgcatctat ctcatgagca gtggtatcat caagtacgcg
     3721 gatgtgaata cagcaggcgc tccgtacaac gtagctgcac tcacctcgat agcgcacttt
     3781 ggcctgtcgc gcaccacgct gctcggtatc gacgctacgg gtagcaaatt ggtcgcgcgt
     3841 gcttccggcg cggcgcacac gtagctcaac acaaccttcg ccacggcaaa agtaacgccg
     3901 cggttcatga ctgtccttta cgccggcagt aaagagggca cgctggttgc gctttcggcg
     3961 gcaggtgccg gaggtacact cgacgatctc tacatacttg aaggcaccag tgccggtatg
     4021 ctgtgggaac agcagtcctc gaaaccccaa tcaggtgcag caacagcacc agtgtgggtg
     4081 caaccgcagc taacagccac cagagtctac gggcaaaacg gtttgttcac ctgcgccgtt
     4141 gttaataata ataattcagg ctgcacatcg ggtggcggga cgccaaatga caaaagcctg
     4201 taccgaggca gtcggcctgc gatctctgac gacgggctct acatcgccga ccagttgaac
     4261 aaccgcgccc tgtttttttc tggcatttcc acaactgcta cccgtgtcta ctgccaggca
     4321 ggtagtttca ccaccgcaac attcggcacc gttccgggtt cgcagacatg ccgtggaaac
     4381 ccgtcatatg gcaacgtctc ttcggtattt gtggattctt ctggcgtgta tgtgaatgac
     4441 attgaagcaa accgcgtgct gttttttgcc ggtgcgaata tcgatgccgc cagcggtcta
     4501 ggcggccgta tctatggcca aggcggcaat tataccacat tcgccgctgg cacgaccgcg
     4561 cagactttta ccaatcctga cacgacaacc tctgatattg acggcatcta cattgcagac
     4621 aacggcaata atcgtgtcct gttctatcca ggtacatcca ccacggctac ccgtgtctac
     4681 ggacaatcca acatgacctc aaacaccgca gggggcgggg cgagcagtct caactggcca
     4741 tggggagtta aatattttgg cggcggtatc tacgtggtcg actacaacaa ccaccgcgta
     4801 ctgttctttc ccgaaaactc gggtactgcc acgcacgtct ggggccaaaa cggcagcttc
     4861 accgcgaact ccgccaacca gggtggaagc gtctcggcaa cctcgctcaa taacccgtac
     4921 gcagtggacg tcgatcacac cggcatgtac gttggcgata accagaacca ccgggtggtt
     4981 ttctatccgc gctagggtca caggcgagcc cacggtgctc cgcaaagtct ggcaatagtg
     5041 tggtgcacaa attagtgaca agtgcgtcat tttttgcaac cgcctcaccg gcgtagtctt
     5101 ctaattaccg atgaaacttg cttttaagcg gcccccggtg ggccgaaccc aatgggtatc
     5161 gatcgcgttt cttagcctat ctccggtcat tggctttggg gcgctcgcgt attttctgca
     5221 taccggaact ttgtcgtctg cgacagtcac gctcgcagtg gttattgcga ccatcgcggg
     5281 catgggcaca accgcaggct accaccgcct gttctcgcac cgctcatacg aagcggcttt
     5341 gcccgtgcgt gtcttcatgc tgctgatcgg gctgctcggc ctgcagggtt cggcgctcga
     5401 atggtcgctc gaccaccgca cgcaccacaa gtttgtcgac cgcgacaaag atccgtacag
     5461 catcacgaaa ggtttctggt ttgcccacat gctctggatt ttcaaaaccc gcccccgcga
     5521 ctggcggccg tcgcacaacg ccgacctctt tcgtgacaag tttgttttct ggcagcaccg
     5581 ctttttcatg ccgctcgcag tggttgccaa ctttatttta ccggctgctg ttgccggtct
     5641 tttctggggc gacctttggg ggggattgct cattgcggga ttcttaagac tcgtcttcac
     5701 gcatcactcc acattcttca tcaattcact ttgccatatt gcgggttcac agccttacag
     5761 caacaagcat acagcgcgcg acaactggat caccgcgttc tttacgtggg gcgaaggtta
     5821 ccataacttt caccacgagt ttgcgaacga ctaccgcaac ggtgtgcgct ggtaccagta
     5881 tgacccgggc aaatggctga tctggctgct gtcacacctt catctcgcct cgaatctcaa
     5941 gcgggttccc gacaccacca tcaggcagcg ttgcatgcag atgcagaaag gcgagctgct
     6001 ctcgaaactg gaaaaatggg gatggaccga agaacgcaag cagctgtttc gtgcgcagct
     6061 cgaagagacg tttgccgctt tcaggcgccg tgccacggtg atgaatgaaa tccgcaaccg
     6121 aatgaaaagt gatgcggtac aggcaacgca gcgtcgcttt gagctaatgc aggagcgcta
     6181 tgagcgcctg caggatgcgg tagaagaaac ggccgtgcag tggaagttgc tgcgccgccg
     6241 tgcgcgtatt taaaggggct gtataaagat cacgcacggc cagtggccgt gcgcgtgcct
     6301 agctttttac gaaagccgcg atcttggtga gcgcggcttc aatttctttg cgcgcagttg
     6361 cgtacgagca gcggataaaa ccttcgcccg aatcgccaaa ggcagtaccg ggtacgacag
     6421 ccacgttctg cgcttcgagc agctcggtgg cgaattgcat cgagttcttg ccggtcgagc
     6481 ggatatcggg gaaaacgtag aatgcgccct gcggcatgaa gcacttgagt cccatgtcgt
     6541 tgaagccctt gacgatcagg tcacggcgca ggcggtattc atcgcgcatt ctgtcgcggt
     6601 cggcgagtgc atacttgagc gctgtctcag ccgcaatctg ccccagcgtg ggggcacaca
     6661 gcatcgaata ctggtgaatc ttggtcatgg atttgatgag gttctcgtcg ccgcaggcat
     6721 aacctatgcg ccagccggtc atggcgaaag ccttcgagaa gcccccgagc agcagtgtgc
     6781 gctctttcat gccgggtaag gtcgggaaag gcacatgctc cccttcatag ttgagttcac
     6841 cgtagatctc gtctgaaatg acgatcaggt tcttctcttc tgccaggcgc gcaatgcgcc
     6901 tgagctcgtc tgcaccgata gaagcaccgg tgggattcga ggggtaattg aggaacagga
     6961 ccttgctttg cacaccgccc gcatgcacga caccgctgat attctcagcc ttgagggaaa
     7021 aatcattctc ggccgtcgcc agcaccttct gcggaatgcc accggcaagg cggatcatgg
     7081 gatcatacga aacgtaacac ggttcggggt agagcacacc gtcgccgggg ttgagcagcg
     7141 cgcggaatgc aaggtccaca ccctgcgaaa cccccaccgt gatcaggatt tctttttccg
     7201 ggttgtagga gaggttatac tctttttcga gataacgcgc gatttcgcgg cgcagcgaga
     7261 gaaacccgga attaccggtg tagtgggtgt agccatcgcg cagagcctgt atcgaatgat
     7321 cgatgacaac ctgcggcgtg acaaaatcgg gttcacctac gcccagcgag atacagtctt
     7381 tgcgtgtcgc aacgatgtca aaaaactgcc tgatacctga aggcttcagg gtttgcacca
     7441 cggaagaaag tttttcagac cattcgatca tggaaattga cgcgccgcct gcctcagcct
     7501 ggcaaagggc aatcggtcta cttcagcagc gcctcgacgc cgggcagttt cttgccctcg
     7561 aggtattcga gcgtggcacc accaccggtc gaaacgtgag tcatcttgtc ttcgaggcct
     7621 gactgcgtca gggcaaagat cgaatctcca ccgccgacaa tcgtcgaacc tttcaccttc
     7681 gccatcgctt tggctatggc aaaggtgccg ccggagaact ttttcatttc gaaaacaccc
     7741 atcggtccgt tccagagaac agttttggcg ttcttgataa cttttgcgta gtggtcggct
     7801 gttttggggc cgatgtccat gcccatgaag ccgtcgggta tcgacttctt cactgtttta
     7861 atcttacctt tctcgccgaa ctcagaagag acgatgtggt cttctggcag cagcaattca
     7921 cgcttgttat aagcagcctt gtcaataatc tggaatgcct ggctcaggta gtccttttca
     7981 atcatcgacg tgccgacctc aatcagacgc gatttcagga acgtataagc catcgctccc
     8041 ccgatcaaaa tactgtcggc cttggtgacc aggttctcga gtaccgcgag tttgctcgaa
     8101 acctttgaac caccgatgat tgccacaaac gggcggggtg ggtcggtcac cagtttctgc
     8161 aggatatcaa tttctttctt cagcagcaga cctgcaaaac ccggcagcaa tttggcgcac
     8221 tcatagatcg aggcatgcgc acggtgtgcg gcgccgaagg catcgttcac gtagatgtca
     8281 gccagtttgc agagcttctt ggcaaagtca ttgcggactt tctcttcttt tgaagtctct
     8341 tcgtcgtaat aacggatatt ctcgagtacc aggatttcgc ccgccttgag cttacctgcg
     8401 agttcttctg cgaccggacc gaccgcttcg ctggcaaagt tcactgtacg gcccggcagc
     8461 aaggtgccta ggtgggctgc aatgttcttg agcgaaagtg ccgggtcgcc tttcttttcg
     8521 gggcgtccca ggtgtgaaat gatgataact gatgccccgc ggttcaacag cagttcgatg
     8581 cttggcaggc tcttcaccat gcgggtgtcg tcgctgatcg cgccggtcgc cttgtcgagg
     8641 gggacgttaa aatcgacgcg caggaggact tttttgccgc tgacttcggc ctgttcaatt
     8701 gagggcaatc ccatgcagct gaaacctaga ttttctccgc ggggtaaatc atttatgtgc
     8761 ccgcccgacg aggcccaggc cgggtttttt tgctctgccc gggaccccaa aatggccgat
     8821 tctagaaaga gtaatggctg aaacgcagga caagggcagg tcacgcaagg cgctggggct
     8881 gctctttgaa caggcgctcg aggccgaaaa acggggcgag ttgcgcaccg cactggagaa
     8941 atacaagatg gctctgcaaa ttgaccagaa cttcttcgat gcctggctga atgccggcgc
     9001 aatctacacc cgccagggac gctccgacaa ggcgattgtc tgctaccagc gggcgctcgt
     9061 ttcgaagaac gacgtgcggg cacactacaa ccttgcggtg gagttctata agcgcgagga
     9121 tttcgccggt gcagagaagg cgctccgatc cgttctcaaa ctcgactcgc gttacctcaa
     9181 tgcgcacctt ttgctgggct acgtgttcgg caagaccggg cgcaatgaca agtcagagat
     9241 ttcgatccgc aatgcgctca agatcgaccc gcagaatgtg caggcacaga cagcgctcgc
     9301 attgctgtat tttcacaccg acaggcatga gctggcccag cggcagatac agctgctgtt
     9361 gaataagagt cccgaagata aggttctgct
//
LOCUS       DAOQJF010000100         9358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3899_length_9358_cov_4.602709, whole genome shotgun sequence.
ACCESSION   DAOQJF010000100 DAOQJF010000000
VERSION     DAOQJF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3899_length_9358_cov_4.602709"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3298
                     /locus_tag="PLF85_06980"
     CDS             <1..3298
                     /locus_tag="PLF85_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19443.1"
                     /translation="WGVTTGKRACGSKTCNGRWNVSGLNMGANGDGYVPNSGNGFIAE
                     TPAGAQPGVTVNRTVRLGAQSYTAGDSMVLMEISFRQWGLTGLLVDVYDQIWASLGDG
                     PVTPAMIKDNFDSVLTLANALYTSSDILSGGFNGTELNIAKFRQFYDSFFPTDETTCT
                     GGVLSNGANAIPDLYENYLNGCGVTARELPPVPKLNPDPNNCNEFVQTACVRYPNAYN
                     ATTGAVTGWYAYTGPSDGKLTGMLTPLVVLFGTMHEDGAVIKVTGQGTDSLSGVYSAV
                     KYTGSPMVAGDNRDNVTIRNFCSNGAPGCTTANLGYRVELETLFSMLASLNETKKNNA
                     NNLPQYETTALTNILTESAAGLRNGLMPKLTNNKYANVAYIDPLVRDLESMISDNVRK
                     AEDNFKITSGTEVSASGMKNRDRLRYFATKNAVNPEVVVEKVVIANPGIVQKKYHGLE
                     AGDKVFITTTGTLPTELTAGTATAGPYYFVKAPVTSDTFRLSSTNGGAALQFSGAASG
                     THKIHPVVFRTNRFDQSVNGTALNGVPINMMKKFVKYLRSLASDTEVVAAIKAAIPML
                     NNYLNYVQNSTTQITLTDEDIDHIVDFIRDTNSSGDPSVDSFLDMLVATKMDDLNTLR
                     SFNFDQFKTLGSYQSVFDDINDKVNKYFEINIKKDLLYSPFMLGEPTCPGGSQNGFYD
                     HNENGVWDPGTFTFVSAAYTGASSWTDTANVGGSCTITTQIPNIYDKDYYMFDMGGVA
                     RNLQKAVNDITVSDVDQTLDWLYGRTLLGSPSDVVYAANPNNGNKVECHITSTGSGGG
                     INFDNEIRAFKNLLLCEVYNKKLEEPHYDRNGNNIIEAGEYTDINNNGFYDDKASASP
                     VMMRRMINYYLEEVFMPKYAEFIQPDPLSPNKNYVSFGANALNDLLSPTVCDANGLNC
                     GVNANYVTADLLQARSTFYASTNFTSSELKSVKNVIGNFLYDSETNTYTELLARTGPH
                     IVTVLQEFQGDYNDLLNMGLEGFKSDGFMTYFSTNLTNKSPYTSLDVLGDVRTLFNTQ
                     TMRCYPGITGEYAGGPQYCKKYKALDTFWGQFGLLMDQFSSAAYHKYKNQWQSQMNST
                     YYARLVSIFE"
     gene            3305..4852
                     /locus_tag="PLF85_06985"
     CDS             3305..4852
                     /locus_tag="PLF85_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19444.1"
                     /translation="MKFNEIIRGMRGIPAALLLLSLAPQVLRADIAETFGMGPRMIGT
                     SGAGVANINDWTASFYNMAGVASPMSDRAIFGNQEKSEEASGKIKLLKDDGSLQSDEK
                     GNKDAIDKKLESEVLTRDDKPAHQMGMNYLFQATLPTLSPKAMGPETAKNVDLAKKNM
                     VYGAIQMGLVFDTRSVINTPKNIPIRVGVSLSVPQSAVATVNDTKIESYNFMRYGREA
                     QRMLIIAGVGAQVWRNRLSIGVGANTFTGGRGKFEMKNVDIDPTGNTQVPNAETQMDL
                     TPTVAPVAGVQYRHNIKNRILLVGASWRGEIMMQLDPLDANATTQLLQVGLPLRLAIL
                     DFYSPHTFTLGFTYLHDEALKLSLDGELQLWNRFQVNSARAAYMQKAGEYFERFRNII
                     LVRFGAETRPGRYIRKLSDVPLFVRTGFSYTPAFTPDQTGYSNFLDNDKIGYSLGASY
                     FLNANSIVKVPVELIFGFQHQIMLTRTSSKSGDVTSSQSYISGNQPDYTYGGHVIVAT
                     LGALMKF"
     gene            complement(4880..6178)
                     /locus_tag="PLF85_06990"
     CDS             complement(4880..6178)
                     /locus_tag="PLF85_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNE19445.1"
                     /translation="MGGAFRSGGSNWFYVGREKKLQLFDGALQARQLVQLAPDTAAID
                     FADIDKDGDDDLIEFSHTGVTVRRWRKSDFAAPQQIIRERLALPIYVDNLEQSRLTVD
                     FDADGYTDFFLPAEGKFLVYRNESGTKFTRTIVLPYQPRGSFTNRLWQNSDLPSNSIR
                     STVIIPQPFFLDFNRDGILDAAARIDDRVYYFLSTTKNGAQQAFAETILRIYPMPQED
                     IYVAYAEFADFDGDGNLDLIYSAVKGLGLNIRADLRIFQGKSFIPDPAAMTEHSVKGG
                     VFSPLLADLKGRKLLILPTIDTGIGFFMNYILRSKVSLTLQFLDPLAKQGNPLEKTTL
                     NFDSKESAVPGFAYGDFNGDGQTDFILGTEISGITVYGGNPNLAKKEIAQIEAPAFGI
                     FKPVSNKDGSFNLFIYMTQRSKADKKTAVYLAPIRAKPLP"
     gene            complement(6301..7047)
                     /gene="fabG"
                     /locus_tag="PLF85_06995"
     CDS             complement(6301..7047)
                     /gene="fabG"
                     /locus_tag="PLF85_06995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNE19446.1"
                     /translation="MFKDRVAVITGSARGIGRAIAEALAEQGCNIVISDVNAEGCQKT
                     ADEIAAKYGVKAIGQAANVTKKEDMTALANAAVEKLGKLDIWVNNAGVLRDDLLMRMS
                     EEDWNLVLDVNLKGVFFGIQAATKVMMKAKYGKIVNISSVAGLIGNAGQANYSAAKAG
                     VIAITKTAAREYASRNININAITPGYIQTDMTGALPQKVQEELAKMVPLPNKGAPSDI
                     AKAARFLASSDADFITGVILRVDGGMAIGF"
     gene            7325..8962
                     /locus_tag="PLF85_07000"
     CDS             7325..8962
                     /locus_tag="PLF85_07000"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNE19447.1"
                     /translation="MKKARYIFVTGGVASSLGKGVSIASIGALLEARGFKVTIQKLDP
                     YINIDPGTMSPFQHGEVYVTEDGAETDLDLGYYERFTNASLGRENSVSTGQIYHSVIS
                     RERRGEYLGRTVQVIPHITNEIKNRILLLGQMNPDLDFILVEIGGTVGDIESVPFLEA
                     IRQFRLDYGQENTMFIHLTLVPSITKGGEIKTKPTQHSVKELQKQGIQPDILLCRTTT
                     SLDAEVKEKISLFCNVSPRRVFSAPDIDHTIYEIPQIFHSENLDEEIVGYFRMESKSG
                     EYLMNLNPMLAKWEQVVDVFRSPRHHVKIAVVGKYMALHDAYRSLYEALTHGGFPRKI
                     AVDFIKIDSEKVEPENFRELLKDAQGILVPGGFGERGVEGKLLAIRYARESKIPYFGI
                     CLGMQCAVIEFARNVLGYKDAHSTEFAPDSAYPVISLMEEQTAVVNKGGTMRLGAYPC
                     KLREGSLIAECYMQPMIQERHRHRFEFNNKYRNAFESAGLRLTGLSPDESLVEAVELD
                     RSVHPFFVGTQYHPEFKSKPLAPHPLFTRFIEKSAGL"
     gene            8962..>9358
                     /locus_tag="PLF85_07005"
     CDS             8962..>9358
                     /locus_tag="PLF85_07005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE19448.1"
                     /translation="MLFDNFSAVPEEVALLNAEMAAMGDKSPVALEIPPKCFFDMQGK
                     FLRYRSRKAILVSCPVLEKQLNPIGVMQGGYMAAAFDNTLGPLSYAAAQKAAVTLDMS
                     QTYLRAARAGETIWCAAEVVTRSPRTLYMT"
BASE COUNT         2225 a         2420 c         2522 g         2191 t
ORIGIN      
        1 ttggggcgtt acaacgggga aacgtgcctg tggttcaaaa acctgcaacg gtcgctggaa
       61 tgtttccggt cttaatatgg gagccaacgg cgatggttat gttccgaaca gcggtaatgg
      121 ttttatagca gagactcctg ctggtgcaca gcccggagtg actgtgaatc gaaccgttcg
      181 ccttggtgcg caatcttaca ctgccggcga ttcgatggtt cttatggaga tatcctttcg
      241 gcagtgggga ttgacgggcc tcctggtaga tgtctatgac cagatctggg ccagccttgg
      301 cgatggccct gtaactccgg cgatgatcaa agacaacttc gattcggtac tcactctcgc
      361 caacgccctc tatacctctt ctgatatcct cagcggtggc tttaacggta cagagctgaa
      421 tatcgcgaaa ttccgccagt tctatgattc ctttttcccc accgacgaaa ccacctgcac
      481 aggtggtgtg ctttctaacg gagccaatgc gataccagac ctgtatgaga attatctgaa
      541 cggttgcggt gtgaccgccc gggaattgcc accagttcct aagctgaacc cagacccgaa
      601 caactgtaac gaattcgtgc agacggcctg cgtgcgttat cctaacgcat acaacgcgac
      661 taccggggcc gtgactgggt ggtatgcgta cacaggacca tcagacggca aactgaccgg
      721 tatgctcaca ccactcgtgg tactgttcgg gacaatgcat gaagatggtg ctgtcatcaa
      781 ggtcacaggg cagggaacgg attcgttatc gggggtgtac agcgccgtga agtatacggg
      841 ttcgcccatg gtcgcgggtg acaatcggga caatgtcacc attcgtaact tttgttccaa
      901 cggggcgccg gggtgcacca ccgcgaacct gggttaccgg gtagagctcg agacactctt
      961 ctctatgctc gcttcgctca atgaaacgaa aaagaataac gctaacaacc tgccgcagta
     1021 cgaaaccacc gcgctgacca acatactgac cgaatcagct gccgggctgc gtaacggcct
     1081 catgccaaaa ctgaccaata acaagtatgc gaatgttgct tatatcgatc cgctggtgcg
     1141 cgatctcgag agtatgatct ctgataacgt gcgcaaggca gaggacaact tcaagatcac
     1201 ttccgggaca gaagtatcgg cttcgggcat gaaaaaccgc gatcgcctcc gctatttcgc
     1261 gaccaagaat gccgtgaacc cggaagttgt ggttgaaaag gtcgtcatcg cgaatccggg
     1321 tatcgtgcag aaaaagtacc acgggcttga ggcgggtgat aaggtattca ttaccacgac
     1381 gggcacattg ccgacggagc ttacagctgg tacggcaaca gctggaccat actattttgt
     1441 caaggcaccg gtaacctcag ataccttcag gctgagctca acgaatggcg gtgcagcgct
     1501 gcagtttagc ggagctgctt ccggaacgca caagattcac ccggttgtat tcaggacaaa
     1561 ccgttttgac cagagtgtca atggtaccgc tctgaacgga gtgcccatca acatgatgaa
     1621 gaaattcgtg aaatacctgc gttcgctggc atctgacacc gaggttgtcg ccgcaattaa
     1681 agcggcaata ccgatgctca acaactacct gaactacgtc cagaacagta ccacgcagat
     1741 cacgctcacc gatgaagata tcgaccatat cgttgatttt atccgcgaca cgaacagcag
     1801 cggcgatccg agtgttgata gctttctcga catgcttgtt gcgaccaaaa tggatgacct
     1861 gaatacactg cgttcattca attttgacca gttcaaaaca ttgggctcgt accagagcgt
     1921 tttcgatgat atcaacgaca aggtgaacaa gtactttgag atcaacatca agaaagacct
     1981 gctctacagc ccgttcatgc tgggtgagcc aacatgtccg ggtggttcgc agaacggatt
     2041 ctatgaccac aacgaaaatg gtgtctggga tccaggcaca ttcacgtttg tttcagcagc
     2101 ctacacaggt gcttcttcgt ggacagatac ggcgaacgta gggggtagtt gtaccattac
     2161 gacgcaaata cccaacattt acgataaaga ctactacatg tttgacatgg gtggggtagc
     2221 acgtaacctg cagaaggcgg tcaacgatat caccgtctca gacgttgacc agacgctcga
     2281 ttggctttac ggtcgcaccc tgctaggttc gccgagcgac gttgtctatg cggctaaccc
     2341 gaataatggc aataaggttg aatgccacat tacctcaacc gggtcagggg gggggattaa
     2401 ctttgacaat gagataagag cattcaaaaa cctcttactc tgcgaagtct ataacaagaa
     2461 gctcgaagaa ccgcactacg accgcaacgg caataatatc atagaagccg gtgaatatac
     2521 cgatatcaac aacaacggtt tttacgatga taaggcttct gcatccccgg tgatgatgcg
     2581 acgcatgatt aactactacc tggaagaagt tttcatgccc aagtatgctg agttcataca
     2641 gcccgatccg cttagcccga acaagaatta tgtgagtttt ggtgccaacg cgctgaacga
     2701 tcttctttca ccgactgtct gtgatgccaa tggtctcaat tgcggggtaa atgcgaacta
     2761 cgtgacggcg gatcttctgc aggcacgctc taccttctat gcgagcacca acttcacgtc
     2821 atcggaacta aagtcagtaa agaacgtgat tggaaacttc ctctacgaca gtgaaacgaa
     2881 tacgtacact gagctgttgg cgcgcacggg gccgcatatc gtaacagtac tgcaggaatt
     2941 ccagggtgat tacaatgacc tgctcaacat ggggcttgaa ggctttaaga gtgatggttt
     3001 catgacctat ttttcgacaa acctgaccaa taagtcgccg tatacttcgc tcgacgtgct
     3061 gggtgacgta cgcacactgt tcaacacaca gaccatgcgg tgttacccgg gcattactgg
     3121 cgagtatgcc ggtggtccgc agtactgcaa gaaatacaag gccctggata ctttctgggg
     3181 gcagtttgga ctgctgatgg accagttctc gagcgcggcc tatcataaat acaaaaacca
     3241 gtggcagtca cagatgaatt cgacctatta cgcgcgcctg gtatcaatat tcgagtgagg
     3301 ttgaatgaag tttaacgaaa ttatacgcgg aatgcgcggc attcccgcag ccctgttatt
     3361 actgtcgctc gcaccccagg ttttgagggc tgatatcgct gaaacttttg gcatgggacc
     3421 cagaatgatc ggcacctcgg gcgcgggtgt ggcaaacatc aacgactgga ccgcgagttt
     3481 ctacaacatg gcgggcgttg cctcgccgat gagcgaccgg gcgatttttg gcaaccagga
     3541 gaagtctgaa gaggcgtcgg gtaagatcaa actgctcaag gatgatggtt cgctgcaaag
     3601 cgacgagaag ggcaataaag acgcgatcga caaaaaactt gaatcagagg tcctgacgcg
     3661 tgacgacaaa ccggcgcacc agatgggtat gaactacctg tttcaggcga cgctgcccac
     3721 actatccccc aaggccatgg ggcctgagac tgcgaagaac gtcgacttgg ccaagaagaa
     3781 catggtctac ggcgcaatac agatgggatt agtttttgat acccggtcgg ttattaacac
     3841 gcctaagaat attcccattc gtgtcggcgt ttcactttcc gtgccgcagt ccgcggtggc
     3901 gaccgtcaac gatacgaaaa ttgaatcgta taatttcatg cgatatggcc gcgaagccca
     3961 gcggatgttg attatcgctg gcgtcggtgc gcaggtctgg cgtaaccggt tgagtatcgg
     4021 agtaggtgct aacaccttta ccggcgggcg cggtaagttt gaaatgaaga acgtagacat
     4081 tgatcccact ggtaacacgc aggtacctaa tgctgaaacg caaatggacc taaccccaac
     4141 agttgcgcct gtagcgggag tgcagtaccg gcacaatatc aagaatcgta ttctcctggt
     4201 cggggcatcc tggcgcgggg aaatcatgat gcagctggat ccgctcgatg ccaatgctac
     4261 tacccagctg cttcaggtgg gattaccgct ccggctggcg attctcgact tctactcgcc
     4321 gcatacattt acccttggct ttacctacct gcacgacgag gcgctcaagc tgtcgctcga
     4381 cggcgaactg cagctgtgga accgtttcca ggtgaatagc gcgcgggctg catacatgca
     4441 gaaggcgggt gaatattttg agcgcttccg gaatatcatt ctcgtcaggt ttggcgccga
     4501 aacgcgcccg gggcgttata tcaggaaact cagcgacgtt ccgctcttcg tgcgcacagg
     4561 attttcttac actcccgcct ttactccgga ccagacggga tattcaaact ttctcgacaa
     4621 tgacaagatt ggttattcac tgggagcttc gtacttcctg aatgccaact cgatcgtgaa
     4681 agtaccggtg gagctcatat tcggtttcca gcaccaaatc atgttgactc gtacgagcag
     4741 caagtcagga gatgtgactt catcgcagtc gtatatatcg ggcaatcaac ccgactatac
     4801 ttacggaggt catgtgatcg tggccacgtt gggcgccctc atgaagttct gagtggttcc
     4861 agcgccgttg ccgtttaact cacggcaacg gtttcgccct gatcggggcg aggtacacag
     4921 ctgtcttctt gtctgctttt gaccgctgcg tcatgtaaat aaacaggtta aacgaaccgt
     4981 ctttgttgct cacaggtttg aagataccga atgccggtgc ttctatttgc gcgatttctt
     5041 tctttgcgag attcgggttg ccaccgtaga cggtgatgcc actgatttcg gtgcccagta
     5101 tgaagtcggt ctgtccatcg ccgttgaagt cgccgtacgc gaagccgggt accgcagatt
     5161 ctttcgagtc aaaattcaat gtcgtctttt caagaggatt gccctgtttc gccaacgggt
     5221 caaggaactg cagtgtcagc gaaacctttg accgcaggat ataattcatg aagaacccga
     5281 tacctgtatc aatcgttggt aaaataagga gctttctccc tttcaggtca gccaacagcg
     5341 gagaaaaaac tcccccttta actgagtgtt cggtcatggc cgctggatcg ggtatgaagg
     5401 atttaccctg gaagatcctg agatcagcgc ggatattcag tcccagccct ttcaccgcag
     5461 agtagatcag gtccaggtta ccatcgccat cgaagtcggc gaattcggca tacgcgacat
     5521 agatatcttc ctgcggcatc gggtagatgc gcagtatagt ttcggcaaag gcctgttgcg
     5581 cgccattctt cgtggtgcta agaaaatagt acaccctgtc gtcgatgcgc gcagcagcat
     5641 cgagaatgcc gtcgcggtta aaatccagaa agaagggttg tggaataata acagtagagc
     5701 gtatactatt agagggcaga tcggagtttt gccagagtcg gttggtaaaa ctgccgcggg
     5761 gctggtaggg caggacaatg gtccgcgtga attttgttcc ggattcattg cggtagacga
     5821 ggaatttgcc ttcagccggc aggaagaagt ccgtgtaacc gtcggcgtca aagtcgacgg
     5881 tcagccggct ctgctcgagg ttgtctacgt agatgggcag cgcaagccgc tcacgtatga
     5941 tctgttgtgg cgcggcgaaa tcagattttc tccagcgtcg gactgttacc cccgtgtgcg
     6001 agaactcgat caggtcgtcg tcgccatctt tatcgatgtc ggcaaagtcg attgcggccg
     6061 tgtcgggagc tagttgcaca agctgcctgg cttgaagtgc gccgtcgaag agctgcaatt
     6121 tcttctcgcg tccgacatag aaccagttcg atccacccga acgaaaggcg ccacccattt
     6181 cgggaatgaa acccgcctcg gcggtacgaa cgccattggc ttgcgctgat agaaatgctg
     6241 aaaaaagaaa aaaggccgca caaccggcgg cctttatcgg aatcaaatgt gggttcagga
     6301 tcagaagccg attgccatac cgccgtcaac ccgcaggatc acgccggtaa tgaagtctgc
     6361 gtcagacgac gcgaggaagc gtgcagcttt cgcgatatca gaaggagctc ccttgttggg
     6421 cagtggtacc atcttggcga gttcttcctg gactttttgt ggcagagcgc cagtcatatc
     6481 agtctggata tagccaggcg tgatcgcgtt aatgttgatg ttgcgtgacg cgtattcgcg
     6541 cgctgcagtt tttgtaatgg caattactcc ggcttttgcc gcagagtagt tcgcctggcc
     6601 cgcgttaccg ataaggccag ctacagatga gatattcacg atcttgccgt atttcgcctt
     6661 catcatcacc ttggttgcag cctgaatacc gaagaacact cccttgaggt tcacgtcgag
     6721 caccaggttc cagtcttctt ctgacatgcg catcagcagg tcgtcgcgca ggacgccggc
     6781 attgttcacc cagatgtcga gtttgcccag cttttccact gctgcgttgg ccagtgctgt
     6841 catatcttct tttttggtga cgtttgccgc ctggccgatc gccttcacgc catatttggc
     6901 agcgatttcg tcggcagtct tctggcaacc ttcggcattg acgtcagaaa tgacgatgtt
     6961 gcatccttgt tcggcaagcg cttctgcgat ggcacggccg atgccccgcg ctgaacccgt
     7021 aattacggcc actctgtctt taaacatgac ggagtgttcg gtaattttac tcagtctgac
     7081 aagttttttg gttgtggagc caacgggtct gcagtgctat gggctgcaca gcaggtctca
     7141 aatcaggaat tgcggccgaa agttccgcat ttttcagatg aaaaatgctc catgaaccgg
     7201 ccaagtggcc tgctttcgac cgcaattcat gatttgatgg gaccttcctg gatgatctat
     7261 ttacgacctg cagcacagcc cctatggttg acggtgcatt tcagaacgcg acataatccg
     7321 gctcatgaag aaagcgcggt acatctttgt gacgggtggg gtggcaagtt cactcggcaa
     7381 gggagtatcc attgcttcta taggtgcgct gctcgaggcg cgcggtttca aggtcaccat
     7441 ccagaaactc gacccctaca tcaatattga tccaggcacc atgagcccgt tccagcacgg
     7501 cgaagtgtat gtcaccgaag acggggctga aaccgattta gacctcggtt attacgagcg
     7561 ctttaccaat gcgagcctgg gtcgcgaaaa ttccgtttct accggccaga tatatcacag
     7621 cgtcatttcg cgtgagcgcc gcggcgagta cctcgggcgc accgtgcagg tgattccgca
     7681 tattaccaat gagatcaaga accgtattct gctgctaggg cagatgaatc ccgacctgga
     7741 ttttattttg gtggagatcg gtggtaccgt cggtgacatc gagtcggtac cctttctcga
     7801 agcgatcagg cagtttcgcc ttgactatgg ccaggaaaac acgatgttca ttcatctcac
     7861 ccttgtgcca tccatcacga aaggcgggga gatcaagacc aaaccgacac agcactctgt
     7921 gaaggagcta cagaagcagg gaattcagcc cgacatttta ctctgccgca ccacgacttc
     7981 gctcgatgca gaagttaaag aaaagatttc actgttctgt aacgtctcgc cgcgccgtgt
     8041 attctcggcc ccggatatag accacaccat ttacgaaatt cctcagattt ttcacagcga
     8101 aaacctcgat gaagaaattg tcggttactt ccgcatggaa agcaagtcgg gcgagtacct
     8161 gatgaacctc aacccgatgc ttgccaagtg ggagcaggtg gttgatgtct tccgttcgcc
     8221 gcgacaccat gtgaagatcg cagtagtagg taaatatatg gctttgcacg acgcctaccg
     8281 ctcgctctat gaggcgctca cacacggtgg cttcccgcgc aaaattgccg tggattttat
     8341 taagatcgat tcagaaaagg tagagccaga gaactttcgc gaactgctga aagatgcgca
     8401 ggggatactc gtacccggcg gctttggcga gcgcggcgtc gaaggcaagt tgctggccat
     8461 acgctacgcg cgcgagtcaa agattcccta ctttggcatc tgcctcggca tgcagtgcgc
     8521 agtgattgaa tttgcgcgca atgtgctcgg ttataaagac gcgcactcga ccgagttcgc
     8581 tccggattcg gcctacccgg tgatcagcct gatggaagaa cagaccgctg tggtgaacaa
     8641 gggcggcacg atgcgcctcg gagcataccc gtgcaagttg cgcgaggggt cgctgattgc
     8701 tgagtgttat atgcagccaa tgatccagga aaggcaccgg caccgctttg aatttaacaa
     8761 caagtaccgc aatgcctttg aatcagccgg gctgaggctc accggccttt ctccggacga
     8821 gtcgctggtg gaagcggtgg agctcgatcg ttctgtgcat ccgttcttcg tggggacgca
     8881 gtaccatccg gaattcaaat ctaagccgct cgctccgcac ccgctcttta cgcgtttcat
     8941 cgaaaaaagc gcgggtctct gatgcttttc gataattttt ctgcggtccc ggaagaagtt
     9001 gcattgctca atgcggaaat ggcggcgatg ggcgataaat cgccggttgc gcttgaaatc
     9061 ccccccaaat gtttcttcga catgcagggg aagtttctgc gttaccgctc gcggaaggcc
     9121 attctggtga gctgtcccgt gctcgaaaag cagctgaacc cgattggggt catgcagggt
     9181 ggttatatgg ccgcggcttt cgacaatacg ctcggcccgc tgtcttacgc ggcggcgcag
     9241 aaagctgccg tgacgctcga catgtctcag acttacctgc gcgcagcgcg cgccggcgag
     9301 accatctggt gcgcagccga agtggtgacg cgttcgccgc gcacgctcta tatgacag
//
LOCUS       DAOQJF010000101         9324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3923_length_9324_cov_5.538138, whole genome shotgun sequence.
ACCESSION   DAOQJF010000101 DAOQJF010000000
VERSION     DAOQJF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3923_length_9324_cov_5.538138"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLF85_07010"
     CDS             complement(<1..720)
                     /locus_tag="PLF85_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE19449.1"
                     /translation="MKEILVIRFSAIGDVLLTTPVLQQLKAQFPEARIHFLVKEQYAE
                     FIAACPHQDNIITWKKEDTVFTVRQRLAARAPRFDLVLDLQSNLKSRVLSLLLPAGTK
                     RRYRKPYFNRLLLVYLKRNRYKDKKKISERYLEALEGIVEPPLTGRIELKRDKLPREV
                     TKFAQAPTILIAPGARWATKRWPEEYFVSLASKLLEKHATCHVVWLGGKDEEKTFSFF
                     AEHPYLRRHRKRMQFLPGILGV"
     gene            complement(717..1361)
                     /gene="tmk"
                     /locus_tag="PLF85_07015"
     CDS             complement(717..1361)
                     /gene="tmk"
                     /locus_tag="PLF85_07015"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNE19450.1"
                     /translation="MATGVFAVLEGIDGSGKSTTALELSRRLEERSLQTVVLREPTEK
                     TDASREIRRILRTVQSVDAAVSRELLDLFLIDRLWDLKHQIIPALKAGKIVLLDRYFL
                     STAAYQSANTTDVQAIMQSYLGDDRIRMPDILIHLTLPVEAALMRLEERRQLDVFETE
                     TRLNLIAHNYGTAITLLRESRPEVQVIELAHALGDEDFDLLADKIAAVAAKKKL"
     gene            1474..3384
                     /locus_tag="PLF85_07020"
     CDS             1474..3384
                     /locus_tag="PLF85_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE19451.1"
                     /translation="MAILNRYTMYEMVKTTAEKYSRLTAQLYRTDKNSPYTAVSVGDF
                     FDKARAISLGLDAIGLQRGDKVGLIADVGPQWLPISMGITAIGGVDVPRGTDATAQEL
                     LYIFKHADCPIIILENEKVYANISASLSEFANLKTIIFVNGAQPQVPGSIKVLGLDAV
                     IESGRAAHAANPGRYAELGSRVGESDLVTIIYTSGTTGNPKGVMHTHKSLCWEVWHVA
                     DALPITPGGVTMGFLPPWHVAERLIESVAFTRGVAIAFTSVATLAKDLQEARPTFLLS
                     VPRVWESFYNKVMDGVKKASPVARGLFNFARSSAAHYSIEKDILTNNKYRLDKPSVLF
                     HLLRRPLAVLNLLLLFVPNLLAQLILGKVRRGLGGRVQFALSGAGALPEHIDRFFYSI
                     GIAIVETYGMTETGGVTCRRTYPGTVIGTVGKPIGGTKVKLLDEQGNEVTKPNTKGVC
                     WHYGPHIMKGYYKEDQKTAEVLKDGWLNSGDILVYTANGELKFAGRAKDTIVLFGGEN
                     VEPQPIEDTLIQSEYIHQIVVVGQDRKTLGALIVPAKEAVLKYAEERKISLPAEIRDW
                     PLNAEIQKLYKTEIKDRVSEKAGFKNFEKVTTFAVIPDEFKIGEELTQTLKVRRNVVF
                     DKYAKQIDEMYK"
     gene            complement(3417..4388)
                     /locus_tag="PLF85_07025"
     CDS             complement(3417..4388)
                     /locus_tag="PLF85_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19452.1"
                     /translation="MPDRTLNDCFEETLRPLVTLKPLFLLRDGILNPFERVLLKHDRG
                     LRISSQRPAQELAQLRALHGLGHFDEAPANNPLQPADLLSRIGERITQDCRLLPEKYL
                     HPKDLQLEGSVEQLYVHPEAKVSRHAVIDSSEGPVVIDSQARISALSFVKGPAYVGRN
                     TQLDQCRFSNSIAGENCRLGGEIADSIIGDFSNKHHDGFLGHSLVGDWVNLGALTTTS
                     DLKNNYGEIRLHYSGKTYTTGTIKFGSIIGDYAKTAIGTLLGTGSIVGVGANLFGDSH
                     WSGDIAPFTWGRAGSYDRGLFIRDAGRMMQRRGQSVSAQLEGLLRTI"
     gene            complement(4388..5176)
                     /locus_tag="PLF85_07030"
     CDS             complement(4388..5176)
                     /locus_tag="PLF85_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichol kinase"
                     /protein_id="HNE19453.1"
                     /translation="MSSETKKLFSKDNKPGEKFNYARKLFHLLGLVIPVGYFFTWLDP
                     ISPWVFKETTRALIFWITGVITVFFLLIEFLRFRWQFWQDLFVKVAGPMLKGKEMDKM
                     HGSVPYILALCVVVGFFPRDIAILSVLFLTFGDPAAALVGGKYGTIRFYNGKSLQGTL
                     GGIGGAFLAGLIFLILISAAGVTSEYLLWDNHGIRLENWVMLFIGAIAALLIEFVSHE
                     GLLDDNLTIPVGASLLMIGTHSALSGHSLGSYFYSLFDLIQTIK"
     gene            complement(5180..5530)
                     /locus_tag="PLF85_07035"
     CDS             complement(5180..5530)
                     /locus_tag="PLF85_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE19454.1"
                     /translation="MTPAEQHFNVSEEKAQTYTFFKVKGAMNSQNTHEVRRRFLDAAT
                     RSHVLLDLSEVELIVSTGIGLLFEMSEQLSRGNKKLVLIQPSARVQQVISMTGFTEMF
                     SYAPNRLGAEKLLD"
     gene            5640..6860
                     /locus_tag="PLF85_07040"
     CDS             5640..6860
                     /locus_tag="PLF85_07040"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19455.1"
                     /translation="MLALVRSIIYAGADLDPHRRRYVINTNVYSLIVILISMPYPFVY
                     LYLKVDIEPILLSGSLIFVSSYVIVILLNYFKLHMLAKLLFFGTAVCQLFGICIVFGK
                     ETGLDQFFFAAFVAVFFVLDRHQKILIFPALAGFVAEYLLIQYILAYIDPVANVTGDS
                     ARIIRYITSGAVFAMLAAQCAHFYYSNLKGELEIEKARHQSDKLLLNILPEPIADELK
                     RTGSVEPVEHDSVTVVFTDFVGFTASSEFRNPSELLRELDDCFSAFDAIIEKYGLEKL
                     KTIGDAYMFAGGVPVNLEENAVRCVQAAQEIVAFVAGRRTHDPRFWQIRVGMHTGKLV
                     AGVIGRKKFIYDVCGDTVNTASRLEAAGEPGRINTSEETYNLINQTYRCEYRGEISVK
                     GKGALPMYFVGDPV"
     gene            complement(6881..7633)
                     /locus_tag="PLF85_07045"
     CDS             complement(6881..7633)
                     /locus_tag="PLF85_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE19456.1"
                     /translation="MAGHSKWKNIQHRKGAADAKRGALFTKLAKEIMVAAKLGGGDES
                     ANPRLRTAVLKARQNSMPKDNIDRAIKKGTGELEGVTYEEGVYEGYGPGGIAIMVEVL
                     TDKKSRTVPELKSTFKTNGGTMAEANAVAYLFDKKGLILVEGDNITEDDVMEIALDAG
                     AEDIEKDDEKVFAVKTAVNDFHAVLAAMEPKLTAKGYKIVESGLKYIPQTTIAVDGEK
                     GKEALELISALEDNDDVQNVYSNLELTESLMA"
     gene            complement(7691..>9324)
                     /locus_tag="PLF85_07050"
     CDS             complement(7691..>9324)
                     /locus_tag="PLF85_07050"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNE19457.1"
                     /translation="LYVYLPFGDMVHRKIEQIIREEMNRFGGVEVTLPVITPAELWQE
                     SGRWDVMGKEMMRMQDRHEVGYALGPTHEEAMTDLARSFLQSYKQLPINLYQIGRKYR
                     DEIRPRYGLIRCREFVMKDAYSFHVDDKSLDETYQKMREAYRAIFERCGLKTIPVEAD
                     SGNMGGSGSEEFMVASSIGEETLLLCSDEKCGYRSNQEKTAFLENTASGVTDRSPSAV
                     EAGIEDFETKELKTIDEVAKLTGEAPRNFIKAVIYEDNTTIVLCFIQGDRELSEVKMK
                     NLSGMAEYWLATEETIKTITGADPGYAGPVRLKYKDGDKVGKKVLRIFYDNHLRGRSG
                     LIAGANKTYFHTKNVAEGRDFTIAQGHDLINAHAGDICPQCKKAELTETRGIEVGHIF
                     KLGKKYSEKMGLSVLDPNGKPLTPTMGCYGIGVGRTMATSIEQNHDERGLVWHRNLSP
                     FLVYLVSLAKGEAETAYADKLYATLQAKGISVYLDDRDERAGVKFNDAELVGFPFIVT
                     VGKKTVESGKIELKLRQSGEKLEVTEAELLQKILS"
BASE COUNT         1999 a         2524 c         2590 g         2211 t
ORIGIN      
        1 cacgccgagt ataccaggca aaaactgcat gcgcttgcgg tggcggcgca ggtaagggtg
       61 ttcggcgaag aagctgaatg tcttttcttc atctttgccg ccgagccaga ccacgtggca
      121 cgtggcgtgt ttctcgagca gcttcgaggc aagcgaaaca aagtactctt cgggccagcg
      181 tttagttgcc cagcgcgccc ccggggcaat cagtatggtc ggggcctgtg cgaactttgt
      241 cacttcacgc ggcagcttgt cgcgcttcag ttcaatgcgt cctgtgagcg gcggttcgac
      301 gatcccctcg agcgcttcga gataccgttc gctgatcttc tttttatctt tatagcgatt
      361 gcgcttgagg taaacgagca gcagccggtt aaagtacggc ttgcggtaac ggcgttttgt
      421 accggcaggc aacagcagcg ataacacacg cgacttgagg ttgctttgca ggtcgagcac
      481 caggtcaaag cggggagctc ttgcggcgag gcgttgccgt accgtgaaaa ccgtatcttc
      541 ctttttccac gtgatgatgt tgtcctggtg agggcatgcg gcgataaatt ctgcgtactg
      601 ctctttgacc agaaaatgga tgcgagcttc aggaaactgg gccttgagct gctgcagaac
      661 cggcgttgtc agcaggacat cgccaatggc gctgaagcgt atgaccagga tttctttcat
      721 aatttctttt tcgccgcaac ggcggcaatt ttgtctgcca gtagatcaaa atcttcgtct
      781 cccaaggcat gggcaagttc aatgacctgc acctcgggac gtgattcacg caagagcgtg
      841 atcgccgtac cgtagttgtg agctatcagg ttaaggcggg tttcggtttc aaacacatca
      901 agctgccgcc gttcttcgag gcgcatcagt gcagcttcaa ccggcaacgt gaggtgtatg
      961 agaatatcgg gcatacgaat gcggtcatcg ccgaggtaag actgcatgat cgcctggaca
     1021 tctgtggtat tggctgactg gtaggcagcc gtggaaagaa aatagcggtc gagcagcaca
     1081 atcttaccgg ctttcagcgc gggaatgatc tggtgcttga gatcccacag gcgatcaatg
     1141 agaaagaggt cgagcagttc gcggctgact gcagcatcga cgctttgtac cgtgcggagt
     1201 atgcgcctga tttcgcggga ggcatcggtc ttttccgtcg gctcgcgcag aactactgtt
     1261 tgcagcgagc gttcttcgag ccggcgggaa agctcgaggg cagtcgtgct cttgccgctg
     1321 ccatctatac cttcaagaac cgcgaaaacg cctgttgcca tacaattgtg caggccactg
     1381 aaggcctgct ttttgtggca taagcaaaag tcttcctgag gcatacacca gtaaattaga
     1441 ttgcatctct gtgctgagtt cgcagaagtt cccatggcaa tactcaacag gtacacgatg
     1501 tacgaaatgg tgaaaaccac ggcggagaag tattcacgcc tcacggcgca actctaccgc
     1561 accgacaaga actcccccta tactgcggtc agcgtgggcg attttttcga caaggcgcgc
     1621 gccatcagcc ttggtctcga cgcgattggc ctgcagcggg gtgacaaggt gggtcttatc
     1681 gccgatgtcg ggccgcaatg gttgccgatt agcatgggta tcacggcaat cggcggtgtc
     1741 gatgtgcccc gtggtaccga tgcaacggcg caggagctat tgtatatttt taagcacgcc
     1801 gattgcccga tcatcattct cgagaatgaa aaagtttatg caaacatttc tgcctcgctc
     1861 agcgaattcg ccaacctcaa gacgatcatc ttcgtgaatg gtgcgcagcc gcaggtacct
     1921 ggctctatca aggtgctggg ccttgacgca gttattgaat ctggtcgtgc tgcgcacgcc
     1981 gcgaatcccg gtcgttatgc agagctcggc tcgcgcgtcg gcgaaagcga tctcgtgacg
     2041 attatctata cgtcgggtac gacgggaaat cccaagggcg tgatgcacac gcataagtca
     2101 ctttgctggg aagtctggca tgttgcagat gctctgccca tcacacccgg cggggttacc
     2161 atgggctttt taccgccctg gcacgtggcg gagcgcctga ttgagtcagt tgccttcacg
     2221 cgcggcgtgg caattgcctt cacctcggta gcaactctcg cgaaagacct gcaggaagcg
     2281 cgaccaacat tcctgctctc ggtaccccgc gtctgggaaa gtttctacaa caaggttatg
     2341 gacggcgtca aaaaggcatc gcccgttgcc cgcggcctgt ttaattttgc ccggtcgtcc
     2401 gccgcgcact attcgatcga gaaagatatt ctcacgaaca ataagtaccg cctcgacaaa
     2461 ccttcggtac tgtttcacct tctgcgccgg ccgctggctg tgctcaacct gcttctgctt
     2521 tttgtgccta acctgctcgc gcagctgata ctcggtaagg tgcgtcgcgg gcttggtggc
     2581 cgcgtgcagt ttgctctctc gggtgcaggc gcgctacccg agcacatcga caggtttttc
     2641 tattcgatcg gcatcgccat cgttgagacc tatggcatga ccgaaacggg tggcgtgaca
     2701 tgccggcgga cttatcccgg tactgtaatt ggcacggtgg gcaagccaat tggtggcaca
     2761 aaagtgaagc tgctcgacga acagggcaac gaagtgacca aacccaacac gaagggtgtg
     2821 tgctggcatt acggcccgca tatcatgaaa ggctattaca aagaagacca gaagacggcc
     2881 gaggtgctca aagatggctg gctgaactcg ggcgacatcc tggtctatac cgcgaacggc
     2941 gagctcaaat ttgccggccg tgcaaaagac accatcgtac tcttcggcgg agagaacgtc
     3001 gaaccgcagc cgatcgaaga cacgctgatc cagagtgaat atatccacca gattgtggtt
     3061 gtagggcagg accgcaagac actcggtgcg ctcattgtac cggcgaaaga agcagtatta
     3121 aaatatgccg aagaacgtaa gatcagcctg ccggcagaga tacgcgattg gccgctcaat
     3181 gcagagattc agaagctcta caagaccgag atcaaagacc gcgtctcaga aaaggccggc
     3241 ttcaaaaact ttgaaaaggt tacgaccttc gccgtgattc ccgatgagtt taagattggt
     3301 gaagagctga cacagaccct gaaggtacgg cgcaacgttg tctttgacaa gtacgcgaag
     3361 cagatcgacg aaatgtacaa gtaaggcaat tcctggctgc gggcttatgc ctgcagtcag
     3421 atagtgcgca gcaggccttc aagctgtgct gaaacgcttt ggccgcgccg ctgcatcatg
     3481 cgcccggcgt cgcgaataaa aagtcccctg tcgtaactac ctgctctgcc ccaggtgaag
     3541 ggggcaatat cacccgacca gtggctgtcg ccgaacaggt tagctcccac cccgacaatg
     3601 ctgccggtgc ctagtaatgt gccgatggcg gtcttggcat agtcgccgat gatcgaacca
     3661 aacttgatcg tacccgtcgt gtaagttttt cctgaatagt gcagcctgat ttcgccgtag
     3721 ttattcttca ggtctgatgt cgtcgtcagt gcacccaggt tcacccagtc gccaaccagg
     3781 gaatgcccca aaaagccgtc gtggtgcttg ttcgaaaaat cgccaatgat cgaatcggca
     3841 atttcgccac cgaggcggca gttctcaccg gcgatactat tcgagaaacg gcactggtcg
     3901 agctgcgtgt ttctgccaac ataggcggga cccttgacga agctcagtgc gctgatgcgc
     3961 gcctggctgt cgatgacgac agggccttcg ctgctatcaa tcacagcgtg gcgtgaaact
     4021 ttcgcttcgg ggtgtacata gagctgctca acgctgcctt cgagctgcag atccttaggg
     4081 tggaggtatt tttcgggtag gagcctgcag tcctgggtga tacgttcgcc gatgcgggaa
     4141 agcagatctg caggttgcag cgggttattt gccggggcct cgtcgaaatg accaaggccg
     4201 tgcaacgcgc gtaactgcgc gagctcctgt gcggggcgtt ggctcgagat acgcagcccg
     4261 cggtcatgtt tcagcagcac gcgttcgaac gggttcagta tgccgtcgcg cagaagaaag
     4321 aggggtttaa gagtgacgag cggcctgagc gtctcttcaa agcagtcgtt cagggtacgg
     4381 tcaggcacta ttttatagtc tgtataaggt caaacaggct gtagaaatac gaacccaggc
     4441 tgtggccgct gagcgccgag tgcgtgccaa tcatcagcaa tgatgcgccg acgggaatcg
     4501 tgaggttatc gtctagtaaa ccctcgtggc tgacaaactc gatcagtagc gcggcaattg
     4561 ccccgatgaa cagcatcacc cagttttcga ggcggatgcc gtggttatcc cacagaaggt
     4621 attctgacgt cacacctgct gcagatatga ggatgagaaa aatcaaccct gcaagaaaag
     4681 ctccgccgat acccccgagt gtcccctgca ggctcttgcc gttataaaag cggatggtgc
     4741 cgtacttgcc gcccacgagg gccgcggccg ggtcaccaaa agtcaggaac agcaccgaca
     4801 ggatcgcgat gtcgcgcggg aaaaaaccga cgaccacaca aagcgcaagg atgtacggca
     4861 cgctgccgtg catcttgtcc atttctttgc ccttaagcat cgggcccgcc accttgacaa
     4921 acaggtcttg ccagaactgc cagcggaagc ggagaaactc gatcagcagg aaaaagactg
     4981 tgatgacgcc ggtaatccag aagatgagcg cgcgagtcgt ctctttaaat acccatgggc
     5041 tgatggggtc gagccaggta aaaaaatagc ctacgggtat caccagcccc agcaagtgga
     5101 aaagtttgcg cgcgtagtta aatttttctc caggcttatt gtctttgctg aagagtttct
     5161 tggtttctga agacatagat taatccagta gtttttcggc gcccagccgg ttgggagcat
     5221 acgagaacat ctcggtgaac ccggtcatgc tgatcacctg ctgcacgcga gccgaaggct
     5281 gaatgagtac cagcttcttg ttgccgcgcg agagctgttc gctcatttca aacagcagcc
     5341 cgatgcccgt tgaaacaata agctccacct cgctcaggtc gagcagtaca tggctgcgcg
     5401 ttgccgcatc gagaaagcgg cggcgcactt catgcgtatt ctgcgagttc atcgcaccct
     5461 tcaccttaaa gaaggtgtac gtctgggctt tttcttcaga gacgttaaaa tgttgctcag
     5521 ccggggtcat ccggctggca atctgtcagg catagccaga cctgcaagga aaaaaagcag
     5581 ggtttgcccc gccggtcatt cgcacttgac gcgacgcacc aacgcagaag taggcgggta
     5641 tgcttgccct cgtcaggtcg atcatctatg ccggcgccga tcttgatccg caccgccgcc
     5701 ggtatgtcat caataccaat gtatactcgc tcatcgtcat tctcatctcg atgccgtacc
     5761 ccttcgtgta tctttacctc aaggttgaca tcgaacctat tttgctttcg ggcagtctca
     5821 tttttgtcag tagctacgta atcgtcattt tgctcaatta cttcaagctt catatgctgg
     5881 ccaagctgtt gtttttcggt acggccgtct gccagctgtt cggtatctgt attgtcttcg
     5941 gtaaagaaac cggtctcgac cagttcttct ttgcggcatt cgtggcagtt ttttttgttc
     6001 tcgaccggca ccagaaaatc ctgatctttc ctgcgttggc cgggtttgtt gcagagtacc
     6061 tgttgataca atatatactg gcttacatcg acccggttgc caacgtcacc ggagactctg
     6121 cccgtatcat ccgatatatt acctcgggtg ccgtattcgc catgctcgca gcgcagtgcg
     6181 cgcatttcta ctacagtaac ctgaagggtg agctcgaaat agaaaaggct cgtcaccagt
     6241 ccgataaact tctgctgaat attctgcctg aaccgattgc cgatgaactc aagcgcaccg
     6301 gttctgttga gcccgtcgaa catgactcag tgaccgtggt attcaccgat tttgtggggt
     6361 ttaccgcgag ctcagaattt cgcaatcctt ctgagctgtt gcgcgagctc gacgactgct
     6421 tctcggcgtt tgatgcaatt atcgaaaagt acggtctcga aaagctcaag acaatcggcg
     6481 atgcttacat gttcgccggg ggagttccgg tgaatctcga agagaacgcc gtgcgctgtg
     6541 tccaggccgc gcaggaaata gtagccttcg tcgcgggaag acgtacccat gacccccgct
     6601 tctggcagat tcgcgtgggc atgcacacgg gtaaacttgt cgccggtgtt atcgggcgca
     6661 aaaagttcat ctacgacgtt tgcggtgata cggtcaatac cgcgagccgg ctcgaagccg
     6721 ccggtgaacc ggggcgaatc aacacatcag aggaaacgta caatcttata aaccaaacgt
     6781 atcgctgcga ataccgtggg gagatttccg tgaagggcaa aggtgctttg cccatgtact
     6841 ttgtcggcga cccggtctga atgcaaccgg gccggttagg tcaagccatc aaactttcgg
     6901 tcagttcgag gttactgtac acgttctgca cgtcgtcatt gtcctcgagt gccgatatca
     6961 gctcgagcgc ttctttacct ttttcaccgt cgacggcgat agtcgtttgc ggaatgtact
     7021 tgagtcctga ctcgacaatc ttgtagccct tggcggtgag tttgggctcc attgcagcga
     7081 gcactgcatg aaagtcgttg accgcggtct tgacggcgaa taccttttca tcgtcttttt
     7141 caatgtcttc ggcgcccgca tcgagcgcga tttccatcac atcatcttcg gtaatgttat
     7201 cgccctcgac cagaatgagt cccttcttgt caaagagata ggcaacagcg ttcgcttcgg
     7261 ccatcgtgcc gccattggtc ttgaaggtac tcttcagctc aggcacagtg cgcgacttct
     7321 tgtcggtcag tacttcgacc atgatggcga taccgccggg gccataaccc tcgtagacgc
     7381 cctcttcgta ggttacgccc tcgagttcac ccgtaccttt cttgatggcg cggtcgatat
     7441 tatccttcgg catcgagttc tgccgagcct tcagtaccgc ggtgcgcagg cgcgggttgg
     7501 cagattcgtc accgccgccg agctttgccg caaccatgat ctctttcgcg agcttggtga
     7561 agagcgcacc gcgctttgcg tcagctgcac ccttgcggtg ctggatattt ttccatttac
     7621 tatggcctgc catgcgcgga ttaatcgcgg cggcgattcc agacgaaaag tagaaacgcg
     7681 gcccgggggc ctacgagaga atcttctgta gcagctcggc ttctgtgact tcgagctttt
     7741 ctcctgactg gcgcagcttg agttcaatct tacccgattc gacggtcttt ttgccgacag
     7801 tcacgataaa aggaaacccg acaagctccg cgtcgttgaa tttcacgcca gcgcgttcgt
     7861 cgcggtcgtc gaggtagacg ctgatgccct tggcctgcag cgttgcatac agcttgtcgg
     7921 cgtaggcagt ttctgcttca cccttcgcga gcgataccag gtagacaagg aacggagaaa
     7981 ggttgcggtg ccagaccagg ccgcgttcgt catggttttg ctcaatggaa gtcgccatcg
     8041 tgcgaccgac accgatgccg tagcagccca tcgtgggcgt gagcggtttg ccgttcgggt
     8101 cgagcaccga gagccccatc ttctctgagt atttcttgcc gagtttgaag atatggccaa
     8161 cttcaatacc gcgcgtttca gtcagttcgg ccttcttgca ttgcgggcag atgtcgcctg
     8221 catgtgcgtt gattaggtcg tggccctgtg cgatcgtgaa gtcccggcct tcggcgacgt
     8281 tcttcgtatg aaaataagtt ttgttcgcgc cggcaatcag gccactgcgc cctctgaggt
     8341 ggttgtcata gaaaatacga agcaccttct taccgacctt gtcgccgtct ttatacttca
     8401 gccgaacagg ccctgcgtat ccagggtcag cacccgtgat tgtcttaatt gtttcttcag
     8461 tggcaagcca gtattcggcc ataccggaca ggttcttcat ttttacttct gaaagttcac
     8521 ggtcgccctg gataaagcag agtacaatag tggtgttgtc ttcgtagatg acagctttga
     8581 tgaagttgcg cggagcttca ccggtgagtt tggccacctc atcaatggtc ttgagttctt
     8641 tggtctcgaa gtcttcaatg cccgcctcga ctgcgctcgg cgagcggtcg gtgacgccgc
     8701 ttgcggtgtt ttccaaaaac gcggtttttt cctggtttga ccggtagccg catttttcgt
     8761 cgctgcagag cagcagggtc tcttcgccga tcgaagaagc taccataaat tcttcagaac
     8821 cactgccgcc catgttgccc gagtcggcct cgaccggaat cgttttcaat ccacagcgtt
     8881 cgaagatggc gcggtaagct tcgcgcatct tctggtacgt ctcatcgagc gacttgtcat
     8941 cgacgtgaaa ggagtaggca tctttcatca cgaattcgcg gcagcggatc aggccgtaac
     9001 gagggcgaat ctcgtcgcgg tacttgcggc cgatctggta caggttaatc ggcagctgtt
     9061 tgtacgactg caggaagctg cgcgcgaggt cggtcatcgc ttcttcgtgc gttggcccca
     9121 gagcgtaacc tacctcgtgg cggtcttgca tgcgcatcat ttctttgccc atgacgtccc
     9181 agcggccaga ttcctgccac agctcagcgg gagtgattac gggtaacgtg acttcgaccc
     9241 cgccaaagcg gttcatctct tcgcgtataa tctgctcgat cttgcggtgg accatgtcgc
     9301 cgaatggcag atagacatag agcc
//
LOCUS       DAOQJF010000102         9251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_3960_length_9251_cov_5.739126, whole genome shotgun sequence.
ACCESSION   DAOQJF010000102 DAOQJF010000000
VERSION     DAOQJF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_9251_cov_5.739126"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /gene="rpsI"
                     /locus_tag="PLF85_07055"
     CDS             complement(<1..230)
                     /gene="rpsI"
                     /locus_tag="PLF85_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNE19458.1"
                     /translation="MENRPESRRTETSATLAESMTKRKASLKKDEETTKQVRRQRKAT
                     DQFVGRRKTSVARVSLRPGSGKILVNNRDVNE"
     gene            complement(244..687)
                     /gene="rplM"
                     /locus_tag="PLF85_07060"
     CDS             complement(244..687)
                     /gene="rplM"
                     /locus_tag="PLF85_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNE19459.1"
                     /translation="MDPLKGQRTHFPKRAELTHKWVVVDAKDMILGRVSTKIATLLMG
                     KNKKNFQPGQLVGDHVVVINASQVKLSGNKATQKEYIWHSRYYGGMKTRPFLKQMEVE
                     PEKAVLLTVKGMLPKTKYGKKLLTRMRVFPGAEHTLQGQQPAPVK"
     gene            complement(817..3870)
                     /locus_tag="PLF85_07065"
     CDS             complement(817..3870)
                     /locus_tag="PLF85_07065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19460.1"
                     /translation="MARAADGKIDLFNTPFAETPIVELNGNWEYYPNRLIEPSGFAKH
                     AAERRFAHVPQSFNLYADSSLRLPASAAATYRLELTLPAQIANYTLRIPPIHTASKIY
                     VNGHLESQAGTITENLSDIRAATRMKYLQLPLAGKTELVIHVANKDFNRGGISQPISI
                     ATAVNMERYRLEALVRDVVVSVALITFGLYHILLFFLRHPESASMYYGVFCLIVGIMR
                     LFNGETIIADLYPSLQWAKRVRFEYVSHFAEAMFLTLYLKNFFPDFRRSSLIRSMVII
                     AGTFATVSLLLPFQWMCELREPMQIFMAAILPFAMYKLALLAWRSVYGARPLMIGIIV
                     LGGIFVSEVIHRTDLHSGPMLFSYATLGFTFLQAILISGKSLRLFHENAELSLRLLRS
                     DKLRDEFLEHTSHELRTPVQAMVQTIENVRRGLSGAVSEQVQRTLAVVEESGQRLMYL
                     IDDLRDFIRLKHSDLRLNLQTVSLRKVIDPVLKLGLGLTEDKQFALIDEIPEGLEDVQ
                     VDPVRVQQILLNLLNTAIRYSHSHAITVKLGKIDGQLAISVLYNGADPDPRYTETLET
                     ENEEVGPLVTQKLAELMGGKYLYHKFAESQHALSITIPYEHIDALEGIIARSRNRTEH
                     RKTEESQGSPLLKGADTSVAGADKVLVVGDHSGQNRLLQEQLASLGKAVTIVKNGAEA
                     MLRLQNRHDISLVICDVLLSDVSGIELTMNIRTQFDIGLLPVILIIDSNQAGVAASAF
                     SAGVNDLIRHPFEKAEVIARVRNLLLQREASLARENYRALNRELEIARSIQESIIPTS
                     QPRSGHYKIEAVCMPARSIGGDFYDFVEDENTVAVLIADVAGHGIPAALYAAMLKIAF
                     HNLRDKARFPEKLLKELNDVMVDRGERTFISCAYTLVDFKNKRLLHANAGHLPLLVQE
                     PGKKTVKRIHPPGGVLGIRKTAAITVEMQHLQPQTRLVLFTDGVIEMTNRHGQFFDEE
                     RLVQVLEEMREAPLADVKEKLLTTLREFAEGEAFLDDVTFVLLDV"
     gene            complement(3942..6041)
                     /locus_tag="PLF85_07070"
     CDS             complement(3942..6041)
                     /locus_tag="PLF85_07070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19461.1"
                     /translation="MIAAILNFYRQMTKLATLIVGFYAAPLLHVVFTSVFAAAGFAYG
                     FARGHLAEIHETTLESLLAGLAIIVVIEIYLNDGFHRLGIRTFIPRVRFVNSMFEEGR
                     LRIDKTVAEYEKLLWYLTDYGKMRAFFGPFHVAVVIGTVLVAEYAWLHTGHSDIYIQT
                     ALVTLPIHVLFVYVTGDLHMGRYRSLVKRQLFFMGHEPPNAFSISLKWKFASIMSVIA
                     ISDYILISLLRSDVAKTGSGYHYIIGFSLFSLALLMVLTVLFFSNIFASIKELQVAAI
                     GLQEGHDPDFYSRASDRELATLSSGFFHAAQKVLNYRRDLEHQIREATAHLSQANAEL
                     QQKDKEIQTELDFAAEIQKEMIPQQHAPWNAVQFGILYRPMQKVSGDFLNVYKKDQAI
                     FVLLADVSGHGVPAALITMAANDAFAAAIRQSDSPAAVFREVNSQLSEQIKTQDYLTA
                     FLVRIDEKLRLTYANASHQKALHYHRKTHTIEAYDTAGLFIGAMSDATDSYEEKTSRL
                     EFGDLLVLFTDGITEQANPRGEEFGHNRLQDLILQHNEQSPQAIADLVHDELLRFAEG
                     ARIRDDVSMIVLRVHPHYRQFIEKFNETVQAMKRRMLDDSSRLLEDLWKLYPEFPALP
                     FLSARIYYQLREFELAEKHVKMHLEKYPGDVRATQLLAAIAIKTGNRSRAVNLVNRLA
                     KANAEDRVTQHLKKKLK"
     gene            complement(6038..7708)
                     /locus_tag="PLF85_07075"
     CDS             complement(6038..7708)
                     /locus_tag="PLF85_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE19462.1"
                     /translation="MFLEAAGSVLESGEHSVVLLSGNVRDLYLAGDATGGGAKFQTLS
                     EALRSQVFAGYDTVAEYDPQGGIYFADAKQRESYLRSLSGYDAYHKTNFAQNPPKDPV
                     QAFSILDNLARLHLLDKKSFAVVIHYLDHIIPTGLNNNNELRYLVIALDKWAQNPEFR
                     RNKIRFVLIAPEVARLESFIAKNAFIEHVSIARPSLEDRTKTITASPYGAGSDGEIQK
                     LAELTSGLTNRQILEVLGQKANDDLTLRAIKRELISSECRGLLEIVEPKHSLSAVAGH
                     EKVKEILSLTAEAIREGKTQYLPMGFLICGPVGTGKTFITECFAHDAALPVVKFLNFR
                     SQWQGETEANLEKIFSILKSIYPVAVMIDEADAFLGNRDAQGDSGVSMRVFASLAALM
                     SDTTYRGKIIWFLMTSRPDLLPVDMKRQGRAEEHLALFHPQSSAELESLYQSVCRKLK
                     IAPPVAEIQPLLDKERSYSGADIEAMLTRSALAAEVKHKTALSTDIMKETITNFRSPE
                     YPLAIELQTLVAVRECTHQDMVPEKYRSLPAAEIEARINELLAMLRQK"
     gene            complement(7769..8185)
                     /gene="tnpA"
                     /locus_tag="PLF85_07080"
     CDS             complement(7769..8185)
                     /gene="tnpA"
                     /locus_tag="PLF85_07080"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNE19463.1"
                     /translation="MPHVTYRLYFHVVFSTKRRLPLLTQGLIADIRNTVQDLSIEIGF
                     QAIATGGYEDHLHLLLSMPPHMNLATVIKRIKGRISREHENLYWQTGYYAESVSDSGV
                     ANLTDYIQHQWQKHKLRALEDAGFFEPIFSPPLPPN"
     gene            complement(8580..8885)
                     /locus_tag="PLF85_07085"
     CDS             complement(8580..8885)
                     /locus_tag="PLF85_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE19464.1"
                     /translation="MYILECSDGTFYTGSTRDLQTRIEQHNRGEGARYTARRLPVRLV
                     YSEEYERIDQAFYREKQVQNWSHQKKESLVEGRFDELPRQAGCRNLSHHKFRNLREV"
BASE COUNT         2020 a         2452 c         2537 g         2242 t
ORIGIN      
        1 tattcgttca catcgcggtt attcacaagg attttacctg agccgggtcg cagtgaaaca
       61 cgtgctactg aggtcttgcg gcggcctacg aactggtcgg tagccttacg ctggcgacgc
      121 acctgcttgg tggtctcttc atctttcttc aggcttgcct tgcgcttagt catcgactca
      181 gcgagagttg ccgaagtttc cgtccggcgc gattctggtc tgttttccat attctacctt
      241 atattatttt actggagctg gctgttggcc ctgcagggta tgctcggcac cgggaaatac
      301 acgcatgcgc gtcagcagct tcttgccata cttggtcttc ggcagcatgc ctttaacagt
      361 cagcagaact gctttttccg gctctacttc catctgtttc aggaatgggc gtgtcttcat
      421 accgccgtag tagcggctgt gccagatata ttctttctga gtggctttgt tgcccgaaag
      481 ttttacctga gaagcgttga taactacaac gtgatcgcct accagctggc ctggttggaa
      541 gtttttctta ttcttgccca tcagcagtgt ggcaatcttg gtgctgacgc gaccaagaat
      601 catgtctttc gcgtcgacaa ccacccattt gtgggttaat tctgcacgct taggaaaatg
      661 cgtgcgctga cctttcagag gatccatcgc gccaaaaacg gcatttcaga tatagcgtcg
      721 agcaaattgc aggggaccgg taaacggcca taacctgggc ggagctatcc ctgttccagg
      781 gagcagcgcg ccgtattatg tctctttatc ccgattctag acgtcaagca gaacaaaggt
      841 tacatcatcc aggaatgctt cgccttcggc aaattcgcgc agtgtggtga gcagtttctc
      901 tttgacgtcg gccagcgggg cttcgcgcat ttcttcgagt acctggacca ggcgttcttc
      961 gtcaaagaac tggccatgcc tgttggtcat ttcgatgacg ccgtcggtaa agagcaccag
     1021 ccgtgtctgt ggctgcaggt gctgcatttc gacggtgatg gctgccgtct tgcgtatacc
     1081 cagtacgccc cccggcgggt gaatacgttt cacggtcttc ttgcctggtt cctgcaccag
     1141 caatggcaag tggcccgcat tggcgtgcaa gagccgtttg tttttgaaat ccaccagggt
     1201 gtaggcgcag ctaatgaagg tacgttcccc ccggtcgacc ataacgtcgt tcaactcttt
     1261 cagcagtttt tcgggaaatc gcgccttgtc gcgcaggttg tggaaagcga tcttgagcat
     1321 cgctgcgtag agtgcggcag gaatgccatg gcctgcgacg tcagcgatca gcacggccac
     1381 agtgttttcg tcttcgacaa aatcgtagaa gtcgccaccg atactgcgcg ccggcataca
     1441 gaccgcctcg atcttgtagt gaccgctgcg tggttgcgaa gtcgggatga tggattcctg
     1501 tatcgaacgc gcaatctcga gctcgcggtt cagcgcgcgg tagttttcac gcgccaggct
     1561 cgcctcgcgc tgcagcagca ggttgcgcac tcgggcaata acctctgcct tctcgaacgg
     1621 gtgcctgatc agatcgttca ccccggcaga gaaggcgctg gcggcgaccc ctgcctggtt
     1681 actgtctata atcaggatta ccggcaagag gccgatatcg aactgcgtcc ggatattcat
     1741 cgtcagctca atacccgaga catctgacag taggacgtcg cagatcacga ggctgatatc
     1801 atgacggttc tgcaggcgca gcatcgcctc ggcgccgttc ttgacgatcg tgacggcctt
     1861 gccgagcgaa gccagttgtt cctgcagcag gcggttctgc ccggaatgat cgccgacgac
     1921 gagcaccttg tctgcgccgg ccaccgatgt gtcggctcct ttgagtaatg gcgatccctg
     1981 ggactcttcg gtcttgcggt gctcggtgcg gtttcggctg cgggcaatga tgccttcgag
     2041 cgcatcgatg tgctcgtagg gtatcgtgat gctgagagca tgctggcttt ccgcgaattt
     2101 gtggtagagg tatttgcctc ccatgagttc ggccagcttt tgcgtcacca gcggcccgac
     2161 ttcttcgttt tccgtttcca gtgtttcggt gtagcgcggg tcggggtctg ccccgttgta
     2221 gagcacgctg atcgcgagct ggccatcgat cttgccgagt ttgaccgtga tggcatgcga
     2281 gtgcgagtaa cgtattgcgg tgttgagcag gttaagcaaa atttgctgca cgcgaaccgg
     2341 gtcgacctgt acatcttcaa gcccctcggg aatctcgtcg ataagggcga actgcttgtc
     2401 ttccgtcagg ccaagcccaa gcttcagaac ggggtcgatc accttgcgca gcgaaactgt
     2461 ctgcaggttc aatcgcaggt cgctgtgctt gagcctgatg aaatcccgca agtcgtcgat
     2521 gaggtacatc agtcgctggc cgctttcttc gaccaccgcc agcgtgcgct gtacctgttc
     2581 gctgaccgcg ccgcttaggc ccctgcgcac attctcgata gtctgcacca tcgcctgaac
     2641 gggagtgcgc agttcgtgag aagtgtgctc cagaaattca tcgcgtagtt tgtctgagcg
     2701 cagcaagcgc agcgatagct cggcattttc gtgaaacagg cggagcgatt tgcccgatat
     2761 cagaattgcc tgcaggaaag tgaagcccag tgttgcgtag gaaaatagca ttgggccaga
     2821 atgcagatct gtgcggtgaa tgacttcaga aacgaaaatg ccgccgagta ctataatgcc
     2881 gatcatgagt ggacgggcgc catagacgct tcgccaagcc agaagcgcca gtttgtacat
     2941 ggcaaaaggc aatatagccg ccatgaaaat ttgcatgggt tcacgcagtt cacacatcca
     3001 ctgaaagggc aataagagag agacggtggc gaatgttcct gctatgatta ccatcgatcg
     3061 aatcaggcta ctcctgcgaa aatcaggaaa gaaattcttc aggtagaggg taaggaacat
     3121 cgcttcagca aagtgactga cgtactcaaa tctcacgcgt tttgcccact gcagggaggg
     3181 atacagatct gcgataattg tttcgccgtt aaagagcctc atgatgccca cgattaagca
     3241 gaatacgccg taatacatcg aggcgctttc agggtgtcgc aggaaaaaca gcagaatatg
     3301 gtagagtcca aatgtgatga gagctacgct caccacaacg tcccggacaa gcgcctcaag
     3361 ccggtaacgc tccatgttaa ctgccgtggc gatactgatc ggttgcgaga ttccgccacg
     3421 attgaagtct ttattagcaa cgtgaataac gagttctgtt ttccctgcga gcggaagctg
     3481 tagatacttc attcgggtag ccgcccgaat atctgaaaga ttctcggtta tggtgcccgc
     3541 ttggctctcc aagtggccgt tcacataaat tttcgaagct gtgtgtattg gcggtatccg
     3601 cagcgtgtag ttagcgatct gcgctggcag agtcagttca aggcggtacg tagcggccgc
     3661 acttgcaggc agcctcagag agctgtccgc atacaggttg aagctctgcg gcacatgtgc
     3721 aaacctgcgc tctgctgcgt gttttgcgaa acctgacggt tcaatgagcc tgtttggata
     3781 gtattcccaa ttgccattca gttctacgat aggcgtttcg gcgaagggcg tattgaacag
     3841 gtcgatcttg ccgtcagcgg cacgggccac cgttaccccc cggccgcaac ctgccaatac
     3901 gacgagcagg aaaaatatca gacctttgtg cctcaaaccg gtcatttcag tttctttttc
     3961 aaatgttgcg tgacacggtc ttcagcgttg gcctttgcca gccggttgac gaggttgacg
     4021 gcgcgcgacc ggttgccggt cttaatcgct atggcagcga gtagttgtgt tgcccgcacg
     4081 tcacccgggt atttctccag gtgcattttt acgtgcttct cggccagctc aaattcacgc
     4141 agctggtagt agatgcgcgc cgacagaaag ggcagagcag gaaattcagg gtagagtttc
     4201 caaagatctt cgagcaagcg cgatgagtca tcgagcatgc ggcgcttcat cgcctgcacc
     4261 gtctcgttga acttttcaat aaactgccgg tagtgcgggt gcacgcgcag cacaatcatc
     4321 gagacgtcgt cgcgaatacg cgcgccttcc gcaaaacgca gcagttcatc atgcacgagg
     4381 tcagcgatag cctgcgggga ttgttcgttg tgctgcagta tcaggtcctg caggcgatta
     4441 tggccgaact cttcgccccg cgggttcgcc tgctcagtga taccgtctgt aaagagcacc
     4501 agcaagtcgc cgaattccag gcgcgatgtc ttctcttcgt agctatcggt cgcatcagac
     4561 atcgcgccaa tgaagagacc agctgtgtcg taggcctcaa tcgtatgtgt cttgcggtgg
     4621 tagtgcagcg ctttctggtg cgaggcattc gcataggtca gccggagttt ttcgtcgata
     4681 cgcaccagga acgctgtgag gtagtcctgc gtcttgatct gctcgctgag ctgcgaattc
     4741 acttcgcgga agactgcagc cggcgaatcg ctttgacgaa tagctgccgc gaaggcatcg
     4801 tttgccgcca tggtgatcag tgcggcgggc acaccgtgcc ctgatacgtc ggcgagcaga
     4861 acgaagatag cctggtcctt cttgtagacg ttcagaaagt ccccggacac tttctgcatc
     4921 gggcggtata gaataccgaa ctgcacggca ttccatggtg cgtgctgctg cggaatcatc
     4981 tctttctgaa tttctgcagc aaagtcaagt tcggtctgaa tctctttgtc tttctgctgc
     5041 agctcggcat ttgcctggct caggtgcgct gttgcttcgc gtatctggtg ttcaagatcg
     5101 cgcctatagt tgagtacttt ctgcgccgcg tgaaagaatc ctgaagaaag agtggcgagc
     5161 tcacggtctg aagcgcgcga ataaaaatcc gggtcatgtc cttcctgcag accaatggca
     5221 gcaacctgca gctccttgat agaagcaaaa atattggaga agaacaagac tgtgagaacc
     5281 atgagaagcg ccagggagaa tagggaaaac ccaataatat agtggtagcc actgccggtt
     5341 ttggcgacgt cggagcgcag cagcgatatc aggatataat cgctgatcgc aatcacgctc
     5401 atgatgctgg caaacttcca tttgagggaa atcgaaaacg cgtttggcgg ttcatgcccc
     5461 atgaaaaaga gctggcgttt gactagcgag cggtaacgtc ccatgtgcag gtcgcccgtg
     5521 acatacacga agagtacatg aatcggtagt gtaaccagcg ccgtctgtat atagatgtcg
     5581 gaatgccctg tatgcagcca tgcgtactcg gccacaagga ccgtgccgat cacaactgcc
     5641 acatgaaacg ggccgaagaa ggcgcgcatc ttgccgtagt ctgtcaggta ccagaggagt
     5701 ttttcatact ctgcgactgt tttgtctatt ctgaggcggc cttcctcgaa catgctgttg
     5761 acgaagcgta cgcgcggaat aaaggtccgg attcccagcc ggtggaagcc gtcattaagg
     5821 tagatttcga tcaccacaat gatcgccagg ccggcgagca gggactcaag cgtggtttcg
     5881 tgaatttcgg ccaggtgacc gcgcgcgaaa ccataggcaa aacccgcagc agcaaacacg
     5941 ctcgtaaaaa ccacgtgcag cagcggcgct gcataaaaac cgacgatcag ggtcgccagt
     6001 ttggtcatct gccggtaaaa gttcaggatt gcggcaatca tttctgtctc agcatcgcga
     6061 gtagctcgtt gatgcgcgcc tcgatttcgg cagcgggtaa cgagcggtat ttttcgggta
     6121 ccatgtcctg gtgggtacac tcgcgcacag cgaccagggt ctgcaactca atcgcgagcg
     6181 ggtattcggg gctgcgaaag tttgtgatgg tttctttcat gatgtcggtc gaaagggcgg
     6241 tcttgtgctt cacctcggca gcgagagccg agcgcgtcag catcgcctcg atgtctgccc
     6301 ccgagtacga acgttcttta tcgagcaatg gttgaatttc ggcaacaggg ggagcgatct
     6361 tcaatttgcg gcaaacactc tggtacaacg attccagctc tgccgagctc tgcggatgaa
     6421 acagcgccag gtgctcttcg gcccttccct gacgtttcat gtctacaggt aacaggtcgg
     6481 gacgcgaagt catcaggaac cagatgatct taccgcggta ggtagtatcg ctcatcaatg
     6541 ctgccagcga ggcaaagacg cgcatcgaaa caccgctgtc gccctgcgca tcgcggttgc
     6601 caagaaacgc atccgcttcg tcgatcatca ctgccacagg gtagatcgat ttcagaatgc
     6661 tgaaaatctt ctcgaggttc gcttcggtct caccctgcca ctgcgacctg aaattcagga
     6721 acttcaccac cggcaacgcg gcgtcgtgcg cgaaacattc ggtgatgaag gtctttcccg
     6781 taccaaccgg cccgcaaatc agaaacccca tgggcaggta ctgcgtcttg ccttcgcgta
     6841 ttgcttcggc agtcagcgaa agaatctcct ttaccttttc gtggcctgcg acggcgctga
     6901 gtgaatgttt tggttcgacg atctcgagca agccacggca ctcagaactg atgagctccc
     6961 gcttgatggc gcgcagggtc agatcgtcat ttgctttctg tccgagcacc tcgagtattt
     7021 gccggtttgt caggccagaa gtgagttcag caagtttttg aatttctcca tcactaccag
     7081 ccccataggg gctggcagtg atggttttcg ttctatcttc cagactcggt cgtgcaatcg
     7141 atacatgctc aataaatgca ttcttcgcga taaaactttc caggcgagcc acttcgggcg
     7201 ctatcagcac aaagcgaatc ttattgcgcc ggaactccgg gttctgcgcc cacttatcga
     7261 gcgcgatcac gaggtaacgc agctcgttgt tgttattcag ccccgtcggt ataatgtggt
     7321 cgaggtagtg gatgaccacc gcgaatgatt tcttgtcgag caggtgcagg cgcgcgaggt
     7381 tatcgagaat cgaaaaagcc tgcaccgggt ctttcggcgg gttctgcgca aagttcgtct
     7441 tgtggtaggc gtcgtagccc gaaagggagc gcaggtagct ctcgcgctgc tttgcatcgg
     7501 cgaaataaat tccgccctgc ggatcatatt ccgctacggt gtcataaccc gcaaagacct
     7561 ggctgcgcag cgcctcgctc agcgtctgga attttgctcc gccgcctgta gcatcacccg
     7621 cgaggtaaag gtcgcgcaca ttgccgctga gcagcacgac agagtgctcg cccgattcga
     7681 gcacgctacc tgctgcttcg agaaacatgg cgcatgggtc aggaattgtc cgtgtggtgg
     7741 aaagcgattc tcccccattg ccaccgaatt aattcggtgg caatggggga gagaagatgg
     7801 gttcaaaaaa gcccgcgtct tcaagtgccc gcaacttgtg tttctgccat tggtgctgaa
     7861 tatagtctgt caaattagcc accccggaat cggacaccga ttccgcataa tatccggtct
     7921 gccagtatag gttctcatgt tcacgcgaaa ttcgcccttt aatgcgtttg atgaccgtag
     7981 caaggttcat gtgtggcggc atagaaagca agaggtgcaa atggtcttca taaccaccgg
     8041 tggcgatagc ttggaaaccg atctctatgg aaagatcctg aactgtgttc cgaatgtctg
     8101 caatcaggcc ttgcgttaat agcggcagtc tgcgtttagt ggaaaaaacc acatgaaaat
     8161 ataaacggta cgtaacgtgc ggcattatca gtctttcatt tgcccccgaa ttaattcggg
     8221 ggcaaatgaa agaaaaagga ccgctcaccc atgacggcgg cttttttcaa cctctttggc
     8281 gaccatgcga gagaagccta cacccatcaa ataaagaccc gatgtcaatg cgactatgga
     8341 ggctattaac attaacgggc tcatggtcga aatctacccc tcgttgtcta aggcgtcaag
     8401 ttatgtgatt gtgttggcct tctccaccct catcccccaa cctccttcgt tgaagacaga
     8461 gaccatgcgg gtcaccacta agtggagaag acgccttgcc ggtcacctcg actgcgctcg
     8521 gcgaccttta gttcattcag ggcatcatga accggtcgtc gagcgcagtc gagaagaccc
     8581 tacacttcgc gcaaattacg gaacttatgg tggctaagat tgcgacagcc tgcctgccga
     8641 ggcaattcat caaaccgacc ttcaaccaga ctttccttct tctggtgtga ccagttctgc
     8701 acctgctttt cccggtagaa tgcctgatct atacgctcgt attcttcgga atacactaat
     8761 cttactggca gtcggcgtgc tgtatatctg gcgccttcgc cccgattgtg ctgttcgatg
     8821 cgagtttgaa ggtctcttgt actcccggta taaaaggttc catctgagca ttcgagaatg
     8881 tacatgaagc ccatgggacc tcatggtcac ctcgactgcg ctcggcgacc tttatttatt
     8941 ctcgccggca gagaatgaaa cggtcaccgc taatctcttc ggcaatgttt gatccttctt
     9001 gccgtacctg tatcggtcgt cgagcgcagt cgagacgacc ggcaacgtgg acataaaaaa
     9061 acccccgtta ccgggggttt ctctctgcca gcccaaggct gaaagagtat tggttttcgt
     9121 tctaaagatt agaagcggaa accaacgttg atgttcacac cagcgtcggt aataccagtt
     9181 gaagtatacg gacgattcag cgatgttgct gtatcatttg ctgtgtttac tgatgctgaa
     9241 taattggtcg t
//
LOCUS       DAOQJF010000103         9194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4000_length_9194_cov_4.704563, whole genome shotgun sequence.
ACCESSION   DAOQJF010000103 DAOQJF010000000
VERSION     DAOQJF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4000_length_9194_cov_4.704563"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1117
                     /locus_tag="PLF85_07090"
     CDS             236..1117
                     /locus_tag="PLF85_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNE19465.1"
                     /translation="MIRGVGLIGSLVADKLINRGHSVIIVSRTPRRFSERPGQTVIDR
                     GDVEAQRSALETCQTVINLEGKNIAAGVWTKKLKSEILRSRLDSVSHLGSILRRVDNP
                     ALRILQASATGYYGNRGEEVLTEDADPGTGFLADTCVEWETAALKSYKKDHFAILRIA
                     PVLSNKAGVFLVWRRIFRSFLGGKLGKGTQYVSWIHEHDMAEMLVHYALNFRPGIINA
                     TAPGPVTNAQLTDVLAASLHRPALFTVPQFVLSALPGDFGKEMLLHSVRAVPKRALAD
                     GFEFRFASFAEAVSDLA"
     gene            complement(1149..2318)
                     /locus_tag="PLF85_07095"
     CDS             complement(1149..2318)
                     /locus_tag="PLF85_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE19466.1"
                     /translation="MPRVSVSDRYIALGHWLQRRLLPGRRLRVAVCDELGLWHIYPEG
                     HLTHKPGGFHTIPGMKRVAVHPPEPARKKAAAERELISAKLETVDLYYRLRRQRVMHE
                     AEKRLLTATDRRTRRRIRDDAEKRIGLFSQYSHDLKTPLSMLTVPLEQLVIEDDSVPV
                     PLRLQLEKIRTAIYSVLRTVTHSLDAARLVTRRQKPMLIPQDFTEFVRQVADVYSLVF
                     ESYGIRLKMNLEPLVICEIDPIQFEKILNNLLSNALKHNMPGGEVLITLKTENHRAVL
                     LVSDSGLGPGTEPGRRRNRNPWAFSSHGFGLSIVRELVRLNRGTMQFSSRTGVGTTVT
                     VVLPTVPELAAAAERGRRHNFNFTMHEVELMAAERNALSRRRRTGDESGGLKVGR"
     gene            complement(2377..5742)
                     /gene="mfd"
                     /locus_tag="PLF85_07100"
     CDS             complement(2377..5742)
                     /gene="mfd"
                     /locus_tag="PLF85_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNE19467.1"
                     /translation="MAESLPARLVKALEPVVAEVRSGNGDLSLPVSLLPLLAFLAHNP
                     AQKKSARQLWVVSDDATALDFVRAWNSLHALSHTDTEYAALVSWGVIPYSFTAPDKEK
                     EYQRTRAEFLMRSELPCLVVASIEGLTQFIAADTDPHAHARLVTPGSNVNRQELTEYL
                     AACGYTHQNPVTKPGEFCVKGSIIDVFPPDEDSPVRLDFFGDEVETIKAFSHETQRSF
                     GSLDEVRISALRPPITGLEKEIADFLAKQKSLKGELPPVLTTHGSNLAGYADLYPALR
                     QCLPAYALWKEQVFICDEEAISARLNTLNTERRYLYERSDSPVKIAPAELFADAAGTA
                     EFLSKGTRFLPEAEDDAAQWSATPRYGGRLGLLKDYLDSREAGSDVFYFIGSEGQRER
                     LSSALTDYPQPQILPWYYPHGFRAPGITVFTENDIFGRVVKKYAADKNISRLIESYTD
                     LKEGDYVVHVNYGIGRFAALRRMRVQNTERDFLELRFADEDKLFVPLDQLNLVHKYIG
                     STENPRLDHLGKKSSWAKTRARVKRIVDSIAQELIELYAQRLEQKGFNFPPDSSFQHD
                     FEAAFPFTETEHQLEAIQAIKQDMESDKAMDRLVCGDVGFGKTEVAIRAAFKAAMAGK
                     QVVVLCPTTILAFQHYRSFSKRFADYPVRIDYISRFRTAGEIAETKKKLAEGKLDIVI
                     GTHALLSDDIKYKSLGLLIVDEEQRFGVGHKEKLRQLRHNLDCLALTATPIPRTLHMS
                     LSGIRDLSIIETPPADRRKIETHVLQENEEVLRLAIKHELDRGGQVYVLHNKVKTIEE
                     QALRLRSLAPRARVAVLHGQMPENEIEEIMVDFYQHAYDLLVSTTIIESGIDIPNVNT
                     LVVLGAHEFGLSQLYQLKGRVGRSDRQAYAYFFYPPPSISPLSNEAERRLEVLAEYDD
                     LGSGFRIAMKDLEIRGAGNLLGKEQSGEILEIGFELYSQMLNDKIQELRGQKRESDDF
                     NCAVTLPCDWYFPDDYIADTRAKMEFYKSISSAQNTEEYLNIREALTDRFGKPPEIVE
                     LMLLFEEIRHLANALRLEKIALDTETGTPYFIVAPTHRLDMERVSSLLTRDKRVRLDT
                     TNAKRINLQLQMTPQVALFRELRGLLRFLGSLA"
     gene            complement(5735..6265)
                     /locus_tag="PLF85_07105"
     CDS             complement(5735..6265)
                     /locus_tag="PLF85_07105"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNE19468.1"
                     /translation="MSSNTFEGPTIVLHRPQIPQNTGNVSRLCVALNAPLVITGRPAF
                     RLDEASVRRAGLDHWQHLNFTHRSSFARFWSENQGRRFVMVTKAGAASALEYNYRADD
                     IIVFGNETMGLPPAMLRKFPESIYLPMSGPVRSLNLSNSVAVVAYEFVRTVHGSTARH
                     DAGTYPRTYYRETRRG"
     gene            complement(6246..7184)
                     /locus_tag="PLF85_07110"
     CDS             complement(6246..7184)
                     /locus_tag="PLF85_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNE19469.1"
                     /translation="MDEAAVLAFIARLKSSRRNLLPADDIGLLPGSGRFYAATDSMVE
                     NTHYRDPWLGPGDIAYKLFARNWSDFLCKGIRPESALLNLSLRRRPDAEEFTKKFLRS
                     LDALLSRYGITLVGGDATRAEHDHFTLSFFGRQGKFIPRAAQNLRAGDLVLQLGAVGA
                     SDAARAVLMEQPEYREKVTLGFRRPHLFQQLPAFAQLLASIDQSDSVAKSLLLLAQAN
                     RAELHVELSQLQVAPAYSSLCSNKPGLLPAAAEDLAVFALARATRGTTRGPHSNRSAA
                     FRPVGQVKTIRAKKPCVRYFWHEEEILPRVDEFEHF"
     gene            complement(7193..8005)
                     /gene="proC"
                     /locus_tag="PLF85_07115"
     CDS             complement(7193..8005)
                     /gene="proC"
                     /locus_tag="PLF85_07115"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNE19470.1"
                     /translation="MKIGIVGLGKMGSALAQALAGKYEITGFEKSEQSVKNLRPSLTK
                     KIRLAGSMEQLVEESTTIIIATKPAQVTQVVEAIPDHRLIISIAAGVALKSIDAARSA
                     NGPTIRAMPNTPMMIRRGITAFTGNKFCTEEDITVVRELFENTGEAIYLEQEKLLHAV
                     TGLSGSGPAYVYLFLQNLEDAGVLLGLSRADSRKLAISTVLGSAELVRQSHEAPQQLI
                     HDVTSPGGTTAVALKSLHRHNFPNAVQEAVTAATRRSVELALEAKSANKDGD"
     gene            complement(8002..>9194)
                     /gene="asd"
                     /locus_tag="PLF85_07120"
     CDS             complement(8002..>9194)
                     /gene="asd"
                     /locus_tag="PLF85_07120"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770661.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNE19471.1"
                     /translation="SAFALGAIASAVWIVILERMLFTLGRRNLIWPARILFVLLFALS
                     TRFSDLGAKVAVSALLFSLLAIIFLTALVTKVREVRKEALDYFGMYPHKHDLAVKFYV
                     LILRLMPKNMVSKLNGFLAGLRLPRFMMTPIILAFARIYKINIQEAELEIRDYESLNN
                     FFTRALKNGSRLIDQKENVIISPVDGRLLVSGSLEQGQLLQSKGMESSLQNLLGNNRF
                     YKQFLGGRYMTIYLSPQDYHRIHSPVEGRILGYYYQPGKLFPVNNLAVNTINQLFSKN
                     ERLITYIQSEFGLVAVVKVGATSVGKIRVTYDPNIATNRWVRLVKEHMYDEEINIRKG
                     AELGRFEMGSTVMLLFERDTAELLEFEEKQKLLFGQPIGLFNGKKAPAARVAKSPAKK
                     GARK"
BASE COUNT         1913 a         2499 c         2683 g         2099 t
ORIGIN      
        1 aagcacctgg tattctttga tttcggcgaa atggatgatt gcgtaagcga tcaaggccaa
       61 agcgccgtag acgaatgcgc gtccgacagt caggataacc tggtcgagaa tcgctgcaac
      121 aagtcggcgc cagaagcctg cgtacacggg gtcgctcata agcgattatc ggatagaatg
      181 gaaaaagtct tgatcggtgg gaaaagctcc tgtagctgcc ggtatggcag cagtgatgat
      241 tcgaggcgta gggttgatcg gttcgctcgt tgccgataaa ctcattaacc gcgggcattc
      301 ggtcatcatt gtcagcagga caccacggcg tttttcagag cggcccgggc agacggttat
      361 tgaccgcggg gatgttgaag cccagcgcag tgctctggaa acctgccaga cagtcatcaa
      421 cctggaaggc aagaatatcg ccgccggggt ctggaccaaa aagctgaaaa gcgaaatact
      481 gcgcagcagg ctcgattcgg tctcgcacct cggcagtata ctgcggcggg tggacaatcc
      541 tgcgctcaga attctgcagg cttctgccac cgggtattat ggcaaccgcg gtgaagaagt
      601 cctgaccgaa gatgccgacc caggtaccgg ctttctggcc gatacctgcg tcgagtggga
      661 gaccgcggca ctcaagtcct acaagaaaga ccactttgct atcctgcgta tcgcgccggt
      721 cttgtcgaac aaggcgggcg tgttcctggt ctggcggcga atcttccggt cttttctcgg
      781 tggtaagctc ggcaaaggta cgcagtatgt ttcgtggatt catgagcacg atatggccga
      841 gatgctcgtt cactacgcgc tcaatttcag gcccggtatc atcaatgcga cggcgccggg
      901 gccggtgacc aatgcacagc ttaccgacgt gctcgcggcc agcctgcatc gaccggctct
      961 ctttacggtg ccgcagtttg ttctttctgc gttgccgggt gatttcggca aagaaatgct
     1021 tttgcacagt gtcagggctg tgcctaagcg tgcactggct gatgggtttg aattccgttt
     1081 tgcatcgttt gccgaagccg tctcagacct tgcctgaatg atctgtcgtg cgcgcagatg
     1141 acttcgtgtc agcgcccgac cttgagtcct cctgactcat cgcccgtacg ccttctgcgt
     1201 gaaagcgcgt tgcgttcggc tgccatgagc tccacttcgt gcatggtgaa attaaagtta
     1261 tgacgccggc cgcgctctgc cgccgcagcc agttcaggta ctgttggcaa caccaccgtt
     1321 acggtcgtgc ccacgccggt gcggctgctg aactgcatcg tgccccggtt cagccgtacc
     1381 agttcacgca ctatggagag accaaaaccg tgcgaagaaa acgcccatgg attccggttt
     1441 cgcctgcgtc ccggctctgt acctggtccc agtccactgt ccgagacgag gagcactgcc
     1501 cggtgattct cggtcttcag ggttatcagc acttctccgc ccggcatgtt gtgctttagg
     1561 gcgttactca gcaggttgtt gagtatcttc tcgaactgga ttggatcaat ctcacagatg
     1621 accaatggct caaggttcat ctttagccga ataccataac tttcgaatac cagcgaatac
     1681 acatcggcta cctgccggac aaactccgta aaatcctgcg gaatcagcat cggtttttgc
     1741 ctgcgtgtca cgaggcgagc cgcatcaagc gaatgcgtga ccgtacgcag cacagaatag
     1801 attgccgtac ggatcttttc cagctgcagg cgcagcggta cgggaaccga gtcatcttcg
     1861 atcaccagct gttcgagcgg aaccgtaagc atcgaaagcg gggtcttgag gtcgtgtgag
     1921 tactggctga agagcccgat gcgtttctcg gcatcgtcgc ggatgcgccg ccgtgtccgg
     1981 cggtctgtag ccgttagcag ccgtttttct gcttcgtgca tgacccgctg ccggcgcagc
     2041 cggtagtaga gatcgacggt ttccagtttt gccgagatca gctcgcgttc tgcggcggct
     2101 ttttttcttg ctggttcggg cggatgtacc gccacgcgtt tcatacccgg aatcgtgtga
     2161 aatcccccgg gtttgtgcgt gagatggcct tcagggtaaa tatgccaaag ccccagctca
     2221 tcgcatacgg cgactctgag gcgccggccg ggtaacaacc tgcgttgcag ccagtgtccg
     2281 agcgcgatgt acctgtctga gaccgagacg cgcggcatcg gagctatttt gcattagcgg
     2341 caaactgcgg caacagaaaa ttatggcagc ttcgatttaa gcgagcgaac ccaggaagcg
     2401 cagcagcccc cgcagctcac gaaacagtgc cacctgcggc gtcatctgca actgcaggtt
     2461 aatgcgcttg gcattcgtcg tatccagccg cacccgctta tcgcgcgtga gcagcgagga
     2521 cacgcgttcc atatcaagcc tgtgtgttgg cgcaacgata aagtacgggg tgccggtttc
     2581 cgtgtcgagg gcaatttttt cgaggcggag cgcatttgcg agatgtctta tctcttcgaa
     2641 cagcagcatg agctcgacga tttccggcgg cttgccaaaa cggtcggtga gcgcttcgcg
     2701 gatattcagg tattcctcgg tattctgtgc cgacgaaatt gatttgtaga attccatctt
     2761 ggcgcgggtg tcggcaatgt aatcgtccgg gaaataccag tcacagggca gcgtcaccgc
     2821 gcagttgaaa tcatctgatt cgcgtttctg cccgcgcagc tcctggatct tgtcattgag
     2881 catctgcgaa tagagttcga aaccaatttc gagaatttca ccgctctgtt ctttacccag
     2941 caggttgccg gcaccacgga tctcgaggtc tttcatcgcg atgcggaaac ctgaacccag
     3001 gtcgtcgtac tcggcgagca cctcaagcct gcgttccgct tcattgctca gcggcgaaat
     3061 cgatggcggg ggataaaaga aataggcgta agcctgccgg tctgagcggc cgacgcgacc
     3121 cttgagctgg taaagctggc tcaggccaaa ctcatgcgcg ccaagcacca ccagtgtatt
     3181 cacattgggg atgtcgatgc ccgattcgat gatcgtcgta ctgaccagca ggtcgtaggc
     3241 atgctggtag aagtcgacca tgatctcttc aatctcgttt tccggcatct ggccatgcag
     3301 taccgcaacc cgcgcccggg gtgccagcga cctgaggcgc agcgcctgtt cttcgattgt
     3361 ctttacctta ttgtgcagca catatacctg cccgccgcga tcgagctcgt gctttattgc
     3421 cagccgcagt acttcttcgt tttcctgcag tacgtgcgtc tcaatcttgc ggcgatcagc
     3481 aggtggagtt tcaataatgc taagatcgcg aatgcctgaa agcgacatgt gcagcgtacg
     3541 gggaatcggc gtggccgtga gcgcaaggca gtcgaggttg tgccgcagtt ggcgcagttt
     3601 ttccttgtgg ccgacaccga aacgctgctc ttcgtcgacg atgagcaagc caagcgattt
     3661 gtatttaatg tcgtcgctga gcagcgcgtg ggtgccaatc acgatgtcga gtttgccttc
     3721 tgccagtttc ttttttgtct ccgcgatttc acccgcggtg cggaagcggc tgatatagtc
     3781 gatacgcacc gggtaatcgg cgaaacgttt actgaagctg cggtagtgct ggaaggcgag
     3841 aatcgtggtc gggcaaagga caactacctg cttaccggcc atcgccgctt tgaaggcggc
     3901 gcgaatggcc acctcggttt taccaaaacc cacatcgccg cagacaagac ggtccatcgc
     3961 cttgtcagat tccatgtcct gtttgatcgc ctgtatggct tcaagctggt gctcggtctc
     4021 ggtgaaaggg aacgccgctt caaaatcgtg ctggaagctg gaatcggggg ggaagttgaa
     4081 acccttctgc tccagccgct gcgcgtaaag ttcgataagt tcctgtgcga tcgaatcgac
     4141 gatgcgtttg acgcgtgcac gagttttggc ccaggatgac tttttgccga ggtggtcgag
     4201 ccgcggattt tccgttgaac cgatgtactt gtggaccagg ttgagctggt cgagcgggac
     4261 aaaaagtttg tcttcgtcgg caaaacgcag ttcgagaaaa tcgcgctccg tattttgcac
     4321 ccgcatgcgg cgcagggcgg caaaacgacc aataccgtag ttgacgtgta ctacgtagtc
     4381 accttctttc aggtcggtat aactttcgat cagtcggctg atatttttgt cagctgcata
     4441 ctttttgacc acccgcccga agatgtcatt ttcagtaaaa accgtgattc ccggggcgcg
     4501 aaaaccgtgc gggtaatacc acggcagaat ctgcggctgc gggtaatctg tcagcgcgct
     4561 gctgagcctt tcgcgttgtc cttctgaacc gatgaaatag aagacatcgc tgccggcttc
     4621 ccttgaatcc aggtaatctt tgagtaaacc caacctgcca ccgtagcggg gtgttgccga
     4681 ccactgcgcg gcgtcgtctt ctgcctcggg cagaaacctg gtgcctttcg agaggaattc
     4741 cgctgtgccg gcagcgtcag caaaaagttc tgcaggcgca atctttaccg ggctgtcaga
     4801 acgctcataa agatatctcc gctctgtgtt gagggtgttc agcctcgccg atattgcttc
     4861 ctcgtcgcaa atgaagacct gttctttcca caaggcgtag gcaggcaggc attgcctgag
     4921 tgcggggtag aggtcggcgt agcctgcaag gttgctgccg tgagttgtca ggacgggagg
     4981 cagttctccc ttcaggctct tttgtttggc gaggaaatcc gcgatttctt tttccagccc
     5041 ggtaataggt ggccgcagcg cagaaatacg tacctcgtcg agcgagccaa aactgcgttg
     5101 tgtttcgtgc gagaacgcct tgatcgtctc aacctcatcg ccgaaaaaat ccagccgcac
     5161 aggcgaatct tcatcgggcg ggaaaacatc gatgatacta cccttgacac aaaattcgcc
     5221 gggtttcgtc acggggttct ggtgggtata accgcatgcg gccaggtatt ccgtcagctc
     5281 ctgccggttg acgttgctgc ccggcgtgac cagccgcgca tgcgcgtgcg ggtcggtatc
     5341 ggctgcaata aactgcgtga gaccttcgat cgaggcgacg accaggcagg ggagttcaga
     5401 gcgcatcagg aactcggccc gcgtgcgctg gtattctttc tctttatcag gagccgtgaa
     5461 actataagga atcactcccc aggaaaccag tgcagcgtac tcggtgtcgg tatgcgaaag
     5521 tgcgtgcagt gaattccacg cccggacaaa atcgagcgca gtcgcatcat cggagactac
     5581 ccaaagctgc cgggcagact tcttctgtgc agggttgtgt gctaaaaagg cgagtagcgg
     5641 cagcagtgag acgggcaggg ataaatcccc gttgccgctg cgcacctcgg caaccacggg
     5701 ctcgagcgcc tttaccagtc gtgcgggtaa tgactcagcc acgccgcgtc tcccggtagt
     5761 aggtacgcgg atatgttccg gcgtcatgtc gggctgtgct gccgtgcacg gtgcgcacaa
     5821 attcgtacgc gacgacggcg acactgttcg acaggttgag gctgcgcacc gggccgctca
     5881 tgggtaggta tatactctcc gggaatttgc ggagcatggc cggaggcaga cccatcgtct
     5941 cgttaccaaa gacgattata tcatccgccc ggtaattgta ctcgagggca gaggctgctc
     6001 cggctttcgt caccatcacg aaacgccggc cctggttttc gctccagaac ctggcgaagc
     6061 tgcttcggtg agtgaagttg aggtgttgcc agtgatcgag ccccgcgcgg cgcaccgacg
     6121 cctcgtcgag ccgaaaggcc ggccgcccgg taatcacgag cggtgcgttg agcgcaacgc
     6181 agagtcgcga aacattaccc gtattctgcg gaatctgcgg acggtgcaga acgattgtag
     6241 gaccttcaaa agtgttcgaa ctcatcgact cggggcagta tttcctcttc gtgccagaaa
     6301 tagcgcacgc agggtttctt tgcccggata gttttcactt gtccgacggg gcggaaagcc
     6361 gctgaacggt tggaatgcgg accacgcgtc gtaccgcgcg tggcgcgggc gagggcaaag
     6421 actgccaggt cctcggcagc ggccggtaga agaccgggct tattgctgca gaggctgcta
     6481 taagccggcg cgacctgcaa ttgggaaagc tcaacgtgca gttctgcccg gttggcctgc
     6541 gcgagcagta agagtgattt ggcgaccgag tcgctctggt cgatggaggc caatagctgc
     6601 gcaaatgcag gcagttgctg aaataggtga ggccggcgaa agccgagcgt cactttttcc
     6661 ctgtattcag gctgttccat gagtaccgcg cgggcagcgt cgctggctcc aacagcaccc
     6721 agctgcaaca ccaggtcgcc ggctcgtagg ttctgtgctg cgcgcggaat aaattttccc
     6781 tgccggccaa aaaagctcag ggtaaagtgg tcgtgctcag cgcgggttgc gtctccaccg
     6841 accagagtaa tgccgtaacg agaaagcagc gcgtcgagcg agcggagaaa tttcttcgtg
     6901 aattcttctg catcggggcg gcggcgcagc gaaaggttga gcagcgctga ttcaggcctg
     6961 attcccttgc agagaaaatc agaccagtta cgcgcgaaga gtttataagc gatatcgccc
     7021 gggcctaacc atgggtcgcg gtagtgggtg ttttccacca tgctgtcggt tgcagcataa
     7081 aacctgccgc tgcctggcaa aagaccaatg tcatctgctg gcagcagatt gcggcgcgaa
     7141 gattttaacc tggcgatgaa cgcgagtact gcggcttcgt ccacagctta ggtcagtcgc
     7201 cgtctttgtt ggccgacttc gcttcgagcg ccagttctac cgaacgccgg gttgctgcgg
     7261 tcacggcttc ctgtactgcg tttgggaagt tgtggcggtg gagtgatttg agtgctacag
     7321 cagttgtacc gcctggcgaa gtcacatcgt ggatcagctg ctgcggcgct tcgtgcgact
     7381 gccgcacgag ctcggccgaa cccagcaccg tcgaaatggc gagtttgcgg ctgtcggcgc
     7441 gcgaaaggcc gagcaataca ccggcatctt ccaggttctg cagaaagaga taaacatacg
     7501 cggggcctga gcccgaaagt cccgttaccg cgtgcagtag tttctcctgc tcgagataga
     7561 ttgcttcacc ggtgttctcg aacagctcgc gcacaacagt aatatcttct tcggtgcaga
     7621 acttgttgcc cgtgaacgcg gtaataccgc gccgtatcat catgggcgta ttgggcatcg
     7681 cgcgaatggt cggcccattg gcgctgcgcg ctgcatcgat cgacttgagc gcaacacccg
     7741 cagcgatgct gatgatcagg cggtggtcgg gtatcgcttc aacgacttgc gtgacctgcg
     7801 ccggttttgt tgcgataata atggttgtac tttcttcaac gagctgctcc atgctgcccg
     7861 caagacggat ttttttggtc agcgagggcc taaggttttt tacagattgc tcgctctttt
     7921 caaagccggt gatttcgtac ttgcctgcca gcgcctgcgc cagcgctgaa cccattttgc
     7981 cgaggccgac aatgccaatc ttcatttacg cgctcctttt tttgccggag atttagcgac
     8041 ccttgccgcc ggtgctttct tgccgttgaa aaggccaatc ggttgtccaa aaagcaactt
     8101 ctgcttttct tcaaactcaa gcagttctgc cgtatctctt tcgaagagca gcatcacggt
     8161 tgaacccatt tcaaaacgcc cgagctccgc gcctttgcgt atgttgattt cttcgtcgta
     8221 catgtgctct ttgacgaggc gtacccagcg gttggtggcg atattcgggt cataggtcac
     8281 gcgaatcttg ccgaccgagg ttgcaccgac cttgacgacg gcaacaagac caaattcact
     8341 ctggatgtag gtaatcaggc gctcattttt tgaaaacagc tggttgatcg tgttgaccgc
     8401 gaggttgttc accgggaaaa gtttacccgg ctggtaataa taccccagaa tgcggccttc
     8461 gaccggcgaa tggatgcggt ggtaatcctg tggggaaagg tagatcgtca tgtaacggcc
     8521 gccgagaaac tgcttgtaga agcggttgtt gccaagcagg ttctgcagcg aggattccat
     8581 acccttgctc tgaagaagtt gaccctgttc cagtgagccc gataccagca ggcggccatc
     8641 aaccggcgag atgatgacat tttctttctg gtcaatcagg cgtgagccat tcttaagcgc
     8701 gcgggtgaaa aaattattca gcgattcata gtcgcggatt tcgagctccg cttcctggat
     8761 attgatcttg tagatgcgcg caaatgccag gataatcggc gtcatcatga aacgcggcag
     8821 gcgcagcccg gccagaaagc cgttcagctt tgataccata tttttaggca tcaggcgcag
     8881 aatcaggaca taaaatttta ctgccaggtc atgcttgtgc gggtacatgc caaagtagtc
     8941 gagcgcctct ttacgaacct cgcgtacctt tgtgaccagg gctgtaagga aaatgattgc
     9001 gagcagggag aacaagagtg ccgatacggc caccttggcg ccgagatcag agaaacgcgt
     9061 agagagtgca aagagcagca cgaagagtat acgcgccggc cagatcaggt tgcgccggcc
     9121 cagggtgaag agcatgcgtt cgagaatcac tatccataca gccgacgcaa tggcaccgag
     9181 cgcgaaggct gaca
//
LOCUS       DAOQJF010000104         9157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4024_length_9157_cov_5.819051, whole genome shotgun sequence.
ACCESSION   DAOQJF010000104 DAOQJF010000000
VERSION     DAOQJF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4024_length_9157_cov_5.819051"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PLF85_07125"
     CDS             complement(<1..1273)
                     /locus_tag="PLF85_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19472.1"
                     /translation="MSVQGLLRQIRQETTLEIVRLIVPGITPTPDAWEYFWFHLPVAL
                     PGLFSFAFCTFFAFSGFYVWLRDRRNFFMLNFMLMSLGMGALGLILALRALVQDLELL
                     LLLHRIVYPIALLLTGGAMGFNYHLMERRYRIIRYTTWLLWGTIALGTYGLITGKAFT
                     GKWHHYWFGSYPVGSVFLTPWGISGVLGLFVVVVPAMFFFRKTGRNLFRDPIGIGVNL
                     TCLTIGLNLPSLTGVPLFPGANFMFVPMALIAYGVFKKDFFSLNRLLFEKRLLYYLIA
                     FLISVFLLGTGILLMLTLPPRVGSVVYTNPLTFIPVLSGLIVFSLAIYLAGSNPQSRV
                     HMMGALSMMLTGFFTILLTVESLRLPLLINLRLDQLIYLIFAFTPIVQYRFIYLLLEL
                     DTDWRSRIIDVLSLIAALLTLTPFFITDFYEY"
     gene            complement(1364..1774)
                     /locus_tag="PLF85_07130"
     CDS             complement(1364..1774)
                     /locus_tag="PLF85_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19473.1"
                     /translation="MTSLLSRNFLLLAFFGSPLLFGNKSGSQNAIVKEIQSVGQNQFM
                     VLDFVEIRDCNCDSGIKIVNENKKLRRFHFTNNSKIWLLKNAGEYAEATLQQFMDARA
                     GNHFGWGFDAETPFEFRFDSAGKKILEMRQVYLP"
     gene            1865..2335
                     /locus_tag="PLF85_07135"
     CDS             1865..2335
                     /locus_tag="PLF85_07135"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNE19474.1"
                     /translation="MRKLHDPNVKLSYALVLVASPFLWATGNRDITSYHLQTFGTVKF
                     NKHGVAHIVTLDGWIAINRKKQVLYKPFIYDNGPDYVSEGLLRFVENGKMGFVNEAGK
                     KVIKAQFDFVYPFEKGTARFCNGCKTIKDGEHSAMDNESGTWGKIDKKGKIRMP"
     gene            2346..2864
                     /locus_tag="PLF85_07140"
     CDS             2346..2864
                     /locus_tag="PLF85_07140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNE19475.1"
                     /translation="MKKILCFSGSTRKGSTCMAILHTLARRQKAHAEFNIFPDLASLP
                     HFDPNAEDAALAGMVEELRAAVAGADAVIFCTPEYVFSLPAILKNAIEWCVGSTVFND
                     KPTAMVVASGAGEKTFESLALILKTIQCKIMPATMLHLKGVRAKLLNSEINDEEILRR
                     LDELCAQLYLLS"
     gene            complement(2867..3838)
                     /locus_tag="PLF85_07145"
     CDS             complement(2867..3838)
                     /locus_tag="PLF85_07145"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE19476.1"
                     /translation="MPGQKRFLVTGGTGYLGRALAERLQRDGNRVRILDVRTGDLYCE
                     YVAGDILSEADVDKAMRQMDGVFHVAAVVGFWKEKRKWQRMVNVEGTRNVMNAALHHG
                     VPRIVYTSTINTLGYAATQDDVGDEETPYNWGPLDVSYMETKREAEELVIRMVHEQNL
                     PATIVNPATIFGGVGAVGMNANRYIELIRSGQMPAYPTGGTNCVALEDVVEGHVMAFR
                     KGRNGERYILGAENLTYKQLFDFIASELGVAAPAIPLVEGLTSVLANVAEKGFGLLGK
                     EPPFTAEMVRASSRFSFYRNDKARREFGMVFKEFLPYLQRLIRQKYR"
     gene            complement(3928..5304)
                     /locus_tag="PLF85_07150"
     CDS             complement(3928..5304)
                     /locus_tag="PLF85_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19477.1"
                     /translation="MTKRLTPLLLTTLSLAVANCALENGSDEIVGSRKAENVRDLTPV
                     QAKYIVTPTTSTIQFSKQDPLVKEVKAGDVVVSDVNATSPAGYIKKVTAVTETSDTKT
                     IETAPAEITDAVEEGQSRAEGTLNPAGDNSLLKGATYNPLNGQIETLTPQPGVTVYGI
                     TQPVNAVPECKSKRWYVEFNNVNIQNAGTINGCVGLDLKFVMEMKIKWFELRSAEFSI
                     NPSITTKLNTNLNGATFQISRAQVTLTTFHLQPITFVIGYVPVVVIPRIKIVLGVDGK
                     VTASLSTSIEFAASAKAGIAYANKKWTQIKEKSSTYNIVPPVISGSIDAMVYAGPEAT
                     FLFYGVAGPGANLYIYSHFKADWSSVTNQLQRWEIWLGFEAGAHFTVEAFGRTLFKIN
                     EPSILGYEGLVASSDFGGIAPPGTGRITGVVGQALSGHYQRYDGLYSAMSGDFVTVGN
                     LVGTLASK"
     gene            5482..7110
                     /locus_tag="PLF85_07155"
     CDS             5482..7110
                     /locus_tag="PLF85_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE19478.1"
                     /translation="MPQQYDVIFIGSGIGSLAAASLLSQWEDKRCLIIEKHWQAGGYT
                     HAFRRKKKFVWDVGLHYVGQIAEGEQIRRVFDVITQNKLQWNRMPDAFDKFVYPGPDG
                     AVRQAHQPRTSTVFDHRTGVENYRADLEAMFPAEKDNIAGYFQDVDDVLVWFQRNEMG
                     KNLPMLLKWLVPLIKKENPYALMTTRDYLAQRFDDEKLRAIVASQWGDYGLPPSQSAF
                     CIHAILVNHFFNGAAYPVGSAEAIAENIIPIIEAAGGKVITSHAVEEIIVENGKATGV
                     RVKNLRPAEGMPDIVSELQAPIIISCAGAYNTYVKLLKTQETADFAQRIIDFNKRNPP
                     VSHISVYLALKDSPAKLGLKGENLWIYDSWNHEETFRRGTAWVAAGRPPMAYLSTPSL
                     KDPKAEGHTAEIISFAPYEEYIKWKEQPWHKRDEAYKALKEKAAQLLIAYVNERVPGF
                     ADLVDFYEVSTPVTTEYMTSHDQGSIYGLACVPERFDIDKSPWCQVHTPIKNLYLTGA
                     DSTSLGIAGAMMGGVATCAHIVKPWHLPKLLKLV"
     gene            7194..7733
                     /locus_tag="PLF85_07160"
     CDS             7194..7733
                     /locus_tag="PLF85_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE19479.1"
                     /translation="MKKTWMIIAGLALFAAACSSTEKKTEQPVVEQPKAEAQPAVQAR
                     SVDMGAQLDSANEKLAGVAMLSLPAFKASASNDSFQSGAAKALDTAKSVQGDIPEGHV
                     LQITGHGNKHPDESKRSGVQLSEQRAKLVYNYFVKNGFPKEKLAYRGVGSAQNDEKLS
                     REQNRRVTFRIVKAGAAAE"
     gene            complement(7791..8120)
                     /locus_tag="PLF85_07165"
     CDS             complement(7791..8120)
                     /locus_tag="PLF85_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNE19480.1"
                     /translation="MKNTPAGSKAPRRKQTNRSIHDFTFLTNHSHVLICLAKGEEITL
                     REVAMRVGITERAVLAIVKDLTLAKVLSVSKNGRRNVYRVNPQVHLRHPVEQHKTVGD
                     LLKSILD"
     gene            8242..>9157
                     /locus_tag="PLF85_07170"
     CDS             8242..>9157
                     /locus_tag="PLF85_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNE19481.1"
                     /translation="MKQAVAEQGAMGQKSRELEFLLSEGDGIGPEITAAVMEILGAAG
                     IQLRAHKIEIGEKSFLAGHSSGISPRAWKQLRAGKILLKAPITTPRGGGYKSLNVTLR
                     KAFGLFANIRPVKSYPGILPGRPMDMVIIRENEEDTYAGIEHRQTREVTQCLKLITSP
                     GTDRILHYAYRLAALRARKELTLMAKDNIMKITDGVFTSRFEAMKPAFPTVKTNSIII
                     DIGTAKLAVTPENFDVIVAPNLYGDILSDVAAEASGSVGLAGSANLGTDFAMFEAVHG
                     SAPDIAGKGVANPSGMLQAACMMLAYAGF"
BASE COUNT         2233 a         2352 c         2532 g         2040 t
ORIGIN      
        1 cgtattcgta aaaatccgta ataaagaacg gggtcagcgt tagcagcgct gcaatgagcg
       61 aaagtacatc gataatacgg ctgcgccaat cggtatcgag ttcgagcagc aggtagatga
      121 agcggtattg cacgatcggc gtgaaagcga agataaggta aattaactga tccagccgca
      181 ggttaatgag caggggtagc cgcaggcttt cgacagtcag caggatcgtg aagaaccctg
      241 tgagcatcat ggagagtgcg cccatcatgt gcaccctgct ctggggatta ctgcccgcaa
      301 ggtagattgc gaggctgaag acaatcaggc ccgaaagcac cggaatgaaa gtaagcggat
      361 tggtgtagac taccgaaccc acgcgcggcg gtaaggtcag catcagcaga atgccggtgc
      421 cgagtaagaa gaccgaaatc aggaaggcga tcaggtaata gagcagccgc ttctcgaaga
      481 gcagtcggtt gagactgaag aaatcttttt tgaaaacgcc gtaggcaatc agggccatcg
      541 gcacaaacat gaagttcgcg cccgggaaaa gcggtacgcc ggtaaggctt ggcaggttca
      601 ggccgatcgt caggcaggtg aggttgacgc caataccaat cgggtcgcga aacagattac
      661 ggcccgtctt gcggaagaag aacatggccg gtactactac gacaaaaaga ccaagtaccc
      721 cgctgattcc ccatggcgtg agaaatacgg aacctacagg gtagctgcca aaccagtaat
      781 ggtgccactt gcctgtaaag gccttgccag tgatcagccc gtaggttccc agtgcgattg
      841 taccccagag caaccatgtc gtgtagcgga tgatgcggta ccggcgctcc atcaggtggt
      901 agttaaaacc cattgcgcca cctgtcagga gcagcgctat cggatagaca atgcggtgga
      961 gcagcagcag cagttcgaga tcctgcacca gcgcccgcag cgcaagtata aggcccagtg
     1021 ctcccatgcc cagtgacatg agcataaagt tcagcataaa aaagttacgc cggtcgcgca
     1081 gccagacgta gaagcccgaa aaggcaaaaa aagtacagaa agcaaaacta aaaagaccgg
     1141 gcagcgctac cggaagatga aaccagaagt actcccaggc atcgggtgtg ggagtaatcc
     1201 ccgggacaat gagtctgacg atttccagag ttgtttcctg tcgaatttgc cgcagcaaac
     1261 cctgaacgga cattcaccga ctgcgacaag cgaaaaggaa ttccaggcag tgataggcaa
     1321 tatgggctat tgccgactag ttgtctgccg tcggctggaa atattaaggc aggtaaacct
     1381 ggcgcatttc gagaatcttt ttgccggcgg agtcaaaacg aaactcgaaa ggagtctctg
     1441 catcgaagcc ccagccgaaa tggtttccag cgcgcgcatc cataaactgc tgcagtgttg
     1501 cctcagcgta ttcgccggca ttcttcagca gccagatttt actgttgtta gtgaagtgaa
     1561 agcggcggag tttcttgttc tcattcacaa tctttatgcc gctgtcgcaa ttgcagtcgc
     1621 gtatttcgac gaaatccagc accatgaatt gattttgccc gacagactgt atttctttga
     1681 cgatcgcgtt ctgtgatccg ctcttgttgc caaatagcag tggggagcca aagaacgcca
     1741 gcaaaagaaa atttcgagat agaagcgaag tcattgcgca tgcactacaa aaaaaatttc
     1801 gtggggcggc aattctttcg cattacctgc agctgatgcc gtgggggaat tcttgacgtg
     1861 gcagatgcgt aagctccatg atcccaacgt gaagctgtca tacgcgctcg ttcttgtcgc
     1921 tagcccattc ctttgggcga ctggcaacag ggacattact tcttaccatc tgcagacttt
     1981 tggcacagtg aaattcaaca agcatggtgt agcgcacatc gttaccctgg acggctggat
     2041 tgccatcaac cgtaaaaagc aggttctgta caaacccttc atttacgata atggtcccga
     2101 ttacgtgagt gaagggttgc tccgtttcgt cgagaacggc aaaatgggct ttgtcaatga
     2161 ggctggaaaa aaggtgatta aagcacaatt tgattttgtt tacccgtttg aaaaagggac
     2221 agcgcgattc tgcaatggct gcaaaacgat caaagacggc gagcatagcg ccatggacaa
     2281 cgaatcgggc acctggggaa aaatagacaa gaaaggtaaa attcgaatgc cctgaagcaa
     2341 agcctgtgaa gaaaattctt tgcttttcgg gcagcacacg caaaggttcg acctgcatgg
     2401 caattctaca tacgcttgcc cgtcgccaga aagcccatgc ggagtttaac atctttccag
     2461 acctggcctc attgccacat tttgacccaa acgccgaaga tgcggcattg gccgggatgg
     2521 tagaagaact acgcgcagcg gtcgccgggg cagacgcagt gatcttctgc acgcccgaat
     2581 atgtgtttag cctgccggca attctcaaga atgccatcga atggtgcgtg ggctcgaccg
     2641 tcttcaacga taagccgacc gcaatggtcg ttgcttctgg tgccggggag aaaacgtttg
     2701 aatcactggc gctgattctg aaaacgattc agtgcaaaat tatgccggcg acgatgctgc
     2761 accttaaagg cgtcagggca aagctgctta acagcgaaat caatgatgaa gagatacttc
     2821 gcaggctgga tgagctctgc gcgcaattgt atttactttc gtaggattat cgatactttt
     2881 gtctgatcag ccgctgcagg tatggcagaa attctttgaa gaccatgcca aattcgcgtc
     2941 gcgccttgtc gttacggtag aaagaaaacc ggcttgaggc gcggaccatt tctgccgtga
     3001 agggcggctc tttgccgagc aggccaaagc ccttctcggc cacgttggcc agaaccgagg
     3061 ttaatccctc aacgagtgga attgccggtg cggctacgcc gagttcagaa gcgataaaat
     3121 cgaaaagctg tttatacgtc agattttctg ccccgagaat gtagcgctct ccgttgcgac
     3181 cctttcggaa ggccataacg tgcccctcga cgacgtcttc gagcgcgacg cagttcgtgc
     3241 cgcccgtagg ataggcaggc atctgacccg agcggattag ttcaatgtag cggttcgcat
     3301 tcatgccaac ggccccaacg ccgccgaaaa tggtggcagg gttgacgatg gtagccggca
     3361 ggttctgttc gtgcaccata cgaatgacca gctcttcggc ttcgcgcttc gtttccatat
     3421 agctgacatc gagcggcccc cagttatacg gagtctcttc gtcgcccaca tcgtcctgcg
     3481 tcgcggcgta acccagtgta ttgatcgtgc tcgtgtagac gatcctgggc acaccatgat
     3541 gcagtgcggc gttcatgacg ttgcgggtgc cttcgacatt taccatgcgc tgccatttgc
     3601 gcttctcttt ccagaatccg acgacggcag cgacatgaaa gaccccatcc atctgccgca
     3661 tggccttgtc gacgtccgct tctgaaagaa tatcaccggc cacatattca cagtatagat
     3721 cacccgtgcg cacatcaaga atgcgtacgc ggttaccgtc acgctgcagg cgttcggcaa
     3781 gtgcacgacc caggtagcct gtgccgccag taacgagaaa acgcttctgg ccgggcatgc
     3841 gcctgcgtgc cattcatctg ccgcaacgta aagcggcttt tgggggtgat ggtcattggt
     3901 tgccgggctg gcaaccaatg taactactta cttgctggcg agtgttccga caaggttacc
     3961 aacagtgacg aagtctcctg acatggcgga gtacaggcca tcatagcgct ggtaatgccc
     4021 tgacaatgcc tggccgacca cgcctgttat gcgacccgtt ccaggcggcg caataccgcc
     4081 gaagtctgaa gaggcaacca ggccttcata gccgagaatg gacggctcgt taatcttgaa
     4141 caacgttcga ccaaatgctt cgacagtgaa gtgggccccc gcttcaaacc cgagccagat
     4201 ttcccagcgc tgcagctggt tagtgacgga gctccagtca gccttgaagt gtgaatagat
     4261 atagaggttg gcacccgggc cagccacacc gtaaaagagg aaggttgctt cggggcctgc
     4321 atagaccatc gcgtcgatgc tgcccgaaat gaccggtggt acaatgttgt atgtgctgct
     4381 tttctcttta atctgcgtcc atttcttgtt cgcgtatgca ataccagctt tggctgatgc
     4441 cgcaaattca atagatgttg agagcgaggc ggttaccttt ccgtcgaccc cgaggacaat
     4501 cttaatgcgc gggatcacga ctaccgggac ataaccaatc acaaaagtaa ttggctgcag
     4561 gtgaaaggtg gtgagtgtca cctgtgcgcg tgaaatctgg aatgttgcgc cgttcaggtt
     4621 agtattgagt ttggtggtga tcgaggggtt gatcgaaaac tccgcggagc gcagctcaaa
     4681 ccacttgatc ttcatctcca tgacgaactt gagatcgaga ccgacacagc cgttgatggt
     4741 accggcattc tggatgttga cgttgttgaa ctcgacgtac cagcgcttcg atttgcactc
     4801 gggtactgcg ttaaccggct gcgttatgcc gtacacggtt accccgggtt gcggagtgag
     4861 agtttcgatc tgcccgttga gtgggttgta ggttgcgccc ttgagtagtg aattgtcgcc
     4921 cgcagggttc agtgtcccct cagcccgtga ttgaccttct tcgactgcat cggtgatttc
     4981 agcgggagct gtctcgatgg tcttggtgtc gcttgtctca gtgactgccg ttaccttctt
     5041 tatatacccg gctggagaag tcgcgttgac atcggatacc accacgtcgc ccgctttcac
     5101 ctcttttacc agtgggtctt gtttgctgaa ctggatggtg ctggtggttg gcgtcacgat
     5161 gtatttggcc tgcaccgggg tcaggtcgcg cacgttttca gccttgcggc tgccgacgat
     5221 ttcgtctgaa ccgttttcca gtgcacaatt ggccactgcg agtgaaagtg tggtgagcaa
     5281 cagtggggta agtctttttg tcatctgcag tcttcctatt tccctaatct gaatactatt
     5341 ggttccactc cgctatcgag gccaaaaagt tcctggaagg caagaagaat ctataatggt
     5401 gaacgtgtgt ttattattag gcttttcggg gaatgcgcca atattctctc gccgctgcat
     5461 cctaacaggg accgatgccg catgccacag cagtatgatg tgattttcat cggttcgggt
     5521 atcggttctc tcgccgcagc aagccttctg tcgcagtggg aggataagcg gtgtctgatt
     5581 attgaaaagc actggcaggc aggcggttac acgcatgctt ttagaagaaa gaagaagttc
     5641 gtctgggatg tcgggctgca ctacgtcgga cagatcgccg aaggcgagca gatacgccgc
     5701 gtgttcgatg tcatcacgca gaacaagctg cagtggaacc gcatgcccga tgcgttcgac
     5761 aagtttgtct accccgggcc ggatggtgca gttcgacagg ctcaccaacc gcgcacaagt
     5821 acagtcttcg accaccgtac cggagtcgaa aactaccgcg ccgacctcga ggcgatgttc
     5881 ccggctgaaa aagataacat cgcgggctac ttccaggacg tggacgacgt gctggtctgg
     5941 ttccagcgca atgaaatggg caagaacctg ccgatgctgc ttaagtggct ggtgcccctc
     6001 ataaagaagg aaaaccctta cgcactcatg acgacacgcg attacctcgc gcagcgtttt
     6061 gacgacgaaa agctgcgcgc gattgtcgcg agtcaatggg gtgattatgg cctgccgccg
     6121 tcgcagagtg cattctgcat acatgcgatt ctggtgaatc acttcttcaa cggtgcggct
     6181 tatcccgtcg gttcggcaga agccattgcc gaaaatatca ttcccattat tgaggcagcc
     6241 ggcggcaaag tgatcacgag tcacgcggtc gaagagatca ttgtcgaaaa cggtaaagcc
     6301 accggcgtgc gggtgaaaaa cctcaggcct gctgaaggta tgcccgatat tgtctctgaa
     6361 ctgcaggcgc cgatcatcat ctcatgcgcg ggagcttata acacctacgt caaattactc
     6421 aaaacacaag agactgcaga ttttgcgcag cggatcatcg acttcaacaa acgaaatccc
     6481 ccggtgtcgc acatcagcgt gtacctcgca ctcaaagact cacccgcgaa gctgggtctc
     6541 aaaggtgaaa acctctggat ctatgactcg tggaaccacg aagaaacctt tcgtcgcggc
     6601 actgcctggg tagcagcggg acgtccgccc atggcatacc tctcgacgcc ttcattgaaa
     6661 gatccgaaag ctgaggggca cactgccgag atcatcagtt ttgccccgta tgaagagtat
     6721 ataaagtgga aagagcaacc ctggcataaa cgcgacgagg cttacaaagc cctgaaagaa
     6781 aaagctgcgc agttgctcat tgcgtacgtg aacgagcgcg tgccgggttt tgccgacctg
     6841 gtcgatttct acgaagtgtc gacgccggtt accaccgagt acatgaccag ccacgaccag
     6901 ggctcaatct acggtcttgc ctgcgtgccc gagcgcttcg atatcgataa atcgccgtgg
     6961 tgccaggtgc acacaccgat caagaacctg tatcttaccg gtgccgattc gacctcgctc
     7021 ggcatcgccg gtgcgatgat gggcggcgtg gcgacctgcg cgcacatcgt gaagccatgg
     7081 catttgccga agctgttgaa gctggtttaa aggcgtaaag aatttggcct tttcggtata
     7141 tgggaagtat tttaacggga aataaaattc acgatataaa agtgaggagt aatatgaaaa
     7201 aaacatggat gattattgca ggtttggcgc tttttgccgc tgcctgcagt tcaacagaga
     7261 agaaaacgga gcagccagta gttgagcaac ccaaggcaga agcgcagccg gcagtgcagg
     7321 cacgttcagt tgacatgggt gcacagcttg attctgcgaa tgaaaaactc gcgggcgtag
     7381 cgatgctgtc actgccagcc ttcaaggctt cggcaagcaa cgactcgttc cagtcgggtg
     7441 cagcgaaggc gctcgatact gcgaagtctg tgcagggcga tattcccgaa ggtcacgtgc
     7501 tgcagattac cggtcacgga aacaagcacc ccgatgagtc aaagcgcagc ggtgtacaac
     7561 tgtcagaaca gcgtgcaaag ctggtgtaca attacttcgt gaaaaacggc ttcccgaaag
     7621 agaagcttgc ataccgcggg gtaggttctg cgcagaacga tgaaaaactt tcacgggagc
     7681 agaatcgccg cgtgacgttc cgcattgtca aagctggggc agcggcagaa taatttcctg
     7741 aaagggtccg ctcgatgcgg acccgggcag gcctctgttc caggggcctg ctaatccaag
     7801 atactcttca gaagatcacc caccgtcttg tgctgttcta ccggatggcg cagatgtacc
     7861 tgtggattta cccggtacac gttcctacgg ccatttttac tgactgatag cactttcgcc
     7921 agcgtcaggt ctttcacgat ggcaagcaca gcgcgttcgg ttatgccgac acgcatggcg
     7981 acttcccgca gtgtaatttc ttcgcctttc gccaggcaga tcagtacgtg cgaatgattc
     8041 gtcaaaaaag taaaatcatg gatcgatcgg tttgtttgtt tgcggcgtgg cgccttgcta
     8101 cccgcgggag tatttttcat ccacggccac cttaagggaa accactcagc cgctcaatat
     8161 ttttttagcc ggtcagatat tcgtaatata cattacatga agactaattc atgtaattaa
     8221 atgcacaata ggaggcggag tatgaaacag gcggtagcag agcagggggc aatgggccaa
     8281 aagtcccgcg agctggagtt tcttctgtca gagggagacg gtattgggcc ggaaatcact
     8341 gcagcagtga tggagatcct cggggctgcg ggtatccagc ttcgcgctca taagatcgag
     8401 atcggggaaa agagttttct ggcaggccac agcagtggca tttctccccg cgcctggaag
     8461 caactgcgcg caggcaagat tctgctcaag gcgccgataa cgacgccacg gggcgggggt
     8521 tacaagagtc taaatgttac cctgcgcaag gcatttggcc tctttgccaa cattcggccg
     8581 gtcaaatcgt acccgggcat actgccgggc cgaccgatgg atatggtgat tatccgggaa
     8641 aacgaagaag atacttatgc gggcattgaa caccggcaga cacgtgaagt aacccagtgc
     8701 ctcaagctca ttacctcacc gggaaccgac cgcatactgc actatgccta ccgcctcgcg
     8761 gcgctgcgtg cccgcaagga actcacgttg atggcgaaag acaatataat gaaaatcact
     8821 gacggtgttt tcactagtcg gttcgaagca atgaaacccg cgtttccgac ggtcaagacg
     8881 aatagcatca tcattgatat cggtacggca aagctcgccg tcacgccgga aaatttcgat
     8941 gtcattgtag ctccgaacct ttatggtgat attctctccg acgttgctgc cgaagcttcg
     9001 ggttcggtgg gccttgcggg ttcggccaac ctcggcactg acttcgcgat gttcgaggct
     9061 gtgcacggtt cggcacccga cattgcgggc aaaggtgttg caaacccatc gggcatgctg
     9121 caggctgcct gcatgatgct cgcctacgcg ggattca
//
LOCUS       DAOQJF010000105         9153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4029_length_9153_cov_5.036821, whole genome shotgun sequence.
ACCESSION   DAOQJF010000105 DAOQJF010000000
VERSION     DAOQJF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4029_length_9153_cov_5.036821"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLF85_07175"
     CDS             complement(<1..362)
                     /locus_tag="PLF85_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE19482.1"
                     /translation="MKKLMVLLAAVTLTVYCEKKSETTNTPASMSSGQAELKALLDGK
                     ATVVDVRTPGEFASGHHPRAVNIPVDQVQSRIAEFGDKSKPVVVYCASGNRSGRAKQI
                     LQSAGYKDVTNAGGFSDLP"
     gene            complement(375..581)
                     /locus_tag="PLF85_07180"
     CDS             complement(375..581)
                     /locus_tag="PLF85_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE19483.1"
                     /translation="MKKNMGAADRALRLIAAIVIGVLYGLNLISGTVAIVLGVVAVAF
                     FATSLIGWCPLYLPFGLSTCKTEK"
     gene            688..960
                     /locus_tag="PLF85_07185"
     CDS             688..960
                     /locus_tag="PLF85_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNE19484.1"
                     /translation="MGYEDSEARQKLNLRLSRVIGQLQGLQKRIGAGGSDCLADIQQI
                     KAANNALWKVAEAYLEIHLNECVRQNKPATEIQKNLQSVIRATFTM"
     gene            972..1139
                     /locus_tag="PLF85_07190"
     CDS             972..1139
                     /locus_tag="PLF85_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19485.1"
                     /translation="MANPLNLALYVAGGGVVGYLYYRLIGCSTGACAITSRPVPSIIY
                     GAVMGAMLGWR"
     gene            1141..>2795
                     /locus_tag="PLF85_07195"
     CDS             1141..>2795
                     /locus_tag="PLF85_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE19486.1"
                     /translation="MSEATKKKLVIVGGVAGGATAAGRARRLDENAEITLIEGSGYIS
                     FANCGLPYHIGGDIPKRETLLLRTPQDLWNRYRVKALVNTKVTNVDRAKKTVTYVQEG
                     QTHEIPYDKLILSQGGDPMKPAVEGVENVPHFSLRWVEDMDAIIEQMAKAEYKHVAVV
                     GGGFIGIEVAEALRHKHFEVTLVEFAPGVMPNLDPEFSNLAKKELEAHGIKVLTQTTI
                     KKAEKGGALTLSDGSTFNADFIVFAAGVKPETSLARQIGLEIGKTNGVVVNDFMQSSD
                     ENIYVVGDMAEITNRITHTATRIPLAGPANRQGRLAAENALMGNRCRYRGALGSSVIK
                     IFGKTLASTGLTEKAAKAANISAQAVMVHSNNHAGYYPNSERIALKLVFSPADGRVLG
                     AQAFGPNGTEKRIDVIATAIVGWLTIYDLAGLDLTYAPPYSSANDPINMAAFQAMNHH
                     SGLSPVISSAELAARRSEFALLDVRGAGEIKHYPVESDYQIPVDHLRENLSQIPKDKP
                     LALLCQSGQRSYVAQRILKQSGFKEVYNITGGWLGLAAQRGKW"
     assembly_gap    2796..2895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2896..3561
                     /locus_tag="PLF85_07200"
     CDS             <2896..3561
                     /locus_tag="PLF85_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE19487.1"
                     /translation="DIFYMFFNMFLFPLAGYATLSLFFATQLQTWGFVQALRGLIPLQ
                     GLPAWLQIILLFVARDFIQWNVHVILHRVPFFWKLHEVHHSSQIMSFPVHLRYHWAEN
                     VIYAIPEYVLFLMLGRGVNDLFIIYALSLFIGHLNHANLRLPWGFLKYVFNTSELHLW
                     HHAKQIPTRYGVNYAISLSLWDWIFRTAYDPQRPPAAIGLADEPRFPRSFLRQLVWPL
                     GKK"
     gene            complement(3586..5163)
                     /locus_tag="PLF85_07205"
     CDS             complement(3586..5163)
                     /locus_tag="PLF85_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNE19488.1"
                     /translation="MADWYKEAIVYQIYPRSFRDSNGDGIGDLTGIREKIPYLKDLGV
                     DTVWLSPINTSPMLDFGYDISDYRSIDPIFGTMADFDALLAELHENKIRLMMDLVVNH
                     SSDQHPWFIESRSSRDNPKRAWYVWHDGKNGSPPNNWQSVFGGSAWEWDKATKSFYLH
                     SFLKEQPDLNWRNPEVKAAVFAEIEFWLKKGIDGFRLDVVNLYFKDAAFRDNPLRLWG
                     WRYPRPYEFQTHVYDMSQPEMHPLLKDLRVLLDKYNAASVGEVLVDFSGDPELAASYL
                     GDNDELHLTFDFTLLYQKWDAAAMATTLSRWFRACGDNWPTLVFNNHDQDRSYTRYAK
                     NHESDARAKVLAALLLTAKGTPFLYYGDEIGMKNGRIARNELRDPVGIRFWPLTVSRD
                     VARTPMLWHGGQHAGFSTAKPWLPVNDDFRERNAAVQDADPHSILRWHRALLKLRSER
                     AELRLGDWTEIAAGKDVLAYRRSYSGMASEIYMNFSAKPVPLPPVSGTVLLSNCGRTN
                     AAGELMGYEVLVVEARN"
     gene            complement(5165..7444)
                     /locus_tag="PLF85_07210"
     CDS             complement(5165..7444)
                     /locus_tag="PLF85_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE19489.1"
                     /translation="MPPKRTTLTLKQIITGTDLFLNLSRETKEHLADAFKKVTVGKNK
                     VVIQQGEPGDALYLVAQGSFSVFVTTDGKKKKVGEIKPGSCFGEGALVTSEPRNATVV
                     CDEAGIVYRINREDFTKALKDHPEELKAFVRLIGRRSRALHRSVFRPEPRRIKELVSS
                     VSLFASVGDKLIAELEPQMEWIFLPGGETLMRQGDAADGMYVVVNGSLVYEVRNHENR
                     IVSTGNFSKGDIIGEMALLTGEPRTATVIATLSCEIVKISTAAFEAVFSKHPPSMLAI
                     TKLIADRFTKDRMGKRPVKQARSIITLFPLQKELSVVSFAADLKRALKKFGSTAIVES
                     RDFKKRLGKLDYAISDLLELLYDVQDHNEFVILCPDFSDKIWTETILHHTNRILLLSD
                     AQKGQDLTPEEIRYLGDSEDTHGPLRELVLLYAAPDTKPTNTAALTMIRKTDAVRHIK
                     TYAERGFDSLARFITGRSVGLALGGGGAKGLAHIGLLKAMQEENIPIDMIGGTSAGAL
                     MAGIYAMGYDAPEMERIAKALMSDKKTLNDYTIPGVSLIRGKKFNTAIKNFFGETMIE
                     DLWLPYFAVATSLTKAQKTIIDRGPVWKALRASASIPGILPPFYENGELLVDGAMLDN
                     IPGAVMRQRGADLVISVALASQSDAAEDEVFSGIYEENNSGALPSALRMLFKRIIGKA
                     QKQRDVPNLLSLLMRATFVASDAAVAQARVESDIFAELPVEQFGLFDWKKFYQLVDIG
                     YRYGKTHAKSWKKQLGIRG"
     gene            7591..8241
                     /locus_tag="PLF85_07215"
     CDS             7591..8241
                     /locus_tag="PLF85_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19490.1"
                     /translation="MKLLRFCAVALLLPQLVFAGFEIGGDFSYFNPQLTTRIYPALAS
                     RQSTGFSGALYTGYAHFTVALPHVVTIGFGPTLAYTNQNSVDGVTIEEVSISRFGGDA
                     KIQIETIPFLSPYLRLTLGKDTISWIDRGSVGTGRYIAESTAGGLYYSAMLGVQLPFS
                     AEFALYAQAGYTASPGSRMLSRSYAVDGVRQTVNVPGDADTGYSGWVIGAGGRLSF"
     gene            complement(8246..8929)
                     /locus_tag="PLF85_07220"
     CDS             complement(8246..8929)
                     /locus_tag="PLF85_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE19491.1"
                     /translation="MHKAIGNDRKFDPERIARVCEAEKADIIALQEVDNGVPRSRHLN
                     LAEVLGEMLGYHQRLGCNVKLKVGSYGNATLSRWPFAHGQNLDLTWGIKKRRGCLSDV
                     VETPRGPLVVMNFHLGLSRIERHFQLKKILASDFLHSHRSHPMLMLGDSNERTRYLDL
                     FLSSAGFENTAGDAKRKYKTWPSFAPIWNLDKIFHNDKCERIHYEVVKNSLTKVASDH
                     LPVKAVIRI"
BASE COUNT         1949 a         2453 c         2522 g         2129 t
ORIGIN      
        1 ggcagatcgc tgaagccccc ggcgttggtt acatctttat aacctgcaga ctgcagaatc
       61 tgtttcgcac ggccgcttcg gtttccggaa gcgcagtaga caaccacagg ctttgactta
      121 tcgccaaatt ctgcgatacg cgactgtacc tggtctacag gaatattgac cgcgcgcggg
      181 tggtggcctg aagcaaattc acccggagta cgcacatcga ccactgttgc cttaccgtca
      241 agcagcgcct tgagttccgc ctgccccgag ctcatggagg ctggcgtatt tgttgtttcg
      301 gatttctttt cgcaatacac tgtcagtgta actgcggcga gtagtaccat tagctttttc
      361 atagtgttgc ctctttactt ttccgttttg caggtcgaaa gcccgaatgg caggtacagc
      421 gggcaccagc caatgaggct tgtggcgaaa aaagcgacgg cgacaacacc gagcacaatc
      481 gctacggttc ctgaaatcag attcaatcca taaagcacac caatcacaat cgccgcgatg
      541 aggcgaagtg cgcggtctgc ggctcccata ttctttttca taattactcc ttgagaaaaa
      601 tctcaccagg tcgcgggctt gccgccgcct gacagaagca gtataaaaaa cttgccaccc
      661 cccgtcaaat attataccat acccagtatg ggatatgaag acagcgaagc ccgccaaaaa
      721 ctcaacctga ggctgagtcg ggttatcggc cagctgcaag ggctgcagaa gcgcattggt
      781 gcaggcggtt ctgactgcct tgctgacatt cagcagatca aggcggccaa taacgcttta
      841 tggaaagtgg ccgaggctta tttagaaatc cacctcaatg aatgtgtgcg acagaataag
      901 ccggcgaccg agattcagaa gaatctgcag tcggtgattc gcgccacttt cacgatgtaa
      961 taggagaatg aatggcaaat ccactgaatt tagcactcta tgttgccggc ggcggtgtgg
     1021 tggggtatct ctactaccgg ctgatcggct gttcgacagg ggcgtgcgca atcacttcgc
     1081 gcccggtacc ctcaattatt tatggcgctg tcatgggcgc aatgctcggc tggaggtaaa
     1141 atgtctgaag caacaaagaa gaaactggtg attgtcggcg gtgtcgccgg gggagctacc
     1201 gcggcgggcc gcgcgcggcg tctcgacgag aacgccgaaa ttaccctgat cgaaggcagc
     1261 ggttatatct cgtttgccaa ctgcgggctg ccgtaccaca tcggtggcga tattcccaag
     1321 cgcgaaactc ttctcttaag aacaccgcag gacctctgga accgctaccg cgtgaaagcc
     1381 ttggtcaaca cgaaagtgac caacgttgat cgcgcgaaaa agaccgtcac ttacgtgcaa
     1441 gagggccaaa cgcatgaaat cccttacgac aagctgatct tgtcgcaggg tggcgacccg
     1501 atgaagccgg cggtcgaagg cgtggagaac gtaccgcact tctcactgcg ctgggttgaa
     1561 gacatggacg cgatcatcga acagatggca aaggccgaat acaaacacgt ggccgttgtc
     1621 ggcggtggtt tcatcggtat tgaagtcgca gaggcgctgc ggcataagca ctttgaagtc
     1681 acgctggttg aatttgcccc cggtgtgatg ccgaacctcg accccgaatt ttccaacctg
     1741 gcgaaaaaag aactcgaagc gcacggcatt aaggtgctga cacagactac tataaagaag
     1801 gcagagaaag gcggagcgct cactctttcc gatggttcta cctttaatgc agacttcatc
     1861 gtctttgctg cgggcgtgaa gcccgaaacg agccttgcca gacaaattgg cctcgagatc
     1921 ggcaagacaa atggcgtggt cgtgaacgac ttcatgcaga gctcagacga aaacatctac
     1981 gtcgtgggcg atatggctga gattaccaac cgcatcacgc acactgcaac gcgcattcct
     2041 ctcgccggac cggctaaccg gcagggacgg cttgcggcgg aaaatgcact catgggcaac
     2101 cgctgccgtt accgcggagc gctgggttcg tcggtcatca agattttcgg caaaacgctc
     2161 gcctcaaccg ggcttacgga aaaggcagcg aaagcagcaa acatttcagc ccaggcggtg
     2221 atggtacact cgaacaacca tgccggatac tacccgaact cagagcgcat tgcgctgaag
     2281 ctcgtgttct cgcctgcaga cggccgcgtg ctgggtgcgc aggcttttgg tcccaacggg
     2341 acagaaaagc gtatcgacgt gatcgcgacg gcgatcgtcg gctggctcac gatctacgat
     2401 cttgcgggtc tcgacctgac gtatgcgcca ccgtattcat cggcgaacga cccgatcaac
     2461 atggcggcgt tccaggcgat gaaccaccac tcgggtcttt cgccggtgat atcttctgct
     2521 gagctggcgg cacggcgcag tgaattcgcg ctgctcgatg tgcgcggcgc gggtgagatc
     2581 aagcactacc cggttgaaag cgactaccag ataccagtcg atcacctgcg tgagaacttg
     2641 tcgcaaatac cgaaagataa gccactggcg ctgctctgcc agtcgggcca gcgcagctac
     2701 gtcgcgcagc gcatcttgaa acaatcgggc ttcaaagaag tatacaatat cacgggtggc
     2761 tggctggggc tggctgctca gaggggaaaa tgggannnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnngatat cttttacatg ttcttcaaca tgttcttgtt tccactggcg
     2941 gggtatgcca cactgagttt atttttcgcc acacagctgc agacctgggg ttttgtacag
     3001 gctctgcgcg gtctgatacc gctgcagggt ttgcctgcgt ggctacaaat tatcctgctc
     3061 tttgtggcgc gtgacttcat tcagtggaac gtgcatgtca tattgcaccg tgtgccgttc
     3121 ttctggaaac tgcacgaggt gcaccacagc tctcaaatta tgagcttccc ggttcacctg
     3181 cgttaccact gggccgaaaa tgttatctat gctatccctg aatacgtgct ctttctcatg
     3241 ctcggtcgtg gcgtgaatga tcttttcatc atctacgctc tgtcactttt cataggacac
     3301 ctgaaccatg ccaacctgcg tctgccgtgg ggatttctaa agtatgtgtt caatacatcc
     3361 gagctccacc tctggcacca tgctaaacag ataccaacac gctatggtgt aaactatgcg
     3421 atttcgctca gcctctggga ctggatattc agaactgcgt acgatcccca aaggccaccc
     3481 gcggcgattg gccttgcaga cgagccccgg tttccgcggt cgtttctgcg gcaactggtt
     3541 tggccgttgg gtaaaaaatg aaaaggcgcg cggctgtatg taatactaat tacgcgcctc
     3601 cacaaccaac acctcataac ccatgagctc acctgcagca ttggtgcgcc cgcagttaga
     3661 aagcagaacc gtacccgaaa ccggcggtag aggcacaggc tttgccgaga aattcatgta
     3721 gatctcgctc gccatgccgc tatagctgcg ccggtacgcg agcacatctt tgcccgcagc
     3781 tatctcagtc cagtcgccga ggcgcagttc ggcgcgttcg ctgcgcagct taagcagcgc
     3841 cctgtgccac cgcaaaatcg agtgcgggtc tgcatcctgg accgccgcat tgcgttcgcg
     3901 aaaatcgtcg ttcactggca gccacggctt tgctgtcgaa aagcccgcat gctgcccgcc
     3961 atgccatagc ataggcgtgc gggccacgtc acggctcacg gtgagcggcc aaaagcgaat
     4021 gccgaccggg tcgcgcagtt cattacgggc aatgcggccg tttttcatac cgatctcatc
     4081 gccgtaatat aagaagggag ttcctttggc tgtcagtaat agcgctgcga gtactttcgc
     4141 gcgcgcatcg ctttcgtgat tcttcgcata acgggtgtac gagcggtctt ggtcgtggtt
     4201 gttgaagaca agagtgggcc agttgtcgcc gcaggccctg aaccagcggc tgagtgtagt
     4261 tgccatcgcg gctgcgtccc atttttggta gagtagagtg aagtcgaaag tcaggtggag
     4321 ttcgtcgttg tcgcccaggt agctcgccgc aagttcgggg tcacccgaga agtccacgag
     4381 tacctccccc accgaagcgg cattgtactt gtcgagcagc acccgtaggt ctttgagcag
     4441 cgggtgcatc tcgggctgcg acatgtcgta cacatgcgtc tggaactcgt acgggcgcgg
     4501 gtagcgccag ccccagagcc gcagcgggtt atcgcgaaac gcagcatctt taaagtagag
     4561 gttcaccaca tcgaggcgaa agccatcgat accttttttg agccagaatt caatttcggc
     4621 aaaaaccgcc gctttcactt cggggttgcg ccagttaaga tcgggttgtt cttttaagaa
     4681 ggagtggagg taaaagcttt tcgtcgcttt gtcccattcc caggcagagc cgccgaagac
     4741 gctctgccag ttgttaggtg ggctgccgtt cttgccgtcg tgccagacat accaggcgcg
     4801 tttcgggtta tcgcgcgacg aacgcgattc gataaaccac gggtgctggt cagagctgtg
     4861 gttgaccacg aggtccatca tcagcctgat cttgttttcg tgcagttcgg caagcagtgc
     4921 atcgaagtca gccatcgtgc cgaagatcgg gtcgatgctg cggtagtcag agatatcgta
     4981 gccgaagtcg agcatcggcg aggtgttgat cggcgagagc cacaccgtgt cgacgccgag
     5041 atctttcagg tatggtattt tttcgcggat gcctgtgaga tcgccgatgc cgtcgccgtt
     5101 tgaatctcta aagctgcgcg ggtagatttg ataaactatc gcttctttgt accaatctgc
     5161 cacgttaacc ccttataccg agctgctttt tccagctctt tgcatgggtc ttgccatagc
     5221 gatacccgat gtccaccagt tggtagaatt tcttccagtc gaaaagcccg aactgctcta
     5281 cgggtagctc ggcaaaaata tccgactcaa cccgcgcctg cgccacagcg gcgtctgaag
     5341 cgacaaaggt cgcgcgcatc agcaggctca gcaggttggg tacatccctc tgtttctgcg
     5401 ctttgccaat gatgcgcttg aacagcatgc gcagcgcaga ggggagcgct cccgaattgt
     5461 tttcttcata gataccgctg aatacttcgt cttctgccgc gtcactctgt gaagccagcg
     5521 ccaccgaaat gaccaggtcg gcgccgcgct gccgcatcac agcgcccgga atgttatcga
     5581 gcatggcgcc gtcgacgagc agctcgccgt tctcgtagaa cggcggcaga attcccggga
     5641 tcgacgccga ggcgcgcagc gccttccaga cggggccgcg gtcgatgatg gttttttgtg
     5701 ctttcgtcag gctggtcgcg acggcaaagt aaggaagcca aagatcttcg atcattgttt
     5761 cgccaaaaaa attcttgatc gccgtgttaa attttttgcc gcgaatgagt gagacgccag
     5821 gaatcgtgta atcattaagc gtcttcttgt cgctcatgag tgctttggca atgcgttcca
     5881 tttcgggtgc gtcgtaaccc atcgcgtaga tacctgccat cagcgcgccc gcgctggtgc
     5941 caccgatcat atcgatgggt atgttctctt cctgcatcgc cttgagcagg ccaatatgag
     6001 ccagcccctt ggctccgccg ccacccaatg caaggcctac tgacctgccg gtaataaagc
     6061 gtgcgagact atcaaagccg cgttcggcgt aggttttgat atgcctcacg gcatcggtct
     6121 ttctgatcat ggtcaaagct gcggtattcg tcggctttgt atcaggagca gcgtagagca
     6181 acacgagctc gcgcagcggg ccgtgggtat cttcagagtc gccgaggtag cgtatctctt
     6241 caggcgtaag gtcttgaccc ttctgcgcat cgctcagcag caagatgcgg ttcgtatggt
     6301 gcagaattgt ctctgtccag atcttgtcgg aaaagtccgg acagagaatc acgaactcat
     6361 tgtgatcctg cacgtcgtag agcaattcga gcaggtcaga aatcgcgtaa tcgagcttgc
     6421 cgaggcgctt tttgaaatcc ctgctttcga cgatcgcagt gctgccaaat tttttcaggg
     6481 cccgctttag atctgcggca aaactaacca ctgaaagttc tttctgcaga gggaaaaggg
     6541 tgatgatact gcgcgcctgt ttgaccggcc gttttcccat tcggtctttc gtgaaacgat
     6601 cggcaatcag cttggtgatc gcgagcatgc tcgggggatg tttggagaat acggcttcaa
     6661 aagccgcagt gctgattttg acgatttcac acgagagcgt ggcaatcacg gtggccgttc
     6721 tcggttcacc tgtcagcagc gccatttcgc cgataatgtc gcccttagaa aaatttcccg
     6781 tcgagactat gcggttctcg tggtttctca cttcgtagac caggctgccg ttgacgacga
     6841 cgtacatgcc gtcggcagcg tcgccttgcc gcatcagtgt ctcaccgccc ggcaggaaaa
     6901 tccattccat ctgtggttcc agctcggcga tcagtttatc tcccacgctg gcaaaaagag
     6961 agaccgacga aacaagttct ttgatacgac gcggttcggg acggaagaca ctgcggtgca
     7021 gtgcgcgcga tcttctgccg attagccgca cgaaagcctt gagctcttca gggtgatctt
     7081 tcagcgcttt ggtgaaatct tcgcggttga ttcggtagac gataccggcc tcgtcgcaga
     7141 caaccgtggc attgcgcggt tcactggtaa cgagcgcgcc ttcgccgaag cagctgccgg
     7201 gtttgatttc tccgaccttt ttctttttac cgtctgtagt gacgaagacg ctgaaactgc
     7261 cctgtgcgac caggtagagc gcgtcgccgg gttcgccctg ttgtatgact accttgtttt
     7321 tcccgacggt gactttcttg aacgcatccg cgaggtgttc ttttgtttcg cgcgaaaggt
     7381 ttagaaacag atccgttccg gtgataatct gcttcagggt tagtgtggtt cttttcggcg
     7441 gcatggtttc tcaatgcagg gtaaattggc ttcccaaacc gggtctctgc gtcactctga
     7501 gaatcaggtt ttgcagcacc aggttccgga aaaactcccg caccggccga ttgctgttta
     7561 cgccttgttt ctgcggcgcg atattctcgc atgaaattgc tccgattctg cgcagtagcg
     7621 ctgttgctgc cgcagctggt gtttgcaggc tttgaaatcg ggggggattt cagttacttc
     7681 aacccgcagt taacgacgcg catttatcct gcactggcaa gcaggcagag tacaggcttt
     7741 tccggcgccc tttacacagg ctatgctcat ttcactgtcg cgctgccgca tgtggtgaca
     7801 attgggttcg gtccgaccct ggcttatacc aaccagaact ccgttgacgg agtgacgatt
     7861 gaagaagtca gcatatcgcg ttttggcggc gacgcgaaga tacaaataga gaccattccg
     7921 ttcctttcgc cctaccttcg ccttacattg ggcaaagata ccatcagctg gattgaccgc
     7981 ggtagcgtgg gcacaggccg ctatatcgcc gagtcgactg ccggtggact gtattacagc
     8041 gcgatgctcg gtgtgcagtt accattcagt gcagagtttg ccctctatgc acaggcaggt
     8101 tataccgcat caccggggtc acgcatgctg agccgcagtt acgccgtcga cggtgttcgg
     8161 caaacagtta atgtgcctgg cgatgcagac accggttatt cgggatgggt aatcggcgcc
     8221 ggcggcagat tgtctttctg aaaaatcaga tccttatcac cgccttcaca ggcaggtggt
     8281 ctgaagctac tttcgtaagc gaatttttta ctacttcgta gtgtatacgc tcgcacttat
     8341 cgttatggaa aattttgtcg aggttccaga tcggtgcgaa cgaaggccag gtcttgtatt
     8401 tacgtttcgc atcgccggcg gtattttcaa aaccggcgga gctaagaaac aggtccaggt
     8461 agcgcgtccg ttcgttactg tcgccgagca tcagcatcgg gtgtgagcgg tgcgaatgga
     8521 gaaaatccga cgcgagaatt ttctttagct ggaagtgccg ctcgatacgc gaaagcccaa
     8581 ggtgaaaatt cattacgaca agtggtcccc tcggggtttc gacgacgtcg ctcaggcagc
     8641 cgcggcgttt cttgataccc caggtgaggt cgaggttttg cccatgcgca aacggccagc
     8701 gtgacagcgt tgcattgccg tagctgccta ccttgagttt tacattgcaa cccagccgct
     8761 ggtggtagcc aagcatttcg ccgagtacct cggcgaggtt caggtgacgg ctgcggggca
     8821 cgccgttgtc gacctcttgc agggcgatga tgtcggcttt ttcggcttcg cagacgcgcg
     8881 cgattcgttc cgggtcaaac ttgcggtcgt tgccgattgc cttatggatg ttatatgtca
     8941 ggacggtaat cagcaggggc gtaacctatt acacgggcag gggtaaattt ttgactgtcg
     9001 cgtaactgaa aattcgttga cgcgagtcgg caaaacatcc ggttctcacg gcgtggatat
     9061 tacccgttac ctgcaccagc tatccagccg cactgtaaac cggggcttcg tcgtctttaa
     9121 agaaggccag gaaggcgacg gcaatatgta ctt
//
LOCUS       DAOQJF010000106         9099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4060_length_9099_cov_5.174812, whole genome shotgun sequence.
ACCESSION   DAOQJF010000106 DAOQJF010000000
VERSION     DAOQJF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4060_length_9099_cov_5.174812"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLF85_07225"
     CDS             <1..374
                     /locus_tag="PLF85_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNE19492.1"
                     /translation="IGYLISIAIFGLYQAIFMANAGGAWDNAKKLVEVELKAKGTPLH
                     DACVVGDTVGDPFKDTSSVAMNPVIKFTTLFGLLAVELAVEMKGGAETVRSVLSVVFF
                     VLSMVFVWRSFYKMRIQSEKA"
     gene            complement(420..1274)
                     /locus_tag="PLF85_07230"
     CDS             complement(420..1274)
                     /locus_tag="PLF85_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19493.1"
                     /translation="MAKAKRTPPAAEDKFDAYLRDRFAELKRELDAQISAGQPRAGVL
                     QKFTPRGGFSPRGGWTVGIAALLLTAISVPLVMQLNQQREQMRSAVVTSEQPAALPQR
                     DNKPVTEEDEETPAPKHANRQASRRKGEVETERRENEAADSKESGLAFKKRAQPAIMD
                     EGKTDRVALAEEATPRVAPAAPAPAAEAPAGELAKSESNADQFAASGAPAAPAAKTRS
                     APINDAQFRLKQTELAAIEKTEMEQLWKEYEKNPELFSKDKKKTERLKLLLARHDTKS
                     RARRMLTQ"
     gene            complement(1267..1845)
                     /locus_tag="PLF85_07235"
     CDS             complement(1267..1845)
                     /locus_tag="PLF85_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE19494.1"
                     /translation="MRLPGLAFIAFALDQASGFMARLLPYRERLYRLALAQLRNTSDA
                     EDAVQEALIKAGRNAQTFRSESSEYTWVVRILINHCHDVQRKVSRRSKRELSDEALGH
                     KSAQIADSRTRPEQNIELSEVSRHLMEAVNELGETYKQLILMRYFEELSYEEIAAALD
                     INLGTVKSRMNQAKTLLKAKLIERGIGEDVLG"
     gene            1950..2273
                     /locus_tag="PLF85_07240"
     CDS             1950..2273
                     /locus_tag="PLF85_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19495.1"
                     /translation="MIKDPRLMLGLLFLLPLAFSACRKGKLVETAKANAKQYGLARCE
                     CDKLQRQDPPGDITLCFDQMQQAKRYLSFNMEMGKFSTTEKAEVMAHGDKVYQDCMAA
                     KSPSH"
     gene            2319..3800
                     /locus_tag="PLF85_07245"
     CDS             2319..3800
                     /locus_tag="PLF85_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19496.1"
                     /translation="MPPQLTQALEFISAHRYLFIAGGIAFVLLLTAIITLVVAFRRPK
                     TARPKPVVQPESAAKAPVSEVPAPRVNRSRLAVGDIVSPWTQGVYERNSPEAKLGSLL
                     MAFAFDEAEGIRRAQQGDLVAGIDLAELMRNCASASGVAVHGAYATLALWHAGDGRYA
                     EMALKVASHAHMPVEQKDLIRFLFQDIIGIRLKPRNVQGLSHWNNKAFRTALALYTAK
                     RVKESEWKEMSLALPEYLYPYYELELAQKLPPRTIYRRARESRVYGEGLFRLNLETLA
                     RYLNSKRWQRLQNSDKAARFSAWLADQPCRSEAEALLQVANPDVFEAADIDEARAQQI
                     HSVLEGKNPKAWLFDGDKPPRLHYRLQYFKYFCLFQKHNEAVRCFATLGVFRRDRAQR
                     LWYARALFMSGMQHEAWAEMSALMTDFPRDAAVLNEAGIYAHKLGRFDEAAEIFGLAR
                     SYFPDDATIAYNEAVFTEQYSKKQVEEKWSRVQKLTQPPVVEG"
     gene            3893..5377
                     /locus_tag="PLF85_07250"
     CDS             3893..5377
                     /locus_tag="PLF85_07250"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE19497.1"
                     /translation="MKEQPLDVLVMGAGFSGIGVAINLKNQGVENFKIIDLGKDFGGT
                     WRDNKYPGAACDVPSHLYSFSFEQNPNWSRMFATSDEIHKYMLGVAEKHNLRDKAEFG
                     VKVNGLTYDDNTCLWTASTEGGKKYVARVVVSGVGALSVPAIPKIEGSDAFKGEILHT
                     ARWRKDVDLRGKKVVVVGGGASAIQLVPAIAPEVGQLTVFQRTPSWIIPKPDYEIGDT
                     EKSIYRALPFVQQIRRTIIYAMTESFGAGIVFNTPVTKVLERLARWNIERSIKDPELR
                     RKVTPDYRFGCKRMLISNDWYPALTRENVELVASPVTKITPTGVIAEGKEYAADILIY
                     ATGFEVPKAGSPFPIKGARGHDLNTDWKNGAESYLGMTVHGYPNLFYCMGPNTGPGHT
                     SVLVYTEPQYRYIASAVSRMLTEGIAALEVKAEKQADFTSFIDERMKITNWTSGCKSW
                     YLTDGGRNTTLYPGFAGEYVLKASTFHLEDFDLVKKSDIERKVA"
     gene            5520..6701
                     /locus_tag="PLF85_07255"
     CDS             5520..6701
                     /locus_tag="PLF85_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNE19498.1"
                     /translation="MREVYIIGAHETEHGSFKEKNFRHLIAEAGNKAIEDAGIDRKEI
                     QALYLGNYNGNGLNNQNTMAALAATSLAMPHAATLRFEAACASGGVAFRNAMIAVGAG
                     VYDTVMVMGVEKMNIPFDELAPEDQMKAALSGVDYLYEAGPGMNGSTMFAMFAHRHMH
                     EFGTTRKQLAMVAEKNKYHGSLNPYAYKQREVPLEKILKSPIIASPFSAQEVSLITDG
                     AAAVIITTKDRAKALGKKNAVKVMGSGHGGASFSVAFRAKHTESEATKAAAAEAYKMA
                     NLKPSDISVAECHDCFSFTEIMNVEDLGFTEKGKGGFFAEEGHSRLGGKLPINTSGGL
                     TAKGHPIGATGVGQIVEMTWQLQDKSDKRQVKDAKFALTHVLGGPASVSTVHILGRSD
                     I"
     gene            6698..7027
                     /locus_tag="PLF85_07260"
     CDS             6698..7027
                     /locus_tag="PLF85_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNE19499.1"
                     /translation="MSTIPAVKCKDCGTPHPKGIAACPACGSMHLEDYEAKGSGSIYT
                     YTVNTFVPIGKHKARAPYCMAVVETDDKMRITAIVEMPDANTAKIGDKVQFKAYEDKL
                     TPIFTRV"
     gene            complement(7113..8216)
                     /locus_tag="PLF85_07265"
     CDS             complement(7113..8216)
                     /locus_tag="PLF85_07265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE19500.1"
                     /translation="MQRSLTDSFKVVAAIAVVGIHATSQSETRFAQNHNFYSLDFLSV
                     LVNQWARFSVPLFIYLSGYGLAKGDKGIQGGFLASWGNFLWLRLPTILLPYAFFSALA
                     LTLQFQNYHGSMGELWHTVAEKLRTGGADYHLYFLVILAQCYLLFPLLVWAAGRSTAG
                     FRYTSWIALILVSGLLYKGSSEILLGNLNLSHPGWHASFVVYWLPYFMLGILHAAEPP
                     RPLPGLVALLAVLATLVTVVSEYIHYSWQGTPVDYYNHFSRPSVMLYALVVVWWLHSI
                     SAVRGRLKPTPNGVEKLAPLTFSIYLIHPQILRIVNAYLPSLPTFFGWLVVVVTTFLL
                     VHALILVTNKAHEKGPGFLTGPVRFFQRCLGLR"
     gene            8295..9071
                     /locus_tag="PLF85_07270"
     CDS             8295..9071
                     /locus_tag="PLF85_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE19501.1"
                     /translation="MHVFITGAATGIGEATIDAILEQIPSAGFTIADKNQQALQEVCQ
                     KLSRRGVHNHGIACDLTDIDGLPDVVKRAREQMGEVEILVNNAGVMIVEDLARMPWER
                     GKLMLDIHFTAPMKLIYEILPSMLQRGQGGIINIASMAGKALLPGCTWYGGSKAAMGH
                     ASEILYGEVKDRGVHVLTVYPGPISTELERGARQGYEENLVSRLMPVGEREELARRIV
                     AAYLAKQPVLAYPAVYDVARHLHSFSGWLAQVFAPRTKSA"
BASE COUNT         2023 a         2519 c         2624 g         1933 t
ORIGIN      
        1 tcattggtta cctgatttca attgcgattt tcggcctgta ccaggcgatc ttcatggcaa
       61 acgccggtgg tgcctgggat aacgcgaaga aactggttga agttgaactc aaggcaaaag
      121 gtaccccgct gcacgatgcg tgcgtcgtgg gtgacaccgt gggcgacccg ttcaaagaca
      181 cttcgtccgt agccatgaac ccggtgatca agttcaccac gctctttggt ctgctcgcag
      241 ttgagcttgc agtcgaaatg aagggtgggg ctgaaacagt gcgcagcgtg ctgtcagtcg
      301 tgttcttcgt gctgagcatg gtctttgtct ggcgctcatt ctacaagatg cgcatccagt
      361 cagaaaaagc ctgatactgg ccttaaaccc aataaagaag cctgcgtaag caggcttctt
      421 tattgggtca acatgcggcg cgcgcgcgat ttggtgtcgt ggcgcgcgag caagagtttc
      481 agtcgttcag tcttcttctt gtctttagaa aatagttccg ggtttttctc atattctttc
      541 cagagttgtt ccatttcggt tttttcgatc gcggcaagct ctgtctgctt caggcgaaac
      601 tgtgcatcgt tgatcggggc cgaacgtgtt ttggcagcag gtgcggcggg cgcgccgctc
      661 gcagcaaatt ggtcggcgtt gctctcactc tttgccagct cgcctgcagg cgcttcagct
      721 gcgggggcag gtgccgcagg tgcgacgcgg ggtgtcgctt cttcagccag tgcaacgcga
      781 tccgttttgc cttcgtccat aattgctggt tgcgcgcgct ttttgaatgc gagacccgac
      841 tctttgctgt ctgcagcttc gttttcgcgc cgttccgttt ctacctcgcc cttgcggcga
      901 ctggcctgcc ggttagcgtg cttcggggcc ggggtttctt cgtcctcttc ggtaacaggt
      961 ttattatccc gctggggcag tgctgccggc tgctcgctgg ttaccacagc agaacgcatc
     1021 tgctctctct gctggttgag ctgcatgacg agcggtaccg agattgccgt gagtagtaat
     1081 gcagcaatgc cgaccgtcca tcctccgcgt ggagagaagc cacccctggg tgtaaacttc
     1141 tgcagcacgc ctgccctggg ctggccggct gaaatctgcg cgtcgagctc acgctttagt
     1201 tcggcgaagc ggtcgcgcag gtaggcgtca aacttgtcct ccgccgccgg gggggtgcgc
     1261 tttgccttag ccaagtacgt cctcccctat gccgcgttcg atgagtttcg ccttgagcag
     1321 ggtcttggcc tggttcatgc gcgattttac tgtgcccaga ttgatatcga gagccgccgc
     1381 gatttcttcg tagcttagct cttcaaaata gcgcatcagt atcagctgtt tataagtttc
     1441 gccgagctca ttcactgctt ccattagatg ccgcgagacc tcggaaagtt cgatattttg
     1501 ctcgggccgg gtgcgcgaat cggcaatttg tgccgacttg tggcccagcg cttcgtcaga
     1561 aagctcgcgc tttgagcggc gcgagacctt gcgctgcacg tcgtggcagt ggttgatgag
     1621 tattctcacc acccaggtgt attcactcga ttcgctgcgg aatgtctgtg cgttacgacc
     1681 agccttgata agggcctctt gcaccgcgtc ttcggcatcg ctggtattgc gcaactgtgc
     1741 gagcgccagg cggtagaggc gttcgcggta gggcagcagg cgcgccatga agcccgaggc
     1801 ctggtcaagt gcaaacgcta tgaaagcaag gcctggcagg cgcatgaata cagtaggcgg
     1861 gcagcttctc gcctgcagtg tcaatcaagt atatgcggtc atctggtgaa aaccattgac
     1921 gcgccctgcg gcgccggcga tagacgaaca tgattaaaga cccgcgtctt atgctgggtt
     1981 tgcttttctt gctgccgctc gcattttcgg cctgccgcaa aggcaaactt gtggagacgg
     2041 caaaagcaaa cgcaaagcag tatgggctgg cgcgctgtga gtgtgacaag ctgcagcgcc
     2101 aggacccgcc aggtgacata actctctgtt tcgaccagat gcagcaggca aagcgttacc
     2161 tgtcttttaa catggaaatg ggcaagttct ccaccaccga aaaagctgag gtcatggcac
     2221 acggcgacaa ggtgtaccag gattgtatgg ccgcaaaaag cccttcgcac tgacctttca
     2281 gaagccaata ccggcctgcc gataatcgta ttacaggtat gcccccgcag ctcacgcaag
     2341 cactggaatt tatctcggcc caccgttacc tgttcatcgc gggcggcatt gcgtttgtcc
     2401 tgctgctgac cgcaatcatc acgctcgtgg tcgctttccg ccgtccgaaa acggcgcgac
     2461 caaagccggt ggttcagcct gaatctgcag caaaagctcc ggtgagcgag gtgccggccc
     2521 cacgcgtcaa ccggtcgcgg ctggccgtag gcgatattgt gagcccctgg acgcagggcg
     2581 tttacgagag aaactccccc gaagcgaaat tgggcagtct gctgatggca tttgcgtttg
     2641 acgaagccga gggcattcgg cgcgcgcagc agggtgacct tgtggcaggc atagacctgg
     2701 cggagctcat gcgaaactgc gcgagcgcca gcggcgttgc cgtgcacggc gcttacgcaa
     2761 cgctcgctct ctggcacgcg ggtgatggcc ggtatgccga gatggctctc aaggtggcta
     2821 gccacgcgca catgccagtc gaacaaaaag atctcatccg tttcctgttc caggacatta
     2881 tcggcattcg cctgaagccg agaaacgtcc agggcctttc gcactggaac aacaaggctt
     2941 tccgcactgc gctcgcgctc tataccgcga agcgcgtgaa agaatccgaa tggaaagaga
     3001 tgtcgctcgc cctgccggaa tacctttatc cctactacga gctcgaactg gcgcagaagc
     3061 tgcccccgcg cacgatttac cgccgcgcgc gcgaaagccg tgtctatggt gaaggtttgt
     3121 tccgtctcaa tcttgaaacg ctcgcgcggt acctgaattc caaacgctgg cagcgcctgc
     3181 agaattcaga caaggccgcg cgtttctcag catggctggc tgaccagccc tgccgcagcg
     3241 aagccgaagc gctgctacag gtcgcaaacc ccgatgtgtt cgaagctgct gatatcgacg
     3301 aggcacgcgc gcagcagatt cacagcgtgc tcgaaggcaa gaaccccaag gcctggctgt
     3361 ttgacggcga caagcctccg cggctgcact atcgtctgca gtacttcaag tacttctgcc
     3421 ttttccagaa gcacaacgaa gccgtgcgct gcttcgcgac acttggcgtt tttcgccgcg
     3481 accgcgcgca acggctctgg tatgcacgtg cgcttttcat gagcggcatg cagcatgaag
     3541 cgtgggcaga gatgagtgcg ctcatgacgg attttccccg tgacgcggca gtgctcaacg
     3601 aggcgggcat ctacgcgcac aagctcgggc gcttcgacga agcggcagaa attttcggcc
     3661 ttgcgcgcag ctattttccc gacgatgcga ctattgccta caatgaagcg gttttcaccg
     3721 agcagtacag caagaagcag gtagaagaaa aatggtcgcg cgtgcagaag ctgacgcagc
     3781 ctccggttgt tgaaggctga aaaatgaaca cttgttcact ttatgtcttg acgtattgat
     3841 tctcgctctc agatagcgcc cgcaggcgtg tcatgcgttt gatggaggca ggatgaaaga
     3901 acaaccactg gatgtactcg tgatgggtgc cggctttagc ggtatcggtg tcgcgatcaa
     3961 cctgaagaac cagggggtcg aaaacttcaa gatcatcgac ctcggcaaag attttggcgg
     4021 tacatggcgc gataacaagt atcctggcgc tgcctgcgat gtgccgagcc acctctattc
     4081 attctctttt gagcaaaatc ccaactggtc gcgcatgttc gctacctccg atgaaatcca
     4141 caaatacatg cttggcgttg ccgagaaaca caacctgcga gacaaggcag agttcggggt
     4201 aaaggtgaat ggcctgacct acgacgacaa cacctgcctg tggactgcga gcaccgaagg
     4261 cggtaagaag tacgtcgcgc gcgtcgtggt gtcaggtgtc ggagcgcttt ctgtgcccgc
     4321 gattcccaag atcgaaggct cagacgcgtt caaaggtgaa attctgcaca ccgcccgctg
     4381 gcgcaaggac gtcgatctca ggggcaagaa ggttgtggtc gtcggcggcg gtgcgagcgc
     4441 gattcagctg gtgcccgcca tagcacccga agtcgggcaa ctgactgtct tccagcgcac
     4501 accctcgtgg atcataccga aacccgatta cgaaattggc gacaccgaga agtcgatcta
     4561 ccgtgcactg ccgtttgtgc agcagattcg ccgcacgata atctatgcga tgactgaaag
     4621 ttttggtgcg ggcattgtct tcaacacccc ggtcaccaag gtactggagc gcctcgcgcg
     4681 ctggaacatc gaacgcagta tcaaagaccc cgagctgcgc cgcaaggtga cacccgacta
     4741 ccgtttcggc tgcaagcgta tgctcatctc gaatgactgg taccccgcgc tcacgcgtga
     4801 gaatgtcgag ctcgtggctt ctcccgtgac aaagatcact cccactggcg taatcgccga
     4861 aggtaaagaa tacgccgcag atatactgat ttatgctaca ggtttcgaag tgccgaaagc
     4921 gggttcacct ttcccgatca agggtgcgcg cggccacgac ctcaacaccg actggaaaaa
     4981 cggcgccgag tcatacctcg gcatgaccgt acacggttat ccgaatctct tctactgcat
     5041 gggccccaac accggcccag gccatacgtc ggtgctcgtc tataccgaac cgcagtaccg
     5101 ttacatcgcc tcggcggttt cgcgcatgct gacggaaggc atagctgcgc ttgaggtcaa
     5161 agcagaaaaa caggcagatt tcacgagctt cattgacgag cgcatgaaga tcaccaactg
     5221 gacctcgggc tgcaagagct ggtatttgac cgacggcggc cgcaacacca cgctctaccc
     5281 cggcttcgca ggtgaatacg tgctcaaggc gagtacgttt catctcgaag attttgacct
     5341 tgtgaagaag tcagatatcg aacgaaaggt agcttaagaa ccccctcccg gcttcgccgt
     5401 actccccctt agcaggggga gagctttgga gaacctgtcg aaagttatcg acgcgttgtt
     5461 caacttttgc agattgtctg tagcgtttgc tacaatgcgg cggtgccgcg aggagctata
     5521 tgagagaagt gtatatcatc ggtgctcatg aaacggagca cggtagtttc aaagaaaaaa
     5581 actttcgcca cctgattgcc gaagccggta acaaggcgat cgaagatgcg ggcattgacc
     5641 gcaaagagat ccaggcgctt tacctgggca actacaacgg caacggcctc aataaccaga
     5701 acacgatggc agcccttgca gccacttcgc tcgcgatgcc tcatgcggct acgctgcgct
     5761 tcgaagcagc ctgcgcttca ggcggagttg cttttcgtaa cgcgatgatc gccgtcggcg
     5821 caggtgttta cgacaccgtg atggtgatgg gcgtcgaaaa gatgaacatc cccttcgacg
     5881 aactggcgcc agaagaccag atgaaggccg ccctttcagg cgtggattac ctctacgaag
     5941 caggcccagg catgaacggt tcaaccatgt tcgcgatgtt cgctcaccgc cacatgcacg
     6001 agttcggcac gacccgcaaa cagctcgcga tggttgcgga aaagaacaag taccacggtt
     6061 cactgaaccc gtacgcgtac aagcagcgtg aagttccact cgaaaaaatt ctcaagtcgc
     6121 ccatcatcgc ttctcccttc agcgcacagg aagtttcgct gatcactgac ggtgcagctg
     6181 cggtaattat taccaccaag gaccgcgcga aagcgctcgg taaaaagaat gctgtcaagg
     6241 tgatgggttc gggccacggt ggtgcatcgt tcagcgtcgc gttccgtgcg aaacacaccg
     6301 aatcagaagc gaccaaggca gcggcggcag aagcctacaa aatggcgaac ctcaagccgt
     6361 ctgatatttc ggtggctgag tgccacgact gcttcagctt caccgaaatc atgaacgtcg
     6421 aagacctcgg cttcaccgaa aaaggtaagg gcggattttt tgccgaagaa ggccattcac
     6481 gcctcggcgg caagctcccc atcaacacct cgggcggtct caccgcgaag gggcacccga
     6541 tcggtgctac cggcgtcggc cagatcgttg agatgacatg gcagctgcag gacaaatcag
     6601 acaagcgcca ggtgaaagat gccaagttcg cgctgacgca cgtgctcggc ggcccggcat
     6661 ccgtttcgac agtccacatt ctgggaagga gcgacatatg agtactattc cagcagtaaa
     6721 atgcaaggat tgcggcaccc cgcatccaaa aggcatcgcg gcctgcccgg cctgcggttc
     6781 tatgcacctt gaagactacg aagcgaaggg tagcggaagt atctacactt ataccgtgaa
     6841 taccttcgtg ccgatcggca agcacaaggc acgcgcgccc tattgcatgg ctgttgttga
     6901 aactgacgac aagatgcgca ttaccgcgat tgtcgaaatg cccgacgcga atacagcgaa
     6961 gatcggcgac aaagtgcagt tcaaggcata cgaagataag ttgacgccaa tcttcacgag
     7021 agtctgaaag cctcacccca tcccctctcc taaaggagag gggcttaaat ctcccccttc
     7081 tcctttagga gaagggggtc ggggggatga ggttatctta aacctaaaca acgctgaaaa
     7141 aaacgcacag gcccggtgag aaatcccggg cctttttcat gcgctttgtt ggttactaag
     7201 attagtgcat gcaccagcag gaatgtagtc actacgacga ctagccagcc gaaaaatgtt
     7261 ggcaatgaag gcaggtaggc attgacgatt cgcagaatct gcgggtgtat aaggtagatg
     7321 ctgaacgtga gtggggcgag cttttcgacg ccgttagggg taggtttcaa cctaccccta
     7381 acggcggata ttgaatgtaa ccaccagacc actaccaacg catagagcat cacgctcggt
     7441 cgtgagaaat ggttataata atctactggc gtgccctgcc aggagtagtg gatgtactcc
     7501 gacacgaccg taaccagcgt tgcgagtaca gccagcaatg cgaccagccc tggcaatgga
     7561 cgcggcggtt ctgcagcgtg taatatgccg agcatgaagt acggcaacca gtagacgacg
     7621 aacgaggcat gccagcccgg gtggctgagg ttcaggttgc ctaagagaat ctcagagctg
     7681 cctttgtaca gcaaacccga cacaagaatt aatgcaatcc agctggtgta gcggaatcct
     7741 gccgtgctgc gccctgctgc ccacaccagc agtggaaaaa gcaggtagca ttgcgcgagt
     7801 atgacaagaa agtacaggtg gtaatcagcg cccccggtgc gcagcttttc tgctaccgta
     7861 tgccagagtt cacccataga accatggtag ttttgaaact gcagcgtgag tgccagagcc
     7921 gagaagaatg catacggcag caggatggtc ggcagccgca accacaggaa attgccccag
     7981 gacgctagaa atcccccttg tatacctttg tcgccctttg caaggccgta gcctgaaaga
     8041 tagatgaaga gtggcacact gaagcgcgcc cactggttca ctagtacgct taaaaaatcc
     8101 agcgagtaga agttgtggtt ttgcgcgaag cgtgtctctg actggctggt agcgtggatg
     8161 ccgacgacgg cgatcgcggc gaccaccttg aaagagtcgg tcagcgagcg ttgcatgtcg
     8221 tacgcattgg ttttgccgat gacaccgcaa acaatttcgc ttgtcccgct gccagcgatg
     8281 caggggcagg gggtatgcat gtttttatta cgggcgcagc caccggtatc ggcgaggcga
     8341 caatcgacgc gattctcgag cagatacctt cggcgggttt cacgattgct gacaagaacc
     8401 agcaggcgct gcaggaagtt tgccagaaac tgtcgcggcg cggtgtgcat aaccacggta
     8461 ttgcctgcga cctgactgac atcgacggcc ttcccgatgt ggtgaagcgc gcacgcgagc
     8521 agatgggtga ggtggagata ttggtgaaca acgccggcgt catgatcgtc gaagatctcg
     8581 cccgcatgcc gtgggaacgt ggcaaactga tgctcgatat tcattttacg gcgccgatga
     8641 agctcatcta tgaaattctg ccctcgatgc tgcagcgtgg gcagggtggt atcatcaata
     8701 tcgcgagcat ggcgggcaag gcgctgctgc ccggttgcac ctggtacggt ggatcaaaag
     8761 ccgcgatggg gcacgcttca gaaattctgt acggcgaagt gaaagacagg ggagttcacg
     8821 tgctgacggt gtatcctggc ccgatcagta ccgagctcga acgaggcgcg cgccagggtt
     8881 atgaagagaa tctggtgtcc cggttaatgc cagtcggcga acgcgaagag ctggcgcggc
     8941 gtatcgtggc ggcatacctg gcgaagcagc cggtgctcgc ttaccctgcg gtttacgacg
     9001 tcgcgcggca tctgcattcg ttttccggct ggctcgcgca ggtcttcgcc ccgcgcacta
     9061 aatcggcctg aagttatgtc acttctcaca ctgcttttt
//
LOCUS       DAOQJF010000107         9089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4063_length_9089_cov_5.277618, whole genome shotgun sequence.
ACCESSION   DAOQJF010000107 DAOQJF010000000
VERSION     DAOQJF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4063_length_9089_cov_5.277618"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLF85_07275"
     CDS             <1..746
                     /locus_tag="PLF85_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNE19502.1"
                     /translation="ILHQAGVSQDVAMHVAVGTSLAAIVFNSLRSAAAHNKRGALDWP
                     ILRRYGPFVFLGAILGAGYDSRALRLAHHIKNGIWEFDFPATQRRKKFYLRNERIEGE
                     IPHYCEVDFMKESLPEIFERSGMEKTATLVIWEGVSMYVSEDVVRNTLLACREYFGEG
                     TIVVFDYWHNTVEDPFRNLVIKVMPMVMDMIYSERFTYGATAQQMRELALSCGARRFE
                     SLSGKQVMKKLFLTSRLPLTSASMAVVEF"
     gene            816..1457
                     /locus_tag="PLF85_07280"
     CDS             816..1457
                     /locus_tag="PLF85_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19503.1"
                     /translation="MTKESLIEELKETLVTNTDLAFLAALDEATLAKVHKEIHDYIHR
                     TEEQQKPVFKVMAVATQFIPNFIIAKLAHDYLTPYIIAQVVIYMDPKAAAKIGKSLRI
                     DYMGQVALYASPEITARIGNLMEPQIVAQVVKDLSAKNFNAKLGELADLLDDKMMIEI
                     IKVLRDGRIVGNIAHAMMNQEKILRIVPQMPPEVRSGARAQLQSLGNKLAEQF"
     gene            complement(1495..1860)
                     /locus_tag="PLF85_07285"
     CDS             complement(1495..1860)
                     /locus_tag="PLF85_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19504.1"
                     /translation="MVVAPIHATKEWEVIFNEGQHWRIGIYRPKYRARDEIAELEQHT
                     CPEAFLLLKGELTMLYRDASGALHEKQLSKGELVTFSEPHAGFSANLDGVAFVVENAK
                     FETVYTDVTSGQETRRVSV"
     gene            1990..6618
                     /locus_tag="PLF85_07290"
     CDS             1990..6618
                     /locus_tag="PLF85_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE19505.1"
                     /translation="MDFPPIKENPGSPDLSTLSLNGPTTLLEIAARYAAYSAGEVDFA
                     LLAEDARRISGAAYVAINLLSKDKKATRTVALAGLGKNKKKALDLMGYAITGREWPLD
                     DFARASLKSHRLMNRGKLEGVREHFSQRMARKIQSAFGIGDIYSVGLFCQNTAIGTLV
                     FVLRKDAVIEHIADIELHAHLLGSLIYRADMENRLTVERESLRAVSENSSDVLLLIDR
                     KFRITYINRVVTGFTHKEVIGSSALDYVPADARPDYRRKLRIVFKTGKPEELELQSLG
                     ANRETAWYHVRLTPISDQPGIRYVYAVSTDITERKLHVQRLQVQADTLQQLHTFVNAT
                     SDALHVADESGRLVYCNEAACVRFGIEPRDIGKYHVWDYAPQLKGKMGWKRHLLNIKA
                     GSVQRMEGEHLNMKTGERVPVEVSTRYLSVGGRGFLVASSRDISQRRKNLEIIETQRA
                     ELQQIIAALEQSTILSITDIKGTIVRVNRHFCEISGYSEAELLGQNHRLVNSGYHDRT
                     FWRRMWKMISAGQTWRGQVRNRTKSGKFYWIETAINPIRDAQGKITHYLSIRQDITAR
                     KEAELRLIESERSLQDAQRIAKIGRWELDLVTNQLHWSQSIYDIFEINPKRFGATYEA
                     FLNAIHPEDRADVNKAYSNSLITKKPYAIEHRLLMPDGRVKWVKETCRTDYNSDGKAI
                     RSIGVLQDITDMKEAESELRFVQRVLSNTSEVARIGGWELNLLSNRLYWSDVVKQIHG
                     VKPTYQPRLDEALLFYKEGSDRDAISNAVSQVMRDGTPFDLDLRLVRADSRLLWVHAI
                     GQAEFQDGKCIRVFGTFQDIDAAKKRELQITKQNEFRRMLTEISSAFLRAQYHQLDEV
                     INAMLAQIGNHFDVDRAFLMQIDAEQMTVSNTHDYRRPGLQNHQGGVQNTPIADFPWF
                     AERIVMQDVFIIPDVSALSENAAPEKKEWREQGLHALLFVRVEIAGAPAGVLGIGSNA
                     KAVDWTDDQIDGLKLVANSLADALTRHGLEREMLTARERADEANRAKSEFLANMSHEI
                     RTPLNGVIGFTELLLNAELPATSREYVRNANTSAQTLMEVLNNILDFSKIEADKLEID
                     ESETNVAELCEQVMDVVRYSAARKGIELLLNIAQDIPRFALIDAVRMKQVLVNLLHNA
                     VKFTDHGEVELKLSASFAASTETATYQFTVRDTGIGIAPEQQHRIFEAFTQADSSISR
                     KYGGTGLGLVITSNLIEKMGGQLQLESAPGFGSTFSFALTRKCRREELLAADLSDTPG
                     SALVIDDNAASRRILKSMLEHWKITVHEAANVDEVMAIVDKVKRIGAVIVDYDLPGIN
                     GIDIVRKLKVTNKNSVQQATFVLLQTSNSDRQALLKSRILGVEHTITKPVHEHLLLDS
                     LQRLKDQATRQRATVHAATAAEPTLTPLTIGSKKILLAEDVEMNAILMKNIIRKIAPD
                     CELIIAENGAKAIQKFFAIEPDLVLMDIQMPIFDGISATHDIKSLAKGKQQSVPIIAL
                     TAGISAEERDECLRAGMNDYLTKPIDTQQLKTVLEQYLGNEPKPSAKS"
     gene            6679..6876
                     /locus_tag="PLF85_07295"
     CDS             6679..6876
                     /locus_tag="PLF85_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19506.1"
                     /translation="MQSPEQQIAEHVRAALIEAAKCAYEDAGISGLCAEGRWENALGA
                     LQTFDLQGLLKKVHPSERDGF"
     gene            complement(6910..7266)
                     /locus_tag="PLF85_07300"
     CDS             complement(6910..7266)
                     /locus_tag="PLF85_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HNE19507.1"
                     /translation="MNPILKEAFDREMSLAKQLYAAADYAGAMRRLERAHVLGQRNAW
                     PHTINHWWMLKVGLRRRDRREILGQIPRLLGGGLVSLLGRAPIGNTGGANVGIMKVMP
                     IEPELAEIFRRAGFGR"
     gene            complement(7328..7609)
                     /locus_tag="PLF85_07305"
     CDS             complement(7328..7609)
                     /locus_tag="PLF85_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE19508.1"
                     /translation="MARFCCLSWQIFCNDPPQPFLREETGITFSGYRLWLKMRGAVLE
                     LAKDTDLGRAALNGGFADQSHFNRIVRRSFGFTPTWFVQSGLQVKTFLA"
     gene            7772..9046
                     /locus_tag="PLF85_07310"
     CDS             7772..9046
                     /locus_tag="PLF85_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNE19509.1"
                     /translation="MQPSTPLAERMRPKSLADYVGQQHLVGEGAVLRRAVESKSLASL
                     ILWGPPGVGKTTLANILASELKRPFYSLSAINSGVKDIREVIDKASNGGLFTEKPVLF
                     IDEIHRFSKAQQDSLLGAVETGKITLIGATTENPSFEVISALLSRCQVYVLKPLSEAE
                     LLRLLQAALERDTFLKTKQVELAETAALFRLSGGDARRLLNVFELVIDNLVSDQAVTD
                     QVVLEIVQQNPVLYDKTGDNHYDIVSAFIKSIRGSDPNGAVYWLARMIEGGEDPSFIA
                     RRMVIAASEDIGNANPTALVVATNCFTAVNLIGYPEARIVLSQAATYLATSPKSNSAY
                     AAINAAQELVRQTGSLPVPLHLRNAPTNLMKAEGYGKGYEYSHDRPGSFSQQEYLPTD
                     IAGKKLYEPGNNAREKETREALKRLWGEKYNY"
BASE COUNT         2301 a         2481 c         2402 g         1905 t
ORIGIN      
        1 agatcctgca ccaggccggc gtctcgcagg atgtggcgat gcacgtggcg gtcggcacgt
       61 cgctggccgc gatcgtcttc aactcgctgc gctccgccgc cgcccacaac aagcgcggcg
      121 cgctcgactg gccgatcctg cgccgctacg gccccttcgt ctttctcggc gcgatcctgg
      181 gcgcgggcta cgattcacgc gcgctgcgcc tggcgcacca catcaaaaac ggtatctggg
      241 agtttgattt tccggcaaca cagcgccgca agaaattcta cctccgcaat gagcgtatcg
      301 agggcgaaat cccccactac tgcgaagtcg acttcatgaa agaatcactc cccgagattt
      361 ttgaacgttc aggcatggaa aaaaccgcca cgctcgttat ctgggaaggc gtctcgatgt
      421 atgtgagcga agacgtggtc aggaacaccc tgctcgcctg ccgcgagtat ttcggcgagg
      481 gtacaatcgt tgtcttcgac tactggcaca acacggtgga agatcccttt cgcaacctgg
      541 tgatcaaggt gatgccgatg gtcatggaca tgatctacag cgaacggttc acctatggag
      601 caacggccca gcagatgcgc gagctggcgc tgtcttgcgg ggcacgccgg tttgaaagcc
      661 tgagcggcaa acaggtgatg aaaaaacttt tcctgacttc acggcttcca ctgacttcgg
      721 cctcaatggc tgtggtggag ttctaacagg cttctgaaaa agaaatctct ggcagatttc
      781 ggaatctggc aggcttagtt aaagagagga atagcatgac taaagaatca ctgattgaag
      841 aactaaaaga aaccctggta accaacactg acctggcttt tctcgccgca ctcgatgaag
      901 ccacgctggc aaaagtccac aaggaaatac atgactatat ccaccgtacc gaagaacagc
      961 agaagccggt cttcaaggtc atggcggttg caacacagtt catacccaat tttatcatcg
     1021 cgaagctggc gcatgactac ctgacgccgt acatcattgc acaggtggtc atctacatgg
     1081 acccgaaagc cgctgcaaaa atcggcaagt cgctgcgtat cgactacatg ggccaggtgg
     1141 cgctgtatgc cagccccgaa attaccgcgc gtatcggcaa cctgatggag ccgcagatcg
     1201 tagcgcaggt agtcaaagac ctttcggcga agaacttcaa cgccaagctg ggtgaactcg
     1261 ccgacctgct cgatgacaaa atgatgattg agatcatcaa ggtgttacgc gatggccgca
     1321 tcgtcggcaa catcgcgcac gccatgatga accaggaaaa aatactgcgc attgtaccac
     1381 agatgccgcc cgaagtgcgc agcggcgcac gcgcgcaatt gcagtcactc ggcaacaagc
     1441 tggcagaaca gttttgaaaa tcgattcggg tggcttagtc acccgaatca gcgctcagac
     1501 agaaacccgg cgagtctcct gacctgacgt aacgtccgtg tacacggtct cgaattttgc
     1561 attctcgacg acaaaagcca caccgtcgag gttggctgaa aaaccggcgt gcggctcgct
     1621 gaaggttacg agctccccct tgctgagctg cttctcgtgc aaggcgcctg acgcgtcacg
     1681 atagagcatg gtgagctcgc cctttagcag caggaatgcc tcagggcagg tgtgctgttc
     1741 gagctcggcg atttcatcgc gggcacggta tttaggccga tagatgccaa tacgccagtg
     1801 ctggccctcg ttgaagatta cctcccattc tttggtggca tgaatcggag cgacaaccat
     1861 acttgattct cggtttgtct gttcccgggc aaagcggaaa ataacaggat ctgggccagg
     1921 aaataaatgg atagaatatt ccataattta tagtttacga gctgtctttt acacgaggcc
     1981 tgcaagttca tggattttcc tcctataaaa gaaaatccgg gtagccctga cctgtctact
     2041 ctctcgctca acggaccgac tacactactc gagatcgcgg cacgctatgc ggcatactct
     2101 gccggtgagg tagatttcgc gctgcttgct gaagacgccc gcaggatttc tggcgctgca
     2161 tatgtcgcca tcaacctgct cagcaaagat aagaaagcta cgcgcaccgt agcgctcgcc
     2221 ggcctgggta agaataagaa gaaagcactc gacctgatgg ggtatgcgat caccggccgc
     2281 gaatggccgc tcgacgactt tgcccgcgcg tcgctaaaga gccaccgact gatgaaccgg
     2341 ggaaaactcg agggtgtgcg cgaacatttc agccagcgta tggcccgcaa gattcaatcg
     2401 gcctttggca tcggcgacat ctatagcgtc ggcctgttct gccagaatac cgcaatcggc
     2461 acactggtct tcgtactgag aaaagatgcc gtcatcgaac atattgcaga catcgaacta
     2521 cacgcgcacc tgctgggcag cctgatctac cgggccgaca tggaaaacag actgacggta
     2581 gagcgggagt cgttgcgtgc ggtttctgaa aattcgagcg acgtacttct gcttatcgac
     2641 cgcaaatttc gtataaccta catcaaccgt gttgtgacgg ggttcacaca taaagaggtc
     2701 atcggcagca gcgcactcga ctatgtaccg gctgatgccc ggcccgatta ccggcgcaag
     2761 ttgcgcattg tcttcaagac tggcaaacct gaagaactgg aactacagtc tctgggcgca
     2821 aaccgggaaa ctgcatggta tcatgtccgg ctcaccccca tcagcgacca gccaggcatc
     2881 aggtatgttt atgcggtcag tacagatatc accgagcgaa aactgcatgt gcagcgcctg
     2941 caggtacagg ccgatacgct acagcagctg catacgtttg tcaacgcgac cagcgacgcg
     3001 ctgcatgttg ccgatgaaag tgggcgactg gtatactgca acgaagctgc ctgtgtacgc
     3061 ttcggcatcg aaccgcgaga tatcggaaag taccacgtat gggattacgc tccgcagttg
     3121 aagggaaaga tgggctggaa acggcacctg ctgaatatca aggcaggctc ggtacagcgc
     3181 atggaaggcg aacaccttaa catgaagaca ggtgagcgtg tgccggtcga agtttctacc
     3241 cgctacctga gcgtcggtgg ccggggcttt cttgtcgcca gctcgcgcga tatttcgcag
     3301 cgcaggaaaa acctcgaaat cattgaaacc cagcgcgccg aactgcagca gattatagct
     3361 gcgctggaac aaagtaccat tctttccatt acagacatca agggtactat tgtccgggtc
     3421 aatcgacact tctgtgaaat ttccggctat tccgaagccg aactgctcgg tcagaatcac
     3481 cgtctggtca attcgggata ccacgaccgc actttctggc gccgcatgtg gaagatgatt
     3541 tccgcaggcc agacatggcg gggacaggtt cgcaatcgaa caaaaagcgg caaattctac
     3601 tggatcgaaa ccgcgattaa cccgattcgc gacgcgcagg gaaagatcac gcactacctg
     3661 tctatcagac aggatatcac agcccgcaaa gaagccgaat tacggctgat cgaaagcgag
     3721 cgcagcctgc aggacgcaca acgcattgcc aaaattggca ggtgggagct agacctggtc
     3781 accaaccaat tacactggtc acagtctatt tacgatattt tcgaaattaa cccgaaacgt
     3841 tttggtgcaa cgtacgaagc tttcctaaac gctatacacc ctgaagacag ggctgatgtc
     3901 aataaggctt acagtaattc tctcatcaca aagaagcctt acgcaattga acaccgcctg
     3961 ctgatgccag acgggcgtgt taaatgggtc aaagagacct gccgcacaga ctataactct
     4021 gatggcaaag caatccgctc catcggcgtt ctgcaggata ttacggatat gaaagaggca
     4081 gagtctgaac tccgcttcgt gcagcgcgtc ttgtcgaata ccagcgaagt ggcacgcata
     4141 ggtggttggg agctcaatct gctgagcaac cggctttact ggtcggacgt tgtgaagcag
     4201 atccacggcg tgaaaccgac gtaccagccc aggctcgatg aggcactttt gttttataaa
     4261 gagggctccg atcgcgacgc aataagcaat gcggtttcgc aggtaatgcg cgacggcacg
     4321 ccgttcgatc tggacctgcg cctcgtcaga gccgattcgc gcttactctg ggttcacgca
     4381 atcggccagg ccgaattcca ggatggcaag tgcattcggg tctttggcac ctttcaggat
     4441 atcgatgccg ccaagaaaag agaactccag atcaccaaac agaacgaatt ccgccggatg
     4501 ctaacagaaa tctcttctgc ctttctgcgc gcacaatacc accagctcga cgaagttatc
     4561 aatgctatgc tcgcccagat tggtaatcac ttcgacgtcg accgggcttt cctgatgcag
     4621 atcgatgctg agcagatgac agtttcaaat actcatgatt accgtcgtcc gggactgcaa
     4681 aaccaccaag gcggcgtgca aaatacgccg atcgccgatt ttccctggtt tgcggaaaga
     4741 atcgtcatgc aggatgtttt tatcataccc gatgtcagtg cgctctcaga gaatgctgcc
     4801 cctgaaaaga aagaatggcg tgaacagggc ctgcatgcgc tgctctttgt ccgtgtcgaa
     4861 attgccgggg cacccgcagg tgtcctggga attggcagca acgcgaaagc cgttgactgg
     4921 accgacgacc agatcgacgg tcttaagctg gtggcaaaca gcctggccga tgcactgacg
     4981 cggcatgggc tcgagcgcga aatgttgact gcacgcgagc gcgccgacga agccaatcgg
     5041 gccaagtcgg agtttcttgc caatatgagc catgaaatcc gtactccgct gaacggcgtg
     5101 attggtttta cagaactttt gctgaatgcc gaacttcctg caacgtcgcg tgaatatgtc
     5161 cgcaatgcca atacctcagc gcagacattg atggaagtgc tgaacaacat cctggatttt
     5221 tccaaaattg aagcagataa actggaaata gatgaatcag agaccaatgt cgcggaactc
     5281 tgcgaacagg taatggatgt cgttcgctat tctgcagccc gaaaaggtat cgaactgcta
     5341 ctcaatatcg cgcaggatat tccgcgtttt gcgctgatcg acgcagtgcg gatgaagcaa
     5401 gttcttgtca atctgctgca caacgcggtt aaatttaccg accacggtga ggtcgaactg
     5461 aagctttctg cgtcttttgc tgcaagcaca gaaacagcga cataccaatt caccgtccgt
     5521 gacaccggca ttggaatagc ccccgaacaa cagcaccgga tcttcgaggc tttcacccag
     5581 gctgactcat caatctcccg caagtacggt ggcacgggac ttggtctcgt tattaccagt
     5641 aatctcatcg aaaagatggg cggccaactg cagctggaaa gcgcaccagg tttcggcagt
     5701 acattcagct tcgccctgac gagaaaatgc cggcgtgaag agctgctagc tgccgacctg
     5761 agtgatactc caggctcggc actcgtcatc gatgacaatg cagccagccg ccgcatactc
     5821 aagagtatgt tggagcattg gaaaattacc gttcacgaag cggcaaacgt cgacgaagtg
     5881 atggcaatcg tcgacaaggt aaagcgtata ggagcggtta tcgtcgacta tgaccttccg
     5941 ggcatcaatg gcattgacat cgtgcgcaag ctcaaggtca ccaacaaaaa cagcgtgcaa
     6001 caggccacct tcgtactgct gcagacttca aactctgacc gacaggcact attaaagagc
     6061 cgcattctgg gggtagaaca cactatcacc aaacccgtgc acgaacacct tctgctcgat
     6121 agcctgcagc gcctgaagga ccaggcgacc cggcagcgcg caaccgtaca cgccgcgaca
     6181 gcagcggaac caacgctcac accgctgaca atcggcagca aaaagatact gctcgccgaa
     6241 gacgttgaaa tgaacgcgat cctgatgaag aatataatcc ggaaaatcgc tcccgattgc
     6301 gaactgatta tcgctgaaaa cggtgcaaaa gccatacaga aattcttcgc cattgaaccc
     6361 gacctggtgc tcatggatat acagatgccg atttttgatg gaatttctgc cacccacgat
     6421 attaaatccc tggcgaaggg aaagcaacag tctgtaccca taattgcact cacggctggt
     6481 atttcagccg aagaacgtga tgaatgtctg cgagcaggca tgaacgacta tctcacgaaa
     6541 ccgatcgata cgcagcagct gaaaacagtg ctggagcagt atctcggaaa cgagccgaaa
     6601 ccttctgcca aaagctgaat gccataatct gcacgaatat tcgtctttac gcgttgcggc
     6661 ggcaggcagc gtgacaacat gcagagtccc gaacaacaaa tcgccgaaca tgttcgcgct
     6721 gccctgatcg aggccgcaaa gtgtgcctac gaagatgcag gcatcagcgg cctgtgtgct
     6781 gaaggccggt gggaaaacgc actcggcgcg ttgcagacct ttgatcttca gggcctgcta
     6841 aagaaggtgc atccgtcgga gcgcgatggt ttttaatctg gcgctgcgtc acggaagcaa
     6901 cagaagctgt taacgcccga acccggcccg gcggaaaatc tctgccagct cgggctcaat
     6961 aggcataact ttcataattc ccacattagc gccacctgta ttgccaatcg gcgcacggcc
     7021 cagcaacgag acaagcccgc cgcctaatag ccggggtatt tgacctaaaa tttcccgtct
     7081 atcgcgccgg cgcaggccca ctttgagcat ccaccaatgg tttatcgtat gcggccaggc
     7141 attgcgctgg cctaaaacat gtgcccgctc gagcctgcgc atggcgccgg cgtagtctgc
     7201 tgcagcataa agttgtttgg ccaggctcat ttccctgtcg aatgcttctt tgagtattgg
     7261 gttcatgctt cctccatgaa tgccgccaaa gtaccctatg cgcgactgtt cgccttgaac
     7321 aaagcggcta agcaagaaaa gtttttacct gcaggcctga ctgcacaaac caggtgggag
     7381 taaaaccaaa actccgcctc acgatgcggt tgaagtgcga ctggtcggcg aaaccaccgt
     7441 taagtgcagc ccggcccagg tcagtatctt ttgccagctc aagaactgcc ccacgcattt
     7501 ttaaccaaag ccggtagccc gaaaaggtaa ttccggtctc ttcgcgtaaa aatggctgtg
     7561 gcgggtcgtt gcaaaagatt tgccacgata gacagcaaaa tcgggccata aaaacaggct
     7621 ggacataggt gctcggtacg ggagacttcg cacgagtcat ttgtaaaccg gttttgaagc
     7681 gtttaggaac ttttgtcagc caagcacctt actctgtgaa tgctctttac agccctcttg
     7741 acaagtccac acagcaccga ttagttagaa aatgcaacca tcaacgccac ttgccgaacg
     7801 gatgcgcccg aaatcgctcg ccgactatgt tggccagcag cacctcgtgg gtgaaggtgc
     7861 agtactgcgc cgcgccgtag aatcaaaaag cctggcatcg ctgattctct ggggtccgcc
     7921 gggtgtcggc aagaccacac ttgcgaatat tctcgcaagc gaactcaagc gacctttcta
     7981 cagcttaagc gccatcaact cgggtgtgaa agatattcgc gaggtgatcg acaaagcctc
     8041 aaacggcggt ctcttcacag aaaagccggt gctcttcatc gacgagattc atcgtttctc
     8101 taaggcgcag caagattcgc ttttgggcgc ggttgaaacc ggcaagataa cgctcatcgg
     8161 cgctacgaca gaaaatccct cttttgaagt catctctgcc ctgttgtcac gctgccaggt
     8221 ctatgtgctc aaacctttgt ctgaggcgga gcttctgcgt ttattgcagg cagcactcga
     8281 aagggataca ttcctcaaaa cgaaacaggt ggaactggcc gagaccgcag ccctgttccg
     8341 tttgtcaggc ggtgatgcgc gccggctgct gaacgtattc gaactggtaa tagacaacct
     8401 ggtcagtgac caagcggtca ctgaccaggt tgtccttgaa atcgtgcagc aaaacccggt
     8461 gctgtatgac aagacgggcg ataaccatta cgacatagtg tctgccttta tcaagtcgat
     8521 acgtggcagc gacccaaatg gtgctgtcta ttggctcgcg cgcatgatcg aaggagggga
     8581 agatccctct tttatcgccc ggcgcatggt gattgccgct tcggaagata tcggtaacgc
     8641 gaacccgacc gcgctcgtcg ttgccaccaa ctgttttacc gcagtcaacc tgatcggtta
     8701 tcccgaggcg cgcattgttt tatcgcaggc ggcgacttat ctcgcgacct cgcccaagag
     8761 taattcagcc tacgcggcaa tcaatgcagc acaagagctc gtacggcaaa cgggcagttt
     8821 accggtgccg ctgcacctgc gcaatgcgcc gacaaacctg atgaaggctg aaggttacgg
     8881 caagggttac gaatactcac acgaccgacc aggcagtttt tcccaacagg aatatctgcc
     8941 tactgacatt gctggcaaga agctgtacga accgggtaac aacgcgcgtg aaaaggagac
     9001 gcgtgaggcg ctcaagagat tatggggcga aaagtacaat tattaacagc actctgcggt
     9061 acagtggttt tgttcagtcg gtgcgtggc
//
LOCUS       DAOQJF010000108         9048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4096_length_9048_cov_4.553319, whole genome shotgun sequence.
ACCESSION   DAOQJF010000108 DAOQJF010000000
VERSION     DAOQJF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4096_length_9048_cov_4.553319"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PLF85_07315"
     CDS             complement(<1..1332)
                     /locus_tag="PLF85_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE19510.1"
                     /translation="MLPDYYQFSLPTKVKYGIGLASRLGDELADFPGSRALLVTDKVI
                     QSTGLAKKVIEGLKGSKIEIVATYDNVPPNSEIKVVTEAAELGVANKCDMVIAIGGGS
                     VLDSAKVINLLMVKGGPLQKHMGAQLLKDKLLPYVAIPTTSGTGSEATQFAVISDDAN
                     SVKLPFAEDFFLPDVAILDPEMTATMPGKVTAATGMDALTHAIECYAGMNPNPVSEAL
                     ALYAIELIAQNILQAVAVPNDLNARGAMLTASFIAGVAFNHSGVGIVHGISHALGGVY
                     HIPHGLANSIILPFSVEYNMETSAARYARVAQIFGDSGFYPVKELNQFVARFDNFAVN
                     QAVTQLGLVDEWLTKQRAQSLAGKLRAMNQKLNALCGHPLSLREAGVKDNLAKLEQVV
                     RTAMEDGAMLNNPVPATADGVRAIVKEAFSQDLKPIAVSKSELKAARTSGAA"
     gene            1473..2651
                     /locus_tag="PLF85_07320"
     CDS             1473..2651
                     /locus_tag="PLF85_07320"
                     /inference="COORDINATES: protein motif:HMM:NF022738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HNE19511.1"
                     /translation="MAQIPLDTFYRHLTPADSLATAFDDKNTVPLPDDWYIVITDIVG
                     STAAIGAGRYKDVNTAGGLAAMALSNAFGTLEFPFIFGGDGTTFLLPPEIRDAVAEIL
                     ADTARKVKEFFSLDLRVGIVPAREVYAKKQNIRVGKIRVSPHYVQAVITGDGIDYAER
                     LVKTGTTYLVPHELTGTHEANFTGFTCRWHDIPSAGGETVALIVRFSAGGEKINMQLL
                     QTVLAAIERHYGPDAQHHPIKEDTLRNTLSPKIIWREAAAKAGKRGGVLFVLQMIRIA
                     FESLVTLLAIRLHLPLRVFHYKLNRVKWYNVISSDYKKFDGMLKMVISGSPAARAGLV
                     RELDRLKAENKLQYGIHVSDRALLTCLLHADSAQEVHFVDAADGGYALAAKMLKEQIH
                     "
     gene            2662..5850
                     /locus_tag="PLF85_07325"
     CDS             2662..5850
                     /locus_tag="PLF85_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE19512.1"
                     /translation="MNLIATSILRPVFAWILMSGLIIFGAIAASRLGVSQLPDIDFPI
                     LTIQVTYEGATPEVVESALIMPIEERLLGIEGLKEMRATARQGSGQIVLELDIRRNVD
                     VALQEVQAALSQLRLPPGIDPPVIRKTNPEDQPFIFMGVYADKPLKEVLRWIDIFLLD
                     QFRFIPGIGEASIQGFPERNLRIWPDLAKMRNLDLTVQDLITAVETQHLESTAGQFVR
                     QKDELRVRWLGEAQSPEDLARVRILRRGNENIFTTGKPLTIGDVARVEDGLSDERRMA
                     RIEGRQAITIGIRKQRGQNEVALAEAVHQKVAELQSQLPESYNLKIIVDMSRPTRAVV
                     NTTWEKLAFAVVVTILVCLAFLGNWQAAVNIIFSIPTSIVGTFLIVYFGNFTLNLFTL
                     LALTLAVSIVVDDAIMLLENIVRHHRMGKSAAQAAYDGSMEILPAASAATLAVIAVFL
                     PVVVMDGVIGKFFFQFGVVMSAAVLLSLVEAVTITPMRSAAILSDNPQRNKAELWLEE
                     LFERIAERYRTSLQWVLRHPVKITSVSAVLFALSLLLIFRVRQEFVPMQDQDYIFISG
                     QAKPGASLEATRARALAVETIVRQHPDVSDYLLSVGAGGAAAEINQFFMPVKLKPRAT
                     RKKNHRAIMEDLRRGFKAIEGARISLRDFSNRGLTSGRQFPVSFNIFGPDLDVLDEKV
                     QQIIQRLNSEGLTQDLDTDFKRNFPELQIQPDRAAMAARSVSIQSVATTLGATVAGLR
                     EGRYTADGRRFDIRVKLPDEKISSASDIGQILVRNAAGNTIPLSQLVVFKETKTYQAI
                     NRVNRQRSIGVFGNLQSGKSQSAVLSRAKQVSQEILPPGYSFALEGGAAGLAESFRSL
                     NLALFLGILVAYMILAVQFNSFVHPVSVLIALPFSVTGALISLWLADVSLNLFSFIGL
                     IVLMGISKKNSILLVEFTNHVRQHEHDDVRHALVAACPVRLRPVLMTSAATVFAALPL
                     VIGNSVGQETRTPIGLTIVGGTIVSTFFTLYVVPCFYYLLSKLERPQKQLLTIHEPFR
                     GSSATQPMVVESVAAPEDKEARPARTRRRGSG"
     gene            complement(5842..6255)
                     /locus_tag="PLF85_07330"
     CDS             complement(5842..6255)
                     /locus_tag="PLF85_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19513.1"
                     /translation="MALTDSQKSELIRRGNELFNQGRFFEAGRVYTTVGYKTGLIRLG
                     DLFYFEKEMPLVAYGFYRNANHKPMLDKIGDGFVFALKCLLDDGPAQPKPQKNVADEQ
                     LKVRQANSPDDTTPVGQETVDRKIERAVAMLKRLA"
     gene            complement(6314..>9048)
                     /locus_tag="PLF85_07335"
     CDS             complement(6314..>9048)
                     /locus_tag="PLF85_07335"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19514.1"
                     /translation="TFRIRLKEKTAAARALRKAVQVREKSLGNTWRSFVKTFGARVQY
                     SYQTRRFDTYYSTYLTEKNLKDFENQLLSAVAASLPRAPDKVVFEGWKKTAARVAEAE
                     IAAALLEENLSGKRDEQELERKRAQIAKNRTLLMSDLSRVFDPYLQAKLDRMQFERQT
                     IRILSYGTELENLSAEVEAGKGGKKLKMPGPLLDTGMRAVADTAESWALLRSGEFSDY
                     VRRVFTEPDEYFHSTGELGGKRGREIRLARSSLEVRFAPVPMQLPVVERARLASAAEK
                     DAPDFLQKFIAEDSRFAAELKLLAAKKSQRLDVLREKGTPPYKDADYMAAAREYRALV
                     AQRDAALREIVHFAALEKEQATQKLARLKERETVLAQTTKQIKELTALLQLAAKGKAP
                     KETPSVSELERSIELARQQSEAERGEIAALKAQAGSYEPGAADGAADRERAQKLMLAE
                     ALLNLRDAALYGRIRLALVSEHDLLRQQRRNPAARNEQLRHFAAVRQFINEAKNETDI
                     PVARGATSPLAGGVLAITRSEAEEQMHDLDTRPLLGDKGLVRILLNENIAGYNMIVVD
                     KTSGLARIWNSTQRLLWFSSGIALLAVLAAWYFSGLAVRRIASLSSTSQQVRNGNLNV
                     SFADRGYDELSSLGQSLNSMVEGLREREELRGELMAAEEIQKRLLPASVPVNLKGRAD
                     IAGFYKAMVGIGGDYFDYVPIGSDYIAIAMGDVSNHGVGPALVMAITRSQLHAELRAK
                     ELSVRSILLKLNEQLYAETPANIFVTFFLALYNLKTGEMQYASAGHSKPLLYRAAGGK
                     TSYLEAGGMPLGMDDNDFFADTLEIRKVKLEPGDIFVQYTDGLSEAMNAAREQFGYDR
                     MSEQLVRNAAQPAEGVLTGLSRAVEQFTGTRLDQPGPSALNDDIALVCLRRTAV"
BASE COUNT         1901 a         2668 c         2500 g         1979 t
ORIGIN      
        1 tgctgcaccc gaagtgcgtg cggctttgag ctctgacttg gaaaccgcaa tcggcttcag
       61 gtcttgcgag aacgcttctt tgacgatcgc ccgaacacca tcggccgttg ccggtaccgg
      121 gttgttgagc attgcgccgt cttccatcgc tgtgcgcact acctgctcaa gcttcgccag
      181 gttgtctttg acaccggctt cgcgcagcga caggggatgc ccgcagagcg cgttcaactt
      241 ttggttcatc gcgcgcagtt tgcccgcgag cgattgcgcg cgctgtttag tcagccactc
      301 gtcgacgagg cccagctgcg tgacagcctg gttcaccgcg aagttgtcga accgggcaac
      361 aaactggttc aactctttga caggatagaa gccgctgtcg ccgaagatct gcgcgacgcg
      421 cgcataacgc gcggccgagg tttccatgtt gtattcgacg ctgaacggca ggatgatact
      481 gttcgcgagg ccgtgcggta tgtggtaaac tccaccgagt gcatggctga taccgtgaac
      541 gatacccaca ccgctgtggt tgaatgccac accggcaata aaagacgcag tcagcatcgc
      601 accgcgcgcg ttcaggtcat tcggcacggc aactgcctgc agaatgttct gtgcaatcag
      661 ttcgatggcg tagagagcga gcgcctcaga aaccgggttc gggttcatgc cggcgtagca
      721 ctcgatcgcg tgcgtcagtg catccatacc ggttgcagca gttaccttgc cgggcatggt
      781 ggccgtcatt tcggggtcga ggattgccac gtcgggcagg aagaagtctt cagcaaaggg
      841 cagcttcacc gagttcgcat cgtcagaaat aacggcgaat tgggttgcct cggaaccggt
      901 accagaagta gtcggtatag ccacgtaagg cagcaattta tctttgagaa gttgagctcc
      961 catgtgcttc tgcaaggggc cgcctttaac catcagcagg ttgattacct tggctgagtc
     1021 gagcaccgaa ccaccgccga tggcgatgac catgtcgcat ttgttcgcaa caccgagttc
     1081 tgcggcttcg gtcacaacct tgatttcact gttgggtgga acattgtcgt aggtcgcaac
     1141 aatttcaatc ttcgagccct tgaggccttc aatgaccttt ttggcaagtc cagtcgactg
     1201 gatcaccttg tcggtgacga gtagcgcgcg gctgccggga aaatcggcaa gttcatcgcc
     1261 gagacgcgat gctaggccga tgccgtattt gactttggtc ggcagcgaaa actggtaata
     1321 atcaggtaac atgcggtatt tatttgcccc gaagtgaggg cgacaagaat taaggtgaac
     1381 aaacgctcaa ttattaaggc tcgaggacaa ccgctgccgg gtgggatcaa aaatcctcgt
     1441 gaaagtgcgt ctcagtggta tatattgtcc caatggcgca gatacccctc gatactttct
     1501 atcgccacct gacgccggcc gattcactcg caacggcatt tgatgacaag aacactgtcc
     1561 ccctgcccga cgactggtac atcgtcatta cggatatagt aggttcaacg gccgcgatcg
     1621 gggccggtcg ctacaaagac gtcaacacgg cgggcgggct tgccgcaatg gcgctcagca
     1681 atgcctttgg cacgctggag tttcccttta tctttggcgg cgacggtaca acattcctgc
     1741 tacctccgga gatacgcgac gcggtagccg agatcctggc agacactgcc cgcaaggtga
     1801 aagagttctt ttctcttgac ctgcgtgtgg gcatagttcc cgcacgcgag gtctacgcaa
     1861 agaaacagaa catcagagtc ggcaagatca gggtttcgcc gcactacgtg caggcagtca
     1921 tcacgggcga cggcatcgac tacgccgaga gactcgttaa aaccggcacg acctaccttg
     1981 tgccgcacga gctaaccggc acccacgagg ccaacttcac cggctttacc tgccgctggc
     2041 acgacatacc gagcgcgggc ggcgaaacgg tagcactgat tgtgcgtttt agcgcaggcg
     2101 gcgaaaagat caatatgcag ctgttgcaga ctgtgctggc ggcaatcgag cggcactatg
     2161 gccctgatgc ccagcaccac ccgattaaag aagacacact gcgcaacacc ctttcgccaa
     2221 aaattatctg gcgcgaagcg gcggccaaag caggcaaacg cggtggagtg ctctttgtcc
     2281 tgcagatgat acgcattgcc tttgaatcgc tcgtaactct gctcgcgatc aggctgcacc
     2341 tgccgctcag ggtctttcac tataagttga accgcgtgaa atggtacaac gtcatctctt
     2401 ctgactacaa aaaatttgat ggcatgctga agatggtcat cagcggttcg cccgcggcgc
     2461 gcgcaggcct cgtgcgcgag ctcgaccggc tgaaggcaga aaacaagctg caatacggta
     2521 tacacgtttc agaccgggcg cttttgacct gcctgctgca cgccgacagc gcgcaggaag
     2581 tccatttcgt cgatgctgcc gacggcggtt atgcgctggc agcgaagatg ctcaaagaac
     2641 agattcactg aaatttctcc gatgaacctg atagccacct caatactacg ccccgtcttc
     2701 gcctggattt tgatgtcggg cctgattatt ttcggcgcaa tcgcagcgtc gcgcctcggc
     2761 gtcagccagc tgcccgacat tgattttccc atactcacga tacaggtaac atacgagggg
     2821 gcgacccccg aagttgtcga atcggcgctc atcatgccga tcgaagagcg cctgctcggt
     2881 atcgaaggcc tcaaagagat gcgcgccact gcccggcagg gttcgggaca gatcgtgctc
     2941 gagctcgaca tccgccggaa cgtcgacgtc gcgctgcaag aggtgcaggc agcgctcagc
     3001 cagctgcgcc tgccaccagg tatagacccg ccggtgattc gcaagaccaa ccccgaagac
     3061 cagccgttca tcttcatggg cgtctacgcc gacaagccgc tcaaagaggt actgcggtgg
     3121 atcgatatct tcttgctcga ccagttccgt tttattcccg gcatcggcga ggcgtcgata
     3181 cagggttttc ccgagcgcaa cctgcgcatc tggcctgacc ttgcaaaaat gcgcaatctc
     3241 gacctcactg tgcaggacct catcactgct gtagagacgc agcacctcga gagcactgca
     3301 ggccagtttg tcaggcagaa ggatgaactg cgcgtgcgct ggctcggtga agcacagtcg
     3361 cccgaagacc tcgcgcgcgt gcgcatactg cggcgcggca acgaaaatat ttttaccacc
     3421 ggaaaacccc tgaccattgg cgatgtcgcg cgtgtagaag acggactcag cgacgagagg
     3481 cgcatggcgc gcatcgaagg ccgccaggct atcacgatcg gtatacgcaa gcaacgcggg
     3541 caaaacgagg tggcactggc cgaagcggtt caccagaaag tggcggaact gcaatcgcag
     3601 ctgcccgaga gttacaacct caagatcatc gtcgatatgt cgcgcccgac gcgggccgtc
     3661 gtcaacacca cctgggaaaa actcgcgttc gcggtcgtag tcacaattct cgtctgcctc
     3721 gcattcctgg gcaactggca agcggcggtg aatatcattt tctcgatacc gacctcgatc
     3781 gtcggcactt ttctcatcgt ctatttcggc aacttcacgc tcaacctgtt caccctgctg
     3841 gcgctgacgc tcgcggtttc gattgtcgtc gatgacgcga tcatgcttct cgagaatatc
     3901 gtgcgccacc accgtatggg caagtctgcg gcgcaggctg cctatgacgg ttcgatggaa
     3961 attttgcccg cggcgagcgc ggcgacgctg gctgttatcg cggtattttt accagtcgtc
     4021 gtcatggatg gcgtgattgg caaattcttc ttccagtttg gcgtcgttat gtccgcggcc
     4081 gtcctgctgt cgctggtcga ggcagtgacg attacgccga tgcgctccgc agcgatattg
     4141 tccgataacc ctcagcgtaa caaggcggag ctctggcttg aagagctttt tgaacgtatt
     4201 gcagaacgtt accgtacttc gctgcaatgg gttttgcgcc acccggttaa aattacctct
     4261 gtttccgcag tactctttgc gctttcgctg ctgctgatct tccgcgtcag gcaggaattt
     4321 gtcccgatgc aggatcagga ctacatcttt atttccggcc aggcaaaacc tggtgcgtca
     4381 ctcgaagcaa cccgcgcccg cgcgcttgcg gttgaaacca ttgtgcgcca gcacccggat
     4441 gttagcgact acctcttgtc agtcggcgca ggcggggccg ccgccgagat caaccagttc
     4501 ttcatgccgg taaaactcaa accccgcgcg acgcgcaaaa agaaccaccg ggccatcatg
     4561 gaagatctgc ggcgcgggtt caaagcaatt gaaggtgcgc gaatctcgct acgcgatttc
     4621 tcgaaccgtg gtctcacatc gggcaggcag ttcccggtat cgttcaacat cttcgggccc
     4681 gacctcgatg tactcgacga gaaggtacaa cagataatcc agcgcctgaa ctccgagggc
     4741 ctgacgcagg acctcgatac cgatttcaaa cgcaactttc ctgaactgca gatccagccc
     4801 gaccgggccg ccatggcagc gcgctcggta tccatacaat cggtcgcgac aacgctcggc
     4861 gcgactgtcg ccggcctgcg cgaaggtcgt tatacggcag acggcaggcg ttttgatatc
     4921 cgcgtaaagc tgcccgacga gaaaatttcg tcagcgagcg acatcgggca gatactggtg
     4981 cgcaacgctg ccgggaatac cataccgctg tcacagctgg tggtatttaa ggagacaaaa
     5041 acctaccagg cgatcaaccg cgtcaaccgg cagcggtcga tcggcgtctt tgggaacctg
     5101 cagtcgggca agtcacagtc tgcggtactc tcgagggcaa aacaggtctc gcaggaaatt
     5161 ttgccgcccg gttattcgtt cgcgctcgaa ggcggagctg cgggactcgc agaatcattc
     5221 cgcagcctga atcttgcgct ctttttaggc atcctggtcg cgtacatgat actagccgtg
     5281 cagtttaatt cctttgtgca cccggtttcg gtgctgattg cgctgccatt cagtgtcact
     5341 ggagcgttaa tttccctgtg gctggccgac gtctcgctga acctgttcag tttcatcggt
     5401 ctcattgtgc tcatgggcat ctcgaagaag aactcgatac ttctggtaga atttaccaat
     5461 catgtgcgcc agcacgagca cgacgatgtg cgccatgcgc tcgtggcagc atgccctgtc
     5521 aggctccgcc ccgtactcat gacctctgcc gcgaccgtct ttgccgcgct tccgctggtt
     5581 atcggcaaca gcgtcggcca ggaaacgcgt acccctatcg ggctcaccat cgtcggtggc
     5641 acgatcgtct ccacattctt cacgctctac gtggtcccgt gtttttatta cctgttgagt
     5701 aaactcgaaa gaccgcagaa acaattgcta acaatccatg agccattccg tggttcgtct
     5761 gcaacgcagc ctatggtagt agaatctgta gctgcaccag aagataaaga agccaggcct
     5821 gcacggacca ggcgccgcgg ctcaggctaa acgcttgagc atcgcgacag cacgttcaat
     5881 cttgcggtcg accgtttcct ggcccaccgg tgtggtatcg tcgggcgaat ttgcctgccg
     5941 aaccttcagc tgttcgtcag ccacattctt ttgcggctta ggctgcgcag gtccatcgtc
     6001 gagcaggcat ttgagggcga acacaaagcc atcgccgatc ttgtcgagca tgggcttgtg
     6061 attggcattg cggtaaaagc cgtaggcgac caggggcatt tccttttcaa aataaaagag
     6121 atctcccagc ctgatcagac cggttttata gcctacagtt gtgtagaccc ggccggcctc
     6181 aaaaaaacgc ccctgattga acagttcatt accccggcga atgagttcgg atttctgcga
     6241 gtcagtcagc gccatgttaa agatccctgg ctttcttatc ggccgtatgt atacttttgc
     6301 atagcaattc gtctcacaca gctgtccgcc gcaggcagac gagagctata tcatcgttca
     6361 gcgcacttgg cccgggctgg tcaaggcgcg tgcctgtgaa ctgctccacc gcacgcgaca
     6421 ggcctgtcag cacgccttca gcaggctgcg cggcattacg caccagctgc tcgctcatac
     6481 ggtcgtaacc aaattgttca cgcgcggcat tcatggcttc gctgagtcca tcggtgtact
     6541 gcacaaagat atcgcctggc tctagcttca ctttgcggat ttcgagcgtg tcagcgaaga
     6601 agtcgttgtc gtccataccg agcggcatac cgccggcttc gagatagctc gtcttgccgc
     6661 ccgccgcacg gtacaataat ggttttgagt gccccgccga agcatactgc atctcgccgg
     6721 tcttgagatt gtagagcgcg aggaaaaacg tgacaaaaat attcgccgga gtttccgcgt
     6781 agagttgttc gttgagtttg agcaggatac tgcgcacaga aagttctttc gcacgcagtt
     6841 cggcgtgcag ctgcgagcgc gtaatggcca ttacaagcgc aggaccaacg ccgtggtttg
     6901 agacatcacc catcgcgatg gcaatatagt cgctgccgat gggcacgtag tcgaagtagt
     6961 caccgccgat accgaccatt gccttgtaga acccggcaat atctgccctt cccttcaggt
     7021 tgaccggcac cgaagccggc agcagacgtt tctgaatttc ttctgccgcc attagttcac
     7081 cgcgcagttc ttcacgctcg cgcaggccct cgaccatgct gttcaggctc tgcccgagag
     7141 aggaaagttc atcgtacccc ctgtcggcaa aagacacgtt caggttacca ttgcgtacct
     7201 gctgcgaggt actgctgagc gaggcgatgc gcctgaccgc gagaccagaa aaataccagg
     7261 cggccaggac cgcgagcagc gcaatacccg aagagaacca gagcaaccgc tgcgttgaat
     7321 tccagatacg cgccaggccg gatgtcttgt cgacaacgat catattatag cccgcgatat
     7381 tctcgttcaa cagaatgcgt accaggcctt tgtcgcccag cagcggccgc gtatccaggt
     7441 catgcatctg ctcttcggct tcagaacggg taattgccag aactccaccg gcgagcggtg
     7501 aggtggcacc acgcgctaca ggaatatcgg tttcattctt cgcctcgtta atgaactggc
     7561 gcaccgccgc gaagtgtctc agctgctcat tgcgcgccgc cgggttgcgg cgctgttgac
     7621 gcagcaggtc atgttcagag accagggcaa gtcgaatgcg cccgtagagc gccgcatcgc
     7681 gcaggttcaa caaagcttca gcgagcatca gtttttgcgc gcgttcgcgg tcagcagcgc
     7741 catcagccgc cccgggttca taagagcccg cctgcgcttt cagggctgcg atttccccgc
     7801 gctcagcttc tgactgctgg cgcgccagct caatactgcg ttccagctca gaaacagaag
     7861 gagtttcttt gggagccttg ccttttgccg ccagctggag caaagcggtc agctctttga
     7921 tctgcttcgt cgtctgagca agcactgttt cccgctcttt caaccgggcg agtttctgcg
     7981 tcgcctgctc tttttcgaga gcggcaaagt gcacaatttc tctaagtgca gcgtcgcgct
     8041 gcgcaacgag agcgcggtat tcgcgcgctg cagccatata gtcggcgtct ttgtaaggcg
     8101 gcgtaccttt ttcgcgcaga acatccagcc gctgactttt tttggcagca agcagcttta
     8161 gctcagccgc aaaacggcta tcttcggcga tgaacttttg cagaaaatcc ggcgcatcct
     8221 tttctgcagc gctcgccagt cgcgcacgct caacgaccgg cagctgcatc ggcacaggcg
     8281 caaaccgcac ctctagagaa gaacgcgcca gccgaatttc gcgcccacgc ttaccgccca
     8341 gctcgcctgt gctgtgaaag tactcatcgg gctcggtaaa aacccgccgg acgtaatcag
     8401 aaaactcgcc gctgcgcaaa agcgcccagc tctcggcggt atcggccact gcacgcattc
     8461 cggtatcgag cagcgggccg ggcatcttca gcttcttgcc gccttttcct gcctcgactt
     8521 ctgcagagag attttccagc tcggtaccgt acgaaagaat acgtatagtc tgccgctcaa
     8581 actgcatgcg gtcaagcttc gcctgcaggt acggatcaaa cacgcggctc aaatcgctca
     8641 tcagcagcgt gcggttcttc gcgatctgcg cgcgcttgcg ctcgagctcc tgttcgtcgc
     8701 gcttgccgct caggttctct tcgagcagcg ctgcagcgat ctctgcttcg gccacgcgcg
     8761 cagcggtctt tttccagcct tcaaacacga ccttgtccgg tgcgcgcggc agtgaggcgg
     8821 cgacagcaga aagcagctgg ttttcaaaat ccttcagatt tttttccgtc aggtaggtac
     8881 tgtaataggt atcgaatctg cgggtctggt aagaatactg tacacgagcg ccgaatgtct
     8941 ttacgaaact gcgccacgtg ttccccagcg atttctcgcg tacctgcact gccttacgga
     9001 gcgcgcgggc agcagcagtc ttttctttca gccggatgcg aaacgttt
//
LOCUS       DAOQJF010000109         8910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4188_length_8910_cov_4.180802, whole genome shotgun sequence.
ACCESSION   DAOQJF010000109 DAOQJF010000000
VERSION     DAOQJF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4188_length_8910_cov_4.180802"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..832)
                     /locus_tag="PLF85_07340"
     CDS             complement(140..832)
                     /locus_tag="PLF85_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19515.1"
                     /translation="MLRYALILLLAAFLVQSVGAIPFYWEPKTEEPGFTIDVPAKWNQ
                     ASRSRDKVANVHFERRDRAGRVAIEVRAYTTDNTDIEQLVLQLRSRLAVKYDRVFLVR
                     RKDVGFRKNMERQVWSARVGKKNYTLLTSFVVADDKVLQLVCVAPAQSKKEYEYVFDN
                     ALLSLDFSDGKPDGGAAAEKTEAPAAAAPAPAPAAPAPAAPVPALPAVPGAPAAPALP
                     DPGKVKPPKIEF"
     gene            917..1258
                     /gene="yidD"
                     /locus_tag="PLF85_07345"
     CDS             917..1258
                     /gene="yidD"
                     /locus_tag="PLF85_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNE19516.1"
                     /translation="MRYWVLLACFLAVVGIGAEEPWSISRQHPVTREAIRPEERPTHL
                     PGNMLIRFYQNYVSPLSGSTCHYSPTCSRYTAEAVARFGLIKGIVMGTDRLIRCHPGQ
                     REYPIDLPTDY"
     gene            1258..2217
                     /locus_tag="PLF85_07350"
     CDS             1258..2217
                     /locus_tag="PLF85_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19517.1"
                     /translation="MRNLLTRLLALLLLPLIYAPAAQAQPKRSAPDFTRPQTFLRLAD
                     AFADEQDYYRAITEYKKVIHLFPDYEKLEWVHFQIGKMYYEGGRYAQAKHELLPLTDS
                     RDKYLSFLSHNYIALSYYENTEYANAERLFGELRQHEAGKTYTTDYTIYMSMAAAGNK
                     KFSDSLERMREAEKSWKDRQAPGTNTGGAEPQYQQFFEKTLPLLEKAAGLSNKSPFWA
                     ATFSALFPGGGHFLLGEWDTGIVSLSLVGGAAFLAYDGFMRDSPVQSILFTTFATGAY
                     IGQIYSSYRTARKYNEELGDTEFRELNRQFRSLNVALQFQTRF"
     gene            complement(2219..2689)
                     /locus_tag="PLF85_07355"
     CDS             complement(2219..2689)
                     /locus_tag="PLF85_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19518.1"
                     /translation="MRKVLPNPDKESRRGLFRCRRGGVFLLLLPLALVAACAVGEPLP
                     QDYYSGTDKIAGGQAPSVPVVTYTPATRRFDFTASIDPDTGSEVGNYIVYYYNGVPTK
                     YYEQRYIDGIIPVGSARTFFFTGSPGTYTVVVTGFDGFRESAVTDANRITFTLP"
     gene            complement(2664..3218)
                     /locus_tag="PLF85_07360"
     CDS             complement(2664..3218)
                     /locus_tag="PLF85_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19519.1"
                     /translation="MRLLLCSCLLALAVGITACTAGVAHTVTVVLPTPHLNPAGKARQ
                     FGTLYFYTDRNMPETSAAFSALLSGQTAQALDALGKIEGANLPAIQRAYWQNDVAVCF
                     ILEGRYKEADELLLQAGLTADEDAIRHNHRVSVYLNDSYNLHKTRQRPVVVPEGTPAE
                     ATSAPSAESKPDVKKTDEKGAPKP"
     gene            3300..4244
                     /gene="trxB"
                     /locus_tag="PLF85_07365"
     CDS             3300..4244
                     /gene="trxB"
                     /locus_tag="PLF85_07365"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNE19520.1"
                     /translation="MSQNHFDTIIIGSGPAGYTAAIYAGRANLKTLVLEGIERGGQLM
                     ITSEVENYPGFPQGIQGPEIMEHFRSQAARFGAELKSVMADKAELTGGAPFKITAEGT
                     LYTSDSVIIATGASARFLGIPSELAYKGKGVSACATCDGFFFRGKEVAVVGGGDSAFE
                     EANFLTKFASKVTLLHRRDTFRASKIMVDRARNNPKIVIKPFAEVAEIYGEEKTGTVS
                     GLKLKNTETGALEDFPVQGLFIAIGHVPNTSLFKGQLNMDETGYLVTQPDRTATNIPG
                     VFAAGDVKDHFYRQAITAAGSGCQAAIEAERYLESKGH"
     gene            4246..5169
                     /locus_tag="PLF85_07370"
     CDS             4246..5169
                     /locus_tag="PLF85_07370"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNE19521.1"
                     /translation="MEDFESKPAWFDGKFVALKDANLNVRTHALQYGTAFFGGMRAYY
                     NSNKKNLYLVRLEDHHARLRASARILQMHWNMPYAEFRDITLELLRKGEWRENCYLRP
                     FIYKSELSLSPRLHNVEDKFALYAIPLNDYLDTKRGVRAAVSSWTRLSDNQIPARAKA
                     TGGYINSALAKSEALENGFDEAIFLDSRGFVSEGSAENIFVVRDGKLITPGPSSAILE
                     GITRRTVLKLAQDMGIETLERDISRTELYVADEVFFTGTGAQVAWVESIDRRTIGAGK
                     IGPLTEKLQKTFFSYATGEIPEYMNWLTPVY"
     gene            5174..6124
                     /locus_tag="PLF85_07375"
     CDS             5174..6124
                     /locus_tag="PLF85_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19522.1"
                     /translation="MLHITAANCPQTVIFEGDMSVEKAAHLRQLGTEILYEHRRDTAD
                     SRALVSTLVHEGQHPDLVEFGGETLLIGPEKDPPPGTVRHLLRRILPYAPWKSHARVV
                     IFQNAGGIKDEAETALLKTLEEPATQHYFFLSVQSTEELKETIRSRSIITRVVNRHVV
                     PEGMTDPWQRFYYLMGVKDFMAQWPEATERITGDTRQAFDELAFTADDFTALERVLFL
                     LPKQLFDKESVTTQGKALRFAVLPLLAALRDRATQGVVAPFSPLVLNRMTPGQAIEAS
                     TLVHNYLRHLEYRVFGNRPLNLHAVFYSFFFRFMPLWSPS"
     gene            6215..7273
                     /gene="nuoH"
                     /locus_tag="PLF85_07380"
     CDS             6215..7273
                     /gene="nuoH"
                     /locus_tag="PLF85_07380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNE19523.1"
                     /translation="MWYHSEIFWVIVKIVILFGAMITGAAYYTLLERKWAGYIQDRYG
                     PNRAGLWGLLQPMADGIKFLTKQETIPRNADAWIFVLAPALSMTTAVMLWAVIPFSPV
                     ITQLPDAVKEITGKSDFAFQVMNPSGGLLYMSAVASLAVYGIMLAGWSSNNKYALLGS
                     VRATAQMISYELALALSLAAIVILAGSLDLRQIVHAQEERWFIATLPGALAFFIFLVT
                     MFAETNRLPFDLAEAESELVVGFHTEYGAFKFALFFIAEYMNMITLSLLAALLFFGGY
                     NLPGFLPASVKAAWYAPIVGSLFYFAKAFFFVFLFVWVRWSLPRFRYDQLMNLGWRRL
                     VPVAMANLLIAGIWVYVF"
     gene            7287..7493
                     /locus_tag="PLF85_07385"
     CDS             7287..7493
                     /locus_tag="PLF85_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19524.1"
                     /translation="MSGDQEEQPLDVRQMALLFLNSVVFIKIPLLFFGFRMSEDPTPF
                     NIGGFVLCVVISVVSLTRFLLKKK"
     gene            7557..>8910
                     /locus_tag="PLF85_07390"
     CDS             7557..>8910
                     /locus_tag="PLF85_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19525.1"
                     /translation="MNSAISHLQLLVLLIFTGCTGQLQQFVDEDKPLVLSVVSNTAAT
                     PTYSVRNGTISFSITLQVDRTANYSILHGSGCSATRQPAGVAVTGSLQKNVSMTISVA
                     ILIADVSNYGSKVVVCAADTANYKSASTTLDFSSAMTYLNSLNELTGNGLVADATSAF
                     QIFQADAVSGSPNRGTTANNNTASRPYQHAVFIDPNDGNKMKYFVADRDNHRVLIFNS
                     LPTGNTATADVVVGQTNFINATINAGGGAVVSAQGFNEPCAVAVSNTGKMLIVDRVNN
                     RVLIYNTIPTANGVAADFVIGQLYLTTGTANNFFDLTTGDKVLSDPVSVHIIAGKVYI
                     TDRNNNRVLVFNSIPTANGVAASFVIGQPNFSTVTTGTDYTNNSFLNHPYELTVSGTR
                     LYLADADNHRVLVFDPLPTAATDKPIHVIGQTSPNLGSANQGGANPTDQTLNFPVSVA
                     I"
BASE COUNT         1940 a         2589 c         2427 g         1954 t
ORIGIN      
        1 gctttcaggt cgaccgcttc atcagaagct tctgcttttg cttctggttc cgggtctgcc
       61 cccatgagca tgtaagatcc cgaaagccca agcaggcaga gtagcgaaag gtattggatt
      121 acggttttca tgtttcctct tagaattcga tttttggcgg cttgacctta ccggggtcgg
      181 gcagtgccgg ggcggctggc gctccgggaa cggctggtaa tgccgggacg ggagctgccg
      241 gtgccggggc ggctggtgcg ggcgctggcg cagctgcagc aggcgcttcg gtcttctctg
      301 ccgctgcccc accatcgggt ttaccgtcag aaaaatccag cgagaggaga gcgttgtcaa
      361 agacgtactc gtattccttt tttgactgtg ccggggcgac gcagaccagc tgcagcacct
      421 tgtcgtcagc gacgacgaat gacgtcagca gggtatagtt ctttttgcct acgcgggcgc
      481 tccagacctg ccgctccata ttcttgcgga agccgacgtc tttgcggcgg accaggaaca
      541 ccctgtcata tttgactgcc agccgcgagc gcagctgcag cacgagctgt tcgatgtcag
      601 tattgtccgt agtgtaagcc ctgacctcga tcgccacccg gccggcccgg tcgcggcgct
      661 cgaagtggac atttgccacc ttgtcgcgtg agcgagaagc ctggttccat ttggccggta
      721 catctatggt gaaacccggc tcttcggtct tcggttccca gtagaacggt atagctccca
      781 cggattgtac caggaaggca gcgagtagca ggattagggc atagcgtagc atatagtctc
      841 tcctttagat aatcggcatg cgtacaacat aaaattaaac cattgacacc gtccacgcga
      901 ctaaatttcg caggtcatgc ggtattgggt tttgttagcc tgttttctgg ctgtggtggg
      961 cattggcgct gaagaaccct ggagtatttc acgccagcac ccggtcaccc gcgaggccat
     1021 acgccccgaa gaaaggccca cccacctgcc cggcaacatg ctgatacgct tctaccagaa
     1081 ctacgtcagc ccgctcagcg ggtcgacctg ccattattcg ccgacctgct cgcggtacac
     1141 ggccgaggct gtggcccgct ttgggctgat caagggcatt gtcatgggta cagaccggct
     1201 gatccgttgc cacccgggcc agcgcgaata cccgattgac ctgcccacgg actactgatg
     1261 cgcaacctgc tgacccgcct tttggccctc ttgctgctcc cgcttattta tgcacctgcc
     1321 gcacaggcac agcccaaacg cagcgcgccg gattttaccc ggccacagac atttttgcgg
     1381 ctggccgacg cctttgcaga cgagcaggat tactaccgcg cgattaccga gtataagaag
     1441 gtaatccacc tgtttcccga ttacgaaaag ctcgagtggg tacatttcca gatcggcaag
     1501 atgtactacg aagggggacg ttatgcgcag gcgaagcacg agctgctgcc gctgaccgat
     1561 tcgcgcgaca agtacctcag ctttctctcg cataactata tcgccctctc ttactatgag
     1621 aacaccgagt acgccaatgc agagcgcctg tttggcgagc tcaggcaaca cgaggccggc
     1681 aaaacctaca ccacggacta taccatctac atgagcatgg cggcagcggg aaataaaaag
     1741 ttcagcgact cgctcgagcg catgcgcgag gcagaaaagt cctggaaaga ccggcaggca
     1801 ccgggtacaa ataccggcgg cgcagaaccg cagtaccagc agtttttcga gaagaccctg
     1861 ccgctgctgg agaaagccgc gggtctctcg aacaagagcc cgttctgggc ggcgaccttc
     1921 tcagcgctgt ttccgggtgg cgggcacttt ctgctcggcg agtgggatac cggcatcgtg
     1981 agcctgagcc ttgtgggtgg tgcggcattt cttgcctacg atggttttat gcgcgacagc
     2041 ccggtgcagt cgatactgtt cactactttc gccaccggcg catacatcgg gcaaatctat
     2101 tcgagttacc gcaccgcgcg caagtacaac gaagaactgg gcgatacgga gtttcgtgaa
     2161 ctcaaccggc agttccgcag cctgaacgtc gcgctgcagt ttcagacacg gttctgaact
     2221 acggcagcgt aaacgtgatg cggttggcgt cggtcaccgc cgattcgcga aatccgtcga
     2281 agccggtgac gacaaccgta tacgtgcccg ggctaccggt gaaaaagaag gtacgcgcac
     2341 tgcccaccgg aatgatgccg tcgatgtagc gctgctcgta gtatttggtc ggtacaccgt
     2401 tgtagtagta cacgatatag ttgccgacct cagaaccggt atcagggtcg atcgaagcgg
     2461 taaagtcgaa gcgccgcgtc gcgggcgtat aggtgacaac cggtaccgac ggcgcctgcc
     2521 cacccgcgat cttgtctgta ccactgtaat agtcctgcgg cagcggttca cccacggcgc
     2581 aggcagccac cagggccaaa ggcaacagca gcaggaacac tccccctcgc ctgcagcgga
     2641 ataacccgcg ccgtgattct ttgtcagggt ttgggagcac ctttctcatc ggtttttttc
     2701 acgtcaggtt tgctctcggc agacggtgcg cttgtggctt cagccggtgt accttcgggc
     2761 accacgacag ggcgctggcg cgtcttgtgc aggttgtacg agtcgttcag gtagacgctc
     2821 acccggtggt tatggcgaat ggcatcttcg tcggcagtga gccccgcctg gagcagcagc
     2881 tcgtcggcct ctttgtagcg cccctcgaga ataaagcaca ccgcaacatc gttttgccag
     2941 taagcccgct gtatggccgg caggttggca ccttcgatct tgcccagcgc atcgagcgcc
     3001 tgcgcagttt gaccggagag caaggcgctg aaggcggcgc ttgtttcggg catattacgg
     3061 tcggtgtaga agtacagcgt gccaaactgc cgggctttgc cggccgggtt aagatgaggc
     3121 gtcggcagca caaccgtgac agtgtgggca acaccggcag tacaggcagt aatacctaca
     3181 gccaaagcca gcaggcagga gcataacagg agccgcataa gtccttttgc aggtttccgg
     3241 agctgcagca aagacatttt attgtccgca tgtctgcctt tccattattt tcgtggcata
     3301 tgagccaaaa ccatttcgac accattatca tcggttccgg acccgcgggt tatacagcgg
     3361 ctatctacgc cgggcgcgca aacctcaaga cccttgtact cgagggtatc gaacgcggcg
     3421 gccagctgat gatcacaagc gaggtcgaaa actatcccgg ctttccgcag ggaatccagg
     3481 gcccggaaat catggagcac tttcgctcac aggcagcgcg ctttggcgca gagctcaagt
     3541 cggtgatggc agataaggcc gaactgaccg gaggagctcc ttttaagatc acggcagaag
     3601 gcacccttta cacttctgac tcggtgatca tcgcaacagg cgcttcggcg cgttttctcg
     3661 gtattccttc tgaacttgcc tacaaaggca aaggtgtttc tgcctgcgct acctgcgacg
     3721 gttttttctt ccgcggcaaa gaagttgcag tagtcggcgg gggagattca gcttttgaag
     3781 aagcaaactt cctgaccaag tttgcatcaa aagtgacgct gctgcaccgc cgcgatacct
     3841 tccgcgccag caagatcatg gtcgatcgcg cacgcaacaa ccccaagatc gtgatcaaac
     3901 cctttgccga agtcgctgag atctacggtg aagaaaagac cggcacggtt tcgggtctca
     3961 aactgaaaaa taccgaaacc ggtgcgctcg aagacttccc ggtacaggga cttttcatcg
     4021 cgatcggtca cgtacccaac acgtcgctct tcaagggcca gctcaatatg gatgaaaccg
     4081 gctacctcgt cacccaaccc gaccggaccg cgaccaatat tcccggcgtg tttgccgctg
     4141 gcgacgtgaa agaccatttc taccgccagg cgattacggc cgccgggtca ggctgccagg
     4201 cagcgataga agccgaacgt tacctcgaat cgaaaggaca ctgaaatgga agattttgaa
     4261 agcaaacccg cctggttcga cggtaaattt gtcgcgctga aagacgctaa cctcaatgta
     4321 cgcacgcacg cgctgcagta tggcacggcg ttctttggcg gcatgcgcgc ctactacaat
     4381 agcaacaaga agaaccttta ccttgtcagg ctcgaagacc accatgcacg gttgcgtgcg
     4441 tctgcgcgta ttctgcaaat gcactggaac atgccttatg ccgagtttcg tgacatcact
     4501 ctcgagctgc tgcgcaaagg cgaatggcgc gaaaactgct acctgcgtcc ctttatctac
     4561 aagtcggagc tttccctctc tccccgcctg cacaacgtcg aagacaagtt tgcgctctat
     4621 gcaataccgc tgaacgacta cctcgatacc aagcgcggtg tacgcgcggc ggtttcatcg
     4681 tggacacgct tgtcagacaa ccagataccc gcgcgcgcaa aggccaccgg cggttacatc
     4741 aactccgcgc tggcaaagtc cgaagcgctg gaaaacggtt tcgacgaggc aatctttctc
     4801 gatagccgcg gttttgtttc cgaaggctca gcagaaaaca tctttgtcgt gcgcgacgga
     4861 aagctcatca cgccaggccc gtcgtcggcg attctagaag gcatcacgcg ccgcaccgtg
     4921 ctcaaacttg cacaggacat gggaattgaa acgctcgagc gcgatatcag ccgcaccgaa
     4981 ctctacgtcg ccgacgaagt attctttacc ggaaccggcg cgcaggttgc ctgggtagaa
     5041 agcatcgacc gccgcaccat tggcgccggc aagatcggtc ctcttaccga gaaactgcag
     5101 aagacattct tctcgtacgc gacgggtgaa atacccgagt acatgaactg gctcaccccc
     5161 gtctactgag cgcatgctgc atatcaccgc cgccaactgc ccgcagacgg tgatttttga
     5221 gggcgacatg tcggtcgaga aggcagcgca cctcaggcaa ctgggcacag aaatcctgta
     5281 cgagcaccgc cgcgacacgg cagacagccg ggcactggtt tcgacgcttg tccacgaagg
     5341 ccagcatccc gacctggtag agttcggcgg cgagacgctg ctgatcggcc cggagaaaga
     5401 ccctccaccc ggtaccgtgc gccacctgct gcgcaggatt ttgccctatg caccgtggaa
     5461 atcccacgcg cgcgtggtta tcttccagaa tgcaggcggc atcaaagacg aagcagaaac
     5521 ggcgctgctg aaaacactag aagaaccggc gacgcagcac tactttttcc tgagcgtaca
     5581 atcgaccgaa gaactcaaag agactatccg ctcgcgctcc atcatcacgc gcgtggtcaa
     5641 ccgccacgtg gtgcccgagg gcatgactga cccatggcag cgtttctatt acctgatggg
     5701 agttaaagat tttatggcgc agtggcccga ggcgacagag cgcatcacgg gcgatacgcg
     5761 gcaggcattt gatgagctgg catttaccgc cgatgatttt actgcgctcg agcgtgtgct
     5821 ttttcttctg ccgaagcaac tcttcgataa agagagtgtg acgacgcagg gcaaagcatt
     5881 gcgttttgct gtcctgccct tacttgcagc cctgcgcgac cgggcaactc agggtgtggt
     5941 cgccccgttc tcaccgctgg tgctgaaccg catgaccccg ggccaggcaa ttgaagccag
     6001 cacactggtg cacaactacc tgcggcacct cgaatacagg gtatttggca accgtcccct
     6061 gaacctgcat gcagtttttt acagtttctt tttccgcttc atgccgcttt ggtcgccgtc
     6121 ctgacagggc gccgctcaag gccggtgaca ctgtaaaccg ccaaacccat tctgctcgac
     6181 acgctcagtc aagggggcag cctgactgta ttctatgtgg taccatagcg aaattttctg
     6241 ggtaatcgtg aagattgtga tacttttcgg tgcaatgatc accggagcgg cctactacac
     6301 cctgctggag cgcaagtggg ccggttatat ccaggatcgt tacgggccaa accgcgccgg
     6361 cctctggggt ttgctgcagc cgatggctga cggcatcaaa tttcttacca aacaagagac
     6421 aataccccgc aacgcggatg cctggatttt tgtactcgca cccgcgctgt cgatgacaac
     6481 tgccgtgatg ctctgggcgg tcattccctt ctcgccggtg atcacccagc tgccggacgc
     6541 agtgaaagag atcacgggca agagtgactt cgccttccag gtcatgaacc cctcgggcgg
     6601 cctgctctac atgtctgccg tagcttcgct cgccgtttat ggcatcatgc tcgcaggctg
     6661 gtcgagcaac aacaagtacg cgctgctggg ttcggtacgg gcaacggcgc agatgatctc
     6721 ttacgagctt gctctcgctc ttagcctcgc ggcgatcgta atcctggcgg gtagtctcga
     6781 cctgcggcag attgttcacg cgcaggagga gcgctggttt attgcgacct tacccggggc
     6841 tctggctttt tttatctttc ttgtcacgat gttcgccgag acaaaccggc taccgtttga
     6901 cctcgccgaa gcggaaagtg aactcgtggt gggtttccac accgaatacg gcgcgtttaa
     6961 atttgcgctc ttcttcatcg ccgagtacat gaacatgatc acgctttcgc tgctcgcagc
     7021 gttgctgttc tttggcggct acaacctgcc aggctttctg ccagccagcg tgaaggccgc
     7081 atggtatgcc ccgattgtgg gttcgctgtt ctactttgcc aaggccttct tcttcgtctt
     7141 cctgttcgtc tgggtacgct ggtcgctgcc gcgcttccgc tacgaccagc tgatgaacct
     7201 cggctggcgc agactggtac cggtggccat ggcaaacctg ctgattgcag gtatctgggt
     7261 ctacgttttc tgacagacag cctgccatga gcggcgacca agaggagcaa ccgctcgatg
     7321 tcaggcagat ggccctgctg tttctcaact cggtagtctt tatcaaaata ccgctgctct
     7381 tcttcggctt cagaatgagc gaagatccaa cccccttcaa tatcgggggt tttgttttgt
     7441 gtgttgtgat ctcagtcgtg agcctgacac gttttttact gaagaagaaa taatggttgc
     7501 cggtgccccg tttcctgcgt cctatagtat gttcggcggc caattcagtg cgtaaaatga
     7561 actcagccat ttcccatctt caactcctgg ttctgctaat ttttacgggc tgcaccggtc
     7621 agctgcagca gtttgtcgat gaggataagc cgcttgtgct tagcgttgtc tctaacacgg
     7681 cggcgacgcc gacgtatagc gtgagaaacg ggacaatttc tttcagcatc acgctgcaag
     7741 ttgacaggac tgccaattat tcgattttac atggctctgg ctgcagcgcg acccgccaac
     7801 ctgcgggtgt cgcggtgaca gggtcgctgc agaaaaatgt ttcgatgaca atctcagttg
     7861 cgatcctgat cgccgacgtc tctaactacg gttcaaaggt tgtcgtctgc gctgccgata
     7921 cggcaaatta taaatccgcc tcgactacac tggattttag cagtgcgatg acgtatttga
     7981 atagcctcaa tgaactcacc ggcaacggcc tggttgccga tgcgacctcg gcttttcaga
     8041 tatttcaggc cgacgccgtg agcggtagcc caaatcgcgg caccactgct aacaacaata
     8101 ccgcgagcag accttaccag cacgccgtat tcatcgaccc gaacgacgga aacaaaatga
     8161 aatactttgt tgccgaccgg gacaatcaca gggttctcat ctttaacagc ttgcccaccg
     8221 gtaacacagc aacggccgat gtggttgtgg gacaaaccaa ttttatcaat gccacgataa
     8281 acgccggggg gggcgccgtt gtcagcgccc agggattcaa tgagccttgt gctgttgccg
     8341 tcagcaatac aggcaagatg ctcattgtcg acagagtcaa caacagagtg ctgatttaca
     8401 acacgattcc aacagcaaat ggcgttgccg cagattttgt tatcgggcaa ctctacctga
     8461 caaccggaac cgcaaataat ttttttgatt taaccacggg agataaagtg ctcagtgatc
     8521 cggtctcagt acatatcatt gcaggcaagg tatacatcac tgatcgtaat aacaaccgcg
     8581 ttcttgtctt caattcaatt ccaactgcaa acggagtcgc agctagcttc gtcataggcc
     8641 aaccgaattt ctcgactgtt actaccggca ccgactacac caataatagc ttcctgaatc
     8701 atccatacga gctaactgta tctggaaccc gcctctatct cgccgacgcc gataatcatc
     8761 gggtcttagt ctttgacccg ctaccgaccg cggcgaccga taaaccgatt catgtgatcg
     8821 gccaaacttc gccaaacctt ggttcggcca accagggcgg ggcaaatccg actgaccaaa
     8881 ccctcaattt tcccgtcagc gtagcaatca
//
LOCUS       DAOQJF010000110         8904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4197_length_8904_cov_5.720872, whole genome shotgun sequence.
ACCESSION   DAOQJF010000110 DAOQJF010000000
VERSION     DAOQJF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_8904_cov_5.720872"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="PLF85_07395"
     CDS             <1..1543
                     /locus_tag="PLF85_07395"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNE19526.1"
                     /translation="LMAIAFNELLTMMPQLILVVGGLLVLSSQMLLKQEGRTRTTGAI
                     ALGTVLVALVVVVFGLSDATGSKTVLPRAFLGTDAVSAINNSFRYSAFSANALVVILS
                     LAAGVLLLMRVVLPKYNIDFAENHFLLLMSVVGYAYAFCAEDLVTLFVALELGTLPII
                     VLVGINRESRAANEAAVKYLLLSAFAMAFFLLGIALLYAANSTVRLREIREIPPHFSK
                     TRVMVLAYLFLFAGFLFKLGAVPLHSYIADVYEGCTTVFTGLLASLSKVASVLVFFKV
                     AMSVHDGYRQYLAPIATVAAVASMLYGSFAAIGTRNLKRILAYSSIGHAGYMLCFFVV
                     PASVDPGAMGTIKQDAGSSLYIYVVGYAAASLLAFGAIALMELRSGTGEVLTLDNLQP
                     GKTSRVVSIALGLSLLSFMGMPPLAGFFGKFFLFKYLALSGNITLAAVAGLSSAIAMY
                     AYVRVLRPLFFSDAEAGIAVTQPEAGNRGVQLALGLLVAVLAFFAVAGGFLYNTGVQA
                     VHRIY"
     gene            1571..2740
                     /locus_tag="PLF85_07400"
     CDS             1571..2740
                     /locus_tag="PLF85_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE19527.1"
                     /translation="MNQRKHRFSTLGIANLTMFVFLIGVAFSPFAGTCGGGSSIVEAT
                     TNNPLLKSDSIQYAEKLQSAVREIYKEVNPAVIRIETEQTVEFNHPFFNDPMFRRFFQ
                     VPEGQQKQKRAGLGSGFVISADGYAVTNHHVVQKVDKITVKLTNGKEYQAKLIGSDPN
                     SDIALIKIEGAKGLKTAYLGDSDKIEVGDFALAIGNPFGLQSTLTTGIISSKGQDVNS
                     ADGVSRIQTDASINPGNSGGPLLNIRGEVIGINQMIYSQSGGSVGIGFAIPINHAKHV
                     IEKLKKGQKIRQGYIGVSVDPEVTEEKLKLLGVEGKTGLIVAQVVIGSPAFKAGIAAN
                     DFITHIDGKPAEKFSVLKAAVLQKGVGAELEIKLIRQGKEQTVSVKVAEAPQQPR"
     gene            2831..3595
                     /locus_tag="PLF85_07405"
     CDS             2831..3595
                     /locus_tag="PLF85_07405"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNE19528.1"
                     /translation="MTLTCDVGNSHSVVGLWKDGQLLETLRLATDGRRTADEWRIFLT
                     AWLRSIGEGSIRKAAFCSVVPAANEAIGEALRSLGVDAPHSLNSSSRLNFGFDYPAPQ
                     TLGADRIADMIAAVYYFGKNCVVLDFGTAITFSVVSDGKFRGGVIAPGITSSLEALFS
                     STAKLPKIAFRRTSKAIGSSTTESIEVGAYVGWRGVVREILSEIRRELPQNGAGYKVV
                     ATGGISESLDFAPEFFDVVDKNLTLKGLYLSVSDAY"
     gene            3620..4855
                     /locus_tag="PLF85_07410"
     CDS             3620..4855
                     /locus_tag="PLF85_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNE19529.1"
                     /translation="MTKLRYLTFLKVRTLPLLDGYLLKHFLPSLLVASTFFTFIVLVF
                     FLKETIKHAVEKNLEFGVILELCWYALGWTLTLTVPMAFLLSTILTVGALNADSEIIA
                     LRAGGITYPRIMRPFVGVAVVFFFILFWFSNFVVPRFADNMENMRQYILTTDPIAAIQ
                     PGQFITLDKREGNVRKIYIERAVKDASFRGEILQNIQVRKIGNVGGVRRLTEIIIAQE
                     GRKVLKQTPNGEWVKALRLTRGYLFTYGLDGTFQRVDFSGGMFDLNIQEPERIKKMKR
                     IEDMGSLTLPQLIGSFETIKSYPKADELARRAHLEIHKRIALSLSVVIFVFIGFPLAI
                     VNRRSGKGMGLGISIIFIFAYFALFLSAETFSVRQQWVSPAIAAHTANFLMALGAGWF
                     SLTRLAEKSLSSIIFRKRV"
     gene            complement(4898..5959)
                     /locus_tag="PLF85_07415"
     CDS             complement(4898..5959)
                     /locus_tag="PLF85_07415"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE19530.1"
                     /translation="MTEAPLAEARFFLVGEEPGVASALVAYLRERGAETQVIARLEEL
                     DLNEETENVLVAHYSGTAGLAHRLPILKRHDSALKVVLLHEITERGQIDTALLSVTDH
                     LLEKPFTRQALEKALAGLKFHPLSGKSVYLFESGDRLAGRVLRTLGASVVTEAGTIDT
                     KAPPELAVMAPEAIDDTFRVALAVFRQFCPDVPIFMLYDPQAAGTLDSDILKEVAYLV
                     QKPVTRPVLRQKLLAYFEQPLKDRRKNPRKKGISQMWISAFNTELGTPELFESPYLID
                     ISQSGLSFQSHIRYNEGQLMAIWIVAEDYPDKIIDLRGNIRWKKHDGEPASFFKYGLE
                     FTRQDSDAYRTFARMIAMH"
     gene            complement(5996..6370)
                     /locus_tag="PLF85_07420"
     CDS             complement(5996..6370)
                     /locus_tag="PLF85_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE19531.1"
                     /translation="MSDKKRILIVDDSGYMRQVIRRHLESKGFEVVGEATDGSEVVAM
                     AESLKPDLITMDLSMKNLGGIEATKLVKKAVPDAKVIVVSALGESRFIKEAVTAGAHD
                     FIIKPFTEERLISSVKSALKIS"
     gene            complement(6401..6619)
                     /locus_tag="PLF85_07425"
     CDS             complement(6401..6619)
                     /locus_tag="PLF85_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19532.1"
                     /translation="MWQKIKTYTIAHLLGLALLLGGWYLSIMNTSADRWQSVKFLSGA
                     NLVGLIMILVGAYLPEIWISIKNRGAKS"
     gene            7434..>8904
                     /locus_tag="PLF85_07430"
     CDS             7434..>8904
                     /locus_tag="PLF85_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19533.1"
                     /translation="MKSAYRWKLLLGLGLIAFLAACAPKRKETQFSKNKEAEAGQTYT
                     EDQLRAALGSDRYDALVTGIGAANLNLLTYGIGISNMTQMINGISAPGKLTSLMSDGA
                     NSRKLNAIEVLDLLNKLDDACQRPGSFGTDTIGKMVNMINNVTLAGMEGIKNIVHGVQ
                     DQPADLNGTAYQNGVARLGFLVSLLNENFSVMPTLVNDLAAPGSVFSASGNQKLIRLV
                     NSSLDVRDLAVIINGTTNLSNITAVMAGLTGNMFCSKPEYQTPATCSSGGGTWNNTLC
                     SVGSPNSPHVTRTACAGAGGTWTPDGIENMTRLINELDRVCSNTSYTNGKACIDAGAT
                     WRTKASKIPVIVNNITNVPNMFTIVNGLSNDGKRPQWPVGGVTVALPVGLGLPTAYAG
                     DAGNVFDGVDSMVATINKLYVTGAGDADSFGTEGMKRMAYLVNNLDTTGVFPNDTNFD
                     DITGGFNCAVGNFDNRLNWAFSNNGSAAAPFNGRSWASDN"
BASE COUNT         1907 a         2388 c         2402 g         2207 t
ORIGIN      
        1 gctaatggct attgctttca atgaactgct gacgatgatg ccgcagctga tcctggtggt
       61 cggcggttta ctggtgctca gttcgcagat gctgctgaag caggaaggcc gtactcgtac
      121 aaccggtgcg attgctctgg ggactgtact ggtcgcgctg gttgttgtcg tttttgggct
      181 cagtgatgcg acgggcagca agactgtcct gccgcgtgcc tttctcggta cggatgccgt
      241 atcggcgatt aacaacagct tccggtattc agcgttcagc gccaatgcgc tcgttgtcat
      301 tctttcgctc gcggcggggg tgctgctgtt gatgcgcgtg gtactgccga aatacaacat
      361 tgattttgca gagaaccatt tcctgctgct gatgagcgtt gtcggttacg cgtatgcctt
      421 ctgcgctgaa gatttggtca ccctcttcgt ggcgctggaa cttggtacct tgcccatcat
      481 tgtgcttgtg ggtataaacc gcgagtcgcg cgcagcaaat gaagctgccg tcaagtatct
      541 cttgctctcg gcttttgcca tggcattctt cctgctcggt atcgcgctgc tctatgctgc
      601 gaactcgact gtacggctgc gcgaaatacg cgaaattcct ccgcattttt cgaagacgcg
      661 tgttatggtg ctggcgtatc tgttcctgtt tgccggtttc ctgttcaaac ttggtgctgt
      721 accactgcac agttatatcg ccgatgttta tgaaggctgc accactgtct ttacgggcct
      781 gctggcttcg ctctcgaagg tggcttctgt cctggttttt ttcaaggttg ccatgtcggt
      841 gcacgacggt taccggcagt accttgcgcc gatcgctacg gtcgcggctg tggcatctat
      901 gctgtacggg tctttcgcgg caattggcac gcgcaacctg aagcgaatac tggcgtattc
      961 atccatcggc cacgcgggtt acatgctctg cttttttgtt gtgcctgcct cggtcgaccc
     1021 cggcgctatg ggaaccatca agcaggatgc ggggtcttcg ctttatatct atgtggttgg
     1081 ttatgcggcg gcaagtttgc tggcattcgg agcgattgcc ctgatggaat tgcgctccgg
     1141 tacgggcgag gtcctgacgc tcgataacct gcaacctgga aaaaccagcc gggttgtctc
     1201 tattgcgctt ggcctgtcgc tgctgtcgtt catgggtatg ccaccgctgg ctggcttttt
     1261 cggaaaattt ttcctcttta aatatcttgc tctttcgggt aatattactc tcgctgccgt
     1321 tgcgggtctc tcgagcgcga ttgccatgta tgcctacgtg cgtgttctgc gaccgctgtt
     1381 cttctctgac gccgaagctg gtatagcagt cacgcagccc gaggccggta atcggggggt
     1441 acagctggcg ctgggcctgc tggttgcagt gctggctttt tttgcggttg cgggcggctt
     1501 tttatacaat acgggagtgc aggcagtcca cagaatctac tagttcaaga ggttaacgat
     1561 gttcagaaaa atgaatcaac gcaaacaccg cttctcgact ctgggaatcg ccaacctgac
     1621 gatgtttgtt ttcctgatcg gggtggcgtt ctcgccgttt gccggtacct gcggcggcgg
     1681 cagttcaatc gttgaagcga cgaccaataa tccgctgcta aaatctgatt cgatacagta
     1741 tgcagaaaag ctgcagagcg cggtgcgtga aatctacaaa gaggtaaatc ccgctgttat
     1801 tcgtatcgag accgaacaga cggtagagtt caaccatccg ttttttaatg acccaatgtt
     1861 ccgtcgcttc ttccaggtgc ccgaagggca gcagaagcaa aagcgcgcgg gccttggttc
     1921 tggtttcgtg atttctgcag acggttatgc agtgacaaac caccacgtgg tgcaaaaggt
     1981 tgataagatc actgtaaagc tgacgaatgg caaagaatac caggcgaagc tgatcggctc
     2041 tgaccccaat tcagacatcg cgctgatcaa gatcgagggc gctaagggtc ttaagaccgc
     2101 ttacctcggc gattcagaca agatcgaggt gggggatttc gcgctcgcga ttggtaaccc
     2161 gtttggtctg caatcgacac tgactacagg tatcatctct tcaaaaggcc aggacgtgaa
     2221 ttccgccgac ggcgtgagcc gtattcagac cgacgcctcc atcaacccgg gaaattcggg
     2281 cggacctttg ctcaacatcc gcggtgaagt tatcggtatc aaccagatga tctattcgca
     2341 atcgggcggc agcgtcggta tcggttttgc gattccgatc aaccacgcca agcatgtcat
     2401 tgagaaactc aagaaaggcc aaaagattcg ccagggttat atcggcgtgt cggttgatcc
     2461 tgaagttacc gaagagaagc tcaagctgct tggcgttgaa ggcaagacgg gtctgatcgt
     2521 ggctcaggtc gtgatcggtt cgcccgcgtt caaagcaggt atcgcggcta atgactttat
     2581 cacccacatc gatggcaaac cggctgaaaa gttctcggta ctgaaagctg cagtattgca
     2641 aaagggtgtg ggagctgaac ttgaaatcaa actgatccgt cagggcaaag agcagaccgt
     2701 ctcggtcaaa gttgccgaag ctccgcagca gccgcgctaa gcgcattccc gtgacgctca
     2761 ccgccaaaca cgatgttgat ggtgagcgtc gggcaggcga tgcggtgagc ctgccgaacc
     2821 gaggccctct ttgacgctca cgtgcgatgt cggtaactcc cacagcgttg tggggctatg
     2881 gaaagacggg cagctgctcg aaaccttaag gctcgccacc gatggccggc gcactgctga
     2941 tgaatggcgc atcttcctca cggcatggct gcgcagcatt ggtgaaggca gcatacgcaa
     3001 ggctgccttc tgttcggtcg tgcctgcggc aaatgaagcg attggcgagg cgctgcggtc
     3061 actcggcgtc gatgcgccgc acagccttaa ctcctcttcg cgcctcaact tcggttttga
     3121 ttacccggca ccacagacac tgggggcaga ccgtattgcc gacatgattg cggcggttta
     3181 ctacttcggc aagaactgcg tcgtgcttga ttttggtacc gcaatcacct tctcggttgt
     3241 cagcgatggc aagttccgtg gcggcgtgat tgctccggga attacgtcgt cgctcgaggc
     3301 gctgttcagc tcaactgcca agctgcccaa gattgccttc cgccgcacga gcaaggctat
     3361 cggatcttcg acaaccgaat cgatcgaggt cggtgcctat gttggctggc gcggcgtcgt
     3421 acgcgaaata ctctccgaga ttcgccgcga gctgccgcag aatggcgcgg gttataaggt
     3481 cgttgccacg ggtggtattt ctgaatcgct cgattttgcg ccggagttct ttgatgtcgt
     3541 ggacaagaac ctgacgctga aagggcttta cctctcggtg tcagacgcat attgagacgc
     3601 gcgtccgaag cgccgtgcca tgacgaaact tcgatacctc acgtttctca aagtccgcac
     3661 actaccgctg ctcgacggct acctgctcaa gcacttcttg ccgtcgctgc tggttgccag
     3721 taccttcttt actttcatcg tgctcgtctt ctttcttaaa gagactataa agcatgcggt
     3781 agaaaaaaat ctcgaattcg gtgtcattct cgaactctgc tggtacgcgc ttggctggac
     3841 actcactctt acggtgccaa tggcatttct gctttcgaca attcttacgg tgggcgcatt
     3901 aaacgccgat tcggagatta ttgccctgcg cgccggcggc atcacgtacc cgcgtatcat
     3961 gcgcccgttt gtgggggttg cggtcgtgtt cttttttatt ctcttttggt tttctaattt
     4021 tgttgtgccg cgtttcgccg acaacatgga aaacatgcgc cagtacattc tgacgaccga
     4081 ccctattgct gcgatacagc caggccagtt tattacgctc gataagcgcg agggtaacgt
     4141 acgtaaaatc tacatcgaac gcgcggtgaa agacgcatcg tttcgcggcg aaattctgca
     4201 gaacatccag gtgcgcaaga tcggcaatgt cggcggcgtg cgccgcctta cagagattat
     4261 catcgcgcag gaaggccgca aggttctcaa gcagacaccg aacggcgaat gggtgaaggc
     4321 gctcaggctg acgcgcggct acctgttcac ctacgggctc gacggaacat tccagcgggt
     4381 agatttctcg ggcggcatgt ttgatctgaa tatacaggag ccggaaagaa tcaagaagat
     4441 gaagcgcatc gaggatatgg gttcgctcac attaccgcag ctgatcggtt cattcgaaac
     4501 gataaagtcc tatcctaagg ccgacgaact ggcccgccgt gcgcacctcg aaatacacaa
     4561 gcgcattgcg ctttctcttt cggtggtgat cttcgttttc atcggctttc cgttggccat
     4621 tgttaaccgc cgcagcggca aaggcatggg acttggcatt tcgatcatct ttattttcgc
     4681 gtactttgcg ctgtttcttt ctgccgaaac tttctcggtg cgccagcagt gggtttcgcc
     4741 ggcgatcgcg gcgcatacgg cgaatttcct gatggcgttg ggtgccggat ggttttcgct
     4801 cacccgcctg gcggagaaga gcctcagctc gattattttc cggaagaggg tgtagcaggc
     4861 tccgaaagcg attccgaaaa agtggcaagc ctcgcagtta gtgcattgca atcatgcggg
     4921 cgaaggtgcg gtaggcgtcg gaatcctgcc gtgtgaattc gagcccgtac ttaaagaagc
     4981 tagccggttc gccgtcgtgc tttttccagc ggatattgcc gcgcaggtcg atgatcttat
     5041 ccgggtaatc ttctgcgaca atccagattg ccatcaactg gccttcgtta tagcggatgt
     5101 gcgactggaa cgaaagcccg ctctgcgaaa tatctatcag atagggcgat tcaaaaagtt
     5161 ccggcgtacc cagttctgtg ttaaaagcag aaatccacat ctggctgata cctttcttgc
     5221 gcgggttctt acgcctgtct ttgagcggtt gctcaaaata agcgagtagc ttctggcgca
     5281 gcaccgggcg cgttacgggt ttttgcacca ggtaggccac ttctttcaaa atgtcagaat
     5341 cgagcgtgcc tgctgcctgc gggtcgtaca gcatgaaaat cggtacatcg gggcagaact
     5401 ggcgaaagac ggcgagcgct acgcgaaacg tgtcatcgat cgcttcaggt gccatgactg
     5461 caagctccgg gggagctttt gtgtctatgg tgcctgcttc ggtgacaacg gaagctccca
     5521 aagttctgag cactctgcct gcaagccggt cacctgattc aaagagatac accgacttac
     5581 ccgacagcgg atgaaatttc agacccgcaa gagctttttc gagcgcctgt cgcgtgaacg
     5641 gtttttccag caggtggtcc gtcaccgaga gcagcgcggt gtcgatctgt ccccgctcgg
     5701 taatttcatg cagcaggact accttgagtg cggaatcatg gcgcttcagt atcggtaatc
     5761 ggtgcgccag acctgcagtg cccgaatagt gcgcgaccag cacattttct gtttcttcat
     5821 tgagatcgag ttcttcaagc cgcgcaataa cctgtgtctc cgcgccacgc tcgcgcaggt
     5881 aagcaacaag cgcgcttgcc accccgggct cttcacccac caggaaaaaa cgcgcctctg
     5941 cgagcggtgc ttcagtcatg attgctcacc gccgcagaac ataaaattaa gtttatcagg
     6001 aaattttgag agcgcttttc actgatgaga tcaaccgctc ttcggtaaac ggcttgatga
     6061 tgaagtcatg agcgccagca gtaacagcct ctttaataaa gcggctttcg ccgagggcag
     6121 aaacgacgat caccttggcg tctggcacag cctttttcac cagcttggtg gcttcgatac
     6181 cgcccaggtt tttcatcgac aggtccatcg taatcaggtc gggttttagg ctttcagcca
     6241 ttgccaccac ttcactgcca tcggtcgctt ctccgacgac ctcgaatccc ttggactcga
     6301 gatgccgccg gataacctgg cgcatgtagc ctgaatcgtc tacgatcagt attcttttct
     6361 tatcggacat atgtttacca cgacgtccgg aaccgcgatt ttacgacttc gctccgcgat
     6421 tttttatcga gatccagatc tcgggaaggt aggcccccac cagtatcatg atcagcccta
     6481 ccaggttggc tcctgacagg aattttaccg attgccagcg gtctgcactc gtattcatga
     6541 tcgagaggta ccagccgccg agcaggagcg caagaccgag taggtgtgcg atggtgtagg
     6601 ttttgatttt ttgccacatg gttcatgatt cactagtcac cccgtgcagc aagcgatttc
     6661 gcgttttggc gggggatttt tcgccaaatg aggagagact cggttacgac cccgacattt
     6721 tcgttccatt tcagacaatc gaactcatag ttagtcaggt gcgtcgccaa cctcttccgt
     6781 ctagccggct tctgccacag tagaatcatc cggtagacca gccacgacac actcacccgc
     6841 atcgcggccg cagctgcgtg caaggtatca tattccgcct gggaaaagcg cgtcgtcacc
     6901 accagatatt cccccggttg gcgattgtag cgtcgcttga cgccccggcg cactccattc
     6961 tgccgcatag ttagtcgcag catccgaacg gccacgcgca taacctgcct ttgccgcctg
     7021 cgccacacgg tcgctgcccc ttttggtaat gaaacacttg taatcagcat aacacctcta
     7081 atatgtaaat accagaaaat cgcgtttttt tctcacttct cacgaattta atcgcagcca
     7141 ggccgaattt tctaacgagt gggatttact tcggtcacgt gcccgaaatc cgccaacaag
     7201 cgattagccg atgattctat ccaataatcc aaaattgtgc gcagcgtgtt gtgcagcgca
     7261 ttagacttgt tgtgcaatga gacataacac tgggctctgg ttcataaaca accattcacg
     7321 gttttccctt tttttggccc ctaaactatc ttgtagcaca tattacagtg ctgagttggg
     7381 agaaatgcgc ctgtggtgcg tctcaaccga ttctgataca tacagaggga caaatgaaaa
     7441 gtgcataccg ttggaaactg ctgctgggtc tcgggctaat tgcctttttg gctgcctgtg
     7501 cgccgaagcg taaagaaacc cagttctcaa aaaacaaaga agctgaagct ggacaaacct
     7561 acacggaaga ccagttacgc gctgctttag gctcggaccg ttacgatgca ctggtaacgg
     7621 gtatcggggc tgccaacctg aacctgctca cctacggtat tggtatctca aacatgacgc
     7681 agatgatcaa cggcatcagc gcaccgggca aacttacctc cctcatgtcc gatggtgcga
     7741 actcacgcaa gcttaacgcg attgaggtac tcgacctgct caacaaactc gacgatgcct
     7801 gccagcgacc tggttctttt ggcacagata ccatcggcaa gatggtgaac atgatcaaca
     7861 atgtgaccct cgctggtatg gaaggtatca agaacatcgt gcacggcgta caggaccagc
     7921 ctgcagacct caatggtaca gcctaccaga acggcgttgc gcgccttggc ttccttgttt
     7981 ccctgcttaa cgaaaacttc agcgtcatgc cgacgctcgt gaacgacctt gcagcaccgg
     8041 gcagcgtttt ctcagcttct ggtaaccaga agctgattcg cctggtaaac agctcgctcg
     8101 atgtgcgcga ccttgctgtc atcattaacg gaacaacgaa cctttcaaac attacggcgg
     8161 tcatggcggg tctcacgggc aacatgtttt gctctaagcc tgagtaccag acacctgcca
     8221 cttgcagcag tggcggtgga acctggaata acacgttgtg cagcgtcggt tcgccgaact
     8281 ctccccacgt gacccgtact gcctgcgcag gggccggtgg cacctggaca cccgacggta
     8341 ttgaaaacat gacgcggctc atcaatgaac tcgatcgtgt ttgttcgaat acttcttata
     8401 caaatggtaa ggcatgtatc gatgccggcg caacctggag aacaaaagcc tccaaaattc
     8461 ctgttatcgt gaacaatatt acgaacgttc ccaacatgtt cacgattgtc aacgggctga
     8521 gcaacgacgg caagcgtcca cagtggcctg tcggtggcgt gaccgtggcc ctgcctgttg
     8581 ggcttggttt gccgacagct tacgctggtg atgccggtaa cgtttttgat ggtgttgact
     8641 cgatggttgc taccatcaac aagctctatg tcactggtgc tggcgatgcc gattcatttg
     8701 gcacagaagg catgaagcgg atggcctacc tcgtgaacaa cctcgacacg actggtgttt
     8761 ttccgaacga tacaaacttt gatgatatca ccggtggatt taactgcgct gtaggaaatt
     8821 ttgacaaccg actgaactgg gctttctcta acaacggtag cgctgctgcg ccatttaatg
     8881 gtcgcagctg ggcatcggac aaca
//
LOCUS       DAOQJF010000111         8891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4206_length_8891_cov_5.520598, whole genome shotgun sequence.
ACCESSION   DAOQJF010000111 DAOQJF010000000
VERSION     DAOQJF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4206_length_8891_cov_5.520598"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLF85_07435"
     CDS             <1..282
                     /locus_tag="PLF85_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19534.1"
                     /translation="VAFVWYFLFHLLRVVSGYKLGLADVRLVLALGFLLGHPFALWLP
                     GGAALLAIVFWLLRRYSVLVYAPSGEQIPFGVFLGVGYLLLNIARAAYH"
     gene            complement(250..1809)
                     /locus_tag="PLF85_07440"
     CDS             complement(250..1809)
                     /locus_tag="PLF85_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE19535.1"
                     /translation="MEFLFIFSLVVYILDIIVLFYYGLHCFVMVKLFFKYRQNCVSDP
                     NRLSDLTKKMKSWPKVTIQLPMFNEYYVAERLIDTTMQVRYPKNRLEVQVLDDSTDET
                     TDLVKKKVAAYKKKGFDIKLIHRTNRQGHKAGALREAQEVAKGEFIAIFDADFLPAED
                     FLEKTVPYFYESDDIGMVQTRWGHINADYSILTKGQSLGIDGHFTIEQIARGGSGLWM
                     NFNGTAGIWRKTCIYDAGNWSADTLTEDFDLSYRAELKGWRFKYIVDVVNPAELPATV
                     NAYKSQQFRWCKGSIQTTVKLAPTIFRTDLPWKVKLEALTHLTNYLVHPLMVINILAT
                     LPVLYFSERLGNLPDAIIGIAAVVFSLGTFGPIAMYLVSQKVLYNRWANRIAWMPILT
                     MIGTGIAVSNTRAVWQALTGKKSSFIRTPKLRIEGQKDKVAERMKYAGNVKLDKTPFL
                     EFFMGIYAVMIIYTAFVFERTMFVPFMVMYACGFFYISIASFWENFQEVRAKKRLAMA
                     ASMVSGTGNIQ"
     gene            1972..2394
                     /locus_tag="PLF85_07445"
     CDS             1972..2394
                     /locus_tag="PLF85_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNE19536.1"
                     /translation="MRAKLAIFFVLLAGALVILVVTSSTSAAQVHLKLHEFRAEADRA
                     GLEKLASRNLTLYGKVKEGSIEREGVRARFTIVDQGIEMAVEHSGKTLLPDTFQDGAD
                     AGVEGTYDPARKVFVSHKVMAKCASRYEAPGQMNQKSY"
     gene            2557..5031
                     /locus_tag="PLF85_07450"
     CDS             2557..5031
                     /locus_tag="PLF85_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19537.1"
                     /translation="MTRIPYRPQKAILGGALLLVLGGAAYFSLRQSDPLKGFDPAGIV
                     DERDARVQSLLELLKLDNEDFRTHAELAKLYFDLGELDNAERHALSAIAVGRRKNADR
                     EFMTNQYLLLSKIYQAKGDQQKALEYASRAAESDPTKTAPLKRKGQVFEAQRKNDKAR
                     LEYLKALKLDEKDPETYALLAAQEFKKGNRKAAMEWLKMGVRRNPTNAIAFRNLARGY
                     VRTGELQKGREAYERALALDPNNAGTRHEYAKLLKKMGERDRYVAELRKAHNSDPKNP
                     KILADLGDVELAAGNKQAALSHYRDALKRDGKNAKLREKYNTLYNELLAEQGKKSSGP
                     AVAVTGQDGAGAGSGSETAGSGTAKTVAEGGNGKEKSDGGAASGKSQEPPDAGLSSGS
                     KNNEPASQGITKELEAGKKAFAEKDYGGAEDYFRRALEKSPDSQEARFFLARSLDAQG
                     KKDAAMAEYRKVLAKEPEHAKANYYLGRLLYQAQKFPEAERAFQKSVNSDGQFGPARY
                     SLALSQEKQGKNNEALAAYRKAAETDSSLTQAHFNSAILLKKSKRYDEALAELAKSGK
                     GSDVEYQRGEILLKQKKYAEAKDALARVLSEKPQHYEAAFNLALAHHKLGDPAGADKV
                     LSRVIRDDSPADLHYTYGKLLEESGEMAGAEKQYRVSVQKDARYFKGWLNLGRVTAQG
                     QKFDQAEHAYRQALLVEPASYEANFNLANALFKQKKYQTAIEYFENARRNESGREVVL
                     PLASSYENTSQPEKAAKVYADFLKENPKDRVVLERLGYLYYRKTKEKDKALEQFNKLL
                     KYYPDSEKAQEYKGMVQLIEKQKTEQ"
     gene            5041..7077
                     /locus_tag="PLF85_07455"
     CDS             5041..7077
                     /locus_tag="PLF85_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNE19538.1"
                     /translation="MASAADELRKIILALDNEDELREQERTKPPREVIVVADSVKQAL
                     SLAAAQMQKDEFQLDYKILNRGGSQLFGLRRLPYRVHVFPAREDTRFSDLEDMNVSLS
                     GAIAQAALADDAPIAINSDGKILVRIYRTGVYVIVRPPVGEGQPVTVTQAMERLRTAG
                     VTAYDKGRLENEVKARSAKQVKIAEWTPRPDADSQLQIEVSADHMRASMTVSRPRPGG
                     RHLQVADVMQALKANGVQFGFKEPTIEKALDEEVYSTPVVVAEGKQAQNGRDGYIDYK
                     VRIDKKVEFKEDESGRVDFLQRDLVENVIQGQVLAELVPAQRGQEGRDLFNKILPAKN
                     GNPVTLRPGPGTMLSDDGKKLLAEKNGQVIYRGGILSVHDMFTVNGDVGITTGNITFL
                     GSIMVTGNIEDNMHVKAAGGIEVAGTIQKAYVEAEGDIIVRQGIIGRDEAQVESTAGS
                     VYGKFLQNGVCMAEQDVVMGENIMHSRVYAGGNVVCNGKRAQLVGGEIICGGVVRVKS
                     LGAMAATPTHVMAGVNPRAMQQLKQIAQIEKQLREKISQTEVNLKTLQQQKAAAPDAF
                     SEAKEEQLVKTIAFRDKLNERLREALSEKENIETFMKDAQSEGAVHVEKTAFPGVTIE
                     INGASFTVQDEYNNVSFVEEAGRINIVAYIAPGDDDKHFQKSRRKGRYSETPRS"
     gene            7142..7429
                     /locus_tag="PLF85_07460"
     CDS             7142..7429
                     /locus_tag="PLF85_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19539.1"
                     /translation="MLNRLSGEDSMREQRLRDSQVNQPEAKVMHEDMFSAEAYEAPDY
                     AEQGGSGKKKQGESHAAAEEVKPSAEEAATEKDKTEPAAGESHTPGFSTYA"
     gene            complement(7416..8279)
                     /locus_tag="PLF85_07465"
     CDS             complement(7416..8279)
                     /locus_tag="PLF85_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNE19540.1"
                     /translation="MKVFIWGMGLIGASLGLRLKQAGYQVSGAVRSERSSSILQQMGF
                     SDIVTDTESCLRTLAECDMVALGLNIEDCFPAIDHVMEVPALRDRLILLDMCSSKKQI
                     CDYMQQKYPDAAFVGCHPMAGKEKQGPEAAEATLFEGSTVFLAAPVAEPSVRQRAARE
                     AVAELWAATGARTAVVAPDEHDRIMAHVSHGLHLAACLIARLSGRLNTEALAASPAAG
                     SYRDMTRIAESSGVMWNEITASNRDHVTAWLRDLAAEANRLADDLDAGKADIAALFAE
                     ASAARSRVMRK"
     gene            complement(8276..>8891)
                     /locus_tag="PLF85_07470"
     CDS             complement(8276..>8891)
                     /locus_tag="PLF85_07470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE19541.1"
                     /translation="DRITANLDELQKIGEDYYQTIFQTAPDLGDIASTDSAKQQSAKI
                     LPLMSVMQIITAREEAYISMPIGIAVRSNQITSLYGRRIDPFGLETNFHTGIDFAGGV
                     GAPIYATADGVVASAADEGTTGLGKNVRLEHKFGIITVYAHMNEISVRKDQKVRRGEL
                     IGFVGTTGRSTGPHLHYEVRVRSLEPDNYYELTYNPMPFIREKL"
BASE COUNT         2130 a         2358 c         2580 g         1823 t
ORIGIN      
        1 gttgcgtttg tctggtattt cctgtttcac ctcttgcggg tggtttcggg ctataagctg
       61 ggcctggcgg acgtcaggct ggtactggcg ctgggattct tgctgggcca cccatttgcc
      121 ctctggttac ccgggggggc cgcgttactg gctattgtct tctggctgct gcggagatac
      181 tccgttctgg tctatgctcc ctcgggggag cagataccgt tcggagtttt cctgggagtg
      241 ggttacctgt tactgaatat tgcccgtgcc gcttaccatt gaagcggcca ttgccaggcg
      301 ctttttcgcg cgcacttcct ggaagttttc ccagaatgaa gcgatgctga tgtagaagaa
      361 gccgcaggca tacatgacca taaacggcac aaacatggta cgctcaaaga cgaacgccgt
      421 gtagatgatc atgaccgcgt agatgcccat gaagaattcg agaaacgggg ttttgtcgag
      481 tttcacgttg cccgcgtact tcatgcgctc cgcaaccttg tctttctggc cttcgatgcg
      541 cagcttcggc gtacggatga acgaagactt tttaccggtc agcgcctgcc atactgcgcg
      601 cgtattagag acagcaattc ccgtaccgat catcgtgagt atcggcatcc aggcgatgcg
      661 gtttgcccag cggttataga gcaccttctg tgacacgagg tacatggcga tcggcccaaa
      721 ggttccaagc gaaaagacga ccgcggcgat accgatgatt gcgtcgggca ggttacccag
      781 gcgttcgctg aaatagagca ccggcagcgt tgccaggatg ttgatgacca tcagcgggtg
      841 tacgaggtag ttggtgaggt gcgtgagcgc ttcgagcttt accttccatg gcaggtcggt
      901 gcggaaaatt gtcggggcaa gtttaactgt agtctggatc gagcccttgc accagcggaa
      961 ctgctgcgat ttgtaggcat tgacagtagc gggaagctcc gccgggttca cgacgtcgac
     1021 gatgtactta aagcgccagc ccttcagctc agcgcggtac gacaggtcaa agtcttcggt
     1081 cagcgtatca gcagaccagt tgcccgcgtc gtagatgcag gtcttgcgcc agataccagc
     1141 cgtgccattg aagttcatcc agaggccgct gccgccgcgt gcgatctgct cgatagtgaa
     1201 gtggccgtcg atacccaggc tctgtccctt ggttaggatg ctgtaatcag cgttgatatg
     1261 cccccagcgg gtctgtacca tgccgatatc gtctgactcg taaaagtagg ggactgtctt
     1321 ttcgagaaaa tcttcggctg gcaggaagtc ggcgtcaaat atggcaatga attcgccttt
     1381 cgcgacttcc tgtgcttcgc gcagtgcccc ggccttatgg ccttgccggt tcgtgcggtg
     1441 gatgagtttg atatcaaaac cttttttctt gtaggcggct actttctttt ttaccaggtc
     1501 ggtggtctcg tcagtcgaat catccagcac ctggacttcg agccggttct tagggtaacg
     1561 tacctgcatc gtggtgtcga tcaggcgctc ggccacgtag tactcgttga acatcggcag
     1621 ctggatcgtg accttcggcc agctcttcat ttttttcgtg aggtcggaaa gtctgtttgg
     1681 gtcagaaacg cagttttggc ggtacttgaa gaacagcttc accatgacga agcagtgcaa
     1741 accgtagtaa aaaaggacaa ttatatctaa aatatagact acgaggctaa aaatgaacag
     1801 aaattccatg gatggtgcag agtgcaattt tgagacaggt ggtcaatgaa agctccgtgc
     1861 cccgaaaaaa tgcatgggat tttttttggc accggctttt tgtgcaagcg cagtcaaggc
     1921 ttcacacttg ctttaacttg ccgaagcagt cttgcgtgcg tatcatgaag tatgcgggca
     1981 aagctggcaa tctttttcgt actccttgca ggagctctgg tgattctggt agttacttct
     2041 tcaacctcgg cagcgcaggt gcacctgaag ctgcatgaat tccgcgcgga ggcagaccgt
     2101 gccggtctgg agaaacttgc cagccgcaac ctgaccctct acggcaaggt gaaagagggg
     2161 agcatagaac gcgaaggcgt ccgcgcgcgt ttcacgattg tagatcaggg tatcgagatg
     2221 gcagtcgagc actcgggcaa gacgctcctg cctgatacct ttcaggacgg tgctgacgct
     2281 ggcgtagaag gtacttacga tccagcgcgc aaggtctttg tctctcacaa ggtcatggca
     2341 aaatgcgcct cgcgctatga agccccgggg caaatgaacc agaaatccta ctagcaggtt
     2401 ccggaaaaac acccgcaccg gctccttttt caggagcctg ttagtagtaa acgagccgtt
     2461 tactaacgcc tctgtccgct cagcgtgaca acaaagacgc ctgcccccag cggccaaact
     2521 tcgcttcagt ctctggcaaa ttgccgataa tagaccatga cccgcattcc gtaccgaccg
     2581 cagaaagcta ttctcggggg tgccctgctg ctcgtgctcg gtggtgcggc gtatttcagc
     2641 ctgcgccaaa gtgacccgct gaagggtttt gatccggcgg gtattgtcga cgagcgcgac
     2701 gcacgggtgc agtcgctgct cgaattgttg aaactcgata acgaagattt ccgcactcac
     2761 gcagaactgg caaaactcta ttttgacctc ggcgagctcg ataacgccga gcgccatgca
     2821 ctgtcggcca tcgccgtggg tcgccgcaag aacgccgacc gtgagttcat gaccaaccag
     2881 tacctgctgc tgtcaaagat ctaccaggca aagggcgacc agcagaaggc gctggagtat
     2941 gcatcgcgcg cggcagagag tgatcccaca aaaaccgctc ccctgaaacg caaaggacag
     3001 gtttttgaag cacagcgcaa gaacgacaag gcgcggctcg agtacctgaa agcgctcaag
     3061 ctcgatgaaa aagatcctga aacatacgct ctgctcgctg cgcaggaatt caaaaaaggc
     3121 aaccgcaagg ccgccatgga gtggctgaaa atgggagttc gtcgcaaccc gacgaatgcc
     3181 attgcgttcc gcaacctggc gcgcggttat gtgcgcaccg gcgaactgca gaaggggcgc
     3241 gaggcatatg aaagagcgct ggcgcttgat ccgaataatg ccggtacaag gcacgagtac
     3301 gctaagctgc tgaagaagat gggggagcgc gatcgttatg tggctgagtt gcgcaaagcc
     3361 cacaactccg atccgaaaaa tccgaaaata ctcgccgacc tcggcgatgt agaacttgct
     3421 gccggcaaca agcaggcagc gctgtcacat taccgcgacg cgctgaaacg cgatggcaag
     3481 aacgcaaaac tgagagaaaa gtataacact ctctacaatg aattgctcgc agagcagggt
     3541 aaaaaatctt cagggccggc agtggccgtc acgggtcagg atggagctgg agcaggcagc
     3601 ggcagcgaaa ctgccggcag cggcacggcg aagaccgtgg cagagggcgg taacggtaaa
     3661 gaaaaatcag acggtggagc agcctctggt aagagccagg agccgcctga cgccgggctt
     3721 tccagcggtt cgaaaaacaa cgagccagca tcgcagggaa taacaaaaga acttgaggcg
     3781 ggcaagaagg ccttcgcgga aaaagattac ggcggggcag aagattattt tcgccgtgca
     3841 ctggaaaaat cgcccgattc acaggaagcg cggttcttcc tggcgcgcag tcttgacgcg
     3901 cagggaaaaa aagacgccgc aatggctgaa taccgcaagg tactggcaaa agagccggaa
     3961 catgccaagg caaactacta cctcggccgg ctactctacc aggcgcagaa atttccagaa
     4021 gctgaacgcg ccttccagaa gtcggtgaac tcagacggtc aattcggccc tgcccggtac
     4081 tcgcttgcac tttcccagga gaaacagggc aagaacaatg aggcactggc agcctatcgc
     4141 aaggcagccg aaaccgacag tagcctgacg caggcacatt ttaacagcgc tatcctgctc
     4201 aaaaagagca agcgctacga cgaagcgctg gcagaacttg ctaaaagcgg caaaggcagc
     4261 gatgtggaat accagcgcgg ggaaatactg ctgaaacaaa aaaagtatgc agaagccaaa
     4321 gatgcgctgg cccgtgtctt gagcgagaaa ccgcagcact acgaagctgc gttcaatctg
     4381 gcgctcgcgc accataaact cggcgatccg gccggggccg acaaggtgct ctcgcgcgtc
     4441 atacgtgacg attcgccggc agacctgcat tatacctacg gcaaactgct tgaagagagc
     4501 ggtgaaatgg ctggtgccga gaagcagtac cgggtaagcg tgcaaaagga tgcgcgctac
     4561 ttcaagggat ggctgaacct tggtcgggta acggcgcagg gacagaaatt tgaccaggca
     4621 gagcatgcct accggcaggc cttgctggta gaacctgcat cttacgaggc caattttaac
     4681 ctggcaaacg ccctcttcaa gcagaagaaa taccagactg cgattgagta ctttgagaat
     4741 gcgcggcgca acgaatcagg ccgtgaggta gtgctgccgc tcgcgtccag ctatgagaat
     4801 acttcgcagc cggaaaaagc ggcaaaggta tatgccgatt tcctcaaaga gaatcctaaa
     4861 gaccgtgtcg tcctcgaacg cctcggttat ctctattacc gtaagacgaa agaaaaagat
     4921 aaggcgctcg aacagttcaa taagttgttg aaatactatc ctgattcgga gaaggcgcag
     4981 gaatacaagg gtatggtaca gctgatcgaa aaacaaaaga cagagcagta acagcagaat
     5041 atggcatccg cggcagacga actcagaaag ataattctcg cgctcgacaa tgaagacgag
     5101 ctgcgcgagc aggagcgcac aaaacctccg cgcgaggtca tcgtcgtcgc agattcggta
     5161 aagcaggcgc tgtcgctcgc cgccgcacag atgcagaaag acgagttcca gctcgattat
     5221 aaaattctca accggggtgg ttcgcagctt ttcggtttgc gtcgcttgcc gtaccgggtg
     5281 catgttttcc cggcacggga agatacacgc tttagcgatc ttgaagacat gaatgtctcc
     5341 ctttccggcg ctattgcaca ggctgcgctg gctgacgacg ctcccattgc catcaattct
     5401 gatggtaaga ttttggtccg tatctatcgt accggggttt acgtcatagt cagaccgccg
     5461 gtcggtgagg gccagcctgt caccgtgaca caggcaatgg agcgattgag aactgccggt
     5521 gtcacagcgt atgataaggg ccgcctggaa aatgaagtca aggcgcgttc cgccaagcag
     5581 gtgaagatcg ccgaatggac tccgcgacca gacgcagact cgcagctgca gatcgaggtg
     5641 tcagccgatc atatgcgcgc gagcatgacc gtctcgcgcc cgcgtcccgg cggacgccac
     5701 ttgcaggtgg ccgatgttat gcaggcgctc aaagccaacg gcgtacagtt tggcttcaag
     5761 gagcctacga tcgaaaaggc tctcgacgaa gaggtttatt cgacacccgt cgtggtagcc
     5821 gaaggtaagc aggcgcagaa cgggcgcgac ggctatattg attacaaggt tcgcatcgac
     5881 aagaaagtcg agttcaagga agacgagtcg ggccgcgttg actttctgca gcgcgacctg
     5941 gtggaaaacg ttatccaggg tcaggtgctt gctgaactgg tacccgcgca gcgcgggcag
     6001 gaagggcgcg acctttttaa caagattctg ccggctaaga atggaaaccc cgttacactg
     6061 cgaccgggcc cgggcaccat gctgtctgat gacggtaaga agctgctggc tgagaaaaat
     6121 ggtcaggtta tctaccgtgg cggaattctc agcgtccacg acatgtttac cgtcaacggc
     6181 gatgtgggta ttacgacagg taatattact tttcttggtt caatcatggt gaccggcaac
     6241 atagaagaca acatgcacgt caaggcggcg ggtggaatcg aagtagcggg tacgatccag
     6301 aaggcctatg tcgaggctga aggtgacatt attgttcgtc agggtattat cggtcgtgac
     6361 gaagcgcagg tcgaatcgac ggcgggttct gtttacggca agttcctgca gaacggcgtg
     6421 tgcatggccg agcaggatgt ggtcatgggc gagaacatca tgcactcccg cgtctatgcc
     6481 ggaggcaacg tcgtctgcaa cggtaagcgc gcgcagctgg tcggaggaga aattatctgc
     6541 gggggagttg tgcgcgtcaa gtcgctcggc gcgatggctg ccaccccaac tcatgtcatg
     6601 gccggggtga acccgcgcgc gatgcagcag ctcaagcaga ttgcgcagat tgaaaagcag
     6661 ctgcgcgaga aaatttcgca gacggaagtt aacctcaaga cgctgcagca gcaaaaagcc
     6721 gcagcgcccg atgcattcag cgaagcgaaa gaagaacagc tggtgaaaac gattgccttt
     6781 cgcgacaaac tcaacgaacg cctgcgtgaa gcattgagcg aaaaagagaa catcgaaaca
     6841 ttcatgaaag atgcacagtc cgaaggtgcc gtgcacgtgg aaaaaacagc ctttccagga
     6901 gtaaccatcg agatcaacgg tgcctcattc accgtgcagg atgaatataa caatgtgagc
     6961 ttcgtggaag aagccggccg catcaatatt gtagcctaca tcgcacccgg cgacgacgac
     7021 aaacacttcc agaaatcacg gcgcaagggc aggtacagtg aaacccctcg atcttaatgt
     7081 cagcatacaa aattcctatg aggcggcccg cgtagagtcg gtacgcctcg aaaaggaaca
     7141 cgtgttaaat cgcctctcag gcgaagactc gatgcgcgag cagcgcctgc gcgattcgca
     7201 ggtgaaccag cccgaggcga aggtcatgca cgaagacatg ttctccgccg aggcgtacga
     7261 ggcccccgac tatgcggagc agggtggttc aggaaaaaag aaacagggtg aatcacacgc
     7321 tgcagccgaa gaggtgaaac catcagctga agaagctgct acagaaaagg ataaaacaga
     7381 acctgcggct ggcgaaagcc acaccccagg attttctact tacgcataac acggctgcgg
     7441 gcggcgctcg cctcggcaaa cagcgccgca atatcggctt tccctgcatc caggtcatct
     7501 gcaagacgat ttgcctctgc tgcgaggtcg cgcagccatg cagtcacgtg gtcccggttg
     7561 ctcgcggtaa tttcgttcca cataacgccc gaactttctg caatccgcgt catgtcgcgg
     7621 tagctacctg cagcaggcga agctgccagt gcttcagtat tcagtctgcc gctcagccgc
     7681 gcgatcaggc aggctgccag gtgcaggccg tggctgacgt gtgccatgat acggtcgtgt
     7741 tcatcgggcg cgaccacggc tgtgcgcgcg cccgtggccg cccataactc cgctactgcc
     7801 tcgcgtgcgg cccgctgccg gaccgagggt tctgcaacgg gggctgccag gaatacggtg
     7861 gatccttcga agagcgtagc ttctgccgcc tcgggaccct gtttttcttt gcctgccatg
     7921 ggatggcacc ccacgaaggc cgcatcggga tatttctgct gcatgtagtc gcagatctgc
     7981 tttttgctcg agcacatgtc gagtaggatc aacctgtcgc gcaaggccgg tacttccatg
     8041 acgtggtcaa tcgccgggaa acagtcttca atattcagac ccagtgccac catgtcacat
     8101 tctgctaggg tacgcaggca actttcagta tctgtcacga tgtccgagaa gcccatctgc
     8161 tgcagaatgc tgctactgcg ttcgcttcga acagcgccgc tgacctgata accggcttgt
     8221 ttcaggcgca ggccaagcga ggccccgatg agacccatac cccagatgaa taccttcata
     8281 gcttctcgcg tatgaacggc atcgggttgt acgtcagttc gtagtagttg tcgggttcga
     8341 gactgcggac acgcacttcg tagtgcaggt gcggcccggt agagcggcct gtcgtgccga
     8401 caaaaccgat gagttccccg cgccgtactt tctggtcctt gcgcacagaa atttcattca
     8461 tatgcgcgta gacggtgatg ataccgaact tgtgctcgag gcgcacgttc ttgccgaggc
     8521 ctgttgtacc ttcatcggca gcgctcgcca cgacgccatc agctgtagca tagatgggag
     8581 ctcccacacc gcctgcaaaa tcaatgcctg tatggaaatt tgtctcgagg ccaaacgggt
     8641 caatgcgacg gccatagagc gaagtgatct ggttggagcg cacggcgatg ccgatcggca
     8701 tgctgatgta ggcttcttca cgtgccgtaa tgatctgcat gacgctcatc agcggcagga
     8761 ttttcgcgct ctgttgcttc gctgagtccg tcgatgcgat gtcgccgaga tccggcgcgg
     8821 tttgaaaaat agtctggtag tagtcttcac cgatcttctg taattcgtcg agatttgccg
     8881 tgatgcggtc t
//
LOCUS       DAOQJF010000112         8789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4266_length_8789_cov_5.554729, whole genome shotgun sequence.
ACCESSION   DAOQJF010000112 DAOQJF010000000
VERSION     DAOQJF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4266_length_8789_cov_5.554729"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLF85_07475"
     CDS             complement(<1..233)
                     /locus_tag="PLF85_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19542.1"
                     /translation="MRESAPIDPRLKAAIDNAIANEHLVRIRAYAVISATEAGLSYIV
                     EQILAKYNRPDMVGPVYTSVKELSLNGAKANFKR"
     gene            340..822
                     /locus_tag="PLF85_07480"
     CDS             340..822
                     /locus_tag="PLF85_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19543.1"
                     /translation="MAEGKKPGVSHQDTYTENEVLARIESILKFTTDELQNTEVRAQA
                     FEKLSEFEAQLLYQQRHVSTALENVQKYRDEIEKEWNKEKQVRENTLDLMAQRMKELH
                     QTLRAQELLLDYAAAEQKFLRELLIEIHDKKKLTEADGERILAAHEKLLGELSRKAKK
                     "
     gene            830..3217
                     /locus_tag="PLF85_07485"
     CDS             830..3217
                     /locus_tag="PLF85_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNE19544.1"
                     /translation="MPRPTVQVRFRALGVALNRYLAQPRSFAEIKKATLISYGFTLLL
                     SVLFLALPVMQKIPRFNAGDVVQTDLKALMDLRIEDEAETERLRKAAYERERPAFDRD
                     YVITEKILQQLKTDFTWIARTIAETRNTPLSDRQALLTDRMPWLEGSPYRKPDIEALL
                     NEKKTEILEPRTLQIAEKVFSESGFLRTPPDAAVTGEMMEKGAQVRTINHPRDLPDVV
                     WSAEQVQTAEATAKLALRESQLKDAELSRGTMRIVLTRIRELMRENPALVYNAQYTEL
                     RRKQAANRVTPVYRPIKRGTILFRAGDVIDDEKLRLLDQVRENHRRRNGSQLLGILFV
                     MGVLAVSIAYFTFRFAWEQVRDYGSHIILHGLFAFMFMLELFIMVVNPLRNYEVNFVL
                     FVPFGFFGILTGQFFGARIALSAGIYLSIFSFILTGFDRESLLLALTTAIAGLYASTR
                     MHKRSQMFKGGLIIAVTNMVLITGFELLAPAARNFELKVGAIAVNSILSILLTLGILP
                     LLEFLFNLPTPFRLMELNDFNHPLLTRMAAIAPSTHSHSVMLANLSEAAVRALGGDTL
                     LTRVGCLYHDIGKMVHPDFYAENRHLYPTSETFKKLGPQKSAQMIISHVTDGIAMARE
                     SRLPEKVISFIPEHHGTTTIQYFYHKALEAQSARSSHAHVTRSHFQYPGPRPRTKETA
                     VAMIADSVEAASRTASTASPEEFAAIIDRIIQNKISEEQFHEAPLTLGDLSTIRKAFL
                     DVLVSTYHERPQYPTMQETKALEVSVQSMPEKRSVARGKQQRKQKRSAALPQLID"
     gene            3219..4235
                     /locus_tag="PLF85_07490"
     CDS             3219..4235
                     /locus_tag="PLF85_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19545.1"
                     /translation="MTAIFRRNIQILRDGYRQADQPTRRRHVILFALNFLTLFLAGAN
                     FSAHSQAAGRNFDALVFALALSVILLGYSLARFIQARSYGLYSTLPFFIPMPLFSPFG
                     TLGVVTRTAHNGAGSRALFDVAFWGPATSFLLSVPCLVAGTLLTDVVAGVPQFENPLL
                     LRGIAKVLKEIPHGYDLAVHPLLAAGWAGLFFTAINLFPLGNLAGGQIAYTLFGQRQR
                     DIAYIFMAILFVMALWYPMWFAFVLIFIYLGIEHPELRQNRNPFFQEMPQAAIRQPLD
                     RARQYLAAACALVFALSFTMRPFSSTLERVEQRPPAELVPPDHYGTPPAPQEQAEPAE
                     ENSI"
     gene            4272..5945
                     /gene="ettA"
                     /locus_tag="PLF85_07495"
     CDS             4272..5945
                     /gene="ettA"
                     /locus_tag="PLF85_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNE19546.1"
                     /translation="MADDKKIIFSMVGVSKVYPPHKQVLKDIYLSFYYGAKIGIIGLN
                     GSGKSSLLKIIAGVLKPDKGEVVFSPGYSVGYLEQEPQLDESKTVRQIVEEGAGEVVA
                     LLKEYEAVSESLGTDLSDDDMEKAIERQGELGELIEKANGWELDNMLKRAMDALNCPE
                     ETAVIKNLSGGEKRRVALCRLLLQKPQILLLDEPTNHLDAESVQWLEQHLKEYEGTVI
                     SITHDRYFLDNIAGWILELDRGEGIPWKGNYSSWLEQKSNRLANEEKAESKRRKTLER
                     ELEWVRMGAKGRQAKSKARLGAYEKLLNEDAKEKESKLEIYIPDGPRLGDKVIIAENV
                     SKSFGDKLLYENLNFELPPNGIVGIIGPNGAGKTTLFRLIMGLEKPDVGNFTIGETVK
                     ISYVDQQHSAIDPNKTVFEVISGGAPEVKVGKLSVNARAYIARFNFSGADQEKKCGVL
                     SGGERNRLHMALALKEGGNVLLLDEPTNDIDVNTVRALEEGLEAFAGCAVIISHDRWF
                     LDRVATHILAFEGDAQVRFFEGGFSEYEADRKKRMGGEPKRFRYKKLVK"
     gene            complement(5950..6078)
                     /locus_tag="PLF85_07500"
     CDS             complement(5950..6078)
                     /locus_tag="PLF85_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19547.1"
                     /translation="MEILAFEDEPVQQEILSRIFAGRFFASEPEALKASEATPEEA"
     gene            6210..8771
                     /locus_tag="PLF85_07505"
     CDS             6210..8771
                     /locus_tag="PLF85_07505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19548.1"
                     /translation="MFGHIASLAFFFALTLPLASAKNLIREDVYVRPGFDQAWLKSWP
                     ERTKSAGDWRHVPSAAADNRPVKIATLGAPFPEPGIYRWPVEKIHDYTAVTRFFLTPE
                     EKKSLHAWGLRLGSIADNWQIFLNGTLIAQAWHADAANSKITVHRAVRDAVFELPAAL
                     LVPGENLLAFRLAGDAGSAEIGFFLGQPYEVDFLRNLLQKRSETVVLILICLYLAVGF
                     YHLLLYSRRQQESYNLYFALFCIGLFIYLFTRTSVVFDIFADSTLIQKVEYIVLFNVL
                     TPFLVFIDKLFYTRVTLFPKIYGVFALLLSIAVVPTTSHFNTTILRTWQLTALLGILY
                     FLAFQLVPVVVREVRSRYAASQRSPAILRFFSSMQGTLLRSPAGNLLIGILTIVSTAV
                     FDILDSIFFSTGIAFTKYGFAMFVLGIAVMLANRFLQVHNEVEELNATLEKKVEERTR
                     ELQESLVRVQDLKNQQDADYFLTALLLKPLAANTAHSQSIHVEYLIKQKKTFEFRKWK
                     SEIGGDINMASSISLKDKPYVVFVNADAMGKSMQGAGGALVLGAVFQSTIERMRWTRE
                     LSDVFPEIWLHTTFLELHRIFESFEGSMLISLVIGLIDESNGLMYYINAEHPSIALLR
                     NGEASFLDAHILRKLGTPGIHGSLKIHCVQLEPGDVLVMGSDGRDDVILGHNPDGSNI
                     MNENEQLFLEHVTAARGDLQEIFNRVQQTGEIMDDLSLLKIEYNPAEKNAAPADLQQL
                     AELREQAAQLIARDKHAEAAEVYMRVYELDPQDIETCYLIAQCLRREKKIKQALQWAE
                     RCVLRDGENLKYLLQAAELNIMMKRFTKAGSFIRRVKELEPEHQRAAKLETMIHQ"
BASE COUNT         2092 a         2488 c         2306 g         1903 t
ORIGIN      
        1 cgcttgaaat ttgccttagc tccgttcagc gaaagctctt taaccgaggt gtagaccggc
       61 ccaaccatgt ccggccgatt atacttggcg agtatctgct ctacaatata gctaagtccg
      121 gcttcggtgg cagaaatcac cgcgtaggcc cgaatacgta ccaggtgttc attggcaata
      181 gcattgtcta ttgctgcctt cagccttggg tcaattggag cgctttcccg catatatgga
      241 gaaaacgcgc ataagacaag gtttagccct ttgccgactg cgcgttcgcg aactttctta
      301 ttttttttga cctcacgtca atactttgga acatcgtgca tggccgaggg caaaaagcct
      361 ggggtttccc accaggacac ctatacagaa aacgaagttc tcgcgcgcat cgagagcatt
      421 ctaaagttca caaccgatga actgcagaat accgaagtaa gagcccaggc ctttgaaaaa
      481 ctgagcgaat ttgaagccca gctgctttac cagcagcgtc acgtcagtac tgcgctggaa
      541 aatgtgcaga aataccgcga cgagatagaa aaagaatgga ataaagaaaa gcaggtacgg
      601 gaaaacaccc tggatctgat ggcgcagcgc atgaaagaat tgcaccagac tctgcgcgcg
      661 caggagctcc tgctcgatta cgcggccgcc gagcaaaaat tcctgcgcga actactaata
      721 gagatccacg ataagaagaa gctcaccgag gccgatgggg agcgaattct tgctgcacac
      781 gaaaagctgc tcggcgaact aagtcgcaaa gcaaagaaat aagtcgctca tgccgcgccc
      841 taccgtccag gtacgtttca gggcgctcgg cgtagccctc aaccgttacc ttgcacagcc
      901 acggtccttt gccgaaatta aaaaagccac cctgatcagc tatggcttca cattactact
      961 ctccgtcctg tttctcgcgc tgccggtaat gcagaagatt ccccgtttca atgcgggtga
     1021 tgtggtgcag actgacctaa aggcactgat ggatctgcgc atcgaggacg aggccgaaac
     1081 agaacggttg cgcaaggcgg cttacgaacg cgaacggcct gcctttgacc gcgattacgt
     1141 aattactgaa aagatcctgc agcagctgaa aaccgatttc acctggattg cacgcaccat
     1201 cgccgaaacg cgcaacactc cgctttctga tcgacaggcg ttgttgacag accggatgcc
     1261 ctggctcgaa ggctcgccct accgcaagcc agatatcgag gcactactca acgaaaagaa
     1321 aactgagatt ctcgaacctc gcaccctgca gattgctgag aaggtttttt ccgagagcgg
     1381 tttcctgcgc acaccgcccg atgcggccgt caccggcgaa atgatggaga aaggtgcaca
     1441 ggtacgcacc atcaaccacc cacgcgacct gcctgacgtc gtgtggagcg ccgaacaggt
     1501 gcagacagcc gaagcaacgg cgaaattggc cctgcgtgaa agccagctaa aggacgcaga
     1561 gctttcgcgc ggcacgatgc gcattgtgct gactcgcatc cgtgagctca tgcgcgaaaa
     1621 cccggcactc gtttataatg cacagtatac tgagctgcga cgtaaacagg ctgcgaaccg
     1681 cgtcacgcct gtttatcgtc ctatcaagcg cggcacgata ctcttcaggg cgggtgatgt
     1741 aatcgacgat gaaaaacttc gcctgctcga ccaggtgcgt gagaaccatc gccggcgcaa
     1801 cggctcacaa ctgctcggca ttctgtttgt catgggggtt ctcgccgtct ctattgccta
     1861 tttcactttt cgattcgcgt gggaacaggt gcgtgactat ggatcgcata ttatcctgca
     1921 cggcctgttc gcattcatgt tcatgctcga actgtttatc atggtggtta acccgctcag
     1981 aaattacgag gtcaactttg ttctttttgt gccgtttggc tttttcggta ttttaaccgg
     2041 gcagttcttc ggggcacgca tcgcgctgtc ggcgggaatc tacctttcga tcttttcatt
     2101 tattcttacg ggtttcgacc gggaaagcct gctgctcgcg ctcaccaccg caatcgccgg
     2161 cctttatgct tcgacccgca tgcacaaacg cagccagatg ttcaaaggtg gcctgattat
     2221 tgccgtgacg aacatggtgt tgatcaccgg cttcgagctt ttggcacccg cagcgcgaaa
     2281 cttcgaactc aaggtcgggg caatcgcggt caacagtata ctcagcatac tgctgaccct
     2341 cggtatactg ccgctgctcg aatttctttt caacctgcca acgcccttca ggctcatgga
     2401 gctcaacgac ttcaaccacc ctctgctgac acgcatggcg gcgattgcac cttctacaca
     2461 ctcgcacagc gtcatgctgg ccaatttaag tgaagcggcc gtgcgtgcac tgggcggaga
     2521 cacgctactg acgcgcgtgg gctgcctgta ccatgacatc ggtaagatgg ttcacccgga
     2581 tttttacgcc gagaaccgtc acctctaccc gacctcagag acgttcaaga aactgggtcc
     2641 gcaaaaatcc gcgcagatga ttatcagcca tgtcaccgac ggaatcgcca tggcacgcga
     2701 aagtcgtcta cccgagaagg taatcagctt catacccgag caccacggca ccacgacgat
     2761 ccagtatttt taccacaagg cgctcgaggc acagtcagca cggtcgagcc atgcccatgt
     2821 cacgcgcagc catttccagt acccgggacc aagaccgcgc accaaagaaa ctgcagtcgc
     2881 gatgatagca gacagcgtag aggcagcctc acgcactgcc tctacggctt ctcccgaaga
     2941 gttcgccgca atcatagacc gcattatcca gaacaagatt tccgaagaac agtttcatga
     3001 agcgcccttg accctgggtg acctgtcgac gatacgcaag gcgtttctcg atgtgctggt
     3061 cagcacctac catgagcggc cccagtaccc gacgatgcag gaaacaaagg ccctggaagt
     3121 ttctgtacag agcatgccgg aaaaaaggtc agtcgctagg ggcaaacagc agcgtaagca
     3181 gaaacgctcc gccgcgctgc cacagctcat tgactgacat gactgcaatc ttcaggcgca
     3241 atatacagat tctacgcgac ggctatcggc aggcagacca gccgacccgg cgccgccacg
     3301 tcatactatt cgccctgaac ttcctaacgc tctttctcgc gggggcaaat ttcagcgcac
     3361 acagccaggc agcaggccgc aatttcgatg cgctggtttt cgcgctggcg ctctcggtca
     3421 ttttgctcgg ctattcactc gcccgcttta tccaggctcg ttcgtatggc ctgtattcca
     3481 ccctgccctt cttcattccg atgccgctgt tttcgccgtt cggtacactc ggtgttgtga
     3541 cgcgcaccgc gcacaacggc gctggtagcc gcgcactttt tgatgtggcc ttctgggggc
     3601 ctgcgaccag tttccttttg tcggtgccct gcctggtcgc aggcacactg ctgaccgatg
     3661 tcgtggcagg cgtgccacag tttgaaaacc cactgttact cagggggatt gccaaagtcc
     3721 tgaaagaaat tccgcatggc tatgatcttg ccgtgcaccc attgctggca gcaggctggg
     3781 caggactgtt ttttaccgcc attaatcttt ttccgctcgg caatcttgcc ggcggacaga
     3841 tcgcctacac attgtttggc cagcgccagc gggacattgc ctacatcttc atggcaatct
     3901 tgttcgtcat ggcgctgtgg tatcccatgt ggtttgcttt cgttctgatt ttcatctacc
     3961 tcggaatcga acatccggaa ctgcgccaga atcgaaaccc cttcttccag gaaatgccac
     4021 aggctgcgat caggcaacca ctcgatcgtg cgcggcagta ccttgcagcc gcctgtgcac
     4081 tggtgtttgc actttctttt accatgcgtc cattctcatc aacgctcgag cgggtcgagc
     4141 agcggccacc tgcggaactg gtgccgccgg accactacgg aaccccgcct gccccgcagg
     4201 agcaggcaga accagcagaa gaaaactcaa tttaggagct tgaaccggcc aataccggtt
     4261 cagaaacaaa tatggcagac gataaaaaaa ttattttttc gatggtcggc gtcagcaaag
     4321 tctacccgcc gcacaagcag gtactcaaag atatttacct gtcattctat tatggtgcga
     4381 aaatcggcat tattggtctc aacggctcgg gaaaatcttc gctgttgaaa attatcgccg
     4441 gtgtcctgaa accagacaaa ggtgaagttg tcttctcgcc cggctacagc gtgggctacc
     4501 tggaacagga accacagctt gatgaaagca aaacggtccg ccagatagtt gaagaaggcg
     4561 ccggtgaagt tgtagcgctg ctcaaagaat acgaagccgt gagcgaaagt ctcggtaccg
     4621 acctgtctga cgacgacatg gaaaaagcca tcgagcgcca gggcgaactc ggagaactga
     4681 tcgagaaagc aaatggctgg gaactcgaca acatgctcaa gcgggcgatg gatgctctca
     4741 actgccccga agaaactgcc gttatcaaaa atctttcggg tggtgaaaaa cgccgcgtgg
     4801 cgctctgccg cctgctgctg cagaaaccac agattctgct gctcgacgaa ccgaccaacc
     4861 acctcgatgc cgaatcggtg cagtggctcg aacagcacct gaaagaatac gagggcacag
     4921 tgatctcgat cacgcacgac cgctacttcc tcgacaatat cgccggctgg atactcgaac
     4981 tcgaccgcgg cgagggtatt ccctggaagg gcaactactc gtcgtggctc gaacagaaat
     5041 cgaaccgtct cgccaatgaa gaaaaagccg agagcaagcg ccgcaagacc cttgagcggg
     5101 agctcgagtg ggtacgcatg ggcgccaaag gacgccaggc aaaatcaaag gcccgcctgg
     5161 gcgcatacga aaaacttctg aatgaagacg ccaaagagaa agagagcaaa ctcgaaatct
     5221 acattcccga tggcccacgc ctcggcgaca aggtgatcat cgcggaaaat gtcagcaaga
     5281 gttttggcga caagctgctt tacgagaacc tgaactttga actgccgccg aacggtatcg
     5341 tcggcatcat cggccccaac ggcgcgggta agacgacgct ctttcgcctc atcatggggc
     5401 tcgaaaagcc cgacgtcggc aactttacta tcggcgagac ggtcaaaatt tcgtacgtcg
     5461 accagcagca ttcggcgatt gaccccaaca agacggtgtt cgaggtcatt tcagggggag
     5521 ctcctgaagt gaaggtgggc aaactctcag tgaacgcgcg cgcctacatc gcgcgcttca
     5581 atttttcggg cgctgaccag gaaaagaaat gcggcgtgct ttcgggcggc gagcgcaacc
     5641 gcctgcacat ggcgctggcg ctcaaagaag gcggtaacgt attgctgctc gacgaaccta
     5701 ccaacgatat tgacgtcaat accgtacgcg cgcttgaaga aggccttgaa gctttcgccg
     5761 gttgcgccgt catcatttca cacgaccgct ggtttctcga ccgcgtcgcg acccacatac
     5821 tggcgtttga aggcgatgcg caggtacgct tttttgaagg cggcttttct gaatatgaag
     5881 ccgatcgcaa gaaacgtatg gggggcgagc ccaaacgttt ccggtacaag aaactggtga
     5941 agtaacgcct caggcctctt cgggggttgc ttcgctggct ttcagggcct ctggctcgct
     6001 ggcgaagaat cggccggcaa agatgcggct tagaatctcc tgctggacgg gttcatcttc
     6061 aaatgcaaga atctccataa cagctacccg gacataggat atttgtcaga ctagccgtcc
     6121 acaagcgaat acaagtgtaa cgcctgcgca ggcggagcgc cgacttgaaa aaagcttacg
     6181 ctcccaatag gcagaaggaa acttgccgca tgtttggaca tatcgcatcg ctggcatttt
     6241 tctttgcgct gactctgccg ctggcgtctg ccaagaacct gatacgcgaa gatgtctacg
     6301 tcaggccggg ctttgaccag gcctggctga agagctggcc cgagcgcacg aaatccgccg
     6361 gcgactggcg ccatgtacct tcggcagccg ccgacaatcg ccccgtcaaa attgcgacgc
     6421 tcggcgcacc ttttcccgaa cccggtattt accgctggcc agtcgagaaa atccacgact
     6481 acaccgctgt cactcggttt tttcttacac cggaagaaaa gaaatcgcta catgcatggg
     6541 gtctgcgact cgggagcatc gctgacaact ggcagatttt cctgaacggc acactcatcg
     6601 ctcaggcatg gcacgccgat gccgcgaatt cgaagatcac ggtgcaccgc gcggtgcgcg
     6661 acgcagtctt cgaattgccg gcggctctgc tggtacccgg cgaaaacctg ctggcgtttc
     6721 gtctcgcggg cgacgcaggc agtgcagaaa tcggcttctt tttagggcag ccgtatgaag
     6781 tggatttttt gcggaacctg ttacagaaac gcagcgagac agttgtcctt attctgatct
     6841 gcctgtacct cgcagtggga ttctaccacc tgctgctcta ttcgcgccgg cagcaggaaa
     6901 gctacaacct gtactttgcg ttattctgta ttggcctgtt catctactta ttcacgcgaa
     6961 caagtgtggt tttcgacatc tttgccgatt cgacgcttat acaaaaagtg gaatatattg
     7021 tactcttcaa tgtgctcacc ccgtttctcg tgtttatcga taaactgttc tatacccgcg
     7081 tcacgctgtt tcccaaaatt tacggagttt tcgcgcttct gctttcgatc gccgtggtgc
     7141 cgacaacctc ccattttaat accactattc tgcgcacgtg gcagctgaca gcgttgctgg
     7201 gtattcttta ctttcttgcc ttccagcttg tacctgtggt cgtgcgtgag gtgcgcagtc
     7261 gctatgccgc ttcacagcgc agcccggcca ttctgcgctt cttctcgtcg atgcagggaa
     7321 cgctgctgcg ctctcctgcc ggcaacctgc tcatcggcat tctgaccatc gtttcaaccg
     7381 cggtgtttga tatcctggat tcgattttct tttctaccgg catcgccttt accaagtacg
     7441 gttttgccat gtttgtactc ggtatcgcgg tgatgctcgc gaaccgcttt ctgcaggtac
     7501 acaatgaagt cgaagagctc aatgcaacgc tcgagaaaaa agtcgaagag cgcacccgcg
     7561 agctacagga aagccttgtg cgggtacagg atctcaagaa ccagcaggat gctgactact
     7621 ttctgactgc cctgctgcta aaaccgctgg cggccaacac cgcgcacagc cagagtattc
     7681 atgtcgaata cctgattaag cagaagaaga ccttcgagtt ccgcaaatgg aaatcggaaa
     7741 tcggcggcga tatcaacatg gcgtcgtcga tctcgctcaa agacaaaccg tacgtcgtgt
     7801 tcgtcaacgc cgacgcgatg ggtaaatcga tgcagggtgc gggcggcgca cttgtactgg
     7861 gtgcagtctt ccagtcgacc attgagcgta tgcgctggac aagagagtta tcagacgttt
     7921 ttcccgaaat ctggctgcac acaacctttc tcgaattgca ccgcattttt gaaagcttcg
     7981 aggggtctat gctgatctcg ctggtgatcg gtctgatcga tgaaagcaac gggctcatgt
     8041 attacatcaa cgcagaacat cccagtattg cgctgctccg taacggcgaa gcaagctttc
     8101 tcgacgcgca tattctgcgc aaactcggca ctccgggcat acacggttcg ctgaagatcc
     8161 actgcgtaca gcttgaaccg ggcgatgtgc tcgttatggg gtcggacggt cgcgacgatg
     8221 taatactggg acacaacccc gacgggtcga acatcatgaa cgagaatgag cagctctttc
     8281 tcgaacacgt gaccgccgcc cgcggtgacc tgcaggagat ttttaaccgc gtacaacaga
     8341 cgggagagat catggatgat ctctcactgc tgaaaattga gtacaatccg gcagagaaga
     8401 atgccgcacc ggctgacctg cagcagttag cagaactgcg cgaacaggca gcgcagctta
     8461 ttgcgcgcga taaacacgcc gaggccgcag aggtctatat gcgcgtgtac gaactcgacc
     8521 cgcaggatat cgaaacctgc tatctgatcg cgcagtgcct gcgccgcgag aaaaagatca
     8581 aacaggcgct gcagtgggct gaacgctgcg tactgcgcga cggcgagaac ctgaaatacc
     8641 tgctacaggc agcggagctc aacatcatga tgaaacgttt taccaaagcc ggaagtttta
     8701 tccgccgggt aaaagaactg gagcccgaac accagcgcgc agcaaaacta gaaacgatga
     8761 ttcaccagta agaggagaca ggcaatgaa
//
LOCUS       DAOQJF010000113         8744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4298_length_8744_cov_5.108413, whole genome shotgun sequence.
ACCESSION   DAOQJF010000113 DAOQJF010000000
VERSION     DAOQJF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_8744_cov_5.108413"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PLF85_07510"
     CDS             complement(<1..1772)
                     /locus_tag="PLF85_07510"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNE19549.1"
                     /translation="MRRSLRFISQSWIPVTAMVTVVAMGIATHNYVYAQAERFVGKRI
                     RKVDFKGNVNVSRDTIYNDIIQMKIGQTLTLPLINGDIKALFGEGSFAYARIEGEDFE
                     DGVAVTFHLQERPRVKDITFLGLDELSATDVQQAIPLKEDDIYTERKIADSIQRILGK
                     YREKGLFNASIRVRKSEVDPAKNTINITFVVDEGEQIKIAKINLLGVTKADPEDVLAV
                     LELEEDGFIADGTFKNEVFEKDKNSILDFYKNQGFLDVELEDARYDYRWKNPKTQEER
                     VIVITYKIREGDQYFYNGYDLTWDERFLNPTTKKQLFSRDKVEEFFEYTNSDIGSVLD
                     NGKFSRDRGIINYLYSQEGYIYARVQPERTIIPLTKEAIAEKRRAPQQVESEKEGKDY
                     YNLARLERILERSPELQGRKFVHTRFVVSEGDKGFIENIIIKGNKKTLDKVIRRELII
                     HEGELFNSELVQRSRERVHNLQFFKEVNVDARPGSTEGKMNLVIDVEEQPTGTISLGG
                     GYGTNTGFSIFTEVAENNLNGTGQRISGRVEFGPLRTALEASWTEPWLFDTPWSLTLT
                     GFYVRRRVLSGSINIATNDESATY"
     gene            complement(1856..2623)
                     /locus_tag="PLF85_07515"
     CDS             complement(1856..2623)
                     /locus_tag="PLF85_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE19550.1"
                     /translation="MLAFDFYPGNAGRTVVMLHGLFGSGRNFASLAESLKTEAQVFAC
                     DARNHGRSPHTPTHTLDDLVLDLAEFLREQKIDQPVLLGHSMGGLTAMAYARKMPETV
                     RGLIVLDIAPRSYRPGHSGEIAAQKLDVSACATRREIDEQMAAYVPDPVVRQFLQMNL
                     ARDAQGKFIWSNNVAAIEASPERTQFPGFEGVLYGGPVLAIRGLDSDFVRDKDVELMR
                     QAFPRLEMHDLPGAQHWLHHSHREQVTGLILPFLRKI"
     gene            2698..3951
                     /locus_tag="PLF85_07520"
     CDS             2698..3951
                     /locus_tag="PLF85_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19551.1"
                     /translation="MSDPTIRFSANKFWMNWQSLGRAHELDNLMHAGFKKWPAAFSTD
                     YMKVSELAPLEKWASELRQLDAQTLIVIGIGGSSLGGIALKALIDPHSDKLVFWEGPH
                     PSKMQKIAQLAENRQTAVLVVSKSGTTLETRANLSLFRQFFPGVPEYFVTSEPAAIKD
                     LGANAKNTFLIPQNLGGRFSIVSPVGMMPAMFVDYNVKAFLSAFDEANAEWEISIPVA
                     ENVAKQTAIHFYNLFAARFTGVAFWVYAADLVDWGRWLVQLWAESLGKKPHIAALPMI
                     VQGPEDQHSLLQYFMEASNRHLHCFVHTESYGSRDSLITPEVRGDFADKSMSQILNAQ
                     MKSIESALSAKERPVAEYLLSDLSEAEMARWFCFWMHVVSYLGYLWEINPFDQPGVEA
                     GKIICKKLLRGEAVEEAVQPVFDVG"
     gene            3948..4877
                     /locus_tag="PLF85_07525"
     CDS             3948..4877
                     /locus_tag="PLF85_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNE19552.1"
                     /translation="MIAETLILGGIAAGLLMTVRYLRHHPRNFHRYFAGFKPALATHR
                     GHGKHAAVPENTLIAFSKSEKLGFRSHELDVRKTKDGEIMLLHGPRLEQTTSGSGRME
                     DISSFEISNLDAAHYLLSEHPQVKHRFEKVPTLAEVLAAIHPQALINVEIKRDRWDFS
                     YGLEEETLRVVLASPARERVFFSGFHFLTVWRLKRMRCPLPVGLLVEPGLFTDLKNWL
                     YRFILLPDNVHLHRSRDSAGYLRRLKRRGFGLAFWTVNDLAQAQRLFERGADVVITDR
                     MEFIEHFAPKPPQQRPSKRAAKKKPSRKAAARR"
     gene            complement(4931..5437)
                     /locus_tag="PLF85_07530"
     CDS             complement(4931..5437)
                     /locus_tag="PLF85_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19553.1"
                     /translation="MIQFWRLGLKRAPVMWSLAGVLYLLAYVEMAVFVRYEKFLSANN
                     TLLLYAFALLLIGFAFGILMVQAILCRALRYEIGNFETLGFTRLRIVGYYGMQNLFVA
                     LLALVPAVSTDLILSSFFSQKNMPIAAFERAIAAQFLLGIFLMLSFMSFLLVYSRLSP
                     ALLLKEKL"
     gene            complement(5434..7932)
                     /gene="gyrA"
                     /locus_tag="PLF85_07535"
     CDS             complement(5434..7932)
                     /gene="gyrA"
                     /locus_tag="PLF85_07535"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNE19554.1"
                     /translation="MAKGKGKGSGGDEAKLPTTVAKEIIPVEIEDQMKKSYLAYAMSV
                     IVGRALPDVRDGLKPVHRRILHAMNERAWRQDRAYVKSAKIVGEVIGNYHPHGDSAVY
                     DTMVRMAQEFSMRELLIDGQGNFGSVDGDPAAAYRYTEARLTSMAEELLKDIDKETVD
                     FADNFDGTRREPKVLPAAWPNLLVNGSSGIAVGMATKIPPHNLKEVISAIELLIENPD
                     ASIKDLMKKIPAPDFPTGGIIIGKEGLKNAYTTGRGSIKIRAVANIEESSRGREAIIV
                     TQIPYEVRKNDLLSRVADLVNDKKIEGISEIRDESDRTGMRIVFELKKDAQANIVLNQ
                     LYQHTALQINYGIIFLALVNNEPKLLNLKQMLQHYIAHRREVVERRTRYDLKKAEERA
                     HILEGLKIALDFIDEVIKIIRKSETVDIARSSLMKRFKLSEIQANAILEMRLQKLTSL
                     ESKKILEELNEIKKLIGELKAILKSEGKMYGIIKSELGVIAQKHGVDRHSEVSLVDAE
                     SLKMDTEDLIQNENEVITISESGYIRRMPLDTFKRQHRGGKGVISGGKKEDALKHVHF
                     CQSLDYLLFFSTRGKVFYMKAYEVPEASKDARGKHIKGFLSLGADEVVTVIRSVTEFS
                     EKKYLLMLTQQGIIKKLELSQIANAKKGGVMAMQFKASEKDYLVDVAIVDDKDDIFMA
                     TAEGAGLRTNLGKMRAQGRAASGIIGMTLEGQDHMAGLDVVKGNKSYLLVISESGYGK
                     RVKYDEFGSKGRGGKGMAFFKATEKTGKAVAIRTVTDNAEVIVTTLSGMVLRSEVKQI
                     TVQGRTASGVKVVDISSGDKVADMTVYNPENDIK"
     gene            complement(7993..>8744)
                     /locus_tag="PLF85_07540"
     CDS             complement(7993..>8744)
                     /locus_tag="PLF85_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19555.1"
                     /translation="IAVTIHATADRYVVHAEFGGSVAARVVPARSAELRVLLSRLKLR
                     YGRLRITIIDRSCELLSQVRSFGLECGLVLEAERMTDLVRGREREIRDILVVTNLNGP
                     ALPQLMDNLDFWSRGPGGCRFRHIYGQLTARRAESALARRHWDMVIYRGHGQVIDGRI
                     CWQADNPWPVPHGIGDTYLHLSCLDSPHLLDIAQLPAGRVMTPLGLVSDFQDAGFVRE
                     LITRLQKSGNFMSSARALQRAYSDFAVICNS"
BASE COUNT         1833 a         2541 c         2367 g         2003 t
ORIGIN      
        1 ttataggtcg ccgattcgtc gttggtcgca atgttgatcg aacccgaaag cacgcggcga
       61 cgcacataaa aacccgtcag cgtcagcgac cagggagtat caaataacca cggctcggtc
      121 cacgaagctt cgagcgccgt ccgcaaggga ccgaattcga cccggcccga aatacgctgg
      181 ccggtaccgt tcaggttgtt ctcggccacc tcggtaaaga tcgaaaagcc cgtgttcgta
      241 ccgtaaccgc caccgagcga gatcgtaccc gtcggctgct cttcgacgtc gatcacgagg
      301 ttcatcttgc cctcggtcga accgggccgt gcgtcgacgt ttacttcttt aaagaactgc
      361 aggttgtgca cgcgctcgcg cgaacgctgc acgagttcag agttaaagag ctctccctcg
      421 tgtatgataa gttcgcggcg gataactttg tcgagggtct ttttgttacc cttgatgata
      481 atattttcga taaaaccttt gtcgccttcg gacacgacga aacgcgtatg tacgaacttt
      541 cgcccctgca gttcgggact gcgttcgaga atacgctcga gccgcgcaag gttgtagtag
      601 tctttgcctt ctttctcgct ttctacctgc tgcggcgccc tgcgcttttc agcgatcgct
      661 tctttagtca gcggaataat cgtgcgttca ggctgcaccc gcgcgtagat gtagccttcc
      721 tgcgaataca ggtagttgat gataccgcgg tcgcggctga acttgccgtt gtcgagtacc
      781 gaaccgatat ctgagttggt atactcgaaa aattcttcta ccttatcgcg gctaaagagc
      841 tgcttctttg ttgtcgggtt caggaaacgt tcatcccacg tcaggtcgta accgttgtag
      901 aagtactggt cgccttcgcg gatcttgtag gtaataacga tgacgcgctc ttcctgcgtc
      961 ttcgggtttt tccagcgata gtcgtagcgc gcgtcttcga gttcgacgtc gagaaaaccc
     1021 tggttcttgt agaaatcgag gatcgagttc ttgtctttct caaaaacttc gtttttgaaa
     1081 gtaccgtcgg cgatgaagcc gtcttcttcg agctcaagta ccgccagcac atcttcaggg
     1141 tctgccttgg ttaccccgag caggttaatc ttggcgatct tgatctgttc gccttcgtcg
     1201 accacgaagg tgatgttgat ggtgtttttc gccgggtcga cttcgctctt gcgcacccgg
     1261 atactcgcgt tgaagaggcc cttctcgcgg tatttgccga gtatacgctg tatggagtct
     1321 gcgatcttgc gttcagtata gatgtcgtct tctttcagcg gaatcgcctg ctgcacgtcg
     1381 gtggctgaaa gctcgtcaag ccccagaaag gtgatatcct ttacccgtgg cctttcctgc
     1441 aggtggaaag tgactgccac accgtcttcg aaatcttcac cctcaatgcg tgcgtaggcg
     1501 aacgaaccct caccgaacag cgccttgatg tcgccgttaa tcagtggcag cgtcagggtc
     1561 tgccctatct tcatctggat aatgtcgttg tagatggtgt cgcgcgaaac gttgacgtta
     1621 cccttgaaat ccaccttgcg gatgcgcttg ccgacgaagc gctctgcctg cgcgtagaca
     1681 tagttgtgcg tggcaatacc cattgccacg accgtcacca tcgcggttac aggaatccag
     1741 gattgagaga tgaaacgaag actgcgtcgc aatggattat gcccagggtt ttaagtcgta
     1801 accggtattt cagagcaggc gtcaatcgct atggtgcggt gcattcccga acctgctaaa
     1861 ttttccgcaa aaacggcaga atgagcccgg tgacctgctc gcggtggctg tggtgcagcc
     1921 agtgctgcgc ccctggtaag tcatgcatct caagccgcgg aaacgcctgg cgcatcagct
     1981 cgacatcctt gtcgcgcacg aagtcggagt ccagccccct gattgccagc accggcccgc
     2041 cgtagagtac accctcaaac ccggggaact gcgtgcgctc gggcgaggcc tctatcgctg
     2101 cgacgttgtt actccagatg aatttgccct gcgcatcgcg tgcaaggttc atctgcaaaa
     2161 actgccgcac gaccgggtct ggtacatatg ccgccatctg ctcgtcgatc tcgcggcggg
     2221 tcgcgcacgc actcacatcg agtttctgtg cagcaatctc gccgctatgc cccggccggt
     2281 aactgcgggg agctatatcg agcacgatta gaccccgcac tgtttcaggc atttttcgcg
     2341 catacgccat tgcggttagc ccgcccatcg aatgcccgag cagtaccggt tggtcaattt
     2401 tctgttcgcg cagaaattct gcgagatcga gtacaaggtc atctagcgtg tgcgtcgggg
     2461 tatgcggcga acgcccatgg ttgcgcgcat cgcaggcaaa gacctgtgct tctgttttca
     2521 ggctttcggc taaagatgcg aaattacgcc ctgagccaaa gaggccatgc agcatcacca
     2581 cggtgcgccc ggcgttaccc gggtaaaaat caaatgccag cacgcgcgga taatcctgcg
     2641 aaaggcagca gcgcgtaata ttctttacac gtccggttag cgagcgaaag ttccgccatg
     2701 tcagacccga cgattcgctt ttccgccaat aaattctgga tgaactggca gtcgctcggg
     2761 cgcgcacacg aactcgacaa cctgatgcac gcgggcttca agaaatggcc ggcggctttt
     2821 tcgaccgact atatgaaagt cagcgaactg gccccgctcg agaagtgggc ttcagaactg
     2881 cgccagctcg atgcgcagac gctcatcgtg atcggtatcg gcggctcatc tctgggcggc
     2941 atcgcgctca aggcgctgat tgacccccat tcagacaaac tcgtcttctg ggaaggcccg
     3001 cacccgagca agatgcagaa gattgcgcaa ctcgccgaga accgccagac cgcggtgctt
     3061 gtcgtttcga agtcgggtac aactctcgaa acacgcgcca acctttcgct cttccgccag
     3121 ttttttcccg gtgtgcccga atacttcgtc accagcgaac cggcggcaat taaagacctg
     3181 ggcgccaacg caaagaatac cttcctaata ccccaaaacc tgggcggccg gttcagtatc
     3241 gtttcgccgg tgggaatgat gcctgcaatg ttcgtcgact acaacgtgaa ggcatttctg
     3301 agcgcctttg acgaagccaa cgccgaatgg gagatttcaa ttcccgtcgc ggaaaacgtc
     3361 gccaaacaaa ccgcaatcca cttctacaac ctttttgccg cacgctttac cggcgtcgct
     3421 ttctgggtct acgctgccga ccttgtcgac tggggtcgct ggctggttca gctctgggca
     3481 gaatcgctcg gcaaaaagcc gcacatcgct gcgctgccga tgatcgtgca gggccccgaa
     3541 gaccagcatt cgctgctgca gtatttcatg gaagcgtcta accgtcacct gcactgcttc
     3601 gtgcatacag aaagttatgg ttcgcgcgac agcctcatta cgcctgaagt gcgcggcgat
     3661 ttcgccgaca agtcgatgag ccagatactc aacgcgcaga tgaagagtat cgaatcggcg
     3721 ctgtcggcca aagagcgccc ggtagccgag tacctgctga gcgatctttc agaagctgag
     3781 atggcgcgct ggttctgctt ctggatgcac gttgtctctt acctgggcta cctgtgggag
     3841 atcaacccat tcgaccagcc aggcgtcgag gcaggcaaaa tcatctgcaa gaagctgctg
     3901 cgcggcgaag ccgtcgaaga agccgtacag ccggtctttg acgtcggatg atcgccgaaa
     3961 cactaattct cggcggcatt gccgccgggc tgctgatgac ggtgcgctac ctgcgccatc
     4021 acccgcgcaa ttttcaccgg tattttgccg ggttcaaacc ggctcttgcc acgcaccgcg
     4081 gtcacggtaa acatgcggcg gttcctgaaa atacgctcat cgccttcagt aaatcagaga
     4141 agctcggatt tcgttcgcac gaactcgatg tacgtaaaac aaaagacggc gaaatcatgc
     4201 tgctgcacgg gccgcggctc gaacaaacca cgagcggctc agggcgcatg gaagatatat
     4261 catctttcga aatatcgaac ctggacgctg cccactacct gctcagcgag cacccgcagg
     4321 taaaacaccg gtttgaaaaa gtaccgacac ttgccgaagt gctcgcggct attcacccgc
     4381 aggcgctcat caatgtcgag atcaagcgcg accgctggga cttctcttat ggtcttgaag
     4441 aagaaacgct gcgcgtggtg ctcgcctcac ccgcgcgcga gcgcgtgttt ttctcaggct
     4501 tccattttct gactgtctgg cgcttaaaaa gaatgcgctg cccccttccc gtgggattac
     4561 tcgttgaacc gggccttttc acggatttga aaaactggct ctaccgtttt attcttttgc
     4621 ccgacaatgt acacctgcac cggtcgcgtg attcggccgg ttacctccgg cgcctgaaac
     4681 gccgcggctt cggccttgcc ttctggacag taaacgacct tgcgcaggct caacggctgt
     4741 tcgagcgggg cgctgatgtg gtcattaccg accgcatgga gttcattgag cactttgcac
     4801 caaagccacc gcaacagcga ccttcaaaac gcgcggcaaa gaagaagccg tcacgcaaag
     4861 cagcagcccg gcggtagtcg ccgaagtagc tgcaacgacc gcgtgatttc tgccaacgcg
     4921 gcctggtgca tcagagtttt tctttgagca gcagcgctgg gctcagccgc gaatatacca
     4981 gcaggaagct cataaaactg agcatgagaa aaataccaag taaaaactgc gcagcgattg
     5041 cccgctcgaa agccgcgatg ggcatattct tttgcgagaa aaaactgctc aggatcagat
     5101 cggtagagac tgccggcacc aaagccagca gcgccacgaa caggttctgc atgccgtagt
     5161 agccgactat cctaagccgg gtaaaaccca gcgtttcaaa attgccgatt tcatagcgca
     5221 gcgcccggca gagaatcgcc tgcaccatca gtatgccaaa ggcaaaaccg atcagcagca
     5281 aggcgaatgc ataaagtaaa agcgtattgt ttgccgagag aaacttttca tagcggacaa
     5341 aaaccgccat ctcgacgtat gccagcaggt agagaactcc tgccagcgac cacatcacgg
     5401 gtgcgcgttt gagccccagt cgccagaact gtatcatttg atgtcgtttt cggggttgta
     5461 gactgtcata tcggcaacct tgtcgcccga gctgatatcc acaaccttca cacccgaggc
     5521 agttctgccc tgcacggtga tttgcttcac ctcgctgcgc agcaccatac ctgacagcgt
     5581 tgtgacaatc acttcggcgt tgtcggtcac ggtcctgatg gcgacggcct tgccggtttt
     5641 ttccgttgct ttaaagaagg ccataccttt accaccgcgg cccttgctgc caaactcgtc
     5701 gtatttaacg cgtttgccgt aaccgctttc gctgatgacc aacaggtacg atttgttgcc
     5761 tttcacaacg tcgaggccgg ccatgtggtc ctggccttcg agcgtcatgc cgatgatacc
     5821 cgaagccgcc ctgccctgcg cgcgcatctt accgaggttg gtgcgcaggc cggcgccttc
     5881 ggcggtggcc atgaagatgt cgtctttgtc atcgacaatc gcgacatcga ccaggtagtc
     5941 cttttccgat gccttgaact gcatcgccat gaccccgccc ttcttggcgt tcgcaatctg
     6001 cgacagctcg agcttcttga tgatgccctg ttgcgtcagc atcagcaggt actttttctc
     6061 gctaaactcg gtgaccgagc ggatgaccgt cacgacttcg tcggcgccga ggctcaggaa
     6121 gcccttgatg tgcttaccgc gagcgtcttt cgacgcctcg ggcacttcgt aagccttcat
     6181 gtagaagacc ttgccgcggg ttgagaagaa cagcaggtaa tcgagactct ggcagaaatg
     6241 gacatgcttc agcgcatctt cttttttgcc gccggagatc actcccttgc cgccgcggtg
     6301 ctgccgcttg aaagtatcga gtggcatacg gcgaatatag ccgctttcgg aaatcgtgat
     6361 aacttcattc tcattctgga tcaggtcttc tgtatccatc ttaagcgatt cggcatcgac
     6421 gagcgacact tctgagtggc ggtcaacgcc gtgcttctgc gcaataacgc ccagttcgct
     6481 cttgataatg ccgtacatct taccctcaga cttgaggata gctttcagct cgccgatcag
     6541 cttcttgatc tcgttgagtt cttcaaggat cttcttcgat tcgagggagg tcagtttctg
     6601 caggcgcatc tcgagaatcg cgttcgcctg tatctctgac agtttgaagc gcttcatcag
     6661 ggacgaacgc gcgatatcga ccgtctcgct cttgcgaatg atcttgatga cctcgtcgat
     6721 aaaatcgaga gcgatcttaa gaccttcgag tatgtgcgcg cgttcttctg cttttttcag
     6781 gtcgtagcgc gtgcgccgct cgactacctc gcggcggtgc gcgatgtagt gctgcagcat
     6841 ctgcttgagg ttcagcagtt tgggctcatt gttgacgagc gccaggaaga taataccgta
     6901 attgatctgc agcgccgtat gctggtaaag ctggttcagc acaatattcg cctgcgcgtc
     6961 ttttttcagt tcgaagacga tacgcattcc cgtacggtca gattcgtcgc gtatttctga
     7021 aatcccctcg atctttttgt cgttcacgag gtctgcgacc cgggaaagca ggtcgttctt
     7081 gcgtacttcg taagggatct gcgtgacgat gattgcctcg cgaccacgcg agctttcttc
     7141 gatgtttgct accgcgcgga tcttgatcga accacgaccg gttgtgtagg cattttttaa
     7201 tccttcttta ccgatgataa tgccgccggt cggaaaatcc ggggcaggaa tcttcttcat
     7261 caggtctttg atgctcgcat cgggattttc aatcagcagc tcaattgccg agataacttc
     7321 tttcaggttg tgcgggggaa tctttgtcgc catacccacc gcgatacccg acgaaccatt
     7381 gaccaggagg ttaggccagg cagcgggcaa aaccttgggt tcgcggcgcg tgccgtcgaa
     7441 gttatccgcg aaatctaccg tttctttgtc gatgtctttg agcaattctt ctgccatcga
     7501 ggtaagccgc gcttcggtat aacgataagc agccgccgga tcgccgtcga ctgaaccgaa
     7561 gttaccctgc ccgtcgatga gcagttcgcg catcgaaaac tcctgcgcca tgcgcaccat
     7621 ggtatcgtag acggcagagt cgccgtgcgg gtggtagtta ccgatgactt caccgacaat
     7681 tttcgccgac ttgacgtagg cgcggtcttg ccgccaggca cgctcattca tcgcgtgcag
     7741 aatgcgccgg tgcacgggct tcaaaccatc gcgtacatcg ggcagcgcgc gaccgacaat
     7801 aacactcatc gcgtacgcaa ggtacgactt cttcatctgg tcttcgattt cgaccgggat
     7861 tatttctttt gcgacagtgg ttggcagctt cgcttcatcg ccgccagaac ccttgccttt
     7921 gcctttcgcc atgtgacata atagagggaa ctttcaggcg gtcgaccaaa agaaacatac
     7981 ggaagctttc cgctagctgt tacagatgac cgcaaaatcg ctataagcgc gctgcagggc
     8041 gcgcgcggag ctcatgaaat tcccggactt ctgcagccgg gtaatcagct cgcgcacgaa
     8101 cccggcgtcc tggaagtcgc tgaccaaccc cagaggggtc atcaccctgc ccgcaggcag
     8161 ctgcgcaata tccagcagat ggggtgaatc aaggcacgaa aggtgcaggt aggtatcgcc
     8221 aatgccatgc ggcactggcc aggggttatc ggcctgccag cagatacggc cgtcgatgac
     8281 ttgtccgtgc ccgcggtaga tcaccatgtc ccagtggcgg cgggccagcg cgctctcagc
     8341 gcggcgggcc gtcagttgcc cgtagatatg gcgaaagcgg caccccccgg gcccccgcga
     8401 ccagaagtcg aggttgtcca tcagctgcgg caaggccggc ccgttcaggt tggtaaccac
     8461 caggatatcc ctgatctcgc gttcgcgccc ccgaacgaga tcggtcatgc gctctgcttc
     8521 gagcaccagc ccgcattcca ggccaaagct gcgcacctgc gacagcaact cgcacgaccg
     8581 gtcgatgatc gtgatacgca gcctgccgta gcgcagcttg aggcgcgaga gcaggacgcg
     8641 caactccgcc gagcgcgcgg gtacgacgcg ggcagcgacg gaaccgccga actcggcgtg
     8701 taccacgtaa cggtccgctg tcgcgtggat tgtcaccgcg atct
//
LOCUS       DAOQJF010000114         8694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4344_length_8694_cov_5.214261, whole genome shotgun sequence.
ACCESSION   DAOQJF010000114 DAOQJF010000000
VERSION     DAOQJF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_8694_cov_5.214261"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLF85_07545"
     CDS             complement(<1..500)
                     /locus_tag="PLF85_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19556.1"
                     /translation="MLPEWQKFLRSNRRGYLSLALIAAPAMLLFAQDGTRPDATRRKS
                     FAAGEAFLWHHAENLEQIRQGNTTVIRLERKADNQGTADDLVIDFEHPALFRNPAHQS
                     LVMKQSLERAAVNDLSGKAARFNLPGHQLKLRLPPYLHLSGNAGEGPAGDFSFSCELK
                     PATAGG"
     gene            complement(463..891)
                     /gene="rpiB"
                     /locus_tag="PLF85_07550"
     CDS             complement(463..891)
                     /gene="rpiB"
                     /locus_tag="PLF85_07550"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNE19557.1"
                     /translation="MKIAIAADHGGYELKEVLKQHFAAYNFTDLGTNSNESVDYPDYG
                     AALARRVAAGEFDRGILICGSGIGISIAANKVKGIRAALCHNAYTAEMSRRHNDANVI
                     AMGGRVVDAKTAVEMTDIFLKTEFEGGRHAARVAKISALE"
     gene            complement(888..1244)
                     /locus_tag="PLF85_07555"
     CDS             complement(888..1244)
                     /locus_tag="PLF85_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HNE19558.1"
                     /translation="MDVKLVSLYVMAGFYALAGIAHFANPKFFLRIMPPYIPWHKPIV
                     YLSGVAEIGLGILLLLPEYSSWAAWGIIALLVAVFPANLYHFTSGGAGMRIPKWALAL
                     RLPLQAVLIVWAWWYV"
     gene            1380..2594
                     /locus_tag="PLF85_07560"
     CDS             1380..2594
                     /locus_tag="PLF85_07560"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE19559.1"
                     /translation="MKLASRLELIEPSPTLAITARANELKAEGKDIVGFGAGEPDFDT
                     PEHIKAAAIDALNKGFTKYTAVSGIAELKQAIIAKFARDNQLEFKPNQIIVGTGGKQV
                     LYNFFLSVINDGDEVVCPAPYWVSYKDIVRLAGGTMKLINTTFESGFKVSPAQLEQAL
                     TPRTRVFILNSPSNPTGATYTPEEIRALAAVLEKFPDLLVVTDDIYEKLAYGIEILNI
                     ATASEKLRPRTVVINGLSKAYSMTGWRLGYAGGPKEIIAAMDMIQGQSTSNATSFSQK
                     GAVVALNADQKCVEDMRIVFEKRRDLAFTLLSGLKGLRVFKPQGAFYLFPDIAGLKEA
                     PGWADLAKRTGETDTGKIFSTGLLNEKLVAVVPGSAFGYENGFRISYATSEAQIEKGL
                     GRVREFVEALWK"
     gene            complement(2621..2788)
                     /locus_tag="PLF85_07565"
     CDS             complement(2621..2788)
                     /locus_tag="PLF85_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19560.1"
                     /translation="MNIDPVADLAASMADMSQMLVKLNDAKLALDEKMLGVASEEAAQ
                     SLAAGSVNVTA"
     gene            2936..3463
                     /locus_tag="PLF85_07570"
     CDS             2936..3463
                     /locus_tag="PLF85_07570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE19561.1"
                     /translation="MNLENLAKEPESAAWIDFQDCDPFGHLNNARYLNYFMQARTQQL
                     KDYYGFDLYAHTATTGNGWVVAQTHLAYLVPAKFNENVRIRTRLLHYDNFRLVPEAVM
                     LSADGERVHALGWIEFIYVNAAKGRPVKYDEELQSFFAAINTAQPCAAADFSVRVKEL
                     QKLNRSPARAVAVPA"
     gene            complement(3480..3728)
                     /locus_tag="PLF85_07575"
     CDS             complement(3480..3728)
                     /locus_tag="PLF85_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNE19562.1"
                     /translation="MIISVRVKPRSKKIGFEWDADGTLVLKIHEPPVDGKANKAVIEQ
                     IAELYGLAKSAVRIVSGETARTKRVEIPDGSAPLNPPG"
     gene            complement(3725..4690)
                     /locus_tag="PLF85_07580"
     CDS             complement(3725..4690)
                     /locus_tag="PLF85_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19563.1"
                     /translation="MITQLVGFGLEDKKLLKKFVRLPWKHYSLGEKYVPQLNAELLGN
                     RLLGVQGLLTKQHPYHKNAIVRHWLAYQNGKIVGRISASVNNVYNKHHNTSIGNFGFF
                     ESIKDDSVSAALFGAAADWLRDQGVKTMRGPGQYGNATHEIQSWAMNSFHDAPTVELT
                     MSKWYYPKMAEKFGMYKAKDYYAYLNPVESVEWDRDNKLLERMQTRSRFRIRKMNMKD
                     IHNEVDKIIEIYNEAWKDNWGFLPLTSDDGQAMAETLKMVADENLLLFVMDGEREIAV
                     IGTLPYARSKLRWCSKRTWYSGTCSAISAASNIRSTASTTMTFEA"
     gene            complement(4729..5247)
                     /locus_tag="PLF85_07585"
     CDS             complement(4729..5247)
                     /locus_tag="PLF85_07585"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNE19564.1"
                     /translation="MTELPYRPNVGVVIFNAEGFVLVGERLDNPGAWQYPQGGVDEGE
                     DFEAAAARELHEETGIPLGSSVSGKTPPGQLIARTADFLYYDFPPTLKIPKLTDRYRG
                     QKQLWFLAFWNHPVTLADLKTHTQEFARVRFMPMAAVTNQIVPFKRDIYAALEREFLP
                     IIADYLSKRSTN"
     gene            5351..7228
                     /locus_tag="PLF85_07590"
     CDS             5351..7228
                     /locus_tag="PLF85_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE19565.1"
                     /translation="MAFNNLNEIFYQANDLYGTKKMFFGKNEKKEFVGTTFGDALKAA
                     ENLGIALMELGVKPGDRVGLLADNRVEWIVADMAVILNGAADVPRGTDVTTEEIKYIM
                     THAECSLCIVENESTLRKLQTVIADTTVKTVIVLDPKFKDGSENILTLKQLVEKGASL
                     RDSKLAELKKRADAVKNDDLYTIIYTSGTTGLPKGVMLSHGNIVYNINEVPKAIGLRP
                     DDSMMSLLPVWHIFERAVDYGAIAKGIPVYYTNIRDVRDDFVKVKPTFMPSAPRLWES
                     LYQGIQQRIEKSEPARKMMFETAYRVTKEYKKAVDYLAGNELMAKPESNQERLQRSAQ
                     AIAMAAALYIPAQLADRVVFSKIREAMGGRFRGSISGGGALPAHVDEFFNVIGIPVYE
                     GYGMTECSPIIAVRTEGRIVQGSVGFVPNGTEVAILNDKGERVRTGEKGVIHVRGPQV
                     MKGYYKNPEATEKTISKDGWLNTGDLGFFSFNNTLSIRGRAKETIVLLGGENVEPVPI
                     ENKLLEDAQINQVIVVGQDKKTLTALIWPNLEKVKDVTGITADPKSDLNAHATLREHF
                     GKVIKKQVSAENGFKGFERVTDFRFLPKPMEVGDEITNLLKMKRNVIADKYAALIESM
                     Y"
     gene            7228..8034
                     /locus_tag="PLF85_07595"
     CDS             7228..8034
                     /locus_tag="PLF85_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19566.1"
                     /translation="MAFDRLKRIFGGSKPPKTAPETAGPAPRAPAAEKARRRRAPRIN
                     RRSDSPYVLRPSLQKEAIEAVRAEIRRSPQRYRTDKFTLLEIAEKILHTQLQLPRPAQ
                     LRAHHVFFMFTRTEGRQRKGVTGACFVWKGAVKGGAPTVTTLNAQEGRRLQVDARTYI
                     IENPPKGRRRRLRTIPENSCGNPLCVNARHVRMVPKNPLSHQGQNHPRSKFTDQVIVR
                     MVRDYNAGMTSREVGDKYGMHWTYVEQIMRKEKRTEATAGLKIRGRFGSR"
     gene            8107..>8694
                     /locus_tag="PLF85_07600"
     CDS             8107..>8694
                     /locus_tag="PLF85_07600"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNE19567.1"
                     /translation="MAIKIGINGFGRIGRNAFRAAWGRTDVEIVAVNDLTDTATLAHL
                     LKYDSVFGVWNHDVKHDKEHIIVDGKKIHATAIAKLEEQKWGSFGCDIVIESTGRFTD
                     ATQARAHIDKGGAKQVIISAPATNEDATFVIGVNEKNFDKAKHTVISNASCTTNCLAP
                     VAKVLVENFGIKKGLMTTIHSYTNDQQILDLPHKDL"
BASE COUNT         1989 a         2367 c         2423 g         1915 t
ORIGIN      
        1 tcaccacccg ccgttgccgg tttaagttca cacgaaaaag aaaaatcccc tgcaggccct
       61 tcgcccgcgt tacccgagag atgcaggtaa ggcggcaacc gcagtttcag ctggtgcccc
      121 ggcaggttga accgtgccgc cttgccggac aagtcattca ctgccgcgcg ctcgaggctc
      181 tgcttcatga cgagcgactg gtgagcgggg ttcctgaaca gagcagggtg ttcaaagtcg
      241 attacgagat cgtctgcggt gccctggttg tcggcttttc gttcgagacg aatgaccgtc
      301 gtattgccct gccgaatctg ctcgagattt tcggcgtggt gccagagaaa cgcttctccc
      361 gcagcaaaag attttctgcg agtggcgtcc gggcgagtgc catcctgggc aaatagcagc
      421 atcgcaggcg ctgcgatgag cgccagcgaa agatagccgc gcctattcga gcgcagaaat
      481 ttttgccact cgggcagcat gccgtccgcc ttcaaactcc gtcttaagaa agatgtctgt
      541 catctcgact gcagtctttg catcgactac acggccgccc atcgcgatca cattggcatc
      601 attatgcctg cgcgacattt cggcggtgta cgcgttgtgg cagagcgctg cgcgtatacc
      661 ttttaccttg ttggcggcaa tcgagatgcc gataccgctg ccgcagatca gtatgccgcg
      721 gtcgaactca cccgccgcaa cgcgtcgggc aagggcagcg ccgtagtcgg ggtaatcgac
      781 cgactcattg ctgttggtgc cgagatcggt aaagttgtag gctgcgaagt gttgcttcaa
      841 cacttctttg agttcatagc cgccgtggtc tgctgctatg gcaatcttca tacgtaccac
      901 catgcccaga cgatcaggac tgcctgcagg ggcaagcgca acgcgagcgc ccatttaggt
      961 atgcgcatgc cggcgccgcc gctcgtgaag tggtagaggt tcgcaggaaa cacggcgacg
     1021 agcagggcga tgatgcccca tgccgcccag gaggaatatt cgggcaatag cagcaggata
     1081 ccgagaccga tttcagccac gccgctcagg tagacaatcg gcttgtgcca cggaatatag
     1141 ggtggcatga tgcgcagaaa gaacttcgga tttgcaaaat gcgcgattcc ggccagcgcg
     1201 taaaaacctg ccatcacgta gagtgagacc agcttgacat ccatgcggca ttgcggcaat
     1261 gagacataat gtagcaagga taatgcggcg tgggcgcagg cggtccagga ccgctggctg
     1321 aatgccgccg gcgcacggaa tgcgtgacgg catgtctcaa aaacccattt gggcgaccta
     1381 tgaagctcgc aagccgcctc gaactgattg aaccatcgcc cacgctggcg atcaccgccc
     1441 gcgcgaacga actcaaagcc gaaggcaaag atattgtcgg ttttggcgcc ggcgagcccg
     1501 acttcgacac ccccgaacac atcaaggctg cggcgatcga tgcgctgaat aagggcttca
     1561 cgaagtacac tgcagtctcg ggcattgccg agctcaagca ggcaatcatc gcgaagtttg
     1621 cccgcgacaa ccagctggaa ttcaagccga accagatcat cgtcggtacc ggcggcaagc
     1681 aggtgctcta caattttttc ctttctgtca tcaatgatgg tgatgaagtc gtctgcccgg
     1741 cgccttactg ggtaagttat aaagatatcg tgcgcctcgc gggcggtaca atgaagctga
     1801 ttaataccac cttcgagagt ggtttcaagg tttcgccggc gcagctggag caggcgctca
     1861 cgcccagaac ccgcgtgttc attctgaact ccccctcgaa ccccaccggt gccacttaca
     1921 cgccggaaga aattcgcgcg ctcgcagcag tgctcgaaaa gtttcccgac ctgctggtgg
     1981 tgaccgacga catctacgaa aaacttgctt acggcatcga aatcctcaat atcgcgactg
     2041 cctcagaaaa actgcgcccg cgcacggttg tgattaacgg cctctcgaag gcttattcga
     2101 tgaccggctg gcgccttggc tacgcgggtg gcccgaaaga aattattgcg gctatggaca
     2161 tgatccaggg ccagtcgacc tcgaacgcaa ccagcttttc acagaagggc gcagtcgtag
     2221 ccctgaacgc cgaccagaag tgcgtcgaag acatgcgcat tgttttcgaa aagcgccgcg
     2281 atctggcctt cacgctgctc tcgggcctca agggcctgcg cgtttttaaa ccgcagggag
     2341 ctttctacct gttcccggat attgccgggt tgaaagaagc tccgggctgg gcagatctcg
     2401 cgaagcgcac aggcgagact gataccggca aaatattctc tactgggctt ttgaacgaga
     2461 agttagtggc tgttgtgccg ggttcggcgt ttggttatga gaacggtttc cgtatcagtt
     2521 acgcaacgag cgaggcgcag atcgagaaag gcctcggccg cgtgcgcgaa ttcgtcgaag
     2581 cgctttggaa gtaactggct tgaggttcct ttgcggaatc tcaagccgtt acatttacag
     2641 agcctgcagc cagtgactgc gcggcttctt cgcttgccac acccagcatc ttttcgtcga
     2701 gcgcgagctt tgcatcgttg agctttacca gcatctggct catgtcggcc atgctggccg
     2761 caaggtctgc aacggggtca atgttcatga tttatcttcg cccgttcgcc tggctgaact
     2821 tgaaggccag ggatttttcg ctgttttggc tgtcgggtcg cgggcagggc aaaaaaactt
     2881 gccatatagt gtatatacac gtataatatg tgcatataca ctatatggag gcattatgaa
     2941 ccttgaaaac ctggccaaag agcccgagtc tgccgcgtgg attgacttcc aggattgtga
     3001 ccctttcggc cacctgaaca atgcgcgcta cctcaactat tttatgcagg cccgcacgca
     3061 gcagctaaaa gattactatg gctttgattt atacgcgcac accgcaacga cgggtaatgg
     3121 ctgggtcgtt gcgcagacac accttgcgta ccttgtgccg gctaaattca atgaaaatgt
     3181 cagaatcagg acacgcctgc tgcattacga caatttcagg ctggtgcccg aggcggtgat
     3241 gctgagcgcc gacggcgagc gggtgcacgc gctgggctgg atcgaattta tctacgttaa
     3301 cgccgcgaag ggcaggccgg ttaaatacga cgaagaacta cagagttttt tcgcggcgat
     3361 caacaccgcg caaccctgtg cagctgcaga cttttcggtt cgtgtgaaag agctacagaa
     3421 gctgaacagg tcgcccgcgc gcgcggtggc cgtgcctgcc tgagctgggt agcggagttt
     3481 cagcccggcg ggttcagggg tgcagaaccg tcgggaatct caaccctctt ggtgcgtgca
     3541 gtctcgccgc taacaatacg caccgctgat ttcgcgagcc cgtagagttc agcgatctgt
     3601 tcaatgacag ccttgtttgc cttgccgtcg acgggcggtt cgtgaatttt gagaactagc
     3661 gtgccgtcgg cgtcccattc gaagccgatt tttttgctgc ggggtttgac gcgtacgctg
     3721 attatcatgc ctcaaaggtc atagtcgtag atgcggtact tcttatattc gatgccgctg
     3781 aaatggccga acaggttcct gagtaccatg ttctcttcga gcaccagcga agcttcgacc
     3841 gcgcgtacgg cagtgtgccg atcacggcaa tttccctttc gccgtccata acgaaaagta
     3901 acaggttttc atctgccacc atcttcagcg tctcagccat tgcctggccg tcatcgctcg
     3961 tcagaggtaa aaatccccag ttgtctttcc atgcttcatt gtagatttca ataatcttat
     4021 ccacttcatt atggatgtct ttcatgttca tcttgcggat acgaaaacgc gaacgcgtct
     4081 gcatgcgctc gagcagtttg ttgtcgcggt cccattcgac gctttcgacc gggttcaggt
     4141 acgcgtagta gtcttttgcc ttgtacatgc cgaacttctc ggccatcttc gggtaatacc
     4201 atttcgacat ggtaagctcc acagtcggtg catcatgaaa gctgttcatt gcccacgact
     4261 gtatctcgtg cgttgcattg ccgtactggc ctggtccgcg cattgttttt actccctgat
     4321 cgcgcaacca gtcggcagcc gcaccgaaga gcgctgcgga gacactgtcg tctttgatcg
     4381 attcgaagaa gccgaagttg ccgatcgagg tattgtggtg cttgttgtag acgttgttga
     4441 ctgaagccga gatgcggccg acaatctttc cgttttggta ggccagccag tgcctgacga
     4501 tggcgttttt atggtatgga tgctgtttcg tcagcaggcc ctgtacgccg agcaggcggt
     4561 tgccgagtaa ttcggcattg agttgtggaa cgtatttttc cccaaggctg taatgcttcc
     4621 atggtaagcg aacaaatttt ttgagaagct ttttatcttc gaggccgaat ccgaccagtt
     4681 gcgttatcat attgcgctat ccgttacaga tagtcagggt taacggtatt aatttgtgct
     4741 tcgtttgctg aggtagtcag caataatggg caaaaattcc cgttcgagtg ctgcatatat
     4801 atctcgctta aatggcacaa tctggtttgt taccgcagcc atcggcataa agcgtacgcg
     4861 cgcgaattcc tgcgtatgtg ttttgaggtc agcaagtgtc accgggtggt tccagaatgc
     4921 cagaaaccag agctgctttt gcccgcggta acggtcagtc agtttgggta tcttgagtgt
     4981 tgggggaaaa tcataataga gaaaatctgc cgtgcgtgca ataagctgcc cggggggagt
     5041 ttttccggat acgctgctcc ccagcggtat gccggtttct tcgtgcagct cgcgcgccgc
     5101 ggccgcctcg aaatcttcac cttcgtcgac acctccctgc gggtactgcc aggcgcccgg
     5161 gttatcgagc cgttcgccga ctaatacgaa gccctcagcg ttgaagatga ccacgccgac
     5221 attcggacgg taaggaagct cagtcatgtg gtatgaagct tgccaattgc gaggctgccc
     5281 gtacagctga aataccgccg ccattgacca acaactcttg accgccgcgc tcgccttacg
     5341 agtgagaccg atggcattta acaatttgaa cgaaattttt taccaggcga acgaccttta
     5401 cggcacgaag aagatgttct tcggtaagaa cgaaaagaaa gaatttgtcg gcaccacttt
     5461 tggcgatgcc ctgaaagcag ctgaaaacct tggcattgcg ctgatggagc tgggggttaa
     5521 gcccggcgac cgcgtggggc tgctggccga taaccgcgtc gaatggattg tcgccgacat
     5581 ggcagtcatt ctcaatggcg ctgccgacgt cccccgtggt accgatgtca caaccgaaga
     5641 aattaagtat atcatgacac acgccgagtg ttcgctctgc atcgtcgaaa acgaatcgac
     5701 gttgcgcaag ctgcagaccg tgatcgccga tacgaccgtc aagacggtga tcgtccttga
     5761 tcctaagttc aaagacggtt cagagaatat cctgacgctg aagcagctgg tcgagaaagg
     5821 tgcttcactg cgcgacagta agctcgccga gctgaaaaag cgcgccgacg cggtcaaaaa
     5881 cgacgatctc tacacgatca tctacacctc gggcacaaca ggcctaccca aaggggtgat
     5941 gctttcgcat ggcaatatcg tctacaatat caacgaagtt cccaaggcga ttggcctgcg
     6001 tcccgacgac agcatgatgt cgcttctgcc tgtctggcac attttcgagc gcgcagtaga
     6061 ttatggtgct attgccaaag gaattccagt ttattatacc aacatccgcg atgtgcgcga
     6121 tgacttcgtg aaagttaaac cgactttcat gccttcggcg ccccgcctgt gggaaagcct
     6181 ctaccagggt atacagcagc gtatcgaaaa gtcagagcct gcgcgcaaga tgatgttcga
     6241 gaccgcctac cgcgtgacca aagaatacaa gaaggcagtc gattatctcg ctggcaacga
     6301 gctgatggcc aagcccgaaa gtaaccagga acgcctgcag cgttcggcgc aggcaatcgc
     6361 gatggctgct gcgctctata ttcctgcgca gctcgctgac cgcgttgtgt tcagcaagat
     6421 acgcgaggca atggggggcc gcttccgtgg cagtatctcg ggcgggggag ctctgcctgc
     6481 tcacgtcgat gaattcttta acgtaatcgg tattcctgtt tacgaaggtt atggcatgac
     6541 cgagtgctct cccattatcg ccgtgcgcac cgagggccgt attgtccagg gcagtgttgg
     6601 ctttgtaccc aatggcaccg aagtggctat tctcaacgac aagggcgagc gtgtgcgtac
     6661 cggcgaaaag ggcgtgatac atgtgcgcgg gccacaggta atgaagggtt actacaagaa
     6721 ccctgaagcc acagaaaaaa cgatcagcaa agatggctgg ctcaataccg gcgaccttgg
     6781 cttcttcagc ttcaataata ccctgtcgat tcgcggccgt gcgaaagaga ccatcgtgtt
     6841 gctcggcggc gaaaacgtcg agccggtgcc gatcgagaac aagctgctcg aagacgcgca
     6901 gatcaaccag gtcatcgttg tcggccagga taagaagaca ctcaccgcgc ttatctggcc
     6961 caacctcgaa aaggtcaaag acgttacggg tatcacggct gacccgaaga gcgatctcaa
     7021 tgcgcacgca acactgcgcg agcactttgg caaagtcatc aaaaaacagg tctctgcaga
     7081 aaacggcttc aagggttttg agcgtgtgac cgacttccgc tttttgccaa agcccatgga
     7141 agtgggcgat gaaattacta acctgctgaa gatgaagcgt aacgtcattg ccgacaaata
     7201 cgctgcgctg attgaaagca tgtactgatg gcctttgaca gactgaaacg tatcttcgga
     7261 ggcagtaagc cgccgaagac tgcgcccgaa actgcagggc cagcgccgcg cgcacctgct
     7321 gcggaaaaag ccaggcgcag gcgcgccccg cggattaacc gtcgttcgga ttcgccttac
     7381 gtgcttcggc cgtcgctgca aaaagaggcg attgaggcgg tgcgtgccga aatacgcagg
     7441 tcaccgcagc gctaccgaac cgataaattc accttgctcg aaattgcaga aaaaatcctg
     7501 catacacagc ttcagctgcc gcggccggcg cagctgcgcg cgcaccatgt cttctttatg
     7561 tttacccgca ccgagggcag gcagcgtaaa ggcgtgaccg gggcctgttt cgtgtggaaa
     7621 ggcgcggtaa aagggggagc ccccactgtg acgacgctga acgcacagga agggcggcgc
     7681 ctgcaggttg atgcacgcac gtatattatt gaaaatccac ccaagggccg gcgcaggcgt
     7741 ctgcgtacca tacctgagaa tagctgcggt aacccgctgt gcgtcaacgc ccggcacgtc
     7801 cgcatggtgc cgaaaaaccc gctgagccac cagggccaga accacccccg ctcaaaattc
     7861 acggaccagg tcatcgtccg gatggtccgc gactataacg caggtatgac atcccgcgag
     7921 gtaggcgaca agtacggtat gcactggact tacgtagaac agatcatgcg taaggaaaag
     7981 cgcaccgagg cgacagcagg cctgaagatc cgcggccgtt ttggctcgcg gtaatctatc
     8041 tggggctaat ttggctcgcg gcccttattc ggctcgacag gccgtttttg attttcactc
     8101 gaacctatgg caataaagat tggtatcaac ggttttggcc gtatcggccg taatgcattt
     8161 cgtgctgcct ggggccgcac ggacgtcgaa atagtggcgg tcaacgacct gactgacaca
     8221 gcaaccctcg cgcacctgct caaatatgac agtgtgttcg gcgtgtggaa ccacgatgtg
     8281 aagcacgaca aagagcacat catcgtcgat ggcaagaaga tccatgcaac cgcgatcgca
     8341 aagctcgaag agcagaaatg gggttcgttc ggctgcgata tcgtgatcga atcaaccggc
     8401 cgtttcaccg atgcgaccca ggcacgcgcg cacatcgata agggtggtgc caagcaggtg
     8461 atcatttcag ctcctgcaac taatgaagac gcaaccttcg tgattggcgt gaatgagaag
     8521 aattttgaca aggcgaagca tactgttatt tcgaacgctt cgtgcacaac caactgcctt
     8581 gcacctgtgg caaaagtact cgtggaaaat tttggcatca agaaaggtct gatgaccact
     8641 atccatagct ataccaacga ccagcagatt ctcgacctgc cacacaaaga cctg
//
LOCUS       DAOQJF010000115         8677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4351_length_8677_cov_5.098237, whole genome shotgun sequence.
ACCESSION   DAOQJF010000115 DAOQJF010000000
VERSION     DAOQJF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4351_length_8677_cov_5.098237"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLF85_07605"
     CDS             complement(<1..327)
                     /locus_tag="PLF85_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19568.1"
                     /translation="MHKPGFAPVDFDYLLHSVYTRLVPMSVRWNIGENLYNAFVSTRR
                     KLSSARLGSAFLGDVTVKYLEWGRPQLPKLLLLHGFADSKDGVLPYAHALSRRFHVIA
                     PDLPGFG"
     gene            complement(406..891)
                     /locus_tag="PLF85_07610"
     CDS             complement(406..891)
                     /locus_tag="PLF85_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19569.1"
                     /translation="MRYLFAVCITSLALLAQDNQYKKYRGAWFEISYPANFRVIPSLQ
                     SRGMPDEFDSVFFESPDKQVRFYIFSPQWGGDTPDIALKDTQETYHLDKREDKGGFRK
                     VWYTIRPKKIGRTRSYLETTNDEGNICWVIGIEYTNREAYTKHLPQYEKFKASLRQFA
                     D"
     gene            1039..1833
                     /locus_tag="PLF85_07615"
     CDS             1039..1833
                     /locus_tag="PLF85_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNE19570.1"
                     /translation="MKRYAGLFALTGALLLVSACKTIQQGNMGVERKFGKQNDELLGP
                     GLYFYNPIWTDILVLPLTTENLEVSMALPSKEGLNIEAEMSILYRLKSEKVPSLLVEF
                     GGSEFQQDEQRIVLNVLRSAAADVSANFLAKDMHSGKRSEIETEILKRMREVLEEKGF
                     MFSQVLLKSIKLPPGLYASIEEKLRAEQDAQRMEYVLQQERREAERRKVEAAGVRDSQ
                     KILSQGLNREIIQLRTIEAFRELAKSPNAKVIITDGKAPLMIPDYR"
     gene            1909..2472
                     /gene="lptC"
                     /locus_tag="PLF85_07620"
     CDS             1909..2472
                     /gene="lptC"
                     /locus_tag="PLF85_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNE19571.1"
                     /translation="MRRPRRLFIVFAVLALAAACRKESAFVPELTHSDQLRGADATMR
                     NFTAEAFRNGGILWRLAAVEGYLVQVKDETHLYDLRIEYFESNRRSRDFGKATIVTAK
                     KCILQQGNKFMTISGNVLVEAPQGRKLRTEELFWDESKNSLYSNVAVTVQDGSGNLLT
                     GTRGIVTDRSLSRTVFKGGVGAGSAGF"
     gene            2476..4098
                     /gene="murJ"
                     /locus_tag="PLF85_07625"
     CDS             2476..4098
                     /gene="murJ"
                     /locus_tag="PLF85_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNE19572.1"
                     /translation="MAVAQKKSPQLGEQGAEVLRNSSIFSLFTSLSRVLGLLRDMLKA
                     YAFGTGPLSVAFDIAFRLNNMLRNLVAEGALSQAFVPLYNRYAHESDEKAREAAGVVI
                     VFVAIAMGLITVLVIFSLPALLPLLLAEDHMPPEQLALTINLSQLLFPYLVMMSVASL
                     YMGIQYSHRIFWAASFGPALLNIVILVCFGAYALWVRLAGATFDVQSIYVFSLATLLA
                     AVAQVVFQIYTVHRRGLTPRYSLSLRHGALKALGSMMLPAVFAASMQEIGQLIDVYLA
                     TSLTAKVPEAISALTYSHRLIQLPIGVFGVAVATASLPQLSRIYQADKEGSAFSESLA
                     SSVRLNFFFLVPAVAGLMVFSEPIVRVIFERGAFTARSTDVTALALLCYAPGILAYSL
                     QKLCTISLYAQMNTRTPAVITALTLVLNFLLSLYLMQTMLHGGLAAGSAIAAWCGVMI
                     YTVILLRGRILREPGKLLGGLLRILAINVLFTLLLLGMRKYIFADRALLQLTLCVPVA
                     VAVYLALAHVAHIAEAHVLVDFLNRLRRKVIR"
     gene            4175..4855
                     /locus_tag="PLF85_07630"
     CDS             4175..4855
                     /locus_tag="PLF85_07630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HNE19573.1"
                     /translation="MRFIVLRHQEEEGLGTIADWLKQNGHAYFYVNLFAQQKPPANAL
                     MAYDGLVSMGGPMSVNDTTDLVKTSLALTADFVQAARPVLGVCLGAQAIAKVSGGVVA
                     PSAFELGFDRVQPVAAHPYFASAKLTPADWDVFQWHGENFSLPHGAAHLFSGTAVANQ
                     GFTYKNALGLQFHNELSFDWYTMWYESLRHKFENMDQLKPPSPEMPFAALKTNLFALL
                     DVWMKSLV"
     gene            complement(4876..5505)
                     /locus_tag="PLF85_07635"
     CDS             complement(4876..5505)
                     /locus_tag="PLF85_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNE19574.1"
                     /translation="MGHVGFTAFTRLRQLVSFVRRRNLRTASYAPGGFHTFCADAGVK
                     TFQYRHKNTSAAQYEENLLRLLPLVPQATLIVNDHAEIAARYQLPLHLGQDDNLPADL
                     TVLYGRSTHSLAELEVALSCRPAPSYIALGTMFASATKPEVGTNRSLIADYRKRTQLP
                     LVLIGGISLDNVIELPRADDIFYAVIGDAFRFGATSAGIKKYVESFALV"
     gene            5593..7377
                     /locus_tag="PLF85_07640"
     CDS             5593..7377
                     /locus_tag="PLF85_07640"
                     /inference="COORDINATES: protein motif:HMM:NF011314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNE19575.1"
                     /translation="MDSEINRQLLTATLLRLRHSMAEAEQSYATAIAAVQPENRESLR
                     NLVHYLAMRRRDLHEVQLQLAALGLSSLGRSEGFVLDNLNRVLHHLGAEDGPETSLDW
                     AAAERLLHRNTRALLGEHPRDRHVLIMITSPLPDEADAHWMEQSLRAGMNVLRINCAH
                     GSESDWEKLIAALRDAEKKTGLSCRLLMDIAGPKLRTGPVHGGAEILRLRVERDIFGR
                     VAKPARVTVGAQAADLTFPQEFCAQMRAGDTLQFHDSRGKKRRLRLEAEGDGFVGLCE
                     SGAYILRESRFTLRRGKKEIAQADCGALPFVPGSLLLKPDDYLVLFAGEGEGFPAEPT
                     APGVPPVHARIGCTLGEAVAAIRPGERVLFDDGKIGAVCVSMQPGEVLLRIVRAKESG
                     SRLRGEKGINLPDSKLKIPAITEKDREDLVFVLEHADIVGASFIHSSEDVAELQQLVA
                     RSRRNPGILLKIETQAGFHNLPVILMQALSQNPVGVMIARGDLAVEAGFERLAEVQEE
                     ILWICEAAHVPVVWATQVLENLAQTGQPSRAEITDAAMSVRAECVMLNKGPYIHEATA
                     ALTNILKKMEKHQYKKRSLYRPLQIGLG"
     gene            7388..8209
                     /locus_tag="PLF85_07645"
     CDS             7388..8209
                     /locus_tag="PLF85_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19576.1"
                     /translation="MPDLTRYGGVMRRHGLVCIGFLVISASLSANMRAPVRIERGYSR
                     LLAKPSQLRLSGEKLEFDCPAAHTGKPEPTLFAQQSCAARVTYQLSSAADERVALSFV
                     YSGSEQVEWNLGTGPQNVVSRAVQSPDRLHCTYCPDDMRSLRVAETEADIKKSLRAIT
                     VRYQQALDYDESGHGYFRAGQWTQGFSYELWPLAEWQWDTKITAKLQLTIAARPGFLG
                     IGYRKDEISCAVLVGERQIPVALTIGEPKDGKRTATAEIELQKQPQRLRCSWSAQ"
     gene            8233..>8677
                     /locus_tag="PLF85_07650"
     CDS             8233..>8677
                     /locus_tag="PLF85_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19577.1"
                     /translation="MYYLKFLGGGVIGNIGWCFFAFGMLFFWALHVPASVRELVVFSG
                     RTDTVSGIVSSVDETNMAVNDEVVHLVRFTYLVAGNEFKGISYLRYRPEPTSSVTVEY
                     IVTSPQLARIEGGSYTPFGGVALAAAIFPLLGLIMVIFPLYGNVRR"
BASE COUNT         1787 a         2407 c         2534 g         1949 t
ORIGIN      
        1 gccaaaccct ggcaggtcag gcgcaatgac gtggaagcgg cgcgagagcg cgtgtgcgta
       61 aggcagaact ccgtctttgc tgtcagcgaa gccatggagc agcagcagct tgggtaactg
      121 cgggcgaccc cattcgaggt acttcacggt cacatcgccg agaaatgcgc tcccgagtct
      181 ggcggagctc agcttacggc gcgtgctgac aaaagcgtta tagaggtttt ccccaatatt
      241 ccagcgcacg ctcataggca cgaggcgcgt gtagacgctg tgcagaaggt agtcgaaatc
      301 tacgggagca aaacccggtt tatgcatgcg ccagcatgcg tgtcttattc agaccgccaa
      361 caaaaaatga acacttgttc gcctaaggcg cgttccgtgg gcctgtcagt cggcgaattg
      421 cctcagcgat gctttgaatt tttcgtactg tggcaggtgc ttcgtgtacg cttcgcggtt
      481 cgtgtattcg ataccgatga cccagcaaat attgccttcg tcattcgtcg tttcgaggta
      541 tgaacgcgtg cggccgattt tcttcgggcg tatcgtgtac cataccttac gaaatccccc
      601 tttatcttct ctcttatcca ggtggtaggt ctcttgcgtg tcttttaacg caatgtcagg
      661 cgtgtcgccg ccccattgtg gtgaaaaaat atagaagcgc acctgtttgt cgggtgattc
      721 gaaaaacacg ctgtcaaact cgtcgggcat accgcgcgat tgtaaagagg gtatcacccg
      781 gaaattggcg gggtaagaga tttcaaacca ggccccccgg tattttttgt actggttatc
      841 ctgcgcaagc agggcaaggc tggtgataca gacagcgaac aggtacctca tacggcttgt
      901 taccattctt cagatatccg agaaagtttt ttaggcattc ctcagctatt ctttgccgcc
      961 gtttgtccgc ggttcacgaa tttatctgta gccatttctt cttatgtggt gcatattctg
     1021 agaaaaaacg ggggagttat gaagagatat gcaggtttat tcgcgctcac aggggcgctt
     1081 cttttggtct cggcatgtaa aaccatacag cagggtaaca tgggcgtaga gcggaagttt
     1141 ggcaaacaga acgacgagct gcttggtccg ggactctatt tctataaccc gatctggaca
     1201 gacatcctgg ttctgccact cactactgaa aacctcgagg tgagtatggc gctgcccagt
     1261 aaagaggggc tgaatatcga agccgagatg tcgattctct accggctcaa atcagaaaaa
     1321 gtgccatccc tcctggtgga gtttggcgga tcggagttcc agcaggatga gcagcgcatt
     1381 gtactcaatg tcctgcgctc ggccgcggca gatgtttcgg caaactttct tgcgaaagac
     1441 atgcatagcg gtaagcgctc agagattgag accgaaattc taaaacgcat gcgggaggtt
     1501 ctggaggaga aaggcttcat gttctcgcag gtgctgctga agagcattaa actgccgccc
     1561 gggctttatg cttcaatcga agaaaagctc agggccgaac aagacgcgca gcgcatggaa
     1621 tatgtgctgc agcaggaaag gcgcgaggca gaacgccgta aggtagaagc cgcgggtgtg
     1681 cgcgactcgc agaagattct cagccagggc ctcaacagag aaatcataca gctgcgcact
     1741 atcgaggcgt ttcgtgagct tgccaagtcg cccaatgcga aagtcatcat taccgatggc
     1801 aaggcaccgc tcatgatacc tgattaccgc tgatttgccg ccacgggccg taacagctgc
     1861 ccatatttcg gtttgcccgc ctgagggact ttgcgcctgt tcccgcagat gcgtcggccc
     1921 cggcggttat tcatagtttt tgcggtactt gcgctcgcgg ctgcctgccg caaagaatct
     1981 gcatttgtac ctgaactgac gcacagcgac cagttgcgtg gcgccgatgc gacaatgcgc
     2041 aacttcactg ccgaggcgtt ccgcaacggg ggaattctct ggcgtctcgc ggccgttgaa
     2101 ggctaccttg tgcaggttaa agacgagacg cacctctatg acctgcgcat tgagtacttt
     2161 gagtcaaacc gccggtcgcg tgattttggc aaggcaacaa ttgtcacggc caagaagtgc
     2221 atcctgcagc aggggaataa attcatgacc atttcgggca atgtgctggt cgaagccccc
     2281 caggggcgaa agctgagaac cgaggagctt ttctgggatg agagcaagaa ctccctctat
     2341 tcaaatgttg ccgtcaccgt gcaggatggt tcgggcaatt tgctgaccgg cacgcgcggc
     2401 attgtcaccg atcgctcgct cagccgcacg gtattcaaag gcggcgtcgg tgcaggttct
     2461 gccggtttct gagttatggc cgtagcacaa aaaaaatccc cacagctggg ggagcagggc
     2521 gctgaggtcc tgcgcaattc ttcgatcttc tctctgttta cttctctctc gcgcgtgctg
     2581 gggcttctgc gcgatatgct gaaggcctat gcttttggta cggggcccct cagcgtcgcc
     2641 tttgatattg cctttcgcct gaacaacatg ctgcgcaacc tggtcgccga aggcgcgctt
     2701 tcgcaggcat ttgtaccgct ctataaccgc tacgcacacg agagcgacga aaaggcgcgc
     2761 gaagcggcgg gcgtcgtgat cgtgttcgtt gcgatcgcca tgggattgat cacggtgctg
     2821 gttattttct ccctgccggc gctgttgcca ctgctgctgg cagaagacca tatgccgccc
     2881 gaacaactgg ctctcacgat taacttaagc cagttgctgt ttccttacct tgtcatgatg
     2941 agcgtggcat cgctctacat gggtatacag tactcgcacc gcattttctg ggcagcgagt
     3001 tttggccctg cgctactcaa catcgttatc ctggtctgtt tcggggctta tgcactgtgg
     3061 gtgcgcctgg cgggcgcaac gtttgacgtg cagtcgatct acgtgttttc cctggcgacg
     3121 cttttggctg cggtcgcgca ggttgtcttc cagatctaca cagtgcatcg ccgtggtctt
     3181 accccgcgct acagcctctc gctgcggcat ggtgccctca aggcgctggg ctcgatgatg
     3241 ctgcctgcag ttttcgccgc ctcgatgcag gaaatcggcc agctgatcga tgtttacctc
     3301 gcgacctcgc tcactgccaa agtgcctgag gcgatttcag cgctgaccta ctcacaccgg
     3361 ttgatacagc tgccgatcgg tgtgtttggt gttgctgtcg cgaccgccag cctgccgcaa
     3421 ctttcgcgca tctaccaggc cgacaaagag ggtagtgcgt tctctgaaag tcttgcttca
     3481 tcggtacggc taaatttctt tttcctcgtg ccggcggttg caggactcat ggtgttcagt
     3541 gaacccattg tccgcgtgat tttcgagcgc ggtgctttca ctgcgcgctc gaccgacgtc
     3601 accgcgctcg ctctgctctg ttatgctccc ggtatcctgg cgtactcgct gcagaagttg
     3661 tgtactatct cgctctatgc gcagatgaat acgcggacgc ccgcggtcat caccgccctg
     3721 accctggtcc tgaacttctt actcagcctc tacctgatgc agaccatgct gcacggcggg
     3781 cttgccgcag gttcggcgat cgctgcctgg tgcggagtaa tgatctatac ggtcatcttg
     3841 ctgcgcggcc ggatcttgcg tgagccgggt aagctactgg gcggcctgct gcgcattctt
     3901 gctatcaatg tgctctttac cctgctgctg ctgggtatgc gcaagtatat cttcgccgac
     3961 cgggcgctgc tgcagctgac cctctgtgta ccggtggcgg tcgcggttta tctcgcgctt
     4021 gctcacgtgg cccacatcgc cgaagcgcac gtgctggtgg acttcctgaa ccggctgcgc
     4081 cgaaaggtta ttcgttaggc agagaataaa ttccctgcct aacgaattga aggatattcc
     4141 ttttcgccgc agggcgccct gacaaaaatt ccgcatgcgc ttcattgtgt tacggcacca
     4201 ggaagaagaa ggtctgggca ccatcgccga ttggctaaaa cagaatgggc atgcgtattt
     4261 ttatgtgaac ctcttcgcgc agcagaaacc accggcgaat gccctcatgg cgtatgacgg
     4321 cctcgtttca atggggggcc ctatgtcggt taatgatacc accgatctgg taaaaacttc
     4381 gctcgcgctc actgccgact tcgtgcaggc agcccgcccg gtgctcgggg tctgcctcgg
     4441 cgcgcaggca atcgcgaagg tttccggtgg agtcgttgcc ccgtctgcct ttgaactggg
     4501 ttttgaccgc gtgcaacccg ttgctgcaca cccgtatttc gcctcggcta aattaacccc
     4561 agccgattgg gatgtcttcc agtggcatgg cgaaaacttt tcgctgccgc atggcgcagc
     4621 ccacctgttc agtggaacag cagttgccaa ccagggcttc acttacaaaa atgcgctggg
     4681 attgcagttt cacaacgagc tttcattcga ctggtatacc atgtggtacg agagcctgcg
     4741 gcacaagttc gagaacatgg accagctcaa gccaccttcg cccgaaatgc ccttcgccgc
     4801 actgaagaca aacctgtttg cgctgctgga tgtttggatg aaatcgctgg tctaggggta
     4861 ggtttaaacc tacccctaga ccagcgcaaa agattcaacg tacttcttaa ttcccgcgct
     4921 ggtcgcgccg aaacgaaagg catcgccgat caccgcataa aagatgtcat cagcccgggg
     4981 taattcaatc acattgtcga gcgagatacc gccgatcagc acgagcggca gctgtgtgcg
     5041 cttgcggtag tcagcgatga gcgagcggtt ggtaccgact tcgggtttgg tggcgctggc
     5101 gaacatggtg cccagcgcaa tgtaggacgg ggcgggccgg cagctgagtg ccacttcgag
     5161 ctcggccagg ctgtgggtcg acctgccgta gagtacagtc agatcagccg gcaggttatc
     5221 gtcctggccc aggtgtagcg gcagctggta tcgcgcggca atttctgcgt ggtcgttgac
     5281 aatgagcgtc gcctgtggta cgagtggcaa caggcgcagc aggttctctt catactgcgc
     5341 cgcgctggtg ttcttgtggc ggtactggaa agtttttact cccgcatcgg cgcagaacgt
     5401 atgaaatcca ccaggggcat acgatgccgt tcgcaggttt cgccgtcgca caaaagatac
     5461 aagttgccgc aaccgggtga aagcggtgaa accgacatga cccatcggac agggatcgcc
     5521 gatttgccgc aaagtctttt tctttacggc gcgtgcccac agcgctgacg cagcgtaccg
     5581 tcccgggtta gtatggattc ggaaattaac aggcaattgc tcacagcgac actgctgcga
     5641 ctgcgccaca gcatggcaga agccgagcag tcatacgcta cggctattgc ggcggtacag
     5701 cccgagaatc gggagagcct gcgcaacctc gtgcattacc tggcgatgcg ccggcgtgac
     5761 ctgcacgagg tgcagctgca gctggcagcc ctcggcctgt cgtcgctcgg ccgcagcgaa
     5821 ggttttgttc tcgataatct caaccgggtg ctgcaccatc tcggtgctga agacggccct
     5881 gaaacttcgc tcgattgggc cgcggcggag cgactactgc accggaatac gcgcgcactt
     5941 ttgggggagc atccccgtga ccgtcacgta ctcatcatga ttacttcacc cctgcctgac
     6001 gaagccgacg cccactggat ggagcagagc ctgcgggccg ggatgaacgt gctgcgcatc
     6061 aattgcgcgc atggcagcga aagtgactgg gagaaactga tagcagcgct gcgcgatgca
     6121 gagaagaaga cggggctctc gtgccggttg ctgatggata tcgccgggcc aaaactcaga
     6181 accgggcctg tacacggtgg cgccgagatt ctgcgcctgc gcgtcgaacg cgatatcttt
     6241 ggtcgcgttg ccaaaccggc gcgagtgacc gtgggcgcgc aggccgcgga tctgaccttc
     6301 ccgcaggaat tctgtgcgca gatgcgtgca ggcgacaccc tgcaattcca tgatagccgc
     6361 ggcaagaagc gccggctgcg tctcgaagcc gaaggcgacg ggtttgtcgg cctgtgcgaa
     6421 agcggagcgt atatactgcg cgaatcgcgc ttcacgctga ggcgtggaaa aaaagaaatc
     6481 gcgcaggcag actgcggcgc cctgccattc gtaccgggtt cgttactcct gaagcctgac
     6541 gattacctgg tactcttcgc aggtgaaggc gaaggatttc ccgctgagcc gaccgcgccc
     6601 ggtgttccgc ctgtgcacgc gcgcatcggg tgtaccctcg gcgaggccgt cgcggcgatc
     6661 aggcccggcg agcgcgtgct cttcgacgac ggcaagatcg gcgcggtctg cgtgagtatg
     6721 cagccgggtg aggttttgct gcgcattgtg cgcgcgaaag aatcgggcag ccgcctgcgg
     6781 ggggaaaagg gcatcaatct gcccgacagc aaactcaaaa ttcctgcgat tacggaaaaa
     6841 gaccgcgaag acctggtttt tgtcctcgag catgcagata ttgtcggtgc gtcgtttatc
     6901 cattcgagtg aagatgtcgc ggaactgcag cagctggtcg cccggtcgcg gcgtaatccc
     6961 ggcattctgc taaaaattga aacgcaggcc gggttccaca acctgcccgt gattttgatg
     7021 caggcattgt cacagaaccc cgtcggcgtc atgattgcgc ggggcgacct ggcggtcgaa
     7081 gctgggtttg agcgactggc agaagtacag gaagagatac tctggatctg cgaggcagcg
     7141 catgtgccgg ttgtctgggc gacacaggta ctcgagaacc tggcgcagac gggacaaccg
     7201 tcacgcgccg agatcaccga cgcagcgatg tcggtacgcg ccgagtgcgt catgctcaac
     7261 aaggggccct acatacacga ggcaacggcc gccctaacca atattctgaa gaaaatggaa
     7321 aaacaccagt acaagaaacg cagtctttac cgcccgctgc aaataggcct cggctaattt
     7381 cacttgcatg cctgatctca cccgctatgg cggtgtcatg cgacgtcatg gactggtgtg
     7441 cattggcttc ctggttattt cagcatcgct gtcggctaac atgcgtgccc cggtgcgtat
     7501 tgagcgcggg tacagccgcc tgctggcaaa accctcacaa ctgcggctca gtggtgaaaa
     7561 actcgaattt gactgtccgg cggcgcacac aggcaaacct gaaccgacac tctttgcgca
     7621 gcaatcatgc gcggcgcgcg tgacttacca gttatcgtct gccgctgacg aaagagtggc
     7681 gctgtcattc gtctattcag gcagcgagca ggtcgaatgg aatttaggca ccgggcccca
     7741 gaatgttgtt tcgcgcgcag tacagtcccc tgacagactt cactgcacct actgtcctga
     7801 cgacatgcgt tcattgcgcg tagccgagac tgaagcggat ataaaaaaat ccctgcgcgc
     7861 gatcacggtg cgctaccagc aggccctgga ctacgacgaa agtgggcatg gctatttcag
     7921 ggcggggcaa tggacgcagg gattcagcta cgagttgtgg ccgcttgctg aatggcaatg
     7981 ggatactaaa atcacggcca aactacagct cacgattgcc gcacgaccgg gatttctcgg
     8041 tattggctac aggaaagatg aaatctcctg cgcagtactc gtcggcgaac gccagatacc
     8101 tgtggcgctt actatcggtg aacccaagga cggcaaaagg acggcgacag ctgaaataga
     8161 gctgcagaag caaccgcagc gcctgcgctg tagctggagc gcacagtgac tgcagcccgc
     8221 aagataccac cagtgtatta cctgaaattt ctcggcggtg gcgttatcgg caacattggt
     8281 tggtgttttt ttgcctttgg aatgctgttc ttctgggctc ttcatgtgcc ggcttccgtt
     8341 cgtgagctgg tcgtcttctc cgggcgcact gacacggtca gtggcatcgt ctcttcggtc
     8401 gatgaaacga atatggcggt gaacgacgag gtggttcatc ttgtccgctt tacctatctg
     8461 gttgcgggta atgagttcaa aggcataagc tacctgcgct accggcccga gcccacaagc
     8521 tcggtgacgg tcgaatatat agttaccagc ccgcaactgg cccgaattga aggggggagc
     8581 tatacacctt tcggcggtgt ggctctggcg gcagctattt tcccgctgct cggactgatc
     8641 atggtgatct ttcccctata cggcaatgtt cgcaggc
//
LOCUS       DAOQJF010000116         8661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4361_length_8661_cov_5.319312, whole genome shotgun sequence.
ACCESSION   DAOQJF010000116 DAOQJF010000000
VERSION     DAOQJF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4361_length_8661_cov_5.319312"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..638)
                     /locus_tag="PLF85_07655"
     CDS             complement(93..638)
                     /locus_tag="PLF85_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19578.1"
                     /translation="MLKATAMMKRIPFILLFWLAACQKPPEFVLQGYFFVGAADEAAA
                     QRENPVLVATAASVIESIIAEDREKLLSFVNTKEGAIIDAKAFVSYEQVRSVLYDKSA
                     ILSRVLWDDKYWKETAPGDNVRSYRTVFSRAGEIKLGLFWYSPTECEVRIDFKGRPSM
                     GLMGNLIFRKRGDRWYLMNFF"
     gene            complement(644..2074)
                     /locus_tag="PLF85_07660"
     CDS             complement(644..2074)
                     /locus_tag="PLF85_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19579.1"
                     /translation="MAKDSADAENFSRLLKAILPGELESLQAQDWQSTPLPGEAVYAR
                     FEKPAGITGLGGALPFLETHKGENPEGFTWTRSDNLARFLTENRSLCRSYLTLARADR
                     LALHGAPGQAQLLQVAAETAGAEAAAFAIFLATRAAASGNKVLLADADDKNQFVFSLL
                     GFADMPPVLTENLQKPSSFRTDLTRCITKAWQGVDYLNLQATSLRCFSDNEISRICGF
                     LDADYDTIIIYSGRRAAPWLSLNAALTFSICAPSLKGEMAALARHAGSPHLVLMRRGE
                     DYYFPWLTPEFGRMVPGDRWQTLPANLNVLAGFVDRALSARRLVIGDGDLGGALSVLN
                     GAALYCRLSGTEASQADKTVNGLQNRLRAYYPRDTFFSARSAKRALARLQLLPVSVLL
                     EINGQPQLVSLPASPELLATAVFPQGVIPALQADNVRVSAAGPGGIDRFRTLALRGQA
                     AEVYSAERLRLQRPNALAALLEQMQP"
     gene            complement(2077..3435)
                     /locus_tag="PLF85_07665"
     CDS             complement(2077..3435)
                     /locus_tag="PLF85_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19580.1"
                     /translation="MDDLVTILLLGGIIGVIAIFGYYVVRTYLVPKRVEELAEMIRQG
                     HTGPAISKLIKMIEENERDPYLHFLLGEAYARQSDFQSAATEYRQTIKISKWSAQVKE
                     AVVRSRLAKIYMQNRNFDEAKKEYLILTRLDGSNNENFYQVGVLFETAGLNDKALPYF
                     KQSAKLKPTHGDSFHHIGIIEYNTGNLNDAKLALTEAVKLNPNLHSAHYYLGMCLKNQ
                     KDYDWAVREFDLASKDETLKGRALLAKGLSLMEKGQLPTAQVELEKGLLTAPKGSELE
                     LNLRYAIAACAERKRDFHTAISNWERITDVNPKFRDVNDKLKAYEEFRTDDTIKDFMI
                     APPGKFEAQSRAIVEALTFKVVDLNVIDDSEVHVLATEEEGNRRNSKKSNRLIYILRT
                     TDMIPERTVRQLHEEMRTKGANKGACISTSEFSSQAHAFIQSRPIELYDRKQLIQLLR
                     AI"
     gene            complement(3437..3820)
                     /locus_tag="PLF85_07670"
     CDS             complement(3437..3820)
                     /locus_tag="PLF85_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19581.1"
                     /translation="MDLEELESLKRENASLRQTIRGYETVVRLNDEEINQLEQMVAMY
                     ERIIEFSRAELIQAREERKASEQLSGLTREELMSAKKTVEASESVSQMSRSELLDALT
                     RIKELEIENKRLKEQRIAAGGKINS"
     gene            complement(3884..4492)
                     /gene="metW"
                     /locus_tag="PLF85_07675"
     CDS             complement(3884..4492)
                     /gene="metW"
                     /locus_tag="PLF85_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNE19582.1"
                     /translation="MELPIVELQTERIDYRIILTLVGAKSRVLDMGCGDGELLYLLNK
                     NLETRSQGIEIDHEQIMQCVERGINVLLGDLDSGLTDFKDNSFDYVILNNALQELRYP
                     DDVIKDALRVGKNLIVGFPNFAYYKARLQFFLSGRTPVTPSLPFAWYESPNLHLLTID
                     DFKAYCAERGFRILSERYVRNQKEIYFRPNLLAQWGIFLITR"
     gene            complement(4494..5621)
                     /locus_tag="PLF85_07680"
     CDS             complement(4494..5621)
                     /locus_tag="PLF85_07680"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNE19583.1"
                     /translation="MSNTASPEQRIFTSLRTFDFPELKLENGEVLKEVRVAYETYGEL
                     NAARSNAILITHAFSGDAHAAFYHEGESRPGWWDNMIGPGKAFDTNRYFIICSNVIGG
                     CAGSTGPSSVNAATGAPYALDFPQITIKDMVNAQKRLVEHLGIEKLYSIAGGSMGGMQ
                     VLQWLVSYPHAMKSAIPIATTARHSPQQIAFNEVGRQAIMADPNWNGGNYYSREIPRA
                     GLSVARMVGHITYMSEKSMAEKFGRRTKSDQDHTKFHADFEVEGYLRNRGDFFVRRFD
                     ANSYLYISKAMDLFDVSEGRPLSEKFYELEAKLLVLAFKSDWLYPAYQSLEIVSACKI
                     AGVPVTYLEIEAHYGHDSFLVETETQSRLVKHFLSRVEVAE"
     gene            complement(5686..6471)
                     /locus_tag="PLF85_07685"
     CDS             complement(5686..6471)
                     /locus_tag="PLF85_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19584.1"
                     /translation="MHRAQIFILGTAGALATIGYLLVALNKDSLGAVSLTTYIGIVVM
                     AVAMKLSGYLITGPRHAIEGKQYGDVMDWAMLLFVWPSYVTPLIVMPLIIGNVGLIFA
                     LFAVGIGLFAFIATWGLKISVDRLRGRRRHAKWFYFIPSFLYSVPALIIGWFVVGRFA
                     SDLTILRVANYGGLLLGAFFSFYCIKLALFDLFTWDVRRYKLMDLVPLVTVVGFVVVI
                     AEIALPLFVGKAEQMYLLYTLSIFALACAQVLGVLSVTRRAKF"
     gene            complement(6471..7070)
                     /locus_tag="PLF85_07690"
     CDS             complement(6471..7070)
                     /locus_tag="PLF85_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19585.1"
                     /translation="MAGVSAQMNGTGDLSHPTVDELHQKLNELVSRFKADEDQKRKNA
                     AAAKTQGDKPKDEGPADEFRIILDRGGKRTAGDKDNPVVLEEVIMKMNGANISSIEMR
                     YEEAAMRRQFMHKRSIKNDDIRKTSYGHLLLYYHAQGDTPKSEVLVNMSSTDRAKLLA
                     IYIDGLLRAIRDLDDRVLAAKKMSGKKMNSALNMGIRIK"
     gene            7199..7534
                     /locus_tag="PLF85_07695"
     CDS             7199..7534
                     /locus_tag="PLF85_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE19586.1"
                     /translation="MDIKVHEKAGYYVVYLKGSLDMYTSVELKSQTDAIPLKTGEKLI
                     LQLNDVSYIDSSGIGVLIKIVNAVQAQSGTVHLTGIKPMIEKIFKVAGLMSFFSFITE
                     EEFKSKYPV"
     gene            complement(7564..>8661)
                     /gene="purB"
                     /locus_tag="PLF85_07700"
     CDS             complement(7564..>8661)
                     /gene="purB"
                     /locus_tag="PLF85_07700"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNE19587.1"
                     /translation="VIAFLTALAENIGPASRHVHFGLTSSDIVDTAQMVLILEAGALI
                     DKALGKTLTALYDFAQKHKDLVVAGRTHGVHAEPTTLGLKFLGHYAELSRSRDRLRHA
                     LDDCRYGKISGAVGTYSQMPPELEAYVLGKFGLNAEPVSTQVIPRDRHAYFLQAIALT
                     GQALYRLMQEIRLLQRTEGRELEEPFQKGQKGSSAMPHKRNPILAERICGLARVLSGY
                     ATTGEYNIALWHERDISHSGAERVILPDATALIEYMLIKSTFVVENLHVHEKNCERVL
                     DATHGLLFSSRALLTVTAKTQMSREDAYAIVQAAAMATWEKPAEGNLRTRLEGDARLA
                     KVTAKDWDEVFDARSFLTHVDTIFARVPKPK"
BASE COUNT         1841 a         2329 c         2476 g         2015 t
ORIGIN      
        1 gctgcagaca ggccatttat ggccgttttg cgagcgattc gcatcgcgaa tcgcggcgtc
       61 tgcagccggc gcgggagttt ttccggaacc tgctagaaaa aattcatcag gtaccagcga
      121 tcgccgcgtt tgcggaagat gaggttgccc atcaggccca tagacgggcg tcccttgaag
      181 tcaatacgca cttcgcattc agtgggcgaa taccagaaga gtcccagctt aatctcgccc
      241 gcacgtgaaa aaaccgtcct gtagctgcgc acgttgtccc caggtgcggt ttctttccag
      301 tacttgtcgt cccagagtac gcggctgaga attgctgatt tgtcatagag cacactgcgg
      361 acctgttcat aactgacgaa ggctttggcg tcaatgattg ctccctcttt ggtattcacg
      421 aagctgagga gcttctcgcg gtcttcggca atgatcgact cgattaccga ggctgccgtt
      481 gcgaccagaa ccgggttctc gcgctgcgct gcggcttcat cggctgcgcc gacaaagaag
      541 tagccctgca gaacgaattc aggcggcttc tggcatgctg ccagccagaa gaggagtata
      601 aatggaattc gtttcatcat cgctgtggcc ttgagcacgg tatttacggc tgcatctgtt
      661 cgagcaacgc ggcgagtgcg ttgggcctct gtaaccgcag gcgctccgca gaatagactt
      721 ccgctgcctg gccgcgcaac gccagcgtac gaaagcggtc gataccgcca ggaccagctg
      781 cgcttacgcg tacattatct gcctgcaggg ccggtataac accctgcgga aaaaccgccg
      841 tcgccagtag ttcgggtgaa gccggcaggc tgaccagctg cggttgaccg ttgatttcca
      901 gtaagacgct gacaggcagc agctgcaggc gggcaagcgc gcgtttcgcc gatcgggcac
      961 tgaaaaaagt atcgcgcggg taataagccc gcaggcggtt ctgcaggccg ttgactgtct
     1021 tgtcggcctg gcttgcttcc gtgccgctca ggcggcaata cagcgccgca ccgttcagaa
     1081 cgctcagagc tccccccaga tcgccatcgc caatgacgag gcgcctggct gacagcgcac
     1141 ggtcgacaaa acccgcaagc acgttcaggt ttgcgggcag ggtctgccag cggtcgccag
     1201 gtaccatgcg gccaaactca ggagtcagcc agggaaaata ataatcttca ccgcggcgca
     1261 taagcacaag gtggggcgag ccggcgtggc gtgcgagcgc agccatttca cctttcagcg
     1321 agggagcaca gatcgagaag gtaagcgctg cattcaggct gagccacggt gccgcgcgcc
     1381 ttcccgagta aatgattatc gtgtcatagt cggcatcgag aaaaccgcag atgcggctga
     1441 tttcgttatc gctaaaacag cgcaggctgg tcgcctgcaa attgagataa tcgactccct
     1501 gccaggcctt ggtgatgcag cgtgtgagat cggtgcggaa tgaagaaggc ttctgcaggt
     1561 tttccgtgag taccggcggc atgtcggcaa agccgagcag tgaaaagaca aactggtttt
     1621 tatcgtctgc atcggcgagc agtaccttat taccagacgc ggctgcgcgt gtcgcgagaa
     1681 agatggcgaa agcagccgct tcggcaccgg cggtctctgc ggctacctgc agcagctggg
     1741 cctggccggg cgctccgtgc agcgccagcc ggtcggcgcg cgccagtgtc aggtacgagc
     1801 ggcagagtga acggttctct gtcaaaaaac gtgccaggtt gtcgctgcgc gtccaggtga
     1861 aaccctcggg attctcgcct ttgtgggttt ctaaaaacgg cagcgcgccg cccaggccgg
     1921 tgataccagc cggtttttca aagcgggcgt aaactgcctc gcccggcagc ggggtagatt
     1981 gccagtcctg tgcctgcaga ctttcgagtt cgccgggcag aattgccttc agcagccggg
     2041 agaaattttc cgcgtcggca gaatcttttg ccacggtcag attgcgcgca gcaactggat
     2101 cagctgcttg cggtcataga gttcgatcgg tcgtgactgg ataaaggcat gtgcctggct
     2161 ggaaaattct gaagtgctga tacatgcgcc cttgttggcg cccttggtgc gcatttcttc
     2221 gtgcagctgc cgcaccgtac gttcgggtat catgtctgtc gtgcgcagta tgtagataag
     2281 ccgattggat ttcttgctgt tacggcggtt accttcttct tcggtcgcca gcacgtgcac
     2341 ttctgagtca tcgatgacgt tgaggtcgac aactttgaag gtgagcgctt cgacgattgc
     2401 ccgcgactgc gcttcaaatt ttccgggtgg agcgatcatg aagtctttaa tcgtgtcgtc
     2461 tgtacggaat tcttcgtagg ccttcagctt atcgttcaca tcgcgaaact ttggattcac
     2521 atcagtgatg cgctcccagt tggaaatcgc cgtgtggaag tcgcgtttgc gttcggcgca
     2581 ggcggcaatg gcgtagcgca ggttgagttc gagctctgac cccttgggtg ccgtcaaaag
     2641 acctttttca agttcgacct gcgcggtggg caactggcct ttttccatga ggcttaagcc
     2701 tttcgccagc agcgcgcggc ctttcagtgt ctcatctttc gacgccaggt cgaattcgcg
     2761 cacagcccag tcatagtctt tctggttctt gaggcacatg cccaggtagt agtgcgcact
     2821 gtgcaggttg ggattcagct taaccgcttc ggtcaaagca agcttcgcgt cgttcaggtt
     2881 gccggtattg tactcaatga tgccgatatg gtgaaatgaa tcgccgtgcg tgggtttcag
     2941 tttggccgac tgcttgaaat agggcagggc tttgtcgttc aggcctgcag tttcaaagag
     3001 cactccgacc tggtagaaat tctcgttgtt tgacccatcg agcctcgtca gaatgaggta
     3061 ttctttcttg gcttcgtcaa aattacggtt ctgcatgtag atcttggcaa gccgcgagcg
     3121 caccacagct tctttcacct gtgcgctcca tttgctgatc ttgatcgttt gccggtattc
     3181 ggtcgcggca gattgaaagt cgctttgccg tgcgtaggct tcgccaagca agaagtgcag
     3241 gtatggatct cgctcgttct cttcgatcat cttaatgagc ttgctgattg ccgggccagt
     3301 atgtccctgc cggatcattt cggcgagttc ttcgacccgc ttgggaacaa ggtatgttcg
     3361 tacgacgtag taaccaaaga tcgcgatgac cccgatgata ccgccgagta gcagtatggt
     3421 tactaaatca tccatctcag ctgtttattt ttccgccggc cgcaatgcgc tgttctttca
     3481 aacgcttgtt ttctatctcg agttctttga tgcgggttaa tgcatcgagc aactcggaac
     3541 ggctcatttg cgagacgctc tccgacgctt ctacggtttt tttcgcgctc atcagttctt
     3601 cgcgtgtaag cccggacaac tgttcactgg cctttctctc ttcgcgcgcc tgtatcagct
     3661 cggcgcggct gaactcgatg atgcgctcgt acatggcgac catctgttcc agctggttga
     3721 tttcttcgtc gttgagcctt accacggtct cgtaaccgcg gatcgtttgc ctcagtgagg
     3781 cattctcacg ctttagggat tccagttcct ccagatccat tggagaagct gaagagcaaa
     3841 tgggattagt gcgtcaagtg tattgcggtc gacgcgcgag gccctaccgg gtgatcagaa
     3901 aaatgcccca ttgggcgagc aaattcgggc gaaaatagat ctctttctgg ttacgcacgt
     3961 accgttcgct gagaatgcga aaaccccgtt cggcacagta ggctttgaag tcgtctatcg
     4021 tcaaaaggtg caggttgggg ctttcgtacc aggcaaaagg gagcgagggt gtgacggggg
     4081 tgcgcccgga taaaaagaac tgcagccgtg ccttgtagta ggcaaaattc gggaacccga
     4141 cgatcaggtt tttgccgacg cgcagggcgt ctttaatgac gtcgtccggg tagcgcagtt
     4201 cctggagtgc attgttcagg atcacgtagt cgaacgagtt gtccttgaag tcggtcaggc
     4261 cgctgtccag gtcacctagc agtacgttga tgccgcgttc gacgcactgc atgatctgtt
     4321 catggtcaat ctcgataccc tgtgagcgtg tctccaggtt cttgttgagc aggtagagca
     4381 gctcgccgtc gccgcagccc atgtcgagca cgcggctttt cgccccgacc agagtcagga
     4441 tgatgcgata gtcaatgcgc tcggtctgta gttcgacgat ggggagttcc atattattcg
     4501 gccacttcaa cgcgggagag aaagtgtttc accagccgcg actgtgtttc cgtttcgaca
     4561 agaaaagagt catggccata atgcgcctcg atttccaggt aggtcacggg tacaccggcg
     4621 atcttgcacg ctgaaacgat ttcgagcgac tggtaggccg ggtaaagcca gtcagacttg
     4681 aaggccagga caagcagttt tgcttcgagc tcataaaact tttcgcttaa tgggcggcct
     4741 tctgagacgt caaaaagatc catcgctttg ctgatgtaga gatacgaatt tgcatcaaag
     4801 cgccggacaa aaaaatcccc ccggttgcgc aggtaacctt cgacctcgaa gtcagcgtgg
     4861 aacttggtat ggtcctggtc agactttgtg cggcggccga atttttcagc catcgatttt
     4921 tcactcatgt aggtgatgtg cccgaccatg cgggcaaccg aaagaccggc gcgcggtatc
     4981 tcgcggctat agtagttgcc gccattccag ttggggtcag ccatgatcgc ctgccgacct
     5041 acctcgttga aggcgatctg ctgcggcgaa tggcgtgcag tcgtcgcgat aggtatcgcc
     5101 gatttcatgg catgcgggta acttaccagc cactgcagaa cctgcatgcc gcccatcgag
     5161 ccgcctgcaa tcgagtataa cttttcaata cccaggtgct ccaccagacg cttctgcgca
     5221 ttcaccatgt ctttgatggt aatctgtgga aaatcgagtg cataaggcgc gccggttgcc
     5281 gcgttcaccg acgaggggcc ggtcgaaccc gcgcagccac caatgacatt cgaacaaata
     5341 ataaaatagc ggttcgtatc aaatgctttg ccgggaccaa tcatgttgtc ccaccagcca
     5401 gggcggcttt caccttcgtg gtaaaacgcc gcgtgcgcgt cgccggaaaa cgcgtgcgtg
     5461 atcaggattg cattgctgcg cgcggcattc agctcgccgt aagtttcgta agcaacacgt
     5521 acttctttca ggacttcgcc gttttccagc ttgagttcag gaaagtcaaa ggttcgcagg
     5581 ctggtaaaga ttcgttgctc aggcgatgcg gtgttactca ctgttggtat tgcgcctgca
     5641 cactatcagg ctgcaccccg acgcgtaagg tgcacaggct gtcattcaaa actttgcccg
     5701 gcgcgttact gacaggacgc cgagtacctg tgcgcaggcc agggcaaaaa tactcagcgt
     5761 atagagcagg tacatctgtt cggctttgcc gacgaaaagc ggcagcgcga tttctgcaat
     5821 gacgacgaca aaaccaacaa cggtcacgag cggtacgaga tccatcagct tatagcggcg
     5881 gacatcccac gtgaacagat cgaagagagc gagcttgata cagtagaagc taaagaaagc
     5941 tcccagcagc aggccaccat agttcgctac gcggagaatc gtcaggtcag aggcaaacct
     6001 gccgacgaca aaccagccaa taatcaatgc cggtacagaa tacaggaatg aagggataaa
     6061 atagaaccac ttcgcgtgcc gccggcgtcc ccgaagacga tccacgctga tcttgagacc
     6121 ccaggtagcg atgaaggcga agaggccgat gcccactgca aaaagggcga agatcagccc
     6181 cacgttacca ataatgaggg gcatgacgat cagcggcgtc acgtaactcg gccagacgaa
     6241 gagcagcatc gcccagtcca tgacgtcgcc gtactgcttg ccctcgatgg catggcgcgg
     6301 gccggtaatc agatagccag aaagtttcat cgccacggcc atgacgacga taccgatgta
     6361 ggtcgtgagc gagacagcgc cgagcgagtc tttattgagc gcaaccagaa ggtaaccgat
     6421 cgtcgcgagg gccccggcgg tgccgagtat aaaaatctgc gcccggtgca ttacttgatg
     6481 cgaatgccca tgttgagggc gctgttcatc tttttgccag acatcttttt cgccgccagg
     6541 acgcggtcgt caaggtcacg tatggccctg agcaggccgt cgatatagat cgcgagcagt
     6601 ttggcccggt ctgttgaact catgttcacc aaaacttctg acttgggtgt atcgccctgc
     6661 gcatggtagt agagcagcag gtggccatag ctggttttac ggatgtcatc gttcttgatg
     6721 gaacgtttgt gcatgaactg gcggcgcatg gcggcctctt catagcgcat ctcgatcgaa
     6781 gagatgttcg caccgttcat cttcatgatg acctcttcga ggacgaccgg gttgtctttg
     6841 tcgccggctg tgcgcttgcc gccacgatcg agaataatgc ggaattcgtc tgcaggaccc
     6901 tcgtctttgg gcttatcacc ctgggtcttg gccgccgcag cattcttgcg tttctggtct
     6961 tcgtctgcct tgaaccggct caccagctca ttcagttttt ggtgcagttc atcaaccgtc
     7021 ggatggctga ggtcgcccgt gccgttcatc tgggcagaca caccggccat gggaacagcc
     7081 agggataggc aaaataaaat aaatgcttgg cgtctcatgg taatctatta tcgacgtgac
     7141 agatgcagat atgtagtgta aacgatattt acaccacccg cagatgagag gttattgaat
     7201 ggatattaag gttcacgaaa aagcgggata ttatgtcgtc tacctaaagg gcagcctcga
     7261 catgtatact tcggtcgagc tcaagagcca gactgacgcg atcccgctca aaaccggcga
     7321 aaagctgata ctgcaactca atgacgtctc ttacatcgat tccagcggta ttggagtgct
     7381 catcaagatt gtgaatgcag tccaggcaca gagcggtacc gtgcacctga ccggcatcaa
     7441 gccgatgatt gaaaagatct tcaaagtcgc cgggttaatg agtttcttct cttttattac
     7501 tgaagaagaa tttaagtcga aatatccggt ttaaatgatc aatccggcct gcggccggat
     7561 tgatcatttg ggcttgggca cccgcgcaaa gatcgtatct acgtgggtca aaaacgaccg
     7621 ggcgtcgaaa acctcgtccc aatccttcgc cgtaactttt gccagtcgcg catcgccttc
     7681 cagtcgagtt ctcaggttgc cttctgccgg tttttcccag gttgccatcg ctgccgcctg
     7741 cacaattgcg taggcgtctt cgcggctcat ctgggttttc gcagtgacag tcagtagtgc
     7801 ccgcgacgag aacagcaggc catgcgtcgc atcgagcacg cgctcacagt tcttttcatg
     7861 cacgtgcagg ttctcgacca caaaggtcga cttgatgagc atgtattcga tgagtgccgt
     7921 tgcgtcgggc aaaatcacgc gctcagcgcc ggagtgcgag atgtcacgtt catgccatag
     7981 cgcgatgttg tattcgcccg tggtggcata acccgaaagt acgcgcgcga gaccgcagat
     8041 gcgttcggca agaatcgggt tgcgcttgtg cggcatcgcg ctcgaacctt tttgcccttt
     8101 ctggaatggt tcttcgagtt cgcggccttc ggtacgctgc agcagtcgaa tttcctgcat
     8161 caggcggtag agcgcctgcc cggtgagtgc gatggcctgc aaaaagtacg catggcggtc
     8221 gcgggggatt acctgcgtgc tgacgggttc ggcattaagg ccgaattttc cgagcacata
     8281 tgcctcgagc tcgggcggca tctgcgaata ggtacccacc gcgccggaaa tttttccgta
     8341 gcggcagtcg tcgagcgcgt ggcgcagcct gtcgcgagaa cgcgaaagct ccgcatagtg
     8401 gccaagaaat tttaaaccca gggtcgtcgg ctctgcatgt acgccatgtg tgcgacccgc
     8461 tacgacaaga tctttgtgtt tttgcgcgaa gtcatacagc gccgtgagtg ttttacccag
     8521 cgccttgtcg atcagcgcgc ccgcttcgag aattagcacc atctgcgcgg tgtcgacaat
     8581 gtcagatgac gtgaggccaa agtgtacatg gcgcgatgcc gggccgatat tttcggcgag
     8641 cgctgtgaga aaggcaatga c
//
LOCUS       DAOQJF010000117         8626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4390_length_8626_cov_4.936180, whole genome shotgun sequence.
ACCESSION   DAOQJF010000117 DAOQJF010000000
VERSION     DAOQJF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4390_length_8626_cov_4.936180"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1152)
                     /locus_tag="PLF85_07705"
     CDS             complement(178..1152)
                     /locus_tag="PLF85_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19588.1"
                     /translation="MRSAKYLAAALLLSSSAVHPIRWEPGHEDRRTDKWDLMVTDPIT
                     TDSCNMTAKGLFVLQNFTLMNITRARFDGTGNKQPIDSSVADQNLTLNHQLTYGVTEK
                     WEIDAIPFPIVFNDNTREVDGPRVGLGDASAWSRYLIFEQTHEQKYIPSLLFFTMVKV
                     PTGIVDLQDKTGRRTFGNLMTEVHAGPNLMWEFRPIVINLNFWYVGTFGRFFHAPSGT
                     IAENPGDMLEMNASVEWVVRDYQNDSSVIFAEVVQSYHGRFKALGNRETDQPVAYNLQ
                     ATIGFAEFIDRNTFLIVSLAVTLYGQNTYQNWGPYISAGKTFHFTNSP"
     gene            complement(1139..2905)
                     /gene="leuA"
                     /locus_tag="PLF85_07710"
     CDS             complement(1139..2905)
                     /gene="leuA"
                     /locus_tag="PLF85_07710"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNE19589.1"
                     /translation="MKPEIVKKYQPYRPVDLKDRTWPNKSITTAPRWCSVDLRDGNQA
                     LVYPMEIPEKAELFNLLVAVGFKEIEVGFPAAADVEYNFLRYLVDNKLVPNDVTVQIL
                     TQAREHLIRKSFDALAGVHRAIVHMYNSTSTLQRDVVFGMSREQIKQLAVDGVKLVKQ
                     LAVEYHKKTGTIFDLEYSPESFTGTEVDFAVDICAAVMDVWEPTEQSKVILNLPSTVE
                     MTTPNLYADQIEYFVRNIPRRDLAVISLHTHNDRGCGVAASELGLMAGADRVEGTLFG
                     NGERTGNCDLVTVALNMLTQGVDPKLDFSNINQVAEVYERTTRMDIHQRHPYVGELVF
                     TAFSGSHQDAINKGLAKRKQAEHEGKAITWAVPYLPIDPHDIGRTYESIIRINSQSGK
                     GGVAYVMEHDFGCRLPKTMHPEFARVVQVIAEKQGGEVMPAEINTAFENEYLKRKSPL
                     ELVGISAIEEKPDSGEVNLRLKVHSDKPGFLASGNAEYEGRGNGPLDAARNAIISAIS
                     PLPASPQTWGEVPKAEGGVFSFQIKNYSEHSLGEGSDARAIAYIQIERPDKATFYGVG
                     VSPNISRASIEALFSAVNRAFG"
     gene            3242..4018
                     /locus_tag="PLF85_07715"
     CDS             3242..4018
                     /locus_tag="PLF85_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19590.1"
                     /translation="MLLISPIVASDDGSTEAGPEPVVERSAAEYKGGVSALTKPELPL
                     FSLHFVIMTDHPKAVAGATPERLQQIEDLLNEKFAATTGEHLARFKIRSFASLEVIRN
                     SACPEIYESIVNRTSTRYGDYWKCKDLNVRAPQGITIFIKHHDKDKLSYGGQVNGMPF
                     AQINWERFLKDQRVFLHELGHAFGLSHSCPPKKGSGGRPNVMNFIKGCKELPEGEAGW
                     LYTPSQAAAVKKRRDGYVKKFAKTKDPVWEKVLAESSLVR"
     gene            4116..4367
                     /locus_tag="PLF85_07720"
     CDS             4116..4367
                     /locus_tag="PLF85_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNE19591.1"
                     /translation="MAMKRTQVYLTQNEHKALTALSRKTKKKRSEIIREAIDEHLEKQ
                     LNKKTRAQILDEVAGSWKDNDVDFDALRKSWNRKIETFD"
     gene            4351..4722
                     /locus_tag="PLF85_07725"
     CDS             4351..4722
                     /locus_tag="PLF85_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE19592.1"
                     /translation="MKLLIDTNVLVDQLRGFKSAVAFGSALPEDTAISVITVAEIHAG
                     VRSEQQHNRAAALIETYQILQLDAISAQMAGHFLRKFAKSHSLNFADAAIAATAATHH
                     LQLITLNTKHFPMFPGLKKPY"
     gene            complement(4758..6470)
                     /locus_tag="PLF85_07730"
     CDS             complement(4758..6470)
                     /locus_tag="PLF85_07730"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNE19593.1"
                     /translation="MSLTPEQYYQKFLQSPLTAAEKDDLEKNKAQLISERAFDGEPAF
                     GTGGMRAITGLGTNRLNVQNIGRLNLAVAEYFKKTKSSPLLAMGYDSRLTSPQFSRLS
                     YHILTQNGLRVKIFRRPTPTPLLSFAVRELGADCGVVLTASHNPPQYNGYKAYGADGG
                     QIISPVDKDIQNLFLATGFDRLPANLHELAEKPIPEADLIEEETYQAYIARLKKEVFY
                     QNKPKHARILYSPLHGTGGWLFERAFKELGYNTFSVLAEQAQPDGNFPTVKSPNPEEP
                     AAFERLLAAANGATEMPDLLVATDPDADRVGAMLKTATGYHFLTGNQIGSLLLESIAR
                     KKTKSLAKPYICKTIVTTELQRLIADGYGIKTVETLTGFKYIAEQVAKDPENYLFGGE
                     ESFGYLPIHWVRDKDSLSSALALAELAEDENLVQALDEIYIRHGLFHELLHSIDLSKN
                     PALLPETLGKLADPQAFAQQVNFGREVVDILDLRKGAKEPATQTCRDLKKQLGEGDVI
                     QFWLKDFSRLTIRPSGTEPKIKAYLSLMGKQKPTAASLEADKVALKSEAEGILKKFLT
                     ALGV"
     gene            6622..7668
                     /locus_tag="PLF85_07735"
     CDS             6622..7668
                     /locus_tag="PLF85_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19594.1"
                     /translation="MYSFSEIKQVHLEVTERCNAACPQCPRRIEGGVLNPRLTMAELS
                     LAQVQQLLAPEFLRQLKKIYLCGNYGDAAAAGDTLGIIRYLRENSPNLQIGLHSNGSL
                     RNTAWWAELAALISQNGYARFAIDGLEDTNHIYRRNTDWQTIMVNAKAFIAAGGRAEW
                     DFIVFAHNEHQVEAARALAAEMGYAAFNVKRTARFLKREYVSADAPSPVRQRSGAVVS
                     ELAPPVSTEHRHPVADALQSAAEQQQSYAEFLMNREISCQVAQSKSLYISARGYVLPC
                     CWLAGMLHNPESTETRQFATEFPDLEATGSVDGLKKSLEDIVMGDFFQRAVSSRWPKG
                     STGRLEVCARVCGN"
     gene            7668..8579
                     /locus_tag="PLF85_07740"
     CDS             7668..8579
                     /locus_tag="PLF85_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE19595.1"
                     /translation="MGHSHDHGHGHGHGHHHHDHASRNLLVALLLNLGFAAIELVGGI
                     YTNSVAILSDALHDFGDAFSLGVAWYLQKISGKPRDEYYSYGYRRFSLLGALVVSSVL
                     LIGVALVIRESVERLMKPEQADAKGMLYLAILGVAINGYAALRVKKGESHNERAVYLH
                     LLEDVLGWIAVLISSIVMIFFNVPFLDPLVSLGISLFILFNVYKNLRGVVRILLMEVP
                     GHVQLPEFESAVRALKHVKDLDDLHLWTLDGQNHVLTMQVIVSDKLSFSVLRRLKQQI
                     RQLAELQGIRHSTIEFCCHGEDRHQHH"
BASE COUNT         1902 a         2386 c         2410 g         1928 t
ORIGIN      
        1 aaaaacttcc gtgcggagta cgagcgccga cagaacggcg tttcagaaga cgaagtcgac
       61 gagtaccgcc gcaagaaaca ggcgctgggc tttttcgtat cgccgacgat cagcaacggc
      121 cgctacgccg cgtcggtgac gtggtattgg tagcgtgcgg cctgtgagag ctgcccgcta
      181 cggcgaattt gtgaaatgga aggtcttacc cgcagaaata taaggacccc agttttggta
      241 agtattctgg ccgtagagag taactgccag tgaaacgatg agaaaagtgt tccgatcgat
      301 gaactcggca aagcctatgg tcgcctgcag gttgtacgcg acgggctggt cggtctcccg
      361 gttgcccagc gccttgaacc ggccgtggta tgattgcacg acttctgcaa aaatcacgga
      421 cgaatcgttc tggtaatcgc gcaccaccca ttcaaccgac gcgttcattt cgagcatatc
      481 tcctggattt tcggcaatgg tgcccgaggg tgcatggaag aagcggccga acgtgccgac
      541 ataccagaag ttgaggttaa tgacgatggg cctgaactcc cacattaggt tggggccggc
      601 gtggacctcg gtcatcaggt tgccgaatgt gcggcgcccg gttttgtcct gcaggtcgac
      661 gattcccgtt ggcactttca ccatggtgaa aaacagcagc gaaggaatat atttctgctc
      721 atgcgtctgc tcaaagatca ggtagcgcga ccacgccgac gcatcgccca ggccgacacg
      781 cggtccatcg acctcacgcg tgttgtcatt aaaaacaatg gggaatggaa tcgcatcgat
      841 ttcccacttt tctgtaacgc cgtacgtcag ctggtggttg agcgtcagat tctggtcggc
      901 tacgctcgaa tcgatcggtt gtttgttgcc tgtcccgtca aagcgggcac gcgtgatatt
      961 catcagtgtg aagttctgaa gaacaaacaa ccccttcgcc gtcatgttac acgaatcggt
     1021 ggtgatgggg tcggtcacca tgagatccca tttatcggta cggcggtctt cgtggccggg
     1081 ctcccagcga atgggatgta cagcgctgct ggaaagcagc agcgctgcag ccaggtactt
     1141 agccgaacgc acgattcaca gcggaaaaca gcgcttctat cgaggcgcgt gagatattcg
     1201 ggctcacacc cacgccgtag aatgtcgcct tgtcgggccg ctcgatctga atgtaagcaa
     1261 tcgcccgcgc atcagaccct tcacccagcg aatgctcgct gtagttcttg atctggaagg
     1321 agaaaacgcc cccctccgcc ttcggcacct ccccccaagt ttggggggag gccgggaggg
     1381 gggagattgc cgaaataatc gcgttacgcg cagcatcaag cgggccattg cccctgccct
     1441 cgtattcagc gttgcccgaa gccaaaaagc ctggcttgtc ggagtgaacc ttgagcctga
     1501 gattcacctc gccactgtct ggcttttctt cgatcgccga aataccgacg agctcgagcg
     1561 gtgatttacg tttcaggtat tcattttcaa aggcggtgtt gatttcggcc ggcatcactt
     1621 cgccgccctg cttttcagca atcacctgca cgacgcgggc aaattcaggg tgcatcgttt
     1681 tgggcagccg gcagccgaaa tcgtgctcca tcacgtacgc aacgccacct ttgccagact
     1741 gcgaattgat gcggatgatc gattcgtacg tgcgtccgat gtcgtgcggg tcgatcggca
     1801 aatagggcac cgcccaggta atggctttgc cctcgtgctc ggcctgcttg cgcttggcca
     1861 ggcccttgtt gatcgcgtcc tggtgcgaac ccgagaaggc cgtgaagacc aattcaccga
     1921 cgtatggatg gcgctggtga atatccatgc gcgtggtgcg ctcataaact tcggcgacct
     1981 ggttgatgtt cgagaaatcc agtttcggat caacaccctg cgtcagcatg ttcaatgcca
     2041 cggtcacgag gtcgcagtta cccgtgcgtt ccccgttacc aaagagggta ccttcgacgc
     2101 ggtcagcacc cgccatcaga cccagttctg aagccgcgac accgcagccg cggtcgttgt
     2161 gtgtgtgcag gctgatcacc gctaagtcac gccgcggaat gttgcgcaca aaatattcaa
     2221 tttggtcagc gtagaggttg ggtgtcgtca tctcaaccgt gctcggcagg ttcagaatga
     2281 ctttactctg ctctgtcggc tcccagacgt ccatcactgc ggcgcagatg tcgactgcaa
     2341 aatcgacttc ggtgccggtg aaactttcgg gtgaatactc gagatcgaag atcgtgccgg
     2401 tcttcttgtg gtactcgacc gcgagctgct tcaccagttt taccccgtca acggccaatt
     2461 gtttaatctg ctcgcggctc atgccgaaca cgacatcacg ctgcaaggtc gaggtggagt
     2521 tatacatgtg gacgatggcc cggtgcacac cggccaatgc gtcgaaactc ttacggatca
     2581 aatgctcgcg tgcctgcgtc agaatctgta ccgtgacgtc gttcggcacg agcttgttgt
     2641 cgaccaggta gcgtaagaaa ttgtactcca catcggccgc cgccggaaag ccgacttcga
     2701 tctctttgaa accgactgca acgagcaggt tgaagagctc tgctttctcg ggtatttcca
     2761 tcgggtagac gagcgcctgg ttgccatcgc gcaggtcgac gctgcaccag cgtggtgctg
     2821 tggtaatgga tttattgggc caggtgcggt ctttcagatc gaccgggcgg tagggttggt
     2881 atttttttac gatttcaggt ttcatattgc ctccaagtgc tgattatctg gcgcttgctg
     2941 cgccgcgatg ccatagggtt cggttgaatg aactaaccga cctatggcac attatagtcg
     3001 gggcaatagt cttagacagc gcgaccggag gccgacgggg tgaatgccga tcagagattg
     3061 tgtagaagtg gcagttgcca tcggcatatt cctaatatat acatcttcga caagccggca
     3121 agttttttga agatctcgcc gtgttcgccg cgccgccgcg gttcaattat caattgattg
     3181 cagaatcgag gcccctgcgc aaactgctac catgcatttc ctgctcaaaa ccgccctcct
     3241 tatgttgctt atttccccga ttgtggccag cgacgatggc tccactgaag cgggacctga
     3301 gccggttgtt gagcgctctg cggcggaata caaaggcgga gtgagtgcgc tcacgaaacc
     3361 tgaactcccc ctattctctc tgcactttgt catcatgacc gaccacccga aggccgtagc
     3421 cggcgccaca cccgaacgcc tgcaacagat tgaagatctg ctcaacgaaa agtttgccgc
     3481 aacaacgggt gagcaccttg ctcgcttcaa gatacgatca tttgccagcc tcgaagtgat
     3541 caggaactct gcctgccctg aaatttacga gtcgatcgtt aaccgcacaa gcacccgcta
     3601 cggcgactac tggaaatgca aagacctgaa cgtgcgcgca ccgcagggaa taacgatttt
     3661 cattaagcac cacgataaag ataaactctc ttacggcggc caggtcaatg gaatgccatt
     3721 tgcgcaaatc aactgggagc gctttctcaa agaccagcgc gtctttttgc acgaacttgg
     3781 tcacgccttt ggactttcgc acagctgccc gcccaaaaaa ggttcaggtg gccggccgaa
     3841 tgtgatgaat ttcatcaaag gctgtaaaga gctgcccgag ggtgaagcgg gctggctcta
     3901 tacaccctca caggcggcag cggtcaaaaa gcgccgcgac ggttatgtaa agaagttcgc
     3961 gaaaacaaaa gacccggttt gggagaaggt gttggcagag agcagcttgg tgaggtaggc
     4021 gtttcgccga agctatcctc aacgcgtttc attgatgcgc cagcgaggcg cccgatcccg
     4081 attgacacga tgtatacacc gacatatatg tggctatggc catgaagcgc acgcaggtat
     4141 acctgactca gaacgagcac aaggcattga ccgccctcag ccgcaagacc aagaagaagc
     4201 gcagcgaaat catccgcgag gcgatcgacg agcatcttga aaaacaactc aacaagaaga
     4261 ctcgggcgca gatacttgat gaggttgcag gctcgtggaa agacaacgat gttgatttcg
     4321 atgccctgag aaagtcgtgg aaccgcaaga ttgaaacttt tgattgatac caacgttctg
     4381 gtagatcaac tgagaggatt caaaagcgcg gtcgcattcg ggagcgccct gcctgaagac
     4441 accgcgattt cggtcatcac ggtcgccgag atccatgcag gtgtccgatc agagcagcaa
     4501 cacaaccgag cagccgcgct gatcgaaacg taccagatac tgcagctcga tgcaatcagc
     4561 gcgcaaatgg ctggccattt tctgcgcaaa tttgcaaaat cacactctct caattttgcc
     4621 gatgccgcga ttgcggccac cgcagctaca caccaccttc aactcatcac cctgaacacc
     4681 aaacacttcc cgatgtttcc aggcctcaag aagccgtatt gagtggttat gggtcaccgg
     4741 ttggtgactc ataatcctta caccccaagc gcggttaaga acttcttcag tatgccctct
     4801 gcctcgctct tgagtgcaac cttgtcagcc tcaagcgatg ccgcagtcgg cttctgttta
     4861 cccatcaggc tcaggtatgc cttgatcttc ggttcagtac cagacgggcg gatggtcagc
     4921 cgcgagaaat cttttagcca aaactggatg acatccccct cgcccagctg ctttttcaga
     4981 tcacgacaag tctgcgtcgc tggttctttg gcacccttgc gcaagtcaag aatgtcgacc
     5041 acttcacggc caaaattcac ttgttgtgca aatgcctgtg gatcagcgag tttgccgagc
     5101 gtctcaggca acagcgcggg gtttttgctc agatcaatgc tgtgcagcaa ttcatgaaac
     5161 agcccgtggc ggatgtaaat ttcatcgagc gcctgcacga ggttttcatc ttccgcaagt
     5221 tcagccagcg cgagcgccga cgagagcgaa tctttgtcgc gcacccagtg gatcggcaga
     5281 tagccaaaac tctcttcgcc gccgaaaagg tagttttcgg gatctttcgc gacctgctcg
     5341 gcaatgtact taaagccggt gagcgtctcg actgttttga tgccataacc gtcggcaatc
     5401 aagcgctgca gttcggtcgt gacgatagtt ttgcagatat agggttttgc cagcgacttg
     5461 gtctttttgc gcgcgatgct ttcgagcaga agcgaaccaa tctggttgcc ggtgagaaaa
     5521 tggtagcccg tcgccgtctt caacatcgcg cccacgcggt cggcgtcggg gtcggtggcg
     5581 accagcaggt ctggcatctc tgttgcgcca ttggctgcag cgagcagccg ttcgaatgcc
     5641 gccggctctt cagggttggg tgactttacc gttgggaaat tgccatcggg ttgagcttgt
     5701 tctgccagca ctgaaaaggt attgtaaccc agctctttga atgcacgctc aaagagccag
     5761 ccacccgtgc cgtgcagcgg tgaatagaga atgcgtgcat gctttggttt gttctggtag
     5821 aagacttctt tcttcaggcg cgcgatataa gcctggtaag tctcttcttc tataaggtcc
     5881 gcttcgggta tcggcttttc tgccagctcg tgcaggttcg ccggcaatct gtcaaagccc
     5941 gtcgcgagaa agagattctg gatatcttta tcgacaggtg agatgatctg cccgccatcg
     6001 gcgccgtatg ccttgtagcc gttgtactgc ggcgggttat gcgacgcggt gagtaccaca
     6061 ccacaatcgg cgcccagttc gcgcacggcg aaagacagca atggtgtcgg cgtcgggcgg
     6121 cgaaagatct tcacgcgcag gccattttgc gtcaaaatat ggtagctcag ccggctaaac
     6181 tgcggcgaag tcagccgtga atcataaccc atcgccaaaa gcggtgaact cttcgttttc
     6241 ttgaaatatt cagccacggc gaggttaagg cggccgatgt tctgcacatt gagccggttg
     6301 gtgccgagcc cggtaatcgc gcgcatgccg cccgtaccga acgcgggttc gccgtcgaac
     6361 gcgcgctcac taatgagctg cgctttattc ttttcgaggt catccttctc ggcggctgtc
     6421 aggggggatt gcagaaactt ctggtagtat tgctcggggg tgagagacat cccccttgtt
     6481 ttggggcaaa aatgccggtg taaagcagaa acaatacctc tttgatttgc ccccgaatta
     6541 attcgggggc aaatcaaagg caattgcgga atttcattgt taaccgcgta gagacgccat
     6601 gaatggcgtc tctacatttc tatgtattca ttttctgaaa taaaacaggt tcacctcgag
     6661 gttaccgaac gctgcaatgc cgcctgcccg caatgccccc ggcgtatcga gggtggcgtg
     6721 ctgaacccgc ggctcaccat ggcggagctt tcgctggcac aggtgcagca actgctggcg
     6781 ccggagtttc tgcggcagct gaaaaaaatt tacctgtgtg gcaactacgg tgacgctgcg
     6841 gcggccggcg acacgctcgg catcattcgc tacctgagag aaaactcccc caacctgcag
     6901 attggcctgc actcgaatgg cagcctgcgt aacacggcgt ggtgggcaga gctcgcggcg
     6961 ctgatcagcc agaacggtta tgcgcgtttc gcaatcgacg gtctcgaaga caccaaccac
     7021 atctatcgcc gcaacaccga ctggcagaca atcatggtaa atgcaaaggc cttcattgca
     7081 gcaggtggcc gcgccgagtg ggatttcata gtctttgcgc acaacgagca ccaggtcgaa
     7141 gcagcgcgcg cgctggcagc agaaatgggt tatgccgcct tcaacgtcaa acggacagca
     7201 cgttttctca aacgcgaata tgtcagcgcc gatgcccctt ctcctgtgcg ccagcgcagt
     7261 ggcgcggtgg tttcagaact ggcgccaccg gtaagtactg agcaccggca tccggtagct
     7321 gatgcgctgc aaagtgcggc ggagcaacag cagagttatg cagaatttct gatgaaccgc
     7381 gagatcagct gccaggtagc gcagagcaag agcctctaca tctcggctcg gggttatgtt
     7441 ttgccctgct gctggctcgc aggcatgctg cacaaccccg aaagcacaga aacacggcag
     7501 tttgccacag aatttccgga cctggaagct acgggaagcg ttgacggcct gaaaaaatct
     7561 ctggaagata tcgtcatggg agacttcttc cagcgtgcag tgagcagccg gtggccgaaa
     7621 ggcagcacgg gccggctcga ggtctgcgcg cgggtgtgcg gcaactgatg ggacacagtc
     7681 atgatcacgg gcacgggcac gggcacggcc atcaccacca tgaccatgcc tcgcgtaacc
     7741 tgctcgtagc gctgctgctc aacctcggct ttgcggcgat tgaactcgtc ggcggcatct
     7801 acaccaacag cgtcgccatc ttatccgatg cgctgcacga tttcggcgac gccttttcgc
     7861 tcggcgtcgc gtggtacttg cagaaaattt cgggcaagcc gcgtgacgaa tattactcct
     7921 acggctaccg tcgattctct ctgttaggag ctcttgtggt ttcgtcggtg ctgctgatcg
     7981 gcgtggcact ggtgatacgc gaatcggtcg agcggctgat gaaacccgaa caagccgatg
     8041 cgaaaggtat gctctacctc gcaatactgg gtgtggcaat caacggctat gctgcactgc
     8101 gcgtcaaaaa aggcgaatcg cacaacgaac gcgcggttta cctgcacctg ctcgaagatg
     8161 tattgggctg gattgcggtt ctgataagca gcatcgtgat gatttttttc aacgtgccgt
     8221 ttctcgaccc gctggtttcg ctcggtattt cgctctttat actctttaat gtctataaga
     8281 acctgcgcgg cgtggtgcgc attctgctga tggaggtacc aggtcatgtg cagctgcccg
     8341 agtttgaatc ggcggtccgc gcgctgaaac atgtcaagga cctcgacgac ctgcacctct
     8401 ggacactcga cgggcaaaac cacgtgctga cgatgcaggt gatcgtctct gacaagctga
     8461 gtttttcggt tttgcgcagg ctcaagcagc agatacgcca gctcgccgaa ctgcagggga
     8521 ttcgtcacag cacgatcgag ttctgctgcc acggcgaaga ccgccaccag caccactaag
     8581 gcgagaacga tgagagcagt attcacctta ctcatcttca gccccg
//
LOCUS       DAOQJF010000118         8609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4401_length_8609_cov_4.871405, whole genome shotgun sequence.
ACCESSION   DAOQJF010000118 DAOQJF010000000
VERSION     DAOQJF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4401_length_8609_cov_4.871405"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="argB"
                     /locus_tag="PLF85_07745"
     CDS             complement(<1..656)
                     /gene="argB"
                     /locus_tag="PLF85_07745"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNE19596.1"
                     /translation="MGSNENSHDRIGTLVEALPYIQRFAGKTLVIKYGGAAMDKADLS
                     DRFAQDVVMLKACGIDPVIVHGGGPMINQLLSRLGVKSEFIQGHRVTGDEEMAAVEMV
                     LGGDVNKRIVAQIARKGGKAVGISGRDGALARATRHKIEHKTEQGTELIDLGRVGSVE
                     PRDVNVQVLNALKAGGFIPVIAPVAFDDDGNSLNINADTMAGAIASALGAEKLLLLTD
                     T"
     gene            complement(664..987)
                     /locus_tag="PLF85_07750"
     CDS             complement(664..987)
                     /locus_tag="PLF85_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNE19597.1"
                     /translation="MVLLFGMPNMGEMIFLVVIILLLFGGKKLPELARGLGAGIHEFR
                     RGMNQGNAPVEAQEHVPERDITPHTHEREESHRPKSIPAPKARGGRPSARRTATPKKK
                     AAKKK"
     gene            1118..1612
                     /locus_tag="PLF85_07755"
     CDS             1118..1612
                     /locus_tag="PLF85_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19598.1"
                     /translation="MKLPFGLFTSCCVWAVVILGGAAGCGKKAEPVAASGSELPPAAK
                     PGDAPEFIRSLSLLYPQAELYRAEAAEKNVGKAGIILQKTNHPLEDVVRYYGDTLRKH
                     GFTETTHLVQTNGALLQYERRADKNADKRELVSVDISKLPYAENLLIRVGRSETDYSR
                     GGNP"
     gene            1609..2736
                     /locus_tag="PLF85_07760"
     CDS             1609..2736
                     /locus_tag="PLF85_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE19599.1"
                     /translation="MKKTEPREVQLDRSYSFLDFHRFLPYVFFGLFLFLLGLVSFVYY
                     SYIYAFFISGILFILFRTPHRWLLSKLDGRRTMAAAISTVFVILSLIIPAIFLAIALF
                     TEARHASELAREWFTPEKLFEIHRNNPWIEGRLDISAQQLLAYRLQLLDAIRNNQLET
                     LKQGWTWATTGLKFFVDFTFALFILFFLFRSVDRIGLAVYRNLPFPDKIEKHIGDRMV
                     QIFDAVVKGNLVISMVQGSVIGIVFWLCDLSTPLLWGAVAAPFALIPVIGTAVVWLPG
                     ALYLYTHNQETTAVVMGITCLVAYFLLENLVKPLLLDKDLNLHPLFLFLAIVGGLNAF
                     GIKGLILGPFIVTMFVTIWELISEWNRNFSEAEGGGETPSE"
     gene            2764..3561
                     /locus_tag="PLF85_07765"
     CDS             2764..3561
                     /locus_tag="PLF85_07765"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNE19600.1"
                     /translation="MPLRKKILYAGLSLFALGGILLTWYVVKRHSGDGEWAVSVEGNR
                     ILTEKDIQDVVTYLLRAAKDGVSADEIRDALLLNTRIATARVAVLPGQRIQIAITERT
                     LEYLQNEGNGVAEKDGKGTTIVENASQIHRDFTPDKVIFYLTFGGESMTSPRSDIIRL
                     WKDTQGKYAFLWQRLAEIEIQPLKDRTTDETGKPGIWRYRIYSAGIRSCVVYEGRFSE
                     ETLRRLWAVYAYLEAKLPRSVTLVDLQENSAIIREMKTNRSGTEGDT"
     gene            3558..4793
                     /gene="ftsA"
                     /locus_tag="PLF85_07770"
     CDS             3558..4793
                     /gene="ftsA"
                     /locus_tag="PLF85_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNE19601.1"
                     /translation="MSYDAGSHGGRIVAALDLGSSKTVAIVARVSSPEDIEIIGLGVV
                     EARGVKSGSVTNIELTVKSIRDAIEEAELMSAVEIDEVIINISGKNVRGDNANGMVTI
                     TGRDRIISHADVFRVIEQAQNIRIPADQEILHVLSREFKVDDQAGIKDPLGMVGVRLD
                     AQVHIVTAPVTQLQNTEKAVEQAGVRVSNKVLSALASSQALLTEAEKDLGVAICDIGS
                     GVIDLIIYVDGGVEYSATICLGGQHVTQDLSIGLKTPIDAAEMLKKRKGTARAADVDP
                     MEMVDVPSVGERAPRQIPRRDLALIIEARMREMLELVDMELMKSGRKNILAGGIIFTG
                     GGAQLEGLVGLAEEVLQLGASVAYPKGLLGISDKVASPPYSTATGLILYQARYGETET
                     QKPGRNLLGKVKSWLQKNL"
     gene            4803..5981
                     /gene="ftsZ"
                     /locus_tag="PLF85_07775"
     CDS             4803..5981
                     /gene="ftsZ"
                     /locus_tag="PLF85_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNE19602.1"
                     /translation="MLAFEEKKKSPATIKVVGVGGGGMNAIERMVSMNLEGVELIAMN
                     TDEQVLERSHAPIKVALGSKLTRGMGAGGIPDVGAQAAMEDREKIMNVLKGADMVFVT
                     AGMGGGTGTGAAPIVAEVARELKALTVGVVTLPFIVEGEKRMSYAKQGQNNLRDRVDT
                     LITIPNEAIFKVIDKKTSVGAAYKLIDDMLYRAVRGISNIINSTGFVNVDFADVRTVM
                     GQNGDAVIGLGEGTGENRVAEALQSAIHHPLLNGKTIDGAQAVLINVVGGSDFALYEY
                     HEIQKQIHEQVSKDAVIIVGFTEDETKDNHVSITVIATGYRRKEAGMPAPEQRTMAQP
                     GQPQKQFQPQILPRTGTEDSTYRTGAGQQGLDFPRVDTSVQGAVYDIPTFLRKMPKVE
                     "
     gene            6046..7860
                     /locus_tag="PLF85_07780"
     CDS             6046..7860
                     /locus_tag="PLF85_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNE19603.1"
                     /translation="MAWPLTFFTFVSFALFSGGYRVFASLAVLATLTFLLYSYRKFGH
                     RFIPVLLVGVLLGTWLFSLREGFRREKEPPAEGIARVSSVSRRSVILTLDDSSKLRLT
                     GFRPEQLPLRGAIVRFSCRLFPIPEGDFAAFERLSGVRTWCEKISVSTITNPRLASLR
                     SAALDFLRRRFATMGEESLIAAFLLGDTETMPAKTLAAFRDMGLMHLFAVSGLNVALL
                     FALLYLPFRFAGIPAVGALLGFLIATAFLLLLDFPVPLFRAWLFLAIGALARLLDRRL
                     SPWTLLFLTALVVETLFPLSTFTISFILSFGITAAILLFYQPIYFCFSREGRIRQLIA
                     GHVALTLAAGLPAFVLTYLLFGSAQPLSVIYNLLLVPFSGLYLFAAILYIPFDAAGYL
                     LQGLDALYLWFAGKHSLYAMRHLPVPDTRALLAPLVAIAAILLALLYWQARKRLWSAR
                     RNLRWGIPTILIVFALPWFITPRVQTAFYAVPDKVWSFGSGRLMLAGKELFAITQPDI
                     CLPVTTEGNGVIRGGLLKLQPNCFAFAASLKPELWQVNEFAQCQSVQLFQSKKRATQA
                     AEWEKLFHLFGLRGPVELRNYFTWYADRPLSCAKSKPL"
     gene            complement(7918..>8609)
                     /locus_tag="PLF85_07785"
     CDS             complement(7918..>8609)
                     /locus_tag="PLF85_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE19604.1"
                     /translation="KGEAKFSLVSRNKELLDALASNLQSPAKVFPCDLGQPQKALETL
                     AQATQQNGPVDLLINNAGMQFVDHFTSISEADAARAMDLNYTTPTALMRSVLPAMASR
                     NSGVVVNIASLAAITPTPYMAEYAASKAALAALSTALTGEYKDTAVHFLTVYPGPVKT
                     PMAEKAQEKYSGSAAQNVPYGTAEELAAEIRKAIQNKKKKLVYPALYRSAEYFRDFAI
                     WMTAEFAPKPK"
BASE COUNT         1828 a         2482 c         2373 g         1926 t
ORIGIN      
        1 gtatcggtca gcaataacaa cttctctgcc cccagcgcag aagcaattgc ccccgccata
       61 gtgtccgcgt tgatgttgag cgagttaccg tcgtcatcaa acgccaccgg agcaatcact
      121 ggtatgaacc caccggcttt cagcgcgttg agcacctgca cattcacatc gcggggctca
      181 accgacccga cgcggccgag atcaatcagc tctgttccct gctcagtctt gtgttcgatt
      241 ttatgccgtg tcgcgcgggc cagtgcaccg tcacgccctg aaatccccac cgctttaccg
      301 cctttgcgtg cgatctgtgc aacgatgcgt ttgttgacat caccgccaag aaccatttca
      361 accgcggcca tttcttcatc gccggtaaca cggtggccct gtatgaattc agattttacc
      421 ccgaggcgcg atagcagctg gttgatcatg gggccgccac cgtgaacaat cacaggatca
      481 ataccacagg ccttcagcat cacaacgtcc tgcgcgaagc ggtcgctgag atcggcctta
      541 tccattgccg ccccgccgta tttgatgacg agtgtcttgc cggcgaagcg ctggatatac
      601 ggcagcgctt ctaccagcgt gccgatacga tcgtgggagt tttcgtttga gcccaagagg
      661 gagttatttc tttttcgcgg cctttttctt tggcgtcgcg gtacgccgcg cagaaggtct
      721 gccgccgcgc gctttaggag caggaatcga ttttggccgg tgcgactctt cgcgctcgtg
      781 cgtgtgcgga gtgatatcgc gctctggcac atgctcctgc gcttcaaccg gggcattgcc
      841 ctggttcata ccacggcgaa actcgtgtat acccgcaccc aggccacgcg cgagttctgg
      901 cagtttcttg ccgccgaaca gcagaagaat gatgaccacg aggaaaatca tttcgcccat
      961 gttgggcata ccgaagagga gaaccatacg tgagccctgc gcggaaagac agcgtgtaaa
     1021 ttgtttttcc tgcagtgaat tcaaacctcg caatgcgcca aagagcacgt ccataaacgc
     1081 tgtacagcac cacgcgcccg aggtattatg tctcatcatg aagctgccgt tcggcctgtt
     1141 tacgtcctgc tgtgtatggg ctgtcgttat acttggcggc gcagccggct gcggcaaaaa
     1201 agccgaacca gtggcggctt caggcagtga actgccaccg gctgcaaagc caggcgatgc
     1261 cccggagttt atccgcagcc tctcgctact ctacccccag gcagaacttt accgggccga
     1321 agcggcagaa aaaaatgtcg gcaaagcggg tatcattttg cagaaaacca accatccgct
     1381 cgaagacgtt gtgcgctact atggcgacac gctgcgaaag cacgggttca ccgagacgac
     1441 tcacctcgtg cagaccaatg gtgctctgct gcagtatgag cgccgcgccg acaagaacgc
     1501 agacaaacgc gagctggtat cggttgacat tagcaaactc ccctacgcag agaatttact
     1561 gatacgtgtc ggccggtctg aaactgatta cagccgcggt ggcaacccat gaaaaagact
     1621 gaaccgcgcg aagtacaact ggatcgcagc tatagctttc tcgactttca ccgtttcttg
     1681 ccctacgtct tcttcggact cttcctgttt ctgctcgggc tcgtcagctt cgtctactac
     1741 agctacattt atgccttctt catctcagga atcctgttca tattgttccg cactccgcac
     1801 cgctggttat tgagcaagct cgacgggcgg cggacgatgg cagcggctat ctcgactgtg
     1861 tttgttatac tgagcctgat tatcccggcg atatttctgg cgatcgcgct cttcaccgaa
     1921 gcgcggcatg cttcagaact ggcgcgcgaa tggttcacgc cggaaaaact ctttgagata
     1981 catcgcaaca acccgtggat agaaggacgg ctcgatatca gcgcgcagca acttctggcc
     2041 taccggctgc agctgctgga tgcgatacgc aacaaccagc tcgaaactct caaacagggc
     2101 tggacgtggg cgacaaccgg cctcaaattc ttcgtcgact tcactttcgc attgtttatc
     2161 ctgtttttcc tgttccggag cgtggacaga atcggcctgg cagtttaccg caacctgccg
     2221 tttccggata aaattgagaa gcacatcggc gaccgcatgg tgcagatatt cgacgccgtg
     2281 gttaaaggta acctggtgat ttcgatggta caggggagtg tcatcggcat cgtcttctgg
     2341 ttgtgcgacc tcagcactcc cctgctctgg ggagcagtgg cagcgccgtt tgcgctgatt
     2401 ccggtcatcg gcacggcagt tgtctggctg cccggtgcac tctacctcta cacgcataac
     2461 caggaaacca ccgccgtggt catgggtatt acctgcctcg tcgcttactt cctgcttgag
     2521 aacctggtca agccgctgct gctggacaaa gatctcaacc tgcacccgtt gtttcttttt
     2581 ctcgcgatcg tgggaggtct gaacgcattt ggcattaagg gcctgatatt aggacctttc
     2641 atcgtgacga tgtttgtcac catctgggaa ctcatcagcg aatggaaccg caacttctct
     2701 gaagccgaag gcggaggcga aacgccgtca gaataggtct acggtcgccc aatcacgaca
     2761 tacatgccgt tacgcaaaaa aatcctctat gccggcctct cgctcttcgc cctgggaggc
     2821 atcttgctca cctggtatgt tgtgaagcgc cattcgggcg acggcgagtg ggctgtcagt
     2881 gtcgagggca accgtatcct gaccgagaaa gacatccagg atgtcgttac ttacctgctg
     2941 cgagccgcaa aagacggcgt cagcgccgac gagatacgcg atgcactgct gctcaatacg
     3001 cgcatcgcca cagcccgggt tgccgtgcta ccaggacagc gcatacagat cgcgatcacc
     3061 gaacgcacgc tcgagtacct gcaaaacgaa ggtaatggcg tcgccgagaa ggacggcaaa
     3121 ggcacgacaa ttgtcgaaaa tgcctcgcag atacaccgcg acttcacgcc agacaaagtc
     3181 attttctact tgacttttgg cggtgaatca atgacttcac caagaagtga cataatccgg
     3241 ctttggaaag acacacaggg aaagtatgca tttctctggc aacgtcttgc cgaaatcgag
     3301 atacagccgc tgaaagaccg cacgacagat gaaacgggta aacccggaat ctggcgctac
     3361 cgaatctatt cagcgggtat tcgctcctgc gtggtgtatg agggccgctt ctctgaagag
     3421 accctgcgcc ggttgtgggc agtgtacgcg tacctcgaag ccaagctgcc gcgatcggtg
     3481 acactggtag acctgcagga aaacagcgca attatacgcg aaatgaaaac gaaccgaagc
     3541 ggcacagagg gcgacacatg agttatgacg cggggtcaca cggcggccgc atcgtcgcag
     3601 cccttgacct gggttcgagt aagacggttg ccattgtagc ccgcgtttca tcgcccgaag
     3661 acattgaaat catcggcctg ggtgtcgtcg aagcacgcgg ggtcaaatcg ggctctgtca
     3721 ccaacatcga actaacagta aaaagcatac gcgacgccat tgaagaggcg gagctcatgt
     3781 cagctgtcga aatcgacgaa gtcattatca atatctccgg caagaacgtc cgcggcgaca
     3841 atgccaacgg catggtaacc attacgggcc gcgaccgcat tatcagccac gcggatgtat
     3901 tccgcgtcat cgagcaggcg cagaatatcc gtattcctgc cgaccaggaa atactgcatg
     3961 tgctctcgcg tgagttcaag gtcgacgacc aggccggcat caaagaccca ctcggcatgg
     4021 ttggtgtccg cctcgacgca caggtgcata tcgtcactgc acctgtcacg caactacaga
     4081 acaccgagaa ggccgttgaa caggctggcg tgcgcgtctc gaacaaggta ctctcggccc
     4141 ttgcctcgag tcaggcactg ctcactgaag ccgagaaaga tctcggtgtg gcgatctgtg
     4201 acatcggctc gggagtcatc gacctcatta tctacgtcga cggcggcgtt gaatactcgg
     4261 cgaccatctg cctgggtggc cagcacgtca cgcaagacct ctcgattgga ctcaagacgc
     4321 caatcgacgc ggcagaaatg ctgaaaaaac gcaagggcac cgcgcgcgcc gcagacgttg
     4381 accccatgga aatggtcgat gttccttctg tgggcgagcg tgcaccacgg cagattccac
     4441 gccgcgacct tgcgctgatc atcgaagcgc gtatgcgcga gatgctcgaa ctggtcgaca
     4501 tggaactcat gaaatccggc aggaagaata ttctggccgg cggtattatt tttacgggag
     4561 gcggcgcgca gctcgaaggc ctggttggcc ttgcagaaga agttctgcag ctgggtgcgt
     4621 ctgtcgccta tccaaagggc ttgttgggga tttcagacaa ggttgcatca cccccgtaca
     4681 gcacggcaac aggacttatc ctgtaccagg cgcggtacgg tgaaacagaa acacaaaaac
     4741 cgggacgcaa cctgttagga aaagtgaaga gttggttaca gaaaaacctg taggagcgca
     4801 cgatgctggc atttgaagaa aaaaagaaat cccccgctac aatcaaggta gtcggcgtgg
     4861 gcggcggcgg tatgaacgcc atcgaacgta tggtttcgat gaacctcgag ggtgttgagc
     4921 tgatcgcaat gaacaccgac gaacaggtgc ttgaacgctc gcatgctcct atcaaggttg
     4981 cgctcggttc aaaactcacg cgcggcatgg gcgctggcgg tattcccgac gtcggtgcgc
     5041 aggcggctat ggaagaccgt gagaagatca tgaacgtgct caagggcgcc gacatggtgt
     5101 ttgtcactgc gggcatgggc ggtggtaccg gcaccggtgc tgcgccgatc gtcgccgagg
     5161 tggcgcgcga actcaaggca ctcactgtcg gtgtcgtcac gctgcctttc atcgttgaag
     5221 gtgaaaagcg tatgagctac gccaagcagg gacagaacaa cctgcgagac cgcgtcgaca
     5281 cgctgatcac tatccccaac gaagcgatct tcaaagttat tgataaaaaa acttcagtcg
     5341 gcgctgccta caaactgatc gacgacatgc tctaccgcgc ggtacgcggt atcagcaaca
     5401 ttattaactc caccggtttt gtgaacgtcg actttgccga cgtgcgcacg gttatgggcc
     5461 aaaacggcga cgccgttatc ggtctcggtg aaggcaccgg tgagaaccgc gtcgcagaag
     5521 cgctgcagag cgcgatacac catccgctat tgaacggcaa gaccatcgac ggcgcgcagg
     5581 cagtactgat caacgtcgtc ggcggttcag acttcgccct gtacgaatac catgagatcc
     5641 agaagcagat ccacgagcag gtatcgaaag atgcagttat catcgttggt ttcactgaag
     5701 atgaaaccaa ggataatcat gtatcgatca ctgtcatcgc gaccggctac cgccgcaaag
     5761 aggcgggaat gcccgctcct gaacaacgga cgatggcgca gccaggccag ccgcagaagc
     5821 aattccagcc acagattctg ccacgcaccg gcacagaaga cagcacatac cgcacgggtg
     5881 caggccagca gggtctcgat ttcccccgtg tagacacttc agtgcagggc gcagtttacg
     5941 acatccccac tttcctgcgc aagatgccca aagtagaata atcgggccgg cggcggcccc
     6001 aatcgtactt tacaggccgg ccggcgcgcc gaacaactcg cggcgatggc ttggccatta
     6061 acatttttca cgttcgtttc gttcgcgctc ttttcgggtg ggtaccgggt tttcgcctcg
     6121 ctcgcagtac ttgcgacact cacgtttctt ctttattctt accgaaaatt cggtcacagg
     6181 tttatccctg tcctgctcgt gggagttctg ctcggtacgt ggctgttttc tctgcgggaa
     6241 ggctttcgca gagagaaaga accaccagcc gagggaatag cgcgcgtcag cagcgtgtca
     6301 cgccgctcgg taattctgac gctcgatgac tcaagcaagc tgcggctgac gggttttcgt
     6361 ccagagcagc tgcctctgcg cggggctata gtaaggtttt cctgccgcct gtttcccatt
     6421 cccgaggggg atttcgcggc tttcgagcgc ctgtcaggcg tgcgcacgtg gtgtgagaag
     6481 attagcgtaa gcacaatcac taacccacga ctcgcttcgc tgcgcagcgc ggcgctcgat
     6541 tttttacgcc ggcggtttgc cactatgggc gaagagagcc tcattgccgc ctttttgctc
     6601 ggcgataccg aaaccatgcc ggcaaaaact ctcgcggcgt ttcgcgacat gggactgatg
     6661 caccttttcg cagtttcagg cctgaacgtc gccctgctct ttgcgctgct ctacctgccc
     6721 tttcgttttg cgggaattcc tgcggtgggt gcgctgctcg gcttcctgat tgcgactgcc
     6781 ttcttactgc tgcttgattt tcccgtgccg ctgttccgcg cctggctgtt tctcgcgatc
     6841 ggggcgcttg cacgcctgct cgaccggcgg ctgagtccat ggaccctgct ttttctcacc
     6901 gcgctggttg tggagacact atttccactg agtaccttca cgatttcgtt cattctgtcg
     6961 ttcggcatca cggcagcgat cctattgttc taccagccca tctatttctg cttcagccgt
     7021 gaagggcgaa ttcgtcaatt gatcgcaggg catgtggcgc tgacccttgc tgcgggtttg
     7081 ccggccttcg tgctcaccta cctgctgttt ggtagtgcgc aaccgctttc ggtgatctat
     7141 aaccttttgc tcgtgccctt ctctggtctc tacctttttg ccgccatact ctatattccg
     7201 ttcgatgccg cgggctacct gttgcagggg ctcgatgcgc tctacctctg gtttgccgga
     7261 aaacacagcc tgtatgccat gcgccatttg cccgtgccag atacgcgcgc attactggcg
     7321 ccgctcgtcg ccattgcagc gatactgctg gcgctgctgt attggcaggc acgcaaaaga
     7381 ttatggagcg caaggcgcaa cctgcgctgg ggaattccta caatcctgat cgtttttgcg
     7441 ctgccctggt tcattactcc ccgtgtacag acggccttct acgcagtacc cgacaaggtg
     7501 tggagcttcg gctcaggtcg cctcatgctt gcgggaaagg agctttttgc cataacccag
     7561 ccggacatct gcctgccggt gaccaccgag ggcaacgggg tgatcagggg tggcttgctc
     7621 aagcttcagc ccaactgttt tgccttcgcc gcttcgctga agcctgaact ctggcaggtc
     7681 aatgagtttg cccagtgtca atcggtgcag ctcttccagt cgaagaaacg cgccacgcag
     7741 gcagcagaat gggaaaaact cttccatctc tttggcctac gcggcccggt cgagctgcgg
     7801 aactacttca cctggtatgc tgaccggccg ctcagttgcg ccaaatcaaa gccgttatag
     7861 gatctccctg gtaagggtag actcaactct accgctacca gttttttttg aacaatttta
     7921 tttcggcttc ggcgcaaact cggcggtcat ccagatcgcg aaatcgcgaa agtactcggc
     7981 gctgcggtag agggcaggat ataccagttt ctttttcttg ttctgtatgg ccttgcgtat
     8041 ttctgcggcg agctcttctg ctgtgccata aggaacattc tgcgccgccg aaccggaata
     8101 tttttcctgc gctttttctg ccatcggggt cttcaccggc ccagggtaca ccgtcagaaa
     8161 atgcaccgcg gtatctttgt attctcctgt cagcgcggtg gaaagtgcag ccagcgccgc
     8221 ctttgatgcg gcatattcgg ccatgtaggg cgtcggcgta attgccgcga ggctcgcgat
     8281 atttaccact acccccgaat tgcggctcgc cattgccggt aacacgctgc gcatcagcgc
     8341 tgtgggagtt gtatagttca gatccatggc tcgcgcggca tcggcttcgc tgatcgaggt
     8401 gaagtggtcg acaaactgca tgccggcatt gttgatcagc agatcgaccg gcccattctg
     8461 ctgcgtcgcc tgagccaggg tctcgagcgc tttctgcggc tgcccgagat cgcagggaaa
     8521 aaccttcgcc ggcgattgca ggtttgacgc gagtgcatcg agcagttctt tattgcgcga
     8581 taccagtgag aactttgcct cgcccttgt
//
LOCUS       DAOQJF010000119         8554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4437_length_8554_cov_4.951524, whole genome shotgun sequence.
ACCESSION   DAOQJF010000119 DAOQJF010000000
VERSION     DAOQJF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4437_length_8554_cov_4.951524"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLF85_07790"
     CDS             complement(<1..1084)
                     /locus_tag="PLF85_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE19605.1"
                     /translation="MTATTGDNIDLSKLDHNLAGAPFPYFQPIISIEDKRVVGYEVLA
                     RGRDVQGNITSLGYLFEKYADSRVAIELDLSIREAAFRQYGQSAVAQGKKLFINVLPR
                     WLNYTIRDDLSDVIDPLIEQTTRYGIKPENVVVEITEGEYLQHPDTMTRAIHELKNYG
                     FEIAVDDFGAGYSNMSRIGQMRPKFIKLDLHLIRKGFSDLVYKEILNSLAFLSEKIGA
                     ILLVEGIEHEEEVYGALDIGARYMQGYFLGFPESSFSNLDELQQILHDYMRDFTARKL
                     THMQSLMYFKSITSEYFDIHLKELFLPEMKEGETHFRPIEPHSFPAFLRDHLRLVYFL
                     NAQGVQISPNYRLVKDDDGFRWQMDHN"
     gene            complement(1261..2220)
                     /locus_tag="PLF85_07795"
     CDS             complement(1261..2220)
                     /locus_tag="PLF85_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNE19606.1"
                     /translation="MHILSRVADSIYWLNRYVERVENYARLLETYQQFSLENPEITAP
                     LWLALVESTADTSGFNETYDELQPENVVEYLTFNRRNPNSIVSCLYAARENARSIREK
                     ISKDMWESINELYNDVVTVDAARVKAGAQSGSHNFYRDIRRSCHAFYGISDGTLNHDE
                     TYFFALLGRAIERADKTTRILDLQSYMLTSDTQQQFGEGQIVLGLALLRATSAHEMFN
                     QAHARITSENILDFLLLSRTFPRAIIACLLQIEYALRCLAGEQKGFFINAAQKKVQRL
                     LLETRHVDVASLVGQALNGYLDNLQVRLNEIGIEITNTYFTAA"
     gene            complement(2229..3680)
                     /locus_tag="PLF85_07800"
     CDS             complement(2229..3680)
                     /locus_tag="PLF85_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNE19607.1"
                     /translation="MLMERYSTVQAFDEMVTKDGGVRPQYESLGNRLQHMGLGELVRR
                     KGKAEKALFSSGITFTLYGEQGSEDRILPFDIIPRIIRADEWSTLEAGLVQRTLALNA
                     FLNDVYGKQHIIKDGVVSREVIESSPGYLPQCADARPAKGIYTNISGTDLVRGEDGTY
                     MVLEDNLRCPSGVSYVLENREVMKQIFPELFANLRIRPVSNYPFRLRQALGYLSDKAN
                     PTIALLTPGVYNSAYFEHSFLAQQMGIPLVENKDLVVESDKVYMKTIRGLKQVDVIYR
                     RTDDTFIDPNAFRKDSLLGVPGIFKAYRKGNVALANAPGTGVADDKVVYAYVPDMIRY
                     YLKEEPLIANVPTYLCFRDSDRQYVLENLDKMVVKAANGAGGYGMLIGPHASGEEREK
                     FREKILTDPRGYIAQPMISLSTAPTIVDNPVLNAGEGTIEPRHIDLRPYIVFGKDCYV
                     LPSGLTRVALKKGSLVVNSSQGGGTKDTWVLDT"
     gene            complement(3754..4632)
                     /locus_tag="PLF85_07805"
     CDS             complement(3754..4632)
                     /locus_tag="PLF85_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE19608.1"
                     /translation="MAEYEVTHTTVYSYSEKVSHCQNIAYLLPQSDARQSCEKTEIAI
                     SPQPTISADHTDYFGNRFRYFSVEQPHQQLSVTAKTRIRTQKYGDGDGENSLPWDILA
                     QNLRNPTEAADIAAVEYLLASPFVALSDTFGEYATESFTPGRPILAAALDFARRIRND
                     FKYEQKSTTILTPLTEVFRHRRGVCQDFAHLAIAALRTIGIPARYVSGYIETYPPEGQ
                     PKLRGSDASHAWISVYAGTGGWFDFDPTNGKPITEEFIVTARGRDFGDVSPLKGILFG
                     GGKHTLKVEVDVHRLT"
     gene            complement(4625..7222)
                     /locus_tag="PLF85_07810"
     CDS             complement(4625..7222)
                     /locus_tag="PLF85_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNE19609.1"
                     /translation="MQRTVTAMAEVPQPLLQAYSALPDTYDELLDASGNPREKYKFLL
                     NSYQEIGEAELELRQRELARLLKENGVTYNVYGNQDEKARLWSLDMMPYLMESTEFSA
                     LERGLLQRGELLNTLFKDLYGPRRLLFDRVVPPEVVFAAQGFLRPCHNLYQNRERELL
                     FFSCDLARRSDGTFVVIGDRSQAPSGTGYALENRIALSRVFPSLYRDSEVHRVAMFFR
                     GMRKALSALPRARENREPVIVLLTPGPENETYFEHVFLAGYLGYTLAQSTDLTVRANR
                     VYLKTVEGHQQVDVVLRRTDDQFMDPLELRGDSLLGVPGILEAVRSGNVSVVNPLGAA
                     VLENRSLMAYFPALCRYYFGEELILPNCETLYLGDATHREQLFSDASRFVVKPVSREG
                     TRRHIYPAQLASAELARLRETILARPHDFIAQEIIPGSSVPIIDQGRRIVAARCVIRT
                     YQVATESGYQVMPGGLARISGNADDLVITNQSGAGSKDIWILASEPQKEVSLLRAETV
                     ESRISRKSSGGVPSRIADNIFWMARYAERAENMSRLLRNVIARITQIEDTAGTEEIAR
                     LLRMVTHISGEYPGFVGDDSAELIRHPEGELGRLIYDRDAAGSVRYNVLSLVRASRSL
                     RDSLSDDMRRVISQLENEPYASGVLSVQHAHLFDVIIYLSSVSGLSQENMSREVGWHF
                     LELGRRIERASFSLRVLRAFLFLGDIYDKFIVENLLNINDIRITYQRRYRHRIETDSV
                     LDILLYDESNPRSLGYQLVRIRDFIPALPAKTEGARPAAEKIALRLYTEYKVTDVREL
                     LGGEKAGDKLARWIDTLESGLTDLGSNLRETYFTYVEEQHSIGDHTRSGILRRAQEMT
                     EGENEVANG"
     gene            complement(7225..>8554)
                     /locus_tag="PLF85_07815"
     CDS             complement(7225..>8554)
                     /locus_tag="PLF85_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE19610.1"
                     /translation="SRLNFFRITPDPGVIEVNMFPSASLVDSLAKTRIIYEEAERTGL
                     TTDRYMLDGRACATGGGNHITLGALHSEDSPFLRKPHLLASLVAYWQNHPSLSYVFSG
                     LFIGPTSQAPRLDEARDGQVAEAEIALTQIRRAETLPPWQIDRILRNILVDITGNTHR
                     AEISIDKLYAPGSYTGRLGLVELRGFEMPPHYQMSVVQQLLVAGLLSWFWREPYRHPL
                     TRWGSSLRDEWMLPFYLERDLMRVLYDLSGAGYAFAFHHVEPFFEFRFPVYGTYRAGG
                     FEIELRMALEPWNVLGEEVVSGSVSRAVDSATERVQVLVRGNLKDTVITCNGYALPLR
                     YDRDQDCHVAGVVFKAWSPPFTLHPTVPVHSPLQFGIYDRRSRQYTGGCSYHTSHPGG
                     RAYEKFPVNSYEAESRRLSRFLPQARYPQVPPIKPMVSDFPHTLDLRREG"
BASE COUNT         1901 a         2402 c         2380 g         1871 t
ORIGIN      
        1 agttgtggtc catctgccag cgaaaaccgt catcgtcttt caccaggcgg taattcgggc
       61 taatctgaac cccctgcgca ttgaggaagt agacaagccg caggtggtcg cgcaaaaaag
      121 ccggaaaact gtgcggctca atgggccgaa agtgcgtttc gccctctttc atctcaggca
      181 aaaaaagctc tttcaggtga atatcgaagt actcagaagt gatcgatttg aagtacatga
      241 gactctgcat gtgcgtgagc ttacgcgcag taaagtcgcg catgtagtcg tgcagaatct
      301 gctgaagctc atcgagattt gagaacgaag attccggaaa tcccaggaaa taaccctgca
      361 tgtagcgcgc cccgatatcg agtgcgccat acacctcttc ttcgtgttcg ataccctcaa
      421 ccagcaggat tgccccaatc ttttccgaga gaaaagccag cgaattgaga atttctttat
      481 agacaaggtc ggaaaacccc ttgcgtatca ggtgcaggtc gagcttgata aacttcggcc
      541 gcatctgccc gatacggctc atgttcgaat aaccggcacc gaaatcgtcc acggcaattt
      601 caaacccgta attcttcagc tcatgaattg cccgggtcat tgtatccggg tgctgcaggt
      661 actcaccctc ggtaatctcg acgacaacat tttcgggttt gataccgtaa cgggtggtct
      721 gttcgatcag cggatcgatg acatcgctca aatcatcgcg tatcgtgtag ttgagccagc
      781 gtggcagcac gttgatgaaa agctttttgc cctgcgcaac tgcagactgg ccatattgcc
      841 tgaaagccgc ctcgcggatg ctcaggtcca gttcaattgc cacgcggctg tctgcgtatt
      901 tctcgaacag gtaccccagg gaggtgatgt taccctgcac gtcgcgcccg cgcgccagta
      961 cttcgtaccc cacgacccgc ttgtcttcaa tcgagatgat tggctgaaaa tagggaaacg
     1021 gggcaccggc caggttgtgg tcaagcttgg aaagatcgat gttatccccc gttgtggcgg
     1081 tcaccaaaga aaactggaaa ttctcctatg ggcgtaaaca ataaacagac agaatttttc
     1141 ccatctgtgc aaagcaccat gcggcggccc aaatgagtgc caaaaaaagt tgactgaaac
     1201 cgaccgcgct ccatttccgc acccccgttc tggaaattcc tgcagccctg ttgcaccggg
     1261 ttaagctgca gtaaaatagg tattggtgat ctcaatgcct atttcattga gccgcacctg
     1321 caggttgtcg agatatccat tgagtgcctg gccaacgagc gatgcgacat cgacatggcg
     1381 tgtctcgagc agaagacgct gcaccttttt ctgtgccgca ttgatgaaaa agcccttttg
     1441 ttcgcccgcg agacaacgca gtgcatattc aatctgtaac aggcaggcga tgatagcacg
     1501 gggaaaagtg cggctcagca gcagaaaatc gagaatgttc tctgaggtaa tacgagcgtg
     1561 tgcctggtta aacatctcat gggcgcttgt cgcacgcagc aaggcaagcc ccaggacaat
     1621 ctgtccctcg ccaaactgtt gctgcgtatc gctcgtcagc atatacgact gcagatcgag
     1681 aatgcgtgta gtcttgtctg cacgctcgat ggccctaccc agcagcgcga agaagtaggt
     1741 ttcgtcgtgg ttgagcgtac cgtcggatat tccgtagaac gcatggcagc tgcgccggat
     1801 gtcccggtaa aagttgtggc ttcccgattg agccccggct ttcacgcgtg ctgcgtcaac
     1861 ggtcaccaca tcgttgtaga gttcgttaat cgactcccac atgtccttgg aaattttttc
     1921 gcgtatcgag cgggcattct cgcgcgccgc gtacaggcag gagacaattg aattggggtt
     1981 tctgcggtta aaggtgagat actcgacaac attttcgggt tgcagttcat cgtatgtttc
     2041 attaaaaccg gaagtgtctg ctgtagattc gacaagtgcc agccacaatg gcgcagtaat
     2101 ttccgggttt tcgagtgaga actgctggta agtttccagc agccgggcat agttttccac
     2161 gcgctcgacg tagcggttca gccaatagat cgaatctgct acgcggctga gaatgtgcat
     2221 atcttgcctc aggtgtcgag cacccaggta tccttggtgc ctccgccctg cgaagaattc
     2281 acgacgagcg accctttctt cagcgcgacg cgcgtcaggc cgctgggcag aacgtaacaa
     2341 tcttttccga agacaatata tggccgcagg tcaatatgcc gcggctcgat cgttccttcg
     2401 cctgcattca gtaccgggtt gtccacgatc gtcggcgcgg tcgacagtga tatcatcggc
     2461 tgcgcaatat acccccgcgg gtcggtgagt atcttttcgc ggaatttctc gcgttcttct
     2521 ccgctggcat gcgggccgat cagcatacca taaccaccgg caccattggc agccttcacc
     2581 accattttgt cgaggttctc cagaacatac tggcgatcgg agtccctgaa gcagagatag
     2641 gtagggacgt tggcaatcag cggttcttcc tttaaatagt agcgtatcat gtcgggaacg
     2701 taagcgtaca ccaccttatc atccgccacg cctgtacccg gcgcgtttgc cagcgccacg
     2761 ttgcctttgc ggtatgcctt gaagatgcct ggaaccccga gcaacgaatc tttgcggaac
     2821 gcattcggat caataaacgt atcgtcggtc cgccggtaga tcacatccac ctgcttaagc
     2881 ccgcggatcg tcttcatgta gaccttgtca gactcaacca cgaggtcttt gttttcgacg
     2941 agaggaattc ccatctgctg tgccaggaac gagtgttcga agtatgccga gttgtatacg
     3001 ccgggggtca gcagcgcaat cgtgggattc gccttgtcac tcaggtaccc cagcgcctgc
     3061 cgtaaccgga acgggtagtt ggaaaccggc cgtatgcgca ggttagcaaa gagctctggg
     3121 aagatctgct tcatcacctc gcggttttcg agcacataac tcacgcccga aggacacctc
     3181 aggttgtctt cgagcaccat gtacgtgccg tcttcgccac gcaccaggtc agtgcccgaa
     3241 atattggtat agataccttt cgccggccgg gcatccgcgc actgcggcag gtaacccggg
     3301 ctcgactcga tcacttcacg gctgactaca ccgtctttaa taatatgctg cttgccatag
     3361 acgtcattca ggaatgcatt cagtgcaagc gtccgctgga caaggcccgc ctcgagggta
     3421 gaccattcat cggcgcggat aatacgcggg ataatgtcga acggcagaat acggtcctcc
     3481 gacccctgct cgccatagag cgtgaaggta attcccgagg agaacagtgc tttctccgcc
     3541 ttgcctttac gccgcaccag ctcacccaac cccatgtgct gcaggcggtt gcccaggctt
     3601 tcgtactgcg gccgaacacc cccgtccttc gtgaccattt catcgaatgc ctgcaccgtg
     3661 ctgtatcttt ccattaacat aactgctctg ccttgcggct gcgcgtaagg tgcatgccgc
     3721 aaccatcgtt aagcaaatac catgccagac gtttcaggtc aggcggtgca catcgacttc
     3781 gaccttgagt gtgtgtttac cgccgccaaa aagaatacct ttgagcggag aaacgtcgcc
     3841 gaaatcgcgt ccgcgggctg tgacaataaa ttcctctgtg atcggcttac cgttcgttgg
     3901 gtcaaaatca aaccagccgc ctgtgccggc atagactgaa atccacgcgt ggctggcatc
     3961 gctgccgcga agcttaggtt gaccttccgg tggatacgtt tcgatataac cactgacata
     4021 gcgcgcagga atgccgatcg tgcgtagcgc agcaatcgcc aggtgcgcga aatcctggca
     4081 gacaccgcgg cggtggcgga aaacctcggt gagcggcgtc agtatggttg tcgacttctg
     4141 ctcatacttg aagtcattgc gaattcgccg cgcaaaatca agcgcggcag ccagtatcgg
     4201 gcggccaggc gtaaaagatt cagtggcgta ttccccgaat gtatccgaca aggcaacgaa
     4261 cggcgacgcc aacaggtact ccacggcggc aatatccgca gcttcagtgg gatttcgcaa
     4321 gttctgcgcg agaatatccc acggtagaga attttctcca tcaccgtccc catacttctg
     4381 cgtgcggatg cgggttttgg cagtcacgga gagttgttgg tgcggctgtt caacagaaaa
     4441 gtagcggaaa cggttaccaa agtagtccgt gtggtcggcg cttattgtcg gctgtggcga
     4501 gatggcgatt tcagtttttt cacagctctg ccgcgcgtcg ctctgcggca gcaggtacgc
     4561 aatgttctgg caatggctga ccttttcgct gtagctatat accgtggtgt gcgtgacctc
     4621 gtactcagcc atttgcgacc tcgttttctc cttcggtcat ctcctgcgcc ctgcgcaaaa
     4681 tgcctgaccg ggtatgatcg ccaatgctgt gctgctcttc gacgtaagta aagtaggtct
     4741 cgcgcaggtt gctgccgagg tcggttaagc ctgactcgag ggtatcgatc cagcgtgcga
     4801 gcttgtcccc tgctttttcc ccacccagca gctcgcgcac atcagtgacc ttgtattcag
     4861 tataaagccg cagcgcgatc ttttccgcgg ccggtctcgc gccttctgtt ttcgccggca
     4921 gcgccggaat aaaatcgcgt atgcgtacca gctggtaccc cagagaacgc gggttcgatt
     4981 catcatagag taaaatgtcg aggaccgaat cggtttcgat acggtgacgg tagcgcctct
     5041 ggtacgtgat acgtatatca ttgatattca gcaggttctc gacgatgaac ttgtcgtaaa
     5101 tatcaccgag aaaaaggaac gcgcgtaaaa cacgcagcga aaaactggcg cgttcaatac
     5161 gcctgccgag ctcgagaaag tgccagccca cttcgcggct catgttctcc tggctgagac
     5221 cagagactga agacaggtag ataataacat caaacaggtg cgcgtgctgc accgacagaa
     5281 cacccgatgc ataaggttca ttctcgagct gcgaaatgac gcggcgcatg tcgtcagaca
     5341 agctgtctcg caggctgcgc gatgcgcgta ccagtgacag cacattgtaa cgaacgctgc
     5401 cggcggcgtc gcggtcgtag atgagacgcc cgagttcacc ctcgggatgt ctgattaatt
     5461 ctgcactgtc gtcgccgaca aaacccgggt attctcccga gatgtgcgtc accatccgca
     5521 aaagccgtgc aatctcttca gtgccagcag tgtcttcaat ctgtgtaata cgtgcaatca
     5581 cgttgcgcag caggcgagac atattttcgg cacgttcagc atatcgtgcc atccagaaga
     5641 tattgtcggc gatacggctc ggcacgccac cgctcgattt gcgcgaaatg cgcgattcta
     5701 cggtctccgc cctgagcaaa gaaacttctt tctgcggttc cgaggcgagt atccagatat
     5761 ccttgctgcc tgcaccgctc tggttcgtga tgaccaggtc atcggcattg cctgatatgc
     5821 gggccagccc cccgggcatg acctggtaac ccgattcggt ggccacctga taggtacgga
     5881 taacgcacct tgcagcaact atccgcctgc cctggtcaat gatgggcacg gatgaaccgg
     5941 ggataatttc ctgcgcgatg aaatcgtgcg gccgtgcgag gattgtctcg cgcaaacggg
     6001 caagctccgc actcgcaagc tgagccgggt aaatgtggcg ccgggtacct tcgcgcgata
     6061 caggcttcac cacgaagcgt gatgcatcgc tgaacaactg ctcgcggtga gtggcgtctc
     6121 ccaggtaaag cgtctcgcaa ttcggtaaaa tcagctcttc gccaaagtag tagcggcaga
     6181 gcgccggaaa atacgccatc agcgaccggt tctccagcac agctgcaccg agcggattga
     6241 cgacggacac atttcctgaa cgcacggctt cgagaattcc cggtactccc agcagtgaat
     6301 cgccgcgcaa ttccagcgga tccatgaact ggtcgtccgt gcgccgcagc accacgtcga
     6361 cctgctggtg gccttcgacg gtcttgaggt acacgcggtt cgcacgcacc gtcaggtcgg
     6421 tggactgcgc gagggtataa ccaaggtagc cagcgagaaa gacgtgttca aagtaagttt
     6481 cattttccgg accgggtgtg agtaacacga tcacaggctc ccggttttcg cgcgcgcgcg
     6541 gcagcgccga gagcgccttg cgcatgccgc gaaaaaacat ggctacgcgg tgtacctcgc
     6601 tgtcgcggta gaggctcggg aatacccgcg agagcgctat ccggttctcg agcgcatagc
     6661 cggtgccaga cggcgcctgc gagcggtcgc cgatgacgac aaaagttccg tctgagcggc
     6721 gcgccaggtc gcaggaaaaa aataacaact ctcgttcgcg gttctggtac aggttgtggc
     6781 atggacgcag aaacccctgc gctgcaaaga ccacctctgg cggcacaacg cggtcgaaca
     6841 acaggcggcg cgggccgtac aggtctttaa aaagcgtgtt gagaagttcg ccgcgctgca
     6901 acaaaccgcg ctcgagcgcg ctgaactcgg tgctctccat caggtaaggc atcatgtcga
     6961 gcgaccagag ccttgccttt tcgtcctggt tgccgtagac gttataggtg acgccgtttt
     7021 ctttcaacaa ccgcgccagt tcgcgctggc gcagctccag ctcagcttca ccaatttcct
     7081 ggtaggagtt cagaagaaac ttgtactttt cccgcggatt gccggacgca tcgagcagtt
     7141 cgtcgtacgt atcaggtagt gcagaataag cttgcagcag gggttgaggt acttcagcca
     7201 tcgcggttac ggtgcgctgc atgtctaacc ctcacgcctc aggtcaagcg tatgcggaaa
     7261 atcgctgacc atgggcttga tgggcggcac ctgcgggtaa cgcgcctggg gcaagaacct
     7321 ggagaggcga cggctttcgg cttcgtacga attcaccggg aatttttcgt acgcgcggcc
     7381 gccggggtgc gatgtatggt agctgcagcc gccggtatat tgtcggctgc gccggtcgta
     7441 gataccgaat tgcagcggcg aatgcaccgg taccgttggg tgcagtgtga acggtggcga
     7501 ccacgcctta aataccacac cggccacatg gcaatcctgg tcgcggtcgt acctcagtgg
     7561 cagggcataa ccgttacagg taatcaccgt atctttgagg ttaccccgga ccagaacctg
     7621 cacgcgctca gtcgcgctgt cgactgcgcg cgagacagaa ccggatacca cctcttcgcc
     7681 gagcacattc cagggctcaa gcgccatgcg cagttcgatc tcaaaacccc cggcacggta
     7741 agtgccatac acgggaaagc gaaactcaaa gaacggttca acatgatgga aagcaaaggc
     7801 ataaccggcg ccggaaagat cgtacagaac ccgcatcagg tcacgctcga ggtaaaatgg
     7861 cagcatccat tcatcgcgca ggctgctgcc ccagcgcgtc agcgggtgac ggtacggttc
     7921 acgccagaac caggacaaga ggcctgctac cagcagctgc tgaactacgc tcatctggta
     7981 gtgcggcggc atttcaaagc cgcgcagctc cacgagaccc aacctgcctg tataagaacc
     8041 cggtgcataa agtttgtcga tcgaaatctc tgcgcggtgc gtgttgcccg tgatgtcgac
     8101 gagtatgttg cgcagaatgc ggtcaatctg ccaggggggc aacgtttcgg cccgcctgat
     8161 ctgcgtgagc gcaatttcgg cctcggcgac ctgcccgtcg cgggcctcgt ccaggcgtgg
     8221 cgcctgcgaa gtcggtccga tgaagagacc actgaagaca taggataacg atgggtgatt
     8281 ctgccaatag gcaacgagag aggcaagcag gtggggtttc cgcaggaaag gactgtcttc
     8341 agaatgcagt gcgccaagag taatgtggtt accgccgcct gtcgcacagg cccggccgtc
     8401 gagcatgtaa cgatcggtcg ttagccccgt gcgttctgct tcttcataga tgatgcgggt
     8461 cttggcaagc gagtcgacga ggctcgcaga cgggaacatg tttacttcga tgacacccgg
     8521 gtcgggggtg atccggaaaa aatttaaccg gctg
//
LOCUS       DAOQJF010000120         8548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4448_length_8548_cov_4.912516, whole genome shotgun sequence.
ACCESSION   DAOQJF010000120 DAOQJF010000000
VERSION     DAOQJF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4448_length_8548_cov_4.912516"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2590
                     /locus_tag="PLF85_07820"
     CDS             <1..2590
                     /locus_tag="PLF85_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNE19611.1"
                     /translation="DLAGRTAAAVTTTGVTFDNVAPVISGVAPVDSAFINTAAVSYTL
                     SENCASASITWTRTGGSTDGASPHAKALVGAELNAGTKTNIIIANNPTLVDGAVYSVA
                     FACSDAAGNNAAPVTRTNITFDPSAVVISGVTPTSSSFRNTTDVAYNFSEACYSGTVT
                     WTRTGGATDGASPHVVTLVGAELDPGPHGPAPLANNPALVDGAIYSVQFDCTDFAANP
                     STPIIATNVTYDFTAPTISATAPSTGAFRNNNQVSYTYSENCASATITWTRTGGTSDA
                     SSPHVQSVTGADLNAGAHNGIIIANNPTLADGGIYTIAFNCSDTAGNAATTVTNTNIT
                     YDYTAPTVSIQNLRNNSTVHTGHVIGPATDNVAVTQVQFNLDSTTWNNATYTAPDWKF
                     ALPMGASTWRDRTAHTIQVLATDAAGNVTMTPVINVRKGNNRDVNGDGYEDFAVSGYM
                     HDGNQGALYVYYGVAGGLPDDAAGIAPTVQSTTAGDYFGRNSAMADFNGDGFADVAIA
                     APGTGASMPGRAVVHHGSPAGLNASATTTINGVSNLDLFGTSIIAGDMDNDGFPDLAI
                     GAMGYNANTQVGRVYLYRSTGTAISNTVWKTLTGEGLAHQFGSSLAMGDFNQDNSVDL
                     AVAAQRYNSGTLDGRVYVFNTLTALGANTTATPGNALTTGTGGEYMGTSLASGDFNGD
                     GITDLAIGAVYYGANAGRVYVHYGAAGTGLNTTAGGVITGTANPGYFGTFVSVRDMNN
                     DSYDDLISGAHGNGVNAGQGYIFYGQVANFGSLMTNSGGVYSITGENATSFFARGGNG
                     TDVNGDGYADLILGAPMFNASRGKAYVIYGSAAGPLEISAGATANTLLDGQSGQTAEF
                     AISLNR"
     gene            2905..3546
                     /locus_tag="PLF85_07825"
     CDS             2905..3546
                     /locus_tag="PLF85_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19612.1"
                     /translation="MKFMAFCALTLFAVSATFPQDSEPAPAPGFPDNASSIKIRVYKL
                     ALSTSAECTSPTTVFESASGVEADLVSKPTFGKGKIPDGTYRCMMIELAKNINITGAS
                     VCTTPTNKVICNDTQQSQTIGGTTANVTCSGDTGNAQRVTLYFTTQSSNSDVDTYQTR
                     VLLPPLNGTDSTSGIKLDAPLVYPTTKRGVLRIKKTILNSSCAISPKFSISTP"
     gene            3822..4658
                     /gene="lpxA"
                     /locus_tag="PLF85_07830"
     CDS             3822..4658
                     /gene="lpxA"
                     /locus_tag="PLF85_07830"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNE19613.1"
                     /translation="MPKIHPTAIVENPANLADDVVVGPQAYIGPDVKIGPGCVIGIKA
                     TLVNRVTLGKNNQLFTGATIGENGQDLHFNNPEAEVVIGDNNVFREQITVHQPSVKGN
                     KTTIGNNCYFMADAHVAHDCHVGNNVIMVNKTGIAGHVELGDNVLISGIAMVHQFARV
                     GAFAVLGGVSKATRDILPFTLTNGNPALAFGLNVVGLKRSGMSPAERSAIKQAFQVFY
                     LKGLSTSNAIDTIANELLPSLPAGSPEYKRIEYLAGWLQKSKRGFTFHSSKRKEAESE
                     AE"
     gene            complement(4680..5321)
                     /locus_tag="PLF85_07835"
     CDS             complement(4680..5321)
                     /locus_tag="PLF85_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19614.1"
                     /translation="MAGEIRTLAIILRRRATGDESLLLETLCETGALQAFRLPGILKS
                     RKRSAFYYAPGALCEVLYHSQAGHAIVPRNLELVFSPFSERQEYFRLNTVAEILKASE
                     IFEPGEDAVAVFSLLRAFIENLPDSAEASERHANRFYWELLKLMGLAHEPEQDFAAYD
                     MTHGFLTAREVSEMPDSGFRLPCWWLIGSERPQNDRSDSATYRETIRRFLSGR"
     gene            complement(5321..6145)
                     /locus_tag="PLF85_07840"
     CDS             complement(5321..6145)
                     /locus_tag="PLF85_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNE19615.1"
                     /translation="MARRRKITTTIRRVFGRRKSLREKLIQFFRANKITARNEQMQMI
                     LEIIELHEKRAADVKTAEVNLVSIPVDASLKEIETVFRKTGHSRLPVFEDRDGLRHYP
                     GVLFVKDLVSLTTSQRKNFDLQSHLRKPLVVPESQSILSLLREMRLNRHHLALTVNEH
                     GDVTGMITLEDILEEIVGEIQDEYDSVSKQVMQIEPKVYRVDARMSLSDLNDELALNL
                     PEEKFHSVAGFVLHSLAGELKEKASFAYGNARFTLLKFKHRQVKSIVIDLSGRNIA"
     gene            complement(6145..6657)
                     /gene="ybeY"
                     /locus_tag="PLF85_07845"
     CDS             complement(6145..6657)
                     /gene="ybeY"
                     /locus_tag="PLF85_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNE19616.1"
                     /translation="MISLRLLSKKPPQRFATQVRTFARLLPAIQTAVASARWRQKPRG
                     LKRGVTFDIQLVTDAQIAKLNASYRGKQGTTDVLSFSYIEGAIPVFEHETAGEIYISL
                     PQAARQAKAEKHSLSDELAVLVMHGALHLMGYDHEKNTEEAGRMHRLEAKLLGTLAAH
                     GLRAKGLIAR"
     gene            complement(6654..7022)
                     /locus_tag="PLF85_07850"
     CDS             complement(6654..7022)
                     /locus_tag="PLF85_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE19617.1"
                     /translation="MGLKNLLQLRTETEKIGNRSVNLLIFSGKITSDNIFDLNSKVKA
                     IFENGVFDCILDISQLEYINSTGIALLLTIARTVEQNQGKMFLTRPTQFVQELFDMTD
                     LSGRFAIVADLNTARDQFRN"
     gene            7143..7358
                     /locus_tag="PLF85_07855"
     CDS             7143..7358
                     /locus_tag="PLF85_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19618.1"
                     /translation="MESSGKKTPLIDKLLILGGAVTFIGLTVTFSPMLWMATLLALLP
                     VLVMVVLITPTLHLNIAEKQTEDNRAK"
     gene            7389..8501
                     /gene="ribB"
                     /locus_tag="PLF85_07860"
     CDS             7389..8501
                     /gene="ribB"
                     /locus_tag="PLF85_07860"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNE19619.1"
                     /translation="MLKKLKTLPLLSRSKSGIVDVPTALAAFRAGKMLIVTDSEDREN
                     EGDLVLSATDTTPEAVNFMAKYGRGLICVPVTTSRAKELNLQPMVRRNEDVRRTAFTV
                     SVDAKEKTSTGISAHDRFFTIQKLADPASKARDFMRPGHIFPLVARDGGVLVRAGHTE
                     AAVDLCRLTGKAPVGVICEIMNSDGTMARMPDLMKFAREHKLPILTIEALIEYRRKSE
                     QTVTLLQKGKLKTRWGEFTACYYNSSTDDNVHVALVLGKVDGNTPVTVRVHREDVFAD
                     VFHSPTRKNYLPIDDAMAALLKSKKGVFIFLGQKTSQIFGSESSDGDSGLRQYGIGAQ
                     ILNQLGVKKIRLLTRNPRKVVGLEAFGLEITETVSR"
BASE COUNT         2003 a         2390 c         2289 g         1866 t
ORIGIN      
        1 agaccttgca ggccgtaccg ccgcagccgt gacgaccacg ggcgttacct tcgataacgt
       61 ggcaccggtc atcagtggtg tagcaccggt cgacagcgct ttcatcaata ctgcggcagt
      121 ctcgtatacg ctgagcgaaa actgcgccag tgcatcgatt acatggacgc gcacgggtgg
      181 cagtactgac ggcgcctcac cgcatgcgaa agcactggtg ggggcggagc tcaatgcggg
      241 aacaaagacc aatatcatca ttgcgaataa cccgacgctg gtcgacggcg cagtgtattc
      301 ggttgctttt gcgtgcagcg atgccgccgg taataacgcc gcgcctgtga cgcgcaccaa
      361 catcacgttt gaccccagcg cggtggtcat atcaggggtg actcctacca gtagctcgtt
      421 tcgcaatacg accgatgtcg cttacaattt ttccgaagcc tgctattcgg gcacggtcac
      481 ctggacgcgc acgggtggcg ccacagatgg cgcgtcacct catgtggtca cgctcgtcgg
      541 tgccgaactc gatccaggac cacacggccc ggcgccgctc gcaaataacc cggcgctcgt
      601 cgacggagcg atttacagcg tgcagtttga ttgcaccgat tttgccgcca acccttcgac
      661 accgattatc gccaccaatg tcacctatga ctttactgca ccgacaattt ccgctaccgc
      721 gccttcgacg ggcgcgttcc gaaacaacaa ccaggtcagc tatacttatt ccgaaaactg
      781 tgcttcggcg acgatcacat ggacacgcac gggtggtacc tctgatgcca gttcaccgca
      841 tgtgcagtcg gtcaccgggg cagacctcaa tgctggcgca cacaacggca tcatcatcgc
      901 caataaccca acactggcag acggagggat ctacacaatc gctttcaact gcagcgatac
      961 ggcgggcaat gcagccacga ctgtaacgaa cacgaacatt acctacgact acacggcgcc
     1021 gacagtcagc atccagaacc tgcgcaacaa cagcaccgtg cataccggcc acgtgatcgg
     1081 ccccgccaca gacaacgtag cggtaacgca ggttcagttc aacctcgaca gtactacctg
     1141 gaacaacgcg acctacaccg cacccgactg gaagtttgca ttgccgatgg gcgcctcaac
     1201 atggcgcgat cgcacagcgc acaccataca ggtgctcgca accgacgcag cgggtaacgt
     1261 cactatgaca ccagtgatca acgtgcgcaa ggggaacaac cgcgatgtga atggtgatgg
     1321 gtatgaagat tttgccgtaa gcggatatat gcacgacggt aaccagggcg cactttacgt
     1381 atactatggc gtggctggtg gcttgccaga cgatgccgcc ggcattgcac caacagtcca
     1441 gagtacgaca gcgggcgatt actttggtcg caactctgca atggcggact ttaacggaga
     1501 cggatttgct gatgtagcaa tcgctgcccc tgggaccggc gcctccatgc caggacgcgc
     1561 agttgtacac catggatcac ccgcaggtct gaacgcatcg gcaaccacga caatcaatgg
     1621 tgtttcaaac cttgacttat ttggaactag catcatcgcc ggtgatatgg ataatgatgg
     1681 ttttccagat ctggcaattg gcgccatggg atacaatgcg aatacacagg ttggccgggt
     1741 ttatctttat agaagcacag gtactgcgat atcaaatact gtctggaaaa cactcaccgg
     1801 tgaaggtttg gcacatcaat ttggctcatc ccttgccatg ggtgatttca atcaggacaa
     1861 ctcggtcgat ctagccgtag ctgcacaacg ctacaacagc ggaacgctcg acggacgcgt
     1921 ttatgtattt aacactctca cggcattagg ggcaaacacc acagctacgc ccggcaatgc
     1981 actgacgaca ggcacggggg gcgaatatat gggaacctca ttggccagcg gtgacttcaa
     2041 tggggacggt attaccgatc tggccatcgg tgccgtctat tacggagcaa atgccgggcg
     2101 ggtatacgtc cactacggag ctgccgggac aggcctgaac acaactgcgg gcggggtaat
     2161 aacgggtacg gctaacccag gatattttgg cacatttgta tccgtgcgcg acatgaacaa
     2221 cgatagttat gacgacctga tttcgggcgc gcacggtaat ggagttaacg caggtcaggg
     2281 gtatattttc tatggtcagg tagcaaattt tggcagcttg atgacaaatt ccggcggagt
     2341 ctactcaatt acgggtgaaa acgcaacgag cttttttgct cgcggaggta acggcactga
     2401 tgtgaacggc gatggttacg cagatttgat tctcggagca cctatgttca atgcctcgag
     2461 aggcaaagca tatgtgattt acggcagtgc cgcgggcccg ttggagatta gtgctggtgc
     2521 aactgcaaat acgttgttgg atgggcagtc tggccaaacg gcagaatttg ccatatctct
     2581 aaaccgataa gaacacgcga gaaaccagtt tacattaaga gtgaattgaa taggtaaacg
     2641 ttcgttctct tgtggattgg tcacatacta ccgaaacgat caaaaaacag tttacagtca
     2701 ataactacag aggggcatca tcaaaccatc taagtaccga acaatctacg ggagtctaca
     2761 tatgaagaga aactttttga tgcttaccct ttcttgctta gcgcttctaa tggtcgcata
     2821 cacattgacc ggttgcgctg ggagcacaga gagtgcagcc caaactacgg aagaggcgca
     2881 gtttgagctt acaggagatg ctctatgaaa ttcatggcat tttgcgcgct gactctcttt
     2941 gcagtatccg cgacttttcc ccaggattca gaacctgcac cggcaccggg ttttcccgat
     3001 aacgccagct caatcaagat acgagtttac aaactggcat tatctacttc ggccgagtgc
     3061 acctcaccca ccaccgtctt tgaaagcgct agtggcgttg aagcagacct ggtttcaaaa
     3121 cccacattcg gaaagggtaa gattcctgat ggcacatacc gctgcatgat gattgaattg
     3181 gcaaagaata tcaatattac cggtgcttca gtttgtacta caccgacaaa caaggtcata
     3241 tgcaatgata cccagcagag ccaaacaatt ggggggacaa cggcgaatgt tacctgttcc
     3301 ggcgacactg gaaatgcgca acgagtaacg ctctatttca ccacacagtc ttcgaactcc
     3361 gatgtggata cttatcaaac cagagtcctg ttaccccctt tgaacggaac ggattctact
     3421 tccggtatca agttagatgc accgctcgta tatcctacta ctaagcgcgg cgttttgaga
     3481 atcaagaaga ctattctgaa ctcgagctgc gcaattagcc caaaattcag tatttccact
     3541 ccttaagcca aaagaaaccc ggcgcttcgc gccgggtttc ttttggccat aacatccttg
     3601 tcgccgcaaa ccacggttca tccttgaacc atggtccgcg cctctgtcat ccttgacggc
     3661 gcggaccacc tccggagacc gctacgcggt cacggtggat ccctgcacca tgcgccggcg
     3721 gccttaacct ccttgtcggc gcgggccccg atccctccgt ggatcatggt ttgcagcctt
     3781 cgtcatcctt gacggcgcgg acctcagtgc gtccttgcac catgcccaag atccacccca
     3841 ccgctattgt tgaaaatccc gcgaacctgg ctgatgacgt cgttgtcggg ccacaggcat
     3901 acatcggccc cgatgtgaaa ataggcccag gctgtgtcat tggtataaag gcgacattgg
     3961 tcaaccgcgt cacccttggt aagaacaacc agctcttcac gggcgcgacg atcggcgaga
     4021 acggccagga cctgcatttc aataaccccg aggccgaggt cgtgatcggc gacaacaatg
     4081 tgtttcgcga gcagattacg gtccaccagc cgtcggttaa gggcaacaag acgaccatcg
     4141 gcaataactg ctacttcatg gcagacgcgc acgtcgcaca tgactgccac gttggcaaca
     4201 acgtcattat ggtgaacaag accggtatcg ccgggcacgt cgaactcggc gacaacgtgc
     4261 tgatttcggg catcgcgatg gtacaccagt ttgcgcgcgt gggagcattt gccgtactgg
     4321 gcggcgtcag caaggcgacg cgcgatattc tccccttcac cctgaccaat ggaaatcctg
     4381 cgcttgcctt tggccttaac gtcgtggggc tcaagcgttc gggcatgtca cctgccgaac
     4441 gttcagcaat caagcaggca ttccaggttt tttatctcaa gggcctgtcg acctcgaatg
     4501 caattgatac aatcgcgaac gagcttctgc cttcgctacc ggcgggttct cccgaataca
     4561 agcgcatcga atacctcgca ggctggctgc aaaaatcaaa gcggggcttc acctttcatt
     4621 catcaaaaag aaaagaagcg gaaagcgaag cagagtagcc tggccctacc gggctcctgc
     4681 taacggccgc tgagaaagcg gcggattgtc tcgcggtacg tggcagaatc gctgcggtca
     4741 ttctgtggac gctcgctgcc aataagccac caacatggca gacgaaaacc cgaatcgggc
     4801 atctcagaaa cctctcgcgc ggttaagaat ccgtgcgtca tgtcataggc ggcaaaatcc
     4861 tgctccggct cgtgcgccag gcccatcagt tttagaagtt cccagtaaaa gcggttagca
     4921 tgccgctctg acgcttctgc gctgtcgggc agattttcga taaatgcccg caagagactg
     4981 aagacagcca ctgcatcttc acccggttca aaaatttccg aggctttcag gatttccgcc
     5041 acggtattca ggcgaaagta ttcctggcgc tcgctgaagg gcgaaaagac cagctccagg
     5101 ttgcgcggta caatcgcgtg gccggcctgc gaatggtaga gcacctcgca caaggccccg
     5161 ggggcataat aaaaggcgga gcgtttgcgt gacttgagaa ttcccggcag gcgaaacgcc
     5221 tgcagtgcac cggtttcgca aagcgtttct aaaagcagcg attcgtcgcc ggttgcgcgg
     5281 cggcgcagaa taatcgccag agtgcgtatt tcgcccgcca tcaggcgata ttgcgaccgc
     5341 tcaggtcgat cacgatactt ttgacctggc ggtgtttgaa cttgagcagc gtgaagcgtg
     5401 cattgccgta ggcaaaactg gctttctctt tcagctcacc cgcaagcgaa tgcagcacaa
     5461 accccgccac tgaatggaat ttctcttcgg gcaggttgag cgcgagttcg tcgttaagat
     5521 cggagagcga catacgcgca tcgacgcggt aaactttcgg ctcgatctgc atgacctgct
     5581 tgctgacgct gtcatattcg tcctggatct cgccgacgat ttcttcaaga atatcttcga
     5641 gggtgatcat gcccgtgacg tcgccgtgct cgttgacggt cagcgccagg tggtggcggt
     5701 tcaggcgcat ctcgcgcaaa agcgacagta tactctgcga ttcgggtacc accagcggct
     5761 tgcgcaggtg cgactgcagg tcaaaattct tgcgctgcga cgtggtgagg ctcaccaggt
     5821 ctttgacgaa cagcacaccc ggataatgcc tgaggccgtc gcggtcttca aaaaccggca
     5881 gtcgtgaatg tcccgtcttg cggaacacag tctcgatttc cttgagtgag gcatcgacag
     5941 gaatactgac gagattcact tcagcggttt tgacatccgc cgcgcgtttt tcgtgtagct
     6001 cgatgatctc gagtatcatc tgcatctgct cgttgcgcgc ggttatctta ttcgcacgaa
     6061 agaactggat gagtttttcg cgtaacgatt tgcgccgccc gaagacccgt cgtatggtgg
     6121 tggtaatttt tctgcgtctt gccattatcg tgctatcagg ccttttgccc tgaggccatg
     6181 cgcggccagt gtaccgagca acttcgcttc gaggcggtgc atgcgcccgg cttcttctgt
     6241 attcttctca tggtcgtacc ccatgaggtg cagagcaccg tgcatcacga gcacagcaag
     6301 ttcgtccgac agggaatgtt tttctgcttt tgcctgtcgg gcagcctgcg gcaggctgat
     6361 gtaaatttca cccgcggtct cgtgctcaaa aaccggtata gctccctcga tgtaagaaaa
     6421 tgagaggaca tcggttgtac cctgcttgcc ccggtacgaa gcgttgagct tcgcgatctg
     6481 cgcgtctgtg accaactgga tatcgaatgt tacgccgcgc ttgagacccc gtggcttctg
     6541 ccgccagcgt gccgacgcaa ctgcggtttg aatggcgggc agaaggcgcg caaaggtccg
     6601 cacctgtgtg gcaaagcgct gtgggggctt ttttgaaagc aggcgaagag agatcaattg
     6661 cggaactggt cccgggcggt attgaggtcg gcaacgatgg caaagcggcc actcaggtcg
     6721 gtcatgtcga agagctcctg gacaaactgg gtcgggcgcg taagaaacat cttgccctgg
     6781 ttctgttcga cggtgcgcgc gatggtgagc agaagcgcaa tgcccgtgct attgatgtac
     6841 tcgagttgtg agatatcgag tatacagtca aagacgccat tttcaaagat ggccttaact
     6901 tttgaattaa gatcaaagat attgtcagag gtaattttgc cgctgaaaat cagcaggtta
     6961 acggagcggt taccaatttt ttccgtttcg gtccggagct gcagaagatt ctttaaaccc
     7021 actgtctcat tctgtctagc accctcatgg aatcaagctt tttaagctat ggcctgccgc
     7081 ggccggcgct gccgctgccc tttctaattg acgttctgat tcaagggccg gttttacccg
     7141 gtatggaatc atctggcaaa aaaactccgc tgattgacaa gctgctcatt ctgggcggtg
     7201 cagtgacatt tattggcctt actgtcacct tctcaccgat gctgtggatg gccacgctgc
     7261 tcgcgttgct gccggttctt gtcatggtag tactgattac cccgacgctg cacctgaata
     7321 tcgccgaaaa acagaccgaa gacaaccgcg cgaaataatt tccgccggca ggtgaagggc
     7381 ctgcaaacat gctaaaaaag ctcaagaccc tgcccctgct cagcaggagc aaatccggca
     7441 ttgttgacgt gccgaccgcg cttgccgcgt ttcgtgccgg caagatgctc attgttacag
     7501 attcagaaga ccgcgaaaac gagggcgacc tcgtgctctc ggcgaccgat actacgccag
     7561 aagccgttaa cttcatggcc aaatacgggc gtggcctcat ctgtgttccc gtcaccacct
     7621 cgcgcgcgaa agagctcaac ctgcaaccga tggtgcgccg taacgaagac gtacgccgca
     7681 ccgcgtttac cgtctctgtc gacgccaaag aaaagacatc taccggtatc tctgcacacg
     7741 accgtttttt cacaatacag aagctcgctg acccagcctc gaaggcgagg gatttcatgc
     7801 gtcctggaca catttttccg ctggtcgccc gcgacggcgg tgtactcgtg cgggccggcc
     7861 ataccgaagc cgcggtcgac ctctgccggc tgaccggaaa ggctccagtg ggagtcatct
     7921 gcgagatcat gaacagcgac ggcacgatgg cgcgcatgcc cgacctgatg aaattcgcgc
     7981 gcgaacacaa gctccccatt ctgaccatag aggcgctcat tgagtaccgc cgcaagtcag
     8041 agcagacagt aaccctgttg caaaagggca aactcaaaac acgctggggt gaatttaccg
     8101 cctgctatta caactcttcg acagacgaca acgtgcacgt ggcgcttgta ctcggcaagg
     8161 tcgacggcaa caccccggtc accgtgcgcg tacaccgcga agatgtcttt gccgatgttt
     8221 tccacagccc cacgcgcaag aactacctgc ccatcgacga cgccatggca gcgttgctca
     8281 agagcaaaaa gggagttttc attttcctgg gacaaaagac ctcgcagatt ttcggctctg
     8341 aatcttctga cggcgattcg gggctcaggc aatacggtat cggtgctcag atactcaacc
     8401 agcttggcgt gaagaaaatc cggctgctga cacgcaaccc gcgcaaagtt gttggtctcg
     8461 aagctttcgg cctcgagatc acggaaaccg tctcgcgcta aagcttgcgc gcggcgggcg
     8521 aaagccacac gttctatgat tatacatt
//
LOCUS       DAOQJF010000121         8482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4500_length_8482_cov_5.290495, whole genome shotgun sequence.
ACCESSION   DAOQJF010000121 DAOQJF010000000
VERSION     DAOQJF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_8482_cov_5.290495"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLF85_07865"
     CDS             <1..454
                     /locus_tag="PLF85_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19620.1"
                     /translation="HREAHYNPRLVMVTAHCGISATVLEEQLALFWLAETGLRTWQRL
                     LLAEALPSMEQIDRFRLVIAKPTKRRTPLVQVLLSDHLPETDERQALGGFVAKLAATG
                     KLKDFLTGLYRDRNADGTCLDTLEALFPGISDDILSNREPDFSRYKSP"
     gene            498..2582
                     /locus_tag="PLF85_07870"
     CDS             498..2582
                     /locus_tag="PLF85_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNE19621.1"
                     /translation="MSAWLVAVSLAISGVTCLLCLHQIYKRLSGIKKSEAQELINRDE
                     PLFRRYLLRVTVFGLMVFLVALGFAYYLEFHRHGNTAPGVLVLYASAFAGFLLLANLT
                     ERLLGKMQNRTNELQRENISRLFRFFLYAGALLGLTSVLFTAGYSWYAQRIGWATNEM
                     WLLPAFSAAVLLFWLRFHGALLARTADYAYELMRRQEAILPLTGIHNPLWRLRQLFAV
                     VNRFFFYHLEYLVLCLVVLTVGHRIELQTSLASHGVPGLLLSVFALGAVASIAAFLIM
                     RVRDKTSPETFLWNIRIGYIAALSVQSIGCYLVFVVGFGWHIKYFWIILLGSLTAFLL
                     NLYSAIFVAENHKTARSLISAAASSVSTVVHRGIAAGMRGAAIPALIIAAVMSLVYLL
                     GIVDERGDARFVHGLFALALALTAMVSLFPVAQATALVMPLSSSAIARLRLRGRKEEN
                     MQLLTKFRNLRSVAMPSYVLHAKIFYAALVILIFLVYTQLIGDGGNATLFKHLTETAA
                     MLIGGIASYFLAARINELVLNLGPALVRETSRQFREISGLSSAEAEPDMQALWKTANH
                     YLSRKIGPLFLGTLALPALACFIGGNYGLTGYLLGFGFFGFLNSNSWLTTGAAWSSAR
                     HAAEADAEVSRHNAQLDALVQADIVGDSMHEAVAPTLASAMLMTLVAALLFAPATLEM
                     HEYLREWVKSAF"
     gene            complement(2592..3245)
                     /locus_tag="PLF85_07875"
     CDS             complement(2592..3245)
                     /locus_tag="PLF85_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNE19622.1"
                     /translation="MDYVLAGATGLVGTEVLRQLAKDAQNRVFCLGRRAPEVSGPSIH
                     FVEWDFERPIQWPGEKPKNPHAICTLGTTIKKAGSQEEFRRVDLEFVIQFGREMQKQG
                     AQSLHVVTAHGASASSVIFYNRVKGQVEQQLSQLGLPALHIYRPSLLLGHRREHRAGE
                     GAATVIAKLLRPVFKLPGLNNVEPTPADRLAQFILRQCSAGKTGELIHSNYEILHGS"
     gene            complement(3306..4160)
                     /locus_tag="PLF85_07880"
     CDS             complement(3306..4160)
                     /locus_tag="PLF85_07880"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNE19623.1"
                     /translation="MQQSSATLKNPVDGFEFYTQNWKEGKTKPKKVIVVQHGFGEHSG
                     RYQNLIAALEGEKASVYALDARGHGKTPGKRGHISDFNLYASDLAVLVAKARAENKGV
                     PIILLGHSMGALIATLAALKSEVATELSGLILSSGAFKPALDTVQAIKKAVGTVLAKV
                     APAVTVPAGLDVNLISRDANVVQAYSNDPLVHGKISMKMGVDLFATGEALLEQASRLT
                     LPVLVFHGDADGIAQALGSKEFFQALSSKDKTLKIYPGFYHETMNEPFADRKQVLNDV
                     VKWINKHV"
     gene            complement(4210..4605)
                     /locus_tag="PLF85_07885"
     CDS             complement(4210..4605)
                     /locus_tag="PLF85_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19624.1"
                     /translation="MSTGNQSWQLRVIRVLFKVLALLTIDLVVQIVNIQIMDLQKYIA
                     PVYVTLLGMLLVVLLFYFLMAYIEQWTNALLKYVVELGKTFRYRKTAVMAIILTLMFC
                     AFFAYYRLWFGKWIGFSDLPKVFSAPILR"
     gene            complement(4602..6155)
                     /locus_tag="PLF85_07890"
     CDS             complement(4602..6155)
                     /locus_tag="PLF85_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19625.1"
                     /translation="MPQTAFHNFFSYIHRQRWALFLYLSALIHLSLFTHLAIRGTTVQ
                     EMEICNKPMIQLMAQVVPPPPGQQGEKSEDGGGFEHGPMADSGKNGQGDFAEQFGIPG
                     GKWKEQLDRTEEGEELNDSYGGKEDDKALVNPAVPEEYRKRKRDYRNIIAKDIFPTLN
                     SIDKPFRDEIKQAPQELEKMQERNEVIENYRLWREGKAIPDRKRARVVINAPRAGTQG
                     ALAFDEKARRRYFDETLSDPKEQQLERFLKDYGNYDPNDGDLPRAIRDLYYENLQRVA
                     SSFSPDMTYLAIDYYHEALNKEEFLRKALDVASKKKGTRYATEILFAIENIYEIQGRA
                     LKLLFEVKKHIEGLSAKEKENIRVKTLEKVVARYIPLAAKKGIRSERDALQHYFLKRT
                     EILDYLLENTPGGYRSKDATFAKGKVFWESAHTDPTNPAAMQQVKRAVDIWRTIDYLA
                     DAGDFVAGEAHSSMKDALAKFRGPGSPEYTQIRITLDRELHKLMQKKTERENRILWSS
                     KQTNKAAAK"
     gene            complement(6247..>8482)
                     /locus_tag="PLF85_07895"
     CDS             complement(6247..>8482)
                     /locus_tag="PLF85_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNE19626.1"
                     /translation="LFTYSKRVVKQRLVNHDLWIAEGIAENAPHATIGTASVQQLRLQ
                     QLASPSTIDHYQDAPQLTDFLTWASKIVGYLQSNLFFNYLRHRAEMNATGSSATMLTQ
                     LMTIQDQTINGLESVIPTYIPGANFPTLYADYVVTNYLMQLGIPINATTGWNNGAADQ
                     TKYSMANVNIGHSGSTVNGTTVKAKYPNVIPFNYEAPKCADGTYGLRPNSYIIFRHKF
                     DGNDKDGWDEAGTGPVAGEQRLRYVINIDSENSIISGSPPTTVRLREYTANDLATPTN
                     LPLGSGGYGLNLNDFVHMIVYNPNKSGSCRRFNTALISRRNHSKWVGFNTLGTQPSPD
                     FEWQSETGARWVNNQNGSYYRPGGIAAFAGGAYPNNFIYITDYTNMSVQKVNLDDGTA
                     VGRLGSTAVGCPSSGSGWTTNIERYANNYCSHSFDSPQGIHADTSHNIYVADTQNRRL
                     VKYNSAGVFQGWLGRNGAGNDTWQASGTEMAPIDLYNNGSVDDPRMFLLPWTMTSDAT
                     YLYVVDYGARRIVRRLLSTGAFSAYIGNGNNAWNTSTASQSGTSGSTAGYFTDPRGIV
                     IVGTNMYIVDEGNHRIVRVNVSTGASNAWIGDGDGWQTMGTAAPGSGSNLPKRFNRPS
                     AIASDGTYLYIADRLNDRISRWNVSTGNFAGWVGHGKVGWEMTASAPATDPYAGVSYY
                     PPDYYAEPHGLAMVTSTQKGTKNNYLYVTSVYNGRVTRINISCVDAPSSDACNPNLAN
                     PFTP"
BASE COUNT         1720 a         2358 c         2335 g         2069 t
ORIGIN      
        1 ccaccgcgaa gcccactata atccccggct ggtaatggtt acagcgcatt gcggcatttc
       61 ggcgaccgtg ctcgaagaac agctcgcctt gttctggttg gccgaaacag gtttgcgcac
      121 ctggcagcgc ctgctgctcg ccgaagcgtt gccaagcatg gagcagatag accgctttcg
      181 cctcgtcatc gctaagccca caaaaaggcg cacaccactg gtgcaggttc ttttatctga
      241 ccatttaccc gaaacggacg agaggcaggc gctgggcggc tttgtggcga agctggccgc
      301 gaccggcaaa ttgaaagatt ttctcacagg gctctaccgc gaccgcaatg cagacggtac
      361 gtgcctggat acgctggagg cgctgtttcc gggtatttca gacgatattt tatcgaaccg
      421 cgagcctgat ttttcccgat ataaatcccc ttaataacac tttacgcaag ctcgaaaaat
      481 tgcaggctac gcacctgatg tctgcctggc ttgtcgccgt ttcgctggct atttcgggtg
      541 taacctgcct gctctgcctt caccagatct ataagcgcct gagtggcatc aaaaaatccg
      601 aagcgcagga gctcattaac cgggacgaac cactctttcg ccgctacctg ctccgcgtga
      661 cggtgtttgg cctgatggtg tttctcgtcg cactcggctt tgcctattac cttgaattcc
      721 atcggcacgg caatactgca ccgggcgtgc ttgtgctcta tgccagcgct ttcgccggct
      781 ttcttctact cgccaacctc acggagcgcc tgctcggaaa gatgcagaac cgcaccaatg
      841 aactgcaacg tgagaatatc agccgtctct ttcgcttctt tctgtatgcc ggcgcattgt
      901 tgggattaac ctcggtgctg tttactgccg gttatagctg gtacgcccag cgtatcggtt
      961 gggcgacgaa cgaaatgtgg ctgttgccgg cattttctgc tgcagtatta cttttctggc
     1021 tgcgctttca cggagctctg ctcgcgcgta ccgccgacta tgcctacgag ctgatgcgcc
     1081 ggcaagaagc catactgccg ctcacgggta ttcacaatcc gctttggcgg ctgcgccagc
     1141 tttttgccgt ggtgaaccgg tttttctttt accatcttga atacctggtg ctctgcctcg
     1201 tcgtactgac ggttggccat cgcattgaac tgcagacaag cctcgcatcg cacggcgtac
     1261 ccggtttgct gctaagcgtt tttgccctgg gcgcggttgc ctcgattgcg gcgtttctga
     1321 tcatgcgcgt gcgggacaaa acctcgcctg agacattcct gtggaatatc cgtatcggct
     1381 atattgctgc cctgagtgtg cagtcgatcg gttgttacct ggtattcgtg gtcggatttg
     1441 gctggcacat caagtatttc tggattatcc tgctgggatc gctcactgcc ttcctgttga
     1501 atctctattc cgcgatcttc gtcgccgaga accataagac cgcgcgcagc ctcatttctg
     1561 cagcggcaag ctctgtatcc actgtcgtgc accgcggtat cgccgcgggc atgcgcggtg
     1621 cggcaatacc agcgctgatt atcgcggcag tgatgtcgct ggtttacctg ctgggtatcg
     1681 tcgacgagcg cggcgatgcg cgcttcgtgc acggcctgtt cgcccttgcg ttggcattga
     1741 ctgcgatggt ctcgctgttt cccgtagcgc aggcgacagc tctagtcatg ccgctttctt
     1801 cttcggcaat tgcacgcctg cgcctgcgtg gcagaaaaga agagaatatg cagctgttga
     1861 ccaagttccg gaacctacgg agtgtcgcga tgccttctta tgttctgcat gcaaagatat
     1921 tctacgctgc cctggttatc ctgatctttt tggtctatac acagctgatc ggcgatggcg
     1981 gtaacgcgac gctgttcaag cacctcaccg aaacggcggc catgctcatt ggcggcatcg
     2041 cttcttattt tctggccgcg cgcatcaatg aacttgtgct caatcttggc ccggcattgg
     2101 tacgcgaaac cagccgccag tttcgcgaaa tttcgggcct gtcatcggca gaagctgaac
     2161 ccgatatgca ggcgctctgg aaaaccgcaa accattacct ttcacgcaag atcggaccgc
     2221 tgttcctcgg tacactcgcc ttgccggcgc tcgcctgctt catcggcggc aactacggcc
     2281 tgaccggtta tcttttaggt tttggttttt tcggctttct caatagtaac agctggctga
     2341 ccaccggggc agcctggtcg agcgcccgcc acgccgccga ggcagatgca gaggtttcac
     2401 gccacaacgc acagcttgat gcgctggtgc aggcagacat tgtcggtgat tcgatgcacg
     2461 aggctgtagc acccacgctg gcctcggcga tgctgatgac tctcgtggcc gcgctgctct
     2521 ttgcgccggc aacgctcgag atgcacgaat acctgcgcga gtgggtaaag tcagctttct
     2581 aacaggccct ttcaggagcc gtgcagaatt tcgtagttcg agtgtatcag ctcgccggtt
     2641 tttccagcgc tgcactggcg caagataaac tgtgccaggc ggtcagccgg ggtcggttcg
     2701 acattgttta agcctggcag cttgaagacc gggcgcagca gttttgcgat gacggtcgcc
     2761 gcaccctcgc cggccctgtg ttcgcggcgg tgccccagta agagagaagg acggtagatg
     2821 tgcagcgcgg gaagaccgag ttgtgacaac tgttgctcaa cctgaccctt cacccggttg
     2881 tagaaaataa ctgaactggc ggatgcgccg tgcgccgtca cgacgtgcag cgactgtgcg
     2941 ccctgcttct gcatctcgcg cccaaactgg attacgaatt cgagatcgac acgccggaat
     3001 tcttcctggc tgccggcttt ctttattgtt gtacccagtg tgcagatcgc gtgcggattt
     3061 ttcggctttt caccgggcca ttggataggt cgctcgaaat cccactccac aaaatggatt
     3121 gaaggacccg aaacttctgg tgcgcgccgg ccaagacaga aaacccggtt ctgcgcgtct
     3181 ttcgccagct ggcgcaggac ttcggtgccg acgagtcccg tggcgcctgc gagtacgtaa
     3241 tccatgacac caagtagccg gggcggtcag gctgccaatc tatttggctg ttatggctgg
     3301 ctgattcaga cgtgcttgtt gatccacttc acgacatcgt tgagtacctg tttgcggtcg
     3361 gcaaacggct cattcattgt ctcgtggtag aaaccagggt aaatcttcag agtcttgtct
     3421 tttgagctca gcgcctgaaa gaactctttg ctgcccagcg cctgtgcgat gccgtctgca
     3481 tcgccgtgga agacaagcac tggcagcgtc agacggcttg cctgctcaag cagcgcttcg
     3541 ccggttgcga acaggtcgac ccccatcttc atcgaaatct taccatgcac aagaggatca
     3601 ttggaatacg cctgcacgac attcgcatcg cgcgaaatca ggttcacatc gagacctgcc
     3661 ggtacagtaa ccgcgggagc aactttcgcc agaacggtac caacggcctt cttgattgcc
     3721 tgcaccgtat cgagcgcggg cttgaaagcg ccactggaga ggatcagccc actgagttcg
     3781 gtcgccactt cgctcttgag cgcagccagc gtggcaatga gagctcccat cgagtggccg
     3841 agcaggataa tcggcacacc tttgttttca gcgcgcgcct tcgctaccag gaccgcgaga
     3901 tcagaagcgt agagattgaa gtcgctgatg tgaccacgtt tacctggtgt tttgccgtga
     3961 ccgcgtgcat cgagtgcgta gacgctcgct ttttcgcctt caagagcggc gatcagattc
     4021 tggtaacgac ccgaatgttc gccaaagccg tgttgcacga cgataacctt tttcggtttc
     4081 gtcttgcctt ctttccagtt ctgtgtatag aattcgaaac cgtcgacagg gttcttgagg
     4141 gtagcggagc tctgctgcat gggcaaacgt cgcagtgcgg ttcacggggt aaacccaaat
     4201 ggcatttggt cagcggagaa tgggagcaga aaaaactttt ggcaggtcgc tgaagccaat
     4261 ccacttaccg aaccagagcc gatagtaggc gaaaaatgca cagaacatca gggtaaggat
     4321 aattgccatg accgctgttt tgcggtagcg gaatgtttta cctagttcga ccacgtattt
     4381 cagcagcgca ttggtccatt gctcgatgta cgccatcagg aagtagaaca gcagcacgac
     4441 cagcagcata cccagcagcg tcacatacac cggggcgatg tacttctgca aatccatgat
     4501 ctggatattc acaatctgca ccacgaggtc gatcgtgagc agggcaagaa ccttgaagag
     4561 tacgcggatc actcttaact gccagctctg gttgcctgta ctcattttgc tgccgccttg
     4621 ttagtctgct ttgaagacca cagaatccgg ttttcacgct cggtcttttt ttgcatcagc
     4681 ttatgcaatt cgcggtcgag cgtgatgcgt atctgcgtgt actcgggcga acccggcccg
     4741 cggaatttgg ccagcgcatc tttcatgctg ctatgcgctt caccggcaac gaagtcaccg
     4801 gcatcggcga ggtaatcgat cgtgcgccag atatcgactg cgcgcttcac ctgctgcatg
     4861 gctgccggat tcgttgggtc ggtgtgcgcg ctctcccaaa aaaccttgcc cttcgcgaaa
     4921 gtcgcatctt tgctgcggta accacccggc gtgttctcaa gcaggtaatc gagaatttcc
     4981 gtacgcttga gaaagtagtg ctgcagagca tcgcgttcgc tgcggatacc tttctttgca
     5041 gccagcggta tgtaacgcgc gacgactttc tccagcgtct tcacacggat attttctttc
     5101 tctttggctg ataatccctc gatgtgcttc ttcacctcga acagcagctt cagggcgcgt
     5161 ccctgtatct catagatgtt ctcaattgcg aacaagatct cggtggcgta gcgggtgccc
     5221 ttcttcttcg aggcgacatc gagcgccttg cgcagaaact cttctttgtt gagagcttcg
     5281 tggtagtagt cgatggccag gtaagtcatg tccgggctga aagacgacgc gacgcgttgc
     5341 aggttttcgt agtagaggtc gcgtatcgcg cgcggcaggt cgccgtcgtt ggggtcgtag
     5401 ttgccatagt ccttcaggaa acgctcgagc tgctgctcct tcggatcgga aagtgtttca
     5461 tcaaaatagc gacggcgcgc cttttcatcg aaggcgagcg cgccctgtgt gccggcccgc
     5521 ggtgcattga tgacaacacg cgcccgcttc ctatcgggta ttgcttttcc ctcacgccac
     5581 aggcggtagt tctcgataac ttcgttacgc tcctgcatct tctcaagctc ctgcggggcc
     5641 tgtttgattt catctcggaa cggcttatca atcgagttca gggtcgggaa aatatctttc
     5701 gcgatgatgt tacggtagtc acgcttgcgc ttgcggtatt cttcgggaac cgccgggttc
     5761 accagcgcct tgtcgtcttc tttgccgcca taactgtcat tgagctcttc accctcttcg
     5821 gtgcgatcaa gctgctcttt ccacttgcca cccggtatgc cgaactgctc ggcaaaatca
     5881 ccctggccgt tcttgcctga atcagccatc gggccatgtt caaaaccgcc accatcctca
     5941 gacttttcac cctgctggcc tgggggcgga ggtacaacct gcgccatgag ctgtatcatg
     6001 ggtttgttgc agatctccat ttcctgcact gttgtaccgc gaatggcgag gtgggtaaag
     6061 agcgacaggt gaatcagcgc agagaggtaa aggaacagcg cccagcgctg gcggtggatg
     6121 taggaaaaaa agttgtggaa ggcagtctgc ggcatttcag ttatcaaaaa agcacccgga
     6181 tgggtgctgc cactgtgacc gaaatgacca gcgctctgtc aatgagattg gcatgcattc
     6241 ataccgttac ggggtaaacg ggtttgccag gttgggatta cacgcatcag aagagggagc
     6301 gtcgacacaa ctgatattga tgcgcgtcac ccggccgttg tagacggaag tcacgtagag
     6361 gtagttgttc tttgtccctt tctgcgtgct ggtaaccatc gcaagtccat ggggttctgc
     6421 atagtagtca ggcggataat aactcacacc ggcatagggg tcggtagcag gcgcagaggc
     6481 tgtcatttcc caacctactt tgccgtggcc aacccaaccg gcaaaatttc ccgtcgagac
     6541 attccaacgg ctgatgcggt cattgaggcg gtcggcaata tagagataag tgccgtcgct
     6601 cgcaatggcc gatggccggt tgaagcgttt cggcaggttg ctacctgaac ctggtgcggc
     6661 ggtacccatc gtttgccagc cgtcgccgtc accgatccag gcattggaag caccggtaga
     6721 tacattgacc cggacaatcc ggtggttacc ttcgtcgacg atatacatgt tggtgccaac
     6781 gatcacaata cctcttgggt cggtaaaata accggcagta ctgcccgagg tacccgattg
     6841 cgacgcagtg gaggtattcc aggcgttgtt gccattgccg atgtaggcgc tgaaagcacc
     6901 ggtagaaagc agccggcgca cgatgcgccg ggcaccgtag tcgaccacat agagatatgt
     6961 agcatcagaa gtcatcgtcc acggcagcag aaacatgcgc gggtcatcaa cggatccgtt
     7021 attgtacaga tcaatgggtg ccatctcagt acctgaagcc tgccaggtat cattgcctgc
     7081 tccgttacgg cccaaccagc cctgaaagac acctgcagaa ttatacttca ccaaacgccg
     7141 gttctgcgta tcagcaacat atatattgtg agaagtatcc gcgtgaatcc cctgcggcga
     7201 gtcaaaactg tgcgagcagt agttattggc ataacgttct atgttggttg tccagcccga
     7261 gccagaactc ggacaaccga ccgccgtgct gcccaatctg cccacagcag taccatcatc
     7321 gaggttcact ttttgcacgc tcatgttcgt gtaatcggtg atgtagatga agttgttggg
     7381 gtatgcgcca cccgcaaatg cggcaatacc gccgggacga tagtagctcc cgttctggtt
     7441 attcacccac cgtgccccgg tttcactctg ccattcgaag tcaggcgaag gctgtgtacc
     7501 aagcgtgtta aaaccgaccc acttgctatg gttacgccgc gagatgagcg ccgtgttaaa
     7561 ccgccggcag ctacccgact tgttggggtt gtacacgatc atgtgcacaa agtcgttgag
     7621 gttcagacca tagccgccac tgcccaaggg cagattggtg ggggtcgcca ggtcgttggc
     7681 agtatactcg cgaaggcgca cggttgtggg agggctgcct gaaatgatgg agttctcgct
     7741 gtcgatattg atgacatagc gcagacgctg ttcacccgcg acagggcccg tgccagcttc
     7801 gtcccaacca tctttgtcgt taccgtcaaa tttatgccgg aagatgatat aagaatttgg
     7861 tctcaggcca tacgtgccgt cagcgcattt cggagcttcg tagttgaagg gtataacatt
     7921 cggatacttg gccttgaccg tcgtgccgtt gactgtcgaa cccgagtgcc cgatattcac
     7981 gtttgccatg ctgtactttg tttggtctgc tgccccattg ttccagccgg ttgttgcatt
     8041 gatcggtatg ccgagttgca taaggtaatt cgtgacaacg tagtcggcat agagggtcgg
     8101 gaaatttgcc ccgggaatgt aagtcggtat cacactctcc aagccattaa tcgtctggtc
     8161 ttgtatcgtc ataagctgcg tcagcatcgt cgctgaacta cctgtagcat tcatctctgc
     8221 acggtggcgc aggtagttga agaaaagatt actctgcagg taacccacaa tcttcgaagc
     8281 ccaggtcaaa aagtctgtca gctgcggagc atcctggtaa tgatcgatcg tcgacggcga
     8341 tgccagctgc tgcagccgca actgctgcac gcttgccgtt ccgatggttg cgtgcggtgc
     8401 attttctgca ataccttcgg caatccacag gtcgtggttc acaagccgct gtttgacgac
     8461 ccgcttgctg taagtgaaaa gg
//
LOCUS       DAOQJF010000122         8463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4524_length_8463_cov_5.710395, whole genome shotgun sequence.
ACCESSION   DAOQJF010000122 DAOQJF010000000
VERSION     DAOQJF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4524_length_8463_cov_5.710395"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..963)
                     /locus_tag="PLF85_07900"
     CDS             complement(112..963)
                     /locus_tag="PLF85_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE19627.1"
                     /translation="MPGALQLLKALSEPVRIRLYLLLRQSSLTVSELSEILALSQSNT
                     SHHVKALRELDLLTAEKTGQHTYYALNRSTLADSRIAAVLKTLEDAAAEISELPADNA
                     RLRNTLAARSEDTFARWRMEQPDLPYSDIFAHLACGRRGRVIDIGCGEGDFFEPLGLS
                     FGQVVAVDLDFGHTRRARSRSAGQVTVYCADAQQLPFAAAAFDAVVLRMALSQMPDHR
                     AALAEALRVLKPGGFISVIDSDTDSNRTLRNSVVSFFTETPLLQLDTERALPRLFMLR
                     AQRRLLR"
     gene            complement(965..1654)
                     /locus_tag="PLF85_07905"
     CDS             complement(965..1654)
                     /locus_tag="PLF85_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19628.1"
                     /translation="MSAFFPRALRAARAAAICLAFWVSSPAWSVGASFDMPGKTPEEA
                     VDTAKRALKRLNTDCVEQDITLGAHWRCKTSFFSLVGLDVFVSTIPDKAIIRADSRNR
                     QSFAFVDLVAHESGQGPFANTYGEKSLLLTAGATLISPALGYWYVNSNSMIKNKSIFL
                     PMLGLFFGDLALFWVSSKIYFTNGFDPFDVGLTSMLISMGTFRAVMLVPLSVQVLAHN
                     RFVGLQITFRY"
     gene            1763..2008
                     /locus_tag="PLF85_07910"
     CDS             1763..2008
                     /locus_tag="PLF85_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNE19629.1"
                     /translation="MSPELLEILVCPRTKKKLQIADTAVLERVNALIKSGKCKEVSGA
                     QVSEPATAGLYEPENQTFYFIREDIPVLVYDNAVRLG"
     gene            2030..3961
                     /gene="dxs"
                     /locus_tag="PLF85_07915"
     CDS             2030..3961
                     /gene="dxs"
                     /locus_tag="PLF85_07915"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNE19630.1"
                     /translation="MNAISSNQPDFAQVDSPEKLKQLQQAQLPAFCEYLRGYILETVA
                     ANGGHLSSNLGSVELTVALHYVLKSPFDKILWDVGHQCYAHKILTGRKKQLPTIRKTG
                     GLSGFPKRSESKHDLYNTGHAGTAISQAMGEAIAARLTAKPGAPLPTVAAVVGDASIV
                     TGMSFEAMNHAGYARTPMLVILNDNEMSISKNVGAISYRLTQLINTRLYRKSKRGFIN
                     LVAKIPLIGRVINRLAKRFRMSMKSLATDHQFFGELGFRYLGPIDGHDVLKIVHLLES
                     LPPIEEPILLHVVTKKGYGYELAEKDPIQYHGVGVFDTGKGIEKKKDAPWGLSKFVGE
                     TLAHLGEKDRRVTVITPAMKEGSGLGPFAAKFPERFFDAGIAEQHATTFAGALASAGL
                     KPYLCIYSTFLQRGYDQLVQDIALMNLPVRLVIDRAGCVGQDGETHQGFYDIAFMAAL
                     PNIRILSARNAEELVKMLAFQKDYDAAPLAIRFPKKEYAAFDIAAFVKHAGLASYNPF
                     TAEVLAPGKQVLLLSEGTFADKALAVRELLQAQKISAEVMDLRCLRPLPVAQLLRALR
                     GKKLLVTLENHGLTGGVGDLVGQALRQKGVAIRHLALGYPEEVVPHGSISDIEDIYGL
                     TPQKIAAQIVRAMRSKPSR"
     gene            complement(3958..5391)
                     /locus_tag="PLF85_07920"
     CDS             complement(3958..5391)
                     /locus_tag="PLF85_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19631.1"
                     /translation="MAGEALKPFNFSPEIIARMRETQEIPVNFYNANGQVLIPKKEQA
                     SADLINKLLQHIGSGIFYRESDEDRLGIKKTGREDLEGLSDTKLLSEKRVAELSAATE
                     TLFSELKFAAFGAVHSQKMHSQVNSFISDFEQQPDMMVGVINILDTVKGGSSELTKLA
                     VKRAVVAMALKSRSMKAMRSKDRGRGSEAVQPVMMSGMLSQIGKTKMNLPEGENLTSE
                     QRAYLRKYPLLSYMMIAHEPTVTFETKRLVLNQRRTLPENQHANNYPELRWMVATLQT
                     LSQDNEKKGKKEVASDIVKQLTSLKEFVVYEEDVNILSLATDFAALTTETAWREAKDP
                     LTAIKLIVNTSFYQYGPKVIRDFLDHISISLSHNQKVIKNDDLLILSLTMNSGQTYFE
                     VVKVLEVGRYQSRALVQRLGVLHMNTTRENGLYRGQFMTETFRPDPRRIKINLAQDFL
                     RRIIYVVDPVIDPQLYGQLQQKIHPMG"
     gene            complement(5493..6494)
                     /gene="ilvC"
                     /locus_tag="PLF85_07925"
     CDS             complement(5493..6494)
                     /gene="ilvC"
                     /locus_tag="PLF85_07925"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNE19632.1"
                     /translation="MAKLYYDQDCDLSVLKNSTIAVIGYGSQGRGQAQNMMDSGLKVI
                     IGLKAGSQSTAEAKSDGFEVVDVATAAQRADVIQILTPDQTQKEIFESQIAPHLKAGK
                     ALVFSHGFNIHFDEIVPPKDVDVYMVAPKGPGHLVRRVYTEGGGVPCLIAVHQDATGK
                     AKARALAHASAVGGGRAGILETSFKEETETDLFGEQAVLCGGISHLIQAGFETLIEAG
                     YDPEIAYFECLHETKLIIDLIYEGGLEKMRYYVSDTAEYGDYVSGPRVIDGHVKQKMK
                     EVLADIQKANGSKFAKGFLADLRTGGKEFESMRKKEENHPIEKVGKKLRSMMRWLSK"
     gene            complement(6541..>8463)
                     /locus_tag="PLF85_07930"
     CDS             complement(6541..>8463)
                     /locus_tag="PLF85_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNE19633.1"
                     /translation="ATGGATICYTTNGVDPGCTLGLCSGSTTYGAPVAISADTNLKAI
                     GCAISYSNSAIQSGAYVIDMTGPTTPGTPSASPVSTTQISVTWSMSSDNTTPTGSIVY
                     EVCLATAPAVCDAFAVNATTGPGATGYSASALSPGTTYYFRVRARDQFNNLGAPTAEF
                     SATTASLPQVLSVTPADNTANVATNALIVITFNKTINTGTATAQTTSGPCTNTLQISA
                     DNFVTCIGISSVNWDGTQENATLTPAQPLQFLTQHRILVLSSVTDTFGNPMAANFTTP
                     IGFMTKAPDPATLDLSFNPGTGANGSVNAIDIHTSGNILIGGVFLDYNGFGSTNIAYI
                     QPNGNLDPGWFTTGADGEVHSVRARNDGSIFLGGAITSLNGFGRDRIARAFLSGDNDG
                     SFNPGVGFDNTVSGLVINGNEITAVGSFTTFNATGRSRIARIFDDGTIDGSFTPGTGA
                     NGDVYAILLQPDTTLIIGGLFTSYNGTARNRIARLFSNGTLDTGFDPGTGADNKILAL
                     ARQGDGKLIVAGTFTTFNGSARNSIARVNGDGSHDLTFNPGTGANGVINAVALQTDGK
                     IIIGGNFTTFNGFTRNAIARLNNDGSLDTLFDPLSGPDGHVSAIAIQADNKILIGGGF
                     TTYAGVPRQGIARIHP"
BASE COUNT         1889 a         2255 c         2355 g         1964 t
ORIGIN      
        1 cgcagcttac ccgaaataaa gcagtttctc gagggcgatc aaaagcaccg ttcatacgtt
       61 ttgatgacgc aaaatgaccg cgacgacctt gtggtttacc tgcgcagctt gttaccgcaa
      121 taacctgcgc tgtgcgcgca gcatgaatag tcttggcagc gcacgttcgg tatcgagctg
      181 caacagcggt gtttccgtaa aaaaagaaac gactgaattg cgcagggtgc ggttgctgtc
      241 ggtatcgctg tcgatgacgc tgataaagcc gccgggcttg agaacgcgca aagcttcggc
      301 gagtgctgcc ctgtgatcgg gcatctgcga cagcgccatg cgcagcacta cggcgtcaaa
      361 ggccgctgcc gcaaaaggca gttgctgcgc atcggcgcaa tatacagtaa cctgtcctgc
      421 agaccggctg cgcgcccgcc gggtatgccc gaagtcaagg tctacagcga ccacctgccc
      481 aaaagacagg cccagtggtt cgaaaaaatc gccttcgccg caaccgatat cgataacccg
      541 tccccggcga ccgcacgcaa ggtgcgcaaa gatatcgcta tagggcaggt cgggctgctc
      601 catgcgccag cgtgcaaaag tgtcttcgct gcgcgccgca agcgtgttgc gcaggcgcgc
      661 gttatctgcc ggtaactcgc tgatttctgc tgccgcgtct tcgagcgttt tgagaaccgc
      721 ggcaatgcgt gaatcggcca gcgtcgagcg gttcagggcg tagtaggtgt gctggcctgt
      781 tttttctgca gtcagcagat cgagttcacg cagcgctttc acatggtggc tggtgttcga
      841 ctgcgagagg gcaagtattt cagacagttc tgaaaccgtc agcgagctct gccgcagcag
      901 caggtaaagg cgtatacgga cgggttcgct gagtgccttg agcagctgca gggcgccggg
      961 catttcaata acggaacgtg atctgcagtc cgacgaatcg gttgtgtgcg agtacctgta
     1021 ccgaaagcgg cacgagcatc actgcccgga aagtacccat cgaaatgagc atagaggtga
     1081 gtcccacgtc aaagggatca aaaccgttgg tgaaatagat ctttgaactg acccagaaaa
     1141 gtgcgaggtc gccgaagaat agaccgagca tcggcagaaa gatcgacttg ttcttgatca
     1201 ttgagttcga gttaacgtac cagtagccca gagcaggtga aatcagcgtt gcgccagccg
     1261 tcaaaagcag cgacttttca ccgtaggtgt tggcaaaggg accttggccg ctttcgtgtg
     1321 ccaccagatc gacgaaggca aaactctgcc ggttgcgcga atcagcccgg atgattgcct
     1381 tatcgggaat cgtcgaaaca aaaacatcaa gaccaaccag cgagaagaag ctggttttgc
     1441 agcgccaatg tgcaccgagg gtaatatcct gttcaacgca atcggtattg aggcgcttga
     1501 gcgcgcgttt tgccgtatcg actgcctctt ccggagtttt gccgggcata tcaaacgaag
     1561 ctcccacaga ccatgccggg ctcgataccc agaaggcaag gcaaatcgct gccgcacggg
     1621 ctgctctcag agcacgcggg aaaaaggctg acatggcaca aggactcaaa gacgaaacag
     1681 cccgtcacgc ggcttctttg ccggtcgcat ggcatttggg ctctgcattg acggagtgtt
     1741 tccgccgccg tttctgccta ctgtgagtcc cgagctgctt gaaatcctgg tttgcccgcg
     1801 caccaagaag aagctgcaaa ttgccgatac tgcggtactt gagcgcgtta acgcgctgat
     1861 caagtcgggc aagtgcaaag aagtctccgg tgcgcaggtc agcgagcctg cgaccgcagg
     1921 gctctacgaa cctgaaaacc agacgttcta tttcatacgc gaagatattc cggtgctcgt
     1981 atacgacaac gcagtcaggc tcgggtaggt tgtcctggtt caaccagtca tgaacgcaat
     2041 ttcgtccaac caacctgatt ttgcacaggt cgacagccct gaaaaactga agcagctgca
     2101 gcaggcacag ttgcctgctt tctgtgaata cctgcgtggc tatatactcg agactgtcgc
     2161 agcgaacggc ggccatttgt cttctaacct cgggtcagtc gagctcacgg tggcgctcca
     2221 ttacgttctg aaatccccct ttgacaaaat tctctgggat gtcgggcacc agtgttacgc
     2281 gcacaaaatt ctgaccggcc ggaagaagca gctaccgaca attcgcaaga ctggtggcct
     2341 ctctggtttt ccgaagcgct ctgaatcaaa gcacgatctc tataacacgg gtcacgccgg
     2401 taccgcaatt tcacaggcaa tgggcgaggc aattgccgca cggctcactg ccaagcctgg
     2461 cgcgccgtta ccaaccgtgg cggctgttgt cggcgatgcc tcgatcgtga ccggtatgtc
     2521 tttcgaggcg atgaaccatg cgggttatgc gcgcacgccg atgctggtca ttctgaacga
     2581 caacgagatg agcatctcga agaatgtcgg tgcaatcagt taccggctga cgcaactcat
     2641 caacacccgt ctctacagaa agagcaaacg cggttttatc aatctcgtgg caaaaattcc
     2701 gctgataggc agggtcatta accggctggc aaaacgcttc cgcatgtcga tgaaatcgct
     2761 ggcgaccgac caccagttct tcggcgagct gggttttcgc tatcttggtc ccatcgacgg
     2821 gcatgacgta ttaaaaattg ttcacctgct cgaatcgctg ccccctatcg aagaacccat
     2881 actgctgcac gtcgtgacca aaaaaggtta tggctacgag ctcgcagaaa aagatccaat
     2941 ccagtaccac ggcgtgggtg tattcgacac gggcaagggc atcgaaaaga aaaaagatgc
     3001 cccgtggggt ctgagcaaat ttgttggcga gacgctggcg cacctcggtg aaaaagaccg
     3061 cagggttacc gttatcactc ctgcaatgaa agaaggctcg ggtcttggcc cgtttgcggc
     3121 aaaatttccc gaacgtttct tcgatgcggg tattgccgaa cagcacgcga caacctttgc
     3181 gggagctctg gcgagcgcgg ggcttaaacc ctacctgtgc atctactcca cttttttgca
     3241 gcgtggctac gaccagctgg tgcaggatat tgcgctgatg aacctgcccg tgcgcctggt
     3301 cattgaccgc gcgggctgcg tcggccagga cggcgaaact caccagggtt tttatgatat
     3361 cgcgttcatg gcagctttgc cgaacattcg catactctcc gcgcgcaacg ccgaagaact
     3421 ggtaaaaatg ctcgcattcc agaaagatta cgatgctgct ccgcttgcga ttcgctttcc
     3481 caaaaaagag tacgcggcgt ttgatattgc tgcattcgtg aaacacgcag gccttgcatc
     3541 ctataatcca ttcacggcag aagtgcttgc acccggtaag caggtcctgt tgctgagcga
     3601 ggggaccttt gccgataagg cgctcgccgt gcgcgagctg ctgcaagctc agaaaatatc
     3661 agccgaggtc atggacctgc gctgcctgcg gccattgcct gtcgcgcagc tgctgcgcgc
     3721 attgcggggc aagaagcttc tggtaacgct cgagaaccat ggactcaccg gcggggttgg
     3781 cgaccttgtt ggacaggcac tgcgccagaa gggcgttgcc attcgccacc tggcactggg
     3841 ttatcccgaa gaagtggtgc cccacggttc aatttctgat attgaagata tctatggcct
     3901 tacaccgcag aagatagcgg cgcagatcgt gcgcgcaatg cgttcaaagc ccagccgtta
     3961 acccattggg tggatttttt gctgcagctg cccgtacagc tgcgggtcaa tcaccgggtc
     4021 tacaacgtag atgatacggc gcagaaaatc ctgtgcgagg ttaattttga tacggcgggg
     4081 gtcagggcga aaagtttcgg tcatgaactg cccgcggtag agaccattct cccgggtggt
     4141 attcatgtgc agtacaccga gtctttgtac aagcgcccgg ctctggtacc tgccaacctc
     4201 gagtactttc acgacttcaa aataggtctg cccgctgttc atcgtcagtg acagaatcag
     4261 caggtcatca ttcttaatga ccttctggtt atgactcaat gaaattgaga tatggtcgag
     4321 aaagtcccga ataaccttcg ggccatattg ataaaacgag gtattgacaa tcaatttaat
     4381 ggcggtaagc gggtctttag cttcgcgcca cgcggtttcg gtggtcagcg ccgcgaagtc
     4441 ggtcgccagg ctcagaatat tgacgtcttc ttcgtaaacg acaaattctt tcagtgaggt
     4501 aagctgtttg acgatatctg aggccacctc tttcttgcct ttcttctcgt tatcctgtga
     4561 gagtgtctgc agggtcgcga ccatccagcg tagttcaggg tagttgttag cgtgctggtt
     4621 ttccggcaag gtacgtcgct ggttgagcac cagtcgctta gtctcaaaag ttactgtcgg
     4681 ttcatgcgcg atcatcatat aggagagcag cgggtatttg cgcaggtacg cgcgctgttc
     4741 actggtaagg ttctcccctt cgggaaggtt cattttcgtc ttaccaatct gcgacagcat
     4801 gccgctcatc atgactggtt gtaccgcctc ggaccctctg ccgcggtctt tcgatctcat
     4861 cgccttcatg cttcgcgact taagcgccat cgcaactacc gcgcgcttca cggcaagttt
     4921 tgtgagttct gaagagccac ctttcacggt atcgagaatg ttgataaccc caaccatcat
     4981 gtcaggctgc tgctcgaagt cgctgataaa tgagttcacc tggctgtgca tcttctgcga
     5041 atgcacagcg ccgaaagcgg cgaattttag ttcactgaaa agagtctcgg tagcggcgct
     5101 gagttccgcc acgcgttttt ccgagagtag tttagtgtca ctcaggccct ctagatcttc
     5161 acgcccggtc tttttgattc ccagtctgtc ttcgtcgctt tcgcggtaaa agatgccgga
     5221 gccgatgtgc tgcaagagct tgttgatgag gtcggctgat gcctgttcct ttttaggaat
     5281 cagtacctgc ccgttggcat tataaaagtt aacaggaatt tcctgagtct cgcgcatccg
     5341 cgcaataatc tctggagaaa agttaaaagg cttaagggct tccccggcca tacgcgtcag
     5401 agcgggatta cagagcgagg cagacggtca aggtaagaag agcacaagag agaacacagc
     5461 gcagccggtt ttggctgcgc tgtgactgga atttatttag agagccaacg catcatcgaa
     5521 cgcagcttct tgccaacttt ctcgatggga tgattctcct cttttttgcg catgctttcg
     5581 aattctttgc cgcctgtgcg caggtcagca aggaagccct tggcaaattt agaaccattg
     5641 gctttctgga tatctgccag tacttctttc attttctgct tcacgtgacc atcgatcacg
     5701 cgcgggccgc tgacatagtc gccgtattca gccgtgtcag aaacatagta acgcattttt
     5761 tccagtccac cctcgtaaat gaggtcgatg atcagctttg tttcatgcag acactcgaag
     5821 taagcgattt cagggtcata acccgcttcg atcagcgttt caaaacctgc ctggataagg
     5881 tggctgatac caccgcagag cactgcctgc tcgccaaaaa ggtccgtctc agtttcttct
     5941 ttgaatgaag tttcgagaat acctgcgcgg ccgccaccga ctgcgctcgc atgggcgagt
     6001 gcacgggctt ttgccttacc cgtcgcatcc tggtggactg cgatcaggca gggaacaccg
     6061 ccgccttcgg tataaacgcg gcgcaccagg tggccaggac ccttgggagc gaccatgtat
     6121 acgtctacgt ctttcggggg tacgatctcg tcaaaatgga tattgaagcc gtgcgagaaa
     6181 acgagagcct tgcctgcctt caggtgggga gcgatctgtg attcaaagat ttctttctgg
     6241 gtctggtctg gggtcagaat ctggattaca tcggcgcgct gcgcggctgt ggccacatcg
     6301 accacttcaa aaccgtcaga ttttgcttca gcggtggatt gagagccggc tttgaggcca
     6361 ataatcacct tgaggcctga atccatcatg ttctgcgcct gaccgcggcc ttgcgagcca
     6421 tagccgatga ctgcgatggt tgaatttttg aggactgaca ggtcgcagtc ctgatcgtaa
     6481 tagagttttg ccataaggtc aaaagggggt cagagacacc ccgtcaagga aagtgacccc
     6541 ttaggggtga atacgggcaa tcccctggcg cggaacacct gcataagttg taaaccctcc
     6601 gccgatgagg atcttgttat ctgcctgaat ggcaatcgcg ctcacatgtc cgtcagggcc
     6661 gcttagcggg tcaaacaggg tatccagtga accgtcgtta ttgaggcgcg caatcgcgtt
     6721 gcgagtaaag ccgttgaagg tagtgaaatt gccgccaata ataatcttgc catccgtctg
     6781 tagggcgact gcgttgatga caccattcgc acctgtgccc gggttgaagg ttaaatcgtg
     6841 cgaaccatcg ccatttacgc gggcaatgct attgcgtgcg ctgccattaa aggttgtgaa
     6901 agtacctgcc acgataagtt ttccgtcgcc ttggcgtgcc agggcgagaa ttttgttatc
     6961 tgcgcctgtg cccggatcaa aacctgtatc cagcgtaccg ttgctgaaca accttgcgat
     7021 tctgtttcgg gcggtgccgt tgtagcttgt gaacaggcca ccgataatca aagtcgtatc
     7081 gggctggagc agaatcgcat aaacatcgcc attggcacct gtcccgggcg tgaatgaacc
     7141 gtcaattgta ccgtcatcga aaatacgggc aatgcggctg cggcccgtag cattaaacgt
     7201 ggtaaagctg ccgacagcag taatttcatt cccgttgatg accaatccac tcacggtatt
     7261 gtcaaagccc acgcccggat taaacgagcc gtcgttgtct ccgctcagaa atgcacgtgc
     7321 tattctgtct cggccgaatc cattcagtga agtaattgca ccaccgagaa aaatgctgcc
     7381 gtcatttctc gcgcgtacag aatgcacttc cccatctgcg cccgttgtaa accagccggg
     7441 gtcaaggtta ccgttgggtt gtatgtaggc gatgttcgtg ctgccgaaac cattgtagtc
     7501 gagaaaaact ccgccaatga ggatatttcc actcgtgtgg atgtcaatag cattgacgga
     7561 gccgttggcg cccgtaccgg gattaaaact cagatcgaga gttgccgggt ccggcgcttt
     7621 tgtcatgaaa cctattggcg tggtaaagtt cgcagccatc gggttgccaa aggtatccgt
     7681 gaccgagctt aacacgagta ttcggtgctg cgtcagaaac tgcagcggct gtgccggcgt
     7741 aagggtcgcg ttctcctgcg tgccatccca gttgacgctt gagataccga tacaggtgac
     7801 aaagttgtca gcgcttatct gcagagtatt cgtgcacggc cccgaagtgg tctgcgcagt
     7861 tgctgtgccg gtattgattg tcttgttgaa ggtgatgaca atcagtgcgt ttgtggccac
     7921 atttgccgta ttgtctgcag gcgtaaccga cagtacctgc ggcaacgatg cagtggtggc
     7981 agaaaattct gccgtcgggg cgcccaggtt gttgaactgg tctcttgcgc gaaccctgaa
     8041 atagtaagtc gtaccgggcg ataatgcact ggcactgtag ccggttgcgc ccgggccggt
     8101 agtggcgttt accgcaaatg cgtcgcagac agccggcgcg gtcgcaaggc aaacttcgta
     8161 cacaatggac cccgtgggcg tcgtgttgtc agacgacatc gaccacgtca cgctgatctg
     8221 tgtagtgctg acggggctgg cactcggcgt acctggtgtt gtaggccccg tcatgtcgat
     8281 cacataggct ccgctctgga tcgccgagtt cgagtaactg atggcgcaac caattgcttt
     8341 gaggttggta tcggctgaaa tagctaccgg tgcgccgtaa gttgtgctgc cgctacagag
     8401 acccaacgtg cagccagggt cgacaccgtt ggtggtgtag cagatcgtcg caccacccgt
     8461 tgc
//
LOCUS       DAOQJF010000123         8446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4537_length_8446_cov_5.250030, whole genome shotgun sequence.
ACCESSION   DAOQJF010000123 DAOQJF010000000
VERSION     DAOQJF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4537_length_8446_cov_5.250030"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..214
                     /locus_tag="PLF85_07935"
                     /pseudo
     CDS             1..214
                     /locus_tag="PLF85_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
     gene            complement(265..1128)
                     /locus_tag="PLF85_07940"
     CDS             complement(265..1128)
                     /locus_tag="PLF85_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19634.1"
                     /translation="MADHLTFLPEKTPSRNLLVQIIFELLYGIRYLLLYIPGLFIRPG
                     DITEGDKRAVVLVSGFFGSPLTWLRCRAALAAQGHPVYVVRLGFQFGNLRKYSEKLQH
                     FLEENSISDCYLVCHSMGGLVAAHMGYRGRDRTRKIFTLGTPFRGHPLTFLFPFTFAS
                     LQMNPLSPFMRETKDKYRTLSSVQAIFARFDEVLFLNYLNIPGRFDDVKLAQVGHCNL
                     FMGPAGVECIADLIEAEEAKDPKPVVRKDDKAAAAQAATKTKPAKKSPTKTKKAAKPK
                     ARPAAKKKKRR"
     gene            1291..1899
                     /locus_tag="PLF85_07945"
     CDS             1291..1899
                     /locus_tag="PLF85_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19635.1"
                     /translation="MSIWRSSDRIALLEERYNRFIDEYAEAALLAGQAGVGFVDLLST
                     HKKEKFVHRTDVRPDEFNQYADEQAQWQLESGYSQQVKELETGLCELRTRILCHLRGG
                     AVTVDGDLLARVRGAHSNHRAEDRQQAIEWLTRQCEILAYYTDPKVARKARIDAAECS
                     LRLNLARSEISRMEQLPAHELLYDRSLTAIDTGRFEIPQRSR"
     gene            1978..3738
                     /gene="ilvB"
                     /locus_tag="PLF85_07950"
     CDS             1978..3738
                     /gene="ilvB"
                     /locus_tag="PLF85_07950"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNE19636.1"
                     /translation="MADGAKSAKETKTAEKNGSQVMIDTLLKNGIDICWGYPGGAILP
                     FYDELYHSKIRHILVRHEQGAVHAAEGYAKATGKVGMCIATSGPGATNLITGITDAKM
                     DSVPLLALTGQVATSVIGSDAFQECDTYGMSIPITKYNALLRSADDVARVTQEAITVA
                     GARRPGPALIDFPKDIQVQKTRVTKPEMRLDARHYKKPEMRGDFDKLIAAISKAERPL
                     LYVGGGAITSNAYKQIRELAEKADIPVYMTLMGLGAFPAQHRLSLGMAGMHGTAYANK
                     AIQECDFMLALGARFDDRVALNVDDYGKRAVKAMVDIDEAEINKRVPVDIHIVGDLVE
                     VLTHLNKNVKKQSRKAWTDRIVKLKEENPLRFNVEPGSIKPQQAIHELWKKTRGEAII
                     STDVGQHQMWAAQYYTFNEPRQWLTSGGLGTMGFGFPAAIGAALGRPEKDVFVISGDG
                     SFQMCIQELATVAMYKIPVKILLFNNGFLGMVRQWQELFYDNRFSQSDMNYNPDFVKI
                     CSGYDIPAKRIDKQSELSAGIDFLVNSKTSALLEVTIPEKEKVYPMIASGARYEQMVD
                     FVSKKEQGTEMKVHLNRPEK"
     gene            3812..4177
                     /locus_tag="PLF85_07955"
     CDS             3812..4177
                     /locus_tag="PLF85_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19637.1"
                     /translation="MTPVAAEGLPLLRSIFGNATLGEARFRETINKLNRYGEYAFAEA
                     YRSEQTLAFYSGKGNTSCWLKHHDSDDYADKLTQEIAEHKYPVFRYGNWLGYCTAGDT
                     EKLASQVRYILIGAGEWRE"
     gene            complement(4194..5003)
                     /locus_tag="PLF85_07960"
     CDS             complement(4194..5003)
                     /locus_tag="PLF85_07960"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19638.1"
                     /translation="MRQIAALLPLFFIACNSVFYQGDQKRWYPPEDFNINCSRRPIAT
                     ADGTSLMTMFCPAKGKTNRGIIIQFHGNAQNMNAHYRFLSWLIEEGYSLATFDYRGYG
                     ESTGESSRDGLRLDAQAFVREVRRQYSNERRIFYGQSLGGAVLMRTLIDEGLREDETY
                     IFEGTFLSYQAVARTALASRWFLWPLQPLAWVLVTDTHAPDDDASAFAGKRVILIHGE
                     ADPVVPISHGEKISRTMQQPLWRIKGGRHLDTWHIEHGRLRAELLQKLAEK"
     gene            complement(5005..6900)
                     /locus_tag="PLF85_07965"
     CDS             complement(5005..6900)
                     /locus_tag="PLF85_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNE19639.1"
                     /translation="MAFFATAGLSAEHPIQQTLRELAAQQYYTNARQLKLADTAGWRR
                     LIHYDSFLGSLRSEARDKTPGEFFASAKGERDPEAELEATLLALFTDNYSAEPGRCRY
                     PAREIFLVRELKIDTKKLPVVDCKKYREWKEHISADSASIVFASFFMGNPSSMFGHTF
                     LRLHSTKRNALLDSSFNYAANAEEGNAIIYAWLGMTGGFPGTYAMHPYYVKINEYNDM
                     ESRDLWEFRLRLTKEELEFLQAHLWELSSVYFPYYYLDENCSYQLLTALEAVRPTVTL
                     TNRFSLFVTPTDTLHTLNENGFFTGEASYRAAVFTRYLAFFENSTPQARAVFDELREK
                     RNLPTASSKDETAAVDTLLEYYKYTNDYDMAQWKTADLSHYRELLAWRSGVTEDPGLE
                     QYVVAEADKNPLNGFKSTEVRTGYYSAPAGGAIVAGIRPALREFQDVSLGLSPWSQMQ
                     IMGFSVSYAPLQNLLRLEEFNVVDIVALSPWRAHVQKLSYRLRTGFYRHDQYLAQTSG
                     TLSWDSTAGAGFTLPLGKNAGIFLMAQATGQVSSLWENTVRIAPSALSGIKANWSARL
                     NSVFSYEIDYGLVSNSVALQRFELRNAWAFWKNWVIEADLLYAFARPLAATEDYWRAS
                     VFLKTYF"
     gene            complement(6939..7439)
                     /locus_tag="PLF85_07970"
     CDS             complement(6939..7439)
                     /locus_tag="PLF85_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNE19640.1"
                     /translation="MKKATGISLAVAFAATLAVPASVSAGGYGSAGCGLGAVVFGSKP
                     GMIQVLAATTNATFYSQTFGITTGTSECGGGAIKVEAEQKVYAYNNFTQLQKEIAQGR
                     GERLQTLAYLMGCDSKAVDAFGAAAQKNYSQIFSGKTVDSDMMLERVRSAVQSDAAVA
                     SQCSAL"
     gene            complement(7474..7971)
                     /locus_tag="PLF85_07975"
     CDS             complement(7474..7971)
                     /locus_tag="PLF85_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNE19641.1"
                     /translation="MKKLSLSLAAITAISLAAPMAVSAGGYGAAGCGLGAVVFGSKPG
                     MIQVLAATTNGTFASQTFGITTGTSECGGGVVKVQSEQKIYAYNNFGQLQQEIAQGRG
                     ERLQTLAFMMGCNAKSVDKFGAVAQKNYSQIFADNKTDSELMLERLRSAAQSDATLAS
                     SCSAL"
     gene            8129..>8446
                     /locus_tag="PLF85_07980"
     CDS             8129..>8446
                     /locus_tag="PLF85_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19642.1"
                     /translation="MKLPIVLSPIFLLVFLSACGSSRASYRFNLQQVARGAAFSDESR
                     SIKPPLVFEGADFRLTWSPGPLTFYFRLDNRSKSDIQINYDKSFLLSPRGDVYAPVHP
                     GNSS"
BASE COUNT         1907 a         2288 c         2404 g         1847 t
ORIGIN      
        1 caacatccac ccgtcgctgc tgccggcatt tcctggtctg cgttcgcaga agcaggcgct
       61 cgattatggg gtgaaggtga ctggctgcac gatacacctc gtcgatgctg gcgtcgatac
      121 gggcagaatt ttagcgcagg gagtagtacc ggtgctggcg aaagacagcg aagagacgct
      181 gagccgcagg atactcaagg ccgaacacga cctctactgg cgcaccgtgc agaagcatct
      241 cgaaagatca gctaagaaag cttcttaacg ccgcttcttc tttttcgccg cagggcgtgc
      301 tttgggtttg gctgctttct tcgtcttggt cggtgacttt tttgcaggct ttgttttagt
      361 ggcagcttgg gctgctgcag ccttgtcgtc tttccttaca acaggcttcg gatctttggc
      421 ttcttcggct tcaatgagat cggcgatgca ctctaccccc gccgggccca tgaacaggtt
      481 gcagtgacct acctgtgcca gtttgacatc gtcaaaacga ccgggaatat tcaggtagtt
      541 taaaaacagc acttcgtcaa agcgagcaaa gatcgcctgc acgctcgaga gcgtgcggta
      601 cttgtctttc gtttcgcgca tgaatggcga cagcgggttc atctgcagcg aggcaaaggt
      661 aaacgggaac aggaaggtca gcggatgccc gcggaatggc gtccccagcg tgaagatctt
      721 gcgtgtgcgg tcacgcccgc ggtagcccat gtgggcggct accagtccgc ccatcgaatg
      781 gcagaccagg tagcagtcag agatgctgtt ttcttcgaga aaatgctgca gcttctcgct
      841 gtacttgcgc aggttaccaa actggaagcc caggcgtaca acgtagaccg gatggccctg
      901 cgctgcgagg gccgcacgac acctgagcca ggtgagcggc gagccgaaaa aacccgaaac
      961 gagcacaacc gcgcgcttgt cgccctcggt gatgtcgccc gggcgtatga acaggccggg
     1021 tatgtagagc agcaaatagc ggataccgta gagtaattcg aagataattt gtaccaggag
     1081 gttccgtgaa ggcgtctttt cgggcaagaa ggtcagatgg tctgccattt gttcaccaat
     1141 tccattgccg catactgcgg ccagcagttt ctcttccagg gttttcggga caggtcgtgc
     1201 gacctgcccc gaaaaccctt agcattttgc ttgccagctt tactgtcacc gcttgctaag
     1261 cgcgtgggct gccctcctga tccggtagac gtgtcgatct ggcggtcttc cgaccgtatt
     1321 gcgctgctcg aagagcggta caaccgcttc attgatgaat acgccgaggc tgcgctatta
     1381 gccgggcagg caggggttgg cttcgtcgac ctgctctcca cccacaagaa agagaagttc
     1441 gttcaccgca ccgacgtcag gcctgacgag ttcaaccagt atgccgacga gcaggcacag
     1501 tggcagctgg agtccggtta tagccagcag gttaaagaac tcgaaacggg attgtgtgaa
     1561 ctgcggacgc gtatcctctg ccacctgcgc ggtggcgcgg taacggtgga tggcgacctg
     1621 ctggcgcgcg tgcgcggcgc gcattctaac caccgcgcag aagaccgcca gcaggcgata
     1681 gaatggctga cccggcagtg tgagattctg gcttattata ctgaccccaa ggtcgcgcgc
     1741 aaggcacgca tcgatgctgc ggaatgcagc ctcaggctca accttgcccg gtctgaaata
     1801 tcccgcatgg agcagttacc ggcccatgag ctgctgtatg accgctcgct gacggccatt
     1861 gatacggggc gctttgaaat cccccagcgc tcgcgctgag cagcaggggt acggccctgg
     1921 ggaaacccga acgggccctt taattgacga cctgtctcac cgacaatctg aagagctatg
     1981 gcagacgggg caaaaagcgc gaaagagaca aaaacagccg agaaaaacgg ctcgcaggtg
     2041 atgattgata ccctgctgaa aaacggtatc gacatctgct ggggatatcc cggcggcgca
     2101 attctgcctt tctacgatga gctgtatcat tccaaaatcc gccacatcct ggtgcgccat
     2161 gagcagggcg cagtgcatgc cgcagaaggg tatgcgaagg ccaccggcaa agtcggtatg
     2221 tgtattgcga cctcggggcc gggtgcaacc aacctgatca ccggcatcac cgacgccaag
     2281 atggattctg taccgctgct ggcgctgacc gggcaggtgg cgaccagtgt tatcggcagc
     2341 gatgcgttcc aggaatgcga tacgtatggc atgtcgattc cgattaccaa gtacaacgcg
     2401 ctgctccggt cggccgatga tgtggcgcgc gtgacgcagg aggcgattac cgttgccggc
     2461 gcacgcaggc cagggcccgc gctcattgat tttcctaaag acatccaggt gcagaaaaca
     2521 cgcgtcacga aacctgagat gcggctcgac gcgcggcact acaagaagcc agaaatgcgc
     2581 ggcgacttcg ataagctgat tgcagcgatc agtaaggcgg agcgtccgct gctctatgtt
     2641 ggcggcggtg cgattaccag caatgcgtac aagcagatac gtgagctcgc cgagaaggca
     2701 gatattcccg tctacatgac gctgatgggt ctcggcgcgt ttcccgcgca gcaccggctt
     2761 tcgctcggta tggcgggtat gcacggcaca gcgtacgcca acaaggcaat acaggaatgc
     2821 gacttcatgc tcgcactcgg cgctcgcttc gatgaccgcg tcgcgctcaa tgtcgacgac
     2881 tatggtaagc gcgccgtaaa agccatggtc gacatcgacg aagcagagat caacaagcgc
     2941 gtgccggtcg atatccacat cgtgggtgac ctggttgaag ttttaacgca cctgaacaag
     3001 aatgtgaaga agcagtcgcg caaagcctgg actgatcgta ttgttaagct gaaagaagaa
     3061 aatccgctgc gcttcaatgt cgaacctggt tcgatcaagc cgcagcaggc gattcacgaa
     3121 ctctggaaaa agacgcgtgg tgaagcgatt atctctaccg acgtcggcca gcaccaaatg
     3181 tgggcggcgc agtactatac attcaacgaa ccgcgccagt ggctgacttc gggcgggctc
     3241 ggtaccatgg gctttggttt tcctgcggca ataggagcag cgcttggccg gcctgaaaaa
     3301 gatgtgtttg tcatcagcgg cgacggcagc ttccagatgt gtatacagga actcgctacc
     3361 gtcgcgatgt acaagatacc ggtgaagata ctcctgttta acaacggttt cctcggtatg
     3421 gtaaggcagt ggcaggaact cttctacgac aaccggttct cacagtcaga catgaactac
     3481 aaccccgact tcgtcaaaat ctgctcgggc tatgatattc ctgcaaagcg catcgacaaa
     3541 cagtcggagc tctctgcggg cattgacttt ctcgtgaata gcaagacatc cgcattgctt
     3601 gaagtgacaa ttcccgaaaa agaaaaggtc tacccaatga tcgcttcggg cgcgcgctac
     3661 gagcagatgg tcgattttgt cagtaagaaa gagcagggca ctgaaatgaa agtgcacctg
     3721 aaccggccgg aaaaataggt tgttcgggtt ttgcaggccg ccgcaaaagc ggccagtgat
     3781 ttttgcagcg ctgtttgcag ttgcattacc attgactccg gtcgccgcag aaggcctgcc
     3841 actgctacgc agcatcttcg gcaatgcaac gctgggagaa gcgcgctttc gtgagacaat
     3901 caacaagctg aaccgctacg gggaatacgc attcgccgag gcctaccgca gcgaacaaac
     3961 cctcgcgttc tattcgggaa aagggaacac ctcgtgctgg ctcaagcacc acgacagcga
     4021 cgactacgcc gacaagctga cgcaggaaat cgccgaacac aagtacccgg tattccgtta
     4081 cggcaactgg ctgggctact gcacggccgg cgatacggaa aaacttgcct cgcaggtgcg
     4141 ttacattctc atcggcgccg gcgaatggcg tgagtaagcc ttaaaagttt tcgttatttc
     4201 tcagccagct tttgcagcag ttctgcgcgc aggcggccat gttcgatatg ccaggtgtcg
     4261 aggtggcgac cgcccttaat gcgccacagc ggttgctgca tcgtccgtga tatcttttct
     4321 ccgtggctga tgggcacgac cggatccgcc tcgccgtgga tcaggataac acgtttgcct
     4381 gcgaaagctg atgcgtcgtc gtcgggggcg tgcgtatcgg tgacaagcac ccaggccagc
     4441 ggctgcagcg gccacagaaa ccagcgtgag gcaagcgcag tgcgcgcgac cgcctggtac
     4501 gagaggaaag tcccttcaaa aatgtaagtc tcatcttcgc gtagcccttc gtcgataagg
     4561 gttcgcatga gcactgcgcc gccgaggctc tgcccgtaaa aaatgcggcg ttcattgctg
     4621 tactgccggc gcacttcgcg gacaaatgcc tgcgcatcga gcctgagccc gtcacgcgag
     4681 ctttcacccg ttgattcacc gtagccacga taatcgaaag ttgccagtga atatccttct
     4741 tctatgagcc agctgagaaa ccggtagtgc gcgttcatat tctgcgcatt gccatgaaac
     4801 tggataatga tgccgcggtt ggtttttcct ttggccgggc aaaacatcgt catgaggctt
     4861 gtaccgtcgg ctgtagcgat tggccggcga gagcagttga tgttaaaatc ttcgggcggg
     4921 taccagcgct tctgatcgcc ctgatagaat actgaattgc acgcgataaa aaacagtggc
     4981 aaaagtgcag caatctgtcg catgtcagaa gtacgtcttc aaaaagaccg aagcacgcca
     5041 gtagtcctca gtcgcagcaa ggggcctcgc gaacgcgtag agcaggtcag cttcgatcac
     5101 ccagttcttc cagaaggccc acgcgttgcg cagttcaaac cgttgcagtg ccacgctgtt
     5161 ggagacgagg ccataatcaa tttcataact gaaaactgag ttcagccggg ccgaccagtt
     5221 tgccttgata ccgctcaggg cagacggagc tatacgcacc gtgttctccc agagtgagct
     5281 tacctgcccg gtggcctgcg ccattaggaa aataccggcg ttcttgccca ggggcagggt
     5341 aaagcctgcc ccggccgtgg aatcccatga cagggtgccg gaagtctgtg cgagatactg
     5401 gtcatggcgg tagaaaccgg tacgcagccg gtagctgagt ttctgcacat gcgcgcgcca
     5461 gggcgacaac gccacgatat cgaccacgtt aaattcttcc agccggagca gattctgtag
     5521 cggcgcatag gacaccgaga aacccatgat ctgcatctgc gaccagggtg acaggccaag
     5581 cgatacatcc tggaactcgc gtaatgccgg tcggataccg gcaacgatcg cgccgcctgc
     5641 aggtgcactg taataccccg tacgtacttc ggtagatttg aaaccgttca gcggattttt
     5701 atctgcttcg gccacaacat actgttccag gcccggatct tccgtgacgc ctgaacgcca
     5761 ggcgagtaat tcgcggtagt gcgacaggtc ggcagtcttc cactgtgcca tgtcatagtc
     5821 gttcgtgtac ttgtagtact cgagcagggt atccactgcc gctgtctcgt ctttactgct
     5881 cgcggtcggc aggttgcgct tctcgcgcag ttcgtcgaag acagcgcggg cctgcggcgt
     5941 cgagttttcg aagaaggcca ggtagcgcgt aaagaccgcg gcgcggtaac ttgcctcgcc
     6001 ggtaaaaaag ccgttctcgt ttaaagtgtg cagcgtatcg gtcggcgtta cgaaaagtga
     6061 gaagcggttg gttagtgtca ccgtcggcct gacagcttcc agagctgtca gcagctggta
     6121 agaacaattt tcgtcgaggt agtagtaggg aaaataaacc gagctgagct cccagaggtg
     6181 cgcctgcaga aactcgagct cttctttggt cagccggagc ctgaattccc acaggtcacg
     6241 gctttccata tcgttgtatt cattaatctt aacgtagtag ggatgcatgg cataggtgcc
     6301 gggaaaacca ccggtcatac cgagccacgc gtaaataatg gcattacctt cttcggcatt
     6361 tgcggcatag ttaaaagaag aatcgagcag cgcgttgcgc ttcgtgctgt gcagccgcaa
     6421 aaatgtatgc ccaaacatag aagagggatt tcccatgaaa aagctggcaa agacgatgct
     6481 ggctgaatct gccgatatgt gttctttcca ttcacggtat tttttacagt cgacgacagg
     6541 aagctttttt gtgtcgattt tgagttcgcg caccaggaaa atctcccgcg cgggataccg
     6601 gcagcggccg ggttctgcac tgtaattgtc ggtaaatagg gcgagcagcg ttgcttcgag
     6661 ttcagcttca gggtctcttt cgcccttggc tgaggcaaaa aactcccccg gtgtcttgtc
     6721 acgggcttcg cttctgaggc tgcccaggaa actatcgtag tggatcaggc gtcgccagcc
     6781 tgcagtatct gcgagcttca gctgccgcgc attggtgtag tattgctgtg ccgcgagctc
     6841 gcggagtgtc tgttgtatcg ggtgttcggc tgaaaggcct gccgtggcaa aaaatgccac
     6901 ggcgaggccg gcttgaatta atcgacccgg tgcgactctt agagagccga gcactgagaa
     6961 gcaaccgccg cgtcagactg aacagctgaa cgcacgcgct caagcatcat gtctgaatcg
     7021 acagttttgc ctgagaaaat ctgagagtag tttttctggg cagctgcgcc gaaagcgtca
     7081 acagcctttg aatcgcaacc catcaggtat gccagagtct gcagcctttc gccgcggccc
     7141 tgtgcaattt ctttctggag ctgtgtgaag ttgttgtaag cataaacttt ctgttctgct
     7201 tcaaccttga ttgcgccacc gccacactca gatgtaccgg tagtgatacc gaatgtctgg
     7261 ctgtagaatg tagcgttggt tgttgctgcg agcacctgaa tcatgcctgg cttagaacca
     7321 aatacgaccg cgcccagacc gcaaccagca gaaccatagc cgccagctga aactgaagca
     7381 ggaaccgcga gtgtcgctgc gaaagcaacc gcgagtgaaa tacctgtagc ttttttcatg
     7441 agttatacct ctgtgtcctt atatatccgg cgatcagagc gctgagcacg atgaagccag
     7501 tgttgcgtca gactgagcag ctgaacgcag gcgctcgagc atcagttctg aatcggtttt
     7561 gttgtcagcg aagatttgtg agtagttttt ctgtgctacc gcaccgaatt tgtcgactga
     7621 tttcgcattg cagcccatca tgaaagcgag ggtttgcagt ctttcgccgc gaccctgagc
     7681 aatttcctgc tgcagctggc cgaagttgtt gtacgcgtaa attttctgct ctgactgaac
     7741 tttaaccacg ccgccgccgc attcagatgt gccggtagtg ataccgaaag tctgtgatgc
     7801 gaacgtgccg ttcgttgttg cagccagcac ctggatcata ccaggctttg agccgaagac
     7861 taccgcaccg agaccgcaac cagcagcacc atagccaccg gctgaaacag ccatcggagc
     7921 agcaagagag attgcggtaa ttgccgcaag agagagtgag agttttttca tcttgtttcc
     7981 tccaaagatc agggaatacc ctgccgcggg cacggtgcgg caatcagctg tttcgccaag
     8041 cacgaaaaaa aacaatcagg tacttcatca tgtacatttt tacccggcga gctattgtca
     8101 ggttgagcgg gatttcagac tgtgctacat gaagctaccg atcgtgttgt ctcccatatt
     8161 cctgcttgta tttctcagtg cctgcggctc ttcgcgggcc agctaccgtt tcaacctgca
     8221 acaggttgcc aggggggcgg ccttcagcga cgaatcacgc agcattaagc cacctttggt
     8281 ttttgaaggt gcggattttc gtctgacctg gtcgccggga ccgctcacgt tttatttccg
     8341 cctcgacaat cgcagtaagt ctgatatcca gattaactat gataagagtt ttctactcag
     8401 cccgcgtggt gatgtctatg cgccagtgca tccgggcaac agctct
//
LOCUS       DAOQJF010000124         8413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4560_length_8413_cov_5.454894, whole genome shotgun sequence.
ACCESSION   DAOQJF010000124 DAOQJF010000000
VERSION     DAOQJF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4560_length_8413_cov_5.454894"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..477
                     /locus_tag="PLF85_07985"
     CDS             76..477
                     /locus_tag="PLF85_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19643.1"
                     /translation="MADSRIPEIPLPRPFLRVISEAPVTKEDVERVRKTLQQNIDVLQ
                     AELRREKELTQKLQKSGEVLQEILSAPDFRALSLQSREAVVVTDASKPGAAHVDEPPV
                     EYVEVEEIELTDHEVSSLEGGADPKKKKAED"
     gene            490..1200
                     /locus_tag="PLF85_07990"
     CDS             490..1200
                     /locus_tag="PLF85_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19644.1"
                     /translation="MRYALALLFSCAALIAQDPTQLTPVDEIPDDGYLAIEGGLGYNT
                     YPTSEYFRAPLFFTGASAYRNISTRVLLLSDMVHASDYFAGKQTAVTGSGLYAYNFAL
                     LDVDYLSWQGKVWAAGFGGGLSHQGFLIPGAERSAHAVVGRLRGQFYLFWAEFLATQA
                     VVTLPIAFYQGSTDSFRMLQGEFNILLDFTGHVRNPESQTFLFALSLHYDYTRLTHSV
                     RSYEQHEFTPMIKAMVVY"
     gene            1200..1862
                     /gene="pyrF"
                     /locus_tag="PLF85_07995"
     CDS             1200..1862
                     /gene="pyrF"
                     /locus_tag="PLF85_07995"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNE19645.1"
                     /translation="MQATDKLIVALDVPTAADALGLVGVLGDTVSFYKVGMQLYFAEG
                     NALVKELIARRKRVFLDLKINDIPETVALAVGSLARLGVDYLTLFTNEKQIAAARKVV
                     DDLQSALKLLNVTVLTSDASGVDAVERRAQLSLEAGADGLICSGQETAALRTRHGAGP
                     IIVNPGIRPAGGGADDQVRVVTPAMAIRAGATNIVVGRPITKAKDPRAAAEAIVAEIR
                     GA"
     gene            1873..2826
                     /locus_tag="PLF85_08000"
     CDS             1873..2826
                     /locus_tag="PLF85_08000"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNE19646.1"
                     /translation="MKSLFDKMGPSKLVLSPMAGYSDSPYRKLCREMGSAISISEFVS
                     TEHLFRSHPGAIKLFRYEEPERPVVFQVFGNDPEIIVRGVSRVLHLNPDGIDLNMGCS
                     VKNVSGRGSGAGLLRCPDKSRTIISRLVAETGLPISAKIRLGWDHDSLNYMEFSEMLE
                     SEGVWTIAVHGRTRQMAYTGSADWERIGEIARARKIPIFGNGDVKTPAEAYERIRNYG
                     VAGVYIGRAAIGNPWIFSGIDKATLSYTERLPVMLRHLDDMAKFYGDALAAVLMRKHL
                     TQYTRDLPVYSFLKPEMYRKTSAVELKDFLLRAAEHSALVA"
     gene            complement(2870..4900)
                     /locus_tag="PLF85_08005"
     CDS             complement(2870..4900)
                     /locus_tag="PLF85_08005"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNE19647.1"
                     /translation="MPSLLRSQAPAEGQSLNTLLARMPSYVTRAQKALVKKAYLLAED
                     AHKFQKRLSGEPYIIHPLAVAHTLADLGLDHEVICAGLLHDVLEDTSISPEYMRQEFG
                     ETITYLVESVTKISVLKTQSAENKQAINIRKMIMATIRDSRVILIKLADKLHNMRTLG
                     FHSTSKGMQIAREVLEIYAPLAGRLGIYKIKSELEDLALYHLDRTAYAHIKDKIAEKK
                     GQRDGRVAMVMSLLSQHLKDSKIKATVEGRSKHFFSIYNKMQEQKKSFEDIYDLTGVR
                     VLVNEVQDCYTVLGLVHTLWTPVPGRFKDYISVPKSNGYQSLHTTVIGPGGQAIEVQI
                     RTWKMHRVSEFGIASHWAYKEGQAPTKAFEMQFMELQDMAASEEESDSGEFYQELVSN
                     LSQSSEVYVISPKGKIFTMPEHSTVLDFAFRIHTDIGLRCAGAKIGDRLVSIRTPLKN
                     GDQIEIITNTAVKPSLNWLNFLKTPNARAKLKAHFRKQESPALEKLPETEKKPAGEAR
                     PPRAPKSDVAPTVAPQPHERKIEWQGETQLEVKYAQCCNPMPPDSIAGFISRFGVISV
                     HKKGSPSLMDLSVHPDQAERLVPLKWVGESTGRHASIEIRGIDRPQIYLDIVKQLAQS
                     GANIIEANATTSQVGQIRDKFVIEVDSEDHLQNILKEIMTVDGVEEAVEVAI"
     gene            5117..6622
                     /locus_tag="PLF85_08010"
     CDS             5117..6622
                     /locus_tag="PLF85_08010"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNE19648.1"
                     /translation="MAWLLNLRKSNPFHIVQKLMDEGKYDDAVLKLTAMSEDVDLAPR
                     SYIYLAECHEQLGSKELARECYRKAIDAGAFDDRDREIEIYKKIAEIYRAEGDNDGFF
                     ECCLEILRLNPADELANQEVGLMALGDGHFRIAETYLRAAMQASDEPQLPLAYAVTLW
                     QLGERDNAIAQMEKLLSDANADSNAELLYVTMATYGIHLARGRQAALKLISQSKDSNL
                     LQLLLNIYLYQCYQSKALREALDFLKKLNESGGLPEDKKREYQYLLLLLYLSEEMFLE
                     ASRLYRDVAGDDDEYRDLKHLKVFIDQIDLNPHAENLRPFKQIFKDNFDPLLQPDLAY
                     AISGFRKNRGISMDKFFDLSGEIPALRIEYDVMTAEKGNDLFLQMMPEDFQKFVIYII
                     TKLEYNEPVKESSGEKDLVLYSAISSKSKSIRALFAFYRLRTGAHISDISVRNLQNKM
                     QTLKADKTYIISSAQLTEGALTVLRNEPSLKQFDGEHLAEWLHDYYKAARR"
     gene            complement(6623..7279)
                     /locus_tag="PLF85_08015"
     CDS             complement(6623..7279)
                     /locus_tag="PLF85_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HNE19649.1"
                     /translation="MSRIKGSWINFWKIPDQRYEFIFAAVILTVVMLSSVPFLTQIEM
                     RPGVQLHDPVFNYLPAHDLSDLIFYILYTALLLGVIFLLPSPERLVIGFEAYALFVVL
                     RMITMTLVPLEPPNGLIPLVDPILSFMYTGKQVNKDLFFSGHTATMYLLYLMLPAGIY
                     RKLAFVGVIVMGICLLFQRVHYTVDVIVAPFFAYGALRIVLRLRRTIGLKASGFVAGE
                     "
     gene            7389..7661
                     /locus_tag="PLF85_08020"
     CDS             7389..7661
                     /locus_tag="PLF85_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNE19650.1"
                     /translation="MFWQTFITVFTTVFLAEIGDKTQLATMLFAANKEVNLMHVFAGS
                     AVALVVASALGVFAGAFLGTYIPTKILHYAAGLGFVVIGVWTIYRA"
     gene            complement(7695..>8413)
                     /locus_tag="PLF85_08025"
     CDS             complement(7695..>8413)
                     /locus_tag="PLF85_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE19651.1"
                     /translation="REIRFTDVSFGYRENQSVLRDISLVLKPSRIYAIVGESGIGKTT
                     LIDLLCRFYQPQLGHISLNGVQSDRYDLAAWRAGISIVTQDAILFDGSVRENLLISSR
                     PLSDAQLLRALRGADAQDFIDELPQGLDTLIGERGFRLSGGQKHRLTLARALVKGAPV
                     LVLDEATAHLDSLSEQKIRVQLDKLKKNRVVIMITHRLSTVTHADEIIVLNGGSVEAI
                     GTHRDLLRRSRTYKRLWSAQ"
BASE COUNT         1831 a         2358 c         2395 g         1829 t
ORIGIN      
        1 ggccgcttcg tccgccccga acgttgcgga tgacgacgcc agaaatgccg cggtgcggtg
       61 tcgcacgagg tacccatggc tgattcacgc ataccagaaa ttcctctgcc ccggcccttc
      121 ctgcgtgtga tcagcgaagc acccgtcacg aaagaagatg tcgagcgcgt gcgtaagacc
      181 ctgcagcaga acatcgatgt tttgcaggca gaactcaggc gcgaaaaaga gctcacccag
      241 aagttacaga aatctggtga agtattgcag gagattcttt cggctccgga ctttcgcgcc
      301 ctcagcctgc agtctcgcga agcggtagtg gtgacagacg ccagcaagcc cggtgcggcg
      361 cacgtcgatg aaccgcctgt agagtatgtc gaggtcgaag agattgagct gacagaccat
      421 gaagtcagca gtctagaagg tggggctgat cctaaaaaga aaaaggccga ggactgattg
      481 atgcagttcg tgcgttacgc actggcactt cttttctcct gcgcagcgct gatcgcgcag
      541 gacccgacac agctcacgcc ggtagatgaa atacccgatg acggctacct cgccatcgaa
      601 ggtgggctcg gctacaacac gtacccaacc tcagaatact tccgcgcgcc gctgtttttt
      661 accggcgctt cagcctaccg taacatcagc acgcgagttc tgttgctctc ggacatggta
      721 cacgcgagcg actatttcgc cggtaagcag acagcggtta caggcagcgg cctctacgcg
      781 tataattttg cgctactcga tgtcgactac ctgtcgtggc agggtaaggt ctgggctgcg
      841 ggatttggcg gtggcttaag ccaccagggg tttttgatac ccggagcgga gcgcagcgca
      901 cacgcggtgg tcggtcgcct gcgcggacag ttctacctct tctgggcgga gtttctagcc
      961 acgcaggcag tggtcaccct gccgatcgcc ttctaccagg gttcaaccga ttcgtttcgc
     1021 atgctgcagg gtgagttcaa catactgctc gattttaccg gacacgtgcg caaccccgag
     1081 tcgcagactt tcttgtttgc cctttcgctt cactacgatt acactcggct cacccacagc
     1141 gtgcgcagct atgagcagca cgagttcact cccatgatta aggcaatggt ggtatactga
     1201 tgcaggcaac agacaaactg atcgtggcgc tcgacgtgcc cacagcggca gatgcgctgg
     1261 gcttagtggg agttctgggc gacacggtgt cgttctacaa ggtgggcatg cagctctact
     1321 ttgccgaggg caatgcactg gtgaaagaac tcatcgcccg cagaaagcgg gtctttctcg
     1381 atctgaagat caatgatatt cccgagacgg tggcgctcgc tgtcggttcg cttgcccggc
     1441 tgggcgtcga ctacctgaca ctcttcacta atgagaaaca gattgctgcg gcgcgcaagg
     1501 tcgtcgacga tctgcagtct gccctgaaac tgttgaatgt cacggtgctg accagcgatg
     1561 cgagcggtgt cgatgctgtg gagcgccgcg cgcagctgtc gctcgaagcc ggcgccgacg
     1621 ggctgatctg ttccgggcag gaaactgcag cactgcgaac caggcacggc gccggaccca
     1681 tcatcgtgaa cccaggcatc aggccagcag gtgggggggc ggacgaccag gtgcgcgtcg
     1741 ttaccccggc catggcaatt cgggcaggtg caaccaacat cgtggttggc cggcccatta
     1801 ccaaggcgaa agatccgcgc gcggcggcag aagcgatcgt cgctgaaata cggggagctt
     1861 gaggcagcag gcatgaaatc cctcttcgac aaaatgggcc cctcgaagct ggtgctctca
     1921 ccgatggccg gctattccga ttcaccgtac cgcaaacttt gccgcgaaat gggttcggcg
     1981 atcagcatca gcgagtttgt ttcgacggag cacctgttcc gttcgcaccc gggcgcgatc
     2041 aaactgtttc gttacgaaga gccagagcgg ccagtcgtgt tccaggtgtt tggcaacgac
     2101 cctgaaatta tcgtgcgcgg ggtctcgcgc gtgctgcacc tgaaccccga cggcattgac
     2161 ctcaacatgg gatgttcggt gaagaacgtc tcaggccgtg gttcgggtgc gggacttctg
     2221 cgctgccccg acaaatcgcg cacgattatc agccggctgg tcgccgagac gggactgccg
     2281 atctcggcaa aaattcgcct cggctgggac cacgactcgc tcaactacat ggaattttcc
     2341 gagatgctcg aaagcgaagg tgtctggacc atcgccgtgc acgggcgcac acggcagatg
     2401 gcctataccg gttcagccga ctgggagcgc atcggtgaaa tagcccgcgc acgcaagata
     2461 cccatcttcg gcaacgggga cgtcaagacc ccggctgaag cctatgagcg catcaggaac
     2521 tatggcgtgg ccggcgtcta cattggccgt gccgcaatcg gtaacccctg gattttttct
     2581 ggtatcgata aggcaaccct gagctacaca gagcgcctgc ctgtgatgct gcggcacctc
     2641 gacgacatgg cgaagttcta cggcgatgca ctcgccgcgg tactgatgcg caagcacctg
     2701 acacagtaca cgcgcgacct gccggtttac tcgtttctca aacccgagat gtaccgcaag
     2761 acatcggcag tcgagctcaa agactttttg ctgcgcgccg cagagcacag cgctctggta
     2821 gcttgattgc tgcgcgatag gtcgcctgag cgacctgccg cgcagtcctt taaatcgcta
     2881 cctctaccgc ttcttctacc ccgtcgacgg tcatgatctc tttcagtatg ttctgcaggt
     2941 gatcttctga atcgacttca atcacgaact tgtcgcgaat ctgcccgacc tgcgaggtcg
     3001 tcgcattcgc ttcgatgatg ttcgccccgc tctgtgccag ctgcttcaca atgtcgaggt
     3061 agatctgcgg acggtcaatg ccgcgtattt cgatcgaagc atgacgcccc gtgctctcgc
     3121 cgacccactt cagcggcacc agccgctcgg cctggtcggg gtgtacgctg agatccatca
     3181 gcgacgggct ccctttcttg tgcaccgaga tcactccaaa gcgcgaaata aaaccagcga
     3241 tgctgtcagg gggcatcggg ttacagcact gcgcgtactt gacctcgagc tgcgtttcgc
     3301 cctgccattc gatcttgcgc tcatgtggct gcggtgcaac agtgggagcg acatcacttt
     3361 tgggggcacg cggcggccgg gcttcgcccg caggtttttt ttccgtttca ggtagttttt
     3421 cgagcgcagg actttcctgc ttccggaaat gcgccttgag ctttgcgcgc gcattgggag
     3481 ttttcaggaa gttgagccag ttgagcgacg gtttgacggc cgtgtttgtg ataatttcaa
     3541 tctggtcgcc gttcttgagc ggggtcctga tactcacgag gcgatcgccg atcttggcgc
     3601 ccgcgcagcg caggccgata tcggtgtgga tacgaaaggc aaaatcgagc accgttgagt
     3661 gctcgggcat cgtgaaaatt tttcccttcg gcgagatgac gtagacctcg ctgctttggc
     3721 tgaggttaga gacaagctcc tggtaaaact cgcccgagtc agattcttct tctgacgctg
     3781 ccatgtcctg cagttccatg aactgcattt caaatgcctt tgtcggcgcc tggccttctt
     3841 tataggccca gtgagatgca ataccgaatt cagaaacgcg gtgcattttc caggtgcgta
     3901 tctgcacctc gatcgcctgt ccgccggggc cgatcacggt ggtgtgcagg ctctggtaac
     3961 cgttggattt aggtaccgag atgtagtcct tgaagcgccc gggtaccggt gtccagagcg
     4021 tgtgcacgag tcccagtacc gtgtagcagt cctgtacttc gttgacgagt acgcgcacgc
     4081 ccgtcaggtc gtagatatct tcaaagctct tcttctgctc ctgcatcttg ttgtagatag
     4141 agaagaagtg ctttgaacgt ccctcaactg ttgccttgat ttttgaatct ttcaggtgct
     4201 gcgacagcag cgacatgacc atcgctacgc gaccgtcgcg ctggcctttc ttttcggcaa
     4261 ttttgtcttt aatatgcgcg taggcagtcc ggtcgaggtg gtagagcgcg aggtcttcga
     4321 gctccgactt gatcttgtag atacctagcc tgccggcaag gggcgcataa atttcgagta
     4381 cctcgcgcgc aatctgcatg cccttcgagg tcgagtggaa gccgagggtg cgcatgttgt
     4441 gcagcttatc ggcgagtttg atcaggataa cccggctgtc gcggatcgtt gccatgatca
     4501 tcttgcggat attgatcgcc tgcttgttct cggcactctg cgtcttgagc acagaaattt
     4561 tcgtcaccga ttcgaccagg taggtaatag tttcgccgaa ctcctgccgc atgtactccg
     4621 ggctgatgga ggtgtcttcg agcacatcgt gcagcagacc cgcacagata acctcgtggt
     4681 cgagccccag gtcggccagt gtgtgcgcca cggcgagcgg atggataatg taaggttcgc
     4741 ccgaaagccg cttctggaac ttgtgcgcat cttcggccag caggtatgcc tttttgacca
     4801 atgccttctg tgcacgggtc acgtacgacg gcatgcgcgc cagaagcgtg ttcaggctct
     4861 ggccctcggc gggggcctgg ctgcggagta acgaaggcat cgccatataa gttattatac
     4921 tcccgcgggg tttagcgcgc aacaaaaatt ctggcgccaa aagaccagac tgacgggcct
     4981 tgagccctct tatgctttac ctgccagcga ttccgccgat actaaagaca gtgcccggcg
     5041 ggacctgatg ccgggccatg gaaccgcgca gatgctatac tttgccctac tgagtgttat
     5101 tctactgggc gcgctattgg cgtggttact gaacctgcgc aaaagtaacc ctttccatat
     5161 cgtgcagaag ctgatggacg agggcaagta cgatgatgca gtcctgaagt tgaccgcgat
     5221 gtccgaggat gttgatcttg cgccacggtc ttatatctac ctcgccgaat gccatgagca
     5281 acttggctcg aaagaactgg cccgcgagtg ctaccgcaag gcaatcgatg ccggggcttt
     5341 tgacgaccgc gatcgcgaaa tcgaaatcta caaaaagatt gcagagatct accgcgccga
     5401 aggcgacaat gacgggtttt tcgagtgctg tttggagata ctgcgattga accccgccga
     5461 cgaactggca aatcaggaag tgggattgat ggcgctcggc gacggtcatt tccgcattgc
     5521 agagacctac ctgcgcgcgg cgatgcaagc ctcagacgaa ccgcagctgc cacttgcata
     5581 tgccgtgacg ctctggcagc tcggcgaacg ggataacgcg atcgcgcaga tggaaaaact
     5641 gctctctgac gctaacgccg acagcaatgc agagctgctc tacgtgacga tggcgaccta
     5701 cggcatacac ctggcgcgcg gcaggcaagc tgcgctcaag ctgatcagcc agagcaaaga
     5761 cagtaatctg ctgcagctgc tgctcaatat ctacctgtac caatgctacc agtcgaaggc
     5821 tctgcgcgaa gcgctcgact tcctgaagaa gctgaacgaa tcgggtggcc tgcccgagga
     5881 caagaagcgg gagtaccagt acctgctgct gttgctatac cttagcgaag agatgttcct
     5941 cgaagcctcg cggctttacc gcgacgttgc aggtgacgac gatgaataca gggacctgaa
     6001 gcacctcaag gtattcatcg accagatcga tctcaatccg catgccgaga acctcaggcc
     6061 attcaaacag atattcaagg acaactttga tccgttgctg cagcccgacc tggcgtacgc
     6121 catttcgggc tttcgtaaga accgcggtat ctcaatggac aagttctttg atctgtcggg
     6181 cgaaattcct gcgctgcgca ttgaatatga tgtcatgacc gctgagaagg gaaacgacct
     6241 ctttctgcag atgatgcccg aggatttcca gaaatttgtt atctacatta ttaccaaact
     6301 cgaatacaat gaaccggtaa aagaatcttc gggcgaaaag gacctggtgc tctattcggc
     6361 aatctcatcg aagagtaagt cgattcgggc gcttttcgcc ttttaccgcc tgcgcaccgg
     6421 agcccatatc agcgacatct cggtgcgcaa cctgcagaac aagatgcaga cgctcaaagc
     6481 agataaaact tatattatct caagcgcgca actcaccgaa ggagcgctca ccgtacttcg
     6541 caacgaacct tctttgaaac agtttgacgg cgaacacctt gccgaatggc tgcacgacta
     6601 ctacaaggct gcgcgacggt aattactcgc ccgcaacaaa accgctggcc ttgagcccga
     6661 tggtccggcg cagccgcagg acgatgcgca gcgcaccgta agcaaagaac ggcgcgacga
     6721 tcacatcgac agtgtagtgc acccgctgga acagcaggca gatacccata acaatcacgc
     6781 cgacaaacgc gagcttgcgg tagatacccg ccggcagcat caggtaaagc aggtacatcg
     6841 tcgctgtgtg ccccgaaaaa aagaggtctt tgttgacctg cttacccgtg tacatgaagc
     6901 tcaggatagg gtcgaccagt ggaataagcc cgttcggtgg ctcgagcgga accagcgtca
     6961 tggtaatcat gcgcagaacg acaaaaagtg cgtaggcctc aaagccaatc acgagccgct
     7021 caggcgaagg cagcaggaaa atgaccccca gcagcagcgc cgtatacaag atgtagaaaa
     7081 tcaggtcact caggtcgtgc gccggcaggt agttaaaaac cgggtcgtgc agctgcaccc
     7141 ccggacgcat ctcaatctgc gtgagaaatg gtacagagct cagcatcacg acagtcagta
     7201 taactgccgc gaagatgaat tcataacgct ggtcaggaat tttccaaaag ttgatccagc
     7261 tgcctttgat acgcgacata ataaatcaga tgcccaccaa gactggatgt gtcaggcagg
     7321 gatctttttc tgcgtcagca agcgaaaaaa ggatttgcag cagcacggca gagagggttg
     7381 aagagagcat gttttggcaa actttcatca ctgtgtttac cacggttttt ctcgcagaaa
     7441 ttggcgacaa aacgcagtta gcaaccatgc tgttcgcagc gaataaagag gttaacctca
     7501 tgcacgtttt tgcagggtcg gcagtagcgc tggtggtcgc ttctgcgctg ggagtatttg
     7561 ccggcgcttt tctgggaacc tacattccaa ccaaaattct gcattatgcg gcgggactcg
     7621 gctttgtcgt tatcggcgtc tggacgattt accgcgccta gcaggttcct gtatcaggag
     7681 cttgttaggg cattttactg cgcgctccac agcctcttgt aggtacgcga gcggcgcagc
     7741 aggtcgcggt gcgttccgat ggcttcaacg gagccgccgt tcaggacgat aatttcatcg
     7801 gcgtgcgtga ctgtgctgag gcggtgcgta atcataatca cgacacgatt tttcttgagt
     7861 ttatccagct gaaccctgat cttctgctcg ctgagcgagt cgaggtgagc ggtagcctca
     7921 tccagcacca gtaccggtgc acccttcacc aatgcgcgcg ccagggtcag gcgatgtttc
     7981 tgcccacctg aaagcctgaa accgcgttcg ccgatcagtg tgtctaaccc ttgtggtaat
     8041 tcgtcgataa aatcctgcgc gtcggcgccg cgcagggccc gcagaagttg cgcatcgctc
     8101 agcggccggg aggagatcaa caggttctca cgcacagacc cgtcgaacag tatggcatcc
     8161 tgcgtgacga tcgagattcc cgcacgccag gccgcgagat cataacgatc gctctgtacc
     8221 ccgttgaggc tgatgtgtcc caattgtggc tggtagaagc ggcagagcag gtcaatcagc
     8281 gtcgtcttac cgataccgct ttcgccgacg atcgcgtaga tgcgcgaagg ttttagcaca
     8341 agcgaaatgt cgcgcaatac cgactggttt tcccggtagc caaaggacac gtctgtgaac
     8401 cggatttcgc gca
//
LOCUS       DAOQJF010000125         8361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4601_length_8361_cov_5.550084, whole genome shotgun sequence.
ACCESSION   DAOQJF010000125 DAOQJF010000000
VERSION     DAOQJF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_8361_cov_5.550084"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1737
                     /locus_tag="PLF85_08030"
     CDS             163..1737
                     /locus_tag="PLF85_08030"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNE19652.1"
                     /translation="MSKLIPGLTMIPLALFCNLASAQDVLGKSQDPAATVSNAAAPKT
                     PAIKTVTNFSQALRCMDELFLAYGKNGIVVTSTGIPDETGKVRTGTKEMLITAIAKMT
                     VKSNAFEFIDFHGGGDDLARLFDTKGQSMMKIPDYYIRGSITQMDDNTIRKNSGFGLA
                     AAFFDFGFSSDASYDLISMDMSIGDAATRKILPQTSTSNTMVIIKTGKSGEAGGKLGK
                     VGLSFNLDFSRSEGLGATTRTLLELGLIETLGQFTQVPYWRCLDADITNPVIREQALE
                     TYETLKENERITFVQRKLGGSMNRYNGPIDGKMNDTLKDAILEYQAQNNLVADGRISF
                     DLYASLLDDIQNNLAYVPSTPPAKTAAARPIPAPAPAPAPVALPPPQAVPAAAFRVNL
                     DSDKGSKPSYKVGEFLNMSLSLTGDGTVYCYYEDINRSVARIFPNQFFGNPTLRSGST
                     VRLPSGGFRIRFDQPGKERVACIGADRELVVPGNLKGARDLAPLPVRSLDEVVSQYRQ
                     GNPTAMSSYVDITVTR"
     gene            1752..2336
                     /locus_tag="PLF85_08035"
     CDS             1752..2336
                     /locus_tag="PLF85_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19653.1"
                     /translation="MSSLVNRLLLNSALILPILVPMAMASESQAEKAWLLAELIIGPE
                     KTPSKSTSQSQREKAVRYQGEAPANPEDEGVGILAPRGGAPIEEKAFENRNKARAYQS
                     GSDNPTPPPSSSGVAGFDGGQPRQTEQRNRARAYTGTGNAQDIDLSHVGRDGIPLVTC
                     HDVDNVSGRIGDDTISGSIVHIFRNGQQVKVRCR"
     gene            2406..2717
                     /locus_tag="PLF85_08040"
     CDS             2406..2717
                     /locus_tag="PLF85_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19654.1"
                     /translation="MKWWALLSLAILGVAMVAQAQVSVDVSGLGVKVQTGKGGSVASN
                     TAGTIDPDVQMEGVAVINGEVFVDGEKVPKGKSPYTSRKSGKTYVIKWGKDGNVAIHE
                     K"
     gene            2707..3051
                     /locus_tag="PLF85_08045"
     CDS             2707..3051
                     /locus_tag="PLF85_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19655.1"
                     /translation="MKNSLLGIFTMSLLVSSAQAVIIVDYNAVDYNVVMPPPPGGLTS
                     RFVPLAQTEPGYLMQRSNAWRVYNQDRPRTGALLVYPWVSGSIGAPTSLRQVEVRNNI
                     SRAQAYRLIPRE"
     gene            3181..4071
                     /locus_tag="PLF85_08050"
     CDS             3181..4071
                     /locus_tag="PLF85_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19656.1"
                     /translation="MKSGFDNTAGYIRKLLGASVLMAMLQSAWALDAVQGDFTQVLET
                     RSGELTVWQIADNPDIYIFDFPGLTYQGRSFNRITQFTEQQTTEAYPKVLGNQELARY
                     VEAARRTQADFAFGHDVLISELVQFFNFSLRDKIELNPEEIAIREFLIEQGLARFWRG
                     FYQAMKPGVVVLAIPQTQDRKVGEPMVSPGARYAILLHELAHGEYYTNPHYAKYCQRF
                     WHETLTEYQRELFKRFLMNFNYAVNHEELLINEMQAYLMFTPDPKSFSARKLGVSDAE
                     LQSMRETFRRGSPPIRLPLR"
     gene            4086..4397
                     /locus_tag="PLF85_08055"
     CDS             4086..4397
                     /locus_tag="PLF85_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19657.1"
                     /translation="MKMLGLTIIALTTLTIFAGSSFAQTSQRTKVQGNTEINATTKNM
                     TAVASGDNNVAKNRVGVIQGDQKTSTKVNVAAGNVTTVAIGRNRKACTNIGGVVSDDC
                     K"
     gene            4384..4698
                     /locus_tag="PLF85_08060"
     CDS             4384..4698
                     /locus_tag="PLF85_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19658.1"
                     /translation="MIANKLLMSLAIASAMTFSMLDAYAVQGLDAHGNQKVNSAMAKR
                     WSEQAKGQKTPTDEKQVVNVGSKKQGTCNVNVGTTQPNDNRGGRKNDIVVTTKEVINV
                     CK"
     gene            4706..5317
                     /locus_tag="PLF85_08065"
     CDS             4706..5317
                     /locus_tag="PLF85_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE19659.1"
                     /translation="MNNNISRVLIGLMLLSGVGLSQAASDEEKLRELERAMSMPSETG
                     MPQQKRTRAIVFDNQQQSTSPAAEPAAQVAASTAGSRDCGSLPPDVRTVGVDFAIQFN
                     AGSAMVSAASEGTLNQIAKVLALSPDRCVIVEGHTDVAGNFDKNMALSRDRANAVVNF
                     ISTRNGIDRKRFVPVGKGSTETMPNLDARDPKNRRVVFKVVTG"
     gene            5357..5671
                     /locus_tag="PLF85_08070"
     CDS             5357..5671
                     /locus_tag="PLF85_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19660.1"
                     /translation="MRLISMMIAAAIVASPAFAQQTQRSSNPQGVQIQGNTDIKAKNE
                     NVTAVAVGEGNEAKNTTGAIKGGTQIQGNTKIQASQKNATAVAVGKNNTAANEAGVIG
                     GK"
     gene            5707..6063
                     /locus_tag="PLF85_08075"
     CDS             5707..6063
                     /locus_tag="PLF85_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19661.1"
                     /translation="MEETNMRTQFAIIALASAVISTAAFGQASQTGPKGSMSSPNAQG
                     VQIQGNTDIKAKNENVTAVAVGEGNTAKNTTGAIKGGTQIQGNTKIQASQKNATAVAV
                     GKNNTAANEAGVIGGK"
     gene            complement(6168..8168)
                     /locus_tag="PLF85_08080"
     CDS             complement(6168..8168)
                     /locus_tag="PLF85_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19662.1"
                     /translation="MKQISYTCALLLLMLGLPSIGVAGMFRQYSDALNETVTLVKTGE
                     LKQAVAKIENFNEGNDKDILYFFEKGELLSLGSNFISSRETWMKSDEIIQAWENEFRS
                     NPSKIFGDIGSFLISDKTRRYDGQDYEKVLLSTRLTMNHIMLGNFDHARIEMKKTYER
                     EKLIEAFREKEYDALKVESNKQDAGQASNLEGYPMQELDTPEVRELKNGFQNAFAHYL
                     AGYFFEVTDEPSLAEPGYRNALQLAPGNTMIKTALNEIGKRVPGLGETDVLFVLETGF
                     APRIDSLNISIPIMRKGGIVITPLSFPLLKVSEQVPVPSSLAVAGGRYPVETLTNIDT
                     MARRLLKDQMPGIILRTVIRAGLKSVVQEQANKAHWVAGLVANVVSASTEQADDRNWR
                     TLPERISIARAMLPQGRQVIEFQTPAGIYRTEADVSGRFTIVPIRITGNAVYVGQPNR
                     AKDISPGLLPDKMIPPGHLVPPAPDSSRSYVAAASSVAPVAPVVPVAPVAPAMQPTAD
                     LGKRVQVGDTTYIGDFRFAQPSGLPSGKGVIEWQNGHRFEGQLVNGVKQGHGVFIAPG
                     SFRYEGNWESNKQNGKGKTIFDNGDVYEGEILDGEFHGQGSYTTKDGYRYEGSWKKGV
                     KEGPGKLIFPDGDFWEGIFSNDQRTAQGKMTFAPKPDIRARQ"
BASE COUNT         1986 a         2200 c         2232 g         1943 t
ORIGIN      
        1 gacggtttgc cgagtacgga agacccgtct gggtcgttct gatacaaaca aatcgtgaaa
       61 tatttccttg ttagacggct atctcttggt ggtagagtat gctattggac gcaatttttt
      121 tcgattgcgc tgaacgatcg atttcatcaa gaaggtttaa ctatgtcgaa actgatcccc
      181 ggtttaacga tgattccact ggcgctgttc tgcaaccttg cttctgcgca ggacgtgctc
      241 ggcaaatcgc aagacccggc agcaaccgtt tccaacgctg ccgccccgaa gaccccggcc
      301 atcaagacgg tgaccaattt ttcccaggcc ttgcgatgca tggatgaatt gttcctcgcc
      361 tatggcaaga acggcatcgt cgtgacctcg accggcatcc cggatgagac cggcaaggtg
      421 cgcacaggta ccaaggaaat gctgattacc gccatcgcca aaatgacggt caagagcaac
      481 gcctttgagt ttatcgactt tcatggcggc ggagacgact tggcacgact tttcgacacc
      541 aagggccagt ccatgatgaa aatccccgat tactacattc ggggatcgat cacccagatg
      601 gatgacaata ccattcgcaa gaacagtggt ttcggtttgg cggccgcttt cttcgacttt
      661 ggtttctctt ccgatgcctc ctatgacctg atcagcatgg acatgagtat cggcgatgcg
      721 gccactcgaa agattcttcc gcaaaccagc acttccaata cgatggtcat tatcaaaacc
      781 ggcaaatccg gtgaagcggg aggtaaattg ggtaaggttg gcctctcttt caatctcgac
      841 ttcagccgct cggaaggcct gggtgcgacg acgcggactc tgcttgagct cggcctgatc
      901 gaaacccttg gtcaattcac ccaggtgcct tattggcgtt gcctggatgc ggacatcacc
      961 aatccggtga tccgcgagca ggcactggaa acctacgaaa cgctgaaaga aaacgaaagg
     1021 atcacttttg ttcagcgcaa gctgggtggc agcatgaacc gttacaatgg cccgatcgac
     1081 ggcaagatga atgacacgct gaaggatgcc atccttgaat accaggcaca gaacaatctg
     1141 gtggcggatg ggcgaatcag cttcgatctt tacgccagcc tgctcgatga catccagaac
     1201 aacctggctt atgtgcccag cacgccaccg gctaaaacag cggctgcccg gccgattcct
     1261 gcgcctgcgc ctgcgcctgc gccggtagca cttcccccgc cgcaggctgt ccccgccgct
     1321 gcattccggg tcaatctcga cagcgacaaa ggttccaagc caagctacaa ggtcggagag
     1381 ttcctgaata tgtcgctatc cttgacggga gatggcacgg tatattgcta ctacgaagat
     1441 atcaatcgtt ccgtggcacg gatttttccc aaccagttct ttggcaatcc gaccttgcgc
     1501 tctggcagca ccgtcaggct tcccagcggc gggttccgga ttcgcttcga tcaacctggc
     1561 aaggagcggg ttgcctgtat cggtgctgac cgtgaactgg ttgtcccggg taacctgaaa
     1621 ggtgcccggg atttggctcc tttgcctgtt cgctcgctgg atgaagtcgt gagccagtac
     1681 cgtcaaggta acccgactgc catgagcagt tacgtcgaca tcacggttac ccgctaatcg
     1741 acgagatcgc catgtcctcc ctggtcaaca gacttctcct gaacagtgca ttgatcctcc
     1801 caattctggt acccatggcc atggcgtcag agagccaggc ggagaaggcc tggctgttag
     1861 cagaattgat cattgggccg gagaagaccc cttcgaaatc gacttcgcaa tcgcagcgtg
     1921 agaaggcagt gcgctatcag ggcgaagcac cggcaaaccc ggaggatgag ggagtcggga
     1981 ttcttgctcc tcgtggcggc gcaccaatag aagagaaagc gttcgaaaac cgcaacaagg
     2041 ctagggcata tcaatcaggc agtgacaatc ctacgccacc accatcctct tccggggttg
     2101 ccggattcga tggcggccaa ccgcgccaaa ctgagcaacg caatcgcgca cgtgcctata
     2161 ccggaacggg gaacgctcag gacatcgatt tgagccacgt cggtcgggac ggtattcctc
     2221 tggtgacttg ccatgatgtc gacaatgttt ccgggagaat tggggacgac accatatcag
     2281 gcagcatcgt gcatatcttt cgcaatgggc agcaagtcaa agttcgttgt aggtgaactt
     2341 tcatgtcaac cgaacacttg cccaatgcgt tatacgatta atagatgtga aatatggaga
     2401 tattaatgaa atggtgggcg ctcctttctc ttgccattct cggtgttgca atggttgcac
     2461 aggcgcaagt atctgttgat gtgtccgggc ttggcgtgaa agttcagaca ggaaagggtg
     2521 gttcggttgc cagcaacacg gcgggcacca ttgacccgga tgtacagatg gagggtgttg
     2581 ccgtgattaa tggggaggta tttgtggatg gggaaaaggt cccaaaagga aaatcaccct
     2641 atacctccag gaagtcaggc aaaacctatg taatcaagtg gggaaaagat ggcaatgtcg
     2701 caattcatga aaaatagcct attgggcatt tttaccatga gcttgctggt atcttcggct
     2761 caggcggtaa ttatcgttga ttataatgct gtcgattata atgtcgtgat gccgccacct
     2821 cctggggggt taacgtcacg cttcgttccc cttgcgcaga cagagccggg ctatctcatg
     2881 cagagaagca acgcatggcg tgtttacaac caggaccgtc cacggacagg tgctttgctg
     2941 gtttatccgt gggtatcggg ctcgattggt gcgccgactt ccttgcggca ggtcgaagtt
     3001 cgtaacaata tatcacgtgc ccaggcatat cgcctcatcc cccgtgaata ggttttccaa
     3061 taaacccata gttgctgata gctgacacgc accagaacta cgctacgata atcataccca
     3121 agttgctttg ctttgtactg cgcgggacaa cttctcgaac gatccgaatg gaattgacaa
     3181 gtgaaatctg gatttgacaa cactgccgga tacatcagga aactgctcgg agccagcgtg
     3241 ctcatggcca tgttgcaatc ggcttgggcg ctggacgccg tgcagggcga ctttacgcag
     3301 gttctcgaga cccgcagtgg agagctgaca gtctggcaga tcgccgataa tcccgacatt
     3361 tacatattcg attttccagg tttgacctat cagggccgca gtttcaaccg cattacccaa
     3421 tttactgaac aacagaccac agaggcttat ccaaaggtgc tcggcaatca ggagttggct
     3481 cgctatgttg aggctgcacg ccgcacacag gccgactttg ctttcggtca tgacgtcctg
     3541 atctccgaac tggtgcagtt cttcaatttt tcattacgtg acaagatcga actgaaccct
     3601 gaagaaattg cgattcgcga gttcctgatt gaacagggtc tggcgcgttt ctggcggggt
     3661 ttttaccaag ccatgaaacc gggcgttgtc gtgcttgcca taccgcagac tcaggaccgg
     3721 aaagtgggtg agccgatggt gtcaccaggc gcacgctatg ccattctgtt gcacgaactt
     3781 gcccacggcg agtattatac aaacccgcat tacgccaaat actgccagcg tttctggcac
     3841 gaaaccttga cggaatatca gagggaactt ttcaagcggt ttctcatgaa cttcaattat
     3901 gcggttaatc acgaggaact cctgatcaac gaaatgcagg catatctgat gttcacgccg
     3961 gatcccaaat cattcagtgc tcgcaagctc ggtgtttcag atgcggaatt gcaatcgatg
     4021 cgagaaacat ttcgtcgcgg gagtccgccc atccgattac ccctgcgcta accgggaggc
     4081 atgaaatgaa aatgcttggc ctgaccataa ttgctttgac aactttgacc atcttcgccg
     4141 gttcatcttt cgcgcagacc agtcagcgga ccaaggttca gggcaatact gaaatcaatg
     4201 caaccacaaa aaacatgact gcggttgcct ctggagacaa caacgttgcc aagaatcggg
     4261 ttggtgtgat tcagggtgac cagaagacca gcaccaaagt gaatgtcgct gccggcaacg
     4321 tgacgacggt tgccattggg cgaaacagaa aagcttgtac caatattgga ggagtggtca
     4381 gtgatgattg caaataagct actgatgagt cttgccatcg cctctgcgat gactttcagc
     4441 atgctcgatg cttatgctgt tcagggtctg gacgcgcatg gaaatcaaaa agtcaatagt
     4501 gcgatggcta aacgctggtc tgaacaggcc aaaggccaga agacaccgac tgatgagaag
     4561 caggtcgtca atgtcggcag caagaagcaa ggcacctgta atgtcaatgt cggaacgaca
     4621 caacccaacg ataaccgggg cggtcgaaaa aacgacattg ttgtaacaac caaagaagtc
     4681 atcaacgttt gcaagtgaga tcacaatgaa taacaacatt tccagggttt tgatcggctt
     4741 gatgttgctc tccggcgtgg gtctatccca ggctgcctcg gatgaagaga agctcaggga
     4801 actggagcgc gcgatgagca tgccctccga aacaggcatg ccacagcaga agcgtacccg
     4861 cgcaattgtt tttgacaatc aacagcaatc cacctctcca gcggccgagc ccgctgctca
     4921 agttgctgcc tcgactgcag gctcacgaga ttgcggctcg ctacctcctg atgtgcgcac
     4981 ggtgggagtg gattttgcca tccagtttaa tgcgggtagt gcaatggttt ctgctgcttc
     5041 tgaaggcacc ttgaaccaga tcgccaaagt cctggcgctg tcaccggatc gctgtgtcat
     5101 cgtcgaagga cacaccgacg tggctggtaa ttttgacaag aacatggcgc tgtctcgcga
     5161 ccgcgctaat gcggtggtga atttcatctc cacccgaaac ggaatcgatc ggaagcgctt
     5221 tgtgccagtc ggcaagggct cgaccgaaac gatgccgaac ctcgacgcgc gcgatccgaa
     5281 aaatcgtcgc gtcgtattca aggtagtaac tggttgattc tttcgtcgta cttttatact
     5341 gaacaaggag actcacatgc gtctgatttc tatgatgatt gctgctgcca tcgttgcctc
     5401 cccggctttc gctcaacaaa cccagcgttc cagcaacccc cagggcgtgc agatccaggg
     5461 caataccgac atcaaggcta aaaacgaaaa cgtgacggcg gtcgctgtcg gcgaaggcaa
     5521 tgaagccaag aatactaccg gtgccatcaa gggtggaacc caaattcagg gtaacaccaa
     5581 gattcaggcc tcgcagaaga atgcgacggc cgttgcagtg ggcaagaaca acaccgctgc
     5641 caacgaagca ggcgtcatcg gcggtaagta agaatactcg cctgcccata gttcatcatt
     5701 tccataatgg aggaaacaaa tatgcgtact caattcgcta tcatcgctct ggcttcggct
     5761 gtcatctcta ccgctgcttt cggtcaagct tcccagactg gcccaaaggg cagcatgtcc
     5821 tcgccgaatg ctcaaggtgt tcagattcag ggcaataccg acatcaaggc taaaaacgag
     5881 aacgtaacag cggttgctgt tggtgaaggc aacaccgcga agaacacgac cggtgccatc
     5941 aagggtggga cccagattca gggcaacacc aaaatccagg catcgcaaaa gaacgcaacg
     6001 gccgttgcag tgggcaaaaa caacaccgct gccaacgagg ctggcgttat cggcggcaag
     6061 taatccactg gcacatggcc tttatggcct gccagttacc aatgatggct ggatgggata
     6121 tccccgacca gccatttttt atcggcagaa gctgcctcga tttggtttta ctgccttgct
     6181 ctaatgtcag gcttgggcgc aaaggtcatt ttcccctggg ctgtccgttg gtcgttgctg
     6241 aagattcctt cccagaaatc gccatcgggg aaaatcagtt ttcccggtcc ttctttgacg
     6301 ccttttttcc aggaaccttc gtagcggtaa ccatcctttg tcgtgtaact gccttgaccg
     6361 tggaattcgc cgtccagtat ttcgccttcg tacacatccc cgttgtcaaa gattgttttt
     6421 cccttgccgt tctgtttgtt ggactcccaa ttgccttcgt aacgaaaact ccccggtgcg
     6481 atgaagacac catggccttg cttgacaccg ttcacgagtt gcccttcaaa gcggtgtccg
     6541 ttctgccact cgattacccc cttgccactg ggtagcccac tgggttgggc aaagcgaaag
     6601 tcgccaatgt aggtagtatc gccgacctgc acacgcttgc ctaggtccgc ggttggctgc
     6661 atcgccggtg ctacaggtgc tacaggtaca acgggtgcaa cgggtgcaac ggatgaggcg
     6721 gcagccacgt aagatcgact ggagtcaggc gcaggcggaa caaggtggcc agggggaatc
     6781 attttgtcgg gcagcaatcc aggggaaata tccttggcac ggttcggttg tccaacatag
     6841 accgcattcc cagtgatacg gatcggcaca atcgtgaaac gaccggagac atctgcttca
     6901 gtacggtaga taccagctgg ggtctggaat tcgatgactt gccgaccctg cgggagcatg
     6961 gcacgagcga tcgaaatacg ttctggcagg gtacgccagt tgcggtcgtc ggcttgttcg
     7021 gtacttgcag atacgacatt ggcaaccagc ccggcgaccc agtgcgcctt gttggcttgt
     7081 tcctgaacga ccgatttgag tcccgcccgg ataaccgtgc gcaagatgat tcccggcatt
     7141 tgatccttga gcagacgccg tgccatggtg tcgatattgg ttagcgtttc gaccggatat
     7201 cgacccccgg caactgctag cgaggaaggt actggcactt gttctgaaac cttgagtagc
     7261 gggaaagaga gtggggtaat gactattcct cccttgcgca tgatggggat cgaaatattc
     7321 aacgagtcga tgcgcggcgc aaatccggtc tccaagacga atagcacgtc ggtctcgcct
     7381 aggccaggca cgcgtttgcc aatctcattg agggcagtct taatcatggt attgccaggt
     7441 gccagttgca gcgcattacg gtaacctggc tcggccagag agggttcatc ggtgacctca
     7501 aagaaataac cggccagata gtgtgcaaag gcattctgaa aaccgttctt cagctcgcga
     7561 acttccggag tgtcgagttc ctgcatcgga taaccctcaa gattgctggc ctgaccggca
     7621 tcctgtttgt tcgactcaac cttgagcgcg tcgtactcct tttctcggaa agcctcgatc
     7681 agtttttcac gttcataggt cttcttcatt tcgatgcggg catggtcgaa attaccgagc
     7741 atgatgtgat tcatggtcag gcgcgtcgag agcagcacct tttcgtagtc ctgcccatca
     7801 taacgacggg tcttgtcgct aattagaaaa ctgccaatgt caccaaatat ctttgatgga
     7861 ttgctacgaa attcgttttc ccacgcctga atgatctcgt cgcttttcat ccaggtttcg
     7921 cgactgctga tgaagttgct gccgagactc agtagttccc ccttttcgaa aaagtatagg
     7981 atgtctttgt cattgccctc gttgaagttt tcgatctttg ccacagcctg tttgagttcg
     8041 cctgttttca ccagtgtgac ggtttcgtta agcgcatcgg agtattggcg aaacatgccc
     8101 gcgacgccga tggaaggtag tccaagcatt agtaacagca atgcacaggt ataggaaatc
     8161 tgcttcatgg ttgtctcccc gatggtgttt gtaattatgt ctctggggta atgggactgt
     8221 cagatcccgg tgactaccct atagaggcgc tccttaagta agttagaccc caccgcagta
     8281 taagccatgg cctgctgcga tccgaaagag gtggccccgc ttgtttggac aggaaatgct
     8341 cctggataag tggagccctg c
//
LOCUS       DAOQJF010000126         8298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4657_length_8298_cov_4.913866, whole genome shotgun sequence.
ACCESSION   DAOQJF010000126 DAOQJF010000000
VERSION     DAOQJF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4657_length_8298_cov_4.913866"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PLF85_08085"
     CDS             <1..999
                     /locus_tag="PLF85_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNE19663.1"
                     /translation="PAAMGASFLAGSTEDNESQVKIFHDGATVDSLQGHQKALTSVID
                     SAFGAVWPKTLSEDYIKGQAPKKILVPTGLATWDGNPWTPQVLPVQVVRIGQLALAAQ
                     PTEITTMAGRRVRETVVQALSQAGVTHAVVASHSNAYSSYVTTPEEYTAQEYEGASTH
                     FGPHTLDGFLQEFKRLGEAIATGSAVAQGLYPKDYKLTQLTLQPGVVYDAAPRGKKFG
                     DVLVDAEANYRKGAKISVEFVGAHPRNDLLTMSDFAVVEKKSAGGFSHVLGDRDPQLT
                     FHWQRTRGAESKVIITWDTKEAEAGEYCIRYRGVAKSFWFGRKTKFEGVSGVFTLS"
     gene            1059..1550
                     /locus_tag="PLF85_08090"
     CDS             1059..1550
                     /locus_tag="PLF85_08090"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HNE19664.1"
                     /translation="MITRLLVIPALVFATLSANNDEQIRCDGKLLGAGRSRLEVLRYC
                     GEPQDKVAFLDERVTHRRFSNVLVGSFRETRQTGTYVDTSRSAVPPDAPGRDKEVPVL
                     AGQSSHASPSVPAHEHDDRRIVNDTTVVSQSSYTVVSSYWECQKNQCLSTNIPITLVA
                     GRL"
     gene            complement(1652..3586)
                     /locus_tag="PLF85_08095"
     CDS             complement(1652..3586)
                     /locus_tag="PLF85_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNE19665.1"
                     /translation="MKLDAKIPQGPLETKWRDYKAKLKLVNPANKRKLDVIVVGTGLA
                     GSAAAASLGELGYNVHCFSFHDSPRRAHSIAAQGGINAAKNYHNDGDSVYRLFYDTVK
                     GGDYRAREGNVYRLAELSVNIIDQAVAQGVPFAREYGGMLDNRSFGGAQVSRTFYAKG
                     QTGQQLLLGAYSALNRQIHEGTVKMYNRHEMLDLVVVNGHAKGIITRNLVTGEIKRHA
                     AHAVILATGGYGNVYFLSTNAMLSNVTASWRAHKRGAYFANPCYTQIHPTCIPQSGDY
                     QSKLTLMSESLRNDGRVWVPKDKAVAEKLQRGEITANDIAEGDRDYFLERRYPSFGNL
                     VPRDVASRAAKERCDHGFGVNATGLSVFLDFKSSIERLGEKTIAERYGNLFEMYEKIS
                     GENPYKMPMKIYPAVHYTMGGLWVDYNLMSTIPGLYVGGEANFSDHGANRLGASALMQ
                     GLADGYFVLPATIGDYLAGVGFDRPSTAGKEFDEAEENVKSQTSSMLATKGKKTVDEL
                     HRRLGHVMWEYVGMARTEAGLKKAIGEIQQIKEDFKKDVNVPGSAGSLNDSLEKAGRG
                     KDFIELGELMARDALNRNESCGGHFREEYQTEDGEAKRDDANYAYVAAWEYKGDNTPQ
                     ELHKEELKFDVVHPSQRSYK"
     gene            complement(3599..4288)
                     /locus_tag="PLF85_08100"
     CDS             complement(3599..4288)
                     /locus_tag="PLF85_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNE19666.1"
                     /translation="MAQSATGNPYLLATSLGKKYLMALSGLFLISFLPVHLAGNLLLL
                     KQDGGAAFDVYSQFMSTSPLIRVLEVTLVAGFLVHIIDGILLTLKNRAARPVGYKGGS
                     GGKPLSVFSKYMSLTGTIILIYLIIHINSFTLKHRVYEPGNVAFYHTVKSAFETGWGG
                     LYPWFYVLAMFLMALHLNHGFQSAFQSLGLNHKKYTPLIKKVGLLYSIVIPLGFAAIP
                     VYFQLRYLNIL"
     gene            complement(4385..4627)
                     /locus_tag="PLF85_08105"
     CDS             complement(4385..4627)
                     /locus_tag="PLF85_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HNE19667.1"
                     /translation="MSEKHHDINHEFPEHRDLIIKLKTSNHHFQHLADEYSKVTGEIE
                     GLEHRGSPESDAFMEGLKKKRLQLKDQLFEILQQNK"
     gene            4787..5848
                     /locus_tag="PLF85_08110"
     CDS             4787..5848
                     /locus_tag="PLF85_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE19668.1"
                     /translation="MLTRSAEQNLRYPLLLVHGLFSSSRTWRKTVRTFVDDYDLRYGG
                     EISMRPVKNPLPVTPGDFYTWNFSSNYNLSYRQQAEELARGIRQIRELNSCEKVVIAG
                     HSMGGLAARALVQLYGAQEVFALITLGTPHYGSPLALLREATQKDAQRLLGRISRLMG
                     RADNRSENNPGMLRRALMRMFRVTTEAEAEVDRFFSSEAFVELAPGSAALEELNRAEL
                     PESLRYVFITGSLTDFAAFPDAQFIARYRKLNSFWKRMATKAVNSVSNRYLLAPYEEF
                     RTYLSRYQSAAAGFPIEQLMDFDGAVPVMSQMIHHFRNPPLLKVALPVFASHNRLTKR
                     PHKLFHALAVCGAVGASAS"
     gene            5885..7153
                     /locus_tag="PLF85_08115"
     CDS             5885..7153
                     /locus_tag="PLF85_08115"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19669.1"
                     /translation="MVGDRLQLTIHELHPSGVGVGQHMGYGRIYVPFTFPGEQPEVEL
                     TDQPRTGQWNARRIASPRGDCRLAGECGSCLWPATPYAEQLRWKELLFRRAVAALPEV
                     AATKMEIHGNGGNLEFRSRVHLHANFYHGRLNFGFYARGERRLVAIDDCPVAAAPIRR
                     VIAELSSCKEGNWPQDNFGFGIELTHLAEEGCVLLVLYGAPQRRAALESALPEFTALP
                     SRPRALIAFAEDNPAFIWQRLAGATMHTRPGCFQQVNRSQSNRVRSLITERLGQVGGG
                     VFFDLYSGSGNYSLPLHSLAGAIFGCDDNPVGIALAHYNVQQNAVFHAHYLCADAGQV
                     MTAPEQYGWPNRADFIVCDPARFGMAKELARLIAQARPKELVLVSNNTAAFVKDARGL
                     ISAGFAPVKIHLVDFFPNTPHWNAVSVWKM"
     gene            7241..7314
                     /locus_tag="PLF85_08120"
     tRNA            7241..7314
                     /locus_tag="PLF85_08120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7275..7277,aa:Arg,seq:acg)
     gene            complement(7434..>8298)
                     /locus_tag="PLF85_08125"
     CDS             complement(7434..>8298)
                     /locus_tag="PLF85_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE19670.1"
                     /translation="QPFIMNAISKLVADWFEPEHNALATGLGTLGMFIGMALGLGLAV
                     PFTQAFGFSGSMLAYAIMSVASTVLFVLFARENRGLRDSATAEMARDLKLLMKSRNLV
                     RLFAIAFLALGYFNGFTSWLEQILAPNGVSAESAGNAGASLIVGGILGAAIIPAISDA
                     AKRRKPFLVACCIMAGALTWPLSRSGDGIQLYAMSSLLGFFFLPGYALLLSATEEFAG
                     KDKAGSATGLLMLFGNFGGVVVSILMDAVKAGPRDWRNAVYLLLGTIGLSLLLALITG
                     ESFHGRSSQYS"
BASE COUNT         1773 a         2253 c         2386 g         1886 t
ORIGIN      
        1 cccgccgcga tgggcgcgag ctttcttgcg ggcagtaccg aagacaacga atcgcaggta
       61 aaaatattcc atgacggagc gaccgtcgat tcgctgcagg gccaccagaa ggctctgacc
      121 tccgtcattg attcggcttt tggcgccgtc tggccaaaaa ctctctctga agactatatt
      181 aaggggcagg cgcccaaaaa aattctcgtg cccaccggcc ttgccacctg ggatggtaat
      241 ccctggacgc cgcaggtact gcctgtacag gttgtgcgta tcgggcaact tgcgctggca
      301 gcgcaaccga cagaaatcac cactatggcc ggccgccgcg ttcgcgaaac cgtggtgcag
      361 gccctttcgc aggcaggggt cacgcacgcc gtggtcgcgt cgcattccaa cgcgtattct
      421 tcgtacgtga ccacacctga agaatacacc gcccaggaat acgaaggtgc ttcgacccat
      481 tttggcccgc acacgctcga cggtttttta caggagttca agcgcctcgg ggaggcaatc
      541 gccactggtt cagcggtcgc gcagggtttg tacccgaaag actataaact cactcagctg
      601 acgctgcagc cgggggtcgt gtacgatgcc gctcctcggg gtaagaagtt tggcgacgtg
      661 ttagtcgatg ccgaggccaa ctatcgcaaa ggtgcaaaaa tatccgtaga atttgtcggg
      721 gctcacccgc gcaatgatct cttgaccatg tctgactttg ccgtagtcga gaagaagtca
      781 gctggcggat tctcacatgt tcttggcgac cgtgacccgc aactgacctt tcactggcaa
      841 cgcacacgcg gcgctgaatc gaaggtgata ataacctggg atactaaaga agcggaagca
      901 ggcgaatact gcatccgcta ccgcggtgtg gcgaaatcat tctggtttgg cagaaagacg
      961 aaattcgagg gcgtgagcgg cgtttttacc ctcagctgaa ggatagccga taaaaaatgg
     1021 attgcccggg cgttatccgg ggtctgcacc gtacctctat gataacgcgc cttctggtta
     1081 tacctgcgct ggtttttgcg acactcagcg caaataatga cgagcagatt cgctgtgacg
     1141 gcaaactgct cggtgcgggc cgatcgcggc tcgaggtttt gcgctattgc ggtgagccgc
     1201 aggacaaagt ggcctttctc gatgagcgcg ttactcaccg caggttttca aacgtgctgg
     1261 taggaagttt cagggaaacg cggcagacag gaacctacgt cgatacctcg cgcagcgctg
     1321 tgccccctga tgcgccgggg cgggacaaag aggtgccggt tcttgcgggg cagtcgtcgc
     1381 atgccagccc gtcggtacca gcgcacgagc atgacgacag aagaatcgtc aatgacacga
     1441 cagtcgtgag ccagagttct tataccgtag tatcttcgta ctgggagtgc caaaaaaatc
     1501 agtgcttgtc gacgaatata cctataactt tggtagcggg aagattatga cattcgtgcg
     1561 cttcgaaaac ggtcgcatca ggagcatcaa gttcggcgaa tacggttttt gaatcaattt
     1621 gggcgaggta tctgcctcgc ccaggcacga cttatttgta acttcgctgg cttgggtgaa
     1681 caacgtcgaa tttgagctct tctttatgca gctcttgcgg cgtgttatca cccttgtatt
     1741 cccaggctgc cacgtatgcg tagttggcgt catcgcgttt cgcttcgcca tcttcagtct
     1801 ggtactcttc acggaagtga ccaccgcatg actcgttacg gttgagcgca tcgcgtgcca
     1861 tcagttcgcc gagttcgatg aagtctttcc cgcggcccgc tttttcaagc gagtcattga
     1921 gcgagcctgc gctgcccgga acattaacgt ctttcttgaa atcttctttg atctgctgta
     1981 tttcgccgat ggcctttttg aggcctgctt cggtacgtgc cataccgacg tattcccaca
     2041 tgacgtggcc gaggcggcgg tgcagttcat cgacagtttt cttacccttg gtcgccagca
     2101 ttgaagatgt ctgcgatttt acgttctctt ctgcctcgtc gaattcttta ccggcagtcg
     2161 atgggcggtc aaagccaaca cctgcgaggt agtcaccaat tgtagcagga agtacaaagt
     2221 agccgtcggc aagaccttgc atgagcgccg aagctcccag gcggtttgca ccgtggtctg
     2281 agaagttggc ttcgccaccg acgtacagac cgggaatggt cgacatcagg ttgtagtcta
     2341 cccagaggcc acccatggtg tagtgcacag cggggtaaat tttcatcggc attttgtatg
     2401 ggttttcgcc cgagattttt tcgtacatct cgaacaggtt accgtaacgt tccgcgatgg
     2461 tcttttcacc gaggcgctca atagatgact tgaagtcgag aaagaccgaa agaccggttg
     2521 cattcacgcc aaaaccatgg tcgcagcgtt ctttagctgc gcgcgaagcc acgtcgcgtg
     2581 gaaccaggtt gccgaatgaa ggatagcggc gttcaagaaa gtaatcgcgg tcaccttctg
     2641 caatgtcgtt tgcagtgatc tcgccgcgct gcagcttttc cgctacagcc ttgtcttttg
     2701 gtacccaaac gcggccgtcg ttacgcagcg actcactcat cagggttaat tttgactggt
     2761 aatcgccgct ctgaggaatg caggtcgggt ggatctgcgt gtagcatggg ttggcaaagt
     2821 aagcaccccg cttgtgtgcg cgccacgagg ccgtcacgtt cgagagcata gcgttcgttg
     2881 ataggaagta gacgttaccg taaccaccgg ttgcaagaat cactgcgtgc gcggcgtggc
     2941 gcttgatttc gccggtgacc aggttacgcg tgatgatgcc tttcgcatga ccgtttacta
     3001 ccacgaggtc gagcatctca tggcggttat acatctttac agtgccttcg tggatctgcc
     3061 ggttcagcgc gctgtatgca ccgagcagca gctgctggcc agtctggcct ttcgcgtaaa
     3121 aggtccttga aacctgagct cccccgaatg aacggttatc gagcatgccg ccgtattcac
     3181 gcgcaaaggg aacgccctgc gccactgcct ggtcgatgat gtttaccgaa agttctgcga
     3241 ggcggtagac gttgccttca cgcgcgcggt agtcaccgcc cttaaccgtg tcatagaaca
     3301 ggcggtagac ggagtcaccg tcgttatggt agttctttgc agcgttgata ccaccctgcg
     3361 cagcaatcga gtgggcgcgg cgcgggctgt cgtggaaaga gaagcaatgt acattgtagc
     3421 cgagttcgcc gagcgaagcg gcggctgccg aaccagcgag ccccgtaccc acgacgatga
     3481 cgtcgagttt acgtttgttg gccgggttta ccagcttcag ttttgctttg taatcgcgcc
     3541 acttggtttc gagtggccct tgtggaattt ttgcgtccaa tttcatgtta tggtttcctt
     3601 atagaatatt cagatatcgc agctggaaat acacggggat tgcggcgaat ccaagcggga
     3661 tgacaatcga gtagagcagc cccaccttct tgatgagcgg agtgtatttt ttatggttca
     3721 ggcccagcga ctggaatgca ctctggaagc cgtgattcag gtgcaacgcc atcaggaaca
     3781 ttgccagaac atagaaccag ggatagaggc cgccccagcc ggtttcaaag gcgctcttga
     3841 ccgtatggta gaaagccaca ttgcctggct cgtaaacgcg gtgtttcagc gtgaatgagt
     3901 tgatatgaat gataaggtag ataaggatga tggtgcctgt caggctcatg tacttggaaa
     3961 atactgacag gggtttgcca cctgaaccgc ctttatagcc aacgggccgg gctgcccggt
     4021 tcttcagagt gagcagaata ccgtcaataa tgtggaccag gaaaccggcc acgagagtca
     4081 cctcgagtac cctgatcagg ggtgaagtcg acatgaactg cgagtagacg tcgaaggctg
     4141 ctccgccatc ctgcttgagc agcagcaggt tgccggccag gtggacaggt aaaaagctga
     4201 tgagaaacag gcccgaaaga gccattaggt atttttttcc gagtgaagtt gcgagcaggt
     4261 acggattgcc ggttgcggac tgagccatgc ggtaaacacg aacaaattgc atagtgcgaa
     4321 aacaaaaaaa gcctccgggg gtgcccggcg gcttttcgtg taatgccctg gggcaaaatt
     4381 tcagttactt gttctgctgc agaatttcaa aaagctgatc tttcagttgc agacgcttct
     4441 ttttgagccc ttccataaag gcatccgatt cagggcttcc cctgtgttcc aggccctcaa
     4501 tttcacccgt aaccttgctg tactcatctg caaggtgctg gaagtgatgg ttggaggtct
     4561 ttagtttgat aatgaggtcg cggtgttccg ggaactcgtg gttgatgtcg tggtgttttt
     4621 cagacataat tcctcttgaa tgtagtgtaa gctgtttgtc gctatggagc atgcagacat
     4681 ggcgtaagtc ttttttccct gcgcctgctg gtcgccggtg caggggttca gcgaggccaa
     4741 tttggaaaac gctttttgcc ctgccgcgcc tgcgctgtct gcctgcatgt taacccgctc
     4801 tgcggagcag aacctgcgct atcccctgtt gcttgtgcac ggcctgtttt cgagcagccg
     4861 tacctggcgt aagaccgtaa ggactttcgt cgacgattac gacctgcgtt acggtggcga
     4921 aatcagtatg cggccggtga aaaacccgct gccggttacc ccgggtgatt tttacacgtg
     4981 gaatttttcg agcaactata atctgtcgta ccgccagcag gccgaagaac tggccagggg
     5041 catccggcag atacgcgaac tcaatagctg cgaaaaggtt gtcattgcgg ggcacagtat
     5101 gggtggtctt gccgcccgtg cgctggtaca gctttatggt gcgcaagagg tgtttgccct
     5161 cattactctc ggtactccac actatggcag cccgcttgcc ctgctgcgcg aagcgacgca
     5221 aaaagatgcg cagcgcctgc tcggtcgcat tagccggctc atgggcaggg ccgacaaccg
     5281 cagcgagaat aatcccggta tgctgcggcg cgcgttgatg cggatgttcc gcgtgacgac
     5341 agaagccgag gccgaggtcg atcgcttttt ttccagtgaa gcttttgttg aacttgcgcc
     5401 cggcagcgca gcgctcgaag aactgaacag ggcagagcta cccgagtcgc tgcgttatgt
     5461 ctttatcacc ggtagcctga ccgatttcgc tgcttttccc gatgcgcagt ttattgcgcg
     5521 ctaccgtaag ctcaacagtt tctggaaacg catggcgacg aaggcggtaa acagcgtgag
     5581 caatcgctac ctgttggccc cgtacgaaga attccgcaca tatctgagtc gctaccagag
     5641 cgccgcggct ggttttccga ttgagcagct gatggatttc gacggggcgg tgccggtcat
     5701 gagccagatg atccatcact tccgcaatcc gccgctactc aaagtagcgc tgccggtctt
     5761 tgccagtcat aaccggttga cgaagcgccc gcataaactt tttcacgcgc ttgcggtatg
     5821 cggtgcggtg ggagcttcgg cgtcctgaaa atcgacacga ccaccaaggc ttgtaaccag
     5881 aaacatggtc ggtgaccgcc tgcagctaac gattcatgaa ctgcacccat cgggtgtggg
     5941 cgttgggcag cacatgggct acggccgtat ctacgtgccg ttcacctttc cgggtgagca
     6001 gcccgaggtt gaactcactg accagccgcg cacaggacaa tggaatgcgc ggcgtatcgc
     6061 gagtcccagg ggcgattgtc gcctcgcagg ggagtgtggc agttgcctgt ggcccgccac
     6121 cccttacgcg gaacagctgc gctggaaaga gctgctcttc aggcgcgctg ttgctgcgtt
     6181 accagaagtc gccgcaacaa aaatggaaat ccatggcaat ggcggcaacc tcgaattccg
     6241 tagccgtgtg cacctgcatg caaacttcta ccatggcagg ttgaactttg gtttttatgc
     6301 gcgtggtgag cgacgcctgg tggcgatcga tgattgcccg gttgccgcag cgccgatacg
     6361 ccgcgtcatc gcggagcttt catcctgtaa agagggtaac tggcctcagg ataattttgg
     6421 ttttggtatt gagctgacac accttgcgga agagggctgt gtactgctcg tgctgtacgg
     6481 tgcgccgcag cggcgggcgg cgcttgaatc agccctgcca gaatttaccg cgttgccatc
     6541 tcgtccgcgg gcgctgatcg ccttcgccga agacaatcct gcttttatct ggcagcgcct
     6601 ggcaggtgca acgatgcata cgcgcccggg ttgttttcaa caggtgaacc ggtcgcagag
     6661 caatcgtgtg cgcagcctga taaccgaacg tctcgggcaa gttgggggtg gagttttttt
     6721 tgacctctac agcggcagcg gtaactactc gctgccgctg cattcgcttg cgggggcaat
     6781 ttttggctgc gacgataacc ccgtcggcat tgcgctggcg cactacaacg tgcagcagaa
     6841 cgctgtattc cacgcgcatt acctctgcgc cgacgcgggc caggtcatga ctgcgcccga
     6901 acagtacggc tggcccaacc gggcggattt catcgtctgc gaccctgcgc gctttggcat
     6961 ggcgaaggaa ttagcgcgct tgatcgcgca ggccaggccg aaagagctgg tcctggtttc
     7021 gaacaatact gccgcatttg ttaaagacgc ccgaggcctc atcagcgccg ggtttgcccc
     7081 tgtgaaaatc cacctggtgg attttttccc caatacaccg cactggaatg ccgtaagtgt
     7141 gtggaaaatg tgaagtcggt tgacgccctg tttcagggcc gtttttggcc ctcacccaag
     7201 cgcccgtagc actgtcaaca gagctacgat cgctgaagca gcgcccgtag ctcagctgga
     7261 tagagtgtca gactacgaat ctgaaggtca atggttcgac tccattcggg cgcgcacttg
     7321 atgcacctcg tgtgcatacg cccccacgga cttcgtgtcc ttcgggcgcg cattggatgc
     7381 acttcctgtg catgcgccct cacggacatc ctgccctctg cctcttctga tacctaggag
     7441 tactggctac ttctgccgtg gaaggattcg ccggtaatca gcgccagcag caaagataat
     7501 ccaatcgtgc cgagcagcag gtacaccgca ttacgccagt cgcgtgggcc cgctttaaca
     7561 gcatccatca gtatactgac aacgaccccg ccaaaattgc cgaagagcat aagaagcccg
     7621 gtcgctgatc ctgccttgtc tttgcccgca aattcttcag ttgcagagag caacagcgca
     7681 taaccgggta aaaagaaaaa gccaagcaag gagctcatcg cgtagagctg gataccgtcg
     7741 cccgagcggc tgagcggcca cgtcaatgca cccgccatga tgcagcaggc aaccaggaac
     7801 ggcttgcggc gctttgctgc atctgagatt gccggaataa tcgccgcacc cagaataccg
     7861 ccgacgatta gcgatgcacc agcattgccg gcagattctg cgctgacacc gttgggcgcc
     7921 agtatctgct cgagccagct cgtgaaaccg ttgaaatacc ccagcgccag gaaggcgatg
     7981 gcaaagaggc gcaccaggtt gcgggatttc atgaggagtt tcagatcgcg tgccatctct
     8041 gcggtagcac tgtcgcgcaa gccgcggttt tcgcgcgcaa acaagacaaa gagaaccgtg
     8101 ctggcgacgg acatgattgc gtacgcgagc atggagcctg agaagccgaa tgcctgcgtg
     8161 aacggtaccg cgaggccaag gccaagcgcc ataccgataa acatgccgag cgtacccaga
     8221 cccgtagcca gcgcgttatg ttcaggttca aaccagtcag caacgagttt ggaaattgca
     8281 ttcatgatga aaggctga
//
LOCUS       DAOQJF010000127         8111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4793_length_8111_cov_5.606132, whole genome shotgun sequence.
ACCESSION   DAOQJF010000127 DAOQJF010000000
VERSION     DAOQJF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_8111_cov_5.606132"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /gene="rpsI"
                     /locus_tag="PLF85_08130"
     CDS             <1..285
                     /gene="rpsI"
                     /locus_tag="PLF85_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNE19671.1"
                     /translation="EYFTRPELRAAVMQPLLLTERAAQTDVHVNVYGGGVTGQAQAIA
                     LGIARSIDQSDSSQHSKLKAAGLLTRDPRMVERKKYGFHKARRATQFSKR"
     gene            complement(341..1609)
                     /locus_tag="PLF85_08135"
     CDS             complement(341..1609)
                     /locus_tag="PLF85_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19672.1"
                     /translation="MSPGRFLGALLVLLLTQACSKYVYEHKEGRLSRGTFNFMKAYYY
                     QGQFEEEETVEARNRQILNDLADLDGIGHLGTTKGLKEDPYYDEYMYRQQHARHGKAA
                     VGYWQQKYKDKFNWQMLFSAENLHYDEKRFLREFRTTHLSGGKISDVLVATFEGRPLH
                     YHDLKRVMTVSDYAQFPNYTEQALQSGMRDVLKAWLQKQIHDRMVWQMSADERELLRF
                     DHNRVAVLFLKVKYGKAGKGIYPGGMEKIPLTPNEIYDHFHKMQNTLADVLWVKAAYT
                     VVAEENLAEELIAKLDKGGNFPELAARYAMAPKFIPTAKPTVYQGYDRSKDIEAREKR
                     DYYDRLIIDMAGRDVTKPEPYLGRDGIVIVRIYDVSRALEKIRLQDVSWKVENDLRLK
                     LLGEVYEHDVRDARDWLKIQYNDWAIKRLP"
     gene            complement(1609..1941)
                     /locus_tag="PLF85_08140"
     CDS             complement(1609..1941)
                     /locus_tag="PLF85_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19673.1"
                     /translation="MTVRLRMCFVLLALTATLHAKAGAREDDKPIAGVEAAIRHSLES
                     QYFSGGQVQELLLAEPESHGSNSTYLGEFAIRRAGRLIRCEDWHFTVHRVPEGWALLK
                     TERGRCND"
     gene            2058..2639
                     /locus_tag="PLF85_08145"
     CDS             2058..2639
                     /locus_tag="PLF85_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19674.1"
                     /translation="MEFVASILVLAFTAALILLLNYYRRLLGDGRREYPREELIYAHN
                     FIINEWQRIVSADPATGYAGAIAGALLGYMLSYTGGVYGEHYESAFFHSAILPGLWFI
                     GQPYLRDKAEEMQWPPFVRKFIDNDITFFFGLSAIIGSQSLVAYGFYHALSFLWVFFN
                     YVVLLALVLYYFHKRDNNEKGGLPDRQRRENRI"
     gene            2639..3097
                     /locus_tag="PLF85_08150"
     CDS             2639..3097
                     /locus_tag="PLF85_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE19675.1"
                     /translation="MLSTTHDQQFILFRVEGETYAVGVNETHEVIRYKQPKRIPHAPA
                     HVLGVINLRGAIIPVVGLRERFSLESKPADDETRVIVAAQNGRLTGLVCDAVERVVAI
                     PAANIEENPDIEQRRGLSAIRGVAHLDEEDSVVFLLALDMVLAQTGENVP"
     gene            3094..4296
                     /gene="ribD"
                     /locus_tag="PLF85_08155"
     CDS             3094..4296
                     /gene="ribD"
                     /locus_tag="PLF85_08155"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNE19676.1"
                     /translation="MTEASDRAALSAAYAQAISAAGNSDPNPSVGAVIVDTHGKTVAG
                     GYTQRAGYAHAERHALAQLPQPDLHGHTMYVTLEPCCHHGRTPPCVDAIVERRIGRVV
                     IAERDFAAEVQGRSLTLFREQGIDAVEWPAEDFQREKWFTTGPFFFARKHRRPRVVLK
                     WAQTSDGSIAPQNGKSGPISGSDAALLTATLRFFFKLTVASPGTVLVDSPRLTVRTAG
                     DLPELSQSGLTAFAWELLRRQYQLACEQPDAETLKRRIRSPESLFLLPDTMDGFMAGM
                     PGNHQATRQNWRSKPIPRAEWQQDFAGTWNSVLAEVLAGGFNSLLLEAGPAFSEAMLQ
                     HDLADALVIYRSRVKSDLQLWGGQGRANHVSRILAATESPTLPGYELLEFARLEQDDF
                     FLFVRAGR"
     gene            4299..5147
                     /locus_tag="PLF85_08160"
     CDS             4299..5147
                     /locus_tag="PLF85_08160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE19677.1"
                     /translation="MTRVDVVIPAYGRQHLLGEAIASVQQQSLSDWHLWVIDDASAEA
                     VAIPSGADSRVEIRRLPKNGGPAYARNFGAALGKAPFIAFLDSDDLWHPEKLQHALAA
                     FAHDPELLWWHSNELWLRNGQPAKQKAIHRKQGGQFFERALERCLISPSAVVFRRDFF
                     MAQGGFAPAFRLCEDYELWLRLLLYSPVGYSTEPLTIKRAGDWPQLSSAREIDRYRVL
                     AMHRVWRLKRSVMPEAWQSALISECKKKCELLLAGAQKHQNERGQRRYQAWLTLFNTL
                     RTRPIR"
     gene            complement(5096..6385)
                     /locus_tag="PLF85_08165"
     CDS             complement(5096..6385)
                     /locus_tag="PLF85_08165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19678.1"
                     /translation="MRLWLILWCLVLTLPFDPARAEVYPLTLYRSYGDMQSQPGLLVG
                     KINTKGSDNSIPPRQILGYDVRMSVVTASVDNQAKIYPGMKVYIVKRERDHQKNRAAL
                     IVAEGEIDSISDTVFSGRVAQIRGQFSMVSRQHFVAVPQVAKSREGDQKSAQEYFLES
                     ERHRHEKDFARSAQKLQHARELEPNNPLVNLRYAELALQNGAEDKARAALRKAFDERR
                     RLEDVNEYLKLGALYLAAEVRALPLKSQDILKAGMALLAATRQFEKDLGQFGKDLTPP
                     KSSTFRLRHSRFSAQYHLHYGILLRQIAGALREYSPEAITRVLGYGERDVLYAPIETR
                     VARERVLALPRKAWDRAYSDAAITHFELALRKDRQSEAAYELIDLCDSLWDGSDNNRR
                     QQLKEIITRYAPAYLETAHEDQRMGRVRSVLNKVSQA"
     gene            6753..7898
                     /locus_tag="PLF85_08170"
     CDS             6753..7898
                     /locus_tag="PLF85_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE19679.1"
                     /translation="MPRLLPFTEEHDRFREMVADFIAKECVPRHEEWEKAGEVSRDVW
                     EKAGQLGMICPNFPEEYGGMGLDFLYNVIVIEELAKAASSGFFISLHADVIAPYVLHY
                     ANEEQKKRWLPGIINGTKILSIGMTEPGTGSDLAAVTTKAVDMGDHYLLNGSKTFISN
                     GYLSDLCIVVARTGEGQGGISLLMVERGMPGFERGRKLKKIGLHAQDTSELHFNDVKV
                     PKANLIGKLNAGFRYLMMSLAQERLVLAISNIASAETVLDQTVKYCNERKVFGKPVGS
                     FQNTRFSLTDMYVEQEAARSYCDRVVMAFMAGEKVTAEASAVKLQCSEMLQAHVTKCL
                     QFFGGYGFMMEYPIAKAYLDCRVQTIYAGTSEIMREIVSKNLGLAST"
     gene            7915..>8111
                     /locus_tag="PLF85_08175"
     CDS             7915..>8111
                     /locus_tag="PLF85_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19680.1"
                     /translation="MPAAKGGRRHLISLNRWGILSRYFLSGLFLLSLFHCTSEHNTVR
                     LYPAKSDKSSYRQLSTEHYLYV"
BASE COUNT         1747 a         2309 c         2265 g         1790 t
ORIGIN      
        1 gaatacttca cacgccctga actgcgcgcc gccgtgatgc aaccgctgct gctcaccgag
       61 cgtgcagccc agactgatgt ccacgtgaat gtttacggag gcggagttac tggccaggct
      121 caggcgattg cgctgggcat cgcacgcagc atcgaccagt cagacagctc acagcattca
      181 aagctcaagg cagccggcct tcttacccgt gacccgcgta tggtcgagcg taagaaatac
      241 ggattccaca aagcacgccg cgctacccag ttcagcaagc gctgatacga ggcacggtca
      301 acaagagaag ccggttggtt gccaaccggc ttttttttta tcatggcagg cgtttgatcg
      361 cccagtcgtt atactgtatc ttcagccagt cccgcgcgtc gcggacatcg tgttcgtaga
      421 cttcaccgag tagcttcaac ctgaggtcgt tttccacctt ccagctgaca tcctgcagcc
      481 tgatcttttc gagcgcacgg gaaacatcat agatgcgcac gatgacaatg ccatcgcggc
      541 caaggtaggg ttcgggcttt gtgacgtcgc ggccggccat gtcgataatc aggcggtcgt
      601 aataatcacg tttttcgcgg gcttctatgt ccttcgagcg gtcataaccc tggtacaccg
      661 tcggttttgc cgtggggatg aattttggcg ccattgcgta gcgcgcggcg agttcgggga
      721 aattaccgcc tttgtcgagt ttggcgatta actcttccgc caggttttct tctgcaacaa
      781 cggtataggc tgccttaacc caaagtacat ccgccagtgt gttctgcatc ttgtggaagt
      841 gatcgtaaat ttcgtttggt gtgaggggta ttttctccat accacccggg tagatgcctt
      901 tgcctgcttt gccatatttg accttgagaa aaagtactgc cacgcggtta tggtcaaagc
      961 gtaaaagttc acgctcgtct gcgctcattt gccagaccat gcggtcgtgt atctgctttt
     1021 gcagccacgc cttcagtaca tcgcgcatac ctgactgcag cgcctgctct gtgtaattcg
     1081 ggaactgggc atagtccgag actgtcatga cacgtttgag atcgtggtaa tggagcgggc
     1141 gaccttcaaa agttgccaca agcacatcgg agattttgcc gcctgacagg tgtgtggtgc
     1201 gaaattcgcg caggaatctc ttctcgtcgt aatgcaggtt ctcggcgcta aagagcatct
     1261 gccagttaaa cttatcttta tatttttgct gccagtagcc tactgcggct tttccgtgcc
     1321 tggcgtgctg ctgccggtac atgtattcgt cgtagtacgg atcttctttg agacctttgg
     1381 tcgtgccgag atggccgatg ccatcgaggt cggccaggtc gttgagaatc tgcctgttgc
     1441 gcgcctcgac ggtctcttct tcttcaaact ggccctggta gtaatatgcc ttcatgaaat
     1501 tgaaagtgcc acgcgagagg cgaccttctt tgtgttcgta gacatacttg gagcacgcct
     1561 gcgtcagcag caggacaaga agagcgccca aaaaacggcc cggtgacatc aatcgttgca
     1621 tctgccgcgc tccgttttta gcaatgccca cccctctgga acgcggtgca ccgtgaagtg
     1681 ccagtcttca caacggatca gccggcccgc gcgcctgatc gcaaattcac ccaggtacgt
     1741 cgaattagaa ccatgggatt caggctcagc aagcaggagc tcctgcacct ggccaccgga
     1801 aaagtactgg ctttcgagcg aatggcggat agctgcttcg acaccggcaa taggtttgtc
     1861 gtcttcgcgt gcacccgcct ttgcgtgcag cgtggcagta agcgccagca gcacgaaaca
     1921 catacgcaat cggacggtca tgggcacctg cgttcagggt gtaggttgag cggcaagtcc
     1981 tttacaccaa ggggagtgca agcagacgac gccctgaatt ttgattgtat agctgctttt
     2041 gcgccgcaga atggtttatg gaatttgtcg cctcgattct ggtgctggcg tttactgccg
     2101 cgctgatcct gctgctgaac tactaccgca ggttgctcgg cgacggccga cgcgaatacc
     2161 ctcgcgaaga gctgatctac gcgcacaact ttatcatcaa tgaatggcag cggattgtca
     2221 gcgctgatcc ggcgacgggt tacgcgggtg caattgcggg cgcactgctc ggttatatgc
     2281 tgagctacac cggcggagtt tacggcgaac actacgaaag cgcctttttc cattcggcga
     2341 ttctgcctgg tctatggttc atcggccagc cttacctgcg tgacaaggca gaagaaatgc
     2401 agtggccgcc atttgtccgc aagttcatcg acaacgatat tacgttcttc tttgggctca
     2461 gcgcgatcat cggcagccag tcgctggtgg cttacggttt ctaccacgcg ttgagcttcc
     2521 tgtgggtttt tttcaattac gttgtgctgc tcgctctcgt gctctattat tttcataaac
     2581 gcgacaacaa cgagaaaggc ggccttcccg atcgccagcg ccgggaaaac cgcatttgat
     2641 gctgtccacc acgcacgacc agcagtttat cttgttccgc gtagaaggtg aaacctacgc
     2701 ggtcggcgtc aacgagacgc atgaagtcat acgttacaag caaccaaaac gtatccccca
     2761 tgcgcccgcg cacgtgctgg gtgtcatcaa cctgcgcggt gcaattattc ctgtcgtcgg
     2821 cctgcgcgaa agattttcac tcgaatcgaa acctgcggac gacgagaccc gtgtcatagt
     2881 cgccgcgcaa aacggccggc tcacgggcct cgtatgcgac gccgtcgagc gtgttgtcgc
     2941 cataccggca gcgaatattg aagaaaatcc tgacattgaa caacgtcgcg gattaagcgc
     3001 cattcgtggc gtggcgcacc tcgacgaaga agacagcgta gtttttttac tggcactcga
     3061 catggtgctc gcgcagaccg gcgaaaacgt cccgtgaccg aggctagtga ccgggctgca
     3121 ctctctgcag cttacgcgca ggcaatcagc gcggcgggca attccgatcc gaatccttct
     3181 gtaggggcgg tcatcgtcga cacgcacggc aagacagttg ccggcggtta tacccagcgg
     3241 gcgggttacg cacacgccga acggcatgct ctcgcccagt tgccacagcc tgacctgcat
     3301 ggccatacca tgtacgtgac tcttgagccc tgctgccacc acgggcgtac accgccgtgc
     3361 gtagacgcaa ttgtggaacg caggatcggg cgcgtcgtta tcgcagagcg tgactttgcc
     3421 gcagaggtgc agggtcggtc cctgacacta ttccgggaac aaggtatcga cgctgtcgag
     3481 tggccagcag aagattttca gcgcgaaaaa tggttcacga ccggcccatt ctttttcgcg
     3541 cgcaagcaca ggcggccgcg cgttgtactg aaatgggcgc agaccagcga tggctccatc
     3601 gcaccgcaaa acggaaaatc gggaccgatc agcggcagcg acgcagcact gctcacagca
     3661 acactgcgct tcttttttaa actcactgtt gcttcgccgg gtaccgtgct ggttgactcg
     3721 ccgcgcctca ccgttcgcac cgcaggcgat ttacctgagc tttcacagtc cgggctgact
     3781 gcttttgcgt gggaacttct gcggcgacag taccagctcg cgtgtgaaca gcctgacgcc
     3841 gaaaccctaa agcggagaat acgatcaccc gagagtctgt ttcttttgcc cgatacgatg
     3901 gacggcttca tggcgggaat gcctggcaat caccaggcaa cgaggcagaa ctggcgctcg
     3961 aaaccaatcc cccgcgcaga gtggcagcaa gactttgcgg gtacctggaa ttcggtactg
     4021 gccgaagttc ttgccggtgg ttttaacagt ctcttgctcg aagccggccc ggcattcagt
     4081 gaagccatgc tgcagcatga cctggccgat gctctggtaa tctaccggtc gcgcgtgaag
     4141 agcgacctgc agttgtgggg cgggcagggc cgcgctaacc atgtgagccg catcttagca
     4201 gctacagaat ctcccacgct gccaggttat gaactcctgg agtttgcccg cctggagcag
     4261 gacgattttt tcctctttgt gcgcgcaggt cgctgaccat gactcgcgta gacgtcgtca
     4321 taccggccta tggccggcag catttgcttg gcgaagcaat tgcctcggtt cagcagcagt
     4381 cgctgagtga ctggcacctg tgggtcattg acgacgcctc ggcagaagct gtcgccatac
     4441 caagcggggc agacagccgg gtagaaatcc gacgcctgcc gaaaaacggc gggcctgcgt
     4501 acgcacgcaa ctttggagcc gcgctgggca aagcgccttt tatcgcgttt ctcgacagcg
     4561 acgatctctg gcacccggaa aagctccaac acgctttggc agcgtttgca cacgacccgg
     4621 aactcctgtg gtggcacagc aacgaactat ggctgcgcaa cggccaaccg gcgaaacaga
     4681 aagcaatcca ccgcaaacag ggtggccagt tctttgagcg ggcgcttgag cgctgcctca
     4741 tctcgccttc ggcagtggtt tttcggcggg atttctttat ggcacagggc gggtttgccc
     4801 cggcattcag gctgtgcgag gactatgaac tctggctgcg gcttttgctc tactcgccag
     4861 tgggttacag cactgaacca ctcaccatca agcgcgcggg tgattggccc caattgagca
     4921 gcgcacggga gatcgaccgt taccgggtgc tcgcgatgca tcgcgtgtgg cgtttgaaac
     4981 gctcagtcat gcccgaggca tggcaaagcg cattgatcag cgagtgcaaa aagaagtgcg
     5041 agttgctgct ggcaggtgcg cagaagcacc agaacgaacg cggccaacgc cgctatcagg
     5101 cctggctcac cttgttcaat acgctgcgca cgcggcccat acgctgatct tcgtgagcgg
     5161 tctcaaggta ggccggcgcg taacgggtaa taatttcctt tagctgctgg cgccggttgt
     5221 tgtcagaacc atcccagagt gaatcgcaca ggtcaatgag ctcataggca gcttcgcttt
     5281 gcctgtcttt cctgagcgcc agttcaaaat gagtgatggc cgcatcgctg tatgcgcgat
     5341 cccacgcttt acgcggcaaa gcgagcacgc gctcgcgggc tacgcgggtt tcgatcgggg
     5401 catacagaac atcacgttcg ccgtagccca acaccctggt gatagcctcg ggggagtact
     5461 cgcgtaacgc gcctgcaatc tgcctgagca agatgccgta gtgcaggtgg tactgcgcag
     5521 aaaagcggct gtgcctcagc ctgaacgtcg atgacttggg cggagtcagg tctttaccaa
     5581 actggcccag atccttttca aactgtcgcg ttgccgccag tagcgccatg ccggccttga
     5641 gtatatcctg gcttttgagt ggcagcgcac gcacctcggc ggccaggtag agcgcaccca
     5701 gcttcaggta ctcgttaacg tcttcgagcc ggcgcctctc atcgaaagcc ttgcgcaggg
     5761 ctgcgcgggc cttgtcttca gcgccgttct gcagcgcgag ctctgcatag cgcaggttga
     5821 ccagcgggtt gttgggttcg agctcgcgcg cgtgctgcag tttctgcgcc gagcgggcga
     5881 aatctttttc gtggcggtgg cgctccgact cgagaaagta ttcctgcgcg cttttttggt
     5941 cgccttcgcg cgacttcgcg acctgcggca ccgcgacaaa atgctgccgt gagaccatcg
     6001 aaaactgccc gcgtatctgt gccacacggc ccgagaagac ggtatcagat atcgaatcga
     6061 tttcaccttc ggcgacgatc agtgcggcgc ggttcttctg gtggtcgcgt tcgcgcttca
     6121 cgatgtagac cttcatgcct gggtagattt tcgcctggtt gtcgaccgag gcggtcacca
     6181 ccgacatccg cacatcataa cccagaatct ggcgcggggg aattgaattg tccgagccct
     6241 tggtgttaat cttaccgact agcaggccgg gttgcgactg catgtcgcca taactgcggt
     6301 agagcgtgag cggatatacc tctgcacggg cagggtcaaa aggcaaagtc agtactaaac
     6361 accaaagaat tagccagagg cgcacagttg aatatcggcc tgccggagaa aactactgag
     6421 tgtctgggga cataatttct cctgtgccgt gagcataatt actcatgcga cataatgccg
     6481 gtaacagcga ttctaaaccc ttttggtaag tactaacacg ctcctgaaaa ggagctaaaa
     6541 cagatgcctg agaacgaaga tagaatcaaa ctgcgagaaa gccacagaac cgggttaact
     6601 cccacaaaaa atggtcttcg cctcgaccgc aaatcattta cgcgatgtac acgtctgcta
     6661 tacagactca tagcccattg taacgggcgc tacaatattg cccgaaaccg ggccgatgcg
     6721 cggcatcaaa aggagataac atggtcaaaa gactgccacg actgctacct ttcactgaag
     6781 agcacgaccg cttccgcgaa atggttgctg acttcatcgc gaaagaatgc gtaccacgcc
     6841 acgaagaatg ggaaaaagcc ggcgaagtgt cgcgcgacgt ttgggaaaaa gccggacaac
     6901 tcggcatgat ctgcccgaac tttcccgaag aatatggcgg catgggcctc gactttctct
     6961 ataacgtgat cgtcatcgaa gagctggcta aggctgcatc ttctggcttc tttatctcgc
     7021 tgcacgccga tgtgatcgcg ccctacgttc tgcactatgc caacgaagaa caaaagaagc
     7081 gctggctgcc gggtattatc aacggtacca aaattctctc gatcggtatg actgaaccgg
     7141 gcacaggctc agacctcgcc gcggtgacga ccaaggctgt cgacatgggt gaccactacc
     7201 tgctcaacgg ttcaaagaca ttcatttcga atggttacct ctcagacctc tgcatcgtgg
     7261 tagcgcgtac tggcgaaggc cagggaggca tctctctgct gatggttgag cgcggtatgc
     7321 caggttttga acgcggccgc aaactgaaga agatcggtct gcatgcgcag gacacctcag
     7381 agctccactt taacgatgta aaggtgccaa aagcaaacct gatcggtaaa ctcaacgcag
     7441 gtttccgtta cctgatgatg tcgctcgcgc aggaacgcct cgtgcttgcg atttcgaaca
     7501 tcgcttcagc cgaaacagtg ctggatcaga ccgtgaagta ctgcaacgag cgtaaggtct
     7561 ttggtaaacc cgtcggttca ttccagaaca cacgcttcag cctgaccgat atgtacgtcg
     7621 agcaggaagc tgcacgctct tactgcgacc gcgtcgtcat ggccttcatg gcaggcgaaa
     7681 aagtgactgc agaagcttct gccgtgaaac tgcagtgctc tgaaatgctg caggcgcacg
     7741 tcacgaagtg cctccagttc tttggcggtt acggcttcat gatggaatac ccgatcgcca
     7801 aggcatacct cgactgccgc gtgcagacga tctatgcagg tacatcagaa atcatgcgcg
     7861 agatcgtatc gaagaacctc ggcctcgcca gcacctgacg acatggtgag cagggtgcca
     7921 gcagccaaag gcggtcgcag gcacctaatt tccctcaacc gatggggaat actctccagg
     7981 tatttcctgt cgggcctctt tctcctctcc ctctttcact gtacctcaga gcacaatacg
     8041 gtacgcctct acccggccaa gagtgataag tcgtcgtacc ggcagctctc taccgaacac
     8101 tacctgtacg t
//
LOCUS       DAOQJF010000128         8083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4819_length_8083_cov_4.627803, whole genome shotgun sequence.
ACCESSION   DAOQJF010000128 DAOQJF010000000
VERSION     DAOQJF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4819_length_8083_cov_4.627803"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..673)
                     /gene="efp"
                     /locus_tag="PLF85_08180"
     CDS             complement(107..673)
                     /gene="efp"
                     /locus_tag="PLF85_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNE19681.1"
                     /translation="MINSNHIKRGMALKVDGDLVVIQSFEHHKPGKGAAIVRVRIKSL
                     SKGTTVERTWRSGEMLEDVELEKRNVTYNFEDGDQLVFMDSETYDQIHVDKKDLEDIL
                     QFITDGMEFQILLYEGKPVSVIPPNFIVAKVEYAEEGLKGDTATTAYKEVTVEGGGKI
                     QTPLFVKTGDMIKIDLRDMSYVERVNQK"
     gene            978..1136
                     /gene="rpmG"
                     /locus_tag="PLF85_08185"
     CDS             978..1136
                     /gene="rpmG"
                     /locus_tag="PLF85_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNE19682.1"
                     /translation="MREIVKLESTAGTGFFYTTTRNKKAQGGKLEVMKYDPKIRKHVK
                     FVEKKASK"
     gene            complement(1164..2699)
                     /locus_tag="PLF85_08190"
     CDS             complement(1164..2699)
                     /locus_tag="PLF85_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE19683.1"
                     /translation="MRRVIAATLLTPIVLFAEVKITPLGNGVNSRYDELAPVLAPDGN
                     TIFFCREGHPDNVGVRTIVDGASALRDDQDIWMSSRKADGSWSEAQHIAAPFNSRTYD
                     FPIGTSADGKTLYIGNVYGKDGTVSPGVSKTRLRGGKWSFPEPLRIQNFYNEANLVNY
                     SLSADERTLILNLQRKDTVGRMDLYVSFLQPDDSWSEPMNLGKEINSAFMEVTPFLAS
                     DNKTIYFASNRPGGVGQFDMYMSRRQDNTWTHWTEPVNLGPNINTTGNDINYVIAPTG
                     DYAIFASDTPGKGKDLYRISLPEELRPEKSILVFGKVVGKDGKGVQGRVVFERLSDGA
                     LLARTETNEDGDFKMNLPLGEVYGIHAESGGYLPQSTTIDLQKGFSKEHPIELNMTQL
                     APGAKMPINNIFFRKGSYQLSKESNAELNRLTKLLKGYPGLKVEIGGHTDNTGDDRSN
                     MELSKNRARAVMDYLIEKGMPRNSIKAVGYGESRPIASNNTPAGKKKNRRVELTILSI
                     DKK"
     gene            complement(2757..3797)
                     /locus_tag="PLF85_08195"
     CDS             complement(2757..3797)
                     /locus_tag="PLF85_08195"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HNE19684.1"
                     /translation="MKPRIFRVCLKGVLTGIWLVLACIKTDTPSLLGLEFVDSVRVQN
                     VQITTLDTTSVGIRWEALPPGKSTIELSTNAVFSSIAFSSGPITETSHRFTGLVVNTT
                     YFYRVRTISPSGKVSEYAPNPVGSFTVPVDMNPDLPRTSCLELHQLFPSLPSGTYSLD
                     TDGVGSNAPFQAYCDMTLSDGATSGGWTLILVYNDSQGTILSSDTPAAVVTPGAGKKE
                     FMAYSRMAPLSQNGTQIALRERGSATNYIISRPGSPQIRLLSYAGVGSAVNIFPGMNA
                     PAKIDLAWIRGSGISTARLTGTCVPQDSPYPENLHQTCGNGVGFHFIPSLGYANWNTS
                     GASGVDFEVLIR"
     gene            4108..4674
                     /locus_tag="PLF85_08200"
     CDS             4108..4674
                     /locus_tag="PLF85_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE19685.1"
                     /translation="MIFIFNLALFVVLERVYVLRKVRGDSAGLVQYLTEALHRGESID
                     KIAAEAAEAKYGLEGRIAAVSLKGWDKGEHAMKEYAESALIAERRGLEKRLVILSTLG
                     NNTPFIGLLGTVLGIMKAFRDLAEMGDSGPSVVMRGISEALIATAMGLGVAIPVVIAY
                     NALSRVVQDKMSQSEEICTLIRAIRLNK"
     gene            4676..5098
                     /locus_tag="PLF85_08205"
     CDS             4676..5098
                     /locus_tag="PLF85_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNE19686.1"
                     /translation="MAGSTKGNGSGPISDINVTPLVDIMLVLVIILMVTAEFTRYKTI
                     PVALPKVNAAAVKAEPHRLTVTIKKTGDLFVQDKPAMSLDDIYDQVKVAKETYPDLAV
                     IVRAEGDHPYRKVLTVLDKIKAAGASKVGLAVEADKTY"
     gene            5107..6321
                     /locus_tag="PLF85_08210"
     CDS             5107..6321
                     /locus_tag="PLF85_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19687.1"
                     /translation="MPSDARIFLSEIRPQHWFETVPARVSFSLLLLVFLAIPTFLLLV
                     NDARLLLYYSIYPLTALFSVYLRYKGREYLSSLVMVLSVYGVVLWMCWLIGFAQVNEL
                     KLWIIALLPWVMFGESHRLSLAVLSLVPVTFSLTLRFLPEIHSPLFPAEREFVAQMLK
                     ISVGMGAFSCVYYLRQQFTTAEKKRILENEFYSNTLNNIPLPIIIKDGITLDYIFYNS
                     AAALTFDLKPGYRHSNQTTFSETCAAAVSRIDHDVLRSVTYHIESDENLVHQTGLTWH
                     FRTYRIPLELRSSGRRLLITVSEDLRAINILLRKAEETHSTLASIVSLVSPLLIRFDR
                     RGQKVHTVQSGTGDLPSDILAYLEFFFSRNQAQATGGQHSFELGNQRFLLCYGPVPRQ
                     EDLHGVVIALAG"
     gene            6381..6890
                     /locus_tag="PLF85_08215"
     CDS             6381..6890
                     /locus_tag="PLF85_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19688.1"
                     /translation="MLDVGEEIRIQKNSGPTSLFLHIPYLNYVFFFFQGRGAYNIVKN
                     LYQYKKEVPGLFTSAWLTAKFAILSTIASTLLYSLLLALGYNPFGKVLAKYIYFFGNI
                     LGGMVPANYLALVFGEVPGDLLFASFSSGTYIALVSITFATYFLFGMAGAMLAIFNIK
                     ADIRRAQYF"
     gene            6911..7537
                     /locus_tag="PLF85_08220"
     CDS             6911..7537
                     /locus_tag="PLF85_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE19689.1"
                     /translation="MAMTIDGHVAHPTDIWNYGSSEDRRRMDRLREWAQCLIVSRKTL
                     EHDNMDLRVRTKLRAKHPRPVIVMQSPRPLRKGLNAAKYAATPGELWLSAAHQAPAVS
                     ELWSEIGADWQIHQHKQVDEIVKSLRQRGYERLLLEGGPSLNGLFFEHDLVDEFYLTI
                     LPIAWGGTTTDRSIVSSRPLPLQRFRLQSAEKRKNEMFLRYIRRRLPG"
     gene            complement(7573..>8083)
                     /locus_tag="PLF85_08225"
     CDS             complement(7573..>8083)
                     /locus_tag="PLF85_08225"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNE19690.1"
                     /translation="IDQHTAHERINYERFLDLLAGKKDISQRLVQPLPVSAALFERTS
                     LRQAGKSLTAMGFLLEDLGPAGHALRAVPGYIDPGEEQAALETALRLIEHSDLSEASD
                     LFDQLAKDLSCRHAIRKGESASMVDFRELVDRLRSCKNPMRCPHGRPTLVKIDEREVF
                     AFFKRQAGN"
BASE COUNT         1829 a         2276 c         2085 g         1893 t
ORIGIN      
        1 gcaacagaac ccgcctcggt aaaatgcccg ccgcggaaca gctcaaagcc agcggctaaa
       61 ttggccatac cctgtacgac caatcaaaag taattgccgc aggcaattac ttttgattca
      121 ctcgctcgac gtagctcatg tcgcgcaggt cgatcttgat catgtcgcct gtcttgacaa
      181 agagcggagt ttgtatcttg ccgccgcctt ctacggtgac ttctttgtaa gcagttgtgg
      241 cagtatcgcc tttcagaccc tcttcagcgt attcaacctt ggcgacaata aagttgggcg
      301 gtataacgct cacgggctta ccctcataga gcagaatctg gaactccatg ccatcggtga
      361 taaactgcag aatgtcttcg agatctttct tatcgacgtg aatctggtcg taagtttcgc
      421 tgtccatgaa gaccagctgg tcaccgtctt cgaaattgta ggttacgttt cgcttttcga
      481 gttcgacatc ttcgagcatc tcgccagacc gccaggttct ttctaccgtg gtgcctttac
      541 tgagcgattt aatgcgcacg cgcacgatgg ctgcaccctt gccgggctta tggtgctcga
      601 agctctggat aacaacaagg tcaccgtcaa ccttgagtgc catgccacgc ttgatgtggt
      661 tcgagttaat cattatgttt gatttctgtt ggacggctac gccgtccaac agaaatcaaa
      721 taaaaaaacc cgtttgccct gctggatttt tttctttcag agcaaacggg tgagaagacg
      781 cacgacccgc gggtttttcg cccgaagacc gctaaacctc cactgggggg aggtctccaa
      841 tatcagtttc ggcttgaggg gggctggact tgagtgggtt tttctgccgg ttaaccagcc
      901 tcagtacccc ggggctggca gtgcacgcac tcgtccaaat gcggtttaca ccctgttcaa
      961 attatggccg gtgccctatg cgcgaaatcg tcaaactcga atcgacagcc ggcacgggct
     1021 tcttttacac cactacccgc aacaagaagg cgcagggtgg caagctcgag gtcatgaaat
     1081 acgacccaaa aatccgcaag cacgtaaaat tcgtcgagaa aaaagcttct aaataacagt
     1141 tttgcggggg gtaccctgca aaactatttt ttgtcaatcg acagaatcgt cagctctacg
     1201 cggcgattct tcttctttcc tgccggtgta ttgttcgaag ctatcgggcg tgactcgcca
     1261 taacccacgg ccttgatcga attgcgcggc atgccctttt caatcaggta atccatcacc
     1321 gcgcgtgcgc ggttcttcga gagctccatg ttgctgcgat catcacctgt gttgtcggta
     1381 tgaccgccga tctctacctt gagccctggg taacctttca aaagctttgt cagccggttg
     1441 agctctgcat tcgattcttt cgacaactgg tatgacccct tgcgaaaaaa gatattgttg
     1501 atcggcatct ttgcgcccgg cgccagctgc gtcatgttga gttcaatggg gtgttccttg
     1561 ctaaatccct tttgcaggtc tattgtcgtg ctctgcggca ggtaaccacc cgactcggca
     1621 tgaatgccgt agacctcgcc gagcggcagg ttcattttga aatccccgtc ttcgttggtc
     1681 tcggtacgcg caagcaaggc accgtcgctg aggcgctcaa acaccaccct gccctgcact
     1741 cccttaccgt ctttgccgac gaccttgccg aatacaagta tcgatttttc cggccgcagc
     1801 tcttctggca gcgaaatgcg gtagaggtct ttacctttgc ccggcgtatc ggacgcgaag
     1861 attgcgtagt cgccggtggg agctatcaca taattaatat cattacccgt cgtgttaata
     1921 ttcggcccca ggtttaccgg ctctgtccag tgcgtccagg tattgtcctg ccgccgcgac
     1981 atgtacatgt caaactgccc caccccgccc gggcggttcg atgcaaagta gatcgtcttg
     2041 ttatccgaag cgaggaatgg agtaacttcc ataaatgcgg agttaatctc tttaccgagg
     2101 ttcattggct cagaccatga atcgtccggc tgcaaaaaac tcacgtagag gtccatacgg
     2161 cccactgtgt ctttgcgctg caggttgaga atcagcgtgc gctcgtcggc cgaaagcgaa
     2221 tagttcacaa ggttcgcctc gttataaaaa ttctgtatgc gcagcggttc ggggaaagac
     2281 catttgccac cccgcaggcg ggttttcgag acacccggcg agactgtgcc gtcttttcca
     2341 tagacattac cgatatagag cgtcttgccg tcagccgaag tcccgatcgg aaaatcgtac
     2401 gtccgtgaat taaacggcgc cgcgatgtgc tgcgcctctg accacgaacc atctgccttg
     2461 cggctgctca tccagatatc ctggtcgtcg cgcagcgcgc tggcgccatc gacgatcgtc
     2521 cgcactccca cattgtccgg gtgcccttcg cggcagaaga agatcgtatt gccgtcggga
     2581 gccagcaccg gcgcgagttc atcgtagcgc gagttgactc cgttacccag gggggtgatc
     2641 tttacctcgg cgaacagaac tatgggggta aggagcgtgg cagcgataac tcggcgcata
     2701 gtgtataatc ggaacaaaga ctcgcgtcgt cgttcaaaaa acgagcggat agcgaattac
     2761 cttatcaaga cttcaaagtc aactccgctc gccccgcttg tgttccaatt ggcataaccc
     2821 aaagatggaa taaaatggaa gccgacgcca ttgccgcagg tttgatgcag attttcaggg
     2881 taaggcgaat cttgcgggac gcaggttccc gtcaggcgtg ccgtcgaaat gccgcttccg
     2941 cgaatccatg ccagatcgat cttggctgga gcattcattc ctggaaaaat attcacggca
     3001 gaaccaaccc cggcataact cagcaagcgt atctggggac taccaggtct gctgataatg
     3061 tagttggtcg cggagccgcg ctcgcgaagc gcaatctggg tgccattttg cgatagcggc
     3121 gccatgcgtg aatacgccat gaactccttc ttaccggcac ctggcgtcac tacagccgca
     3181 ggggtatctg aactcaggat agtcccctga ctatcattgt acacaaggat aagcgtccat
     3241 ccgccggacg tcgcgccatc agaaagcgtc atgtcgcaat atgcctgaaa cggcgcattc
     3301 gaacccaccc catccgtatc aagggaatac gtgcccgaag gcaacgacgg aaataactgg
     3361 tgcagctcta gacagcttgt acgcggtaaa tcggggttca tgtcgacggg gacagtaaac
     3421 gagcccacgg ggttgggtgc atattcactg accttgccgg acggacttat cgtacgcacc
     3481 cggtaaaagt acgtcgtatt gacgacgagt ccggtgaatc gatgcgaggt ctcggtgatt
     3541 ggccccgaag aaaacgcaat cgacgaaaat accgcattag tgctcagttc gattgtactc
     3601 ttgcccggtg gaagcgcctc ccaccttatg ccgaccgaag ttgtatcgag tgtcgtaatt
     3661 tgaacattct gaacacgcac cgaatcgacg aattccagcc ccagcagcga cggtgtgtcg
     3721 gtcttgatgc aggctaaaac caaccaaatc cctgtcaaca ccccctttaa acatacgcga
     3781 aatatccgag gcttcatgtg tctggtttca gcgcagggcc attgggtcaa cattgaattg
     3841 tccttgcctg tcggtagtga aaactttacg ccaccattgg ctcttccttt ctgccgtatg
     3901 cgtatgcgga tcttcctatt tgcttcactc tttgcgctct cgggcagctg gtttatcagc
     3961 gcccagcaaa aagcggcacc ggcaacacca gccgcaactg cgcctgctgc acccgtagcg
     4021 aatgccgaac ctgcacaggc aggcggcggt aacatgctcg cggaagctgg tcgctggcac
     4081 tttggctctg acccgaccgt ttgggtaatg atcttcatct tcaacctggc gctcttcgtt
     4141 gttctcgagc gcgtctatgt gctgcgcaaa gtacgcggcg acagcgctgg cctcgtgcag
     4201 tatctcacag aggcgctgca ccgcggcgag agcatcgata agatcgctgc tgaagcagca
     4261 gaggcaaagt atggcctcga aggccgtatc gccgcggtat cgctcaaagg ctgggacaag
     4321 ggcgaacacg cgatgaaaga atatgcagaa tcagcgctca ttgcagaacg ccgcggtctg
     4381 gagaaacggc tcgttatcct ctcgacactc ggcaacaata caccgttcat cggtctacta
     4441 ggtacggttc tcggtatcat gaaagcgttt cgtgatcttg ccgaaatggg cgattcaggg
     4501 ccttcggtgg tcatgcgtgg tatctccgag gcgttgattg caacggcaat gggtctcggg
     4561 gttgcaattc cagtggtcat cgcctacaat gccctctcgc gcgtcgtgca ggataaaatg
     4621 tcacagtccg aagagatctg caccctgatt cgcgccattc gcctcaacaa gtaacatggc
     4681 aggctcgacc aaaggcaacg gctcaggccc gatcagcgac atcaacgtca cgccgctggt
     4741 cgacattatg ctcgtgctgg tgatcatact catggtcact gcggagttta cccgatacaa
     4801 gactatcccc gtggcgctgc caaaggtgaa tgctgccgcg gtcaaagctg agccgcaccg
     4861 gctcacagtg accatcaaaa agaccgggga cctgtttgtg caggataaac cggccatgag
     4921 tctcgatgat atatacgacc aggttaaggt ggcgaaagag acatatccag atcttgccgt
     4981 aattgtccgc gcagaggggg atcatcccta ccgcaaggta ctcacggtac tggacaagat
     5041 taaagcagcc ggtgcttcca aagtaggact tgccgtagaa gcagacaaaa cttactagtc
     5101 cgcacgatgc cctcggacgc gcgcatcttt ctttcggaga tccggcccca acactggttt
     5161 gaaacagtac ccgctcgggt aagtttttcg cttctcttac tggtttttct cgcaatccct
     5221 acttttctcc tgctggttaa cgatgcgcgt cttttactct actattcaat ctacccgcta
     5281 actgcacttt tttccgttta cctgcgctac aagggcagag agtacctcag cagccttgtc
     5341 atggtcctga gcgtttacgg cgtcgtcctc tggatgtgct ggctgattgg ttttgcacag
     5401 gttaatgagc tcaagctctg gatcatagca ctgttaccct gggtcatgtt tggtgagagc
     5461 catcggctat ccctggcggt actaagcctg gtaccggtca cgttttcact gaccctgaga
     5521 tttctgcccg aaatccacag cccgttattt ccggcagagc gtgaattcgt ggcacagatg
     5581 ctgaaaattt ccgttggaat gggtgctttc agctgtgtat actatctgag gcaacaattc
     5641 acaacggctg agaagaagag aatcctcgaa aatgaattct actccaacac gctgaacaat
     5701 attccgctgc cgataattat taaagacggc atcacgctcg attacatctt ctacaacagt
     5761 gccgcagccc tgacatttga cctgaaaccc ggttaccggc attcaaacca gaccacgttt
     5821 tcagaaacct gcgccgctgc ggtctcgcgt attgaccacg acgtactgcg cagtgtcacg
     5881 taccatattg aatcggacga aaatctcgta caccagacgg gcttaacctg gcatttcagg
     5941 acctaccgca ttccgctcga actgcgcagc agcggacgcc ggctgctgat tacggtaagc
     6001 gaagacctaa gggccatcaa catactgctg cgcaaggccg aagaaacaca ttcaacgctt
     6061 gccagtattg tgtcgctggt ttcgcccctg ctcatccgct ttgaccgcag aggccagaaa
     6121 gtacacacgg tgcagtctgg tacaggggac ctaccttcag acatactggc gtacctggag
     6181 ttcttcttct cgcgcaacca ggcgcaggct accggcggcc agcacagctt tgaactagga
     6241 aaccagcgtt ttctcctctg ttatggacca gtaccccgcc aggaagattt gcatggcgtg
     6301 gtgattgcac tggccggctg agccagctgt gtatgaacga taattcattg acagtcagcc
     6361 ctgaccgctg actcgtcccc atgctcgacg tcggcgaaga gatccgcatc cagaaaaact
     6421 ccggtcctac cagtcttttt ctgcatatcc cctatctcaa ctatgttttc ttctttttcc
     6481 agggacgtgg agcttacaat atcgttaaga atctctacca gtacaagaaa gaagtaccgg
     6541 ggctcttcac ttctgcgtgg ctgaccgcta agttcgcgat actgagcaca atcgcgtcga
     6601 cactgctcta cagtctgctg ctggcgttgg gctacaatcc atttggcaag gtactggcaa
     6661 aatatatcta tttctttggt aatattctgg gcggcatggt tccagccaat tatctggcac
     6721 tagttttcgg cgaagttccg ggagatctgc tttttgcctc gtttagcagc ggtacctaca
     6781 tcgccctggt ttccataacc tttgccacgt atttcctctt cggcatggca ggcgcgatgc
     6841 tggccatttt caatatcaag gctgacatcc gtcgcgccca gtacttctga aatccctgct
     6901 caagctcaac atggccatga cgatcgatgg ccatgtagct caccccaccg acatctggaa
     6961 ctacggcagc agcgaagacc ggcgccgcat ggaccggctg cgcgaatggg cacaatgcct
     7021 gattgtttca cgcaaaaccc ttgaacacga taacatggac ctgcgggtac ggacaaaact
     7081 ccgtgcaaaa cacccgcggc cggtgattgt catgcagtca ccccggcccc tgcgcaaagg
     7141 tctcaacgcg gctaaatatg ctgcaacgcc tggggaactc tggctcagcg cagcacacca
     7201 ggccccggct gtgagcgaac tatggagtga aattggcgct gactggcaaa tacaccagca
     7261 caaacaggta gatgaaatcg tcaaatcgct gcgccagcgc ggctatgaac gcttactgct
     7321 cgaaggtggt ccgagtctga atggcctctt cttcgagcac gacctggtcg acgaatttta
     7381 tcttaccata ttaccgatcg catggggcgg cacgacgaca gaccggtcta tcgtcagtag
     7441 ccggccacta ccgctgcaga ggttccgtct gcagagcgca gaaaagcgga agaacgaaat
     7501 gttcctgcgc tacatccgcc gcaggttgcc tggctaaccg gggcgcccac gcaccacccg
     7561 ctgataaaaa ccttaattac ctgcctgtcg cttgaagaag gcaaatactt cgcgctcgtc
     7621 gattttgacg agcgtcggcc tgccgtgcgg gcagcgcatg ggatttttgc agctacgcag
     7681 ccggtctaca agttcgcgaa aatctaccat agaggcgctc tcgcctttac ggattgcatg
     7741 acggcaggac agatctttcg ccagctggtc aaagagatcg ctggcctcgc tgaggtcaga
     7801 gtgttcaatg agtcgcaagg cagtctcgag cgccgcctgc tcttctcccg gatcaatata
     7861 tcccggtact gcgcgcaaag cgtgaccggc cggtcccaga tcctcgagca ggaagcccat
     7921 ggccgtaagc gactttcctg cctgcctgag gctggtgcgt tcaaaaagcg ctgctgaaac
     7981 cggcaagggc tgcacgaggc gctgcgaaat atctttttta cccgcgagca aatccaggaa
     8041 tcgctcgtag ttgatacgct cgtgcgccgt atgctggtcg atg
//
LOCUS       DAOQJF010000129         8015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4876_length_8015_cov_5.340201, whole genome shotgun sequence.
ACCESSION   DAOQJF010000129 DAOQJF010000000
VERSION     DAOQJF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4876_length_8015_cov_5.340201"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLF85_08230"
     CDS             <1..544
                     /locus_tag="PLF85_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE19691.1"
                     /translation="GKIIGGGLPVGAIAGSAAIMDNLAPQGPVYQAGTLSGNPLAMAA
                     GQAALACLAERNGAVYSHLKKLADRLKEGFEKEILPLLAGREWTIQLVQEESLFWFSF
                     RPQGDNSPVRRVDRIAPFSANIYKQIFHAMLVRGVYLAPSAYEVGFLNAAMRGRDIDY
                     FIDNLRKTVRQLSGSIKDFT"
     gene            541..1608
                     /locus_tag="PLF85_08235"
     CDS             541..1608
                     /locus_tag="PLF85_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE19692.1"
                     /translation="MSVSRQKKNLSGFSFFSGSALAMAFSLLSVIGLLLWWQSLITRH
                     LEIQYRFFSTQVTSGAPVSEDLLRLIRDQDERLARELFPQAENNLAPANAREIIQARL
                     ANRRKMVIYERTFFILLLFSGHLFFLYIYFRERQRRKLTEETILLATHELRQPLQSLS
                     LALETVQDDARGTSKAAISAGLTDINRLGHHIRWLASTFAETKHRNKQTELTSIASWF
                     GDLMREEFSERERQRLQIKLPDEAVLLCIEENLLRFVLRNLTENALRYAQGQVEISGQ
                     TERRRVRFTVANAGARMQRGDFRRLGNLFFRSPAADIQNKSGFGLGLYLCGRIARRAR
                     GRLTLVNDGDRLVAELVVRKK"
     gene            1605..2300
                     /locus_tag="PLF85_08240"
     CDS             1605..2300
                     /locus_tag="PLF85_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE19693.1"
                     /translation="MSNRNKHIAIVEDDDNIRELLAENLRRAGYQVSAFFTAEQFERK
                     SDAGYHMLLLDIMLPGKSGITLLKDLKSHGLDFPVILLTASEQDAHVDAAFGSGAIDY
                     IIKPFNLKHLLQKIENLFEQLKPAGMGENRSDYAIGAGRFEPELNIVIREKKEYKLTP
                     AEAATLLYFIRNPNRIISREELQASLGDENSSLTSRNLDNYVLKFRKLFETDPKSPLL
                     FVTIPRKGYALKR"
     gene            2306..3331
                     /gene="hemE"
                     /locus_tag="PLF85_08245"
     CDS             2306..3331
                     /gene="hemE"
                     /locus_tag="PLF85_08245"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNE19694.1"
                     /translation="MENHNNRFDRAVRGLPVDRTPIWFMRQAGRYLPEYREVRKRLSF
                     DQMLADADVATEVTLQPIKRFDLDAAVIFADIMTILNGLDIPFNFASGGPALEFHIQN
                     PKERAKLDARRLQENAGRLEPFLRAIRQTRAVLPAEKSVLGFAASPFTLLSYLIEGTT
                     SKTHNTARAFMLGSALEFHSLMEKVADCTIEYLSLQVAAGASAVQLFESWGDVLSPRT
                     YGEQILPHVEKIVNAISPLAPVIVYDKGASLHLPVLRPFIQRSGAVLSVDWRVSIAEL
                     GDLKIQGNLDPAALESEPSAVRRETARILAERAGKPGHVFNLGHGISPQAKLECVEAM
                     VETVQSS"
     gene            complement(3972..5309)
                     /locus_tag="PLF85_08250"
     CDS             complement(3972..5309)
                     /locus_tag="PLF85_08250"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19695.1"
                     /translation="MNIRRFRPGAFVELECRHFVAEGLGLSYFADESLKEKFKPHVFF
                     IWGVLPGERFLARLAQVKSKVAHGVLARIDELPAEFVGEGNFAHETWALRNLAEDRGK
                     PACDHYLRCGGCRMQHMSYERTLYYKQAWLQSQLDRNKVEVPAMEFHSLPEDTRWHYR
                     NHVQVHINKFGTYGFYEPVSYRTRAFPEHGCLIFDEKSLREKFPRFPVEVRAARIRQN
                     TDGEVVFSVLNSKEESQSIARYSVAWPAGREISIHFPVTGFFQANLRALPYWLTTIAQ
                     YFARTGISNPRVLELFSGFGFISRMQSLIHPMQVMALDILNPDQVRQVRFEEHGHPLP
                     DHFAERYRKVDLFLPERIRDDLLAEIRAFRPEILLINPPRAGLVPETWKRLRAEALGE
                     FSGPIIYSSCDAATMARDLAAFAEAGYRCEQMEVFDFFPWTHHFETVSYLARA"
     gene            complement(5352..6902)
                     /gene="purH"
                     /locus_tag="PLF85_08255"
     CDS             complement(5352..6902)
                     /gene="purH"
                     /locus_tag="PLF85_08255"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNE19696.1"
                     /translation="MNNLVPIRRALISVSDKSGLKEFASTLHSLGVEIYSTGGTLKYL
                     REQGLPALAVEDYTGFPEMMDGRVKTLHPKIHGGFLARRDLESHLEAARQHDIKLFDL
                     VVINLYPFEAVTAKAGTTFEEAVENIDIGGPAMVRSAAKNAASVAIVVSPEQYAAVAA
                     ELEANKGATSLALREELRLAAYRRTAAYDAAISAYLEEKSGVAGMRLSLVEKQGLRYG
                     ENPHQNASYLVEAGKDVPWKQLAGKELSFNNILDLDIAIRLAADFSEPVCAIFKHTNP
                     CGVATGNDQAQILQHAMDCDPVSFFGGIVVFNRPLGKAAAEKLAPHFFELIIAPEFIE
                     GAFEILAAKKNLRLITVDFGKLDAAKTRDLRSVPGAGGYLLQDQDLPLWNEADIKVVS
                     KAQCDDQTMKDLAFASRVAKFVKSNSVVFASGGRSLAIGAGQMSRIDSIRIAQEKAAD
                     AKLSLKGSVMASEAFFPFRDSVDTCAKIGVRAIVQPGGSIKDAESITAANEHGIALLF
                     SGMRHFRH"
     gene            complement(6949..7818)
                     /gene="dapF"
                     /locus_tag="PLF85_08260"
     CDS             complement(6949..7818)
                     /gene="dapF"
                     /locus_tag="PLF85_08260"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNE19697.1"
                     /translation="MSGSKALRFAKLEGLGNDYLFTEDSPKNPQRAARLLCDRHYGVG
                     ADGLVLIMPPDNKNEADFQIRIFNPDGSEAEMCGNAIRCLGKYLYDRGNTKKQEINVQ
                     TLRGIIPCILMVRDGRVYRVKADMGEPILERAKIPMVCAAGEEEKKVLKEKIYLEDHN
                     TFEFMALSMGNPHVVIWVDDVDNFPVQKYGPMIENYSLFPNRTNVHFIQMVDEDHIKQ
                     RVWERGVGETLASGTGACAAVVAGVLSGKIKRKAKVELRGGILEIYWSEKDNHVYMTG
                     PCKDVFEGQTMLI"
BASE COUNT         1834 a         2244 c         2162 g         1775 t
ORIGIN      
        1 tggaaaaatt atcggcggcg gtctgcctgt gggtgccatt gcaggcagcg ctgccatcat
       61 ggacaatctt gccccgcagg gccctgtcta ccaggcgggc acgctctctg gcaatcctct
      121 tgccatggca gcaggccagg ctgccctcgc ctgcctcgcc gaacgcaatg gtgcagtata
      181 ctcccacctg aaaaagctcg ctgacaggct caaggaaggc tttgaaaaag aaatcctgcc
      241 gctactcgca ggtcgcgaat ggacaatcca gctggtgcag gaggagtcac tgttctggtt
      301 ctctttcagg ccgcagggtg acaatagccc ggtaaggcga gtcgaccgga ttgccccctt
      361 ctctgccaac atctataaac agattttcca cgcaatgctt gtccgcggcg tctaccttgc
      421 gccgtcggcc tacgaagtgg gatttctgaa tgctgccatg cgcgggcgcg atattgacta
      481 ttttatcgat aacctgcgca agaccgtgcg ccagctctcg ggttcgatca aagattttac
      541 atgagcgtca gccggcagaa aaagaacctc tccggatttt cttttttttc cggttctgcc
      601 ctcgcgatgg cattttcgct gctgtcggtt atcggcctgc tcttgtggtg gcagagtctc
      661 atcacccgcc acctggaaat ccagtaccgc ttcttctcta cgcaggtaac cagtggcgcg
      721 ccggtctctg aagacctgct gcggcttatc cgcgaccagg acgaaaggct ggcgcgcgaa
      781 ctgtttccgc aagccgaaaa caacctggcg ccagccaatg cccgcgaaat catccaggcg
      841 cgcctggcaa accgcaggaa gatggtcatc tacgagcgta ctttttttat cctgctcttg
      901 ttctccgggc acctcttttt tctctacatc tactttcgcg aacggcagcg gcgcaaactg
      961 accgaagaaa cgatactgct ggcaacgcat gaactccgcc agccgctaca gagcctgtcg
     1021 ctcgcgctcg agactgttca ggatgatgcg cgcggaacca gcaaagccgc gatcagcgcg
     1081 ggcttaacag acatcaaccg gctgggccac catatccgct ggctggcttc aacttttgcc
     1141 gaaaccaaac accggaataa gcagaccgaa ttgaccagca tagcctcgtg gtttggtgac
     1201 ctgatgcggg aagagttcag cgaacgcgag cgccagcgat tgcagattaa gctgcctgat
     1261 gaagccgtgc tcctgtgcat tgaagaaaac ctgctgcgtt ttgtcctgcg caacctgact
     1321 gaaaatgcgc tgcgttacgc gcagggccaa gtggaaattt cgggacagac agaacgcaga
     1381 cgggttcggt tcacagtagc aaacgctggc gcccgcatgc agcggggcga ttttcgtcga
     1441 ctgggtaacc tgttcttccg ttcgcctgcg gccgacatcc agaacaagtc gggcttcgga
     1501 ctcggccttt acctctgcgg cagaatcgcc cggcgggcgc gcgggcgcct tacactcgtg
     1561 aatgacggcg atcgtcttgt tgctgaactg gtcgtgagaa agaaatgagt aaccgcaata
     1621 aacatatcgc gattgttgag gacgacgaca atatcaggga attgctcgct gaaaacctgc
     1681 gccgcgccgg ctaccaggtc tcggcatttt ttaccgcgga acagtttgaa agaaaatccg
     1741 atgcaggtta ccatatgctg ctactggaca tcatgctgcc gggcaagagc ggcatcacgc
     1801 tgctgaagga tctcaagtcg catggcctgg attttcccgt gattctgctc accgcttcag
     1861 agcaggatgc gcacgtcgat gccgctttcg gttccggggc gattgactat atcatcaaac
     1921 cctttaacct gaagcacctt ctgcaaaaaa ttgagaacct gtttgaacag ctaaagccgg
     1981 ctggaatggg ggaaaatcgc agcgactacg ctatcggggc cggccggttc gagcccgaac
     2041 tgaatatcgt cattcgcgaa aagaaagaat acaagctgac gccggcagaa gccgcgacgc
     2101 tgctctactt catcaggaac cccaaccgga tcatttcgcg cgaagaactg caggccagcc
     2161 tgggcgatga gaatagcagt ctcacctcgc gcaatctgga taactacgtg ctcaaattcc
     2221 ggaagttgtt tgagaccgat ccgaaatccc ccctactctt tgtaacaata ccgcgaaaag
     2281 gatatgcgct gaagcgctga tagctatgga aaaccacaac aaccgatttg atcgcgcagt
     2341 acgcggtctg ccagtcgacc ggacgccgat ttggtttatg cgacaggccg ggcgctacct
     2401 gcccgaatac cgtgaagtgc gcaagcgcct cagctttgac cagatgctgg ccgacgccga
     2461 tgtcgcgacc gaggtaacac tgcaaccaat caagcggttt gacctggacg cggctgtgat
     2521 atttgccgat atcatgacca tactgaacgg tctggatatt ccgtttaatt ttgcttccgg
     2581 ggggcctgct ctggaattcc atatccagaa tcctaaagag cgggcaaaac tggatgcgcg
     2641 ccggctgcag gagaatgcag gccgcctcga gccattctta cgtgcgattc gccagacgcg
     2701 ggccgtcctg ccggctgaaa agtcggtgct cggctttgcg gcctcaccat tcaccctgct
     2761 gagctacctg attgagggga cgacatcgaa gactcataat acggccaggg cttttatgct
     2821 gggctctgct ctggaattcc atagcctgat ggagaaggta gcagactgca ccatcgagta
     2881 cctgagcctg caggtagccg caggtgcttc tgccgtgcaa ctctttgaat cctggggtga
     2941 tgtgctttct ccgcgcacgt acggagagca gattttgccc catgtagaaa agatcgtcaa
     3001 cgccatcagt ccgctcgcgc ctgtcatcgt ctatgataag ggtgcctcac tgcatctgcc
     3061 ggtgctgcgt ccatttatcc agcgcagcgg agcggtgctt tcggtcgact ggcgggtatc
     3121 gatcgccgaa ctcggcgacc ttaagatcca gggtaacctg gacccggctg ctctcgaaag
     3181 tgagccaagt gcggttcgcc gtgaaaccgc ccgcatcctg gcagaacgcg cgggtaaacc
     3241 ggggcatgtg ttcaatctgg ggcacggtat ttcccctcag gcgaagctgg aatgcgtcga
     3301 ggcaatggtc gagacggttc aatcatccta gtttaacgat cgctgctgtg gaattccata
     3361 gcagcagaga tttttctccc ggaacctgag aaaaatccgg catttcctgg tatttaatag
     3421 atagaggcac catgaaatgc accaggcaac ctaagtttct tgcgaacaaa cccgtacaga
     3481 tttctgacac ctgggaagtc cgcctgtcac cccgtatctg gaataatatc cgccaactag
     3541 cagcatcgcg ccagagcaca ctgaccgctg taacgcgcta ttgtcttttt agcatgatag
     3601 aagtgccaaa ccttcggatg cgtcgacctc tgaggcaatg cctgcagcaa acaataaacg
     3661 aagcgcggtc gtcgaatacg ctgcaccgac atattatttg cttctatggc gaagatcttg
     3721 tcttagtaaa gctgacagct atgcgtctgg gcgtgaccgt atcggcactg gttcgcattg
     3781 ctttatgtct tttcttacct cgccttgctg cggaaatcca gaacaggcac tacgtcagcg
     3841 aggagcatta ctatactcac agtatcaaac gctggatgag aattgtacct tcagcgctga
     3901 atcttgaccg catacccatg atgagaggtt ttgcctttgc aagctttccg ccctggtttt
     3961 ggtggtaatt gctaagcccg ggccaggtaa gacaccgtct caaaatggtg ggtccaggga
     4021 aagaaatcaa agacctccat ctgttcacag cgataacctg cctcggcaaa ggcagccaga
     4081 tcccgcgcca tcgtggctgc atcacaggat gagtaaatga tcggtcctga aaactccccg
     4141 agggcttcag cacgcaatct cttccaggtt tccggcacga ggcctgcacg cggggggttg
     4201 atcagcagaa tttccggccg gaacgccctg atttcggcaa gcagatcgtc acgaatgcgc
     4261 tccggcagaa aaaggtcaac ttttcgataa cgttcggcaa aatgatcagg cagaggatgc
     4321 ccatgctctt caaagcgtac ctgtcgtacc tggtcagggt taaggatatc cagggccatc
     4381 acctgcatcg ggtggatgag cgactgcatg cgcgaaataa agccgaaacc tgaaaagagt
     4441 tcaagcacac gcgggttgga aatgccggta cgcgcaaaat attgtgcaat ggttgtgagc
     4501 cagtacggca gcgcccgcag gttcgcctgg aagaagcccg ttaccgggaa atgaatactg
     4561 atctctcgtc ctgcaggcca ggccaccgaa tatcgggcga tagactgtga ctcttctttg
     4621 gaattaagga cagaaaagac cacctcaccg tcggtattct gacgaatacg tgccgcgcgc
     4681 acttcgacag ggaaacgggg aaatttctct cgcagtgatt tttcatcaaa aatcaggcaa
     4741 ccgtgttcgg gaaaggcacg tgtccggtaa gagaccggtt catagaaacc gtatgtgccg
     4801 aacttgttga tgtgtacctg cacgtggttc cggtaatgcc agcgggtgtc ttcgggcagg
     4861 ctatggaatt ccatagcagg gacttcgact ttgttcctgt ctagctgtga ctgaagccat
     4921 gcctgcttgt agtataaggt gcgctcatag ctcatgtgct gcatgcggca gccgccgcag
     4981 cgcaggtaat gatcacaagc aggcttccca cggtcctctg caaggttgcg cagcgcccag
     5041 gtctcgtgcg caaagttgcc ctcacccacg aactctgcgg gcagctcgtc aatccgcgca
     5101 agcacaccgt gcgcgacctt cgatttcacc tgggccaggc gagccaagaa acgttcaccc
     5161 ggaagcaccc cccaaatgaa gaatacgtgc ggtttgaact tttccttcag ggactcgtcg
     5221 gcgaaatagg agagcccaag accttcggcg acgaagtgcc tgcactccag ctcgacaaaa
     5281 gcaccgggtc taaatctgcg aatattcatg aaaccagcag actagctatg gaattccata
     5341 gctagagtaa atcaatgccg aaaatgccgc attcccgaga agagtagcgc aatcccgtgc
     5401 tcattcgccg ccgtaatact ttccgcatct ttaatcgaac cacccggctg cacaatcgcg
     5461 cgaaccccaa tcttcgcgca ggtatcgacg ctgtcgcgga acgggaagaa cgcctcagat
     5521 gccatcacag agcccttgag cgacaacttc gcatcggcgg ctttttcctg cgcaatacgt
     5581 atgctgtcga tacggctcat ttgtcccgcg ccgatcgcga ggctgcggcc acccgatgcg
     5641 aaaacgaccg agttggactt cacgaacttt gccacacgcg aggcgaatgc gaggtctttc
     5701 atggtttgat catcgcactg cgccttcgag acgaccttga tgtcggcctc gttccacagc
     5761 ggcaggtcct ggtcctgcaa caggtaaccg ccagcgcccg gcaccgaacg caggtccctg
     5821 gtctttgccg cgtcgagctt accgaaatca acggtaatca ggcggaggtt tttctttgct
     5881 gcgagaattt cgaatgcacc ttcgataaac tcaggtgcga tgatcagctc gaagaagtgc
     5941 ggagcgagtt tttctgccgc tgctttgccg agcgggcggt taaagaccac gataccgccg
     6001 aaaaagctga ccgggtcgca gtccatcgcg tgctgcagaa tctgtgcctg gtcgttgccc
     6061 gtggctacgc cgcaggggtt cgtgtgcttg aagatcgcgc acaccggctc gctgaaatct
     6121 gctgccagac gaatggcaat atcaagatcg agaatattgt tgaatgataa ttctttaccg
     6181 gcgagctgtt tccacggcac atctttgccg gcttcgacga ggtaagaagc attctggtgg
     6241 ggattttccc cgtaacgcag cccctgcttc tcgacaaggc tcaaacgcat tcccgcaacg
     6301 ccgctcttct cttcgaggta agcggaaatc gccgcatcgt acgcagcggt acggcggtag
     6361 gctgccaggc gcagctcttc acgcagcgca agtgatgtgg cgcccttatt cgcctcgagc
     6421 tcagcagcga ctgcggcata ctgttcaggc gaaacgacaa tcgccaccga tgcggcgttc
     6481 ttggctgccg agcgcaccat cgccggcccg ccgatatcga tattttcgac agcctcttca
     6541 aacgtcgtgc cggccttcgc tgtaaccgct tcaaaaggat agaggttaat gaccaccagg
     6601 tcaaagagct tgatatcgtg ctggcgcgcc gcctcgagat gcgattccag gtctcgccgc
     6661 gccagaaatc caccatggat cttgggatgc agggttttca cgcggccatc catcatttca
     6721 gggaaaccgg tgtaatcttc tacagcgaga gcgggcaaac cttgttcgcg caggtacttg
     6781 agcgtaccgc ccgtcgagta gatctcgaca cccagcgagt gcagggtcga tgcaaactct
     6841 ttgagcccgc tcttgtctga aacagaaatg agagcccggc gaattggtac taaattgttc
     6901 atggtgcgaa agtatatccc cgggcaatgc ccggggccaa aagggaaatc aaatcaacat
     6961 ggtctggcct tcaaagacgt ctttgcaggg gccagtcatg tatacgtggt tgtctttttc
     7021 agaccagtag atctcgagaa taccaccgcg cagttcaacc tttgccttgc gcttgatctt
     7081 gccagagaga acaccggcaa caaccgctgc gcaggcaccg gtacctgaag cgagtgtctc
     7141 accgacaccg cgctcccaga cacgctgctt gatgtggtct tcgtcaacca tctggatgaa
     7201 gtggacgttg gtgcggttcg ggaacagaga gtagttctca atcatcgggc cgtacttttg
     7261 caccgggaaa ttgtcgacat cgtcgaccca gatcaccacg tgcggattac ccatcgaaag
     7321 cgccatgaac tcgaaagtat tatggtcttc gaggtagatt ttttctttga ggactttctt
     7381 ctcttcttcg ccggctgcgc agaccatggg gatcttcgcg cgctcgagaa tcggctcgcc
     7441 catatcggct ttcacgcggt agacgcggcc gtcgcggacc atcaggatgc aggggataat
     7501 gccgcgcagg gtttgcacgt tgatttcttg tttcttggta ttgccgcggt cgtagaggta
     7561 tttgccgagg cagcggatcg cgttaccgca catctctgct tctgaaccgt cggggttaaa
     7621 aatgcggatc tggaaatctg cttcgttctt gttgtccggt ggcatgatca gcacgaggcc
     7681 gtcagcgccg acaccgtagt ggcggtcaca gagaagccga gctgcacgct gtgggttctt
     7741 gggagaatct tcggtaaaga ggtaatcgtt ccccaaacct tcgagttttg caaaccgtaa
     7801 tgctttagag ccgctcattc aagcctccga tgcggaaaat ctggtaaaaa gtcttgcaaa
     7861 tgcttcaaac tttcgcagaa tgccgggtat tttaacagcc agttcctatg aacgagacag
     7921 agtttcaagc cttatttgag tcctaccaaa aggccttgaa aagtggctct caaaatccga
     7981 ttccacaaaa actccacctg ctcgtctaca actat
//
LOCUS       DAOQJF010000130         8008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4884_length_8008_cov_6.339872, whole genome shotgun sequence.
ACCESSION   DAOQJF010000130 DAOQJF010000000
VERSION     DAOQJF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_8008_cov_6.339872"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLF85_08265"
     CDS             complement(<1..328)
                     /locus_tag="PLF85_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19698.1"
                     /translation="MLSLNTLNENSASLRPQEPKARPRQAEERNQQADFQSYLVSALQ
                     QQPRAELPREQVTPRQEQAVAERPPSHAAQTLRAESPADNQVAPPAQKAGHEAPRDGE
                     TTANTTD"
     gene            complement(533..1483)
                     /locus_tag="PLF85_08270"
     CDS             complement(533..1483)
                     /locus_tag="PLF85_08270"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE19699.1"
                     /translation="MRTETKQATVPENAQGQRLDAWLAARYPVLSRNEWQQRIDGGHV
                     TLNARIARASRRLNSGDNIEFSYTMRDEPEVPTEIGIVYEDDDYLVVNKPPGLPVHPS
                     GIYKTQTVTTLLVTRNILTEPYLLHRLDRETSGVLVLAKNRRAAALFQKILRQGHTEK
                     TYLVAVEGNFTTPMDAAGYIFRLPASRLPRQRFFSETPPPANALEVQHCRTLLKPVEE
                     RAGISLVEATLLTGRMHQIRATMHSLGYPVIGDKLYGVDPALYFTFADEEMKATDWQR
                     LRMPRSALHCRRIRLRHPLTGVDWVLEAPLPDDMGSLFRH"
     gene            1538..2263
                     /locus_tag="PLF85_08275"
     CDS             1538..2263
                     /locus_tag="PLF85_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE19700.1"
                     /translation="MRKTQIIFSSLLTLVLSAAACKKAAEGAASGTVPASTTVTSPDL
                     KMRIAPNTTAVEIARLTRGEQVKIVQRSVEPVQIGKFNAHWYKVTNSSGLTGWVYGAH
                     LAVESDEGDAKAAAEQAEKKLRTAMLGRWEAAKITGALTANFVTLMQDGQIEFGSNRK
                     ATQYGRYTIAMADGYAVVQVTEVPKPMMTDLKAKMVGETLVFTAMMNGTEYKLNLAEK
                     DPELFKEQDKKKAQEAAAGAPQQ"
     gene            2413..3069
                     /locus_tag="PLF85_08280"
     CDS             2413..3069
                     /locus_tag="PLF85_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE19701.1"
                     /translation="MTELNSYIDEDKIAETVLLVDGERIFVKTDTAVVLLNKPKGVVC
                     SHRKQAIRGKHLQTVFDLVPKEYATWFFAGRLDVSSEGLVVLSNDGDHIYALTHPAGG
                     ALKKYYVRTSRPLSEAERERTMKGIIDKGEKLRFVQIEPLALPAEYHVWLREGRNREI
                     RRLMERVGVHVRRLVRLELGPYTLGELSSGKWVLHEKGLVQKQVVSHLTHTTRKHKKT
                     "
     gene            3069..4058
                     /locus_tag="PLF85_08285"
     CDS             3069..4058
                     /locus_tag="PLF85_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNE19702.1"
                     /translation="MAEITIREALNIALAEELARDKNVFLIGEEVGHYNGAYKVSQGL
                     LHKFGEDRVIDTPIAEAGFAGLAIGAAMAGLRPIVEFMTWNFSLVAIDQVINNAAKMH
                     LMSGGQFRVPIVFRAPQGAGGSLSAQHSSVLENFYTYTPGLHVVAPATAADMRGLLKT
                     AVRSDDPVIFLEHEKIYAMKGELAEPENSDYVVPFGKARLVESAEPAQLTILCWSMAY
                     YHVMQAVERLRAENIHADVLDLRSLKPVDSEAIIAAAAKTHRLLIVQEATPVASYGSW
                     LSHHIQDIAFDELDAPVAVVSSDDVPMPYAFSLEPQVLPNGDRIVAAAKSLVA"
     gene            complement(4096..4650)
                     /gene="def"
                     /locus_tag="PLF85_08290"
     CDS             complement(4096..4650)
                     /gene="def"
                     /locus_tag="PLF85_08290"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNE19703.1"
                     /translation="MAVKPVLTIQDERLYKVSEAVTKFDKGLRDLVRDMFDSMAAEGG
                     VGLAAVQIGILKRVLVIDLVEKGFIKGAFINPRLLEASKEMQDGEEGCLSVPGLSAEL
                     KRPKWVKIGYQDMSGNEKVVEGEMLLARAFLHEMDHLDGKVFVDQLEPATHKMLAGDV
                     DRIKRGLPAEHPEEPRYRNRKKSA"
     gene            complement(4783..5664)
                     /gene="sucD"
                     /locus_tag="PLF85_08295"
     CDS             complement(4783..5664)
                     /gene="sucD"
                     /locus_tag="PLF85_08295"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNE19704.1"
                     /translation="MAVLITEKTRIIVQGITGKEGSFHTGQMLEYNTKAKVVGGVTPG
                     KGGQKSEHGLPIFNSVKDAVDKEAANASVIFVPPAFAADAILEGITAGLDLVICITEG
                     IPVRDMSRIVDYIRNSKTVVVGPNCPGVITPGVGKMGIMPGFIHTPGTIGVVSRSGTL
                     TYESVHQLSKVGLGQSTCIGIGGDPIIGLPHIKAVKLLAEDPGTEGIVMIGEIGGSAE
                     EEAAEYIKAHVKKPVVGFIAGQTAPPGRRMGHAGAIISGSAGTAQTKMKAMKDAGIFV
                     CESPAEIGETMKKALGK"
     gene            6284..6517
                     /gene="rpsU"
                     /locus_tag="PLF85_08300"
     CDS             6284..6517
                     /gene="rpsU"
                     /locus_tag="PLF85_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNE19705.1"
                     /translation="MQKLADISQHFSNGIEVRDNEPVEGAIKKFKREVANSGILTELK
                     KREYFEKPSVIKKRERDQAERKRFRKRNIYSEA"
     gene            6581..>8008
                     /gene="dnaG"
                     /locus_tag="PLF85_08305"
     CDS             6581..>8008
                     /gene="dnaG"
                     /locus_tag="PLF85_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNE19706.1"
                     /translation="MATDADRILAAIPIENYIGRFIALKRKGNNLWGLCPFHGEKTPS
                     FSVSPQKGIYKCFGCGVGGNVITFAKEYNKLPFPEALKLLAEFAGIELSHQPRRPGED
                     DRKRALLELHLWAHKLYQTQFAGSDAERYSSSRGITPRAVTAFELGYAPDQPRYLETR
                     LNERYRNEPQLLAKAIENLGTIGLTGRNDSDTYNRFRDRLIFPIKDLRGQIIAFGGRL
                     VREKENAGKYINSPETPLFNKSNSVFRLGESAQAIRKEGFVIICEGYLDCLGLFEAGL
                     ENAVAPLGTAFTGEQAKQIKRFTDNITLFLDNDPAGTEAAYKALRVARKANLAIRIVH
                     PLSQGDKQDPFDLSRKLSTEEMRFLVSQAHSEVKFLIWYTFAFKYNTSDLSQKKKALA
                     DFYEYVRILETELERDEFLRAAASALAVDAQILRKDFSAPTQAGKPAGGFIAAEAKAR
                     EQASQPQKPKPVSKQEKEVIAMLLRF"
BASE COUNT         1835 a         2140 c         2212 g         1821 t
ORIGIN      
        1 tgtctgtagt gttggctgtg gtttcgccgt cgcgaggtgc ctcgtgcccg gctttctgtg
       61 ctggtggtgc aacctggtta tcggccgggc tttctgctct caaggtttgt gctgcatggc
      121 tcggggggcg ttcggcgacc gcctgttcct ggcggggagt tacctgctca cggggaagct
      181 ccgcccgggg ttgttgttgc agcgcggaga cgaggtagga ctggaaatct gcctgctggt
      241 tgcgctcttc ggcctgccgt gggcgtgcct tgggctcctg cgggcgcagc gatgcagaat
      301 tctcgttgag cgtattcagg gaaagcatct gtattccaat atatcgggtg ccgctcgaaa
      361 tgctgtagcg gaatttgaga gcacgacgcg cgcaccggta aaattgttct aaagcttttg
      421 ccgtgagccc tgatgtggca taataaagca cgctgatgtg cagataatgc gtgtatcaaa
      481 aagtgacata aaccggcagg gaatccacag cgttttccac agatgtggat aactaatgcc
      541 ggaaaaggct gcccatgtcg tcgggtaggg gggcttcgag tacccagtct acacccgtta
      601 gggggtgcct gaggcgtata cgccggcagt gcagggccga acgcggcatc cgcaggcgtt
      661 gccagtcagt tgctttcatt tcttcgtcgg caaaggtgaa atagagggcg ggatcaacgc
      721 cgtagagttt gtcgccgatc acggggtatc ccaacgaatg catggtggca cgtatctggt
      781 gcatgcggcc cgtgagcagc gttgcctcca ccaggcttat acctgcgcgc tcttccacgg
      841 gtttcagtag ggttcggcag tgctgcactt cgagagcatt ggccggcggc ggggtttcgg
      901 aaaaaaaacg ctgccttggc agccggctgg caggcaggcg gaagatatac cctgcagcat
      961 ccataggtgt cgtgaaattg ccctccacgg ctaccagata ggtcttctct gtgtggccct
     1021 gccgaagaat cttctggaac agggcagcag cccgccggtt cttcgccagc accagcacac
     1081 cactggtctc ccggtcgagc cggtggagca ggtacggttc ggtgagtata ttgcgcgtga
     1141 cgagtagtgt ggtgaccgtc tgcgtcttgt atatacccga agggtgtacg ggcaggccag
     1201 gtggcttatt cactaccaga tagtcatcgt cttcgtagac tataccgatt tctgtgggca
     1261 cctccggttc atcgcgcatc gtgtaagaaa attcaatatt gtcaccagag ttgagccggc
     1321 gcgatgcccg ggcaatgcgt gcgtttaatg tcacatgccc gccgtcgata cgttgctgcc
     1381 attcattacg cgacagaacc ggataacgcg cggcgagcca cgcatcgagg cgctgtccct
     1441 gtgcattctc gggcactgtt gcttgttttg tctctgtgcg catggttcgc ggtgcccaaa
     1501 tgtgttgacc cggccggtcg taattgaatg cgcagctatg cgaaaaacgc aaattatctt
     1561 ctcttcactt cttacgctgg tcctttcagc agctgcctgc aaaaaagcag cagagggagc
     1621 ggcctcgggg actgtacctg ccagcacgac ggtgacatca cccgatctca agatgcgcat
     1681 tgcgccaaac acgacggcag tcgaaattgc gcggttaacg cgcggtgagc aggtcaaaat
     1741 tgttcagcgc agcgttgaac ccgtgcagat cggcaagttc aatgcccatt ggtacaaagt
     1801 cacgaatagc tcaggtctta cgggttgggt ctacggagca caccttgcgg tggagtcaga
     1861 tgaaggcgac gcaaaggctg cagctgaaca ggcagagaaa aaactccgca ctgcaatgct
     1921 tggccgttgg gaagctgcga agattactgg tgcgttgacg gccaactttg tcaccctcat
     1981 gcaggacggc cagatcgagt ttggctctaa tcgtaaggcg acgcaatacg gcaggtatac
     2041 catcgccatg gccgacggtt atgcagttgt ccaggtgaca gaagttccaa aacccatgat
     2101 gaccgacctc aaggcaaaaa tggttgggga gacgctcgtt tttaccgcga tgatgaacgg
     2161 tacggagtat aaacttaacc ttgcggaaaa agatcccgag cttttcaaag aacaggacaa
     2221 aaagaaagca caggaagctg ccgcgggcgc tccgcagcaa tagccgggta atttctgcca
     2281 aaacaagaaa cacacctctc ggacagcaag aagttacgca tcggcaaatt gctggcgcgt
     2341 gccggcttat gcccgcgcag agctgcagca gcatttcttg ccgaacatga cgtccgcctg
     2401 aatggcgaac gtgtgactga actcaatagc tacatcgacg aagacaagat tgccgaaacc
     2461 gtgctgctcg tcgacggtga gcgcatcttc gtaaagaccg ataccgcagt ggtgctcctg
     2521 aacaaaccca aaggagtagt ctgctctcac cgcaagcagg ccatcagggg aaaacacctg
     2581 cagactgtgt ttgatcttgt gcccaaagag tatgcaacct ggttctttgc gggccgtctt
     2641 gatgtaagct cagagggtct tgtggtgctt tcgaatgacg gcgatcatat ctatgcgctc
     2701 actcatcccg ccggtggtgc tctgaaaaaa tactacgtgc gcacgagcag accgctctcc
     2761 gaggccgaac gcgagcggac catgaagggc attattgaca aaggggaaaa actccgtttt
     2821 gtccagatcg aaccgctcgc tttgcctgca gaataccatg tctggttacg cgaaggccgt
     2881 aaccgcgaaa ttcgccgcct catggagcgg gtgggggtac acgtccggcg acttgttcgc
     2941 ctcgagctcg ggccatatac tttgggtgaa ctatcatcgg gcaaatgggt cttgcacgaa
     3001 aaaggcctcg tacagaaaca ggttgtttca cacttgacgc acactacccg caaacataaa
     3061 aaaacctgat ggcagaaatt accatccgcg aagcactcaa tatcgcactg gccgaagagc
     3121 tcgcgcgcga taagaatgtg ttcctcatcg gcgaagaagt cgggcactac aacggcgcct
     3181 acaaagtatc ccagggtctt ctgcacaaat ttggcgaaga ccgcgtgatt gatacaccca
     3241 ttgctgaagc gggctttgct ggccttgcga ttggcgcggc gatggcgggc ctgcgtccga
     3301 tcgtcgaatt catgacctgg aacttttcgc tcgtggcgat tgaccaggtc atcaacaacg
     3361 ctgccaagat gcacctgatg tccggcggcc agtttcgcgt tcccatagtc tttcgcgctc
     3421 cgcagggggc cggcggtagc ctgagtgcgc agcattcgtc agtgctcgaa aacttctaca
     3481 cctatacgcc aggtttgcat gtcgtggcac cggccacagc ggcggacatg cgcggcctgc
     3541 tgaagactgc agtccgcagc gatgatccgg tcatattcct ggaacacgaa aaaatctacg
     3601 ccatgaaggg tgaactcgca gaacccgaaa actccgatta cgtggtacct tttggcaagg
     3661 cgcgccttgt cgagagcgca gaacctgcgc aactgactat actctgttgg tctatggctt
     3721 attaccatgt tatgcaggca gtggagcggc tgcgcgcgga gaatatccac gccgacgtgc
     3781 tggacctgcg ttccctgaag ccagtcgact cagaggccat aattgcagca gcagcgaaaa
     3841 cgcatcgcct gttgattgtg caggaggcaa cacctgttgc ttcgtacggt tcatggctgt
     3901 cacaccacat acaggatatt gctttcgatg agctggatgc cccggtggcg gtagtttctt
     3961 cggatgacgt gccgatgccc tatgcgttct cgctcgagcc gcaggtcttg ccgaatggag
     4021 accgaatcgt tgccgctgct aagtcactgg ttgcctaatt tccttaatta aggaaatcgg
     4081 gcttagctcg gcctgttaag ctgatttctt gcggttacgg tagcggggtt cttcggggtg
     4141 ctcagcgggt aatcccctct tgatgcggtc gacatcgccc gcaagcatct tgtgtgtagc
     4201 gggttcgagc tgatcgacaa ataccttacc atcgagatgg tccatttcat gtaaaaatgc
     4261 ccgcgccagc agcatttcac cttcgacgac tttctcgttg ccactcatgt cctggtaacc
     4321 aatctttacc cactttggtc gctttaattc ggctgaaagg cccggcactg aaaggcagcc
     4381 ttcttccccg tcctgcattt ccttcgaagc ttcgagcagc cgcgggttga tgaaagctcc
     4441 cttaatgaaa cccttctcga ccaggtcgat cactagcacc cgctttagga tgccgatctg
     4501 tacggcggca agcccaacgc caccctcggc tgccatggag tcgaacatgt cgcggacgag
     4561 gtcgcgcaat cccttatcga atttcgtaac ggcctcggag actttgtaca gacgttcatc
     4621 ctggatggtt aatacgggtt ttactgccac gggaaaaatc taagccgaaa atcgagacag
     4681 ggcgaaaata tcttctgtgg aaacaggggg agtttccgta attaaggaaa aggtcaaaaa
     4741 aaagccggca aagccggctt ttgggaaggc gatttgggca gcttatttgc ccagtgcctt
     4801 cttcatggtt tcaccgatct ccgcaggcga ttcgcaaacg aaaatacccg catctttcat
     4861 cgctttcatt ttcgtttggg ccgtacctgc tgaacctgaa atgatggcac ccgcatgccc
     4921 catgcggcgt cctggagggg ctgtctggcc agcgatgaag ccaactacgg gctttttcac
     4981 gtgtgccttg atgtattccg ctgcctcttc ttcggcgctg ccgccgattt caccgatcat
     5041 gacgatgcct tcagtacccg ggtcttcagc gagcagtttc accgctttga tgtgcggcag
     5101 gccgatgatg ggatctcccc cgataccaat gcaggtactc tggccaaggc ctactttgct
     5161 caactggtgt accgattcgt acgtcagcgt accagagcgc gatacgaccc cgatggtgcc
     5221 gggcgtgtgg ataaagccgg gcatgatacc cattttgcct acaccgggag taataacgcc
     5281 cgggcagtta gggccaacca caactgtttt gctgttgcgg atatagtcga caatgcgcga
     5341 catatcgcgt acgggaatgc cctcggtgat acagatcacg agatccagac cggcggtaat
     5401 tccctcgaga attgcgtcag ccgcaaaagc cggcgggaca aatattacag aggcgttcgc
     5461 ggcttcttta tctacggcgt ccttaacgct gttgaaaatg ggaaggccgt gttcagattt
     5521 ctggccacct ttacccgggg taactccccc gactactttt gctttcgtgt tgtattcaag
     5581 catctggccg gtgtggaatg acccttcttt gccggtaatg ccctgaacga tgatgcgcgt
     5641 cttttcagta atgagaactg ccatagagtt tgagttcaaa cggaaaagag ggcgtcaagg
     5701 agttagaggg ggtgggattg taaagaagcg cagctcctcc ctgaatgcca tcaggccatc
     5761 ggtccacccg attacttgga aatcataatt aaggacaccc tgttcagacg ctgttcgctt
     5821 ctgtaacttt tccaaaagat gagagattaa tagagacacg ctaattctgg ttgcagtggc
     5881 aaaactgtgc aggcgattgt acgtggacaa gggccaatac acactgatga tagcgcctcc
     5941 ttcaggacat tgttggtgct gtcgcaggcg ggaacgtgtc ggtgtgaacc gtcgtgcgct
     6001 acgtatgagg gctggtagtc tgcttagcag ggcacggtgc ttgcggttcc aggtggagag
     6061 cagtcgtttt ggcagataga tgctggtttt cttcatgcgc ctctactatt taaataccgg
     6121 caaacggcag aattttctca cggaatttga aaaaaataat gagacataat aaactatctc
     6181 ttgcttaatt aaggaaaccc tcaacggcta ccgcaaccgc tgatttccct gaatcattga
     6241 cgccctgtct tgaaccccca cgttaaccgc tgacggaggc ttagtgcaaa aactagcaga
     6301 catttcacag catttcagca atggtatcga agttcgtgat aacgaaccag tagaaggcgc
     6361 gattaagaaa ttcaagcgcg aagtagcaaa ctcaggtata ctgaccgagc tcaagaagcg
     6421 tgagtacttc gaaaagccaa gcgttatcaa aaaacgcgaa cgcgatcagg ccgaacgcaa
     6481 acgtttccgc aaaagaaaca tctacagcga agcttaatta gttcgtacgc cgcaggcgct
     6541 ggttgacacg ccgtccgcgt tcggcgtgct tccttaatac atggcaacag acgccgaccg
     6601 cattcttgct gccattccca ttgaaaatta catcgggcgc ttcatcgccc ttaaacgcaa
     6661 aggcaataac ctctgggggc tctgtccgtt tcatggcgag aagacccctt cgttctctgt
     6721 atctccacaa aagggtatct acaaatgttt tggctgtggc gtgggcggca atgtcattac
     6781 cttcgccaaa gaatacaaca agctcccatt tcccgaggcg ttgaaattac tcgccgaatt
     6841 cgcggggatt gaactctctc accaaccacg gcgcccgggt gaagatgacc gcaagcgtgc
     6901 gttactcgag cttcacctct gggcgcacaa gctctaccag acgcagttcg caggctccga
     6961 cgctgaaaga tacagcagca gcagggggat tactccccgt gctgtaacgg ccttcgagct
     7021 cggttacgct cccgaccagc cgcgctatct cgagacgcgg ttgaatgaac gttaccgcaa
     7081 cgagccacag ttactcgcca aagcgattga gaacctgggg acgattggcc ttaccggtcg
     7141 caacgacagc gatacttata accgctttcg cgaccgcctg attttcccga tcaaagatct
     7201 gcgcgggcag atcattgctt ttggcggccg gcttgtgcgt gaaaaagaga atgcgggcaa
     7261 gtatatcaat tcacccgaga ctcccctttt taataaatcg aactcggtgt ttcggctcgg
     7321 cgaatccgca caggccatcc gcaaagaggg atttgttatt atttgtgaag gttacctcga
     7381 ctgccttggc ttgtttgaag cgggactcga aaatgccgtt gctcctttgg gtactgcatt
     7441 caccggcgag caggcgaaac agatcaagcg cttcaccgac aacatcacgc tctttctcga
     7501 taacgacccc gcgggcactg aagctgcgta caaggcgctg cgggtggcgc gcaaagcgaa
     7561 cctggcgatt cgtattgtgc acccgctctc gcagggtgac aagcaggacc cgtttgatct
     7621 cagccgcaaa ctttctacag aagaaatgcg gtttctcgtg tcacaggcgc acagcgaagt
     7681 gaagtttctg atttggtaca ctttcgcctt caaatacaat acttctgacc tgtcgcagaa
     7741 gaaaaaggcg ctggccgatt tctatgagta cgttcgcatc ctggagacgg agctcgaacg
     7801 cgacgagttt ttgcgggctg cagcttctgc tctcgcggtc gatgcgcaga tcctgcgcaa
     7861 agatttttcc gcacccacgc aggcaggcaa acctgccggt ggatttatcg ccgcagaggc
     7921 caaggcacgc gagcaggcct cgcagccaca aaaacccaaa cctgtcagta agcaggaaaa
     7981 ggaggtcatc gcgatgctgc tccgtttt
//
LOCUS       DAOQJF010000131         7974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4916_length_7974_cov_4.616745, whole genome shotgun sequence.
ACCESSION   DAOQJF010000131 DAOQJF010000000
VERSION     DAOQJF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4916_length_7974_cov_4.616745"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1120
                     /locus_tag="PLF85_08310"
     CDS             149..1120
                     /locus_tag="PLF85_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNE19707.1"
                     /translation="MFGWAQNFADWVVYSIFAVAPGHFAASLDFFIFDTIKLFVLLGG
                     ITLLMGTVNSYLPVDAVRDFLTKRRLYGLDYLLAALFGVITPFCSCSSVPIFIGFVKG
                     GIPLGITFTYLITAPLVNEVALAVFAGSFGMRVTGIYVATGMVMGIAGGYLLGRLQLE
                     RYLSPWIQQMIGGGNGAEKLAADERPFSARLSGIAREALHTIRGIAPYIVLGIGIGAT
                     MHGFLPTGFFEAYIRKDQWFAVPLAVGMGVPMYSNAAGVLPVVEVLVAKGIPLGTAIA
                     FMMGVIGLSLPEALLLKKIMTWRLVGIYFGIIALLMMVSGFLFNAWL"
     gene            1164..1499
                     /locus_tag="PLF85_08315"
     CDS             1164..1499
                     /locus_tag="PLF85_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE19708.1"
                     /translation="MISKSVEFSRDEQQIASMAKALAHPARIAILKLLAAKQDCMCGD
                     IVEELPLAQSTVSQHLKQLKDAGLIKGEIDGPKVCYCIDYKALAKFKADLDSVFSEID
                     ERREASECC"
     gene            1490..2665
                     /locus_tag="PLF85_08320"
     CDS             1490..2665
                     /locus_tag="PLF85_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19709.1"
                     /translation="MLLNADYLNEHCHCSTLDKSKIEHAHFFSQHPHFLSLDDLHQMQ
                     AFIEAHERLLGVGLPALSPAEGNLPLKAVERGIFNSYDFHISDNGPQLIEINTNAAGA
                     LLGLHAEKEMMQCCEGLPGYLSHPGFAFDSGKIVEMFRREFALRFPGRSLRRVAIVDE
                     NPREQFFYSEFVLVQQTLQSHGIDAVIVAPEALTIRENVRYANELPLDFIYNRLTDFA
                     FEKPSSATLKQIYEHGLATVSPNPAVHALYANKANLTLFWSDAANNMPQTDLATVRAH
                     LPETVFVTAANADLLWSQKKEWFFKPVDGYGGKGVYRGDKLTTKVWQHIIGGSYIAQR
                     YIAPRTRQVTEATSLKYDVRVYTYAGEILGLMARYYQGQTTNFRTEHGGLAGVLVMR"
     gene            complement(2669..2953)
                     /locus_tag="PLF85_08325"
     CDS             complement(2669..2953)
                     /locus_tag="PLF85_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19710.1"
                     /translation="MEMLFIVLVYFSNGNFQSLEVIEMSRAEAELGPKKNSNASCEVA
                     LAKPLAALRNKSVKKMAQRKIHYACLGASQVGTLESLTNQSQSWPAVTGK"
     gene            3086..3418
                     /locus_tag="PLF85_08330"
     CDS             3086..3418
                     /locus_tag="PLF85_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE19711.1"
                     /translation="MIEVTRHGNAVVFRVKETQLQMYAIPQFKATVTSQLIDKPKLVV
                     FDMTLVEHVDSSAMGALFHFQKELKTWGGELRVAHLTSKVMQIFKITKSENAFEICDT
                     VAEALKGA"
     gene            complement(3424..4899)
                     /locus_tag="PLF85_08335"
     CDS             complement(3424..4899)
                     /locus_tag="PLF85_08335"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE19712.1"
                     /translation="METQHFDDAQRKKFDVLIIGAGISGIGAACHLKRELPHKSFAIL
                     ERRAAVGGTWDLFRYPGIRSDSDMNTFGFGFRPWTETRVLSDGPSIKKYVEDTAREYD
                     VFRHIRFGTKVTSARFSSSDARWTIESVNEKNGEKSVYTANFIIACTGYYNYDQGYQP
                     EFPGEKTFKGQIIHPQKWPENLDYSGKKVVVIGSGATAVTLIPAMAPKTAHITMLQRS
                     PTYIVSIPSNDFLSETLRKFLPKSWVYQMARLRNVALQRLIFMLAKARPGAIRRLILA
                     GAKRQLGDAVDIKHFSPSYNPWDERLCVVPDGDLFETLKSGRASIVTDQIETFTEKGI
                     KLKSGKELEADIIVTATGLNVELMGGVEVEVDGAKVPVPERVTYKAVLVEGVPNAAII
                     FGYTNASWTLKVDIACEYICRLLKHMDKNRLHTVVAHAPADIRTEDTVMGGLSSGYIR
                     RAANKLPRQGKTGVWKVTNDYLSDSAMLKLTPVTDDALKFS"
     gene            5069..5809
                     /locus_tag="PLF85_08340"
     CDS             5069..5809
                     /locus_tag="PLF85_08340"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNE19713.1"
                     /translation="MAQLKVDRRTAIFMLTLSGIPAMWFSLLFLNDCLLLYRAQAWQQ
                     VDAVVQAAEKGSLPEDDGEAHTSRDLKESLAYGYAFNGNEIVAHRYGPFQTWNVGSNL
                     RGLSTGSSITAYVNPDKPHEAVIDRTLRSGFGIVLALTVLAYLWLAWALLAIFRDERF
                     TRGFMGQLMVFVSALVFVAALLASGDERDTNWWLVAGGFLGGFTLGGFSVIMGLWSQK
                     TSAALALIAILFGITTGIIGIVRGGVRG"
     gene            complement(5830..6216)
                     /locus_tag="PLF85_08345"
     CDS             complement(5830..6216)
                     /locus_tag="PLF85_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19714.1"
                     /translation="MKETSRDFADSHRIEQILKRGANLNEAMPDGNPLVIHLFMNMLK
                     HTPENVRVRQMLIRHGADPNARIVKGFMKGLTLLMLADAATAELLLNKGADPFLKDGS
                     GDTALDHARAQKDQAKIRLLEKAMKK"
     gene            6392..7213
                     /locus_tag="PLF85_08350"
     CDS             6392..7213
                     /locus_tag="PLF85_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE19715.1"
                     /translation="MQFTGTAKYYLSDELAQIVDMALALEKPLLLTGEPGTGKTQLAF
                     AIAESLGLGMEILRAKSTFRGEEAAYTQDTVLRLNDSRFPDAASGRNVQNFYDYIIWG
                     PIGRAFRADQRKVLLLDEIDKTESDVQDNLLDVLEDTEFTIRETNTVVKAKHKPVIII
                     TSNAKRELSDPFLRRCFCHHISFPTIDEMRHIVALHFPDFGGPVLEAALKIFYELRAR
                     GYEKAPATSELIAWIEAIRRKGLTPEEVERTPLPGILLKRAADLLSFRGEKRVRG"
     gene            7213..>7974
                     /locus_tag="PLF85_08355"
     CDS             7213..>7974
                     /locus_tag="PLF85_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19716.1"
                     /translation="MFLNFFYALRDEGLPIGADQVLEFYRAPEKVPVENTDDLFLLLR
                     LICVKKREDLDTFERVFLNHFYNMDIPRVAEGDYALLETRQFREWLREARERGEIPFR
                     QHELNLDELMKKFWETLREQMKEHHGGSKWVGTGGNSPFGHSGHSAGGVRVFGQGRNF
                     SAMKVIGEKRHVSYSSRQSLSADNVRQALALLKNMQKHAAATDLDIEESVYLSEKSGD
                     IELVFPPPLRNQLKVVLFIDNGGHSMLQHVPLTRLI"
BASE COUNT         1692 a         2214 c         2264 g         1804 t
ORIGIN      
        1 gaagtagaaa agatcgaaga tgtcgagcag atcatgcagt acaacgtgct cgcaacaccg
       61 gggttagtga tcgacgaaaa acttcgcata tcgggccgcg tgccaacagt ccaggagatc
      121 aaagacctgg tcaacaaggc gtaacacagt gtttggctgg gcgcagaatt ttgccgactg
      181 ggttgtttat agcatctttg ctgtggcccc ggggcatttt gcggcatcgc tcgacttttt
      241 tatttttgat acgatcaaac tgttcgtgct gctcggcggc atcaccctgc tgatgggtac
      301 ggtgaattcg tatttgccag tcgatgctgt gcgggacttt ctgacgaagc gtcgcctcta
      361 cggtctcgac tacctgctcg ctgcgctctt tggcgttatt accccgttct gttcatgctc
      421 gtcagtaccg attttcatcg gcttcgtcaa gggcggcatt ccgctgggca taacgttcac
      481 ctacctcatc accgctcccc tcgtgaacga agtggcgctc gcggtctttg ccggcagctt
      541 cggtatgcgc gtgaccggaa tctatgtcgc aaccgggatg gtaatgggca ttgccggtgg
      601 ttacctgctc ggccgcctgc aactcgaacg ctatctctct ccgtggatcc agcaaatgat
      661 cgggggcggc aacggggcag agaaacttgc cgcagacgaa cgtccgttct cagcgcggct
      721 gtccgggata gcgcgcgagg ccttgcacac gataaggggc attgctcctt acatcgtgct
      781 cggcatcggt atcggcgcaa caatgcacgg cttcttgcca acgggctttt tcgaggccta
      841 catccgcaaa gaccagtggt ttgccgtgcc tttggcggtg ggcatgggcg taccgatgta
      901 cagcaacgcg gccggtgtgc tgcctgtggt cgaggtgctg gttgcaaagg gtattccact
      961 cggcaccgcg attgcgttca tgatgggagt tatcggcctg tcactgcccg aggcgctgct
     1021 gctgaagaag atcatgacct ggcggctggt cgggatctac tttgggataa tcgcactgct
     1081 catgatggtc tcgggctttc tttttaatgc atggctatga agatattgtg gtcgtttaat
     1141 cgtaaaaata cgataatgaa attatgatca gcaagagcgt cgaattcagc cgcgatgagc
     1201 agcaaatcgc ctcgatggca aaggctcttg cacaccccgc gcgtattgcg attctaaagt
     1261 tgctggcagc gaagcaagat tgtatgtgcg gcgatatcgt cgaagagctg ccgcttgcac
     1321 agtcaacggt ttcgcagcac ctcaagcagc tgaaagatgc gggtctcatc aaaggtgaga
     1381 tcgacggccc caaggtctgc tactgcatcg attataaggc gctggcgaaa ttcaaagctg
     1441 atctcgattc agtattttct gaaatcgacg aaagacgcga ggcgagcgaa tgttgctgaa
     1501 cgccgattac ctcaatgaac attgccactg ctcgaccctt gacaagagca agatcgaaca
     1561 cgcgcacttt ttcagtcagc atccgcactt tttgtcactg gatgacctgc accagatgca
     1621 ggcctttatc gaggcgcatg aacggctttt aggggtcggt ttacctgccc tgagcccagc
     1681 cgaagggaac ctacccctaa aagccgtcga acgtggcatc ttcaattcat acgattttca
     1741 tatctccgat aacggcccgc aactcattga aattaataca aacgctgctg gtgcattgct
     1801 ggggctccat gctgagaagg agatgatgca gtgctgtgaa ggcctgcccg gctacttgtc
     1861 gcaccccggc tttgctttcg acagcgggaa gattgttgag atgttccgcc gcgaatttgc
     1921 gctgcgtttt cccggtcggt cgctcaggcg ggtcgcaatc gtagacgaaa acccacgtga
     1981 gcaattcttc tattcggagt tcgtgctcgt gcagcagacg ctgcagagcc acggtatcga
     2041 cgcagtcatc gtcgcacccg aagcactcac gattagggaa aatgtgcggt acgcgaatga
     2101 gctccccctt gattttatct acaaccgcct gaccgatttt gcgtttgaga aaccatcgtc
     2161 agcgacattg aaacagattt atgaacacgg tctcgcgacc gtgagcccca acccggcggt
     2221 gcatgcgctg tacgctaaca aggcgaacct gacgctcttt tggtcagacg ctgcgaacaa
     2281 catgccgcag accgacctgg cgacagtgcg cgcgcacctg cctgagacag tttttgttac
     2341 agcggcgaac gccgatctgt tgtggtcgca gaagaaagag tggtttttca agccggttga
     2401 cggttacggc ggcaagggtg tctaccgcgg cgacaagctg accacgaagg tctggcagca
     2461 catcattggc gggagctata ttgcgcagcg atatattgca ccgcgcacac ggcaggtgac
     2521 tgaagcgacg agcctgaagt atgacgtacg cgtctacacg tacgctggcg aaattttggg
     2581 gctcatggcg cgctactacc aggggcagac gacaaacttt cgcaccgaac acggcggtct
     2641 cgccggcgtg ctcgtcatgc gataagattc atttgccggt gactgccggc cagctttgcg
     2701 attggttcgt gagagactcg agcgtgccta cctggctcgc accgagacat gcatagtgga
     2761 tcttgcgctg cgccatcttc ttgactgact tattgcgcag ggctgcgaga ggcttcgcca
     2821 gggcaacttc gcaggatgca ttgctgtttt tcttcgggcc cagttcggcc tctgcgcgtg
     2881 acatttcgat cacttcgagc gattgaaaat ttccgtttga gaaatagaca agaacgataa
     2941 atagcatttc catgcggcag accagctggc ttcagtcagg cgttcaaggg gtattttttt
     3001 cgaggagtat acacccgagg cactcaaaaa ggttcacagc ccgtttactg ttgacattct
     3061 acagcgggcc gggaacagtc gacgcatgat cgaagtcacg cggcacggca atgcggtggt
     3121 tttccgcgtg aaagaaaccc aactgcaaat gtatgcgatt ccgcaattca aggcgacggt
     3181 gacgagccag ctcatcgaca aaccaaaact ggtcgtcttc gacatgacac tcgtcgaaca
     3241 cgtcgattca tcggcgatgg gcgcgctctt ccacttccag aaggagctca agacatgggg
     3301 cggtgagctg cgcgtggccc acctcaccag caaagtgatg cagatcttca agatcaccaa
     3361 atcagaaaat gccttcgaga tctgcgatac agtcgccgag gcactgaagg gagcttagcc
     3421 ccttcagctg aacttcagcg cgtcgtcggt aacgggcgtc aacttcagca tcgcgctgtc
     3481 tgacaggtaa tcattcgtca ccttccatac gccggtctta ccctgccgcg gaagtttatt
     3541 cgccgcgcgc cgaatgtagc ctgagctcag accacccatc accgtatctt cggttctgat
     3601 atctgcggga gcgtgcgcca cgacggtatg caggcggttt ttgtccatgt gcttcagcag
     3661 gcggcaaatg tattcgcagg caatatcaac cttgagggtc caagaggcat tggtgtaacc
     3721 aaagatgatg gcggcgtttg gtacaccttc gaccagcact gccttgtagg tcacgcgctc
     3781 gggcaccggt accttcgcac catcgacttc tacctcgacg ccgcccatga gttcgacgtt
     3841 gaggccagtg gctgtcacga tgatatcggc ttcgagttct ttacccgact tgagcttgat
     3901 gcctttttcg gtgaaggttt cgatttgatc ggtgacaatc gaagcacggc ccgacttcaa
     3961 cgtttcaaac agatctccgt cgggcacgac gcagaggcgt tcatcccacg ggttatagct
     4021 gggagaaaaa tgcttgatgt cgactgcatc gccgagctgc cgcttggcac ctgcgagaat
     4081 caggcggcga attgcacccg gacgggcttt cgcgagcatg aagatcagcc gctgcagggc
     4141 gacgttgcgc aggcgggcca tctggtagac ccatgacttg ggcagaaact tgcgcagagt
     4201 ctctgacaaa aagtcgttcg aaggaatcga aacaatgtac gtcggtgaac gctgcagcat
     4261 cgtgatgtgc gccgtcttcg gcgccatggc aggaatcagc gtaaccgcag tagctccgct
     4321 gccgatcacc acgaccttct tgccggaata atcgaggttt tcaggccatt tctgcggatg
     4381 gataatctgg cccttaaagg ttttttcgcc cggaaattct ggctggtagc cctggtcgta
     4441 gttgtaatag cccgtgcacg cgataatgaa gttcgcggta tagacagatt tttcgccatt
     4501 cttttcgttc accgactcga tcgtccagcg cgcatcgctg ctcgagaagc gcgcgcttgt
     4561 cacttttgtg ccgaagcgga tatggcgaaa aacgtcgtac tcgcgcgcgg tgtcttcgac
     4621 atatttcttg atcgagggcc cgtcagaaag cacgcgggtt tcggtccacg ggcgaaagcc
     4681 gaagccaaag gtattcatgt ccgagtcgga gcgaatgccg gggtagcgga agagatccca
     4741 tgtgccgccg acggccgcac ggcgctcgag tatcgcaaac gacttgtgcg gcagttcgcg
     4801 cttgaggtgg caggcggccc cgattcccga gatgccggca ccgatgatca gtacgtcgaa
     4861 tttcttgcgc tgagcgtcgt cgaagtgctg tgtctccatg ttccctcctg cccggcgcct
     4921 ctcgcggcca ccatgctatg gaattccata gccgggcata atgcggcgca taaaggaaac
     4981 tgaacaattc gtcaagtttt gttgagtcat cgcccgctgt cgcagggctt aatgctcgca
     5041 gaaactgcgc agggcgaaaa aaacgcccat ggcgcaactc aaagtcgacc ggcgcaccgc
     5101 tatcttcatg ctcacgcttt cgggcattcc tgcaatgtgg ttttcgctgt tgttcctcaa
     5161 cgactgcctg ctactctacc gtgcacaggc ctggcaacag gtcgacgccg tcgtccaggc
     5221 agccgagaaa ggttcgttgc ctgaagacga tggcgaggcg catacctcgc gtgacctgaa
     5281 agaatcactt gcctacgggt acgccttcaa cggcaacgaa attgttgcgc accgctacgg
     5341 gccgttccag acgtggaatg tcggttcaaa tctcagggga ttgagtaccg gcagcagtat
     5401 cactgcgtac gtgaatcctg ataagccgca cgaggcagtc atcgaccgca cgctgcgctc
     5461 aggctttggg atcgtgctcg ctctgacggt gctcgcctac ctgtggctcg catgggcgct
     5521 gctcgcaatt tttcgcgatg aaagattcac gcgcggtttt atggggcagc tgatggtatt
     5581 tgtttcggcg ctggtttttg tggctgcgct gttggcttct ggcgatgagc gtgatacaaa
     5641 ctggtggctt gtcgcaggtg gctttctcgg cggttttacg ctgggcggat tttctgtaat
     5701 catgggcctg tggtcgcaga agacctcggc agcgctggca ttaatagcga tactgtttgg
     5761 catcaccacc gggattatcg gcattgtgcg cggcggcgtg cgcggctgac gcattctgaa
     5821 gatacttgac tatttcttca tcgccttctc gagcagccga atttttgcct ggtctttctg
     5881 cgcacgcgca tggtcgagtg ccgtatcgcc cgaaccatct ttcaaaaatg ggtcagcccc
     5941 tttattcagc aagagctccg ccgtcgctgc atcagcgagc atcagcagcg tgaggccctt
     6001 catgaaaccc ttgacgatgc gtgcattggg gtcggcgccg tgccggatca gcatttggcg
     6061 cacccgcaca ttctcggggg tgtgcttgag catgttcatg aagaggtgta tcaccagtgg
     6121 gttaccatcg ggcatcgcct cattgaggtt cgcgccccgc tttagtatct gttcgatacg
     6181 atgactgtcc gcgaaatcgc gggaagtctc tttcataaga tcttcaacgt ttccggcaat
     6241 tgcgggtgag ctcataagaa gtaagaggga gcagatccgg gaagcaaagt atactctccg
     6301 cactccgaga tgaaaaccgg atggcgtgac atgtaaacaa cttaaccgga gtttagcttt
     6361 tccgccagtg gtggcttcgc atcttttccc catgcaattc accggcaccg ccaagtacta
     6421 cctctcggac gagctcgcgc agatcgtcga catggcgctg gcgctcgaga aaccgctgct
     6481 gctgaccggt gaaccgggca ccggcaagac gcagctcgcc tttgccattg ccgaatcgct
     6541 cggcctgggc atggaaatat tgcgggcgaa gtcgaccttt cgcggcgaag aggcggcgta
     6601 cacgcaggat acagtgctgc gcctcaacga ttcgcgcttt cccgacgccg catcgggccg
     6661 caacgtgcag aacttctacg actacatcat ctgggggccg attggccgcg cgttccgtgc
     6721 tgatcagcgc aaggtactgc tgctcgacga gatcgacaag accgagtctg acgtacagga
     6781 taacctgctc gacgtgctcg aagatacgga gtttaccatc cgcgagacca acactgtcgt
     6841 gaaggcgaag cacaaaccgg tgatcatcat cacctcgaac gcgaaacgtg aactctccga
     6901 cccgtttctg cgccggtgtt tctgccacca tatcagcttt cccacgatcg acgagatgcg
     6961 ccatatcgtc gcgctgcatt ttcccgattt tgggggtcct gtcctcgaag cagcgctcaa
     7021 aattttctac gagttgcgcg cgcgcggcta cgaaaaagct ccggccacca gcgaactcat
     7081 tgcgtggatc gaagccatcc ggcgcaaagg acttaccccc gaagaggtgg agcgtacccc
     7141 gttacccggc attctgctca agcgcgcggc agacctgctg tcgttccggg gtgaaaagcg
     7201 cgtgcggggt taatgttcct taacttcttc tacgccctgc gcgacgaagg cttgccgatc
     7261 ggcgccgacc aggtactcga attctaccgt gcgcctgaaa aggtaccggt cgaaaacacc
     7321 gatgacctct ttctcttgct gcggctcatc tgcgtgaaga aacgtgaaga tctggatacc
     7381 ttcgaacgcg tgtttctaaa ccacttctac aacatggata tcccgcgcgt cgccgagggc
     7441 gattatgccc tgctcgaaac gcggcagttt cgcgagtggc tgcgcgaggc ccgcgagcgc
     7501 ggagagatac cgtttcgcca gcatgagctc aacctcgatg agctcatgaa gaagttctgg
     7561 gaaacgctgc gcgaacagat gaaagaacac cacggcggca gcaaatgggt cggcactggt
     7621 ggcaactcgc cgtttggcca ttcgggccat tctgccgggg gcgtgcgcgt cttcggccag
     7681 ggacgcaatt tctcggcaat gaaggtgatc ggcgaaaagc ggcatgtctc atactcttcg
     7741 cgccagtcgc tcagcgcaga caacgtgcgg caggcactcg cgctgctcaa gaacatgcag
     7801 aagcatgccg cagcgaccga tctcgacatc gaagagagcg tctacctttc tgaaaaatcg
     7861 ggcgacatcg agctcgtttt ccccccgccg ctcaggaacc agctcaaggt agtgcttttt
     7921 atcgacaacg gcgggcattc gatgctgcag catgtgccgc tgacgcgcct catt
//
LOCUS       DAOQJF010000132         7958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4932_length_7958_cov_5.693787, whole genome shotgun sequence.
ACCESSION   DAOQJF010000132 DAOQJF010000000
VERSION     DAOQJF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4932_length_7958_cov_5.693787"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..516)
                     /locus_tag="PLF85_08360"
     CDS             complement(160..516)
                     /locus_tag="PLF85_08360"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE19717.1"
                     /translation="MQLREYERMFKALSDKQRLRILALLSRRPLVVNDIKLMLRLSMP
                     TISQHLSILRDADLVIDNKQGRWVEYSIHPQLHDMKTFPGIIYAELKSHFDGDDTLQL
                     DFEFLDKINSRAGTTS"
     gene            736..1611
                     /locus_tag="PLF85_08365"
     CDS             736..1611
                     /locus_tag="PLF85_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE19718.1"
                     /translation="MKTMRKWFLLAALAALAACGKKDASLKIAGSDTMVQLGQSLVKA
                     YMAANPGKSASVQGGGSGTGIAALLNGSTDIASASREMKPKEWDLAKSKGINIKEYVI
                     ALDALAVVVHPSNKVSKLTIAQLSDIYAGKITNWKEVGGADKEILVISRENNSGTHVY
                     FKEHVLRQGKEAKAGDTNPDPLARLEFGSKVTFGVSSQAISDQVKSNPAAIGYYGMGW
                     LTKDVKPLEISKDGKAFYAPTAENTSNKKYPIARGLQMYLNTAAAEKGQPFIDFVMSA
                     AGQKVVKDQDFVPVK"
     gene            1720..2604
                     /gene="pstC"
                     /locus_tag="PLF85_08370"
     CDS             1720..2604
                     /gene="pstC"
                     /locus_tag="PLF85_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNE19719.1"
                     /translation="MEKRSRITEFLVEKGLIRFSGYAAVLVLFLILLFILREGLPFLG
                     IYSLSDFLFKTDWYPLSGRYGMLPLVTGSVMVTFISLVLAVPMALGTAIFMSELAPPA
                     ARNVFKVLLEILASVPSVVFGFLGIVILGPWVQKTFGLPTGLNAFSAGILLAFMALPT
                     IASVADEAMQAVPRSLREASLALGASRMQTILRITFPAASSGVIAGVMLGIGRAIGET
                     MTVMMVAGGSAQIALSPMAPVRTMTGTIAAEMGEVVMGDDHYRALFMIGLFLFLITLG
                     VNTLSGVIIERIRRRQGT"
     gene            2601..3458
                     /gene="pstA"
                     /locus_tag="PLF85_08375"
     CDS             2601..3458
                     /gene="pstA"
                     /locus_tag="PLF85_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNE19720.1"
                     /translation="MTTRNRRFSDVIGYSVIYALTAAAFLPLAAFIIYIIVKGISRIN
                     LEFLFSASSSMESGGIFAPLYGTVLLTLVSLAIAVPISLLAAVYLSEYSRKDRWFQLI
                     ELTIVNLAGVPSVVYGLFGLGLFVIFFGLGKSIIAGALTMACMSMPLLITVFYQALKA
                     VPVTLREASLSLGASKLSTTFRIVVPTALPGIFTGVILATARIAGETAPVLFTVAAYY
                     MPNLPQSLSDQSMLLSYHLYVISTQNTGVSDEQKFAVTLVLVALVLVVNLLAIWARAR
                     ARKKIGYLG"
     gene            3461..4240
                     /gene="pstB"
                     /locus_tag="PLF85_08380"
     CDS             3461..4240
                     /gene="pstB"
                     /locus_tag="PLF85_08380"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNE19721.1"
                     /translation="MENTAETAQTILKLEKLNLHYGDKHVLKGIDMEIAPNSITALIG
                     PSGCGKSTLLRCLNRMNDLIESTKIEGKIIFDGKIDINDRRLDVTQLRRKIGMVFQKP
                     NPFPKTIYENIAYGIRIASKVPKSEVDDRVEDALKKVGLWKEVSDRLKDSGMALSGGQ
                     AQRLCIARTIATRPDIILMDEPTSALDPISTQTIEELTLELKENYTVVIVTHNMQQAA
                     RISDSTAFFYLGEMIEYSATKMMFTRPTQKRTEDYITGRFG"
     gene            4261..4887
                     /gene="phoU"
                     /locus_tag="PLF85_08385"
     CDS             4261..4887
                     /gene="phoU"
                     /locus_tag="PLF85_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNE19722.1"
                     /translation="MLIIEKALKQLRENLQSMTEVVLDMLELVHTSLEKENINDLEVV
                     FKQDKHLDQLEKSLDRQCMELLALINPHASDLRFVFSVIKTNVDLERMGDEMKNVARE
                     MKNAGEKVPPEMKELARMVSETARKTFRALANSDSKMAMEIILEDDQIDTMEYNIVQK
                     YPQLGISLTAKALERIADHATNIAENIIYSVEGIDVRHENSIRQRLAK"
     gene            4950..6269
                     /locus_tag="PLF85_08390"
     CDS             4950..6269
                     /locus_tag="PLF85_08390"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19723.1"
                     /translation="MTSEKLTELNSTAINHEDEIHRAYLRARKIIEQREWRRSIKENT
                     VFSAFEVGKLADIKIDWISAETSLKLLDDLIHDFNTEFFPVKDKGNFIGYVKAEKFRA
                     LLGQNQYSRNIFLREENRVTEVLDYGLVCVDARTPLPEVSRRLMARAREQLYDPFVIT
                     YHGEYLGVATVKAVMDGIAFYEQKDIAAAREAQQSMNNPHRNSPQIFADYDFFLEQLG
                     EVGGDFVYVQGLQPHLALFSIMDVCGKGLKAAQMAMAMGAYFRAMFRYLTRTAKEADH
                     RTVKLSARLRLLNLMLVKSTPSDMYASGVVLLLDLKNSAVLYYDFGHTPVYVLRNGRV
                     LKLPRAHHHEADGFPFLGIDPEMVIRGATVQVRSGDMIIATTDGISETRNEAREEFGE
                     EGIIRSLTGKKFHHPAELTEYMRDTLAAFRGKYRRLDDMSLLAFMVP"
     gene            6292..7110
                     /locus_tag="PLF85_08395"
     CDS             6292..7110
                     /locus_tag="PLF85_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE19724.1"
                     /translation="MPQKDKRYQFKKDTVYDALYISDIHYLLRKKVINHRHKDLFRLL
                     DHLRKKGVRFRKIFLVGDIIENWYFSSQRKLVRKFGRKRFNKLFDRIDALATPVSLKV
                     YLIGNHDSFSYISSLPPKIDQYLRERDYVISESWENDRLVVLHGHQGQYGKIFWFVNI
                     LGVRLLHMVSSVFPSVFTVMENFYRKHFNFDRNETEDEVRDYYGVLRERANQGNRLLI
                     CGHTHQPMANAALRVINTGDWIESGTFVVEHNGEFSGLQFKSKKKIHEIFNLKP"
     gene            complement(7112..>7958)
                     /locus_tag="PLF85_08400"
     CDS             complement(7112..>7958)
                     /locus_tag="PLF85_08400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE19725.1"
                     /translation="GMYVNPFRLLKLDKLLNRVKRRKKIASKLHKVTRKKPGSLGGKL
                     LYKLANRLYEKCSVIISPSELIKRELIDKGIKAPVTVVSNGIDLGFFRAERRIFPGDE
                     PRLLHVGRIAPEKNTEEVIRAFALIKRELPQARLTVVGDGPVLVDLKREASELGVGDS
                     VQFTGYMPRKELPQMYAAHDLFITASAMETQGLVALEAIATGLPAVGVNAFALPELIQ
                     QGRNGLCAEAHNVPDLAAAALRLLKQRSFFESASLESMEIAKDHGHDRCTDRLEKLYR
                     KAAGK"
BASE COUNT         1901 a         2173 c         2090 g         1794 t
ORIGIN      
        1 tcgccgcgaa ttcctccgca aattcgtcga atttgctcca cggggcgcct tgcaggcgcg
       61 aattcccggc gatttttcgc ctgtgcagcg aaaccgggca tctgcattcg cagaaaaatt
      121 gctttgtgat tcagctatcg cgacctgccg cgcagcccct taactggtcg tgccggcccg
      181 gctgttgatc ttgtccagga attcgaaatc gagctgcagg gtatcgtccc cgtcaaaatg
      241 gctcttaagc tccgcgtaga tgataccggg gaatgtcttc atatcgtgga gctgggggtg
      301 tatggaatat tctacccacc ggccctgctt attgtcgatc acgagatcgg catcgcgcag
      361 aatagaaaga tgctgcgaga tggtcggcat cgaaaggcgc agcatcagct tgatatcgtt
      421 gactaccagc ggacggcgcg acaaaagcgc aagaatgcgc aggcgctgct tatccgaaag
      481 cgccttgaac atgcgttcgt attcgcgcaa ttgcacattt aacaaatcgg cgaaatgagg
      541 caggatgtca agtaactcga atgtttccat ctttattaat tgactaacat attcatactt
      601 cgctatttat caaaataaag attttcgacc tgttgccata ggatttcatg gtccaggcac
      661 ttttggctga actccggtga tacgagatgc tttgaatccc gcatcaccgg agtttatccg
      721 tgccaaggag aatacatgaa aactatgaga aaatggtttc tgctcgcggc attggcagca
      781 ctggccgcct gcggtaaaaa ggacgcgagc ctgaaaattg cgggttcgga caccatggtc
      841 cagcttggcc agagcctcgt taaggcctac atggcagcga atcccggtaa gtcggcatct
      901 gtccagggtg gcggcagcgg taccggcatt gcagccctgc tcaacggttc gactgatatc
      961 gcaagcgctt cacgcgaaat gaaacccaaa gagtgggacc ttgccaaaag caaaggcatc
     1021 aacatcaagg aatacgtgat cgccctcgat gcgctcgcag tcgtcgtaca cccctcgaac
     1081 aaggtatcga agctgacaat cgcgcaactg agcgatatct atgccggcaa aatcaccaac
     1141 tggaaagaag tcggtggagc tgacaaagaa atcctcgtca tatcacgcga gaacaactcg
     1201 ggcacccatg tgtacttcaa ggagcacgtg ctgcgccagg gcaaagaagc caaggctggg
     1261 gacacaaacc ctgatccgct cgcaagactc gaatttggct cgaaagtaac cttcggcgtc
     1321 tcgtcgcagg caatcagcga ccaggtcaag agcaacccgg ccgcgattgg ttactacggc
     1381 atgggctggc tgaccaaaga tgtcaaacca ctcgagatca gcaaggacgg caaagcgttt
     1441 tatgcaccga cagcggaaaa tacctcgaac aagaaatacc ctatcgcccg tggcctgcaa
     1501 atgtacctga acaccgccgc ggcagagaaa ggccagccgt tcattgactt cgtcatgagc
     1561 gcagccggtc agaaagtcgt gaaagaccag gacttcgttc cagtcaagta attaccctcc
     1621 cgggtggcct cgccacccgg gattcgcatg aaagttattt agttaagtaa cgaataaaac
     1681 tatacgcaag gtcagattat cccatcttgg tagcaggtta tggaaaaacg cagccgaatc
     1741 actgaatttc tggttgaaaa gggcctgatc cgtttttccg gctatgcggc cgttcttgtc
     1801 ctattcttaa ttcttctttt catactgcgt gagggcctgc catttttagg catatacagc
     1861 ctttctgatt tcctctttaa aaccgactgg tacccgcttt cgggccgtta tggtatgctg
     1921 ccactcgtta ctggcagcgt catggtgaca ttcatctcgc tggtcctggc agtgccgatg
     1981 gctctgggga ctgcaatctt catgtcagaa ctcgctccac cggcagcccg caacgtattt
     2041 aaggtcctgc tcgaaatact ggcatcggta ccttcggttg ttttcggatt tctaggtatt
     2101 gtcattcttg gtccctgggt gcagaaaaca tttggcctgc cgacgggcct caatgcattc
     2161 agcgctggca tactactggc attcatggcc ctgccgacga tcgcttcggt cgctgacgag
     2221 gccatgcaag ccgtaccgcg cagcctgcgc gaagcctcgc tggcgctcgg tgcaagccgt
     2281 atgcagacga tcctgcgcat tactttcccc gctgcttctt cgggtgtcat tgcaggcgtt
     2341 atgctcggca ttggccgcgc gattggtgaa actatgacgg tcatgatggt cgcgggcggt
     2401 tcagcgcaga ttgcgctttc gccgatggcg ccggttcgta cgatgacggg taccatcgcc
     2461 gccgagatgg gtgaagttgt catgggcgac gaccactacc gggccctgtt catgattggt
     2521 ctctttttgt ttctgattac gcttggcgtc aatacgctga gcggtgtgat aattgagcgc
     2581 attcgccgca ggcagggtac atgaccactc gtaaccgacg tttttctgac gttattggct
     2641 actctgttat ctatgcgctg acagcggcag cttttctgcc gctggccgcg tttattatct
     2701 acattattgt aaaaggcatc agccgtatca acctggagtt tctcttcagc gcatcgtctt
     2761 cgatggaaag cggcggtata ttcgccccgc tctacggcac ggtcctgctg acgctggtgt
     2821 cactggcgat cgccgttccg atatcgctgc tggcggccgt ctatctctct gaatattcac
     2881 gcaaagaccg ctggttccag ctgatcgagc tgaccatcgt caacctggcc ggggtgccgt
     2941 cggttgttta tggcctcttt ggtctcggct tgtttgtcat tttctttggc ttaggcaaat
     3001 ccatcatcgc gggcgcactg acaatggcct gcatgtcgat gccgctattg atcaccgtct
     3061 tttaccaggc cctcaaggca gtgccggtta ccctgcgcga ggcatcactg tcactcggcg
     3121 cgagcaaact ctcaacgaca ttccgcatcg tggtaccgac agccctgccg ggtattttta
     3181 ccggcgtcat tcttgccacc gcgcgcatag cgggtgaaac tgctccggtg ctttttaccg
     3241 tcgcggcata ctacatgccc aacctgccgc aaagcctgtc agaccagtcc atgctgctct
     3301 cttaccacct ctatgtcatc agtacgcaga atacgggcgt cagcgacgaa cagaagtttg
     3361 ccgtcacgct cgtcctggta gcgctggtac tggtggtgaa cctgcttgcc atctgggcac
     3421 gcgcccgcgc gagaaagaaa atcggatacc tgggataatt atggaaaata ccgctgaaac
     3481 cgctcagaca attctgaaac tggaaaagct gaatctgcac tacggggaca aacacgtcct
     3541 gaagggcatc gacatggaaa ttgcgccgaa ctcgattacg gcgctcatcg gcccttctgg
     3601 ctgcggcaag tcgaccctgc tgcgctgcct gaaccgcatg aacgacctga ttgagtcgac
     3661 aaaaatcgaa ggcaaaatta ttttcgatgg caagatcgat atcaacgatc gcaggctcga
     3721 tgtcacacag ctgcgccgca agatcggcat ggtattccag aaacccaacc cgtttccaaa
     3781 aaccatctac gaaaacattg cgtacggtat acgcattgcg agcaaggtgc caaaatccga
     3841 ggtcgatgac cgggttgaag atgcgctcaa gaaagtcggc ctttggaaag aagtctctga
     3901 ccgtctgaaa gattcgggca tggcgctctc gggtggccag gcgcagcgcc tgtgcattgc
     3961 acgcactatc gcaacccgcc ctgacatcat cctgatggac gaacccacga gcgcgcttga
     4021 cccgatcagc acgcagacga tcgaagagct cacccttgaa ctcaaggaaa attacacagt
     4081 ggtcatcgtt acgcacaata tgcagcaggc cgcgcgtatc tcagacagta ccgcattctt
     4141 ctacctcggc gagatgatcg aatacagcgc gacgaagatg atgttcaccc gcccaacgca
     4201 gaaacggacg gaagattata ttaccggaag gtttggttaa taccggtcac aaggagccat
     4261 atgctgatta ttgaaaaagc cctcaaacaa ctgcgcgaaa acctgcagtc gatgacagaa
     4321 gttgtactcg acatgctgga actcgtgcat acttcgctgg agaaagaaaa catcaacgat
     4381 ctcgaagttg ttttcaagca ggataagcac ctcgaccagc tggagaaatc cctcgatcga
     4441 caatgcatgg aacttctcgc gctgatcaat ccgcatgctt ctgacctgcg cttcgttttt
     4501 tccgttatca agaccaacgt cgacctcgaa cgcatgggtg acgaaatgaa gaacgttgcg
     4561 cgcgaaatga aaaatgccgg cgaaaaagta ccgccagaga tgaaagaact ggcgcgcatg
     4621 gtttcagaaa cggcacgaaa gacattccgc gccctggcca acagcgattc caagatggct
     4681 atggaaatca ttctcgaaga cgaccagatt gatacgatgg aatacaatat cgtgcagaag
     4741 tacccgcagc tgggaatttc gctgaccgcg aaggcactcg agcgcattgc cgatcatgcg
     4801 acgaacattg ccgaaaacat catttattcg gtcgagggaa tcgacgtgcg ccacgaaaat
     4861 tccattcggc agcgactcgc aaaataggcg gagcttagtg cggcacaaaa aggttgccgc
     4921 gcgatgatta tttcgttaat ttgctaaata tgacatctga aaaactgaca gaactcaaca
     4981 gcacagctat aaaccacgaa gatgaaatac accgcgccta cctgagggca cgaaagatta
     5041 tcgagcaacg cgagtggcgc cgttcgatta aagagaacac ggtcttttca gcttttgaag
     5101 tcggcaagct ggccgatatc aagatcgact ggatctccgc cgaaacgagc ctgaagctgc
     5161 tcgatgacct gatccacgat tttaacacgg aattctttcc ggttaaggac aagggtaact
     5221 tcatcggtta cgttaaggcc gaaaaattcc gggcattact cgggcagaac cagtatagcc
     5281 gcaacatatt cctgcgcgaa gaaaacaggg tgaccgaagt gctcgattat ggcctcgtct
     5341 gtgtcgatgc gcgcactccc ctgcccgaag tttcacggcg gttgatggcc cgcgcacgcg
     5401 agcaactcta cgatccgttt gttattacct accacggtga atacctcggg gtagccactg
     5461 tcaaagcggt catggatggc atcgcatttt acgaacagaa agatattgct gccgcgcgcg
     5521 aagcgcagca gtcgatgaat aatccacacc ggaacagccc gcagattttt gccgactacg
     5581 acttctttct cgagcagctc ggcgaagtgg gtggcgattt tgtctacgtc cagggactgc
     5641 agccgcacct ggcgcttttt tccatcatgg atgtctgtgg caaaggcctc aaagccgcgc
     5701 agatggcgat ggccatgggc gcctactttc gcgccatgtt tcgttacctc acgcgtaccg
     5761 cgaaagaggc tgaccacaga acggtgaaac tctctgcgcg gctgcgtttg ctcaacctga
     5821 tgctggtgaa atcgacacct tcggacatgt acgcatcggg tgtggtgctg ttactcgacc
     5881 tgaagaacag cgccgtcctg tactacgact ttgggcacac cccggtttat gtactgcgca
     5941 atggccgggt gctgaaactt ccccgggcgc atcaccatga ggcagacggc tttcccttcc
     6001 tgggcattga cccggaaatg gtcatacgcg gtgccacggt acaggttcgc tcaggcgaca
     6061 tgatcatcgc aaccacggac ggcatcagcg aaacccgcaa cgaagcgcgc gaagaattcg
     6121 gtgaagaagg tatcatccgt tcactgactg gaaaaaaatt ccaccaccct gcagaactca
     6181 cggaatacat gcgggataca ctcgccgcgt tccgtggcaa ataccgaaga ctggacgata
     6241 tgtcattact ggccttcatg gtgccgtagc gctggccgca gagcagacaa agtgccgcaa
     6301 aaagataaaa gataccaatt caaaaaagat accgtctatg acgctcttta tatatccgat
     6361 attcactacc tgctgcgcaa gaaagtaatc aaccaccgcc ataaagatct cttcaggctg
     6421 ctcgaccatt tgcgcaaaaa aggcgtccgc tttcgcaaga tttttcttgt gggagatata
     6481 attgagaact ggtattttag ctcgcagcgc aagctggtcc gcaagttcgg gcgcaaacgc
     6541 ttcaacaagc tctttgaccg catcgatgcg ctcgcgacac ccgtatcgct aaaggtctac
     6601 cttataggca accacgactc gtttagctac atcagcagcc tgccccccaa aatcgatcag
     6661 tacctgcgtg aacgcgatta cgtgatcagc gaatcgtggg agaacgaccg gctggttgtg
     6721 ctgcacggcc accagggcca gtacggcaag atcttctggt ttgtgaatat tctcggggtt
     6781 cgcctgctgc acatggtaag cagcgtcttc ccttctgtat tcacggttat ggaaaatttt
     6841 taccgcaagc acttcaattt tgaccgcaat gaaacagaag acgaggtgcg cgactattac
     6901 ggtgttctgc gcgaacgagc caaccagggt aaccggcttc ttatctgcgg gcacacgcac
     6961 cagccgatgg ccaacgccgc actacgcgtc atcaacaccg gcgactggat cgaatcaggt
     7021 acgtttgtcg tagaacacaa cggggaattt tcaggcctgc aattcaagtc aaagaagaag
     7081 atccacgaga tcttcaacct gaaaccctaa tctattttcc cgccgctttg cggtagagtt
     7141 tttccagccg gtcggtgcag cggtcgtggc catggtcctt cgcaatttcc atgctctcga
     7201 gtgaagcact ctcgaaaaag gacctctgtt tcagcaggcg cagcgctgcc gcggcaaggt
     7261 cgggcacgtt gtgtgcctcg gcgcagaggc cgttgcgacc ctgctgtatc agttcgggta
     7321 gcgcaaacgc gttgacgcct accgctggta ggccagtcgc gatggcctcg agggcgacaa
     7381 gtccctgggt ttccatggca gaggccgtga tgaataggtc gtgggcagca tacatctgcg
     7441 gcaactcttt gcggggcata taccccgtga attgaacgct gtcgccaacg ccgagctcag
     7501 atgcttcgcg cttgaggtcc acgagaaccg ggccgtcgcc caccacggtc aggcgtgcct
     7561 gcggcagttc acgcttgatc agggcaaaag cgcggatcac ttcttcggtg ttcttttccg
     7621 gggcgatacg gccgacatgc aggaggcgtg gttcgtcacc ggggaaaatc cgcctctctg
     7681 cgcgaaagaa acccaggtca atgccattgg aaacgacggt cactggggcc tttataccct
     7741 tgtcgatgag ctctctcttg atgagttcgc tgggagatat gatcacgctg catttttcgt
     7801 acaggcggtt cgccagcttg tagagcagtt taccgcccaa agagccgggt tttttacgcg
     7861 tgaccttgtg cagtttgctg gcgatctttt tacgcctttt gacccgattc agcagcttgt
     7921 cgagtttgag caggcgaaac gggttcacgt acatgccc
//
LOCUS       DAOQJF010000133         7944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4945_length_7944_cov_4.802763, whole genome shotgun sequence.
ACCESSION   DAOQJF010000133 DAOQJF010000000
VERSION     DAOQJF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4945_length_7944_cov_4.802763"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLF85_08405"
     CDS             complement(<1..331)
                     /locus_tag="PLF85_08405"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNE19726.1"
                     /translation="MQELPLEFFIAAHDGQSMQRAVERLAGHLKAPLLVRVVGNLGAG
                     KTTLVAALLTYWQCPGANSPTFNLRNDYASAGGRVIHLDLYRLKSTDDALDLLPPDED
                     YSDAIVFV"
     gene            469..852
                     /locus_tag="PLF85_08410"
     CDS             469..852
                     /locus_tag="PLF85_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNE19727.1"
                     /translation="MVKIGQRQTRQRAIIHDIIMESDRPLSIANLVTLAQEKQAKIGQ
                     ATVYRTVNALLEASEIQTVQMPGGEALYEKAHLHHHHHFKCNSCGKVFDLPGCPEKDL
                     QKFLPRGFKMTHHEFTFYGTCADCA"
     gene            complement(825..1295)
                     /locus_tag="PLF85_08415"
     CDS             complement(825..1295)
                     /locus_tag="PLF85_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HNE19728.1"
                     /translation="MAGLAENMKGRPRRRAFFALIWDKLGIVVSGLCLVDCLVLPLLS
                     ALLISLQSSVSWFANLHFYLLPVIGVTASMAFHHSYKAHRSYGIVIMGAVGYVLLVLG
                     EVFEAKLRFFLFNWVTLLGSALLIAAHLRNLLMHTGHRHKHAHPAGHAQSAHVP"
     gene            complement(1348..2274)
                     /locus_tag="PLF85_08420"
     CDS             complement(1348..2274)
                     /locus_tag="PLF85_08420"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNE19729.1"
                     /translation="MRWFIYAFCAALSLPGCRAELKEAAHSPLARIRLPSGSYVMTHG
                     NRQRRYHVHLPKAREHEVKLPLVLALHGAPGHGANMMEFSGVRELADRKGFIAVFPDG
                     TSAQDPVFLNWNSGTCCGYARDNNIDDVGFLRRLIELLLAEYEADSGRVYVTGFSKGG
                     MMAYYFACQHANLLAGIAVVAGAFNLSECHPARALDVLIVHGRADAAIPYGGELPRKI
                     PPLRETEDRPVSYATHFWRKVNHCHRAASSSAGTVEVTDYHCFRAPLRLISLSDEGHT
                     WPGAADGLIGAEKPRGQFLVGEAIWSFWQGLP"
     gene            2353..3693
                     /locus_tag="PLF85_08425"
     CDS             2353..3693
                     /locus_tag="PLF85_08425"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19730.1"
                     /translation="MRQNLLSRFSDLFWQNPVSTSPAVAEVFNREEVRGARALLYCRL
                     GVVLLLVIGGVSAPSRWQDKLSNMVFAAAYAAVVGVQLYLHRCNNLRALHRFSYFSLL
                     ADHAVFTFQILFYYQNEGMGNFNFALKSPYVVLLLMPTLTTLLQFRFSLLVFSLGTSL
                     AILLGFFGFALLSRVPQSPSWNQYFLGETIILPAWLGLWLLLPLFTAGLIAYSILRSR
                     RMVAEIGQKENQKRQLARYFSPAVAEEIAAAGDGKAMRGERRQVAVLFADIRSFTSLS
                     EHLPPDEIAAMLSELRQLQTSAVFRFGGTVDKFIGDALMAVFGAPKSSGNFVQDIHNS
                     VSCALAILGDLEEFNARRTAAGKESIRIGIGIHAGEVFAGNLGSEDQLEYTVIGDVVN
                     TASRIEHLCKKAQAEFLVSEIVARELPEHVITERLGVVKIRGREQPMGIYRVHR"
     gene            complement(3757..4200)
                     /locus_tag="PLF85_08430"
     CDS             complement(3757..4200)
                     /locus_tag="PLF85_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19731.1"
                     /translation="MLLRLIYIFLVPVFVSCIQSTKITLEYINPPISLAITQIAAQTY
                     QISFYSDNREGGFVGYGIFTAGSSSSASVYPANDIATAAVFCTNSGQINYKTTVTIEV
                     GPSAAGGSICNDTGLVLTSGQYIALRGRVERTEKPWSEAAVAQVP"
     gene            4262..5563
                     /gene="purD"
                     /locus_tag="PLF85_08435"
     CDS             4262..5563
                     /gene="purD"
                     /locus_tag="PLF85_08435"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNE19732.1"
                     /translation="MRILILGSGGREHALYRRIKQSTRAEKVFVWPGNGLVPAADRVG
                     QQAIVADFSELEKFIAAERIDFTVVGNEAPLVEGVRDHLKKVMPGHFVFGPDRLSAQL
                     EGSKAFSDNFMREAGIPHGESIIAESLGDALKALPAQALPVVIKADGLAAGKGVSIHN
                     DYASAEEKLREIFDGRIFGDSGNRVLLQRFLRGTEASLFAICNGSDAILLPVARDYKR
                     ALDNDEGDNTGGMGAYCPGEHLTEAQKQFVRTKIIQPVLEKFQYHGLLYVGLMIASEH
                     ADDLAVVEFNCRFGDPETQSIMHLVEGDMLDYLLWSDGYDVKFPLLQSGPAKLVPYRR
                     GAVVNIVIAAKGYPGSFAKEIPLTFPEYRNNELHVTGAGLIERDGALFSTSGRIANVV
                     AYGPNREAARALAYDYLQHFAAANSAQADKLYWRTDIAAGI"
     gene            5569..6360
                     /locus_tag="PLF85_08440"
     CDS             5569..6360
                     /locus_tag="PLF85_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19733.1"
                     /translation="MQTLGEILRQKDPAYLRYLSRKTQYLITHEKTEFVQRFWQHLVM
                     DYHPSVVLPRLNATERKTFHQLLQQFARDDRATMRARYQKLEKKIPWLLQHPDGGFFI
                     PFEIIKTLMPKKGLIPEGYLFQLLYAMPEAEQHSLRALLARSHRSREALGHEKHQLDR
                     ALALYIWTADHRGTAKRQKHTQARSIWQHLEEMFPKQQAEIEEWQHVMLTGRKGFYRS
                     LLLVRGAELLKRYISGLRVVPVATRRHKVFPAANLKYVVPFEFRP"
     gene            complement(6372..>7944)
                     /locus_tag="PLF85_08445"
     CDS             complement(6372..>7944)
                     /locus_tag="PLF85_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19734.1"
                     /translation="FFDRAIKFDERNFYAHYNMAIALRHKGMYKEALQALERAQKLRP
                     DLVEPQSLKGQLQYQNQDLKGAEATLKQAIETSKDPLAYYNLGMVYKKDGKFAEAKAS
                     FLDALNVAASGEIAFKAATQLGMLHATQGDLPNAKYYFERAVALSPANAKYHYNLALI
                     LNQMGENERAVRELDEAIRLGSDNPQTYMYIARLYSELGRKEQAEAALAKALAEAPRD
                     PVILSQMGDMLVAQAKWAPAIRIFKELYESSPRTAEKAQAMYNLGKVYVALRDFRNAE
                     KALETAYQLDMLNEDALVLLGEAYVQTGQAHKSVTLFKEALRLNPDNLKVLRGQAQLY
                     LNIGELTEAEAALKRLAEHPNRRADDAYFAAQMLGDLYMKRKAYDTAMQYYEKASQST
                     DANLKYTTLLSQADAMLLSDRPAQMVYPLLNQAIALRPNADEARMLLARALMREGTIS
                     SQDRAIEELRAVTTDVRSVPLASKAYTLMGIIWYKQGQLNRSLDAFNRALELDPANGE
                     AFQNRRAVSDRLENG"
BASE COUNT         1734 a         2295 c         2257 g         1658 t
ORIGIN      
        1 cgacaaacac aatggcatcc gagtagtctt catcgggggg cagcaggtcg agcgcgtcgt
       61 cggtagattt cagccggtac aggtcgagat ggatgaccct gcccccggcc gaagcatagt
      121 cgttgcgcag gttgaatgtc ggtgagttcg cgcccgggca ttgccagtag gtgagcagcg
      181 ctgccaccaa cgttgttttg ccagccccaa gattgcccac aacacgcacc agcagcggag
      241 cttttaggtg ccctgcaagg cgttcaaccg cacgctgcat actttggccg tcgtgcgctg
      301 ctatgaaaaa ttcgagggga agttcttgca cgaggtagat caacgcagag ttcggggcgc
      361 aagacagagt gccaaccagt tttgcccggc ccgcgtgtcc tggcggtgtt ccgggtaatt
      421 tccaattgca gccttgtgtc ggctttgggc aatctgccac cgagccctat ggtcaagatt
      481 ggacaacgcc agacgcgcca gcgcgccatc atccatgaca ttatcatgga atctgaccgc
      541 ccgctgagta tcgccaacct ggttaccctg gctcaggaaa aacaggcaaa aatcggtcag
      601 gcaacagtat accgaacggt caacgcgctg ctcgaagcca gcgaaataca gacggtgcag
      661 atgccgggcg gcgaagcgct ttatgaaaag gcccacctgc accaccacca ccatttcaag
      721 tgtaacagct gtggcaaggt gttcgacctg cccggctgcc ctgaaaaaga cctgcagaaa
      781 tttttgccgc gcggctttaa gatgactcac cacgaattca ctttctacgg tacgtgcgca
      841 gactgcgcat gacctgcagg gtgcgcgtgc ttgtgccggt gtccggtatg catcagcagg
      901 ttacgcaggt gcgccgcaat cagcagggct gaccccagca gtgtcaccca gttaaacagg
      961 aaaaagcgca attttgcctc aaagacctca cccagaacca gcaggacgta gccgactgca
     1021 cccatgataa cgataccata cgagcggtgg gccttgtagc tgtggtgaaa ggccattgaa
     1081 gcagtgactc caataaccgg cagcaggtaa aagtgcaggt tggcaaacca gctgaccgaa
     1141 gattgcaggc tgatcagcag ggcagaaagc aggggcaaaa caaggcagtc aaccaggcag
     1201 agccctgaaa ccacaatgcc gagtttatcc cagatcagcg caaagaatgc gcggcgacgc
     1261 ggtctgccct tcatattctc tgccaacccc gccataacga taacgaaata tcattatatg
     1321 tactgtaaag gacaaatgcc tcttctgtca gggtaacccc tgccagaatg accagatagc
     1381 ctcacccacc agaaattgcc cgcgcggctt ctctgcccct atgagaccat ccgcagcccc
     1441 cggccaggtg tggccctcgt cgctaagact gataagtcgc aagggtgcac gaaagcaatg
     1501 gtaatcggtt acctcgactg taccggcaga actgctcgct gcacgatggc agtggttcac
     1561 cttgcgccag aaatgcgtgg cgtatgagac aggccggtct tcggtttcgc gcagcggcgg
     1621 tatcttgcgc ggcaattcac cgccgtaagg aatcgcggca tcggcccggc cgtgcacaat
     1681 cagcacgtcg agtgcgcgcg caggatggca ctcgctcagg ttgaacgcgc cggcaacaac
     1741 ggcaattcca gccagcaggt tggcatgctg gcaggcgaag taatacgcca tcatgccacc
     1801 tttggaaaac ccagtaacat aaacacgccc gctgtctgct tcgtactcgg ccagtagcag
     1861 ttctatcagc cgccgcagga agcccacgtc gtcgatgttg ttgtcgcgcg cataaccgca
     1921 gcaggtacct gaattccagt tgagaaagac aggatcctgt gcgctggtgc catcggggaa
     1981 aacggcgata aaacctttgc gatcggccag ttcgcgtacc ccggagaact ccatcatgtt
     2041 cgcgccgtga cctggcgcac cgtgcagcgc cagaacaagg gggagcttca cttcatgctc
     2101 tcgcgctttc ggcaggtgca catggtaccg ccgttgccgg ttaccgtgag tcatgacata
     2161 actgcccgat ggcaggcgaa tccgtgccag cggtgaatgc gccgcctctt tgagttcggc
     2221 ccggcagcct ggcagcgaaa gtgcggcgca gaatgcatag atgaaccagc gcacaaaaac
     2281 ccgtaccgtg aggacactca gagagccaac aaaaatcggt tgaagaaatg cgcgtgagcg
     2341 taaccccggt gcatgcggca aaacctgctc agccgattca gcgacttgtt ctggcagaac
     2401 ccggtgagca ccagcccggc ggtggccgaa gtttttaacc gcgaagaggt gcgcggtgcg
     2461 cgcgcactgc tctactgtcg cctcggtgtg gtgctcctgc tggtcatcgg tggcgtaagt
     2521 gcgccatcac gctggcagga taaactctct aacatggtgt tcgcagcagc ctacgctgcg
     2581 gtcgtcggtg tgcagctgta cctgcaccgc tgcaataacc tgcgcgcgct gcaccggttc
     2641 agctactttt cactgctggc cgaccacgcg gttttcactt tccagattct cttctactac
     2701 cagaacgaag gcatgggaaa ttttaacttc gcgctgaaat cgccttacgt tgtgctgctg
     2761 ctgatgccga cgctgaccac gctgctgcag tttcgctttt ccctgctcgt gttttccctc
     2821 gggacgtcgc tggctatcct gctcggcttt ttcggttttg cgctattgag ccgggtaccc
     2881 cagagcccaa gctggaacca gtattttctt ggtgagacaa tcattctgcc agcctggctc
     2941 ggtctctggc tgctactacc gctcttcacc gccggcctga ttgcctatag tatcctgcgc
     3001 tcgcgccgca tggtcgcaga aatcgggcaa aaggaaaacc agaaacggca actggctcgc
     3061 tatttttctc cggcagttgc tgaagagatt gccgctgcgg gcgacgggaa ggcaatgcgc
     3121 ggcgaacgcc ggcaggttgc agtactcttc gccgacatcc gctcgttcac gagcctctca
     3181 gagcacctgc caccggatga aatagcggcg atgctctctg agctgcggca gctgcagacc
     3241 agcgcggtat tccggttcgg cggaacggtc gacaagttca tcggcgacgc tctcatggca
     3301 gtcttcggtg cccccaaaag ctcgggaaat tttgtgcagg acatccataa ctccgtctca
     3361 tgtgcgctgg cgatactcgg cgacctcgag gagtttaatg caagacgcac tgctgccgga
     3421 aaagaaagta ttcgcatcgg tatcggtata catgccggag aagtttttgc gggcaacctg
     3481 ggcagcgaag accagctcga gtacacggtg atcggtgacg tggtgaatac cgcatcacgc
     3541 attgaacacc tgtgcaaaaa agcgcaggcg gagtttctgg tttcagagat tgtcgcgcgt
     3601 gaactgcctg aacacgtcat caccgagcgg ctcggtgtgg taaaaatacg cggccgcgaa
     3661 cagcccatgg gtatctaccg cgtccatcgc tgatgcgcta caagacaaca agccggagga
     3721 aagtttgaaa cggtcgtggc cgcgatacgc cagaggctac ggcacttgcg caaccgcggc
     3781 ttcagaccag ggcttttcgg tccgttcaac gcggccgcgt agcgcaatat actggccaga
     3841 ggtcagcacc aaacccgtat cgttacagat gctgccgcct gcagcagacg gcccaacctc
     3901 aattgtcacg gtggttttat aatttatctg cccgctgttg gtgcagaaaa cagctgccgt
     3961 ggcgatgtcg tttgccgggt atacagacgc tgaagaagaa ctgcccgcgg tgaatatacc
     4021 atagccgaca aaaccgcctt cacggttgtc cgaataaaac gagatctggt aagtctgcgc
     4081 cgcaatctgc gtaatagcca ggctgatcgg cggattaata tactccaagg tgatcttggt
     4141 cgactggatg cagctgacga acaccggtac caggaagatg tatatgagcc tgagcagcac
     4201 cggcttataa ttgttttttc attgtcgcag acaatgaaaa aacgttattc ccctttcctg
     4261 catgcgcata ctgatactcg gctcgggcgg ccgcgaacac gctctctacc gcaggataaa
     4321 acaatcaacc cgcgcagaaa aagtattcgt ctggcccggt aatggcctgg tacctgctgc
     4381 tgaccgcgtc ggccagcagg caatcgttgc agacttctcc gagctggaga aatttatcgc
     4441 cgctgagcgc atcgacttta ccgttgtcgg caacgaagcg ccgctcgtcg agggcgtacg
     4501 cgaccacctg aagaaggtca tgccaggcca tttcgtgttc ggtcccgacc ggctgtcagc
     4561 gcagctcgaa ggttccaagg cattttctga taatttcatg cgcgaggcgg gtatacctca
     4621 cggcgagagc atcattgcag aatcgctggg cgatgcgctc aaggcactgc ctgcacaggc
     4681 gctgccggtc gtcatcaagg ctgacggtct tgccgccggc aaaggtgtca gcatccacaa
     4741 cgactatgca tcggcagaag aaaaattacg cgaaattttt gatggcagaa ttttcggcga
     4801 ctcgggtaac cgtgtgctgc tgcagcgttt tctgcggggt accgaagcgt cgctattcgc
     4861 gatctgcaat ggcagcgatg caattctgct gcccgttgcg cgcgattaca agcgtgcgct
     4921 cgacaatgac gagggtgaca acacgggcgg catgggggct tactgcccgg gggagcacct
     4981 gaccgaggcg cagaagcagt tcgtcaggac aaaaatcatc caacccgtcc tggaaaagtt
     5041 tcaataccac ggcctgctct acgttggcct catgatagcc tcagagcacg ctgacgacct
     5101 tgccgtggtg gaattcaatt gccggtttgg cgaccctgaa acccagtcga tcatgcacct
     5161 ggtagaaggc gacatgctcg actacctgct gtggtcggac ggctatgacg tgaaattccc
     5221 gctgctgcag tcggggccgg caaaattagt gccctaccgt cgcggtgccg tcgtcaatat
     5281 cgtcatcgcg gcgaaaggtt atcccggcag ttttgcaaaa gaaatccccc tcacctttcc
     5341 cgaataccgc aacaacgagc tgcacgtcac cggtgcgggt cttatcgaac gcgacggggc
     5401 gctcttctcg acttcaggcc gtattgccaa cgtggtcgct tatggcccca accgcgaggc
     5461 cgcgcgtgca ctggcgtacg attacctgca gcacttcgct gctgccaaca gcgcacaggc
     5521 agataagctc tactggcgca ccgacatcgc ggccggtatc tgacgccaat gcagacgctg
     5581 ggcgaaatac tgcggcagaa agacccggcc tacctgcgtt acctttcacg gaaaacgcag
     5641 tacctgatca cgcacgagaa gaccgaattt gtccagcgct tctggcaaca cctcgtgatg
     5701 gattaccatc cttcggtggt actgccgaga ctcaacgcca ccgaacgaaa aaccttccac
     5761 caactgctgc agcagttcgc gcgcgacgac cgggcaacca tgcgcgcacg ctaccagaag
     5821 ctggaaaaga agatcccctg gctcttgcag catcccgatg gcggattttt tattccgttt
     5881 gagatcatca agacgctgat gcccaagaag gggcttatac ccgagggtta ccttttccag
     5941 ctattatatg caatgcccga ggccgagcaa cactccctgc gcgcgctgct tgcccgttcg
     6001 caccgttcgc gcgaggcgct tggccacgag aagcaccagc tcgaccgtgc gctcgcgctc
     6061 tatatctgga ctgcagacca tcgtggtact gcaaaacgcc agaagcatac acaggcacgc
     6121 agtatctggc agcacctgga agagatgttc ccgaaacaac aggcggaaat cgaagagtgg
     6181 cagcacgtga tgctaaccgg ccgcaagggc ttctaccgct cgctgctgct ggtgcggggt
     6241 gcagaattgc tcaagcgcta tatttccgga ttacgcgtgg tacccgtggc aacgcggcgc
     6301 cacaaagttt tccccgctgc gaatctgaag tacgtggtgc cgttcgaatt caggccctaa
     6361 caagggacct gctaaccatt ttccagccgg tcagagactg cgcggcggtt ctggaaagct
     6421 tcgccgttgg ccgggtcgag ctcgagagca cggttaaaag catcgagcga acggttgagt
     6481 tgtccctgct tgtaccagat aatgcccatc agtgtatagg cctttgaggc cagcggaacg
     6541 gaacgcacgt cggtcgtcac cgcgcgcaac tcttcaattg ctctgtcctg cgaagagatt
     6601 gtaccttcgc gcatgagagc acgggcaagc agcatacgcg cttcgtcggc gttggggcgc
     6661 agtgcaatcg cctggttgag cagcgggtag accatctgcg ccgggcggtc gctgagcaac
     6721 atcgcgtcgg cctgcgacag caacgtcgtg tatttcaggt tggcgtcggt cgactggctg
     6781 gccttttcgt agtattgcat cgccgtatca tacgctttgc gcttcatgta aaggtcgccc
     6841 agcatctgtg ctgcaaagta cgcatcatcc gcacggcggt tcggatgctc tgcgaggcgc
     6901 ttgagtgcgg cttcagcttc ggtcagttca ccgatattca ggtagagctg cgcctggccg
     6961 cgcagcacct tgaggttatc cgggttgagg cgcagcgctt ctttgaagag cgtaaccgac
     7021 ttatgcgcct ggcctgtctg aacgtaagcc tcgccgagca gtaccagcgc atcttcattc
     7081 agcatatcga gctggtaggc agtctcgagc gccttttcgg cgttacggaa gtcgcgcagg
     7141 gcaacgtaga ccttaccgag attatacatc gcctgtgcct tttcggctgt ccggggagaa
     7201 ctttcataga gctctttgaa aatgcggatc gcaggcgccc acttagcctg cgccaccagc
     7261 atgtcaccca tctgcgaaag aattaccggg tcacggggag cttcggcgag tgccttggcg
     7321 agagccgctt cggcctgttc tttgcgaccc agctcagaat agagccgcgc aatgtacata
     7381 taggtctgcg gattgtcgct gccgaggcga atagcttcgt cgagctcgcg caccgcgcgc
     7441 tcgttttcgc ccatctggtt gagaatgagt gcgaggttgt agtggtattt cgcgttggct
     7501 ggtgaaagcg ccaccgcgcg ctcaaagtag tatttcgcgt tcggcagatc tccctgcgtc
     7561 gcgtgcagca taccgagttg ggtagccgct ttaaaggcaa tttcacccga tgcggccaca
     7621 ttcagcgcat cgagaaacga agccttggcc tcggcaaact tgccgtcttt tttatagacc
     7681 ataccgaggt tatagtaagc caacgggtct tttgatgtct caatcgcctg cttgagtgtc
     7741 gcttcggcgc ccttgaggtc ctggttctgg tactgcagct ggcctttcag gctctgcggt
     7801 tcgaccaggt cagggcgtag cttttgcgca cgctcgagcg cctgcagggc ttctttatac
     7861 atccccttgt gccgcagggc gatagccatg ttgtaatggg cgtagaagtt gcgttcgtca
     7921 aatttgatcg cgcggtcaaa aaaa
//
LOCUS       DAOQJF010000134         7911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4972_length_7911_cov_5.209903, whole genome shotgun sequence.
ACCESSION   DAOQJF010000134 DAOQJF010000000
VERSION     DAOQJF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4972_length_7911_cov_5.209903"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLF85_08450"
     CDS             complement(<1..681)
                     /locus_tag="PLF85_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE19735.1"
                     /translation="MKILWIALISLTLLMACQKKPGSGDSSFYAIDSIVGANMRETPS
                     QKGKIVANLPLGTRISLLNKNGPVETIYNVTGPWCEVQAEAGQGWVFCPLLRQSDMSR
                     TKLSDDKYTKMIFDAYAKGGPQEVQKYTASLFHSVEALQKMPITPDERAKGMKDFSGD
                     YLTILDKDRQLSEQTYEQGKSENIDWNKASIVNTMVLHSEKSEIPGWWVHKVLGVQFK
                     SDGVAYKIN"
     gene            complement(784..1203)
                     /locus_tag="PLF85_08455"
     CDS             complement(784..1203)
                     /locus_tag="PLF85_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE19736.1"
                     /translation="MPASAARLFAFHERTDAFALLQPPWQTSEIIQPPTSLAVGTRVI
                     LRTRIGPVWQTIEAEHVQYEAGRMFADRMVKGPFRYWLHRHIVEPVSENESILIDDIE
                     YALPFGPVGAFFGGWFARRELERLFEYRHRVTHEACR"
     gene            complement(1233..1976)
                     /locus_tag="PLF85_08460"
     CDS             complement(1233..1976)
                     /locus_tag="PLF85_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE19737.1"
                     /translation="MAAKESYVAIDQARELSPQPPSRWGLWREAAVAIEVANLVLRYP
                     ALTRSKHTFNHPVVLFPGFGTDEVAMAVLHHYLKTIGAEVHHWGLGRNHGYVPDLLAQ
                     ALKRVQQLAEAGRPVHLVGWSLGGYVAREVARELPGQVARVVTLGSPVVGGPKFTAVG
                     KLYKSWGFDLEAMARDVERRSAVPIHNEILAIYSQTDAVVDWQACIDQRSPNVTHAEV
                     SGSHLGLVVNAKAFELVRDFLTASPEREQ"
     gene            2058..2498
                     /gene="dtd"
                     /locus_tag="PLF85_08465"
     CDS             2058..2498
                     /gene="dtd"
                     /locus_tag="PLF85_08465"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNE19738.1"
                     /translation="MVALLQRVRTAAVDIAGERVSEIDPGLLVLLGVGNEDNESNAAA
                     LAKKTAALRIFSDEAGKMNLSVADTQGEVLVVSQFTLMADTQKGNRPSFVAAAKPELA
                     IPLYEFFVAELGKILGKEIKTGRFAADMQVSLVNDGPVTIWLEA"
     gene            complement(2499..3518)
                     /locus_tag="PLF85_08470"
     CDS             complement(2499..3518)
                     /locus_tag="PLF85_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19739.1"
                     /translation="MMRIVGYILIAAGLTACTAEAQMEDFIRRNTPPPEVLPDPNLIF
                     SEDFDNGLAKWNQSSGSWTTVASGTGSLALLSPSSTTAATFSITTAAFIDLTGRTGCI
                     LEYNTQYLLKGVAGVSANVLFGGTVVGTLKDTSGTSDISSATTFVRRKAVLPDNGNGK
                     ISFVSSVTNNTTGFADWRIDNVTVRCNTQASTTVTILDENLNTSAANWLLQNLWTWNA
                     TAGFGGTGGLVTIGGGGNAYAGTSLVTYVPSISLAGSFGCQLQMFYNHANSAAENCLA
                     LHWNSSRIWAQCGSGTGGTLRLLLTAHEDTTANTLQFRCIDANGALGGNNTCTVDQLT
                     LTCRQ"
     gene            complement(3527..4768)
                     /locus_tag="PLF85_08475"
     CDS             complement(3527..4768)
                     /locus_tag="PLF85_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19740.1"
                     /translation="MSKTALAFAVLLLPLLLTSSYAEDKPAASPASPKIGTVQRRVLI
                     FDFENHTGKIEFDYLSGSISDALADSIKKTGKFRLMLREDARVNVIADGSPAPANAVP
                     GEANSVQGTSSEPPMANAPPKVNVAVNRNDAIKRGRDAGADVVVLGKFSELNGVLLMS
                     AQAYEADTRLLKVAEEILTKSDSEMFNGINVLANKIAESMARELPMFDAAEAERRNAE
                     PNRNKIEERNWELQVLGGFPYMHGLYGTDGTVGSGKGFPVHKFSGYSLGAAFWHTSLE
                     KKIYFLPKGFRLGLTTKLTLASGTADIVAADGQMISGNTKLTTQLFAGYLMMGVPYLQ
                     RDRFVLLAELGGGATYGRVLTPSDQIFASVLPGVVVGTSAAYHFSYWSVGLSYRAQAW
                     FLSTYRAFLQHDAYFYVGLRL"
     gene            4944..7400
                     /locus_tag="PLF85_08480"
     CDS             4944..7400
                     /locus_tag="PLF85_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19741.1"
                     /translation="MQKHHSKLSIFGLLFALGCSGTSTSQLLEIAFIKADTYLSIETE
                     SLVSSGKTFVVTVTARKRNINTLDLGFTNQLSVTSSGAGTLNVGTIEPWSQGKTRITL
                     SYTTTVGVGSLDTIILQLAAAGTSITASSNKISVYNEPTLHHFDVTIPTTAQINTSFN
                     VTITAKDVNGATLTSFSTPNDSGVLITPSSASGTLTVTSPASGFVNGVLTVSALYNIA
                     VFALRVRVALQSDAAIKGESGPLNIQTAALATLANFRVMAIPIASNKIRLNWTRIPEA
                     FDVIVEEELTPGNYAQIANFSGSLHYYYHTALAAASTHSYRVTIKNNAAVTLLQGTAS
                     ATTFGTGGCVTNIAAITYTTNQSWTIAGSPYCITGSVIFDAGARLEIERGALILMSST
                     GRLTFRNAAAFLPADTGSGMTRITSVSDASPPVPHLGVVLESTASPTAYAGATDDYAN
                     GSVLRHIYYEYGAEFRSDVNLKVDDVFFRQNLNRALAAYGPTKTVVTNATFQNNTGSG
                     GGGLAGIPGVTSVSAATFEYNTSNAPQGWVHGGGLRNCNQVTNSFFIGNSSSQGGGGV
                     GSCNSVIDSFFVKNYAVTFGGGQAEGVALRNYYSENSVSGNGGAIWTYSVGANVITHS
                     YFENNATAGNGGAVFLYSNSSGLQTKGLRYNWYRGNSATGQGAAVFVDTTNTAGSAIA
                     FTSLQEYYLDNATGNSALYVRQNRDATDTDNDIATIINSVFKKAASDAESEANVAFVL
                     TGTPTPVGVSNGTINNSYFETGAADCTAAFITGATSSCTTPTPNLFKTDVQWPFCAST
                     APGQATPANCVGPSWDKPVP"
     gene            complement(7404..>7911)
                     /locus_tag="PLF85_08485"
     CDS             complement(7404..>7911)
                     /locus_tag="PLF85_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19742.1"
                     /translation="LIEKLATQEMSVQKGLTYEAVLRRPQLTEESIRLIFNDLRSGGS
                     TSLWKPETEDGRTDPDRTLSGVEGRGLPVISREEEFKIAMEVKYDGYNERESNRIERR
                     LGSEDRPIPEDFDYDQLDSIKFEARAKLKRIQPRTVGQAARISGVDPSDIDILLIVLE
                     SQRRAKAG"
BASE COUNT         1791 a         2171 c         2121 g         1828 t
ORIGIN      
        1 attgattttg tatgctactc cgtccgattt gaactgaacc cctagtacct tgtggaccca
       61 ccagcccgga atttctgatt tttcagaatg cagaaccatg gtatttacaa tagatgcctt
      121 gttccaatca atattctcag actttccctg ctcgtaagtt tgctctgaaa gctgacgatc
      181 cttgtcaaga atggtcagat agtcaccact aaaatctttc atgcctttcg cacgctcgtc
      241 aggcgtaatg ggcatcttct gcagtgcttc gacggaatgg aacagactcg ctgtgtattt
      301 ctggacttcc tgaggtccgc cctttgcgta ggcatcaaat atcatctttg tatacttgtc
      361 atcggacagt tttgtgcggc tcatatccga ttgacgcaac agggggcaga aaacccagcc
      421 ctgcccggct tctgcctgca cctcgcacca cggcccggta acgttgtaaa ttgtttccac
      481 cggaccattc ttgttcaata ggctgatgcg tgttccgagc ggcaggttcg caacaatttt
      541 gcctttctgg ctgggcgtct ctctcatatt agctccgacg atggagtcaa tcgcataaaa
      601 agaagaatct ccgctaccgg gcttcttttg gcatgccatg agtagagtga gagaaatcaa
      661 agctatccac aatattttca tgatctgcga attttaatgg tcccttgcgt ccagtaaagc
      721 aaaaaaatct gctggtgcaa tcggtcattc atgcgcgcca ttcaggcaac cgaatccggt
      781 ttgttaccta cacgcctcgt gcgtcacacg gtggcgatac tcaaacagcc gctcaagctc
      841 tcgccgcgca aaccagccgc cgaaaaaagc acccacgggc ccgaacggca gtgcatattc
      901 gatgtcatca atgaggatag attcgttttc tgagaccggc tcgacgatgt gccggtgcag
      961 ccagtagcga aacgggcctt tgaccatgcg gtcggcaaac atgcgccccg cttcatactg
     1021 cacatgttcg gcttcgattg tctgccatac ggggccgata cgcgtgcgca gaataacgcg
     1081 cgttccgact gcaagagatg taggtggctg aatgatctcg cttgtttgcc acggcggttg
     1141 caagagtgcg aatgcatcgg ttcgttcgtg aaaagcaaaa aggcgagcag cgctcgcggg
     1201 cattcttgat tttttctgaa agtgttgcgt tgtcattgtt ctctctctgg tgaggcagtc
     1261 agaaaatcgc gtaccagttc aaaggccttc gcattgacca cgaggccaag gtgtgaaccg
     1321 ctcacttcgg cgtgcgttac gttgggcgaa cgctggtcga tacatgcctg ccagtctacc
     1381 acggcatcgg tctgtgagta gatcgcgagt atttcattat gaatcggcac ggctgagcgt
     1441 ctttcgacat cgcgcgccat tgcctcgaga tcgaagcccc aactcttgta cagtttgcca
     1501 accgccgtga atttgggacc tccgactacc ggcgaaccca atgtcaccac gcgcgccacc
     1561 tgtcctggta gttcgcgcgc tacctcacgc gcaacataac caccgaggct ccagccgaca
     1621 agatgcacgg gccggcccgc ttcggcgagc tgctgcacgc gtttgagcgc ctgcgcgagc
     1681 aagtcgggta cataaccgtg gttgcgaccg agaccccagt ggtgcacctc ggcgccgatg
     1741 gttttgagat agtggtgcag cacggccatg gcaacctcgt cagtaccgaa gccgggaaaa
     1801 aggacaaccg ggtgattgaa ggtatgcttg ctgcgcgtca gtgcagggta acgcagtacg
     1861 agattcgcca cctcgattgc caccgcagct tcgcgccaca atccccagcg cgagggcggc
     1921 tgcggcgaaa gttcacgtgc ctgatcgatc gctacataac tttctttcgc tgccacacgc
     1981 tgaagctaat gctcagacgc tggttcgccc agcaatttcg cattgcatgc tcaaggggga
     2041 ttttctcagt gcacgcaatg gttgcactgt tacagagggt tcgtaccgcc gcggtcgata
     2101 ttgcgggtga acgcgtgtca gaaatcgacc ctggcctgct ggtgctgctc ggcgtgggga
     2161 atgaagacaa cgaaagcaat gcggctgcgc tcgcgaaaaa gactgcggcg ctgcgcatct
     2221 tcagcgacga agccggcaag atgaacctga gcgtcgccga tacccaaggc gaagttttgg
     2281 tggtgtcgca gttcactctc atggccgaca cacagaaggg taacaggccg tcgttcgtcg
     2341 ctgctgcaaa acccgagctg gcaattccgc tctacgaatt cttcgtcgcc gagctcggca
     2401 agatactggg caaagaaatc aagacaggcc gattcgctgc agacatgcag gtgagcctgg
     2461 tcaacgacgg gccggtgaca atctggctcg aggcgtgact actgccggca ggtgagcgtc
     2521 agctggtcga cagtgcaggt attattgccg ccgagcgccc cgttggcatc gatgcagcgg
     2581 aattgcaggg tatttgccgt cgtgtcttcg tgcgcagtca gcagtaacct gagtgtgccc
     2641 ccagttccgg aaccgcactg cgcccagatc cgactgctat tccagtggag tgccaggcag
     2701 ttctcggccg cgctattggc atgattgtag aacatctgca attggcagcc gaaactgcca
     2761 gcaaggctaa tggagggcac atacgtgaca agacttgttc ctgcgtacgc attgccaccc
     2821 ccgccaatgg tcacaaggcc cccggtacca ccgaaccccg cagtcgcatt ccaggtccag
     2881 aggttctgca gaagccaatt ggccgcagaa gtattcaggt tttcatccag gattgtcacg
     2941 gtcgtcgatg cctgtgtgtt acacctcacg gtgacgttgt cgatgcgcca gtcggcgaag
     3001 ccagtagtgt tgtttgtcac gcttgagacg aacgagattt tgccgttgcc attatcgggc
     3061 aagacagctt tcctgcgcac aaaggtcgtc gcactggaaa tatcggatgt gccactggtg
     3121 tccttcagag tgccaacaac cgtacccccg aacaagacat tggcagaaac gccggcgacg
     3181 cccttaagta gatactgtgt gttgtattcc aaaatacatc cagttcgacc ggtcaggtca
     3241 ataaaggcag ccgtcgtaat gctaaaagtt gcggcggtcg tgctcgaagg actcagcagt
     3301 gctaaactgc cggttcccga tgcgactgtc gtccagcttc cggaagactg gttccatttc
     3361 gccagcccat tatcaaaatc ttcgctgaaa atcagattcg ggtcgggcag tacctccggg
     3421 ggcggggtgt tgcgccgaat aaaatcttcc atctgtgcct ctgccgtgca agcggtgagc
     3481 cccgcagcaa taaggatgta accgacaata cgcatcatgt ctcctcctaa agtctcaatc
     3541 ccacataaaa ataagcgtcg tgctgcaaga acgccctgta cgtcgataag aaccatgcct
     3601 gcgcgcggta gctgagtccc acgctccagt aggaaaagtg ataggctgcg ctcgtgccaa
     3661 cgactacgcc cggcaagaca ctcgcgaaaa tctggtcgct tggcgtcagc acgcgaccgt
     3721 aggtggctcc accgccgagt tcggccaaga gcacaaaacg gtcgcgctgc agatagggaa
     3781 ctcccatcat caagtagccg gcgaagagtt gtgtggtaag tttagtatta cctgagatca
     3841 tctgcccgtc tgccgcaacg atgtcggcag tgcccgatgc gagcgttaat ttggtcgtga
     3901 gccccaggcg aaaacctttc ggcaagaagt agattttttt ctcgaggctg gtatgccaaa
     3961 aagcggcgcc gagactataa ccagagaatt tgtgaacggg aaaacctttg ccgctgccca
     4021 ctgtgccgtc ggtgccgtaa agaccgtgca tgtagggaaa gccgccgaga acctgaagct
     4081 cccaattgcg ctcttctatc ttatttcggt ttggttcggc attgcgtctt tcggcctcag
     4141 cggcatcgaa catcggcagt tcgcgcgcca tagattcggc gatcttgttc gcgagcacgt
     4201 tgatgccgtt gaacatctcg ctgtctgact tggtgagtat ttcttcagca accttgagca
     4261 gccgggtatc tgcttcatag gcctgcgcgc tcatcagcaa gaccccattc agttcgctga
     4321 acttgcccag caccacgaca tcggcgccgg catcgcggcc acgtttgatt gcatcgttac
     4381 gattcaccgc tacattcacc ttgggtgggg cgtttgccat aggcggttcg ctgctcgtgc
     4441 cctgaacaga atttgcttca ccaggcacgg cattggcagg tgccggtgac ccgtctgcga
     4501 tgacattcac ccgcgcatcc tcgcgcagca tcagcctgaa tttaccggtt tttttgatcg
     4561 agtcggccag cgcgtctgag atgctgccag agagatagtc gaattcaatc ttgccggtat
     4621 ggttctcgaa atcgaagatc agcacccggc gctgcacggt accgattttt ggcgatgcgg
     4681 ggctggccgc aggtttatct tcagcatatg aggaggtcaa tagcaagggc agaagaagca
     4741 cagcgaatgc aagggcagtt ttcgacacgg tggggggtat aacggtgggg ggtatatcgg
     4801 caagaaaaaa ttgcccgtca attctttgca ggtgtaacta acccccctat tcagggggct
     4861 agcgcctttc ttaatattct cgacgctcga tgtattctgt gtgtatctca ctgctcaggg
     4921 ctgccgcagg gcgtgaaata cgtatgcaga aacatcattc aaaactgagt atatttggac
     4981 ttctgtttgc tctcggttgc tccggcacat caacctcgca gctgctggaa attgccttca
     5041 tcaaagctga tacctatttg tccatcgaaa cagaatcgct cgtgagttcg gggaagactt
     5101 tcgtggtcac ggtaacagcc cgcaagagga acatcaacac cctcgacctt ggctttacca
     5161 accagctttc cgtaacatcg agtggtgccg gcacgctgaa tgtcggtacc atagagccat
     5221 ggtcgcaggg caaaacgcgc atcacgcttt cttatacgac aacggtcggt gtcggtagcc
     5281 ttgatacgat cattctgcag ctcgctgccg ccggcacatc gatcactgct tcgtcgaaca
     5341 agatatctgt gtacaacgag ccgacgttgc accattttga cgtgacgata ccgacaacag
     5401 cgcaaataaa cacctcgttc aacgtcacga tcacagcaaa ggatgtaaac ggcgccacgc
     5461 tgacctcgtt cagcactccc aacgattcgg gtgtgttgat taccccatcg agcgcatcgg
     5521 gcactctgac ggtcacttcg cctgcttcgg gatttgtcaa tggggtcctc actgtctcgg
     5581 ctttgtataa catagcagtc tttgccctca gagtaagggt tgcgctgcag tcagatgcag
     5641 ctatcaaagg cgagtctggc ccgttgaaca tacaaacagc ggcgctggcc acgcttgcga
     5701 actttcgcgt gatggcgata cccattgcca gcaacaagat acgtctaaac tggacaagaa
     5761 ttcctgaggc atttgatgtc atcgtggagg aagagttgac gcccggcaac tatgcccaga
     5821 tagccaattt ttcgggtagc ctgcattatt attaccacac agcgctcgcg gcagccagta
     5881 cgcattctta cagggtgacg ataaaaaata acgcggccgt cactctttta cagggtacag
     5941 caagtgcaac gacctttggt acgggcgggt gcgtcacgaa tatcgcagct attacttaca
     6001 ccacgaacca gagctggacc atcgcaggct cgccttattg catcacgggt tcggtaatct
     6061 ttgatgccgg tgcgcgactg gaaatcgaac gcggtgcact gatcttgatg tcgagtaccg
     6121 gacgcctaac atttcgcaac gccgctgcgt ttttgcctgc cgacactggc agcggcatga
     6181 cgcgcattac ttcagtcagt gacgcaagtc cgccagttcc acaccttggc gtagtactgg
     6241 agtcgacggc gagccccaca gcgtacgctg gtgcgactga tgattacgcc aatggcagcg
     6301 tgttgcgcca catctactat gagtatggcg cagaatttag gagcgatgtc aatttaaagg
     6361 tcgatgacgt cttcttcaga caaaatttaa acagggcgct ggcagcgtac gggcctacca
     6421 aaacagtcgt tacgaatgcc acgtttcaaa acaacacggg aagcggtggt ggcggcctgg
     6481 caggcattcc gggtgtaacc agcgttagcg cagctacatt cgagtataac acgtcgaatg
     6541 ccccccaagg ctgggtccat ggtggtggtc tacgaaactg taatcaagta accaatagtt
     6601 tcttcatcgg aaatagttct tcacaagggg gaggcggcgt cggttcgtgt aactctgtta
     6661 ttgattcctt cttcgtaaaa aattacgctg tcaccttcgg tggtgggcag gccgaaggtg
     6721 tcgcgctgag aaattattat tctgaaaaca gcgtatcggg caatgggggc gcgatttgga
     6781 catatagtgt tggcgcgaat gtcatcactc acagttactt cgaaaacaac gcgacggcag
     6841 gtaatggtgg tgcggtcttc ttgtattcaa acagctcagg tctgcaaacc aagggccttc
     6901 gctataattg gtaccgtgga aactcggcca caggacaagg tgccgcagtc ttcgtcgata
     6961 caactaacac agcaggttcc gcgattgcgt tcacctcact tcaagaatac tatctggaca
     7021 atgcgaccgg caattcggcc ctatatgttc gccaaaaccg agatgcaacc gataccgata
     7081 atgatattgc caccattatc aatagtgtct ttaagaaagc cgcttcggat gctgaatcgg
     7141 aggcaaatgt cgcattcgtt ttgacaggta caccgactcc ggtcggcgtt tcgaacggaa
     7201 ccatcaacaa cagctacttt gaaaccgggg cggcagattg cacggccgca ttcatcactg
     7261 gagcaaccag ctcatgcaca acgccaaccc cgaatctgtt caagacagat gtgcagtggc
     7321 cgttttgtgc cagcactgcg ccaggccagg cgacgccggc aaattgcgtc gggccgagtt
     7381 gggataagcc agtgccgtga tggtcatcct gccttcgcgc gccgctggct ttcgagcaca
     7441 atgagcagaa tgtcaatatc acttggatca acccctgaga tacgcgcggc ctgccccaca
     7501 gtgcgtggct gaatcctctt taatttagcc cgcgcctcaa acttgatgct gtcgagctgg
     7561 tcgtagtcaa aatcttcggg tatcggccgg tcttcactgc cgaggcggcg ctcgatgcgg
     7621 ttcgattcgc gctcgttgta gccgtcgtat ttgacttcca tcgcgatctt gaattcttct
     7681 tctcgagaga tgactggtaa tccgcgccct tcgactccgc tcagggtgcg gtcggggtcg
     7741 gtgcgaccgt cttcggtctc tggcttccag agcgaagtcg aaccgcccga gcgcaaatca
     7801 ttaaaaatca gtcggataga ctcttcagta agctgcggtc gccgtagcac ggcctcatac
     7861 gtgaggccct tctgcacgct catttcctgc gtcgcaagtt tttctataag a
//
LOCUS       DAOQJF010000135         7893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_4988_length_7893_cov_5.651569, whole genome shotgun sequence.
ACCESSION   DAOQJF010000135 DAOQJF010000000
VERSION     DAOQJF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4988_length_7893_cov_5.651569"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..1625
                     /gene="tuf"
                     /locus_tag="PLF85_08490"
     CDS             420..1625
                     /gene="tuf"
                     /locus_tag="PLF85_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNE19743.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTLTAAITTVLARKFGGKN
                     KAVSYAEIDNAPEERARGITIATSHQEYETANRHYAHVDCPGHADYVKNMITGAAQMD
                     AAILVVSAVDGPMPQTREHILLARQVGVPQIVVFLNKIDMLPESDRAEMQELVEMEVR
                     DLLSKYGFPGGEIPFVAGSALKAVEGDAGDLGEGAIVKLAETLDAYVKDPVRVVDKPF
                     LMPVEDVFSITGRGTVCTGRVERGQINVNEEIEIVGIRDTQKTIVTGIEMFRKLLDFG
                     QAGDNVGLLLRGTKKEDVERGQVIAKPGSVKPHKKFSGEVLVLTKDEGGRHTPFFDKY
                     RPQFYFRTTDVTGTVNLPTGVEMVNPGDNVTITAELISPIAMEEGLRFAIREGGRTIG
                     AGVVGKIIE"
     gene            1632..1940
                     /gene="rpsJ"
                     /locus_tag="PLF85_08495"
     CDS             1632..1940
                     /gene="rpsJ"
                     /locus_tag="PLF85_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNE19744.1"
                     /translation="MAAQKIRVKLKSFDQRLLDQSTGEIVGTVQRTRAKIAGPVPLPT
                     KVEKFTVLRSTFVYKDSRDQYEMRTHSRLIDILEPTEDTIDALMKLQLPAGVMVYIKS
                     "
     gene            1975..2601
                     /gene="rplC"
                     /locus_tag="PLF85_08500"
     CDS             1975..2601
                     /gene="rplC"
                     /locus_tag="PLF85_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNE19745.1"
                     /translation="MVKGLLAKKIGMLSVWSDAGEQVAVTAVQAGPCPVLQVKTKEKD
                     GYTAVQIGFDEIPERLANKAALGHQKIAKEKTGKVVRKAIELRDFDTDKQPGDSLTCE
                     VFAPGEKILVTGTSKGKGFAGVVKRYNFGGGRMTHGSKFHRAPGSIGNRKIPGEVQKG
                     KRMPGHKGAKNVTVKNVEVFRVLPEQNVVLLKGAIPGVKGATVFLYQN"
     gene            2611..3306
                     /gene="rplD"
                     /locus_tag="PLF85_08505"
     CDS             2611..3306
                     /gene="rplD"
                     /locus_tag="PLF85_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNE19746.1"
                     /translation="MASLKVYSNTGSASGEASISDKIASLKINKHLIWEVIKAEQANA
                     RQGTHKTKTKGEVSGGGKKPWKQKGTGNARQGSIRSPQWRHGGIVFGPQPRSYAESIS
                     RQKKQIGVAHILAAKVQANKVAIYDGLNLEKPSSKTAFQAISKITEGAKLQTTGREKK
                     KLRANSNKARHSIAVIMSPAEVKDTKSLRNIPWVKPLSSSRIAATSLFYNEGLVISKA
                     ALQELDKKFSALL"
     gene            3314..3598
                     /gene="rplW"
                     /locus_tag="PLF85_08510"
     CDS             3314..3598
                     /gene="rplW"
                     /locus_tag="PLF85_08510"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNE19747.1"
                     /translation="MNLYDVIKKPLVSEKAEALRAQNCYVFEIDMKANKTLVKQAIRK
                     IFGVTPIKVNTLVARADGKANRYNIGYTNRRKKAYVYLGKNDKIALFEGV"
     gene            3607..4449
                     /gene="rplB"
                     /locus_tag="PLF85_08515"
     CDS             3607..4449
                     /gene="rplB"
                     /locus_tag="PLF85_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNE19748.1"
                     /translation="MAIKRFNPNTPTLRYKTVLKSSEVTATEPYKPLLTYLKETAGRN
                     NHGRITVRRRGGGHKRRYRIIDFRRNKMNVPAIVETVEYDPNRSANIALVCYADGARA
                     YIIAPQGLQVGQKIISGEKVEIEVGNAAPIGTLPIGANVHNIELTLGRGGQLARSAGS
                     FAVITGRDGEYTIVKLPSGEARKIHSRCMATIGIVGNHEHELVSIGKAGRSRWLGKRP
                     RVRGVAMNPVDHPLGGGEGKTSGGRHPVTPWGKPTRGKKTRNKRKTSERFIVSRKVRT
                     RGGE"
     gene            4449..4736
                     /gene="rpsS"
                     /locus_tag="PLF85_08520"
     CDS             4449..4736
                     /gene="rpsS"
                     /locus_tag="PLF85_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNE19749.1"
                     /translation="MSRSLKKGPFIDQHLFKKVEKAKATNDKKPIKTWSRRSTIFPEM
                     IGMTFLVHNGSKFIPVFVNENIVGHKLGEFAPTRTFKGHTSLDKKKSAPGG"
     gene            4748..5086
                     /gene="rplV"
                     /locus_tag="PLF85_08525"
     CDS             4748..5086
                     /gene="rplV"
                     /locus_tag="PLF85_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNE19750.1"
                     /translation="MESKASVDQVKMSARKMRLVANEIRGYEYPEAVDILKHIPRKGS
                     RIILKALQSAAANARVLNPSVDERSLFIKKIFVDGGPLMKRFMPRARGRADRIQKKTS
                     KLTIVLSDEG"
     gene            5088..5765
                     /gene="rpsC"
                     /locus_tag="PLF85_08530"
     CDS             5088..5765
                     /gene="rpsC"
                     /locus_tag="PLF85_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNE19751.1"
                     /translation="MGQKTNPIGLRLKITRTWDSVWYHKKETYAQILHEDLKIRKHIK
                     DRFNKSGIVKVGIERFPEKVHIKLHSTKPGLVIGQQGKKIEALKASLRTMVTKPLEVK
                     IIEVAKPDGSAQALAESVALQLEDRMAFRRAMKMALRSAIRAGALGVRVMVSGRLNGA
                     DMARREQYLEGRVPLHTLRAIIDYGTAEADTTFGKIGVKVWIYNGDYLEAKESDDDKY
                     TIKRREA"
     gene            5773..6183
                     /gene="rplP"
                     /locus_tag="PLF85_08535"
     CDS             5773..6183
                     /gene="rplP"
                     /locus_tag="PLF85_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNE19752.1"
                     /translation="MLAPKRLKWRKPHTLNLKGFSKGGNTVAFGDLGLKAVSGERITA
                     RQIEAARVAMMRHVRRGAKVWIRIFPDRPVTKKPAETRQGKGKGSVEYYVAIVKPGKV
                     MFEMGGADEKVMAEALTRAASKLPIKTKIVKRAK"
     gene            6233..6463
                     /gene="rpmC"
                     /locus_tag="PLF85_08540"
     CDS             6233..6463
                     /gene="rpmC"
                     /locus_tag="PLF85_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNE19753.1"
                     /translation="MAKADKKKTGKKVNFAELSDAELSRYHLDFRKELQEIRFSLVTS
                     SYTNNARIGILKKDIARILTVQKQRKTQAAKA"
     gene            6460..6750
                     /gene="rpsQ"
                     /locus_tag="PLF85_08545"
     CDS             6460..6750
                     /gene="rpsQ"
                     /locus_tag="PLF85_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNE19754.1"
                     /translation="MSEVKKIRKTVQGTVVSAQKIKDGKTIVVKVSFRKQDPRFKKTI
                     GMSKKVMAHDEKEEARQGDVVVIEESRPYSKRKRYVLQKIVTRRAEEAPVAV"
     gene            6769..7161
                     /gene="rplN"
                     /locus_tag="PLF85_08550"
     CDS             6769..7161
                     /gene="rplN"
                     /locus_tag="PLF85_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNE19755.1"
                     /translation="MLQMLSWLKVADNTGARQVQVVKVLGGSKKRYAGVGDVIVVAVK
                     SATPAYGLKDSSGKKDHKKQVQRAVIVRTKKELRRPDGSYIRFDENACALIDNQNNPK
                     GTRIFGPVARELRDRQFMKIISLAPEVL"
     gene            7168..7512
                     /gene="rplX"
                     /locus_tag="PLF85_08555"
     CDS             7168..7512
                     /gene="rplX"
                     /locus_tag="PLF85_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNE19756.1"
                     /translation="MKAAPEKIKTRLKVKDEVIVIAGKHKKSRGEILRIDREKKQVVI
                     KGVNLRKRFARPTQENPKGGVIEIEMPIALSNVQYYDSKEKKPSRVAFGLDKGEKKTR
                     YSVASGRKRTLD"
     gene            7515..7817
                     /gene="rplE"
                     /locus_tag="PLF85_08560"
     CDS             7515..7817
                     /gene="rplE"
                     /locus_tag="PLF85_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNE19757.1"
                     /translation="MAAATQSNPARLRRLYSEKIRPELKQELGVSSVMQVPVLKKIVL
                     NVGAGFAVANPKSLDGVVAEIAAITGQAPVKTRAKEAISNFKLREGLPIGVSVTLR"
BASE COUNT         2180 a         1852 c         2172 g         1689 t
ORIGIN      
        1 gtagggagat tggcatgccc ttaaatacag ttttccgggc aagccttccg tccgatatgt
       61 cgacgcaagg tttcataacg acgagccggg caatttcttt ttgcagaatt acggcgcgga
      121 tcgtacagat gcgacataac ataggcagag gtccgggaat tcgatcatag cggccgggta
      181 tttgaccggt aaccggaaaa ccctaatttt attgtcagcc tgacacaatt tcaaaaactg
      241 actgcctgta cttggtggtt gaacccttcg gagcgatacc gatcggccga cctggattac
      301 aaaaccagtg cgaactttta tgatttgagg ctttgtcctg cagacggccc atgcgaatct
      361 ttcctgattc gccgagggtg cagtgtgggg cagagcccga aatattgagg agaacgtaaa
      421 tggcaaagga aaaatttgac agaagcaaac cacacgtaaa cgtcggcaca attggccacg
      481 ttgaccacgg caaaaccacg ctgacagcgg cgatcacgac agtgctcgca cgtaaattcg
      541 gcggaaaaaa caaagcggta tcatacgctg aaatcgataa cgcacctgaa gagcgcgcac
      601 gcggtattac tatcgctacg tcgcaccagg aatacgaaac agcaaaccgt cactatgcgc
      661 acgttgactg cccgggtcac gctgactatg tgaagaacat gatcaccggt gcggcgcaga
      721 tggatgcagc gattctcgtt gtttcagctg ttgacggccc tatgccacag actcgtgagc
      781 acatcctgct cgctcgtcag gtcggcgtgc ctcagatcgt tgttttcctg aacaaaatcg
      841 acatgctgcc agaaagcgac cgtgcagaaa tgcaggagct cgttgaaatg gaagttcgcg
      901 atctgctcag caaatacggc ttcccaggcg gagaaatccc attcgttgct ggctcagccc
      961 tgaaggctgt tgaaggcgac gcaggagacc tcggtgaagg cgcaattgta aagctcgctg
     1021 aaacactcga tgcatacgtc aaagatcctg tgcgcgttgt agacaagccg ttcctgatgc
     1081 cagtagaaga cgtgttctcg atcactggcc gtggtactgt atgtactggt cgtgttgagc
     1141 gcggtcagat caacgtgaac gaagaaatcg aaattgttgg tatccgtgat acacagaaaa
     1201 ccatcgttac cggtatcgaa atgttccgca agctgctcga cttcggtcag gcgggggaca
     1261 acgttggtct gctgctccgc ggtaccaaga aagaagacgt tgagcgtggt caggtaatcg
     1321 ccaagccagg ttcagtaaaa cctcacaaga agttttctgg tgaagttctt gttctgacta
     1381 aggatgaggg cggtcgccat actccattct ttgataagta tcgtccacag ttctacttca
     1441 gaactacaga cgtaactggt actgttaacc tcccaacagg tgttgaaatg gttaaccctg
     1501 gtgataacgt aacgatcact gctgagctca tcagcccgat cgctatggaa gaaggtcttc
     1561 gcttcgctat ccgtgaaggt ggtcgtacca tcggcgcggg tgttgtcggc aagatcatcg
     1621 agtaacagaa aatggccgct cagaagatac gcgtaaaact caagtcgttc gaccagcggc
     1681 tgctcgacca gtcgaccggt gaaatcgttg gcacagtgca gcgtacccgc gcaaaaatcg
     1741 cgggtccggt gcctctgccg acgaaggtcg aaaagttcac ggttctgcgt tcgacattcg
     1801 tctacaaaga ttcacgtgat cagtacgaga tgagaacgca ctcacggctc attgacattc
     1861 ttgagccaac agaagatacc atcgatgctc tcatgaagct acagcttccg gcgggtgtga
     1921 tggtttacat taagtcttga ttcagattta ttagggacac caagggattt caacatggta
     1981 aaaggcctgc ttgcaaagaa aatagggatg ctgagcgtct ggagtgatgc cggggaacag
     2041 gtagctgtta ccgcggtaca agccggccct tgtccggttc ttcaggtgaa gactaaggaa
     2101 aaagacggct acacagccgt acagatcggc ttcgacgaga ttcctgagcg cctcgccaat
     2161 aaggcggctc ttggtcacca gaagatagct aaggaaaaga ccggcaaggt cgttcgcaag
     2221 gcaatcgagc tgcgtgactt cgacacggac aagcagccag gagattctct gacgtgcgaa
     2281 gttttcgctc ccggagagaa gattctggta actggaacca gtaaaggtaa aggttttgct
     2341 ggcgtcgtga agcgttacaa tttcggcggt ggtcgtatga ctcacggttc aaaatttcac
     2401 cgtgcgccgg gttcgattgg taaccgcaag attcctggtg aagttcagaa aggcaagcgg
     2461 atgccaggtc acaagggcgc taaaaacgta acagtgaaga acgttgaagt gtttcgcgtg
     2521 ctgcctgagc agaacgtggt gctgctgaaa ggtgcaattc cgggcgttaa aggcgcaacg
     2581 gtatttctct accagaatta aggaaatcgt atggcatcgt taaaagtata ttcaaacacc
     2641 ggctccgctt caggcgaagc gtcaatctca gacaagattg catctctcaa gatcaataaa
     2701 cacctgatct gggaagtgat caaagctgag caggcgaatg cccgtcaggg aactcacaag
     2761 acgaaaacga aaggtgaggt atcgggtggt ggcaaaaagc cgtggaagca gaaaggcact
     2821 ggtaatgctc ggcaggggtc aatccgctca ccacagtggc gccatggtgg tattgttttc
     2881 ggtcctcagc cacgcagcta tgcagaaagc atttcgcgcc aaaagaagca gattggtgtt
     2941 gctcacatac tggcagcgaa ggtccaggcg aacaaagtgg ccatttatga tggtctgaat
     3001 ctcgagaaac catcttcaaa gaccgccttc caggcgatca gcaagattac cgaaggtgca
     3061 aagttgcaga ctacaggccg cgagaaaaag aagctccgtg ctaacagcaa caaagcgcgt
     3121 cactcgattg cagtgatcat gtcgcccgca gaagtgaagg acacaaagtc gctgaggaat
     3181 attccctggg tgaagccgct gagttcttca cgtatcgccg cgacctcgct tttttacaac
     3241 gaaggcctcg ttatcagcaa ggcagccctg caggaactcg ataagaagtt ctctgcgcta
     3301 ctctagagga aaaatgaacc tgtacgatgt aattaaaaaa ccgctggtct cagagaaagc
     3361 agaggcgctg cgcgcccaga actgctatgt tttcgagatc gacatgaagg ctaacaagac
     3421 gctggtgaaa caggcgatcc gcaagatctt cggcgttaca cccataaagg tgaataccct
     3481 tgtggctcgg gcagacggca aagctaatcg ctacaatatc ggttacacaa accgccgcaa
     3541 gaaggcgtat gtgtacctgg gcaagaacga taaaatcgcc ctttttgaag gagtataaga
     3601 ggtaatatgg caatcaaacg ttttaatccc aatactccaa cactccgtta caaaacggtt
     3661 ctgaagtcct cggaagtaac ggcaacggag ccgtacaagc cgctgttaac ttatctaaaa
     3721 gagacagctg gccggaacaa tcacggtcgc attacagtgc gtcgtcgcgg cggtggccat
     3781 aagcgccgct accggatcat tgacttccgc cgcaataaaa tgaatgtgcc ggcaattgtt
     3841 gagacagttg aatacgatcc gaatcgtagc gcgaacatag cccttgtatg ttacgctgac
     3901 ggcgcgcgtg cttatatcat cgcgccacag ggtttgcagg taggccagaa aatcatctcc
     3961 ggtgaaaaag tggagattga agtaggtaac gctgccccca tcggaacgct tcccatcggt
     4021 gcaaacgtgc acaatatcga actcacgctc ggtcgtggtg gtcagctcgc tcgttcggct
     4081 gggtcgttcg cggttattac gggccgtgat ggtgaatata ctatcgtaaa acttccgtca
     4141 ggcgaggcaa gaaagattca cagccgctgc atggcgacta tcggtatcgt tggaaaccac
     4201 gagcacgagc ttgtgtcgat tggtaaggct ggtcgttcac gctggctcgg aaagcggccg
     4261 cgcgtgcgtg gtgtggcgat gaacccggtt gaccacccgc ttggtggtgg tgaaggtaaa
     4321 acttccggtg gtcgtcaccc tgtgacaccc tggggtaagc ctactcgcgg taagaaaacg
     4381 cgcaataagc gtaagaccag cgagcgtttt atagtatcac gcaaagtacg taccaggggt
     4441 ggtgaataat gtcacgttct ctcaagaaag gtcctttcat agaccagcac ctttttaaaa
     4501 aggtggaaaa ggccaaggca acaaatgata agaagcctat caagacttgg tcacgtcgct
     4561 caacgatatt ccctgagatg atcggtatga cttttctggt ccataacggc agcaagttca
     4621 tcccggtgtt cgtaaatgag aacattgttg gccataagct gggcgaattt gccccgacgc
     4681 gtacgttcaa gggtcataca tctcttgata aaaagaaatc cgccccgggc ggctaacagg
     4741 atagtccatg gaatcaaaag catctgttga tcaggtcaaa atgtcagccc gcaagatgcg
     4801 tcttgtggca aacgaaatca ggggttatga ataccccgag gccgtggata ttctgaagca
     4861 tatcccgcgt aaaggctcac gcatcatact gaaggcgcta cagagcgctg cggcgaatgc
     4921 cagggttctg aatccttctg tggatgagcg ttcgctcttc atcaagaaga ttttcgtgga
     4981 tggtggcccc ttgatgaagc ggttcatgcc acgggcacgg ggacgcgcag accgtatcca
     5041 gaagaagaca tcaaaactca cgatcgttct ttcagacgaa ggttaaaatg ggacaaaaaa
     5101 caaatccgat tgggctccgc ctcaaaatca cgcgcacctg ggatagtgtc tggtatcata
     5161 aaaaagaaac ctacgcgcaa atcctgcacg aagatctcaa aatccgtaaa cacataaagg
     5221 accgctttaa taagagcggc atcgttaagg tcggcattga gcgttttcct gaaaaggttc
     5281 atatcaagct ccatagtacg aaacccggtt tggtgattgg ccagcagggc aaaaagatcg
     5341 aggccctgaa ggcgtcgctc cgcacaatgg tcacaaagcc gctcgaagta aagataattg
     5401 aagtggccaa acccgatggt tcggcacagg ctcttgctga atcagtggcg ttgcagcttg
     5461 aagaccgtat ggcattccgc cgtgccatga agatggcgct gcgttctgcg atacgtgccg
     5521 gtgctctggg tgtccgtgtt atggtttctg gtcgtctgaa cggtgccgac atggctcgcc
     5581 gggagcagta tctcgaagga agggtgcccc tgcacaccct gcgcgcaatt atcgactatg
     5641 gtaccgcaga agcggacacg acgttcggta agatcggcgt gaaagtatgg atttataacg
     5701 gcgactatct tgaggcaaag gagagcgacg atgataaata cacaatcaag cgtcgcgaag
     5761 cgtaggtaaa acatgctggc accgaaaaga ctcaaatggc gtaagccaca cactctcaat
     5821 ctgaaggggt tctcgaaagg cggaaatacc gtggctttcg gcgacctcgg cctcaaggct
     5881 gtctcaggtg aacgtatcac cgccaggcag attgaagcag cccgcgtggc catgatgcgc
     5941 cacgtgcgcc gtggcgccaa ggtatggatc cggatatttc ctgaccgtcc cgtgacaaag
     6001 aagccggccg aaacccgcca gggaaaaggc aagggtagcg tggaatacta cgttgcgatc
     6061 gttaagccgg gcaaggtgat gtttgaaatg ggtggggcag acgagaaagt aatggcagaa
     6121 gcgctgacgc gtgcggcctc taagctgccc atcaagacga agatcgtcaa gcgggcgaag
     6181 taaatgatcg tgtttgacgg catgtttcaa aataaaaaaa tggacttctc ccatggctaa
     6241 agcggataag aaaaaaacag gaaaaaaggt caatttcgcg gaactgtccg atgcagagct
     6301 ctcgcggtac cacctggact tccgtaaaga actccaggag attcggttca gcctggtgac
     6361 ttcttcgtat acgaacaatg cccgcattgg cattctcaag aaggatattg cgcggattct
     6421 gaccgtacag aaacaaagaa aaacgcaggc ggctaaggca tgagcgaagt aaagaagatt
     6481 cggaaaacgg ttcagggaac cgtagtttcg gcacagaaga ttaaagacgg caagactatt
     6541 gttgttaagg tatcatttcg taaacaggat ccacggttta agaagaccat tggaatgagc
     6601 aagaaagtca tggcgcatga cgagaaagag gaagcgcgcc agggcgatgt cgttgtgatc
     6661 gaagagtcac gcccgtattc aaagcgcaag cgctacgtgc tgcaaaagat cgtcactcgc
     6721 cgggcagaag aagctccggt agctgtataa ggataataag aggctaatat gctgcaaatg
     6781 ctttcatggc tcaaagtagc cgataacaca ggtgcccgtc aggttcaagt cgttaaagtt
     6841 ctgggcggct caaagaagcg ttatgccgga gtaggggatg tcatcgttgt tgcggtgaaa
     6901 tcagcaactc cagcttatgg cttgaaggat tcatcgggaa agaaggatca taagaaacag
     6961 gtgcagcgtg cggttatcgt tcgtaccaag aaagagctgc gccgccctga cggttcttat
     7021 atccgctttg atgaaaatgc atgcgctctg attgataacc agaataaccc gaaggggacg
     7081 cgtattttcg gaccagttgc ccgtgagttg cgcgatcgcc agttcatgaa aatcatctcg
     7141 ctggcgccgg aagtattgtg aggtaaaatg aaagcagctc cggaaaaaat taaaacccgc
     7201 ctcaaagtca aagatgaagt gattgttatt gctggcaagc acaagaaatc gcgtggagaa
     7261 atactgcgga ttgatcgtga gaagaagcaa gtggttatca agggtgtgaa tctccgtaag
     7321 aggttcgcac gcccgacaca ggaaaaccct aagggtggcg tgatagagat cgaaatgccg
     7381 attgcgcttt cgaacgtaca gtattatgat tctaaggaaa agaagccgag tcgtgtagct
     7441 tttggtctgg ataaaggcga aaagaagaca cgctactcgg ttgcatcagg gcgcaagagg
     7501 accctagact gattatggca gcagcaacac aatctaatcc cgcacggctt cgcagacttt
     7561 acagcgaaaa gattcggccg gaacttaagc aagagctggg cgtgagctcg gtaatgcagg
     7621 tgccggttct gaaaaagatc gtcttgaacg ttggggcagg attcgcggta gcgaacccga
     7681 aatccctgga tggggtagtt gcggaaattg cagcgattac cggacaggct cccgtaaaga
     7741 cgcgggcaaa agaagctatt tctaacttca aattgcgcga aggtttgccg atcggcgtga
     7801 gcgttacgct tcggtagtaa cttccatacg cttctcagga acaatcgtaa ccataaaagg
     7861 ctttgccttt ttggctacag agataacatc atc
//
LOCUS       DAOQJF010000136         7819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5070_length_7819_cov_5.010948, whole genome shotgun sequence.
ACCESSION   DAOQJF010000136 DAOQJF010000000
VERSION     DAOQJF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5070_length_7819_cov_5.010948"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1587)
                     /locus_tag="PLF85_08565"
     CDS             complement(76..1587)
                     /locus_tag="PLF85_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNE19758.1"
                     /translation="MVNLTGVLGIIFVGISCLLLSRNRGAIDWRTVLWGLVLQISFAL
                     LIIRGDSIAELFSFFPLTQSVFLAIVACQVLALWLVTRYAPTLAARLPMVWVRRVVWA
                     EAIFYLLRFNVVGAIFESLKTGAAQVLKFSGVGASFVFGVLGSEEAMRASFTTALGDK
                     AAGIAFVFAFQVLPTIIFVASIFSVLYYLNVMQPLIRAIAKFINRFMRASGAETLDVA
                     ANIFMGQTEAPLTIKPYLAGLTRSELFTVMVSGMAHCSAGILIVYVAVAGVDAKHLLA
                     SVIMTAPGSIMLAKMVFPETGLPETAQGGAAGSQQAAPPPVDENRPVNFIDAAARGAS
                     EGLLLAANVAAMLIAFIALIALVNGIWAAAREAALAQTSGALHGFFAAIPATIQQVLG
                     LLFAPVAFVAGVPWSEAVQVGNLLGTRIVLNEFVGYIELGKIREVLSPKAFLISTYML
                     CGFGSLTSIAIQAGGIGALVPQRRAELASLGFLAVVVGVLSNILAASIAGILF"
     gene            complement(1590..2021)
                     /locus_tag="PLF85_08570"
     CDS             complement(1590..2021)
                     /locus_tag="PLF85_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19759.1"
                     /translation="MKPARVPPHFYIVHLALMLAPAIFAAVVYFLIQSGNAAAAVPGE
                     QSVVFQSVAAAMAVIAVGFSQLLPRFMLRDEKYVPLQKYFTMKIVQWAMLEGAALFIS
                     VIFFLSHHMNLLIPIGVLIALIAVLRPTNEELERHNVKVEG"
     gene            2125..3372
                     /gene="icd"
                     /locus_tag="PLF85_08575"
     CDS             2125..3372
                     /gene="icd"
                     /locus_tag="PLF85_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNE19760.1"
                     /translation="MAYEKIRLPETGEKIAWNGKLQVPDQPIIPYIEGDGTGPDIWKA
                     SVRVLDAAVAKAYGQKRKISWLEVFAGEKSNNLYGANTWLPEETLECIAEHKVAIKGP
                     MSTPVGGGIRSVNVALRQKLDLYACVRPVQYFDGVPSPVRRPEDVDMVIFRENTEDIY
                     AGIEYEPGSEGAKKIARVLEETGLIKNVRFPETVSYGIKPVSREGSERIVRAAIEYAI
                     ANKRKSVTLVHKGNIMKYTEGYFCKWGYETARKYFAAETVAWDECGGNPPAGKILIKD
                     SIADAFLQQVLLRPKEYDVIATLNLNGDYLSDALAAQVGGIGIAPGANINYLTGSAVF
                     EATHGTAPKYAGLDKVNPSSVILSGEMMLRYLGWTEAADCIISALKKTIAQKTVTYDF
                     ARMMEGAKEIKCSEFADAIIANL"
     gene            complement(3395..4216)
                     /locus_tag="PLF85_08580"
     CDS             complement(3395..4216)
                     /locus_tag="PLF85_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE19761.1"
                     /translation="MTEKPVLTRVIESTGGNILQITMNRPEVHNACNGEMAKYLLEAW
                     LRFRDDDSLAVAVLHGAGDKSFCAGADLTALDTLAGGLLNSTSPDAAALERYAQNDPG
                     PMGGSRIVQQKPVITVSHGYTYAGGLELFCHGHIRIAEPQALFSVACRRWGVPLVDGG
                     TVYLPRLLNWGAALPLIITGQRIRAQRAYEIGLVWELVRKGKGLERALRLAAQICAQP
                     RDALVADLSSALNGSHLPLKEALTLEARNLYPVATSKSMKDGVSRFNEGERFWVV"
     gene            4289..5431
                     /locus_tag="PLF85_08585"
     CDS             4289..5431
                     /locus_tag="PLF85_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNE19762.1"
                     /translation="MSALTILSDEEQSYFDAIKDFAKKEVAPHVTAMDEAQKINPDIV
                     KKLFEMGLMGIEVPQELEGSGASFFTAILAVQAISQVDPSVGVMVDVQNTLVNNAFMR
                     WGSDALKKKYLPQLATGKVGSYCLSESSSGSDAFALKTKAVKKGDKYILNGQKLWITN
                     AGEADIFIIIANLNPEAGYKGITAFVVERGFKGFSVSKKENKLGIRASSTCELVLEDC
                     EVPVENLLGEEGKGYKVAIETLNEGRIGIGAQMVGLAEGALQHAIDYTKTRQQFGKPI
                     SSFQGMQFQIAQMAIEVETSKLMVYNAARLKDAGKDFVKEAAMAKFHSSQVAERVASL
                     AVECFGGYGFVKEYPVEKLYRDAKIGKIYEGTSFMQLMTIGKILLG"
     gene            5934..6767
                     /locus_tag="PLF85_08590"
     CDS             5934..6767
                     /locus_tag="PLF85_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19763.1"
                     /translation="MYNRSKLFLFTVFVATSYLAPRTVEASCGMGVCPLPVAGGLNAQ
                     ALAGTGVLPSQLAVESRYVSFDIGGKGSYLQNAVSGVYEHRYFRAGGIVPLIYLSSPA
                     GETFGLGNTTLFGEVYLFTQPGTRLSVGSQLETPTGNHDKGLGADHFMAVPYINFWQM
                     FGDWRFAVQTGFQQTLGHHTHAATTTVLYVNPHTDTELMARAMASYTWQARYSAELGS
                     VWRQVMAHDAQGDKTFVDLGLAFRAALLDTLAVRAGVDVPLASRTRYLWQAYASVYAY
                     F"
     gene            6819..>7819
                     /locus_tag="PLF85_08595"
     CDS             6819..>7819
                     /locus_tag="PLF85_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19764.1"
                     /translation="MSPLARYSLYFACYVASGLAVLTTHYYQTSDYSQQSLDEQITLV
                     AASHFSLSQLVDNLPAVFRENPHAAALKISDLRGDFLGAMYDSRRMTAAEYKQFLALK
                     TFTPGAAAVEGFTSHIWESKRRKLRIVALSLPRMQFSEYLARMSHAAYLHYIIPLFLL
                     AGALGIYALHMFSGKALRAVKPLPRPPQSSPVRPVLTAAPVTRTRAHAWHLKPGVVAD
                     SAIRDALQALRQMTGATCVSLFARSGKKKNSAWTGVTELRGSIIVRGDSMDATGIDRT
                     ETTATVSSLNDSKTWCFFDGEYAEASLCFCLQFDRADQAPGDELRQRIVDFTRARARS
                     "
BASE COUNT         1831 a         2235 c         2154 g         1599 t
ORIGIN      
        1 gatatcgccc agcgattttt tggaaatgat caggattttt tgcatgttgg ccttgcgcgg
       61 cttgtgtgcg ccgcgctaaa aaagaattcc ggcgatggac gctgccaaaa tattagataa
      121 gacgcctacc acaacagcca gaaatcccaa agaggccagt tcggcccgcc gctgcggcac
      181 cagcgcccca atgccacccg cctggatcgc aatggatgta agactgccga aaccacagag
      241 catataggtc gaaatcagga atgcctttgg tgacagcacc tcgcgaattt tacccagttc
      301 gatgtagccc acgaattcgt tgagcacgat gcgcgtgccc agcaggttgc ctacctgcac
      361 agcctcgctc cacggtacgc cggcaacaaa agctacgggt gcaaacagca acccaagcac
      421 ctgctgtata gtcgccggaa tagcagcgaa aaaaccgtgc agggcaccgg atgtctgcgc
      481 gagcgccgct tcacgtgctg cggcccagat accattcacc agtgcaatga gcgcgatgaa
      541 ggcgatcagc atggctgcga cgttggccgc aagcagcagg ccttctgagg cgccgcgcgc
      601 ggcggcatcg atgaaattga cgggacggtt ttcgtccacg ggtggcggag cagcctgctg
      661 tgatcctgcc gcaccgccct gcgctgtctc gggcaaacca gtctcgggaa ataccatctt
      721 cgccagcatg atcgaacccg gcgcagtcat aataaccgac gccagcaggt gcttcgcgtc
      781 aacccctgcc accgccacgt agactatcag tattccggca ctgcagtgag ccatacctga
      841 caccatcacc gtgaaaagtt ctgaacgcgt aaggcctgca aggtagggct tgatggtgag
      901 cggcgcttcg gtctgcccca taaaaatatt cgccgcaacg tcgagtgttt cagcgcccga
      961 agcgcgcatg aaacgattga tgaactttgc gatcgcgcga atcagcggct gcatgacatt
     1021 gaggtaatag agcacggaaa aaatcgaagc aacaaagatg atcgtcggca acacctggaa
     1081 cgcgaatacg aacgcgatac ccgcggcttt atcaccgagc gctgtggtga aagatgcgcg
     1141 catcgcttct tctgaaccca aaaccccgaa aacaaaactt gcgccgacgc ctgaaaactt
     1201 gagcacctgc gcagcccctg tcttgagcga ctcgaaaatg gcgccaacaa cattaaacct
     1261 gagcaggtag aaaatcgcct cggcccatac gacgcgcctg acccatacca tcggcaggcg
     1321 cgcagcaagc gtgggagcgt acctagtgac gagccaaagc gcgagcacct ggcaggccac
     1381 tatggccagg aaaacagact gcgtcaaagg gaaaaaacta aagagctctg cgatactatc
     1441 gccgcgaatg attaacaggg caaagctgat ttgcagtaca agcccccaca agacggttcg
     1501 ccagtcgatt gccccgcgat tgcgcgatag aagtaaacac gaaataccga caaagataat
     1561 tcccaggacg ccagtgaggt ttaccatgat cacccttcga ccttcacgtt atggcgttcg
     1621 agttcttcat tggtcggacg caggacagcg ataagggcaa tgagcacacc gatcggaatc
     1681 agcaggttca tgtggtgcga aagaaaaaaa atcacagaaa taaaaagcgc ggccccctcg
     1741 agcattgccc actggactat cttcatggta aaatatttct gcagtggcac gtatttttcg
     1801 tcgcgcagca tgaaacgcgg taagagctgc gaaaaaccca ccgcgatcac cgccatcgcg
     1861 gcggccaccg actggaaaac aaccgactgc tcgccgggaa cagccgccgc cgcattaccc
     1921 gactgtatca ggaaatagac aactgcggca aaaattgccg gtgccagcat gagcgcgaga
     1981 tgaacgatgt agaagtgcgg tggaacgcgt gcaggtttca ttcgggcagc agcgccgatc
     2041 gtgcagcaga cgacaacaaa tttgcactcc cggcctgccg gggtctttgg atttacaggg
     2101 tggaaaagcc tgccagacaa gccaatggcc tacgagaaga tccgcctccc ggagacgggg
     2161 gagaagatcg cctggaatgg taaattacag gtgccggacc aaccgattat tccgtacatt
     2221 gagggcgacg gcaccggacc agatatctgg aaggccagtg tgcgcgtgct cgatgccgcg
     2281 gtagccaaag cctacggaca aaaacgtaaa atttcctggc tagaggtctt tgcgggtgag
     2341 aaatccaata acctgtacgg cgccaataca tggctgcccg aagagaccct cgaatgcatt
     2401 gccgaacaca aggttgcgat caagggaccg atgagtacac cggtcggtgg cggaatccgc
     2461 tcggtgaatg tcgcgctgcg gcagaagctc gacctctatg cctgcgtgcg accagtacag
     2521 tattttgacg gtgttccctc accggtgcgc aggcctgaag atgtcgacat ggtaatcttt
     2581 cgcgagaata cagaagacat ctacgccggc atagagtacg aacccggcag cgagggtgca
     2641 aagaagattg cccgcgtgct tgaagagacg gggctgataa aaaacgtgcg gtttcctgag
     2701 actgtatcgt acggtattaa accagtctcg cgcgagggtt cggagcgtat cgtgcgcgcg
     2761 gcgattgaat acgcgattgc aaacaaacgc aagtctgtta ccctggtgca caagggcaat
     2821 atcatgaagt acaccgaagg ttacttctgc aaatggggct acgagacggc gcgcaagtac
     2881 tttgctgctg aaaccgtcgc ctgggatgaa tgcggcggca accctcccgc aggaaaaatt
     2941 ctcatcaaag attctattgc cgacgctttc ctgcagcagg tattgttgcg cccgaaagaa
     3001 tacgatgtca tcgcaacgct gaacctgaac ggcgattacc tctctgacgc gctggcggcg
     3061 caggtgggcg gcattggcat tgcccccgga gcgaacatca actacctcac cggttcggcg
     3121 gttttcgaag caacacacgg tactgcaccc aaatatgccg ggctcgacaa agtaaatcct
     3181 tcatcggtga ttctttcggg tgaaatgatg ctgcgctacc tcggctggac agaagccgca
     3241 gactgtatca taagcgcgct gaagaagact attgcacaga agaccgtcac ctacgacttt
     3301 gcacgcatga tggaaggcgc aaaagagatc aagtgttctg agtttgcgga tgcgatcata
     3361 gctaacctct gattgccctt tgggcaatca gaggttagac aacccaaaat ctctctccct
     3421 cattaaaccg ggagacacca tctttcatgc tcttgctcgt cgcgaccggg taaaggttgc
     3481 gcgcttcgag tgtcagcgcc tctttcagcg gtaagtggct gccatttagt gccgacgaca
     3541 gatcagcgac gagtgcatcg cgcggctgtg cacagatctg tgctgccagg cgtaatgcgc
     3601 gttcgagacc cttacctttt cttacgagct cccacacaag gccaatttcg tacgcgcgct
     3661 gcgcacgaat gcgctgcccg gtaatgatga gtggcagcgc cgcaccccag ttcaaaagcc
     3721 tcggcaggta gacagtcccc ccgtcgacca atggcacccc ccagcgccgg cacgcgacag
     3781 aaaacagcgc ctgtggttcg gcgatgcgta tgtgcccgtg gcagaagagt tcaagcccgc
     3841 cggcatacgt gtaaccgtgg ctaacggtaa taaccggttt ctgctgcacg atgcggctgc
     3901 cccccatggg cccggggtcg ttctgcgcat aacgctcgag cgcggcggcg tcgggcgagg
     3961 tggagttcag aagaccgccg gccagcgtat cgagtgctgt cagatcggca cccgcgcaga
     4021 acgatttgtc acctgcgccg tgcagcacag caacggcaag cgaatcatcg tcccggaacc
     4081 tgagccatgc ttcgagcagg tattttgcca tttcgccgtt acaggcgttg tgtacctcgg
     4141 ggcggttcat cgtgatctgc aggatgttac ccccggtaga ttcaatgact ctcgtgagaa
     4201 cgggtttttc tgtcatattc taagggagat ttggacgtgc ctgcgtcaat attccttttc
     4261 atattgagtt atttcgccag ttttacccat gtcagcatta accattctct cagacgaaga
     4321 acagtcgtac tttgacgcga tcaaagattt tgccaaaaaa gaagtagctc cccatgtaac
     4381 ggccatggac gaagcgcaga aaatcaaccc cgacatcgtg aagaaactct tcgagatggg
     4441 cctgatgggc atcgaagtgc cgcaagaact ggaaggttcc ggggcatctt tctttacggc
     4501 gattctcgct gtgcaggcga tttctcaggt agacccatcg gtcggcgtga tggtcgatgt
     4561 gcaaaacacc ctcgtgaaca atgcattcat gcgctggggg tcagatgcgc tgaagaagaa
     4621 atacctcccg cagctggcga ccggcaaggt cgggtcttac tgcctgtcag agagctcttc
     4681 gggttccgac gcctttgcgc tgaaaaccaa ggcagtcaag aaaggcgata agtacatcct
     4741 caacgggcag aaactgtgga tcaccaacgc aggtgaagca gatatcttca ttattatcgc
     4801 caacctcaac cccgaagccg gttacaaggg tatcacggca tttgtggtcg agcgcggttt
     4861 taaaggtttt tcggtttcta aaaaagaaaa caagcttggt attcgtgctt catccacctg
     4921 cgagctcgtg ctcgaagact gcgaagtccc ggtcgaaaac ctgctcggtg aagagggcaa
     4981 agggtacaag gtagcaatcg agacgctcaa cgaaggccgt atcggtatcg gcgcgcagat
     5041 ggttggcctg gccgaaggtg ctttgcagca cgcgatcgat tataccaaga cacgccagca
     5101 gtttggcaaa ccgatctctt ctttccaggg tatgcagttc cagattgcgc agatggcaat
     5161 cgaggtcgag acttcaaagc tgatggtcta caatgcggcg cgcctcaagg acgctggcaa
     5221 agatttcgtc aaagaagcag ccatggccaa gttccactcg agccaggtag ccgaacgcgt
     5281 cgcttcgctc gccgttgagt gcttcggcgg ttatggtttc gtgaaagagt acccggttga
     5341 aaagctctac cgcgatgcca aaatcggcaa gatctacgag ggcaccagct tcatgcagct
     5401 gatgaccatc ggcaagattc tgcttgggta acgaatacct aagcaaacat tccacttgcg
     5461 gtactcacgg atcatagttt tcctggtgct ggcggttttt actgccagcg cctcggggtt
     5521 tgccgaatca ttcgcacacg cagaacacaa cgcgtggtcg aagtgccggc acacgcaaca
     5581 acgcattgcg cgcggtaaac cctgcccatg cggctgtaac aaaaaggcaa aacctgccct
     5641 acgcatcacg aatgatcata gcgactgcga tgccgacgac gtcgtcgctc acgccccggc
     5701 tttttctcaa ctggattctc ttttgagctc tgcggcgccg ctcgcgccaa ctctttcttc
     5761 tgtgctcagt ttcattacct ggccagaaat gcattcgctg ccgcggccac ctctcataaa
     5821 acctggataa atccccttcg ccggtctgcc catgcgcatg actggccctc atttttttgc
     5881 ctgtaacagc aggccgggca acgtcccgaa taatgaaagc ttaaggagat attatgtata
     5941 atcgctcgaa attatttttg ttcactgtat ttgttgctac aagctacctc gctccccgca
     6001 cggttgaggc gagctgcggt atgggagtct gccccctgcc ggtggccggt ggcctgaatg
     6061 cgcaggcgct cgctggcaca ggcgtactgc cctcacagct ggcagttgaa tcacgttacg
     6121 tcagtttcga catcggtggc aagggtagct acctgcagaa cgccgtttcc ggagtttatg
     6181 aacaccggta ctttcgcgcc ggcggtatcg tgcccttgat ctatctttcc agtccggccg
     6241 gagagacttt cggtttgggc aacacgacgc tgtttggcga ggtttacctg ttcacgcaac
     6301 cgggcacccg tctttctgtc ggcagccagc tcgagacacc aacgggaaac cacgacaagg
     6361 gtctcggggc cgatcacttc atggccgtgc cctatatcaa tttctggcag atgttcggtg
     6421 actggcggtt cgcggtgcag acgggatttc aacagaccct cggccaccac acgcatgcgg
     6481 caacaaccac cgtgctttac gtcaatccgc ataccgatac agagctgatg gcgcgcgcca
     6541 tggcaagcta tacctggcag gcacgctact cagcggagct cggttcagtc tggcgccagg
     6601 tgatggcaca cgacgcgcag ggcgataaga cattcgtcga cctgggcctc gcgtttcgcg
     6661 cggcgcttct ggacacgttg gccgtgcgcg cgggtgtcga cgtcccgctg gcatcgcgga
     6721 cgcgctacct ttggcaggca tacgcctcgg tctacgcgta tttctgaacc tcccgaagag
     6781 gcaagttcac gcgggccatt tccgataatg atatcggtat gagtccgctt gcccgctatt
     6841 ctctttactt cgcgtgttat gtcgcttcag gactggcagt cctgacgacg cactactacc
     6901 agacttcgga ctacagccag caatcgctgg acgaacagat aactctcgtt gcggcatcgc
     6961 acttctcact atcgcagctg gtcgacaacc tgcctgccgt cttcagggag aatccccatg
     7021 ccgctgcgct caagatcagc gacctgcggg gcgattttct cggcgcgatg tacgattcgc
     7081 ggcgcatgac cgcagcggaa tacaagcagt tcctggcact caagacgttc actcccgggg
     7141 cagctgcggt tgagggtttc acgtcgcata tttgggagag caaacgccgc aagctgcgca
     7201 tcgttgcgct gagcctgccg cgtatgcagt tctccgaata tcttgcgcgc atgtcgcatg
     7261 cagcttacct gcactacatc ataccgctgt tcctgctcgc tggagcgctc ggcatctacg
     7321 cgctgcacat gttcagcggc aaggctctgc gcgcagtaaa acctctgccc cgcccaccgc
     7381 aatccagccc cgttcggcca gttcttacag cggcacccgt aacaagaact cgcgcgcatg
     7441 catggcatct taagccaggt gtagtagcag acagcgccat ccgcgatgca ctgcaggcat
     7501 tgcgccagat gacgggggca acttgcgtca gcctctttgc gcgctcgggc aaaaagaaga
     7561 actccgcctg gaccggggtc actgaactgc gaggctccat tatcgtcagg ggcgattcca
     7621 tggatgccac gggaatcgac cgtaccgaga ccacggctac ggtttcttcg ctgaacgaca
     7681 gtaaaacctg gtgctttttt gacggcgaat acgccgaagc ttcattgtgc ttttgcctgc
     7741 agtttgaccg cgccgaccag gcccccggcg acgaactgcg gcagcgaatc gttgatttta
     7801 cgcgcgcacg tgcccgctc
//
LOCUS       DAOQJF010000137         7750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5139_length_7750_cov_6.411566, whole genome shotgun sequence.
ACCESSION   DAOQJF010000137 DAOQJF010000000
VERSION     DAOQJF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5139_length_7750_cov_6.411566"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1531)
                     /locus_tag="PLF85_08600"
     CDS             complement(47..1531)
                     /locus_tag="PLF85_08600"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNE19765.1"
                     /translation="MISLSILIPLVAGIVILLWREPAKDSRFAQRNPLIAIAVTLLSF
                     FSAIILQKRFAAGYGEAQLREVWWTITGSGINFSFALDGISMPLFLVTAFLFLIAALF
                     SYSVHRVSSVPLGKTFWGMLLVLEATVLGVFSAWNFVVFFIFWELFLVPITVLIWLYG
                     LEERKKAALNFFIYTFVSSAFMLAAFAAIVYYIPRIGADFDLRTTFAPEVQGIHPDKQ
                     RILFLFFVIAFLVKMPVFPFHAWLPLTHTQAPLGSLLLSGLFLKLGSYGLLRFVTPNF
                     TGVIAEWGQIFMYLGLASMFYGAFAAYRQKSFRNVIAYSSLAHMGLMLTAIFAQHEYA
                     LTGVIIQNVGHSLANALLFVVVVMHLARNKSDDLGTIAAPSSTFYWITFSIALFSAIG
                     VPGTIGFVGEFLMLLGISYRSWLLAALAVLTLVFSAAYMLRLFHKVRAQTPDTTWRPT
                     TIERLCMGLLVVAILWFGIAPGFVGENARSSAKTLSTVSPGVGR"
     gene            complement(1533..3506)
                     /gene="nuoL"
                     /locus_tag="PLF85_08605"
     CDS             complement(1533..3506)
                     /gene="nuoL"
                     /locus_tag="PLF85_08605"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNE19766.1"
                     /translation="MKPEHIPYLLIVVPLAPLLAAVINTAIFAWTERTKKVTAQAWTI
                     FITLSLTLLAGGAHAAVIHLHPSLNGEGTATIVSPATWMALVHPLRLSWAIRFDALTT
                     IMVPVVLFVGLMVQIFSVGYFHDRRQDIARYYAWLSFFLFSMLLLVVSANMFVFFVGW
                     EAVGLSSYKLISFYLEKDTAQKAGKKAFIMNRIGDAALLIAMFALFRVAGSADFREIA
                     LAFSRGSIAHSHANFIGLMVFIAASAKSAQIPLYTWLPDAMTGPTPVSALIHAATMVT
                     AGIFLMLRLEPVFALAPEASALVAYAGAGTALLAAFVAVGQTDIKKVLAFSTVSQLGL
                     MVLAVGTGAYAAAFFHLFTHAFFKAMLFLSAGAVITYLHHEQNIRKMGGLMRAHPLLG
                     ILFWVALAALAGIPGLSGFFSKDHILAAAFAAKQNGNLLGSVVVIVSLLTAFYSFRLG
                     ILVFHGKAQAHDSGHESHELPTVSFNLPLVLLAIPAVIAGFAGIPAFFTGNEPLFFEF
                     LNRNFPAAVYDVTQQMQWKLDHAAELRIITAGVAAALTGSLAAIFWYGILRRRPVAEG
                     AYRNGLVRASLRKLYIDEIYDFCFVKPYRAVADKLARWDATILPAVSEGIGLFVAEVS
                     GMIARLQTGVIAHYFTWIFAGIVVFLTLAMGIL"
     gene            complement(3503..3823)
                     /gene="nuoK"
                     /locus_tag="PLF85_08610"
     CDS             complement(3503..3823)
                     /gene="nuoK"
                     /locus_tag="PLF85_08610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNE19767.1"
                     /translation="MNVQDFLSVREIAVLAFVLFTIGVVGVVTRRNLIVILMSVELML
                     NSVNLLFVAFSRGFNDTTGQLTVLFVMAIAAAESAIGLALIIALFRNTRSVMTDAAQE
                     LKDS"
     gene            complement(3820..4392)
                     /locus_tag="PLF85_08615"
     CDS             complement(3820..4392)
                     /locus_tag="PLF85_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNE19768.1"
                     /translation="MKTARVEDISFRQTLFYCFAFILTGSALGVVFHRNPVKSALFLV
                     SFFVGLAAMYALIGAEILATLQVLVYVGAIMVLFLFVIMLVALREENFESPLSNIGRT
                     AIIATLSLAFLLQLILLLQIKPGRADLNPAGQVTTTLVAGKQVTDAAQVISINLFRDY
                     LIAFELISLLLLVAVVGAIMIAKKNRQALE"
     gene            complement(5255..6316)
                     /locus_tag="PLF85_08620"
     CDS             complement(5255..6316)
                     /locus_tag="PLF85_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19769.1"
                     /translation="MPQREYTRIDLKNPAQTIVEEERTVNLQHGTNQVEFAFSGVSVD
                     INSIQFRPIKTPGSVRVINVNYPPGESALFWEVYSDKAGPGVFRISYLIANLQRTTAY
                     EAVVAKDEKTLGLKAHTMVQNGSGELFSAAEFHSSIGKAYNGSLLQGESKKLLAFSAG
                     DVRFTKKYFFDQSVSDQVALVYEIRNEKSTGLPEVMLPNGKARVYQEDSQGSEAFLGE
                     DWTQETGAGQKLELKLGQAKEVKVTRAIVKRKEEVVKLPIKNFHQTIRYQVENFKDTP
                     VELTVTEHPGGEWAIDDIEVKEESGERDDRKERSYSTDVKTERIDLDNVRLYMKVPAN
                     GREKKKLNVYVKLVLKNRW"
     gene            complement(6455..>7750)
                     /locus_tag="PLF85_08625"
     CDS             complement(6455..>7750)
                     /locus_tag="PLF85_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19770.1"
                     /translation="TVPKSQAVQLTIYNSADITMVKESRELTFKPGLNTIQFSWAGTL
                     IDPTSLRLTFLTHKDKLTLRDTSFPPGRTDALQWNIDSELSGPAKVEINYFTSGITWN
                     ADYTAITNAKETAMNVDGFIRVVNNSGEDYPGAEVRLVVGTVNLVEDIAGLAQGSWRY
                     KDLDDDRRERVRNGFKKRVKKAEAAKMAMDEAEAMPAPSAPMAGASRPKEIVKEGLSE
                     YFLFTVEGKESIPNGWQKRLKAFSAKDVEVKAIYRASDRTTSGSVHKFYEFRNTKEKG
                     ADPKTSLGVSPLPDGTVHVFAEDAKNNLSYQGGVYMKYVATGDKVKLDAGETREVVLR
                     TFTRDFKRYGFVTDTRYDKTHYVKEWVDEYFYEHELENTLGRSVSVEIERTFPGNFEP
                     GKIPFKYEKVDSTTIKFFPEVDAREKKKIPYSVKVENKE"
BASE COUNT         1883 a         2105 c         2116 g         1646 t
ORIGIN      
        1 tatagaatca tgttgatatg cttacagccc ctgaaagtaa tagccattag cgtcctaccc
       61 cggggctcac cgttgagaga gtttttgccg agctgcgtgc gttttcacct acgaagccgg
      121 gcgcgatacc gaaccagagg atcgcgacca ccaacagccc catgcagagc cgctcaatag
      181 tcgtcggccg ccaggtggta tcgggcgtct gggcgcgcac cttgtggaac aggcgcagca
      241 tgtaagcagc gctgaaaacc agcgtcagaa ctgccagggc ggcgagcaac catgaacggt
      301 aagagatgcc gagcaacatc aggaattccc cgacaaaacc gatagtgcct ggaactccaa
      361 tcgccgaaaa aagcgcgatg gagaaggtaa tccaatagaa agtcgaagac ggcgcggcga
      421 tcgttcccag gtcgtcgctc ttgttgcgcg ccaggtgcat aacaacaacg acgaacagca
      481 gcgcgttcgc gagactgtgg ccgacatttt ggatgattac gcccgtgagt gcatattcgt
      541 gttgcgcgaa aattgccgtg agcattaagc ccatatgcgc aagcgacgaa taggcaatca
      601 cattgcggaa ggacttctgc cggtaggcag cgaaagcgcc atagaacatg gaggccagcc
      661 cgaggtacat gaagatttgt ccccactcag caatgactcc ggtaaaattc ggcgtcacga
      721 agcgcagaag gccatacgaa ccgagcttga gaaaaagacc cgaaagcaga agcgacccca
      781 gcggcgcctg cgtgtgcgtc agcggcaacc aggcgtgaaa aggaaacacg ggcatcttca
      841 cgaggaacgc gatcacgaag aataaaaaca ggatgcgctg cttgtcagga tggataccct
      901 gcacttcagg agcgaacgtt gtacgcaggt caaaatcggc gccgatacgc ggaatgtaat
      961 agacaatcgc ggcaaatgca gcgagcataa aggcagaaga aacaaacgta taaataaaga
     1021 agttcagggc agcttttttg cgttcttcga gaccatacag ccagatgaga acggtaatcg
     1081 gcacaaggaa gagctcccag aaaatgaaga acaccacgaa gttccaggca gaaaacacac
     1141 ccaggaccgt ggcttcgagc accagcagca tgccccagaa ggtttttcca agtggcacag
     1201 aggacacccg gtggacgcta tatgagaaaa gcgctgcaat caggaacagg aatgccgtga
     1261 cgagaaagag cggcatagaa ataccgtcga gcgcaaagct gaagttgatg ccactgccag
     1321 taatcgtcca ccagacttcg cgcagctgcg cttctccgta gccggcggca aaacgctttt
     1381 gcaggataat cgcagaaaag aatgagagca gcgtcaccgc gattgcaatc agtggattgc
     1441 gctgcgcaaa gcgagaatct ttagcgggtt cgcgccacag cagaatgaca atgccggcaa
     1501 ccaatggaat gagtatcgat agagaaatca tctcagagta tccccatggc gagagttaag
     1561 aagactacaa tgcccgcgaa gatccaggtg aagtagtgcg cgattacccc ggtctgtagt
     1621 cgggcaatca taccagaaac ttcggcaaca aaaagcccga tcccttctga aaccgctggc
     1681 aatatcgtag catcccatct ggccagcttg tccgctacgg cgcggtacgg tttcacgaaa
     1741 caaaagtcat aaatttcgtc tatgtagagt ttgcgcaggc tcgcgcgtac aagaccattg
     1801 cgataagcac cctcagcaac cgggcggcga cgcagaatac cataccagaa aatcgccgcg
     1861 agcgatcccg tgagtgcagc cgcaacaccc gccgtgatga tgcgcagctc ggcggcatgg
     1921 tcaagcttcc actgcatctg ctgcgtcacg tcgtaaacag ctgccggaaa gttgcggttg
     1981 aggaattcaa agaagagcgg ttcattgccg gtgaaaaacg ccgggatgcc cgcgaaaccg
     2041 gcgatgactg caggtatggc gagcaacacc agtggcaggt taaagctcac agtggggagc
     2101 tcatgcgatt catggcctga atcgtgcgcc tgcgctttac cgtggaagac caggatacct
     2161 aagcggaaac tgtagaaagc agtgagcaac gagacaatca ccacgacact gccgagcagg
     2221 ttaccattct gtttcgccgc gaaagcggca gcgagaatgt ggtccttcga gaagaaaccc
     2281 gaaagccccg gaatacccgc gagtgcggca agcgccaccc agaacagtat gccaagcaaa
     2341 ggatgcgcgc gcatcaggcc gcccatcttg cgaatattct gttcgtggtg caggtatgtg
     2401 atgacagcgc cagcggatag aaaaagcatc gctttaaaga aggcatgcgt gaagaggtga
     2461 aagaatgccg cggcataagc accggtgccc accgcgagaa ccatcaggcc cagctgggac
     2521 accgtggaga aagcgagcac cttcttgata tctgtttgcc ctacagcgac aaaggccgcc
     2581 agtagtgcgg tgcccgcgcc tgcatacgcc accagcgcag aggcttcggg ggccagcgcg
     2641 aaaaccggct cgaggcgcag catcaggaag ataccggcag ttaccatcgt cgccgcatgt
     2701 ataagcgccg aaaccggtgt cgggcctgtc atagcatcag gcaaccatgt ataaagcgga
     2761 atctgcgccg acttggccga agcagcgata aaaaccatca acccgataaa attagcatgg
     2821 gaatgcgcta tactgccgcg cgaaaaagcc agtgcaattt cacggaaatc ggcgctaccc
     2881 gccacgcgga acagggcaaa catcgcgatc agcagcgccg catcgccgat acggttcatg
     2941 ataaaagctt ttttacccgc tttttgcgcg gtgtcttttt cgaggtagaa ggaaatcaac
     3001 ttgtaagaag acaatcccac tgcttcccag ccgacgaaaa aaacaaacat gttggccgac
     3061 acgaccagta gcagcatgct gaagagaaag aagctcagcc acgcatagta gcgcgcgata
     3121 tcctgcctgc ggtcatgaaa ataaccgacg ctgaagatct gcaccatcag gccgacgaac
     3181 agcaccaccg gcaccatgat ggtagtcaga gcatcaaagc gtatcgccca gctgaggcgc
     3241 agggggtgaa ccagcgccat ccaggtcgca gggctaacga tggtcgccgt gccttcccca
     3301 ttgagcgatg gatgcaggtg tatgaccgcc gcatgcgctc cgccagcgag cagcgtcagg
     3361 gaaagagtaa tgaatatggt ccaagcctgc gctgtaacct ttttagtccg ctcggtccac
     3421 gcaaagatcg cggtgttgat cacagccgca agcaaaggcg ccagcggaac gacaatgagc
     3481 aggtacggta tatgctcggg tttcatgaat ccttgagctc ctgcgcggcg tcggtcatga
     3541 cgctgcgcgt attccggaaa agcgcaatga tcagtgccaa gccgatggca gactctgcag
     3601 ccgcgatggc catgacgaag agtactgtca gctgtcccgt cgtgtcgtta aaaccgcgcg
     3661 agaaggcgac gaagagcagg tttactgaat tgagcatcag ctcaacggac atcaggatca
     3721 cgatcaggtt gcgccgtgtc acgacgccca caacgccgat ggtaaagagc acgaaagcca
     3781 gtacggctat ttcacgtacc gagagaaagt cctgtacgtt cattctagcg cctgccggtt
     3841 cttttttgca atcatgatag cccccactac tgcgacgagc agcagtagag aaatgagttc
     3901 aaaagcaatg agataatcac gaaaaaggtt aatcgagatg acctgggcgg catcggtaac
     3961 ctgcttacct gccacgaggg ttgtggtgac ttgtcccgcg gggtttaggt ctgcacgacc
     4021 gggtttgatc tggagcagca gaatcagttg cagcagaaac gccaatgata gagtagcaat
     4081 gatggcggtg cggccgatat tgctgagcgg gctctcgaag ttctcttcgc gcagcgcaac
     4141 cagcatgatg acaaaaagaa acagcaccat gatagctccc acgtagacca aaacctgcag
     4201 cgtagcgaga atttcggcgc caatgagggc gtacatggca gcaagaccaa cgaaaaagct
     4261 gaccaggaag agcgccgact tgaccggatt acggtggaaa accaccccta acgcagaacc
     4321 ggtaaggata aaggcaaaac aataaaagag ggtttgccta aagctaatgt cctccacacg
     4381 tgcggtcttc ataatcggca ttcggcgcaa agcagaaatg tgggatcggg gagttgggtg
     4441 cgccaatggt caggtagccg gaatattggc gaacagattc aggagagccc cctcgatgtc
     4501 ggatgcgact tgtgcgctag gcgctagatg tttcattcat cgattacgtc ggatcacggg
     4561 atgcaagggg attttctcct caaatagttg tcttttggac agaatgcgat attcctgcac
     4621 atatttcagt ccgccagaga aattgtgctc tgtgcgacat tggtaggtaa caaatacctc
     4681 gagccttggc gcatgtctgc agccatagcg cgcctgccaa tatctatagt tcctttgtgc
     4741 ccgccggcaa tccggcatag gactccgcaa gacctcttct agcaaccgcg gcatatatgt
     4801 acgtatggca aaatcgagca tccgtgaaat cgattcgcct gaatggatcg cacgctgcca
     4861 aagtacggag tacagcatct tgttcacata ccaggccatt cgaatatagg gaacacctac
     4921 cgagtcgttg tagcgccttg ccgtcgcaga tttcagcttt tccccacgcc aatgccgtaa
     4981 gaaaaccttc gctagttgtt tcaatagctc agactctgac cactcgacac cgttgcgacc
     5041 caagactttc cgagcagccg tgagacgcct tttcgatctc aggcggagag agaccgaggt
     5101 gaggtggtac tcgtcggggg atattctttg catcctacat attaaatgcc aagaaatcgc
     5161 cgttttttct cacctattcg gcattttttt caattttctg attttcttag taactaccaa
     5221 tatccaaaat tccgcgattt tacctctccg cgtttcacca ccggttcttg agcaccagct
     5281 tcacataaac gttgagcttc ttcttctccc ggccattcgc cggtactttc atatacagcc
     5341 gcacattatc gaggtcaatc cgctcggttt tgacatcagt tgaatacgag cgctctttgc
     5401 ggtcgtcgcg ctcaccgctt tcttctttta cttcgatatc atctatcgcc cactcaccgc
     5461 ccgggtgctc ggttaccgtc agctcaaccg gggtgtcttt gaaattttcg acctggtaac
     5521 gaatcgtctg gtggaagttc ttgatcggca gcttgacgac ctcttctttg cgtttcacga
     5581 tggcccgggt caccttgacc tctttggcct ggccgagctt cagctcaagc ttctggcccg
     5641 ccccggtttc ctgcgtccag tcttcgccca aaaaggcttc tgacccctgc gagtcttcct
     5701 ggtagacgcg ggccttaccg ttgggcagca tcacctcggg cagcccggtg gatttttcat
     5761 tgcgtatctc gtaaaccagc gcgacctggt ctgagaccga ctggtcaaag aagtactttt
     5821 tcgtgaagcg cacatcgccc gcagaaaagg cgaggagctt cttcgattca ccctgcagca
     5881 ggctgccgtt gtaggctttg ccgatgctgc tatgaaattc tgctgcggag aaaagttcgc
     5941 ccgacccgtt ctgcaccatg gtgtgcgcct tgagaccgag ggtcttctcg tctttggcca
     6001 ccactgcctc gtatgcggtg gtgcgctgca ggttcgcaat caggtacgag atacgaaaca
     6061 ctcccggccc ggccttgtct gagtagacct cccagaacag cgcactctct cccggcgggt
     6121 agttgacatt gattacgcgc acactgcccg gcgttttgat cgggcgaaac tgtatactgt
     6181 tgatgtcgac cgacacaccg ctgaaggcga actcaacctg gttcgtgcca tgctgtaagt
     6241 ttaccgtgcg ctcttcttcg actatggtct gcgccggatt cttgagatcg atgcgcgtgt
     6301 actcgcgctg cggcatggta acaagctttg tgcgggcaga tatactgccg actagcagca
     6361 aactggtgag tgcggccaga ccccaccccc ggcccctccc cttgataagg ggaggggtgc
     6421 cgaaggcggg gaggggttta gtctgtttca taaattactc cttgttctcc actttgaccg
     6481 aataggggat cttcttcttt tcgcgggcat ctacctcggg aaagaatttg atcgtggtcg
     6541 aatcgacttt ttcatacttg aagggaattt tccccggctc gaaattgccg gggaatgtgc
     6601 gctcaatttc gacgctgacc gaacgcccaa gtgtattctc gagctcgtgc tcgtagaagt
     6661 actcgtcgac ccactctttg acgtagtgcg tcttgtcgta gcgtgtgtcg gtcacgaaac
     6721 cgtagcgttt gaagtcacgc gtgaaggtgc gcagcactac ttcacgtgtt tcacctgcat
     6781 caagcttcac cttgtcgccg gtagcgacat acttcatgta gaccccaccc tggtacgaaa
     6841 ggttgttttt cgcgtcttcg gcgaagacat gcaccgttcc gtcgggcaaa ggcgatacgc
     6901 cgaggctggt ttttgggtcg gcgccctttt ctttcgtgtt gcggaactcg tagaacttgt
     6961 gcacactgcc gctggtggtg cggtctgatg cgcggtagat ggccttgact tcgacgtctt
     7021 tggcgctgaa cgccttgagc cgcttctgcc agccattagg gatactctct ttgccttcaa
     7081 cagtaaagag gaaatattct gacaggcctt ctttgacgat ctcttttggc cggcttgcgc
     7141 ccgccatcgg agccgaaggt gcaggcattg cctcggcttc atccatcgcc atcttcgcgg
     7201 cttctgcttt tttcacgcgt ttcttaaaac cattgcgcac gcgctccctg cggtcgtcgt
     7261 cgaggtcttt gtagcgccag ctgccctgcg cgagacccgc aatgtcttca acgaggttca
     7321 cggtaccgac aacgagcctc acttcggcgc cggggtagtc ttcacccgag ttgttcacca
     7381 cgcgtatgaa gccatcgaca ttcatggccg tctctttcgc gttggtgatc gccgtgtagt
     7441 cggcgttcca ggtgataccc gaggtgaagt agttgatctc tacctttgcg ggccctgaaa
     7501 gttcgctgtc gatattccat tgcagcgcat cggtgcgccc cggtggaaac gaggtatcgc
     7561 gcagcgtgag cttgtctttg tgcgtgagga aagtgagccg cagcgaagtc gggtcgatca
     7621 acgtaccggc ccacgagaat tgtatagtgt tcaagcctgg tttgaaggtg agttcccgcg
     7681 attctttgac catcgtaata tcggcgctgt tgtagatggt cagctgcacc gcctggcttt
     7741 tcggtacggt
//
LOCUS       DAOQJF010000138         7749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5142_length_7749_cov_5.184689, whole genome shotgun sequence.
ACCESSION   DAOQJF010000138 DAOQJF010000000
VERSION     DAOQJF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5142_length_7749_cov_5.184689"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLF85_08630"
     CDS             <1..318
                     /locus_tag="PLF85_08630"
                     /inference="COORDINATES: protein motif:HMM:NF036800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19771.1"
                     /translation="LLSIKNAELQTKLFREITDQGLNARAIEARARVLAAGKEEKKPL
                     KGKKADANIKAVAQKVEEHTGMRTRINYNPQKKSGSITIDFFSPDDLEKLLKMMGMKR
                     ISL"
     gene            323..1420
                     /locus_tag="PLF85_08635"
     CDS             323..1420
                     /locus_tag="PLF85_08635"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNE19772.1"
                     /translation="MAQLPNFLNSITQQAAHYTPGEQVNEPGWIKLNTNESPLPPSPL
                     VFKALAELVGQGDILRKYSHPLGEPLLSALAAYWQTDSKNILVTNGSDEALALLCRAT
                     LSAERPAAFPAITYSLYQTLVNNCGAHFVELPMVSKVGQPYSVDLQALAVCEAPVIFL
                     ANPNAITGEYIPAAQLAQVIAQSQKLWIIDEAYNDFAGPDATMMKHIAQLPNLVVTRT
                     FSKTHALAGLRVGYLATTNSSLMNGLLAHKDSYNEDAIATRLAAAALGDRDWHGKILR
                     SVASGKEFLRAELAVLGFTVYDSEANFMLAKAPAQLAADKIVQRLRDYKILIRYYAGS
                     AFADCVRISVGSEGENAKLIGSLKEILKATP"
     gene            1417..2445
                     /locus_tag="PLF85_08640"
     CDS             1417..2445
                     /locus_tag="PLF85_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNE19773.1"
                     /translation="MRYRVLSWLRLSKKAPATVAEQPKDWYEQHIVTLTRHERFRERP
                     GGIPFFRNKPVWIHLYPRNERDLIELLHRYDIHPLTVEDIFNSSNRIKREKFPHYTYY
                     CFRGVHLAGSYIATLDFNFIITKKTLITISESERSTIDRLLADNTLARELLRKGPEFI
                     LHRILDVETDHTLRIVHEIDLAFERCESALLTHSADLDIAMVFELKSSLATIKKLIIT
                     HKEIFDEMQQYDIAHFSPESAAFFRDVRDHAIKIIDTIDGIVQAIASAIEAYHTISTK
                     RTNDIIRILTIMTAIMLPLTLVTGYFGMNFEHLPLTKDHYGYLKTIIGMAALAVAMLF
                     WFVRKKWL"
     gene            complement(2450..2875)
                     /locus_tag="PLF85_08645"
     CDS             complement(2450..2875)
                     /locus_tag="PLF85_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19774.1"
                     /translation="MPRATAWKYGLLLATVGAFALPNKAPVATSWISKNFVGGPRCVA
                     RGPASAFSPPGFESEQEKLGRQGIHVAQAYYRDLPTCQACEVCPNYRREILFEVRSAD
                     TDLAAKAGYNKVAAPESTELLEFQRSKIYRPPPDVPEND"
     gene            complement(2881..3666)
                     /locus_tag="PLF85_08650"
     CDS             complement(2881..3666)
                     /locus_tag="PLF85_08650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE19775.1"
                     /translation="MTDFRNRYGAWALITGASSGLGAEFARQLAAEKMDLVLVARRRD
                     RLNELATSLKAEHGIQVKVVPLDLSKPNFLAALQKQTAKLNIGLLVNNAGFGIAGDFT
                     TNSLERELMMLDVNCRAALQLAHAYGSQMAAHRRGGIIMVSSVVAFQGVPFMSHYAAT
                     KAYDLVLGEGLWHEMKKYGVDVVTLCPGATTTEFANVADTKPAPGSMPAPPVVRAALE
                     ALGKKHYVTAGKKNKLMVFSARLVPRRVATLIAARVMKSLGRY"
     gene            3924..5360
                     /gene="glnA"
                     /locus_tag="PLF85_08655"
     CDS             3924..5360
                     /gene="glnA"
                     /locus_tag="PLF85_08655"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNE19776.1"
                     /translation="MAKLKFGSWKEVIDFAKKSGVLFYDFRFSDIGGAWHHVSYHVDS
                     ITEDSFKGLPFDGSSITKWQPIHRSDMQLIPDTEGAFMDPFTADPTLVIFCDVYDIYK
                     NQMYEKCPRSIAKKALEYLKSTGLGDTAYFGPENEFFIFDSIRVRDDINCQYYEIDSN
                     EGAWNTATAQYNDGHNLGHRPGTKGGYFPVSPRDSQVDIRAEIVKTLHEVGLETFVVH
                     HEVAQAQGEIGVKFGTLIEAADNVQKLKYVVKNVAKRHGKTATFMPKPLFGDNGNGMH
                     VHQSIWKGGENTFAGDKYQGLSDTALHYVGGVLKYARAIAAFSNASTNSYKRLIPGFE
                     APSILTYSAQNRSASCRIPFVSGVKAKRVEFRFPDSTANPYLCFAAMLMAGLKGVQDK
                     IDPGKPMEEDLFELSLDEIREKGIQQMPHTLREAVETMLTNRGIFEQGGVFTKEFIDT
                     YKELKFESEIWPWEARPHPFEFLSTYSC"
     gene            5501..5944
                     /gene="dut"
                     /locus_tag="PLF85_08660"
     CDS             5501..5944
                     /gene="dut"
                     /locus_tag="PLF85_08660"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNE19777.1"
                     /translation="MSSITVKFTKLNPGAMLPGRQSVHAAGYDVHACLEHNITIKRGS
                     ITPVPTGIAVEIPPGYHISIRARSGMAVKHGLTPVNTPGTIDADYRGEIFVPLINLGN
                     EDYTIKNGDRIAQMLLEKTYEIYWQEGSLSETARGEGKFGSTGKR"
     gene            5922..7631
                     /locus_tag="PLF85_08665"
     CDS             5922..7631
                     /locus_tag="PLF85_08665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNE19778.1"
                     /translation="MVPQVSADPAGIVDYPRRLVKDQLYFAFGGGVGRFGTNFTVFHL
                     NGVTIWIDVGSGFANHHTPGMDKNLPNRQLLLGFPPTAIFLTHGHEDHIGALPHLTEV
                     IPVGTPIFASPFTTALVTARLKDRGIDPARWDFRLIEQDSDIEIGPFRVSTFFMPHSI
                     PQCFSVGLEASLPQGKKRIYFSSDFKMRGSEVRHKVQNIKKFAPVDFLFVDSTGSLHE
                     GETTDEREVDESLERLISRIPGRIFITTFASQVERIKNIVRIASGLSRPVGFLGRSLK
                     SQWEAAFISREVAAPLHLQKPPSYTSQNAVWLVAGCQADKNSSFSRLTHGTLAKVRLK
                     AGDTLVYSASMIPGNEGKIYEALNLAADVGVRVVGVSGDTRVHASGHGKRGDIERLIS
                     YLKPRHILPVHGDPLHFHAFIDFIDQKKLPVTVTEGHRIYALGDAPVLVESVPDETCL
                     VEAGEIHFDTTLYRERNHLAEQGVCFVALEPDRFAVSAVSYVGVMSAHFQETMYPRLL
                     VAAQEAAIAAGQSAPNNREKKLRDFLGKLHEELIGKNPYIKIVIPSPGQQAAAAERAG
                     GEV"
     gene            7628..>7749
                     /locus_tag="PLF85_08670"
     CDS             7628..>7749
                     /locus_tag="PLF85_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNE19779.1"
                     /translation="MNALRIIPCLDVKDGRVVKGVNFVNLRDAGDAVECAQAYDR"
BASE COUNT         1747 a         2174 c         2111 g         1717 t
ORIGIN      
        1 ctcttatcca tcaagaacgc cgaactgcag acaaaacttt ttcgcgaaat cactgaccag
       61 ggtttaaatg cgcgtgcgat tgaagctcgc gcgcgcgtgc tggctgccgg aaaagaagaa
      121 aagaagccgc tcaagggtaa aaaagctgat gccaatatca aggcagtagc tcaaaaagtc
      181 gaagagcata ccggtatgcg cacacgcatc aattataacc cgcagaagaa aagtggcagc
      241 atcacgattg atttcttcag cccggatgac ctcgaaaaac tgctcaagat gatgggcatg
      301 aaacgtatat ccctctagga ctatggcaca actaccgaat ttcctcaaca gtatcacgca
      361 gcaggcggcg cactatacgc cgggtgagca ggtcaacgaa cccggctgga tcaaactcaa
      421 tacgaatgag agcccgctgc cgccttcgcc gctggtattc aaagccctcg cggagctcgt
      481 ggggcagggc gatatactgc gcaagtacag ccacccgctg ggtgagcctt tgctttcagc
      541 cctcgcagct tactggcaga ccgatagtaa aaatatactg gtgaccaacg gctccgacga
      601 agcgctcgcg ctgctctgcc gcgccacgct gtcggccgag cggcctgcgg cttttcccgc
      661 tattacctac agcctctacc agacgctcgt caataactgc ggcgcgcact ttgttgagct
      721 gccgatggtg agcaaagtcg gccagccgta ttcggtcgat ctgcaggcgc ttgctgtctg
      781 tgaggccccg gtgatttttc tcgccaaccc caacgcaatt accggggaat atattccggc
      841 ggcacagcta gcgcaggtga ttgcgcagtc gcagaaactc tggattatcg acgaggccta
      901 caacgacttt gccgggcctg acgcgacgat gatgaaacac atcgcgcagc tgcccaacct
      961 tgtggtgacg cgcactttct ccaaaacaca cgcgctggcg ggcctgcggg tgggttacct
     1021 cgcaaccacg aacagttcgc tgatgaacgg tctgctcgcc cacaaagatt cgtacaacga
     1081 agatgctata gcaacgcgcc tcgctgccgc agctctcggt gaccgcgact ggcatggcaa
     1141 gattctgcgc tccgtggctt cgggtaaaga attcctgcgc gctgaacttg ctgtactggg
     1201 ttttactgtc tatgattcag aggccaactt catgctcgcc aaggcgccgg cacagctggc
     1261 ggccgacaag atcgtgcagc gcctgcgcga ctacaagata ctgatccgct attatgccgg
     1321 gtcggcattt gccgactgtg tgcgtatcag cgtggggtct gaaggcgaaa acgccaaact
     1381 catcggctcg ctcaaagaga ttcttaaggc aaccccatga gataccgcgt tctgtcgtgg
     1441 ctgcgcctta gcaagaaggc gcccgctacc gtggcagaac agcccaaaga ctggtacgaa
     1501 cagcatatcg tgacgctgac gcggcacgag cgttttcgcg agcgcccggg tggcattccg
     1561 ttctttcgca acaagccggt ctggattcac ctttatccgc gcaacgagcg agatctgatt
     1621 gaattactgc accgctacga tattcacccg ctcacggtag aggatatctt caacagttcg
     1681 aaccgtatca agcgcgaaaa atttccgcac tacacctact attgcttccg gggggtacac
     1741 ctggccggca gttatattgc tacgctggac ttcaatttta tcatcacgaa gaagaccctg
     1801 atcacgatct ctgagagcga acggtctacg atcgaccggc tgctggctga taatacgctg
     1861 gcccgcgaac tcttgcgcaa aggccccgag tttatcctgc accgcatact cgacgtagag
     1921 actgaccata cgttgcgcat tgtccacgaa atcgacctgg cctttgagcg ctgcgagagt
     1981 gcgctgctga cgcatagcgc cgatctggat attgcgatgg tatttgaact caaatcatcg
     2041 ctcgcgacaa tcaagaagct gatcatcacg cacaaagaga tattcgacga aatgcagcag
     2101 tacgatattg cgcatttctc ccccgagtcc gcggcattct tccgcgatgt acgcgaccat
     2161 gcgatcaaga tcatcgacac gatagacggt attgtgcagg caatcgcttc ggcgatcgag
     2221 gcctaccaca cgatctcgac taaacgcacg aacgacatca tccgtatcct gacgatcatg
     2281 accgcgatca tgttaccgct gacgctggta accggttact tcggcatgaa ttttgaacac
     2341 ctgccgctta cgaaagacca ttatggctac ctgaaaacaa tcattggcat ggctgcgctg
     2401 gcagtcgcca tgctattctg gtttgttcgc aagaaatggc tttaagaaat cagtcgtttt
     2461 cggggacgtc cggcggcggc cgatagatct tgctgcgctg gaactcgagc agttctgtgc
     2521 tttcgggcgc agctaccttg ttatacccgg cttttgcagc gagatctgta tcggctgaac
     2581 gtacctcaaa gagaatttcc cggcggtagt tggggcagac ttcgcacgcc tggcaggtcg
     2641 gtaggtcgcg gtagtaggcc tgcgcaacgt gaatgccctg tctgccgagc ttctcctgtt
     2701 cgctttcaaa ccctggcgga gaaaaggcag atgccgggcc gcgtgctacg caccgggggc
     2761 cgccaacaaa gtttttgctg atccagctgg tggctacagg cgctttgttc ggcaaggcga
     2821 aggcaccgac cgtcgcgagc agcaggccat atttccatgc ggtggcgcga ggcattgcgg
     2881 tcagtacctg ccgagacttt tcataacgcg cgcagcaatg agagttgcta cgcggcgcgg
     2941 taccaggcgt gctgagaaga ccatcagctt attcttcttc cctgcggtaa cgtaatgctt
     3001 tttgccgagt gcctcgaggg ctgctctgac aacgggtggg gcaggcatcg aacccggcgc
     3061 cggcttcgtg tcagcgacat tggcaaattc ggttgtggtg gcgccggggc agagtgttac
     3121 aacatcgaca ccgtattttt tcatttcatg ccagagtccc tcgccaagca ccagatcata
     3181 ggctttggtc gcggcgtagt ggctcatgaa cggcacgccc tggaaagcca cgaccgaact
     3241 caccatgata atgccaccgc ggcggtgggc tgccatctgc gaaccataag cgtgtgcgag
     3301 ctgcagcgct gcgcggcagt tgacgtcaag catcatgagc tcccgctcaa ggctattcgt
     3361 cgtgaagtcg cccgcgatgc cgaagccggc attgttgacg agcaggccaa tattcagttt
     3421 cgccgtctgc ttctgcagcg cggcgagaaa gtttggtttc gaaaggtcga gtggtacgac
     3481 ttttacctgt ataccgtgtt cggccttcag tgaagtggcc agttcattca accggtcgcg
     3541 ccgccgggca accagtacaa gatccatttt ttctgcagcg agctgcctgg cgaattcggc
     3601 tccgagcccg ctcgatgccc cggtgatcag tgcccatgct ccgtaacggt ttcgaaaatc
     3661 tgtcatatgc gtgcctgtgt ttcccagttg tggtgctcaa gccagtgcgt caagtttgtg
     3721 tctttccgtc attaatcgtg gccggcagat ttgccgtgta tgtttcgtaa ctggtagaaa
     3781 tcccattcgg ttgacaatgt gtctagaagg cacacctttc cttaggtctg cggtttggca
     3841 tcaagtttgc taaattactg gcaagaagga ttatccccgt tacgcggtgg cgcatcctac
     3901 aaacacaaca tacaggagat tttatggcta aattaaagtt cggctcatgg aaagaagtga
     3961 tcgattttgc aaaaaaaagc ggcgttctgt tctatgactt tcgcttcagc gatatcggcg
     4021 gcgcatggca ccacgtgtct taccacgtag actctatcac agaagactct ttcaaaggcc
     4081 tgccctttga cggcagctcc atcacgaagt ggcagccaat ccaccgctca gacatgcagc
     4141 tgattcccga tactgaaggc gcattcatgg atccgtttac cgctgatccg acgctggtga
     4201 ttttctgcga tgtttacgac atctacaaga accagatgta cgaaaagtgc ccgcgttcga
     4261 ttgctaaaaa ggccctcgag tacctcaaat ccacaggcct cggcgacact gcatacttcg
     4321 gccccgagaa cgaattcttc atcttcgact cgattcgtgt gcgcgacgac atcaactgcc
     4381 agtactacga aatcgactcg aacgaaggcg cgtggaacac agcgactgcg caatacaacg
     4441 acggccacaa cctcggccac cgtcctggta ccaagggtgg ttatttcccg gtttctccgc
     4501 gcgactcaca ggtagatatc cgcgctgaga ttgtaaagac actgcacgaa gttggtctcg
     4561 aaactttcgt ggtgcaccac gaagtggcgc aggcacaggg tgaaattggt gtgaagttcg
     4621 gcacgctgat cgaagcggct gacaacgtcc agaagctgaa gtatgtggtt aaaaacgtgg
     4681 cgaagcgcca tggcaaaact gctactttca tgccgaagcc actcttcggc gataacggca
     4741 acggtatgca cgttcaccag tctatctgga aaggcggcga aaacaccttt gccggcgaca
     4801 agtaccaggg cctcagcgat acagcgctgc actacgtcgg cggagttctc aagtacgcac
     4861 gggcaatcgc tgcattctcg aatgcttcga ccaactcgta caagcgcctg attccgggct
     4921 tcgaagctcc ttcgatcctg acgtactcag cgcagaaccg ttcggcttca tgccgcattc
     4981 cgttcgtctc gggcgtgaaa gcgaagcgcg ttgaattccg tttccctgat tcaaccgcaa
     5041 acccgtacct ctgctttgca gcgatgctga tggcaggcct caagggagtg caggacaaaa
     5101 tcgatccggg caagccgatg gaagaagacc tgttcgaact gtctctcgac gagatccgtg
     5161 agaagggtat ccagcagatg ccacacacgc tgcgtgaagc ggtcgagacc atgctgacca
     5221 accgcggtat ctttgagcag ggtggagtgt tcacgaaaga gttcatcgac acctataaag
     5281 agctgaagtt cgaatcagag atctggccgt gggaagcacg tccgcatccg ttcgagttcc
     5341 tctctactta ctcttgctaa tcggttcgtt tcggctccgc tcaacgaacc tctagaggtc
     5401 gctgagcgca gtcgaagtga ccttacgaca aaagggcctc agggcccttt tcttttaggg
     5461 ttttacgccc tgactcgacc caagctttct gcgcggggcg atgagttcaa ttaccgtcaa
     5521 atttactaag ttaaaccctg gcgcgatgct tcccggaagg caatctgtac acgcagcagg
     5581 ctacgatgtc catgcctgcc ttgagcacaa tatcaccata aaacggggca gtatcacccc
     5641 ggtgccgact ggcattgcag tcgaaattcc cccgggctac catatttcga ttcgcgcacg
     5701 ctcaggtatg gctgtcaaac atggcctgac gccggtgaat acaccgggta cgatcgatgc
     5761 cgattaccgc ggtgaaatat ttgtgccatt gattaacctc ggtaacgaag actatactat
     5821 taaaaacggc gaccgcattg cccagatgct gctcgagaag acgtacgaaa tctactggca
     5881 agagggaagc ttgagcgaaa ccgcgcgggg tgaagggaaa tttggttcca caggtaagcg
     5941 ctgatcccgc tggcattgtc gattacccgc gtcggctggt caaagaccag ttgtattttg
     6001 ccttcggcgg cggtgtgggt agatttggca ccaacttcac tgtttttcat ctcaatgggg
     6061 tcaccatctg gatcgacgtc ggttcgggtt ttgccaacca ccatacaccc ggcatggaca
     6121 agaacctgcc caaccggcag ctgttgctgg gttttcctcc caccgcaatt ttcctgacgc
     6181 atggccatga agaccatatt ggcgcgctgc cgcacctgac cgaagttatt ccggttggta
     6241 caccgatctt cgcctcgccg tttaccacgg cgctggtgac cgcgcggctc aaagaccgcg
     6301 gcattgatcc cgcgcgctgg gattttcggc tgatcgagca ggattcagac atcgagattg
     6361 ggccctttcg cgtgtcgacc ttcttcatgc cgcactcaat accgcagtgc ttctcggttg
     6421 gactcgaagc cagtctgccg cagggaaaaa aacgcatcta cttcagcagc gacttcaaga
     6481 tgcgcggttc agaggtgcgg cacaaggtgc agaatattaa aaagttcgca ccggtagatt
     6541 ttctctttgt cgattcgacc ggttcgctgc acgaaggtga gacgaccgac gagcgcgagg
     6601 tagacgagtc gctcgaacgc ctgatctcgc gcatacccgg tcgcattttt atcaccacat
     6661 tcgcctcgca ggttgagcgt atcaagaata tcgtgcgcat tgcctcgggc ctctcgaggc
     6721 cggtaggctt tctcgggcgt tctctcaagt cgcagtggga agctgccttc atctcgcgcg
     6781 aagttgctgc tcctctgcac ctgcagaaac cgccttcgta tacttcgcag aatgccgtgt
     6841 ggctcgtcgc gggttgccag gcagacaaga actcctcgtt ttcacgcctc acgcacggca
     6901 cgctggcaaa ggtacgcctg aaggcgggtg acacgctggt ctattcggcc agtatgattc
     6961 ccggtaacga aggcaaaatc tacgaagcgc tgaatcttgc cgccgacgtg ggtgtgcgcg
     7021 tggtgggagt ttccggcgat acgcgtgtgc acgctagcgg gcacggcaaa cgcggcgata
     7081 tcgaacggct gatatcgtat ctcaagccgc gccatattct gcccgtacac ggcgacccgc
     7141 tgcattttca tgcctttatc gattttatcg accagaagaa actgccggtc acggtcaccg
     7201 aaggacaccg tatttatgca ctgggcgatg cgccggtgct ggtcgagtca gtgcccgacg
     7261 aaacctgtct cgtcgaagcg ggtgaaatcc actttgacac aacgctctac cgtgagcgca
     7321 atcacctggc cgaacagggt gtctgctttg tcgcgctcga gcctgaccgt tttgcagttt
     7381 cagcagtctc gtacgtcggc gtgatgagcg cacacttcca ggaaaccatg tacccacgtt
     7441 tgttggttgc cgcgcaggag gcagcgattg cggcagggca gagcgccccg aataatcgcg
     7501 aaaagaagct gcgtgatttc cttggaaagc tccatgaaga gctgattggt aaaaatcctt
     7561 atataaaaat tgtgatccct tccccgggcc agcaggctgc agcagcggaa agggcaggag
     7621 gcgaggtttg aacgcgctgc gaattattcc ctgtctcgat gtaaaagatg gccgcgtcgt
     7681 gaaaggagtc aacttcgtga acctgcgcga tgccggtgat gccgttgagt gcgcgcaggc
     7741 ctacgaccg
//
LOCUS       DAOQJF010000139         7704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5170_length_7704_cov_5.811217, whole genome shotgun sequence.
ACCESSION   DAOQJF010000139 DAOQJF010000000
VERSION     DAOQJF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5170_length_7704_cov_5.811217"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..883)
                     /locus_tag="PLF85_08675"
     CDS             complement(110..883)
                     /locus_tag="PLF85_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNE19780.1"
                     /translation="MKIISFNVNGIRAIEKKGELQNLINKEKPDLLFLQEIKAKPEQL
                     SNDLLNPQGYEAHYHSAEKPGYSGTGVFIRQGLFDKAHVLRGMPEWNDHEGRVLGVQS
                     GNLTIFGNYFPNGGKSPAAWKEKLQFYDHFHQYIASLRKKKQRIIFTGDLNVAHNPID
                     LARPKENEKSVGFLPEERAWVDRLIADNWVDVFRSRYPETVSYTWWQMQSRARERNIG
                     WRIDYFMVDKPLYGAVENVRHLNDHMGSDHCPVVLEINL"
     gene            1007..1918
                     /locus_tag="PLF85_08680"
     CDS             1007..1918
                     /locus_tag="PLF85_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19781.1"
                     /translation="MSLFGLLLGLSTALSAQFLTPEETVKLPNPAEVPAAPATPVRPE
                     PAPAPAQAMPVPQSTPSADADAYFSDFAAYGREKNIILIWHLARGRTIERRIQIYRFT
                     EEPRVIHDISRGTLIAKLSGEINLYEDLPPAKGTYYYAIFVESVRGLEPASFNISRNL
                     VGPVAFNSATAATTTSAASKEPVQQTQKYPRPEFESEEVDEESEAIPAAVETSAEEKH
                     TRGINAAIRRTFFKGEYQQAVRELKPFLRNSSARVRAKAMFYTGLSRYRLGQYDRALK
                     YFDHPLTKKFYRRNAEFWIRKTTENLR"
     gene            1920..3131
                     /locus_tag="PLF85_08685"
     CDS             1920..3131
                     /locus_tag="PLF85_08685"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19782.1"
                     /translation="MRSVTVWIGAIVFTIGAGVFLIEREYLSGDKIARVRLEQGKLQL
                     ERFDEDGLRKGIDELTAVVANYPESIYAREARFHLAEAYERLGMRDVALGKYKRLAEM
                     PVSDQLKARVRFKIAKLQIMRNYSDEGMNQLLSLLNSTSEKTLRSEIYLEIGKYYQRK
                     GLAKDARRNYENALTENPENREARAALGMTDADYTNSVALHGILDKQEAPVTEKKEVA
                     AEQKFDLNPGIKAFTQGDYARAIHLLLPAAKSTAPESEEALYYVGNAYLKQKEYRNAV
                     HYLNKAVGNSYRGRDEASYIKKGEAYYLNNQLERAAHVFQFVRTRYPNGKYAQIAADW
                     ENETRRLLGDKTGTKSLPDDDDDGDDDDDVLGSVAVGDEAAPRPKKSGKVAKAESSEE
                     LTLDDDADVTP"
     gene            complement(3128..4516)
                     /gene="dnaA"
                     /locus_tag="PLF85_08690"
     CDS             complement(3128..4516)
                     /gene="dnaA"
                     /locus_tag="PLF85_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNE19783.1"
                     /translation="MLHTEQTAHQPVQELLLAGKTDYWATIKERVQQKIPAQVFAIFF
                     AELEARWENNTLFIICPSAEVKKHLQGQYRRLIAEEIKALGLSAEIQLLDNAQVTREN
                     PPVVQQKTVARAETAFPNKIDLNPNYTFDRFIKGPSNEHAYAAALGAAEKPNNYHNPL
                     YIYGGVGLGKTHLMMAIGNYVATNYPWLKVKYCPSEIFQSDLIEAMKDKNLSYFRTRY
                     RSVDVFLLDDVQFISKNADFTQEEIFHTFNYFYQNKKQIVISADRPPQALPQLHDRLQ
                     SRFQQGLIVDVKAPNFETRMAILKTKAQESGIQISADVIKYLATRFTSQVRPMEAALS
                     ILQFTSQHEKRPIDLQMAKMSLKALPQENGIHQVTIEDILRVVSRTLHVDESLIVGSN
                     RTEQVALARHVCMYLAKQLIQGATLSYIASAFGKRDHTTVLHADQKVRELVDTDMALQ
                     MQIEEMLEELKF"
     gene            complement(4711..5151)
                     /locus_tag="PLF85_08695"
     CDS             complement(4711..5151)
                     /locus_tag="PLF85_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNE19784.1"
                     /translation="MLDFEVFTSTLRNVFLQFIGIKDLTSENLPKLPDKFQSVTARIE
                     LSGTASGTLWLNISHPGVVLAMQRLNIPNSDEDRMMMDAAGELLNIIVGAAQRNSPVR
                     YDFSLPSAYKGDAYPLRFAENSRKSARRFIYDEFEAILILEQDN"
     gene            complement(5138..5521)
                     /locus_tag="PLF85_08700"
     CDS             complement(5138..5521)
                     /locus_tag="PLF85_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE19785.1"
                     /translation="MQLLIVDDSELMRTIVEQVIRENLQEAAAKIDFFHAANGKDAMD
                     IFQKNDIRLIFLDWNMPVLDGVEFVREIRGTGNKVPIVMITSVTDSERILQAAAAGVN
                     SYVEKPIRGPQLWDQIAMFFKNAGF"
     gene            complement(5524..>7704)
                     /locus_tag="PLF85_08705"
     CDS             complement(5524..>7704)
                     /locus_tag="PLF85_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNE19786.1"
                     /translation="GVTLLTPADIKYLQTLDAELNSAQHAPVLPPGVTGTVLSALAED
                     EKTAIIRTTAYQIIVQKLRRLRFASGKMAAHETILPAERHSEGNESQIHRAWIAGVRM
                     HDITPMHLRVLAADEFEEIDRDHNGRIDPHEKLYRIGDIFGGKNQHGVAAALKGEVSV
                     GNGYRSESSGVFIAGYTPILDSMGRVIALLVIDFSAATEFDALFRLKVTGYYIIVGVL
                     LLSIIAASLTSKLLLKPLEAMQKAAVRIGQRDFSIRIETGSSDELSDLAYAMNLMAQE
                     LGEYSTQMERRIAARTREISGILGALEQGVLTIDRNGIIQSEYSAATLAIFGMKEIAH
                     RRFSELFEDEKLSSAVDRYVEVYFSGKDISAPMLEKANPLRQITYRNKQGDIRHLRFS
                     FRPLAGDVDGEIHRLLVTVVDETYGVALQEKISLAEADKRSEFDAIINMMRVPAPILE
                     AFMTQQKEFLRDGKHLIGAFGSDRNLLTAFASRTHALKGNALQLGFIPLAERLHELED
                     YLAQASGGSEGDARFLRHEISRCVNSCEGLILGRDKLVTRIRELVGETDQQSETMGLD
                     QLRLFWLNQINRKAAEYSVPAAVEVSFAPGSEQAVHELHNVIVQLLRNTFAHGLEDTS
                     ERSFRGKPQPLHISLVAVVSGDQVELTYREDGRGIAGLPLGLTVPVQEMLQKGLTSSH
                     KSATLEAGRGLGMEYIASSVAALRGKVTVNTSERETIFRIVVPT"
BASE COUNT         1783 a         2010 c         2024 g         1887 t
ORIGIN      
        1 ctatccgtat ggttctgcag gatttggtgg tttgcgagga ttgtgggggg aagatctgcc
       61 cacagttttc tgacgattcc tgcagctccc ggttgcaggc ttccccgtct cagagattga
      121 tttcgagtac tacagggcag tggtctgagc ccatgtgatc gttcaggtgc cgcacgttct
      181 ccactgcgcc atacagcggc ttatcaacca taaaataatc gatcctccac ccgatattgc
      241 gttcccgtgc gcgcgactgc atctgccacc aggtgtagct cacggtctca gggtagcgcg
      301 aacgaaacac atccacccag ttatcggcga ttagccggtc gacccaggca cgctcctccg
      361 gcagaaaacc gacgcttttt tcgttctctt tggggcgtgc aagatcaatc gggttgtgag
      421 ccacgttcag gtcacccgta aagatgatgc gttgcttctt tttgcgcagc gaagcaatgt
      481 actggtggaa atgatcgtaa aactgcaatt tttccttcca tgcggcgggg gatttacctc
      541 cgtttgggaa gtaattgccg aagatggtga gattgcccga ttgcacgccg agcacgcgtc
      601 cttcatggtc attccactca ggcatgccgc gtagaacatg cgccttatca aacagcccct
      661 gccgaataaa cactcctgtt cccgaatatc ccggcttttc agcgctatgg tagtgcgctt
      721 catatccctg aggattcagc aggtcgttgc tgagctgctc gggttttgct ttgatctcct
      781 ggagaaacag caggtcgggt ttttctttgt taatcaggtt ctgcaattca cctttcttct
      841 caatcgcccg aatcccgttc acgttgaagg aaatgatttt catcgacccg atagtatctg
      901 cgtctacctc aggcggaaaa taacttaatc atgtctcata ttacagcttc cggatgccga
      961 agtttaaatg tcaggttctg tcatgctacg cccgtgcggt aatattatgt ctctgttcgg
     1021 ccttttgctg ggtctcagca ccgcgttatc cgcacaattt ctgactccgg aagagacggt
     1081 gaaattacct aaccccgccg aagtacctgc ggcaccagct accccggtgc gccccgagcc
     1141 cgctccggcc cctgcgcaag ccatgcctgt gccacagagc acgccttccg ccgatgcgga
     1201 tgcatatttc agcgattttg cggcctacgg ccgcgagaaa aacatcatcc tgatctggca
     1261 tctcgcacgc ggccgtacca tcgagcgtcg catacaaatc tatcgtttta cagaagaacc
     1321 aagggtgatc catgatattt cccgtggtac cctgatagct aaactctcgg gggagatcaa
     1381 tctttatgaa gacttgcccc cggctaaggg gacctattat tacgcgatat tcgttgagag
     1441 cgtaaggggc cttgaaccgg catcctttaa tatctcccgc aatctggtag gcccggtggc
     1501 attcaactca gccactgcag ctacgaccac ctcagcagca tcgaaagagc cggtacagca
     1561 aacccagaaa tatccgcggc cggaattcga gtctgaagaa gtcgacgaag aaagtgaagc
     1621 tataccagct gcggtagaaa ccagcgcaga agagaaacac acaaggggca tcaacgccgc
     1681 tatccggcgc acttttttca agggtgaata ccagcaggca gttcgtgaac tcaagccgtt
     1741 cctacgtaat tcatccgcgc gtgtcagggc aaaagcgatg ttctatacag gcctttcgcg
     1801 ctatcgtctc gggcagtacg atcgtgcgct caaatatttt gaccacccgc tcaccaagaa
     1861 attttaccgc cgcaacgcgg aattctggat caggaaaaca acggagaact tgaggtaata
     1921 tgcgcagtgt aacagtctgg attggagcga tcgtatttac gatcggggcc ggcgtctttc
     1981 tcattgagcg cgagtacctt tcgggagaca aaatagcgcg cgttaggctt gagcagggta
     2041 agctgcaact tgagcgcttc gacgaagacg ggctgcggaa gggtattgat gaactaacgg
     2101 ctgtcgttgc gaattatcct gaaagtatct atgcacgtga ggcacgcttt catcttgcag
     2161 aggcctatga acgtcttggc atgcgtgacg tagcgcttgg caaatacaag cgccttgctg
     2221 aaatgcccgt ttctgatcaa cttaaggcgc gcgtgcgctt caagatcgca aaactgcaga
     2281 tcatgcgcaa ttattctgat gaaggaatga accaactact cagtctgctg aactcgacca
     2341 gcgaaaagac gctgcgttct gaaatttatc ttgagattgg taaatattac cagcgcaaag
     2401 gcctggcgaa agatgcccgg cgcaactatg aaaatgcact gacggaaaat cctgagaacc
     2461 gcgaagcacg ggctgcgctg ggtatgacag acgccgacta tacgaactcc gtcgcgctgc
     2521 acggtatact cgacaagcag gaagcaccgg taacagaaaa gaaagaggta gcggccgagc
     2581 agaaatttga cctgaatcct ggcattaaag cattcacgca gggtgattat gcacgtgcga
     2641 tccacctctt gctcccagcg gctaaatcga cagcccccga gtcagaagag gcgctttact
     2701 atgtcggtaa cgcttatctg aagcagaaag agtatcgcaa tgcagtacac tacctgaaca
     2761 aggcagtggg gaattcgtac cggggccgcg atgaggcctc gtatatcaag aaaggtgaag
     2821 cttactacct gaacaaccag ctcgagcgtg ccgcccatgt ctttcagttt gtccgtacgc
     2881 ggtacccaaa cggcaagtac gcgcagattg cggctgactg ggaaaacgaa acccgccgcc
     2941 tgctcggtga caagaccggt acaaaaagtc tgcctgacga tgatgatgac ggagacgacg
     3001 atgacgacgt actcggttca gtagcagtgg gcgacgaagc cgcgccccgt cctaaaaaga
     3061 gcggtaaggt tgccaaggcc gagtcctcag aggagctgac gctcgacgac gacgcggatg
     3121 taacccccta gaacttaagt tcttcgagca tttcttcaat ctgcatctgc agcgccatat
     3181 cggtatctac cagttcgcga actttctgat ccgcatgtaa aaccgtggtg tggtcgcgtt
     3241 tcccgaaagc actggcaatg tagctgagcg tggctccctg aatcagctgt tttgccagat
     3301 acatgcagac atgccgggca agcgcgacct gttcggtacg gttagaaccg acaatcagcg
     3361 actcatcgac gtgcagcgtg cgtgatacaa cgcgcagaat gtcttcaata gttacctggt
     3421 gtatgccgtt ttcctgtggt agcgccttca acgacatttt ggccatttga aggtcaatcg
     3481 gccgtttttc gtgctgcgaa gtgaattgca ggatactgag agcggcttcc attgggcgca
     3541 cctgcgacgt aaaacgtgtt gccaggtatt tgatcacgtc tgcactaatc tgtattcctg
     3601 attcctgcgc cttcgtttta agaatggcca tacgcgtttc aaagttgggc gcctttacat
     3661 cgacaatcag gccctgctga aaacggctct gcaggcggtc atgcagctgg ggcagggcct
     3721 gcggtggccg gtctgccgag atcacgatct gctttttatt ctggtaaaaa taattaaaag
     3781 tatgaaagat ctcttcttgt gtaaaatctg cattcttgct gataaactga acatcgtcga
     3841 gcaggaaaac atcaacgctg cggtagcgag ttctgaagta ggaaagattc ttgtccttca
     3901 tcgcctcgat cagatcactc tggaagattt ctgacgggca atatttcact ttcagccacg
     3961 ggtaatttgt cgccacgtag tttccgattg ccatcatcag gtgcgttttc cccaaaccca
     4021 cgccgccgta gatatagagc ggattatggt agttgttggg tttctctgcc gcaccgagtg
     4081 ccgcggcata cgcgtgctcg ttcgacggac ctttgataaa ccggtcgaac gtgtagttgg
     4141 ggttgagatc aatcttgttt ggaaacgcag tctctgctcg tgccacggtc ttttgttgca
     4201 caaccggggg attttccctc gtcacttgcg cgttatcgag cagctgtatt tctgcgctga
     4261 gaccgagcgc cttgatctcc tctgcaatga gccggcggta ctggccttgc aggtgtttct
     4321 ttacttcagc actgggacag atgatgaaca gtgtgttgtt ttcccaacgc gcctcgagct
     4381 ccgcgaagaa gatggcaaat acctgtgctg gtattttttg ctgcactctt tctttgatag
     4441 ttgcccagta gtctgttttg ccggctaaga gcagttcttg tacgggctgg tgggcggtct
     4501 gttcggtgtg gagcatgcgg aaaaatctgg aaacactgct gcaaattaca agcaaaggtt
     4561 ttcccggcaa cagggctttc gcatacaaat gaaggccaga aggtgtatag tttagaaaca
     4621 ggtattatgt cacataacag tacagaaaat gcgaaagcac acactgttct gccgctcgcc
     4681 aggtaatcct aacgaatctc gttcatccaa tcagttgtct tgctcaagaa tgagaattgc
     4741 ctcgaattca tcgtagataa atcgccgggc cgacttgcgc gaattttctg caaaacgcag
     4801 tgggtaggca tcacctttgt aagctgaagg cagcgaaaag tcgtaacgga ccggggagtt
     4861 gcgttgtgcc gcaccaacga tgatattcag cagctcgccg gccgcgtcca tcatcatgcg
     4921 gtcttcgtcg ctgttgggaa tattcagtcg ctgcatcgcc aggacgacgc ccgggtgcga
     4981 gatgttgagc caaagtgtac ctgaggcagt gcccgacagt tcaatgcggg ccgtcacgct
     5041 ctggaatttg tcgggcagct tcggcaggtt ttcgctggtc aggtccttga tcccgataaa
     5101 ctgcaggaac acattccgca gtgtagatgt gaaaacttca aaatccagca tttttgaaga
     5161 acatcgcaat ctggtcccac aactgcggtc ccctgatggg tttttctacg tagctgttaa
     5221 ctcctgcagc agctgcctgc aggatgcgct cactgtcggt caccgaagtg atcatgacaa
     5281 tcggcacctt gttaccagta cctcttattt cgcggacaaa ttcaacgcca tccagcacgg
     5341 gcatgttcca atcgagaaaa atcaggcgaa tatcgttctt ttggaatata tccatggcgt
     5401 ctttgccgtt tgctgcgtga aaaaaatcaa ttttagcagc agcttcctgc aggttctcgc
     5461 gaatcacctg ctcaacgatg gtacgcatca attcagaatc gtcgactatg agcagctgca
     5521 ttttcaggtt ggtaccacga tgcgaaatat cgtttctctc tcactcgtat taacagtcac
     5581 tttaccgcgc aaggcagcaa cgctcgaggc aatgtattcc atacccaagc cgcgccctgc
     5641 ttcaagcgtc gcagacttat gggaggacgt caatcccttt tggagcattt cctgcacggg
     5701 cactgtcagt cctagtggca gaccagcaat gccccttccg tcttcgcggt acgtcagttc
     5761 gacttgatcg ccggatacta cagcaactag tgaaatgtgc agcggctgtg gttttccgcg
     5821 aaaactgcgt tcgctggtgt cttcgagacc atgtgcaaaa gtgttacgca gcaattgaac
     5881 aatcacgttg tggagttcat gcacggcctg ctccgagccg ggagcgaagg acacttcgac
     5941 agccgcgggt accgaatatt ctgcggcctt ccggttaatc tgattcaacc agaaaagccg
     6001 tagttggtca agtcccattg tctctgactg ttggtctgtt tcgccgacga gctcgcgtat
     6061 ccgtgtaacc agtttatcgc gtcccaggat gagtccttcg cacgagttta cgcaacgcga
     6121 tatctcatgt cgcagaaacc gcgcgtcacc ttcactgccg ccacttgcct gcgcaaggta
     6181 atcttccagt tcgtgcaatc tttccgcgag ggggataaaa ccaagctgca gcgcattgcc
     6241 tttgagtgca tgggtccggg aggcgaatgc agtcaggaga ttgcggtcgc tgccgaaggc
     6301 gccaatgagg tgttttccgt cgcgcaggaa ctctttctgc tgcgtcatga acgcctcgag
     6361 tatcggcgcg ggcacgcgca tcatgttgat gatcgcgtcg aactcagacc gtttgtctgc
     6421 ctctgcgagg gatatttttt cctgcagcgc gacaccgtat gtttcatcca ccacagttac
     6481 cagcaaccga tgaatttctc cgtcgacatc accagccagc gggcggaacg agaatcgcag
     6541 gtggcgaatg tcgccctgtt tgtttcggta agtgatctgc cgcagaggat ttgccttttc
     6601 cagcataggg gcgctgatat ctttgccgga aaagtagact tcgacatagc gatcaacagc
     6661 cgagctgagc ttttcatctt cgaagagttc agaaaaacgg cgatgcgcaa tttctttcat
     6721 accaaaaatc gcgagcgtcg ctgcggagta ttcggactgt atgatgccgt tacggtcaat
     6781 ggtgagtact ccctgttcca gtgctccgag aatgcctgaa atttcgcgcg tgcgtgcggc
     6841 tatacggcgc tccatctggg tcgaatattc acccagctcc tgggccataa gattcatcgc
     6901 gtaggccagg tctgacagtt cgtcgctgct gcctgtttca atgcgaatgg aaaaatcacg
     6961 ctggccgatg cgtacggctg ccttctgcat ggcttcaaga ggcttcagta gtagtttgct
     7021 tgtcagtgaa gcagcaatga tcgataataa cagaacgccg acgattatat aatagccagt
     7081 gactttcagg cgaaaaagcg catcgaattc tgttgccgcc gaaaaatcaa tcacgagcaa
     7141 cgcgataaca cggcccatgg aatcaagtat tggggtgtag cctgctatga atacgccgga
     7201 actctcggaa cggtagccgt tacctacaga aacttcgcct ttgagtgcgg cggcaacgcc
     7261 atgctggttc ttgccgccga aaatatcgcc tatccggtac aatttttcat gagggtcgat
     7321 gcgaccatta tggtcgcggt cgatttcctc gaattcatcc gctgcgagca cccgcaagtg
     7381 cataggagta atgtcatgca tcctgacgcc ggcgatccat gcccggtgta tctgggactc
     7441 gttgccttct gagtggcgtt ctgcgggaag gatcgtctca tgtgcggcca ttttgcccga
     7501 ggcaaaacgt agtctgcgca gtttctgcac aatgatctgg taggcagttg ttcggataat
     7561 tgcggttttt tcgtcttcgg ccagcgctga aagtacggtt cccgtcacac cgggcggcag
     7621 cacaggtgcg tgttgtgcgg agttgagctc ggcatcgagg gtttgcaggt acttgatatc
     7681 tgccggcgtc agaagggtta ctcc
//
LOCUS       DAOQJF010000140         7674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5199_length_7674_cov_4.802993, whole genome shotgun sequence.
ACCESSION   DAOQJF010000140 DAOQJF010000000
VERSION     DAOQJF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5199_length_7674_cov_4.802993"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLF85_08710"
     CDS             complement(<1..404)
                     /locus_tag="PLF85_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19787.1"
                     /translation="MENDAGKAAPTYEEWVALVGQDKTDKLYAGIGQDSLNLLAYGIG
                     QSNMVQLINGITEGAKLVTLVGNDGIAGTGPGSASGLGAVVTVYLLKSVDTQMQANIS
                     SNLVGGGVAPTDQDTVTNLVNVINGLTATEIQ"
     gene            787..1707
                     /locus_tag="PLF85_08715"
     CDS             787..1707
                     /locus_tag="PLF85_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNE19788.1"
                     /translation="MKFAVSSLIATSLLLSPLFAGSGSTSKPLAGSYPIVLSHGILGF
                     DDSLGLAGGLIKYWGGMDEHLRSQGAAVLTPGTNPVQSVVNRANDQKNLINMWMAANG
                     YTKVHIIAHSQGALVSRYMISSLGMASKVSTMTSINGVNRGTPVADFTLAVIPSWLQP
                     TVNVLVNTLSKLVYGKTQQDLKAMTASLTVSGVNALNAANPNMTGVKYYSYGSRVTLP
                     DLIQHPLMGLVFPITWTGGLFNGQGGENDGVVPLSSQKWGTWMGGPSYSILTTGVDHI
                     QATNFSYMGQPWYDVKAYYTKMATNAKNNQ"
     assembly_gap    1758..1857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1858..2042
                     /locus_tag="PLF85_08720"
     CDS             <1858..2042
                     /locus_tag="PLF85_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19789.1"
                     /translation="DAHFDRLSATKQKFTVTLSRSKGGSTRTSTGSVRRKQSSVILSL
                     SKGDATIYKSPEVPVN"
     gene            complement(2063..2614)
                     /locus_tag="PLF85_08725"
     CDS             complement(2063..2614)
                     /locus_tag="PLF85_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19790.1"
                     /translation="MRYVACLLLLASLAGPVTAAAPARFVPPEAAQPAPAWQLAAADF
                     LLPGYGTFRHGETGYAAFYFTSNLVNLSLVYLAWRNWRFYESAYDAASVRQATEPDTL
                     YFRDPTGGSDYLSLQDIRNRAERGQLFFAVSIAANVALRLLSATHTWNLADAARRQAG
                     PRYEFYPDTSGGFQARGGYYFYF"
     gene            complement(2621..3445)
                     /locus_tag="PLF85_08730"
     CDS             complement(2621..3445)
                     /locus_tag="PLF85_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE19791.1"
                     /translation="MSAFGATLQRSVNAFFKRYLPNAGFTKFLLGVSEYYWFSRQVFL
                     SGLQLRGKHLDTFVRVVINQVRFTGSQALPMLSLIALSIGGTTIIQATTFLPKLGQDN
                     FIGNLLKLVIVREVGPLITAIIVLARSGSAITAEMCTQKMHREIDAMEMMGINPHLIM
                     VLPRLAGGVISVVLLIIYFDVIAIMGGYLISMTVTSFPFSTFVDHLARSISMTDLLST
                     LMKSILSGIVIPLTCTYYGFKPQSLFQIPIFVSKAVIRSLFIVFILNAFVSVLFYL"
     gene            complement(3442..4281)
                     /locus_tag="PLF85_08735"
     CDS             complement(3442..4281)
                     /locus_tag="PLF85_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE19792.1"
                     /translation="MKIHHLQTGTFCPFGGPLMHDRGIGGGEAHLLCHSLLVETDDGL
                     VLIDTGFGTEDIENPHERLGTAFVTLVNAQLKPELTAVRQIEALGFSRNDVRHIIPTH
                     LDLDHVGGLPDFPRARVHVYEDEYLAAMQPRTFNEKQRYRAAHWRHGVDWQRYTLEGE
                     AWYGFPAVRSLLGAGKDEILIVPVTGHTRGHSAIAVHGENGWLLHCGDAYFHKGQVDI
                     AHPHCPVGLEVFQRLMAVDNDARAENVERLRTLRMEHGHDVTLFSAHDKDEFEQLAAK
                     AAV"
     gene            4377..6065
                     /locus_tag="PLF85_08740"
     CDS             4377..6065
                     /locus_tag="PLF85_08740"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE19793.1"
                     /translation="MEMQFSRTSLLSRAAVYLAFITVHLAATLMAPYYFSSVGLVTLF
                     WPATGIALAAVLAGGYGYAWATLISCTIAGVLDPGKGWAQAFFTAANVIEILIARLIV
                     LRLARVDIAFDKSPDYIRFIIFAGVLLPVPAALIAATTLHFVSQSATPFWFNAQEWWM
                     SDSLGILSLTPLLLIWRRLPQGWFYRERLLEAILGLALTVVASYLFLGTPGESAASYP
                     RAFLFFIFVAWAATRFGRHATLLVTTIVIAFALAGSTAAGKTPDGRAINFANIWLFMV
                     TLLTVGMALATVFNERRAALQKNAQLLEAYRSEESRRRAADQAARKSAIDFERLVETA
                     SEGVWAIDSGGVTTFVNSRMAEMLGYNPADMLGKSFFDFMRSSELDFGRRQLERRNAG
                     ITEGHEGILLHKDGHPIDVWMSTSPITDIEGNVNGGLALVTDISSRKQIELQLRECEE
                     RYDKIVNCISDAVLIHQSGIILFANPAAAELLGEKNAASLVGMNSLNYAHPDEQPVII
                     ERMKQLLHSEQKEFLPPIRQRMVLRNGRVIMIETITGALPFKGRTALVVIAKRV"
     gene            complement(6066..6902)
                     /locus_tag="PLF85_08745"
     CDS             complement(6066..6902)
                     /locus_tag="PLF85_08745"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNE19794.1"
                     /translation="MPTALFEKVRARSTLFRPSLPSKDDAGRFIAELLDLLFAGSHLT
                     QQEDPAAWIAELATLQETLRQLLLPFVESDNRAREISSDFFQAQLPVIYEVLWQDAEA
                     AYAGDPAAQNLQEVVLAYPGFYGVAAYRIAHYLHSLAIPTLPRLLSEHAHEKTGIDIH
                     PGAKIGRSFFMDHGTGIVIGGTSVIADNVKIYQGVTLGALSVDKSLASTKRHPTIEEG
                     VVIYAGATILGGDTIIGRNSVIGGNTWIVESVLPYSVVYNKSSSRVRPAKEMSDVIDF
                     SI"
     gene            complement(6902..7087)
                     /locus_tag="PLF85_08750"
     CDS             complement(6902..7087)
                     /locus_tag="PLF85_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19795.1"
                     /translation="MARRLANKGRRNLPKTTELGRRSFSSTEARIHEYKVRIRDRDYL
                     EFAIDRIAIELTHFLTK"
     gene            7329..>7674
                     /locus_tag="PLF85_08755"
     CDS             7329..>7674
                     /locus_tag="PLF85_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19796.1"
                     /translation="MRNDFALDPVLTNKDDSDPILEYKNIDTSSLPARKVLLGKIPFY
                     QATSADLVAHSIHTLDLMREKKSKSTGQFGPQLVFPFDPYRYVWVRFRKRMLEMANQS
                     FINLPDGSGMLYM"
BASE COUNT         1751 a         2063 c         2087 g         1673 t
ORIGIN      
        1 tgctgaattt cggtcgctgt cagaccgttg atcacgttca ccaggttcgt cacagtgtcc
       61 tgatcggttg gcgcgacgcc accaccgaca aggttagaag aaatgttagc ctgcatctga
      121 gtgtcgacag acttgagcag gtagacagtc acgactgcac cgagacccga ggccgagccc
      181 ggccctgtac cagcgatgcc gtcgtttcca accagcgtca ccagttttgc accttctgta
      241 ataccgttaa tgagctgcac catgtttgac tggccgatgc cgtaggcgag caggttcagg
      301 ctatcctgac caatgccggc atacagtttg tcggttttgt cctggccaac cagcgctacc
      361 cattcttcat aggtgggcgc ggctttaccg gcgtcgtttt ccatattttg cgcgaatttg
      421 gtctttgatg accttgcgca attcagcgcg aaagcagccg ctactaccag tagcagaccg
      481 accttcaggt gcctcttaaa tttgctcatt gttgtctcct gcagcatttg tcgcttgtgg
      541 gtttagacgt agaccggggt aaacgcacga cattcgttta ctctgcgcat gcgcataccc
      601 cgggccacac tagccctgta tacaactata tatccatcgg aagtttagag tgccaaaaaa
      661 agtaaacaag tgtaaatgat ttgtgcggtg ctgaagaatt gcattttttt ggcaccgcaa
      721 acagctcggc ggcctttact agttacccac gggtatatcc tgaatcagtt cgtcagcgga
      781 gaattcatga aatttgcagt ttcctcatta atagcgacat cactccttct ttcaccccta
      841 tttgccgggt cgggcagcac ctcgaaaccg ctcgctggca gctaccccat cgtgctttcg
      901 catggtattc ttggcttcga tgatagcctg ggattggcgg gtggcctcat taaatactgg
      961 ggaggaatgg atgaacacct gcgcagccag ggagcagccg tgctgacgcc gggcacgaac
     1021 ccggtccagt cggtggtgaa ccgcgcaaac gaccagaaaa acctgatcaa catgtggatg
     1081 gcagccaatg gctacacaaa agtgcatatt attgcgcact cgcagggagc gctggtgtct
     1141 cgctacatga tcagctcgct gggtatggcg tcgaaagttt cgacgatgac ttccatcaat
     1201 ggcgttaatc gcggcacgcc tgtggcggat tttacccttg ctgtgatccc gtcgtggttg
     1261 cagccaaccg tcaatgtact ggttaacaca ctctcgaagc tggtctacgg taagacacag
     1321 caagacctca aggcgatgac tgcctcgctc accgtgtcag gcgtgaacgc gctcaatgcg
     1381 gcgaacccga acatgaccgg ggtgaagtac tactcctacg gttcgcgggt caccctgccc
     1441 gacctgatac agcatccgct gatgggcctg gttttcccga tcacctggac gggcgggctc
     1501 tttaacggcc agggcggcga gaacgacggc gttgtgccgc tgtcttcgca gaagtggggc
     1561 acctggatgg gaggtccctc ctacagtatt ctgaccacgg gtgtagacca catacaggcg
     1621 acgaactttt cttacatggg ccagccatgg tacgacgtga aggcgtacta tacaaagatg
     1681 gcgacgaacg cgaagaataa tcaataaatt tccgtcaccc tgagcccgtc gaagggtgac
     1741 acgtcgcgtg cttcgacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg
     1861 acgcgcactt cgacaggctc agtgcgacga aacaaaaatt caccgtcacc ctgagtcgat
     1921 cgaagggtgg ctcgacgcgc acttcgacag gctcagtgcg acgaaaacaa tccagcgtca
     1981 tcctgagcct atcgaagggt gacgcgacga tatacaaatc acccgaagtg cccgttaatt
     2041 aattctttag cgagcgggat cactaaaaat aaaaatagta cccaccccgc gcctggaagc
     2101 caccactcgt atcgggataa aattcatagc gcgggcctgc ctgccggcgt gctgcgtcgg
     2161 caagattcca ggtatgcgtc gcgctgagca ggcgtaacgc aacatttgcc gcaatcgata
     2221 cagcgaaaaa caactgcccg cgttctgcgc ggttacgaat atcctgcagt gaaaggtagt
     2281 cgctgccacc agtgggatct ctgaagtaca gggtatcggg ctctgtggcc tggcgcacag
     2341 atgcggcgtc ataggcagat tcatagaagc gccagttgcg ccaggcaagg tagacgagtg
     2401 aaagattcac gaggttgctg gtgaaataga acgccgcata accggtttcg ccgtgccgaa
     2461 aggtgccata acccggcaga agaaaatcgg cggcagccag ctgccaggca ggtgccggtt
     2521 gtgctgcttc gggtggcaca aaacgggcag gcgctgcagc tgtaaccggc cctgcgagtg
     2581 aagcgagaag cagcaggcag gcaacgtaac gcatgagctc ctacaggtaa aaaagtaccg
     2641 agacgaaggc gttcaggata aagacgataa acaacgagcg aatcactgcc ttggaaacaa
     2701 agatcggtat ctggaacagg gactgtggtt tgaagccgta gtaggtacac gtcagcggaa
     2761 tcacgattcc cgagagtata gatttcatca gcgttgacaa caagtcagtc atggaaatcg
     2821 agcgggccag gtgatcgaca aaagtcgaaa agggaaacga ggtgaccgtc atagagatca
     2881 ggtagcctcc catgatggca atcacgtcga agtaaataat cagcagtacg acagagatca
     2941 cgccaccggc gaggcgcggc agaaccatga tcagatgtgg attgataccc atcatctcca
     3001 tcgcgtcgat ttcgcggtgc attttctgtg tgcacatttc agccgtgatc gcagaccccg
     3061 aacgtgcaag cacaataatc gcggtaatga gcggccctac ttcgcgcaca atgacgagct
     3121 tgagcaggtt accgatgaag ttatcctggc caagcttggg caaaaaggtg gtcgcctgga
     3181 taatcgtggt gccaccaatc gagagggcaa tcagggagag catcggcaat gcctgcgagc
     3241 ccgtgaagcg cacctggttg atgacaacgc ggacaaacgt atcgaggtgt tttccgcgca
     3301 gctgcaggcc agacaggaac acctggcggg agaaccagta gtactcgctg actcccagaa
     3361 ggaatttcgt gaaccccgca ttcggcaggt aacgtttgaa gaaggcgttt accgaacgct
     3421 ggagtgtggc gccgaaagca ctcatactgc tgctttagcg gcgagctgtt caaattcgtc
     3481 tttgtcgtgc gcactaaaca gtgtcacatc gtgcccgtgt tccatacgca gcgtacgcag
     3541 gcgctcgaca ttttctgccc gcgcatcgtt atctaccgcc atgagccgct ggaatacttc
     3601 gaggccgacc gggcaatgcg gatgcgcgat gtcaacctgg cctttgtgaa agtacgcgtc
     3661 accgcagtgc aagagccagc cattttcacc gtgcactgca atcgccgaat gcccgcgtgt
     3721 gtgacccgtg acgggtacaa tcagaatttc gtctttgccg gcgccgagca gcgatcgtac
     3781 agcgggaaaa ccataccacg cttcgccttc gagtgtatac cgctgccaat ccacgccgtg
     3841 ccgccagtgc gctgcgcggt agcgctgttt ctcattgaat gtgcgcggtt gcattgcggc
     3901 aaggtattca tcttcgtaga cgtgcaccct ggcacgggga aaatccggca ggccgccgac
     3961 gtggtcgaga tccaggtgcg tcggtataat gtgccgcaca tcgttgcggc tgaagcccag
     4021 cgcctctatc tgccgcaccg cggtgagttc gggcttcagc tgcgcattta ccagggtgac
     4081 aaaagcagtg ccgagacgtt catgcgggtt ttcgatgtct tcggtgccaa aaccggtatc
     4141 aatgagaacg agcccgtcgt cggtttcgac gagcagcgaa tggcaaagca ggtgcgcctc
     4201 gccgccgccg atgccgcggt catgcatcag tggccccccg aaggggcaaa aggtgcccgt
     4261 ctgcaggtgg tgaattttca tcgggtcaga ctgactaccc aatcatgccc ggcaagcaat
     4321 tcggcaccgc cgaaactttt gcctttactt agcaggctaa gccggcaagt gggtgcatgg
     4381 aaatgcagtt cagccgtact tcgctgctgt cgcgcgcggc ggtctacctg gccttcatca
     4441 ccgtacatct cgcggccacc ctcatggctc cctattattt ttcttctgtt ggcctcgtta
     4501 cgcttttttg gccggcaacc ggcattgcgc tggctgcggt actggccggc ggttatggtt
     4561 acgcctgggc aactctgatc tcgtgcacaa tagcaggtgt gctcgacccc ggcaagggct
     4621 gggcgcaggc atttttcact gcagccaatg tcattgagat cctgattgcc cgtctgattg
     4681 ttttgcgact cgcacgggta gatattgcat tcgacaagtc gcccgactac atcaggttta
     4741 ttatttttgc cggagtactg ttgccagttc cggctgcgct gatagctgca accacgctcc
     4801 attttgtttc gcaaagtgcg acaccgtttt ggtttaatgc gcaggaatgg tggatgagcg
     4861 actctcttgg tattctttcg ctgaccccgc tgctgcttat ctggcgcagg ctaccgcagg
     4921 gctggttcta ccgcgaacgc ctgctagaag ccatactggg acttgcgctg accgtggtcg
     4981 caagctacct ttttttgggc acaccaggcg aaagcgcagc gtcatatcca cgggcattct
     5041 tattcttcat ttttgttgcc tgggcagcca cgcgctttgg ccggcatgcc accctgctgg
     5101 ttaccaccat agtcattgcg tttgcgctgg cgggtagcac agcagcgggc aagacacccg
     5161 atggccgtgc gataaacttt gcgaacatct ggctgtttat ggtgacactg ttgacagtcg
     5221 gcatggcact tgccacggtc tttaacgagc ggcgggcagc cctgcaaaag aacgcgcaac
     5281 tgcttgaagc ataccgcagc gaagaatcac ggcggcgcgc cgcagaccag gcagcccgca
     5341 agagtgcgat tgacttcgag cgacttgtgg aaactgcgag cgaaggtgtc tgggcaattg
     5401 actctggagg agtaactaca tttgtgaaca gccgcatggc tgaaatgctg ggctacaacc
     5461 cggcagatat gctgggaaaa tcattctttg actttatgcg ctcctctgag ctggactttg
     5521 gccgcaggca actcgaaagg cgcaatgccg gtattaccga aggacacgaa ggtatactgc
     5581 tgcataaaga cgggcacccc attgatgtct ggatgagcac gagcccgatt acggatatag
     5641 aaggcaatgt caatgggggt ttggccctgg tgaccgatat ttcgtcgcgc aaacagattg
     5701 aattacagtt gcgtgaatgc gaagagcgct atgacaaaat tgtgaactgc atttctgatg
     5761 cagtcctgat acaccagtca ggcatcatcc tgttcgccaa ccctgccgca gccgaactgc
     5821 tcggagagaa gaacgcggca tcgcttgtgg gcatgaattc cctgaactat gcgcacccgg
     5881 acgaacagcc agtcatcatt gaacgcatga agcagctact gcacagtgaa cagaaagaat
     5941 tcctgccacc catcaggcag cgtatggttc tcagaaacgg gcgggtaatc atgattgaga
     6001 caatcaccgg tgccctgcct ttcaaaggca gaaccgcttt ggtcgtcatt gccaaaagag
     6061 tctgatcaga tagaaaaatc gatcacgtca gacatttctt tggccggcct gacgcgactc
     6121 gagctcttgt tgtaaactac tgaatagggc agcacacttt cgacaatcca ggtattgccg
     6181 ccaataaccg agttgcgacc gatgatagta tcgcccccca gaatcgtagc cccggcgtag
     6241 atcacaacac cttcttcgat tgtaggatgt ctctttgtac tggccaggct tttgtcaacg
     6301 gagagagccc ccagtgtgac accctggtaa attttgacgt tatcggcgat caccgaagtt
     6361 ccaccgatga caatacccgt accgtggtcc atgaaaaacg accggccgat ttttgccccg
     6421 ggatgaatat caattccagt tttttcgtgc gcgtgttcgc tgagtagtct cggcagcgtt
     6481 ggtatggcga ggctgtgcaa atagtgagca atgcggtaag ctgccacacc gtaaaagccc
     6541 ggataagcca ggacaacttc ctgcaggttc tgtgctgccg ggtcgcctgc atacgcggcc
     6601 tcggcatctt gccagagtac ttcatagatg acgggcagct gtgcctgaaa aaaatcggaa
     6661 ctgatttccc gtgcccggtt atctgattcg acaaacggca gcaacagctg ccgcagtgtt
     6721 tcctgcagag tcgcgagctc ggcaatccag gcagccgggt cttcctgctg ggtcaggtga
     6781 ctgccagcaa aaagcaggtc aagcagttcg gcaatgaacc gccctgcgtc gtccttggac
     6841 ggaagcgacg gccgaaagag cgtgctgcgc gcacggacct tttcaaaaag tgccgtaggc
     6901 attacttcgt gagaaagtgc gttaactcga tcgctatccg gtcgatggcg aattcgaggt
     6961 agtcacggtc ccgtatgcgg accttgtatt catgaatacg tgcttcagta gaagaaaacg
     7021 accgccggcc gagttctgta gttttgggca aattacgcct gcccttattc gcgagtctgc
     7081 gagccatagt tacttccttg taacgtgttt gccagccagg ccccataggg acatggctgg
     7141 gccattgtca ggccatcctt ggcctcagga ttatcatcgt cgccggccga aaaatgctaa
     7201 agcatttttc ggccgggggg acttaagtat tgacaggctg ttccgacggc cggccttcaa
     7261 acaggcgaca taatcatgga cttttcaaga gcagccagcg aagaatcttc cttacatgag
     7321 gtagcccggt gagaaacgat ttcgccctcg accccgtatt aaccaataaa gacgattcgg
     7381 atcccatcct cgagtacaag aacattgata cgtcatcttt gccggcgcgc aaagtactgc
     7441 tcggcaagat acctttttat caggcgacct cggccgatct cgtcgcccac tcgatacaca
     7501 cactcgacct gatgcgggaa aaaaagagta aatccaccgg ccagtttggt ccgcagctcg
     7561 tgttcccatt cgatccatac cgctacgtct gggtcaggtt ccgcaagcgc atgctcgaaa
     7621 tggccaacca gtcattcatc aacttacccg atggttctgg catgctgtac atgt
//
LOCUS       DAOQJF010000141         7664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5214_length_7664_cov_4.701800, whole genome shotgun sequence.
ACCESSION   DAOQJF010000141 DAOQJF010000000
VERSION     DAOQJF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5214_length_7664_cov_4.701800"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLF85_08760"
     CDS             complement(<1..472)
                     /locus_tag="PLF85_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE19797.1"
                     /translation="MRWGALHVLLACGLLQAAAFASQAETEFRQALRLHNANDLRAAA
                     QTYRRALELDPTLVSAAINLAIIHEKWGESDTAEKLYDSAVRAAPNLFSSRYNRGQFL
                     QKHGRLAEARADYQIALEQKPDEASLYINLAAIEIRLFEMTRDVALLSAAELRLK"
     gene            complement(523..1206)
                     /locus_tag="PLF85_08765"
     CDS             complement(523..1206)
                     /locus_tag="PLF85_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19798.1"
                     /translation="MSTGEIRSSNILRIGSSTLTELEEKVKALRTINLNVKKRRFVIA
                     REDIKHPSGEILVPKGSDIDISKVMLLKRHFKPDHLIKTFQPDEGIVLVSDMSTPVGI
                     QFSMDLVTQVMNIGGGAYEAFIDRVDSFKEFAALYNKALFPKLAIVGYIPMQPERIKE
                     ELAFYQLITRTDPYIRMIEILHSQIKPQPIVPRMRNLVISPEDRDSWKRFILEIIAEY
                     TKAYSIEER"
     gene            1305..>1953
                     /locus_tag="PLF85_08770"
     CDS             1305..>1953
                     /locus_tag="PLF85_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE19799.1"
                     /translation="MQNHFPPACHLGLVPALQFSAFLTDSLSQIGAAGIFLLRSCRGI
                     VQRPFRAAKISFEIYTIGWQSLAIILFTGAFTGMVLGLQGYHVLKRFGADGFLGSLIA
                     KSLLAELSPVLSALLIIGRAGSALCAEIGVMRLSEQVEALECLAIDIYNYLVSPKLLA
                     MLISLPLLVFVFTVSGMLGGYFSGCIVLGVSVGDYYQSMRDMIDFATVRMAIIKSF"
     assembly_gap    1954..2053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2054..2275
                     /locus_tag="PLF85_08775"
     CDS             <2054..2275
                     /locus_tag="PLF85_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE19800.1"
                     /translation="MRDMIDFATVRMAIIKSFVFAVFIVTVTAFRGLYVHQYSGRGAL
                     ALSRTTTLAVVVASVGVLLLDYLLTAVLI"
     gene            2275..3024
                     /locus_tag="PLF85_08780"
     CDS             2275..3024
                     /locus_tag="PLF85_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE19801.1"
                     /translation="MARPTSRPEIAIELTGISKQLGGLPVHTDLNLKIEKGKTTVVVG
                     ASGSGKSVMLKYILGFMTPDAGDVRVYGKSILGLSETEMKKIRSLYGVVFQGAALFDS
                     LNVFDNVALPLVEKTKKTRAEIEDQVMHYLAEMRIADARHKMPAELSGGMQRRVALAR
                     ALQLNPEIVLFDEPTTGLDPETSENIYDLFIETQQRLKYTALIVSHDIPRIFRVGSWI
                     AVLNGGKIESVIDSKHAVKKAGGWLRKMLDV"
     gene            3069..3503
                     /locus_tag="PLF85_08785"
     CDS             3069..3503
                     /locus_tag="PLF85_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNE19802.1"
                     /translation="MRRTEIYTGLFIVFGLFCFAWVALSLGGLRMFGDSRHVYYAEFQ
                     STSGLKPGAIVEIAGVQVGNATALDLKDNRARLVLRIERSVKIPVDSIAAIRTRGLIG
                     EKFVKITPGGDDELLQPGGEISETESVVDLEEMIGKFIYDKK"
     gene            3500..3790
                     /gene="hisE"
                     /locus_tag="PLF85_08790"
     CDS             3500..3790
                     /gene="hisE"
                     /locus_tag="PLF85_08790"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNE19803.1"
                     /translation="MNVQFLAELENLLKDRRQNMPEGSYTTKLYTAGPQKILQKVGEE
                     AIEYILDAQNADKQKAVYEGADLLYHFMVSLVAQGWSLGDIIAELEKRHKKP"
     gene            3810..4952
                     /locus_tag="PLF85_08795"
     CDS             3810..4952
                     /locus_tag="PLF85_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19804.1"
                     /translation="MVTGLAAQAPAPANAAEKAKTPPAPLTAPATKATPPDNKTPATE
                     SGANTAPAANAEAKPKPASPELIARIRDALKFGNSQQVRDALNTLGRLTADEQKTLLP
                     ELKQTCKSQDGLVLRKMAEFIGNAPFNDLDDELAQFLTDKTHEQLFFATVGAIAKKKP
                     ASALPVLTKEIREQDFSKPGNRIPDAVHLLTIYKDNSLQNFLVEKLHAADTYADYRSG
                     ILKYLGEATPWSPQLRDRVLKLFQDEAEPLTVRGSSAYALGKAQIAEAKPVLKEALAK
                     IENMKSVDEKKRYTRFRMQVIASLILLKDNDVREILYAMARDDDEQVRLRAVHQIGQL
                     KIEDARQLLTYKSKFDPSARVQKEAKKALSLLDGQKVVPDEPGKEP"
     gene            4949..5392
                     /locus_tag="PLF85_08800"
     CDS             4949..5392
                     /locus_tag="PLF85_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19805.1"
                     /translation="MKYAAIIVLVSAMVLPAGVHSFFAPGDQTLEQAVAAGAPVDLFL
                     KYQAHEGDYLVFYDATGSILLVRYRRDRWDYDQDTLRDSLRQGITYRIRCRNLNKLPE
                     GEVPAGVMGAGLPRVTTIRKIRRIRDLYAGELEAVTESALRDLRY"
     gene            5394..6626
                     /locus_tag="PLF85_08805"
     CDS             5394..6626
                     /locus_tag="PLF85_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNE19806.1"
                     /translation="MLLFLLLLLTLLSGFYSGSEAAIFSQDAGRLARMRSDEFSPRLR
                     KTVIGWLRRPERIITGLLLGNLIVNIGMTNIWETWIVGEFGEFTHRHIVLPVVITLYV
                     LTFGEVIPKIIALVFKDTWVKALQLPLRGWFRFSARFTSPFDRLTARLVKPLKPVSST
                     LTEAELVEAVRFAEDHGLLKSEEMRMLSRSIAFYHNTVYAAMIPRSQILLLPEGTSLS
                     AARKAFLASAHNFAAIYRRNSTEIEGVIYLRGVVQLQLARKKNLENKVHPVEFLPASL
                     SLSAALSALMNNRRDIAAVVDEAGAFIGMVTLRGIINHILGASFSPVPQDTYLEQLDN
                     RRYRVAAQMPLDRFNEIFRTNLTAELSETIGGYLLEHLDGFPHGDEEIPIGNLTFRNF
                     DLEEHRIRSFVLVVKRNG"
     gene            6619..>7664
                     /locus_tag="PLF85_08810"
     CDS             6619..>7664
                     /locus_tag="PLF85_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNE19807.1"
                     /translation="MVSVAIFIFCLISAAFFAATEVAFFSLTPLTVARGNRDRLEKLL
                     LNKNDIIAMLLTGNNLAIVCGTLALDALIPPGRDIWYKLGAFFTELVLFFLLSEALPK
                     AIGRRKNILLLEKSYSLIWLFYYLLLPMSYVFLRISRLIGKVSVSHPDDARVEVFRFI
                     SEHTSDSKLPLAESLATYSSTTIREIMTPMSELFSLGSSARLSECAETIERSSYSRYP
                     VYDAIPADIIGYLDMRDLLDKPGSTRVRQVMRPAHFFPYSLTVDQLHAEMRRLAVPMV
                     FVVNEYGLILGMVTEENLAEELVGDIFSHDQKLETQYLVEKEGGRFEIDCVMDIDDFI
                     KVFGAQISKEHFETV"
BASE COUNT         1675 a         2040 c         2138 g         1711 t
ORIGIN      
        1 tctttaaccg tagctccgcc gcagaaagga gcgcgacgtc acgcgtcatt tcaaacagcc
       61 gtatttcaat agcagcgagg ttgatgtaca gcgaggcctc atcgggcttc tgttccagcg
      121 caatctggta gtcggcgcgt gcttctgcga gcctaccgtg tttctgcaga aactgcccgc
      181 ggttatagcg cgaggagaaa aggttggggg ctgcgcgcac cgccgagtca tagagttttt
      241 cggcagtatc actctcgccc catttttcat ggatgatcgc caggttaatt gccgcagaaa
      301 cgagcgtggg atcaagctcg agcgcgcgac ggtaggtctg tgcagcggcg cgcaggtcat
      361 tcgcgttatg taacctgagc gcctgcctga attccgtttc tgcctgcgaa gcaaaagccg
      421 cagcctgcag tagtccacag gccagcagaa cgtgcagagc tccccaccgc atctgtttaa
      481 atgtcggcgg aaaacggcag atgttaagtc aaaagcgggt tgttaacgct cttcgatcga
      541 gtacgctttc gtgtattcag caataatttc gagaataaag cgcttccacg aatcgcggtc
      601 ttcaggactg atcaccaggt tgcgcatacg cggtacaatc ggctgcggct tgatctgcga
      661 gtgcagaatt tcgatcatgc ggatgtaggg gtcggtgcgc gtgatgagct ggtagaaggc
      721 cagctcttct ttaatacgtt ctggctgcat cgggatataa ccgacgatgg ccagcttggg
      781 aaaaagagcc ttgttataga gtgcagcgaa ttctttgaag gaatcgacgc ggtcgataaa
      841 tgcctcgtag gcgccgccgc cgatattcat cacctgcgtg accaggtcca tggagaactg
      901 tatacccaca ggggtactca tgtccgagac caggacgatg ccttcgtcgg gttggaaggt
      961 cttgatcagg tgatcgggtt tgaagtgccg cttgagcagc atcaccttcg agatatcaat
     1021 atcgctgccc ttcgggacga gtatctcgcc ggaagggtgt ttgatatctt cgcgcgcaat
     1081 aacgaaccgg cgcttcttta cgttgaggtt gatcgtccgc agcgccttga ctttttcttc
     1141 gagctcagtc agcgtactgg agccaatgcg caatatattg gacgatcgaa tttcgccggt
     1201 cgacatggag tgcaataccc gcatgtccgc gcgcggtgtc aatcaatgcg ctcggataaa
     1261 tatccttggc cctgccatgg aaaacaggcg cgagtcggtc cggcatgcaa aatcactttc
     1321 cacccgcgtg tcaccttggg ctcgtacccg cgttgcagtt ctcggccttc ctcactgatt
     1381 cattatccca gatcggcgct gccggcattt ttttgctgcg ctcgtgccgt ggtattgtgc
     1441 agcgcccttt ccgcgcagca aaaatttctt ttgaaatcta cacgatcggc tggcaatcgc
     1501 tggcgatcat cctctttaca ggggcattta ccggcatggt ccttggcctg cagggttacc
     1561 atgtcctcaa gcgctttggt gccgacggtt ttcttggttc gctgatcgcg aaaagccttt
     1621 tggcagaact ttctcctgtt ttgtcagcgt tgctgattat cgggcgcgcc ggctctgcgc
     1681 tctgtgcaga gatcggtgtc atgaggctgt cagagcaggt tgaggcgctc gagtgcctgg
     1741 cgatcgatat ctataactac ctggtttcgc caaagcttct cgcgatgctg atttcgctac
     1801 cgctgctggt ttttgttttt actgtcagcg gcatgctcgg tggctacttc tcgggttgca
     1861 tcgtgctagg tgtcagcgtg ggcgactact accagagtat gcgcgacatg atcgactttg
     1921 cgaccgtgcg catggcaatc atcaaatcgt ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnatgcgcg acatgatcga ctttgcgacc gtgcgcatgg caatcatcaa
     2101 atcgtttgtt tttgcagttt ttattgtgac ggtcacagca tttcgcgggc tttacgtgca
     2161 ccagtattcg gggcggggag ctcttgcctt gtcgcgcact acgacgctcg cagtggtcgt
     2221 cgcctcagtg ggtgtgctgc tgctcgatta cctgctcacg gcggtgctga tctgatggca
     2281 cgcccgacca gcaggcctga gattgcgatt gagcttacgg gcatcagcaa acagctcggt
     2341 gggctgccgg tgcatactga cctcaatctg aaaatagaaa aaggtaagac cacagttgtg
     2401 gtaggggcca gcggttcagg caagagcgtc atgctcaagt acatcctggg ttttatgacc
     2461 cccgacgccg gtgacgtcag ggtttatgga aagagcattc tcggtttgag cgaaacagaa
     2521 atgaaaaaaa tccgttcgct ttacggcgtg gtcttccagg gggcggccct tttcgattcg
     2581 ctgaacgtct ttgataacgt cgcattgccc ctggtggaaa agaccaaaaa gacgcgcgcc
     2641 gaaatcgaag accaggtgat gcactacctc gcggagatgc gcatcgcgga cgcccggcac
     2701 aagatgcctg ccgaactctc ggggggtatg cagcgccgtg ttgcgctggc gcgcgcgttg
     2761 cagctgaatc ccgaaatagt gctcttcgac gaacccacaa cgggtcttga tcccgaaacc
     2821 agtgagaaca tctacgacct gttcatagaa acgcagcagc ggctgaagta cacggcgctg
     2881 attgttagcc acgatatacc ccggatcttt cgagtgggca gctggatcgc ggttttgaat
     2941 ggcggaaaaa ttgagtcagt gatcgactcg aagcatgcag tgaagaaagc cggcggttgg
     3001 ctgaggaaga tgttggatgt ctgacaggac gactaaaaag gggctcaagc ccaagcggag
     3061 tttagaaaat gagacggact gaaatttata ccgggctttt catcgtgttt ggcctgttct
     3121 gtttcgcctg ggtggcgctg tcgctgggtg ggctgcgcat gttcggcgac agccggcatg
     3181 tctactacgc tgaattccag tcgacttcgg ggctgaagcc cggcgccatt gtcgagattg
     3241 ccggcgtgca ggtcggtaat gctaccgcgc tcgatctcaa agacaatcgg gcgcggttgg
     3301 tgttgcgcat agagcgcagc gtcaagattc ccgtcgattc gattgcggcg attcgcacgc
     3361 gtgggctcat cggcgagaaa tttgttaaga ttaccccggg gggggacgac gaactgttgc
     3421 agccgggcgg agaaatcagc gaaactgaat cggtggtcga tctcgaagaa atgatcggca
     3481 aattcatcta cgacaaaaaa tgaacgttca attccttgca gaactcgaaa acctgctgaa
     3541 agaccgcagg cagaatatgc ccgaaggctc gtataccacg aagctctata cagccggccc
     3601 gcagaagata ctgcaaaagg tcggcgaaga ggcgattgag tatattttag atgcccaaaa
     3661 tgccgataaa cagaaggcag tatatgaagg cgccgacttg ttataccact tcatggtctc
     3721 cctcgtggcg cagggctggt cactgggtga tatcatcgcc gagctggaga agcggcataa
     3781 gaaaccttag tctcggacca ttattgttgg tggtaacggg gctcgcggcc caggcgccgg
     3841 ccccggcaaa tgccgccgaa aaagcgaaaa ctcccccagc gccgctaacg gcacctgcaa
     3901 cgaaagccac cccaccggat aacaagacgc cggcgacaga atctggcgca aacacagcgc
     3961 ccgctgccaa cgcagaggcc aaaccgaaac cggcatcacc cgagctgatt gcgcgaatcc
     4021 gcgatgcgct taaatttggc aattcacagc aggtgcgcga tgcactgaat acgctggggc
     4081 ggttgactgc cgacgagcaa aagacgctgc tgcccgaatt aaaacagacc tgcaaatcgc
     4141 aggatgggct tgtactgcgc aagatggcgg agttcattgg caatgcgccg tttaacgatc
     4201 tcgacgatga acttgcgcaa tttctgaccg ataaaaccca cgagcagctg ttctttgcca
     4261 cagtgggagc tattgcgaaa aaaaagcctg cctctgcttt gccggtgctc accaaagaga
     4321 tacgcgaaca ggacttttca aagcccggta accgtatacc cgacgcggtg cacctgctca
     4381 cgatctacaa agataattcg ctgcagaatt tcctcgtcga gaaattgcac gcggcagaca
     4441 cctacgcgga ttaccgcagt ggcattttga aatacctcgg cgaggcgacg ccctggtcgc
     4501 cacagttgag agaccgtgtc ctgaaacttt tccaggacga agcggagccg ctgactgtac
     4561 gcggatcttc tgcgtatgca ctcggcaagg cgcagattgc cgaggcaaag cccgtgctca
     4621 aagaggcgct ggcaaaaatt gaaaacatga agagcgtcga tgaaaagaaa cgctacactc
     4681 gctttcgtat gcaggttatc gcgagcctga ttctgctgaa agataacgac gtacgggaaa
     4741 ttctctacgc catggcgcgc gatgacgacg aacaggtgcg cctgcgcgca gtgcaccaga
     4801 taggccagct gaaaattgaa gatgcacggc agcttctgac atacaaatcc aaatttgacc
     4861 cgtcggcgcg ggtacagaaa gaagcaaaaa aggcgctcag cctgcttgat ggccagaaag
     4921 ttgtgcccga cgaacctggt aaagaaccgt gaaatacgcg gcgatcatcg tgcttgtctc
     4981 tgcgatggtt ttaccggcag gagtgcattc cttttttgcg cccggcgacc agacgctgga
     5041 gcaggctgtg gctgccggtg cccccgttga cctgttcctg aaataccagg ctcatgaggg
     5101 tgattacctt gtcttttacg atgcgacagg ctcaattctg cttgtccgct accgccgcga
     5161 ccggtgggat tacgaccagg acacgctgcg cgattcgctg cggcagggta tcacctaccg
     5221 catccgctgc cgaaatttga acaagctgcc cgaaggcgaa gtgccggcag gtgtcatggg
     5281 ggcaggtctg ccccgcgtaa cgacgatccg caagattcgc cgtatacgcg atctctacgc
     5341 gggcgaactc gaagcagtta cagaatcggc gctgcgcgac ctgcgttact gagatgctgc
     5401 ttttcctcct tttgcttctg accttactga gcggcttcta ttcgggcagc gaagcggcga
     5461 tcttctcgca ggatgccgga cggcttgccc gcatgcgctc cgacgagttc tcaccgcgcc
     5521 tgcgcaaaac agtcattggc tggttgcgtc gccccgagcg cattattacc gggctgctgc
     5581 tcggcaacct gatcgtcaac atcggcatga ccaatatctg ggagacatgg atcgtcggcg
     5641 aattcggcga gtttacccac cgccacatcg tgctgccggt cgtgatcacg ctctacgtcc
     5701 tgactttcgg cgaggtgata cccaagatta tcgcgctcgt attcaaagat acctgggtaa
     5761 aggcgctgca gctgccgctc aggggttggt tccggttttc cgcacgtttt acaagcccgt
     5821 tcgaccgact gacagcacgc cttgtcaaac ccctgaagcc agtcagcagt acgctgactg
     5881 aagccgagct tgtcgaagcc gtgcgctttg ccgaggacca tggcctgctc aagagcgaag
     5941 aaatgcgtat gctctcgcgt tcgatcgctt tttaccacaa caccgtttac gcggcgatga
     6001 tcccgcggtc gcagattctg cttctgccag aaggaacctc gctctccgcg gcgcgcaagg
     6061 cattcctggc atcggcgcac aatttcgcgg cgatctaccg cagaaactcc accgagattg
     6121 aaggcgtgat ctacctgagg ggagttgtgc aattacagct ggcgcgcaaa aaaaatctgg
     6181 agaacaaggt gcaccccgtg gaatttctgc cggcaagcct gtcgctgagt gcagcgcttt
     6241 ctgcgctgat gaacaaccgc cgtgatatcg ccgcggtggt cgatgaagcc ggcgctttta
     6301 tcggcatggt cacgctgcgg ggcatcatca accacatact gggagccagc ttttcgccgg
     6361 tcccccagga tacttacctc gaacagctgg ataaccgtcg ctaccgcgtc gccgcgcaga
     6421 tgccgctcga ccggtttaac gagattttcc ggacgaacct cacagccgaa ctgagcgaaa
     6481 ccataggtgg ttacctgctc gagcatctcg acggttttcc gcacggcgat gaagaaatcc
     6541 cgatcggaaa cctgacgttt cgcaacttcg acctcgaaga gcaccgcatc cgtagttttg
     6601 tcctggtggt aaaacggaat ggttagcgtc gctatcttta tcttctgcct gatctccgct
     6661 gcgttcttcg cagctacaga ggtcgcattc tttagcctta ccccgctgac agttgcgcgg
     6721 ggcaaccgcg accggctgga gaagctactt cttaacaaga atgacatcat tgctatgctg
     6781 ctcaccggca ataacctcgc cattgtctgc ggtacactgg ctctggacgc gctgataccg
     6841 cctggcaggg atatctggta taagcttggg gcatttttta ccgagctcgt gcttttcttt
     6901 ctgctctcag aagcattacc caaggctatt gggcgtcgca agaatatcct gctgctcgaa
     6961 aagagttata gcctgatctg gttgttctac tacctcttgt tgccgatgag ttatgtgttc
     7021 ctgcgtatct cgcgcctgat tggcaaggtg agcgtcagcc accccgacga tgccagggtt
     7081 gaggtattcc gtttcatttc ggagcatacc agcgatagta aattgccact cgcagagtcg
     7141 ctggcgacct attcgagcac aaccattcgt gagattatga cacccatgag tgagttgttt
     7201 tcgctcggct cgtcggcccg gctcagtgaa tgcgccgaga cgatcgagcg cagttcttac
     7261 tcccggtacc ctgtctacga tgcgataccc gcagatatca tcggctacct ggacatgcgc
     7321 gacctgctcg ataagccagg tagtacccgc gtgcggcagg tcatgcggcc cgcgcacttt
     7381 ttcccgtact cgctgacggt cgaccagcta cacgccgaaa tgcgcaggct tgcggtgcca
     7441 atggtctttg ttgtcaatga atacgggctg atactgggaa tggtgaccga agagaacctg
     7501 gcagaagagc tggtcggtga tatcttcagc catgaccaga agctcgaaac gcagtacctg
     7561 gtagaaaaag aaggtgggcg ttttgaaata gattgcgtga tggatatcga cgacttcatc
     7621 aaggtatttg gagcgcagat cagtaaagag cactttgaaa cagt
//
LOCUS       DAOQJF010000142         7634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5238_length_7634_cov_5.511545, whole genome shotgun sequence.
ACCESSION   DAOQJF010000142 DAOQJF010000000
VERSION     DAOQJF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5238_length_7634_cov_5.511545"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1221
                     /locus_tag="PLF85_08815"
     CDS             166..1221
                     /locus_tag="PLF85_08815"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19808.1"
                     /translation="MATVRRADHKLTALCRKIQSMPAVPRFVIFVADQNEDLAELLST
                     LREKLVTSFGSCDEVHLSGYDNDAATWHAEMMTMPMFPSGRLILIRHAEALLKRIDGQ
                     ARVLANYLHDFPQAPEFTVSVLQFGEKKIPKKLQPLEELALVYEDQPLSPEELADHLS
                     ARAQGLGFTAERDALDLLIDKCAGKERLVLAGFDRLITFRLHEKKISVADVEEVIGTA
                     ESNMHFRLLDETARRNVGECLRILQLHALESAEQLIAALARLFSEAMRYHYYERSGMS
                     AAEIGQVIAQRPLTGYPLKKSIERWSLLLQKYSPAGIRLVMDALVKADILIKENSDAG
                     QQQVILTSFYLMLSKAL"
     gene            complement(1230..1868)
                     /locus_tag="PLF85_08820"
     CDS             complement(1230..1868)
                     /locus_tag="PLF85_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE19809.1"
                     /translation="MSAELVKRFGSTYSAGQTIFQEGDHAEEMFIIHQGQVNISKRAR
                     NAEQVLATLRDGDFFGEMALFTDSPRSATATVASESVILRIDKKSFDYMINNNSSFAI
                     NMIRKLCERLRNADNQISELLVFSRETRVLKAMAGYWNVEGHKDASGQVLLLPYLGFC
                     EYLKKTAGITIEDANQSLLKLRNQNLLHIRKDIAGKQYISFSPKIFEYFNIV"
     gene            complement(1882..2106)
                     /locus_tag="PLF85_08825"
     CDS             complement(1882..2106)
                     /locus_tag="PLF85_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HNE19810.1"
                     /translation="MENKILIDIEKGRGEVIRVEVGEFKGKKLLHLRTWYTNDAGELA
                     PTKKGVALSYEQFQKLKSILPQVDDIMVGQ"
     gene            complement(2176..3207)
                     /locus_tag="PLF85_08830"
     CDS             complement(2176..3207)
                     /locus_tag="PLF85_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19811.1"
                     /translation="MPPADAVHIPRRWRRPRFWLKLGALLFAAAFTATTIWFILRAEQ
                     FILSRLPREIQASSISVSFLGRSFVLKGVRVSGRMGSPCEGRLLFEIAELTGQFVIRE
                     RRLTSLAVNGAELINQGWQKSCFAATPESRPLRLSEITPASGLEISFRQVRFRLPYLG
                     EASATGSIRLSEPQPDGLQLRAAQLSLSGVRIQGGARELAVNLNRTNGNWQLATGNFT
                     ATLSLKQLEKIPKLSSRRLTVLAGNADIRLAATARQGNWKILTNVELSKVRLRGEPFY
                     TMPMGLLQLTPENVWPMVEDSPGLLSFNFETKAHQGQLVKEFTADLRRALTTKVKGNL
                     KKKVPVLPF"
     gene            complement(3207..3539)
                     /locus_tag="PLF85_08835"
     CDS             complement(3207..3539)
                     /locus_tag="PLF85_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNE19812.1"
                     /translation="MENCLFCKIVEKKIPAQVIFEDDDTLAFRDINPVAPVHILLVPK
                     KHLENILGLDAALAQAMHASLKKITEQEKLAQGFRVVANTGEHGGQTVYHLHWHILSG
                     RHMGWPPG"
     gene            complement(3588..5114)
                     /locus_tag="PLF85_08840"
     CDS             complement(3588..5114)
                     /locus_tag="PLF85_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19813.1"
                     /translation="MICSRRLKKKRGSGAEPPASLLLRQIFPAVLLVLLSQACSSKSV
                     KTPTQTAGVEASTSEEVSGTADIAQPPERAESPPLEPLRKAPPKLDKKVKKKRGRFQF
                     SGDEFIKESGTNPFEKKNDTIIKLRGHARISARNVKMSSPMIEIYGDDGHLAYARGPV
                     EIVDTRNATRITADEALFIRSENRAVLRGNARLVTQVKDKKNTQEKIQLTSQELERNF
                     ETSVSLARGNVVATGRSAILYANSAEFLETQELVLSEDSPRIFSDSDVFLADKIQWDL
                     AKNTSDFRGNVRAYFSRPDNDDRRKRPVESAVRAEEGTLQKDDSLPFGQKLTLRRRVS
                     LDRKTYSAYSDAAEVFGQGAELVRASDNVTLINREENTRSYGDTFEWTKATGAMALSG
                     RKQNRTRTIFYNKKSVPTAEIAATTVTKAKEKANPQARGDVSIMQFSSDSSAPPVKMG
                     SEWAEVQRDRKVILLHGSPYVEGEMGRIGAREIILHYEEQRYEMLGILPGMVEKRMGP
                     "
     gene            complement(5104..6453)
                     /locus_tag="PLF85_08845"
     CDS             complement(5104..6453)
                     /locus_tag="PLF85_08845"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE19814.1"
                     /translation="MAEGVTEQGLQSHRWPPVHKGKLERPHIIIVGGGFAGLTLARRL
                     KRAPVYITLIDKANHHLFQPLLYQVATASLSPGHIAMPLRAIFRGQKNVRVLMNRVGE
                     VRAAEKKIRLVTGDEFSYDHLVLATGARHSYFAHPEWEKTAPGLKSLADALRIRQKIL
                     ASFEKAEAMLAIGNADPAELSKLLTFVVVGGGPTGVELAGSIAEIAHQTLRKEFRGID
                     PRTTRVILVEGGEHLLMAFPGSLGDNAKKELERLGVEVRVKATVGNVMYDGVQIGGGP
                     AGAAELVSTSNVFWAAGNAASTLLKTLGTPLDRAGRAIVQGNLSLEAYPEICVLGDAA
                     HCVDAATGNPLPGVAPVAIQQGQYLAKRIIAETKGKTVPDFHYWDKGSMATIGRGRAV
                     AKIGRVHVTGVIAWLMWSLIHVLYLVGFRNRFAVMILWMYSYITNRRAVRLITADDL"
     gene            complement(6454..7335)
                     /gene="mtnP"
                     /locus_tag="PLF85_08850"
     CDS             complement(6454..7335)
                     /gene="mtnP"
                     /locus_tag="PLF85_08850"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNE19815.1"
                     /translation="MKHKVDIGIIGGTGVYSVEGMKVIDKINVKTPWGKPSDAVTIAT
                     YEENGKSVTLAFLPRHGKGHFIPPTAIPAHANMAALKMLGCEAVIAFSAVGSLKEEIA
                     PGHFVLPDQIIDRTRHRRDTYYDEGIVVHVSFGDPMDANLAGILKEAIQKLQLPLHEK
                     ETLICMEGSQFSTRAESKLYKSWGAGIINMSVLPEAKLARELELPYQMICMATDYDSW
                     REHDHAVTADEIMAVVKKNSDNAQRVLKAALPLLAAKKGSLNSLRGSVKFGVITAPDK
                     RPARLRKKYNSVLPGTF"
     gene            complement(7332..>7634)
                     /locus_tag="PLF85_08855"
     CDS             complement(7332..>7634)
                     /locus_tag="PLF85_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HNE19816.1"
                     /translation="VEKAAAESREPVWALPIGPRYRELLNSDIADINNIGGRYGGACT
                     AAEFLNAFVDEGQMWVHLDIAGVGMIKKPFSVYTASGSGYGVRLLAEVARQMAGGV"
BASE COUNT         1594 a         2202 c         2063 g         1775 t
ORIGIN      
        1 ctgcgttact gcaccgataa cgcggcaatg gtggcagccg cggcgcacct ctactttgcc
       61 cgcggtatcg agtcgaagtt taccaacatc agctcttcga acagcttttc cggtgtggca
      121 ggtataacga cggccggtat gcccggttcg gggcagcggt aaacaatggc tactgtgcgc
      181 cgggcagatc ataaactcac ggcgctttgc cgtaagatac agtctatgcc ggcagtaccg
      241 cgcttcgtaa tttttgtcgc cgaccaaaat gaagatttgg cagaactact ttccaccctg
      301 cgtgaaaagc tcgtgaccag cttcggcagc tgcgacgaag tacacctttc aggctacgac
      361 aacgatgcgg caacctggca cgccgaaatg atgacgatgc ctatgttccc cagcggcagg
      421 ctgatactca tacgccatgc cgaggcgctg ctcaagcgta tcgacgggca ggcgcgcgta
      481 ctcgccaact acctgcatga ctttccgcag gcgcctgaat tcaccgtatc ggtgctgcag
      541 ttcggcgaaa agaaaatccc taagaaactg caaccgctcg aagaactggc gctcgtctac
      601 gaagaccaac cgctttcacc cgaagaactc gctgaccacc tgtcagcgcg cgcgcagggc
      661 ctcggcttca cagccgaacg cgatgctctc gacctgctga tcgacaagtg cgccggtaag
      721 gagcgtctcg tccttgccgg ttttgaccgt ctgatcactt tccggctgca tgagaagaag
      781 atctcggtgg ccgacgtcga agaggtgata ggtaccgcgg aaagtaacat gcacttcagg
      841 ttgctcgacg agactgcgcg ccgcaatgta ggtgaatgcc tgcgcatatt gcagctgcac
      901 gcactcgaga gcgccgaaca gctgattgct gcactggcgc gcctgttcag tgaggcgatg
      961 cgctaccact actacgaacg cagcgggatg tcggcagcgg aaattggcca ggtaattgcc
     1021 cagcgtccgc tcaccggtta cccgctgaaa aagagtatcg agcgctggtc actgttgctg
     1081 cagaagtatt ctccagccgg catacgcctg gtgatggatg cgctcgtgaa agcagatata
     1141 ctgataaaag agaactcgga tgccggccag caacaggtta ttctgacgag cttctacctg
     1201 atgctttcga aagcgctgtg agctgcccgt cagacaatat tgaaatattc gaatatcttc
     1261 ggtgaaaacg agatatactg cttgccggcg atatctttac gtatgtgcag aaggttctgg
     1321 ttacgcagtt tcagcaggct ctggttggca tcttcaatcg tgataccggc agtttttttc
     1381 aggtattcgc agaaaccgag gtaaggcagc agcaatacct gccccgaggc gtccttgtgg
     1441 ccctcgacat tccagtaacc ggccattgcc ttgagcacgc gtgtttcgcg gctgaagacg
     1501 aggagctcgc tgatctggtt atcggcgttg cgcagccgct cgcagagctt gcgtatcata
     1561 ttgatcgcaa acgagctgtt gttattgatc atgtaatcaa aggatttctt gtcgatgcgc
     1621 agtatgacac tttcgctggc gacggttgct gtcgccgaac gtggcgagtc ggtaaagagc
     1681 gccatctcgc cgaaaaaatc gccgtcgcgc agcgtagcca gcacctgctc ggcattgcgg
     1741 gcacgcttcg atatattcac ctgaccctgg tgaatgatga acatctcttc ggcgtgatca
     1801 ccttcctgga aaatggtctg gccggcgcta taggttgaac cgaaacgttt gacgagctct
     1861 gcactcatat tctttgggga ctcactgccc aaccattata tcatcgacct gcggcaaaat
     1921 gctcttaagt ttctggaatt gttcgtaact cagtgcgacg ccttttttgg tcggcgccag
     1981 ttcgccggcg tcattggtgt accatgtgcg caggtgcagg agcttcttgc ctttaaactc
     2041 ccccacttct accctgatca cttctccacg gcccttttca atatcgatga gaatcttgtt
     2101 ttccatggct tttgccagaa ccggctcgcc ggttgcgacg gataatcctt tttcggccgg
     2161 gccccccggt taagcctaaa aaggcagtac gggtactttc tttttgaggt tgcccttgac
     2221 cttggtcgta agcgcccgcc gcaggtctgc ggtgaattct ttgaccagct gcccctggtg
     2281 tgctttggtc tcaaagttaa aggacaacag gccgggagaa tcttctacca tgggccagac
     2341 attttcaggc gtcagctgca gcagtcccat gggcatggta tagaacggct cgccgcgcag
     2401 gcgcaccttg ctgagttcaa cattggtaag gattttccag ttgccctgcc gtgcagttgc
     2461 agccagccga atatctgcat tgcctgccaa aacagtcagg cgacgcgaac tcagcttggg
     2521 tatcttttcc agctgtttca ggctcagcgt cgccgtaaag ttacctgtgg ccagctgcca
     2581 gttgccgttg gtacgattga ggttcactgc cagctcacgt gccccaccct ggattcgtac
     2641 accgcttaac gacagctgcg ccgcccgtag ttgcagccca tccggctgcg gttcgctcag
     2701 ccgaattgat cctgttgccg aggcctcgcc gaggtatggc aggcgaaaac gcacctggcg
     2761 gaaactgatc tcgagcccag aagcgggtgt gatctccgac aggcgcaaag gccgcgattc
     2821 gggagtggca gcaaagcacg acttttgcca gccctggtta atgagctcag ctccgttgac
     2881 tgcaagcgag gtcaggcgcc gctcgcggat gacaaactgc ccggtcagct ccgcaatctc
     2941 aaacagcagc cggccttcgc agggggagcc catgcgtcct gaaaccctga cgcctttaag
     3001 aacaaagctc cgcccgagaa acgataccga tatactgctc gcctggattt cgcgcggcag
     3061 ccgcgagaga ataaactgtt ctgcacgcag aatgaaccag attgttgttg cggtgaatgc
     3121 tgcggcgaac agcaatgccc ccagttttag ccagaaccgc gggcgccgcc accgccgggg
     3181 gatatgaact gcgtctgctg gcggcattaa ccgggaggcc agcccatgtg acggccggaa
     3241 agaatatgcc agtgcaggtg gtagactgtc tgaccaccat gctcaccggt gttagccaca
     3301 acacgaaaac cctgggcaag tttctcctgc tcggtgatct tcttgagact ggcgtgcatg
     3361 gcctgtgcca gcgcggcatc gagcccgaga atattctcca ggtgtttttt aggcacaagc
     3421 aaaatgtgca cgggagcaac aggatttatg tcacggaaag cgagtgtgtc atcgtcttca
     3481 aatataacct gagccggaat cttcttttcg acgatcttac aaaacagaca gttttccatt
     3541 catcctccag tatagctatc gaacagcatc atcgtgccat tacccgatca ggggcccatt
     3601 cttttttcga ccatacctgg cagaatgcca agcatttcgt atcgctgctc ttcgtagtgc
     3661 aggataattt cccgcgcgcc aatgcgaccc atttcgccct cgacataggg gctgccgtgc
     3721 aacagaatta ccttgcgatc gcgctgcacc tctgcccatt cagagcccat ttttacaggc
     3781 ggagcagagc tgtcactgct gaactgcatg atactgacat cgccgcgcgc ctgcgggttc
     3841 gccttttctt ttgccttggt taccgtcgtc gccgcgatct cggcagtcgg cacggatttt
     3901 ttgttgtaga agattgttcg cgtgcggttc tgcttacggc ctgacaaggc catagcaccg
     3961 gtagccttcg tccactcgaa agtatcgccg tacgaacggg tattttcttc ccggttgata
     4021 agcgtgacat tgtcgctggc ccgcacgagt tcggccccct gaccaaatac ttcggctgca
     4081 tcactgtagg ctgaataggt tttgcggtcg agcgagacgc gacgccgcag tgtcagcttc
     4141 tggccaaacg gcaggctgtc gtctttctgc agagtaccct cttcggcccg cacggccgat
     4201 tcgaccggcc gcttacgccg gtcatcattg tcaggccggc tgaaatacgc gcgaacatta
     4261 cccctgaagt ccgaggtatt tttcgccagg tcccactgga tcttgtcggc gagaaatacg
     4321 tcagaatcgg agaatatgcg cgggctgtct tctgaaagta caagctcctg ggtctccagg
     4381 aattcggcgc tattggcgta gagtatcgcc gagcggcccg ttgcgacgac attgccgcgc
     4441 gccagagaaa ccgaggtttc aaagtttcgc tcaagctcct gcgaggtgag ctggattttt
     4501 tcctgtgtat tctttttatc tttgacctgc gtcaccagcc tggcattgcc ccgcagcact
     4561 gccctgttct ctgacctgat aaagagcgct tcatcagcgg tgatacgcgt cgcgttgcgg
     4621 gtgtcgacga tctcgacggg gccgcgcgcg taggcaaggt gcccgtcgtc accgtaaatt
     4681 tctatcatag gagaagacat cttcacattc ctggcagaga tgcgcgcatg gcctctgagc
     4741 tttataatcg tatcattctt tttttcaaac gggtttgtcc cgctctcttt gatgaattcg
     4801 tcgccgctaa actggaacct gccgcgtttc ttctttacct tcttgtccaa tttcggcggg
     4861 gctttgcgca agggttcaag tggtggcgac tctgcccgct caggcggttg cgcaatatcc
     4921 gcagtaccgc tgacttcttc tgacgtggac gcctcgacgc ctgctgtttg cgtgggagtt
     4981 ttgactgatt tgctactgca ggcctgcgac agcaatacca gcaagacagc agggaagatc
     5041 tgccttagta gcagcgaagc aggaggttcg gcacctgatc cccgcttctt tttgagacgt
     5101 ctgctacaga tcatctgccg tgatcagccg gacagcgcgg cggttagtga tgtagctgta
     5161 catccaaagg atcatcaccg caaaccggtt gcgaaaccct acgaggtaga gcacatggat
     5221 cagcgaccac atgagccagg ctataacgcc cgtgacatgc acgcgaccga tcttggcgac
     5281 ggcccggcca cggcctatcg tcgccatcga tcctttgtcc cagtaatgga agtcaggcac
     5341 agttttacct tttgtttcgg caatgatgcg cttcgcgagg tattgtccct gctgtatcgc
     5401 tacgggagcg acacccggca acgggttgcc ggttgcagca tcaacgcagt gcgcggcgtc
     5461 accgagtaca cagatttcgg gataagcctc gagcgaaaga ttgccctgca cgattgcccg
     5521 accagccctg tccagggggg taccaagagt cttcagcagg gtcgaagcag cgttgcccgc
     5581 cgcccagaaa acattggacg tcgaaacgag ttcggcagct cccgccgggc cgcccccgat
     5641 ctggacaccg tcatacatga cgttacccac ggtggccttc acgcgtactt caacgccgag
     5701 tcgttcgagt tctttcttcg cgttatcgcc cagcgaaccg ggaaaagcca tcagcaggtg
     5761 ctcgccgcct tcaaccagta taacgcgcgt agtacgcgga tcaatccccc tgaattcttt
     5821 tctcagtgtc tggtgcgcaa tttctgcaat agaaccggcg agttctacgc ccgtggggcc
     5881 gccaccgaca acaacgaagg taagcaactt gctgagttca gccggatctg cattaccgat
     5941 cgcgagcatc gcttcggctt tttcaaaact tgcgagaatt ttctgcctga tacgcagagc
     6001 gtcagcgagg cttttcagac ccggggcagt tttttcccat tcaggatggg caaagtacga
     6061 atgccgggct cccgtcgcga gcacgaggtg atcgtagctg aattcatcgc cggtaacgag
     6121 gcggattttt ttctctgctg cacgcacctc acctacccgg ttcatcagca cccggacatt
     6181 tttttgccca cggaagattg cgcgcagagg catggcaata tgccccggcg agagcgaggc
     6241 tgtcgctacc tggtaaagca gcggctggaa cagatggtgg ttagccttgt cgatcagcgt
     6301 gatatatacc ggggcgcgct tcaggcggcg agcaagagtc aggccggcaa agcccccacc
     6361 gactatgatg atgtgcgggc gctccagttt acccttgtgc accggcggcc agcggtgaga
     6421 ctgtaagcct tgttccgtta cgccttcagc catctagaat gtgcccggta agacgctgtt
     6481 atattttttg cgcaggcgcg cagggcgctt gtccggggcc gtgatcacgc caaacttcac
     6541 agatcccctg agcgaattga gactgccttt ctttgctgcc agcaaaggca aagccgcttt
     6601 cagaacccgc tgcgcattgt ccgagttctt tttgaccact gccataattt catctgcggt
     6661 tactgcatgg tcatgctcgc gccacgaatc atagtctgtc gccatgcaga tcatctggta
     6721 tggcagttcc agctcgcgcg cgagttttgc ctcgggtaga acgctcatgt tgatgatgcc
     6781 ggcgccccat gatttataga gcttcgattc ggcgcgcgtc gagaactgcg aaccttccat
     6841 gcagatcagg gtttcttttt cgtgcagcgg cagctgcagc ttctgtatcg cctctttcaa
     6901 tatacccgcc aggttagcat ccatcgggtc accgaacgaa acgtgcacca cgataccttc
     6961 atcatagtag gtatcgcgcc ggtggcgggt acggtcgatg atctggtcag gcagcacgaa
     7021 gtgcccggga gcaatttctt ctttcaggga gccgactgcc gagaaggcaa tgactgcttc
     7081 gcaaccgagc atcttgagcg cggccatatt ggcatgcgcc ggtatcgccg tcggtggaat
     7141 aaaatgcccc ttgccgtggc gcggcaggaa tgcaagcgtt acgctcttgc cattttcttc
     7201 ataagttgca atggtaacag catcagaggg tttaccccac ggcgtcttga cgttgatctt
     7261 gtcgataacc ttcatgcctt cgacactata gactcccgta ccgccaataa tcccgatatc
     7321 tactttatgt ttcatactcc acctgccatc tggcgcgcta cttctgcgag caggcgcact
     7381 ccgtacccac tacccgacgc cgtgtaaaca ctgaacggct tcttgatcat tcccacgccg
     7441 gcaatgtcaa gatgcaccca catttgccct tcgtcgacaa aagcgttaag aaattccgca
     7501 gcagtgcagg cgccgccata acgtcccccg atattgttga tgtcggcaat gtctgaattg
     7561 agcagctcac ggtaccgcgg cccaatcggc agcgcccaga ccggctcacg cgactctgcg
     7621 gctgcctttt caac
//
LOCUS       DAOQJF010000143         7633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5243_length_7633_cov_4.729084, whole genome shotgun sequence.
ACCESSION   DAOQJF010000143 DAOQJF010000000
VERSION     DAOQJF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5243_length_7633_cov_4.729084"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLF85_08860"
     CDS             complement(<1..262)
                     /locus_tag="PLF85_08860"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNE19817.1"
                     /translation="MSEFERKVNVELMGRTFTIKGDADPEYMAQVAGYVNAKILEMKK
                     LTASDQTKMLLLTALNLADELFQARRGIQVSPADAQQIEKRTQ"
     gene            complement(264..908)
                     /locus_tag="PLF85_08865"
     CDS             complement(264..908)
                     /locus_tag="PLF85_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19818.1"
                     /translation="MSKSVVACLAASLLFARCFGTIVVPPVTITGQKTATEKQIIGEQ
                     TELEKDVWMISSAKTAEHADLENKPKAVRESIQKENTYTYRAFLKFELFSAHLAQLKK
                     DRVVGENNKGFVTNLLNEKVEIAEEMQKKYSAELAEEPELGRPYRTLTQTVKEINEAR
                     ILAAKGYVESQRRANKDFKATEKEILASQKDKYQQAALKGEMIQAADGSWSAKQ"
     gene            complement(919..1251)
                     /locus_tag="PLF85_08870"
     CDS             complement(919..1251)
                     /locus_tag="PLF85_08870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNE19819.1"
                     /translation="MLRTEILREGNGAVAAKGHNVRVHYTGWLNVGGERGKKFDSSVD
                     RGSPFVFGLGQGQVIRGWDEGVAGMKVGEKRTLFIPADMGYGARGAGGVIPPNADLIF
                     DVELLGID"
     gene            complement(1284..1694)
                     /locus_tag="PLF85_08875"
     CDS             complement(1284..1694)
                     /locus_tag="PLF85_08875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNE19820.1"
                     /translation="MKRTVLIVAVIFTAALAAKGRITKGEGLQYEVIKAGNGKVAQVG
                     QRVQVHYTGWLNEGGKKGKKFDSSVDRRKPFVFGLGQGMVIRGWDEGVAGMKVGEKRT
                     LFIPAALGYGSRGAGAAIPPNADLIFDVELLDCE"
     gene            complement(1750..2472)
                     /locus_tag="PLF85_08880"
     CDS             complement(1750..2472)
                     /locus_tag="PLF85_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE19821.1"
                     /translation="MSRSATSVAVPEAILRVADQTLHSEDRMVIKAYGFIPVTEYFMR
                     DFVKKLMQKYERPELSTPIAMIIKELTVNATKANFKRIVFNENRVNMSDAADYKRGMK
                     IFREAISDRMSVEYGKKAKQARLNIHASFDFDRDRVIIEVRNNLGMTRNEEARVRAKL
                     REAMRCSDVAEFMMENIDETEGAGLGLVLSLMALKSSGIDPHMLTIKTDYETHTLARV
                     EIPLHDNYLPRRHRFQKQRLMA"
     gene            complement(2559..3434)
                     /locus_tag="PLF85_08885"
     CDS             complement(2559..3434)
                     /locus_tag="PLF85_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNE19822.1"
                     /translation="MNKDNLQAIIAITLSFVIGGFLAWTGAQHGLVMNDYPVMYWCVA
                     AAFAIQFVVFVPAFIFRTEKFYDLTGGLTYLSITLFAISLNPHSDLRSKLIATFICIW
                     AIRLASFLFIRVMRAGEDSRFARIKHRFLRFLMAWTLQGLWVTFSLAAGLTAMTSRPV
                     AMDVFAYVGAAIWLVGFFIESLADYQKGRFRANPANKGKFISTGLWSWSRHPNYFGEI
                     VLWLGIAVMAFPVLQGWQYVTLVSPVFVTILLTRISGIPLLEEAADKKWGGHTEYEDY
                     KRRTSKLVLLPPKAG"
     gene            3582..6653
                     /locus_tag="PLF85_08890"
     CDS             3582..6653
                     /locus_tag="PLF85_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNE19823.1"
                     /translation="MPITAPDSVLKLVQKFQDGYDGYVSKSTRFNETEARVQFINPFF
                     EALGWDVTNKKGALPGFQEVWHEDTVRVEGKSKAPDYSFRLGRQRLFFLEAKKPSVII
                     EKGSEADQAAYQTRRYGYSAGLSLSILTDFHEFAVYDTTIRPKPIDKAHVARILYLKY
                     DEYAERFDEIAAIFSKESCEKGLLHAFAKNKTRFKGTSPLDKDFLADMEDWRLTLAKN
                     LAARNEKLTVEELSRAVQLIIDRILFLRVAEDRGLERYGRLGDCAKAKDAYAELKKYF
                     LDADDKYNSGLFHFSAERGRTSEVDNLTTALTLDNKVLGDIINGLYPPASPYEFSAIT
                     SDILGSAYEQFLGSVISLSKGHRASVEQKPEVRHAGGVYYTPKYIVDFIVESTLGEKL
                     AKTTPKQAKVRVLDPACGSGSFLVGAYEYLMRWALDWYVKHDPLKHPQAVYSPNNQDS
                     AVGKVWRLTIAEKKRLLLTHIYGVDIDANAVEVTKLNLMLKALEDENSATVALAFASR
                     KKDNQTQSLGFRERILPDLDQNIHCGNSLVGLDYFDGRLPETVPSDERRKINAFDYEH
                     AFAGSAGFDIVIGNPPYVRQEILGADFKRYAQKTFKTYAGTADLYTYFIEKSLGLLAK
                     DGTYSVIVANKWLRANYGEPLRQFLRTRRIDHIVDFGDLQVFAGATTYPCILKASNRA
                     PDDFFVCNVPSLDFDNLAALVAQKQFKVSLANLAKSGGWSLVNDAEAKLLAKIKAAGV
                     PLGEYVGGKIYYGIKTGLNEAFVIDDATRQRLIKEDKKSAEVIKPFLAGKDIKRYSQP
                     TSRKWLIIFPNGWTRFEGLLSERTGWDHLSARYPAIAGWLKQFEREAKNRYDQGEYWW
                     ELRPCDYYDCFDAPKIIYPDISQIGNFVLDVHGAYLANTAYMIPSNDLYLLGILDSQL
                     FSYCFSRTGNANRGGFFRWFTDVLELCPIYRINENDPAEVKICDRITSLVESMLALNK
                     QLAAARESGAAAQAQTQLTRQIEATDREIDRLVYQLYGLSEEEVKIVEGAD"
     gene            complement(6690..7301)
                     /locus_tag="PLF85_08895"
     CDS             complement(6690..7301)
                     /locus_tag="PLF85_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNE19824.1"
                     /translation="MKAAYLIDGGFFIQKHKQEFRRHPTAANIREFVDKLHAKHASHA
                     EIFRVYYYDCPPADQEIETAVTKTRIHLRNQSVYATKKQFITDIKHLPYFAVREGVLK
                     FDGWEPKSRMHTPTIDDDYKMKFRQKGVDMKIGLDISWLSMGKIVDRIVLVTGDSDFV
                     PAMKFARRQGIQVCLSTLHHGVIEQLKEDADCHYAESLRDIMN"
BASE COUNT         1825 a         1960 c         1958 g         1890 t
ORIGIN      
        1 cctgcgtgcg cttttcgatc tgttgcgcgt cggccggtga aacctgtatg ccgcgccgcg
       61 cctggaatag ttcgtcggcg aggttgagcg ccgtgagcag cagcatcttt gtctggtcgc
      121 tcgcggtgag tttcttcatt tcaagaatct ttgcgtttac ataacccgca acctgtgcca
      181 tatattccgg gtctgcatcg cccttgatgg tgaaggtcct gcccatcagc tcgacattta
      241 ctttgcgttc gaattctgac atactattgc tttgcagacc agctgccatc ggccgcctga
      301 atcatttcac ctttcagtgc tgcttgttgg tatttgtcct tttgtgatgc gagtatttct
      361 ttttcggttg ccttgaagtc tttgttggcg cgcctctggc tttcgacata accttttgct
      421 gcaaggatgc gcgcctcgtt gatttctttt accgtctgcg tcaaggttcg gtaggggcgt
      481 ccgagctcgg gttcttcggc gagttcagct gaatatttct tctgcatctc ttcggcaatt
      541 tctacctttt cattcaaaag gttggtgacg aagcccttgt tgttttcgcc gaccacccgg
      601 tctttcttga gctgcgccag gtgagctgaa aataattcga acttgagaaa tgcccggtat
      661 gtgtaagtat tttctttctg aatgctttca cgcacagctt tgggtttgtt ttccaggtcg
      721 gcgtgttcgg cggttttcgc cgaagaaatc atccagacgt ctttttcgag ctcggtttgt
      781 tcgccgatga tctgcttttc tgtggcggtc ttctgcccgg taattgtgac cggtggcaca
      841 acgatggtac caaaacagcg cgcgaaaagc agtgaagctg cgaggcaggc aacgacgctt
      901 ttcgacacgc gaatcatatc aatcgatgcc gagcagttca acatcaaaaa tcaaatcagc
      961 gttgggaggt ataactccgc ctgcgccccg cgcaccatag cccatgtcgg ctggtataaa
     1021 cagcgtacgc ttttcgccga ctttcatgcc ggcaacacct tcgtcccaac cccgtatgac
     1081 ctggccctgg ccgaggccga aaacaaacgg gctaccccga tcgaccgatg aatcaaattt
     1141 cttgccgcgt tcaccgccga cgttcagcca gccggtgtag tgcacgcgca cattatgccc
     1201 tttcgctgcg acggcgccat tgccttctct gaggatttca gttcgtaaca tattcgtatt
     1261 tcctgtgaga ctaaactttt acactattcg caatcgagta attcgacgtc gaagatcagg
     1321 tctgcatttg gtggaattgc tgcaccagcg ccgcgcgacc cgtagcccag agcagcagga
     1381 atgaacagcg tacgcttttc gcctactttc atgccggcaa caccttcgtc ccagccacgg
     1441 atgaccatgc cctggcccag gccaaagaca aaaggtttac ggcgatcgac agaggagtca
     1501 aacttctttc ctttcttgcc gccttcattg agccagccgg tgtagtgcac ctgcacgcgc
     1561 tggccaacct gagcgacttt accgtttcct gctttgatta cttcgtactg caaaccctca
     1621 cccttggtga tgcggccctt ggctgcaagc gccgctgtga agatcactgc gacaatcaaa
     1681 actgttcgtt tcatgggagc agcgatgcaa cagccaaagc agccgtcgac caaaaaagca
     1741 gggttgcgcc taggccatca aacgctgctt ctggaaccgg tggcgccggg gcaggtagtt
     1801 gtcatgcagt gggatctcca ctcgggccag tgtatgcgtc tcgtagtcgg tcttgatcgt
     1861 cagcatatgc gggtcaatac ccgaagactt gagtgccatc aggctcagaa cgaggcccag
     1921 gcccgcgcct tcggtttcat cgatgttttc catcatgaac tccgccacgt cagagcagcg
     1981 cattgcctca cgcagtttcg cccgcacgcg cgcctcttcg ttgcgggtca tgccgaggtt
     2041 gttgcgtact tcaatgatta cgcggtcgcg gtcaaagtcg aacgaagcgt gtatgttgag
     2101 gcgcgcctgc ttggctttct tgccatattc gaccgacatg cggtcagaaa ttgcttcgcg
     2161 aaatattttc attccgcgtt tgtaatccgc cgcatcgctc atgttgactc tattttcgtt
     2221 aaagacaatt cgcttgaagt ttgccttggt tgcgttcacg gtgagctctt tgatgatcat
     2281 cgcgatgggg gtagagagtt cggggcgttc atacttctgc atcagcttct tcacgaagtc
     2341 gcgcataaag tattcggtaa ccggaataaa gccgtatgcc ttgataacca tgcggtcttc
     2401 ggaatgcagc gtttgatcag caacacgcag aatagcttca ggtacggcga cagaggtggc
     2461 actcctgctc attctgccat tttaacagaa actacgagcg tgtacattag tttactgacg
     2521 cgctcgtcat taatatagga ggtaagtccc atggatattt agccggcttt cggcggtaac
     2581 agaaccagtt ttgaagttct gcgtttgtag tcttcgtact cggtatggcc gccccatttt
     2641 ttgtctgctg cttcttccag cagcggaatt ccgctgatgc gcgtcagcag aattgtcacg
     2701 aatacgggtg aaacgagtgt cacgtattgc caaccctgca gtacaggaaa tgccataacc
     2761 gcaataccca gccagagaac aatttcaccg aagtagttcg ggtggcgtga ccatgaccaa
     2821 aggcctgttg atatgaactt tcccttgtta gcggggtttg cgcgaaagcg gcccttctgg
     2881 tagtctgcca gtgattcgat gaaaaatccc actaaccaga ttgccgcacc gacgtaggca
     2941 aatacatcca tcgcaaccgg gcgtgaggtc atagcagtca ggccggctgc cagcgaaaac
     3001 gtcacccaga gaccctgcag tgtccaggcc atgagaaagc gcaggaagcg gtgcttgatg
     3061 cgggcgaagc ggctgtcttc gcccgcccgc atgacgcgta tgaacaggaa agatgccaac
     3121 ctgattgccc aaatgcagat aaatgtcgca atcagtttag agcgcaggtc gctgtgcggg
     3181 ttgagcgaaa tcgcgaagag cgtgatcgaa aggtacgtga gcccgcccgt caggtcgtag
     3241 aatttttctg ttctgaagat gaatgccggt acaaacacca cgaactggat ggcaaacgcg
     3301 gcagcgacac accagtacat cacgggataa tcgttcatca cgaggccgtg ctgtgccccg
     3361 gtccaggcga gaaagccgcc gatcacgaaa gagagcgtaa tggcgattat ggcctgcagg
     3421 ttgtccttat tcatctgctt ccggagtacg ggctcgccca atagggcgtc aattgtttgc
     3481 gggtcacagt gggggacagg ttaaataccg ggatttccaa ggtgaccatg gatggggaaa
     3541 aatcccgatt gaccgcgcaa aaaccggatg cagacgcgag cgtgcccatc actgcgcccg
     3601 attcggtact taagctcgtc caaaagtttc aagatggata tgacgggtac gtgagtaaat
     3661 ctacccgctt caacgaaacc gaggcccgag tacagtttat taatccgttc tttgaagctt
     3721 tgggttggga tgtgacgaat aagaaaggtg cgctaccggg ctttcaggaa gtctggcacg
     3781 aagacacagt tcgagttgaa ggtaaatcga aggcacctga ttatagcttt cgcctcggcc
     3841 ggcagcgcct cttctttctc gaagctaaaa aaccgtcggt cattatcgaa aaaggaagcg
     3901 aggccgacca ggcagcgtac caaacgcgtc gctacggcta ctctgctggc ctatcgctca
     3961 gcattctgac agattttcat gagtttgctg tctatgacac gacaataagg cccaagccaa
     4021 ttgataaagc tcatgttgcg cgcatactct acctcaagta cgacgaatac gccgaacgct
     4081 ttgatgagat agcagcaatt ttttcaaagg aatcttgcga aaagggtctg ctgcacgcgt
     4141 ttgccaagaa taagacgcgg ttcaaaggca catccccact cgacaaagac tttttggccg
     4201 acatggaaga ctggcgcctt acgctggcta agaaccttgc ggcccgcaac gaaaagttga
     4261 ctgtagaaga gctgagccgc gcggtgcagc ttatcattga ccgcattctt tttctacgtg
     4321 tcgcggaaga ccggggcctg gaacggtacg gtcgtcttgg cgattgtgca aaagctaaag
     4381 atgcgtatgc cgagctaaag aaatactttc tcgacgcgga cgacaaatac aactcaggat
     4441 tgtttcactt ctcggcggag cgtggtcgca ccagcgaggt cgacaacctg accaccgccc
     4501 ttaccctcga taataaagtc ttaggagaca ttatcaacgg gctctaccca ccggcatcgc
     4561 cctatgaatt ttctgcgatc acttcagaca tactgggcag tgcgtatgaa caatttctcg
     4621 gttcggttat ttcactcagc aaaggtcacc gggcgagcgt tgagcagaaa cctgaagtac
     4681 gccatgcagg cggcgtctac tatacgccca aatacattgt cgattttatc gtagaaagca
     4741 ccctcggaga gaaactcgca aaaacgacac ctaagcaggc gaaggttcgc gtgcttgatc
     4801 ctgcctgcgg ttcgggatct tttctcgtcg gagcctacga atatttgatg cgctgggcac
     4861 tcgactggta cgttaagcat gacccgctaa aacacccgca ggcagtctat tcaccgaaca
     4921 accaagacag tgctgtcggc aaggtgtggc ggctcaccat tgccgaaaag aagcgcctac
     4981 tgctgacgca catctatggt gtcgatatcg atgccaatgc cgtcgaagtg acgaaactga
     5041 acctgatgct caaggcactc gaagatgaga attcagccac agtagccttg gcgtttgctt
     5101 cacgcaaaaa agacaaccag acgcagagtc ttggttttcg cgagcggata ctgcccgacc
     5161 ttgaccagaa catccattgt ggcaattcgc ttgtcggatt ggattatttc gacggccgct
     5221 tgcctgaaac cgtacccagc gacgaacggc gtaagatcaa tgcattcgac tacgagcatg
     5281 catttgcagg ttcggccggc ttcgatattg tcattggcaa cccaccttat gtgcggcaag
     5341 agattctcgg ggcggatttc aaacgttacg cgcaaaaaac ctttaagacg tatgccggca
     5401 ccgccgacct ctatacgtat tttatcgaaa aatcgctggg cctgcttgcc aaagacggga
     5461 cttattctgt cattgtggca aacaaatggt tacgcgctaa ctatggggag ccgctgcgcc
     5521 aattcttgcg taccaggcga atcgatcaca tcgttgattt tggcgatctg caggtgtttg
     5581 ccggcgcgac cacttacccc tgcattctca aggcctcgaa cagagcgccc gacgatttct
     5641 ttgtttgtaa cgtgccctcg ctcgattttg ataacctggc agcgttggtc gcacaaaaac
     5701 aattcaaggt ctcattggca aatcttgcca aaagcggcgg ctggtcgcta gtcaacgatg
     5761 ctgaggcaaa gctgctggcc aagatcaaag ctgcaggtgt accactgggt gaatacgttg
     5821 gcggtaaaat ttactatggc atcaagacag ggttgaatga agcctttgtc atcgacgatg
     5881 caacgcggca gcggctcatc aaggaggaca aaaagagtgc agaggtgata aagccgttcc
     5941 tggccggaaa ggatatcaaa cgctattcac aaccaacaag caggaaatgg cttataattt
     6001 ttccgaacgg ttggaccaga tttgagggtt tgctctccga aagaacgggt tgggatcatc
     6061 tctcagccag atatcccgca atcgccggtt ggctgaagca gttcgaaaga gaagccaaaa
     6121 atcgctacga tcagggcgaa tattggtggg agcttaggcc ctgcgactac tatgattgtt
     6181 ttgatgcgcc aaaaattatt taccctgata tttcacagat cggaaatttc gtcttggatg
     6241 ttcatggggc gtatttggca aatacagcat atatgattcc ttctaatgat ttgtatttgc
     6301 tgggcattct agacagccaa ttattttcct attgtttttc tcgtacaggc aatgcgaatc
     6361 ggggcggatt cttccgttgg tttacggatg ttctggagct atgccccata taccgcatta
     6421 atgaaaatga cccggcagag gtaaagattt gtgatcgcat tacgagcctc gtcgagtcga
     6481 tgctggcatt gaataaacaa ctcgctgcag cgcgcgaatc gggtgcagca gcgcaggcgc
     6541 aaacccagct tacccggcag atcgaggcga cagaccgcga aatcgaccgg ctagtttacc
     6601 agctgtatgg gctcagcgaa gaagaagtga agattgttga gggggccgat taggttctcg
     6661 gcggccagaa ctattgctgc atttgttacc taattcatga tatcgcgcag cgattcagca
     6721 tagtggcagt cagcatcttc tttaagttgt tcaatgactc cgtgatgcaa ggttgacaag
     6781 caaacctgta tgccttgtcg gcgtgcaaac ttcatagcag gaacaaagtc cgaatcgccg
     6841 gtcacgagca cgattcgatc gacgatcttg cccatgctca accacgaaat atcgagacca
     6901 attttcatat ccacaccttt ttggcgaaat ttcatcttgt agtcgtcatc gattgtaggg
     6961 gtatgcatcc gggacttcgg ctcccagccg tcaaacttca gaacgccctc cctcaccgcg
     7021 aaatacggca ggtgcttgat atcagttatg aactgcttct tggtggcgta aacagactgg
     7081 tttctcaaat ggatgcgcgt tttcgtaact gcggtctcta tttcctgatc agccggtggg
     7141 cagtcatagt agtaaacccg aaatatctcc gcatgagaag catgctttgc gtggagctta
     7201 tcgacaaatt ctcttatatt ggctgcagta gggtgtcgcc ggaattcttg cttatgcttc
     7261 tgaatgaaaa aaccgccatc gattagataa gccgccttca taaaaacgag ccttgctggg
     7321 acaccgcgag gtgtttacat agaacccagc tcggctctgt atgggtttag aatcggcaaa
     7381 atgagagctg ttgtcaaata atttcccggg tccagtaatt cggtgcgtat ctgccagaca
     7441 tgtctccaga tatggctagc ctgagttagg ccacgaagcc agtccatcca gtcagtatga
     7501 gacataacac ccaaaactcc gattacccac tgaggcttat gtcctgaaaa aaaattttgc
     7561 cacatcagaa aacttagatt atattagcac tgtgattgat agagtgctat cactcacaaa
     7621 tacagagcgc cgc
//
LOCUS       DAOQJF010000144         7584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5295_length_7584_cov_5.384248, whole genome shotgun sequence.
ACCESSION   DAOQJF010000144 DAOQJF010000000
VERSION     DAOQJF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5295_length_7584_cov_5.384248"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..176)
                     /locus_tag="PLF85_08900"
     tRNA            complement(104..176)
                     /locus_tag="PLF85_08900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Thr,seq:tgt)
     gene            complement(229..717)
                     /locus_tag="PLF85_08905"
     CDS             complement(229..717)
                     /locus_tag="PLF85_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNE19825.1"
                     /translation="MSAYSFSPPFEQRFLELEKKFPKRSSLVLWSLHLVQEDIGYITP
                     EAVDYVAARVGVSPAWVKGVVSFYSMFREKPIGKYHLNVCSNLMCQLMGSDKIEQVIQ
                     EKLGIGDGETTPDGKFTYTRQVECLAACGFAPAMLLNNDFHELLTPENVARLIDDLSA
                     KG"
     gene            complement(801..1463)
                     /locus_tag="PLF85_08910"
     CDS             complement(801..1463)
                     /locus_tag="PLF85_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNE19826.1"
                     /translation="MAESSPYVAFFDLDDTLIRKNSGKVFWDYCFQNGIYTLDQLGFL
                     GLSLVRFITGFDETEEFVRNWAKCFRGWPETKMEQITRELFDKEIRTLIREPAKHEIS
                     RHKEQGARTVILSASTRFVCEPVREHLAMDHVLCTGLGVENGRFTGDFSSPYVFGEQK
                     YLSALSYCQEHGFDLSSCYYYGDAYTDRFVMEAVGNPVCVTPDRHLRDYARQKNWKIV
                     EW"
     gene            complement(1560..3878)
                     /locus_tag="PLF85_08915"
     CDS             complement(1560..3878)
                     /locus_tag="PLF85_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNE19827.1"
                     /translation="MQLPARAWDFEGCLFDAYNVGSEVWLWILGEHHRLFLATDTCFP
                     VIYIHASNDILAKVTARLAQIGALASVPRLVNKTLFYENREVEVLRVEIADPAILPRL
                     KRRLFTLYGRFDIYHSDVDPVTGYLAARNLYPLAPVRVRTKIEGRRNRVQQIEATESA
                     WSFEYRLPQLKIMQMSLKFSHRLGVRESNPLLLQVDDRRYAIAEKNPVGYVHRLNEIL
                     RAENPDCIISSYGDHVIFPFLFSAAQAGELTLELDRDLSTQVVRSIKTKGSSFNTYGS
                     WIFRAASYPLYGRWHIDQQNSFTFKHTDLAGTLELSRISGMGVQRLARASTGNALTEM
                     ESDVAMRKNYLVPWQKSALENRKTWYELQLYDKGGLVFQPDTSKGVVRHNVAQLDFSQ
                     MYPTIMNIHNVSPETINCPCCAGLSDVPRVPETGYHICVKRRGVVSDSLQHILERRRY
                     YKQKLTEDLPEPDRQRYQQRVDSLKWMNVVSFGYLGFRNAKFGKLESHESVTAFGRDK
                     LLTAIALAERHGFELVHAITDCIFITRSDDRPIHAESLQRLCDEIYTATSVEMSNEGI
                     YSWALFLPSRTDARMPVANRYLGRFEGGQLKMRGIAVRRKDTPQYIADFQRSLLLLMV
                     DCKDSREVAATWPRALELYKECRRILLGGEVPWVDLLLRRTVSHELDGYHVWNGTSLT
                     MRELAEQGITVSPGEKVRYLVAMRQSTDAQRRFVSEEKAAASGRSVLPYDRRYYEKLL
                     YEAFYEIYGPFVPGMPRADERDQLSLFRCDPL"
     gene            complement(3882..4325)
                     /locus_tag="PLF85_08920"
     CDS             complement(3882..4325)
                     /locus_tag="PLF85_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19828.1"
                     /translation="MENWKNNNIKTTVSVPAEILDTFRKYHSLLPEVLHLVLPTALEQ
                     QPRRDRATEYNHAEAKFEVVQVYWPLEMYNKLHAVAASQRVSVSFLVYRLFLLLLNGI
                     TIIPKNNFSNYVICFQEWSSEAVFFEEGIQYSHKKIELPPEPPAA"
     gene            complement(4306..4611)
                     /locus_tag="PLF85_08925"
     CDS             complement(4306..4611)
                     /locus_tag="PLF85_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19829.1"
                     /translation="MGRTVAPYSIVIEQVLSRFENFRRTLRREDREAFDELMRVAKMQ
                     VQAGVMAQHPNAFDSMSMAMLVHLKREIRELKKQINTENTGSGASGIIGELYGKLEK"
     gene            complement(4613..4816)
                     /locus_tag="PLF85_08930"
     CDS             complement(4613..4816)
                     /locus_tag="PLF85_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19830.1"
                     /translation="MKKQKNSLIRSEKAEDLHQKLLRISLWDSLNKDRKQPISFAFKV
                     NPNDVINRALSVEYLNRMRERHG"
     gene            complement(4875..5606)
                     /locus_tag="PLF85_08935"
     CDS             complement(4875..5606)
                     /locus_tag="PLF85_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19831.1"
                     /translation="MWHQTNNFQTGVNPHQGLSLADAWQLWNYRGNNRSVAYLTGISD
                     IFPLTQIWQTRMAIEGVRTHTIDCAIRFNTYTIAEEALRQSVAPEGIADSVTVTRAFT
                     PYQILDAVKELKKNEVVFVLAPAKQFFDGDVAFDEGLFLIQKLVRFFADAHERGVALF
                     VVEKPSYAAPAFSTFLSDMKQLTQRSRWELSPFGASNGRRLRITEGHAYRDILSLPAE
                     AAKPRMLPVRAPVTMPPAQMQFSFS"
     gene            5773..6375
                     /locus_tag="PLF85_08940"
     CDS             5773..6375
                     /locus_tag="PLF85_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNE19832.1"
                     /translation="MKKLHPTQKQLLDLLSKLSGEGYTIRELQESLSLSTPSLVFFHL
                     QQLEKKGYIERNPMNPADYRVRQPPEESGLFLVNLYGLARCGPSGTILSGDVEERIEL
                     PPQFAGINLRSPFMVRAHGDSMEPRILDGDLVLFEARSEVPDGSVIACSDNGRAYIKR
                     MVRAGKKIMLYSLNTKYEPFAADPESFRIEGLAQAVLGRI"
     gene            6635..>7584
                     /locus_tag="PLF85_08945"
     CDS             6635..>7584
                     /locus_tag="PLF85_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19833.1"
                     /translation="MLAALLSLTSLQAQASTAAPADTIPRKVLVLDFVNQAKDAKSDY
                     LSVSVAEALLDPLKKTGKFYLLPRASTTLGDRGTEANSPDRSLSAVETRDLAPTFDEA
                     TAIARGKSAGADVVVIGNFVSVENKVQIQTKAIDVHTGQIAVAKTTAGKLDASVFDLI
                     QKLADDMSAAMASALPPLPKQVVVTERVGFGLYAKDFRTHLFVGTGLPLGKPNPYLSP
                     GVGGMADLQFEFLHKYFNPYFSFALTNSSGKKSVENMLIFTALGGAAYTFALPWKLPS
                     IKGLTVSPFLAMGASFGTIRAAPDLETQSFSYNVFTLSTGA"
BASE COUNT         1732 a         2115 c         2042 g         1695 t
ORIGIN      
        1 ctccggagag accagattta cagtctggcg ccattaacca ctcggccacc taccctagtg
       61 taagtacaac ccatcgcgat gcggtgggtt gtacttactt ttgagctgcc tgtcagaatc
      121 gaactgacga cctactgctt acaaggcagt tgctctaccg gctgagctaa ggcagcaaaa
      181 ttgcacaggc aattcgcaga aggaaaaaac ggtaaattag tcagtacgtc aaccttttgc
      241 cgaaagatca tctatcagcc gggcgacatt ttcaggggtc agcagttcgt ggaagtcgtt
      301 attgagcagc atcgccgggg caaaaccaca ggcagcgagg cactcgacct gccgggtgta
      361 ggtaaacttg ccgtcgggcg tagtttcacc atcaccaata ccgagtttct cctggatgac
      421 ctgctcgatc ttatctgacc ccatgagctg gcacatcagg ttactgcaga cattcaggtg
      481 gtacttgcca atcggctttt cccggaacat cgagtaaaac gacacaacac cttttaccca
      541 ggccggtgaa acccctaccc tcgctgcgac atagtccacg gcctccggag tgatataacc
      601 aatatcttcc tgaaccaggt gtaacgacca gagtaccagc gagctgcgtt tagggaattt
      661 tttctcgagc tcgaggaatc tctgttcaaa cgggggcgaa aacgaatatg ctgacatctg
      721 accctgctct agtcctctgc tgctgtgacc gtctcaaaag gggggacgcc gtggcgagcg
      781 aaactagtgg ctaagctccg ttaccattcg acaattttcc agttcttttg ccgggcatag
      841 tcacgcaggt ggcggtcggg cgtcacgcag acgggatttc cgactgcctc catgacgaac
      901 cggtcggtgt aagcgtcacc gtagtaataa caggaagaca gatcgaagcc atgctcctgg
      961 cagtaagaga gcgccgacag gtatttctgt tctccgaata cataggggct ggaaaaatcg
     1021 cctgtaaacc ggccgttttc aacacccagt ccggtacaga gcacatgatc catggcaagg
     1081 tgctcgcgta ccggctcaca tacgaaacgg gtcgaagccg aaagaataac cgtccgtgcg
     1141 ccctgctctt tatgccggct gatttcgtgt tttgccggtt cacgtatgag cgtgcggatt
     1201 tccttgtcaa agagctcgcg ggtaatctgc tccatcttgg tttcgggcca gccgcggaag
     1261 cattttgccc agttacggac aaactcttct gtttcatcaa agccggtaat aaagcgcacc
     1321 agggacaggc cgagaaagcc aagctggtct agagtataaa tgccgttttg gaaacagtag
     1381 tcccaaaaga ccttaccgct gttcttacga atgagggtgt catcgagatc aaagaaagcg
     1441 acgtaaggag aagattcagc cattgccgca ccgaagcgcg gcaaacgtgc ggtccggtca
     1501 agcagctttg gctttcgagt atgtgagtag tcggctgcta ttctgcgtca tggtacgact
     1561 taaagcgggt cgcagcgaaa tagcgaaagc tggtcacgct catctgcccg tggcataccg
     1621 ggcacaaacg gcccgtatat ttcgtaaaat gcctcgtaga gtaacttctc gtagtaccgc
     1681 ctgtcataag gcagcaccga cctgccgctg gcggctgcct tctcttcaga aacaaaccgt
     1741 cgctgcgcgt cggtcgattg gcgcatggcg accaggtagc gtactttttc accgggagaa
     1801 accgttatcc cctgctccgc cagctcgcgc atggtcagcg acgtaccgtt ccacacatgg
     1861 taaccatcga gttcgtggct gaccgtgcgc cgcagcagca gatcgaccca aggtacctcg
     1921 ccaccgagca gaatgcgcct gcattcctta tagagctcca gtgcacgggg ccaggtggcg
     1981 gcgacctcgc ggctgtcttt acaatcgacc atgagcaaca gcaggctgcg ctgaaagtca
     2041 gcgatgtatt gcggagtgtc ttttctgcgc acggcaatac cacgcatctt gagctgacct
     2101 ccctcgaaac gaccaaggta cctgttggcg accggcatgc gtgcatcggt acgcgaaggt
     2161 aaaaacagcg cccacgaata tataccttca ttactcatct cgaccgatgt cgccgtatag
     2221 atttcatcgc agagcctctg cagcgattca gcatggattg gccggtcatc gctacgcgtg
     2281 ataaagatgc agtcggtaat cgcatgcacc agctcaaacc catggcgctc cgccagcgca
     2341 atagccgtga gtaacttgtc cctgccaaac gccgtaacgc tctcgtgact ttcaagtttg
     2401 ccaaacttcg cattgcgaaa gcccaggtag ccaaagctca ccacgttcat ccacttgaga
     2461 ctgtcgacgc gctgctggta gcgctgccgg tctggctctg gcaggtcttc ggtcagcttc
     2521 tgtttgtaat aacgccggcg ctcgagaata tgctgcagcg agtcagagac cacgccgcgg
     2581 cgcttgacgc agatgtggta gccggtttca ggcacacgcg gtacatcgct gagccccgca
     2641 cagcacgggc agttgatcgt ctcgggcgaa acgttgtgga tgttcatgat tgtcgggtac
     2701 atctgcgaaa agtcaagctg cgccacgttg tgccgtacca cccccttgct ggtatcgggc
     2761 tgaaagacca aaccaccctt gtcgtagagc tgtagctcgt accaggtctt gcggttttcg
     2821 agcgcactct tctgccacgg caccaggtag ttcttgcgca tcgcgacgtc ggattccatt
     2881 tcggtgagcg cattgcctgt cgaagcgcga gccaggcgct gcacgcccat gccgctgata
     2941 cgtgagagct cgagagttcc cgcgagatcg gtgtgtttaa aagtgaagga gttttgctgg
     3001 tctatgtgcc agcggccgta gagcggatat gaagccgcgc gaaaaatcca gctgccgtag
     3061 gtattgaaac tcgagccttt cgtcttgata ctgcggacta cctgcgtcga gaggtcacga
     3121 tcgagctcaa gggttaattc ccctgcctgc gccgccgaaa acaaaaacgg aaaaatcaca
     3181 tggtcgccat aagaagaaat aatgcagtcg ggattttcag cgcgcaggat ttcgttaagc
     3241 cggtgcacgt agccaacagg atttttttcg gcaatcgcat agcggcggtc gtcgacctgc
     3301 agcagcagcg gattcgattc gcgcacgccc agccggtggc tgaacttcag cgacatctgc
     3361 atgatcttca gctgcggcag acggtactca aaactccagg cgctctcggt ggcttcgatc
     3421 tgctgcacgc gattgcggcg cccctctatt ttcgtgcgta cacgcacggg cgccagcggg
     3481 tacaggttgc gtgcggcaag atagcccgtc accggatcga catcagaatg gtagatatcg
     3541 aaccggccat agagggtaaa cagccggcgt ttcagccggg gcaaaattgc cggatcggca
     3601 atttccacac gcagcacttc aacttcgcgg ttttcataga agagtgtttt gttaaccagg
     3661 cggggtactg aggccagcgc cccgatctgc gccagccggg ctgttacctt cgccagaatg
     3721 tcgttgctgg catgtatgta gataaccggg aaacaggtat cggtggcgag aaagagccga
     3781 tggtgctcgc ccagaatcca gagccagact tcgctgccga cattataggc gtcgaacagg
     3841 cagccttcga agtcccaggc ccttgcgggt agctgcatgg ttcatgcagc gggtggttcg
     3901 ggtgggagtt cgatcttttt gtgggaatat tggatacctt cttcgaaaaa aacagcttca
     3961 gacgaccatt cctggaagca aatgacatag ttactgaaat tgttcttcgg aatgattgta
     4021 atgccattca ataataacag aaaaagccgg tagaccagaa atgacacact cacacgctgc
     4081 gaagccgcca ccgcatgcag tttattatac atttccagtg gccagtaaac ctgcaccact
     4141 tcgaattttg cttcggcatg gttatattcc gtcgcccgat cacgcctggg ctgttgctct
     4201 agcgcagtgg gcaagaccag gtgcaacacc tctggcaaca gcgaatggta ttttctaaaa
     4261 gtatcgagaa tctctgcagg aacagagacg gtagtcttga tattgttatt tttccagttt
     4321 tccataaagt tcccctataa tgcccgaggc acctgacccg gtattttctg tgtttatttg
     4381 ttttttgagt tctctaattt cacgcttgag gtgcaccagc atcgccatgc tcatcgaatc
     4441 gaacgcattg gggtgctgcg ccatgacgcc ggcctgtacc tgcattttgg caacgcgcat
     4501 gagttcatcg aacgcctcgc ggtcttcgcg gcgcagtgtg cgcctgaaat tttcgaagcg
     4561 cgagagtacc tgttcaatga caatcgagta tggtgcaacg gtacgaccca tatcagccgt
     4621 ggcgctcgcg catgcggttc aggtactcga cggatagcgc gcggttgatg acgtcgttgg
     4681 ggttcacctt gaacgcaaag gaaatcggct gtttgcggtc tttattcagg gagtcccaga
     4741 gagagatacg cagcagcttc tggtgcaggt cttcagcctt ttcacttcga ataagagagt
     4801 ttttttgctt tttcatcatc actccttaaa tgtggcccgg gacgcttgcg cgccccgggc
     4861 gagtctcctt gttgtcagct aaaagaaaat tgcatctgtg ccggcggcat cgtgaccgga
     4921 gcgcgtacag gcagcatgcg cggtttggca gcttctgcag gcaggctcag gatatcacgg
     4981 taagcgtgtc cctcggtgat tcgcaggcgg cgcccgttcg aggcaccaaa cgggctgagc
     5041 tcccagcggc tgcgctgggt tagctgcttc atgtcagaaa gaaatgtgct gaacgcgggt
     5101 gcggcgtatg acggcttttc cacgacgaag agagccaccc cgcgttcatg cgcatcggca
     5161 aaaaaccgta ccagcttctg gatcagaaac aatccttcgt cgaaggcgac atcgccgtca
     5221 aaaaactgtt tcgcaggtgc aaggacgaag acgacttcat ttttctttag ctccttaact
     5281 gcatcaagaa tctggtatgg tgtaaatgcg cgggtaacgg taacgctatc ggcgatacct
     5341 tcaggagcca ccgactgccg cagggcctct tcggcgatgg tataggtatt aaagcgtatt
     5401 gcacagtcga tggtatgcgt gcgcacgcct tcgattgcca tacgcgtctg ccagatctgc
     5461 gttaaaggaa agatgtctga aataccggta aggtacgcga cagaacgatt attacctcta
     5521 tagttccaga gctgccacgc atcggcaagc gatagtccct ggtggggatt taccccggtt
     5581 tggaagttgt ttgtctggtg ccacatattt tttcctcccc tcaccccggg aaccaaaccc
     5641 ctcccacggc ccctcccctt atcaagggga ggggaggccg aaggcggggt ggggtcggga
     5701 tgaggttttt actttacaac cactctatag aacaagaata gaaaagcaac tattttgttc
     5761 tatttttgtt ctatgaaaaa actccacccc acccaaaagc aacttctcga cctgctcagc
     5821 aaactttcag gcgaaggtta taccatacgc gaactgcagg aaagcctgag cctttcgacg
     5881 cccagcctgg tcttctttca cctgcagcag ctggaaaaaa aagggtacat tgaacgcaac
     5941 cccatgaacc cggccgacta ccgggtacgc cagccacccg aagaatcagg cctcttcctg
     6001 gtcaacctgt acggcctcgc gcgctgcggc ccctcgggca ccatactcag cggcgacgtc
     6061 gaagaacgca tcgagctgcc gccgcagttt gccggcatca acctgcgctc cccctttatg
     6121 gtgcgcgcgc acggcgactc gatggaaccg cgcatactcg acggcgacct ggtactgttc
     6181 gaagcgcgca gtgaagtacc cgacggcagc gtcatcgcct gctcggataa tggcagggcc
     6241 tatatcaagc gcatggtgcg cgcgggcaaa aaaatcatgc tctattcact caataccaag
     6301 tacgaaccct tcgccgccga ccccgaaagt ttccgcatcg agggcctggc gcaggcggta
     6361 ttggggagga tatgagccat aaccgaatcg cgcacatcat tcccgaagac gcagacgaca
     6421 gcaaagcccc cgtcagctaa ttcagcaagc cacggtttca taagcctgtt gattcttgca
     6481 cattgaaacc tagcccaggg ctgttttgcc cataaatagt ttgacgcata aactctaccg
     6541 ggtacgattg ccgctcaata tgcgggcctc gaaatttact tcattagcca ccgaattaac
     6601 agttcgactc cgctcactgc aagttcacgg gctaatgctg gccgcgctgc tgtcccttac
     6661 ttcactccaa gcacaagcaa gtaccgccgc gcctgctgat acaatccccc gcaaggttct
     6721 cgttctcgat ttcgttaacc aggcgaaaga cgccaagtct gactacctga gcgtcagcgt
     6781 cgctgaggcg ctgctcgacc cgctgaagaa aacggggaag ttttatttgc tgccgcgcgc
     6841 ctcgactacg ctcggcgacc gcggcactga agcgaattcg ccagatcgct cgttgagcgc
     6901 agtcgaaacg cgcgatctgg cccccacttt cgacgaagcc accgcgatcg cccgcggcaa
     6961 aagcgctggc gcggatgtgg tggtcatcgg caactttgtt tctgtcgaaa acaaggtaca
     7021 gattcaaacc aaggcgatcg atgtgcacac ggggcagatc gcggtggcga agacgactgc
     7081 gggcaaactc gatgcgtcgg tgtttgacct aatacagaag ttggccgatg acatgagtgc
     7141 agcgatggcg agcgcgttgc cgccactgcc gaagcaggtg gtagtgactg agcgcgtcgg
     7201 ttttggtctt tacgctaaag attttcgtac tcatctcttt gtgggcactg gtttgccgct
     7261 cggcaaaccg aacccgtacc tgtcacccgg cgtgggcggc atggccgatc tgcagtttga
     7321 atttctgcac aaatacttta atccctattt ctctttcgcg ctgactaatt cgtcaggcaa
     7381 aaaatccgtc gaaaacatgc tgatcttcac cgcgctgggc ggcgcggcat acacgtttgc
     7441 actgccgtgg aaactcccct ccataaaggg gctaaccgtc agcccgtttc tggcaatggg
     7501 cgcgtcgttc ggcaccattc gcgcagcccc cgatctggaa acgcagagct tcagctacaa
     7561 cgtgttcacg ttgtcgacgg gtgc
//
LOCUS       DAOQJF010000145         7542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5346_length_7542_cov_5.202217, whole genome shotgun sequence.
ACCESSION   DAOQJF010000145 DAOQJF010000000
VERSION     DAOQJF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5346_length_7542_cov_5.202217"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLF85_08950"
     CDS             <1..459
                     /locus_tag="PLF85_08950"
                     /inference="COORDINATES: protein motif:HMM:NF019940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HNE19834.1"
                     /translation="LRYLARIQRRINVLHTQAHKKKQIGLRHRIYADLISFPADPSRG
                     EKEKVRGERKLAMQAAQLIWHERRDELLRLVHNDASEEISRIGMHIARMLEIFLRKES
                     AGRELEFFLQELQRETNTIASKAQDSEINSLTVVMKTEIEKLREQVRNLE"
     gene            462..728
                     /locus_tag="PLF85_08955"
     CDS             462..728
                     /locus_tag="PLF85_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNE19835.1"
                     /translation="MKLLHVGFSNSVSIDKIVAIISPETAVAKRLRDMGRENNNLIDC
                     TMGRKLRSMVLTDSDFIFLSAMRPEALTQRMEKPSTPEEADPAD"
     gene            complement(730..3387)
                     /locus_tag="PLF85_08960"
     CDS             complement(730..3387)
                     /locus_tag="PLF85_08960"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNE19836.1"
                     /translation="MEDSQAQQLEKKIAALESEIFSLRSEFERARRDQNKVTANSALP
                     TQPRPVKLTPAEVIAPASTVPVSAIATPATNTTQLAESSFSWEWLIGGNIIGKIGIVT
                     LIVSTALFMVYALDQGWLSEWIRLLMLQGAFAGLSYASYRLYAKQYRYVPEILAITAL
                     AANTIAIYSAHFVYRFLGRGETMAMMFAVMCLALLFARHIRSAALSMILFGGFFALPV
                     IHSRGINEPKAYFLYLFAVNLLYFLLQVRSPQRQSAASVHTIWVIALGNALSVFGWVG
                     TYNQYATSALVFCTLTLAILLHSAHAGLWPEHQRHVLRPAAILFVNLLGAMMVASVLS
                     TNKMFSSEVTALAMLCVAAVNFLVLQVLASDRRHVPSAALVALLLMTVGISIMLDGPA
                     ERLVQAVLLTLAMYAAARSNDRLLFSGTAAANGVNFIALLGSLSYGSENWFLVNFQAA
                     GLLFYTVAAVWLRMRSLWPEKFNFGPVLTSIALLASFVGIVTELQRIITGKDARLLML
                     TLVFASYALVLLVIGFRRQKVWFRQAGLAFMGLAILKFYLVDIWQWDKSVRILAGIIM
                     GGGLVIISFYYEKFRGKFRELGAILLLAGIALPSGDIEATEKFRPNRYRYVKELALPA
                     EGIEGKRYGSTLVDAELYKAAGENDLRLVHDGKIVPYSRQMRKKGSDDKVEKEIPVGE
                     LVYSVDGENTAIYVFENRDRIRISRLRLTFQEQDFTREVSIYQRPGKFQNNTLAKSLT
                     LTRKAGKPEFHTIDFPVVSGELQVQIKNDDDEPIKLSQFIAVSDKEYLLFRLPEKLAD
                     SGKPLKLFYGADYAQKPKYDIAENLNEEDSLAEFVLRPQAANPEFKLTLFDPPYSVWM
                     FRAVFWLLLALIAWRMYSLYRKDKTEAAA"
     gene            complement(3439..3930)
                     /locus_tag="PLF85_08965"
     CDS             complement(3439..3930)
                     /locus_tag="PLF85_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNE19837.1"
                     /translation="MKFLGIDPGYARLGYGIITATGNRAEFVDAGVFETQAKEAEGKR
                     LADMQAFLQSLFAKHTFTHAALEQVFLRRDLTTGIRLSEARGIVVMNLYQAGISYSEI
                     SPSAMKKTITGSGTAQKKTVQLMTAKLLNLPAQMKIDDAADGLGLAFCAWIKWQQTRM
                     SRS"
     gene            complement(3927..4145)
                     /locus_tag="PLF85_08970"
     CDS             complement(3927..4145)
                     /locus_tag="PLF85_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19838.1"
                     /translation="MRNELSLQILQYLFDRAGKRIPITELEVQLGKDVHALRPAIEDL
                     KTEGFVNEEEYRVEILPSGRHFAQSRWV"
     gene            complement(4160..4834)
                     /locus_tag="PLF85_08975"
     CDS             complement(4160..4834)
                     /locus_tag="PLF85_08975"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNE19839.1"
                     /translation="MAKLVLIRHGQSLWNLHNLFTGWVDVPLSAQGVQEAIAAGKRLS
                     HEKFDIIYTSTLIRAITTAMIVMSENKEGKVPVIQKSNEPKPNDWFKIYSPSTEANTI
                     PVYSAWELNERMYGELQGMNKEETAKKFGDEQVKIWRRSYDVPPPSGEALKNTAERTI
                     PWFQKNIEPKLNEGKNILVAAHGNSLRAIIMYLEKLSEAQVLELELKTGEPIVFEAGG
                     GSIKRA"
     gene            complement(4884..5915)
                     /locus_tag="PLF85_08980"
     CDS             complement(4884..5915)
                     /locus_tag="PLF85_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE19840.1"
                     /translation="MKASSVLVHAPNWVGDHAMAFPFYAALRELLPETKIVLIGRSWI
                     ADLAPDKLGSIVAFKGKEPAADDLQRLRAENFDLGFTLSPSFRSALLLRRLGAKIRYG
                     FASDMRSILLTREKNKFRRQPYNRHEHRALAYLRLLNPLLPAGMIAEDLWEKHRNVKL
                     ATLPFETVEKKFGLRQNTQRVVVCPGSTAASKKYPVGHVIRMIETIANRKPAVEFLLL
                     GAKIDQVECDAISAHFAGKKIKLRSLCAQTSLREAHTIIAWSQVAVANDSGLAHITSL
                     TGSALVTFNGMGRREETSPLTHRKTFFDLQLECSPCFAKVCPRKDQPLACLTGIAPDA
                     VAKAVLYSL"
     gene            complement(5912..7261)
                     /locus_tag="PLF85_08985"
     CDS             complement(5912..7261)
                     /locus_tag="PLF85_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19841.1"
                     /translation="MQKKILPLTLFSLAVILAGHPLYSYRVVLDPGHGGRDIVPHTAY
                     GDKYDRRLDMYLDKFREGARHRGVWEFEVMYDIAARAKALLDLTQNEEGRPKFAKILA
                     KYGKAPTNLPAIEAYLSRPAGYIENYFENRDDVNAPFRLYDYPDQKTGEIRPGTISRI
                     NALEPELVITLHLTHTEAPASGAMATVITPGYRTYELAQRYVRSGSAGRQKIRSQFAK
                     TAWNRWFISDDRYKVFPSFMADAFIYYIGFWSKPDGVGTDFTRFRGYRHNLVQWAYAD
                     SDSIVESLYGKQGERYATSLAAFEPLGPFWEREKGEAENWRREGGPEGYGGDNHYAGM
                     EVLRYTRKAFVVNGFDTVATAPKILHPYMSTWAVPTYVNAIAAFVELAHTTSKRDHLR
                     MAKHRQIYAEAIAVSAYSLLYGLEQKSAKSFPKGEPLNLARYQKLAGGNYFRRAKTK"
BASE COUNT         1618 a         2074 c         2173 g         1677 t
ORIGIN      
        1 ctgcgctacc tcgcgcgcat acagcgtcgc atcaacgtcc tgcacacgca ggctcacaag
       61 aaaaaacaga ttggcctgcg ccaccgcatc tatgcagacc tgatcagttt tcccgcagac
      121 ccgtcgcgcg gcgaaaaaga aaaagtccgc ggcgaacgca aactcgcgat gcaggcggcg
      181 cagctgatct ggcacgaacg cagggacgag ctgttgcggc ttgtgcacaa tgacgccagt
      241 gaagaaatca gccgcatcgg catgcacatc gcgcgcatgc tcgaaatttt cctgcgcaaa
      301 gaaagcgcgg gccgcgagct cgagttcttt ttgcaggagc tgcagcgcga gaccaacacg
      361 attgcctcga aggcgcagga cagcgaaatc aacagcctga ccgtggtaat gaagaccgaa
      421 attgaaaagt tgcgcgaaca ggtgcgcaac ctggagtaat tatgaaacta cttcacgttg
      481 gcttttccaa cagtgtttca atcgacaaga tcgtggcgat catttcaccc gaaacagcag
      541 ttgccaagcg gctgcgcgat atggggcgcg agaataataa tcttatcgac tgcaccatgg
      601 ggcgcaagct gcgctctatg gtactgactg atagcgactt tatctttctt tcggccatgc
      661 gccccgaggc tttgacccag cgcatggaaa aaccctcgac tcccgaagaa gccgacccgg
      721 cagactgact taggcggccg cttcggtttt gtccttgcgg tagagcgagt acatccgcca
      781 ggcaatgagc gctagcagca gccagaagac agcccggaac atccacaccg agtagggcgg
      841 atcaaaaagc gtcagtttga attcggggtt cgctgcctgt gggcgcagta cgaactccgc
      901 gagactgtcc tcttcgttca ggttttctgc aatgtcgtat ttcggcttct gcgcatagtc
      961 ggcgccatag aaaagtttca gtggtttccc cgaatctgca agcttctccg gcagcctgaa
     1021 gagcaggtat tccttgtccg agacggcgat aaactggctg agtttgatcg gttcatcgtc
     1081 gtcgttcttg atctgcacct gcaattcgcc cgaaacgaca ggaaaatcta tggtgtggaa
     1141 ttcaggtttg cccgctttgc gcgtgagcgt caggctcttg gcgagcgtat tgttctggaa
     1201 tttgcccggt cgctggtaaa tacttacctc gcgcgtgaaa tcctgttcct ggaaggtgag
     1261 gcgtagccgg ctgatacgga tacggtcgcg gttctcgaag acgtagatcg cggtattctc
     1321 accgtcaacg gagtagacca gttcacccac gggaatttct ttctcgacct tgtcgtcgct
     1381 gcctttcttg cgcatctgcc ggctgtacgg aacgattttt ccgtcgtgca ccagccgcag
     1441 gtcattttcg cccgcagcct tgtacagctc ggcgtcgacc agagtgctgc cgtaacgttt
     1501 gccctcgatg ccttcagctg gcagagcgag ctctttgaca tagcggtagc ggttgggccg
     1561 gaatttctcc gtggcctcga tgtcgcccga aggcagtgcg atgcccgcca acagcaggat
     1621 cgctcccagt tcgcggaact tgccgcggaa tttttcgtaa tagaaactga tgatcacaag
     1681 accacctccc atgatgatgc ccgcaagaat gcggaccgat ttgtcccatt gccagatatc
     1741 cacaagatag aatttcaaaa ttgccagccc catgaaagcc agcccggcct ggcgaaacca
     1801 gactttctgc cggcgaaagc cgatcaccag caagaccagc gcatacgagg cgaagaccag
     1861 cgtcagcatg agcaaccttg catctttgcc ggtaatgatg cgctgcagtt cggtgacgat
     1921 gcccacgaaa gatgccagca gcgcgatcga ggtgagtacc gggccaaaat tgaatttttc
     1981 gggccagagg gagcgcatac gcagccagac tgcagctacc gtatagaaaa gtaatcccgc
     2041 cgcctggaag ttcaccagga accagttctc gctgccgtaa ctcagtgagc cgagcagcgc
     2101 tatgaagttg acaccgttcg cggcagcggt gccggaaaaa agaagtctgt cgttgctacg
     2161 cgcggctgcg tacattgcga gtgtgagcag cactgcctgc accagtcgct ccgctggccc
     2221 atcgagcatg atcgagatgc cgactgtcat caacagcaac gcgacgagag ctgccgaggg
     2281 cacgtgcctg cggtcagacg caagcacctg caggacaaga aaattcaccg cggcaacaca
     2341 gagcattgct aacgcggtca cttcgctgct gaacatttta tttgtcgaaa gtacagacgc
     2401 aaccatcatg gcacccagca ggttgacaaa caatatcgct gcgggccgca gcacgtgccg
     2461 ctggtgctct ggccaaagac cggcatgggc tgagtgcagc agaattgcca gtgtcagagt
     2521 acagaacacc agcgcactgg tcgcgtactg gttataagtg cctacccagc caaagacact
     2581 cagcgcgtta ccgagtgcga ttacccagat tgtgtgtaca ctggccgcag actgccgctg
     2641 tggcgaccga acctgcagca aaaagtagag taggttgacg gcgaacaggt acagaaaata
     2701 ggctttcggt tcattgatac cgcgcgagtg gatcaccggc agtgcgaaaa atcccccgaa
     2761 caggatcatc gatagcgccg ctgagcgaat gtgccgggca aataacagcg cgaggcacat
     2821 cacagcgaac atcatggcca tggtttcgcc gcggccgaga aagcggtaca caaaatgtgc
     2881 agagtatatg gcaatcgtat tggcggcgag cgccgtaatc gcgagtatct cgggtacgta
     2941 acggtattgt ttcgcgtaga ggcggtagct tgcgtagctg aggccggcaa acgcaccctg
     3001 gagcatcaac aggcgtatcc attcactcag ccaaccctgg tcgagcgcat aaaccatgaa
     3061 gagagcggtc gagacaatca gcgtcacgat cccgatcttg ccgatgatgt tgccgccgat
     3121 cagccattcc caggagaatg aactttcagc taactgggtc gtattcgtag ccggcgtcgc
     3181 aatggccgat acgggtacgg tcgatgccgg cgcgatgacc tcagcaggtg tcagcttaac
     3241 cggcctgggc tgtgtaggca gtgcagagtt ggctgttacc ttattctggt cgcggcgtgc
     3301 gcgctcaaac tcagagcgta gagaaaaaat ttcactttcg agtgcagcga tcttcttttc
     3361 caactgctgc gcctgcgagt cttccataag aatcactgat caggccgccc ctgccgggtc
     3421 aatcgtatcg tgccagtatc agcttcgtga catccgtgtc tgctgccact tgatccaagc
     3481 acaaaaggcg aggccgaggc cgtcggcggc gtcgtcgatt ttcatctgcg ctggcaggtt
     3541 gagcagtttg gcggtcatca gctgcacggt tttcttctgc gcggtacccg agccggtaat
     3601 ggtcttcttc atcgcgctgg gtgaaatttc agagtaactg atgcctgcct ggtacaggtt
     3661 catcaccacg atgccccgtg cctcagacag gcgaatgccg gtcgtcaggt cgcgccgcag
     3721 gaaaacctgt tcgagcgcgg cgtgcgtaaa ggtatgtttg gcgaacaggc tctgcaaaaa
     3781 tgcctgcatg tccgccaggc gtttgccttc ggcttctttt gcctgcgtct cgaagacgcc
     3841 cgcgtccacg aactcagccc ggttgcccgt agctgtaatg ataccgtagc cgagccgagc
     3901 gtacccgggg tcaataccca ggaatttcat acccagcggc tctgggcaaa atgccgaccg
     3961 gacggaagga tctctacccg gtactcttct tcgttgacga agccttcagt cttcaggtct
     4021 tcaatcgccg ggcgcagcgc gtgtacatct ttgcccagct ggacttcgag ttcggtaatc
     4081 ggtatgcgtt ttcctgcgcg gtcgaacagg tactgcagaa tctgcagcga gagttcattc
     4141 cgcacgtctg gctgctacct caggcgcgct tgatactacc accgcctgcc tcgaagacaa
     4201 tcggctcgcc cgttttgagt tcgagctcga gtacctgtgc ttcgctgagc ttctcgaggt
     4261 acatgatgat cgcacgcagg ctgttgccgt gtgccgcaac gagaatgttc ttaccctcgt
     4321 tgagtttggg ctcaatattc ttttggaacc acggaatagt acgttcggcg gtattcttga
     4381 gcgcttcgcc cgatggtggc ggcacgtcgt agctacggcg ccagatcttg acctgttcgt
     4441 cgccgaattt cttcgcagtt tcttctttat tcatgccctg cagctcgccg tacatgcgct
     4501 cgttgagttc ccaggcggaa taaacaggga tcgtattggc ttcggtcgaa ggagaataaa
     4561 tcttaaacca gtcgttaggt tttggttcgt tcgatttctg gattacgggc actttgccct
     4621 ctttgttctc tgacatgacg atcatcgcgg tggtaatagc gcgtatcagt gtagaggtgt
     4681 agatgatatc aaacttttca tgcgacaggc gcttgcctgc ggcgatggcc tcctgcacgc
     4741 cctgtgcaga cagagggacg tcaacccagc ctgtgaaaag attgtgcagg ttccacagcg
     4801 attggccgtg gcgaatcaaa accagttttg ccatgcgctc aactcacaat gagagcgcac
     4861 gcgtaaagca gttggcagta tcttcagagc gagtagagta ctgctttagc taccgcatcg
     4921 ggtgcgatgc cggtcaggca ggccagtggc tggtccttgc gcgggcaaac cttcgcaaag
     4981 cagggtgaac attcgagctg caggtcgaag aatgtcttgc ggtgtgtcag cggcgaggtc
     5041 tcttcacgcc gtcccatgcc gttgaaagtc accagcgcgc tgccggtcag cgaggtgata
     5101 tgcgccagcc ccgaatcgtt ggcgactgct acttgtgacc aggcaataat ggtgtgagcc
     5161 tcgcgtagcg atgtctgtgc gcagagcgag cggagtttga ttttttttcc ggcaaaatga
     5221 gcggagatgg cgtcacactc cacctggtcg attttggcgc cgagcagaag aaactccacc
     5281 gcaggcttcc tgttggcgat ggtctcgatc atccgtatca catgaccgac cgggtatttt
     5341 tttgaagcgg cggtgctgcc ggggcagacg acgacgcgct gcgtgttctg gcgcaggcca
     5401 aactttttct caactgtttc aaaaggcagg gtggccagct ttacgttgcg gtgtttctcc
     5461 caaagatctt cggcaatcat tcccgccggc agcaggggat tcagcaggcg caggtaggcc
     5521 agggcgcggt gttcatgccg gttgtacggt tgccggcgaa acttattttt ttcgcgggtg
     5581 agcagtatgc tgcgcatatc agaagcaaac ccgtaacgta ttttcgcccc cagccggcgc
     5641 agcagcaagg cggagcgaaa actgggcgaa agggtaaaac cgagatcaaa attttccgcg
     5701 cgcagccgct gcaggtcgtc agccgcgggc tctttgccct tgaacgcgac tatgctgccg
     5761 agtttgtcgg gggcaagatc ggcgatccag gagcggccaa tgaggacaat ttttgtttcg
     5821 ggtaacagct cgcgcagcgc ggcataaaaa ggaaaagcca tcgcatggtc gccaacccag
     5881 tttggcgcgt ggacaagtac gctgctggct ttcattttgt tttcgcgcgc ctgaagtagt
     5941 tgccgcctgc tagtttctgg tagcgcgcga ggttgagcgg ttcacccttc gggaaactct
     6001 ttgcgctttt ttgttcaaga ccgtagagta gcgaatatgc tgaaacagcg atagcttcgg
     6061 catagatttg gcggtgtttt gccatgcgca ggtgatcgcg cttagaagtc gtgtgggcca
     6121 gctcgacaaa agcggcgatg gcgtttacat acgtcggtac agcccaggtt gacatgtaag
     6181 gatgcagaat ttttggtgca gtggctaccg tatcaaaacc attaacgaca aaggccttac
     6241 gcgtgtagcg gagtacttcc atacccgcgt agtggttgtc gcccccataa ccttcgggcc
     6301 cgccttcgcg gcgccagttc tcggcttcgc ctttttctct ttcccagaaa ggcccaagag
     6361 gttcaaatgc tgcgaggctt gtcgcatacc gttcgccttg cttgccatag agcgattcaa
     6421 cgattgaatc ggagtctgcg tatgcccact gcacgaggtt atgccggtaa ccgcggaagc
     6481 gcgtgaagtc tgtacccaca ccatccggct tagaccagaa accgatatag taaatgaaag
     6541 cgtcggccat gaagctgggg aaaaccttgt agcggtcatc agaaataaac cagcggttcc
     6601 acgctgtctt cgcaaactgt gagcgaatct tctgccggcc ggcgctgccc gagcgcacgt
     6661 agcgctgtgc cagctcgtac gtgcgatagc caggagtaat gactgtcgcc atggcgcctg
     6721 aagccggtgc ttctgtatga gtcaggtgca gggtgatcac gagctctggc tcgagcgcgt
     6781 tgatgcgcga aatcgtgccc ggccgtattt caccggtctt ctggtcgggg taatcgtaca
     6841 ggcgaaaggg tgcattgaca tcgtcgcggt tctcaaaata gttctcgatg tagcctgcgg
     6901 ggcgcgacag atatgcttct atcgcaggaa ggtttgtggg agctttaccg tatttcgcca
     6961 gaatcttcgc gaattttggt ctgccttctt cgttctgtgt caggtcgagc agggctttcg
     7021 ccctcgcggc aatgtcgtac atcacctcga actcccacac gccgcggtgc cgtgcgcctt
     7081 cgcggaattt gtcgaggtac atatcgagcc gtcggtcgta tttatcgccg tacgctgtat
     7141 gcggcacgat atcgcgcccg ccatgaccag ggtcgagcac cacccggtaa ctataaagag
     7201 ggtggccggc cagaattacg gcgagactga acagggtaag aggcaggatt ttcttttgca
     7261 taaggcattc aggcgtgctt tgattatagc accctgcacg gcgaagcgcg gctttgagac
     7321 agtgcggcaa acatgtttgc cgtgaatcgg ctttacggcg aagaccgccg gcccgagcta
     7381 gcgcctatga actcgcgtgt ccgctgggtg ttgcgcgctg ttgccgcggt gtcgctgctt
     7441 gctgccggct cgctctggtg gtatagccgc acgaccctcg gtgacgcaca ggccctggcc
     7501 cgcggcgaag aggtctatgc ttcgcacgac tgcaccgact gt
//
LOCUS       DAOQJF010000146         7542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5347_length_7542_cov_5.152264, whole genome shotgun sequence.
ACCESSION   DAOQJF010000146 DAOQJF010000000
VERSION     DAOQJF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5347_length_7542_cov_5.152264"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLF85_08990"
     CDS             complement(<1..1184)
                     /locus_tag="PLF85_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HNE19842.1"
                     /translation="MNSIEAYLSMAVLALLILTSAFYTFYLRFLPLRYVIYGFRIALA
                     GLGKEDEGSTLSRSHRAGEINNLRAMFLAGSVQANAAVLLGIPVIVLWGGAASLIWVA
                     LFGVLLATLRFANGTLAVKYREKHENTWLGGAGEIIATTFWGKSGAIVYALIALATAL
                     VWGAAAQAELWHNFYTPPASVGIPATKPILPFQLGEHIFSSQWAPLLIALTGFLLSLG
                     SLRRIGRVAAFITPIVTTLLLILAIFIIAQNPHKAAELAANACSQALSLKSLSAGIIA
                     TLLIFKCAAFTGIVAPEESTGVSAAVTAASINDYAVKQGLVSMSTALFYTTIFIPLLG
                     LAYAVGLPEIKGATVAPGGVKALLAPAPAAAAEMISQVQFSLAHIFSATLGRGGFLII
                     ALS"
     gene            1346..2230
                     /locus_tag="PLF85_08995"
     CDS             1346..2230
                     /locus_tag="PLF85_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNE19843.1"
                     /translation="MSKEFYTEADLANFRDCQSMAYDAVQAVARRLTAGMTEKQAAEM
                     IDEEIKGRGMTRYFHAPFAWFGARTAFQGFERPFDLSRFPASLLPPHFGQKFMPTETR
                     LEEGMGVILDVAPIYNDCAADIGYAFAFGRNEKIEKALDDLAVFRPLILAGVCKEKTM
                     AEIYDDVSEALLDMGYENCHAKYPQGVLGHKVGKIPTRLIPRIPVMRFEVSTFLYLAA
                     EEISHLINPWDNHTALWSDYTRVSADPGLWAVEPHIGTKNFGVKWEEILHITENNAYY
                     LDDNLPHVRRWTDRQVAV"
     gene            2240..3070
                     /locus_tag="PLF85_09000"
     CDS             2240..3070
                     /locus_tag="PLF85_09000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE19844.1"
                     /translation="MAIERKTPENIKPHRIQVSFDESLPQHWFGGNAFLTHMLNTWTA
                     IFPDAERYFVRWSKKALASVTDPKIKKDIQGFIGQETMHAMQHEKLWELLRSQGYNLD
                     PVIFFVEKVAFDWLEKALPDKVNYALVAGLEHFTAMFGEIYFENPELVEQMHPEMRRL
                     FEWHAAEEVEHKAVAYDQLLAIDDSYALRTGVMNVAVVGFYMVTLLNTLYFMAQDKSL
                     YNPKEWLTMYDVFFGKYDLARKTFVKFLEYYKPDFHPWDNQNYHLAQPTLASYEKQAS
                     "
     gene            complement(3106..3837)
                     /locus_tag="PLF85_09005"
     CDS             complement(3106..3837)
                     /locus_tag="PLF85_09005"
                     /inference="COORDINATES: protein motif:HMM:NF017945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNE19845.1"
                     /translation="MDISIFSDHGALLFRYFHIVGGVAWIGVLWYINFIQGGYVGGLD
                     AAGQTIAKQKLFPKVMWYFRYGALLTFITGLGLIALKGFGPGKVGGQIGSDVWNAWFA
                     KIYPGFMFATIMFLNVWFVIWPRQKKVIAAANGEKIDGVPAMARRGFIASRTNTLLSI
                     PMLFFMAAANNLIISGISADKLNLWIIITSVVFILIEANALFASNDPAAKPATVKPIE
                     TVKGVIHMGFVLTILLYVGVELILK"
     gene            3964..4590
                     /gene="msrA"
                     /locus_tag="PLF85_09010"
     CDS             3964..4590
                     /gene="msrA"
                     /locus_tag="PLF85_09010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNE19846.1"
                     /translation="MNSRCIYLLSAALLACSQPVVADKAFSLPRPYVNKDQRLSVAVF
                     AGGCFWCMEPPFEKLAGVKAVISGYAGGTEKNPTYDEVSYGRSSHIESVLVLYDPKQV
                     TYEKLLGVFWRNINPEQEDGQFYDVGSQYKTYVFYGNAAERLAAEKSKAELLRSGKFR
                     RIATQLRPATEFWPAEDYHQDYYKKNPAHYQRYRVGSGRDAYLKAKWN"
     gene            complement(4614..7172)
                     /gene="secA"
                     /locus_tag="PLF85_09015"
     CDS             complement(4614..7172)
                     /gene="secA"
                     /locus_tag="PLF85_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNE19847.1"
                     /translation="MVQFLLKAIFGSKYERDLKRIRPYVDRINALEPEIQKLTDAELA
                     AKTPAFKERIANGEKLISILPEAFAVLRETSLRVMKMRHFDVQLMGAMALNEGKIAEM
                     KTGEGKTLTATLAVYLNALTGKGVHVVTVNDYLASRDAEWMRPLYDFHGLTTGVIKSN
                     MNHAKRREAYAADITYGTNNEFGFDYLRDNMVDHRNYRVQRSHYFAIVDEVDSILIDE
                     ARTPLIISGPADQDAGEYVRVNRVIPSLEDKVDYDVDEKARTVLLTEIGVHKVEKLLN
                     VDNLYAPQNVELVHHVHQCLKAHKLFHRDVDYVVQAGEIIIVDEFTGRLMEGRRYSDG
                     LHQAIEAKEKVEVKQENQTLATITFQNFFRMYEKLGGMTGTADTEAEEFRKIYKLDVM
                     VIPPNRKMIRKDFADKVYRTQREKFNAIADEVKELHLKGQPVLLGTISIENSEKLSKL
                     LHQKGIPHNVLNAKHHDREAEIIKEAGHAGRVTIATNMAGRGTDIILGSGVKELGGLH
                     IVGSERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDELMRIFGSDRLGPIMQRFGMQ
                     EGEALEHRMVTNAIERAQKRVESHHFEMRKHLLEYDDVMNKQRKYIYMIRNEILDNAD
                     VKALIREFTEDAIQIRIGQMLPGKKVSEFNFEALQDYLANVFQIRYTDLEEELRAKSK
                     DEFIAEIREKALQALDAKEQEIGSENMRILEKMIALQVIDQKWKDHLYLMDQLRDGIW
                     TLGYAERNPLVEYRFRAFAMFEDMVAAVKDEVTEFILRAQVKEQLHEEDADEDEYEEV
                     GSAQHSSMAGFDMTDGVAAQAIAQHVNRGPQQSSSEAAKQTGGGSSRRKSSRRR"
     gene            complement(7250..>7542)
                     /locus_tag="PLF85_09020"
     CDS             complement(7250..>7542)
                     /locus_tag="PLF85_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19848.1"
                     /translation="GMYVFSKVWDLNPAAGFGLTYYQLSGGSYSFQPSIAPVAVGSLY
                     YDLLPNLQLGVIGQFILATTGKITTTGADYTLKSATWLTTASLAVSVRYAWF"
BASE COUNT         1700 a         2009 c         2098 g         1735 t
ORIGIN      
        1 gacagcgcaa tgatgagaaa accgccgcgc cccagtgtgg cagaaaaaat atgcgccagc
       61 gaaaactgca cctggctgat catttccgct gcagcggcag gcgctggcgc gagcagcgct
      121 ttcactccac cgggtgctac ggttgcgccc ttgatttccg gcaggccaac cgcgtacgcg
      181 aggccgagca gcggaataaa gatcgtggtg tagaagaggg cggtcgacat cgaaaccagg
      241 ccttgcttca ccgcatagtc attgatcgaa gccgcagtga ccgcggccga tacgccggtt
      301 gattcttcag gcgccacaat gccggtgaac gcggcgcact taaaaatcag cagcgtggca
      361 atgatgcctg cggaaaggct tttgaggctc agtgcctgcg aacacgcgtt cgcagcaagc
      421 tcagctgcct tgtgtggatt ttgggctata atgaagatcg ccaggataag caataatgtc
      481 gtcacaatcg gcgtgatgaa ggcggcaaca cggccgatgc gccgcaggct gccgaggctc
      541 agcagaaaac cggtgagcgc aatcagcagc ggcgcccact ggctgctgaa gatgtgttct
      601 cccagctgaa aaggcagaat cggttttgtc gcgggtatgc caactgaagc cggtggtgtg
      661 tagaagttat gccagagttc tgcctgtgct gcagcccccc agaccagggc agttgccagg
      721 gcgatgagtg cgtagacgat ggcaccactt ttgccccaga aggttgtcgc aataatctca
      781 cccgcgccgc ccagccaggt attctcgtgt ttctcgcggt atttgacagc gagcgtgcca
      841 ttggcaaaac gcagcgtcgc gagcagaacc ccgaagagcg ctacccatat gagagaagca
      901 gctccccccc aaaggacgat gacaggaatg ccgagcagca cagctgcatt ggcctgcaca
      961 ctgccggcca gaaacatcgc gcgcaggtta ttgatttcac ccgcacggtg cgaacgcgat
     1021 agcgtcgaac cttcgtcttc tttaccgagc cctgccagtg caatgcgaaa accatagatg
     1081 acatagcgca gcggcaggaa gcgcaggtaa aaggtgtaga aggcggaggt caggatcagc
     1141 agtgccagta ccgccatgga caggtaggct tcgatgctat tcattgtgcg cagaataata
     1201 aaaagaagga gcccgacaag caatatcgcc ttctgaggcg ggggatatat agctcatccc
     1261 ggggttcagg ttacccgagg ctttgtttca tattgcactg cataaaatga agttgacgct
     1321 ttgttcactg atgcaaggag gcggtatgtc caaagagttt tatacggaag ccgacctcgc
     1381 gaacttccgt gattgccagt caatggccta tgacgccgtg caggcagttg cgcgccggct
     1441 caccgccggc atgaccgaga agcaggcggc cgagatgatc gacgaagaga tcaaggggcg
     1501 cggcatgacc cgctatttcc atgccccgtt tgcctggttt ggagcgcgca cggccttcca
     1561 gggctttgaa cggcctttcg acctcagccg ttttccggcc agcctgctgc cgccgcattt
     1621 tgggcagaaa ttcatgccca ccgaaacccg gctcgaagag ggtatgggag ttattctcga
     1681 tgttgctcct atctataacg actgcgcggc cgacatcggt tacgcctttg cctttggccg
     1741 taacgaaaag atcgaaaagg cgctcgatga tctcgcggtg ttccggccgc tgattctcgc
     1801 cggcgtctgc aaagagaaga cgatggccga aatttatgac gatgtcagcg aggcgctgct
     1861 cgatatgggc tacgagaact gccatgccaa gtacccgcag ggggtactgg gccacaaggt
     1921 cggcaagata ccgacgcgcc tgattccacg cattccggtc atgcgcttcg aggtttcgac
     1981 atttctctac ctcgcagctg aagagattag ccacctgatt aacccatggg ataaccacac
     2041 ggcgctttgg tccgattaca cgcgcgtctc agctgacccg ggcctgtggg cggttgaacc
     2101 acatatcgga acaaaaaatt ttggcgtcaa gtgggaagaa attctccaca tcaccgaaaa
     2161 caacgcgtac tacctggatg ataacctgcc gcacgtgcgc cgctggaccg accgccaggt
     2221 cgctgtataa ggagttaata tggccatcga acgcaaaacc cccgagaata tcaaacccca
     2281 ccgcatccag gtcagcttcg acgagagcct gccgcagcac tggtttggcg gcaatgcttt
     2341 cctgacgcac atgctcaata cctggacggc gatctttccc gatgccgaac gttactttgt
     2401 ccgctggtcg aagaaagcgc tcgcgagtgt gactgacccg aagattaaaa aagacatcca
     2461 gggttttatc ggccaggaaa ctatgcacgc gatgcagcac gaaaagttgt gggagctttt
     2521 gcggtcacag ggctataacc tggatccggt catttttttc gtcgagaaag tggcttttga
     2581 ctggctggaa aaggcgctgc ctgacaaggt taactacgca ctcgtcgcgg gcctcgagca
     2641 tttcaccgcg atgttcggcg aaatctactt tgaaaatcct gaacttgtgg aacagatgca
     2701 cccagagatg cgccggcttt tcgaatggca tgcagccgaa gaagttgagc acaaggcagt
     2761 ggcctatgac cagctgctgg cgatcgacga cagctacgca ctgcgcaccg gtgtcatgaa
     2821 tgtagcagtg gtgggtttct acatggtaac gctgctcaac acgctctatt tcatggcgca
     2881 ggataaatcg ctctataacc caaaagaatg gctcaccatg tatgatgtat tctttggcaa
     2941 gtacgacctt gcgcgcaaga cgttcgtgaa atttttggaa tactacaagc cggattttca
     3001 cccgtgggat aaccagaact accacctggc gcagccgacg ctggcatcgt acgagaaaca
     3061 agcatcctga gcgctacgcg ctcaggatgc ttgtttgaat ttagcctatt tcagaatcag
     3121 ttctacaccc acgtagagca ggattgtcag aacaaagccc atgtgaatga caccttttac
     3181 ggtttcaatc ggctttacag tagctggttt tgctgccggg tcgtttgaag caaaaagcgc
     3241 attcgcttcg atcaggatga aaaccacaga cgtgatgatt atccagagat tgagcttgtc
     3301 tgcagatatt ccggagataa tcaaattgtt tgcagctgcc ataaagaaca gcatcgggat
     3361 agagagcagg gtgttggtgc gtgaagcgat aaaaccgcgg cgagccattg caggtactcc
     3421 gtcaattttt tctccgttag ctgccgcgat aacctttttc tggcgtggcc agatcacaaa
     3481 ccagacgttc aggaacatga ttgtcgcgaa catgaatcca ggatagattt tcgcaaacca
     3541 ggcattccat acatcgctac caatctgacc gccgactttg ccgggaccaa atcctttgag
     3601 tgcgatcaga ccaagaccag tgatgaaagt cagcagtgcg ccgtagcgga agtaccacat
     3661 gactttgggg aagagttttt gcttcgcgat ggtctggcct gcagcgtcga ggccgcctac
     3721 ataaccgccc tggatgaaat tgatgtacca gagtacgccg atccaggcta cgccgccaac
     3781 gatgtgaaaa tagcgaaaca gcaacgcgcc atggtcagaa aatattgata tgtccattct
     3841 ttctcctttg aaatttgagc ttgatttcgg gcttaacccg cgtgataaca atataatgcc
     3901 gcaggtcagg ctttgtcaat aaattgtctg cagggcaaac caaggctccc ggaggatcta
     3961 aatatgaata gcagatgtat ttatctattg agcgcagcac ttttagcctg ttcacagcct
     4021 gtagtcgccg ataaggcttt ctccctgccg cggccgtacg tgaacaaaga ccagcgcctt
     4081 tctgttgccg tatttgccgg tgggtgcttc tggtgcatgg aacccccgtt tgaaaaactc
     4141 gcaggtgtca aagctgtcat ttcaggttat gccggcggta cagaaaaaaa tcccacctat
     4201 gacgaggtgt catacggccg tagctcgcac attgaatcag tgctcgtgct ctatgacccg
     4261 aaacaggtaa cctacgagaa gctgctcggg gtgttctggc gcaacattaa ccccgaacag
     4321 gaagacgggc aattctacga cgttgggtcg cagtacaaga catacgtttt ttacggcaat
     4381 gctgcagaac gattggcagc agaaaaaagc aaggcggagc tactgcgttc aggcaagttc
     4441 aggcgcatag ccacacagct tcgccccgct acagaattct ggccagcaga ggactaccac
     4501 caggattatt acaagaaaaa cccggcgcac taccagcgct accgcgttgg ctcgggtaga
     4561 gatgcgtatc taaaggctaa atggaattga ttcatcaatt ccatttagca gactcagcgg
     4621 cggcgcgagc ttttgcgcct tgaagaaccg ccgccggtct gttttgctgc ttctgaactg
     4681 gactgttgcg gcccgcggtt tacgtgctgc gcaattgcct gcgcggcaac accgtcggtc
     4741 atgtcaaaac ctgccattga gctgtgctgc gcgctgccaa cttcttcgta ctcgtcttcg
     4801 tcggcatctt cttcatgcag ctgctctttg acctgtgcac gcagaataaa ctcagtgacc
     4861 tcgtctttga ccgcggcaac catgtcttca aacatggcaa acgcccgaaa gcggtattcg
     4921 accagcgggt tacgttctgc ataaccgagc gtccagatac cgtcacgcag ctggtccatc
     4981 aggtagaggt gatctttcca cttctggtcg attacctgca gcgcgatcat cttctcgaga
     5041 atgcgcatgt tttctgatcc gatttcctgc tctttggcgt cgagtgcctg cagcgccttt
     5101 tcgcggattt ccgcgataaa ctcgtccttg ctcttcgcgc gcagctcttc ttcgagatct
     5161 gtgtagcgaa tctggaaaac gttggccagg taatcctgca gcgcttcgaa attgaactca
     5221 gacaccttct tgccgggcag catttgcccg atacgaatct gtatggcgtc ttcggtgaat
     5281 tcgcggatca gcgccttgac atccgcgtta tctagaattt cattgcggat catgtagatg
     5341 tacttacgct gcttgttcat cacgtcgtcg tactcgagca ggtgcttgcg catctcgaag
     5401 tggtgcgatt cgacgcgttt ttgcgcgcgt tcaatggcgt tggtgaccat gcggtgctcg
     5461 agcgcttcgc cttcctgcat gccgaaacgc tgcatgatcg ggccgaggcg gtcagacccg
     5521 aagatgcgca tcagttcatc ttcgagagag aggtagaagc gtgagctacc cgggtcaccc
     5581 tggcggccgg cacggccgcg caactggttg tcgatacggc gtgattcgtg ccgctcagag
     5641 ccgacgatgt gcaggccgcc gagctctttg acgcccgacc caagaatgat gtcggtaccg
     5701 cggcccgcca tgttggtggc aatggttacc cggccggcat gacctgcttc tttaataatt
     5761 tctgcttcgc ggtcgtggtg cttcgcgttg agcacgttgt gcggaatacc cttttggtgc
     5821 agtaacttgg agagcttttc tgagttctca atggatatcg taccgagcag caccggctgg
     5881 cccttgaggt gcagctcttt tacctcgtcg gcaatcgcat tgaacttctc gcgctgcgtg
     5941 cggtagactt tatcagcaaa gtctttgcgg atcattttcc ggttgggcgg aatgaccatg
     6001 acgtcgagct tatagatctt gcggaattct tctgcttcgg tatcagccgt accggtcatg
     6061 ccgccgagtt tctcgtacat gcggaagaag ttctggaacg tgatggtggc gagcgtctgg
     6121 ttctcttgtt tgacctcaac cttttctttg gcttcgattg cctggtgcag cccgtcgctg
     6181 taccggcgac cttccatcag gcggcctgta aactcgtcga caatgatgat ttcaccggct
     6241 tgtacgacat agtcgacatc gcggtggaag agtttatgtg ccttgaggca ctggtgcacg
     6301 tggtgtacga gctcgacatt ctgcggggcg tagaggttgt cgacgttgag cagcttttct
     6361 accttgtgta ccccgatctc ggtgagcagc acggtgcgcg ctttttcatc gacatcgtag
     6421 tcgaccttgt cttcgagcga aggtatgacg cggttcacac gcacgtattc gcccgcatcc
     6481 tggtcggcag ggccgctgat aataagagga gttcttgctt cgtcgatcag gatcgagtct
     6541 acctcgtcga caatcgcgaa gtagtgcgaa cgctgcacgc ggtagttgcg gtggtctacc
     6601 atgttgtcgc gcaggtagtc aaaaccgaac tcgttgttgg ttccgtaggt gatgtccgca
     6661 gcgtaggcct cgcggcgctt cgcatggttc atgttgctct tgatgacgcc cgttgtcagg
     6721 ccatgaaagt catagagcgg acgcatccac tcggcgtctc ggcttgccag gtaatcgttg
     6781 accgtaacga cgtgtactcc cttgccggtg agcgcgttga ggtaaacggc aagcgtcgca
     6841 gtgagggttt taccttcacc ggttttcatc tcggcaatct taccttcgtt gagcgccatg
     6901 gcgcccatca gctgtacgtc gaaatggcgc attttcatga cgcgcagcga ggtttcgcgc
     6961 agcacggcaa acgcctcggg cagtatagag atcagctttt cgccgttggc gatgcgctct
     7021 ttgaatgcag gcgttttagc cgccagttca gcgtctgtga gtttctggat ttcaggttca
     7081 agcgcgttga tacgatcgac atacggcctt atgcgcttca ggtcgcgctc atatttggag
     7141 ccgaaaattg ctttgagaag aaactggacc atcgggtcat tttgacctca aagacctgcc
     7201 gtcgataaaa acagcttttg caggcagtgc cgcgcggccg cgcagccaat cagaaccagg
     7261 cgtagcgcac tgaaacggcg agcgaagccg tggtgagcca ggttgccgac ttcaaagtat
     7321 aatccgcgcc ggtggtcgtg atcttgccgg tggttgcgag tatgaactgc ccgataacgc
     7381 cgagctgcag gttgggcagc aggtcgtagt agagtgagcc tactgcaaca ggggcaatac
     7441 tcggctgaaa gctatagctc ccaccgctga gctggtagta agtaaggcca aaacctgcag
     7501 cagggttgag atcccagact ttggagaaaa cgtacatgcc gg
//
LOCUS       DAOQJF010000147         7534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5359_length_7534_cov_5.686857, whole genome shotgun sequence.
ACCESSION   DAOQJF010000147 DAOQJF010000000
VERSION     DAOQJF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_7534_cov_5.686857"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4204)
                     /locus_tag="PLF85_09025"
     CDS             complement(<1..4204)
                     /locus_tag="PLF85_09025"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNE19849.1"
                     /translation="MRTTSPAMTTFVRGGMLALSLFAANCSGRTYNTFFQPFDLVFGS
                     QGNSSVNVSLLTATPLTNTRVMLTFNKPVSLASGQLVSNYRITDSNGNLLNILAASRD
                     PNNSRVVFVDTIPQTGGTTYTVTASGIVGVDGSSLGSSNSATFTAPTNADQTGPSFGS
                     VSALSSTSVEVYFTEAVEKASSETATNFDIYTNSGCSAGNVNVTGAVRDSVNFAKVTL
                     TSAVMTSGTTYYLCATTAVRDIWGNANTATIASQAFVYNASTPRVVSALSGSPTSVLV
                     TFDQSMTNNAALTTRTNYTFAGCGALATNGGTTVTVVSGTQVLLAPLTTGTAGTCTLS
                     VNTAITSSAGVALTAATQSAIFSYSAATDTTAPAVASVQATNSNTVRVTFTEPINDST
                     VGTGDFSFSPSLTVTGVTCYTGAGNYTYCDVTTSTDQTTQNYSATVSGIQDVAGNNLP
                     SSTTTFTGDGKPYIVAIYPVDNGTVLVEWSEPVGNAASVGAGDYSISGATITGAALYP
                     AGTDPSRYVQLTINPTLTSGTSYTLSVNNPTGSVDSTGNGTLGTVPNGGTFTGPSATT
                     APQVTGGSSPSPTTLIVNFNEPLNNSTIATGDFALSGACNALTINSATQIQAGVVQLV
                     LAAGAVADGSCTVTVGAGNVSDLAGNAIAGTNNTATFSYKGTGTLDEIAPTVGSVVAL
                     SNTEIRVYFSEQVRNLGDANSGNNAANYSFSPSLTGGVQSVTCTATYCTLTLNAPGTA
                     SQQYSLTISNIQDTALTPNTMASTTVTFGGIGSSVTAPTLYQAVLINSTTVELSFSEQ
                     MALAAAQNTAHYTVSGGNTVSAAVLQADATKVRLTISPGAYGSSNSYTVTGTTGLTDV
                     SGVALGTPASATFNGSATAPAVTNLGDSSDLGVSNSDNITNPSVFPTPGLVFTGTVAA
                     NTTVYLYDDGVVVGTAVSDSSGNYTITLTSAGSTVTNGNNVFTVATVGPTGLVSDLST
                     PLTVNYDATAPGNPATAADLAAGSDTGFSTTDNITSDTTPTLSGSGLTPGDYVYIYDG
                     ATLIGAALVDGSGNWTWTLTPGDTTMAGAPADQQTYAAAGSGLSAGAHTITYKVGDTA
                     GNESSASPALNLTIDTSAPTLNLPVALTANSYIDLPFTGGGIYGSSSSASPVVLGNLT
                     ITLNSGTSGITISPTVTTTVGGALTGGETTIRVNLNVTGTPNGSETVQVVANSNAIFD
                     VAGNAVSFDTGVKNFAAAGVPNITGTPTYTSTGATTGYITVAWDEAPYTATSAAGVLT
                     ATDFDITFAKNAGGNANSMSITGFQDGTGGTLDAGETTMRILVSFDLATSGVETFRLN
                     AKSGEIFSGIAPYNNSPTTEQAGPFTAPDLLAPSAPASLDLAAADDTGSSNSDNITSQ
                     TTNLTISGTAEANSTVRLYQPNS"
     gene            complement(4390..6216)
                     /locus_tag="PLF85_09030"
     CDS             complement(4390..6216)
                     /locus_tag="PLF85_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE19850.1"
                     /translation="MKSRLFMPALALFFPALLNAAPPKTDVVLETTDAVLLHVEYVSE
                     NDKASVVDVRAAIVPPGICEAKEWPKYMPKNKFGNPTKMETGHCRFIGKPFVTRSLTL
                     APTDIKKRYDTALLTMSAGGEKKQLPFYDETGNAVRGFDFSADPIAEKGEAPVLGVRP
                     FAKDLSMDTMQTVDMGGTRSTLYPHHNLFHKWSKLDPRLGLYYSPLLPTGDISLLKFG
                     VIGVTVENSVAVPELKFLPLKKYSLRLRGGINAGFHRFSQDLFQNGQAVSEGQITLIP
                     VLAQLTLFWDLRPKGWGMTISPFVRLADGIIFSSVSTQVKPEYISLLAAGAESSRSGS
                     YIGHGFASAIGVEVRPDKWPVAFSFDGGYLMHSQDITGQYFMFNVGAAWHYGVTPPDP
                     KMIPIQYLGVKSVVLNLKGTVKNPDGSPIQNATLKLRAKGSTAVVKQVDTNEKGQYAM
                     EIEPGLDYAIEVHKDGYANANAELPLTTNDKKTQDRDFILAPLTYSLEGVNFKPDSDQ
                     LVTGKAPEKALLELVEFLKKNPEIRIEIGGHTAARGDDDPATIKLSERRADTVVKYLV
                     KKGIDNGRLTSKGYGGSKPIADGKTDAGAKKNRRVEVMILVK"
     gene            complement(6287..7456)
                     /locus_tag="PLF85_09035"
     CDS             complement(6287..7456)
                     /locus_tag="PLF85_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19851.1"
                     /translation="MLAHLELLGPMVVDALAQSLPCERSAIEAALAQLEADGSVFRLE
                     RGGETLFIERAYYQRLRHFHRRPGRDFVRVTPLRYLDFLVHWQRVADETRMQSAQALP
                     LVLRQLTGVALSRREWANTLRRRISDFQEKMLEDLLLTGEFFWYHPARPGSKVKNITL
                     QTRFMLLPRDFWFEVATPDLWPYPADLGADARRMYDYLERHGASFLADIHHSGKFFSE
                     QTTRAVRELIARGMVTSDQFFSLERLFQSRTRPGMAAPFPAGRFALVRVADSVDETRR
                     RRAIAEMLLRRQGVLYRYAAELDFFRVPWPELVRELRLMEMAGLIRSGRFVDLLWGEQ
                     FVEPHALPMLAAASPGAYKGGLEYDPVGIVHRLLARLGVVTRVLATPDSSAATAL"
BASE COUNT         1802 a         2113 c         1999 g         1620 t
ORIGIN      
        1 agctgtttgg ctggtaaagc cgaaccgttg aattcgcctc tgctgtaccc gaaatcgtca
       61 ggttggttgt ctgtgaagtg atgttgtccg aattcgatga acccgtatca tcagcggcag
      121 caagatcaag cgatgcgggt gcagaaggtg caagtaaatc aggcgccgta aaaggtcctg
      181 cctgctctgt tgtcggagaa ttattgtaag gcgctatgcc gctgaagatt tcccctgact
      241 ttgcattcag gcgaaatgtt tcaacgccgc tcgtcgcaag gtcaaagctg accaaaatac
      301 gcatcgtagt ttcaccggca tcaagagtac cgccggtgcc atcctggaag cccgtgatgc
      361 tcatcgaatt cgcgttacca ccggcattct tagcgaacgt aatatcaaaa tctgtcgcag
      421 tcagaacacc ggctgcagaa gtagccgtgt acggggcctc atcccaggcg acggtaatgt
      481 aacccgttgt agcccccgta gacgtatagg tcggagtacc ggtaatattc ggcacaccag
      541 ccgcagcaaa attcttcaca cccgtatcaa agctgacggc atttcccgct acgtcaaaga
      601 tcgcgttgct gttcgccaca acctgaacag tctctgaacc attcggcgtg ccggtaacat
      661 taagatttac ccggatcgtc gtctctcccc cggtaagcgc accacccact gttgtcgtca
      721 cagtcgggct gatagtaata ccactggtac ccgaattcag cgtaatcgta agattaccca
      781 gcacaaccgg agaagctgaa ctggaactgc cgtatatacc accgccggta aagggaagat
      841 caatgtacga atttgcggtc agggcgacag gaagattcag ggtcggagca ctcgtatcaa
      901 tcgtcaggtt caatgcaggc gatgctgaag actcattgcc tgcggtatcg cccaccttgt
      961 acgtgatcgt gtgcgcacca gcagagagac cactgccagc agcagcatac gtctgctggt
     1021 cagccggggc acccgccatc gtcgtatcac ccggggtcag cgtccacgtc cagttacccg
     1081 aaccgtcaac cagcgctgcg ccgatcagcg tcgcaccgtc atagatatag acatagtcgc
     1141 cgggagtcaa accagaaccc gaaagtgtcg gtgtcgtatc gctggtaata ttgtcggttg
     1201 tcgaaaagcc tgtgtctgaa cccgcagcaa gatcggcagc agttgccggg ttacccggtg
     1261 cagtcgcatc ataatttacc gtcaggggcg tcgaaaggtc actcaccagg cccgtcggac
     1321 caacagtcgc aacagtaaaa acgttgttgc cgttcgtcac cgtgctgccc gcgctcgtca
     1381 gagtgatcgt ataattgccg ctgctgtctg aaactgcggt accgacgaca actccgtcgt
     1441 cataaagata gactgtcgta ttcgctgcaa cagtaccggt gaacacaaga cccggtgtcg
     1501 ggaaaacaga agggttcgta atattgtctg aattcgaaac acccaaatct gaactgtcgc
     1561 ccaggttggt caccgcaggc gcggtcgctg aaccattgaa cgtggcagag gcaggtgtac
     1621 ccagtgcaac acccgaaacg tctgtcagtc ccgttgttcc tgtgacggta tagctgttcg
     1681 atgaaccata agcacccggt gaaattgtca ggcgcacctt cgtcgcatca gcctgcagca
     1741 ctgctgcaga aaccgtgtta ccgcctgaaa ccgtatagtg cgcggtattc tgcgccgcgg
     1801 ccagagccat ctgttcgctg aacgaaagct ccacagttgt cgaattgatc agaactgcct
     1861 ggtaaagcgt cggtgcagtc accgatgaac cgataccccc gaatgttaca gtagtcgatg
     1921 ccatagtatt tggcgtcaat gcagtgtcct gaatgttaga aatagtaaga gaatattgct
     1981 gcgaagcagt acccggcgca tttagcgtga gagtacaata tgtggccgtg caagtcacgc
     2041 tttgcacacc tccggtcagt gaaggcgaaa agctgtagtt cgcagcatta tttcctgaat
     2101 tcgcatcgcc cagattacgt acctgctcgc tgaaatacac ccgaatctcg gtattggaca
     2161 gagctacgac cgaacctacg gtgggcgcaa tttcatccaa tgtccctgtt cccttatagc
     2221 taaatgtagc agtattgttg gtaccagcta tcgcattacc ggcaagatct gatacattac
     2281 ctgcgccgac agtcacagta caactgccgt ccgccacggc acctgcggca agtaccagct
     2341 gcacgacacc ggcctgaatc tgtgtagcgc tgttgatcgt cagagcattg caggcaccgg
     2401 aaagagcgaa gtcacccgta gcaatcgtcg aattgttcag aggctcgttg aagttaacaa
     2461 tcagcgtcgt cggtgaaggc gatgaaccac cagtcacctg cggtgcagtc gtcgccgaag
     2521 gacccgtaaa cgtgccacca ttcggtaccg tacccagtgt gccgttgccc gtcgaatcaa
     2581 cgctgcccgt cgggttgttg acactcaggg tataagaagt gcccgatgtc agagtcgggt
     2641 tgatcgtcag ctgtacatag cgcgatggat cggtgccagc aggataaagt gctgcaccgg
     2701 tgatagtagc acccgaaatc gaatagtccc ctgccccaac agatgctgca ttgccaacag
     2761 gctctgacca ctctaccaga accgtgccat tatcaacagg ataaatcgcc acaatataag
     2821 gcttaccgtc gcccgtaaaa gtcgtcgttg aagatggcag gttattgccc gcaacatcct
     2881 ggatgccaga gaccgtcgcc gaataattct gcgtcgtctg gtcggttgat gtagtcacat
     2941 cgcagtatgt atagttgcct gcacccgtgt agcaggtcac acctgtcacc gtcagcgaag
     3001 gcgaaaaaga aaaatcaccc gtgcccacgg tcgaatcgtt gatcggttca gtaaaagtca
     3061 cacgcaccgt gttcgaattc gtcgcctgga cagaggcaac cgccggtgcg gtcgtatccg
     3121 tcgctgctga ataactaaaa attgccgact gcgtcgctgc ggtcagtgcc acaccagcag
     3181 aagaagtaat cgctgtgttc acgcttaacg tacacgtgcc cgccgtacca gtcgtcaaag
     3241 gtgcaagaag aacctgggta ccgctcacca cagtcacagt cgtaccgcca tttgttgcca
     3301 gtgcaccgca gcccgcaaaa gtatagttcg tccgcgtcgt cagcgcggcg ttgtttgtca
     3361 tcgactggtc aaaagtcacc agcactgatg ttggtgaacc tgacagggcg ctcacaacac
     3421 gtggcgtgct tgcattgtaa acaaatgcct gtgaagcaat cgtcgccgta ttcgcattac
     3481 cccagatatc gcgcacggct gttgtcgcac agaggtaata cgtcgtgccc gacgtcatca
     3541 ccgcagaagt cagtgtcacc tttgcgaagt tcactgaatc gcgcactgcg cccgtcacgt
     3601 tcacattgcc cgcagaacag ccgctattgg tataaatatc aaagttcgtg gcggtctctg
     3661 aactcgcctt ctctactgcc tctgtaaaat aaacctctac gctcgttgaa gacaaggcag
     3721 acaccgaacc aaatgatgga ccagtctggt ccgcgttcgt cggcgccgta aacgtagcag
     3781 agttggagct ccccaatgac gaaccatcga cacccacgat gcccgatgcc gttaccgtgt
     3841 acgttgtacc acccgtctgc gggatagtat ccacaaaaac cacccgtgag ttgttcgggt
     3901 cacgcgatgc ggcaaggata ttcagaagat tgccattaga atcagtaata cggtagttgc
     3961 tcaccagctg gcccgatgcc agggatacag gcttattgaa cgtcagcatc acccgcgtgt
     4021 tcgtcaaagg tgtcgccgtc agaagcgaaa cgttcaccga agaattgccc tgcgacccga
     4081 aaacaaggtc gaaaggctgg aaaaatgtgt tgtaagttcg accagagcag ttggccgcga
     4141 agaggcttaa ggctagcata cctccgcgga cgaaagtggt cattgctggt gaagttgtgc
     4201 gcatacgtat ccctgtgtac cctatcgggt tctgacgtac tctaccgtac tctttacgac
     4261 aaaaaatcag tgcagaaggg taaaatggaa gtgtgttctg ccagctgtat gcagcggggc
     4321 gcggaaatac ccgcgccact gactgagact gattatgtca ctggggagca ggagccccgc
     4381 ccgttaacct catttgacca gtatcatcac ctctacccgg cggttcttct tggcgccagc
     4441 atcggtctta ccgtcagcga tcggttttga accaccgtaa cctttcgatg ttaggcgacc
     4501 gttgtcgatg ccttttttca cgaggtattt gaccacggta tctgcccggc gttcagagag
     4561 cttgatagtc gccgggtcgt cgtcaccgcg ggccgcagtg tgacctccaa tctcaatacg
     4621 aatctcaggg tttttcttca ggaattccac aagttcgagc agcgcttttt ccggagcctt
     4681 gcctgtcaca agctggtctg aatcaggctt gaagttaact ccctcaagac tgtaggtcag
     4741 aggagcgaga atgaagtcgc ggtcttgcgt cttcttgtca ttcgtggtca aaggcagctc
     4801 tgcgttcgcg ttagcgtaac catctttgtg aacctcaatc gcataatcaa gacccggctc
     4861 aatctccatc gcgtactggc ccttctcgtt ggtgtcgacc tgtttgacga cagcagtact
     4921 acctttcgca cgcagcttca gcgtcgcatt ctgaatcgga ctgccgtcag gatttttgac
     4981 cgtacctttg aggttcagga ccacgctttt cacaccgagg tattgaatcg gaatcatttt
     5041 tgggtcaggc ggagttaccc cgtaatgcca ggcggcaccg acattgaaca tgaagtactg
     5101 cccggtaata tcctgtgaat gcatcaggta tccgccgtcg aaactgaagg cgaccggcca
     5161 cttgtcggga cgcacttcga caccgatagc gctggcgaaa ccatgcccga tgtaactgcc
     5221 agagcgacta ctctctgcac ctgctgcgag caatgagata tattcaggtt ttacctgcgt
     5281 gctgaccgag ctgaagatga taccatcggc gaggcggaca aaaggtgaaa tagtcatgcc
     5341 ccagcctttg ggtctgagat cccaaaaaag cgtcaattgc gccaacacgg gaatcagtgt
     5401 aatttgccct tcactgactg cctggccgtt ctggaagaga tcctgcgaaa agcgatggaa
     5461 acccgcattg ataccaccgc gcagccgcaa gctgtatttt ttaaggggca gaaacttgag
     5521 ttcaggtacc gcgacggaat tttccaccgt cacgcctata actccaaact tcagcagact
     5581 gatgtctccg gtcggcagaa gcggcgaata atagagaccc agccgcggat cgagtttgct
     5641 ccatttatga aagaggttat ggtgcggata cagagtcgaa cgcgtgccgc ccatatccac
     5701 agtctgcatc gtatccatcg aaagatcttt ggcaaaggga cgtaccccaa gcacgggggc
     5761 ttcgcccttt tcagcgatcg ggtcggccga aaaatcaaaa ccgcgcactg cattgcctgt
     5821 ctcatcgtag aacggcagct gttttttctc gccaccggcc gacatggtca aaagggctgt
     5881 atcataccgc tttttgatgt cggtgggggc aagcgttagc gagcgcgtca caaacggttt
     5941 gcctatgaaa cggcagtgcc cagtttccat tttggtcggg ttgccaaact tgttcttcgg
     6001 catgtatttc ggccattctt ttgcctcgca gattcccggc gggacaatcg ccgcccgcac
     6061 atcgaccaca gaggctttgt cattttctga cacatactcg acgtgcagaa gaaccgcgtc
     6121 ggtagtttcc aggaccacgt cggttttcgg tggcgcggca tttaacagcg ccggaaaaaa
     6181 cagggccagc gcaggcataa agaggcgtga tttcataact atgttgaatc tcgacgtcga
     6241 tacaacgata tttgcaacaa aaaaaacctg cccaacgcct ggtgaattac agcgcggtgg
     6301 cagctgaaga atctggcgtg gccagcaccc gtgtgacaac acccagtctc gcgagcagcc
     6361 ggtgcacaat gcccacggga tcgtattcta atcctccttt atatgcaccc ggagatgctg
     6421 cggccagcat tggcagtgcg tgtggctcga cgaactgctc accccagaga agatctacaa
     6481 agcgtcctga ccgaatcagg ccagccattt ccatcaggcg gagttcccgc acaagctcgg
     6541 gccagggcac gcggaaaaaa tccagctccg ccgcatagcg gtagagcacg ccctgtcgcc
     6601 gcagcagcat ctcagctatc gcacgccttc ggcgcgtttc atcgacagaa tcggcgacgc
     6661 gaaccagggc aaaccgcccc gcaggaaaag gtgcagccat accaggtctt gtccggctct
     6721 ggaacaggcg ctccaggctg aaaaactggt ctgaagtgac cataccgcgc gcgatcagtt
     6781 cgcgcacagc gcgcgtcgtc tgctcggaga aaaatttccc cgagtggtgt atgtctgcca
     6841 gaaacgaagc cccgtggcgt tcgagatagt cgtacatgcg ccgcgcatcg gcacccaggt
     6901 cagcaggata gggccaaaga tcgggcgtcg cgacttcgaa ccagaagtcg cgcggcagca
     6961 gcatgaaacg cgtctgcaaa gtgatgttct tcactttaga tcccgggcgc gcgggatggt
     7021 accagaaaaa ttcacccgtg agcaacaggt cttcgagcat cttctcctga aaatcagaga
     7081 tgcgccggcg cagggtattt gcccattcgc ggcgcgacaa cgcgaccccg gtgagttggc
     7141 gcagcacgag tggcagcgcc tgcgcagact gcatgcgcgt ctcatcagct acgcgctgcc
     7201 agtgcaccag gaaatcgagg tagcgcaaag gtgtcacgcg cacgaaatct ctgccggggc
     7261 ggcggtgaaa atggcgcagt cgctggtaat atgcgcgttc aataaacaag gtttcaccgc
     7321 cgcgctcgag acgaaagaca ctgccgtcgg cttcgagctg cgcgagggca gcttcaatcg
     7381 cggaacgttc acacggcaaa gactgcgcga gcgcatcgac caccatagga ccaagcagct
     7441 ccaaatgggc taacatgatt gacgcccggg cctgtgcacc tctgcgtgac gatacttcgt
     7501 caccgccgct ttcagcgaaa aacgcgagct gtac
//
LOCUS       DAOQJF010000148         7526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5367_length_7526_cov_4.554946, whole genome shotgun sequence.
ACCESSION   DAOQJF010000148 DAOQJF010000000
VERSION     DAOQJF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5367_length_7526_cov_4.554946"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1445
                     /locus_tag="PLF85_09040"
     CDS             102..1445
                     /locus_tag="PLF85_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19852.1"
                     /translation="MEVGLAILLFVCMTALLYVLLSLKITRQVDLQMKEFYKTRIHSD
                     IQEFYREMEGYAALMENRIQRFKSLVERSEALPDRGAQLQATEQPEIPQVHQSPMAER
                     ALNKKAALQKPVSPEKKRAAERVEKKVTKAAPVKAAKEKETKRPVTEPAKAVVVATPR
                     VRPVKQQPVPVAPAAAHDYSRQLSAAGPEAGIDADTTIAEELMKDLFAQDEMQLSRRV
                     NVAASSEPRRAQAPQPAKASAQVEPEATSVTGIFARIGKALEPVVFGERPQANAAAAK
                     PTVQPVQQQSATKAPALNTKTTDFAEVLRRAEQIKAEKKAERERAEIEARAEFYTAGD
                     EFVSKARPGLAAAGQAARNVSQPATGDAGTIRPAIRRESDLQQREMPARNSLAVKSLD
                     EHTVSFLIDSLKHDAGYRKQALRALTENNIPLEEIARLSRIDIGELQLMNQLGKF"
     gene            1589..3151
                     /locus_tag="PLF85_09045"
     CDS             1589..3151
                     /locus_tag="PLF85_09045"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNE19853.1"
                     /translation="MKIEAVRFGSQRSQAALLLFSAFLIAACGLVYELVAGTVSSYLL
                     GDSVTQFSRTIGVYLFAMGVGSYLSRFIVTDAVNKFIEIEIILALIGGLAAPLLFLAF
                     TVTEHYAWLLYSLLVIIGTLVGLEIPLLLRILKDRMNFRDLVARVLSLDYIGGLVAAV
                     GFPVLLLRPEVGLIRASLIAGIFNVLVAFVAIWVFQQQIRLRYHLVRACAVLLVLVSA
                     LVYSRPLQSFLDHELHTDPVVYSEQSHYQKIVITSWHEHFSLFLNNNLQFNSFDEYRY
                     HEALVHVPSQMFLDNRKDDRTPLRVLVMGGGDGLAVREFLRYPSLVRLDLVDIDPAMT
                     NLARNNAWLRQLNGASLSDPRVNVHHEDAFLFIRRPGLQYDVIVLDFPDPGNFAIGKL
                     YSDLFFKRLKKVMAPGAVAVTQSTSPFIARTAFWCIHNTVAAQGYQTLPYHTYVPSFG
                     EWGFVAFSRDRLAMPRRLAAGKSLRFLTSDVLASLVNFPADMAKVETGIQTLMTQNLV
                     HYYEKEWHRATR"
     gene            3120..4454
                     /locus_tag="PLF85_09050"
     CDS             3120..4454
                     /locus_tag="PLF85_09050"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNE19854.1"
                     /translation="MKKNGTGPHADRVAIHWFRRDLRLADNTALMAAAKSGLPVLPIF
                     IFDKNILSRLRLKEDRRIAFIYETLVKLRESLLQHGGQLLVLHGKPEEVFASLAERFS
                     IAAVYCNEDYEPYARERDAKVAKFCQERGIVFTSTKDHVVFAPHEVLKDDGTPYHVFT
                     PYSRRWLAAFEQNRPVVQAGLPQCRWQPAEGDNAMPRLEELGFYPVSSLAPPALLDSA
                     IIENYAATRDIPGIRGTTRLGIHLRFGTVSVRQLALVAQELSTKYLVELIWREFYQMI
                     LWYYPDTMSKAFRAQYAGLKFPGTEDDFRRWRNAETGYDLVDAGIRELVETGFMHNRV
                     RMVVASFLTKHLLCDWRWGEHFFAQYLLDYELASNVGGWQWSAGIGVDAAPYFRIFNP
                     AEQQKKYDADGAYVQRWLGTDSNFFGTPRPQPIVDHRFARERCLKFFSTARP"
     gene            4493..6655
                     /locus_tag="PLF85_09055"
     CDS             4493..6655
                     /locus_tag="PLF85_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE19855.1"
                     /translation="MSWAVAAIIAITTIHAADGKYPVTPGEIADIHNLGAGINTPDSE
                     YTPFITPDERFLFFQSNREGAVGPEGDFDLWYSMNKNAGKAEDPLFETSANVGLPINS
                     ESLDGHPSLRKLPTGEYEMYFSSFSSATRQGPQLTNIYYTVWRGGKWTVPQPVAELNS
                     EYHDRMPSISQDGRYMFFSSDRPGGRGGDDIWFSELDMATGKWTPPQNAGNINTSASE
                     VTPAIHSDGITLYYSSDRAGGVGGYDIYFTQSVARLENPEETKMLAQGWANPLNLGKP
                     FNSEFDDEYPTVTANGQRVYFTSNRVEGRGAFDIYRARVPDFARPTARMLFTGKTLIA
                     GSKRPLAAAVKLTAGPMSSGIETDAAKGASFEFTIINQRVYNAEAQAPGFKVAREEFD
                     TRGLHDGKGIERDLLLLRDIKIPKTVALKLTFLDGEGKKVSPAAKVKVAPRDKKASKF
                     KPGSLISLTDLADFGGDEEKALLALESMVIDVAATKKGYQPLKDKILVSALLDKYTGE
                     LPPVIDLTIALTRKGEAVPARSEVPAVKEEAPLREEKQQVAARNYLKFIGRIYFRSND
                     AEKPLAGASTTVKKAAQAWKKNPSRRLFVYGHGDSKGGRTYNLKLSKARGNLIRKQLI
                     ELGVTARKITVQGAGKSQPRVRNDNTEAKRQKNRRVEIYITEPLAEPAGEQGEGKAEA
                     KPAESSVPAQPETKPAAEPEATGPANPASEPTGETPQQ"
     gene            complement(6688..>7526)
                     /locus_tag="PLF85_09060"
     CDS             complement(6688..>7526)
                     /locus_tag="PLF85_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNE19856.1"
                     /translation="FSHTVFALPFALAALAVIAARHSEKVALTWQKGVLVVVAFTAMR
                     SFAMAVNRYADRKIDALNPRTAQREIPAGILSPRTVLLFALATLVTLVVCAWLLSPLA
                     GYLALPAAVLVATYSIAKRFTALCHFWLGLAIGMAPSAVYIALLQQVFPEALLMSAAL
                     AFYIAGFDILYALQDREFDRKAGLYSIPARYGEQTALWISRLSHVLSLVMIFFVLQQL
                     GVSSSGIAGLCLLGALLALEHWLVGKADAIRYEKIPIAFFNVNSVFSVCFLLVICAAL
                     WL"
BASE COUNT         1723 a         2097 c         2141 g         1565 t
ORIGIN      
        1 aaaggacttc tgtaatttaa acgattgccc cgcgggggca atcgtcccgg tgatgtgaca
       61 taattcttga cagagcgaaa agtcagcgac gcataggccc tatggaagtc ggtctagcga
      121 tactgctttt cgtctgcatg accgcattgc tctacgtgct gctctcgctg aagatcaccc
      181 gccaggttga tctgcagatg aaggagtttt acaaaacacg tatccattcc gatatccagg
      241 aattttaccg cgagatggaa ggatatgcgg ccctcatgga aaaccgtatc cagcgcttta
      301 agtcgctggt cgagcgcagc gaggcgttac ccgatcgcgg tgcgcagctg caggcgacag
      361 aacaaccgga aatcccacag gttcaccagt cgcccatggc ggagcgtgcg ctgaacaaaa
      421 aagcagcgct gcaaaagccg gtatcccctg aaaagaaaag agcggccgag cgcgtggaga
      481 aaaaagtcac aaaggctgcc ccggtgaaag ccgccaaaga aaaagaaacg aaacgaccgg
      541 tcactgagcc agcgaaggct gtggtcgtag ccacgccacg cgttcggccc gtgaagcagc
      601 agcctgtgcc ggtggcgcca gctgcagcgc acgattacag ccggcagctt tctgccgccg
      661 ggcctgaagc gggtatcgat gcagacacga ccatcgccga agagttgatg aaagacctgt
      721 tcgcgcagga tgaaatgcag ctttcgcgcc gtgtcaacgt ggccgcatcg agtgaacctc
      781 gacgcgcgca agcaccacaa ccagcgaaag cttcagcaca ggttgaacct gaagcgacat
      841 ccgtcacagg catttttgcc cgcatcggta aggcgctcga acctgtagtc ttcggcgaac
      901 ggccgcaagc gaatgctgcc gcagcgaagc ccacagtgca gccggttcag cagcagtctg
      961 caaccaaggc gcctgcgctt aatacaaaga ccacggattt tgccgaggtc ttgcgccgcg
     1021 cagagcagat caaggcagaa aaaaaagccg agcgcgaacg cgcagagatt gaagcgcgcg
     1081 ctgaattcta cactgccgga gatgaattcg tttccaaggc ccggccgggc cttgcagcgg
     1141 caggacaggc agcacggaat gtatcgcagc ctgctacagg tgatgcaggc acgataagac
     1201 ctgctatccg gcgcgagagc gatctgcagc aacgcgagat gcctgcgcgc aacagcctcg
     1261 cggtgaaaag cctcgacgag cacaccgtca gtttcctgat cgattcgctc aaacacgacg
     1321 cgggttaccg caagcaggca ctgcgcgcgc tgaccgagaa taatattccg ctcgaagaga
     1381 ttgcgcggct cagtcgcatc gatattggcg aattgcagct gatgaaccag ctgggtaaat
     1441 tctaagtggg cacctcgaaa aataccgcca gaaaatcgga aacgctgcgg attacaggca
     1501 aaccgcacgc aaaaggccga agcggcctgt ttccaatttt ctgcgtcgcc gtgcgagcgt
     1561 cgtttgcggc attttccgag gtgccccgtt gaaaatcgaa gctgtgcgct ttggcagcca
     1621 gcgctcgcag gcggccctgc tgctcttttc tgccttcctg atcgcagcct gtggcctcgt
     1681 ttacgaactc gttgcgggca ccgtttcgag ttacctgctc ggcgatagcg tgacgcagtt
     1741 ttcgcggacg atcggcgtct acctgtttgc gatgggcgtg ggctcgtacc tcagccggtt
     1801 tatcgtcacc gacgcggtga acaagtttat cgagattgaa atcattcttg ccctgatcgg
     1861 tgggctggcc gcacccctgc ttttcctggc gttcacggtg acggagcact atgcctggct
     1921 gctctattcg ctgctggtaa tcattggtac gctggtgggt cttgagatac ccctcttgct
     1981 gcgcatactc aaagaccgta tgaatttccg cgacctcgtg gcgcgcgtgc tctcgctcga
     2041 ctacattggc ggccttgtgg cggctgttgg ttttcctgtg ctgttgctgc ggcctgaagt
     2101 gggtctcatc cgcgcgtcgc tgattgcggg tatattcaat gtgctggtgg cctttgtggc
     2161 gatttgggtt ttccagcagc agattcgttt gcgctaccac cttgtcaggg cctgcgcggt
     2221 gctgctggtc ctggtttcgg cgctggtgta ctcgcgcccg ctgcagagtt ttctcgacca
     2281 cgagctgcat accgacccgg ttgtctattc agagcagtcg cactaccaga agattgtcat
     2341 tacctcgtgg cacgaacact tctctctctt cctgaataac aacctgcagt tcaatagctt
     2401 tgatgaatac cgctaccacg aagctctcgt gcacgtgccc tcacagatgt ttctcgataa
     2461 ccggaaggac gacaggacgc cgctgcgggt actggtgatg gggggcggtg acggccttgc
     2521 agtgcgggag ttcctgcgct atccgtcgct ggtccggctc gacctggtgg atatcgaccc
     2581 ggcaatgaca aatctcgcga ggaacaatgc ctggctgcgg cagctaaacg gcgcttcact
     2641 cagtgaccct cgcgtgaacg tccaccatga agatgctttt ttgtttatcc ggcggccggg
     2701 gttgcagtat gatgtcatcg tgctcgattt tcctgacccc ggaaattttg ccattggtaa
     2761 gctgtattct gacctgttct ttaagcggct gaagaaggtt atggcaccgg gggcagtggc
     2821 tgtcacacag tcgacatccc cctttattgc ccgcaccgca ttctggtgca tacataatac
     2881 tgtcgcagcg cagggttacc aaaccctgcc gtaccatacc tacgtgccga gtttcggcga
     2941 atgggggttt gttgcattct cgcgtgacag gctggcgatg ccccggcggc tcgcggccgg
     3001 taaaagcctg cgctttctga cttccgacgt acttgcgagc ctggtaaatt ttccggcgga
     3061 catggcaaaa gttgaaactg gtatacagac cctcatgacc caaaacctcg tgcactacta
     3121 tgaaaaagaa tggcacaggg ccacacgctg accgtgtagc gatccactgg tttcggcgcg
     3181 atctgcgcct tgcggacaat accgcgttga tggcagcagc gaaaagcggc ctgccggttc
     3241 tgccaatctt catattcgac aagaatattc tttcgcggct gcggctgaaa gaagaccgcc
     3301 gcatcgcctt catatatgaa accctggtaa agctgcgcga gtcattattg cagcacggcg
     3361 gccagttatt ggttttgcac ggtaaacccg aagaagtatt cgcttcgctc gccgaacgat
     3421 ttagcatcgc agcagtatac tgcaacgaag actatgaacc gtatgctcgc gagcgcgatg
     3481 cgaaggtggc caaattctgc caggagcgcg gtattgtatt cacctcaaca aaagatcacg
     3541 tggtgtttgc gccgcacgag gttctcaaag acgacggtac tccttaccat gtttttacac
     3601 cttactcgcg ccgctggctt gcggcgtttg agcagaatcg gccggtagta caggccggtt
     3661 tgccgcaatg ccgctggcag ccggctgaag gcgacaacgc gatgccgcgc ctcgaagaac
     3721 ttggatttta tcccgtctct tcactggcac cgccggcgct gctcgacagt gccatcattg
     3781 agaactacgc ggcgacacgt gatattcccg gtattcgcgg tacaacacgc cttggcattc
     3841 atctccgctt cggtacggtc tctgtccggc agttggcgct tgtcgcacaa gaactgagca
     3901 cgaagtatct ggtagagctc atctggcgtg aattttacca aatgatttta tggtattatc
     3961 ccgatacgat gagcaaggcg tttcgtgcac agtacgctgg cttaaaattt cccggcaccg
     4021 aagacgactt caggcgctgg cgcaatgcag aaaccgggta tgacctcgtc gatgccggca
     4081 ttcgcgaact ggtcgagacc ggtttcatgc ataaccgggt gcgtatggtc gttgcctcgt
     4141 ttctgaccaa gcacctgctg tgcgactggc gctggggaga acattttttc gcgcagtacc
     4201 tgcttgacta tgaactcgct tcgaacgttg gtggctggca gtggagcgca ggtatcggcg
     4261 ttgatgctgc cccgtatttc cgtatcttta acccggccga gcagcagaag aaatacgatg
     4321 ccgatggtgc ttacgtccag cgctggctgg gcaccgattc gaattttttt ggcactcctc
     4381 gtccacaacc catagtcgat catcgctttg cgcgcgaacg ctgcctcaag tttttctcca
     4441 cagcacgtcc ttagttttga ctcgggataa aggggaacaa atgttcaatt ttttgtcttg
     4501 ggccgttgct gccattatag caatcactac aatccacgcc gcggatggca aatacccggt
     4561 aactccgggc gaaatcgcgg atatccataa cctgggcgcg ggaattaata ccccggatag
     4621 cgagtatacg cccttcatca cgcccgacga gcgtttcctc tttttccagt caaaccgcga
     4681 gggggcggtc ggtcctgaag gcgattttga tctttggtac tcgatgaaca agaatgccgg
     4741 caaggcagaa gatcctcttt ttgagacgtc ggccaatgtt ggtctcccga tcaactccga
     4801 gtcgctcgac ggacatccct ctctgcgcaa gctgccaacg ggggagtacg aaatgtactt
     4861 ctcttctttc tcgtctgcca cgcgccaggg gccacaactg acgaacatct attacaccgt
     4921 ctggcgcggc ggtaaatgga cagtaccaca accggtcgcc gagttgaatt ctgaatacca
     4981 cgaccgtatg ccttccattt cgcaggatgg tcgctacatg ttcttctctt cggaccgccc
     5041 gggtggtcgc ggcggcgacg atatctggtt cagcgagctg gatatggcga ccggcaagtg
     5101 gactccgccg cagaatgccg gtaatatcaa tacctcggcg tctgaagtaa cgcccgcgat
     5161 ccactctgac ggtatcactc tctactattc gtctgatcga gccggcggtg tgggaggtta
     5221 tgacatctac tttacccagt cggtcgcgcg tctcgaaaac cccgaagaga ctaaaatgct
     5281 cgcccaaggc tgggcgaatc cgctcaatct tggcaaaccc ttcaattcag aatttgacga
     5341 cgaatacccg acggtgacag ccaatggcca gcgtgtgtac ttcacttcaa accgggttga
     5401 aggccgcggt gcgtttgata tttaccgcgc ccgggtgccc gattttgccc ggccaactgc
     5461 gcggatgctt tttaccggca aaacgctcat agcgggttca aagcggccgc tggctgcagc
     5521 ggtaaagctt acagccggcc ctatgagttc gggtatagaa accgacgctg ctaaaggagc
     5581 gtcttttgaa tttaccatca tcaaccagcg cgtttacaat gcagaggcgc aggcgccggg
     5641 gtttaaagtc gcgcgcgaag aattcgatac gcgcgggctg cacgacggta agggaataga
     5701 gcgtgacctg cttctgctca gggacattaa gattccgaag actgtcgcgc tgaagctcac
     5761 gtttctcgat ggtgagggca agaaggtgtc gcctgcagca aaagtaaagg ttgctccgcg
     5821 cgacaaaaaa gcttcgaaat tcaaacccgg gtcgctgata tctctaaccg acctcgcaga
     5881 ttttggcggt gatgaagaaa aagcactgct ggcgctggaa tcaatggtta ttgacgttgc
     5941 cgccacgaaa aagggctacc agccgctcaa agacaagata ctggtgagcg cattgctcga
     6001 taagtatacg ggtgagttgc cgcctgttat tgacctgacc atagcgctta cgcgtaaagg
     6061 cgaagcagta ccagccagaa gtgaagtgcc cgcggttaaa gaggaagctc cgctgagaga
     6121 agaaaaacaa caggtcgctg cgcgcaacta cctgaagttt atcggccgta tctacttcag
     6181 aagtaacgat gcagaaaagc ccctggcggg agccagtacg accgtgaaga aagcagcaca
     6241 ggcctggaaa aagaacccca gccgcagatt gtttgtctat ggtcacggtg acagcaaggg
     6301 tgggcgtacc tataacctga aactatcaaa ggcgcgcggc aacctgatca gaaagcagct
     6361 tatcgagctg ggtgtaaccg cgcgcaagat caccgttcag ggggcgggca agtcgcagcc
     6421 gcgtgtgcgc aatgacaaca ccgaggcgaa gcggcagaaa aaccgcaggg tagaaatcta
     6481 catcaccgaa ccgcttgccg aaccagcggg cgaacaaggt gaaggtaaag ccgaggcaaa
     6541 gcctgctgaa agcagtgtcc cggcgcagcc agagaccaaa ccagcagcgg aacctgaggc
     6601 aactggcccg gcgaacccgg ccagcgaacc cacaggcgaa accccgcagc agtaaggtaa
     6661 tcgggcttta gcaggttccg gaacctgcta aagccacagg gcggcgcaga ttaccagcag
     6721 aaagcataca ctgaatacgc tgtttacgtt aaaaaaggca atcggtatct tttcataacg
     6781 gatagcatcg gctttaccga caagccaatg ctcaagggcc agcagcgcac cgagcaggca
     6841 caggccggcg atgccgctgc tactcacgcc gagctgttgc agcacgaaga aaatcatcac
     6901 cagcgacagc acgtgagaca gccgcgatat ccagagcgct gtctgttcgc cgtagcgcgc
     6961 cggaatcgaa taaagcccgg ccttgcggtc gaattcgcgg tcctgcagcg catagagaat
     7021 atcaaacccc gcaatataga atgccagggc agctgacatc agaagcgcct caggaaaaac
     7081 ctgctgcagc agggcaatgt atactgccga aggcgccatg ccgatagcga gtcccagcca
     7141 gaaatggcag agcgcggtga aacgtttagc gatactgtaa gtggcgacga gtacggcggc
     7201 aggcagggcg aggtatcctg ccagcggcga gagcagccag gcgcacacga ccagcgttac
     7261 gagtgtggcc agggcaaaaa gcagcacagt gcgcggtgac aggattcccg cgggaatttc
     7321 gcgttgcgct gtgcgcgggt tcagcgcatc gatcttcctg tctgcgtagc ggtttaccgc
     7381 catcgcgaag ctgcgcatcg cggtgaaggc aacgactacc agcactccct tctgccatgt
     7441 cagtgccacc ttttcgctgt ggcgcgcagc aatcacggcg agtgcagcga gcgcgaaggg
     7501 cagggcgaat acggtgtgtg agaacc
//
LOCUS       DAOQJF010000149         7468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5428_length_7468_cov_6.162687, whole genome shotgun sequence.
ACCESSION   DAOQJF010000149 DAOQJF010000000
VERSION     DAOQJF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5428_length_7468_cov_6.162687"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1169
                     /gene="rfbA"
                     /locus_tag="PLF85_09065"
     CDS             282..1169
                     /gene="rfbA"
                     /locus_tag="PLF85_09065"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNE19857.1"
                     /translation="MKRKGIILAGGSGSRLHPATLAISKQLLPVFDKPMIYYPLSTLM
                     LAGIRDILIISTPQDTPRFEQLLGDGSRWGIRLHYAVQFSPDGLAQAFIIGESFIGDD
                     LSALVLGDNIFYGHDFHHLLANAMARDEGASVFAYHVKDPERYGVAEFDSCGKVLSLE
                     EKPRQAKSNYAVTGLYFYDRQVVELARNLQPSPRGELEITDLNRLYLEQGKLKVEIMG
                     RGYAWLDTGTHESLLDASQFIATLENRQGLKVACPEEIAYRQRWIDAEQLVNLAQPMA
                     RNGYGQYLLRLLNEKNLAG"
     gene            1270..1815
                     /gene="rfbC"
                     /locus_tag="PLF85_09070"
     CDS             1270..1815
                     /gene="rfbC"
                     /locus_tag="PLF85_09070"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNE19858.1"
                     /translation="MKATALAIPHVMLLEPKVFGDDRGFFFESFNQAQIETALGRKVD
                     FVQDNHSRSAKNVLRGLHYQIKQPQAKLVRVVLGEVFDVAVDIRKSSHTFGQWVGQIL
                     SAENKRQMWIPEGFAHGFVVLSDTAEFLYKTTDYWAPEHERCIVWNDTNLAINWHLQG
                     EPVLSTKDAQGVALHKAELFA"
     gene            1985..2815
                     /locus_tag="PLF85_09075"
     CDS             1985..2815
                     /locus_tag="PLF85_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE19859.1"
                     /translation="MNPHQRHPATPSAMVLSLWHNRQLIIQMTRREVIGRYRGSIMGL
                     AWSFFNPVLLLLVYTFVFSIVFKARWGTGAEESRADFAILLFIGMIIHGVFSECVNRA
                     PSLIVGNVSYVKKVVFPLQILPLVALGSALFHAAVSIVVLLVAKLLVHGSVPWTLLFL
                     PMALLPLLIGTLGIAWVLAALGVFIRDIGQITSLLTTVLLFISPVFYPISSLPAKYQQ
                     ILLLNPLTFVIEESRRIMFFGTVPDFQGFLLATGVSLLMSWAGFWWFQRTRKGFADVL
                     "
     gene            2805..4250
                     /locus_tag="PLF85_09080"
     CDS             2805..4250
                     /locus_tag="PLF85_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE19860.1"
                     /translation="MSFDDPVIRVNKLSKCYQIYDKPQDRLKQAIMPRLWRLTGKTEQ
                     PYYREFWALNNVSFEVRRGETMGVIGRNGSGKSTLLQILCGILTPSSGSVEIAGRVAA
                     LLELGSGFNPEFTGRENVFLNASVLGLTDEEIRARFDDIVEFSEVGEFIDQPVKTYSS
                     GMYVRLAFAVIAHVDADVLIIDEALSVGDVFFVQKCMRFLRKFMERGTVLFVSHDSAA
                     VINLCHTALWLSHGEVVSHGEPKAVSEKYLENLARSAIGIDHQPGSRSVQPAATLQQV
                     RRGIPRDMRQDFLNSSSLRNDIQIFAFVEDAHSFGAGGATITGVCLTDRNAQPLNWVV
                     GGEDVCLIISGVAHQGIIGAIAGFHLKDRLGQVLFGDNTFLRYLDTPVRLHGGQCFEA
                     RFAFRMPILPAGDYTVGVAFAEGSQAQHVQHHWIHDALAVHSISSSVATGLIGIPMEE
                     IVLEVSACAVSGLPGNDHDNSVSAAGNGEGK"
     gene            4247..7456
                     /locus_tag="PLF85_09085"
     CDS             4247..7456
                     /locus_tag="PLF85_09085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE19861.1"
                     /translation="MKFTGERFIPTEHGEISLEHYHRYAATSEYVKDKVVLDVACGEG
                     YGSDLMALGAASVVGVDLSAEAVEHAKRTYAERGNLRFLQASAIALPFPDSSFDVVVS
                     FETIEHLAEQEQMLCELRRVLRPAGILMISSPNRPVYSEESGQHNEFHVKELDFSEFD
                     SLLRSKFSRIRYFGQRFMMGSVLQPLEGGGKHFDALCEHKGGIRNSITPLQYPVYFIA
                     VCGDEASAFPEVRSSFLLPRELDLLRQYQGYAKWAVRVDAELATCRELYSNLDEEHHR
                     VANWAKSLYQRCELLEREVAETEAKLSATQAKLDQSIRSGSSSVARLSPFAAGLARGQ
                     WTTAMKPLRTWLRTWLRTWVRSQGKRLYHQLPLSQTFKLKLVGGMYKFAGPLFAGMSH
                     YETWQHQRNGVPLPMAMQGAVKLDEIDSVLQELSFELPEHPLVSIVIPAYGNLPVTLG
                     CLRSIARNLPAVPVEVILMEDCSGDPEINRLSEVLGLRYLRNPQNLGFLRSANRAADF
                     AQGNYFYLLNSDTEVTEGWLDAMLAVFKSHPDCGLVGSKLVYPDGRLQEAGGIVWNDA
                     SAWNYGRFQDPSLPEFNYLRETDYCSGASIMIPRELYKSLGGFDERYVPAYFEDTDLA
                     FAVRSAGRKVYYQPASVVIHYEGVSHGTDTGEGVKSYQVQNQQKFREKWRNVLERDHF
                     QNAHHLFQARDRSRSKKAVLVIDHYVPRPDRDAGSRSMLAIMQELLAMGFNVKFWPHN
                     HWQDPVYTPRLQALGIEVEYGSRYWGRFATWIEENGKYLDYVLLSRPDVAADFVQALR
                     KYTKLPLIYYGHDIHHLRLKEQLKLEPENHTVRNEMKRIEKIEKQHWRQMDAIYYPSN
                     LETEEVAKFLKSEGLEDKSVTLPVYAFDSFSYNAAVNLADRHDILFVAGFGHPPNATA
                     ARWFVHEVLPLVRESFPDVRLTLVGSNPTPEVLALACADIAVTGFVTDEQLAEYYLNA
                     RVAVAPLLFGAGMKGKVVEAMRFGIPMVTTPIGAQGLVEAGSALGVFEDPSAYAKDIT
                     RLLSDDEEWRMRSAAELAYVQEHFSLAAMRFSLERWFVP"
BASE COUNT         1621 a         1991 c         2048 g         1808 t
ORIGIN      
        1 aggggatgcc gttggcgtca agaagccccc tgtggattgc tttcccagtg tcgttattca
       61 tttatggcag atttccaggt gattgccgag tgagcccgtc tggccggaga gggtgaattc
      121 tatcttgatc ccctcgctgt ggtgaaattg ccagtttttt tcggggggtc cgaccgccaa
      181 ttttggcaac ttctccgctc cgcgtactgt gtctggacgc tcccaagtgg tagactcgca
      241 ggccattcca ctgatgatcc gtcttgactg gaaatatctg catgaaacgt aaaggcatca
      301 ttcttgctgg tggttctggc tcccggcttc acccagccac cctggcaatc agcaagcagt
      361 tgctcccggt attcgacaag ccgatgatct attatccgct cagcaccctc atgctggcgg
      421 gaatacgtga catcctgatc atctcgacgc cacaggacac gccacgcttc gagcaactgc
      481 tcggtgacgg ttcgcgttgg ggtatccgtc ttcactatgc tgttcagttc agtcccgacg
      541 gtctggcgca agcgttcatc atcggggagt cgttcattgg cgatgacctg tcggccctgg
      601 tgctgggcga caacatcttc tacggccatg acttccacca cctcctggcg aatgccatgg
      661 ctcgtgacga gggagcaagc gtctttgcct accatgtgaa agaccccgag cgttacggag
      721 tggccgagtt tgattcctgc ggcaaggtgc tatcgctgga agaaaaacca aggcaagcaa
      781 aatccaatta cgccgtcacc ggtttgtatt tttatgatcg acaagtagtc gagctagcca
      841 ggaacctgca accctcgccg cgtggcgaac tcgagatcac cgatctcaac cgcctgtatc
      901 tggaacaggg gaaactcaag gtcgaaatca tgggacgggg ctatgcctgg ctggacaccg
      961 gtacccacga atcgctgctc gatgccagcc agtttattgc cacgctggaa aaccgccagg
     1021 gactcaaggt ggcctgcccg gaagaaatcg cttatcgaca gcgatggatt gatgccgagc
     1081 aactggtcaa cctggcgcag ccaatggcca ggaacggcta cgggcaatac ctgctgcgcc
     1141 tactgaacga aaaaaatctg gccggttgaa gcggattgtt ccggtcgccg ctaccaacgc
     1201 cccttgtcgc ggcaccgtcg accactgtga acttgccaca tacccatcac gaaaggcagc
     1261 ccagacgcaa tgaaagcaac cgctttggct atcccccatg tcatgttact cgaacccaag
     1321 gtattcggag acgaccgggg ctttttcttc gagagcttca accaggcaca aatcgaaacc
     1381 gcgcttggca ggaaagtcga tttcgtgcaa gacaatcact cccgctcggc aaagaacgta
     1441 ctgcgtggtc tgcattatca aatcaagcag cctcaagcca aactggtacg cgttgtgctt
     1501 ggcgaggttt ttgacgtggc ggtggatatc cgcaagtcct cacacacctt tggtcaatgg
     1561 gtgggccaaa tcctgagtgc agaaaacaag agacagatgt ggatccccga gggctttgcc
     1621 cacggcttcg tcgtgctcag cgatacagcc gaattccttt ataagaccac tgactactgg
     1681 gcgccggagc acgagcgctg catcgtctgg aatgacacca atctggcgat caactggcac
     1741 ctccagggtg aaccggtgct ttccacgaaa gacgcgcaag gagtggcact acataaagct
     1801 gaactctttg cgtgataggg cacttcgccg tctctccact cgttgcacga ccaagcaatc
     1861 gccgggatag ttcatcgaat tacgatgcgc atcacgcgtt gccggttctc gccggtgaac
     1921 cagtctctca accaggaata ccacccttga gcgccgacaa ccattgacag ccattatata
     1981 gattatgaat cctcatcaaa gacatcccgc cacgccatcg gcgatggtac tgagcctgtg
     2041 gcataaccga caactcatca tccagatgac tcgtcgagaa gtcattggcc ggtatcgtgg
     2101 ctcgatcatg ggcttggcgt ggtctttctt caatccagta ttgctgctcc tcgtctacac
     2161 tttcgtcttt tccattgttt tcaaagcccg atggggaact ggcgctgagg aaagccgcgc
     2221 agatttcgcc atcctgctgt tcatcgggat gatcatccat ggcgtgttct cagaatgtgt
     2281 caatcgggct ccgtctctga ttgtcggcaa tgtaagttat gtcaaaaagg tggtcttccc
     2341 gctgcaaata ctacctttgg tggccctggg ctcagccctt ttccatgcgg ctgtcagtat
     2401 tgtagtgttg ctcgttgcaa agttgctcgt ccatggttct gtaccctgga cgctgctctt
     2461 tctaccgatg gcactgcttc ctctgctgat cggcaccttg ggaatcgcct gggtacttgc
     2521 agcgcttggc gtcttcattc gtgatattgg gcagatcacc tcattattga ccaccgtcct
     2581 gctgttcatc tcgccggtgt tctacccgat ctccagtctg cctgcgaagt atcagcaaat
     2641 tctgttgctc aaccctctga ctttcgtgat cgaggaaagt cgcaggatca tgtttttcgg
     2701 taccgttcca gatttccagg ggttcctgct ggctaccggt gtttccctac tgatgtcttg
     2761 ggcagggttc tggtggttcc agcggacacg gaaaggcttc gctgatgtcc tttgacgatc
     2821 ccgtcattcg ggtcaataag ctgtccaagt gctatcagat ctacgataaa ccacaggaca
     2881 gactcaagca ggccattatg cctcgtttgt ggcgcctgac cgggaagacg gaacaaccct
     2941 attaccggga attctgggcg ctgaacaatg tttcctttga ggtccggcgc ggcgagacca
     3001 tgggagttat cggccgtaat ggttcaggaa aatcgacgct gctgcagata ctgtgcggca
     3061 tcctgacgcc tagttcgggt tcggtagaga ttgccggtcg agtagctgcc cttcttgaac
     3121 tgggttccgg cttcaatccg gaattcaccg gacgggaaaa tgtttttctc aacgcatccg
     3181 tactgggtct cactgacgag gagatacgcg cccgttttga tgacattgtc gaattctcgg
     3241 aggttggcga gttcatcgat caacccgtga aaacctattc aagtggaatg tacgttcgtt
     3301 tggccttcgc cgtcattgcg catgtcgatg ccgacgtact catcatcgac gaggcgttgt
     3361 cggtcgggga tgtcttcttc gtccagaaat gcatgcgctt cctgcgcaag ttcatggagc
     3421 gtggaaccgt cctgttcgtc agtcacgatt cagctgccgt aattaacctg tgccacaccg
     3481 ccctctggct gagccacggc gaagtggtga gccatgggga gcccaaagcg gtttccgaaa
     3541 aatacctgga aaacctggcc aggtcagcca ttggcattga ccatcagccg gggtcacggt
     3601 ctgtgcagcc tgccgcgaca ttgcagcaag tgcggcgggg catcccaagg gacatgcgcc
     3661 aggatttcct gaatagctca agcctgcgca acgatatcca aattttcgcg ttcgttgaag
     3721 acgcacacag ctttggtgcc ggtggcgcca cgattaccgg agtctgcctg acggacagga
     3781 atgctcagcc gctgaattgg gtggtgggtg gagaggacgt ctgtctgatc atttctggtg
     3841 tggcacatca gggaattatc ggtgcaattg ccggtttcca tttgaaggac aggctgggac
     3901 aggttctgtt tggcgacaat acttttttac gttacctgga tacgcctgtg aggcttcacg
     3961 gcgggcaatg tttcgaggca cgatttgcct tccgtatgcc cattctgccc gctggtgact
     4021 acacggtcgg tgtcgctttt gctgaaggca gccaggccca gcatgtgcag catcactgga
     4081 tacacgacgc tctggcggtt cactccatca gttccagcgt agcaaccggt ctgatcggca
     4141 ttcccatgga agagattgtt ctggaggtat cggcatgcgc cgtttccgga ttgccaggca
     4201 atgatcatga caattcggtg tctgcggcag gaaacgggga gggcaaatga aatttaccgg
     4261 tgaacgcttc atccctacgg aacacgggga aatctcgttg gaacactacc atcgctatgc
     4321 ggctaccagc gagtacgtga aggacaaggt ggttctcgat gtcgcctgcg gtgaaggcta
     4381 tggctcggat ctgatggcat tgggggcggc aagtgtcgtt ggcgtagacc tgtctgccga
     4441 ggctgtggag cacgccaaac ggacgtatgc cgaacgggga aatcttagat ttctgcaggc
     4501 cagcgctatc gccctgccat ttcccgacag cagtttcgac gtcgttgtct cgtttgagac
     4561 catcgagcat ctggccgaac aggagcagat gctgtgtgaa ctgcgaaggg tgcttcgccc
     4621 ggcagggatt ctgatgatct cgtcacccaa cagaccggtc tattcggaag aaagtgggca
     4681 gcacaacgaa ttccacgtca aggagctgga tttttccgag ttcgacagcc tgttacgcag
     4741 caaattttca cgcattcgat actttggtca gcgtttcatg atgggctcag ttcttcaacc
     4801 gcttgagggg ggaggcaagc attttgacgc gctatgcgaa cataagggtg ggatcaggaa
     4861 cagtattacg cccctgcagt atccggtcta tttcattgca gtctgtggtg acgaggcatc
     4921 cgctttcccg gaagtacgtt catccttcct gcttcccagg gaactcgatc tcctgcggca
     4981 gtatcagggt tacgcgaaat gggccgtgcg agtcgatgca gaactcgcta cttgccgaga
     5041 gctgtatagt aatcttgacg aagaacatca tcgcgtcgcc aattgggcga agagcctgta
     5101 tcaaagatgt gagcttctgg agcgcgaagt ggccgagaca gaggcgaagc tcagcgcgac
     5161 ccaggcgaaa ctcgatcaat cgattcgttc cgggtccagt agcgttgcca gactctcacc
     5221 attcgctgca ggtcttgccc gtggacaatg gactacggcc atgaaaccgc tgcgcacttg
     5281 gctgcgcacc tggctgcgca cctgggtgcg ttcccaaggg aagaggctat accaccaact
     5341 gccgctatcg caaacattca agctcaagct tgttggcggg atgtacaagt tcgccggtcc
     5401 tctttttgca ggtatgagcc attacgaaac gtggcaacac cagcggaatg gagtacccct
     5461 ccccatggca atgcaaggtg ccgtgaagct ggatgagatt gattccgtac ttcaggagct
     5521 aagcttcgaa ctgccagagc atccgctggt ttcgatcgtc attccagctt atggcaatct
     5581 gcccgttacc cttggctgtc ttcgctccat cgcgaggaat cttccggctg tcccggttga
     5641 agtcattctc atggaggact gttcgggaga tccggaaatc aatcgtttat cggaagtact
     5701 agggctgcgc tacctgcgga atccacagaa ccttggattc ttgcgctccg ccaaccgggc
     5761 tgccgatttc gcccaaggaa actatttcta tctcctcaat agcgataccg aggtcacaga
     5821 aggctggctg gatgcgatgc ttgccgtatt taaatcccac cctgactgtg gattggtagg
     5881 ttccaaactg gtatatccgg acgggcgctt gcaggaagct ggtggcatcg tctggaacga
     5941 cgcatctgcc tggaattacg gcaggttcca ggatccgtcg ctaccagaat tcaattacct
     6001 gcgcgaaacg gactactgtt ccggcgcctc aatcatgatt ccgcgggaac tgtacaagag
     6061 tctcggcgga ttcgacgaac gctatgtccc agcctatttc gaggataccg acctggcttt
     6121 tgcggttcgt tctgctggaa gaaaggtgta ttaccaacct gcttccgtcg tcatccacta
     6181 cgagggtgtt tcacatggca cggatactgg tgaaggtgtc aagtcctatc aggtgcaaaa
     6241 tcagcaaaaa ttcagggaga aatggcgaaa cgtacttgaa agagaccatt tccaaaatgc
     6301 gcatcactta tttcaggcac gtgaccgttc ccgctccaaa aaagcggttc tggtgattga
     6361 ccattatgta ccgcgaccgg atagggatgc cggttcccga tccatgctgg cgatcatgca
     6421 ggaattgctc gccatgggtt tcaatgtcaa gttctggcct cacaaccatt ggcaggatcc
     6481 tgtctatacc ccgcgcttgc aagccctggg tatcgaggtt gaatatggtt cccgttactg
     6541 ggggcgtttt gcgacctgga tcgaggagaa tggtaaatat cttgattatg ttctgctctc
     6601 gcgtccagac gtcgctgcgg atttcgtcca ggctttgagg aaatatacaa aattaccact
     6661 catttactat gggcacgata tacatcatct ccgattgaag gagcaattga aactggagcc
     6721 ggaaaatcat accgtccgca atgagatgaa gcgcatcgaa aagatcgaaa aacaacactg
     6781 gcgacaaatg gatgccattt attatccatc caacctggaa accgaggaag ttgccaaatt
     6841 cctcaagtct gaaggactgg aagataaatc ggtaacgctg ccggtttatg cgttcgactc
     6901 attttcatat aatgcggcag tcaatctggc cgatcgccac gatatccttt tcgtggcagg
     6961 attcggtcat ccgcctaatg ctactgccgc ccgctggttt gtgcacgaag tcttgccgct
     7021 ggtacgggaa agctttcccg atgtgcggct cacgctggta ggttccaatc caacaccaga
     7081 ggtgcttgct ctggcttgcg cggatatcgc tgtaactggc ttcgttactg acgagcaact
     7141 tgctgaatat tatcttaatg ctcgagttgc ggttgcccct ctgctgtttg gcgccggtat
     7201 gaaaggcaag gtggttgaag cgatgcgctt cggtattccg atggtgacca cgccgatcgg
     7261 ggcgcagggg ttggtggagg caggaagcgc tctgggggtt ttcgaagatc cctcggctta
     7321 tgccaaagat ataactcgct tgctgtccga tgacgaggag tggcgaatgc gatcagcggc
     7381 cgaacttgct tatgtccagg agcacttctc tttggctgcg atgcgttttt cattggagag
     7441 atggttcgtt ccgtaaggct gccgagca
//
LOCUS       DAOQJF010000150         7401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5508_length_7401_cov_5.029948, whole genome shotgun sequence.
ACCESSION   DAOQJF010000150 DAOQJF010000000
VERSION     DAOQJF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5508_length_7401_cov_5.029948"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLF85_09090"
     CDS             <1..630
                     /locus_tag="PLF85_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNE19862.1"
                     /translation="LYTFGARFHNRTEAGNKTASDQLIDYSIDVAYQGGQKGGQEIQA
                     YGGVAFIGVNFNPGLKMRIGGQGAYASGDDDATDGKYQTFDPMYPTPHYQFGQADMTS
                     WRNLVGGGINYTIWFTQEFNLKLDYWYAMRQNGNDSWYQISGAAFNSTGLGTEKHLYQ
                     EFGLIANYQARSYLAFQAGYAYAMRGKAMELANKSGDYQFGYLMSTISF"
     gene            complement(661..837)
                     /locus_tag="PLF85_09095"
     CDS             complement(661..837)
                     /locus_tag="PLF85_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19863.1"
                     /translation="MEKLTLWLLVLLLAVFAACKNSKECLSHCERSYAACVERTQDAE
                     KCLFFRQDCEGRCE"
     gene            979..3012
                     /locus_tag="PLF85_09100"
     CDS             979..3012
                     /locus_tag="PLF85_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE19864.1"
                     /translation="MAYRSALLLLGFAGLTFAQKTEKKTENSSIATPKESPAQTAGDF
                     SAEEEKPLQLRARRPVVLTASRNNERPEDEVINVETISGHYLANSGSSNIAEALSYQP
                     GLYVSYQGSRGDTVQMGGLPPEYSLVLINGRRVIGKNEEAINLSRLNLTDVDRIEVMK
                     GAGSAIYGSEAVGGVVNIITRPVSAPMEYKVLAGGGELGKFNLDAMAGSKLGIFSNKT
                     DVSFRQLEQYSISSSDPALKGRGFGNWQISDQLQFDVAKNWQVGVDVNAVQRRENGVT
                     RTTAYIDERKESQLTSGTLFSKSRLTDAQQLNVDLNYSYFFDRYVQDYQNPATRDSTT
                     DTKERFSSGLIHYNYSWMSNQTLTLGTENIWEQMESDRIATTTPGAAGVKERMRHAGY
                     AENKWIISRDLNLILQPAVRYDYSSTHRDAVSPRVGLRVDPVRDVTLKAGYGWGFRAP
                     SLKEMYYDFVSGANQRVLGNPDLKPEVSQSYNAGLAWTIVPGIIVSGNGYYHLIRDKI
                     ARVLDHTVGVTQFYLHTNADYAESKSGELGGEFHFLRYFSVRLGYTYTEATAQFTTTD
                     AATKVTSSMVRPLDGQAKHTGSATFMMEQRRWGLNLSVSVFGVGERPFYNRATRETEY
                     RSPYTDLQFRITKAVGIGTSVFISGENLTNDYNDTNLIRPPRSFWGGIIVQNF"
     gene            3070..5028
                     /locus_tag="PLF85_09105"
     CDS             3070..5028
                     /locus_tag="PLF85_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNE19865.1"
                     /translation="MRTKHIGLLLVLPALLLTQNCKEKQAVAQFTGPGKSLFEQKGCL
                     GCHGFGGGDKPTGPDLLGVTQRRGKEWLTRWIKDPAAMLKSDKDAQALLKKFNNVPMP
                     TLGLSDKESSDIVEYLAWMDSTGGGTKTAFVPLTDAEYEKGKEIFFNRCSGCHGAKRW
                     GATGPSLLPDSHIVAAKEVQGGGTKSKGTEALEAILWNGTPAGMPPWGKEGILSKKEV
                     NLMARFVQMTPPSIPPLDLNEMRNRWKLHVPVADRPKSDETNGRFKNYFGVILRDAGK
                     VAILDGDTKEKVAIIDTGFAVHILRSSHSGRYFYSIGRDGKVTLIDLWYKTPKMVAEG
                     RTCWDARSIDGSKAHGFEDKYAIIGCYTPNQYAIMDGQTLEPISNTSVEGVKDFATGN
                     ALPEVRVASIVASEKEPFWVINLKEAGWVYLVDYSDPKNPKETKLKADNFLHDGGWVR
                     LPGSDELRYFLVAANGVNRVCVVDVKLKKVQRPCIQTDKVPHPGRGANFVHPKYGPVW
                     ATPHIGAATISLIGVDPGKHPQYAWKEVERIKIKSAGSLFVKSHPKSNNLWFDMPLSS
                     QEGVNGEVGVYNIKTGEIKYLKASPKRITHMEYNAQGTEVWVSGWLEGTILVYDDATT
                     NLIKTVKEGWVQTPTGKFNVTNTSKDIY"
     gene            5185..5385
                     /locus_tag="PLF85_09110"
     CDS             5185..5385
                     /locus_tag="PLF85_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19866.1"
                     /translation="MDQNETQKRTLGQRAFDNIWFLLIIGMAFPGIFYLGWGMLEIFV
                     FNNTQLKDYLAQSNQQYLLPGK"
     gene            5395..5973
                     /locus_tag="PLF85_09115"
     CDS             5395..5973
                     /locus_tag="PLF85_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19867.1"
                     /translation="MPLETPKKNWWEPFNREERIWLYISIAWGIVMFIMMPLGHFWNQ
                     NISSETYKTNPEEYGQVAEKFIAKYQRKDAQGQPVTHNGKPGGIPVVDAPTKETGDTF
                     LVAQAWQFRPALVLKKNKTYRIHMSSLDYQHGFSLQPQNLNLQILPDYSFVITLSPNE
                     TGKFSLVCNEYCMFAGEKFGHDTMVGQVIVED"
     gene            5975..>7401
                     /locus_tag="PLF85_09120"
     CDS             5975..>7401
                     /locus_tag="PLF85_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE19868.1"
                     /translation="MSIADITNQFRTCPTTGLKVHLPAQRLIWINAVSAVVFLLVGGV
                     LALLIALTRWPAVHLLPQDLFYRFVTAHGITMLVFWIVFFEMAGVIFAAQTILGSRYP
                     GIKLGWFNYFLMITGAIMVEVVVFMGKADVMFTAYPPLKAHPAFYLGIILFAVGALIN
                     CFQFLYALVIAKREGWHKGSLPLFTFGILVAVIIAIFTLVSGAAAFIPAFLWSIGIID
                     RVDPEYFRISFWGFGHSAQQLNLAAMVSIWYLLANLTTGAKPVNQKMCRWAFVLYLLG
                     INLGSIHHNLVDPGLSATYRIFNTSYLFYAATIGSMIHAFSIPAAVETAQRAKGYTRG
                     LFQWLTNAPWGQPGFSGMALSMVIFGFFGGVTGIILSVEQLNMLSHNNLRVPGHFHMT
                     VVGGTTLAFMALTYYVIPLVLQRKLIGEKIAKYQPYVFAFGLMMFATGMHFAGVMGAP
                     RRHWDVTFQNAVIQVPFDPAMYTMLA"
BASE COUNT         1741 a         2151 c         1973 g         1536 t
ORIGIN      
        1 ctctatacct ttggtgcccg ttttcacaac cgcaccgaag cgggtaacaa aaccgcgagc
       61 gatcagctga tcgactactc gatcgacgtg gcctaccagg gtgggcaaaa aggtggccag
      121 gaaattcagg cctatggcgg tgtcgcgttt atcggcgtta actttaatcc cggtctgaaa
      181 atgcgcatcg gcggtcaggg cgcttatgcc agcggcgacg acgacgcaac cgacggcaag
      241 taccaaacct tcgacccgat gtacccgacc ccgcactacc agttcggcca ggctgacatg
      301 accagctggc gcaacctggt cggcggtggc atcaactata ccatctggtt cacgcaggag
      361 ttcaacctca agctcgacta ctggtacgcc atgcggcaga acggcaacga cagctggtac
      421 cagatcagcg gcgctgcgtt caattcgacg gggctcggca ccgaaaaaca cctgtaccag
      481 gaattcgggc tcatagcaaa ctatcaggcc cgcagctacc tggcatttca agccggttac
      541 gcgtacgcga tgcgcggcaa ggcgatggaa ctcgcgaaca agtcgggcga ttaccagttt
      601 ggttacctga tgtcgacgat cagtttctga tgaccgaacg ctgctcgtaa gggcagcgct
      661 ttattcacac cggccttcgc aatcctgcct gaagaacagg cacttctccg catcctgcgt
      721 gcgttcgacg caggcagcat aactgcgctc gcaatgcgaa aggcactctt tactgttctt
      781 gcaggctgca aatacagcga gcagcagcac cagtaaccaa agggttaatt tctccatggc
      841 caaaagtgcc atctgcaccg gcgcaagcaa gccttaaaaa acgattaacc gatccggaat
      901 tgcatttttt tgctctttta ttgatttaag tcaaggtaat ttgtctgcac ctgtgcttag
      961 gtcacggcag agatttctat ggcttaccgc tcggcgctgc tacttctggg tttcgcaggg
     1021 cttacatttg cccaaaaaac agaaaaaaag accgaaaact catcaatagc gacgccaaaa
     1081 gagagccctg cacaaactgc aggcgatttt tccgctgaag aagaaaagcc cctgcagctg
     1141 cgcgcccgcc gcccggttgt gctgaccgca tcgcgcaata acgaacgccc cgaggacgag
     1201 gtcatcaacg tcgagacaat ttcagggcac tatctcgcca actcaggttc aagcaacatc
     1261 gccgaagcac tgtcttacca gccggggctc tatgtttcgt accagggcag ccgcggtgac
     1321 actgtgcaga tgggcggact gccgcccgaa tacagcctcg tgctgattaa cggccggcgc
     1381 gtgatcggca agaacgaaga ggcgatcaac ctcagccgcc tcaacctcac cgatgtcgac
     1441 cgcatcgaag tcatgaaagg cgcgggttcg gccatctatg gctctgaagc agtcggcggc
     1501 gtggtgaata tcatcacgcg tcccgtcagc gcccccatgg agtacaaggt tctcgccggc
     1561 ggcggtgaac tgggtaagtt caacctcgat gcgatggcgg gctcaaagct cggcatcttc
     1621 agcaacaaga ccgacgtgtc tttccgccag ctcgagcagt acagtatctc gtcttctgat
     1681 cccgcgctca agggtcgcgg tttcggtaac tggcagatct ccgaccaact gcaattcgac
     1741 gtggcaaaaa actggcaggt tggtgtcgat gtaaatgccg tgcagcggcg tgaaaacggc
     1801 gtcacgcgca cgacggccta catcgacgag agaaaagaaa gccagctcac gtcgggcaca
     1861 ctgttctcga agagccgcct gacagatgcg cagcagctta acgtcgatct aaactactcg
     1921 tacttctttg accgctacgt gcaggactac cagaaccccg cgacacgcga cagcacaacc
     1981 gataccaaag agcggttcag cagcggactg atacactaca actacagctg gatgtcgaac
     2041 cagacactga cgctcggtac cgaaaacatc tgggaacaga tggaaagcga ccgcatcgca
     2101 accacgaccc ctggcgccgc aggcgtaaag gagcgcatgc gccacgcggg ttatgccgaa
     2161 aacaagtgga ttatttcgcg tgacctcaac ctgattctgc agcccgctgt gcgttacgac
     2221 tacagctcta ctcaccgcga tgcagtctca ccgcgcgtcg gtctcagggt cgacccagtg
     2281 cgcgatgtca cactcaaggc aggctatggc tggggttttc gtgcgccgag cctgaaagag
     2341 atgtactacg atttcgtctc cggtgcaaac cagcgcgtgc tgggtaaccc cgacctgaaa
     2401 cctgaagtct cgcagtcgta taacgccggc ctcgcctgga cgatcgtacc gggcatcatc
     2461 gtctcgggca acggttacta ccacctgatt cgcgacaaga tcgcgcgcgt tctggaccat
     2521 acggtaggcg tgacacagtt ttacctgcac accaacgccg attacgccga gagcaaaagc
     2581 ggcgagctcg gtggggaatt ccacttcttg cgctattttt ccgtgcggct gggttatacc
     2641 tataccgaag ccactgcaca gttcacaacc accgacgccg ctacaaaagt caccagctcg
     2701 atggtcagac cgctcgacgg gcaggcaaaa cacactggct cggcgacctt catgatggag
     2761 cagcgccgct gggggctaaa cctctcggtc agcgtgtttg gcgtcggcga gcgcccgttc
     2821 tataaccgtg caacgcgcga aactgaatac cgcagcccct acactgacct gcagttccgc
     2881 atcacgaagg ctgtcggcat cggcacttcc gtttttatca gcggcgaaaa cctgaccaac
     2941 gattacaacg acaccaacct catcagaccg ccgcgatcat tttggggagg catcatcgtt
     3001 cagaatttct gatggagcac cccggctgcg gcaacgcccg tttccgggga tattaaaaag
     3061 gagacaaaga tgagaacaaa gcacattggg ttgctgctgg ttcttccggc gcttctgctg
     3121 acgcagaact gcaaggaaaa gcaggccgtc gcccagttca cgggccccgg aaagagcctc
     3181 ttcgaacaga agggctgtct tgggtgccat ggtttcggcg gcggtgataa gcccaccggc
     3241 ccagacctgc tcggcgtcac acaacgccgc gggaaagaat ggctgaccag gtggatcaag
     3301 gacccggcgg cgatgctgaa atccgacaag gatgcgcagg cattgctgaa gaagttcaac
     3361 aatgtcccaa tgccgacgct cggcctttct gacaaagaaa gctccgacat tgtcgaatac
     3421 ctcgcgtgga tggattcgac aggtggcggc accaagactg cttttgttcc gttgaccgat
     3481 gccgagtacg agaagggcaa agaaatcttc tttaaccgct gctccggctg ccacggcgca
     3541 aaacgctggg gtgcaaccgg cccatcgctg ctgcccgatt cacacatcgt cgccgcaaaa
     3601 gaagtccagg gcggtggcac caagtcaaag ggtacagaag ctctcgaagc gattctctgg
     3661 aacggaactc ctgccggcat gccgccctgg ggtaaagaag gcattctttc caaaaaagaa
     3721 gttaacctga tggcgcgctt cgtgcaaatg actcctcctt cgatacctcc gcttgacctg
     3781 aacgagatgc ggaaccgctg gaaactgcac gtgccggtgg ctgatcgtcc aaaatctgac
     3841 gaaaccaacg gccgtttcaa gaactacttc ggcgtgattc tgcgcgacgc aggcaaggtg
     3901 gcgattctcg acggtgacac gaaagaaaag gtcgccatca tcgatacggg atttgccgtg
     3961 cacattctgc gctcctcgca ctcgggccgc tacttctact cgattggccg cgacggtaag
     4021 gtcacgctga tcgacctgtg gtacaaaaca ccgaagatgg tggcagaagg ccgcacctgc
     4081 tgggatgccc gctcgatcga cggctctaag gcgcatggct ttgaagacaa gtatgcgatc
     4141 atcggctgct acaccccgaa ccagtacgcg atcatggacg gccagacgct tgaaccgatt
     4201 tccaacacga gcgttgaagg cgtaaaagac ttcgccaccg gcaatgcgct gcccgaagtg
     4261 cgcgtcgctt cgatcgtcgc atctgagaaa gaaccgttct gggtgatcaa cctgaaagaa
     4321 gccggatggg tttatctcgt cgattacagc gatccgaaaa accccaaaga gaccaaactc
     4381 aaggccgaca actttctgca cgacggtggc tgggtacgcc tgcccggttc tgacgagctg
     4441 cgctactttt tggtggcggc aaacggcgtg aaccgcgtct gcgtcgtcga cgtgaaactg
     4501 aaaaaagtcc agcgtccctg catacagacc gacaaggtgc cacaccccgg ccgcggagcg
     4561 aatttcgttc acccgaaata tggccctgtc tgggcgacac cgcacattgg tgctgcgaca
     4621 atttcgctga tcggcgtaga cccgggcaaa cacccacaat acgcctggaa agaagtggag
     4681 cgcatcaaga tcaagtcggc aggttcgctg ttcgtcaaat cacacccgaa gtcaaacaac
     4741 ctctggtttg atatgccgct ttcttcacag gaaggtgtca acggcgaagt cggcgtctac
     4801 aacatcaaga ccggcgaaat caaatatctc aaggcttcac ccaagcgtat tacccacatg
     4861 gagtacaacg cacagggcac cgaagtctgg gtgtctggct ggcttgaggg caccattctg
     4921 gtttacgacg atgcaactac caacttgatc aagacggtca aagaaggctg ggtgcagact
     4981 ccaacaggaa agttcaacgt gaccaatacc agtaaagata tctattagtg atgcaaagga
     5041 ggtgcatcga tcgcacctcc ttctcaaaaa aaaaccggga tattgatcta gatcaaggaa
     5101 attttcctgt tcttatgtta aaaacccgtc actgtcagaa gtccgcccaa acggcttgac
     5161 tggcatactg cctgaggagg caaaatggac caaaacgaga cacaaaaacg cacgctgggc
     5221 cagagagcat ttgataacat ctggttcttg ctgattatcg gcatggcatt tccgggtatt
     5281 ttctacctcg gatggggcat gctcgaaatt ttcgtattca ataacaccca actgaaggac
     5341 tacctggcgc agagtaacca gcagtacctt ctgccgggca aataggagct agcaatgcct
     5401 ttagaaacgc ccaaaaagaa ctggtgggag cccttcaacc gcgaagagcg catctggctc
     5461 tatatctcga ttgcgtgggg catcgtcatg ttcatcatga tgccgctcgg tcacttctgg
     5521 aaccagaata tctcatcaga aacctacaaa accaaccctg aagagtatgg ccaggtcgcc
     5581 gagaagttca tcgccaaata ccagcgcaaa gatgcgcagg ggcagccggt gacacacaac
     5641 ggcaaacccg gcggtattcc cgtggtcgac gcaccgacaa aagaaactgg cgatacgttt
     5701 ctcgtagccc aggcctggca gtttcgcccg gcgctcgtgc taaagaagaa caagacgtac
     5761 cgcattcaca tgtcgtcgct cgactaccag cacggttttt cgctgcagcc gcagaacctg
     5821 aacctgcaga ttctgcccga ctacagcttt gtcatcaccc tgagtccgaa tgaaacgggc
     5881 aaattctcgc tggtgtgcaa tgagtattgc atgttcgcgg gcgaaaagtt cggccatgac
     5941 accatggttg gccaggtgat agtggaggat taacatgagt atagctgata ttaccaacca
     6001 gttcagaacc tgcccgacga ccggactcaa agtccatctg ccggcgcagc gcctcatctg
     6061 gatcaacgcc gtttcggccg tggtgtttct gctcgtcggg ggagttctcg ctcttctgat
     6121 cgcgctgacg cgctggcctg cagtacacct tctgccgcaa gatctcttct accgcttcgt
     6181 gaccgcgcac ggcattacca tgctcgtgtt ctggattgtc ttcttcgaga tggccggtgt
     6241 catctttgca gcgcagacga ttctcggttc gcgttacccc ggcataaagc tcgggtggtt
     6301 taactacttt ctgatgatta ccggcgcgat catggtcgaa gtggtcgtgt tcatgggtaa
     6361 agcagacgtg atgttcacgg cgtatccgcc gctgaaagcc cacccggcgt tttatctcgg
     6421 tatcattctc tttgcagtcg gcgctctgat caactgcttc cagttcttgt atgcgctcgt
     6481 cattgccaaa agagaaggat ggcataaagg cagcctgccg ctcttcacgt tcggtattct
     6541 cgtcgcggtt atcatcgcga tctttaccct tgtctcaggc gcagccgcgt ttatcccggc
     6601 attcctctgg tcgatcggca ttatcgatcg tgtcgacccc gaatacttcc gcatctcgtt
     6661 ctggggcttc ggtcactcgg cacagcagct gaacctcgcg gcgatggtat cgatctggta
     6721 cctgctcgct aacctgacaa cgggcgctaa acctgttaac cagaagatgt gccgctgggc
     6781 attcgtgctc tacctgctcg gtattaacct cggttcgatt caccacaacc tcgtcgaccc
     6841 gggtctctcg gcaacctacc gtatcttcaa caccagctac ttgttctacg ccgcgaccat
     6901 cggctcgatg atccacgcgt tctcgattcc ggcagcggtt gaaacggcac agcgcgccaa
     6961 gggttatacc cgcgggctct ttcagtggct gaccaacgcc ccgtggggcc aacccggttt
     7021 ctcgggcatg gcgctctcga tggtgatctt cggcttcttc ggtggcgtga ccggtatcat
     7081 cctgtcggtt gagcagctga acatgctgtc gcacaacaac ctgcgtgttc cgggccactt
     7141 tcacatgaca gtagtgggag gtaccactct cgcgtttatg gcgctgacct actatgttat
     7201 tccgctcgtg ctacaacgta agctgattgg cgaaaaaatc gccaaatacc agccatatgt
     7261 ctttgccttc ggcctgatga tgtttgccac cggtatgcac tttgccggtg tcatgggtgc
     7321 tccccgccgc cactgggacg tcacgttcca gaacgcggtg atccaggtgc cctttgaccc
     7381 ggcgatgtac acgatgctgg c
//
LOCUS       DAOQJF010000151         7299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5629_length_7299_cov_4.892877, whole genome shotgun sequence.
ACCESSION   DAOQJF010000151 DAOQJF010000000
VERSION     DAOQJF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5629_length_7299_cov_4.892877"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2872
                     /locus_tag="PLF85_09125"
     CDS             <1..2872
                     /locus_tag="PLF85_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE19869.1"
                     /translation="PADARLELGPDATGLGWVYQYVLRDKTGKNDLERLRSLQDFTIK
                     YQLLSVQGVAEVASVGGFQKQYQVTLDPVRLRASGISLNQVIDKVRGSNSQAGARLLE
                     IGGAEYMVRSQGYLKNKADMEQIGVGTDEAGNAVTLRSIATVTEAPALRRGVTDFMGE
                     GDAVSGVVVMRQGENAYEVISRVKHKVEEMKKFLPEGIELLPVYDRSELIEHSISNLK
                     IKLVEEMLIVALVILIFLGHFPSAIVPILTIPIAVLVSFIPLNLFGVSSNIMSLAGIA
                     ISIGVLVDGAIVEVENAYKKLEEWQRNGMKEDYHKVRLEALLEVGPSVFFSLLVVAVA
                     FLPIFTLVDQEGRLFKPLAYSKNLAMAVAAFMAITVDPAMRMLFTRVPKPHPASGTPP
                     LDQGRGWGRGFVPKALRGMGSRITHNLLVGKYHSEENHPISRRLIALYGPVCRYTLAY
                     PKRIIGGAVVLMLSMIPLYLHLGHEFFPALYEESLLYMPVTAPGIAPTEAARLLKLTD
                     QIIASHPEVERVFGKAGRADSATDPAPLSMFETNIILKPRSQWTVKTKEELIAKLDAS
                     LRLPGLTNAFTMPIRARVDMLSTGMRTPLGLKIQAREVKDIDVVAVKAEQLLKALPGI
                     RSVVAERTSTGYFVDIVPRRLDIARYGLSIDDVQLFVRTALGGEEITQTIEGVHRYGV
                     IVRYGADWRNSVEKLKRAPLGIQGGGQVPLSAIADVSLKTEPGMIRNENGFLTGYVFI
                     DTSSADIGGLAARIETELEKIKPLPEGVAIALSGQYENILRVRDRLLVVVPITIMLII
                     FLLYANTRSWTKTGIVMLAVPFSMLGALLLLTAMGIQISIAVWVGLIALMGLDAETGV
                     FMLMYLDLEYEARKKKIHNERDLKEAVYAGAVHRLRPKMMTVLAAFCGLLPIMFSSAT
                     GSDVMKAIAAPLVGGLFTSFLLELVIYPPVYFLWKRRKLVVA"
     gene            2932..3180
                     /locus_tag="PLF85_09130"
     CDS             2932..3180
                     /locus_tag="PLF85_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNE19870.1"
                     /translation="MEAVSYSDLRKNLKKYMDDVYSGHYPLIITRKNDENVVMLSLDE
                     FNALQETHYLLATEANTKHLKKSIAQHKSGKLRQVKIA"
     gene            3180..3437
                     /locus_tag="PLF85_09135"
     CDS             3180..3437
                     /locus_tag="PLF85_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNE19871.1"
                     /translation="MNVVFTDAAWSDYLYWQAEDRKTLKKINDLIKDIKRSPYEGLGK
                     PEPLRHQLQGYWSRRIDIEHRLVYKVEDRRKLVTIACRYHY"
     gene            3445..3939
                     /locus_tag="PLF85_09140"
     CDS             3445..3939
                     /locus_tag="PLF85_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19872.1"
                     /translation="MRPARCIVLSFFCIGSIFAQRSGLTPQTDNAIEFEVARLAEHLT
                     SEGFSCADRVLAGQAESIETQLTTEPMMAYILAVVTGRGGSAVPVQSFTVVNQGNKSF
                     EIPFENTRFGGHAAIAVRLPGDKILKVRLTQKATYRLLICKIETSVYRMKQFKPALAS
                     DGKL"
     gene            complement(3940..5562)
                     /locus_tag="PLF85_09145"
     CDS             complement(3940..5562)
                     /locus_tag="PLF85_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19873.1"
                     /translation="MQYLAGVALLVGALALPTNLAAVMNAEWQQGQDLVNAQNWVGAI
                     EYGESLSQKYPRHHYGQYLIYIGTGNLANTNPAYWETSAKAALKAASLEPARAPAHRA
                     HAVWCLWNLKDYAGIAAQASRLNAGAVQQIGEHNYLLQINYMTIAYVELGQLNTGARF
                     YLRLATPYADKPAIANTANAMSALVGKSAKNLPEPEKWFSFLVSVNKVAETNGYFLSP
                     LAMLALELGDEFYRRRDFARALHYFTTALDANRATLATRDGFLHDELRIRQVAATWRK
                     DNPQAKGRTERTAIMLIVPETRLNLPANAAEAERFKAGDSSAASFSANKKILSIAIPE
                     NQKNFQYFSDSVLALTGGEVEWKLKTIPLGGSRITAVEFHTDPRYNRWIAQAKPQFIE
                     PSLKGSVLKQVLAADATILMWPGTAKHGEIFVTNGGITEHPWVSGDETSHRALWVTEG
                     DKLLREGGSALFYHHEFFHLVEWGYYDRPFPKEDHQSQRRSEWPGNYTGTTEFDFYEQ
                     TYLKHVLPIDNLTRMEWKSGKTWLYKKKLEKR"
     gene            complement(5671..6558)
                     /locus_tag="PLF85_09150"
     CDS             complement(5671..6558)
                     /locus_tag="PLF85_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNE19874.1"
                     /translation="MARKRKAPLAPHQPVVAPVKAQPDMDKPQHSAWRRRVHEIVYES
                     DTRAGRLFDIILVGAILLSVLIIMLESVKSIEVNWHMELKIVEWFFTIIFTTEYALRL
                     ISVTKPIRYATSFFGVVDLIAILPTWLSIFLPGAQFLLSIRVLRLLRIFRILKLAHYL
                     SQAEILKQALAASQRKITVFLVTVTTIVIIVGSLIYVIEGEAHGFTSIPQSIYWAIVT
                     LTTVGYGDISPKTPLGQTLASLVMIMGYGIIAVPTGIVTAELTQIRHKEVSGQSCPAC
                     SLQGHDPDAQYCKFCGEKL"
     gene            complement(6622..7053)
                     /locus_tag="PLF85_09155"
     CDS             complement(6622..7053)
                     /locus_tag="PLF85_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19875.1"
                     /translation="MPGDLLAKAVAIAQRVHAQQVDKIGAPYILHVMRVMNRGQTLDE
                     KICGVLHDVVEDSPMTFDDLRKEGFPEHIIEALRCVTKTSEHEDYDAFIARIKTNPLA
                     VRVKLNDLEDNMDIRRLQAVGEKETARLNKYLKAYRDLTGI"
     gene            complement(7101..>7299)
                     /locus_tag="PLF85_09160"
     CDS             complement(7101..>7299)
                     /locus_tag="PLF85_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNE19876.1"
                     /translation="QATDRAHRIGQKKRVIVYKLISEGTVEEKVLTLQAQKRELFNDL
                     IDDSPAAAKRISRDDIGELLR"
BASE COUNT         1540 a         1979 c         2103 g         1677 t
ORIGIN      
        1 tcctgcagat gcgcggcttg agctggggcc cgatgcgacc ggactgggtt gggtgtacca
       61 gtacgtgctg cgcgacaaga cgggtaagaa cgatctcgag cgcctgcgtt cgctgcagga
      121 ctttaccatc aagtaccagc tgctttcggt gcagggtgtt gccgaggtcg cgagtgtcgg
      181 cggtttccag aaacaatacc aggtgacact cgaccccgta cggttgcgcg cttcgggaat
      241 ttccctcaac caggtgatcg acaaggtgcg cggcagcaac tcgcaggccg gggctcgcct
      301 gctcgaaatc ggcggcgccg agtacatggt gcgcagccag gggtacctga agaacaaagc
      361 cgacatggag cagatcggcg tcggcaccga cgaggcgggc aatgcggtca ccttgcgttc
      421 gattgcgacc gtgaccgagg cgcccgcact gcggcgcggc gtgactgact tcatgggcga
      481 aggcgacgcc gtttcgggtg tggtggtcat gcggcagggc gagaacgcct acgaagtcat
      541 tagccgggtg aaacataaag tcgaagagat gaagaagttt ttgcctgagg gtattgagct
      601 gctgcccgtc tacgaccgct cggagctgat tgagcacagc atctctaacc tgaagatcaa
      661 gctggtagaa gagatgctga tcgttgcgct cgtgatactc atctttctcg gtcactttcc
      721 ttcagcaatt gtgccgatcc tgacgatacc gattgccgtg ctggtttctt ttataccgct
      781 caacctgttt ggcgtgagtt cgaatatcat gtcgctcgca ggcatcgcga tatccatcgg
      841 cgtactggtc gatggcgcga ttgtcgaggt cgagaacgct tacaagaaac tcgaagagtg
      901 gcagcgcaat ggtatgaaag aagattacca caaggtgcgc ctcgaagccc tgctcgaagt
      961 tggcccttct gtctttttct cgctgctggt agtagcggtg gcgttcttgc caatctttac
     1021 cctggtcgac caggaagggc ggctcttcaa gccactggca tactcgaaga atctcgcgat
     1081 ggctgtcgcg gccttcatgg cgatcacggt cgaccccgcg atgcggatgc ttttcacccg
     1141 ggttcctaag ccccaccccg cctccggcac ccctcccctg gatcagggga ggggctgggg
     1201 gaggggtttt gtaccgaagg ccctgcgtgg aatggggtcg cgaatcacgc acaacctgct
     1261 cgtcggcaaa taccactcag aagaaaacca tcccatcagc cgtcgcctca ttgcgctcta
     1321 tgggccggtc tgccggtata cactcgcgta cccgaagcga atcattggcg gcgcagtggt
     1381 gctgatgctt tcgatgatcc cgctctacct gcaccttggg catgagtttt ttcctgcgct
     1441 gtatgaagaa tctctgctct acatgcctgt caccgcaccc ggcatagctc ccactgaagc
     1501 tgcgcgcctg ctgaagctga ccgaccagat cattgcctcg caccccgagg tggagcgtgt
     1561 gttcggcaag gcgggtcgtg ccgattcggc gactgaccct gcaccgctgt cgatgttcga
     1621 gacgaacatc atactcaaac cgcgttcgca atggacggta aaaaccaaag aggagcttat
     1681 tgcgaaactc gatgcctcgc tgcgattgcc gggtctgacc aacgcgttca cgatgccgat
     1741 ccgcgcgcgc gtcgatatgc tctctacagg tatgcgcaca ccactgggtc tcaagatcca
     1801 ggcgcgcgag gtgaaagata ttgatgtggt ggctgtcaag gctgaacagc tgctgaaggc
     1861 attgccgggc atacgcagcg tggtcgccga gcgcacatca accggatatt tcgtcgatat
     1921 cgtgccgcgc cgcctcgaca ttgcgcgtta cggcctgtcg atcgatgatg tgcagctgtt
     1981 tgtgcgcacc gcacttggcg gcgaagagat cacgcagaca attgaaggtg tgcaccgcta
     2041 tggtgtcatt gtgcgttatg gcgccgactg gcgtaactcg gtagaaaaac tgaagcgcgc
     2101 gccactgggt attcagggcg gggggcaggt gccgctttct gcgatcgccg acgtctcact
     2161 caagactgaa cccggcatga tacgcaacga gaacggtttt ctcacgggct atgtgttcat
     2221 cgataccagc agcgccgaca ttggcgggct tgccgcacgt attgaaaccg agctggaaaa
     2281 aattaaacca ttgcccgaag gtgtggcgat tgctctcagc ggccagtacg aaaatatcct
     2341 gcgggtacgc gaccggttgt tggtcgttgt gccgattacc atcatgctga tcatcttttt
     2401 gctctacgcc aatacccgct cctggaccaa aaccggcatt gtcatgctcg cggtgccgtt
     2461 ctcgatgctc ggcgcgcttc tgctgctcac cgcgatgggc atacagatct cgatagccgt
     2521 ctgggtggga ttaatcgctc tcatgggcct cgatgccgag actggagtct ttatgctcat
     2581 gtacctcgat ctcgagtatg aagcgagaaa gaaaaaaatc cacaacgagc gcgacctgaa
     2641 agaggcggtg tatgccgggg cggtgcaccg gctgcggccg aagatgatga ccgtgctcgc
     2701 agcgttctgc ggcctgttac cgatcatgtt ttcatcggcg actggttccg atgtgatgaa
     2761 ggctattgcg gccccgctgg tgggcgggct gttcacgagt ttcctgctcg aactcgtgat
     2821 ctatccgccc gtgtattttc tgtggaagag gcggaagttg gttgtagcgt gaggacattt
     2881 tcgctcaggt aaatagtgga ccgcttgcca tatgtacaat aaattgtaca tgtggaagcc
     2941 gtgtcttaca gcgacttgcg caagaatttg aaaaaataca tggacgacgt ctactcgggc
     3001 cattacccgc tgatcattac ccgcaagaac gacgaaaacg tggtgatgct gtcactcgat
     3061 gagttcaatg cgctgcaaga gacacactat cttctggcta ccgaagctaa taccaaacat
     3121 ctcaaaaaat ccattgcgca gcataaatcc ggcaaactca ggcaagtcaa gattgcctga
     3181 tgaatgtcgt ctttaccgat gcagcctggt cagattacct atactggcag gcagaagacc
     3241 gaaagacgct caaaaagatc aacgatctca taaaagatat taaaagatcc ccatacgaag
     3301 gcttgggtaa gcccgagccc ttgaggcacc aattgcaggg gtattggtcg agaagaattg
     3361 acatagagca ccgtttggtg tataaagtcg aagatcgcag gaaactcgtt accattgcct
     3421 gcaggtacca ttactgatct tatcgtgcgt ccagctcgct gcatagttct ttctttcttc
     3481 tgcatcggca gcatttttgc ccagcgcagt ggcctgacgc cgcaaactga taatgcgatc
     3541 gaattcgaag tcgcccgcct ggcggagcac ctcacctcag agggtttttc ctgcgcggac
     3601 cgtgtgctcg ccggccaggc tgaaagtatt gagacgcagc ttactaccga gccaatgatg
     3661 gcttatatac tggctgtcgt gaccggccgc ggcggcagcg ctgtcccggt gcagagtttc
     3721 accgtagtaa accaaggcaa caaatcgttt gagatcccct tcgagaatac ccgctttgga
     3781 gggcatgcgg caatcgccgt gcgtttgccg ggagacaaaa tactgaaggt tcgcctgacg
     3841 cagaaagcga cgtaccgcct gctcatctgc aagatcgaga cgtcggtcta ccgcatgaag
     3901 cagttcaagc ctgcgcttgc cagtgatggc aagctctgat cagcgttttt ccagcttttt
     3961 cttatagagc caggtcttgc cgctcttcca ctccatgcgt gtaaggttgt cgatgggtaa
     4021 aacatgcttc aggtacgtct gttcgtagaa gtcgaattct gtggttccgg tgtaattgcc
     4081 gggccattca ctgcgtcgct gtgactggtg gtcttctttt ggaaagggcc ggtcgtaata
     4141 gccccactcc accagatgaa aaaattcatg gtggtagaag agcgccgatc cgccttcgcg
     4201 caaaagttta tcgccttcag taacccagag tgcgcggtgg ctggtttcat cgccagaaac
     4261 ccacgggtgt tcggtgatgc cgccgttggt gacaaaaatt tcaccatgct tcgctgtgcc
     4321 aggccacatc aggattgttg cgtcggcggc gagcacctgt ttcagaactg aacccttgag
     4381 cgacggttca ataaactgcg gtttggcctg cgctatccag cggttgtacc gcgggtcggt
     4441 gtgaaactcc accgcagtga tgcgcgaacc accaaggggg attgtcttca gtttccattc
     4501 tacctcgccg cctgtcagcg cgagtacgct gtctgaaaag tactggaagt ttttctggtt
     4561 ctcgggaatc gcaatcgaaa gaattttctt gttagctgaa aaagaggcag ccgaagagtc
     4621 acccgctttg aagcgttctg cttcggcggc attcgccggc aggttgagcc gcgtctcggg
     4681 cacgatcagc atgatcgccg tgcgctcggt gcgacccttt gcctgcgggt tatcttttcg
     4741 ccaggtggct gcaacttgcc tgatacgcag ttcatcgtgc aaaaagccgt cgcgcgtcgc
     4801 gagggtagcg cggttggcat cgagggcagt cgtgaaatag tgcagtgcgc gcgcaaagtc
     4861 ccgtcggcgg taaaactcgt cgccgagctc gagtgcgagc atcgcgagcg ggctcaggaa
     4921 gtatccgttg gtctcagcca ccttgttgac gctgaccagg aagctgaacc acttctctgg
     4981 ctcgggcaga ttttttgccg atttgcccac cagcgcactc attgcgtttg cagtattggc
     5041 aattgcaggt ttatcggcat aaggtgtggc aagccgcagg tagaagcgcg cgccggtatt
     5101 gagttgaccg agctctacat aggcaatcgt catgtagttg atctgcagca ggtagttgtg
     5161 ctcgccgatc tgctgaaccg cgcctgcatt caggcgcgaa gcctgcgcgg cgatgcccgc
     5221 ataatctttc agattccaga ggcaccagac agcgtgtgca cggtgcgccg gagctcgtgc
     5281 gggttcaagg ctggccgctt tgagcgcagc cttggccgaa gtctcccagt aagctgggtt
     5341 cgtgttggca aggttgcctg taccaatata gatgaggtac tgcccgtaat gatggcgtgg
     5401 gtacttctgg ctcaggctct cgccgtattc gattgccccg acccagttct gcgcattgac
     5461 gagatcctgt ccctgctgcc actcagcgtt cataaccgct gcgagattcg ttggcagggc
     5521 taatgccccg accaaaaggg cgacgccggc taagtattgc atagttatta gacggttgtg
     5581 cagcataggc acctcaatgt cagcccgttg cagattgtaa atggaaagtt ctaccctgcg
     5641 aatctctact aagcagagat ttgcaaggtt ctataatttc tcgccgcaga atttgcagta
     5701 ctgcgcatcc gggtcatgcc cctgcaggct acaggccggg catgactgcc ccgagacctc
     5761 tttgtgccgt atctgtgtta gctccgcagt gacaataccc gtcggcacgg cgataatgcc
     5821 gtaacccatg atcatcacca gcgaagcgag ggtctgcccc agcggagttt tcggcgagat
     5881 gtcgccgtaa cccactgtgg tcagtgtcac gattgcccag tagatactct gcggtatact
     5941 cgtgaagcca tgtgcctcgc cttcgatgac atagatcagc gacccgacga tgatcacgat
     6001 ggtcgtaacg gtcacaagaa agactgtaat cttgcgctgg ctggccgcaa gagcctgttt
     6061 aagaatctct gcctgtgaaa ggtagtgcgc cagcttgagg atacgaaaga tgcgcaacag
     6121 gcgcagcacc cgtatagaga gaagaaactg tgcacccgga agaaaaatgc tcagccaggt
     6181 cggtaaaatg gcaatgagat cgacgacgcc gaaaaagctc gtggcgtacc gtattggctt
     6241 ggtgacagag ataagcctca aagcgtattc ggtggtaaaa attatcgtga aaaaccactc
     6301 gacgatcttt agctccatgt gccagttcac ctcgatgctc tttacgcttt cgagcatgat
     6361 gatcagcacg ctcagcaaga tcgcgccgac gagaatgatg tcaaacaggc gaccggcccg
     6421 cgtatctgac tcgtagacga tttcgtgcac gcggcgacgc cacgcggagt gctgtggctt
     6481 atccatatct ggttgcgctt ttactggagc gacaactggc tggtgcggcg cgagaggagc
     6541 ttttcgtttg cgggccatgg ggcgagcccg cgtggaagac ttagagcgca tgtaaagccg
     6601 atggccaccc tctcggggcg gttatatgcc agtcaggtcc cggtaggctt tgagatactt
     6661 gttaagccgc gcggtctctt tttcgcccac cgcttgcaac cggcggatat ccatgttgtc
     6721 ttcgagatca ttgagtttaa cccgcaccgc cagtggattg gttttgatgc gggcaataaa
     6781 agcatcgtag tcttcgtgct cggaagtttt ggtcacacag cgcagcgctt cgatgatgtg
     6841 ttcgggaaat ccttctttgc gcaggtcgtc gaaggtcatg ggcgaatctt cgaccacgtc
     6901 gtgtaatacc ccgcagatct tctcgtcgag cgtctgcccg cggttcatga cgcgcatgac
     6961 gtgcagtatg tacggggcgc cgatcttgtc gacctgctgc gcatgcacac gctgcgcgat
     7021 cgcgacggct ttcgccagta aatccccagg catggttgag aacagaaatt ccgggcattg
     7081 ccgtaaacca ttttgcctgc tcagcgcaac agttcgccga tgtcgtcgcg cgagatacgt
     7141 ttcgccgctg ccgggctgtc gtcgatcagg tcattaaaca gttcgcgttt ctgcgcctgc
     7201 agcgtgagta ccttttcttc taccgtacct tcgctgatca gcttgtagac aatcacgcgc
     7261 ttcttctggc cgatgcggtg cgcgcggtcg gtcgcctgg
//
LOCUS       DAOQJF010000152         7275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5649_length_7275_cov_4.255125, whole genome shotgun sequence.
ACCESSION   DAOQJF010000152 DAOQJF010000000
VERSION     DAOQJF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5649_length_7275_cov_4.255125"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLF85_09165"
     CDS             <1..199
                     /locus_tag="PLF85_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19877.1"
                     /translation="LIVSAGYEGHWWQANPYSSDKLVRTGFVADSGFTDQNYGGFEHR
                     TRTSWEAFATIAVELHFDGKS"
     gene            complement(206..847)
                     /locus_tag="PLF85_09170"
     CDS             complement(206..847)
                     /locus_tag="PLF85_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19878.1"
                     /translation="MRFPAPAAALALAAFTFQPASPEVFPANQPPVLASDSRLNPLPP
                     EVLAFPEHSENYREKIIAGDYEIWSEERLQRWSSAQGLSVTPDNTAAGLNYHRAAIVS
                     APGLTFRLRRPVTEKGSEFANGWTLNLDIAAIRARDGQSNLARYQNLLECEVLVDDVL
                     YKTIRQGSHASAVSPVKIPIPHIRSTDGVITVRLRMANHPRNFLFLYDAFLSR"
     gene            complement(930..2033)
                     /gene="ychF"
                     /locus_tag="PLF85_09175"
     CDS             complement(930..2033)
                     /gene="ychF"
                     /locus_tag="PLF85_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNE19879.1"
                     /translation="MPLQCGIVGLPNVGKSTIFNALTKSHSAEAQNYPFCTIDPNVGV
                     VAVPDPRYDFLVDIVKPKNSVPVAVEFVDIAGLVKGASQGEGLGNKFLDHIRKVNAIL
                     HVVRCFEDENITHVSGAVSPRSDVEIIELELILSDLDQVDKRLQNLSKKKKSGDKEAL
                     EQSAVLERVYDLLKDGKPASAAGLNEEEKELIKDLALLSLKPVLFIGNIDESLLAKPE
                     DSPQFRELVALAHERGAQAIPLSGKIEAELGQLSPEEEREFLKDLGLTEPGLNRVIRE
                     AYTLLGYITFFTAGEVEVRAWNIVRGATAPQAAGTIHSDIERGFIRAEVTPFAAIEEY
                     GTQKKAQEAGKMRLEGKEYIMQDGDVVYFRFNV"
     gene            2238..3254
                     /locus_tag="PLF85_09180"
     CDS             2238..3254
                     /locus_tag="PLF85_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19880.1"
                     /translation="MRPEKVFTLPVGEEKANFPRQGGVYHEIPGRVGVIQDWVAFSEP
                     SQRSVKLYRAGKPVFSLRGGEAGKAKGETKNLEALRIPGRIVMGGNDDFFVENYLPPE
                     GENKDATSRGGYKILQFDFKGKLLRSIGRKGLSELVFENILWFDTDTKGYLWVLYRYL
                     DELHLDRYEGNRLAFEYRQTDCEAMTLDKKQIDMLKDRNSIVHCEYIYPFYSGERIML
                     SSRIDRVDPESDKKQLTLAYRIVKVKGLSDSSAETVFSRLSNAEDHPYLPQGKDSVGI
                     WQNLEGGRMRIARYSLDGDLRNSIVIELSGRPSEWRSVWQNLSGEVYGIRLFADQFEV
                     HRFR"
     gene            3254..4255
                     /locus_tag="PLF85_09185"
     CDS             3254..4255
                     /locus_tag="PLF85_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19881.1"
                     /translation="MGVLRKEWPLLLSALVFLALMVADYFTGFMTGFTSLTSGRQSDL
                     FVSAGIVTVITLAFLEGILGIDNATVLAIQVRHLKPAEAHKALTWGIWGAFIFRFIFL
                     LAAGFILEQKWIMAIGGFYLINMAADFFLKNLPQLLTDMLVLGVLFCVFFVSRETIYL
                     AGIQLPVWTFFAALAAYSGYRYFAQQRRHAAHTDETEEGIHISRFRLARYPVLAAIIA
                     VEWTDILFSFDSIGAGVAMTRDFWVLFWGAFFGVTCLRLFAKSFIRLLERYPQLEGAA
                     MIAVLLVGAKMAFEAAQDTLKVRLWHVENWQTSLVIVLIFTGAFFVRAKTPHISEET"
     gene            4252..4701
                     /locus_tag="PLF85_09190"
     CDS             4252..4701
                     /locus_tag="PLF85_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNE19882.1"
                     /translation="MKILKSLQAALVFGLFAISSASGERADLHQRVTGLAQKYQLDAQ
                     ELYTAVDSVRNTGRLPAKWITKAEASRLGWHPGKPLGRIAPGKSIGGDRFGNREGLLP
                     VKTGRTWYEADLSYRGGKRNAKRLLYSSDRWIFVTADHYQTFERILP"
     gene            4698..4970
                     /locus_tag="PLF85_09195"
     CDS             4698..4970
                     /locus_tag="PLF85_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNE19883.1"
                     /translation="MTRILLNADRIFDQQSFFRELALQTQLPDYFGNNLDALFDFLTI
                     DLAGPVEIAWPRKNGVPEAIQNTLESVRQTLRDAALERADLYFTED"
     gene            complement(4957..5217)
                     /locus_tag="PLF85_09200"
     CDS             complement(4957..5217)
                     /locus_tag="PLF85_09200"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNE19884.1"
                     /translation="MTRLFGHVYLPMGQKIAQGIQKITELLIASPCTAVCSMDEKREL
                     CTGCYRTLDEIMQWSRFNGEKKHQVNTAAAERRAAAQKTNPR"
     gene            complement(5302..5772)
                     /locus_tag="PLF85_09205"
     CDS             complement(5302..5772)
                     /locus_tag="PLF85_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE19885.1"
                     /translation="MQVARNKVVTISYELSESGKGKLEDGTLDYLHGFKNLMPALEKF
                     LTDKKEGFSGKVEMGPRDAFGEFSQDMVMTIPKDQLEKPEEMQVGMQIELESEEGVIP
                     ALVTAVGADSITVDGNHPYAGKTLTFDIKVVAIRDASAEELAHGHAHGAHGHHH"
     gene            complement(5782..6504)
                     /locus_tag="PLF85_09210"
     CDS             complement(5782..6504)
                     /locus_tag="PLF85_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19886.1"
                     /translation="MKIKRSGKTKTPSRRAKKAVLRARKPVLPVKKPKKTVARAKKPA
                     KPDMAPSVDFVQPSAPATEALSPFAALVSEHTIANAWDILGDEEFVLDDEVWNTLYNR
                     IKLQLLQASYSFRVDTDSVSLSPWSVTPMPAFRGDNEPGFLKAGSFTVLKPELPGVIF
                     DLKISANDQSLLMDWSLRRKDSAASTGHMALYCDGELVEAVNLNQSRCQLELTRDDLG
                     DISFYFLDAETGKQTKILELSV"
     gene            complement(6501..>7275)
                     /locus_tag="PLF85_09215"
     CDS             complement(6501..>7275)
                     /locus_tag="PLF85_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19887.1"
                     /translation="LHLLIYNYAQLRHQQSTDVSADFYLHVAGKLDGILQRYDARRLP
                     FYQYMASSLNFEFSHFLRRRKMPRSQVELLSVEELAERRLELQYRENPTPDPLSPLLA
                     KMRPPTSAYARLALACPLTYGELRYLLQRGRQKDGSHWKMLRSYRAFLRYAEEKRVAF
                     IRERDRLLQILMRTEQHERTANTAALQALKKRRESARRKFFSMDTRIPIRIVAEVTGD
                     SIATAQRQLKAATETLKSVYARWKKQDLLEIQRKSQRRN"
BASE COUNT         1457 a         1969 c         2086 g         1763 t
ORIGIN      
        1 gctcattgtc agcgcgggat acgaaggcca ttggtggcag gcaaaccctt acagttcaga
       61 taagttggtc cgaacaggat tcgttgccga cagcggcttt accgaccaga attatggcgg
      121 ctttgagcac cgcacgcgta cctcgtggga agcctttgct accatcgccg ttgaactgca
      181 tttcgacggt aaatcgtgac ttgtttcaac ggctgagaaa cgcgtcgtag agaaagagaa
      241 agttccgcgg gtggtttgcc atgcgcagcc gaacggtgat cactccgtcg gtactgcgga
      301 tatgcggaat cggtatcttt accgggctca ctgcagatgc gtgtgaaccc tggcgtattg
      361 tcttgtagag cacatcgtcg acgagaactt cacattcgag cagattttgg taacgcgcga
      421 ggttcgactg gccgtcgcgg gccctgattg cagcgatatc gagattgagc gtccagccgt
      481 tggcaaattc gctgcccttt tctgtcacag gcctgcgcag gcgaaaggtc agccccggtg
      541 cgctcacgat cgcggcgcgg tggtaattca ggccggccgc agtattgtca ggcgtaaccg
      601 aaagaccctg cgcgctgctc cagcgctgca gcctctcttc tgaccagatc tcataatcgc
      661 cggcaataat cttttcccgg taattttcgc tgtgttccgg aaatgccagg acttcaggcg
      721 gcagcggatt cagccggctg tccgaagcga gcaccggcgg ctggtttgcc ggaaaaactt
      781 cggggctggc aggctgaaac gtgaaagctg ccagcgcgag ggctgcggca ggagcaggaa
      841 agcgcatacc ttattgtcgg ccagggaggg gcaggggtga atgcccgccc ggcaaaaagc
      901 gcccgccggg tacaccgctg ttgtgagaat cagacgttga agcggaagta gacgacgtcg
      961 ccatcctgca tgatgtactc tttgccctca aggcgcatct tgccggcttc ctgggctttt
     1021 ttctgggtgc cgtactcttc gatcgcggca aacggtgtca cctcggcgcg gataaatccc
     1081 cgctcgatat cgctgtggat ggtgcccgct gcctgcggag ccgttgcgcc gcggacgata
     1141 ttccaggcgc gcacctcgac ttcacctgca gtaaagaagg tgatataacc gagcagcgtg
     1201 tatgcctcac ggatgacgcg gttcaggccg ggttcggtta atccgaggtc cttgagaaac
     1261 tccctctctt cttctggcga aagctgcccg agttcggcct cgattttgcc agagagcgga
     1321 attgcctgtg cgccgcgttc gtgcgcgagt gctaccagtt cgcggaactg cggcgaatct
     1381 tctggctttg ccagcagcga ttcatcgata ttgccgataa acagtaccgg tttcaggctg
     1441 aggagtgcga ggtctttgat cagttctttt tcttcttcgt tcaggcccgc cgcgctcgct
     1501 ggtttgccat ctttcagcag gtcgtacacc cgttcgagca ctgcgctctg ttccagcgct
     1561 tctttgtcgc cgctcttctt ctttttgctc aggttctgca ggcgcttatc gacctggtcg
     1621 aggtccgaga gaatcagctc gagctcgatg atttcaacat cgcttcgcgg agagacagcg
     1681 cctgaaacat gcgtgatatt ttcgtcttca aagcaacgca ccacatggag aattgcattc
     1741 accttgcgga tatggtcgag aaacttgtta cccaggcctt cgccttgtga agcacccttt
     1801 accagcccgg caatatcgac aaactccacc gcaacgggaa cgctgttttt aggttttacg
     1861 atatctacca gaaaatcata acgcgggtcg ggtacggcaa cgacacccac gtttgggtca
     1921 atggtacaga acgggtagtt ctgcgcctct gcgctgtgtg atttggttag cgcgttgaag
     1981 atagtcgatt taccgacgtt gggcaggccc acgattccgc attgtaatgg catgaggcag
     2041 aaccggggat cgagacaggc cttcatgcac taattcagtg gcaataatac cgctgcgaag
     2101 cgacttgcgc ggcgacaaaa agttttgagt tcccggctga actcgacaat tggcaacggt
     2161 tagacgtgcg ggcagctggc tggctattct tatcggttct ggcattgaac ggctgcgcgc
     2221 gttttcgtac gagcaccttg cgtcccgaga aggtttttac ccttccggtg ggcgaagaaa
     2281 aagccaattt cccgcggcag ggcggcgtct accacgaaat tccggggcgg gtgggcgtga
     2341 tacaggattg ggtagccttc tcagaacctt cgcagaggtc ggtcaaattg taccgggccg
     2401 gaaaacccgt cttcagtctg cgcggtggcg aggcaggtaa agcgaagggc gagacgaaga
     2461 acctcgaagc gctgcgtatt cccggccgta ttgtcatggg cggtaacgat gacttttttg
     2521 tagagaacta cctgccaccc gagggtgaaa acaaagacgc cacaagccgc ggcggctaca
     2581 agattctcca gtttgacttc aagggtaaac tgctgcgttc cattggccgc aagggtcttt
     2641 cagaactggt atttgagaac atcctctggt ttgataccga tacgaaaggt tacctgtggg
     2701 tgctgtaccg ttacctcgac gagctccacc tcgaccgcta cgaaggcaac cggctggcgt
     2761 tcgagtacag gcaaacagac tgcgaggcga tgacgctcga caagaagcag atcgacatgc
     2821 tgaaagaccg caattcaatc gtgcactgcg agtacatcta tccgttttat tcaggcgagc
     2881 gcatcatgct ctcgtcgcgc atcgatcgcg tagatccgga atccgacaag aagcagctga
     2941 cattggcgta ccggatagtc aaggtcaaag gtcttagcga cagctcagcc gagacagtct
     3001 tttcccgcct cagtaacgcc gaagaccatc cctacctgcc gcaaggcaaa gacagtgtcg
     3061 gcatctggca gaacctggaa ggtgggcgca tgcgtattgc gcggtattct ctcgacggtg
     3121 acctgcgaaa ttccatcgtg atcgagctga gtggtcgccc gagcgaatgg cgttctgtct
     3181 ggcagaacct gtcgggcgag gtctacggca ttcgcctttt tgccgaccag tttgaggttc
     3241 accgctttcg ctaatgggtg tcctgcgcaa agaatggccg cttttgcttt cggccctggt
     3301 tttcctggcg ctgatggttg ccgattattt caccgggttc atgacggggt ttaccagcct
     3361 gacttctggc aggcagtctg atctttttgt ctcggcaggt attgttaccg tcatcactct
     3421 cgcctttctt gaaggtattc tgggtatcga taacgctact gtactggcaa tacaggtgcg
     3481 acacctcaaa cccgccgaag cccacaaggc gcttacctgg ggtatctggg gagctttcat
     3541 tttccggttc atttttctgc tggcagcagg cttcatcctg gagcagaagt ggattatggc
     3601 aatcggtggg ttttacctca tcaacatggc ggcagatttt ttcctcaaaa acctgccaca
     3661 gctgctgacc gacatgctgg tgctcggggt gttattttgt gttttcttcg tctcgcgcga
     3721 gactatttac ctggcaggca tccagcttcc tgtctggacg ttctttgccg cgctcgcggc
     3781 atattccggg taccgctact ttgcgcagca gagacgccat gctgcgcaca ccgacgagac
     3841 tgaagaaggc atccacattt cgcgtttcag gctggcacgt tacccagtgc tcgccgccat
     3901 tatcgccgtc gaatggaccg atatcctgtt ttcttttgat tcgatcggcg ccggtgtcgc
     3961 gatgacgcgc gacttctggg tactgttctg gggagcgttt ttcggcgtca cctgcctcag
     4021 gctgtttgcc aaatccttta ttcgcctgct agagcgttat ccacaactcg aaggagctgc
     4081 gatgatcgcg gtgctccttg tcggcgcgaa gatggctttt gaagccgcgc aggatacact
     4141 gaaggtcagg ctctggcatg tcgaaaactg gcagaccagt ctggtgattg ttttgatttt
     4201 caccggcgcg ttttttgtcc gcgcgaagac tccccacatc agcgaagaga catgaagatt
     4261 ctgaagtctc ttcaggcggc gctggtcttt gggctgttcg ccatcagctc tgccagcggc
     4321 gaaagggctg acctgcacca acgggttacc gggctggcgc agaaatacca gctcgacgcg
     4381 caggaactct acacagcggt tgactccgtc aggaacaccg gccgtctgcc ggcaaaatgg
     4441 attaccaagg ccgaagcatc gcggttgggg tggcatcccg gcaagccact ggggcgcatc
     4501 gcgccgggca agagtatcgg cggtgaccgt tttggtaacc gcgagggact tttgccagtc
     4561 aagactggca ggacatggta cgaagcagat ctttcgtacc gcggtggtaa acgaaacgcc
     4621 aagcgcctgc tgtattcgag cgacaggtgg atattcgtga cagcagacca ttaccagaca
     4681 ttcgagagga tactgccatg actcgcattc tgctcaacgc tgaccgtatc tttgaccagc
     4741 aatctttttt tcgcgagctc gccctacaaa cgcagctgcc agattatttc gggaataacc
     4801 tcgatgcgct ttttgatttc ctgacgatag atcttgccgg tcctgtcgaa atcgcctggc
     4861 cgcgcaagaa cggcgtacct gaagctatac agaataccct tgaatcggtg agacaaacgt
     4921 tgcgggatgc cgcgctggaa cgggccgacc tgtattttac cgaggattag tcttctgcgc
     4981 ggcggcgcgg cgctctgcgg ctgctgtatt cacctggtgc tttttttcac cgttaaatcg
     5041 cgaccattgc ataatttcat cgagcgtccg gtagcacccg gtgcacaact cgcgcttctc
     5101 gtccatactg cacacagccg tgcagggtga agcgatcagc aattctgtga ttttctgtat
     5161 accctgggct attttctgcc ccatgggcag gtatacatgg ccgaagagcc gggtcatgca
     5221 attattgccc acagccttgg acaagtacca cctgaggtat cagtgtttgt tcaaggggct
     5281 atagtgtgaa aacctgacag cttaatggtg gtggccgtgc gcgccgtgcg cgtggccgtg
     5341 tgcgagttct tcagccgatg catcgcgtat cgcaaccacc ttgatgtcaa aggtcagtgt
     5401 tttaccggcg taggggtggt tgccatcgac cgtgatactg tcggcgccga ccgcagtgac
     5461 gagcgctggt attacacctt cttcgctctc gagctcgatc tgcatgccga cctgcatctc
     5521 ttctggtttt tccagctggt ccttggggat cgtcatcacc atgtcctggc tgaactcacc
     5581 gaatgcgtcg cgggggccca tttcgacttt tcctgaaaaa ccttctttct tgtcggtcag
     5641 gaatttttcg agtgccggca tcaggttctt gaaaccatga aggtagtcga gcgtgccgtc
     5701 ctccagcttg ccttttccgc tttcactcag ctcatagctg atcgtcacta ctttgtttct
     5761 ggctacctgc atttttcctg cttacacact cagttcgaga attttggttt gtttgccggt
     5821 ttcggcatcg agaaaataaa acgatatatc acccaggtca tcgcgggtga gttcgagctg
     5881 gcaacggctc tggttcaggt taacggcttc taccagctcg ccatcgcagt aaagcgccat
     5941 gtgccctgtg ctggctgccg aatctttgcg gcggagcgac cagtccatga gcagcgattg
     6001 gtcgttggcg ctgattttca ggtcaaagat gacgcctggc aattcaggct tgagaacagt
     6061 aaagctcccc gctttgagaa aacctggttc gttatcgccc cggaaagcgg gcatcggtgt
     6121 cactgaccac gggctcaggg atacgctgtc ggtgtctaca cggaaggaat aacttgcctg
     6181 cagtagctgc agcttgatac ggttgtagag cgtgttccag acttcgtcgt cgagcacgaa
     6241 ctcctcgtcg ccgagaatat cccaggcatt ggcaatggta tgctcagaga cgagtgcggc
     6301 aaaaggcgaa agtgcttcgg tggccggggc cgatggctgg acaaagtcaa ccgacggggc
     6361 catatcaggt ttcgcaggct tttttgcccg ggccacagtc tttttgggct tttttaccgg
     6421 cagtaccggc ttgcgggcgc gtaacacagc cttttttgcc ctgcgggagg gggttttggt
     6481 cttacccgag cgtttaatct tcaattacgc ctctgacttt tcctctgtat ttccaacaag
     6541 tcttgttttt tccaccgggc gtaaaccgac tttagtgttt ctgtcgccgc cttgagctgt
     6601 cgctgcgcgg tggcaataga gtcgccggtt acctccgcca cgatacgtat aggaatgcgg
     6661 gtgtccatgg aaaaaaactt tcgccgcgca ctctcccggc gcttcttcag ggcctgcagc
     6721 gcggccgtat tggccgtccg ttcatgctgt tcggtgcgca tcaggatctg cagcaggcgg
     6781 tcgcgttcgc gaatgaaggc gactcgcttt tcttctgcgt atctcaggaa ggcgcggtaa
     6841 gacctcagca tcttccagtg cgaaccgtct ttctggcgac cccgttgcag caagtaccgc
     6901 agctccccat aggtcagggg gcaggccagt gcgagccttg cataagccga ggttggtggg
     6961 cgcatttttg ccagcagcgg cgaaagcgga tcaggtgtgg gattttccct gtactgcagt
     7021 tcaagccgcc gttcggccag ctcttcgacc gaaagtagct ccacctggga gcgcggcatt
     7081 tttcgccgcc ggagaaaatg gctgaattca aaattcagcg atgaagccat gtactggtag
     7141 aagggcagac ggcgcgcgtc gtagcgctgc agaataccgt cgagtttgcc agcgacgtgc
     7201 aggtagaagt cggccgaaac gtcggttgat tgctggtggc gcagctgggc atagttgtag
     7261 atgagcaggt ggagt
//
LOCUS       DAOQJF010000153         7253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5680_length_7253_cov_5.864824, whole genome shotgun sequence.
ACCESSION   DAOQJF010000153 DAOQJF010000000
VERSION     DAOQJF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5680_length_7253_cov_5.864824"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..657)
                     /locus_tag="PLF85_09220"
     CDS             complement(142..657)
                     /locus_tag="PLF85_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNE19888.1"
                     /translation="MRISVVQGDITQQQVDAIVNAANAQLAGGGGVDGAIHRAAGPEL
                     MQYCDTVRALEGGCPTGSAVITPAGRLPCKYVIHAVGPVWHGGGKNEDELLASAYRKA
                     LLLAEEHKLRTIAFSNISTGVYGFPKERAAKIVAKVMAEFALRVKFLEEVRFVCHDAQ
                     NLRLYRQAFNS"
     gene            752..1738
                     /gene="phnD"
                     /locus_tag="PLF85_09225"
     CDS             752..1738
                     /gene="phnD"
                     /locus_tag="PLF85_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE19889.1"
                     /translation="MAARVPHVTLLGLLLLFSQCGGQSSSLNPDLIAGKPEEVYGQPD
                     RQRKILKDEPQYKRSMNFGVTPWGELDKMQRAYEPLTDYLSEKLQVRIRLMIVQEYSE
                     LLADLRRGLVDIASFSPGAYADALDVGIDKESLYVASAQLGGKNYYQGIIISKQEIRS
                     LDALRGKTFAFVEEGSSSGYKFPLALLLGRRIDPYSFFSRVYFLGSHPNVVEAVIGGK
                     ADAGATWDGYIEENFPKQKPPVHILMRTEPIPYDAVVVGKKHGERFTQKVRRLLVGIN
                     KSTVTTEGVKILGSDSGFPYSGYVVNSNSMYDVVRKTSKLVRSYRRPEGATP"
     gene            1735..3774
                     /locus_tag="PLF85_09230"
     CDS             1735..3774
                     /locus_tag="PLF85_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE19890.1"
                     /translation="MKQQHTGVSRLLALLDERIRTPQFIRKRIEKYSVASKINFIILV
                     AVLPMLFILSVIFLVLFFQISGQIESTLLQKMVAAKNAYNFYERSTLVYAQMLSENAA
                     IKKELLADTVNVGPILRICRQAQAGTLLNRITVYDRKGIVIVRSHNPSEFGDDRSAEN
                     FVARALKEGHSSPALVHENGKLVLQNTVPIQFDDDRIGAVTAGYELDHQFARTLSDLT
                     QSGIFVVHAGQLVATSFGPDTGSVTDTRYETQNQDSSVSRRVNYTDRQNKQHSDSMDF
                     RFIPIATPAGSPDLRAPGLAIALKPSYPRFLLYTLFWGGFVLILLVIFLGVLLSFKIG
                     HKIANYASSLSEAMENFTKGDHDVRVSGASDDELGKVARSFNHMAEELQRRIQYIHDT
                     NESLAALIAERTQDLGEALNRVTRLEEMQQGDYLLMSQLLNPLSASDMAVGKLKANFI
                     AEQKKHYSFKGQSGEIGGDYTLFATLKFRGSRAQWLFVFNGDAMGKSSQGAAGALVCG
                     VTLQSILARAGTNPIAAKPEAWLKSVFKELDNIFSMFKGSMSMTAVLGLVNAQNGKAL
                     CINAEHPPLVLYRKNKARYADEKGRLRKLGGASAVTAVKPQVINLRPGDALVCGSDGK
                     DDLEIDGNIDASEERFLRVVEKSRADLRKIDAALRAAGRFTDDVSLVRIAFSSEK"
     gene            3816..4601
                     /locus_tag="PLF85_09235"
     CDS             3816..4601
                     /locus_tag="PLF85_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE19891.1"
                     /translation="MIAFPGDTYVTPADQKTDWQDFALLRSRWWLYAKFVSTVFKSRK
                     LIDDNRYGNENWAETSRDVMKAIESVGGRFEISGIENLRRAEGPLVIVANHMSALETF
                     VLPCLVTLIKPTTFVVKEKLMRGVFFGKIMKSRDPIVVGRENAREDMEIVLREGERIL
                     RGGKSVILFPEGTRQADFKPENFNSLGTKLAKRAGVNILPVALKTDFWSNGRILKGFG
                     PLKRRRRIHIAFGEAMPVEKKEKETHQKIIEFISSKFAAWKSA"
     gene            complement(4598..5128)
                     /locus_tag="PLF85_09240"
     CDS             complement(4598..5128)
                     /locus_tag="PLF85_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNE19892.1"
                     /translation="MKTADPNFIGKGIPDMSNVQVMSYLITTHHEFTRNIMDEIGELI
                     HEASLELVEPPTELSELAKMWKKYHEEMVMHLHDEENILFPWIEQLDQSGKTSEEITR
                     AYSGSVKQMLEEHKHHEEDMEKVKLLADKLSQKGGYAPVLARLAYKLKQLNQDLQEHM
                     EIESSLLFPRILGKKA"
     gene            5302..5964
                     /locus_tag="PLF85_09245"
     CDS             5302..5964
                     /locus_tag="PLF85_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE19893.1"
                     /translation="MSIKEAMEKSETIRMFENPLLERLSHVHPATPFVIYIPVIGVLL
                     YFAITATALSAGEIAAAFAAGMLFWTFIEYFLHRFIFHIPQTNKVFKAIYFYSHGIHH
                     DAPRDATRLVMPPGASIPLAVALFFLFRYASPVYYLPFMGGFITGYVIYDFLHFSVHF
                     FQFKSAYFKMIRRNHMMHHYRAPRKNFGFTSPFWDYVGGSYFGSTAADRNVDDHSGLK
                     PL"
     gene            6103..>7253
                     /locus_tag="PLF85_09250"
     CDS             6103..>7253
                     /locus_tag="PLF85_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNE19894.1"
                     /translation="MKIPELLMPAGSPHKLRVAIEYGADAVYCGVPRYSLRARENEFR
                     LHNLGEAVEFVRARGKKIYFTVNAIPRNSKVPGYLRYLKAMAELKPDGLIMADPGLIM
                     ITRDNFPDLPVHISVQANVMNFAAVEFWHRFGAKRVILSREVTLAEVREIRQRCPEME
                     IEVFVHGSICIAHSGRCFMSNYFKNRDANQGACNNACRDSYQIYLKNPEKEDAQMELI
                     SSEEGTFLMNSKDLRAIEFLDELAEIGVDSIKVEGRTKNDYYAAMIARTYRRALDDIR
                     DGKSFDRNLLTELDKVASRKYFSGFLTRGMDDRVPEDERDFQNEEGNTRYQSHYYAGC
                     VKRQDADGTAWFDLKTRLTEGDTVEVFFPGQFASRLFTAGKILRGGTEH"
BASE COUNT         1682 a         2044 c         1925 g         1602 t
ORIGIN      
        1 aaagtatcta ccatccctgt ggtaacggca gtggcttcca ctttatacca cggaactcac
       61 agacgaattg gggcgggggc ggcgctgcaa atctcgacct cgaactgcta gtgcgctagc
      121 ggctcctttt tcaggagcct gctagctatt gaaggcctgc cggtacaatc gcaggttttg
      181 tgcatcatgg cagacaaagc gcacttcttc caggaacttt acgcgtagcg caaactctgc
      241 catgaccttc gcgacaatct tagccgcacg ctctttagga aatccataca cccccgtgct
      301 gatgttagag aaagcgatcg tgcggagctt gtgctcttct gcgagcagca gcgccttgcg
      361 gtaggctgac gcgagcagtt cgtcttcatt cttgccgccg ccatgccaga ccggccctac
      421 tgcgtggatt acgtatttac atggcagtct tcctgccggc gtgatgactg ccgaacccgt
      481 gggacagccg ccctcaagcg cgcgcaccgt gtcacagtac tgcatgagct cgggcccagc
      541 tgcgcggtgt atagctccgt cgacaccgcc gccacccgca agctgtgcat tggccgcatt
      601 cacaatcgca tcgacctgct gctgcgtaat atctccctga acgaccgaaa tccgcatggg
      661 gcaaagaaac ctgcgcagag agtctgtaaa ccagatggat gtgaagtttt ctttatggtt
      721 gcatttcggg cgcacgaacg caggttgcac catggcagcc cgtgtgcccc acgtcactct
      781 gctcggcctg ttgctgctgt tctcgcaatg cgggggccag tcttcttcgc tcaaccctga
      841 cctcattgcc ggaaaaccgg aagaggtcta cggccaacca gaccggcagc gaaaaattct
      901 gaaagacgag ccgcagtata agcgcagcat gaacttcggc gtcacgccct ggggtgagct
      961 cgacaaaatg caacgtgcat acgagccgct caccgactac ttgtccgaaa aactgcaggt
     1021 gcgcatacgg ctgatgatcg tgcaggagta cagcgaactg ctcgccgacc tgcgccgggg
     1081 tttggtcgac attgcctcgt tctcgccggg ggcatatgcc gacgcactcg acgtgggtat
     1141 cgacaaagag tcgctctacg tggcgtcagc gcagctcggt ggtaagaatt actaccaggg
     1201 aatcatcatc agcaagcagg aaatccgctc gctcgatgcg cttcgcggca agacgttcgc
     1261 ctttgtcgaa gagggctctt cttcaggcta caagtttccg ctggcgctgc tgctggggcg
     1321 ccgcattgac ccctacagct ttttctcgcg ggtatacttc ctggggtcac acccgaatgt
     1381 ggtcgaagca gtgatcggcg gcaaagcgga cgccggcgcc acctgggacg gttacatcga
     1441 agaaaacttt cccaaacaaa aaccacctgt acacatactc atgcgcaccg agccgatacc
     1501 ttacgatgcg gtcgtcgtgg gaaaaaagca cggcgaacgc ttcactcaga aagtgcgccg
     1561 gctgctggtg ggcatcaaca agtctacagt aaccaccgag ggcgtcaaaa tactgggtag
     1621 cgattcgggt tttccttatt caggttacgt ggtgaacagt aacagcatgt atgacgtggt
     1681 acgcaagacc agcaaactcg tccgctctta ccgccgtccc gaaggagcaa cgccgtgaaa
     1741 cagcaacaca cgggcgtatc acgcctgctc gcactgctcg acgagcgcat ccgcacaccg
     1801 caattcatac gcaagcgcat tgagaaatac tcggtcgctt cgaagattaa cttcattatt
     1861 ctcgtggccg tactgccgat gctttttatt ctttcggtga tatttctcgt cttgtttttc
     1921 cagatcagcg ggcagattga atcaaccctt ctgcaaaaga tggtcgccgc aaagaacgcc
     1981 tacaacttct acgaacgcag caccctggtc tatgcgcaga tgctctcaga aaatgcggca
     2041 atcaagaaag aactgctcgc cgatacggtg aatgtcgggc ccatattgcg catctgccgc
     2101 caggcccagg caggaacact gctcaaccga atcaccgtct atgaccgcaa gggcatcgtg
     2161 attgtacgca gccataatcc ctcagaattc ggcgatgacc gctcagccga gaacttcgtt
     2221 gccagggcac tgaaagaggg acactcgagt ccggcgctgg tgcatgagaa cggcaaactg
     2281 gtcctgcaga ataccgtacc gatacagttc gacgacgacc gcatcggcgc tgtgaccgcg
     2341 ggctacgagc tcgaccacca atttgcccgc acgttgtctg acctgacaca gtccggcatt
     2401 tttgttgtcc atgcgggcca gctggtcgcc acttcatttg gccccgatac aggaagcgtg
     2461 acggacacac ggtatgaaac gcagaaccag gatagctccg tctcgcgccg cgtgaactat
     2521 accgaccggc aaaataagca gcacagcgac agcatggact ttcgcttcat tcccattgcc
     2581 acaccggcgg gttcacccga cctgcgggca ccgggactcg caatcgcgct caaaccctct
     2641 tacccgcgtt ttctgctcta tacacttttc tggggcggct tcgttcttat tctgctcgtc
     2701 atttttctcg gcgtactgct ttccttcaaa atcggtcaca agattgccaa ctatgcgagc
     2761 tccctttcag aggcaatgga gaacttcacg aaaggcgatc atgatgtgcg cgtgagcgga
     2821 gcatcagacg atgaactcgg caaagtcgcg cggtcgttca accatatggc cgaagagttg
     2881 cagcgccgta tacagtatat ccacgacacc aacgaaagcc tcgctgcact gattgccgaa
     2941 cgcacacagg atctcggcga agcactcaac cgcgtaacgc ggcttgaaga aatgcagcaa
     3001 ggcgactacc tgctgatgtc gcaactgctg aacccgctca gtgcatcgga catggcggtc
     3061 ggtaaactca aagcgaattt catcgctgaa cagaagaaac actatagttt caaaggccag
     3121 tcgggagaaa taggcggcga ctacacactc tttgcaacac tgaagtttcg aggttcacgg
     3181 gcgcagtggc tctttgtttt caacggcgac gcgatgggta aatcaagcca gggggctgca
     3241 ggtgcgctgg tctgtggtgt gacgctgcaa tcgattcttg cgcgcgcggg tacaaacccg
     3301 atcgctgcga aacccgaagc ctggctgaaa tccgtattca aagaactgga caatattttc
     3361 agcatgttca agggctcgat gagcatgact gcggtgctgg gccttgtcaa cgcacaaaac
     3421 ggcaaagcgc tctgcatcaa cgctgaacat cccccgctgg tgctctaccg aaagaataag
     3481 gcgcgttatg ctgatgaaaa aggcaggctg cgaaagctgg gaggagcttc ggcggtgacg
     3541 gctgtgaaac cccaggtgat caacctgcga ccgggcgatg ccctggtctg tggctcagac
     3601 ggcaaagacg acctggaaat tgacggcaat atcgatgcca gtgaagaacg gtttttgcgc
     3661 gtagtcgaga aaagccgcgc cgacctgcgc aaaatcgacg cggcattgcg ggcggcaggc
     3721 cggtttaccg acgatgtttc gcttgtccga attgccttta gcagtgaaaa ataaaaattc
     3781 gcaaccttga ggcgccgcgg cgcatccaaa ttggcatgat cgcttttcca ggggacacct
     3841 atgtcacgcc cgccgaccag aaaaccgact ggcaggattt cgccctgctg cgctcgcgct
     3901 ggtggctcta tgcaaagttt gtcagcactg tttttaagtc ccgcaagctg atcgacgaca
     3961 accgttacgg caatgaaaac tgggcagaaa cttcgcgcga cgtgatgaaa gccattgaat
     4021 cggtcggcgg ccggttcgaa atcagcggta tcgaaaacct gcgccgcgct gaaggcccgc
     4081 tcgtgatcgt cgcaaaccac atgagcgcgc tcgaaacttt tgtactaccc tgtctcgtga
     4141 cgctgatcaa acccacgact tttgtcgtga aagaaaagct catgcgcgga gtttttttcg
     4201 gtaagatcat gaagtcccgc gacccgattg tcgtggggcg cgagaacgca cgtgaggata
     4261 tggaaattgt gctgcgcgag ggcgaaagaa tcctgcgcgg gggaaaatcc gtcatcctgt
     4321 ttcccgaagg cacccgccag gcagacttca aacctgagaa cttcaattca cttggtacca
     4381 agctcgccaa acgggcgggg gtcaatattc tgccggtcgc gcttaagacc gacttctgga
     4441 gcaacggccg tatactaaaa ggctttggcc cgctgaaacg ccggcgccgt attcacatcg
     4501 ccttcggcga agccatgccg gtcgaaaaga aagagaaaga gacgcaccag aaaattattg
     4561 aattcatctc ttcaaagttc gcagcctgga aatcagctta agctttcttg ccaagaatcc
     4621 gcgggaacag taacgagctt tcgatctcca tgtgttcctg caggtcctgg ttgagctgct
     4681 tgagcttgta ggcaagccgt gccagcacgg gtgcgtaacc gcccttctgt gacagcttgt
     4741 cggcgagtag ttttactttc tccatgtctt cttcatggtg cttatgctct tcaagcatct
     4801 gcttgaccga acccgaatag gcgcgggtga tttcttcgct ggtttttccc gactggtcaa
     4861 gttgctcgat ccacgggaag agaatattct cttcgtcgtg caggtgcatg accatttctt
     4921 cgtggtactt cttccacatc ttcgcaagct cagaaagttc tgtgggtggt tcaaccagct
     4981 caaggctggc ttcgtgaatc agctcgccaa tttcgtccat gatgttgcgc gtgaattcat
     5041 ggtgcgtggt gatcaggtaa ctcatgacct gaacattgct catatcgggt atgcccttac
     5101 cgataaaatt aggatccgct gttttcatac aaagctcctt gcctcggccc gttcggggcc
     5161 atacctacag cgggcaatta cacttaggct gtcaattaat aacagaatac cggagccatt
     5221 agtccttatt tgtggttgac aacctaagcc cgcgctgcca tcgggctaaa attccacgag
     5281 cgaggtatta tggcaaaacg catgagtatt aaagaggcga tggaaaagag cgaaaccatc
     5341 cgcatgtttg aaaatccttt gctagaacgc ctgagccacg tgcacccggc aacgccgttc
     5401 gtgatctaca tacccgtgat tggcgtactg ctgtatttcg ccataacggc gactgcgctt
     5461 tccgccggcg aaattgccgc tgcgtttgcg gcaggtatgc tattctggac attcatcgag
     5521 tattttctcc accgctttat cttccacatt ccgcagacca ataaagtctt taaggcgata
     5581 tatttctatt cgcacggcat ccaccatgac gcgccgcgcg acgccacgcg cctcgtgatg
     5641 ccacccggtg caagtattcc actcgccgtg gctcttttct ttttgttccg ttatgcctcg
     5701 cctgtctatt acctgccttt catgggggga ttcatcacgg gttacgtcat ctacgacttt
     5761 ttgcattttt ccgtacattt cttccagttc aagagcgcct attttaagat gattcgccgt
     5821 aaccacatga tgcaccacta ccgcgcaccg cgcaagaact ttggtttcac ctcacctttc
     5881 tgggactacg tcggcggttc gtatttcggc agcacagcag ctgataggaa tgtcgacgat
     5941 cacagtggtt tgaagccctt gtagtttaat ctgtcatgct gaggagcttc tggctacgct
     6001 tcgagacgaa aaatccgcgc tgcggatttt tccctcagcg tgacgccaga agcgtcccga
     6061 agtatgactc ggcagccacc cacgattatg tggcaccata caatgaaaat acctgaactc
     6121 ctcatgcctg ccggcagccc gcacaagttg cgggtggcaa ttgaatacgg cgccgacgcc
     6181 gtgtactgcg gtgtgccgcg ttattcgctg cgtgcgcgcg aaaacgaatt tcgcctgcac
     6241 aacctcggcg aagccgtcga atttgtgcgc gcgcggggta aaaagatcta cttcactgtc
     6301 aatgcgatac cgcgcaactc gaaagtgccg ggctacctgc gctacctgaa agcgatggca
     6361 gagcttaaac ccgacgggct gatcatggcc gacccggggc tgatcatgat cacgcgcgat
     6421 aattttcctg atctgccggt gcatatctcg gtacaggcaa acgtgatgaa tttcgcggcg
     6481 gtcgaattct ggcacaggtt tggagccaaa cgcgtgatac tctcgcgcga ggtaacactc
     6541 gccgaagtgc gggaaatccg ccagcgttgc cccgaaatgg aaatcgaagt ttttgtacac
     6601 ggttcaatct gtattgcaca cagcggccgc tgcttcatga gcaactattt caagaaccgc
     6661 gacgcgaacc agggcgcgtg caacaatgcc tgccgcgact cttaccagat ctacctcaag
     6721 aacccggaaa aagaagacgc gcagatggag ctcatcagtt ctgaagaagg cacgtttctg
     6781 atgaactcga aagacctgcg cgcgatagag tttttagacg aactcgccga aatcggcgtc
     6841 gactcgatca aggtcgaagg ccgcacgaaa aacgactact acgccgcgat gattgcgcgc
     6901 acctaccggc gggcgctgga cgacatccgc gacggcaaat cgttcgaccg taacctgctc
     6961 accgagctcg acaaggtcgc gtcgcgtaag tatttttcag gcttcctcac ccgcggaatg
     7021 gatgacaggg tacccgaaga cgaacgcgat ttccagaatg aagagggaaa cacgcgctac
     7081 cagtcgcatt actatgctgg ctgcgtgaaa aggcaagacg ccgatggcac ggcgtggttt
     7141 gatctcaaga cacgcctcac cgaaggtgat actgttgaag tattctttcc cggccagttt
     7201 gcctcgcgct tgttcactgc gggtaaaatt ctgcgtggcg ggactgagca cat
//
LOCUS       DAOQJF010000154         7237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5694_length_7237_cov_5.950849, whole genome shotgun sequence.
ACCESSION   DAOQJF010000154 DAOQJF010000000
VERSION     DAOQJF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5694_length_7237_cov_5.950849"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLF85_09255"
     CDS             <1..467
                     /locus_tag="PLF85_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19895.1"
                     /translation="VEYCFRPMENLVAGVRARRLTARQLVYESIGTFIRFARRHRRYF
                     LFLTKERLGGKAKVRGRIRERTREMAMILGHDLIGLPEWQGVPISLAQDVAEMIMDQM
                     MLMLDEALDLPPGESNTEVKVIARARKKVWILLRGGLSYKDRHGRLGAPTGT"
     gene            complement(499..912)
                     /gene="atpC"
                     /locus_tag="PLF85_09260"
     CDS             complement(499..912)
                     /gene="atpC"
                     /locus_tag="PLF85_09260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNE19896.1"
                     /translation="MENTAAQLTVSIYSPEKKLLEEPAVSVGLPGKKGAFTVLKGHAN
                     LVAELEAGIVHVMTGEREELYVVDSGFAEVKGNQVIVLVEGATDPKTLKAADERAALE
                     EALNRPTSNPVQYAKKQHDIALRRVRARIAESGTH"
     gene            complement(952..2343)
                     /gene="atpD"
                     /locus_tag="PLF85_09265"
     CDS             complement(952..2343)
                     /gene="atpD"
                     /locus_tag="PLF85_09265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNE19897.1"
                     /translation="MAVVGKVSQVIGSVLDAKFPEGKLPEIYNALELQVQGQKLVCEV
                     QQHLGGGAIRAVALGSTDGVARGQDITDTGAPISIPVGLNSLGRIFNVLGEPVDEAGP
                     VKVEERRPIHRPSPGFEDIEPKTQIFETGIKVIDLLEPYIRGGKTGLFGGAGVGKTVI
                     IMELINNIAKQHGGYSVFAGVGERTREGTDLFLEMQESGVIDKTCLVYGQMNEPPGAR
                     LRVALTALTQAEWLRDYAGTDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAT
                     EMGQLQERITSTKKGSITSVQAIYVPADDITDPAPATAFTHIDASTVLSRKISEKGIY
                     PAVDPLSSTSRALSPGVVGERHYSIAREVQRILQRYKDLQDIIAILGMDELSEDDKVL
                     VGRARRIERFLSQPFHVAEAFTGMQGKYVKLEDTIRSFEELLSGKYDKYPEQAFYMCG
                     AIEEVVEKAKSMG"
     gene            complement(2356..3222)
                     /gene="atpG"
                     /locus_tag="PLF85_09270"
     CDS             complement(2356..3222)
                     /gene="atpG"
                     /locus_tag="PLF85_09270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNE19898.1"
                     /translation="MATSKEIRNRIGSVQNTKKITRTMELVSTAKAKKAVDKVVASRP
                     YADKISELIKSLAAQGEVADHPLLRKHPHVRRALVVVVTANRGLCGGYNANVLRLAMQ
                     QIEDYKKAGVEVIVHLIGKKAISTFNYLKIPFAASFTHLDDKLTFADAQKFAEDYMER
                     FSLEQVDKIDLIYTRYMSSAVQKPVSDTILPVEIPASEKAHKTLPFIFEPDPQSILQS
                     LLPKAICQNLYQSLLDAMASEQIARRIAMKNATDAASDMIKALTKEYNRVRQAKITQE
                     IAEIVGGAAAIQ"
     gene            complement(3249..4775)
                     /gene="atpA"
                     /locus_tag="PLF85_09275"
     CDS             complement(3249..4775)
                     /gene="atpA"
                     /locus_tag="PLF85_09275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNE19899.1"
                     /translation="MKINTSEIVSVLKSEIQNYGQTLTIDEVGQVLEVGDGIARVYGL
                     SNALAGEMLEFENGITGLAFNLEESSVGVVILGDYLKIREGMSIKRTGRILEVPTGDA
                     LIGRVVNPLGQPLDGKGPIVSKTTRPVEIIAPGIARRKPVGEPMQTGIKAIDAMIPIG
                     RGQRELIIGDRSTGKTAIAIDTILNQKGTGVICVYVAIGQKSSTVAGVIDKLKEHGAL
                     DYTIVVAANASDPAPMQYLAPYSGTAMAEEFMYNQGKATLCVYDDLTKQANAYRQMSL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSDKYGGGSLTALPVIETQEGEVSAYIPTN
                     VISITDGQIYLQPNLFAAGVRPPVDVGISVSRVGGSAQIKAMKKVAGTLRLDLAQFRE
                     LEAFAQLGTELDPATQRQIDRGQRLVELLKQPQYKPMPVEKQVVSIYAATKGFMDKVP
                     VKNIRDYEDKLLEFIEKTHNEFYVTIRDTKGLPDEGKLNSVLQDFTDSYLQNKSGDPR
                     "
     gene            complement(4791..5402)
                     /gene="atpH"
                     /locus_tag="PLF85_09280"
     CDS             complement(4791..5402)
                     /gene="atpH"
                     /locus_tag="PLF85_09280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNE19900.1"
                     /translation="MIFNKVAHNYALALSQVQGLNLEQAEEDLHAVRDMLRTDQKFSV
                     FVQSPSISRDIKKNAFRKALEGKVQKAVLSICMILIERRRMELMPELFVSFRHIVDEL
                     LGRTYIDVTVAKTLNTNETPLDEALRDSVIKKIDENRAAFGLPKDKKLNYTMSVKIDP
                     ELLAGIRVRVGDYIFDGSVARNLIKWHDVAAVHPLDVAKAFTE"
     gene            complement(5399..5917)
                     /gene="atpF"
                     /locus_tag="PLF85_09285"
     CDS             complement(5399..5917)
                     /gene="atpF"
                     /locus_tag="PLF85_09285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNE19901.1"
                     /translation="MVLLTGTSIDLLKVSPGLVFWTAVTFILVLVVLWLFAWKPIIQG
                     LDARNDKIQEDLDNSRKLREQAESLMADYKKHLDAAKDQALGIIDEGKRDAESIRHRI
                     LEDAKKEAEEIHNRAVGEISQAKAKAIKELEKEVVDLSVAIISKILSRDVSKEDHRNI
                     ILKELDQLKSGK"
     gene            complement(5971..6174)
                     /locus_tag="PLF85_09290"
                     /pseudo
     CDS             complement(5971..6174)
                     /locus_tag="PLF85_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
     gene            complement(6194..7222)
                     /gene="atpB"
                     /locus_tag="PLF85_09295"
     CDS             complement(6194..7222)
                     /gene="atpB"
                     /locus_tag="PLF85_09295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNE19902.1"
                     /translation="MFLGAIALVSFCPTALSANPAPAKAGAPAAPATAVPAADAHAAG
                     EAAGAEHAAGSDAFSFAETIAHHLSDAPLWKLELAGYDISITKRVVMLFLAAALLILI
                     LVPAARRIAKDPYKKPSRFTGFVEVLVNFIRHDVAHASLGHHSHPYEPYLLTLFFFIL
                     ISNLLGLIPSLGEIYVFVGQATGLMHAHAGHADSLSLPLAEKLWPGITATGDIGVTAT
                     LAIFTFIVILISGFAYQGVLFIRNIVPDGIPLLLWPIMWPIEFIGLFTKPFALAIRLL
                     ANMTAGHMIILVLLGFIFQFQSYFIAPVSIAGAIAIYLLEIFVAFLQAYIFTFLTSLF
                     ISQVQHRH"
BASE COUNT         1643 a         1930 c         1927 g         1737 t
ORIGIN      
        1 tggtagaata ctgctttcgc ccgatggaaa acctggtagc gggtgtccgc gcgcggcggc
       61 tcacagcgcg gcaactggtc tatgaatcga tcggcacctt cattcgcttc gcgcgccggc
      121 acagacgtta tttcctcttt ctgaccaaag agcggctggg gggaaaagcc aaggtgcgcg
      181 ggcgaatacg cgagcgcacg cgcgaaatgg caatgattct cgggcacgac ctgatcggct
      241 tacccgaatg gcagggtgtg cctatctcgc tcgcgcagga cgtcgctgag atgatcatgg
      301 accagatgat gctgatgctc gacgaagcgc tcgatttgcc cccgggggaa agtaataccg
      361 aggtaaaggt cattgcgcgc gcgcgcaaaa aagtctggat tctgctacgg ggtgggttgt
      421 cttacaaaga ccgccatggc aggttgggag ctcccacagg aacctaaacc tgcttttggc
      481 tcctttttca ggagcctgtt agtgagtacc tgattctgca atacgcgcac gcacgcgacg
      541 cagcgcgatg tcatgctgct tcttggcgta ctgcacgggg ttactcgttg ggcggttcag
      601 cgcttcttcg agcgccgcac gttcatctgc cgctttcagg gttttgggat ctgtcgcgcc
      661 ctcgacgagc acaatcacct ggttgccttt gacttcggca aaaccagaat caacgacata
      721 aagttcttcg cgctcaccgg tcatcacgtg gacaataccc gcttcgagtt ctgcgaccag
      781 gttcgcgtgg cctttgagaa cagtgaatgc tcccttttta cccggcaaac cgaccgatac
      841 cgccggctct tccagcaact tcttttccgg tgaatagatg ctgactgtca gctgtgccgc
      901 ggtattttcc actggttttc ctcttagtta gtctgcgcga aaagccgggc cttagcccat
      961 gctcttcgct ttttcaacaa cctcttcgat cgcaccgcac atgtagaatg cctgctcagg
     1021 gtacttgtcg tatttgcctg agagcagctc ttcgaacgag cggatagtgt cttcgagttt
     1081 cacgtatttg ccctgcatac ctgtgaaggc ttcagcaacg tggaaaggct gtgaaaggaa
     1141 gcgttcaatg cggcgggcac ggcccacaag aaccttgtcg tcctctgaga gttcgtccat
     1201 accgagaatc gcgataatgt cctgcaggtc tttgtaacgc tgcagaatac gctgtacttc
     1261 gcgcgcaata gaatagtggc gctcaccgac aactcccggc gaaagagcgc ggctggtcga
     1321 agaaagcggg tcaaccgctg ggtaaattcc cttttcagag attttacgcg aaagaaccgt
     1381 tgatgcatca atgtgggtaa acgcggttgc aggtgccggg tcggtgatgt cgtcggcagg
     1441 aacgtaaatc gcctgaaccg aagtgatcga tcctttcttg gttgaagtaa tacgctcctg
     1501 cagctggccc atttcggttg ccagtgtagg ctggtaaccc accgcagaag gcatacggcc
     1561 gagaagtgct gacacttctg aacccgcctg ggtgaagcgg aagatgttgt cgacgaacag
     1621 cagaacgtct gtacctgcgt aatcacgcag ccactcagcc tgtgtcaacg ctgtcagcgc
     1681 aacgcgcaga cgcgctccgg gtggctcgtt catctggccg tacacgaggc aggttttgtc
     1741 aataacacct gattcctgca tttcgagaaa gaggtcagta ccttcacgcg tgcgctcgcc
     1801 tacaccggcg aataccgagt acccaccgtg ctgcttcgcg atgttgttga tgagctccat
     1861 gatgataacg gtcttaccaa caccagcacc accgaacaga cctgtcttac cgccgcggat
     1921 gtacggctcg agcaggtcga tcaccttgat acctgtttca aaaatctgtg tcttgggctc
     1981 gatatcttcg aaaccaggcg aagggcggtg aataggacgg cgctcttcaa cctttacggg
     2041 gcccgcttcg tctacgggct cgcccaaaac gttaaagata cggccgaggg agttgagacc
     2101 tacgggaatc gagattggcg ctccggtatc ggtgatatcc tggccgcggg ctacgccatc
     2161 ggttgaaccg agggcaaccg cacgaattgc gccgccaccc aggtgctgct gcacttcgca
     2221 gacgagcttc tgcccctgca cctgcagttc gagagcgtta taaatttccg gtaattttcc
     2281 ttcgggaaac tttgcatcga gtaccgaccc gatgacctga cttacttttc cgactactgc
     2341 catcttgttt tctccttatt gaattgcagc tgcgccgccg acgatctccg caatttcctg
     2401 cgtgattttt gcctggcgaa cacggttata ttctttggtc aatgccttga tcatgtctga
     2461 tgccgcatcg gtagcattct tcatcgcaat acggcgcgca atctgctctg acgccattgc
     2521 atcgagcagc gactggtaca ggttctggca gatcgccttt ggtaaaagag actgcagaat
     2581 agactgtgga tccggttcga aaataaacgg cagagttttg tgcgctttct cgctcgcagg
     2641 aatctccaca ggaagaatcg tatcagatac gggtttctga acagccgagc tcatataacg
     2701 agtataaatc aggtcaatct tatcaacctg ctcaaggctg aaacgctcca tgtagtcttc
     2761 agcaaacttt tgcgcgtcgg caaacgtcag cttatcatcc aagtgcgtga atgaagcggc
     2821 aaaaggtatc ttcaggtagt tgaaggttga aatcgccttc ttgccaatca ggtgcacgat
     2881 cacttctaca cccgctttct tgtaatcttc aatctgctgc atcgccagac ggagcacgtt
     2941 cgcattataa ccgccgcaga gcccgcggtt tgctgtcacc acgacaacga gcgcacgacg
     3001 tacgtgcggg tgcttacgca gcagcgggtg gtcggcaact tcgccctgtg ctgccagtga
     3061 ctttatcagt tcgctgatct tatcggcgta cgggcgcgag gccacaacct tgtcgactgc
     3121 cttctttgct ttggcagtcg acaccagctc catggtgcgc gtgatcttct tggtattttg
     3181 taccgagccg atccggttgc gtatttcttt tgaggtcgcc atgctctctt ctgaatcagg
     3241 tcaggcgact agcggggatc tccggatttg ttctgcaggt acgaatcagt gaaatcctgc
     3301 agaactgagt tcagcttgcc ctcgtctggc aggcctttcg tgtcacggat cgtgacatag
     3361 aactcgttgt gcgtcttttc gatgaattca agcagcttat cttcgtaatc ccggatattc
     3421 ttcacaggaa ctttatccat gaagccttta gtcgcagcat aaatcgaaac gacctgtttc
     3481 tccaccggca ttggtttata ctggggctgc ttcagcagtt caacgaggcg ctggccacgg
     3541 tcaatctggc gctgtgttgc cggatcgagt tctgtaccca gctgcgcaaa cgcttcgagt
     3601 tcgcggaact gtgcaaggtc gaggcggagt gtacccgcga ccttcttcat cgctttaatt
     3661 tgcgcggaac caccaacgcg ggaaaccgat ataccgacat ccacaggcgg acgcacaccg
     3721 gccgcaaaca ggttcggctg cagatagatc tgcccgtcag tgatcgaaat tacgttcgta
     3781 gggatatagg cagaaacctc accttcctgg gtttcgatta ccggaagtgc agtcagtgaa
     3841 ccaccgccgt atttatcgct gagttttgcg gcacgctcaa gcaggcgcga gtgcagatag
     3901 aatacgtcac ctggatacgc ttcgcgacct ggcggccggc ggagcagaag cgacatctgg
     3961 cggtaggcat ttgcctgttt ggtaaggtca tcatacacac agagcgttgc cttgccctgg
     4021 ttgtacatga actcttctgc cattgcagta ccagaataag gtgcaagata ctgcataggc
     4081 gccgggtcag aggcgtttgc tgcaacaacg attgtataat cgagtgcacc gtgctctttc
     4141 agtttatcga taacacctgc aaccgtagaa gatttctgac cgatggcaac gtacacgcag
     4201 atcactcccg tgcccttctg gttgaggatt gtatcgatag caattgctgt cttacctgtt
     4261 gaacggtcgc cgatgatcag ctcgcgctgg ccgcgaccaa ttggaatcat tgcatcgatt
     4321 gccttgatac ctgtttgcat tggctcgcca acgggcttgc ggcgagcgat tccaggagcg
     4381 ataatctcaa ccgggcgtgt cgtcttgctg acgatcggac ctttaccgtc gagtggctga
     4441 cccagcgggt taaccacgcg gccgatcagc gcatctcccg tgggtacttc aagaatgcgg
     4501 ccagtacgct tgatgctcat gccttcgcgg attttcaggt aatcaccgag aattaccaca
     4561 ccgaccgagc tctcttcgag gttgaatgca agaccagtaa tgccgttttc aaactcaagc
     4621 atctcgcccg cgagggcatt ggaaagaccg tagacgcggg cgataccatc accgacttcg
     4681 agcacctgac cgacttcgtc gattgtcagt gtctggccgt agttttgaat ttcgctcttc
     4741 agtaccgaga cgatttctga tgtattaatt ttcatatttt ccccgtcgtt ttattcagta
     4801 aaagcctttg cgacatcgag cggatgcact gccgccacat cgtgccattt aatcaggttg
     4861 cgagcaaccg agccgtcaaa aatataatca cctacacgaa ccctgatgcc ggcgagcagt
     4921 tcaggatcga tttttacgct cattgtataa ttgagctttt tatcctttgg tagaccaaag
     4981 gccgcccggt tttcgtcgat ctttttgatc acgctgtcac gcagcgcttc gtcgagcgga
     5041 gtttcgtttg tattgagagt tttcgccact gtaacgtcaa tgtaagtcct gccaagcagt
     5101 tcatcgacaa tgtgcctgaa cgaaacgaat agttcaggca tcagctccat acggcggcgc
     5161 tcaataagta tcatgcagat actgagcact gctttctgta ccttaccttc cagcgctttg
     5221 cggaaggcat ttttcttaat gtcccggcta atgctcggcg attgcacaaa caccgaaaac
     5281 ttctggtctg tccgcagcat atcgcggacg gcgtgcaggt cttcttcggc ctgttcaagg
     5341 ttaagaccct gaacctgcga aagcgcgagc gcgtagttgt gtgcaacctt gttaaagatc
     5401 atttgcctga tttcagttgg tcgagctctt tcagaataat attgcgatgg tcttctttag
     5461 agacatcgcg gctaagaatc ttggagatga tcgccacgct aaggtcaacg acctctttct
     5521 cgagctcttt aatcgccttg gctttcgcct gcgaaatttc gcccacagcg cggttatgaa
     5581 tctcttctgc ttcttttttg gcgtcttcga gaatgcggtg cctgatagac tccgcatcgc
     5641 gtttaccctc atcgattata cccagcgcct gatcctttgc agcatcaagg tgctttttat
     5701 aatctgccat gagagactca gcctgctcac ggagcttgcg cgagttatcg agatcttcct
     5761 gtatcttgtc gttacgcgca tcaaggccct ggatgatggg cttccatgca aataaccaca
     5821 ggacgacgag gaccagaata aaggttaccg cggtccagaa aaccagaccg ggggaaactt
     5881 taagcaggtc gatgcttgtt ccggtgagca gtaccatgct ttgagccgct taagcctgtt
     5941 cgatcaaact ttgatcttat cgagcatcag gaaggcaata acgagggcga acagagccgc
     6001 accttcgata agagccgccg caatgatcat ggctgtctgg atcttgttgg ctgcttctgg
     6061 ctgacggctt acaccttcag ctgctgcgcc accgatacgg ccgatgccga gagctgctcc
     6121 gacgactgcg aggccggctg cgagtcctgc tccgattgcg ataattgaac ccattgttaa
     6181 ctcctttgtt cttttaatgc ctgtgttgta cttgtgatat gaataatgac gtcaggaacg
     6241 tgaagatata cgcctgcagg aaagccacaa agatctcaag caggtaaatc gcaatcgcac
     6301 cggcgatact cacgggtgcg ataaagtatg actggaactg gaagatgaag cccagcagca
     6361 caaggatgat catgtggccg gctgtcatgt tcgccaaaag acgaatcgcg agcgcaaatg
     6421 gcttggtaaa gagaccgatg aactcgatcg gccacatgat gggccagagc agcaaaggaa
     6481 taccgtcagg tacaatattc cgaatgaaga gaactccctg gtaggcaaag cctgagatca
     6541 ggatcacgat gaatgtgaat atagccaggg tggcagtcac gccaatgtcg cccgttgcag
     6601 taatacccgg ccagagcttc tctgcgagag gcaggctcag cgaatcagca tggcctgcat
     6661 gcgcgtgcat cagcccggtt gcctggccga caaagacgta gatctcgccg agcgaaggta
     6721 tcaagccaag caggtttgaa atcaggataa agaagaagag cgtgagcagg taaggctcgt
     6781 aaggatgaga atggtgccct aggctcgcgt gtgccacatc atgacgaatg aagttcacga
     6841 gaacttctac gaaaccagta aagcgtgaag gcttcttata gggatctttc gcaatgcggc
     6901 gcgccgccgg cacaagaatc aggataagaa gagctgcggc aagaaagagc atcactacgc
     6961 gcttcgtgat ggatatgtcg taccccgcaa gctcaagctt ccagagcgga gcatcagaaa
     7021 ggtggtgagc aatggtctcg gcaaaactaa aagcgtcaga acctgccgca tgctctgcac
     7081 ccgccgcttc accggcagcg tgagcatcag cagccggaac tgcagtcgct ggagcagccg
     7141 gagctccggc cttagcagga gctggatttg ccgacagagc agtcgggcaa aaagaaacga
     7201 gggcgattgc cccgaggaag attgcgagaa ctgtgtt
//
LOCUS       DAOQJF010000155         7232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5702_length_7232_cov_4.854953, whole genome shotgun sequence.
ACCESSION   DAOQJF010000155 DAOQJF010000000
VERSION     DAOQJF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5702_length_7232_cov_4.854953"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLF85_09300"
     CDS             <1..537
                     /locus_tag="PLF85_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNE19903.1"
                     /translation="GMLYMSRALGQPLPELISTVSYAMNLIRIAHAKEYTVFLLGSRD
                     EVLEKLYTNFRRSFTGLRIVGRHHGYLKGEAGTRVLEAVRKTDPHILFVGMGYHRGMK
                     WIEQNRSQLGDLIVVNVGGAFDTLAGTRQKAPAFMASAGYTWLWRTINKPYRWHRFFM
                     VIYWFFEVTYYRFSKKNK"
     gene            complement(563..1405)
                     /locus_tag="PLF85_09305"
     CDS             complement(563..1405)
                     /locus_tag="PLF85_09305"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNE19904.1"
                     /translation="MPDYSAEHNALLPVVREAGHAVMRFFTRGGYEVQHKDPENPVTE
                     ADFAANRILVRAIRDLFPDDALLSEEIEHPHERGAMNAERSERKRVWIIDPIDGTREF
                     IKGRPQFAVSVGLVEAGRAVLGFICNPAADYLVSGGPCLGLFRDGKKFTPPHRPAFAP
                     PASYPRIVVSRSEFKQNQITHLQKYYEDLEKQAMGSIAYKLALVADGTFDLVVSVKPK
                     NEWDLAGGAALLAAEQLELRDGSYTPLVFNKAETETLGLIAGTKEACLWYKNIQDSQA
                     PTPE"
     gene            complement(1406..2818)
                     /locus_tag="PLF85_09310"
     CDS             complement(1406..2818)
                     /locus_tag="PLF85_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE19905.1"
                     /translation="MNSGPAPDDRLQNAWDSLAALVAQGRRVWPSDSEEFPQYLKDAT
                     FLKGQAALVLQPQNAEELRAIVIEAQVLRKALPQYSRALSLTLRGGGSGLSGACVPEG
                     GVVLDLTRMNRIIRIDEQNQSITAECGVILSELNTALAATGLRYAVDPSSSTLCTVGG
                     SVATNAAGPSSLKYGTTRSNLAAVTILTPTGDFVRAGALPVKTSMGFSLPDLFCGSEG
                     RLGIITEAEFRLLPQPEESALLVAAFASEIAAAEFILKLRRSSIRPAAIEILDYRSSS
                     LVDFPPGCPKGGALVLVELDGTAEEVAYDLGRLGQLAESHEFVAARDETSRQALWNRR
                     KAVSLEVSKLAPHRIGEDVAVPVSALPRAMEFARVQAAAQQVETAIWGHAGDGNLHVN
                     YLLQSGERLGVVLGLMRELAAEVTRLGGAMSGEHGLGRLKRGIARQILPKEYFAAQVS
                     IKNAFDPQHLLNPALEMEFN"
     gene            2873..3283
                     /locus_tag="PLF85_09315"
     CDS             2873..3283
                     /locus_tag="PLF85_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19906.1"
                     /translation="MRAWHKAAMIAILVSGCMSIDRKIFEAGCAPPRRTEREIAIGYA
                     LPLQKHHYLGEITIQYGSGYERDEILRRLRVEAAQCGADGLLLGSFSRVDSKWKWADK
                     NVNDSFDTSGYRLIVTLYRYDDSEKVAEPPAAKR"
     gene            complement(3284..4132)
                     /gene="mazG"
                     /locus_tag="PLF85_09320"
     CDS             complement(3284..4132)
                     /gene="mazG"
                     /locus_tag="PLF85_09320"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNE19907.1"
                     /translation="MARSAPQKNDLMKKPPQSTDPLADLLQVARDLRDKNGGCPWDLE
                     QSHESLAKHLLEEASELIEVIEHGLTPDTDEAFKEELGDVLFQVVIHAQLAAEEGRFA
                     FADVARTIADKLITRHPHVYGELQVADSAQVLKNWEAIKARERARKQKRTSYLDGVPK
                     ILPALQRAERLGEKAARTGFDWVNDRQLWEKIHEEIREFEAAESESRQRREEELGDLL
                     FALAQYSRRQGIDAEGALRKACVKFENRFRHMELTAGEKVAAGEHLSTEEWDSLWQQA
                     KNATAG"
     gene            4149..5918
                     /locus_tag="PLF85_09325"
     CDS             4149..5918
                     /locus_tag="PLF85_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE19908.1"
                     /translation="MRSLVTPYLLTLLSDWLLISLAYAWYFHATGQNLLIVPVFSGVQ
                     VAVLAWAKLYRANLRYLNLFSLRTLAIVAFLGSAATQLVSLPAGRYFVLEAVIAWFGQ
                     NTLTLFHRLFWYFRLNPEARLKKQGLTPIVIYGAGTVAYHLLQDLSAEDLLGKYQIAA
                     VVDNDPQKEGAHLGPYRVHAGKHLPTLAEKYQVKEIWFTMPVTPDFLKQVMDQLTGMS
                     IAYKVIPRKLAQFAPDIRNLRIEDLIQRPEIRLDPGPVERAIRGRRVLVTGAAGSIGR
                     ELSRQIAALGPARLTLIDQWEHGVYQLEQEFNGENIFCAIADVRNEKRLADIITQEKP
                     ELIFHAAAYKHVPLMETNHLQAIDTNIMGTWHLLAAALRYAAGLPQGQQVRMVNISTD
                     KAVQPENIMGISKRMAELLVFNAARESDGRLKTTSVRFGNVLGSSGSVVPLFWDQIQR
                     GGPVTVTHPEMERFFMTIPEAVRLVLQAAALEEETDCILALDMGKPVRITELAERLIL
                     LAGKKPGSEIAITYSGIRPGEKLKEELFWETNSVRTAIPFIFRSTADLKEMDIQGFMG
                     LLQSALEENRSRSWYREFLLRYV"
     gene            complement(5909..6916)
                     /locus_tag="PLF85_09330"
     CDS             complement(5909..6916)
                     /locus_tag="PLF85_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE19909.1"
                     /translation="MKRRISAVLRLLLTLVVSGVCLYFAARRIELDALRSEIGNLTWF
                     FSAPAVLASVAALSLRSLRWHLVLVREKNFAFSNTYWANGMGYLGNNILPARAGELIR
                     SVVLALSTGIRKSMVLATALTERMLDAGILLALAFIMLQFTEALPPSVQSIWSLMLPL
                     VLGLLAVAFAAPLLQVFWLRVLGALPMPEALRQKLRHLLLGLLDGIRVFHHLRLLIVF
                     LLLTCVIWVVDAAVFVFLARAFGAQLTLPQAVVFSAALGFASSIPSTPGFVGVFQAVA
                     VLVLPVFAVSANKAFLIVSLFQVMFLLITVLIGGYGWLVMRRRIGHAKLKEELQSESQ
                     T"
     gene            complement(6970..>7232)
                     /locus_tag="PLF85_09335"
     CDS             complement(6970..>7232)
                     /locus_tag="PLF85_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE19910.1"
                     /translation="HHALRCLKEARAKNVRLVEVSAEANSTYFADILARQQNTVFYNQ
                     DCSRSNSYYFDKHGDAPFYRPSSGIEMWLRARTFPLSDYRFT"
BASE COUNT         1589 a         2124 c         2023 g         1496 t
ORIGIN      
        1 ggcatgctgt acatgtcacg ggccctggga caacccctgc ccgaactgat ctcaaccgtc
       61 agttacgcga tgaacctgat tcgtatcgcg cacgccaagg aatacacggt attcctcttg
      121 ggttcacgcg acgaggtgct cgaaaaactc tacacgaatt ttcggcgatc gttcaccggt
      181 ctgcgcattg tagggcggca ccacggttac ctgaaaggcg aggccggaac acgggtactg
      241 gaggctgtgc gcaagacaga cccccatatt ctgttcgtag gcatgggcta ccaccgcggt
      301 atgaaatgga ttgagcagaa ccgcagccag ctgggcgatt tgattgtcgt caacgtgggt
      361 ggcgcattcg acacccttgc gggcacacgc cagaaggcgc ctgcgttcat ggcctctgcc
      421 ggatacacct ggttgtggcg cacgattaac aaaccctacc gctggcatcg tttcttcatg
      481 gtgatctact ggtttttcga agtcacctac taccgttttt caaagaagaa taaatgacct
      541 gagcagttag cgttccaggc cattattccg gtgtgggagc ttgtgagtcc tgtatgttct
      601 tgtaccagag acaggcttct ttcgtccccg cgataaggcc gagtgtttcc gtttctgcct
      661 tgttaaagac gagcggcgta taactgccat cgcgcagctc gagctgctct gccgcgagta
      721 atgcagcgcc gcccgcgaga tcccattcat tctttggttt taccgagacc acgaggtcaa
      781 acgttccgtc ggcgaccagc gccagcttgt acgcgatgga acccattgcc tgcttttcga
      841 gatcttcgta gtacttctgc aggtgcgtga tctggttctg tttaaattcg ctgcgcgata
      901 cgacaatacg aggataactt gctggcggcg caaatgcagg ccggtgcggc ggggtaaact
      961 ttttgccgtc acgaaagagc cccaggcacg ggccgccgct taccaggtaa tcggcagccg
     1021 ggttacagat aaacccgagc acggccctgc ccgcttcgac cagtcccact gaaacggcaa
     1081 actgtggccg tcccttgatg aattcacgcg tgccatcgat gggatcaata atccaaacac
     1141 gtttgcgctc tgaacgctcg gcattcatgg cgccgcgctc gtgcgggtgt tcaatttctt
     1201 cgctcagcag cgcatcgtcc gggaaaagat cccggattgc acgcacgagt attctattcg
     1261 cggcgaagtc tgcttcggtc acggggttct cgggatcttt atgctgcact tcgtacccac
     1321 cgcgggtgaa aaagcgcatc acggcgtgac cggcctcgcg cacgacaggt aacaaggcgt
     1381 tgtgttctgc tgaatagtct ggcatttaat tgaactccat ttcgagcgca ggattcagca
     1441 ggtgctgtgg atcaaacgcg ttcttgatac ttacctgcgc tgcgaaatat tcctttggca
     1501 aaatctggcg ggcgatgccg cgcttcagtc ggccaagacc gtgttcgccc gacatcgctc
     1561 ccccgagccg ggtgacttct gcagcaagtt cacgcataag ccccagcaca acgccgagcc
     1621 tttcgccgct ttgcagcagg tagttcacgt gcaggttgcc gtcacccgca tgcccccaga
     1681 tggctgtttc cacctgctgc gcggccgcct gtacgcgggc aaactccata gcgcggggca
     1741 aggccgaaac cggtacggcg acatcttcac cgatacggtg cggcgccagc ttcgaaactt
     1801 cgaggctcac agccttgcgt cggttccaca gggcctgccg gcttgtttca tcacgcgccg
     1861 cgacaaactc gtggctctcg gccagctgac caagacgccc gaggtcgtaa gcaacctctt
     1921 cggcggtgcc atcgagctcc accaacacga gcgcgccgcc cttaggacaa ccggggggaa
     1981 aatcaaccag tgaactgctg cggtagtcga gtatctcgat ggctgccgga cgaatggacg
     2041 accgcctgag tttcagaata aactccgctg ccgcgatttc gctggcaaag gctgccacga
     2101 gcaaagccga ttcttcaggc tgcggcagca ggcgaaactc ggcttcggtg ataataccga
     2161 gcctgccttc actgccacag aacaggtcgg gcagagaaaa gcccatagag gttttcaccg
     2221 gcagggcgcc ggcgcggaca aaatctcccg tcggggtgag aatcgttacc gccgcgaggt
     2281 tactgcgcgt tgtaccatac ttgagagacg acggccccgc agcattcgtc gcgacactgc
     2341 cgccgacggt gcagagagtt gaagatgacg gatcaacggc gtaacgtaag cccgtagccg
     2401 cgagcgcggt attcagctca gaaagtatga cgccgcactc tgcggtgatt gactggttct
     2461 gttcgtcgat acgaatgatg cggttcatgc gcgtgaggtc gagcacgaca ccaccctcgg
     2521 gaacacaggc accggaaagc ccgctgccgc cgccccgcag cgtcagggag agtgcacgac
     2581 tgtattgcgg cagagctttg cgcaacacct gcgcttcgat aacaatagca cggagttctt
     2641 ctgcattctg tggctgcagc accagcgcag cctgcccctt cagaaacgtc gcatctttca
     2701 ggtattgcgg aaattcttcg ctgtctgacg gccagacccg ccggccttgc gcgacaagcg
     2761 ctgcgaggct atcccacgcg ttctgcaacc tgtcgtcggg cgcggggccg ctattcatat
     2821 tgtcaggctg tggcacagag cagatgtgca aagtaaaagt tctggaaacc taatgcgcgc
     2881 atggcacaaa gcagcaatga ttgctatcct ggtttcgggc tgcatgtcga tcgaccgcaa
     2941 gattttcgaa gcaggctgtg ctcccccgcg ccggactgaa cgcgaaattg cgatcggcta
     3001 tgcactgccg ctgcaaaagc accattacct cggcgaaatc accatacagt atggctcggg
     3061 ctacgaacgc gacgagattt tacgcaggct gcgcgtggaa gccgcgcaat gcggtgcaga
     3121 cggtcttttg ctcgggtctt tttcccgcgt cgacagcaag tggaaatggg ccgacaagaa
     3181 cgtcaacgac tcgttcgaca cctctggcta caggctgatc gtcacgttgt atcgctacga
     3241 cgacagtgag aaagtcgccg aaccacccgc agcaaaacgg tagttagcca gctgttgcgt
     3301 tcttggcctg ctgccacagg ctatcccact cttcagtgga taaatgttca ccggcagcga
     3361 ctttttcccc ggctgtgagt tccatatgcc gaaagcggtt ttcaaatttc acgcaggctt
     3421 tgcgcagcgc cccttcagcg tcgatgccct gtcgccgtga atactgggcg agggcgaata
     3481 ataaatcacc cagttcttct tcacgccgct gcctgctttc gctttccgct gcttcaaact
     3541 cgcgaatttc ttcgtgaatt ttttcccaga gctgccggtc attaacccag tcaaaacccg
     3601 ttctcgcggc cttttcgccc agtcgttcgg ctctctgcag tgccggcaga attttgggta
     3661 cgccatcgag atatgatgtc cgcttctgct tacgcgcccg ttcgcgggcc ttgattgctt
     3721 cccagttttt cagtacctgc gccgaatcgg ccacctgcag ctcaccgtaa acgtgcgggt
     3781 ggcgtgtgat cagcttgtcg gcaatcgtgc gggcaacgtc ggcaaaagca aacctgccct
     3841 cttctgccgc gagctgggcg tgtataacga cctggaagag cacatcgccc agttcttctt
     3901 taaaagcctc gtcggtatcg ggggttaagc cgtgttcgat cacctcaatg agctcgcttg
     3961 cttcttcgag taagtgcttg gcgagactct cgtggctctg ttcgagatcc cacgggcaac
     4021 cgccgttctt gtcgcgcagg tcacgcgcaa cctgcagcag gtctgccagc gggtcagtgg
     4081 attgcggcgg cttcttcatg aggtcatttt tctgtggtgc gctgcgggcc acacaagaaa
     4141 atgacccgat gcgttcgctc gtaacgccct acctgctgac actgctgagc gactggttgc
     4201 ttatttccct ggcgtatgcc tggtattttc acgcgacggg ccaaaacctg ctgatcgtac
     4261 cggttttcag cggcgtgcag gttgccgtac tggcatgggc gaaactctac cgcgccaacc
     4321 tcaggtacct gaacctcttc tcactgcgca cgcttgcgat cgttgcgttt cttggcagcg
     4381 cggcgacgca acttgtctcg ttgccagcgg gaaggtattt tgttctcgag gcggtgatag
     4441 cctggttcgg gcaaaacacg ctgacactct tccaccggtt gttctggtat tttcgcctga
     4501 atcctgaagc cagactcaag aagcagggcc tcaccccgat agtcatctac ggcgcgggta
     4561 cggtggccta ccacctgctg caggatctct cggccgaaga cctcctgggc aaataccaga
     4621 ttgctgccgt cgtcgacaac gacccacaga aagagggagc gcatctcggg ccctaccgcg
     4681 tccacgcagg aaaacacctg ccaacccttg cggagaaata ccaggtgaaa gaaatctggt
     4741 tcaccatgcc tgttactcct gattttctca agcaggtgat ggaccaattg accggcatga
     4801 gcatcgccta caaagtgata ccccgcaagc tggcgcaatt tgccccggat atacgtaacc
     4861 tgcgtatcga agacctgata caacggcccg agataaggct tgatcccggc ccggtggagc
     4921 gcgcaatcag agggcgccga gtgctcgtga ccggcgctgc cggttctatc gggcgcgaac
     4981 tttcacggca gatcgcggca ctcgggcctg cacgccttac gctcatcgac cagtgggaac
     5041 acggcgtcta ccagctcgag caggaattca acggggaaaa tatcttctgc gcgatcgccg
     5101 atgtacgtaa tgagaagcgc ctcgcagaca tcatcacgca ggaaaagccg gaactcatct
     5161 ttcacgcggc ggcctacaaa catgtgccgc taatggaaac aaaccacctg caggcgatcg
     5221 acaccaacat catgggtacc tggcacctgc tggcggctgc gctgcgttac gctgcaggcc
     5281 tgccgcaggg tcagcaggtt cgaatggtca atatctccac cgacaaagcg gtgcagcctg
     5341 aaaacatcat gggcatcagc aagcgcatgg cggagctgct ggtttttaat gcggccagag
     5401 aatcagacgg gcggctgaag acgaccagcg ttcgctttgg taatgtactc ggctcttcgg
     5461 gttcggtcgt gccgctgttc tgggaccaga tacagcgcgg cggaccagtc accgtcacac
     5521 accccgagat ggaacgtttc ttcatgacaa tacctgaagc ggtgcgcctg gtcttgcagg
     5581 ccgcggcgct cgaagaagaa accgactgta ttttggcgct cgacatgggt aagccggtac
     5641 gcatcaccga acttgccgaa cgactgatac tgctcgcagg taaaaaaccc ggcagcgaaa
     5701 ttgcgattac ctactcgggc atacggccgg gcgaaaaact caaagaagaa cttttctggg
     5761 agaccaattc ggtacgcaca gcaatcccct ttatttttcg ctcaaccgcc gacctgaaag
     5821 aaatggatat acagggcttc atgggattat tgcagagcgc gctcgaagaa aatcgttcac
     5881 gctcgtggta ccgcgaattt ttgctgcgtt acgtctgact ttctgactgc agctcttctt
     5941 ttaattttgc atggccgata cgccgccgca tcaccagcca gccataaccg ccgataagca
     6001 ctgtgatcaa caggaacatc acctggaata aagaaacaat gaggaatgcc ttgttggcgc
     6061 tcacggcaaa tactggcagc accaggactg ccacggcctg gaatacaccg acaaaccccg
     6121 gggttgaagg tatgctgctg gcaaacccca gcgctgcaga aaaaaccact gcctgcggca
     6181 gcgtaagctg ggcgccaaat gcccgggcaa gaaagacgaa cacggcagca tcgaccaccc
     6241 aaatcacgca ggtgagcaga agaaaaacaa tgagcaggcg caggtggtga aagacccgaa
     6301 taccatccag cagtccgagt aacaggtggc gcagcttctg cctgagagcc tctggcattg
     6361 gtaaagcgcc gagtactctg agccagaaca cctgcagcag tggcgcggca aacgccacag
     6421 caagcaaccc cagcacaagc ggcagcatca gcgaccagat actctgcacc gacggtggca
     6481 aagcttctgt gaactgcagc atgatgaagg ccagcgcgag tagtataccg gcgtcgagca
     6541 tccgttcggt cagggcagtt gccagcacca tcgacttacg aatgccggtg ctgagggcca
     6601 gcacgacaga acgtatgagc tcccccgcgc gtgccggcag gatattattg cccaggtagc
     6661 ccatgccgtt tgcccaatag gtattgctga aggcgaaatt tttttcgcgc acgagtacca
     6721 ggtgccagcg caggctgcgc aggctcagcg cggcaacgga ggccagcact gcaggagcgc
     6781 tgaagaacca agtgagattg ccgatctcag aacgcagggc atcgagttcg attctgcgcg
     6841 cagcaaagta aaggcagacg cccgagacaa ccagcgtcag caacaggcgc agtactgcag
     6901 atatccgccg cttcattgcc ccagttaccg aaatggacct gcggtgtcgt gcctgaatta
     6961 cccgtaacgt caggtgaacc ggtagtccga cagcggaaat gtacgggcgc gcagccacat
     7021 ctcgatgccc gaagaaggcc ggtagaaagg cgcatcaccg tgcttgtcga aatagtagct
     7081 gttagagcgg ctgcagtcct ggttgtagaa caccgtgttc tgctggcggg caagaatatc
     7141 ggcaaaatat gtggaattgg cttctgcaga gacctcaacc aaccgcacgt tcttcgcccg
     7201 cgcctctttc agacagcgca gcgcatggtg ca
//
LOCUS       DAOQJF010000156         7219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5722_length_7219_cov_5.618649, whole genome shotgun sequence.
ACCESSION   DAOQJF010000156 DAOQJF010000000
VERSION     DAOQJF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5722_length_7219_cov_5.618649"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLF85_09340"
     CDS             <1..238
                     /locus_tag="PLF85_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19911.1"
                     /translation="RRNAERVSAERIRYLYFAVLAALGLITTFSLLTFRQVSRQRRPH
                     APMNDFPAAVPVPQTAPARSQVKRKPAKKKSRGK"
     gene            243..1127
                     /gene="rfbA"
                     /locus_tag="PLF85_09345"
     CDS             243..1127
                     /gene="rfbA"
                     /locus_tag="PLF85_09345"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNE19912.1"
                     /translation="MKGIILAGGSGTRLYPVTRVVSKQLMPIYDKPMIYYPLSALMLA
                     GIREILIISTPSDLPRFRELLGTGEDLGMQFSYAEQPSPDGLAQAFIIGADFIGKDSV
                     ALVLGDNIFHGHDLTELVMRAVERTEKQGGAEVFAYPVHDPERYGVVEFDGKFNALSI
                     EEKPAQPKSNYAVVGLYFYDNGVVAKAKNLKPSARGELEITDLNRIYLHEQKLRVSVM
                     GRGFAWLDTGTHDSLLDASHFVSLIEKRQGLKVACIEEIAYYKKYISAEQLIKLAKPL
                     EKIEYGKYLLRLAKQPVP"
     gene            1124..1666
                     /gene="rfbC"
                     /locus_tag="PLF85_09350"
     CDS             1124..1666
                     /gene="rfbC"
                     /locus_tag="PLF85_09350"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNE19913.1"
                     /translation="MSLKYPKPILVEPRVFGDARGYFFEPYNRNTFLAQWGIDDSFVQ
                     DNESYSNRGVLRGLHYQAGDKAQAKLVRVVTGSVLDVVVDIRKSSPYFGQVYSYVLED
                     TKKHMLFVPRGFAHGFAVLSETAIFQYKVDNFYAKDSERGIHYADKRLAIDWQLPADS
                     ILVSDKDKVLPAFENAEYFD"
     gene            1669..2562
                     /gene="rfbD"
                     /locus_tag="PLF85_09355"
     CDS             1669..2562
                     /gene="rfbD"
                     /locus_tag="PLF85_09355"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNE19914.1"
                     /translation="MADTLRILVTGGKGQLAQCLRKQQPEAYAVTYAPRAALNLEDHA
                     STRAQITGMKPDVVINAAAYTRVDQAEKEPEAAFAANELAVKNLAEVCRDTGALLLHI
                     STDFLFHGAFNRPIDEQQAPAPIGIYAQSKLAGEKAVLASGVSGAIVRTSWLYSEFGN
                     NFMKTMIRLAGEKPRLTVIADQHGSPTYAGDLAAALWQMTEKLAERKLARCQTWHFSN
                     YGVTTWYDFAVNILVLAGSKTPVEPILTAEYPVPTPRPAYSALWPRGFSQEFDFPIRN
                     WRLALADAVAAYNKQQDGAFL"
     gene            2559..3584
                     /gene="rfbB"
                     /locus_tag="PLF85_09360"
     CDS             2559..3584
                     /gene="rfbB"
                     /locus_tag="PLF85_09360"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNE19915.1"
                     /translation="MKTIIVTGGAGFIGSNFILHMLERYQDYKILNLDKLTYAANKEY
                     LESVASDPRYELVVGDILDRELVMRLFSQHDVAGVVHFAAESHVDNSISGPAVFIETN
                     IKGTLTLLEAARAHWMDAPFRPKAGKEHCRFLHVSTDEVYGSLGETGLFREDTPYAPN
                     SPYSASKAGSDFLARAWHHTYGMNTVITNCSNNYGPHQHAEKLIPTIIRKALAGEKIP
                     IYGDGKNVRDWLYVRDHCSGIDLAFHQGKAGETYNIGGRNERNNLQIVGTITELLQQL
                     APPPSRQLSIAHYKDLITFVADRPGHDRRYAIDATKIETQLGWKAQENFESGIRKTVE
                     WYLAKGR"
     gene            3678..4991
                     /locus_tag="PLF85_09365"
     CDS             3678..4991
                     /locus_tag="PLF85_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNE19916.1"
                     /translation="MNHVLLELIFIPILIFLNGIFSLAEMAVVSSRRSRLQVLADAGS
                     RGARQVLKMLDDPERFFSTIQIAITLISILTGVISGAAATDVLAAYLKERFPSYAQHA
                     VSVAFFSIVFFATYLAIVFGELIPKSIAVRHSETIAVLVAYPLRLVIGFFSPFSYVLS
                     ATAKIILRPFGIRGNNKAQVTEEEIRIMLQEGHETGAVLSSEKSMVENVFRLDDIAVV
                     TVMTPKPLMTWLDVANPIQDILETIREHNHSYYPVVDGSLEKVIGVLPARPLLADLNL
                     QQARNLKDYLVSPLYVPESMKASRLLETFKQTQRHFALITDEFGGIQGVATLHDILEA
                     IVGDLPPQSGDTEKKFRRRDDGSVAITGTVTLAEFRDELGFSLVEGLEDDQPYSTVAG
                     FLAAHMQRIPTEGERIAIPEGGFEIVDMDGPRIDKILFFPSKSAQ"
     gene            complement(4975..6384)
                     /gene="murF"
                     /locus_tag="PLF85_09370"
     CDS             complement(4975..6384)
                     /gene="murF"
                     /locus_tag="PLF85_09370"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNE19917.1"
                     /translation="MFARIDYDFFTVASICSTHTPAVQSPEIRLRSITTSSLQVEPGS
                     LFVPLTDRRDGHEFIGDAVRRGAAAFLVRKGHEVLKTLDEKTRALAIVVDEPLLALGR
                     LAAFHRNRFSPLVIAVTGSNGKTTTKEMIGQIFRTALGRRALYTEKNYNNHIGVPFTL
                     FRIGKDTRVAVIEMGMNHAGEIDYLSRMARPHVSVISSIGHAHIEFLGSRQNIAQAKA
                     EITHGQQKGGALYVPAQVAELATLSARSRANGIVLKKIQPGKSAPLKVTTVAPIGYEL
                     LIGKTPVVFRHANNAWLSNLALAAAVAHDAGISEQLIAKAVSSFRPPDGRMQVSKGRF
                     HVIDDGYNANPDSAIASIAAAKQYADGRPVICVFGDFKELGKFSRSLHEWTGKEAAKA
                     RVGAFYGIGRDMRLAAKAYARAAGKSGRSYAFERGDIPAIVAQLVQEAKGSVILVKGS
                     RSMQMEEIVQSLQAAIARI"
     gene            6458..7204
                     /locus_tag="PLF85_09375"
     CDS             6458..7204
                     /locus_tag="PLF85_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE19918.1"
                     /translation="MHILLAGYGFVGSGVAELLSAAGHQLTVLRRRQTGQKIGIEFVT
                     CDLAYDRPWSTKRQFDTVVFSLAPDERSDAAYTATYCTAQENLQSAIETQHYVYVSST
                     AVYPETAGTWREEDAAGHSERARVLLEAEAIADRMPACAILRFAGLYNRDRRIYYGPP
                     ATVSDDRLVHFLHHADAARAISHAITQKLRGVFNVHDGNPQWRSDILCRLGFLNTALP
                     RGTERRISAEKFFSTGFRPSYPDFFSGLAF"
BASE COUNT         1665 a         2057 c         1935 g         1562 t
ORIGIN      
        1 ccgccgcaac gccgaacgcg tatcagcgga gcgcattcgt tacctctact tcgccgtact
       61 cgccgctctc ggccttatta ctaccttcag tcttttgact ttccggcagg tgagccggca
      121 gcgccgacct cacgcaccga tgaacgattt tccggcagcc gtaccggtac cgcaaacggc
      181 gccggcaaga tcgcaggtta aacgtaagcc agcgaaaaag aaatcgaggg gaaaataatt
      241 ccatgaaagg cataatacta gcgggcggtt caggtaccag gctctacccg gtgacacggg
      301 tagtctcgaa gcagctgatg ccgatctacg acaagccgat gatatattac ccgctctcgg
      361 cgctgatgct cgcgggcata cgcgagatac tgatcatttc gacccccagc gatttaccgc
      421 gctttcgcga gctgctcggc acaggcgaag atctgggtat gcagttcagc tacgccgagc
      481 agccatcgcc cgatggcctg gcgcaggcat tcatcattgg cgccgacttc atcggcaaag
      541 attccgtagc gcttgtgctc ggggacaata tctttcatgg tcatgacctg accgagctgg
      601 tgatgcgcgc tgtggagcgc acggaaaaac aaggcggggc ggaggtattt gcgtacccgg
      661 tgcacgatcc cgagcgttat ggcgtcgtcg aatttgacgg taaatttaac gccctctcta
      721 tcgaggaaaa gccagcacaa cccaagagca actacgcggt tgtcggactc tatttttatg
      781 acaacggtgt tgtagcaaaa gcaaaaaatc tcaaaccatc agcgcgcggc gagctggaga
      841 tcaccgacct caaccgcatc tacctgcacg aacagaagct gcgcgtcagc gtcatgggac
      901 gcggttttgc ctggctggac accggtacgc atgactcgct gctcgacgca tcgcacttcg
      961 tcagcctgat tgaaaaacgc cagggactga aagtggcctg cattgaagaa atagcctatt
     1021 acaaaaaata catctctgcc gaacagctca tcaaactcgc caagccgctg gagaaaattg
     1081 aatacggcaa atacctgctg cgcctggcga aacaacctgt accgtgagcc tgaaataccc
     1141 aaaacctatt ctggttgagc cacgcgtttt tggcgatgcc cggggttact tttttgagcc
     1201 ctacaatcgt aacacatttt tagctcagtg gggtatcgat gattctttcg tccaggacaa
     1261 tgagtcgtat tcgaaccgcg gcgtgttacg tggcctgcac taccaggctg gcgacaaggc
     1321 gcaggcaaaa ctcgtgaggg tagtgacagg ctcagtgctc gatgtagtcg tcgatatccg
     1381 caaaagctcc ccctactttg gccaggtcta cagctacgta ctcgaagata cgaagaagca
     1441 catgctgttt gtaccgcgcg gctttgcgca cggtttcgcc gtactttcag agactgcaat
     1501 tttccagtac aaggtcgata atttttacgc caaagatagc gagcgcggca ttcattatgc
     1561 cgacaagcgg ctggcgattg actggcaact gcctgcagac agcatactgg tttctgacaa
     1621 ggacaaagtg ttgccggcat ttgaaaacgc ggagtatttt gactgaccat ggctgacacc
     1681 ctgcgcatac tggtgaccgg cggcaagggc cagctggcgc aatgcctgcg caagcagcag
     1741 ccagaggcct acgcggtaac ctatgcccca cgggcagcgc tcaacctcga agaccacgct
     1801 tctacccggg cacagatcac cggtatgaaa ccggatgtgg tgattaatgc ggcagcctac
     1861 accagggtcg accaggcgga aaaagaaccc gaagcggctt ttgcggcaaa cgaacttgca
     1921 gtgaagaacc tcgctgaagt ctgccgcgat acaggcgcgc ttctgctgca tatttcgacc
     1981 gactttcttt tccatggagc cttcaaccgg ccgattgacg agcagcaggc accggccccg
     2041 atcggcattt acgcacagag caaactcgcc ggcgaaaagg ccgtattagc ttccggcgtt
     2101 tcaggagcga ttgtgcgcac gtcatggttg tattcagaat ttggaaacaa cttcatgaag
     2161 accatgataa ggctggcggg agaaaagccg cgcctgacgg tgattgccga ccagcacggt
     2221 tcaccgacct atgcgggtga cctcgccgcc gcgctctggc agatgaccga aaaacttgcc
     2281 gaacgcaagt tggcccgctg ccagacatgg catttcagta attacggcgt gactacctgg
     2341 tacgacttcg cggtcaatat actggttctt gccggcagca aaacaccggt tgagccgatt
     2401 ctcactgcag agtaccctgt accaacgcca cgcccggcat acagcgctct ctggccgcgc
     2461 ggcttttcac aggagtttga ttttccgatc aggaactggc gtctggcgct tgctgacgcc
     2521 gttgcggcat acaacaaaca acaagacgga gctttcttat gaaaactata atcgttaccg
     2581 gcggggcagg ctttatcggt tcgaacttta tattgcacat gctcgaacgc taccaggatt
     2641 acaaaattct gaatctcgac aagctgacct atgccgcgaa caaggaatac ctggaatccg
     2701 tcgcgtccga cccacgctac gaactggtag tcggtgacat actcgaccgc gaactcgtga
     2761 tgcgcctatt cagccagcac gacgttgccg gggtcgtgca cttcgccgca gaatcgcatg
     2821 tcgacaattc tatcagcggc cccgctgtct ttatagagac gaacatcaag ggcaccctga
     2881 ccctgcttga agcagcccgc gcacactgga tggacgcacc gttcagaccg aaggcgggca
     2941 aagagcattg ccggtttttg catgtcagta ccgacgaggt atacggttcg ctgggtgaaa
     3001 cgggcctttt ccgcgaagac actccctatg cgccgaacag cccttacagc gcctcaaagg
     3061 ccggcagtga tttcctggca cgcgcatggc accacacgta cggtatgaat accgtcatca
     3121 cgaattgctc caacaactac ggcccgcacc agcatgcgga aaagctgatc ccgacaatca
     3181 tacgcaaggc actcgccggc gaaaaaatcc ccatttacgg cgacggcaaa aatgtgcgcg
     3241 actggcttta tgtgcgcgac cactgttcag gcatcgacct cgcgtttcac cagggcaagg
     3301 caggtgagac ctacaacatc ggcgggcgca acgaacgcaa caacctgcag atcgtcggca
     3361 cgattaccga actattgcag cagctggcac caccaccatc acggcagctg agtattgcgc
     3421 actacaaaga cctgattacg tttgtcgctg accgcccggg tcacgaccgc cgttatgcta
     3481 tcgatgcgac caaaatcgag acgcagctgg gctggaaggc gcaggaaaac ttcgagagcg
     3541 gtatccgcaa aacggtggag tggtatctcg caaaaggcag gtagactgat cgccctgcgg
     3601 gcgcgtgcat tggttgaccg cgcagcgcag gttgcggtcg ttcaaccggg ggtatcgatg
     3661 ccaataccca aaccctaatg aatcacgttc tactggaact cattttcata cccatcctga
     3721 ttttcctgaa cgggattttc tccctcgctg aaatggcagt agtctcatcg cgtaggtcgc
     3781 ggctacaggt gcttgccgac gcgggtagcc ggggagctcg gcaagttttg aagatgctcg
     3841 atgacccgga gcgttttttt tctactatcc agatcgcgat tacgctgatt tctatattga
     3901 ccggcgtgat cagcggtgcg gccgcgaccg acgtactcgc cgcttacctg aaggaacggt
     3961 ttccgtctta cgcgcaacat gccgtttctg tcgctttctt cagtatagtt ttttttgcca
     4021 cctaccttgc tattgtattc ggtgaactga tcccgaagag tattgcggtc agacattccg
     4081 agactattgc ggtcctggtg gcttacccgc tgcggctggt cataggtttc ttttctccgt
     4141 tctcttacgt actgagcgcc acggcaaaaa taatcctgag accattcggt attcgcggca
     4201 ataacaaagc gcaggtaaca gaagaagaaa tacggatcat gctgcaggag ggacacgaaa
     4261 caggggccgt gctctcttca gaaaaaagca tggtcgaaaa cgtctttcgc ctggatgaca
     4321 ttgctgtagt taccgtcatg acacccaagc cattaatgac gtggcttgat gtggcaaacc
     4381 cgatccagga tatcctcgaa acgatccggg aacataacca ttcgtactat ccggtggtcg
     4441 atgggtcgct cgaaaaagta attggagtgc ttccggcacg accactgctc gccgacctga
     4501 acctgcaaca ggcccgaaac ctgaaagact accttgtttc acccctttac gtacctgagt
     4561 cgatgaaagc ttcgcgcctg ctcgaaacat tcaagcagac acagcggcat tttgccctga
     4621 tcaccgacga atttggcggc atccaggggg tcgcaaccct gcacgatatt cttgaggcga
     4681 tcgtgggcga cctgccgccg cagagcggcg ataccgaaaa aaagttccgg cgtcgcgacg
     4741 acggcagcgt tgccattaca ggcactgtta ctctggctga attccgcgat gagctcggct
     4801 ttagccttgt cgaaggatta gaggatgacc agccctattc taccgtagcc ggatttcttg
     4861 cagcacacat gcagcgcatt ccaaccgaag gagagcgtat cgcgataccc gaaggcgggt
     4921 ttgagattgt cgatatggat ggtccacgta tcgacaagat tctttttttc ccttctaaat
     4981 ccgcgcaata gctgcctgca gcgactgcac aatttcttcc atctgcatcg agcgtgaacc
     5041 tttcacgaga atgacactcc ctttggcctc ttgcacaagc tgcgcgacaa tcgcgggaat
     5101 atcaccccgc tcaaatgcat aactgcgtcc ggactttcct gcggccctgg cgtaagcctt
     5161 cgcagcaagc cgcatgtcgc ggccgatacc gtagaacgcc ccaacgcgcg ccttcgccgc
     5221 ttctttgccg gtccattcat gcagcgagcg gctgaatttt ccgagttcct tgaaatcgcc
     5281 aaacacgcaa atgaccggac gaccatcggc gtactgtttg gcagctgcaa tagaggcgat
     5341 tgccgagtca ggattcgcat tgtatccatc gtcgatgacg tggaagcgac ccttgctcac
     5401 ctgcattcga ccgtcgggtg gacggaacga gctgacggcc ttagcaatca gctgctcaga
     5461 tatgcccgca tcgtgagcca cggctgccgc aagcgcgagg ttggaaagcc aggcattgtt
     5521 ggcgtggcgg aaaaccacgg gagttttgcc aattaacagt tcgtatccga ttggagccac
     5581 ggtagttact tttaacggag ccgatttacc cggctgaatt tttttcaata caataccgtt
     5641 ggcgcgcgaa cgggcagaga gagtcgcaag ctcggcaacc tgtgcgggca cataaagcgc
     5701 tccacctttt tgctggccgt gcgtgatttc agccttggcc tgcgcgatat tctgccgcga
     5761 accaaggaac tcaatgtgcg catgcccaat actgctaatg acggacacat gcgggcgcgc
     5821 catgcgcgag aggtaatcga tctcacctgc atgattcatt cccatctcga taacagccac
     5881 tcgcgtatct ttgccaattc ggaagagcgt gaacggcaca ccgatatggt tgttgtagtt
     5941 tttttccgta tagagagccc gacgaccgag ggccgtgcgg aagatctggc caatcatctc
     6001 tttcgttgtt gttttgccgt tagagcccgt aaccgcgatc accagaggcg aaaagcggtt
     6061 gcggtggaaa gcagccagtc tgcccagcgc cagcagcggc tcatcaacga cgatagccag
     6121 cgccctcgtt ttttcatcga gagtcttgag cacttcatga cctttgcgca ccaggaatgc
     6181 cgcggccccc cggcgtaccg catcgccgat gaattcgtgt ccatcgcgcc ggtcagtgag
     6241 cggtacaaaa agcgaacccg gttcaacctg caacgacgag gtggtaatcg agcgcagacg
     6301 gatctcaggc gactggactg ctggggtatg cgtgctacag attgaggcga ccgtgaagaa
     6361 gtcgtagtca atacgcgcga acatgtgtcg aaaaaggtct gggtaatgca gcgtcacttt
     6421 cttctcgctg gcctgtgcag attcgctgtc gtgccacgtg catattctgc tcgcgggtta
     6481 tggtttcgtc gggtctggcg ttgctgagct gctctccgct gccggccacc agctgaccgt
     6541 gttgcggcgg cggcagaccg ggcagaagat cggcatagaa tttgttacat gcgacttagc
     6601 ttatgaccgt ccatggagca cgaagcgcca gttcgatacc gtggtgtttt cgcttgcccc
     6661 cgatgagcgc agcgacgcag cctatactgc aacgtactgt actgcgcagg aaaacctgca
     6721 atcggcgatt gagacacaac actacgttta cgtctcgagc actgcagtct accctgagac
     6781 tgccggtacc tggcgcgaag aagacgctgc ggggcactct gagcgggcgc gcgtactgct
     6841 tgaagcagag gcaattgcag accgtatgcc tgcctgcgca atactccgtt ttgccggact
     6901 atacaaccgt gaccgcagaa tctattacgg tccgcccgcc accgtctctg atgaccggct
     6961 ggtccatttt ctgcaccatg ccgatgcggc gagggctatc agccacgcga ttacgcagaa
     7021 acttcgggga gttttcaacg tgcacgatgg taatccacag tggcgatctg acatcctatg
     7081 ccgcctggga tttctcaaca ctgccctgcc gcgcggaacc gaaagacgca tctctgcaga
     7141 gaaattcttt tccacaggtt ttcgaccatc ttatccggat tttttctccg gcctggcatt
     7201 ctgacgatct accccccat
//
LOCUS       DAOQJF010000157         7196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5750_length_7196_cov_4.060496, whole genome shotgun sequence.
ACCESSION   DAOQJF010000157 DAOQJF010000000
VERSION     DAOQJF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5750_length_7196_cov_4.060496"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLF85_09380"
     CDS             complement(<1..514)
                     /locus_tag="PLF85_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19919.1"
                     /translation="MARLLPLQQEPWYMRRVKQSIINALKEWVLLPPKQTPLKLQRHF
                     ISSNTIFVLAFGIHFAFIFFFLVFGVPEMMWFNIASCAWFVGTIYLNRKLYLFTALHL
                     CFFEVLAHAMACVHFFGWESGYQYYAILLPTGIFLLPPGKTWLKFFTIITGCAIFAGG
                     FYYSLSHPPVY"
     gene            complement(549..761)
                     /locus_tag="PLF85_09385"
     CDS             complement(549..761)
                     /locus_tag="PLF85_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNE19920.1"
                     /translation="MILCSCANVNDRKVREALQSGARTLADVQQELGVAMGCGRCREA
                     VCEEISRHCGAISADATYKTALPQAV"
     gene            895..2334
                     /locus_tag="PLF85_09390"
     CDS             895..2334
                     /locus_tag="PLF85_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19921.1"
                     /translation="MRPIAHLALFTLLPASLFAATFRDAVRSMGPGNTGVTLARELVT
                     VANLMSEHLEYDENSRRFFAFMNDALEDFDFSRIYDSPWLTPQADGFRSIALSGRNTG
                     STYRVSLGREGNASRQLGSATAEYRMRENRIAGDKTFTTQLAFDAALQKFDAQAATRF
                     AEGMLKVFDPASIAQLRPPASAAFPEIQGDERRIIDLGMAEFPRFTQMLSRYIELKSF
                     ANVRDYKGKKYTEVAMRGRFRLDALGADFPRLKSFIKDLRKLFVLSVYIGDTKGRQIS
                     SFIVNMQTEEFYAGFNTLDGKILPMGKDGAPAFDAPVSFSGNADHKFYLAFNFFVNVH
                     GLKINTGNVGAYLRYQANAERMSFYTKLTQMPEGKISGALFGVLPTWLIDLSIPSDLQ
                     TLMNKFSQTIYKANNGEGSFAQIAWKKQSGQAAFHANASTEFLENRFIRIGMKIWVKK
                     FRPNEQVQDDIRRFIGSFTRALLADLNAM"
     gene            complement(2308..3402)
                     /locus_tag="PLF85_09395"
     CDS             complement(2308..3402)
                     /locus_tag="PLF85_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNE19922.1"
                     /translation="MPTGKRMAKHRNPVRKEMLHNRLSREQLLKQIAAEPPVPRRTLS
                     FYRYLKIENPRDLRDELYREWSELGVRGRIYLAREGINAQLSVPLANLDGFRAALEGR
                     PGFGSMRFNPGVEDDGNSFLKLTIKVKRYIVNDGLGDFELDASVAAPHLKPLEFNRAL
                     EEPGTVVVDMRNQYESEVGHFEGAICPDVPTFREELPIVADMLKDKKDEKILIYCTGG
                     IRCEKAGAYLRKQGYRNVYQLEGGVINYAHAVKQEGLPSKFKGKNFVFDERLGERITD
                     DVLAKCHICGASSDRQINCANEGCHVLMVQCEACAEKLNNCCSEECREISLLPPEKRR
                     ELRKADPNLKNKAWFSKKIRPRSHRVEVGE"
     gene            complement(3472..4395)
                     /locus_tag="PLF85_09400"
     CDS             complement(3472..4395)
                     /locus_tag="PLF85_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNE19923.1"
                     /translation="MNRLTQLFPLIAAAASALALFYPPIFTWFGPLYIKVALALIMFF
                     TGLSLTTADFKRVARKPGQVAAGFVLQYTVMPAMGYFTALAFALPAEIAAGLILVACC
                     PGGTASNVITFLAKADVPLSVTMTAVSTIAAAMFTPALSAWLIGARIPVDALAMTRDT
                     AMVILLPIALALVAKRVLPHAAKKLEHSANFLAVLAITLIVASIIGSSRAQIISGGFS
                     IIAAVFTLHAGGFLFGYVFSRLLRIPETQARTISIEVGMQNSGLGVVLAMKHLTPLAA
                     VPCAVSSLMHSLIGSLLAGLFSRRPAAAREP"
     gene            complement(4445..5587)
                     /locus_tag="PLF85_09405"
     CDS             complement(4445..5587)
                     /locus_tag="PLF85_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNE19924.1"
                     /translation="MANEHADYKPGLDGVIACITKLSLLDEEKEQIIVRGYDLIELIQ
                     KTSYIKVAYLLIYNKFPTDAEAKAFEQKLMAQADIPEEMYEIFKLLPKKTMAMDALRT
                     GISILGGYEDHAALMDTSTKSNEEKALKILAKAPAIAVNSYRAIMGLPFVKPDKSLGY
                     SENFVYMIKGSKPTAVETDTLDKILTTYIEHEMPNSTFAVRVVSSTLSDIYGSCASAV
                     ASLKGPLHGGANEAAMQMFLEVQKTGGPSTAESYIMGKLQRKDKIMGFGHRVYMKKYD
                     PRAFLMKDYIPKLVDQRPDGKDLYSIYQTIEKTMAREKGLYPNADYPIALLYYLINVP
                     IDLYTPIFLCSRLAGLVSHHIEQHENNRLFRPRVIYEGARDLHPPQ"
     gene            5735..6784
                     /locus_tag="PLF85_09410"
     CDS             5735..6784
                     /locus_tag="PLF85_09410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE19925.1"
                     /translation="MAAERVLLLQDWYDENDLLNPSFFALLSQFPDAHVGFLKNFSTT
                     LPEALAQRRTEFYAARLPRKGFDNLEALIKIQSEIPIADYDLVICNTRGYLRHLRREK
                     DAHTRIIVYQHDLLPFLWRADTERLNTQKAAELLRLQESDLEFAAGIDVIVAANFALK
                     TTLAAMHRRTVPLAYPLVDSDLFFPDSEAEAEYFVATDSADLEALLHLFACVNDKLVV
                     LGDYRQNKLFRELKPDNIFYAGNLPATEKAYYLAGAKAVFCGETRTLDHLPLAALKSG
                     IPVIAHPTQGMHEFLVDGDLGAELETGSADELIRYIRFYRERMTDRAKIAGEVDWLNR
                     EYFLRRWRKVLEHGQ"
     gene            6781..>7196
                     /locus_tag="PLF85_09415"
     CDS             6781..>7196
                     /locus_tag="PLF85_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19926.1"
                     /translation="MKARRFIIFSLAATALSGAPEEKKLHRILFSGLTRTHENVVRQF
                     IAVKPGEPITAADAEELRTQILRLRIFSEARVELLERDSAEPCRALCDLHITVREKWS
                     LYPVPVYVRYRDTEIGGAFAVESNFLGQNKGLAVGAL"
BASE COUNT         1586 a         2051 c         1979 g         1580 t
ORIGIN      
        1 tgtagacagg cgggtgcgag agtgagtagt agaacccccc ggcaaaaatt gcacagcccg
       61 tgatgatcgt aaaaaacttg agccaggttt tgcccggcgg cagaagaaag attcccgtcg
      121 gcaagaggat agcgtagtac tggtaacctg attcccaccc gaagaagtgc acacaggcca
      181 tcgcgtgcgc aagtacttcg aagaaacaaa gatgcagcgc cgtgaagagg tagagcttcc
      241 ggtttaggta gattgtcccc acgaaccagg cgcagctcgc gatattgaac cacatcatct
      301 cgggcacgcc gaagaccagg aaaaagaaga tgaacgcaaa atgaatgccg aacgcaagaa
      361 caaagatcgt attactcgag ataaaatgcc gctgtagctt gagcggggtc tgcttcggcg
      421 gcagtaacac ccactctttc aatgcattga tgatagattg cttaactcgt cgcatatacc
      481 agggctcctg ctgcagcggc aaaagccgtg ccatcaggga ctctgtcaat gcttagtaga
      541 tgcgttgctc agacagcctg cggcagagct gtcttatagg tggcatctgc gctgatagcg
      601 ccgcagtggc ggctgatctc ttcgcagact gcttcacggc atcggccgca gcccattgcc
      661 acgccgagtt cttgttgcac atcagccagg gtacgggcac cgctctgcag cgcttcgcgc
      721 accttgcgat cgttgacgtt cgcgcaggaa cacaggatca tgcgttactg agaataattc
      781 tcactcgccg tcaactcgtt tctgcgtttt cataggggct tcggcagcac tttaccggct
      841 taccgggcag cattttgcta tgcactcgcg gcagattgta caattggcaa gcccatgcgc
      901 ccgatcgccc acctcgcact ttttaccctg ctgcccgcca gtctttttgc cgctaccttc
      961 cgtgacgcgg tgcgctcgat ggggcctggc aacaccggcg taacgctggc ccgcgaactt
     1021 gtgaccgtag ccaacctgat gagcgagcac ctcgagtacg acgagaattc ccgccgcttc
     1081 tttgccttca tgaacgatgc gctcgaagac tttgacttca gccgtatcta tgatagccca
     1141 tggctgacac cccaggccga cggctttcgc agtattgcgc tgagcgggcg taataccggt
     1201 agcacctacc gggtctcgct ggggcgcgag ggcaatgctt cacgccagct cggcagcgcg
     1261 actgccgaat accggatgcg cgaaaaccgc atcgccggtg acaaaacttt caccacgcag
     1321 ctcgccttcg atgcagcgct gcagaagttc gacgcgcagg cggctacgcg cttcgccgag
     1381 ggcatgctca aggtctttga cccggcttca attgcgcagc tgcgcccgcc tgcgtctgca
     1441 gctttcccgg aaatccaggg cgacgaacgg cgcattatcg acctgggtat ggcggagttt
     1501 ccacgtttta cgcagatgct ctcgcgctat atagagctca aaagttttgc caacgtacgc
     1561 gactataaag gtaagaaata tactgaagtg gcgatgcggg gccgctttcg cctcgatgcg
     1621 ctgggggcag attttccccg gctgaaatcg ttcatcaaag acctgcgcaa actttttgtc
     1681 ctttcggtct atatcggcga caccaagggc aggcagattt catctttcat cgtaaacatg
     1741 cagactgaag aattttacgc gggcttcaat accctcgacg gcaagattct gcccatgggc
     1801 aaagacggcg ccccggcatt tgatgcaccg gtatccttca gcggcaatgc cgaccataag
     1861 ttctacctcg ccttcaactt tttcgtcaac gtgcacggcc tgaaaattaa cacaggcaat
     1921 gtgggagctt acctgcgtta ccaggccaat gccgaacgca tgtcttttta tacgaaactg
     1981 acgcaaatgc ccgagggaaa gatctccggt gcactgttcg gcgtgctgcc gacctggctg
     2041 attgacctgt ctattccgtc agacctgcag acgctcatga acaagttttc gcagacaatc
     2101 tacaaggcaa acaacggcga aggctcgttt gcgcagatcg cgtggaaaaa acagtcaggc
     2161 caggcggcgt ttcatgccaa cgcatcgacc gaatttctcg aaaaccggtt tatccggatc
     2221 ggcatgaaaa tctgggtaaa aaaattcagg cccaatgagc aggtgcagga cgatatccgc
     2281 cgcttcatcg gctcgttcac gcgcgcgcta ctcgccgacc tcaacgcgat gtgatcgtgg
     2341 gcgaatcttc ttgctgaacc aggccttgtt tttcaggttc gggtcggcct tgcgcagctc
     2401 gcgccgcttt tcgggcggca gcaggctgat ctcgcggcac tcttcgctgc agcaattgtt
     2461 tagcttttcg gcgcaggcct cgcactgcac catcagcacg tggcaaccct cattcgcaca
     2521 gttgatctgc cggtcgctgc ttgctccgca gatatggcat tttgccagca cgtcgtctgt
     2581 gatccgctcg cccaggcgtt cgtcgaaaac aaaattcttg cccttgaatt ttgaaggcag
     2641 cccctcttgc tttaccgcgt gcgcgtagtt gattacgccg ccttcgagtt ggtacacatt
     2701 cctgtaaccc tgctttctca ggtaagctcc ggctttttca caacggatgc cgccggtgca
     2761 gtagatcagg attttttcgt ccttcttgtc cttcagcata tcggcaacaa tcggtaattc
     2821 ttcgcggaac gtgggcacat cggggcagat ggcaccttca aaatgcccga cctctgattc
     2881 atactggttg cgcatatcga cgacaacagt acccggctct tccagtgccc ggttgaattc
     2941 gagcggcttg aggtgtggcg cggcaacact cgcgtcgagc tcgaaatccc ccaggccgtc
     3001 gttgacaatg tagcgcttca ccttgatggt cagctttaga aacgaattac cgtcatcctc
     3061 gacgccggga ttaaagcgca tgctaccgaa tccgggccgg ccttcaaggg ctgcgcgaaa
     3121 tccgtcgagg tttgccaacg gcaccgaaag ctgcgcattg ataccttcac gcgccaggta
     3181 gatgcggcca cgcacgccca gctcagacca ctcgcggtag agctcgtccc tgagatcacg
     3241 gggattttca atcttcaggt aacggtagaa cgacagggtt cgccgcggca cgggcggttc
     3301 ggcagctatc tgcttcaaga gctgttcacg gctcagccgg ttgtgcagca tctcttttct
     3361 gaccgggtta cggtgcttag ccatacgctt cccggtaggc atcgccggcg ggcgagacac
     3421 gccgtcacgc gttgtcaaat ctgccctgct gaaggccagc cggccagcgg ttcaaggttc
     3481 acgggctgcg gcgggacgtc ggctgaaaag cccggcgagc aggctgccga tcaggctgtg
     3541 catgagcgat gatacggcgc atggcactgc cgccagcggg gtcaaatgtt tcatcgccag
     3601 cacaacaccc aggccagaat tctgcatgcc tacttcaatc gatatagtcc gcgcctgtgt
     3661 ttctggaatc cgcagcaacc gggaaaagac gtagccgaac agaaagccgc ccgcgtgcag
     3721 ggtgaagacc gcggcaatga tgctgaatcc gccggatatt atctgcgccc gcgaactgcc
     3781 gatgatcgag gcaacgatca gggtgatggc cagtactgcc aggaaatttg ccgagtgttc
     3841 aagttttttt gctgcatggg gtaacacccg ctttgccacg agtgcgagcg caatcggcag
     3901 taaaatcacc atcgccgtat cacgcgtcat cgcgagtgca tcaaccggga tacgcgcgcc
     3961 aatcagccag gctgagagcg cgggagtgaa catcgcagcc gcgatggtcg aaaccgcggt
     4021 catggttacc gacaggggaa catccgcctt cgccagaaaa gttatcacat ttgacgctgt
     4081 gccgcccggg caacaggcga caagaatcag acccgcagca atctctgctg gcaaagcaaa
     4141 ggccagcgcg gtaaaataac ccatcgccgg cataacggtg tactgtagca caaaacccgc
     4201 agccacctgc cccggtttgc gcgccacacg cttgaaatca gcagtggtga ggcttagccc
     4261 ggtgaagaac atgatcagcg ccagcgcgac cttgatatag agcgggccaa accaggtgaa
     4321 gatgggggga taaaaaaggg ctaacgcact cgctgccgcc gcgatgagcg ggaaaagctg
     4381 ggttagccgg ttcatggaga aggaaaatgc atcgtgcgcg tagcgcacaa tgcattttca
     4441 gatcttattg tggtgggtgc aggtcgcgtg ccccttcgta gatcacacgt ggacggaaca
     4501 ggcggttgtt ttcgtgctgt tcaatgtggt gtgaaacgag gcccgcgagg cgtgagcaga
     4561 ggaaaatcgg tgtgtagaga tcgatcggta cgttgatcag gtagtacagc agcgcgatcg
     4621 ggtagtcggc gttggggtac agaccctttt cgcgtgccat cgtcttctcg attgtctggt
     4681 agatcgagta gaggtctttg ccgtccggac gctggtcaac cagtttgggg atatagtctt
     4741 tcatgaggaa ggcgcgcggg tcgtacttct tcatgtaaac acggtggccg aagcccatga
     4801 tcttgtcttt acgctgcagt ttgcccatga tgtaactttc tgccgtagac ggaccgccgg
     4861 tcttctgcac ttcgaggaac atctgcatag ccgcttcgtt ggcgccaccg tggagtgggc
     4921 ctttgagcga agcaaccgcg ctcgcgcatg aaccgtaaat atcagagagt gttgagctca
     4981 ccacgcgtac cgcgaaggtt gagtttggca tttcgtgctc gatgtaggtt gtcagtatct
     5041 tgtcgagtgt atcggtttcg acagctgtcg gctttgaacc cttgatcatg tagacaaagt
     5101 tctctgagta gccgagcgac ttgtctggtt tcacgaaagg cagacccatg atagcgcggt
     5161 agctgttcac ggcaattgcg ggagctttgg ccagaatctt cagggccttc tcttcgttcg
     5221 attttgtcga agtatccatc agcgcagcgt ggtcttcata gccgccgagg atcgagatac
     5281 ccgtgcgcag tgcatccatc gccatggtct ttttcggcag cagcttgaag atttcgtaca
     5341 tctcttcagg aatatcagcc tgtgccatca gcttctgctc gaaagctttc gcttctgcat
     5401 ctgtaggaaa cttgttgtag atcagcaaat aggcaacttt gatgtaagat gtcttttgga
     5461 tcagctcgat gaggtcatac ccccgaacga taatctgctc tttttcttcg tcgagcagcg
     5521 aaagtttggt gatacaggcg ataacgccgt ctaaaccagg tttatagtct gcatgttcat
     5581 ttgccatacg gtttctcctc gtaatgttcc gaagctgacc gccccggggt tcttacgtgc
     5641 aaaagccaaa acagactcat agcgaccact tttttggagc ctaaaccttc attaagtctt
     5701 tacggcaggc cctgcatcgt gggcaggtta gagagtggcc gccgaacgcg tgcttttact
     5761 gcaggactgg tacgacgaaa acgacctgct gaatccttca tttttcgcac tgctcagcca
     5821 gtttcccgac gcacatgtcg gcttcctgaa aaatttcagc acaaccctac cagaggcgct
     5881 ggcgcagcgg cgtaccgaat tctatgccgc acggctgccc cggaagggtt tcgacaacct
     5941 cgaggcattg ataaaaatcc agtctgaaat ccccattgct gactacgacc tcgtcatctg
     6001 caatacccgc ggttaccttc gccacctgcg ccgcgaaaag gacgcccaca cacgcatcat
     6061 tgtctaccaa cacgacctgc tgcccttcct gtggcgggcc gacaccgaga ggctcaacac
     6121 ccaaaaggcg gcggagcttc tgcgactgca ggaaagtgac ctcgagtttg cggccggcat
     6181 cgatgtgatc gttgcggcca acttcgccct caaaacaacc ctggccgcca tgcaccggcg
     6241 cacggtaccg ctcgcctacc ccttggtcga cagcgacctg tttttccccg actctgaagc
     6301 agaggctgaa tattttgtcg caaccgacag tgcagacctt gaagcgctgc tgcacctgtt
     6361 cgcctgcgtc aatgacaaac tcgtagtcct gggcgactac cggcagaaca agcttttccg
     6421 cgagctgaag cccgacaaca ttttctatgc gggcaatctg ccagccactg aaaaggccta
     6481 ctacctggcg ggagccaaag ctgttttctg cggtgagacg cgcacactgg accatttgcc
     6541 gcttgctgcg ctcaagagcg gcattcctgt catcgcacac ccgacacagg gcatgcacga
     6601 attcctggtc gatggagatc tcggcgccga gctggaaacg ggatcagccg acgaactgat
     6661 tcgttacatt cgcttctacc gcgagcgcat gactgacaga gcaaaaatcg ccggcgaggt
     6721 cgattggctg aaccgcgagt actttctgcg ccgctggcgc aaggtactcg aacacggcca
     6781 gtgaaagcgc gtagattcat tatcttcagc ctggcagcaa ccgcgctcag tggcgcgcca
     6841 gaagaaaaaa aactccaccg tattctgttt agtggcctga cgcgcacgca cgagaatgtc
     6901 gtcaggcaat tcatcgcggt gaaaccgggt gaaccgatca ctgccgcaga cgcagaagag
     6961 ttgcgcacac agattctgcg cctgcgcatt ttttctgagg cacgcgttga actgcttgaa
     7021 cgcgacagcg ctgaaccgtg ccgcgcgctc tgcgacctgc atattaccgt gcgcgaaaaa
     7081 tggtcgctct acccggtacc agtgtacgtg cgctaccgcg ataccgaaat cgggggcgcg
     7141 tttgctgtcg aatcgaattt cctcgggcaa aacaagggcc ttgcggtggg agctct
//
LOCUS       DAOQJF010000158         7106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5852_length_7106_cov_5.499362, whole genome shotgun sequence.
ACCESSION   DAOQJF010000158 DAOQJF010000000
VERSION     DAOQJF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_7106_cov_5.499362"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..772)
                     /locus_tag="PLF85_09420"
     CDS             complement(41..772)
                     /locus_tag="PLF85_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19927.1"
                     /translation="MDGETLLVASRRFTMLEAERAEYLSAVNCLKAAAGLPVAPGRPI
                     RDLLREGSFEADLACALGSRILEAWPLAEQLGFLESLEPMAEAQKRRSGCISMRRSTN
                     CADGVWGLLIAELKGPVDVAAIEAWELSLRGALDQLPDNTALKILVDLVGLAPVDVET
                     HKRYRSIVPLALAKHGWRIGYLDMFEEANHLPLTTERGVHCVKAAHAHQDAYKIEEYD
                     RRFGKASERFFTDSAAAFQWLRARP"
     gene            1070..4243
                     /locus_tag="PLF85_09425"
     CDS             1070..4243
                     /locus_tag="PLF85_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19928.1"
                     /translation="MLEYALPVAASLFFTLFLRRLDKSNVNLKKIKAIAERAERELAE
                     LTLKKKEELKDATVRFDSLLINADKYLGMLHEKLEQAKTDLVSVESTRQNLRVMDSEL
                     QSLDNTTRSVKDQLKFINDSLDKIDQQQKKIKKLQEHVKQVDDEAANMIDAFQAALKD
                     KSGEILVVLEKKFEEITNETLRMQGELRDDLMARHIDLKGEVKHSYEQLEIDLKKSAQ
                     DLTLNIESRIQAQIQAVTDLQERIFSAEKNLEVTIPDMIRDIKNDLLRDMHEQAQKLE
                     TLKLTLGGTEDTMRKKLQAFREELEQQRSLLFHEIAAEAERIRDQISNLDLDALAKKD
                     EIIKSARSEAQKIQKNIEEFNDQYSRARETLFQEASRRENELTRRLDEIEDLSKGISA
                     QLSQNRSRGLEEMQNELQKALSEIKAASRELFSDIEEEILRESGMLREKLTSVQAEVT
                     QIVQQTEERCENLETKVADISQSTEKEFANYLKELKKAAEKGLNDYREELSERERQFM
                     QSLTSLDKSLRDKIADADRHFESAQARLEEIVKDARNALHKNNLEIEKNRAELMRDMD
                     SELEDRLSHARHFIEEAQEKSANAFADAFSKFRTRIEEKENEFSSKIELNIARFFDMK
                     KSLEASQENLIENLQSEKRRIEAEMRQANDAQLKLFDQELNERETRHRQQLISMGSES
                     VSDFRSKVSELMDRLVEMKSDTTSTFREFQENQQEVYAAIVEETRATGEEIRDLKEAL
                     RELKAEGMSIEKSKQSAETMRALIRELAQKIEQAESKNQVLGEVYQRVEELKEMRTKL
                     ETELLMLKEKRDKVDRLEESINFVLSMQNEIEAKSIQLKKLQTNLDEFSAHYARLDEE
                     RRKADVLMQNLIDENRLIQKTIDKIEAQDKHIDSLNEGIAQLNGFFQRVEQRSHLLKD
                     QLDTITEQMMSLHKNEQELQSVQSRFMEIEDLMADIEKKKTQIIAFNKQFEELRKQMS
                     TSVQQIERIEQNAESKVRQLSEFMKALDDETEKKVTLTKTLSPAGVGDKKDMVRKLSN
                     FGWTAEEIAARVNLDINTIETILSMPG"
     gene            4337..6397
                     /gene="recG"
                     /locus_tag="PLF85_09430"
     CDS             4337..6397
                     /gene="recG"
                     /locus_tag="PLF85_09430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNE19929.1"
                     /translation="MISLSETGLFSAEQLKVLSRLKVKDVRDLFYYLPRKYIDRSQQL
                     DLRNSRAGDLVTVVGKIMHAEMKYAGRRRFTIKVEANGFIIYATFFNAGAYLQKVLAV
                     GKEIACWGKLEVFHGRLSFMHPEWEVLTEEGTKHMGRIVPIYRITEAMRTAFLTVKTL
                     REKIEACLERYEKRITDYIPPNNRKAWQLLGVAEALHKIHFPETLEETEEARRRMAFD
                     ELVLFSVLNEEKKAAVRRIEKDFRLVLTPAISREVDELIKGLPFELTADQQMAVETLF
                     KNSTATYPVNHLLMGDVGSGKTLVALLLALFYMRQGLQVAYLAPTEILARQHYQTFLN
                     YLPKGSFEGVEIIIGKDAAKDRRAKLDRLARGESLIAIGTHALIQDDVTFKNLALIII
                     DEQHRFGVEQREKLRAKGKSPDLLSMTATPIPRTLTLAYYGDLEPIYLKQKPKNRLKI
                     DTRIFNEIELERIYKAVRKYVEQGRQVYIIYPVIDESENSDMASLLSDFATLEKETLP
                     DLRLGLLHGRLSGDEKDRAMQKFKEGLIQVLVATSVVEVGIDVPNANVIVIRHPDRFG
                     LSQLHQLRGRVGRGEHQSFCILVAPEKITEEGRRRLDAMVRTDDGFELAQIDFELRGA
                     GELTGLRQSGASEFHIADLRVHGALLERAGQLLQEHPEIQAQFGAMKNIRQTLEKGLV
                     LFGN"
     gene            complement(6394..>7106)
                     /locus_tag="PLF85_09435"
     CDS             complement(6394..>7106)
                     /locus_tag="PLF85_09435"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNE19930.1"
                     /translation="KIPDDAKIGPYVVIERGAVIGKGCVLQAHVVIEFEAVIGDGTVI
                     QPGSVIGFRCKIGKNCLILPNSVIGSEGFGYAQDEEFNHYRIPQTGDVVIGDRVTIGA
                     LNAIDRATFGSTVFHENVICDNICHTAHNCEIGAHSILLTGWKMAGSSKTGKRVIASG
                     DAMVKDHVSICDDVVLVHRAGVIADITEKGMYAQSPTQPMREYVKNWGAYQKLGDMAK
                     EMKELKKQVAELLVAKGA"
BASE COUNT         1877 a         1997 c         1911 g         1321 t
ORIGIN      
        1 aaagcctctg cagcccgccg gacggctctt ccgtagtctg ctagggcctg gcgcgcagcc
       61 actgaaaggc ggccgccgaa tctgtgaaaa acctttcgga ggcttttcca aaccggcgat
      121 cgtactcttc aatcttgtaa gcatcctggt gcgcgtgggc ggccttgacg cagtgtacac
      181 cgcgctctgt ggttaagggc aaatggtttg cctcttcgaa catgtcgaga taaccaatgc
      241 gccaaccatg ttttgccagg gcgagcggaa caatgctacg gtaacgttta tgcgtttcga
      301 cgtcgacagg ggccaagcct acgagatcga caagaatttt gagagcggta ttgtcaggca
      361 actggtcgag ggcgccgcgc aacgaaagct cccacgcttc gatcgctgca acatcgaccg
      421 gtcccttgag ctcagcgatc agcagacccc aaaccccgtc ggcacagttc gtagacctgc
      481 gcatagatat gcacccgctt ctgcgcttct gcgcttctgc catcggctcg agggactcga
      541 gaaacccgag ctgctcggca agcggccagg cttcaagtat gcgcgaaccc agcgcgcacg
      601 ccaggtcagc ttcaaacgag ccctcacgca agaggtcgcg tattggcctg ccgggagcta
      661 ccggcaatcc ggccgctgcc ttgagacagt tgactgctga aaggtactcg gcacgctcgg
      721 cttcaagcat ggtgaaacgc cggctcgcga ccaataaggt ttctccatcc atagctccca
      781 ctcggcaggc gaaacgaagc gtcaagctat catttcgttg attgactaaa tataaattaa
      841 aacgaaagcc cagccatcct gccgacgacc caagtcgaga accgggcgca ctctagcgga
      901 agagaagttg cggccgcagc aggagctgtg gcatgtgaaa ttcccgggca gtggcgttac
      961 acgccgttcg tgtaacgtct gcttttatgc cttttgcggc ccattttacc tcgtccctct
     1021 ttagaaaaag gcttgtaaac gcaggtgcgg cgcaataatg tgacataata tgctcgagta
     1081 cgccctgccg gtggcggcat ctcttttctt cacacttttc ctgcgccgcc tcgacaaatc
     1141 aaacgtcaac ctgaagaaaa ttaaggccat tgctgaacgg gcagagcgcg aactggcgga
     1201 gcttaccctc aagaagaaag aagaacttaa agacgcaacg gtccgcttcg attcactact
     1261 gattaacgcg gacaaatacc tcggcatgct gcacgagaaa ctcgagcagg cgaagactga
     1321 cctggtatct gtagaaagca cgcgccagaa cctgcgcgtg atggacagcg aactgcagtc
     1381 gctcgacaat actacccgct cggtcaaaga ccagctgaaa tttatcaacg actctctcga
     1441 caagatcgac cagcagcaga aaaaaatcaa gaaactgcag gaacacgtga agcaggtaga
     1501 cgacgaagca gccaacatga tcgatgcgtt ccaggcagcg ctaaaagaca agtcgggcga
     1561 aattcttgtg gtgctcgaga agaaattcga agagatcacc aacgaaacac tgcgcatgca
     1621 gggtgagctg cgcgacgacc tgatggcccg ccacatcgac ctcaagggtg aagttaaaca
     1681 cagctacgaa cagctcgaaa tcgacctgaa gaaaagtgca caggacctca cgctcaatat
     1741 cgagagccgc atacaggcgc agatacaggc cgttaccgac ctgcaggagc gcatcttctc
     1801 ggcggaaaaa aatctcgagg tcactattcc cgacatgatc agggatatca agaacgacct
     1861 gctgcgcgac atgcatgaac aggcacagaa gctcgagacg ctgaagctga cgcttggcgg
     1921 taccgaagac acgatgcgca agaagctgca ggcattccgc gaagagctgg agcagcaacg
     1981 ttcattgctg tttcatgaaa tagcagccga ggctgagcgc atacgtgacc agatttctaa
     2041 cctcgatctt gacgcactcg ccaagaaaga cgaaatcatc aaatcggcgc gcagcgaagc
     2101 gcagaagata cagaagaaca tcgaagagtt caacgaccag tacagccgcg cgcgcgaaac
     2161 actttttcag gaagcttcgc gccgcgaaaa cgagctgacg cgtcgcctcg acgagatcga
     2221 agacctcagc aagggcatca gcgcgcaact ttctcaaaac cgttcccgcg gccttgaaga
     2281 gatgcagaac gaactgcaga aagcactcag cgaaattaag gcagcatcgc gcgaactttt
     2341 cagcgacatc gaagaagaga tcctgcgcga gtcgggaatg ctgcgcgaga agctgacctc
     2401 agtacaggcc gaagtaacgc agattgtcca gcaaaccgaa gaacgctgcg aaaacctcga
     2461 gaccaaagtc gccgatattt cacagagtac cgagaaagaa tttgccaact acctgaaaga
     2521 gctcaagaaa gccgcggaaa aaggactcaa cgactaccgc gaagagctca gcgagcgcga
     2581 acgccagttc atgcagagcc tcaccagcct cgacaagagc ctgcgcgaca agatcgccga
     2641 cgccgaccgc cactttgaaa gtgcacaggc gcgcctcgaa gaaatcgtca aagatgcccg
     2701 caacgcgctg cataagaaca acctcgaaat cgaaaaaaac cgcgccgagc tgatgcgcga
     2761 catggactct gaactcgaag atcgcctgag ccatgcgcgg cactttatcg aagaagcgca
     2821 ggaaaaatca gccaatgcct tcgccgatgc attctcgaaa ttccgtaccc gtattgaaga
     2881 gaaagagaac gagttcagct cgaaaattga actgaacatc gcccgcttct tcgatatgaa
     2941 gaagagcctc gaagcatcgc aggaaaacct gatcgagaac ctgcagtcag aaaagcgccg
     3001 cattgaggcc gaaatgcgcc aagcgaacga cgcgcagctg aagctcttcg accaggagct
     3061 caacgagcgc gaaacccgcc accgccagca gctgatctcg atgggctctg aatcggtcag
     3121 cgatttccgc agcaaggtca gcgaactgat ggatcgcctg gtcgagatga agtccgacac
     3181 cacttctacc ttccgcgaat tccaggaaaa ccagcaggaa gtgtacgcgg caatcgtcga
     3241 agaaacccgc gccacaggcg aagagattcg cgacctgaaa gaagcactgc gggagctcaa
     3301 ggccgagggc atgagcatcg agaagagcaa acaatccgcc gaaaccatgc gcgcactgat
     3361 tcgcgagctg gcacagaaaa tcgagcaggc agaaagcaag aaccaggtgc tcggtgaagt
     3421 ctaccagcgc gtcgaagaac tgaaagagat gcgcacgaaa ctcgagaccg aactgctcat
     3481 gctcaaagag aagcgcgaca aggtcgatcg cctcgaagaa agcatcaact tcgtcctctc
     3541 gatgcagaac gagatcgagg cgaaatccat ccaactcaag aaactgcaga cgaacctcga
     3601 tgaattctct gcgcactacg cgcggctcga tgaagagcgc cgtaaggctg atgtgctgat
     3661 gcagaaccta atcgacgaaa accgcctgat acagaagacc atcgacaaga tcgaagccca
     3721 ggacaagcac atcgattcgc tcaacgaagg tattgcacag ctcaacggct tcttccagcg
     3781 tgtcgagcag cgctcgcacc tgctgaagga ccagctcgat accatcaccg agcagatgat
     3841 gagcctgcac aagaacgagc aggagctgca atcggtacag agccgtttca tggagatcga
     3901 agatctcatg gcggatatcg agaagaagaa gacacagatc attgcattca acaagcaatt
     3961 cgaagagctg cgtaagcaga tgtcaacgag cgtacagcag atcgagcgta tcgagcagaa
     4021 cgccgaaagc aaggtgcgcc agctttctga gttcatgaag gcgctcgacg acgagaccga
     4081 gaagaaagta actctgacga agacgctctc gcccgcgggc gtcggcgaca agaaagacat
     4141 ggtccgcaag ttgtcgaact tcggctggac ggcagaagaa attgctgcac gtgtcaatct
     4201 cgacatcaac acgatagaga ctattctctc gatgccgggg taatcccagc tgaaactccc
     4261 tgcactgaaa cgagcgcagg gcacgtggag caacagcagc cgcgatcact gcgctagacc
     4321 gctggcataa gtaaaaatga tatcactctc tgagacaggc ctgttttctg cagaacagct
     4381 caaggtgctt tcgcgcctca aggttaaaga cgtccgggat cttttctact acctgccgcg
     4441 caagtatatc gaccgttcac agcagcttga cctgcgcaat tctcgggccg gtgacctggt
     4501 gactgtcgtt gggaaaatca tgcacgcgga aatgaagtac gcaggccgac ggcgctttac
     4561 aatcaaagta gaagccaacg gttttatcat ctatgccacc ttctttaacg ccggcgccta
     4621 cctgcagaaa gtcctggccg tcggcaaaga gatcgcctgc tggggcaagc tcgaggtatt
     4681 tcacgggcgg ctgagtttca tgcaccccga atgggaggtt ctgactgaag aaggcacgaa
     4741 gcacatgggt cgcattgttc ctatctaccg catcaccgaa gcgatgcgta ccgcttttct
     4801 tacggtcaaa acgctgcgcg aaaagattga agcctgcctc gagcgctacg aaaaacgtat
     4861 caccgattac attcctccaa ataaccggaa ggcctggcaa ctgctgggcg ttgctgaggc
     4921 tctgcacaag attcattttc ccgaaaccct ggaagagact gaagaggcac gccgccgcat
     4981 ggcgttcgat gagcttgtac tcttctccgt actgaacgaa gagaaaaaag ccgcggtgcg
     5041 ccgcatcgag aaggactttc gccttgtcct gacgccggct atcagccggg aagtcgatga
     5101 actgatcaag ggattaccct ttgaactgac ggctgaccaa caaatggcgg tcgaaactct
     5161 cttcaaaaat agtacagcaa cctacccggt gaatcacctg ctgatgggtg atgtcggcag
     5221 cggcaagacg ttggtggcgc tgctgcttgc gctgttctac atgaggcagg gcctgcaggt
     5281 cgcttacctc gcaccgaccg agattctcgc gcggcagcat taccagacgt ttctcaacta
     5341 cctgcccaaa ggttcgttcg agggagttga aatcattatc ggcaaagacg cagcaaaaga
     5401 ccgccgggca aaactcgacc gcctggcgcg cggcgaatcg ctgattgcga ttggtacgca
     5461 tgcattgatc caggatgatg taaccttcaa aaacctcgcg ctgatcatca tcgatgagca
     5521 acaccgcttt ggtgtagagc agcgcgagaa gctgcgtgcg aaaggtaaat cacccgacct
     5581 cttgtcgatg acagccacgc cgataccccg cacgctcaca ctcgcctatt acggtgacct
     5641 cgaacccatc tacctgaagc aaaagcctaa gaaccgcctg aagatcgaca cgcgcatctt
     5701 taacgagatt gaactcgagc gtatctacaa ggcagtgcgc aagtatgttg aacagggcag
     5761 acaggtctac atcatctacc cggtgattga tgagagtgaa aactccgaca tggcatcgct
     5821 gctgtctgat tttgcgacgc tcgagaaaga aacattgcct gatctacgcc ttggactttt
     5881 acacggccgc ctcagtggcg atgaaaaaga ccgcgcgatg cagaagttca aagagggatt
     5941 gatccaggta ctggttgcaa cgtccgttgt ggaagtcggc atcgacgtgc ccaacgccaa
     6001 cgtgatcgtg attcggcatc ctgaccggtt cgggctttcg cagctgcacc agctgcgcgg
     6061 gcgcgtcggc cgcggcgagc accagagttt ctgcatactc gttgccccgg aaaagattac
     6121 cgaagaaggc aggcgcaggc tcgacgcgat ggtgcgcacc gatgacggct ttgagctcgc
     6181 gcagattgac tttgaactgc gcggtgcggg tgagctcacc ggcctgcggc agtcaggcgc
     6241 gtcggagttt catatagccg acctgcgcgt acacggcgca ctgctcgaaa gagcaggcca
     6301 gctgctgcaa gagcacccgg aaattcaggc gcagttcggc gccatgaaga atattcgcca
     6361 gaccctcgag aagggcctgg tgttgtttgg taattaagcg cccttcgcta ccagcagctc
     6421 cgctacctgc ttcttgagct ctttcatttc tttcgccatg tcaccgagct tttggtaagc
     6481 accccagttc ttcacgtact cgcgcatcgg ctgcgtgggt gactgcgcat acataccctt
     6541 ttcggtgatg tcggcgatga caccggcacg gtgtaccagc accacgtcgt cgcagatgct
     6601 gacgtggtct ttgaccatcg catcgccgct tgcaatgacg cgtttaccgg ttttcgagga
     6661 gccagccatc ttccagccgg tgagtagaat ggaatgcgcg ccaatttcgc agttgtgcgc
     6721 cgtgtggcaa atgttgtcgc agatcacgtt ttcatggaaa accgtgctgc cgaacgtagc
     6781 acggtcaatg gcgttcagcg cgccgatggt cacacggtca ccgataacga catcaccggt
     6841 ctgcggtatg cggtagtggt tgaattcttc gtcctgcgca taaccgaaac cttctgaacc
     6901 aatcacgctg ttaggcagta tgaggcagtt cttgccgatc ttgcagcgaa aaccaatcac
     6961 gctgccgggc tggatcacgg taccgtcgcc gattaccgcc tcgaactcaa tcacgacatg
     7021 tgcctgcagc acgcagcctt tgccgatcac cgcaccacgc tcaatgacga cgtacggtcc
     7081 aatctttgca tcgtcgggga tcttca
//
LOCUS       DAOQJF010000159         7041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5929_length_7041_cov_5.303750, whole genome shotgun sequence.
ACCESSION   DAOQJF010000159 DAOQJF010000000
VERSION     DAOQJF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_7041_cov_5.303750"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLF85_09440"
     CDS             <1..800
                     /locus_tag="PLF85_09440"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE19931.1"
                     /translation="HGTNIKDFAKTRYEHWLTMVRETYLRERPRFRHFFLIGFSMGGN
                     LCLNLATELAGRTPIQRGSNASGFADSRNGSLKPTGIVTIASPVVLNGFLNGTLIIKD
                     PRLFLSGIMKNFFRHIPKAKSVVAEHVMIPWVGYGEAYTLAPLHSFKLGVASVRTRLH
                     RIRQPLCMIHATGDKTVHIENMHYIFRKVASTEKRAYVFSLNEELSTHHVLVTHQLAR
                     DKVFHYIMKFVHDTLRGFDLKPAIFNPGGDKSRWVDKLRRFRGFLSG"
     gene            810..1754
                     /locus_tag="PLF85_09445"
     CDS             810..1754
                     /locus_tag="PLF85_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE19932.1"
                     /translation="MGYDGSLYFLRDELLPEFRDELRQKSLKAEEIAPGVYFAPGVQR
                     LYWVLDYWPVRRLKFVSVSDGAKQLRRLGKRWVYVGAQAFRRGQLVADELRVKPLKQL
                     AFPVEADDRACGAFTLASADEIFWTDAPLKARFAGGKIRFNEDRTGPPSRAYLKLQEA
                     LTLAGINLSATDTVLDLGATPGGWSYVAAQAGAQVEMIDRSPPDERLFKRFARLSFRR
                     GDGLNPSHELLASATVLLSDMACEPAKLLDAVQAWLALPNVRAMVCTLKFHGLSDKKL
                     LERFAAVTGAEIYHLWYNGHEVTWVWKREVIPASSGIG"
     gene            complement(1726..2121)
                     /locus_tag="PLF85_09450"
     CDS             complement(1726..2121)
                     /locus_tag="PLF85_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19933.1"
                     /translation="MTKKSPKKTLSKKSPKAEPLFAPKCKNCSGFEEKLSVVEHNEVK
                     DEVSLMHKHEIIRQMSAFIGDNAQSLMQAEEIFDRFRYMMAEYVLKHEFNCESKLSVQ
                     MVLWLEQKCEPTAAAPLHNIISQYLTKRV"
     gene            2349..3812
                     /locus_tag="PLF85_09455"
     CDS             2349..3812
                     /locus_tag="PLF85_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19934.1"
                     /translation="MRFTSFTLPLLLVLPLSVLSAESSKDVAKDKTEKASDLARVAVM
                     TFINEGNNVDYEWVEKSLPDAINDSMKARFEFARQDESKVTAIAAKYKHAGGEYQIAD
                     ADKIAKESQTDILIYGNFRTNDKKDQLLFKAIIYNAQGKKVIGVVEDSSPLDAKIFKA
                     IDRMAAGIVEKIYGFALAAEREKGKQNELKILVLVPSYTTAEEEKQAIRELEVLKSEL
                     SETYIGRYLTIYEFYKEYKVNREEQEKSLGYAKKRERDKIVNWLEGYGVRNAFIVLVA
                     DKKVAITPVAQGKPAPPVTYAVNAKPEEKRASIAQASKEVTPKNEKVELKKDTFTNTS
                     VTLLHVGLLGGKGILDAGNNLGPMTGLSAQFGMRIWKPWFQPQVKFEGYYVLQKEPVN
                     YLAGGSLAGGLGYTWLLGSRMALTPYVMGGVFAGVIKNTATNVSFNLPLVAAGLTYTY
                     FFSARWGISLNSHTQYVIDSAAPGLFFTGALSWVYRF"
     gene            3896..4345
                     /locus_tag="PLF85_09460"
     CDS             3896..4345
                     /locus_tag="PLF85_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19935.1"
                     /translation="MLNVKSLIVLVASAVLALNCGTSAKSQQLKDSNGKVIGRYDVLS
                     DSEAKANFDVNQNGVNERVASYKDQKLVAVDYFDDANGTKTKAVQFKDGKPESVKVFD
                     KNGKEVRGDVAYDTAKNSAKEVTLPAKSKKVIFNGDGTVSVTNIEKK"
     gene            complement(4396..5115)
                     /locus_tag="PLF85_09465"
     CDS             complement(4396..5115)
                     /locus_tag="PLF85_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNE19936.1"
                     /translation="MKFYRVLSLAAFVAAASALHATEPDYSAWNSLLRKYVHAGTKAG
                     IKANLVRYAALSKDPLWPAAQAALTGFDPEKLSGKKERLAFWINAYNIAAVRKVLEIY
                     PTTSITARGDGVWKAPAIRIAGKDYSLDEIEHKQLRPLGDARIHFAIVCASLSCPDLR
                     TEAYTAAQLEKQLGAQTRAFVANKTKGVRIEADAVCVSAIFNWFAQDFGDVAKFIARF
                     GAKLPGGLARKEISYNWNLNE"
     gene            complement(5112..6431)
                     /locus_tag="PLF85_09470"
     CDS             complement(5112..6431)
                     /locus_tag="PLF85_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE19937.1"
                     /translation="MAVRRVLAQAAGGQLSVKVVIVGNGIAGTTAALRLRQLAPEAEI
                     TLISEETPLFYSRTALMYIYMRQLQFADTVVHPADFYRKQRINLQQDLVTSLDLQSRR
                     LTGESATYSYDYLLLAPGSRPRQPGLAGENLPGVQGLYSKADLDRLEEQTRTPVASAV
                     IAGGGLIGVELAEMLGSRRIPVTMLVREPLYFSHILPREEAEMVTNEVGKHVDLQLGT
                     TLSEIRGDNTVRSVVTSRGEEISCTLLGLTIGVEPRTELARAAGLAVKRGIITDEYLR
                     TADERVYAAGDAAELPLPGGDSKVQQIWYTGRLQGEVAGANLAAAIKGKTPTAYDSGI
                     YFNSARFFSLDYQTYGAVPVAADKSSSLLWQNVAEKKLLRISYDATAPGRPVTGFNAL
                     AVRLLHPVCEAWIRDRTPLETVVQKFDRAVFDPEFSKRYTKELRSLL"
     gene            complement(6379..>7041)
                     /gene="arsS"
                     /locus_tag="PLF85_09475"
     CDS             complement(6379..>7041)
                     /gene="arsS"
                     /locus_tag="PLF85_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777029.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNE19938.1"
                     /translation="GKHLIDRCNLTILSEPGHEDLADFLAANEVEIVASLPHFAEQRT
                     DSQRGAGVFQKSIAGLKKLNALGYGAQLKLNLVYNPSGVFLSSNQLQLEREFREKLMA
                     DHGITFNSLYCINNMPINRFLFALERAGKTATYLTTLVNAFNPQTIPGLMCRTQISVG
                     YDGQVYDCDFNQMLDMPAEAVAHIDDFTPERFSSRSIRTANHCYGCTAGSGSSCGGAV
                     VS"
BASE COUNT         1572 a         1902 c         1990 g         1577 t
ORIGIN      
        1 gccatggtac caatattaaa gatttcgcca aaacgcgcta cgaacactgg ctgaccatgg
       61 tgcgcgagac ttacctgcgc gagcggccgc gcttcaggca tttttttctc atcggctttt
      121 ccatgggagg taacctgtgc ctcaacctcg cgaccgaact ggcggggcgc acccccatac
      181 aacgcgggag caatgcatcg ggttttgccg acagccgtaa cggctctctg aagccaacag
      241 gtatcgtgac gattgcttcg ccggttgtgc taaatggttt cctgaacggt acgctgatca
      301 tcaaagatcc gcggcttttt ctctcgggta tcatgaagaa tttcttccgc catataccga
      361 aggcgaagtc ggtggtggcg gagcatgtaa tgattccgtg ggtcggctac ggtgaggcgt
      421 acacgctggc gccgctgcat tcgttcaagt tgggtgtggc gagcgtccgt acaaggttac
      481 accggatcag gcagccgctc tgcatgattc atgcgacggg tgacaagacc gtgcacatcg
      541 aaaacatgca ctatatcttt cgcaaggtgg cttcgactga aaagcgcgct tatgtcttta
      601 gcctcaacga agagctgagc acacaccacg tgttagtgac gcaccagctt gcccgcgaca
      661 aggtattcca ctacatcatg aaattcgtgc acgacacgct ccgcggtttt gatctgaagc
      721 cggcaatttt taatcccggt ggcgacaaga gccgctgggt tgacaagctg cggcgcttcc
      781 gcggctttct atcgggttga gctccggttg tgggttacga cggttcactc tatttcctgc
      841 gcgacgaact tctgccagag ttcagggatg agctccgcca aaaatcgctc aaagcggaag
      901 agatcgcccc gggtgtatac tttgcgccgg gcgtgcagcg tctttactgg gtgctcgact
      961 actggcccgt caggcgtttg aagtttgttt cggtgtctga tggggcaaag cagctgcggc
     1021 ggctgggtaa acgctgggtg tatgtgggag cgcaggcgtt ccggcgcggc cagctggttg
     1081 ccgatgaact ccgcgtgaag ccactcaaac aactggcgtt tcccgtcgag gcagatgacc
     1141 gcgcctgcgg tgcctttaca cttgcatctg ccgatgaaat cttctggaca gatgcgccgc
     1201 tcaaggcacg tttcgccggc ggcaaaatac gcttcaacga agaccgtacc ggcccgccca
     1261 gccgtgccta cctgaaactg caggaggcac tgacgctcgc gggtattaat ctcagcgcga
     1321 ccgacacagt actcgacctg ggtgcgaccc ccggcgggtg gagctacgtt gcggcacagg
     1381 cgggcgcaca ggtcgagatg atcgaccgca gcccaccgga cgagcggctc ttcaagcgct
     1441 tcgcgcggct cagttttcgg cgaggtgacg gcctgaatcc atcgcacgag ttgctcgcat
     1501 cggctacggt gctcctcagc gacatggcct gtgaaccagc caagctgctc gatgcagtgc
     1561 aggcctggct ggctttaccc aatgtgcggg cgatggtctg cacgctcaaa ttccacgggc
     1621 tgtcagataa gaaactgctg gaacggtttg cggccgttac cggcgcagaa atctaccatc
     1681 tttggtacaa tggtcacgaa gtgacctggg tatggaagag ggaggttata cccgcttcgt
     1741 caggtattgg ctgatgatat tgtgcagcgg tgcagcagcc gtcggttcgc atttctgctc
     1801 aagccacagc accatttgca cggaaagttt gctctcgcag ttgaactcgt gcttcagcac
     1861 gtactctgcc atcatgtaac ggaaacggtc aaaaatttct tctgcctgca tcagcgattg
     1921 ggcattgtcg ccaatgaatg ctgacatctg ccggataatt tcgtgtttgt gcatcaacga
     1981 gacttcgtct tttacctcgt tgtgttctac cacagagagt ttctcttcaa agcctgagca
     2041 gttcttgcat ttgggggcaa ataatggctc ggcttttggc gattttttgc tcagggtctt
     2101 cttcggagat tttttagtca cgttagttcc ggcacgcttt tgatggggca aatatacgca
     2161 gtggtgggct cggtgtcgat tttttgtatt aaccgcttgg ttaggccacc ttgaattccc
     2221 tcttttgatt cccggctgcc gcccggcctg cagtctctga gggcatcgga tatttatcgg
     2281 gtaactggcg gcacagtctc gcccgaaaga agttgtaatt tcgggtcagc cgcagtttct
     2341 gcctgcctat gcgatttacg tcctttactc ttccgttact tctggttctc ccactctcgg
     2401 tcctgtctgc cgaatcgtca aaggatgtcg caaaagataa gacagaaaaa gcgtccgacc
     2461 ttgcgcgtgt tgccgtcatg accttcatca acgaaggcaa taacgtcgac tacgaatggg
     2521 tcgaaaaaag cctgcccgac gcgattaatg actcaatgaa agcccgtttt gaatttgccc
     2581 gccaggacga aagcaaggtt accgcaattg ccgcaaagta caagcatgcc ggtggcgaat
     2641 accagatagc tgacgccgat aagatcgcca aagaatcgca gactgatatc ctgatttatg
     2701 gaaatttccg cacgaacgat aagaaggacc agctgttgtt caaagcaatc atctataatg
     2761 cgcagggaaa aaaggtcatc ggggtcgtcg aagattcgag cccgctcgac gccaagatat
     2821 tcaaagccat cgaccggatg gccgcgggta tcgtggagaa gatctacggc tttgcgcttg
     2881 ctgccgaacg ggaaaagggc aaacagaatg agctcaagat actggtgctc gtgccttctt
     2941 acacgaccgc agaagaagag aaacaggcaa tccgcgagct cgaggttctt aagtcagagc
     3001 tcagtgagac ctatatcggg cgatacctga cgatctacga gttctataaa gaatacaagg
     3061 tgaatcgcga agaacaggaa aaatccctcg gctatgcaaa aaagcgggaa cgcgataaga
     3121 tagtcaactg gctcgagggt tacggcgtgc gcaacgcatt tatcgtactc gtggcagata
     3181 agaaggtggc catcactccg gtggcacagg gcaagcctgc cccgccggtg acatatgcag
     3241 tgaatgcgaa gcccgaagaa aagagggcca gcatcgcaca ggcttcaaaa gaagtcacac
     3301 caaagaacga gaaggtcgaa ctcaagaaag atacgttcac caatacttcg gtaaccctgc
     3361 tgcatgtcgg tctcctgggc ggcaaaggca ttctcgatgc cggcaataac ctgggtccga
     3421 tgactggttt gagcgcacag tttggtatgc gcatctggaa accctggttc caaccgcagg
     3481 tcaagtttga aggttattac gtcctgcaga aagaaccggt gaattacctc gcaggcggct
     3541 cgcttgccgg tggactaggc tacacgtggc ttttgggtag tcgcatggcc ctgacgccct
     3601 acgtgatggg gggcgttttt gcgggagtca tcaagaatac cgcgaccaac gtgagcttca
     3661 acctgccgct ggttgcagcc ggactcactt acacctattt cttcagcgcc cgctggggaa
     3721 tcagtctgaa ttcccatacg caatacgtca tcgacagcgc tgccccgggc ctcttcttca
     3781 ctggcgcgct gtcctgggtt taccgcttct gacaagcacc cctgggaaat agaacgggct
     3841 gacttccagt tagccattag acttgacgat acactatgga gaaaggagct agaccatgct
     3901 aaatgtaaaa tccctgatag ttcttgtggc gtcagccgta ctggcgctga attgcggtac
     3961 ctccgccaag agccaacagc tgaaagacag caacggaaaa gtgattggcc gctacgatgt
     4021 gctgagcgat agcgaagcca aggctaactt tgacgtcaac cagaacggcg taaacgagcg
     4081 cgtggcctcg tacaaagacc agaaactggt ggccgttgat tatttcgacg acgccaacgg
     4141 tacaaaaacc aaggctgtcc agttcaaaga cggcaagcca gagtcggtaa aggtttttga
     4201 caagaatggc aaagaagtcc gcggtgatgt cgcttacgac acagccaaga actcagctaa
     4261 agaagttacc ctgcctgcaa agagcaagaa agttatcttt aacggtgatg gtacagtttc
     4321 tgtaaccaac atcgaaaaga agtaagttac gcttccctta ccctgcgcgc tgcgcagggt
     4381 aacgcgtgtt ccctgctact cgttcagatt ccagttgtaa gaaatctctt tcctggcgag
     4441 cccgccaggt agtttcgcac cgaagcgtgc tataaacttc gccacgtcgc caaaatcctg
     4501 cgcaaaccag ttgaatatcg cagaaacgca taccgcgtct gcttcaatac gcactccctt
     4561 ggtcttgttg gccacgaatg cgcgcgtctg cgcccccagc tgtttctcca gctgagccgc
     4621 cgtgtacgcc tcggtgcgca ggtcgggaca gctgagtgaa gcgcagacaa tcgcgaagtg
     4681 gatgcgtgca tcacccagtg ggcgcaattg cttgtgctcg atttcgtcca gcgagtagtc
     4741 tttgccagcg atcctgatcg caggtgcctt ccagacgccg tcgccccgcg cggtgatgct
     4801 ggtggtaggg tagatctcga gcactttgcg gaccgcggca atattgtaag cattgatcca
     4861 gaaggccagg cgctccttct tgccggataa tttttcggga tcaaaacccg tcagcgcggc
     4921 ctgtgcagcg ggccacaaag ggtcttttga gagtgctgcg tagcgcacga ggttggcctt
     4981 aataccagcc ttcgtgcctg cgtggacgta cttcctgagc aggctgttcc atgcactata
     5041 atcgggttca gttgcatgca gcgcagaagc agctgcaacg aaggctgcca acgacagtac
     5101 tctgtaaaat ttcataacag actcctcaac tccttggtgt agcgtttgga aaattcggga
     5161 tcaaagaccg cgcggtcaaa tttttgcact accgtctcga gcggggtcct gtcgcgtatc
     5221 caggcttcgc agaccgggtg cagcaaacga acagccagcg cgttaaagcc ggtgaccggc
     5281 cgacccggcg cagtcgcgtc gtagctgatg cggagtagtt tcttttcagc gacattttgc
     5341 cagagcaggc tgctgctttt atctgcagct acgggtacgg caccgtaggt ctggtaatcc
     5401 agagaaaaga agcgggcaga attgaagtag atgcctgagt cataagcagt gggagttttc
     5461 cctttaatcg ccgcggcaag attcgcacct gcgacttcac cttgcaggcg gcccgtgtac
     5521 cagatttgct gcaccttgct gtcaccgccg ggcaggggca actcggcggc gtcaccggcg
     5581 gcatagactc gctcatctgc ggtccgcaaa tattcgtcag taatgatgcc gcgcttaacc
     5641 gcaaggcctg ccgcgcgtgc gagctcagtg cgtggttcaa caccgatggt gagcccgagc
     5701 agcgtacagg aaatttcttc gccgcgcgag gtgaccaccg agcggactgt attgtcgccg
     5761 cggatttcgg agagtgtcgt accgagttgc aggtccacat gctttcccac ttcgttcgtg
     5821 accatttcag cctcttcgcg gggcagaata tgcgaaaagt agagtggctc acgcaccagc
     5881 atcgtcaccg gtattctgcg gctgccgagc atttcagcga gttcgacgcc gataagcccg
     5941 ccacccgcga tcactgcgct cgcgaccggg gtcctggtct gttcttcgag ccggtcgaga
     6001 tcggcttttg aatagagtcc ctggacgcct ggcaggtttt cacccgcgag acccggctgg
     6061 cgcgggcgcg agccgggagc aagcagcagg taatcatagc tgtatgtcgc tgattcaccc
     6121 gtgagtcggc gcgactgcag gtcgagcgag gtgaccaggt cctgctgcag gttaatgcgt
     6181 tgcttgcgat aaaaatccgc ggggtgtacc acggtatctg caaactgcag ctgccgcatg
     6241 tagatataca tcagcgcggt gcgtgaataa aacagcggag tttcttcgga gatcagggtg
     6301 atctctgcct ctggggccag ctgccgcaac ctgagcgctg ccgttgtgcc cgcgatgccg
     6361 ttaccgacaa tgacaacctt aactgacaac tgcccccccg cagcttgagc cagaacccgc
     6421 cgtacagcca taacaatggt ttgctgtgcg gatactgcgc gacgaaaacc gttcgggcgt
     6481 aaaatcgtcg atgtgcgcta ctgcctcagc gggcatatcg agcatctggt taaaatcaca
     6541 gtcatagacc tgtccgtcgt agccgaccga tatctgcgtg cggcacatta ggcccggtat
     6601 tgtctgtgga ttgaacgcat tgaccagggt cgtgaggtag gtagcggttt tacccgcccg
     6661 ctccagcgca aacaggaacc ggttgattgg catgttgttg atgcagtaga gactgttgaa
     6721 ggtgatgccg tggtctgcca tgagcttctc acgaaactcg cgctcgagct gtagctggtt
     6781 actgctcaag aataccccgc tcggattata gaccaggttc agtttgagct gtgcaccgta
     6841 accgagcgcg ttgagttttt tcaggcccgc aatggacttc tggaaaaccc cggctccgcg
     6901 ctgcgaatcg gtgcgctgtt cggcgaagtg gggcagcgaa gcaacgatct cgacttcatt
     6961 cgccgcaaga aaatccgcca ggtcttcgtg tcccggttcg gagagaattg tcaggttgca
     7021 gcggtcgatc aggtgtttac c
//
LOCUS       DAOQJF010000160         7040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5931_length_7040_cov_5.116535, whole genome shotgun sequence.
ACCESSION   DAOQJF010000160 DAOQJF010000000
VERSION     DAOQJF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5931_length_7040_cov_5.116535"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PLF85_09480"
     CDS             complement(<1..1144)
                     /locus_tag="PLF85_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNE19939.1"
                     /translation="MKRTLALICCLTAAIVLPAVAQAATLNDAAKDVSAFFAKNPGKT
                     VAVLAFKHGDAKAIAEVTGVQDRLTEQLVAAGKVTVIERDRIEQVLREHQIDQSGATD
                     KARIGQLLQADFLLTGSITPGKRGQIEISARLVEVTSLKIVATARATADRSDFGATMV
                     TRPRGNYLGEPLVQIAILVDTSSSMDGLIDQARTQIWKIVNTLANGNREGKKPRLEVA
                     LYEYGNSGLDASQNYIRQVLPFTASLDKVSEKLFELKTNGGEEYCGAVIARALAELEW
                     KKYDDVYRVVFIAGNEPFTQGPVDFRASMDQARAQGVFVNTIFCGNRQEGIATQWLTG
                     AQLAQGDFHVINQDRMVQVVATPYDEEIQRLGAEYNATVIPMGNAGR"
     gene            complement(1269..1544)
                     /locus_tag="PLF85_09485"
     CDS             complement(1269..1544)
                     /locus_tag="PLF85_09485"
                     /inference="COORDINATES: protein motif:HMM:NF021165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19940.1"
                     /translation="MRKALLFLLACLAPIVATDDAKTLFEAKCATCHALPDPESLGEK
                     QWQAVLKTMQKRMREVGKPELTADEFAKIHCYLTRQNSSACSVAENR"
     gene            complement(1541..2035)
                     /locus_tag="PLF85_09490"
     CDS             complement(1541..2035)
                     /locus_tag="PLF85_09490"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNE19941.1"
                     /translation="MALWSIHCSNLLPDSRDLRGQNPGNILAVGQAAPDFALPVTDGS
                     TLTLASGLSGKNGLVIYFTMWCPVCTSHTDEVVQAIMPAYPDARYVMVDYVSSGIGEA
                     ATLKTYGGYDSTGIVVALDKSQSFMNSYGATMAYVVIIDSTGKIRMNEVYKRQKMEQI
                     LGSL"
     gene            complement(2073..3050)
                     /locus_tag="PLF85_09495"
     CDS             complement(2073..3050)
                     /locus_tag="PLF85_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19942.1"
                     /translation="MRKVIFGMALAIPMILLLSPAQPVYAGSCCGGGTAGALLMPKSA
                     KWLFAGSGDFEKYNGYFDRGGKYLSDPPNSDLRQYRLNGGVALRLASRWQAAFASGYV
                     FNSNKYSGLVSNTNGPADSVTSLTYETFDNIRCVWKVRKWADMVPAVYLASQLTLPTG
                     VSPYDNVQSSFDITGRGFYRLEGKAIVEKTVYPFTLALTVLYGTHFARPVNRDYGLYV
                     EPYRKRLGDRFTGTAALSYTFFTESMASLTVSTTYTYLYEADATINGYVDKTTGFSRH
                     LFGATLAWATPERELIYTLSYNPALREPGLQQNFPATHVVSIGVNYVIR"
     gene            complement(3056..4150)
                     /locus_tag="PLF85_09500"
     CDS             complement(3056..4150)
                     /locus_tag="PLF85_09500"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNE19943.1"
                     /translation="MQTQKIKQIRAAMSAAIVAFAVNACVQNEDAAAAAAVTAPSGLS
                     YGAKTITLAQNVAVSKSASVTGSVASCSVAPALPPGLALDSTTCSISGTPTTTQAATS
                     YTVTAANSAGSTTASVKIAVVQYTATMQGTPTHSVGKTTFQIKVTDSTGNGVAGLNPK
                     IVPEMDMGSMVHTTPVDTVTDNGSGLYTAVVYYLMSSDGGTWYIKTLNSGGMTEIGTR
                     IAMTVGGSSTARVSVSNSADQYSNMMTATNRPYFVFVDAVSGGAGNHTVSIFAATRKS
                     MMDHPALANGLVLTPATGSDWTVNGATTSIRLSIDGGTSWVGTLSDNGTGHFSLGSIA
                     GLTAGSKTLTFEVTVNGTVRGTGSLTAVIP"
     gene            complement(4153..4635)
                     /locus_tag="PLF85_09505"
     CDS             complement(4153..4635)
                     /locus_tag="PLF85_09505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE19944.1"
                     /translation="MRLAFSYMVLAILGLVHSSWPAEKVTLRDLPLLGKSARPLTGYA
                     GKPHVLVFWAVWCGPCAAELRELERVSRSEKTPFIGIAVDSVEKQAARMVERTGVTFA
                     NYLDNDSAFADALGVSGVPSLLVIDSKGNVVWSQSGYSVFPEQALKEQIKKVSNNKKE
                     "
     gene            complement(4861..5253)
                     /locus_tag="PLF85_09510"
     CDS             complement(4861..5253)
                     /locus_tag="PLF85_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19945.1"
                     /translation="MRLILRFRFLHLAGLGFFALQLADAPAIVADAMTPKLRICPHTK
                     AQYKRGNPCPCGHKHGKNRDLPRFVSEHGDCDSDADSERLRAPGFEAFVFTYDSEYLL
                     SRELMAIVHPSVLFLPSGLTVDPPDTPS"
     gene            complement(5348..5989)
                     /locus_tag="PLF85_09515"
     CDS             complement(5348..5989)
                     /locus_tag="PLF85_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNE19946.1"
                     /translation="MVDQEYLKPTWTIDSDHPEVVAYAQKITQGLTTPREKAVALYYA
                     VRDLVMYDMMVEHFSREVFKASHCLHAKRTFCIPKAVLLAACARAVGIPSRLGFADVT
                     NHLATPKMLEMLGGDYFAFHGYTDLYIDGKWVKATPAFNERLCRFFKIKPLDFDGRSD
                     SIFHEFSADGKKHMEYIHNHGTFADMPYELMIETFHKHYPHWFAPGGSLESWA"
     gene            complement(5982..6512)
                     /locus_tag="PLF85_09520"
     CDS             complement(5982..6512)
                     /locus_tag="PLF85_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNE19947.1"
                     /translation="MIRDGASQLNWLNAGAVCLNFNLRRTSRAVTQYYDEALKPTGVK
                     VTQFTILMAIASQSTGAGRAAGRSSQDGQAAAQGAAGGSITDIARLAMVDRTTLTRSL
                     KILEDDKLIEIRKAQPGNKRRVIITAKGIKKMKEIYPYWFRAQRELVKKLTPVKTREL
                     RHLLGDIKNSAEVLHG"
     gene            6582..6953
                     /locus_tag="PLF85_09525"
     CDS             6582..6953
                     /locus_tag="PLF85_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19948.1"
                     /translation="MEKNCTIGGTWTSQSKKKGNSLWKVKLVWNNGEGNSGYGFSIKS
                     NGAHKDTFGSFTVDGSCSEGFCDFHQNYTSGKFKGRYYSYRAGYSELKPLNLSGDYGL
                     VEGEQQVKEGKFTLNKLTCNK"
BASE COUNT         1530 a         1979 c         1942 g         1589 t
ORIGIN      
        1 gcctgcccgc attgcccatg ggaatcactg ttgcgttgta ttctgcaccc agacgctgta
       61 tctcttcgtc ataaggcgtt gcaacaacct gaaccatacg gtcctggttg atcacgtgaa
      121 aatcaccctg cgcaagttgc gctcccgtga gccactgcgt ggcgatgcct tcctgccggt
      181 taccgcagaa gatggtattc acgaatacac cctgcgcgcg tgcctggtcc atgctcgcac
      241 gaaaatcgac cggcccctgc gtgaaaggtt cgttacccgc aatgaacacg acgcggtaga
      301 cgtcgtcgta ctttttccat tcgagctcgg cgagcgcacg cgcaatcaca gcaccgcagt
      361 attcttcgcc accgttggtc ttgagctcga acaatttctc tgaaaccttg tcgaggctcg
      421 cggtaaacgg cagcacctgg cggatgtaat tctgtgacgc gtcgaggccg ctgttgccgt
      481 attcatagag cgcgacttca agacgcggtt tcttaccctc acggttgcca tttgccagcg
      541 tattcactat tttccagatc tgcgtgcgcg cctggtcgat caggccatcc atgctgctcg
      601 aggtatcaac taaaatcgcg atctgcacca agggttcgcc gaggtagttg ccgcgcggtc
      661 tggtgaccat ggttgcacca aaatcagacc ggtcggcggt tgcccgcgcc gtggcgacaa
      721 tctttaagct cgtgacctca accaggcgcg cagaaatttc gatctggccg cgtttgcccg
      781 gtgtaatgct gccggtgagg agaaaatccg cctgcagtaa ttgccctatg cgggccttgt
      841 cagtcgcgcc cgattggtcg atctggtgct cgcgcaagac ctgctcgata cgatcgcgct
      901 caattacggt gactttaccc gcagcaacga gctgttctgt cagccggtct tgcacaccgg
      961 tcacttcagc gatggctttg gcatcgccgt gcttaaaggc gagtaccgcg accgtcttgc
     1021 cgggattttt cgcaaagaag gcagagacat cttttgcggc atcattgagc gtggcagcct
     1081 gtgcgacggc aggcaagacg atcgctgcag tgagacaaca gatcagggcg agcgttcttt
     1141 tcataggctt tagacttcta aaggggctaa agtgttcgga aaatttttga cgatgatgga
     1201 agagctggaa accacggcga cacggcgggt acggccgact ggaaaggatc gcactggctt
     1261 aacgcacctt accgattttc tgccacacta caggcagagc tgttttgcct cgtaagatag
     1321 cagtggattt ttgcaaattc gtcagcggtc agttcgggct tacccacttc gcgcatacgc
     1381 ttctgcatgg tcttgagcac tgcctgccat tgtttttcac ccaggctctc ggggtctggc
     1441 agagcatggc aggtcgcaca tttggcttcg aataaggtct tcgcgtcgtc ggtcgctaca
     1501 atgggcgcaa ggcaagcgag aagaaacagc agcgcctttc tcataacgac cccagaatct
     1561 gttccatctt ctgcctcttg tagacttcat tcatgcggat ttttcccgta ctgtcgatga
     1621 tcaccacata ggccatcgtc gcaccgtagc tgttcataaa actttgtgac ttgtcgagtg
     1681 cgacgacaat gccggtagaa tcgtaaccgc cgtaggtctt gagcgtcgcc gcttcaccaa
     1741 tgcccgacga aacgtaatca accatcacat agcgggcatc ggggtatgcc ggcatgattg
     1801 cctgcacgac ctcgtccgta tggctcgtgc aaacggggca ccacatcgtg aagtagatta
     1861 ccaggccatt cttaccagaa agccccgagg ccagcgtcaa tgtactgcca tcggttacgg
     1921 gcagtgcaaa atcgggcgca gcctgcccga ctgcgagaat gttaccgggg ttctggcccc
     1981 gtaaatccct ggaatcgggc aaaaggttcg agcaatggat gctccacaaa gccattaaca
     2041 gcacagggta aatcttcctc agccttttta atttagcgaa taacatagtt cactcctatt
     2101 gaaacgacat gcgtcgccgg gaaattttgc tgcagcccag gttctcgcag tgcagggttg
     2161 tagctcaacg tatagatcaa ctcgcgttcg ggtgttgccc atgcaagcgt cgcaccgaac
     2221 aagtgccgcg aaaaccccgt agtcttatcg acgtagccat tgatggttgc atcggcttcg
     2281 tacagataag tgtacgtcgt actcacagtc agacttgcca tcgattcagt aaaaaaggtg
     2341 tagctgagcg ccgcggtacc tgtgaagcga tcgccgagcc gcttgcggta cggttcaacg
     2401 tatagcccat agtcgcggtt taccggccgc gcaaagtgcg tgccgtaaag caccgtcagg
     2461 gcgagagtaa acgggtagac ggttttttcg acaattgcct taccctcgag acggtaaaag
     2521 ccacgacccg tgatgtcgaa actcgactgc acattgtcgt acggtgagac gccggtgggg
     2581 agcgtcagtt gcgaagcaag atagaccgcg ggaaccatat cggcccactt acgcaccttc
     2641 cacacgcaac ggatgttatc gaacgtctcg tacgtcagcg atgtaacgct atcggccggc
     2701 ccgttcgtgt tcgaaaccag gccactgtat ttgttcgaat tgaacacgta gcccgaagcg
     2761 aacgcggcct gccagcgcga agcaagacgc aaagcaacac cgccgttcaa ccggtactgc
     2821 cgcaggtctg aatttggcgg gtcgctcaga tacttaccgc cgcggtcgaa gtagccgttg
     2881 tacttctcaa aatctccgct gccagcaaag agccacttcg ccgacttggg catgagcagt
     2941 gcgccggccg tgccgccccc gcagcacgag ccggcgtaaa ccggctgtgc tggtgagagc
     3001 aaaagaatca tcggtatggc caaagccata ccgaaaatta cctttctcat gtgacctacg
     3061 gaatcacagc cgtcagtgag ccagtgcccc tgacagtgcc attcacggta acctcaaagg
     3121 tcagcgtttt cgaaccggcc gtcagccctg caatactgcc cagcgaaaag tgtccggtgc
     3181 cattatcgct gagcgtaccg acccaactag ttccgccgtc gatcgagagc ctgatgctgg
     3241 tcgtcgcccc gttcaccgtc cagtctgatc ccgttgccgg agtgagtacc agtccgttcg
     3301 caagcgcagg gtgatccatc atcgatttgc gcgtggcggc aaagatactc accgtatggt
     3361 tgcccgcgcc tcctgagacc gcatcgacaa acacaaagta cgggcggttg gtggcggtca
     3421 tcatgttgct gtactggtct gcgctgttgc tcaccgaaac acgcgccgtc gacgatccac
     3481 cgactgtcat cgcaatgcgc gtgcctatct ctgtcatacc gccgctgttc agggtcttga
     3541 tataccatgt tcccccgtcg gagctcatca ggtaatagac cactgccgtg tagagacctg
     3601 atccgttgtc ggtcaccgtg tctaccggtg tggtgtgtac catcgagccc atatccatct
     3661 ctgggacgat tttgggattc agccccgcaa cgccgttgcc cgtactgtcg gtcaccttga
     3721 tctgaaaagt ggtcttaccc acactgtgcg tgggtgtccc ctgcatagtc gcggtgtact
     3781 gtaccacggc aattttcact gaagcagtcg tcgaaccggc gctgtttgcc gcagtaaccg
     3841 tgtacgatgt cgccgcctgg gttgtcgtcg gtgtgccgct gatgctgcag gttgtgctgt
     3901 cgagagcaag ccccggcggt agcgccggcg cgacgctgca cgatgccaca ctaccggtga
     3961 cgctggcact ctttgatacg gcaacattct gcgcgagggt gatggttttt gcaccataac
     4021 tcagtccact gggtgctgtc accgcagcgg cggcagcggc atcttcattc tgcacgcacg
     4081 cgttgactgc gaacgccacg atcgctgccg acatggcagc gcgaatctgt tttatttttt
     4141 gagtttgcat aattattcct ttttgttgtt acttactttt ttgatctgct ctttcagagc
     4201 ctgttccgga aaaaccgagt agccgctctg cgaccagacg acgttgccct tactgtcgat
     4261 cacgagcagg gagggcacac cgctgacgcc aagcgcatcg gcgaaagcag aatcgttgtc
     4321 gaggtagttg gcgaaggtga cgccggttct ttcgaccatg cgcgccgcct gtttttcgac
     4381 actgtcgact gcaatgccga taaagggagt tttctctgaa cgcgaaacac gttcgagctc
     4441 gcgcagctct gccgcgcacg ggccacacca taccgcccag aaaaccagca cgtgcggttt
     4501 acccgcgtaa ccagtcagcg gtcgtgccga tttgcccagc agaggcagat ctcgcagcgt
     4561 aactttttca gctggccatg agctgtgcac taagccgaga atagccagaa ccatgtatga
     4621 gaatgctaag cgcatactcc cccttggata cacgaggtat cgggaagacg gcggacacaa
     4681 tgccctctat tgccgtcccc ttagaatttt tttgcttaag tgttttgccg gcaagtgtag
     4741 cgacgccgta cgcccattca gacgcaggtc aatcaggaca cgtttacaaa acagtcaggg
     4801 caacaggcgc agaaatgaat tcaagccgcc tgagggtgcc gccggtggca ctggcagtta
     4861 ctatgaagga gtgtcgggag ggtcaacggt aagaccggac gggagaaata gtacagaagg
     4921 atgcactatc gccattagct cgcgcgaaag caggtattcg ctgtcgtagg tgaagacaaa
     4981 tgcttcaaag cccggcgcgc gcaggcgttc tgaatcggca tcactgtcgc agtcaccgtg
     5041 ttcgctcacg aaacggggta agtcgcggtt cttgccatgc ttgtggccgc aagggcacgg
     5101 attgccccgt ttatactgcg ctttggtgtg cgggcaaatg cgcaatttgg gagtcatcgc
     5161 atcggcaacg atcgccggcg cgtcggcgag ctgcagcgca aagaagccca agcccgccag
     5221 atggagaaat ctgaacctga ggatcaggcg cacggttcaa cgccgcagag tgtcttgccg
     5281 cgtaaatcga tttaactacc tttttctacg gcttgtcatt ggtgtaaagg caagatcgat
     5341 ctgtgactca cgcccagctc tccagcgaac caccgggtgc aaaccagtgc gggtagtgct
     5401 tatgaaaggt ttcgatcatg agctcgtacg gcatgtcagc aaaagtgccg tggttgtgga
     5461 tgtactccat atgcttcttg ccgtcggctg aaaactcgtg gaagatcgaa tcgctgcgcc
     5521 cgtcaaaatc gagtggtttg atcttaaaaa aacggcacag ccgctcattg aaggccggcg
     5581 tcgccttcac ccacttgcca tcgatataaa ggtcggtata gccatgaaag gcgaagtaat
     5641 cgcctccgag catctcgagc atttttggtg tcgcgaggtg gttcgtgacg tcggcgaatc
     5701 ccagacgcga gggtatgccc accgcccgtg cgcacgccgc cagcagcacc gctttcggaa
     5761 tacaaaatgt gcgcttcgcg tgcaggcagt ggcttgcctt gaatacctcg cgtgagaaat
     5821 gttcgaccat catatcatac atcacgagat cgcgcacggc gtaatagagt gcgacggctt
     5881 tctcgcgtgg tgtcgttagg ccctgtgtaa tcttctgcgc ataggcaaca acttccgggt
     5941 gatccgaatc gattgtccag gtcggcttca ggtattcctg gtcaaccatg cagcacctcg
     6001 gcggagttct tgatgtcacc gagcaggtgc cgaagctcgc gggtttttac cggcgtaagc
     6061 ttcttcacca gctcgcgctg tgcgcgaaac caataggggt aaatttcttt catctttttt
     6121 atgcccttgg ctgtgataat cacccgccgt ttgttgcccg gctgcgcttt gcgaatctct
     6181 atcagcttgt cgtcttcgag aatcttgaga ctgcgcgtga gggttgtgcg atcgaccatg
     6241 gcgaggcgtg caatgtcggt gatggagccg ccggccgccc cctgggccgc agcctggccg
     6301 tcctggctgc tgcgacctgc ggcacgtcct gcgcctgtgg actgcgaggc gattgccatt
     6361 aatatagtga attgcgtcac cttcacgccc gtcggtttca gggcttcgtc gtaatactgg
     6421 gtcactgcgc gcgaggtgcg gcgcagattg aagttgagac agactgcacc agcgttgagc
     6481 cagtttaact gtgaagcacc atcgcggatc atttagctac ataaatatag tgcatataca
     6541 cagtcaagtg ggttcgatca tacgaccgga cacgccagca ggtggaaaaa aattgcacga
     6601 tcggcggcac ctggacgagc caatcaaaaa agaaaggtaa ttcgctttgg aaagtcaagc
     6661 tggtctggaa caacggcgaa ggcaacagcg gatacggctt ttccataaaa agcaacggcg
     6721 cgcacaaaga cacctttggc agtttcaccg tcgatggcag ctgctctgaa ggattctgtg
     6781 acttccacca gaactatacc tcgggcaagt tcaaaggccg ctattattcg taccgcgccg
     6841 gatactcaga gctcaagcct cttaacctga gcggcgatta tggcctggtt gaaggggaac
     6901 aacaggttaa agagggcaag tttaccctga acaagctgac ttgtaacaaa taaacgctgc
     6961 ggcgcctctc cacctcgtgt gcctggtgcg cacatcgact gacatcgtgt caggtgcgca
     7021 ctctggcacc tcgtgtgcct
//
LOCUS       DAOQJF010000161         7040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5932_length_7040_cov_4.718540, whole genome shotgun sequence.
ACCESSION   DAOQJF010000161 DAOQJF010000000
VERSION     DAOQJF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_7040_cov_4.718540"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLF85_09530"
     CDS             <1..414
                     /locus_tag="PLF85_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNE19949.1"
                     /translation="TNPLIGTHDAQWGERFVDQSNSYTPELRKVAREVGQQLQVPLHE
                     GVYAGLTGPAYETPAEIRMLRTLGADACGMSTVPEAIVARQAGMRILGISLITNMGAG
                     IAAPLHHSEVVEVAEQRKKDFENLLKGIIKRINEE"
     gene            539..1843
                     /gene="nuoF"
                     /locus_tag="PLF85_09535"
     CDS             539..1843
                     /gene="nuoF"
                     /locus_tag="PLF85_09535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNE19950.1"
                     /translation="MHPAKARAPHSKELRVVLSYFHKENSHTLKVYEAEGGYQQLKRL
                     LVTEKEQWNPNAIIEEVKKSNLRGRGGAGFATGMKWSFVPKDGPEPKYVVCNADESEP
                     GTFKDRFMLEEIPHSIIEGMIIAALALNSEQGYIYIRGEFYKGWERIEAALAEAYAAG
                     YLGDNILGTGRKFHLTTYRGAGAYICGEETALLESLEGKRGHPRLKPPFPAFKGLYAS
                     PTVVNNVETYATVPAVFRLGAEAYGKLCKGSEKSGGTKVFSISGHVQNPGIYEIPLGL
                     PLKEVIEIAGGLRPGRTLKAVIPGGSSTPILTSEDVEKAVLDYEGMAAVGTFLGSGGL
                     MILDDTTDMVELTHRVAHFYGHESCGQCTPCREGSRWVSDVVHRVRDRHAEPGDLDTL
                     LSFAGNMGNGTTICPFSDAVVMGVTPIVKKFRGDFVAHLQAK"
     gene            1851..3683
                     /locus_tag="PLF85_09540"
     CDS             1851..3683
                     /locus_tag="PLF85_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE19951.1"
                     /translation="MLGKIRGLYENTFTTIRAKLIGIISLIILVSMLLFTGISLNLFQ
                     SNMSELVKLNNGNNAKMLSDKVESELRSQSDALRLLATLSREGSTAMNAYFKDSSDFL
                     WVGDYPALNASPSRTFFRAAKLERLNLTQEGVEKLVRFTPEDASAIRQGGTIIRAAQS
                     PRQHIWLLAIPFAEKGSERALAAVMNGDALTALFAAVKSKGTSSSLYAAMLVDAQFRL
                     VAHSDNRKYKPAQDMKQHPAVEIAFKRFAQNVVTGVQRYGIGETFEYGSFRRLALGGA
                     ALITTVNEDIAFEAVRWARITTILIALIIMTIAILFIYFFSRTISDPLKRLVGATQRI
                     RAGHYDTHVDNASRDEVGELTHAFNEMTDGLREREHLKGAFGKFVNEEVAKMVMKGDL
                     ALGGESKIATVFFSDIRSFTSISEKMTPAEVVGFLNEYMTLMVDIVHKYGGVVDKFIG
                     DAIMAIWGAPVSKPNDVENAVNAALEMRAVLHKFNQGRGSRTKPIIRIGMGLNTGEVL
                     AGQIGSNDRLEYTVIGDTVNLASRMEGLNKTFGTDILITQNTYELVKKKFVCQPLHKV
                     QVKGKAEAQRVYAVMGKTGDAKAPKSLAELHKFTGLYPQKKKGK"
     gene            3686..5254
                     /locus_tag="PLF85_09545"
     CDS             3686..5254
                     /locus_tag="PLF85_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE19952.1"
                     /translation="MVLSRRDKAVTAILVAIILILLYIMQCHWRNRFGARGDAKPVGE
                     VEYKYHRVQRKYSDRMLWEEVEPKSPVYAYDWVMTKEKSDARITLKNGMKVDMDPESM
                     VEIDETHDGVGLTLRDGTIRADTRDAKSGTITAADGTRIDLEKGEAQISTDGQAVSVD
                     VKEGKATLRNGDRETKAQAGEVINKTANGVTKGKTAITLKSPQQEIVLDDPERPVDFA
                     FEAPQGSDDCKIHLNAGNKTERVVRVKAGTTHAERLGDGTYHWRATCQAAGEPVSSGT
                     GVFRMRPAAPFAIVAPQTGETVTSNSAETLALRWRSEGRVKVELADNPTFATVLKTAE
                     SGSGSYPVPNLKPGKYYWRVTALSEGNRSLQSEFSVSEKGELLTEADPIKPVETGKAS
                     STVAGTEKPKENKEPPPKQMRISATAPGSVTIEPDASKAAVRINWTPVNAQIRYRLAV
                     STDEYFSEEYVAKQVSGKGSAVVNLPPGTYYYRVRAAGKSGGRALAVSQARKVEIRRK
                     KLPPPPRVKSVQAE"
     gene            5331..6107
                     /locus_tag="PLF85_09550"
     CDS             5331..6107
                     /locus_tag="PLF85_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19953.1"
                     /translation="MKALWMILLAGLITAGCGRGYEELLQQGTEQRRAQKWAQAKHTL
                     FAAVEKKQTPEVYKELGNVFLLGEQNIAEAEGYYRKALAIDANYINAQFNMAVISLKK
                     YELTLDDKGKGDDALLKEAEGWFNKVYSQNPNFAVGIEEMAKYHYYKHDYAKALETAQ
                     KAVAVDNRNANAYSVIGQIYLSGLKDYKQAFENFEQARSLNNKDADVVYFLWATSEKL
                     RKAQDAKQFKTRYEQMLKQEGLSAEQAKERIRRLENQLKS"
     gene            6150..>7040
                     /locus_tag="PLF85_09555"
     CDS             6150..>7040
                     /locus_tag="PLF85_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19954.1"
                     /translation="MLEKSLKKAGKDREKPPQTGEKRSVGWLRTSLGERTSALADKID
                     LEEQYARLESLAGAVPEPPKPSLRDRARRLLGNIVPAAQPGNAQPATEQAPAGAGRPH
                     MTLRQRAALLMKRLSGQGGNQGGAEGSGAPPAAGSTGATGQHDTHLAPDDAVDHELPP
                     PAFFPWLALSDEGTLHSHVSFGDDHGHLPEPAETLVAAQPQELEQSKTPLAEFADTIG
                     RITENLKNLHVQLDQGNFIRGVQVEQQGRPVAEPQTAQTPIISHHVPVDSTLPAAGVE
                     IQGQPVPQAAAEPKDVFWPRN"
BASE COUNT         1740 a         1927 c         2023 g         1350 t
ORIGIN      
        1 accaacccgc tgattggtac gcatgatgcg cagtggggtg aacgttttgt cgaccagagt
       61 aacagttaca ctcctgaatt gcgcaaggtt gcgcgcgaag tggggcagca gttgcaggtc
      121 ccgttgcatg aaggtgttta cgccgggctt acagggccgg cgtatgaaac ccctgcggag
      181 atacgcatgc tgcgcacgct cggtgcagac gcctgtggta tgagtaccgt gcccgaagcc
      241 atcgtcgccc ggcaggcagg catgcgcatt ctcggtatca gcctgatcac caacatgggt
      301 gcgggcatcg ctgccccgtt gcaccacagt gaagtggttg aggtggccga acagcgcaag
      361 aaagattttg agaacctgct caagggcatc atcaaacgaa tcaacgaaga atagggtcgc
      421 acgcaccagg ttgggtgctc ctgcaggccg ccggattttg gcatcaactc acccggagag
      481 gtaattcacc gcccgtcttt ttctttacgc cccttatccg ctttgcctga tgctctcaat
      541 gcatcctgcc aaagcgcgag cgccgcacag taaagaactg cgtgttgttc tcagttattt
      601 ccacaaagaa aactctcaca cgctgaaggt ttatgaggcc gaaggcgggt accagcagct
      661 caaacgtctt ctggtgaccg aaaaagagca gtggaacccc aacgcgatta ttgaagaagt
      721 aaagaagtcc aacctgcgcg gccgtggtgg tgcaggcttt gccactggca tgaagtggtc
      781 atttgtaccg aaagacgggc ccgagcccaa gtatgttgtc tgtaacgccg atgaatcaga
      841 gcccggtaca ttcaaagacc gtttcatgct cgaagagatt ccgcattcga tcatcgaagg
      901 aatgatcatt gcggcgctgg cgcttaacag cgagcagggt tacatctata tccgcggcga
      961 gttttacaaa ggttgggagc gtatcgaagc agcgctggct gaagcgtatg cggctggata
     1021 cctcggtgac aacattcttg gcaccgggcg caaatttcac ctcaccacgt accgcggggc
     1081 aggtgcttac atctgcggcg aagaaacagc gctgctcgaa agccttgaag gcaaacgcgg
     1141 ccacccgcga ctgaagccgc cgtttccggc gttcaagggc ctctatgcat cgccgacagt
     1201 tgtcaacaac gtcgaaacct acgcgacagt cccggcggtt tttcgcctcg gcgcagaagc
     1261 ctacggtaaa ctctgcaagg gcagcgaaaa atccggaggt accaaggtat tttctatttc
     1321 tggtcatgta cagaaccccg gcatctatga aattccgctt gggctgccgc tcaaagaagt
     1381 gatcgaaatt gccggcggcc ttaggccggg gcgtacgctc aaggcagtga ttcccggcgg
     1441 cagctcaaca ccgatcctga cttctgaaga cgtcgagaag gcagtgcttg attacgaggg
     1501 tatggcagca gtgggtacat tcctcggttc cggtggactg atgatactgg atgatacaac
     1561 cgacatggtg gagctcacgc accgcgtggc ccacttctac ggccacgaaa gctgcgggca
     1621 gtgtacaccg tgccgcgaag gatcgcgctg ggtgtctgat gtcgtgcacc gcgtacgcga
     1681 ccggcatgcc gaacctggcg atctcgatac gctgctgagt ttcgcaggca acatgggtaa
     1741 cggtacaaca atctgcccgt tctccgacgc agttgtcatg ggcgtgacac cgatcgttaa
     1801 aaaattccgt ggcgacttcg tcgcgcacct gcaggcaaaa taaggaaaat atgttaggga
     1861 aaatccgcgg cctctacgag aacaccttca ccacgatcag ggcaaagctg attggtatta
     1921 tttccctgat tatcctggtg agcatgctgc tctttacggg catctcgctg aaccttttcc
     1981 agagtaacat gtccgagctg gtgaagctca acaacggcaa caatgccaag atgctttcgg
     2041 acaaagtgga gtcagaactc cgttcgcagt ccgatgcgct gcgcctgctg gcgacgcttt
     2101 cccgcgaggg ttcgaccgcg atgaatgcct attttaaaga ttcgagcgat tttctctggg
     2161 taggagatta cccagcgctc aacgcttcac cttcacgtac tttctttcgc gcagcaaaac
     2221 tcgagcgcct caatctcaca caagaaggtg ttgagaagct cgtgcgtttt acgcccgaag
     2281 acgccagcgc gatacggcag ggcggaacga tcatccgcgc ggcccaatcg ccgcgccagc
     2341 acatctggtt gctggcaatc ccttttgctg aaaaaggcag cgagcgcgcg ctggcagcgg
     2401 tcatgaacgg tgatgcgctc accgcgctct ttgctgctgt taaatcaaag ggcacatcga
     2461 gttcgctcta cgcggcgatg ctggtcgatg cgcaattcag gcttgtggct cattcggata
     2521 accgcaagta taagccagcg caggacatga agcagcatcc ggcagtggaa atagcattca
     2581 agcgatttgc acagaatgtc gtgacgggcg tgcagcggta tggcattggc gaaacttttg
     2641 aatatggtag cttcaggcga ctggcgctcg gtggcgcggc gctcattacc accgtaaacg
     2701 aagatattgc gtttgaggca gtccggtggg cgcgtatcac gacaatcctg atcgcactga
     2761 ttatcatgac catcgcgatt cttttcatct acttcttctc acgcacaatt tccgatccgc
     2821 tcaagcgcct ggtcggtgcc acgcagcgaa ttcgtgcggg tcattacgat acccacgtag
     2881 acaatgcttc gcgcgacgaa gtaggcgaac tcacgcatgc ctttaacgag atgaccgacg
     2941 gcctgcgcga acgggagcac ctcaagggcg ctttcggcaa gttcgtgaac gaagaagtgg
     3001 ctaagatggt catgaagggt gatctcgcgc tcggcggtga atcgaagatt gccaccgttt
     3061 tcttcagtga catacgctcg tttacgtcta tctctgaaaa gatgacgccg gcagaggtag
     3121 tcgggtttct caatgagtac atgactctca tggtcgatat tgtccacaag tacggcggtg
     3181 tagtagataa gttcatcggc gacgctatta tggcaatctg gggagctccg gtgtcgaagc
     3241 cgaatgatgt cgaaaacgcg gtcaacgccg cgctcgaaat gcgtgccgta ctgcacaaat
     3301 tcaaccaggg caggggtagc cgcactaaac cgatcatccg tatcggtatg gggctcaata
     3361 ccggcgaggt gcttgccggg caaatcggca gcaatgaccg gttggagtac acggtgatcg
     3421 gcgacacggt gaaccttgcc tcgcggatgg aaggcctgaa caaaacattt ggtacggata
     3481 tcctcatcac gcagaatacg tacgagctgg tgaaaaagaa atttgtctgc cagccactgc
     3541 acaaggtgca ggtaaaaggt aaggcagagg cgcaacgcgt atacgccgtc atgggcaaga
     3601 ccggtgatgc aaaagcgccg aaatctctgg cagagctgca caagtttacc ggtctctacc
     3661 cgcagaagaa aaagggtaaa taactatggt tctcagccgc cgcgacaaag ccgtcaccgc
     3721 catactggtg gccattatcc ttattctgct ctacatcatg cagtgccact ggcgcaaccg
     3781 cttcggtgcg cgcggcgacg caaagcccgt gggcgaagtc gaatacaagt accaccgcgt
     3841 gcagcgcaag tactctgacc gcatgctgtg ggaagaagtt gagccaaaat ctcccgttta
     3901 tgcatacgac tgggtgatga ccaaagaaaa atccgacgca cgcatcacac tcaagaatgg
     3961 aatgaaagtc gatatggacc cggagagtat ggtcgaaatc gatgaaacgc acgacggtgt
     4021 gggcttgacc ctgcgggatg gtaccattcg cgcggacact cgcgatgcga aatccggtac
     4081 tattacagcg gctgatggca cacgtatcga cctggaaaag ggcgaagcgc agatttctac
     4141 cgatggccag gccgtgtccg ttgacgttaa agaaggtaag gcgactctgc gcaacggcga
     4201 ccgcgaaacg aaagcacagg ccggcgaggt gatcaataaa accgcgaacg gcgtcacgaa
     4261 gggcaagacc gccattaccc tcaagtcacc gcagcaggaa atcgttctcg atgatcccga
     4321 gcggccggtt gactttgctt tcgaggcccc gcagggtagc gatgactgca agatacacct
     4381 gaacgccggg aacaaaacag aacgcgtcgt acgggtcaaa gcgggcacga cgcacgctga
     4441 gagactcggt gatggtacat atcattggcg cgcaacctgc caggcggcgg gcgaaccggt
     4501 ttcttccggt acgggtgtgt tccgtatgcg gcccgccgct ccctttgcca ttgttgctcc
     4561 acaaacgggt gaaacagtga cgtcgaacag cgcagagacc cttgcgctgc gctggcgctc
     4621 agaaggcaga gtgaaggtcg aattggcgga taacccaacg tttgcgacgg tcttgaagac
     4681 cgctgaaagc ggctctggtt catacccggt gccaaacctg aagcctggca aatactattg
     4741 gcgtgtgacg gcgctcagcg agggcaatcg tagcctgcag tcagagtttt cagtatcaga
     4801 aaagggcgag cttctgacgg aggctgaccc aataaagcct gttgagacag gcaaggctag
     4861 ctcaactgta gcgggcacgg aaaaacccaa agaaaacaag gaaccgccgc cgaagcaaat
     4921 gcgtattagt gcgaccgcac cggggtcggt aacaattgag cctgatgcct ctaaagctgc
     4981 agtgcgtatc aactggacgc cagtcaatgc ccagatacgt tatcgcctgg cagtatcgac
     5041 cgatgagtat ttcagcgaag aatacgtcgc taaacaggtc agtggaaaag gcagtgcggt
     5101 tgttaacctg ccgccgggta cttattacta ccgtgttcgg gcagctggca aaagcggtgg
     5161 ccgtgcactg gctgtcagcc aggccagaaa agtagagatc cgccgcaaga aactgccacc
     5221 cccgccgcgc gtgaaatcag tacaggcaga atagtaaacg gggccttcag gcccctgcag
     5281 cctgtgccgt tttttcttga ccacacccgg agcaaaaggc ctttaagcac atgaaagcac
     5341 tatggatgat cttactcgcg gggctgatta cggcaggctg cggccgtggc tatgaagagc
     5401 tgctgcagca gggtacggag cagagaagag cgcagaaatg ggcgcaggca aagcacacgc
     5461 tctttgctgc agtagagaag aagcagaccc ccgaggtcta taaagaactc ggaaatgtct
     5521 ttttactcgg cgaacagaac atcgctgagg cagaaggtta ctaccgcaag gcacttgcca
     5581 tagacgcaaa ctatatcaat gcacagttca acatggcggt tatatcgctg aaaaagtatg
     5641 agctgacgct cgacgacaag ggcaagggcg acgatgcgtt acttaaagaa gctgagggct
     5701 ggtttaacaa agtctacagc caaaacccca atttcgccgt cggtatcgaa gaaatggcaa
     5761 agtaccatta ctataaacac gattatgcca aagcgctcga aaccgcacag aaggctgtag
     5821 ctgtcgacaa ccgcaatgcg aatgcctact cggtgatcgg gcagatctac ctgagcggac
     5881 tcaaggatta caaacaggct tttgagaatt ttgagcaggc gcgttcgctc aacaataagg
     5941 acgccgatgt cgtctacttt ctctgggcga ccagcgagaa gctgcgcaag gcgcaggatg
     6001 ccaaacagtt caagacccgc tacgagcaga tgctcaaaca agagggatta tccgcagagc
     6061 aggccaaaga acgcatcagg cggctggaaa accagctgaa gagctgacac aagttcacac
     6121 ccggtttttt ccgataatta gctgtggggt tgctcgaaaa atctctgaaa aaagcgggta
     6181 aagaccgcga gaaaccaccg caaacgggtg aaaagcgctc cgtgggctgg ttacgaacca
     6241 gcctcggaga gcgcaccagc gcgctggccg acaaaataga cctcgaagag caatacgcgc
     6301 gcctcgaaag ccttgccggc gcagtccctg aaccaccaaa gccctcgctg cgtgaccggg
     6361 cccggcgcct gctgggcaat attgtgccag cggcgcagcc gggcaacgcc cagccagcca
     6421 cagaacaggc gcctgccggc gccgggcgcc cgcacatgac cctgcgccag cgggcagccc
     6481 tgctgatgaa gcgcctcagc gggcagggtg gcaaccaggg tggtgccgag ggtagcggag
     6541 ctccccctgc ggccgggtca actggcgcga ccgggcagca tgatacccac ctggcgcccg
     6601 atgatgcggt tgaccacgaa ctgccaccac ccgctttctt tccctggctg gcgctcagcg
     6661 acgaaggcac cctgcattca cacgtcagct tcggcgacga ccatggccat ctacccgaac
     6721 ctgctgaaac cctggtagcc gcccagccgc aggagcttga gcagagcaag acaccgcttg
     6781 ccgaattcgc cgacacgatt ggccgaatta cggaaaacct gaagaacctg catgtccagc
     6841 tggaccaggg taactttatt cgcggcgtcc aggtagagca acaagggcgt ccggttgcag
     6901 aaccgcagac ggcgcagacc ccgatcatca gccaccatgt gccagttgat tcaacccttc
     6961 cggcggcagg agtggagatc cagggccagc cggtaccgca ggctgcagct gaacccaaag
     7021 atgttttctg gccgcgaaac
//
LOCUS       DAOQJF010000162         7024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5951_length_7024_cov_6.047640, whole genome shotgun sequence.
ACCESSION   DAOQJF010000162 DAOQJF010000000
VERSION     DAOQJF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5951_length_7024_cov_6.047640"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1220
                     /locus_tag="PLF85_09560"
     CDS             117..1220
                     /locus_tag="PLF85_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE19955.1"
                     /translation="MAEFTGLIGTVGTATAFYTGLLLVLGRHRNTVRLLGGAFFFLFA
                     AILLLFARFLLDRYQSMPHLAFVHLPIVVLAGPAIYFFLRFVAEPDFRLKAPYLLHLI
                     PAVAVLFYMSDTYALPAEVKRRMLSHTEPLPEYVIPSLRVVFMLAYTSPLVYFLISVR
                     YLRPYFARLQSREFAFLRALRFFLLTCALATLLMLATLVGGDWMPVKVLALSAYSLAV
                     VLAFVALARNQHFPELLARDARRYDRLKGRSESKVEQLERLLLDNRLYAEENLRVADV
                     AQRLDLRADQLSQLINDHFGSNFNRYINRLRIDDAKRRLQETPELGVLRIAYECGFAT
                     KSAFNAAFKLETGVSPTEFSRKLADSGKKRPES"
     gene            1262..3148
                     /locus_tag="PLF85_09565"
     CDS             1262..3148
                     /locus_tag="PLF85_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE19956.1"
                     /translation="MEKNKLRNVAQLYREAAVSYASEKSFLTRQASGAFDGPTYSELF
                     EKGLDLAAALVAVCGLQAREHVAILADNRLEWIIADYAVILAGAADVPRGTDATEFDM
                     EYIVNHAGCRVVFIENQATYKKFVSVADKLTGVGHVIFIDKVDAGNTPWQCHNMQDLQ
                     QQGKALGKKEVERRISGVKPEDLYTLIYTSGTTGAPKGVMLTHGNVISQIRNLPLGFT
                     RQDVMLSILPVWHIFERAFEVISIASGAKTVYTNVKHLKSDLQAVRPTFMASAPRLWE
                     SIYNGIMARVAEGSPLRRGLFRLAVALNHTYRNALREIRGQNLCMNPQNFLERLASLA
                     SAILLAIITFVPALLLDSIVLTKIRAATGGRLRATISGGGALPLHIDEFFNDIGISVL
                     EGYGMTETSPIISVRLPHNAVIGTVGPLYAETEIRLIDLATGAQIFPGENYFGRKGEL
                     HVRGPQVMAGYYKNAEATAKVIKDGWLNTGDLAVMTANGCLKIVGRSKETVVLMNGEN
                     VEPVPIESKLLESPYIEQCMVVGQDQKFLGVLIVPRAEALKQFGNDLATLAHNAEVKK
                     LLKAETHRLISGDNGFKAFERIGGIAVLNRVWEKDRELTAKLSLRRHVITEMYAAQIA
                     GIFD"
     gene            3298..6744
                     /locus_tag="PLF85_09570"
     CDS             3298..6744
                     /locus_tag="PLF85_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE19957.1"
                     /translation="MRLFLILAFMFPMSAFAAKKMLIMDFRNLDQDPNFQYLEGSITD
                     AVKKLLREKFEFFEPEANDVEKRAQDSYFLFPEDYHNKNVALQLGLMTDQDIVISGGF
                     QKRYSARGEAFILVDVMIFDIENKKLVRQFKNEIKVNAAIFESIDKLAQKVAQEAQEI
                     LPHKGQFEFDRYAPITQTQVTLLGGYNLNSFSSPMHKETVLTSGSSMSAADYGGMWIA
                     AELRRDRFLDRNRLVGFFRADFQMINSQFAVSGTADRSSATGMGGAANFSVVLGTQPS
                     AAVQIPLQSSDTTRGSITVSSLTFTPTDWNIPQYVTATGIDDGAVSDGSHNYSIVTGR
                     AISEDKNYDGLDPVDVLITNADNDLAGVTISPTAGLTTKEAAGAGHTATFTVVLNVAP
                     TGSITINLTSSNTAEGTVSPATLYFSTGNWNSPQTVTITGVADNKVDGNQNYTIITAP
                     IAATGTNYDNIDPANVLVVSQDTDTAGFTIIPVAGLTTSESGASATFTVQLTSQPTAN
                     VQVTLTSDNTSEGQIGRTSGSCDNSSGLSTASCALTFTSANWNTAQTVRITGIDDAST
                     DGNIVYHVVTGPATSSDSNFNGVDPADITLTNSDNDTPGITVSPTAGLTTTEAGGVAT
                     FTVKLNTAPTANVTLTLSSSNEAEGRIRRGTNCDNAGTAGTGSCTLVFTPASWNSAQT
                     VGVVGQNDFVADGSISYSVFFSTPSSSSDTNYNGLTPASVALSNSDNDMAGVTVSAIS
                     GNTAESGTTATFTVVLTSQPLSPVDIYYSSSNTGEGQIKQSLGLCATAGTAAATACKL
                     SFTSANWSTPQTVTVQGINDFVQDGNITYNLTSAAIAGSGADYNGIIPASVTVTNIDN
                     DVAGFTVLPSSGLITTEAGGTATFSVRLNSQPTAAVTIGLSSSNTAEGTVSPASLVFD
                     SSNWSTNQTVTVTGVADNRIDSNVLYSIITAAASSADPIYNNQNPADVSVTNNNSDVG
                     KYIFVTSATYNGNFSAYPASGGFAGTTGADGLCNADAAKPNVSTYKAVIGSYDGGLPD
                     VERDGSGIALDWALYTTTRYYRAADSLLVGTTDANGLFTFPLTNSFHNAAASYWTGLT
                     SLYAYSATSSCRPTGTTTISWNASSTRTGVIGDGLSTISTAIANGSLACSNTLRLLCA
                     EQ"
     gene            6821..>7024
                     /locus_tag="PLF85_09575"
     CDS             6821..>7024
                     /locus_tag="PLF85_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19958.1"
                     /translation="MLTRIVLLLAVLPLLIACGKSRSQVRISVPKTEARITFPQGFLW
                     GTATAAEQLEPTEDSDWAELIRKS"
BASE COUNT         1689 a         1958 c         1828 g         1549 t
ORIGIN      
        1 tctgcatcac catggtggca gacggcgtga agtcgatgga aaaagaagag tcggtgaaga
       61 cacttgacgt cgcggagatt ctggcgcaga ccgtgaagta gattgctccg caatccatgg
      121 ccgaattcac cggcctgatc ggtacggtcg gaacagcaac ggccttttac acagggctgt
      181 tgctggttct gggcagacac cgcaacaccg tgcgcctctt aggaggcgct ttctttttcc
      241 tgttcgcggc gatactacta cttttcgccc ggttcttact cgatcgctac cagtcgatgc
      301 ctcacctggc attcgtacac ctgccaatcg ttgtgctggc agggccagct atctattttt
      361 tcctgcgatt cgtcgccgaa cccgattttc gtcttaaggc tccctacctg ctgcatctta
      421 ttcctgcggt cgcggtactg ttctatatgt ccgacaccta tgcactgccg gcagaagtga
      481 agcgccggat gctctcgcat accgaacctc tgcctgaata cgtcatacca tcgctgcgcg
      541 tggtattcat gctcgcttac acttcgccgc tcgtctactt cctgatttcg gtgcgctacc
      601 tgcgcccgta ctttgcgcgg cttcaaagcc gcgaatttgc atttctgcgg gcactgagat
      661 tctttctgct gacctgcgca ctggcaacgc tgctgatgct cgcaaccctc gtcgggggcg
      721 actggatgcc ggtgaaggtc cttgcgctgt cagcctattc gctcgcagtc gtgcttgcct
      781 tcgtggcgct ggcgcgcaac cagcattttc ctgaattgct cgcgcgtgac gcccggcgtt
      841 atgaccgcct aaagggccgc agcgaaagta aagtggagca gcttgaacgg ctgttgctgg
      901 ataatcgcct gtatgcagaa gaaaacctgc gcgttgcaga cgtcgcgcag cgattggacc
      961 tgcgcgccga ccagctctcg cagctgatca acgaccattt cggatcaaat tttaaccgct
     1021 acattaaccg cctgcgcatc gacgatgcca aacgccggtt acaggaaacc cctgaactgg
     1081 gtgtgttgcg catcgcgtat gaatgcggtt ttgccaccaa gagcgcgttt aacgccgcgt
     1141 ttaagctcga aacgggtgtg tccccgacag aattttccag aaagctcgcg gactcaggaa
     1201 aaaagcgtcc agaatcctga ttcagggcga cggggagcaa aatccgtgct aggctcaggg
     1261 catggaaaag aataaactcc gcaatgttgc gcagctttac cgcgaggcag cagtatctta
     1321 cgcatctgaa aaatcattcc tcacccgcca ggcgtctggc gctttcgacg gcccgactta
     1381 cagcgagctc tttgaaaaag gcctcgacct cgccgccgcg ctggtcgcag tctgcgggct
     1441 tcaggcgcgc gagcatgtgg cgattctcgc cgataaccgt ctcgagtgga ttatcgctga
     1501 ctacgcggtc atactcgccg gcgcagccga tgtaccgaga ggcaccgacg cgacagagtt
     1561 cgacatggaa tatatcgtca accatgcggg ctgtcgcgtt gttttcatcg agaaccaggc
     1621 tacttataag aaatttgttt cggtcgcaga caagctgacc ggcgttgggc acgtcatctt
     1681 catcgacaag gtagatgccg gcaacacccc ctggcagtgc cataacatgc aagacctgca
     1741 gcaacagggc aaggcgctcg ggaaaaaaga agtagagcgc cgtatttcgg gcgtgaaacc
     1801 cgaagacttg tacacgctga tctatacctc gggcacgact ggcgccccca agggagtcat
     1861 gctgacgcac ggcaatgtca tctcgcagat tcgtaacctg ccactgggtt ttacccggca
     1921 ggacgtaatg ctctcgattc tgccggtctg gcatatcttt gagcgcgcgt ttgaagtgat
     1981 atcgatcgcc tcgggcgcga aaacggtcta tacaaacgtc aaacacctga aatctgacct
     2041 gcaggcggtt cggccgactt tcatggcctc tgccccacgc ctgtgggaga gtatctacaa
     2101 cggtatcatg gcgcgcgtgg ctgaaggcag tccactgcgg cgcgggctct ttcgcctggc
     2161 agttgccttg aaccatacct accgcaacgc cctgcgggag atcaggggac aaaacctctg
     2221 catgaatcct cagaatttcc tggagcgcct ggcttcgctg gcatcggcta ttcttttggc
     2281 aataataacg ttcgtaccgg ctctgctgct cgattcgatc gtactaacca aaatccgcgc
     2341 agcgaccggt ggcaggctgc gcgcgacgat ctctggcggc ggcgccttgc cactgcacat
     2401 cgatgagttc ttcaacgaca tcggtatatc cgtactcgag gggtatggta tgaccgaaac
     2461 ctcgccgatc atctcagtac gcctgccgca caatgcggtt atcggtacgg tgggcccgct
     2521 ctacgccgaa accgagatca ggctgattga cctggctacc ggtgcgcaga tatttcccgg
     2581 tgagaattat tttggcagaa aaggcgaact acacgtgcgc gggccgcagg taatggccgg
     2641 ttactacaag aatgccgagg ctacagcgaa ggtcatcaaa gacggctggc tgaataccgg
     2701 tgaccttgcg gtgatgactg ctaacggctg cctgaaaatt gtcggccgca gtaaagagac
     2761 cgtagtactc atgaacggcg agaatgtgga gcccgtgccc attgaatcaa aactcctcga
     2821 atccccgtat attgaacagt gtatggtcgt gggacaggac cagaagttcc tgggagtcct
     2881 gatcgtaccg cgcgcagagg ccctgaaaca attcggcaac gacctggcga cactcgcgca
     2941 taacgcagaa gtcaagaaac tcctcaaggc cgaaacgcat cgactcatta gcggagataa
     3001 tggtttcaag gcgtttgagc gcatcggcgg cattgcggtg cttaaccgcg tctgggaaaa
     3061 agatcgcgag ctcacggcaa aactatcgct gcggcggcac gtcattacag aaatgtacgc
     3121 tgcgcaaatt gccggtatat ttgactaaat acgcccgcat agtcggcaga tcatcttatg
     3181 ctatcatatt gattacggga attctgccgg cacaaattta ctgcacaaga tgaagcgacg
     3241 taaagttgac catactgcta cttatcgcgc ggtaaatgcg caggagccga aaaattgatg
     3301 agactctttc ttatcctggc attcatgttt cccatgtcag cttttgctgc caagaaaatg
     3361 ctgatcatgg atttccgcaa tctggaccag gatcccaatt ttcaatacct cgaaggatct
     3421 attactgacg cggtaaaaaa gctattgcga gaaaaatttg aattcttcga gcccgaggca
     3481 aacgatgtag aaaaaagggc gcaggatagt tactttcttt tccctgaaga ttaccataat
     3541 aagaatgtgg ccttacagtt agggctcatg actgaccagg atatagtgat cagcggtggc
     3601 ttccagaagc gttactcagc gcgcggagaa gccttcatac tcgtggacgt gatgatcttc
     3661 gatatcgaaa acaagaaact cgtcaggcaa ttcaagaacg aaatcaaggt caatgcagca
     3721 atatttgaaa gcatcgataa gctcgcgcaa aaggtcgcac aggaagcaca agaaatactt
     3781 ccacataaag ggcagtttga atttgatcgc tatgccccga ttacacaaac acaggtcacg
     3841 ctgctcggtg gttacaacct gaactcattt tcttctccca tgcacaagga gacggtcctc
     3901 acgagtggtt cttcgatgtc tgccgctgat tacggtggaa tgtggattgc cgcagaattg
     3961 cgccgtgacc gtttccttga tcggaatcgc ctcgttggtt ttttccgcgc agattttcag
     4021 atgattaaca gccagtttgc agtatcgggg acagctgatc gcagctcggc aaccggtatg
     4081 ggcggggctg ccaatttttc cgtagtgctc ggcacacaac cttcagctgc cgtgcagata
     4141 ccactgcaga gctcagacac aacccgcggt tcgattactg tcagctcact cacgtttaca
     4201 ccgaccgact ggaatattcc gcaatacgtg acggcaactg gcatcgacga tggtgcagtc
     4261 tcagatggct cgcacaatta ctccatcgtc acaggaagag caattagtga agacaagaac
     4321 tatgatggtc tggacccggt agatgttttg atcacaaacg cagataatga tttggcagga
     4381 gtaacgattt ctccgactgc ggggttaacg accaaggaag ccgcgggcgc aggccacacg
     4441 gccaccttta ccgtggtcct gaatgtggcg ccaaccggat cgatcacaat aaacctgaca
     4501 tcgtccaata ccgccgaagg gaccgtttcg ccggcaacgc tgtatttcag cacaggtaac
     4561 tggaacagcc cgcaaactgt caccattacc ggtgtcgcag ataacaaggt cgacggcaac
     4621 cagaactaca caattatcac tgcgccgatc gcggcgacgg gaacaaacta cgacaatatc
     4681 gacccggcaa acgtccttgt cgtaagccag gacacagata ctgccggctt tacaatcatt
     4741 ccagttgcag ggctaaccac ttcagaatcc ggcgcctcag ccacatttac cgtgcaactc
     4801 accagccagc cgacggcaaa tgtccaggtt acgctcacat cagacaacac gagcgaaggc
     4861 caaataggcc gcacgtctgg ttcgtgcgac aacagctcgg gcctgtcgac agccagctgc
     4921 gcgctcactt tcacatcggc aaactggaat accgcacaga ccgttaggat aaccggcatc
     4981 gacgatgcaa gtactgatgg caatattgtt taccacgtcg tgacgggccc cgccacgagt
     5041 tccgattcga acttcaatgg tgtcgacccg gcggatatca cgctgacgaa cagcgataac
     5101 gatacgccgg ggattacggt ttcccccact gcgggactca ctaccaccga agcgggcggc
     5161 gtcgcgacgt tcacggtaaa actgaatacc gcgccaacgg caaatgtcac gttaacgctt
     5221 tcgagcagca atgaagccga aggccgcatt cgccggggta caaattgcga caacgcgggt
     5281 acggcaggca ccggctcgtg taccctggta ttcacgccgg ctagctggaa tagtgcacag
     5341 acagtcgggg ttgttggcca aaatgatttc gtggccgacg gcagtatcag ctacagtgtg
     5401 ttcttctcaa ccccgtcatc cagttctgat accaactaca atggattgac ccccgcatcg
     5461 gttgcactca gcaattcgga caatgatatg gcgggagtca ccgtttcggc aatcagcggg
     5521 aacactgctg agtccggaac cactgccacg tttaccgtgg tcttgacttc gcaaccgcta
     5581 agcccggttg acatctatta cagtagctcg aataccggcg aaggccaaat caagcagtca
     5641 ctgggattat gcgcgaccgc aggtaccgca gcagcgactg cctgcaagct gagcttcaca
     5701 agtgccaact ggagtactcc acaaactgtt acggttcagg gtattaacga cttcgtacag
     5761 gatggtaata tcacctataa cctcacctct gctgcgattg ccggctctgg tgctgactac
     5821 aatggaatta ttccggcttc ggtgactgtg acgaatattg acaacgatgt cgccggcttt
     5881 accgtgttgc caagttcagg tctgatcacc accgaagcag gtggcacggc gacgttttcc
     5941 gtcaggctaa attcgcaacc cacggcagca gtgaccattg gcttatcctc gtcgaacaca
     6001 gccgaaggaa ccgtctcgcc tgccagcctg gtcttcgatt catcgaactg gagcaccaac
     6061 cagacagtga ccgtcacagg cgtcgccgac aaccggatcg acagcaatgt tttatattcc
     6121 ataataacag cagcagcaag cagtgcagac ccaatttaca acaaccaaaa tccggccgat
     6181 gtaagcgtta ccaacaacaa ctcagacgtc ggtaagtata tctttgtgac ttcggcgact
     6241 tacaatggca atttttctgc ctatcctgcg tcgggtggat ttgctggtac cacgggtgca
     6301 gacggtctgt gcaatgccga cgctgcaaaa ccaaatgtct ccacctataa ggccgttatt
     6361 ggcagttacg atggcggcct accggatgta gagcgggacg gatccgggat tgcgctggat
     6421 tgggccctct ataccactac gcgctattac cgcgcggcag actctctgct ggtggggacg
     6481 acggacgcaa acggcctgtt cacgtttcca ttgactaata gttttcacaa cgctgctgcg
     6541 agctactgga ctggtttaac aagtctatac gcctactctg cgacttcttc ctgcaggcca
     6601 accggtacaa ctacgatttc atggaatgcc tcttcgaccc gaactggtgt cataggcgac
     6661 gggttatcga ccatcagtac cgctatagca aacggcagtc tggcatgcag caatactctg
     6721 cgtctgctct gcgcagaaca atagccgcta actaaccgct tagggcacat cttccgcggg
     6781 taatatttga caaatctgct agccaataaa cggttgcaga atgcttacac gaatagtact
     6841 cctattggca gttctgccgc tgctcatcgc atgtggcaaa tcgcgctcgc aggtgcgcat
     6901 ctctgtgccc aaaacagaag ctcgcataac ctttccgcag ggttttctct ggggtaccgc
     6961 taccgccgct gaacaacttg aacccactga agacagtgac tgggcagaac tgatacgcaa
     7021 aagt
//
LOCUS       DAOQJF010000163         6999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5984_length_6999_cov_4.883641, whole genome shotgun sequence.
ACCESSION   DAOQJF010000163 DAOQJF010000000
VERSION     DAOQJF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5984_length_6999_cov_4.883641"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLF85_09580"
     CDS             complement(<1..979)
                     /locus_tag="PLF85_09580"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNE19959.1"
                     /translation="MNSSYLQKIYQLTKPLLFKTDAERAHAFASASMRIVTPFIRSSG
                     IKYRTPRVIFDRKVYSPIGIAAGFDKAAAAPLYLHKLGFGFLESGSLTPKAQSGNPKP
                     RLARLPDQEVLINKMGFNNPGFIAGWKRLRSRLAVLPHEYLVALSLGKGKETPVEAAQ
                     DDYTWCLEHLAGDVMAEYLFYVAINVSSPNTPNLRALQTGKAIRSLVESCVKVSPRPV
                     VVKFAPDFVSDKAYRDSLAAALEGGAQGIIISNTTTDHSLATLPASLEGFGGGLSGRP
                     LVEKARHYLSLTLKQTKGKVPVISSGGVFSPDEAKKRIDLGADLVQVYTG"
     gene            complement(1042..>2280)
                     /locus_tag="PLF85_09585"
     CDS             complement(1042..>2280)
                     /locus_tag="PLF85_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE19960.1"
                     /translation="GGREFKSSDQFNRATMAKRQPGSAVKPFVYGAALEARAIHYAMG
                     FVDSPIVNIQPNGEQWAPANFDSSFNGYVLAYRALSASLNLVSVQIYDLVGPDAIINY
                     LSRLTKAPESRFQPNPALSLGASELTPAELLLGYAIIGNKGQDIIPHAIMYIADREGN
                     VIAHPENDVIQALNYKRRTGQIQVIEEGPAFILRKMMENVVNAGTPTHSIRIRAGYKG
                     PGAGKTGTSNSFNDVWFGGYTTDMAAVIWAGMDNGNMTLGRGVSGGDVIAPVWGEFMR
                     DIYNARGKLPEPFDQTLPKGVRQGGVCRYTGKWPNPECDKPENMTGTLIPDVITVNGK
                     RRSVGGETCNCHHAESKSFLDLLQADHSLSNEEVGREGNKAYSLTEGDKLLKQLGGDL
                     PVPGAAPNGGTAPANGNTAP"
     assembly_gap    2281..2380
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2381..3465)
                     /locus_tag="PLF85_09590"
     CDS             complement(<2381..3465)
                     /locus_tag="PLF85_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNE19961.1"
                     /translation="MPLTGSELNPPLKDLPWWQKLEKIVLYGFFLSAFFGGLFLGFLH
                     FRIHSKTGDLDRLASFRPTIPTRLYDQKGRLIAELFRHQRQLVPLDEIPRPVIAAFLA
                     IEDSNFYEHFGIDFKGILRATFKNIRAMRVVAGGSTITQQLVKGLYTESERTLYRKIY
                     EAVLALQVEKAFTKDEILEMYFNQTYFGHGAYGISAAAQFYFDKPVSKLNFMEASILA
                     ALPKSPHNYSPFRAPHEAYKKNKVSLNRLVEVGFITKEEADTLYKTFWPVYWKKIVTT
                     PPTKTIFGEKVNNAPYFTEHVRQELIALYGEEVVYSKGLQVYTTLDLDHQKAAERAYL
                     PQLEKMDPIARRYNQFMTGGIDGQLLGT"
     gene            3548..4486
                     /locus_tag="PLF85_09595"
     CDS             3548..4486
                     /locus_tag="PLF85_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNE19962.1"
                     /translation="MRYSLLSLLLLVTACASLPDKPRLQTIELQGHRGARGLAPENTW
                     PAFEIALQKGMNVLELDTVLTAEGDLVIHHDTDTNPKICRNDDGSAIESKPVRQLTVA
                     QLRQLDCGSLRHGDFSEQVPRPGTRLMTLSEFFRAVKEYENKNKTASPVMFNVEAKFP
                     KGAAPDAAELRLFAATLLKQIRDAGVEKRTTLQSFRIGILPVIKELNPAMRTSALFEP
                     SKWQGLRMYLGLGSGIRRDIIDAAVAARADIISPYRLYANTDFVRDAHARGLAVIPWT
                     VNDPAEMEALLARGVDGIISDYPDRLAAVWNKRQKQ"
     gene            4483..5259
                     /locus_tag="PLF85_09600"
     CDS             4483..5259
                     /locus_tag="PLF85_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19963.1"
                     /translation="MKLAGALITCVLCLPLSGAQKLRDAKFPLTFTPEKSLQVTVQLA
                     DGVAVAQLPAGKQQKLSAYDSTEVGVEAAPLLVGDFNFDGYDDIAVLEGNGYGGVNLF
                     YRIWLWQSKQQNFREFTTSISNPVLNSGSRMLISGHRSGPRWYQAVFAANAGTLLPYA
                     EAQMLDAVAFWGVVFSDGTRTVADAKWFDGDLRKAPQATAELVAGKCGGGQKQNAKAP
                     TNKKLRAVLMDFRNEGAEVLARAGEGAQPRWISAECLASE"
     gene            complement(5286..6119)
                     /locus_tag="PLF85_09605"
     CDS             complement(5286..6119)
                     /locus_tag="PLF85_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19964.1"
                     /translation="MSRLTAMVKIATKFAPAALFMVLATGAQAADDFDLTSEQLEQGA
                     AVQEKPVYGNYLENMRAAEKPRFVTLIDAFQYARGAGLNTRGVLFSYHNRAARDVRFV
                     ADIPGFAPQPMRRNKYGVWIYYYMPPEVVHAAPTKKIRYKFQVDGLYTVDDTHEQHEK
                     DSAGQVASVFHLLHDHFAASTGVFVLDTPVNHGREVLFRIPAGDAESASIAGDFNKWN
                     PQRDYLNRSEDGYFELRKIIPPGEYTFLYRIDGKLSQKVATTKSRVHPVYGRVNYLVV
                     K"
     gene            6186..>6999
                     /locus_tag="PLF85_09610"
     CDS             6186..>6999
                     /locus_tag="PLF85_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19965.1"
                     /translation="MAEEQEKPEVTYTPEELSTIRELTGFFNKAPGQVALDEAGEETA
                     TGEEPFTDEVGLSDGAGEGATDAAAPKRPIADLSKFDDVLNMDLDNFDSAPTGEVQDV
                     PLSDDTAGTDVGMDFGAADIAPAADTTAADEPSDFNFDLPAEPAVETPAMAEFDLGDG
                     AGMDLAPATAEAPTDLPADDFTLPGADTDFGAAPAGDDFSFDTPAAEAPADFDLGTPA
                     ADFGADAAPADTPIDLDFGAADSAPAADFDFGETPAAGADDLGAGFDFPAADA"
BASE COUNT         1412 a         1914 c         2049 g         1524 t
ORIGIN      
        1 agcccgtata gacctgaacg aggtcagctc cgaggtcgat gcgctttttc gcctcgtcgg
       61 gcgaaaaaac acccccgctc gaaatcaccg gcactttacc cttagtctgt ttgagtgtca
      121 ggctaaggta gtgccgcgct ttttcaacca gtggcctgcc cgaaagtccg ccgccaaatc
      181 cttcaagtga tgcgggtaac gtggcgagag aatggtccgt tgtagtgttc gaaataatga
      241 taccctgcgc tccgccttca agcgcagctg cgagcgagtc gcggtaggct ttatcagata
      301 cgaagtcggg ggcgaacttg acgacaacag gccgcggcga aactttcacg cagctttcga
      361 ccagtgaacg aatcgccttg cctgtctgca gcgcgcgcag gttcggagtg ttgggtgagc
      421 tgacgttgat cgccacataa aaaaggtatt ctgccatcac atcgcccgcg aggtgttcga
      481 ggcaccatgt gtagtcgtcc tgtgccgctt ctacgggagt ctctttacct ttgccgaggc
      541 tcagcgcaac aaggtattca tgcggcaaca ccgctaacct gctgcgcagt cgtttccagc
      601 cggcgatgaa gcccgggttg ttgaacccca tcttgttaat caatacctct tgatcgggca
      661 ggcgggcgag gcgcggtttg ggatttccgc tctgcgcctt cggtgtcagg ctgccgctct
      721 cgagaaagcc gaagccgagt ttatgcaggt agagcggtgc agctgcggct ttgtcgaagc
      781 ccgcggcgat gccgatgggt gaatatactt ttcggtcaaa gatgacacgc ggtgtcctgt
      841 acttgatgcc gcttgatcgg ataaacggag tgactatgcg catgcttgcc gaggcgaaag
      901 catgcgcgcg ttctgcatcg gttttgaaaa gcagcggctt cgtcagctga tagattttct
      961 gcagatatga actattcata ttgcccggtt ttaactattg tcatagtgag cctgtcgaac
     1021 tatgactctg cgcgacccgc ctcacggcgc ggtatttcca tttgccggtg ctgtaccgcc
     1081 attcggagct gcacctggca ccggtaaatc accgcccagc tgcttgagaa gcttgtcgcc
     1141 ctcggtgagt gagtaggcct tatttccttc acggccaact tcttcattgc tcaggctgtg
     1201 gtctgcctgc agcagatcga gaaagctttt cgattcggcg tggtggcagt tgcaggtctc
     1261 gccgcccacg cttcgacgct tgccgttaac ggtaatcaca tcgggtatga gggtgcccgt
     1321 catgttctct ggcttatcgc attcggggtt cggccatttg ccggtataac ggcagacgcc
     1381 gccctgccgc acacctttcg gcagtgtctg gtcaaacggt tcaggcagtt taccgcgcgc
     1441 gttgtagata tctcgcatga actcgcccca aaccggggca atcacatcac cgcccgatac
     1501 gccgcggcct aatgtcatgt taccgttatc cataccagcc catataactg ctgccatgtc
     1561 ggtggtgtag ccgccgaacc aaacatcgtt aaacgaatta ctcgtgccgg tcttgccggc
     1621 gcccgggcct ttatagccag cgcgaatgcg aatcgaatgc gtgggagttc ctgcgttgac
     1681 gacgttttcc atcatcttgc gcaagatgaa cgccgggcct tcttcgatga cctggatctg
     1741 cccggtgcgg cgcttgtagt tcaacgcctg tatcacgtca ttttccgggt gggcgatgac
     1801 gttgccttcg cggtcggcaa tgtacatgat cgcatgagga atgatatcct gccccttgtt
     1861 gccgatgatc gcgtagccga gcagcagctc agccggtgta agctctgatg cgcccagcga
     1921 gagcgccggg ttgggctgga accttgactc cggggccttg gtgagccggc tcaggtagtt
     1981 gatgattgca tctggcccca cgaggtcata gatctgtacc gagaccaggt tgagtgaagc
     2041 agagagcgcc cggtaagcca gtacgtagcc gttgaagctc gagtcgaagt ttgccggcgc
     2101 ccattgctcc ccgttgggtt gtatgttgac gatcggcgag tcgacaaagc ccatcgcata
     2161 gtgtattgcg cgagcttcga gtgccgcacc gtagacaaac ggtttgacgg ccgaaccggg
     2221 ctggcgtttc gccatcgtgg cgcggttgaa ctggtctgac gatttaaact cgcggccacc
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgccgagca attgcccgtc
     2401 gataccaccg gtcatgaact ggttgtagcg ccgcgcaatc gggtccatct tttccagctg
     2461 cggcaggtaa gcgcgctcag cggctttctg gtggtcaagg tcgagggttg tatagacctg
     2521 caggcccttc gagtaaacaa cttcttcgcc gtagagcgcg atgagttcct gccgcacgtg
     2581 ctcggtgaag tagggggcat tgttgacctt ttcaccgaag atcgtcttcg tcggcggcgt
     2641 agtgacgatc ttcttccagt acaccggcca gaaggtcttg tacagggtat cggcttcttc
     2701 tttggttatg aatcccactt caacgaggcg gttcagcgag actttgttct tcttatacgc
     2761 ttcgtgtggc gcgcgaaaag gcgaatagtt gtgcggcgac ttgggtagcg cggcgagtat
     2821 cgacgcttcc atgaagttga gctttgaaac cggcttgtcg aaatagaatt gagccgccgc
     2881 tgaaataccg tatgcgccgt ggccaaagta cgtctggtta aaatacatct cgagaatttc
     2941 atcttttgtg aaggccttct cgacctgcag cgccagcacg gcttcgtaaa tcttgcggta
     3001 gagcgtgcgc tcgctctcgg tatagagtcc cttgacgagc tgttgcgtga tcgtcgaacc
     3061 accggcaacc acccgcatcg cccgaatgtt cttgaaggtc gcgcgcagta tacccttgaa
     3121 gtcgataccg aagtgttcgt agaagttgga gtcttcgatc gcgagaaagg cggcaatcac
     3181 cggacgggga atttcgtcga gtggaaccag ctggcgctgg tggcgaaaga gttcggcgat
     3241 cagccgtcct ttctggtcgt agaggcgggt tggtatcgtc ggccgaaacg acgctaaccg
     3301 gtcgaggtcg ccggttttcg aatggatacg aaagtgcaga aagcccagga aaagcccgcc
     3361 gaaaaaggca gacaagaaga agccgtagag gacaattttc tcaagcttct gccaccacgg
     3421 caggtctttg agggggggat taagttctga accggtgaga ggcatccggg tggaattttt
     3481 gcccctcagt catagcgaaa agcgggattt aacgagacgc gctgccgcgt gtctcgttaa
     3541 atggctcatg cgttactccc ttctttcttt gttgctgcta gtgaccgcct gcgcctcgct
     3601 gcccgacaaa ccccggctgc agaccatcga gctgcagggc caccgcggcg cacgcgggct
     3661 ggctcccgaa aatacctggc ctgccttcga gattgccctg caaaaaggca tgaatgtgct
     3721 cgagctcgac accgtactga ctgccgaagg cgacctggtt atccaccacg acaccgacac
     3781 gaacccgaag atctgccgta acgacgacgg cagcgccatt gagtctaagc ccgttcggca
     3841 gctgacagtg gcgcagctca ggcagctcga ctgtggttcg ctgcggcacg gcgatttttc
     3901 tgagcaggta ccgcggccgg gcacaaggct catgacgctg tcggagttct ttcgtgctgt
     3961 gaaagaatac gagaacaaaa ataaaaccgc ttcgccagtg atgttcaacg tcgaggcgaa
     4021 gttcccgaag ggtgctgcgc ctgacgctgc cgagctgcgg ctttttgccg cgacgctgct
     4081 gaagcagatc agggacgcag gtgtcgaaaa gcgcacaacg ctgcagtctt tccgcatcgg
     4141 gatactgccc gtgattaaag aactgaaccc ggcgatgcgt acctcggcgc tttttgagcc
     4201 gagcaagtgg cagggactgc gcatgtacct cgggctgggc agcggtattc gccgcgatat
     4261 tatcgacgcg gctgtggctg cacgcgccga catcatttcg ccttacaggc tttacgcaaa
     4321 caccgacttt gtccgcgacg cacatgcgcg cggccttgcg gtgattccct ggacggtgaa
     4381 tgacccggct gaaatggagg cactgctcgc tcgtggtgtc gacggtatca tctctgatta
     4441 tccagatcgt cttgcagccg tgtggaacaa gagacagaaa caatgaagct ggccggtgcc
     4501 ctgattacct gcgtactttg cctgcctctg tccggtgcgc aaaaactgcg cgatgcaaag
     4561 ttcccgctta ccttcacgcc ggaaaaatct ctccaggtga cggttcaact ggcagacggc
     4621 gttgcggtcg cgcagttgcc cgcgggcaag cagcagaaac tttctgcata cgacagcacc
     4681 gaggtcggtg tcgaagctgc gccgctgttg gtcggcgact tcaattttga cggctacgac
     4741 gacatcgcgg tgctcgaagg caacggttat ggcggagtga atcttttcta ccgcatctgg
     4801 ttatggcaga gcaagcagca gaattttcgc gagtttacta ccagcatcag taatcctgtg
     4861 ctcaacagcg gcagccgcat gctgatcagc ggccatcgtt cggggccacg ctggtaccag
     4921 gcggtatttg cagcgaatgc cggtacgctt ttaccctatg ccgaggcgca gatgctcgat
     4981 gcggttgctt tctggggtgt ggtattcagc gacggcacac gcacggtcgc cgatgcaaaa
     5041 tggttcgacg gcgatctgcg caaagcgccg caggccacag cagaactcgt cgccggtaag
     5101 tgcgggggag ggcagaagca gaacgcgaaa gctcccacaa acaagaaact tcgcgctgtg
     5161 ctgatggatt ttcgtaacga gggtgccgag gtgctggcac gagcaggcga gggtgctcaa
     5221 ccccgctgga tcagcgctga gtgcctggcc agcgagtagc aggcttctga aaaggaacca
     5281 gctagttact tcaccacgag ataattcacg cggccataca ccgggtgcac gcggctcttg
     5341 gtagtggcaa ccttctggct gagtttaccg tcgatgcggt agagaaaagt atattcaccc
     5401 ggcgggataa tcttccgcag ttcaaagtag ccgtcttcgc tgcggttgag gtaatcgcgc
     5461 tgcgggttcc acttgttgaa gtcgccggca atactcgctg attcggcatc acccgcagga
     5521 atgcggaaca atacctcgcg gccgtggttg accggcgtat cgagcacaaa gacaccggtg
     5581 ctggcggcaa agtgatcgtg cagcaggtga aagaccgagg caacctggcc tgcagaatct
     5641 ttttcgtgct gctcatgagt gtcgtcgacc gtgtagagac cgtctacctg gaatttgtag
     5701 cgtatcttct ttgttggcgc ggcgtgcact acctcgggtg gcatgtagta gtagatccag
     5761 acgccgtact tgttgcggcg catcggctgc ggggcaaaac ctggaatatc ggcgacaaaa
     5821 cgcacatcgc gcgccgcgcg gttgtggtaa gaaaacagca cgccgcgtgt attcaggccc
     5881 gcaccgcgtg catactggaa ggcatcgatg agtgtcacga aacgcggttt ttctgccgcg
     5941 cgcatgtttt cgaggtagtt gccgtaaacg ggcttctcct gtaccgccgc gccctgctca
     6001 agctgttcag aagtgaggtc aaaatcgtca gcggcctgcg cacctgtggc gagaaccatg
     6061 aagagcgccg cgggggcgaa tttggttgct attttgacca ttgcagtaag acgcgacata
     6121 aatccctcaa attattttcg gaatctccga taaatacttg agggctaaaa cctcaggctt
     6181 aagccatggc ggaagaacag gaaaaaccag aggtcacgta cacccctgag gaactcagta
     6241 ccatcaggga gctcacgggc tttttcaata aggccccagg ccaggttgcg ctcgacgaag
     6301 cgggcgaaga aactgctacc ggtgaagagc ctttcactga cgaagtggga ctgtccgacg
     6361 gcgccggtga aggtgcaacg gatgcggccg ctccgaagcg cccgatagct gacctgtcaa
     6421 aattcgatga tgtccttaat atggacctcg acaacttcga ttcggcacct accggcgaag
     6481 tgcaggatgt tccgctcagc gacgacacgg caggtaccga cgtcggtatg gatttcggcg
     6541 cggcagatat cgcgcctgct gccgacacca ccgcagctga cgaaccctct gactttaatt
     6601 ttgacctgcc tgccgaacct gccgtggaaa cgccggcgat ggcagagttc gatctcggtg
     6661 atggtgcagg catggatctc gcgcctgcga cggcggaagc gcctaccgat ttgccggctg
     6721 atgattttac cttgcctggc gccgatacag attttggtgc cgcgccagcc ggcgatgatt
     6781 tcagttttga taccccggcg gctgaagcgc cggcagactt tgacctcggg acacctgccg
     6841 ccgacttcgg cgcagatgct gccccggccg acacccccat cgaccttgac tttggcgcgg
     6901 cagacagcgc gcccgcggct gattttgatt tcggcgaaac gccggctgcc ggtgcggatg
     6961 acctcggggc tggttttgat ttccctgcag ctgacgctc
//
LOCUS       DAOQJF010000164         6992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_5993_length_6992_cov_5.039210, whole genome shotgun sequence.
ACCESSION   DAOQJF010000164 DAOQJF010000000
VERSION     DAOQJF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5993_length_6992_cov_5.039210"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..688)
                     /locus_tag="PLF85_09615"
     CDS             complement(278..688)
                     /locus_tag="PLF85_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNE19966.1"
                     /translation="MPLYDRFWLDLETEFIVVEYGKGIAAALAVECCGELTGRCRKFR
                     RSLARRKGLIIPPIRLEVSDSLPAFAYVIWVMGVGVAEGDLIRVTGGNILRMLLAQLR
                     HVCTMYAPRVPTRDQIVAIWQRLEMEKEKYVATK"
     gene            complement(724..1383)
                     /locus_tag="PLF85_09620"
     CDS             complement(724..1383)
                     /locus_tag="PLF85_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19967.1"
                     /translation="MTINIKANSRTYSHPGINCLKRTGLTFAASLLLVVALHASHAYA
                     QEAATEQPQGELSDSPAEPAAPEIPMDAPEPEGSNAASEQQNAEDELTQVRANKTLHR
                     YSVGSIQKQRMLAEVRKKAIKQGLSGQRLERHMRGAKMVVDSVGANSMSLELKEGGKL
                     LFNQGERTKVMNYRIKGNIVEVDKGDGSFRRFARFNGNKSTLSLLDPVHGKETVAVLQ
                     K"
     gene            complement(1485..2114)
                     /locus_tag="PLF85_09625"
     CDS             complement(1485..2114)
                     /locus_tag="PLF85_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNE19968.1"
                     /translation="MLLALIFLLAAEPVKKSNPNKVADFAEIDSKIYRAEMLENFEEG
                     DLAEILEPVQPGAETVVSSDFAPPVLESRRYVSVRLTESDTRRVRLQFKKAPEVRDHC
                     RAFTFWLNVEKPYARLTLIVEDRQGVRHFIDSGDLQFRGWRRMVLPVPQKIRQQDYYL
                     NEKSALKLLGIEIVFSRKYAKGKLPVILIDEIAAEVRKKYEVPPELKNP"
     gene            complement(2152..3297)
                     /gene="queA"
                     /locus_tag="PLF85_09630"
     CDS             complement(2152..3297)
                     /gene="queA"
                     /locus_tag="PLF85_09630"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNE19969.1"
                     /translation="MSDSPNLDTDGVATPSTSRKNCWPAEYHDLDFDLPEELVAKHPV
                     EPADHARLMVIDRAQQSITHDFFYNLGNYLATGDAIVYNATQVEERRVHVKPAGAEKV
                     FECVFLKNVPPLPTAPPPPSGGRPGGGTWQVLMRNIRRLKEGTVLHAAKDAAYEFVLS
                     READKIFVKANRSLGPSDFARIGEMPLPPYMKRAAEAKDSETYQNFFSQQIAEKDKVQ
                     GSVASPTAALHFTQELYARLRAQSVGFYPVCLDIGYGTFAPLTEQNFTTASLHAEHYF
                     IPQATADLLQNGSGKRRIGLGTTSLRALLSFHAYGKNEGETRIFIKPGDALAGVEGLI
                     TNFHLPQSSLLLLVAAFAGRELTARAYREAVLQKYRFFSYGDAMLII"
     gene            complement(3275..4708)
                     /locus_tag="PLF85_09635"
     CDS             complement(3275..4708)
                     /locus_tag="PLF85_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNE19970.1"
                     /translation="MYSEFMSYVTGLARVVLVLFRVALQALLQYFAGRAALVLVIVVG
                     AFALLVLAHHVLPGPLASWYAALDKDKAASASLWRSFDAFCSRSFCHHLLPSPIRHYE
                     LHVKEERGFAAFTAALKAEKAKLPALANPPPAVRYVRDFMPSRADFYRARPGARGPEN
                     VSAIYLTGTSARAGRVDKYLEFSRAQGLTGIVFDIKDVYGFVNYRSALPEVKVTQGKT
                     QPPLGELAPLVAYYKEKKFYTIARVALFQDELQATKFPDRQIKLQSGGPLKTKGRTVW
                     VDPNKKAVQDYNLAIIREVLQSGVDEIQLDYVRYPAEGDWKIAEYHGLKDFDEKPRVI
                     GSYLQRVHALTQSYGAYLSLDVFGVVAWQEPLDIKSTGQNMHVLSQTADVISPMLYPS
                     HFGFSFGGIKNPADKPALFLEEGVQKLRDIAPDKTIRPWIQAFKWRVSNYSPQYITEQ
                     IRGSAKAGGHGYLLWNAGNVYERFSQP"
     gene            4821..5606
                     /locus_tag="PLF85_09640"
     CDS             4821..5606
                     /locus_tag="PLF85_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE19971.1"
                     /translation="MSYFEGKIVLLTGASGGLGSEFATQLMDQGAHLLLSDVKLEALD
                     RFKSYSGKGKVIGMVAADLSNKEGTERFLAEVKKITPCPDMIINNAGIAQAGSFGDVP
                     LDRWEKLMNINLISPMRICHYFLPDMKTRGSGHIVNISSVAGIVPLPGGAPYTASKHG
                     IKGFSDAIREEYHKHGIFVSTVHPFFTRTAILQSERFGMNVDVNDYPQFLIETPENVI
                     RETISAIASKTDFILPGPTAKVLDLARRLTPGLMGLFSRGLLN"
     gene            5628..6530
                     /locus_tag="PLF85_09645"
     CDS             5628..6530
                     /locus_tag="PLF85_09645"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HNE19972.1"
                     /translation="MLAFARTYKRTLIFCLGGAFLLPQVFAATLTLTESVRVFGEYGK
                     LQTLAVSPDGKLLAFSSDYRDQKGDIVVYNLESTEKRTVTKTQYLESQPVFSRDGKDI
                     YFFINEVGTGSIKKVNLADESVTQVTDRRFWCEFPALSPDGKHLVYYSKRDNKYNLYE
                     MNLATNKETRLTTEQNFDFGPVYSSDGQSIYFYSNRAGSSFSLFRYERSNQKISPLFG
                     PGGFTFQPTTDVQGLMLFAVSNVSGNNDVYAISLGKNRVDQITTAAGNDLFPVVDQKM
                     NKLYFISQREGDYAIYGRQFKVTP"
     gene            6536..>6992
                     /locus_tag="PLF85_09650"
     CDS             6536..>6992
                     /locus_tag="PLF85_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNE19973.1"
                     /translation="MQKKKTAATLPYTAEKIHPTCFIHERATVFGDVEMGEFSSLWPG
                     VVLRADMNRFILGKYVNIQDNSTVHSDSKRGVTIGDYTLVGHHAMIHGCTIGRGVLIG
                     IGSVILDDAEIGDGAMITAGCMVRGGTKIPPRALVTPDGSTIKIFEKKAK"
BASE COUNT         1576 a         1973 c         1855 g         1588 t
ORIGIN      
        1 catgttgcac ctgtcaagct agcgatgctc gaatatttgt tgccgcaaaa tttgcatgtg
       61 tactgcgcct tttccgaacc ttcatagagc ttatggcgac ctttgcccga tccgtcaggg
      121 tgccgtgagc aagtactacc cgtcaacgac tgcacgcttg agtatgagtt accacaatat
      181 tcacagtaga attttgacat ttgtttctcc ttctctataa gaatgatcac cgatcaatta
      241 gaggggttta gtctatttcc attcatatca aatcaagcta ttttgtagct acgtacttct
      301 ccttctccat ctcaaggcgc tgccagatcg caacaatctg gtcccgcgtc ggtacgcgcg
      361 gggcatacat cgtgcacacg tgccgcagct gtgccagtag catacggaga atatttccgc
      421 cggttacgcg aatcaaatca ccctcagcaa cgcctacgcc catgacccaa atgacatagg
      481 caaatgcagg cagactatcc gaaacttcta accgtattgg cggaataatc agcccctttc
      541 gacgagcgag ggagcgtcga aatttacggc agcgacccgt gagttcaccg caacactcaa
      601 ccgcaagggc ggctgcaata cctttgccgt attcgaccac aatgaattct gtttcaagat
      661 cgagccagaa acggtcgtaa agcggcatgg cagtattaca catcgtgcag tcgcaaggct
      721 ttactacttc tgtaagactg caactgtctc tttgccatgc accggatcga gcaaagacaa
      781 agtcgacttg ttaccattaa accgcgcaaa ccggcgaaag ctgccgtcgc ctttgtcaac
      841 ttcgacgata ttccctttaa tgcggtaatt catgaccttc gtccgctcac cttggttgaa
      901 cagcagcttg ccgccctctt taagttcaag agacatagag ttcgcaccaa cactatcaac
      961 aaccattttg gcacctcgca tatgtctctc cagtcgttgg cccgataatc cctgcttgat
     1021 cgccttcttg cgcacctcgg ccagcatgcg ctgcttctga atcgaaccca cgctgtacct
     1081 gtgtagcgtt ttgttggcac gaacctgcgt caattcatcc tctgcgtttt gttgctcgct
     1141 tgcggcattg gagccctctg gctcgggggc atccatgggg atttcggggg cggcgggctc
     1201 tgctggcgaa tcagacagtt ctccctgggg ctgttcagtg gctgcctctt gtgcgtatgc
     1261 gtgacttgca tggagtgcga ccacaagcaa caggcttgcc gcaaaggtta agcctgttcg
     1321 ctttagacaa ttgatgccag gatgcgaata ggtcctggag ttggctttga tattgatagt
     1381 catagaattc tccttcttat gaataatcta tgactacgcg acgttttcga gggacacgga
     1441 cgcgtccctt ttcctctccc caggggtaaa tttaaaccta ccccctaagg attctttagt
     1501 tctggtggca cttcgtattt cttgcgcacc tcggcggcaa tttcgtcgat gagtatcacg
     1561 ggtaacttac ccttcgcgta cttgcgggag aagacaatct caatccccaa aagtttgagt
     1621 gccgattttt cgttcaggta gtagtcctgc tggcgaatct tctgcggcac gggcagcacc
     1681 atgcggcgcc agcctcgaaa ctgcaggtcg cccgaatcga tgaagtggcg taccccctgg
     1741 cggtcttcga caatcagcgt gaggcgcgcg tacggttttt ccacattgag ccagaaggtg
     1801 aaagcgcggc agtggtcgcg tacctcggga gcttttttga actgtaaccg tacccggcgc
     1861 gtgtcacttt ccgtcagccg cacgctcacg taacgccgcg attcgagtac cgggggtgcg
     1921 aagtctgaag acactacagt ctcggctccg ggttgtacag gttccaggat ttctgccagg
     1981 tcaccctctt caaaattttc gagcatttcg gcgcggtaga tcttgctgtc gatctcggcg
     2041 aagtcagcaa ctttgttggg attgcttttt ttgaccggtt cagccgcgag caagaagatg
     2101 agcgccagga gcattttacg gtcactgagc gcagtcgaag tgtccgtgaa atcaaattat
     2161 cagcatcgca tcgccgtaag aaaaaaaacg gtacttttgc agcaccgcct cacggtaagc
     2221 gcgcgcagtc agctcacggc cggcaaaagc ggcgacgagc aatagcaggc tcgactgcgg
     2281 caggtggaag ttcgtgatca aaccttcgac acctgcgaga gcatcaccgg gtttaatgaa
     2341 gatccgggtt tcgccttcat tctttccata cgcatgaaag gacaagagcg cacgcagcga
     2401 tgtcgtaccc aacccaatcc gccgcttgcc agaaccattc tgcagcaggt cggcagtcgc
     2461 ctgcggtatg aagtagtgct ctgcgtgcag gctggcggtc gtgaagtttt gttcggtcag
     2521 tggcgcaaag gtaccataac cgatgtcgag gcagaccggg taaaagccaa ccgactgggc
     2581 acgcagcctg gcataaagct cctgcgtaaa gtgcagcgcg gcagtcggtg aggcaacgga
     2641 accctgcacc ttgtcttttt cggcgatctg ctggctgaag aaattctggt aagtttcact
     2701 gtctttggcc tcggcagcgc gtttcatgta aggcggcagc ggcatctcgc cgatgcgggc
     2761 gaagtctgac gggccgaggg agcgattcgc ttttacgaag attttatctg cttcgcgact
     2821 gagcacaaat tcatacgcag catctttcgc cgcgtgcagc actgtgccct cttttagccg
     2881 acgaatgttg cgcatgagca cttgccatgt cccccctccc ggccttcccc cgcttggggg
     2941 gggaggtgcc gtaggcagag gggggacatt cttcaagaac acacactcaa acaccttctc
     3001 tgcccctgcc ggtttcacat gaacccgccg ctcttcgacc tgcgtggcat tgtagacaat
     3061 cgcatcgccc gtagccaggt aattgcccag gttataaaaa aaatcgtgcg tgatcgactg
     3121 ctgtgcgcgg tcgatcacca tcagccgcgc gtggtctgct ggttcgaccg ggtgtttggc
     3181 gaccagttct tcaggcaggt caaaatcgag gtcgtggtat tctgcgggcc aacagttttt
     3241 tctggacgtg gaaggcgtgg ctacgccgtc tgtatcaagg ttgggagaat cgctcataca
     3301 cgttgcccgc attccacagc aaatacccgt gcccgccagc tttggcagaa ccgcgaatct
     3361 gctcagtaat gtactgcggt gaataattac tcacgcgcca tttgaatgcc tgtatccagg
     3421 ggcgaatggt cttgtcgggc gcaatgtcgc gtaacttctg aactccctct tcgagaaaaa
     3481 gcgcgggttt gtcggcggga tttttgatac cgccaaacga aaagccaaaa tgtgacgggt
     3541 agagcatcgg tgaaatgacg tctgccgtct gcgagagcac gtgcatattc tggccggtgc
     3601 tcttgatatc gaggggctct tgccaggcaa cgaccccgaa gacgtcgaga gaaaggtacg
     3661 cgccatacga ctgcgtgagc gcgtgcaccc gctgcaggta cgaaccgatt actcgtggtt
     3721 tctcatcaaa gtctttcagg ccatggtact cggcaatctt ccagtcgcct tctgcggggt
     3781 aacgcacata atcgagctgt atctcatcga cacccgactg cagtacctcg cggataatcg
     3841 cgaggttata atcctgcacg gcttttttgt tggggtcgac ccaaaccgtg cgccccttgg
     3901 tcttgagcgg cccacccgat tgcagcttga tctgccggtc gggaaatttc gtcgcctgca
     3961 gttcatcctg gaaaagcgcg acgcgcgcga tcgtgtagaa tttcttttct ttgtagtaag
     4021 cgacgagtgg cgccagctca cccagcggtg gctgggtctt gccctgcgtg accttcactt
     4081 cgggcagcgc ggagcgatag ttgacgaagc cataaacatc cttgatgtca aagacaatac
     4141 ccgtcaggcc ctgtgcgcgg ctgaactcga ggtacttgtc gacccgacct gcgcgggccg
     4201 atgtacccgt gaggtagatg gctgaaacat tctcaggccc gcgcgcaccg ggacgagccc
     4261 ggtaaaagtc ggcacggctg ggcataaagt cgcgcacgta acgcaccgcg ggcggcgggt
     4321 tagccagcgc cggtaacttt gctttttctg ccttgagtgc ggcagtgaag gcggcaaaac
     4381 cgcgttcttc tttcacgtgc agttcgtagt ggcgaatggg cgaaggcagc aggtgatggc
     4441 aaaaactgcg gctgcagaag gcgtcaaatg accgccagag cgaggccgat gccgccttgt
     4501 ctttgtcgag cgccgcatac cacgaagcaa gcgggccagg cagtacatgg tgtgcgagta
     4561 ccagaagcgc aaaagctccc acgactatca ccaggacaag cgcagcgcgg ccggcaaagt
     4621 attgcagtag cgcttgcaac gccacgcgga agagcaccag tacaacccgc gcaaggcccg
     4681 ttacataaga cataaattca gaatacaccc gtctgcgttg caggttgaga cagcgcgtaa
     4741 actatgggcg cgggcggcca taccttccgc gcccattgca caattgttga cactacgttc
     4801 agtttttcct gctgcacacc atgagttatt ttgaaggcaa gatcgttctg ctcaccggtg
     4861 catcgggtgg tctcggcagc gagttcgcca cgcagctgat ggaccagggt gcgcacctgc
     4921 tgctctcgga tgtgaagctc gaggcgcttg atcgtttcaa atcttattcg ggtaaaggga
     4981 aagtgatcgg catggtggct gcagacctca gcaacaaaga aggcaccgaa cgttttctcg
     5041 ctgaagtaaa aaaaataact ccgtgccccg acatgatcat caacaacgcg ggcattgcgc
     5101 aggcaggctc attcggcgac gtgccgctcg accgctggga aaagctgatg aacatcaacc
     5161 tgatatcccc catgcgcatc tgccactact ttctgcccga catgaaaacg cgcggcagcg
     5221 ggcatattgt gaatatttca tcggttgccg gcatcgtgcc acttcccggc ggagctccct
     5281 acaccgcgtc aaaacatggt attaagggct tctctgacgc cattcgtgaa gagtaccata
     5341 agcacggtat ttttgtctct acagtacacc cgttcttcac ccgcacggca attctgcaat
     5401 cagagcgctt cgggatgaac gtcgacgtca acgactaccc gcagtttttg attgagactc
     5461 ctgaaaacgt catccgcgag accatctctg ctatcgccag caagaccgac tttattttgc
     5521 ccgggcccac ggcaaaagtc ctcgacctgg cacgccggct gacacccggg ctgatgggcc
     5581 tctttagtag aggcttgctg aattaggccg gttatgcaag atggcgaatg ctggcattcg
     5641 ccaggacata taagagaaca ctgatctttt gcctcggcgg agcttttctt ttgccgcagg
     5701 tttttgcggc aacgctgact cttaccgagt cggtacgcgt ctttggcgag tacggcaagc
     5761 tgcagacgct cgccgtttca cctgacggta aactgctggc cttcagttct gattaccgcg
     5821 accaaaaggg cgacattgtc gtctacaacc tggaaagtac agaaaagcgc accgttacca
     5881 agacccagta cctcgaatcg cagccagtct tttcccgcga cggcaaagat atctattttt
     5941 tcatcaatga ggtgggcaca ggttccatca agaaggtcaa cctggcagac gagagcgtta
     6001 cccaggtaac ggatcgccgc ttctggtgcg aatttccagc gctctcgccc gatggtaagc
     6061 accttgttta ttactccaag cgtgataata agtacaacct gtacgaaatg aacctggcaa
     6121 ccaacaaaga gacccggctg acaaccgaac agaactttga tttcggcccg gtctattcgt
     6181 cagacgggca gagcatctac ttctactcca accgcgcggg cagttcattt tccctgtttc
     6241 gctacgaacg ctcgaaccag aaaatctcgc cgctctttgg ccccggcgga tttacctttc
     6301 aaccgacgac cgacgtccag ggtctgatgc tttttgccgt ttcgaacgtt tcgggtaaca
     6361 acgacgtcta cgcgatctcg ctcggcaaga accgggtcga ccagatcacc accgctgccg
     6421 gtaacgacct gttcccggtg gtcgaccaga aaatgaacaa gctctatttt atcagccagc
     6481 gcgaaggcga ctacgccatc tacggccggc aattcaaagt aactccgtaa gaactatgca
     6541 aaagaagaag actgcagcca cgctgccata taccgccgaa aaaatacatc ccacctgctt
     6601 cattcacgag cgggccaccg tattcggcga cgtcgaaatg ggggagttct catcgctctg
     6661 gcccggcgtc gtgctgcgcg ccgacatgaa ccgcttcata ctgggcaagt acgtcaacat
     6721 ccaggacaac tccaccgtgc acagtgattc gaaacgcggg gtcacgatcg gcgactacac
     6781 gctggttggg caccacgcca tgattcatgg ctgcactatc ggccggggag tactcatcgg
     6841 catcggcagc gtcattctcg acgacgccga aatcggcgac ggggcgatga tcactgccgg
     6901 ctgcatggta cgcggtggta ccaaaattcc cccgcgcgcg ctggtaaccc ccgacggctc
     6961 aacaatcaaa atcttcgaaa aaaaggcaaa aa
//
LOCUS       DAOQJF010000165         6988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6001_length_6988_cov_4.886773, whole genome shotgun sequence.
ACCESSION   DAOQJF010000165 DAOQJF010000000
VERSION     DAOQJF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6001_length_6988_cov_4.886773"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PLF85_09655"
     CDS             <1..991
                     /locus_tag="PLF85_09655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE19974.1"
                     /translation="MQKILIISKKSLGDIILQTGILQAMKEAHPRMQIFLACDEKNAS
                     AVRMHPDIAEVIPLQLRANGQAAGFFSVLKLIRRHNFDAILTFGRDTQACAWGFFARI
                     PIRVGVRNQPLGFLLTRSLREHETELNSFEFYHKLALLVAPQLRLQFPVLAIDKKESA
                     KLRASLFKQKIPRRFILWHIGSSLEEKRYPVFQIVETLRLFKKKRISLPVLFAAGPGD
                     DAFIAELESAVNRSELKSAKVYFVDGIQFSATVSLLSLAHIVVCNDAAPRHIAAALGK
                     KSLTLMPKHRRHSWQVYGEEQKAYFLFSDAPVESRAVDTIEPAEIAGMIRKLI"
     gene            988..2013
                     /gene="trpD"
                     /locus_tag="PLF85_09660"
     CDS             988..2013
                     /gene="trpD"
                     /locus_tag="PLF85_09660"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNE19975.1"
                     /translation="MTYSAILEKLLAQQNLLTEEITWLIAETMHGRLTPAQVAGWLVA
                     LRNKGETPEEVAACARTMRNMATPIRRTELPPLFIDTCGTGGDRSHLINISTLVGLTL
                     AALGIPVAKHGNRAVSSASGSADVLEACGYPLEENPELITARLASRNFAFFFAPLYHP
                     AMKHAGPVRRELGVRTVFNILGPLANPAGAPVQLMGVYDKALLMPVAETLRLLGVRAA
                     LVVHSDDGLDEISPLAKTSYALLQPSGITGGVYSPPPQERLGSLDSLKATTKESSLAM
                     FRQVIDGQLPAGAYMTALNAAFARKLFEAVQSGNDLKDPAPTADEITEVFSLIQSGAL
                     KGLLTFI"
     gene            2059..3282
                     /locus_tag="PLF85_09665"
     CDS             2059..3282
                     /locus_tag="PLF85_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNE19976.1"
                     /translation="MSEWIHAHKELLVYLSMPITSAFVGWLTNWIAIKMTFYPLNFWG
                     IPPYIGWQGIIPRKAHKMASKSVELMTAKLIKVEEIFDKVKPHEMARELAEPMKKVTS
                     ELVDEVGRTANQTVWDLTPGAVREQIAKQVNKETGPLLETTINEIKRNIYQVFDLKSL
                     VIKNLTGKNVVLLNEMFQRCGKKEFTFIALSGIYFGLPFGVIQMAQWYFYQAPWTVPI
                     VGVIVGYYTNWLALKMIFRPLREKKIMGLVKYQGLFLKRQKEVSKEYADIVANKILSP
                     HQVLENFIYGRAAEAFFSVIQSNVIRALSKLEGMAQPVLSFAVGSERYESLKELVVRR
                     INEAVPKYATTVEGYLGQAMDLEKTLYERLSTLPPEDFEELLRTAFQEDEWILILVGS
                     ALGFGVGLIQMLFIL"
     gene            complement(3353..3658)
                     /locus_tag="PLF85_09670"
     CDS             complement(3353..3658)
                     /locus_tag="PLF85_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19977.1"
                     /translation="MQRIYYSRLGLPDNIEETGTADFTIRWEVSYKEGERDQIDAHSY
                     DEIQELLLHKVEVILKEKNSDLARIVQSSVHHKTKYDPWVSEITGDYKAVLYRLTKS"
     gene            3758..4528
                     /locus_tag="PLF85_09675"
     CDS             3758..4528
                     /locus_tag="PLF85_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19978.1"
                     /translation="MVFRHLAILLASFAMGCEVKNCYQEAKEKHLEKRYGNRPASKDQ
                     LESWEKEIAGYEKVISEKVEAGIKTGKVYRKIGESYALMESYELCIRNLDKAVEFGEA
                     VAEVFYWQGLCYANLSRVHNWQPETARKAEQAFLKALALDGTMNKVKYELAVMYYAAF
                     TRNNQYRVLNDVLTVSQQQFQDKAIELMQEYKSVEPDQSKAYFFLSGLYKERGELADA
                     KRELQQLMAFIEKNNKASFRRNADYLRAEEYLKNFERK"
     gene            complement(4562..>5239)
                     /locus_tag="PLF85_09680"
     CDS             complement(4562..>5239)
                     /locus_tag="PLF85_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE19979.1"
                     /translation="PIRVSEMRIMLSPFQGSRGIMPGGNTLSKQKLEDMGLTVEAMPA
                     DVKGKVQARDKYQIRQIFEDEIKKKKIKVVDEERGIVISLIGDGYFAPGSAKLTEQGR
                     TILTKISDVLARMDSFTRIEGYADDEMTARPAGEFYESAWELAAQRAINALRFLEEKN
                     EVPAEKLSAATYGKTRQLTQSGTPEARALNRRIDVIILSGKSQSRTYKDSALPADRVP
                     QTEASVP"
     assembly_gap    5240..5339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5340..5965)
                     /locus_tag="PLF85_09685"
     CDS             complement(<5340..5965)
                     /locus_tag="PLF85_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE19980.1"
                     /translation="MDKAYMIGFGVAIGVLVLGVEASGVAWGALYDLASIFITFGGAA
                     ATTMMQNPFELTMNIHKIYKLAWLKNEIDINRYVLQIVSLSEIARREGILALEDHVDK
                     LDSKFLTKALQMIIDGTDPEIAKRVMRIEIEALEARHAGNRGWFDTLATMGPAFGMLG
                     TLIGLVGMLSNLGGDTSGLGKAMAAALITTLYGSFLANVFAIPTNNKL"
     gene            complement(6050..>6988)
                     /locus_tag="PLF85_09690"
     CDS             complement(6050..>6988)
                     /locus_tag="PLF85_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNE19981.1"
                     /translation="NQKKISYRAKEATKCPVCGTTHFKEELLSGSGRLIAGKLTQELQ
                     RLYEDNKKWGKINPLVYHVQVCPKCLFAAFPKDFTNLNAEEILALKNTVSHRQKVVET
                     FFGKYDMGENRSIVHGAISYMLNVDCYHLRGNQVAPTPKKAVSAIRAAWILDDLFKEA
                     NYRPYDKLRDFYYVEAARYYKRVLDTMTSGIEPMEEAAYILGPSLDFNWAFDGVIYLN
                     SYLTRKFIDQMATNPKDKYTIMDSSRRYLSKLYGSGKSSKSRPSVIIDMAKELYDEMG
                     KLMEKYRIEYDPVFAEQKAKEAAEAAAIVAQAQAGR"
BASE COUNT         1534 a         1836 c         1898 g         1620 t
ORIGIN      
        1 catgcaaaaa atcctgatca tttccaaaaa atcgctgggc gatatcattc tgcagaccgg
       61 tattctgcag gcgatgaaag aagcacatcc gcggatgcag atttttctgg cctgtgacga
      121 gaaaaatgcc tcggcggtgc gtatgcaccc cgacatcgct gaagttatac cgctgcagct
      181 cagggcaaac ggccaggcag cgggattttt ttcggttctg aagctcatcc gccgccacaa
      241 tttcgacgcc atacttacgt ttggccgcga tacacaggcg tgtgcctggg gattttttgc
      301 gcgtatccca attcgcgttg gtgtgcgtaa ccagccgctg ggttttcttt tgacccgctc
      361 actgcgtgag cacgagacag aattaaattc cttcgaattt taccacaagc tggcattgct
      421 cgtcgcgcca cagctgcgcc tgcagtttcc tgtcctggcc atcgacaaga aagaatcggc
      481 aaaactgcgt gctagcctgt ttaagcagaa gataccgcgt cgttttatcc tctggcatat
      541 cggctcgagc cttgaagaaa aacgttatcc cgtattccag attgtggaaa cacttcgcct
      601 gtttaagaaa aagcgcatct cgctgcctgt tttgtttgcg gcaggcccgg gtgacgacgc
      661 ctttattgcc gagctcgaat cggcggtcaa ccgcagtgaa ctcaagtcgg cgaaagtata
      721 ttttgtcgac ggtatacagt ttagcgcgac agtctcattg ctttcgctcg cgcatattgt
      781 cgtctgtaac gatgccgctc cacgccacat cgctgctgct ctcggtaaaa aaagcctgac
      841 tctcatgcca aaacaccggc gccatagctg gcaggtttac ggcgaggagc aaaaagcata
      901 tttcttattc tccgatgccc cggttgaatc gcgcgcggtc gataccattg aacctgcgga
      961 gattgccggc atgatcagga aactcatatg acctattcgg cgatactcga aaagctgctc
     1021 gcgcagcaga atcttctgac agaagagatt acctggctga ttgcagagac catgcatggc
     1081 cggttgacgc ctgcgcaggt agccggctgg ttggtcgccc tgcggaacaa gggtgaaaca
     1141 cccgaagagg tagcagcctg cgcgcgcact atgcgcaaca tggcgacacc gatccgtcgt
     1201 acagagctgc cgccgctatt catagatacc tgtggcaccg gtggcgaccg ctcgcacctc
     1261 atcaatatct cgactctcgt tgggcttacg ctcgcggcgc tgggaattcc cgtggcgaag
     1321 cacggcaacc gcgcggtatc gtctgcctcg ggttcggccg atgtgctcga agcctgcggt
     1381 taccctctcg aagaaaatcc tgaacttatc accgcacggc tcgcctcgcg caactttgcc
     1441 ttcttcttcg ctccgttata ccatccggcg atgaagcatg ccgggcctgt gcggcgcgaa
     1501 ctgggagtac gcacggtgtt caatattctt ggcccgctcg cgaacccggc tggtgcgccg
     1561 gtacagctga tgggtgtata cgacaaagca ctactgatgc ccgtggccga gacactgcgg
     1621 ctgctaggcg tgcgcgcagc gctcgtcgtg cactcagacg atggtctcga cgaaatatcg
     1681 ccgctggcaa agaccagcta cgctctgctt cagccttctg gtataaccgg tggcgtgtat
     1741 agcccgcccc cgcaggaacg cttgggcagc ctcgattctc tcaaggcgac cacgaaagag
     1801 tcgtcgctgg ctatgttccg tcaggtcata gacgggcagc tgcccgcggg ggcttacatg
     1861 acagcgctca atgcagcatt tgcccgcaag ctttttgagg cagtgcagtc gggcaatgac
     1921 ctgaaagatc cggcacctac ggccgatgaa atcaccgaag tcttcagcct gatccagagc
     1981 ggggcgctca aaggcctgct gaccttcatc tgactctaaa ttgctcgacc ggtaaacttc
     2041 ggacactaac gtgtccgaat gtctgaatgg atccacgcac ataaagagct gctcgtttac
     2101 ctgagtatgc cgataaccag cgccttcgtc ggctggctga ctaactggat tgcgatcaag
     2161 atgacgtttt accccctgaa tttctgggga attccgcctt atattggctg gcagggtatc
     2221 ataccgcgca aggcgcacaa aatggcctcg aaatcggtcg agctgatgac cgcgaagctg
     2281 atcaaggtcg aagagatttt tgacaaggtc aagccgcatg agatggcgcg cgagctggcc
     2341 gaaccgatga agaaggtcac cagcgagctg gtcgacgaag tagggcgcac tgccaaccaa
     2401 actgtgtggg atctcacacc cggtgcagtg cgcgagcaga tcgcgaagca ggtgaacaaa
     2461 gaaacgggac ctctgctcga gacgacgatc aacgaaatca aacgcaatat ctaccaggta
     2521 ttcgatctca agagcctggt gatcaagaac ctgacaggga aaaatgttgt gctgctcaac
     2581 gaaatgttcc agcgctgcgg caagaaggag ttcaccttta ttgcgctcag cggtatctac
     2641 ttcggtctgc cgttcggtgt gatacagatg gcgcagtggt atttctacca ggcgccctgg
     2701 actgtgccga tcgtcggggt gatcgtcggt tattacacca actggctggc gctgaagatg
     2761 atattccgcc cgctgcgcga gaaaaaaatc atgggcctgg tgaaatacca gggtctcttt
     2821 ctcaagcggc agaaagaggt gtcgaaggag tatgcagaca tcgtcgccaa caagattctt
     2881 tcgccacacc aggtgctcga gaacttcatc tacggtcgtg cggctgaggc tttcttcagc
     2941 gtgatccaat cgaacgtgat tcgcgcgctg tcaaaactcg agggtatggc acaacccgtg
     3001 ctgagctttg ccgtgggttc agaacgttat gaatcgctca aagagctggt ggtccgccgt
     3061 atcaacgaag cagtgccgaa gtacgcgacg actgtcgaag gttaccttgg ccaggcgatg
     3121 gacctggaaa agactctcta cgaacgcctg agcactctgc cgcctgaaga ctttgaagaa
     3181 ctgctgcgca ccgctttcca ggaagacgaa tggatcctga ttctcgtagg ttctgcgctc
     3241 ggcttcggcg ttggcctgat ccagatgctg ttcatactat aacagcagtt cggtcaaaca
     3301 ctgcaacggg ggagtgtgcc tcgtatcgcc taagctcccc cgggcaaacc acttacgatt
     3361 ttgtcaggcg gtacagcacc gccttgtaat cgccggtaat ttcagaaacc cacgggtcgt
     3421 atttggtttt gtggtgcaca gaagactgaa caatacgcgc caggtcggag tttttttcct
     3481 ttaagatgac ctcgacttta tgcagcagta gctcctgtat ctcgtcgtag ctgtgcgcat
     3541 cgatctggtc gcgttcgccc tctttataac tgacctccca gcggatcgtg aaatctgcgg
     3601 tgccagtttc ttcgatgtta tccggtaggc caaggcggga gtaatagatg cgctgcatgt
     3661 ttttcctgcc tgagctcggc atcagtctgt caacggcgaa tcggcagggt agatttttat
     3721 ctttacaatc tgcctgtatt atgtcacatt atacccgatg gttttccggc atctggcaat
     3781 tctgctggcc tcgttcgcga tgggctgcga ggtgaagaac tgctaccagg aagctaaaga
     3841 gaagcacctc gaaaaacgtt acggcaaccg accggccagc aaagaccagc ttgaaagctg
     3901 ggagaaagaa atagcaggct acgaaaaggt catcagcgaa aaagttgagg cgggcattaa
     3961 gacggggaaa gtctaccgga agatcggcga atcgtacgct ttgatggaaa gttacgagct
     4021 gtgcatccgc aacctggaca aagctgttga attcggcgag gcagttgccg aagttttcta
     4081 ctggcagggt ctctgctacg caaacctttc gcgcgtgcac aactggcagc ccgaaaccgc
     4141 gcgcaaggca gagcaggctt ttctcaaggc tctcgcgctc gatggcacga tgaacaaggt
     4201 gaagtacgag ctcgcggtga tgtactacgc ggcgttcacg cgcaataacc agtaccgggt
     4261 gctcaacgat gtcctgactg tcagccagca gcagttccag gacaaggcga ttgaattgat
     4321 gcaggaatac aagtctgtcg agcctgacca gtctaaagcc tacttttttc tctctggcct
     4381 ctataaagaa cgcggcgagc tagccgatgc aaagcgtgaa ctgcagcagc tcatggcatt
     4441 tattgaaaag aataacaaag ccagctttcg caggaatgca gattacctgc gggctgagga
     4501 gtatctgaag aactttgagc gcaagtagaa aacgcgcact gcgcgttttc tacttatgct
     4561 tttaaggtac gctggcttcg gtttgcggta cacgatccgc aggcaacgcg gagtctttat
     4621 acgtacgcga ctggcttttg cccgacagaa taatcacgtc tatacggcga ttgagcgcgc
     4681 gcgcttccgg ggttcccgat tgcgtgagct ggcgggtttt gccataagtc gccgctgaca
     4741 gcttctcagc cggtacttca ttcttctctt cgagaaagcg cagcgcattg atggcgcgtt
     4801 gcgctgcgag ctcccaggcg gattcataga attcgcccgc cggccgcgca gtcatttcgt
     4861 cgtctgcgta accttcgatg cgcgtgaaag agtccattcg ggcaagtaca tcggaaatct
     4921 tggtcaggat agtgcgcccc tgttcggtca gttttgccga ccccggggca aaatagccgt
     4981 caccgatgag gctgatgaca atgccgcgct cttcgtcgac aaccttgatc ttctttttct
     5041 tgatctcatc ttcgaagatc tgcctgatct ggtatttgtc gcgcgcctgc accttaccct
     5101 tgacgtctgc aggcatcgct tcgacagtta atcccatatc ttcgagcttc tgcttcgata
     5161 gggtgttacc gcccggcatg ataccccgcg aaccctggaa gggggagagc atgatgcgca
     5221 tttcagagac gcggataggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     5341 tcagcttgtt gttggtagga atcgcgaaaa cgttggcgag aaatgaaccg tagagtgttg
     5401 taataagagc ggcggccatc gctttaccga gaccagaggt atctccaccg aggtttgaaa
     5461 gcatacccac gagaccgatc agggtaccta acatcccgaa agcagggccc attgtcgcga
     5521 gcgtatcgaa ccagccgcgg ttacccgcgt ggcgtgcctc gagggcttcg atttcaatgc
     5581 gcatgacgcg cttggcaatc tcggggtcgg taccgtcgat gatcatctgc agggcttttg
     5641 tcaggaactt gctgtcgagt ttgtcgacat ggtcttcaag cgcgagaata ccttcgcggc
     5701 gggcgatttc agagagtgat acgatctgca gcacgtaacg gttgatatca atttcgttct
     5761 tcaaccatgc cagcttatag atcttgtgaa tattcatggt gagctcaaaa gggttctgca
     5821 tcatcgtggt tgccgcagcc ccaccgaagg tgatgaagat cgaggcaagg tcatagagcg
     5881 cgccccaggc aacgcccgag gcttcgaccc ccagaacgag gacgccgatg gcgaccccaa
     5941 agccgatcat gtatgcttta tccatggtac tattccatta gattttcgga atagaaccgg
     6001 aagggttgta ggcttttttt gttggccggt agatagcccg ctttgcgggc tatctaccgg
     6061 cctgtgcctg tgctactata gccgcggctt ctgcggcttc tttggccttc tgctcggcga
     6121 aaaccggatc atactcgata cggtattttt ccatcagctt gcccatctca tcgtagagtt
     6181 ctttcgccat atcgatgatg accgacgggc ggcttttcga cgatttgccg ctgccgtaca
     6241 gttttgacag gtagcggcgc gagctgtcca tgatggtgta cttatccttg ggattcgttg
     6301 ccatctggtc gatgaacttg cgcgtcaggt acgagttcag gtaaatcacc ccgtcaaatg
     6361 cccagttaaa gtcgagcgag gggcccagga tgtaagccgc ttcttccatg ggctcgatac
     6421 ccgaggtcat ggtatcaagt acgcgcttgt agtagcgcgc ggcttcgaca tagtaaaagt
     6481 cacgcagctt gtcgtaggga cggtagttgg cctctttaaa gaggtcgtcc agaatccagg
     6541 ccgcgcggat ggcagagact gccttcttcg gagttggcgc cacctggttg ccacgcaggt
     6601 ggtagcaatc gacgttcagc atgtagctga ttgcgccatg cacgatactg cggttctcac
     6661 ccatgtcata tttaccgaag aatgtctcga cgaccttctg gcggtggcta accgtgttct
     6721 tcagcgcgag aatttcttct gcgttcaggt tggtaaagtc cttgggaaat gcggcaaaga
     6781 ggcacttggg acagacctgt acgtggtaaa cgagcgggtt aattttcccc cacttcttgt
     6841 tgtcttcgta caggcgttgg agctcctgcg tgagcttacc agcaatcagg cgcccgctac
     6901 ccgaaagcaa ttcttcttta aagtgtgttg tgccgcaaac agggcatttc gtcgcctctt
     6961 tggcgcgata agagattttc ttctggtt
//
LOCUS       DAOQJF010000166         6983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6007_length_6983_cov_6.010537, whole genome shotgun sequence.
ACCESSION   DAOQJF010000166 DAOQJF010000000
VERSION     DAOQJF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_6983_cov_6.010537"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..438)
                     /gene="rpsK"
                     /locus_tag="PLF85_09695"
     CDS             complement(19..438)
                     /gene="rpsK"
                     /locus_tag="PLF85_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNE19982.1"
                     /translation="MSEEKKEQTATAEEGKAKKGKDKGRVPRGRVYINATFNNTIITI
                     TDMNGNSIAWASGGSLEFKGSRKATPYAAQLAARSALDKARERGLHEVEIYVKGPGVG
                     REQAIRTLAASGLRVSLIKDITPVPHNGCRPRKKRRV"
     gene            complement(460..843)
                     /gene="rpsM"
                     /locus_tag="PLF85_09700"
     CDS             complement(460..843)
                     /gene="rpsM"
                     /locus_tag="PLF85_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNE19983.1"
                     /translation="MARIEGIDLPNQKRVVIGLTYIYGIGDKAAQDIVAKAGIPLTKR
                     VHELSEDEVGKIKKIIDADYTVEGALRSKVQLDMKRLVDIGSFRGSRHRKGLPARGQR
                     TKTNARTRKGRRVTVAGKKQAPSKG"
     gene            complement(847..963)
                     /gene="rpmJ"
                     /locus_tag="PLF85_09705"
     CDS             complement(847..963)
                     /gene="rpmJ"
                     /locus_tag="PLF85_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNE19984.1"
                     /translation="MKVRASVKKICPQCKIIRRAGIIRVICKSNPRHKQRQR"
     gene            complement(960..1796)
                     /gene="map"
                     /locus_tag="PLF85_09710"
     CDS             complement(960..1796)
                     /gene="map"
                     /locus_tag="PLF85_09710"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNE19985.1"
                     /translation="MAVRIYTSKEVSQIMESGMIAFETHMRLKDHIRPGVSTAELDEV
                     AREYIYSRKAKPAFLGLYGFPACICACVNDAVVHGIPDEKTVVREGDVLSIDLGVSYN
                     GYFSDTAWTWPVGEVSNEARQLITVTQQCLFKGIAEAQVDNRIGAIGEAVQKHAEANG
                     FGVVRQLVGHGIGRAIHEEPQVPNFGRRKEGIKIRAGMVLAIEPMINAGTYDVYTDSD
                     KWTVKTKDGKLSAHFEHTIAVTSTGPVICTMPKGANVNVFELMGHESAKVADVELAGV
                     SA"
     gene            complement(1798..2361)
                     /locus_tag="PLF85_09715"
     CDS             complement(1798..2361)
                     /locus_tag="PLF85_09715"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNE19986.1"
                     /translation="MQIIFLGPPGAGKGTQAKIFLDRAGIIQISTGDILRSAVANGTA
                     LGKQAKAFMDKGELVPDSVVIGIIEQRIQEPDCKKGFVLDGFPRTIEQAKALDLILEK
                     LGLNIDHVVNFEVPDEELVRRLLGRAAQEGRSDDNPESIKNRLQIFKDKTQPLINYYE
                     QKQKLRHIAGLGSTTEIADKVKAVVGV"
     gene            complement(2368..3744)
                     /gene="secY"
                     /locus_tag="PLF85_09720"
     CDS             complement(2368..3744)
                     /gene="secY"
                     /locus_tag="PLF85_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNE19987.1"
                     /translation="MVRALVTIFTVPDLRKKILFTLGMLLLFRLGAHITVPGLDFKVL
                     QDYFSKAAAGQGGNLTDYIDLFAGGAFKRLSVFALGIMPYISASIIMQIMMVAVPSLQ
                     RLAKEGDFGRRKINQYTRYGTVLLCALQAYGITVYITSLHFEALQGGKGMLIPGGPGA
                     AFTAMTMLTITAGTILLMWMGELMTERGIGNGTSLIIFAGIIAGAPAALGQFLGDIKN
                     NQADAVLALILLVLFIAMIGFSILLSEGQRKIPLQYGKRIVGRRMVQGASQTLPIKVN
                     AANVMPIIFASSLMIFPSQIASYFGSRYQGFAETIQKYLAPGTLTYFIIYILLILFFA
                     YFYTTIQYNPKEIAEALKKNGGYIPGVRPGAHTEETLERILNRITLSGAMFLAFVAIA
                     PDIFIKLWSLEKYSQLIHLFGGTSLLITVGVALDTLKQIESQLIMRHYDGFMDRNKKS
                     AGRPRVVR"
     gene            complement(3757..4248)
                     /gene="rplO"
                     /locus_tag="PLF85_09725"
     CDS             complement(3757..4248)
                     /gene="rplO"
                     /locus_tag="PLF85_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNE19988.1"
                     /translation="MNELKPNAGANKRRKIVGRGTSSGHGKTSGRGGKGQTARNGGNI
                     PARFEGGQWPLYRRIPKRGFQNLFRVEYTPVNIGNLARTVAAGKLPEAAVDLLSLKKA
                     GLLPKTAEAWKLLGKTDGKGVSLSGKKIVVHAVSEAARKLAESAGATVEVIESKPKAL
                     KKD"
     gene            complement(4248..4427)
                     /gene="rpmD"
                     /locus_tag="PLF85_09730"
     CDS             complement(4248..4427)
                     /gene="rpmD"
                     /locus_tag="PLF85_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNE19989.1"
                     /translation="MKKVRVTQTRSATRKSDKQQATLVALGLRRIGMSREHVVTPQLE
                     GMLKKVGFLVKVEAL"
     gene            complement(4438..5019)
                     /gene="rpsE"
                     /locus_tag="PLF85_09735"
     CDS             complement(4438..5019)
                     /gene="rpsE"
                     /locus_tag="PLF85_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNE19990.1"
                     /translation="MLDIDGQTIELTERVVSMARVAKVVKGGRRFSFNALTVVGDGQS
                     YVGLGFGKAKEVPEAIRKSIEDAKKNVMKIRKKGNTIPHEVIGRFKSARVMLKPGAPG
                     TGIIAGDSVRAVVELAGVHDILTKALGSSNPLNIVKATLDGLRQLGGIDEAKLRRGIP
                     LPVLFGNYSKQRRDEKRKALAGAVAPAANTPAN"
     gene            complement(5107..5493)
                     /locus_tag="PLF85_09740"
     CDS             complement(5107..5493)
                     /locus_tag="PLF85_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNE19991.1"
                     /translation="MASKARLNRQKTKRIARVRANLRKGNRSGKARIYFHVSGRHLYA
                     QVLDDVAGKTLLTCSTLDKAVQTGGKKSFTNKEFSAKLAETLVGKMQAANLKLDAGYV
                     FDRGAKLYHGRVKSFADTLREKGVSI"
     gene            complement(5506..6048)
                     /gene="rplF"
                     /locus_tag="PLF85_09745"
     CDS             complement(5506..6048)
                     /gene="rplF"
                     /locus_tag="PLF85_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNE19992.1"
                     /translation="MSRIGNKPIEIPAGVTITAAGDSVTVKGPKGSLQAPLFPGLTVE
                     VTGSTAKVSRANDEKDLRAKHGLVRSLVNNCVVGTSTGYSQVLILQGVGYRAQKKGKD
                     VVFSLGFSHEVVLKEPADVTVDVLENTKLKVSGIDKQRVGQIAAQIRGFKEPEPYKGK
                     GIRYENEQVRRKAGKAGSKK"
     gene            complement(6055..6447)
                     /gene="rpsH"
                     /locus_tag="PLF85_09750"
     CDS             complement(6055..6447)
                     /gene="rpsH"
                     /locus_tag="PLF85_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNE19993.1"
                     /translation="MTNDLIADMLTRLRNASAAGHESVEVRINNTCEKILGILKNEGF
                     LDSFVVYKSEATRMAKVELRYYQNKPVFSAIERVSKPGRRIYTPWKEIKPTRNNMGLS
                     IFSTPKGVLSEKEAKFQHVGGEFLCRVY"
     gene            complement(6455..6640)
                     /locus_tag="PLF85_09755"
     CDS             complement(6455..6640)
                     /locus_tag="PLF85_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNE19994.1"
                     /translation="MAKKSMQIKASRKPKFTVRGYNRCPLCGRAHGYMRRFEMCRICF
                     RENASRGLIPGVSKSSW"
     gene            complement(6659..>6983)
                     /gene="rplE"
                     /locus_tag="PLF85_09760"
     CDS             complement(6659..>6983)
                     /gene="rplE"
                     /locus_tag="PLF85_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803706.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNE19995.1"
                     /translation="ISNFKLREGLPIGVSVTLRGDRMYEFFDRLINIALPRVRDFNGL
                     SAKSFDSRGNYSLGIKEQIIFPEINFDDVEQIHGMDITLVIQSESPEHSAKLLEKFNF
                     PLRKK"
BASE COUNT         1619 a         2034 c         1556 g         1774 t
ORIGIN      
        1 ataaatctcc gtttcccctt ataccctgcg cttcttacgc ggccggcaac cgttgtgcgg
       61 aaccggtgta atgtctttga tgagactgac gcgaagtcct gaggcagcca gtgtcctgat
      121 ggcctgctcg cggcccactc ccgggccctt cacgtagatt tcaacttcat gcaggccacg
      181 ttcgcgtgct ttatccagcg cggaacgggc cgcaagctgc gctgcgtatg gcgtcgcttt
      241 acgcgagccc ttgaattcca gtgaaccacc cgatgcccag gcaattgaat tgccgttcat
      301 atcagtgatc gtaatgatgg tattgttaaa ggttgcgttg atatagacac gaccgcgggg
      361 cacccgtcct ttatctttgc ctttcttggc cttgccttct tcggcagttg cagtttgctc
      421 ttttttttct tcgctcattt tatgattcta ccttaaatat taacccttgc tcggagcctg
      481 tttcttgcct gcaaccgtca cgcgacgtcc tttacgcgta cgcgcgtttg tttttgtccg
      541 ctgcccacgc gcaggaagcc ctttacggtg ccgggaacca cggaagctgc cgatatccac
      601 caggcgcttc atatcgagct gaactttcga gcgcagagca ccttcaacag tatagtcagc
      661 atcaatgatc ttcttgatct tgcccacttc atcttctgaa agctcatgaa cccgcttagt
      721 aagcggaatt ccggctttag caacaatgtc ctgcgcagcc ttgtcgccga tgccgtaaat
      781 atacgtcaac ccgatgacaa cccgcttctg gttaggaaga tctatacctt caatacgtgc
      841 cattttttac ctctgcctct gcttatgccg cgggttcgat ttgcaaatca cccggataat
      901 tcctgcccgg cgaatgattt tgcactgcgg gcaaatcttt tttactgatg ctctgacttt
      961 catgcgctca ctccagctaa ctcaacgtcc gcgactttcg cggactcatg gcccataagt
     1021 tcaaatacat tgacattcgc acctttgggc atcgtgcaaa tgacaggccc cgtactcgtg
     1081 accgctattg tgtgttcaaa atgcgcgctc agcttgccat cctttgtctt tacggtccac
     1141 ttatcggaat ccgtatagac atcgtaggtg cctgcattga tcattggctc tatagccaaa
     1201 accatgcctg cgcgaatttt aattccctct ttccgccgac cgaaatttgg cacctgcggt
     1261 tcttcatgaa tcgcccggcc aatcccgtgt ccgacgagtt gccttactac accaaaacca
     1321 ttcgcttcag cgtgcttctg caccgcctca ccaatcgcgc caatgcgatt gtctacctgt
     1381 gcctcagcta tgcccttaaa caggcactgc tgggtaacgg taatcagctg ccgggcctca
     1441 ttcgaaacct cgcccaccgg ccatgtccat gcggtgtctg agaaatagcc attatagcta
     1501 acgcccagat cgatgctcag tacatcccct tcacggacaa ccgtcttttc atccggaatg
     1561 ccgtgcacta cagcgtcatt gacgcaggca cagatgcaag ccggaaagcc atacaatccc
     1621 agaaaagcgg gcttcgcctt ccgggaatat atatactcgc gagccacctc gtcgagttcg
     1681 gccgtagaaa cccccggacg aatgtggtct ttcaggcgca tatgagtctc aaacgcgatc
     1741 ataccggatt ccatgatctg cgagacttcc ttacttgtat aaatgcgcac cgccacgtca
     1801 taccccgact acggccttga ccttatctgc gatctccgtt gtactgccaa gaccagcgat
     1861 atgacgcagt ttctgctttt gctcgtagta gttgataaga ggctgtgtct tatccttaaa
     1921 aatttgcaag cgatttttta tggattccgg gttatcgtca gaccggcctt cctgggcagc
     1981 cctgcccagt agcctccgca caagctcctc gtcgggcacc tcaaagttga ctacatggtc
     2041 aatattcaat cccagctttt ccaggatgag gtccagagcc ttagcctgct cgatagtcct
     2101 cggaaaaccg tcgagtacaa accctttctt gcagtccggc tcctgtatac gctgctcaat
     2161 aatgccgatc acaacagaat caggcacaag ctcgcccttg tccatgaaag cctttgcctg
     2221 ctttcctaaa gctgtgccat tagctacagc cgagcgcaga atgtcgccag tagagatctg
     2281 gataataccg gcacgatcaa gaaatatctt ggcctgcgtg ccctttccgg ctcctggagg
     2341 tccgaggaaa atgatctgca tagggcctta ccgtaccacc cttgggcggc cggcgctttt
     2401 cttattgcgg tccatgaagc catcataatg ccgcatgatc aactggctct cgatctgctt
     2461 cagcgtatcc agcgcgacac ccacggtaat gagcagcgat gtaccaccga acaggtgaat
     2521 caactgcgaa tacttctcga ggctccaaag cttgataaaa atatccggag caatcgcgac
     2581 gaatgccagg aacatcgccc ctgaaagcgt aattctgttc aagatgcgct cgagcgtctc
     2641 ttccgtatgc gcacccggtc tcacacctgg gatataacca ccgttcttct tcaacgcttc
     2701 ggcaatctcc ttagggttgt actggattgt ggtgtagaaa tacgcaaaga acaggataag
     2761 gagtatatag ataatgaaat acgtcagcgt acccggagcg aggtacttct ggatggtctc
     2821 agcaaaaccc tggtagcgtg agccaaaata gctcgctatc tgcgatggaa aaatcatcaa
     2881 agatgacgca aaaataatcg gcatcacatt agccgcgttt accttgatgg gcagcgtttg
     2941 gctcgctccc tgaaccatcc ggcgcccgac aatacgctta ccgtactgaa gggggatctt
     3001 gcgctgaccc tcgctgagca ggatagaaaa cccgatcatc gcgataaaaa ggacaagaag
     3061 aatcagagca agtacagcgt cggcctggtt gttcttgatg tcgcccagaa actggccaag
     3121 agccgcaggg gcacctgcaa taatacctgc aaaaataata agagaggtac cgttaccaat
     3181 accgcgctct gtcatcaact cacccatcca catcagaagg atcgtccccg ctgttatcgt
     3241 cagcatggtc atcgcagtaa aagcagcacc gggacctccg ggaatcagca taccttttcc
     3301 gccctgcagt gcttcaaagt gcagcgaggt aatgtaaaca gtgataccgt acgcctgaag
     3361 ggcgcaaagc agcacggttc cgtaacgtgt gtactggttg atcttgcgac ggccaaaatc
     3421 gccttctttc gcgagccgct ggagcgatgg tacagccacc atcatgatct gcatgataat
     3481 cgatgccgaa atataaggca tgattcctaa agcaaatacc gaaaggcgct taaaggcacc
     3541 accagcaaaa agatcgatat aatccgttag gtttccaccc tgaccggcgg ccgctttgct
     3601 gaagtaatcc tgcagcactt tgaaatcgag gcctggaacc gtaatgtgcg caccaaggcg
     3661 gaacagcaag agcataccaa gggtaaacag aatcttctta cgaagatcag gtaccgtgaa
     3721 gattgttacc agtgctctta ccatgggaat ggatccttaa tcttttttca gagccttcgg
     3781 ctttgattcg ataacttcta ccgttgcacc agcggactct gccagcttgc gagcagcttc
     3841 gctgactgca tggacaacaa ttttcttgcc tgacagggat acaccctttc cgtcggtctt
     3901 gcccaagagc ttccaggctt ctgccgtctt aggcagcaac cccgctttct tgagactcaa
     3961 caaatcaact gcagcttcag gcaatttgcc tgctgcaaca gtgcgcgcca ggttaccaat
     4021 attcacagga gtgtactcaa cacgaaacag gttttggaaa ccgcgctttg ggatacgacg
     4081 atacagtggc cactggccgc cttcaaatcg cgcaggaata ttaccaccgt tacgggctgt
     4141 ctgccccttt ccaccccgac ccgatgtctt gccgtgaccg ctactcgttc cgcggccaac
     4201 aattttgcgg cgcttattcg caccggcatt aggtttcagt tcgttcatta taatgcctct
     4261 actttgacca gaaatccaac ctttttaagc ataccttcaa gctgaggcgt caccacgtgc
     4321 tcacgcgaca tcccgatgcg acgcagccca agggccacaa gcgttgcctg ctgcttgtct
     4381 gatttgcgcg ttgcgctgcg tgtttgcgtt acccggacct ttttcatact tcttacctca
     4441 attcgccgga gtatttgcag ccggtgctac tgccccggca agtgccttgc gcttctcatc
     4501 cctacgctgc ttagaatagt ttccaaacag aaccggaaga ggaataccac gacgaagctt
     4561 tgcctcgtct ataccaccga gctggcgcaa gccgtccagt gttgctttaa caatgttcaa
     4621 cggattcgat gaccccagtg ctttagtgag aatatcatgc acacctgcga gctccactac
     4681 agcacgaacc gagtcaccgg caatgattcc ggtacccggc gcaccaggct tcagcataac
     4741 acgcgctgac ttgaaacgac cgataacctc gtgcggaatg gtgttgcctt tcttccggat
     4801 cttcatgacg ttcttcttgg catcttcgat tgatttacga atagcttccg gaacttcctt
     4861 ggcttttcca aaccccagac caacgtacga ctggccgtca ccaactacag tcagtgcgtt
     4921 gaatgagaac cggcggccac ccttgacaac ctttgctacg cgggccatac ttacaactcg
     4981 ttccgtcagt tcaatcgtct gaccatcaat atcgagcaga ttcttcttca cgccctcttt
     5041 tggcccgcct ttcttgcggc ccggcttctt gcctgattgt tgttttgatt cttccataat
     5101 tacctgttag atactcacac ctttctcgcg cagcgtgtct gcaaaactct tcacacgtcc
     5161 atgataaagc tttgcaccgc gatcaaagac ataacctgca tcgagtttta ggttcgcagc
     5221 ctgcatcttg ccaaccagtg tctcggccag ctttgctgaa aattccttgt tagtaaaaga
     5281 cttcttgcca ccagtctgaa ctgccttatc cagtgttgaa cacgtcagca gggtctttcc
     5341 tgcgacatca tccaacacct gtgcatacag atgccgccct gacacgtgaa agtaaatccg
     5401 cgcctttccg ctgcggttac ccttgcgaag gtttgcccgc acccgggcta tccgcttcgt
     5461 cttttgcctg ttaagtcttg ccttagatgc catatttacc tgctgttact tcttgctgcc
     5521 ggctttacca gccttccggc gtacctgctc attctcatag cggatacctt tacctttgta
     5581 tggctctggc tccttgaacc cgcgaatctg cgctgcgatc tggccaacgc gctgcttgtc
     5641 tataccgcta accttcagct tggtattctc cagcacatct accgtaacat ctgcaggctc
     5701 tttgagcaca acctcgtgcg agaaaccgag gctgaataca acgtccttac ccttcttttg
     5761 cgccctgtaa ccaacaccct ggagtatcag aacctgtgaa taccccgtgc tggtaccaac
     5821 tacacagttg ttcaccaggg agcgaaccaa gccgtgcttt gcacgcaagt ccttctcatc
     5881 atttgcacgc gataccttag ccgtgcttcc ggtcacttcg acagtgagcc ccggaaacag
     5941 aggtgcctgt aaagaaccct tgggcccctt cacagtaaca ctgtcccctg cagcggtgat
     6001 tgtcaccccc gctggaatct caatcggttt attgccaatt cgtgacatga tacctcaata
     6061 gacgcggcag agaaattctc caccgacatg ctggaacttc gcttctttct cagacaacac
     6121 acccttcggg gttgaaaaaa tactcagacc catattatta cgggttggct tgatttcttt
     6181 ccaaggggta taaatgcgcc gacccggctt agaaacgcgc tcaatggcag aaaacacagg
     6241 cttattctgg tagtagcgga gctccacctt agccatgcga gttgcctcgc tcttgtacac
     6301 cacgaatgaa tccaggaagc cctcgttctt gaggattccc aggattttct cgcatgtatt
     6361 attaatacgc acttccacgc tctcatgccc ggccgccgaa gcgttccgta gacgtgtcaa
     6421 catatctgca atcaggtcgt ttgtcataat tttctcacca agatgatttg ctcacgccgg
     6481 ggataagacc acgcgacgcg ttttcacgaa aacaaatacg gcacatttca aaacgccgca
     6541 tataaccatg ggcacggcca cacagcgggc agcgattata gccacgaacc gtaaactttg
     6601 gcttccggct cgcctttatc tgcattgatt tttttgccat ctatcgttcc gtccctattt
     6661 acttcttccg cagcgggaaa ttgaatttct cgagcagctt cgctgaatgc tccgggctct
     6721 cagattggat caccagagta atatccatac cgtggatctg ctccacatca tcaaagttta
     6781 tctctggaaa gataatctgc tctttgatgc cgaggctgta atttccgcgc gagtcaaatg
     6841 acttcgcgct caatccatta aaatcccgaa cccggggcaa tgcaatgttg atcagtcggt
     6901 caaaaaattc gtacatacga tcgccacgca gcgtaacgct cacgccgatc ggcaaacctt
     6961 cgcgcaattt gaagttagaa ata
//
LOCUS       DAOQJF010000167         6950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6043_length_6950_cov_6.495431, whole genome shotgun sequence.
ACCESSION   DAOQJF010000167 DAOQJF010000000
VERSION     DAOQJF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6043_length_6950_cov_6.495431"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLF85_09765"
     CDS             complement(<1..842)
                     /locus_tag="PLF85_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE19996.1"
                     /translation="MRFLPLSLYALAESGIFAVEFFVRLQLLIFYTDRLGVESYLASL
                     AAGLALVWDAFIDPYVGAFSDRFRSRWGNRKPFLVAGALLIVPSLLWIFAPYREAGAI
                     GIFVQLLLASLLLNTGITLVTVPHAAISAELSKDAGVRVRIFAARLLFANLGLILGLI
                     IPAAMLNNARAVGIAETTVFPQIATVIGLFSLAAVLVCVASLQNTQPGEPGHKRRFVS
                     DLKTAVGNKFFLWLFAAGFLAYIGVAINSTLARYYYDHYLKINEDSLALVLIVFILVW
                     SLSVV"
     gene            complement(842..1615)
                     /locus_tag="PLF85_09770"
     CDS             complement(842..1615)
                     /locus_tag="PLF85_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE19997.1"
                     /translation="MDRLNLIATPWQQRFQEATHNLGGVSRAFAAAMVVSLGPGIEAA
                     EDVVRFRGDAYDLATGKFVYSENHSEYRKAGVHVYSRVSYRDRSGKEFANKLITFQGN
                     KMQPTYELRDARDGYVEGIRREGGQTVYFARRKADQPLQSKAITTPQPAVFDAGFDYF
                     VRENFEKICAGQNAAFNFAVPVELDYFRFRVSRQEGGEVCRMNLELDNFVLRQLVKPI
                     KLWYDTKERRLRKYEGISNINGPDGKSLKVRVVFDYTVK"
     gene            complement(1554..2837)
                     /locus_tag="PLF85_09775"
     CDS             complement(1554..2837)
                     /locus_tag="PLF85_09775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNE19998.1"
                     /translation="MKFYRKLFKYGLSKVEHGSLRVREAFPGGEDYVLGGKGPGYQAE
                     ISIVDPYFYKHCVLYGEIGFGEAYVARSWETEDLNLALRWFAQNAERMPGFSGSSATT
                     WFLNILGFVNRVKHYLRPNTMKISRKNISDHYDIGNPLYELMLEKTMAYSCGIFRNER
                     ESLDVAQERKFEALCRKLQLKKSDHLLEIGSGWGGFAVYAAKKYGCRITTVTISEQQY
                     NYAKEKIRKAKLDKQIDLRLADYRTLEGTFDKIVSIEMAEAIGFKYFDTYFGQLSRLL
                     KQDGLIALQYITFPESRFEQYLKNTDFSQIYIFPGSCLLSNLEVMKSLHRTSDLLLQD
                     LETMGQHYVTTLRKWRLNIEKNKSKVLKMGYDEKFFRKWVYYLAFCEAGFAERAINDV
                     QVVLGRANQTSYGDYNGQTEPYRDPLAAAVSGGNA"
     gene            complement(2834..3595)
                     /locus_tag="PLF85_09780"
     CDS             complement(2834..3595)
                     /locus_tag="PLF85_09780"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNE19999.1"
                     /translation="MLEGIYETEVMHARQKPARQIFGVRGYSLCVDVARFDGVGAPLR
                     LLSFTRPAWHRFRRSDFSLLADKSHSAVGCAAKFLREKTGIVADRFFLLAHPAVAGYI
                     FNPVSFFFCLAGERHVATIVEVNNTFGEQKHFVLPGSETRARARKNFYVSPFISPFAD
                     FAMKVLLPAEKLDITINTMRAESPELLARMSGTRFALSDMQLLKFSLKYPFYTIKVIV
                     LIHYYALRLFIKGVPFFPKKNTDAAIMHTELRSSR"
     gene            complement(3595..4851)
                     /locus_tag="PLF85_09785"
     CDS             complement(3595..4851)
                     /locus_tag="PLF85_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE20000.1"
                     /translation="MKKLAIVGSGVAGLGAAWLLRDKYDITIFEAGKYAGGHTNTLDV
                     PLPQGGTQAVDTGFIVFNEHTYPHLLDLFRQLGVEYANSDMSFGHFNTNTGLQWCSRG
                     LGGLFAQRKNLFSPRYYRFLLEANRFNSQLPKDLEAGRVEGSFGDYLKRNGFSDFFAE
                     NYIVPMTAAVWSTPPERMLAFPARTFARFFVNHGFLSLYSGLQWKYVVGGSRSYVKKI
                     LAGFKGKLYLESPVVRVEQDPDGVTVHLKGEKQRFDGCLIASHADQTLKMLGNATEQQ
                     RELLSQFRYEPNLAVLHSDEKVMPPIRKTWSSWNFKLGKNRNGEMKSTVIYYMNLLQR
                     IPGNIPYFVSLNDISAIDPEKTYARIEYEHPLFDEQTEKAQAELPQLNEDGKIFFSGS
                     YFRYGFHEDAYMSAVQAAEAISRRLV"
     gene            complement(4848..5642)
                     /locus_tag="PLF85_09790"
     CDS             complement(4848..5642)
                     /locus_tag="PLF85_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNE20001.1"
                     /translation="MRLTAKKLSELTSVPAANIRKWRERYSILDPQRGDNGYLYYTSD
                     DYRVVLSISRLLLEGKKLSSIMQLGRDHLLSIAPESSYTPEEMRFLEYVIEGDFGTLS
                     REFDLYYRTHTMDSLIQKKVWPYTVLVGKAWESGFITVATEHAFSRWIMGYLSQLALT
                     FTRKKGGERLFIAFPGDLHEIGAMMHYVLMASQGMGGKFCGALPVEKLFEELSHGDYD
                     ELHVSATVPRTREEIEKFVSQIHKKYPRIKVKIGGTGARSKEGISA"
     gene            complement(5639..6664)
                     /locus_tag="PLF85_09795"
     CDS             complement(5639..6664)
                     /locus_tag="PLF85_09795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNE20002.1"
                     /translation="MIDSLLAKGFLPDYVIRKGIRALNRRRLKEISADNIELGQAKAM
                     ALVEQLRQSKIAILTEKANEQHYEIPAEFFALVLGKNRKYSSCYYPTGKETLDQAEDL
                     MLAETVAMARLENGQEILELGCGWGSLSLYMAKNFPKSRITGVSNSASQKAYIDGEAK
                     KRGIRNLKIITADVNEIKPGKSFDRVVSVEMFEHMRNYDQLFARVRSWLKPKGMLFVH
                     IFTHRMFPYLFEDKAKDDWMSRYFFSGGIMPSNFLFSYFSKGFSLKEQRIYSGTHYHL
                     TSEHWLQNMDKHRAEIEQIFAGVYGKENVTRWINYWRVFFLAVSELFRTRGGEEWNVS
                     HYLFEKV"
     gene            complement(6735..>6950)
                     /locus_tag="PLF85_09800"
     CDS             complement(6735..>6950)
                     /locus_tag="PLF85_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20003.1"
                     /translation="PEFYAMGWYVLPGRQTVSHDGNFNGYHTMNWMQLKKRQAIILLS
                     NRYTTKIREITGEIDRALNGLEALELR"
BASE COUNT         1673 a         1897 c         1773 g         1607 t
ORIGIN      
        1 acaacagata gggaccaaac caggataaaa acaatcagca ccagcgcgag actgtcttcg
       61 ttgattttca ggtagtggtc ataatagtag cgcgccagcg tcgaattaat cgccacgccg
      121 atataggcga gaaatcctgc ggcaaaaagc cagaggaaga acttgttacc gactgccgtt
      181 ttgaggtcgc tcacgaacct ccgcttatgg ccgggctcac cgggctgcgt attttgcaga
      241 gaagccacgc agacaagcac agcagcgagc gagaacaagc cgatgactgt ggcaatctgc
      301 ggaaagaccg tggtttcggc gatcccgacc gcccgggcat tgttgagcat cgctgcagga
      361 ataataagtc cgagtatcag gccgaggttt gcgaagagca ggcgcgcggc aaaaatgcgt
      421 acacgcacgc ctgcatcttt cgaaagttcg gcagatattg ccgcatgcgg cacggtgacg
      481 agcgtgatac ccgtattgag cagcaggctt gccagcagca gctgcacgaa tatacctatc
      541 gctcccgctt cgcggtaagg cgcgaatatc cagagcagcg acggaacaat caacagggcg
      601 ccggcaacca ggaaaggttt cctgtttccc cagcggctgc gaaaccggtc agaaaaagcg
      661 ccgacatagg ggtcgataaa ggcgtcccac acgagggcaa gccctgcagc cagcgacgca
      721 aggtaactct cgacaccgag acgatcggtg taaaaaatca gcagctgcag gcgaacgaaa
      781 aattcgacgg caaagatacc cgattcggca agggcgtaaa gagagagtgg taagaagcgc
      841 attattttac cgtgtagtcg aaaacaaccc gcaccttcag gctcttcccg tcggggccgt
      901 tgatattcga aatgccttca tacttgcgca accgccgctc tttcgtgtcg taccagagtt
      961 tgatgggttt gaccagctgg cgcagcacaa agttgtcgag ctcgagattc atgcggcaaa
     1021 cttctccgcc ttcctgccgc gaaacacgga agcggaagta atcgagttca accggcaccg
     1081 caaaattgaa tgcagcattt tgcccggcgc agatcttctc gaaattttcg cgcacgaaat
     1141 aatcaaagcc ggcgtcgaac acagcgggct gcggagttgt tatcgccttc gactgcagcg
     1201 gctggtctgc cttgcgccgg gcaaaataaa ccgtttgccc gccttcgcgc ctgataccct
     1261 caacgtaacc gtcgcgcgca tcgcgcagct catacgttgg ctgcatcttg ttcccctgga
     1321 aggtgatcag cttgttcgca aactctttac cgctgcggtc gcggtacgaa acgcgcgagt
     1381 agacgtgcac tccggccttg cggtactcgg agtgattttc ggaataaaca aatttgccgg
     1441 ttgccaggtc atacgcatca ccgcgaaagc ggacaacgtc ttctgcagct tcaatacctg
     1501 gtcccagcga aacaaccatg gcagcagcga aagcccggct cacgccaccg aggttatgcg
     1561 ttgcctcctg aaaccgctgc tgccaggggg tcgcgataag gttcagtctg tccattgtaa
     1621 tctccataac tcgtctgatt tgcccggccc agaaccacct ggacatcatt gatcgcgcgt
     1681 tcagcgaagc ccgcttcgca gaacgcgaga taatagaccc acttccgaaa gaatttctcg
     1741 tcgtaaccca ttttgagcac tttacttttg ttcttctcga tattcaaccg ccacttgcgc
     1801 aaagtcgtta cgtagtgctg tcccatcgtt tccaggtctt gtagcaacag gtctgaagtg
     1861 cggtgcagtg atttcataac ttcaagattc gacagcaggc aagaccccgg aaaaatatag
     1921 atctgcgaga aatcagtatt tttcaggtac tgctcgaaac gcgactcggg aaaggtgata
     1981 tactgcagcg cgatgagacc atcttgtttt aacagcctcg acaactggcc gaagtaggta
     2041 tcgaagtatt tgaaaccaat ggcctcggcc atttcaatcg atactatttt gtcgaaagtt
     2101 ccttccagtg tccggtagtc ggccagacgc agatcaatct gcttgtcgag ctttgccttg
     2161 cggattttct ctttcgcgta attgtactgc tgctccgaaa tagtgacagt ggtgatgcgg
     2221 cagccatact ttttcgccgc gtaaaccgca aaaccacccc aaccgctgcc gatttcgagc
     2281 aagtgatcac tttttttcag ctgcagtttg cggcaaagcg cttcaaattt gcgttcctgc
     2341 gccacatcca gcgactcgcg ttcgttgcgg aaaataccac atgagtacgc catcgtcttt
     2401 tcgagcatga gttcatacag cgggttacca atatcgtagt gatcagaaat gttcttgcgg
     2461 ctgattttca tcgtgttcgg cctcaggtaa tgttttaccc ggttaacgaa tcccaggata
     2521 ttcagaaacc acgtggtcgc gcttgaaccg gaaaatcccg gcatacgctc ggcattctgt
     2581 gcgaaccagc gcagggccag gttgagatct tctgtttccc acgaccgggc aacgtaagcc
     2641 tctccaaaac caatttcacc atagagaacg cagtgcttgt aaaagtaagg atcaacaata
     2701 ctgatttcag cctggtaacc cggccctttc cctccgagta cataatcttc gccgccggga
     2761 aacgcctcac gtacccgcag cgagccgtgc tctacctttg aaagaccata tttgaacaat
     2821 tttctgtaaa acttcacctg ctactcctca gttcggtatg cataatcgcc gcatcagtgt
     2881 tcttcttggg aaaaaacggc actcccttga taaacagcct cagcgcatag tagtggataa
     2941 gtacaataac cttgatagta tagaacggat acttcaggga aaatttcagc aactgcatgt
     3001 cgctcagcgc gaagcgcgta ccgctcatgc gggcaagcag ctcgggactc tctgcccgca
     3061 tcgtattgat tgtaatgtcg agtttttccg ccggcagcaa aactttcatg gcgaaatcgg
     3121 caaacggaga gatgaacgga gaaacataga aattcttgcg cgcgcgggcc ctggtctcgc
     3181 tgcccggcag aacaaaatgc ttttgctcgc caaaggtatt gttgacttca acaatagtcg
     3241 ccacgtgcct ttctcccgcc aggcaaaaga agaagctgac tggattaaaa atatagcccg
     3301 ccacggccgg gtgcgcgagc agaaagaatc tatccgcgac aataccggtt ttttcccgca
     3361 ggaattttgc tgcgcagccg acagctgaat ggcttttgtc tgccagcagg gaaaagtcgg
     3421 atcgcctgaa ccggtgccag gcgggccggg taaaggaaag cagacgcagt ggagcaccga
     3481 ccccgtcaaa acgcgccaca tcgacacaga gcgaatatcc acgcacgccg aagatctgcc
     3541 gagcaggttt ctgccgggcg tgcattactt cagtttcgta gataccttcg agcatcagac
     3601 gagccgccgt gaaattgcct ctgccgcctg taccgcagac atataagcat cttcatgaaa
     3661 gccgtaccgg aagtagctgc cgctaaagaa gatttttcca tcttcgttga gctgcggcaa
     3721 ctcggcctgc gccttttctg tctgctcgtc gaacagcggg tgttcatatt ctatgcgcgc
     3781 ataggtcttt tcgggatcga tagcagaaat gtcattcagc gacacaaaat acggaatatt
     3841 accgggaatt ctctgcaaca agttcatgta gtatatgacg gttgatttca tttcgccgtt
     3901 gcgatttttg ccgagcttga agttccacga agaccaggtt ttgcggatcg gcggcataac
     3961 cttttcgtcg ctgtgcagca cggccaggtt gggttcataa cggaactgcg acagcagctc
     4021 acgctgctgc tcggttgcat tgcccagcat cttgagtgtc tggtcagcgt gagaggcaat
     4081 gaggcaacca tcaaaccgct gcttttcccc cttcaggtgc acggttacgc cgtcggggtc
     4141 ctgctcgacg cgcaccaccg ggctctcgag gtaaagtttt cctttgaaac cggcgagtat
     4201 cttcttcacg taactgcggc tgccgccgac aacgtatttc cattgcaggc cgctgtagag
     4261 gctcaggaag ccatggttga cgaagaagcg ggcaaacgtg cgcgctggga aggcaagcat
     4321 gcgctcgggt ggcgtcgacc aaacagccgc ggtcatcggc acaatgtagt tctctgcaaa
     4381 aaaatcgctg aacccgttcc gtttcaggta gtcgccgaat gaaccttcga ccctgcccgc
     4441 ctcaagatct ttgggcagct ggctgttgaa gcggtttgcc tcgagtaaaa agcgataata
     4501 gcggggcgag aaaaggttct tgcgctgggc aaacaatcct ccgaggccac gcgagcacca
     4561 ctgcagtccc gtattagtat tgaaatgacc aaaggacatg tcgctgttgg cgtattcaac
     4621 cccgagctgg cgaaagaggt cgagcagatg cgggtaagta tgctcgttga aaacgataaa
     4681 accggtatcg accgcctggg ttccgccctg cggcaggggg acatcgaggg tatttgtatg
     4741 acctccggca tattttcccg cttcaaagat ggtgatgtcg tatttatccc tcagtagcca
     4801 ggctgcaccg aggccggcca caccactgcc aacgatcgct aattttttca tgctgaaatt
     4861 ccttctttac ttctggcgcc agtgccgcca atcttcacct tgatgcgcgg gtatttctta
     4921 tgtatctggc ttacgaattt ctcaatttct tcgcgcgtcc gagggactgt cgccgatacg
     4981 tgcaattcat cgtaatctcc atggctcagc tcctcgaata gtttttccac cggcagggcc
     5041 ccacaaaatt taccgcccat accctgcgat gccatgagca catagtgcat catcgcgccg
     5101 atttcgtgca gatcaccggg aaatgcaatg aacagccgct cgccgccctt cttccgcgta
     5161 aaagtcagcg ccagctgcga caagtaaccc atgatccagc gggaaaaggc gtgttcggtc
     5221 gccacagtga taaaacctga ttcccaggct ttgcccacca gcaccgtata aggccagact
     5281 tttttctgta tcagcgagtc catggtatgt gtgcgataat acagatcgaa ctcgcgggac
     5341 agggttccaa aatcaccctc tatcacgtac tcaaggaaac gcatttcttc aggcgtataa
     5401 ctcgactcgg gggctatcga cagcaggtgg tccctgccca gctgcatgat cgaagagagt
     5461 tttttgcctt cgagcagcag ccggcttatg ctaagcacca cccggtagtc gtcggatgta
     5521 taataaagat aaccgttatc tccgcgctgc ggatcaagaa tcgagtaccg ctcgcgccat
     5581 ttacgaatat tggcggcagg taccgaagtc agctcactca gttttttagc cgtgagcctc
     5641 ataccttctc aaacaggtag tgactgacat tccattcttc gccgccgcgc gtgcgaaaga
     5701 gttctgaaac cgcgaggaag aacacgcgcc agtaattgat ccaacgcgtg acgttttctt
     5761 tgccgtaaac accggcaaag atctgctcta tttctgcgcg gtgcttgtcc atgttctgca
     5821 gccagtgttc agacgtcagg tggtagtggg tcccgctgta aatgcgctgc tctttcaggc
     5881 taaacccctt ggagaagtag gagaacagga aattagaggg catgattccg ccggagaaaa
     5941 agtaccgcga catccagtca tccttcgcct tgtcttcaaa caggtaaggg aacatgcggt
     6001 gcgtgaagat gtgaacaaac aacatgcctt tgggcttcaa ccaagagcgc acacgcgcga
     6061 acagctggtc atagttgcgc atgtgctcga acatctctac tgagacgacg cggtcaaaac
     6121 tcttgccggg tttgatctcg ttcacgtcgg ccgtaataat cttcagattc ctgatccctc
     6181 gctttttcgc ttcgccgtcg atataggctt tctggctggc agagttggaa acgccggtaa
     6241 tgcgcgactt gggaaaattt ttggccatgt agagagagag cgaaccccaa ccgcacccga
     6301 gctccagtat ttcctgaccg ttttcgagcc gggccatcgc gacggtctct gccagcatca
     6361 ggtcttccgc ctggtcgagc gtctctttac ctgtcgggta ataacagctg ctgtacttgc
     6421 ggtttttacc gagcacaagc gcaaaaaatt cagccggaat ttcgtaatgc tgctcgtttg
     6481 ccttttcagt gagtatggcg atctttgact gccgcagctg ctctaccagc gccatagctt
     6541 tcgcctggcc cagctcgata ttatcggcgc tgatttcttt caggcgacgg cggttgagtg
     6601 cgcgtatgcc tttgcgaatt acgtaatcgg gtaagaaacc ctttgctaac agcgagtcga
     6661 tcattgtcca atgatattac acaagcttgg acattgcgcc cacaaaaaaa cgcaaaagca
     6721 gcaaacgagt atctctaacg gagctcgagc gcctcaagcc cgttgagcgc ccggtcaatt
     6781 tcacccgtga tctcacggat tttagtcgtg tagcggttac tgagcagaat aattgcctgg
     6841 cgctttttca gctgcatcca gttcatcgta tggtaaccat taaaattacc gtcatggcta
     6901 accgtttgtc gcccgggcaa tacataccag cccatagcgt agaactcagg
//
LOCUS       DAOQJF010000168         6938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6063_length_6938_cov_5.674851, whole genome shotgun sequence.
ACCESSION   DAOQJF010000168 DAOQJF010000000
VERSION     DAOQJF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6063_length_6938_cov_5.674851"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLF85_09805"
     CDS             <1..688
                     /locus_tag="PLF85_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE20004.1"
                     /translation="EMRGLNILQASDLHIRNSGDAEWLRQKLSFFIKQYQPNRVVLTG
                     DLTADGSQEQWNLLQNALKEWEHVRYILGNHDMPLKEQDGELRGTTTYEDLGDLRLIY
                     LDSAWRGPFAGSYTSVPEAELKQLDAALNTHRRILLFAHHPIAKGSPHFVLRNAAQIR
                     GMFSGKQVIGVFTGHFHGAYQALEDNIFYGGVMPLSNHQRNHTWSQRKGIRLIEVANS
                     CLRSSHLLLD"
     gene            694..1446
                     /locus_tag="PLF85_09810"
     CDS             694..1446
                     /locus_tag="PLF85_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20005.1"
                     /translation="MRLAIALLCLICAGNAGAALVTTDSEAMPESKGRQVTNTFVFSP
                     ENRDTGWVFATGMHLTVIPKKVAESETRFFPLLEAYLNKDFLKYLSWRNSVGVLVIQN
                     YASTGLSFVYHFPWFSVSIGMNTVLWGGIFKSSGFDVITYTWVNNPSFSIGKNLRKYY
                     YWLPDSLSITMKLNRLNYQYAQLGNSAVEKRRADFEGYTIDIMAEYGTGTGGIYLGLR
                     INHARPGYQFWLAFSDYDYFMNYTTLYAGYRF"
     gene            1463..2713
                     /locus_tag="PLF85_09815"
     CDS             1463..2713
                     /locus_tag="PLF85_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNE20006.1"
                     /translation="MLTNKAICRTAVIWGLAILANQCSILTEEEDRSFGDQGLLKNGI
                     PLSDAQLAHLKGNYRVTITGTEKFSGPVSIVASNGRISLLSYERETYAVMNAGCVDNE
                     VVFEGRSYDPASQEGGLVRIRVENTTDSAQICAGNPPVSAHVAYVGVSGRTKNSLTNL
                     IKLEYVGPLKTISKYIIGHHGCRTVDNCGASENTLESIKLAGNLGANSVEIDVHMSKD
                     QVPILYHDDDFNSRLVSGIFCLGSVDNYTVLQMKVFCRLKYGERIPTLREALQALLTD
                     DTMKMVWLDPKKTSALGPMVALANEYKAKATALGKSLEILIGIPDDDYLSAYLASPGY
                     ENTACLIEYDYTKAMAYNCRAYGPRFTVGPQRQYADILHGAGKLIFYWTVNDKDYFRL
                     YFDEGAADGLISDRIFNIYTMLQQ"
     gene            2713..3417
                     /locus_tag="PLF85_09820"
     CDS             2713..3417
                     /locus_tag="PLF85_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20007.1"
                     /translation="MKNLKLSLLSGLLLAVPWSSGLFALRVPFPDVQVGTWAVWHYAV
                     KPQPRATSPGSKVRMGGTGFGFDATVRYFLPFYYGVQYEYMPVYSESGGVTLDGYGNV
                     AYGANLYAHYFAGAIYFPLGDWVYELFFESDAKPMPALWQSLLRSPYVKASYGLHHMY
                     VTLTTNGEDSTSHAARFGYTGEAGLMFMVLRNLRVYGAFDYHSVYEDDYALVGLKTGM
                     VYRYSFEAARLPDKDK"
     gene            3428..4498
                     /locus_tag="PLF85_09825"
     CDS             3428..4498
                     /locus_tag="PLF85_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20008.1"
                     /translation="MNRNVTNDLLKLICVCTTWAALGCNSWGRFWDPTQKPKITAYSI
                     PAINATGIITGNQISVIAPTLTALSPQVATFTTTGQSVTVAGVSQVSGTTSNDYTSPL
                     IYQITGADGATATYTVTLTAPRVYGGSTLRLWLRADSLALADGTDIANWNDESGTGNH
                     MAQATAGQRPIFRTNRVNGLAVASFTDAANSTMSGPDTNINDQSNGSFFIVMAVTANT
                     SLRNILTLHGPQGRAFDLTDGPPTSFIQGRNGAGYNFTSAFDFGLNNFIALGSVQVAA
                     ISVNEIWNGDLKTSSGLLYATYDTDGSTSNTNYISNGNLEAEIAEVMYFDAALSQTEQ
                     DKVFCYLNTKYNLVSARRVCDS"
     gene            complement(4495..6321)
                     /locus_tag="PLF85_09830"
     CDS             complement(4495..6321)
                     /locus_tag="PLF85_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNE20009.1"
                     /translation="MKPLFKEISFGLNAGDKKGLIAKNGAGKSTLMQILVGRESADAG
                     RVVLKNGITLAYLDQSDLLDESQTILETIFDSKKPVMRLLARYEEAMTTGRGIDAAIA
                     DVEAAGAWDYERKIKGILTRLGITNLGAKVADLSGGERKRVALAHALIEEADVLVLDE
                     PTNHLDLDMIEWLESYLQQDQITLLLVTHDRYFLETICDEILELDDGQLFAYQGNYSN
                     YLARKAEREEAEQASLEKAQNKMRRELEWIRKQPKARTTKAKFRVNAFEDLKVEATAR
                     KKESLALLETEISRVGGKILDIDNLSKKFGERQIVSDFTYHFLKGDRIGVIGRNGVGK
                     STFLNLITGHEQADSGKVEVGATTVFGYYRQQGPEFRAGMTILDVVRDVAEAIPLPKG
                     KSITAAQLLEQFLFPPATHRQQAATLSGGEKRRLYLLTILMKNPNFLILDEPTNDLDI
                     ATIAVLEEYLQNFPGCLLVVSHDRYFMDKLTQHLFVLDKDFRDTEEGVITDFLGNYSD
                     YLAAKEAKSRQVIQQEKEELRKERKPKERPKQITFAQKKERDQLEKEIPMLEARLESL
                     NAEMQAGGNFEKISELGRSIDELQKEIESKTARFLELLELPG"
     gene            6542..>6938
                     /locus_tag="PLF85_09835"
     CDS             6542..>6938
                     /locus_tag="PLF85_09835"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNE20010.1"
                     /translation="MQAVITEFKWEKKYSTGILEIDAQHQYLFALTNRLLRNVAEKVS
                     LATIGVTIAQLEDYVDEHFKREEAMLRRAKYESLDKHIEQHQKLRSQLGQYGSRLQDQ
                     SLSVTDFAHFMQLWLSKHILHEDMKYLPAV"
BASE COUNT         1607 a         1846 c         1813 g         1672 t
ORIGIN      
        1 cgagatgcgg ggcttgaata ttctacaggc cagcgatctg cacatccgga actccggcga
       61 cgccgagtgg ctcaggcaaa agctaagctt ttttattaag caataccaac ccaaccgggt
      121 tgttcttacc ggcgacctga ccgccgacgg ctcgcaggaa caatggaatc ttctgcagaa
      181 tgccctgaaa gaatgggagc atgtcaggta catactcggt aaccacgata tgccgctgaa
      241 agagcaagac ggcgaattgc gtggcacgac aacctatgaa gatttgggcg accttaggct
      301 tatctacctc gatagtgcgt ggcggggccc gttcgcgggt agttatactt cagttcccga
      361 agctgaacta aagcagcttg atgctgccct gaacacccat cggcgcattc tgctgttcgc
      421 gcaccacccc atcgccaaag gttctccgca ttttgtgctg agaaacgcgg cacagatacg
      481 ggggatgttt tccggcaagc aggttatagg ggtatttacc ggtcacttcc atggtgccta
      541 ccaggcattg gaggataata ttttctacgg tggcgtcatg ccactgagca atcaccagcg
      601 caaccatacg tggagccagc gcaagggtat tcgcctgatc gaagttgcga atagttgttt
      661 acgcagctcg catcttctgc tcgactgagt gccttgcgcc tggctattgc actcctttgc
      721 ctcatttgcg ctggtaacgc cggcgctgcg ctggttacca cagactccga agcgatgcca
      781 gaaagcaagg gccgccaggt aacgaatacc ttcgtcttta gtcccgagaa ccgggacacg
      841 ggatgggtct ttgcgactgg tatgcacctg acggtaatcc ctaagaaagt cgcagaatct
      901 gaaacacggt ttttccccct gctcgaagct tacctgaaca aggactttct gaaatacctg
      961 agctggcgca attctgtggg agtccttgtt attcagaatt atgcaagcac tggcttgtcg
     1021 tttgtttacc attttccatg gttcagcgtc agtatcggaa tgaatacagt cctgtggggt
     1081 ggaatcttca agtccagcgg gtttgacgtg atcacctata catgggtcaa caatccttca
     1141 ttctcgatcg gcaaaaatct gcgcaagtac tactactggc ttcctgatag cctctcgata
     1201 acaatgaaac tcaaccgcct gaattaccag tatgcgcagc taggtaattc tgctgtcgaa
     1261 aaaaggcgtg ctgattttga aggttatacg atcgatatta tggccgaata tggtacggga
     1321 acaggcggta tctacctggg gttgcgcatc aaccacgcac gtccgggcta ccaattctgg
     1381 ctggcttttt ccgattatga ctatttcatg aactatacta ctctctacgc aggctatcgc
     1441 ttctgacagg gggatcggga ttatgctaac aaacaaggca atttgtcgca ccgcagttat
     1501 ctgggggctg gcaatactgg ccaatcaatg ttccattctg acagaagagg aagaccgtag
     1561 tttcggcgac caggggcttc tgaagaatgg tattccgctt tcggatgcac agctcgccca
     1621 cctgaaggga aactaccggg taaccatcac gggcacggaa aagttctccg gtccggtatc
     1681 cattgtggct tccaatggcc gcatctctct gctttcttac gagcgtgaaa cctacgcagt
     1741 aatgaacgcc ggctgcgtcg acaacgaggt cgtatttgaa ggacgcagtt acgaccctgc
     1801 ttcgcaggag ggcggattgg tacgcatacg cgtagaaaac actacggata gcgcgcagat
     1861 ttgtgcggga aatccaccgg tgagtgcgca cgttgcctat gtcggcgttt ccggaagaac
     1921 gaaaaactca ctcaccaacc tcattaagct tgagtatgta ggtccgctaa agacgataag
     1981 caagtacatc atcgggcacc acggctgccg tacggttgat aactgcggtg cttcagaaaa
     2041 cactctcgag tcgattaaac tcgcgggcaa cctgggggca aactctgtcg aaattgacgt
     2101 gcatatgagt aaagaccagg tacccatatt gtaccacgac gatgacttta attcgcggct
     2161 tgtctcaggt attttctgcc tgggatcagt cgacaactat accgttttgc agatgaaagt
     2221 attctgccgc ctgaaatatg gcgagcgcat accgacgctg cgcgaagcgc tgcaggcgct
     2281 gcttaccgac gacaccatga agatggtttg gcttgaccct aaaaaaacct ctgcgctggg
     2341 ccccatggtt gcattggcaa atgaatacaa ggcgaaagca acagcgctgg ggaagagtct
     2401 cgagattttg atcggtatac ccgacgacga ttatctgagc gcgtacctgg cgtcacccgg
     2461 gtatgagaac acagcttgcc ttatcgaata tgactacaca aaagccatgg cgtacaactg
     2521 cagggcatac ggcccgcgct tcacagtcgg gccgcagcgg caatacgccg acatactgca
     2581 cggcgcaggc aagctgatct tttactggac cgtgaacgac aaagactact tcagactgta
     2641 ttttgatgaa ggtgccgccg acggcctgat cagtgaccgt atatttaata tatacacgat
     2701 gctccagcag tgatgaaaaa tctgaaactc agtctgcttt ccggcctgct tcttgcagta
     2761 ccctggtctt ccgggctttt cgcattgcgt gtgccgtttc ccgatgttca ggttggtacc
     2821 tgggcggtgt ggcactatgc agtcaaaccg caaccacggg cgacctcgcc ggggtctaaa
     2881 gtccgcatgg gtggtacagg attcggtttt gacgcaacgg tcaggtattt cctgccgttt
     2941 tattacgggg tgcagtatga gtacatgccg gtctactcag aatctggcgg agttaccctc
     3001 gatggatacg gcaatgtcgc ttacggggca aatctttacg cacattattt cgctggtgca
     3061 atctattttc cgctgggcga ctgggtttat gaactcttct ttgaatccga tgcaaaaccc
     3121 atgccggcgc tctggcagag cctgctgcga tcgccttatg taaaagccag ctatggcctg
     3181 caccatatgt acgtgaccct taccacaaac ggcgaagact cgacttctca tgccgcgagg
     3241 tttggctaca ccggagaagc cggcctcatg ttcatggttt tacgaaatct acgggtgtac
     3301 ggggcatttg attaccacag tgtttatgaa gatgattacg cccttgtggg gctaaaaaca
     3361 ggtatggtct atcgctattc attcgaagcc gcgcgcctgc cagataaaga taaatagtga
     3421 ggtaaagatg aaccgaaacg taactaatga cctgcttaaa ctcatctgtg tctgtaccac
     3481 ttgggcggct cttggctgca acagctgggg aagattctgg gatccgaccc aaaaacctaa
     3541 aattacggca tattcgattc cagcaatcaa cgcaacaggt atcatcactg gcaaccagat
     3601 cagcgttatc gctccaacac tgacagcgct ctcgccacag gttgcgacct tcaccacgac
     3661 aggccaaagc gtgacggtcg ctggtgtatc gcaagtcagt ggtacgacga gcaacgacta
     3721 tacgtcgccg ctcatctacc agattacggg tgccgacggt gcaactgcaa cctacaccgt
     3781 gacactgact gccccacgcg tctatggcgg cagcacgctg cgcctgtggc tgcgcgccga
     3841 ctcgcttgcg ctggcagacg gcaccgacat cgccaactgg aatgacgaaa gcggcacagg
     3901 caatcatatg gcgcaggcga ctgcaggcca gcgcccgata ttcagaacga atcgggtaaa
     3961 tggtctggca gtcgcgagtt tcaccgacgc cgccaattct accatgagtg ggccagacac
     4021 gaacatcaat gaccagagca atggttcatt cttcattgtc atggcggtaa cggccaatac
     4081 aagccttcgc aatattctga ctttgcacgg ccctcagggg cgagccttcg accttaccga
     4141 tggcccgcca acttcattta ttcagggacg caacggcgca ggatacaatt tcacatcggc
     4201 ttttgatttt ggactgaata attttatcgc cctgggcagt gtgcaggtag cggcaatcag
     4261 cgtgaatgaa atttggaatg gtgacttaaa gacatcgtca ggcttattgt acgcaactta
     4321 tgacaccgac ggcagcactt cgaacacgaa ttatatttcg aacggaaacc tcgaagccga
     4381 gatcgctgag gtgatgtatt ttgatgcagc tctgtcacaa accgaacagg acaaggtgtt
     4441 ctgctacctg aataccaagt ataacctggt atccgcgcgc cgggtgtgcg actcctaacc
     4501 cggcagttcc agcaactcca ggaaccgcgc ggtttttgat tctatttctt tctgcagttc
     4561 gtcaatcgaa cggcctaatt cactgatctt ctcgaaattg ccacccgcct gcatttctgc
     4621 attgagtgat tcgagccgtg cttcgagcat cggtatctct ttttcgagct ggtcgcgctc
     4681 tttcttttgc gcaaaggtaa tctgtttcgg gcgctctttc ggcttgcgct ctttgcggag
     4741 ctcttctttc tcttgttgaa tgacctgccg acttttggct tctttcgcgg cgaggtaatc
     4801 cgagtaattg ccgaggaaat cggtaatgac cccttcttca gtgtcacgga aatctttgtc
     4861 caggacaaag aggtgctgcg tcagcttgtc catgaagtag cggtcgtgcg agaccaccag
     4921 caggcagccg ggaaaatttt gcaggtattc ttcgagcacg gcaatggtag cgatgtcgag
     4981 atcgttggtc ggctcatcga gtataagaaa gttcggattc ttcatcagaa tcgtcagcag
     5041 gtacagccgg cgcttttcgc caccgctcaa ggtcgccgcc tgctgccggt gcgtcgccgg
     5101 cggaaacagg aactgctcga gcagctgcgc cgcagtgatc gacttgcctt tgggcaacgg
     5161 aatcgcttcg gcgacatcgc gcaccacgtc gagaatagtc ataccggccc tgaactcagg
     5221 cccctgctgg cggtaataac cgaaaaccgt ggtagctccc acttcaactt tgcctgagtc
     5281 tgcctgctca tgcccggtga taaggttgag gaaagtcgac ttgccaaccc cgttgcggcc
     5341 gatcacgccg atgcggtcgc ccttgagaaa atggtaggtg aaatcgctga cgatttggcg
     5401 ttcaccgaat tttttcgaga ggttgtcgat gtcgagaatc ttaccgccga cgcgcgaaat
     5461 ttcggtttcg agcagcgcga ggctctcttt cttgcgcgcg gtggcttcga ccttcaggtc
     5521 ttcaaacgcg ttgacgcgga acttggcctt ggtcgtacgc gccttcggct gtttgcgtat
     5581 ccattcgagt tcgcggcgca tcttgttctg cgccttttcg agcgatgcct gctctgcttc
     5641 ttcgcgctcg gcttttcttg caagataatt ggaataattt ccctggtagg caaataactg
     5701 gccgtcatcg agttcgagaa tctcgtcgca gatcgtttcg agaaagtagc ggtcgtgcgt
     5761 caccagcagc aacgtgatct ggtcctgctg cagataactc tcgagccact cgatcatatc
     5821 gaggtcgagg tggttagtcg gctcatcgag caccaaaaca tcggcttctt caatcagggc
     5881 atgagccagc gccacgcgtt tgcgctcccc gcccgaaaga tcggcaacct tcgcccccag
     5941 gttggtgata ccaagcctcg tgagaatgcc cttgatctta cgctcgtaat cccacgcgcc
     6001 ggcggcttca acgtcggcga tggccgcatc gatgccgcgc ccggtcgtca tggcttcttc
     6061 gtaacgcgcc agcaaccgca tgaccggctt cttgctgtcg aagatcgttt cgagaatggt
     6121 ctggctctca tcgagtaagt cggattgatc aaggtaagcg agggtgatgc cgttcttgag
     6181 caccacacgc cctgcatcgg cagattcgcg gccgacgagt atctgcatca gggtcgactt
     6241 cccggccccg ttcttggcaa tcaggccctt cttgtcgccg gcgttgagac caaacgagat
     6301 ttctttaaac aacggcttca cgccaaaggt tttactgagt ttttcaacag agaggtaatt
     6361 cagagactgc gcatactaga ctctgcacgc ggcgtcaatg ccgagcgaca atcgcgaaat
     6421 cgtattatta ttcttacgat atatagatcg tcttatcttt tttgattgtc tgcctgctcg
     6481 tgtggcgcag ggtggtcgtg ttatagtggg tggagtttat tctctaaccc aaggggtata
     6541 cgtgcaagct gtcatcaccg aattcaaatg ggagaaaaaa tactctaccg gcattctcga
     6601 gatcgatgcg cagcaccagt acctgtttgc cctcacgaac cgcctgctgc gcaatgtcgc
     6661 tgaaaaggtc agcctggcga caattggcgt cactattgcc cagcttgaag actacgtcga
     6721 tgaacacttc aagcgcgaag aagcgatgct ccgccgggcg aaatatgaat cactggataa
     6781 gcacatcgag caacaccaga aattacgttc gcagctgggt caatacggct ctcgcctgca
     6841 agaccagagt ctttcggtca cagattttgc gcacttcatg caactctggc tttccaaaca
     6901 catactgcac gaagatatga aatacctgcc ggctgtta
//
LOCUS       DAOQJF010000169         6853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6164_length_6853_cov_5.292292, whole genome shotgun sequence.
ACCESSION   DAOQJF010000169 DAOQJF010000000
VERSION     DAOQJF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6164_length_6853_cov_5.292292"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1931)
                     /locus_tag="PLF85_09840"
     CDS             complement(<1..1931)
                     /locus_tag="PLF85_09840"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE20011.1"
                     /translation="MSNVVRILFRLIAYFSSYGIAVATSAAISSILAISFGLYYARAT
                     NFPISELMLWTIAGGAAVAWIGHSIQFGILNKVGFPGFTRPVLAVNRFITATPDLQIR
                     PDLKESDYHELLRALTVIPLTNAFTAVLSVQFIFVGILIAAEMSVKFEPYHYVQGLVM
                     NIMFSFIHGGFCLVIGEVATGSMRAECKRIMSEKSIPFTDRAVTTVRLKLAFFILLFV
                     ITIYVAATLVYYNRDNLSAILVFTFMALVAAILMAYMIFYLIYSALKDIEGAMDDLKS
                     GGSGLIFTKSIDSEFVKVATGISDAARTIKDYQANLEQKIEERTRELQESLGNVRALK
                     GQQDGDYFLTSLLLKPLNGNYANNRQIKVEIFVRQKKSFEFKTKTNEIGGDICLAHSV
                     RLRGRDYTLFLNGDAMGKSMQGAGGSLVLGAVMQSIVERTKLSPKEQVLYPERWLKAV
                     FLELQKTFESFDGSMLISVVLGLADEKSGFIYFINAEHPWSAILRDNKAFFVEDSLTL
                     RKLGTLGVNMGIKVQTCELRPGDTFVVGSDGRDDLMLGTDESGARIINEDETLFLRLL
                     EQSGGGLDRLYESCKQTGEITDDFSLVSLRYQPEEETSEMRGGIRQKISEARRLLRER
                     NYAAATELLTGAYEISQDDV"
     gene            1958..2389
                     /locus_tag="PLF85_09845"
     CDS             1958..2389
                     /locus_tag="PLF85_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20012.1"
                     /translation="MVKAKMRRFLILDKNIFYLPPAMRISSMPTRVQASQYGQFFKRF
                     GLLSCGIVFLTGLQFFDPPAAESSRSQLQNSDSLHVAPMSDAVLEEAREGEYRLALGV
                     VGTAGFTGILLLISSSLLFRQLPVRQSFVNALHSSRAPPVI"
     gene            2486..5071
                     /locus_tag="PLF85_09850"
     CDS             2486..5071
                     /locus_tag="PLF85_09850"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNE20013.1"
                     /translation="MAKTNKQIRGKKGAQHKPAPDDHTVGAILAHLRHFLPSQGPIKD
                     FIHHNTLHMFQEQGMGFHEAVRSASELYGSVEYLPLSAYRSMHATGKITEAALAQVLS
                     AAGDTAGVLREQMLTGRYPDVLKRPGFRTSGYLKSITVAAGIMLEEQVHPTVFRLVAN
                     YLDQGIAAISMVHHAQDFWSALRLQIRAARPFGLSQNTSAWILRYEPEDLIGYLLDKI
                     LPASADRQTFLLELLMAARGWSGLIAQVEENPAYLNYPRQITLAQYVALYIAILSDLA
                     GQAGYKREELVAENRHSRYLATPVPAETEAERVARFWHEAMETSYYLETLAAIAENAP
                     VKRSRSLAEGKAEFQALFCIDDRECSVRRYLEELSDTTETFGTPGFFGIDAVYQGPFD
                     AISIKQCPVPVKPKHRIRGVAVSKRKVPLSRLEMNLWHRHANGLIAGWFVSLFFGVIS
                     LLRLVFSVHRPTRTFAMASSFAAHEDHTRLQYERAEGEAAKDGFFEGYTVNEMAERVG
                     RVLRQIGLTHSFAPIVAVVGHGSSSTNNPYFAAYDCGACSGRPGLVNARAFALMANRA
                     DVRALLARAGIRIPEKTRFVGAIHDTARDEIALLDAELIGDEYSADLTKLRQLFTQAL
                     ELNALERTRRFAILNFPESERAAVKEARLRTEMLFEPRPEYNHATNALAVVGRRSFTE
                     NIFLDRRAFFNSYDPAADRDGSILNGILTPLVPVCGGINLEYLFSRVDNSVFGAGSKL
                     PHNVFSLVGVGNGSEGDLRTGLPEQMIEIHDPVRLLIVIEQRREIVERVLGQNEAVRQ
                     WVDLEWVHLVVFDLKTKKFYRRFAGEFKELNLGTLKPPVFADSRAAYYHRRDNITPAI
                     LHRKH"
     gene            5073..>6853
                     /locus_tag="PLF85_09855"
     CDS             5073..>6853
                     /locus_tag="PLF85_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE20014.1"
                     /translation="MILRIVVESIVLYPLVIALLIAVLPWKESWLSRAVIIANVLNGL
                     LTAALLVLFIASGFHPVEISGGHLVQAGNYHFNFLLLVDKYSVSFQVLIGILTGLVLM
                     FSRRYMHREKGYKRFFASLFLFVAGISLVSLAATIDVLFAGWEIVGISSFLLIGFYRI
                     RLQPGRNSLRAYTIYRVCDVGLFMGAWLFHKLGSEYFFLQHADRALDAEFVQLNLWFF
                     TAIGLLTVLAAMGKSAQYPFSFWVPRAMEGPTPSSAIFYGALSIHAGVYLLIRMWPLW
                     RAVEWIPWLVGAVGLLTSVTATISLKTQSNIKGQVGYASVAQVGLMFIELAAGLREIA
                     MLHFLGNAILRCYQLLASPSIMVLYLRQQAEGAVLSRGFSGYAMSFLSEAWRRRFFAF
                     SFSEGYAEALMVFIWRKLKQLAGVVEQAFSPFVFINVVLTLLLLESFGRPFFAEGLIN
                     TICAVVFGFSSIFVALSALGQGVQPARVLWLTGLSAGFKGLSVVYLSPENAIHSLLYF
                     AGISTGFLIARAGLRFIPAAVQVTEYAGAAELYPRATNAYFIGILWMSGFTLSPTFFG
                     DDILLHTGAEKFAYETFFMAGAFVLNAV"
BASE COUNT         1438 a         1777 c         1879 g         1759 t
ORIGIN      
        1 acgtcgtcct gcgaaatttc gtacgcgcct gtcaatagct ccgttgcagc ggcataattt
       61 ctctcgcgca gcagccggcg ggcttcacta atcttttgcc tgatgccgcc gcgcatttct
      121 gacgtttctt cttccggctg gtagcgcagt gatacgagcg agaagtcgtc cgtaatttcg
      181 cctgtttgtt tgcaggattc gtaaagccgg tcaagtccgc cgccgctctg ctcaagcagg
      241 cggaggaaca gagtttcatc ttcgttgatg atgcgtgccc ccgattcatc ggtgccgagc
      301 atcaggtcgt cgcgaccatc ggagccaacg acaaaagtat caccggggcg aagctcgcac
      361 gtctgcactt tgattcccat gttgacgccc agcgtaccga gcttgcgcag cgtcaggctg
      421 tcttcaacga agaacgcctt attgtcgcgc aggatggccg accatgggtg ttctgcgttg
      481 ataaaataga taaatccgct tttttcatcg gcgaggccaa ggacgacaga aatcagcatc
      541 gagccgtcaa aactttcaaa agttttctgc agctcgagga atactgcctt cagccagcgt
      601 tcgggataaa gcacctgctc cttgggagaa agtttggtgc gttcaacgat ggattgcatc
      661 acggcaccca gaaccaaaga acccccggct ccctgcatgg atttgcccat cgcgtcgccg
      721 tttaagaaga gagtgtagtc gcgaccacgc aggcgcacgc tatgcgccag gcagatatcg
      781 ccgccaattt cgttggtctt cgttttgaat tcaaaacttt tcttctgcct gacgaagatc
      841 tctaccttaa tctggcggtt gttggcgtaa ttaccgttga gcggtttgag caacagagaa
      901 gtgagaaagt aatcaccgtc ctgctgtccc ttgagtgcgc gtacattgcc caggctttcc
      961 tgcagctcgc gcgtccgttc ttcaattttc tgttcaaggt ttgcctggta gtccttgatg
     1021 gtgcgggctg cgtcagagat gccggtcgcc accttgacga attcgctgtc gatcgatttg
     1081 gtgaagatta accccgagcc gccgctcttc agatcgtcca tagctccttc gatgtccttg
     1141 agtgcgctgt agatcaggta aaagatcatg taggccatga gaattgctgc aacgagggcc
     1201 atgaaggtaa acacgagaat cgccgaaaga ttgtcgcggt tgtagtagac gagggttgct
     1261 gccacataga ttgtaatgac gaacaggaga ataaagaaag cgagtttgag gcgtacggtc
     1321 gtgacagcgc ggtcggtgaa cggtatgctc ttctcgctca tgatgcgctt gcattcggct
     1381 cgcatcgagc cggtggcgac ttcgccaatc acgagacaga atccaccatg gataaaagaa
     1441 aacatgatgt tcatgacaag accctgcaca tagtggtagg gttcaaattt tactgacatc
     1501 tctgctgcta tgagaatacc gacaaaaata aactgaaccg agagcaccgc tgtgaaagca
     1561 ttcgtcagcg gtattacggt caatgcgcgc agcagttcgt ggtaatcaga ttctttaaga
     1621 tctggtcgta tctgtaggtc tggggttgcc gtgatgaatc gattcacggc aagcaccggc
     1681 cgagtaaaac cgggaaatcc tactttgttc agaatgccga actggatgga gtggccaatc
     1741 cacgcaactg ccgcaccgcc tgcgatggtc cagagcatga gttcgctgat cgggaagttc
     1801 gtggcccttg cgtaatagag accgaaggag attgcgagta tcgagctgat cgcggctgag
     1861 gtggcaaccg caataccgta tgaggaaaag taggcaatta accggaacag gatccggacc
     1921 acgttagaca tggcacatga gttgcagtga gaattgcgtg gtaaaggcga aaatgcggcg
     1981 ttttctaatt ttggataaaa acatatttta tttgccgcct gccatgcgga ttagtagtat
     2041 gcccactaga gtgcaggcct cgcagtacgg gcaattcttc aagcgctttg gcctgttgag
     2101 ctgtggcatc gtctttctta cggggttgca gttttttgat cctccggcgg cagaatcctc
     2161 gcgttcacag ctgcaaaaca gcgatagcct gcatgttgcc cctatgtccg acgcggtact
     2221 tgaagaggcg cgtgaaggtg aataccggct tgcactcggc gttgtcggca cagcaggctt
     2281 taccggcatt ctgctcctta tttcctcttc tttacttttt cggcagttgc cggtgagaca
     2341 aagtttcgtc aatgcactgc attcttcccg ggctccccct gtaatctgat agcagcgaac
     2401 cacggattgc agccccgggc tgcctgaact ggacttcact gctgccgctt gctcgcggca
     2461 taaagattga ttttagggga gttgaatggc aaaaacgaat aagcaaatcc ggggtaagaa
     2521 aggggcgcag cacaaaccag ccccggacga tcataccgtg ggagcaatac tcgctcacct
     2581 gcggcacttt ctgccttcgc aggggccgat taaagatttt atccatcaca ataccctgca
     2641 catgtttcag gaacagggca tgggatttca cgaagcagta cgttctgcct cagaactgta
     2701 tggttctgtc gaatatctcc ctttgtctgc ctatcggtcg atgcatgcca cgggaaaaat
     2761 cactgaggcc gccctggcgc aggtgctttc tgccgccggt gataccgcag gcgttctgcg
     2821 cgagcagatg cttaccggtc ggtatcccga tgtactgaag cgtccgggtt ttcgtacttc
     2881 tggttacctc aaatcgatca ccgtggccgc tggcatcatg ctggaagagc aggtgcatcc
     2941 gacggtattt cgactggtcg caaattacct cgatcagggt atcgccgcaa tttccatggt
     3001 gcaccatgcg caggatttct ggagtgcatt gcgtttgcag atcagggctg cgcgaccctt
     3061 tggtttgtca cagaatacca gcgcttggat tctgcggtat gagccagagg accttatagg
     3121 ttatttgctc gataaaatac tgccggcatc tgctgacagg cagacttttc tactcgaact
     3181 gctcatggca gcccgcggct ggtcaggcct cattgcacag gtcgaagaaa atcccgcata
     3241 ccttaactac ccgcgccaga taaccctcgc acagtacgtg gcgctctaca tcgctatttt
     3301 gagtgatctg gcggggcaag cagggtacaa gcgcgaagag ctggttgcgg agaacaggca
     3361 cagtcgttat ctcgcaacgc ccgtgccggc cgagactgag gcagagcgcg ttgcgcgatt
     3421 ctggcatgag gcgatggaaa ccagctatta cctcgaaacg ctggccgcta ttgctgaaaa
     3481 tgcaccggtg aaacggtcgc gttcgttagc tgaaggcaaa gccgagttcc aggcattgtt
     3541 ctgcatagac gaccgcgaat gttcggtcag gcgctacctt gaagaattat ccgacacaac
     3601 agaaacattt ggtactcccg gcttctttgg catcgatgca gtctaccagg ggccttttga
     3661 tgcaatttcc atcaaacagt gtccggtacc agtaaaaccc aaacaccgca ttcgcggcgt
     3721 tgcggtgagt aagcgcaaag tacccctgag ccgtctcgag atgaatctct ggcaccgtca
     3781 cgcgaatgga ctcattgcgg gttggtttgt ttcgctgttt ttcggagtta tttctcttct
     3841 gcgcctcgtc ttctcggtgc accgcccgac gcgtactttt gcgatggcca gttcttttgc
     3901 cgcgcatgaa gaccatacac ggctgcaata tgagcgcgcc gaaggcgaag cggcaaaaga
     3961 cgggttcttt gagggctaca cagtcaatga aatggcagaa cgcgtcggtc gcgtcctgcg
     4021 tcaaataggc ctcacgcaca gttttgcccc gatcgttgca gtggtaggcc atggctcgag
     4081 cagcaccaat aatccgtatt ttgccgccta tgactgcggt gcctgttcgg gcaggcctgg
     4141 actcgtcaat gcccgggctt ttgcgctgat ggcgaaccgc gctgatgtgc gtgcgctcct
     4201 cgcgcgggcc ggcatccgca ttcctgaaaa aacgcggttc gtaggtgcga ttcacgatac
     4261 agcccgcgat gaaattgcgc tgctcgatgc cgagctcata ggcgatgaat attctgccga
     4321 tctcacaaaa ctcaggcagc ttttcacgca ggcgctcgag ctgaatgcac ttgagcggac
     4381 gcgcaggttc gcgattctca attttcccga atcggagcgt gccgcggtca aagaagcacg
     4441 tcttcggacg gaaatgctgt ttgagccccg tcctgaatac aaccacgcga ccaatgctct
     4501 tgcagttgtg ggccggcggt cttttacaga gaatattttt ctggaccgac gagcattttt
     4561 taactcgtat gatccggctg cagaccgcga tggcagtatt ctgaatggaa ttctgacccc
     4621 gctggttccc gtatgcggag gtattaatct ggagtacctt ttctcgcgcg tcgacaactc
     4681 ggtctttggt gcaggctcaa aattgcctca caacgtcttc tcactggtgg gtgtcggtaa
     4741 tggttctgag ggcgatctgc ggactggact gcccgagcag atgatcgaga tacatgaccc
     4801 ggtgcgtctg ctcatcgtga ttgagcagcg ccgtgagatt gtagaacgtg tattgggaca
     4861 aaatgaagcc gtacggcaat gggtagatct cgagtgggtt cacctggttg tttttgatct
     4921 gaagacgaaa aagttctacc ggaggttcgc cggggaattc aaagaattaa acctcggcac
     4981 ccttaagccg cctgtctttg cagattcccg ggcggcatat taccatcgcc gtgacaatat
     5041 cacaccggcg attttacaca ggaaacactg aaatgatact tcgtattgtt gttgaatcga
     5101 tcgttctcta cccgctggtt atagccctgc tgatcgcagt gctaccgtgg aaagagagct
     5161 ggctgtcccg ggcggttatc attgccaatg tgctcaacgg tttgctgacc gcagcgctcc
     5221 tggtattgtt catcgcatct ggatttcatc ctgtggagat ttcaggtggg cacctggtgc
     5281 aggcgggtaa ctaccatttt aattttctgc tgctcgttga taagtactcc gtttccttcc
     5341 aggtactcat cggcatactg accggcctcg tgctgatgtt ctcgcgacgc tatatgcacc
     5401 gcgagaaggg ttacaaaaga ttctttgcgt cgcttttcct gtttgtagcc ggtatttccc
     5461 tggtttcgct ggcggcgacc atcgacgtgc tctttgccgg ttgggaaatc gtcggaattt
     5521 cctctttcct gctgatcggt ttctatcgca tccggttgca gccgggacgg aacagcctgc
     5581 gcgcctacac catttaccgt gtctgtgacg tgggattatt catgggggcc tggctctttc
     5641 ataagctagg cagcgaatac ttcttcctgc agcatgccga ccgggctctc gacgcggaat
     5701 ttgtccagtt aaacctctgg ttttttacgg cgattggttt gctaacggta ctcgccgcca
     5761 tggggaaatc tgctcaatat cccttttcgt tctgggtacc gcgcgccatg gaaggtccca
     5821 caccgtcgag tgccatcttt tatggtgcgc tctctattca tgctggagtt tacctgctca
     5881 ttcgcatgtg gcccctgtgg cgcgctgtgg aatggatacc ctggctggtc ggtgcggttg
     5941 ggctgctgac ttccgttacg gcaaccatct ctctcaaaac gcaatcgaac attaaaggcc
     6001 aggtcggtta tgcctcggtg gcacaggtgg gattaatgtt catcgagctg gcagctggcc
     6061 tgcgtgaaat tgcgatgctg cattttctcg gtaacgcgat tctacgctgc taccagcttc
     6121 tcgcctcgcc ttcgatcatg gtgctgtacc tgcggcagca ggccgagggc gcagtgctga
     6181 gccggggatt ttccggttac gccatgagct ttcttagcga agcatggcgg cgtcgcttct
     6241 ttgctttctc tttctctgaa ggttatgcag aagcactcat ggtctttatc tggcgcaagc
     6301 tgaagcagct ggcgggtgtc gttgaacagg cattttctcc gtttgttttc atcaatgtgg
     6361 tgcttaccct gctgctgctc gaatcctttg gacgaccatt ttttgccgaa ggcctgatca
     6421 ataccatctg cgcagtggtg tttggctttt cctcaatttt tgtagctctc tctgcgctgg
     6481 ggcagggggt acaaccggcg cgggtactgt ggctgacggg cctatctgca ggttttaagg
     6541 ggttgtcggt tgtttacctt agcccggaga acgcaatcca tagcctgctg tactttgcgg
     6601 gtatctcaac gggctttcta atcgcacgtg ccggattgcg ctttatacct gcagctgttc
     6661 aggtaaccga gtatgccggt gcagcggagc tttacccgcg ggcgacaaat gcttacttta
     6721 tcggaattct ttggatgtca ggttttaccc tgagcccgac cttttttggt gacgatattc
     6781 tgctgcatac gggagcagaa aaatttgctt atgagacatt tttcatggcg ggtgcatttg
     6841 tgctcaatgc ggt
//
LOCUS       DAOQJF010000170         6852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6167_length_6852_cov_6.383257, whole genome shotgun sequence.
ACCESSION   DAOQJF010000170 DAOQJF010000000
VERSION     DAOQJF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6167_length_6852_cov_6.383257"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1466
                     /locus_tag="PLF85_09860"
     CDS             195..1466
                     /locus_tag="PLF85_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE20015.1"
                     /translation="MNFYRNYIYPRFSDRRLEHRFILDNNEKAARQARWGFAFGTFGY
                     AMLGAIAFFIDEAMFARTKWLVYGLIMPTHSLAAFGVTYLARFFYLQWVLVFANIFSN
                     ACVIGILYFAPEDFYIKYGYGLVICILIYTFAMLRMRTFFAIVGSSLVIMQYVAFLLL
                     VRPMLLNDAIYGLALISFVNTAGLFSVFFFEQNSRTEFMQSRTIEEQSLLLNEEKKKS
                     EALLLNVLPESVANRLLSGERTIADYFDSVSVLFADIEGFTSLSRRLNPHDLVQMLNR
                     IFSHFDALAGVLGLEKIKTIGDAYMVGAGLPAPVSDHAQRCFDMALGMLRILEDENKE
                     LPEPLKLRIGIASGPVVAGVIGQNKFIYDLWGETVNTASRMESSGVAGEIHITASTKE
                     ALGDAAEYIDRGEIEIKGIGKMTTYLARPLT"
     gene            1506..3488
                     /gene="thrS"
                     /locus_tag="PLF85_09865"
     CDS             1506..3488
                     /gene="thrS"
                     /locus_tag="PLF85_09865"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNE20016.1"
                     /translation="MLVVRVTIGDQNGEFPPETTFGEVVEKLAPAFKKSALGIEVDKV
                     SYDLSRPLSVIGSQAIAARPLTFDDEAGKEIFWHSSAHVLAQALKRVYPHIQLEDGPV
                     VKNGPGFFFYDVLLDDKINEEAFEKIEAEIQKVIRENHAVKRTVLERKDAVAKFKAMG
                     EHLKVGIIEKLPEGDEIRVYEQGEFADLCRGPHVPSTGKLGVFKLTTVAGAYIGGKSE
                     NQMLQRIYAVSFPDKKLLAEHFKRVEEAKKRDHRKLGTELELYTTSEDIGPGLILWLP
                     RGNIIKDELENWAKETEARNGYQRVTTPVLTKENLFFTSEHLPHYMDSMFPPMQMDNE
                     NYYIKPMNCPFHHTIFGHRPRSYRELPLRLAEYGTCHRYEDSGALMGLMRVRAMQMND
                     AHIYCTEEQAVEEFKAVIKLHEYYYKALNITDYHMVLSLRNPANKKYHGETEMWDKAE
                     ALTRQAMEESGVQYIVENDGAAFYGPKMDFQIKSSIGREFTSSTCQLDLFMPMKFDLK
                     YVDKDGTLKRPVCIHRSPLGTHERFIGFLIEHFAGAFPLWLAPEQVRILPVNEAHMEY
                     ASKLQQAFRDAGIRVHTEVPEESLGKRIRTAEKLKVPYMLVVGDKEASGNMINARNYF
                     TGEQREYTLQDFMATVQEEIRSKRITEIAKPAPAAK"
     gene            3548..4174
                     /locus_tag="PLF85_09870"
     CDS             3548..4174
                     /locus_tag="PLF85_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002097449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20017.1"
                     /translation="MKKTENRRFTEAELNRFYELLDESLTPYDCGKLCAPSNGGEPLC
                     CQVENAVPMLYREEFEYLQKRTELWDRWKPKTPIDKKMKKEDETRILIFCECKGVQHC
                     ERENRSVGCRTFPLEPYFDEKGEFVGLIFMKEFRNKCPLISRLKDIKQSVIDKHYEFW
                     TLIMEIKKDEFDLYKSTSRGWRISATKRGTKLPILYPSHKKTRKAVAG"
     gene            4239..6173
                     /locus_tag="PLF85_09875"
     CDS             4239..6173
                     /locus_tag="PLF85_09875"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNE20018.1"
                     /translation="MAELTLSERISHAPDSCGVYQWFEHAGDRTPLYVGKARNLRSRL
                     RQYLNSDDLKTTFLMRRASHIEWIATDNETEALLLENNLIKKHRPVYNVRLKDDKQYP
                     YICISMSEPFPRIYLTRRRLPGNLYFGPFSKAQVAREKIQLTLDMFPLRRRPLKLPLA
                     KPAKPCLNYHLKKCLAPCAEKISQHDYAAIVQQAIDFISGSDDEIRQQVEKKMHRLAA
                     EMKFEEAARLRDILAYFNERNITPQVEMRDALPDFDIVGIYSAPLEQLKADLSLRDED
                     LSARKSAPGADLRMAQICLLKFRDGRLIAKENFSVTETDMNSATLSDAEFAETFFRDY
                     YLGMIDLPPYIVVPDGLDLASWSEALKTGLRVLGLAEFSQAVKDANKEVNPAALMQMA
                     NNNARLVMRERLLSENLRNQRVGLKQLQKFIGLKNPPQVIECYDISNIQGTDPVASGV
                     MLRDGMPYKSGYRKYRIKTIEGANDPAMMHEALSRRLARVKSGEVQPADLIVIDGGST
                     QLNAALQARSEAGLSIPMIGLAKKQEEIYTEDGRILRFDKESPGMQIIRRARDEAHRF
                     AVSYHRNLRMKRNLRSVFDGVEGIGEKKRAAILLALRKLDLGEIDSTALAKKIRKESG
                     VTAEQSEALAQKIFAGLKGQ"
     gene            complement(6126..6518)
                     /locus_tag="PLF85_09880"
     CDS             complement(6126..6518)
                     /locus_tag="PLF85_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNE20019.1"
                     /translation="MPTYSDELLRLAEVVPTPIPEGAAVVERANRLCGDRVVVSPEIQ
                     GNVVVALRWHSEGCAILKAGAAYLARGIRGKDTTTALAWITEFKDSFNSEAAHLPGAM
                     AAVYGLPARYKCALLPFEACEDFLRQRL"
     gene            complement(6518..>6852)
                     /locus_tag="PLF85_09885"
     CDS             complement(6518..>6852)
                     /locus_tag="PLF85_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE20020.1"
                     /translation="HRLQSAIGDDAELKAKIALLFISVDAANDKPADLSRYLASFPYA
                     RGFTGTAEEIRRVEKMFGAYSKEEKGTISHSLYLYALNKQGKVIYLIRYDDSVEKIRD
                     VLQQAAKL"
BASE COUNT         1670 a         1828 c         1857 g         1497 t
ORIGIN      
        1 gcattgtcag aacaaatcct gaggggttcc aaatagtctc acgcggcaaa aggtacgaat
       61 agcagcagat tggtcgtgta ggccagggct tcgacagcat aatctccgag accgtaagac
      121 cccctccgcg cgctccgcgg ctccgcgtga tctctttgta ttgccaactc cgctcaattt
      181 ctgagtctca gccagtgaac ttttaccgca actacatcta cccccgtttc agcgatcgca
      241 ggctggagca ccggtttatt ctcgataaca acgagaaggc agcaaggcag gcgcgctggg
      301 ggtttgcgtt cggtaccttc ggctacgcga tgctcggagc cattgcgttc tttatagatg
      361 aggctatgtt cgcgcggacg aagtggctgg tgtatgggct catcatgcct acgcattcgc
      421 tggcggcgtt tggagttacc taccttgccc gcttctttta tctgcagtgg gtgctggtat
      481 ttgccaatat cttcagcaat gcctgtgtca ttggaatttt atactttgca ccagaggatt
      541 tttatattaa atacggttat ggtttggtta tctgcatact gatctacact ttcgcgatgc
      601 tgcgcatgcg taccttcttt gcgattgtcg gttcttcgct cgtgattatg caatacgttg
      661 cctttttgtt actggtgcgc cccatgctgc tgaacgatgc tatctacggc cttgctctta
      721 tttcgtttgt taatacggca ggactgtttt ctgttttctt ctttgaacag aactctcgca
      781 ccgagtttat gcagagccgt acgattgaag aacagagcct gctgctgaat gaagagaaga
      841 aaaaatcaga ggcgctgctg ctgaacgtct tgcccgagag tgttgccaac cgcctgcttt
      901 cgggtgagcg cacaattgcc gactactttg acagcgtttc ggtgctgttt gccgatattg
      961 aaggtttcac cagtctctcg cggcgcctga acccgcatga cctggtgcag atgttgaacc
     1021 gcatcttcag ccatttcgat gcgctcgccg gggttctggg tctcgagaaa atcaagacta
     1081 tcggtgacgc ctacatggtc ggggcaggtt tgccggcgcc agtcagcgac cacgcgcagc
     1141 gctgctttga catggctctc ggtatgctgc gtatcctgga agacgagaac aaagaattgc
     1201 cagaacccct gaagctccgc atcggtattg cgtcggggcc ggtggttgcg ggagttattg
     1261 gacaaaacaa attcatctac gatctgtggg gtgaaaccgt caacactgcc agccgcatgg
     1321 agtcatcagg tgtagcgggt gaaatacata taacggcatc gacgaaagag gcgctcggcg
     1381 atgcagcaga atatatcgac cggggtgaaa ttgagattaa gggcataggc aagatgacca
     1441 cctacctcgc gagaccgctc acctaattca ctttatcgcc tgtcatagaa tcgtgttttg
     1501 ccaccatgtt ggtagtacgc gtaacgatag gtgaccagaa cggagagttc cctcccgaga
     1561 ccacatttgg cgaggtcgtc gaaaaactcg ccccggcctt taaaaaaagc gcgctcggta
     1621 tcgaggtgga taaggtctct tacgacctga gccgccccct gtcggtgatt ggctcgcagg
     1681 caatcgccgc acggccgctg acttttgacg atgaggccgg aaaagaaatc ttctggcatt
     1741 cgagcgcgca cgtgctcgca caggcgctga agcgcgttta tcctcacata cagctcgaag
     1801 acggcccggt ggtaaagaat ggccccggtt tctttttcta cgatgtgctg ctcgacgaca
     1861 agatcaacga agaagcattt gagaaaatcg aagctgagat tcagaaagtc attcgtgaaa
     1921 accacgcagt gaagcgcacg gtgcttgagc gcaaagacgc agtcgcgaag ttcaaagcga
     1981 tgggcgaaca tcttaaggta ggaatcattg aaaaactgcc agagggcgac gaaatacgcg
     2041 tctacgagca gggcgaattt gccgacctct gccgcgggcc acacgtgcct tcgaccggca
     2101 aactgggagt ttttaagctc accactgtcg ccggcgccta tatcggtggc aaatcagaaa
     2161 accagatgct gcagcgcatt tatgcagtaa gctttcccga caagaaactt ctggctgagc
     2221 acttcaaacg cgtagaagaa gcgaaaaagc gcgatcaccg caagctcggt acagaactcg
     2281 aactctatac cacgagcgaa gatattgggc cggggctcat tctctggcta ccccgcggca
     2341 acatcataaa ggacgaactc gaaaattggg cgaaagagac cgaggcgaga aacggctacc
     2401 agcgcgtcac gaccccggtg ctgacaaagg aaaatctttt tttcaccagc gagcacctgc
     2461 cgcactacat ggatagcatg tttccaccga tgcagatgga taacgaaaat tattacatca
     2521 agccgatgaa ctgcccgttt caccacacga ttttcggcca ccgtccgcgc agctaccgcg
     2581 agttgcctct gcgtctcgca gagtatggca cctgccatcg ttacgaagat tcaggggcgc
     2641 tcatgggtct tatgcgtgtg cgcgctatgc agatgaacga tgcacacatt tattgcaccg
     2701 aagagcaggc agttgaagag ttcaaagccg tcatcaagct gcacgaatat tattacaaag
     2761 cgctcaatat caccgactac cacatggtgc tttcgctgcg aaatccagcg aacaaaaagt
     2821 accacggcga aaccgaaatg tgggacaaag ccgaagcact gacgcggcag gcgatggaag
     2881 agtctggcgt acagtacatc gtcgaaaacg acggtgctgc attttatggc cccaagatgg
     2941 actttcagat taagagttca atcgggcgtg agttcacttc ttcaacgtgc cagctcgacc
     3001 tgttcatgcc gatgaaattt gatctgaagt atgtcgacaa agacggcacg ctgaaacgcc
     3061 cggtgtgcat ccaccgatcg ccgctgggta cgcacgagcg cttcatcggc ttccttatcg
     3121 aacattttgc cggcgctttt ccgctctggc tggcccctga gcaggtgcgc attctgcccg
     3181 tcaacgaagc acacatggag tatgcctcga agttgcagca agctttccgt gatgcgggta
     3241 ttcgcgtgca tactgaagta cccgaagaaa gcctcggcaa gcgcatccgt accgcggaga
     3301 aactcaaggt accgtacatg ctcgtggtcg gtgacaaaga agcgtcgggc aatatgatca
     3361 acgcgcgcaa ctatttcacc ggtgaacaac gggaatatac gctgcaggat tttatggcaa
     3421 cagtacagga agaaatccgc agcaagcgca tcaccgagat tgccaagccg gcgccggcag
     3481 ctaaataacc attgcgaccg ggtggcagtc tcgctgctgc ccggttatat gcccaaatac
     3541 caaaaccatg aaaaaaacag aaaacagaag atttactgag gcggagctga atcgcttcta
     3601 cgagctgctc gatgaaagcc tgacccctta tgactgcggc aaactctgcg ctccctcgaa
     3661 cgggggtgag ccgctgtgct gccaggtcga gaatgccgtg ccgatgctgt accgcgaaga
     3721 atttgagtac ctgcaaaaac gtaccgaact atgggaccgc tggaaaccca agaccccgat
     3781 cgacaagaag atgaaaaaag aagatgagac gcgtattctc atcttctgtg agtgcaaagg
     3841 agtacagcac tgtgaacgcg aaaaccgttc cgtcggctgc cgaacctttc ctcttgaacc
     3901 ctatttcgac gagaagggcg aatttgtcgg cctgatcttc atgaaagagt ttcgtaacaa
     3961 gtgtccgctt atcagccgcc tgaaagatat caagcagtcg gtgatagaca agcactacga
     4021 attctggacg ctgatcatgg agatcaagaa agacgagttt gacctgtaca aatcgacctc
     4081 gcgcggctgg cgtatttccg caaccaagcg cgggacaaaa ctgccgatcc tgtatccttc
     4141 gcataagaaa accaggaaag cggttgccgg ttaggtttag cgcaaactcc aattgccctt
     4201 tacgggctgc cgcaattcct gcctgaccca gttgtgccat ggctgaactg acgctctctg
     4261 aacgtatctc acacgcgcca gatagctgcg gcgtgtacca atggttcgag cacgcgggcg
     4321 atcgcacacc gctctacgtc ggtaaggccc gcaacctgcg tagccggctt cgccagtacc
     4381 tgaactccga cgacctgaag accactttcc tgatgcgccg ggccagccac atagagtgga
     4441 tcgcgaccga taacgaaacc gaagccctgc tgctcgaaaa caacctgatc aagaaacacc
     4501 ggccggtgta caatgtcaga ctcaaagacg acaagcaata cccctatatc tgtatctcga
     4561 tgagcgaacc gtttccgcgt atttatctca cgcggcggcg tttgccaggc aatctgtatt
     4621 tcggtccctt ttcgaaggcg caggtggcgc gcgagaagat acagctgacg ctcgatatgt
     4681 ttccgttgcg ccgcaggccg cttaagttgc ccctcgcaaa accggcaaaa ccctgcctta
     4741 actatcatct caaaaaatgc ctcgcgccgt gcgccgaaaa aatcagccag cacgattatg
     4801 cggctattgt gcagcaggcg atcgatttta tttccggcag cgatgacgag attcgccagc
     4861 aggtcgaaaa aaagatgcac cggttggcgg cagaaatgaa gttcgaagaa gccgcgcggc
     4921 tgcgtgatat ccttgcttac ttcaatgagc gcaacatcac cccgcaggtc gagatgcgcg
     4981 atgcgctgcc cgattttgac atcgtcggta tctattcggc tccgcttgaa cagttgaagg
     5041 ctgatctttc tctgcgcgat gaagaccttt ctgcacgcaa atcagcgccc ggcgctgatt
     5101 tgcgcatggc acagatctgc ctgctcaagt tccgcgacgg ccggttgatc gccaaagaga
     5161 acttttcggt aaccgaaacc gatatgaact ctgcgaccct cagcgatgca gagttcgccg
     5221 agacattttt ccgcgactat tacctcggca tgattgacct gccgccgtat attgtcgtac
     5281 ccgatgggct ggatctcgca agctggtcag aggcgctgaa gaccggattg cgcgtgctgg
     5341 ggctcgcgga attttcgcag gcggtgaaag acgccaacaa ggaagtaaac cctgctgcgc
     5401 tgatgcaaat ggcgaataac aatgcgcggc tggtcatgcg cgagcgcctg ctctccgaga
     5461 acctacgcaa ccagcgtgtg ggccttaagc agctgcagaa gttcattggc ctgaaaaatc
     5521 caccgcaggt tatcgagtgc tacgacatct caaatatcca gggtacagac ccggttgcga
     5581 gcggcgtgat gctgcgcgac ggcatgccgt acaagtcggg ttatcgcaag taccgtatca
     5641 aaacgattga aggtgcgaat gacccggcga tgatgcacga agcattgtcg cgcaggctcg
     5701 cgcgggtcaa gagcggcgaa gtgcagccgg cagatctcat tgtcatcgac ggtggcagta
     5761 cgcagttaaa tgccgcgctg caagcccgct cagaggctgg cctcagtatt ccgatgattg
     5821 gccttgccaa gaagcaggaa gaaatttata ctgaagacgg gcgcatcctg cgttttgata
     5881 aagaaagtcc cggcatgcag attatccgcc gcgcacgcga tgaagcgcac cgctttgctg
     5941 tttcctacca ccgcaacctg cgcatgaagc gtaacctgcg ttcggtcttt gatggcgtcg
     6001 aaggtattgg ggagaaaaaa cgcgcggcaa tattgctggc gctgcgcaag ctcgacctgg
     6061 gtgaaatcga ttctacagct ctggcgaaga aaatacgtaa agagtcagga gttaccgcgg
     6121 agcagtcaga ggcgctggcg caaaaaatct tcgcaggcct caaagggcag taacgcgcac
     6181 ttgtagcgcg ccggcagtcc gtagactgca gccattgcac ccggcaggtg agcggcttcg
     6241 ctgttgaacg aatctttgaa ttctgtaatc cacgcaaggg cggttgtcgt atctttgccc
     6301 ctgatgcccc gggcaaggta ggcggcgccg gcttttaaga tggcacaacc ctcagagtgc
     6361 cagcgcaacg cgacaacgac attgccctgg atttcaggcg agaccacgac gcgatcacca
     6421 cacagccggt tggcgcgttc aacaaccgca gcgccctcgg gtattggtgt aggaactacc
     6481 tcggcgagac gtagtagttc gtcagagtag gtcggcatca gagctttgcg gcctgttgca
     6541 gcacgtcgcg gattttttcg actgaatcat cgtatcgtat caggtaaatg actttgccct
     6601 gtttgttcag agcatagaga tagagcgagt gcgaaattgt gcccttctct tctttggagt
     6661 aagcgccgaa cattttttcc acgcggcgaa tttcttcggc agtaccagta aaaccccgcg
     6721 cgtacgggaa actggccaga taacgtgaga gatccgccgg tttgtcattt gctgcatcga
     6781 cgctgatgaa gagtagagca atttttgcct tgagttcggc atcgtcgcca atggcagact
     6841 gcaggcggtg ca
//
LOCUS       DAOQJF010000171         6844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6174_length_6844_cov_4.523347, whole genome shotgun sequence.
ACCESSION   DAOQJF010000171 DAOQJF010000000
VERSION     DAOQJF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6174_length_6844_cov_4.523347"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PLF85_09890"
     CDS             complement(<1..1014)
                     /locus_tag="PLF85_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE20021.1"
                     /translation="MLKFFSLWLALFATYHIFVLVFAGIGVLSAGSCLHAFLADCGWA
                     GLLGAVFAALRLRAWRWPVRLAYTVLILFLVVAFGFARLYQRPFSFAFVRLDTGTIWK
                     ENLVSGLYELGLAHLVLAVACAAILFWQSRSAVALGRRGWAVGFSSATLLVVLHWLSP
                     AEPQLIANPLSAAFSRRAEATAMVGVDLADNDLLPSLESAELTRALPQVNVQRIEGRN
                     VILYYLESTPFSVIGKRVQDKEVTPHLNRLSERSLFFTRHYANFPLSINAFYNSFCSA
                     YALPDGAWISLVLPDFKVPCLSEVLGKEGYRAAALHAGYLGYAKQKRFLERRGFSLMM
                     DAET"
     gene            1117..1806
                     /locus_tag="PLF85_09895"
     CDS             1117..1806
                     /locus_tag="PLF85_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20022.1"
                     /translation="MRYKWQNMKNPSWDYPEIYSTVTIHYDQIKVETNRALGQVRLEV
                     KTLRGEHYESIIRQGTILREKDLRTGALTDLTDILSVYRKDFSSLPDTPILALIGGNY
                     GIMTEFMGTAERIVVHDRHPFRRFMRLLRGIWPALKRWYGSTRANFGYYRHAARKADI
                     FDVALIGSSTYAAFTYNFDYLNAGFTLCIVAMLTGLIDWLIRKREPWLLKIYASVALG
                     TVAVYRGYFFQ"
     gene            complement(1800..2069)
                     /locus_tag="PLF85_09900"
     CDS             complement(1800..2069)
                     /locus_tag="PLF85_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HNE20023.1"
                     /translation="METEVLGFIAGMITTISFVPQVVRIYRIKSGRDISLWMMLLFAL
                     GVTLWLIYGLLLSSLPIILANAVTLVLVVAILVLKIYYARNQQRY"
     gene            complement(2078..3610)
                     /locus_tag="PLF85_09905"
     CDS             complement(2078..3610)
                     /locus_tag="PLF85_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNE20024.1"
                     /translation="MAALFAGVSVFGWWFLRRAGYKPRFVGPALDRGHKLREPYTATV
                     GTAQLTVPVAIIGGGISGMSAGWYLQKHGLKDFRLFELEDRAGGNAQWGENRNGRYPW
                     GAHYLPTPGTDAVYVRELLAEMGIYQDGRFSEEALVHETEERLFIYGNWQPGLVPLMG
                     ARESDRQQIELFFRTMSAFSAARGRDGRRAFTIPLALSSADPKYVQYDAIPAAAYLDQ
                     LGISSPRLLWYIDYVLRDEYGSNRHNTSAWALLHYFAARSEAHNLVWPEGNGFIAEYL
                     RKKLGNTIQPATALRHMEKTGSGYRLHFFNHATQSVEVCIARKVIFAAPKFVLPWVYP
                     GLRSDQRAAAKALVYSPWLTVNLLVNHFGENAPAWDNVMFGSKSLGYVVAEHQRAGKL
                     PHARTLTFFHAFDEDDTLQSRRRLLQMTEHEALQMALDDIGRAHPQVNEFVESACIYR
                     WAHAMVRPTPGFLARMRGGLLEKIDENFYGAHSDVSGMSNFEEAQYRGVMAARRVLGE
                     IS"
     gene            complement(3661..4680)
                     /locus_tag="PLF85_09910"
     CDS             complement(3661..4680)
                     /locus_tag="PLF85_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNE20025.1"
                     /translation="MAQRPGSLLRIRLLYHYIRLFTRPILVLLVLLVTGSAGYVMLEG
                     FTPFEGLYMTVISITTVGFSEVRPLSDAGRVFTICLILGGVMFYGLGINAVIQIILER
                     GFRGFMDAQILSEKLRNIHGHYIVCGGGRMALAICSELEKAGRDFLVIEKNNDSPVSR
                     LVSERKVAWAVLSGDALSEEVLREARIDRARGLFSVLPTDADNLFVVLSARGLNPTIW
                     IETRISHETTRAKMVQAGANRVLSPYHVAGIQMARSMLKPQVDDFIEIFRGDRNYEFE
                     VKIHRLTAGDEGVGKNVGACRYIEQGFSVVALQNPDGSMIYAPAPETTMRAGMSVFLL
                     GKGRT"
     gene            complement(4685..5530)
                     /locus_tag="PLF85_09915"
     CDS             complement(4685..5530)
                     /locus_tag="PLF85_09915"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNE20026.1"
                     /translation="MSQQGALPDFHVAFAAEALTKILRAEIQKTGLRKAVLGVSGGVD
                     SALSLALAVNALGKENVLAVMMPYKSSNPDSLGDARKLCNKFGVATEVVEITAMADAY
                     FSRDESITATRRGNVMARLRMIVLYDLSARDASLVVGTSNKTEILLGYSTLWGDMASA
                     VNPLGDLYKYQVFAIARQMGVIPEILDKAPSADLWSGQTDEAEMGFSYAMADLVLYHW
                     MERNYTVPALKEMVRGYGQDEAVVDQILKRVERNAYKRNMPVIAKLSPVTIGREFRTP
                     RDWGM"
     gene            complement(5577..>6844)
                     /locus_tag="PLF85_09920"
     CDS             complement(5577..>6844)
                     /locus_tag="PLF85_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE20027.1"
                     /translation="KKPAENPYATRDLFIAVKNRNLAGAQKALAERASINAADEEGKS
                     PLSYAVELNDREIAEFLRQNGGLDAVAGRRMLRAIAANNATEFVRAMDDSGDPNYTDK
                     FGNTPLLVAAAYGNAEILQTLLELKANVHSTDADGYTALFYAAQENHVRIIDQLIAAG
                     AGVNAQSRFGFSPLHIAAVRGQLQAVQRLVQAGADVNAVAKQGGTAAYWAALGGHDEV
                     ADFLLKSGSADFHNNLELAQAARTNNVALAQAALRATELRIDMQDIEGSSALFYAAAN
                     GSAEVARLLIARGANVNLLNHQGQSPLLVATLKGHLPVVELLVQAKANTEAKDAQGRS
                     AVMHAAWKGNAELVKILVNLRADVNRQDNEGWTALMLAAHEGHLPVVQMLVAGKAKTR
                     TKNLRQQTALDIAQAQRAEQVAQVIKLGR"
BASE COUNT         1538 a         2010 c         1903 g         1393 t
ORIGIN      
        1 ggtttcggcg tccatcatca ggctgaaccc gcggcgttcg agaaagcgct tctgtttggc
       61 gtaaccgagg taaccggcgt gcagagcagc agcgcggtaa ccttctttgc caagcacctc
      121 gctcagacac ggcactttaa agtccggcag cacgagcgaa atccaggccc cgtcaggcag
      181 agcgtaggcg ctgcagaacg agttgtagaa ggcattgata ctgagcggaa aattggcata
      241 gtgccgcgta aaaaagaggg accgttcgct cagccggttg agatgcggcg taacttcttt
      301 gtcctgcacc cgcttgccga taactgagaa cggtgtggat tccaggtagt agagaatgac
      361 gttgcgccct tcgatccgct gcacatttac ctgcggtagt gcacgcgtga gttcggccga
      421 ttcgagcgac ggcagcaggt cgttgtctgc caggtcaacc cccaccatag cagtagcttc
      481 agcgcggcgg ctaaaagcag ccgacagtgg gttggcgatc agctggggtt cagctggaga
      541 aagccagtgc aggaccacaa gcagcgtagc cgaagaaaat cccaccgccc agccacgtcg
      601 accgagagca acggcagaac gcgactgcca gaacaggatc gccgcgcagg caacggcgag
      661 tacaaggtgt gcaagaccca gctcatagag tcctgagacg aggttctcct tccagatcgt
      721 cccggtatcg agtcgcacaa aagcgaacga aaaaggacgc tggtagagtc gcgcaaaacc
      781 aaaggcgacc acaaggaaaa ggatgagcac cgtgtaggcc aggcgcacgg gccagcgcca
      841 ggcacgtaag cgcagtgcgg caaagaccgc gcccaagagg ccagcccagc cgcaatcagc
      901 taaaaatgcg tgcaggcagc tgccggccga aaggacgcca atgccggcaa agacgagcac
      961 aaagatatgg taagtggcaa acagggccag ccagagggaa aagaatttca gcacaggtgg
     1021 agattctact ccgaaacgcg ccggcaagcc gcatcggggt tgaacaccgg tctaaaatga
     1081 ttatgtcgta tccggagatc ggccgatatt ctgggtatgc ggtacaagtg gcagaacatg
     1141 aaaaatccgt catgggatta ccccgaaatc tattcgaccg tcactattca ctatgaccag
     1201 ataaaggttg aaaccaaccg cgcgttgggc caggtcaggc tcgaggtgaa aacgctgcgc
     1261 ggcgagcact acgagagtat tattcgccag ggcaccatac tgcgcgaaaa ggacctgcgg
     1321 acgggtgccc tgacggacct taccgatatc ctgagcgtgt accgcaaaga tttcagctcg
     1381 ctgcccgata cccccatact ggcgctgatc ggcggcaact atggcatcat gacggagttc
     1441 atgggtaccg cggagcgcat cgtggttcac gatcgccacc ctttccgcag gttcatgagg
     1501 ctcttacgcg gcatctggcc cgcgcttaaa agatggtacg ggagcacacg cgcaaatttt
     1561 ggctattacc gccatgccgc gcgcaaggcg gacattttcg acgtggcgct gatcggttca
     1621 tccacctatg ctgcctttac ttacaacttc gattacctga atgcgggctt taccctgtgc
     1681 atcgtcgcga tgctaaccgg tcttatcgac tggctgatcc gcaaacgcga accgtggctt
     1741 ctcaaaattt atgcgtcggt cgcgctggga acagtcgcag tctaccgggg ctattttttt
     1801 cagtagcgct gttgattgcg cgcgtaatag atcttcaata cgagaatcgc caccacgagc
     1861 acgagcgtca ccgcgttggc gagtatgatg ggcagactgg acaagagtag cccgtagatc
     1921 agccagagcg tgacgccgag cgcaaaaagc agcatcatcc aaagagagat gtcccgcccc
     1981 gacttgatcc ggtagatgcg tactacctgc ggtacaaatg agatcgttgt gatcataccc
     2041 gcaataaaac ccaaaacctc tgtttccata tcgatcctta gctgatttcg ccaagcacac
     2101 gccgcgcggc catgaccccg cgatactgtg cctcttcaaa attgctcatt cccgaaacgt
     2161 cggaatgggc gccgtagaaa ttctcatcaa tcttttccag taacccacca cgcatgcgcg
     2221 cgagaaatcc cggcgtcggg cgcaccatag cgtgtgccca gcggtagatg catgccgatt
     2281 cgacaaattc gttcacctgg gggtgcgcac ggccgatatc gtcgagcgcc atctgcaaag
     2341 cttcatgctc ggtcatctgc agcaacctgc gcctgctctg cagggtgtca tcttcgtcga
     2401 atgcatggaa aaatgtcagt gtacgtgcat gcggaagttt tcctgcacgc tggtgttcgg
     2461 caacaacgta gcccagcgac tttgaaccga acataacgtt atcccatgca ggggcatttt
     2521 cgccgaaatg attcaccagc aggttcacgg tcagccatgg ggagtaaacc agcgctttgg
     2581 cagcagcacg ctggtcgctg cgcagccctg gataaaccca tggcaggaca aatttgggtg
     2641 cggcgaaaat aacctttcgt gcaatgcaga cttcgacaga ctgggtggca tggttgaaaa
     2701 agtgcagccg gtacccggaa ccggtttttt ccatatgccg cagtgcagtt gccggctgta
     2761 tcgtgttacc cagttttttt cgcaggtatt cggctataaa gccatttccc tcgggccaca
     2821 cgaggttgtg cgcttcgctg cgggccgcga aatagtgcag cagtgcccat gcagatgtgt
     2881 tgtgccggtt ggagccatat tcgtcacgca gtacgtaatc gatataccac aacagccgtg
     2941 gcgaagaaat tccaagctgg tcgaggtagg cggcggcagg gatggcatcg tactgcacat
     3001 actttggatc tgctgaactg agggcgagcg gtatggtaaa agcacggcgg ccgtcacgcc
     3061 cgcgcgcagc agaaaaagct gacatggtgc gaaagaagag ctcgatctgc tgccggtcac
     3121 tctcgcgggc acccatgagc ggtaccagcc ccggctgcca gttgccgtaa atgaagaggc
     3181 gctcctctgt ttcgtgcacc agcgcctctt cgctgaagcg gccgtcctgg tagattccca
     3241 tttctgccag cagttcacgc acatagacgg cgtctgtacc gggtgtcggc aggtagtggg
     3301 ctccccacgg ataacgccca ttgcggtttt cgccccactg cgcattaccg cccgcgcggt
     3361 cttcgagctc gaacaacctg aaatccttca ggccatgctt ctgcagatac cagcctgccg
     3421 acatgccgct gattcccccg ccgatgatgg cgacaggaac ggtcagctgt gccgtgccta
     3481 ccgtggccgt ataaggctcg cggagtttat gcccgcggtc gagcgccggc ccgacaaacc
     3541 tgggcttgta gccggcacga cgcaggaacc accagccgaa tacgctcacc ccggcaaaga
     3601 gtgcagccat cgaggcaata aacccgcggc ggctcaggtc gtcacgattg ccgcctgtct
     3661 tcaggtgcgc cccttaccga gcagaaaaac tgacataccc gcccgcatcg ttgtctcggg
     3721 ggctggtgca tagatcatcg aaccatcggg attctgcagc gcaaccaccg aaaacccctg
     3781 ctcgatatag cggcatgcac cgacattttt gccgacacct tcatcacctg cagtaagccg
     3841 gtgtatcttg acttcaaatt cgtaattgcg gtcgccgcga aaaatctcga tgaaatcgtc
     3901 cacttgtggt ttgagcatcg agcgagccat ctgtatgccg gcaacgtggt atggcgacaa
     3961 gacgcggttc gctcccgcct gtaccatttt ggcacgcgtc gtttcgtgcg aaatacgtgt
     4021 ttcaatccag atggtcgggt tcaggccgcg cgccgataag acgacgaaaa ggttatcggc
     4081 atcggtgggc agaacgctga acaggccgcg ggcacggtcg atacgcgctt cgcggagcac
     4141 ctcttcagac agggcatcgc ccgacaacac tgcccatgcc accttgcgct ccgatacaag
     4201 acgcgagacc ggcgaatcgt tgtttttttc aatcacgaga aaatcacgcc cggccttttc
     4261 cagttcactg cagatggcga gcgccatgcg acctccgccg caaacaatgt agtgcccgtg
     4321 tatgttcctg agtttttcgg agagtatctg tgcgtccata aagcccctga agccgcgttc
     4381 gagaataatc tgtatgactg cgttgatgcc caaaccatag aacatgaccc cgccgagtat
     4441 caggcagatg gtgaagacgc gcccggcatc tgaaagcggc cgcacctcgg aaaaacccac
     4501 cgtggtaatg gaaatcaccg tcatgtaaag tccctcgaaa ggagtaaagc cctcgagcat
     4561 gacataaccg gcgctgccgg taaccagcag caccagcagc accagaatcg gccgcgtaaa
     4621 cagccggatg taatggtata aaagacgaat gcgaagcagt gaacccgggc gctgagccat
     4681 gcgctcacat accccagtca cgcggcgtgc ggaattcacg gccgatagtg acgggagaca
     4741 acttggcgat cacgggcatg ttgcgcttgt acgcattacg ttccacccgt ttcagaatct
     4801 ggtctacgac tgcctcatcc tgcccgtaac ctcgcaccat ttctttcagg gctggcacag
     4861 tatagttgcg ttccatccag tggtacagca ccaggtcggc catggcatag ctgaaaccca
     4921 tttcggcttc atcggtctgg cccgaccaca ggtcggcact cggcgcctta tccagaattt
     4981 cgggaatcac tcccatctgc cgggcaatgg caaacacctg gtatttgtac aggtcaccga
     5041 ggggatttac cgcggaggcc atgtcacccc agagggtcga ataaccgagc aggatttctg
     5101 tcttgttact ggtgccgaca acgagcgaag catcgcgcgc cgaaagatcg tagagcacga
     5161 tcatacgcag gcgcgccatc acgttgccgc ggcgcgtcgc ggtaatggat tcgtcccgag
     5221 aaaagtaagc gtcagccata gcggtgattt ctaccacctc ggtcgctacg ccgaatttgt
     5281 tgcagagctt acgcgcatcg cccagcgaat cgggattact gctcttgtac ggcatcatta
     5341 cagcgagcac gttttctttg ccgagcgcgt tcacggcgag tgccagcgaa agtgccgaat
     5401 cgacaccgcc cgaaactccc aagacggctt tgcgcagccc ggttttttga atttctgccc
     5461 gcagtatctt cgtcagcgcc tctgcggcaa aagcaacgtg aaaatcggga agagctccct
     5521 gttgagacat agggtaatat ctgcaattct cagtcaggct gtcacgcgct aggaactcag
     5581 cggccaagtt tgatgacctg cgccacctgc tctgcgcgct gcgcctgggc aatgtcgagt
     5641 gccgtctgct gccggagatt tttcgtacgt gttttggctt tgccggcaac cagcatctgc
     5701 acaacgggca ggtgcccttc atgcgcggca agcatcaatg ccgtccagcc ctcgttatcc
     5761 tgccggttga cgtcggcccg caaattcacc agtattttta ccaattcggc gtttcctttc
     5821 cacgcagcat gcatgaccgc actgcggccc tgtgcatcct tcgcttcggt attagctttt
     5881 gcctgcacca gcaattccac cacaggcaaa tgccccttca gggtagcgac cagcaatgga
     5941 ctctgcccct ggtggtttaa caggttgacg ttggcaccgc gggcaatcag caaccgggcc
     6001 acttcggcag aaccattagc cgcagcataa aacagcgcgc tgctgccctc gatgtcctgc
     6061 atatcaattc tcagctctgt ggcgcgcagc gccgcctgtg ccagcgctac gttgttggtt
     6121 cgcgcagcct gcgccagttc aagattatta tgaaaatcgg cagagcctga tttcagcaga
     6181 aaatctgcca cctcgtcatg gccgccgagt gccgcccagt acgctgcagt ccctccctgc
     6241 tttgccacgg cattcacatc ggcaccggcc tgtaccagcc gctgcaccgc ctgcaactgc
     6301 ccccggactg cagcgatatg cagaggtgaa aatccgaatc tgctctgcgc attaaccccg
     6361 gcaccggctg cgattagctg gtcgattata cgcacatgat tttcctgtgc ggcgtagaaa
     6421 agagccgtat agccatcggc gtcggtcgaa tggacattgg ccttcagttc gagcagtgtc
     6481 tgcagaattt cagcgttgcc ataagcggcg gcgacaagca gcggcgtatt gccaaattta
     6541 tcggtgtagt tgggatcacc gctgtcatcc atggcgcgga cgaattcggt ggcattattg
     6601 gcagcaatgg cgcgcagcat gcgccggccc gcgacagcat cgaggccgcc gttctgccgc
     6661 aggaattccg cgatctcgcg gtcgttgagc tccactgcgt aagacagcgg tgatttaccc
     6721 tcttcgtctg ccgcattgat gctggcccgc tcggccaaag ctttctgcgc ccccgccagg
     6781 ttgcgatttt ttaccgcgat aaagagatcg cgcgtcgcgt acggattttc agccggcttc
     6841 ttct
//
LOCUS       DAOQJF010000172         6821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6201_length_6821_cov_5.473396, whole genome shotgun sequence.
ACCESSION   DAOQJF010000172 DAOQJF010000000
VERSION     DAOQJF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_6821_cov_5.473396"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..429)
                     /locus_tag="PLF85_09925"
     CDS             complement(46..429)
                     /locus_tag="PLF85_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNE20028.1"
                     /translation="MDQQATLEGLSLRERSEFFRGLLLVMAADGELHDAERELIKHAI
                     SPFDFSAEFVDESIESILKNKHVSQVPPHFESQANSHLFLRAALDIAFADGVLDETEE
                     KWLRATADLNGIAAEWLEEQIAERE"
     gene            554..1918
                     /locus_tag="PLF85_09930"
     CDS             554..1918
                     /locus_tag="PLF85_09930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE20029.1"
                     /translation="MNTRITFLGGAGTVTGSKYLLEHNGKKILIDCGLFQGLKTLRVR
                     NWNPLPLDVSEIDAVILTHAHVDHSGYIPRLIKDGYRGRIYSTPATFDLCKILLPDSG
                     YLMEEEAEYLNKHQRTKHKVALPLFTQQEAEGSLQFFEPVDFNHRVEIAPGIAFEFGY
                     AGHIFGAAQVVLNCDGKRIAFSGDVGRMEDPILYPPARLSGADYLVLESTYGDRLHEA
                     VSPAEILERVICETHARGGVILIPAFAVGRAQQIMYYLWELRRAGRIPEFPMYLNSPM
                     AENASALLTRYSELHRLSPEIAEETCGIVHYVRTAEESRALNERKGPMLIISASGMLT
                     GGRVLHHLKAFAGDARNTILLTGFQAAGTRGEALQRGKREIKVHGEYVQIRAQVTTLE
                     NISAHADYSEILDWLEHCNIGPRRVFVTHGEPVSSDELRRRLIDRFGWHAMVPDDRQT
                     VDLA"
     gene            1948..2406
                     /locus_tag="PLF85_09935"
     CDS             1948..2406
                     /locus_tag="PLF85_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE20030.1"
                     /translation="MALVKSSQEITNSTIGPNSYFTGRFYINGSLKIDGKFEGRALQA
                     DQLYIGTSGKVKTSVSANSVFVEGVIIGNIAAKSRVMLLPTAKIFGDIKTPELIIQNG
                     VILEGRCVIANDLKTSAKDVIEAEYLKDNLNENVLFPKSARPDKEKAEAR"
     gene            2709..3467
                     /locus_tag="PLF85_09940"
     CDS             2709..3467
                     /locus_tag="PLF85_09940"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNE20031.1"
                     /translation="MQKGKAVEPGKGNALVALRSWHAFQKAMKLFQAHPHSALVTLPV
                     SKELIMKGGVDFTGHTEQLAQAFGRKVFMCMYHAKFSVIPLTNHIPLATVPRKLYELP
                     AEELLQALRQFNAIFKPKGKIAWCGLNPHCGENGRVGTEEEFIRRTIDALAAKSMAIE
                     GPISADAVFTKHVLKAYSLVIANYHDQGLIPFKSLNGMAGVNTTLGLPRLRVSPDHGT
                     AFSQTAAKQVDATGVLASLRFALKFGATWQQVSA"
     gene            3446..3802
                     /locus_tag="PLF85_09945"
     CDS             3446..3802
                     /locus_tag="PLF85_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20032.1"
                     /translation="MATGIGISLALGALAMVILTFRSGVALYQKILGWLAFAALAWFY
                     RPELTAAYATLLGGDYRTGAREFANLVQVAMPVVAFTFAWLAFLASSAIDAGRILVFF
                     FVLFLISAAVKLALLP"
     gene            complement(3807..4991)
                     /locus_tag="PLF85_09950"
     CDS             complement(3807..4991)
                     /locus_tag="PLF85_09950"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNE20033.1"
                     /translation="MVKLLLKLPSNQFDGYLSRYSVMPSPLAAQGEFFESRQYPEEPH
                     ALRNPFQRDRDRVVHSRSFRRLEYKTQVFVNHLGDNYRTRLTHTIEVAQIARTVARTL
                     GLNEDLTETVALAHDMGHPPFGHAGERALHEKMKQYGGFDHNEQTLRIVTVLEERYPE
                     FPGLNLTRGTLRSLQKHSSNPQDTAAATIEQQGHCLEALIVDTCDEIAYNNHDIDDGL
                     ESGHLTLQMLENVELWRRHFSAVRQKYPALREKIAIRATIREMINTMVSDLITQSAEN
                     IRHSGIPNFRELLHHTGKAQHAELIAFSESMAVQVIELKKFLFRNLYRHADVVRMNER
                     ANDIISRIFDFVVREPRMLPADFYERIESMGTERVVADFIAGMTDRYALQWNNDILGN
                     RL"
     gene            4990..5430
                     /locus_tag="PLF85_09955"
     CDS             4990..5430
                     /locus_tag="PLF85_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20034.1"
                     /translation="MEQPDFSALAAAMFGTEGKVDLEAADRLWTAVFRLPLWYVLMSP
                     QSAMDNQPSAQLIEEKVWLLAFTDKEKLAHYAARNKNLDAQGNALSLDLAPAQCVELG
                     RTLQHSNVFGFRFNEAQQHGWYIPMTDFLRIPDYLGEKGLLPAP"
     gene            5490..6779
                     /locus_tag="PLF85_09960"
     CDS             5490..6779
                     /locus_tag="PLF85_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNE20035.1"
                     /translation="MKVIRLTAAFIAGIALLATSVHPWSNHYLFNREALAALPEIADA
                     PNVKVESLESFLLKEQVGISELLAKVENEAKESYAKKAPLPEALAFKGGDASNIRANF
                     LRAIRINPGTPLGYFLQQLPGKKLPGKPADPNKVSIFGEKDLKRKYIYYQIKQGDMVS
                     PLEVLAAAGDEPDFGIDINLFVDNNEKFSQDYGFGNQSFGNPKLYYGTQAPFHIGYYH
                     ESGIIFMAAGFLKRTYPRYRVHQFMNLARYAFKTGHPYWGYRFLGWGLHYIGDLTQPY
                     HARVLPNYGTMGMLWINTKAMMGFPTSKDQAIDRITARHTAIEEYHFSVMAAAYAQNN
                     LEHPFLQNLRNTGKDAGYGSFNDDYIVDKLTDESYDLSDKVDDLIDEANLLQGFSEGN
                     VLPEINKKALAELDQVLATHMQGVGAHLRNYVRAGLK"
BASE COUNT         1544 a         1942 c         1866 g         1469 t
ORIGIN      
        1 cgtcaatgag ttgctttttt acggcgcttt agacgccgcg cgggtttatt cgcgctcggc
       61 gatctgctct tcaagccatt cagcagcgat gccgttgaga tctgcagtcg cgcgcagcca
      121 cttttcttcg gtctcatcca gcacgccgtc ggcgaaagcg atatcgagtg cggcgcgcag
      181 gaacaggtgt gagtttgcct ggctttcaaa gtgcggtggc acctggctca cgtgtttatt
      241 cttgagaata ctctcgatac tctcgtcgac aaattctgca ctaaagtcaa acggagagat
      301 tgcatgtttg ataagttcgc gttctgcgtc atgcagctca ccgtcggccg ccattaccag
      361 cagaagaccg cggaaaaact cactccgttc gcgcagagac agaccttcga gggttgcttg
      421 ctgatccata ggttacagtg aaagttaata caagcctacc ggtagggcaa aaaaagtgga
      481 tgaaatcccc attttcgtta aacaaagaag caaacgactt ttcagcgaca gggcattcag
      541 aatcgtgtat tcaatgaaca cccgtatcac attccttgga ggtgccggaa ccgttacagg
      601 ctctaagtac ctgttagaac acaacggcaa aaagatactg atcgattgtg gcctgttcca
      661 gggactcaag acactgcggg tgcgaaactg gaacccactg ccgctggacg tttcagagat
      721 cgacgcggtc attctcacgc acgcgcatgt cgaccattcg gggtacatcc cccgcctgat
      781 caaagacggt taccgagggc gcatctacag cacgcctgcg acttttgacc tgtgcaagat
      841 tcttctgccc gattcgggtt acctgatgga agaagaggcg gagtatctca acaaacacca
      901 gcgcacaaag cacaaggtgg cactacccct ctttacgcaa caggaagccg aaggttcgct
      961 gcagtttttt gaacctgtgg attttaacca ccgcgtcgaa attgcccccg gcattgcttt
     1021 tgagtttggt tatgcaggac atattttcgg tgcggcacag gtcgtcctga actgcgatgg
     1081 caagcgcatc gctttcagcg gcgacgtcgg tcgtatggaa gacccgatac tgtaccctcc
     1141 tgcccggctc tcaggtgccg actatctggt tctagagtcg acgtacggcg atcgcctgca
     1201 cgaagctgtc agcccggcag aaattctcga gcgcgtcatc tgcgagacgc atgcccgcgg
     1261 cggggtcatt ttgatacccg ctttcgcggt aggtcgtgcg cagcagatca tgtattactt
     1321 gtgggagctc aggcgagccg ggcgcattcc cgagtttcct atgtacctca acagcccgat
     1381 ggcagagaac gcgagtgcac tgctgacccg ctacagcgag ctgcaccggt tatctcccga
     1441 gattgccgaa gagacctgcg gcatcgtgca ctacgtgcgg accgccgaag agtcgcgcgc
     1501 actcaacgaa cgcaagggac cgatgctgat catctcggct agcggcatgt taaccggcgg
     1561 ccgagtgctg caccacctga aagcgtttgc cggcgatgcg cgcaacacca tactcctaac
     1621 cggttttcag gctgccggca cccgcggcga ggcgctccag cggggtaagc gggaaatcaa
     1681 ggtacacggc gagtacgtgc agatcagggc acaggtcacg accctcgaaa acatctcggc
     1741 gcacgccgat tactccgaga ttctggactg gctggaacac tgcaacatag gcccgcgccg
     1801 ggtatttgtc acccacggcg aaccggtctc atccgacgag ctgcggcgca ggctcatcga
     1861 ccggtttggc tggcatgcga tggtgcccga tgacaggcag acggtggatt tggcatgacg
     1921 gcctgagcaa aaatctgagg gttacccatg gctcttgtca aaagctcaca ggaaatcacc
     1981 aactcgacga tcggcccgaa ctcgtatttt accggccgtt tttatatcaa cgggagcctc
     2041 aagatcgacg gcaaattcga aggccgcgcg ctgcaggcag accagctcta tattggcacc
     2101 agcggcaagg taaagaccag cgtttcagcc aactcggtct ttgtcgaagg tgtcattatc
     2161 ggcaatatcg cagccaaaag ccgcgtgatg ctgctgccaa ccgccaaaat tttcggcgat
     2221 atcaagaccc ctgaacttat tatccagaac ggtgtaattc tcgaaggccg ctgcgtgatt
     2281 gccaacgacc tgaaaacttc tgctaaagat gtgatcgaag cagaatacct gaaagacaac
     2341 ctcaacgaga acgttctttt ccccaagagc gcgcgccccg ataaagaaaa ggctgaagcc
     2401 cggtagaccc cggcttcgac tggcattcca ttagcgcgcc tggcctacca ccggttatca
     2461 tttcgcaggg cgacgccgct ggcgcgggtt ggtttgcgtt caaagaaatc gtcgctgcat
     2521 ccggaaaata ttttcccgcg ttaaagaaag acggcacgct gcgccggttt gttctcgtcg
     2581 gcgacagctt cgagaacgac gccgatttta ttgccaaaca ctttcacgtt gtaccgtatt
     2641 cggccaaacc tgcagagctc gatacgctgc tgtcagacaa gaagatccgc aaaccgctct
     2701 ttctcagcgt gcagaaggga aaggcagtag aacccggtaa aggcaatgcg ctcgtcgcgt
     2761 tgcggtcctg gcatgctttc cagaaggcga tgaaactctt ccaggcgcac ccgcattcgg
     2821 cgttggtgac gctgccggtt tcaaaagagc tgatcatgaa aggtggcgtc gatttcaccg
     2881 gccacaccga acagctggcg caggcattcg gccgcaaagt cttcatgtgc atgtaccatg
     2941 caaaattttc cgtgattccg ctgaccaacc acataccgct cgcgaccgtg ccgcgtaagc
     3001 tgtacgaact gccggcagaa gagctgctgc aggcgctgcg gcaattcaac gccatcttca
     3061 agccaaaagg caaaatcgcg tggtgtggcc ttaaccctca ctgcggcgaa aatggccgcg
     3121 tgggcaccga agaagaattt atccgccgga caatcgacgc actcgccgcg aagagtatgg
     3181 cgattgaagg cccgatttcg gccgatgccg tatttacgaa gcatgtgctc aaagcatact
     3241 cgctcgtcat cgccaactac cacgaccagg ggctgatacc cttcaaatca ctcaatggca
     3301 tggccggggt gaacaccact cttggtttgc cgcgcctgcg tgtttcacct gatcacggca
     3361 cggcattcag ccagacagcg gcaaaacagg tagacgcaac aggagtgctg gcgagtctgc
     3421 gatttgcact gaagttcgga gcaacatggc aacaggtatc ggcataagtc tggcattggg
     3481 ggcgctcgcg atggtcatat tgaccttccg cagcggagtt gccctgtacc aaaaaatact
     3541 gggctggctc gcgtttgccg cccttgcctg gttctaccgc cccgaactga cggccgctta
     3601 tgcaaccctg ctgggcggcg attaccgcac cggtgcgcgt gaattcgcga acctggtcca
     3661 ggtggcgatg cccgtagtcg cattcacttt cgcctggctg gcattcttag cctcttcagc
     3721 catcgatgcc gggcgcattt tggtgttttt cttcgtgctg tttctgatca gcgcagcagt
     3781 caagctcgct ctgctgcctt aaaaactcaa agccggtttc ccaggatatc gttgttccac
     3841 tgcagcgcgt aacggtcagt catgcccgcg ataaaatcgg cgacaacgcg ttcggtgccc
     3901 atgctctcta tacgctcata gaaatcagct ggtagcatac gcggttcacg cacgacaaaa
     3961 tcgaagatgc gcgagataat atcgttggcg cgttcattca tgcggacgac atctgcgtgg
     4021 cgataaagat tacggaacag gaatttcttg agttcaatga cctgcacggc catactctca
     4081 gaaaatgcga tgagctccgc atgctgcgcc ttgcccgtat ggtggagtag ctcccggaaa
     4141 ttggggatac ccgaatggcg gatattctcg gccgactggg taatgaggtc agaaaccata
     4201 gtgttgatca tctcgcggat cgtcgcgcgg atcgcaatct tctcgcgcag cgcaggatat
     4261 ttctgccgca cagcggagaa atgccgtcgc cacagctcga cgttctcaag catctgcagg
     4321 gtcaggtgtc cgctctcgag cccatcgtcg atgtcgtggt tgttgtaggc aatttcgtcg
     4381 caggtgtcga cgatcagcgc ttcgaggcag tgcccctgct gttcaatggt agctgcggca
     4441 gtgtcctgcg gattgctgct gtgcttctgc aggctgcgca gggtaccgcg cgtgagattc
     4501 agccctggaa actcggggta ccgttcttcg agtacggtga caatgcgcag tgtctgttcg
     4561 ttgtggtcaa agccgccata ctgcttcatc ttctcgtgca gcgctcgctc ccccgcatgg
     4621 ccaaacggcg ggtgacccat gtcatgcgcc agcgcgacgg tctcggtcag gtcttcgttg
     4681 aggcctagcg tgcgtgccac ggtacgggca atctgcgcca cctcgatggt gtgcgtcagc
     4741 cgggtgcggt agttgtcacc gaggtggtta acaaacacct gcgtcttgta ttcgagccgg
     4801 cgaaacgaac gcgagtgcac cacgcggtcg cggtcacgct ggaaggggtt gcggagtgca
     4861 tgcggttctt cggggtattg acggctctca aagaactctc cctgtgctgc aagcggcgaa
     4921 ggcataacag agtatcgact taagtagccg tcgaattgat tcgagggtaa tttaagcagc
     4981 agtttgacca tggaacaacc cgatttttct gcactcgccg cggcgatgtt tggtaccgaa
     5041 ggaaaagtgg atctcgaggc cgccgaccgg ctctggaccg ccgtatttcg cctgccgctc
     5101 tggtacgtgc tcatgtcgcc gcagagtgcg atggacaacc agccgtcggc gcagctgatc
     5161 gaagagaagg tctggctgct agctttcacc gacaaagaaa agctggcgca ttatgcagcg
     5221 cgcaataaaa acctcgatgc gcagggtaat gcactctcac tcgacctggc gcccgcgcag
     5281 tgcgtcgagc tgggccgtac cctgcagcac agcaacgtgt tcggctttcg ctttaacgag
     5341 gcgcagcagc acggctggta tattcccatg acggattttc tgcgcatacc tgactacctc
     5401 ggcgaaaaag gcctgcttcc agctccttaa gaatgggtca tccgcattca taacacttga
     5461 ctgcgcggtc aggtgatcag gattacgtta tgaaagttat ccgcctgacc gccgcgttta
     5521 tcgccggtat tgctttgctg gcgacctcgg tgcatccgtg gtcgaaccat tacctgttca
     5581 accgcgaggc actcgccgca ctgcccgaaa tcgccgatgc gcccaatgta aaggttgaat
     5641 cgctcgagtc ctttttactg aaggagcagg tgggtatcag cgaactgctg gccaaagtcg
     5701 aaaacgaggc caaagaatcg tatgctaaaa aagctcccct gccagaagcg cttgccttca
     5761 agggtggcga tgcttctaac attcgcgcca actttttgcg tgcaatacgc attaaccccg
     5821 gcaccccgct cggctacttt ctgcaacagc tacccggtaa aaagttgccc ggcaagccag
     5881 cagacccaaa caaggtgagt atcttcggcg agaaagacct caagcgcaag tacatttact
     5941 accagatcaa gcagggtgac atggtgagcc cgcttgaggt actcgctgct gcaggcgatg
     6001 aacccgactt tggtatcgac atcaacctgt tcgtcgacaa caatgaaaag ttcagccagg
     6061 attacggttt tggtaaccaa tctttcggca acccaaagct gtattatggc acacaggcac
     6121 cgttccacat tggctattac catgaatcgg gaatcatctt catggctgcg ggttttctca
     6181 agcgcaccta cccacgctac cgcgtgcacc agttcatgaa cctcgcgcgc tacgccttca
     6241 aaaccggcca cccgtactgg ggctaccgct ttctgggctg gggcctgcac tatatcggcg
     6301 acctgacaca gccctaccac gcgcgcgtgc tgccaaacta cggcaccatg ggtatgctct
     6361 ggatcaacac caaggcaatg atgggttttc ctacatctaa agatcaggcc attgaccgta
     6421 tcaccgcgcg ccacacggcg attgaagaat accacttcag cgttatggct gcagcctatg
     6481 cgcagaataa cctggagcac ccgtttctgc agaacctgcg caacactggc aaagacgccg
     6541 gatacggttc atttaacgac gattacattg tcgataagct gacggacgag agttatgacc
     6601 tctcggacaa ggtcgatgac ctgatcgacg aagcgaacct gctgcagggt ttttcagagg
     6661 gcaatgtact gccagagatc aacaagaaag ccctggccga actcgaccag gtactcgcga
     6721 cgcacatgca gggcgtcgga gcccacctgc gcaactacgt ccgcgcaggg ctgaagtaga
     6781 tttgcctgcg gcaaatctac ttcagagatc cagctcttct a
//
LOCUS       DAOQJF010000173         6698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6355_length_6698_cov_6.353756, whole genome shotgun sequence.
ACCESSION   DAOQJF010000173 DAOQJF010000000
VERSION     DAOQJF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6355_length_6698_cov_6.353756"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLF85_09965"
     CDS             complement(<1..235)
                     /locus_tag="PLF85_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE20036.1"
                     /translation="MADLLHEALAGEKFAIAKLISYFESARARDLEECLRVDRDIAQH
                     STKQGLLVGLTGAPGAGKSTLIQHLVDSLLTLRP"
     gene            complement(228..1172)
                     /locus_tag="PLF85_09970"
     CDS             complement(228..1172)
                     /locus_tag="PLF85_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE20037.1"
                     /translation="MAAEVLQADRRFIAGLLFTILGAAGFSAKAILAKLCYRYGISGF
                     ATLTLRMIFSLPFYLVIFFWFYRLQSPELRAGFRKHWAAMGLVGLSGYYLASWFDFVG
                     LEYISASFERLLLFIYPTLVLLISLFWLKTRAGRREIAALLVTYIGIGISYHNETGAV
                     GSKAHIGAIYIVFAAITYAIYLVATEKLARHYSPLVFTSFAIIISAIAIILHGALAGE
                     KLLGHPAPVYWYILVMAIFCTVLPSLLVAQGIALIGSNRAAILSTLGPVMTIYLAYTV
                     LGDEITVAQTIGTICVLAGIALLTIRGGKKKEKVEQHG"
     gene            complement(1162..2856)
                     /locus_tag="PLF85_09975"
     CDS             complement(1162..2856)
                     /locus_tag="PLF85_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE20038.1"
                     /translation="MRHSIFAVFLALISFAGCGKPKEWTERYKRSARADIDLYLVPEK
                     YALLESSWLKKTAIARFDKEQLAQSLAQYGRSVDDLKRNPAYEYKEEGNVVKLRVGRI
                     SHSTAYDYDVNIPLVFSGKWFDAGQFGEVVYQQHIAPTLAKLLGVSAPSGVEVRPLGV
                     RTDVPKKPEIVVVAVIDQGGLNLLSTHRSAAPRIHALMQKSANYTNAQVGHLDAHTAV
                     GHMAIGTGGFPVKSGVIGNTFFSMERKAGKEKLNKAEIYATKDESRVSPEELKSESLA
                     DVLNVVNKGRSVIVSQSYALRAALGMAGHGASLSAPDIAAAVRTGNLVYWLDAGKSRW
                     ITDARYYSLPAVAKASEPLLTYAKYYPQGYEGYAIRDTKAAYENWGVMMSTPAETQLE
                     GELIRAVVQQEIIGKKKHNDGATDLVYVSFKSADAVGHHFGYHSLEARETLAEIDNQI
                     GKIEDFLRQNYGEDFVLVLTADHGCAPLSEITGGARLTVEEVITEIDKLLPAEVAAND
                     SLVTFMTVGQVSLNKKLMAAHNVTLEQVRARIMTIKPDGKTFFKDVLTRRDLDLGG"
     gene            complement(2989..3837)
                     /locus_tag="PLF85_09980"
     CDS             complement(2989..3837)
                     /locus_tag="PLF85_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE20039.1"
                     /translation="MPYFPNATSDLYYRTAGKGKTALILIHGWYQNGQQAYSAILPHL
                     REKYHVFIPDLPGHGLTPHIPADFSTTMNENLLLEFARYIRQHYRCQKVFIAGHSYGA
                     FSVLAMVSRMPAELDGVIALSAVDDYAPYHKRLKSVLRIPRLLTPIYYRLQALLGGFP
                     YGDREQLYGDAHQELKPGKLAYAKKKNKTLPPAASRRYMLAFLSARVHWPAGQIQTPL
                     LLVYGSRDMLTPASWAKKIQPHFKRSSVEVVADAGHNVQISAPKAVAGHVIKFIQLNT
                     GKPRRR"
     gene            complement(3887..3973)
                     /locus_tag="PLF85_09985"
     tRNA            complement(3887..3973)
                     /locus_tag="PLF85_09985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3937..3939),aa:Ser,seq:gga)
     gene            4078..5547
                     /locus_tag="PLF85_09990"
     CDS             4078..5547
                     /locus_tag="PLF85_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20040.1"
                     /translation="MHIHRLAVVLLLANTLPILSQAPTAAPVQPSLAIVALPDYVDAT
                     GSKNYTYLGPSLTDAINNSMQERFDFQRADKAAVDREVKKLWRPGKIPLDSDVKQIAI
                     LTRSDYVIVGSYTLNPRKTQVTFATRVFVAPDRFIQVPDVTNAVDATLFDATNKVAAE
                     IVRVIEADARAQQAKLAAAAPAGKPGEKIALAKAAEAPPPPKPEPAKKSEPVDDEVEV
                     FHDRNAVYAGISYQGNYFERHTQFYRYASTMTAIDAGLRLGGLNIYGQIGMPSTPQGS
                     SIPTLNKNSGFWLIGLESQSHFGSGWANYGVNYRSFSEPNPVGIQGRDLLKWSSTLYA
                     DTGFHMFNIGPVAFGLTFATYLDIMNAKHPAKSTDDLLMLWNLEGGLYASYLLPVIRA
                     RLRFGADTSYAIFDQTSETYIVSTFGDVELDGKSRTGKWSGMRYSLSLSRSFMGIMLR
                     AQYFTYPSIQTAFQPVDGQNKPMNSASYLRFSADYQFGF"
     gene            complement(5579..6427)
                     /gene="truB"
                     /locus_tag="PLF85_09995"
     CDS             complement(5579..6427)
                     /gene="truB"
                     /locus_tag="PLF85_09995"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNE20041.1"
                     /translation="MDKAAGVTTRLAVKNVLQVKGRGFGVEGILDPFASGLLIAATGD
                     STRFLSYFLRFEKTYVAELTLGRETDTLDNMGSLTKEMPVPRLLVEVLAGTKQQFQGK
                     ILQVPPLFSNVQVDGRRGHQIARAGETAELKPREVTIHSLDLRILNDRTLRMECTVSA
                     GTYIRSLARDIAAALGTCGHLSALRRTAIGPFLVVTKEAPTSQYGEILQSLADADALG
                     SFAALHLPLDVARRFYQGQRPAVAPDTEHGLRRVFAEDRFIGLGRLEQGRLLVENIYP
                     QASLGF"
     gene            complement(6451..>6698)
                     /locus_tag="PLF85_10000"
     CDS             complement(6451..>6698)
                     /locus_tag="PLF85_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNE20042.1"
                     /translation="KKDIDAVLETLTEIAYKEAKSVGKFTLPGFGILKLVQRAARMGR
                     NPATGEQIKIPAKTVVKFSVAKAAKEAIAGKAPAKKK"
BASE COUNT         1532 a         1888 c         1842 g         1436 t
ORIGIN      
        1 ctgggcgcaa cgtgagcaga gaatcgacca agtgctgtat cagggtagat tttccagccc
       61 ccggcgcgcc ggttaaaccg acaagcaaac cttgtttggt cgaatgctgg gcaatgtcgc
      121 gatcgacgcg caggcattct tccaggtcgc gcgcgcgcgc agattcgaag tagctgataa
      181 gtttcgcgat cgcgaacttc tcgcctgcaa gcgcttcgtg caaaaggtca gccatgctgc
      241 tcaacctttt ctttcttttt tccgcccctg atagtcagca gtgctatacc ggcgagcaca
      301 cagatcgtac caatggtctg cgcgacggtg atttcatcgc cgagcacggt atacgccagg
      361 tagatcgtca tgacaggtcc cagtgtgctt aagatcgccg cgcggttaga gccaatgagc
      421 gcgatgccct gcgcgacaag cagcgatggc agcacagtgc agaagatagc catgaccagg
      481 atataccagt agaccggcgc ggggtgcccg agcaattttt cacctgccag ggcgccgtgc
      541 agaatgatcg caatcgcaga tatgataatg gcgaaactgg taaagaccag cggcgaataa
      601 tggcgggcaa gtttctcggt cgcgaccagg tagatcgcat aggtaatcgc cgcgaataca
      661 atgtagatgg cgccgatatg cgccttgctt cccaccgcgc cggtttcatt atggtaagaa
      721 ataccaatac cgatgtaggt caccagcagc gcagcaattt cccgccggcc cgcacgcgtc
      781 ttgagccaga acagcgaaat cagcaggaca agcgtcgggt agataaagag caacaggcgc
      841 tcaaagctgg cggagatata ttcaaggccg acaaaatcaa accagctagc aaggtaatac
      901 cccgacaacc ccacgagacc catcgccgcc cagtgctttc gaaaacccgc acgcagctca
      961 ggtgactgca gccggtagaa ccagaagaat atcaccaggt aaaacggcag ggaaaaaatc
     1021 atgcgcagcg tgagcgtggc aaagccgctg ataccgtaac ggtagcagag tttcgcgaga
     1081 atcgccttcg ctgaaaaacc cgcggcacca agaatggtga agagcaggcc tgcaatgaac
     1141 ctgcggtcag cctgcagaac ttcagccgcc aagatcgagg tccctgcggg tcagaacatc
     1201 cttgaagaag gttttaccgt cgggtttgat ggtcataatg cgcgcgcgta cctgctcgag
     1261 cgtcacattg tgtgccgcca tgagtttctt gttgagtgaa acctggccca cggtcatgaa
     1321 ggtaaccagt gaatcattcg cagcgacttc agccggcagc agcttgtcaa tttcggtaat
     1381 aacctcttcg actgtcaggc gggcaccccc ggtaatttca gaaaggggag cacaaccgtg
     1441 atcggcggtg agcacaagga caaaatcttc accgtaattc tgccgcagga aatcttcgat
     1501 cttgccgatc tggttgtcga tttcagcgag agtttcacgc gcttcgagcg aatgataacc
     1561 gaagtggtga ccgaccgcat cggcagattt aaacgacaca tagacgaggt cggtggcacc
     1621 atcattgtgt tttttcttac caatgatttc ctgctgcacg actgcccgaa taagctcccc
     1681 ttcaagctgt gtctctgcag gggttgacat catcactccc cagttctcat aagctgcctt
     1741 cgtgtcgcgg atggcgtaac cttcataccc ctgtggatag tactttgcat aggtgagcag
     1801 cggctcagat gcttttgcca cggcaggcag cgaataatag cgcgcgtcgg ttatccagcg
     1861 cgatttgccg gcatcgagcc agtaaaccag gttgcccgtg cgcaccgcgg cggcgatatc
     1921 aggcgccgaa agcgaagcac cgtggccggc catgccgagg gcagcgcgca gcgcgtaaga
     1981 ctgcgaaaca atgacggagc gccccttgtt cacgacattg agtacgtcag caagactttc
     2041 cgatttgagt tcttcgggtg aaaccctgct ttcgtctttg gtcgcgtaga tttctgcctt
     2101 gttgagcttt tccttaccgg ccttgcgctc catcgaaaag aaagtattac cgatcacgcc
     2161 gctcttcacc ggaaacccac cggtgccgat ggccatatga ccgacagcgg tgtgcgcatc
     2221 gaggtggccg acctgcgcgt tggtatagtt cgccgatttc tgcatcagcg cgtggatacg
     2281 cggtgctgca gaacggtggg tgctcagcaa attcaaaccg ccctggtcga tcacggctac
     2341 cacaacaatc tcgggcttct tgggcacgtc ggtgcgcacg ccgagtggtc gtacctcaac
     2401 tcctgacggg gcactgacac ccagcaactt tgccaaagtg ggagctatat gctgctggta
     2461 gacgacctcg ccaaactggc cggcatcgaa ccattttccg ctgaaaacaa gcggaatatt
     2521 cacatcgtag tcataggccg ttgagtggga aatccgcccg acgcgcagct tcaccacgtt
     2581 gccttcttct ttatattcgt aagccgggtt acgcttgagg tcatcgaccg agcggccata
     2641 ctgtgcgagc gactgcgcca gttgctcttt gtcaaaacgc gcgatcgcgg tttttttcag
     2701 ccagctcgac tcgagcaggg cgtacttttc ggggaccagg tagaggtcta tgtcggcacg
     2761 cgccgaacgt ttgtagcgct cagtccattc tttgggtttg ccgcaaccag caaatgagat
     2821 gagagccagg aagacggcaa aaatactgtg gcgcattatc caccccactt tcgccgccag
     2881 caccgcaaag tactttcaga aggaggagct gaacttacaa ccccgcttcg ggtctccgac
     2941 ttcggagctt atcgaacggt tcgcgggaat gacgtaattc gaagaattct agcgtcgccg
     3001 cggcttgccg gtatttagct gaatgaactt tatcacgtga cccgcaaccg ccttcggtgc
     3061 cgagatctgt acgttatgac cggcatcggc aacaacttcg accgagctgc gtttgaaatg
     3121 tggttgaatc tttttcgccc aggaagctgg cgtcagcata tcacggctgc cgtagaccag
     3181 tagcagagga gtctgtattt gtcctgcggg ccagtgtacg cgtgcagaga gaaaagcaag
     3241 catgtagcgg cgcgaggccg ccggaggcag cgtcttgttc ttcttctttg catacgccag
     3301 ctttcccggt ttgagttctt ggtgcgcgtc accatagagt tgctcgcggt cgccgtacgg
     3361 aaaaccgccg agcaacgcct gcaaacggta atagatcggc gtcaacaaac ggggaattcg
     3421 cagcacgctt ttcagacgct tatggtaggg agcatagtcg tcgacggccg aaagagcaat
     3481 aacgccgtcg agctccgccg gcatacgcga aaccatcgcc aggaccgaaa acgctccgta
     3541 cgaatggcct gcgataaaaa ccttctggca gcggtaatgc tgccgtatgt aacgcgcgaa
     3601 ttcgagcagc aggttctcgt tcatcgtggt agaaaaatct gccggtatat gcggggtgag
     3661 cccgtgtcct ggcaggtcag gaataaaaac atggtacttc tcgcgcagat gaggcaagat
     3721 agccgaataa gcctgctgcc cgttctggta ccagccgtgg ataagaatga gcgcagtttt
     3781 gcccttaccg gcagtgcggt agtagaggtc agatgttgcg ttgggaaagt agggcataaa
     3841 atgactttga tcggtgcgcc gcgcgcaccg atcaaagctt agactgcgga gtgggtggga
     3901 ttcgaaccca cgatacagtt tcctgtatac agcatttcca atgctgctcc ttcaaccact
     3961 cggacaccgc tccagcgtgg tagcagacgc gaaggttgag acatcgcgtc gagcaggttg
     4021 tgataccgtg agtgcagcct tggttgaccc actgtgtcaa gctcctgttt gaccgcaatg
     4081 cacattcacc gcctcgcggt ggtcctgcta ctggcaaaca ccctaccgat cctgagccag
     4141 gcaccgactg ccgcccccgt acaaccatca ctcgccattg tcgcgctgcc cgactacgtc
     4201 gacgcgacgg gttcaaagaa ttatacctac ctgggccctt ccctgaccga cgcgatcaac
     4261 aactcaatgc aggaacgttt cgacttccag cgcgccgaca aagccgccgt cgaccgcgaa
     4321 gtgaagaaac tctggcgtcc cggtaagatt ccactcgaca gcgacgtgaa gcagattgcg
     4381 attctgacgc gcagcgacta tgtcatcgtg ggttcgtaca cgctcaaccc ccgcaagacg
     4441 caagtcacat ttgccacacg tgtgtttgtc gcgcccgatc gttttataca ggtaccggat
     4501 gtgaccaacg ccgtcgacgc aactctattc gatgccacca acaaggtggc ggcagagatt
     4561 gtgcgcgtga ttgaagccga cgcacgggcg cagcaggcga agctggccgc agccgccccc
     4621 gcgggcaagc cgggcgagaa gattgcactc gccaaagcag cagaagctcc cccgccgcca
     4681 aaacccgaac cggctaaaaa atcagaaccc gttgatgacg aggtcgaggt ctttcacgac
     4741 cgcaatgcag tttatgcagg tatcagctac cagggtaact attttgaacg ccatacacag
     4801 ttctatcgtt atgcaagtac aatgactgcc attgacgcag gcctgcgact gggtggcctg
     4861 aatatctatg gccaaattgg tatgccttcg acaccccagg gcagttcaat accgacgctg
     4921 aacaagaact ctggcttctg gcttatcggg ctcgaaagcc agagccattt tggttctggt
     4981 tgggcgaact acggcgtgaa ttaccggtcg ttcagcgagc ccaaccccgt gggtatccag
     5041 ggccgcgacc tgctcaagtg gtcgagcacc ctgtatgccg atactggctt ccacatgttt
     5101 aacatcgggc cagtggcatt tggattaacc tttgccacgt acctggacat catgaacgcc
     5161 aaacatccag cgaagtcgac agatgacctg ctgatgctgt ggaacctcga gggcggtctt
     5221 tacgcttcgt acctgctgcc cgtcattcgc gctcgcctaa gatttggtgc agatacctct
     5281 tacgcgattt tcgatcaaac ttcggagacc tacattgttt caacctttgg cgatgtggaa
     5341 ctcgacggta agagcaggac aggtaaatgg tcaggcatgc gctacagcct gtcgctcagc
     5401 cgcagcttta tgggtattat gctgcgcgcg cagtacttta cttacccaag catccagaca
     5461 gccttccagc cagtcgacgg ccagaacaaa ccaatgaact cggccagcta cctgcgcttc
     5521 tctgccgatt accagtttgg gttttagaag gctgttgaaa acagccatga gcaggcgctc
     5581 aaaagcctaa acttgcctgt gggtagatat tctctaccaa caggcggccc tgctcgagcc
     5641 tacctaagcc gataaagcgg tcctccgcga aaacccggcg caggccgtgc tctgtatcgg
     5701 gcgctacagc aggtctttgt ccctggtaga aacgtcgcgc cacatcgagc ggtaaatgga
     5761 gtgctgcaaa agatcccagc gcgtcggcat cagcgaggct ctgcagaatt tccccatact
     5821 ggcttgtggg agcttcttta gtaactacta agaacggccc aatggcagtc cgtcgcagcg
     5881 ctgacaggtg gccgcaggta cccagcgcgg cagcaatgtc ccgcgccagc gaccgaatat
     5941 acgtgcccgc gctaaccgtg cactccatgc gcagggtccg gtcgttcagt atccggagat
     6001 cgagactgtg tatagtgact tcgcgcggct tcagctctgc agtttcaccg gcccgggcga
     6061 tctggtggcc ccggcggccg tcgacctgca cgtttgagaa tagcgggggc acctgaagga
     6121 ttttcccctg gaactgctgt ttagtacctg ctaaaacctc gaccaaaagc cggggaacgg
     6181 gcatttcctt agttaaggaa cccatattgt ccagcgtgtc ggtctccctt cccagggtca
     6241 gctccgccac ataggtcttt tcaaagcgca aaaagtaaga gaggaaacgg gtagagtcgc
     6301 cggtcgcggc gattaacagg ccagaagcaa aagggtcgag gataccctcg accccaaatc
     6361 cgcgcccctt tacctgaagg acgttcttca ctgcgagacg ggtggttacg cccgcagctt
     6421 tgtcgataca atagagaatg gtttagcgga ttattttttc tttgccggcg ctttgccagc
     6481 gatagcttct ttcgcagctt ttgctacaga gaatttcacg acggtttttg cgggaatctt
     6541 gatctgttcg ccggttgccg ggttgcggcc catgcgcgct gcgcgctgta ccagcttcag
     6601 gataccaaag cctgggagcg tgaatttacc gaccgatttc gcctctttgt aagcgatttc
     6661 tgtcagtgtt tcgagaactg cgtcaatgtc cttcttgc
//
LOCUS       DAOQJF010000174         6660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6413_length_6660_cov_4.680242, whole genome shotgun sequence.
ACCESSION   DAOQJF010000174 DAOQJF010000000
VERSION     DAOQJF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6413_length_6660_cov_4.680242"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1335
                     /locus_tag="PLF85_10005"
     CDS             175..1335
                     /locus_tag="PLF85_10005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE20043.1"
                     /translation="MRVLDRLLLGIAGLSLLLMVFIFFSDESHLSARPLTSQRNVREG
                     REMPVTLNNTADHLELKSADVDAALERYFDEIRKNPERGEEDLRLVDELVPEDEPQQL
                     ENAVTAEAPAAEPAKPAPPAKKEAAPVKKSERVQVVKHEVRPGESIWRLAKKYNVSIT
                     TIVNANKIKEKTVIQPGDSLRIPDRTGVFHKTAKKESLSDLARRYKVSPETVKRANGI
                     AGNLVPPGKEIFLPGAKPLPEIQYVKQKVFAWPIRAKNRLTSGFGWRVHPITGSRAFH
                     TGIDIGASKGTPILAAADGVVVFSGDGGSYGNMVILRHKNGLYTVYGHASKLIAKKGK
                     YVKRGQKIALVGSTGASTGPHLHFEVKGTEKHVNPAIALKKTERVAVKRRQA"
     gene            1332..2480
                     /locus_tag="PLF85_10010"
     CDS             1332..2480
                     /locus_tag="PLF85_10010"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE20044.1"
                     /translation="MNRIPFARAPVTFADAAPFLQQALDNGWLTTGPLVQQFTAQVAE
                     FLGTSTLIPVSSCTAALQIVLAAADFSPGDEVIVPANTFIATFETVELANLKPVLCDI
                     DAETWNLDIHAAAKLINPRTRAIIAVPFAGNPLPMEPLRALCDKHQLRLILDNAHALE
                     TKWNGKNLHHYADYSCYSFYATKNLTTAEGGAIVCREAEAERIRALSLHGMSRAAWNR
                     YAGGTWRYDIVETGFKANLTDIHAAIGLAQLPKLAEHHERRRYLALRYRNALKDLPVR
                     VQQIDEGGSDTDHAWHLFCISVRPEASVTRENLMEALAAAGIGYSVHFIPVFEFSNTQ
                     RKYNFPSGEFPATTGYFTGALSLPLYPTLADADQDEVIRVLRAAFPGT"
     gene            complement(2443..4140)
                     /locus_tag="PLF85_10015"
     CDS             complement(2443..4140)
                     /locus_tag="PLF85_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNE20045.1"
                     /translation="MTRKLLEELESSQNGSFELTEEKGKAVLRIFNPTGKGRAVHPTD
                     LNNRLRSMHIVPDKNVSVERLLRSTQEGKIAEGEAHVIGDWPKVAQRKDAVAEVEVSD
                     DHMSAHLFLQPPEGGGKAIPEERLRALLREHGVIKGLLDDTLRELGVKPSYKRMILVA
                     QGKEPVRGKNGFIQPLFETMTRPRVDPKAQKIDHKQVNLIKSAKAGEVIAEKIDPTVG
                     ETGFTVGGKLLPAEPGVIAEFIPGANVEISPDGKKLISKIEGRPVLESNGKIRVDEVV
                     YLKNIDYSTGNVDFPGSVIVEESVADGFKLHASGSIILQSSVGVCEITAGKDIILSAG
                     FMGRGEGKITSDGDVYARFVEQGTIIAKGSIFISEATLHSHLVAGGSIVVKGGRGDIT
                     GGDISAGKFVHCNKLGGAGETRTVLTVGIDPTMRSAIDEIHASLKEKEVTLEKIRVSL
                     NRLNDAMKKRKLDAKEAETREKLLLAMRKYKALVESDQRQLESAQNSYLAHDRAYVLV
                     ENQLYPNVEINFGKGMLFRSGMRAVQGKQAIYVGEDRTVIAAPTLPKYLEKQHEEHG"
     gene            complement(4165..5229)
                     /locus_tag="PLF85_10020"
     CDS             complement(4165..5229)
                     /locus_tag="PLF85_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE20046.1"
                     /translation="MVSALRQLYLMHLREFFRDWEIILWSVLLPVAMSWVLGIAFVQK
                     KVTTRQIAVVGDLRQSSELREVINEIENRNISRPKKTNDHSVFRFSFTDQENALRLLR
                     KGKVVLFIELSGEGVVRFHLDPANETSYLSYLILSRRLSQERVEMQLTPVTTHGSRYI
                     DFLIPGLIALGIMNSCLWGIGYVLIEYRMKKLMRRMIATPVSRAAILASFFLSRMTIN
                     FVEALLLFLFGYLYFGFQLQGDIGSILLLFISGNIAFAGLAFLLASRAENTRTGNGVI
                     NVFSIPLMLASGIFFSYTNFPEAIQPLLQYSPLALLADALRTVFNSSGEWREILIPAA
                     ILNAFGVFCFSISLKIFRWH"
     gene            complement(5222..6091)
                     /locus_tag="PLF85_10025"
     CDS             complement(5222..6091)
                     /locus_tag="PLF85_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE20047.1"
                     /translation="MNDLSLEIKEGEFVALLGPNGAGKTTLLEMIEGLQTPDSGSIEI
                     CGLNYAQHERQIRQIMGLAFQETRFPEKIRVAEALRLFAAFYGASPARTVEILDLVSL
                     AEKKNAYVNTLSGGQRQRLALGIAIIQKPKVLLLDEPTTGLDPHARRELWQILSDLKR
                     AGMTLLLTTHYMEEAEELCERIVIQFRGRILADGALEDLLEAHASHEIIEFRLKKVQA
                     RQIAGLRQMKKVSYFNIDEQTGSGVMHVDDAATVLPDFMRRAGRQNLESFAARPLTLD
                     DLFIKMTGRHLDG"
     gene            complement(6165..>6660)
                     /locus_tag="PLF85_10030"
     CDS             complement(6165..>6660)
                     /locus_tag="PLF85_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNE20048.1"
                     /translation="EAIKIAHKRKVFVAVDPQVKHFKQYLECDIMTPNEKEASEGIGM
                     PFPQSDEEVHTIATKIRETLRLPHLLITRSQRGMAYFTDKKSAYLPTVAREVFDVTGA
                     GDTVISVYTAAICAGAEHTEAMVLSNLAGGIVVGKLGTATVSTAELGNEIGRPLPQLR
                     EVGS"
BASE COUNT         1484 a         1925 c         1765 g         1486 t
ORIGIN      
        1 gacatgccag ggcacccccc tgcgctcgag ctccccggcg agcatcaggc cagcaatacc
       61 cccgcccaga atatgtacca tgaaccggcc tttcgccggc tggtacggcg acaaacagga
      121 tacaattttc gcttaggtgt taaataggcc ggttacgatg ccgaaaataa gacagtgcgc
      181 gtactggaca gactgctctt aggtattgca gggctttcgc tgctgctgat ggtttttatc
      241 ttttttagcg atgaatcgca cctgtcagcg cgcccgctca cctcgcagcg caatgtccgc
      301 gagggtcgcg aaatgccagt gacgctcaac aataccgccg atcaccttga actcaagagc
      361 gccgacgtcg atgcagcgct tgaacgctat tttgacgaga ttcgcaaaaa ccccgaacgc
      421 ggcgaagaag acctgcgcct cgtcgatgaa ctcgtgcctg aagatgaacc ccagcagctg
      481 gaaaatgccg ttacggcaga agcgcctgcg gccgaacccg caaaacccgc cccaccggcg
      541 aagaaggaag cggcacccgt aaagaaatcg gaacgcgtgc aggtcgtcaa acacgaggtt
      601 cgccccggcg aatcgatctg gcgcctcgct aaaaaatata atgtcagcat tacaacgatc
      661 gtcaacgcta acaagattaa agagaagacc gtcattcagc caggcgacag cctgcgtatt
      721 cctgaccgga ccggcgtctt ccacaagacg gcaaaaaaag aaagcctgtc agacctcgcc
      781 cgccgctaca aggtgtcgcc cgaaaccgta aaacgtgcca acggcatcgc cggcaacctc
      841 gtcccgcccg gcaaagagat attcctgccg ggagcaaagc cactgccgga aatccagtac
      901 gtcaagcaga aggtctttgc ctggccaata cgcgcaaaaa accgtctgac atcaggtttt
      961 ggctggcgcg ttcatccgat tactggcagc cgtgctttcc acacgggtat cgacatcggg
     1021 gcttccaaag gtacccctat ccttgccgca gctgatggcg tagttgtgtt ctccggcgac
     1081 ggcggcagtt atggtaacat ggtaatcctg cgccataaga atggtctcta tactgtctac
     1141 ggccacgcga gcaagctgat cgccaaaaaa ggcaagtacg tgaagcgcgg acagaagatc
     1201 gcactggtcg gctccaccgg cgcatcaacc ggaccgcacc tgcactttga agtcaagggc
     1261 accgagaaac acgtcaaccc tgctattgcg ctcaaaaaaa ccgagcgcgt cgcggttaag
     1321 cgccgccagg catgaaccgc attccgtttg cccgcgctcc tgtaactttt gctgatgctg
     1381 cacccttttt gcagcaggca ctcgacaacg gctggctgac aaccggcccg ctggtgcagc
     1441 agtttactgc gcaggttgcg gagtttctgg gtaccagcac gctgattccc gtctcttcat
     1501 gcaccgccgc actgcagatc gtattggccg cagctgactt ttctcccggt gacgaagtca
     1561 ttgtcccggc aaatacgttc attgccacct ttgaaaccgt ggagctggcc aacctgaaac
     1621 cagtactctg cgatattgat gcagaaacct ggaacctgga tattcatgct gccgcgaagc
     1681 tgatcaatcc gcgcacacgc gccatcatcg ctgttccgtt tgcgggcaat ccgctgccga
     1741 tggaaccgct gcgggccctg tgcgacaagc accagctgcg gttaatcctg gacaatgctc
     1801 atgcgctcga aacgaagtgg aacggaaaga acctgcacca ctacgccgat tacagctgtt
     1861 acagctttta tgcaacgaaa aacctgacca ctgctgaagg cggagcaatc gtctgccgcg
     1921 aagcagaagc cgagcgcatc agggcactca gcctgcatgg tatgtcccgc gccgcatgga
     1981 accgctatgc cggcggaaca tggcgctacg atatcgtcga aacgggtttc aaggcaaacc
     2041 tgacggatat tcatgcagcc atcggcctgg ctcagctgcc gaaattagct gagcaccacg
     2101 agcgccgccg ttatcttgcc ctgcgttacc gcaatgcact aaaagatctg cctgtccgtg
     2161 tgcagcagat cgacgaggga ggcagcgaca cagatcatgc gtggcacctc ttctgcattt
     2221 ctgtcaggcc tgaagcctcg gtcacgcgcg aaaacctgat ggaagcgctg gcagcagcgg
     2281 gtatcggcta ttcagtccac tttattccgg tctttgagtt cagcaacaca cagcgaaaat
     2341 acaactttcc ttcgggggag tttcctgcga ccacgggcta tttcaccggt gcgctgtctc
     2401 tgccgctgta tccgacgctg gcagacgccg atcaggacga ggttatccgt gttcttcgtg
     2461 ctgcttttcc aggtacttag gcagcgtcgg cgcagcgatg actgtgcggt cttcacccac
     2521 gtagatcgcc tgcttgccct gcacagcccg cattccgctg cggaagagca tacccttacc
     2581 aaagtttatc tcgacattgg gataaagctg gttttccaca agcacgtacg ccctgtcgtg
     2641 cgcgaggtat gagttctgcg ccgattcaag ctgccgctgg tctgactcaa ccagcgcctt
     2701 gtacttgcgc atggcaagca acagtttttc gcgtgtttcg gcctctttgg catcgagctt
     2761 ccgctttttc atggcatcgt tcaaccggtt caggctgacg cgtatttttt ccagcgtaac
     2821 ttccttttct ttcagcgaag cgtgaatttc gtcaatcgcg ctgcgcatag ttgggtcaat
     2881 accgactgtc aggaccgtac gcgtttcgcc ggcccctccg agcttgttgc agtgaacaaa
     2941 tttgccagcg gagatatcgc cgccggtaat gtcaccccgg ccccccttca caacaatcga
     3001 accgccggca acgaggtgcg aatgcagcgt cgcctcagag atgaagatag atcccttggc
     3061 gataatagtg ccctgctcga cgaaccgcgc gtagacgtcg ccatcagagg tgattttgcc
     3121 ttcaccacga cccatgaagc ctgcagacag aatgatatct ttcccggcgg taatttcaca
     3181 gactccgaca ctcgactgca gaataatgct gcctgaggca tgcagcttga acccgtctgc
     3241 gaccgattct tctacaatta ctgagcccgg aaaatccacg ttgccggttg agtagtcgat
     3301 gttcttgagg tagaccactt catcgacgcg gatcttgcca ttgctttcaa gaaccggcct
     3361 gccttctatt tttgatatga gcttcttgcc atccggcgaa atttccacgt ttgcgccggg
     3421 aataaattcc gcaatcacac ccggttcggc cggaagaagt ttacctccca ccgtgaaacc
     3481 tgtttcaccg acggtaggat caattttttc agcaattact tcaccggcct tggcagattt
     3541 gatcaggtta acctgtttgt ggtcgatctt ctgcgccttc gggtcgacgc gcggccgtgt
     3601 cattgtttca aaaagcggct gtataaaccc attcttgccg cgcaccggtt ctttgccctg
     3661 tgccacgaga atcatccgct tgtacgatgg ctttactccc agttcgcgca acgtgtcatc
     3721 gagaaggcct ttgatgacgc catgctcgcg caacagtgcc ctaaggcgtt cttcgggaat
     3781 tgccttgccg ccaccttcag gaggctgcag gaaaaggtgt gcgctcatgt ggtcgtcaga
     3841 aacttcaact tcggccactg cgtctttgcg ctgggcgact tttggccagt cgccgataac
     3901 gtgtgcttcg ccctcggcga ttttgccctc ttgcgtagaa cgcagcagcc tctcaaccga
     3961 aacatttttg tccggcacga tgtgcattga gcgcaggcga ttattgaggt ctgtcgggtg
     4021 aacagcacgc ccctttccag tcgggttaaa gatgcgcagc acggccttgc ctttctcttc
     4081 tgtgagttca aagctgccgt tctgcgatga ctcgagctct tcgaggagct ttcgggtcat
     4141 ctcattttca ggcggtgggc acattcaatg ccatcggaaa atcttcaggc tgattgaaaa
     4201 gcaaaagacg ccaaacgcat tgagaatggc tgcggggatg agaatctcgc gccattcgcc
     4261 cgaggaattg aagactgtgc ggagcgcgtc ggcgagcaat gccaggggcg aatactgcag
     4321 caagggctgt atggcctcgg gaaaattggt gtagctaaag aagatacccg atgcgagcat
     4381 aagcggtatc gaaaagacgt tgatgacgcc gtttcccgta cgggtatttt cggcccgtga
     4441 tgccagcaga aacgccagcc ctgcaaatgc aatattcccg gaaatgaaga gcagcagaat
     4501 actgccaata tcgccctgca gctggaagcc aaaatagaga taaccaaaca ggaacaggag
     4561 aagcgcctcg acaaagttaa tcgtcatgcg tgacaggaaa aacgaagcaa gaattgccgc
     4621 acggctgacc ggcgtcgcaa tcatgcgtcg catgagtttt ttcatccggt actcaatcag
     4681 cacatagccg attccccaga ggcaggagtt catgataccg agtgcgatca gtcctggaat
     4741 aagaaagtcg atataccgac tgccatgggt ggtgacgggc gttagttgca tctcgaccct
     4801 ttcctggctt agccgccggg atagaatgag gtatgagagg tacgaagtct cgtttgccgg
     4861 atcgagatgg aagcggacca ctccttcacc cgagagttct atgaacagga cgaccttgcc
     4921 cttgcggagc agcctgagcg cattctcctg gtcggtaaaa gagaagcgga agacgctgtg
     4981 atcgttcgtc tttttgggcc gtgaaatatt gcggttttcg atttcgttga tcacttcacg
     5041 cagttcagag ctctgccgca ggtcaccaac gactgctatc tgccgcgtgg tcactttctt
     5101 ctggacaaag gcgataccca ggacccagga cattgccacg ggtagcagca ccgaccagag
     5161 tattatctcc cagtcgcgaa aaaattcgcg caggtgcatc aggtacaact ggcgcagcgc
     5221 gctaaccatc gaggtgcctg cctgtcatct tgataaaaag gtcgtcgagc gtcaaaggcc
     5281 gcgcggcaaa cgactcaagg ttctgccggc ccgcgcggcg cataaaatcg ggcagcaccg
     5341 tggctgcgtc gtcgacgtgc atcacaccac tgccggtctg ttcgtcaata ttgaaatatg
     5401 aaaccttctt catctgccgc agccccgcga tttggcgggc ctgtactttc ttgagtcgaa
     5461 actcgataat ttcatggctg gcatgtgctt cgagcaggtc ttccagcgcg ccgtctgcca
     5521 agatacgacc tctgaactgt atgacaatac gctcacagag ctcttcggcc tcttccatat
     5581 agtgggtcgt cagcagcagg gtcattcccg cgcgtttgag atcagacaga atctgccaaa
     5641 gctcgcgccg cgcgtgtggg tcaagccccg tcgtcggttc gtcgagcagc aacacctttg
     5701 gtttctggat gattgcaata ccgagcgcca gtctttgccg ctggccgccg gagagtgtat
     5761 tcacgtaagc atttttcttt tcggccagcg aaaccaggtc gagaatttcg accgtgcgcg
     5821 caggcgaagc tccataaaat gccgcgaaca ggcgcagcgc ttcggcaacg cgtatctttt
     5881 ccggaaaacg cgtctcctgg aatgcaaggc ccatgatctg ccgtatttgc cgttcatgct
     5941 gtgcgtagtt cagtccgcaa atttcgatgc tgcccgagtc gggtgtctgc agtccctcga
     6001 tcatttctag cagtgtggtc ttgcccgcgc cgttgggccc tagtaacgct acgaactccc
     6061 cctctttgat ttcaagagag agatcattca ccgcgtgcac gccgccaaaa ctcttgcgga
     6121 cgttacgaat cgataaaact gtctgtcggc ccgcgcgtgg caactcagct acctacctcg
     6181 cgtagctgcg gcagtgggcg accaatttcg ttacccagct ccgctgtgct gacggtggct
     6241 gttcccagct tacccacaac gatgccgccg gccaggttag aaagtaccat tgcttcggtg
     6301 tgctccgctc ccgcacaaat cgcggctgta taaacgctga tcacggtatc accggcaccg
     6361 gtgacatcga acacttcgcg tgccaccgtg ggcagatacg cagatttttt gtcggtgaag
     6421 tatgccatac cacgctgtga tcgggtgatc agcaggtgcg gtaggcgcag cgtttcacga
     6481 attttcgtgg caatcgtgtg tacttcttcg tcgctctgcg ggaaaggcat gccgatacct
     6541 tctgaggcct ctttctcatt cggcgtcatg atgtcgcatt cgagatactg cttgaaatgc
     6601 ttcacctgcg gatcaacggc gacgaagacc ttacgcttat gcgcaatctt tatcgcttcg
//
LOCUS       DAOQJF010000175         6630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6461_length_6630_cov_5.268441, whole genome shotgun sequence.
ACCESSION   DAOQJF010000175 DAOQJF010000000
VERSION     DAOQJF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_6630_cov_5.268441"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PLF85_10035"
     CDS             <1..910
                     /locus_tag="PLF85_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE20049.1"
                     /translation="EAIAVEDHEYTGADKKCGNCGTANAAKAEFCTQCGSPMKDAKTV
                     SLRSEQATAEGTAFAADSKADAKKDFEAQKAGLTGAAAAGGEEPPTSNKKWYIIGGVA
                     GAAVVALIAGFFMSRPVEVKVESHNWERVVKVESYKGRSESAWCSSMPYDAYMVIRKR
                     EVSSTNQVADGETCDTVRVDKGNGTFKKERRCKTKYKSVPVYSDKCYYRVNRWGHERD
                     LIAKGGLNDQVAWPAVNIRAGTCLGCEREAGRSESFNLVLAQVKKPDKKHNCSYPESK
                     WRAIPDGAVKKIKVRMVGGAKCDTLE"
     gene            945..2090
                     /locus_tag="PLF85_10040"
     CDS             945..2090
                     /locus_tag="PLF85_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20050.1"
                     /translation="MKSTQPWIYSPFIDGLFILGVPFMVVAAVFAFPATFTPPDSMTV
                     VSWAILVMGVDVSHVYSTLYRTYFDAETRQRHAALLYLLPFAVLVISMLVYAAGAMIF
                     WRVMAYLAVFHFARQQYGFMRVYSRNENESPVTRRMDALAIYTATFYPILAWHLQGQK
                     AFNWFMKGDFAYFTSATAGDIARFFYIAVIALYTAKELILLWTSFAAQKKLPLPGNSK
                     AGLPGTYQFNLPRNLIIAGTFISWYVGIVHFNSDLAFTAINVMSHGIPYMALVWIWGN
                     KRYCAESSTRHNIFQPRYVALFAGLLLTLAYIEEGFWDVWVWHEPEHAAFFSIFHDTL
                     GDLDNGSPWLRLVVPLLSVPQVTHYIIDGFIWKVRNDRYGWRKAVLD"
     gene            2202..2945
                     /locus_tag="PLF85_10045"
     CDS             2202..2945
                     /locus_tag="PLF85_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNE20051.1"
                     /translation="MGELMEGSGKKLLLVTAAAVTFVGATLIAQDAANVKGQKKADVQ
                     DVLLNDFESSEDWRAFSTTPLGITKVRKTIQLGPIEDVFNPKELSEEEKTRFKDGLAD
                     PKTANNVLGVKTFFKEKGFDRVEIKPPHEYLIKGLGRQFSVWVLGRNFRHTLFIKLRD
                     YKGNLHKLKLGKLDYFGWRKHTVTVPGWLPQSTRYALLDKNLHFVSLYVECDPHETWG
                     DFYFYIDDLRVKTDMSDSEYPGSKIKDNW"
     gene            2963..3853
                     /locus_tag="PLF85_10050"
     CDS             2963..3853
                     /locus_tag="PLF85_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNE20052.1"
                     /translation="MHNAIKYSVLAVIPLLYSFNALTGASAVKTLNGDDVDGSELRSV
                     QVEGFEADTWSAVVSPGDPEAIPAVVDDKGSPMQGEKWNSPEVAIKGEANKPCRPQNL
                     AYDETNKKCLGVRFKFIYPGNNVVALIPPNSKKVYRVLDELDHNNKPKVQEINGLKLP
                     GKVKAVSVWVLGRGNDYTLEAWVQDWKGSTHVLKMGSVNFVGWRPLTAKVPANVPQDI
                     DTYPQTKNLILTKLVLRSKPAANTEKVVVFFDAVKVLSDMYDVHFDGADVDFDDMDKK
                     EKNETREYAKQLKKYSEGQK"
     gene            3904..4881
                     /locus_tag="PLF85_10055"
     CDS             3904..4881
                     /locus_tag="PLF85_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNE20053.1"
                     /translation="MALAELRNIDSQELKRRALQLREVALGLGSRIRYSRTWWYSGAA
                     VLLAFSLARVGHSVALYLVAKGTPLPPPRAAAQQSTSSEQLFSDPTITVGGALFQRAG
                     EGAAAPGAAAVVEQPAKPFKLTATLEGNAEFARALIEVQGEGIREYCVASNFCRRKEC
                     ECSVQSATIISIAQEHIWIKMNQNRYKLKIGQSTTDLAQQAAAQPAAGPQAGPTQGTV
                     ISKVISREEVNKNILGNPAKIYEGAQFGPHLVNGKIEGYKIARVNEDHVFAKLGAKSG
                     DIIRKVNGYGLNDTERMLDLWKAIRTAPEVKIELERDGKVITYDFQIRN"
     gene            complement(4894..5694)
                     /gene="thiD"
                     /locus_tag="PLF85_10060"
     CDS             complement(4894..5694)
                     /gene="thiD"
                     /locus_tag="PLF85_10060"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNE20054.1"
                     /translation="MIPIAMTIAGSDSGGGAGIQADLKTFSALGVFGTSAITAITAQN
                     TVGVDAVEVLSSEIVAAQIKSVMTDMTVAAAKTGMLANAAIIDAVTRSVKDHQLARLV
                     VDTVMVSKSGHRLLAPEAEAAMRSVLLPAAYVITPNIPEAEVLAGIKIASYEEMKSAA
                     LKLCSLGAANVLMKGGHLNGTESPDLLFDGKDFLEFTAQRVQTNSTHGTGCTLSAAIT
                     AYLALGENLAEACRKAKQYLTGALQHAQPIGHGHGPVNHFWASSPHRG"
     gene            complement(5697..>6630)
                     /locus_tag="PLF85_10065"
     CDS             complement(5697..>6630)
                     /locus_tag="PLF85_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNE20055.1"
                     /translation="VSDPSLEEGLLGLAFAPDFARSGRYYVYYSAASPRRTIISRITV
                     GAPTQPGKWAEEELLAIRQPYSNHNGGMLEFGPDGYLYLGVGDGGSGGDPRGYAQNLR
                     SHLGKILRLDVSPATGFTVPKDNPFAQSKGDAYSEKREIFAWGLRNPWRFSFAPDGRM
                     IVADVGQNDYEELSIVTKGANMGWNIMEGFHCYSPKKNCPQKNLVLPVYEYDHGVGQS
                     ILGGYVYEGTALTNYRGRYIFADSVSGRFFALDYRQKEARAEVLIQAPGLWSSFGRLR
                     NRELVVAELQSGKIYLLAPTGQVSEKITQPTGAK"
BASE COUNT         1579 a         1839 c         1816 g         1396 t
ORIGIN      
        1 agaggcaatt gcggtcgagg accacgagta tacaggcgcc gacaagaagt gcggcaactg
       61 tggtaccgcg aacgccgcca aggctgaatt ctgtacgcag tgcggttcgc cgatgaaaga
      121 tgccaagacc gtctcgctgc gcagcgaaca ggcgactgcc gaaggcaccg cgttcgccgc
      181 cgactcgaag gccgatgcga agaaagattt tgaggcccag aaagcgggcc tgaccggtgc
      241 agcagctgcc gggggcgaag aaccacctac atcaaacaag aaatggtata tcatcggtgg
      301 cgtcgccggt gcagcagtcg ttgcattgat cgccggcttc ttcatgtcgc gcccggtcga
      361 ggttaaggtc gagagccaca actgggagcg cgtagtaaaa gtagaatcgt acaagggccg
      421 gtcagaatcg gcctggtgtt catcgatgcc ctacgacgcc tacatggtaa tacgcaagcg
      481 cgaggtcagc tcaaccaacc aggtagctga tggcgagacc tgcgataccg tgcgcgtcga
      541 caaaggcaat ggcaccttca aaaaagaacg ccgctgcaaa accaagtaca agagcgttcc
      601 tgtctatagc gataaatgtt attaccgcgt gaaccggtgg ggccatgagc gcgatctgat
      661 cgcgaagggc ggcctgaacg accaggtcgc atggcctgct gtgaacatcc gcgcaggtac
      721 atgtctcggc tgcgaacgcg aggctggccg cagtgaatcg ttcaacctgg tgctggcaca
      781 ggtcaaaaaa ccagacaaga agcataactg ctcataccct gaatcaaagt ggcgcgcaat
      841 tcccgacggc gccgtgaaga agatcaaggt gcgtatggta ggcggagcga agtgcgacac
      901 actggaataa tttttgattt gggcgcctga cgcccgaatc aaagatgaaa tcgacacaac
      961 cgtggatcta cagcccgttc attgacgggc tttttatttt aggcgtgcca tttatggtcg
     1021 tcgcggcggt cttcgcgttt cctgcaacat tcactccacc tgactcgatg actgtggtga
     1081 gctgggcgat tcttgtcatg ggtgtcgacg tctcgcacgt ctacagtaca ctctaccgaa
     1141 cgtattttga tgccgagacc cgccagcggc acgccgcgct gctctacctg ctgcccttcg
     1201 ccgtgctggt aatttcgatg ctcgtctacg ccgccggtgc catgatcttc tggcgcgtca
     1261 tggcctacct cgcggtgttc catttcgcgc ggcagcagta cggcttcatg cgggtctatt
     1321 cgcgtaacga aaatgaatcc cccgtaacac gtcgcatgga tgcactcgcc atctacacgg
     1381 cgacgtttta cccgatactc gcctggcacc tgcagggcca gaaagctttc aactggttca
     1441 tgaaaggaga ttttgcttat ttcacatcgg cgaccgcggg cgatatcgcg cgcttcttct
     1501 acatcgccgt gatcgcactc tacacggcga aggagcttat cttattgtgg acctcattcg
     1561 cagcacagaa gaagctcccc ctgccaggaa actcgaaagc gggattaccg ggaacatacc
     1621 aatttaacct gccgcgcaac ctcatcatcg ccggtacgtt tataagctgg tatgtcggta
     1681 ttgtgcactt caattctgat ctcgccttta ccgcgatcaa tgttatgtca catggaattc
     1741 cttacatggc gctcgtctgg atctggggca acaaaagata ttgtgccgag tcttcgactc
     1801 ggcacaatat ctttcagcca cgttatgtcg cattattcgc cggcttgttg cttactctcg
     1861 catacataga agaaggattc tgggacgtgt gggtctggca tgaaccggaa cacgctgcct
     1921 ttttcagtat ttttcacgat acgctcggcg atttggataa cggttcaccc tggttacgcc
     1981 tggtggttcc gcttctatcg gtaccccagg taacacatta cattattgac ggcttcatct
     2041 ggaaggtacg caacgaccgg tatggttggc gaaaggccgt actcgactga gcacaattaa
     2101 atttcggcgc gcgccttaaa gaaatgtgcg ctggattccg atagtagtta cagtataaaa
     2161 ggcttttcag ggcataaata aatagcccgt taggcaaagg aatgggggaa cttatggaag
     2221 gcagtggtaa gaagttactg cttgtgacgg ctgcggctgt tacattcgtc ggcgcaacac
     2281 tgatcgctca ggatgcggct aacgttaaag ggcaaaagaa ggcagatgtg caggatgttc
     2341 tgctgaacga ctttgagtcg tcagaagact ggcgcgcatt ttcgaccaca cccctgggaa
     2401 ttacgaaagt tcgtaagacg atccagcttg gtcccattga agacgtcttt aatccaaagg
     2461 aactctctga agaagagaag acccgcttta aagacggcct ggcagacccc aaaacagcca
     2521 acaacgtgct cggtgttaag accttcttca aagagaaagg ttttgaccgc gttgagatta
     2581 agcctccgca cgagtacctg atcaagggcc tcggtcgcca gttctcggtc tgggtactgg
     2641 gccgcaactt ccgccatacc ctgttcatca agctgcgcga ctacaagggc aacctgcata
     2701 agctgaaact cggtaagctc gactacttcg gctggcgcaa gcacaccgtg accgtgccag
     2761 gctggctgcc acagagcaca cgctatgcgc tgctcgataa aaacctgcac tttgtctctc
     2821 tctacgtgga gtgcgatccg cacgaaacct ggggcgactt ctatttctac atcgatgatc
     2881 tgcgcgtgaa gacagatatg agcgatagcg aatatcccgg tagcaagatc aaggataact
     2941 ggtaagatca gggaggatca ccatgcataa cgcaatcaaa tactcggtac tcgcggtaat
     3001 accgctgctg tactcattca acgctcttac aggagccagc gcggttaaga cgctgaacgg
     3061 cgacgacgtc gacggttcag agctccgctc cgtgcaggta gaaggctttg aagccgacac
     3121 ctggtcggca gtggtctcac cgggcgaccc ggaagctatt cctgcagtgg tcgatgacaa
     3181 gggttcgcct atgcagggtg aaaagtggaa ctcccccgaa gtagcaataa agggtgaagc
     3241 aaacaaaccc tgccgcccgc agaacctcgc gtacgacgag accaacaaga agtgcctcgg
     3301 cgtgcgcttc aagttcatct acccgggcaa taacgttgtc gccctgatcc cacctaactc
     3361 aaagaaggtg taccgcgtac tcgacgagct cgaccacaac aacaaaccga aagtacagga
     3421 aatcaacggc ctgaaacttc ccggtaaggt taaagctgtg tctgtgtggg ttcttggccg
     3481 cggtaacgac tacactctcg aagcctgggt acaggactgg aagggctcaa cgcatgtact
     3541 caagatgggt tctgtcaact tcgttggctg gcgcccgctg accgcaaagg tacctgccaa
     3601 tgttccgcag gatatcgaca cctacccaca gaccaaaaac ctgattctga ccaaactcgt
     3661 gctgcgctca aaaccagctg cgaataccga aaaagtcgtg gtcttcttcg acgctgtgaa
     3721 ggtattatca gacatgtacg acgtacactt tgacggtgct gacgtcgact tcgacgatat
     3781 ggacaagaaa gaaaagaacg aaacccgcga gtacgcaaaa cagctcaaaa agtactccga
     3841 aggccagaag taacagcctg aatatagtag tcgcgctgag cgcgactact atatactcct
     3901 tatatggcac ttgcggaact ccgcaacatt gattcccagg aactcaaacg gcgcgcgctg
     3961 caattgcgtg aagtcgcttt gggcctgggc tctcgcattc ggtacagccg tacatggtgg
     4021 tactcgggtg cagctgtttt gctcgctttt tcactggcgc gagttggtca ctccgtcgcg
     4081 ctatacctcg tagccaaggg aactccgctg ccgccgccac gcgctgcagc ccaacaaagc
     4141 accagcagcg agcagctgtt ttctgacccg acgattacgg taggtggagc gctgtttcaa
     4201 cgggcaggcg aaggcgctgc tgcgcccgga gccgccgcag tggtcgagca accggccaaa
     4261 ccgttcaaac taacagcaac gctcgaaggc aatgcagagt ttgcgcgcgc gctgatcgaa
     4321 gtgcagggtg aaggtatcag ggaatattgt gtggcctcaa acttctgccg gcgtaaagaa
     4381 tgcgagtgct ctgtgcaatc agcgacaatt atctcaatcg cgcaggagca tatctggatc
     4441 aagatgaacc agaaccgcta caagctgaag atcggtcaat cgaccaccga cctcgcccag
     4501 caggcagcgg cgcagccggc tgcgggaccc caggcgggac ccacgcaggg aaccgtcatc
     4561 tcgaaagtta tttcacgcga agaagtgaac aagaatattc ttggtaaccc cgcaaaaatc
     4621 tacgaaggtg cgcagtttgg cccacacctg gtaaacggca agatcgaagg ctacaagatt
     4681 gcgcgggtga acgaagacca tgtgtttgcg aagctgggtg caaagagcgg cgatatcatc
     4741 cgcaaggtga atggctacgg tctgaacgat accgagcgta tgctagacct ttggaaagcg
     4801 atccgcacgg cccccgaagt caagattgaa ctcgaacgcg acggaaaagt catcacctac
     4861 gattttcaga ttcgcaactg atctgcacgc gcctcacccc cgatgcgggg atgaggccca
     4921 gaaatgattc accgggccat ggccgtgacc tatcggctgg gcatgctgca aggcgcctgt
     4981 caggtactgt tttgctttgc ggcaggcttc agcgagattt tcaccgagcg ccagataggc
     5041 agtgatcgcc gctgaaagcg tacagccggt gccgtgcgtt gagttcgtct gtacgcgctg
     5101 ggccgtgaat tcaaggaaat ctttgccgtc aaacagcagg tcaggcgatt ctgtgccgtt
     5161 caggtggcca cccttcatca ggacatttgc tgcgcccagg ctgcagagct tcagcgctgc
     5221 cgatttcatt tcttcgtagc ttgcaatttt tatccccgca agcacctccg cctcgggaat
     5281 attcggggta atcacgtaag ccgccggcag cagcacgctg cgcatggcag cctcggcttc
     5341 aggagccagc agccggtggc cgctcttgga gaccatcacc gtatcgacca cgagacgcgc
     5401 gagttggtga tcttttaccg accgggtgac tgcatcgatg attgctgcat tggcgagcat
     5461 gcccgttttt gcggcagcga ccgtcatatc ggtcatgacg ctcttgatct gtgctgcaac
     5521 aatttcggac gaaagcactt ctacggcatc gacaccgact gtgttctggg ccgtgattgc
     5581 cgtaatggca ctggtgccga agacgcccag cgccgaaaag gttttcaggt ctgcctgtat
     5641 gcctgcgccg ccacccgagt cgctgccggc aattgtcatt gcaatgggta tcataactat
     5701 ttggctcccg ttggttgtgt gattttttca gagacctgcc ccgtcggggc aagcaggtaa
     5761 attttaccgg actgcagttc ggcaacgacc agctcgcggt tgcgtaaccg cccgaagctc
     5821 gaccacaggc ccggcgcctg aatcagcact tcggcgcgcg cctctttctg ccggtaatcc
     5881 agcgcaaaga aacgacccga gaccgaatcg gcaaagatgt agcgcccgcg gtagttcgta
     5941 agcgcggtac cttcatagac gtaaccgccc aaaatggatt ggccgacacc gtggtcgtat
     6001 tcataaacgg gcaaaaccag gtttttttgc ggacaattct ttttagggct ataacaatga
     6061 aaaccttcca tgatattcca gcccatgttc gcgccctttg tcacaatact cagctcttcg
     6121 tagtcattct gcccgacatc ggccacgatc atccggccgt cgggcgcaaa actaaagcgc
     6181 cacgggttgc gcaaccccca ggcaaagatc tcgcgcttct cgctataagc gtcgcccttg
     6241 gactgtgcga atggattgtc cttgggtacg gtaaaacctg ttgccggcga aacatcgagc
     6301 cgcagaatct taccgagatg agaacgcagg ttctgcgcat aaccccgcgg gtcgccgccc
     6361 gaaccaccgt cgccaacacc caagtagaga taaccatccg gcccgaattc gagcatgccg
     6421 ccgttgtggt tgctgtacgg ctggcgaatc gccaaaagct cctcttcggc ccacttgcct
     6481 ggctgagtgg gagctcccac agtaatacgg ctgatgatcg tgcgacgcgg tgaggccgca
     6541 gaatagtaga cgtagtagcg gccgctgcgc gcaaaatctg gtgcaaacgc gaggccgagc
     6601 agcccctctt caagactggg gtcgctgact
//
LOCUS       DAOQJF010000176         6618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6477_length_6618_cov_5.223221, whole genome shotgun sequence.
ACCESSION   DAOQJF010000176 DAOQJF010000000
VERSION     DAOQJF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6477_length_6618_cov_5.223221"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLF85_10070"
     CDS             complement(<1..347)
                     /locus_tag="PLF85_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE20056.1"
                     /translation="MHVSDDHFKYPRQYDAIVVGAGHAGCDAAHICAKAGLKTLLVTM
                     SLDTIGQMSCNPSIGGVAKGHMVREVDALGGLMGVATDRTGIHYKMLNRSKGPAVRGP
                     RAQADKKLYQNEV"
     gene            complement(417..986)
                     /locus_tag="PLF85_10075"
     CDS             complement(417..986)
                     /locus_tag="PLF85_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNE20057.1"
                     /translation="MKNKRTLILVLAGIPWLILASMVIKAYLPIFTGRSYLLPVQARD
                     PRDFFRGNYVSLRYEFSTINLDKVAHDLKPGQSYEFGDTMYLAMRESDGALTATGLYS
                     ERRPDAAIVLKVQPDSRLSGGDKHASLATGLESFFAPKTDALAWEDALRQGRVFARVS
                     IDHSGNARLTGLELKAAPKVKSGETEEQE"
     gene            complement(976..2223)
                     /locus_tag="PLF85_10080"
     CDS             complement(976..2223)
                     /locus_tag="PLF85_10080"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNE20058.1"
                     /translation="MNYHKLKAAVESWAQSGIISSAQGEKILALYEHDVPVYKTMRFW
                     LMSLAVILLGFALFLVISANWQHLHWAAQSLIALAPLVAAQFFAIRSEMRDERLNADL
                     GWFAASIALGANIMLQAQIFHISAYYPNGVLFWVIGMAPVLWWRPTTITYLLASVLFV
                     VYMVMQMEHRQFSLLSAIPLIALVRVAVIRQTAINTLALIVNFYVFAIMLMVYRDFRH
                     PGMLWEISHLALALTIVHRLHQLKESNFSLLLLLLSLGFHLMALIATFKSGARWLAGR
                     ENAWLPVLVAGVSIVALYRMRATLREKRLTTILLGGITALFTVGLISGAVKSAQNGDS
                     QLLARIGANVVYLGSLVALIFQAIAVRAKRLFLASAFGLLLWTWIRYLDLFQDYLVTA
                     LIFAVSAVALIGLNKMWESKYEK"
     gene            2391..3755
                     /locus_tag="PLF85_10085"
     CDS             2391..3755
                     /locus_tag="PLF85_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE20059.1"
                     /translation="MQGSEDPVRQGSGDFVGFGGFERAMLNSAGVAIVATLPDGIITH
                     FNPEAERMLGYRARELIGRYTPEIFHDRAEIELRARELSSEPGHEVKPAFDVFVQNTR
                     AEKAESREWTYVRKDGSRFSVWLKVTPILDDDGAIEGYLGVARDITELKQALQAAERA
                     NRAKSEFLSRMSHELRTPLHAIIGFTRLLSADSVLDPHERETYLSHVGNAGVHLLNLI
                     NEVLDLSAIESGKMSYRITGFSTARILPDVASYLMPLLRQKRIQLEIIPSECIVRADY
                     LRLKQSLLNLVSNAVKYSEPGGVILLTCQPASAETIRLLITDTGKGIPEAMAQKLFTP
                     FERLGADENTEGAGIGLSITRKLIEGMGGSVGHFNNHDQGSTFWIELPLGTEESSPVD
                     RPKGSEGLNSISAEAAATLRILLVEDNPVNAALMQAWFRRHPGYELVVAGNAAEALGV
                     LEKT"
     gene            3752..4066
                     /locus_tag="PLF85_10090"
     CDS             3752..4066
                     /locus_tag="PLF85_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE20060.1"
                     /translation="MIDIAFIDVHLPDASGFDLLRQVRSLERTRFMPVIAISADAMDY
                     QVQAALNAGFDEYLTKPLDFDLLQLSLARWRSRLRRLESTAVAGRKICVTCTTVCSDF
                     ND"
     gene            complement(3998..4858)
                     /locus_tag="PLF85_10095"
     CDS             complement(3998..4858)
                     /locus_tag="PLF85_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20061.1"
                     /translation="MPATTADHITAFIQALLVARTNTAPLFIGLQGPQGSGKTTACNA
                     AVARLQAEHNLRGVTLSIDDFYLTRAEQVKLAAQHAGNVYLSQRGYPGTHDIPLGQKI
                     LAQLRTINQQKKPVAVPRYDKSLHGGEGDRLPESAWPVIDSPLDFVLLEGWCMGFTPL
                     PEAALPNAQLAEVNRLLAQYSGWYDFLGAFIQLRTADITHAVGWRIEAEEKMRSAGKS
                     AMSAEKIRAYIERFLPAYELYLPELARNISAAERPVADSGSFFRSRLIIEIATDRSAR
                     HADFSTGNRG"
     gene            5661..>6618
                     /locus_tag="PLF85_10100"
     CDS             5661..>6618
                     /locus_tag="PLF85_10100"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNE20062.1"
                     /translation="MCDTLVATPDYTKSRTMILAKNSDREPNEAQSVVRYPRTRQKQK
                     GLKTTFIQIPQVKETYEVILSKPFQMWGAEMGVNEHGVAIGNEAVFTKITPPKKNDGL
                     TGMDMLRLALERSKSAAAALECITELLAEFGQDACGGYENKDMFYFNSYIIADAKEAW
                     VLETVDRHWVAEKVKGFRSISNGLTIETDFDLASEGLKDFAQQQGLTRGKDFNFRKVF
                     SDRFYTYFSHCKVRQALSTELGRKGARGYDVQTAMNILRSHHADKTDPARTGMHSLCM
                     HAAGMTTPSQTTGSMVAELRPAGKSTYWFTGTAAPCLSLFKPL"
BASE COUNT         1439 a         1818 c         1961 g         1400 t
ORIGIN      
        1 ttcacttcat tctggtagag tttcttgtcg gcttgcgcgc gcgggccgcg cacggccggg
       61 cccttgctgc ggttgagcat cttgtagtgt atgcccgtgc ggtcggtcgc cacgcccatg
      121 agaccaccga gcgcatcgac ttcacgcacc atgtgtcctt tcgcgacgcc gccaatcgag
      181 ggattgcagc tcatctgccc gatggtgtcg agcgacatcg ttacaagcag tgtcttgagc
      241 cccgccttcg cgcaaatgtg cgcagcgtcg cagccggcat gtcccgcgcc gacgacaatc
      301 gcgtcgtatt ggcgggggta tttgaagtgg tcgtcagaga cgtgcacggg tcagtacagg
      361 tggtttagac aggtcgtaaa gcggtgtcag gtttggcctg ccccaggcga ctctgcctat
      421 tcctgttctt cggtttctcc gctcttcacc tttggtgcgg ctttgagctc taagcctgtc
      481 agtcgcgcgt tgcccgagtg atcgatcgag acccttgcaa agacgcgacc ctgcctgagc
      541 gcgtcttccc aggcaagtgc atcggttttg ggagcgaaaa aagattctag gccggtagcg
      601 aggcttgcgt gcttgtcgcc accgctgagg cgcgaatcag gttgcacttt gagaacaatt
      661 gcggcgtcag gacggcgttc agaatagagg cccgtagcag tgagagctcc gtctgattcg
      721 cgcatggcca ggtacatggt gtcgccgaat tcataacttt ggccgggttt aaggtcgtgc
      781 gcgactttgt cgagattgat cgtcgagaac tcgtagcgta gcgatacata attgccgcga
      841 aagaagtcgc gcgggtcgcg tgcctgcact ggcaaaagat aagagcggcc cgtgaagatc
      901 ggcaggtatg ccttgatgac catcgaggca agtatcagcc acggaattcc cgcgagcaca
      961 agaatgagtg tgcgcttatt tttcatattt gctctcccac attttgttca gaccgatcag
     1021 cgcaacagcc gacacggcaa aaatcagcgc cgtgacgagg tagtcctgga acaggtcgag
     1081 atagcgtatc catgtccaga gcagcaggcc gaaagccgag gcgagaaaca ggcgcttggc
     1141 gcgcacggcg attgcctgga aaatcagtgc gacgaggctg cccaggtaga cgacattggc
     1201 tccaatgcgc gcgagcagtt ggctgtcgcc gttctgcgcg ctcttgacag cccccgaaat
     1261 tagtcccact gtaaaaagcg ccgttatgcc gccgagcagg attgtcgtga gtcgtttttc
     1321 gcgcagcgtc gcgcgcatgc ggtacagcgc cactatagag acgccggcta ccagtaccgg
     1381 caaccaggca ttctcgcggc cggcgagcca acgcgcacct gatttaaagg tcgcgatcag
     1441 cgccatgaga tgaaaaccga gcgagagcaa tagcaacaac agcgaaaaat tgctctcctt
     1501 gagctggtgc agtctgtgca cgatggtaag cgcgagagca aggtgagaaa tctcccacag
     1561 catacccggg tggcgaaaat cgcggtagac catgagcatg atcgcgaaga cgtaaaagtt
     1621 cacgatgagt gccagggtgt tgatcgcagt ctgcctgatg acggcgacgc ggaccagtgc
     1681 gataagcgga attgccgaca gtagcgagaa ctgcctgtgt tccatctgca ttaccatgta
     1741 gacaacgaag agcacgcttg cgagcaggta cgtgatggtg gtcggtcgcc accacaacac
     1801 gggagccatg ccgatgaccc agaagagcac gccgttcggg taatacgcgc tgatatgaaa
     1861 aatctgcgcc tgcagcatga tgttggcgcc cagtgcaatc gaagccgcga accagccaag
     1921 atctgcattg agccgttcat ctcgcatttc gctacgaatc gcgaaaaatt gcgctgcgac
     1981 cagcggggct agcgcaatca gcgactgtgc cgcccagtgc aggtgctgcc agttggccga
     2041 gatgacgaga aacagagcaa agccgagcag aatgacggcc aggctcatca gccagaagcg
     2101 catcgtctta tacacgggaa cgtcgtgctc atagagcgcg agaatctttt caccctgcgc
     2161 actgctgatg atgccgctct gcgcccacga ttcgacggcg gccttgagtt tgtggtagtt
     2221 catattgttt gcctgttggg atgcaatttg acctaccagt ggcaggccgc ctcgcgatgt
     2281 aaagcccctt cgcctcaatg agaaaagtta tcagactatg tctaaaaatg aataaaagac
     2341 cctaaattcg tttattgttg ccgtattcac gctccttatg gccgtggcgc atgcagggct
     2401 ccgaagatcc ggtgcgccag ggcagcggcg actttgtcgg gtttggcggt ttcgaacgtg
     2461 cgatgcttaa cagcgcgggc gtagcgattg tcgccaccct gcccgacggt atcataacgc
     2521 acttcaaccc cgaagcggag cgcatgctgg gttaccgcgc ccgcgaactg atcggtcggt
     2581 ataccccgga aattttccat gaccgggctg aaatcgagct gcgcgcccgc gagctttctt
     2641 ctgaaccagg tcatgaagtc aaaccggctt tcgatgtgtt tgtgcagaac acccgcgctg
     2701 agaaggcaga gtcgcgcgag tggacgtatg tccgcaaaga cggctcacgg ttttctgtct
     2761 ggctgaaggt gacgccgatt ctcgacgatg atggcgcgat cgaaggttac ctgggtgtcg
     2821 cacgcgacat caccgaactt aagcaggcgc tgcaggcagc cgaaagagct aaccgcgcca
     2881 agtcggagtt tctttcgcgc atgagccacg aactgcgcac gccgttgcat gcgatcattg
     2941 gcttcacccg tctgctgtct gccgatagtg tactggatcc gcacgagcgg gaaacgtacc
     3001 tttcgcatgt cggcaacgcg ggtgtgcacc tgctcaacct gatcaacgaa gtgctcgatc
     3061 tcagcgcaat cgaatccgga aagatgagtt accgcatcac cggcttcagc accgcgcgca
     3121 tactgcccga tgtcgcgtct tacctgatgc cgctgttgcg gcagaagcgc atccagctcg
     3181 aaattatccc cagcgaatgt atcgtgcgcg ccgattacct gcggttgaag cagagcctgc
     3241 taaaccttgt atccaacgcg gtgaagtaca gtgaaccagg gggagttatt cttctgacgt
     3301 gccaacctgc gtcagcagag acgatccgcc tgctgattac cgacaccggc aagggaatac
     3361 ccgaagcgat ggcgcagaag ttgttcacgc cctttgaaag acttggcgcc gacgaaaaca
     3421 cggaaggcgc gggtatcggc ctgagtatca cccgcaaact cattgaagga atgggtggta
     3481 gtgtcggcca cttcaataac cacgaccagg gttcaacttt ctggatcgaa ctgcctctcg
     3541 gtactgaaga aagttcaccg gttgaccgac cgaagggtag tgagggcctc aacagcattt
     3601 ctgcagaagc ggcagcaaca ctgcgcatac tgttggtaga agataacccg gttaatgctg
     3661 ctctgatgca ggcatggttt cgccgtcacc cggggtacga gctggtggtg gcaggcaatg
     3721 cggccgaagc actgggtgtg ctggaaaaaa catgatcgat atcgcgttca ttgacgtcca
     3781 ccttcctgac gccagcggtt ttgatctgct gcggcaggtt cggtcgcttg agcggacgcg
     3841 tttcatgccc gtcatagcaa tcagcgccga tgcgatggat taccaggtgc aggcggcgct
     3901 gaatgccggg ttcgatgagt acctcaccaa gccgctcgat ttcgatctgc tgcagttgtc
     3961 actggcgcgc tggcgctcgc gcctgcgccg cctcgaatca accgcggttg ccggtagaaa
     4021 aatctgcgtg acgtgcacta cggtctgtag cgatttcaat gattaatcgg ctcctgaaaa
     4081 aagagccgga atctgctaca ggccgttcag ccgctgaaat attccgcgca agttccggca
     4141 ggtagagttc gtaagccggc agaaagcgtt cgatatatgc cctgattttt tccgcgctca
     4201 tcgcgctttt tccagccgag cgcatttttt cttcagcttc gatgcgccag ccaacggcat
     4261 gcgtgatgtc agcggtgcgt agttgtatga acgccccgag aaaatcatac cagccgctat
     4321 attgagccag cagacggtta acttcggcaa gttgtgcatt gggtaatgcc gcttcgggta
     4381 acggggtaaa tcccatacac cagccctcga gcaggacaaa gtcgagtggt gagtcgatca
     4441 ccggccaggc cgattcaggc agcctgtcgc cttcgccgcc atgcagagac ttgtcgtagc
     4501 gcggtacggc gaccggcttc ttctgctggt tgatggtgcg tagctgtgcc agtatcttct
     4561 ggcccagtgg gatgtcatgc gtacccgggt agccgcgctg cgagaggtac acgttacccg
     4621 cgtgctgtgc tgcgagctta acctgttcag cgcgcgtgag atagaagtcg tcgatcgaaa
     4681 gcgtcacgcc gcgcaggttg tgctctgcct gcaggcgtgc gaccgccgca ttacaggcgg
     4741 tcgttttacc tgaaccctgc ggaccctgca ggccgatgaa gaggggcgcg gtgttcgtgc
     4801 gggcgacaag cagcgcttgg atgaacgcgg tgatgtggtc agcggtggtt gcaggcattt
     4861 ctgtttttca cctcaggtca tcgttcaacc agtcttgcgg attttgtctg atataggtcc
     4921 gtatttggct gagtgaattc tcatcgcgga tgatgtgatc atagaatcgt ggctgccagg
     4981 cgaaattacc gaggccagat tttctgattt cgaaggttga gcgtcctttg aaccagcgga
     5041 taatctggga caaagatgcc cctgcggcca gcgaatcgtc tgtgccggca gggacgtcac
     5101 ttaacgggga tgcgcaccga tggcgtgcgt ccccggaaat gcgatttgac ggggatgatc
     5161 gcagaccctg ttcatttgtg gggacgcgat cttgcgttgg taatcgcggt tcctgttcat
     5221 tcgtagagac gcgattaatc gcgtctctac caccgggcgg taagattgca cggggttcat
     5281 ctgcagatat ccgactattc gcgtctctac catcgggtgg taagagccaa attatgccat
     5341 gcacgtggtt cggcatgatg atatattcgg ccaattctac gtatggaaac tgctcaggta
     5401 acctatccca gaggttcgca gcgatagcgc cagcttctgc gtattgcacc tggttatttt
     5461 cgatgttacc aaaaaaatac tgccgatgct tggtgcaaat cgtgacgaaa tacgcgcccc
     5521 gggtcgcgta atcatagcct ggcagtcgtg ctgattcgac ccgatactgg ccgcggaata
     5581 gagccactgc cgggatttca gctaccgctc ccgttcgtca accgaattga ccgcgcacgg
     5641 gcgcgaacca cggctacccc atgtgcgaca ctctggtagc gacacccgac tacacgaaaa
     5701 gccgaacgat gatactcgcg aagaattcgg accgcgagcc gaacgaagcg cagagcgtgg
     5761 tgcgttaccc gcgcacacgg caaaagcaga agggtctcaa gacgacgttc atccagattc
     5821 cgcaggtgaa agagacgtac gaggtgattc tttcgaagcc gttccagatg tggggcgccg
     5881 agatgggtgt gaatgagcac ggtgttgcga tcggtaacga ggccgtgttc acgaagatca
     5941 ctccgccgaa gaagaacgat ggcctcacgg gaatggacat gctgcgcctc gcactcgaac
     6001 gcagcaagtc tgcggcggcg gcgctcgagt gcatcactga actgttggcg gagttcgggc
     6061 aggacgcctg cggtggctac gagaacaagg acatgttcta ttttaacagc tacatcatcg
     6121 ccgatgccaa agaggcatgg gtactcgaga ctgtcgaccg gcactgggtg gcagagaagg
     6181 tgaagggatt tcgttcgatc tcgaacgggc tcacgattga aacagacttt gatctggcaa
     6241 gcgagggcct caaggacttc gcgcagcagc agggcctgac gcgcggcaaa gactttaact
     6301 ttcgcaaagt tttctctgat cgtttctaca cgtatttctc acactgcaag gtgcggcagg
     6361 cgctctcgac agaactcggc cgcaaaggtg ccaggggtta cgatgtgcag accgcgatga
     6421 atattctgcg ctcacaccac gccgacaaga ccgaccccgc gcgcacgggt atgcactcgc
     6481 tctgcatgca cgccgccggc atgacaacac cgagccagac aaccggcagc atggtggcgg
     6541 agctcaggcc tgctggcaaa tcgacgtatt ggttcaccgg caccgctgcg ccctgcctgt
     6601 cgctcttcaa gccgctct
//
LOCUS       DAOQJF010000177         6615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6483_length_6615_cov_5.517073, whole genome shotgun sequence.
ACCESSION   DAOQJF010000177 DAOQJF010000000
VERSION     DAOQJF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6483_length_6615_cov_5.517073"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="PLF85_10105"
     CDS             <1..1548
                     /locus_tag="PLF85_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE20063.1"
                     /translation="PPQLRALAVRNPSFPITMRRGLNTFTLRIESPGSRLVLPLEVHD
                     EAQHITNEILDHWLLGSFFGFIACLAVVSVVFGGFSRDPIFFIYAFGLLANLGLIFIQ
                     FGLPGVVFGFAGSSLNTLTVLLTAVAAMFFTEFHRQYLRITQGRGALILRGVCAAGGA
                     VAVLAFILPFPRAVQLSLYFVILVDLVLLYFLIIQLWRREFRYSGFYLAGWVIFLGSV
                     MLHLLTIFGILPAHNVMRSAFHLSFLVQSLLFTLALGSKHRELLEEEKNTRIAMKLIE
                     QEYRQASDIHRRLLPEKKPEIPRLGVDILYIPMGQLGGDFYTFQEQDAGSWIFLIADV
                     QGHGLPAALDASSVKLAFQEAVSKKGEPGSILSEMNRLLALHLRNRFISAQVLRWLPE
                     HNKMQIASAGHPYPLLYSRASGKSRELRAEGSILGLYSEANYREFEVQLAKGDYLLAY
                     TDGLIEDPASESSYDDDMQNLMAVMESMDSDRPAAAHFARQMPRFRVSKPWSDDVTAL
                     LLTCNRA"
     gene            complement(1585..2619)
                     /locus_tag="PLF85_10110"
     CDS             complement(1585..2619)
                     /locus_tag="PLF85_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20064.1"
                     /translation="MKSRLAVLALLAATGSVLPFVINCKNPVRALERKTILKRMWSLT
                     GLKGVYTETGISATPQQTELFWRAEPPTVFARVISPGSAKGNVLHYSAGTLSLYFPKS
                     SFGIRYLKVPEISAAQQMRWLETEYDWHVAHYDIAQIDDGAVAGYPTTGVRYVPNAAF
                     SPSPFSHEWRAAVYAEFAFALETAAYRDGTEKYRLRFDRAEFMPTLTDTDFAFRFPAG
                     TTVGEYNLAGRDLSIAEARSGANFPLGIPSTTSTGFTLRRVVRAKGLIPAYTLYFEDL
                     PYQTFYTQVKDYGLKLVPEQGLKLVGKRDYRVQFAGSFRSVWFREKGVYHTVVSSRPL
                     SEVLEWLERQ"
     gene            complement(2616..3503)
                     /locus_tag="PLF85_10115"
     CDS             complement(2616..3503)
                     /locus_tag="PLF85_10115"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE20065.1"
                     /translation="MPWSQTAPLILFIVTALVTIEWLLSLLLRKKVYSFAGAMTNIAD
                     YTIHLIVAAIYGYLEYLLLVYLQLSLTITRLPVVWWYWLLLFLADDFCFYWFHRLSHG
                     LKVLWMTHVVHHSSNEFNLAVGLRQTWLPFPGMIFWLPLGFAGFRPEHILLMQAASLS
                     YQFLMHTQLINLPRFFGLLLNTPSHHRVHHGRNAECIDKNFGGVLIVWDRLFRSFAPE
                     RAAVDYGIDEIPARGEVLFVQLWGPWQYLRSLFRTGEAPTVQQDYAAQTTSPVLSAMI
                     FLAALALFFIVVRNPRWFI"
     gene            3608..3787
                     /locus_tag="PLF85_10120"
     CDS             3608..3787
                     /locus_tag="PLF85_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20066.1"
                     /translation="MKSIARDAFTFGVLLLLPGWLAAEPVITIHASGLLAARVLRYHT
                     AGQFSDDVTVMVFCP"
     gene            3874..4344
                     /locus_tag="PLF85_10125"
     CDS             3874..4344
                     /locus_tag="PLF85_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin family protein"
                     /protein_id="HNE20067.1"
                     /translation="MDEEIERLTGITRERALRIDEVNLAERLGLETIRSLSTEFYTRV
                     FNDEQQWFRNIFKQSTIEEAIQNQMEFFFQRLGGEPLYSQRKGHPALIARHMKFNMSL
                     KATERWVYHMKNALEATEAIDEDARGRMLDFFTHTAYFLHIGCTTRQRQMHQSS"
     gene            complement(4332..5348)
                     /locus_tag="PLF85_10130"
     CDS             complement(4332..5348)
                     /locus_tag="PLF85_10130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNE20068.1"
                     /translation="MADDERREFFYRLTPDEIVAALGELGLEPSGHVAQLNSMENRVF
                     DLRLENGRHVVTKFYRPQRWSKEQILEEHQFLEDLAADEVPALYPLRIGGNTLFERQG
                     IYFAVWPRTGGREPEELSTIDLQILGRLVARLHNTGAGTNMRHRPAFSAERYLVEPLY
                     ALQERKFLKQGFAAEYEDLTAHLADEWRVRSANHPHQRIHGDLHKGNMLQGSEGWFLL
                     DFDDSLSGPPLQDVWMMFSGRPEHERAVFLEAYREFRDFDDRWLADAELLRAMRIVHY
                     SGWIARRFEDPSFTAAFPDFASDAYWEEEIRILREIVQNVTGHTHEGSQPLSNKDFFF
                     DYDD"
     gene            complement(5373..5903)
                     /locus_tag="PLF85_10135"
     CDS             complement(5373..5903)
                     /locus_tag="PLF85_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20069.1"
                     /translation="MLTLILVFGACSTKKTPIPQGWFLGGQPLASTHNEILPAGATLY
                     LPEKFTLEAATDCVVRLTQQPSGETLVQLLTGTAVVQRNDPQFAVALLANDNLVRLTG
                     TRLLLQSQVGTTRIYLIDGQVMVSHAQQKWELSAKADNLFEMKAGTRAIKKIPAREIE
                     KIFPEIVRARLLMAIR"
     gene            complement(6045..6467)
                     /locus_tag="PLF85_10140"
     CDS             complement(6045..6467)
                     /locus_tag="PLF85_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HNE20070.1"
                     /translation="MNEIKRLRLQNQIFRHVALFFQKTTPSQSAKTTLADAQDKEAKI
                     TNPFELAGIEDITRQVQDYVTFTRCELSADGSFAKLFVSVWGNPHEQERLLKDIRQKV
                     PALRTSIAKNIRMRAIPQIVIVQDTSFEKASEVDKLMR"
BASE COUNT         1438 a         1781 c         1861 g         1535 t
ORIGIN      
        1 ccgccgcagc tgcgtgcatt agcagtgcga aatccgtctt ttccgataac catgcggcgg
       61 gggttgaata ccttcacact gcgtattgaa tcacccggtt cacgattggt cctcccgctg
      121 gaagtgcacg atgaggcgca gcatattacg aatgagatcc tggatcactg gctgctgggc
      181 tctttttttg ggtttattgc ctgccttgcg gttgtatccg tcgtgttcgg tggtttcagc
      241 agggatccga ttttcttcat ctacgcgttt ggactgctcg ctaacctggg cctgatcttt
      301 atccagtttg ggctgccggg cgtggtcttc ggttttgccg gtagttcgct caatacactc
      361 accgtcctgc tgactgcggt tgcggcgatg ttcttcaccg agtttcaccg gcagtacctg
      421 cgcattaccc agggaagggg agctttgatc ttgcgcggcg tctgcgccgc cggtggggca
      481 gttgccgtac tggcgttcat tctgccgttt ccgcgtgctg tgcagttgtc gctctatttt
      541 gtcattcttg ttgatctggt tctgctctat ttcttgatta tccagctctg gcgcagggag
      601 tttcggtatt cgggttttta tctggcgggt tgggtcattt tcctcggcag cgttatgctg
      661 cacctgctga cgatattcgg catcctaccc gcacacaatg tcatgagatc agcctttcac
      721 ctgagtttcc tggtccagtc gctgctcttc acgctcgcgc tgggttcaaa acaccgtgag
      781 ctgcttgaag aggaaaaaaa tacccgcatt gcgatgaagc tcatagaaca ggaatatcgg
      841 caggcgagcg acattcaccg gcggctactg cccgaaaaaa aaccagaaat tccccggctg
      901 ggtgttgaca tactgtacat acccatgggc cagctcggcg gagatttcta caccttccag
      961 gagcaggacg ccggtagctg gatatttctg attgccgatg tgcagggcca cgggctaccg
     1021 gctgcgcttg acgcctcttc agtcaagctg gcttttcagg aggctgtcag taagaaaggc
     1081 gagccgggca gcatactctc agaaatgaac cggctgctgg cgctgcacct gcgcaaccgg
     1141 tttatcagcg cacaggtctt gcggtggtta cccgaacaca acaagatgca gattgcgagc
     1201 gccggccatc cgtacccgct gctttactcc agggcatcag ggaaaagccg cgaactgcgg
     1261 gccgaaggct cgattcttgg cctttacagc gaggcaaatt accgcgaatt tgaggtgcag
     1321 ctggcaaagg gagattacct gctcgcttac acggacggtc tcattgaaga cccggcttct
     1381 gaaagttcgt atgatgacga catgcaaaac ctgatggccg tgatggaatc tatggacagc
     1441 gaccgaccag cggcagcgca tttcgccagg cagatgccgc gcttccgcgt gagcaaaccc
     1501 tggagcgatg acgttacggc gcttctgcta acctgcaata gggcttgagc tttgccggtt
     1561 gtatccgagc ttaaccgtca ggctttactg tctctccagc cattcgagta cttcgctgag
     1621 cggtcgtgat gaaacgaccg tatggtatac gcccttttcg cgaaaccaaa cggagcgaaa
     1681 gctgccggcg aactgcaccc ggtagtcgcg tttgcccacc agcttcaggc cctgctcggg
     1741 cactaacttc agaccgtaat cttttacctg tgtgtaaaat gtctggtatg gcagatcttc
     1801 gaagtacagc gtgtacgcgg gaattagccc ctttgcccgc acgacgcggc gcaacgtgaa
     1861 cccggtactt gtggtcgaag gtatgcccag tggaaagttt gcccctgatc tggcttctgc
     1921 gatagacagg tctctgcctg cgagattgta ttcaccgact gttgtaccgg caggaaaccg
     1981 gaaggcaaaa tctgtatctg tcagtgttgg catgaactcc gcgcggtcga agcgcaggcg
     2041 gtatttttcg gtgccgtcgc ggtaggccgc ggtttcgagc gcaaaggcaa attcggcata
     2101 gacggcggcc cgccactcat ggctgaaagg cgaaggagaa aacgcagcat tcggtacata
     2161 acggacgccg gttgtcgggt agcctgcaac cgcaccgtcg tcaatctgcg cgatatcgta
     2221 gtgcgctacg tgccagtcat attctgtctc gagccagcgc atctgctgcg cggcgctgat
     2281 ttccggcacc ttcagatagc gaatgccgaa gctgctcttc ggaaaataca gcgacaaagt
     2341 cccggcggag tagtgcagca cgttgccctt ggccgaaccc ggagaaatca cgcgcgcaaa
     2401 taccgtcggt ggttcagccc gccagaaaag ttcagtttgc tgcggtgtgg cggagatacc
     2461 cgtttctgtg tacacgccct tcaggcccgt cagcgaccac atacgtttga gtatcgtctt
     2521 gcgttccaat gcccgtacgg gattcttaca attgatcacg aacggcaaca cgctgccggt
     2581 tgcggcgaga agcgcgagca cggccaggcg cgatttcata taaaccacct tggatttctg
     2641 acgacgatga agaagagtgc aagtgcagcc agaaaaatca tggctgacaa aacgggagat
     2701 gttgtctgcg ctgcgtaatc ctgctgcaca gtgggagcct ctccggttct gaacaacgag
     2761 cgcaggtact gccatggccc ccagagctga acgaaaagga cttcgccccg cgcaggtatt
     2821 tcatcaatac cgtaatcgac tgccgcgcgc tccggcgcga agctgcggaa cagacggtcc
     2881 catacgatca gaactccgcc aaagtttttg tctatacact ctgcatttcg gccatggtga
     2941 acacggtggt gtgaaggtgt attcagcagc aggccgaaga agcggggcag gttgatcagc
     3001 tgcgtgtgca tcagaaactg gtaagacagc gacgctgcct gcatgagcag aatatgttct
     3061 ggcctgaagc cggcgaagcc cagtggcaac cagaaaatca taccgggaaa aggcagccag
     3121 gtttgcctca ggccgacggc gagattaaac tcattcgatg agtggtgcac aacatgggtc
     3181 atccacagga ctttcagccc gtggctcagc cggtgaaacc agtagaagca gaagtcatcg
     3241 gccaggaaca gcagcagcca gtaccaccat accacgggca ggcgggtaat tgttaagctg
     3301 agctgcaggt agaccagcag caggtattca agatagccgt aaatggcggc tacgatcagg
     3361 tggatcgtgt agtcggcgat attggtcatt gccccggcga agctgtagac ttttttgcgg
     3421 agtagcagcg acagcagcca ttcaatggta accagtgctg ttacgataaa taggataagt
     3481 ggcgctgtct gtgaccaggg catacgataa gcaccatgag ccagaatgcg ggggatgccc
     3541 tgtcaaatat ttcgccgtaa ggataaaaga gttttcacgc ccgctatgtc gattcacgct
     3601 gccgccgatg aagagcatag cacgcgatgc cttcacgttc ggcgtgcttc tgctgttgcc
     3661 aggctggctg gctgcagaac ctgtgattac cattcacgcc agtggattgc tggctgcgcg
     3721 cgtgctgcgt taccacacag cggggcagtt cagcgatgac gttacggtca tggttttttg
     3781 tccctgatcg catttacaag gccatttcag ccaccacgcc ttagccggca ttcgggttta
     3841 cgctctgtct gcaggtcaaa cagcaaaact gtaatggatg aagagatcga gcggctgacc
     3901 ggcattaccc gcgagcgggc tctgcgaatt gacgaagtga acctcgccga acggctgggg
     3961 cttgaaacta tccgttcgct cagtaccgaa ttctataccc gggtattcaa tgacgagcag
     4021 cagtggttca ggaacatctt caaacagagt accatcgaag aagcgataca gaaccagatg
     4081 gaattcttct tccagcgcct gggcggcgag ccgctctatt cacagcgcaa aggacaccct
     4141 gcgcttattg cgcggcatat gaaatttaat atgtcgctga aggcaacgga gcgctgggtt
     4201 tatcacatga agaacgcgct ggaagcaacc gaggcgatcg acgaagatgc gcgcgggcgt
     4261 atgctggatt ttttcacgca cacggcgtat ttcctgcata ttggctgcac aaccaggcag
     4321 cggcaaatgc atcagtcgtc gtaatcaaag aagaaatctt tattcgaaag aggctgcgaa
     4381 ccttcatggg tatggccggt tacgttttgt actatttccc gcaagatgcg tatttcctct
     4441 tcccaatagg catctgaagc aaaatcgggg aatgctgccg tgaatgaggg atcttcaaag
     4501 cggcgtgcaa tccagcccga atagtgcacg atgcgcattg cgcgcaagag ctccgcgtcg
     4561 gcaagccagc ggtcatcaaa atcccggaat tcacggtatg cctcgagaaa cacagcgcgt
     4621 tcatgctcgg gccgccccga aaacatcatc cagacatcct gcagtggggg cccgctgagt
     4681 gaatcgtcaa agtccagcag aaaccagcct tcgctgccct gcagcatatt gcccttgtgc
     4741 aggtcgccgt gtatgcgctg gtgcgggtgg ttcgctgaac gcacacgcca ttcatccgcc
     4801 aaatgcgcgg taagatcttc gtattctgcg gcgaatccct gtttaagaaa ttttctttcc
     4861 tgcagggcgt atagtggctc tacgaggtaa cgctccgcag aaaaagcagg gcggtgccgc
     4921 atattcgtgc cagcaccggt gttgtgcaag cgtgcaacca gccgccccag gatctgcaga
     4981 tcgatcgtgc tgagctcttc cggttcgcgg ccaccggtgc ggggccagac ggcaaaatag
     5041 attccctgcc gttcaaagag ggtgttaccg ccaattcgca gagggtagag cgcaggcact
     5101 tcgtcggccg cgagatcctc cagaaactgg tgttcttcga gaatctgttc tttgctccag
     5161 cgttgtggcc ggtaaaattt tgttaccaca tgccggccgt tttcgagccg caggtcgaag
     5221 acccgattct ccatgctgtt gagctgcgcg acgtgccccg aaggttcaag ccccagctca
     5281 cccagcgcgg cgactatttc atcgggtgtc agcctataga agaattccct gcgttcgtca
     5341 tcggccattg cgcagtgatg taacgagccg tctcagcgga ttgccattaa cagccgcgca
     5401 cggacaatct caggaaagat cttttcgatt tcgcgggccg gtattttttt gatggcacgg
     5461 gtaccagctt tcatctcgaa caggttatca gcttttgccg agagctccca cttttgctgt
     5521 gcatggctga ccatgacctg gccatcgatg agataaatac gggttgtacc cacctggctc
     5581 tgcagcagaa gcctggttcc tgtcaggcgt accaggttat cattggcaag cagtgctacg
     5641 gcaaactgcg gatcattgcg ctgcacgact gccgtgcctg tgagcaattg caccagtgtt
     5701 tcaccgctgg gttgttgtgt caggcgaacg acgcaatcgg ttgctgcttc gagcgtaaat
     5761 ttttccggca ggtagagtgt agcacccgcg ggcagaatct cgttgtgtgt tgaagctaat
     5821 ggttgtccgc caagaaacca gccctgcgga atgggagttt ttttggtact gcaggcgccg
     5881 aacaccagga tcaaggtcag cattgagtag gaaatgcgca ggcatgcgaa gctgaaatgt
     5941 cttgttacca taggtcctcc attcaggggc gcttaatggc attcggggat ttcatgttgc
     6001 gctgcacagt gtttttcggg agctgtcaat aagccccttt gccgctagcg catcagcttg
     6061 tcgacttcgc tggctttttc gaacgaggta tcctgcacga tcacaatctg tggaatcgcg
     6121 cgcatcctga tgttcttggc aatgctggtg cggagcgcag gcaccttctg cctgatatcc
     6181 ttcagcaggc gttcctgttc gtgagggttg ccccagacgc tgacgaaaag tttggcaaat
     6241 gagccatcgg cagacagctc acagcgtgtg aaggtcacgt aatcctgcac ctggcgcgtg
     6301 atatcttcaa tacccgccag ctcaaaaggg ttggtgatct tcgcttcttt atcctgcgcg
     6361 tcggccaaag tggttttggc tgactgcgat ggcgttgtct tctggaaaaa aagggcaacg
     6421 tggcgaaata tctggttctg caaccgcagt cgctttattt cattcatgac tgcctacagc
     6481 ccgcagcccg tctcagccct gcgtgcccgc aatgtcctca aaagtgcgct gggtctctac
     6541 cttttcaaaa gattcgatca cgtcgccaac cttgaggtca ttgtagttct cgaccaggat
     6601 accgcattca aagcc
//
LOCUS       DAOQJF010000178         6606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6496_length_6606_cov_4.842009, whole genome shotgun sequence.
ACCESSION   DAOQJF010000178 DAOQJF010000000
VERSION     DAOQJF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6496_length_6606_cov_4.842009"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLF85_10145"
     CDS             complement(<1..649)
                     /locus_tag="PLF85_10145"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HNE20071.1"
                     /translation="MTAITEINKIPPIPSGAVVTMGDFDGLHTGHRVLIDATVARSRQ
                     DGLTSVLVTYEPSPKKILKRLALDSRLTTFTEKQDVLRDTGLDLAVFFPVTADTLKIS
                     ARSFLRDFLLGRLHMKHIVMGNDHHFGHNRRGNAEYLLGACERYGFGVEIVEEQMTLE
                     RRTSSSRIRAALLEGDIDSVNAILGRPYSVSGPVVRGEARGRTLGFPTANVGLDPE"
     gene            complement(898..1242)
                     /locus_tag="PLF85_10150"
     CDS             complement(898..1242)
                     /locus_tag="PLF85_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20072.1"
                     /translation="MEELLKKIVNLGIGAAKGLEDNLQTAFKAAEQGINDLIAKGDTS
                     GEAGAGNVKKFVNDLLVSVRDYESTAREMSENLTTALREFQTSGQGRIEDLQKRVDEI
                     TSRIRSRMPGQS"
     gene            1408..2670
                     /locus_tag="PLF85_10155"
     CDS             1408..2670
                     /locus_tag="PLF85_10155"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNE20073.1"
                     /translation="MAQVAQKPLSLNRESDPFEHAQSENSYYGILNILYYLKAKYTRI
                     HFRYSGYVYIAHCESIGQQEVLLVAHGFQETQERRAVLQFEAYNRYYMSHVLVLRTNE
                     NGIYIQFPQQLLYLQRRRHIRLRFDDLFMRFTILYSPIGHTRLDEKNLESRFPYVMQE
                     IAREDVSLQTMYRMLVSEVQAISADFDIVFFASRPAEELTPAEKIVLETGNTFYVEDT
                     MRVISYTSPHGHRNLSNLSSLFEERAAAEGETAALRYVEEIRRSDARQFIVSYLLSPV
                     SLFGEIIGYVRLETNQFDKKYINQHQAMDMHRLMELFSYAMSKVRIRTSHFDPSSVET
                     RVVNISLSGLLLEFSDPAIFRYLKQHRRVKMLIPVMGDDLELYGEIVRFAERNGFYYL
                     GVLFFKSKPGDMIRLEKFIHENMQFQFL"
     gene            complement(2764..3105)
                     /locus_tag="PLF85_10160"
     CDS             complement(2764..3105)
                     /locus_tag="PLF85_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNE20074.1"
                     /translation="MKLITAMIQPNRLQDVQDALFKANVTKMTVSNALGCGQQKGYDE
                     SYRGAVHEVNLLKKVRIEIAVNEDYVEPTVNAIIAGARTGKIGDGKIFITELAEVIRI
                     RTGEKGKVAIG"
     gene            complement(3296..3637)
                     /locus_tag="PLF85_10165"
     CDS             complement(3296..3637)
                     /locus_tag="PLF85_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNE20075.1"
                     /translation="MKLITAMIQPYKLQDVKDALFKANVTKMTVSNALGCGQQKGYDE
                     TYRGVVHEVNLLKKVRIEIAVNDDFVEPTINAIISGARTGSIGDGKIFITELAEVIRI
                     RTGEKGKPAIG"
     gene            complement(3655..5127)
                     /locus_tag="PLF85_10170"
     CDS             complement(3655..5127)
                     /locus_tag="PLF85_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNE20076.1"
                     /translation="MKSLKLKLLFAGALALASAGLTHGAENTAAKPKELTLEQRVADI
                     EAYMNNVPRVKDDAAVKAADKAEKTNIAGAGPGHNAWIMTSALLVLFMTLPGLALFYG
                     GLVRTKNVLSVVAQCFGIAGLVTILWWAIGYSLAFSEGSPFIGGTAFAFFKGVDSSVG
                     QGGQWISNNVFSMFQMMFAIITPALIVGAIAERMKFSSILLFVAIWMFAVYFPLAHMV
                     WGPAGFMNGLGNFDKAGMKAIDFAGGTVVHMSSGWSALILAMILGKRNGYGAKSFAPH
                     NLVLTAVGTGMLWVGWYGFNAGSALAADGIAANAFTTTTLAAAVAAFVWPMLEWALKG
                     HPTVLGFCSGAVSGLVVITPAAGFVTASSAVTMGLIGGAVPFFAVMYMKKWLGYDDAL
                     DTFGVHGVGGTLGAILTAVFASTEANPALEGVIKNGLMGEHLKAIGFTIVLSVVATVI
                     IGYLLKFTIGLKASAETESLGLDISEHGEEGYNGFQTFVE"
     gene            complement(5244..5585)
                     /locus_tag="PLF85_10175"
     CDS             complement(5244..5585)
                     /locus_tag="PLF85_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNE20077.1"
                     /translation="MKLITAMIQPHKLQDVKDALFKANVTKMTVSNALGCGQQRGYDE
                     SYRGVVHEVNLLKKVRIEIAVNEDFVEPTINAIISGARTGNIGDGKIFITELVDVIRI
                     RTGEKGKAAVG"
     gene            complement(5626..>6606)
                     /locus_tag="PLF85_10180"
     CDS             complement(5626..>6606)
                     /locus_tag="PLF85_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia permease"
                     /protein_id="HNE20078.1"
                     /translation="FFFQLVFAGTAATIVSGAVAERIKFHSFIVFSIVLVAVLYPITG
                     HWVWGGGWLGAANFRDFAGSTVVHSVGGWAALAAVIVLGPRIGKYGPDGKVKPILGHN
                     MTSAALGTLILWLGWFGFNPGSFMGVGDGSGLSHVLLATNIAAATGAIAATATSWIML
                     KKPDLGMILNGTLAGLVAITAPCAFVTLPAAALIGAIGGALVVVGVLFFDKIKIDDPV
                     GATSVHLLNGIFGTLAAGFFYNHEIAKTVAGLTGDVVDPAKWSVGQQVMVQLKGIVAI
                     GGFTFVASLVLWYILKLAGGIRVSRDEEIMGLDVGEHGNEAYADFQEVNR"
BASE COUNT         1462 a         1804 c         1773 g         1567 t
ORIGIN      
        1 tttcggggtc aagccccacg ttagcagtgg gaaatcccag tgtgcgcccc cgggcttcac
       61 cccggacgac cggaccactg acagaataag gccggcccag aatcgcgttt acagaatcga
      121 tatcgccttc cagcaacgct gcccgtatac gcgaagacga agtgcgccgc tcgagagtca
      181 tttgttcttc gacgatttcg acgccgaagc cataacgttc acaggctccg agcaggtact
      241 ctgcgttgcc ccggcgattg tgcccaaaat ggtgatcgtt gcccatgacg atgtgcttca
      301 tatgcaaccg gccgagcaga aaatcacgca ggaatgaccg ggcagaaatt ttcagggtgt
      361 cggcagtcac cggaaaaaaa accgcaagat caagcccggt atcccggagc acatcctgtt
      421 tttcggtaaa agtcgtaagg cggctgtcca gagcgaggcg cttaagaatc ttcttgggac
      481 tcggttcgta agtcacgagg acagaagtca aaccatcctg tcgactgcgc gctacggttg
      541 cgtcaatgag cacgcggtgg ccggtgtgca ggccgtcgaa atctcccatt gtgacaactg
      601 cacctgatgg aatagggggg atcttgttga tttcagttat tgctgtcaca ggcacgccac
      661 cggcggttac ggggtctgct ccccataccc tttgcggtga gcaattcaaa agaggtttac
      721 agcgtgtcgt tacaaatacg tgcgttatgg atgccaagcg gccgtaaaag aaagagaaaa
      781 aaaatcgcca accacaagcg caagaagaag cgcagggcta accgtcataa aaagaaataa
      841 gttttagcgc attgccaccg gtaagggcca ggccccaccg gtggagaatc cgctcttcta
      901 ggattgcccg ggcatccgtg aacggatgcg actcgttatc tcatcgactc ttttttgcag
      961 gtcttcgatg cgtccctgtc cgctcgtctg gaactcgcgc agggcagtgg taagattctc
     1021 ggacatctcg cgcgcagttg actcgtagtc cctgaccgaa accagcagat cgttaacaaa
     1081 ctttttcaca ttgcctgcac ctgcctcgcc cgaagtatct cctttggcaa tcagatcgtt
     1141 gatcccctgt tctgctgcct tgaaggccgt ttggaggttg tcctccagtc ctttggcagc
     1201 gccgataccc agattgacga tttttttcag caattcttcc atgtttcccc ctgaaggtgt
     1261 ctttgtttgg cggcggagcg cccccgttct gcctgccgcg ggctttgtcc ccgggacaag
     1321 catttttgcc tgacaggcgg cagcctgctg gcgggaaaac gtcacggact tttcacttcg
     1381 ggctcccggg ttccgaaaat agatacagtg gcccaggtag cgcaaaaacc tctttcgctc
     1441 aaccgggagt ccgacccgtt tgagcacgcg cagtcggaga attcgtacta cggtattctc
     1501 aatatcctgt attacctgaa ggccaagtac acgcgtatac acttccggta ctcggggtac
     1561 gtctatattg cgcactgcga gagcatcggc cagcaggagg ttctgctcgt cgcgcacggc
     1621 ttccaggaga cccaggaacg ccgggcggtg ctgcagtttg aagcctataa ccgctactac
     1681 atgtcgcatg tccttgtact gaggactaac gaaaacggta tctatatcca gtttccgcag
     1741 caactgctct acctgcagcg gcggcgccat atcaggctca ggtttgatga cctgttcatg
     1801 cgctttacca tcctgtactc accgatcggg catacgcgtc tggatgaaaa aaatctcgaa
     1861 agccggttcc cctacgtgat gcaggagatc gcgcgcgaag atgtctcgct gcagacgatg
     1921 taccggatgc tggtcagcga ggtgcaggcg atcagcgcgg attttgacat agtctttttt
     1981 gccagccgcc ccgccgaaga gctgacgccg gcggaaaaga tagtgctgga aacgggcaat
     2041 actttctatg ttgaagatac gatgcgcgtg atttcttaca cgtcgccaca tgggcaccgg
     2101 aacctcagta acctgagcag cctgttcgaa gagcgcgctg ctgcagaagg agagaccgct
     2161 gcacttcgtt acgtcgaaga gatccggcgg tcagatgcaa ggcagttcat cgtctcgtac
     2221 ctgctgagcc cggtctcgct gtttggcgaa atcatcggtt acgtgcgtct cgagacgaat
     2281 cagttcgata agaaatacat taatcagcac caggcaatgg atatgcatcg cctcatggag
     2341 ttgttttctt atgcaatgag caaggtccgt attcggacgt cgcactttga tcccagttcg
     2401 gtagagacgc gtgtcgtgaa tatttctctt tcaggtctgc tgctcgagtt ttctgacccg
     2461 gcaattttcc gttacctgaa gcagcaccgc cgggtgaaga tgcttatacc tgttatgggt
     2521 gacgacctgg agctctatgg tgaaattgta cgatttgccg aacgcaatgg attttactac
     2581 ctgggcgttc tgttttttaa aagtaagccg ggcgacatga tccgcctgga gaagtttatt
     2641 cacgaaaaca tgcagttcca gtttttgtga caaccctgcg ggttgcccca aaaactggtt
     2701 aatgcatcct atccaatggt tgctgcctgg agcacgtcct gtgcgcagca acctacggac
     2761 gtcctatccg atagccactt ttcctttttc gcccgtacgt atacgaatta cttctgcaag
     2821 ttcggtgatg aaaattttgc cgtcgccgat tttgcctgtg cgcgcgccgg cgatgattgc
     2881 attgactgta ggttcgacgt aatcttcgtt cacggcgatt tcaatgcgga cctttttcag
     2941 cagattgact tcgtgcacgg cgccgcggta gctttcatcg taaccctttt gctggccaca
     3001 gccaagcgcg ttagaaacgg tcatcttggt gacattagcc ttaaaaagtg cgtcctggac
     3061 atcctgcagc cggtttggct gtatcatcgc agtaataagt ttcatggttc cctcctgctg
     3121 ctgccgggat ctgtgtgttt accggctttt agccgggccc tgctgcacgg gaatcattat
     3181 aagaaagcgg gatagggggg gaagtggaag tatttcccct ttcccccaaa ggacatccta
     3241 tcctataggt ctgcggcatg gatgacatcg tgtcagccgc agaccaaagg acatcctatc
     3301 ctattgccgg tttgcctttt tcgcccgtac ggatacggat gacttcagcc agttcggtga
     3361 tgaatatttt gccgtcgccg atgctgcccg tgcgtgctcc gctgatgatc gcgtttatcg
     3421 tgggctcaac gaaatcatcg tttacggcga tttcgatgcg gaccttcttg agcaggttca
     3481 cctcgtggac cacaccgcgg taggtttcat cgtagccttt ctgctggccg cagccgagcg
     3541 cgttcgagac ggtcatcttg gtgacgtttg ccttgaaaag tgcatctttg acgtcctgca
     3601 gtttgtaagg ttgtatcata gctgtaatga gtttcatagc gtctttcctc ctggttattc
     3661 aacaaatgtc tgaaagccgt tatagccttc ttcaccgtgt tcgctgatgt cgagaccgag
     3721 cgattcagtt tcagcgctcg ccttaaggcc gatggtgaat ttcagcaggt agccaatgat
     3781 gactgtagcg acaactgaca ggacaatcgt aaatccaatt gccttcaggt gttcacccat
     3841 gaggccgttc ttgataacgc cttccagcgc agggttggct tcggtgctgg cgaaaactgc
     3901 ggtgagtatc gcgcccagtg taccgcccac gccgtgtacg ccaaaggtgt ccagcgcgtc
     3961 atcataaccc agccacttct tcatgtacat gactgcgaaa aacggtacgg caccgccaat
     4021 gaggcccatc gttactgctg atgatgcggt gacgaaacct gcagcaggtg tgatcacaac
     4081 caggcccgag actgcgcccg agcagaagcc aagtactgtc gggtgtccct taagagccca
     4141 ttcgagcatc ggccagacaa aagcggctac agcggcagcg agtgttgttg tggtaaaagc
     4201 attggctgca attccgtctg cggcgagagc cgagcctgcg ttaaaaccat accagccaac
     4261 ccagagcatt cccgtgccga ctgcggtgag aacgaggttg tgcggtgcga aggatttggc
     4321 gccgtaaccg ttgcgctttc cgaggatcat cgccagaata agtgctgacc agcccgatga
     4381 catgtgtacc accgtgccgc cggcaaagtc gattgctttc attcccgctt tgtcaaagtt
     4441 accgaggccg ttcataaagc ctgcggggcc ccataccatg tgcgccaggg ggaaatatac
     4501 ggcgaacatc cagatggcga cgaagagcag tatgctcgaa aatttcatcc gttcggcaat
     4561 tgctcccacg atgagtgccg gggtaataat ggcgaacatc atctggaaca tagaaaagac
     4621 gttgttcgaa atccactggc caccttgtcc gactgagctg tcgacacctt tgaaaaatgc
     4681 aaaggctgta ccgccgataa aggggctgcc ctccgagaag gcgagtgaat agccaatggc
     4741 ccaccacaat attgtgacga gaccagcgat gccaaagcac tgcgcgacaa cgctcagtac
     4801 gttctttgtg cgcacgaggc cgccgtagaa gagggcgagt cctggcagcg tcatgaagag
     4861 caccagcagc gcagaggtca tgatccaggc gttatggccc gggcctgcac ctgcgatgtt
     4921 tgtcttttcc gctttatctg cggcttttac cgctgcatcg tctttgacgc gcggtacatt
     4981 attcatgtaa gcttcaatat cagcgacccg ctgttcgagg gtcagttctt taggcttggc
     5041 agcggtgttt tccgcgccgt gcgtgaggcc agccgaggca agtgccaggg cgcccgcgaa
     5101 gagtaatttc agtttgaggg atttcatgcg ttctcctgag ttgttttgtt gttgtatctc
     5161 ttttcctttt tctgactgtg cgcgcacctt atcggggcgt cgacaaaaaa accgcggctt
     5221 tgtgggccgc ggcgaggcac cggttatcct accgctgctt ttcctttttc acccgtacgg
     5281 atacggatta cgtctaccag ctcagtgatg aagatttttc catcaccgat gtttccggtc
     5341 cgtgcaccgc tgatgatcgc gttgatcgtc ggttcgacga agtcttcgtt gaccgcaatt
     5401 tcaatgcgta ctttcttcag caggtttact tcatgaacca caccgcggta cgattcgtcg
     5461 taaccacgct gttgtccgca accgagagcg ttcgagacgg tcatctttgt gacgttcgcc
     5521 ttgaacagtg catccttcac atcctggagt ttgtgaggtt gaatcatggc tgttatcagt
     5581 ttcatatttt tctccttgtg gagctaaccg gccaatcctc gcggatcagc ggttaacctc
     5641 ctggaagtct gcataggctt cgttgccatg ctcgcccacg tcgagaccca tgatctcttc
     5701 atcccgtgat acgcggatac cacctgcaag tttcaggatg taccagagca ccagtgaagc
     5761 gacgaacgtg aagccgccga ttgctacaat acccttgagc tgtaccatca cctgttggcc
     5821 aacagaccac ttagcaggat ctaccacatc gccggtcagg cctgcaacag tcttcgcgat
     5881 ttcgtggtta taaaagaaac cagcggcaag agtaccgaaa ataccgttca gcaggtgaac
     5941 cgaggttgct cccaccgggt cgtcgatctt gatcttgtcg aaaaacagta cgcctaccac
     6001 aacgagcgct ccgccgattg cgccaatcag cgctgcggcc ggcagggtta cgaatgcgca
     6061 gggggcagta attgccacca ggcctgcaag tgtaccgttg agaatcatac cgaggtcagg
     6121 tttcttcagc ataatccacg aagttgccgt tgcggcgatc gcaccggtcg cggctgcgat
     6181 gttggtcgcg agcagcacgt gcgagagtcc cgagccgtcg ccaacgccca tgaagctacc
     6241 ggggttaaag ccgaaccaac caagccacag gatcagtgta cccagagctg cagaggtcat
     6301 gttgtggccg agaatcggtt tcacttttcc atcaggacca tattttccga tacgaggacc
     6361 aagtacaatc actgcagcga gagctgccca gccgcccacg ctgtgaacca cagtcgagcc
     6421 ggcaaagtca cggaagttcg ctgcgccgag ccagccgccg ccccagaccc agtggcctgt
     6481 gatggggtag agtaccgcca ccagcacgat gctgaacaca atgaatgagt ggaacttgat
     6541 acgttctgcg actgctcccg aaacgatcgt ggcagcagtg ccggcaaaca ccagctggaa
     6601 gaagaa
//
LOCUS       DAOQJF010000179         6599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6507_length_6599_cov_5.534535, whole genome shotgun sequence.
ACCESSION   DAOQJF010000179 DAOQJF010000000
VERSION     DAOQJF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6507_length_6599_cov_5.534535"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..918)
                     /locus_tag="PLF85_10185"
     CDS             complement(151..918)
                     /locus_tag="PLF85_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE20079.1"
                     /translation="MDVQGYRKLGCEECLKPELLGFDFTMAFQPVIDAETRSVYSHEA
                     LVRGLNGEGAASVLGQVNESNRYRFDQACRVKAIEAASRLNLKTFLNINFMPGAIYNP
                     QTCIRTTLEAAEKYGFPKERIVFEVTEGERVTDSNHLKAIFAEYRKHGFQTAIDDFGA
                     GFAGLNLLAEFQPEIVKLDIALIRQLDTDRVRRVIVEAIHQACTELGIRVVAEGVESI
                     AEYQVLRQMGVRYFQGYLFARPALDPVSASVNIPATP"
     gene            1068..2117
                     /locus_tag="PLF85_10190"
     CDS             1068..2117
                     /locus_tag="PLF85_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE20080.1"
                     /translation="MTHSTDSSRQVAVLLNANARLVTDKVKRSLERIVPRANLFLSHT
                     KEEALDYVKHIIDNRYATIFSGGGDGSFVHLLNLARDYLKGKKRPGEAEPELPQFGIL
                     KLGTGNGISSFVGSRGGTRLLERSLHAHHLDTLEMSLIERNDRIFHFAGSGFDARLLK
                     DYYDFMDSLKTPAARKNFSGLVGYFWSGLGKTVPESIIKPDRGEVRVEIETGDQGFYV
                     KHTHGRDQAVATESTLLYSGPSTAVAVATEPFYGYNVRAFPFATMKPGFMNLRVLLAQ
                     PVSLVANMRDYWNGTYRGRGVKDFLVRKVKITYEKPTPIQVGGDYDSLADSITYSIFP
                     QKCKLVDFRHLAHLA"
     gene            2166..2888
                     /locus_tag="PLF85_10195"
     CDS             2166..2888
                     /locus_tag="PLF85_10195"
                     /inference="COORDINATES: protein motif:HMM:NF021449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HNE20081.1"
                     /translation="MSHRRAGKTPEPSEADLYPIAARYFAAQGFQVKGEVCGCDLVAV
                     KDNTIIVAELKKTFNIKLLYQSVRRLAITDQVYAVIYKPKGRQKMSFWQMIKSLARRL
                     NIGLLVIDGDKVMVIAEPGDFTGRVLARHKNKVLKEFHGRRVSENTGGVTGVKLQTAY
                     LESAIHISVLLKKHKAMQPAQLVKVGVSANARQILYNNHYGWFEKSARGLYKLKTGKA
                     RVIARENPTIWEYYEKLVDDTE"
     gene            2972..3853
                     /locus_tag="PLF85_10200"
     CDS             2972..3853
                     /locus_tag="PLF85_10200"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE20082.1"
                     /translation="MNFSIATWNCFGMGQSVIDVITYLRAPFGKRFSSPEVIDECSSA
                     NVLCVQELLSRASQVFFDNLVRRYFPFALRDHNRFGLLSLRGSGLGIAAREPATEGAK
                     TRKLQSRFLPFRSRGTSWDKYARKGALYAQFEYADRLVDVMTVHLQSGMDAASSAVRA
                     AQLAELAEWIKWLGSSGRPFIVCGDFNIQGMAADRESGEYKTLTAALGGFTDLGAESD
                     LPTYHPHPEGNPLAHAFEAEGEPMRIDYIFLRPAETGQAFTPSGFRRILDRPLSGLTG
                     LGAWASDHYGLVATFEV"
     gene            3850..4419
                     /locus_tag="PLF85_10205"
     CDS             3850..4419
                     /locus_tag="PLF85_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNE20083.1"
                     /translation="MNNYTLWFLAILPPADVSSRVRDIQQEIADNFGPRRAMKIPVHI
                     TVEPPFRRDATDADWMSERLSAFFATESAFVLELRDFGAFRDDVIFIDVAANLQLLEM
                     HQRLSDCLRAQPAIIETAPFHTGYKPHMTVANRDVEPDVHRHIWNEFKTRKFYARFPV
                     TEINLLRHDGKEWHIYRQFALRREQDANL"
     gene            4406..5062
                     /locus_tag="PLF85_10210"
     CDS             4406..5062
                     /locus_tag="PLF85_10210"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNE20084.1"
                     /translation="MQTFRSDISGREYPIHDRVPGSVIRDTVMAVIQREHPHFGDDSQ
                     ISASELNYYRKKYLSEYLTEQSGDLTEAEKQVVSAVAAKSTLTDKVDAESEAGLTFGQ
                     RVADKVADFGGSWTFILSFMGFLALWIAANAWWLSHRSFDPYPFILLNLILSCLAALQ
                     APVIMMSQNRQDEKDRERAKKDYMVNLKSELEIRMLHEKIDHLISEQQGIREQLNKKQ
                     "
     gene            5113..5472
                     /locus_tag="PLF85_10215"
     CDS             5113..5472
                     /locus_tag="PLF85_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HNE20085.1"
                     /translation="MALNVLGTELLPCSMEPLTGFFRDGCCNTSSEDVGMHTVCVEVT
                     EEFLEFSKAAGNDLSTPVPQYMFDGLQPGDRWCLCMPRWIEAYNANMAPRIVLEATHV
                     SVIEHIDLDLLKRFAVG"
     gene            5478..6281
                     /locus_tag="PLF85_10220"
     CDS             5478..6281
                     /locus_tag="PLF85_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE20086.1"
                     /translation="MKRLLTLLLTILPLTLFAEDGQLTWLKLNTPHRLIGDKVRVRSA
                     PDSKAKVLAELSIGIEIVPLEQTSSTMTVDGVQAPWYKVRYSRDGGHPEGYVWGNLIA
                     RAFAESKTGEVFLFGTGRRQKDISVGTEYTSQVRVALDGVELARLEVKEGTSFEARYE
                     VTLSNGRGLEGVSNIFAVTFVQEYCAGKGNTIFFFWNGKKLLHAHSSVDGADAPYYAM
                     EKQIFPADKGGRKGFVILEQEHGDHDDPKSVKKEKILLKWNGKKLEKSS"
     gene            6278..>6599
                     /locus_tag="PLF85_10225"
     CDS             6278..>6599
                     /locus_tag="PLF85_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20087.1"
                     /translation="MKQCMTIIALVAISAGSLAAVKKQRLHAVEVTYESIRGHTESYG
                     SSEKHVVVVLVNGGEKDTEVSGFVTAKNTAKGYTVASCPFKTGVPAGSEVKKKIKCQV
                     ADADS"
BASE COUNT         1586 a         1832 c         1789 g         1392 t
ORIGIN      
        1 gagccggccg ctgagttctt cgccctgcct gacatcgctg ggaatcgtga atttacccct
       61 taaatgatcc ataggccggc gtttgattat cggagtaata ccctgctcaa atcaaatacg
      121 ctgggagcag atatcgaata atttttgcta tcagggcgtt gcgggaatat tgacgctcgc
      181 agacacagga tctaatgccg gtctggcaaa caagtaaccc tgaaaatagc gcactcccat
      241 ttgccgcaat acctggtact ctgcgatgct ctctacaccc tcagcgacaa cgcgaatgcc
      301 gagctcggta caggcctggt gaatagcctc aacaatgacc cgtcgtacgc ggtcggtatc
      361 gagctggcga atcagcgcta tgtcgagttt cacgatttcg ggctgaaact cggcgagcag
      421 gttcaggccc gcaaagcctg cgccaaagtc atcaatcgct gtctggaagc catgtttacg
      481 gtactcggca aaaattgcct tcaggtgatt actgtctgtc acgcgctcgc cttcggtaac
      541 ctcaaaaaca atacgctcct tgggaaagcc atacttctct gctgcctcga gggttgttct
      601 tatgcaggtt tgcggattgt agatcgcgcc cggcataaag ttgatgttga gaaatgtttt
      661 aagattcaga cgggatgctg cttcaatcgc tttgacccgg caggcctggt caaagcggta
      721 gcggttcgat tcgttgacct gacccaacac gctcgctgcc ccttcgccgt tcaacccgcg
      781 caccagcgct tcgtgcgaat agacactgcg ggtttcggca tcaatgaccg gctggaatgc
      841 catggtaaaa tcaaagccaa gtaattcagg cttcaggcat tcttcgcagc ccagctttcg
      901 gtatccctgc acatccataa agagggaaaa aagttcctat gacaaccctt gcgtcaagaa
      961 aaattgaact ccttcgccaa ctgcaacctt gcgagagata gcatagttaa cgacgttaac
     1021 catataaaaa attgacaccg ccgtcagtaa gtcaaattgg ccgggtagtg actcactcta
     1081 ccgatagcag ccgacaggtc gcggtgctgc tcaatgccaa cgcccgcctc gtgaccgaca
     1141 aggtcaaacg ctcgctcgag cgcattgtac ccagggcgaa cctgtttctc agccatacta
     1201 aagaagaagc cctcgactat gtgaagcaca tcatcgacaa tcgctacgcg acaatcttct
     1261 cgggcggggg cgacggcagc ttcgtgcacc tgctgaacct cgcgcgcgat tacctgaaag
     1321 gcaagaaacg cccgggtgaa gcagaacctg aactgccgca gttcggcatc ctgaagctcg
     1381 gcaccggtaa cggtatctcg agttttgtcg gttcgcgcgg cggcaccagg ctgctcgagc
     1441 gctcgctgca cgcgcaccat ctcgatacac tcgaaatgtc cctcattgag cgcaacgacc
     1501 gcatcttcca cttcgccggc agcggcttcg acgcgcgcct gctgaaagac tactacgact
     1561 tcatggacag cctcaagacc cccgctgcgc gcaagaactt ctcgggcctg gtcggttact
     1621 tctggtcggg cttgggtaaa accgtacccg aatcgattat caaacccgac cgcggcgagg
     1681 tgcgggtaga aattgaaacc ggcgaccagg gcttctacgt gaagcacacg cacggccgcg
     1741 accaggcagt cgccaccgaa tcgacgctgc tctactcggg cccatcgact gcagtcgccg
     1801 tcgcaaccga acccttctac ggctacaacg tgcgcgcatt ccccttcgcg accatgaagc
     1861 cgggcttcat gaacctgcgc gtactactgg ctcagcccgt aagcctcgtg gcgaacatgc
     1921 gcgactactg gaacggcacc taccgcggcc ggggcgtgaa ggatttcctg gtgcggaaag
     1981 taaagatcac gtacgagaag cccacaccga tacaggtggg cggtgactac gattcgctgg
     2041 cagattcgat tacctactcg atttttccgc agaagtgtaa gctcgtcgat tttcggcatc
     2101 tcgcgcacct cgcctgagta ccgttttctc actgacgttt agtaggtact aaagagtctg
     2161 gtcttatgtc acatcgccgg gcaggcaaaa ccccggaacc cagtgaagcc gacctctacc
     2221 cgatcgcggc gcgctacttt gccgcgcagg ggttccaggt aaaaggcgaa gtctgcggct
     2281 gtgatctcgt ggcggtgaaa gataacacga tcatcgtcgc cgaattaaag aaaacattca
     2341 atatcaagct cctctatcag tcggtgcgcc ggctcgcgat caccgaccag gtctacgcgg
     2401 tgatttataa accgaaaggc cggcaaaaga tgtcgttctg gcagatgatc aaaagcctcg
     2461 cgcggcggct caacatcggc ctgctggtga tcgacggcga caaggtgatg gtcatcgccg
     2521 aaccgggcga cttcacgggc cgcgtgctcg cgcgacacaa gaacaaggtc ctgaaagaat
     2581 ttcacgggcg gcgtgtttca gaaaacaccg ggggagttac aggcgttaag ctgcagaccg
     2641 cgtaccttga gtcggcgatc cacatcagcg tgctgctcaa gaagcacaag gcaatgcagc
     2701 ccgcgcagct cgtcaaagtg ggagtttctg cgaacgcacg gcaaattctc tataacaacc
     2761 actatggctg gtttgaaaaa agcgcgcgtg ggttgtacaa gctcaaaaca ggcaaagcgc
     2821 gcgtcattgc cagggaaaat cccaccatat gggaatacta cgagaaactg gttgacgaca
     2881 ccgaataggg gcggacgatc tgctggttgg ccaaatcttt gcttatcagc aaacagatgg
     2941 taaaccacac gcaacaggtc gagaattaag tatgaacttt tcaatcgcta catggaactg
     3001 ctttggcatg ggacagtcgg ttatcgacgt tatcacgtac ctgcgcgcac cgtttggcaa
     3061 gcgcttcagc agcccagagg ttatcgacga atgttcgtcg gcaaatgtgc tctgtgtgca
     3121 agaactgctc tcgcgcgcct cgcaggtctt tttcgacaat ctcgtgcgcc gctactttcc
     3181 gtttgcgctg cgcgaccata accgcttcgg cctgctttcg ctaagaggca gcgggctggg
     3241 cattgccgcg cgtgagccag ccacagaagg cgcaaagacc cgcaaactgc agtcgcgctt
     3301 tctacccttc agaagccgcg gcaccagctg ggacaaatac gctcgcaaag gcgcactcta
     3361 tgcgcagttt gaatatgccg accgcctggt cgacgtgatg accgttcacc tgcagtcagg
     3421 tatggatgca gcttcaagcg cggtgcgtgc ggcgcagctc gctgaactcg cagaatggat
     3481 taagtggctg ggctcttcgg gcaggccatt tatcgtgtgc ggcgatttta atatccaggg
     3541 catggcggca gaccgcgaat cgggtgaata caagaccctc actgcagcac tcggtggctt
     3601 cactgatctc ggtgcagaat ctgacctgcc aacataccat ccgcaccccg agggaaatcc
     3661 cctcgcgcat gcttttgaag cagaaggcga gccgatgcgt atcgactaca ttttcctgcg
     3721 gccggcagag accggccagg cattcactcc atcgggcttc cgccgcattc ttgaccggcc
     3781 actatcggga ctaaccgggt tgggagcctg ggcgtctgat cactatggac tggttgcaac
     3841 ttttgaagtg tgaataacta cacactctgg tttctggcaa ttctgccgcc ggccgacgtt
     3901 tcatcgcggg tacgcgacat ccagcaggaa atcgcggata acttcgggcc gcggcgtgcg
     3961 atgaagatcc ccgtgcacat cacggtcgaa ccgccgtttc gccgcgacgc caccgacgct
     4021 gactggatgt ctgaaaggct ttcggcattc tttgcgaccg agagtgcatt cgtgcttgag
     4081 ctacgcgact tcggtgcatt ccgcgatgac gtcatcttca tcgacgtcgc agccaacctg
     4141 cagctgctcg aaatgcacca gcggctgagc gactgcctgc gtgcgcagcc ggcgattatt
     4201 gaaactgccc cgtttcatac cggctacaag cctcacatga ccgtcgcaaa ccgcgatgtc
     4261 gagcccgacg tgcacaggca catctggaac gaattcaaaa cgcgaaaatt ctatgcacga
     4321 tttccggtca cagaaatcaa cctactgcgc cacgatggaa aggaatggca tatctacagg
     4381 cagtttgcgt taaggcgtga acaggatgca aacctttaga agcgatatat cgggccgtga
     4441 atacccgatc catgaccggg tgccgggaag cgtcattcgt gacacggtca tggctgtcat
     4501 ccaaagggaa catccgcact ttggcgacga cagccagatt tctgccagcg aactcaacta
     4561 ctaccgcaag aaatacctat cggagtacct gacagagcag agcggcgatc tgaccgaggc
     4621 agaaaaacag gttgtctctg ctgttgccgc gaagagtacg ctgaccgaca aggttgacgc
     4681 tgaaagcgaa gcagggttga cgttcggcca gcgtgtcgcc gacaaggtcg ccgattttgg
     4741 cggcagctgg acttttatat tgtcattcat gggtttcctt gctctttgga tagctgccaa
     4801 tgcctggtgg ctttctcatc gctcgtttga tccttatcca ttcatactac tgaacctgat
     4861 tctttcctgc ctcgccgcgc tgcaggcacc ggtcatcatg atgagccaga accgccagga
     4921 cgaaaaagac cgcgaacggg caaagaaaga ctacatggtt aacctgaagt ccgaactcga
     4981 aatacgcatg ctgcacgaaa aaatcgacca cctgatcagc gaacagcagg gtatacgcga
     5041 gcagctgaat aagaagcaat aagtacctgc caattgcgtt gacgccgcac aaagcgtgca
     5101 aatgcagaaa gtatggcact caacgtcctc ggtaccgaac ttttaccctg cagcatggag
     5161 ccgctgacgg gttttttccg cgacgggtgc tgcaatacca gctcggaaga tgtcggtatg
     5221 cacaccgtct gcgtcgaagt cacagaagaa tttcttgaat tcagcaaagc tgccggcaac
     5281 gacctttcga ccccggtacc acaatatatg ttcgatggcc tgcagcccgg cgatcgctgg
     5341 tgcctgtgta tgccccgctg gatcgaagcg tataatgcga acatggcgcc gcgcattgtg
     5401 ctcgaagcca cgcatgtctc ggtaatcgaa catattgatc ttgacctgct caaacgtttt
     5461 gcagtcgggt gagggacatg aaacgattat tgactcttct gcttactata cttccgctta
     5521 cactcttcgc cgaagacggg cagcttacct ggctcaaact caatacaccg caccggctga
     5581 tcggtgacaa ggtacgcgtt cgcagcgcgc cggatagcaa agcaaaagtt ctggcggagc
     5641 tctctattgg catcgaaatt gtgccgctgg agcaaaccag cagcaccatg acggtcgatg
     5701 gcgttcaggc gccgtggtac aaagtacgct attcccgtga cggggggcat ccagaaggtt
     5761 atgtctgggg caacctgatt gcacgggcat ttgctgaaag caagacgggg gaggtattcc
     5821 tgttcggcac cggccgccga cagaaagata tctccgtcgg taccgaatac acttcgcagg
     5881 tacgcgtggc gcttgatggt gtcgaacttg cccgcctcga ggttaaagag ggtacatcgt
     5941 tcgaggcccg gtatgaagtg accttatcca atggccgcgg tcttgaaggt gtcagcaaca
     6001 tcttcgcggt gacgtttgtc caggaatact gtgccggcaa aggcaatacg attttcttct
     6061 tctggaatgg aaagaagctg ctgcatgcgc acagctctgt tgacggagct gacgcaccct
     6121 actatgcaat ggaaaaacaa atttttcccg ctgacaaggg tggccgtaag gggtttgtca
     6181 ttctcgagca ggagcatggc gaccatgacg acccgaaatc cgtcaaaaaa gaaaagattc
     6241 ttttgaaatg gaatggcaag aaactggaga aaagctcatg aaacagtgta tgacgataat
     6301 tgcgctggtg gcaatttcag cggggtctct ggctgctgta aaaaaacagc ggctgcacgc
     6361 ggttgaagtc acgtacgaga gcatacgtgg ccatacagag tcgtacggct catcagaaaa
     6421 gcacgtggtc gttgtgctgg tcaatggcgg tgaaaaagat accgaagtat cgggcttcgt
     6481 tacagcaaag aacaccgcaa agggctacac tgtcgcgagc tgcccgttca agactggtgt
     6541 gcccgcgggc tctgaggtga aaaaaaagat caaatgccag gttgcagatg ctgattcac
//
LOCUS       DAOQJF010000180         6582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6531_length_6582_cov_4.461774, whole genome shotgun sequence.
ACCESSION   DAOQJF010000180 DAOQJF010000000
VERSION     DAOQJF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6531_length_6582_cov_4.461774"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLF85_10230"
     CDS             <1..476
                     /locus_tag="PLF85_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20088.1"
                     /translation="RGGILNTQAAISLSAASGLLDILGYARENNEAQHFFFGGNGGID
                     TINSVALLSRAAEIPVPATISRPTVPAECRPGMEGEELRLYAAVMVRFNPGTEVPFAL
                     GAFRRSDGTLFLARILDSAGQTPTDTDQFVRDKALFRLRVENNDTVFAIEVKTEG"
     gene            complement(477..1109)
                     /locus_tag="PLF85_10235"
     CDS             complement(477..1109)
                     /locus_tag="PLF85_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20089.1"
                     /translation="MEISGQRLFFPPNFRFFNRLPARFEILVIARGANKQGSTVFEVE
                     IAGQRLALPSRVALVPGKRYELEKVSELEFRIVREKAVTEKEAEQLPRLREPNEVATQ
                     STDAGIPNITPFSLGELAILRLLEEEGRSVREQNQKYLFDFSGEFELRGLFHSHRDGS
                     YTLFVSGSLAAGAFPGALSDELKSLGISGIKVLDSAVFDRLAAGAIDLSL"
     gene            complement(1128..2267)
                     /locus_tag="PLF85_10240"
     CDS             complement(1128..2267)
                     /locus_tag="PLF85_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNE20090.1"
                     /translation="MKQKTSTQTISRILIAVTALLFAGHNASAQVPDDLIPRLLADCP
                     ESVAKSYATSVPRFARVLPEKKADPAATLAAVVPRACYLELQDWQLQTALQLGHYGLN
                     QGLKASTISDLTEVLSWRAIAKESYVRLGHVYEHMQKAGASDEEIAEVFLQAQNSGLL
                     ADQTEAYSATYAKARSAGKGHNDAIAIAAEELPRLRKVRGERNIQQFMAEISGGAAAR
                     SGGAVQPLTGDALWDQLESTIKAEQVQPAAANAQAAWNLPRLETFFDEWKGTPYRWGG
                     VTRKGIDCSGFVLKAIESQFPQSKYPRSARDLAAQGSEVPRSGLKTGDLVFFAASDTP
                     GRITHVGIVIKGSQFAHASSKRGVTLSNLDDKYYTQRFVTARRLF"
     gene            complement(2264..2761)
                     /locus_tag="PLF85_10245"
     CDS             complement(2264..2761)
                     /locus_tag="PLF85_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE20091.1"
                     /translation="MTGGAFLQLRFTSISPGKLADLAYAPFRNQFADKDISVEFNIPY
                     VLPTLHCDVAKIAWVMTIFFSNAVRYTPQWGKLAVKGSDAHGNLRLAVENSGYGIPPE
                     RLGQMFEATENPEAPEFGRGLALLLARDIVEAHGGKIGVYTEPGVLTGFFIELPIIRP
                     EAESP"
     gene            complement(2850..3842)
                     /locus_tag="PLF85_10250"
     CDS             complement(2850..3842)
                     /locus_tag="PLF85_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20092.1"
                     /translation="MRKTTAFLILLAAAPLSYAENVAEKAKYKRAEALEQIVYLDVPL
                     RNFGSEKPELVKLYDDVKQKYAVALSFFFEDNFVESYKLFLDVQNTLEKLYEQVSMFY
                     IDRTNIVLQQSVKEATEIEIRFNRKAPFATQKIGKEREAGVRISADKKQTQLEKRLYD
                     PKDIHYLYDKKAMIDNIDYGYMQLGQAKVARQKAMDLEKWLEKGKAMPPTMKKERINT
                     YKAVIDLCRQAKVNGIMVMQLNRIFDNYELQTKFKDNYFMKEKRLDPIFDFAIPEDYR
                     KDANDSRNRIHDHEDRIKLKGEDPAKIAQEDRKPLQGSKTEGNKPAASDSNKPK"
     gene            3983..>6582
                     /locus_tag="PLF85_10255"
     CDS             3983..>6582
                     /locus_tag="PLF85_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20093.1"
                     /translation="MPSGHDRKVQERRDYFCPRLNLQARYTGLKKSLALLVLLLLGVV
                     SPLTARSWDDLNEGETEIYGREREEKLPLRLFFVQREKWQDHDALHILWLYGYKDYPR
                     YSSRRLLPFYYRLESRVDNRRLTFTPISLTETDGESESSIWFWLFFRGEDRAAKKSYS
                     ALFPFYYRESLADASSVYWLTPLFWYSRTKAPLGDGYSFGAPVIPLVMLNREATYSDL
                     TVFYIFRQRSGQQTLSYLLPLWYYHSQPQGYLVSVLPPIAWYERSGNSSWLLVFPLWY
                     SSHHPGGSTVASPLYLRSESADSRLNAFIPLWFSYSRKNYHLHINAAGISLAEEQFSA
                     LPFSATLAGNRLYADWDFGWFYSLFHVSSRSSIALADTATEENKIPIANTGITERRRR
                     NRADSESFFGLYALFGALAYERADHYRHFRLLPLSWLTWDSRSDTGVQTVIPFYVHYR
                     DAESRYLVIAPLLVPFYASQETTQSRLPEDNQELPAISAPATAAAPATGKCRSDKQAW
                     LMIMFIREYDCSLEEREQTLLWPVLNRYTSPQRGGFRVFPVFWSKWWQTTVGEERWNF
                     SPLHYSRSLNDNYRLLTWLFYYLRNETATSYGTWGIWHIGRTPVTSTTYFFPVWLRTA
                     QEPGDRATGRAGYHDSLLTVAGLWWRSSSTMGSDPARTVHVSPLYLYFASGSGTDFYS
                     WLYYRTDHPSISRHGIPLLFNHTSRGDTYRNFYLFPFYRSVELLSNDETEYTSWLLPL
                     WYSRSDGKSAERFIAGYWYERRPGFALDNFLLMAGRSDDDASGRHSRHALLYTFRYDY
                     TPTESRLNLLYGLLFSREQTEWEFSWHFALITGYRITPDGVYRRHHLLPAYWYSVSDD
                     ERMLLLPALLS"
BASE COUNT         1387 a         1891 c         1741 g         1563 t
ORIGIN      
        1 ggcgcggtgg aattctcaat actcaggcag cgatttcact ctctgctgcc agcggcctgc
       61 tggatatact cggatacgcc cgcgagaata atgaggcgca acatttcttc tttggtggaa
      121 acggcggaat cgataccatc aactctgtcg ccctgctttc acgtgcagct gaaataccgg
      181 taccggcgac aatatcccgg cccacagtac ccgctgaatg ccgccctggt atggagggcg
      241 aggagctcag gctttacgcc gcagtgatgg tccggtttaa tccgggcacc gaggtaccgt
      301 tcgcgctggg ggcatttcgt cgcagcgatg gcacactttt tctcgctcgc atactggatt
      361 ccgcgggcca aacgcctacc gatacagacc aattcgttcg cgacaaagca ctgtttcggt
      421 tgcgcgtgga gaacaacgat actgtttttg cgattgaagt aaagacagaa ggctagttag
      481 aggctcaggt cgatcgctcc ggccgcaagc cggtcaaaga cagcgctgtc gagcaccttg
      541 atgccgctga taccaagcga cttgagttca tccgacagtg cgccaggaaa agctcctgcg
      601 gcaaggctgc cggaaacaaa aagcgtataa ctgccgtccc ggtgtgagtg gaatagaccg
      661 cgcagctcga actcacccga aaagtcaaag aggtatttct ggttctgttc gcgcaccgac
      721 cgcccctctt cttcgagcag gcgcaggata gcgagctcgc ctaacgaaaa aggcgtaata
      781 ttcggaatcc ccgcatcagt actttgtgta gcaacttcgt taggttcgcg cagcctgggc
      841 aactgctctg cttctttttc cgtgacagct ttttcccgca caatgcggaa ttcgagttca
      901 gagacttttt ccagttcata acgttttccg ggtacaaggg caacacgcga aggcagtgcg
      961 agtctttgcc cggcaatttc gacttcaaag acagtgctgc cctgcttgtt ggcgcccctg
     1021 gcaatgacga gaatttcaaa acgcgccggc aggcggttga agaacctgaa gttcggggga
     1081 aagaagagtc tctgccccga aatctccatc acagtcctgg gccgctatca gaatagacgg
     1141 cgcgcagtca cgaaacgctg ggtgtagtac ttgtcgtcaa ggttggaaag agtgacgccg
     1201 cgctttgaag aggcgtgcgc aaactggctt cccttaatga cgatgcccac atgtgtaatt
     1261 cgaccgggtg tatcagaggc tgcgaaaaag accaggtcgc cggtcttcag accagaacgc
     1321 ggcacttcgc tgccctgcgc cgccaggtca cgcgcactgc gtgggtactt gctctgcgga
     1381 aactgtgact ctattgcttt gaggacaaaa cccgagcagt caatgccctt gcgcgtcact
     1441 ccaccccagc ggtagggagt acccttccat tcgtcaaaga atgtctcgag cctgggcagg
     1501 ttccaggctg cctgtgcatt ggctgctgca ggttgcacct gctcggcttt gatagtactt
     1561 tcgagctggt cccacagggc atcacctgtg agaggctgca ctgcgccacc gctgcgcgca
     1621 gcagcgccgc ccgaaatttc agccatgaac tgctggatat tgcgctcacc cctcaccttg
     1681 cgcaggcggg gcagttcttc tgcagcgatc gcgattgcat cgttatggcc tttgcccgct
     1741 gaccgtgcct tggcataagt ggcgctgtag gcctcagtct ggtcggccag caggccggaa
     1801 ttctgcgcct gcaaaaagac ctcggcaatt tcttcatcgg atgcgccagc cttctgcatg
     1861 tgttcataga catgccccag tctcacataa ctttctttgg cgatcgcgcg ccagctcagc
     1921 acctcggtaa gatctgaaat ggtggatgct ttgagtccct ggttgagccc gtagtggccc
     1981 agctgcaggg ccgtctgcaa ctgccagtcc tgcagttcaa gataacaggc gcgcggcaca
     2041 accgccgcca gcgtagcagc agggtctgcc ttcttttctg gcagcacccg cgcaaaccgc
     2101 ggtaccgatg tcgcatacga tttcgccacc gattcgggac agtcagccag caggcgcggt
     2161 atcaggtcat cgggaacctg tgcactggca ttgtgcccgg cgaacagcaa agcagttact
     2221 gcaatcagaa tacgtgatat cgtctgggta gatgtttttt gtttcatggg gactccgctt
     2281 ctggtctgat tatcggtaat tcaatgaaaa atcccgtcaa aactccgggt tcagtgtata
     2341 ctccgatttt gccaccgtgt gcttcgacga tatcacgcgc aagcaacagg gcaagccccc
     2401 gaccaaattc gggtgcctcg ggattttcgg ttgcctcaaa catttggcca agacgctcgg
     2461 gaggaatacc gtaaccggaa ttctccactg ccagccttag attgccgtgg gcatcgcttc
     2521 ccttgaccgc cagtttaccc cactgcggcg tgtaccgtac agcgttactg aaaaaaatcg
     2581 tcatcaccca ggcgatcttg gcaacgtcgc aatgcagcgt cggcagcacg taaggaatgt
     2641 taaattctac ggagatatct ttatcggcaa actggttgcg aaacggtgca taagcgagat
     2701 ccgcgagttt gcccggcgat atagaagtga atctgagttg aagaaatgcc ccgcccgtca
     2761 tataaacacc ctaaaaacaa aacggggcag tctctccgac aaccccgcga taataccact
     2821 gaccggtcgc acgaccgata gcagccggct tatttcggct tattgctatc cgaagccgct
     2881 ggcttgttgc cctcggtttt gctgccctgc aaaggtttgc gatcttcttg cgcaatcttt
     2941 gccgggtctt cacccttcag tttaatgcgg tcttcgtggt catggatgcg gttacgcgag
     3001 tcgttcgcgt ctttccggta atcttcgggg atggcgaagt caaaaattgg atcgagccgt
     3061 ttttctttca tgaaatagtt gtctttgaac ttggtctgca gttcatagtt gtcgaagata
     3121 cggttgagct gcatgaccat aatgccgtta accttcgcct ggcggcagag gtcaataact
     3181 gccttgtagg tattgattcg ctcttttttc attgtcggag gcattgcctt gcccttctca
     3241 agccacttct cgagatccat ggccttctgg cgggcaacct ttgcctggcc taactgcatg
     3301 taaccgtaat cgatgttgtc gatcatggct tttttgtcgt acaggtaatg gatatctttc
     3361 gggtcataga ggcgtttttc cagctgtgtc tgtttcttgt ctgcggaaat gcgtacgcct
     3421 gcttcacgct ctttgccaat cttttgggta gcaaaaggcg ccttgcggtt aaagcggatt
     3481 tcaatttctg tcgcttcttt aaccgactgc tgcagcacga tgttcgtgcg gtctatgtag
     3541 aacatcgaaa cctgctcgta gagcttctcg agggtattct ggacatcgag gaaaagtttg
     3601 tacgattcta cgaaattgtc ttcaaaaaag aagctcaggg ccaccgcata tttctgcttc
     3661 acatcgtcgt aaagtttgac gagttcgggt ttttcagagc caaaattccg cagcggaacg
     3721 tcaaggtaga cgatttgctc gagggcctca gcccgcttgt atttggcttt ttcggcgacg
     3781 ttttccgcat aagacagagg cgctgccgca agaagaatca gaaatgctgt ggttttacgc
     3841 atggatatcc tcattccatt gtcggttttc ccccgcgtca agtaaagtat cttcgggcaa
     3901 aaaccgctct ttgccggatt tgcgcagcct tgggccgccg gcatcggctt gacattgaga
     3961 gtccaaaacc ctgctttctg ccgtgcccag cggccacgac cggaaagtac aggaacgacg
     4021 ggattatttt tgccctcgcc tgaatctcca ggcacgctat accggcctca aaaagagcct
     4081 ggcgctgctc gtgctgctcc ttctgggggt ggtaagtccc ctgacagcac ggtcgtggga
     4141 cgacctcaat gaaggtgaaa cggagatcta tggccgggag cgagaggaaa agctccccct
     4201 gcgtctgttc ttcgtccaac gggaaaaatg gcaggaccac gatgcgctgc acattctatg
     4261 gctctacggc tataaggatt acccacgtta cagcagcagg cggctgctac ccttctacta
     4321 ccggctcgag agccgggtcg ataaccgcag gctgaccttt acgcccatca gcctgacaga
     4381 gacagacggc gaaagcgaga gctccatctg gttctggctt ttttttcgtg gcgaagacag
     4441 ggcggcaaaa aaatcgtatt ctgcactgtt cccgttctac taccgggaaa gcctggctga
     4501 cgcctcgtcg gtctactggc tcaccccgct cttctggtac tcgcgcacga aggcgcccct
     4561 tggcgacggc tattcgtttg gtgcgccggt cattccactg gtaatgctga accgtgaagc
     4621 aacatacagc gacttaaccg tgttctacat ctttcgccag cgcagcggcc agcagacgct
     4681 ttcgtacctg ttgccgctgt ggtactacca tagccagcca cagggttatc tcgtctccgt
     4741 gctaccgccc atcgcgtggt atgaacgctc tggcaacagc tcgtggctgc tggtatttcc
     4801 tctctggtat tcttcgcacc accctggcgg cagcacggtg gcatcaccgc tgtacctgcg
     4861 cagcgaatcg gccgacagcc gcctcaacgc gtttatcccg ctttggtttt cgtattcgcg
     4921 taaaaactac cacctgcata tcaacgcagc cggtatttcg ctcgccgaag aacagttttc
     4981 cgccttgcca ttttccgcga cacttgcagg caaccggctg tacgccgatt gggatttcgg
     5041 ctggttctac agcctttttc acgtatcgag ccggagcagc atagccctgg cagatacggc
     5101 aacagaagaa aataaaatcc ccattgccaa tacaggaatc accgagcggc gcaggagaaa
     5161 ccgcgcggac tcagaatcgt tcttcggcct ctacgcgctt tttggtgcgc ttgcctacga
     5221 acgcgcagac cactaccgcc actttcggct gctaccgctg agctggctaa cgtgggattc
     5281 ccgctcggat acgggagtac agaccgtcat ccccttctat gtccattacc gcgatgcaga
     5341 aagccgctac ctggtgatcg cccccctgct ggtaccgttc tacgcatcac aggaaacaac
     5401 gcagagccgg ctacccgaag ataaccagga gctccccgca atttctgcgc cggcaacggc
     5461 tgccgcgccg gccaccggca aatgccgcag cgacaagcag gcatggctga tgataatgtt
     5521 cattcgcgag tacgactgca gccttgaaga gagagagcag acactgctct ggccggtctt
     5581 gaatcgttat acctcgccgc agcgcggtgg atttcgggtt ttccctgtat tctggagtaa
     5641 atggtggcag acaactgtgg gcgaggagcg ctggaatttt tcgccgctgc actactcgcg
     5701 cagcctgaat gacaactacc gtttgttgac ctggctattt tactacctcc gtaacgaaac
     5761 agcaacctct tatggcacct ggggaatatg gcacatcggc agaaccccgg tcaccagcac
     5821 cacatatttt tttcccgttt ggctgcgcac agcgcaggag ccgggagata gagccacagg
     5881 ccgcgccggt taccatgatt cattgctgac cgtcgcaggc ctctggtggc gctcatcttc
     5941 gacaatgggc agcgaccccg cgcgcacggt gcatgtttcg ccactctacc tgtactttgc
     6001 ctcagggagc ggcacggatt tttattcatg gctctactac aggactgacc acccatcgat
     6061 atcccgccac ggcataccac tgcttttcaa ccataccagc cgcggcgata cgtaccggaa
     6121 tttctacctg ttcccgttct accgctcggt cgagctgctt tccaacgacg aaacagaata
     6181 taccagctgg ctgctgccac tgtggtattc acgcagcgac ggtaagtctg cagaacgttt
     6241 tattgccggc tactggtacg agcgccggcc tggttttgca ctcgataact ttctgttaat
     6301 ggccgggcgc tctgacgatg atgcgtctgg ccggcatagc cgccacgcgc tgctctacac
     6361 tttccgttac gattatactc ccaccgaaag caggctcaac ctgctctatg gtctgctgtt
     6421 cagccgcgag caaaccgagt gggagttcag ctggcacttt gccctcatta ccggttaccg
     6481 gataacgccc gatggcgttt accgcaggca ccacctgctg cctgcctact ggtactcggt
     6541 atcggatgat gagcgtatgc tgcttttgcc tgccttgcta tc
//
LOCUS       DAOQJF010000181         6556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6569_length_6556_cov_4.709429, whole genome shotgun sequence.
ACCESSION   DAOQJF010000181 DAOQJF010000000
VERSION     DAOQJF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6569_length_6556_cov_4.709429"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLF85_10260"
     CDS             complement(<1..934)
                     /locus_tag="PLF85_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE20094.1"
                     /translation="MSFSTERSPYIRDLQNGLLEEGRFLVRRATMQSAKNNKPFLRLM
                     LADASGHMPAILFAAPVELKKTAEIARPGKVIRIQGIVEEFQNVKQIKLMKVEAAAET
                     EELSRFQARTPLSRRQMYTEMRQMIGAIASNELKEVCSLFLRDRAFMRLFVEAPASRQ
                     IHHAYIGGLMEHTLNVMRQVAALAKIYPHADHDLLVAGAFLHDIGKTDEYNFLLSHYE
                     HSEAGRLKGHTLLGYEKPVEKLKLVKMHPLTRAKLEHVLLAHQGRRSWGAIEEPRFLE
                     AYLVHAADAIDSTQFIFHDLQKNAENRNTDSAWSE"
     gene            complement(938..2596)
                     /gene="nadB"
                     /locus_tag="PLF85_10265"
     CDS             complement(938..2596)
                     /gene="nadB"
                     /locus_tag="PLF85_10265"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNE20095.1"
                     /translation="MSANAPAPQFLVIGSGIAGLFFALKAAKHGTVTIVTKDKVDNAA
                     TTYAQGGIAAALGTWDNPEKHLADTLAAGAGLCDEEAVGILVREGVRRVQELIDLGMP
                     FTRGEDGKLHFGREGGHGESRVLHTHDHTGRSLESFLLERVSAEPNIRLLTNHCVVEL
                     ATEHHLPQRSNSTTVFGAYVLDAATNEIDLFHADYTILATGGAGRVYPVTTNPPVSTG
                     DGVALAYRAGCRVRNMEFFQFHPTALALKGKAPPGTPAFLITEAMRGEGAILKNPRTG
                     RAFMQDYHEQRELAPRDIVARAIDAELKRDGLDYVWLDISHRDAAFIKEHFPLIFGTL
                     KEQFNIDITREPIPVVPAVHYMCGGVLVNYDGLTDLANLYAIGEVASSGVHGGNRLAS
                     NSLLEGLVFAQRALDHVLAGKASLDPFRATASRLTEWDHTNTKNPEEWVLISHDFKEI
                     RSIMWDYVGIVRSNLRLERALRRVDVIYGEIIDFYNRTVISRDLLELRNLALVAQLTI
                     RSALARKESRGLHFTTDYPEHREPSRNDTILSPAHFNPKARQFT"
     gene            complement(2692..3435)
                     /locus_tag="PLF85_10270"
     CDS             complement(2692..3435)
                     /locus_tag="PLF85_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNE20096.1"
                     /translation="MRYLQHVEKFVDGMKVVEIPGASTSLSNPGYTFFCMHGYGANAY
                     DLAGLSQYLNLPEGTRFIFPDGILKIDLGYAAGYEGRAWFPIDWAEIERIRREGGLRD
                     LSKTLPAGMAEARDRIIEAMQKLECDPARTVIGGFSQGSMLATEIALHHKEQFAGLLI
                     FSGTLVNRTGWENMAKAKFADQQKTLRFHQSHGRLDPVLPFQAAERLYELLTTNGAQG
                     ELHAFEGQHEIPAAVVETTNRWLGSLFSP"
     gene            complement(3962..4390)
                     /locus_tag="PLF85_10275"
     CDS             complement(3962..4390)
                     /locus_tag="PLF85_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002123589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20097.1"
                     /translation="MEDFKMDVKLTPGENIVFKIEADFWQRGNNPLQKIVGNFMRIFS
                     KILGYRVNGALIVTNKRVLELTEEYRCWCINTNRGVKVLTRRGVKEFGYEMEKTCGIF
                     CPTYYLYYEGDTQSTLIAVQDGSDQKMMEILEKFQAALEG"
     gene            4612..5181
                     /locus_tag="PLF85_10280"
     CDS             4612..5181
                     /locus_tag="PLF85_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20098.1"
                     /translation="MEKILILRPLLKRISEGSIFRVLFAWAMRIEAALTVLGVIYGSV
                     KLWGLVRGGGAPASAYFGLLIVQIALLILGLVIVNILLVRAEDIARQPVGSDYIVTPI
                     IVIFQKTMGEIGAAVYGVLGVAGGLFIWIAGGEIPLPIPGLSGGDGFSGGLIAMIMGL
                     TIGFGVLAFAYFMAELTGAVVDIARNTKK"
     gene            complement(5214..5696)
                     /locus_tag="PLF85_10285"
     CDS             complement(5214..5696)
                     /locus_tag="PLF85_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HNE20099.1"
                     /translation="MRKLEQHVFPRWQQLSEDLGFDAAFSQFGPYSGANITVRTIDRY
                     TVESSMTLVESNTNYVGTHFGGSLYSMCDPFFMFLLMHNLGEGYIVWDKAATIDFIKP
                     GTGTVTAHFHIPEAEFVNLRSELGKTKKLDRIYVCEVTDEKGDVVARMSKTLYIRRMR
                     "
     gene            5817..6434
                     /locus_tag="PLF85_10290"
     CDS             5817..6434
                     /locus_tag="PLF85_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20100.1"
                     /translation="MRRSLFVYCLFVLVLAQQGLFALQGESIFLKGYLGGGYSYVTQK
                     DEKAGTGLTLNGPIGMSMLQAGGSLNASTKLFAFTSFHYGPSLSATAENLKIDTQYSY
                     FFVHDFGLGIGFYSKGGLSLSLGGAISNNYYNYSVYQTTGVGTHTRHGWAAHAIVGQE
                     LSTTGRFILGVSLLLSYNRVYDVGPSSDAAVTGIYGGLAVSAMYD"
BASE COUNT         1430 a         1687 c         1797 g         1642 t
ORIGIN      
        1 attctgacca ggctgagtcc gtgttcctgt tttcggcgtt cttttgcagg tcgtgaaaga
       61 taaattgtgt tgaatcgatt gcgtcggcgg cgtgcaccag gtacgcctcg agaaagcgcg
      121 gttcttcaat cgctccccag ctgcgccggc cctggtgggc gagcagaacg tgttcgagct
      181 tcgcgcgcgt cagcgggtgc atcttgacga gcttcagctt ctctaccggt ttttcgtaac
      241 ccagcagcgt gtgccccttc agccgaccgg cttcggaatg ttcgtaatgg ctcaggagaa
      301 aattgtattc gtcggtttta ccgatgtcat gcaggaaagc tcctgcgacc agcaggtcat
      361 ggtctgcgtg cgggtaaatt tttgccagcg ctgctacctg gcgcatgaca ttgagtgtgt
      421 gctccatcag gccaccgata taggcatggt gtatctggcg cgaagccggg gcctcgacaa
      481 acaggcgcat gaatgcccgg tcgcgcagga acaggctgca gacttctttg agttcgttcg
      541 aggcgatcgc cccgatcatc tgccgcatct cggtgtacat ctgccgccgg ctgagtggcg
      601 ttcgtgcctg gaagcggctg agctcctctg tctcggcagc cgcctcgacc ttcatgagtt
      661 tgatctgctt gacgttctgg aattcttcaa cgataccctg gatacggata acctttccag
      721 gacgcgcgat ttctgcagtc ttcttcagtt cgacaggtgc cgcaaaaagt atcgcaggca
      781 tgtggcctga ggcatcggcg agcatcaggc gcaggaaggg tttgttgttc ttggcgctct
      841 gcatggtcgc ccggcgtacc aaaaagcggc cttcttcgag caggccgttc tgcaggtcac
      901 ggatataggg cgaacgttct gtggagaaac tcatcggtca ggtgaattgc cgggccttcg
      961 ggttaaagtg cgccgggctc aggatcgtgt cgttgcgtga tggttcgcgg tgttccgggt
     1021 aatcggttgt gaagtgcagg ccgcggcttt ctttgcgcgc gagcgccgaa cgaatcgtca
     1081 gctgcgcgac gagcgccagg ttgcggagtt caagcaggtc gcgtgaaatg accgtccggt
     1141 tgtagaaatc gatgatctcg ccataaatga catccacacg gcggagcgca cgttccaggc
     1201 gcaggttcga ccgcacgata ccgacataat cccacataat ggaacggatt tctttgaagt
     1261 catggctgat caacacccac tcttcagggt tcttggtgtt cgtatgatcc cattctgtca
     1321 ggcggctggc tgtagcacgg aaagggtcga gcgatgcctt gcctgcaagt acatggtcga
     1381 gcgcacgttg tgcaaaaacg agcccctcaa gcagcgaatt ggatgcgaga cggttgccgc
     1441 cgtgtacccc tgacgaagcg acttcaccga tcgcgtagag gttcgcgagg tcggtaagcc
     1501 catcgtagtt caccagtact ccgccgcaca tgtagtgtac tgcgggtaca acgggtatag
     1561 gctcgcgagt gatatcgata ttaaactgct ctttgagagt gccaaagatc agcgggaagt
     1621 gctctttaat gaatgcggcg tcgcggtggg agatatcgag ccagacgtaa tcgagaccgt
     1681 cacgtttaag ctccgcatcg attgcccggg caacaatgtc gcgcggtgca agttcacgct
     1741 gctcgtggta gtcctgcatg aaggcacgcc cggtacgcgg gtttttgagt attgcgcctt
     1801 ctccccgcat cgcttcggtg atgagaaaag cgggtgttcc cgggggagct ttgcccttga
     1861 gtgccagtgc tgtcgggtga aactggaaaa actccatgtt gcgcacgcgg cagcctgcgc
     1921 ggtatgccag cgctacacca tcgccggtcg atacaggtgg gtttgttgtt acagggtaaa
     1981 cacggccggc accgccggtt gcgagaatag tatagtctgc gtgaaaaaga tcaatctcgt
     2041 tggtggccgc atcgagcaca taggctccaa acacggtcgt ggagttgctg cgctgcggca
     2101 ggtggtgttc ggtcgccagc tctaccacgc agtggttggt cagcaggcgg atattgggtt
     2161 cggccgaaac gcgctcaaga agaaacgatt caagtgaacg tcccgtgtga tcatgagtgt
     2221 gcagcacgcg cgattcgccg tggccacctt cgcggccgaa atgcagcttg ccatcttcac
     2281 cgcgtgtgaa cggcattccc aggtcgatga gctcctgtac acggcgcact ccctcgcgta
     2341 ccaggatgcc gactgcttct tcgtcgcaca ggccagcgcc tgccgccagt gtatcggcga
     2401 gatgcttttc cgggttatcc caagtgccga gcgcggcagc gatgccaccc tgcgcatagg
     2461 tcgtggccgc attgtcgacc ttgtcctttg tcacgatggt tacagtgccg tgctttgcgg
     2521 ctttgagtgc gaagaagagg ccggcgatgc ccgagccaat gaccagaaat tgcggggcgg
     2581 gagcgtttgc actcacgaat cacaactcct gaaggtcaaa tagcccgacc acagatttaa
     2641 cagaatgaga ctatagtgga aaaccacgcc ctgtcgccgt ggttaatgga cttatggtga
     2701 aaacagactg cccagccagc ggttggttgt ctcaacgacc gcggcgggaa tttcgtgctg
     2761 gccttcgaaa gcgtgcagtt caccctgagc accattggtt gtcagcagtt cataaagccg
     2821 ttcggccgcc tggaacggca ataccgggtc gagcctgcca tgcgactggt gaaaacgcag
     2881 cgtcttctgc tggtcggcaa atttcgcttt tgccatgttc tcccagccgg tgcggtttac
     2941 cagagtacca gaaaatatca gcaggcctgc gaattgctct ttgtgatgga gcgcaatttc
     3001 tgtcgcgagc atcgagccct gtgaaaaacc gccgatcact gttctcgccg ggtcacactc
     3061 gagtttctgc atcgcttcga tgatgcggtc acgcgcctca gccataccag cgggcaaagt
     3121 tttggacaaa tcgcgtaatc caccttcgcg gcggatacgc tcgatctctg cccagtcgat
     3181 cgggaaccag gcgcggcctt cgtaacccgc agcataaccc aggtcaattt tgagaattcc
     3241 gtccggaaaa ataaaccgtg taccttctgg cagattgagg tactgtgaca ggcctgcgag
     3301 gtcataggca ttggcgccat agccatgcat gcagaagaag gtatatcccg ggttgctgag
     3361 cgaagtcgaa gcacccggta tctcgacgac tttcatgcca tcgacgaact tctctacgtg
     3421 ttgcaggtag cgcatggggg ctattgcggc aaggcgccac acgggtaaat gaataagtag
     3481 tttgttttcg ttacataccg gtgacggagc agatgagcaa aaaacctcga ctacctaaag
     3541 ctctgttatc gtcgtcttgt tatctttcac aaaagtaacg cgtgttcgtt gcctggtccg
     3601 aaaggcaaga aacgatgttt ggtatttcct tcgtaactct gagtaccgat tgggttggcc
     3661 aattttctcc aaagctattc gcgctacctg acggccacaa ggtgcattca tataatcggc
     3721 aaaccgctcc gggcgaaaat ttcttcgatt cgtaacgacc aagcggctga tcgctcgcag
     3781 tttatgagga atcgttccga gcaaaatgac tacctgatta atagttagta gatcgatgag
     3841 gacgagcacg attgtaacaa tcagccaatg ctcagcataa aaccaaaggg caagaaaatt
     3901 tacgatcgac aaagtcacca gaatcgcaat cggatacttt gccaatcgta aagtgagcaa
     3961 attagccttc tagagccgcc tgaaatttct caagaatctc catcattttc tggtcagagc
     4021 catcttgtac ggcaatgaga gttgattgcg tatcaccctc gtaatacaga tagtatgtag
     4081 ggcagaatat gccgcaagtc ttctccattt cgtacccaaa ctctttgacg ccgcgccgtg
     4141 tgagaacctt tacgccgcga ttggtattga tgcaccaaca ccgatactct tcagtcaatt
     4201 cgagcactct cttgttcgtc acgatcagag caccatttac acgataaccg agaatctttg
     4261 agaagatgcg catgaagttt ccaacgatct tttgcagagg gttatttccc ctttgccaga
     4321 aatcggcttc tatcttgaat actatgttct ctcccggagt caattttaca tccatcttga
     4381 aatcctccaa actctaattc cagatcctca gatctggacg gcgaccttgg gctaactgga
     4441 caattcggat agtttttttt actgaataga tgttgctgtg cacaaatgcc tcactttctt
     4501 ttaggggctg tgatttgata ttttgcttaa ttcatttgct ccaatatttc gttgacgcgc
     4561 tccgagatac ctgcgaactc ttgtataaat cgagagaatg gaggaaatta aatggaaaaa
     4621 attcttatac tgcgaccgct tctcaagcgc atatccgaag gcagcatttt tcgtgtgctc
     4681 tttgcctggg ctatgcgcat agaggcggct ctaacggtac tcggggtaat atacggctcg
     4741 gtgaagttgt ggggcctggt caggggcggc ggagcaccag ccagcgccta ttttggcttg
     4801 ttgattgtgc aaattgcgct acttatcctg gggcttgtca tagtgaatat tctcctcgtg
     4861 cgcgctgaag atattgcgcg acagccggta ggttctgact acatcgtgac ccccattatc
     4921 gtgattttcc agaagacaat gggtgaaatc ggcgctgcgg tttatggtgt tcttggcgtt
     4981 gcgggcggtc ttttcatctg gatcgctggt ggtgaaattc cgttaccgat tcctggtctt
     5041 tccggtggtg acggtttttc tggcgggtta attgccatga taatgggctt aaccattggt
     5101 tttggagttc ttgcgtttgc ctattttatg gcggagctca caggtgctgt cgtagatatt
     5161 gcgcgaaata caaaaaaatg atttcaaggg ggcgaattct tcgccctttt ttatcaccgc
     5221 atccttctga tatagagggt cttgctcatt ctcgcgacca cgtcgccttt ttcgtccgtc
     5281 acttcgcaga catagattcg atcgagtttt ttggttttcc cgagttctga ccgtaaatta
     5341 acgaactccg cctcaggaat atggaagtgt gccgtgacgg tacctgttcc aggttttatg
     5401 aagtcaatcg tcgccgcctt gtcccagacg atataacctt cgcccaggtt gtgcatcagt
     5461 aaaaacataa agaacgggtc gcacatcgaa tagagcgagc cgccgaagtg cgtgcccaca
     5521 tagttggtat tgctttcgac cagcgtcatg gacgattcga cggtatagcg gtcgattgta
     5581 cgcacagtga tatttgcgcc ggaatagggg ccaaactgcg aaaaagcggc gtcaaagccc
     5641 aggtcttcac tcaactgttg ccagcggggg aatacgtgct gttcaagctt gcgcatagcc
     5701 tgacctagga aaaatcctat acgccccgac aaccaattgg ggcattgcaa aaagaaaacg
     5761 acaccagtgt cgtgaagttt tggccccttg cgtcgataga gtatatagag aagtaaatgc
     5821 ggcgttcact attcgtttac tgtttgtttg ttctggttct tgcccaacag ggcctgtttg
     5881 cgctccaggg tgagagtatt ttcctcaaag gctacctggg tggtgggtat tcgtatgtaa
     5941 cccagaaaga tgaaaaggcg ggtacgggcc tgaccttaaa tggtccgatc ggtatgagca
     6001 tgctgcaagc gggtggttcg ctcaacgcct cgaccaaatt gttcgcgttt acttcgttcc
     6061 attacgggcc atcgctgtcg gctacagcag aaaatctcaa gatcgacaca caatacagct
     6121 acttctttgt gcatgacttt ggtttaggaa tcggctttta ttcgaagggc ggactctcgc
     6181 tctcgttggg gggagctatc tcgaataact actacaacta cagcgtgtac cagaccaccg
     6241 gcgtgggcac ccatacgcgc cacggctggg ccgcgcacgc gattgtcggc caggaactca
     6301 gcaccacggg acgttttatt ctcggcgtat cgcttttgct ttcctacaac cgggtctatg
     6361 atgtgggacc gtcgtctgac gccgctgtaa ccggtattta cggcggcctt gccgtttcgg
     6421 ccatgtacga ttgacgggcc catagctccg caataattct cgccacccgc tgcggttttc
     6481 tatggctggg cgatagtcgc ccatggaagc taaaaccacc gaactcaaac gcagtcttgg
     6541 tcctctttcg gtctgg
//
LOCUS       DAOQJF010000182         6553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6571_length_6553_cov_4.814404, whole genome shotgun sequence.
ACCESSION   DAOQJF010000182 DAOQJF010000000
VERSION     DAOQJF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6571_length_6553_cov_4.814404"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLF85_10295"
     CDS             <1..423
                     /locus_tag="PLF85_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNE20101.1"
                     /translation="LEALQFMTNLIVEKVTGEQPKILLDIENYRERRAQHLMEIAKKT
                     GEYVARTGKSRLLDPLNPYERRLVHMALQDNETVKTESEGNGVYKRVRVFRVSNGQPA
                     EPGNELVTEENIGNINFEAGQDPEGAEDIEFKSDDFNR"
     gene            392..1786
                     /gene="mnmE"
                     /locus_tag="PLF85_10300"
     CDS             392..1786
                     /gene="mnmE"
                     /locus_tag="PLF85_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNE20102.1"
                     /translation="MSSRATISTDNPICAPATAAGARSAIAVIRASGSPGSGRNIFDR
                     LGNSFTTKTNKVAAQLSAREAHYGILVDGNERIDDVIFTRFDGRASFTGEDSFEISCH
                     GNPLIIRRILALLYSRGFRAAEPGEFTRRAYLNGKLDLDAAQAVAEIIEARSAVSLKA
                     AQRLAQGSFRRDMLQLRSQLMNLLADLNAELDFIDEDISFATRETKLRILHETAEKAL
                     YLETEAARFETMKNGFHIAIVGAPNAGKSSLMNRFIGHDRSIVSDIAGTTRDYIEAEM
                     EIAGVNIRLFDTAGLRDETGDAIEQIGMERTLELMNRAHICILLVDGSEAAGTDSFSA
                     VLASEATRLTVVNKADVLHPSWQNTAAERSTLFISARTGQGIDELLSAIGGIIEEKTP
                     PDALPLSIWQRRLLNEIAGLAKSAAAAVNENEMPEVITHTVNQAVDRIAELTGEITSE
                     DILGRIFSRFCIGK"
     gene            complement(1813..2490)
                     /locus_tag="PLF85_10305"
     CDS             complement(1813..2490)
                     /locus_tag="PLF85_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNE20103.1"
                     /translation="MSLQENYQHVREQIANACVRSGRPPESVSLIAVSKTHPASYVDQ
                     VAALGQVHFAENRIAELVEKKASVQAPALQWHLIGQLQTNKVKLLEADTILHTLDRAS
                     LADKLQSRFTDATISCLLQVNCSGEPSKSGVAPAEFNALADYVAQKPAIRILGLMTMA
                     ENTEDEWRIREAFAKLRQLSEGLQARRIFPGYKGWLSMGMSGDFELAIAEGATHVRVG
                     SAIFGSR"
     gene            complement(2487..3977)
                     /locus_tag="PLF85_10310"
     CDS             complement(2487..3977)
                     /locus_tag="PLF85_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNE20104.1"
                     /translation="MRCVQQGIALLVFATVCACNAGSNGEPPNQGALQQTAAGQIVAN
                     LSNRGISKDPRFTDTSMIALYLYKKVDYSLSCASASDRLRCEDALVFKNEKVDSLGFT
                     NEPGYFGYDATYSSYFPSLVADPGQNYPSVKPADPPGFADPDVWQCIRNPFTTSPLYN
                     KFECGVNSHPIRYFRRDLTTYTLQVYATNGQGFASSYILDYNLFNSLNHPVKDGDRYY
                     AYFAVHYNAMRSASDSEDRFDATATTWKRPIEPDLVSPRIEVRVKLLAEGPYDKAGSV
                     YDIPDYSTWYDPLAGCRPSQSVPAGSVYISEILWMGSEDTAGAAESDDEFIEIYNSNT
                     FDVVLSGWKINGAGSGTSALALPTCAVIKAGGVYTVGRQTTKAFSTFDFTTNLLSLSN
                     SGESSLGLVDAAGNTISSVTGCTTGPWGNFGINGGTGIPKRSMRLTNIATRADTCAAG
                     WSATSRGDAGYGGDIANVRTSYAYPPTQPDDASGEGTTATPGYAGP"
     gene            4068..4493
                     /gene="yajC"
                     /locus_tag="PLF85_10315"
     CDS             4068..4493
                     /gene="yajC"
                     /locus_tag="PLF85_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNE20105.1"
                     /translation="MNEVLYTVAQAAPVPVTTPGAAPAPAAAGATATVAPAGKPAAAP
                     QQPDMSSALSSFALPVLMIVIFYFLLIRPQQKREKERQKLLKGIEKGDRVLTRGGIFG
                     TVVGVKQEENIAIVKIADEVKVEVAISTLETVVNKTAGK"
     gene            4499..5050
                     /locus_tag="PLF85_10320"
     CDS             4499..5050
                     /locus_tag="PLF85_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20106.1"
                     /translation="MRRLFGFFTLSVLLANALPALQAPADCGQCIAQNIFDEDTGANS
                     EVAEKPASNEETGKSKSTGKRKKKKKSRKKKKQAAQPAPSVSESQEFTPQELARQSYA
                     WAPEPGLRLKSTAPGIKPAETAVPAKAAAPAPAEVLETPVQPQGFKLPQIPVTQVLIV
                     AGFVILFLIYRFRVGRQMKRRKY"
     gene            5078..>6553
                     /gene="secD"
                     /locus_tag="PLF85_10325"
     CDS             5078..>6553
                     /gene="secD"
                     /locus_tag="PLF85_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNE20107.1"
                     /translation="MSAQVRFFLIISVVGLAIALIWPNIGERTIRLYAVESLEAAKRE
                     EALARAEKYVKDNYSVKYHTERKSGKPIGNPKAPDEAYVSITGKFVQTAFLNELGRLE
                     GVDPDRITLEPMWVEENLKAKAFKLGLDLQGGMNLVLEGDFKKLEETLRRQYPPELVE
                     SLKKQLEAEKDADKKKTLQAKLDEIKNAFDFSPQRKKQDVEGALEIMRSRIDARGVSE
                     PLIRMQGEERIEISLPGVANPDQAKKLIASTARVAYHLHDPAGEAGRLTQEANREFAE
                     YLKQSTDFQKQEFLRKLEAKLKFPKNLGIYLSYDKRPNARGVNELQPREFLVLNNEPV
                     LTGDAMSRTVYATVDPEKGQNIVSFELTSAGTDTFAKITTENKGKLLAILIDNKIRSA
                     PQINEPILGGRGQISGNFSAQEATDLALIIKEGALPVPMTIVEERSVGPTLGQEAIDA
                     GLKAIVVGFLLVVLYMVLYYHGSGVIASFALLINLLLVSAIF"
BASE COUNT         1604 a         1857 c         1774 g         1318 t
ORIGIN      
        1 ctcgaagcgc tgcagttcat gacaaacctg attgtcgaaa aagtcacagg agagcagccc
       61 aaaattctgc tcgatattga aaattaccgt gaacgccgcg cgcagcacct gatggaaatc
      121 gctaagaaaa ccggtgaata cgtggcgcgc accggcaaaa gccgactgct cgatccactg
      181 aacccgtatg agcgccggct ggtacacatg gcgctgcagg ataacgaaac agtaaaaacc
      241 gaatcagagg gtaacggagt ctacaagcgc gtacgcgtat tccgcgtcag caacggtcag
      301 cctgctgaac ctggaaatga actcgtaacc gaagaaaata tcggtaatat caacttcgaa
      361 gctggtcagg accctgaagg cgccgaagac attgagttca agagcgacga tttcaacaga
      421 taacccgatc tgtgctcccg caaccgctgc gggagcgcgt tctgccatag cggtcatccg
      481 cgcgagcggc tcaccgggtt caggccgcaa catctttgac cggctcggca acagtttcac
      541 cactaaaaca aataaggtcg cggcgcagct tagtgcgcgc gaagcccact atggcatact
      601 cgtcgatggc aatgaacgta ttgacgacgt cattttcacg cgctttgacg ggcgcgcctc
      661 gtttaccggc gaagacagct tcgagatcag ctgccacggc aacccgctga ttatcaggcg
      721 catactcgca ctgctttatt cgcgcgggtt tcgcgccgcc gaacccggcg aattcacgcg
      781 gagagcctac ctgaacggca agctcgacct ggatgccgcg caggccgtag ccgaaattat
      841 cgaagcgcgc agcgcagtct cgctcaaggc tgcgcagcgc cttgcgcagg gcagttttcg
      901 gcgggatatg ctgcaactac gttcgcagct gatgaacctg ctcgccgacc tgaacgcaga
      961 gctcgacttt atcgacgaag acatcagctt tgcgacgcgt gaaaccaagc tgcgcattct
     1021 gcatgaaaca gcagaaaaag ccctttatct cgaaaccgaa gcagcacgct ttgaaaccat
     1081 gaagaacggt ttccatattg cgatcgtagg agctcccaac gcgggcaaat cgtcgctgat
     1141 gaaccgcttc atcggccatg accgctcgat cgtcagcgac atcgccggaa ccacgcgcga
     1201 ctatatcgag gctgaaatgg aaatcgcggg tgttaacata cgcctgttcg ataccgccgg
     1261 gctgcgagac gaaaccggag atgcgatcga acagatcggt atggaacgta cgcttgaact
     1321 gatgaaccgt gcccatattt gtattctgct cgtcgacggt tcagaagcag ccggcacaga
     1381 ttcattttct gcggtattgg ccagtgaggc aacgcgcctg acagtcgtaa acaaagcaga
     1441 cgttctgcac cccagctggc agaatactgc agcagagcgc agcaccctct ttatttctgc
     1501 ccgcaccggt cagggcatcg acgaactgct cagcgcaatt ggcggcatca tcgaagagaa
     1561 aactccgccg gatgcgctgc ccctgtcaat ctggcagcgt cgcctgctga acgaaattgc
     1621 cgggttagcc aaaagcgcag cagctgcggt gaacgaaaat gaaatgccgg aagttatcac
     1681 tcatactgtg aaccaggcgg tagatcgaat cgctgagcta accggggaaa tcacaagcga
     1741 agatattctg ggcaggatct tcagccgttt ctgtatcggt aagtaggggg taggttaaaa
     1801 cctaccccct acttaccgac tcccgaaaat tgcgctcccg acgcgcacgt gggtggcccc
     1861 ttcggcaatt gcgagttcaa aatcgcctga catgcccatg gacaaccagc ccttatatcc
     1921 gggaaaaatc cgccgagcct gaagcccttc agagagctgc cggagtttgg caaacgcctc
     1981 ccggatacgc cactcgtctt cggtattttc agccatggtc atcagcccca ggatgcggat
     2041 cgcaggcttc tgcgcaacat agtccgccag cgcgttaaat tctgccgggg caacaccgga
     2101 cttactgggt tcaccgctac aattcacctg tagcaggcaa ctgatcgtgg catcggtgaa
     2161 acgcgattgc agcttatccg ccagcgaggc ccgatcgagc gtatgcagaa tggtatctgc
     2221 ttcgagcagc ttgaccttgt ttgtctgcag ttgaccaatc aggtgccact gcagcgccgg
     2281 ggcctgaacc gaagccttct tttctaccag ttcggcaatg cggttctcgg cgaaatgcac
     2341 ctgtcccagg gcggcaacct ggtccacgta cgatgccggg tgcgttttgg aaacagcaat
     2401 caggctgacg gattcgggag gtctgccact gcgcacgcag gcgttggcaa tctgctcgcg
     2461 gacatgctgg tagttctcct gcaaactcac gggccagcgt atccaggggt ggctgtggtg
     2521 ccttcgcccg acgcatcatc gggttgcgtc gggggatatg cgtaggatgt acggacattg
     2581 gcaatgtctc cgccgtagcc agcgtcgccg cggctcgtcg cactccagcc cgccgcacag
     2641 gtatctgcac gcgtggcgat attcgtcagc cgcatagaac gtttggggat gcctgtcccc
     2701 ccgttgatac caaaattgcc ccacggaccg gtggtgcaac ccgtaacgct gctgatggtg
     2761 ttgccggccg catcgaccag gcctaaggaa ctctcacctg aattcgagag ggagagcaag
     2821 tttgtggtga aatcgaaggt actgaaggcc ttagtcgtct gtcgcccaac ggtgtagaca
     2881 ccgcccgcct tgatcaccgc acaggtgggc agcgccagcg ccgacgtacc cgaaccggcg
     2941 ccgttgatct tccagcccga gagcaccaca tcaaaggtgt tgctgttata gatttcgata
     3001 aattcgtcgt cggattcagc agcgccagcg gtatcttcgc tgcccatcca caggatttcg
     3061 ctgatataga ccgacccggc gggtaccgat tgcgaaggcc ggcaacccgc cagcggatca
     3121 taccaggtgc tgtagtcagg tatgtcgtag acgcttcccg ccttgtcgta agggccttcg
     3181 gcgagcaatt ttaccctgac ctcgatgcgt ggcgaaacca ggtcgggttc aatcggacgc
     3241 ttccaggtgg tggctgtggc atcaaagcgg tcttcggagt cactcgctga gcgcatagca
     3301 ttgtagtgaa cggcaaaata ggcgtagtag cgatcgccat cctttaccgg gtggttgagg
     3361 ctgttaaaca ggttatagtc gagtatatag gacgaggcaa agccctgccc gttggttgca
     3421 tagacctgca gcgtgtaggt ggtcagatcg cgccggaagt acctgatcgg atgcgagttt
     3481 actccgcatt cgaatttgtt gtacagcggg cttgtggtga acggattgcg aatacactgc
     3541 cagacatccg ggtcagcaaa acccggcggg tcggcaggct tcaccgaagg ataattctgt
     3601 ccggggtcag ccacgagcga aggaaaatac gagctgtagg tcgcatcata gccaaaatat
     3661 ccaggctcgt tggtgaatcc cagcgaatcg accttttcgt ttttgaacac cagcgcatcc
     3721 tcgcagcgca gccgatcgct cgcgctggcg cagctcagac tataatcaac tttcttgtag
     3781 agatagagag caatcatgct ggtgtcagtg aaacggggat ctttgctgat accccggttc
     3841 gacaggtttg ccactatctg accggccgca gtctgctgta gcgcaccctg gttgggtggt
     3901 tctccgttcg aaccggcgtt gcacgcacag acagtcgcaa acaccagcaa cgcgataccc
     3961 tgttgaacac agcgcacacc gcacaaggcc tcagcctcct gcgccggcaa ctattttcct
     4021 tgcctgccat tcattgacgc ggtgtgtcaa aaacctgccc tgcctgtatg aatgaagttc
     4081 tctatactgt agcccaggct gcacccgtcc ccgtcaccac cccaggagca gctccggcac
     4141 ccgcagcagc tggtgcaacc gctactgtgg ctccagcagg caaacctgca gcagccccgc
     4201 aacagcccga catgagcagc gcgctttcaa gctttgcgct gcccgtgctg atgatcgtga
     4261 tcttctattt cctcctgatt cgcccacaac agaagcgaga aaaagaacgc cagaaactgc
     4321 tcaaaggtat tgaaaaaggc gaccgtgttc ttacccgcgg tggtattttt ggcacagttg
     4381 tcggagttaa acaggaagaa aacattgcca tcgtcaaaat cgccgacgaa gtcaaagttg
     4441 aagtcgcgat ttcaactctt gagacagtag tcaacaagac cgcgggtaaa taattcccgt
     4501 gcggcgcctg ttcggcttct tcaccttgtc tgtgctgctg gcaaacgcgt tgccagcgct
     4561 gcaggctccc gcagattgcg ggcaatgtat tgcgcaaaac atcttcgacg aagatactgg
     4621 cgcaaacagt gaagttgctg aaaaacccgc ctcaaatgaa gagaccggga aaagtaaaag
     4681 caccggcaag cggaagaaaa agaagaaatc ccgcaagaaa aagaaacaag ctgcccagcc
     4741 ggcaccgagc gtatctgaaa gccaggaatt caccccgcag gaactagcga ggcagagcta
     4801 cgcatgggca cctgaaccag gccttcgcct caagagcact gctcccggta taaaacccgc
     4861 cgagacagct gtaccggcca aagccgcagc cccggcacca gccgaggtgc ttgagacccc
     4921 ggtacaaccg cagggtttca aactgccaca gataccggtt acgcaggttt taattgtcgc
     4981 cgggttcgtc attttgtttc tgatctaccg cttccgcgtc ggcagacaga tgaaacgcag
     5041 gaaatactaa taaaccaatt acccgagagg aaaaccagtg agcgcacaag tccgtttttt
     5101 cctgattatc tccgtcgtgg gcctggccat tgccctgatc tggcccaata tcggtgagcg
     5161 tacgatcagg ctttatgccg ttgagtcgct agaggccgcc aaacgtgaag aagccctggc
     5221 acgggctgaa aagtacgtta aagacaacta cagtgtaaaa taccacactg agcgcaaatc
     5281 cggtaagcca atcggcaacc caaaagcacc ggacgaagct tatgtgagta tcactggcaa
     5341 gtttgtccag actgcatttc ttaatgaact gggccgcctt gaaggcgtag acccggaccg
     5401 catcacactc gaacccatgt gggttgaaga aaacctgaaa gccaaggcat tcaagctagg
     5461 tctcgacctc cagggcggta tgaacctcgt cctcgaaggg gatttcaaaa agctcgaaga
     5521 gacgctccgc cggcaatacc cacccgaact ggtggaaagc ctgaaaaagc agcttgaagc
     5581 agaaaaggat gcagacaaga agaagacact gcaggcgaaa ctcgacgaga tcaagaatgc
     5641 tttcgatttt tcgccgcaac gcaaaaagca ggatgttgag ggcgcgctcg aaatcatgcg
     5701 ctcacgtatc gatgcgcgtg gcgtttcaga acccctgata cgcatgcagg gcgaagagcg
     5761 catcgaaatt tcgctcccgg gcgttgctaa ccctgaccag gccaaaaaac tgattgcatc
     5821 gactgcccgc gtcgcctacc acctgcacga tcccgcgggt gaagccggtc gcctgacgca
     5881 ggaagccaac cgggaatttg cggagtacct gaagcagagc acagacttcc aaaaacagga
     5941 attcctccgc aagcttgaag ccaaactgaa attcccgaag aatctcggta tctacctgtc
     6001 gtatgacaag cggcccaatg cgcgcggcgt caatgaactg caaccgcgtg aattccttgt
     6061 actgaataac gaaccggttc tgaccggtga tgcgatgagc cgtactgtct atgcgactgt
     6121 tgacccggaa aaagggcaga acatcgtgag ctttgaatta acctctgcgg gtaccgacac
     6181 ctttgcgaaa atcaccacag aaaacaaggg taagctgctt gccattctga tcgacaacaa
     6241 gatccgctcg gctccacaga taaacgagcc cattctcggc ggtcgtggcc agatcagcgg
     6301 taatttcagc gctcaggaag cgaccgatct tgccctgatt attaaagagg gagctctgcc
     6361 tgttccaatg actattgtcg aagaacggtc ggttggccct actcttgggc aggaagccat
     6421 tgatgccggt ctgaaagcaa tcgttgtcgg ctttctgctc gtcgttctct acatggtgct
     6481 ctattaccac ggatcagggg taattgccag cttcgcgctg ctcatcaacc tgctgctggt
     6541 ctcggccata ttt
//
LOCUS       DAOQJF010000183         6536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6597_length_6536_cov_4.755902, whole genome shotgun sequence.
ACCESSION   DAOQJF010000183 DAOQJF010000000
VERSION     DAOQJF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_6536_cov_4.755902"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLF85_10330"
     CDS             complement(<1..261)
                     /locus_tag="PLF85_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20108.1"
                     /translation="MVSRILITILLLPALLPAAGVRTVGRVERIDPVTHIAEISAAAA
                     LEPGKEYTVLNEAGEVIGSAVDVRRIGNGNSVFTFAGKRRSLS"
     gene            385..639
                     /locus_tag="PLF85_10335"
     CDS             385..639
                     /locus_tag="PLF85_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNE20109.1"
                     /translation="MKNPQLVHRLSRIQGQIEALKRIAASEEADCLKTIELAKAASNA
                     IKKFAQAYVEEHLEQCVEEKRALKEIQTELRKVVQSSFSL"
     gene            complement(679..1866)
                     /gene="argJ"
                     /locus_tag="PLF85_10340"
     CDS             complement(679..1866)
                     /gene="argJ"
                     /locus_tag="PLF85_10340"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNE20110.1"
                     /translation="MAIENRSISESFKSKPLTAAPRGFRSFITNAGIKDTSLDLGVVV
                     SEVPATAAAIFTKNQVKGNPVQVGMKHIRGGKVTALIVNSKNSNVATGKPGYADCVQV
                     CKAVADELKIPVTQVFPSSTGVIGRRLPAGKIVSALKGLNGKLKADADFEGFANAIMT
                     TDTFPKYVVRKIGKATLVGVAKGSGMIEPNMATMLSYFFTDAKLSASTLKKILQSAAD
                     ATFNSLSVDTDTSTSDTVMVMANGLAGSVSEKVFAKAFSEVATELTRMLARDGEGATK
                     LFTVTVRNAANAAQAKAIGKSIINSPLVKTAIYKGDPNWGRIFMAVGKTPGVRVNPEK
                     IKIRWGGKVYTNDQLAELSKYLQSNEELRLEVSLGVGKASWTVYGCDYTEEYVAINAY
                     YTT"
     gene            complement(1943..2434)
                     /locus_tag="PLF85_10345"
     CDS             complement(1943..2434)
                     /locus_tag="PLF85_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNE20111.1"
                     /translation="MASESSFDIVSEMNWQELANAIDQAQREIGNRFDFKGTKVSVTL
                     ENKELILIADDEGKLKQLKDVVETKLIKRGIALKSVDYQKIESALGGTVRQKAIFING
                     IAQEHAKKINQLIRDSKLKVRSQVEGEKLRVFGKSRDDLQEVMKLLNGADLAIPLQYT
                     NYR"
     gene            complement(2462..3457)
                     /locus_tag="PLF85_10350"
     CDS             complement(2462..3457)
                     /locus_tag="PLF85_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE20112.1"
                     /translation="MLRYKADILSVFYMIITTALLIVNWNLPEFNVWLFLGSIMMATS
                     CFAMAHNHNHIPMWRIDFLNKLTDYWLTLFYGFPVYAWIPTHNMNHHQLNNREGDYTI
                     TWRLSEKNNFLTMITFPIVSAIYQQKPTSDFLKHYWRTNKPKFFYYISQYVVWGLWIG
                     GALYLDSRKALLYVITPMVVATQFVLIINYIQHVHTDEESQFNHSRNFTGLYNRFLLN
                     NGYHTAHHEQMGLHWSQLPAAHDKIKHLIDPSLNLKSLGWYVVKTYFLAPFIPSFRSK
                     SMRLERLALAGGGATVPVEEYHPRAVSVARVPAKRTKARKAAPKKAKSRQSKKSR"
     gene            complement(3546..4121)
                     /locus_tag="PLF85_10355"
     CDS             complement(3546..4121)
                     /locus_tag="PLF85_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE20113.1"
                     /translation="MLIRPADPQRDAQAVAGLIYDTDPYVFPFMFGRRARALPVLAQL
                     FALKANSFSHRYVTVAEADGEIAGMLIGYEPRQIDKHAEEGDFKCVLSAFEQLLMIPK
                     FWILQPFLDKSEITGRYIQNVCVAESHRGKGVGSGLIRHFCAQQPGAVWLDVEMGNKE
                     NRAIYEHMGFRVVREIPIFLPGLGSIRMVRE"
     gene            complement(4166..5368)
                     /locus_tag="PLF85_10360"
     CDS             complement(4166..5368)
                     /locus_tag="PLF85_10360"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE20114.1"
                     /translation="MLRAMSFFVLLFTALPLTADRHPVEDTVVSLPDAVSLGVGGSAK
                     LDLEIRVPKGHHAYIRHANANGRAIPISFRVPAATGFQLSVSAEPKGVRLGDEIVLRG
                     KGSYALELSELAMNEFGKTYAVPVQIRVQLCGEGEAAICYMPETIEKQLQVKLDAPEI
                     LRRAEVDTSLPWIDNRAAAIEAAQLKKQNIFALISDPTRCGACVQLETKVLPNPSVNK
                     LLREKFVLYRVPKNEYSYAPIKGRFGIPFYFVVSPDGQNLQKWVGVPGPMQFAHGIEP
                     YALASSTTPLAVPVATNAIPLNSGGRQCVIPLKQSFAFQATQKGDFKSSGNMRFVPNN
                     SAPGTYTVLTLDRTGEIENSNNARIASGTLVVERYLGGSDLVFECSPHGIAGQVTAQS
                     LAVQIELR"
     gene            5485..6120
                     /locus_tag="PLF85_10365"
     CDS             5485..6120
                     /locus_tag="PLF85_10365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE20115.1"
                     /translation="MSNEHNGWYNKVFSAAYDPFMSVVEKNALSGRRQALLSNLTGKV
                     LELGCGTGINFAFYPAEAEVLAIEPADAMRAQALEKLQKEQFAARIRVEPWLLEDERL
                     AAEYPAGSFDAAVCTLVLCTVPDPAGTLALLHRFLKPGGKLIVLEHVRSKSGFGQTVQ
                     NLMNPFWRHLAEGCQLNRDTHANIEAAGFRAATHDEFDYGLPFISGVYQAC"
     gene            complement(6143..>6536)
                     /locus_tag="PLF85_10370"
     CDS             complement(6143..>6536)
                     /locus_tag="PLF85_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE20116.1"
                     /translation="NTPILPALERGSREIVVVLLSPVGGMSMAIPKSSKKIAELILEQ
                     SLLGSYQAYMAGIHSEKQMHAELREQGILGTLQSMIRPRIVSGDTKILTVAPRKMLGV
                     ASMLNFSRAQAEELLWLGYEDAKEQLDF"
BASE COUNT         1441 a         1808 c         1797 g         1490 t
ORIGIN      
        1 gctgagcgag cggcgtttgc cggcaaatgt gaacaccgag ttgccattgc caattcgcct
       61 gacgtctacc gccgatccaa tgacctcgcc cgcctcattg agaaccgtgt attccttacc
      121 cggctcgagg gccgccgcag cactgatttc agcaatgtgc gtgaccgggt cgattcgctc
      181 gacgcgacca accgtgcgca cccctgcggc cggcaacaag gcaggcagaa ggagaatggt
      241 gatcagaatg cgggaaacca cactctattt tcggcatttt ttccggattt cccaatgcgt
      301 cagaggctcg cctcagaggc cgcgagacct ggccgggaag caataaaagt ggatgttgga
      361 ttccaaaata ggtaaatttt ctcaatgaag aatccgcagt tagtccatcg cttgagccga
      421 atacaggggc agattgaggc gctgaagcgc attgctgcat ctgaagaagc ggattgcctg
      481 aagaccattg aactcgcaaa ggctgcgtcg aatgcgatca aaaagtttgc ccaagcctac
      541 gtcgaagagc acctggagca gtgcgtcgaa gaaaagcgtg cgctcaaaga aatccagact
      601 gaactgcgca aggttgtcca gtcttctttc tcgctctgac tctacccgat cgccgctggc
      661 aatttctgcc gccggcgatc aggtcgtgta atacgcattg attgccacgt actcttctgt
      721 ataatcgcag ccgtacacgg tccacgaggc cttacccacg cccagcgaca cctcgagcct
      781 tagctcctcg ttggattgga ggtatttgga gagttcggcc agctggtcgt tcgtgtagac
      841 ctttccaccc cagcggattt tgatcttctc aggattaacc cgtacaccgg gtgtcttgcc
      901 gaccgccata aagatacggc cccagttggg gtcacccttg tagatcgcgg tctttaccag
      961 cggcgaattg ataatgctct tgccgatcgc ctttgcctgc gcggcattcg cagcattcct
     1021 gaccgtgacg gtgaaaagtt tcgtcgcgcc ttcgccgtcg cgcgcgagca tgcgagtgag
     1081 ttccgtcgcg acttcactga aggctttggc aaacaccttc tcgctaacgc tgccggcgag
     1141 cccgttggcc atgaccatca cggtgtcgga ggtagaagtg tctgtgtcga ctgacagcga
     1201 gttgaaagtg gcatcggctg ctgattgaag aatcttcttg agcgtcgagg cagacagctt
     1261 tgcatcggta aaaaagtaag acagcatcgt cgccatgtta ggctctatca tgccagatcc
     1321 cttggcaacg ccgacgaggg ttgccttacc gatcttgcgg acaacatatt tgggaaaggt
     1381 atccgtcgtc atgatggcat tggcaaaacc ctcgaaatcg gcatcggcct tgagtttgcc
     1441 attcagtccc ttgagcgccg agacaatctt gcctgccggc aaccgccgcc cgatcacccc
     1501 cgtcgacgag ggaaacacct gcgtgaccgg tattttgagt tcatcggcca cagccttgca
     1561 tacctgcaca caatcggcat aacccggttt gccggtcgcc acgttcgagt tcttcgagtt
     1621 cacgatgagc gcggtaactt tgccaccacg gatgtgcttc atgccgacct gtaccgggtt
     1681 accctttacc tggttcttgg taaaaatggc tgcagccgtg gcgggaactt cagagaccac
     1741 gacaccaaga tcgaggctgg tatcttttat gccggcattg gtgatgaagc tacgaaaacc
     1801 gcgcggagcg gctgtcagtg gtttcgattt gaaactttca gaaatactcc ggttttcgat
     1861 agccatggcc ggagagaggg gaaagtgaag tcagggggga aagcgaatta acagataacg
     1921 atcgcggcca aagccgggat cgttatctgt agttggtata ttgcagcggt atagccagat
     1981 cggctccgtt caagagcttc atgacttcct gcaggtcgtc gcgtgacttg ccgaacacgc
     2041 gcaacttttc cccttcgacc tgcgagcgca ctttcagctt cgaatcacgg atcagctggt
     2101 tgatcttctt cgcgtgttcc tgcgcaatgc cgttgatgaa gatggctttt tgccgaaccg
     2161 tgccgccgag cgccgattcg atcttctggt agtcgacaga cttgagcgcg atgccgcgct
     2221 tgatgagctt cgtctcgacg acatctttga gctgcttgag cttgccctcg tcgtcggcaa
     2281 tgagaatcag ctccttgttt tcgagggtta cagagacttt ggtacccttg aagtcaaaac
     2341 ggttaccgat ctcgcgctgc gcctggtcaa tcgcattcgc gagctcctgc cagttcattt
     2401 ctgaaacaat gtcgaatgag gattcggacg ccatggggtg tgccgccaaa gcaagaaaag
     2461 cctatcggct tttcttgctt tggcgtgatt tcgctttctt aggtgcagcc tttcttgcct
     2521 tagtcctctt cgccggcacg cgggctaccg acacggcgcg gggatggtac tcttctacag
     2581 gtaccgttgc gccgccgccg gcaagagcca ggcgctcgag gcgcatggat ttgctgcgga
     2641 aagaagggat gaaaggcgcc aggaagtacg ttttcaccac ataccagccg agcgacttca
     2701 ggttgagcga agggtcaatc aggtgcttga tcttgtcgtg tgcagcgggc agctggctcc
     2761 agtgcaggcc catttgctcg tggtgcgcag tatggtagcc gttattgagc aggaagcggt
     2821 tgtagagacc agtgaagttg cgcgagtggt tgaactgcga ttcttcgtcg gtatgaacgt
     2881 gctggatata gttgatgatc agaacgaact gtgtcgcaac gaccattggg gtgatcacgt
     2941 agagcagtgc cttgcgtgaa tcgaggtaca gagcgccacc gatccagagg ccccagacaa
     3001 catactgcga aatgtagtag aaaaacttcg gcttgttggt gcgccagtag tgcttcagga
     3061 agtcagatgt tggcttctgc tggtaaatcg cgctcacaat cgggaacgtg atcatcgtca
     3121 aaaagttgtt cttctctgac aggcgccagg tgatggtata gtcgccctcg cggttgttca
     3181 gctggtggtg gttcatgttg tgcgttggga tccacgcgta gaccgggaaa ccgtagaaca
     3241 gcgtgagcca gtagtcggtc agcttgttga ggaaatcgat gcgccacatc gggatatggt
     3301 tgtggttgtg cgccatagcg aagcagctgg tcgccatcat aatggagccc agaaacagcc
     3361 agacgttaaa ttctggcaga ttccagttca cgatgagcag cgccgtcgtg atgatcatgt
     3421 aaaatactga caaaatgtcc gctttgtatc taagcatatg atgcagtgcg caatttttag
     3481 tcaccctgtc aatcattcac ccaatggagg tgccccagga accttcgccc tgccgcagtg
     3541 attaattatt cacgcaccat acgtatactg cctaagcccg gcaggaagat cggaatctcg
     3601 cgcaccacgc gaaatcccat atgctcatag atcgctctat tctctttatt acccatctcg
     3661 acgtcgagcc agaccgcgcc tggctgctgt gcacagaagt ggcggattaa ccccgaacct
     3721 acgcctttgc cccggtgcga ttcggcgacg cagacgttct ggatatagcg accggtgatt
     3781 tcactcttgt cgagaaacgg ttgcagtatc cagaatttgg gaatcatcag cagctgctcg
     3841 aaagcggaga gcacgcactt aaaatccccc tcttctgcat gcttgtcaat ctgccgtggc
     3901 tcgtagccga tcagcatgcc ggcgatctcg ccgtctgctt cggctacggt cacgtaccgg
     3961 tgcgagaacg aatttgcttt gagcgcaaat aattgggcca gcacaggcag cgccctcgca
     4021 cgccggccga acatgaacgg gaaaacgtac ggatcggtgt cgtagatcag gcccgcgact
     4081 gcctgcgcat cacgttgcgg gtctgccggt cgaatgagca tttgcccggc tgcagtttgc
     4141 cggtgcgacg caaagtaaga acaggttaac gaagttcaat ctgtaccgcg agcgactgcg
     4201 cagtcacctg acccgcaatg ccatgcggcg aacactcgaa caccagatcg ctgccgccga
     4261 ggtagcgttc gacgacgaga gtaccgctgg caatacgtgc gttgttcgag ttttcaattt
     4321 cgccggtacg atcgagcgtc agcaccgtgt acgtaccggg tgccgaattg ttgggcacga
     4381 aacgcatatt gccggaactt ttaaaatctc ccttttgcgt tgcctgaaag gcaaaactct
     4441 gcttcagagg aatcacgcac tgcctgccgc ctgaattgag cggaatcgca tttgtagcga
     4501 cgggaactgc cagcggagtt gtgctgctgg caagggcata gggttcgata ccatgtgcaa
     4561 actgcatcgg ccccggcacg ccaacccact tctgcagatt ctgaccgtcg ggcgacacca
     4621 cgaaatagaa cggaataccg aaacgtccct tgatgggagc atagctgtac tcatttttgg
     4681 gtacgcggta gagcacaaat ttttcacgca acagcttatt aaccgaagga tttggcagga
     4741 cttttgtttc gagctgcaca caggcgccgc atcgcgtggg atcagatatc agggcaaaaa
     4801 tgttctgttt cttcagttgc gcagcttcaa tagcggccgc gcggttgtct atccatggca
     4861 ggctggtgtc gacttctgca cggcgcagta tttccggtgc atcgagttta acctgcagct
     4921 gcttttcaat agtctcgggc atgtagcaga tcgcggcttc accttcgccg cacagctgta
     4981 cacgaatctg cacgggcacc gcgtaggtct tgccaaattc attcatcgcg agctctgaca
     5041 gttcgagcgc gtaactaccc ttgccccgaa gaacgatctc atcgccgagg cgcacaccct
     5101 ttggctcagc tgaaaccgag agctgaaaac ccgtcgctgc aggcacgcga aaactgatcg
     5161 gaattgcgcg gccgtttgcg ttcgcatggc gaatgtaggc gtgatgcccc ttcggtacgc
     5221 ggatttccaa atcgagcttc gcgctgccac cgacaccaag cgagacggca tcgggtagtg
     5281 atacgacagt atcttcaacc gggtgccgat cagcagttaa cggcagtgcg gtaaaaagca
     5341 gcacgaagaa actcatggct cgtaacatgg ggtaatcccc ctcaccatgc cggtcgcgta
     5401 aagaccaaat gcccctcggc agacactaga ctgtgacaca accccgcttg tcgcccgcgc
     5461 tgccttcata agctcgccgc agcgatgagt aatgagcaca acggctggta caacaaggtc
     5521 ttctctgcag cctacgaccc cttcatgagc gtagtcgaaa agaatgcgct gagcggacgc
     5581 cgtcaggcat tgctcagcaa cctgacaggc aaagtactcg aactcggttg tggcactggt
     5641 atcaattttg cattctaccc cgcagaggct gaagtgctgg cgattgaacc tgctgacgcg
     5701 atgcgcgcgc aggccctgga gaagttgcag aaagaacagt tcgccgcgcg cattcgtgtt
     5761 gaaccgtggc tgctcgaaga cgaacgcctt gcagccgaat atcctgccgg cagttttgac
     5821 gcggcggtct gtacgctcgt gctctgcacg gttcccgacc cggccggcac actcgcgctg
     5881 ctgcaccgct ttctcaagcc cggtggcaaa ctcattgtgc tggagcacgt gcgttcaaaa
     5941 agcgggttcg gccagacagt gcagaacctg atgaacccgt tctggcgtca cctcgccgaa
     6001 ggctgccagc tcaaccgcga tacacacgcg aatatcgagg cagcaggctt tcgcgctgcg
     6061 acgcacgatg agtttgatta tggtctgccg tttatctctg gggtttacca ggcctgctaa
     6121 ccggtttctt ttcaggagcc tgttagaaat ccagctgctc tttcgcatct tcataaccga
     6181 gccacaacag ttcttctgcc tgcgctctcg aaaaattcag catcgaggca acgcccagca
     6241 tctttcgggg cgccacggtc agaattttcg tatcgcctga aacgatgcgt ggccgaatca
     6301 tgctctgtaa tgtgcccaga ataccctgct cgcgcagctc agcgtgcatt tgtttttcag
     6361 aatgaattcc cgccatataa gcctggtacg acccgagaag cgactgttca agaatcagtt
     6421 cagcaatttt cttgctgctt tttggtatcg ccatgctcat gccgcctacc ggcgaaagca
     6481 gcacaacaac gatctctcgc gacccgcgct ccaacgccgg taagataggc gtgttc
//
LOCUS       DAOQJF010000184         6510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6634_length_6510_cov_6.809140, whole genome shotgun sequence.
ACCESSION   DAOQJF010000184 DAOQJF010000000
VERSION     DAOQJF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6634_length_6510_cov_6.809140"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLF85_10375"
     CDS             complement(<1..739)
                     /locus_tag="PLF85_10375"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNE20117.1"
                     /translation="MRLVILLFALFIVACSSTGKRDKTAEAPTKAQTSESRSMLTDDE
                     ESEGEMAVSDPWEGFNRKMHAFNDFFLHYAINPLARGWAFITPTFLREGLNNFFYWAY
                     TPGRLVNNLAQAKFKGAAKETAGFMINGTAGMLGFYDAARDIFSLERTNEDTDQTLGK
                     WGLPEGPYVVWPFIGPHTVRGTFGFAGDLFLQPQTVVVPVYIKPETLWVEGAIIAGVY
                     SFRAINRTSLDVDAYDNLIKDAIDPYGF"
     gene            1710..3806
                     /locus_tag="PLF85_10380"
     CDS             1710..3806
                     /locus_tag="PLF85_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE20118.1"
                     /translation="MQNAFTNPLVALHEVTLSLLTQDSSDKLLNALLDRALEFTGADS
                     GTISILDDSRKFLEIKAVRGFEQDLPRTVKLKIGEGVTGRCIVTGKLRNVGDTSQDKY
                     YIAVRQDIRSELAVPLKVGNKSFGVISVDSKKLDAFNAEHEEYLEMLAEYAAQIFTTT
                     TSVESLSHRTHLLEVLLQVSGVLGKYADVKLFFEEVIRILSEKLGVRRAAIYLYDSVT
                     NELSVAASLNYSADEVAKGRYAPGEGVTGKAFKQRKAIAVADISHDNQFLNKTGHQRD
                     ETVSFFAAPFFEGGEIVPAGQSNAGGVFNFEIKFQSQSKHEDYQFLAQILSSLFGQAM
                     QIEKLVQQSSREVKEENIILRRQLKSTFEFESIVGAHPRMADLFARMKMAADSASAVL
                     ITGESGTGKELIASALHQNSLRAANALVKINCAAIPADLLESELFGYARGAFTGAVDD
                     YKGKVLSAHKGTLFLDEIGELDLKLQAKLLRVLQEKEFSPLGSNKVIKVDVRIIAATN
                     ANLESAVKEKAFREDLFYRLNVVRLSIPPLRERLSDLPLLIQHLLDKICERNGKKISG
                     VTDAAFRKLQTYSFPGNIRELENILERAVVLNAKTEVDSEDIQLGDGAVLAGNEPVVA
                     ATAQAFDLRSYLKDEIARTPSGEIFEGVIGGVEKELLRILLNQNQFNKRKTAEALGLN
                     RVTLDKKIQQYGLFEI"
     gene            3919..4905
                     /locus_tag="PLF85_10385"
     CDS             3919..4905
                     /locus_tag="PLF85_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE20119.1"
                     /translation="MGPKIVVFEPDGIYRTIIENILLKNNYRPYFVDNSAECKVVVDE
                     IRPDIIIIDIRAAKPHDFRILADLKVLSKAPVLLTTEENVDDYMTALGSKNVTIVLPK
                     PIKGAELSKVLANLLTVDPRVWFGIHNYIPDLRKLRKMKLKRSTQVRTCIKTILNEMH
                     AWGFNFNHETEMDLVWQEILINAVYHAHGYTNEKKERVAIELPQPYEVEVTYGASEHA
                     FGIAVRDSMGTLTPSIIIEALRQTVEQQKMIEKAAETGEDISHLVLDRGRGLDLIRQL
                     SGEYYFIIDPGVSTEVIIIYDRYYEKDDAVGSLKIFDLSYHLRDTPVIAEPG"
     gene            4874..5197
                     /locus_tag="PLF85_10390"
     CDS             4874..5197
                     /locus_tag="PLF85_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20120.1"
                     /translation="MTPLLSQNQAKAGSAGSREPVTITIQGLRDENAEKSCLKAINRN
                     RNNPLSFAEKGDGDYQIFIEKKRPGYQVRVEKQGTALRTGRALHDFEVCIAAAAMARE
                     AIADE"
     gene            5354..6325
                     /locus_tag="PLF85_10395"
     CDS             5354..6325
                     /locus_tag="PLF85_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20121.1"
                     /translation="MEIPRGCSAELRGNRPKIYIANFYVTYGKPDMGKAIGDYIAEKF
                     ETDGRLDVISRSIINEEMKPLLREKKLKAERYLEQTLAYAAQREADCVIFGRIARDKK
                     GKRISFLVRMAAVESGQNLRRVDTEVEREEAMGFLEKVGESFVTYFTTVTVPVAPTLE
                     VALPSSKEKRFHVAAASIGLMPLGFVKEGFPYAVGGNGEFAYHGLAKNLFVGVNAEYL
                     QYLRKDNRFESLYGIGSFGFIGYRFWGGISWHMQAVLYGGYQFGKLNGSVESTTYGYG
                     VAMGGLRTVFDVTPRFGILVEGRYVFAMAGSTQIASGAATFGGLWRF"
     gene            6325..>6510
                     /locus_tag="PLF85_10400"
     CDS             6325..>6510
                     /locus_tag="PLF85_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20122.1"
                     /translation="MKRLSFALITVVAVFTGCKSYEDSFNRNLNSTGLYVANSASNTI
                     SMFAADPKSGILNDLGTV"
BASE COUNT         1607 a         1702 c         1685 g         1516 t
ORIGIN      
        1 tgaacccata cgggtcaatc gcatctttga tgagattgtc atatgcgtcg acatccagcg
       61 atgtacggtt gatcgcgcgg aacgaataaa ctcccgctat gatagcaccc tctacccaga
      121 gcgtttcagg cttgatatag accggtacaa caactgtctg cggctgcagg aaaagatcgc
      181 cggcgaaacc gaacgtgccg cgtacggtgt gcggtccaat gaatggccag acaacgtaag
      241 gaccttccgg cagcccccat ttgccgagcg tctggtcggt atcctcgttg gtgcgctcca
      301 gggaaaagat atctctcgcc gcatcataaa acccgagcat acccgcggtg ccgttaatca
      361 tgaaaccggc tgtttctttt gctgcaccct tgaacttggc ctgcgccagg ttgttcacca
      421 gccgcccggg tgtgtacgcc cagtagaaaa aattgttcag cccctcgcgc agaaacgtcg
      481 gggtgataaa cgcccagccg cgcgcgagcg gattaattgc atagtgcaga aaaaaatcgt
      541 tgaatgcatg catcttgcga ttgaagcctt cccaagggtc actgactgcc atttcacctt
      601 cactctcttc gtcgtcagtg agcattgaac gcgactcgct tgtttgcgct tttgtgggag
      661 cttctgcggt tttgtctctt ttgcctgtgc ttgaacaggc tacaatgaat agcgcaaaga
      721 gcaggataac cagacgcata atgaaacgca cgattctgta aacccgttta tgaaccggaa
      781 gcgtaaattc aattttggta ctgccaggtt agcccaagat tgtgccccag caggattttt
      841 gcccatctat gggggcatga agatactttt ccgttaaaaa gaacaggagc agtgtgcagt
      901 tttttatggt tcggcctgtg tttttgtctg aaagcccggg ttaaatagtt catgttttcg
      961 caggacaagg gcttcctgtc ggtaaatgag cccggagtta tcattttttt cggtggagca
     1021 ttggtaggta ataaaaattt ccgcctgagg cttgcaccgg cgctcaaaac gcatcttcca
     1081 gtaatgcgta ttacgacccg accgcaaaac tcccggtatt tcactgcgca ggtactcctc
     1141 caacagcctg ggcaggtagt gccgaatcgc gaaatccagc atgcgcgaaa tagattcacc
     1201 ggagtggact gccctctccc acaaagtgga atacagtagc ttgtctatgt accatgggat
     1261 gcgaacatac ttttcgccct gaccgtggtt gtaccgccgc gccgttgcgg attttacacc
     1321 gcgaccgcgc cagtgccgca ggtacatttt ggccaatcga cgaagcagct ccgactcgga
     1381 ccacaatatg ccattgcgct tcaggatcaa caacccagct ctaaagcggc gccttgaggt
     1441 cggccggaga gtcaccgatg tatggtggta ttgcaggctg gtgataggtt gcattctata
     1501 ttttaaatac cggagaaacg gcatttcttc tcactcagct tcgatttttt ttcgtgatag
     1561 cccaataatt taacaagagc gtgtgcacaa gaatatgctt tctatttgat tttatcccat
     1621 tttcgtatgc gtttccgaca atatgcactc gtcactcaca aaatgcataa aatctttgca
     1681 ccttgtcgca aagttcgcct ctggtggctg tgcagaacgc tttcaccaac cccctcgtcg
     1741 cactgcacga ggtaacactc tccctactaa ctcaggattc cagcgacaaa ctcttaaatg
     1801 ctctgctcga ccgtgccctg gaattcaccg gtgcggattc gggaaccatt tctattctgg
     1861 atgactcgcg aaaatttctg gaaatcaagg ccgtgcgcgg ctttgagcag gaccttcccc
     1921 gcacagtaaa actcaaaatt ggcgaaggtg tcaccgggcg gtgcatcgtg acgggaaaac
     1981 tgcgtaacgt gggagacact tcgcaggata agtattacat cgctgtgagg caggatatcc
     2041 gctctgagct ggccgtacct ctcaaggtag gtaataaaag ttttggcgta atttccgtcg
     2101 actcgaagaa actcgatgca ttcaacgccg aacacgagga atacctcgaa atgctcgctg
     2161 agtatgcggc gcaaatcttt actacgacta cctcggtaga atcccttagc caccgcaccc
     2221 acctgctcga agtactgctt caggtcagcg gagtgctcgg taaatatgcc gatgtgaaac
     2281 tgttttttga agaagttatt cgcattctct cggagaaact gggtgtgcgt cgggctgcca
     2341 tctatttgta tgattccgta acgaatgaac tctcggttgc cgcttcactc aactacagcg
     2401 cggacgaagt tgccaaaggc cgctacgcac ccggtgaagg cgttaccggt aaggcattca
     2461 agcagcgcaa agcaattgcc gtagctgaca tttcgcacga taaccagttt cttaataaga
     2521 ccggccacca gcgcgatgag acagtgagct tctttgcagc accatttttt gagggaggcg
     2581 agatcgtgcc tgccggtcaa tcaaatgcag gcggagtttt caatttcgag atcaagttcc
     2641 agtcgcagag caagcacgaa gattaccagt tcctggcgca gattctgtct tctcttttcg
     2701 gccaggcgat gcagatcgaa aagctggtac aacagagctc gcgcgaagtt aaagaagaga
     2761 acatcatact gcggcgccag ttgaagagca cctttgaatt tgaaagtata gtgggagctc
     2821 acccacgtat ggcagattta tttgcgcgca tgaaaatggc cgccgactct gccagcgcag
     2881 ttctgatcac gggtgaatcg ggtaccggta aagaacttat cgcgagcgca ctccaccaga
     2941 acagcctgcg tgctgcgaac gcactggtaa aaatcaattg tgcagctata ccggcagacc
     3001 tgctcgagag tgaactcttt ggctatgcaa ggggagcttt cacaggagcg gtcgatgact
     3061 acaagggcaa agtactctcc gcgcacaagg gtacgctgtt cctcgacgag attggcgagc
     3121 tcgatctgaa gcttcaggcc aaactgctgc gggtgctgca ggaaaaggaa ttttcgccct
     3181 tggggagcaa caaggtcatc aaggtcgatg tccgcattat tgcagcgacc aacgccaatc
     3241 tcgagagcgc cgtcaaagag aaggcattcc gcgaagatct cttctaccgg ctaaacgtgg
     3301 tcaggctttc tatacctcca ctgcgtgaac gcctctccga cctaccgttg ctcatccagc
     3361 acctgctcga caaaatctgc gagcgtaatg gtaagaagat cagcggtgtt accgatgcag
     3421 ctttcagaaa actgcagaca tattcgtttc ccggcaacat cagggaactc gagaatatcc
     3481 ttgagcgtgc tgtggtgctc aacgccaaaa ccgaggtcga ttcagaagac atacagctgg
     3541 gtgacggcgc tgtattggct ggaaacgagc cggtggtagc agccaccgca caggcatttg
     3601 accttcgcag ttacctcaaa gacgagattg cacgtactcc atcgggcgaa atttttgagg
     3661 gtgtcatcgg cggggtcgag aaggagctcc tgcgaattct gttaaaccag aaccagttca
     3721 acaagcgtaa gacagccgag gcgttggggc tgaaccgcgt cacactcgac aagaagatac
     3781 aacagtatgg cctgttcgag atttaacttc cctgtattat gtcgcataac aacgctatgg
     3841 accttcctaa tcggtaacca cgggcgacaa aaaagctgac tcccacgaaa tttttcggtc
     3901 gatactgaaa aggtagcaat gggcccaaag atcgtcgtat ttgaaccgga cggtatctac
     3961 cggacgatta ttgagaatat ccttctcaag aataactacc gcccgtactt tgtcgataac
     4021 tccgccgaat gcaaggtggt tgtcgacgaa attcgccccg acatcatcat cattgacatt
     4081 cgtgccgcca agccgcacga ctttcgtata cttgccgatc tcaaggtact gagcaaggcg
     4141 ccggttctgc tcaccaccga agagaatgtc gacgattaca tgaccgccct gggcagcaag
     4201 aatgtgacga ttgttctgcc gaaacccatc aagggtgccg agctcagcaa agtgctcgcg
     4261 aacctgctga cagtcgaccc gcgggtctgg ttcggcatcc acaattacat tcccgacctg
     4321 cgcaaactgc gcaagatgaa actcaagcgc tcgacacagg tgcgcacctg catcaagacc
     4381 attctgaacg aaatgcacgc ctggggtttc aacttcaatc atgaaaccga aatggatctt
     4441 gtctggcagg aaattctcat caacgcggtc taccatgcac acggctatac taacgagaaa
     4501 aaagaacgcg tcgccattga gctcccccag ccttacgagg tagaagtaac ctacggcgca
     4561 agcgagcacg cgtttggcat cgcggtgcgc gacagcatgg gcactcttac cccttcaatt
     4621 atcatcgagg cgcttcggca gaccgttgag cagcagaaga tgattgaaaa ggctgccgaa
     4681 acgggtgaag atatttctca cctggtgctt gatcgtggcc gggggctcga cctgatacgc
     4741 cagctttccg gggagtatta tttcatcatt gaccccggtg tttcgaccga agttattatt
     4801 atctatgacc ggtattacga gaaagacgat gccgtcggat ctctcaagat ttttgatctt
     4861 tcttaccatc tgcgtgacac ccctgttatc gcagaaccag gctaaggccg gttcagcggg
     4921 cagccgcgaa cccgtaacca ttaccataca gggccttcgt gatgagaacg cagagaagag
     4981 ctgtctcaaa gccatcaatc gcaatcgcaa caacccgctg agcttcgccg aaaagggcga
     5041 cggcgattac cagatattca tcgaaaagaa gcgcccgggc taccaggtcc gggtggagaa
     5101 gcagggcact gcactgcgta caggccgcgc gctgcacgac tttgaagtat gcattgcggc
     5161 agctgccatg gcgcgcgaag ctatcgctga cgaatagatt acctgaagaa aattgttggc
     5221 aactccacca ttacgtacgt ctaactccaa aggaaacatg cgtcgcctgt ttgttatcgc
     5281 ggttttttgc ctgggtattt gccagatttg gtcacagggg aatgcatccg ccccggctgt
     5341 caatgtgtcc cgaatggaaa taccgcgcgg ctgttcggcc gaacttaggg gtaaccgccc
     5401 gaagatctat attgcaaatt tctatgtgac atacggcaaa cccgatatgg gtaaggcgat
     5461 cggcgactat attgcagaga agttcgagac cgatgggcgc ctggacgtca tttcgcggag
     5521 cataatcaac gaagaaatga agccactgct gcgtgaaaag aaactcaagg cagagcgcta
     5581 cctcgaacag acgctggcat acgcggcgca gcgcgaagca gattgtgtca tctttggccg
     5641 cattgcgcgc gacaagaaag gcaagcgcat ttcattcctc gtgcgtatgg ctgcggttga
     5701 atcaggtcaa aacctgcgca gagtcgatac cgaggtagaa cgcgaagaag cgatggggtt
     5761 tctggaaaag gtcggggagt cgtttgttac ttacttcacg accgtcacag tgccggtggc
     5821 cccaacgctt gaggtcgcgc taccgtcatc gaaagagaaa cggtttcatg tcgccgccgc
     5881 atccataggc ctgatgccgc tcggcttcgt gaaagaaggc tttccttacg ccgtgggtgg
     5941 taacggtgaa ttcgcctatc acggccttgc gaagaatctt tttgtgggag taaatgcaga
     6001 gtacctgcaa tacctccgta aagacaaccg cttcgaaagt ttatacggca ttggttcgtt
     6061 tggctttatc ggctaccgtt tctggggcgg tatttcatgg cacatgcagg ccgtgctcta
     6121 cggaggctac caattcggta aactcaacgg ctcggttgaa tcgaccacct atggctacgg
     6181 tgtcgccatg ggtggcctgc ggacagtgtt tgatgtgacc ccgcggttcg gtattctcgt
     6241 tgaaggccgc tatgtctttg ccatggctgg ctcaacacaa attgcatcgg gcgctgccac
     6301 cttcggcgga ctgtggagat tctgatgaaa cgtctcagct tcgctcttat cactgtcgtc
     6361 gcggttttca ctggctgcaa gtcttatgaa gacagcttta accgcaatct caatagcacc
     6421 ggactctatg tggcgaattc cgcatcgaat acgatttcca tgttcgctgc cgaccccaaa
     6481 tctggcatat tgaacgacct gggcacggtt
//
LOCUS       DAOQJF010000185         6499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6650_length_6499_cov_5.019863, whole genome shotgun sequence.
ACCESSION   DAOQJF010000185 DAOQJF010000000
VERSION     DAOQJF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6650_length_6499_cov_5.019863"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..74
                     /locus_tag="PLF85_10405"
     tRNA            1..74
                     /locus_tag="PLF85_10405"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Met,seq:cat)
     gene            144..1502
                     /locus_tag="PLF85_10410"
     CDS             144..1502
                     /locus_tag="PLF85_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE20123.1"
                     /translation="MSVSRKEKLERLRLALSISYRYSRGKSRYFLSMNAFLAFVGVFV
                     GTSLLVVVMSIFGGFQAQLKKSIFQFDPHVMLTVDRITGGEKIAGWQKWKDRLAADLK
                     DDVDSVEGSIQSPAIFRRRKFLDHVFVRGVDFPINEKSKMYDVPAYFPEIVEPALSRS
                     IPQGDYAIIGREMALNLGLSVGDTIELIVPRGQFSLQTGVTPSIKTFKVAALFKTGHY
                     NYDTKVVVLSLQTAQNLYGLPDAVQQLVLRVKDTDHLTKIRRKVARAWPYTIRTIEDE
                     QRNFFAALRLEKTIMVVIVFLFIIAAMVGIVIATFNIVRAHRRDIGIMKSLGFSRRHI
                     LIIFAVTGMAMGVLGSILGIFAGVGLANNLENILHAIEGVINPIGKFYAENISHNIWT
                     ELRLIPRDVYYFDHLPIDIDWGLLRVLGYVSVILSILASVIPARKAARLEPIDIIRKA
                     EI"
     gene            1502..2209
                     /locus_tag="PLF85_10415"
     CDS             1502..2209
                     /locus_tag="PLF85_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE20124.1"
                     /translation="MESQYNPDRPILEVSDLHKTYRDGEIMAEVLKGITFSVAPGEFV
                     AIQGPSGAGKSTLLNLLGALDKPDSGKIVIDGLELTSAVRADRVDLFRRDKIGFIFQN
                     HYLMQEFTVVENVMLPLRVQGKSYAEAKEIAARVLTRVGLGHRMEHYPSQISGGESQR
                     VAVARAVVKSPPLILADEPTGNLDSDNRARFIEVLNTLQAEDHLTVIVVTHESELAMA
                     ASRRLLMRDGQLISENS"
     gene            complement(2250..3848)
                     /gene="lnt"
                     /locus_tag="PLF85_10420"
     CDS             complement(2250..3848)
                     /gene="lnt"
                     /locus_tag="PLF85_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNE20125.1"
                     /translation="MRRILFLSAVSAVLTSLSFVPYNLFPLGFISLLPVFYVFETTPL
                     TRRQVTWFWLVWIMILNLVGYHWIMHTIAVYGMMPQAVAFPLFLLYSLGTGTKMLFFF
                     FFLRLLAQQRDFFSGTAFRTATVVAAFGLFELIGWQLFPWYGSNLVSGDLWFAQGADL
                     FGTRGLSLIWILIQYLTWNTVRDFISLDRQWRPLLLQSRSLQLLGALLFVTHLYGFIQ
                     VQRFSALEEAAPKISIGVPQGNVPLITSYHERPYIISRMIAQTQRLISDAAARGEKTD
                     LVVWPESSIPAMEYERSAELRSAITSLQKETQTPLIINDIYHDPATRKDYSNMWQLDH
                     EGNPMASYQKNFLLPFGEFMPLGNTFPALKNLFPAVSDFSHGQRFNLFSLATKHGTVK
                     AMPLVCYEIILPDYARGFDARTDHSAQFIINITNDAWFGDSYESLQHMTLGTMRAIEL
                     RLPILRSTNSGISSYTSTTGEVFGQTKMFERVNQVYRVPVMERSVTLFALAGNWPFWI
                     YLFLTAGLVAYRFSRIQKDSRTGS"
     gene            complement(3848..4339)
                     /gene="coaD"
                     /locus_tag="PLF85_10425"
     CDS             complement(3848..4339)
                     /gene="coaD"
                     /locus_tag="PLF85_10425"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNE20126.1"
                     /translation="MRSAVYPGSFDPFTNGHLDIVQRAIRIVDKLTIAVLKNTSKKGM
                     FSPEERVDIIREVVRDYPQVEVETFEGLLVDFCRERKTRIVIRGLRAVSDFDYEHAIF
                     LMNRKLLPDLETMFLMAGSENSFISSTIVKEVAHFGGNIADQVPAIVFERMQKQKLQN
                     RNE"
     gene            4427..>6499
                     /locus_tag="PLF85_10430"
     CDS             4427..>6499
                     /locus_tag="PLF85_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE20127.1"
                     /translation="MTILSPQGRKVHEIFHPAVSNYFERTFGAPTPVQLKAWQVLHGE
                     NSRQHALISAPTGEGKTLAAFLAIIDRLVRQAAAGEDIGNTRILYISPLKALANDIEK
                     NLQQPLAGIRAELARMGIEIPEIQAMVRTGDSSQSERARLVRLRPQIVVTTPESFYLL
                     LTSESGRRLLSTIETVIIDEIHALVAERRGAHLALSLARLEALRRDHDAHCRPLQRIG
                     VSATVRPLERVAQYLAGNDPVRFCTDERRRPFDIRLLLPDEGLSAVMSGNHWNQIYLK
                     LTEEIRNRRTTLVFVNNRRLCERMARSLSELIGAEFVGAHHGSLSHAERRRGEERLKA
                     GSLKVMVATQSLELGLDIGSIDLVVQVSSPRSIHAFIQRAGRSEHHKQGTSRIILVPL
                     TRDDLRECVALVSALRRGELEEVKLCAPALDVLAQQVVAEVSARDYAVDELFALFTGS
                     FVYHDLQRSKFLQILQMLNDGFSLRFGRRSRYLFFDQTAGTLHARPGARLIAALNGGA
                     IPENFEYEVVDETDQVYVGSVHEDFAIESMRGDVFQLGNRLWQIVQVSGNRVSVIAAP
                     DKHPTIPFWIVDVPGRSDTLSAAVARLNEDFLAAESADHFVTAIARETGLEKRPLVLL
                     ADYLLQAKNSLGSLPTQQQVVLERFFDEVRNCHIVLHSPFGAQVNRAWGFALRKRFCR
                     KFNFELQAA"
BASE COUNT         1428 a         1731 c         1861 g         1479 t
ORIGIN      
        1 ggcgacgtag ctcagctggt tagagcgatg gaatcataat ccacaggtcc ggggttcgag
       61 tccctgcgtc gctacatcta actctcccga atgcttttct ctcgccacgc tgttgccgtt
      121 atcaaccctg ctgacgagta gtcatgtccg tttccagaaa agaaaaactt gaacgcctgc
      181 gccttgcgct ttctatcagc taccgctatt cgcgcggtaa aagccgctac tttctcagca
      241 tgaatgcctt tttggccttc gtgggagttt ttgtcggtac gagccttttg gtcgtggtga
      301 tgtccatttt cggcggtttt caggcccagc tcaaaaagag tatctttcag tttgacccac
      361 acgtgatgct gacagtcgac cgcattaccg gcggtgaaaa aattgccggc tggcagaaat
      421 ggaaagaccg gctcgctgca gaccttaaag acgatgtcga ttctgtcgaa ggcagcatac
      481 aatcaccggc gatttttcgc cgccgcaagt ttctcgacca cgtctttgtg cgtggcgtgg
      541 attttccgat caacgaaaaa agtaaaatgt acgatgtacc tgcttatttt ccggagattg
      601 tcgaaccggc tctgtcgcgc agcattccgc agggcgacta tgcaatcatc gggcgcgaaa
      661 tggccctcaa cctgggcctc agtgtcggcg acaccataga gctcatcgtg ccccgcgggc
      721 agtttagcct gcagactggg gtaactccct ctatcaagac gttcaaggtt gcggcgctct
      781 ttaagaccgg gcattacaac tatgacacga aggtggttgt gctctcgtta cagacagcgc
      841 agaaccttta cggtctgccc gatgcggtgc agcagctggt gttgcgcgtg aaagacaccg
      901 accacctgac caaaattcgc cgcaaggtgg cacgtgcctg gccttatacg atacgcacga
      961 ttgaagatga gcagcgcaac ttcttcgctg cgctgcgtct cgaaaagacc atcatggtgg
     1021 ttattgtttt tctctttatc atcgcggcca tggttggtat cgtgattgcc acctttaata
     1081 tcgtgcgcgc gcaccgacgc gacatcggca tcatgaaatc gctcgggttc tcgcgccgcc
     1141 atattctcat tatctttgcc gtaacgggaa tggcaatggg cgtattgggg tcgatactcg
     1201 gcatatttgc cggtgtcggg cttgcgaaca acctggaaaa tattctgcat gcgattgaag
     1261 gtgtcatcaa cccgatcggc aagttttacg ccgagaatat cagtcataat atctggaccg
     1321 aactgcgcct gataccgcgc gatgtgtact actttgacca tttgcccatt gatatagact
     1381 ggggtttgtt gcgcgtgctt ggctacgttt cagtgatctt gtcgatactg gcgtccgtca
     1441 tacccgcccg caaggctgcg aggctcgaac cgatcgacat tatccgtaag gcggagatct
     1501 gatggaatcg cagtacaatc ctgaccggcc gattcttgaa gtcagcgacc tgcacaagac
     1561 gtaccgagac ggcgagatta tggccgaggt gctgaagggt attaccttca gcgtggcacc
     1621 cggtgaattt gttgcgatcc aggggccgtc gggtgcaggc aagagcacct tgctcaacct
     1681 gctgggtgcg ctcgacaaac cagattcggg aaagattgtg atcgacggtc ttgaactgac
     1741 atcggccgtg cgggctgacc gcgttgatct ttttcgccgc gacaagatcg gttttatttt
     1801 ccagaaccat tacctgatgc aggaattcac ggtcgttgaa aacgtgatgc ttccgttgcg
     1861 tgtgcagggc aaaagttacg ccgaggcaaa agagatcgcg gcgcgggtct taacccgtgt
     1921 tggcctcggg catcgtatgg agcactatcc ctcgcagatt tcgggtggtg aaagtcagcg
     1981 tgttgccgtg gcgcgcgcgg tggtgaaaag ccccccgttg atactggccg atgaacccac
     2041 aggcaatctc gattcagaca accgcgcgcg gttcatagag gtactgaaca cgctgcaggc
     2101 agaagaccac ctgaccgtca tcgtcgtaac gcatgagtca gaactcgcga tggctgcatc
     2161 gcgccggctg ttgatgcgcg acggccagtt gatttcagag aattcttaaa gtactgcgcg
     2221 acttttccta acaagctcct gaaaaggagt caggagccgg tgcgggagtc tttctggatc
     2281 ctgctgaacc ggtaggcgac caggccggcc gtgaggaaca ggtagatcca gaacggccag
     2341 ttgccggcga gcgcaaacaa cgtcacactg cgctccataa cgggtacgcg atagacctgg
     2401 tttacgcgtt cgaacatctt tgtttgccca aatacttcgc cggtggttga agtatacgaa
     2461 ctgatacccg aattggttga acgcagtatc ggcaggcgca gctcaatcgc gcgcatcgta
     2521 ccgagcgtca tgtgctgcag gctttcgtac gaatcgccga accaggcatc gttggtgatg
     2581 ttgatgataa actgcgctga gtgatcggtg cgtgcatcga agccacgcgc atagtcaggc
     2641 agtatgatct cgtagcagac gagcggcatg gcttttacgg tgccatgttt ggtagccagg
     2701 ctaaacaggt tgaatcgctg gccgtgcgaa aaatctgata ccgccggaaa aagatttttg
     2761 agagcaggaa acgtattgcc gagcggcatg aactccccga acggtagtaa aaagtttttc
     2821 tggtagctcg ccattgggtt accctcgtgg tcgagctgcc acatgttcga gtaatctttg
     2881 cgcgtagccg ggtcgtggta gatgtcgttg ataatcagtg gcgtctgcgt ttctttttgc
     2941 agagaagtaa tagcgctgcg caattcagcc gagcgttcgt attccattgc cggtattgag
     3001 ctttcaggcc agacgaccag atcggtcttt tcaccgcgcg ccgcggcgtc actgatcagc
     3061 ctttgcgttt gcgcaatcat gcgtgagatg atgtatggcc gttcgtggta gctggtaatc
     3121 agcggtacat tgccctgtgg cacgccgatg ctgattttgg gcgccgcttc ttcaagcgcc
     3181 gagaagcgct gtacctggat aaacccatac aggtgtgtca caaagagcag ggcacccagg
     3241 agctgcagac ttcggctctg cagcaaaagc ggccgccatt ggcggtcgag actgataaag
     3301 tcgcgcacgg tattccaggt gaggtactgg atcagaatcc agatgagcga gaggccacgg
     3361 gtgccaaaga gatcggcacc ctgcgcgaac cacaggtcgc cgctaacaag gttgctgccg
     3421 taccagggaa aaagctgcca gccaatcagt tcaaacaggc cgaacgcagc tacaaccgtt
     3481 gctgtcctga aagcagtgcc ggaaaaaaaa tcccgctgct gtgccagcag gcgcaggaaa
     3541 aagaaaaaga acagcatctt ggtgccggtg cccagtgagt agagcagaaa cagcggaaag
     3601 gctaccgcct gcggcatcat gccgtaaacg gcgatcgtgt gcatgatcca atggtagcct
     3661 accaggttga gaatcatgat ccacaccagc cagaaccagg tcacctggcg gcgggtaagg
     3721 ggagttgtct cgaatacgta aaaaaccggc agcaacgaga taaatcccaa tggaaaaagg
     3781 ttataaggga caaacgaaag cgaggtcagt acggctgaga cagccgaaag aaacaggatg
     3841 cgacgcatta ctcgttgcgg ttctgcagtt tctgcttctg catacgttca aagacgattg
     3901 ccggtacctg gtcggcgata ttaccgccaa agtgcgctac ctctttgacg atcgtactcg
     3961 agataaacga gttctcagag cccgccatca ggaacatcgt ctccaggtcg ggcaggagct
     4021 ttcggttcat aaggaaaatt gcgtgttcgt agtcaaagtc agaaactgcg cgcaacccgc
     4081 gaattacaat gcgcgtcttg cgctcgcggc aaaaatcgac cagcagccct tcgaatgtct
     4141 ctacctctac ctgtgggtag tcgcggacca cttcgcggat aatgtcgacg cgttcttcgg
     4201 ggctaaacat cccttttttc gaggtattct tgagaaccgc gatggtcagc ttgtccacaa
     4261 tacgtatggc cctttgtaca atatccagat ggccgttggt aaatgggtcg aagctgcccg
     4321 ggtagactgc gctgcgcatg gcgcttgtct tttggttgca gcgcgggcgt aaagacttac
     4381 catgtgctgc ccgaaaggtc tttgttcttg cctgtgcgcg cagactttga ctatactctc
     4441 cccacagggc aggaaagtgc acgaaatctt tcatccggca gtcagtaatt actttgaacg
     4501 cacttttggt gccccgacgc cagtgcagct gaaggcatgg caggtgctcc acggtgaaaa
     4561 cagccggcag catgcactga tcagcgcacc cacgggcgag gggaagacgc tcgcggcgtt
     4621 tctggcaatc attgaccggc tggtcaggca ggcagctgcc ggggaagata ttggcaatac
     4681 gcggatactg tacatttcgc cgctcaaggc actggccaac gacatcgaga aaaacctgca
     4741 gcagccgctc gcgggaatac gcgcagagct ggcacgaatg ggcatcgaaa ttccggaaat
     4801 acaggcaatg gtgcgtacgg gtgacagctc gcagagtgaa cgggcacgcc tcgttcgcct
     4861 gcggccacag atcgttgtta ccacgcccga atcgttctac cttttactga caagtgaatc
     4921 gggccgacgc ctgctttcga caattgaaac agtgatcatc gacgagattc atgccctggt
     4981 cgccgagcgg cgcggagctc atctggcttt gtcgctggcc cgtctcgaag cattgcgccg
     5041 tgatcacgat gcgcattgcc ggccgctgca gcgtatcggg gtgtcggcga ccgtgcgccc
     5101 gctcgaacgg gtggctcagt accttgcggg caatgaccca gttcgcttct gtaccgatga
     5161 acgccgccgg ccattcgata ttcgcctgct cttgcccgac gaagggctca gtgccgtcat
     5221 gagcggcaac cattggaacc aaatatacct gaagctcacc gaagaaatcc gcaaccggcg
     5281 aacgacgctg gtctttgtta ataaccggcg attgtgtgag cgcatggcac gttcgctcag
     5341 cgagcttatc ggtgctgaat ttgtcggcgc ccaccacggt tcgttatcac atgcagagag
     5401 gcgccgcggt gaagagcgac tcaaggcggg atctctcaaa gtcatggttg cgacgcagtc
     5461 actcgaactc ggtctcgata tcgggtctat tgacctcgtg gtgcaggtgt cttcaccgcg
     5521 ttccattcat gccttcatcc agcgagcggg ccgttcagaa caccataagc aggggacctc
     5581 gcgaatcatt ttggtgccat tgacgcgcga cgatttgcgc gaatgcgtgg cgctggtctc
     5641 cgcactgcgc aggggcgaac ttgaagaagt aaagttatgt gctccagccc tcgatgtgtt
     5701 ggcgcaacag gttgttgcag aggtttctgc gcgcgactat gctgtcgatg agctgttcgc
     5761 gctgttcact ggctcttttg tctaccatga ccttcagcgg tcaaaatttc tgcagattct
     5821 gcagatgctg aatgacgggt tttccctgcg gttcggccgg cgctcccgct acctgttctt
     5881 cgaccagaca gcgggaacct tacatgcgcg acccggtgcg cggctcattg ctgctctgaa
     5941 tggaggggcg attcccgaga attttgagta cgaagtggtc gatgaaacgg accaggttta
     6001 tgtcggcagc gtgcatgaag attttgccat tgagtcgatg cggggcgatg ttttccagtt
     6061 gggcaaccgg ctgtggcaga tcgtgcaggt gagcggcaac cgagtctccg tcattgctgc
     6121 acccgacaag catccgacca ttccgttctg gattgtcgat gtgcctggca gatcggacac
     6181 tctgagcgct gctgtcgctc gcctgaacga ggattttctc gctgcagaat cagcggacca
     6241 tttcgtgacg gccatcgcca gggagacagg gttggaaaag cgccccctgg ttctgctcgc
     6301 cgattacctg ctgcaggcaa aaaattctct gggcagtctg ccgacgcagc agcaggttgt
     6361 cctcgaacga ttctttgacg aggtgcgcaa ctgccatatc gtgttgcact ccccctttgg
     6421 tgcgcaggtg aaccgcgcgt ggggatttgc actgcgcaag cgcttctgcc gcaagttcaa
     6481 tttcgaactg caggctgcg
//
LOCUS       DAOQJF010000186         6490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6657_length_6490_cov_4.682207, whole genome shotgun sequence.
ACCESSION   DAOQJF010000186 DAOQJF010000000
VERSION     DAOQJF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6657_length_6490_cov_4.682207"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PLF85_10435"
     CDS             <1..1120
                     /locus_tag="PLF85_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE20128.1"
                     /translation="STAEPPIVFYLGRLLGQRFKFMASRQVFTWQGGAIGWFIRRIGA
                     YSVIAGVPDRDAMKLTRKILSEPTGKLVIFPEGEPTSGEVDTLMPFQPGVAQLGLWGL
                     EDARKTDPAADINVWYAFMKFVIISPREKILHDLNKSLTRIEKKLGVAASNKDKHLLH
                     RFLTVGRLLLEQAERDFNVKPDVEKGFDYRIGRIRHAMLDALAEKLQAANYDQNEDAI
                     RKWRRLFALVELHQLKYPDPKLPKLSAKTVKEAHRDAVFIFDFIVIKRDYILDNPTPE
                     RLYEWLDRYESYMFKKVPRALGGVPSHLPRRAHILFGKPYKLSEIVTDKRSDRRAAVE
                     DFTNRLRQDMQKLLDQAQSLTQPLFTTEEILAVKSKIQ"
     gene            1130..1354
                     /locus_tag="PLF85_10440"
     CDS             1130..1354
                     /locus_tag="PLF85_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20129.1"
                     /translation="MQQQQNPGQLKHCSCGHDRHHPLVVRKNKYSGWGMLGLLTGISV
                     KPIFARYQCTKCGEFFDETNDPAELSKYLM"
     gene            1501..3249
                     /locus_tag="PLF85_10445"
     CDS             1501..3249
                     /locus_tag="PLF85_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE20130.1"
                     /translation="MIQGNYYDSNKDLQFTVDHFIDWATIVPLKEEGFADAAEYKKTG
                     DKHLEFAPSSTEETLEGYKAVWQQSGELSGIEIAGAARSMEKHGLKFNNGKVTFPPET
                     LRLVDLFAQSGLLGYSMGRHYGGLNMNLSASSIVMEMVSQADSAFGITLGCFNLAEVI
                     ERFGSKEMTAEWVPKMSAGEVTGAMALTEPNYGSDLSRVMTRAVKGEDGVWRLTGTKR
                     FITHGCGVGDRPAVILTLARSGGAGAKGLSFFLVHSSEIEVARIEEKLGLHTSPTCEI
                     VFENSKAELIGEEGLGLVRYAMGMMNGARMGIAVQSLGIAQAAHNEGKKYASERVQFG
                     DTIDKLPAVKRILEDNEALVQGMRALTYRACEVVDYYDGLSNKLLKETGDERAVRKNE
                     EVVRLDKLAKLLTPTAKLFCSEEANFVAYHSLQIFGGSGYTEEYDIAKIYRDARICTI
                     YEGTTQLQAVAAIGGIVEGMRENGFLLGHLRTEIAKLADVKTKADLTADVEQLVKLVP
                     QYKERAEKEAVSIDMVAAFARIYTSILLAQQLQVAKQKGLADIAEAKAKVFPHFHVMS
                     RRYLAGLAVTLASAAA"
     gene            3296..3694
                     /gene="queF"
                     /locus_tag="PLF85_10450"
     CDS             3296..3694
                     /gene="queF"
                     /locus_tag="PLF85_10450"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004766108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNE20131.1"
                     /translation="MTPTTPESYEGRQGHIRTLQLPAIEVFENIYPDREYNIHLDFPE
                     FTAICPKTGLPDFGVVEIDYTPDKYCLELKSLKEYFLAYRSVGIFHENVVNRILDDIL
                     LAADMRKAFVKVVYNARGGIVTTVSRSYER"
     gene            3694..4404
                     /locus_tag="PLF85_10455"
     CDS             3694..4404
                     /locus_tag="PLF85_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNE20132.1"
                     /translation="MVQTRPLWQRYLAVVAYLLLGFLIVQALATVTAGLTGIAENQGK
                     MPFRSLLFAPVIQMVGFLLPAPLLLRFTRAGNFGFARASAGDVLLACGLTFASLVVFS
                     LLYHALGIQPQQLAFLDQREILHHRQAFLIMTAVVVPAYEEWIFRGVIFGVLVTQYEN
                     RRSVFTAGIFTALLFTASHIEGKHSYSALPPIFAMALIFHYMTWRSGSIWPAVTAHAM
                     QNLLSATALIAKAGEQSR"
     gene            complement(4432..6081)
                     /locus_tag="PLF85_10460"
     CDS             complement(4432..6081)
                     /locus_tag="PLF85_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE20133.1"
                     /translation="MPPVSAAVQIFLNNQLVAAKGSVGPAMRYPHDSSEAFSWYPIKK
                     EFLGQQPEQLLELEITGFSGGGGLYGNSHIYFGGVEEIKIHYNQILFRTVFLAAAIFM
                     IALFHLALVPDTFHRRANLHYVLLALSMSAHILGMNGLGYLVWNQFLFNAGLIHLLVA
                     AFPFALTGFTLRYFRLQYPRIRRLAYYYGAAMSLFLIALAVFPQLVPLYLDFGLPFGV
                     AMMSASLAFVLFGALHGIRHGIEGAGLVFAGFLVYGLAVVNDVIFYFSYATPYKLADI
                     GFLVAVVSVALALAWRLRRSNREREELREWQKEITLAAHIQNQALPQRMLRTPHLQIA
                     TLFKPMKIIGGDFFAFHEISENETGVFIADVSGHGIAAALTVNTLRTVFHQFRDKARS
                     PAELLACMNSALYPHLHEQFVTAAYCLFDFAGNRLCLAQAGHPPVYLLKRNEASYDKV
                     KPKGRFFGFDREQAYEEAEYELSQYRRVFLYSDGVIEAGALKGNPYSVSRLENLLAIT
                     QDTQQGELLQLLDQDIREKTGTEMNTDDDSSCVVADLFAVA"
BASE COUNT         1632 a         1846 c         1678 g         1334 t
ORIGIN      
        1 gtcgactgcc gaaccaccga tcgtgttcta tctcggccgc ctgctggggc aacgcttcaa
       61 gttcatggcc tcacgccagg tgtttacctg gcagggcggc gcgattggct ggtttatacg
      121 gcgcattgga gcgtactcgg taattgcggg cgtacccgac cgcgacgcca tgaaactgac
      181 gcgcaaaatt ctcagcgagc ccacgggcaa actcgtcatc tttcccgaag gtgaacccac
      241 ttcaggcgaa gtcgatacgc tcatgccgtt ccagcctgga gttgcacaac tgggactctg
      301 ggggcttgaa gatgcccgta agacagaccc ggcggcagac attaacgtct ggtatgcatt
      361 catgaaattc gtgattatat caccgcgcga aaaaattttg catgacctga ataaatccct
      421 cacccgtatc gaaaaaaagc tcggtgtcgc cgctagcaac aaagataaac acctgctgca
      481 ccggtttctg accgtaggca ggctcttgct cgaacaggcg gagcgcgact tcaatgtgaa
      541 accagacgta gagaaaggct ttgactaccg catcgggcgc atcaggcacg cgatgctcga
      601 cgccctcgcc gaaaaactgc aggcggcaaa ctacgaccaa aatgaagacg cgatccgcaa
      661 atggcggcgg ctctttgccc tcgtcgagct gcaccagctc aaataccctg accccaaact
      721 gccgaagctg agcgccaaaa ccgtcaaaga agcccaccgc gatgcggttt ttatattcga
      781 cttcatcgtt atcaaacgcg attacattct cgacaacccg acacccgaac gcctctacga
      841 atggctcgat cgttacgaat cttacatgtt caagaaagta ccgcgcgcgc tgggcggagt
      901 tccctctcac ctgccgcgcc gggcccacat cctgtttggc aaaccctaca agttgtcaga
      961 aattgtgacc gataagcgca gcgaccgtcg cgccgcagtc gaagacttca ccaaccgcct
     1021 gcggcaggac atgcagaaat tactcgacca ggcgcagtcg ctgacccagc ccctgttcac
     1081 caccgaggaa attcttgctg tgaagtctaa gattcagtaa cctactgaag tgcaacagca
     1141 acaaaacccc ggccaactga aacactgctc ctgcggacac gatcgccacc acccgctggt
     1201 agtgcgcaag aacaagtatt caggctgggg catgctgggg cttttgaccg gcatttcggt
     1261 aaaacccatt ttcgcgcgct accagtgcac gaaatgcggc gaattcttcg acgagacaaa
     1321 cgatccggca gaactgagca aatacctgat gtgaccagcg gcgcgccgct catgggcaaa
     1381 agccggctgc atctaccttg acgctctgtc tcgtacaccc gatgcgtcga agattatcga
     1441 cgcgttgttc ggcctgaaaa cgtgcacata cacgttcagg aaggcagccg ggagggtcaa
     1501 atgatacagg gaaattatta cgatagcaac aaggatctgc agttcacggt agatcatttc
     1561 atcgactggg cgactattgt cccactcaaa gaagaaggtt tcgccgatgc ggcggaatac
     1621 aaaaaaaccg gtgacaaaca cctcgaattc gctccctcca gcacagaaga aacactcgaa
     1681 ggctacaagg ccgtctggca gcagtcgggc gaactctcgg gcatcgaaat tgccggcgcg
     1741 gcaagatcga tggaaaaaca cggtctcaaa ttcaataacg gcaaggtgac ctttcccccg
     1801 gaaacgctac gcctggttga cctgttcgca cagtcaggcc tgctcggtta ctcgatgggt
     1861 cgccactacg gcggtctgaa catgaacctg agcgcctcat cgatcgtgat ggaaatggta
     1921 tcgcaggcag actcggcttt tggcatcacg ctcggctgtt ttaacctggc cgaggtaatc
     1981 gagcgttttg gcagcaaaga aatgactgct gaatgggtac ccaaaatgtc agccggcgaa
     2041 gtgaccggcg ccatggcgct gactgaaccc aactacggtt cagacctgtc acgcgtcatg
     2101 acacgcgcgg taaaaggcga agacggcgtc tggcgcctca ccggtacaaa acgcttcatc
     2161 acgcacggct gcggcgtggg cgatcgtccc gcggtgattc tgacgctggc gcgcagcggc
     2221 ggtgccggtg caaagggatt gtctttcttt ttggttcaca gctctgaaat cgaagtggcc
     2281 cgcatcgaag agaagctggg cctgcatacc tcaccgacct gcgaaattgt ttttgaaaac
     2341 agcaaggcag aactgatcgg cgaagaaggc ctggggctcg tccgctatgc tatgggcatg
     2401 atgaacggtg cgcgtatggg tatcgctgtt caatcgcttg gcattgcgca ggccgcgcac
     2461 aatgaaggta aaaagtatgc ttctgagcgc gtacagttcg gcgacacgat cgacaaacta
     2521 cccgcggtga aacgcattct tgaggacaac gaagccctcg tacaaggtat gcgcgccctt
     2581 acctaccgcg cctgcgaggt ggttgactac tacgacggtc tgtcgaacaa gctgcttaaa
     2641 gaaaccggcg acgagcgcgc agtacgtaag aacgaagaag tagtccgcct tgacaagctc
     2701 gcgaaactgc tcacaccaac tgctaaactg ttttgctctg aagaagccaa cttcgtggcc
     2761 taccactcac tgcaaatctt cggtggctct ggttacacag aagagtacga catcgccaag
     2821 atttaccgcg atgcccgtat atgcacaatc tacgaaggta ccacgcagct gcaggccgtt
     2881 gctgcaattg gtggtatcgt agagggcatg cgggaaaacg gcttcctgct cggccacctg
     2941 cgtacagaaa tagccaaact tgccgatgta aaaacaaaag ctgatcttac agcggatgtc
     3001 gagcagctgg tgaaactggt tccgcagtac aaagaacgcg ctgaaaaaga agcggtctcg
     3061 atcgacatgg tggcagcttt tgcccgcatc tacacgtcga ttctgctggc gcaacagctg
     3121 caggttgcaa aacagaaggg cctggcagac attgccgaag caaaagcgaa agtgttcccc
     3181 cactttcacg tgatgagccg ccgttacctg gcgggcctcg cggtgacgct ggcgtcagcg
     3241 gcagcctgag aaacagagta aagccggggg ctctcccccg gtttccagaa ccaccatgac
     3301 tcccacaacc ccagaatctt atgaaggccg ccagggccac attcgcaccc tgcaattgcc
     3361 ggcgatcgag gtattcgaga acatttaccc ggaccgcgaa tataacatac atctcgattt
     3421 tcccgaattc acggcaattt gcccgaagac tggcctgcct gatttcggcg tagtcgaaat
     3481 cgattacacg ccagacaagt actgtctcga gctgaaatcg ctgaaagaat actttcttgc
     3541 ctaccgctca gtcggtatct ttcatgaaaa cgtcgtcaac cggatactcg acgacatcct
     3601 gctcgcggca gatatgcgca aagctttcgt gaaagttgtc tataacgccc gcggcggaat
     3661 tgtcaccact gtcagccgct cttacgaacg ctgatggtac aaacccgccc gctctggcaa
     3721 cgctacctcg ccgtagttgc ttaccttttg ctgggatttc ttattgtaca ggcgctcgcc
     3781 acggtgactg cgggcctcac cggtatcgca gaaaaccagg gcaagatgcc ctttcgctcg
     3841 ctgctttttg cgccggtaat ccagatggtg ggatttctac tgccagcgcc tcttttgctg
     3901 cgttttaccc gcgccggtaa ttttggcttc gcgcgggcaa gtgccggcga tgtgctgctc
     3961 gcctgcgggc ttacctttgc gagcctcgtg gtcttcagcc tgctttacca tgccctgggc
     4021 attcaaccgc agcaactggc ctttctcgac cagcgtgaga ttctgcacca ccggcaggca
     4081 tttctcatca tgactgcggt cgtggtgccc gcctacgaag aatggatttt ccggggagta
     4141 atttttggtg tgctggtcac gcagtatgaa aaccggcgct ccgtttttac tgcaggtatt
     4201 ttcactgccc tgcttttcac cgccagccac attgagggca agcacagtta cagcgcattg
     4261 cctccgattt ttgccatggc gctgatcttc cattacatga catggcgctc gggaagcatc
     4321 tggcctgccg tcaccgctca tgcaatgcag aacctgcttt ccgcaactgc cctgatagcg
     4381 aaggctggcg agcaaagccg gtaaaagcag ggggagatta tcttttactg gtcaggcgac
     4441 cgcaaacagg tctgccacca cgcacgagct gtcgtcgtcc gtgttcatct cggtgccggt
     4501 cttctccctg atatcctgat cgagaagctg aagaagctcc ccctgttgcg tgtcctgcgt
     4561 tatggccaac aggttctcca atctgctcac cgaataagga ttgcccttaa gcgcaccggc
     4621 ctcgataacg ccatctgaat agaggaaaac acgccgatac tgactgagtt catattctgc
     4681 ctcttcgtaa gcctgctcgc ggtcaaagcc aaagaacctg cccttgggct tgaccttgtc
     4741 ataactggct tcattccttt tcagcaaata gaccggtgga tggccggcct gcgcgaggca
     4801 gaggcggtta cccgcaaagt caaacaggca ataggccgcg gtgacaaact gctcgtgcag
     4861 gtgcggatag agcgcactgt tcatgcaggc cagcagctcg gcaggcgacc gggctttgtc
     4921 gcgaaactgg tgaaaaactg tgcggagtgt attcacggtg agggctgcag caataccatg
     4981 gcctgatacg tcagcgataa aaacgccggt ctcattttcg gatatttcgt ggaaggcaaa
     5041 aaaatctccc ccgataatct tcatgggttt gaatagcgta gcgatttgca aatgcggagt
     5101 ccttagcatc cgttgcggca gcgcctgatt ctggatatgc gctgcgagcg tgatctcttt
     5161 ttgccattca cgcaattctt ccctctcacg gtttgacctc cgcagccgcc aggcaagcgc
     5221 cagggcgacg gaaacaacag caaccaggaa accgatatcc gcaagcttgt agggagttgc
     5281 gtaggaaaaa taaaaaatga cgtcattcac gacggccagg ccatacacga gaaaacctgc
     5341 aaaaacaagc ccagcaccct caataccatg gcgtatacca tgcagtgcgc caaacagcac
     5401 aaaagccagt gacgccgaca tcatcgcgac gccaaagggc agaccaaaat ccagataaag
     5461 tggcaccaac tgcggaaata ctgccagtgc tatcagaaac agcgacatag cagcgccgta
     5521 ataatatgcc agcctgcgga tgcgcgggta ttgcaggcga aaatagcgca gggtaaaacc
     5581 tgtcagggcg aaaggaaacg ctgcgaccag caggtggatc aacccggcat tgaagaggaa
     5641 ctggttccag acaaggtacc ccaatccatt cattcccaga atatgcgccg acatcgacag
     5701 agccagcagg acataatgca ggtttgcacg gcgatggaat gtatcgggaa caagtgccag
     5761 gtggaagagc gcgatcatga aaattgccgc ggcaagaaaa acagtacgaa acagtatctg
     5821 gttatagtgg atcttaattt cttccactcc tccgaaatag atatgcgagt ttccgtacag
     5881 cccgcccccg ccactgaagc cggtaatttc caactcgagc agctgttccg gttgctgccc
     5941 taaaaattct ttcttaatcg ggtaccagct aaaagcttct gaagaatcat gcggatagcg
     6001 catagcaggc cccacgctgc cctttgccgc tactaactgg ttattgagaa atatctgcac
     6061 ggcagcagag acagggggca atatgaggcc aaagtctcct gaccgcaagg actcggaaat
     6121 actcaactgt agcgagaacc tggctctctc aaccccctga atgccctgca gccgccattc
     6181 tttatcaact ctgagtttac cctgatgatt acccgccttg aaattccaaa aaccgttcag
     6241 cggagtgaca gcacgatcag gaagcacgac cttcgcatca tccacgaacc cgtgcagatg
     6301 aaagcccaac aacaaagtta gcaggcaaaa agcgcgcacc gaatttccgt ctgtgccaca
     6361 aatacccaag cgtaaaggaa aaaaaattgc atcaccccaa aagcatcagg gtatgccccc
     6421 tagtctatcg ctagagatct cccaacaaaa aaggccccaa cgggtctgta tttgtacgcc
     6481 aacccacttc
//
LOCUS       DAOQJF010000187         6472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6688_length_6472_cov_4.333801, whole genome shotgun sequence.
ACCESSION   DAOQJF010000187 DAOQJF010000000
VERSION     DAOQJF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6688_length_6472_cov_4.333801"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLF85_10465"
     CDS             complement(<1..746)
                     /locus_tag="PLF85_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20134.1"
                     /translation="MLLPLARLSLLAAALVSLRPVASAPQQVNSPITAARAQCTPAGE
                     AAKNCQLRLLVQPYAPAVFTLGGKELKARFSKQDKTLSQAELTLMNRPTREDSLLLTV
                     TDKKTGGSRLVTVALSADKVAETPLVFLDNPAARHRFVSRIATGKQPKSAMFISDRQV
                     ILPLLDDNHLEVIDIVTGQTRRLELPAQYAKRGGFVESVRVPTRGEFWVSQMKAAAIH
                     RFAADTLEYRGTIHVSGAWTKVLAYDAVRNL"
     gene            871..1071
                     /locus_tag="PLF85_10470"
     CDS             871..1071
                     /locus_tag="PLF85_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNE20135.1"
                     /translation="MIELSRLNGKVFYLNHNLIEIFESTPDTVIKLTDGTKYIVREKP
                     DELLAKIVAFNRQVFAEKTRIE"
     gene            1183..1719
                     /locus_tag="PLF85_10475"
     CDS             1183..1719
                     /locus_tag="PLF85_10475"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNE20136.1"
                     /translation="MADAEDLDQDDAGEEAVAAPMGGGRSRIVTILIWVVAGLIALAL
                     MFVISVMVAKYYKNADFQETESITIAPPSAPPAIFKIAHEFRVNTADTDEAHYAQAVI
                     TLGYEGEVNKQLDAELAKRLPQVIHIINIILGGKRKDELFTPLQKLNLGEEIKNQINM
                     ILRDGKISEVMFEQLVVS"
     gene            1759..>2446
                     /locus_tag="PLF85_10480"
     CDS             1759..>2446
                     /locus_tag="PLF85_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20137.1"
                     /translation="MGDGSLSQEEIDALLMGTDDMAAPAGKAGGVATSDGGGLSPTER
                     ELLADAFNDAMQVAGAQAGAMVGKNVQIANAQVDEISPGQLSAELPKGGVLAEIMMGP
                     TQTVLAFPKDLARRIAQTMMGATDDGGEINDAHLSTLSELSQSIISSIANGLGAKFND
                     SLSPSMPDMKIFNGPGDTPQFPGSIVKISYNMSVEGIPGSRVVHYIDGGSASRWAKAN
                     RGSAAPQAASF"
     assembly_gap    2447..2546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2800..3246)
                     /locus_tag="PLF85_10485"
     CDS             complement(2800..3246)
                     /locus_tag="PLF85_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20138.1"
                     /translation="MPRLAASLIAALVLLRCGTSIPFDQELFLKRPTGLQVTAVAGQK
                     MLVQYTVQNQEETFDGYNLLISRISIGDSEAFSMEPLTINGSIPTFLHSKSEFNVNTV
                     RSVTIERFTNVLPFEIGTTYYFRLQAHSRKGVRSEASNEVTATGIP"
     gene            complement(3309..4295)
                     /locus_tag="PLF85_10490"
     CDS             complement(3309..4295)
                     /locus_tag="PLF85_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE20139.1"
                     /translation="MRRALLLLAFLALSLFYAGAYCRQANLKYTYTPKPLAGDFKTFY
                     ERNRNFSLRAGVPERATERLVINNPEKDGIAILYIHGFGASRAEGEYTVDALAQAISA
                     NVYYLRLPGHGTTAEAHAQATFDQYLSTAEEALLHMPLLGKKTVVIGTSTGALIATYL
                     ASRYSDRMHAVIFASPLWDFANKTTRLLNFPGGLALGQMVMGTERDAGWKSDPENRKH
                     ADYEKHWMIKQKFAALVNLNNLRRFVVKPETFAAIKVPALALVYYKDDKHQDEAIDVA
                     KVREVMPQLGSAAGGKNRLIEVADGNHILLSEFVRTDKATQLREIRAWLSGL"
     gene            4346..>6472
                     /locus_tag="PLF85_10495"
     CDS             4346..>6472
                     /locus_tag="PLF85_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNE20140.1"
                     /translation="MPAPKKSSKQKTKLVLIDGHAMAYRAHFAFAGQNLTDSAGNATE
                     TTFGFYRMVARLIADRKPTHVVLCFDPGREHNPRYELYPDYKANRAAMPDELRRQIDD
                     IQKMAGELHLPPLIVEGAEADDTIASIIEQHKDDFDAIEIVSGDKDLYNMLYKNVVLL
                     RSKKGVTEFVEIDEAWVKKEIGITREQVPDYMALVGDTSDNVPGVKGVGEKTAAKLIG
                     EYKSLEKIYKNLDKVKPDGLRAKLEVSRDNAFTSQKLVTLRKDIPLAVKPADMVLQLN
                     AEGGNIFRKRGFTTVASEWEKLLKKAGGSTALTNQPDAAEEKMRSLSAVEASNEIKFC
                     QQTQTITTVAGLKTLLATLNKLEEVCVDTETTGLDIHDSEMFGVSFGWSAGKELYSAY
                     VALPDGGEAAMNGDYADADGAAKLLPLLRDFLENKKIHKIGQNIKFDIQVFRRAGIEV
                     ANYAEDTMLMSYLLNPNIRRHNMDDMAEDHLHYKTVTFDELVGKGKGRLPIGAVPLDR
                     LSHYACEDAEVTLALRDVLLPKVKAASLEKVYQEIDVPLARVLADIEYTGIAIDVQHL
                     AELGKEYKKKIAAAEKLIYKHAGEEFNIASTKQLQNVLFEKLQIASGRKTEKGALSTD
                     QGVLEELRDAHPIIAPILDYRFYTKLLSTYVEALPGHVSKHTGRVHTSLSQVTAATGR
                     LSSIDPNLQNIPVKGEEGAHLRAAFVA"
BASE COUNT         1442 a         1759 c         1895 g         1276 t
ORIGIN      
        1 agattgcgca cggcgtcata tgccagtacc ttggtccagg cgcctgacac gtgaatggta
       61 ccgcggtact cgagcgtatc ggcagcgaag cggtgtatgg cggccgcctt catctggctc
      121 acccagaact cccccctggt gggtactcgc acgctttcga cgaagccgcc gcgtttggcg
      181 tactgcgcgg gcagttctag ccggcgtgtc tggcctgtga caatgtcgat cacctcgagg
      241 tggttgtcgt cgagcagtgg caaaatcacc tgccggtctg aaatgaacat cgcgctcttc
      301 ggctgcttgc cggtcgcaat acgcgagacg aagcggtggc gggcagccgg gttatcgaga
      361 aacaccagcg gcgtttctgc caccttgtcg gctgacaggg cgacggtcac gagccggctg
      421 cccccggtct tcttatcggt cacggtgagc aagaggctgt cctcgcgggt agggcggttc
      481 atcagggtaa gctcggcctg cgacagcgtc ttgtcttgct tcgagaaccg ggccttcagt
      541 tctttgccgc cgagggtaaa gacggcaggg gcgtacggct gcaccagcaa cctgagctgg
      601 cagttctttg ctgcctcacc ggcaggggta cactgcgcgc gggctgcggt aatgggggag
      661 tttacctgct gcggggcgct ggctaccggg cgcaggctga ccagtgctgc ggcaagaagg
      721 ctgagacgag cgagaggaag aagcataagg taataccacg attccccgcg gcgcggaaca
      781 gaattattcg ctttaaggaa gcgccgggaa atccgacata atgagcaggg tgtgcggggt
      841 ttccggcgca cccgaacgcg gtaaaaagca gtgattgaac tcagcaggct caatggcaag
      901 gtgttctacc tgaaccataa cctgatcgag atattcgaat cgacgcccga caccgtgatt
      961 aagctcacag atggcacgaa atacatcgtg cgcgagaaac ccgatgagct gctcgcaaag
     1021 atcgtggctt ttaaccggca ggtatttgcc gagaagacgc ggatagaata gtttcccgaa
     1081 aggcagcggc gcttgcagcc gccagcgggg ttcttaaggg gatacgcagt atccccttaa
     1141 actacaaatg ggtgccgggc taagcccggc acggaggttg agatggcaga cgcagaagac
     1201 ttagaccagg acgacgccgg tgaagaagcc gtcgccgccc ccatgggtgg cggcaggtcg
     1261 cgtatagtaa caatactcat ctgggtggtt gcgggcctta tcgccctggc gctgatgttt
     1321 gtcatttcgg tgatggtggc gaagtattat aagaatgccg acttccagga aaccgagagc
     1381 atcacgattg ctccgccgag cgctcccccg gcaatcttca agattgcaca cgagtttcgt
     1441 gtgaacacgg cagataccga cgaagcgcac tacgcgcagg cggtaattac actcggttac
     1501 gagggcgaag ttaacaagca gctcgatgca gagctggcga agcgcctgcc gcaggtgatt
     1561 cacatcatta atatcatcct tggcggtaaa cgcaaggatg aacttttcac gccgttgcag
     1621 aaactgaatc tgggcgaaga gatcaagaac cagatcaaca tgattctgcg cgacggcaaa
     1681 atttctgaag taatgttcga gcagttggtg gtaagttgat ggttaatttc ccgcagggaa
     1741 attaaccacg gaggcaaaat gggtgacggt tcactttcgc aggaagaaat agacgcctta
     1801 ttgatgggca cagacgacat ggcggcgcct gccggtaaag caggtggagt tgccacgtct
     1861 gacggcggcg gcctgtcgcc gactgaacgc gaacttctcg ctgacgcttt caacgacgcg
     1921 atgcaggtcg cgggcgccca ggcgggcgct atggtcggca agaatgtcca gatcgccaac
     1981 gcgcaggtag acgaaatttc tcccggccag ctctctgcag aactgcccaa gggcggcgtg
     2041 ctcgccgaga tcatgatggg gccgacccag acggtactcg cgttcccgaa agacctggca
     2101 aggcgcatcg cgcagaccat gatgggtgcg acagacgatg gcggcgaaat taacgacgct
     2161 cacctctcta cgctttctga gctctcacag agtatcatct cttccatcgc taacggcctt
     2221 ggcgcaaagt tcaacgattc gctctcgcct tcgatgcccg atatgaagat cttcaatggt
     2281 ccgggcgata caccgcagtt tccgggttcc attgttaaga tatcttacaa catgtcggtc
     2341 gagggtatac cgggttcgcg cgtggtacac tacatcgacg gcggttcagc ctcgcgctgg
     2401 gcaaaagcca accggggctc agcagctcct caggccgctt catttgnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnncgaa aacttcggcg tgcgcgtaac cgacatcgtc
     2581 ggccctgccg aacgtatggc aaggatggct ggggggtaat cacccctgac aaacgcacgg
     2641 gcagtctgcg gaaaaaattc cgcaaaagcg aaaagcctcg tcgtttgccc tcgcaaacga
     2701 ctgctaaagc gcgtcgtcgc ttgcaaagcc aagcgacttt gctgcttttc gcttttacgg
     2761 tttccgcaga ctgctcgcag tttccttttc aggaaactgc taaggtattc ccgtagcggt
     2821 cacttcgttc gaagcctctg accgcactcc cttacgcgag tgtgcctgca ggcgaaagta
     2881 ataggtggtg ccgatttcaa acggcagaac attcgtaaac cgctcaatcg ttacgctgcg
     2941 caccgtattt acattaaatt cgctcttcga atgcaggaag gtgggaatgc tgccgttgat
     3001 tgtcagcggc tccatgctga aggcttcgct gtcgccgatg ctgatgcgcg agatcagcag
     3061 gttgtagccg tcgaaggtct cttcctggtt ctgcaccgtg tactgcacga gcattttctg
     3121 accggcgaca gctgtcacct gcagacccgt ggggcgtttg agaaatagct cctggtcaaa
     3181 gggtatactg gtgccgcagc gtaagagcac aagagcggcg ataaggctgg cagcaagacg
     3241 cggcatggac ttgtcaggct cgcagaacct gtttcaggcg gtcaaacaga atgaagatcc
     3301 attcgctcct ataatccgct aagccaggcg cgaatctcgc gcagctgtgt tgccttgtcg
     3361 gtgcgcacga attcactcag cagtatgtga ttgccgtcgg caacttcgat caagcggttc
     3421 ttaccgcctg ccgcgctgcc gagctgcggc ataacctcgc gcaccttggc cacgtcgatg
     3481 gcttcatcct ggtgtttatc atccttgtag tacacgagcg ccagcgccgg taccttgatc
     3541 gcggcaaaag tttcgggttt aacgacgaag cgccgcaggt tattgaggtt caccagtgcg
     3601 gcaaatttct gtttgatcat ccagtgcttt tcatagtcgg cgtgcttgcg attttcaggg
     3661 tcgctcttcc agccggcgtc gcgttcggtg cccatgacca tctgcccgag cgccagaccg
     3721 ccgggaaagt tcagcagccg cgtggtcttg tttgcgaaat cccacagcgg agaagcgaag
     3781 atcacggcgt gcatacggtc gctgtagcgt gaggcgaggt acgttgcgat cagcgcaccg
     3841 gtgctggtgc cgatcaccac ggtcttcttg ccgagcaggg gcatgtgcag cagcgcttct
     3901 tcggcggtgc tgaggtactg gtcaaaagtc gcctgagcat gcgcttcggc ggttgtgccg
     3961 tgaccgggca gccgcaggta gtagacattg gcgctgatcg cctgggcgag cgcatcgacc
     4021 gtgtattcgc cttcggcgcg gctcgcgcca aaaccatgga tatacagaat ggcaatgccg
     4081 tctttttcag gattgttgat gaccagccgc tcggttgcac gctccggcac gcctgcgcgc
     4141 agtgagaagt tgcggttgcg ttcgtagaag gttttgaaat cccccgcgag gggtttgggc
     4201 gtataggtat acttcaggtt ggcctgacga cagtacgccc ccgcataaaa cagcgagagg
     4261 gcgagaaatg ccagaagcag aagagcgcgg cgcatggtct atttgcactt gccgtgtgag
     4321 atcggcgagc ttttttaacc cggtcatgcc cgccccgaaa aaaagctcca agcaaaaaac
     4381 caagctcgtc ctcatcgatg gccatgcgat ggcgtaccgg gcacattttg cctttgccgg
     4441 gcagaacctg accgatagcg cgggcaacgc caccgaaacg acgttcggct tttaccgcat
     4501 ggtggcgcgt ctcattgccg atcgcaaacc gacgcacgtg gtactctgtt tcgaccccgg
     4561 ccgcgagcac aacccgcgct atgagctgta tcccgattac aaggcgaacc gcgcagcaat
     4621 gcccgacgag ctgcgccggc agatcgacga catacaaaaa atggcggggg agcttcacct
     4681 gccaccgctc atcgtcgaag gtgcagaggc cgacgacacg atcgcttcaa ttattgagca
     4741 gcacaaggat gattttgacg ctatcgaaat tgtttcgggc gataaagacc tgtataacat
     4801 gctctacaag aatgtcgttc tgctgcgctc caagaaaggg gtgaccgagt ttgtcgaaat
     4861 cgacgaggcc tgggtcaaaa aagagatcgg catcacccgc gagcaggtgc ccgactacat
     4921 ggcgctggtc ggcgatacgt cagataacgt gcccggcgtg aaaggcgtgg gggaaaaaac
     4981 tgctgcgaag ctgattggcg agtacaagtc gctcgagaaa atctacaaga acctcgataa
     5041 agttaaaccc gatggcctgc gggccaaatt ggaggtaagc cgcgacaatg ccttcacttc
     5101 gcagaagctt gtcactctgc gcaaagatat tccgctggcg gtgaagcctg ccgacatggt
     5161 gctgcagctc aacgccgaag gtggcaacat tttccgcaaa cgcggattca ccaccgttgc
     5221 cagcgagtgg gaaaagctgc tgaaaaaggc tggtggttcg actgcgctca ccaaccagcc
     5281 agatgccgca gaagagaaaa tgcgctcgct gagcgcagtc gaagcgagca atgaaatcaa
     5341 gttctgccag cagacacaga cgatcaccac ggtcgcaggg cttaagacgc tgctggccac
     5401 gttaaacaaa ctcgaagagg tctgtgtcga caccgagacc accggcctcg acatacacga
     5461 cagcgagatg tttggtgtct cctttggctg gtctgcaggc aaagaactgt acagtgccta
     5521 tgttgcgctg cccgacggcg gtgaagccgc gatgaacggc gactacgccg acgccgacgg
     5581 cgcggcaaaa ctgctgccgc tactcaggga tttccttgaa aacaagaaga tccacaagat
     5641 cgggcagaat atcaagtttg atatccaggt gtttcgccgc gcgggcatcg aggtggcaaa
     5701 ctatgccgaa gacacgatgc tgatgtctta cctgttgaac cccaatatac gccggcacaa
     5761 catggacgac atggcagaag accacctgca ctacaagact gtcacgtttg acgagctggt
     5821 cggcaaaggt aagggcaggc tgccgatcgg cgcggtgccg ctcgaccggc tctcacacta
     5881 tgcctgcgaa gatgccgagg tgacactcgc gttgcgcgat gtgcttctgc ccaaggtgaa
     5941 ggctgcttcg cttgaaaaag tctaccagga gattgacgtg ccgctggcca gggtactcgc
     6001 cgatatcgag tacacaggca ttgcaatcga cgtgcagcac ctggccgagc ttggcaaaga
     6061 gtacaaaaag aagattgccg ctgccgaaaa gctgatctac aaacacgcgg gcgaagaatt
     6121 caacatcgcc tcgacaaagc agctgcagaa cgtgctgttt gaaaaactgc agattgcttc
     6181 gggacgcaag acggagaagg gggcgctttc cactgaccag ggcgtgctcg aagagctgcg
     6241 cgatgcgcac ccgatcattg cgccgattct cgactaccgc ttctatacga agctgctcag
     6301 tacctatgtc gaggcactgc cagggcatgt gagcaagcac acgggtcgcg tgcacacctc
     6361 gctttcgcag gtgaccgcag ctaccggcag gctttcgagc attgacccca acctgcagaa
     6421 cattccggtc aaaggcgaag agggggctca cctgcgcgcc gcgtttgtgg cc
//
LOCUS       DAOQJF010000188         6454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6723_length_6454_cov_5.578840, whole genome shotgun sequence.
ACCESSION   DAOQJF010000188 DAOQJF010000000
VERSION     DAOQJF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6723_length_6454_cov_5.578840"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLF85_10500"
     CDS             complement(<1..735)
                     /locus_tag="PLF85_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20141.1"
                     /translation="MVTAQQLPPVLLVAGEESGDLLGAAIVREVLKKNKLPLWGCGGQ
                     RLREVGMDVRFTSEELSTIGFFEAAKNYRRLTKIMDKLVAEAAANGTREAWLIDFPGF
                     NIMLAKKLKDIGIRCTMIVSPTVWAWKYKRVFKIRDRFERVLCLYDFEPDIYNKIGVE
                     AHYIGHPLTAETVNLQAKIARSGNPVKKVPQLREVFRRKMPIIAVMPGSRPAEVQYHT
                     ARIIEGIRLFAHEHPGYAFVIPAANSK"
     gene            complement(743..2254)
                     /gene="purF"
                     /locus_tag="PLF85_10505"
     CDS             complement(743..2254)
                     /gene="purF"
                     /locus_tag="PLF85_10505"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNE20142.1"
                     /translation="MKRQKSTSAGQKNPKYYPKEECGIVGVYNVEEAANHAYLGAYAL
                     QHRGQESAGIVSSDGERLYRFAGMGKVAEVFTPQKIKQLTGRHAIAHNRYSTTGASFL
                     RNAQPIRFESRLGTMALAHNGNLTNAATIRRNLEDLGSLFQTTIDTEVISHLVARSRA
                     ENFTEALIEAVQQIRGAYSLVVLTGDTLYALRDPNGFRPLVMGKKDDGFVFASETCAL
                     DIIDAEYVRDVEPGELVIVTPRGITSQFPFGKSTQNLCIFEYVYFSRPDSFVFGKSVY
                     DTRLRMGEVLAEESPVEADLVMPVPDSSSVAALGYARKSGLPYHMGLIRSHYIGRTFI
                     EPDQKIRDFGAKIKYNAIGSVVKGKRVIVIDDSIMRGTTTRKIVKMLRHAGAKEIHVR
                     IASAPTRHACYYGIDIPHHSELIAATHTIDEITKYLRVDSLAYLSLEGMQRAAAETPG
                     GIGAGGAAVADHGNAGYCTACFDGKYPVRLDENEAIYSNQKTLFSEYEIEETA"
     gene            complement(2303..3376)
                     /locus_tag="PLF85_10510"
     CDS             complement(2303..3376)
                     /locus_tag="PLF85_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20143.1"
                     /translation="MRRMSLILLTAGVTLTPIAAALKKVIILPIINIDKDQNFAYLEA
                     SITDSLKERLREKFAFEELAEEKWALVAEQNFILRDDFHTKTAAMNLGILANQDIVIS
                     GGFKPVNKKLAGGKIKSSIHATAHLLDVKKKKAIATIELDLPADSELFTAVGQVADRM
                     EQETRKVIPSKEEAARTGLKAESEPFFADWSLGLRGGGGVYVSGYAKYFTAQLPALGA
                     TLHAAMPKFGKMTTEMGFIFMGHRLKEGNDSAIQSLGASGVTSNYMASLSFGYRIGLT
                     TKLYLEPQVGGGYVMQTTTVTGSGINSTLTNGFPFARGGIVTGYRINQNIDASLSLES
                     LAYIEQGVMTYVPLALAGFHYKF"
     gene            3526..4119
                     /locus_tag="PLF85_10515"
     CDS             3526..4119
                     /locus_tag="PLF85_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20144.1"
                     /translation="MQIIDSFEYNHTSISVRPNSHRRLKAAVKIARRAGIEWSESEIL
                     RRLAKLYLQAWRGRGKKSATARRYNQPVQGARYKRIPWYVDKVLYAVLWERSVHSGES
                     VSRMLDFAIRHHLPQLMEAVLRNPYTRHVRAQRNASYWEARYNRRPHRRPELFITYSC
                     KTSENGPHGLEYRQKYEIIPKTGLSPAEILHLLRYAA"
     gene            4363..5208
                     /locus_tag="PLF85_10520"
     CDS             4363..5208
                     /locus_tag="PLF85_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE20145.1"
                     /translation="MFNKTSSPQSKGATLLFLALSFAMVAGTTASLKAQAAPAATPAA
                     AAPAPAGETAPLGAEGNKEAAAFAEAGSLNIFESKDPALWILMVLFLVALAIGAERLW
                     VLLHTKSANAELVRLVTERLSQNPATGEELAKDVSEAKFGVEGRIAAVTLKGWQHDED
                     TLQSYANAATVAERRYLSARLPVLSTLGNNAPFIGLLGTVLGIMKAFRDLAGAADAGP
                     QVVMKGISEALVATAMGLGVAIPCVMLFNYFSSAIKRKLSNSEEVTNIIVALRRATRS
                     GNKVV"
     gene            5210..5638
                     /locus_tag="PLF85_10525"
     CDS             5210..5638
                     /locus_tag="PLF85_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNE20146.1"
                     /translation="MAAKVGNDSGDEVIGDINVVPLVDIMLVLVIILMVTAEFTKYRT
                     VPIQLPKVNAVAMKREPHKVAITIKPNGKVYLGDKEIKDLAELGPRLVSAKKAQPDVA
                     VILRMEKDTKYSDMLKVLDDVKLSGISKVGLAVDSAKKGK"
     gene            5706..6395
                     /locus_tag="PLF85_10530"
     CDS             5706..6395
                     /locus_tag="PLF85_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNE20147.1"
                     /translation="MGSISKRSSRSGVVSYDGIRLSDGRFVYPGLSNTFENYETIPWI
                     SRNKALWLGGALLVLSIAITVVKIDWSPDDSELDKLAVTVDFGDVVQPFKKKAAKPRE
                     EVDEVFGNEFIKDKTPPDPNQEDPRIASAVNAAVGGATMPVDLSPEIRPQYTAAARNA
                     GIEGAVTLEIVISEDGKVLRAKPVGKKLGLGLDEAAAATYKAKRFKPSVGPAGKPIVV
                     KYYERVKFMLN"
BASE COUNT         1504 a         1759 c         1755 g         1436 t
ORIGIN      
        1 cttagaattg gctgccggta tgacaaacgc ataaccagga tgctcgtggg caaaaagccg
       61 gattccctcg ataatgcgcg ccgtatggta ctgcacctca gccgggcgcg aaccgggcat
      121 tacggcgatg atgggcatct tacgtctaaa gacctcgcga agttgcggta ccttctttac
      181 cgggttgcct gatcgggcaa tttttgcctg caggtttact gtttcggctg taagcgggtg
      241 gccaatatag tgtgcctcaa caccgatctt gttataaatg tcgggttcaa aatcgtagag
      301 gcaaagtacc cgctcaaagc ggtcgcgaat cttgaaaacc cgtttgtatt tccaggccca
      361 gacagtaggt gaaacaatca tcgtgcagcg aataccgata tctttcagtt ttttcgcgag
      421 catgatgttg aaaccgggaa aatcaatcag ccacgcctcg cgggtaccgt tcgccgccgc
      481 ctctgcgacg agcttgtcca tgatcttcgt gagcctgcgg tagtttttcg ctgcttcaaa
      541 aaaaccgatg gttgaaagct cttcagaagt gaagcgcacg tccataccta cttcgcgcag
      601 gcgctggccg ccacagcccc acagggggag cttgttcttt ttcagaactt cgcggacgat
      661 ggctgcgcca agcaggtcac ccgactcttc gcccgcgacg agtaatacag gtggtagctg
      721 ctgcgcagtt accacgacgc gtctacgcgg tttcttcgat ttcgtattcg ctgaagagcg
      781 ttttttggtt agagtagatc gcttcgtttt catcgagtct gaccggatat ttgccgtcga
      841 aacatgccgt acagtaaccg gcattaccgt ggtcggcaac agcagcaccg cccgcgccga
      901 tgccgcccgg ggtctcagct gcggcacgct gcatgccttc gagcgaaaga taggcaagcg
      961 aatcgaccct caggtatttt gtgatctcgt cgatcgtgtg cgtcgccgcg atcagttcag
     1021 aatggtgtgg tatatcgatg ccgtaatagc aggcatggcg tgttggggca ctggcaatgc
     1081 gcacatgaat ctcttttgcg cctgcatgac gcagcatctt gacgatcttg cgggtcgttg
     1141 taccccgcat aatactatcg tcgatgacaa tgacgcgctt gcctttgact acagaaccaa
     1201 tcgcattata cttgatcttt gcaccgaagt ctcggatctt ctggtcaggt tcgatgaagg
     1261 tacggccaat atagtgtgaa cggataagcc ccatatggta gggcaatccg cttttgcgtg
     1321 cataacccag cgccgcgacc gatgatgaat cgggtacagg catgacgagg tctgcttcga
     1381 ccggtgactc ttcggccagt acctcaccca tgcgcaggcg cgtatcgtag accgatttgc
     1441 cgaagacaaa cgagtcggga cgcgaaaaat agacgtactc gaagatgcag aggttctgcg
     1501 tcgacttacc aaagggaaac tgagaggtaa taccacgcgg cgttacaata acgagctcgc
     1561 ccggttcgac gtcacgcacg tactccgcat cgatgatgtc gagagcgcag gtttcggaag
     1621 caaagacgaa gccgtcgtct ttcttcccca tcacgagcgg cctgaaacca ttcgggtcac
     1681 ggagtgcata gagcgtatca cccgtcagca ccacgagcga ataagcaccg cgaatctgct
     1741 gtactgcttc aataagggct tcagtaaaat tctctgcgcg cgagcgggca accaggtgac
     1801 tgataacttc ggtatcgatc gtggtctgga acagtgagcc aaggtcttcg aggttgcgcc
     1861 ggatagttgc ggcgttggtg aggttaccgt tgtgtgccag cgccatggta ccgaggcgcg
     1921 actcaaagcg tatgggctgc gcgttgcgca ggaaactcgc gccggtggta gaataacggt
     1981 tatgtgcgat ggcgtggcgg ccggtcagct gcttgatttt ttgtggtgta aaaacctcgg
     2041 caaccttgcc catgccggca aagcggtaaa gccgctcgcc gtccgatgag acaatgcctg
     2101 cactttcttg cccgcggtgc tgcagggcgt aggcgccgag gtaggcatgg ttggcggctt
     2161 cttcgacgtt ataaacgccg acaatgccgc attcttcttt ggggtagtat ttcgggtttt
     2221 tttgcccggc agatgttgat ttctggcgtt ttatgtcgct caccgggttt ggttataaac
     2281 catgagcgac ctgagccgca aatcaaaact tgtaatggaa cccggccagg gccaggggta
     2341 cgtaggtcat cacgccctgc tcgatgtagg cgagcgactc aaggctaagc gaggcgtcaa
     2401 tattctggtt gatccggtaa ccggtcacaa tccccccccg tgcaaatgga aaaccgttgg
     2461 tcagggtcga attgatgcca ctgccggtta cggtagttgt ttgcatcaca taaccgccac
     2521 caacctgtgg ctcgaggtag agcttggtag ttaaaccgat acgataacca aagctaagcg
     2581 aagccatgta gttggaggtc actccggacg ctccgagaga ctgtatggca gaatcatttc
     2641 cttcttttaa ccggtgcccc ataaaaatga agcccatttc tgtggtcatc ttgccgaact
     2701 ttggcatcgc ggcgtgcaga gtagcgccga gcgctggcag ctgcgcggtg aaatatttcg
     2761 cgtaaccaga aacataaact cccccaccac cgcgaaggcc caggctccag tcggcaaaga
     2821 aaggctcgct ctcagcctta agaccggtgc gcgctgcttc ttctttgcta gggataacct
     2881 tgcgcgtctc ctgttccatg cggtcagcca cctggccgac tgctgtgaag agttcgctgt
     2941 cagcaggcag gtcgagttca atggtggcaa tcgccttctt cttttttaca tcgagcaggt
     3001 gcgcagtcgc atggatgctc gatttaatct tgcccccggc aagtttcttg ttcaccggtt
     3061 taaatccacc gctgatgaca atgtcctggt tcgcgaggat accgagattc atcgcggctg
     3121 tcttggtatg gaaatcgtcg cgcaagatga aattctgctc tgcaactaag gcccattttt
     3181 cctcagccaa ttcttcaaat gcaaattttt cgcgcagacg ttctttgagt gaatcagtga
     3241 tggatgcctc aagataggca aagttctggt ctttgtcgat attgataatc ggcaaaataa
     3301 tgactttttt cagtgcggcg gcgatagggg taagggtcac gccagcggta agcaaaatga
     3361 ggctcattct gcgcattgtc aaagagagtt cacagctcta tcacgtcaat ttgaaatagg
     3421 aagggctttg gggctgcacg ggcgggccta caggatgtta tacccataaa aaaacgacct
     3481 atcccatgag aaaatttcgc tgttttctgg catttaatat gtagaatgca gataattgat
     3541 tcctttgaat acaaccacac ttccatctct gtccgaccga atagccatag aaggctcaag
     3601 gcagccgtaa aaattgcacg gcgagcagga attgaatggt cggaatcaga gatcctgcgt
     3661 cggctggcaa aactgtatct tcaggcctgg cgcggtcgcg ggaagaaatc ggcgaccgca
     3721 cggcgctaca atcagccagt tcaaggagca aggtacaagc gcataccgtg gtacgttgat
     3781 aaggttctgt acgcggtctt atgggagcgc tcggtccatt ctggtgaatc tgtctcgcgc
     3841 atgctggatt ttgcgattcg gcatcatctc ccgcagctga tggaggctgt tttgaggaat
     3901 ccttatacga ggcatgtgcg tgcccagcgg aatgccagct actgggaggc ccggtataac
     3961 cggcgacccc ataggcgtcc agaactgttt attacctact catgcaaaac aagtgaaaac
     4021 ggcccgcacg gattggagta ccgccagaaa tacgagataa ttcccaaaac aggactctca
     4081 cccgcagaaa tcctgcactt attgcgctat gcggcctgat taggcaagtt aatcatcgtc
     4141 tcttgccgga aatccgcatt taaccacccc tccaagagca ccgcactaat tcgctttatt
     4201 tagttaatta gctaaattta caatttacat tatatctcat cattgacatc gtccccgcag
     4261 cggcacaacc gccggccaaa aggccgaaac cggggttgcc cagaagcctg caaggccaca
     4321 aacctgcagt tttccgggca tccctgaact aggagcaaat gaatgtttaa caagacgagt
     4381 tccccacaaa gcaaaggtgc caccctgctg tttctggccc tttcgttcgc gatggtcgcc
     4441 ggcacgaccg catcgctaaa agcacaggca gcccctgctg ctacgccagc tgcagcggcc
     4501 ccggcacctg ccggcgaaac tgcccccctg ggtgctgaag gcaacaaaga agccgccgcg
     4561 ttcgccgagg ccggctcgct caatatcttc gagagcaaag acccggcgct ctggattttg
     4621 atggtactct tcctggtggc cctcgcaatc ggtgccgaac gcctctgggt tctgctgcac
     4681 accaaatctg ccaatgcgga gctggtgcgc ctcgtgacgg agcgcttgag ccaaaacccc
     4741 gctacaggcg aagaattggc gaaagatgtc tcagaggcaa aattcggcgt cgagggccgt
     4801 atcgctgccg tcaccctcaa gggctggcag cacgacgaag acacgctgca atcttacgcg
     4861 aatgcagcaa cagtggcaga gcgccgctac ctgagtgcac gccttccggt actttcaaca
     4921 ctcggtaaca atgcgccgtt catcggtctg ctcggcaccg ttctcggtat catgaaagcc
     4981 tttcgcgacc tagctggtgc ggctgatgcc ggaccgcagg tagtgatgaa aggcatttcc
     5041 gaagccctgg tggcgactgc aatgggtctc ggtgtggcaa ttccatgcgt aatgctcttc
     5101 aactacttct cgtctgcgat taagcgcaag ctttccaact ctgaagaagt cactaacatt
     5161 atcgtcgcgc tgcgccgcgc cacacgctcg ggcaacaagg tagtctaaga tggcagccaa
     5221 agtaggcaac gactcgggcg acgaagtcat tggcgacatc aacgtcgtcc cgctcgtcga
     5281 catcatgctc gtgcttgtga tcatcctgat ggtcaccgcc gagttcacga aataccgcac
     5341 tgtccctatc cagctgccaa aggtcaatgc cgtcgcgatg aaacgcgaac cgcacaaggt
     5401 tgcgatcacg attaagccca acggcaaggt atacctcggc gataaagaga taaaagacct
     5461 cgcagaactt ggcccccgac tcgtttcagc caagaaggct cagcctgacg tggccgtgat
     5521 actgcgcatg gaaaaggaca ccaagtacag cgacatgctc aaggtactcg atgacgtgaa
     5581 actctcaggt atcagcaagg tcggcctcgc cgtcgattcg gcgaaaaaag gtaagtagta
     5641 tgacggcagc gattacccca cccccggctc ctccccttca caaggggagg ggtgccgaag
     5701 gcggggtggg gtctatatct aaacgttcgt cacgcagcgg cgtagtaagt tatgacggta
     5761 tccgtctttc tgacgggcgc ttcgtctacc ctggcctctc gaacacgttc gagaactacg
     5821 agacgattcc ctggatcagc cgcaacaagg cactctggtt agggggagct ctcctggttc
     5881 tgtcgattgc gatcactgta gtcaaaatcg actggagtcc tgatgactca gagctcgaca
     5941 aactcgcggt aaccgtcgac ttcggcgacg tcgtgcaacc cttcaagaag aaggcagcga
     6001 agccgcgcga agaggtcgac gaagtcttcg gcaacgagtt cataaaagat aaaactcccc
     6061 cggaccccaa ccaggaagac ccgcgcatcg ccagcgctgt gaacgcagcg gtgggtggcg
     6121 cgacgatgcc cgtcgatctt tcgccggaaa ttcggccgca gtatactgcc gccgcgcgca
     6181 atgctggcat cgaaggtgct gtcacgctcg aaatcgtgat ctctgaagac ggcaaagtcc
     6241 tgcgcgcgaa gccagttggt aagaagctgg gcctgggtct cgacgaggct gctgccgcga
     6301 cctacaaagc caaacgcttt aagccctccg ttggaccggc ggggaaaccc atcgtggtga
     6361 aatactacga gcgcgtgaaa ttcatgctca attgatatga aatcccagaa cgataacgaa
     6421 cacccggggg cctaaaaccc ccgtttttcg gagc
//
LOCUS       DAOQJF010000189         6416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6785_length_6416_cov_4.068228, whole genome shotgun sequence.
ACCESSION   DAOQJF010000189 DAOQJF010000000
VERSION     DAOQJF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6785_length_6416_cov_4.068228"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLF85_10535"
     CDS             <1..853
                     /locus_tag="PLF85_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE20148.1"
                     /translation="LVTAWLADLKSKGVTFHFRHELSDIDRTPDGRYELAFGGGTRSI
                     ADMVVLALGGASWLPEGEVLKWPNLLGKLGIQVSPFAATNCGYAVEWKKEFLAEAKRA
                     PLKNVTLLTAKGSKKGDILITEYGVEGTPVYACGYTGLCHIDLKPDLDALQVLEKLRR
                     VQENLSPLRRAKRVLALSEAAIALLYHHSPADAVATNEGLAKLIKAFPLELLAPRPLT
                     EAISSSGGVKLDELTPDFELRGLPGVFAVGEMLDWSAPTGGFLIQACVSQAAVAGRAI
                     AQRLAAR"
     gene            910..1170
                     /locus_tag="PLF85_10540"
     CDS             910..1170
                     /locus_tag="PLF85_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20149.1"
                     /translation="MSNLETCTCGNTLQNASLYARGVYQRRDRVLLSLGATRRPIKVE
                     IRCVRCEHVFTVTDDYSVRDRACARDWLTDADLAACQAKTAA"
     gene            1197..1667
                     /locus_tag="PLF85_10545"
     CDS             1197..1667
                     /locus_tag="PLF85_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20150.1"
                     /translation="MSESERARLRSEARSYLAFFSVFGGAFGLASVFAAWPFEKIESF
                     RWMLSGVLLVFFFIFLGVYLFQVKGVLRSLAGGKSVMYTGRISRKNLMQVDVGLVYEI
                     YIGERKFEIDVSLYDNVKIGDRIAVREWLKSGEYLDHTTDERRIAELEARALAE"
     gene            1709..2680
                     /gene="pfkA"
                     /locus_tag="PLF85_10550"
     CDS             1709..2680
                     /gene="pfkA"
                     /locus_tag="PLF85_10550"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNE20151.1"
                     /translation="MVERIAVITSGGDVPGLNACIRAIVHTAAHHKIEVMGVRHGYDG
                     LISGELVPLNVDSVHDIMQRGGTILHSARSKRFETGEGRTAAAQTLAKHGITNMIVIG
                     GDGSLTGAGLFAKEHGLHIVGIPKTIDNDVTGTDICIGYDTAVNTAMEALDRIRDTAD
                     SHERMFVIEVMGRDSGYIAYAAGIAGAADGILIPETTTDWNQLTQVLHERHEGRTGSL
                     LIVVAEGDESGGAEKLGKKLAQEFPNEKVGVCVLGHTQRGGSPSAADRILASRFGVAA
                     VDALAAGKTNIMVGIQGDQIVFTPLNEIRRKHLELTAERQRLLQMLL"
     gene            complement(2675..>3283)
                     /locus_tag="PLF85_10555"
     CDS             complement(2675..>3283)
                     /locus_tag="PLF85_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20152.1"
                     /translation="PKETPLFDHTTTSIEGARRRTADLGAIGVTYQHAPVFMSPQNAL
                     DAAGVMLISGERSRYEKYREHLEPMTGKLVYLGDDIGRAAAMKLGGNLMLMAITAGLT
                     DMARFLKGADVPREAINQLFESFNPGMSAQPRAKRMFSGEYENPSWELKMARKDARLI
                     LEAADQNNLPLKFLPAIATEMDRFIAAGDGERDWTVLGRELL"
     assembly_gap    3284..3383
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3384..3678)
                     /locus_tag="PLF85_10560"
     CDS             complement(<3384..3678)
                     /locus_tag="PLF85_10560"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE20153.1"
                     /translation="MLAFLGTGLLGSGFVRAALKRGERVAVYNRTLEKAAALGKEGAQ
                     VFPLAADAVKNALAVHLVLSDDAAVEAVLKDILPALPKETPLFDHTTTSIEGAR"
     gene            3768..5012
                     /locus_tag="PLF85_10565"
     CDS             3768..5012
                     /locus_tag="PLF85_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNE20154.1"
                     /translation="MLQEIHPFVLSLILGLLIGVEREHNERGAQKTLGVRTFAFVALA
                     GSLAAWLKNPVLAAVLGIAIFALILLGYWRSTGGHKKNADPGLTTEIAAIVTFCTGYV
                     LIHSVKLGILIGLTTLALLISRGWMHRFVREKLRKQELTAAAVLIILFFGVAPFLPDA
                     TIDPLGIVNPRSLLMLFALIALLQFAGYAANRLFGARSGLALSGFLGGFVSSTAVFVA
                     LPETRRSNPREEWLVTSHALLAVSATLIQLGLVVTLAEPALAASLALPLLVMVLTSGA
                     IALLLARGNASKGGRTTLRDPVDFRSVLKLALVLGGLIAIVNLVHRAFDDRGLWLVAG
                     ISGLFELQGVSLAIALDLKRGAVSLQASSVAVMVAVAASFVSKLVIVAFAHRDRFGLR
                     LAAALLVVMATGITMAMVLPLI"
     gene            complement(5201..6187)
                     /locus_tag="PLF85_10570"
     CDS             complement(5201..6187)
                     /locus_tag="PLF85_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20155.1"
                     /translation="MLIMRPLWKYAAFATALSLIAPLGATLNFNIPLAADLTEAQEFD
                     AKYTKLAQSFSRYYGEAMAFSSHLSAPIARDNLGKFPSLYVGIGLGASFGNVNGMKSE
                     VDSSISGNVPSYLAAPNISFNFGMGINRKWDFRFSFFPNVPIALDSASLGSNTSAEIK
                     FGTYRARAGYHILEGGFMKPGLTLAGFASYTTGALNLTQRSQNIESGDVKLSNATSTF
                     ATNWQYLGVGPEVRVWYDLMFFHPFIGYSLGLQMGQYTTGLNVEGTIEVNATSYGTGS
                     LVISERQAARLISHRLMFGFEIALFVLDIGVEAQVDLTSGLVGVAAGTAFRF"
BASE COUNT         1352 a         1805 c         1811 g         1348 t
ORIGIN      
        1 ccttgtgacc gcctggctcg ccgacctgaa gtctaagggc gtcacttttc actttcgcca
       61 tgaactgagc gatatagaca ggactcccga cggccgctat gagctcgcct ttggtggcgg
      121 aaccagatcc atcgccgata tggtggtact ggcgcttggc ggtgcaagct ggttacccga
      181 gggcgaagtg ctgaaatggc ctaacctgct cggcaagttg ggtatacagg ttagtccatt
      241 cgccgcaacg aactgcggtt atgcagtgga gtggaaaaag gaattcctcg ccgaggccaa
      301 gagggcaccc ctgaaaaatg tgacgctgct tactgccaaa ggaagcaaga aaggtgatat
      361 cctgatcacc gaatacggtg tcgaaggcac gccggtctat gcgtgtggct acaccggtct
      421 ctgccacatc gatctcaaac ccgacctgga cgcgttgcag gtgctggaaa aattgcgccg
      481 agtccaagag aacctctctc cgctgcggcg cgcgaagcgc gtgcttgctc tcagcgaagc
      541 cgcgatagcg ctgctctacc atcactcccc cgccgatgcg gtagcgacaa acgaaggcct
      601 cgcaaaactg atcaaggcat ttccgctcga gctactggca ccgcgaccgc ttacagaagc
      661 aatctcttct tcgggtgggg tgaagctcga tgaactgaca cccgattttg aactgcgcgg
      721 gttacccggc gtcttcgccg tcggcgaaat gctcgactgg agcgccccca ctggcggctt
      781 tctgattcag gcgtgcgtgt cgcaggccgc tgtcgcaggc cgcgccatcg cgcaaaggct
      841 ggcagcacgg tgacaccgat acccctggga atattggccg cgccggacgc gccggattat
      901 tatttctatg tgagcaatct tgaaacttgt acttgcggca acacgctaca gaatgcttcg
      961 ctctatgccc ggggcgtata ccagcgccgt gatcgcgttt tgctctcgct gggcgcaacc
     1021 cgccggccca tcaaagtgga aatccgctgc gtgcgctgtg agcatgtctt taccgttacc
     1081 gatgattaca gcgtgcgcga ccgcgcctgc gcacgcgact ggctgaccga tgctgacctg
     1141 gctgcctgcc aggcaaagac cgcggcatga acggttgccg gcgactcatc atacccatga
     1201 gcgaaagcga gcgcgcgcgg ctgcgcagcg aagcccgctc ttacctggct tttttttccg
     1261 tttttggcgg agcattcgga cttgcctcgg tctttgctgc ctggcctttt gaaaaaattg
     1321 aatcctttcg ctggatgctt tcgggagtgc tactggtttt tttcttcatc tttctggggg
     1381 tctacctttt ccaggttaag ggagtgctgc gcagccttgc cggaggtaaa tcagtcatgt
     1441 acaccggaag aatcagccgc aaaaatctca tgcaggttga tgtaggcctg gtgtacgaaa
     1501 tttatatcgg cgaacgcaag ttcgaaatcg acgtatctct ttacgacaat gtgaaaattg
     1561 gcgaccgtat cgcggtacgc gaatggctca aatcgggtga atacctcgat cataccaccg
     1621 acgaacgccg catcgccgaa ctagaagccc gcgctctcgc cgaataacaa atttctcgac
     1681 gcggcctgta gaaaattaac tttccatcat ggttgaaaga atcgcagtca tcacctcggg
     1741 gggtgacgtg cccgggctca acgcctgcat acgcgccatt gtgcacaccg cggcacacca
     1801 caaaatcgag gtcatgggtg tgcggcatgg ctatgacgga ttaatctcgg gggaactcgt
     1861 gccgctgaat gtcgattcgg tgcacgacat catgcagcgc ggcggcacga ttctgcactc
     1921 ggcgcgcagc aaacgttttg aaaccggcga aggccgcacg gcggctgcgc aaaccctcgc
     1981 gaaacacggc attacgaata tgattgttat cggcggcgat ggttcgctga ccggcgcagg
     2041 tcttttcgcg aaagagcatg gcctgcacat cgtcggtatt cctaaaacga tcgataacga
     2101 cgttacgggc acagatatct gcattggcta cgacacggcg gtcaataccg cgatggaagc
     2161 actcgaccgt attcgcgaca ccgccgattc acacgagcgc atgttcgtga tcgaggtgat
     2221 gggccgtgat tcgggttata ttgcttacgc cgcaggtatt gccggtgccg cagatggtat
     2281 attgattccc gagaccacga cagactggaa ccagctgacg caggtgctgc acgaacgcca
     2341 cgaggggcgc accggatcac tgctgatcgt cgtcgccgaa ggcgatgaat cgggtggcgc
     2401 agaaaaactg ggcaaaaagc tcgcgcagga gtttcccaac gagaaagttg gcgtctgtgt
     2461 gctggggcat acgcagcgcg gcgggtcacc gagtgccgca gatcgcattc tcgcctcgcg
     2521 ctttggtgtc gcggcggtcg atgcacttgc tgccggcaag acgaatatca tggtcggcat
     2581 ccagggcgac cagattgtgt tcacgcctct gaacgaaatt cggcgcaagc atctcgagct
     2641 cacagcagag cgccagcgcc tgctgcagat gctgctataa caattcgcgg ccgagcaccg
     2701 tccagtcgcg ctcgccgtcg cctgctgcaa tgaagcggtc catctcggtc gctatcgcgg
     2761 gcaaaaactt cagcggcagg ttattctgat ccgccgcttc gagaatcagc cgcgcgtctt
     2821 tgcgcgccat tttgagttcc cacgaagggt tttcgtattc gccgctgaac attcgcttcg
     2881 cgcgcggctg cgcactcata ccaggattaa aactttcaaa cagttgattg atcgcctcgc
     2941 gaggtacatc cgcccccttg agaaaacgcg ccatgtcggt aaggcccgca gtaatcgcca
     3001 tgagcatcag gttgccgccg agcttcatcg ccgcggccct gccaatgtca tcgccaagat
     3061 aaaccagctt acccgtcatc ggctcgaggt gctcgcggta tttttcatag cgcgacctct
     3121 cgcctgagat gagcataacc ccggcagcat cgagggcgtt ctggggcgac atgaaaacag
     3181 gtgcgtgctg gtaggtaaca ccgattgcgc ccaggtcagc cgtgcgccgg cgagcacctt
     3241 caatcgacgt cgtcgtgtgg tcaaaaagcg gagtttcttt tggnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnggcgagc accttcaatc gacgtcgtcg tgtggtcaaa
     3421 aagcggagtt tcttttggca gcgccggcaa aatatctttg agaacagctt cgactgctgc
     3481 gtcgtcagac aacacgaggt gcacggccaa agcattcttc accgcatcgg ctgcgagggg
     3541 aaaaacctgc gcgccttctt ttccgagtgc cgccgctttc tcgagcgtgc ggttgtaaac
     3601 cgccacgcgt tcgccacgct tgagcgccgc gcggacaaaa cccgaaccga gaagccctgt
     3661 acccaaaaat gccagcatgc gccgtgacag ggcggcaaaa tcgggcggca agcctttgca
     3721 aactgcatcg gctttacagc ctgccgcctg aggcaaagtt aatcgcggtg ctgcaagaga
     3781 tccatccgtt tgttttgtcg ctgattctgg ggcttctgat cggcgtggag cgtgaacaca
     3841 atgaacgcgg cgcgcagaag actctcggcg tcagaacatt tgctttcgtt gcactcgccg
     3901 gctccctcgc ggcgtggctg aagaacccgg tgctcgcagc agttctcggt atagcaatct
     3961 tcgcgctgat tctgctcggt tactggcgtt caaccggcgg gcacaagaag aatgcagacc
     4021 cgggactaac gacagaaatc gccgcgatcg ttactttctg cactggctat gtgctgatcc
     4081 acagtgtgaa actcggtatt ctgatcggtc tcaccaccct tgccttgttg atttcacgcg
     4141 gctggatgca ccggtttgtg cgcgaaaagc tgcgcaagca ggaactcacc gcagcggcgg
     4201 tactgatcat tcttttcttc ggcgttgccc cgtttctgcc tgacgccaca attgacccat
     4261 tgggcatcgt caacccgcgc agcctgctga tgctctttgc gctgattgcc ctgctgcaat
     4321 ttgcgggtta tgcggcgaac cgcctgttcg gtgcgcgttc tggcctcgct ctctcgggtt
     4381 tcctgggtgg ctttgtttcc agcacggccg tttttgtcgc gctgccagag acacgccggt
     4441 ctaacccgcg cgaagaatgg ctggtgactt cgcacgcact gcttgcggtc agcgcgacgc
     4501 tgatacagct tggtctcgtg gtcacactcg ccgaaccagc tcttgctgca tcgctcgcct
     4561 tacccctctt ggtgatggtt ctcacgagcg gagcgattgc acttctgctg gcccgcggca
     4621 acgccagcaa gggcggcagg acgacgctac gcgaccccgt ggattttcgt tctgtgctga
     4681 aactggcgct ggtactgggc ggcctgatcg cgattgtgaa cctggtgcac cgcgctttcg
     4741 acgacagggg tctttggctc gtcgccggta tttcaggtct gtttgaactc cagggtgtga
     4801 gcctggcgat tgcactcgac ctcaaacgag gagcagtctc actgcaggca agctcggtcg
     4861 cagttatggt tgcagtcgct gcatcctttg tctctaagct ggtaattgtg gcttttgcgc
     4921 acagggaccg attcggctta cgcctggcgg cggcattgct ggtggtgatg gcaaccggca
     4981 tcaccatggc aatggtactg ccgctgatct agggaatctc tgaaaaaggc gcgaaccgcg
     5041 ctgaacaggc gcggacgttg cgcgagaaca ggccattcat ggccgttttg ccgtcggcca
     5101 aagcgccgcc ggccacgctg cgatgcacgc cgctttggcg agcgatttcg tcaggaaatc
     5161 gcggcgttct tgcgcaacgg gcgacttttt cagagtattc ctagaatcta aaagctgttc
     5221 cggcggcgac gccgacaagg ccgctcgtca ggtcgacctg cgcttcgacg ccaatgtcga
     5281 gtacaaacaa ggcaatttca aaaccaaaca tcagccggtg cgagatgagt cttgcagcct
     5341 gccgttcaga gatgacgagc gaacctgtac cataagaggt tgcattcacc tcgattgtgc
     5401 cttcaacgtt caggccggtc gtgtactgcc ccatctgcag accaagggag taaccgataa
     5461 aaggatggaa gaacatcagg tcgtaccaga cccgcacctc tggacctact cccaggtact
     5521 gccagtttgt cgcaaaggtt gaagtggcat tagagagctt cacgtcacct gattcaatat
     5581 tctgcgaacg ctgggtcagg ttcagcgctc ccgtggtgta tgaggcaaaa ccggcaagag
     5641 tcaggcccgg cttcataaag ccaccttcga gaatgtgata cccagcgcgg gcacggtacg
     5701 taccgaactt aatttcggcc gaagtgttgc tgccgagact cgccgaatcg agcgcaatgg
     5761 gaacattagg aaagaaagaa aagcggaaat cccacttgcg gttaatgccc ataccaaaat
     5821 tgaacgaaat gttgggcgca gccaggtaag aaggcacatt gccgctgatc gacgagtcaa
     5881 cttcgctctt cataccattg acgttaccga atgaagctcc caacccgata ccaacataga
     5941 gcgacggaaa tttgccgagg ttatcgcgtg caatcggcgc cgacaggtgc gaagaaaaag
     6001 ccatcgcttc gccatagtat ctagaaaaag attgtgcaag cttcgtgtac ttggcgtcga
     6061 attcctgcgc ttcggtgagg tcggcagcca gcggaatatt aaaattgagc gtcgcgccca
     6121 gtggagcaat caacgagagc gcagtggcaa aagctgcgta tttccatagg gggcgcatga
     6181 tcagcatata ggacggacct gtgggtatat tatcgacagc aaagcggccc gactcaaggc
     6241 ggcgcgcaac ccggacaaac tggatcccct gcttccaaac ccggcctcag gattcccgca
     6301 tgaggaatgc ttgacgtcac aaaactgacc gatcgttcgg tcggtactcc ccgatggaga
     6361 cagtatggaa ctaaaaccga aggatatcct gagcagtgac gagatggccg cactac
//
LOCUS       DAOQJF010000190         6349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6894_length_6349_cov_5.564665, whole genome shotgun sequence.
ACCESSION   DAOQJF010000190 DAOQJF010000000
VERSION     DAOQJF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_6349_cov_5.564665"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLF85_10575"
     CDS             <1..717
                     /locus_tag="PLF85_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20156.1"
                     /translation="REVYYLAGFDIRDPFSGKKTTLAAEDNIYTARIRGLETGTSVIS
                     LGIIYADGTRAAPRHFYFDSNDPRARKKFAFTDIEYEKVVPIRERNAFKVGGRAELIS
                     SKFDRGILEIRLNYDTTDACRNAAVRGCKPKQIKVKGFVWEIASAERVPQGEINFSGP
                     VEFIYLQKPGQYFLNAKTLFKGPEREQFAYDSRSVDVPDVRKEFRKQLYTALATALAF
                     LLLIALWQRRRIAFYAGSWS"
     gene            717..2450
                     /locus_tag="PLF85_10580"
     CDS             717..2450
                     /locus_tag="PLF85_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20157.1"
                     /translation="MRLLYPVVLAASLLLRPLPAAKTGEWPISGLKLVQTQWSSWVTR
                     FFATDDEQNLIYGRLIDQKPELQKVMRFRRLPDLVKHFPMADGDLIVSVYNPEKYMHF
                     VAFDFTGAVKYSNEFPLDYNVYDVDARVNIEAGNPSCLLYAFDKRNYYIKYWSERRTQ
                     NIMASRDPIDLMFLQWADGGIHYVSAASNGHTWTFWKNGEYRAYPLPFPIKHAKLYQF
                     RNAMHLIGIDLEGGLWQFDISSGELRRNLLARDARLVLAEKVIPFNFNRELNIIVTSP
                     HVGTAYRLTFDDFPRPRRKPKLEERRLFWPGNVYPIIDRSNQLDFLLETEIRHIFFES
                     WNAPTVLISDIDWRLDVKRNPPEMMVAWSIPKGAQYAYRYLLDQNPDSEPLSDTKLIP
                     SNTLQFAARKEGAYVLHMQVRNLRTGSYSRVYHIPLLWQYAPPEPEVILANQVAPRMV
                     SPGRVDFLLNNPHPGDYYAVLDGKPDTVPNRPISVASGRLSFNARAKGSYYLHLANRD
                     PRSRVLSPVAHYLFFVSPFDAEEDPALAENLRRIDEIRRIKIKIQNAKGDPAATQLWI
                     NRLQEIEAQLK"
     gene            2447..3628
                     /locus_tag="PLF85_10585"
     CDS             2447..3628
                     /locus_tag="PLF85_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNE20158.1"
                     /translation="MRLPPDPAPVEAEILQFFADRNFERSRRFSGNAYDPAILRDYLA
                     TAGNPQFGFRAIHVAGTVGKGSATTMLARALSAMGFRTGAYLSPHFVSLTERFVIGDE
                     PISPAALEIAWQKLRANGGLESLSFFDAMTALGFLYFAEAGCDFAVIETGLGGRLDST
                     NNLRAEAAVLTTIGMDHMNILGDTLTAIATEKAGIIHPGQKVYSLPQGGEALRVIEAR
                     CREVGAALTVIQPTGEDFTSRNGDFILQLLGLHFAPREAERSLCRSAIEAPIFGRWSQ
                     LTAQPRVFFDGAHNAAGIGALCELVNRQPESECNIFLNTMRERDLGAFGQMLLQQINK
                     KVNLYLFPMPGDNYYPEAPQPLSAADDTAIRQLVTEPQKLHLFTGSMGLYAELRRRFA
                     L"
     gene            3625..4941
                     /locus_tag="PLF85_10590"
     CDS             3625..4941
                     /locus_tag="PLF85_10590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE20159.1"
                     /translation="MKYLLLGLFFCASQLSALPELDAAIAGKDQGKAIEIYATRDLNE
                     TELDQLLQFIAGEKEKYRKKLSYAIRDRKATDAVAGRHLHNELLRLIKDNLVEDDTDY
                     TEALLLASGINKNPDLIYSIAPFLVHPRLELRQEANRAVALKKDERIYPLIGQLLAGE
                     NVIDKVYAMETLLALKDERAVPLLLLQLSNQSKNVRYFALKTLEAIGSDKAQYGVINL
                     AQGDADEEVRLKAVEILRQFKTGPVFYALQKLIADPQLIIRTRALESALAQLDKKYAN
                     SISEQLARETDMTQKHALLRGLLTLGSGGGMNGILTLLKKESDADLLLWSAFACNRFS
                     DSRCADLFAQLLGSRSEQSIVLEAVVGLAQHRQRKHLPVLFTLAQDATKPQVLRSAAL
                     TAIQQYDNEAAIAPLFNLYESEQDQLIRVQARTLMTDLMKKKLPKL"
     gene            4938..5402
                     /locus_tag="PLF85_10595"
     CDS             4938..5402
                     /locus_tag="PLF85_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20160.1"
                     /translation="MNERCRYTLLIEYAGDDELIEAIGQIEKQLPDYDYASRPYPLTT
                     GNGFFKFLAFKDLKGVLDFPKFARKALVLQNRLRGVRLTPGYVSMVGAATAGYSHSPG
                     GLLVEKELWLKPQLVLSGKLLASHSLTDEILKDKRSVIYLNDVWQLVKGATR"
     gene            complement(5411..5794)
                     /gene="crcB"
                     /locus_tag="PLF85_10600"
     CDS             complement(5411..5794)
                     /gene="crcB"
                     /locus_tag="PLF85_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNE20161.1"
                     /translation="MLKELLLVAVGGLTGSVFRFLVFKFGSHFSPAWFPFSTFIVNLA
                     GSFLIGVLYALVERSAGLAGTLRPLLMAGFCGGFTTFSAFSLENYQLLKEGQYLAVFA
                     YATASVILGLAAVILGVKVFTLLAE"
     gene            complement(5840..>6349)
                     /locus_tag="PLF85_10605"
     CDS             complement(5840..>6349)
                     /locus_tag="PLF85_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNE20162.1"
                     /translation="AVISLGGMVIADSIRIGGDEFDEAIIKHLRLHHNLIIGERTAEE
                     IKLAIGNAMPEKKTETFELKGRDAGTGLPRTVVIDSAEVRKALKEPIDAIVEATKIAL
                     EKTPPELAADIVERGIVMTGGGCLLKGLDKHISKETGVPVIRAENPLNCVVEGTGKYL
                     EELKYLKGR"
BASE COUNT         1428 a         1881 c         1743 g         1297 t
ORIGIN      
        1 cgtgaagtgt actacctcgc gggctttgac atccgcgacc cctttagcgg taaaaagacc
       61 accctcgccg cagaagataa catctacacc gcgcgtatcc gcgggcttga gaccgggacc
      121 tccgtgatct cgctgggtat tatttacgcc gacggtacac gcgccgcgcc gcgccatttc
      181 tactttgact ccaacgaccc ccgggcccgt aagaagtttg ccttcaccga tattgagtat
      241 gaaaaggttg tgcccattcg cgagcgtaac gcgttcaaag tgggcgggcg tgcagagctg
      301 atcagttcaa aattcgaccg gggtatcctg gaaatccgcc tgaactatga caccaccgat
      361 gcctgccgca acgccgccgt gcgcggctgc aaacccaaac agatcaaggt caaaggtttc
      421 gtctgggaga tcgcgagcgc cgaacgcgtg ccgcagggtg aaattaattt cagcggcccg
      481 gtcgagttca tctacctgca gaaacctggc cagtacttcc tgaacgccaa gacgctgttc
      541 aaaggtccgg agcgcgagca gtttgcgtac gattcacgca gcgtcgatgt gccagacgtg
      601 cgcaaggaat ttagaaaaca gctctacacc gcgctcgcca cggcgctcgc ctttctgctg
      661 ctgatcgcgc tgtggcagcg gcggcgtatc gccttttacg cggggtcgtg gagttgatgc
      721 ggctgcttta ccctgtggtt ttggcagctt ctctgctgtt acgcccgcta ccagctgcga
      781 agaccggcga gtggccgatc agtggcctga agctcgtgca gacacagtgg tcttcatggg
      841 ttacgcggtt ctttgccacc gacgacgaac agaacctcat ctacggcagg ctgatcgacc
      901 agaaacctga gctgcagaaa gtgatgcgct ttcgccgcct gcccgatctc gtgaaacatt
      961 tccccatggc cgacggtgac ctgattgtca gcgtctataa tcccgagaaa tacatgcact
     1021 tcgtggcatt tgacttcact ggcgcggtga agtactcgaa cgaatttcca ctcgactaca
     1081 atgtctacga tgtcgatgcg cgggtgaata tcgaggcagg taacccgtcc tgcctgctgt
     1141 acgcattcga taaacgtaac tactacatca agtactggtc tgagcggcgc acgcagaaca
     1201 tcatggcaag ccgcgacccg atcgacctga tgttcttgca gtgggccgac ggcggcattc
     1261 actatgtcag cgccgcgtca aacgggcata cctggacttt ctggaagaac ggcgagtacc
     1321 gcgcataccc cctgcctttt cccatcaagc atgcgaagct ctaccagttt cgtaacgcga
     1381 tgcaccttat cggtatcgat ctcgaagggg ggctgtggca atttgacatc tcttcgggcg
     1441 agctgcggcg taacctgctc gcacgggatg cccggctggt actcgccgaa aaggtcatac
     1501 cgttcaactt caaccgcgaa ctgaacatca tcgtcacgag cccgcacgtc ggcacagcct
     1561 accgccttac cttcgacgat tttccccggc cgcgacgcaa accaaaactc gaggagcgcc
     1621 ggctgttctg gcccggcaac gtgtacccca tcatcgaccg cagcaaccag ctcgactttt
     1681 tgcttgaaac agaaattcgc cacatcttct tcgaatcctg gaatgcgccg acggttctta
     1741 tctctgatat cgactggcgg ctcgacgtga agcgcaaccc acccgagatg atggtggcct
     1801 ggtccatacc caaaggtgca cagtacgcct accgctacct gctcgaccag aaccccgaca
     1861 gcgaaccgct ctctgacacg aagctgatac cgtcgaatac actgcagttc gccgcgcgca
     1921 aagagggtgc ttatgtgctg cacatgcagg tacgcaacct gcgtaccggg tcctattcgc
     1981 gcgtgtacca tattccgttg ctgtggcagt acgcgccgcc cgaacccgaa gtcatcctgg
     2041 cgaaccaggt ggccccacgc atggtatcac cagggcgcgt cgactttttg ctgaataacc
     2101 cgcacccggg tgattattac gccgtgctcg acggcaaacc cgacacggtg ccgaatcgcc
     2161 ccatttcggt ggcgtcgggc cgcctgtcgt tcaatgcgcg cgccaagggc agttattacc
     2221 tgcacctggc aaaccgtgac ccgcgcagcc gggtcttgag cccggttgcg cactacctgt
     2281 ttttcgtttc acctttcgac gcagaagaag atcccgcgct ggcagaaaac ctgcgccgca
     2341 tcgacgagat ccgccgcatc aagataaaga tacaaaatgc aaaaggtgat cctgcagcaa
     2401 cgcaactctg gataaaccgg ctgcaggaaa ttgaggcgca gctgaagtga gacttccgcc
     2461 tgacccggca ccagttgaag cagagatcct gcagttcttt gccgaccgca acttcgagcg
     2521 cagccggcgt ttttcgggca acgcgtatga tcccgccata ctgcgcgact accttgcgac
     2581 tgcaggcaat ccacagtttg gtttccgcgc catacacgtc gcgggtaccg tcggtaaggg
     2641 cagcgccacg accatgctgg cgcgcgcgct gtccgcaatg ggatttcgga cgggggcgta
     2701 cctttcgccg catttcgtga gcctgaccga acgttttgtg atcggtgatg aaccgatttc
     2761 ccctgcggcg ctcgaaattg cctggcagaa gctgcgcgcc aacggagggc tcgaatcact
     2821 ctcgttcttc gatgcgatga ccgcgctggg cttcttgtat tttgctgagg caggctgcga
     2881 tttcgcggtc atcgaaaccg ggctgggtgg caggctcgat tcgacgaaca acctgagggc
     2941 cgaagccgca gtactgacca ccatcggcat ggaccacatg aacattttgg gcgacacgct
     3001 cacggcaatc gcgaccgaaa aggcaggcat catccacccg ggccagaaag tctatagcct
     3061 gccgcaggga ggcgaggcgc tgcgcgtcat cgaagcgcgc tgccgcgagg tcggcgcggc
     3121 gctgacggtt attcaaccta cgggtgaaga ttttaccagc cgcaacggcg actttattct
     3181 gcagctgttg gggttacact ttgccccccg cgaggcagag agatcgctct gccgcagcgc
     3241 catcgaggcc cccatctttg gccgctggag ccagctaaca gcccagccac gggtcttttt
     3301 cgacggcgcc cacaacgcgg cgggtatcgg ggcgctctgc gagttagtta atcgacaacc
     3361 tgaatccgaa tgcaatattt ttctcaacac catgcgcgaa cgcgatctcg gcgcctttgg
     3421 acagatgctg ctgcagcaaa tcaacaagaa ggtgaacctg taccttttcc cgatgccggg
     3481 ggataactat tatccagagg ccccgcagcc gctgtccgca gcggatgata cggccatccg
     3541 gcaactggtc acagaaccgc agaagctgca cctgtttacc ggcagcatgg ggctttatgc
     3601 cgaactacgg cggaggttcg cgctgtgaaa tacctgctgc tgggactttt tttctgcgcc
     3661 tcacaacttt cggccctgcc cgaactcgac gcagcgattg caggtaaaga ccagggtaag
     3721 gcaatcgaaa tctatgcaac ccgcgatttg aacgaaacgg agctcgacca gctgctgcag
     3781 tttatcgccg gcgaaaaaga aaaataccgc aagaaactca gctacgccat tcgcgaccgc
     3841 aaggcaacag atgccgtagc aggccggcac ctgcacaacg aacttttacg gctcatcaag
     3901 gacaacctgg ttgaggacga caccgactac acagaagcat tgctgctggc ttcaggcata
     3961 aataagaacc ctgaccttat ctactccatc gccccttttc tcgtgcaccc ccggctcgag
     4021 ctgcggcagg aagcgaaccg ggcggtggcg ctgaaaaaag acgagcggat ctatcccctg
     4081 attgggcagc tgctcgccgg tgaaaatgtc atcgataaag tttacgcgat ggaaacgctg
     4141 cttgcgctca aggacgaacg cgcagttcct ttgctgctgc tgcagctctc taaccagagc
     4201 aaaaatgtca ggtactttgc cctcaagaca ctcgaggcaa tcggctcaga caaggcgcag
     4261 tacggcgtca ttaacctggc acagggcgac gccgacgaag aagtcaggct gaaagcagtc
     4321 gagattttgc ggcagtttaa gactggtccg gtattctatg cgctgcaaaa gctgattgca
     4381 gacccacagc tgatcattcg cacgcgcgcg cttgaatcgg cgctggcgca gctcgacaag
     4441 aaatatgcca actccatctc ggaacagctg gcgcgtgaga ctgacatgac gcagaagcat
     4501 gcgctgctac gcggcttact cacgctcggc tcaggcggag gtatgaacgg tattcttact
     4561 ttgctgaaaa aagaatctga tgccgatctg ctgctttggt cggcatttgc ctgtaaccgt
     4621 ttttctgaca gccgctgcgc cgacctgttt gcccagctac tgggcagtcg cagcgaacaa
     4681 tccattgtac tcgaagcagt ggtcggcctc gcgcaacaca ggcagcgcaa gcacctgcca
     4741 gtacttttta ccctcgccca ggatgcgacg aaaccgcagg tattgcgctc ggcggccctc
     4801 accgccatac agcaatacga taacgaggcg gccatagccc cgctttttaa cctctatgaa
     4861 agcgaacagg accagctgat tcgggtgcag gcgcgcaccc tgatgactga cctgatgaag
     4921 aagaaattac ctaagctatg aacgaacgct gccggtatac gctgctcatc gagtacgcag
     4981 gtgacgacga gctgattgaa gcgattgggc agattgaaaa gcagctgccc gattacgatt
     5041 acgcgtcgcg gccttacccg ctcacaaccg gcaatggctt tttcaagttt ctcgcattca
     5101 aagacctgaa gggagtcctg gattttccga aatttgcccg caaggcgctg gtcttgcaga
     5161 accgcctgcg gggagtacgc ctgacgcccg gctatgtttc gatggtgggg gctgctacgg
     5221 cgggctacag ccattcaccc ggtggcctgc tggtagagaa agaactctgg ctgaaaccgc
     5281 agctggtact ctcgggcaag ctgctcgcca gccacagcct gaccgacgaa attctaaaag
     5341 acaaacgctc tgttatctat ctcaacgatg tctggcaact ggtcaagggc gcaacaaggt
     5401 agcgcagcaa ttattcagca agcagcgtaa aaactttcac gccgagaatc actgccgcca
     5461 accccaggat tacactggcg gtcgcgtaag cgaacactgc gagatactgc ccttctttga
     5521 gtagctggta gttctccagt gagaatgccg aaaaggtggt gaatcccccg cagaaaccag
     5581 ccatcaaaag cgggcgcagt gtacctgcaa gaccggcgga gcgttcaacg agcgcgtata
     5641 aaactccgat gagaaaagaa ccggcgagat ttacgataaa agtgctgaac ggaaaccagg
     5701 caggcgaaaa atgactgcca aacttgaaga cgagaaatcg aaacacgctg cccgttaggc
     5761 caccgacggc aacgagcaac agttctttaa gcatgatgtt tttgcagtcg atggcgcaca
     5821 gggcgcatcg actgctccct tagcgacctt tcaggtactt gagttcttcg agatacttgc
     5881 ccgtaccttc gaccacgcag ttgagcgggt tttcggcacg gatcaccggc acgccggttt
     5941 ctttagaaat gtgcttatcg agtcccttga gcagacaacc accgccagtc atcacgatac
     6001 cgcgctcgac aatgtcggcg gcaagttcgg gcggagtttt ttcgagagcg atcttggtcg
     6061 cttcgacgat ggcgtcgatc ggttctttga gcgccttgcg cacttcggcg ctatcgatga
     6121 cgaccgtgcg cggcagaccg gtaccggcat cgcggccctt gagctcgaag gtttcggtct
     6181 tcttctcagg catcgcattg ccgatcgcga gtttgatttc ttctgccgta cgctcaccga
     6241 tgatcaggtt gtggtgcagg cgcaggtgct tgataatcgc ttcgtcgaat tcgtcgccgc
     6301 cgatacggat actgtcggca atcaccatac caccgagcga gataaccgc
//
LOCUS       DAOQJF010000191         6333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6925_length_6333_cov_3.947117, whole genome shotgun sequence.
ACCESSION   DAOQJF010000191 DAOQJF010000000
VERSION     DAOQJF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6925_length_6333_cov_3.947117"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..184
                     /locus_tag="PLF85_10610"
     tRNA            111..184
                     /locus_tag="PLF85_10610"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:145..147,aa:Asp,seq:gtc)
     gene            290..1051
                     /locus_tag="PLF85_10615"
     CDS             290..1051
                     /locus_tag="PLF85_10615"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20163.1"
                     /translation="MANELAKGLEYLTVLGPGKVIVLLSTMPGDKLAYLSRHIEHKKI
                     IQVLRATSVRQVSAIVKKTDVQALVPLMNVLDADESVDLLNRLELDTIVLFLTHLDPQ
                     RLAQIVRGVGVEKMLELLAATGTERALVLARELPSAHLVALVKKVPMRDLAPLAVSLR
                     VELLSNLLAADSAEHLAQLITTLGHRNLLDLIHFMGDKEITHLLQRYKGPKLEGLILL
                     LHGMRIKRPKPAKKKAVKKKPAKKKTAKGKNKRAA"
     gene            1090..2478
                     /locus_tag="PLF85_10620"
     CDS             1090..2478
                     /locus_tag="PLF85_10620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE20164.1"
                     /translation="MLFNSYVFLFLFFPVVLAGYYALPRPQWQLAFLLLMSLVFYAYW
                     SIPYLFLLIVVVVLDFWLAAAIHRSENTGRRRGLLILSLVLNLGLLFFFKYAGLVATT
                     INHFAPVLPAISLVLPIGLSFYTFQSLSYVIDVYRRHSSAHPSLLSYSTYVTMFPHQI
                     SGPLVRHNTIIPQLEDGATYRVQSDNLWRGAFFFVTGLAKKILIADFISRGINPIITN
                     MATASNSEAWLAMVGYTLQLYFDFSGYSDMATGLCLMLNIRLPQNFDSPYKSGSITEF
                     WQRWHMTLSSWLRDYLYISLGGNRGTKFATYRNLMLTMALGGLWHGATWMFALWGIYH
                     GLLLAIERATGIGRGITPNALRIALVFLLAALGWILFRAPDLSIATVWYQKVFLLNPT
                     LSWDLLSIPIKHRDRFGVAFLAGLVIVFAARNTWRIAFAPSYRNVLLLVLLFALCLPF
                     LGDESPFLYFQF"
     gene            2481..3482
                     /locus_tag="PLF85_10625"
     CDS             2481..3482
                     /locus_tag="PLF85_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20165.1"
                     /translation="MSSKKAISLFTLLSVAVIAAIAAFSYTVDPLCYYRCENVTAEKK
                     NLNGYHRNLQRILRYPDTELLVLGSSRGESLPLAWLAEEYSLKALNLSVGGADIHAKA
                     AFLQFALERLQLKKVIWIADYFELIDENMSDKVSLTPALRNLAAKSDTPALGSSLKRL
                     PTLIEHNTIEAGFALLRGKKVQWPPDRGENSGLDLPHCRQSVSDAKLTEEKLLKEVGM
                     IYDGYAGRILLPEQNPNSYKKMRELAQTLSARGISVIIVIPPYHPEFMRRLKAEQPQI
                     FSRHEVWAKKLGALAGPNAEIRDFFSMARFASAPALHWSDGVHFNCAAAAEMLSRKR"
     gene            3535..4671
                     /locus_tag="PLF85_10630"
     CDS             3535..4671
                     /locus_tag="PLF85_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE20166.1"
                     /translation="MIDYRAIRAQFAVREDLIWLNNCGVSVPPKSSAQAVTEYLEAFS
                     ASGIMQTAKPHGQVKRNIQSLFAKFLGGSPHEYAILNNTAEGMTFIAQSLPLQRGDKI
                     LLVEREYPSNVYPFLQLKRQGIEIGYVNPGLSNDEFLDNISKSLTTNVRVMSLSAVDW
                     LTGLRFDLNRIGRLLQSRGVAFILDAAQGAGHVEIDVKEAGIDAMAFSCWKWLLGPLG
                     SGGLYVADDFLKKLDIRVAGTSSVKNDDTYLPHREDYKESVERFMLSTAPYMNWVFLE
                     SSLQFIDTVGLETVQQRNLELAERLSGILRKAGYEVLRDRFGHDQTAIVCARRDGDMA
                     ELHATLTKKGVVSALREGYLRFSPHLNVAEGDLERFEQQISPTA"
     gene            complement(4668..5729)
                     /gene="bioB"
                     /locus_tag="PLF85_10635"
     CDS             complement(4668..5729)
                     /gene="bioB"
                     /locus_tag="PLF85_10635"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNE20167.1"
                     /translation="MNPKAQTIDTRKFSEAAPLTDREEARRYLNGELPLYDLFARAAA
                     VREQYFGKAVRVHILDNIQNGRCPEDCGYCAQRRSAGSGVQEYPMKSEEEIFADAQAA
                     KDGGAYRFCMVTSGTGPSTNSTAKLSSLIERITNELGLKVCLSAGLVDPAKAQRLAEA
                     GLDRYNHNLNTSETHYGDICTTHTFADRVQTMETLSAAGVGLCSGVIAGLGETAADLV
                     EAAYALKALRVISIPVNFFIPVPGHAITNPQQLTPEYCLRALTVFRLINPDSEIRIAA
                     GREGHLRSLQAMALMVANSLFADGYLNVKGSNMRETLQLISDAGFVAEASGELSAPEA
                     APAYSADSLPGLLKYPKPG"
     gene            complement(5719..>6333)
                     /locus_tag="PLF85_10640"
     CDS             complement(5719..>6333)
                     /locus_tag="PLF85_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE20168.1"
                     /translation="MCRSRGILVLFDEVFTGLGRTGSFFAYEQAGVVPDLLALAKGLT
                     GGVLPLAVTLVSPQVHAAFLSNRAEHTFYHGHTMTGNPVGCAAALASLRIFDEEKRLT
                     QVQALESRMCELWKSIATRHATKIEDVRCRGAVSAVNLVSRAEASGYAFSWSKRLRAA
                     ALEQGVVLRPLGNVLYVAPPYNIEAQNLDRVFEVIDSLLTNYEP"
BASE COUNT         1375 a         1793 c         1703 g         1462 t
ORIGIN      
        1 ttagaaattc tgcccaacct ttttggtgaa tttttcacct cattggggtt gtagcaccga
       61 agacggagat cggcaagccg atcacagttg gtggtagcta caatggttct ggggttgtag
      121 ctcagttggt tagagcatcc gcctgtcacg tggaaggtca cgggttcgag tcccgccaac
      181 cccgcattca attgcgatcg tagaaaaatg gaaatccata gcatgctatg gatttccaaa
      241 actaccttaa acccgctttt cccctcgtct caccccggtc ttatgtcaca tggctaacga
      301 gctggcaaaa ggtctcgaat acctcaccgt gctcggtccc ggcaaggtca ttgtcctgct
      361 ttccaccatg cccggcgata aactcgccta cctgtcgcgg cacatcgagc ataagaagat
      421 tatccaggtc ctgcgcgcaa cctcagtcag gcaggtttcg gcaattgtga aaaagaccga
      481 tgtgcaggca cttgtaccac tcatgaatgt tctcgatgcc gacgagagcg tcgatctgct
      541 caaccggctc gagctcgaca ccattgtact ttttctgact caccttgacc cgcagcggct
      601 tgcacaaatt gtccgcggtg tcggggtgga aaagatgctc gagctgctcg ccgccaccgg
      661 caccgaacgc gcgctggtgc tggcgcgcga actgccttcg gcccaccttg tggccctcgt
      721 caagaaagtc ccgatgcgcg acctggcccc actggcagtc agtctcaggg tagagttact
      781 ctcgaacctt ttggccgctg acagtgcgga acatctggct caactcatca cgacacttgg
      841 ccaccgcaac ctgctcgacc tgatccactt catgggagac aaagagatca cgcacctgct
      901 gcagcgttac aaaggcccaa aacttgaagg gttgatcctg ctgctacacg gcatgcgcat
      961 caagagaccc aagcccgcga aaaagaaagc cgtgaagaaa aaaccggcaa agaaaaaaac
     1021 cgccaaaggg aaaaacaaac gcgcagcatg attgacaccc tgttgcagag ctaaagcaga
     1081 ttcagccgct tgctgttcaa ttcatacgtt tttcttttcc tgttctttcc ggtagtgctc
     1141 gccggctact acgcgctgcc acggccgcag tggcagctgg cgtttctgct gctgatgagc
     1201 ctcgtcttct acgcgtactg gagtatacca tacctgtttc tgctgattgt agtcgtggtg
     1261 cttgatttct ggctggcggc ggcaatccac cgcagtgaaa acacaggccg gcggcgtggc
     1321 ttattgatac tttcactggt tctgaacctg ggtctcctgt tcttctttaa atatgcgggg
     1381 ctggttgcaa caacgatcaa ccacttcgcc ccggtcttgc cggcaatttc tctggtgctg
     1441 ccgattggcc tgtcgttcta tacttttcag tcgttgtctt atgtcatcga cgtctatcgc
     1501 cgccattcaa gcgcgcaccc ttctctgcta tcgtattcaa cctatgtgac aatgttcccg
     1561 caccagattt cggggccgct ggtgcggcac aacacgatca taccacagct cgaagacggc
     1621 gccacctacc gcgtgcaaag cgataacctc tggcggggag cgtttttttt cgtcactggc
     1681 cttgcgaaga agatactcat cgccgatttt atctcgcgcg gtattaaccc gatcatcacc
     1741 aacatggcga cggcatcgaa cagcgaggca tggctggcga tggtgggtta taccctgcag
     1801 ctctacttcg acttcagcgg ctactcagac atggcgacgg ggctctgtct catgctcaat
     1861 atccgattac cacagaactt tgactccccc tacaaatcgg gttcgattac cgagttctgg
     1921 cagcgctggc acatgaccct ttcttcatgg ctccgggatt atctctacat ttcgctcggc
     1981 ggcaaccgcg gcacgaagtt tgccacctac cgcaacctga tgctgacaat ggccttgggc
     2041 ggcctctggc acggcgcaac ctggatgttt gcgctctggg gtatttacca cggcctgctt
     2101 ctggcaatag agcgcgccac gggcattggg cgcggcatta cgcccaatgc cttgcgcatt
     2161 gcgctggtct ttctgctagc ggcgctgggc tggattttat ttcgcgcccc cgacctttcg
     2221 attgcaacag tctggtacca gaaagtcttt ctgctgaacc cgacgctcag ctgggatctg
     2281 ctgtcgatac ccatcaagca ccgggaccgg ttcggcgtcg cttttcttgc agggctcgtt
     2341 atcgtctttg ccgcacgtaa cacctggcgc atcgcctttg ctccctcata ccgcaatgtg
     2401 ctgctgctcg tactgctttt tgctctgtgc ctgccgtttc tcggtgatga atcgccgttt
     2461 ctgtactttc agttctgacc atgagttcaa aaaaagccat tagccttttt accctgctga
     2521 gcgttgcggt gatagcagcg attgcagcct tcagctacac ggttgatcca ctctgctatt
     2581 accgctgcga aaatgttacg gcggagaaaa aaaatctcaa tggctaccac cgcaatctgc
     2641 agcgcattct gcgctacccc gatactgagc ttctggttct gggctcatcg cgcggcgaat
     2701 ctttgccgct tgcctggctg gcagaggagt atagtctcaa agcgctcaat ctttctgtcg
     2761 gcggcgccga catccatgcg aaagcagcat ttctgcaatt tgcactcgaa cgcctgcagc
     2821 tgaaaaaagt gatctggatt gccgattact ttgaacttat agacgaaaac atgagcgaca
     2881 aggtctcgct tactccggca ctgcgcaacc ttgccgcaaa atccgatacg ccggctttgg
     2941 gctcgtcact gaagcgcctg ccgactttga tcgaacataa cacaatcgag gccggtttcg
     3001 ctctgctgcg cggaaaaaaa gtccagtggc cgcccgaccg cggcgaaaac tcaggattgg
     3061 atcttccgca ctgccggcag tcggtaagcg atgcaaaact taccgaagaa aaattactca
     3121 aagaagtggg catgatctac gacggttatg ccggacgcat tctgctaccc gaacaaaacc
     3181 caaacagcta caagaaaatg cgcgagctgg cgcagactct ctcggcgcgc ggtatcagtg
     3241 tcataattgt gattccgccc taccatccgg aattcatgcg ccggctaaag gctgaacagc
     3301 cacagatttt ttcccgccat gaagtgtggg ctaagaagct gggcgctctg gccggtccca
     3361 atgctgaaat ccgggatttt ttctcaatgg cgcgattcgc ctctgcaccc gcgctccact
     3421 ggagtgacgg tgtgcacttc aactgcgcgg cagccgcaga gatgctttcc cgtaagcgat
     3481 aaattcccgt gaaaagggac gacgcgatat cgccttttgc cgctgcgctg ttgcatgata
     3541 gactatcgag caattcgcgc tcagtttgca gtgcgggaag atcttatctg gctcaacaac
     3601 tgcggagtga gcgtgccacc gaaaagttcc gcccaggcgg tgaccgagta cctggaagcg
     3661 ttcagcgcct cgggtattat gcaaacggcc aagccacacg gccaggtgaa gcgcaatata
     3721 cagtcactct tcgcaaagtt tctgggcggc tcacctcacg aatacgcgat tcttaacaac
     3781 acagcagaag gtatgacttt tatagcgcag agcctgccgc ttcagcgcgg agacaagatt
     3841 ttgcttgttg agcgagaata tcccagtaac gtctatccgt tcctgcagct gaaacggcaa
     3901 ggcattgaaa tcgggtatgt gaatcccggc ctgtcaaacg acgaatttct cgataatatt
     3961 tcaaagtcgc tcacgacgaa tgtgcgcgtg atgtcgcttt cggcagtcga ctggctgacc
     4021 ggcctgcgct ttgatctgaa ccgtatcggt cgccttctgc agagccgggg ggttgcattc
     4081 attctcgacg ctgcacaggg tgctggccat gtagaaattg acgtcaagga ggcaggcatc
     4141 gatgcgatgg ctttctcctg ctggaaatgg cttttgggcc ccctggggtc tggtggtctt
     4201 tacgtggcag acgatttcct gaagaaactc gatatccggg tggctggaac ttcttccgtt
     4261 aagaatgatg acacctacct gccgcaccgc gaagactata aagagagcgt cgagcgtttt
     4321 atgctctcaa ctgctcccta tatgaactgg gtctttcttg aatcttcact ccagttcatc
     4381 gacaccgtcg gcctcgaaac agtacagcaa cgcaacctgg agctcgcaga gcgtctttct
     4441 ggtatcttac gaaaggccgg gtacgaggtg ctgcgcgatc gttttggcca tgaccagacg
     4501 gctatcgtct gcgcgcggcg tgacggtgac atggccgagc tgcatgcgac gctgacaaaa
     4561 aaaggcgtag tgagcgcttt gcgcgagggt tacctgcgct tctcgcccca tctaaatgtc
     4621 gcagaaggcg acctggagcg gttcgagcag caaatttctc ctacggctta acctggtttt
     4681 gggtatttca agagacccgg cagtgaatca gccgaatagg ctggtgcagc ctcgggtgcc
     4741 gacaactcac cactggcttc ggccacaaaa ccggcgtcgc tgatcagttg cagcgtttcg
     4801 cgcatgtttg aacccttcac gttgaggtaa ccgtcggcga aaagcgaatt ggcaaccatg
     4861 agtgccatgg cctgcagtga gcgcaaatgc ccctcgcggc ctgccgcaat acgaatttca
     4921 gaatcaggat taatgagcct gaacacggtg agcgcgcgca ggcagtattc aggcgtgagc
     4981 tgctgcgggt tcgtaatggc gtgccccggg accggtataa agaaattcac gggaatagaa
     5041 atcacgcgca gtgctttaag cgcataggct gcctcgacca ggtctgccgc agtctctccc
     5101 agcccggcga tgacgccaga acagagacct acccccgcag cggagagcgt ttccattgtc
     5161 tgcacgcggt cggcgaaggt atgcgtggtg cagatatcgc cgtagtgggt ttctgaagta
     5221 ttgaggttat ggttgtagcg gtcgagccct gcttcggcga gccgctgcgc tttggcagga
     5281 tcgaccaggc ccgccgagag gcagaccttg agacccagtt cgttcgtgat gcgctcaata
     5341 agggaagaga gtttcgctgt ggaattcgtc gaaggccctg tgccggaggt caccatgcag
     5401 aaccggtaag ctcccccgtc tttggcagcc tgtgcatcgg cgaagatttc ttcttcgctt
     5461 ttcatcgggt attcctgcac acccgaacca gccgaacggc gctgcgcaca gtagccgcag
     5521 tcttcgggac agcggccatt ctggatgttg tcgagaatgt ggacgcgtac ggctttacca
     5581 aagtattgtt cgcgcacagc cgcggcgcgg gcaaagagat catagagtgg cagctcaccg
     5641 ttgaggtaac gccgcgcttc ttcgcggtcg gtcaggggtg ctgcttcgga aaatttacgg
     5701 gtatcaatcg tttgggcttt agggttcata gttggtaagc agggaatcga tcacttcaaa
     5761 tacgcggtcg agattctgcg cctcaatatt gtagggaggt gcgacgtaga gcacattacc
     5821 cagtggtcgc aggaccacac cctgctccag ggcagctgcc cgcaggcgtt tagaccacga
     5881 gaaggcataa ccagatgctt ctgcacgtga aaccaggttc acggcactca cagccccacg
     5941 acagcgaaca tcttctatct ttgttgcatg ccgggttgct atggatttcc atagctcgca
     6001 catgcgagat tcaagcgcct gcacctgggt taagcgtttt tcttcgtcaa atattcgcaa
     6061 cgaggccagt gcagccgcgc agcccacagg attgccggtc atggtatgcc cgtggtaaaa
     6121 ggtatgttct gccctgttgg agagaaacgc cgcatgcacc tgcggcgaga ccagcgttac
     6181 tgccagcggt agcactccgc ccgtaaggcc cttggccagc gcaagcaggt cgggaaccac
     6241 acctgcctgc tcgtaagcga aaaagctgcc tgtgcggcct aagccggtaa aaacttcgtc
     6301 gaaaagcacc agtataccgc gcgatcggca cat
//
LOCUS       DAOQJF010000192         6295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_6981_length_6295_cov_4.706410, whole genome shotgun sequence.
ACCESSION   DAOQJF010000192 DAOQJF010000000
VERSION     DAOQJF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6981_length_6295_cov_4.706410"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLF85_10645"
     CDS             complement(<1..908)
                     /locus_tag="PLF85_10645"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF020460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNE20169.1"
                     /translation="MNILLQNKIVARYAQIDEWLRSLEKDVPIFMSADIRDAGFKIAS
                     IDANLYPAGFNNLDASFHPLAARHLKDALAHYNIPAGSRLLLISEEHTRNSFYLENIR
                     ALSGLLAAAGYSVEVATQFAIADESYVDNALELRTASGNTVRLYDPAYASARLADYSA
                     LIMNHDSSAGTADWIKNATIPVLPAWQAGWHSRSKARHFAHYRTLMNELAAIVGVDPW
                     FFAPLDAHITGIDINSDADRAKVSEQAAGLLKQIQAKYDEYQIQEKPFVFLKSDHGTY
                     GMAMLSFDSAGEILEINRKEKNKLFKG"
     gene            complement(924..1235)
                     /gene="grxD"
                     /locus_tag="PLF85_10650"
     CDS             complement(924..1235)
                     /gene="grxD"
                     /locus_tag="PLF85_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNE20170.1"
                     /translation="MTLEEKKQEIDKDVKSNKVVLFMKGTKEFPQCGFSARVVQILNQ
                     HGASFVTRDVLSDPELRDAIKQYSEWPTLPQLYINGEFVGGCDIVTEMAQKNELAPLL
                     G"
     gene            complement(1232..1483)
                     /locus_tag="PLF85_10655"
     CDS             complement(1232..1483)
                     /locus_tag="PLF85_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNE20171.1"
                     /translation="MVDPQEVHRLIEAALPGARIQVLDPMRDGEHLQAVVAADAFVGL
                     PLVKQHKLVMEALREVLRDRLHALQLKTMTPSQYDKEYP"
     gene            1854..2222
                     /locus_tag="PLF85_10660"
     CDS             1854..2222
                     /locus_tag="PLF85_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE20172.1"
                     /translation="MFNSQMNIQQFVGRLVKDPTLKTTQSGKLLMVFPFAYNTYSKTD
                     ETGSTACFIDVEAWDKLADFQAPRLKKGMEVVIRGNLVQRRWKDQENKTRSAFKIVAR
                     AIIVSDLKRRPEPDHIREAA"
     gene            2288..3343
                     /gene="lipB"
                     /locus_tag="PLF85_10665"
     CDS             2288..3343
                     /gene="lipB"
                     /locus_tag="PLF85_10665"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNE20173.1"
                     /translation="MHLLRHPVSVTTQFIGLTAYEQALQMMEQAIAHSNGDTAYIWGL
                     EHPLVYTSGLKTDASHILSEAVQVVPARRGGSVTLHNPGQLVIYFVLPLAAVNGGLER
                     FVRVLEAVMAEALLSFGIAVNLLPGASGVFTPAGKIAFIGLGLKKGCIYHGASINVAN
                     NLADFRAIQSCGLTLSMTSMQAQLSGQMPSTADVFARFSGLLLERFSVISPAEFRSAA
                     VSRLDFIDWFTGFRLGWLAFHERRYWEAHELWELYWHEMPPGDLRIFFHALIQTAMAF
                     YKIFTVPNLAGAESLLAKALEKYAVTKGIRLLQEQEKFLEFLAGVLGALQNKKPVEDL
                     LPPVMSWVAEQPGLMQD"
     gene            complement(3345..4781)
                     /locus_tag="PLF85_10670"
     CDS             complement(3345..4781)
                     /locus_tag="PLF85_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNE20174.1"
                     /translation="MIDNYVRKLFTTFAGDQEIRIIIGVSYLVASVFFILGLKFMSSP
                     RTARRGMFMAEAGMALAIVTTLLDQEIVSLEWILIGLVIGSIAGYVMAVLTPMTAMPQ
                     RTALSHAFGALAAALVGVTKYYTGHVKPDDHGIMIAIGLEVLLGALTFTGSMIAFGKL
                     QGTLPGAPIQYKGQNVINVGIIAILLASFGYLVYNPGATELFYVLTVVALIFGVSLVL
                     PIGAADMPVVMSLLNAYAGLAGCATGFALNNNVLIIAGALDGASGVILSIIMTKAMNR
                     SMTNVLFGGFGAVAPVAEAREVKGSIKGQTAEEAAAILDIAESIIIVPGYGMAVSQAQ
                     HSCKKLVDYLIKKGKTVRFAIHPVAGRMPGHMNVLLAEAGVDYDLLCDMDQINDDFPT
                     TDVSIVIGANDVVNPAAKDDKASPIYGMPILNVDASRTVMVLKRGMSPGFSGIENELF
                     YKDNCVMVFGDAKKTLDGFVTGLQELDS"
     gene            complement(4778..5101)
                     /locus_tag="PLF85_10675"
     CDS             complement(4778..5101)
                     /locus_tag="PLF85_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNE20175.1"
                     /translation="MDILAPLYIFILAAFVGYNVIHKVPPLLHTPLMSATNAISAISV
                     VGAIIVAGMEHSGDTGVLGTIMAVVAVAAATTNVVGGFLITDRMLKMFKKDKPAAAEA
                     KEADK"
     gene            complement(5114..6241)
                     /locus_tag="PLF85_10680"
     CDS             complement(5114..6241)
                     /locus_tag="PLF85_10680"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNE20176.1"
                     /translation="MKLAVAKETTSGEKRVALIPESVKKLVAKGFSVLVEKGAGEGSY
                     ISDSEFQEAGATLVTAADFRSADAILKVRPPSTAEIDAYKDGGWIFAMLDPINRAENL
                     PAFEKKKTTAFAMEFIPRITLAQSMDVLSSMASIAGYRAVLMASQEFGRFFPMLMTAA
                     GTIPPAKAFVLGAGVAGLQAIATAKRLGAVVEAFDVRAAAAEQVESLGAKFVKMEITE
                     NLQDEQGYAKEASPEFIAKQKELLHKHIAKSDIVITTAQIFGKKAPILIEDYMVKSMK
                     PGSVIVDLASETGGNCTLTKHGENIEIGGVKILAPGNITSTMASAASRMFSKNMENLL
                     VYLFENGSLSKDFASDEILKRSVITRDGALVSDIVKKATNR"
BASE COUNT         1418 a         1675 c         1698 g         1504 t
ORIGIN      
        1 cccttaaaga gtttgttctt ctcttttcgg ttgatttcga gaatttcacc cgcgctgtcg
       61 aaagagagca tcgccatgcc ataagtacca tggtctgatt tcagaaagac aaaaggtttt
      121 tcctggatct ggtattcgtc atatttagcc tggatctgtt tcagcagccc ggctgcctgc
      181 tctgaaacct tcgcgcgatc agcatctgaa ttgatgtcga ttcccgtgat atgtgcgtcc
      241 agcggggcaa aaaaccaggg atccacaccc acaatggccg ccagttcgtt catgagcgta
      301 cggtagtgcg caaaatgccg tgccttgctg cggctgtgcc agcccgcctg ccatgccggc
      361 agcaccggta tcgtggcatt ttttatccag tcggcggtgc cggcagacga gtcatggttc
      421 attatcagtg ctgagtaatc ggcgagacgc gctgatgcat aagcaggatc gtagagccgt
      481 acagtattgc ccgaagctgt cctgagttcg agagcgttat ctacgtaact ttcgtccgcg
      541 atggcaaact gcgtcgcaac ttctaccgaa tagcctgccg cggcgagcag accgctcagc
      601 gcccggatgt tttcgagata aaagctgttg cgtgtatgct cttcgctgat gagcagtagt
      661 ctgctgcccg ccggtatatt gtagtgcgcc agggcatctt tcaggtgcct tgcggcgaga
      721 ggatggaagc tcgcatccag gttgttaaaa ccggccgggt acaggttggc atcaatcgaa
      781 gcaattttga aacccgcatc cctgatatct gccgacataa aaatcggcac gtctttttcg
      841 agcgaacgca gccattcgtc gatctgtgca tagcgcgcaa cgattttgtt ttgcagcaag
      901 atattcactt agagtggcgt cgctcagccc aacaggggag cgagctcgtt tttctgcgcc
      961 atttcagtga caatgtcaca accgccgaca aattcgccgt tgatatagag ctggggcagc
     1021 gtcggccatt cagagtactg cttgatggca tcgcgcagct cagggtcgct gagcacgtcg
     1081 cgcgtcacga aagaagctcc atgctggtta agaatctgta ccacgcgtgc agaaaagccg
     1141 cactgcggaa attcttttgt acccttcata aagagaacaa ccttgttact tttgacgtcc
     1201 ttatcgattt cctgtttctt ttcttcgagt gtcatgggta ttccttgtca tattgtgacg
     1261 gtgtcatggt cttcaattgc agcgcatgca ggcgatcccg cagaacttcg cgcagggctt
     1321 ccatcacgag tttatgctgt ttcaccaggg gtaatcccac gaaggcatct gctgccacga
     1381 cggcctgcaa atgctcaccg tcgcgcatgg ggtctaaaac ctgtatgcgc gcgcccggta
     1441 gggctgcttc gataaggcgg tgcacttcct gtgggtctac catttgaatg actgcaagac
     1501 aaatatatac ccgtcagcgc attttggcaa gaatatgggg gagttatcac gttcagtgcc
     1561 ggtaacgagt tcgcgggggc gaccgcagag attttgctct aaggaatgcc tgaaaaagtc
     1621 gcgaaccgcg ccgaataggc gcggacgttg cgcgagaaca ggccattcat ggccgttttg
     1681 cgagcgattt cgtcaggaaa tcgcggcgtc cttgcggaac aggcgacttt ttcagagtat
     1741 tcctaaaaaa aatgcaaaaa cggtgagaaa aagaccgggt ttgctggtat ttaatatgta
     1801 gaggggcaaa gtccctcaaa taaattgtga ccctaagggg taaaagagga agaatgttta
     1861 acagccagat gaatatccaa cagtttgtcg gccgcctggt caaggacccg acactcaaga
     1921 ccacgcagag cggtaaactg ctcatggtct ttccattcgc gtacaacacg tacagcaaga
     1981 ccgatgagac cggaagcacc gcctgcttta tcgacgttga agcctgggat aaactggcgg
     2041 attttcaggc tccgcgcctg aaaaaaggga tggaagttgt catccgcggt aatctggtgc
     2101 agcgccgctg gaaagaccag gaaaacaaga cgcggtcggc tttcaaaatc gttgcgaggg
     2161 ccattatcgt gagcgacctc aagcgtcgtc ccgaacccga ccatatccgc gaagctgcct
     2221 gacctgcaga cgacttacgg tttacccgtt gccgctgttt taaaacacag aggcaacggg
     2281 ttttttcgtg catctcctac ggcatcctgt ttcagtcact acccagttca taggtctcac
     2341 tgcctatgag caggcgctgc agatgatgga acaggccatt gctcattcaa acggcgacac
     2401 ggcttatatc tggggcctgg agcatccgct cgtctatacc agcgggctta aaaccgacgc
     2461 ttcgcacatc ttgagcgagg cggttcaggt tgtacctgcg cggcgcggtg gatcagtcac
     2521 gctgcacaat cccggtcagc tggtgatcta ctttgttctg ccacttgccg cggtcaatgg
     2581 cgggctcgag cgctttgtcc gggtgctcga agccgtgatg gcagaggcat tactcagctt
     2641 cggaatcgcg gtcaaccttt taccgggtgc cagcggggta tttacgccgg cgggcaagat
     2701 tgcctttatc gggctaggtc tcaagaaagg ctgcatctac cacggcgcct cgattaacgt
     2761 ggccaataac ctggccgatt ttcgcgccat ccagtcgtgc ggtctgacgc tttcaatgac
     2821 cagcatgcag gcacagttgt caggccagat gccttctacg gctgatgttt tcgcgcgttt
     2881 ttcgggcctg ctgctcgaac gctttagcgt aatcagtccc gccgagttta ggtcagcagc
     2941 cgtaagccga ctggatttta ttgattggtt cactggattc cggctgggct ggctggcatt
     3001 tcatgagcgc cgatactggg aagcgcacga gctgtgggag ctatactggc acgagatgcc
     3061 gcccggcgat ctgcggatat tttttcatgc actgatacag accgcaatgg cattctacaa
     3121 gatatttacc gtgcccaacc tggccggtgc agaatcacta ctggcaaaag cactggagaa
     3181 atacgcagtc acaaaaggga ttcgcctgct gcaggagcag gaaaagttcc tggaattcct
     3241 tgccggtgtg cttggcgcgc tacaaaataa aaaaccggtg gaggatctcc tgccaccggt
     3301 tatgagctgg gttgcagagc aacccgggct aatgcaggac tagatcagga gtcgagctcc
     3361 tgcaggccag tgacgaaccc atcaagggtc ttcttggcat cgccgaacac catgacgcag
     3421 ttgtctttgt aaaagagttc gttctcgatg cctgagaatc ccgggctcat accgcgcttc
     3481 agcaccataa cggtacgcga agcgtcgacg ttcaggattg gcataccgta gatcgggctc
     3541 gccttatcgt ctttcgctgc cgggttcaca acgtcgtttg caccaatgac gattgatacg
     3601 tcggtagtag gaaagtcgtc gttaatctgg tccatgtcgc agagcaggtc atagtcaaca
     3661 ccggcttcag cgagcagaac gttcatgtgg ccgggcatac ggcctgccac cgggtggatg
     3721 gcgaaacgca cggttttgcc tttcttaatc aggtaatcaa ccagcttctt gcagctatgc
     3781 tgtgcctgcg aaactgccat accgtaaccg ggaacgatga tgattgattc agcaatgtcc
     3841 agaatcgctg cggcttcttc cgcagtctgg cccttgatgg aacctttgac ttcacgtgct
     3901 tccgcaaccg gtgccaccgc gccaaaaccg ccaaacagga cgttggtcat cgagcggttc
     3961 attgccttcg tcatgatgat cgacagaatg acgccggaag ctccgtcgag tgcgccggca
     4021 ataatgagga cgttgttgtt cagcgcaaaa cccgtcgcgc aacccgcgag acccgcgtat
     4081 gcattcaaca gcgacatgac cactggcata tcggctgcac caatcggtag aacgagactt
     4141 acaccgaaga tcagggcaac cacggtcaga acatagaaga gttccgttgc accgggatta
     4201 taaacgaggt aaccaaatga agccagcagt atcgcaatga tacccacgtt aataacgttc
     4261 tggcctttat actggatcgg cgctccaggc agtgtgccct gtagcttgcc gaatgcaatc
     4321 attgagccag tgaacgtcag tgcgcccagc aggacttcaa gaccaatcgc gatcatgatg
     4381 ccgtggtcgt caggcttcac gtggcctgtg taatatttcg tcacaccgac aagagccgcg
     4441 gcgagtgcac cgaaagcgtg cgaaagcgcc gtccgctggg gcatcgcagt catcggcgtg
     4501 agaaccgcca tcacgtaacc ggcgatagaa ccaataacca gaccgatcag aatccattca
     4561 aggctgacga tttcctggtc gagcagcgtc gtcacaatgg cgagcgccat accggcttcg
     4621 gccatgaaca taccgcgccg cgctgtgcgc gggctgctca tgaacttcag gcccaggata
     4681 aagaaaactg aggcaacaag gtaagatacc ccgatgataa tgcggatttc ctggtcaccc
     4741 gcgaatgtag tgaagagttt gcgtacgtag ttatcgatca tttgtcggcc tcttttgctt
     4801 cagcggctgc cggtttgtct tttttgaaca ttttgagcat gcggtcggtg atgaggaatc
     4861 cacccacgac gttggtagtt gctgccgcaa cggctacaac cgccatgatg gtaccgagga
     4921 cgccggtatc accgctgtgt tccatgcccg caacgatgat agcacccact accgaaattg
     4981 ccgaaatggc attggtagct gacatgaggg gagtatgcaa cagtggcggt actttgtgga
     5041 tcacgttgta acccacaaaa gccgcgagga taaaaatgta gaggggagcc aagatatcca
     5101 tagtttctcc tgtttaccgg ttggtcgctt ttttgacgat atcagatacc agcgcaccat
     5161 cgcgggtaat gacgctgcgc ttgagaattt catccgaagc gaagtctttg ctgaggctac
     5221 cgttttcaaa gaggtatacc agcaggttct ccatgttttt cgagaacatg cggcttgccg
     5281 cactcgccat cgtcgaggtg atattgcctg gagcgagaat cttcacgccg ccgatttcga
     5341 tgttttcacc gtgtttggtc agggtgcagt tgccaccggt ctctgatgca aggtcaacaa
     5401 tgactgagcc cggtttcatg cttttgacca tgtagtcttc aatcagaatc ggtgcctttt
     5461 tgccaaagat ctgggccgtc gtaatcacga tatctgactt cgcgatatgc ttgtgcagca
     5521 gttctttctg cttcgcgatg aattcgggtg acgcttcttt cgcgtagccc tgttcgtcct
     5581 gcaggttctc ggtgatttcc atcttgacga acttagcacc aagactctct acctgttctg
     5641 ctgccgcagc acgtacatcg aaagcttcaa caacagcgcc gagacgtttt gccgtggcaa
     5701 tcgcctgcag accggcaaca cctgcgccga gcacgaaggc ttttgccggt ggaatcgtgc
     5761 cagctgctgt catcagcatc gggaagaaac gtccgaattc ctgcgatgcc ataagcacag
     5821 cgcggtaacc tgcgatcgac gccatggagc tcaggacgtc cattgactgc gccagcgtga
     5881 tgcgcgggat aaattccatt gcaaatgctg tggtcttctt cttttcgaag gcaggcaggt
     5941 tctctgcgcg gttgatagga tcaagcatcg cgaatatcca gccaccgtct ttgtaggcgt
     6001 cgatttctgc cgtggagggc ggacggactt tgagaatcgc gtcagcagat ctgaagtcgg
     6061 ctgctgttac gagtgttgcc ccggcttcct ggaattcgct gtctgaaatg tacgagcctt
     6121 cacccgctcc tttctcaaca agaacgctga accctttggc taccagtttc ttgacacttt
     6181 caggaatcag cgctacccgc ttttccccgc ttgttgtttc ttttgctact gcaagcttca
     6241 tgcgcgtgct ctccgtttca taaagttatc ccagatcact acgatcgggg ctgct
//
LOCUS       DAOQJF010000193         6275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7008_length_6275_cov_5.109164, whole genome shotgun sequence.
ACCESSION   DAOQJF010000193 DAOQJF010000000
VERSION     DAOQJF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7008_length_6275_cov_5.109164"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PLF85_10685"
     CDS             complement(<1..1222)
                     /locus_tag="PLF85_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20177.1"
                     /translation="MGLRERASDFSKETRENRGLDLGLELRPGVPGTAIQAPQPAPGK
                     AEPLEKKTPPLPALPSLSEFPETTGHKARLDSILNLIEIYKEFGSVRSAADLWQVIAY
                     SLMAQLGAKNIAIFMEDDDRMHLMHALGFTLASDYSFSGKNRLCRELGETRRIVFTDD
                     ILGSLPENEAKLLSASGARYAAPVFRYEELRGVILINPPAGRAKFSDDDLFYLKICGE
                     LLGAMEAQLRLVAGAEQRNEHLQRAQKYRGYARDFSVALGQLDNHEPLKDALENEMLQ
                     HFSESPLLLMLREDFFLRAHFATGYPRDSVQHLEVPLVEPLVEKIKQGQRTFTTTDFD
                     SSESFTFLGRYRQVWAHPILHRREFLGIAFIADQDAERVDALGTIVTQYVLQNHVSKL
                     RDHATSSLAHADNPV"
     gene            complement(1226..3052)
                     /locus_tag="PLF85_10690"
     CDS             complement(1226..3052)
                     /locus_tag="PLF85_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20178.1"
                     /translation="MSEAVAEKKQVRDDEFDIHGNLATQEGDDSFVVLYDSAEDKTGP
                     AEPVSPSLEDLNEKQVMAAPETSQAHPEDAYIDDILSSDDLEPVAPIAGVQQAADDED
                     GPIALTNEELDGILEMGPATEEEFHPDFPVAAENEALTPPPLPEDMDVEGFASPRMDG
                     SKPEDMDVEDERTVAPLSAEPATFDDEELPIAISDEDLDEILLEEEGGELPLPPADLN
                     EDEAALSSYEPAGADMTAAEPEAVLTEEEPIALSDDELDHILADAEAGHAEPAPELTP
                     HTEDEILTLDEEPELTLIDDTSSPLEAATEDMQPQEHAESGGFFDSDEDEPITLSDEE
                     LDGILSAAPEEENAPSDEVQLAEDVQQESHVVEDDEPIALSHEELDGILMDANEAPEE
                     EIPAAEEAPAPAESKGFFDSDEDEPIALSADELDGIAATATPEEHTPAPAEPSADFDS
                     RYADVDQMAEGTAEDELAVPAMESEEAEATAADDDEGPIALSADELDGIAADAHEVTP
                     EAPMVGLSPLDGSPAEPSLAEQTDKPATVFEEDLEAATLIHEDDRGAAPAAAPTPDTD
                     ELKSVMGYLDNLLGELPDDVIEKFAQSEYFKLYQKIMDKLGL"
     gene            3193..3393
                     /locus_tag="PLF85_10695"
     CDS             3193..3393
                     /locus_tag="PLF85_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20179.1"
                     /translation="MENHGGTPALSEAEGAFTASGRRGAISQRGLTYFNKDKHPFTNH
                     EHLVYFFGTTIATGVIFHRGEL"
     gene            3510..>6275
                     /locus_tag="PLF85_10700"
     CDS             3510..>6275
                     /locus_tag="PLF85_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20180.1"
                     /translation="MEFQAFEPKSETALADLFKDTPAIRAAFSTVNPNEFNRRLDKLM
                     QFDPVMGVKSMHALGALSKERPALPQLADSVSSVIDRFARYYQATPARQQEYNDAIDL
                     VNRLLDVDPKAITDGAYLLARMVRYAGESEPLFPLSVTHPDVTRVNEYHFPELSTWKV
                     CDDSGSVFLNSKPESDLTDMEGRDTLWGLYDILRTGFCIYANRSGPRIEITGDGAGAK
                     AEAVVSESIITGINVTNPGSGYTTAVVTIDSSPGPGSGATATATISGGQITGITVTAG
                     GSAYVPKSDNSPGAFMRNATAVLKNSGDVELRVRNIIYDLQNKLADYSASEKTIADWT
                     VNNSNVVFQAQTAVVDYMIDYVYPITRKDFVFNDGKEVLDREAEHLSMLNPDGATSTN
                     DKFLTEWLLKSLNADAEKLDNLENFSDVDTTSSLYVFGKDMVENPTYGINRVFGPMGY
                     PKPALKAKLMTGSTYTSNTTPPKDEEFGGLLSNGGPIWRLTQSKSTQAYLNPFRQFMS
                     TRVNALAPVPATYNNEFYFEAALKNFYFHILDRYYDQTNLQWALTTEEAQTSFADPER
                     NLQGYIAQMQYSMRNMAILDRTGKKPGDVGFNNVPYLTEFLYTIAAANGIVDPINAPA
                     QLTLQTCLYSMGATDIAADGVRTINLPCILGICLDPMDSQVTNKAIEHDIPASSRNDQ
                     PYSASLSMFAFELISPGRFIPRADSELYAADTVNGRFRGRFSPHQGDIESTNQKTSNW
                     MVAEFALNAWEGYGPFAVRGRAPNGSVVKYENDYYTDAYRSKMATDAGTYTTTALGTN
                     AGEGGLISGNGNYHIYERIYRPRNSGDPCWVDSQGSTYGYARYGYVRPENNQNYWDAS
                     NCASWEKVRIDFETRDEAIRANIEWVLKHKKYIFVIPMFGYTNMWTLIDNGAGFAVFS
                     TIHGNGLW"
BASE COUNT         1314 a         1718 c         1711 g         1532 t
ORIGIN      
        1 tgaccggatt atcagcgtgc gccagcgagc ttgtcgcgtg gtcgcgcaat ttcgaaacat
       61 ggttctgcag gacgtactgc gttacgatgg tgcccagcgc gtcgacccgc tcggcgtcct
      121 ggtcggcaat aaaagcgatt ccgagaaact cacgccggtg cagaatcgga tgcgcccaga
      181 cctggcggta gcgccccagg aacgtgaaac tttcgctgct gtcaaaatca gtggtagtaa
      241 acgtgcgctg gccctgcttg attttttcca ccagtggttc aaccagcgga acttcgagat
      301 gctgcactga atcgcgcggg taacccgtgg caaaatgcgc gcgcaggaaa aaatcttccc
      361 gcagcatcag cagcagcggg ctttcggaaa aatgctgcag catttcattc tcgagcgcat
      421 ctttcagcgg ttcgtggtta tccagttggc ccagggcgac agaaaaatcg cgggcatagc
      481 cgcggtattt ctgcgcgcgc tgcaggtgtt cattcctctg ctccgcgccc gcaacgagcc
      541 gcagctgggc ttccatggct ccgagcagct ccccgcagat tttcaggtag aacaggtcgt
      601 cgtcggaaaa ttttgcccgg ccagcgggtg ggttgataag tataacaccg cgcagttctt
      661 cgtagcggaa gaccggcgcc gcgtaacgcg ctccactggc gctcagcagt tttgcttcat
      721 tctcgggcaa cgaaccgagt atatcgtcag taaaaacgat gcgccgtgtc tcgccaagtt
      781 cgcggcagag tcggttcttg ccagagaacg aatagtcgct ggccagcgta aaacccagcg
      841 catgcatcag gtgcatacga tcatcatctt ccatgaagat cgcgatgttt ttcgcgccga
      901 gctgcgccat cagcgagtag gcgattacct gccaaagatc agctgccgaa cgcactgagc
      961 cgaactcttt gtagatttct atcaggttca gtatggaatc gaggcgcgcc ttgtggcccg
     1021 ttgtttcggg aaactcgctc aggctgggta acgctggtag tggaggagtt tttttctcga
     1081 gtggttccgc tttacccgga gcaggctgtg gtgcctgtat tgcagttcct ggtactcccg
     1141 ggcgcagttc taacccgagg tcgagtccgc ggttttcccg tgtttctttg gagaaatcgc
     1201 tggcgcgttc gcgtaaaccc atgggctata gcccaagctt gtccataatc ttctggtaaa
     1261 gcttaaaata ctctgactgg gcaaacttct caatcacgtc gtccggtagc tcgccaagca
     1321 ggttgtccag gtaacccata accgacttga gttcatcggt gtcgggcgtc ggcgctgcgg
     1381 ctggtgctgc gccacggtcg tcttcgtgga taagggtcgc ggcctcgaga tcttcttcaa
     1441 agaccgtcgc aggtttgtcc gtctgttctg cgaggctcgg ttctgcaggc gaaccatcga
     1501 gcggcgacag gcccaccatc ggggcttctg gggtcacttc gtgtgcgtca gccgcaatgc
     1561 cgtcgagttc gtcggcagaa agggcaattg gaccttcatc gtcgtcggcc gcagtcgctt
     1621 cggcttcttc actttccatc gctggaactg cgagttcgtc ttcggctgta ccttctgcca
     1681 tctggtcgac gtcggcatag cgactgtcga aatcagcgga cggttctgcc ggggcagggg
     1741 tgtgttcttc aggcgtcgcg gtggcggcaa tgccgtcgag ctcatcggcc gagagcgcga
     1801 tcggttcatc ttcgtcgctg tcaaaaaaac ctttgctttc tgctggggcg ggtgcctctt
     1861 cggctgcagg tatttcttct tcaggcgctt cgtttgcatc catcaggatg ccatcgagtt
     1921 cttcgtgcga cagcgctatc ggctcatcgt cttcgacaac gtggctttcc tgctgcacat
     1981 cttctgccag ctggacttcg tcactcggtg cattctcttc ttcaggtgct gcactcagta
     2041 tgccgtcgag ttcttcgtcc gaaagggtaa tgggctcatc ttcgtcgctg tcaaagaatc
     2101 cgccgctttc tgcatgttcc tgcggctgca tgtcttctgt ggcagcttcg agcggtgatg
     2161 aagtgtcatc aatgagcgtc agctcgggct cttcgtcgag ggtgagtatt tcgtcttcag
     2221 tatgcggagt cagctctggg gcaggttctg catggccggc ttctgcatca gccaggatgt
     2281 gatccagttc gtcgtcagac agcgcgatgg gctcttcttc tgtcagtacg gcctctggtt
     2341 cggccgcggt catgtctgcg ccagccggct cgtaagagga gagcgccgct tcatcttcgt
     2401 tgagatcggc cgggggcagg ggaagctccc caccttcttc ttcgagcagt atttcgtcga
     2461 gatcttcgtc agagatggca ataggcagct cttcgtcgtc gaatgttgcg ggctcggctg
     2521 agagaggtgc tacggtgcgc tcatcctcga catccatgtc ttcgggtttg ctgccgtcca
     2581 tccgtggact tgcaaacccc tcgacatcca tgtcttcggg aagcggtggg ggagtcagtg
     2641 cttcgttctc ggctgctacc gggaaatccg gatggaactc ctcttctgtg gctggtccca
     2701 tctcgagtat accgtcgagt tcttcattcg tcagcgcaat cgggccgtct tcgtcgtcag
     2761 ctgcctgttg taccccggca atcggggcaa ccggttcaag gtcgtctgat gaaagtatgt
     2821 cgtcgatata ggcatcttca ggatgcgcct ggctggtttc gggtgcggcc attacttgtt
     2881 tttcattgag atcttcgagg ctgggtgaga caggttctgc tggccctgtt ttatcttccg
     2941 ctgaatcata gagtacgaca aaagagtcgt ccccctcttg tgtggccagg ttaccatgaa
     3001 tatcaaattc atcgtccctg acctgctttt tttccgcaac agcctctgac attttttaca
     3061 ccccatttat tatcggttca ttaggcgcac aatcttaaat tagcgggact cgcgcgcctg
     3121 ccgcgacaca ccgtgcagtc ctcaagcagg tgatttgccg cagcgggtgt gtgaaatgcc
     3181 cggcggagcg gtgtggaaaa ccacggcggc acgcctgccc tgagcgaagc cgaaggggct
     3241 ttcacggcga gcgggcgtcg tggcgctatc tcgcaaagag gcctgactta ttttaacaaa
     3301 gataaacatc cgttcacaaa ccatgaacac cttgtttatt tttttggcac aactatagcc
     3361 acaggtgtca tttttcatcg gggagagcta taagctcgat gaaaagggga aacgtgaaca
     3421 aaaaccgtaa ctttcagccg aatcgatcct tgaaactggt ctttttgctg ttattttcgc
     3481 taagttgggc ggcttgtaag cccagcgagt tggaatttca ggcgtttgaa cctaagtccg
     3541 aaactgcact tgcagacctc tttaaagata cccccgcaat ccgtgctgct ttctcgactg
     3601 ttaacccgaa cgaattcaac cgccgcctcg ataagctgat gcagtttgac ccggttatgg
     3661 gtgtcaaatc aatgcatgcg ctcggtgcat tgtctaaaga gcgtccggcg ctccctcaat
     3721 tagccgattc tgtttcttct gttatagacc gttttgcccg ctactaccag gcgacgccag
     3781 caaggcagca ggaatacaac gacgcgatag acctggttaa ccgcttgctc gatgtcgacc
     3841 ccaaggcgat cactgatggt gcgtacctgc tcgcacgtat ggtgcgttat gctggtgaga
     3901 gtgaaccgct gttcccgttg agcgttacac accccgatgt cactcgagtt aatgaatacc
     3961 acttccctga actttccacg tggaaggtct gcgacgactc tggctcagtc tttctgaatt
     4021 cgaaacccga atctgacctg accgacatgg agggacgcga caccctctgg ggcctttacg
     4081 atatcctgcg tactggattc tgcatctacg ccaaccgctc aggccctcgt atcgagatta
     4141 ccggggatgg ggcgggcgcc aaggctgaag ccgtggtttc cgagagtatc attaccggca
     4201 tcaatgtgac caaccccgga tcaggctaca ccaccgccgt ggtgacaatt gactcttcgc
     4261 cggggccggg ttctggggcc accgcgacgg cgaccatatc tggtggccag ataactggca
     4321 ttacggtaac cgctggcggt tctgcttatg ttcctaagtc cgacaactcg ccgggtgctt
     4381 tcatgcgcaa tgcgactgcc gtgctcaaga attccggcga cgttgagctg cgcgtgcgca
     4441 acattatcta cgacctgcaa aataagctgg cggattactc cgcgagtgaa aagaccattg
     4501 ccgactggac agtcaacaac tcaaacgtcg tattccaggc acagacggcc gtggtcgatt
     4561 acatgatcga ttacgtttac ccgatcacgc gcaaagattt tgtttttaac gacgggaaag
     4621 aggtactcga ccgtgaggcc gagcacctgt cgatgctgaa ccccgacggg gcgacttcga
     4681 caaacgacaa attcctcacc gaatggctgc tcaagtcact caacgccgac gcggaaaagc
     4741 tcgataacct cgaaaacttc tctgatgtcg atacgacgag ttctctttac gtgttcggta
     4801 aagacatggt ggaaaatccg acttacggca ttaaccgcgt ttttggcccg atgggctatc
     4861 cgaagccggc actcaaagcc aagttaatga ccggttccac ctatacgagt aacacgacgc
     4921 cgccgaagga tgaggagttt ggtggtcttc tgtccaatgg tggtccaatc tggcgcctga
     4981 cgcagtccaa atcgacgcag gcatacctga accctttcag gcagttcatg tcgacgcgcg
     5041 tcaatgcgct cgcgccggtg ccagccacgt acaacaatga gttctatttt gaagcggccc
     5101 tgaagaactt ctatttccat atactcgatc gttactatga ccagacgaac ctgcagtggg
     5161 cgctgacaac agaagaggcg cagacttctt tcgccgaccc cgagcgcaac ctgcagggct
     5221 atatcgcgca gatgcagtac tcgatgcgca acatggcgat tctcgaccgt acaggcaaga
     5281 aaccaggtga tgtcggtttc aacaatgtgc catacctgac tgagtttctc tacactattg
     5341 ctgcggccaa tggtatcgtc gatccaatca acgcaccggc acagctcacg ctgcagactt
     5401 gtctctattc aatgggggca acggatattg cagcggatgg tgttcgcaca atcaaccttc
     5461 cgtgcattct cggtatctgc ctcgatccga tggattcaca ggtaacgaac aaggcgatcg
     5521 aacacgatat tccggcgtcg tcacgcaacg accaaccata tagtgccagt ctgagcatgt
     5581 ttgcctttga gctcattagt ccgggccgct ttataccgcg cgccgacagc gaactctacg
     5641 cagcagacac tgtcaatggt cgttttagag gccgattttc gccacatcag ggcgacattg
     5701 agtcaaccaa tcagaaaacc tccaactgga tggttgctga gtttgcactc aatgcctggg
     5761 aaggttatgg tccctttgct gtacgcgggc gggcccccaa tggcagtgtg gttaagtatg
     5821 aaaatgacta ctacaccgac gcctaccgct ccaaaatggc cacggatgct ggtacttaca
     5881 cgacgactgc cttgggtacg aacgcaggag aaggtggtct tatctcagga aatggcaact
     5941 accatatcta cgaacggatt taccgtccac gcaattcagg tgatccctgc tgggtagatt
     6001 ctcagggcag cacgtatggc tatgcgcgct atggctatgt gcgtcccgaa aataaccaga
     6061 actactggga tgcaagcaac tgcgcgtctt gggagaaagt gcgtattgat tttgaaaccc
     6121 gcgacgaggc aatccgggca aacatcgaat gggtattaaa gcataagaag tacatttttg
     6181 ttattcccat gtttggttat accaacatgt ggacgctcat tgacaatggt gcaggttttg
     6241 cggtcttttc cacaattcat ggtaatggac tttgg
//
LOCUS       DAOQJF010000194         6231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7093_length_6231_cov_5.223769, whole genome shotgun sequence.
ACCESSION   DAOQJF010000194 DAOQJF010000000
VERSION     DAOQJF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7093_length_6231_cov_5.223769"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..233
                     /gene="rplS"
                     /locus_tag="PLF85_10705"
                     /pseudo
     CDS             1..233
                     /gene="rplS"
                     /locus_tag="PLF85_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            385..1044
                     /locus_tag="PLF85_10710"
     CDS             385..1044
                     /locus_tag="PLF85_10710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNE20181.1"
                     /translation="MARFFFEPDDHSYRAVIGVDEVGRGALFGPVTVGAVLLSREAWA
                     VLPGESWFGSVKDSKLVKKAEREKLAPLLEAALPCALAHVSVRYIDRYNINAAIRYGI
                     YRAVQTLLRRTTFAAHEIRIIADGNYRFEYPALGMAKPMPRLDAYIKADQKYFPVSAA
                     SIVAKVRRDTLVERAAERFPGYGLDRHAGYGTAAHRTAIMQLGETAFHRKSFRGKAKP
                     P"
     gene            1104..3242
                     /locus_tag="PLF85_10715"
     CDS             1104..3242
                     /locus_tag="PLF85_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE20182.1"
                     /translation="MPATALTIPRRTRFRLSLLVAIPALVLLTGGLIVFFTLSNTRRT
                     MEDFGWALFREATKESINETRNIIREAIPDIETLGNLWRKNLFSRDMNWQARYFTELL
                     RSHREYSWVSFGDERGTFSGAYRPDTAGAPPRLNVSWLNNGRTISYDYEVLPNGQQKL
                     YKFEPDTGYDPRKRPFYQLAQKAGKRVWTPPYIFYEQAVPGITCSLPVYTGSRLEGVF
                     SIDFDLNALSDIVARARVSQRGVVFIYTENGEILGHPTMKVVSKAGQRAEGRILTVSD
                     IQDENIKAFFAALRQDQKDSPNGSGETSRNFHVKIEGDRFLASYSRFAIDDGLFWNIG
                     IIAPESDFRGIVEVRENYKRAYAISFAAILLSGMLAWLFTRMISSPLRSISLDMERVG
                     RLEIESGAANSSPFKEISLMDEHLQNMKGGLRSFASYVPKDLVRDLLKSGKEARLEGS
                     TETLTVLFTDIAGFTSFAEQLEPDELVQKLGKYFEIMTVQIMKNRGTIDKFIGDGIMA
                     FWGAPAADQNHAIGACLAALACKEALRQMAESPEYGHWARHLSTRFGIATGPVVVGNI
                     GTRERMNYTVMGDTVNLASRLEGVNKVYGTAISASDSTYNLVRAQIVGRTLDLVAVKG
                     KSHGVRIFEILCRRDGASAADLELEKISEQCLDAFIARDMATAKKFCLRILELRADDK
                     SANLLLRRIAEYEGLEDQKSWSPVNAMLTK"
     gene            3320..4246
                     /locus_tag="PLF85_10720"
     CDS             3320..4246
                     /locus_tag="PLF85_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNE20183.1"
                     /translation="MQTQSPSAVSHSATAGQNNPPLQKILIAGGGAMGAGIAYIFARS
                     YPEAQVDVMDTNEKVRIQAAESFEKWAAKDKERDEKAGKTFVDTNGRLNILAGFGKAQ
                     LHYDLMLEAVTENIEVKKKIFNEFDLRAHDNSLFASNTSSLSITTLSTAVNPRRQGRV
                     VGLHFFNPPRVMKLLEIITTEATEPSALALAREVAARLDKTAVVCKDAPGFITSRLGI
                     VLLNEAIFALQEGLMTAEEIDTSMKLGYNFPMGPLQLADFIGLDVVFAVTQTLYENYQ
                     DTKYRPCLLLRKKVEAGHLGQKSGRGFYVYDR"
     gene            4236..4715
                     /locus_tag="PLF85_10725"
     CDS             4236..4715
                     /locus_tag="PLF85_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20184.1"
                     /translation="MTADSLIAEFRSMSEAELERRFCGEAAAPALSPGDYTGTWLTRI
                     ENAGKYKPFNFLSQWLMFDLTPFGISFRGDGSGIWYFFDPRFAAGEFVTARSRSRWRD
                     TDCLAMDYGQANLPGFVKDLLYDEIKVLSPQHAIGIGGFNQPAGEGDNFYFLLTRKN"
     gene            4719..6119
                     /locus_tag="PLF85_10730"
     CDS             4719..6119
                     /locus_tag="PLF85_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNE20185.1"
                     /translation="MLIAGVTAYLVVTLLIGFWASRRVRDHRDFALAGKHLSYFMATA
                     TVFATWFGSETILGSSANFVQSGFFGVIEEPFGAALCLILVGALFARGLYRMNLTTFG
                     DFFRVRYGALAEVVASVILVFSYVSWVAAQFLALGIVFHTITGVSLEMAIVAMGLLVT
                     LYTTMGGMWAVSLTDTLQMTLIVIGLAAVFFYLLQQAGGWNAVMVNVPQSHFSFLPQG
                     KVSWLQYFSLWIVLGLGSIPGQDVFQRVMSSKNERVAAVSSIVAGLLYLTVAMIPLFL
                     GLVAVRLLSKGELPADMQFLLPQLIQLKTPVWVQILFFGALVSAILSTASGALLAPSV
                     LVGENLLARFVRDEKKLLLLSRISTVAIALWALQMTLANRHIHNLVTESSAIGLVALL
                     LPFLFGFYARRHNATTAVASMFLGFAVWQFWPGASVLFATDETQMPAVIAGLLASAVP
                     YFCEYSFRALQFVLRR"
BASE COUNT         1311 a         1697 c         1759 g         1464 t
ORIGIN      
        1 agaaagagcg tattcaggtc tacgaaggcc ttgtcattgc tatccaaaac cagggttcag
       61 gtaagacatt taccgtccgc cgggtaagct tcgacgtcgg tgtagagcgt attttccctc
      121 tctatgcgcc cacagtcgac aaagtagaga agatccgctc gagcaaggtt cgccgctcga
      181 agctctatta cctgcgtggc aagtcgggca aagacagccg cctgaaagaa atcgtccgca
      241 agccggtcaa agacccgctt ttcgcgaagg cacaggcata taaggcatct aaaccgaagc
      301 cggctgaagc tgcacctgcc gcagaagccg cggcggaaac tcctgcttaa cctttcaccg
      361 caggaaaccg aagagaaccg cgccttggcg cggttttttt ttgagcctga cgaccactct
      421 taccgtgcag tgatcggtgt tgatgaagtg gggcggggag cgctcttcgg gccggtgact
      481 gtcggtgcgg ttctgctcag ccgcgaggcg tgggcggtcc tgcccggcga gtcatggttt
      541 ggctcagtca aagattctaa actcgtcaaa aaagccgagc gtgaaaagct cgccccgttg
      601 cttgaggctg cgttgccctg tgccctggcg catgtttcgg tgcgctacat tgatcggtac
      661 aatatcaacg cagcgatacg ctacggtatc taccgcgcgg tacagacgct gctgcggcgt
      721 actacgttcg ccgcgcatga aatccgcatt atcgccgatg gcaattaccg ctttgaatac
      781 ccggctttgg gtatggcgaa acctatgccc aggctcgatg cctatatcaa agcagaccag
      841 aaatactttc cggtatcggc agcgtcgatt gtggccaagg tgaggcgcga cacgctcgtc
      901 gagcgtgccg cggagcgttt tcctggttat ggtctcgatc gccatgcggg ctacggcacg
      961 gcggcgcacc ggacggcaat catgcagctt ggtgaaacgg ccttccaccg caaatccttt
     1021 cgcggcaagg caaagccgcc ttagcagagc ctattgtctt tttcgcgtcg acagactctc
     1081 ttgctttgca gaacccgata ccgatgcctg ccacggcttt gaccatacct cgccgcacgc
     1141 gcttccggtt gagcctgctc gtggcgattc cggcactcgt tctgctcacc ggtggcttga
     1201 ttgtcttctt cactctatca aacacccgcc gcacgatgga agattttggc tgggcccttt
     1261 tccgcgaagc aacaaaagaa agtattaacg agacgcgtaa tattatcagg gaagcaatac
     1321 ccgatataga gacactcgga aatctctggc gcaagaacct gttcagccgc gatatgaact
     1381 ggcaggcccg ttactttacc gagcttttgc gctcgcacag ggaatacagc tgggtgagtt
     1441 ttggcgacga gcggggtacc ttttccggcg cgtacaggcc cgataccgct ggagctccac
     1501 cacgcctcaa cgtgagctgg ctcaacaatg gtcgtacgat cagttatgac tacgaagtgc
     1561 tgccgaatgg ccagcagaaa ctgtacaagt ttgagcccga cacgggttat gatccgcgca
     1621 aacggccatt ctatcagctt gcgcaaaagg ccggcaaaag agtctggact cccccctata
     1681 ttttttatga gcaggcagta ccgggtataa cctgttcgct gcccgtctac actggtagtc
     1741 gcctcgaggg tgtgttttca atcgactttg atctcaacgc cttgtcggat attgttgcgc
     1801 gggcgcgcgt cagccagcgc ggcgtcgtct ttatctatac agagaacggt gaaattctcg
     1861 ggcaccccac gatgaaggtc gtgtcaaaag ccggccagcg tgctgaaggt cgtatactga
     1921 cggtctcgga tatccaggat gaaaacatca aggcattctt tgcggcgctg cggcaggacc
     1981 agaaagattc gccaaacggt tcaggcgaga cgagccgtaa ttttcacgta aagatcgaag
     2041 gcgatcgttt tctagcgagt tattcgcgtt tcgcgatcga tgacgggctt ttctggaata
     2101 tcggcatcat tgctcccgaa agtgacttcc gcggtatcgt cgaagtgcgc gaaaactaca
     2161 agcgcgccta tgcgatttct tttgccgcaa tattactctc gggaatgctt gcgtggcttt
     2221 ttacccgcat gatttcttcg ccgctgcgct ccatttcgct cgatatggag cgggtgggca
     2281 ggctcgagat cgagagcggc gcagctaata gcagtccgtt caaagaaatt tcgctgatgg
     2341 acgagcacct gcaaaacatg aaagggggat tacgttcttt cgccagttac gtgccgaaag
     2401 acctggtacg cgacctgctc aaatcgggca aagaagccag gctcgaaggt tcgaccgaga
     2461 cgctgacggt attgtttacc gacatcgccg gcttcaccag ttttgccgaa cagctcgaac
     2521 ccgacgagct ggtgcagaaa ctgggcaagt atttcgaaat catgaccgtg caaatcatga
     2581 aaaacagggg cacgatcgac aagttcatcg gtgatggcat catggcattc tggggagcgc
     2641 ctgccgcaga ccagaaccac gcgatcggtg cctgcctggc tgcgctggcc tgtaaagaag
     2701 cgctgcggca aatggctgaa tcgcctgaat atgggcactg ggcgcgtcac ctgagcacac
     2761 ggtttggtat tgcaacgggc ccggttgtcg tcggaaatat cggtacgcgc gagcggatga
     2821 actacactgt tatgggcgat actgtgaacc tcgcctcgcg gttggaaggt gtgaacaaag
     2881 tctatggcac tgctatttcc gcgagcgatt ccacgtacaa cctggtgcgc gcgcagattg
     2941 taggccgcac gcttgacctg gttgcggtaa aaggaaaatc gcacggtgtg cgaattttcg
     3001 agatactgtg ccgccgcgat ggcgccagtg ccgccgatct cgagctggaa aaaatttcgg
     3061 agcaatgtct cgatgctttt attgcccgcg acatggcaac tgccaagaag ttttgcctgc
     3121 gcatactcga actgcgcgca gatgacaagt ctgcgaacct gctgctgcgc cgtattgccg
     3181 agtatgaagg gcttgaagac cagaagagct ggagtccggt aaacgcgatg ttgaccaagt
     3241 gagcgccagc cggccgaaaa ctggcagacc cggtaccatc gaagcgttgt cgccctaagt
     3301 gacagttttc gactggcgta tgcaaaccca gagcccatcg gctgtttctc atagcgcaac
     3361 tgccggacag aataacccac cactgcaaaa gattttaatc gccggtggcg gtgctatggg
     3421 ggcaggcatc gcctacatct ttgcccgttc gtaccccgaa gcgcaggtcg atgtcatgga
     3481 caccaacgaa aaggtgcgca tccaggctgc cgagagtttc gagaagtggg cagcgaagga
     3541 caaagaacgc gacgagaagg ccggtaagac atttgtcgac acgaacgggc gattgaatat
     3601 ccttgccggc tttggcaagg cgcagctgca ctacgacctg atgctcgaag cagtgaccga
     3661 gaacatcgaa gtgaagaaaa agatctttaa cgagtttgac ctgcgcgcgc atgacaactc
     3721 gctcttcgcc agcaatactt cttcattatc gattacgacg ctttcgacag ctgtcaaccc
     3781 ccgccgccag ggtagggtcg ttggcctgca ttttttcaat ccgccgcgtg tcatgaaact
     3841 gctcgagatt atcacgacag aagcgaccga accctcggcc cttgcgctgg cccgcgaagt
     3901 cgctgcccgt ctcgacaaga ctgcggtcgt ctgcaaagat gccccggggt ttatcacctc
     3961 gcgcctgggt attgtgctgc tgaacgaggc aatctttgcg ctgcaagagg gattgatgac
     4021 ggcagaagaa atcgacacgt ccatgaagct tggctataat ttcccgatgg ggcccctgca
     4081 gctggctgac tttatcggtt tggatgtagt ctttgccgta acgcagacac tctacgaaaa
     4141 ctaccaggat acaaaatacc gtccttgcct gctcttgcgt aagaaggttg aagcaggtca
     4201 tttgggccag aaaagcggcc ggggatttta cgtctatgac cgctgatagc ctgatcgccg
     4261 aatttcgttc gatgagcgaa gcggagctcg agcgccgctt ctgtggcgag gctgcagcac
     4321 ctgctctctc gcccggcgac tatacgggca catggcttac ccgtattgaa aatgcaggca
     4381 agtacaaacc attcaacttt ctcagccagt ggctgatgtt cgacctcaca ccctttggaa
     4441 tttccttccg cggcgatggt agcggcatct ggtacttttt cgatccgcgc tttgccgccg
     4501 gtgaatttgt gacggccaga agccggtcac gctggcgcga tactgactgc ctcgcaatgg
     4561 attatggcca ggcaaacctg ccgggctttg tcaaagatct gctgtacgat gaaatcaagg
     4621 tgctttcacc gcagcacgcg atcggtatcg gcggttttaa tcagccagca ggcgaaggcg
     4681 ataatttcta tttcctgctg acacggaaaa attagggaat gctcatcgcg ggcgtcacag
     4741 cgtacctcgt cgtgacgtta ctgatcggct tctgggcgtc gcgccgtgtg cgtgatcacc
     4801 gggacttcgc gctcgcgggc aaacacctgt cttacttcat ggccaccgcc acggtttttg
     4861 cgacctggtt tggctcagaa acaatactcg ggtcttccgc caactttgta cagtccgggt
     4921 ttttcggcgt tattgaagaa ccgtttggtg ctgcgctctg cctgattctg gtgggggcct
     4981 tgttcgcacg cggtctctac cgcatgaacc tcaccacgtt cggtgatttt tttcgcgtcc
     5041 gctacggtgc gctggcagag gtggtcgctt cagtaattct cgtcttttcg tatgtcagct
     5101 gggttgcagc ccagtttctt gcattgggta tcgtctttca tacgattacg ggcgtatcgc
     5161 tggaaatggc gatcgttgcc atgggtctgc tggtgaccct ctacaccacc atggggggaa
     5221 tgtgggctgt ttctttgact gataccctgc agatgacgct gattgtcatc gggcttgccg
     5281 ccgtattttt ctatcttctg cagcaggccg gtggatggaa tgcggttatg gtcaacgtgc
     5341 cgcaatcgca tttctctttt ctaccgcagg gtaaagtatc gtggctgcag tatttttcac
     5401 tctggatcgt gctcggtttg ggctcgattc ccgggcaaga tgtctttcag cgggtgatgt
     5461 cgtccaaaaa cgagcgagtc gccgccgttt cgtccatcgt tgcaggcctg ctctatctga
     5521 cagtggcgat gattccgctt ttcctggggc tcgtagccgt gcgcctgctc tcgaaaggcg
     5581 agctgccggc agatatgcag tttttgctgc cgcagctgat acagctaaag acaccggtct
     5641 gggtgcagat cctttttttc ggagcccttg tttcggcgat actgagtacg gcgagcgggg
     5701 cgctgctggc accctcggta ctggtgggcg agaacctgct ggccaggttc gttcgcgacg
     5761 aaaaaaaatt actgctctta tcgcgtatca gtacggtagc gattgcgctc tgggcattgc
     5821 aaatgaccct ggcgaaccgg catatccata acctcgtcac cgaatcttca gcgatcggcc
     5881 tcgtcgccct gctgctgccc ttcctgttcg gtttttatgc ccggcgtcac aacgccacta
     5941 ccgcagtggc cagcatgttt ttgggttttg cggtctggca gttttggccg ggcgcatctg
     6001 tattatttgc caccgatgaa acgcagatgc ccgcggtcat tgccgggctt ctggccagtg
     6061 cagtccctta tttctgcgag tactctttcc gggcactgca atttgtcctg cggcgttagc
     6121 cgtagctatt ggctcctttt tcaggagcca atagcaggtt ccggaaaccg cgcaaggctg
     6181 cagacaggcc atttatggcc gttttgcgag cgattcgcat cgcgaatcgc g
//
LOCUS       DAOQJF010000195         6188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7167_length_6188_cov_5.665417, whole genome shotgun sequence.
ACCESSION   DAOQJF010000195 DAOQJF010000000
VERSION     DAOQJF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7167_length_6188_cov_5.665417"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLF85_10735"
     CDS             <1..426
                     /locus_tag="PLF85_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20186.1"
                     /translation="YKWPIFAQVSLHGGYGRRDYNRDGQAVPVRLIAGELVPALGYSF
                     GLRWFRLEPFAGFGLGYSNLQLDYSGLARKPVDTTAGLALSTQTIDQFYFLSQAGFIF
                     KYAPHPRWAIIVSPSATVFHFAGGAQAELNARMGVGFYF"
     gene            426..1385
                     /locus_tag="PLF85_10740"
     CDS             426..1385
                     /locus_tag="PLF85_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20187.1"
                     /translation="MQQRILQGGIICVTVVVLASGCIAQKPLDVTFTEGGKGAGIASG
                     LVEKTATATATVSSIANSIGSSVPILEFTVKENGSKDGYPLQIRELRFTNIGTGDAAD
                     FRFILDGPGSNNTIGFSTGQTVVFRDFGDIIIPDGETSGRTYQVKVHVRENIQGSTTD
                     NQTVQLKTSPTTDFDVTETSSNLLSTNGAFQQTSGTVMQVVATHLRGGGTFQFATVAR
                     NTDFPGTQTIYAQDVNQRVDKDFTYAIQLAPRTGALCASAAAGALSSTDGGLNKAAVQ
                     GVATWTDMKYDAIETIKVYATSPTAVPAIQVNECTAGFDVTGP"
     gene            complement(1386..3056)
                     /locus_tag="PLF85_10745"
     CDS             complement(1386..3056)
                     /locus_tag="PLF85_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE20188.1"
                     /translation="MNINPDPKTIQRPWLKHYDKHVPVSLSYPRESLPELFANSCAEN
                     AHRVALQFMGKSIRYGQVEASATAFAAYLTEQGINKGDRVFMMLPNSPHFVIAYYAIL
                     KLGAVAVPASPLDTPTEAAYKFSNSGAKLVIFLDLLYDKVSQLLQQKPNTPAVAADVS
                     EYLPFPKNLLFRLKKKSLGRPLPDFTRANLSTFAGVLRAYRKRAAETAQIAASDTAVI
                     IYTGGTTGVSKGVVLSHHAMVVNMTQARTWGDLSKDDVGLCVLPFFHGFGISIGLNLS
                     LSSGGKMVLMPRWDEAQAIRHFEKDSITVFAGVPTMYNAILNHKNFAKLKKAKLRGCF
                     VGAAPVPEALKVAFKEKTGGTLIEGYGLTEAVTAKCANPLYGLKKEKSIGIPWPDTLM
                     VIVDEKTGELLPPGKDGEIILHSPDLMSGYWQNPEATKATIKDGWLYTGDIGRMDEDG
                     YFSIVDRKKDLIITGGYNVYPAEIEDVLYQHPAVLEACVVGIRDDHLGEVGRAFVVAK
                     PGMAVTADELKSFLSDRLIKYKIPKQFVFRDSLPRSPVGKILRKELRG"
     gene            complement(3183..4916)
                     /locus_tag="PLF85_10750"
     CDS             complement(3183..4916)
                     /locus_tag="PLF85_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE20189.1"
                     /translation="MNGGEVAARVIAAHGVDALFTLIGGHVSPILVESKKRGIRVIDT
                     RHEVNAVFAADAWARLKGIPGVAVVTAGPGVTNTITAVRNALMAQSPLVLIGGATATA
                     LRGRGSLQDIDQIELIRSNVKRAFRVNQLRQIAPTLNRAFADAAEGVPGPVFVEIPVD
                     LLYDEKIVRDWYADASPKGKSLMSGIIRGYLKWHVAKLFRSAAQVKIPAPKENSPHFV
                     SDNLLAKLSAAMEKAERPLVLAGSQVLRDARMASDVRAALEKLGIPVYLSGMARGLLG
                     DRHPLQYRQKRKEALKECDLVILLGVPADFRLNYGAHIGRRAYQVNVNLDRKDLKKNK
                     RPNIAARVDSATLLLRLAEKGIFADAQKIAGWKAVLSEREKAREAEILKSASEKMKLI
                     NPVDFFLKAAPVLAKKTILIADGGDFVATGAYTLRPGGPLTWLDPGVFGTLGVGAGFA
                     LGAEVARPDHDVVILYGDGSAGYSIMEFDTFVRHKLPVGAIIGNDACWTQIYRDQVEF
                     LKDDVACMLDYSHYDEISKTFGGKGTTISKPAQVKNGLNALKNSLKKKQPFILNVLIG
                     RSEFRKGSISM"
     gene            5167..5643
                     /locus_tag="PLF85_10755"
     CDS             5167..5643
                     /locus_tag="PLF85_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNE20190.1"
                     /translation="MTKPDSVTVKNLEAAFAGESMAHMKYLHFARICREQGDEATAKV
                     FEETAAQEIQHALGHAELLFPREKLGVKEVLRYAIEGETYDYTEMYPKFRHAAVEEGN
                     SAAAAEFDEQIGESKDHAGLFKRTLEIAAKRFAALAKVEEKHAKRYEATLSAVGGK"
     gene            5674..5850
                     /locus_tag="PLF85_10760"
     CDS             5674..5850
                     /locus_tag="PLF85_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNE20191.1"
                     /translation="MRKFKCVVCGFVYDEAQGLPEDGIKPGTRWEDIPEDWECPDCGV
                     SKADFEMVELVGAK"
     gene            5946..>6188
                     /locus_tag="PLF85_10765"
     CDS             5946..>6188
                     /locus_tag="PLF85_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE20192.1"
                     /translation="MSNVVIIGSGMAGIGLAELLAQDSKFSVTVVSGETSGYYSRPLL
                     SHAFASEDVFGRTVLSDVASLQQKFRFIAGKTVAQIL"
BASE COUNT         1331 a         1719 c         1749 g         1389 t
ORIGIN      
        1 tacaagtggc cgatctttgc gcaggtaagc ctgcacggtg gttacggcag gcgcgactac
       61 aaccgcgacg gccaggctgt cccggtgcgg ctcattgcgg gtgagctggt accggcgctg
      121 ggttacagtt ttggcctacg ctggttcagg ctcgagccgt tcgcaggctt cggtctcggc
      181 tactccaacc tccagctcga ttattcgggc cttgcgcgca agccggtcga cacgactgcc
      241 gggctggcgc tttcgacgca gacgatcgac cagttctatt ttctttcgca ggccggtttc
      301 atcttcaagt acgcaccgca cccgcgctgg gcgattatcg tttcgccctc ggcgactgtc
      361 tttcattttg cgggcggtgc acaggctgaa ttgaatgccc gtatgggcgt ggggttttac
      421 ttctgatgca acaaagaata ctacaaggcg gaattatctg cgtcacggtg gtggtacttg
      481 cgtcgggttg catcgcgcaa aagccactcg acgtaacttt taccgagggc ggtaagggtg
      541 caggcatcgc ttcggggctc gtcgagaaaa cggcgacggc aaccgcaacg gtgagctcaa
      601 ttgccaactc tattggctca tcagttccta tactcgaatt cacagttaaa gagaacggtt
      661 caaaagatgg ttatcctcta caaatcagag agctacgatt caccaatatt gggactggcg
      721 atgcagcaga ttttcgcttc attcttgatg gtccgggcag caacaacacc atagggttct
      781 caaccggaca aaccgtagtc ttccgggatt ttggtgatat tataattcct gatggcgaga
      841 ctagcggcag aacgtaccag gtcaaagtgc acgtccgtga aaatatccag ggcagcacaa
      901 cggataacca gacagttcag ttgaagactt ctccaacgac agactttgac gtgaccgaga
      961 cttcctcgaa tcttttgtcg acaaacggcg catttcagca gacgtcagga actgtgatgc
     1021 aggttgttgc gacgcacctg cggggtggtg gcaccttcca gtttgccacc gtagcccgca
     1081 ataccgattt ccccggtaca cagaccattt atgctcagga tgttaaccag cgcgtagata
     1141 aagacttcac ctatgcaatt cagttagccc cccggacggg agcgctttgt gcctctgcag
     1201 ccgcaggtgc cctcagctcc accgacggcg gcctgaacaa ggcagcggtg cagggtgtgg
     1261 ctacatggac agacatgaag tacgatgcga ttgaaaccat caaggtctat gcgacatctc
     1321 ccactgccgt cccggcgatc caggtgaatg aatgcacggc cggctttgac gtcacggggc
     1381 catgatcacc ccctgagctc tttacgcaag atcttaccca ccgggcttct gggtaacgaa
     1441 tctctaaaca caaactgctt cggaatcttg tacttgatca ggcggtcaga caaaaagctc
     1501 ttcagctcat ctgcagtcac tgccataccg ggcttggcca ccacaaatgc gcggcccact
     1561 tcgccgaggt ggtcatctct gatgcccacc acgcaggcct cgagcactgc aggatgctgg
     1621 tacaggacat cttcaatctc tgcgggatac acgttgtagc cgccggtgat gatcaggtct
     1681 ttcttgcggt cgacgatcga gaagtaaccg tcttcgtcca tgcggccaat gtcaccagta
     1741 tagagccagc cgtctttgat ggttgctttc gttgcttcag ggttctgcca ataaccgctc
     1801 atgagatcgg ggctgtgcag aatgatctcg ccatctttcc ccggtggcag aagttcaccc
     1861 gtcttttcgt cgacgattac catcagcgtg tcaggccagg gaatcccgat cgacttttct
     1921 ttctttagtc catagagggg gttcgcgcat tttgctgtga cggcttcggt gaggccataa
     1981 ccttcgatca gcgtaccgcc ggttttttct ttaaatgcca ctttgagcgc ttcgggtacg
     2041 ggtgccgcgc ccacaaaaca accgcgcagc ttcgccttct ttagttttgc gaagttcttg
     2101 tggtttagaa tagcgttgta catcgtcggc acgccggcga aaactgtgat gctgtctttt
     2161 tcgaagtggc gtatcgcctg cgcttcgtcc caccgaggca tcagcaccat cttaccaccc
     2221 gaactcaggc tcaggttgag gccgatgctg ataccgaagc catggaaaaa cggcagtaca
     2281 cagagaccca catcgtcttt gctgaggtca ccccaggtgc gggcctgcgt catattcacc
     2341 accatcgcat ggtgcgatag cacaacaccc ttagagacgc ctgtcgtacc gccggtgtag
     2401 atgatcaccg cggtgtctga tgcggctatc tgcgcagtct ctgctgctcg tttgcggtat
     2461 gcgcgcagca cacccgcaaa ggtgctgaga ttcgcgcgtg tgaagtcagg caacggcctg
     2521 ccgagggatt tctttttgag acgaaagagc aggttcttgg gaaaaggcag gtactcgctc
     2581 acatcagccg ctaccgccgg ggtattgggt ttttgttgca acagttgtga caccttgtcg
     2641 tacagcagat cgagaaagat caccagcttt gctcccgagt tgctgaattt gtacgccgcc
     2701 tcggtggggg tgtcgagcgg gcttgccggt acagcgactg cgccaagctt tagaatggca
     2761 tagtaggcga tcacaaaatg cgggctgttg ggcagcatca tgaacacccg gtcgcctttg
     2821 ttgataccct gttcggtgag ataggcggca aacgcggtcg ccgatgcttc gacctggccg
     2881 tagcgaatag acttgcccat gaactgcagt gccacgcggt gcgcattttc agcgcagctg
     2941 ttggcgaaga gctcaggcag gctctcgcgc gggtagctga ggctaaccgg cacgtgcttg
     3001 tcgtagtgct ttagccaggg acgttgaatc gtctttgggt ctgggttaat attcatattc
     3061 ttctcccgtc ggtcacttcg actgcgctca gtgaccggat atattcgggt gagcatttcc
     3121 cggtcgttga gagcttctta gctctccgcc ttcggagcgc agtcgaaacg cccgattctg
     3181 ccttacatcg atatagatcc ttttctgaat tcgctgcggc ctatcagcac gttgagaatg
     3241 aagggctgct ttttcttcag gctgttcttc agcgcattca gaccgttctt tacctgtgcc
     3301 ggcttcgaga tcgtcgtccc cttaccgccg aaggttttcg agatctcgtc gtagtgcgaa
     3361 tagtcgagca tgcacgcgac gtcgtctttc agaaactcga cctggtcgcg gtagatctgc
     3421 gtccagcagg catcgttgcc aataattgcg ccaaccggca gcttgtgccg cacgaaggta
     3481 tcaaattcca tgatgctgta acccgccgag ccgtcgccgt agagaatgac gacgtcgtgg
     3541 tcgggccgtg ccacctcggc cccgagcgca aaacccgcac cgacacccaa agtgccgaac
     3601 acacccgggt caagccaggt gagcgggccg ccggggcgta gcgtataagc gccggtcgcg
     3661 acaaagtcac cgccatccgc aatgaggatt gtcttcttcg ccaataccgg cgccgctttg
     3721 aggaaaaaat ctaccgggtt gatcagcttc atcttctcag aagcggattt caatatctct
     3781 gcttcgcgcg ccttttcgcg ttcgctgagc acggccttcc agccggctat tttttgtgcg
     3841 tcggcaaaaa ttcccttctc agcgaggcgc agcagaagcg tcgctgagtc gacgcgcgcg
     3901 gcaatgttcg gccgcttgtt cttcttcagg tctttgcgat caaggttgac attgacctgg
     3961 tacgcacgcc tgccgatatg cgcgccgtag ttcagccgaa agtctgccgg cacaccgagc
     4021 agtatcacga ggtcacattc tttgagagcc tctttgcgtt tctgccggta ctgcagcggg
     4081 tgcctgtcgc ccaaaagccc gcgcgccatg cccgagaggt agaccggaat gccgagtttc
     4141 tcgagcgcag cgcgcacgtc gctcgccatg cgcgcgtcgc gcagtacctg gctgcccgcc
     4201 agcacgagtg gtcgctctgc cttctccatg gctgccgaga gtttcgcgag caggttgtca
     4261 gaaacaaagt gcggggagtt ttctttgggc gcgggaatct tcacctgcgc ggcgctgcga
     4321 aagagttttg ctacgtgcca tttgaggtaa ccgcggataa tccccgacat gaggctcttg
     4381 cctttgggcg aagcatcggc gtaccagtcg cgcacgatct tttcgtcata gagcagatcg
     4441 accggaattt cgacaaacac cggcccgggc acgccttctg ctgcgtcagc gaacgcgcgg
     4501 ttcagtgtcg gtgcgatttg ccgcagctgg ttcacgcgaa atgcgcgctt tacattcgag
     4561 cgaatgagtt cgatctggtc gatatcctgc agtgagccgc ggccacgcag tgcagtggct
     4621 gtggcgccac cgatgagcac gagtggcgac tgcgccatga gcgcgttgcg cacggcggtg
     4681 atggtgttgg taacccccgg gccggcagtc accacggcga caccgggtat acccttgagc
     4741 cgcgcccagg catcggctgc aaaaaccgca ttcacttcgt ggcgtgtatc gatgacgcgt
     4801 atgccgcgct tcttgctttc caccagaatc ggcgacacgt ggccgccaat cagggtgaag
     4861 agcgcatcga caccatgtgc ggcaatcacc cgtgccgcta cttctccgcc gttcatggtc
     4921 ggagaaaaat aaacggcgcc agattgtcaa tacaatagat aagaaggtct taatatcgta
     4981 atatacctgg cgactggttc gtccattacc tctcggcgcc gggtggggag acaggagcct
     5041 ctgcaaattc tgctcacaaa aattagacga tatctaaata ttatataaat tagaattgat
     5101 ctaaataata atcagacagg ggtcgatagg cggggatagc aaaaaccccc ttccacagga
     5161 ggaaagatga caaaaccaga ttcagtaaca gtgaaaaacc tcgaggctgc cttcgccggc
     5221 gaatcgatgg ctcacatgaa gtacctgcac ttcgcgcgca tctgccgcga gcagggtgac
     5281 gaggcgaccg cgaaggtgtt cgaagagacc gccgcgcaag agatacagca cgctctcggc
     5341 catgcggagc tgcttttccc acgtgaaaag ctcggcgtga aagaggtact ccgctatgcg
     5401 attgaaggag agacatacga ctacaccgag atgtacccga agtttcgcca cgcagcagtt
     5461 gaagagggca acagcgcggc agccgccgag ttcgacgaac agattggcga gtcgaaagac
     5521 catgcgggtc tcttcaaacg cacactcgaa attgccgcaa agcgtttcgc agcgctggcg
     5581 aaagtcgaag agaaacatgc aaagcgctac gaagcaactt tgagcgctgt cggtggaaaa
     5641 taacccgcat gcgttatctg aaaggaggat caaatgcgta agttcaaatg tgttgtctgc
     5701 ggcttcgtct atgacgaggc acagggtttg cccgaagacg gtatcaagcc cggcacccgc
     5761 tgggaagaca ttcccgaaga ctgggaatgc cccgactgcg gtgtgagcaa agctgacttt
     5821 gaaatggttg aattggttgg agctaagtag tgaggcgaga gcctcactaa gcacgcgcag
     5881 gcaaggggag cacccttcga caagctcagg gtgacaatag tccgaatata tcgaggagag
     5941 caaacatgag taacgtagtc atcatcggtt cgggaatggc aggcattggc ctggcggagc
     6001 ttctggcgca agacagcaaa ttttccgtca cggttgtctc gggtgagaca tcgggctatt
     6061 actcgcgccc gctcttgtcg catgcctttg cgtctgaaga cgtctttggg cgcacggtgc
     6121 tcagcgacgt cgcgagcctg cagcagaagt ttcgcttcat cgccggcaag actgtcgcgc
     6181 agattctt
//
LOCUS       DAOQJF010000196         6142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7240_length_6142_cov_4.793987, whole genome shotgun sequence.
ACCESSION   DAOQJF010000196 DAOQJF010000000
VERSION     DAOQJF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7240_length_6142_cov_4.793987"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1264
                     /locus_tag="PLF85_10770"
     CDS             14..1264
                     /locus_tag="PLF85_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE20193.1"
                     /translation="MIFIALRQLWARKRQTILTLLGIMLGAAAYIAISGILLGFREYL
                     LDQMVNNDSHVRISAKARLVEEKSFDHVFTRPNESLFWIVPPSGRKDSQKIDNVQEWV
                     SRLREDSDVEAYAPQLNVTVLYRKGKVTQNGRVMGIIPSMQTRVAQLEKYVLKGTLRE
                     LAENGQKIMLGEGLRENLGLRYGDSITVVNSRGDAQQFKVSGSFSIGNRQVDESLAYV
                     SLNTAQSIAGSPGQVSDIAVRLRDVRKSYPKAQQWSNDESAKVQSWEQINASMLDVFE
                     VQDMTRYMMSAIILIVAGFGIYNILSIVISQKKREIAILRSIGYESTDILTIFMTQGM
                     LLGFAGGVLGVLLGYVICWQLSHVTFSSPMTKTKQTTMMISFNYLIYVYGFLMALFST
                     TVASFLPALKAGKMSPIDIIRGQE"
     gene            1261..1980
                     /locus_tag="PLF85_10775"
     CDS             1261..1980
                     /locus_tag="PLF85_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE20194.1"
                     /translation="MSIRLENVVKSFGDPPIDIIKRVSFELKDGEFVALTGKSGSGKS
                     TLLYMVSSLDNPTAGKVLLQDQDIAQMDQQQLHRFRNQHMGFIFQYHYLLPEFSALEN
                     VLMPAIKAGVAEKKRDEAIEMLKRFELGHRMNNLPSQLSGGESQRVAIARALIMQPRF
                     IFADEPTGSLDSANAINVMQIFQEVNRTTGCTIVYVTHDAEFAAMAHKQILLVDGQIE
                     SIMANKTATGKAAPLRKKRLP"
     gene            complement(2035..2544)
                     /locus_tag="PLF85_10780"
     CDS             complement(2035..2544)
                     /locus_tag="PLF85_10780"
                     /inference="COORDINATES: protein motif:HMM:NF020909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HNE20195.1"
                     /translation="MNIYFDIIKQMAQSMRNLQAIMGKAEEHAARLKFEPDNYLGLRF
                     FPNMLPFSAQIRILCDISKFAAAYLAEKTPPVHEDNEKTWSDYKARLGLAIAHLETYK
                     AEDFANAADVKVSPKNFNGKYMTGHDYLICRQTPNFYFHLVTAYDLLREAGVEVGKTD
                     YLGPLPLRD"
     gene            complement(2641..3543)
                     /gene="argF"
                     /locus_tag="PLF85_10785"
     CDS             complement(2641..3543)
                     /gene="argF"
                     /locus_tag="PLF85_10785"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNE20196.1"
                     /translation="MSRSFLSLSSLSREEFSALLDRAIEGKKNPGTWGAPLAGKSVAL
                     FFEKPSTRTRVSFEVGVYQLGGQPIVLTQNDLQLSRGEDVADTARVLRRYVHAIMARV
                     KSHSTLEALASFSGIPVINGLSDKFHPCQALADYMTLRENNVPAGSRFVFCGDGSSNM
                     VHSLIIGSAHYGCEIVVATPAQLLPDAGVLTIAARLGAKVSITQDARAAARDADVLYT
                     DVWVSMGQEKEAELKKKLLGPYQLNEELLSLASKNAIVLHCLPAHKGEEITEGAFESA
                     NSKIFDQAENRLHAQKAVMEFLLK"
     gene            complement(3552..4253)
                     /locus_tag="PLF85_10790"
     CDS             complement(3552..4253)
                     /locus_tag="PLF85_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNE20197.1"
                     /translation="MKEFYVLNLDYRRIAIIAGGALVALGLTLFVGMAIGKGQGERQA
                     LKLAAEADAQKMGLVPEPQKHSAEHAEKVVVAQGPAAGPIAEKAETAPAGEVPLREDP
                     LVQGPPKKKIQKTSAAKAEAEESDAPRRVKRKSKKAIAKAEPEVSGKVDHNAGGRYTI
                     QVAAFKRAGEAQGLIAKLKEEGIKAQTEKSGAYYLVTVGRSKSKEKLSKALARLKELE
                     YDAYIRKIHKTSEET"
     gene            complement(4250..4894)
                     /gene="coaE"
                     /locus_tag="PLF85_10795"
     CDS             complement(4250..4894)
                     /gene="coaE"
                     /locus_tag="PLF85_10795"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNE20198.1"
                     /translation="MENPYAQVYGLTGVMGSGKSTVAELLRAEGAFVLDADEIARFVI
                     DPTSPWYTQLKAKLIAAFGVFSSSSLFAADGRLDRALLASVTFGHEDRVRLLNNIMHP
                     VIQREFATRLLRAEEGVPVVYDVPLLFETGLEKKLKATILVYAPEATCVARAVTRAAA
                     KGQPLTAEEARHRLRSQISIEKKRELADYIIDNSGDFEALKPQVAGLYQRLRGL"
     gene            complement(4940..5698)
                     /locus_tag="PLF85_10800"
     CDS             complement(4940..5698)
                     /locus_tag="PLF85_10800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE20199.1"
                     /translation="MGATGGLRTDMLYYQTIYPEYMAGELLDRFLAAGWYRIGQSFIT
                     TDLITHDEILIPVYWLRIRLASYRPSGSARRIRRRCAQLQTKILPFAITPEIEALYAR
                     YRESLSHTISPSVHEYLLDAEWNNAFDSRLIEVRDSGRLVAAGYFDLGARCSTGILHI
                     YEPGLSQFSLGKLLYLEAIDFSLGEGHHYYYPGYLSPGTEKFDYKLFADPDSTELFLR
                     PLHRWVPYNKVAHKLRRWGDRVTAVTRRYRLPHG"
     gene            5806..>6142
                     /locus_tag="PLF85_10805"
     CDS             5806..>6142
                     /locus_tag="PLF85_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE20200.1"
                     /translation="MSKARTFYDLFKLAAEAKPNLETFKVRRKSGRIEGRTFKEMHEL
                     ISALAAGLRAGGIQKGDRITLLCDGSPNWIIADAGIIAADAISVPRGTDVTDDDILYI
                     VGHSESRWAI"
BASE COUNT         1321 a         1731 c         1721 g         1369 t
ORIGIN      
        1 gtagaaactc ctcatgattt ttatcgcgct gcggcaactc tgggcaagaa aacgccagac
       61 catactcacg cttttgggca tcatgctcgg tgctgcagcg tacatcgcga tctcgggcat
      121 tttgcttggc tttcgcgaat acctgctcga ccagatggtg aacaacgatt cgcacgtgcg
      181 catttcggct aaggcgcgcc tggtcgaaga aaagagtttt gatcatgttt tcacgcggcc
      241 caatgagagc ctgttctgga ttgtgccgcc atcgggacgt aaggacagcc agaaaatcga
      301 caacgtgcag gagtgggttt caaggctgcg cgaagacagc gacgtcgagg cctatgcgcc
      361 tcagctcaat gtcaccgtac tctaccgcaa aggcaaggta acgcagaatg gccgggtgat
      421 gggtattata ccttcgatgc agacgcgcgt cgcgcagctg gaaaaatacg ttttgaaagg
      481 cactctgcgc gagctcgcgg aaaatggcca gaagatcatg ctcggcgagg gcttacgcga
      541 aaacctgggc ctgcgttacg gcgattcaat cacggtggtc aacagccgcg gcgacgcaca
      601 acaattcaaa gtgtcggggt cgttttcgat cggtaaccgg caggtagatg aaagccttgc
      661 ctacgtttcg ctcaataccg cgcagtcaat tgccggcagc cccggccagg tgtcagacat
      721 tgcggttcgc ctgcgggacg tccgaaaatc gtatcccaag gcacagcagt ggtcgaatga
      781 cgaatcagcg aaggtacaga gctgggagca gatcaacgcc tcgatgctcg acgttttcga
      841 agtgcaggac atgacgcgct acatgatgtc ggcgattatc ctcatcgtcg ccggttttgg
      901 catctataac attctgagta tcgtgatcag ccagaaaaaa cgggaaatcg ctattctccg
      961 ctcgatcggc tatgaaagta ccgatatcct cacgatcttc atgactcagg gcatgctgct
     1021 gggcttcgcc ggcggagtgc tcggcgttct gctgggttat gtcatctgct ggcagctctc
     1081 tcacgttacg ttctctagcc cgatgaccaa gaccaagcag acgaccatga tgatttcgtt
     1141 caattacctg atctacgtct acggctttct catggcgctt ttctcaacaa ccgtcgccag
     1201 ctttctgccc gccctgaagg caggcaagat gtcgccgatc gacatcatcc gggggcagga
     1261 atgagcattc gcctggaaaa cgtggtcaaa tcatttggcg atccccctat cgacatcatc
     1321 aagcgtgttt cgtttgaact taaggacggc gagtttgttg cccttacggg aaaatcaggg
     1381 tcgggtaaga gtaccctgct ctacatggtg agctcactgg ataaccctac tgcaggcaag
     1441 gttctgctgc aggaccagga tattgcgcag atggaccagc agcagctgca ccggtttcgc
     1501 aaccagcaca tggggttcat cttccagtac cactacctgt tgcccgagtt ctctgcactg
     1561 gaaaacgtcc tgatgccggc catcaaggcc ggtgtggcgg agaaaaaacg cgacgaggcc
     1621 atcgaaatgc tgaagcgctt tgaactcggc cacaggatga acaacctgcc gtcgcagctt
     1681 tcgggcggcg aatcacagcg cgtagcaatc gctcgcgcgc tcatcatgca gccgcgtttc
     1741 atttttgccg acgaaccgac gggctcactc gactcggcca acgctatcaa cgtgatgcag
     1801 attttccagg aggtgaaccg gacgaccggc tgcaccattg tctacgtgac acacgacgcc
     1861 gagtttgcgg cgatggcaca caagcagata ctgctggttg acggccagat cgaatcgatc
     1921 atggcaaaca agacagcaac cggcaaggcg gcgccgctca ggaaaaagcg gctgccttaa
     1981 ttggctttct agcaggttcc ggaacctgct tttggctcct ttttcaggag cctgctaatc
     2041 gcgcagcggc agggggccga ggtaatcggt cttgccgact tcaacgcctg cctcgcgcag
     2101 caggtcataa gccgtcacca ggtgaaagta aaagttcggt gtctgccggc aaatcaggta
     2161 atcgtgcccc gtcatgtact tgccgttgaa attcttgggg ctgaccttca catctgccgc
     2221 gttcgcgaaa tcttcggcct tgtacgtttc gaggtgggca atcgccaggc caagtcgcgc
     2281 cttgtagtct gaccaggtct tttcgttgtc ttcgtgcacg ggcggagttt tttctgccag
     2341 gtaggctgca gcgaatttgc tgatgtcaca caggatacgt atctgcgccg aaaacggcag
     2401 catgttcggg aaaaagcgca gaccgaggta gttatcgggt tcaaatttga gtcgggctgc
     2461 atgctcttct gctttgccca tgatcgcctg caggttgcgc atactctgcg ccatttgttt
     2521 aatgatgtcg aaatagatat tcattagtta agtatacaaa attctgcgca gctccgccat
     2581 atatgcgaag cgaagaatga gcgccgttgt tggggatttt ggcagtttca ctgccggtaa
     2641 ctacttcagc aggaattcca tcaccgcttt ctgcgcatgc aggcggttct cggcctggtc
     2701 gaagatcttt gaattcgctg attcaaaagc accttcggtg atctcttcac ccttgtgcgc
     2761 gggcaggcag tggagtacta tggcattttt gctggcaagg cttagcagtt cttcattgag
     2821 ctggtaaggg ccaagcagct tcttctttaa ctcagcctct ttttcctggc ccatcgagac
     2881 ccagacatcg gtatagagca cgtcggcgtc acgcgcagcg gcgcgggcgt cctgcgtgat
     2941 acttaccttt gcccccagac gcgccgcaat cgtcagcacc cctgcatcgg gcaaaagctg
     3001 cgccggtgtt gccacgacga tttcgcaacc gtagtgcgca gaaccgatga tcagcgaatg
     3061 gaccatgttt gacgaaccat cgccgcagaa cacgaagcgg ctgcctgcgg gaacattatt
     3121 ctcgcgcaat gtcatgtaat cagccagcgc ctggcacgga tggaatttat cggaaagacc
     3181 gttgatcacc gggataccgg aaaaactcgc caaggcctcg agagtgctgt ggctcttgac
     3241 ccgtgccatg atcgcgtgca cgtaacggcg cagtacgcgc gcagtatctg ccacgtcttc
     3301 gccgcgcgag agctgcaggt cattctgcgt cagcacgatc ggctgaccgc ccagctggta
     3361 gacccctact tcgaacgaaa cgcgcgtacg cgttgaaggt ttttcaaaga acagggcaac
     3421 ggattttccg gcgagcggag ctccccaggt acccggattc tttttccctt caattgcccg
     3481 atcgagcaat gcagaaaatt cttcacgcga caaggatgac agacttaaaa aactgcggct
     3541 catggctttc ctcaggtctc ttctgaggtc ttatggatct tgcgaatata ggcgtcgtac
     3601 tcgagttctt tgagccgtgc aagggctttg ctgagctttt cttttgattt gctgcgaccg
     3661 acggtgacga ggtaataggc tccgctcttt tcggtctgcg ccttgatgcc ctcttctttc
     3721 agtttggcga tgagtccctg ggcctcaccg gcgcgcttga atgccgctac ctgaatagta
     3781 taccgcccgc ccgcgttgtg atcaaccttg cccgaaacct caggctcggc tttggcgata
     3841 gcttttttgc tcttgcgctt cacgcggcgc ggggcatcgc tttcttctgc ctcagccttc
     3901 gccgcactgg ttttctggat ttttttcttt ggggggccct gcaccagggg gtcttccctg
     3961 aggggtactt cgccggcagg tgctgtttca gccttttctg ctatggggcc cgcagcaggg
     4021 ccctgcgcaa caacgacctt ttctgcatgt tcggcagaat gcttctgcgg ctcgggtacg
     4081 aggcccatct tctgagcgtc ggcttcggcc gcgagcttga gcgcctggcg ctccccctgg
     4141 cctttaccga tggccatacc gacaaaaagg gtcaggccga gcgcgaccaa agctccgccg
     4201 gcgatgatcg cgatgcggcg gtagtccaga ttcaagacgt aaaattcttt cataatcccc
     4261 tgagcctttg atagagcccg gcgacctgtg gcttgagggc ctcgaaatcg cctgagttat
     4321 caattatata gtcggcgagt tcgcgctttt tttcaatgga aatttggctc ctgagacggt
     4381 gccgggcctc ttcggctgtc agcggctggc ccttggccgc tgcccgggta accgcacgcg
     4441 ccacgcaggt cgcttcaggc gcatagacca gtatagtcgc cttgagcttc ttctcgagac
     4501 cggtttcaaa cagcagtggc acatcgtata ccaccggcac gccctcttct gcgcgcagaa
     4561 gccgcgtcgc aaactcacgc tggatcaccg ggtgcataat attgttcagc aggcgcacgc
     4621 ggtcttcgtg accaaaggtg acactcgcca gcagtgcccg gtcaagccgg ccgtcggcgg
     4681 caaaaagtga gctactgcta aaaactccaa atgctgcgat gagcttagct ttgagctggg
     4741 tgtaccaagg cgaagtggga tctataacga accgggcaat ttcatcggca tcgagcacaa
     4801 atgctccctc tgcacgcagc aattcagcga ctgtagactt gccgctaccc ataaccccgg
     4861 tgagaccata tacctgcgcg tagggatttt ccatcggcag gccagaacgg gaaccctgaa
     4921 cagaacaggt agcaaaagct taaccgtgcg gcagccggta cctgcgggtc acagcagtca
     4981 ccctatcacc ccagcggcgc aacttgtgcg ccactttgtt atacggtacc cagcggtgca
     5041 gcggccgaag gaaaagttca gtcgagtccg ggtcggcaaa gagtttataa tcgaactttt
     5101 ctgtgccggg actcaggtaa cccgggtagt agtaatggtg cccctcgccc agcgaaaaat
     5161 cgatcgcctc gaggtagagc aatttgccca ggctgaactg tgacagcccg ggctcataga
     5221 tatgcagtat gccagtgctg cagcgggcac cgaggtcaaa gtacccagcg gcgaccaacc
     5281 gaccgctgtc gcgtacctcg atcagccgtg aatcgaaggc attgttccat tcagcgtcga
     5341 gcaggtactc atgcaccgaa ggcgagatcg tgtgcgacaa cgattcgcgg tagcgcgcat
     5401 aaagcgcctc gatttccggc gtgatggcga aaggcagaat cttcgtctgc agttgggcgc
     5461 agcgcctgcg gattcgccgc gcagagcctg aaggccggta agaggcaagc cggatgcgca
     5521 accagtaaac cggtatcagg atctcatcgt gcgtgataag gtcagtggtg ataaaactct
     5581 gcccgatgcg gtaccagccg gccgcgagaa accggtcgag cagttcacct gccatgtatt
     5641 cagggtagat agtctggtag tacagcatat ccgtacgcaa cccgccggtc gctcccatgg
     5701 cgctcgcgta ccagtcagct tgtcaaaact ttccgggatc gccggagagt cgctgctggg
     5761 tgcatctgtt gacccgatga cagcggcatt tactttcacc cacctatgag caaagcgcgt
     5821 acgttttatg acctctttaa actggctgcc gaggccaaac ccaatcttga aaccttcaag
     5881 gtgcgccgca agagcggcag gatcgaaggc cgtaccttca aagaaatgca tgagctcatc
     5941 tcggcgctgg cggcaggcct gagggctggc ggcatccaga aaggtgaccg catcacgctg
     6001 ctctgcgatg gttcccccaa ctggattatt gccgacgcgg gcattatcgc ggccgatgcg
     6061 atcagcgtac cgcggggtac cgacgtgacc gacgacgaca ttctttatat cgtgggccac
     6121 agcgaaagcc gctgggccat cg
//
LOCUS       DAOQJF010000197         6073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7362_length_6073_cov_4.299435, whole genome shotgun sequence.
ACCESSION   DAOQJF010000197 DAOQJF010000000
VERSION     DAOQJF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7362_length_6073_cov_4.299435"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..814
                     /locus_tag="PLF85_10810"
     CDS             158..814
                     /locus_tag="PLF85_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20201.1"
                     /translation="MNTAALLIDSSTRHLLVGRVSKAGTALRCTETSAAGENAIDLAV
                     ADLFPDLAEISEICLGEGPGSFVGLRSAFAYTRMFAMLRAIPCRTFHSSRLWHLLLGV
                     PQDEWLLMRTNAKLYYAERFVPGRESAAVDIADAGNLTGKIYCYSGSWLAKNPQAENN
                     EIPAHWQKTDFAPEKIPGFQVNSALLRLSEVKPHDSLTPIYGHELHFKLAKGFNGQTN
                     "
     gene            798..3002
                     /locus_tag="PLF85_10815"
     CDS             798..3002
                     /locus_tag="PLF85_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNE20202.1"
                     /translation="MDKQTEKLIDKWIFHASKSGDLRLAGANPQKQEEDKKQKSKGKS
                     GRRKKAATNGNANAKAKTPASREDSEALRFLRSTGIAAEDNRGVRLAIEFTGALSSAK
                     SGVAFVPVGRGIEAVVPFAERGGALHRDKVSVTLTGFNRGRFTARVNRIVEPFSGEYF
                     ARILGAARAGGSASQYLLAELIDLPDRPQVLVAGKHKTGATLYLRRNEKRAQYLKEMR
                     GGSRPGGGDIHSGYSRTQAFVFEASNRKVREDRKGDLERLRLRYMLPEDFDKKLVPTK
                     KDLNALVKREMKNPDRVKVKGTYIFTIDGEDAKDFDDAIACRKIPGGFELDVHIADVS
                     FFVKPGTPLFDEALRRGNSYYLAGSVIPMLPEILSNEFCSLKPKTERLAFTARMRFDQ
                     KGKMTGYELFRSVIYIDKRYTYKEAHKALGKKKNPLADAMELAEVLIAHRDQQGRIDL
                     NIAEQKAIYNKEGKFLGLESQERLQSHRLVEECMLSANQAVANYALKYHLPILHRNHE
                     SMPIDKLERLNRYLEKYVARLKIMGTDQKEIARILGHESLDPVREVFQFLLLRSFMQA
                     NYMPESKGHWGLAFSEYAHFTSPIRRFADLVTHLQLGAHLRREKTTLKVADLEFYGRE
                     ASRLERIAFEAERADKKLLAVRALGHRVGESFSAWLSSFSQDKLFITLVDYPVEGEID
                     AMEVDRRGEIRMIDDFSIFAGKLQKTLSLGDRFKVTLVKADPLEMALKFTFS"
     gene            3110..3442
                     /locus_tag="PLF85_10820"
     CDS             3110..3442
                     /locus_tag="PLF85_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE20203.1"
                     /translation="MTEVEGVPCAVITAAQLHMYFIPDLKEKLLATVGGEPDNLIIDL
                     SQVEYLDSSAMGVLFQVQKKMQAYGGSLYLVGINATIEMVFKLTKSDQHFSVFTDAAA
                     AVKAIQEK"
     gene            complement(3489..3836)
                     /locus_tag="PLF85_10825"
     CDS             complement(3489..3836)
                     /locus_tag="PLF85_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20204.1"
                     /translation="MKKYRYPEAIREAVHLEYQHKAPESVRLRVKHLMQLPLVKPWQF
                     IGSVALLLASPLSFYLFGDKLVYSTTVLVMLNISAGVAAFMVIFAGVAHRYADPKHKA
                     ELLDKLHALRARI"
     gene            complement(3946..4461)
                     /locus_tag="PLF85_10830"
     CDS             complement(3946..4461)
                     /locus_tag="PLF85_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE20205.1"
                     /translation="MLNDEQGLAKEDFDREFERALDMIYSIGLKLYRGNEDDAADFAQ
                     SLYPFALRQSHKFSGRSAFSTWLYRVAWNFGLEDLRRKKRLVTETIDNYEIAASEPET
                     EFDEQEIANLRKHISGLPDGYRLPLIMLFYENMGYAEISSLTGVKEGTLKANVHRAKA
                     ILRQKLGKETA"
     gene            complement(4448..4843)
                     /locus_tag="PLF85_10835"
     CDS             complement(4448..4843)
                     /locus_tag="PLF85_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20206.1"
                     /translation="METNLKNLETMLERCVSTSHTADIILSIVPMVAVVFGSVLVFFL
                     MLYNYRLRKEKIRQQIPTDSWVEHLRFISLLAGCLSFSAGVPLTLFFVFTNPANPGIL
                     GGLIPLFAGIGLVAFYYLSERKNRIHAER"
     gene            complement(4925..5752)
                     /locus_tag="PLF85_10840"
     CDS             complement(4925..5752)
                     /locus_tag="PLF85_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20207.1"
                     /translation="MQQPGSPINVAISPCPNDTFAFCSLRHNPAYRLHYLDIEELNLG
                     LLEARFPISKGSFSLVDRIAGSYDLLPVGAAIGRGVGPVLVGRLFDGCRLALPGENTT
                     AHRLFHFYRKTVHPAMQLTQRQMPFFEILPALRRNEIDAAVLIHEGRFVYAREGLALI
                     EDLGDWYEKKTGAMIPLGAIYAHKDLPAKTVTAFTTALRQGIHNSRVAYNAKSSPYSL
                     EILPFMLEKAQEHETAVVEAHVDTYVTADTENLSAEAVKSIDVFRLHCLGQADQIPL"
     gene            complement(5752..>6073)
                     /locus_tag="PLF85_10845"
     CDS             complement(5752..>6073)
                     /locus_tag="PLF85_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20208.1"
                     /translation="KNTELALDAAEALTGRYGTTLTLDVWEFKSEADCRTAYDTLTAL
                     EKQNQPREYNQTRANESRYQFTRQTGIAGEIFTIKKVLFRILATDKNTTTEYLIASKL
                     ARAR"
BASE COUNT         1381 a         1646 c         1732 g         1314 t
ORIGIN      
        1 taatattttc tatccctcat aaataattgc aagcctggtt gtaggattat ccgccagaca
       61 gggcgccggc gcacctgttt gcccctttcg cgaagcagac agagctacgg cttgaagtga
      121 gtagcgacga gtcacgccat gtgacgtggc ggctgttatg aatactgccg cgctactgat
      181 cgacagttcg acgcgccacc tgctggtagg ccgcgtaagc aaagctggta ctgcgctgcg
      241 ctgcacggaa acatcggctg caggtgaaaa cgccatcgac cttgcagtag cagacttgtt
      301 tcccgatctc gccgaaatat cggaaatttg cctcggcgag gggccggggt cgtttgtcgg
      361 attgcgctcc gcatttgctt acacgcgcat gttcgcaatg ctgcgcgcga ttccgtgccg
      421 gacattccac tcgtcgcggc tctggcacct gctgctcggt gtaccgcagg atgaatggct
      481 gctgatgcgc acgaatgcaa agttgtatta cgccgagcgc tttgtgcccg gtcgcgaatc
      541 ggctgctgtg gatattgccg atgcaggcaa cctgaccggt aaaatctact gctattcagg
      601 cagttggctg gcgaaaaacc cgcaggccga aaataacgaa atacccgctc actggcagaa
      661 aacggatttc gcacccgaaa aaattcctgg attccaggtg aattctgccc tgttacggct
      721 ttcagaagta aagccgcatg actctctgac gcccatctac gggcatgagc tgcactttaa
      781 gctcgcaaaa ggatttaatg gacaaacaaa ctgaaaaact catcgataag tggatcttcc
      841 acgcatcgaa atcaggcgac ctgcgcctgg ccggggctaa cccgcaaaag caggaagaag
      901 ataagaagca gaagagcaag ggcaagagtg gccgcaggaa aaaagcagct accaacggta
      961 acgccaacgc aaaagccaaa actcccgcaa gccgcgaaga cagtgaagca ctgcgctttt
     1021 tgcgctctac gggtatagcc gctgaagaca accgcggggt acgtctcgcg attgaattca
     1081 ccggggcgct cagctcggca aaatcgggcg tggcgttcgt gccggtcggc cgcggcatcg
     1141 aagcggttgt accgtttgct gagcggggcg gggcgctgca ccgcgacaag gtgagcgtga
     1201 ccttgaccgg ctttaaccgg gggcggttta ccgcgcgcgt aaacagaatc gtcgaaccgt
     1261 tttcgggtga atatttcgcc cgcatactcg gggccgcgcg cgccggcggc agtgcctcgc
     1321 agtacctgct ggctgaactc atcgatctgc ccgaccgtcc gcaggtgttg gttgccggca
     1381 aacacaagac gggggcaaca ctctacctgc gccgcaacga aaaacgcgcg cagtacctga
     1441 aggaaatgcg cggtggctcg cgccccggcg gcggcgacat ccattcgggt tatagccgca
     1501 cgcaggcgtt tgttttcgag gcttcaaacc gcaaggtgcg cgaagaccgc aaaggtgacc
     1561 tggagcgcct gcgcctgcgc tacatgctgc ccgaagattt cgacaagaag ctggtgccga
     1621 cgaaaaagga cctcaatgca ctggtcaaac gcgagatgaa gaatcccgac cgggttaagg
     1681 ttaagggaac ttacatcttc accatcgatg gtgaagatgc aaaagacttc gatgatgcaa
     1741 ttgcgtgccg caagattccc ggtgggttcg aactcgacgt ccatattgct gacgtgagtt
     1801 ttttcgttaa gccaggaact ccgctctttg acgaagcgct gcggcgcggc aacagctact
     1861 acctcgcggg cagcgtcata ccgatgctcc cggagattct ctccaacgag ttttgcagcc
     1921 ttaagcccaa gaccgagcgc ctcgcattta ccgcgcgcat gcgcttcgac cagaaaggca
     1981 agatgaccgg gtacgagctc tttcgttcag tcatctacat cgataaaagg tatacttaca
     2041 aagaggcgca taaggcactg ggcaaaaaga agaacccgct cgccgacgcc atggagctgg
     2101 cagaggtact gattgcgcac cgcgaccagc aggggcgcat agatctcaat attgccgagc
     2161 aaaaggcgat ctataacaaa gaaggtaagt tcctgggcct cgaaagccag gagcgcctgc
     2221 agtcgcaccg ccttgttgag gaatgtatgc tgagcgctaa ccaggccgtg gccaactacg
     2281 cgctcaaata ccacctgccg attctgcacc gcaaccacga gagtatgccg atcgacaagc
     2341 tcgagcggtt gaaccgctac ctcgagaagt acgttgcacg cctgaagatc atgggtacag
     2401 accaaaaaga gattgcgcgt atcctggggc acgagagcct tgatccggta cgcgaggttt
     2461 tccagtttct gctgctgcgt tcgttcatgc aggccaacta catgcccgaa tcgaaaggcc
     2521 actggggtct tgctttcagc gagtatgcac atttcacttc gccaatccgc cggtttgccg
     2581 acctggtgac gcacctgcaa ctgggagctc acctgcgccg ggaaaaaaca accctcaagg
     2641 tggcagacct cgagttttac gggcgtgaag cctcaaggct tgagcgtatt gccttcgagg
     2701 ctgagcgcgc agacaagaag ctgctcgcgg tgcgcgcact gggacacagg gtaggagaat
     2761 catttagtgc ctggctcagc agtttcagcc aggataaact ttttatcacc ctggtcgatt
     2821 acccagtcga aggagaaatc gacgccatgg aagtcgatcg gcgcggtgaa atacggatga
     2881 ttgacgattt ttcaattttt gccggcaaac tacagaagac gctgtcactc ggcgatcgct
     2941 tcaaagtgac gctggtgaag gctgatccgc tcgagatggc gctcaaattc acatttagct
     3001 gacagcatag catgggcata aattgcccac ttgtgatcct ggctgatttg gctacacgac
     3061 aaagtattga cagcccgcgt gcctgtggag gtcgtccgct gtggtccgca tgaccgaagt
     3121 tgagggtgta ccctgcgcgg tgattaccgc tgcgcagctg cacatgtatt ttattcccga
     3181 cctcaaggaa aagctgctcg caacggttgg tggcgaaccc gataacctga tcattgacct
     3241 ctcgcaggtc gagtatcttg attcgtctgc catgggggtt ctcttccagg tgcagaagaa
     3301 gatgcaggcc tacggtggtt cgctctacct cgtcggtatc aacgccacga ttgaaatggt
     3361 tttcaagctt accaaatcag accagcactt ttcggttttc actgacgcag ccgcagccgt
     3421 taaagctatc caggaaaaat aaatccccgc ggcagcgccg ctgccgcaga ggttgggtag
     3481 tactgccctc agatacgggc gcgcagcgcg tgcagcttgt cgagcagctc ggccttgtgt
     3541 ttcgggtcgg cgtaacggtg tgccacgcct gcaaagataa ccatgaaagc cgccactcct
     3601 gcagagatat tgagcatcac cagtacggtg gttgagtaga cgagcttgtc gccaaagaga
     3661 tagaagctta aggggcttgc gagcagcaac gcgacgctgc cgataaactg ccatggctta
     3721 accagcggca gctgcatcag gtgcttcacg cgcaggcgca cagattcggg cgccttgtgt
     3781 tggtactcga ggtgaacggc ttcacgaatc gcttcaggat acctgtactt tttcatatcg
     3841 tctccttgtt acacgatgta acctggagac ggcggacacg atgtcctttc atgccgtctc
     3901 ctatgatgca ggacgtatct tggggacggc ggacacgatg tcctctcatg ccgtctcctt
     3961 gcccagtttc tggcgcagaa tcgcctttgc cctgtggacg ttggccttga gcgtaccttc
     4021 tttcacaccg gtgagggatg aaatttcggc gtagcccatg ttttcgtaaa agagcataat
     4081 cagtggcagg cggtaaccgt cgggcaggcc gctgatgtgc ttgcgcagat tcgcgatctc
     4141 ctgttcgtca aattccgttt caggctcgct cgcggcgatt tcgtagttgt cgatcgtctc
     4201 ggtgacaagc cgcttcttgc gccgcaggtc ttcaagcccg aagttccagg caacgcggta
     4261 aagccaggta gaaaaggccg aacggccaga gaacttgtgg ctctgtctga gcgcaaaagg
     4321 gtagagcgac tgtgcaaagt ctgcagcgtc gtcttcattg ccgcggtaca gcttcaggcc
     4381 aatcgaataa atcatgtcga gtgccctttc aaattcacgg tcgaaatcct cttttgccag
     4441 cccctgctca tcgttcagca tgaatccggt ttttccgttc agagagataa tagaatgcga
     4501 caaggccgat acccgcaaac agcggaatca gaccacccag tattcccggg tttgccggat
     4561 tcgtgaacac gaaaaacagg gtcaggggaa ctccggctga aaagctcagg cagcccgcaa
     4621 gcaggctgat aaaacgcagg tgctctaccc agctatcagt gggtatctgc tggcgtatct
     4681 tctctttgcg caaacgatag ttgtacagca tcagaaaaaa taccagcact gagccgaaga
     4741 cgaccgcaac catcggcaca atggacagaa taatatctgc cgtgtgcgac gtgctgacgc
     4801 agcgctcgag catggtttca agatttttca gattagtctc catacctgac cttcctttag
     4861 acggcctctc tggccgcagg gttgcaaaaa agcgggtggc atcgctgccg ggggcacagc
     4921 gtactcatag gggtatttga tcagcctgac cgaggcaatg tagcctgaaa acgtcaatcg
     4981 atttcacggc ttctgcggac agattttccg tgtcggcggt gacgtaggtg tcgacgtggg
     5041 cttcgaccac agccgtctca tgctcctgcg ctttctcgag catgaagggc agaatctcca
     5101 agctgtaggg ggagcttttt gcgttataag ccacgcggct gttgtggata ccctgccgta
     5161 aggctgttgt gaaagcagtc actgttttgg ccggcaaatc cttatgcgcg tatatggcgc
     5221 cgaggggaat catcgccccg gtcttttttt cataccagtc cccgaggtct tcgatgagcg
     5281 caaggccctc gcgagcgtag acaaaccgcc cctcgtggat gagcaccgct gcatctattt
     5341 catttcggcg caatgccggc aggatctcga agaacggcat ttggcgttgc gtgagttgca
     5401 tcgcaggatg cacagttttc cggtaaaaat ggaacagccg gtgtgccgtg gtattttcac
     5461 ccggcagcgc aaggcggcag ccatcaaaca gccgtcccac gagtacagga cccacaccgc
     5521 ggccaattgc tgcgccgact ggtaaaagat catagctgcc ggcaatacga tcaaccaatg
     5581 aaaacgatcc cttggagatg ggaaacctcg cttcgagcag gccgagattc agttcttcaa
     5641 tgtccaggta gtgcagtcgg taagctgggt tgtggcgcag actgcagaag gcaaaagtgt
     5701 cattggggca gggggagatg gcgacgttga tggggctgcc tggctgctgc atcagcgcgc
     5761 ccgggccagc ttgctcgcaa tcaggtattc ggtggtagta ttcttatcgg tggcgagaat
     5821 acgaaacagc accttcttga tggtaaagat ttcacccgcg atacccgtct gccgggtgaa
     5881 ctggtagcgg ctttcgttgg ctcgtgtctg gttgtattcg cggggctggt tttgtttctc
     5941 gagtgctgtt agtgtatcgt acgcggtgcg gcagtctgct tcacttttga actcccacac
     6001 gtcgagagtc agcgtcgtgc cgtagcggcc tgtcagcgct tcggcagcgt cgagagcaag
     6061 ctcggtattc ttt
//
LOCUS       DAOQJF010000198         6029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7431_length_6029_cov_6.206227, whole genome shotgun sequence.
ACCESSION   DAOQJF010000198 DAOQJF010000000
VERSION     DAOQJF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7431_length_6029_cov_6.206227"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLF85_10850"
     CDS             <1..186
                     /locus_tag="PLF85_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNE20209.1"
                     /translation="ILEEFGEKKKAPKESKDKTLEKVKFYSRTEVLQQMLNTALTKEN
                     YEEAARLRDELRTEINN"
     gene            complement(183..1223)
                     /locus_tag="PLF85_10855"
     CDS             complement(183..1223)
                     /locus_tag="PLF85_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNE20210.1"
                     /translation="MLSLFKKKEKPVYQAYIQSMDKTVTVKHRQTLLEAALEAGLDYP
                     YSCGAGSCTTCKTKLIQGQYKDLSDMSLVLTEDDLKKGYILACQALPKTDLIVTLEAD
                     ESKEYSGEVTGYRRLTHDIVEVKIRLSEKMRYSAGQYADLAVAGVKGSRSYSFAAAPG
                     AGGGSPEVYFHLRLVPNGEMTTWFHEKDRTGEKVKLRGPRGTFGWRDHKNTLLCIAGG
                     SGMAPIKAMLEDAAQKKEKRQIIYLFGARTQKDLYAVEEMEKLQKQLPNFRFIPVLSH
                     EEAGSGWKGARGLVTELIAQQQIDFADTAAFLCGPPGMIDAAIAALKEKGVTQNEIYF
                     DKFTDRSHIANR"
     gene            complement(1304..2209)
                     /locus_tag="PLF85_10860"
     CDS             complement(1304..2209)
                     /locus_tag="PLF85_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE20211.1"
                     /translation="MAARITLNLGGGAARGFAHIGAIRALVENDIVFDRIIGVSMGSI
                     CGAVYSFMPDVDFLEKRLKQLIHFEAFRDSVVGSWTANQDPAAKSIMRRAQKILTSTN
                     IMRRIITATGVLTAADVHAVLHPFLPDVQISATEIPFATAAVNIRTGELRVFQGTDRL
                     RSAVVASASMPLIFPPEKIDGEWYVDGGVLDKLGIETAARIGADRLIVVDVSDEKLPD
                     NLPRSGFDVMLKTEEIASEHRRSLQLARAKLVVRPIRGNYHWADYSAAEAFVQMGYEA
                     TQDKMSDIRAIATGKRVFGFLRRII"
     gene            2290..2832
                     /gene="lepB"
                     /locus_tag="PLF85_10865"
     CDS             2290..2832
                     /gene="lepB"
                     /locus_tag="PLF85_10865"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNE20212.1"
                     /translation="MIEKARHYRASGKGGTRKWKHYALIAAFVLSIALVLQFVFRHLF
                     FYPLKIATDSMQPEIATGDKRYFTYPRLSQPKPGDVVLVRPANADVQLLCRIIASDGD
                     KVLVREGRLYLNGKESRTLNYAVKSDADLAYNLPETEVRPNFVFCMNDNPRNTNDSRL
                     HGSFARQQIEAVLVKPTLFF"
     gene            2832..3758
                     /locus_tag="PLF85_10870"
     CDS             2832..3758
                     /locus_tag="PLF85_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE20213.1"
                     /translation="MPVTQPDKQAELAAKFRTRISPFVRRHAGTLPADDQLRFLKQFQ
                     PDPIELEDSTGFSDDPLREDDADIHPFADIVHKYSTKILYLTTDECPVYCRYCTRKRR
                     TLLSKGHDKTRLSDILDYLAQRPEVNEVIFSGGDPLMLPPGDLLERARQFLASPAIRY
                     LRFHTRAATTSPGLFSETFYVGLNELLRHHPQKLITFVLHINTAAEISEATRDIVRRL
                     HDMGIRCYAQTVLLKGINDSSPELAELCQALTQAMIQPYYLHQLDRVTGSAHFEVSDD
                     EARLIYGELKRLLPVYLLPKLVRDSKQGKYPL"
     gene            complement(3762..5375)
                     /locus_tag="PLF85_10875"
     CDS             complement(3762..5375)
                     /locus_tag="PLF85_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20214.1"
                     /translation="MKSMRSFCVICAVVMLLVTGRSAATKSNEVILGEKAYSRGAYDS
                     ARQHFEQAIENGDETGDPRLYIGLILESRRQYAESIPYFRAAAERPMQKKFKKVAYWK
                     LVILYRQAKLYPESLRYVERLEEMGEKSELFDRIRGEAEGYRGIAGGRDLKGYADIRK
                     ASALEKEFRERLANGADLAEVSDTAQAAIASYKLAISQDDRWKEYRWKIAGIHEKLKQ
                     PAEAQAVYRLIWEESGDTSAAYKLGFIARRNGQYRTALKYFGAALEKEIEDPQLKFYI
                     RYNAAQAHYGLGNFKDSYAHSRLARRLSGDLELSKKTTLGLKRIYCLGKLSQKEIDEE
                     YCQFSKKEENPLFLNLLEMKKAIAANQKEKAIRFATRIYETEATDQEEGSAKLPAYCM
                     SDLPVAIGVLFRGEKYRDVLELTDRFRKNLGEMPDYHGWRAVSHFALKEYGSASVEFD
                     KLEKPTPSQMNLHLMAMAHMGDFAGVKVKGTVYLRNDKAREKLLNNFRKMRLYEPLRQ
                     EPDFETWLNGGKGQPEASEPKDEDSSLIE"
     gene            complement(5393..>6029)
                     /locus_tag="PLF85_10880"
     CDS             complement(5393..>6029)
                     /locus_tag="PLF85_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE20215.1"
                     /translation="RIQEATRALEQLIAEAHLTNPDLKAVTEGMQARHHQLEMEKGGN
                     LPILFVAGRADMNYTNMRDYQQSTYAFDPFNRNIFAVVFGAKWDLDFGNTKAKIQKAE
                     AELEKMKAMQSEAETGIPLKIAMAMWEFEKQKIQMEIAQNKFKEANKWSLSEMTAYTA
                     GTGNAKDLIEALGAMLMTEKEMAESEFNLCIAAARLAMEIGDQSTLKSWQE"
BASE COUNT         1324 a         1745 c         1522 g         1438 t
ORIGIN      
        1 attctcgaag aattcggtga aaagaagaaa gctcccaaag agagcaagga caagactctc
       61 gaaaaagtga agttctattc gcgcactgaa gtgctgcagc agatgctcaa cacggcgctg
      121 accaaagaaa actacgaaga agcagcccgc ctgcgcgacg agctgcgcac cgaaattaat
      181 aactagcgat tggctatgtg gctgcggtcg gtgaacttgt cgaaatagat ttcgttctgc
      241 gttacgcctt tctctttcaa cgctgcaatc gccgcatcga tcattcccgg tgggccgcag
      301 agaaaagcag ccgtgtcggc aaaatcgatc tgctgctgtg cgatcagttc tgtaacgagg
      361 ccacgcgctc ccttccagcc tgaaccggcc tcttcatgag agagaaccgg tataaagcgg
      421 aaattgggca gctgtttctg cagcttctcc atctcttcaa ccgcgtagag gtctttctgg
      481 gtgcgggcac caaagaggta aatgatttgc cgcttctctt ttttctgcgc cgcgtcttcg
      541 agcatcgcct tgatcggagc cataccgcta ccgccggcaa tacacaatag tgtattcttg
      601 tggtcgcgcc agccaaatgt accgcgcggc ccgcgcagct tcaccttttc gcccgtgcga
      661 tctttttcat ggaaccaggt ggtcatctca ccgttgggta ccaggcgcaa gtgaaaatag
      721 acttcggggc tgccaccgcc tgcaccgggg gctgcggcaa aagagtagga gcgtgagcct
      781 ttgacgccag caactgcgag atcggcatac tgccccgcac tgtagcgcat cttttcagag
      841 aggcgaatct tcacctcgac gatgtcatgg gtcaggcggc ggtaaccggt cacctcgccg
      901 gagtattctt tcgattcgtc ggcttcgagc gtaacaatca gatctgtttt tggcaatgcc
      961 tggcaggcaa gaatgtaccc tttcttcagg tcgtcttcag tcagaacaag cgacatatct
     1021 gaaaggtctt tgtactgccc ctgtatgagc ttggtcttgc aggttgtgca actgccagcg
     1081 ccgcagctgt aggggtaatc aagcccggct tcgagagcgg cttcgagcag tgtctgccgg
     1141 tgcttcaccg tcactgtctt gtccatactc tgtatgtaag cctggtaaac gggtttttct
     1201 ttctttttga agagcgaaag catgtggctc gctgaaggat tgaaacgacc gggcaaacat
     1261 aattcgaccg cttatggccg cggctgcagc agtcgaattg gtttcagatg atacggcgca
     1321 ggaagccgaa tactcttttg cccgtcgcaa tcgcccgtat gtcggacatc ttgtcctgcg
     1381 tggcttcgta gcccatctgc acaaatgcct cggcagctga atagtccgcc cagtggtagt
     1441 taccgcgtat aggacgcaca accagctttg ccctggcaag ctgcaggctg cggcggtgct
     1501 ctgacgcgat ctcttcggtc ttcagcatca catcgaagcc actgcggggc aggttgtcgg
     1561 ggagcttctc gtcggacaca tccacgacga tcagacgatc tgcgccgatg cgtgccgcgg
     1621 tctctatgcc gagcttgtcg agaactccgc cgtcaacata ccattcgccg tcgattttct
     1681 ccggggggaa aattagcggc attgaggcag aggcaacgac agccgaacgg agacggtcag
     1741 tgccctggaa caccctgagc tcccccgtac gaatattaac cgcagcagtg gcaaacggga
     1801 tctctgtcgc actgatctgc acgtcgggta aaaacgggtg cagcaccgcg tgcacatcgg
     1861 cagcggttaa aacacctgtg gcggtgatga tgcgacgcat gatgttagtg ctggtcagga
     1921 tcttctgcgc acggcgcata atgctcttcg ccgctggatc ctggttggca gtccaggaac
     1981 ctacaaccga gtccctgaaa gcttcaaaat ggatgagctg cttcaggcgc ttctccagga
     2041 aatcgacatc gggcatgaag ctgtaaaccg caccacagat tgaccccatc gacacgccga
     2101 taatgcgatc aaaaacgata tcattttcga ccagcgcgcg aattgcgccg atatgcgcaa
     2161 acccgcgcgc tgcgccaccg ccgagattga gagtgatacg ggcagccatg gttcttattg
     2221 acgtagccgc tcagccaccg gctgtcgctg cagcttcgcc tgcgcaccag cggcaaagca
     2281 ggtaaccaca tgattgaaaa agccaggcac taccgcgctt ccggaaaagg aggcacgcgc
     2341 aaatggaaac actacgcact catcgcggca ttcgtacttt ccatcgcctt ggtactgcag
     2401 tttgttttca ggcatctctt cttttacccg ctcaagatcg cgaccgacag catgcaacca
     2461 gaaatagcca caggcgataa acgttatttc acctacccgc gcctctcgca gccgaaaccc
     2521 ggcgatgttg tactggtcag gccggcaaac gccgacgtgc agctactctg ccgcatcatc
     2581 gcgagcgacg gcgacaaggt tctggtacgc gaaggtcgcc tgtacctcaa cggcaaggaa
     2641 tcacgcaccc tgaattacgc agtcaaatca gatgccgacc tcgcctacaa cctgccggaa
     2701 actgaggtca gaccaaactt tgtgttctgt atgaacgata atccccgtaa cacgaatgac
     2761 tcgcgcctgc atggcagttt tgcgcggcag cagatagaag ccgtgcttgt gaaacccacg
     2821 ctcttcttct gatgccagta acacagccgg acaaacaggc agaacttgcg gcgaaatttc
     2881 gtacgcgcat ttcgcccttc gtgcggcgcc atgccgggac gttgcccgcg gatgaccagc
     2941 tgcgctttct gaaacagttt caacctgacc ctattgagct cgaagatagc accgggttct
     3001 cggatgatcc gctgcgcgaa gacgacgccg acatacaccc atttgccgat atcgtgcaca
     3061 agtattctac gaagatacta tacctgacta ccgacgagtg cccggtctat tgccgctact
     3121 gcacccgcaa gcgccgcaca ctgctttcca aggggcatga caagacgcgc ctctcagaca
     3181 tcctcgacta ccttgcgcaa cgtcctgagg ttaacgaagt gattttttcc ggtggcgatc
     3241 cactgatgct gccccccggc gacctgctcg agcgcgcgcg gcaatttctc gcatcacccg
     3301 caatccgcta cctgcgattt catacgcgtg cagcaaccac atcgccagga cttttttcgg
     3361 aaacttttta tgtcgggctc aatgagctgc tacgccacca cccgcagaag cttatcactt
     3421 ttgtcctgca catcaacacg gctgcagaga tcagcgaagc gacgcgcgac atcgtgcgtc
     3481 gcctccatga catgggtatt cggtgttatg cacagacggt gctgttgaaa ggtatcaacg
     3541 atagctcccc cgaattggca gagctgtgcc aggcactcac gcaggccatg atacagccgt
     3601 actacctgca ccagctcgac cgtgtcaccg gttcagcgca ttttgaagtc agcgatgacg
     3661 aggcgaggtt gatctacggt gaactcaagc gcctgctgcc ggtctacctg ctgccaaaac
     3721 ttgtgcgcga ttccaaacag ggcaagtacc cgctataaga attactcgat tagactgctg
     3781 tcttcgtctt tcggctcgct ggcctcgggc tgccctttgc cgccattcag ccatgtttca
     3841 aaatcgggct cctgccgcag cggttcatag agacgcattt tgcgaaaatt attcaggagt
     3901 ttctcgcgcg ccttgtcgtt gcgcaggtaa acagtacctt tcactttaac gccggcgaag
     3961 tcgcccatgt gcgccattgc catcaggtga aggttcatct gggagggtgt gggtttctca
     4021 agcttatcaa attcaacgct cgcggaacca tattctttca gcgcaaagtg agataccgcg
     4081 cgccagccgt ggtagtcagg catctcgccg agattcttgc ggaacctgtc ggtcagctca
     4141 agcacgtcgc ggtacttttc gccgcgaaac aacacgccaa ttgccacagg caggtctgac
     4201 atgcaatagg caggcaattt cgccgaacct tcttcctggt ccgttgcttc agtctcatag
     4261 atacgcgtag caaaacgaat ggctttctct ttctggttgg cagcaattgc cttcttcatc
     4321 tcaagcagat tcaagaacag gggattttct tcttttttgc tgaactggca gtattcttca
     4381 tcgatttctt tttgcgacaa tttaccgagg cagtagattc ttttcagtcc aagcgtggtc
     4441 ttcttgctca attccaggtc gcccgacaac cgacgcgcaa ggcgcgaatg tgcataagaa
     4501 tccttgaagt tgcccaagcc ataatgcgcc tgcgcagcgt tgtaacggat ataaaacttc
     4561 aactgcggat cttcaatttc cttctcgagc gccgcgccga agtatttcag cgcggtacgg
     4621 tattgaccgt tacgccgcgc tataaacccg agtttgtaag cggcgctggt gtcacccgac
     4681 tcttcccaaa taaggcggta gactgcctgt gcctcggcag gttgcttcag cttctcatgg
     4741 ataccggcaa ttttccagcg gtattctttc cagcgatcgt cctggctgat tgccagttta
     4801 tacgaggcaa tagcagcctg tgcagtatcg gaaacttctg cgagatcagc accgttcgcg
     4861 aggcgttcgc gaaattcttt ttcgagcgca gatgccttgc gtatatcggc ataacccttc
     4921 aggtcacggc cgccggcaat accccggtaa ccctctgctt cgccgcggat tctgtcaaag
     4981 agctcgctct tctcgcccat ctcttcgaga cgctcaacat atcgcagcga ttcgggataa
     5041 agttttgcct ggcgatagag tatcaccagt ttccagtacg caactttctt aaacttcttc
     5101 tgcattggcc gttcggccgc cgcgcgaaag tagggaatac tctctgcata ctggcggcgt
     5161 gactcgagta tcagaccgat atagagccgc ggatcgcctg tctcgtcacc gttttcaatc
     5221 gcctgttcaa aatgctgccg cgcagaatca tatgccccgc gcgagtatgc cttttcaccc
     5281 aggatcactt cgttgctctt cgtcgctgcc gaccgacccg tcaccagtag cataaccaca
     5341 gcgcagatca cacaaaacga tcgcattgac ttcattattt tccgtaggac cattattcct
     5401 gccacgattt cagcgtcgac tggtcaccaa tttccatcgc cagtcgtgca gcggcaatac
     5461 acagattaaa ttccgattct gccatttctt tctcggtcat cagcatagcg cccagggcct
     5521 caatcaggtc cttcgcattg cctgtaccgg cagtatacgc agtcatttcc gagagagacc
     5581 atttatttgc ctctttgaac ttattctgtg cgatctccat ctggattttc tgtttttcga
     5641 actcccacat tgccatcgca atttttagcg gaattcctgt ttccgcttcg ctttgcatcg
     5701 ccttcatctt ctcgagctcg gcttcggcct tctgtatctt ggccttggta ttgccgaaat
     5761 cgagatccca cttagcgcca aataccactg cgaaaatatt cctgttaaac gggtcaaacg
     5821 catacgtact ctgctggtaa tcgcgcatgt ttgtataatt catatcggcc cgacccgcaa
     5881 caaagagtat cggcaggttg ccgcccttct ccatctcgag ttggtggtgt cgggcctgca
     5941 taccttcggt tacagccttc aggtcagggt tagtcaggtg tgcttcggcg atcaattgct
     6001 cgagcgcgcg agtggcttcc tgaatacgt
//
LOCUS       DAOQJF010000199         5979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7527_length_5979_cov_5.469953, whole genome shotgun sequence.
ACCESSION   DAOQJF010000199 DAOQJF010000000
VERSION     DAOQJF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7527_length_5979_cov_5.469953"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PLF85_10885"
     CDS             complement(<1..1291)
                     /locus_tag="PLF85_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20216.1"
                     /translation="MRSFFKSLTIIALCLAGQLWAYSFTVHQNMPEDSVLYMEFQGTN
                     QQRWAADYLKAKAGGRYTGSCVVLGGESSGSSLNIQCGAHGINRVGGDKPDYVKDVFV
                     DHPILPFNWNAVGINVTSYTHFINLKTGDASGNRHVTNNHNFIDGYSYNTTYGFEEFG
                     SLDSLIALGLNASQFSIDPINCTHSACTEYLPVATGVNENPTVDYKQNGSTTPLGIAR
                     GDKRLGSDDGTNYNCLSDTAVIGNCPDEGAKLDSTFQVPNVQPGGGGDLDSFFVSNED
                     WVIWEPSYNAATFYYNEGWLEGFASRNNSLQPAAAVNRYYNISGDQILYYTLTHHYTG
                     DIVQMTHVWVSTGYNHTDIEGWAEEKYGKRLVGGSDADKNLENYLNTQAYMNVRQNRY
                     NLPVGNVHRLIIEQAFYTYLIRFRSGYDKMTTSNQGIW"
     gene            1495..2235
                     /locus_tag="PLF85_10890"
     CDS             1495..2235
                     /locus_tag="PLF85_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNE20217.1"
                     /translation="MENLLPLGAVALVFVLLSLKVINQYEQGLVFTFGKYTGMKHPGL
                     RFLVPIVQRMVRVDMRIRTLDVMKQQIMSKDNVPVHVNAAIFYKVSNPEQAIIEVQDY
                     QYAIAQYGQTALRDVIGGMNLDTLLTERQEIGDAIKKIVDKETHGWGLEVIGIKLQDI
                     ELPDDLKRIMSRQAAAEREKRANIIKSEGDKEAAKNLADAARMMDKAKGAMQLRTLQT
                     LDGLGPTSSNTVVMALPVEVLEWFKKGR"
     gene            2249..3967
                     /locus_tag="PLF85_10895"
     CDS             2249..3967
                     /locus_tag="PLF85_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE20218.1"
                     /translation="MKKAVFFVAICLLGPILGQMIITVDRTAPLDVQWAWALYRGDVD
                     WVTELIAKGSKVNKKLGPVTHHQSGTTLPGAGEYPIQVAVRGGGPDLIEYLAQKGANL
                     GVKNSEGQSLIHLAYDRPRALAALLKLKLNPAAQDKKGNTVLHLAIGDGVKLYEEQEA
                     VVRLLKENKRLLSLKNRAGLTVIQTAMLHHNRRAIEVLAPDRLAELDNKIAAAHAKVE
                     ADQKKKTEAYSGIDLSICKQPARMTASDVLIKAVNTNDRKLFDCVMKKKPVLANYRSC
                     VNEYAGCETKPHVVFEFNREELIDAMIDASSKEYLDADSHLGNTATALLFQSSGRASW
                     FKKLKAKGVRLNVANKYGRTPQALACEKSNDDVVKILGADSSGGFKVGEKVWIQGLSE
                     ATVVRACSHGAIVSDHGQKSFTQANLIRREPQAFLTQKTQQSAARENEIDPVAERKRD
                     KTKDIRAGAIVRVKSTSFKEKTSEPSMHAVCEAKTVLRRMPDGYYRGSIHCGGSAYYT
                     FNVGADIEEIKDKPPAEQSHNVRRCIERCLEKCGRPALGKEPSYGNTVCTQGCQPSCE
                     STWGSN"
     gene            4110..4763
                     /locus_tag="PLF85_10900"
     CDS             4110..4763
                     /locus_tag="PLF85_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20219.1"
                     /translation="MALTACGAEKPAIASVKESNCRQMPKSTPQQILEVFQCVANSGV
                     PSDLDEGTLVRLVKRAKSGNTLNQLLGQLLQPVPSGNAPLCKVILKWDSHVPRDTRGS
                     IALLEDMLNKKKDPTPENHVTVGDFFKAVMLTRNISEKNVRGVTACYNLRYMATADAI
                     GEHIQHDYGFLFLAAQTDKFTLPLATVIVGREVLAVGGFKPDHMQRLKSFRRMAGLE"
     gene            4938..5942
                     /locus_tag="PLF85_10905"
     CDS             4938..5942
                     /locus_tag="PLF85_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE20220.1"
                     /translation="MTPSLFAATEEALRAAAEAADPVALEAALKKKPNVNAPLDEYGQ
                     TALFLVIGQESKGDDRLLKCTKLLISAGADVNQRAFSDQNTPLLSLISPYSDRTDEHR
                     AIIEVLAAAGADLNASSKDGSTAMSRAIQRVDLDTMKKLIALGAEMNPVLPEGRNYVH
                     LAARLDMDKRGVDKALEILINAGVDFDTPDERGRTPLHYIAESGNEAALKQILKYKPR
                     IDRKDTEGKTPVDLALRKKGIAETDGYERQLAAMIRRSAKVPRHVEVGEVFAANGAQV
                     DIMGKGVAKIKLGEKLLVKTAQGDYTIIAGENMHTKLKAKASPAAAARLKKGDKVYRK
                     "
BASE COUNT         1482 a         1500 c         1699 g         1298 t
ORIGIN      
        1 gccagatacc ctggttcgag gtagtcatct tgtcgtagcc cgaacggaac cggataagat
       61 aggtgtagaa ggcctgctca atgatcaacc ggtgcacatt gcccactggc aggttgtagc
      121 ggttctgccg tacgttcatg tatgcctgcg tgttcaggta gttttccagg ttcttgtcgg
      181 catcgctgcc gcccaccagg cgcttgccgt atttctcttc tgcccagccc tcgatgtcgg
      241 tgtggttata cccggttgaa acccagacgt gcgtcatctg gacgatatca cccgtatagt
      301 ggtgcgtcag cgtgtagtag agaatctggt cgcccgaaat attgtaatag cggttcactg
      361 cagctgccgg ttgcaggctg ttgttgcggc tcgcaaaacc ttcgagccag ccttcgttgt
      421 agtagaaagt cgcagcgttg taagagggct cccagattac ccagtcttcg ttgctgacaa
      481 aaaagctgtc gaggtcaccg ccgccacctg gttgtacgtt gggtacctgg aatgtactgt
      541 cgagttttgc gccttcgtca gggcagttgc cgatcaccgc ggtatcgctg agacagttgt
      601 agttagtacc gtcatcagag cccaggcgct tgtcgccccg cgcaattccg agcggggtcg
      661 ttgaaccgtt ctgcttgtaa tcaaccgtcg ggttttcatt cacaccggtc gctacgggca
      721 aatactccgt gcaggccgag tgcgtgcagt tgatcgggtc gatagaaaac tgcgatgcgt
      781 tcagaccgag cgcgataaga gaatcgaggc tgccgaattc ttcaaagccg taggtcgtgt
      841 tgtatgaata accatcaatg aagttgtggt tgttcgtaac gtgacgattg ccactggcgt
      901 cgcctgtttt gaggttaata aagtgcgtgt agctggtcac gttgataccc accgcgttcc
      961 agttaaaagg aaggatgggg tgatcgacga agacgtcttt gacgtagtca ggcttgtcgc
     1021 cgccgacccg gttgatgccg tgcgcaccgc actggatatt tagggaagat ccggaagatt
     1081 caccgcccag caccacgcaa ctacccgtgt agcgcccgcc tgcctttgcc ttcaggtagt
     1141 cggcggccca gcgctgctgg ttcgtgccct ggaattccat gtagagcacc gaatcttcag
     1201 gcatgttctg gtgcacggtg aagctgtacg cccacagttg cccggcaaga cagagggcta
     1261 taatcgtcag agatttaaag aaagagcgca tagttcgacc ttatgggtaa taactttcct
     1321 gatggtttat gccgccccag cagtgcgccg ggccatttat ttggagaaca agtgttttct
     1381 tcagttggta cgaagagaca taataatttc tgcgtcggag cgataggggt ttcgtggctt
     1441 tcggtcgaat accgaagcac ttgacgtgca gcaaacctaa gataactccc acctatggaa
     1501 aatcttctgc ctttgggtgc agtcgcgctc gtctttgtgt tgctcagcct caaggtgatc
     1561 aaccagtacg aacaggggct cgttttcact ttcggaaaat acacgggcat gaagcacccg
     1621 ggtctgcgtt ttctcgtacc gattgttcag cgcatggtgc gcgtcgatat gcgcatacgc
     1681 acgctcgatg tcatgaagca gcaaatcatg agcaaagaca acgtgcccgt gcatgtcaac
     1741 gcggcgattt tctacaaggt ttcaaacccc gagcaggcaa ttatcgaggt gcaggactac
     1801 cagtacgcga ttgcgcagta cgggcagacg gcgctgcgcg acgtcatcgg gggaatgaac
     1861 ctcgatacgc tgctgaccga gcggcaggaa atcggcgacg ctatcaagaa gatcgtcgat
     1921 aaagagacgc atggctgggg actcgaagtg atcggcatta aactgcagga tatcgaactg
     1981 cccgatgatc tcaagcgcat catgagtcgg caggcagcgg cggagcgcga gaagcgggcg
     2041 aacatcatca agagcgaggg cgacaaagaa gccgcgaaga acctcgccga cgccgcgcgc
     2101 atgatggaca aagccaaggg cgccatgcag ttgcgcacgc tgcagacgct cgacggcctg
     2161 gggccgactt cctcgaacac ggtggtcatg gctctgccgg ttgaggtgtt ggagtggttt
     2221 aagaagggaa ggtagtttgg gctttgccat gaagaaagcg gttttcttcg tcgccatctg
     2281 cctgttgggc ccgattcttg ggcagatgat aatcacagtc gatcgcacag caccgctcga
     2341 cgtacaatgg gcgtgggctc tctaccgagg cgacgtcgat tgggtaacgg agctcattgc
     2401 gaaaggttcc aaagtgaaca agaagttggg tccggtgacg caccaccagt ctggtaccac
     2461 attgcccggt gccggcgagt atcccattca agtcgcggtc cgcggcggcg ggcccgatct
     2521 aatcgaatat ctcgcgcaaa aaggcgcgaa tcttggtgtt aagaattccg aagggcaaag
     2581 tctgatccat ctcgcctatg atcgcccgcg ggcattggct gcgcttttga agcttaaact
     2641 caatcccgct gcgcaagata aaaaaggcaa tacggtactt cacctcgcta ttggtgacgg
     2701 tgttaaactc tatgaggagc aagaagcagt tgtgcgattg ctcaaagaga ataagcgact
     2761 gctctcgctg aagaataggg caggtctaac ggtcatccaa acggctatgc tgcaccataa
     2821 ccggcgtgcg attgaggttt tggcacctga tcgtctcgca gagctcgaca ataagattgc
     2881 agccgcccac gccaaggtcg aggccgatca gaaaaaaaag actgaagcat attcagggat
     2941 tgacctgagc atctgtaaac aaccagcgcg gatgactgca tcagacgtat taataaaggc
     3001 agtcaacaca aatgaccgaa agctttttga ttgcgtaatg aaaaagaagc ctgtattggc
     3061 gaactaccga agctgcgtca atgagtatgc aggctgtgaa accaaaccac atgttgtttt
     3121 tgaattcaac cgcgaagaac tgatagatgc catgatcgac gcatcgagca aagaatacct
     3181 cgacgcagat tcgcacctgg gcaacacggc gacggcattg ctctttcaat catcaggcag
     3241 ggcgtcgtgg tttaagaagc ttaaagcaaa gggcgttcgt ctcaatgtcg ccaacaaata
     3301 cggcagaact ccgcaggcac ttgcgtgtga aaaaagcaat gatgacgtgg tgaagatcct
     3361 tggcgcagac agctctgggg gtttcaaagt cggagaaaaa gtttggattc aggggctcag
     3421 cgaagctacg gtcgtgcggg cctgctcgca tggggccatt gtatctgatc atgggcagaa
     3481 gagttttacg caggcgaatt tgattcgccg ggaaccccag gcgttcttga cgcaaaagac
     3541 acagcagtct gcagcgcgcg aaaacgagat cgatcccgtt gcagagcgta agcgagacaa
     3601 aaccaaagat ataagggccg gcgcaatcgt tcgcgtcaag agcacgagct tcaaagaaaa
     3661 aacaagcgaa ccgagcatgc acgcggtatg tgaagcaaag acagttctca ggcgaatgcc
     3721 cgacggctac tatcggggta gcatccactg cggcggcagc gcctactata cgttcaacgt
     3781 gggcgccgat attgaagaga tcaaggacaa gccaccagca gaacagtcac acaatgtccg
     3841 gcggtgcatc gagcgctgcc tggagaaatg cggcagaccc gcactgggca aggagccttc
     3901 atatgggaat accgtttgta cccagggatg ccagccttcg tgtgaaagca cgtggggtag
     3961 caattgatca tctagactgg aattaatgcg gaggctgcaa ttatgaaaaa tcctgttctt
     4021 atggcgatgc agatttttaa tctggtgcgt gggcaacctt gttattgaat gctttggttg
     4081 tgctgagagc ctcgcttgcg atttgcgctg tggctctgac cgcttgcggt gccgagaagc
     4141 cggcaattgc ttctgtcaaa gaaagtaatt gccggcaaat gcccaaatca accccgcagc
     4201 agattcttga agtattccaa tgtgtggcca attccggcgt accgagcgac ttagatgaag
     4261 gcacgctcgt caggcttgta aagcgagcaa aatctggtaa taccctgaac cagttgcttg
     4321 gtcagttgct gcagcccgta ccctcgggaa atgcaccgct atgcaaggtg atattgaaat
     4381 gggattcaca tgtgccccgt gatacgcgtg gttcgattgc actcttggaa gatatgctca
     4441 ataagaagaa agatccgaca cccgaaaatc acgtaacagt gggcgacttc ttcaaagctg
     4501 ttatgctcac cagaaacatc tcagaaaaaa acgtgcgtgg tgtaacagcc tgctacaatc
     4561 tcaggtatat ggcgacggct gatgccatcg gggagcatat ccagcatgat tacggttttc
     4621 tattcttggc agcacaaacc gacaagttca cattaccgct ggcaacggtt atcgtcggca
     4681 gagaggtatt ggcggttggc ggcttcaagc ccgaccatat gcagcgatta aaaagttttc
     4741 gccgcatggc aggcctggaa tagaaaagag gcttgtccca agcaccggga ccacaattag
     4801 ccggcgtttt ggcattgcgg cctctgcatt ctatttgcct tgggctggcg taaaataagt
     4861 tgtcgcctat tcagaaaatg ctgctacggt gcatgagagc aagtttgctt cactgtgtct
     4921 attggatggc cgtcttggtg acgccgtcac tcttcgccgc caccgaagag gcgctgcgtg
     4981 ctgcggccga agcggcagat cccgttgccc tcgaagctgc attaaagaag aagcccaatg
     5041 tcaatgcgcc gctcgatgag tacggacaaa cggcactctt tctggtcatc ggtcaggaat
     5101 ccaaaggtga tgatcgcctt ttgaaatgca caaagctgct gatcagcgca ggagccgatg
     5161 tcaaccagcg tgcgtttagc gaccagaaca cgccgttgtt gagcctcatc tctccgtaca
     5221 gtgatcggac cgatgagcac cgggcaatta tcgaagtact ggcagctgcc ggtgcagatc
     5281 tgaacgcaag ctcaaaagac ggtagtacag cgatgagtcg cgcgatccag cgagtcgatc
     5341 tcgatacaat gaaaaagctc atagcgcttg gcgccgaaat gaatccggtc ttacccgagg
     5401 gacgtaacta tgtacacctg gcggcccgcc tcgatatgga taagcgaggt gtcgacaaag
     5461 ccctggaaat tctcatcaac gctggtgtgg atttcgatac gcccgacgag cgcggcagaa
     5521 ctcccttgca ctacattgcc gaatcgggca atgaagcggc attaaaacag atactcaagt
     5581 acaagccgcg catcgatcgt aaggacactg agggcaagac accggttgat cttgctctgc
     5641 gcaaaaaggg gattgcagag actgacggtt acgaacgcca gttggcggca atgatccgcc
     5701 ggtccgcaaa ggtgccgcgg catgtcgagg tcggtgaagt ttttgcagcc aatggagccc
     5761 aggtggatat catgggcaag ggcgtggcga agatcaagct gggtgaaaag ttactggtca
     5821 agaccgcaca gggcgattac acgattatcg ctggcgaaaa tatgcacact aaactgaaag
     5881 caaaggcttc accggcagct gcagcgcgtc tcaagaaggg agacaaggtc taccggaagt
     5941 aggctgccgc aacctgccca aacccccgtt gccatggct
//
LOCUS       DAOQJF010000200         5974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7541_length_5974_cov_5.303599, whole genome shotgun sequence.
ACCESSION   DAOQJF010000200 DAOQJF010000000
VERSION     DAOQJF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7541_length_5974_cov_5.303599"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="hisD"
                     /locus_tag="PLF85_10910"
     CDS             complement(<1..837)
                     /gene="hisD"
                     /locus_tag="PLF85_10910"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNE20221.1"
                     /translation="MSRLLRTDRYNDLNPTELQQLFARSGLDLSQVREQVIQPLARQF
                     RENAAQALQQAIQKFEGTLPEQLVLGPNELEAAYERVIETQKEVIDAFELAATNIRQF
                     HEAQKQRGFSSEIAGNTLGVNFVPFDRVALYVPGGKALYPSTVLMGVLPARIAGVRDI
                     TLLTPPVPETRRVADVVLAMAHIAGATRVLQAGGAQAIFAAAYGIDAIGLNPVDFIYG
                     PGNIYVSAAKIHVAGENLCGIDSFAGPSEVVIIADDSANARYVAHDLMAQAEHDENAQ
                     AIL"
     gene            complement(868..1149)
                     /locus_tag="PLF85_10915"
     CDS             complement(868..1149)
                     /locus_tag="PLF85_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20222.1"
                     /translation="MLIAMQRDGSFVHRNEFMHPESFSVLDDALTFCGKDMQEKLQSG
                     SYFRSQIYGFDLVRYRNLASQNMYIAYRVAIHNVNKALIDKDFESLTYI"
     gene            complement(1261..1698)
                     /gene="gspG"
                     /locus_tag="PLF85_10920"
     CDS             complement(1261..1698)
                     /gene="gspG"
                     /locus_tag="PLF85_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNE20223.1"
                     /translation="MRIKALRKRKGFTLIELMVVLVILGGLMGVLLVVLKDDKASRAK
                     AEVELKVTYGKIQFALFEFKNKFGRVPTSEEGLDALVTQPPGLEGWSGPYMDKRYLLD
                     PWKNRFKYNVEGSQAKITSLGADGREGGEKDDADIDLSTILDR"
     gene            1834..3204
                     /locus_tag="PLF85_10925"
     CDS             1834..3204
                     /locus_tag="PLF85_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20224.1"
                     /translation="MRKAFGSLMALIISALLISSIISFFTRDSFDTLPVKASNAPVKL
                     TRRMQETSFMVSAEYVSRYDFNWLAEKLQLPKEITHRILPVPANFYISNQLAHAEYPF
                     AGLEVVDAECNVKAERIPALRRYFKSLQVYTSEAKKTPLHIASFRSMPEGITLRYTDN
                     SARAAKCLAAQVVALGEISFLQDSVSLVLPAYQSVRVNLAPYERTALLAQAGRNIYNA
                     LAEAHKKNPAHRVRVLLTNMVPYGHAQVLPFSELAGQFVGLTPEELTAIRNAAVAANG
                     SVVFVPDLAKRIELGLDTSVIWQLWQTAKLPFYKDASGEPLLYEDMVLALRRSLTETD
                     GAGVVQNRRLKAIYASYARDNDEKRKWGESINLPYQRASTERLQKLQVQMLLDVYDFS
                     AVGLEAETYIALEMPARERFPLLYMTVRPLAKKLGFDEKKDGSDSAEIEGSLQLLRSA
                     LAQAGK"
     gene            3208..3582
                     /locus_tag="PLF85_10930"
     CDS             3208..3582
                     /locus_tag="PLF85_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20225.1"
                     /translation="MLHPYTALVWGMGASLLCFYAAFFYRKRNNLRHRQLAGLGILLN
                     LISSVYLIYAVRAVGVEMPSEYSLPVITAHRIFATLMALAMLAMAASGIRRRVEFHRR
                     LSWFFLPGYTLTYFSGMVIFHG"
     gene            3607..4476
                     /locus_tag="PLF85_10935"
     CDS             3607..4476
                     /locus_tag="PLF85_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNE20226.1"
                     /translation="MDFIVGNEAEKKQLEAICSKQAADVTESDLKFIARRIRQNAVEM
                     VFRANSGHPGGPLGLADIYAYLYFKILNLKGEHRDRLLISNGHVCAVRYSAMAVRGQL
                     PESELATFRRLGSRLQGHPSTKYLPEVENSSGSLGQGLANASGLALGLRLQKSPARVF
                     LGMSDGECQEGMTWEAAMAAAHNKLTNIHPFLDFNNIQIDGKVEKVMGVEPLDKKFET
                     FGWKVRVASGHDFGEIAAAFDWSLKAEGAPKMILFRTVLGKGVSFMENNPDWHGTPPN
                     QKDRDAAMIELSA"
     gene            complement(4497..5378)
                     /locus_tag="PLF85_10940"
     CDS             complement(4497..5378)
                     /locus_tag="PLF85_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNE20227.1"
                     /translation="MSPLKRISEHAFPRFFSAFLSLFILTALFLELDLRKLIFGDSES
                     EKGQNTEIIRALLENAQPVQAKKRKTVKFLSDKESEAQGQLTKEKGFESISTDYVLSP
                     GEMQPMQGRIVSGKKLAKILTAKESRVTIAAPQEQTSQSASAERLRIPAYYRFRHDFA
                     LSWDYLGRPAIPTARFAHYSYFRSMISKIQSVWAPPGGDPYPTFGDSYHRMNYAAGYT
                     RFSTFPSQDIYIHFLLDENGVVKDAKLVSSLGYQSLDRSCIEALYAAHDFGPPPKELL
                     DGNGVLIVPFIFRIIVR"
     gene            complement(5353..>5974)
                     /locus_tag="PLF85_10945"
     CDS             complement(5353..>5974)
                     /locus_tag="PLF85_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20228.1"
                     /translation="AQEPLALYGKGMLLADEQITQEIALAMLRQATQKSSLPEKYRVR
                     GFLRVAEIFADRKDKEEALQNLARITAAQRLADGQNVRRQAEIYLKLKEKDRAREVLT
                     AYLEGHGSDETTEYFLLKLYVVTMKDLKAAGKLCQRVAWQKSQNNKYLFNCARVSAAL
                     NDYTGALALTDLYIRRSGETVPKEASELREAIGRKRGKFEPAEADF"
BASE COUNT         1284 a         1641 c         1652 g         1397 t
ORIGIN      
        1 cagaattgcc tgcgcattct cgtcgtgttc ggcctgcgcc atcagatcgt gcgccacgta
       61 acgcgcattg gctgaatcgt cggcgatgat caccacttcg ctggggccgg caaacgagtc
      121 gatgccgcac aggttttcac ccgccacgtg gatcttggcc gccgaaacgt agatgttgcc
      181 gggcccgtag atgaagtcga ccgggttgag accaattgcg tcaataccgt acgcagcggc
      241 aaagatcgcc tgcgcgccgc cggcctgcag gacacgggtt gctccggcga tgtgcgccat
      301 cgccagcacc acatcggcaa cacggcgcgt ctcgggcact ggcggggtaa gcagggtaat
      361 gtcccgtacg cctgcaatac gcgccggtaa aacgcccatg agaacagtgg aagggtagag
      421 tgctttgccg ccaggcacat acagagccac gcggtcaaaa gggacaaaat tcacgcccag
      481 cgtgttgccg gcgatttcag aggaaaatcc cctttgtttt tgtgcttcgt ggaactgccg
      541 gatattggtg gctgcaagct cgaatgcatc gatcacttct ttttgcgtct cgatgacacg
      601 ctcgtatgcc gcttcgagct cattcggccc tagcacgagc tgttcgggca acgtaccctc
      661 gaacttctga atggcctgtt gaagcgcctg tgcggcattc tcgcggaatt gccgtgccaa
      721 cggctggata acctgttcac gcacctgcga caggtcaagc ccgctgcggg caaacagctg
      781 ctgcaattcg gtgggattta aatcgttgta gcggtctgtt ctgagcaggc gggacacccc
      841 gccagttacg gcaacttttt cagcgggtca aatatatgtc agggattcga aatctttatc
      901 gatgagtgcc ttgttgacgt tgtggatggc cactcggtag gcaatgtaca tgttctggct
      961 cgcgaggtta cggtagcgca ccaggtcgaa tccatagatc tgcgaacgga agtagctgcc
     1021 agactgcagt ttttcctgca tgtctttacc gcaaaaggtc agtgcgtcat cgagcaccga
     1081 aaaactttcc gggtgcataa actcgttgcg gtggacgaag gacccgtcgc gttgcatcgc
     1141 gatgagcatt ttaacacggt attgctgcat aggggccata aagaagatcg gcccttgcgc
     1201 tacttaaact tgagagatcg gggcatggca gggtagccac agccccaaaa aaggcagaat
     1261 ttaacgatcg agtatcgtgc tgaggtcgat atccgcgtcg tctttttcgc caccttcgcg
     1321 gccatcagcg ccgagactgg taatcttggc ctggctgcct tctacattgt atttgaaccg
     1381 gtttttccac gggtcaagca ggtagcgctt gtccatgtac gggccagacc agccttcgag
     1441 tcccgggggt tgtgtcacga gggcatcgag accttcttca gatgtcggaa cgcggccaaa
     1501 cttgtttttg aactcgaaca gggcgaactg tatcttgccg taggtgacct tgagctcgac
     1561 ctcggccttg gcgcgcgatg ccttgtcgtc tttcagcaca acgagcagga cgcccattaa
     1621 tccacccagg atcaccagaa cgaccataag ttcaatcagg gtgaaacctt ttcgtttgcg
     1681 tagcgcttta attctcatgc gcttgcctga cgatcgatgg cctcacgaca aggttttttt
     1741 taatccaggc gcttgcatgc tgtcaagcag ccgtgaatga ggcaatcctg cttaaaatgc
     1801 tttgctcgcc ggatacgaaa cggatattta cctatgcgaa aggccttcgg cagtcttatg
     1861 gcgcttatca tcagcgccct cctgatttcg agcattatct cattttttac ccgcgattca
     1921 ttcgatacac tgccagtgaa ggcgtccaat gcaccggtta agctgacgcg ccgtatgcag
     1981 gaaacctcgt tcatggtgtc tgctgaatat gtctcgcgtt acgacttcaa ctggctggca
     2041 gaaaagctgc agctgccgaa agaaatcacg caccgcattc ttcctgttcc tgccaatttc
     2101 tacatctcaa accaactcgc gcacgctgaa tacccgtttg ccggcctcga agttgtcgac
     2161 gccgaatgta acgtaaaagc cgaacggatt ccggcgctgc gccggtattt caagtccctg
     2221 caggtttaca cgagcgaagc caagaagacc ccgctgcaca ttgcctcttt ccgttcaatg
     2281 ccagagggca taaccctgcg ctatacagac aactcggcac gcgcggcaaa atgccttgcc
     2341 gcacaggttg tggcgctggg tgaaatctcg tttctgcagg attcagtttc gctggtattg
     2401 cctgcttacc agtcagtacg ggtcaacctg gctccctatg aacgcactgc actgctcgcg
     2461 caggctggac gcaacatcta caatgcgctg gcagaagcgc acaagaaaaa tccggcacac
     2521 cgggtgcgcg tcctgcttac caacatggtc ccttacggtc atgcacaggt tttaccgttt
     2581 agcgaactcg ctggccagtt cgtgggatta acccctgaag aactcacggc gatccgtaac
     2641 gctgcggtcg ccgcgaatgg ttcggttgtt tttgtgcccg atctcgccaa gcgcatcgaa
     2701 ctgggtctcg atacctcggt catttggcag ctctggcaga cagcaaaact gccgttctat
     2761 aaagacgctt cgggcgagcc attgctctac gaagacatgg tgctcgcgct gcgccgctcg
     2821 ttgaccgaaa cagatggcgc aggcgtggta caaaaccgtc ggctgaaggc aatctatgct
     2881 tcttatgcgc gcgacaacga cgagaagcgc aagtggggcg agagtatcaa cctgccatac
     2941 cagcgcgcga gtactgaacg cctgcagaag ctgcaggtgc agatgctgct cgacgtgtac
     3001 gatttttctg cggtcggact cgaagccgaa acctacattg cgcttgaaat gcctgcgcga
     3061 gagcgttttc ccctgctata catgactgta cgtccattgg cgaagaagct gggattcgac
     3121 gagaaaaaag acggctcgga cagtgccgag atcgaaggtt cgctgcaact gctgcgcagc
     3181 gcgctggccc aggccggcaa atagccagtg ctgcaccctt acacggcgct cgtttggggc
     3241 atgggagctt cgctcctctg tttttacgca gctttttttt accggaagag aaataacctc
     3301 aggcaccgcc agctggccgg gctcggcatt ctgctgaatc ttatcagcag cgtctatttg
     3361 atctatgctg tgcgggcagt cggcgttgag atgccctcag agtattcgtt gcccgtcatc
     3421 accgcccatc gtatctttgc tacgctgatg gcgcttgcca tgcttgccat ggcagccagc
     3481 ggtattcgcc gcagggttga attccaccgc aggctcagct ggttcttttt accgggttac
     3541 acactgactt atttttccgg aatggtgatc ttccacggct aaatttttcg ttaggcggag
     3601 caaacaatgg actttatcgt aggcaacgag gcagagaaaa aacaactcga ggcaatctgc
     3661 agcaaacaag cggcagacgt aaccgagagc gatctcaaat ttatcgcccg ccgtatccgg
     3721 cagaacgcgg tcgagatggt atttcgcgcc aactcgggcc atccaggcgg cccgctgggt
     3781 ctggcagata tctatgcata cctctatttt aagatcctca acctcaaggg cgagcaccgc
     3841 gaccgcctgc tgatcagcaa cggccacgtt tgcgccgtcc gctacagcgc gatggcagtg
     3901 cgggggcagt tgcctgaatc tgagctcgct acgtttcgcc gcctcggttc acgcctgcag
     3961 gggcatccat cgaccaagta tctgcccgag gtagaaaact cgagcggttc actcggtcag
     4021 ggccttgcca acgcgagcgg cctggctctg ggcctgcgcc tgcagaagag cccggcgcgc
     4081 gtattcctgg gaatgtctga cggcgaatgc caggaaggta tgacatggga agccgcgatg
     4141 gctgctgccc acaataaact gacaaatatt catccgttcc tcgacttcaa taatatccag
     4201 atcgacggca aggtcgagaa agtgatgggt gttgagccac tagacaagaa gtttgagacc
     4261 ttcggctgga aagtccgcgt cgccagcgga catgatttcg gtgaaattgc cgccgcgttt
     4321 gactggtcgc tgaaagccga gggagctccc aagatgatcc tgttcaggac cgtattgggc
     4381 aagggtgttt cttttatgga aaacaaccct gactggcacg gtacaccgcc gaaccagaaa
     4441 gaccgcgatg cagcgatgat tgaattgtct gcatagcaga caattcaatc atctgattac
     4501 cgaacaatta ttctaaaaat aaacggtaca atcaataccc cgttgccgtc gagtagttct
     4561 tttggcggcg ggccgaagtc gtgtgcagcg tagagcgcct caatacatga gcggtcgagt
     4621 gactggtagc cgagtgatga caccagcttt gcatctttta ctacgccatt ctcatcgagc
     4681 agaaagtgga tgtagatatc ctggctgggg aatgtactga aacgcgtata tccggcagcg
     4741 tagttcatgc ggtggtacga atcgccgaaa gtcgggtagg ggtcaccgcc tggaggagcc
     4801 cacacgctct gtatcttgct gatcatcgag cgaaaatagc tatagtgggc aaaacgtgcg
     4861 gttggtatcg ccggccggcc caggtaatcc catgacaggg caaaatcatg gcgaaaccgg
     4921 taataggcag gtatacgcag gcgctccgcc gaggctgact ggctcgtttg ttcctgcggg
     4981 gcggcaatgg tcacgcggct ttctttggcg gtgagaatct tcgcgagctt tttccccgaa
     5041 acgatgcgtc cctgcatcgg ctgcatttca ccgggcgaaa gtacatagtc ggtcgagatc
     5101 gattcaaagc ccttttcttt cgtcagctgc ccctgtgcct ctgattcttt gtccgagagg
     5161 aatttgaccg ttttgcgttt tttggcctgc acaggctgag cattctctag cagcgcgcgg
     5221 ataatttctg tgttttggcc tttttcgctc tctgagtcgc caaagatcag tttgcgcagg
     5281 tctagctcaa gaaatagtgc cgtgaggata aacagcgaga gaaatgcaga aaaaaaccgc
     5341 gggaaagcgt gctcagaaat ccgcttcagc gggctcaaac ttaccgcgct ttctgccgat
     5401 ggcttcacgt agttcactcg cctctttcgg tacggtttca cccgaccgcc tgatatacag
     5461 gtcggtaagg gcgagtgctc ccgtatagtc atttaacgcc gcactcaccc gggcgcagtt
     5521 gaagaggtat ttgttgttct ggcttttttg ccaggcgacc cgctggcaaa gcttgcctgc
     5581 ggctttcaga tctttcatcg tgacgacgta gagtttcagc agaaagtact ctgtggtttc
     5641 atcgctgccg tgtccttcaa gatatgcggt cagcacttcg cgggcgcggt ctttctcttt
     5701 caatttcagg tagatttcgg cctggcgcct cacgttctgt ccatcggcca gcctctgggc
     5761 ggctgtgatg cgggcaagat tctgcagggc ttcttcttta tctttgcggt cggcaaaaat
     5821 ctcggcgact cgcaggaacc cgcgcacccg gtatttttcc ggcagcgaag acttttgcgt
     5881 cgcctggcgc agcattgcca atgctatctc ctgcgtgatc tgttcgtcgg cgagcagcat
     5941 acccttgccg taaagggcaa gcggctcctg tgcg
//
LOCUS       DAOQJF010000201         5974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7543_length_5974_cov_3.710255, whole genome shotgun sequence.
ACCESSION   DAOQJF010000201 DAOQJF010000000
VERSION     DAOQJF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7543_length_5974_cov_3.710255"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLF85_10950"
     CDS             <1..538
                     /locus_tag="PLF85_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HNE20229.1"
                     /translation="YFNRGAEHVLFRDELDKLTQRGNIRVVHILDTDVDGRYSRAQLE
                     RHVPDFKDRAIYACAPRVMLDLIEKDLREQSYNFSNYHTEQFVAQQAAKLPKSGKTHK
                     VRLLTSRKETELDENETLLSAIESAGIVIPTGCRAGMCKACTVRLDSGATDKHQKGFQ
                     GLITTCNNYPRSSVDLWV"
     gene            complement(552..773)
                     /locus_tag="PLF85_10955"
     CDS             complement(552..773)
                     /locus_tag="PLF85_10955"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HNE20230.1"
                     /translation="MKVHYDHQTDSATILFNEHAVADGNECISENVFAMYDKDNAIIG
                     IEIMGNAQKLLSMKSLEFVGVNPEQILTH"
     gene            complement(770..1012)
                     /locus_tag="PLF85_10960"
     CDS             complement(770..1012)
                     /locus_tag="PLF85_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20231.1"
                     /translation="MKFSSYFLNIARRNHPEATEETIKLALQNRIEERRQENGWWQVI
                     CHLPDRNRIMRVILLDDKETVQNAFYDRTEARRRKQ"
     gene            complement(1014..2087)
                     /locus_tag="PLF85_10965"
     CDS             complement(1014..2087)
                     /locus_tag="PLF85_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20232.1"
                     /translation="MSAKDNKAAKAIVRALGRWYETEKDRYEFRHNRNPYRTWITEIF
                     LQQTQINAGREKLKVFLKRFPDVKTLAAGPIDDVLTAFRGMGYYSRARNMFRAAQYVM
                     ANHAATMPVTYSDLKKIPGIGHYTAAIIASIHNNETVLANDANHARVLSRLYSIEHAQ
                     GSPAFAARVHELASPLFKAGMTPGDLNEALMQWGQNICKKNPRCEVCFAREYCAAYAE
                     KTQQTYPVKKARAQAFDVEWTMLVCRRGSKYQVFEAGRSFPFLKGELMFPGFLELPPH
                     KKELATPERLPSALRRKVGKIAAALPVDFRHAITRYKIAVKLVIMNDGIGGGEFFTPA
                     ELRARCHSSLMQKALSRLDKLEF"
     gene            complement(2071..3384)
                     /locus_tag="PLF85_10970"
     CDS             complement(2071..3384)
                     /locus_tag="PLF85_10970"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNE20233.1"
                     /translation="MTSSVASGGGIIATFINPNAQGGLNYYPRACLLWNAAGVITYFG
                     AEIPGPEKLAHHEILLREQDIVLPGLIDLHTHLPQYEFAAQGAAELLPWLERYTYPQE
                     SRFADARVAEQQSSNFFMSCLQHGTTTVVAYLSSFAPAAEIAFAAASAAGIRAYLGLT
                     LMDRNTPEALTTTTARAEREMLALVEKYHRKGRNEFVVTPRFAISCSEAMLQLCGEMS
                     RAHNTLLQTHISENKSEIEETLRLFPGAASYTDVYDRFGCLHERTLLGHGIHLSEAEL
                     NLISAQKSVIVHCPVSNNFLGSGIMPYQLYIQRGIRTGLGTDVAAGYSLSMLNEARQM
                     TEMAKLLSHFRAQDMRVGAERAVYQATLGNALCLGRNDLGCFEPGRRADLVVIDDSRC
                     DTMQPPLANQYTLPAERLMRVLYRSTPNMVKETLIDGKEVFVRER"
     gene            complement(3381..4139)
                     /locus_tag="PLF85_10975"
     CDS             complement(3381..4139)
                     /locus_tag="PLF85_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE20234.1"
                     /translation="MAAAVLAENLSLSINGKDILRQITLSVEAGETLVLMGKNGSGKT
                     MLLKTLVGLFTPQQGKALILGQDVHRLAHGDLDALRRKIGYVFQKSGLFDSLHVWENV
                     VFALRRFSDLNEHELRAKATDCLNRSGLKDVEDKYPSELSGGMQKRAGIARAIAMDPG
                     ILLFDDPTAGLDPVLTDAIAELIKAIKRNLGSTAIVVAHDFNLAYKLADKIGLMVSGN
                     LHTVMTRDEFRTTSEPYVEQFREGKLTGPIPVIE"
     gene            complement(4139..4891)
                     /locus_tag="PLF85_10980"
     CDS             complement(4139..4891)
                     /locus_tag="PLF85_10980"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNE20235.1"
                     /translation="MAATYPIRKNEFRAFAVSLAVHALLFLLIYLVAGKNISSLLEVS
                     IVVERGQIQPEQLDEPEETLRPQPKKSRRKLRTAREKSQAKSGRQAQRDNRTLQDPWS
                     SYEQRMFAQNRTSSGTNKGSTTTESTRWGSEKTGRTEKRGESEQVNVPPGRSDSSTRW
                     RRGAARRLLSLPAIDYPESVRRKSGQGSVELLIEIDERGHVESVEILKSSGITRLDLN
                     ARNAYRRAVFSPSASGESATGVVTVTFRMKDN"
     gene            complement(4891..>5974)
                     /locus_tag="PLF85_10985"
     CDS             complement(4891..>5974)
                     /locus_tag="PLF85_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE20236.1"
                     /translation="LSQRATDSVEAVRERVTAFTLAREGATSVFVRGTTEGINLLAHC
                     LTSPASQLSSFYSSYASPKKPAILISESEHHANIVPWQMAAAKQGYALLYVSVDGEGR
                     YKYEEDFLRSLTETYALRVIALSLQSNVTGIVHDLTPFRELARAQGAAFVIDAAQAIV
                     HVPQLIHSLAPDFAVFSGHKLFSSTGIGAVVGDKAVLDACDVYQGGGAMISLVEKERS
                     TYLDAPSRFEAGTLAVGEIYALGAAIEFAEQHRAILHTADQKLTQYADEKFRSAGIRV
                     FGIEAPHRSAIYSFQIDGVHAHDTGTLLDEQDICIRAGHHCCQVLMRALGVPATARAS
                     FAAYNTRDDVDRLIEGIGYVRKIFRK"
BASE COUNT         1184 a         1659 c         1752 g         1379 t
ORIGIN      
        1 ttacttcaac cgcggcgcag aacacgtgct ctttcgcgat gagttggaca aactcaccca
       61 gcgcggcaac atccgcgttg tgcacattct cgacaccgac gtggatggcc gttattcgcg
      121 tgcgcaactt gagcgccatg tgcccgattt caaggaccgc gccatctatg cctgcgcccc
      181 gcgcgtgatg ctcgacctga tagaaaaaga cctccgggaa cagagctaca atttcagtaa
      241 ctaccacacc gagcagttcg ttgcgcagca ggcggcaaag ctaccgaaaa gcggtaaaac
      301 gcacaaggtg cggcttctga cctcgcgcaa agagaccgag cttgacgaaa atgaaacgct
      361 gctgtcggcg attgaaagtg ccggcatcgt gataccgacc ggttgccgcg ccggcatgtg
      421 caaggcctgc acggtgcgcc tcgactcagg tgccaccgac aagcaccaga agggtttcca
      481 ggggttgatt accacgtgca acaactaccc gcgctcgagt gtcgacctgt gggtttgaat
      541 tcagaccctt gctagtgagt gagtatctgc tcggggttta cgcccacgaa ctccagtgat
      601 ttcatgctca acagcttctg tgcattgccc atgatctcga taccgataat tgcattgtcc
      661 ttgtcgtaca tcgcgaatac attttctgaa atgcactcat taccatcggc gacggcatgt
      721 tcgttgaaca ggattgttgc agaatctgtt tggtggtcgt agtggacttt cactgctttc
      781 tccgccttgc ttcggtgcga tcgtagaatg cattttgcac ggtttccttg tcgtcaagca
      841 ggattacccg catgatgcga ttgcggtcgg gtagatgaca aattacctgc caccagccat
      901 tttcctgtct ccgttcttca atgcgatttt gcaatgctag tttgattgtt tcttctgtgg
      961 cctcagggtg atttcggcga gctatgttca gaaagtagct cgaaaatttc atgctagaat
     1021 tccagcttgt ccagtcgcga gagcgccttc tgcatcaggc ttgagtggca gcgcgccctg
     1081 agttcagccg gcgtgaagaa ttcgccgccg ccgatgccgt cgttcatgat gaccaacttc
     1141 acggcaatct tgtagcgcgt gatcgcgtgg cgaaagtcca caggcagcgc cgcggcaatt
     1201 ttgccgacct tgcggcgcag cgctgaaggc agccgctcgg gtgtggcgag ttctttctta
     1261 tgcggcggta gttcgagaaa acccgggaac atgagttcac ccttgagaaa gggaaagctc
     1321 cgcccggcct cgaaaacctg gtacttgctg ccgcgccggc agacgagcat cgtccactcg
     1381 acgtcaaacg cctgcgcacg ggctttcttg accgggtagg tctgctgggt tttttcagca
     1441 taggcggcgc agtattcgcg cgcaaagcag acttcgcagc gcgggttctt cttgcagata
     1501 ttttgtcccc actgcatgag cgcttcgttg aggtcgcccg gcgtcatgcc ggccttgaaa
     1561 agcggggatg ccagttcgtg aacgcgcgcg gcaaacgccg ggctgccctg cgcgtgctca
     1621 attgaataaa gccgcgacag cacacgcgca tggttagcgt cgttggcgag tactgtttcg
     1681 ttgttatgga ttgaagcgat gatcgccgcg gtgtaatggc caataccagg tatcttcttc
     1741 aggtcggagt acgtaacagg cattgtcgcc gcatgattgg ccatcacgta ctgcgcagcg
     1801 cgaaacatat tgcgcgcgcg cgaatagtag cccatgccac gaaacgccgt aagcacatcg
     1861 tctattggcc ccgcggcaag tgtcttcaca tcgggaaagc gcttcaggaa taccttgagt
     1921 ttttcgcgac ccgcattgat ctgcgtctgc tgcagaaata tctcggtaat ccaggtgcgg
     1981 tatggattgc ggttatgtcg gaattcgtag cggtcttttt cggtttcgta ccagcgcccg
     2041 agcgcgcgca cgattgcctt tgccgcctta ttatctttcg cggacaaaaa cttccttccc
     2101 atcaatcaga gtctctttca ccatgtttgg tgtgctgcgg tagagcacgc gcatcagccg
     2161 ctcggccggc agcgtgtatt ggtttgccag tggcggctgc atggtatcgc agcggctgtc
     2221 atcgatcacg acaagatcgg ctcggcggcc gggttcgaaa cagcccaggt cgttgcggcc
     2281 gaggcagagt gcattgccga gggttgcctg gtagacagcg cgttcggcgc cgacccgcat
     2341 gtcttgtgcg cgaaagtggc tcagcagttt tgccatctct gtcatctgcc gggcctcatt
     2401 gagcatcgag agcgaataac ctgccgcgac gtccgtgccg agtcctgtgc gaatacctcg
     2461 ctgaatatac agttggtatg gcatgattcc gctgcccagg aaattgttag agactggaca
     2521 atgcacgatc acgctctttt gagcgctgat cagattcaac tccgcctcgc tgaggtgaat
     2581 accatggccg agcagcgtcc tttcgtgcag gcagccaaag cgatcataga cgtcggtata
     2641 gcttgcggca ccaggaaaca ggcggagtgt ttcttcaatc tctgacttgt tttcgctgat
     2701 gtgggtttgc agcagcgtgt tatgtgcgcg gctcatttcg ccgcagagtt gcagcatggc
     2761 ttcgctgcag ctgatcgcga agcgcggggt aacgacaaat tcattgcgac ctttgcggtg
     2821 gtatttttcg accagcgcca gcatctcgcg ttctgcgcgc gccgtcgtgg tggtgagcgc
     2881 ttcaggtgta ttacggtcca tgagcgtcag gcccaggtag gccctgattc ccgcagcgct
     2941 ggctgcagcg aaggcaattt cagccgcggg cgcaaatgat gacaggtagg caacgacggt
     3001 cgtggtgccg tgttgcaggc agctcatgaa gaagttggag ctctgttgtt ctgcgacccg
     3061 cgcgtcggcg aaccggcttt cctgcggata cgtgtagcgc tcaagccagg gcagtagttc
     3121 cgctgcgccc tgtgcagcga actcgtactg cggcaggtgc gtgtgcaggt cgatcaaacc
     3181 tggcaggacg atatcctgtt cgcgcagcaa tatttcgtgg tgcgccagtt tctcgggccc
     3241 gggtatttcg gctccgaagt aggtgatgac tcctgctgcg ttccagagca ggcaggcgcg
     3301 cgggtagtag ttgagtccgc cctgtgcgtt ggggttgatg aaggtggcaa taatgccgcc
     3361 gccgctggca acgctgcttg tcattctatt accggaatcg gccccgtcag ttttccttcg
     3421 cggaactgct cgacgtaagg ttctgaagtg gtacgaaatt catcgcgggt catcacggtg
     3481 tgcaggttgc cggaaaccat gaggccgatt ttgtcggcga gtttgtacgc aaggttaaag
     3541 tcgtgtgcga cgacgatcgc ggtactgccg aggttgcgct tgatagcctt gatcagttcg
     3601 gcaatggcgt cggtcagaac cgggtcaagc cccgccgtcg ggtcgtcaaa cagcagaatc
     3661 ccgggatcca tcgcgattgc acgcgcaatg ccggcgcgct tctgcattcc cccggaaagt
     3721 tctgacgggt atttgtcttc tacatctttc aggcctgaac ggttcaggca gtctgtggct
     3781 ttcgcccgca gttcgtgttc gttcaggtcg ctgaagcggc gcagcgcaaa caccacgttt
     3841 tcccagacgt gcagagaatc aaagagcccc gatttctgga aaacataacc gatcttgcgg
     3901 cgcagcgcgt cgagatcccc atgggccagg cggtgcacat cctggccgag aatcagggct
     3961 ttaccctgct gcggggtaaa aagaccgacc agggttttga gcagcatcgt cttacccgag
     4021 ccgtttttgc ccatgagtac gagggtttcg cccgcttcga cgcttaaagt aatctgcctg
     4081 agtatatctt tgccgttaat cgacagcgaa agattttctg ccagtaccgc cgcagccatc
     4141 agttgtcctt catgcggaaa gttacggtga cgacaccggt ggcgctttca cccgaagccg
     4201 agggtgaaaa cacggcccgg cggtaagcgt tacgggcatt gaggtcgagt cgcgtgatgc
     4261 ctgaagactt caagatctcc acactttcaa cgtggccgcg ctcgtcgatc tcgatgagca
     4321 attcaacgct accctggccc gatttgcgtc gtacgctttc cgggtagtcg atcgccggca
     4381 ggctaagaag ccttcttgcg gcacctctgc gccagcgcgt tgaagaatcg ctgcggcccg
     4441 gagggacatt gacctgttcg ctctcgcccc gtttttccgt gcggccggtt ttttcgctgc
     4501 cccagcgcgt agattctgtc gttgtgctgc ccttgtttgt gccggagctc gtgcggttct
     4561 gcgcaaacat ccgctgttcg tagctgctcc agggatcctg cagggtacgg ttgtcgcgct
     4621 gcgcctgccg cccgctcttt gcctgcgact tttcgcgggc tgtccgtaat ttccgcctgc
     4681 ttttcttcgg ctgcggtcga agagtttctt cgggttcgtc gagttgttcg ggctgaattt
     4741 gcccgcgttc cactacgata gagacttcga gcaaagagga aatatttttt ccggcaacga
     4801 gataaatcag cagaaacagc agcgcgtgca ccgccagcga gacggcaaaa gcgcggaatt
     4861 catttttcct gatggggtag gtcgcggcca ttactttcgg aatatcttcc gaacgtatcc
     4921 gattccttcg atgagcctgt caacatcgtc tcgggtgttg taggcagcga aggaagcgcg
     4981 tgcagttgcg ggaacgccca gcgcacgcat gagaacctgg cagcaatggt gccctgcacg
     5041 gatgcagata tcctgctcgt cgagcagtgt gcccgtatcg tgcgcgtgca ccccgtcgat
     5101 ctggaaagag tagattgctg aacggtgcgg cgcctcgatg ccaaagaccc gtatgccggc
     5161 tgaccggaac ttttcatcgg cgtactgcgt cagtttctgg tcggcagtat gcagtattgc
     5221 ccggtgttgc tcggcgaatt cgatggcagc gccgagcgcg taaatttcgc cgacggcaag
     5281 cgtgccagct tcgaaacgag acggggcatc gaggtaggtc gagcgttcct tctccaccag
     5341 gctgatcatg gcgccgccac cctggtagac gtcgcatgcg tcgagcactg ccttatcgcc
     5401 aacgactgcg ccaatacccg tgctcgaaaa aagtttgtga cctgagaata cggcaaagtc
     5461 cggggccagt gagtggataa gctgcggtac atggacaatg gcctgtgcag catcgataac
     5521 aaaagcagct ccctgcgcac gcgccagttc gcgaaaagga gtaagatcat gtacaattcc
     5581 tgttacattg gactgcaggc tgagcgcgat gacgcgcagc gcatacgttt cagtcaggga
     5641 acgaagaaaa tcctcctcgt acttgtagcg cccctcaccg tcgaccgaga catagagcaa
     5701 ggcataaccc tgcttcgctg cggccatctg ccaaggcaca atattcgcat ggtgttcact
     5761 ctcgctgatg agaattgcgg gcttcttggg cgaggcatag gagctgtaaa aagacgatag
     5821 ctgcgaagcc ggtgaagtca gacaatgcgc cagcaggttg atgccctcgg tagtgccgcg
     5881 cacgaaaact gaagtcgcgc cctcgcgcgc gagcgtgaaa gcagtcacgc gttcgcgtac
     5941 cgcttcgaca ctgtcggtag cgcgctgcga gagc
//
LOCUS       DAOQJF010000202         5945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7595_length_5945_cov_5.205603, whole genome shotgun sequence.
ACCESSION   DAOQJF010000202 DAOQJF010000000
VERSION     DAOQJF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7595_length_5945_cov_5.205603"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PLF85_10990"
     CDS             <1..1396
                     /locus_tag="PLF85_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20237.1"
                     /translation="MRMSFLSVFFPLLLIAVSSLAANTEVCGPDAQKAAGLAQNGDAA
                     TAIWCYGQSKKWGEIIALQGAVVPSGLARYGEGNYALVISNLALAHLETGNLQAGRRV
                     LEQGMRQLMKSPQVVHTAYNVSKLFQDPTMRFAERQRWHFLLLEAMQQPRPNNQLADC
                     LTANTYFGVDQGVRERKIREAHLMARVAIERHDADMVRHWGYLRDMIQIQALALGHDL
                     RPSQPKRKLKMLFVVVPETRLATGHPVHGNVDAKLDEADLQELLFNFRYFASAFQALT
                     GIGWDFKIIRHTGAVTKTTYVSDPPRSVMHPIMESITPAFSALTLREIEQSDAVTLVW
                     AGTRQPAGHLITNGSGTEYGFPIKGKSLHRLLVISDSNKRYLDGNHANSALFWFHEIF
                     HVMEWAYYKSPFPSAEHSYARRDAWPADYQGTTEWDFYRETYLKRFMPEDSLARFEWR
                     SNHEGFYNRKVKGK"
     gene            1393..2541
                     /locus_tag="PLF85_10995"
     CDS             1393..2541
                     /locus_tag="PLF85_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE20238.1"
                     /translation="MTFVKEAELRAKEANEAKNRFLSNISHELRTPLNGVMGFAQLIS
                     MSPGSDISVREYAEHIHASAEHLLGLINELLDFSRIEAGTLSIEVRQSTLDEVLRTVV
                     ELVHVRARQKGIQFESALAVSHSQVLYTDVRRLRQVLLKLLSNAVKFTRHGRVALRVF
                     ERENRLHFEVHDEGIGIAQENLSMLFQPFSQVGDTSFRSQGLGLGLAISQQIVQALGG
                     EITVRSALGQGSVFSFSLPCLQASGLQQPVGATLQLPPYFKKLKVLYVEDDEHGRELM
                     QEFLRRSQIELYVAEDAGTAMEIGKQANPDLVLLDLNLPDMSGFDLMARIRQTPWGSD
                     KPVIAVSADHLPPTRERAFDSGVAEFVAKPIDLQLLQQVIGRFAEQLP"
     gene            complement(2568..5429)
                     /gene="gcvP"
                     /locus_tag="PLF85_11000"
                     /pseudo
     CDS             complement(2568..5429)
                     /gene="gcvP"
                     /locus_tag="PLF85_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
BASE COUNT         1261 a         1644 c         1675 g         1365 t
ORIGIN      
        1 catgcgcatg tcttttctgt ccgtattttt tcctctgctg cttattgcag tgtcatcact
       61 ggctgccaat acggaagtct gcggacccga cgcgcaaaag gcagcggggc tggcgcaaaa
      121 cggtgatgcc gcgaccgcta tctggtgtta tgggcagagc aagaaatggg gtgaaatcat
      181 cgcgctgcag ggcgccgtgg taccctcggg tctggcgcgc tacggtgaag gtaactatgc
      241 actggtaatc agtaacctgg cgctggcaca cctcgaaacc ggtaacctgc aggcgggccg
      301 gcgcgtgctc gaacagggta tgcgccagct gatgaaaagc ccgcaggttg tgcacaccgc
      361 gtacaatgtt tccaaactct ttcaagaccc gacgatgcgt tttgccgagc gccagcgatg
      421 gcattttctg ctgttagaag caatgcagca accgcgaccg aataaccagc tggccgattg
      481 tcttactgcc aatacctatt ttggcgtcga ccagggcgtg cgtgaacgaa aaatccgcga
      541 agcgcatctt atggcacggg tcgctatcga gcgacacgat gccgatatgg tgcgccactg
      601 gggttacctg cgcgacatga tacagattca ggcgctggca ctgggacatg atctcagacc
      661 ctcgcagccg aagcgcaaat tgaaaatgct cttcgtcgtc gtgcctgaaa cgcggctggc
      721 aactggtcac cccgtgcacg gcaatgtcga cgcaaaactc gatgaagccg atctgcagga
      781 actacttttt aatttcaggt actttgcctc tgccttccag gcgctcaccg gcattggttg
      841 ggatttcaaa atcatacgcc acacgggcgc cgtaaccaaa acaacctacg tctcagaccc
      901 gccgcgctcg gtgatgcacc cgatcatgga atcgattact cctgcattca gcgcactcac
      961 actgcgcgag attgaacaga gcgacgcagt gacgctcgtt tgggccggta cgcgccagcc
     1021 ggcaggtcac ctgatcacga acggcagcgg caccgagtat ggtttcccga tcaagggcaa
     1081 gagcctgcac cgcctcctcg tgataagcga ttctaacaag cgctaccttg acggcaacca
     1141 cgcgaactca gcgctcttct ggtttcacga aatttttcac gtcatggaat gggcgtacta
     1201 caaatcaccg ttcccgtcgg cagaacattc atatgcgcgg cgcgacgcct ggcctgcaga
     1261 ctaccaaggc acaaccgaat gggatttcta ccgcgagacg tacctgaagc gttttatgcc
     1321 agaagacagc ctcgcgcgct tcgagtggcg cagcaaccac gaaggatttt ataaccgcaa
     1381 ggtgaaaggc aagtgacatt tgttaaagaa gcagagttgc gcgccaaaga ggcaaacgag
     1441 gccaagaatc gctttctttc aaatatcagc cacgagctta gaactccgct caacggcgtc
     1501 atgggctttg cgcagctcat ctcgatgagt cccggcagcg atatatctgt ccgcgagtac
     1561 gccgagcata ttcatgccag cgccgaacat ttgctgggat taatcaatga actgctcgat
     1621 ttcagccgca tcgaagccgg tacgctcagc attgaggtgc ggcagtctac actggatgaa
     1681 gttctgcgta ctgtcgtcga actggttcac gtacgggcgc ggcagaaggg catccagttt
     1741 gaaagcgcgc tggcagtttc gcactcgcag gtactctata cagatgttcg ccgtctgcgc
     1801 caggtgctac ttaaactgtt gagtaatgct gtgaagttta cacgccatgg ccgtgtcgcg
     1861 ttgcgcgttt ttgagagaga aaaccgcctg cactttgaag tacacgatga aggtataggt
     1921 attgcccagg aaaatctctc catgctgttc cagccttttt cgcaggtcgg ggatacaagt
     1981 ttccgcagtc agggacttgg tcttggcctg gcgatcagcc agcaaatcgt tcaggccctg
     2041 ggcggtgaga taactgtaag gagcgccctg ggacagggga gtgttttttc tttcagccta
     2101 ccttgcctgc aggcgtcagg tctacagcag ccggttggcg cgacgctgca attgccgccg
     2161 tatttcaaga agctgaaggt attgtacgtt gaggacgatg agcatggtcg cgagctcatg
     2221 caggagttcc tgcgccgttc acagatagaa ctctatgtgg ctgaagacgc cggcaccgcg
     2281 atggaaatcg gaaaacaggc taatcccgat ctcgttctgc tcgatttgaa tttgcctgac
     2341 atgtcagggt tcgaccttat ggcgcgcata cgacagactc catggggatc tgacaagccc
     2401 gtcattgcag tctcagccga tcacctgccg cctacccgtg agcgggcttt cgactcaggt
     2461 gtagcagagt tcgtagccaa gccaatcgac ctgcagttgc tgcagcaggt gattggcagg
     2521 tttgctgaac agttgccata gtgacagtgg caactttgac ttaactgcta cttctcgtac
     2581 gcttcaagcg gcagacacgc acagatgagg ttgcggtcgc cgtgcgtgtt atcaatacgg
     2641 ccgactgaag gccagaactt gttggcgcgc acatagtcga gcgggtacgc ggcctgctcg
     2701 cgcgagtaac tacgtttcca ttcgcttgtc acaaccatcg tcgctgcatg cggcgcgcct
     2761 ttgagcgggt tgtctgtccg gtctgattta ccttcttcga tggcggcgat ctctttgcga
     2821 attgcgatca tcgcatcgca gaaccggtcg agctccgcct tgggttcact ttcggtgggt
     2881 tcgaccatga ttgtgccggc caccggaaag ctcatcgtcg gtgcatgaaa gttatagtcc
     2941 atcagccgtt tggcaatgtc ttccgcttcg atgccggtcg tggctttgaa gacgcgggtg
     3001 tcgagaatca tctcgtgtgc gcagcggccc tggcttccgg taaagagcac cgggtagtgt
     3061 ttctctagcc gcgccttgat atagttcgcg ttgagaatgg caaacttcgt cgctttctcg
     3121 agaccttcag cgcccatcat gcgaatgtac gcgtacgaaa tcagcagaat cgaagcgctg
     3181 ccccatggtg cggcgctgat ggcggaaatt cgtcggctgg tgagcgcagt cgaaccaccg
     3241 acgcttttgc caccggcgtc catctctacg accacatgcc ccggtaagaa agggacgagg
     3301 tgtctggcga cgccgatcgg ccccatgccc gggccaccac cgccgtgggg tatagcaaac
     3361 gtcttgtgca ggttcaggtg gcagacatcg gcgccgatat tcgccgggct cgtgaggcca
     3421 acctgcgcgt tcatgttggc gccatccatg tagacctggc cgccgttggc gtgaatgatc
     3481 tcgcacacct cggcgatgcg ctcttcgaaa atgccgtacg ttgacgggta ggtgaccatc
     3541 aggcaggcca ggttggcttt atgttcttcg gccttcgccc gcaggtcgtc gatgtcgata
     3601 taacctttag cgtcggtctt ggtgacgacg acctgcatgc ctgccatcgc tgcactcgcc
     3661 gggtttgtac cgtgcgcaga agagggtatc agcgcgacgt tgcggtgcga ttcgccgcgc
     3721 gctttgaaat attcacgtat gaccatgagc cctgcgtact caccttgcgc gcctgaattc
     3781 ggctgcaggc tgataccggc gaaaccggtg atctgtttca ggtaatctga cagttcatcg
     3841 atgatctgtg tgtaaccctg cgcctggtca cgcggcacaa aggggtgcag caggctgaag
     3901 cccggcatcg aaacaggaat catttcggcc gtggcattga gtttcatcgt gcacgaaccc
     3961 agcgaaatca tcgagtggca gagcgaaagg tctttcgcct cgagcgactt gatgtaacgc
     4021 agcatctcat gttcgctgtg gtacgagttg aataccgggt gcgtcatgaa cgcgctctgg
     4081 cggattagcc cctgtggcaa atcaagctta tctgtctggg ccatgttgcc gccggccttg
     4141 cccgcaatca gttcgacgag agcggtaata tcagctgctg tatgtgtttc gtcgaccgat
     4201 acgcgcacgt ggttcttatc gacacggcat atattcacct gcgcggcggc gcagcgtgta
     4261 tgtacggctt cggcatcagc caccgcgatt gtcagtgtat caaaatggtg cgttgtttcg
     4321 actttgagac ctgctgcggt cagtgtttgc cgcagttggt ggctcagtcc atagattcgg
     4381 gaagcaatcc cccttaaagc tttggggccg tggtagacgc cgtacatacc ggccatcacc
     4441 gatagcagta cctgcgcggt gcagatgttc gaggtggcct tctcgcgctt gatgtgttgc
     4501 tcgcgcgtct gcagcgccat gcgcagcgca cgtttgccct ggctgtcgat cgaaacaccg
     4561 atgaggcggc cgggcatctg gcgcttgaat tcgtcgcgtg tggcaaaata gcccgcgtgt
     4621 ggcccgccgt aacccatcgg tacgccaaag cgctgcgttg aaccgacaac ggcatcggca
     4681 ccccattcac ccggcggggt caaaagcgtg agcgagagca ggtcggcgac aactgccacg
     4741 agcacgttgg ccgatttcgc cttcttcatg aaattggtat aatcgatgac ttcgccgtcg
     4801 ccggcggggt attgtaccag tgcgccgaaa aaactcgcgt cgagttcaac cttttcatgg
     4861 tcgccgatca ccagctcgac gccgatgggc gccgtgcgcg tgcgcaccac gtcaatggtc
     4921 tgcggaaaac aacgctcaga gacaaaaaac ttcttcgctg tttcgttctt tgcttcggtg
     4981 aacagcatat gcatcgcttc ggcggctgcg gttgcttcat cgagcaggct cgcgttcgcg
     5041 atttccatac ccgtgaggtc gatcaccatt gtctggtagt tgagcagcgc ctcgaggcgg
     5101 ccctgcgaaa tttctgcctg gtagggagtg tatgctgtgt accagcccgg gttctcgaaa
     5161 atgttccgca gcaccacaag cggcgtattc gtaccgtagt aaccctggcc gatgaagttc
     5221 ttgaagatct tattcttcgc cgcgataccc gaaacatggc ccagcagctc gacctcggtc
     5281 attgccgcgg gcaggtttaa tttttctttc aggcggatgt tctgcggtat cgtctcgtcg
     5341 atcagctgct cgacagatgc gaccccgatt tctttcagca ttgccgcggt atcgcgctca
     5401 gaggcgttat ggcggtcatt aaagctgtcg cggtattggc tgtcgatttg catggtttaa
     5461 agcaacgcag gcggtggatt aaacagcgcg taaagcacct gtcagccttt gggaaaggct
     5521 attcaaaaca gtttccggaa accgcttcag gtgcccactt ttggttaaca ccggaaacgg
     5581 cgggatttag tataatttta ccgcaaaatg gctaagtaaa aatacctata ccaatacgca
     5641 tgcacgcata atgaagaatt tttcatctgt ttttacaatt attacggttg accgccagcg
     5701 ctactaaagc ctgactcagg gcgctggcac aaaattccgc ggcagcggga attcggatcg
     5761 gcgaggtgaa atgcagaaat tactttcttc tccctacgag tccgcagtta tcgaggccct
     5821 cgacagcaac accatagttg cgctcaccga ccgcgccggc atcatcttac acgtcaacga
     5881 aacattctgc cgtatatctg gctatgctgc atacgaattg ctgggcaaaa cacacagcgt
     5941 cgtta
//
LOCUS       DAOQJF010000203         5941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7604_length_5941_cov_5.558614, whole genome shotgun sequence.
ACCESSION   DAOQJF010000203 DAOQJF010000000
VERSION     DAOQJF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7604_length_5941_cov_5.558614"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLF85_11005"
     CDS             <1..400
                     /locus_tag="PLF85_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20239.1"
                     /translation="LETRDSRQLIPVSVSRADLVFQTSRVAALVYLLQQETYSAADVS
                     AWRRAMDDRMHTSQRAHLIPGMFDIFEDWYRDGAYGCFLSGAGSTLLAFWPENIDIGS
                     LDLTHRLREKKIAAIQREFRVDQNGLMVLA"
     gene            452..1261
                     /locus_tag="PLF85_11010"
     CDS             452..1261
                     /locus_tag="PLF85_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNE20240.1"
                     /translation="MNMRHNPELVENLQKKLRLLNQQHGVIALKTGTEVEDMDGAEIA
                     VMRQISAAAGLPLVVKIGGPEARRDMRECLALGVDVLLAPMVESVYSLVNFVDTAKSI
                     MRETGKEARLAINLETGLAVQNLDAMIATSAFSQLSQVTIGRGDLSKSMNLSVDDEEV
                     LTVTRNTLVRLNRQNKITSVGGGLSVHNISNMSELLPAARFNTRHIVFENSEAFSRDA
                     ARNLSEGLYFEQAMYEAFAVMNPGRAEFYAERNKQLEERLPVLRIRRTAAM"
     gene            1358..2419
                     /locus_tag="PLF85_11015"
     CDS             1358..2419
                     /locus_tag="PLF85_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE20241.1"
                     /translation="MTATASSTSCAIIGGGYAGIATAIELQALGFPGKVTLFDPRPAH
                     QKITQWHKLARGDEASKYEEPFTALARKFRFAFVQQQVDVRQLLSDPGKALGATFGSI
                     VVAQGSHTPARPPNVITFDDLRDDESRQRELRTLKGRDIAVVGGGPTGVQFAFEFAER
                     GNRVILYEARDRLLPSFDYSLGAIALQAASDAGIDVRCNTEFLYYENGIVTSKSGLNE
                     LTDRTDYALFVPGVRSTPSVVCDVYGRIEGLPHQNLYAAGDNSYFSGPGLNAKSAQAA
                     VRKGQHVARTIVAALAGKAPTTYSYAELGYFVSLGPRNAVGYLLSQKTAFSGRGALFM
                     KEAIERQYNLYLAGLPVYP"
     gene            complement(2430..3344)
                     /locus_tag="PLF85_11020"
     CDS             complement(2430..3344)
                     /locus_tag="PLF85_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20242.1"
                     /translation="MFFTRSRQPLDAQFNDAQFEEIFLHNRYPGSKTQSGNFADKAEL
                     WALYDGEPDYRGLNLLRSLRFLRGIHTDLRIGPRSAANAALRGYCSDTNNDGKKSEVW
                     FEAYNGNISDATRTTIAMAPYGHADKSRALSQKSFVRFIVPLAKKKPLEKIDALMLAY
                     AIATLLDKGRFAGIIDADLFLFVPPAAARAGVSNYHRGDRLLNTHLVAGFNHFEQNGA
                     VRFFTHGLNRFGMPDVLVSAGKAVTLSTEHYQDVLREMHARFLAYLTKGPRAALEHTR
                     ALRNSKSLPEQVAAMMGKKIVELAAGGQ"
     gene            complement(3353..>5941)
                     /locus_tag="PLF85_11025"
     CDS             complement(3353..>5941)
                     /locus_tag="PLF85_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNE20243.1"
                     /translation="LAVSIVASFFTVKLFQDVRTDFATLLPENDRSVVHVKEATERTG
                     GVGNIFVSIYSPDFEANKKFAQAYAALLEKYPKDLVKFFDYNTRDTEQFFKDRLFYYL
                     GTEELKELRSTLRKRLEKTRLSAFGMDLEESNPDAQLKKVLKKIFDKYKGENPFGFNK
                     EGYFTDKTGENVAMIIRPTGDAADQKFSEAVVSRLQADIAKLDPKKFHPQMEVGLAGT
                     YVALLQNFASIIQDTVETAALTIFLVLGSIYLYYRSLRMVLMLCAGVLVGILLTFAVT
                     YFRIGYLNQQTAFLASIIVGNGINFGLILMARYLEERGHGTSFRRSYNRAIFYTISAT
                     FMAAFATSISYSILSVTTFRGFSQFGFIGGIGMVLCWAALTLIIPCFLVLFERWRPQK
                     VSKIHKFIQRDNARKLAQVIHRNRKPLTWILYVLVPIAIAGTIIYASKDRFEYSLKKL
                     SIKVSEGPGSEQYFMRRVDTILGNGSNASMLIAHDREDAHRTAQALEKKIADAKAAGK
                     PLFISSVRWLDQFYPQDQQEKLAVIKDLRRLFLPKYLSMLSPQEREWGQVALKALKAE
                     PFKTEELPEMVLRLFREVDGKIGRVVFVAASQDAVLSNILDIIQIGKEIGSSVTLAPG
                     KTLEKGQVLFASESMIFIDILANVSSEGPIVTLICFLAVGGLIWSGFRKLKEFLIVYT
                     FLSLGMFAFIATLQFFEVKLNFFNFITIPITIGIGVDYAINIYYRYKADHERSITDAV
                     ASTGSAVFLCSWTTIIGYGTMLWAKNQAMASFGLMAVIGEVSCLAFAMVFMPAWLGMR
                     EDRRKHPETSHAHKTPLVLEHALPAHTALEAEEVVKPARRVSAAARKKAPPKKKAAIR
                     RKKPSA"
BASE COUNT         1359 a         1680 c         1606 g         1296 t
ORIGIN      
        1 actcgaaacc cgtgattcac gccagctgat cccggtaagc gtctcgcgcg ctgatctggt
       61 cttccagaca tcgcgggtag ccgccctggt ttacctgctg cagcaggaaa cctactcagc
      121 cgccgacgtc tctgcctggc gccgcgcgat ggacgaccgc atgcacacct cgcagcgtgc
      181 gcacctgatt ccaggcatgt ttgatatttt tgaagactgg taccgcgacg gggcctacgg
      241 ctgctttctt tccggtgcag gttctaccct tctcgccttt tggccagaga atatcgacat
      301 aggctcgctt gacctgactc accggctgcg cgaaaagaag atcgccgcga tacagcgtga
      361 attccgggtc gaccagaacg gcctgatggt tctcgcttaa aacgcacgac ccccatttat
      421 gtcttcagcg ttgggcagaa tgctccgata aatgaacatg cggcataatc ccgaacttgt
      481 cgaaaacctg cagaaaaagc tgcggttact caaccagcag cacggcgtca ttgcgctaaa
      541 gacgggtacc gaagtcgaag atatggacgg cgcagaaatc gcagtgatgc gccagatatc
      601 tgcggcagcc ggcctgccac tcgtcgtcaa aatcggcggc cccgaagcgc gccgtgatat
      661 gcgcgaatgc ctggcgcttg gtgtcgatgt gctgctcgcc ccgatggtcg aaagcgtcta
      721 ctcgcttgtc aattttgtcg acacggcaaa atccatcatg cgggaaacgg gcaaagaagc
      781 ccgccttgca atcaacctcg aaacaggtct cgccgtccag aaccttgacg cgatgatcgc
      841 gaccagcgct ttctcacagc tgagccaggt cactatcggc cgcggcgacc tcagtaagag
      901 tatgaacctt tcggtcgacg atgaagaagt ccttaccgtc acccgcaata cgctcgtgcg
      961 actcaaccgg caaaacaaga tcacttctgt cggtggcggc ctgagcgtgc acaatatcag
     1021 caatatgtcg gagctcttgc ctgctgcgcg tttcaatacc aggcacattg tcttcgaaaa
     1081 ctccgaagca ttcagccgcg acgcagcccg taacctttct gaaggactct acttcgaaca
     1141 ggcgatgtac gaagcattcg ctgtgatgaa tcctggccgc gccgagttct acgcagagcg
     1201 taacaagcag ctcgaagaaa ggcttcccgt cctgagaatc cgccgcactg cggcgatgtg
     1261 attttttgcc acggcagcct ttcgccacgc aggtgcctgt ggccgtagca gaccaaggca
     1321 cctcctgtgc cattggtccg tcgcacagag gtcatccatg accgcgacgg cctcaagcac
     1381 atcgtgtgct attatcggtg gggggtacgc tggcattgct actgccatcg aattgcaggc
     1441 actcgggttt cccggtaaag tcacactgtt tgatccccgc ccggcccacc agaaaattac
     1501 gcagtggcat aaactggcgc gcggcgacga agccagtaag tacgaagaac ctttcacagc
     1561 gctcgcacgc aagtttcgtt ttgctttcgt gcagcaacag gtcgatgtca ggcaattgct
     1621 gagcgatccc gggaaagcac tgggagcaac gtttggtagc atcgttgtcg ctcagggttc
     1681 gcacacaccc gcgcgcccgc caaatgtcat caccttcgac gacctgcgcg acgatgaaag
     1741 ccgccagcgc gaattgcgca cccttaaagg cagagatatc gcggtcgtcg gcggcggacc
     1801 aaccggcgtg caatttgcct tcgagttcgc cgaacggggt aaccgcgtga tactctacga
     1861 agcgcgcgac cgtctgctac cctcttttga ctactcgctc ggtgcgattg cgctgcaagc
     1921 cgcaagcgat gcaggcattg acgtgcgctg caacacggaa tttctctatt atgaaaatgg
     1981 tatcgtcacc agcaaaagcg gcctgaacga gctcaccgac cggacagatt acgcgctgtt
     2041 tgttccgggt gtacgctcta caccatcggt cgtgtgcgat gtctatggcc gtatcgaagg
     2101 attgccccac cagaatctct acgccgccgg cgacaacagc tacttcagtg gcccgggcct
     2161 gaatgcaaaa agcgcgcagg ccgccgtgcg caaaggacaa cacgtcgcgc gcacgatagt
     2221 tgccgccctc gcaggaaaag ctcccactac ttacagctat gctgaactgg gctattttgt
     2281 ctcgttaggg ccgcgcaacg cggtgggata tcttctctcg cagaagacag cattttcagg
     2341 ccggggagcg ctgtttatga aagaagcgat tgaacgccaa tacaacctgt acctggcggg
     2401 tctgcccgtg tatccctgag acagcgatat tactgcccgc cggcggcgag ctcgacaatc
     2461 tttttgccca tcatggcggc aacctgctct ggcagagatt tactgttgcg caacgctctc
     2521 gtatgttcca gcgcagcacg cggacccttc gtcaggtaag caagaaaccg ggcatgcatt
     2581 tcacgcagaa catcctggta gtgttcagtg cttagcgtga ctgctttgcc ggcgctgacg
     2641 agcacatcgg gcatgccaaa cctgttcagg ccgtgcgtga aaaaccgcac tgcgccgttc
     2701 tgctcaaagt ggttgaagcc tgcaaccagg tgcgtattga gcagcctgtc gccccgatgg
     2761 tagttcgaga caccggcgcg ggctgcagcg ggtggtacaa acagaaaaag atcggcgtcg
     2821 atgataccgg caaaccggcc cttgtcgagt aacgtcgcga tcgcgtatgc cagcatcagc
     2881 gcatcaattt tctccaaagg ctttttcttt gccagcggaa caatgaagcg cacaaaagat
     2941 ttctgtgaca gcgcgcgcga tttgtcagcg tgcccatagg gtgccatcgc aatcgtcgtg
     3001 cgcgtcgcat cggagatatt tccgttatag gcctcgaacc agacttcaga ttttttgccg
     3061 tcattattag tgtcgctgca gtaaccgcgc agcgcagcat tcgcggcaga gcgcggccct
     3121 atccgcaggt cggtatggat accccgcaaa aaacgcaggc tacgcagcag gttgagtccc
     3181 cggtaatcag gttctccgtc atacagtgcc cacagctcgg ccttatcggc aaaattaccg
     3241 ctctgcgttt tactgccagg atagcggtta tgtaggaata tctcttcaaa ctgagcatcg
     3301 ttaaactgcg catcgagcgg ctgtctgctt cgggtaaaaa acatcaggta cctcaggctg
     3361 aaggtttttt gcggcggatc gcagcctttt tcttaggggg agctttcttc ctggcggcgg
     3421 cagaaaccct gcgcgcaggc ttcacaacct cttctgcctc gagggccgtg tgcgccggca
     3481 acgcatgttc cagcaccagc ggcgttttgt gagcatgcga cgtttcggga tgtttgcgtc
     3541 tgtcttcgcg cataccgagc catgccggca taaacaccat cgcgaacgcg aggcagctta
     3601 cctcaccgat cacggccatc aggccaaacg acgccatcgc ctggttcttg gcccagagca
     3661 tggttccgta gccgatgatc gttgtccacg aacagagaaa tactgcggaa cccgtgctcg
     3721 caactgcatc ggtgattgag cgttcgtgat ctgccttgta ccggtagtag atattgatag
     3781 cgtaatcgac gccgataccg atggtaatgg gtatggtgat aaagttgaaa aagttgagtt
     3841 tcacttcaaa aaactgcagc gtcgcgatga aagcgaacat ccccagcgaa aggaatgtgt
     3901 acacgatcag aaattccttc agcttacgaa atcccgacca gatcaatcca cccaccgcca
     3961 gaaagcagat gagcgtcaca atggggccct cagacgaaac attagccagg atatcgatga
     4021 agatcattga ttcgctcgca aacagcacct gacccttttc gagtgtttta cccggtgcca
     4081 gcgtcacgct gctgccaatt tctttaccga tctgtatgat atcgagaatg ttcgacaaca
     4141 ctgcatcctg gctggccgcg acaaatacca cacgcccgat tttaccgtcg acctcgcgga
     4201 acaggcgcag caccatctcg ggcaattctt cggttttaaa cggctcggcc ttcagcgcct
     4261 tgagcgccac ctgaccccat tcgcgctcct gcggtgaaag catcgaaaga tatttgggca
     4321 ggaaaagccg gcgcagatct tttatgacag ccagcttctc ctgctggtcc tgtggataga
     4381 actggtcaag ccagcgcacc gagctgatga agagcggctt gcctgcagct tttgcatcgg
     4441 caattttttt ctcgagcgcc tgtgccgtgc ggtgtgcgtc ttcacggtcg tgcgcaatca
     4501 gcatcgacgc gttcgaccca ttgcccagga ttgtatcgac gcggcgcatg aaatactgct
     4561 cgctacccgg gccctcagat accttgatcg atagtttttt gaggctgtat tcaaaccggt
     4621 ctttgctggc atagatgatg gtaccggcga tggcaatcgg tacgagcaca tagagaatcc
     4681 atgtaagcgg tttgcggttg cggtgaatca cctgtgccag cttgcgcgca ttgtctctct
     4741 ggatgaactt gtgtatcttc gagactttct gcggccgcca gcgctcgaat agcaccaaaa
     4801 agcagggaat aatcagcgtg agcgcagccc agcagagaac catgccgatg ccgccaataa
     4861 aaccaaactg tgaaaaaccg cggaaggtcg ttaccgacag tattgaatac gaaatcgaag
     4921 tagcgaaagc ggccatgaac gtggctgaaa tagtatagaa gatcgcccgg ttataagagc
     4981 gccggaacga agtgccgtgg ccgcgttctt ccaggtagcg ggccatgagt ataaggccaa
     5041 aattgatgcc gttaccgaca atgatggaag ccaggaaggc tgtctgctgg ttcaggtaac
     5101 caatacggaa ataggtgacc gcaaaggtca gcagaatacc taccagtact cctgcacaga
     5161 gcatgagcac catgcgcaga gagcggtagt aaagatagat agagcccaga acaagaaaaa
     5221 tggtgagtgc agctgtttcg acggtatcct ggatgatact cgcaaaattc tgcagcaggg
     5281 caacgtatgt gcccgcgagc ccaacttcca tctgtgggtg gaacttttta gggtcgagtt
     5341 tggcaatgtc tgcctgcagc cggctgacga ctgcctcgga aaatttctgg tccgcggcat
     5401 cgcccgttgg ccgaataatc atcgccacgt tctcaccggt cttgtccgtg aagtagccct
     5461 ctttattgaa gccaaacggg ttttcaccct tgtacttgtc gaagatcttc ttcaatacct
     5521 tcttgagctg cgcatcgggg ttactctctt cgaggtccat gccgaaggcg ctcagccgcg
     5581 tcttttcgag gcgtttgcgc agcgtactgc gcagctcttt cagctcttcg gtacccaggt
     5641 agtagaagag ccggtctttg aaaaactgct cggtatcgcg cgtgttgtaa tcaaagaact
     5701 tgacgagatc cttcgggtat ttttcgagca aagccgcata cgcctgcgca aatttcttat
     5761 tcgcctcaaa atcgggagaa tagatcgaaa caaaaatatt gccgaccccg ccggtgcgtt
     5821 cagtcgcttc tttgacatgc acgacgctgc ggtcgttttc gggcagcagc gtcgcgaagt
     5881 cggtgcgcac gtcctggaaa agctttacgg tgaagaagct cgcgacaatg gaaacagcga
     5941 g
//
LOCUS       DAOQJF010000204         5887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7708_length_5887_cov_5.743141, whole genome shotgun sequence.
ACCESSION   DAOQJF010000204 DAOQJF010000000
VERSION     DAOQJF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7708_length_5887_cov_5.743141"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLF85_11030"
     CDS             <1..315
                     /locus_tag="PLF85_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20244.1"
                     /translation="VGIVGKRIERLGSFQKAEIQPTIDFKKLDFVIVIQKKVETEALN
                     FLPLKPDTVEEPKLAPAPPARKGEADAADDVKPKAKVDKAATGTKPATAQPDAEPAEE
                     TP"
     gene            312..863
                     /gene="mreD"
                     /locus_tag="PLF85_11035"
     CDS             312..863
                     /gene="mreD"
                     /locus_tag="PLF85_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNE20245.1"
                     /translation="MILEKVVIGIAIFVSYFLQTSVDFFALGSIKPDFLLILTVYFAL
                     YRGAFSALWIGFFGGLLQDINLGGYTNLVKQDMQYYIGTSALAKTIIGFVAGKVARDI
                     NKEATSLIVIVVFVACLIKGIIMFFTVAIFHSSAHAASLITIVLPEALFTAILSIIWF
                     RLLRWAFPTVEKTEGQRIIRRIG"
     gene            867..2723
                     /gene="mrdA"
                     /locus_tag="PLF85_11040"
     CDS             867..2723
                     /gene="mrdA"
                     /locus_tag="PLF85_11040"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNE20246.1"
                     /translation="MAQTIQQFQLRQRLDRRVTGLMIFILGTLGILLLRLVDLQLIRG
                     EEFFEKSQRVIRRVVALTAPRGEMFARNYTSREKATYIVSNSSAVSLSVIPSHFPKKK
                     ELKDSVAKLERLLRFPEGSLTQKVADNKYKAHEEIILIENLKPEQTTVIADYYITFSK
                     FIIRPMSRRFYNLGASAAHLTGYIGLPNQREIGSGVRSYQYTGKNGLESQYDTILRGE
                     DGEIVQIKSATGDIEEQKVFRNFVPGNNLILTVDADLQVAAAKAMGDKTGGLIAIRPY
                     TGEILAIVSKPDFDPNILIALDNPERPAHIRTIQEQKAELNRAISTKYPPASTFKPLV
                     ALAGLEEKRLNKSDHYFCPGKFVLKSSYKGSPDAIFNDWARHGDNDLVGGIAQSCSVY
                     FYEAGYRMGAEPMIKYSRYFHLGEKSQIDLPSEIAGFVPDGAWKEKTFNTRWFDGDTI
                     NLSIGQGFIETTLVGMIGFYSAVATGGTIYKPHLIRQVRFAENDQVKFEIKPQVVDQI
                     PLSKSAVQTVREGLRAVTTRGTAASLFRLPGLIPVGGKTGTVQTRSGDRFNKASQHAW
                     FIGYGPFDGEPKDVLVVGVIVERGVGGAVGAAPIARDVFLKYSQKLKAGTID"
     gene            2723..4246
                     /locus_tag="PLF85_11045"
     CDS             2723..4246
                     /locus_tag="PLF85_11045"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNE20247.1"
                     /translation="MDRFARLDWVSIFLAVAATLVGVIALYGGGVTGEDLAQKKLFWL
                     ILGVIVMLVFANINYQLLGSFAPIIYAVGIFALLLVMVPFIGKEVKGARSWFSFLGVS
                     FQPAELMKLVIVITLSKYLVLRESEIGKLKELIIPSVLATVPALLIGVQPDLGGAVMF
                     MAILFGMLFIGGANTAVLFGFAIIGFFAVFTPMFLEYERYVIVDDIYNAIKDDHFRLA
                     DAVRILNFEVWEVAENLNYTTRLVQDRNLVTWAKETLAAGENLKIFWQAAAEIEKNNP
                     SFLRDMLRNTTVMVTIGATGIFFYGLGVLGNFMTGAHWMRRVANIMLIVGLSFASGFM
                     FNKFINFKPHQVIRIVSFVNPDKFQKGAGYQLRHSLITVGSGKFTGKGLFKGDMTKGD
                     TPFLPEWYNDFIFSVVGEQMGFWGVTGLLLLLFGLILRAINIAVTSKDDFGAMLASGI
                     ALIFFLHVFINIGISLGLLPVTGIPLSFVSYGGSHMLLCFICVGILINIYRRRFINA"
     gene            complement(4284..5180)
                     /locus_tag="PLF85_11050"
     CDS             complement(4284..5180)
                     /locus_tag="PLF85_11050"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNE20248.1"
                     /translation="MTKQFPSVFILPLLLATTALANCAREKSSAPLMHEKKSANLAEA
                     EEQSAGAATAPLAYRDDADRRTGHAEVTPFGPVFKPVASAERRLEYAVNLSYQISDLK
                     ATRAFFNQWIPRFGFLLSENASGLHGGYLSLQVKIRSGQLYQALAELDGIGTLANEQI
                     TVTDHTEAAVVRSLSAAREEFRLRRRNAAVQQGGSQSKNWQAAENLLAASEDKDLQNR
                     IEEWRINDRTAWATVNISMVMPVVAQSATIDVPHFRNAFVGVLNVLLQLLYLLIYLIP
                     VSILGWLGWRGFRRLRSAITGS"
     gene            complement(5254..>5887)
                     /locus_tag="PLF85_11055"
     CDS             complement(5254..>5887)
                     /locus_tag="PLF85_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNE20249.1"
                     /translation="GLVRGYDGGWHNLVRFGIAIDTRDYEPDPNKGQMFEAIVETSNK
                     AYGSQFNFTRYTVSEKFFYSPFEKLVDLVIAGRAAYTQAVGDVPFYSLDQFTSTERIY
                     QGGLGGLRSLRGYKASRFMATNMALANLELRWTMFDFTVAGQRFAPIFVPFFDIGSAF
                     DQPKDISTSVWRYAYGAGIRIAWNQATIIMVDYAMSKEDSNMFINFSHIF"
BASE COUNT         1282 a         1533 c         1628 g         1444 t
ORIGIN      
        1 gtggggatag tcggtaaacg tattgagcgg ctgggtagct tccagaaggc agaaattcag
       61 cccacgatcg acttcaagaa gctcgacttc gttattgtca ttcagaaaaa agtagaaacc
      121 gaggccttga atttcttacc gcttaaacca gacacggtcg aagagccgaa actggcgccc
      181 gctccacctg ctcgtaaggg cgaggctgac gcggcagacg acgtcaagcc taaggcaaag
      241 gtcgataagg cagctactgg aacgaaacca gcaacggctc agcctgacgc agagccagcg
      301 gaggagacgc catgatcctc gaaaaagttg tcatcggtat tgcaatcttt gtctcttatt
      361 ttttgcagac aagcgtcgac ttctttgcgc tcggcagtat caagcctgac tttcttctca
      421 tcctgacagt ctattttgcg ctctaccgcg gtgcattttc cgcactctgg attggcttct
      481 ttggcggcct gctgcaggat atcaacctcg gcggatacac caaccttgtg aagcaggaca
      541 tgcagtacta cataggtact tcggcgctgg caaagaccat tatcggcttt gtcgccggca
      601 aggtagcgcg tgacatcaat aaagaagcga cgtcgctgat cgtcatcgtg gtgtttgtcg
      661 cctgcctgat caagggtatc atcatgttct tcacggtggc gatttttcac agcagtgcgc
      721 acgctgcctc gctcattacc atcgtattgc ccgaagcgct ttttacggcg atcctgtcga
      781 ttatctggtt caggctgttg cgctgggctt tcccgaccgt cgaaaaaacc gaaggacagc
      841 gaattatccg ccgtattgga taggtaatgg ctcagactat acaacagttt cagctgcgcc
      901 agcgccttga ccgccgcgtt acaggtttga tgatttttat ccttggtact cttggcattc
      961 tgctgctgcg cctcgtcgac ctgcagctga tccgcggcga agaatttttt gaaaagtccc
     1021 agcgggttat tcgccgcgtg gttgcgctca ctgcaccgcg cggcgaaatg tttgcgcgca
     1081 actataccag ccgtgaaaag gcaacttaca ttgtctcgaa ctcctctgcg gtatcgttaa
     1141 gcgtcatccc ttcgcatttt ccgaagaaaa aagagctcaa ggattcggtg gcgaagctcg
     1201 agcggctgtt gcgctttcct gaaggctcac tcacgcagaa ggttgccgac aacaagtaca
     1261 aggcgcacga agaaattatc ctgatcgaga acctgaagcc cgagcagaca actgttatcg
     1321 cagactatta tattactttt tcaaagttta tcatcaggcc gatgtcgcgg cgcttttata
     1381 acctgggtgc ctccgcggcg cacctgacgg gttatatcgg tttgcccaac cagcgcgaga
     1441 ttggttcggg tgtgcgcagc taccagtata cgggcaagaa cggtctggaa tcacagtacg
     1501 acacgatcct gcgcggtgaa gacggcgaga tcgtgcagat taagtcagcg actggtgata
     1561 tcgaagaaca aaaggttttc cgcaattttg ttcctggcaa taacctgatc ctgacggtcg
     1621 atgcagacct gcaggttgct gccgcgaaag ccatgggcga taagaccggt ggcctgatcg
     1681 cgattcgacc ctataccggt gaaattctgg caatcgtttc caaaccggat tttgacccca
     1741 atattcttat tgcgcttgat aatccggagc gtcctgcgca catccgaacg attcaggaac
     1801 aaaaggcaga gctcaaccgg gccatttcga ccaagtaccc gccggcttcg accttcaagc
     1861 ctttggttgc actggccggg ctcgaagaaa agcgcctgaa caagtcagat cattatttct
     1921 gcccgggaaa atttgtcctg aagagcagtt acaagggctc gcccgacgca atttttaacg
     1981 actgggcgcg ccacggcgac aacgaccttg tcggtggcat cgcgcagagc tgctcggtct
     2041 atttctacga ggcgggctac cgcatgggtg ccgagccgat gatcaagtac tcgcgctatt
     2101 ttcatctcgg cgaaaagtca cagatcgatc tgccgagcga aatagcaggc ttcgtgcccg
     2161 atggcgcgtg gaaagagaag acattcaata cgcgctggtt tgacggcgac acgatcaacc
     2221 tttctatcgg gcagggtttt atcgaaacga ctctcgttgg catgatcggc ttctattctg
     2281 cagtcgcaac cggcggcacc atctacaagc cgcacctcat cagacaggtg cgctttgccg
     2341 agaacgacca ggtcaaattt gaaattaagc cgcaggtggt tgaccagata cctctgtcga
     2401 agtctgccgt gcagaccgtg cgtgagggcc tgcgggccgt gacgacgcgg ggtacagctg
     2461 cctcgctgtt ccgcctgcca ggattaatac ccgtcggcgg taaaaccggt acggtacaga
     2521 cacgttcggg cgatcgtttt aataaggcat cgcagcacgc ctggtttatc ggttacgggc
     2581 cttttgacgg cgaaccgaaa gatgtgctcg tcgtgggagt catcgttgag cgaggggtag
     2641 gtggcgcggt gggtgcggcg ccgatcgccc gtgacgtctt tctcaagtat tcgcagaaac
     2701 tcaaagcggg gaccatcgat taatggatag atttgcgcgt ctcgactggg tttcgatctt
     2761 tctcgcggta gcagcgacac tggtgggtgt tatagctctc tatggtggtg gcgtcaccgg
     2821 tgaagacctt gctcagaaaa aactgttctg gttgatcctg ggcgtcattg tcatgctggt
     2881 ctttgccaat atcaattacc agctgctggg gtcattcgcg ccgatcattt atgcggtcgg
     2941 cattttcgcc ctgctgctgg tgatggtacc gtttatcgga aaagaagtca agggcgcgcg
     3001 ttcctggttt agctttttgg gtgtcagctt ccagccggcg gagctcatga agctcgtgat
     3061 tgttatcacg ctgagcaagt atcttgtact acgtgaatcg gaaatcggca aactgaaaga
     3121 gctgatcatt ccgtctgttc ttgcgacggt gccagcgctg ctcatcggcg tccagcccga
     3181 cctgggcggc gcggtgatgt tcatggccat cctgtttggc atgctcttta tcggtggcgc
     3241 aaacacggct gtgctgttcg gttttgccat cattggcttt ttcgccgtgt ttacgccgat
     3301 gtttctcgaa tacgagcgct acgtgattgt tgatgatatc tataacgcca tcaaagacga
     3361 ccattttcgc ctcgcagacg cggtacgtat tctcaacttc gaagtctggg aggtcgccga
     3421 gaacctcaat tatacgacgc ggcttgtgca ggaccgcaac ctcgtgacgt gggccaaaga
     3481 gaccctcgcc gccggtgaaa acctgaagat attctggcag gcggcggcag aaattgagaa
     3541 gaacaatcct tcattcctgc gcgatatgtt acggaacaca acggtaatgg taacgatcgg
     3601 cgcgaccggt atcttctttt atggacttgg tgtgctgggt aatttcatga ctggcgcgca
     3661 ctggatgcgg cgcgtagcga atatcatgct gatcgtgggt ctctcgttcg cctcaggctt
     3721 catgttcaac aagttcatca actttaaacc gcaccaggtg attcgtatcg tatcatttgt
     3781 taatcccgac aagttccaga aaggggcagg ctaccagcta cgacactcgc tcatcaccgt
     3841 cggttcggga aaatttacgg gtaaggggct ttttaagggc gatatgacca agggtgatac
     3901 gccctttctg cctgaatggt acaatgactt tatcttttct gtggtgggtg agcagatggg
     3961 cttctggggc gtgaccgggt tgctcctgct cctgtttggc ttaatcttgc gcgccatcaa
     4021 catcgccgtc acatcgaaag atgacttcgg ggcgatgctt gcctcaggta ttgcgctaat
     4081 cttctttttg catgtcttta tcaatatcgg tatttcactt ggtcttttac cggtgacagg
     4141 tataccgctg agttttgtga gctatggtgg ctcgcacatg ttactctgct ttatctgtgt
     4201 cggtatacta atcaacattt accgcaggcg cttcatcaac gcctgagcag gctccgggaa
     4261 ttagcctgcg tctggctcct tttttaggag cctgttatgg ctgaacgcag cctgcggaat
     4321 ccgcgccagc ccagccagcc cagaatgctg accggtatga ggtagattag caggtagagt
     4381 aactgcagca gcacattgag aactcccaca aaggcattgc gaaaatgcgg tacgtcgatc
     4441 gtggcactct gtgcgacaac aggcattacc attgaaatat ttaccgtcgc ccacgcggta
     4501 cggtcattga tacgccactc ttcgatgcgg ttctgcaagt ccttgtcttc gctcgccgcc
     4561 agcaggtttt cggccgcctg ccagttcttc gattgcgatc caccctgctg gactgctgca
     4621 ttgcggcgac gcaggcggaa ctcttcgcgg gctgcgctca aactgcgaac cactgctgct
     4681 tcggtgtggt cggtcacggt aatctgttcg ttcgccagtg tgccgatacc atcgagttct
     4741 gcgagcgcct ggtaaagctg cccgctgcga atctttacct gcagtgaaag gtatccacca
     4801 tgtaggcctg acgcattctc gctgagcaga aaaccaaagc gcggaatcca ctggttgaaa
     4861 aaggcgcgcg tggccttgag gtcgctaatc tggtagctga gattcacggc gtactcgagc
     4921 cggcgttcag cactggcaac tggcttgaac acagggccaa agggagtgac ctcggcatgt
     4981 cccgtgcgtc ggtctgcatc atcgcgataa gccagtggcg ccgtcgcggc acctgcagac
     5041 tgttcttctg cctcggccag atttgcagat tttttttcgt gcatgagcgg tgcgctgctt
     5101 ttttcacgcg cgcagtttgc cagggcagtt gtggccagaa gcaagggtaa gataaaaacc
     5161 gatggaaatt gttttgtcat gctgcttgga cgccgcagca gacgataagg ttcgcatttt
     5221 ctgcgggccg tcggcccgca gaaaatagca ttatcaaaag atgtggctga agttgatgaa
     5281 catgtttgag tcttctttgc tcatagcata gtccaccatg atgatcgttg cctggttcca
     5341 ggcgatacgg atacctgcac cgtaagcata gcgccagacg ctggttgaaa tgtctttggg
     5401 ttggtcaaaa gcactaccga tgtcgaagaa cggcacgaag atcggtgcaa agcgctggcc
     5461 tgccacggtg aagtcaaaca tcgtccagcg gagctcgagg ttggcgagcg ccatgttggt
     5521 cgccatgaac cggcttgcct tgtaaccgcg tagcgagcgc aggccgccca gtccaccctg
     5581 gtaaatacgc tctgtgctgg tgaattggtc gagcgagtag aaaggaacgt ccccgacggc
     5641 ctgcgtgtaa gcagcacgac cggcaatgac taggtcgaca agcttttcaa atgggctgta
     5701 gaagaacttc tctgagaccg tatagcgggt gaagttgaac tgtgatccat acgccttgtt
     5761 tgaggtttcg acaatggcct cgaacatctg gcccttgttc gggtctggtt catagtcacg
     5821 cgtgtcgatg gcaataccga aacggaccag gttatgccag ccgccgtcat aaccgcgcac
     5881 caggcct
//
LOCUS       DAOQJF010000205         5877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7724_length_5877_cov_4.808657, whole genome shotgun sequence.
ACCESSION   DAOQJF010000205 DAOQJF010000000
VERSION     DAOQJF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7724_length_5877_cov_4.808657"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..3280
                     /locus_tag="PLF85_11060"
     CDS             89..3280
                     /locus_tag="PLF85_11060"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE20250.1"
                     /translation="MVGRLSLFIAVLLYSPTLFAAPIENARASLREDGQPMRLTGKWR
                     FVAADKPAVALAEFDDSQSPLLNVENEWRLQGVEHNQNAWFRLRLLVPASLAGKDLGL
                     IVPPVSIASEIYFNGRLMGSRGKIGLGGEILAPNSAFMLYQIPAASVKAGAENVVAIR
                     VGQYQRVGGLFGNNYLYAGEYTQARDHYMVLLLGIVILGSAFIILGLYHLILYFGRRI
                     ETEFLYFSLIAITSGMHQLGMNGIGYHLTESRTLNQILIHLVTAGLPVSLVGFATKFF
                     DLRAKWPERIFLYSGILVALVLVLQQVIPSLYGIQVIYILPFSMLMIMASLIVFTVLV
                     VKAFRLKRHGAMLVGSGVLLYMLTVAHDILAYLTLPVEYRLGNIGFLIPVFGMATALA
                     QKIQRMHREKELTQQQAIENLKKADVLKDEFLANTSHELRTPLNGIIGLAESLVNGAT
                     GPLRENTMENLELIVSSGRRLSSLVDDILDFSRMKNNELRLHIEPLAVEPQVSLVLQL
                     SEPLLQGKPVALKKEIPPDLPLLLGDHARLQQVLHNLVGNAIKFTAAGSVRVRAWQDG
                     ETEVAIAVEDTGIGIAAEDLGRIFNAFEQVDSADNRRFGGTGLGLTVTKRLVELQGGR
                     IEACSQAGQGSVFTVYFPIARGTATIREAVNPILSRFPASRPVLLNDDVLVEELQAAG
                     VPSHGKVLAIDDEQINLRILQNMLTLNGYECITASGGKEAIALLESMQDEELPDVVLL
                     DVMMPEMTGYEVCQRIREKYPVQTLPVIMLTAKREEKDIVKGFQSGANDYLTKPFYQD
                     ELTSRVNTLYMLKAAVRRDQQLLSLEKELEIARQMQASLLPKKTIEHLSYDISWFFRP
                     MLSVGGDLLDVQQSREGDLCFLIADVCGHGIGAAMVTSMVKLTFLMSHRLMHEPAAML
                     KQMNETLAMILGGTFVTAAVVHLSANGRQIRFARAGHPPLLHYEAQSGKVAHYQLPGK
                     PMGDFPGNSEFLEQSIAIAPGDMLMLYTDGVTEAENTSGEMYGDRLHAFLASQGGNAA
                     AQISQQLVSQLDKQIAPRTEHADDIAYVVIKVK"
     gene            3343..4695
                     /locus_tag="PLF85_11065"
     CDS             3343..4695
                     /locus_tag="PLF85_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNE20251.1"
                     /translation="MLYEKSTDNLIKNLRCYITDKPGYLGKLLTTIGMHGGNIGDLRL
                     IKRAGLNNLREIEIYVRDQQHMQDILHAVGELDGIQVDSVSDAVLDLHKGGKIAMHSR
                     VSIENAGDVRRIYTPGVAAVCRAIQKEPDLMYDYTYVRNSVAIVTNGTAILGLGDIGV
                     RAGMPVMEGKAVLFDKLVGISGIPILIDSKDIQTCIDTIVNIAPSFGAIKLEDFRAPE
                     CFAIEEALMQKLEMPIMHDDQHGTATVVLAALMNATRYVGDDLRALSVGIVGLGAAGN
                     GIARLLKAFGVQTIYGADINEDAKTRLEQIGGTPLALGELMQKAQVVVTTTGVPNLIK
                     PEMVRARQIILALSNPDPEIKPEAAIAAGAAFAADGRGVNNALAFPGIFKGALNARAS
                     RINNRMKIAAAQAIASLANSDRQELVPALLNPDVHQKVTEAVERAALESGAVRKLSEM
                     "
     gene            4747..4818
                     /locus_tag="PLF85_11070"
     tRNA            4747..4818
                     /locus_tag="PLF85_11070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4780..4782,aa:Arg,seq:ccg)
     gene            5094..>5877
                     /locus_tag="PLF85_11075"
     CDS             5094..>5877
                     /locus_tag="PLF85_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE20252.1"
                     /translation="MTQISGAQLLIRFLKGRGVTTIAGIPGGAALPIYDALHGSSITH
                     ILARHEQGAGFIAQGMARATGVPAVTLATSGPGATNLVTAVADAYADSIPLLAITGQV
                     PQHLIGTDAFQEVDACHLFEKITRKSFFARSAEELYEILPKAWLLMTQGRPGPVHVDI
                     PKDVQLQKVEFRPFRFIAPSAHTADAAAIELAARMIGASERPVFYIGGGIIASGAAEV
                     VTTLARDQGIPVVSSVMGLGAFPAKDELFLGMLGMHAAPYTNY"
BASE COUNT         1295 a         1563 c         1642 g         1377 t
ORIGIN      
        1 ttttcatttt catactcctc aatccgggta attgaggtga gtgtcaaatc atttgacgca
       61 tacagcttcg attctgagcg tactgaacat ggtagggcgc ctctcgcttt ttattgccgt
      121 actgctttat tcgcctacgc tttttgccgc gccgattgag aatgcgcggg cttctctgcg
      181 cgaagacggc cagcccatgc gcttgacagg caagtggcgc tttgtcgccg cagacaaacc
      241 tgcagtggct cttgctgaat tcgacgactc tcaaagcccg ctcctcaacg tcgagaacga
      301 atggcggctg cagggggtag agcacaacca gaatgcctgg tttcgcctgc ggcttttggt
      361 tccggcaagt cttgccggta aagatttggg actcattgtg cccccggttt cgatcgcttc
      421 agagatctat ttcaacggtc ggctcatggg cagcaggggt aaaattggtt tgggcggaga
      481 aatcctggct cccaattcgg cttttatgct gtaccagatt cccgcggcga gcgtaaaggc
      541 cggcgccgag aatgtggttg ctattcgcgt gggccagtac cagcgtgttg gcgggctatt
      601 cggaaataac tatctctatg cgggcgaata tacccaggcg cgagaccatt acatggtgct
      661 gctgctcggt atcgttattc tgggatcagc gtttatcatt cttggcttat atcacctgat
      721 actctatttc ggtcgccgta tcgaaaccga atttctctac ttcagcctga tcgcgattac
      781 ttcaggcatg caccagctgg gtatgaatgg catcggctac catctgaccg aaagccgtac
      841 cctcaaccag atactcatac acctggtgac tgccgggttg ccggtatcgc ttgtgggctt
      901 tgccacgaaa ttctttgacc tgcgcgcaaa atggccagag cggatttttc tttattccgg
      961 catcttggtc gcgctggtac tggtgctgca gcaggtgatt ccctcccttt acggcattca
     1021 ggtcatctac attcttccgt tctcgatgct gatgattatg gcatcgctca ttgtttttac
     1081 cgtgttggtc gtaaaagcgt tcaggctaaa gcgccatggc gccatgcttg tcggttcggg
     1141 ggttttactc tacatgctga cagtggcgca cgatattctc gcttacctga cattgcccgt
     1201 cgaataccgg ctggggaaca tcgggtttct gattccggtc tttgggatgg cgacagcgct
     1261 ggcgcagaag atacagcgga tgcaccgcga gaaagaactc acgcagcagc aggcgattga
     1321 gaatttgaaa aaagccgacg tgctaaaaga cgaattcctg gcgaatacat cgcacgagct
     1381 gcgcacaccg ctcaacggca tcattggcct cgccgagtcg ctggtaaatg gggccactgg
     1441 tcccctgcgc gagaacacga tggaaaacct tgagctcatc gtgagcagcg gtcgacgcct
     1501 gtcgtcactg gttgacgaca ttctggattt ttcgcggatg aagaacaatg aactacggct
     1561 gcacattgag ccattggcag ttgaaccgca ggtttcgctt gtgctacagc tttcggagcc
     1621 gttgttgcag ggaaagcctg tcgcattaaa aaaagaaatc ccacccgatt tacccctgct
     1681 gctgggcgat catgcgcgcc tgcagcaggt attgcacaac ctcgtcggca acgcaatcaa
     1741 gttcactgca gcgggcagtg ttagggtgcg tgcatggcaa gacggcgaga cagaagtagc
     1801 cattgccgtt gaagataccg gtatcggaat cgcggccgaa gacctggggc gaatcttcaa
     1861 tgcgtttgag caggtagatt ctgccgacaa ccgccgcttc ggtggcacag ggttaggact
     1921 tacggtcacc aagcgccttg tggagttgca gggcggtagg attgaagcct gttcacaggc
     1981 agggcagggt tcggttttca ctgtctattt tccaatagcg cggggcactg ctacaatccg
     2041 tgaagcggtc aacccaatcc tttcgagatt ccctgcgtcg cgtcctgtat tgctgaatga
     2101 tgatgtttta gtcgaagagc tccaggcagc tggtgtccct tcacatggta aggttctcgc
     2161 tatcgacgat gagcagatta acctccgtat cctgcaaaat atgctcacgt tgaatggtta
     2221 tgagtgcatt acagcctcag gcggcaaaga ggcgattgca ttgctcgaaa gcatgcaaga
     2281 cgaagaactg cccgatgttg tactgctcga tgtgatgatg ccagagatga ccgggtacga
     2341 agtttgccag cgcatacgcg aaaaataccc ggtgcagacg ctgcctgtta ttatgctcac
     2401 ggccaaacgt gaagagaaag atatcgttaa gggatttcaa agcggcgcca acgactacct
     2461 gacaaaaccg ttctatcagg atgaactaac gagtcgcgtt aatacactct acatgcttaa
     2521 ggcggcagtg cgccgcgatc aacagctgct ttcgctcgag aaagagctcg agattgcgcg
     2581 gcagatgcag gcatcccttt tacccaaaaa gacgattgag catttaagct atgatatcag
     2641 ctggttcttc aggcctatgc tttcggtagg cggagacctg cttgacgtgc agcaatcgcg
     2701 cgagggggat ctctgctttc tcatcgccga tgtctgcggg catggcattg gcgctgcgat
     2761 ggtgacttcg atggtcaaac tgacctttct catgtcgcac cggctgatgc acgaaccggc
     2821 ggcaatgttg aagcagatga atgagacgct ggcgatgatt ctcggcggaa cttttgtcac
     2881 cgctgcagtt gtgcatcttt cagccaacgg caggcagatc cgttttgccc gggcgggtca
     2941 tccaccgctg ttacattatg aggcgcaatc tggcaaggtg gcgcattacc agttgcccgg
     3001 caagcccatg ggggattttc ccggaaacag cgaattctta gagcaaagta ttgctattgc
     3061 gcccggcgac atgctaatgc tttataccga tggcgttaca gaagcggaaa atacctcggg
     3121 cgaaatgtat ggggaccgac tacacgcatt tcttgccagt caaggcggca acgcggcagc
     3181 gcagatcagc cagcagcttg tatcacagtt agacaaacaa attgctccca ggacagaaca
     3241 tgctgatgat atcgcctatg tggtgatcaa agtgaagtag tcgcatccct tcgctgaaat
     3301 ctattttcgc cctgttcaac atctgtttta catccttgcc tcatgctcta tgagaaatcc
     3361 acagacaacc tgataaagaa cctgcgttgc tacatcaccg ataagcccgg ttacctcggc
     3421 aaactgctca cgactatcgg tatgcatggc ggcaatatcg gtgaccttcg cctgatcaaa
     3481 cgcgcggggc tcaataacct gcgagaaatc gaaatttatg tgcgcgacca gcagcacatg
     3541 caggatattc tgcatgcggt gggagagctc gacggtattc aggtagactc tgtctctgac
     3601 gcggtacttg acctgcacaa gggtggtaag attgcgatgc acagccgggt ttctatcgag
     3661 aacgccggcg atgtccgccg catctacaca cctggcgtag ccgcagtttg ccgtgccatc
     3721 cagaaagagc ctgacctcat gtacgattac acgtatgtgc gcaactctgt ggcgattgtg
     3781 accaacggta ctgcgattct cggtttggga gatatcggtg tacgagcggg tatgcccgtg
     3841 atggagggca aggccgtgct gtttgataaa ctggtcggta tcagcggcat tcctatcctg
     3901 attgattcca aggacataca gacctgtatc gatacgattg tcaatatcgc gccttcattt
     3961 ggcgccatca agcttgaaga ttttcgtgca cccgaatgtt tcgcgatcga agaagctctc
     4021 atgcagaagc tcgagatgcc gatcatgcac gacgaccagc acggcaccgc taccgtggtg
     4081 ctggctgcgc tgatgaacgc cacgcgctac gttggtgatg acctgcgcgc cctgtcggtc
     4141 ggtattgtag gcctgggggc tgcaggcaac ggtatcgcgc gactgctcaa ggcattcggt
     4201 gtacagacca tctacggtgc cgacattaat gaagatgcaa aaacccgcct cgaacaaatc
     4261 ggtggaactc cactcgcttt gggtgagctg atgcagaagg cgcaggttgt cgtcacgact
     4321 accggtgtgc ccaacctcat caaacccgaa atggtacggg cgcgacagat cattcttgcg
     4381 ctttcaaacc ccgaccccga gatcaaaccc gaagcagcga ttgccgccgg cgcggcattc
     4441 gctgccgatg gccgcggagt caacaacgcg ctggcgtttc cgggtatctt taaaggcgca
     4501 ctcaatgccc gtgcgtcgcg catcaataac cgcatgaaga tcgcggctgc tcaggcaatc
     4561 gcctcgctgg ccaatagcga ccggcaagag ctcgtgccag cccttttaaa ccccgatgtg
     4621 caccagaagg tcaccgaagc cgtcgagcgc gccgcactcg aatcgggtgc ggtaagaaag
     4681 ctcagcgaaa tgtaatgaca gcgtgtttcg cgcatgcgct ttgcgcatgc gcgaaacacg
     4741 caaaaagccc tcgtagctca gtggatagag cgttggtctc cgaagccatg tgcacaggtt
     4801 cgattcctgt cgagggcgca tggatgcgcc cagttttgcg gccggcacga tgccgtcttt
     4861 gccgcattcg agggcgcagt gaattttgct ttacgggagc agcgaattcg ctgctcccgc
     4921 aaagcaagcc ctatggttaa caaaccgcaa tcactctggc tagtctctgt cgtggtggtg
     4981 gtcgtggtgt gttctgccgc tggggcatag attttatcta tccccgcggc tttcagccag
     5041 cggggatccc caaacactcc gccagctgaa ggccctcgga taaagagggt acaatgacac
     5101 agatcagcgg agctcagctc cttattcggt ttttgaaagg acgtggagta accacgattg
     5161 cgggaattcc cggcggcgct gcactgccaa tttacgatgc gctgcatggc agcagtataa
     5221 cgcacattct tgcgcggcat gaacagggtg ccggatttat tgcgcagggt atggcgcggg
     5281 ctacaggggt tccggcagtc acgctggcca cttctggccc gggtgcgacg aacctggtaa
     5341 cggccgtcgc cgatgcatac gccgactcga ttccactgct ggcgatcacc gggcaggtgc
     5401 cgcagcacct catcggtact gatgcattcc aggaagtcga tgcctgccac ctgttcgaaa
     5461 agattaccag aaaatctttc tttgcccggt ctgcagaaga gctctatgag attttgccca
     5521 aagcctggct gctgatgacg caggggaggc ccgggccggt acacgttgat attcctaaag
     5581 acgttcaatt gcagaaggtg gagttccggc cgttcagatt cattgcccca tctgcgcata
     5641 cggccgatgc tgctgcgatt gagcttgccg cgcgcatgat tggtgccagc gagcgcccgg
     5701 ttttctacat tggcggtggc atcattgctt caggcgctgc ggaagtcgtc acaacactcg
     5761 cgcgcgatca aggcattccc gttgtttcga gcgttatggg tttaggggcc tttccggcta
     5821 aagatgaact ctttctcggt atgctcggca tgcacgcagc gccgtacacg aattata
//
LOCUS       DAOQJF010000206         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7789_length_5851_cov_4.177191, whole genome shotgun sequence.
ACCESSION   DAOQJF010000206 DAOQJF010000000
VERSION     DAOQJF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7789_length_5851_cov_4.177191"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..877)
                     /locus_tag="PLF85_11080"
     CDS             complement(224..877)
                     /locus_tag="PLF85_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HNE20253.1"
                     /translation="MLDKTGIAKRIAREVKDGYYVNLGIGIPTLVSNYIPEGMNVVLQ
                     SENGLLGMGPFPYEGEEDPDYINAGKQTITTLPGSSIFDSAMSFGMIRARKVDLTILG
                     AMEVAENGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKANESKLLKRCTLP
                     LTGVRCVKKIVTELAVIDVTPQGFRLLETAPGVSVEQVKNATEGHLVVDGQVPEMQI"
     gene            complement(881..1588)
                     /locus_tag="PLF85_11085"
     CDS             complement(881..1588)
                     /locus_tag="PLF85_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNE20254.1"
                     /translation="MNKTVSNAEAAIADIPNGAVLGIGGFGLCGIPENSIAALVHKGV
                     KDLTCISNNAGVDNFGIGLLLKTRQVKKMIASYVGENAEFEKQLLSGELDVELIPQGT
                     LATRCLAAGYGMPAIFTPAGIGTEVAEGKETRKFMIDGAYKEFLMEYAFQIDFALVKA
                     WKGDTHGNLVFRGTARNFNPLMAMAGKTTIAEVEELVPAGTLDPNYIHTPGIYVHRIF
                     QGNNYEKRIEQRTTRKR"
     gene            complement(1641..2546)
                     /locus_tag="PLF85_11090"
     CDS             complement(1641..2546)
                     /locus_tag="PLF85_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE20255.1"
                     /translation="MPLRSHNILALLTQIGHDLAVEKAVEESPAFLQLVGTESSWADI
                     FSHIDSISDFSLRHKLRSLEFDYTVAHVRNQMAHVLPRSGNDIWALERTSYLTGLFSD
                     AFTLWDEYCAALDMLEVDLREELESRELTPRRLKNRMEDLETVRRVIAAINTVLFEKM
                     GLHGEEGAINRVESHSAPLVLCERVPGIPLSLAIIYLIAGNRLGFPLYGVNTPGRFLI
                     KWQLGELELFIDVFDKGSLITRQNLEMLIAANLSTQSTRLLEAAPFEVITRRSLANLI
                     ALSLRAEDNDRARKLEALSRGLFGF"
     gene            complement(2546..2872)
                     /locus_tag="PLF85_11095"
     CDS             complement(2546..2872)
                     /locus_tag="PLF85_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20256.1"
                     /translation="MQIGEILLYIVLPTAVLTSLVTALLVYYLARKLYYDEIEKPIDD
                     LPDRIALKVEEAGDRLLPRFEERVKKGFLEALKESPKLGPEMTVGVIESTLKSMDQIL
                     TGRRDR"
     gene            2965..3330
                     /locus_tag="PLF85_11100"
     CDS             2965..3330
                     /locus_tag="PLF85_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20257.1"
                     /translation="MKPRLLLVLLLVLLAACASRKNRVYPDNGVLTYLDASAGNVGGN
                     LDSTGVQMHYRGPLKDSIEDIQLLEASAAWRKGGRKAALRELQEKAQKLNADGLYDIQ
                     FADDSSGTEASGIAFRFRR"
     gene            3334..3699
                     /locus_tag="PLF85_11105"
     CDS             3334..3699
                     /locus_tag="PLF85_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20258.1"
                     /translation="MIALLRVTLFFALPALVLGYASPRYSPLHYGVREIAVLVFFFLI
                     AIFLMRKVGPFSLAQFLVLLSVAVSQTAVEYLLQRYGYSQKFAQSLWELPANYDGILA
                     AAWLPLAVRSAAAFLASRF"
     gene            3794..4573
                     /locus_tag="PLF85_11110"
     CDS             3794..4573
                     /locus_tag="PLF85_11110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE20259.1"
                     /translation="MKLASVEYAPLRQKLSGEVLESLDRYGDLLFEQAMPAGFIGVCT
                     REELTLRHVFDSILPAVAPESSGLIPLWQDARLNVFDLGAGAGLPSLPLAILFPQHHF
                     HLVDAQEKRLAFGRAAAVALGLKNVSFYHCVVQDFEKKFPKAAKADVVVFRAFRKILA
                     SLELALHVLRDAPAPRGGEVGHAQPKVLYWRSQPVPFSAEGTKRVSDLGYETEQFMKF
                     ASAENVLPRGMYFFTHARAAVKPFPRQWKKISADRLVEVES"
     gene            4577..5344
                     /locus_tag="PLF85_11115"
     CDS             4577..5344
                     /locus_tag="PLF85_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE20260.1"
                     /translation="MKTIALANQKGGVGKTTTTINLGAYLANEGQRVLIIDIDPQGNA
                     GSGLGLDITQAKSTIYHWLIDPSAPLPAQPTNQKNLEIIPANIDLSGFEIDVREDNNR
                     DYILKSRLAQISDRYDFVLIDCPPSLGILTINALTAANSVLIPLQCEYFALEGLTQLL
                     RIINLVRTKLNPQLVIEGVLLTMFDARTKLASQVVQDVREHFRKQVYDVIIPRNVKLS
                     EAPSFGQPISVYDPQSTGAIAYKTLAKDLLARQGATA"
     gene            5341..>5851
                     /locus_tag="PLF85_11120"
     CDS             5341..>5851
                     /locus_tag="PLF85_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE20261.1"
                     /translation="MSKQQGKVLGRGLSNLLDSTVVAEAGERVQEIPVDKIRPNPENP
                     RKKFERTAIEELAQTIKKHGLLQPILVQKKDDHYIVISGERRLRSLMHLGMAKAPCIV
                     KELDRRKTLEVSLIENIQREQLDAIEEANVYKLLLADYSLTQEALAEQVGKNRATIAN
                     RLRLPNLPIE"
BASE COUNT         1225 a         1649 c         1634 g         1343 t
ORIGIN      
        1 ctttatcgaa attccattca gttaaatcaa gaacaccgtt cactgctctc ggtgcctgct
       61 tccattcttt attgcacgat atgagaacga aaagtaatgg tacgagtata ttgattctgg
      121 gcatgattct acccagagca aaaacaaagc tatatcctga tgaatagctg attatcgggc
      181 accatacccc caaaacaggg ggtatgacag gtgcctcagc gagctagatc tgcatctcgg
      241 gtacttgccc gtcgacaacc aggtggcctt ctgttgcatt cttcacctgc tcgacactca
      301 cacccggtgc agtctcgagc agtctgaaac cctgcggtgt cacgtcgatg accgccagtt
      361 cggtgacgat cttcttcacg cagcgaaccc cggtcagggg cagggtgcag cgtttgagca
      421 gcttcgattc atttgccttg ttcacatgct gcatcgcgac gatgatgttc ttggccgatg
      481 ccacgagatc catggcgccg cccatgccct ttaccatctt gcccggtatt ttccagtttg
      541 cgatgtcgcc gttttccgca acttccatcg cgcccaaaat tgtcagatcg actttgcgtg
      601 cgcgtatcat gccaaagctc atcgccgagt cgaagatcga tgaaccgggc agggtggtga
      661 tggtctgttt gccggcgttg atgtagtcag ggtcttcttc accttcgtaa gggaaggggc
      721 ccatgcccaa aagtccgttt tctgactgca ggacgacatt cattccttcg ggtatataat
      781 tcgaaaccag tgtgggaatt ccaatgccga ggttgacgta gtagccgtct ttgacctcgc
      841 gtgcgatgcg ttttgcaata cctgttttat cgagcataaa ttacctcttc cgggttgttc
      901 tctgttctat gcgcttctcg taattattac cctggaagat tctgtgcaca tagataccgg
      961 gtgtatggat gtagttgggg tcgagcgtgc ccgcgggcac cagctcctcg acttctgcaa
     1021 tggtggtttt gcccgccatc gccatcagtg ggttaaaatt gcgtgccgta ccgcggaata
     1081 ccaggttgcc gtgcgtgtcg cctttccagg ctttcaccag cgcaaaatcg atctggaagg
     1141 cgtattccat gaggaattct ttatatgcgc catcgatcat gaacttgcgg gtttcttttc
     1201 cttcggcgac ctcggtgcca atgcctgcgg gcgtgaagat tgccggcatg ccgtacccgg
     1261 cggcgaggca acgcgtcgcg agggtaccct gcggaatcag ttcgacgtcg agttcacccg
     1321 agagcagctg tttttcaaac tcggcatttt cgccgacgta agaggcgatc atctttttta
     1381 cctggcgggt cttgagcaag agaccaatgc cgaagttatc gacacccgcg ttgttcgaga
     1441 tgcaggtgag atctttcacg cccttgtgca cgagcgcggc gatggaattt tcgggaatac
     1501 cgcagagccc gaagcccccg atacccagca cggcgccgtt cggtatatcg gcgatcgctg
     1561 cctctgcatt gctgactgtt ttattcatcc tggtagaatc tcctcttttg ataggccggt
     1621 tttttaagca gtgtgtttta tcagaacccg aacaaccccc gcgacaaggc ctcgagcttg
     1681 cgtgcccggt cattgtcttc ggcgcgcagc gacagggcta tcaggtttgc cagtgatcgg
     1741 cgggtaatca cttcaaaggg agctgcctcg agcaggcgcg ttgactgcgt gctcaggttg
     1801 gccgcgatca gcatctcgag attttgccgt gtaatcagcg aacctttgtc aaagacatcg
     1861 ataaagagct ccagttcgcc cagctgccat ttgatcagga agcgcccggg cgtatttacc
     1921 ccgtagagcg ggaaacccag ccggttaccg gcaatcaggt agataatcgc gagcgagagc
     1981 ggaataccgg gcacgcgttc gcacagcacc agcggtgccg agtgcgactc gacgcgattg
     2041 atcgctcctt cttcgccgtg cagccccatc ttttcaaaaa gcacagtgtt gatagcagct
     2101 atcacgcgcc gcacggtctc gagatcttcc atgcggttct tgaggcggcg cggtgtcagc
     2161 tcgcggcttt caagttcttc gcgcaggtct acctcgagca tatcgagcgc cgcgcagtat
     2221 tcatcccaca gggtgaacgc gtcagagaag agtccggtca ggtaactggt gcgctcgagg
     2281 gcccagatat cgttgcccga gcggggcaac acatgggcca tctggttgcg cacgtgggca
     2341 acagtgtaat caaactcgag cgagcgcagt ttgtggcgca ggctgaagtc tgagatcgaa
     2401 tcgatgtgcg aaaaaatgtc ggcccaggaa ctttcggtac ccacaagctg caaaaacgcc
     2461 ggcgattctt cgacggcctt ttccacagcc aggtcatgcc caatctgcgt cagcaatgcg
     2521 agtatgttgt ggctgcgcag cggcattacc ggtcgcgtct tcctgtcaga atctggtcca
     2581 tacttttgag agtgctttca atcaccccga ccgtcatttc ggggcccagc ttgggggact
     2641 ctttcagggc ttcgagaaac cccttcttga cgcgttcttc aaagcgaggc agcaggcgat
     2701 cccccgcttc ttcaaccttg agtgcgatac ggtctggcag gtcgtcgatc ggcttctcaa
     2761 tctcatcgta atagagtttg cgcgcgaggt aatagacgag cagggcggtg acgagcgagg
     2821 ttaagaccgc ggtgggcagc actatataaa gaagaatctc cccgatttgc atgtttctca
     2881 aggtatcaca tcccatcagg ccgaaaagcc cttttacggc ttgcccgctg aaagcctttt
     2941 gtgcttagct accacagagg cttcatgaaa ccccgacttc tgcttgtcct gcttttggtt
     3001 ctgctcgctg cctgcgcgag ccgcaagaac cgcgtctatc cagacaatgg cgtactcact
     3061 tatctcgatg cgagtgccgg caacgtcggt ggcaatctcg actccaccgg tgtgcagatg
     3121 cactaccgtg gtccgctcaa ggacagcatt gaagacatcc agctgctcga agcctctgct
     3181 gcctggcgca agggcggtcg aaaagcagcg ctgcgcgaac tgcaggaaaa agcgcagaag
     3241 ctgaacgccg atggcctgta cgatatccag tttgccgacg acagtagcgg caccgaagcg
     3301 agcggaatcg ctttccggtt caggcggtga catatgattg ctttgctgcg ggtgacgctg
     3361 ttcttcgctt tacccgcgct ggtgctgggc tatgcgtcgc cacgctactc gccgctgcat
     3421 tacggcgtac gcgaaatcgc ggtgctcgta ttcttttttc ttatcgcgat ttttctcatg
     3481 cgtaaggtcg gcccgttctc actcgcgcag tttcttgtac ttctgtcggt cgccgtgtcg
     3541 cagaccgcgg tcgaatacct gctgcagcgg tacggttact cgcagaaatt tgcgcagtcc
     3601 ttgtgggagc ttccggcgaa ctatgacggc atactggcgg cggcctggct tcccctggct
     3661 gtgcgctctg cagccgcgtt tctcgcgtcg cgcttttaag gccagcgccg cgggtcacag
     3721 ccgtgacctg ccgcgctgac accgatcaga gcgcgttgct ttacggcgcg tcctgatttc
     3781 gggccctgag cgggtgaagc tcgcctcagt cgaatatgct cccctcaggc aaaagctgtc
     3841 gggtgaagtg ctcgaatcac tcgaccgcta cggcgacctg ctgttcgagc aggcaatgcc
     3901 ggctggtttt atcggtgttt gcacgcgcga agagctgaca ctccgccacg tgtttgattc
     3961 cattctgcca gcggttgctc cggaaagcag cgggctcatt ccgctctggc aagatgcccg
     4021 gctgaacgtg tttgacctcg gggccggcgc ggggctgccg tcgttacccc tcgcgattct
     4081 ttttccgcag caccactttc acctcgtcga tgcacaggaa aagcgcctgg cttttggccg
     4141 ggcggcagcg gttgcgctcg ggctgaagaa cgtcagcttc taccactgcg ttgtgcagga
     4201 tttcgagaag aagttcccga aagcggcaaa ggctgatgtg gtcgtgttcc gcgcattccg
     4261 caaaatactc gcttcgctcg aattggcgct gcatgtgctg cgcgacgcac ccgcaccccg
     4321 ggggggagag gtcggtcacg ctcaacctaa agttctttac tggcggtctc agccggtgcc
     4381 tttttctgcc gaaggtacga agcgcgtgag cgacctggga tacgaaaccg aacaattcat
     4441 gaaatttgcc tctgcggaaa atgttctgcc gagggggatg tattttttta cacacgcaag
     4501 agcagcagtg aaacctttcc cgcggcagtg gaagaaaata tctgccgatc gtcttgtcga
     4561 ggtagagtct taagccatga aaacgatagc actggcgaac cagaagggcg gcgtgggtaa
     4621 gaccacgacc acgatcaacc tgggggctta tctcgcgaat gagggccagc gcgtgctgat
     4681 tattgacatt gacccgcagg gcaatgcggg cagcggcctc ggtcttgata ttacacaggc
     4741 aaagtcgacg atctaccact ggttgattga cccttcagcg ccgctgcccg cacagccaac
     4801 gaaccaaaag aatctggaaa tcatacctgc gaacatcgat ctttcgggtt tcgagatcga
     4861 tgtgcgcgaa gacaacaacc gcgactacat tctcaagagc cggctggcgc agatcagcga
     4921 ccgctatgat tttgtgctga tcgattgccc accgagcctc ggtattctca cgatcaatgc
     4981 gctcaccgca gccaactcgg tgctgattcc gctgcagtgc gaatacttcg cgctcgaagg
     5041 actcacgcag ctgctgcgta tcatcaacct ggttcgcaca aagctgaatc cgcagctcgt
     5101 gatcgaaggt gtgctgctga caatgtttga tgcgcgcacc aagctggctt cgcaggtagt
     5161 gcaggatgtc cgcgagcatt tccgcaaaca ggtctatgac gtcatcattc cgcgcaacgt
     5221 gaaactctct gaggcacctt cgtttgggca gccaatcagc gtctatgacc cgcaatcgac
     5281 aggcgcgatt gcctacaaga cgctggcgaa agatcttctc gcgcggcagg gagcaacagc
     5341 atgagtaagc agcagggcaa ggtactgggc agggggcttt cgaacctgct cgacagcacg
     5401 gtggtagccg aagcaggcga acgcgtccag gagataccgg tcgacaaaat tcgcccgaac
     5461 cccgaaaatc cccgcaagaa atttgagcgc actgctatcg aagaactggc gcagaccatc
     5521 aagaaacacg gcctcttgca gccgattctg gtacagaaga aagacgatca ttacatcgtg
     5581 atttcggggg agcgtcgcct gcgttctctc atgcacctcg gcatggcgaa agcgccgtgc
     5641 atcgtgaaag aactcgatcg ccgcaagacg ctcgaagttt cgcttattga aaacatacag
     5701 cgcgaacagc tcgacgcgat tgaagaggcg aatgtctata agctgttgct ggccgattat
     5761 tcgctgacgc aggaagccct cgccgaacag gtgggcaaaa accgcgccac gattgccaac
     5821 cgcctgcgtc tgccgaacct gccgattgaa g
//
LOCUS       DAOQJF010000207         5832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7827_length_5832_cov_4.815994, whole genome shotgun sequence.
ACCESSION   DAOQJF010000207 DAOQJF010000000
VERSION     DAOQJF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7827_length_5832_cov_4.815994"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLF85_11125"
     CDS             <1..689
                     /locus_tag="PLF85_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20262.1"
                     /translation="LFGAVEELAARVVKEAKSVLPNKGDAGKQQFDDGDIGPHELTIA
                     GGMAVYSLPTAFTGNYTGSSVLYAKDFKDAVAGSVAYTYRDFLFRNAQIELLAGGQFG
                     SNDLSVANDTKRIKASLTVLAAGGHLGYRFGFWRFFLTPFAGGGFSLASVKLDYSTLT
                     KLPLDAFGNELSSSNLNISAPFVEGGLHLGIQMTSRVALQFTGNYRQSIYLTESNGQL
                     MALGGLNFRF"
     gene            947..4318
                     /locus_tag="PLF85_11130"
     CDS             947..4318
                     /locus_tag="PLF85_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20263.1"
                     /translation="MKAATAAEIDTIAEVDAIPAGADLLQNYTNNDTTQDVTALTAGA
                     TYFFAVVVKDIAGNKSLYAPMSQTMAAPDTTPPVVGAAISFTSVSDTSMTVNWGAATD
                     AVTVQTGLQYRLVKATAIAAIDTIAEVDAIAGGDLLQDYNANDVTQSVAGLAASTTYF
                     FAVVVRDVAGNKAIYAPVSQTTNAAADVTPPNIGTAISFASITATTLTVNWGHAADAI
                     TAQAALEYRLVRATTSAAIDTIAEVDAISGGDLLQNYTANDITQNVSGLVEGSTWFYA
                     VVVRDVAGNKALYAPASQATTSAPPTVGTAISFASVTETSFTVNWGAATDAGTAQANL
                     EYRLVTAATSAAIDTVAEVDAISGGALLQDYTANDITQNVTGLTAGTTYFFAVVVRDA
                     VGNKTLYTPASQTTADTTAPTVGTAISFSSVAGTTMTVNWGAATDAVTPQANLQYRLV
                     KATTAAAIDTIAEVDAISGGDLLQNYTANDITQNVTGLSGSTTYYFAVVVRDTTGNKT
                     IYTPASQMTLDINAPTVGTAISFTSVVETSLTVNWGAATDTVTPQANLEYRLVKATTS
                     TAIDTIGEVEAITGGDLLQDYTANDIAQNVTGLSAGTIYFFAAVVRDAAGNKALYTPA
                     SQATPDLTPPTIGTAISFSSITGTSMAVNWGAATDAVTSQASLQYRLVKSTTSAAIDT
                     ITEVDAISGADLLQNYTANDVTQNVTGLTGSTTYYFAVVVRDAAGNKSLYAVASQETG
                     ETVPPAIGAAISLSNMTDTTLTVTWGAATDAVTPQVNLEYRLVQATSSTAIDTIAEVD
                     AISGGNLLQDYTANDLTQSVTGLAAGSTYFFAVVVRDQANNKAIYTPRMRYSWTNQTL
                     PSSSQWRSVTFGNGVFVAVASGPSTTAATSPDGKTWTARTLPSSQNWNSVTFANGIFI
                     ALAGGTNAATSPDGITWTMRTMSSFGSWQSVAFGNGVYSAIDYGSTAASTSPDGITWT
                     QQTVPTSANWQSVAFGNGAFTAIAYGSNIAATSPDGITWTMRTLPSSVNWKEVTFANG
                     IFVAVASNTSTAATSPDGINWTARTMSNFYTNSVTYGNGVFVSVSASAQGGSTSPDGV
                     TWTDTVGAFSGTSVAFGNGVFVSVEINSTNAATLP"
     gene            4454..5107
                     /locus_tag="PLF85_11135"
     CDS             4454..5107
                     /locus_tag="PLF85_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20264.1"
                     /translation="MILDQVCDRIGAISSAGWPISSWLPNANGTVYTIAISGSTVYAG
                     GNFTSIGGALRTRIAALDSSGIALAAFGTSANGIVRSIVPAGSTLYVGGDFSIIGGGN
                     RNRIGALDSTTGAGTSWVPEANGSVYSLAIVGTTVYAAGNFTTIGGQSRSRIAALDMT
                     INTNNATSWNPSASGLVWSLSASGTTVNMGGAFSTVGGFGISNIAAIDTTTSSVLEY"
     gene            5110..>5832
                     /locus_tag="PLF85_11140"
     CDS             5110..>5832
                     /locus_tag="PLF85_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20265.1"
                     /translation="MKLAAFFTLILSTATLHAQALKRVMILDFKNLDKNPDYTYLEES
                     ITEAVRNDLKAKFDFREMQKSDWQKLAEKNLHLWPEDNYTRSFGLHIGAVARQDIVVG
                     GYYQAIIEKKPRKGVRAHDFVIRAHVFVIDIGQRKVVSEFDMIMPADASLFTSIEVLA
                     ARVVAESKAVLPNKGELSQEVIDADELSPHEIGIFGGMSVLSVPAAFNGTFTTDKKLH
                     NKDVPQTLGVYGSFRYHDFIWPR"
BASE COUNT         1487 a         1498 c         1522 g         1325 t
ORIGIN      
        1 cgctcttcgg tgcggtcgaa gaactcgccg cgcgcgtggt gaaagaggcg aagtcggttc
       61 tgcctaataa gggcgatgcc ggcaagcagc agtttgacga cggcgacatc gggccgcatg
      121 aactgaccat tgcaggcggc atggccgtct attcgctgcc cacggcgttt acaggcaatt
      181 atacgggttc gagcgttctt tatgcaaaag attttaagga tgcagtagcg gggagcgttg
      241 cctacaccta tcgcgatttt ctttttcgaa acgctcagat tgaacttctg gcaggtggac
      301 aatttggttc aaacgatctc agcgtcgcga atgatacgaa gcgcataaaa gcttcattaa
      361 ctgttcttgc tgcaggcggt catttgggtt accgcttcgg cttctggcgt ttttttctga
      421 caccctttgc tggtggtggt ttttctctcg ccagcgttaa gctcgactat tcgacgctga
      481 caaaactgcc gctcgatgcg tttggcaacg aactttcatc ttcgaatctc aacatcagtg
      541 cgcccttcgt ggaaggcggc ctgcatctgg gcattcaaat gaccagcagg gttgcactgc
      601 agttcacagg taattaccgg caaagcattt atcttactga atcaaatggt caactaatgg
      661 cattgggcgg gttaaacttc agattctgaa gcatggagat tttatgaaca agcaaataat
      721 tcttacaata atggtcgcaa tctcgacctt atggcattgt cgaaacccag agacgcccgg
      781 cagtcttacc tttcgcgaag gcagcagcgc agctggcgct gacgtcaccc cgccggtcgt
      841 gggtacacaa gtcgccttta gcaatgtctc ggacacaacc ttaaccgtca gctggggctc
      901 tgccacagac attgtgacac ccacagtaga cctgcagtat aaactggtga aggctgcaac
      961 ggccgccgaa attgatacaa ttgctgaagt cgacgccata cctgccgggg ctgatctgct
     1021 gcaaaattat accaataacg atacgacgca agatgtgaca gccctgacag ccggcgccac
     1081 atattttttt gccgttgtag tcaaagatat tgcaggtaat aaatctttgt acgcgcccat
     1141 gagtcaaacg atggcggcac cagatacgac gccaccggtt gtgggtgcag ccatctcatt
     1201 tacgagcgtc tccgacacga gcatgacggt taactggggt gccgccaccg atgccgtaac
     1261 ggtgcagaca gggctacagt atcggctcgt gaaagccact gccattgcgg cgatcgacac
     1321 catcgccgag gtcgatgcca ttgcaggcgg tgacttgttg caggattaca acgcgaacga
     1381 tgtaacgcaa agcgtagccg gtcttgctgc gagcacgacg tatttttttg cggtagtggt
     1441 tcgggatgtc gcgggcaata aagcaattta tgccccggta agtcagacga cgaacgcagc
     1501 agccgatgtc acacccccaa atattggcac agccatatct tttgccagta taaccgcgac
     1561 gacgttaaca gtgaactggg gacatgccgc tgatgcaata acagcgcaag cggcgttaga
     1621 atacagactt gtcagagcaa caaccagtgc tgcaatcgat acgattgccg aagtagatgc
     1681 aatttcgggt ggcgatctac tgcaaaatta cactgccaat gatattaccc agaacgtatc
     1741 tggattagtc gaaggcagta cctggtttta tgcggtggtc gtgcgcgacg ttgcgggtaa
     1801 taaggcactt tatgcccctg caagccaagc aacgacatcg gcacccccga cagttggcac
     1861 ggcaatttca ttcgcgagcg tcacagaaac gagtttcact gtgaactggg gcgccgccac
     1921 tgatgcgggt actgcgcaag ctaatttaga ataccgactc gtaacagccg cgaccagcgc
     1981 agccattgac acggtcgctg aggtggatgc aatttctggt ggcgcattac tgcaggatta
     2041 cacagctaat gatattacac agaatgtgac ggggttaaca gccgggacta cgtatttctt
     2101 cgccgtagtc gtgagagacg ctgtgggtaa taagacccta tacactcctg caagccagac
     2161 aacagcggat accacggccc ctacagtcgg tactgccatc tctttttcca gcgtagcagg
     2221 tacaacgatg acagtaaatt ggggggctgc caccgatgct gtaacccccc aagctaatct
     2281 tcagtacagg ctcgtcaaag cgacaacggc ggcggcgatc gacacgattg ctgaagtcga
     2341 tgccatttca gggggtgacc tgttgcaaaa ctatacagcc aatgatatca cgcaaaatgt
     2401 gaccggcctg tcgggcagca ccacatacta ttttgccgtc gtcgtgcggg acacgacagg
     2461 aaacaagaca atctataccc cagcgagtca aatgactctg gatatcaatg ccccgaccgt
     2521 aggaaccgcg atatcattta ccagtgtagt tgaaacaagc ttgacggtaa attggggagc
     2581 ggcgactgac acggtgacac ctcaagccaa cctggaatac agacttgtga aggccactac
     2641 gagtaccgcg attgatacaa taggtgaagt cgaggcaatc acagggggcg atcttttaca
     2701 ggactatact gcgaatgaca tcgcgcagaa tgtgactggc ctgagcgccg gtactatcta
     2761 tttctttgcc gccgttgtca gggatgccgc tggcaataag gctctataca cacctgcgag
     2821 tcaggcgaca ccagatctga cgccgccgac aattggcacg gcaatttctt tttcgagtat
     2881 tacaggcaca tcgatggcgg taaattgggg ggctgctacg gatgcagtga catctcaggc
     2941 gagtctgcaa taccgactcg tcaagagcac caccagtgca gcgattgaca ccatcaccga
     3001 agtcgacgcc atatcaggag ctgatttatt gcagaattat acggcaaatg atgttacgca
     3061 gaatgttaca ggtttaacag gcagcacaac gtactatttt gctgtagttg ttcgagatgc
     3121 ggctggcaat aaatcactct acgccgtagc cagtcaggag acgggggaga cagttccgcc
     3181 agccatcggc gctgcaattt ctctttcgaa tatgactgac acgactttaa cagtaacttg
     3241 gggtgccgcg actgatgctg tcacacctca agtcaattta gaataccggc tcgtacaagc
     3301 gacaagtagt accgccattg acaccatagc tgaagtcgat gcaatttcag gcggaaattt
     3361 attgcaggat tatacggcca atgacctgac ccaaagtgtg acaggcttag cagctggcag
     3421 tacatatttc tttgcagttg tcgttcgaga ccaagccaac aacaaagcta tctatacgcc
     3481 gaggatgcga tactcttgga caaatcaaac cctacctagc tcatctcaat ggagatcagt
     3541 aacttttggg aatggcgtat tcgttgcagt cgcaagtggg cccagtacaa cagccgcgac
     3601 atcacccgat ggaaaaactt ggacggcacg aaccttacct agctcgcaaa actggaactc
     3661 cgtgaccttc gcgaatggaa tatttattgc acttgccggt ggcaccaatg cagccacctc
     3721 accggatgga ataacgtgga ctatgcgaac aatgtccagt tttgggagct ggcaatctgt
     3781 cgcttttggc aatggggtat attcagcgat agactacggc agcaccgctg cgtccacctc
     3841 accggatgga ataacgtgga cgcaacaaac cgtgccaacc tcagcgaact ggcaatctgt
     3901 tgcttttggc aacggtgctt tcactgcgat agcttatggc agcaatattg cggccacctc
     3961 accggatggc ataacgtgga cgatgcgaac cctgccgagc tcagtcaact ggaaggaagt
     4021 gacatttgca aatggcatat ttgttgcagt ggcttcaaat acatcaacgg cagctacgtc
     4081 gccagatggg atcaactgga cagctcgaac gatgagcaat ttttacacaa attctgtaac
     4141 gtacggtaat ggcgtttttg tttcggtctc ggctagcgct cagggcgggt caacgtcacc
     4201 agatggcgta acgtggactg atacagttgg tgccttctca ggtacttcgg ttgcctttgg
     4261 gaacggcgtc tttgtgtcag tagaaataaa tagtacaaat gcagcgacat tgccatagtt
     4321 aaaaactcac agcggcgagg gggaagactc tggacgtaac tttaaatgtc aaaaagaccc
     4381 gcatgccgag tagcagctgc gactatcata ccacagagca ggcaccacaa tttatgcggg
     4441 tggggatttc agcatgatcc ttgatcaggt ctgcgaccgc attggagcca tcagcagtgc
     4501 tggctggccg atttccagct ggttgccaaa cgccaatggc acagtgtaca caattgcgat
     4561 ttcaggtagc acggtctatg ctggtggaaa ctttacttcg atcggcgggg cacttcgcac
     4621 gcgaattgca gcgctggatt cttcaggtat tgccctcgct gcatttggaa cgagcgcaaa
     4681 tggcatagtg cgctccattg ttccagctgg cagtacactc tatgtcggag gagacttttc
     4741 cattattggc ggtggaaatc gcaatcggat tggagcgttg gattcaacca caggagcagg
     4801 taccagctgg gttccggagg ctaacggcag tgtttattcc ctagcaatcg tgggcacgac
     4861 tgtgtatgca gcgggcaact tcacaaccat tggcggccag tcacgcagtc gcatcgccgc
     4921 gcttgatatg acaatcaaca caaacaacgc aactagctgg aatcctagcg catcagggct
     4981 tgtatggagt ctctccgcct ctggtacaac agtcaacatg ggcggcgcgt ttagtaccgt
     5041 cggtggattc ggcatttcga atatagctgc catcgacacc acaaccagca gcgtactgga
     5101 atactagcca tgaaactcgc cgccttcttc accctcatcc tctccaccgc gaccctccac
     5161 gcccaagcac tcaagcgcgt gatgatcctc gatttcaaaa atctggataa aaacccggat
     5221 tacacctacc tcgaagagtc gatcaccgag gcggtacgca acgatctgaa agcgaagttt
     5281 gactttcgcg agatgcaaaa atcggactgg cagaagctcg cagaaaaaaa cctgcacctt
     5341 tggcccgaag ataactacac gcgcagcttc ggtctacaca tcggtgctgt cgcccggcag
     5401 gatattgtcg ttgggggtta ttaccaggca atcattgaga aaaaaccgcg aaaaggagtg
     5461 cgggcgcatg acttcgtgat tcgcgctcac gtgtttgtca tcgatatcgg ccagcgtaag
     5521 gtcgtgagcg agtttgacat gatcatgcct gccgatgctt cgctctttac ttcgatagaa
     5581 gtacttgccg cacgcgttgt ggcggagtct aaagcggtac tccccaacaa aggggaattg
     5641 agccaggaag tgatcgacgc tgacgaacta tcaccacacg agatcggcat tttcggtggt
     5701 atgtcagtac tctcggtgcc cgcagcattc aacggtacat tcacgacaga taagaagctc
     5761 cacaacaaag atgtgccgca aacactcggt gtctatggca gctttcgtta ccacgatttt
     5821 atctggccgc gt
//
LOCUS       DAOQJF010000208         5810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_7887_length_5810_cov_5.895743, whole genome shotgun sequence.
ACCESSION   DAOQJF010000208 DAOQJF010000000
VERSION     DAOQJF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7887_length_5810_cov_5.895743"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLF85_11145"
     CDS             <1..364
                     /locus_tag="PLF85_11145"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE20266.1"
                     /translation="QRAPFTVAQYHSVRQLVRRLERDLDQKRCFPENPITGKILDVSH
                     GGIGFIYTGQRSILSSAGVADKVVFDAHFSADNIATFSGKIMNMTSLENGRRYGIEFE
                     HVSEGGQKALAQMIEGET"
     gene            428..2992
                     /gene="leuS"
                     /locus_tag="PLF85_11150"
     CDS             428..2992
                     /gene="leuS"
                     /locus_tag="PLF85_11150"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNE20267.1"
                     /translation="MSYPFSEIEKKWQEYWENNHTFRTPTDFSKPKYYVLDMFPYPSG
                     AGLHVGHPEGYTATDIMARKRRMQGYNVLHPMGWDAFGLPAERYAMQTGIHPSITTRQ
                     NIDNFRRQLKALGFSYDWEREVDTTSPDYYKFTQWIFLKLFNSWYDPQLKKARPISEL
                     PIPEELKSETKKKEREEFTANHRLAYIANMPVNWCAELGTVLANEEVDEWVGKGYKVE
                     RKPMRQWMLRITAYAERLLNDLQHVDWPAGTLELQKNWIGQSTGALVHFGIEGSSEQL
                     TVFTTRPDTLYGVTFMVLAPEHPLVDKIAAESQRGAIEEYRKTTALKTDLDRQLDAAK
                     KEKTGVLTGAHAIHPLTKEKIPLYIADYVLMGYGTGAIMAVPGHDERDFAFAQQFGIA
                     VKQVVVPLPASPASGGGAESGGGELPYAADGIAVNSPIFDGLKTTEAKAKIIEHLEKE
                     KLGERRIQYKLRDWLFSRQRYWGEPVPVSFDEDGAMVAEDEKDLPLLLPESKSFQPAG
                     TGESPLANLTDWVIFEKNGRKLRRETNTMPQWAGSSWYYLRFADPANKERFASADAEK
                     YWLNGGVDLYVGGAEHAVLHLLYARFWHKVLYDLGYVTSIEPFKKLVHQGIILGEDGT
                     KMSKSRGNVINPDTVVAEYGADAFRLYEMFMGPLEQMKPWSSKSIEGIYRFLARVHRL
                     YLNEGILNEKLLAAPADAKAVLKVLHQTIKKVSDDIETLSLNTAISQMMIFVNEAYKF
                     ESIGKEAAEIFLKLLSPFAPHITEELWQALGNSGSIALAEWPQHNPEHLVESSFEAVI
                     QINGKIRARTQLAVGVSDTEFEQAARAIDTVQAALDGKSVKKVIVVKNKMINFVVA"
     gene            3028..3549
                     /locus_tag="PLF85_11155"
     CDS             3028..3549
                     /locus_tag="PLF85_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNE20268.1"
                     /translation="MSKSELRERFKIMAKEFFASQNAAALTTMHEQIAVALKNYAQRN
                     LPYELRKEPRVAVYRPMKVELPAREIVSASAVFERPVYLYPEIDGEKMWFTDENGALA
                     EPEIVIVPGLFVDKAGNRLGRGKGYYDRFLAESQVPVTQRIFLGYDFQFIASVPTDER
                     DEQVTPVHPVKAN"
     gene            complement(3550..3822)
                     /locus_tag="PLF85_11160"
     CDS             complement(3550..3822)
                     /locus_tag="PLF85_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20269.1"
                     /translation="MTFEATGALWAIFATIVTGFGIASLMVHLEYVRSRKMNDWREEF
                     RIQQMESNRRLQRIHDENTRFFRSMGLTTQQIFERVDRGLDWDRTE"
     gene            complement(3874..4278)
                     /locus_tag="PLF85_11165"
     CDS             complement(3874..4278)
                     /locus_tag="PLF85_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE20270.1"
                     /translation="MNTIAEELGGEATPTEVKNGKSFRTQWNYVQVSGRIASDVEKKT
                     VKEQTVISFTLLFETVRKTDKEGSHANFIQVELWGKMAELFYPLLAKGIEIMVTGELF
                     QQRWVGTDGKKAQKYSISAQALAISDQKFRPS"
     gene            4399..>5810
                     /locus_tag="PLF85_11170"
     CDS             4399..>5810
                     /locus_tag="PLF85_11170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE20271.1"
                     /translation="MATRRAKTPTKKKRIVIIGAALAGPTAAARAREIDETAEIVLLE
                     RNTRVSYAMTGLALHLSGEVKSLEDLNREREDFFREVYNVDVRTRTEVMSIDAKKKQL
                     LLRQNSGEEILAYDKLIFATGASSVQPIGIDEADNFRFFRTLDDLAAIRASLDAGRKR
                     FVVLGGGSMGAEALDGLVRGGADVTLLEKKAQFLPDYSPEIALTAMTALEHKVRILTA
                     VKDITFEKKNGVITAVKADGVRIETDFVVSAIGVRPRTELLKKAGVKLLPDGSIPVND
                     RCQTNIKDIYACSICVSIPEDKDYRWIPQAAVSDKTAQVAAENAAGGSARLGPVTQSQ
                     HIRLPGIEIGRVGLTCAQALRKYGKANIDSVFIHGRDKEAYMPGSAHLGLKLYYHKKK
                     REVIALEVVGEEIKSRLDAFATALAGKLKLHDLALIDLAYTPPFGTARDALNVAATVA
                     LQKLEKLTTTVTYEEIRAKR"
BASE COUNT         1427 a         1589 c         1574 g         1220 t
ORIGIN      
        1 ccagagagca ccgtttaccg tggcacagta ccactcggtg aggcagctgg tgcggcgact
       61 cgaacgcgac ctcgatcaaa aacgctgttt tccggaaaac cccatcaccg gaaaaatact
      121 cgatgtcagc cacggcggta ttggttttat ctacaccggc cagcgcagca tcctgtcgtc
      181 cgccggcgtt gccgacaagg ttgtctttga cgcacatttt tctgcagata atatcgctac
      241 ctttagcggc aaaatcatga acatgacatc gctggaaaac ggcaggcgct atggtattga
      301 attcgagcac gtctccgaag gtgggcagaa ggctcttgcc cagatgatcg aaggcgaaac
      361 ctgaccggtc agcaggccgt ggcactgata acgatttgcg ccgtgtcgcg cagttgaatt
      421 gcagcgtatg tcgtatccgt tttccgagat tgagaagaag tggcaggaat actgggaaaa
      481 taaccatacc ttcagaactc ccactgattt ttctaagccc aaatactatg tactggatat
      541 gttcccctac ccctcgggag caggtctgca cgtcggtcac cctgagggtt acacggcgac
      601 cgacatcatg gcgcgcaagc ggcgcatgca gggctacaat gtacttcacc caatgggctg
      661 ggatgccttc ggcctgccgg cagaacgcta cgcgatgcag acaggaattc acccgtcgat
      721 tacgacgcgg cagaatatcg ataacttccg caggcagctc aaggcgctgg ggttctcgta
      781 tgactgggag cgcgaagtcg acacgacttc acccgattac tacaagttca cgcaatggat
      841 tttcctgaag ctcttcaact catggtatga cccgcagctg aagaaggcaa gacccatcag
      901 tgaactgccg atacccgaag aactcaagag cgaaacaaag aagaaagagc gtgaagaatt
      961 cacagcgaac caccgccttg cctatatcgc caacatgccg gtgaactggt gtgctgaact
     1021 cggcaccgtg cttgccaatg aagaggtcga cgaatgggtc ggcaaaggct acaaggtgga
     1081 gcgcaaaccg atgcggcagt ggatgctccg tattacagcc tacgctgagc gcctgttgaa
     1141 tgatttgcag catgtcgact ggcctgccgg tacgctcgaa ttgcaaaaga actggattgg
     1201 ccagtcaacc ggtgcacttg tgcacttcgg cattgaaggc agcagcgagc agctgacagt
     1261 cttcactacg cgaccggaca ctttgtacgg cgttacattc atggtgctgg ctcctgaaca
     1321 tccgctcgtt gataagattg ctgcagaaag ccagcgcggg gcgattgaag aataccgcaa
     1381 gacgaccgca ctcaagaccg atctcgaccg ccagctcgat gcggcgaaaa aagaaaagac
     1441 cggtgttttg accggagcac atgcgatcca cccactgacg aaagagaaaa tccccctgta
     1501 tatcgccgac tatgtactga tgggctacgg cactggcgcg attatggctg tgcccgggca
     1561 cgacgaacgg gacttcgcgt tcgcccaaca gttcggcatt gcggttaagc aggtcgtcgt
     1621 ccccctccca gcgtcccccg caagcggggg aggtgctgaa agcggagggg gagaacttcc
     1681 ttatgctgct gacggcatcg ccgtgaactc accgattttc gacgggttga aaaccacaga
     1741 agcaaaagca aaaatcatcg agcacctcga aaaagaaaag cttggtgaac gccgcataca
     1801 gtataagctg cgcgactggc tgttttcgcg ccagcgctac tggggcgaac ctgtacctgt
     1861 cagcttcgat gaagatgggg caatggttgc cgaagacgaa aaagacctgc cgctgctctt
     1921 acccgaaagc aaaagttttc aaccagccgg cacgggcgaa tcaccgctcg ccaacctgac
     1981 tgattgggtt atttttgaga aaaatggccg caaactgcgc cgtgaaacca acacgatgcc
     2041 acaatgggcg ggcagctcgt ggtactacct gcgctttgca gacccggcga acaaagaaag
     2101 attcgcctcg gcagacgcag aaaaatactg gttgaacggc ggtgtcgacc tctacgtcgg
     2161 tggcgccgaa cacgccgtac tgcacctgct ctatgcgcgt ttctggcaca aggtacttta
     2221 tgacctgggt tacgtgacgt cgattgaacc gttcaaaaag ctggtacacc agggtatcat
     2281 tctcggcgaa gacggcacca agatgtcaaa atcgcgcggc aacgtgatca accctgacac
     2341 cgtagtggca gaatacggcg ccgatgcctt tcgcctgtac gaaatgttca tgggtccgct
     2401 cgaacagatg aagccgtggt cttcgaaaag tatcgagggc atctatcgct ttctggcgcg
     2461 cgtgcaccgg ctctacctga atgaaggcat actgaacgag aaactgcttg ctgccccggc
     2521 cgacgccaag gcagtcctga aagtgctgca ccagaccatc aagaaagtga gcgacgacat
     2581 cgagacactt tcgctcaaca cagcgatttc gcagatgatg atctttgtca acgaagcata
     2641 caaattcgag tccatcggca aagaggcggc ggagatcttt ctcaagctgc tctcaccctt
     2701 cgccccgcac atcacagaag agttatggca ggcgctgggt aacagcggca gtattgccct
     2761 ggcagaatgg ccgcagcata acccggagca cctggtcgag tcaagcttcg aagcggtcat
     2821 acagatcaac ggcaagattc gcgcgcgcac gcagctcgct gtcggcgtga gcgatacgga
     2881 gttcgaacaa gcggcgcggg cgatcgacac ggtgcaggca gcactcgacg gcaagagcgt
     2941 gaagaaagtt attgtcgtga agaacaagat gatcaatttc gtggtcgcct gacatgcagg
     3001 gctctgccct gtatgtcagg caaaatcatg tcaaaatctg agctccgcga acgcttcaag
     3061 atcatggcaa aggagttctt tgccagccaa aacgccgcgg cgcttaccac aatgcacgag
     3121 cagattgccg tagccctgaa aaactacgcg cagaggaatc tgccttacga gctacgcaaa
     3181 gaaccacgcg tggcagttta ccggcctatg aaggttgaat taccagcgcg ggaaattgtc
     3241 agcgcctcgg ctgtctttga gaggccagtt tatctttatc ctgagatcga tggcgagaaa
     3301 atgtggttca ccgatgaaaa cggtgcgctt gccgaacccg aaattgttat agtgccaggc
     3361 cttttcgtcg ataaagcggg taaccgatta ggcaggggca agggatacta cgaccgcttt
     3421 ttggctgagt cgcaggtacc cgtcacccag cgtattttcc tgggctatga tttccagttt
     3481 attgcgagcg tacccacaga tgagcgggat gagcaggtga cgcctgttca tcccgtaaag
     3541 gcgaactgac tattcggttc ggtcccaatc gaggccgcga tcgactcgct cgaagatctg
     3601 ctgggtagtc aggcccatgc tgcgaaagaa gcgcgtgttc tcgtcgtgaa ttctctgcag
     3661 gcgccggtta ctctccatct gctggattcg aaattcctcg cgccagtcat tcattttgcg
     3721 actacgaacg tattcgaggt ggaccatgag gcttgcaata ccgaagcctg tcacaatcgt
     3781 cgcgaagatc gcccacaaag cgcctgttgc ttcaaaagtc attttgcctt cttgtttgca
     3841 ggctataccg aaaactgcgc cgcgtcaaca tttttagctc gggcggaatt tctggtcgct
     3901 gattgccagc gcctgcgccg aaatcgagta cttctgcgcc ttcttgccgt cggtaccaac
     3961 ccaacgctgc tggaacagtt cacccgtgac cataatctcg atacctttgg caagcaacgg
     4021 gtagaagagt tcggccatct tgccccatag ctccacctgg ataaagttgg cgtgtgagcc
     4081 ttctttgtcg gtcttgcgca cggtttcaaa aagcagcgta aaactgatta ccgtctgttc
     4141 cttcactgtt ttcttttcaa cgtcggaagc aatgcgccct gaaacctgca cgtagttcca
     4201 ctgcgtgcga aagctcttgc cgttttttac ctctgtgggg gttgcttcgc cacccaattc
     4261 ttcagctatt gtattcattt tccttcctta gagggtttat tccccctcta catattaaat
     4321 accaggaaac cgcgtttttt tctcaccctt tcgtaacttt tttcctttac gcagcttgcg
     4381 gaaaatgcat gctacgttat ggccacccgc agagcgaaga ctcccacaaa aaagaagcgc
     4441 attgtgatta tcggcgcagc gctcgcaggc cctactgctg cagcgcgcgc gcgcgaaatt
     4501 gacgaaacgg cagagattgt gctgctcgag cgcaacacgc gcgtcagtta cgcgatgaca
     4561 ggccttgcac tgcatttaag cggcgaggta aaatcgctcg aagaccttaa ccgggaacgc
     4621 gaagattttt tccgcgaagt gtacaacgtc gacgtgcgca cccgcaccga agtcatgtcg
     4681 atcgacgcaa aaaagaaaca gctgctgctg cgacaaaata gtggcgaaga gattctggcc
     4741 tacgacaagc tcatcttcgc aacgggagct tcttcggtgc agccgattgg tatcgacgaa
     4801 gcagacaatt tccgtttctt caggacactc gacgacctcg ctgcgatcag ggcgagcctc
     4861 gatgcgggtc gcaaacgttt cgtggttctc ggcggtggtt caatgggagc cgaagcgctc
     4921 gacggcctgg tgcgcggtgg cgcagacgta acgctgctcg aaaagaaagc acagtttctt
     4981 ccagactatt cgccggaaat tgcccttacg gcaatgaccg cgctggagca caaggttcga
     5041 atacttaccg cggtcaaaga tatcactttt gagaagaaga atggcgttat cacggcggtc
     5101 aaagccgacg gcgtacgcat cgaaacagat tttgtcgtgt cagcaattgg tgtcaggccg
     5161 cggactgaac ttctgaagaa agccggcgtg aaacttttgc ccgatggctc gataccggtt
     5221 aacgaccgct gccagacgaa catcaaagac atctacgcct gcagcatctg cgtcagtata
     5281 cccgaggaca aagactaccg ctggataccg caggccgcgg tctctgacaa gactgcgcag
     5341 gtggcggcag aaaatgccgc cggcggcagt gcgcgactgg gtcctgtcac gcagagccag
     5401 catattcgcc tgccaggcat cgagattggc cgcgtggggt taacctgcgc gcaggctctg
     5461 cgaaaatacg gcaaggcgaa tatcgactca gtcttcattc atggccgcga taaagaagcg
     5521 tacatgcccg gatctgcgca cctgggtctc aagctctatt accataagaa gaaacgcgag
     5581 gtgattgcgc ttgaagtcgt gggcgaagag atcaagtcac gcctcgacgc ttttgccact
     5641 gcacttgcgg gtaaactgaa gctgcacgac ctggcgctca ttgacctcgc atacacaccg
     5701 ccgtttggca ccgcgcgcga tgccctcaac gtagccgcca ccgttgcgct gcagaaactc
     5761 gaaaaattga cgacgacagt tacttacgaa gagattcgcg cgaaaagaaa
//
LOCUS       DAOQJF010000209         5743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8032_length_5743_cov_4.791491, whole genome shotgun sequence.
ACCESSION   DAOQJF010000209 DAOQJF010000000
VERSION     DAOQJF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8032_length_5743_cov_4.791491"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLF85_11175"
     CDS             complement(<1..434)
                     /locus_tag="PLF85_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNE20272.1"
                     /translation="MTAAPELSKEIDETIARFAAEPDNLILILHAIQDAHGYVPRESA
                     LLLSSRLSVPLARIYEVLTFYHYFHTSPPGQHQVVVCHGTSCWLNGSSEVLAAACEYA
                     ANHPGTTVNEVRCVGCCGLSPLVFADKQIHTHLDAEKTTALL"
     gene            593..1978
                     /gene="tig"
                     /locus_tag="PLF85_11180"
     CDS             593..1978
                     /gene="tig"
                     /locus_tag="PLF85_11180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNE20273.1"
                     /translation="MPSISISRESDTRVKADITVNSEEIKPAEEQAIKKVSSKAEIKG
                     FRKGKAPAHLIKAKFAPDIRLETLFALVDNHLSEVEKKMEERVYRLVRIEDVQEKGDK
                     MSFAVKLDLFPYVKVGKLKVAQLTRHVPTIDDSDIEQEIKDRLLRFAEFVDASEGATA
                     DKNDKLTVDFEIWVDDAPSGEVNKDFQFDLGSGSLSRELEQQIIDKKGKVGEEFKLRK
                     DIPAQEDGNTRSYEIIATIKKISKPKLPELTDAFVAEKFRGSNTVAEFKNKVREELEK
                     RFDVALIRSETDKALKTLEESAQFFLSESFVDQKLEEFLEERKIERDGLTAPQVENLR
                     KALGDREKPGLLMRQLINDAEKNYQKKNKTDLTYIEAFRKYVHDEITRFEGVGERSEA
                     LLKELDAVIDAMISGETNRSPWESLISSYLTVFSRDFLFNYFDDEGIVKKGKKLTYKE
                     LAAQLAQNAPE"
     gene            1965..2840
                     /locus_tag="PLF85_11185"
     CDS             1965..2840
                     /locus_tag="PLF85_11185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE20274.1"
                     /translation="MRPNNGMVTYPTARLKLCIVASLLAVLPGLSADTGKQSSWQVMA
                     ERAQAMLEKTEVEIDNRLIQTYQGYAGVWISSGANVAVSKVCREKICDEPLFRVINEI
                     PTDHKYLRRSGLFFLPYTKEQMLAFKETGVERLKLQLKKGEYLWPVMGLRVTSRVGTR
                     WGKLHGGIDVAAARGSIVVGATEGTVMIVGDQGAYGHCVFVENHDGTVAWYAHLTESV
                     VKVGDKIVRGQIVGVSGNTGRSTGPHLHFEMRTQQGIILDPEHFFFLPYEEHLKQAMQ
                     HESELGGKAKPVGKF"
     gene            2842..3831
                     /gene="hemB"
                     /locus_tag="PLF85_11190"
     CDS             2842..3831
                     /gene="hemB"
                     /locus_tag="PLF85_11190"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNE20275.1"
                     /translation="MMNQPAYLRERPRRNRFSAGVRALTSETRIDVAKLIEPLFLIDG
                     AARSEEIRSMPGIFRFTEDLALRHVESSLKLGIRSFILFPAVEDALKDSVGSYSYNPK
                     NFYLRIIRSLKQHFPEAVLISDVALDPYSSDGHDGIVRNGRIVNDETLEVLGKMSLAQ
                     AEAGIDILGPSDMMDGRVGYLRDALDSGGFTDVGIMSYCAKYASAFYGPFRDALASAP
                     KFGDKKTYQMDPANALEALREARLDTAEGADFLMIKPALAYLDVIRRIKDASDLPVVC
                     YNVSGEYAMVKAAADKGWLDGARARDEMLLSFFRAGADAVITYFAHEYAAAHS"
     gene            3828..4745
                     /locus_tag="PLF85_11195"
     CDS             3828..4745
                     /locus_tag="PLF85_11195"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNE20276.1"
                     /translation="MSDYIKTVFWQIEITALKEHAAEIEDFFLRSGAQSTFELLYVEG
                     RTSNLVEDNTQLYFFFPEEFPARAFVPMALATLGFPDLPFEVGQVRYADYLKEFERTF
                     RAFRLTDRTALVPPWDTENPETSDVEKKLWLVPGMAFGTGKHATTQLMVEFIENTVRP
                     TDTVIDIGSGSGILAIAAVLWGAGSAWGVDVETLAVESATANKRLNEEQHKAAYACEF
                     AVGDFSALHQHTIDAAHTVFVANILPNIFEANAKDLQYGLTHCRAWALSGIPEAQQES
                     FTTFLRQIWPGTFEIRTKEDWQIYFFAGG"
     gene            complement(4746..>5743)
                     /locus_tag="PLF85_11200"
     CDS             complement(4746..>5743)
                     /locus_tag="PLF85_11200"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNE20277.1"
                     /translation="CWHNTGEGGFSPYHTSGADVVWQIGTGYFGARDENGFRLEKVVE
                     KVDQFPQIRMIELKLSQGAKPGKGGILPATKVTAEIAAIRGVKKGHDCISPNFHSEFS
                     TVDEMIRFIERIAAATGLPVGIKSAIGELDFWQELATRMKQTKKGPDFIVIDGGEGGT
                     GAAPLTFADHVALPFKVGFARVYQIFQKAKLSERVTFIGSGKLGFPDRAVVALAMGCD
                     LVNVAREAMMSIGCIQAQKCHDGHCPAGVATQNWWLQRGLDVDDKKNRAARYIKGFRK
                     ELLSLSHAAGYEHPAQFTGEDIEISVGLNRYKTLTEVLGYRRDVVKFPGMAKLVA"
BASE COUNT         1332 a         1637 c         1580 g         1194 t
ORIGIN      
        1 tgcagtagtg cggtagtctt ttcggcatcg aggtgggtgt gtatctgctt gtcggcaaag
       61 accagtggtg aaagtccgca gcaaccgacg cagcgcactt cgttcacggt tgtaccgggg
      121 tggtttgctg catattcgca ggccgctgcg agcacttcac tgctgccatt cagccagcaa
      181 ctggtgccgt ggcagacgac gacctgatgc tgccctggcg gcgaagtgtg aaaatagtgg
      241 taaaacgtta gaacctcgta gatgcgcgcg agcggtaccg aaaggcgcga cgataagagc
      301 agcgccgatt cgcgcggcac gtaaccgtgc gcgtcctgta tggcatgtag aatcagtatc
      361 aggttgtcgg gctcggcagc gaaacgcgcg atcgtttcgt cgatttcttt gctgagctcg
      421 ggtgcggcgg tcattgccta cagcgaatac aagacccgta tatccagtaa agcacttgtc
      481 ggcctgcctg ccacaccggt ggatatcggc tgctcgcatc ctgcggcgca gcccatccgg
      541 ctcaatttcc cccttaattg acgcgctgtt tcagaatcac aacttgccgg gtatgccatc
      601 tatatccatc agccgcgagt cagatacccg cgtgaaggct gatatcaccg taaattccga
      661 agaaattaag cccgccgaag agcaggccat caagaaagtc agctccaagg ctgaaatcaa
      721 gggctttcgc aagggcaaag ctcccgcgca tctgatcaag gcgaagtttg cgcccgacat
      781 ccgcctggag acgctgtttg cgctcgtcga caaccacctc tccgaagtcg aaaagaaaat
      841 ggaagagcgt gtctaccgcc tcgtgcgcat tgaagacgtg caggaaaaag gcgacaagat
      901 gtcgtttgcc gttaagctcg atctctttcc gtatgtcaag gtcggcaagc tgaaagtcgc
      961 gcagctgaca cgccatgttc cgacgattga cgactctgat attgagcagg agatcaaaga
     1021 ccgcctgctg cgtttcgccg agttcgtcga tgccagcgaa ggcgcaacag ccgacaagaa
     1081 tgacaagctg accgttgatt ttgaaatctg ggtcgacgac gcgccgagcg gtgaagtcaa
     1141 taaggacttc cagttcgacc tggggtctgg ttcgctcagc cgtgaactcg agcagcagat
     1201 catcgacaag aagggtaaag tcggtgagga attcaaactg cgcaaagaca ttcccgcgca
     1261 ggaagacggc aatacccgtt catacgaaat tattgccacg atcaagaaaa tctccaaacc
     1321 aaaactgccc gagctgacgg acgcgtttgt ggcagagaaa ttccgtggct ccaataccgt
     1381 agccgaattc aaaaataagg tgcgcgaaga actggagaaa cgtttcgacg ttgccctgat
     1441 tcgttctgag accgacaagg cgctcaagac actggaagaa agtgcgcagt tctttctctc
     1501 agaaagtttc gtcgaccaga agctcgaaga attcctggaa gagcgcaaga tcgaacgcga
     1561 tggcctgaca gccccgcagg tcgagaacct gcgcaaagca ctcggcgacc gcgaaaaacc
     1621 cggcctgctg atgcgccagc tgatcaacga tgccgagaaa aactaccaga agaaaaacaa
     1681 gaccgacctc acctacatcg aagcattccg caaatatgtc cacgacgaaa tcacgcgctt
     1741 tgaaggtgtg ggcgaacgtt ctgaggccct gctgaaagaa ctcgatgccg tgatcgacgc
     1801 catgatctcc ggcgaaacga accggtcacc gtgggaatct ctgatttctt catacctgac
     1861 ggttttcagc cgcgatttcc tgttcaacta cttcgacgac gaaggcatcg tgaaaaaagg
     1921 caaaaagttg acctacaagg agcttgctgc gcaattggcg cagaatgcgc ccgaataacg
     1981 gcatggtcac ctaccctaca gcccgcctaa aactctgcat cgtcgccagc ctgctggcgg
     2041 tgttgcctgg cctttcggcc gataccggca agcagagcag ttggcaggta atggccgagc
     2101 gggcgcaggc gatgctcgag aagaccgaag tcgaaatcga caaccgcctg atacagacgt
     2161 accagggtta tgccggcgtc tggatcagca gcggcgccaa tgtagcggtg agcaaagtct
     2221 gccgtgagaa gatctgcgat gaaccgctgt tccgcgtcat caatgaaatt cccacggacc
     2281 acaaatacct gcgccgctcg gggttgtttt ttctgcctta tacaaaagag caaatgctgg
     2341 cattcaaaga gacaggcgtt gaacgcctga agctccagct gaaaaaaggc gagtacctct
     2401 ggcccgttat gggcctgcgc gtcaccagcc gcgttggtac gcgctggggc aagctgcacg
     2461 gcggcatcga cgtggcggca gcgcgcggca gtatcgtcgt gggtgcaacc gaaggcacgg
     2521 taatgatcgt cggtgaccag ggtgcgtatg gccattgcgt gtttgttgag aaccacgatg
     2581 gtacagtggc ctggtatgca cacctgactg aatcggtggt caaggtcggc gacaagattg
     2641 tccgcgggca gatcgtcggc gtttcaggaa ataccggccg ttccacaggt ccacacctgc
     2701 actttgaaat gcgcacacag cagggtatca ttctcgaccc cgaacacttt ttctttctgc
     2761 cttacgaaga acacctgaaa caggcgatgc agcacgaaag tgaacttggc ggcaaggcaa
     2821 agcctgtcgg taaattctga catgatgaac caacccgcgt acctgagaga acgcccgcgc
     2881 cgcaacaggt tttccgctgg tgttagggcg ctcaccagcg aaacccggat cgatgtcgca
     2941 aagctgatcg agccgctgtt cctgatcgat ggcgccgcgc gcagcgaaga gatccgctcg
     3001 atgcctggta tcttccgttt caccgaagac ctcgcgctca ggcacgttga atcgtcgcta
     3061 aaactcggca tccgcagctt catcctgttt ccggcggtcg aagatgcgct gaaagactct
     3121 gtgggatctt actcgtacaa cccaaagaat ttctacctgc gtatcatccg tagcctcaag
     3181 caacattttc ccgaggccgt actcatctct gacgtggcac tcgaccccta ctcgtctgac
     3241 ggtcacgacg gtattgtacg caacggccgc atcgtgaacg acgaaactct cgaagtattg
     3301 ggcaagatgt cgctggcgca ggccgaagcc ggtatcgaca tactcggccc ttcagacatg
     3361 atggatggtc gcgtcggcta cctgcgcgac gcgctcgaca gcggcggctt taccgatgtc
     3421 ggtatcatgt cttattgcgc caaatacgcg agcgccttct atggtccgtt tcgcgatgcg
     3481 ctggcgtctg cccccaagtt cggcgacaag aaaacatacc agatggaccc ggcgaatgcg
     3541 cttgaagcgc tgcgcgaggc taggctcgac acggccgagg gtgcggattt cctgatgatt
     3601 aaacctgcgc tcgcctacct cgatgtcatc cgtcgcatca aagatgcttc cgatctgccg
     3661 gtcgtctgct acaacgtgag cggcgagtac gcgatggtca aagccgcggc tgacaagggc
     3721 tggctcgacg gcgcgcgggc acgcgacgag atgctgctgt cgttctttcg cgcgggcgcc
     3781 gatgctgtga tcacctactt cgctcacgaa tatgcggccg cacattcatg agcgactaca
     3841 taaagaccgt cttctggcaa attgaaatca ccgcgctcaa agaacacgcg gcagagattg
     3901 aagatttttt cctgcggtca ggcgcacaga gtacattcga gctcctctat gtcgagggca
     3961 ggacttcaaa cctcgttgaa gacaacaccc agctgtattt ctttttccct gaagaattcc
     4021 cggcacgcgc tttcgtaccg atggcgctcg ccactctcgg atttcctgac ctgcccttcg
     4081 aagttggcca agttcgctac gccgactacc tgaaagaatt tgaacgcacc tttcgcgcct
     4141 ttcgcctgac cgacagaact gcgcttgttc cgccgtggga taccgaaaac ccggaaacca
     4201 gcgatgtgga aaaaaaactc tggctggtgc cgggtatggc tttcggtacg ggaaagcatg
     4261 caacgacgca gctcatggtc gagttcatcg agaacacggt acgccccacc gacacggtca
     4321 ttgatatagg cagcggctcg ggtattctgg cgatcgccgc agtgctctgg ggagcgggct
     4381 ctgcctgggg tgtagacgtc gagacattgg cagttgaatc agccacggcc aacaagcggc
     4441 tgaacgaaga gcagcacaag gctgcttatg cctgcgaatt tgcggtagga gatttttccg
     4501 cactccacca gcatacgatc gacgccgctc acaccgtttt cgtggccaat attctgccaa
     4561 atatcttcga agcaaatgcc aaagatttgc agtatggcct tacccactgc agggcctggg
     4621 cgctctcggg aattcccgaa gcccagcagg aatcgtttac gaccttcctg cggcagatct
     4681 ggcccggaac atttgaaatc cgcaccaaag aggactggca gatttacttt tttgccggtg
     4741 gctgatcagg ccaccagctt tgccataccc ggaaacttca ccacatcacg gcggtagccg
     4801 agcacttctg tcagagtctt gtagcggttt aagcctacgc tgatctcgat atcttcgccg
     4861 gtgaattgcg ctgggtgttc ataacccgcc gcatgcgaga ggctcaggag ctctttgcgg
     4921 aagcccttga tgtagcgagc cgcgcggttc ttcttgtcgt cgacatcgag cccacgctgc
     4981 agccaccaat tctgtgtcgc aacccctgca gggcaatggc cgtcgtggca tttctgtgcc
     5041 tggatgcagc cgatggacat cattgcttcg cgcgcgacat tcacaagatc acaccccatc
     5101 gccagtgcga caacagcccg gtctggaaac cccagcttac cagaaccgat gaaagttacg
     5161 cgctcggaga gttttgcctt ctggaagatc tggtacacgc gcgcaaaacc caccttgaac
     5221 ggcagcgcca cgtggtcggc aaaagtgagc ggtgcagcac cggtaccacc ctcaccgcca
     5281 tcgatcacga taaaatccgg acccttcttg gtctgtttca tgcgcgtcgc cagttcttgc
     5341 cagaagtcga gttcgccaat tgcactcttg atgccgacag gaagcccggt agcggcagca
     5401 atgcgctcaa taaaccggat catctcgtcg acggtcgaaa actctgaatg aaaattgggt
     5461 gagatacagt cgtgcccttt cttgacgccg cgaatggccg caatctcggc cgtcactttg
     5521 gttgccggca gaattccccc tttccctggt tttgcgccct gcgagagttt gagctcgatc
     5581 atcctgatct ggggaaactg gtctactttc tcgacgactt tttcgaggcg aaagccgttt
     5641 tcatcgcgcg cgccaaaata cccggtaccg atctgccata ccacgtcggc accactggtg
     5701 tggtaagggg aaaatccccc ctcgcctgta ttgtgccagc aat
//
LOCUS       DAOQJF010000210         5722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8072_length_5722_cov_4.119464, whole genome shotgun sequence.
ACCESSION   DAOQJF010000210 DAOQJF010000000
VERSION     DAOQJF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8072_length_5722_cov_4.119464"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..836)
                     /locus_tag="PLF85_11205"
     CDS             complement(162..836)
                     /locus_tag="PLF85_11205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE20278.1"
                     /translation="MQVRDAESARQRAGFDRLARFYDALLFLTSGNALHASQVDCLAR
                     IAAKSRILLVGGGTGRLAAKIARELAPERLLCVDLSTKMCESTERHLRKVPGCPSEVV
                     QADIRNFMPEGRFDVVMTPYLLDVFSAAELPDVVRRLEGMLERHGVWLVADFTHPDCF
                     NGPRRMLQKAVLALLYWFFRLTCSISARQLPDIDSVLFAHGFRKTWTSLRFFRTLSSK
                     AYSRQA"
     gene            914..1615
                     /locus_tag="PLF85_11210"
     CDS             914..1615
                     /locus_tag="PLF85_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HNE20279.1"
                     /translation="MQSGRVNREFLLRIRRNWGEFWQNNRRRNELLVSLALLTAVMLV
                     TLKFLHYNEQRSGVRVADPLLALIPPVDLSVLTFILLYMGLIATVSLLMAQPHRLLIG
                     IQVYAVYAALRMLSMWLIPLEPPDGMIPLRDPLMFWAHTGVQLNKDLFFSGHTATMFI
                     FYLVIPRSRTKSLFLVGFVIMACCLIVQRVHYSVDVLSAPFYCYGALGLVLRLRKSLR
                     LDADLHNWLSVSDHR"
     gene            1748..2641
                     /locus_tag="PLF85_11215"
     CDS             1748..2641
                     /locus_tag="PLF85_11215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE20280.1"
                     /translation="MANAEQKIKVRRINFGHETEEQVHWFRNNQVMTHFMNSLHSVFP
                     DGERLFIRSVKYFEKEVKYPELKERVKAFIGQEMQHGIQHERFLQALDKMGLEGTKFE
                     EWYSKNAYVSKEDKMSAERLLLTPLRWLFGDQTAERMSLSVTAALEHYTSTLGEVVLK
                     NEAYYTEGMPDSLKQLFLWHAAEEVEHKSVAYDVFHDVAGGNYLERQVGFAFGSFFLA
                     YYIGIGWAWYLAKDKERDLAKLPQDLLDSVPVYFNLLKDVIIGGVQYLKPGFHPDQMD
                     NDHLARELFSRYEAEFAQKSA"
     gene            2693..3331
                     /locus_tag="PLF85_11220"
     CDS             2693..3331
                     /locus_tag="PLF85_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE20281.1"
                     /translation="MNNHCCRVVLTGGPGGGKTTAADLYRREIGEGVVIVPEAATLLY
                     MGGFPRQGEGEVQKATQRAIYHVQKNLEDAHFANYQNRVLLCDRGTIDGAAYWPDKAD
                     DFFTHLDTTLEAELARYDAVIFFETAAVGGISIIEGGNPTRTESLELARTLDARLRNL
                     WSKHPNFFLVPHNASFIRKVNAGLAELEKFVTRYHNGAGGTSGSPAAPIPFG"
     gene            3362..3685
                     /gene="xseB"
                     /locus_tag="PLF85_11225"
     CDS             3362..3685
                     /gene="xseB"
                     /locus_tag="PLF85_11225"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNE20282.1"
                     /translation="MASKKAPTNDPAPPAATVKLPAGLTFEKALAELEAITEKLEDGK
                     ESLETSMQLYEQGLALKEFCDRKLKEAEGKWLILKKQKDGSVVEEEIPSDRIPEPNEL
                     QGQMF"
     gene            3682..4662
                     /locus_tag="PLF85_11230"
     CDS             3682..4662
                     /locus_tag="PLF85_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNE20283.1"
                     /translation="MKQEIRTAIEDTFRAAESSIAQNLAEPRFLNEVAMAAAMLSESS
                     NRILTCGMGKSGLIARKVAATLTSTGSPAVFLHPADALHGDIGNLQSKEVVLAFSNSG
                     ETREIVELLPHVKRLQGKLIAVTQKAESTLARDADAAVIYSITREGCPLELAPMASTT
                     ISLIIGDAISTALIKLKKFRREDFGKYHPSGTLGRKLLTRVRDVMQPAGEILLTRSSP
                     FKTFLPKMITANLGCMLIVGSKGHLRGILTDGDIKRQLEHFAAQPERLFSATAGDMMT
                     ENPFFIRDAALVDEAFALMQEKKTYTLPVVNAQKKPIGIVRMHDLMGTGG"
     gene            4671..5489
                     /locus_tag="PLF85_11235"
     CDS             4671..5489
                     /locus_tag="PLF85_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20284.1"
                     /translation="MAAKQTSIEEKVTRAVLKLQRDRIVVFRINALTRNGREELMTIF
                     TSIFTEFGLNPGLMFNLYSAVVEIIFNAIKANAKHVIFKSEVRQKLVKQNFADIDHLL
                     DIIFRQEVLRDFMTRHVVSDKLRKRVNTVLQIAEKKRTGKLDKLTRADLQHLADFNQL
                     LKKESAYIHFTMALTDESMVINVVNDIPILAKDMARIEQSRTTHKRLYDEGRSTDYFG
                     PEYLDQTESAGFGIAMADEIYYEMGLNPLDYFSIGASEGSTRAVMRFPRARLAL"
     gene            5495..>5722
                     /locus_tag="PLF85_11240"
     CDS             5495..>5722
                     /locus_tag="PLF85_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20285.1"
                     /translation="MPSVLSSVARRALVLLVAPLLVNILLFVAYVLAGPLFIDPATLA
                     TMRQTRPGNLLVIRLLAFAIPVAYVLIRSRGF"
BASE COUNT         1345 a         1630 c         1536 g         1211 t
ORIGIN      
        1 cggcgcatat ccaccgcgcc catgagagag cgcgaggctt cgactatttc gccgagcttt
       61 tcgaggcggc gcgatagtcg cagcgcttct tcgttactcg ggtacacacc ctctgcaagg
      121 cgcgccagta tttcgtgtgt atcgcgcgcc atcaggtatt cttatgcctg ccgtgagtag
      181 gctttcgaag agagagtgcg aaagaaacgc aaactcgtcc acgtttttct gaagccatgc
      241 gcaaatagga ctgagtcgat atccggtagt tggcgggcac tgatgctgca cgtcagccta
      301 aagaaccagt atagcagagc cagcaccgct ttctgcaaca ttcgccgtgg gccgttaaag
      361 caatcaggat gtgtaaaatc tgccacgagc catacgccgt gccgttccag cattccttcc
      421 agccggcgga ccacgtctgg cagttcagcc gcagagaaaa cgtcgagcag atacggcgtc
      481 ataaccacat cgaaacggcc ctcgggcata aaattccgta tgtctgcctg aaccacttcg
      541 gaaggacagc caggtacctt tcgcaggtgg cgctcggtcg actcacacat cttcgttgaa
      601 agatcaacgc agagcaaccg ctccggcgca agctcgcgcg caatcttcgc ggcaagtcga
      661 ccagtaccgc caccaaccag cagtatgcgc gattttgcag ctatgcgggc gaggcagtca
      721 acctggcttg catgcagcgc attgcccgaa gtcagaaaaa gcaacgcgtc gtaaaagcgc
      781 gccagccggt caaacccggc tcgctggcgg gcagattcag catcccggac ttgcacctgt
      841 ccccctcgca caggcatgca ccgcgtaaac cgattcgccg gactatgaga ttgacagaac
      901 acagcctgtg ccgatgcaat ccggcagagt gaaccgggaa tttctcttgc gcatacgccg
      961 taactggggc gagttttggc agaacaaccg gcggcgaaat gagttgcttg tatcgctggc
     1021 gttgctgacg gctgtgatgc tggtgacgct gaaattcctg cactacaacg agcaaaggtc
     1081 tggtgtgcgc gtggctgatc cgctgctggc tctcatacct cccgtcgacc tgagcgtgct
     1141 gacatttatt ctcttatata tgggcctgat tgcgaccgtg tcgctgttga tggcgcagcc
     1201 gcaccggctg ctgatcggca tccaggtcta tgcggtctat gcggcgctgc gcatgctctc
     1261 catgtggttg attccgctcg aaccccctga cggcatgata ccgctcaggg acccgctcat
     1321 gttctgggcg cacacgggag tacagctcaa taaagatctc tttttttcgg ggcatacggc
     1381 cacgatgttt atcttctacc tggtcattcc caggagtcgc accaaatcgc tgtttttggt
     1441 tggctttgtc atcatggcct gctgcctgat tgtacagagg gtgcactatt cagtcgatgt
     1501 actttcagcc ccattttatt gctacggcgc tttaggcctt gtattgaggc tgcgcaaatc
     1561 gcttcgctta gatgccgatc tgcataactg gctgagtgtc agcgaccaca ggtaaccggc
     1621 accgggcagg gattctgtgc acaagtgtac agtatttttc aaataaaaag ttgacgccgc
     1681 gttcaacttt gacgataatc taactgatgg gggcagcaaa aagcgctgca ggcccacgga
     1741 ggacagaatg gcaaacgcag aacaaaaaat caaggtgcgt cgcattaact tcgggcacga
     1801 aactgaagaa caggtgcact ggttcagaaa taaccaggtg atgacgcatt ttatgaactc
     1861 gctccactcc gtttttcctg acggagaaag gctatttatc cgcagcgtga aatacttcga
     1921 gaaagaagta aaataccccg aactcaaaga gcgcgtgaag gcctttatcg gccaggagat
     1981 gcaacatggc atccagcacg agcgctttct gcaggcactc gacaaaatgg gtctcgaagg
     2041 cacaaaattt gaggaatggt acagtaagaa cgcttacgtc tctaaagaag acaagatgtc
     2101 ggcggagcgt ctgctgctga cgccgctgcg ctggctgttc ggcgaccaga ctgcggagcg
     2161 catgtcgctt tcggtgaccg ccgcgctgga acactacacc tcaaccctgg gcgaagtggt
     2221 gctcaaaaat gaggcatatt acaccgaagg catgccagac agcctcaagc agcttttcct
     2281 ctggcatgca gctgaagaag tcgaacacaa gtcggttgcc tatgacgtct ttcacgatgt
     2341 ggcaggcggt aactacttag agcggcaggt aggtttcgct ttcggttcat tctttctcgc
     2401 ctactatatc ggcatcggct gggcatggta cctggcgaaa gacaaagagc gcgatctcgc
     2461 gaagctgcca caggacctgc tcgactcggt acctgtgtac ttcaacctgc tgaaagacgt
     2521 tatcatcggc ggagtgcagt acctgaaacc tggcttccac cccgaccaga tggacaacga
     2581 ccacctggcc cgtgaacttt tcagccgcta cgaggctgag ttcgcgcaga agtcggcata
     2641 agcgaaagtc ccgtttggtt ttcgcgctgt gtgcggaaac caaacgggtt acatgaataa
     2701 ccattgctgc cgggtcgtac tgaccggcgg gcccggcggt ggtaagacca cggctgctga
     2761 cctctaccgg cgggaaatcg gcgagggtgt tgttattgtt cccgaagcgg caaccctgct
     2821 ctacatgggg ggctttcccc gccagggcga aggcgaagtg caaaaagcca cccagcgcgc
     2881 catttaccac gtacaaaaaa atctcgaaga tgcgcacttt gccaactacc agaaccgggt
     2941 tctactctgc gaccggggta ctatcgatgg tgcggcgtac tggcccgaca aggctgacga
     3001 tttttttacc catcttgaca ccacgcttga ggcggagctc gcacgttatg atgccgtgat
     3061 cttttttgag acagccgccg taggcggcat ttcgatcatt gaagggggaa atcccacccg
     3121 gacagaatcg cttgagctgg cgcgcacctt agacgcacgg ctacgtaacc tatggtccaa
     3181 gcacccgaac ttcttcctcg tcccgcataa tgcatccttt atccgcaagg tgaacgcagg
     3241 cctcgccgag ctggagaaat ttgtcacgcg ttaccataac ggcgcgggcg ggacttccgg
     3301 ttcacccgcg gcgccaatcc cgtttggttg acgcggcata ttctaaagag gaataactcc
     3361 tatggccagc aagaaagctc ccaccaatga cccggctccc cccgcggcga cggtgaaatt
     3421 gccggcaggc ctcaccttcg aaaaggccct ggcggagctc gaagcgatca ctgaaaagct
     3481 cgaagacggc aaagaatcgc tcgaaacatc gatgcagctc tatgaacaag gcctggcgct
     3541 caaggaattc tgcgaccgca agctgaaaga ggccgagggc aaatggctga ttcttaaaaa
     3601 gcaaaaagac ggcagcgtgg tcgaagaaga aatcccgagc gaccgtatac cagaaccgaa
     3661 cgaactgcag gggcagatgt tttgaagcag gaaatccgca cggccattga agataccttt
     3721 cgggcagccg aaagttcaat tgcacagaat ctcgccgaac cgcgtttttt gaacgaagtc
     3781 gcgatggccg cggctatgct ctccgaatcg agcaaccgta ttctcacctg cggcatgggc
     3841 aaatcgggcc tcatcgcccg caaggtggcc gcgacactca cctcaacagg gtcgcccgca
     3901 gttttcctgc acccggccga tgccctgcat ggtgacattg gcaacctgca gtccaaagaa
     3961 gtagtactcg ctttctccaa ctcgggcgaa acccgtgaaa tcgtcgagct gctgccacac
     4021 gtgaaacggt tacagggaaa actgatcgcc gtgacgcaga aggccgagag cacgctcgcg
     4081 cgcgatgccg atgcagcagt aatctattcg atcacgcgcg aaggctgccc gctcgaactt
     4141 gcgccgatgg cttccacaac catttcgctg atcatcggcg atgccatctc taccgcactt
     4201 atcaaactga agaaattccg ccgcgaagac ttcggcaaat accatccatc gggcacgctc
     4261 ggtcgcaagc tactgacgcg ggtgcgcgac gtgatgcagc ccgccgggga aatcctactt
     4321 acccgttcat cgccattcaa gaccttcttg cccaagatga ttaccgcaaa cctcggctgc
     4381 atgctgatag tcggcagcaa gggtcacctg cgcggcattc tgaccgacgg cgacattaaa
     4441 cggcagctcg aacactttgc tgcacaaccc gaacggctgt tttctgccac tgcaggcgac
     4501 atgatgaccg agaatccgtt ctttatccgc gatgcagctc tggtagacga agctttcgcc
     4561 ctgatgcagg agaaaaaaac ctatacactc cctgtagtga atgcacagaa aaagccgatt
     4621 ggcatagtgc gcatgcacga cctgatgggc accggcggtt gaggcgcacg atggcagcga
     4681 agcagacatc aatcgaagaa aaagttacca gggcagtgct gaaactgcag cgcgaccgca
     4741 tcgtggtctt tcggatcaat gcactgaccc gcaatggccg agaagaactg atgacgattt
     4801 tcaccagtat ttttaccgaa tttgggctga accccgggtt gatgttcaat ctctactctg
     4861 ccgtagtaga aatcattttt aatgccatca aggcaaacgc gaaacacgtg atcttcaagt
     4921 ctgaagtgcg gcagaaactc gttaagcaga attttgccga cattgaccac ctgctcgaca
     4981 ttatctttcg ccaggaagtg ctgcgcgatt ttatgacccg gcacgtggtt tccgataagc
     5041 tccggaagcg cgtgaacacc gtgttgcaga ttgccgagaa gaaaagaacc ggcaagctcg
     5101 acaaactcac ccgggcagac ctacagcacc tcgccgactt taaccagctg ctcaagaagg
     5161 aatcagcata catccacttc accatggccc tgaccgacga gagcatggtc atcaacgtgg
     5221 tgaatgacat ccctattctc gcgaaagaca tggctcgcat tgagcagagc cgaaccacgc
     5281 ataaacggct ctatgacgaa ggccgctcca ccgactattt tggtcctgaa tacctcgacc
     5341 agacggaatc tgctggcttc ggtatcgcca tggctgacga gatttattac gaaatgggtc
     5401 ttaacccgct ggattatttt agcatcggtg cgtctgaggg ctcaacacgc gccgtgatgc
     5461 gtttcccccg ggcccggctg gccctgtgac ctcggtgcca tctgttttgt cctcggtagc
     5521 gcgcagggca ctggtattac tcgttgcccc cttgctggtt aacatcctgt tgttcgttgc
     5581 ttatgtcctg gccggtccgc tgttcattga ccccgcgaca ttagccacca tgcggcaaac
     5641 ccggcctggc aatctgctgg ttatccgcct gctggcgttc gcgatacccg tggcctatgt
     5701 gcttatccgc agccgtggct tc
//
LOCUS       DAOQJF010000211         5721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8076_length_5721_cov_5.345394, whole genome shotgun sequence.
ACCESSION   DAOQJF010000211 DAOQJF010000000
VERSION     DAOQJF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8076_length_5721_cov_5.345394"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1358)
                     /locus_tag="PLF85_11245"
     CDS             complement(228..1358)
                     /locus_tag="PLF85_11245"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNE20286.1"
                     /translation="MTSLKVDIEDLHITIAEETLNRVRKFWREHDFTLGIEMLDAQHM
                     WLIALVFHLESLIKDPQKRDDKQVEGVFQEALRYARTHFAAEEMVLAEYRFPDVDNHA
                     AQHHTFQENIRNLLLSPDNETGENAGKLNRFLQNWLIQHIKKEDMSYRDFFRQNNIDA
                     NTYFQRLVAGGSEYALNQNQLDLYAAISQHNEIIPGISNEVLHEIQRMWRSFSIRLYV
                     PLIDMQHLWLIKMVVELSEALKVDYDKRASLLTDLLPDVKQYILEHFAAEEAMMDALD
                     YPVRAGHKNQHALFVRTVTSHDADYATRTRQGASVLVHDLKEWLLSHIVIEDAKFAKL
                     CREKNAEALAISKKLIQDKKVQFKKAQILLYQYIVEQTKRSA"
     gene            1506..2141
                     /locus_tag="PLF85_11250"
     CDS             1506..2141
                     /locus_tag="PLF85_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20287.1"
                     /translation="MLKKLHHKVLAPAGGIALLFFTFLPVSAQDLPADPAADQVEKDL
                     RTNYARKYRGSNITELSSAGEGVLLEKKNAKGKTVEIKRKFPFNVSATKGKKQFQYEI
                     GVNYKLVKGKWVFSELGVGAAKEIAAAGQLAPAKAEVKKMFAEAVSAKFFNNQPVDGV
                     LLTDPDFAGGAYRYNAEFKVGGSQTCTDWDFILRQDGGSWKVADIMRGRCN"
     gene            2189..2857
                     /locus_tag="PLF85_11255"
     CDS             2189..2857
                     /locus_tag="PLF85_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNE20288.1"
                     /translation="MTQADLKFLKALAKNNDREWFNSHKSEFETARDNFLHLVSAFIF
                     GMGDIDESIAGVEPASCLFRIYRDVRFSKNKAPYKTHLGAFISSAGRKGFNTPGYYLH
                     IEPGGESVFGSGLYMPDKDRLAAVRRELSAPNSRVAAVFADKKLRAAFPQVLEDDKVT
                     RVPRGFDPQSPRAELLKLRHFSVFARLPDTQVTNRKLVQQLLSKAPLLKHFNDTLTQA
                     TRTR"
     gene            2928..3416
                     /locus_tag="PLF85_11260"
     CDS             2928..3416
                     /locus_tag="PLF85_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20289.1"
                     /translation="MPTHALKRTLFALLLVSLAPALFAWNKADDIPVENLPAEVKSVL
                     EKYVQILKESPSVDEAAAAFAQIAGGSLVNDNGSVSNNTRQFGLKKDFNNLKHYAWPI
                     RITRVNKTVSRGEGYGARKIAGDRYTIWIAKDDPAKGLPAPVTIIAPTGGEPKVVNVG
                     SY"
     gene            3466..4308
                     /locus_tag="PLF85_11265"
     CDS             3466..4308
                     /locus_tag="PLF85_11265"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNE20290.1"
                     /translation="MLRRYRLFILSLLLLPLVSCALLQGRFSEFTPDIRLKEVNLRGF
                     DFEGADLEYVYTIKNKIGFGITFSRLAFQIAVDGKRMVDVKNDKNVVIKANDSTEFTI
                     VQRVRYVETVEAIFEFAKKDSVAIALTGAVGVYINELIGTIEVPIEASKTVPVPKLPQ
                     VHFGSLDFERMNLSNPLNPQATFTLRFNVRNPNPFEVKIPKVDYAFTAAGTNVISGAR
                     ANQTLKPQADSLITVPVNLSGRNIVELVPKLRDLNSTDYRFTSAVEFAIMGQSVSLPF
                     YYPK"
     gene            complement(4309..5331)
                     /locus_tag="PLF85_11270"
     CDS             complement(4309..5331)
                     /locus_tag="PLF85_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HNE20291.1"
                     /translation="MRRLFLLLALSPVSATVIVQKNGDVITGTILQEKNDRYIFRSPY
                     GQLRIKKSDVSRLILDEKTLELKNISHDNKVVQARLVSEDENTKVYLTDDGKTIRQQQ
                     VQSTPAAAPHRLLLSVHGGYGFSSYQQIDTEPPASGSGMPPLAQTLRRGALQAGVDAW
                     YVFSGYLGLGITAGLVSARNSESLNAQGSQISYDSNVRYLSITAAPAAAFSLLGNLGA
                     PGSHDLRLELSSGYSYNTANMELSLRNTPGNFPTAATASGYKHALATEIRLGYLAPLT
                     DNLRLKIAIAYLRIFHTGIYSSQLQGNVPFPNGDGFKRDFDARLSAEAANPQVLTAQL
                     GIEYGF"
     gene            complement(5335..>5721)
                     /locus_tag="PLF85_11275"
     CDS             complement(5335..>5721)
                     /locus_tag="PLF85_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20292.1"
                     /translation="AFTVKITAQGSEVNLTEGRIRIIRYHSNTGKWQTSEVASPYSGL
                     FDVKPVESVPEAVAEPEPEVIKPIVQKPRSRFSQYVGKSITLELKNGDRLTGTLRAVS
                     GAKLRLLTASGELNVPEAQVQRVQSN"
BASE COUNT         1297 a         1561 c         1527 g         1336 t
ORIGIN      
        1 tctcattgaa cttttttttc gtcttttacc cctcaatggt ctcatcgtcg gaaattttga
       61 gacccgtgag catttttttg gtttttcggg caatcctggc agtgtgtcgt attatgtcac
      121 atcaccggca aagggccagg gccattgccg agggtaaatg cggggtcagg gctgggattt
      181 gccaaccctt agagagcctg aatcgtaacc gaaatagctc ccacagctta ggcggagcgt
      241 ttggtctgct cgacgatgta ctggtaaagg agtatctgcg cttttttgaa ctggaccttt
      301 ttgtcctgga tcagcttttt cgagatggcc agggcttcgg cgttcttttc acggcagagt
      361 ttggcgaact tggcgtcttc gatgacgatg tgtgacagca gccattcttt cagatcatgc
      421 acgagcaccg atgcgccctg ccgcgtgcgc gttgcatagt cagcgtcgtg agaagtcacc
      481 gtgcgcacaa agagcgcgtg ctggttcttg tgcccggcgc gcaccgggta atcgagcgca
      541 tccatcatcg cttcttcggc ggcaaaatgt tcgagtatgt actgcttcac atcgggcaac
      601 aggtcggtga gcaggctcgc acgcttatcg tagtcaacct tgagcgcctc gctcagctcg
      661 accaccatct tgatcagcca gaggtgctgc atgtcgatca gcggcacgta gagcctgatc
      721 gaaaaactgc gccacatgcg ctgtatctcg tgcagcactt cattgctgat accggggata
      781 atttcgttgt gctgcgagat cgcagcatag aggtcgagct ggttctgatt gagggcgtac
      841 tctgaaccgc cggccacgag gcgctggaaa taggtgttcg cgtcgatatt gttctggcgg
      901 aagaagtcgc ggtacgacat gtcctctttc ttgatatgct gaatcagcca gttctgcaga
      961 aatcggttga gcttgccggc attctcgccg gtttcgttgt cgggcgaaag cagcaggttg
     1021 cggatattct cctgaaaggt gtggtgttgt gccgcgtggt tatcgacgtc gggaaagcgg
     1081 tattcggcca acaccatctc ttctgccgcg aaatgtgtgc gcgcatagcg cagcgcttcc
     1141 tggaaaacgc cttctacctg cttgtcatct cgcttttgcg ggtctttgat caatgactcc
     1201 aggtgaaaaa ccagtgcaat cagccacatg tgctgggcat cgagcatttc aataccgagc
     1261 gtgaagtcgt gctcgcgcca gaacttgcgc accctgttca gggtttcttc ggcgatggta
     1321 atgtgcaggt cttcgatatc gaccttgaga gaagtcattg gcaccctccg tggctggcag
     1381 attcttccta taagtgaaac accgcgtcaa caaattatgg atatttttat ccactatatg
     1441 aagccttgcc agcccggttt ttttggcttg cggccggcga cctgcgacgg cttcgtgcac
     1501 tcactatgct taagaaactt caccataaag tcctggctcc tgcagggggc attgccctgc
     1561 tctttttcac gttcctgccc gtttcggcac aggatctacc cgctgacccg gcggctgacc
     1621 aggtcgaaaa agacctgaga acgaactacg cgcgcaagta ccgcggcagc aatatcaccg
     1681 aactgagttc ggcaggtgaa ggtgtgttgc tagagaaaaa gaatgccaag ggcaaaaccg
     1741 tcgaaatcaa gcgcaaattc ccgttcaacg tttctgccac caagggcaaa aaacagttcc
     1801 agtacgaaat cggcgtcaac tacaagctcg tgaagggcaa gtgggtcttc tctgaactcg
     1861 gtgtcggcgc agccaaagaa attgccgctg ccgggcaact ggcgccggcg aaggcagaag
     1921 tgaagaagat gtttgctgaa gctgtgagcg ctaaattctt caataaccag ccggtcgatg
     1981 gtgtcttgct caccgacccc gattttgccg gcggcgctta ccgctacaac gcggagttca
     2041 aggtcggcgg ttctcagacc tgtactgact gggatttcat tctgaggcag gatggtggct
     2101 catggaaagt ggccgacatc atgcgcggtc gctgtaacta atcttagcag gcaccgcaac
     2161 gcggtgccgt acaaaatttg ccctaaagat gactcaagct gatctgaaat ttctgaaagc
     2221 gctcgcgaag aataatgacc gcgagtggtt caacagccac aaatccgaat tcgaaacggc
     2281 acgcgacaac tttcttcacc tggtgagcgc attcatattc ggcatgggcg acatcgacga
     2341 aagtattgcg ggcgtagaac ctgcgagctg ccttttccgt atttaccgcg acgtgcgctt
     2401 cagtaagaac aaggcgcctt acaaaaccca cctcggcgcc ttcatcagca gcgccggacg
     2461 caagggtttc aatacacccg gttattacct gcatattgag cccggcggcg aatcggtctt
     2521 cggcagcgga ctctatatgc ccgataaaga ccggctcgca gcggtacgca gggaactcag
     2581 cgctccaaat agcagggtag cggcggtttt cgccgacaag aaattgcgtg cggcatttcc
     2641 gcaggtactc gaagacgaca aggttacgcg tgtaccacgc ggttttgacc cgcaatcgcc
     2701 tcgcgcagaa ctattgaagc tccgccattt ctcggtcttt gcccgcctac ccgacaccca
     2761 ggtgacgaat cggaaacttg tacagcaact gctcagcaaa gcccctctgc tcaagcactt
     2821 caacgatacc ctcacgcagg cgacgcgcac gcgctgatac gaaactctgc acggcggagg
     2881 catacaattc cgcttgaccc tgccgtgctg acggttcagg aagctccatg cccactcatg
     2941 cgctgaagag aactttgttc gccctcctcc ttgtttcact ggctcctgcg ctttttgcct
     3001 ggaacaaggc agatgacatt ccagttgaaa atctgccggc agaggtgaag tcggtgcttg
     3061 aaaaatatgt gcaaatactc aaagagagcc catcggtcga tgaagccgcc gcggcttttg
     3121 cacagatcgc cggcggttcg ctggtcaatg acaacggcag tgtttcaaat aatacccggc
     3181 aatttggcct aaagaaagac ttcaacaatt tgaagcacta tgcgtggccc attcggatta
     3241 cccgcgtgaa caagactgtc tcgcgcggtg aaggttatgg cgcaaggaaa attgccggcg
     3301 atcgctatac gatctggatt gcgaaagatg atcccgcgaa ggggctgccc gcgcccgtca
     3361 cgatcatagc tcccactggc ggcgaaccga aagttgtcaa cgtcggcagc tactgaacgc
     3421 tgtgcaattc tcttgacagg gccaattcgt gtctatggta gcacgatgct acgccgttac
     3481 cgccttttca ttctttcact gcttttgcta ccactcgttt cgtgtgcgct cttgcagggc
     3541 cgcttcagcg agtttacgcc cgatattcgc cttaaagagg taaacctgcg gggtttcgac
     3601 tttgaaggtg cagaccttga atacgtctat accataaaga acaagatcgg cttcggcatc
     3661 acgttttcac ggctggcctt ccagatcgcg gttgatggca aacgcatggt cgacgtcaaa
     3721 aatgacaaga atgttgtcat caaggcgaat gacagtactg aattcacgat cgtccagcgt
     3781 gtgcggtacg tcgaaacggt agaagccatc ttcgaatttg ccaagaaaga ttctgtggcg
     3841 attgcgctga ccggagcagt cggtgtctac attaatgaac tgatcggaac gatcgaagtc
     3901 cccattgaag cgagcaagac cgtgccggta cctaagctgc cccaggttca cttcggctcg
     3961 ctcgactttg agcgcatgaa cctcagtaac ccgctgaacc cgcaggcaac gttcacactg
     4021 cgtttcaacg tacgcaatcc caacccgttc gaggttaaaa taccgaaagt cgattacgct
     4081 ttcaccgcgg ccggtacgaa cgtcatcagt ggggcacgcg ctaaccagac gctcaaaccg
     4141 caggctgact cgctcattac agtaccggtg aacctttctg gccgcaatat tgttgaattg
     4201 gtgccaaaac tgcgcgatct caattcaacc gactaccgct tcacctcggc ggttgaattt
     4261 gcgataatgg ggcaaagcgt cagcctgccc ttctactacc cgaaataatc agaagccgta
     4321 ttcaatcccc agctgggctg tcagtacctg ggggtttgcg gcttcggccg acaaccgcgc
     4381 gtcgaaatcc ctcttgaagc catcgccgtt gggaaacggc acattaccct gcagctgcga
     4441 cgaatagatg ccggtatgaa aaatccgcag gtacgcgatc gcgattttca ggcgcaggtt
     4501 atctgttaaa ggtgcaagat aaccaaggcg gatttctgtc gcaagcgcgt gcttgtagcc
     4561 actggcagtg gcagccgtag gaaaattacc cggcgtattg cgcagtgaga gctccatatt
     4621 ggctgtgtta taagaataac ctgacgagag ctcaaggcgc aggtcatggc tgcccggtgc
     4681 accgaggtta cccaacagag aaaatgccgc ggctggcgcc gctgtaatag aaaggtaacg
     4741 gacattactg tcgtacgaaa tctgcgaacc ctgcgcattc agtgactcgc tgttcctggc
     4801 gctgaccaaa cctgcagtta ttcccaggcc aaggtacccg gagaacacat accaggcatc
     4861 gacacccgcc tgcaaagccc cgcggcgcag agtctgggcg agcggcggca tacccgaacc
     4921 cgatgcaggt ggctcggtat cgatctgctg gtacgatgag aatccgtaac caccatgcac
     4981 cgagaggagc aaacgatgcg gcgctgccgc aggagttgac tgcacctgct gctgccggat
     5041 agtcttgccg tcgtcggtca gataaacttt cgtgttctca tcttcggaaa ccagtcgtgc
     5101 ctgcacaacc ttgttgtcat gactgatgtt tttgagctcg agagtttttt catcgagaat
     5161 cagccgactg acgtcactct tctttatgcg caactggccg tacgggctgc gaaaaatata
     5221 gcggtcgttt ttctcctgca ggatagtgcc agtgatcaca tcgccgttct tctgtacgat
     5281 caccgtagcc gaaaccggcg agagcgcaag caacaggaat agccgccgca taagttaatt
     5341 gctctgcacg cgctgtacct gcgcttcagg cacattcagt tcacccgacg cagtcagcaa
     5401 cctgagtttt gcaccgctca ctgcacgcag tgttcccgtc agtcgatcgc catttttcag
     5461 ttcaagtgtg atactcttac cgacgtactg gctgaaccgc gatcggggtt tttgtacgat
     5521 gggtttgatt acctctggct cgggctctgc cactgcttca ggcacagatt cgaccggctt
     5581 cacgtcaaac aagcctgaat acggtgaagc cacctcgctg gtctgccatt tgcctgtgtt
     5641 ggagtgataa cggataatgc gtatccttcc ctcggtgaga ttgacctcgc taccctgtgc
     5701 agtgatcttt acggtaaatg c
//
LOCUS       DAOQJF010000212         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8117_length_5705_cov_4.666549, whole genome shotgun sequence.
ACCESSION   DAOQJF010000212 DAOQJF010000000
VERSION     DAOQJF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8117_length_5705_cov_4.666549"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLF85_11280"
     CDS             complement(<1..608)
                     /locus_tag="PLF85_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20293.1"
                     /translation="MSDTDAKNHRSGDSASLDTPMMRQYLEIKKQYPDDILFFRMGDF
                     YEMFLEDAVYASRVLDIALTRRQDKVPMCGVPHHSMSQYVHPILEQGRRIAICDQLED
                     PAQAQGRIVRRGVTRILTPGSLFEEALIDGKESRRLCGISQTKNFLQLAIIEVATGQI
                     WFERKSAETLGEAATAFSIREWVVSDADDLSGRFDSAQRPEAV"
     gene            complement(643..1311)
                     /locus_tag="PLF85_11285"
     CDS             complement(643..1311)
                     /locus_tag="PLF85_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNE20294.1"
                     /translation="MLVKICGLTSVQDARLAAGAGADFLGIVMAESSPRRATAENARA
                     ILQAAFTQRIYLVFGYDDADYIRETFTALASSTTALQIMADHPQIDQLLKLAPTNRIM
                     PSISAAEKVTAAQISRWEQHPLVLFDSHRKSRDFGGAATTIAGGTGKTFDPANVAGIT
                     RPYLYAGGLNPDNVAGIVAKVNPPGVDVASGTEASPGIKDGAKVRAFIQNAKRAAQMI
                     EVQQ"
     gene            complement(1311..2870)
                     /locus_tag="PLF85_11290"
     CDS             complement(1311..2870)
                     /locus_tag="PLF85_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HNE20295.1"
                     /translation="MRLLLAVALFSCHKHSYDIEPDTIRFHLPQDPILLNPVISEDAY
                     SNVVCSRIFESLLERDRKTLKMKGMIAEKWTIGGDKLTYTFKLRRNIVFHDGKPLTAA
                     DVLFSYEMMMSEKIPNAHKKVYYKDVQSVAAPDAYTVVFRMKKPYAMALEHLGGFEVI
                     PKHIYSQGDFMKDERNLRGPIGNGPYRFVEWKTGQRVVLTRFDDYWGERPEIRKLEFT
                     IIKNDAVALQALKKGDVDSYNLRPLQWTRQTSSERFARDFEKIKYLATSYRYIGYNMR
                     REPFNDRRVRTAMAHLMDLERVKRTILEGLVEVTTGPFLPQSLQYNKKLKPLEFNPEK
                     AVALLLEAGYARDANGMLAKNGKPLEIELMFPAGGGFADQLVSVIKEDFSRVGITVQP
                     RKLEFQTMLTKINERDFQAVMLGWSSGIESDPYQLWHTSQKAKGHNFTGFGDAASDAL
                     IEQARLTFDDAQRNAIYHRFHERVYNDQPYTFLFTSYALVAVSKRFTNVNVYPLGLDL
                     LEWKLKKPLVQ"
     gene            complement(2939..3703)
                     /locus_tag="PLF85_11295"
     CDS             complement(2939..3703)
                     /locus_tag="PLF85_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE20296.1"
                     /translation="MIETRVENHIGYLAMNRAPMNLMDIPFMEEFMQAHRELAANDDV
                     WGVICHSTVDGYFSNGLDPAMMLSRDPKGRIEVFEVLLKMVLEVYAFPKPHISAIEGH
                     AMAGGAVIAILSDWRFMSRERSRISFSEVAVGLTIPKAIINLIETATGPGPLREIAML
                     GSAIKSTDAQRMGLVDEVFDNGTTLKEAEKYMKRLFDLPLASVRSVKQILRADNIAAL
                     KSRDGLETLGHFVGAESFVEGLSAVRDKRRPKYKNP"
     gene            3817..4392
                     /locus_tag="PLF85_11300"
     CDS             3817..4392
                     /locus_tag="PLF85_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20297.1"
                     /translation="MYGHLLQRKIHDLLRIERGSWYPAFVGCIAFAASVTMTIPFAGP
                     LVIAVLFKPKRWKQLVIASSVGSALGATTLLMIFHHGAWAAIMHWHPHISGGASWIAL
                     REWLEAYGTLSLFIIMALPVPDSPALLFFALHQGNPLPVLLATFAGKFIKYSVYAFLV
                     KTFPERFHLSTLLHLSQQAGTDGPVGRVPGS"
     gene            complement(4389..5066)
                     /locus_tag="PLF85_11305"
     CDS             complement(4389..5066)
                     /locus_tag="PLF85_11305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE20298.1"
                     /translation="METMFRLTKPTAADVLFDLGCGDARILVAAARRFRTRGVGYDID
                     TRLLDKGRNDAQKNRVAELITLRAEDLFAADLSSATIVAIYLSDRINSKLRPKFFREL
                     RPGTRIVSHNWHMGDWLPDARVFTQDRTRVVYYWVIPANFSGRWIENTGTALTIRQHY
                     QVAEISLEGAPVGAKQQEYRVDAFRLRHPELGILELSGETLSVTKRGSPTRLFHRQPG
                     TMEPPAI"
     gene            complement(5066..5557)
                     /locus_tag="PLF85_11310"
     CDS             complement(5066..5557)
                     /locus_tag="PLF85_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20299.1"
                     /translation="MLRRIFTLSLVTMFAAGCMSGNRKKSDLAEPTRGFWFLSEKFPY
                     QFLRVELYPDHVEGLPSLAMEFKIRAESQGMTWSTTGEQVVIRFVRPNPGRVTFTPVT
                     DRKTYRGRYAREALQLPLVEVKIGGERLVMHYTESEAEMDRLLRQKNKLRLDVVWGPL
                     PPK"
BASE COUNT         1108 a         1557 c         1681 g         1359 t
ORIGIN      
        1 actgcctcgg gtcgctgagc ggagtcgaag cgccccgaga gatcatcggc gtcgctcacg
       61 acccattcac gaatgctgaa tgccgttgcc gcttcgccga gcgtttctgc agatttgcgc
      121 tcgaaccaga tttgccccgt ggcaacttcg atgattgcca gctgcaggaa attcttggtt
      181 tggctgatac cgcacaaccg gcgcgattct ttgccgtcga tcagcgcttc ttcaaaaagg
      241 ctgcccggag tcagaatacg cgtcacgccg cgacgcacga tgcggccctg cgcctgcgcc
      301 gggtcttcga gctggtcgca gatcgcaatg cgcctgccct gttcgagaat cgggtgcacg
      361 tactggctca tcgagtggtg cggtacgccg cacatcggca ccttgtcttg ccggcgcgtc
      421 agcgcaatgt cgagcacgcg cgaggcatag acagcgtctt ccagaaacat ctcatagaaa
      481 tcgcccatgc gaaagaacaa aatatcgtcg gggtattgtt tcttgatttc gagatactgc
      541 ctcatcatgg gagtgtcgag actcgcagag tctccgcttc ggtgattctt ggcgtcggtg
      601 tcgctcacgc gccacctccg caagaatctc gcgatcccgc tttcattgtt gcacctcgat
      661 catctgtgcc gcccgtttgg cgttttggat gaacgctctc actttcgcgc catctttgat
      721 tcccggtgat gcctcggtgc cgcttgccac gtcgacaccg ggtggattga cctttgccac
      781 aatgccggcc acgttatcgg gattgagccc acccgcgtag aggtatgggc gtgtaatgcc
      841 ggccacattc gccgggtcga aggtttttcc tgtgccacct gcgatagtgg tggctgcgcc
      901 accaaaatcg cgactctttc tatgtgaatc aaacaacacg agagggtgct gctcccagcg
      961 cgagatctgg gctgctgtga ctttttctgc cgcggagatc gagggcatga tgcggttcgt
     1021 cggcgcaagc ttcagcagtt gatcgatctg cgggtggtcg gccatgatct gcagcgcggt
     1081 ggtcgagctc gccagcgcgg tgaaagtttc gcgaatatag tcagcgtcgt cgtagccgaa
     1141 gacaaggtag atgcgttggg tgaaggctgc ctgcagaatc gcgcgggcat tttctgctgt
     1201 ggcgcggcga gggctcgact cagccatgac aatgcccaga aaatcggcgc ccgcgccagc
     1261 cgcgaggcgt gcatcttgca cagaggttaa tccgcagatc ttgacgagca ttattgcacc
     1321 agtggctttt tgagcttcca ttcgagcaga tcgaggccga gcgggtagac gttcacgttg
     1381 gtaaatcgtt tgctgacggc gacgagagcg tagctcgtga agagaaaggt gtagggttgg
     1441 tcgttgtaga cgcgctcgtg aaaccggtgg tagatcgcgt tacgctgcgc atcgtcgaat
     1501 gtcagtcgag cctgttcgat cagtgcatcg ctggcggcgt cgccgaaacc cgtgaagttg
     1561 tgacccttcg ccttttgcga agtatgccac agctggtagg ggtcagactc aatgccagac
     1621 gaccagccga gcatcaccgc ctggaaatcg cgctcgttga tcttggtcag catcgtctgg
     1681 aactcgagct tgcgcggctg caccgtaata ccgacacggg agaaatcttc tttgatgaca
     1741 gaaaccagct ggtcggcgaa gccaccaccg gcggggaaca tcagttcgat ttcaagcggc
     1801 ttgccgtttt tggcgagcat gccatttgca tcgcgtgcat agcctgcttc aagaagcaac
     1861 gctaccgcct tttcgggatt aaattcgagt ggtttcagct tcttattgta ctgcaggctt
     1921 tgcggcaaga acggcccggt cgtgacttcg accaggcctt cgaggatggt acgtttcacg
     1981 cgctccaggt ccatcaggtg cgccatcgcg gtgcgcacgc ggcggtcatt gaacggttcg
     2041 cggcgcatgt tatagccgat gtagcgatag ctggtggcga ggtacttgat cttctcgaaa
     2101 tcgcgcgcga atctttctga actggtctgc cgtgtccatt gcagcgggcg caggttgtag
     2161 ctgtcgacat cacctttctt cagagcctgc agggccacgg catcgttctt gatgatcgtg
     2221 aattcgagtt tgcgaatctc gggtctttca ccccagtagt cgtcgaagcg cgtcagcacg
     2281 acgcgctggc cggttttcca ctcgacgaaa cggtaggggc cattgccgat cgggccgcgc
     2341 aggttgcgct catctttcat gaagtcgccc tgcgaataga tatgcttcgg tattacctcg
     2401 aacccgccga ggtgctcgag cgccatggcg tatggcttct tcatgcgaaa aacgaccgtg
     2461 taggcgtcgg gcgctgcgac cgattgtaca tctttgtagt aaaccttctt gtgcgcattg
     2521 ggtatcttct cgctcatcat catctcgtag ctgaagagca cgtcggctgc ggtaagcggt
     2581 ttgccgtcat gaaagacgat attgcggcgg agcttaaacg tgtaggtgag cttatcgccg
     2641 ccgatggtcc atttttccgc gatcataccc ttcatcttga gcgtcttgcg gtcacgttcg
     2701 agcaggcttt caaagatgcg gctgcacacg acattcgaat acgcatcttc agagatgacg
     2761 gggttgagca agatcgggtc ttgcggcagg tgaaagcgga ttgtatctgg ttcaatatcg
     2821 tagctgtgtt tgtggcagga gaatagcgca acggccagca gcagcctcac cccccagccc
     2881 cctctcctta aggagagggg gaggagcgaa gcggaggggg agaggctgga cggcttcttc
     2941 atggattttt atacttcggc cggcgtttgt cgcgcacggc tgagagaccc tcgacaaaac
     3001 tttccgctcc cacgaagtgg cccagggttt cgagcccgtc gcggctcttg agcgcggcga
     3061 tgttgtcggc gcgcaagatc tgtttcacgc tgcgcaccga agcgagcggt aggtcgaaga
     3121 gccgtttcat gtacttttct gcttctttca gggtggtgcc attatcaaag acttcgtcga
     3181 cgagccccat gcgctgcgcg tcggtcgatt tgatcgcgct gcccagcatt gcgatttcgc
     3241 gcaaggggcc ggggccggta gccgtttcaa tcaggttaat gatcgctttg ggaattgtta
     3301 atcctactgc gacttctgaa aacgaaatgc ggctgcgctc gcggctcatg aagcgccagt
     3361 cagataatat ggcaatcacg gcgccgccgg ccattgcgtg gccctcgatg gccgagatgt
     3421 gcggcttggg aaatgcatag acctcgagca ccattttcag cagtacttcg aacacctcga
     3481 tgcggccttt ggggtcgcgg ctcagcatca tcgcagggtc gagcccgttt gagaagtagc
     3541 cgtcgactgt cgaatggcag atcactcccc agacgtcgtc gttggctgca agctcgcggt
     3601 gcgcctgcat gaactcttcc atgaacggga tgtccatcag gttcatgggg gcccggttca
     3661 tcgcgaggta gccgatgtgg ttctcgacgc gggtttcgat catgatttgt atcctgcgag
     3721 catggcacaa aagaagcaaa acaaacatgc ggacaaggaa aagtcaggcg gatgatttgg
     3781 tttgacgaaa ccatgaggta tgcttaggag agcaatatgt acggacattt gctgcagcgc
     3841 aagatacatg atcttctgcg catcgagcgt ggcagttggt atcctgcttt tgtaggctgt
     3901 atagcctttg ccgcttcggt cacaatgaca attccatttg ccgggccatt agtcattgct
     3961 gtgctcttta aaccgaaacg ctggaagcag cttgtcattg cttcttcggt tggctccgcg
     4021 ctgggagcga cgacgctcct catgattttt catcacggcg catgggctgc tattatgcac
     4081 tggcatccgc acatttcagg cggagcatcg tggatcgcac tgcgcgagtg gcttgaggct
     4141 tacgggacac tgagcctgtt tattatcatg gctcttcccg tgccggatag tccggcgctg
     4201 ttattttttg ctcttcacca gggtaatcca ctgcccgtgc tcttggctac cttcgccggc
     4261 aaattcataa agtattcggt ctatgctttt cttgttaaga catttcccga gcggtttcat
     4321 ctgtctacgc ttctgcacct ttcccagcaa gccggtacgg acggcccagt gggtcgtgtg
     4381 cctggaagct aaatagccgg aggttccata gtccctggct gtcggtgaaa taaacgcgtg
     4441 ggacttcctc gttttgtgac ggatagggtt tcgcccgaaa gttcgagtat ccccaattcg
     4501 ggatgccgaa ggcgaaaagc atcaacacgg tattcctgct gttttgcacc gacaggcgct
     4561 ccttcgagac tgatttcagc gacctggtag tgttgccgaa tcgtcagagc agtgcctgtg
     4621 ttttcgatcc atctgccgct gaagtttgcc ggtataaccc agtagtaaac gacgcgggtg
     4681 cggtcttgcg tgaatacccg cgcatcgggc agccagtcgc ccatgtgcca gttgtgcgag
     4741 acgatgcgcg tgccgggcct gagctccctg aagaacttag gcctgagttt cgagttgatt
     4801 ctgtcgctca ggtagatcgc gacgatcgtc gccgaagaca ggtctgccgc gaacaggtct
     4861 tcggcgcgca gggttatcag ttcagccacg cgatttttct gtgcatcgtt acgccctttg
     4921 tcgagcagcc tggtatcgat gtcgtagccg actccgcggg tgcggaaacg ccgggctgcg
     4981 gccaccagta tgcgcgcatc gccacagccg aggtcgaaga gcacatctgc ggccgtgggc
     5041 ttcgtgagcc ggaacatcgt ttccatcact tcgggggtag tggaccccag accacgtcga
     5101 gccgcagctt gttcttctgt ctcagcaggc ggtccatttc ggcttcgctt tcggtgtagt
     5161 gcatcacgag gcgttcgccg ccaattttga cctcgaccag gggcaactgc agcgcctcgc
     5221 gcgcgtaacg gccccggtag gttttgcgat ccgtcacagg ggtgaacgtc acccggccgg
     5281 ggttcggcct gacaaaacga ataactacct gttctccggt ggtactccag gtcattccct
     5341 ggctttctgc gcgtattttg aattccatcg ccaggcttgg taaaccttcg acgtggtcgg
     5401 ggtagagttc gacgcgcaaa aactggtagg ggaatttttc ggagaggaac cagaagcctc
     5461 tagttggctc tgccaggtcg gatttcttgc ggttacccga catgcaaccg gctgcaaaca
     5521 tggtgacgag actcaaggta aagatgcggc gtaacattgg acatcagcag gccggtacct
     5581 gatactcagt gaggcaacca taaagtggtt tgcgccccga tgagcgccta gaatcggctc
     5641 tctagcaggg gtatttcccc gttatcaata atcgttgagg ccaaatggca gacaccacat
     5701 ccaaa
//
LOCUS       DAOQJF010000213         5701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8128_length_5701_cov_5.193943, whole genome shotgun sequence.
ACCESSION   DAOQJF010000213 DAOQJF010000000
VERSION     DAOQJF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8128_length_5701_cov_5.193943"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLF85_11315"
     CDS             <1..871
                     /locus_tag="PLF85_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE20300.1"
                     /translation="GSIDTFATAKLVSILVTNSVFASDVINGRAKIKNPPLPLITVPV
                     EPSLGEELCPSFTMRDAADGQKKYFEHELLFPVATFYDPKVCEHLTSDSAARLGGATL
                     AYAIESQLSPKSNPISSALALRAIELMRRNIPQLYKDAKNDKLISTMLWSSAMISISA
                     VSSPVGVAYSIANALNAHDKVNFYDALSLILPHVMEYYLTAAPAQYINIARALGEDVK
                     DISVIEAAIKAVEGVRRLFLEVNLPTRLSEFDVKKHELEDISRLAAVFPHLENAPRML
                     TRNEIESILLAAY"
     gene            927..1703
                     /locus_tag="PLF85_11320"
     CDS             927..1703
                     /locus_tag="PLF85_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20301.1"
                     /translation="MPLYANAPDRAFCEKILGENKRFIDFINPGVSNFAGEEHLKIFR
                     SAALEHYKAHKEYLSGHYKETHAHIRESQKILKDLYLQVLNDYYQKDTRELLERNSAM
                     IVETKDVRAAHFMRLGYRDLKVSEIYEGKGYNHNKHLYSTKIWFYIDAIKYARQAKRY
                     AFLAIIESQTPYADKDDFKIQTIDDYFNKPEVEKQRDFDRVQSRLINLINRKLVVNNY
                     DFFFHHYDNYGYINSGKKNQYEEFVMAQGSESLQPKKPRP"
     gene            complement(1717..2769)
                     /gene="lpxD"
                     /locus_tag="PLF85_11325"
     CDS             complement(1717..2769)
                     /gene="lpxD"
                     /locus_tag="PLF85_11325"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002180173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNE20302.1"
                     /translation="MKLTEIAASLGGQVKGNGNLEIQGVRDLELIGGQGRDTAPQAGY
                     IYYVESKKVFNQHPESVHAEALLTTKTLSGNFANAVVVEDAQARVAFIRLLAMFEQVP
                     APVAAEKGAAYIAPDARVDASAIVMHGAVILPGAEIGANAVIHPNAVIEQNVKIGAHT
                     VIHPNVVIKFNCVIGSHCIIHSSTVIGADGFGFYDKDGVRYKVPQIGNVIVGDHVEMG
                     ASVTVDRATIESTVIGDYTKFDDQVHVGHNCQVGKYVYIAGNTVLAGSVTLEDNVTLG
                     GQTAISGKVLVRKGSFVMGMSALAADSEPGGMYFGIPARPAREMHKINGSLVYVPELL
                     KRVAALEEKLGLEKNS"
     gene            2946..3137
                     /locus_tag="PLF85_11330"
     CDS             2946..3137
                     /locus_tag="PLF85_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20303.1"
                     /translation="MQNENHGRIIDMAKAFTPAVRAQNANNSTGRQDGKALIMEPRTI
                     RHQERVEDLFYLLFRRTGA"
     gene            3184..3471
                     /locus_tag="PLF85_11335"
     CDS             3184..3471
                     /locus_tag="PLF85_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNE20304.1"
                     /translation="MIQVNEYFEGKVKSLTLQANGNKATCGVMAAGEYEFSTGLKEIM
                     TVVAGAMTVRLPGSSDWQTFAAGQKFEVAANSKFQLKIPTDAAYLCEFIPG"
     gene            complement(3509..>5701)
                     /locus_tag="PLF85_11340"
     CDS             complement(3509..>5701)
                     /locus_tag="PLF85_11340"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766994.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNE20305.1"
                     /translation="GLQFHTTINEWHTCPEDGRINASNPCSEYMFLDDTACNLASLNI
                     MKFYDASKNQLDVEAYKHAIRIWTIVLEVSVLMAQFPSQRIAELSYKFRTLGLGYANL
                     GTWLMVSGIPYNSAQANAITGAVTAILTGVSYKTSAELAAELGPFAGYEKNREHMLRV
                     IRNHRRAAYNVAESEYEGLSIFPLGINGKYCPEYLIGDAKQAWDEALALGEKHGYRNA
                     QTTVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINKSVPQALEHLGYSK
                     DQISKIVEYVVGSASLAGAPHVNRENLKLRGFTDEILDTIEDALPTAFDINYVFNRYL
                     VGDAFLTGTLKIPKEVLDKPDFNLLQHMGFTAEQVMLANEAICGTMMIEGAPYLKEEH
                     LPVFDCANKCGRRGKRFIPYQAHLNVMAAAQPFISGAISKTINMPYEATVEDVKQAYM
                     ASWKLMIKANALYRDGSKLSQPLSTVGENIFEDLDEDDIADVMSAPAALKAQQTAEKI
                     VEKIIVRKIAERRRLPARRQGYTQKAVIGGHKVYLRTGEYEDGSLGEIFLDMHKEGAA
                     FRSLMNGFAIAVSLGLQYGVPLEEFVEAFVFTKFEPNGVVQGHDRVKMATSVMDFIFR
                     ELAITYLERNDLAHVSPEALVVDQTTTKGEVPGADALDDHEEPVAMAVGETKTRSGLK
                     VKVSASQIQEARIKGYEGSACPECSSMTLVRNGSCLKCETCGATTGCS"
BASE COUNT         1367 a         1604 c         1425 g         1305 t
ORIGIN      
        1 cggttcgatc gacacatttg ccacagcgaa gctcgtttcg atactcgtga ccaattcggt
       61 ttttgcctcc gacgtgatca atggccgggc caaaataaaa aatccccctc ttccgctgat
      121 taccgtgccg gtcgaaccat cgctgggtga agagctctgc ccgagtttta cgatgcgcga
      181 cgccgccgac gggcagaaga aatacttcga gcacgaactg ctctttcctg tcgcgacgtt
      241 ctacgacccc aaggtctgcg aacacctgac ctctgacagc gctgcccgcc tcgggggagc
      301 gacccttgct tatgcgattg agagccagct gtcgcccaag tcgaacccga tttcgagcgc
      361 gctggcgctg cgcgctattg aactcatgcg ccgcaacatt ccgcagctct ataaagatgc
      421 caagaatgac aaactgattt cgacgatgct gtggtcgtcg gcgatgattt cgatctcagc
      481 agtctcatca cccgtaggcg tggcttactc gatagccaat gcgctcaatg cgcacgacaa
      541 ggtgaatttt tacgacgcgc tctcgctcat tctgcctcac gtcatggagt attacctgac
      601 cgctgcaccg gcacagtaca tcaacattgc ccgtgcgctc ggcgaagacg tgaaagatat
      661 ctcggtcatt gaagctgcga ttaaggcagt cgagggtgtt cgccgcctgt ttctcgaggt
      721 gaacctgcca acgcgcctct cagaatttga cgtcaagaaa cacgaactcg aggacatctc
      781 gcgccttgcg gcggtctttc cacacctcga aaacgcgccg cgtatgctga cgcgtaacga
      841 aatcgaatct attctcctgg cggcgtattg atatgaaagt gtttctaaac acacacaaat
      901 attgcgcagc aatatttatg tgtgttttgc cgttatatgc caatgcgcct gaccgcgcgt
      961 tctgcgaaaa aatactcggc gagaataagc gcttcatcga ctttatcaat ccaggtgttt
     1021 caaactttgc aggtgaagag cacctgaaaa ttttccgctc cgccgcgctc gaacactaca
     1081 aggcgcacaa ggaatacctg tcagggcact ataaagagac gcacgctcat atccgtgaat
     1141 cgcagaagat cctcaaggat ctctacctgc aggtactaaa cgactactac cagaaagaca
     1201 cgcgagaact actcgagcgc aattcggcga tgatcgtaga aacaaaagac gtgcgcgcag
     1261 cgcacttcat gcgcctggga taccgcgacc tgaaggtttc ggaaatttac gagggcaaag
     1321 gttacaacca caacaagcac ctctactcca ccaaaatctg gttctacatt gacgcgatca
     1381 agtatgcacg ccaggcgaaa aggtatgcct tcctggcgat catcgagagc cagacaccct
     1441 acgccgacaa agacgatttc aagatacaga ccatcgacga ctattttaac aaacccgagg
     1501 tggaaaagca gcgggatttt gatcgtgtac agtcacggct gatcaacctg atcaaccgca
     1561 agctcgtcgt taacaattac gacttctttt ttcaccacta cgacaattac ggctacatca
     1621 acagcggcaa aaaaaaccag tacgaagaat tcgtgatggc gcagggtagt gaaagcctgc
     1681 agcccaaaaa gcctcgtccc tgaattgctc tatagactaa ctgttctttt ccaaaccgag
     1741 cttttcttcg agcgcagcaa cgcgtttcag cagttcgggt acgtaaacca gagagccgtt
     1801 tattttgtgc atctcgcgtg ccggacgggc cggtatgccg aaatacattc cacccggttc
     1861 agaatcagca gcgagcgcac tcatacccat gacaaagctg cccttgcgca cgagaacctt
     1921 acctgaaatc gccgtctggc ccccgagcgt aacattatct tcgagcgtca ctgaacccgc
     1981 gagcacggta ttacccgcga tgtagacata cttacccacc tggcagttat gcccgacatg
     2041 cacctggtcg tcaaatttcg tatagtcgcc aatgactgtc gactcaatcg ttgcccggtc
     2101 aacggtgaca cttgccccca tttcaacgtg atcgcctaca atcacgttgc cgatttgggg
     2161 caccttatag cgcacgccat ccttatcgta aaagccaaag ccatctgcgc ctatcacggt
     2221 agaactatga atgatacaat gcgaaccaat tacacagttg aacttgataa ccacattggg
     2281 atgaattacg gtatgtgccc cgatcttcac attttgctca atcaccgcat tcggatgaat
     2341 aacggcgttc gcccctattt cagcacccgg caaaattacc gcgccgtgca tgacgatcgc
     2401 cgatgcatcg acgcgggcat cgggcgcgat gtaagcagct cccttttctg ctgccacagg
     2461 cgcaggtacc tgttcaaaca tcgccagcag ccgtataaat gccacgcgcg cctgtgcgtc
     2521 ttctacgaca acggcattgg caaaattccc ggaaagcgtc ttcgtagtca gcagcgcctc
     2581 ggcgtgcaca ctttccgggt gctggttgaa gactttcttc gattcgacat aataaatgta
     2641 tccggcctga ggcgctgtgt cgcggccctg acccccgatc aactccaggt cgcgcacgcc
     2701 ctgaatttcc agatttccat ttcctttgac ctggccgccc aggctggcgg caatttcggt
     2761 aagcttcatg cactgaggcg gacaagcccg tcgggagcgt caagcggaga ttttcttcat
     2821 gcccagtcag aattcaaaca gcagccaaca aaaaaacact cagcaaaatc aggcccgttg
     2881 ccgataatta agcaggcgcc gcctttgtct ggtgaaccgg caaaaaaggc gtaagaccga
     2941 tacctatgca gaacgaaaac cacgggcgta tcatcgacat ggccaaagcg ttcacacccg
     3001 ccgtgcgggc acagaacgcc aacaattcga ctggcaggca ggatggcaag gcgctgatca
     3061 tggaaccccg gacgatccgc caccaggaac gggtagaaga tcttttttac ctgctctttc
     3121 gccgcaccgg cgcttaagct ctcttcaggc gcgacgcgct gtcgcgtacc aaaaaacttc
     3181 cctatgattc aggtaaacga atacttcgag ggaaaggtaa aatccctcac cctgcaggct
     3241 aacggcaata aggcaacctg cggggttatg gcagcgggtg aatacgaatt ctcgactggt
     3301 ctgaaagaga tcatgacggt cgtagccgga gcaatgacgg tacggctgcc cggctcatct
     3361 gactggcaga ctttcgctgc cgggcagaag ttcgaggtag cggctaattc aaaattccaa
     3421 ctgaagatac ccacggacgc ggcgtatctc tgcgagttta ttcccggtta gtgtgaaatg
     3481 aggggtgctt tgcacccctc atttcatatc atgaacaacc ggttgttgct ccacaggttt
     3541 cgcacttgag gcacgaacca ttgcggacga gagtcatcga gctgcactca gggcaggcac
     3601 tgccttcata acccttgatg cgtgcttcct ggatctggct cgcgcttacc tttaccttga
     3661 ggcccgaacg ggtctttgtt tcaccgactg ccatagcaac cggttcttcg tgatcgtcga
     3721 gtgcatctgc accaggtact tcacccttgg ttgtggtttg gtcaaccacc agcgcctcgg
     3781 gagaaacgtg cgccaggtcg ttgcgttcga ggtacgtgat tgccagttcg cggaagatga
     3841 agtccatcac tgacgttgcc atcttgaccc ggtcatggcc ctggacgaca ccattgggct
     3901 cgaacttcgt gaagacaaaa gcttcgacga attcttcgag cggcaccccg tactgcaggc
     3961 ccagcgatac agcgatcgca aagccgttca tcagtgagcg gaaagccgcg ccctctttgt
     4021 gcatatcgag gaatatctca cccagcgaac catcttcgta ttcaccggtg cgcaggtaaa
     4081 ccttatggcc accgataacg gccttttgcg tgtaaccctg gcggcgtgcc ggcaggcgac
     4141 ggcgttctgc gattttgcgc acgataatct tctcgacgat cttttctgca gtctgctgcg
     4201 ccttaagagc agcaggcgct gacatcacat cggcaatatc gtcttcgtcg aggtcttcga
     4261 agatgttttc acccacagta gagagcggct gagacagctt agatccatcg cggtaaagcg
     4321 cgttggcctt gatcatgagc ttccaggaag ccatgtaagc ctgttttacg tcttcaaccg
     4381 ttgcctcgta aggcatgtta atcgtctttg aaatcgcgcc gctgataaac ggctgtgcgg
     4441 ctgccatgac gttgagatga gcctggtaag gaatgaagcg cttaccgcgg cgaccgcact
     4501 tattggcgca gtcaaatacc ggcaggtgct cttctttcag gtaaggcgca ccttcaatca
     4561 tcatcgtacc acaaatcgct tcattggcca gcattacctg ttcagcagtg aagcccatgt
     4621 gctgcagcag gttaaaatcc ggcttatcga gcacttcttt aggtatcttc agcgtaccag
     4681 tgagaaatgc atcgcctacc aggtagcggt taaatacgta gttaatgtcg aacgccgtcg
     4741 gtagcgcgtc ttctattgta tccagaattt catcggtaaa cccgcgcaac ttcaggtttt
     4801 cgcggttaac gtgcggagca ccggcgagcg atgctgaacc aaccacgtac tcgacaattt
     4861 tactaatctg gtctttgctg taaccgaggt gctccagcgc ctgcggcact gatttgttga
     4921 taatcttgaa gtaaccgcca cctgcgagct ttttgaattt caccaatgcg aaatccggtt
     4981 cgataccggt ggtatcgcag tccatcacga gaccaatcgt gcccgttggc gcaattactg
     5041 tcgtctgtgc attgcggtag ccgtgctttt cgccgagcgc aagcgcttcg tcccacgcct
     5101 gcttcgcgtc gccgatcagg tattcagggc aatatttgcc attaatgccc agcggaaaaa
     5161 tgctcaggcc ttcgtactca ctctctgcaa cattgtaggc cgcgcggcgg tggttacgga
     5221 tgacacgcag catgtgctcg cggtttttct cataaccggc aaaaggtccg agctcagcgg
     5281 ccagctccgc cgaagtctta tagctgacac cggtgagaat tgccgtcact gcacccgtaa
     5341 tggcatttgc ctgtgccgag ttgtacggaa taccggaaac catgagccac gtaccaaggt
     5401 ttgcataacc gaggcccagt gtgcggaact tatatgaaag ttcagcaatg cgctgcgaag
     5461 gaaactgtgc catcagcact gaaacctcga gtacaatcgt ccaaatacgg attgcatgct
     5521 tgtacgcttc gacgtctagc tggttcttgc tcgcatcgta gaatttcatg atgttgagcg
     5581 aagccagatt gcaggctgta tcatcgagga acatgtactc gctgcagggg tttgatgcgt
     5641 tgatacgacc atcttcaggg caggtgtgcc actcgttgat ggtcgtgtgg aactgaagac
     5701 c
//
LOCUS       DAOQJF010000214         5690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8150_length_5690_cov_5.087666, whole genome shotgun sequence.
ACCESSION   DAOQJF010000214 DAOQJF010000000
VERSION     DAOQJF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8150_length_5690_cov_5.087666"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLF85_11345"
     CDS             <1..838
                     /locus_tag="PLF85_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE20306.1"
                     /translation="MDAETLKKPPYDKGMGPWGAADERAMIQPLAEFASRDKNPFVAL
                     LFAFAPHHPYNMPDGFTETFGPEPGMKKSQVRYLNSLHFADQAFGEILAALESKGILR
                     NSILVVFGDHGEAFYEHPGNYNHPFFLYEENIHVPLMIYFDGVEAQHIARVTSHVDIL
                     PTVLDLLGKEKLRSPLHVGQSMLRGGAQSLAHLQAYWQEEYSGIVDAQFKYIRKETGA
                     HELFDLLQDAGEQQNLAQSRPELVTRYQSLTARAFAQKKRYYQKHAGYELTRFNPASQ
                     DK"
     gene            complement(873..2249)
                     /gene="nagZ"
                     /locus_tag="PLF85_11350"
     CDS             complement(873..2249)
                     /gene="nagZ"
                     /locus_tag="PLF85_11350"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNE20307.1"
                     /translation="MRKNRNLAIAALIVLISIAVAYALYDRIYLPRKYSALTEKIITG
                     AIAALDEDRLAGQPIHIAVPGTELTEETRKAIAAIKPGGIIFFGFNLQDAAQIKKLTT
                     DLQALAAELGLPPFLISTDQEGGYVRRVREGVLQTPPAMDMGATGDRELCRATGYHVS
                     RDLGALGINVFFAPIVDINNNPQNPVIGLRSFGANLDAVLGCALPFEQGARQAMREGG
                     ALPVIKHFPGHGDTQTDSHWALPVIDKKIDELRALELIPFREAIRDGAQAVMTAHILY
                     PQIDPDHPATLSSRWLSGTLRSELGFAGLVFTDAMEMNAVSQNFKTLDRPVTAILAGA
                     DVLLYTSWQEEPPEAHARLQAAIQSGTLKTGRNAESQPLPLQRALENQLRQKLALLDI
                     GKYLTPEEAAWYRKYTQALQAQKPATPVTLSKDDLTARFAQIRWSTKKKKGGPIWLAG
                     QKNSAKAR"
     gene            complement(2236..2406)
                     /locus_tag="PLF85_11355"
     CDS             complement(2236..2406)
                     /locus_tag="PLF85_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20308.1"
                     /translation="MTAGNLSYKEKAYSRGVGLIDLALICAARESRAKIWSRDRKMLS
                     LLKKEEVFHAQK"
     gene            complement(2474..2695)
                     /locus_tag="PLF85_11360"
     CDS             complement(2474..2695)
                     /locus_tag="PLF85_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HNE20309.1"
                     /translation="MKITALMDDELIHDVRTFSGGKNITESLVIALNDWLRIQKLRRL
                     NAEIEKAPLHFADSFSASSVRRLNQRRHP"
     gene            complement(2840..4552)
                     /gene="ilvD"
                     /locus_tag="PLF85_11365"
     CDS             complement(2840..4552)
                     /gene="ilvD"
                     /locus_tag="PLF85_11365"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002179668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNE20310.1"
                     /translation="MAKSAQKKKSDKRKFSAQVVDGVDRTPSRAMLRAVGFGDADFKK
                     PVVGVASTWSMVTPCNMHINLLANEAEKGVNAAGGKAVQFNTITVSDGISMGTEGMKY
                     SLVSREVIADSIETVVGAQGFDGFVTIGGCDKNMPGCVMAIARLNRPAVFVYGGTIMP
                     GKHSDGRDLDIVSVFEGVGAHANGRLNDAGLKEIEEKAIPGAGSCGGMYTANTMASAI
                     EALGLSLPNSSAQNAISAEKKDDCRRAGEAVLNMLQQGIRPSDILTRKAFENAITVVI
                     AIGGSTNAVLHLIAIAHTAGVKIGLDDFIRLGKKVPVLADLKPSGKYAMSRLIEIGGT
                     LPLMKMLLDRKLLHGDVLTVTGKTLAENLKNVKPYPADQDIVRSFDNPIKKDSHLVVC
                     YGNLAPGGSVAKISGKEGLYFKGKAKVFDREEDCLPAILNGKIKKGDVIVIRYEGPRG
                     GPGMREMLSPTSAVMGKGLGKDVALITDGRFSGGTHGFVVGHITPEAFDGGPIALIKN
                     GDEIIIDAKKNEVNLLVDKKELAKRKKAWKQPKPRYNRGILAKYHHQVQSASEGAVTD
                     KFGT"
     gene            complement(4578..5177)
                     /gene="leuD"
                     /locus_tag="PLF85_11370"
     CDS             complement(4578..5177)
                     /gene="leuD"
                     /locus_tag="PLF85_11370"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNE20311.1"
                     /translation="MEKFNTVKSIACPLDMPNVDTDQIIPKNFLKRVERTGFGQFLFN
                     DWRYKDDGSENKDFVLNFPQHKGARVLLARDNFGCGSSREHAPWSLLDYGFQVIIAPS
                     FADIFYNNCFQNGILPIALSAAEVEQLFQAVKNDAKTELTIDLEKKTISFDGKSIAFN
                     LAEPLRQKLLQGLDDIGWTLQFDAEISAHEKKYSAGIPA"
     gene            complement(5211..>5690)
                     /locus_tag="PLF85_11375"
     CDS             complement(5211..>5690)
                     /locus_tag="PLF85_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNE20312.1"
                     /translation="KDEVERVGIKKALEYMDFAEGTAIDQIKLDKVFIGSCTNSRIED
                     LREAAKVIKNRKVAANIKLAMVVPGSHRVKDQAEKEGLHRIFTSAGFEWREPGCSMCL
                     AMNDDVLEPGERCASTSNRNFEGRQGKGGRTYLVSPAMAALAAIHGHFVDARSEVTA"
BASE COUNT         1147 a         1599 c         1575 g         1369 t
ORIGIN      
        1 gatggacgcc gaaaccctga agaagccacc gtacgacaag ggtatgggac catggggtgc
       61 tgccgacgag cgcgcgatga tacagccact ggccgagttt gccagccgtg acaagaaccc
      121 ttttgtcgct ctgctcttcg ccttcgcccc gcaccacccg tacaacatgc cggatggttt
      181 caccgaaacg tttggcccgg agccgggaat gaaaaagtcg caggtgcggt atcttaactc
      241 actccatttt gccgaccagg catttggcga aattcttgcg gcgctcgaat cgaagggaat
      301 tctgcggaac tctatacttg tggtgtttgg cgatcacggc gaagctttct atgaacatcc
      361 gggtaactac aaccacccgt tcttcctgta cgaagaaaat atccatgtcc cgctgatgat
      421 ctactttgat ggggttgagg cacagcatat cgcgcgtgtc acgtcgcacg tcgatattct
      481 accgactgta ctcgaccttt taggtaaaga gaagctacgc agtccgctgc atgtcgggca
      541 gtccatgctg cgcggcgggg cgcagagcct ggcgcacctg caggcgtact ggcaggaaga
      601 atattcggga atcgtcgatg cgcagttcaa atatatccgc aaagagaccg gggcccatga
      661 gctctttgat cttttgcaag atgcaggcga gcagcagaat ctcgcgcaaa gcaggcctga
      721 gctggtcacc aggtaccagt ctttgaccgc cagggcattt gcgcagaaaa agcgctacta
      781 ccagaaacac gcgggatacg aactcacacg ctttaaccct gccagccagg ataaataggg
      841 tgttgcccgg ttgaacaggt taagcgcaaa gcttagcgcg ccttagcgga attcttctgc
      901 cccgcgagcc agatcgggcc gccctttttc tttttcgtcg accacctgat ctgcgcaaaa
      961 cgcgccgtca ggtcatcctt agacagggta acgggagtag cgggtttctg tgcctgcagc
     1021 gcctgtgtgt acttcctgta ccacgccgct tcttctggtg tgaggtattt gccaatatcg
     1081 agtagcgcca gcttctggcg cagttggttt tccagagccc gctgcagcgg cagcggctgg
     1141 ctttcggcat tacggccggt cttcagcgta cctgactgta tcgctgcctg caaacgtgca
     1201 tgcgcttcag gcggttcttc ctgccacgag gtatagagca gcacatctgc gcccgcgaga
     1261 attgccgtaa cgggacggtc gagcgtctta aaattctggc tcaccgcgtt catctccatc
     1321 gcatcggtaa agacgagacc ggcaaaaccc agttcgctgc gcagtgtccc agacagccag
     1381 cgtgacgaaa gtgttgccgg gtgatcgggg tcgatctgcg ggtacaggat atgcgcggtc
     1441 atcactgcct gagcgccgtc gcggattgct tcgcggaatg gtataagctc cagtgcgcgc
     1501 agttcatcga tctttttgtc aatgacgggc agtgcccagt ggctgtctgt ctgtgtatcg
     1561 ccgtggccgg gaaaatgttt gataaccggt aaagctccac cttcgcgcat tgcctgccgc
     1621 gcgccctgct caaacggcag cgcacaaccc agaaccgcgt cgaggttagc gccgaaagaa
     1681 cgcaggccga tcaccgggtt ctgcggattg ttgttgatgt cgacgatcgg agcgaaaaaa
     1741 acattgatgc ccagtgcacc caaatcgcgg ctcacgtggt agcccgtcgc gcggcagagt
     1801 tcacggtcgc cagttgctcc catgtccata gccgggggag tctgcagtac tccttcgcgc
     1861 acgcgccgga cataaccacc ttcctggtcg gttgaaatga ggaaaggtgg caggcccagc
     1921 tctgctgcga gtgcctgtag atcggtggtg agctttttga tctgcgcggc gtcctgcagg
     1981 ttaaaaccga agaagatgat tccgcctggt tttatcgctg cgatcgcctt gcgcgtttct
     2041 tctgttagct cggttcccgg aacggcaata tggatgggct gtcccgcaag gcggtcttca
     2101 tccagggcag caatcgcccc ggtgataatt ttttccgtga gcgctgagta tttgcggggc
     2161 agatagatcc gatcgtacag agcataggcg acggcgatac ttatcagtac gatgagcgct
     2221 gcaatcgcaa gatttctatt tttgcgcatg aaagacttcc tcttttttca gcagcgacag
     2281 catttttctg tcacggctcc atatctttgc acgcgattct ctcgccgcgc agatgagggc
     2341 aaggtcgatg agtcccacac cgcgggaata cgctttctct ttgtacgaga gattaccggc
     2401 ggtcacaaaa agatccgcgc tgcgcacttg cgaccgcgaa aaaattcgat ccagactgag
     2461 gtgtccggca ggatcacggg tgacgccgtt ggttcaggcg gcgcacagac gacgcgctga
     2521 agctgtcggc aaagtgcagg ggcgcctttt caatttctgc gttcaatcgg cgcagtttct
     2581 gaatacgcaa ccaatcgttg agggcgatta ccagcgattc ggtgatgttt tttccgccag
     2641 agaaagtgcg cacatcgtgt atgagctcgt catccatgag tgctgtgatc ttcatacgac
     2701 gaggcatacg atcaacaatc gtatagtaaa ccattttttt gggttatggg tgcgcacagc
     2761 gcccgccaga acccaaaaaa ttttaaatct caaacttgtc ggtcaccgcc aaagaggccg
     2821 tccgtagccg gcggtgacgt taagttccaa acttgtccgt caccgcgcct tcagaagcag
     2881 actgcacctg gtggtggtac ttggccagga tgccacggtt gtaacggggt tttggctgct
     2941 tccatgcttt tttgcgtttt gcgagttctt ttttgtctac gagcaggttg acctcgttct
     3001 ttttcgcatc gataataatc tcgtcaccat ttttgatgag cgcaatggga ccaccatcga
     3061 acgcttcggg tgtgatatgc cccacaacaa agccgtgggt tccccccgag aacctgccgt
     3121 cggtgatcag cgcgacgtct ttgccgagac ctttgcccat aactgccgag gtgggcgaaa
     3181 gcatctcgcg catacccggg ccgccacggg gaccttcata acggatcacg atgacgtccc
     3241 ccttcttgat cttgccattg agaattgcgg gcaggcagtc ttcttcgcgg tcgaatacct
     3301 tggccttgcc tttgaaatac aaaccttctt tgccggaaat tttggccaca gacccgcccg
     3361 gggcaaggtt accgtaacag accaccaggt ggctgtcttt cttgatcggg ttatcaaaag
     3421 accgaacaat atcctggtcg gcggggtaag gtttcacatt cttcaggttt tccgccagcg
     3481 tcttgcctgt tacggttagt acatcgccgt gcaacagttt acggtcgagc agcattttca
     3541 tcaggggcag ggtaccaccg atttcaatca ggcggctcat cgcgtatttt ccgctcggct
     3601 tgaggtctgc gagcacgggg actttttttc ccagccgtat gaagtcatcc agcccgatct
     3661 tgacgcccgc tgtatgcgcg atggcgatca ggtgcagcac ggcatttgtc gaaccaccga
     3721 ttgcgatcac cacggtgatt gcattctcaa atgccttcct ggtcagtatg tcgctggggc
     3781 gaatgccctg ctgcagcatg ttcagcacgg cttcgcctgc gcgcctgcag tcgtctttct
     3841 tctcagccga gatggcgttt tgtgcgctgc tgtttggcag gctcagcccc agcgcctcaa
     3901 tggcagaagc catggtgttc gcagtgtaca taccgccgca cgagccggct cccggaatcg
     3961 ctttttcttc gatctctttc agtcccgcgt cattcaggcg gccgttcgca tgagctccca
     4021 caccttcaaa caccgagacg atatcgaggt cacggccgtc agagtgttta cccggcatga
     4081 tggtgccgcc atagacgaat accgcgggcc ggttcaggcg cgcaatggcc atgacgcagc
     4141 ccggcatgtt cttgtcgcag ccgccgatgg tcacgaagcc gtcgaaaccc tgcgcaccga
     4201 ctacggtttc aattgagtcg gcgatcacct cgcgcgagac gagcgagtac ttcatgccct
     4261 cggtgcccat cgaaatgccg tcagagacgg tgatcgtatt gaactgcacg gctttaccgc
     4321 cggctgcgtt aacgcctttt tcggcctcgt tcgccagcag gttgatgtgc atattgcagg
     4381 gagtaaccat tgaccaggta ctggcaacgc ccacgacggg ttttttgaaa tccgcatcgc
     4441 cgaagcccac tgcgcgcagc attgcgcgcg agggtgtacg atcgacaccg tcgaccacct
     4501 gcgcggagaa tttccgctta tccgattttt ttttctgagc gctttttgcc atctggttca
     4561 atcccttgtc gccttcttca ggcaggtatt cctgcggagt acttcttttc gtgtgctgaa
     4621 atctcagcgt cgaactgcag ggtccagcca atgtcgtcga ggccctgcag cagcttttgc
     4681 cgcagcggct ctgcaaggtt gaaggcgata cttttgccgt cgaagctgat agtcttcttt
     4741 tcgaggtcga tagtcagttc ggtcttcgca tcgtttttga ctgcctggaa cagctgctca
     4801 acctctgctg cagagagcgc aatgggcaga atgccgttct ggaagcagtt gttgtaaaag
     4861 atgtcggcga acgaaggtgc gatgatcacc tggaaaccgt aatcgagcaa cgaccatggc
     4921 gcatgttcgc gcgaagagcc gcagccaaag ttatcgcgcg caagcagcac cctggcacct
     4981 ttgtgctgcg gaaaattgag cacgaagtcc ttattttctg agccgtcgtc tttgtagcgc
     5041 cagtcattaa acagaaactg gccaaatccc gtgcgctcga cgcgtttcag gaagtttttt
     5101 ggaataatct ggtccgtgtc gacgttgggc atgtcgagcg ggcaggcgat tgattttact
     5161 gtgttaaatt tttccatcgt gtatctcctt aaatctggtt gccacctgcg tcaggcagta
     5221 acctcgctac gggcatcaac gaaatgcccg tggattgccg caagcgctgc catcgccggt
     5281 gaaacgaggt aggtacggcc acctttgccc tggcgccctt cgaagttgcg gttagaggtc
     5341 gaagcgcagc gttcacccgg ctcaagtacg tcgtcgttca tcgcgaggca catcgagcag
     5401 cccggctcgc gccattcaaa gcccgcgctc gtgaaaatac ggtgcaggcc ttctttttct
     5461 gcctggtcct tcaccctatg gctaccgggc acgaccatcg cgagcttgat gttcgccgca
     5521 accttgcggt tcttgatgac cttcgctgct tcgcgcaggt cttcgatgcg cgagttggtg
     5581 cacgagccga taaatacctt gtcgagtttg atttggtcta tagctgttcc ctcggcgaag
     5641 tccatgtatt cgagtgcctt cttgatgccc acgcgttcta cttcgtcctt
//
LOCUS       DAOQJF010000215         5668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8201_length_5668_cov_5.856048, whole genome shotgun sequence.
ACCESSION   DAOQJF010000215 DAOQJF010000000
VERSION     DAOQJF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8201_length_5668_cov_5.856048"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLF85_11380"
     CDS             complement(<1..1387)
                     /locus_tag="PLF85_11380"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNE20313.1"
                     /translation="MPFHYFHSERLGPLFPVLHDEIRRERRTLPPQKTQAIIVGNLET
                     EAWITRRFLAADGILMGVSFPFLESAIGSFSERLELQLSPTSSESWFLPPAGERPRAL
                     GTAEFEILLLGILNDKANGELLHKLGYATDQLTPSQLTTLATVLADELRETILHRPTL
                     LNEVGKGKQQGKSAGEKLWRALYHSLQATERPFPAFEPGLADQIAAHQLPAGSVTETL
                     YLFGMPMLSEYHLRALAAIARHASVHLYMTDLAAHATSDNGLLATAGKKAKAFVALLR
                     DVCKSYAADFTVTAVSADAEPGKSFTVYALPGIWRGAELIGDEFHNLLMRNPDVYQDD
                     IAVSLTNPSLQYAAFERALAMRQLVAFSRERFYEVPHPLSELWQIIADIAQGGLSRPL
                     IVRYALHPLVAEKHAIEPDSIQQWLTALEKAHGYRDDYPEAQAVFGLEAALRRIERGI
                     IMRNTQDTDLPA"
     gene            complement(1387..2028)
                     /gene="aat"
                     /locus_tag="PLF85_11385"
     CDS             complement(1387..2028)
                     /gene="aat"
                     /locus_tag="PLF85_11385"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNE20314.1"
                     /translation="MTADISQLTCRYAFPSTEGLGEDMYLGAGADLEPDTLLYAYSHG
                     FFPWYEKEPILWCSPPERTILQAGELHLGRTLRRILRKNPYSLTRNAAFSEVIRHCAE
                     SRSETWITPEMERAYNSLHALGFAESWEAWSDNELVGGIYAVVLNRAAFLESTFHYAD
                     NAGKVALVSLYETLCSEGITLFDFQVPSEIAETFGARTVGRSVFENLLREAIA"
     gene            2146..3000
                     /locus_tag="PLF85_11390"
     CDS             2146..3000
                     /locus_tag="PLF85_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE20315.1"
                     /translation="MSLQIKYYGVRGSLPVSGPEFARFGGNTTCCYVQHKDTRIVIDA
                     GTGIRILGLDLMASELPKTGGKLHLLFTHTHWDHIQGFPFFIPAFLPNVELSVYGETK
                     TVPGLDGNEETWDIERVLRMQQHFMYFPVGTKYMSSIKNFHAINSESRFRIGEVEVSC
                     FALHHPNSTLAFRFDADGKSFVFSTDVEHNDDMIKRLSAFAQGADALAYDCQYTTAEY
                     EAGKIGWGHSTYESAIEICKRGNIKNLHMIHHDPLHTDNTLLSMEADAKSKFPAATMI
                     PESFECTL"
     gene            3054..4790
                     /gene="argS"
                     /locus_tag="PLF85_11395"
     CDS             3054..4790
                     /gene="argS"
                     /locus_tag="PLF85_11395"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNE20316.1"
                     /translation="MTLRNLIRLKVAAAAEKVAAENQLQLPENFAPRIDYTPELKFGH
                     YATPAAMELAKAFRLPPRAIAEKIIAVMDKEFFAKVELAGAGFINFTLNNEAMAQVAA
                     FSALGEWLSVNETELRKEKIIFEYVSANPTGPMNIVSARAASVGDSITRVLRAAGHEV
                     HREYYVNDYGNQVEKLGLSFAYRYLQKYKSVTLPEDCYQGEYIIAYMEKLVAANEIPA
                     DLALSAEKVTALDESAWLAKAAVFFAPRGVKAILADQQQDLAAFRTSFDNFFSEASLH
                     ARGAVLMACENLEKRGKIQLSEGAKVFTSTEYGDDKDRVVVRSDGRPTYLLADIAYHA
                     DKVARGYKTIIDIWGPDHHGYIARLSGALKALGFGDSADEVFRVIIAQQVNLLENGEP
                     VVMSKRLGKFQTMRDLIEKIPVDVCRWFFVQRGLSTHLDFDLTLALDQSKKNPVYYVQ
                     YAHARIHSIFRECGQPPEKTDLQALAARCAGHAEREALLYWLIRFPELLQDIARNYEI
                     QRLPEYLYQTATLFTSFYHGKGNRIKDLRGTAPEEASDLLMLCNLAAQVIESGLGLLG
                     ISAPREMQKEEE"
     gene            complement(4836..5315)
                     /locus_tag="PLF85_11400"
     CDS             complement(4836..5315)
                     /locus_tag="PLF85_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20317.1"
                     /translation="MKKVAYSALFAVAFLATYCGGGTKGLQDISQGEGSVDSLEEGFI
                     GEDKLIVKGDGVSPEGMKTALQKEAAAKEAARIDGQRKVIEICRGANIQAAATVDNFA
                     LAGSAVGKDLAGRMKGAKVKKANCKPDGDRVGCKVLMEIQKKGIKKDCELAMAELSK"
BASE COUNT         1225 a         1540 c         1590 g         1313 t
ORIGIN      
        1 cggcgggcaa gtcggtatcc tgcgtgttac gcataataat cccgcgttcg attctgcgta
       61 gcgcagcttc aaggccgaac acggcctgtg cttcgggata gtcgtcacgg tagccgtggg
      121 ccttttcgag tgctgtgagc cactgctgga tgctgtcggg ttcgatggca tgtttctctg
      181 ccacaagcgg gtgaagcgca taacgcacaa tcaacggccg gctgagacca ccctgcgcta
      241 tatctgcgat aatctgccag agttcggaca gcggatgagg cacttcatag aatcgctccc
      301 gtgagaaagc aacgagctgc cgcatcgcca aagcccgctc aaatgcggca tactgcagac
      361 tcggattagt cagcgataca gcaatgtcat cctggtagac gtccgggttg cgcatcaaca
      421 agttatggaa ttcgtcgccg attagctccg ccccgcgcca gataccgggt agggcgtaga
      481 cggtgaatga cttgccaggt tctgcatctg ccgaaactgc tgtgacggta aaatccgcag
      541 cataggattt gcagacatcg cgcagcaggg cgacgaaagc ttttgctttc ttgcccgcgg
      601 ttgccagcag gccgttatcg ctcgttgcat gtgcggcgag gtcggtcatg taaagatgta
      661 ccgaagcgtg gcgcgcgatg gccgcgaggg cacgcaggtg gtattcagaa agcatcggca
      721 tgccaaacag atacagtgtc tcggtgactg agcccgcggg caattggtgc gccgcgattt
      781 ggtcagcgag acctggctca aaggcgggaa acgggcgctc cgttgcctgc agggagtggt
      841 agagagcccg ccagagtttt tctcctgcac ttttaccctg ttgtttacct ttgccgactt
      901 cgttgagcag agtcggacgg tgcagtatgg tctcgcgcag ttcatcggcg agtactgtgg
      961 caagagttgt taactgcgat ggcgtcagct ggtcggtggc ataccccagc ttgtgcagca
     1021 gttcgccgtt ggccttgtcg ttcagaatgc ccagcaggag tatttcgaat tcggcggtac
     1081 ccagcgcccg aggccgctcg ccggcaggtg gcaaaaacca ggattcgctg ctggtcgggg
     1141 aaagttgcag ttcaagccgt tcactgaaac tcccgattgc cgattcgaga aacgggaaac
     1201 tcacacccat caggatgcca tcggccgcga gaaagcgccg cgtgatccag gcttcggttt
     1261 cgagattgcc cacgataatc gcctgcgttt tttgaggtgg cagggtgcgc cgctcgcggc
     1321 gtatttcgtc gtgcaggacg ggaaaaagcg gaccaagcct ttctgaatgg aaatagtgga
     1381 atggcatcag gcaatcgctt cgcgcagcaa attctcgaac acgcttcggc caaccgtccg
     1441 ggctccaaag gtctctgcga tttcagatgg tacctggaag tcgaacaggg taattccctc
     1501 agaacacagt gtttcataga ggctaacgag ggctaccttg ccggcattgt cagcgtagtg
     1561 aaaggttgat tcgagaaagg cggcgcggtt cagtacgact gcgtagattc ccccgacgag
     1621 ctcattgtcc gaccaggcct cccacgattc ggcaaaaccc agtgcatgca gactgttgta
     1681 cgcgcgttcc atttcgggag ttatccaggt ttcgctgcgg ctttctgcgc agtggcggat
     1741 tacctcgcta aacgccgcat tgcgcgtcag ggagtaggga ttcttacgca atatgcggcg
     1801 caaggtccgt ccgaggtgaa gctcccccgc ctgcaggatc gtgcgttcag ggggtgagca
     1861 ccagagaatg ggttcttttt cgtaccaggg aaagaaccca tggctatagg cgtagagcag
     1921 ggtatcgggc tcaagatctg ccccggcccc aaggtacatg tcttcaccca gcccctctgt
     1981 ggagggaaac gcatagcggc atgtcagttg cgaaatatcg gcagtcatgg tttcatttgc
     2041 ccggagttct gccacgcggg catttttggt ttcggcccgg tctcacttat ctgctgtgac
     2101 cgggcattca gtttataccc tgcgcagaac gatattagaa taagcgtgtc actgcaaata
     2161 aaatattatg gcgtcagggg ttctttaccc gtcagcgggc cggagtttgc ccgttttggg
     2221 ggtaacacaa cctgctgtta cgtgcagcat aaggacacgc ggatcgtcat cgatgccggc
     2281 accggcatca ggatccttgg cctcgacctg atggcgagcg aactgcccaa gacgggcggc
     2341 aaactgcacc tgctttttac ccacacgcac tgggaccaca tccaggggtt tcccttcttt
     2401 atacctgcgt ttttgcccaa tgtcgagctg agtgtctacg gtgaaaccaa gacagtcccg
     2461 ggtctcgacg gcaacgaaga gacgtgggat atcgaacgtg tgctcagaat gcagcagcat
     2521 ttcatgtatt ttcccgtggg taccaagtac atgtcatcga tcaagaactt ccatgcgata
     2581 aattctgaat cgcgtttccg catcggcgaa gtggaggtct cgtgcttcgc gctgcaccac
     2641 ccgaattcga cgctggcatt ccgtttcgac gccgatggca agagttttgt tttttccacc
     2701 gatgtggagc acaacgacga catgatcaag cggctttccg cttttgcgca gggtgccgac
     2761 gcgctcgcgt acgactgcca gtacaccacg gcagagtacg aggccggcaa gatcggctgg
     2821 ggtcattcta cttacgaaag cgccattgag atctgcaagc ggggcaacat caaaaacctg
     2881 cacatgattc accacgaccc gctgcacacc gataacaccc tgctttcgat ggaagctgac
     2941 gctaagtcta agtttccggc agcgaccatg attcccgagt cttttgagtg cacgctctga
     3001 cgccaggcaa agtcctgccg cattgagcca gcacctaaac cggagataat tccatgacat
     3061 taagaaacct cattcgcctc aaggttgccg cggctgccga aaaagttgcc gccgaaaacc
     3121 agctgcagct ccctgagaac tttgcaccgc gtatcgacta cactcccgaa ctgaaatttg
     3181 gccactatgc cacgcctgcc gcgatggaac tcgcgaaggc ttttcgcctg ccgccgcgcg
     3241 cgatcgctga aaagatcatc gcggtcatgg acaaagagtt tttcgccaaa gtggagctag
     3301 cgggcgcagg cttcatcaat tttaccctga ataacgaggc catggcacag gtagctgctt
     3361 tttcggcgct cggtgaatgg ctgtcagtaa acgaaaccga actgcggaaa gaaaaaatca
     3421 tctttgagta cgtctctgct aatcctacgg ggccgatgaa tatcgtttcg gcgcgcgcgg
     3481 cttcggtggg tgacagcatt acgcgtgtgc tgcgtgccgc ggggcacgag gtacaccgcg
     3541 aatattacgt gaacgactac ggcaaccagg tggaaaaact tggtctctcc tttgcgtacc
     3601 gttacctgca aaaatataaa tccgtcacgc tgcccgaaga ctgctaccag ggcgaatata
     3661 ttatcgccta tatggagaag ctggtcgctg caaatgaaat tcctgccgac ctcgcgctct
     3721 cggctgaaaa ggtcacagcg ctcgacgagt ctgcatggct cgctaaggcc gccgtcttct
     3781 ttgccccgcg cggagtgaag gcgattttgg ctgaccagca acaggacctg gcagctttcc
     3841 gcacttcgtt cgataacttc ttttctgaag cttcgctgca tgcccggggg gcagtgctca
     3901 tggcctgcga aaacctggaa aaacgcggca agattcagct gagcgaagga gctaaggttt
     3961 tcacgagtac ggaatacggt gacgataaag accgggttgt tgtgcgttct gatgggcggc
     4021 caacgtacct gctcgccgat atcgcctacc atgccgacaa agtagcacgc ggttacaaaa
     4081 ccatcattga tatctgggga cctgaccacc atggttacat cgcgcggttg tcgggtgcat
     4141 tgaaagcgct cggctttggt gattcagccg atgaagtctt tcgtgtgatc attgcacagc
     4201 aggtgaacct actggaaaat ggcgaacctg tagtcatgtc caagcggctg ggcaagttcc
     4261 agacgatgcg cgacctgatc gagaagatac ccgtcgacgt ctgccgctgg ttctttgtcc
     4321 agcggggcct gagtacccat ctcgattttg acctgacgct ggcgctcgac cagtcgaaga
     4381 agaacccggt ctactacgtg cagtatgcgc acgcgcgcat ccactcgatt tttcgcgagt
     4441 gcggccagcc gccggaaaag accgacctgc aggcattggc ggcccgctgt gcagggcacg
     4501 cagaacgcga agcgttgctc tactggctga tccgttttcc tgaactcctg caggatatcg
     4561 cacgcaatta tgagatacag cgcctgccgg agtatctcta ccagactgcg acgctgttca
     4621 catcattcta ccatggaaaa ggtaaccgca tcaaagacct gcgcggtacg gcacctgaag
     4681 aagccagtga tctgttgatg ttgtgtaacc tggcagccca ggtgattgaa tcagggcttg
     4741 gacttctggg catcagcgct ccgcgcgaaa tgcagaaaga agaagaataa aaaaggcggc
     4801 ttgcgccgcc ttttttattc gttatgtgcc taaacttatt tagacagttc ggccattgcg
     4861 agttcgcaat ctttcttgat gccttttttc tgaatttcca tcagaacttt gcagcccaca
     4921 cggtcaccgt ctggtttgca gttggctttc ttgacttttg cgcctttcat gcggccagcg
     4981 aggtctttac ctacggctga accagcgagg gcgaagttgt caacagttgc agctgcctgg
     5041 atgtttgcac cgcggcagat ttcgataact ttacgctggc cgtcgatacg cgcagcttct
     5101 tttgctgctg cttctttctg cagcgcagtt ttcatgcctt ctggtgatac gccgtcgcct
     5161 ttgacgatca gcttgtcttc gccgatgaag ccttcttcga gtgagtctac tgaaccttca
     5221 ccctggctga tgtcctggag cccttttgtg ccgccaccgc agtatgttgc gaggaaagcg
     5281 accgcgaaaa gtgctgaata tgctactttc ttcatgtttt ctcctgttgt ttaattaccc
     5341 ttgcttgcgc cgggtcgccc gtttgagctt tctgcacgat tgcacggatg caatcacggg
     5401 tcaagcctaa aggcgggcaa tttctcaact acggttagac gtttggtgcc gcaaaaaggg
     5461 tcgttttttt cctggctgac gggtgttttc gaggtgcctt catttaattg ccgcgctgtc
     5521 tgaacccttc gttcagacac atgcagatcg tcgatcgcga aggtacagcc gaagaggcaa
     5581 aaaaactagg cctaaaagac ggggagttcg acctgatcgt cggccacctg ggccgtaagc
     5641 cgtctttcac cgagctcgcg atgttttc
//
LOCUS       DAOQJF010000216         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8254_length_5644_cov_5.340311, whole genome shotgun sequence.
ACCESSION   DAOQJF010000216 DAOQJF010000000
VERSION     DAOQJF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8254_length_5644_cov_5.340311"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLF85_11405"
     CDS             <1..856
                     /locus_tag="PLF85_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20318.1"
                     /translation="NFLGQNKGLAVGALASNRGWQALAGFTDPHIAYSRFTTTFRYLT
                     GKVFIEDATPAGVVTESYTMMRHDFQSATGYQWLSGFFVGVLAGYRSAVMTDNAPLAP
                     SSVMNIGARLRFSNVTPEGFFQSGYETTLDLERGNVFTGEKLHVISSASAMHVRISRS
                     HFLSVLGSFQYSAYPRQLEQRLGGWQGTRTLPALLIPADRFAVAAVNYQVALFTFPWA
                     TFAALAFVDAGSAAHDRESPIYFWGPGAGVRMYLTEITVPALGVDAARDMLSQQVQIS
                     FFVGYNLQ"
     gene            853..1566
                     /locus_tag="PLF85_11410"
     CDS             853..1566
                     /locus_tag="PLF85_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20319.1"
                     /translation="MKTVLIITGPESEFGSGHAMRMHTLSLELKRHKVTVQKVVLSEG
                     EDAALPLDYQLAILDRRDTAFPASVLGSNAMRIALDNRGFGREQAHTCYNALPHPAMD
                     DNEYRTALSAVLLPVHVTSLPCRSAEAKVTLHADESSARAAADFPRVSGRLSPRQFTE
                     SMRQAESVACYFGQAMFEAIYLGKRVELYPVSEYHRQLAEDFTQRLARQQDFLSALDG
                     SGPGRLRQFVLRVLKETAR"
     gene            1682..2701
                     /locus_tag="PLF85_11415"
     CDS             1682..2701
                     /locus_tag="PLF85_11415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNE20320.1"
                     /translation="MVVSLSLTDNSAAKEKTANRIRIKAVGDIVPGTLYPEKRVPAHP
                     YDDIFAPIQQYISGADILFGNFESTLTDYPKTSKNTASKHVFAFRTPPSTAAVLKKAG
                     FHVLSTANNHSGDFGERGFNDTMENLKEAGIIGVGRKGQVAYTRAQGKRVAFIAFSYF
                     RKHNYIHDLDEMRALVKKARENADIVVISCHWGGEGEKFLHTKRETEIFFGENRGNLP
                     LFAHAAIDAGADLILGHGPHVVRALELYKNRLIVYSLGNFIGYGALSSRGKTGLTLVL
                     DIDLDNEGRFLGGKIHPMHMPEKSLPRHDPSGKTIELMRSLTEKDFPKTKLEIGESGV
                     ISISR"
     gene            complement(2732..5308)
                     /gene="clpB"
                     /locus_tag="PLF85_11420"
     CDS             complement(2732..5308)
                     /gene="clpB"
                     /locus_tag="PLF85_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNE20321.1"
                     /translation="MAAENMTLKTRTVYQAAVSRAQELGHADITPEHFMASLFTDTDG
                     LPVQVLHRMGVEPQSVTRLFETYLSKLPRAQGNFEPGQNLSNDARKFLQLAEREMSGL
                     GDQYLSLEHLLLAYTNGSFTLKKELEGLGLERRRLAEILKELRGNQKADSDNPEAKFN
                     ALQKYGKNLNELARQGKLDPVIGRDEEIRRTIQILSRRNKNNPLLIGEPGTGKTAIVE
                     GLAGRIVTGDVPEILKDKTVITLDMGSLIAGAKYRGEFEERLKAVLDEVRANQDKLIL
                     FIDEIHTVVGAGAGEGQLDAANMLKPALARGEIHLIGATTLKEYQKHLEKDAALERRF
                     QSVYVTEPSVEDAVTILRGLRDRYEVHHGIRITDAAIVSAATLSHRYISDRFLPDKAI
                     DLLDEACAKLKIERNSMPQELDDLNRRIVALTIEETALNRENDTASVERLNAVRKELG
                     ELREEFKAMKLKLDSEKQDLTQVSTIKEEIEKLKVEEAAAERKGDLNRVAELRYGKIP
                     ELQKKLEAAVTQMRGKTDGVRLLKEEVTEEDIAEIVSRWTGIPVSKMLTSEKTKLLKI
                     EEALHDRVVGQETAVEAIANAIRRNRAGIADEDKPIGSFLFLGPTGVGKTETAKALAE
                     YLFDDTKAMVRIDMSEYMEKHSVAKLIGSPPGYIGYDEGGQLTEKVRRRPYSVILFDE
                     IEKAHPDVFNLFLQILDEGRLTDAKGRLVNFRNTILIMTSNLGSEHLMNRDLNTEEQE
                     VRVLETVRGYFRPEFLNRLDSVIQFHRLGQEHLQKIAGLQIDRIVEKIRSRGIELEVG
                     DDVRKLIIENGFDEEYGARPMKRAIENLLLNPLATTLLEGKFEQGKKITAKRRLDGVD
                     FV"
BASE COUNT         1146 a         1680 c         1523 g         1295 t
ORIGIN      
        1 gaacttcctc gggcagaaca agggcctcgc ggtgggagct cttgcgtcga accggggctg
       61 gcaggcgctc gccggtttta ccgacccgca tattgcctat tcgcgtttca caacgacttt
      121 ccgttatcta accggcaaag tattcatcga agatgcgaca cccgcgggtg ttgtgactga
      181 aagttacacg atgatgcggc acgacttcca atcagcgacg ggttaccaat ggctgtccgg
      241 ttttttcgtg ggagtgcttg ccggttaccg ttcggctgtg atgactgaca atgctccgct
      301 tgctccctcg tcggtgatga acataggcgc acgcctgcgc ttctcgaacg tgacccccga
      361 agggttcttt cagtcgggtt atgaaacgac cctcgacctc gaacgcggta atgtcttcac
      421 gggcgaaaag ctgcacgtca tttcgtccgc aagtgcaatg cacgtgcgaa tttcgcgctc
      481 ccattttctg agcgtactgg gctctttcca gtattctgcc tacccgcgcc agctggagca
      541 acgccttgga ggctggcagg gcacgcgtac gttgccggcg ctgctcattc ccgccgaccg
      601 ttttgcagtt gcggcagtaa actaccaggt cgccctcttc acctttccgt gggcgacttt
      661 tgcagcgctt gcgtttgtcg acgccggcag cgccgcccat gatcgagaaa gcccgatcta
      721 cttctggggc cccggtgccg gcgtgcgcat gtatttaacg gaaatcacgg taccggcgct
      781 tggggttgac gccgcacgcg acatgctgtc tcagcaggta caaatcagct tctttgttgg
      841 gtataacctg cagtgaaaac cgtacttatc atcaccgggc ctgaaagtga atttggcagc
      901 gggcatgcaa tgcgcatgca tacgctctca cttgagctca agcgccacaa ggtaacagta
      961 cagaaagttg tactcagcga gggagaagac gcggcgcttc cgctcgatta ccagctggca
     1021 attcttgacc ggcgcgatac ggctttcccg gcatcggtac tgggcagcaa tgccatgcgc
     1081 atcgcgcttg ataaccgtgg ttttggccgc gagcaggcgc atacctgcta caacgctttg
     1141 ccccaccccg cgatggatga caacgagtac cgtacagcac tctcagcggt cctgcttccg
     1201 gttcatgtga caagcctgcc ctgccgttca gcagaggcaa aggtgacgct ccatgctgat
     1261 gaaagcagtg cgcgggccgc ggcggatttc ccccgggttt caggccggct ctcgccccgg
     1321 caatttaccg aatcgatgcg gcaagcagag agtgtcgcgt gctatttcgg ccaggcaatg
     1381 ttcgaggcga tctacctggg caaaagggtt gagctttacc cggtgagcga ataccacaga
     1441 cagctcgcag aggatttcac ccagcgcctt gcgcggcagc aagacttcct gtcagcgctc
     1501 gatggctcag gccctggccg cctgcggcag ttcgtgctgc gcgttctgaa ggagacggct
     1561 cgataggtca tcctgtccgc cggctccccg gtacctcgtg tactaaagga gatataccca
     1621 aaccatgaat tgtttagcgt ctcaattaac actccgacac ttcgcaaagg tattcttcat
     1681 cttggtagtt tcactatcac taaccgacaa ttcggcagca aaagaaaaaa cggctaaccg
     1741 catacgtatc aaggcagtgg gcgacatcgt gccaggcacg ctgtacccgg aaaagcgcgt
     1801 gcccgcgcat ccttacgatg atattttcgc cccgattcaa cagtatattt ccggcgcaga
     1861 tattttgttc ggtaacttcg agagcacact caccgattac ccgaaaactt cgaaaaatac
     1921 cgcgagcaag catgtctttg cctttcgcac cccgccctcg accgcagcgg tactgaagaa
     1981 ggcgggtttt cacgtgctca gcaccgccaa caaccattca ggcgacttcg gcgaacgcgg
     2041 cttcaatgac accatggaaa acctgaaaga ggcgggcatt atcggcgtcg gccgcaaggg
     2101 ccaggtcgcc tacacccgcg cccagggcaa acgcgtggca ttcatcgcct tcagctactt
     2161 tcgcaagcac aactatattc acgacctcga cgagatgcgc gcgctggtca aaaaagcgcg
     2221 cgaaaatgcc gacatcgtgg taatttcgtg ccactggggt ggtgaaggcg aaaaattcct
     2281 gcacacaaaa agggaaaccg agatcttctt cggcgagaac cggggcaacc tgcccctctt
     2341 tgcacacgcc gcgatcgacg caggcgctga cctgatttta ggacatggcc cccacgtcgt
     2401 gcgcgcgctc gagctctaca agaaccggct catcgtctat tcgctcggca acttcatcgg
     2461 ctacggcgca ctgtcgtcac gcggcaagac aggcctcacg ctcgtgctcg atatagatct
     2521 cgataacgag ggccgtttct taggcggtaa gattcacccg atgcacatgc ccgaaaaatc
     2581 tctaccgcgg catgacccat caggcaagac gattgaactg atgcgcagcc tgaccgagaa
     2641 ggatttcccg aagacgaagc tcgaaatcgg cgaaagcgga gtaatcagta tcagcaggta
     2701 gcagaagcaa gccccctgct accatccaac gctacacaaa atccacgccg tctaaccgcc
     2761 gtttcgccgt gatcttcttg ccctgctcga atttaccctc gagcagcgtt gtcgccagcg
     2821 ggttcagcag caggttctcg atcgcgcgct tcatcggccg tgcgccgtat tcttcgtcga
     2881 agccgttctc gataatgagc tttctcacgt cgtcgccgac ctcgagctcg ataccgcgcg
     2941 agcggatctt ctcgacaatg cggtcgatct gcagtcctgc gatcttctgc aggtgctcct
     3001 gccccaggcg gtggaactgg atcaccgaat ccagccggtt caagaactcg ggcctgaagt
     3061 agccacgcac cgtttcgagt acacgtactt cctgctcctc ggtgttaagg tcgcggttca
     3121 tcagatgctc cgaaccgagg ttcgaagtca taataagtat cgtattgcgg aaattaacca
     3181 ggcgaccttt cgcgtcggtc agcctgccct cgtccagaat ctgcaaaaac aggttgaaaa
     3241 catcggggtg cgccttttca atctcgtcga acaggatcac gctataaggc cggcggcgca
     3301 ccttttcggt cagttgcccg ccctcgtcgt agccgatgta tccgggcggc gaccctatca
     3361 gcttcgcgac cgaatgtttc tccatgtact cgctcatgtc gatgcgcacc atcgccttgg
     3421 tgtcgtcgaa caggtactcg gcaagtgcct tcgcggtttc agtcttaccc acgccggtgg
     3481 gccccaggaa caaaaagctg ccgatgggct tatcttcatc ggcgatacca gcgcggttac
     3541 gccgaattgc gtttgcaatt gcctcgaccg cagtctcttg tccgacaaca cgatcgtgca
     3601 gcgcctcttc aatcttgagg agcttggttt tttctgaagt aagcatcttc gagacgggaa
     3661 ttcccgtcca gcgcgagaca atctcggcga tgtcttcttc ggtcacttct tctttgagca
     3721 ggcgcacacc gtcggtcttg ccgcgcatct gggtcacagc cgcttcgagt ttcttctgca
     3781 gctcgggaat tttaccataa cgcagctcgg ccacgcggtt cagatcgccc ttgcgctctg
     3841 cagctgcctc ttcgaccttg agcttctcga tttcttcttt gatggtcgaa acctgcgtca
     3901 ggtcctgctt ttcgctgtcg agcttaagct tcattgcctt gaactcttca cgcagctcgc
     3961 cgagttcttt gcgcacggca ttcagccgct caacactcgc ggtgtcgttt tcgcggttaa
     4021 gcgcggtctc ttcaatggtc agcgccacga tacgccggtt gagatcgtcg agctcctgcg
     4081 gcatcgagtt tcgctcgatc ttgagcttcg cgcaggcttc gtcgagcagg tcgattgcct
     4141 tgtctggcaa aaagcggtcg ctgatatagc ggtgcgacag ggtcgctgct gacacgatcg
     4201 ccgcgtcggt gatgcgtata ccatggtgca cttcgtagcg gtcgcgcaaa ccgcgcagaa
     4261 tagtcaccgc atcttctacc gacggttcag tcacgtagac gctctggaaa cggcgttcga
     4321 gagcggcgtc tttttcgagg tgcttctggt attccttaag cgtggtagca ccaatcaggt
     4381 gtatttcacc gcgcgcgagc gcgggcttca gcatgttggc ggcgtcgagc tgtccttctc
     4441 cggcgcctgc gcccacaacc gtgtgtattt catcgataaa aagaatgagt ttgtcctggt
     4501 tggctcgcac ttcatcgagg acggccttga gccgctcttc gaattcgccg cggtatttcg
     4561 cacccgcgat cagagaaccc atatcgagcg tgataacagt cttatccttg agaatttcag
     4621 gcacgtcacc ggtgacaatc cgccccgcca gaccttcgac gatcgctgtc ttaccggtac
     4681 caggttcgcc gatgagcagc gggttattct tgttgcggcg cgagagaatc tgtatcgtac
     4741 ggcgaatctc ttcgtcgcgg ccgatcaccg ggtcgagttt accctgccgc gcgagttcgt
     4801 tgaggttctt gccgtatttc tgcaacgcgt taaacttcgc ctcggggttg tccgaatcgg
     4861 ccttctggtt accgcgcagt tctttcagaa tttctgcaag tcggcgccgc tcgagcccca
     4921 ggccttcaag ttctttcttc agcgtaaaac tgccattggt atacgccagc agcaggtgct
     4981 cgagcgacag gtactggtcg cccaacccgc tcatttcgcg ctcggccagc tgcaaaaact
     5041 tgcgcgcgtc gttcgaaaga ttctgtccgg gttcaaaatt gccctgcgcc cgggggagct
     5101 tagagagata cgtttcaaac agccgtgtaa cagattgcgg ctccacgccc atgcggtgca
     5161 ggacctgtac cggcaggcca tcggtatcgg taaacagcga cgccatgaag tgttcgggtg
     5221 tgatatccgc atgtccgagc tcctgcgccc ggctgaccgc cgcctgatac accgtcctgg
     5281 tcttaagagt catattttct gctgccataa aatcccctgt tcttctggct cgttatggaa
     5341 ttccatagcc gagccagttc ttgcttgcca gagagtgata gcactcaata gccaacagtg
     5401 ctattatagt aaagacaggg cttcccagca tttttttctt acttctacaa tcaggatagt
     5461 aatagtccgg gaaagccgga cagaagcggg ctaaaggttt tctgccagcc actgcgcgtc
     5521 gaggcgcctc ggaaaataga tgccgtctgt tcccaggtgc ggctggtact taccgcgcac
     5581 ggtgagctcc aggcaaggca gcttcgtcca ggcctgtaca ttgcggaaaa gctgccggat
     5641 ctgg
//
LOCUS       DAOQJF010000217         5606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8345_length_5606_cov_5.130967, whole genome shotgun sequence.
ACCESSION   DAOQJF010000217 DAOQJF010000000
VERSION     DAOQJF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8345_length_5606_cov_5.130967"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLF85_11425"
     CDS             complement(<1..930)
                     /locus_tag="PLF85_11425"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HNE20322.1"
                     /translation="MRHIRAKSIYPGRIRGLMAEFRRERFISEEKADINPRSEFRNFK
                     SALRAAQLQLAGEKKMMLDRRRKNPGEMAAIDEAIDVLESHILTLEDPFFLGEIKNRI
                     FGGGATAAAALGAVISMVSRQFTFDSPARGLAPAPQNRHFLKDMTDMKRRIQFFLGLQ
                     QKLRLPDGVAIVAAPRLTVSEVLALKRAGVRGIIIGETSDTAHEIVMLRAMRIPCVSV
                     ADARFFRLKKQTEVLLDADIGYVVLQPKKSARFIESNPESEAKRWSVQVRMQSGEKYN
                     LSGTMHFIGELGETNQHKKDERIGLYRTEYQISE"
     gene            1069..2082
                     /locus_tag="PLF85_11430"
     CDS             1069..2082
                     /locus_tag="PLF85_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20323.1"
                     /translation="MLRILAGILLSVLLAEAVVAASWRNNKTDHHGNATENFSSSGLK
                     LYVSSPRLRFARNEDISFQVSLVNEGAYPVTVYLHENPLKNFTIIARDQDGRSLNVKE
                     EMYFRFGDDYRDPHHTRYTGEMYPMRKLILHAGERYEKKIRLADYIEWEGFSDRVKNL
                     QISAHFYPNPVQAERYFLASAGSLNLLYDRSREVLPERDNRQDAGIAPLAVSAREIVY
                     LMLSAEYTKNWSQYFKYLSLKDVIRDYPEFLRLYAQNSTDEQVKALSEFRKYLSGRGS
                     HRLIKFRVLGGETREEGETAQVRVRARRDVEGFERDYITTYYLTKRDTLWQITGIESR
                     LAD"
     gene            2126..3154
                     /gene="fliM"
                     /locus_tag="PLF85_11435"
     CDS             2126..3154
                     /gene="fliM"
                     /locus_tag="PLF85_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNE20324.1"
                     /translation="MTDILSQDEIDQLLSAISSGDAQTEDFSATQAEQKKVKIYDFKR
                     PDKFSKDQIRTVQMMHETFARLTTTALSAKLRAMVSVHVQSVDQLTYEEFIRSIPNPT
                     TLSLINMDPLKGSAILEVDPSITFTIIDRLFGGAGEHAKLTRELTDIEMSVVEGIIVH
                     ILGNLREAWSTVIDLRPRLGTIETNPQFAQIVPPNEMVMLITLETKIGDSEGMMNLCI
                     PYITIEPIIQKLSAQYWFSSIRRGESEVNRTMIQSRLDNVQVQVSVEIGTVNLGFKDI
                     MNLKVGNIIKMPKTTVRDEFTFKVEERSKFKCRPGRIGSRLAVQIGEPVVDIPDELLI
                     GNRSEEEA"
     gene            complement(3194..3529)
                     /locus_tag="PLF85_11440"
     CDS             complement(3194..3529)
                     /locus_tag="PLF85_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HNE20325.1"
                     /translation="MKKLFLFAVLAPMFALFAEGVGGTYTVAGKNPGAGRYEGTCVIS
                     GDASTGYTFQWTIGEQQFSGTGTLDGDTMTVDWGQPDPVVYKVSADGAELKGKWGPNG
                     KGREKLTRQ"
     gene            3682..5055
                     /gene="miaB"
                     /locus_tag="PLF85_11445"
     CDS             3682..5055
                     /gene="miaB"
                     /locus_tag="PLF85_11445"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNE20326.1"
                     /translation="MAVAPQTNTEQSSAEKKRFYIETYGCQMNEYDSLLAAKILEENA
                     TRTNEASEADIILVNTCAIRENAHQKVYNRLRELSRFHKRGAKVGVLGCMAQNLREDL
                     LYENLPVDFIMGPDALRNLRSVTGAAGADQKSFLTLSRTETYDDITPSFEHHMRDREN
                     QTTAFVTIQRGCDNFCAFCVVPYTRGRERSRPLLSIVDEVKALVENGVKSVVLLGQNV
                     NSYHHEEARFVHLIEALLRQTEAERIYFTSPHPKDFPLELIDLMAREERFANQVHMPL
                     QAGANTTLKRMKRNYTREQFLEIVGNFRSRVPDVAISTDVIVGFPGETEADFRETLEV
                     MELADFDSAFMFAYSERKGTIAQRLYPDDIPEEVKKARLAELIEKQLARSLRNNRRYI
                     GQNVTVMAEQPSRRNPNEWVGRMRNGRRVVFAPASTVDASFAGSLVTLAIHDASSQVL
                     HGRSVNG"
     gene            5048..>5606
                     /locus_tag="PLF85_11450"
     CDS             5048..>5606
                     /locus_tag="PLF85_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE20327.1"
                     /translation="MAEEVLRFENVWYGGDYARDHVDNVSFSLNRGEGMLISGPEGSG
                     KSLILDLVMAKRLPKHGKIWHDGVILDLKKETEVENLRFSIGYVTQTAGLINNLSVLE
                     NIILPLRYHTEMKDDELFAAADIWIDRYELGHRKNARPVALSASEMMRTAIIRALIVE
                     PRILLLDSIVDALCPLASRRMLELLF"
BASE COUNT         1269 a         1559 c         1536 g         1242 t
ORIGIN      
        1 ctcactaatc tggtactcgg tacggtagag gccgattcgc tcatctttct tgtgctggtt
       61 tgtttcgccg agctcgccga taaagtgcat ggtgcccgaa agattatatt tctcgcccga
      121 ctgcatgcgc acctgcacag accagcgttt ggcctcgctc tcggggttac tttcgataaa
      181 ccgcgcagac tttttgggtt gcagtacaac atagcctatg tcggcgtcga gcagtacctc
      241 ggtctgcttt ttcagccgga agaaacgtgc atctgccacc gacacgcagg gtattcgcat
      301 tgccctgagc atcacaatct cgtgtgccgt atcactggtc tcgccgatga taatgccgcg
      361 cacccccgcg cgcttgagcg cgaggacctc gctcaccgtc aggcgcggcg ctgctacgat
      421 cgcgacaccg tcaggcaggc gaagtttctg ctgcagcccg aggaaaaact ggatgcggcg
      481 tttcatgtcc gtcatgtctt tgaggaagtg ccggttctgc ggtgcaggtg ccaggccgcg
      541 ggccggtgaa tcaaaggtaa actgccggga aaccatgctg atcacggcac cgagggccgc
      601 cgctgcggtc gctccgccgc caaaaatacg gttcttgatt tcgcccaaaa agaatgggtc
      661 ttcgagtgtc aaaatatgcg attcaagcac atcgatcgct tcgtcgatcg ctgccatctc
      721 accggggttc ttgcggcgcc ggtcgagcat catttttttc tcgccggcca gctgcagctg
      781 cgccgcgcgc agcgcgctct taaaattcct gaactcgctg cgggggttga tgtcggcctt
      841 ttcttcggag ataaagcgtt cacgcctgaa ctccgccatc aggccgcgaa ttctgccggg
      901 gtagatactt ttcgccctga tatgccgcat gccggttcag gcagcatatc ctacattgcg
      961 cctgttgcag caagaaaaag caaaggtcag ccaggcgggt accctcagcc caaaaagcac
     1021 tcccaaaaaa ccctcaacga agagacatat tccgtccgat actggtatat gctccggatt
     1081 cttgccggta tccttttgtc tgtcctgctg gccgaggcgg ttgtggctgc gtcatggcgc
     1141 aataacaaga ccgaccacca tggcaatgcc actgagaact tcagctcgag tggcctgaag
     1201 ctctacgtct cttctccccg cctgcgtttt gcgcgtaacg aagatatcag cttccaggta
     1261 agcctggtga acgagggagc ttaccctgtg acggtttacc tccacgaaaa cccgctgaag
     1321 aacttcacga ttattgcccg cgatcaggac gggcgttcgc tgaatgtgaa agaagagatg
     1381 tacttccgct ttggcgatga ttaccgtgac ccgcaccaca cccgttatac cggcgaaatg
     1441 taccccatgc gcaagctgat actgcatgcc ggcgagcgtt atgagaaaaa gatccgcctg
     1501 gccgactata tcgaatggga aggattttct gaccgggtca agaatctgca gattagcgcg
     1561 catttttacc cgaaccccgt gcaggctgaa cgctacttcc tggcgtcggc cggcagtctg
     1621 aaccttctct atgaccgcag ccgcgaagtg ctgcccgaac gtgacaaccg ccaggatgcg
     1681 ggcattgccc cgctggcagt cagcgcgcgg gagattgtgt acctgatgct ttcggctgag
     1741 tacaccaaaa attggtcgca gtatttcaaa tacctctctc tcaaggacgt tatccgcgac
     1801 tacccggagt tcctgcggtt gtatgcgcag aactcaacag acgaacaggt gaaggcgctg
     1861 tcggagtttc gcaagtacct gagcggccgc ggttcgcacc gcctgatcaa gttccgcgtg
     1921 ctcggcggcg agacgcgcga agagggtgaa accgcacagg tccgcgtccg cgcgagacgc
     1981 gatgtggaag gttttgaaag ggattatatc acaacatact atttgacaaa gcgcgatacg
     2041 ctttggcaga ttacgggcat agaatcgagg ctggcggact gattttgacg actggagaaa
     2101 tcaagcgcga ctgcaaggat aacacatgac tgacattctt tcacaggacg aaatagacca
     2161 gctactctcc gcgatctcga gcggcgatgc ccagactgaa gatttttcgg cgacgcaggc
     2221 tgagcagaaa aaagtcaaaa tctacgactt caaacgcccg gacaagtttt cgaaagacca
     2281 gatccgtacg gtacagatga tgcacgaaac cttcgcgcgt ctgacaacca ccgctctttc
     2341 agctaagcta cgtgcgatgg tctctgtaca cgtgcaatcg gtagaccagc tcacgtacga
     2401 agaatttatc cgttcgattc ccaacccgac gacgctgtcg ctcatcaaca tggacccgtt
     2461 gaaagggtca gcgattcttg aagtcgaccc gtcgattacg tttaccatca tcgaccgcct
     2521 gttcggtgga gcgggggagc acgccaagct cacgcgcgaa ctcaccgata tcgaaatgtc
     2581 ggtagtggaa ggtattatcg ttcatattct ggggaacctg cgcgaagcct ggtcgacggt
     2641 gatcgatctc aggccgcgtc ttggcaccat cgagacaaac ccacagtttg cgcagattgt
     2701 tccgccgaac gagatggtca tgctgattac gctcgaaacc aaaatcggcg attcagaggg
     2761 aatgatgaac ctctgtattc cctacatcac gattgagcct atcatccaga aactctcggc
     2821 gcagtactgg ttctcttcga ttcgccgggg tgaaagcgaa gtgaaccgta cgatgatcca
     2881 gagccggctc gataacgttc aggttcaggt atcggttgag atcggtacgg tgaaccttgg
     2941 tttcaaggac ataatgaacc tcaaggtggg caacatcatc aagatgccga aaaccaccgt
     3001 gcgcgatgaa tttaccttca aggtcgaaga acgctccaag ttcaagtgcc gcccgggtcg
     3061 tataggctcg cgccttgccg tgcagatcgg cgaaccggtg gtggatatac cggacgaact
     3121 gctgattggc aaccgcagcg aagaagaagc ctgagaaaaa accagaggcc gttagccgcc
     3181 tctggttgaa cgattattgc cgcgtcagtt tttcgcgacc cttgccgttg ggcccccatt
     3241 tgcctttgag ctccgcaccg tctgcagata ccttgtagac taccgggtca ggttggcccc
     3301 agtcgaccgt catcgtgtcg ccatccagcg taccggtacc ggagaattgc tgctcgccga
     3361 tcgtccactg gaaagtgtaa cccgtggaag catcgccgct gatgacacag gtgccttcgt
     3421 agcgtccggc accgggattt tttccggcca cggtataggt cccgccgaca ccttcggcaa
     3481 atagcgcaaa catgggcgcc agaaccgcaa aaagaaaaag ttttttcata tatttcctcc
     3541 gttgttggtc tcgcgccacc ccggtggtta gagaccagcc cggcgtcctg aaccggggca
     3601 cgggtgccgt caataccatt tagcatggct actgaaaaaa gagttttcgg gctgtttctg
     3661 aacagggtat tttcaagccc aatggctgtc gcaccacaga cgaataccga gcagtcgagc
     3721 gcagaaaaga agcgtttcta tattgagacc tacggctgcc agatgaacga gtatgactcg
     3781 ctattggcgg caaaaattct cgaagagaat gccacgcgca ccaatgaagc gtccgaggct
     3841 gacatcatac tggtcaacac ctgcgccatc agggaaaacg cgcaccagaa ggtttataat
     3901 cgtctgcgcg agctcagtcg ctttcacaag cgcggcgcca aagtgggagt tctgggttgt
     3961 atggcgcaga acctgcgcga agacctgctc tatgagaatc ttccggtcga ttttatcatg
     4021 gggcccgatg cactgcgcaa cctgcgttcg gtcaccggcg ctgcaggggc tgaccagaag
     4081 agcttcctga ctctctcgcg gactgaaacc tatgacgata tcactccgtc attcgagcac
     4141 catatgcgcg accgggaaaa ccagaccacc gcgtttgtca ctatacagcg gggttgcgat
     4201 aacttctgcg ccttctgcgt tgtgccgtat acccgtgggc gcgaacgttc gcgcccgctc
     4261 ttgagtattg tcgatgaggt caaggccctg gtggagaacg gggtcaaatc tgtcgtgctg
     4321 cttggccaga acgtcaattc gtatcaccat gaagaggcgc ggttcgttca cctgattgag
     4381 gcgctgctac ggcagacaga ggcagagcgc atctatttta cttcgccgca cccgaaggat
     4441 tttcctctcg agctgatcga cctgatggca cgagaagagc gcttcgcgaa ccaggtgcat
     4501 atgcctctgc aggcaggcgc caacacaaca ctgaaacgca tgaaacgcaa ctatacgcgc
     4561 gagcagtttc tcgaaatcgt cggtaacttt cgttcgcgcg tgccggatgt tgcgatttcg
     4621 accgatgtca tcgttggttt tcccggcgag actgaagcag actttcgtga aacgctcgag
     4681 gtgatggagc ttgccgactt tgactctgcc ttcatgtttg cctattctga gcgcaaagga
     4741 acgatcgcgc aacgcctgta tcccgatgat attcctgagg aagtcaagaa ggccaggctg
     4801 gctgaactga ttgaaaagca gctggcgcgt tcgctgcgca acaaccggcg ctacatcggc
     4861 cagaacgtca ctgttatggc cgagcagccc tcgcgccgca accccaacga gtgggtaggg
     4921 cgcatgcgca atggacgcag ggttgtcttt gctccggcga gcacggtcga tgcttctttc
     4981 gctggtagcc tggttacgct ggcgatacac gatgcatcga gccaggtttt gcacgggcgc
     5041 tcagtaaatg gctgaagaag tccttcgctt tgagaatgtc tggtacggcg gcgactacgc
     5101 ccgcgaccat gtcgataatg tcagtttttc cctcaaccga ggcgagggca tgcttatcag
     5161 cggccccgaa ggtagcggca aaagtcttat tctcgacctt gttatggcca aaaggctgcc
     5221 gaagcacggt aagatctggc acgacggagt tattctcgat ctgaaaaaag aaacagaggt
     5281 ggaaaacctc cgcttctcca taggttatgt aacacagacg gcagggctta ttaataacct
     5341 ttcggtgctg gaaaacatca tcctgccact gcgttaccat acggaaatga aggacgatga
     5401 gctttttgct gcagcggata tctggattga ccgctatgag ctggggcacc gtaaaaatgc
     5461 ccgccctgtg gccctttccg cttcagaaat gatgcgtacc gctattatac gggcacttat
     5521 cgttgagccg cgcatcctgc tgcttgactc gattgttgat gccctgtgcc cgctggcttc
     5581 gcgccgcatg ctcgagcttc tgtttg
//
LOCUS       DAOQJF010000218         5542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8482_length_5542_cov_3.775287, whole genome shotgun sequence.
ACCESSION   DAOQJF010000218 DAOQJF010000000
VERSION     DAOQJF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8482_length_5542_cov_3.775287"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLF85_11455"
     CDS             <1..924
                     /locus_tag="PLF85_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNE20328.1"
                     /translation="ATIGIGHRLWVNWGHYHRYSPQLSVANGASSMVDDNVFLLVQRG
                     WQVKNTITNGIFYDNRDNIFNTTRGARVEFSHDFVGNILGGQDHFMRYYPQVDFFWWM
                     MDYTFFNLIRKNVLRRWRVVQEIRGSATFTQPTKPFWGTQDIATNPYVEIQDRLYLGG
                     YESLRGWTLFDAFYGDTPAGQTWRNGGSHRILFGSEIRVPVEPSLFWLVLFFDAGALY
                     QNMAEYSFSSTTPAATVNAYSQAQLSQQTFALSYFRYSWGFGFRLQIPILPLRIYLAK
                     RLLWDDNLGWFRDHPNQSGFEFVFGIGDRRF"
     gene            929..1804
                     /gene="serB"
                     /locus_tag="PLF85_11460"
     CDS             929..1804
                     /gene="serB"
                     /locus_tag="PLF85_11460"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNE20329.1"
                     /translation="MLGTGEVVLIAREAANVAALLRELATLGITTETTPQAAEHLQAA
                     AKYVLPLNAIVDPAGLRQLMLQHQASLLAWALRPATLRPVGLLVFDTDSTFINEEVID
                     EIAGYAGYKAHVAAITERAMRGELDFNTALAERVHLLKGLPEHTLDAVLRTKITLTHG
                     AANLIAAAKSRGVHTYLLSGGFTFFTGALSQSLGMTGHFANELEIVDGHLTGRTLGPI
                     VNRQRKAELLRELAAKHAIPLTNTVAAGDGANDMDMLSLSGMGIAFCAKPALVEASAS
                     AIFVRDLRLVQELIF"
     gene            1806..3587
                     /locus_tag="PLF85_11465"
     CDS             1806..3587
                     /locus_tag="PLF85_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNE20330.1"
                     /translation="MADLRHLLTGATADFLKKHHALGSFSADKLWDKLTEDERAALAA
                     GVESGTWRLNGRLLHSERDPKREADRAAQALAEGNAMVAVSCGAGWLLDVLPPGIRSL
                     LLIEPSAFVAAAFLLARRYEIFAGTITLFADDLSRSEALEEVLPWLQGKNLKKTTIYY
                     HAASLAAHKPLYTRAYERVVALFEKRSVNQATIVKFQQLWNKNIFLNQRVIWSSATLN
                     DLLALAPPSAIVLAGAGPSLSSSFAELREHRERFLLFAADTALVPLNKAGIYPDVVLA
                     ADPQWLNHHFAESPDAARSRWLMDPVVCPAIPHRLQQLGAKAAFWNNVFAADGIFRSS
                     DRGDVAHGGSVSTNAFDIAVRWLSARQGDTGRLILVGQDLSFSDRQAHCRGAVLEAAV
                     FNRANRLYTMEQHNLRQMKAMPVLWQKGIRQPQVRTNGKLRIFHDWFEARAADTDKSR
                     VRLINATHDGAYLRGYEHLSLSDALAGLGKESATCAWQIANTGADMRTRAEKLITGLG
                     DVYRITKENAALSRQKKPEPQTIQQLNANDAKLKATGIAREIAGLNAQALILKITEQG
                     EEPDAAQFYSTLSRAAREVRHWARRML"
     gene            3809..5035
                     /locus_tag="PLF85_11470"
     CDS             3809..5035
                     /locus_tag="PLF85_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNE20331.1"
                     /translation="MITVAGHAAAKKTVAVVNFSNSSASRSLDYLRTALPESISGTLS
                     SSREITVLERNELKQVLKEIELEQSGALDSSQVARAGKLMRADVLLVGSFTGDAERIN
                     VTLKAVDVATGVVIEGRSVSAPLATVLDEAGKASLAMASAIAGGKSGRLTVTSSPEGA
                     ELLVDGIVVGTTPVVEYRLSAGSHSIFVRKPGYESAERTTVIRAGETERLVETLAPAR
                     EAIQVFVGAGYARMFPLNSTLQQGNLFMGQLGITFGRWTADFSYGLNSGWDHSYSYSS
                     PFGALSQSREYLLNQYLLGIAVEPFPINSYISPYVGVFGGYTRVSDYRLKGSDETKEK
                     LASFDLLQLGIKAGVEFFPKLRVSLFLEARYNAFTKSVTRGTYVSQGILGEPLSVSSD
                     LSLTNISLGGGARLNF"
     gene            5046..5522
                     /locus_tag="PLF85_11475"
     CDS             5046..5522
                     /locus_tag="PLF85_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20332.1"
                     /translation="MKQLTQFLAAAAIAGALSGCIKTDDDQPKTPIFAGTEEIGLILP
                     VNTGNLGASGSFSYTVPSEVKYVVFGLFNAEIITSGKTITNPQAFIAGSRDGLADFVR
                     GSQARSALHSYNQATKDFNAAATGAVDNTYYWAVWGYDQFGNLTHSSPQRRVNLVP"
BASE COUNT         1084 a         1698 c         1632 g         1128 t
ORIGIN      
        1 gcaacgatcg gtatcggcca ccgcctctgg gttaactggg gtcactacca ccgctattcg
       61 ccgcagctct ctgtcgcgaa cggcgcttcg agtatggtgg acgacaacgt gttcctgctg
      121 gtgcagcgcg gctggcaggt gaagaatacg atcaccaacg gtatcttcta cgacaaccgc
      181 gacaacatct tcaacaccac gcgcggtgca cgcgtagagt tctcacacga ctttgtcggt
      241 aacattctcg gcggccagga ccacttcatg cgctactacc cgcaggtgga tttcttctgg
      301 tggatgatgg actacacgtt cttcaacctg atacgcaaga acgttctgcg gcgctggcgc
      361 gtggtgcagg aaattcgcgg ttcggcgacc ttcacgcagc caacgaaacc tttctggggc
      421 acgcaggata tcgcgaccaa cccgtacgtc gagatacagg atcgcctgta cctcggtggt
      481 tacgaaagtt tgcgcggctg gacgctcttt gatgccttct acggcgacac acccgcaggg
      541 cagacctggc gtaacggtgg ttcgcaccgt atccttttcg gcagcgagat tcgcgtaccg
      601 gtcgaacctt cgctgttctg gcttgtactt ttcttcgatg ccggcgcact ctaccagaat
      661 atggccgagt acagctttag ctccaccacg ccggcggcga ccgtcaatgc ctacagccag
      721 gcgcagcttt cacagcagac cttcgcgctc agctattttc gctacagctg gggtttcggt
      781 ttccgcctgc agattccgat tctgccgctg cgtatctacc tcgcgaagcg cctcttgtgg
      841 gacgacaacc tgggttggtt tcgcgaccac cccaaccaga gcggtttcga gttcgtcttt
      901 ggtatcggcg accgccgctt ctgacagcat gctcggcacc ggcgaagttg tcctgattgc
      961 ccgcgaagct gccaacgttg cagcgttatt gcgcgagctg gctaccctcg ggattaccac
     1021 agaaactaca ccgcaggccg cagaacacct gcaggccgcg gcaaagtatg tgctgccgct
     1081 caacgccatc gtcgaccccg ctgggctgcg gcagctgatg ctgcagcacc aggcgtcact
     1141 gctcgcctgg gcactgcgcc ccgcaacgct caggcccgtc ggccttttgg tattcgatac
     1201 agattctact tttattaacg aagaagtgat cgatgaaatt gccggttatg ccggttacaa
     1261 ggcgcacgtg gcggcgatta ccgaacgcgc aatgcgcggc gaactcgact tcaacacggc
     1321 gctcgccgaa cgcgtgcacc tgctcaaggg tttgcccgag cacacgctcg acgccgtgct
     1381 gcgcacgaag attacgctca cccacggcgc ggcaaacctg attgccgcgg cgaagtcgcg
     1441 cggtgtgcac acctacctgt tatcgggcgg atttactttt tttaccggcg cactttcgca
     1501 gtcgctcggc atgaccgggc attttgccaa tgagctcgaa attgttgacg gccacctcac
     1561 tggccgtaca ctggggccca ttgtgaaccg acagcgtaag gccgaactgc tgcgtgaact
     1621 cgcagccaaa catgcaatcc ccctcacaaa caccgtcgcg gctggcgatg gcgcgaacga
     1681 tatggatatg ctatcgcttt cgggcatggg tattgccttc tgcgccaaac ccgcgctcgt
     1741 cgaagcgagc gcttcggcga tttttgtgcg cgacctgcgc ctggtacaag aactgatctt
     1801 ctgatatggc cgacctgcgc cacctgctca ctggggcaac ggccgatttc ttaaaaaaac
     1861 accacgcact aggcagcttc agcgccgata agctgtggga taagcttacc gaagacgaac
     1921 gggccgcact ggccgcaggt gtcgaaagcg gtacgtggcg gcttaacggc cgcctgctgc
     1981 attcagagcg cgacccgaag cgcgaagccg accgcgcggc acaggcgctc gccgagggta
     2041 atgcaatggt ggcggtatcg tgcggcgcgg gctggttgct cgatgtactg ccaccgggca
     2101 tacgctcgct gctgctgatt gaaccctcgg cttttgtcgc cgcagccttt ctgctcgcgc
     2161 ggcgttacga aatttttgcc ggtacgatta cgctcttcgc cgacgacctg tcgcgttctg
     2221 aagcgctcga agaagtgctg ccgtggctgc agggcaagaa cctgaaaaaa acgacgatct
     2281 actaccacgc ggcttcactg gccgctcaca agccactcta tacccgcgcg tacgagcgcg
     2341 tcgtggcgct gtttgaaaag cgcagcgtca accaggcgac gatcgtgaag ttccagcagc
     2401 tgtggaacaa gaatatcttt ttgaaccagc gcgtgatctg gtcttcggcg acgctgaacg
     2461 acctgctggc gctcgcgccg ccctcggcaa ttgtgctcgc aggggcaggg ccttcgctgt
     2521 cgtcttcatt cgctgaactc agagaacacc gcgagcgctt tctgctcttc gccgccgaca
     2581 cggcgctcgt gccgctcaac aaggcaggta tctatcccga cgtggtgctc gctgccgacc
     2641 cgcagtggct gaaccaccac ttcgccgaat cacccgacgc ggcgcgttcg cgctggctga
     2701 tggacccggt cgtctgcccg gcgattccgc accggctgca gcagctgggt gctaaggccg
     2761 cgttctggaa taacgtcttt gccgccgacg gcatttttcg cagcagcgac cgcggcgatg
     2821 tcgcgcacgg tggttcggtt tcgaccaatg cgttcgacat cgcggtgcgc tggctttcgg
     2881 cgcggcaggg tgatacgggc cggctcattc ttgtcgggca ggatctgtct ttcagcgacc
     2941 ggcaggcgca ctgccgggga gctgtactcg aggctgcggt cttcaaccgc gcgaaccgtc
     3001 tttatactat ggagcagcac aacctgcggc agatgaaggc aatgcccgtg ctgtggcaga
     3061 agggcattcg ccagccgcag gtgcgcacca acggcaagct gcgcatcttc cacgactggt
     3121 tcgaggcgcg cgcggccgac accgacaaga gccgcgtgcg gctaatcaac gcaacgcacg
     3181 atggcgccta cctgcgcggt tacgaacatc tttcgctgtc tgatgcactc gcaggcctgg
     3241 gcaaagagag cgcgacgtgc gcctggcaga tcgcaaatac cggggccgat atgcgcacgc
     3301 gcgcagagaa gctcattacc ggcctgggcg atgtgtaccg cattaccaaa gaaaatgccg
     3361 cgctctcacg gcagaagaaa cccgagccgc agacaatcca acagctgaat gcgaatgatg
     3421 ccaagctgaa ggcaacgggc attgcacgcg agattgcggg cctcaatgcg caggcattga
     3481 ttctcaagat caccgagcag ggcgaagaac ccgatgcggc acagttctac agcacgctct
     3541 cgcgcgcggc gcgagaggtg cggcactggg cccggcgcat gctgtgaaat cggcttgacc
     3601 cccgccgtct cggcgacacc gtctgcctac catgcgtttg cctccattag gttccggaaa
     3661 gaattccgca tcgtctgcag acgccgcgat ttcgtgccga aatcgctcgc aaaacggcca
     3721 taaatggcct gtctgcagcc tgatgcggct tccggaacca gcgtgcggct cctttgcaag
     3781 gagccttgtt gcctgccttg cggcactgtt gattaccgtc gcaggccacg ctgccgccaa
     3841 gaagaccgtt gccgtcgtca acttcagcaa tagctccgcc tcgcgcagcc ttgactacct
     3901 caggactgca ttgcccgaat cgatcagcgg cacgctgagt tcgtcgcgcg aaattaccgt
     3961 gctcgagcgc aatgagctca agcaggtact caaagaaatt gagctggagc agagcggggc
     4021 gctcgactct tcgcaggttg cgcgcgcggg taagctgatg cgtgccgacg tgctgctggt
     4081 gggtagcttt accggcgacg ccgaacgcat caacgtcacg ctgaaggcag tcgatgtcgc
     4141 gacgggtgtc gtgatcgaag ggcgcagcgt ttctgcgccg cttgcgaccg tgctcgacga
     4201 agcgggcaag gcatcgctcg cgatggcgtc ggccattgcc ggtggcaaaa gtggccggct
     4261 gacggtgact tcaagccccg agggcgccga gctgctcgtc gacggtattg tcgtcggtac
     4321 gacccccgtg gtcgaatacc ggctgagcgc gggcagccac agcattttcg tgcgcaaacc
     4381 cggttatgaa tctgccgagc gcaccactgt cattcgcgca ggcgaaaccg aacgcctggt
     4441 cgaaaccctc gcccccgcgc gtgaagcgat acaggtcttt gtcggcgcgg gttatgcccg
     4501 catgttcccg ctcaattcaa cattgcagca gggcaacctg ttcatggggc agctgggcat
     4561 cacgttcggc cgctggactg ccgacttcag ttatggcctc aattcgggct gggaccattc
     4621 gtattcgtac tcgagcccgt tcggcgcgct ctcgcagtcg cgcgagtacc tgctcaacca
     4681 gtacctgctc ggcatcgccg ttgaaccttt cccgatcaat tcgtatattt cgccctacgt
     4741 cggcgtcttc ggcggctaca cgcgcgtcag cgactaccgc ctgaagggca gcgacgaaac
     4801 caaagaaaaa ctcgcgtcgt tcgacctgct gcagctgggc atcaaggcgg gcgtggagtt
     4861 ttttcccaaa ctcagggtga gcctcttctt agaagcccgc tacaatgcgt tcacgaaatc
     4921 ggtcacgcgc ggtacctatg tcagccaggg tattctgggt gaaccgcttt cggtatcgag
     4981 cgacctgagc ctgaccaaca tttcactcgg cggcggcgcc cgcctgaact tctgagggag
     5041 cacctatgaa acaactgaca caatttctcg cagcagcagc catcgccggc gccctctcgg
     5101 ggtgtatcaa gacagatgat gaccagccga agacaccgat cttcgcgggg acggaggaga
     5161 ttgggttgat attgccagta aatactggca acttgggcgc gagcggcagt ttcagctaca
     5221 ccgtgccaag cgaagtcaaa tatgtggttt ttggattgtt caatgcagag ataatcacct
     5281 cgggtaagac aatcactaac ccgcaggcat ttattgccgg tagtcgtgat ggcctggctg
     5341 actttgtacg cggctcacag gcgcgctctg ctttgcactc gtacaatcag gcgaccaagg
     5401 atttcaacgc tgcggctaca ggtgcagttg ataatacata ctattgggcg gtctggggct
     5461 atgaccagtt tggtaatctg actcactcgt cgccgcagcg gcgggtgaat ttagttccgt
     5521 agttgtattt ggggcgggta ac
//
LOCUS       DAOQJF010000219         5534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8503_length_5534_cov_4.509034, whole genome shotgun sequence.
ACCESSION   DAOQJF010000219 DAOQJF010000000
VERSION     DAOQJF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8503_length_5534_cov_4.509034"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..534)
                     /locus_tag="PLF85_11480"
     CDS             complement(37..534)
                     /locus_tag="PLF85_11480"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HNE20333.1"
                     /translation="MPANFKLKQSFSAHVDEVLAARERRFDDVSKQESLKKQELLDRK
                     HEGDLIVSRRAFSLADKIPEALKAMIPAGFLNLVETAKFDTKNKINRFEVVYEQNPDR
                     LRISGVTHYVHESDTSSRREYDITVKVNMALVGGMLENQIASSFRKGIEKDYEVIQEL
                     LAQGK"
     gene            667..1695
                     /locus_tag="PLF85_11485"
     CDS             667..1695
                     /locus_tag="PLF85_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE20334.1"
                     /translation="MAGSERILILEDDLAFARLMGEVLKDLGQTRVTSYPERLPQLIA
                     EFAPTLLIVDYNLNHPTMNGIKATRLVKQNPETALVPVLLLSGEDDLQVIEEAFKSGI
                     EDYVLKPVIPRFLIAKIENILFQTRRKLNAQALSGLPGNAAIETEFYLRLQKKKPFSA
                     AYTDLDHFKPFNDEKGVKKGDDAIQCVAKILYGLRANSSREQLFVGHIGGDDFLLFGS
                     KTAVAAAARRLRRDFRRETRVFFTAAELRAGGYRGTDRQGSYRSFPLLDISTAIIDRI
                     TADTVPDFSRLTELAARAKKAAKNSTTHVAAFDAGELKLPGDAKTLFVQEPGSAKKNT
                     ATARRAVK"
     gene            1744..2052
                     /locus_tag="PLF85_11490"
     CDS             1744..2052
                     /locus_tag="PLF85_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20335.1"
                     /translation="MEFAKYLIDFLVYLALAAAISWFFFFFKRLDLFGGFVGGAVVAL
                     IGSILGAFLLQGLLSDIIKFLQNGLNISNVNIIAAVLGGTVSVMLLSKINNGRQRKDY
                     "
     gene            2115..2525
                     /locus_tag="PLF85_11495"
     CDS             2115..2525
                     /locus_tag="PLF85_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20336.1"
                     /translation="MSDLAIKITESEYTPSGDGWAEARRRRLVTRYLEARGFQVRAVQ
                     RPHDDGRPASKYRQADLVAQRGAVTPEGRRARATLSLLLPEKLPVSSDFVKRQKQCDS
                     VLLSVLEKAAEFSQVELVSSPVCFGLTAQGEAQE"
     gene            2529..3467
                     /gene="ispH"
                     /locus_tag="PLF85_11500"
                     /gene_synonym="lytB"
     CDS             2529..3467
                     /gene="ispH"
                     /locus_tag="PLF85_11500"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNE20337.1"
                     /translation="MKEIIVAAPRGFCAGVDRAISIVEKALEVYGTPLYVHHEIVHNR
                     HVVEGLRAKGVIFVDTIGEIPGNQTVIFSAHGVSPAVVSEAKQKNLRILDATCPLVTK
                     VHIEARRYAREGYRIILIGHKDHIEARGTHGEAPDQMTIVETAEDIDKLQIEPGAKVA
                     YLTQTTLSVRDTRGLIELLKARFPQIEGPSSSDICYATTNRQEAVSAMAPETDVVFVI
                     GSRNSSNSNRLKELAESEGSRAYLIDSAADILPEHLAGVVKGIGITAGASAPEILVQQ
                     VTERLQKEFGFASVRELRIKEEDVIFKLPKELAAHG"
     gene            3467..5317
                     /locus_tag="PLF85_11505"
     CDS             3467..5317
                     /locus_tag="PLF85_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20338.1"
                     /translation="MRSFLVLILLPLPLAAQTYDEVKNLTKAGIAAYHQKRFAEAKDS
                     LSAALDIDSTWVPTVLHHGLAYADWLTAQIHIRNTRKLIVFEKYERADRELDEAEALY
                     PEHPEIPPLRDLIRKKRSDNTKVILEKLPDKKRKEYEESMQKAQVAMDAGKYPEAMHH
                     YSTALKVAPDSLDAKMGYAEAERMLKQQGSAQKLTVLFRQAEKYEKERRFAQAVSVYD
                     QILRIEPQNRQAIERRAALIDFMQQQLGKNERRNLAKEYLQSGNESFRKAEYDQAIDQ
                     YRIGQALDPKFADWDALIKKAMAARRAQEENLFAEKLKELERRYQSAMVQLLLENYPA
                     AIEDLEAVIAIAQQFKQAETQKQAEALLQKAKESMLRQDEEFITRDSPYYAFVQSLTT
                     LGLTSYRQRDCQATLKHFGAITEVFPKNRLSNQHILSCTIILNPERKDGIVRDLIEAI
                     YRLKDTNAYEARRLFEILKFIDPQNPAIAQLEKELAEKTTLLKKAVQPAEYIEALYKK
                     ALVLSQTDPEGALGVLRQLLEEDPQNTRARALLARIEGRLNRERWAQSDIPVAPAALR
                     AYADGIVFYNTGQITEAKAAFALALNLAPNFERAAVALRKCESYSKGARF"
     gene            complement(5293..>5534)
                     /locus_tag="PLF85_11510"
     CDS             complement(5293..>5534)
                     /locus_tag="PLF85_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20339.1"
                     /translation="DLDLAWSRTPQPVGNRYHKAMPTPEPGSEVASRFEALEPQKARV
                     IVRNNRSDSGALRNTQAGVLGFGQKPVFQNRAPLE"
BASE COUNT         1294 a         1536 c         1544 g         1160 t
ORIGIN      
        1 atgcccaggg gcggatgttc ggcgccgtgc gcgccgtcat ttgccctgtg cgaggagctc
       61 ttggatgact tcataatctt tttcgatacc ctttcggaaa gacgaagcga tctggttttc
      121 gagcatacca ccgaccagcg ccatattcac cttcacggtg atgtcgtatt cgcgccgcga
      181 gctcgtatcg ctttcgtgga cgtagtgggt aactccgctg atgcgcaacc ggtcaggatt
      241 ctgctcatag acaacttcaa accggttaat cttgtttttc gtatcgaact tcgccgtctc
      301 gaccaggttc agaaaacctg ccggaatcat ggccttcagc gcttcgggga tcttgtccgc
      361 gagcgagaat gcacggcgcg aaacaatcag gtcgccttcg tgcttgcggt cgagtagttc
      421 ctgctttttc agcgattcct gtttcgagac gtcatcaaaa cgccgctcac gggctgcaag
      481 tacctcgtcg acgtgggcag aaaaactttg tttcagtttg aagtttgcgg gcatagttta
      541 gcgctgcata atggaaagcc gaggctaggc ggaaagagat ttatggccct tcgggcccct
      601 gcctgcaggc ccgttagtct tgacgctaca cgcaagatgg gcccgcctgg tgccgaaact
      661 gggattatgg caggaagcga aagaattctt atacttgaag atgacctggc ctttgcccgg
      721 ctgatgggcg aagtcctgaa agacctgggg cagacccggg ttacttcgta ccccgagcgc
      781 ctgccacagc tgatcgccga gttcgcgccg actctgctga ttgtcgacta caacctcaac
      841 catcctacca tgaacggtat caaggcgacc cggctggtga agcagaaccc tgaaacggcg
      901 ctcgtgcctg tcctcttgct ctctggtgaa gacgacctgc aggttattga agaagctttt
      961 aagtcgggta ttgaagatta cgtgctcaaa ccggtgatac cgcggtttct cattgcgaag
     1021 attgagaata tcctcttcca gacccgccgc aagctgaatg cccaggcgct ttccggactg
     1081 ccgggtaacg cggccattga gacggagttc tatctcaggc tacagaaaaa gaaaccgttt
     1141 tcggcggcct ataccgacct cgaccacttc aaacctttca atgacgaaaa gggagttaaa
     1201 aaaggagatg atgccatcca gtgcgtggcg aaaattcttt atggactgcg ggccaattcg
     1261 tcgcgtgaac aactgtttgt cggccatatc ggtggcgacg attttctgct gtttggcagc
     1321 aagactgccg tcgctgctgc cgcgcgcagg ttgcgccgcg attttcgccg cgagacgcgc
     1381 gtctttttta cggctgctga attgcgcgcc ggtgggtacc gtggcaccga caggcaggga
     1441 agttaccgct ctttcccact gctcgacatt tcgactgcca tcatcgaccg tattactgcg
     1501 gatacagtcc ccgatttttc ccggctgacc gaactggcag cgcgggcgaa aaaagccgca
     1561 aaaaattcga ccacccacgt tgcggcgttt gacgccggtg aactcaaatt gcctggtgac
     1621 gcgaaaacac tctttgtcca agagccaggc agtgccaaaa aaaatacggc aactgcgcgc
     1681 cgtgctgtaa aataatgctc tgccgcctga acgaaatacc gaaacttact atagggtttc
     1741 accatggaat ttgccaaata ccttatcgat tttctggtct atcttgctct cgccgcggcg
     1801 attagctggt tcttcttctt tttcaagcgg ttggatcttt tcggcggttt tgtgggcggc
     1861 gcggtggtgg cgctcattgg ctccattctg ggggcatttc tgctccaggg actgctgtca
     1921 gatattatca agttcctgca gaatggcctt aatatcagca acgtcaatat catcgcggct
     1981 gtgcttggtg gtacggtcag cgtgatgctg ctcagcaaaa ttaataacgg caggcagcgc
     2041 aaagactact gaaattgcgt ttttcccctg ttcttttttc catccttttt gcctgtaacc
     2101 gccaggacgt tccgttgagc gaccttgcaa taaaaataac cgaatccgag tatactccct
     2161 ctggcgatgg ctgggcagaa gcgcgccgca gacgactcgt cacacgctat ctcgaagcga
     2221 ggggatttca ggtgcgtgcc gtacagcgcc cgcacgatga tggtcggccg gccagtaaat
     2281 acaggcaggc agacctggtc gcgcagcgcg gagcagtgac gccagagggc aggcgcgcca
     2341 gggcgacact ctcgctgcta ctgccagaga agctcccggt ttcctcggat tttgtaaaac
     2401 gccaaaaaca gtgcgacagc gttttacttt cggtgctgga aaaagccgca gagttcagcc
     2461 aggttgagct cgtatcatcg ccggtatgct tcggtttgac agcgcagggc gaagcgcagg
     2521 agtaaacaat gaaagaaatc atcgtcgcgg ccccacgcgg attctgcgcg ggcgttgacc
     2581 gtgctatttc aatcgtcgaa aaggctctcg aggtttacgg tacgccgctg tatgtacacc
     2641 atgaaattgt gcacaaccgg catgtggtag aaggactgcg cgccaaaggc gtcatcttcg
     2701 tcgacacaat cggtgaaatt cctggcaacc agacggtgat tttcagcgcg catggggttt
     2761 cgcccgcagt cgtttcagaa gcaaaacaga agaacctccg catcctggat gcaacctgcc
     2821 cactggtgac caaggtacac atcgaagccc ggcgttacgc gcgcgaaggt tatcgtatca
     2881 tactgattgg ccataaagac catatcgaag cgcgcggtac gcacggcgaa gccccagacc
     2941 agatgacaat cgtcgaaaca gcggaagata tcgataagct gcagatcgaa cccggggcga
     3001 aggtggctta tctgacgcag accaccttgt cggtgcgaga tacgcgcgga ctcattgaat
     3061 tgctcaaggc caggtttccg cagattgaag gtccaagttc aagcgacatc tgttatgcca
     3121 cgacaaaccg gcaggaagcg gtgtcggcta tggcgccaga gaccgacgtg gtctttgtga
     3181 tcggctcgcg caactcgagt aattccaaca ggctgaaaga actcgccgaa tcagaaggca
     3241 gccgcgcgta cctgatcgac agcgcggccg acattctgcc tgagcacctt gccggcgtgg
     3301 tcaagggcat cggtatcacc gcaggcgctt cagcgccgga aattttggtg cagcaggtta
     3361 ccgagcggtt gcagaaagaa tttggttttg cctctgtgcg tgaactgcgt ataaaagaag
     3421 aggacgttat cttcaagctg ccgaaagaac tggctgctca cggctgatgc ggagttttct
     3481 cgttctgatt ctgttgccgc tcccgcttgc ggcgcagacg tatgacgagg taaaaaacct
     3541 gaccaaggcg ggtattgccg cctaccacca gaagcgtttt gccgaagcga aagacagtct
     3601 ttcagccgcc ctcgatattg attccacctg ggtacccacc gttctgcacc atgggctcgc
     3661 ctatgctgac tggctgacag ctcagattca tatacgcaat acccgcaagc tgattgtctt
     3721 cgaaaaatac gagcgtgcag accgtgaact cgacgaggcc gaggcgctgt atcccgagca
     3781 cccggaaatt ccgccgctgc gtgacctgat caggaaaaaa cgcagcgaca ataccaaggt
     3841 aatcctcgag aagctaccgg acaaaaaacg caaagaatac gaagagagta tgcagaaggc
     3901 gcaggttgcc atggacgcgg gtaaataccc cgaggcgatg caccactaca gcacggcgct
     3961 caaggttgct ccggactcgc tcgatgcgaa gatgggttat gccgaagccg agcgtatgct
     4021 gaagcagcag ggtagtgcac agaagctgac cgtacttttc cggcaggccg aaaaatatga
     4081 aaaagaacgc cgcttcgcgc aggcagtgtc tgtctacgac cagatattgc gcatcgagcc
     4141 ccagaacagg caggcaattg aacgccgcgc tgcgctgatc gacttcatgc agcagcagct
     4201 gggcaagaac gagcggcgca atcttgcgaa agagtacctg cagagcggta atgaatcgtt
     4261 tcgcaaggca gagtacgacc aggcgatcga ccagtaccgt atcgggcagg cgctcgaccc
     4321 gaagtttgcc gactgggatg ccctgattaa gaaagctatg gctgcacgcc gcgcgcagga
     4381 agaaaacctg ttcgccgaaa aacttaagga gctcgagcgg cgctaccagt cggcgatggt
     4441 gcagttactg ctcgagaact accccgcggc gatcgaagac cttgaagcgg tgatcgccat
     4501 cgcgcagcag ttcaagcagg ccgagacaca gaaacaggcc gaggcgctgt tacaaaaagc
     4561 caaagagtca atgctgcggc aggacgaaga attcatcacg cgtgattcgc cgtattatgc
     4621 cttcgtacaa tcgctgacca cgctggggct taccagctac cggcagcgcg attgccaggc
     4681 gacgctcaaa catttcggtg cgattaccga ggtctttccg aaaaaccgcc tgtcgaacca
     4741 gcatattctt tcgtgcacaa tcatcctcaa ccccgagcga aaagacggta ttgtgcgcga
     4801 tctcatcgaa gcgatttacc gcctgaaaga taccaacgcg tacgaagcac gccgcctgtt
     4861 cgagatactg aagtttatcg atccgcagaa cccggctatc gcgcagcttg agaaagaact
     4921 cgcagagaag acaacgctgc tgaagaaggc agtgcagccc gcagaatata tagaggccct
     4981 gtacaagaaa gcgctcgtac tctcgcagac agaccccgaa ggtgcgctgg gcgtactgcg
     5041 ccagcttttg gaagaagacc cgcagaacac gcgcgcccgg gcgcttctcg cgcgtatcga
     5101 aggacgcctc aaccgtgagc gctgggcgca gtcagacata ccggtggccc ccgccgcgtt
     5161 gcgtgcgtac gccgacggca ttgtctttta caacaccggg cagattaccg aggccaaagc
     5221 cgcgttcgcg ctggcgctca accttgcgcc caactttgaa cgcgcggccg tcgcgttgcg
     5281 taagtgtgaa tcttactcca agggagcacg attctgaaat actggcttct gaccgaaacc
     5341 caatacgccc gcctgggtat tgcgcaacgc accggaatcg ctgcgattgt tacggacgat
     5401 tacacgagcc ttctgcggct cgagcgcctc aaatctgctc gcaacctcac tccctggctc
     5461 aggcgtcggc atggccttgt ggtaacgatt gccgaccggt tgcggggtgc gcgaccacgc
     5521 caggtcgaga tcca
//
LOCUS       DAOQJF010000220         5503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8554_length_5503_cov_4.955947, whole genome shotgun sequence.
ACCESSION   DAOQJF010000220 DAOQJF010000000
VERSION     DAOQJF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8554_length_5503_cov_4.955947"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLF85_11515"
     CDS             <1..190
                     /locus_tag="PLF85_11515"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801536.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNE20340.1"
                     /translation="AVYCDGEYGYKDMHIGLPVVLGKNGVEKIIEIKLTAEEKAALDK
                     SAEAVEGLKADLKRLNFL"
     gene            331..1419
                     /locus_tag="PLF85_11520"
     CDS             331..1419
                     /locus_tag="PLF85_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20341.1"
                     /translation="MSMRRAIYLILALAFLPLRRLAAETVCGPVRVAAPSAVTAGLDQ
                     VAVETEVCNQVNSKFQTGNFSGILSYMAKAQSVASAGRVADYASNMTVFSLGAGTTVA
                     VSNVGLPTSSGDLDALNKRLSSATVPDLGSGFSASATLGVSLRTTNLRRRGFFDPKKL
                     NIYLSFFVLPTLAYSGYTINMTSGSLYLQYKLLQPRRVPLALLTWGGLDLGLGYTYAS
                     SKFVAASTDKLASISFVYNSQNILYEPSGSLSVAYTTHVIPLELSTNVSLLHFLSLVV
                     GAAADFHLLAQADLTADVSGAVKVGGFTTTGDYARFNTTETGRAEAVVLRAFGGPQFN
                     IWKVRVFTLVHATNNQTYGLTMGARFTW"
     gene            1413..1922
                     /locus_tag="PLF85_11525"
     CDS             1413..1922
                     /locus_tag="PLF85_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20342.1"
                     /translation="MVKNVLLIVLALGACSRPFVMKPKEFQKPVLVGPVRQIGGEKVA
                     VDASQNYPFMTEIEILDTPVYPSHDWHVKQERKLDDKLTAASAFGSRDTVIDEMRLGS
                     YVFNIVLFYLDKDWIQLRGHSETALYTEPERRYILNVAPARGGKKSRFERTEKKSRPE
                     RRLTRRRRR"
     gene            1919..2344
                     /locus_tag="PLF85_11530"
     CDS             1919..2344
                     /locus_tag="PLF85_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20343.1"
                     /translation="MIRLVSFALVLVLLQACVGSRHTVKFQELEYPVSMSGQLYGSDK
                     RMKADGAGLDIIGDVEVTRRYWSLLYSFAPLGDTTQQVRDLNRVIAARGAQGVVHFEI
                     ENEGCDMNNFALLVAPAVLPFFPGCSKITLRGKLVRESR"
     gene            2415..2840
                     /locus_tag="PLF85_11535"
     CDS             2415..2840
                     /locus_tag="PLF85_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20344.1"
                     /translation="MAKPTKRQPKTVKKKATTAARGKTSPGKKKSTPKDARTLVQERR
                     HESRKLITRATFVGFSFDLSYLEIPGALFVPQLNALIVDQSEQGCSLVFLKGNPLTAN
                     LIVGAHCLVQIEPDRTLKAVIRWVKELDNRLVNAGFEFV"
     gene            2895..4316
                     /gene="gatA"
                     /locus_tag="PLF85_11540"
     CDS             2895..4316
                     /gene="gatA"
                     /locus_tag="PLF85_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNE20345.1"
                     /translation="MLSELARDLAAGKTTAVSLLEAALAKIEKHDGEIKAFLAVEREQ
                     ALAAAKASDERRAQGKALSPYDGIPIGIKDNIVTKGRVTQCASKILDGFVSPYTATVI
                     EKLQAQGFVTLGRLNMDEFAMGSSTENSAFQVTRNPFDTSRAPGGSSGGSAAAVAAGF
                     LPVTLGSDTGGSIRQPAAFCGLAGLKPTYGTVSRYGLVAFASSLDQIGPLAHTVADVE
                     IIHNIMKGHDPKDSTTMPDSSYTKWSGKLSDVRLGIDEKMLEGLTPEVRASFDKTLAF
                     LKGKVKSVESVSLPNQKFGIPVYYLTATSEASANLARFDGIRYGHRTDNAKNLNEVYE
                     KSRTEGFGPEVKRRILLGTYALSSGYYDAFYGKAQAVRALIQQDYNEAFKKVDAILMP
                     STPSPAFKIGEKTSDPVQMYLSDVLTVGASLAGVPALAVPGPAEGLPIGMQLQGPNFS
                     EELLFELGKAIESEFPQKRVELK"
     gene            4323..>5503
                     /gene="hisS"
                     /locus_tag="PLF85_11545"
     CDS             4323..>5503
                     /gene="hisS"
                     /locus_tag="PLF85_11545"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNE20346.1"
                     /translation="MAYLSTQPYKGTRDFFPEDMRFREAMFSVMHRVVRQYGFDQIDA
                     PIVEPIDVYLAKTSEEIVGQQIYSFTDRGDRKVAIRPEMTPTVARMVAARFHETPKPI
                     RWYSIPNVWRYEQPGKGRLREHWQLNCDLFGAVSEKLGDLEILSLAVDLLRGYGADAS
                     QFRLYLSHRSILNGVLGGELKLPQEKWPAIARIMDKKAKVKPEAYVAMLEAEGVTALQ
                     RAELDFFLLERVDYLKKKYHLEGASYVIELMDELKNLGLDEFVEYDPSVVRGFDYYTG
                     LVFEVFDTHPENRRSLFGGGRYNNLVGAFGKETLNAVGFGMGDVTLEDFLRTHQLLTD
                     QPRKVDLYIALLDEKLGGHALALARKLRARGLSVEVSLGAGKLGKQFQEAEKKQIARV
                     I"
BASE COUNT         1168 a         1562 c         1565 g         1208 t
ORIGIN      
        1 ggctgtttac tgcgacggtg aatacggcta caaagacatg cacatcggtc tgccggttgt
       61 tctcggcaaa aacggcgttg agaagatcat tgagatcaag ctgacagccg aagaaaaagc
      121 cgcgctcgat aagagcgccg aggctgtcga aggtttgaaa gcagacctga aacgtctcaa
      181 ttttctctga gtcgcgtttc agcatggccc cagtgccagc gccagaaggg ggagtaatcc
      241 ccctttttta ttgccttatc ccgtaatgga cgatcttcac cgaacacaaa tcgcatggct
      301 tcacttgggc gcagggttac ccgataatta atgagcatgc gccgcgcgat ttatcttatt
      361 ctggcacttg catttttgcc cctcagacgc ctggcggccg agacggtctg tgggcccgtg
      421 cgcgtggccg ccccctctgc agttactgcc ggacttgacc aggtggctgt ggaaaccgag
      481 gtctgcaacc aggttaactc caagttccag acgggcaatt tcagcggtat actgagttac
      541 atggccaagg cgcaatcggt ggcgtctgcg ggacgcgtgg cggactacgc gagtaacatg
      601 acggtctttt cgctcggcgc cggcacaacc gtcgcggtga gcaacgttgg cctgcctacg
      661 agcagcggcg atctcgatgc gctgaataag cgattgagta gcgcgacggt gccagatctc
      721 ggttcgggct tttctgcttc ggcgacactg ggtgtcagcc tgcgcacaac caacctgcgc
      781 cgccggggtt tttttgaccc gaagaagctc aatatctatc tctcgttctt cgtgctaccg
      841 actctagcct attctggtta cacgatcaac atgacttcgg gtagccttta cctgcagtac
      901 aaactgctgc aaccccggcg tgtgccattg gcactgttga cctggggtgg tcttgacctg
      961 gggcttggct acacttatgc gtcgagcaag tttgttgccg cgagtaccga caagctcgcg
     1021 agtatcagtt tcgtctacaa ttcgcagaat atcttgtacg aacccagcgg cagcctgagc
     1081 gttgcctaca ccactcatgt gattccgctc gaactttcga ccaacgtatc gttgctgcat
     1141 tttctctcgc tcgtagttgg tgccgccgca gactttcacc tgctggcgca ggccgacctg
     1201 acggcagatg tttcgggtgc agtgaaagtc gggggcttta ccactaccgg cgactatgcg
     1261 cgattcaata cgacggagac cggccgagct gaagctgttg tcctgcgtgc gtttggtggt
     1321 ccgcagttca atatctggaa ggtgcgggtc tttaccctgg tgcacgctac taacaaccag
     1381 acgtacggcc tgaccatggg ggcgcgtttc acatggtaaa gaatgtgctg cttatcgtgc
     1441 tggctcttgg cgcctgctcg cggcccttcg tcatgaagcc aaaggagttc cagaagccgg
     1501 tgttggttgg cccggtgcgg cagattggcg gcgaaaaggt ggcagtcgat gccagccaga
     1561 actatccatt catgacggaa attgagatac tcgatacacc ggtatatcct tcacacgact
     1621 ggcatgtgaa gcaggagcgc aagctcgacg acaagctgac cgccgcttcg gccttcggca
     1681 gccgcgacac ggttatcgac gagatgcgcc tgggcagtta tgtcttcaac attgtgctgt
     1741 tttatctcga caaggattgg atacagctgc gcggccattc agaaacggcg ctctacaccg
     1801 agcccgagcg gcgttacatt ctgaacgtgg caccggcccg tggtggcaaa aaatcccgct
     1861 tcgagcgcac agaaaaaaaa tcgcgccccg agcgcaggct gacccgccgg aggcgccggt
     1921 gattcgcctt gtttctttcg cgctcgtcct tgtgctgttg caggcctgtg tcggcagccg
     1981 gcacacggtg aagttccagg agctggagta cccggtgtcg atgagcggcc agctctatgg
     2041 ctcggacaag cgcatgaagg ccgatggcgc tggcctggat atcatcggcg atgtggaagt
     2101 cacgcggcgt tactggtcgc tgctctatag ctttgcgccg ctcggtgata cgacgcagca
     2161 ggtgcgcgat ctcaaccggg taattgccgc ccggggcgcg cagggtgtag tccactttga
     2221 aattgaaaac gaagggtgcg atatgaacaa ttttgcgctg ctcgtcgccc ccgcggttct
     2281 gccgtttttc cccggttgca gcaagataac cctgcggggc aagctggtcc gggagtcccg
     2341 gtagggaatg ggcgggcaga gctattttcg aaacctattg acggcgctct cgcggccact
     2401 catctggccc ctacatggca aaaccgacca aacgccagcc taaaaccgtc aaaaagaagg
     2461 cgaccaccgc agcgcgcggt aaaacttccc cgggcaaaaa gaaatccacc cccaaagatg
     2521 cccgcacact ggtgcaggag cgccggcacg aatcgcgcaa actgatcacg cgcgccacgt
     2581 tcgtgggttt ttctttcgac ctgtcgtacc ttgaaatacc cggtgcgctg tttgtgccgc
     2641 aactgaatgc gctgattgtc gaccagtcag agcaggggtg cagtctggtt tttctcaagg
     2701 gtaacccgct taccgccaac ctgattgtgg gagctcactg cctggtgcag attgaacccg
     2761 accggacgct caaggcagtc atccgctggg tgaaagagct cgataaccgg ctcgtcaatg
     2821 ccggctttga gtttgtctag ccctgccggc actgaggtgc tttacgtcgg gtctcgcacg
     2881 ccgtattagc tcccatgtta tctgagctcg cccgcgacct ggccgcagga aaaaccaccg
     2941 ccgtctcact gctcgaagct gcactggcaa aaatcgaaaa gcacgacggc gaaatcaagg
     3001 catttctggc agtcgagcgt gagcaggcgc tggcagccgc gaaagcttcg gacgagcgcc
     3061 gcgcgcaggg taaggcactt tcaccttacg acggtattcc gattggcatc aaggacaaca
     3121 tcgttacgaa gggccgcgtg acacagtgcg cctcgaagat tctcgacggt tttgtttcgc
     3181 cttacactgc tacagtgatc gaaaaactgc aggcacaggg tttcgtcacc ctcggccgcc
     3241 tcaacatgga tgagttcgcg atgggctcgt cgaccgagaa ctctgccttc caggtgacgc
     3301 gcaatccatt tgataccagc cgcgcaccgg gtggttcgtc gggcggttct gccgccgcag
     3361 ttgctgcagg cttcctgccg gtaacactgg gctccgacac aggcggatcg attcgccagc
     3421 ccgcggcttt ttgtggtctt gcaggtctga agcccaccta cggtaccgtt tcgcgttacg
     3481 gcctcgtggc ctttgcttca agcctcgacc agattggccc gcttgcgcac accgttgccg
     3541 acgtcgaaat catccacaat attatgaaag gccatgaccc gaaagattcg acgaccatgc
     3601 ccgattcttc gtatacaaaa tggagcggca agctgagcga tgttcgcctg ggtattgatg
     3661 aaaaaatgct cgaaggctta acgcccgaag tgcgtgcttc gttcgacaag acgctcgctt
     3721 ttctcaaagg taaggtgaaa tctgttgagt cagtctcact gcccaaccag aagttcggca
     3781 tcccggtcta ttacctcact gcaacgagcg aagcatcagc aaaccttgcg cgcttcgacg
     3841 gcattcgcta cggccatcgc acggataacg cgaagaacct gaatgaggtg tacgaaaaat
     3901 cgcgcaccga aggtttcggg ccagaggtca agcgccgtat tctgctcggc acctacgcgc
     3961 tctcgagcgg ttattacgac gccttctacg gcaaagcgca ggcggtacgt gcgctcattc
     4021 agcaagacta taacgaagcg ttcaaaaaag tcgatgcaat tctgatgcct tcaacgccat
     4081 cacccgcgtt caagatcggt gagaaaactt ctgaccccgt gcagatgtac ctgtccgacg
     4141 tgctcacagt gggagctagc cttgcgggtg taccggcgct cgcggtgccg ggtcctgccg
     4201 aaggcctgcc cattggcatg cagctgcagg gaccaaattt ttccgaagag ctgctgtttg
     4261 aactgggcaa agcgatcgag tctgaatttc ctcagaagcg tgttgaactg aaatagctcc
     4321 aaatggccta cctttctacc cagccctaca agggcacacg tgattttttt cccgaagata
     4381 tgcgctttcg cgaagcgatg ttctcggtga tgcaccgcgt ggtgcggcag tatggattcg
     4441 accagatcga cgctccaata gtcgagccca tcgatgtcta ccttgccaaa acttcagaag
     4501 aaattgtcgg ccagcagatc tactctttca ctgaccgcgg cgaccgcaag gttgctattc
     4561 gtcccgagat gacgccgaca gtcgcgcgca tggttgccgc acgttttcac gaaacaccca
     4621 agccgattcg ctggtattcc atacctaacg tctggcgcta tgagcagccg ggcaaaggcc
     4681 ggctgcgcga gcactggcag ctcaactgcg acctgtttgg cgcggtgtca gagaaactcg
     4741 gcgatctcga aatactttcg ctggcagtcg atcttctgcg cggctatgga gccgacgcct
     4801 cgcagttcag gctgtacctc tcgcaccgct cgattctgaa cggtgtgctc ggcggtgagc
     4861 tcaagctgcc gcaggaaaaa tggcccgcaa ttgcgcgcat catggacaag aaggcaaagg
     4921 tgaagcccga ggcttacgtc gcgatgcttg aagccgaagg ggtaacagcc ctgcagcgcg
     4981 ccgaactcga ttttttcctg ctggaaaggg ttgattacct caagaagaaa taccatctcg
     5041 aaggcgcgag ctacgtgatc gaactcatgg acgaactgaa aaatctcggg ctcgacgagt
     5101 ttgtcgagta cgatccctct gtcgtgcgcg gttttgatta ttataccggt ttggtatttg
     5161 aggtttttga tacgcacccc gaaaaccgcc gttcgctttt tggcggtggt cgttacaata
     5221 atctcgtcgg tgcctttggc aaagaaaccc tcaacgccgt tggttttggt atgggcgacg
     5281 tcacactcga agattttctg cgcacgcacc agctactcac cgaccagccg cgcaaggtcg
     5341 acctctatat tgcactgctc gacgaaaaac tcggtgggca tgcgctggcg ctggcgcgta
     5401 agctgcgcgc gcgcgggctc tcggttgagg tttcgctggg ggcaggtaag cttggcaaac
     5461 agttccagga agctgagaag aaacagatcg cacgcgtcat tat
//
LOCUS       DAOQJF010000221         5479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8598_length_5479_cov_5.240229, whole genome shotgun sequence.
ACCESSION   DAOQJF010000221 DAOQJF010000000
VERSION     DAOQJF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8598_length_5479_cov_5.240229"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLF85_11550"
     CDS             complement(<1..208)
                     /locus_tag="PLF85_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20347.1"
                     /translation="MAFPKKSPVRETGRKEHTVIMPHQPRALARGWWVLVLLTIYLLD
                     SIFSIMKRENKTQYALLGILTQYEC"
     gene            202..462
                     /locus_tag="PLF85_11555"
     CDS             202..462
                     /locus_tag="PLF85_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20348.1"
                     /translation="MPPKDNVPPQPPGSTRITLVLERRSDRLDGILLEAMRQQTDNKK
                     LRDISRSALKALFSAHKILIKGQPARPSSSIATGTTYVDVLI"
     gene            complement(378..1025)
                     /locus_tag="PLF85_11560"
     CDS             complement(378..1025)
                     /locus_tag="PLF85_11560"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNE20349.1"
                     /translation="MRDPYTSGHERRVGSIAADIAREMGWSDARCQQLQWAGMVHDIG
                     KIAVPAEILSKPTRLTQLEYDLIKGHADQGYQILKDLEFPFPIAEIIRQHHERMDGSG
                     YPRGLKSGEILPEARILAVADVLESMSSHRPYRPALGINAALDEIKKGRGTHYDEQVV
                     DAFIRLVEEKGYTLARDVQFDRFALQTRQISTSTYVVPVAMLDDGRAGWPLIRIL"
     gene            complement(1579..2067)
                     /locus_tag="PLF85_11565"
     CDS             complement(1579..2067)
                     /locus_tag="PLF85_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20350.1"
                     /translation="MKILNRRAIPVITLALCSTFVLQATQETVIQSEARQFGERAIAK
                     GYQCRTEVHQTTGKLFEVKMPMDKNILYHIAVVTGRGEKSAHIKSAILVNEERMNIKI
                     EVQNTIFGSELKMHPLVTGPKTLKFELDAKSSYSVVVCANYASLFHTEEKDRVIDDHS
                     HF"
     gene            2259..3431
                     /locus_tag="PLF85_11570"
     CDS             2259..3431
                     /locus_tag="PLF85_11570"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNE20351.1"
                     /translation="MRTTFGIDRLIADPGKYLKGKRFALLVNQSSISSDGRYLFEALG
                     AQGYKAEKIFAPEHGLFGTEQDQITVADEVDAFTGLKAVSLYGQTKEELKPRSDDLEG
                     IDALVVDIQDIGCRYYTYAYTMAFCIEACAARGIEVIVCDRPNPLGGVVVEGNVVHPG
                     FESFVGAYPLAVRHALTIGEVARFLNATQKWQAKLTIVELQNWRREQLYPTTGGLWSQ
                     PSPNMPTFDTTVIYPGTCLFEATNVSEGRGTTRPFEIIGAPFVDPKEYAAALNAQKLP
                     GVYFRPLYFKPTFHKFKDEACGGVFVHVTDVTVFESFYTGLAMVKTAHDLYPNEFDWR
                     HEPYEYVTDKLAIDILSGSAQFRLNVERDESLADYRNAYIGEARDFHKQAVDFFLY"
     gene            3607..3843
                     /locus_tag="PLF85_11575"
     CDS             3607..3843
                     /locus_tag="PLF85_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20352.1"
                     /translation="MKKTVSLLSVLALLALVAPAFAQDAQPAPAPAPAADCKALKGKE
                     KAACVKAEKKAKADAKKAEKAAKKAEKAEKKAAK"
     gene            4032..5444
                     /locus_tag="PLF85_11580"
     CDS             4032..5444
                     /locus_tag="PLF85_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNE20353.1"
                     /translation="MAFVDLIVLFGYFALLQLVGFAASRRRNHAADYLSGSHSLPWWV
                     VSASIVAAETSSLTFISIPGLAYAGNLTFMQLALGYVLGRIIVAFVLLPAYFKGNIET
                     VYAFLEHRFSVNMRRLAAVLFQVTRLLGTGVRLYATALPLSLISGWSITTSILVFTLL
                     TVPYTSFGGLRAAMWVEFIQTAVYLAGAFAAVFVLQVSGVPLESAGTEKFRFFASGLE
                     NGLVSFWTQPFHLTAGLIGGALLAISSHGTDQLIVQKALACRNLRDAQKAMITSGILV
                     DLQFLLFLIIGVWLFAFFKGATFATPDAVFPTFIISQLPAGLIGLVLAAIFATGQSTL
                     SATLSSLASSTMLDLFPGYAAHDDKRRLRLSRLLTVVWAFAIMLIALLFTDQKNPVVV
                     VALKLSSILAGGIVGLYVLGFRRTSQRAALVGFSTSATAMGAIAAFTPLAWTWYVPAG
                     LAICLSVAGCVTLVSRLWRR"
BASE COUNT         1136 a         1543 c         1486 g         1314 t
ORIGIN      
        1 tacactcgta ctgcgtcaaa ataccgagca gggcgtactg cgtcttgttc tcgcgcttca
       61 ttatcgaaaa gatactatct aatagatata ttgtcaataa aacaagtacc caccaaccac
      121 gggctaaagc ccgtggttgg tggggcatga tcaccgtatg ttctttacga cctgtttctc
      181 ttacgggact ttttttggga aatgccaccg aaagacaatg taccgcccca gccgcctggc
      241 tcgacgcgta tcaccctggt actcgaacgg cgcagcgacc ggctcgacgg cattctgctc
      301 gaagcaatgc gccagcagac tgacaacaag aagctgcgcg atattagtcg ctcagcgctc
      361 aaggcgcttt tttctgctca caagattctg attaaaggcc agccagcgcg cccgtcatcg
      421 agcatcgcca ccggcaccac gtatgttgat gtactaattt gacgggtttg cagggcaaac
      481 ctgtcaaatt ggacgtcacg agcgagtgtg tatccctttt cttctaccag gcgtatgaat
      541 gcgtcgacta cttgctcgtc gtaatgtgta ccacggcctt tttttatctc atcgagcgcg
      601 gcgttgatac cgagcgcggg gcgatacggg cggtgcgagc tcatcgattc gagtacatca
      661 gccacggcga gaatgcgcgc ttctggcaag atctctccac tctttagacc gcgtgggtac
      721 ccgctgccat ccatacgttc atggtgctgc cgaatgatct cggcaatggg aaacgggaat
      781 tccaggtctt taagaatctg gtaaccctgg tcagcatggc ccttgataag gtcgtattca
      841 agctgcgtca ggcgggtcgg cttggagagt atctctgccg gcacggcaat cttgccgatg
      901 tcgtgcacca tgcctgccca ctgcaattgc tggcaacggg cgtcgctcca gcccatttcg
      961 cgcgcgatgt cggcggcgat agaacccacg cggcgctcgt gacccgaagt gtacgggtcg
     1021 cgcacctcga ccacattggc tccgtcgata acttgaagag cttgaggtag tgttctttcg
     1081 cctgctctac ctctttggcg cgcgttaggt cccggacgat catgaccgtt cgtagctgac
     1141 cgtcttcatc ggtaaaaacc tgcgagctgc tttccccggg aaagcggctg ccgtcgcccc
     1201 gaaagaagaa catttccgca cgtgccctgc cggtgtgttc acgctcttcg aaaaatgccg
     1261 gtaacaatgg agatttcgga tccacaagac cagcgcgacc cacccggcag atctcttctt
     1321 cgctgcgtcc gaaaagcttg caggcggcag ggttcgcggc gagaattgcc ccatctggcg
     1381 cagtcaaaaa cactgcatcg agactgaact caaagaacgc gcggtaaaag ctatcgggat
     1441 gttttttttg ccgccggtcg cggacatttt ttcattttgt gccatatgcc agggtgcacg
     1501 gttgcaccga atcagggaag ttaggagttt ttgggtggtg ggtggcaccc acatacccaa
     1561 agaggtaatg cgcgtaaatt agaaatgcga atggtcgtcg atcacgcggt ctttttcttc
     1621 cgtgtgaaac agcgacgcat agttggcgca gaccacgacc gagtagcttg atttggcatc
     1681 gagttcgaat tttagggtct tcggccctgt aaccagcggg tgcattttca gctcagagcc
     1741 aaagatggta ttctgcacct cgatcttgat gttcattctt tcttcgttta cgagaatcgc
     1801 gctcttgata tgcgctgatt tttcgccacg accggtgact actgcgatat ggtacagaat
     1861 gttcttatcc attggcattt tgacctcgaa aagtttgcct gtcgtctggt gcacttcggt
     1921 gcggcactgg taacccttcg caatcgcccg ctcgccaaac tgccgcgctt cgctctgtat
     1981 caccgtctct tgggttgcct gcagaacaaa cgtgctacag agcgccaggg taataacagg
     2041 tatcgcgcgg cggttgagga ttttcatcgg ttacgggtcg ggtgtcgatt tcatggctgc
     2101 aatcgtattt tggaacagat tgagcccctg tgtaagacgt catccaactc agcgggcctg
     2161 accagcgcgc gaaggaactc cggtatttct atctgttcgg cggctttgaa tctgcttgcc
     2221 gcgctgatgc cgcggattga cctgcagcct tctctgacat gcgcaccacc ttcggcatcg
     2281 accggctgat tgccgacccc ggtaaatacc tcaaaggcaa acgcttcgct ctgctcgtga
     2341 accagagctc aatctcatcc gacggcaggt atctgtttga agcgctcggc gcgcagggct
     2401 acaaggcaga aaaaatcttt gcccccgaac acgggctgtt tggtaccgag caggaccaga
     2461 tcaccgtcgc cgacgaggtc gatgctttca ccggcctcaa ggctgtcagc ctgtatgggc
     2521 agacaaaaga agagctcaaa ccccgcagcg acgaccttga aggcatcgac gcgctcgtcg
     2581 tcgacatcca ggacatcggc tgtcgttatt acacctacgc gtacacgatg gcgttttgca
     2641 tcgaagcctg cgccgcgcgc ggtatcgagg tgatcgtctg cgaccggccc aacccgctcg
     2701 gcggtgtggt ggtcgagggc aacgttgtgc accccggttt cgaatcgttt gtgggagctt
     2761 accccctggc tgtgcggcat gcgctcacga ttggcgaggt cgctcgcttt ctcaacgcga
     2821 cacagaaatg gcaggcgaag ctgacaattg tcgaactgca gaactggcgg cgtgagcaac
     2881 tctacccaac gacaggtggc ttgtggagcc agccttcgcc caacatgccg acctttgata
     2941 cgactgtcat ttatccgggt acatgcctgt ttgaagcgac caatgtttca gaaggccgcg
     3001 gtaccacccg cccgttcgag attatcggcg caccgtttgt cgatcccaaa gaatatgctg
     3061 ctgccctgaa tgcgcagaag ctccccggtg tgtattttcg cccgctctat ttcaaaccga
     3121 cgtttcacaa gttcaaagat gaagcctgcg gcggagtatt tgtgcatgtc accgatgtca
     3181 ccgtgttcga gtcattctac accggcctcg cgatggtcaa aaccgcgcat gacctttacc
     3241 cgaatgagtt cgactggcgg cacgaaccgt atgaatacgt gaccgacaaa ctggcgatcg
     3301 atatactgag cggctcagcg cagttcaggt taaatgtaga gcgggacgag tcgctcgcag
     3361 attaccgcaa tgcatacatt ggtgaagcgc gtgactttca taaacaagcg gtggattttt
     3421 ttctttactg atacaaaacc gcaggcaaaa ttgggcttcc cgctgcaccg gcagacctag
     3481 tgggattttt tacctacaaa gagtcaatga gcagaaaagc tgaacttggc aaaaaaatga
     3541 ccgcctttag ccccagtagc gtctattaga taagcccgca agggacttaa aaattttgga
     3601 gacaacatga aaaagacagt ttcacttctc tcagtactcg ctctgctcgc gctcgttgcg
     3661 cctgctttcg cacaagacgc acaaccagct cctgctccag cacctgctgc tgattgcaaa
     3721 gcgctgaaag gcaaagaaaa agctgcttgc gttaaagcag aaaagaaagc aaaagctgac
     3781 gcaaaaaaag ctgaaaaagc agctaaaaaa gcggaaaaag ctgaaaagaa agctgcgaaa
     3841 taagtaattc cttacggatt tacctagctg cttcggcagc gaaaaaagcc cttcggggct
     3901 ttttttattg ctctgtcggt ctggcatcag aaaggtctat ttagttaacc acggcgacac
     3961 ggcgggcacg gcacaccaaa tctgtttacg gcttttgcgg tcactgggcg cagtcgaagt
     4021 gaccgcaaat catggccttt gtcgacctca tcgttctctt cggttatttc gcgctgctgc
     4081 agctggtcgg ctttgccgcg agccgcaggc gcaaccatgc cgccgattac ctttcgggca
     4141 gccattcatt gccgtggtgg gtggtgtcgg catccattgt ggcggcggag acatcgagcc
     4201 tgacgtttat ctcgatacca ggtctcgcgt atgcgggcaa cctaactttc atgcagctgg
     4261 cgctcgggta tgtactgggg cgcatcattg tggcgttcgt tttgctgccc gcgtacttca
     4321 agggtaatat cgaaactgta tacgcgtttc tcgaacaccg gttcagcgtg aacatgcgac
     4381 ggctcgccgc cgtacttttc caggtcacgc gcctgctcgg taccggcgtc aggctctacg
     4441 ccacggcgtt gccgctcagc ctcatcagcg gctggtcgat taccacgtcg atactcgtct
     4501 tcacgctgct gaccgtgccc tatacttcgt ttggcggcct gcgcgccgca atgtgggtgg
     4561 agttcataca gaccgcagtc taccttgccg gggcgttcgc tgcagtcttt gtgctgcagg
     4621 taagcggtgt gccgctcgaa tcggccggta cagaaaaatt ccgcttcttc gcgtcggggc
     4681 tcgaaaacgg tctggtctct ttctggactc aaccgtttca cctgaccgcc ggactcattg
     4741 gtggcgcgct gctggcgatc tcttcgcacg gcactgacca gttgattgta cagaaagcac
     4801 tcgcctgccg caacctgcgc gacgcgcaga aggcgatgat cacgagcggc atcctcgtcg
     4861 acctgcagtt cttacttttc ctcatcatcg gtgtctggct gttcgccttc tttaaaggag
     4921 cgacctttgc gacgcccgat gccgtgtttc cgacgttcat catcagccag ttgcctgcag
     4981 gcctgattgg cctcgtgctc gcggcaattt tcgcgaccgg ccagtcgaca ctttcggcga
     5041 cgctgagttc gctcgcatca tcgacgatgc tcgacctttt tcccggttat gcggcccacg
     5101 atgacaagcg gcgcttacgc ctttcgcgcc tgctcactgt tgtctgggct tttgccatca
     5161 tgctgattgc tctgctcttc accgaccaga aaaacccggt cgtggtggtt gcgctgaaac
     5221 tatcgtcgat tctcgcgggt ggtatcgtgg ggctctatgt gctgggtttt cgccgcacga
     5281 gccagcgcgc ggcactcgtg ggattttcca cgtctgcaac ggcaatgggc gcgattgcag
     5341 ccttcactcc tctggcgtgg acatggtacg tgcccgccgg gctcgcgatt tgcctgtctg
     5401 tagctggctg cgtgacgctg gtcagccggc tttggcgccg gtaatcgaat gtttcttggc
     5461 gagtgttacc tggttaccc
//
LOCUS       DAOQJF010000222         5472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8611_length_5472_cov_4.487355, whole genome shotgun sequence.
ACCESSION   DAOQJF010000222 DAOQJF010000000
VERSION     DAOQJF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8611_length_5472_cov_4.487355"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLF85_11585"
     CDS             <1..368
                     /locus_tag="PLF85_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE20354.1"
                     /translation="EETAQVEKTSAGADVTYVPAVDITEGEAGFTIFADVPGANKDSV
                     SVTFEDGVVTLSAQSAAEAVSSDLIRREFRFANYERAFRVSDDVDAEKISAEIANGVL
                     QVILPRKASAKKTIPVSVK"
     gene            463..1035
                     /locus_tag="PLF85_11590"
     CDS             463..1035
                     /locus_tag="PLF85_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20355.1"
                     /translation="MNITSVRLVVLLLLVFASACTSFERYPTDKSRFTFKGKGVLVAA
                     QDRATGVPRVEALFRQAAGDAAGSIHVLPLMPASAFELPVNAAKIGLQKKGTQGLDPM
                     AQVAVNAILKAGTRFGVKFDYIVFVTAEKANIMGQVINIEYYAALYEISTKRVVAAAR
                     DTGSTTPETAAEQLPLGARRVVSVLLYGDY"
     gene            complement(1049..1963)
                     /locus_tag="PLF85_11595"
     CDS             complement(1049..1963)
                     /locus_tag="PLF85_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20356.1"
                     /translation="MRALLPYLFICLLASPQYAALIVTEEGQVRGQIVRLEEKEVTLR
                     LESGTVQTINKDKILTIHDDDGKLIWSHPSIVVNDTSPDKPGETAKLNLTPQRRPNWE
                     IEATFGLGLMSSTAWFSTYPLGVKHDYRMLTEFNLTGLYNLDGSSYLTASLGYSQRDM
                     TANGVTMEGLYGVAIWPMRFIDARVGYRIREEWLFVEAGLLQTFQVGASPVTIDGGSR
                     STTFSDARASTGGYLGFYVALGIIAQVYRDLEGLFMLRYDHGLSSAVDAKIATQTAPD
                     GTVVSSAPLHLVPWSVSAHVGLRYPLGF"
     gene            2096..2515
                     /locus_tag="PLF85_11600"
     CDS             2096..2515
                     /locus_tag="PLF85_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20357.1"
                     /translation="MQAEQMRAAFLNTVSRRLATLLALPLVLAAAPLCEACPVNTDTA
                     VRAPSTSHAADHACCEKKPQVKQAHKAEHCDNCVAKPATPNSTSELKADLHFSPVTHR
                     AMPEATPVVLPGSTNYITFFYSHSPPAVYLVQQKILV"
     gene            2627..3883
                     /locus_tag="PLF85_11605"
     CDS             2627..3883
                     /locus_tag="PLF85_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE20358.1"
                     /translation="MRYLIFVLLLTSPLLSQESEYYLNLALQNNARLAAERQLASAAQ
                     SDHARESMFLENPELMLGLMNVPVNTFPALNRDSMSNFSVGITQRIALPWEAHYRKAA
                     AELRATTSGLNLELQQAALRYEVMEKLNAIRFYIEREKNLSDAKKLISATMRILAVPR
                     KEARNTAGAILEARAALATAENDLLNNTYELEKAWLDLEALCGTKLNRAEGAGILAAW
                     DKVSATSAEPAHDLRKSLIYRKAESEVRAQQAMVSLSRSALFPEVKVSAAYMFRQGVP
                     GSSMAEDMVAVSASTPLPVFYPLKNKHEVEGQEKRLQAAERMLADTERQLEAQVAAET
                     VRLKTLLASAENYARAILPAHSSAHRSHLANINLMGGSAAEALIAYRMYLTAGEERLR
                     QIREAHSALFKIEYLRAQGVHNQESK"
     gene            3880..4965
                     /locus_tag="PLF85_11610"
     CDS             3880..4965
                     /locus_tag="PLF85_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE20359.1"
                     /translation="MKNLFKSISLFRFLLLIALTAILTDCAKKKEPEVWYCPMHTHYR
                     TDHAGKCPICGMALVKDEAPTKEASAHVAEQHTKKEEAKPAPAATITVSPEQQKLVGI
                     TTAKPQERKLSLTLQLAGQVAYEPDIYAAIIEYRQLAAAAHTLEGSVGGSANLTQSAV
                     LRLRQLGVSSDEIGQYARSETAASRLITGNGGGKALITLRLSEADLALVRKGMAVQVT
                     TPSFPGKVWNGQVTAIGSLVDAKNRSLSARVLVSDRGQLTSQMAVTAELAIRAGSGVS
                     IERSAVLDTGTRQIVFVKVSPTEFEPRAVRVLGGNDDFALVSGITAKDEVAISSAFLL
                     DSEAKLRLGDSVLVSPTRPPPHAGEVP"
     gene            4978..>5472
                     /locus_tag="PLF85_11615"
     CDS             4978..>5472
                     /locus_tag="PLF85_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE20360.1"
                     /translation="MIRRIITFSAENRALTLFLTLVVTLGAWYATKTIRLDALPDLSD
                     TQVIVYTRWDRSPDVVEAQVTTPIVRALMGAPKVKTIRAVTDYGTSFVYVIFDDATDL
                     YWARSRISEYMDRIRTLIPADARLELGPDATGLGWVYQYVLRDKTGKNDLERLRSLQD
                     FTIKY"
BASE COUNT         1211 a         1583 c         1536 g         1142 t
ORIGIN      
        1 cagaagaaac tgcacaggtc gaaaaaacct ccgctggcgc tgacgtgact tacgtccctg
       61 cggtcgatat caccgagggt gaagcgggtt ttaccatctt tgccgatgtg ccaggtgcaa
      121 acaaagacag cgtgagcgtc accttcgaag acggcgtagt caccctgagt gcgcaatcgg
      181 cagccgaagc ggttagctca gacctgattc gccgcgagtt tcgctttgct aactacgagc
      241 gtgcattccg cgtcagcgac gacgtcgacg cagaaaagat atctgccgaa atcgcgaacg
      301 gcgtgctgca ggtgattctg ccgcgcaaag ccagcgcgaa gaagacgatc cctgtttctg
      361 tgaagtaaaa agattcattc agatgcgccc gaaaggcgca tctgaatgaa cttctccatt
      421 ttctcttttc gcaccctcca ttctttatag tctgacgaac agatgaatat aacctcagtt
      481 cgccttgtgg tacttctgct actggttttc gcttccgcct gcaccagttt cgagcgctac
      541 cccaccgaca aatcacgttt cactttcaag ggcaagggtg ttctcgttgc agcacaggat
      601 cgcgcgacgg gagtccccag ggttgaggca ctgtttcgtc aggctgcggg tgatgccgct
      661 ggttcgatcc atgtgctgcc gctgatgcca gcctctgcat ttgaattgcc ggtcaacgcc
      721 gcaaaaatcg gcctgcagaa aaaaggcacg cagggcctcg acccgatggc gcaggttgcc
      781 gtgaatgcca tactcaaagc gggcacgcgc tttggcgtca aattcgacta cattgttttc
      841 gtcactgccg aaaaggcaaa catcatgggg caggtgatca acatcgaata ttacgcggcg
      901 ctctatgaaa tttcgacaaa gcgcgtggtt gcagcggccc gcgatactgg ctccacaaca
      961 ccggaaacag ctgcagagca gttgccactc ggcgcgcggc gcgttgtctc tgttctactc
     1021 tacggcgatt attgattcca acacccggtc agaagcccag cgggtaacgc aggcccacgt
     1081 gtgcactcac gctccacggc acaaggtgta gtggtgcgga gctcaccact gtgccgtccg
     1141 gcgccgtctg cgtggcaatt tttgcatcca ccgccgaact caggccatgg tcgtagcgca
     1201 acatgaacag accctcgagg tcgcggtaca cctgcgcgat gattcccagc gcaacgtaaa
     1261 aacccaaata gccgccggta gatgcccgcg catcggaaaa agttgtgctg cggctgccac
     1321 cgtctatcgt cacaggagaa gctcccacct ggaatgtctg caataggcct gcctcgacga
     1381 agagccattc ttcgcgaatg cggtaaccga cacgcgcgtc gataaaccgc atcggccaga
     1441 tggcgacgcc gtagaggccc tccattgtca caccgttggc ggtcatgtcg cgctggctgt
     1501 aaccgagtga ggcggtcagg tacgagctac catcgaggtt gtagaggccc gtcaggttaa
     1561 actctgtcag catgcggtag tcgtgcttca cgcccagcgg gtaggtacta aaccacgcgg
     1621 tggagctcat aaggcctaaa ccaaatgttg cctcaatttc ccaattgggc cgccgttgtg
     1681 gcgtgaggtt gagcttggcc gtttcaccgg gtttatcggg cgaggtatcg ttgacgacaa
     1741 tcgaagggtg cgaccaaatc agtttgccgt cgtcatcgtg tatggtgaga atcttgtctt
     1801 tgtttatcgt ctgtactgtg ccggattcaa ggcgtagggt aacttctttt tcttcgagcc
     1861 gcacaatctg gccgcgcacc tggccttctt cggtgacaat gagcgcggcg tactgcggcg
     1921 aagcaagcag gcagataaac agataaggta acaaggcccg cacctgtgcc ccttaagctt
     1981 ttgttacacc ctgtcaatta tgtcacacgc agcagatgat cagcagccgg gggttgaaag
     2041 gccatcgccg aaaaaagtga aattttcttt gcagcctgtc gtctttaggt atatcatgca
     2101 ggcagagcaa atgcgcgcgg catttctaaa taccgtctct cgcaggctgg caacgctcct
     2161 ggcgttgcca ctggtacttg ctgcggcacc gctctgcgaa gcttgccctg tgaataccga
     2221 cacagcggtt cgggcgcctt cgaccagcca cgctgccgac catgcctgct gcgaaaagaa
     2281 gccgcaggtt aaacaggcgc acaaggccga acattgcgac aattgtgtgg ctaaaccagc
     2341 cacaccgaat agcacgtctg agttaaaggc ggatctccac ttttcacccg tcactcaccg
     2401 tgcgatgcct gaggcaacgc cggtggttct gcctggctca acgaactaca taacattctt
     2461 ctacagtcat tctccgcctg ctgtctacct ggttcagcag aaaatcctcg tctagcaaat
     2521 acccggcagg ggctttaatg gggtatggcc gaggccatac ctgtcccttc cccgacgtca
     2581 ttcctgcgga agcgggaatc gtatcctggt ctcacggagg atttatatgc gttacctgat
     2641 ttttgttcta ttacttacat caccgcttct ctcgcaggag tcggaatact acctgaacct
     2701 tgctctgcag aacaatgcaa gacttgccgc cgagcggcag ctggcatctg ccgcgcagag
     2761 cgaccatgcg cgtgagagca tgtttctcga aaacccggag ctgatgctgg gtttgatgaa
     2821 cgtaccggtc aacaccttcc ccgcgctgaa ccgcgatagc atgtcgaact tcagcgtcgg
     2881 catcacgcag cgtatcgcgt tgccatggga ggctcactat cgcaaagccg ccgcagagtt
     2941 acgtgccact actagcgggt tgaaccttga gctgcagcag gcggcgctgc gttacgaggt
     3001 aatggaaaag ctcaatgcga ttcgctttta tatcgagcgc gagaaaaacc tcagtgatgc
     3061 caagaagctg atcagcgcga cgatgcgtat tctcgcggtg ccgcgcaaag aagcccgcaa
     3121 cacagccggg gcaattcttg aggcgcgcgc tgcactcgcg acagcagaga atgatctgct
     3181 caacaatact tacgagttag agaaagcgtg gcttgatctt gaggcgctct gtggtacaaa
     3241 gctcaaccgt gccgagggtg caggcattct cgctgcgtgg gacaaagtct ctgccacatc
     3301 agctgagccc gcacatgatc taagaaaaag tctcatctac cgcaaggcag agagtgaagt
     3361 gcgcgcacag caggcgatgg tgtcgctgtc gcgctcggcg ctttttcccg aagtgaaggt
     3421 gtctgccgcc tacatgttcc gccaaggtgt gccgggcagt agcatggccg aagacatggt
     3481 cgcggtttcg gcttccaccc ctttgccggt cttttatccg ctcaagaaca agcacgaagt
     3541 cgaaggccag gaaaagcgcc tgcaggccgc agagcgcatg ctcgccgaca ccgagcggca
     3601 actcgaagca caggttgccg ccgaaaccgt acggttaaag acacttctcg cctctgctga
     3661 aaactatgcc cgggcgattt tgcccgcgca cagctctgcg caccgttcgc acctcgcgaa
     3721 cattaatctg atgggcggca gcgcggccga agcgctgatt gcttaccgca tgtacctcac
     3781 cgccggtgaa gaaagactgc ggcagatacg tgaggcgcac tcagccctct ttaagatcga
     3841 atacctgcgc gcacagggtg tgcacaacca ggagtccaaa tgaaaaatct ttttaaatca
     3901 atctcgttgt ttcgtttcct gctgctcatc gctctaacgg caattctcac cgactgcgcg
     3961 aagaaaaaag aaccggaagt ctggtactgc cctatgcaca cgcactaccg taccgatcat
     4021 gcgggtaaat gtccgatttg tggtatggct ctcgtaaaag atgaagctcc cactaaagag
     4081 gccagtgcac acgttgcgga acagcacaca aaaaaagaag aagctaaacc ggcaccagct
     4141 gcgacaatca ccgtcagccc tgagcagcaa aagctcgtgg gcatcacgac cgcaaagccg
     4201 caagagcgta aactttcgct cacgctgcag cttgccgggc aggtggcgta tgagcccgat
     4261 atttacgcgg caatcatcga ataccgccag ctcgccgcgg ctgcacacac gctcgaaggt
     4321 tccgtcggtg gcagtgcgaa cctgacgcag agtgcagtgc tgcgcctgcg ccagctcggt
     4381 gtcagcagtg acgagatcgg gcagtacgcg cggtcagaaa ctgcggcctc gcgactcatc
     4441 accggtaacg gcggtggcaa ggcgttgatt acgctcaggc tttcagaagc agacctcgcg
     4501 ctcgtgcgca agggtatggc ggtgcaagtg actacgcctt cgtttccagg caaggtatgg
     4561 aacggccagg tcacggcgat cggcagtctc gtcgacgcga agaaccgcag cctctcggcg
     4621 cgcgtgctcg tttctgaccg cggtcagctc acttcacaga tggctgtgac tgccgaactt
     4681 gctattcgtg cgggcagtgg agtgagcatc gaacgctccg cggtgctgga cacgggcaca
     4741 cgccagatcg tcttcgtgaa agtatcaccc actgaattcg aaccgcgcgc ggtgcgggtg
     4801 ctcggcggca acgacgactt tgcgctggtt tcgggaatca cggcaaagga cgaagttgcg
     4861 atttcctccg cgttcctgct cgattcggaa gcgaagttgc ggttgggtga ttctgtttta
     4921 gtgtccccca cccggcctcc cccgcatgcg ggggaggtgc cataggcgga gggggacatg
     4981 atccgtagaa tcatcacctt ctcggccgag aaccgcgcgc tgacgctctt ccttaccctc
     5041 gtggtgaccc tcggcgcgtg gtatgcaacg aagacaatcc gccttgatgc tctccccgat
     5101 ctttccgata cgcaggtgat cgtctacacg cgctgggaca gaagccctga tgtcgtcgaa
     5161 gcgcaggtca cgacaccgat cgtgcgtgca ctgatgggag ctcccaaggt gaagacgatt
     5221 cgcgccgtaa cggattatgg cacgtcgttc gtctacgtca tcttcgacga tgcgactgac
     5281 ctctattggg cgcgctcgcg catcagcgaa tacatggacc gcattcgcac gctgattcct
     5341 gcagatgcgc ggcttgagct ggggcccgat gcgaccggac tgggttgggt ttaccagtac
     5401 gtgctgcgcg acaagacagg caagaacgac ctcgagcgcc tgcgttcgct gcaggacttt
     5461 accatcaagt ac
//
LOCUS       DAOQJF010000223         5471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8613_length_5471_cov_4.752216, whole genome shotgun sequence.
ACCESSION   DAOQJF010000223 DAOQJF010000000
VERSION     DAOQJF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8613_length_5471_cov_4.752216"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PLF85_11620"
     CDS             complement(<1..1161)
                     /locus_tag="PLF85_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20361.1"
                     /translation="MSGYWQFYPGNLLTGFDLRLSEDTPGTAAIDVIYVKVPSDWNSY
                     EVSGRHFGSHGAGTYTTWIENLEPGELYAIRLNFAGSAHQIFAGDEAQPLCAAGKPAL
                     TAAEYTADYRHVLCRFVASDRRMRLTIQVANFLHAAGGLRDVPIVGKEALVWREYLIH
                     STAAVASLAFLFGVIISLGVGFLLNRTEYRSLYMIGFALVMVIHALNGDIRLGRELFE
                     FYGFLPQAKLNMFILPFGAAMLLLYVVTWLADGRVRRGVRYLALGLVAWSLLFLFSPL
                     TLGVRLYFINLQATAVLAALFVVLMGYALNKGRGEIWILAVGTVFLALGALAAVLRTL
                     HLIELQGLEFIGFMLFLATQTSQSVVELTRIRRRQQTLISENKAALGRLSLFV"
     gene            complement(1351..2652)
                     /locus_tag="PLF85_11625"
     CDS             complement(1351..2652)
                     /locus_tag="PLF85_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE20362.1"
                     /translation="MTSAQITVPVSTVAAAEAVSVVKSGNRIFFHGMGATPHRLIAAL
                     VERASDLRKVEIVHLHTEGEARYAKPEFRDSFFVNNLFVGGNLRTAIDDGRADYIPVF
                     LSEIPTLFRRGVLPLDVAFVSVTPPDAHGFCSLGVSVDVALAAVQSAKVVIAQVNRHM
                     PRTHGDGNIHASRFHYLVEGHDPLPGHEPEPIGEVERQIGEKVASLIEDGSCLQMGIG
                     GIPNAVLASLTGHKRLGIHTEMFSDGVIDLVERGVITCEEKTIHRGRIVAGFVSGSQR
                     LYDFVNDNPFVHLLDITYVNDTSAIRKLKKMVAINSAIEIDLTGQVCADSIGTRQFSG
                     VGGQMDFVRGASLSEGGKPILALPSVTNKGISRITPILKPGAGVVTTRAHVHYVVTEY
                     GIADLYGKNLRQRATELIRIAHPNHREELERAAFERFKRHA"
     gene            2914..4230
                     /locus_tag="PLF85_11630"
     CDS             2914..4230
                     /locus_tag="PLF85_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE20363.1"
                     /translation="MSSVQEMMSQYDQEHLNKTLADFVLQRREKPDLFAWCGPFSESV
                     DYQLSIGHHMYRRVMLSANANRVIVEDQKTGKAKEMIMMGSNSYLGLNAHPKVKAAAI
                     AAAEKYGAGAGSPPHFSGYYDVHRALEQQLSELKGTEDTILYPSGYSTNVGVLSCFLG
                     PKDTIIIDKLAHASIIDGALLSGAKITTFRHNDMDSLERVLIQTKKGLGDRIVVVEGV
                     YSMDGDIAPLKDIYDLVKKHDAKLMVDEAHSTGVLGKTGKGSPEHFDLEGKLDIVMGT
                     FSKSLGGVGGFISAKKEVVRYLRYFSRAYFFAASPTPMQVAAQSAALEVLKTEPKWHE
                     QLWSNIKYFHAELKKRNFDIERTQTAITPIVIGDTFKMREITRFMDEHGVFVNPVPYP
                     AVARKKDRLRLSLSALHTREDLDKVLSLLDEAEAKYKFVKIEEESH"
     gene            complement(4305..4640)
                     /locus_tag="PLF85_11635"
     CDS             complement(4305..4640)
                     /locus_tag="PLF85_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20364.1"
                     /translation="MESSGHDEWSRVSEYLDRLAFENDRLEAELRVKKQRRLEREAAF
                     EGHAPGNQDSRFDEIAANSAYEARNATDATAGMHISYDALRRDLSGLMSDLNDFKSTR
                     AGLEARLGA"
     gene            4706..5182
                     /locus_tag="PLF85_11640"
     CDS             4706..5182
                     /locus_tag="PLF85_11640"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNE20365.1"
                     /translation="MTVRRTLHQVRSWSAATRISATFVAMLFAACNQVQHRQNGLVFG
                     DWYIVKPVVDNGMTTAYGTITNSAAGATTLDAVALDCAEKVELHETIESAGRVSMVGL
                     GRVTLPAGLTLAFQPGKKHLMISGLRQIADGKCRATFTVAGRGISFEIPIRDRRDR"
     gene            5281..>5471
                     /locus_tag="PLF85_11645"
     CDS             5281..>5471
                     /locus_tag="PLF85_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE20366.1"
                     /translation="MPLGLKPDTELTGQNGQRDKLVSHLGQVNLLLFGFTRCPDFCPM
                     TLHRLQSAIGDDAELKAKIA"
BASE COUNT         1313 a         1537 c         1476 g         1145 t
ORIGIN      
        1 cacaaagagg ctaagtcgac cgagtgccgc cttgttttcg ctgataagcg tctgctgtcg
       61 ccgcctgatt cgcgtgagct ccaccactga ctggcttgtc tgcgtcgcga gaaacagcat
      121 aaagccgatg aattcaagac cctgcaattc tatcaggtgc agtgttcgca gcaccgcggc
      181 caaggcccca agagcaagaa acacagtacc cactgccagt atccagattt caccacggcc
      241 tttgttcagc gcatacccca tgaggacaac aaacagcgcc gccagaacag cagttgcctg
      301 caagttaata aaataaagtc tcaccccaag tgtgaggggt gagaaaagga agagtaaaga
      361 ccaggcgacg agccctaatg ccaaataccg cacgccgcgg cgcacacgac cgtctgctag
      421 ccaggtcacc acatagagca acagcatcgc tgccccaaat ggcagaatga acatgttgag
      481 ttttgcctgc ggcaagaagc cgtagaactc aaagagctcc cggccgagac gtatgtcacc
      541 attcagtgca tggataacca taaccagcgc gaagccaatc atgtacaagc tgcggtattc
      601 cgtacggttc aacaagaagc ccacacctag cgagatgatg acaccgaaca gaaatgccag
      661 cgacgctact gcagcggtag aatggatcag gtattcacgc caaacgagcg cctcttttcc
      721 gacaatgggg acgtcacgca gaccgcccgc cgcgtgcaga aaattcgcga cctgtatcgt
      781 caggcgcatg cgcctgtctg aagcgacgaa ccggcagagc acatggcggt agtccgctgt
      841 atactctgcg gcggtaagtg ccggtttgcc cgctgcacac agcggttgcg cttcatcacc
      901 cgcgaatatc tggtgcgcgc tgccggcgaa attcaggcgt atggcataca gctcgcctgg
      961 ctcaaggttt tcgatccacg tggtataggt gccggcgcca tgactgccga aatgccgccc
     1021 agatacctcg taactgttcc agtcagatgg cactttcaca tagatgacat caattgctgc
     1081 agtacctgga gtatcttcgc tgagcctaag gtcaaagcct gtcagcaggt taccggggta
     1141 aaactgccag tagccgctca acgcgctggt ttcctgcgca cttatggcga aggagcttgc
     1201 agatagcggc gcgactgtag caaaagatac aatagcgaga agaagactga aacgccagga
     1261 tcgcatgaac cggtatctgt gagagctgcg aatccgaaga tgattcggtc gtcacgctga
     1321 cgaccgaatt accaagttct ttctgaccga tcaggcatgc cgcttgaaac gctcgaatgc
     1381 tgcgcgctcg agctcctcgc ggtgattcgg gtgcgcaata cgaataagct cagtggcacg
     1441 ctgccgcagg ttcttgccgt agagatctgc gataccgtat tcggtgacaa cgtagtgtac
     1501 gtgcgcccgt gtggttacaa cacccgcacc gggttttaga atcggcgtaa tgcggctgat
     1561 gcccttgttg gttacgctcg gcaaggcgag tatcggcttg ccaccttcgc tgagcgaagc
     1621 accgcggaca aaatccatct ggccgccgac accgctaaac tgccgcgtac cgatcgaatc
     1681 agcacacacc tggcctgtca ggtcgatttc aatagcgctg ttgatggcga ccattttctt
     1741 cagcttgcgg attgcactcg tatcgttgac gtacgtgata tcgagtaagt gcacgaaagg
     1801 attatcgttc acgaagtcgt agaggcgctg cgaaccgctg acaaaccccg caacaatgcg
     1861 cccgcggtgt atcgtcttct cttcgcaggt aataacaccg cgttccacga ggtcgataac
     1921 tccatcgctg aacatctctg tatgaatacc gagccgcttg tgccccgtga ggctcgcgag
     1981 caccgcgttg ggaattcccc cgatacccat ctgcaggcat gagccgtctt cgatgagcga
     2041 ggccactttc tcgccgatct gccgctcgac ttcgccaatg ggttcaggct cgtgccccgg
     2101 caggggatca tgtccttcga ccaggtaatg aaacctgctg gcgtgtatgt tgccatcgcc
     2161 gtgggtgcgc ggcatgtgcc ggttgacctg tgcgatgact accttcgccg actgcactgc
     2221 cgcgagtgcg acatcgacac tgacaccgag cgagcagaac ccgtgcgcat cgggtggcgt
     2281 gactgaaacg aacgctacat caagcggcaa cacaccgcga cgaaacagcg tgggaatctc
     2341 gctcaagaat acgggaatgt agtcagcgcg cccatcatca atcgctgttc tcaggttacc
     2401 gcccacaaac aggttattca caaagaagct atcgcgaaat tccggctttg cgtagcgtgc
     2461 ctcgccctcg gtatgcaggt gcacaatttc gaccttgcgc agatcactcg cccgctccac
     2521 cagtgcggca atgagccgat gcggtgtggc gcccatgcca tggaaaaaaa tgcggttacc
     2581 cgatttgacc acgctgacag cctcagcggc tgcaacggta cttacgggga ctgtgatttg
     2641 tgcactcgtc atttgagctc ctcaatagtt atgcgagtaa ctgcgataac tgtgttcaaa
     2701 aagcgaggca aatggcgatg taaagcgtgt ttcagcgctc tttttcgcag ctgcagcaaa
     2761 gagaaaacct gaatcataga tttttggcac ggtgtcgaag gttgggcaat aaggttgacg
     2821 cattgtgcgc cgcctaaaaa atgactaaaa tccaaagtta aacgtctaaa ttgtatcaat
     2881 gcgaattata gaaagaggca ttgaaggagc aacatgagtt cagtacaaga aatgatgtca
     2941 caatacgatc aggagcacct gaataagacg ctcgctgatt tcgttctgca gcgccgtgaa
     3001 aagccagatc tttttgcgtg gtgcggaccc ttttctgaat cggtagacta ccagctttct
     3061 atcggtcacc acatgtaccg ccgcgtaatg ctgtcggcca acgccaaccg cgtgatcgtc
     3121 gaagaccaga agaccggcaa agctaaagaa atgatcatga tggggtcgaa ctcttacctc
     3181 ggcctcaacg cgcacccgaa agttaaagcg gcagcgatcg ctgcagcaga aaaatacggc
     3241 gctggcgctg gttcgccccc gcacttctcc ggttactacg acgtgcaccg tgcactcgag
     3301 caacaactgt cggagctcaa gggtactgaa gacacgatcc tgtatccttc cggctactct
     3361 actaacgtcg gcgtgctctc gtgcttcctg ggcccgaaag acacgatcat catcgataaa
     3421 ctggcgcacg catcgatcat cgacggcgcg ctgctctcgg gcgcgaagat cactacattc
     3481 cgccacaacg acatggattc actcgagcgc gtgctgatac agaccaagaa aggtcttggc
     3541 gaccgcatcg tcgtcgttga gggagtttac tcgatggacg gcgatatcgc cccactcaaa
     3601 gacatctacg acctcgtgaa gaagcacgac gcgaaactga tggtcgacga agcacactca
     3661 acaggtgtac tcggtaagac cggcaaaggt tcgcctgaac acttcgacct cgaaggtaaa
     3721 ctcgacatcg tgatgggcac attctcgaaa tcgctcggcg gtgtcggtgg ctttatctct
     3781 gcgaaaaaag aagtggttcg ctacctgcgc tacttctcac gcgcgtattt cttcgctgcg
     3841 tcacctaccc cgatgcaggt cgcagcccag tcagctgcgc tcgaagttct gaagactgag
     3901 cccaagtggc acgaacagct gtggagcaat atcaagtact tccacgcaga actcaagaag
     3961 cgcaactttg acatcgaacg tacgcagaca gcaatcaccc cgatcgtgat cggcgacaca
     4021 ttcaagatgc gcgaaatcac gcgcttcatg gacgaacacg gcgtattcgt caacccggta
     4081 ccataccccg cggttgcacg caagaaagac cgcctgcgcc tgtctctgtc tgcgctgcat
     4141 acgcgcgaag acctcgacaa ggtgctgtcg ctgctcgacg aagcagaggc gaagtacaag
     4201 ttcgttaaga tagaagaaga aagtcactga cgcttcgact gcgctcagcg atcagtgagc
     4261 ggagtcgaac tgatcagctg aaaaggcctc tatggggcct ttttttatgc gcccagccgg
     4321 gcttcgaggc ccgcgcgggt cgatttgaag tcgttgaggt ctgacatgag gcctgaaaga
     4381 tcgcggcgca gagcgtcgta cgagatgtgc atacccgcag tggcgtcggt cgcgttgcgc
     4441 gcttcgtaag ctgagttggc agcgatctcg tcaaaacggg agtcctggtt accaggcgca
     4501 tgcccttcga acgcagcttc gcgttcgagc cggcgctgct tttttacgcg cagttctgcc
     4561 tcgagacgat cgttctcaaa tgccaggcgg tcgaggtatt cactgacccg cgaccattcg
     4621 tcgtgacctg agctttccat actatatctt cggtttctgg gtaaaaaagc ttgatggcgg
     4681 gtcttgcggc aggggtacga aagctatgac cgtccgccgc actctgcacc aggttagaag
     4741 ctggtcggct gctacgagaa tttcggccac gttcgtcgca atgctttttg ccgcctgcaa
     4801 ccaggtgcag caccgtcaaa atggtctagt ttttggcgat tggtacattg tgaagcccgt
     4861 agtggataac gggatgacga ccgcatacgg cacgatcaca aattccgccg ccggagccac
     4921 gacgttagac gctgttgcac tcgattgcgc ggagaaagtc gaactgcatg aaaccatcga
     4981 atctgccggt cgcgtctcga tggtggggtt aggtagggtc acgcttccgg ccggcttgac
     5041 cttggccttt cagcccggca aaaagcacct gatgatcagt gggttaagac aaattgccga
     5101 tggaaaatgc agggcaacgt ttacggtggc tggccgaggt atcagctttg aaattcccat
     5161 acgggacaga agggacagat gatcaactca ccctattttt cctcaaggaa cccggtattc
     5221 tcagctctgt tactcggtgt tctactgtca ggctgctctg accgcataac cttccgcgaa
     5281 atgccgctgg gactaaaacc cgacacggag ttaaccggcc agaatgggca acgcgataaa
     5341 ctggttagcc atcttggcca ggtcaacctg ctgttattcg gcttcacacg ctgccccgat
     5401 ttttgcccga tgacgttgca ccgcctgcag tctgccattg gcgacgatgc cgaactcaag
     5461 gcaaaaattg c
//
LOCUS       DAOQJF010000224         5460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8636_length_5460_cov_4.793710, whole genome shotgun sequence.
ACCESSION   DAOQJF010000224 DAOQJF010000000
VERSION     DAOQJF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8636_length_5460_cov_4.793710"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..572)
                     /locus_tag="PLF85_11650"
     CDS             complement(33..572)
                     /locus_tag="PLF85_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE20367.1"
                     /translation="MASLITHPVVPLSLGIAFGGQWIPRFAVITGIVFSLLPDADVIA
                     FRFGIPYGHMLGHRGASHSIMFAMLLASAAVALPAFNGMRLRIFGFLFISALSHGILD
                     AMTTGGLGVGFLIPFSPERYFLPLRPIRVSPLSVDGFFTQRGLQIFVSELLAVWLPCG
                     IFALTAWLIRRWSGRGENS"
     gene            complement(573..1034)
                     /locus_tag="PLF85_11655"
     CDS             complement(573..1034)
                     /locus_tag="PLF85_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE20368.1"
                     /translation="MSRPFKILGVQQIAVGGSDKAKLRRLWVDVLGLTPKSEFRSERE
                     NVDEDILEMGHGVHAVEVDIMQPLDPAKKPRVDEPALNHIGLWVDDLAVAVEYLTRQG
                     MRFTPGGIRKGAAGYDVCFIHPKGNEEFPLCGEGVLIELIQAPDHIIAALD"
     gene            complement(1110..1394)
                     /locus_tag="PLF85_11660"
     CDS             complement(1110..1394)
                     /locus_tag="PLF85_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20369.1"
                     /translation="MREKVYCANCEHCHVVRVFEADATKYVLRVKCAKKRWAKRSGEE
                     KQYKYFTVARRVVSDCPDYSPMGDPNPFIKNLRRELPVKDEIYTLKESEA"
     gene            1513..2016
                     /locus_tag="PLF85_11665"
     CDS             1513..2016
                     /locus_tag="PLF85_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNE20370.1"
                     /translation="MLKTLEADELKILNALAENGLLGNQELASLVKLDEKRVGEIRSE
                     LESAGIILKYRAIINWEKIESGGVNAVIQVKVTPAQGQGYDEVARRVAAFPEVRTCLL
                     VSGSFDLLVEVDGPSLRDVAFFVADKLATIEGVNHTSTNFLLKRYKQSGDMFSASGKT
                     HRLPMVI"
     gene            complement(2063..2770)
                     /locus_tag="PLF85_11670"
     CDS             complement(2063..2770)
                     /locus_tag="PLF85_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20371.1"
                     /translation="MLQRFLILGLLAVLATACREKHEPRARLDIPLNQKIDALLKEAQ
                     AATDDNRKAALFGEASELLIEKGDLRQAMVAARQGERANPTQRQCLTSIAEIQLSEGK
                     IAEAATTLADTLQRHPKYGRAHYVHGNLLASKTDYKAALQSYAKAEQNGFAEQRLYLN
                     SAGVALRGKKAAEALATYERALKKFPNLAEASLGAGIAAGQLNKKKEARKYFETYLTL
                     APNSSEAGRVRSWLKKL"
     gene            complement(2796..3398)
                     /locus_tag="PLF85_11675"
     CDS             complement(2796..3398)
                     /locus_tag="PLF85_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE20372.1"
                     /translation="MRQEKRKELTREKILNAARAVFAQKGYGGTSIADIVKKSKLARG
                     TFYLHFQSVEQVLNALLQEIFIDIQRYLTDMQVEALDQKNFKIALSDLAKSLLSVFQG
                     HRETVILLLTTMNAEPKIRAQTIWFQELMQASIRVMLDRGITSGRLKKHDSELMSFLV
                     SGGLREVLSQWLLFGRYEKQIESKVDEMIAIYMNGVELSN"
     gene            3504..3959
                     /locus_tag="PLF85_11680"
     CDS             3504..3959
                     /locus_tag="PLF85_11680"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNE20373.1"
                     /translation="MLDEATLRTALSAAYAEAELAFQENEVPVGAVIVYNNEIIARDH
                     NRIVQHANPVRHAELSVIEKAALVLKNERLVDCHLVSTLEPCMMCSGAVVHARIASVH
                     FLADEERSPGLRQLLVLPSVNHKPQLYRHRSEEWDAPSLMRRFFQLRRT"
     gene            3956..5065
                     /gene="alr"
                     /locus_tag="PLF85_11685"
     CDS             3956..5065
                     /gene="alr"
                     /locus_tag="PLF85_11685"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNE20374.1"
                     /translation="MNQFPETAPYPELRIHRAALAHNLSVLRRLAPRSRVLLPVKANA
                     YGCGMETLFPFFREAEVDMLGVANCNEAVQLRRLGWKKPVLNLGGFFAQNLGAIVAHE
                     ITPSITDLWQVAALDAAAAGRTIEVHLKLDLGMGRIGIRPEDFPELLRLLAEAKALRV
                     AGIFTHFPHAGPAAREKTLAQNESFQQHVTAILSALRLERGSVILHAANSYSAAFFPE
                     THHDMIRPGILFYGYYQSEPDRVEFNPRFSFKPSLELVVTPISRRILRRGDTVSYSGL
                     YTVTDEREQIAVLPLGYADGIPRALSNQVSFGRYPLRGRVTMDQIMLGNYDGVSPVRL
                     LGEGVTSLEQWGELSLSFSYEIMSHLGNRLNRVLV"
     gene            5175..>5460
                     /locus_tag="PLF85_11690"
     CDS             5175..>5460
                     /locus_tag="PLF85_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20375.1"
                     /translation="MKKILLLAVASVIAVTACKKSVSGQTGQWTSNLNELAEASGKYP
                     NLKALLDAKATEAKAIFAEAEKISDEEQKAEKIAAAIAKLKENLGVVVEVK"
BASE COUNT         1185 a         1490 c         1534 g         1251 t
ORIGIN      
        1 aattctgccg ggaaaaaaga cattcgtttc actcaggagt tttcgccgcg gcctgaccag
       61 cggcgaatca accacgcagt aagcgcgaaa atcccgcagg gcagccagac agcaagaagt
      121 tcagatacga atatctgcag gccgcgttgg gtaaaaaaac cgtccacact aagcgggctg
      181 acgcgaatcg ggcgtagcgg aagaaagtaa cgctcggggc taaacgggat caggaaaccg
      241 acgcccaggc cacccgttgt cattgcatcg agaatgccgt gcgacagcgc gctgataaac
      301 agaaagccaa aaattctcag gcgcatgccg ttgaatgcag gcaaggcaac ggcggccgac
      361 gccagaagca tcgcaaacat gatggaatgc gaagcgccac ggtggcctag catgtgaccg
      421 tacggaatgc cgaagcgaaa tgcaatcacg tcagcatcgg gcagcaggga aaatacgatg
      481 ccggttatga ccgcgaatct tggtatccac tggccgccaa aggctatgcc aagcgacagg
      541 ggaacaacgg gatgagtaat taaggacgcc actcagtcga gggctgcaat gatatggtcg
      601 ggtgcctgga tcaattcaat gaggacacct tcaccgcaga gagggaattc ttcattgcct
      661 ttcgggtgaa taaaacagac gtcataaccc gccgcaccct tgcgaatgcc gccgggcgtg
      721 aaccgcatgc cctggcgcgt caggtattcc accgcaacgg ccaaatcatc gacccacaga
      781 ccgatatggt tcagggccgg ttcatcgacg cgcggctttt ttgccgggtc gagcggctgc
      841 atgatgtcga cttcaacagc gtgcacgccg tgtcccattt cgaggatatc ttcgtcgacg
      901 ttttcgcgct cgctgcgaaa ttctgattta ggcgtgagcc ccagcacgtc tacccacagg
      961 cggcgcagct ttgccttgtc ggaaccaccg accgcgatct gttgaactcc cagtattttg
     1021 aaggggcgtg acataatatt gtgctaaagg tcacttcggc tgcgctcagt gacctttaga
     1081 ccggtcggtg agcgcagtcg aaccgacggt taggcttctg attctttcag cgtataaatt
     1141 tcgtctttaa ccggcagctc gcggcgcagg ttcttgatga acgggtttgg atcgcccatt
     1201 ggagaataat cggggcagtc agaaacaacc cggcgcgcca cggtgaagta cttgtactgt
     1261 ttttcttcgc ctgagcgttt agcccagcgc ttctttgcgc attttacccg cagaacgtat
     1321 ttggtcgcat ctgcttcaaa gacgcgcact acatggcagt gttcgcagtt cgcgcaatag
     1381 actttttctc tcattctatt ggcttttcgg ggcggggaga catggggtca atgaaaatcg
     1441 accccaaaac cagccgcatc ggccaaaaac gtgaatcgat tgaccccgcg tttcagcggt
     1501 gcgcgcttac gcatgctcaa gacgctcgaa gctgacgaac tgaagatcct gaatgccctc
     1561 gccgaaaacg gccttctggg taaccaggag ctggcctcgc tggtgaaact cgatgaaaaa
     1621 cgggtggggg agatcaggtc tgagctcgaa tcggccggca ttatcctcaa gtaccgtgcc
     1681 attatcaact gggaaaaaat cgaaagcggc ggagtgaacg cagtcatcca ggtcaaagtc
     1741 accccagccc aggggcaggg ttacgatgag gtggcgcggc gcgtggcggc ttttcccgaa
     1801 gtgcgcacct gcctactcgt ctctggttcc tttgatctgc ttgtcgaggt cgacggccct
     1861 tcgctgcgcg acgtggcctt tttcgtcgcg gacaagctgg cgacgatcga aggggtaaac
     1921 cacaccagca cgaacttttt gctcaagcgc tacaagcagt ccggtgacat gttctcggcg
     1981 agcggtaaaa cccatcgttt gccgatggtg atctgagcac gccgggcgcc cgaaaaaaat
     2041 tgaggacagc cgtcttcggc gcttacagct tctttaacca cgacctgacg cgcccggctt
     2101 ccgatgagtt cggtgccagt gtcaggtagg tttcgaaata tttcctggcc tctttctttt
     2161 tgttgagttg cccggccgct attccggccc cgagagaggc ttcggcaagg ttcgggaatt
     2221 tcttcagtgc gcgttcatat gtggcgaggg cctctgcggc ttttttgccg cgcagggcga
     2281 ctcctgcaga gttgaggtat agccgctgct cggcaaaacc attttgctcg gctttggcgt
     2341 agctttgcag ggcagccttg tagtcggtct tactggccag aaggttgccg tgtacgtagt
     2401 gcgcgcggcc atacttgggg tgccgttgca gcgtgtcagc cagcgtcgtc gccgcctcgg
     2461 caatcttacc ttcggacaac tggatttctg cgatcgacgt gaggcattgt ctctgcgtcg
     2521 ggttggcgcg ttcaccctgc cgtgcggcta ccattgcctg cctcagatca cccttttcaa
     2581 tcaggagctc cgacgcttcg ccaaatagtg ccgccttgcg gttgtcgtcg gtcgctgcct
     2641 gcgcctcttt cagaagtgca tcgatctttt ggttcagcgg gatgtcgagc cgtgcacgcg
     2701 gttcatgctt ttcacggcac gcagtggcca gaacggccag taatcccagt atcaagaatc
     2761 tttgtaacat ggaaagctcc cccgatgagc aaatcttaat tgctcagttc aaccccattc
     2821 atgtagatcg caatcatttc atcgactttg ctctcgattt gtttttcgta gcgtccgaag
     2881 agcagccact gcgacagcac ttcacgcagg ccgccggaaa ccaaaaagct catgagttct
     2941 gagtcatgtt tcttgagcct tcctgaagta ataccgcggt cgagcatcac cctgatggac
     3001 gcctgcatca gctcctggaa ccagatggtc tgtgcccgta tcttgggctc tgcattcatg
     3061 gtcgtgagca gcaggatgac ggtttcgcgg tggccctgga aaaccgacag cagtgatttt
     3121 gccaggtcgc tcagggcaat cttgaagttc ttttggtcga gcgcctcgac ctgcatgtcg
     3181 gtaaggtacc gctggatatc gatgaaaatc tcttgcagca gggcgttgag cacctgttcg
     3241 acgctctgga agtgcaggta gaaggtgccg cgcgccagct tgctcttctt tacaatatcc
     3301 gcgatgctgg tgccgccata acccttctgg gcaaatacgg cgcgcgccgc attgagtatt
     3361 ttttcgcggg taagttcttt ccgtttttcc tggcgcattt tcgtagcttt agtcaacgtg
     3421 ggctccctgt ggagagagaa cctcgttgga caacctgtct cgaaaagcgg catcggtttg
     3481 ccagtagaat ttaggccgtc atcatgttag acgaagcaac actgcgcact gcgctctccg
     3541 ctgcctatgc cgaggccgaa cttgccttcc aggaaaacga agtgccggtg ggggctgtta
     3601 ttgtctataa caacgaaatt atcgcgcggg accacaaccg tattgtacag catgcaaacc
     3661 ccgtgcggca tgctgaactc agcgtcatag agaaggcggc actggtgctc aagaacgaac
     3721 gcctggtcga ctgccatctc gtcagtacgc tcgaaccctg catgatgtgt tccggcgcag
     3781 tggtgcatgc ccgaattgcc tcggtgcact ttctggccga tgaagaacgc tcccctggtc
     3841 ttcggcagct gcttgtatta ccttcagtca atcataagcc acaactctat cgtcaccgca
     3901 gcgaagagtg ggatgcgccg tcgctgatgc ggcgcttttt ccagctgcgc cgtacatgaa
     3961 ccagtttcca gagacagctc cgtaccccga actgcgcata caccgcgcag cactggcgca
     4021 caatctttca gtattacgcc gccttgcgcc acgcagtcgt gtgctgctgc cggttaaggc
     4081 gaatgcctat ggctgcggta tggaaacgct ttttcctttc tttcgcgaag cagaggttga
     4141 tatgctcggt gtcgccaact gcaacgaagc agtccagctg cgccgcctcg gttggaaaaa
     4201 gcctgtcctg aacctcggtg gtttctttgc gcagaacctc ggcgcgatcg tcgcgcacga
     4261 aattaccccc tcgataaccg atctctggca ggttgctgcg cttgatgccg ctgccgcggg
     4321 tcgcaccatc gaagttcatt tgaagctcga tctcggcatg gggcgtatcg gcatacgccc
     4381 cgaagatttt cccgaactgt tgcgattgct ggcggaagct aaagcgctgc gggtcgccgg
     4441 tatcttcacc cattttccac atgcgggtcc ggctgcgcgt gagaagacgc tggcacaaaa
     4501 cgaaagtttt cagcagcacg ttacggcgat tctttcagcg ttgcggctcg aaagggggag
     4561 tgtcatcctg cacgcggcaa actcctattc ggcggctttt ttcccggaga cgcatcacga
     4621 tatgatccga ccaggaattc ttttttatgg gtattaccag agcgaacctg accgtgtaga
     4681 gttcaacccg cgcttttctt ttaaaccatc actcgagctc gtggtcacac cgattagcag
     4741 gcggatactc cgccgcggcg acacggtttc gtattcggga ctttataccg taaccgacga
     4801 gcgcgaacag atcgcggttt tgccgcttgg gtatgccgac ggtatcccgc gcgctctttc
     4861 gaaccaggta tctttcggca ggtacccgct gcgtggacgc gtgaccatgg accagatcat
     4921 gctgggcaat tatgatgggg tgtcgccggt ccgattgttg ggtgaaggtg ttacttcact
     4981 cgaacaatgg ggcgaactct ctttgagctt ctcgtacgaa atcatgtcac acctgggaaa
     5041 ccggctgaat cgtgtccttg tgtagcggtt cgcttgaggt aaagtggtca aaggattggg
     5101 gacgtcagcc aaattcggtg gacggtatcg ggcaggtctt tacactgtgc ggacaaaaaa
     5161 tctccggagg aaacatgaaa aaaattcttt tattggccgt tgcgtcggtc attgccgtca
     5221 cggcctgtaa aaaaagtgtg tcaggccaga ctggccaatg gacgagcaat ctgaacgaac
     5281 ttgccgaagc ttcgggcaaa tatccaaacc ttaaggccct gctcgatgcc aaggcgactg
     5341 aggctaaggc aatttttgcc gaggcggaaa aaatcagcga tgaagagcag aaggcagaaa
     5401 aaattgcagc ggcgattgcg aagctgaaag aaaacctcgg cgtcgtcgta gaggtaaaat
//
LOCUS       DAOQJF010000225         5458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8641_length_5458_cov_5.147140, whole genome shotgun sequence.
ACCESSION   DAOQJF010000225 DAOQJF010000000
VERSION     DAOQJF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8641_length_5458_cov_5.147140"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLF85_11695"
     CDS             <1..509
                     /locus_tag="PLF85_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNE20376.1"
                     /translation="LYGAQLAAAYGLPYAFASHFAPHQLFDALRQYRQYFRPSQYLAK
                     PYVMLGFNVIAAESDEEAELLATSGQQAFVNLRTGNPTPLPPPVRNFKQSLEPMLRTM
                     LDQILSCSAVGSAQTIRTRLAEFMEETQADELMIASHIFDHTARLKSYEILAQVAAGL
                     KPAAAVSA"
     gene            complement(499..1476)
                     /locus_tag="PLF85_11700"
     CDS             complement(499..1476)
                     /locus_tag="PLF85_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNE20377.1"
                     /translation="MAQPATTPVHIADVLKSNKPALSFEFFPPKTPEGSETLFQTISE
                     LKPLEPAYVSVTYGAGGSTRQLTHDLVVRLSRELGLTIVSHLTCVGASKDEIRQILER
                     YQESGISNILALRGDPPVSKDASGVGAAKDGTPGAAPKEAFARFVPHPDGFHYAAELV
                     AFIKKHFPQMGVGVAGFPEGHPETPNRLKEIEHLKAKVDAGADYICTQLFFDNHDFYD
                     YRERCELAGIHVPIIAGIMPITSRKGMQRMAELSLGSRFPAKLLRALARAEDDEYAEN
                     VGIHWATEQVRDLIDHGVAGVHFYTLNKSKATLKIYQSLGIRKSTALKR"
     gene            complement(1524..1973)
                     /locus_tag="PLF85_11705"
     CDS             complement(1524..1973)
                     /locus_tag="PLF85_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20378.1"
                     /translation="MSLAEKTAITSAVFLFIVGLVTGIWKYAAIVRSDKAAAPVYVDV
                     LHRATLMYSFACMILYALAGLSVYPDSVNTAAVLSVVVFFVFANGTYLVHALLKDTDN
                     QFEKPYRIGSWQMPKVLFHGSMFALIAGELGGTLVLAVGALTRVWGA"
     gene            complement(2078..2794)
                     /locus_tag="PLF85_11710"
     CDS             complement(2078..2794)
                     /locus_tag="PLF85_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE20379.1"
                     /translation="MPAPQFARLVEHYWSVSWNLEGREPFESETLPHPSVHLVLETQR
                     SEIVGVMRGRFTRRLEGIGFVFGIKFLPGAFHALGRAPVTSFTDRRVAPVKVFGENWH
                     AFEAELLSQQSFDAMAHTTENFLASINPTIDANAELAREIVFTIRDDRSILRVDAVAD
                     EFALSKRQLQRLFAEYVGVSPKWVIERYRMHEAVEQLSAGQECDIADLAHSLGYFDQA
                     HFHKAFRRLTGKSPGEYATR"
     gene            2929..3357
                     /locus_tag="PLF85_11715"
     CDS             2929..3357
                     /locus_tag="PLF85_11715"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE20380.1"
                     /translation="MQNNLTATVHVDIHSPAPAVWDTLTNPDKIAQWLFGTKTTCDWK
                     KGSPITYTGEWQGKQYIDKGTILEIEPHKLLVMDYWSSFSGQPDTPENYQKITYRLDT
                     IAQGTRLTVIQENHRSEETRAQSEGHWQQALVLLKRVSES"
     gene            3404..5440
                     /locus_tag="PLF85_11720"
     CDS             3404..5440
                     /locus_tag="PLF85_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20381.1"
                     /translation="MQRFFLFVSLLSGATATAAAGKKIGSTKVHFVAELRGNDMHMTE
                     EHALDLTEAWQYPSRTYYRSFLAGRFPWSQDLTDFHASVDGQPMKTDLYRYGYDKVML
                     RLPALAAGNYRLKISYTIRDIRGFPEATLPELRFWDYPLHIEEVTAQLTGLPRGAEFA
                     LIERYNREQFAQKRADGIQALAYGKDHDNTPSPVLLISSSALRYDEGIVGYMRYFAQQ
                     SRLLPFVGFLIILMLLLRFMPSRIVLGFISLYMFIPLAFWLSRIPGWLSYFAARKEIE
                     PRAEYIEHFYGELAGDIAVAAILFLLALRVVRGIWRGEISDYYLPYGGLVSFLVLLLF
                     MGQDPEALLMLPLAALFPLMYSGKQVTRFFGADAHLWIEKVISVGKMSFDELSLASGL
                     AVTRLNRIFAALPTHPVAIDHDKRQYLSADALAALEQNQFCNNCGGTLKVEHSDLLQC
                     PYCRSDYAVALGKKSKAKNTPPLVVSTLANFLKASGMMLMIVFVFLAAFGAITELYAR
                     LHRKGGTGGDWAVVITIAILGGAAYGGFAALATRLLSGKGYALVSAVLATLAPLVWPI
                     FALRALMSPRSKFFFGQLDLARIREWLSEKKYIPLIEFARRLRASELDAMNLAHYLTS
                     SGELDAVYDRRNNTLTERSLFRAKAGANSCQSCGGVVSVMNGARVCRYCGTKAT"
BASE COUNT         1113 a         1576 c         1574 g         1195 t
ORIGIN      
        1 gcctgtacgg tgcccagctc gcggcggcgt atggcctgcc gtatgctttt gcctcgcact
       61 tcgccccgca ccagctgttc gacgctttgc ggcagtaccg gcagtatttc aggccctcgc
      121 agtacctggc gaaaccttat gtcatgctgg gcttcaacgt gattgcggcc gaaagcgatg
      181 aagaggcgga gcttttggcg acatccggac agcaggcgtt tgtgaatctg cgcacaggta
      241 acccgacgcc gctgccaccg ccggtgcgga attttaagca gtcgctcgaa ccgatgctgc
      301 gcacgatgct cgaccagatt ttgtcgtgct ctgccgtcgg gtcagcgcag acaatccgca
      361 cccgccttgc ggagttcatg gaagagacgc aggccgatga gctgatgatt gcgtcgcata
      421 tattcgacca taccgcgcgc ctgaagtcgt acgaaattct tgcgcaggtg gctgctggtc
      481 ttaagccagc ggcggccgtc agcgcttgag cgcggtcgat ttgcgaatac ccagcgactg
      541 gtagatcttg agcgtcgcct tcgacttgtt gagggtgtaa aagtgcacgc cggcaacgcc
      601 gtggtcgatg aggtcgcgca cctgttcggt cgcccagtgg atgccgacgt tctctgcgta
      661 ctcgtcgtct tctgcgcgtg caagcgcgcg cagaagtttc gccggaaacc tcgaaccgag
      721 cgaaagctcc gccatgcgct gcatgccctt gcgcgaggtg atgggcatga tgcccgcgat
      781 aatcggcacg tggatgcccg cgagttcgca gcgttcgcgg tagtcgtaga agtcgtggtt
      841 gtcgaagaag agctgcgtgc agatgtagtc ggcgccggcg tcgaccttcg ccttgaggtg
      901 ttcgatctct ttcaggcggt tgggggtttc ggggtggcct tcgggaaaac ctgcaacgcc
      961 gacgcccatc tgcggaaaat gtttcttgat gaatgcgacg agttcagccg cgtagtggaa
     1021 gccatcgggg tgtggtacga atcttgcgaa tgcctctttc ggcgcggcgc ctggagtgcc
     1081 atccttggca gcgccgacgc ccgaagcatc cttgctcacg ggagggtcgc cacgcaatgc
     1141 cagtatgttg ctgatgccgc tttcctggta gcgctccaaa atctggcgga tttcatcttt
     1201 actggcgccc acgcacgtga ggtgagagac aatcgtgaga ccgagctcgc ggcttaaacg
     1261 cactacgagg tcatgcgtca gctgccgtgt cgaaccgccc gcgccgtagg tgaccgatac
     1321 gtacgctggt tcgagcggct tcagctcgct gatcgtctgg aacagcgtct cagaaccttc
     1381 gggggtcttg ggcggaaaaa attcaaaact cagcgcgggt ttgttcgact tgaggacatc
     1441 cgcgatgtgc acaggcgtag ttgccggttg ggccatcgtt ccgggtgggc atcgcttatg
     1501 gcgcgtaaag gagaatcagt cagctatgct ccccatacgc gcgttagtgc ccctaccgcc
     1561 agcacgagag ttccaccgag ctcgccggcg atcagcgcga acatcgaacc gtggaacaat
     1621 actttcggca tttgccagct accgatgcgg taaggttttt caaactggtt gtcggtgtct
     1681 ttcagcagcg cgtgcaccag gtaggtcccg ttcgcgaaaa cgaagaagac gactacgctg
     1741 aggactgctg ccgtgttgac ggagtcaggg tagacactca ggcccgcaag ggcatataaa
     1801 atcatgcacg cgaatgaata cattagggtc gcgcggtgca gtacatcaac atacaccggt
     1861 gccgcagctt tgtcgctgcg cacgatcgcc gcgtacttcc agatgcccgt taccaggcca
     1921 acgatgaaca agaacaccgc tgaagtaatc gctgtttttt cggctaaact cataatttcc
     1981 cctctttgct tctctttaga aaaagaccgc gcagattata tagactggtc tatctgcaaa
     2041 ggtgtttttt ctggtatcca tggcccgggc acaggcttta acgtgtggcg tactcgccgg
     2101 gcgatttgcc cgtcaggcgg cgaaatgcct tgtgaaagtg cgcctggtca aaatagccga
     2161 gcgagtgcgc gagatcggca atgtcacact cttgccccgc cgacagctgc tcgacagctt
     2221 cgtgcatgcg gtagcgttcg atcacccact tggggcttac accgacgtat tcggcaaaga
     2281 gccgctgcag ctggcgtttg ctgagcgcaa attcatcagc gacagcgtcg acccgcagta
     2341 tgctccggtc gtcgcgaatc gtgaagacaa tctcgcgcgc gagctcagca ttcgcatcga
     2401 tagtgggatt gatactggcg agaaaatttt ctgtcgtgtg cgccatggca tcgaaactct
     2461 gctgcgaaag taactccgct tcaaaggcat gccagttttc accgaatact ttcaccgggg
     2521 caacacggcg gtcagtgaaa ctggtgacgg gcgcgcggcc gagcgcatga aatgccccgg
     2581 gcaggaattt gataccgaaa acgaaaccga tgccttcgag acggcgcgtg aagcggccac
     2641 gcatgacacc gacaatttca gaccgctgcg tctcgagcac caggtgtacc gacgggtgcg
     2701 gcagcgtctc tgattcgaac ggttctctgc cctcgagatt ccagctgacc gaccagtaat
     2761 gctcaaccag tcgcgcaaat tgcggcgcag gcaaagcgcg gtgcagctga aaaggctcat
     2821 gcccgtcctg ataagttaaa atcccccttg gctttggttc gacgatatgg gccactgtcg
     2881 cgtttttaca aggcgttgca gccgtgaaaa ccgatattct ggtgtactat gcagaacaac
     2941 ctcaccgcaa ccgtgcacgt cgacattcac tctccggcgc ccgccgtctg ggacacgctt
     3001 accaaccctg acaagattgc gcagtggctc ttcggcacga agaccacctg cgactggaaa
     3061 aagggcagcc cgatcaccta taccggcgaa tggcagggca agcagtacat cgacaaaggc
     3121 acgattctcg aaatagaacc acacaagctt ctggtcatgg attactggag cagcttttcg
     3181 gggcagcccg atactcccga aaactaccag aagatcacct accggctcga caccatcgcg
     3241 caggggactc ggctcaccgt gatccaggaa aaccaccgca gcgaagagac gcgcgcgcag
     3301 tcagagggcc actggcaaca ggcactcgtc ctgctgaagc gggtcagcga gagctgacag
     3361 aaaatagcgt tggcgacgcc tgcccggttt tgcaggcagt cccatgcagc ggtttttttt
     3421 gttcgtttca ttgctctcag gcgccacagc gactgcggcc gccgggaaaa aaatcggcag
     3481 tacaaaggtg cacttcgtgg ctgagctcag gggcaacgac atgcacatga ccgaagagca
     3541 cgcactcgat ctcaccgagg cctggcagta cccatcacgc acttactacc gcagtttcct
     3601 ggcggggcgt ttcccgtggt cgcaggacct caccgatttt cacgcgagcg tcgatggcca
     3661 gccaatgaaa actgatctct accggtacgg ctacgacaag gtcatgctgc gcctgccagc
     3721 tctcgcggcg ggcaactacc gcctcaagat cagttataca atccgcgaca ttcgcggttt
     3781 tcccgaggca actctgcccg agctgcgctt ctgggattac cctctgcata ttgaagaagt
     3841 cactgctcag ctcactggct tgccgcgggg cgcagagttc gcgctgatcg aacgctacaa
     3901 ccgcgagcag tttgcgcaga aacgcgccga tggcattcag gcgctggcgt acggtaaaga
     3961 tcacgacaac acgccatcac cggttttgct catcagcagc agcgcgctgc gctacgatga
     4021 gggcatcgtc ggttacatgc gctatttcgc gcagcagagc agattactgc catttgtagg
     4081 atttctgatt attctgatgc tcttgctcag gttcatgccg tcgcgcattg ttctaggttt
     4141 tatttcgctc tacatgttta taccgctcgc cttctggctc tcgcgcattc cggggtggct
     4201 aagctacttt gccgcacgca aagaaattga accgcgcgca gagtacatcg aacacttcta
     4261 cggcgagctg gcgggcgaca ttgctgtcgc agccatcttg ttcttgcttg cgctgcgtgt
     4321 cgtgcgggga atctggcgcg gcgaaatcag cgattattac ctgccttacg gcggcctcgt
     4381 gagctttttg gtgctactgc tcttcatggg gcaggacccc gaggccttgc tgatgctgcc
     4441 gctcgcagca ctttttccgc tgatgtattc aggcaaacag gtcacgcgtt ttttcggtgc
     4501 cgatgcgcat ctgtggatcg aaaaggtcat ctcggtcggc aagatgagtt tcgatgaact
     4561 gagcctggct tcaggacttg cggttacgcg actcaaccgt atctttgccg ctctgcccac
     4621 gcacccggtt gcgatcgatc atgacaaacg acagtacctt tcggctgatg cgctggcggc
     4681 gctcgagcag aaccaatttt gcaacaactg tggtggtact ctcaaggttg aacacagcga
     4741 ccttctacag tgtccgtatt gccgcagcga ttacgccgtc gcgctcggaa aaaaatccaa
     4801 agcaaaaaac actccgccgc tggtcgtgag tacgctggca aattttctca aggcaagcgg
     4861 aatgatgctc atgattgtgt ttgtctttct cgcagccttc ggtgccatca cagaacttta
     4921 tgcgcgtttg catcgcaagg gtggcaccgg cggcgactgg gcagtggtca tcaccattgc
     4981 cattctgggc ggcgcggcgt atggtggctt tgcggcgctc gcgacccggc tgctttctgg
     5041 taagggttac gccctggttt cggcggtgct tgcaactctc gcccctctcg tctggcccat
     5101 ctttgcgctg cgcgcgctga tgtcgccgcg cagcaaattc tttttcgggc agttggattt
     5161 agcccgcata cgcgagtggc tttcagaaaa aaagtacatc ccccttattg aatttgcccg
     5221 ccggctgcgc gcgagcgaac tcgatgcaat gaacctcgcg cattacctca cgtcctcggg
     5281 tgagctcgac gcggtttatg accggcgcaa caacacgctg acagaacgct cgctctttcg
     5341 tgccaaggcc ggcgccaatt catgccaatc ctgcggggga gtggtctcgg tgatgaacgg
     5401 tgcccgcgtt tgccggtatt gtggaaccaa ggccacctaa gggtaaatgc cttcgcct
//
LOCUS       DAOQJF010000226         5449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8672_length_5449_cov_4.989803, whole genome shotgun sequence.
ACCESSION   DAOQJF010000226 DAOQJF010000000
VERSION     DAOQJF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8672_length_5449_cov_4.989803"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLF85_11725"
     CDS             <1..312
                     /locus_tag="PLF85_11725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNE20382.1"
                     /translation="EPQVSEPGYMRPVVIVQDDAFNRSRINTVIAVVITSNLKLAAAP
                     GNVPLPTTESGLAKDSVVNVSQIITLDKSCLTERAGSVARKTLLGIERGLLLVLGISM
                     D"
     gene            385..1896
                     /gene="thiC"
                     /locus_tag="PLF85_11730"
     CDS             385..1896
                     /gene="thiC"
                     /locus_tag="PLF85_11730"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNE20383.1"
                     /translation="MSDPTMQVPCRHVKLTNGDIIQLYETAGPQGYDAIQGIPKRREK
                     WIAARRAAGDKTPTQMYYAKKGVITEEMQFVAMREKMDAEFVRSEVARGRAIIPANIN
                     HTELEPMIIGRNFLVKINANIGNSALASSIEEEVEKLRWATKWGADTVMDLSTGKNIH
                     ETREHIIRNAPVPIGTVPIYQALEKVKGKAEELNIDIFLETLIEQAEQGVDYFTIHAG
                     VLLKYIPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFNEICEVMKKYDVSFSLG
                     DGLRPGSTADANDEAQFGELRTLGELTKIAWSHDVQTMIEGPGHVPMHMIKENMDLQL
                     DICHEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKED
                     VKTGVITYKIAAHAADLAKNHPGARERDDALSKARFEFRWEDQFDLSLDPETARKYHD
                     QTLPQDGMKKAHFCSMCGPHFCSMKITQDVRALEDGMAAKSEEFKAKGGEIYV"
     gene            1951..2463
                     /locus_tag="PLF85_11735"
     CDS             1951..2463
                     /locus_tag="PLF85_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20384.1"
                     /translation="MQVRRALESFVLQKKLNPRKLRNLLAVAGAAERLSSRPYMEQNE
                     LEGIVARFGVGPDVTTWGDFFAAEIATDHWEKTDAQFERICETVLFDFIAAAMVFSNK
                     GSEFAVSVLRDYQESLSKEAGLRTADDEEKIHLGILAGYYSQMGLEKSRLSDEDMEFF
                     ESFSLKTKTA"
     gene            complement(2503..3273)
                     /locus_tag="PLF85_11740"
     CDS             complement(2503..3273)
                     /locus_tag="PLF85_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20385.1"
                     /translation="MEIPYEKPFGEFLKFIRTAYPKAEYPEAFIPYIAHKATAGYALE
                     KYFPGLVSEFVARKGEFVYAISEPGWQNSPLNMKSTLTETEGGGYSLYANKSFILDAE
                     LGVFAVKYRDHFALVIVDTEKYREHRTALEMPEATYRVGPDKSTRHYRAEFTIALDIV
                     PFAELNKREILLFSRAVVFREQVAYAVLAAAEAAKEHDMSGIQSRVDLLCQRAKEEPG
                     QDDIAIAREILSASIVRILAKPGAHGFWKWLSRFVKPA"
     gene            complement(3294..4187)
                     /locus_tag="PLF85_11745"
     CDS             complement(3294..4187)
                     /locus_tag="PLF85_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNE20386.1"
                     /translation="MKKFSLFVLAYTLFVILFGAYVRISGSGAGCGEHWPSCEGEVIP
                     GDLIKNHAKAIEYTHRTTSGLSGIFVIILLIGVWRSKESSKSARRMALLSFLLIVSEG
                     LLGASLVLFRWVGLDASFGRGLMMPLHLGNTYLLLTALALTVKALSNPREELHLNRNR
                     AVQAGAALLLLTGMFGALAALGDTLFPGTRGFVTEGHTYLKLRNIHPLLAVFTIGYVL
                     TIAVRQRDDYPALISKYHLCDVVAFLTALQGLIGITNIVLSAPPYMQIIHLLGSDALW
                     IVYSYWLFSTARKNGKVLAGA"
     gene            4291..>5449
                     /locus_tag="PLF85_11750"
     CDS             4291..>5449
                     /locus_tag="PLF85_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE20387.1"
                     /translation="MTPARDLYTASTLQQLWQTQFSDLPFEAVVKQDVLRQGLSFTAA
                     ALEEADFKRKDYFIFTFDRAPLADMPDHKRVPEEIRIYTDSEVGGVSALQPTVISVRL
                     NPDSPYKVDRDAAGRIVLLLNNEVLSRVLYPPVPEYYKQQFSFDKPIGEVAPAIEWGY
                     LIYLTVFRNCQYFGKDEECQFCDINHNWRQQKREGRPYTGVKDPAHIVEALEKIAATD
                     STARAFTITGGSVTGKLQGKDEVEFYLQYPRAMSDKLKERFISKMVIQAWELDDCHKA
                     VEAGVKIYHPNYEVWDAELFSKICPGKERFIGRENWINRVVASAAAFGPENVIPNFVG
                     GVEMADGVGFTDEDKAVASTCEGLDFFMSKGIVPRFTTWCPEPFTPLGESAP"
BASE COUNT         1352 a         1449 c         1511 g         1137 t
ORIGIN      
        1 gagccacagg tctctgaacc aggttacatg cggccagtcg taattgtcca ggatgatgcg
       61 ttcaaccgca gccgcatcaa cacggtcatc gctgtggtga tcacctcgaa tctgaaacta
      121 gctgccgctc cgggtaatgt acctctgcca acaacggaaa gtggacttgc taaagattcc
      181 gtggtgaatg tatcgcagat tatcacactc gataagagtt gcctgacaga aagggcaggt
      241 tcggtggctc gcaaaacctt actcggcatt gagcgtgggc tgttgctggt gctcggaata
      301 agtatggatt agcagagctt catttccggt aggctatcag ggctaattcc tcgacggcct
      361 gtggcccgcc tccattcgtc caccatgagc gaccctacca tgcaggtgcc ctgccggcac
      421 gttaaactca ccaatggaga cataatacag ctgtacgaaa ccgccggccc gcagggctac
      481 gacgctatcc aaggcattcc taagcggcgc gagaaatgga tagcggcgcg tagggcagca
      541 ggcgataaaa ctcccacgca gatgtactac gccaagaagg gcgtgattac tgaagaaatg
      601 cagttcgttg cgatgcgcga gaagatggat gccgagtttg ttcgcagtga agtcgcgcgc
      661 ggtcgtgcga ttattcctgc aaatatcaac cacaccgaac tcgagccgat gatcattggc
      721 cgcaattttc tcgtgaagat taacgccaat atcggtaact cggcgcttgc ttcgtctatc
      781 gaagaagaag tcgagaaact gcgctgggcg acaaaatggg gcgccgacac ggtgatggac
      841 ctttcgacgg gcaagaacat ccacgaaacg cgcgagcata tcatacgcaa tgcacccgta
      901 cccatcggta ccgtgccgat ctaccaggcg cttgaaaaag tcaaaggtaa agcagaagaa
      961 ctcaatattg atatttttct cgaaacgctc atcgagcagg cagagcaggg cgttgactat
     1021 ttcacgattc atgcgggagt actgctgaag tacataccgc tgaccgcaaa gcgcgttacc
     1081 ggcattgtct cgcgcggtgg ttcgatcatg gccaagtggt gcctcgcgca ccataaagag
     1141 aactttctgt acacacactt caatgaaatc tgcgaggtga tgaagaagta cgacgtatcg
     1201 ttctcgctcg gcgatggctt gcggcccggt tcgactgcag acgctaacga cgaagctcaa
     1261 ttcggcgagc tgcgcacact gggcgaactg acaaagatcg cctggtcgca cgatgtgcag
     1321 acgatgatcg aaggtccagg gcatgtgccg atgcacatga ttaaagaaaa catggacctg
     1381 cagcttgata tctgccacga agcgccgttc tataccctgg ggcctctggt gaccgatatt
     1441 gcccctggct acgaccatat cacctcggcg atcggcgccg cgatgattgg ctggtatggc
     1501 actgcaatgc tttgttacgt gacgcctaag gagcacctgg ggctgcccga taaagaagat
     1561 gtcaagaccg gtgtcattac ctataaaata gccgcgcatg cggctgattt agccaagaac
     1621 cacccgggag cgcgcgagcg cgatgacgcg ctttccaaag cgcgctttga attccgctgg
     1681 gaagaccagt ttgatctctc actcgacccc gaaactgcgc gcaagtatca cgaccagacg
     1741 ctgccgcagg atggcatgaa aaaggcacac ttctgctcca tgtgcggccc gcacttctgt
     1801 tcgatgaaga tcacgcagga cgtgcgcgcg ctcgaagacg gcatggcagc gaaatcggaa
     1861 gaattcaagg caaagggcgg agagatctac gtataaacgg aaaaggcgga attcgcttat
     1921 gcgaatgccg ccttttccga caataacagt atgcaggttc gccgtgccct tgagagtttt
     1981 gttctccaaa aaaagctgaa cccgcgaaaa ttacgcaacc tgcttgcagt ggcaggtgct
     2041 gccgagcggc tttcatcgcg tccctacatg gagcaaaacg aactcgaggg cattgttgcc
     2101 cggttcggtg ttggccccga tgtcaccacc tggggtgatt tttttgccgc agaaatagca
     2161 accgaccact gggagaaaac agatgcgcaa ttcgaacgta tctgtgaaac tgtgctgttc
     2221 gattttatcg cagcagcgat ggttttttca aataaaggca gcgaatttgc cgtgagcgtg
     2281 cttcgtgact accaggagag cctgtcaaaa gaagctggtt tgcgcaccgc tgacgatgaa
     2341 gaaaaaattc acctcggcat actggcaggc tattacagcc agatgggact ggaaaaatcg
     2401 cgccttagcg atgaagacat ggaatttttc gagtccttca gcctgaaaac caaaacggct
     2461 taaccaatta gctcgcgccg gctggggggg gtacccagcc ccttacgcgg gcttcacaaa
     2521 ccgggataac catttccaga aaccgtgggc accgggtttt gccaggattc tgacaatgga
     2581 ggcagaaaga atttcgcggg ctatcgcgat gtcatcctgg ccgggttctt cttttgcccg
     2641 ctggcacaac aggtcgacac gagactgaat tcctgacata tcgtgctctt tcgcagcttc
     2701 tgcggcagcc agcacagcat aggctacctg ctcgcgaaaa acgactgcgc gcgaaaacaa
     2761 cagtatctcg cgcttgttga gctctgcaaa aggcacaatg tcgagcgcaa tggtgaactc
     2821 ggcgcggtag tgcctggtgc tcttgtcagg gcctacccgg taagttgctt cgggcatctc
     2881 aagagcggta cggtgttccc ggtatttttc cgtatcaacg atcacgagtg caaaatgatc
     2941 gcggtatttc accgcaaaca cgccgagctc cgcatcgaga ataaacgact tgttcgcgta
     3001 aagactgtag ccgccgcctt cagtctctgt cagagtactc ttcatgttga gtggtgaatt
     3061 ctgccagcct ggttctgaga tggcgtagac gaactcgcct ttgcgggcga caaattcaga
     3121 aacgagcccc ggaaaatatt tttcgagtgc gtagcccgca gtggccttgt gcgcaatata
     3181 gggtataaaa gcttccgggt actctgcctt aggataggcc gtgcgaataa atttcagaaa
     3241 ttcgccgaac ggcttctcgt aagggatttc catgttaaaa acttaaccgt gactcatgca
     3301 ccggcaagaa ctttcccgtt cttccgggcg gtggaaaaaa gccagtaaga atagacaatc
     3361 cagagtgcat cggaccccag caggtggatt atctgcatat aaggaggagc actcagtaca
     3421 atattggtga taccgatcaa accctgcagc gccgtcagga atgctaccac atcgcacagg
     3481 tggtacttac ttatgagggc tgggtaatca tcgcgctgcc gcaccgcgat agtgagcaca
     3541 tagcctatgg taaagactgc gagcagcggg tggatgttcc tgagtttaag gtacgtatgc
     3601 ccctcggtca caaaaccgcg agtcccggga aacagtgtgt cgccgagtgc ggcgagtgcg
     3661 ccaaacatgc cggtaagaag cagcaacgcc gcgcctgcct gtacagcgcg gttgcggttg
     3721 agatgtagct cctcccttgg atttgacagc gccttgaccg tcagcgccag cgcggtcagc
     3781 agcaggtacg tgttgccgag gtgcagcggc atcatgagcc cccgcccaaa cgaggcgtcg
     3841 agacctaccc agcgaaaaag gaccagggaa gcccccagca ggccctcgga gacgatgagc
     3901 aaaaaagaca gcagtgccat gcgccgcgcg ctctttgagc tttctttcga acgccagaca
     3961 ccgatcagca ggatgatgac gaaaatgccc gagaggccac tggtggtgcg gtgggtgtac
     4021 tcaattgcct tggcgtggtt tttgatcagg tcgccgggaa tgacttcgcc ttcgcaggaa
     4081 ggccagtgtt cgccgcaccc ggcacccgaa cccgaaatgc gcacgtaggc gccgaaaaga
     4141 ataacaaaca gggtataggc gagaacaaat agggagaatt ttttcataaa tagaatccga
     4201 aatatgtttg gccggcagag ccagccaaac atatttcggt ttaaggacga ccggacaagc
     4261 agaagctagg atttggctag tcgttaagac atgacccccg cacgcgacct ctacactgcc
     4321 agcaccctgc aacagctttg gcagacgcag ttttccgacc tgccgtttga agcagtggtg
     4381 aagcaggatg tgctgcgcca gggactttcg ttcaccgcag cagcgctcga agaggcagat
     4441 ttcaaacgca aagattattt catcttcacg ttcgaccgtg caccgctcgc cgacatgccc
     4501 gatcacaaac gggtgcccga agaaattcgc atctacaccg attcagaagt ggggggagtt
     4561 tctgcgctgc agccaactgt tatctcggtg cggctcaacc ccgattcgcc ttacaaggtt
     4621 gaccgcgatg cagccggtcg catcgtgttg ctcctcaata acgaagtgct ctcgcgtgtg
     4681 ctctatccac cggtgcccga atactataaa cagcaattct cttttgacaa accgattggc
     4741 gaggttgccc cggctatcga gtggggttac ctcatttacc tcacggtctt tcgtaattgc
     4801 cagtattttg gcaaagatga agagtgccag ttctgtgaca tcaaccacaa ttggcggcag
     4861 caaaaacgcg aaggacggcc gtacaccggg gtgaaagatc ccgcgcacat cgtcgaagcg
     4921 ctcgaaaaaa tcgcggcaac cgattcgacc gcgagggcat tcaccataac cggcggttca
     4981 gtcacgggca agttgcaggg taaagacgaa gtagaattct acctgcagta cccgcgcgcg
     5041 atgagcgaca agctcaaaga gcgttttatt tcaaagatgg tgatacaggc gtgggagctc
     5101 gacgattgcc acaaagcggt ggaagcgggg gtgaaaatct accatcccaa ctacgaggtg
     5161 tgggatgccg agctcttcag caaaatttgc cctggcaaag aacgtttcat cggccgcgag
     5221 aactggatta accgtgtcgt cgcatctgcc gcagcatttg gccctgagaa cgtgatcccg
     5281 aatttcgtcg gcggcgtcga gatggccgac ggcgtgggct tcaccgacga agacaaggct
     5341 gtcgcttcga cctgcgaggg gttggatttc ttcatgagca aagggattgt tccgcggttc
     5401 accacctggt gccctgaacc cttcacgccg ttgggcgaaa gcgcgccac
//
LOCUS       DAOQJF010000227         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8674_length_5448_cov_5.477842, whole genome shotgun sequence.
ACCESSION   DAOQJF010000227 DAOQJF010000000
VERSION     DAOQJF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8674_length_5448_cov_5.477842"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="PLF85_11755"
     CDS             complement(<1..1889)
                     /locus_tag="PLF85_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE20388.1"
                     /translation="MTRFALIGLLSLFFSFCGGGFGDPGMGDGRERFSVFFSEPGRDQ
                     DTMIDKKVDDELVRLIDGAERYMYLAVYNFNKTSVVQAVLRAFQRNIDVRVVGDIDEF
                     YTSGYQTMYFNNINMTLGNASGIQHNKFAVVDDRYVFMGTGNISDTDMVRNNNNWYII
                     ENAQLVKLYKDEFLQMHNGLFGAQKRQRSTSTQIVLNNNPFELYFSPYQGDEAMDRII
                     ALVESAQTTIHYMIFAHTHDELDAAMVRMARQRGIPVYGIHDSTFVSGISEEAPKMYS
                     AGFNNDGSMSPTGPFVRWDGNENTSIKNNPAHGGKMHCKTVLIDAGTPNARMATGSFN
                     WSNNAINNNDENVIIVNQARVVNTIYEQWKGAWGIANDMALRVQRRGHTANAGDVLIS
                     EVGWAGATDGSNIENGDDFIEIFNNSAAPIDLSHWAIQWGTNDKRNLYPVPDPHNWYY
                     ENKNACSGFSEYLTAKPNIICPGQTRIFYNNKPSAFAETGADEIITYNEDGAEIRNTT
                     GGSLTTEHFKFSGTKNFKISGSSFRIRLYDTAMNLIDEAGDGGTAPAGIYSDYTAPKQ
                     TFSMERRGYNSVTQRFTAQQSGRLSSAWFTHRSAEPYTCANRQDYSGTAFDGCTTKAT
                     NTFSSA"
     gene            complement(1920..4241)
                     /locus_tag="PLF85_11760"
     CDS             complement(1920..4241)
                     /locus_tag="PLF85_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNE20389.1"
                     /translation="MPENRTKRTVALLGAGYLQQPFIREVKELGLRVIALDQNAHAYA
                     MHDADEFMQVQIADAEAVIAVLAPLRDQLGFVTTVATDFSHIVGRVNDELGLPGLNTQ
                     QGIVLTHKGKMRDFCAAHGHAHPAYFYGKSKDDIASWVKSNPNTAGFVIKPVQNMGAR
                     GVMFIRGVDDLSYAHEFAASQDKLGEAIIEHYVPAREISVDALCTQGQVFLTGVADRL
                     IEIKDTHFFIENGHTLPGNLTTAEYTAILHTLQRYADSLAKLANRPYHGALKGDLRLT
                     DDGRVVIGEIAGRLSGGFMSTHTYPAAHGRSLMQLFAQLLLGEVETPLTLEASVATNG
                     ISIERSLYAEPGVLSGLPDGQQLEALRAEFGPQGLIELRCNYQNGDLVENLQNNIGKV
                     FHTVIHAATLAKAEEVFARLRQHLTYQTKTPPYDQRAMAKVARARFNNDFCWVCKVCD
                     GGYCASGVPGMGGRGEMNSFRDNITSLGEYRITPTYTEKGPVGPASTAFDFLRSPKLR
                     FAGPVLSAPITGSVTNMGGSITEYDYALETGNAMKQLQLLPTFGDGASADKYRVGLHA
                     IEQLGYGIPVFKPRTEQRELIRRIESAARSGATAWGIDIDGVSFKTMVLKSAATSHKS
                     LAELRELKIASTLPMVVKGVMTQADAAVAIEAGASAIVVSNHGGRVADGMPGVARVLP
                     QIARFVREQAPHVQVLADGGVRSGADIFRMLALGAEAVLVGRPVAIMAVGYGRTGVAS
                     MLRHWLDELRSIMQVHGVARLSDISAQHITKFI"
     gene            complement(4241..4402)
                     /locus_tag="PLF85_11765"
     CDS             complement(4241..4402)
                     /locus_tag="PLF85_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20390.1"
                     /translation="MNRLATLEQLLLIRDATFNGPPLVKIYREILKAIKPETDAGLHS
                     FVIEKTETR"
     gene            complement(4399..5130)
                     /locus_tag="PLF85_11770"
     CDS             complement(4399..5130)
                     /locus_tag="PLF85_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20391.1"
                     /translation="MILYTAQLPSRNCGINLAFEEAFFHVAKPVRSLYLFFYENNDAL
                     VLGKSLVTEEEVYEHKGPPVYRRISGGGSVLHCRGNLNYSLFLSLEDFPELMNVSLSY
                     EKILGGIAHVLGHSVAQRGYSDLTIRCRGADRKFSGNAQCRKRGWVMHHGTLLYTKAA
                     LKRIPLYLRPPPKQPAYREGRRHRDFMTNVLPCYERTQLIWRVRQGIARSFGAELRVM
                     PEHEIANLAHNPPFQAERPGRKGFP"
     gene            complement(5127..>5448)
                     /locus_tag="PLF85_11775"
     CDS             complement(5127..>5448)
                     /locus_tag="PLF85_11775"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNE20392.1"
                     /translation="AALMQSLEPQRFVQLTDALWWSIVTLTTVGYGDLVPESLPGKLV
                     AGVLMVFGIGIIALPTGVLGASMTKLMLEDRSRKVKECTRCGEQEHLTVARFCHKCGE
                     RLPE"
BASE COUNT         1141 a         1472 c         1553 g         1282 t
ORIGIN      
        1 gccgagctga aggtattcgt ggccttggtg gtgcagccgt cgaatgcggt gccgctgtaa
       61 tcctgcctgt tcgcgcaggt gtagggttca gcgctacggt gcgtgaacca ggcggagctc
      121 aggcgtcccg actgttgtgc cgtaaaacgc tgtgtcaccg aattgtaccc ccggcgctcc
      181 atcgaaaatg tctgcttcgg cgctgtgtag tcgctgtaaa tacccgccgg agcggtgccg
      241 ccgtcgccag cctcgtcgat caggttcatc gccgtgtcgt agaggcgaat acggaacgaa
      301 gatccggaga ttttaaaatt tttagtgccg gaaaatttga aatgttctgt cgtcagcgag
      361 ccgccagtgg tattgcgtat ctctgcgcca tcttcattgt aggtaataat ttcatcggca
      421 ccggtttcgg caaaagccga cggtttattg ttgtagaaaa tgcgcgtctg cccgggacag
      481 atgatattcg gttttgccgt caggtattcg ctgaaacccg agcaggcatt cttgttctcg
      541 taataccagt tatgcgggtc gggtaccggg tagaggttgc gcttgtcgtt ggtgccccac
      601 tgtattgccc agtgcgaaag gtcgattggc gcggcggagt tattgaagat ttcgatgaaa
      661 tcgtcgccgt tttcaatatt gctcccgtcg gttgcgccag cccagccaac ctctgaaata
      721 agcacatcac ccgcgttcgc ggtatgcccc cgccgctgca cgcgcagcgc catgtcgttg
      781 gcaatacccc aggcgccttt ccattgttca tagatggtat tgaccacccg ggcctggttc
      841 acgataatta cattttcgtc attattgttg atcgcgttgt tcgaccaatt gaatgacccc
      901 gttgccattc ttgcattggg cgtaccggca tcgatcagca ccgtcttgca gtgcatcttg
      961 ccgccgtgcg cagggttgtt cttgatcgag gtgttttcgt tgccgtccca gcgtacgaag
     1021 gggcccgttg ggctcatcga gccgtcgttg ttgaaacccg ccgaatacat cttgggcgct
     1081 tcttcagaga tacccgagac gaaggtcgaa tcatggatgc cataaacggg aataccccgc
     1141 tgccgtgcca tacgaaccat cgcggcgtcg agttcatcgt gagtgtgcgc gaatatcatg
     1201 taatgaatag tggtctgggc agattctacc agcgcaataa tacggtccat ggcttcgtcg
     1261 ccctggtagg gagaaaaata cagttcaaac ggattgttgt ttagcacaat ctgcgtactc
     1321 gtactgcgct gccgtttttg cgcaccaaag agcccattgt gcatctgcaa gaattcgtct
     1381 ttgtacagtt tcaccagctg cgcattttcg ataatatacc agttgttatt gttgcgcacc
     1441 atgtcggtat cggatatatt tcccgtgccc ataaagacat agcggtcatc gacgacggcg
     1501 aatttattgt gctgaatacc tgaagcgttg cccagcgtca tattgatgtt gttgaaatac
     1561 atcgtctggt agccgctcgt gtaaaattcg tcgatatcgc ccaccacgcg gacatcgatg
     1621 ttccgctgga aggcacgcag taccgcctgc acgacagaag ttttgttgaa gttgtagacc
     1681 gcgaggtaca tgtagcgttc agccccgtcg atcagccgta ccagctcatc gtcgaccttc
     1741 ttgtcgatca tcgtgtcctg gtcgcggccg ggttcagaaa agaatacgga aaagcgttcg
     1801 cgcccgtcgc ccatgccggg gtcgccaaat cctccgccgc agaaagaaaa gaacagggat
     1861 aagagaccga tgagggcaaa cctcgtcacg ggtagacgat tgccagacaa agagcggcgt
     1921 caaatgaatt tcgtgatatg ctgtgcgctg atgtccgaca ggcgtgcgac accgtgaacc
     1981 tgcataatcg agcgcagttc atcgagccag tgccgaagca tgctggcaac gccggtacgg
     2041 ccgtaaccga cagccatgat ggcaacggga cgaccgacca aaactgcttc ggcgccgagt
     2101 gccagcatac ggaaaatatc tgcgcccgat ctcactccgc catcggcgag tacctgcaca
     2161 tgcggcgcct gttcacgaac gaacctcgca atctgcggca agacccgggc gacacccggc
     2221 ataccatctg cgacgcggcc accgtggttg ctgaccacga tggcgctcgc accggcttcg
     2281 atggcgacgg cggcgtcggc ttgtgtcata actcccttga ccaccatggg cagggtagag
     2341 gcaattttta attcacgcag ttctgcaagg ctcttgtgcg aggtggcggc gctcttcagt
     2401 accatcgtct tgaaagaaac accgtcgatg tcgatgcccc atgcggttgc acccgagcgc
     2461 gcggccgatt cgatacgccg gatgagctcc cgctgctcgg ttctgggttt gaataccgga
     2521 atgccataac caagctgttc tattgcgtgc aggccaaccc ggtatttgtc agcagacgca
     2581 ccatcgccga atgttggcag cagctgcaat tgcttcatcg cgttgcctgt ctctagtgcg
     2641 taatcgtatt cggtgataga accacccata ttcgttacgc tgcctgtgat gggggccgag
     2701 agcacagggc ccgcgaagcg gagtttcgga ctgcgcagaa aatcaaaggc agtggatgca
     2761 ggtccgacgg gtcccttttc tgtataagtg ggagtaatgc ggtattcacc gagcgaggtt
     2821 atattgtcgc gaaacgagtt catttcaccc cggccgccca tgccgggcac acccgaggcg
     2881 cagtagccgc cgtcgcatac cttacagacc cagcagaagt cgttattgaa ccgggcgcgc
     2941 gcaacttttg ccattgccct ttggtcgtag gggggagttt tcgtttggta tgtgaggtgc
     3001 tgccgcagcc gggcaaatac ctcctccgcc ttggcgagcg tggcggcatg gataaccgtg
     3061 tgaaagacct tgccgatgtt gttctgcagg ttttctacca ggtcgccgtt ctggtagttg
     3121 cagcgcagct ctatcaaacc ttgcgggcca aattctgccc gcagggcttc aagctgttgt
     3181 ccatccggca ggccgctgag aactccgggt tcggcgtaaa gcgaccgctc gatggaaatt
     3241 ccgttcgttg ctacagaagc ttcgagggtg agaggggtct ccacttcacc gagcaatagt
     3301 tgggcaaaaa gctgcatgag gcttctgcca tgcgctgccg ggtaggtatg cgtgctcatg
     3361 aaaccacccg acaggcggcc ggcgatctca ccgataacta cccgaccgtc gtcggtgagt
     3421 cgcaggtcgc ctttgagcgc gccgtggtaa ggcctgttgg caagttttgc cagtgaatcg
     3481 gcatagcgct gcagcgtgtg aagaattgcc gtgtattcgg cagtggtcaa atttcccggc
     3541 agcgtgtggc cattttcaat gaaaaaatgc gtatctttta tttcaatcag ccggtcggcc
     3601 acgccggtga ggaatacctg gccttgcgtg caaagtgcat cgactgaaat ctcccgtgcc
     3661 ggaacatagt gttcaataat tgcctcgccg agtttatcct ggcttgcagc gaactcatgg
     3721 gcgtaagata ggtcgtcgac gccccggata aacatgacgc cgcgcgcgcc catgttctgc
     3781 acgggtttga tgacgaagcc tgccgtgttg ggatttgact ttacccacga agctatgtcg
     3841 tccttactct tgccgtaaaa gtaagcaggg tgggcatgac cgtgagcagc gcagaaatcg
     3901 cgcatttttc ccttgtgcgt cagaacgatg ccctgctgag tattgagacc cggcagcccc
     3961 agctcgtcat tcacccgacc cactatgtgc gagaaatcgg tggcgacggt cgtgacaaag
     4021 cccagttgat cccgcagggg cgcgagcacc gcaattacag cttcagcatc ggcaatctgt
     4081 acctgcatga attcatcggc gtcgtgcatg gcgtatgcat gcgcattctg gtcgagtgcg
     4141 atgaccctga gcccgagctc ctttacttcg cggatgaacg gctgctgcag ataacctgcg
     4201 cccagtagcg cgacggtccg cttggttctg ttttctggca tcagcgtgtt tcagtttttt
     4261 cgatcacaaa ggagtgcaat cctgcatcgg tttctggctt tatagcttta agtatctctc
     4321 ggtaaatctt taccaggggc gggccgttga aggtggcatc gcggatgagc agcagctgtt
     4381 ctaacgtggc caatcggttc atgggaaacc ttttctgccc gggcgttctg cctgaaaagg
     4441 tggattatga gccaggtttg cgatctcgtg ttcaggcatg accctcagtt ccgcaccaaa
     4501 cgatcgtgca ataccctggc gaacccgcca gatgagttgc gttctttcat agcagggcag
     4561 tacgttcgtc atgaagtcgc ggtgccggcg tccctcgcgg taggcaggtt gtttcggggg
     4621 cgggcgaagg tatagtggaa tccgtttcag ggcggccttc gtgtagagca gcgtcccatg
     4681 gtgcataacc cagccacgct tgcggcactg cgcattgcca gagaatttgc ggtcggcacc
     4741 gcggcagcga attgtcaggt cggagtagcc tctttgtgcg acagaatggc ctagtacatg
     4801 ggcaatgccg ccgagaattt tttcataact cagggaaacg ttcatcagtt cgggaaagtc
     4861 ctcgagtgat agaaacaggg aatagttgag gttgccccgg cagtgtagaa ccgagccgcc
     4921 gcctgaaata cgccggtaaa ccggggggcc cttatgttca tagacttcct cttcggtgac
     4981 cagcgacttg ccgagcacta gcgcatcgtt gttctcatag aaaaagagat acagggaccg
     5041 gacaggcttc gcaacgtgga agaatgcttc ttcaaaggca aggtttattc cgcagtttcg
     5101 cgagggcaac tgtgccgtgt agagaatcat tcgggcagac gttcgccgca tttgtggcag
     5161 aagcgggcga cggtaaggtg ttcctgttcg ccgcaccgcg tgcactcttt cacttttctg
     5221 cttctatctt caagcatcag cttggtcatt gatgcaccca gcacgcccgt gggcagcgcg
     5281 atgatgccga taccaaagac catgagcacg cccgcgacca gcttaccagg caggctttca
     5341 ggtactaaat caccgtaacc tacggttgtc agtgtgacaa tcgaccacca gagtgcatca
     5401 gtaagctgca caaaacgttg tggttcgagt gactgcatca atgcggct
//
LOCUS       DAOQJF010000228         5426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8719_length_5426_cov_4.297524, whole genome shotgun sequence.
ACCESSION   DAOQJF010000228 DAOQJF010000000
VERSION     DAOQJF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8719_length_5426_cov_4.297524"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLF85_11780"
     CDS             <1..294
                     /locus_tag="PLF85_11780"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNE20393.1"
                     /translation="LGGCLFFLDIWLTWIYFALLESSYYQGTPGKLALGIRVWHKDKR
                     ALTFEEATVRYFAKILSRLTLLIGYILCAFSSRKQALHDFVASSVLVVQRGEI"
     gene            complement(313..834)
                     /locus_tag="PLF85_11785"
     CDS             complement(313..834)
                     /locus_tag="PLF85_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE20394.1"
                     /translation="MAEKQKHIKTLFLLRHAKAEAHDLKKNDHERRLIDKGVRHAEKM
                     AKLVDSFRFKPEAIVTSSAARAEETAAIFADTLGLKQQLTVNDVLYSASQQIYLQTIQ
                     QLPDDLDNVMIVGHNPVLEDLLVSLSSRSPFHCKMPTCGLAVVHFHVTLWSEIRPATG
                     IMRVLLYPKLFAE"
     gene            complement(836..1876)
                     /gene="argC"
                     /locus_tag="PLF85_11790"
     CDS             complement(836..1876)
                     /gene="argC"
                     /locus_tag="PLF85_11790"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNE20395.1"
                     /translation="MSTPVRVGILGAGGFTGQELIRILGRHPHVELVHITSSEYVSKP
                     LAEVFPGLASTATKDLVFAPHPQQTKDVGKIDAIFLATPDDVALRWGPQFVAAGIKVI
                     DISGAFRLLDKAEFKEYYGLDHDQPAALSQAVYGLTEVNREKIRSAQLVANPGCYPTA
                     ALLPLYAYRDLIDTSRPLIIDAKSGSSGAGGRKEKDGLGYSTVYENFRAYKTAKHQHA
                     PEIAQEFSRMTGSPATLRFTPHLLPMYRGILSNTYAYLKPGVNEKACADAALTLSKNE
                     LFVRYRANPDQVELRSVQHSNFCDFAHYIDEKSGMIQIVSAIDNLMKGAAGQAVQNMN
                     LMFSLPETAGVY"
     gene            complement(1873..2748)
                     /locus_tag="PLF85_11795"
     CDS             complement(1873..2748)
                     /locus_tag="PLF85_11795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE20396.1"
                     /translation="MYYLDRAFRVFAVVLGLTVSTACSKTPKNKAEAMKEVKEKMRKD
                     TNPIAVIETSKGNITIELYRHAAPNTVGNFISLARGEKEFTDADGKPAKRPYYDGLIF
                     HRVIPNFMIQGGDIKGNGTGGPGYQFADEINAKALGLDKMKVATSPMAMQEVQQVARN
                     EVFKKLNIKSQADLNAKMKEAEAMFAAEQAKWKDKSLEELYTARGVTYTPNLESESNT
                     KGSLSMANAGPNTNGSQFFISVADNTYLDGKHTVFGRVIAGLDVAEQITQVDRDQQDR
                     PKTPVKMTKVTILEN"
     gene            complement(2748..3497)
                     /locus_tag="PLF85_11800"
     CDS             complement(2748..3497)
                     /locus_tag="PLF85_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNE20397.1"
                     /translation="MKIKLKIWRQKDKEDKGGFKDYQLDNVNEHMSFLEMLDVLNEDL
                     SHKGEEPVAFSHDCREGICGSCGTVINGRPHGPEHGTATCQLHMRKFSDGDSITVEPW
                     RARAFPVLKDLVVDRSAFDRIQQAGGYITVNTGSAPEANLTPIPKPVADEAFLAAACI
                     GCGACVAACKNASAMLFVAAKVSHLALVPQGRAERAERVRKMVAAMDAEGFGNCTNTG
                     ACEAECPKEISITHIARMNREYMSASLVSEY"
     gene            3552..5123
                     /locus_tag="PLF85_11805"
     CDS             3552..5123
                     /locus_tag="PLF85_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNE20398.1"
                     /translation="MGLLATAMQPVLNRDQMRRYDALVISEGKLPGIVLMENAGRGAF
                     ECLQRLLAGHVAGLPHVLIVCGPGNNGGDGYVVARHLLALRNMQATVEVLILADRSQI
                     QGDAAINLESLLALAPATVRFCAPTSPQLEEALARADFVVDAIFGTGLSRPVAGEMLQ
                     AIERLNHATAVRVALDVPSGLDCNTGQQLGGCVQAHHTVTFGYPKPGLLTPQGKDKSG
                     ELHSVGLGIPDSTILAQTGQAAALLSGDDILPVLSPRHASTFKHRSGDILVVAGSVGK
                     TGAAKLAAHAALRAGAGLATTCTWQEALPALASEVTEIMLSALSETRITEDLALALAK
                     RHAVLVGPGFGTGEAARHALQYILENASVPLVIDADALTMVAQHPELAERIPPNAVLT
                     PHSGELARLLQTTSQQIEADRYAAVASAAEKFRCTVILKGAHTLIGTAQQIVVSPWAN
                     PVLATAGSGDVLAGIMTALAAQIEIFAAAKAAVYLHGLAGQIWSNTKRADRGMLASDI
                     ADTLPEAIARLLGKS"
     gene            complement(5177..>5426)
                     /locus_tag="PLF85_11810"
     CDS             complement(5177..>5426)
                     /locus_tag="PLF85_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20399.1"
                     /translation="DVRELLAFFARQLTADENKKLAAQLQGMSVRAIRDVCLRAERQL
                     AREQIEQGTASTTPAAPTLAYYVRTLAEYHLGRPVNQA"
BASE COUNT         1096 a         1543 c         1535 g         1252 t
ORIGIN      
        1 cttggcggct gcctgttctt cctggatatc tggctcacct ggatatactt tgccctgctc
       61 gaaagctcct actaccaggg tacacctggt aaactggcgc ttggcatcag ggtctggcac
      121 aaggataagc gcgccctgac gtttgaagaa gcgaccgtgc gttactttgc gaaaattctc
      181 agcaggctga cactactcat cggctacatt ctctgcgcct tcagttcgcg caaacaagcc
      241 ctgcacgatt ttgtggccag ttcagttctt gtggttcagc gcggcgagat ctgaccgcac
      301 tcgacctttc tgctactcgg caaaaagttt cgggtaaagt aaaacgcgca tgatgccggt
      361 cgctggccgg atttctgacc agagtgtcac atgaaaatga accacggcaa ggccacaggt
      421 cggcatcttg cagtggaatg gcgagcgcga actgagcgaa accagcaggt cttcgagtac
      481 cgggttatgg cccacgatca tcacgttatc caggtcatcg ggtaattgct gtattgtttg
      541 caggtagatc tgctgcgatg cggaataaag cacatcattc actgtcagct gctgtttcag
      601 cccgagcgta tcggcaaaaa tggcggccgt ttcttcagcg cgcgcagcag aagaagtgac
      661 aattgcctct ggcttaaagc ggaacgaatc gaccagcttt gccatttttt ccgcgtgccg
      721 tacaccttta tcaatcaggc ggcgctcatg atcatttttt ttgagatcat gcgcttcggc
      781 cttcgcatgc cgtagaagga aaagggtttt gatgtgtttt tgtttttccg ccaactcagt
      841 atacccccgc cgtttcaggc aatgagaaca taaggttcat attttgcaca gcctgccccg
      901 ctgcgccttt catcaggtta tcaattgccg atacgatctg tatcatgccg cttttttcat
      961 caatgtaatg cgcgaaatca caaaaattgc tgtgttgcac tgagcgcagc tcgacctgat
     1021 cgggatttgc acggtagcgc acaaagagtt cattctttga aagtgttaat gccgcgtcgg
     1081 cgcaggcttt ttcgttcact cccggcttca ggtatgcata ggtatttgac agtatgcccc
     1141 ggtacatggg cagcaggtgg ggagtaaagc ggagggtagc gggcgagccc gtcatgcgcg
     1201 agaactcctg cgcaatttcg ggggcatgct ggtgtttcgc tgttttgtac gcgcgaaagt
     1261 tttcatagac ggtagaataa cccagtccgt ctttttcttt gcgcccgcct gcccccgacg
     1321 agccgctctt agcatcgata atcagcggac ggctcgtatc gatcaggtcg cggtacgcat
     1381 agagcggcaa aagcgccgca gtgggataac aaccggggtt tgctaccagc tgcgccgagc
     1441 ggattttttc ccggttcacc tcggtaaggc cgtaaaccgc ctggctgagt gctgccggct
     1501 ggtcatggtc aaggccatag tactctttga actccgcctt atcgagcagc ctgaaagcgc
     1561 cggaaatatc tatgactttg atgcccgcag cgacaaactg tggcccccac cgcaaggcca
     1621 cgtcatcggg tgtggcgaga aatatggcgt cgattttccc tacatccttc gtctgctgcg
     1681 gatggggagc gaagaccagg tcttttgttg cggttgaggc aagccccggg aaaacctctg
     1741 caagcggttt cgagacatac tcagacgaag tgatatggac cagttcgaca tgaggatgcc
     1801 tgccgagaat ccggatgagt tcctgcccgg taaagccacc ggccccgaga ataccgacgc
     1861 gaaccggcgt gctcaatttt cgagaatagt gactttagtc atcttgaccg gggtcttggg
     1921 acgatcctgc tggtcacgat ctacttgggt gatctgctcg gctacgtcaa ggcctgcaat
     1981 gacgcgcccg aacacggtgt gttttccatc gaggtacgtg ttgtccgcaa cgctgataaa
     2041 aaactggctg ccgttggtat tcggcccggc gttggccatc gaaagcgaac ctttggtatt
     2101 ggattctgat tcgaggtttg gcgtgtaagt gacgccgcgc gcggtgtaga gttcttcgag
     2161 actcttgtct ttccactttg cctgctcggc agcgaacatt gcttcagcct ctttcatttt
     2221 cgcgttgagg tctgcctgac tcttgatatt cagcttctta aaaacctcgt tacgcgcaac
     2281 ctgctgaact tcctgcatgg ccatcggtga ggtggcaact ttcattttat cgagcccgag
     2341 tgccttggcg ttgatctcgt cggcaaactg gtaccccggc ccgcctgtac cattaccctt
     2401 gatatcgcca ccctgaatca tgaagttcgg tatgacgcgg tggaaaatca ggccatcata
     2461 gtatgggcgc tttgcaggtt tgccatcggc atctgtgaat tctttttctc cgcgcgcgag
     2521 gctgatgaag ttgcccacgg tattgggcgc agcatggcgg tacagctcaa tggtgatatt
     2581 tcctttgctg gtttcgatga ctgcaatggg gttggtatcc ttacgcatct tttctttgac
     2641 ttctttcata gcttccgcct tgttttttgg agttttgctg caggccgtcg agacggttag
     2701 tcccaacact acagcaaaaa cccggaacgc gcgatcgagg tagtacatca atattcgcta
     2761 accagcgaag cgctcatata ttcgcggttc atgcgcgcaa tgtgcgtgat agatatttct
     2821 ttcgggcact ctgcttcgca cgcaccggta ttcgtacagt tgccgaaacc ttcggcgtcc
     2881 atggcggcga ccattttgcg cacgcgttct gcgcgttctg cgcggccctg gggcaccagc
     2941 gcaaggtgcg aaacctttgc cgcaacaaag agcattgctg aagcattctt acaggccgca
     3001 acgcatgctc cgcaaccgat acaggccgcc gcgagaaacg cttcgtcggc taccggcttg
     3061 ggaataggag tgaggttggc ttcaggggca ctacccgtgt taacggtgat gtagccgccg
     3121 gcctgctgta tacggtcaaa agccgagcgg tcaacgacaa ggtctttgag aacgggaaaa
     3181 gcacgcgcac gccatggttc tacggtgatg gaatctccgt cgctgaactt gcgcatgtgc
     3241 agctggcagg tagccgtacc gtgctcagga ccgtgcgggc ggccgttgat caccgtaccg
     3301 catgagccgc agataccttc gcggcagtcg tggctgaagg caaccggctc ttcgcctttg
     3361 tgcgagaggt cttcgttgag tacgtcgagc atttcgagaa acgacatgtg ctcgttgacg
     3421 ttatcaagct ggtagtcttt gaaaccacct ttgtcttctt tgtctttctg gcgccagatc
     3481 ttaagtttga ttttcatgcg gtttaccgtt agttgcaaaa ttctttttgg cattacggca
     3541 taaagcgaaa aatgggactg cttgccaccg ccatgcaacc cgtgctcaac cgcgaccaga
     3601 tgcgccgcta tgacgccctg gttatttcag agggtaagct gcccggcata gtcctcatgg
     3661 aaaatgcagg tcggggggct tttgaatgcc tgcaacggct gttggcgggc cacgtggccg
     3721 gccttcccca cgtcctgatc gtctgcggcc ccggcaacaa cgggggcgat ggttatgtgg
     3781 tggcgcgcca tctgctggcc ctgcgcaaca tgcaggccac agtcgaggtt cttatactcg
     3841 cagaccgcag ccagatccag ggcgatgccg ctattaacct cgaaagcctg cttgcgctcg
     3901 cgccggcaac cgtcaggttc tgcgctccca ccagcccgca gctcgaagaa gccctggcca
     3961 gggccgattt tgtcgtcgat gcgatattcg gtaccggcct atcgcgcccg gtcgccggcg
     4021 aaatgctgca ggccatcgag cgcctcaacc atgcgactgc agtgcgagtc gctctcgacg
     4081 tgccatcagg cctcgactgc aacacgggcc agcaactcgg cggctgcgta caggcacacc
     4141 acacggtaac ctttggttat cccaaacccg ggctgctgac gcctcagggc aaagacaagt
     4201 cgggggagct ccactcagtt ggacttggta ttcccgactc aacgattctc gcccaaactg
     4261 gtcaggcggc tgccctgctc tccggagacg acattctgcc ggtactttct ccgcgccatg
     4321 ccagtacctt taagcaccgc tcgggcgata tcctcgtggt tgcaggttct gtggggaaaa
     4381 ccggcgccgc caaactcgcg gcgcatgcag cgctgcgtgc gggtgcggga cttgcgacca
     4441 cctgtacctg gcaggaagca ctgcccgcac tggcaagcga ggtcaccgag attatgctca
     4501 gtgcgctatc cgaaacgcgc attacggagg accttgcgct tgcactggca aaacggcatg
     4561 ccgtactcgt cggccctggc tttggtaccg gagaagcggc gcgccatgca ctgcaatata
     4621 tcctggaaaa cgccagcgtg cccctcgtga tcgacgccga tgcgctgacc atggttgcgc
     4681 aacacccgga actcgctgag cgcattccgc caaacgcggt actgaccccg cacagcgggg
     4741 agctcgcacg cctgctgcaa acaacctcac agcagattga ggcagatcgt tacgcggcgg
     4801 tagcctctgc cgcggaaaaa tttcgctgca cggtcatcct caagggcgca cacacgctga
     4861 tcggcactgc gcagcagatc gttgtttcgc cgtgggcaaa ccccgtgctg gcgactgcgg
     4921 gttcgggcga cgtacttgct ggcataatga cggcgctcgc cgcccaaata gaaatatttg
     4981 cagcagccaa agcagcggtc tacctgcatg gccttgcggg ccaaatctgg agtaacacaa
     5041 aacgcgccga ccgtggcatg ctcgccagcg acatcgccga cactctgccc gaagcgattg
     5101 cgcggctgct cgggaaatcc taacaggctc ctgagaaagg agccagaagc cggtgcggga
     5161 gtttttccgg aacctgctaa gcctggttca cgggacggcc gaggtggtat tccgccaggg
     5221 tccgcacata gtaagcgagc gtgggcgctg ccggcgttgt cgaagcagtg ccctgctcta
     5281 tctgctcgcg ggccagctgc cgttcggcgc gcaggcagac atccctgatg gcgcgcaccg
     5341 acatgccctg cagctgtgca gcgagcttct tgttctcatc ggctgtcagc tggcgggcaa
     5401 agaatgccag cagttcgcgt acatcg
//
LOCUS       DAOQJF010000229         5410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8760_length_5410_cov_5.243697, whole genome shotgun sequence.
ACCESSION   DAOQJF010000229 DAOQJF010000000
VERSION     DAOQJF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8760_length_5410_cov_5.243697"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="PLF85_11815"
     CDS             complement(<1..1875)
                     /locus_tag="PLF85_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20400.1"
                     /translation="MTRRILAALSLSKGRILLVLAAGTLFAEPVGWGDPSLVDRRAEE
                     FGVCCHEGEARLYAVTTSPKGAFNKEAPTTVELALVYSEGGKFERRVLTEVTLAVADQ
                     LFPLHPSVSALGDEILVAWQETSPQGNASGIYFTYSASGPTGFTTKQVLPSTAGKLNA
                     ILPLAKMTGPGQHVILYQEPAAVNRFQLVAAYGGRGVFSGLSPVAEISGGTRGALFPA
                     TIRRGKRIDIFYQNRAEATLIDDIFRAFSQDGGVSWGGNVRVTSNGFQNFSAQVTDTK
                     DKLVFIWQSNPNKVWSIFAAPEGSEPIAVNDNASPSYLPAIVAGGDTVVAAWQDTRNG
                     APQIYAKFLDRPDNSFVGVDHRVSKEGIAAKPIEFARLGNRPFLFYGCGPGLCVREAD
                     TTADPVRIFSRTHAIGQVSKSSDAIFNWPKIDDISGVESFAWVIDDTRETNPDLFNMN
                     ARATQITVPGLNGGAYYLHIKYRDKAGNVSPVTHYPFVVDSVPPSRPIITSPSHDNGI
                     PDSRQEVVLRFNAADDSGIATYRYAFAEKLPRVFADTTSSTELTFNDVPPGKYLFAVE
                     AVDLGGNVSERAFYRIEIGVNERNDLTIRHNAEADVITRPDIAFTIQDNGGRGIREVY
                     Y"
     gene            complement(1872..2828)
                     /locus_tag="PLF85_11820"
     CDS             complement(1872..2828)
                     /locus_tag="PLF85_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE20401.1"
                     /translation="MFIRFWGVRGSLATAITARQVEDKIRRVLELASPADLLSKEAIE
                     NFISNLPLSIRGTYGGNTTCVEIQTSDNKTIVIDAGTGLRALGNALMARGKMTGKDDM
                     AFFFTHSHWDHIQGLMFFVPVFIPGNKLNFYSCFPDIEARLRYQMITTHFPLTFDELN
                     CEKSFHHFAETESVDVYGLTVSAKAVRHPGGCYSYKFRQPNGKTFIFCSDAEFNLETL
                     EDIGPYIDYFFGADVLVFDTQYTFEESLQKIDWGHSSAAIATDIAIKSEVKKLVLYHH
                     DPSYDDAKMDNVLMQAIKYKSMMAPNHPLQIVAAYEGLEIEL"
     gene            2862..3533
                     /locus_tag="PLF85_11825"
     CDS             2862..3533
                     /locus_tag="PLF85_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNE20402.1"
                     /translation="MILASREKWRMGRSVGIQYACCSHLELYPMEELGRQIVITFIPI
                     FVAMDALGILPIFVGMTRKRRENERRRIVALSMLTAFFLTALFTIMGKSIFRFLGITV
                     FDFMVAGGVILFILAILDVMGARHAHDDESGTMGVVPLGTPMIAGPALITTCVLMANE
                     YGIMATLISLSLNIIIAGLLFLQSQVLVRMIGDAGSAALSKITSLLLASIAVMMIRRG
                     VLSMV"
     assembly_gap    3591..3690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3691..4097
                     /locus_tag="PLF85_11830"
     CDS             <3691..4097
                     /locus_tag="PLF85_11830"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HNE20403.1"
                     /translation="VGESWQAGSVVLAWDHVIAGVTNYQDGHNVTFHEFAHQLDQLDG
                     SADGAPPLASWSAAVIWARVFTSEYEKLNAELRSGRKSIIDSYGATHPAEFFAVATEA
                     FFEKPRQLSQKHPALYNELKQYYAVDPLEWLE"
     gene            complement(4106..4534)
                     /locus_tag="PLF85_11835"
     CDS             complement(4106..4534)
                     /locus_tag="PLF85_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20404.1"
                     /translation="MIAAVFEILTVGLPFCAFKLIAGIALSQDWLTAWGIADTLINTI
                     NLVWLLTARRRVTEVCLLSLLVRLLRRPAREHMARWQDLGAAGDMFLSFAIVAFMLGG
                     GFLPGLPAWHLKAWNLSVILNVLGAGSMRLSQSLADLRKQ"
     gene            complement(4538..>5410)
                     /gene="ispG"
                     /locus_tag="PLF85_11840"
                     /gene_synonym="gcpE"
     CDS             complement(4538..>5410)
                     /gene="ispG"
                     /locus_tag="PLF85_11840"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNE20405.1"
                     /translation="KKIKENIHIPLVADIHFQHKLALIAIKSGVDKIRINPGNIGNKD
                     KVSEVLRACKDNGIPIRIGVNAGSLEKDILRKHGYPTSDGMVESALRHIEICNEHNFE
                     DVIVSLKSSDVFMMMEAYRKLAKITDYPLHLGVTEAGTSWQGTIKSSIGIGGLLAEGI
                     GDTIRVSLTTDGTEEIKVGREILRSLGLTSFGVTLVSCPTCGRLEVDLFRIAKEVEEA
                     VAHIKTPLKVAVMGCLVNGPGESKEADIGISAGAGSAILYVKGESKGKIAEAEIVPRL
                     LEEIKLLEDAMKKA"
BASE COUNT         1112 a         1448 c         1531 g         1219 t
ORIGIN      
        1 gtagtacact tcacgaatgc cgcgcccgcc attatcctgg atggtgaacg cgatatcggg
       61 tcgcgtgata acgtcggcct ctgcgttatg gcgaatcgtc aggtcgttgc gctcgttgac
      121 accgatttcg atgcggtaaa acgcgcgttc agacacgttg ccgccgagat cgaccgcctc
      181 gacggcgaac aggtacttgc cgggcggcac atcgttgaag gtgagttcgg ttgaagaagt
      241 cgtgtcggca aacacgcgcg gtagcttttc ggcgaacgcg taacggtagg tggcaatgcc
      301 actgtcatcg gcggcgttga agcgtagaac gacctcctgc cgcgaatcgg gtataccgtt
      361 gtcgtgcgaa gggctggtaa tgatgggacg gctggggggc accgagtcga cgacgaacgg
      421 gtaatgcgta acgggagaga cattcccggc tttgtcgcgg tacttgatgt gcaggtaata
      481 agctccaccg ttgaggccag gcacggtgat ctgcgtggca cgcgcattca tgttaaagag
      541 atccgggtta gtctccctgg tgtcgtcgat gacccaggca aaactctcga cgcccgagat
      601 gtcatctatc ttgggccagt tgaagatggc gtcgcttgat ttggagacct ggccgatggc
      661 atgcgtgcgc gagaagatac gcaccgggtc agccgtcgta tcggcttcac gcacgcagag
      721 accagggccg cagccgtaga acaggaacgg ccggttaccg aggcgcgcga actcgatagg
      781 cttcgcggca atgccttctt tgctcacgcg gtggtcgaca ccgacgaagc tgttgtcggg
      841 ccgatcgaga aatttcgcgt agatctgcgg ggctccgttg cgtgtatcct gccaggcggc
      901 aaccacggta tcgccgcctg cgacaattgc gggcaggtaa cttggcgaag cattgtcgtt
      961 cacggcgatt ggctcgctgc cttcgggcgc cgcaaagata gaccacacct tgttagggtt
     1021 actctgccag ataaaaacga gtttgtcttt tgtatcggtg acttgtgccg agaagttctg
     1081 gaagccgttg ctggtcacgc gcacattgcc cccccagctc acgccaccgt cctggctaaa
     1141 tgcgcgaaag atatcgtcga tgagcgttgc ctcggcgcgg ttctggtaaa atatgtcgat
     1201 gcgtttaccg cgacgaattg ttgccggaaa caatgccccg cgtgtgccgc cgctgatctc
     1261 ggccaccggg ctcagaccag aaaaaactcc ccggccgccg tacgccgcaa ccaactggaa
     1321 gcggtttacc gcagcaggtt cctggtagag gatcacgtgc tggccaggcc ccgtcatttt
     1381 cgcgagcggc agaatcgcgt tgagtttacc cgcagttgaa ggcagcacct gcttggtggt
     1441 aaatcccgtt ggccccgacg cactgtaagt gaagtagata cccgaagcgt taccctgtgg
     1501 cgaagtctcc tgccatgcaa cgagtatctc gtcgcccaat gcagacaccg acgggtgcag
     1561 cggaaaaagc tgatcggcca cagcaagagt gacctctgtc agtacgcggc gttcaaactt
     1621 accgccttct gaatagacca gcgcaagctc gacagttgtg ggagcttctt tgttgaaagc
     1681 gcctttgggc gatgtggtga ctgcatacag tcgcgcttcg ccttcatggc agcagacgcc
     1741 gaactcttcg gcgcggcggt cgacgagcga agggtcgccc cagccgacgg gttcggcaaa
     1801 gagcgtaccc gcggccagaa caagaaggat acgccccttc gacaagctca gggcagcgag
     1861 tatccttctt gtcatagttc gatttctagt ccctcgtatg ctgcaacgat ctgcagcggg
     1921 tggttgggag ccatcatgct cttgtacttg atcgcctgca tcagcacgtt atccattttt
     1981 gcgtcgtcgt aagaagggtc gtggtggtag agtaccagtt tcttcacttc gcttttgatc
     2041 gcgatatccg tagcaatcgc cgctgacgaa tggccccagt cgattttctg cagtgattct
     2101 tcgaaggtgt actgcgtatc gaatacgagc acgtcggcgc cgaagaaata gtcgatgtaa
     2161 ggaccgatgt cttcgagtgt ctcgaggtta aactctgcgt cagaacaaaa gataaaagtt
     2221 ttgccgttgg gctgcctgaa cttgtacgaa taacatccgc ccgggtgacg cacggccttc
     2281 gccgagaccg tgaggccgta gacgtcgaca ctttcggtct cagcaaaatg gtggaacgat
     2341 ttttcgcagt tgagctcgtc aaaggtcagc gggaagtgtg tcgtgatcat ctggtagcgt
     2401 aggcgcgctt cgatgtcagg aaagcacgag tagaagttga gcttgttgcc cgggataaag
     2461 accggcacaa aaaacatgag gccctggatg tggtcccagt gcgagtgggt gaaaaaaaat
     2521 gccatgtcgt ctttgccggt cattttgccg cgcgccatga gtgcgttgcc cagtgccctg
     2581 aggcccgtac ccgcatcgat cacgatcgtc ttgttatcgc tggtctgaat ttctacgcag
     2641 gtggtattgc cgccgtaagt gcctctgatc gaaagaggca ggttactgat aaaattctcg
     2701 atagcttctt tggacagcag gtcggccggg cttgccagct ctagcaccct gcgtatctta
     2761 tcttcgacct ggcgggcggt gattgcggtc gccagagatc cacgcacccc ccaaaaccgg
     2821 ataaacatca accccaaatc aatagccgcg ctgccttagc aatgatttta gcatcgcgcg
     2881 agaaatggcg catgggccgt tcggtcggaa ttcagtatgc ctgctgcagc cacctcgaac
     2941 tttaccccat ggaagagctt ggccgacaaa ttgttatcac gtttattccc attttcgtcg
     3001 cgatggacgc cctcggtatt ctgcccattt tcgttggcat gacccgcaag cgccgcgaaa
     3061 atgagcgccg acgtatcgtc gcgctttcga tgctgacagc ctttttcctg acggcgcttt
     3121 ttaccatcat gggcaagtcg attttccggt tcctgggcat caccgtgttc gactttatgg
     3181 tggcaggggg agtgatcctc tttatcctgg caattctcga tgtcatgggt gcacgccatg
     3241 cgcacgacga tgagtctggc accatggggg ttgttccgct cggtacgccg atgattgcag
     3301 gcccggcgct gatcactacc tgcgtgctga tggcgaacga atacggcatc atggcgaccc
     3361 tcatttcgct ttcgctcaac atcatcatcg ccggtttatt gttcctgcag tcgcaggtcc
     3421 tggtgcgcat gataggagac gcaggatctg ccgcgctgtc aaagattacc agtttgctgc
     3481 tggcatctat tgccgtgatg atgatcaggc gtggcgtgct gagtatggtt tagacggtct
     3541 gcaatcggtg gagtatttct tctacgtctt tgccggagtt attgccggct nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgtcggtga atcctggcag gcaggtagcg
     3721 tcgtgctcgc ctgggatcac gtcattgccg gggtcacgaa ctaccaggac gggcacaatg
     3781 tcacgttcca tgagttcgcg caccagctcg accagctcga tggcagtgcc gatggcgctc
     3841 cgccgcttgc atcctggtcg gctgcagtaa tctgggcaag ggtgttcacc agtgaatacg
     3901 agaaactcaa cgcagaactg cgcagcggta gaaagagtat tatcgactca tacggtgcga
     3961 cgcacccggc ggaatttttt gccgttgcga cggaggcatt ctttgagaaa cctcgccagc
     4021 tctcgcagaa gcatcccgct ctttacaacg aactgaagca gtattacgcc gtagatccgc
     4081 tcgaatggct ggagtagaag agtaatcact gcttgcgcag gtctgccagc gactgtgaca
     4141 accgcatcga ccctgcgcct aacacgttca gtatcacaga aagattccag gcttttaggt
     4201 gccatgccgg taacccgggc agaaatccgc cgccgagcat gaaggcaaca atcgcaaatg
     4261 acaggaacat atcgccagct gcaccgagat cctgccaccg cgccatgtgc tctctggctg
     4321 gacggcgtag gagccgcacc agtagcgaaa gcaggcagac ctcggtaacc cggcggcggg
     4381 ccgtgagcaa ccagaccaaa ttgatcgtgt tgatgagcgt gtcggcaatg ccccacgctg
     4441 tgagccagtc ctgcgaaagg gcaataccgg cgatgagctt gaaggcacaa aacggcagtc
     4501 cgaccgtaag tatctcgaag actgcagcga tcatctatca ggcttttttc attgcgtctt
     4561 cgagcagctt tatctcttcg agcaggcgcg gtacaatttc tgcctctgcg atcttgccct
     4621 tgctttcgcc tttcacgtag aggatggctg aaccagcacc tgcagaaata ccgatgtcgg
     4681 cttctttcga ttcgccaggg ccgtttacca ggcaacccat taccgcgacc ttcagtggag
     4741 tcttgatgtg cgcgacagct tcttcaacct ctttcgcaat acggaaaaga tccacttcga
     4801 gacggccaca ggttgggcac gatacgagag tgacgccgaa actggttaag cccagtgacc
     4861 gcagaatctc ccgcccaacc ttaatctctt cggtgccgtc ggtcgtgagc gagacgcgaa
     4921 ttgtgtcgcc gatgccctcg gcgagcaggc cgccgatacc gatcgacgac ttgatcgtgc
     4981 cctgccagct cgtgcccgct tcagtcacgc cgaggtgcag agggtaatcg gtgatcttgg
     5041 cgagcttgcg gtatgcctcc atcatcatga aaacatcgct cgacttgagt gaaacgatca
     5101 catcttcgaa attgtgttca ttgcagatct cgatgtggcg gagcgctgat tctaccatac
     5161 catcagacgt cgggtaacca tgcttgcgca ggatatcttt ttcgagtgat cccgcattga
     5221 cgccaatacg aatcggaata ccgttatctt tgcaggcgcg cagtacttcg gataccttgt
     5281 ctttgttacc gatattgccc gggttgatgc gtatcttgtc gacgccgctt ttgatggcga
     5341 tcagcgcaag tttgtgctgg aaatggatgt cggcgacgag ggggatgtga atgttttctt
     5401 tgatcttttt
//
LOCUS       DAOQJF010000230         5407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8767_length_5407_cov_5.742339, whole genome shotgun sequence.
ACCESSION   DAOQJF010000230 DAOQJF010000000
VERSION     DAOQJF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8767_length_5407_cov_5.742339"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLF85_11845"
     CDS             <1..732
                     /locus_tag="PLF85_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE20406.1"
                     /translation="GECYKAVRTALEVGYRHIDTARIYGNEEDVGRAIRDSGIAREEI
                     YVTTKLWNSDQPKAQKAFDDSLARLGLDYIDLYLVHFPVTKSRAQAWKDMEHILASGK
                     TRSVGVSNYMQRHLDELLAASSLKPVVNQVELHPWLSQQELKQHCESRGIRIEAYSPL
                     AHGQKVGDLSLKPMADKYGKSVAQLILRWSIQKGNIVLVKSVSPQRIAENFAIFDFEI
                     SVQDMAKLDALDENLRTCWDPSNTP"
     gene            complement(816..2153)
                     /locus_tag="PLF85_11850"
     CDS             complement(816..2153)
                     /locus_tag="PLF85_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE20407.1"
                     /translation="MARLVRQLGFVTAAFTVIGSMIGSGVFRTGNELLFDAKIAPQAI
                     LALWAIGGLVALAGALSYAELAAAYPEAGGEFAYLNRIFGKLPAFLTGFISLTVGFSA
                     PIAFTSLVIIELVVGGINQLQAEQVSVSAFKLQLLAAFIPVLLAFFHIARVKLGEGFQ
                     NVLTAVKTLLIIVIAVALFFVSAPASTPILPQATTPEFWKWGSLLLTVMFAYSGWNAA
                     SYLGAEIKDPIKNMPRAMVIGTLLTAVIYLLLNLNFLRFAGLNRETTDLALGAFTALL
                     GKAGGAALSFMVAFFLVSSVSAEVMIGPRVYYAMAEQGLLFRKLAQVQPKLGTPAFAI
                     LLQTAISVVYILAFGFGGTLKLLIFMGFALSFFPWLTVFGLVYASYVHKTGIIANRPY
                     RSPLFPLFPLVYLAASLFMMLSSLIYSTEQSLWAIGITGVGAVSFYFWQKLKK"
     gene            complement(2155..3336)
                     /locus_tag="PLF85_11855"
     CDS             complement(2155..3336)
                     /locus_tag="PLF85_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20408.1"
                     /translation="MKKLLLPALALAALLAACQSSKVVDEKTGRAAGAQAEGTTGYYY
                     DDMARYLAGFDLPGDSNLMPYTQGPEYTEHRQKMARFWERVRAQSIEPITEWRESTLN
                     RNPNGPVCRRDRAAIYPLCGADIINLYTICPQASEYVMIALEKAGDIPHPEKQTASYF
                     QGLSRMRSVINNIADRNYFFSAHMKTNIIKNEEVPGIAPVLLAFTSGLGWRIVNMEKI
                     YLAPDGRPVALDDKNLDFRPRGVRIWFRTADDPRLRSLTYIEQYLDSASVAPDYPLTK
                     FLNEKKGSMMMLKAAVYLMHNNYYRSVRERFMAIPDVIVQDDSGFRYVDLKPNFDISI
                     YGDFVGPAIRMSVIPKNPVQPELAALYAERKPPTLPFHFGYGVLRKPPRSNLMVAHRK
                     N"
     gene            complement(3352..4470)
                     /gene="prfB"
                     /locus_tag="PLF85_11860"
     CDS             complement(3352..4470)
                     /gene="prfB"
                     /locus_tag="PLF85_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNE20409.1"
                     /translation="MAQSIKEVKNELGGLKSQIEQRYTELNLQDVEAELGDLRQKMED
                     PALFADDSKVNQVRELQTRIAFLERKTSAWNAIKHEINDALEYAEIAEMEGAENMADE
                     LLQRGNAVRVEFDKLDLESLLNGPDDMNNAFVNIHPGAGGTESQDWADMLLRAYTRYA
                     EKMGFEVEMVDYQEGEEAGIKNATLFVKGPNAFGYLKAENGIHRLVRISPFDSNKRRH
                     TSFAAVHVSPEISDDVEVEVLEKDLRVDVYRASGAGGQHVNKTESAVRMTHLPTGIVV
                     SSQTQRSQIQNRLTCMKMLKAQLYAKARAEKEAEIESRSGERKDISWGNQIRSYVFHP
                     YNMVKDLRTGHETAQVQDVMDGEFAPFIDAYLRQLKKN"
     gene            4584..5405
                     /locus_tag="PLF85_11865"
     CDS             4584..5405
                     /locus_tag="PLF85_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20410.1"
                     /translation="MPHADGQLIGKYRSLLLPAEQLAPEASLLQHDLQSLAQQHQEGR
                     ISDALYATLFVLLFAFRRALGPEWLQHHRYTYWTHVVQGVSGGVIPAETEIALKLRKL
                     PLRGMPGFVFVTLADWLERRIDLHLARSPVSPEQMLAVQAAGSRFVTVALSDALAGKL
                     IDNKRDAFEFCLHDIGHAYTFFRVEHDPAGQLRFFAQLQNDLAQLDGLSSADAKFAAD
                     LEYCMSDMNSHPEHLRQYLRGVIVEAFYRLRKNDTQGQYSEEELSRFLAGLGAVV"
BASE COUNT         1167 a         1508 c         1571 g         1161 t
ORIGIN      
        1 ggcgaatgtt acaaagccgt gcgcacagcg ctcgaagtcg gctaccgtca tatcgacacg
       61 gcgcgcatct atggtaacga agaagacgtt ggccgtgcga ttcgcgacag cggcattgcg
      121 cgtgaagaaa tctacgtcac aaccaaactt tggaacagcg accagcccaa agcgcagaag
      181 gcatttgacg acagccttgc ccgactcggt ttagattaca tcgatctcta cctggtgcac
      241 tttccggtga cgaaaagccg cgcgcaggcg tggaaagaca tggaacacat tcttgcgtcg
      301 ggcaaaaccc gttcggtggg ggtgagcaac tatatgcagc gccacctcga cgaactgctg
      361 gccgcgtctt cgctcaagcc agtcgtcaac caggtcgagc tacacccgtg gctcagccag
      421 caagagctca agcagcactg cgaatcacgc ggcattcgta tcgaagccta cagccccttg
      481 gcgcatggtc aaaaggtggg ggatttatcc ctcaaaccga tggctgataa gtatggcaaa
      541 tctgtcgcgc agctgatact gcgctggtcg atccagaagg gaaacattgt tttggtaaaa
      601 tcggtttcac cgcagcgcat tgcagagaac ttcgcgatct ttgattttga aatcagtgtg
      661 caggatatgg cgaagctcga cgcgctcgat gaaaacctgc gtacctgttg ggatccttcg
      721 aacactcctt gagaaccctc accccccaac cccctctccc tgttagggcg aggggggcca
      781 gtaccctccc ctaacagggg agggcagggt ggggtttact tcttaagctt ctgccagaaa
      841 taaaaactca ccgcaccgac accagtgatg ccgattgccc acagtgactg ctcggtgctg
      901 tagatcagtg atgacagcat catgaagagc gaagcggcca ggtagaccag cggaaaaagt
      961 ggaaataagg gcgagcggta cgggcggttg gcgatgatgc ccgtcttgtg tacataggaa
     1021 gcgtagacca ggccgaacac tgtcagccac ggaaagaaac tcagcgcaaa acccatgaag
     1081 atcagcagct tgagtgtacc gccaaaacca aatgcgagaa tatagacgac gctgatggcg
     1141 gtctgcagca gaatcgcaaa agccggcgta ccgagcttcg gctgtacctg agcgagcttg
     1201 cggaacagca ggccctgttc ggccatcgcg tagtagacac gcggcccgat catgacttca
     1261 gccgatacag aagagacaag aaagaaggct accatgaaag acagcgcggc accaccggct
     1321 ttgccgagca gcgcagtgaa agcgccgagc gccaggtctg tggtctcgcg gttgagcccc
     1381 gcgaagcgca ggaagttcaa attgagtaag aggtaaatta ccgcggtcaa caatgtacct
     1441 atgaccatcg cgcgcggcat gttcttgatc gggtctttga tctctgcgcc gaggtaagat
     1501 gccgcgttcc agccgctgta agcaaacatg accgtcagca gcaggctgcc ccatttccag
     1561 aattcgggtg ttgttgcctg cggcagtatg ggggtcgaag ccggagcgga gacgaagaac
     1621 agcgcgacgg caatgacgat aatcagcagc gttttgaccg cggtcagaac gttctgaaat
     1681 ccctcgccga gtttgacgcg cgcaatgtgg aagaaagcca gcagaacggg aataaacgcc
     1741 gccagcagct gtagtttgaa cgctgaaaca gaaacctgct ctgcctgcag ctggttgata
     1801 ccgccaacca ccagctcgat aatgaccagc gatgtgaagg cgatcggcgc agaaaaacct
     1861 accgtcagcg agatgaaacc ggtgagaaaa gccggcagct tgccaaagat acgattgagg
     1921 taggcaaatt cgccgcccgc ttcggggtaa gctgcagcca gttctgcgta gctcagcgca
     1981 cccgcgagcg cgacaagccc gccgatcgcc cagagtgcga gaattgcctg cggagctatc
     2041 ttcgcgtcaa acagcagctc gttgcccgtg cgaaagacgc ccgagccgat catgctgccg
     2101 atcaccgtga aagccgcggt gacgaaaccg agttgccgta cgagtcttgc catgtcagtt
     2161 cttcctgtgc gcgaccatca ggttcgagcg cggcggtttg cggagcacgc cataaccaaa
     2221 gtggaacggg agcgtcggcg gtttgcgctc tgcatacaat gcggcgagtt cgggttgtac
     2281 cgggttcttt ggtattaccg acatacgaat cgccgggccg acaaagtcgc catagatcga
     2341 aatgtcgaag ttaggcttca gatcaacgta acggaagcct gaatcatcct gcacgatcac
     2401 atcgggaatc gccatgaacc gctcgcgtac cgaccggtag tagttattgt gcatgagata
     2461 gactgcagcc ttgagcatca tcatgctgcc ctttttttca ttgagaaatt tggtcagggg
     2521 gtaatcgggt gccacacttg ccgagtcgag gtattgctcg atgtaggtca gcgagcgcag
     2581 cctagggtca tctgccgtgc gaaaccagat gcgcacaccg cgcggcctga aatccagatt
     2641 cttgtcgtcg agcgccacgg gccgcccgtc gggggccagg tagatcttct ccatgttcac
     2701 gatgcgccag ccaagccctg aagtgaatgc aagcagtacc ggcgcaatac caggaacttc
     2761 ttcattcttg atgatgtttg tcttcatgtg tgcagagaag aaatagttgc ggtcggcaat
     2821 gttgttgatg accgaccgca tgcgcgagag cccctggaag taactcgctg tctgcttttc
     2881 agggtgcgga atgtcgccgg ctttttcgag tgcaatcatc acgtattcgc ttgcctgcgg
     2941 gcaaatggta tagaggttga tgatgtccgc gccgcatagc ggataaatag ctgcacggtc
     3001 tctgcggcaa accgggccat taggattacg gttgagtgtc gattcgcgcc attcggtaat
     3061 gggttcaata ctctgcgcac gcacacgctc ccaaaagcgc gccatcttct gccggtgttc
     3121 agtgtactca gggccctgcg tatacggcat caggttgctg tcgcccggca ggtcaaagcc
     3181 tgcgaggtac cgcgccatat cgtcgtagta gtagcctgta gtgccttcgg cctgtgctcc
     3241 ggcggcgcgg ccggttttct cgtcgacgac tttagaactt tggcaggccg cgagtaacgc
     3301 tgcgagcgcg agagccggca atagcagttt tttcatgggg agaaccttgg tttagttttt
     3361 tttcagctgt ctgagatatg catcgataaa gggagcaaat tcgccatcca taacatcctg
     3421 cacctgcgcg gtttcgtgac ccgtgcgcaa atctttcacc atgttataag gatggaaaac
     3481 ataactgcga atctggttac cccacgagat gtctttgcgc tcgccgctgc gtgattctat
     3541 ctctgcttct ttctcggcgc gcgccttggc gtagagctgt gccttgagca tcttcataca
     3601 agtcaggcgg ttctgtatct ggctgcgctg cgtctggctc gagaccacga tacctgtggg
     3661 taagtgcgtc atgcgcacgg cagattcggt tttgttgacg tgctggccac cggcgccgct
     3721 cgcacggtag acgtcgacgc gcagatcttt ctctagtact tcaacttcaa cgtcgtcaga
     3781 gatttcgggt gaaacatgca ccgccgcgaa agaggtgtgg cggcgcttgt ttgagtcaaa
     3841 cggcgatatg cgcacgaggc ggtggatgcc gttctcggcc ttgagataac cgaatgcatt
     3901 ggggcccttc acaaacagcg ttgcattctt gatgcccgct tcttcgcctt cctggtaatc
     3961 gaccatctcg acttcaaagc ccattttttc tgcgtagcgc gtgtaagcac gcagcagcat
     4021 gtcggcccag tcctgcgatt cggtgccgcc cgcgccgggg tgaatgttca cgaacgcgtt
     4081 gttcatgtcg tcggggccgt tgagcaggct ctcgaggtcg agcttgtcga actctacccg
     4141 cacggcattg ccgcgctgca gcagttcgtc ggccatgttc tctgcgcctt ccatttcggc
     4201 gatctcggcg tattcgagcg catcgttgat ttcgtgcttg atagcgttcc aggcgctggt
     4261 cttgcgttcg agaaatgcga tgcgtgtctg cagctcgcgc acctggttca ccttgctgtc
     4321 atcggcgaag agtgccgggt cttccatctt ctggcgcagg tcacccagct cggcctcgac
     4381 atcctgcagg ttcagctcgg tgtagcgctg ctcgatctgg ctcttcaggc cgccgagctc
     4441 gtttttgact tcttttatgg actgcgccac agggggtaaa accggcagcg cagacggcgc
     4501 ggaaagtaaa aacaaccggg agaatccgcg tggtgtgacg cttgcggctt gccgcaccgt
     4561 ctcgcaatgc aaatggctcg cccgtgcccc acgcagacgg ccagctgatc ggcaaatacc
     4621 gcagcctgtt gttgcccgcc gaacagctgg cgcctgaagc cagtttgctg cagcacgacc
     4681 tgcagtccct cgcgcagcaa caccaagagg gcagaatttc cgatgccctt tatgcgacgc
     4741 tctttgttct gctttttgcc ttccggcgtg ccctcgggcc tgaatggtta cagcaccacc
     4801 gctacaccta ctggacacac gtggtgcaag gtgtttcagg gggagttatc cctgcggaaa
     4861 cggaaattgc cctaaaactg agaaagctcc ccctgcgtgg catgcccggt ttcgtgtttg
     4921 tgactttggc cgattggctc gaacgccgca tcgacctgca ccttgcccgc tcacccgttt
     4981 cgccagagca gatgctggcc gtacaggcag caggctcacg ttttgtcacg gtcgcgctca
     5041 gcgacgccct tgcaggaaaa ctgatcgata acaagcgcga cgcgtttgaa ttctgtctgc
     5101 acgacattgg gcacgcgtat actttttttc gcgtcgaaca cgacccggcc gggcagttgc
     5161 gcttcttcgc gcagctgcag aatgacctcg cgcagttgga cggcctgtcg tctgcagacg
     5221 ccaagttcgc cgccgatctc gagtactgca tgagcgacat gaacagccat cccgagcacc
     5281 tgcggcaata cctgcgtggc gtcatcgtcg aggcgtttta ccggctgcga aagaacgata
     5341 cgcaggggca gtatagtgaa gaggagttga gtcggttttt ggcggggctg ggggcagtgg
     5401 tgtaggt
//
LOCUS       DAOQJF010000231         5393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8807_length_5393_cov_6.064631, whole genome shotgun sequence.
ACCESSION   DAOQJF010000231 DAOQJF010000000
VERSION     DAOQJF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8807_length_5393_cov_6.064631"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLF85_11870"
     CDS             complement(<1..198)
                     /locus_tag="PLF85_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20411.1"
                     /translation="MTIQAALASKIVFSWYDKFDLAKSLYGNEAGIENRLTTPDVSLC
                     PITPGLLCGELQSPQQPQAVSP"
     gene            340..945
                     /locus_tag="PLF85_11875"
     CDS             340..945
                     /locus_tag="PLF85_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20412.1"
                     /translation="MDPMMISSVLHINREYRLSWQLEKLRAHLLANPPRGRRPMLRFA
                     EPGFAASESEVKLLKGIDRITASALYCDIYPLPWDFYSELETMAARGEYYATHALLAA
                     VILQQKKCSVDRDRLTGIQQGLERTVAKIAGSQQPLNDLRIEAVLMLLLAGGRDAIRA
                     EWIAEIYLAQSPDGSYFRNDHSTVLAGWAMLEYARLQGGER"
     gene            complement(1033..1644)
                     /locus_tag="PLF85_11880"
     CDS             complement(1033..1644)
                     /locus_tag="PLF85_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE20413.1"
                     /translation="MLSLHQFHLQLRRVQPDDLEEIRQGRNTDSVRKNHIYQQLITPD
                     MQNDWFVRTLKPENYYFVIIKRGKKIGLVHVSNIREDLSCADYGLFLWDAAYRGSRVP
                     LLTTLLILDFFFVQVQVTMLTGKVLKSNAAIQRICRFFHFDLTGNPGDEFLTTSSTLQ
                     NYLSHRQEFLSFARRCVHDDNECRLTIRGDASTPHLPAVSRLL"
     gene            1757..3307
                     /locus_tag="PLF85_11885"
     CDS             1757..3307
                     /locus_tag="PLF85_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20414.1"
                     /translation="MLQVVRAHRIFALGAIVYALILAFLPLQRQAMETYAYSAAIEGY
                     YNLSKTFNLYMQSEQIPDISRWHPNHPLPGFFAGIMYDHFGISALNWFRGWNLFWAIG
                     LITVFYRFMLTLKFSNKTSALGALLLGITHGIWTMALSAETHLPALALAVVAADYLVK
                     YLLDGAPGKLLCSAMIFSLATGFHLAAGLLVIPAFLALLSHGWRKEYAGAAAAFIAGF
                     LFFYLLCPVVILRIGSINQFNQTLLMYKHLVHQRYEGLYWFWIAMLTFLRSIIYIDAA
                     QVVSAIVVLILTLYTIWTGARNLIAVRPVRILLIGVPLTYLFSHMVLGARPDGVNGWL
                     FTLPALLALFAAAFAQGRGLRRLVLPVVLSGLFVVNLIGVVLPNALMHSNQVYFFTPP
                     ANLGKETPVGFVIDNPVLTYAEIWNAGTHFGLRNQTVFLPCCGEQNVRDNVAQWLLRN
                     RDRFLLISDQAGEQVPELLRQLGIAFSVTLSQQSLVEQRLVPSSIYTEYPSNGIMTKM
                     LRVYVPAS"
     gene            complement(3403..3867)
                     /locus_tag="PLF85_11890"
     CDS             complement(3403..3867)
                     /locus_tag="PLF85_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HNE20415.1"
                     /translation="MKPLSNPTDFSPALAPVNCPMGSYFRLFFLTLAAAYLVFAAAVQ
                     YNDPDPLHWMALYALTAICCLGAIAEKYWPWLLWMLLGMSVTEMLIALDGFRQWLALG
                     GQNLLSQQMAAEKPWIEQSREFLGAAINLGIVVYLLRRQVRAKGILSENEID"
     gene            3985..4902
                     /locus_tag="PLF85_11895"
     CDS             3985..4902
                     /locus_tag="PLF85_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNE20416.1"
                     /translation="MKSIMVALAAMLSVSQLFAGSGSSTSLNGVYPIVLSHGMFGWGT
                     TDTNSLIGILNYWGGMDDHLRSKGAIVYAPGKSALQSNAVRAQELKDKINLFMAAGGH
                     TKIHIVGHSQGGLDSRYMVTNLGMASKVSTLTTLNTPHKGSPIADIVLGVVPSWLMPY
                     VSTTINGLVKVIWGGSQNNLNASLSSLTISNANSFNAATPDASGVKYFSYGSKVTVPD
                     LIQHPLMGLLVPVTSVGGVAKGQGIENDGLVPLSSQKWGTWKGSPSYSFWVTGLDHLQ
                     VTNTLSSGQAWFDVKSFWLSLAQNAKSNQ"
     gene            complement(4936..>5393)
                     /gene="nth"
                     /locus_tag="PLF85_11900"
     CDS             complement(4936..>5393)
                     /gene="nth"
                     /locus_tag="PLF85_11900"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNE20417.1"
                     /translation="STGFYRNKARNIVALAKILVEKYDGRIPRDFDVLHSLPGIGRKT
                     ANVIMAEAFGEAPGITVDTHVKRISRLLGFTRSDNAIIVERELMGIFPKKTWRDMPLL
                     LIFHGRKTCIARRPKCPECKIKRLCPSAAPIAIRGGAARGTSVSRRAKP"
BASE COUNT         1220 a         1377 c         1439 g         1357 t
ORIGIN      
        1 aggtgaaacg gcctgtggct gctgggggga ttgcagctcg ccacacagca ggccaggagt
       61 aatagggcaa agcgaaacat caggcgttgt gagcctgttc tctatgccag cctcgttgcc
      121 gtaaagactt tttgccaaat cgaatttgtc ataccatgag aagacgattt tgcttgccaa
      181 tgctgcctga atggtcatat ttttcccgtg agcacaagac taggttgctg gttgtttttc
      241 gtctgcttac tgaattcaga cctatttgct gtagctccaa agcagtttcg tctttcaggc
      301 atcagggccg tggactcagt caggaaaaag ggcctgagta tggatccaat gatgatttct
      361 tcggttcttc atatcaacag agagtatcgt ctctcgtggc agcttgaaaa gctacgggca
      421 caccttttgg caaatccgcc ccgtgggcgg cgtccgatgc tccggttcgc agagccgggc
      481 tttgcagcct ccgaaagcga ggtgaagttg ctcaaaggaa ttgaccgtat cacagccagc
      541 gcactctatt gtgatatcta tccgttgcca tgggattttt atagtgagct cgagactatg
      601 gcagcgcggg gtgagtacta tgcaactcat gctctgctcg ctgccgtcat cttgcagcaa
      661 aagaaatgtt ccgttgatag ggaccgcctg accggaattc agcagggcct ggaaagaacc
      721 gttgccaaaa ttgcgggtag tcaacagccg ctaaatgatt taagaatcga agctgtgcta
      781 atgctcctgc tggcgggagg cagggatgcg atcagggcgg aatggattgc agaaatctac
      841 cttgcacagt caccagacgg cagctatttt cgcaatgatc atagtactgt gcttgcaggc
      901 tgggccatgc ttgaatacgc caggcttcag gggggcgaac gctagcccct ggaggcctgc
      961 cttaactatc cggccaacga aaagtgcgtg gtcaaataag ccagaccaag gccccaatga
     1021 ttctttagtt catcacagta atcgggaaac ggccggtaga tggggcgtcg aggcgtcacc
     1081 cctgattgtg aggcggcatt cgttatcgtc atggacacac ctgcgggcaa aactcaggaa
     1141 ctcctgcctg tgtgacagat agttctgcag ggtcgatgaa gtcgtcagaa attcatcacc
     1201 agggttaccc gttagatcaa aatgaaagaa gcggcagatg cgctgtatcg cagcgttcga
     1261 cttcagtacc tttcctgtta gcatcgtgac ttgtacctgg acgaagaaaa aatccaggat
     1321 taataaggtg gtcaataatg gcacacgcga cccccggtat gcggcatccc agagaaagag
     1381 cccatagtct gcacaggaga ggtcctcccg gatattgctt acatgaacca gaccaatttt
     1441 ctttcccctc tttattatga cgaaatagta attttctggt ttgagagttc tgacaaacca
     1501 gtcgttttgc atatctggtg tgataagttg ctggtagatg tgattcttgc gcaccgagtc
     1561 ggtgtttcgt ccctgtcgga tttcttccag atcatcaggc tgcaccctgc gcagctggag
     1621 gtgaaactgg tgcagactca gcattctgac taattggcac ggtcgtggcc tcacaatgta
     1681 aagaaaaacc ccggcataaa gaaaacgcac gcgacggcat tgattgacgc accgttcgcg
     1741 ggttcatcat tagggtgtgc tgcaggttgt ccgtgctcac cgtatatttg ccctcggagc
     1801 aattgtctat gcgctaatcc tcgctttttt gccgttgcag cgccaggcaa tggagaccta
     1861 tgcgtacagc gcggccatag aggggtacta taacctgagt aagaccttca acttgtatat
     1921 gcaatctgaa cagataccag atatttcgcg atggcatcct aatcatcctt taccaggctt
     1981 ctttgcaggg ataatgtatg atcatttcgg aatttctgcc ctgaactggt ttcggggctg
     2041 gaatctcttc tgggcaatag gactcatcac ggtattctac cgctttatgc tcacgctcaa
     2101 attcagcaat aaaaccagcg cgctgggcgc actcctgctg gggattactc atggtatttg
     2161 gacaatggca ctttctgccg aaacacacct gccagccctt gcactggcag ttgttgccgc
     2221 ggactatctg gtgaaatatc tgctggacgg tgcgcctgga aaactgttgt gcagcgcgat
     2281 gatattcagc cttgctacgg ggtttcatct tgccgccggc ttgcttgtga ttccggcatt
     2341 tttggccttg ctctcgcacg gctggcgaaa ggaatatgct ggagcggcag cagctttcat
     2401 agcaggtttc ctttttttct acttgctctg tccggtcgtc atactgcgca tcgggagtat
     2461 caatcagttc aaccagactc tcctcatgta caaacacctt gtgcatcaga gatacgaggg
     2521 attgtactgg ttctggatcg ctatgctcac attcctgcgt tcgatcattt acattgatgc
     2581 tgcgcaagta gtttcagcaa ttgttgtgct gatcctgacc ctctatacta tatggactgg
     2641 agcgcgaaac ctgatcgctg tacgacctgt ccgtatactg cttatagggg tgcctctgac
     2701 ctatctcttt tcgcatatgg tacttggggc caggccggac ggtgtcaatg gttggctttt
     2761 cacattgccg gcgctcctgg cgttgtttgc cgcggcattc gcgcaggggc gcgggctgag
     2821 acgcctggtg ctgcccgttg ttttgtccgg tctttttgtg gtgaatctca ttggcgtagt
     2881 gttgccaaac gcgctcatgc attctaatca ggtgtacttt tttacaccgc cggctaacct
     2941 cgggaaagaa acgcccgttg gttttgtgat cgataacccg gttttgactt atgcagaaat
     3001 ctggaatgct ggtacgcact ttggcttaag aaaccagact gtatttctcc catgttgcgg
     3061 tgaacagaat gtacgggata acgttgcgca atggttactg cgcaaccggg accgttttct
     3121 gctgatttct gaccaggccg gagaacaagt acctgaacta ctcaggcagc tgggtattgc
     3181 attttcggtt acactgtcgc agcagtcatt agttgagcaa agattggtgc caagctctat
     3241 ttacaccgaa tatccatcaa acggcataat gaccaaaatg ctgcgcgtct atgtcccggc
     3301 ttcctgagta agaaacctgt ggaaattgag ctggattgca ggactctcgc cggacttcgt
     3361 cggcgtattc aattctaaaa cgggtgaggt ttgttttgca cgttaatcta tttcgttttc
     3421 gcttaaaatc ccctttgccc gaacctgccg gcgcaacaag tacaccacga tccccaggtt
     3481 gatcgcggcg cctaagaatt cgcggctctg ttcgatccag ggtttttctg cagccatctg
     3541 ctgggacagc agattctgac cgcccagcgc aagccactgc cgaaagccgt cgagagcgat
     3601 cagcatttct gtcacactca tccccagcag catccagagt agccagggcc agtacttttc
     3661 tgcgatggcg cccaggcagc agatggccgt cagcgcgtag agggccatcc agtgcagggg
     3721 gtcaggatcg ttatactgca cggcggcggc aaagacgagg taagcggcgg ccagggttaa
     3781 gaaaaacagg cgaaaatagc tgcccatcgg gcaatttaca ggggcgagcg cgggcgaaaa
     3841 gtccgtcggg ttgctcaagg gtttcatgga cactccgttc atttttttgg cactgcacgc
     3901 taaaataatg acatatatag cagatactgt agcgcttgct atatgcggca gtgtccaata
     3961 acacgtgaag gggatcttat cgaaatgaaa tccattatgg tggctcttgc agccatgtta
     4021 tccgtctctc agctttttgc cggttctggc agcagcacca gcctgaacgg ggtatacccc
     4081 atcgtgcttt cgcacggcat gtttggttgg ggcacaactg ataccaactc attgatcggt
     4141 atcctgaatt actggggtgg aatggatgac cacctgcgtt caaagggagc gattgtttat
     4201 gctcccggca agtcggccct gcagtcgaac gccgtgcgcg cacaagaact caaagataag
     4261 atcaacctct tcatggccgc tggtggccat accaagatcc atatcgtcgg gcactcgcag
     4321 ggtggcctcg acagccgcta catggtaact aacctgggta tggcgagcaa agtctcgacg
     4381 ttgactacgc tgaatactcc gcacaagggc agtccaatcg ccgacatcgt gcttggcgtt
     4441 gtgccttcgt ggctgatgcc gtatgtcagc acaacgatca acggccttgt caaagtcatc
     4501 tggggcggat ctcagaacaa cctgaacgcg tcgctcagct cgctgactat ctcaaacgcc
     4561 aacagcttta atgccgccac gccagacgcg agcggcgtga aatacttctc ttacggttca
     4621 aaggttaccg tgcctgatct gatccagcat cctctcatgg gcctgctggt gcctgtcact
     4681 agtgtcggtg gtgtggcaaa aggccagggc attgaaaacg atggtctcgt accgcttagc
     4741 tcacagaaat ggggcacctg gaagggtagc ccgagctaca gcttctgggt aacaggtctg
     4801 gatcacctgc aggttactaa caccctgtcc tcaggccagg catggtttga tgtgaagagt
     4861 ttttggcttt cactcgccca gaacgcgaaa agtaaccagt aatttcagga cgccggcaaa
     4921 acggcggctg gaaaatcaag gctttgccct acgggacact gatgtcccgc gggctgctcc
     4981 gccacggatg gcaattgggg cagccgacgg gcaaagcctt tttattttgc actccgggca
     5041 tttcggccgc cgggcgatac aggtcttgcg cccgtgaaaa atcagcagta ggggcatatc
     5101 gcgccacgtt ttcttcggga atattcccat cagttcgcgt tcgacgatga ttgcgttgtc
     5161 actcctggtg aagccgagca gccgcgaaat gcgcttcaca tgggtatcga cagtaatacc
     5221 cggagcttca ccgaatgcct cggccatgat cacattggct gtcttacgcc cgatacccgg
     5281 cagtgagtgc agcacgtcaa aatcacgcgg aatacggcca tcgtactttt cgacgagtat
     5341 ctttgccagt gctacaatgt tcctggcttt gttgcggtag aaacccgtgc tgt
//
LOCUS       DAOQJF010000232         5358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8887_length_5358_cov_5.155195, whole genome shotgun sequence.
ACCESSION   DAOQJF010000232 DAOQJF010000000
VERSION     DAOQJF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8887_length_5358_cov_5.155195"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1543
                     /locus_tag="PLF85_11905"
     CDS             116..1543
                     /locus_tag="PLF85_11905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE20418.1"
                     /translation="MKKTFYELGLSPTLVQAVTDMGFREASPIQEKAIPILLEGHDLI
                     GQAQTGTGKTAAFAIPVIEKITAAKELQAMVLCPTRELAMQVAAEFKKLLKYKKEIQV
                     VTVYGGQPIGAQFRDLKEKPQIVVGTPGRVMDHMRRKTIKMNTVKFFVLDEADEMLAQ
                     GFREDIEHILTDAPEDRQTVLFSATMSKEILAITSQFLNNPQRADVLAGASNKPKIEQ
                     FFLIVPEKNRVEATIRLMDFYGFKSALIFSNMRSQVDSLTQQFNERGMTAEGIHGDLN
                     QNQRDKVMARLRSGHARLLVATDVAGRGIDISHIEAVFNFDLPRDVEDYVHRIGRTGR
                     AGKSGCSFSFVRPTELGKLRRLEKLNNIQILPHDVPAAEDLQGKKVSTLLAELVDITK
                     RAKLAQYLEYLHDLTSEEISPEEIAAAAIKMLLEKDGRQYDNKVQFTTAKDELKKAPG
                     DKHRPRNYPPRSGKKFYRNKRNKKN"
     gene            complement(1548..2120)
                     /locus_tag="PLF85_11910"
     CDS             complement(1548..2120)
                     /locus_tag="PLF85_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20419.1"
                     /translation="MYLRNIGFLAAFVFARCSSVEYRYVPEKPQMYSKTIGGKPVNAD
                     LNPTYFDLEDAGKIRSYFIFSESQDVATNLESLAVDLADEKDGTRIPATKSDLVVSYW
                     NGKSIVEIRKNANTESLSLLLPPELQQSPFRGSHVVTYSYHFEYASLALPDILTQTIT
                     IRFKDRTVTMKTRLHKRKFEKAFISGRPFG"
     gene            complement(2141..3739)
                     /locus_tag="PLF85_11915"
     CDS             complement(2141..3739)
                     /locus_tag="PLF85_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE20420.1"
                     /translation="MKKPTKKTLSVKTTVTYNPATGEKVAEYTNTDLRLFPEIVARAR
                     RAQIEWAKLSFRQRAKYVLRMRRYITGHADELALTVSRENGKSRTDALATEVIPCSLA
                     VKWYATNASRVLKEKNLRRSSWLFFNKQSKIIRVPWGVVGIISPWNYPLSIPFGEVVM
                     ALMAGNAVILKVAAVTLSCGKAIEDIVKSAGLPDGLFTHVIGSGGEVSTAMINGGVGK
                     IFFTGSVPTGKKLMAQAAEKLVPLSLELGGKDPMIVLNDADLERATNGAAWAGYQNAG
                     QSCGGVERIYVQSGVYQQFVELLAQKTRAMRHGADADFNVDMGAVTTKDQLNTIQSHL
                     KSAIKKGAKVVAESQPAASAGKLGNFMPATLVTNVDHTMPLMREETFGPILPVMKFET
                     IEEAIQLANDCSMGLTASIWTKSNKLGKKIAPQIQAGVITINDHLYTHGLSETPWGGF
                     KESGLGRTHSALGLEEMTQPQIVNWDLLNPPRNLWWFPFSPETYQALKSALIFSAPLN
                     PLSWLKASLHLAVFSVKKMFSKWE"
     gene            3823..>5358
                     /locus_tag="PLF85_11920"
     CDS             3823..>5358
                     /locus_tag="PLF85_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE20421.1"
                     /translation="MVHLRQLWDRYLRAGDTHFSSIPERKLNRVLNILYALNLTGTLL
                     LNIALVFIFVWLLRHEGERYLGYVPALIVPAGIHVLITFFTIWLKNKTGSFAPTLLGN
                     LSYTGYMVALCLYFGERAGVHLVLLMVMPTTFLVLRYGNWGNLLAHAAIMLSGLVAAL
                     VSYETMQPLFPLPAEISRLGGYLSFLAAIGMLYVYSIHNWRESYAAEQQLIAERDLNH
                     KLLQETIPKLEKAEAKYRHLVDDSDDLIFLAENQLRILSMNRSSQKMLAFLPADMIGK
                     DLCDYIGDGENSDKELSRNLVREQVRNLLATGKQTRFRTRFRHKHREDGVEVLLSLQL
                     SRVNGQDEIIARATEIEPEVTLRFLNREHGSYTLGNNILHADVLSQRIADRISLHYNQ
                     HELNSLRTCIREILINAIEHGNLGISFEEKSRVIEQGDFMAFLRQRQKDPRYANRQVQ
                     VRYIVSRTGLFLRITDEGDGFDHQGFLKRTESDDSLLMLEHGRGLTMARNAFDSVQFN
                     TKGN"
BASE COUNT         1233 a         1491 c         1447 g         1187 t
ORIGIN      
        1 actatttaca gaagaaccca aatggctata cgtgccatcg catttatttt aatagttatc
       61 tataaattcg tagccaaatc agctggcgca gccagctgat tcggcaaata actccatgaa
      121 aaagacattt tatgaactgg gtttatcccc aacgctcgtg caggccgtta ccgacatggg
      181 ctttcgcgag gcttcgccga ttcaggaaaa ggccataccg atactgctcg agggccatga
      241 cctgatcggc caggcgcaga ccggtacggg caaaaccgcc gcgtttgcaa tacccgtcat
      301 cgagaagatt acggcggcaa aagagctgca ggcgatggtg ctctgcccga cgcgtgaact
      361 cgcgatgcag gttgcggccg agttcaagaa gctgctcaaa tacaagaaag aaatccaggt
      421 ggtaaccgtc tacggcgggc aacccattgg cgcacagttc cgcgacctga aagaaaaacc
      481 gcagatcgtt gtgggtaccc ccggccgcgt gatggaccac atgcgccgca agacgattaa
      541 gatgaatacg gtgaagttct tcgtgctcga cgaagccgac gagatgcttg cccagggttt
      601 tcgcgaagat atcgaacaca ttctcaccga tgcgcctgaa gaccgccaga ccgtgctgtt
      661 ttcggcgacg atgtcaaaag aaattttggc gatcacctct cagttcttaa ataacccgca
      721 gcgcgccgac gtcctggcag gcgccagtaa caaaccgaag atcgagcagt ttttcctcat
      781 cgtgcccgaa aaaaaccgcg tcgaggcgac tatacgcctg atggattttt atggcttcaa
      841 gtcagcgctt atcttcagca acatgcgctc gcaggtggac tctctgaccc agcagttcaa
      901 tgaacgcggc atgactgctg aaggtattca cggcgacctc aaccagaacc agcgcgacaa
      961 ggtgatggcg cgcctgcgca gcggccacgc gcggctgctg gtggcaaccg acgtggcggg
     1021 ccggggtatc gacatcagcc acatcgaggc agttttcaac ttcgatctgc cgcgcgacgt
     1081 cgaagactac gtgcaccgca tcggccgtac cggccgcgcg ggtaagagtg gatgcagttt
     1141 ttctttcgtg cgcccgacag aactgggcaa gctccgccgg ctggaaaagc ttaacaacat
     1201 acagattctg ccgcatgacg tgcccgcggc cgaagacctg caggggaaaa aagtttcaac
     1261 gctgctggca gagcttgtcg atatcacgaa acgcgccaag ctggcacagt acctcgagta
     1321 cctgcacgat ctgacgagtg aagaaatctc gcccgaagaa atagccgcgg cagcgatcaa
     1381 gatgctgctc gagaaagacg gcaggcaata cgacaacaag gtgcaattta ctactgccaa
     1441 agatgagctg aaaaaagcac cgggcgataa gcaccggccg cgcaactacc cgccgcgttc
     1501 cggaaagaaa ttttaccgga acaagcggaa caaaaagaac tagctcatca gccgaatggc
     1561 cggcccgaaa taaacgcttt ttcaaatttc cgtttgtgca atcgtgtttt cattgtcacc
     1621 gttcggtcct tgaagcggat cgtgatggtt tgtgtcagta tgtcgggcag cgccagggat
     1681 gcatattcga aatgatagct gtaagttacc acatgcgacc cgcgaaaggg gctctgctgt
     1741 agctccggtg gcagcagcag gctgagtgat tcagtatttg cattcttcct gatttctact
     1801 atactttttc cattccagta agagacgaca agatcacttt tcgttgcggg aatgcgggta
     1861 ccgtcttttt cgtcggccaa gtcgactgca agggattcga gatttgttgc cacgtcctgc
     1921 gattcggaaa aaataaaata ggaccgtatc ttgcctgcat cctccaggtc aaaatacgtt
     1981 gggttcagat cggcattgac cggtttgcca ccgatggtct tgctgtacat ctgtggcttt
     2041 tcgggtacat accgatactc tactgaactg cagcgtgcga aaacaaaggc agccaggaat
     2101 ccgatattgc gcagatacat gccctgcctg ctgctcctga ctattcccac ttactgaaca
     2161 tctttttcac cgagaaaacc gcgaggtgca gtgatgcctt cagccacgac aacgggttga
     2221 gcggcgcaga aaaaatcagc gctgatttaa gcgcctggta ggtttctggc gagaacggaa
     2281 accaccaaag gttgcgcggc gggttcagca ggtcccagtt gacgatctgc ggctgtgtca
     2341 tttcttcaag acccaaagcc gagtgcgtgc ggccaagccc gctttctttg aaacctcccc
     2401 atggagtttc agacaggccg tgcgtataaa ggtggtcgtt gatggtaatg actcccgcct
     2461 gtatttgtgg tgcaatcttc ttaccgagct tgttactttt ggtccagatc gacgccgtga
     2521 gacccatgct gcaatcattg gccagctgta ttgcttcttc aatcgtttca aacttcatca
     2581 cgggcagaat ggggccaaag gtttcttcgc gcatcagcgg catcgtgtgg tcgacatttg
     2641 tcacgagcgt tgccggcata aagttgccga gcttgccagc gcttgcggca ggttgcgact
     2701 ctgcaacaac cttggctccc tttttgatgg cgctcttgag gtgtgactgt atggtattca
     2761 gctggtcttt ggtcgttacc gcacccatgt cgacattgaa gtctgcgtcg gcgccatgac
     2821 gcatggcgcg cgtcttctgt gccaatagtt cgacgaactg ctggtagaca ccgctctgca
     2881 catagatgcg ctcaacgccg ccgcacgact ggcccgcatt ctggtaacca gcccacgcgg
     2941 ctccgttggt agcgcgttca agatccgcat cgttcaacac gatcatcggg tctttgccac
     3001 ccagctcgag cgacaggggc accagcttct cagctgcctg cgccatcagc ttcttgccgg
     3061 tgggtactga gcccgtgaaa aagatcttgc ccacgccgcc gttgatcatt gcagtcgaga
     3121 cttcgccgcc tgagccaatc acgtgagtaa acaggccatc gggcaggcct gcgctcttca
     3181 cgatatcttc aattgccttg ccgcacgaaa gcgtgactgc cgcgaccttc agaatgactg
     3241 cattgcccgc catgagtgcc atgaccactt cgccgaacgg aatagagagc gggtagttcc
     3301 acgggctgat gatgccgacg acgccccagg gtacgcggat gatcttggat tgtttgttga
     3361 agaagagcca gctcgagcgg cgcaggtttt tctctttgag aacgcgcgac gcgttcgtgg
     3421 cataccattt aaccgcgagc gaacacggaa tgacctctgt ggcaagcgcg tcagtacgcg
     3481 acttgccgtt ttcgcgcgag acagtgagtg caagttcgtc cgcgtgcccg gtaatatagc
     3541 ggcgcatgcg caacacgtac ttcgcgcgct gcctgaagga taattttgcc cattcgatct
     3601 gtgcgcggcg cgcccgggcg acaatttcgg gaaacaggcg gaggtcggta ttggtgtact
     3661 cggcaacctt ttcgcccgtg gcggggttat aggtgacggt agttttgacg cttagagtct
     3721 tcttggtagg cttcttcatg gcagaagagg caaacttcgc gcggcgccgt aaagtggttt
     3781 tgtcatactt tacaacctgt accccatggt gaaccacgag ccgtggtaca ccttcggcag
     3841 ctctgggacc gttacctgcg tgctggtgat acgcacttta gcagcatacc cgaacgcaag
     3901 ctcaaccgcg ttctcaacat tctctatgcc ctcaacctga cgggcacgct gcttctcaat
     3961 attgccctcg tcttcatttt tgtctggctg ctgcggcacg aaggcgaacg ctacctcggc
     4021 tatgtacctg cgctgatcgt acccgcaggt attcatgtac tcatcacctt ttttaccatc
     4081 tggctcaaga acaaaaccgg cagctttgcc cccacgctgc tcggaaacct gtcttacacg
     4141 ggctacatgg tggcactctg cctctatttc ggtgaacgag cgggagttca ccttgtgctg
     4201 ttgatggtga tgcccacgac attccttgtc ctgcgttacg gcaattgggg caacctgctc
     4261 gcgcacgcag ccatcatgct gtcaggttta gtcgccgcgc tggtgagtta tgagaccatg
     4321 cagccgctgt ttccattgcc tgcagagatt agccggctcg gcggttacct gtcgtttttg
     4381 gcagccatcg gcatgctcta cgtctactcc atacacaact ggcgcgagag ttatgctgcc
     4441 gagcagcaac tgatcgccga gcgcgatctg aaccacaagc tgctgcaaga aactatcccc
     4501 aaactcgaaa aggccgaagc aaaatatcgc cacctcgtcg atgattcaga cgacttgatt
     4561 tttctcgccg agaatcaact cagaattctg agcatgaacc gctcgtcgca gaagatgctg
     4621 gcattcttgc cggcagacat gatcgggaag gatctctgtg actatatcgg cgacggtgaa
     4681 aacagcgaca aagaactgag ccgcaacctc gtgcgcgagc aggttagaaa cctgctggct
     4741 acaggcaagc agacacggtt tcgtacgcgc tttcgccaca agcaccgcga agacggggtt
     4801 gaggtcctgc tcagcctgca gctgagccgt gtgaacggcc aggacgaaat catcgcacgc
     4861 gcgacggaga tcgagccaga agtaacgctc cgcttcctta accgcgagca tggcagctac
     4921 acgctgggca acaacatact gcatgccgat gtgcttagcc agcgtatagc cgaccgtatc
     4981 tcactgcact ataaccagca cgaactcaat tcgctgcgta cctgcattcg cgagatcctg
     5041 atcaacgcca tcgagcacgg caacctgggt atttcgttcg aagagaagag ccgtgtcatt
     5101 gagcaggggg attttatggc attcctgcgg cagcggcaaa aagacccacg gtatgcgaac
     5161 cgacaggtcc aggtccgtta catcgtgagc cgcacaggcc tgtttttgcg catcaccgac
     5221 gaaggcgatg gctttgacca ccagggtttt ctcaagcgca cggaaagtga cgacagcctg
     5281 ctgatgctcg agcacggcag ggggttaacc atggcgcgca atgcctttga ttcagtccaa
     5341 ttcaacacaa agggtaac
//
LOCUS       DAOQJF010000233         5352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8902_length_5352_cov_3.761374, whole genome shotgun sequence.
ACCESSION   DAOQJF010000233 DAOQJF010000000
VERSION     DAOQJF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8902_length_5352_cov_3.761374"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLF85_11925"
     CDS             complement(<1..552)
                     /locus_tag="PLF85_11925"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE20422.1"
                     /translation="MSRISRSIRMSDRYLRRHFTILNQQDLLGFSIWLGSIAGMIGMA
                     ALYFLGIAPAWLVIVVNAIFASFLHELEHDLIHSLYFKETWVEKMMMWGVWIFRLNTP
                     SPFYRKKIHLLHHKESGQPADIEEQMIGNGMKWDIKRVITMLDQGLAFLINARRVGKT
                     APKLDMKEMARAAFPFTYLYQGTS"
     gene            complement(608..1474)
                     /locus_tag="PLF85_11930"
     CDS             complement(608..1474)
                     /locus_tag="PLF85_11930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE20423.1"
                     /translation="MFNERAMRTAPTKRPEKILVRKPQWTFKELVGQKPRSEFGRLLF
                     GAMSLVFPEGERFFIRSVKQFASQIDDAALGQDIKDFIGQEAQHGKVHETFNKAVLGH
                     DYDIEPFLKWYKSTMFDFVEQKATEILGPKLALSITAAAEHFTATWAAHGLSSGYLEE
                     HITNQNMLDFMQWHAVEEIEHKHVAFDVLQQIDDSYALRAAGMLFCAALLPFVISVAF
                     VSLVRQAKNTDIFAFFRDALSEGANGGIASSFLPAIVEYLKPGFHPSDHDDYEMARRV
                     AQGIEQRMEKVA"
     gene            complement(1640..2068)
                     /locus_tag="PLF85_11935"
     CDS             complement(1640..2068)
                     /locus_tag="PLF85_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20424.1"
                     /translation="MAEKTERLSVHVEAYVDSGAFIAFFDKSDTWHARFAGLFARSKG
                     LATTTLVIAETHGWFLRRFDSYRALEFMAFIESLNILHTIPADADLINNATGILRKYS
                     DQSLTLADACGLYLLKKNPKWKNWSTDRHLELTGRKLVIH"
     gene            complement(2052..2276)
                     /locus_tag="PLF85_11940"
     CDS             complement(2052..2276)
                     /locus_tag="PLF85_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20425.1"
                     /translation="MIRTQISLEKEEMEVAHAAAESFGISLAELMRRALRKEFPPAGG
                     KPWMKYCGSITSGKSVTGDEIDELVYGRKD"
     assembly_gap    2409..2508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2622..3803
                     /locus_tag="PLF85_11945"
     CDS             2622..3803
                     /locus_tag="PLF85_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE20426.1"
                     /translation="MAQYEGLMDFTISDEMQEMLEMAHELCRNEVVPRRAEMDEHEKF
                     PHEIFQKFAQAGLFGIMFEEKYGGLGLSPFLTVLAIEVMSEYCLGVATSAGAIKLGAL
                     PIEHGGTEEQKMKYLTPLAKGEKIAAFALTEPGAGSDVPNLKTFAEKKGDRYILNGTK
                     QWITNAGVADIYTVFALTDKTKGPRGISCFIVEKGTKGLSFGKLEQKMGIRCSHTRQV
                     ILEDVEVPAENLVGLKPNAGFIHAIKTLISSRPFIATMGTGLATGAYKEALKYARQRE
                     QFGKNIINFQAVQHMLAEMVTKIEASRLLAYRAAKYSLNNHPESARYSAMAKLISSET
                     AVQVCNDALQLHGGYGFTKDYPVEKMYRDAKILTIYEGTSQIQKNEIGAYCIKDAGQL
                     K"
     gene            3811..4350
                     /locus_tag="PLF85_11950"
     CDS             3811..4350
                     /locus_tag="PLF85_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20427.1"
                     /translation="MLRKLTSTLAGYLVLYGEVTQEIGRIAAGFFSNRWQRFRALPAS
                     EQAFTLLTAIVIIFTFLPWRAYQIRFGDDPARRHGIYSDDFALILLGCLLAALPLIWY
                     ILPAEPQKLPRSNIFRLGGAILVLGFALLNWIFPQRIAATKEATFAWSFYVFQLVAVL
                     WVITGILGGRTYAQYPVKN"
     gene            complement(4806..>5352)
                     /locus_tag="PLF85_11955"
     CDS             complement(4806..>5352)
                     /locus_tag="PLF85_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNE20428.1"
                     /translation="KDILNLVYICRQNGLAEVASYWQAVIDMNDYQMRRFVSQVIETQ
                     FNSVSGKKIAILGFAFKPDTNDTRESPAIYICKRLIEEKARLFIHDPQALDHAKQDLK
                     GIDSTVTYTESIDEAVEGAHAIVILTQWKQYAELDYARIYGRMVKPAFIFDGRSIVDA
                     QAMHKIGYNITQIGIAPLTYF"
BASE COUNT         1234 a         1410 c         1363 g         1245 t
ORIGIN      
        1 cgatgtaccc tggtagaggt aagtaaacgg aaaagcagca cgagccattt ctttcatgtc
       61 gagcttcggc gcggtcttgc cgactcggcg cgcgttgatc aaaaaggcaa ggccctggtc
      121 gagcatcgtg atcacgcgct ttatgtccca cttcatgccg ttgccgatca tctgttcttc
      181 aatgtctgcc ggctgacccg attctttgtg gtgcagcagg tgtattttct tgcggtagaa
      241 agggctgggc gtgttcaggc gaaaaatcca gacgccccac atcatcattt tctccaccca
      301 tgtctctttg aaatagagcg agtggatcag gtcgtgctcg agctcgtgca gaaacgaagc
      361 gaagatcgca ttcaccacaa tcacgagcca ggccggcgca atgcccagga agtagagcgc
      421 ggccatgccg atcatgccgg cgattgaacc caaccagatc gaaaatccca gcaggtcctg
      481 ctggttgaga atcgtgaagt gcctgcgcag gtagcggtca gacatacgaa tggagcgcga
      541 gatgcggctc actctttcag cggccgttag gctcatggga ggtattcgtg tgggcatgat
      601 agtctcctta tgctactttt tccatacgct gctcgatacc ctgcgcaacg cgccgggcca
      661 tctcgtagtc gtcgtgatca gaaggatgaa aacctggctt caggtattcg acaatcgcgg
      721 gcaggaaaga cgacgcgata ccgccatttg ctccctcaga gagcgcatcg cggaagaatg
      781 cgaagatgtc ggtattttta gcttgtctca ccagtgagac aaaagccacg ctaatcacaa
      841 acggtagcag cgcggcgcag aacagcatgc ccgcagcgcg cagcgcgtaa gagtcatcga
      901 tctgctgcaa tacatcaaac gcgacgtgct tgtgttcgat ttcttcgaca gcatgccact
      961 gcataaagtc gagcatgttc tggttcgtga tatgttcttc gaggtaacca gagcttaagc
     1021 catgggcggc ccaggttgcg gtgaagtgtt cggccgcggc agtaatcgac agcgcgagct
     1081 tgggtccgag aatttctgtc gccttctgct cgacaaaatc aaacattgtg gatttatacc
     1141 atttcagaaa cggctcaatg tcgtagtcat ggcccagcac tgctttgttg aaggtttcat
     1201 gtaccttacc gtgctgcgcc tcttgcccga taaaatcttt tatatcctga ccgagtgctg
     1261 cgtcgtcgat ctgcgaagca aattgtttca cgctgcgaat aaagaagcgt tcaccttcgg
     1321 gaaacaccag gctcattgca ccgaaaagca ggcgaccaaa ctcagagcgg ggtttctgcc
     1381 ctacgagttc tttaaaggtc cactgtggtt tacgcaccag gattttttcc gggcgttttg
     1441 tcggcgccgt acgcatcgct ctttcgttaa acatgtttcc tccacaggat ttgtttcagt
     1501 aggacgaacg gtccaaataa ctgaacaatc cgtcaacttt gaaaagatag ataaagttga
     1561 acaaagcgtc aaatattttt cggttttcat cgccctttgt tcatatgggt agattgaaac
     1621 ctacccatat gaacagaacc taatggatca ctaatttacg gccggtaagc tcgaggtggc
     1681 ggtcggttga ccagttcttc cactttggat ttttcttgag cagataaagc ccacaggcat
     1741 cggcaagtgt caggctttgg tcagaatatt tgcgcagaat gccggtagca ttatttatta
     1801 aatccgcgtc agcaggaata gtgtgcagga tattgagaga ctcaatgaac gccatgaatt
     1861 cgagcgcccg gtagctgtcg aagcgacgca gaaaccaacc atgcgtttcg gcaattacga
     1921 gtgtggttgt cgcaaggcct ttgctgcggg caaagaggcc cgcgaaccgg gcatgccagg
     1981 tatcactttt atcgaaaaaa gcaatgaacg caccgctgtc gacatacgct tcaacgtgta
     2041 ctgacagccg ttcagtcttt tcggccatag accaactcgt caatttcatc gccggtcacc
     2101 gatttgcccg aagtgatgct accgcagtat ttcatccagg gtttgccgcc ggccggcgga
     2161 aattctttgc gcagcgcacg tcgcattagc tcggccagcg aaatgccgaa gctctcggcc
     2221 gcagcatgag ccacctccat ttcttccttt tcgaggctta tctgtgtgcg tatcatgata
     2281 acaaaaatat acatcatgtt atcatacgtc aatcaaaaaa acctcgtgtc gccttctgat
     2341 ggtgaacgtt gtgtcaaatc agcttgtccg actgtttaaa aattttgctc tgtcatagac
     2401 ttcgcacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc aaatcagctt
     2521 gtccgactgt ttaaaaattt tgctctgtca tagacttcgc acaagagaca aggcgctgca
     2581 ggcgccttgt ctcttgtgcg gttaccccaa aggagaaaca aatggctcaa tacgaaggct
     2641 tgatggattt tacgattagc gacgaaatgc aggagatgct cgaaatggcg cacgaactgt
     2701 gccgtaacga agttgttccc cgccgcgctg aaatggatga acacgagaag tttccacatg
     2761 agatcttcca gaaatttgca caggcaggcc tctttggcat catgttcgaa gaaaagtacg
     2821 gtggcctggg cctttctcct ttccttacag ttctcgccat cgaagtaatg agcgaatact
     2881 gcctcggcgt tgctaccagc gcgggcgcaa tcaagctcgg tgctctgccc atcgaacacg
     2941 gcggtaccga agaacagaaa atgaaatacc tcaccccgct ggcaaagggc gaaaaaatcg
     3001 ccgctttcgc actgaccgag ccgggtgctg gttcagacgt gcctaacctc aagacttttg
     3061 cagagaagaa aggcgatcgt tacatcctga acggtaccaa gcagtggatc accaacgcag
     3121 gtgtcgctga tatctacact gtgtttgccc tgaccgacaa gaccaagggc ccacgcggta
     3181 tctcttgctt tatcgtcgaa aaaggcacca agggcctgtc attcggcaag ctcgagcaaa
     3241 aaatgggtat ccgctgctcg cacacacgcc aggttattct tgaagacgtt gaagtgccgg
     3301 cagaaaacct cgtgggtctg aagcccaacg cgggcttcat acacgcgatc aagacgctga
     3361 tctcctcacg tccgtttatc gcgaccatgg gtaccggcct cgcaacaggc gcgtacaaag
     3421 aagcactcaa gtacgcacgc cagcgcgagc agttcggcaa gaacatcatc aacttccagg
     3481 cagtgcagca catgctcgct gaaatggtaa ccaagatcga agcttcgcgc ctgctcgctt
     3541 accgcgcggc gaagtactcg ctcaacaacc accctgagtc agcgcgctac tccgcgatgg
     3601 caaagctgat ttcttcggaa acagcggtgc aggtctgcaa cgacgcactg cagctgcacg
     3661 gcggctacgg cttcaccaaa gactacccgg tcgagaaaat gtaccgcgat gcgaagatcc
     3721 tcacgatcta cgaaggtacc agccagatcc agaaaaacga gatcggcgcg tactgcatca
     3781 aggacgctgg ccagctgaaa taaggtgggg ttgctccgca aactcacttc gacgcttgcc
     3841 ggttacctcg tgctctacgg cgaggtaacg caggaaatcg gccgcatcgc ggccggtttt
     3901 ttttctaacc gctggcaacg ctttcgcgcg ctgccggcca gtgagcaggc attcaccctg
     3961 ctcactgcta tcgtcattat cttcaccttt ttgccctggc gtgcctacca gattcgtttc
     4021 ggcgacgacc cggcacgccg ccacggtatt tatagcgacg acttcgcact cattttgctc
     4081 ggctgcctgc tggcggccct gcccctcatc tggtatatct tgcctgcaga accgcagaag
     4141 ctcccccgct ccaatatttt tcgtttaggg ggagctatac ttgttctggg ttttgccctc
     4201 ttgaactgga tttttccgca gcgcattgcc gcgacaaaag aagccacctt tgcgtggagc
     4261 ttttacgtgt ttcagctggt ggcagtgttg tgggtaataa cagggatact gggcgggcga
     4321 acctacgccc agtatcctgt taaaaactga gtggtctcgg cctcggccgg acttgcagct
     4381 ttcgctacct gccctgactg gaccccgcca cccagctctc tttcgagaat ttcacgaaag
     4441 ttcggccctt gcggtaccgc aacggtcagt accggctggc gcttgaccag ctgatagtaa
     4501 ccaggctttc gctggtattc gcgggcgccc ctctgcgagc tgcctgcaat tgccggagca
     4561 atcgaatggg tgtttactgt tgtggttttc atagtctccg cagagacacc cattcgaaaa
     4621 ccatttgaat atcgacgcct gtgagaaaaa cctgaagctc ttcttaccag tctatgcgac
     4681 ataatatact tagacgttat gggcggcaag aaaaaaacga aaaaatgtag gtgctcgtct
     4741 cgactgcgct cgacgaccga atatgcccgg tcgctgagcg cagccgaagt gaccgagtga
     4801 gcaaatcaga agtaagttaa cggcgcaatg cctatctggg tgatgttgta cccgatcttg
     4861 tgcatggcct gtgcgtcgac aattgaccgg ccatcgaaga taaaggcagg cttcaccatg
     4921 cggccgtata tgcgagcata atcgagctcc gcatactgtt tccactgcgt cagtatgaca
     4981 atcgcatgag ctccctcaac cgcttcgtcg atgctctcgg tgtaggtgac ggtactatcg
     5041 atacctttga gatcctgttt cgcgtggtcg agcgcctgcg ggtcgtgaat gaatagccgc
     5101 gccttttctt cgatgaggcg cttgcagatg tagatcgcgg gtgactcacg cgtgtcgttg
     5161 gtgtcgggtt tgaaggcgaa cccgagaatc gcaatttttt tgcccgagac gctgttgaac
     5221 tgcgtttcaa tgacctgcga gacgaagcgc cgcatctggt agtcgttcat gtcgatgacc
     5281 gcctgccagt aactggcaac ttcagcgagg ccgttctgcc ggcagatgta aaccaggttt
     5341 agaatatctt tc
//
LOCUS       DAOQJF010000234         5350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8907_length_5350_cov_3.513126, whole genome shotgun sequence.
ACCESSION   DAOQJF010000234 DAOQJF010000000
VERSION     DAOQJF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8907_length_5350_cov_3.513126"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..851
                     /locus_tag="PLF85_11960"
     CDS             237..851
                     /locus_tag="PLF85_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNE20429.1"
                     /translation="MEKVRDYYADYLQHLINGDRSACEQIIRACLGQNLPYAEIYSDL
                     IQHSLYEVGELWANDKITVAQEHLASSITEFLLVIIHQRLFQKRPSPRKAIIACTQNE
                     MHQIGANRLEIRGWDSSFLGSNTPSDGVLATIDKIKPQLVGLSVTLTRNLPKVAEVAQ
                     QIRQQFPAVRLLIGGQAAYTREAQNFAAIHDYVRPIGAIETELV"
     gene            856..1932
                     /locus_tag="PLF85_11965"
     CDS             856..1932
                     /locus_tag="PLF85_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE20430.1"
                     /translation="MPLTAEALFHSENIPIIEIDAQHRVLQIGKALVMRMPRLAPGDD
                     VRLVFPNLPLAQLLGNSTLRARINLYKEDTSPVSHDVFAVKHEEKVFLVFEPVIDDIQ
                     RLQQEIIRMNNDLSNAMREIQKQNAELSQLNNLKNEFLGMAAHDLRTPIANIYSFSDL
                     ALLDTEKLDAELLQYLTEIKALAEFMMELLSDLLDITVIESGNLHLKREPVSLAVILD
                     RAIRLHALMAVKKGVRIIAQLETDERAVNVDSLKVAQVCNNLINNAIKFSSSNSEILV
                     TAGYGDSECWFEVIDHGPGIEASQVAHIFDPFRKGSLQPAGNKGHGLGLAISKKIVDA
                     HGGRLQVQSIPGQGATFRVTLPAE"
     gene            2029..4200
                     /locus_tag="PLF85_11970"
     CDS             2029..4200
                     /locus_tag="PLF85_11970"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE20431.1"
                     /translation="MAFLSAEFRRSPFFQLSWRLETFTSVIPIPTFVYFIMVTGAFEG
                     EARIWAVGIAGLIAGGGTVAWGLIYRWFALKKLMAELTRLKTLDDAPLADKIKLRHRL
                     LAYPYREGRTIIARWSVGVISGALIYIVQMQEVPFYALFVEAYSLLFVLPISYVMYLF
                     MSESVLRPVLQDPLFAGLPAAGRRIRETSFFTRLLITIASVTITPIAIFGFMLYTTVS
                     GRLSFKHPEVHIVLLSLEALAAIVIVAYRTAKSVRLSIRDTNDVLQQLGKGDFAVNSA
                     RATTDEFGRQAELVGAVAHDLSRMYREVNELNETLEQKVEQRTAELNQTLEQVRHLKR
                     HQDGDYFLTTLLLKPLNSNYVKSDRVGVEFFLRQKKDFEFKGVSHDLGGDLCSAHMVR
                     LRDSEYVVFLNADAMGKSMQGAGGALVLGAVFYSIVERTRSVPSMQQLAPERWLKNAF
                     LELDKVFESFDGSMLVSLVLGLVDTQTGLLYYVNAEHPWSILYRGNSASFIENELSLR
                     KLGMSADGRITVRVFEMQPGDVLICGSDGRDDILLHTPDGRPKMNEDEDLILSAVVSA
                     KGELPLVYETCAQRGEITDDYSLLKISYFGDAMPETEKKAMRSASGAYYRAQNYLKAI
                     AEGEKYLSSRPADTQFMRFLSRCHRLAGNYEIAIDLAERLRLRQPDDTRNLTNLAAMH
                     ILRGEPARAEQLIAEIRALDAQYPALRKLDELMHRRSRRGR"
     gene            4197..4403
                     /locus_tag="PLF85_11975"
     CDS             4197..4403
                     /locus_tag="PLF85_11975"
                     /inference="COORDINATES: protein motif:HMM:NF021042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02450 family Trp-rich protein"
                     /protein_id="HNE20432.1"
                     /translation="MSSLSFAKALGSKWTAVHPQNGEKHFIVTRIADHKLRTVKLECA
                     VTRSTRIVAFAELAKGDEFLPGWH"
     gene            complement(4416..5135)
                     /locus_tag="PLF85_11980"
     CDS             complement(4416..5135)
                     /locus_tag="PLF85_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HNE20433.1"
                     /translation="MLSPFWGEFFGTLVLIVLGSGVVAGVLLEKSKAFGSGWIVITAG
                     WAFAVTLGVFTAKTFGSADAHLNPAVTLAFAIKSGSFENVPGYIAAQLSGAFTGAIIT
                     WLHYLPHWSETENAELKLGVFATSPAIRHPASNMISETLGTLILVIGIYAIFSRGTGG
                     ITAGMGPFLVGILVWAIGLSMGGTTGYAINPARDLGPRLAHAILPIAGKGSSDWKYAC
                     VPVVAPLIGGALGGMLMRLAG"
BASE COUNT         1172 a         1489 c         1521 g         1168 t
ORIGIN      
        1 cgatcagcaa catcaggaaa aattttggca gtgcctgccg aagctgtacg tggtacatga
       61 ggcactgcgt tgagcaggcg acattgcggc aagtgccaat ttcggttacc ggtggtgggt
      121 ctggttagcg gtgttcgggg gcagaattac ccataaacta attgcgaccg cgacagaaat
      181 acccgaactg gccttcaggc attcgtatac cccacagggt gctgcttttt gggcctatgg
      241 aaaaggttag agactattat gcggattacc ttcagcacct gattaacggc gacaggtcgg
      301 cctgcgagca aattatccgg gcctgtctcg ggcagaacct gccttatgcg gaaatttatt
      361 ctgatctgat acagcactct ctctacgagg ttggtgaact ctgggccaat gacaagatta
      421 cggtcgcgca ggagcacctt gcgtcgagta tcacagagtt tttgctcgtg atcattcacc
      481 agcggttgtt tcaaaaacgg ccctcaccgc gcaaggcaat cattgcctgt acgcagaatg
      541 aaatgcacca gatcggcgcc aaccggctcg aaattcgagg gtgggatagt tcctttcttg
      601 ggtctaatac gccctcagac ggggtgctgg cgaccatcga caagatcaag ccgcagctcg
      661 tgggtctttc ggttaccctt accaggaacc tgcccaaagt tgccgaggtt gcgcaacaga
      721 tcaggcagca gtttcccgcg gtaaggttac ttatcggggg ccaggcagcg tacacgcgtg
      781 aggcacagaa cttcgctgca attcacgatt acgtacgccc gattggcgcg atcgaaacgg
      841 agttagtgta agaccatgcc gctgacagcc gaagctcttt tccattctga gaatattccg
      901 attattgaaa tcgatgcgca acacagggtg ctgcaaatcg gcaaggctct ggtgatgcgt
      961 atgccccgtc tggcgccggg tgacgatgtc cggctcgtat ttcctaatct gccgcttgcc
     1021 cagttgctgg gcaattccac tctgcgtgcg cgcatcaacc tctacaaaga agacacgagc
     1081 ccggtcagtc acgatgtctt tgcagtgaag cacgaagaaa aagtctttct ggtcttcgaa
     1141 cccgtgatcg atgacatcca gcgcctgcag caagagatca tccgcatgaa caacgatctc
     1201 agcaacgcga tgcgcgagat ccagaaacag aacgccgaac tctcgcagct gaataaccta
     1261 aaaaacgagt ttctcgggat ggctgcgcac gacctcagaa cgccgatcgc caatatttac
     1321 agctttagcg atcttgcgct gctcgatacc gaaaaactcg atgcagaatt gctgcagtac
     1381 ctgaccgaaa tcaaagcgct ggctgaattc atgatggagc tgctcagtga tttgctcgac
     1441 attacggtga tcgaatcagg caacctgcac ctaaaacgcg aacctgtgtc gcttgcggtc
     1501 attcttgacc gtgccatcag gctgcatgcc ctgatggcgg ttaaaaaggg tgttagaatt
     1561 atcgcacagc ttgagaccga tgaacgggca gtgaatgttg acagcctgaa agtggcgcag
     1621 gtctgtaata acctgattaa caacgcgatc aaattttcat ccagcaattc agagattctg
     1681 gtcactgcgg gctatggtga cagtgaatgc tggttcgagg taatagacca cggtcctggt
     1741 atcgaagcaa gccaggtggc acatatcttc gacccgtttc gcaaaggcag tcttcagccc
     1801 gcaggcaaca aaggccatgg tctcggtctt gccatcagca aaaagattgt cgatgcgcat
     1861 ggtggccggc tacaggtaca gagcattccg gggcagggtg cgacgtttcg cgtcacgctg
     1921 cctgcagaat aaacgttaac ctgccgatag gcagtttatc ttaggaccct gctattcgga
     1981 aaaacctttc cgatttaccg ctgcagcatc tcccgtaccg tggtggatat ggctttcctt
     2041 tcggcggagt tcagacgttc gccctttttt cagctgagct ggcggctcga gaccttcaca
     2101 agcgttatcc cgattccgac atttgtctac tttataatgg ttacgggtgc gttcgagggt
     2161 gaggctcgca tctgggctgt gggtattgcg ggactgattg ccggtggtgg caccgtcgcc
     2221 tggggcctca tctaccgctg gttcgcgctg aagaagctca tggcagaact cacccggctg
     2281 aagacgctcg atgacgcacc actggccgac aagatcaagc tgcgccatcg cctgctcgca
     2341 tacccctacc gtgagggccg caccatcatt gcgcgctggt ctgtgggagt catcagcggt
     2401 gccctgattt acatcgtgca gatgcaggag gttcccttct acgcgctttt tgtcgaagcg
     2461 tattcactgc tcttcgttct gcccatctcg tacgtcatgt acctgttcat gtccgagagt
     2521 gtgttgcgcc cggtactgca ggatccgctc tttgcgggtc tgcctgcagc gggtcggcgt
     2581 attcgcgaga cgagtttctt tacgcgactt ttgatcacga ttgcttcggt caccatcacc
     2641 ccgatcgcga ttttcggttt catgctctac accacggtct cgggtcggct atcgtttaag
     2701 catcccgaag tgcacattgt gctcttgtcg ctcgaggcac tcgcggcgat tgtgattgtt
     2761 gcgtaccgca cggcaaagtc ggtcaggctc agcattcgcg acaccaatga cgtgctgcaa
     2821 cagctgggta agggtgactt cgccgtgaac agcgcgcgcg ccactaccga tgaattcggg
     2881 cggcaggcag agctcgtcgg cgcggttgca cacgacctga gccgcatgta ccgcgaagtg
     2941 aatgagctca acgagactct cgagcagaaa gtcgagcagc gcacggcaga gctcaaccag
     3001 acactcgagc aggtgcgcca cctcaagcgc caccaggacg gtgattattt tctcacgacg
     3061 ctgcttttga aacccctgaa ctccaactac gtgaagtcag accgcgtggg ggtggagttt
     3121 ttcctgaggc agaaaaagga tttcgaattt aagggtgtct cgcatgacct gggcggcgac
     3181 ctctgcagcg cgcacatggt gcgcctgcgc gacagcgagt acgtggtatt tctcaatgcc
     3241 gacgcgatgg gcaagtcgat gcagggcgcc ggtggcgcgc tggtgctggg tgccgtgttt
     3301 tattcgatcg tcgagcgcac gcgctctgtg ccgtcgatgc agcaactggc gcccgaacgc
     3361 tggctcaaga acgcttttct cgaactcgat aaggtcttcg agagcttcga cggttcgatg
     3421 ctggtttctc tggtgcttgg gttagtcgat acgcagacgg gccttctcta ttatgtgaat
     3481 gccgagcacc catggtcgat tctctaccgc ggcaattctg ccagcttcat cgagaatgaa
     3541 ctctcgctac gtaagctcgg catgtcggcc gacggacgca tcaccgtacg cgtgttcgaa
     3601 atgcaacccg gtgatgtgct catttgcggt tcggacggtc gtgacgacat actgctgcat
     3661 acccccgacg gtcgcccgaa aatgaatgaa gacgaagacc tgattctttc agccgtagtt
     3721 tcagcaaagg gggagctccc actggtttat gaaacctgtg cgcagcgcgg tgaaattacc
     3781 gatgactatt cgctgctgaa aatttcgtac ttcggcgacg ctatgcccga aaccgaaaag
     3841 aaggcaatgc gttctgcatc gggtgcctac taccgggctc agaattatct gaaagcaatc
     3901 gccgagggcg agaagtacct cagcagcagg ccggccgata cacagttcat gcgcttcttg
     3961 tcgcgctgcc accggcttgc gggcaactac gaaatcgcga ttgatctcgc cgagcgcctg
     4021 cgcctgcggc aacccgacga cacgcgcaac ctgacaaacc tcgcggcgat gcatatactg
     4081 cggggtgaac ctgcgcgtgc agagcagctc attgccgaga tcagggcgct cgatgcgcag
     4141 taccctgcgc tgcgcaaact cgatgaattg atgcaccgtc gctcccgccg cggcagatga
     4201 gttcgctgag ctttgccaaa gcgctgggtt caaaatggac agcagttcac ccgcagaacg
     4261 gggaaaaaca ctttatcgtc acgcgcatcg ccgaccacaa gctgcgcacc gtgaagctcg
     4321 aatgcgcagt gacccgctct acgcgcatcg tggcgtttgc cgagctggcg aaaggcgacg
     4381 agttcctgcc aggctggcac tagcaaggtg ttggttcaac cagcgagtct catcagcata
     4441 ccgcccagag ctccgccgat aaggggagcg acgactggca cgcacgcata cttccagtca
     4501 gagctgcctt tgccggcgat gggtaagatc gcgtgcgcga ggcgtgggcc caggtcgcgt
     4561 gccggattaa tggcgtaacc ggttgtgcct cccatcgaaa ggccaatggc ccagacgaga
     4621 atgccgacga gaaacgggcc catgcccgcc gtgataccgc ccgtaccgcg cgagaagatg
     4681 gcgtagatgc cgatcaccag aatcagtgtg ccgagcgttt cgctgatcat gttcgaggcc
     4741 gggtgcctga tcgcagggct tgtggcaaac acgccgagct tgagttctgc attttcggtt
     4801 tcagaccagt gcggcaggta gtgcagccag gtgatgatgg cacccgtgaa tgctcctgac
     4861 agctgtgctg caatgtagcc gggtacgttt tcgaacgacc cggacttaat ggcaaatgcc
     4921 agcgtcaccg ccgggttcag gtgcgcgtcg gcactgccga aagttttagc ggtgaagacg
     4981 cccagcgtga cggcaaatgc ccagcccgcg gtgatgacaa tccagcctga accgaacgcc
     5041 ttggactttt cgagcagcac gcctgccacg acgccgctgc ccaaaacaat cagaaccaat
     5101 gtgccgaaga attcgcccca gaatggtgaa agcataaacg gtgaaggcag tgcctgcctg
     5161 tcacgtcaag gcgattcggt ctacttaccc cagcttcagc aactggccga gtacggtcgt
     5221 ataaccttcg ggcatcgagc gcgccatgac gtcgatcgct acagcgtcgg cgccaatcag
     5281 aacgcgcggt gatttttttt caatgccctt aacgatcgtt gcagcagctt catcgggtgt
     5341 ggtgcgtgcg
//
LOCUS       DAOQJF010000235         5313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_8993_length_5313_cov_4.739064, whole genome shotgun sequence.
ACCESSION   DAOQJF010000235 DAOQJF010000000
VERSION     DAOQJF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8993_length_5313_cov_4.739064"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="PLF85_11985"
     CDS             complement(<1..2079)
                     /locus_tag="PLF85_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE20434.1"
                     /translation="MKHLSLYFLLLAVAVSGCSKVNTSEEDKDARLFELYVNYPGIKP
                     AGHKPPEFDIRLANLIDNARKEVYWAMYGFYRASIKDSVLRAVQRGLDVQLAGDVGTY
                     SYGEIGYIQFEDLLRRYPNAKLLSGNAQSIQHNKFCVIDRRYIMTGTGNITDSEIDRN
                     YNVWMIIESKEMAEDFINDHQQMMAGRFGHAKSRLDYNNVFNVGQVRVEAYFSPQEDA
                     MSRFLQAVSEAQQSINFAIFAFTHDQLGRLFIQKHKQFTQQNNQDGGSRAVRGIMDRS
                     QLTHNQYVEVYRLATSCGHTYGFEQGAGNANGGNANPFFPNGTNTWDTPTQSNTRCYS
                     PFDLKIDGNENNNYIGDWQAGGGRLHAKTIVIDAGTPNAKLLLGSFNWSPNANNNNDE
                     NLIVIHSPAIVERYMQFWKGIYDDAVPMNKRFPTNQEMQQGIVTYQPREAGDYQKIVI
                     SEVNYAGTSRKIGSNYFAYDGNEFIELYNPTDRAIDVSFWSLYFPVIDSYTDPGVYTS
                     GWPGYESLAKRGLVGLPGGTFIPAKGFLVVTQSDQRNDLNSDGYFFPASVLYDPANTA
                     SYNNKITYYNPVNGLSNFFTLFDRRTSSTGRANVTTSSFTDPFSGKFYPSNGSILTFV
                     YPSFDEGGRCRTAWDAVVDRFSGSNYFNPYHMHAGNESTYTHLTSLWVELRDNRGNLV
                     DIVGGNRTGAA"
     gene            complement(2076..3572)
                     /locus_tag="PLF85_11990"
     CDS             complement(2076..3572)
                     /locus_tag="PLF85_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20435.1"
                     /translation="MKRKRLHILPLAVLVCVSFMRGTCATTTNDPELSTQSMNYYIGL
                     GGNAKAKEKLLSIIGSAKTEVVGVFNDLADTDISSALIEKANAGLKVAVGGDRRNENN
                     AGFAALKSLRSSNKFLDYATATSSAAAEPNQNLKNKILATRLNFNRLNRSLKYNAEPF
                     DGRVEYNFVVADKYNCWVSTGGANSETFSTGFSIVFVFQSFDICNDFYNEAQQLVYGG
                     LFGDEGVPSFGKFRNSKTITDPNTRFRLGDLIFNIYFAPQERPLVALVTELLRAEKNI
                     KFASRAITQDIVADMTNHNSNRSHVLNALQYKARIPQVFGQPFSIKGVMGTEVDGAPT
                     AIPSPWTSYNAPYNALVSGACPTVNTTTLNIPFKDTNGTAVFNNAKINTTSIHCDLKA
                     LQDAIGSTSTAEFRKHPSSLPFSVFMTDVGARKPRLIVMSSDLRKRYYYDDGQSQDAE
                     PKRTQDDLYSITDAFVIMVEPAGTEANMKIFNDFSTLVDRLFNAGGAL"
     gene            complement(3569..4318)
                     /locus_tag="PLF85_11995"
     CDS             complement(3569..4318)
                     /locus_tag="PLF85_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20436.1"
                     /translation="MLAATLLLCANCTTSVGELWPTAINVDRKRGCAEIREVNWAGSM
                     KNDGTYDADDDFIEIQNTDCNKPIDIADWRIELTGDVKRIYYVPKVGNTTVQPGKFAV
                     IIAKAGGAFRDQGNADYTPVVLEGLSIPERNWSITTRTAENFLMESGINTTEADDAQS
                     RNFPLSGSVDGFTTRSMERTEDQFEEEGGSVSTWHASTPCNETSPSQITTLYGTGCGA
                     GQIGVSGRYVHPDYSQRTFATPGEKNTPDYK"
     gene            complement(4423..>5313)
                     /locus_tag="PLF85_12000"
     CDS             complement(4423..>5313)
                     /locus_tag="PLF85_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20437.1"
                     /translation="PRVYAYYARVRGNRGGSDYSENGRIGNFSDNDWSGLYGARVSAH
                     LHDQFTFFKQLIVYGDAALSTGSDLRRQGQPIADYASVGFGAGVTAQTKSLLGFLAPL
                     AEFDFFYSQGAKYDKNGNIISHGFISMRGDRMGGTLMRRYWGMRPSGYVNYGGIDDTP
                     FDANRKAGSLLLHAGIGAEMWKSFVVRADIWHVQDTQSTAVNFDPNNLDLINNPYVSR
                     AELEAQKRFGKALGQEINLTVQYFPNSLLMFQLTGAMFVPGSFFSTPIEDIVSKNGVP
                     KGGPADADFWGIFLRSTLSF"
BASE COUNT         1217 a         1391 c         1386 g         1319 t
ORIGIN      
        1 tgcagctcct gtgcgattgc caccgacaat atcgaccaaa ttaccgcggt tatcccgcag
       61 ctcaacccat agagatgtca aatgtgtgta ggttgattca ttgccagcat gcatgtgata
      121 aggattaaaa tagttcgatc cgctgaaacg gtcgacaact gcatcccatg ccgtgcggca
      181 acggcctccc tcatcaaatg aagggtacac gaacgtgagt atgctgccat tgctcggata
      241 aaactttccg ctgaatgggt ctgtgaaaga cgacgtcgtc acgttcgccc tgcccgtact
      301 gctggtgcgc cgatcgaaca gggtaaagaa gttcgagagg ccgttgaccg gattgtaata
      361 cgtaatctta ttattgtagg aagcagtatt agccggatca tagagcactg aagccgggaa
      421 gaagtacccg tctgagttga ggtcattgcg ctggtcagat tgcgtcacca ccaggaagcc
      481 ttttgcaggt ataaacgtac caccaggcag acctacaagc ccgcgtttag cgagtgattc
      541 gtaacctggc cagccactcg tgtatacgcc ggggtcagta taactatcga tcacaggaaa
      601 atagagtgac caaaaagaga catcgattgc ccggtctgtc gggttgtaga gttcgatgaa
      661 ctcgttgcca tcgtaggcaa aatagttact accgatcttg cggctggttc cggcgtagtt
      721 cacctcagag atgacaattt tctggtaatc gccggcctca cgtggctggt aggtcacgat
      781 accctgctgc atctcctgat tggtcggaaa tctcttattc atcggcactg cgtcgtcata
      841 gatacctttc cagaattgca tgtagcgttc gacgatcgcc ggagagtgaa tcacgataag
      901 gttttcgtcg ttgttgttat tagcattcgg cgaccagttg aacgagccca gcagcagctt
      961 tgcgttggga gtaccagcat caatcacgat ggtttttgcg tgaaggcgtc cgccaccggc
     1021 ctgccaatct ccgatgtagt tgttgttctc gttgccgtcg atcttcaagt caaacggcga
     1081 ataacagcgc gtatttgact gtgtcggcgt gtcccaggtg tttgtgccat tcgggaaaaa
     1141 cggatttgca tttcccccgt tggcgttgcc cgccccctgc tcgaagccgt acgtatgccc
     1201 gcacgatgtg gcaaggcggt agacctcgac atactggtta tgcgtcagct gcgagcggtc
     1261 cataatgcca cggacagccc tcgaaccacc atcctggtta ttctgctggg tgaactgctt
     1321 gtgcttctgt atgaaaaggc gaccgagttg gtcatgagta aatgcaaaaa tcgcaaaatt
     1381 gatactctgc tgagcttccg aaactgcctg caaaaacctg ctcattgcat cttcttgcgg
     1441 tgaaaaataa gcctcaacac gcacctgccc gacgttaaag acgttattgt agtcaaggcg
     1501 cgattttgca tgcccaaagc ggccggccat catctgctgg tggtcattga tgaagtcctc
     1561 tgccatttct tttgattcaa taatcatcca cacgttatag ttgcggtcga tctctgaatc
     1621 agtgatattt cctgtgcccg tcatgatata acggcgatcg ataacgcaga acttgttgtg
     1681 ctgaatcgac tgtgcatttc cggagagcag cttggcattc gggtaacggc gcaacaggtc
     1741 ttcgaattgt atatagccga tttcgccgta tgagtacgtc ccgacgtcac cggcaagctg
     1801 aacatccagg cctctctgca ccgcacgcaa aacggagtct ttgattgagg cacgataaaa
     1861 accgtacatc gcccaataaa cttctttgcg cgcgttatcg ataaggttgg caagacggat
     1921 atcaaattcg ggcggcttat gacctgctgg tttgatgccc gggtagttca cgtaaagctc
     1981 gaacaaccgc gcatctttgt cctcttcaga ggtgtttact ttactacagc cgctgaccgc
     2041 cacagccaga agaagaaaat aaagactgag gtgtttcata acgcgcctcc tgcgttgaag
     2101 agtcggtcta ccagagtgct gaaatcattg aatattttca tattcgcctc tgtacctgct
     2161 ggctctacca tgatcacaaa ggcatccgtg atgctgtaca ggtcgtcctg ggtacgtttg
     2221 ggttctgcat cctggctttg gccatcatcg tagtagtagc gtttccgcag atcggacgac
     2281 atgacgatca ggcgcggctt gcgcgcacca acgtctgtca tgaatacgct aaacggcagg
     2341 cttgacgggt gcttgcgaaa ctctgcggtt gaagtgctgc caatcgcgtc ttgcaatgct
     2401 tttaaatcac aatggataga ggtcgtattt atcttggcat tgttgaacac cgctgtcccg
     2461 tttgtgtctt taaaaggtat gttgagcgtt gtcgtattga ccgtagggca cgcccctgaa
     2521 accagagcgt tatatggagc attataagag gtccagggcg aaggtatagc cgtaggcgcc
     2581 ccgtcaactt ccgtgcccat tactccttta atggagaaag gttgaccgaa gacctgcgga
     2641 atgcgcgctt tgtactgcag cgcgttcagt acatgactgc ggttgctgtt atggtttgtc
     2701 atgtcggcaa cgatatcttg cgttattgct cgcgaggcga atttgatgtt tttttctgcg
     2761 cgcaacagct cagtaacaag cgcaaccagt gggcgctcct gaggggcaaa ataaatattg
     2821 aatatcagat cgcctaagcg aaagcgtgtg ttggggtcgg taatagtctt gctgttgcga
     2881 aacttgccga acgacggaac tccctcgtca ccaaagagac caccgtagac cagctgttgc
     2941 gcttcattat agaagtcgtt acagatatca aatgactgaa aaacgaacac aatggaaaaa
     3001 cccgtcgaga aagtttcgct gtttgcaccg ccggttgaga cccagcagtt gtatttgtct
     3061 gcgactacga agttatattc tacccggccg tcaaagggtt cagcattata ctttaggcta
     3121 cgattgagac ggttaaaatt gagcctcgtt gcgagaatct tattcttgag attttggttc
     3181 ggttcagccg cggcggatga ggtcgccgtt gcataatcca ggaatttgtt tgaggagcgc
     3241 aggctcttga gtgcagcaaa ccccgcatta ttctcattcc gcctgtcacc gcctactgca
     3301 accttaaggc ctgcattagc cttttctatc aatgcgctgc tgatgtccgt gtcagccaga
     3361 tcattgaata caccgacaac ctcggtcttt gctgaaccaa taatgctcag cagcttctct
     3421 ttggccttgg cgttgccccc gagtccaata tagtagttca tcgactgggt gctcagttcg
     3481 gggtcattag tcgtcgtcgc acaggtgccg cgcataaaag acacgcaaac gagcaccgcc
     3541 agtggcaaaa tatgtaggcg ctttcttttc atttgtaatc cggtgtgttt ttttcgcctg
     3601 gcgtcgcgaa agtgcgctgc gaatagtcgg ggtgcacata ccgcccgctg acgccaatct
     3661 ggcctgcgcc acaacccgtg ccgtaaagag ttgtaatttg agatggcgat gtttcgttgc
     3721 agggcgtcga cgcatgccag gttgagacag aaccgccctc ttcttcaaac tggtcttcgg
     3781 tacgctccat cgatcgtgtg gtgaacccgt caactgatcc tgacagcgga aaattgcggc
     3841 tctgcgcatc atctgcttca gtcgtgttaa ttccactttc catcaggaag ttttctgctg
     3901 ttcgcgttgt tatcgaccag ttacgctcag gtatagagag cccctcgaga actaccggcg
     3961 tataatcagc atttccctga tcccgaaaag ctccacccgc cttggcgatg atgacggcaa
     4021 acttgccggg ctgaaccgtc gtgttaccca ccttcggcac ataatagatc ctcttcacat
     4081 cgccagtcag ctctatgcgc caatcggcaa tatcgatcgg tttattacaa tctgtgttct
     4141 gtatttcgat aaaatcgtcg tctgcatcgt aggttccgtc gttcttcatt gacccggccc
     4201 agttgacctc gcgtatctcg gcgcagccgc gcttgcggtc gacgttgatg gcagtcggcc
     4261 agagttcacc tacggaggtt gtgcagtttg cgcaaagcag cagcgtggct gcgagcatac
     4321 ccaatctgga aattgcgacg tgtcccccca ccgtcctggc ggacacctcc ccccgcatgc
     4381 ggggggagga tgggaggggg gacaatacca taagtttacg catcagaacg acagtgtact
     4441 cctcaggaaa attccccaga aatcggcgtc ggccggcccg ccttttggca ctccattttt
     4501 actgacgata tcttcaatcg gtgtgctgaa aaagctgccg ggcacgaaca tcgcgccggt
     4561 cagctggaac atgagcagtg agttcggaaa gtactgcact gtcaggttga tttcctgccc
     4621 cagcgccttg ccgaaacgtt tttgcgcttc gagttcggcg cgcgagacgt acgggttgtt
     4681 gatcaggtcg aggttattgg ggtcgaagtt cactgcagtc gactgcgtat cctgtacgtg
     4741 ccagatgtcc gcgcgcacaa cgaaactctt ccacatctcg gcaccgatac cagcatgcag
     4801 cagcaacgac cctgccttgc ggttcgcatc gaatggggta tcgtcaatgc ctccgtagtt
     4861 tacataaccc gacgggcgca tgccccagta gcgacgcatc agagtaccgc ccatgcggtc
     4921 gccgcgcatc gagataaatc cgtgcgaaat aatgttaccg ttcttgtcgt acttcgcgcc
     4981 ctgtgagtag aagaagtcaa actctgcgag cggcgcaaga aaacccagca ggctcttggt
     5041 ctgcgcagtc acaccggcgc caaaacccac cgaagcgtaa tcggcaatgg gctgaccctg
     5101 gcgacgcagg tctgacccgg tagaaagagc tgcgtcaccg tagacaatca gctgcttaaa
     5161 gaaggtaaac tggtcgtgca ggtgcgctga aacgcgggcg ccgtagagtc ccgaccagtc
     5221 gttatcagaa aagttgccga ttcggccatt ctctgaatag tcggagccac cccggttacc
     5281 gcgcacgcgc gcgtagtagg catagacccg cgg
//
LOCUS       DAOQJF010000236         5281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9071_length_5281_cov_5.011864, whole genome shotgun sequence.
ACCESSION   DAOQJF010000236 DAOQJF010000000
VERSION     DAOQJF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9071_length_5281_cov_5.011864"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLF85_12005"
     CDS             complement(<1..621)
                     /locus_tag="PLF85_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20438.1"
                     /translation="MWLRFILIAALGTCALPQALIAAPEKKKVVAPAELAAPLVQQTE
                     EAPEKFRPWPLVASIFPGVILHGSGQYVAGNYKAARDLIITEGVSIILVFAVGAPAFA
                     TGNAAVLVPGVYTIGFVGVTHFFTSWAADFYGSSGLNLISSPLVHPHQRTWIAAGIIR
                     QDDTESEIYGLYRFDGKFARENWYVRGLFDIEEKRMYQNFGLDTGYR"
     gene            789..1607
                     /gene="mutM"
                     /locus_tag="PLF85_12010"
     CDS             789..1607
                     /gene="mutM"
                     /locus_tag="PLF85_12010"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNE20439.1"
                     /translation="MPELPEVETVRRSLLATEGQSLQQVKVVDGRLRYPVRAAQFTDL
                     TGRVLQKIDRAGKYLVFNFSAEADEPGVMVIHLGMTGRLLLNAAKNAYSKVEFFFSRD
                     RLVYIDVRRFGFILTGQKAVHALPAGIDALAKDLTKAGERILRSRSPIKNVLLDQKIL
                     SGIGNIYASEILFSAGINPTRMASGLSDGELKRIFTATRKVLQKAIEAKGSSISDFVY
                     ALPGEEKFSTGYYQKQFLVYSREGEKCRRCSATIKKIMQSNRATYVCPNCQKLE"
     gene            complement(1592..2209)
                     /locus_tag="PLF85_12015"
     CDS             complement(1592..2209)
                     /locus_tag="PLF85_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20440.1"
                     /translation="MNPVKLELVKERHDAAGYVHTLFARPAGFEYLPGQYLAITAEGE
                     DKARYLALASHGSEAHLLLLSRHSYEGKSASTSAPQGKGFACDFSAPSPLLFITHGTG
                     ISALRPAILERRARGFTTDTLLFGVADEEHEPDLDVLKNDFPLRQLRAYSRTGDHQRV
                     QQVLPAINANDFAAVLLIGGKEMMSDVKQVLATQGFPAERIYSNF"
     gene            complement(2206..2871)
                     /locus_tag="PLF85_12020"
     CDS             complement(2206..2871)
                     /locus_tag="PLF85_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20441.1"
                     /translation="MPVLSCVVDHYHQIVVNTHVRRFHTTYAEYFNEKSTRALADYFF
                     EKIYTLEGKSERDELAVKTYNRFKGMLSEKSRERIENLLLLNEITDELDSQMADVIRK
                     DKKYIVKHDGIEHVDLKKVHMLYRKANDPEARIRQLQLIILNLESFFELSKHPLAGVV
                     MRPVSLAARTVGAIKLYRIFEEGYNATKPVSKDVFFEFTQYVRGHETQFLEKAYKRHI
                     HLL"
     gene            3057..4166
                     /locus_tag="PLF85_12025"
     CDS             3057..4166
                     /locus_tag="PLF85_12025"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNE20442.1"
                     /translation="MFCVLFLPGWFGVSAAVAKKAPGKEKIRLTLVQIDKLPYLSAAN
                     LRRIHNSLELRYVPKGEQGEIRFGRRVLLFNLNEAQYQSQNEIRELDEPGAIDDDGLF
                     FSREFVEEILSELNVPVAYRFDRNNLIIVRATARHTRPTLDFVVIDAGHGGKDSGALG
                     YFDAAEKDLTLSLAHAVRKQLASDYPELKVYMTRSTDKFLRLERRSELANKQNGSAKF
                     GVFISIHCNSTLSPKVKGFEVYYLAQNPDNTRTRQIMLRENLRYERSSYIKRLKSQLM
                     NAQIQRESKTLARGVFSGLMNGLDGMIKPRKVKKADFAVLRGSLMPAILIETGYLTNR
                     DDLKMLASENYRKAFAQGVSKGIGAFLGDLAKTEK"
     gene            4141..4845
                     /locus_tag="PLF85_12030"
     CDS             4141..4845
                     /locus_tag="PLF85_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20443.1"
                     /translation="MIWQKLKNSSMDLVAALASRINTVVAFLAKPLNLFRFIVILLAL
                     DFVTFMSLTRSSYLQLVNPLHFLWAEPGESRKSIELYFPRSLSLTGLEKIYPDEDTSQ
                     GKKTVAEQSAQEKPLDDAAVAQEALLTRKLVSEPVTKFGELQLSKEEALARRVILELI
                     AGPAGELETLKARNLLKEPLFLRSLWTREGTLYISTEKSLWDRMSPNERKVTEFCIRE
                     SLKKNLGETRFALLKE"
     gene            4854..>5281
                     /locus_tag="PLF85_12035"
     CDS             4854..>5281
                     /locus_tag="PLF85_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNE20444.1"
                     /translation="MKTQEIEAAAAFLRTKVNFPIQVAVVLGSGLSAFAERLEKAVAV
                     PYGTVPNFPQSTVPGHSGRFVAGFLEDTSVLCMQGRVHFYEGYSMQQVSLGVRLMASL
                     GVRTLILTNAAGAVNTAYKPGDFMLLSDHLNFMGTNPLIGT"
BASE COUNT         1198 a         1409 c         1494 g         1180 t
ORIGIN      
        1 acggtaaccc gtgtcgaggc cgaaattctg gtacatgcgc ttttcttcga tatcgaaaag
       61 gccgcgcacg taccagtttt cgcgcgcgaa ctttccgtcg aagcggtaga ggccgtagat
      121 ttccgattcc gtgtcgtcct gccggatgat acccgcggca atccaggtac gctggtgcgg
      181 gtgtaccagc ggcgacgaaa tcaggttcaa cccgctcgag ccatagaaat ctgcggccca
      241 gctggtgaag aagtgggtaa ctcctacaaa gccgatggtg tacacgcccg gcacgagcac
      301 cgcagcatta ccggtagcaa atgccggcgc acccacggca aatacgagga tgatcgaaac
      361 accttctgta atgatcaggt cgcgcgcagc cttatagttg cccgcgacat attgtcccga
      421 accatgcaga atgacgcctg gaaagatcga agcgaccaag ggccaggggc gaaacttttc
      481 cggggcttct tcggtttgct ggacgagtgg cgcggcgagt tcagcgggag cgacgacctt
      541 ctttttctcc ggggccgcaa taagagcctg ggggagtgcg caggtgccga gggcagctat
      601 caggataaaa cgcagccaca taaatgtccg ggtaatttat caaacacctt tcgcttcggc
      661 tacgtaagtt gcaggcaatc tatggacaag ggcttagggg tgagcggctg gtcgggtacg
      721 cgaccagccg ctcaccccat ctctttcaaa ttcgcgtttc tgcctgacac ggcgcccggt
      781 actggcccat gcccgaactg cccgaagtgg agacagtccg acgttcgctg ctcgctaccg
      841 aggggcagtc actgcaacag gtcaaagttg tggatggacg gctgcgttac ccggtgcggg
      901 cagcgcagtt tactgatctg acgggcaggg tgctgcaaaa aattgaccgt gctggcaaat
      961 acctggtgtt caatttctct gccgaagcag atgagcccgg tgtgatggtt atccacctcg
     1021 gcatgacggg gcggctgttg ctcaatgccg cgaagaatgc ttacagcaag gtggagtttt
     1081 ttttctcacg cgaccggctc gtgtatatcg atgtgcgccg ctttggtttt atcctgaccg
     1141 ggcaaaaagc cgtgcatgct ctaccggcgg gtatagacgc ccttgccaaa gatcttacga
     1201 aagcaggtga aagaattctg cgcagccgtt caccgatcaa gaatgtcctg ctcgaccaga
     1261 agatactgag cggcatcggc aacatctacg cttcggagat acttttttcc gccgggatta
     1321 atcccacgcg catggcttcg gggctaagtg acggtgaact gaagcgaatc ttcaccgcga
     1381 cgcgtaaggt actgcagaag gcgattgaag ccaagggtag cagcatttct gactttgtct
     1441 atgcgctgcc cggcgaagaa aagttcagca ccggttacta ccagaaacag tttttggtct
     1501 actcgcgcga gggtgaaaaa tgccggcgct gctctgccac aataaagaaa atcatgcagt
     1561 cgaaccgcgc gacctatgtc tgccccaact gtcaaaaatt ggagtagatg cgttctgcag
     1621 gaaaaccctg cgtggcaagc acctgcttca catcgctcat catctctttg ccgccgataa
     1681 gcagtacggc ggcaaaatcg ttggcattga tcgccggcaa tacctgctgc acgcgctggt
     1741 gatcacccgt gcgcgaatag gcacgcagct gccgcagcgg aaaatcattt tttagaacat
     1801 cgagatcggg ttcatgctct tcgtcggcca cgccgaagag cagtgtatcg gtggtgaaac
     1861 cgcgcgcgcg ccgttctagt atcgccggcc tcagcgcaga aatgcctgtg ccgtgagtga
     1921 tgaagagcag ggggctgggt gccgagaagt cacaggcgaa ccctttgcct tgcggagccg
     1981 atgtactcgc ggatttaccc tcgtaactgt gccggctgag cagcagcagg tgcgcttcgc
     2041 tgccgtgcga ggccagcgcc aggtagcgcg ccttgtcttc accttctgca gtgatggcca
     2101 ggtattgccc aggaagatac tcaaaacccg ccggccgcgc gaacagtgta tggacataac
     2161 ccgcagcatc gtgccgttct ttgacgagct caagttttac aggattcata acaggtgtat
     2221 atggcgcttg taggcctttt caaggaactg ggtttcgtgg ccgcgcacgt actgcgtgaa
     2281 ctcgaaaaag acatctttgg agacaggctt ggtggcgtta tatccctctt caaatatgcg
     2341 gtagagtttg atcgcgccga cggtgcgggc agcaagcgat accgggcgca tgaccacgcc
     2401 tgcgagcggg tgtttgctca gttcaaaaaa cgactcgagg ttgagaataa tcagctgcag
     2461 ctgcctgata cgcgcttccg ggtcgttggc tttgcggtag agcatgtgga ccttcttcag
     2521 atcaacgtgt tcgatgccgt catgtttgac gatgtacttc ttgtccttac ggatcacgtc
     2581 tgccatctgg ctgtcgagtt cgtcggtaat ctcattgagc agcagcaggt tctcgatgcg
     2641 ttcgcggctc ttctccgaca gcattccttt aaagcggttg taggttttca ctgccagctc
     2701 gtcccgctct gattttccct caagcgtgta gatcttttcg aaaaagtaat cggccagcgc
     2761 acgcgtcgat ttctcgttga agtactctgc gtaggtagta tggaagcgcc gtacatgcgt
     2821 attgacgacg atttggtggt aatgatcgac cacgcaggac agaactggca tcggaaaaag
     2881 cgggtcaacc catctaacag ggtcctgaaa actgagccag aagcaggttc tgaggctgac
     2941 attgcagaaa cagctttgca cgagtgctcg cttcgcgtaa ctgtcggcga ttgttaaccc
     3001 gggtttcggc agtcgctatg tcattccggc ctgattgcgg gctgcggatc tggtttgtgt
     3061 tctgtgtcct gtttctgcct ggctggttcg gtgtatctgc cgctgttgcg aaaaaagccc
     3121 caggaaaaga aaagatccgg ctgacgctgg tgcagattga taagctgccg tacctgtccg
     3181 cagcaaacct gagacgcata cacaatagcc ttgagctgcg ttatgtaccc aaaggtgagc
     3241 agggcgaaat ccggtttggc aggcgcgtac tcctgttcaa cctgaatgag gcgcagtacc
     3301 agtcacagaa cgaaatcagg gagctcgatg agcccggcgc aattgatgat gatggattat
     3361 ttttttcccg tgaatttgtc gaagaaattc tgagtgaatt gaacgtcccg gtggcctacc
     3421 ggttcgaccg caataacctc attattgtcc gcgctactgc gcgtcatacc cggcccacgc
     3481 ttgattttgt cgtcatcgat gcaggtcatg gcggcaagga ttcaggcgcg ctgggttatt
     3541 tcgacgcggc cgagaaagat ctcaccctgt cccttgcgca cgccgtcaga aaacaacttg
     3601 cctctgatta tcctgagctg aaggtctaca tgacccgcag cacggacaag ttcctgcgcc
     3661 tggagcgaag aagtgagctg gccaataaac aaaacggtag cgcaaaattc ggcgtcttca
     3721 tcagtataca ctgtaactcc accctgtcgc cgaaggtgaa aggctttgaa gtctactacc
     3781 tcgcacaaaa ccctgacaac acacgcacgc ggcagattat gcttcgcgag aacctgcgtt
     3841 atgagcgcag cagttacatc aagcggctga agagccagct gatgaacgcg cagatccagc
     3901 gggaaagtaa aacccttgcc cgcggcgtgt tttcaggcct gatgaatggc cttgacggta
     3961 tgattaaacc gcggaaagtg aagaaggcgg attttgcggt tttgaggggt tccttgatgc
     4021 cggcgatcct gattgagaca ggttacctga cgaacaggga tgacctgaaa atgctggcca
     4081 gcgaaaacta ccgaaaggcg tttgcgcagg gtgtcagcaa aggaatcgga gcgtttttgg
     4141 gtgatctggc aaaaactgaa aaatagttca atggacctgg tggccgctct ggccagccgc
     4201 atcaataccg ttgtcgcgtt tctcgcgaaa ccgctgaacc tgttccggtt tattgtcatc
     4261 ctgctcgcgc tcgattttgt caccttcatg tcgctcaccc gcagcagcta cctgcaactg
     4321 gtgaacccac tccatttcct gtgggcagag ccaggcgaat cgcgcaagag cattgaactg
     4381 tattttccgc gctcgctctc gctgactggt cttgagaaga tataccccga tgaagatacg
     4441 agccagggta agaagacggt ggctgaacaa tcagcgcagg aaaagccgct cgacgatgcg
     4501 gctgttgcgc aagaagccct gctgacccgt aaactggtgt ctgagccggt cacaaaattt
     4561 ggcgagcttc aacttagtaa agaagaagct ctggcgcgcc gggtcatact cgaactcatt
     4621 gcaggtccgg ctggggaact cgaaaccctg aaagcccgta acctgctcaa agaacccctt
     4681 ttcctgcgct cgctctggac gcgcgaaggt acgctctaca tctccacaga aaaaagtctc
     4741 tgggatcgta tgagcccgaa cgaacgcaag gtcaccgaat tctgcatccg tgaatcactg
     4801 aaaaagaacc tgggcgaaac ccgatttgca ctgctgaaag aatgatgcaa caaatgaaaa
     4861 cgcaggaaat cgaggccgca gcagcatttt tgcggaccaa ggtgaatttt ccgatccagg
     4921 ttgccgttgt gctcgggtca ggcctttctg cttttgccga gcgcctggaa aaagccgtcg
     4981 cggtaccgta cggtacagtg cccaattttc cgcagtcaac ggtgccgggc catagcgggc
     5041 gttttgtcgc cggttttctt gaagacacct ctgtactgtg catgcaggga cgcgtgcact
     5101 tctatgaagg gtactcgatg cagcaggttt cgctgggcgt gcgcctcatg gcttcgctgg
     5161 gcgtgcgcac cctgatcctg acgaatgccg ccggcgcggt caacaccgct tacaagccgg
     5221 gggattttat gctgctcagc gaccatctca acttcatggg caccaacccg ctgattggta
     5281 c
//
LOCUS       DAOQJF010000237         5275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9090_length_5275_cov_4.547126, whole genome shotgun sequence.
ACCESSION   DAOQJF010000237 DAOQJF010000000
VERSION     DAOQJF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9090_length_5275_cov_4.547126"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLF85_12040"
     CDS             complement(<1..1192)
                     /locus_tag="PLF85_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE20445.1"
                     /translation="MLESLTVKDFALIRELNIQFGPGLNLITGETGAGKSVMLGALSL
                     VLGYKATTDMIRSGSKRAIVEAAFAMPPDTRSGARALRQVLADQGLDDDEGSLILRRE
                     ISVEGKGRSFVNARQVPSSVLRDIGRFLVDIHGQNEHQNILTVEQHRSILDRYAHLGE
                     LVAEMRKLHRAREEARQKLSSVTLSEDDKARRLDILQHEIAELEAARIDDVREIDELI
                     AREKSLANAETLVSDLNSVYEALSGEGGFVPRGGSIERMLETDSQYDESLAAILTQFR
                     ESYYALEEIARQVRSKAESYSLNPAELEEMRERIGLLQSVARKYGGRGPAGAKEGDYA
                     VGLEKAKAYLEKAKNELTGIELSSGEEARLRKEIDSLNAQMKDKALYLSEKRRLASLE
                     LEQKV"
     gene            1255..1668
                     /locus_tag="PLF85_12045"
     CDS             1255..1668
                     /locus_tag="PLF85_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20446.1"
                     /translation="MSQLWHSAVKFLDIDIHKPDFQTLKAALELLADDYVILFPADLG
                     LSVVCKAAPPARTKFSAIVGSPENLRLAVAEDMDLAKLAQGDITPYLPDYDTNRAFHT
                     LVQLCDFALLVSPTALDRATADKAYPGIEYGFWAL"
     gene            1726..2748
                     /gene="plsX"
                     /locus_tag="PLF85_12050"
     CDS             1726..2748
                     /gene="plsX"
                     /locus_tag="PLF85_12050"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNE20447.1"
                     /translation="MSSGSSEQWMAVDAMSGDHGPGPLVLGAINAAHELNAHVLLVGD
                     EKILQTELKRHGYSGTNIRVVHASDVITMEDSPAMAVRQKKDASVNVAARLVANGEAL
                     GFFSPGNTGASMAAALAHMGRLPGVKRPAIATPIPREDGGATVLLDSGANVDCKPEYL
                     VQFAIMGEVYSREIMGVINPKIGLISNGEEDTKGNEMTLAALNKLRKLPYDFVGNVEG
                     RDLYGTGKPVNVVVCDGFVGNVILKSIEGLAKTIFSSLKKNINASAIAQGGALLMKPA
                     FLAVKNRMDYATYGGAPLLGVAGVCIIGHGSSSARAVRNAIRVGLEFGQKNVTGHIAS
                     NIKRFA"
     gene            2763..3128
                     /locus_tag="PLF85_12055"
     CDS             2763..3128
                     /locus_tag="PLF85_12055"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNE20448.1"
                     /translation="MHISLLRAKVHRAEITDANLFYEGSLTLDEDLMDAAGLMEYEKV
                     HVVNVNNGARLETYLIKGKRGGHEVCLNGAAARLGHRGDKIIIIAYAQMTPEEAKSFT
                     PKIVLLGEGNSIQKILPAV"
     gene            3334..4260
                     /locus_tag="PLF85_12060"
     CDS             3334..4260
                     /locus_tag="PLF85_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE20449.1"
                     /translation="MNKKDLLLLTAVTLHCGTYRPQTQALPAQQSQKLAETQLFKPAT
                     RNLPQLVTLNTGSVEVDTSLLLDIEKHPQFKNTKTHVPVLAHLVRHPQKGDILIDTGF
                     DASFARSGHGNFGCMGVFFDFARQNPGSDIAAQLLAHNAKLTAVYFTHMHVDHTAGAP
                     ALAKDIPYIAGKGTLADSYADIFGLCMKHLDGIETIKEIDFSAQPEVAGVGRVFDIFG
                     DGTVWAIHTPGHSAGHMSFLVNAKEGAYLVTGDASHTAYGFQNNMAPGKMHDHAQGEQ
                     TLATLRALAARHPQMKLVFGHDPEQNYAPVKK"
     gene            4263..4823
                     /locus_tag="PLF85_12065"
     CDS             4263..4823
                     /locus_tag="PLF85_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HNE20450.1"
                     /translation="MRRGLNRDALLDAAIKIGDRGGFTAITVGTLAAALKIKPPSLYK
                     HVAGIDDIVDGIGIRGANMLVADLSPLRDGKNPQRAFLRACEAYRAFATKNPTCYAAL
                     QPAMARRSPGFQLAAGELLKAIFSIVSDLGVPQKELVHAVRALRSMLHGFVTLQLVGG
                     FGMPEDIETSFRKMLEGFLIAHAAGR"
     gene            complement(4862..>5275)
                     /locus_tag="PLF85_12070"
     CDS             complement(4862..>5275)
                     /locus_tag="PLF85_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20451.1"
                     /translation="PLLGLIMVIFPLYGNVRRLRLMRYGQVAQGRLEKAEDTNMSVDE
                     ETVVRFRFVFEDTQGRQHRLVYRTHETERITDDKKEAILYFAHKPAVACLVDALPGNT
                     KLQGDGVVQFHAAFSWNDLLAPVMIVLALLPAFFV"
BASE COUNT         1167 a         1551 c         1448 g         1109 t
ORIGIN      
        1 gcactttttg ctcgagctcg agcgaagcga gccggcgctt ttcagagagg taaagcgcct
       61 tgtctttcat ctgcgcgttg agcgaatcga tttctttgcg caggcgcgct tcttcacccg
      121 acgagagttc gatgccggtg agttcgttct tcgctttttc gaggtatgcc ttcgcctttt
      181 cgaggcccac cgcgtagtcg ccctctttgg cgcctgccgg gcccctgccc ccgtacttgc
      241 gcgccaccga ctgcaagagc ccgatacgct cgcgcatctc ttcgagctcg gcggggttta
      301 gcgaatatga ctcggccttc gagcgtacct ggcgcgcaat ctcttcgagc gcgtagtaac
      361 tttctctgaa ttgcgtcaga atcgccgcga gcgattcatc gtactgcgaa tcggtttcga
      421 gcatacgctc gatcgaaccg ccgcgcggca caaagccgcc ttcgccgctc agcgcttcgt
      481 agacggagtt caggtcgctg acgagcgttt cggcgttcgc gagacttttc tcgcgggcaa
      541 tgagttcatc aatctcgcgc acatcgtcga tgcgcgcggc ttcgagttcg gcaatttcat
      601 gctgcagaat gtcgaggcgg cgcgccttgt cgtcttcgct cagcgtgaca ctcgacagct
      661 tctggcgtgc ctcttcgcgc gcccggtgga gcttccgcat ttctgcgacc agttcaccga
      721 ggtgcgcata gcgatcgaga atcgaacggt gctgctcaac agtgagaata ttctggtgct
      781 cgttctggcc atggatatcg acaagaaagc ggccaatatc gcgcagcacc gaagaaggaa
      841 cctggcgcgc gttaacgaag ctgcgcccct taccctcgac gctgatctcg cggcgcagaa
      901 tgagagaacc ctcgtcgtcg tcgagaccct ggtcggccag cacctgccga agcgcgcgcg
      961 cgccgctgcg ggtatcgggg ggcatcgcaa aggctgcctc gacgatcgcg cgcttagaac
     1021 cgctgcgtat catgtcggtc gtggctttgt agcccaaaac cagggagaga gctcccagca
     1081 tgactgattt gcccgcgccg gtttcacccg tgatcaggtt gagccccggc ccaaactgga
     1141 tgttcagctc gcgtatgagt gcgaaatcct taactgtcag agactcgagc atgggcgcct
     1201 tgctgattgt ctcagatcac gacggagcga aaagagtttt tcgttgtgca gagtgtgagc
     1261 caactctggc atagcgccgt gaagtttctc gatatcgaca tccacaagcc ggatttccag
     1321 accctgaagg ccgcgctcga gcttttagcc gatgactacg tgatcctgtt cccggcagac
     1381 ctggggttat ctgtcgtctg caaagccgcg ccgccggccc ggacaaagtt ttctgcaata
     1441 gtgggatcgc cggaaaatct gcgccttgcc gtggcggaag atatggacct ggcaaagctt
     1501 gcccaaggcg acataactcc ctatttgccg gactacgata ccaaccgggc gtttcacaca
     1561 ctggtacagc tctgcgactt tgctctttta gtatcaccga ccgccctcga ccgtgcaaca
     1621 gcggacaagg cttaccccgg tatcgaatac ggcttctggg cgctgtagcc tgcccaggta
     1681 tgtgaaaggg cttggcaagg tgagtttgag acaagagtcg aacgtatgag ttcaggctca
     1741 tcggaacagt ggatggccgt cgacgcgatg agcggagacc acggcccagg tcccctcgtg
     1801 ctgggcgcaa tcaatgccgc gcacgagcta aacgcacacg ttttgctggt cggtgacgaa
     1861 aaaatcctgc agaccgaact caagcgccac ggttacagcg gcacgaacat ccgcgttgtc
     1921 catgcgagcg acgtgatcac catggaagat tcgccggcca tggcggtgcg gcagaagaaa
     1981 gatgcctcgg tgaatgttgc cgcgcggctt gtcgccaacg gcgaagccct gggctttttc
     2041 tcaccgggca ataccggcgc ctcgatggca gccgccctcg cccacatggg tcgcctgccg
     2101 ggtgtcaaac gccccgcgat cgccaccccc ataccacgcg aagacggagg cgccacggtg
     2161 ctgcttgatt caggcgcaaa tgtggactgt aagcccgagt atttagtaca attcgcgata
     2221 atgggcgaag tctactcgcg cgaaatcatg ggggtcatca atcccaagat cggccttatt
     2281 tccaacggtg aagaagatac caagggcaac gagatgacgc tcgctgcgct caataagctg
     2341 cgcaagctgc cttatgattt tgtcggcaac gtcgagggcc gtgacctgta cggtaccggc
     2401 aaacccgtca acgtcgtggt ctgcgacggc ttcgtcggca acgtcattct gaagtcgatc
     2461 gaaggtcttg cgaagacgat tttctccagt ctgaaaaaaa atatcaatgc ctctgccatc
     2521 gcacagggtg gcgcactgct gatgaaaccc gcatttctcg cggtaaaaaa ccgcatggac
     2581 tacgcgacct atgggggagc tccccttttg ggggtcgcgg gcgtctgcat tatcggccat
     2641 ggctcttcga gtgcgcgtgc ggtgcgcaac gccatccgcg tcgggctcga attcgggcaa
     2701 aaaaacgtta cagggcatat cgccagcaat atcaaaaggt ttgcctaaat cggagcttaa
     2761 gcatgcacat ttcacttctc agagcaaagg tccaccgggc cgaaatcacc gacgcgaacc
     2821 tcttttacga aggcagcctg accctcgacg aagacctgat ggatgctgca ggcctcatgg
     2881 agtacgaaaa ggtgcacgtg gtcaacgtca acaacggcgc gcggctcgag acctacctga
     2941 tcaagggcaa acgcggtggc catgaagtct gcctcaacgg cgctgcagcc cgccttggac
     3001 accgcggcga caaaatcatc atcatcgcct acgcgcagat gacacctgaa gaggcgaagt
     3061 cttttactcc gaagattgta ctgctcggcg aaggcaatag catccagaag attttaccgg
     3121 cagtataagc ggccggttct tagcctttcg ggcctgtcaa cccggaagat gaattattct
     3181 tcctttcact atccatcgat atactgaaac ccggacaccg cttctccttt tacatacggt
     3241 cagcaaaacg ggcgaacaat aaaaacagaa aactctttac aagctaattg tattagcata
     3301 aatagctaat acaattagcc aggaggtaca accatgaaca aaaaagatct tctattactc
     3361 actgcagtca ccctgcactg cggcacctac cgcccgcaaa cgcaggcgct tccagcgcag
     3421 caatcgcaaa agctggcaga gacacagcta ttcaaacctg cgacgcgcaa cctgccgcag
     3481 cttgtgactc tcaataccgg cagcgtcgaa gttgatactt cgctgctgct cgacatcgaa
     3541 aagcacccgc agttcaagaa caccaaaacc catgtgccgg tactcgcgca cctcgtgcgg
     3601 catccgcaga aaggcgacat cctgattgat acaggcttcg acgcttcttt cgccaggtcg
     3661 gggcacggca atttcggctg tatgggagtc ttttttgatt ttgcccggca aaacccaggc
     3721 agcgatattg ccgcacaact gctggcgcat aatgcaaaac tgacagctgt ctacttcacc
     3781 cacatgcacg tcgatcacac agcaggagct cccgcgctgg cgaaagacat accgtacatc
     3841 gccggcaaag gcaccctcgc tgactcttac gccgacatct ttggtctctg catgaaacac
     3901 ctcgatggta tagagaccat taaagaaatt gatttctccg ctcaacccga agttgccggt
     3961 gtaggccgcg tctttgatat tttcggcgac ggcacggtgt gggctattca cactcccggt
     4021 cattccgcag gccatatgtc tttcctggtc aatgccaaag agggagctta tctcgttacg
     4081 ggagacgcca gccacacggc ctatggattc cagaacaata tggctcccgg caagatgcat
     4141 gaccacgcac agggagaaca gacactcgct acgctgcgtg cactcgcagc gcggcatccg
     4201 caaatgaagc tggtgttcgg ccacgaccct gaacagaact atgcaccggt aaaaaaatag
     4261 ccatgcgccg cggtctgaac agagatgccc tgctcgatgc ggcaattaag atcggcgatc
     4321 gtggcggttt caccgccatt accgtcggta cgctggcagc ggcgctcaag atcaaaccgc
     4381 cgtcgcttta caagcatgtc gcgggcatcg acgacattgt cgacggcatc ggcatccgcg
     4441 gcgcgaatat gctggtggca gatttgagcc cgctgcgtga tggcaagaat ccccagcggg
     4501 ctttcttgcg ggcatgcgag gcataccgcg catttgccac caagaaccct acgtgttatg
     4561 ccgccctaca gcccgccatg gcaaggcgct ctcccggttt ccagctggcc gccggcgaat
     4621 tgctgaaagc catcttcagc atcgtttcag atctgggcgt cccacagaaa gagctcgtgc
     4681 atgctgtgcg ggcgctacgc agcatgctgc atggcttcgt gacactgcag ctcgtcggtg
     4741 gctttggtat gccggaggat attgagacga gcttccgcaa gatgctggaa ggctttctca
     4801 tcgcccacgc ggcaggccgt taaccgtatc ggatagtttt ttccgcgtac gccgctcaca
     4861 atcaaacgaa aaaagccgga agcaaagcga gtacaatcat gacgggggcc agcaggtcgt
     4921 tccagctgaa agccgcatgg aattggacca ctccgtcacc ctgtagtttt gtgttacccg
     4981 gcagtgcatc gaccaggcag gccactgccg gcttgtgtgc gaaatagaga atagcctctt
     5041 tcttatcatc agtgatgcgt tcggtttcgt gcgtcctgta cactaggcgg tgttgcctgc
     5101 cctgggtatc ttcaaaaaca aaacggaagc gtacgactgt ctcttcatcg accgacatgt
     5161 tggtatcttc agccttttcc agccggccct gtgcgacctg gccgtagcgc attagccgga
     5221 gcctgcgaac attgccgtat aggggaaaga tcaccatgat cagtccgagc agcgg
//
LOCUS       DAOQJF010000238         5261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9128_length_5261_cov_5.401460, whole genome shotgun sequence.
ACCESSION   DAOQJF010000238 DAOQJF010000000
VERSION     DAOQJF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9128_length_5261_cov_5.401460"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PLF85_12075"
     CDS             <1..1288
                     /locus_tag="PLF85_12075"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE20452.1"
                     /translation="KMRKGDFFSVGPWRRMALAFGGPLFSVLLGFLVVLFLLQTGYRP
                     MDNTVRVDTKSALYKAGLRDGDRVVQVDGAKTPSFDRIIYEFGFAHSDTVDVTVERNS
                     VPVPLRLPVVRSEPGTPGMFTGARPLSRLTMTEAGPKGTGLNKGDEILQANGVELFSI
                     EDLIRVLNDKPADSAELLVHRHEGGAAKQLTLTVPLKKVPRIILKNLRDVQTGKNLPE
                     IVIEEGAGNEFQNIFINGESFRTFAEFRAALENSETTPKLRAGPVEISGTIEPGFRRQ
                     IGIRPAEKAISSLSEMSFVSKIILSFDETVFITKSTLVGLGRIIEGKLSFNKSVSGPV
                     KMVAIAAKTVELGWQTYFLLMAQITIILGVMNLLPIPVLDGGHIIFYLIEAIYKPVSF
                     KVQATATRIGMALMLAMGIYVIGLDIIDVFFRRVFG"
     gene            1291..1848
                     /locus_tag="PLF85_12080"
     CDS             1291..1848
                     /locus_tag="PLF85_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20453.1"
                     /translation="MPRKALQKKHQKLLSHSSHVGLAIDGYDDLFSDFDPRPYHEREL
                     SEDFLTELRRFFYHKSAEYLDIVLLVPAKRRRARDEDIVKKRLHIYFQKKYRETKKSL
                     KHTTLWNLLRIFLAMSIMILTGFLAVHAGQVLWRNILKVMLEPASWWLFWTSFDNLLA
                     ARKKVNDELQYFHRLAECKIVFLPR"
     gene            complement(1878..3170)
                     /locus_tag="PLF85_12085"
     CDS             complement(1878..3170)
                     /locus_tag="PLF85_12085"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNE20454.1"
                     /translation="MQYFLLVLFFLGLSVFIGKQIPWFSRYRIPPSLTAGAIALLLFT
                     AIPGAKNSDFFLHLKQLPSEFIALVFACFFLQRHENTGLSERRITDVFAQTSLIWVSV
                     MGQVLIGLVATILVYKPFFGQPLAFTSVLEAGFAGGHGTAVAMGPILVQNGVPAGLEY
                     ALFSATVGLIAGIAGGVWLIERQRRKTRMIVSDEDLVHAQLDVTGLLTSLGLVAAAYW
                     LGLFYKGVFEKEILPRITDPAHLGSFSLPLFAYTLIGGYTVKQLLTLAGFSRLIDNNS
                     ILLLADVFLEILIFAGIAIIDVQLLGQGLIPLLSLFAMGFAWNLFCHIWLRPRILNKA
                     YSFELGLINFGMLNGTAATGLMLLKMVDPKFQTPAAKVFAESSAITQPFIAGGILTLA
                     TPYIVSQLSPALSVLLYSGLLAVWLVLGLAAGKRIRNR"
     gene            complement(3247..>5261)
                     /gene="uvrA"
                     /locus_tag="PLF85_12090"
     CDS             complement(3247..>5261)
                     /gene="uvrA"
                     /locus_tag="PLF85_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNE20455.1"
                     /translation="CSGLGHMLEFHPDRLIRDDEQTVGDGLGDGLGFSGDAYWYKASI
                     DALRKKVAFDPKTPWRKLPKKIFDLLLYGDKSLKLNYEWKGGDSTYQFSRGYEGIIPN
                     LHRRYMQTQSETMRQKMEQYMEHMPCPACKGKRLKPVALAVRLKGKNIAETTAMTLEG
                     ALDYFQKIQFNKTEMEIAKQAMKEIFDRLRFLNSVGVGYLTLDRIAGSLSGGEAQRIR
                     LATQIGSALMGVLYVLDEPSIGLHQSDNAKLIATLKGLRDLGNTIVVVEHDEETMQES
                     DYIVDMGPGAGRHGGQVVFAGTYAQLTKAKNSLTADYLTGRKQIEIPKERRKPRKEKI
                     RIVGAQENNLKNIDVEFPLSVLTVVTGLSGSGKSSLVNEILFKGINRQLMGAQDLPGK
                     HKSITGLEHIDKIINIDQSPIGRTPRSNPATYTGAFGPIRDLFAALPASQMRGYKAGR
                     FSFNVEGGRCEACSGDGVKKIEMHFLSDVYVKCEVCHGKRYNQETLEVRFKNKNIYEV
                     LEMPVEEAAVFFEAIPAVHTKLKALNDVGLGYVRLGQAATTLSGGEAQRVKLATELSR
                     RSTGKTLYILDEPTTGLHFEDIRLLMNILQRFVDEGNTVVVIEHNMDVIKCADYIIDM
                     GPEGGVKGGQVVATGTPEEIASQKNSLTGEFLKRWLKPAGPAKTRR"
BASE COUNT         1161 a         1433 c         1472 g         1195 t
ORIGIN      
        1 caagatgcgc aaaggtgatt tcttctcggt cggcccctgg cggcgtatgg cactcgcctt
       61 cggtggcccg ctcttttctg tcctgttggg atttctcgtg gtcctgttcc tcttgcagac
      121 gggctatcgc ccgatggaca acaccgtccg ggtcgacacg aaatctgcgc tctacaaggc
      181 aggtctgcgc gacggtgacc gcgttgtgca ggtggatggc gcgaaaacgc cttcatttga
      241 tcgtatcatc tacgaatttg gtttcgcgca cagcgacacg gttgatgtga ccgtcgaacg
      301 caactcggta ccggtgccac tgcgtctacc cgtcgtgcgc agcgaacccg gtacgccggg
      361 gatgttcacc ggggcgcgac cgctttcgcg cctgacgatg accgaagcgg ggccaaaggg
      421 caccgggctc aacaaaggcg atgaaattct tcaggcaaac ggggtggagc tcttctcgat
      481 cgaagacctg attcgtgtgc tcaacgataa acccgccgac agcgccgagc tgctcgtcca
      541 ccgtcatgaa ggtggcgccg cgaagcagct cacgctgacc gtgccattga agaaggtgcc
      601 ccgcattatc ctgaagaacc tgcgcgacgt acaaaccggc aagaacctgc cggaaattgt
      661 gattgaagaa ggagctggca acgagttcca gaacatcttt attaatggcg agagcttccg
      721 tacttttgcc gagttccgcg ctgcgctgga aaattcggaa acgacgccaa agctgcgcgc
      781 aggtccggta gaaatctcgg gtaccatcga acctggcttt cgccggcaga ttggaatccg
      841 tcctgcagag aaggcgatca gctcgctcag cgaaatgtca ttcgtgagca agattatact
      901 cagtttcgat gagactgtct ttatcaccaa gtctactctg gttggcctcg ggcgcatcat
      961 cgagggtaag ctctcgttta acaagagcgt gtcgggcccg gtcaagatgg tggcgatcgc
     1021 tgccaagacg gttgagctcg gctggcagac ctactttctg ctcatggcgc agattacgat
     1081 cattctggga gtgatgaacc tgctgcctat tcctgtactc gacggcggcc atattatttt
     1141 ctacctgatt gaggcgatct acaaaccggt ttcattcaag gtacaggcca ctgcaacgcg
     1201 tataggtatg gcgctgatgc tagcgatggg tatctacgtg attggactcg atatcatcga
     1261 cgtgttcttc cgccgcgtgt ttggctgaaa atgccgcgca aggccttaca gaagaaacac
     1321 cagaaactgc tctcgcatag ttcgcatgtc ggccttgcga tcgatggata tgacgacctg
     1381 ttttccgatt ttgacccgcg accgtaccac gaacgcgagt tatctgaaga ctttttaact
     1441 gaactccgcc gcttcttcta ccataaatca gccgagtatc tcgatatcgt gctgctggtg
     1501 ccggcaaaac gccggcgagc ccgtgacgag gatattgtca agaagcggct gcatatctat
     1561 ttccagaaga agtatcgcga aactaaaaaa agcctgaagc acaccacact gtggaaccta
     1621 ctgcgcattt ttctcgctat gtcgataatg attctgaccg gctttcttgc cgtacacgcc
     1681 gggcaggtgc tctggcgcaa catactcaag gtaatgctcg aaccggccag ctggtggctc
     1741 ttctggacga gctttgataa tctactggcc gcacgcaaga aagtgaacga tgagctgcag
     1801 tattttcacc gcctcgcgga atgcaagatt gtctttctgc cgcgctgaaa agcggcgtta
     1861 gacctgaccc gggcgagtca gcgattacgg attcgtttgc ccgctgcaag ccccagcacc
     1921 agccagaccg ccagcagacc tgaatagagc aacaccgaca atgccggcga gagctggctc
     1981 acaatgtacg gggttgccag cgtgagaatg ccaccggcga taaagggttg cgtaatggca
     2041 gaactttcgg cgaagacttt cgcggcagga gtctggaatt tcgggtcaac catcttgagc
     2101 agcatcaagc cggtagccgc ggtaccgttg agcataccga agttgatcag gccgagttca
     2161 aaactataag ccttgttcaa tatgcgcggt cgcagccaga tatgacagaa aagattccag
     2221 gcgaagccca tcgcgaagag gctcagcagt ggtattaagc cttgcccgag caactgtacg
     2281 tcgatgattg caatacccgc gaagatcagg atctccagga agacgtcggc gagcagcaag
     2341 atgctgttgt tatcgatcag ccgggagaaa cccgcaaggg taagaagttg ttttactgta
     2401 taaccaccga tcagcgtgta cgcaaagagc ggcaggctga agctgccgag gtgtgcgggg
     2461 tcggtgatgc ggggtaatat ctctttctca aagactccct tgtagaaaag accgagccaa
     2521 taggcagcgg caacgagccc gagactggtg agtaatccag ttacatcaag ctgtgcatgc
     2581 acaaggtctt cgtcgctgac aatcatgcgg gtcttacgcc gctggcgttc gattagccaa
     2641 acaccaccgg cgatacctgc aataagacca accgtcgcac taaagagcgc atactcaagt
     2701 cccgctggca ccccgttctg caccagtatc ggccccatgg cgacagctgt tccatgaccg
     2761 cctgcaaagc ccgcctcgag caccgaggtg aaggccagtg gctgtccaaa aaatggttta
     2821 tagacgagta tggtcgcaac gaggccaatc agcacctggc ccatcacaga gacccagatc
     2881 agcgaggttt gcgcgaagac atcggtaatt ctccgttcgg aaagacccgt attctcatgc
     2941 cgctgcagaa aaaagcaggc gaagacaagg gcgataaact ccgaaggcag ctgtttcagg
     3001 tgtaggaaaa aatccgaatt tttcgcaccg ggtatagccg tgaagagcag cagtgcgata
     3061 gcgccggctg tcagcgaagg gggtatgcgg tagcggctga accagggtat ttgttttcca
     3121 atgaagacgc tcagtccgag aaagaagagt actaaaagaa agtattgcat aacctaaagc
     3181 agaaactgcc aaatggtgcc acaggtaaag tgggatttcc tgcgtttacg cggcaggaaa
     3241 tcccgctcag cggcgggttt tggcggggcc tgccggtttt aaccagcgct tcaggaattc
     3301 gccggtcagg gaattcttct ggctcgcgat ttcttcgggt gtgccggtgg caactacctg
     3361 gccgcctttg acaccacctt caggccccat gtcaatgatg tagtcggcgc acttgataac
     3421 gtccatgttg tgctcgatga cgaccacggt attaccctca tcgacaaaac gctgcaggat
     3481 gttcatgagc agccgaatgt cttcaaagtg cagccccgtt gtcggttcgt cgagtatgta
     3541 cagtgtcttg ccggtcgacc ggcgtgatag ttctgtcgcc agcttcacgc gctgcgcttc
     3601 accgcccgag agggtagttg ccgcctggcc gagcctgacg tagccaagac cgacatcgtt
     3661 cagcgccttc agctttgtat gcaccgcggg aatagcttca aaaaaaacag cagcttcttc
     3721 aacaggcatc tcgagtactt cgtagatgtt cttgttctta aagcgtactt cgagcgtttc
     3781 ctggttatag cgtttgccgt gacagacctc gcacttgaca tacacatcgc tcaaaaagtg
     3841 catttcaatt tttttgacgc cgtctccgga gcaggcctcg cagcggccgc cttcgacgtt
     3901 gaaggaaaac cggcctgcct tgtagccgcg catctggctg gcgggcagcg cagcgaacag
     3961 gtcgcgtatc gggccaaatg ccccggtgta cgtcgccggg ttcgagcgcg gcgttcggcc
     4021 aatggggctc tggtcgatat tgataatctt gtcgatatgc tccagcccgg tgatcgactt
     4081 gtgtttaccg ggaaggtcct gggctcccat caactgccgg ttgatgccct tgaagagaat
     4141 ctcgttcacg agtgaacttt tgccgctgcc tgaaaggccc gtcacgacag tcagtaccga
     4201 tagtgggaac tcaacatcga tattcttgag gttgttttcc tgcgcaccga caatgcggat
     4261 tttctctttg cgtggttttc tgcgctcttt gggtatctcg atctgcttcc tgccggtcag
     4321 gtaatcagcg gtcagcgagt ttttggcctt cgtgagttgt gcgtaggttc cggcaaatac
     4381 tacctgtccg ccgtggcgac cggcaccggg acccatgtcg acgatgtagt ccgattcctg
     4441 cattgtctct tcgtcgtgtt cgacgacgac gatggtattg ccgaggtcgc gcagcccctt
     4501 gagtgtcgcg atcagttttg cattatcgga ctggtgcaag ccgatagagg gctcgtcgag
     4561 aacgtagagg actcccatca gcgccgagcc aatctgtgtc gcaagacgaa tacgctgtgc
     4621 ctcgcccccg gaaagcgaac cggcgatgcg gtcgagagtc aggtaaccga cgccgacaga
     4681 gttcaggaag cgcaggcggt cgaagatctc cttcatcgcc tgtttggcaa tttccatttc
     4741 agtcttgttg aactgaattt tctggaagta atcgagtgcg ccttcgagtg tcatcgctgt
     4801 cgtctcagcg atgttctttc cttttaacct gaccgcgagc gccacgggct ttaaccgttt
     4861 acccttgcat gcggggcagg gcatgtgctc catgtactgc tccatcttct ggcgcatcgt
     4921 ttctgactgc gtctgcatgt agcgccggtg caggttggga ataatacctt cgtagccgcg
     4981 cgaaaactgg taggtgctgt cgccgccttt ccactcgtag ttgagcttca ggctcttatc
     5041 gccataaagc aacaggtcga agatcttctt gggcaatttg cgccagggcg tcttcgggtc
     5101 gaatgcgact ttcttgcgca gggcatcgat cgaagccttg taccagtagg cgtcgccgct
     5161 gaaaccgagc ccgtcgccca ggccatcgcc gaccgtctgt tcgtcgtcgc gtatcagccg
     5221 gtcagggtga aactccagca tatgacccag gccactgcag t
//
LOCUS       DAOQJF010000239         5261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9129_length_5261_cov_5.211103, whole genome shotgun sequence.
ACCESSION   DAOQJF010000239 DAOQJF010000000
VERSION     DAOQJF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9129_length_5261_cov_5.211103"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLF85_12095"
     CDS             complement(<1..213)
                     /locus_tag="PLF85_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE20456.1"
                     /translation="MLSILAGILLLGICVFFHELGHFLIGKLAGVRPRVFSIGYGRGL
                     FFRRRKGTIYQITAIPLGGYVQFYGDD"
     gene            372..1814
                     /locus_tag="PLF85_12100"
     CDS             372..1814
                     /locus_tag="PLF85_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20457.1"
                     /translation="MAKKVFYNRRYKMPGWLQPYAVWAFAALITLAVVFFTSVERVGT
                     EPAQSLTAAPNGFAEGLFYELRDAEPDEKYEVTLRYAERLKKKYDLTLRETNGTGCRV
                     VNLARGSNAPWLVAAALQNGQTGGVFSVLVFLDRLAAASPSATIATALVFPRKNCNIE
                     ETVQAMQSEKTFAVFVDAENNPAPYDRINALRNLHLRRFFPSAFLMPERTHWANVLAL
                     TADAAPGRLFTVPAGRAKESVAELLAANPLLRHPAQADANAQQLSKPVAIYLGAETQL
                     HTGGFTALLVLVWLLAFIPFANALGTFRERIDLGSALTSGILYALAFVSYLLFYKLIL
                     QFAKSDFSAVVFALLLVPAVFFPLRILQKTMLRAELNRPGLHLLVQLVLTAALFTAPI
                     SGVVGLVQLTAASGFSRASLPRKLLRLLAVLAPLTLLYFAAREPMGSFANFLAAVLPA
                     FSGASLLQLIMLCIVGGNLTALLFVPREKI"
     gene            1816..2898
                     /locus_tag="PLF85_12105"
     CDS             1816..2898
                     /locus_tag="PLF85_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNE20458.1"
                     /translation="MPPRRRPATTRVAIAGCGRIAFLLEQDPLRYKPCTHLGALRYFA
                     QKDKTILPVALCDTDLTRARAAAQFLVHGRSEVLRARPVLSAVEGDGDSKTMVHGKKP
                     VLVTQSFADLLKTKPDLLVIAASTAAHFSMLTAALKARVPRIIIEKPVAFSAEEAQKL
                     RQLARKSSSVILPNYERRYHPKYIRLKQQISQSDTKASYRGFFAAGGKSLYATSDGDE
                     GVLLHDTTHLLDLAQYLFGPVKKYAVVAEKKRHVLYLEHVRGAGGLIETNLGIGVFHL
                     ELEVRLADRRITVGNGFQTTERIATSRHYRGLKSYAEPQRTGDTKFPVAKNPFVQLYR
                     EALYGAPDNAHFFEALDNVSMLTGAT"
     gene            complement(2936..4675)
                     /locus_tag="PLF85_12110"
     CDS             complement(2936..4675)
                     /locus_tag="PLF85_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNE20459.1"
                     /translation="MYKIKAICLWLAVFAATAVFAEAEKVTGKIDAVTVFTDRALVKR
                     VQAVDSADKTGVIRFTGLPQSLMSDTVRASGSGVTVTGVALRYVEKVAGDEWAEHPLK
                     KKMEKLEAQIRAEGDQISNYRDQLRTIDSMMKLTTSQSDREARLNALNVDSWEKALSF
                     LEEKRLAYQGKIRQSDEKTEKLRRDLTRVTLQFNAETQSARNSSTEVEVSFARAGQSN
                     GKIELEYMVGNVSWNGVYDLRGSAEGNEFQLVSHAIIRQSTGENWKNVAVTLSTAKPA
                     TALSPGILQPWRVSGSNFFASRPDAKRASGKLAATSAADREEVAGESPAEGADYSDSA
                     ETTTVSITLPGRENIQSDNSDHRVTLSTAPLKGTLTHVAVPSLSSYVYLKARLKNTSA
                     APVVGTMNAFLDGSFVGAITLKTPAAVGEEFDVFLGVDQRMQLKRTLKRGDVENSGLF
                     GGKVEVLNQWEIEVSNFTKKPRQVVVYDQFPVSADPGISTKFIGSSRGDMQRDANGML
                     VWKIDIKPGEKIKYDFSYSLTFPKDTWENFTRGYDAAASTKEEQYYDNLSNKPAAAPA
                     QRQYNLEKMLQKR"
     gene            complement(4687..>5261)
                     /locus_tag="PLF85_12115"
     CDS             complement(4687..>5261)
                     /locus_tag="PLF85_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20460.1"
                     /translation="GIATQWLTGAQLAQGDFHVINQDRMVQVVATPYDEEIQRLGAEY
                     NATVIPMGNAGRQEEARMQKQDAKIAASAPASGASVERALAKNTEQYSESNSWDLTTV
                     FRKKKGFANVKKDELPAELKGKSNAELEAYVKEKDAQRAKIQGRIDELNRKRNEYISR
                     QAAKSAGQSDLGTAMQTSVKEQGKKSGMAF"
BASE COUNT         1069 a         1533 c         1412 g         1247 t
ORIGIN      
        1 atcgtcgccg taaaattgca cgtaaccacc gagcggtatc gcggtaatct gatagatagt
       61 acccttgcgg cggcggaaaa acaggccacg gccgtacccg atcgaaaaaa cgcgcggcct
      121 gacgccggcg agcttaccaa tcaaaaaatg gccaagttca tggaaaaaca cacagatgcc
      181 gagcagcaag ataccggcga gaatggacaa cacggttcaa gatactaccg cagagagccg
      241 gcggaaagga atttatcatc cacccgcgat gatgccatag ctggcgtaga ggtaggttta
      301 aacctacctc tacgccagcg ccaaactatt ttgcttttcc gtgtactctg cagagcacgt
      361 ttgccaaact aatggcgaaa aaggttttct ataaccgccg ctacaagatg ccgggctggc
      421 tgcagccgta tgcagtctgg gcgtttgcgg cgctcatcac cctcgctgtt gtttttttta
      481 cctcggtgga gcgcgtgggc actgagcccg cacaatcgct gactgccgcg ccgaacggtt
      541 ttgccgaggg cctgttctac gagctgcgcg atgccgaacc tgatgaaaaa tacgaagtca
      601 cgctgcgcta tgctgagcgg ctgaaaaaga aatacgacct cacactgcgc gagaccaatg
      661 gcacaggctg ccgcgtggta aatctggctc gcggcagcaa tgcgccgtgg cttgtggcgg
      721 cggcgctgca aaatgggcaa accgggggag ttttttctgt cctggtgttt cttgaccggc
      781 tggcagcagc ctccccctct gcgactatcg cgactgcgct ggtgtttccg cgcaagaact
      841 gtaacatcga agaaacggtg caggcaatgc agagtgaaaa gacattcgcc gtgttcgtcg
      901 atgcagagaa caaccccgcg ccatacgacc gcataaacgc gctgcgcaat ctgcatttgc
      961 gccgcttttt tccgtcggct ttcctgatgc ctgagcgcac gcactgggcg aatgttctgg
     1021 cgcttaccgc tgacgccgca ccgggcaggc ttttcacggt gccggcaggc cgggcaaaag
     1081 aatcggtcgc tgaactactc gcggcaaacc cgctgttacg gcaccccgcg caagctgacg
     1141 cgaatgcaca gcagctcagt aaaccagtgg cgatctacct cggcgcggaa acccagctgc
     1201 acacgggcgg gttcaccgcg cttctggtac tcgtctggtt gctggcattc attccatttg
     1261 ccaatgcgct gggtacgttt cgcgagcgca tcgatctcgg ctcagcactt acttcgggca
     1321 ttctctacgc gctcgctttc gtgagttacc tgctctttta taagctcata ctccaattcg
     1381 ccaaatcaga tttctctgcg gtagtgtttg ccctgctgct tgtgcccgca gtattttttc
     1441 cactgcgcat tctgcagaag acaatgctgc gcgccgagct caatcggccg ggcctgcacc
     1501 tgctggtgca acttgtgcta acagccgcgc ttttcacggc gccaatcagc ggcgttgtcg
     1561 gcctggtgca gcttaccgct gcttcgggtt ttagccgcgc cagcctgcca cgcaagctac
     1621 tgcgactgct ggcggtgctg gcaccactca cgctgctgta ctttgcggcc cgcgaaccaa
     1681 tgggaagctt tgcaaatttt ctcgcggctg ttttgcctgc gttctcgggc gcgtctctgc
     1741 tgcagctgat tatgctgtgc atcgttggcg gcaacctgac agccctgctg ttcgttccgc
     1801 gcgagaaaat ctagcatgcc cccgcgcagg cggccagcga caacacgggt ggcaatcgcg
     1861 ggttgtggcc gcattgcctt cttgctcgaa caagacccgc tgcgctataa accctgcaca
     1921 caccttggcg cgcttcgcta cttcgcgcag aaagacaaga cgattctacc cgttgcactc
     1981 tgcgacactg acctgacgcg agcccgggct gcggcgcagt tcttggtaca tggacgttcc
     2041 gaggttttgc gagccaggcc tgtcctgagc gcagtcgaag gagatggcga cagcaaaacc
     2101 atggtgcatg gaaaaaagcc tgtcctcgtc acgcagagtt ttgccgattt gctaaagaca
     2161 aagcccgatc tgcttgtcat tgctgcgagc actgccgcac atttttcgat gctgacagcg
     2221 gcgctcaaag cccgggtacc gcgcatcatt atcgagaagc cagtggcatt ttcagcagag
     2281 gaagcccaaa agctgcggca attggctcga aaaagcagct cggtaattct gccaaactac
     2341 gaaagacgct accatccgaa atacatccgc ctgaagcaac agattagcca atctgataca
     2401 aaggcgtcgt atcggggatt tttcgccgca ggtggcaaga gtctctatgc gacaagtgac
     2461 ggcgacgaag gcgtgctgct gcacgacacc acccacctgc tcgacctcgc gcagtacctg
     2521 ttcggcccgg tgaaaaagta tgcggttgtt gcggagaaaa agcgtcatgt gctgtatttg
     2581 gaacacgtgc gcggcgcagg ggggctcatc gagaccaacc tgggcattgg ggtgtttcac
     2641 ctcgagctgg aggtgagatt agctgaccgg cgtatcaccg tgggtaatgg ttttcagacc
     2701 actgaaagga tcgccacgag ccggcactac cgcggtctga aatcatacgc ggaaccacaa
     2761 cgcaccggcg atacaaaatt tccggtggca aaaaaccctt ttgtgcagct ctaccgcgag
     2821 gcgctgtacg gcgcacctga caacgcgcac ttttttgagg cgttggataa tgtatcaatg
     2881 cttactggcg cgacgtaggg gtaggtttaa acctacccct gcagcgcgcc gagcactatc
     2941 tcttttggag catcttctcg agattatact gccgttgcgc aggcgctgca gcaggcttgt
     3001 tcgaaaggtt gtcgtagtat tgttcttctt tcgtcgatgc cgctgcatcg tagccgcggg
     3061 tgaagttttc ccaggtgtct ttcgggaatg tcagcgaata gctgaaatcg tatttgatct
     3121 tctcgcccgg tttgatatct atcttccaga caagcatacc gtttgcgtct ctctgcatat
     3181 cgccgcggct tgaaccgata aatttcgttg aaatacccgg atctgccgaa accggaaact
     3241 ggtcatagac cacaacctgg cgcggttttt ttgtgaagtt cgagacttcg atttcccact
     3301 ggttaagcac ttcgaccttg ccgccaaaga gaccgctgtt ctcgacgtcg ccgcgcttca
     3361 gggtgcgttt caactgcatt ctctggtcaa ccccgagaaa gacatcgaac tcctcaccta
     3421 ccgccgccgg agttttgagc gtgattgccc cgacaaaact accgtcgaga aaggcattca
     3481 tcgtgccgac aaccggggct gcgctggtgt ttttcagccg tgccttaaga taaacataac
     3541 tcgagagcga gggcactgca acatgcgtca gcgtaccctt gagcggcgcg gtactgagcg
     3601 tcacgcggtg gtctgaatta tcgctctgga tattctcgcg gccgggtaga gtaatcgata
     3661 cggtggtcgt ctccgcgctg tcagaataat cggcgccttc agcggggctt tcaccggcaa
     3721 cttcttcacg gtcggccgct gaagtcgcgg ctagttttcc cgaagcgcgc ttcgcgtcgg
     3781 gccgtgatgc aaaaaagttg gagccgctga cgcgccacgg ctgcaatata ccgggagaaa
     3841 gcgctgtcgc gggcttcgcc gtcgagagtg ttaccgccac atttttccag ttctcgccag
     3901 tactctggcg aatgatcgcg tgcgagacca gctgaaattc attgccttcg gcagaaccac
     3961 gtaggtcgta gacgccattc caggaaacat taccgaccat gtactcgagt tcgatcttgc
     4021 cattcgattg ccccgcgcgg gcaaaagaga cctcaacctc agtcgacgag ttgcgtgcag
     4081 actgcgtctc ggcattgaac tgcagcgtta cgcgcgtcag gtcgcggcgc agtttctctg
     4141 tcttttcgtc gctctgccga attttgccct ggtatgcgag ccgcttctct tcgagaaagc
     4201 tgagcgcttt ttcccaggag tcgacattca gtgcattgag gcgcgcctcg cggtcgctct
     4261 ggctcgtggt gagtttcatc atcgaatcga tggtgcgcag ctggtcgcgg tagttactga
     4321 tctggtcacc ctcggcgcgt atctgcgcct caagtttttc catcttcttc ttcaacggat
     4381 gctctgccca ttcgtcgccc gccacctttt cgacgtaacg cagcgccacc ccggttaccg
     4441 tgacacccga gcctgaggcc cgcacggtat cgctcatcag cgattggggt aaaccggtaa
     4501 aacggatcac accggtttta tcggcgctat cgactgcctg tactcttttg acgagcgcgc
     4561 ggtcggtaaa gacggtcact gcatcaattt taccggtgac cttctcagcc tctgcaaaca
     4621 ccgctgttgc ggcaaatacc gccagccaga gacaaattgc cttaatttta tacattgttt
     4681 tgctcctcaa aatgccattc ccgatttttt accctgctct ttcactgatg tctgcatcgc
     4741 agtgcccaga tcactctgcc cagcactttt tgccgcctgc cggctaatat actcgttgcg
     4801 cttgcggttc agctcatcga tgcgcccctg aatttttgca cgctgcgcat ctttttcctt
     4861 tacgtatgct tcaagttcag cgtttgattt acccttgagt tctgccggca gttcatcttt
     4921 cttcacattc gcgaatccct tcttcttccg aaagaccgtg gtgagatccc agctattgct
     4981 ttcagaatat tgttcagtat ttttcgccag cgcgcgctcg accgaggcac cgctcgccgg
     5041 tgccgaggcg gcaattttcg catcttgttt ttgcatcctg gcttcttctt gcctacccgc
     5101 attgcccatg ggaattacgg ttgcattgta ttcggcgccg aggcgctgta tttcttcgtc
     5161 gtacggcgtt gccacgacct gaaccatacg gtcctggttg atgacgtgaa aatcgccctg
     5221 cgcaagttgc gctcccgtaa gccactgcgt agcgatgcct t
//
LOCUS       DAOQJF010000240         5242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9176_length_5242_cov_4.936572, whole genome shotgun sequence.
ACCESSION   DAOQJF010000240 DAOQJF010000000
VERSION     DAOQJF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9176_length_5242_cov_4.936572"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1080)
                     /locus_tag="PLF85_12120"
     CDS             complement(199..1080)
                     /locus_tag="PLF85_12120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE20461.1"
                     /translation="MRLWLVVLLDLVLFGAFIDAQNPALEWPVEKDKKYQISSTFGES
                     RLDHFHNGIDLPGEGFKVLSPRDARLLYRINAEAIPGEMPFGGGNTIVLDHGDTWTGY
                     MHLKSVPENIVAEAPIARAEKIGISGNTGHSGGPHLHFFVYQPAERAMLNPLLIMGDT
                     YYRDTKPPLAKEWGILLIDKFASINPAKPFRMTADYPVYLLLQDQGIGRERWGVFEYR
                     VLLDDKETLNVRFDKIFFKEGIWRLSGGQPFEDIFYRNFYSLTPQVRRAKKVSVEAKD
                     LKGNSFAQTYELKIQQN"
     gene            1178..1951
                     /locus_tag="PLF85_12125"
     CDS             1178..1951
                     /locus_tag="PLF85_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HNE20462.1"
                     /translation="MNVNAYSLAESIDLAAVARRLGRNPIAQTNREMFFSEGRGFFGV
                     YSFGAVVFCNLNPADEVRLREYATGAAQNQHTQILAEDHTIKTGRLAFVPSEITVPKT
                     DAATLRVIMQNLVYSVSLDHYRRVAAELLAVVRGQSENLERKGKITLKERELHHFIGR
                     VLNIRNRMTEHFHIFGNPPEAWENKALTAVQQAMVASLEMPSRIAEVESSFAIIEENL
                     HMLNELHVHRQSHRIEIIITLLILIEVLDLIGDKLKIFH"
     gene            complement(1945..2727)
                     /locus_tag="PLF85_12130"
     CDS             complement(1945..2727)
                     /locus_tag="PLF85_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE20463.1"
                     /translation="MREASRSRTRSEKPTQKRRQTDTGLISVVIPALNEERSIALVIS
                     DLKVTARKANLQLYIIVCDNGSTDQTAAVARKAGALVVQETERGYGAACLKALAHLPA
                     ETEIVLFIDADYSDFPGEFPLLVRPIADGQADLVIGSRTAAGTVRERGSLKIQQRFGN
                     WLSTFLIRLLYGQRYTDLGPFRAISVRALQLLGMRDRNFGWTVEMQIKAARLQLRSKE
                     VPVSYRKRIGVSKISGTIKGTIMAGIIILGTIFREYFTKRDQ"
     gene            complement(2724..3317)
                     /gene="recR"
                     /locus_tag="PLF85_12135"
     CDS             complement(2724..3317)
                     /gene="recR"
                     /locus_tag="PLF85_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNE20464.1"
                     /translation="MPPELLAAIEAIQSLPGIGRRSAMRIAFDFIRQGKPYAENFNHV
                     ISQLVESIAGCDRCGAYKSQGEPCPYCDNPRRNRETLCVVEHPSDIYAVENTGEFFGY
                     YHVLGGVLSPLDGIGPNELKMPQLAERVKNLHLKEVIVAANPSVEGNATGHYIADMLK
                     PAGVRVTRIATGLALGQMLDYAETQAITQSLKQRVEL"
     gene            complement(3344..3646)
                     /locus_tag="PLF85_12140"
     CDS             complement(3344..3646)
                     /locus_tag="PLF85_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNE20465.1"
                     /translation="MGLGDIKDMMGQMRAAQQQMKQVQKKLGSMRVSAETGGGTVKAT
                     VDGEANLIDLEIDTALLDPDEIKVLPKLIKKAIQEAQKDARKQVMSDMKGMLGNMM"
     gene            complement(3693..4295)
                     /locus_tag="PLF85_12145"
     CDS             complement(3693..4295)
                     /locus_tag="PLF85_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20466.1"
                     /translation="MIKELKNVHHRLPWFGVWRIRRRINKVTARLFQKLRKDSKSLVE
                     YGNLRRGKPWGLKEGRELVWTFLVDLLADRPLIYKVVYNADEARYTCYSRSGGRNEMT
                     NVLQFTSDDIDELVARLNMLAAEIPAMRERMLRQHLYALKSEKSPKRWLDLFRKRDPA
                     EELQTLTTLREVVQSMLAQNKISKNEALKLMAYAETLHDD"
     gene            4386..4685
                     /locus_tag="PLF85_12150"
     CDS             4386..4685
                     /locus_tag="PLF85_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE20467.1"
                     /translation="MPTYDYKCADCGRIFEKFHGMNETPQVACPECSSANTARQVSLG
                     AGIVFKGAGFYVNDYKKSDASGGSATKAKSETKSESTTSTETKSAHSCGPGCAHG"
     gene            4720..>5242
                     /locus_tag="PLF85_12155"
     CDS             4720..>5242
                     /locus_tag="PLF85_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-NADP reductase"
                     /protein_id="HNE20468.1"
                     /translation="MSETPVQEFSRPTVEVNTYKKANTLKAKVLENRILFRDGDENDI
                     RHIVLDAKEMNYLDGQSVGVIPPGVNEKGNPHAVRLYSIASLASRLPHSENLTLCVKR
                     AVTKDPVTGQVYRGVASNFLCDTKPGDEVTLTGPAGRKFLLPTEAEIHRPYIFIATGT
                     GIAPFRGFLQRLFK"
BASE COUNT         1135 a         1459 c         1467 g         1181 t
ORIGIN      
        1 gttgccgctc ggcgatgtgg gactaattcg tgctatagag cagaactact gccgcgggcg
       61 caatgcttcg cagcggaagc tgcgggagct gcagcaacgc tggcaaccgt actcaaccat
      121 tgccgtctgg tacctctggc gcagcctcga tccgtatccg gtggaatatt agtaggctcc
      181 tgaaaaagga gccattagtc agttctgctg tatcttcaat tcgtacgtct gcgcgaagct
      241 attgcccttg aggtctttcg cctcgacact gacttttttc gcccggcgca cctgcggggt
      301 gagtgaatag aaattccggt aaaaaatatc ttcaaaaggt tgcccaccgg aaagccgcca
      361 gataccttcc ttgaagaata tcttgtcgaa gcggacattc agtgtttcct tgtcatcgag
      421 taagacacgg tactcaaaaa ctccccagcg ctcgcgccca atgccctggt cctgcagcaa
      481 gaggtacaca ggataatcgg cggtcatacg gaacggtttg gccggattaa tactggcaaa
      541 tttgtcgatc agcaggatgc cccattcttt cgccagcggt ggtttggtgt cgcggtaata
      601 cgtgtctccc atgatcagca gcgggttgag catggcacgc tcggccggct ggtagacaaa
      661 aaaatgcagg tgaggcccgc ctgagtgtcc cgtattaccc gagatgccaa ttttctcggc
      721 gcgcgcgatg ggagcttcag ccacgatatt ttccggcacc gatttcaggt gcatgtagcc
      781 ggtccacgta tcgccgtggt cgagcacgat ggtgttgcca ccaccaaagg gcatttcgcc
      841 tggtatagct tcggcgttga tacggtagag cagccgcgca tcgcgcgggc tgagaacctt
      901 gaagccttca ccgggcaggt cgatgccgtt gtgaaagtgg tcgaggcgcg attcgccgaa
      961 ggtggagctg atctggtatt tcttatcttt ttcgaccggc cattcgagcg ccgggttctg
     1021 cgcgtcgatg aacgcaccga acaggaccag gtccagtaat accaccagcc ataaccgcat
     1081 ggcgtctgat gcttgtcaga aatacactcc gtcaactcaa agcacaagcg tctgctgccg
     1141 cgcttgacac aacaaaaagc cggggcaggc aggtccgatg aacgtcaacg catactcact
     1201 ggccgagtcg atcgacctcg ccgctgtggc gcgccgcctg gggcgcaacc caatcgcgca
     1261 gaccaaccgc gaaatgtttt tctctgaagg ccgtggcttt tttggcgtct acagcttcgg
     1321 ggccgtcgtg ttctgcaacc tgaatccggc agatgaagtc aggttgcgtg aatatgctac
     1381 gggagcagcc caaaaccagc atacacaaat attggcagaa gatcatacaa tcaagacggg
     1441 tcggttggct tttgtcccgt cagagataac tgttcctaag accgatgccg cgacgctgcg
     1501 cgtcatcatg cagaacctgg tctattcggt ttcactcgac cactaccgcc gggttgcggc
     1561 ggagctgctg gcagtggtgc ggggccagtc agaaaatctc gagcgcaagg ggaaaatcac
     1621 gctcaaagaa cgtgagctgc accacttcat cggccgggta ctcaatatcc gtaaccggat
     1681 gaccgagcac tttcatatct tcggcaatcc ccccgaggcg tgggagaaca aggcactgac
     1741 tgcagtgcag caggcgatgg tggcaagcct cgaaatgccg tcgcgtatcg ccgaagtcga
     1801 atccagtttt gccattatcg aagaaaacct gcacatgctc aacgagttac acgtgcaccg
     1861 gcaaagccac cgcatcgaga tcatcattac tcttctgata ctgattgaag tgctcgacct
     1921 gatcggcgac aagctcaaga tttttcactg atcgcgttta gtaaaatact cgcgaaaaat
     1981 ggtgcccagg atgataatgc ctgccatgat cgtacccttg attgtcccgg aaatttttga
     2041 aacaccgata cgcttgcggt agctcactgg cacttctttg ctgcgcagtt gcagacgggc
     2101 tgccttgatc tgcatttcga cagtccagcc aaaattgcgg tcgcgcatgc cgagcagttg
     2161 cagggcacgg acgctaatcg cccggaaggg gccgagatcg gtatagcgct ggccgtacag
     2221 aagccgaatg agaaatgtcg aaagccagtt accgaagcgc tgctgtattt tcagcgagcc
     2281 acgttcgcgc actgtgccgg cagctgtgcg cgagccgatc accaggtcgg cctggccatc
     2341 ggcgatcggg cgaaccagca gtggaaactc gcctgggaaa tcggaatagt ctgcatcgat
     2401 aaagaggaca atttccgtct cggccggaag gtgcgccagt gccttgaggc aggccgctcc
     2461 gtacccgcgc tctgtttctt gtacgacgag agctccggcc ttgcgtgcca cggccgctgt
     2521 ttgatcggtt gagccattat cgcagacgat gatatataac tgcaggtttg ccttgcgtgc
     2581 cgtaaccttg aggtcagaga tgaccagcgc gattgagcgc tcttcattga gcgccgggat
     2641 aaccaccgaa atcagccccg tgtcagtttg gcgtcgtttc tgtgttggct tttctgaccg
     2701 ggtacgtgac cggcttgctt ctctcatagt tcaacgcgct gtttcagcga ctgggtaatt
     2761 gcctgcgtct cggcgtagtc gagcatctgg cccagcgcca ggccggtcgc gatacgggtg
     2821 acgcgcaccc ctgcaggttt gagcatgtcg gcaatatagt gaccggtcgc gttaccttca
     2881 acgctcggat tggccgcgac gatcacttct ttgagatgga gatttttgac ccgctcggcc
     2941 agctgtggca ttttgagttc attgggacca atgccgtcga ggggcgagag tacaccgccg
     3001 agcacatggt agtagccaaa gaactcgccc gtattttcca ccgcatagat gtctgaggga
     3061 tgttcaacca cgcagagtgt ttcgcggtta cgtcgcggat tatcacagta cgggcagggc
     3121 tcaccctggc tcttgtaagc tccgcagcga tcacagccgg cgatactttc aaccaactgc
     3181 gaaatcacat ggttgaagtt ttctgcatac ggtttgccct gtcgaatgaa gtcgaaggca
     3241 atgcgcattg ccgagcgccg cccgatgccg ggcagcgact ggattgcctc gatggcggct
     3301 aataactcgg gtggcatatt tcaatatgat gtgcggcaat gcctcacatc atattgccta
     3361 acatgccttt catgtcagac atcacctgct tgcgggcgtc tttctgcgcc tcctggatag
     3421 cttttttgat cagctttggc aggaccttaa tttcatcggg atcgagcagc gcggtgtcaa
     3481 tttcgagatc gatcaggttg gcttcaccat cgacagtcgc cttgaccgta ccgccgcctg
     3541 tttcagcaga gacccgcatg ctgccaagtt tcttctgcac ctgtttcatc tgttgctggg
     3601 ctgcacgcat ctggcccatc atgtctttaa tatcacctaa acccatggtt gcgaatgccg
     3661 aaagcagata ctgcgtaaac ggtaattagg cgttagtcgt cgtgcagggt ttcggcgtag
     3721 gccatcagct tcagggcctc gttctttgaa atcttgtttt gcgccagcat ggactgtaca
     3781 acctcgcgca gcgtggtcag cgtctgcagt tcttcagccg ggtcgcgctt gcgaaacaga
     3841 tccagccagc gcttggggga tttttcgctc ttcagagcat agaggtgctg cctgagcata
     3901 cgctccctca ttgcgggaat ttcggcagcc agcatattga gccgtgccac cagttcgtcg
     3961 atgtcgtcgc tcgtgaactg caggacattg gtcatctcgt tgcggccacc cgagcgtgaa
     4021 tagcaggtgt agcgggcctc gtcggcattg tagacgacct tgtaaatgag ggggcgatcg
     4081 gccagcagat cgacgaggaa agtccagacc agttcacggc cctctttgag cccccagggt
     4141 ttaccacgcc tcaggttgcc gtattcgacc agcgacttgg agtctttgcg cagcttctgg
     4201 aagagccggg cagtcacctt gttgatgcgg cgccggatgc gccagacccc gaaccagggt
     4261 aggcggtggt ggacattttt cagctctttg atcatgagcg cgggtgcaag ttgaggtctg
     4321 acagcggccg tcaagtagac cgtaggatcg cttgacgatc tgtttttttg ctatatattg
     4381 atcgtatgcc aacgtatgat tataagtgcg ccgactgtgg ccgtattttt gagaaattcc
     4441 acggcatgaa tgaaaccccc caggtggctt gccctgaatg cagctcggca aacacggcac
     4501 gccaggtgag cctgggcgcc ggcattgtct ttaaaggtgc gggtttctat gtgaatgact
     4561 acaagaagtc tgatgcatcg ggcgggtcag caaccaaggc caaatccgag acaaaatcgg
     4621 aatcgactac cagcactgaa accaagtctg ctcactcctg tgggccgggc tgtgcccacg
     4681 gctgaaaagc atccccagta tctctcccaa ggcggaataa tgtcagaaac cccagttcag
     4741 gaattttcac ggcccacagt cgaggtgaac acctacaaga aggccaatac cctcaaggcc
     4801 aaagttcttg aaaaccgcat cctgttccgc gatggcgatg aaaacgacat ccggcacatc
     4861 gtgctcgatg ccaaagagat gaactacctc gatggccaga gcgtcggggt gattcccccg
     4921 ggtgtcaacg aaaagggtaa cccgcacgcg gtgcgactct attccatcgc ctcgctggca
     4981 tcgcgcctgc cacacagcga aaacctgacg ctttgtgtga agcgcgctgt cacgaaagac
     5041 ccggtgacag gccaggtcta ccggggcgtc gcttcgaatt ttctctgcga tacaaaaccc
     5101 ggcgacgaag taacgctgac cgggccggcg ggccgtaaat tcctgctgcc gaccgaggcg
     5161 gagatccacc gtccctatat ctttatcgcg accggcaccg gcatcgcccc gttccgcgga
     5221 tttctgcagc gcctgttcaa ac
//
LOCUS       DAOQJF010000241         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9215_length_5228_cov_5.381983, whole genome shotgun sequence.
ACCESSION   DAOQJF010000241 DAOQJF010000000
VERSION     DAOQJF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9215_length_5228_cov_5.381983"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3195
                     /locus_tag="PLF85_12160"
     CDS             <1..3195
                     /locus_tag="PLF85_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002154852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNE20469.1"
                     /translation="GIAISSYQGGRNEYFKYTVDLLKQAGASHIKVFGGGGGVIVDSE
                     IKDLMAYGVERIYSPDDGRTLGLDGMARDMIERASKTTHPPVTPGLNPGLQDLYANYG
                     HLLSRRISLIEAQGTATPAASQSPVPVLGFTGSGGAGKSSLIDEFLRRFKLLYPELRL
                     ALLSVDPTQRKTGGALLGDRLRLNSVNDGKIFMRSLATRTDNAAISPTLKEILTYLRS
                     EKFDLIIIESAGIGQSDTAITDLCDFSVYVMTPEYGAATQLEKIAMLDYANMVIINKS
                     DRVRAEDALIQVRKQYRRNHALLTQKDEELPIYPTIANRFGDVLVDDAFAALVRHLQQ
                     KKFLPDNIAPAVKLESASVKDNFIPARRTRYLSEIADSVRSYHSAAAEQADLARKLQS
                     LYTALVTIGYEPERPLGEALEIPASQPAEPLVTQYNALLKEIDLQLLVDLRSIGKTRA
                     NYAHETYSYKVRDKEVTVRAGTKSLSDTLIAKVVPAPYEDWGELTKFLLNENIPGEFP
                     YTAGVFPFKREGEDPTRMFAGEGSPERTNRRFHLIAKGQPAARLSTAFDSITLYGENP
                     AERPDIFGKIGNAGVSICTVDDMKKLYSGFDLSAPTTSVSMTINGPSPTILAFFMNAA
                     IDQQVEAYLTREGKLAAAQEKIRQLYAEKNLKAPDYRDGKPDSHNGLGLALLGVTGEK
                     VVAPEVYENIRKATLSQVRGTVQADILKEDQAQNTCIFSTEFSLKVMGDIQQFFIDNQ
                     VTNFYSVSVSGYHIAEAGANPITQVAFTLANGFTLAEYYLSRGMKIDDFARNFSFFFS
                     NGMDPEYAVIGRVARRIWAVSMRNLYKADARSQKLKYHIQTSGRSLHAQEISFNDIRT
                     TLQALYAIMDNCNSLHTNAYDEAVTTPTPESVRRAIAIQLIINRELGLTKNENPLQGS
                     YFLTRLTDLVEEAILQEFDRISDRGGVLGAMETMYQRNKIQEESLYYEKLKTSGELPI
                     IGVNTFLSEETEYYHGELMRSTDEEKQRQIESLAQFHAFHAAESTPALDKLRKVALRS
                     GNVFEELMQAVKSCSLGQVTGALYEVGGRYRRNM"
     gene            3204..4538
                     /locus_tag="PLF85_12165"
     CDS             3204..4538
                     /locus_tag="PLF85_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20470.1"
                     /translation="MDERVTVEYVYEYWDCAACGRRGVRGDARYCPSCGKARDKNVRF
                     YRQGDKEETITEKAEQDRLSAGPDWICAFCSTTNNFHDQFCGSCGSRSDTKKSQPDRV
                     GEHPVRIKPLPAADWTCLRCGAQNKQTDEYCEGCGADYGLPPLERPAHLKQTPLTEKR
                     IDFAVYAIRGLLILLATGVLWCGYRTVSCVSNHYGTHTVVYKVTERKWKSTIVLESKN
                     LETLTAWANELPSDAKVLRREKKIRSYRDIQTGTRTESYEEEETAETTRTQEKTSVER
                     VKTGEREECATTYESIGSGAARKVTRCRKVPVYADKTVTTSTTVPATEKRRVTKEREV
                     PVYDKQPVYDTEVTYRAWRWSEVRRWQSEGTELPVQPPRPVLSPAERTANHRLSAPRT
                     VFEATFARSDKGDAPASTVQLLEEAEFAKIQPEQKVEFLFIPYKNTLERKAK"
     gene            complement(4678..>5228)
                     /locus_tag="PLF85_12170"
     CDS             complement(4678..>5228)
                     /locus_tag="PLF85_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20471.1"
                     /translation="YTSVKELSLNGAKANFKRILFEDEKLDSDNDEQYEHGMELFKKY
                     LNENWVLEYGKKSRERKLYVDIFFDFNRDRLILEVMNNRPISPKEDKRIREKFHTAMR
                     YDDIAQFYMEGGDSSEGAGMGIVLVTMMLKAQDIDPHLFTIRSDYREKTLARVEFPLS
                     SDYQPNRQRFITESNAELVLSV"
BASE COUNT         1283 a         1466 c         1421 g         1058 t
ORIGIN      
        1 ggcattgcga tcagctcgta ccagggtggg cgcaacgaat atttcaagta cacggttgat
       61 cttctcaaac aggcaggcgc atcgcatatc aaggtattcg gcggcggcgg cggagtgatc
      121 gttgacagcg aaatcaaaga cctgatggca tacggcgtcg aacgtatcta ttcgcccgac
      181 gacgggcgca cactcggtct tgatggcatg gcgcgcgata tgattgagcg tgcatcaaaa
      241 acgacgcacc cgcctgtaac cccgggttta aacccggggc tacaggatct ctacgcgaat
      301 tatggacatt tgctgtcccg gcgaatctca ttgatcgagg cacaggggac tgcgacgcct
      361 gcggcgtcgc agtcccctgt gccagtttta ggtttcaccg gttcgggcgg agcaggcaag
      421 tcttcgctga tcgacgaatt tctgcgcagg ttcaaattgc tctatcctga actacggctg
      481 gcgctgctct cggttgaccc gacgcagcgg aagaccggcg gtgcgctctt gggtgaccgg
      541 ctgcggctca actcggtcaa cgatggcaag atcttcatgc gctcgctcgc gacgcgcacc
      601 gacaatgcgg cgatttcgcc gacactcaaa gagatactca cctacttacg tagtgaaaag
      661 tttgacctga tcattatcga atctgcaggt attggccagt cagacacggc aattaccgac
      721 ctgtgcgact tttccgtcta tgttatgaca cctgagtacg gcgcggcgac gcagctcgaa
      781 aagatcgcta tgctcgacta cgcgaacatg gtgatcatca acaagtctga ccgtgtgcgc
      841 gcagaagatg ccctgataca ggtgcgtaag cagtaccgca gaaaccacgc gctactgacc
      901 caaaaagacg aagaattgcc catctacccg accatcgcca accgcttcgg cgacgtactg
      961 gtcgatgatg catttgcggc gctggtgcgc cacttgcagc agaagaagtt cttaccagat
     1021 aacatcgccc ccgcggtgaa acttgaatcg gcttcggtca aagacaattt tattcccgcg
     1081 cggcgcacgc gttacctgtc tgaaattgcc gactcggtgc gctcatacca ctctgctgca
     1141 gccgaacagg ccgatctcgc gcgcaagctg cagtcgctct acactgcact cgtcacgatc
     1201 gggtacgaac cggaaagacc actgggcgaa gccctcgaaa ttccggcctc acaacctgcg
     1261 gagccgctcg tcacacaata caatgccctg ctgaaagaaa ttgatctcca gctgctggta
     1321 gacctgcgct cgatcggtaa aacccgcgcg aactacgcgc atgagaccta cagctacaag
     1381 gtacgtgaca aagaagtcac cgtgcgcgcg ggcaccaaat cgctgagcga cacgcttatc
     1441 gcgaaagtgg tgccggcgcc gtatgaagac tggggtgagc tcacgaaatt cttactgaac
     1501 gaaaatatcc caggggagtt tccttatact gctggtgtgt tcccattcaa gcgcgagggc
     1561 gaagacccga cgcgcatgtt tgccggcgaa ggttcgcccg agcgtacgaa ccggcgcttt
     1621 cacctgattg ccaaaggtca gcccgccgcc cgcctttcca ctgcattcga ctcgatcacg
     1681 ctctacggcg aaaaccccgc cgagcgcccc gatatcttcg gcaaaatcgg caatgcgggt
     1741 gtttcgatct gcaccgtcga tgacatgaag aaactgtact cgggctttga tctttcagcg
     1801 ccgacaacga gtgtctctat gacgatcaat ggcccgtcgc cgacgattct ggcgttcttc
     1861 atgaacgccg cgatcgacca gcaggttgaa gcgtatctca cccgcgaagg aaaacttgcc
     1921 gcagcacagg agaaaatccg ccagctgtac gcagaaaaaa acctgaaagc gcctgattac
     1981 cgtgacggca aacctgatag ccataacggc ctggggctgg ctttgctcgg cgtaacgggt
     2041 gaaaaggttg ttgcgcctga agtttatgag aatatccgca aggcgacgct ctcgcaggtg
     2101 cgcggtacgg tacaggccga cattctgaaa gaagaccagg cgcagaacac ctgcatcttt
     2161 tcgaccgaat tctcgctcaa ggtgatgggc gacatccagc agttcttcat cgataaccag
     2221 gtcacgaatt tctactcggt gagtgtttct ggttaccaca ttgccgaagc gggcgctaac
     2281 ccgatcacac aggtcgcgtt cacgctagcc aacggcttca cgctcgccga gtactacctc
     2341 agccgcggca tgaagatcga cgacttcgcg cgtaacttca gcttcttctt ctcaaacggt
     2401 atggatcccg aatacgcggt aattggccgt gttgcccgcc gcatctgggc ggtttcgatg
     2461 cgcaatcttt ataaggccga tgcgcgctcg cagaaactca agtaccacat acagacgtcg
     2521 ggacgcagcc tgcacgcgca ggaaatttcg ttcaacgaca ttcgtacaac gctgcaggcg
     2581 ctctatgcaa tcatggacaa ctgcaatagc ctgcacacga atgcttacga tgaagcagtc
     2641 acgacgccga cacctgaatc ggtacggcgc gcgattgcga tacagcttat catcaaccgc
     2701 gagctgggtc tcacgaaaaa tgaaaatccg ctgcagggga gttatttctt aacgcggctc
     2761 accgacctcg ttgaggaagc gatcctccag gaatttgacc gcatctctga ccgcggcggc
     2821 gtactcgggg cgatggagac catgtaccag cgcaacaaga tacaggaaga aagcctgtac
     2881 tacgaaaagc tcaaaacctc gggtgagctg cccatcattg gcgtgaacac cttcttgagc
     2941 gaagaaacag aatattacca cggtgaactg atgcgctcga ccgacgaaga aaaacagcgg
     3001 cagatcgaat cgctcgcgca gttccatgct tttcatgcgg ctgaaagtac acctgcgctc
     3061 gacaaactac gcaaggtggc gctgcgctcg ggcaacgtgt ttgaagaact gatgcaggca
     3121 gtgaagagct gctcgctcgg ccaggtcacc ggcgcgctgt acgaagtcgg tggccgttac
     3181 aggcggaata tgtagtttgt cccatggacg aacgcgtaac cgtcgaatac gtctacgaat
     3241 actgggactg cgccgcctgc ggacgccggg gcgtccgggg cgatgcccgc tactgcccgt
     3301 catgcggcaa ggcccgcgac aagaacgtcc gcttctaccg ccagggcgat aaagaagaaa
     3361 ccatcaccga aaaggcagaa caggatcgcc tttcggccgg gccagactgg atctgtgcct
     3421 tctgctcgac gaccaacaac ttccatgacc agttctgcgg atcgtgcggg tcacgttccg
     3481 atacgaagaa atctcaacct gatcgggtgg gcgagcatcc ggtacgaatc aaaccgctgc
     3541 ctgcagccga ctggacctgc ctgcgctgtg gcgctcagaa caaacagacc gacgaatact
     3601 gcgaaggctg cggcgctgat tatggtttgc caccactgga aaggccggcc caccttaaac
     3661 aaacaccgct tacagagaag cgcatcgact ttgcggtcta tgcaatacgg ggattgctga
     3721 tactgctggc gaccggagtc ctgtggtgcg gctatcggac agtctcttgc gtttcgaatc
     3781 actatgggac gcacaccgtt gtctacaagg tcacggagcg caaatggaaa agcaccattg
     3841 tactggagag caagaacctc gaaacgctga ctgcctgggc aaacgaattg ccgtctgatg
     3901 cgaaagtcct gcgccgtgaa aagaaaatcc gcagttaccg cgacattcag accggcacac
     3961 gcaccgaaag ctacgaagaa gaagagacag ccgaaaccac gcgcactcag gagaaaactt
     4021 ccgttgaacg cgtgaagacc ggcgaacgcg aagaatgcgc cactacctat gaatcgatag
     4081 gctccggcgc ggcacggaag gtcacgcgct gcaggaaagt gccggtctac gctgataaga
     4141 ccgtgacgac gagcacaacg gttcccgcga cagagaaacg aagagtaacg aaagaacgcg
     4201 aagtaccggt ctatgacaaa caaccggtct atgataccga agtcacctac cgtgcctggc
     4261 gctggtcaga agtgcgcagg tggcaatcgg aaggcaccga gctgcctgtg cagccaccac
     4321 gacctgtact cagcccggct gaacgcacgg ccaaccacag actatcagcg ccgcgcactg
     4381 tctttgaagc aacatttgca cgcagcgaca agggcgatgc ccccgcttcg actgtgcagc
     4441 ttctggaaga agcagaattt gcgaagatac aaccggagca gaaggtggaa tttcttttca
     4501 ttccgtacaa gaataccctg gagcgaaagg ctaagtgatt ctgtgatcat tcagctatgg
     4561 gcttaagccc atagcaaaat cagccaaatg aacttgattt ccgggcgcta ctaagattcc
     4621 atcgctcagg tttggcaatt tgtaattctt gctacgggct taagcccgta gcaagaatta
     4681 cacactcagt acaagttctg cgtttgattc ggtgatgaaa cgctgccggt taggctggta
     4741 gtcagatgaa agcggaaact ccacccgagc gagcgtcttt tcgcggtagt cagagcgaat
     4801 ggtaaaaagg tgtggatcga tatcctgtgc cttgagcatc atggtgacga gaacaatgcc
     4861 catgcctgca ccttcagaac tgtcgccgcc ttccatgtag aactgcgcga tgtcgtcgta
     4921 gcgcattgca gtgtgaaact tctcgcggat gcgcttgtct tctttggggc tgatcggacg
     4981 gttgttcatc acctcaagaa tgaggcgatc gcggttaaaa tcaaagaata tatcgacgta
     5041 gagtttgcgt tcgcgtgact tcttgccata ctcgagtacc cagttttcgt tgaggtactt
     5101 cttgaagagc tccatgccat gctcgtactg ttcatcgttg tcgctgtcga gcttctcgtc
     5161 ttcaaaaagg atacgcttga aatttgcctt agctccgttc agcgaaagct ctttaaccga
     5221 ggtgtaga
//
LOCUS       DAOQJF010000242         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9216_length_5228_cov_5.298086, whole genome shotgun sequence.
ACCESSION   DAOQJF010000242 DAOQJF010000000
VERSION     DAOQJF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9216_length_5228_cov_5.298086"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /gene="hflX"
                     /locus_tag="PLF85_12175"
     CDS             complement(<1..863)
                     /gene="hflX"
                     /locus_tag="PLF85_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNE20472.1"
                     /translation="MDASSLYQKKLVSKEQYRALCVGQYGSSYFPNKRSQEESLRELL
                     MLAETAGVKTLHELFFHHTDIHPAMFFTSGQIEKIRQVLSDLQLNLLFVDAQLKPNQL
                     RNLEEELKVRVLGRIEIILDIFAMRARTREALLQVELAQLLYILPRLKGLGGVLSRQG
                     ATGAPGIGTRGPGETMLETDRRYIRRRIQKIRRELKDVERHRGTARHGRNLTTFALVG
                     YTNAGKSSVLNALSSTSQKVFAEDKLFATLDTFSRKIYLGEKDFKPNYGIVTDTVGFI
                     RNLPASLVAAF"
     gene            complement(870..1511)
                     /locus_tag="PLF85_12180"
     CDS             complement(870..1511)
                     /locus_tag="PLF85_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNE20473.1"
                     /translation="MISLRNRLQQLSGSKPVLFNTLRYGTLGVLLLSGALVAQRPDSW
                     EKERANAEAPTTKRSAEPAGNLGKPLDEVWRIVFHNSKGKDIVLDCEVARTEKEKERG
                     LMFRKFLGKNRGMIFVYNQPDQMNFWMQNTLIPLSIAYVHEKLFIASIHDMKPLSLDI
                     VSSDIPVLYAIEANVGWFKNNHILSGNRITIYKNPADYKVETKSKYRQEVPTP"
     gene            complement(1468..3102)
                     /gene="guaA"
                     /locus_tag="PLF85_12185"
     CDS             complement(1468..3102)
                     /gene="guaA"
                     /locus_tag="PLF85_12185"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNE20474.1"
                     /translation="MKAKAKQRPAAKKKAVAAKAPVKKTSTKESYHDAQRIVVLDFGS
                     QYTQLIARRLRELRYYAEIHPHTLIAEEIKKLNPAGVILSGGPSSVYEKGAPQVDKKV
                     FDLGVPVLGICYGLQLICHLNGGKVAASKEREYGRATLRARKSSPLLTGIKSGQVWMS
                     HGDKIVSLPKRFSVIGTSENSPMAAFADEKARIYGLQFHPEVHHSTQGKAILKNFASA
                     ICGMKPNWTMSRFVEEKIAEIRAQVGKERVLLGLSGGVDSTVTAKLLHDAIGDQLTCV
                     YVDTGLMRKGETEAVRQRFKKEFGIKLEVVDAEERFLKALAGVDEPEQKRKIIGKLFL
                     DEFGAKAKELGHHEFLAQGTLYTDVIESVSVKGPSQTIKSHHNRVQGVLDLIKAGRVI
                     EPLSELFKDEVRRLGQALKINRELLFRHPFPGPGLAIRILTAVTKENLATLRDADAIM
                     LDELKTSGWYQKVWQAGAIFLPVRSVGVMGDNRTYENVIALRLVTSEDGMTADFAHLP
                     YDVLAKIASRIINEVRGVNRVVYDISSKPPATIEWE"
     gene            complement(3099..3545)
                     /locus_tag="PLF85_12190"
     CDS             complement(3099..3545)
                     /locus_tag="PLF85_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20475.1"
                     /translation="MKSALVVLALALPSLVSAEEIFLSCQVTQIAASEKEGRTDAKLK
                     KIAKFLEKDAATQKYQSFRYVGKRSLNATKTSSGTAKLKNGDKLSVRVISVHRAQKKN
                     TLTIDLTVGGSSSQKSFIDRDYLIVPAGSLDKKSDLVVALHCPVFP"
     gene            complement(3599..4546)
                     /locus_tag="PLF85_12195"
     CDS             complement(3599..4546)
                     /locus_tag="PLF85_12195"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNE20476.1"
                     /translation="MRNSLRAILAGSAIKAAVLVLVATSFTACSIKKIAVRQATGFFG
                     ESRKVFEQETDLELAEASIASNLKLLEAMRVHDPKNEELNLLIAEIYSVYTLSFVEDK
                     MEQAEADGKDTEVARQRERAKQFYLRARDFAGQVLLPRLDVASMANLSEKELKDKLAK
                     LDKDDIAPLFWYAFSWGSAINLDREDVASLSELPKIEIMMAQAKAWDESYYFGGAWLF
                     EGVYFGARNEMMGGNPERSKAAFERNLKLTEGKMLITPYFYARTYCIFNQDKKCFDEN
                     LKRVLDAPGDIHPGQRLANVLAKKKAERLVKRRADFFVD"
     gene            complement(4626..>5228)
                     /locus_tag="PLF85_12200"
     CDS             complement(4626..>5228)
                     /locus_tag="PLF85_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20477.1"
                     /translation="ENLTLCVKRAIARDEATGQVYRGVASNYLCDSKPGDEILLTGPA
                     GRKFALPVAEDIHRPYIFIATGTGIAPFRGFLQRLFKHTPNFQSPVFLLFGVKTRAEL
                     LYNEEFITYTQPNFLYLKALSREMKNAAGGRYYVNDLIHEHAAKLWPAISDERAIIYM
                     CGLKGMEEGVSKAINALAAANGKGENYYEGIHHRIVEEVY"
BASE COUNT         1117 a         1455 c         1405 g         1251 t
ORIGIN      
        1 tggaaggcgg cgacgagcga agcgggcagg ttacggataa aacccacggt atccgtcacg
       61 atcccatagt tcggcttgaa gtctttctcg ccgagataga tcttgcgcga aaaggtatcg
      121 agtgtcgcga agagtttatc ttcggcaaaa accttttgcg aggtagacga gagcgcattg
      181 agcacgctgc tcttgccggc gttcgtgtaa ccgacgagcg caaaggtcgt caggttccgc
      241 ccgtgccgtg cagtgccgcg gtggcgctcg acatccttca gctcgcggcg gattttctgg
      301 atgcgccggc gtatataacg ccggtccgtt tccagcatgg tctcgccggg gccacgcgtg
      361 ccaataccgg gtgcacctgt agcgccctgc cgcgagagca ctccacccaa acccttgagg
      421 cggggaagta tatagagcag ctgggctaac tccacctgca gcaatgcttc gcgcgtgcgt
      481 gcccgcatgg caaaaatatc gagaatgatc tcaatgcggc caagcacgcg cactttgagc
      541 tcttcttcaa gatttctcag ctggttaggc ttgagctgcg catcaacaaa aagtaaattg
      601 agctgcagat cagaaagcac ctggcggatc ttttctatct gccccgaggt gaagaacatt
      661 gccgggtgta tgtcggtatg atgaaaaaac aattcatgca gggtttttac ccccgcggtc
      721 tctgccagca tcagcagctc gcgcagcgat tcttcctggc tgcgcttgtt cgggaaataa
      781 ctcgaaccgt actgccccac gcacagggcg cggtactgtt ctttcgatac tagctttttt
      841 tggtaaaggg aggaggcgtc cactgagtct cagggtgtcg ggacttcctg gcggtacttg
      901 ctcttcgtct ccaccttgta atccgccggg ttcttataga tcgtgatacg attgcctgag
      961 agaatgtggt tattcttaaa ccagccgaca ttagcttcga tggcatacaa gacaggaata
     1021 tcagagctca caatatcgag cgacaatggt ttcatgtcgt ggatgctcgc gataaaaagt
     1081 ttctcgtgta cgtaggcaat gctcaacggt atcagcgtat tctgcatcca gaagttcatc
     1141 tggtcgggct ggttgtagac aaaaatcatt ccccggttct taccgagaaa cttgcgaaac
     1201 atgaggcccc gctctttttc tttttcagtt cgcgcaactt cacagtcgag cacgatgtct
     1261 ttgcccttgg aattatgaaa cacgattcgc cagacttcgt cgagcggctt accgaggttg
     1321 cccgctggct ccgcgctgcg ttttgttgtg ggagcttcag cgttggcccg ttctttttcc
     1381 cagctgtcgg gtcgctgcgc caccagcgcg cccgacaaca gtagcacgcc taacgtgcca
     1441 tagcgcagtg tattgaacag aacgggttta ctcccactca attgttgcag gcggtttcga
     1501 agagatatca tagacgacgc ggttcacacc ccgcacttcg ttaatgatgc gggaagcaat
     1561 ttttgccagc acatcgtacg gcaggtgtgc gaagtcagca gtcatgccat cttcggaggt
     1621 cacgaggcgc aacgcgataa cgttctcata cgtgcggtta tcgcccatca cgccgaccga
     1681 gcgcaccggc aaaaagatcg ctcccgcctg ccagaccttc tggtaccagc ccgaagtttt
     1741 gagctcgtca agcatgatcg cgtcggcgtc gcgcagcgtc gccaggttct ccttggtcac
     1801 agccgtgaga atacgaatag ccagccccgg ccccgggaaa ggatggcgga acagcagttc
     1861 gcggttgatc ttgagcgcct ggcccagccg gcggacttcg tctttgaaga gttctgaaag
     1921 cggttcgatc accctgcccg ccttgatcag gtcaagtacg ccctgcacgc ggttgtggtg
     1981 tgacttgatg gtctgtgatg gtcctttgac ggacacagat tcaatgacat cggtataaag
     2041 cgtaccctgc gcgaggaatt catggtggcc aagttctttg gcttttgcgc cgaattcatc
     2101 gaggaaaagt ttgccgatga ttttgcgttt ctgttcgggc tcgtcgaccc cggcaagcgc
     2161 cttgagaaag cgctcttcgg catcgaccac ttcgagctta atgccaaatt cttttttgaa
     2221 acgctggcga accgcttcgg tttcaccctt gcgcatgaga ccggtatcaa cgtacacgca
     2281 ggtgagctgg tcaccgatcg cgtcatgcaa aagtttggcc gtgacggtcg agtcgacacc
     2341 gcccgaaagc cccaggagga cgcgctcttt accgacctgc gcgcgaattt cggcaatctt
     2401 ctcttcgacg aagcggctca tcgtccagtt gggcttcatg ccgcagatcg ccgaagcaaa
     2461 attcttcagg atcgccttgc cctgcgtgga atggtgcact tcaggatgga actgcagccc
     2521 gtagatacgc gccttctcat cggcaaaagc agccatcggc gaattctcag aagtgccgat
     2581 cacggaaaaa cgtttcggca ggctgacaat cttgtcaccg tgcgacatcc acacctgacc
     2641 tgacttgatg ccggtcagca gaggtgaact tttgcgcgcg cgcaaggtcg cacggccata
     2701 ttcacgttct tttgacgcag cgaccttgcc gccattcagg tggcagatga gctgcaggcc
     2761 atagcagatg ccgagcaccg gcacaccgag atcaaaaact tttttatcta cctgcggcgc
     2821 ccctttctca tagacagacg acggcccgcc cgagagtatg acgcccgcgg ggttgagctt
     2881 tttaatttct tctgcaatta gcgtgtgcgg gtggatctct gcatagtaac gcagctcgcg
     2941 caaccggcgc gcaatcagct gcgtgtactg gctgccaaaa tcgagcacga cgatgcgctg
     3001 cgcatcatgg tacgattctt tagtagaagt ttttttcacc ggtgctttgg ccgcgacggc
     3061 tttctttttt gccgctggcc gctgctttgc ttttgctttc atggaaacac cgggcagtgc
     3121 agtgccacca cgagatccga ttttttgtcg agcgaaccgg ccggaacaat caggtaatcg
     3181 cggtcaataa aactcttttg cgagctgctg ccgccgacag ttaggtctat ggtaagggta
     3241 ttcttcttct gcgcccggtg caccgaaatt acgcgcaccg agagtttgtc cccatttttc
     3301 agtttggccg tgcctgaact ggtttttgtg gcgttcagcg agcgtttgcc gacataacga
     3361 aagctttggt acttctgcgt cgctgcatct ttctcaagaa atttcgcaat cttcttgagc
     3421 ttcgcatcgg tacgcccctc tttttcagaa gctgcgatct gggtgacctg gcacgagaga
     3481 aaaatctctt cggctgaaac gagagaaggc agcgccaggg ccagtacgac aagggcggat
     3541 ttcatgcgac ataattaaaa tgccgcaaca ggccataaat cccttttgca ggaggctgct
     3601 agtcgacgaa aaaatcggcc cggcgtttga ccaggcgttc ggccttcttc ttggccagca
     3661 cattggccag ccgctgcccc ggatgtatgt cacccggtgc atcgagtacc cgcttgaggt
     3721 tttcgtcaaa gcacttcttg tcctggttga agatacaata cgtacgcgcg taaaaatacg
     3781 gggtgatgag catcttccct tccgtcagct taaggttgcg ctcgaatgcc gctttggacc
     3841 gttcgggatt gccgcccatc atctcgttgc gggcgccgaa atagacacct tcaaaaagcc
     3901 aggcaccgcc aaagtagtac gactcgtccc aggccttggc ttgcgccatc atgatttcaa
     3961 ttttggggag ctctgaaaga gacgcaacat cttcgcggtc caggttaatc gccgaccccc
     4021 acgaaaaagc ataccagaaa agaggagcaa tatcgtcttt gtcgagtttc gccagcttgt
     4081 ccttgagctc cttctcactg aggttagcca tactcgctac atcgaggcgg ggcaacagga
     4141 cctggccggc aaaatcgcgc gcacgcaggt agaactgctt tgcccgctcg cgctggcgcg
     4201 caacttccgt atctttacca tcggcctcgg cctgctccat cttgtcttcg acaaatgaca
     4261 gcgtatagac tgaatatatc tcagcgatca gcaggttgag ctcttcgttc ttcgggtcat
     4321 gaacccgcat cgcctcgagc agtttgaggt tagaagcgat cgaagcttct gccagctcga
     4381 ggtcggtttc ctgctcgaac accttgcggc tctcgccaaa gaaacccgtg gcctggcgca
     4441 cggctatctt cttgatcgaa catgcagtga agctggtggc gaccagaacg agaaccgccg
     4501 cctttattgc gctacccgca agtatagccc gaagagaatt tcgcataatc cactgcccca
     4561 tacctgaaac atgagggcaa gcgcagttga gaaggcagct ggcccccccc ttcccgttcc
     4621 ggctgttagt atacctcttc gactatgcgg tggtgaatgc cttcgtaata gttttcgcct
     4681 ttgccattgg ctgcagcgag tgcgtttatc gcttttgaga caccttcttc catacctttg
     4741 agtccgcaca tatagataat ggcacgctca tcgctgatcg ccggccacag tttcgcggca
     4801 tgttcgtgaa tcaggtcgtt gacgtagtag cgtccacccg ctgcattctt catctcgcgg
     4861 ctcagcgcct tcagatacag aaagttgggt tgcgtatacg taatgaactc ctcgttatac
     4921 aagagctctg cccgggtttt gacgccaaaa agcaggaata cgggcgactg gaaattgggc
     4981 gtgtgtttga agagccgctg caaaaagccc cgaaagggtg ctatgcccgt acccgtcgcg
     5041 ataaagatat agggtcggtg aatatcttcg gctacgggca gtgcaaattt tctccccgct
     5101 gggccggtga gcagaatctc atcacccggt tttgaatcgc acaggtagtt ggatgcaacc
     5161 ccccggtata cctggcccgt ggcctcatcg cgggcaatgg cgcgcttaac gcagagcgta
     5221 aggttttc
//
LOCUS       DAOQJF010000243         5187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9343_length_5187_cov_4.735191, whole genome shotgun sequence.
ACCESSION   DAOQJF010000243 DAOQJF010000000
VERSION     DAOQJF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9343_length_5187_cov_4.735191"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLF85_12205"
     CDS             <1..459
                     /locus_tag="PLF85_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE20478.1"
                     /translation="PRLSDPGSRILKLCFEHNVRVIPVPGPSALTALVSVTPFSVDPL
                     LFLGFLSPKSGRRQNELKKYLDFEGAIALFESVHRIEKTLADIALVFGDGEILVGREI
                     TKTHEEFHLGRISAAAGWAKGKQGEFALIFKADKNSRPVDTGADDASQSS"
     gene            491..754
                     /gene="fliQ"
                     /locus_tag="PLF85_12210"
     CDS             491..754
                     /gene="fliQ"
                     /locus_tag="PLF85_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNE20479.1"
                     /translation="MTDVEVISLLRDAIWLTIKLSAPALVVSLVVGLVISIIQTTTSI
                     QEQTLTFVPKLLAVLVVTLIFASWMIHSMTDYTRELMRIIGKH"
     gene            756..1544
                     /gene="fliR"
                     /locus_tag="PLF85_12215"
     CDS             756..1544
                     /gene="fliR"
                     /locus_tag="PLF85_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNE20480.1"
                     /translation="MEFLTGQFQLFTLVFARFLAMFSVAPGYSGDSLPFFSRFALAFI
                     SAALITPVAETPESLNADLQSAYFLIVLEQAAIGIFIGIVMQFLFAAYQLAGEFFSVQ
                     MGFGISEVLDPLSQVSLPVIGTLKNLIALFVFFVSGAHTYLVQALAFSVERVPHLGFS
                     FLKQGVAQNHLFKYFALLSGGMFISALKIALPIMGCLLLVSVTLGIMSKAAPQMNMLV
                     IGFSAKIVIGFMVLVILAPLLVQTMFAEMDRSLSLLDALLKGWR"
     gene            complement(1665..2411)
                     /locus_tag="PLF85_12220"
     CDS             complement(1665..2411)
                     /locus_tag="PLF85_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20481.1"
                     /translation="MQKLTAILVCLIFSGVQFLSAQEERAKAVAPSAAEKAETDKNEK
                     DEKKKGKKKAAIKLAEDREDGPPPEKDPATYELSNRRLVEDYTTLDKAPYDRGEHMFV
                     SALFGGIGGAVVGGLVGFSQYNKNDTTASQNALYLYGGAGAGVGAIIGIATTFFEQGR
                     IEQFAIGKFLMKYSWYGAVGGGLLGAGVGFIPYSSSNDYSDIFRYGGYGAGIGFVAGL
                     VMFAFDLPEHLKLYTWRREDQNVIAMSLRF"
     gene            complement(2413..3180)
                     /locus_tag="PLF85_12225"
     CDS             complement(2413..3180)
                     /locus_tag="PLF85_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNE20482.1"
                     /translation="MWRAFIPNAFSMGNLTFGFFAMIIASRATGHNTENFYYFIAAGL
                     CIMIAAVFDGLDGPLARLFRSQSQLGEQLDSLADLTTFGLAPGFLFYKMYLYEMVIAK
                     ATLGTPWDIPVGMAVAAIFPLCAAFRLARFNVAHDSGSFVGLPSPVAGLLIAFIPLYN
                     NNTQYISKPVALTIFIAVALLMISNVRYSKPQSKLRTEFTVLRAVGFALIVGLLIYRF
                     NLYFVVFGVVILYVASGLFAMVLHLLQKLRYKIVSGE"
     gene            3298..>5187
                     /locus_tag="PLF85_12230"
     CDS             3298..>5187
                     /locus_tag="PLF85_12230"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNE20483.1"
                     /translation="MLLEELGKLEKEQLLRVAEVWAVPKPPADKRALVQLLVKTFLDP
                     YHLKTVLEKLTPLQVKIYAVILNNKQVLTLGEISRKIQLQPINVEKELAVLKHIMLLY
                     QKKNRERITNNLDKYIAFDEFRKLVSTESNSRGEKFQISIRREVEQTDPENFDPKYLA
                     ILGGKGKKPREYAEKAYKDETLEKTLKTLSDGEMVLLDEAFTNGGIIEINAARIIMDE
                     QKLPMEKTIRRLDSFQLLKDIYFIDERFVRILVIPVELFNYLKKNPLFPVRSDVKELT
                     ERTIENGFDFLLNLKKLLLFISNKGLTLSQSEKLRQADMKRSEAALIDIDINLFSEKS
                     QMHQIEIILPLLRLFELVDLREENVVLIENYEEFLKREPSELLRDLFNRILEAADKRM
                     VGDEVFLPLDLPFFKMPMIETCMKLIENNEGMYVKVLLAELIRSRVIMIPGFKVSNFK
                     NAYIEQRSMIVSAMIYMYLMGMLRVDYPKRFVYISKLGRHFFHNEALEAESVQGAVIL
                     NADGTLVAIPEKMSLHDLHLLKSFADLKEFDNVYNFQLTKESVQNGVMLGNTIESFKS
                     LLVRTTRSGIPQALDFNLTEWSRDLPIVIIEESIVLLETADPRLTEALLGQIRGKKIV
                     KKEISD"
BASE COUNT         1210 a         1478 c         1340 g         1159 t
ORIGIN      
        1 ccccggcttt ctgaccccgg ctcgcgcatc ctgaagctct gttttgaaca caacgtgcgc
       61 gtaattcccg tgccgggccc ctctgcgctc acggcgctgg tgagcgtcac accgttttct
      121 gtcgacccat tgcttttcct ggggtttctt tcgcccaaat cgggccggcg gcagaacgag
      181 ctgaagaagt acctcgactt cgagggagcg attgcgctgt tcgagtcagt tcaccggatc
      241 gaaaagacgc tcgccgatat cgcgctggtt tttggcgacg gtgaaatact cgtggggcgt
      301 gagatcacga agacgcacga agagtttcac ctcggccgca tcagcgccgc ggccgggtgg
      361 gcgaagggaa aacagggcga attcgcactg atcttcaagg ccgacaagaa ctcccgcccg
      421 gtcgatactg gcgccgacga cgcgtcccaa tcttcttgac agcctgatat tattatgtct
      481 catctggctg gtgaccgacg ttgaggtaat cagcctgcta cgcgatgcca tctggctcac
      541 gatcaaactc agcgcacccg cgctcgtggt ttcgctggtc gtcggcctcg tgatcagcat
      601 catccagacg accaccagta tccaggagca gactctgacg ttcgtgccca agctactcgc
      661 cgtgcttgtc gtcaccctga tctttgcctc gtggatgatc cacagcatga cagactacac
      721 gcgcgaactg atgcgcatca tcggcaaaca ctgacgtgga gtttctcacc ggacagttcc
      781 agctctttac cctggtcttc gcccgttttc ttgccatgtt ctctgtcgca cccggttact
      841 ccggcgactc gctgcccttc ttctcgcgct tcgccctcgc ctttatttcg gccgcactga
      901 ttacgcccgt ggctgaaact cccgaatcgc tcaatgccga cctgcagagc gcctacttcc
      961 tgattgtact cgagcaggct gcaatcggta tctttatcgg tatcgtgatg cagttcctgt
     1021 tcgcggcgta ccagctcgcg ggggagttct tctcggtaca gatgggcttc ggcatcagcg
     1081 aggtgcttga tccactgtca caggtctctt tacccgtgat cggcaccctc aagaacctga
     1141 tcgcgctctt tgtctttttt gtcagcgggg cacacactta cctggtgcag gccctcgcct
     1201 tctcggtcga gcgcgtgccg cacctgggtt tttcattcct gaaacaggga gtggcgcaga
     1261 atcacctctt taagtacttc gcgctgctct cgggcgggat gttcatctcg gcgctgaaga
     1321 tcgcgctgcc gatcatgggg tgcctgctgt tggtctcggt gacgctcggt atcatgtcga
     1381 aggccgcgcc ccagatgaac atgctcgtga tcggattttc cgcgaagatc gtgatcggct
     1441 tcatggtgct ggtgatactt gccccgctgc tggtgcagac gatgttcgcc gagatggatc
     1501 gatcgctttc gctgctggat gccctgctca agggctggcg ttagcccagt tacgccaata
     1561 cagcgccggc tgtcgcaacg aaactatcgc tgttcttcaa cagccgatta gcaggttccg
     1621 gaaaaactcc cgcaccagct tcaggctcct ttttcaggag cctgctagaa acgcagcgac
     1681 atcgcgatga cgttttggtc ttcgcgccgc caggtataga gtttcaggtg ctcgggcaga
     1741 tcgaaggcaa acatgacgag accggccaca aaaccgatac ccgcaccgta accgccgtag
     1801 cggaagatgt cgctgtagtc gttgctgctt gagtacggta tgaatcccac accggcgccg
     1861 agcagaccgc cgcccacggc gccataccat gaatacttca tgagaaattt gccgatggca
     1921 aattgttcaa ttctaccttg ctcgaaaaac gttgtcgcaa tgccaatgat tgctcccaca
     1981 ccggcgcccg cgccgccata gaggtagagg gcattctgcg atgcagtcgt gtcgttcttg
     2041 ttgtactggg aaaatccgac caggccgcct actactgccc cgccaatgcc gccaaacagc
     2101 gctgaaacga acatgtgttc gccccggtcg taaggtgcct tatccagcgt tgtatagtct
     2161 tctacgagcc ggcggttgct gagttcgtac gttgccgggt ccttttcagg gggagggcca
     2221 tcttcgcgat cttcggccag cttaattgcg gcttttttct tccccttctt tttttcgtcc
     2281 ttttcattct tatcggtttc cgctttttct gcggcggacg gtgcgactgc ttttgccctc
     2341 tcttcctgcg cagaaagaaa ctgtactccg gagaaaatca ggcacaccag aatggctgtt
     2401 agcttttgca tattattctc cggacactat cttgtagcgc agcttttgca acaggtggag
     2461 caccatggca aacaacccgg atgcaacata gagaatcact acaccaaaga ccacaaaata
     2521 cagattaaag cggtagatca gcaggccaac gatcagcgca aacccgactg cccgaagcac
     2581 cgtgaattca gtccgcagct tcgactgcgg cttgctgtac cggacattac tgatcatgag
     2641 cagcgccacc gcaatgaaga tggtgagcgc aacaggcttt gagatatact gcgtgttatt
     2701 gttgtagaga ggaataaaag ctatcagcag cccggcaacg ggcgatggca ggccaacgaa
     2761 tgaccccgaa tcgtgcgcca cattgaagcg cgccaggcga aaagctgcac agagtggaaa
     2821 gatagcggca acggccattc cgaccgggat atcccaaggc gtacccagtg tcgctttggc
     2881 gatgaccatt tcgtacaagt acatcttgta gaacaaaaaa cccggtgcca gcccgaaggt
     2941 cgtcaggtca gccaatgaat cgagctgctc gcccagctgg ctctgcgacc tgaatagcct
     3001 cgctagaggt ccgtcgaggc cgtcaaagac cgcggcgatc atgatacaaa ggccggcggc
     3061 tatgaagtaa tagaagtttt ccgtgttgtg ccccgtggcg cgggacgcga tgatcatggc
     3121 gaagaagcca aaagttaggt tacccatcga gaaggcattg ggtatgaagg cgcgccacat
     3181 tttctggaaa caaaaggtac ttgcggcacg gtctggcaag gattattttc ccgctagtgc
     3241 agtcgaaaaa gcataaacag ttttacaggc agtcttgaat accgcatttt gcccgctgtg
     3301 ctgcttgaag agctgggaaa actggaaaaa gagcagctac ttagagtagc agaagtctgg
     3361 gccgtgccga aaccgcccgc agacaaacgc gcgctggtac agctgctggt caagacattc
     3421 ctcgacccct accacctgaa gacggtcctg gaaaaactta cccctctgca ggtaaaaatc
     3481 tacgctgtta ttctgaacaa caagcaggtg cttacgcttg gcgaaattag ccgcaaaatt
     3541 cagctgcaac ccattaacgt agaaaaggaa ctcgcggtat tgaagcacat catgctgctc
     3601 taccagaaaa agaaccgcga gcgcattacc aacaacctcg ataagtatat tgcctttgat
     3661 gaatttcgca agctggtatc gaccgaaagc aactcgcgcg gcgaaaagtt ccagatcagt
     3721 attcgccgcg aagtcgagca gacagacccc gaaaattttg atccgaagta cctggcgatc
     3781 ctgggtggca aaggcaaaaa accccgggaa tacgccgaaa aggcttataa agacgaaaca
     3841 ctcgaaaaaa ccctgaaaac cctgagcgat ggtgaaatgg tgctgctcga cgaggcgttc
     3901 accaatgggg gtatcatcga gatcaacgca gcgcgcatca tcatggacga gcagaagctg
     3961 ccgatggaaa aaactatccg ccgcctcgac tccttccaac tgcttaaaga catctacttt
     4021 atcgatgaac gttttgtacg catcctggtt atcccggtgg agctgttcaa ctacctcaag
     4081 aagaacccgc tcttcccggt acgcagcgat gtcaaagaac tcaccgagcg caccatcgaa
     4141 aacggttttg actttctgct gaacctcaag aagctgctgc tttttatctc taacaagggc
     4201 cttacgcttt cgcagtcaga aaagctgcgc caggccgaca tgaaacgctc agaggcagcc
     4261 ctgatcgaca tcgacatcaa cctcttctca gagaagagcc agatgcacca gattgaaatt
     4321 atactgccgc tcttgcgcct gttcgagctc gtcgacctgc gcgaagaaaa cgtcgtccta
     4381 attgaaaatt acgaagaatt tctcaagcgt gagccttcag agcttttgcg cgacctcttc
     4441 aaccgcatcc tggaagccgc ggacaaacgt atggtcggcg acgaggtttt cctgccgctc
     4501 gacctgccct tcttcaagat gccgatgatc gaaacgtgca tgaagctgat cgaaaacaac
     4561 gagggcatgt acgttaaggt actgctcgca gagctgatcc gctcgcgcgt gatcatgatt
     4621 cccggcttca aggtcagtaa cttcaagaac gcctacatcg agcagcgctc gatgattgtt
     4681 tcggcgatga tctacatgta cctcatgggt atgctgcgcg tcgattaccc gaagcgtttt
     4741 gtctacatca gcaagctggg ccgccacttt tttcacaacg aagccctcga agccgaaagc
     4801 gtacagggcg ccgttattct caacgccgat ggcaccctcg tggctattcc agagaaaatg
     4861 tcgctgcacg acctgcacct gctcaagtca tttgccgatc tcaaggaatt tgataacgtc
     4921 tacaacttcc agctgacgaa agaatcggta cagaacggcg ttatgctcgg caacacgatc
     4981 gagagtttca agtctctgct ggtccgcaca acgcgcagcg gcataccgca ggcgctcgac
     5041 ttcaatctga cagagtggag ccgcgacctg ccgattgtta tcatcgaaga aagtattgtc
     5101 ctgctcgaaa cggcagaccc ccgcctcacc gaagcactgc tcggccagat tcgcggcaag
     5161 aagatagtca aaaaagaaat ttccgat
//
LOCUS       DAOQJF010000244         5156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9431_length_5156_cov_5.530680, whole genome shotgun sequence.
ACCESSION   DAOQJF010000244 DAOQJF010000000
VERSION     DAOQJF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9431_length_5156_cov_5.530680"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PLF85_12235"
     CDS             complement(<1..1015)
                     /locus_tag="PLF85_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE20484.1"
                     /translation="MSDSQKKTQSLSLSEIRTELDRTAAELGIDVQAIGLSFERNDFN
                     LRCALPEVTDALLVDTIAIAKRHLENWRIHTFEKSFLSIQALNENLFSTDGLLDKIKI
                     RIAGIYGEKALEITKKGALLPAELSLTTAVLKRALGRAAAKDLLSELSDMGCHIYRPS
                     DSIPGIAAFTRLREKLRETVVLPLKNPDVYDRIARETRSEFSLNRPRAVLFTGPPGTG
                     KTTMARHVGKEAGLSVVHVPLENILSAYYGESTKRLGAIFDAATAAREPLILFLDEID
                     ALAPSRNEKLFEASRRLLSVLLRKIDGLETQNNIITVGATNRPQDLDSALLSRFDTIL
                     EFNE"
     gene            complement(1067..3355)
                     /locus_tag="PLF85_12240"
     CDS             complement(1067..3355)
                     /locus_tag="PLF85_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE20485.1"
                     /translation="MNGIYWHNKLARVLAGLTVLSSTVFLLFRHALIARPADTITYIV
                     AGYAFLLLILLRVLTGYRALATIMIVALTAIGLYAQSMLPEPAMGPTLVLVTAITFSG
                     IYHRLLRSLPLLVIIGFGFGLMGYSVPGHPDAGKVYLPAGVLSLSNLWYNAAVAFVVI
                     GILMVAAQSYVMNALEQNILRSENLLTATRYQKRQLEDKERLLTLFTELASDYVYQVN
                     LAEPDLVPEIFAGSFERTTGYTPEKLRQKGGWLSIVHPDDRSRLERSVPRLLESESTI
                     TEYRIVSASGRTVWLRDHVRPVRDEKTGAVTRLMGAVTDISERKAAEEQIENLAFYDP
                     LTSLPNRRLLLDRLEQALALSARTGQHGALLFIDLDHFKNLNDTKGHEAGDQLLVQQA
                     RRLNLCVREGDTVARLGGDEFIIILGELSANAEQAAEETSEITQRILKALSVPVALTF
                     KTMADYENTCSIGVALFLGHQVSVDELLRRADMAMYQAKADGRNLFRFFDPQMQRLLS
                     ERLALEEDLKHALAHRQLEIFLQPQVTEGNRMVGAEVLLRWRHPERGMVAPSEFIPAA
                     ERTGLIVEIGAWVLEQACLVLKRWAEKPGTAGLVLAVNVSARQFRQPDFVSQVSTVLQ
                     MTGARGECLKLELTESLALENIEETIERMHALRALGISIALDDFGTGQSSLSYLKRLP
                     LNQLKIDQAFIRDITTDPNDAVLVQTIIGMGQNLNLDIIAEGVETEAQHSFLVRHGCP
                     AYQGYLFSPPVAVAEFEQKYAA"
     gene            complement(3425..4588)
                     /locus_tag="PLF85_12245"
     CDS             complement(3425..4588)
                     /locus_tag="PLF85_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNE20486.1"
                     /translation="MEFFYLAITLAAGTGIGFAIGRLAGKAAAIAATTENRLLREQLQ
                     KLEAEQGALAQKIHEEFKNVANQVLVSGSRQVHEDAQKQLATLLNPLQNKLGEFQNRL
                     EQTATAQGRDSAALKEQIRSLTDLNKNVGEEARRLADALKGDAKARGNWGEMVLERLL
                     EVAGLAKGTHYETQVTLKGDGAEFRPDVIVRLPDQKDMIIDSKVSLVHYEQMFAAAND
                     EARSSLRKEHLLRIKKHIDDLGKKDYSSLDGVNSIGLVFMFVPIEGAFLEALAADNLL
                     YDYAFGKNVVILTPGTLLVTLRLVAQMWQQDNQNKNALKIAVEGGKLYDKFVGFVEDI
                     QRLGSQMETTRKTYDDAVRKLNTGPGNLVTQTEKLRELGAKTKKKLALTSDDE"
     gene            complement(4631..>5156)
                     /locus_tag="PLF85_12250"
     CDS             complement(4631..>5156)
                     /locus_tag="PLF85_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20487.1"
                     /translation="RTPLEMLSAPGVAVVGNAANQVFSTHGSGVVPALQSARLLANAV
                     RGAKDPGDADALWQYTAAFQRTLGAKLAAYNLFRRFSQSLTGEEIGKMFRYGFMTEPM
                     VFAGMKQDLPAPSVGTGLSVLTTVFRDLPFAAKLVRALAAMPLVLLHYRNFPMSRDAA
                     KFAAWVERARALGV"
BASE COUNT         1097 a         1500 c         1447 g         1112 t
ORIGIN      
        1 gttcgttaaa ttccagaatg gtgtcgaagc gcgaaagcag cgcgctgtcc aggtcctgcg
       61 gccggttggt tgccccgacg gtgatgatgt tgttctgtgt ttccagtccg tcaatcttgc
      121 gcaacaacac cgagagcagg cgccgcgagg cctcgaaaag tttttcgttg cgcgaaggcg
      181 ccagcgcatc aatttcatca agaaaaagaa tcaggggttc gcgtgctgca gttgcagcat
      241 caaaaattgc accgagccgt ttcgtcgatt cgccatagta tgccgagagt atattctcca
      301 gcggtacgtg caccaccgac aacccggctt ctttaccgac gtgacgggcc atcgtggtct
      361 taccggttcc gggcgggccg gtgaacaata ccgcgcgcgg ccggttcagg gaaaattcgc
      421 tgcgcgtttc gcgggcgatg cggtcataga catccgggtt cttgaggggg agtaccacgg
      481 tttcgcgtaa tttttcgcgc agccgtgtga aggccgctat gccgggaata ctgtcgctgg
      541 gccggtagat gtggcacccc atatcggaga gttcagacag aagatctttt gcggcggcac
      601 ggccaagcgc gcgcttcagg actgcggtcg tcagcgaaag ctccgccggc aacaatgccc
      661 ccttcttcgt tatttcgagc gccttttcgc catagatgcc ggcaatacgg atcttgatct
      721 tatcgagcaa accatcggtc gaaaacaggt tctcgttcag cgcctgtatc gagagaaacg
      781 atttttcaaa agtatggatg cgccagtttt ccaggtggcg cttggcgatc gcaatggtgt
      841 cgaccaggag cgcgtcggtc acttcgggca gcgcgcagcg caggttaaaa tcgttgcgct
      901 cgaagctcag gccaatagcc tgcacgtcaa tccccagctc ggcagccgtc cggtcgagtt
      961 ctgtccgtat ttcgctgagg cttagggatt gagttttttt ctgcgaatcg ctcatagttt
     1021 gccggcagcc tgtgagaagc caacgctacg cccaacagaa aattactcag gcggcgtact
     1081 tctgctcaaa ttctgcgacg gcaaccggcg ggctaaacag gtagccctgg taagccgggc
     1141 atccatggcg cacgagaaaa ctgtgttgcg cctcggtttc gacaccttcg gcaatgatgt
     1201 ccaggttcag gttttgcccc ataccaatga ttgtttgcac cagtacagca tcattcgggt
     1261 ctgtagtgat atcgcgtatg aatgcctggt caattttcag ctggttcagc ggcaggcgct
     1321 tcaggtaaga aagcgacgac tgcccggtgc caaagtcgtc aagcgcaatg cttataccga
     1381 gcgcgcgcag ggcgtgcatt cgctcgatag tttcttcgat gttctcgagc gccaggcttt
     1441 cggtcagttc aagctttaaa cattcacccc gagcaccggt catctgcagc acggtactga
     1501 cctggcttac aaaatccggc tggcgaaatt gcctggcgct gacgttcact gccagtacca
     1561 gcccggcagt tcccggtttt tcagcccagc gcttcaatac aagacaagcc tgttcaagca
     1621 cccaggcgcc gatttcgacg atgagccccg tgcgctctgc cgcgggaata aactcagaag
     1681 gggcaaccat gccgcgctcg gggtggcgcc agcgcagaag cacctcagcg ccgaccattc
     1741 ggttaccttc tgtgacctgc ggttgcaaaa aaatctcgag ctgccggtga gccagcgcgt
     1801 gcttgaggtc ttcttcaagc gcaagacgct cggaaagcaa ccgctgcatc tgcggatcaa
     1861 aaaagcggaa caggtttcgc ccgtcggctt tcgcctggta catggccata tctgcgcggc
     1921 gcagcagctc atcgacagac acctggtgac caagaaaaag ggcaaccccg atactgcagg
     1981 tattctcgta gtcagccatg gtcttgaatg tcagggcaac cggtacgctg agcgccttga
     2041 ggatgcgctg cgttatttca gaagtctctt cggcagcttg ttctgcattt gcggataatt
     2101 caccgagtat aatgatgaat tcatcgccgc ccaggcgtgc cacagtatca ccttcgcgca
     2161 cacacaggtt caggcggcgc gcctgctgca caagcagctg gtcgccggct tcatgcccct
     2221 tcgtgtcgtt caggttcttg aaatgatcaa gatcgataaa taagagagcg ccatgctgcc
     2281 cggtacgggc actcagcgcc agggcctgtt caagccgatc gagcaacagg cgacggttgg
     2341 gcaatgaggt cagcggatcg tagaaggcga ggttttcaat ctgttcttct gcagctttac
     2401 gctccgaaat atcggtcacc gcccccatga gcctggtcac agcgccggtt ttttcgtcgc
     2461 gcacgggccg aacgtggtcg cgtaaccaga cggttcgtcc cgaagccgaa acgattctgt
     2521 attcggtaat cgttgattct gattcgagca ggcgcggaac agagcgctcg aggcgcgacc
     2581 tgtcatcagg atgcacgatg ctgagccagc cacctttctg gcgcagtttc tccggggtat
     2641 accccgtcgt ccgctcgaat gatccggcaa aaatttccgg cacgaggtca ggctcagcga
     2701 ggttaacctg ataaacatag tctgaagcca gctcagtgaa gagcgtcaac aaccgttctt
     2761 tgtcttcgag ctggcgcttc tggtagcgtg tagccgtcag caggttttca ctgcgcagga
     2821 tgttctgctc cagcgcattc ataacatacg attgcgccgc aaccatgaga ataccgatga
     2881 caacaaaagc cacggcggca ttgtaccaca ggttactcag cgagagaaca cccgccggca
     2941 ggtaaacctt acctgcatcc ggatgacctg gcacagagta gcccattaag ccaaaaccga
     3001 aacctattat gacaagcaga ggcaggctgc gcagcaggcg gtggtagatt ccggaaaatg
     3061 tgatagcggt caccagtacc agcgtcggcc ccattgcagg ttcaggcagc atggactgcg
     3121 cgtacaaacc gatcgccgtg agtgcaacga tcatgatggt cgcaagcgca cggtagccgg
     3181 tcagcacccg cagcagaata agcaggagga atgcataacc tgcaacaatg taggtaatag
     3241 tatctgcggg acgcgcgatg agagcgtgac gaaacagaag aaagactgtg ctgctgagaa
     3301 ccgtcaggcc agcgagaacg cgcgcaagct tattatgcca gtaaatgccg ttcatcacca
     3361 aaacctgaac gacagtagcc gataccggta gactgaaaag cgattaacgc gacagatcgc
     3421 cggcctattc gtcatcagaa gtcagcgcaa gtttcttctt ggttttagct cccagttcac
     3481 gcagcttctc cgtctgcgtg accaggttac ccggcccggt attcagcttt cgcacagcgt
     3541 cgtcgtaggt tttgcgtgtg gtttccatct ggctgccgag gcgctgtatg tcttcgacaa
     3601 agccgacaaa cttgtcgtac agcttcccgc cttcgaccgc aatcttgagc gcgttcttgt
     3661 tctggttatc ctgctgccac atctgcgcaa cgaggcggag cgtcaccagg agcgtgcccg
     3721 gcgtcagaat caccacattc ttaccgaacg catagtcgta gagaaggtta tctgccgcaa
     3781 gcgcctcaag aaacgcgcct tcaatcggta caaacataaa gaccaggcca atgctattca
     3841 cgccgtcgag actgctgtaa tccttcttgc cgagatcgtc gatatgtttt ttgatgcgca
     3901 gcaggtgctc tttgcgcaaa gacgagcgcg cctcatcgtt tgcggccgca aacatctgct
     3961 cgtagtgcac cagcgacacc ttcgaatcga tgatcatgtc tttctggtcg ggcaggcgca
     4021 caatcacgtc gggcctgaac tccgcaccgt cgcctttcag cgtcacctgc gtttcatagt
     4081 gcgtgccctt cgcgagaccg gcaacctcga gcaggcgctc gagcaccatc tcgccccagt
     4141 tgccgcgcgc cttggcatcg cccttgagcg cgtcggccaa acggcgtgcc tcttcgccga
     4201 cgtttttgtt gagatcggtc agcgagcgta tctgctcttt gagcgctgcg ctgtcgcgcc
     4261 cctgcgcggt cgccgtctgc tcgaggcggt tctgaaactc gcccagctta ttctgcagcg
     4321 ggttcagtaa ggtggccagc tgcttctgcg catcttcatg cacctggcgc gaccccgaga
     4381 cgagcacctg gttcgcgaca tttttgaact cttcgtggat tttctgcgcc agcgcgccct
     4441 gctcggcctc gagcttctgc agctgttcgc gtagcaggcg gttttccgtc gtggcggcta
     4501 tcgctgcggc ttttcctgca agcctgccaa tggcaaagcc gatgccggtt ccggctgcga
     4561 gtgtgattgc aagatagaaa aattccatgt ttctcctgat gccttcgcct aagacagcgg
     4621 taaataattt ttagacaccc agtgcccgcg cgcgctcaac ccacgcggca aattttgccg
     4681 catcgcggct catgggaaag ttacggtaat gcagcagcac gagcggcatc gcggctaaag
     4741 cgcgcaccag cttcgccgca aacggcagat cgcgaaatac cgtggtcaga acgctgaggc
     4801 cagtacccac ggacggcgcg ggcagatcct gcttcatgcc cgcgaacacc atgggttcgg
     4861 tcataaagcc gtagcggaac atcttgccga tttcttcgcc ggtcaggctc tgcgaaaacc
     4921 ggcgaaacag gttataggcc gcgagtttgg cgccaagcgt gcgctggaac gctgccgtgt
     4981 attgccagag cgcatcagca tcgccggggt cttttgcccc gcgaaccgca ttggccagca
     5041 gccgcgccga ctgcagcgcc ggcacaacac cactgccgtg cgtcgaaaat acctgattag
     5101 cggcattgcc cacaacggct acgccgggcg cactcagcat ctcgagcggg gttctg
//
LOCUS       DAOQJF010000245         5154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9439_length_5154_cov_4.835458, whole genome shotgun sequence.
ACCESSION   DAOQJF010000245 DAOQJF010000000
VERSION     DAOQJF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9439_length_5154_cov_4.835458"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLF85_12255"
     CDS             complement(<1..1285)
                     /locus_tag="PLF85_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20488.1"
                     /translation="MAKKLLFLPIFIALHCAGSDAMYDPPGGKGFWARKSDLTTGKTS
                     FYRVTSMFAAASAHANLYVEAKQEVPKSSIDGLLAAFENNIVPVEHVWFTTPTDVDQN
                     GKVILLLLDIQDGYRDGSSSGYIAGYFDPLNHYSDASVNALNSQYHSNQAEMLYLDTY
                     PADVTRSAFFSTLAHEYQHLLQFGKYYKGDQDDIEPTWIDEGLSEVSSDLTGFGPQVS
                     RANTFRSALINSTSLIREPAQFGLEHYASTYMYFRYLADAYGLGLISAIFNQNQVGLS
                     GLNSAIQGSDSGLLTLCRNNSGLTYPYFGCSYRYMWGTLLSGSGPGGSPTSTNVVYIG
                     VSEGTLQVGTRTYRLDVSNASFLQELTTTLSNGSYNSSGQTTGALQSYAPRLFKNNGT
                     GSSPGFSSCSGSPPCGLTAIVGSSYFVIFNHDANTS"
     gene            1436..1690
                     /locus_tag="PLF85_12260"
     CDS             1436..1690
                     /locus_tag="PLF85_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20489.1"
                     /translation="MNVRESQQQLEGTLAKSIDSAEGLHRMAAAGIFEQLGQIAPLSG
                     VVQGIRPAYENGAAQVFEMGRTLNRSAGELARTVLDRVGM"
     gene            1691..2506
                     /locus_tag="PLF85_12265"
     CDS             1691..2506
                     /locus_tag="PLF85_12265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE20490.1"
                     /translation="MAHQFGSGTYVISGASRGIGRALAVMLAQRGANLVLIARDKKAL
                     ALAQAECKKENPSGKIDTFSCDLSNRKAIETTCATIRRKHPVLAGIINNAGYARPGYF
                     HELPADEFDRAVKTDYLGAVHLTRGLHALVQKGGLISFTSSVVGYMGVFGYSSYAGPK
                     FALVGFAETLRQELDQRGVQVSVLCPPDTETPGYSEENKTKPMETQALSESAKLMTAE
                     AVAAKFIKAVEKGKFIITCNFESGMLYRLHGIAPSLVFRIMMGMIRKAQKKLK"
     gene            complement(2524..3357)
                     /gene="panC"
                     /locus_tag="PLF85_12270"
     CDS             complement(2524..3357)
                     /gene="panC"
                     /locus_tag="PLF85_12270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNE20491.1"
                     /translation="MEIIRTRAAMREYARQHSANTGFVPTMGYLHEGHLSLVRRAKAE
                     NRKVVVSIFVNPAQFNDPKDLEKYPVDFERDAAMLEGEGVDAVFAPMREEIYPDGVPA
                     LRMSYDAIMNKLCGAHRPGHFEGVLLVVHNLFMWVQPRRAYFGLKDYQQYLLISKMAH
                     DLELPVEVIGCPLVREADGLAMSSRNVRLSASGRQQALTISRALFTAAELWRSGAEVA
                     ETEAALHSALAALQVEYALVCDAKTLVTLTGKESRPKNVVIAVAAFVDGVRLIDNILL
                     K"
     gene            complement(3406..4269)
                     /locus_tag="PLF85_12275"
     CDS             complement(3406..4269)
                     /locus_tag="PLF85_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE20492.1"
                     /translation="MVFRRKKKEGSARILGYKASYLHFPAKKPAGTLVCFHGWLDNAA
                     SFVPLAEALPEYEIFAWDFLGHGKSAHKHRGERYHYVDLVPFIEAALRHVDRTEIVLV
                     GHSMGAGASALYAGAIGESLKALVTIEGLSPMTAEPADAPKILADGVREFSKALNLPK
                     PVYASPDDALKIRMRVNGLSREAAMPLVSRAIQKVARGITWRADFRLRAPSLVRMTLP
                     QVKSLLSAIQVPALLVLGDKGMAELTRIAETDADIGKIFEVVKLPGHHHLHMDHAVPV
                     AAAIREFLARS"
     gene            complement(4284..4958)
                     /locus_tag="PLF85_12280"
     CDS             complement(4284..4958)
                     /locus_tag="PLF85_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNE20493.1"
                     /translation="MIRLHGYPISNYYNRVKMALRLKGLGFEEVRAAPSKDEAYLKIN
                     PLGVIPTLEIDGVFLAETHPMLEYIEEKFPEMPRLLPADPADRHRVRQLVNYVDIHMD
                     KPIRMLRDFHTLDASTPAALKEIVLRELKQAVQSFNATARFAPYVAGNEISLADCAAY
                     CTIPWFGLLAERHLGENPLSGIQGFAEYRAFMDQNPDFAEIHKTAEKQIKVIERAKKF
                     AGKVGL"
BASE COUNT         1179 a         1451 c         1431 g         1093 t
ORIGIN      
        1 ccgaagtgtt tgcgtcatgg ttaaagatca caaaataact tgagccgacg attgccgtta
       61 aaccgcaggg cgggctgcca ctgcagctgg aaaaccccgg actggatccg gtgccgttat
      121 ttttgaatag ccgcggggca tagctctgca aagcgccagt ggtctgaccc gacgaattgt
      181 aagagccgtt tgataaagtc gtcgtgagct cctgcaagaa gctggcattt gagacatcca
      241 accggtaagt acgtgtaccg acctgaaggg taccttcact cactccgata taaacaacat
      301 tcgtgctggt aggtgaacca ccgggaccag atcccgatag cagcgttccc cacatgtacc
      361 tgtacgagca gccaaagtaa ggatatgtga gaccagagtt attccggcag agcgtgagca
      421 gaccagaatc gctcccctgt atggcgctgt taagcccact caggccaacc tggttctgat
      481 taaaaatggc agagattaaa cccagaccgt aagcatcggc aaggtaacga aaatacatat
      541 aagtgctggc gtaatgctca agaccaaact gcgccggctc gcggatgagc gatgtactgt
      601 tgatcagcgc actgcgaaag gtattcgcac gactaacctg tggaccaaac cccgtcaggt
      661 cagaagaaac ttcagaaagc ccctcgtcga tccacgtggg ctcaatgtca tcctggtcgc
      721 ccttgtaata tttaccgaac tgcagcaggt gctggtactc atgcgcgagt gtagagaaaa
      781 aggcacttcg tgtcacgtcg gcaggataag tgtccaggta gagcatctct gcctggttac
      841 tgtggtattg actattgagc gcgttaacag aagcgtcgga ataatggttg agcgggtcaa
      901 agtaacccgc gatgtagccc gaagagctgc cgtcgcggta accatcctgg atatcgagga
      961 gcagcagaat cactttgcca ttctggtcga cgtcggttgg ggtcgtgaac cagacatgct
     1021 ctaccggaac gatattgttt tcgaaagctg ccagcaagcc gtcgatggaa gacttcggca
     1081 cttcctgctt ggcttcgacg tagagattcg catgcgccga agcagcggca aacatcgagg
     1141 tgacacggta gaagcttgtc tttccagtcg tcaggtcgct cttgcgtgcc cagaaacctt
     1201 tgccaccggg tgggtcgtac atggcgtcac tgccggcaca gtgcagggca atgaagatcg
     1261 gtagaaaaag cagctttttc gccacggctg agccgcactc cattttgagc ccggcgtcaa
     1321 ggcgaaaaca ccgcatcctg ctcctttggc ccgaaaattt tggcacgcgc gcggccccgc
     1381 agcaatcgcc gtaattgtgt tggcagacca tgcgtgattt gcaagcctga ctaccatgaa
     1441 cgtgcgtgaa agccagcaac aacttgaagg tacgcttgcc aagtctatcg actctgccga
     1501 aggcctacac cgcatggcgg cagcgggcat cttcgaacaa ctaggacaga tcgcgcccct
     1561 ttcgggggtt gtgcagggca tacgcccggc atatgagaac ggcgcggcac aggtctttga
     1621 aatgggccgg acgctcaacc gttcagcggg tgaactcgcg cgcacggttc ttgaccgggt
     1681 gggaatgtaa atggcacacc agtttggttc aggcacttac gtcatcagcg gcgcgtcccg
     1741 cggtatcggc cgtgcgctgg cagtgatgct cgcgcagcgc ggtgcaaacc tcgtgctgat
     1801 cgcccgcgac aagaaggcgc tcgcgctcgc acaggccgag tgcaaaaaag aaaatccctc
     1861 tggtaagatc gacacgttca gctgtgatct ctctaaccgc aaggcgattg agaccacctg
     1921 cgcgacaatc cgcagaaaac accctgtact cgcgggaatc atcaacaatg ccggttatgc
     1981 ccggcccggc tattttcacg aactgccagc cgacgagttt gaccgggctg tgaagactga
     2041 ctatctcggc gcagtacacc tgacgcgcgg cctgcatgcg ctcgtgcaaa agggtggcct
     2101 gatcagtttt acatcctctg tcgtgggcta catgggcgtt ttcggctata gctcgtatgc
     2161 cggccccaag tttgccctgg tcggttttgc cgaaacactg cggcaggagc tagaccagcg
     2221 cggcgtacag gtcagtgtac tctgcccgcc cgataccgaa accccgggat acagcgaaga
     2281 gaacaagacc aaacccatgg aaacgcaggc actgtcagag agcgcaaaac tgatgaccgc
     2341 agaggcagtt gcagcaaaat ttatcaaagc tgtagaaaaa ggcaagttca tcatcacgtg
     2401 caactttgaa tcaggcatgc tttaccgcct gcatggtatt gcaccgtcgc tggttttcag
     2461 gatcatgatg ggtatgatcc gcaaggcgca gaaaaaatta aaatgatctg cgctccgcgc
     2521 agatcatttt aatagaatat tatcaatcag ccttacgcca tcgacaaatg ccgcaacggc
     2581 gatgaccacg tttttcggcc gggactcttt accggttaat gtgaccaggg tctttgcatc
     2641 acagacgagc gcatattcaa cctggagcgc tgccagcgca gaatggagcg ccgcttcggt
     2701 ttctgcaacc tccgccccac tccgccataa ctccgctgca gtaaaaagcg cccgcgaaat
     2761 cgtgagcgcc tgctgccggc cgctggccga aagccgcacg ttgcgcgacg acatcgcaag
     2821 accatctgcc tcgcgcacga gcgggcaacc aatgacctcg accggcagct cgagatcatg
     2881 cgccatcttg ctgatcagca ggtactgctg gtaatctttg aggccaaagt aagcgcgccg
     2941 tggctgcacc cacataaaca ggttgtgcac cacgagcagt acaccttcaa aatgacccgg
     3001 ccggtgcgca ccgcagagtt tgttcatgat cgcgtcatag ctcatgcgca gcgccggcac
     3061 gccgtcgggg taaatttctt cgcgcatggg agcgaaaacg gcatcgacac cttccccttc
     3121 aagcattgcg gcatcgcgct cgaaatccac cgggtatttt tcgagatctt tgggatcgtt
     3181 gaactgcgcc gggttcacaa agatcgaaac aacgaccttg cggttttcgg cctttgcccg
     3241 ccggacaagc gacagatgac cttcgtgcag gtagcccatc gtcggcacaa agccggtatt
     3301 cgcagaatgt tgccgcgcat attcgcgcat cgcggcacgg gttcgtatta tttccaaatt
     3361 gtctccagct taatcatgct caatgaatac acggctacag tcatgtcagg acctggctaa
     3421 gaattcgcgg atcgcagctg ccacgggtac cgcatggtcc atgtgcaggt ggtggtgacc
     3481 gggcaatttt actacctcga agattttgcc gatgtcggca tcggtttcgg caatacgcgt
     3541 gagctccgcc atacctttgt cacccagcac cagcagcgcc ggcacctgta tcgctgacag
     3601 caggctcttg acctgcggca gcgtcatgcg cacgagcgaa ggtgcgcgca ggcgaaaatc
     3661 ggcgcgccac gtgatacctc tcgcgacttt ttggatcgcg cgggaaacca gcggcatcgc
     3721 cgcttcgcgg ctcaggccat tgaccctcat cctgatcttg agcgcgtcat cgggcgatgc
     3781 atagacaggc tttggcagat ttagcgcttt agagaattcg cgcacaccgt ctgcgagaat
     3841 tttcggcgcg tccgctggtt cggcggtcat cggcgagagg ccttcaatgg tgaccagggc
     3901 tttgaggctt tcgccgattg cccccgcata gagcgccgat gcgcccgccc ccattgagtg
     3961 gccgaccagc acaatttcgg tacggtcaac atggcgcagc gcagcttcga tgaaggggac
     4021 gagatcgacg tagtggtagc gttcgccgcg gtgtttgtgc gcgctcttgc cgtgaccgag
     4081 aaaatcccac gcgaaaattt cgtactcggg cagtgcctcg gcaagcggca cgaacgacgc
     4141 agcgttgtcg agccagccat ggaaacagac gagcgtgcct gccggttttt ttgccgggaa
     4201 gtgcaggtaa cttgccttat atccgaggat gcgagccgaa ccctctttct tcttacggcg
     4261 aaagaccatt gagcgcaaat tacctagaga ccgactttgc cggcaaactt ctttgcgcgt
     4321 tcaatcacct tgatctgctt ctctgcagtc ttgtggattt cggcaaaatc gggattctgg
     4381 tccatgaacg cccggtactc ggcaaaaccc tgtatacccg acagcgggtt ttcaccaagg
     4441 tgacgctccg caagcaggcc aaaccacgga atcgtacagt acgctgcgca gtcggcaaga
     4501 gagatttcgt tgcctgcgac gtacggggca aaacgcgcgg tcgcgttaaa actttgcacc
     4561 gcctgcttca gctcacgcag cacaatttct ttcagtgcag cgggcgttga ggcgtcaagg
     4621 gtatgaaaat cacgcagcat acggatcggc ttgtccatat ggatatcgac gtagttgacc
     4681 agctgccgca cgcggtggcg gtcggcggga tcagccggca gcaggcgggg catttcagga
     4741 aatttctctt cgatgtattc gagcatcgga tgcgtctccg caagaaagac tccatcgatc
     4801 tcgagcgtag gaataacgcc gagcgggtta atcttcaggt aagcttcgtc tttcgacggc
     4861 gcagcacgta cctcttcgaa cccaagcccc ttcagccgca gcgccatttt gacgcggtta
     4921 tagtagttac tgatcgggta accgtgcagg cgtatcatcg aaggaaagaa ggatgctcga
     4981 tctgacgggt caatatttta attgtctgca catttgcctt accatcccgt gccgctatgc
     5041 tctctgaccg gttcatgcgc acgctgattc tgctctcgct ggcggtaatt ccggcaatgc
     5101 tgaattgccg tggcgccgtt caggatatgg aaatccgcca tgacatccgc tatc
//
LOCUS       DAOQJF010000246         5126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9519_length_5126_cov_5.889371, whole genome shotgun sequence.
ACCESSION   DAOQJF010000246 DAOQJF010000000
VERSION     DAOQJF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9519_length_5126_cov_5.889371"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..974)
                     /locus_tag="PLF85_12285"
     CDS             complement(174..974)
                     /locus_tag="PLF85_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNE20494.1"
                     /translation="MRAAVIQMNSQNDTGANLDAAGLLLADAARQGATYALLPENFAF
                     LGSEQEKLSKGKEISQRALDFLRQAAAQHNLTITGGGMPMPAASGKFYNAALTVTPQG
                     GILHRYDKLHLFDAMPGDNVSYRESRSTEAGEPHLETFETAGIAIGMSICYDVRFPAL
                     YRAYARKGAQALCVPAAFTRLTGAAHWHVLLRSRAIENTCYVLAAAQGGEHFGSRQTF
                     GHSMIIDPWGEIVAELNERPGVAVADLDPRRLEEVRQRLPSLRHDKIV"
     gene            1084..1371
                     /gene="rpsF"
                     /locus_tag="PLF85_12290"
     CDS             1084..1371
                     /gene="rpsF"
                     /locus_tag="PLF85_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNE20495.1"
                     /translation="MAKKNYELVLVLSDKNGDDKTRSREEAEASLTKRGAEIIQKEDW
                     GARRLYHLSKKQDRGHYFYYGFKSDGSAIQQINGDLRVNAGVLKAMITVLD"
     gene            1373..1813
                     /gene="ssb"
                     /locus_tag="PLF85_12295"
     CDS             1373..1813
                     /gene="ssb"
                     /locus_tag="PLF85_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE20496.1"
                     /translation="MASDINRVILIGRLTRDPELKSTNSGNYFCRFSLASNRNVYNKQ
                     SGESRDEVGFFDCIAWGKSAETIHKYLQKGRRICVEGSLRWSSWEGTDGKKQSKVEIN
                     VEGFQFLDAKQDGGGSVRDTGPSEPSGSAAHFDTGAMSDDDIPF"
     gene            1839..2126
                     /gene="rpsR"
                     /locus_tag="PLF85_12300"
     CDS             1839..2126
                     /gene="rpsR"
                     /locus_tag="PLF85_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNE20497.1"
                     /translation="MEERQEREPRREEGGYEERKGRGDRGPRFKKKTCRFCEVKDMAI
                     DYKRVDVLVKLVSNKGKILPRRLTGTCALHQRAVAKAIKKARNAGFMPFSV"
     gene            2140..3171
                     /locus_tag="PLF85_12305"
     CDS             2140..3171
                     /locus_tag="PLF85_12305"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HNE20498.1"
                     /translation="MEAVLGFPLSLAFALIPIYLYRPSFNRRLWLWGVFLLTGLFLLL
                     PGKLRIEAFLPGGAAPFFFIAGVWVVALNPTSSIYKLLNMPLPSRKELKEEIEKVPDV
                     EGRLQKYLALTMQLKKIWLKRDQFLSGYNSAMLLIFSIAATLVSFGVAVYEYFLTGTL
                     KQLLEEIYKQVAARMQASGAEVVDFRPQLLELSPVFFFASSFASVFCLGIFLRIFARL
                     RFKQNLVQGYMSLFRIPDNWVWGLIGFSGIYLLGLKIEALKPATFVVRNGMLILLFLY
                     LLQGIGVASLFFEVRLVPTHWIALGVMLMGLWVPELLTLIALFFTVLGLAEVWFALRK
                     RSLRPVTDA"
     gene            3181..3624
                     /gene="rplI"
                     /locus_tag="PLF85_12310"
     CDS             3181..3624
                     /gene="rplI"
                     /locus_tag="PLF85_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNE20499.1"
                     /translation="MRVILKQDIPTLGRAGDIKEVRDGYARNFLFPRGLVMNANVRSV
                     KEKAFLEQVQARKIAKRKKSAEELAGQLNGKEVTLQAKVGEDNKLFGSITNIQVAKQL
                     EAMGFTVDRRAIVLDENIKALGNYKVQIKLHDGVNAAIQLHVVAG"
     gene            complement(3621..>5126)
                     /gene="priA"
                     /locus_tag="PLF85_12315"
     CDS             complement(3621..>5126)
                     /gene="priA"
                     /locus_tag="PLF85_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNE20500.1"
                     /translation="IAQALARGEQALLLVPEIALSGQIMDVIRQRFGNRSVLYHSGLS
                     DGERFASQQAFLSGAAGIAVGTRSAVFLPAAKLGLIIIDEEHDGSFKDQRSLRFDTRV
                     VARLRQQCEPQVPRFILGSATPSLESLMRAKEGHAQLYRLRERATGLALPKVFIPDYH
                     TQQGLISPFLMEKMHEHLGDKNQVLLLMNRRGHSTHVHCPACDAFAECPRCSVTLTYH
                     KDNTLRCHHCGHSETYARFCPKDGEPRHLSGRGIQKVEDILEAQFSSYSYARLDRDTT
                     RQKNFANDVLQGMRERQIDILLGTQMIAKGFDIEGVTLVGVLSIDPVLNAPDYRASEH
                     AWQLLAQVVGRSGRHRPGEVVIQTMEPQHPAVVAAAQHNADTFYAAEAELRRLTHYPP
                     FGALARFLLRSDNEQELFTFADRLAREIRPQNTRELFGETSQESIEVLGPASPGRHKL
                     ENEFRVHFLIKAPSEAALRSYLKRVVPLCERMARLAGRISLVLDIDPRETN"
BASE COUNT         1081 a         1433 c         1482 g         1130 t
ORIGIN      
        1 ggttacccat tcggcgaccg ttgtcgactc tctgcaacct tcacccggtg gagctacaga
       61 gactgcaccg cctttacccg accctgtaac cgaaacgccg ctcgagcacc gcgcgatgca
      121 ctggcatttt ccgatgccca aagcccagct tgagctgctc agcccgtaac ctgtcagaca
      181 attttgtcgt gcctcagcga cggcagtcgc tggcgcactt cttcgaggcg tctagggtcg
      241 agatctgcca cagctacacc cgggcgttca ttcagttcgg cgacgatttc accccatggg
      301 tcaatgatca tgctgtggcc aaaggtctgc cggctaccga aatgttcacc gccctgcgca
      361 gcagccagca cgtagcaggt attttcaatc gcgcggctgc gcaacagcac gtgccagtgc
      421 gcagcgccgg tcaaccgcgt gaaggcagcc ggtacgcata acgcctgagc tcccttgcgt
      481 gcataggcac ggtagagcgc gggaaagcgc acgtcgtaac agatactcat gccgatggca
      541 atgcctgcag tttcgaaggt ttcaaggtgc ggttcaccgg cttccgtcga gcgcgattcc
      601 cggtagctga cattgtcgcc cggcatcgca tcaaacaggt gcagcttgtc gtagcggtgt
      661 agtattcctc cctggggagt caccgtcagc gccgcgttat agaatttccc gcttgccgca
      721 ggcatgggca tgcccccgcc ggtgatggtc aggttatgct gcgctgcagc ctgccgcagg
      781 aaatccagcg cacgctggct gatttctttg cccttactca gtttttcctg ttcactgccg
      841 agaaaggcga agttttcggg cagcagtgca taggtagcac cctgccgcgc ggcgtcggcc
      901 agcagcagac cggcggcatc gaggttggcc cccgtgtcat tctgcgagtt catctggatt
      961 acggctgcgc gcatgggaac aaaacccctt tttgctttcc agcctgtctg aaaagcctaa
     1021 gttgggtgcg ccttctggat cgggtcaaca gacacctttc cttaaaaaac catagggggt
     1081 tacatggcaa agaagaacta cgagctcgtt cttgtgctct cagacaagaa tggcgatgac
     1141 aaaactcgtt cgcgcgaaga agcagaagct tcactcacca agcgcggcgc tgagattatc
     1201 caaaaagaag actggggtgc aagacgattg taccacctgt ccaagaagca ggaccgcggt
     1261 cactactttt actacggttt caagagcgac ggctctgcaa tccagcagat caacggtgac
     1321 ctgcgcgtga atgcaggcgt gctcaaagca atgatcaccg tgctcgactg atatggcgag
     1381 cgacatcaac cgtgttatcc tgatcgggcg cctgacgcgc gatccggagc tcaagtcgac
     1441 caactcggga aactacttct gccgcttctc acttgcgtcc aaccgcaacg tgtataacaa
     1501 gcagtccggc gagagccgcg acgaagtcgg tttctttgat tgcatcgcct ggggcaaatc
     1561 agccgagact attcacaagt acctgcagaa gggccgccgc atttgtgtgg aaggcagcct
     1621 gcgctggtct tcgtgggaag gcaccgatgg caagaaacag agcaaggttg agatcaatgt
     1681 cgagggattc cagtttctcg acgcgaagca agacgggggc ggttcggtgc gggataccgg
     1741 cccctcagaa ccatcgggct cggctgctca ctttgacacc ggcgcgatga gcgacgacga
     1801 tatcccgttc tagttatagt tacactcaag aggaaaaaat ggaagaacgt caagaaagag
     1861 aaccacgccg cgaagaaggc gggtacgaag agcgtaaagg tcgcggcgac cgtggcccac
     1921 gcttcaagaa aaagacctgc cgtttctgcg aagtaaaaga tatggcaatt gattacaagc
     1981 gcgtggacgt gctggtaaaa ctcgtatcaa acaaaggtaa aatactgccg cgccgcctga
     2041 caggtacctg cgcactgcac cagcgtgcag tcgcgaaggc gatcaagaaa gcacgcaacg
     2101 ccggctttat gccattctcg gtataaaaca gcagaaatca tggaagcggt gctgggtttt
     2161 cctttaagcc ttgcattcgc tctcatcccg atctaccttt accgcccgtc attcaaccgc
     2221 cgcttgtggt tgtggggagt tttcctcctg acgggccttt ttttgctcct gccaggcaag
     2281 ctccgcattg aagcttttct gccgggtggg gcagctcctt tcttttttat cgccggtgtc
     2341 tgggtagtgg cactgaaccc cacgagttcg atctacaagc tgctcaatat gccactgcca
     2401 tcgcgcaaag agcttaaaga agagattgaa aaagtgccag atgtcgaagg tcgcctgcag
     2461 aagtacctgg ccctgacgat gcagctgaag aagatctggc tcaagcgcga ccagttcttg
     2521 tcaggttata actccgccat gctgctgata ttcagcatag cggcgacgct ggtatcgttc
     2581 ggtgtcgctg tctacgagta tttcctgaca gggacactga agcagctgct cgaagaaata
     2641 tacaagcagg tggcggcgcg catgcaggct tcgggtgctg aagtcgtcga ctttcggccg
     2701 cagctgcttg agctgagtcc tgtgttcttt tttgcctcaa gctttgcctc ggtgttttgc
     2761 ctcggcatct ttctgcgtat ttttgcgcgg ctgcgcttta agcagaacct tgtccagggt
     2821 tacatgagcc tgttccgtat tccggacaac tgggtctggg ggctgattgg tttctccggc
     2881 atctacctgc tcggccttaa gatcgaggcg ctgaagccgg caacttttgt tgtgcgcaac
     2941 ggtatgctga tcctgctgtt tctctacttg ctgcagggaa taggggtcgc atcgctgttt
     3001 ttcgaggtgc gtctggttcc cacgcactgg atcgcgctcg gcgtgatgct catggggctc
     3061 tgggttcccg aacttctgac cctcatagcc ctgtttttta cggttcttgg gctggctgaa
     3121 gtctggtttg ccctgcggaa aaggtcttta cgccctgtca ccgatgcctg atttgatcta
     3181 atgcgcgtca ttctcaagca agatatccct acgctcggcc gtgccggcga catcaaagaa
     3241 gttcgtgacg gttacgcccg taacttcctg tttccccgtg gcctggtaat gaacgcgaat
     3301 gtgcgttcag tcaaagaaaa ggcctttctt gagcaggtac aggcgcgcaa gatcgccaag
     3361 cgcaagaaat cggcagaaga gctggcgggc cagctgaatg gcaaagaagt taccctgcag
     3421 gcaaaggtcg gcgaagataa caagcttttt ggcagtatca ccaacatcca ggtggcaaag
     3481 cagctcgaag caatgggctt tactgttgat cgccgcgcga tcgttctcga cgagaacatc
     3541 aaggcactcg gtaactacaa agtacagatt aaactccacg acggcgtgaa cgctgctatc
     3601 cagctgcacg ttgttgctgg ttaattcgtt tctctcgggt ctatatcgag taccaggctg
     3661 atccgtccgg ccagcctggc cattctctca cacaaaggaa caacgcgctt cagatagctg
     3721 cgcagggctg cctcagaggg tgccttgatc aaaaagtgta ccctgaattc attctccagt
     3781 ttgtggcgcc ccggcgatgc ggggcccaat acttcgatgc tttcctgtga ggtttcaccg
     3841 aaaagttcgc gcgtgttctg cgggcgaatt tcacgcgcca gcctatcggc aaaggtgaac
     3901 agctcctgct cgttgtcgct gcgcagcaag aaccgcgcga gtgccccaaa gggggggtag
     3961 tgtgttaacc ggcgcagctc ggcctctgca gcgtaaaagg tgtctgcgtt gtgctgcgct
     4021 gcggcaacga ctgcagggtg ttgcggttcc atggtctgta tgacaacttc accggggcgg
     4081 tgccggcccg aacggccgac cacctgtgcc agcagctgcc aggcatgttc tgaggcgcgg
     4141 tagtcgggcg cattgagcac cgggtcaatc gacagtactc ctaccagcgt aactccttca
     4201 atatcaaaac ccttcgcgat catctgcgtc ccgagcagaa tgtctatctg tcgctcacgc
     4261 atgccctgca ggacgtcgtt ggcgaagttc ttctgccgcg tggtgtcacg gtcgaggcgg
     4321 gcatacgaat aggagctaaa ctgcgcctcg agaatgtctt caaccttttg gatgccccgg
     4381 ccggacaagt ggcgcggctc gccgtctttc gggcaaaaac gtgcataagt ttcggaatga
     4441 ccgcagtggt ggcagcgcag ggtattatct ttgtggtagg ttagcgtgac cgagcagcgc
     4501 gggcactcag caaatgcgtc gcatgccggg cagtgcacgt gcgtgctgtg cccgcgccgg
     4561 ttcatcagca gcagtacctg gttcttgtcg cccaggtgtt cgtgcatctt ttccatcagg
     4621 aacggagaga tcaggccctg ctgggtgtgg taatcgggta taaaaacctt cggcagcgcc
     4681 aggcccgtgg cgcgctcgcg cagccggtag agctgggcgt ggccttcttt ggcgcgcata
     4741 agagattcga gcgagggggt ggctgaaccg agtatgaacc gtggcacctg cggttcgcac
     4801 tgctgcctca gccgggcgac aacacgcgta tcgaagcgca acgaacgctg gtctttgaac
     4861 gagccatcgt gctcttcgtc gatgatgata aggccaagtt ttgccgcggg caaaaagacg
     4921 gcggagcgcg tgccgacagc gatgccggct gcgcccgaaa gaaaggcctg ctggctcgcg
     4981 aatctctctc cgtcgctcag gcctgaatgg tagagaaccg aacggttacc gaaccgctgc
     5041 cgtatgacat ccatgatctg gcctgacagg gcaatttcgg gtaccagcaa cagcgcctgt
     5101 tcgccgcggg cgagggcctg cgcaat
//
LOCUS       DAOQJF010000247         5083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9635_length_5083_cov_5.728719, whole genome shotgun sequence.
ACCESSION   DAOQJF010000247 DAOQJF010000000
VERSION     DAOQJF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9635_length_5083_cov_5.728719"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLF85_12320"
     CDS             complement(<1..667)
                     /locus_tag="PLF85_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNE20501.1"
                     /translation="MKPHQSALSADQAYFLEELYEKYQTQPQSMTSEWVEFFRDLERG
                     GGRAQNAELEAGSGMDVSLLKEMGVQNLLNAYRSRGHLAANLDPLGILKPNRAIIEDR
                     LNNLTREDLENEFDTEVPGLGVARLRDVVRWFEQTYCASIGSEHYYLRNAQERTWLQY
                     KMEVSANNEQLSKEQQLRIFEKLYQADYFEKFLGKKFVGKKRFSLEGGDALIPMLDAA
                     VETA"
     gene            811..2808
                     /locus_tag="PLF85_12325"
     CDS             811..2808
                     /locus_tag="PLF85_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE20502.1"
                     /translation="MSPNSDQALPKQKRVYLRILGYAARYKWRFLLGALLSLFVSVFN
                     ALSLTALKPIFDVIETGGNKPFQLHFDKDEVAYAKSGPLRKDLQVLLAKHPSPEFFDK
                     ERDYFAKVEALPADDIRGKIKYFLAEKKTNLNLFLLQYHAMKVLMFVAFLVLPIYLLK
                     LLCDLGTVYFMSSTGLRAVNNIRDDLYHKLLDLPMSFFVKEKTGVMMSRIINDANLLS
                     DSASDYLRVSINNFFIITTHLVLLSLISFKLLIITVIGVPLVLWPVNHYARKIKDITQ
                     REQTSLADLTGHMQEVISGIRIIRAFGMEKFEHAQFSHINESLFRQIFKFRMNNALSP
                     ALVEFTTSLLIIGLIVYGAARIIDGEFTSGSFFLFLFTLMVIISPIKQIASWYNMVQR
                     ATAAGERIFSLIDTPNDVSEAAHAKPFDVLRKNIRFQGVNFHYPQTEAQVLRNISFDV
                     PVGSRVALVGHSGAGKSTLVDLIPRFYDVTEGKITFDGEDVRDLRIADLRRAIGVVTQ
                     EIFLFNGTVRQNISYGRDDIAQEQIEHAAEMAYADVFIKNLPQRYDTQIGERGLMLSG
                     GQRQRLSIARAILKDPQVLILDEATSALDTESERLVQKALEKLMKNRTVFVIAHRLST
                     IYNADQILVMNKGRIVERGTHKELIRKKGHYKKLYDMQFRE"
     gene            2842..3822
                     /locus_tag="PLF85_12330"
     CDS             2842..3822
                     /locus_tag="PLF85_12330"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE20503.1"
                     /translation="MAKHAKDQKLRFELPAGERLDRFLAAALAEHGVTLSRSAIQRQI
                     EQGGVEGLPAGQTKTGFRGKRPVEITFTLPAAQDKQLVPINAGIPVLYEDEYLAIVHK
                     PPGMTVHPGAGTGNDTMVHALLGQFDKLSPHHERPGIVHRLDRETEGLMVIAKTEQAR
                     HRLSASFAERQIQKRYSALVWGSVTLPEEVSGYIWRDLKNRKKMKFGDRPPNNLARLR
                     TAELLITEQVRHKFATELEIELITGRTHQIRATLEHYHAPLIGDAVYGDDAGKCRLYK
                     IGKEKREKLAQSGMLLVARSLAFKHPFKRRNLEYAIELPERFAAARALLG"
     gene            complement(3851..>5083)
                     /locus_tag="PLF85_12335"
     CDS             complement(3851..>5083)
                     /locus_tag="PLF85_12335"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNE20504.1"
                     /translation="LHAHPYYNSEQVSQRYVPIKDDRTWYALPASLANRPEVHALHQQ
                     AFATYTGLVDALRPVVEDIFFSIHRLKARNKEDHADAIKKRCMEVARYVMPVSTVAYL
                     YHSVSALTLMRYARMLLWSGHAEGVILGVQMFELVRSVDPDLFAELPEPLVPPARSYD
                     AAAAGRSNSAFDQLLEGKPASLLSASVAALFADTACSQLNSEAASALDTARNPLLGDV
                     FYPGTLDETTRILNHAHFTFRKKLSHTADSQEQRHRTLPGNRPILASQVSLENDYITP
                     SLVAQAPEARSLYENYLAQNFDLVRALWQKDEIPREDISYLLPNAFPVRFLESGDFYN
                     FYHKWKARLCYNAQEEIFYSAHAEVSAVKKGWPELGKHIGPPCVVREHLKPRCPEGDH
                     FCGIKVWQLPFEEYERVI"
BASE COUNT         1137 a         1333 c         1430 g         1183 t
ORIGIN      
        1 ccgcagtttc tacagcggca tcgagcatcg gtatcagcgc atcgccccct tcaagagaaa
       61 aacgtttctt gccgacaaat ttcttgccaa ggaacttctc gaagtaatct gcctggtaga
      121 gtttctcgaa gatgcggagc tgctgctctt tggagagttg ttcattgttg gctgaaacct
      181 ccatcttgta ctgcagccag gtacgttcct gtgcattacg caggtaatag tgttcagagc
      241 caatgctcgc acaataggtc tgctcaaacc agcgcacgac atcacgcaac cgggcgacgc
      301 caagcccagg aacctcggta tcgaattcgt tctccaggtc ttcgcgcgtc aggttgttca
      361 gccggtcttc aataattgcc cggttgggtt tcaggatccc gagaggatcg aggtttgctg
      421 cgaggtgccc ccggctgcgg tacgcgttca gcaggttttg aactcccatt tccttgagca
      481 gggagacgtc cattccggag cccgcttcca gttcagcgtt ctgggcacga ccgccaccgc
      541 gttcgaggtc gcggaagaat tcgacccatt ccgaagtcat gctttgcggc tgggtctggt
      601 atttttcgta aagttcttcg agaaagtacg cctgatcggc agacagggca ctctggtgag
      661 gtttcatact ttcgtggcgt ggttcgctat accgtagtca agttcttttt aaatgcagct
      721 tcggacggcg cgcggagaga gactgaaaga cacgacccgt tgtgcaccaa aagcgctttt
      781 cgccctgtcg aaaacccccg attcgcaggg atgtctccga attctgacca ggctttaccg
      841 aaacagaaac gcgtctacct gagaattctg ggttatgcgg cgcgttataa atggcgcttc
      901 ttgctggggg ccctgctttc gctctttgtc tctgtgttta atgctctcag tctgacggct
      961 ctgaaaccga tttttgacgt gattgaaacc gggggcaaca agccgttcca gctacatttc
     1021 gacaaagatg aagtggcata cgccaaatcg ggccccctgc gcaaagacct gcaggtcttg
     1081 ctggcaaagc atccttcgcc ggagtttttc gacaaggagc gcgactattt cgccaaggtt
     1141 gaagctttgc ctgcagatga tatcaggggc aagataaagt acttccttgc tgaaaaaaag
     1201 acgaacctta acctgtttct gctgcagtac cacgccatga aagtgctcat gttcgtggcg
     1261 ttcctcgtgc tgccgatcta cctgctgaag ctattgtgcg acttgggtac agtatacttt
     1321 atgagctcca ccgggttgcg cgccgtaaat aatatacgcg acgaccttta ccacaagctg
     1381 ctcgatctgc cgatgtcgtt ctttgtcaag gagaagacgg gcgttatgat gagccgcatt
     1441 atcaatgacg caaacctgct ctcagattca gcttctgatt acctgcgcgt ttctataaat
     1501 aactttttta tcattacgac ccatctggtg ttattgagtt taatcagttt caagctgctg
     1561 attattaccg tgatcggagt acccctggtg ctctggccgg tgaatcacta tgcccgtaag
     1621 atcaaagata tcacgcagcg tgagcagaca agccttgccg atctcaccgg gcatatgcag
     1681 gaagttattt ccggtatccg gatcatacgc gcctttggga tggagaaatt cgagcatgcg
     1741 cagttttctc atataaacga atctctgttt cggcagatat tcaagttccg gatgaacaat
     1801 gcgctctctc ctgcccttgt tgagttcacg acgagcctgc tgatcatcgg tcttatcgtc
     1861 tatggcgctg cacgcatcat tgacggcgaa tttacttcag gaagtttctt cctcttcctg
     1921 tttaccctca tggtgatcat cagcccgatc aagcagattg cctcgtggta taacatggtg
     1981 caacgggcaa cagccgcagg cgaacgtatt ttcagtctta tagatacacc gaatgacgtg
     2041 agtgaagccg cacacgctaa acctttcgat gtgctccgca agaacatacg cttccaggga
     2101 gtgaattttc actatccgca gaccgaggcg caggttctga ggaatatttc gtttgatgtt
     2161 ccggttggtt cgagggtggc gctggtcggg cattcaggtg ccggcaagtc gacgctcgtc
     2221 gacctgattc cccggtttta cgacgtgacg gaaggcaaaa ttacctttga tggcgaagat
     2281 gtgcgcgatc tgcggattgc agacctgagg cgggcgatag gggttgtcac gcaggagatt
     2341 tttctcttca acgggacagt ccggcagaac atctcttatg gtcgcgatga tatagcccag
     2401 gagcagatcg agcacgccgc cgaaatggcg tacgctgatg tctttatcaa gaacctgccg
     2461 cagcgttatg atacgcagat cggtgaacgc ggactgatgc tttcgggtgg ccaacggcaa
     2521 cgcctgtcta ttgcccgtgc gattctcaaa gatccgcagg ttcttattct ggatgaggcg
     2581 accagcgcac tggatactga gtccgagcgc ctggtgcaaa aggcgctgga aaaactgatg
     2641 aagaaccgga cggttttcgt catcgcgcac aggctttcga cgatctacaa tgccgaccag
     2701 attcttgtca tgaacaaagg ccggattgtc gaacgcggca ctcataaaga gctgatcagg
     2761 aaaaaggggc actataagaa actctatgac atgcagtttc gcgagtagca ccggctgaga
     2821 ttaaccggta aactggatca aatggcgaag catgcaaaag accagaagtt acgctttgag
     2881 cttccagcag gcgagcgcct cgatcgcttc ctggcggctg cactggcaga acacggtgtt
     2941 actctctctc gctctgccat ccaacggcag attgagcagg gcggtgtaga aggcctgccc
     3001 gcggggcaaa ccaagaccgg ttttcgcggt aagcgaccgg tcgagatcac tttcacgctg
     3061 cccgcggcac aggataagca gctcgtaccc attaatgccg gaattccggt actctacgaa
     3121 gatgaatacc tggcaattgt gcacaagccc ccaggcatga ctgtgcaccc tggagcaggc
     3181 accggcaacg atacgatggt gcatgcgctg ctgggccagt ttgataaact ttcacctcac
     3241 catgagcggc ccggaattgt gcaccggctg gatcgtgaaa ccgaagggct catggtgatt
     3301 gccaagacag agcaggcgcg tcaccgcctg agcgcgagtt ttgccgaacg gcagatccaa
     3361 aagcgctact cggcgctggt ctggggaagc gtgacactgc cagaagaagt gagcggttat
     3421 atctggcgtg atctcaagaa caggaaaaag atgaagttcg gcgaccgtcc gccgaataac
     3481 cttgcgaggc ttcgcacggc agaactcttg ataactgagc aggtcaggca taagttcgcc
     3541 acggagctcg agatcgaatt gattaccggc cgcacacacc agatacgcgc gacgctggag
     3601 cattaccatg caccgctgat cggcgacgcg gtttacggtg atgacgctgg taagtgcagg
     3661 ctgtacaaga tcggcaaaga aaagcgtgag aaacttgcgc agtcgggcat gctgctcgtc
     3721 gcccgttcgc tggcctttaa gcatcctttc aagcggcgca atcttgagta cgcgattgag
     3781 ctgccggagc gttttgccgc agcgcgtgcg ctgctcggtt aagtaacagc ctgcgcagaa
     3841 actcccacgt ttatattacg cgttcgtatt cttcgaaggg cagctgccag actttgatgc
     3901 cgcaaaaatg atcgccttcg gggcagcgtg gtttgagatg ttccctcacg acgcagggcg
     3961 ggccgatatg tttgccgagt tccggccagc ctttcttgac cgcactgact tctgcgtgcg
     4021 ctgaatagaa gatttcttcc tgcgcgttgt agcacaggcg cgccttccac ttgtggtaga
     4081 aattgtagaa atcccccgac tcaagaaacc tgacaggaaa ggcgttaggt agcaggtaag
     4141 aaatatcttc gcgcggaatt tcgtctttct gccacaatgc gcgcacaagg tcgaagtttt
     4201 gcgccaggta gttttcatag aggctgcgtg cctcaggcgc ctgtgcaacc agcgagggag
     4261 ttatataatc attttccagt gaaacctgcg atgcgagtat cggccggtta cccggcagcg
     4321 tgcggtgccg ctgttcctgc gaatcggcag tgtgcgagag cttcttgcga aaggtaaagt
     4381 gcgcatggtt gaggatgcgc gtcgtttcgt cgagtgtacc cgggtagaac acgtcaccca
     4441 gcagcggatt gcgcgccgta tcgagtgcag atgcagcttc agagttcagc tggctgcagg
     4501 cggtgtcggc aaaaagcgcg gcaaccgagg ccgagagcag cgaggcaggt tttccctcta
     4561 gcagctggtc gaatgcagag ttactgcgcc cggcagcagc ggcatcgtag ctccgtgccg
     4621 gaggtaccag cggttcaggc agttctgcga acaggtcggg atcaaccgag cgcacgagtt
     4681 caaacatctg gacgccgagg atcaccccct ccgcatggcc cgaccagagc agcatacgcg
     4741 cgtagcgcat cagggtgagc gcgctgacgc tgtggtagag atacgctacg gtcgataccg
     4801 gcatcacgta ccgcgcgact tccatgcagc gctttttgat cgcatcggcg tggtcttctt
     4861 tgttgcgggc tttcagccga tgaatggaga agaatatatc ttcgaccacg gggcgcagcg
     4921 catcgactag gccggtatag gtggcaaagg cctgctggtg cagcgcgtgt acttccggtc
     4981 tgtttgcaag actcgcgggc agggcatacc acgtcctgtc gtctttgatg ggcacataac
     5041 gctgcgacac ctgctcagaa ttgtagtagg ggtgcgcgtg cag
//
LOCUS       DAOQJF010000248         5070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9673_length_5070_cov_5.960917, whole genome shotgun sequence.
ACCESSION   DAOQJF010000248 DAOQJF010000000
VERSION     DAOQJF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9673_length_5070_cov_5.960917"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLF85_12340"
     CDS             complement(<1..240)
                     /locus_tag="PLF85_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803228.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNE20505.1"
                     /translation="MKPVRFVTATSLFDGHDASINIIRRFLQREGAEVVHLGHNRPVH
                     EVVIAALQEDADGIAISSYQGGHNEFFKYIYDLLKE"
     gene            complement(254..988)
                     /gene="pgsA"
                     /locus_tag="PLF85_12345"
     CDS             complement(254..988)
                     /gene="pgsA"
                     /locus_tag="PLF85_12345"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNE20506.1"
                     /translation="MLAWLTLPNFLTIMRIVMIPFFLYYLLSQHPRDRLIALAIFGIA
                     SVTDFFDGYLARKWKQESSLGRFLDPLADKALVIATLVIFILLDQAVPLWMVIVIILR
                     DMLVTLMRYLAIRKGTEIRTTRLGKAKTMFQMFSIVLILVILIMRSFHFDIRATYSEG
                     RAAGLKNYQIATTLLMKATQSFQSAEIPKAERNKYFAESAPYFLLLLTTIITAISGLR
                     YLYTNWRVLLPPYTAILRPRPPRAKP"
     gene            complement(1070..2482)
                     /locus_tag="PLF85_12350"
     CDS             complement(1070..2482)
                     /locus_tag="PLF85_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20507.1"
                     /translation="MADDLQLDTKIPLGLAHDGQILWISDAQTRQLTGYDVGQKKRLA
                     TRTLAYDVRDLAFWAPNLVTIAPNYVLVINPINGDLVDKIPLKGIADPVAIALDMHQA
                     YIYNREDKKIYRVHLVDRMQFGSFTPEISSDIRSMTFYKGFLWAVAKDGKAYKISPSD
                     GGQVSFLPLPEGCYGISFVDGGMYVARPGQVRSVDFIETESYVAAARRNFTLAAELQL
                     ELPWNEEERAREARLQLKYSLLPVTAHQRIAGLRADPFTRFGRWEDGSHAAELTLEPK
                     SSEKKRTYRLQFQATIYNLTHIFNAQLIKQYFRNPELPDSVRPYLDALPLDAGRMAVI
                     DTFRNSWLQKNDGKHPIYAIAALKADKAMDDASRSGAIRALGVPCRKMVFLDLDSKKT
                     MTWLQVYLQPTGWVTISDKYDPAKPKEFPVANNELELFSPDDIALLPRLRRSAGAAAA
                     TTPGELLKFTNVQVITSSTE"
     gene            complement(2551..4017)
                     /locus_tag="PLF85_12355"
     CDS             complement(2551..4017)
                     /locus_tag="PLF85_12355"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNE20508.1"
                     /translation="MAIHTPAMYRVGYLNLFVALLIFGLAPLAAEKAKVQPEDRPWYH
                     YIREEMPEAKIPVVDRFRLNNGIEVYYLKSDTVPLVHMQVIIDGGGFEVPDDKLGLHN
                     LWGDTVVFSGSDKMNRDGLSNYLESRASAFSFSAGAERSTFNLRSMAHYFERDLQTVF
                     EVLQKPRLAGDDFELLRKRVLQELERRDENPAKWAALGMSRLYWGDTLRGRYATRRTV
                     AALKTEDLRAWHDRVWRGERLSIAVSGSIEAPRLRKLLNETLGKLPVQPKDAPDLKKM
                     HVSPIAKGNELRMLPKEIPQTTVIYRAPGMKHTDPDYYALRILDFLLGGDSFNSYLTQ
                     KIRTEKGWAYTAYSSFETDDFTGSLVLFTQTANTSLPDVLALIDEILAQPKQYIDAKK
                     IEQAKLSLRNKFVFLFENPAQYMRLYLQLKWDGLPDSYLTNYAKNLRKVTEADVLRVA
                     RKYYRPENFTVLLCGPKDVYQKKSALRPESAQVLELEK"
     gene            complement(4017..4778)
                     /locus_tag="PLF85_12360"
     CDS             complement(4017..4778)
                     /locus_tag="PLF85_12360"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE20509.1"
                     /translation="MRTLIATLLITTAGFAQEKPKELLAAALSVSGECFYERDGKTLP
                     AKPKTIFFRNDRLFTKKGKMDVQIGPVAVLHLAPYSTIRLSDLTEQDRKTNIVVDLES
                     GRGYTKFATKMGPGSKYTIKSPTLVAGIRGTEFVLSAGDESPQPHEDSDIPQGVFVNT
                     GVVAVSPASREEEVIELQPGEQVTGVDNALVKGVMEDFIKKKMKLFKQLNAMKEEQYR
                     ILEREKNRQIELLEKVKGSVNIDQLKEKNKQLFNK"
     gene            complement(4841..>5070)
                     /locus_tag="PLF85_12365"
     CDS             complement(4841..>5070)
                     /locus_tag="PLF85_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20510.1"
                     /translation="AVTDMSVTASSATMQPLAVYSRSGNGTTAGNGAVTFLLPSGTYR
                     YFCTLGASSSSGNLTVTGATTTALGATDFTP"
BASE COUNT         1094 a         1296 c         1449 g         1231 t
ORIGIN      
        1 ttccttcagc aggtcataga tgtatttgaa aaattcgttg tgcccaccct ggtacgagct
       61 gatcgcaatg ccatcggcgt cttcttgcag agcagcgatc acaacttcgt gcacggggcg
      121 attatggccg aggtgtacga cttcggcgcc ttcgcgctgc aaaaagcggc ggatgatatt
      181 gatcgaggcg tcgtggccgt caaaaagcga cgtcgcagtg acaaatcgga ctggtttcat
      241 aggtttcctt agattagggt tttgcccttg gcgggcgtgg tctgagaata gcagtatagg
      301 gcggtaagag aacgcgccaa ttggtgtaga gatagcgcag gcctgaaatc gcggtgatga
      361 tcgtcgtgag aagaagcagg aagtaggggg cggattctgc aaagtacttg ttacgctccg
      421 ctttggggat ttctgctgac tgaaacgatt gtgtcgcctt catgagcagt gtcgtcgcaa
      481 tctggtagtt tttcagtcct gctgcccggc cctcgctgta cgttgcgcgt atgtcgaaat
      541 ggaacgaacg catgatcagt atcacgagta tcaggactat cgagaacatc tggaacatgg
      601 tcttggcctt gcccagccgt gtagtgcgta tctcggttcc cttgcggatt gccaggtagc
      661 gcatgagcgt gacgagcata tcccgaagaa tgatgacaat gaccatccag agcgggacag
      721 cctggtcgag caaaatgaag attaccagtg tggcgatcac cagcgctttg tcagccaggg
      781 ggtccaggaa ccgtccaagc gaagactcct gtttccactt tctcgcaaga taaccgtcaa
      841 aaaaatcggt aaccgaagct atgccaaata tagccagtgc aatcaaccgg tcacgcggat
      901 gctgagacaa aagataatac aggaaaaagg ggatcatcac gatacgcatg atcgtgagaa
      961 agttcggcaa ggtgagccat gccagcattg ggttcaaatg agtttctgag acgtttgctc
     1021 aagtagattt cggtgctctc gcgcctttgt gcaggcccgc gggtcgaagc tattccgtac
     1081 tgctcgtaat cacctggaca ttggtgaatt tcagcaattc gccgggtgta gtcgcggctg
     1141 cggcgcctgc agagcgcctg agccgtggca gcagggcaat atcgtccggt gagaatagtt
     1201 cgagttcatt gttggccacc gggaactctt tgggtttcgc gggatcatat ttgtcagaaa
     1261 tcgtcaccca accagtgggc tgcaggtata cctgcagcca ggtcatcgtt ttcttgctgt
     1321 cgaggtcgag gaataccatc ttacggcagg gcacacccag agcccgaatg gcgccggagc
     1381 ggctggcgtc gtccatggct ttgtcggcct ttagggctgc tatcgcatag atgggatgct
     1441 tgccgtcgtt cttctgtaac cagctgttgc gaaaggtatc aattacggcc attcggcctg
     1501 cgtcgagtgg cagcgcatcg aggtatggcc tgacgctgtc gggcagttca ggattgcgga
     1561 agtactgctt gatgagctgt gcgttgaaga tatgcgtcag gttgtagatg gttgcctgga
     1621 actgcaggcg gtatgtgcgc ttcttctcgg agcttttcgg ttcaagagta agctccgccg
     1681 cgtgcgagcc gtcctcccag cgaccaaaac gcgtaaaagg gtcggcgcgc agtcccgcga
     1741 ttcgctggtg cgccgtcacg ggcagcaacg aatattttag ctgtaggcgc gcttcgcggg
     1801 cgcgctcttc ttcgttccac ggtaactcga gctgcagctc ggctgcgagc gtaaaattgc
     1861 ggcgcgccgc agctacgtaa ctttctgtct cgataaaatc gaccgaacgc acctggcccg
     1921 gccgggcgac gtacattccg ccgtcgacaa aactgattcc ataacagcct tcgggcaacg
     1981 gcaaaaagct gacctggcca ccgtcgctgg gagaaatttt gtatgccttt ccgtctttgg
     2041 ctacggccca gagaaacccc ttgtaaaagg tcatgctgcg gatgtcgctg gaaatttcgg
     2101 gtgtaaacga accgaactgc attcgatcaa ccaggtgtac gcggtagatc ttcttatctt
     2161 cgcggttata gatatatgcc tggtgcatat cgagcgcaat ggcgacaggg tcggcaatac
     2221 ccttgagcgg aatcttatct acaagatcac cgttaattgg gttgatgacg agaacatagt
     2281 tgggcgcgat ggtaacgaga ttcggagccc agaacgccag gtcgcgaacg tcatatgcca
     2341 gggtacgcgt tgccaggcgt tttttctgcc cgacgtcgta gccggttagc tggcgcgtct
     2401 gtgcgtcgct gatccagagt atctgtccgt cgtgggcgag cccgagaggt attttggtgt
     2461 ccagctgcag atcatcagcc aagaggggat ttaaaaacaa gaggaggaag actccaagac
     2521 cacggcagaa aggtcctggc tgggaaaact tcatttctcc agttccagta cctgtgcgct
     2581 ctctggacgc agcgcggatt ttttctggta aacatctttt gggccgcaga gcaacacggt
     2641 gaagttttcc gggcggtaat atttgcgcgc gacgcgcagt acatcggctt cggtgacttt
     2701 gcgcaaattc tttgcatagt tggtaaggta agaatccggc aggccatccc acttgagctg
     2761 caggtagagc ctcatgtact gcgccgggtt ttcaaacaaa aagacaaact tattgcgcag
     2821 cgagagtttt gcctgctcga tcttctttgc gtcaatgtat tgctttggct gtgccagaat
     2881 ttcgtctatc agcgcgagca catcaggcag gctggtattt gctgtctgag taaacaatac
     2941 gaggctgccc gtgaagtcgt cggtctcaaa ggatgaatat gccgtgtacg cccagccttt
     3001 ctccgtgcgg attttctgtg tcaggtacga attaaacgaa tcacccccga gcagaaaatc
     3061 gagtatgcgc agtgcatagt agtcgggatc cgtgtgtttc attccggggg cacggtaaat
     3121 cactgtcgtt tgcggaatct ctttaggcag catgcgcagc tcattgcctt tagcgatcgg
     3181 cgaaacgtgc attttcttca ggtcaggcgc atctttcggt tgcacgggga gttttcccag
     3241 tgtttcattg agcagtttgc gcaggcgggg agcttcaatt gagccactga ctgcaatact
     3301 caggcgctct ccgcgccaga cgcggtcatg ccacgcgcgc aggtcttcag ttttgagggc
     3361 tgcaacggtg cggcgcgtgg cgtaacgccc gcgcaatgtg tcgccccagt agagccggct
     3421 catgccgagc gctgcccatt ttgccgggtt ttcgtcgcgg cgttcgagtt cttgcagtac
     3481 cctcttgcgc agcagttcaa aatcatcgcc agccagccgc ggtttctgca gaacctcgaa
     3541 cacagtctgc aggtcgcgct cgaagtaatg cgccatggag cgcaggttga aagtcgaacg
     3601 ttcagcacct gcgctgaagc taaaggccga ggcgcggctt tcaagataat tagataaccc
     3661 atcacggttc atcttgtcgg agcccgagaa gactaccgtg tcgccccaca ggttgtgcag
     3721 accgagtttg tcatcgggta cttcgaagcc cccgccatct atgatgacct gcatgtggac
     3781 taaaggtaca gtgtctgatt tgaggtaata aacttcgata ccattgttga gccgaaaacg
     3841 atccaccacg gggattttcg cttcaggcat ctcttcgcga atgtagtggt accagggcct
     3901 gtcttcgggc tgcactttgg ctttttcggc agcaagcggc gctagcccga aaattaacag
     3961 cgcgacaaac aggttcaggt aacccacgcg gtacatggca ggagtatgga tagccattat
     4021 ttgttaaaca gctgcttgtt cttttctttg agctggtcga tattaaccga acctttcact
     4081 ttttcgagca gttcgatctg ccggttcttc tcgcgctcaa gaatgcggta ctgctcttct
     4141 ttcatcgcgt tgagttgttt gaaaagtttc atcttctttt tgataaaatc ttccatgacg
     4201 cctttgacca gggcattatc aacgcccgtt acctgttcac ccggctgtag ttcaatgact
     4261 tcttcttcgc ggctggctgg cgaaacggcc acgacccccg tgttgacaaa aactccctgt
     4321 ggtatgtctg aatcttcatg cggttggggc gattcatcgc ctgcactcag cacgaattcg
     4381 gtcccccgaa tgcccgcgac aagcgtcggg ctcttgatcg tatactttga acccgggccc
     4441 attttcgtgg caaattttgt ataacccctg cctgactcga ggtcgaccac gatgttcgtc
     4501 ttgcggtcct gttctgtcag gtcgctcagc cgaatggttg aatagggggc aaggtgcaga
     4561 acggcgactg gaccgatctg cacgtccatc ttacccttct tggtgaacaa ccggtcgttg
     4621 cgaaaaaaaa tcgtctttgg tttcgcgggc agcgttttgc cgtcgcgttc atagaaacat
     4681 tcgcctgaaa ccgaaagcgc ggcggcgagc agctctttcg gtttctcctg ggcaaagccc
     4741 gctgtggtga tgagaagggt ggctattagg gtgcgcataa ggtctcctgc accctattgg
     4801 acgtttctca ggccgaaaag ggaaaaaatt cagccgggag ttacggggta aaatcggtcg
     4861 cacccagtgc ggtggttgtg gcgcccgtga cagtcaggtt acccgagctg ctgctggcac
     4921 cgagggtaca gaagtagcgg taggtacccg aggggagcaa aaaagtgacg gcaccatttc
     4981 ctgcagtagt accattgccg ctacggctgt agaccgccag cggctgcatc gtcgcagagg
     5041 atgctgtcac cgacatatcc gtcaccgcac
//
LOCUS       DAOQJF010000249         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9699_length_5061_cov_5.609469, whole genome shotgun sequence.
ACCESSION   DAOQJF010000249 DAOQJF010000000
VERSION     DAOQJF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_5061_cov_5.609469"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..915
                     /locus_tag="PLF85_12370"
     CDS             37..915
                     /locus_tag="PLF85_12370"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE20511.1"
                     /translation="MPIAAVIVTYNPDDKFGERLAAIARECGQVYVVDNGSTNQAALK
                     KITKRPRVKLLALGQNTGIAHAQNVGLSAAFESGAEAVILFDHDSAPRPGFVSALRSA
                     AGTSRIAGPRIYDINKKSFALHPCYAGLFFRRRPAPESGVLAGAMMVIASGCLVPRAV
                     YERVGGMREEYFIDYVDWEYCLRARHRFGVETVVAGAAILDHARGERTGKKVGPLTVY
                     PPGYSAFRYGHIFRNRATLLREYLFKDRAFGAFEFVSLMRDFLLLAAEPRPASLCWLA
                     LKSWFGGLFRRQKAPK"
     gene            991..1323
                     /locus_tag="PLF85_12375"
     CDS             991..1323
                     /locus_tag="PLF85_12375"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNE20512.1"
                     /translation="MPLNPETIEIRLPKKALSGNVAEIAWSDQHKSMHTSFNLRMRCP
                     CATCRDSHNPDPKAIRGPKGVPPMVEIASFDWVGNYAVNFIFNDGHNTGIYTYKYLRE
                     IDESANGV"
     gene            1313..3520
                     /locus_tag="PLF85_12380"
     CDS             1313..3520
                     /locus_tag="PLF85_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20513.1"
                     /translation="MASEALQALRRLRAIGTGLFARQQLRLLAYLGLGLLLWQVLDFL
                     PMLFFNSKAFQAKLEQYIDASRLAISYQGIDVSLFRGVRIIGVRVSFDRDFSRGRYLL
                     EAPAVYIRKPLSFIGAEESALPEGARVIIEDGKIGYWVTSDSADRELVQQVRNLFQKD
                     RRYHIECDSCRFSLNVKDNSYFQEATPVEHLSFTLHHGGKEVQTLVRYESSAIGSGDF
                     FGRFDACSSMQCDDLEGYWYFKPSNLRLAILNNFQKDIDITSGTASGEMAFDRKLTDI
                     VKKVRGKEVTVREPLSNFRMAMSSRNFAVRRKKLPWYEVDAFSIDTRMQIKGTSSTGY
                     VRGALDDYKIQAEFEDLRPEALPEKYLFRIEPEIFGEKRLNLPAHRVLKGLRGLSINL
                     SGRKGNKYTKSEISLDIANGAFIAGENLTPLLLPAVQVNLTDEKLSGQVRALAGSSLF
                     SADLSGAVELYPVQFVPLLDALLREAAGAEERTIFSLRGKVICPLTVETLNWADIRPF
                     VDAWLKDYWEEVQEGIQYSWLPSRLRRREYFVRFIQYLDFSMPIDIKRFAWGAEMPLK
                     GSFFFSPLYGGGGFKLESPDGKNSTSLMVSYGSNEPNAPYMSHNLNLNLEFAYELLSP
                     WFGDEYFEYFSSAQITHFNNFMGERPADHYLKSVSLTDMRLTRVRLGKWARAQALPLQ
                     WETVDVKTNRSNGNGAISLIRAENDNNVLSGYGEYKLFDRQIDTSLKYSVQMR"
     gene            3608..4570
                     /locus_tag="PLF85_12385"
     CDS             3608..4570
                     /locus_tag="PLF85_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNE20514.1"
                     /translation="MTDVLAPNLEKKATRDGYGAALVEIGASNPKVVVLDADLSGSTK
                     TKKFSEKYPDRFFNYGVAEQNLVGQAAGFALSGLIPVASSFAMFAAGRAWEIFRNSVC
                     YPHLNVKVAATHAGITLGEDGASHQIIEDLALTRVMPGLAVLVPSDYNQAAAAIKTAV
                     AFDGPVYLRLGRPNVPSIYSADDVVRVGGSNVLQTGSKAVFIAAGVMVFEAWKAAALI
                     EKETGTKVTVIDAYSVKPLDEATILKATADAQLVVTFEEHNILAGLGGAVAELLAEKQ
                     PRRVHRIGLRDEFGQSGDSATLMKHYKLTAETMLPDLLALLKAI"
     gene            4665..>5061
                     /locus_tag="PLF85_12390"
     CDS             4665..>5061
                     /locus_tag="PLF85_12390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002153084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE20515.1"
                     /translation="MRIAIFTDSFFPKIDGIAVSVQNFCEKLEQRGHKFIICCPRYAD
                     DDAELIGQNIRIERFKSVALPSYGEMRIVVPGRKKLHKVMKDFRPDLVHIQTPGFMGQ
                     YGVLAAKLYSVPVIGTYHTMMSEVGMYVNP"
BASE COUNT         1101 a         1392 c         1409 g         1159 t
ORIGIN      
        1 ttattttcgt ggcaaagcca cgaaaataaa taaacagtgc ccatcgccgc tgtcattgtc
       61 acctataatc cagacgataa attcggcgag cgcctggcgg cgattgcgcg cgaatgcggt
      121 caggtctatg tcgtcgacaa cggcagcaca aaccaggcag ccctgaaaaa aattaccaaa
      181 cgccctcggg tcaaactcct ggcgctgggg caaaacaccg gtattgcgca tgcgcaaaac
      241 gtgggattat ctgcggcatt cgaatcaggt gccgaagcgg tgattctttt cgaccatgac
      301 tctgcgccac ggcccggttt tgtgagtgcg ctccgttctg ctgcgggcac ctcgcggatt
      361 gcggggccgc gcatctatga cataaacaaa aaatcttttg ccctgcatcc gtgttatgcc
      421 gggttatttt ttcgccggcg tccggcgcct gaaagcggag ttcttgccgg tgcaatgatg
      481 gtcatcgcct caggctgcct tgttccgcgt gctgtctatg agagagtggg cggcatgcgc
      541 gaggaatatt tcatcgacta tgttgactgg gaatactgcc tgcgcgcacg ccatcggttt
      601 ggcgtcgaga cggttgttgc aggcgcggcg atccttgatc acgcacgtgg cgaacgcact
      661 ggaaagaagg tcgggccact cactgtctat ccacccggtt attcggcttt tcgctacgga
      721 catattttcc gcaaccgggc gaccttgctg cgtgaatacc tgttcaaaga ccgtgccttc
      781 ggcgcatttg aatttgtttc gctgatgcgg gattttctcc tgcttgctgc cgaaccacgg
      841 cctgcctcac tctgctggct ggcgctcaag agctggttcg gcggtctgtt ccgcaggcaa
      901 aaggccccga aataggggtt gcgggttgct cttgcagata cggccttttc ttgacggagc
      961 gatttcgcct cggggttcta cccgcaacct atgccgctga atccggaaac cattgaaatc
     1021 cgcctgccga aaaaggcgct gtctggcaac gttgccgaaa ttgcgtggag cgatcagcac
     1081 aagagcatgc acacctcatt taacctgcgc atgcgctgcc cttgtgcaac ctgccgcgat
     1141 tctcacaacc ccgaccccaa ggcgatacgg ggtcccaagg gagtgccacc gatggtcgaa
     1201 atcgcttcgt ttgactgggt tggcaactac gcggtaaatt ttatctttaa cgatggccac
     1261 aataccggca tttacaccta caagtacctg agagaaatcg acgaatctgc caatggcgtc
     1321 tgaggcgctg caggcactgc gtcgccttcg ggcgatcggc accggtcttt ttgcgcggca
     1381 gcagctgcgc ctgcttgcct acctcggcct gggtcttctg ctctggcagg tactcgattt
     1441 tctacccatg ctgtttttca acagcaaagc ctttcaggcc aaactggagc aatacatcga
     1501 tgcgagccgg ctggcgattt cgtaccaggg cattgatgtc agtctttttc gcggcgtccg
     1561 tatcatcggc gttcgcgttt ctttcgaccg cgacttttcg cggggccgtt acctgcttga
     1621 agcgccggcc gtctacatac gcaagccact gtctttcata ggcgccgaag agagcgcact
     1681 gccagaaggg gcgcgcgtca ttattgaaga cggcaagatt ggctattggg ttacttcaga
     1741 cagcgccgat cgcgaactgg tgcagcaggt acgcaacctt ttccagaaag accggcgcta
     1801 ccacatcgag tgcgacagtt gccggttcag cctgaacgtc aaagataaca gctatttcca
     1861 ggaggcaacg cccgttgagc acctcagctt cactttgcac catgggggaa aagaagtaca
     1921 gacactcgtg cgctatgaaa gttcggcgat cggtagcggc gattttttcg gccggttcga
     1981 tgcgtgtagc tcgatgcagt gcgatgatct tgaaggctac tggtatttta agccctcgaa
     2041 cctgcggctg gcgattctta acaacttcca gaaggatatc gatattactt ccggcacggc
     2101 gtcgggagaa atggccttcg acagaaagtt gacggatata gtaaaaaagg tccgtggaaa
     2161 agaagtcact gttcgcgaac cactctcgaa tttccgcatg gcaatgagca gccgcaattt
     2221 tgccgtgcga cgcaagaaac tgccctggta tgaagtcgat gcgttcagca ttgatacgcg
     2281 catgcagata aagggaacca gtagtacagg atatgtgcgc ggtgccctcg acgattacaa
     2341 gatccaggcg gagtttgaag acctgcggcc cgaggcactg ccggaaaaat accttttccg
     2401 catcgaaccg gaaattttcg gtgaaaagcg actcaacctt cccgcgcacc gggtgctcaa
     2461 gggattgcgc ggtctttcaa tcaacctgag cgggcgtaag ggtaataaat acacgaagag
     2521 cgagatatct cttgatatcg ccaacggtgc ctttatcgcc ggtgaaaatc tgaccccgtt
     2581 gctgctgccc gcggtgcagg tgaacctgac cgatgaaaag ctgtcaggcc aggtgcgcgc
     2641 ccttgcggga tcgtcgctgt tcagtgctga cctttcaggc gccgtggaac tttacccggt
     2701 gcagtttgtg ccgctgctcg atgcgctgct gcgtgaggct gcaggtgccg aggagcggac
     2761 gatcttctct ttgcgcggta aggtgatttg tcccttaacg gtcgagacgc tgaactgggc
     2821 cgatatcagg cccttcgtcg acgcctggct gaaagattac tgggaagaag tgcaggaggg
     2881 tatacagtac tcctggttac cttcgcggct gcgccggcgc gagtattttg tgcgttttat
     2941 ccagtacctc gatttttcca tgccgattga catcaagcgt tttgcctggg gcgcagagat
     3001 gccgctcaag ggaagtttct ttttctcacc gctgtacgga ggaggcggct ttaagcttga
     3061 atcaccggat ggcaagaact ccacctcgct catggtgtct tatgggagca acgagcccaa
     3121 cgcgccttac atgagccaca atctcaacct aaaccttgaa ttcgcatacg aactgctttc
     3181 accctggttc ggcgatgaat acttcgagta tttttcttct gcacagatta cccatttcaa
     3241 caacttcatg ggtgaaaggc ctgcggacca ttacctgaaa tcggtgtcgc tcacagacat
     3301 gcgcctgacg cgtgtcaggc tgggcaagtg ggcgcgggcg caggcgctgc ccctgcaatg
     3361 ggagaccgtt gacgtcaaaa ccaaccgcag caacggtaac ggggcaattt cgctgatcag
     3421 ggccgaaaac gacaacaatg ttctctctgg ttacggtgag tataagctgt tcgaccggca
     3481 aatcgatacg agcctgaagt actccgtaca gatgcgttag caggctgttt aggctcaccg
     3541 cgatgactgc agggctgccg aaattcgagt gacgccgggt taaaataccc caattagccc
     3601 tgtgccgatt acagatgttt tagctcccaa cctcgaaaag aaagcgaccc gcgacggtta
     3661 tggtgccgcg ctggtcgaga tcggggcttc aaacccaaag gtagtggtcc tcgatgctga
     3721 cctcagcgga tcaaccaaga ccaagaagtt cagcgagaaa taccctgatc gtttcttcaa
     3781 ctacggtgtc gcagagcaga acctggtcgg tcaggccgcc ggttttgccc tttcgggtct
     3841 tattcccgtg gcatcgagtt ttgccatgtt cgccgcgggc cgagcctggg aaatcttccg
     3901 taactccgtc tgttacccac acctcaacgt gaaggtcgcg gcaacgcacg ccggtatcac
     3961 gctgggcgaa gacggcgcaa gccaccagat catcgaagac ctggctctga cgcgcgttat
     4021 gccgggcctg gcagtgctag tgccgtccga ctataaccag gcggcagcag cgatcaagac
     4081 agccgtggca ttcgacggcc cggtctacct gcgtctcggc cgccccaatg tgccctcgat
     4141 ctacagcgca gacgatgttg tccgtgtggg gggcagtaat gtcctgcaga ccggcagcaa
     4201 ggccgtcttc atcgcggccg gtgtcatggt cttcgaagcc tggaaagcag cagcgctcat
     4261 cgagaaagaa accggcacca aagttacggt gatcgacgcc tattcggtta agcccctcga
     4321 cgaggcgaca atcctcaagg caactgccga cgctcagctg gtcgttacct ttgaagagca
     4381 caatattctt gctggcctcg gcggggcagt ggctgagctt ttggcagaaa aacagccgcg
     4441 ccgcgtgcac cgtatcggcc tgcgcgacga attcggccag tcgggtgact ctgcaaccct
     4501 catgaagcac tacaagctca cggcagaaac catgctgcca gacctgctgg ctctgctcaa
     4561 ggcaatctga ttctgaactt tctacctgct atggaattcc atagcaggta gctgtaagtc
     4621 cttgactgcg tgtgtttagt tcgttattta gcaaaatata gattatgcgg attgctattt
     4681 ttactgactc ctttttcccc aagattgacg gcatcgccgt ttcggtacag aatttctgcg
     4741 agaagctcga gcagcgtgga cacaaattca ttatctgctg cccgcggtac gccgatgatg
     4801 atgccgagct gattggtcag aacatccgta ttgagcgctt caaatctgtt gcgctgcctt
     4861 cttacggcga gatgcgcatt gtggtgccag gcagaaagaa actgcacaag gtcatgaaag
     4921 atttcaggcc cgacctcgtg catatccaga cgcccggttt tatggggcag tacggtgtgc
     4981 tcgccgcgaa actttattcc gtgccggtca tcggcacgta ccacaccatg atgagcgaag
     5041 tgggcatgta cgtgaacccg t
//
LOCUS       DAOQJF010000250         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9701_length_5061_cov_4.476628, whole genome shotgun sequence.
ACCESSION   DAOQJF010000250 DAOQJF010000000
VERSION     DAOQJF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9701_length_5061_cov_4.476628"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLF85_12395"
     CDS             <1..342
                     /locus_tag="PLF85_12395"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE20516.1"
                     /translation="FTRENGRTRLSHADGDIEVDAAIVAVGFRPRTALAAAAGLNVNR
                     GIVTDDQFRTSVAGIYAIGDGAEVCGNLYPFVAPIRAQVKHLAGSLLGTVSGGWLAPA
                     VKVRVKVHGFA"
     gene            447..857
                     /locus_tag="PLF85_12400"
     CDS             447..857
                     /locus_tag="PLF85_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNE20517.1"
                     /translation="MKTRAELVDFLKSRGLRISLPKLEIAAHIFKSHRHFTAEEIFRE
                     INEEYPKVSRATVFNVLNFFVEHGLLKKVEARSDVQLFDSNTEDHDHVVVDATGKIFD
                     VQIPEREKLGFLEQIWAKNPHLPRRRDASVVVHV"
     gene            969..2318
                     /locus_tag="PLF85_12405"
     CDS             969..2318
                     /locus_tag="PLF85_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNE20518.1"
                     /translation="MASPATVLLPDSRARTLWDFFLFLVTLTMAVLGPIELVYKLRGK
                     VFLVYSGIFLTCVFLIDMILNFRTAYYYRGEIVDDPKQITRHYLRTWFIVDFVSLFPF
                     DLITGHILVGLSPYLVDIAPALRLARLVRLLHLKELINRWRHADFLNPSMMRLGILLL
                     WMLMVAHWAACGWIVLGNVKPEYNAAENYLRAFYWVITTFATIGYGDITPANIPQIAY
                     TICVEIVGVGMFGYMIGNIASLLANLDIARSKYQEKINRLNLFLEYRDIPIALRQKLR
                     HYYRYMWESRRGYDENLILKDLPSALQKELAMHIHADVIEKVPIFKGASDAFIKEIVM
                     KLTPAMFTPGDFIFREGEIGHNMYFISRGSVEVLSERTNQVYATIGEGGYFGEIALLL
                     AQPRNASVRAVEYCDLYTLDKDSFQVVLGEFPQFAAQVKKMARERMHVPRKRRAKRS"
     gene            2349..3473
                     /locus_tag="PLF85_12410"
     CDS             2349..3473
                     /locus_tag="PLF85_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20519.1"
                     /translation="MSTDRRPVRLSTRRTVVSLLGSLAVAPVFLLCASLYFQTTDFVY
                     GPLAYESNESLVRLIRWSIFGGLTVVLLAIIVWLKTSHPHYGNNPYSLLILLPLLAFV
                     VFSSWIFGIIVFGILIVALLFSQLYVVVVRDELRTPAAGWGKWPDRMATLRFIILAAA
                     AGMGIWLMFTARSWAYRSRQLEEIQRNGLLSQPVSLQQISDGDHKEYVLLASGNLRVD
                     YRADRKFGSGDDSYTLEYGVLTDRILDKPAILDWRLWVYMPAFYKPTQFPMIFQAVRC
                     TVGKDCPSNFVTMHDAIIRRYFPDTRYREATQGLIAKEGQVLVELTPEPDLVLGDEAW
                     WRMISWRASVLLLLQALYSLAHLFELLLIHAKLKAAYPEA"
     gene            complement(3470..>5061)
                     /locus_tag="PLF85_12415"
     CDS             complement(3470..>5061)
                     /locus_tag="PLF85_12415"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNE20520.1"
                     /translation="SAVQRAGRAGRLGPGTCYRMYTEADFRRRPKTRAPEILRTDLAE
                     ASLRTLAFGSPLKSLMLPDMPSAAMIAQAERLLHDLGLIDKHSQLTPLGKKAATLPLH
                     PRLATMLAASPSEDTALLAAMLSERDAFTNPTPSPSPYQGEGRRGGVATDLEIRFQSL
                     QRFIETGEVPAGADRRGLEAIARVFKHLRGKSNPTPGPSPDSGEGSRRRGGVLAPLLF
                     AFPDRVAVRRDQGSFLMRNGRGVYTEKGDWLAESEFIVAVDVSGAEKNAKLRLGLSVS
                     RKQVMEHFAAAIEKRNEIREVAGKPRAYEQACLGAIVLVEKETALPEGFAEKLVLQKL
                     REGDFWGFLGEESRSLIARIELMRSFGADLPESSPELLQGTLDEWLAPFVAGARTLDD
                     VTDNKIAEALLARLSYQQQKLLEDNLPAKLVVPSGSAHRIRYEDGKAIVAVRIQEVFG
                     MKTHPSLAGGKLPVTFELLSPGRQPIAITKHLPELWKSTYSEIRKEMRGQYPKHFWPE
                     DPLSMQGTTGTKKAFDRKKQS"
BASE COUNT         1055 a         1435 c         1382 g         1189 t
ORIGIN      
        1 ttcacccgcg agaacggtcg aacgcggctg agccacgcgg atggcgacat cgaggtcgat
       61 gcggcgattg tcgccgtcgg cttccgaccc cgtacggcgc tcgccgccgc agccggcctc
      121 aacgtgaacc ggggcattgt gacagacgac cagtttcgca cctcggtcgc cggtatctat
      181 gccatcggtg acggtgccga agtctgcggc aacctctacc cgtttgtcgc tcccatcagg
      241 gcgcaggtga agcacctggc gggttcactt ttgggtacgg tatctggcgg ctggttagcg
      301 cccgcggtca aggttcgggt caaggtgcat ggttttgcgt agatttgacg gtcaggggta
      361 tagctccgtc aaagtagcaa atcccgctgt gtgaaaaaat agacaagctc taaatatatg
      421 gttaacttca aaggcagaga gattttatga agacacgggc agaactggta gattttctca
      481 agagccgcgg cctgagaata tctttgccca agctcgagat tgcggcgcac atcttcaagt
      541 cgcaccggca ctttaccgcc gaagaaatct tccgagaaat caacgaagag tatcccaagg
      601 tctcgcgcgc gacggtcttc aacgtactga acttctttgt cgagcacggc ctgctcaaaa
      661 aggtcgaagc gcgcagcgac gtgcaactgt tcgattcgaa taccgaagac catgaccacg
      721 tggttgtcga tgcaaccggc aagatctttg acgtgcagat tcccgaacgc gaaaagctcg
      781 gctttctcga gcagatctgg gccaagaacc cgcacctgcc ccggcgccgc gacgcatcgg
      841 tcgttgtgca cgtctaacat cccactcggg cataatcaac ccgcacgctt cagtgcggat
      901 gttagtccgt cgaagataaa atagtaccag tgaaggccgt gtgcttagtc accggttatg
      961 tcacatccat ggcctctcct gcaaccgtat tactgcctga ttcacgcgcc cgtactttgt
     1021 gggatttctt cctgtttctc gtgacgctca cgatggcggt gcttggcccc atcgaactcg
     1081 tctacaagct ccggggcaaa gtttttctcg tctattcggg aatatttctt acctgcgtct
     1141 ttctcatcga catgatactg aattttcgta ctgcgtacta ctaccgcggt gaaattgtcg
     1201 atgatccgaa acaaataacc cggcattacc tgcgcacctg gttcattgta gactttgtct
     1261 cactttttcc attcgatctg attacgggcc acattcttgt cggcctgtca ccttacctgg
     1321 tcgacattgc ccccgcgctc aggctcgcaa ggctcgtgcg cctcttgcat cttaaagaac
     1381 tgatcaaccg ctggcgccat gcagattttc tcaacccgag catgatgcgc ctcggcattc
     1441 ttctgctctg gatgctcatg gtggcgcact gggctgcgtg cggctggatc gtgctgggca
     1501 atgttaagcc cgagtacaat gcggctgaaa attatctgcg ggcattctat tgggtgatta
     1561 ccacctttgc caccatcggt tacggtgata tcacgcctgc gaacattccg cagatcgcct
     1621 acactatctg cgtcgaaatt gtcggcgtgg gcatgtttgg ttacatgatc ggtaatattg
     1681 cgagcctgct ggccaacctc gacattgcgc gttcgaagta ccaggaaaaa atcaaccgcc
     1741 tcaacctgtt tctcgagtac cgcgacattc ccattgcgct gcggcagaag ctgcgccatt
     1801 actaccgtta catgtgggaa agccggcgcg gctacgacga aaacctcatt ctgaaagatt
     1861 tgcccagcgc gctgcagaaa gagctcgcga tgcacatcca cgcagacgta atcgagaagg
     1921 tacccatctt caagggagct tcagacgctt ttatcaaaga gatcgtcatg aaactgacac
     1981 ccgcaatgtt caccccagga gactttatct ttcgtgaggg ggaaattggc cataacatgt
     2041 acttcatcag ccgcggcagc gtcgaagtgc tgtctgagag gactaaccag gtttatgcga
     2101 ccatcggcga gggtggttac ttcggcgaaa tcgcgttgct gctcgcgcaa cctcgcaatg
     2161 catcggtgcg tgctgtcgaa tactgtgacc tgtacacact cgacaaagac agcttccagg
     2221 tcgtactcgg cgaatttccg caatttgccg cgcaggtgaa aaagatggcg cgcgaaagaa
     2281 tgcacgtgcc gcgcaagagg cgcgctaaac gcagttgacc ttctgaccga tttcgcagcg
     2341 actgttgcgt gagtacagac cgaagaccgg tacgactgag tacgcggcgc accgttgttt
     2401 ccctgcttgg ttcactcgcc gtggcccccg tatttcttct ctgtgccagt ctctacttcc
     2461 agacaactga ttttgtctat gggccgcttg cttacgagag caatgaaagc ctggtgcggc
     2521 tcattcgttg gagcattttt ggcggtctta cggttgttct gctggcaatt attgtctggt
     2581 tgaagacctc gcatccgcac tacggcaaca acccgtacag cctgctgatt cttttgccgc
     2641 tgcttgcttt tgttgtcttc agcagctgga ttttcggcat aattgtcttc ggcattctca
     2701 ttgtcgcact cctgttcagc cagctttatg tcgtcgtagt acgcgatgaa ttgcgtaccc
     2761 cagcggcagg ctggggtaaa tggcctgacc gtatggcgac gctgcgcttc atcattctgg
     2821 ccgcggccgc aggtatggga atctggctca tgtttacggc gcgctcatgg gcgtaccggt
     2881 cgcgacagct tgaagagatc cagcgtaatg ggctgctatc acagcccgtt tcgctgcaac
     2941 agatttccga cggcgatcac aaagaatacg tcttactggc atccggcaac ctgcgggttg
     3001 actaccgggc cgacagaaaa ttcggttcgg gtgacgatag ctacaccctg gaatacggcg
     3061 tactgacaga caggattctc gacaaacccg cgatactcga ctggagactt tgggtgtata
     3121 tgccggcatt ctacaaaccc acgcagtttc ccatgatttt tcaggctgtg cgctgtaccg
     3181 tcggcaaaga ctgcccgtca aattttgtca ccatgcacga cgccattatc aggcgctatt
     3241 ttcccgatac ccggtatcgc gaagcgacgc aggggctaat tgctaaagaa gggcaggtgc
     3301 tcgttgaact gacacccgag ccagacctgg tactgggtga cgaggcctgg tggcgcatga
     3361 tttcctggcg cgcctcagta ctacttctat tgcaggcgct ctattcactt gcccacttgt
     3421 tcgagcttct gcttatccat gcaaagctca aagctgctta tccggaagct taagactgct
     3481 ttttccgatc aaacgccttc ttcgtacccg tcgtgccctg cattgacaat ggatcttcgg
     3541 gccagaagtg cttcgggtat tggccgcgca tttctttgcg aatttcagaa taggtggatt
     3601 tccaaagttc tggcaaatgt ttcgtgatcg caatcggctg cctgcccggc gagagtaact
     3661 caaacgtgac cggcagcttt ccccccgcca gtgacgggtg cgtcttcatg ccgaagacct
     3721 cttgaatacg cacggcgacg atcgctttgc cgtcttcgta gcgaatacgg tgagcagagc
     3781 cgctgggcac caccagcttg gccggtaaat tatcttcgag caacttttgt tgttggtagc
     3841 ttaagcgcgc cagcagcgct tcagcaattt tgttgtcagt cacatcgtcg agcgtccggg
     3901 cacctgcaac aaacggtgcc aaccactcgt cgagcgtacc ctgcaagagc tcgggcgagc
     3961 tttcaggcaa atcagctcca aaggagcgca tgagttcaat gcgcgcaatg agtgagcgtg
     4021 actcctcacc cagaaaaccc cagaagtcac cttcgcgtaa tttctgcaga acgagctttt
     4081 cggcaaatcc ctcaggcagc gctgtctctt tttcgacaag cacaatcgcc ccgaggcagg
     4141 cctgctcata ggcgcgcggc ttgcccgcaa cttcacgtat ttcgttacgc ttttcgatcg
     4201 cagctgcaaa gtgctccatg acctgcttgc gtgaaacaga aagtcccagc cgcagcttcg
     4261 catttttttc cgcaccgctg acatcgaccg caacgatgaa ctccgattcg gcgagccagt
     4321 cgcccttttc tgtgtaaaca ccgcgaccgt ttcgcatgag aaatgacccc tgatcgcgtc
     4381 gaaccgccac acgatcggga aaagcgaaga gcagtggggc aagaacccct ccccgcctac
     4441 ggctcccctc ccctgaatca ggggaggggc cgggggtggg gtttgatttt cctcgcagat
     4501 gtttaaatac ccgcgcgatc gcttccaatc ctcttctgtc ggcgcccgcc ggcacctcac
     4561 cggtttcgat gaatctctgc agcgattgaa aacgaatttc gagatcggtc gcgacccctc
     4621 cccttcgccc ctccccttgg taaggggagg gggacggggt ggggtttgtg aaggcatcgc
     4681 gttcagagag catcgctgcc agcagagctg tatcttcaga aggtgaggcg gcgagcatgg
     4741 tggcgaggcg cgggtgcagc ggcagcgtag cggctttttt gccaagtggg gtgagctggc
     4801 tgtgcttgtc gatcaaaccg aggtcatgta gcagacgctc cgcctgtgca atcatggccg
     4861 ccgagggcat gtcgggcagc atcagcgatt tcagcggcga accaaaagcg agtgtgcgca
     4921 ggctagcttc ggcgaggtcg gtgcgcagaa tttcgggggc gcgggttttc ggtcgccggc
     4981 gaaaatcggc ttcggtgtac atgcggtagc aggtgccggg gccaaggcgt cccgcgcgtc
     5041 ctgcgcgctg cacggctgaa t
//
LOCUS       DAOQJF010000251         5046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9746_length_5046_cov_4.871769, whole genome shotgun sequence.
ACCESSION   DAOQJF010000251 DAOQJF010000000
VERSION     DAOQJF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9746_length_5046_cov_4.871769"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..498)
                     /locus_tag="PLF85_12420"
     CDS             complement(94..498)
                     /locus_tag="PLF85_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20521.1"
                     /translation="MRRNLLLTISLLLLGGCTYDASPPGEDEINDSKPLLLIDATGLP
                     ARRLIRIVYTDVSGTPKTLATSSEKTDNDGRLLYPLYMLRGTRNYSVTITTDDSNDNQ
                     FGVGDKTTGARNGSFVADGETIILRLVAADFT"
     gene            complement(505..2157)
                     /locus_tag="PLF85_12425"
     CDS             complement(505..2157)
                     /locus_tag="PLF85_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE20522.1"
                     /translation="MSSPTTTAKENPGLKDFDLTGYTGTKGWNFFEETPALWQAFSMN
                     SANSESGYLSAVRDHLSGLGNLAGGIVNELTIECHREGKWGELVQYDRTGKRIDEIRY
                     APEQVELRKIFYDYGVVNLDCKDSWKHEFSIPHRMALAVLTNMNGECGVACPLAMTEG
                     LIHALRAIGTEQQKEEFLPRLTDPASKSHFMAGQYVTERVGGSNVAANRTIATLQSDG
                     TYLLNGEKWFCSNPGDAWVTTAKLAGTNVVGLFLVSRYRRDGSLNGCRLLRKKDIIGS
                     KGKLTVESVYEDCEATALGRPAHGLANLIRYILKTSRLHVICGGLGHIARGVLEAEAY
                     TRSREAYGKKLAEFPSVARTLEEMRMLRDALTFISFRAFALADKDEPLSLLLVPLLKT
                     IVTQYSAVVVKEALILHGGNGILGDFSVLPRVLNDAIINETWEGTHALLAEHAVSAAK
                     RPKVAKAFAEYVDAAAALMPAEAQNLLANEFEETNKLLASEFDCDVNRMYICEKLWRI
                     FALAELDRRAQVYPDSSHHRQLLLMQNFVAGHARMPVRMTEN"
     gene            complement(2163..3083)
                     /locus_tag="PLF85_12430"
     CDS             complement(2163..3083)
                     /locus_tag="PLF85_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNE20523.1"
                     /translation="MAADQVIYKLIPYAVPKPWGGGHLEKLTGNTSAEKIGEYVLFSD
                     LPQFPVRIQVGTSDMPLAEFWAKKMGAGAAVPFMLKVLSTAEPISVQNHPSDDDVKAL
                     GLAGNGKMEAWTILSADPQARMYLGLKGEQSPEVLRQLEELPEPLSVFHEFKPQQGEV
                     VTLSPGLVHSTTGRLLFYEVQQTSDYTFRIYDFGRGRELHLDQAIRCVHDQDPEIADF
                     SSDLITPRFRVSYHEAKSGLVALRSEKSSVITWFGSQAQLSCGSESFSAQWGDSFFIP
                     DAASRAVVIAQQAEAPEGLPMIDMLFEAHL"
     gene            3215..3994
                     /locus_tag="PLF85_12435"
     CDS             3215..3994
                     /locus_tag="PLF85_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE20524.1"
                     /translation="MTATASSGLSRFYRGLLAVALLSLPLVFFYNLELFGWLNCRKDA
                     TVLNYFFLTLTSLADGLWIMMLATVVQSVAPRNFKAFLIALVLGNVFLQSGKYFFDAD
                     RPLRLLGESAICLLGQPLTVRSFPSGHSFSVGLLFMFLRPRHSWVGAFALLAISLLAA
                     VSRAYVGVHFPRDIISGFLLAVLSFLGAEILSRKITFRETSLTLRKGLVAFLGMSIAL
                     LYIFAYHEKTRELEAVLTPAAWVVVLYWCLYAARLLVLRYG"
     gene            4064..4399
                     /locus_tag="PLF85_12440"
     CDS             4064..4399
                     /locus_tag="PLF85_12440"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE20525.1"
                     /translation="MKAAEFHPAALSEIKSYSKEIKSKLGDLILDLQLGESISMPDSR
                     PMPSVGPGVSELRVKDSQNQYRAFYYLKVAGKVIIFHAFVKKTEKTLKREIDIGKKRL
                     KDILEAMNG"
     gene            4392..4691
                     /locus_tag="PLF85_12445"
     CDS             4392..4691
                     /locus_tag="PLF85_12445"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE20526.1"
                     /translation="MGKLNVVTAKNPKELFEAIGLTECEAADAIFRAKLNSDISSLFG
                     KSKLTHEALAKKVGTSRTRITALLNRSRSDISTDFMLKVLAALGYTVEPRLKKIA"
BASE COUNT         1097 a         1316 c         1404 g         1229 t
ORIGIN      
        1 cgtcggcagc gaaaagccgc tggttacggg acgcgccgat ctttccacgc taaaaatcgc
       61 gctggatagc gtgaaacaga ttactggctg atctcaggta aaatctgctg ctacaaggcg
      121 gagaatgatt gtttccccgt ctgctacgaa gcttccattc cgggcgcctg tggtcttatc
      181 tcccactccg aactgattat cattagagtc gtcggtggtt atcgttacgg aataattccg
      241 cgtgccccgc aacatgtaca gcggataaag caggcgccca tcgttatcgg ttttttcact
      301 gctagtggcc agtgtcttcg gtgtaccaga gacgtccgtg tagactatac ggattaaccg
      361 gcgcgccggc aacccggtag catcgatcag caacaacggt ttactgtcgt taatttcatc
      421 ctcgcccgga ggtgaagcgt cgtaggtgca gccaccaagc agcaggagcg atatagtgag
      481 taggaggttt cgtctcatgt ttttttagtt ctcggtcatg cggaccggca tacgcgcgtg
      541 cccggcaaca aaattctgca tcaacaacag ctgtcggtgg tggctggagt cgggataaac
      601 ctgtgcgcgg cggtcgagtt cggcgagcgc aaaaatgcgc cagagttttt cgcagatata
      661 catgcgattt acatcgcagt caaattcact ggcgagcagc ttgtttgtct cttcaaactc
      721 attggccagc aggttctggg cttcagcagg catgagcgcc gcagccgcgt ccacatactc
      781 cgcgaaggcc ttcgccactt tcgggcgttt tgctgccgag acggcgtgtt ctgccagcaa
      841 ggcatgcgtg ccctcccagg tttcgttaat gatcgcgtcg ttgagcacgc gcggcagaac
      901 gctgaaatca cccaggattc cattgccacc gtgcaaaatc agcgcctctt tgacgaccac
      961 tgcactgtac tgcgtgacga ttgtcttcag caggggaaca agcaacagcg acagcggctc
     1021 gtctttatct gccagcgcga acgcgcggaa gctgatgaac gtcagtgcgt cacggagcat
     1081 ccgcatttct tcgagtgtgc gcgccacact gggaaactcc gccagttttt taccatacgc
     1141 ttcgcgagaa cgggtatatg cctcagcctc gagcacgcca cgcgcgatgt ggccaagacc
     1201 accacagatg acgtgcaggc gcgaagtctt gagaatataa cgaatcaggt tggcgagacc
     1261 atgcgcggga cggccgaggg ccgttgcttc acagtcttcg tacacgcttt ctacggtgag
     1321 ttttcccttg gaaccgatga tgtctttttt gcgcagtagc cggcaaccat tcaggctgcc
     1381 gtcgcggcgg tagcggctga cgagaaagag accgacgacg tttgtccctg cgagtttggc
     1441 ggtcgtcacc caggcgtcgc cggggttaga gcagaaccat ttttcgccgt tgagcaggta
     1501 cgtgccgtcg ctctgcagcg tcgcaatggt ccggttggcc gcgacgttgc tgccaccgac
     1561 gcgctcggtg acgtattgcc ctgccatgaa gtgcgatttg cttgccgggt cggtgagccg
     1621 cggcagaaac tcttctttct gttgctcagt gccgatggca cgcagcgcgt ggattaagcc
     1681 ttcggtcatg gccaaggggc aggcgacgcc gcattcgccg ttcatgttgg tgagtacggc
     1741 aagcgccatg cggtgcggaa tactgaattc gtgtttccag ctgtctttgc agtcgaggtt
     1801 cacgaccccg taatcgtaga atatcttgcg cagctcaacc tgttcgggag cataacggat
     1861 ttcgtcgatg cgcttacccg tacgatcgta ttgcacgagc tcgccccatt tgccttcgcg
     1921 atggcactcg atcgtgagct cattgacaat gccgcccgcg agattgccca gccccgaaag
     1981 gtggtcgcgc acggccgaga gatagccgct ttcgctattg gccgagttca tcgaaaatgc
     2041 ctgccacagg gcgggtgtct cttcgaaaaa gttccagccc tttgtgcccg tataacccgt
     2101 aaggtcgaaa tccttcagcc cgggattctc tttggctgtg gtcgttgggc tgctcatagg
     2161 tttcaaaggt gtgcttcgaa cagcatgtca atcattggca ggccttcggg agcttcagcc
     2221 tgctgagcaa taactaccgc cctgctcgct gcatctggta taaagaacga gtcaccccac
     2281 tgcgcgctga aagattcact gccacaggaa agttgtgcct gagagccaaa ccaggtgatg
     2341 acagaggact tttcagaacg cagcgccaca agacctgatt ttgcctcgtg gtagctcacg
     2401 cgaaagcgcg gtgtgatgag gtcgctcgag aaatcggcaa tctcggggtc ctggtcgtgc
     2461 acacagcgga ttgcctggtc gaggtgcagc tcacggcctc ggccaaagtc gtagatgcgg
     2521 aatgtgtaat ctgacgtctg ttggacttcg taaaaaagca acctgccggt cgtgctgtgt
     2581 acgagcccgg gggacagtgt aacaacttca ccctgctgcg gtttaaattc atgaaaaaca
     2641 gagagaggct caggtaactc ttccagttgg cgcaaaactt cgggcgactg ttcacccttg
     2701 aggcccagat acatgcgtgc ctgcgggtca gcagaaagaa ttgtccatgc ctccatcttg
     2761 ccattgcctg ccaggccgag ggccttgacg tcgtcgtcag agggatggtt ctgtacggaa
     2821 atcggttctg cagttgagag taccttcagc ataaatggaa ctgcggcacc tgcacccatt
     2881 ttcttcgccc agaattctgc cagtggcatg tcggacgtac cgacttgaat tcgtacagga
     2941 aactggggca ggtcagaaaa aagtacatac tcgccaattt tttcggccga ggtattgccg
     3001 gtgagttttt ccaggtgccc gccgccccag ggtttgggga ctgcgtacgg aatgagttta
     3061 tagatgacct ggtctgcggc catgctggca gagcgggacg tcgaatcagg cctgcgatca
     3121 ttattcgtcc tggagtatct gcatgacaag caaggctacc tccgcaacaa gcaaaaggga
     3181 ttttcgatgc gataccgagc aagaaaccgg cagcatgact gctactgcga gctcaggact
     3241 ttcccgtttt taccgcgggc tactcgcagt agccctgctc tctctgccgc tggtcttttt
     3301 ctacaacctg gaactttttg gctggcttaa ctgccgcaaa gacgccacgg tactgaacta
     3361 cttcttcttg acgctgacct cgctcgccga tggtctctgg atcatgatgc tcgccacggt
     3421 cgtgcagagc gttgcgccgc ggaacttcaa ggcattcctc attgcgctgg tgctgggcaa
     3481 tgttttcctg cagagtggca agtatttctt cgatgccgac cggccgctgc gcctgctggg
     3541 tgaaagcgcc atctgcctgc tggggcagcc cctcacggtg cgcagttttc cctcggggca
     3601 ttccttttcc gtcggcctgc tgttcatgtt cctgcgcccg cgccattcat gggtgggagc
     3661 tttcgcgtta ctggcgattt cgctcctggc ggcagtctca cgcgcctacg tgggagtcca
     3721 ttttccgcgc gatatcatca gcggctttct gctggcggtg ctttctttcc tgggagctga
     3781 gatcctgtcg cgaaagatca ccttccgcga aacatcgctt accctgcgca agggactcgt
     3841 tgccttcctg ggcatgagta ttgccctgct ctatatcttt gcctaccacg aaaaaacccg
     3901 cgagctcgag gccgttttaa cacccgcggc gtgggtggtg gtgctttact ggtgtctcta
     3961 cgcggcgcgg ctgctggtgc tgagatatgg gtaggttgtg ttttctgcta taagccaatc
     4021 aagtttttgt tgacgtgatg tattgtttcc ggtaccttca gcagtgaagg cagctgagtt
     4081 tcatccagcg gcattgtctg agattaaatc ttattcaaag gaaataaaat ccaagttggg
     4141 agatttgata ctggatttgc agctcggtga gagtatcagt atgccagatt cgcggcctat
     4201 gccttcagtg ggacctggtg tgtctgaatt gagggttaag gactcgcaga atcaatatag
     4261 agctttctac tacttgaaag tagcggggaa agttataatc ttccatgcct ttgttaagaa
     4321 aaccgaaaag accctaaaac gagaaattga tatcggaaag aaacggttga aggatattct
     4381 ggaggcaatg aatgggtaaa ctaaacgtag tgacggcaaa aaatccaaaa gaactctttg
     4441 aagccatcgg cttaactgaa tgcgaggccg cagacgctat ttttcgggct aagttgaact
     4501 cggatattag tagcttattt ggcaaatcaa agttgaccca tgaggctttg gctaaaaaag
     4561 ttggtacctc acggacaaga attactgctt tgctcaacag aagccggtct gatattagca
     4621 cggattttat gcttaaggta ctggccgcac ttgggtacac ggtggagccg cgcctgaaga
     4681 aaatcgctta agacttattg cagggaacaa aatttacatg ctgcatgtcc tgcggtgccg
     4741 tggcttcgcc aaagacagag acttgaaaac tcgtcgcagg tggtttgacg acagtgccgc
     4801 cgctgcgtct cgtcgcgcgc aggaggttcg ggaagatttt ggcttgccgt taagactcaa
     4861 tcggtcaaaa ccttacgtaa attcaaagtc gtgtgcaatg ctttgaaaaa tcgtgacccg
     4921 aaaagtgaaa aggaaagtat tcccggctct tgttttgata ctattctact ctgtttcata
     4981 tggtgcagac aaccaggtgc cggaagactt tgaaccaatc aaagctggca atatcattaa
     5041 ggctaa
//
LOCUS       DAOQJF010000252         5018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9833_length_5018_cov_5.278662, whole genome shotgun sequence.
ACCESSION   DAOQJF010000252 DAOQJF010000000
VERSION     DAOQJF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9833_length_5018_cov_5.278662"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1035)
                     /locus_tag="PLF85_12450"
     CDS             complement(130..1035)
                     /locus_tag="PLF85_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE20527.1"
                     /translation="MYSFDAYPSPAGYFVAADGLQLPWFEYGDPKAAQTILLLNGFTC
                     NQFNIAKIVAGLSDNYRIVTFDYRGQGLAWQIEHENVSVDAVMADIDAFYRHLGSPRV
                     ILMGYSMGCQLAVEWNYRDNGNVQAMVLLLGIYGSIFDTFLGLGIFAPLLRITYDLFP
                     RFKEFYKAAWKLAHSLPYPLRVALGKGTLLNPDKVSEEELRPFLDQLAELDFEYLLHM
                     SHAIHHHTNEGQYHRIAAPCLIISGENDLFALPLHSERVHQAVPGSWYVSIPGGTHNA
                     VLENSEEIVEVVREFLQNKKSPGAS"
     gene            1111..2577
                     /locus_tag="PLF85_12455"
     CDS             1111..2577
                     /locus_tag="PLF85_12455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE20528.1"
                     /translation="MAQKVNTLLRRKMLLATVIFAAVTPILASALFITYRSFQQAKLD
                     AVDRAKSMLSHIAEKQDELVAQTMQSLRILTLLPEVRGAGERCSRFMSAFISINPVYD
                     NAGLVDAGGVIQCSGLPLKKDISVADREWFQLVAKTRAPQIGGLQFGKISGKPGIIAA
                     MPIVGSEGELKSVLYLAISVHWLEDVFAEYSLPDKSQITAIDANGLVLFQHPLKVDNE
                     ANMTGKPFPNEKVWNHIKASGEAIAPARVAEDGGSRHIYTFQRIEAEERVAMIIALRF
                     LESAIYRDAYHNAITLILGLCLSLVATFLIAYVAGNYLFLRPVEQEIEKLNDVAETDP
                     LTQILNRRGFTRLAENRLAAAEPTDHHAVLLMDIDHFKKINDEHGHATGDEVLRETVQ
                     RIRAVLRETEIFARVGGEEFAIFIPGVARETAILLAERIRVALDSLPFETAVGKLRVT
                     TSLGICHATNRITLPKFLDRADKALYKSKRTGRNKVSI"
     gene            complement(2617..3753)
                     /locus_tag="PLF85_12460"
     CDS             complement(2617..3753)
                     /locus_tag="PLF85_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNE20529.1"
                     /translation="MTVDAASLLTEMRRIHDAGKYSLVEVTRPWSKFNPTISGEFAHP
                     AALEWYLKREFTQLLQRGAQVRVTPARTRRDLFDPELIQTLSEDEWDLRKKKLFLFAA
                     ERIELSLNRLTHYTSTQAESFQRYILFTNYRMHVDAFLSLYPDCIKPSNPGAQMAAYH
                     HVLPDHAGITLINIGVGPANAKTITDHVAVLRPDLMIMVGHCGGLRNHQDIGDYVLAS
                     AYMRADRVMEEVLPNEIPVIPNFLINSYLRRELAERKIPYRMGTVYTTDNRNWEFNQR
                     RTIDAMKMSRSIAVDMESATIAANGYRYRIPHATLLCVSDKPLHGRPKLSNEAQAFYA
                     NSKERHLEVAIKTVEAIRSEYPQGLPNDDVRSFDEPLLGSSGND"
     gene            4062..>5018
                     /locus_tag="PLF85_12465"
     CDS             4062..>5018
                     /locus_tag="PLF85_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20530.1"
                     /translation="MKQFSFRFLILTFTGSLLLSATAVYPQVQFPEIPVAKDEEDTEA
                     GKKTEEKGKAEPKAEKQQVSEKGTPEAPKEPEKKTSTKDNQLSEAAIDRTGVWIPEFE
                     RQFAVRKTRPEFEFLWGFNMLQEFHSYNNADLRKLNSTNDFQIRTTDDQQGLALTRAK
                     FDTSFMFPKQRVGLEMSFGFDGVWGSFQMQGSGNPGTRIARANVFWEFFNLGGISIDT
                     VIGRQFYSVGGIQNDYMLRDVLDAIVFNIHWKDKIDVKVLGLDVYSGANSYGAGENDR
                     WNDEFQYFSRNTDGKLPGLNGDVSTYRTGLAVSGEPFMKTIFK"
BASE COUNT         1148 a         1324 c         1451 g         1095 t
ORIGIN      
        1 ggcgaagagc tcgacctgcc gccgggaaaa ttcattgtca ccgaggcgag cgaacgcgcg
       61 gtacgccgcg tcgaattcaa accccgcaga ggcgcacaaa agaaatagcg cagaacctct
      121 gcggcttctc taagatgcgc ccggcgattt cttattttgc aggaattcgc gtacgacttc
      181 cactatctct tctgaattct ctagcaccgc gttgtgcgtg ccgcccggaa tagaaacgta
      241 ccacgagcca ggaacagcct ggtgtacgcg ctcggagtgc aggggcaggg caaacagatc
      301 attttcgccg gaaataataa ggcacggtgc cgcgatgcgg tggtattgcc cttcgttcgt
      361 gtggtggtgt atggcatgcg acatgtgcag caggtattca aagtcgagct cagcaagctg
      421 gtcaagaaac ggcctgagct cttcttcgct gaccttgtcc gggttgagga gggtaccttt
      481 gcccagtgcg acgcgtaacg gatagggcag gctgtgcgca agtttccatg ccgccttgta
      541 gaattccttg aaacgcggaa acagatcata ggtgatacga agcagcgggg caaaaatacc
      601 caggccgaga aaagtgtcga agatgctgcc gtagatacct aagagcagta ccatcgcctg
      661 cacgttgccg ttatcgcggt aattccattc gaccgccagc tggcagccca tgctgtagcc
      721 catcagtatc acacgcgggc tgcccaggtg ccggtagaat gcatcgatgt cagccataac
      781 cgcgtcgacc gaaacgtttt cgtgttcaat ctgccaggcc aggccctgtc cacggtagtc
      841 aaatgtgacg atacggtaat tgtcagaaag cccagcgaca atcttcgcga tgttgaactg
      901 gttgcaggtg aagccgttca gcagcagtat tgtctgcgcg gccttcgggt cgccgtattc
      961 aaaccacggc agttgcaaac catccgccgc gacgaagtag ccggccggcg aagggtaagc
     1021 gtcaaaactg tacatccccg cgagtcgcgt gcggcgcgag cgcgtcaaga aatacttgaa
     1081 gccaagcgtc gcgaaaaatt tatgaacgcc ttggcacaga aggtcaacac actgcttcgc
     1141 cgcaagatgc tgctggccac ggtgattttt gccgcggtta cgcccatact cgcttcggcg
     1201 ctctttatta cctatcgctc gttccagcag gcaaaactcg atgcggtcga ccgcgcgaaa
     1261 tcgatgctct cgcacattgc cgaaaagcag gacgaactcg tcgcgcagac catgcagtcg
     1321 ctgcgcatcc tgacgctgct gcccgaagtg cgaggcgccg gcgagcgctg ttcgcgtttc
     1381 atgtcggctt ttatttctat caacccggtt tacgacaatg cggggcttgt cgatgcaggg
     1441 ggagtcatcc agtgcagcgg attgcccctg aaaaaagata tcagcgtcgc cgaccgcgaa
     1501 tggttccagc tggtggccaa aacccgcgcc ccgcagatcg gcggcctgca gttcggcaag
     1561 attagcggta aacccggtat catcgcggcg atgccaattg ttggcagcga gggggagctg
     1621 aaatcggtac tgtacctggc cattagtgtt cactggctcg aagatgtctt tgcggaatac
     1681 tcactgccgg ataagtcgca gattacggcg attgatgcca atggactcgt gctttttcag
     1741 catccgctca aggttgacaa cgaagccaac atgacgggaa aaccctttcc caacgaaaaa
     1801 gtgtggaatc acatcaaggc ctcgggcgaa gcgattgcgc cggcgcgggt tgccgaagat
     1861 ggcggtagca ggcatattta caccttccag cgtatcgagg ctgaagagcg ggtggcgatg
     1921 atcattgccc tgcgttttct tgaatcggca atctaccgcg atgcttacca caacgccatc
     1981 accctgatac tgggtctctg cctctcgttg gtggcaacct ttctcatcgc ctacgttgcc
     2041 ggcaattacc tcttcttaag accggtagag caagagatcg aaaaactcaa tgacgtggcc
     2101 gaaaccgacc cgctgacgca gatattaaac cgccgtggtt ttacccgcct tgccgagaac
     2161 cgcctggcag cggccgaacc cacagaccat cacgccgtgc tgctgatgga catcgatcac
     2221 ttcaaaaaaa tcaacgacga acacggccat gcaacaggcg acgaagtgtt gcgcgagact
     2281 gtccagcgca tccgcgccgt cctgcgcgag acggaaattt tcgcgcgggt cgggggcgaa
     2341 gagtttgcta ttttcattcc gggtgttgcg cgcgaaacgg caattctgct ggcagagcgc
     2401 atacgcgtgg cgctcgatag ccttccgttt gaaacggcgg tgggcaagct gagggtaacc
     2461 accagcctcg gtatctgcca cgcgaccaac cggattacgc tgccgaagtt tctcgaccgg
     2521 gctgacaaag cgctctacaa gagcaagcgc acgggacgaa acaaggtgag tatttaatgc
     2581 cctacctccc ccgaaggggg aggtagggca gggatatcag tcgtttcctg aactgcccaa
     2641 taatggttca tcaaaagagc gtacgtcgtc gttcggcagg ccctgagggt attcgctgcg
     2701 tattgcttcg acggtcttaa tcgccacctc gaggtgacgc tctttgctgt tggcgtaaaa
     2761 ggcctgcgct tcgttggaga gttttggccg gccgtgcaac ggtttatcgc tcacgcacag
     2821 cagcgttgcg tgcggaatgc ggtagcggta gccgtttgcc gcaatcgttg ccgattccat
     2881 atcgaccgcg atcgagcgtg acatcttcat cgcgtcaatg gtgcggcgtt ggttgaactc
     2941 ccagttgcgg ttgtcggtcg tgtagaccgt gcccatgcgg tagggtattt tgcgttcggc
     3001 aagttcgcgg cgcaggtacg aattgatcag gaagttggga atcaccggaa tctcgttggg
     3061 tagtacttct tccatcacac ggtcggcgcg catgtacgcc gacgcaagaa catagtcacc
     3121 gatatcctgg tggttcctga gtccgccgca gtgtccgacc ataatcatca ggtcggggcg
     3181 cagaacagca acatggtcag taatggtttt ggcgttcgcc gggccgacgc cgatattgat
     3241 cagcgtgatg cccgcatggt cgggcagcac gtggtggtag gccgccatct gcgcaccggg
     3301 gtttgagggt ttgatacaat cagggtagag tgaaagaaat gcatcgacgt gcatacggta
     3361 gttggtgaac agaatatagc gttggaaact ctcggcctgc gtcgaggtgt agtgggtaag
     3421 gcggttgagc gagagctcga tgcgctcggc ggcaaacagg aacagctttt tcttgcgcag
     3481 atcccattca tcttcgctga gtgtctgaat cagttcagga tcaaaaagat cgcgccgcgt
     3541 gcgcgcggga gttaccctga cctgtgctcc acgctgcaga agctgcgtga actcgcgctt
     3601 caggtaccac tcaagcgcag ccgggtgggc aaattcaccg ctgattgttg ggttaaattt
     3661 tgaccaggga cgtgttacct cgaccagcga atatttgccg gcatcgtgta tgcggcgcat
     3721 ctcggtcagc aggcttgcgg catcgacggt catagttcag ggtgcgtgag gcgaggatgc
     3781 gtcaagccgg aaaaccctgc atactcgtcc tttccaatca gtggcatatt tcttgctgat
     3841 ttgggtgcgc aggggatcca tgggggtaac tcaactatga atgcgtttga agcagtacgg
     3901 tagctgaaaa ttttggccct gaatgagtgc ggcagcgtat ctatgttcac tgtggattgc
     3961 ctttacggta cttcgtcgat taacgaaatg tcctagtgcg cctgggggtg ccgcaatggc
     4021 gctgtttgca ttttcctaaa actaaaaaag tgagtaaaac tatgaagcaa ttttctttcc
     4081 gtttcctgat tcttacattc accggtagcc tgctgctctc ggctacagcc gtttatccac
     4141 aggtgcagtt tcccgaaatt ccggtggcaa aagatgaaga ggatactgaa gcgggtaaga
     4201 agaccgaaga aaaaggtaag gctgagccga aagctgagaa gcagcaggta agcgaaaaag
     4261 gaacacccga agcacctaaa gagcccgaga aaaaaacgtc tacaaaagat aaccagcttt
     4321 cagaggcagc catcgaccgt acgggtgtgt ggatacccga gtttgagagg cagttcgccg
     4381 tcagaaagac gcgccctgaa tttgaattcc tctggggctt caatatgctg caggagttcc
     4441 actcatacaa caatgcagac ctgcgcaaac tcaacagtac gaatgatttc cagatacgca
     4501 caacggacga ccagcagggc ctggcgctca cgcgcgcgaa gtttgatact tcgtttatgt
     4561 ttcccaagca gcgcgtcggg ctcgagatgt cattcggttt tgacggtgtc tggggtagtt
     4621 tccagatgca gggttcgggc aaccccggta cgcgtattgc gcgtgccaac gtcttctggg
     4681 agttcttcaa cctcggtgga atttctatcg atacggtcat cggccgccag ttctacagcg
     4741 tcggcggtat ccagaacgac tacatgctgc gcgacgtact ggacgcgatt gttttcaaca
     4801 tccactggaa ggataaaatc gacgtaaaag ttttgggact cgatgtgtac tcgggcgcca
     4861 acagctatgg cgcaggggag aacgatcgct ggaacgacga attccagtat ttctcgcgca
     4921 atacagacgg caagctgcca ggtctcaacg gcgacgtttc gacttaccgc acggggcttg
     4981 cggtcagcgg cgagccgttc atgaagacta tattcaag
//
LOCUS       DAOQJF010000253         5000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9879_length_5000_cov_4.741355, whole genome shotgun sequence.
ACCESSION   DAOQJF010000253 DAOQJF010000000
VERSION     DAOQJF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9879_length_5000_cov_4.741355"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PLF85_12470"
     CDS             complement(<1..1012)
                     /locus_tag="PLF85_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNE20531.1"
                     /translation="MLYISMFAGLLASPLAAEKLSAGEFSGTGSYEYLFFPAGRQAEA
                     QELTRRIAELNEEEKRLAADLARAEAEIAAIEGREEKKKSTRIDWSDIENATGYSVKL
                     YDADKKLLSTHSTEENFIVLELVPADYHFQVAAVTKYKTGAYSRMTRLKVSKGKASEQ
                     KLKAEENAEAVRERLRLAKKFRAGHAETLGQLALSRETANAVPADLKVPEAATSYLVA
                     EQGDAPRYSGVIALPGRQVAIAGRAASSEPTSNFLWGAGLLAGIQDTRLDYFRVTLGA
                     EAFLRYDRPFLRFFYPQVKFMSGVSPAKDQVFDSMVHSLLYAGAYYPIEIGRGFRLLG
                     SIS"
     gene            complement(1105..1626)
                     /locus_tag="PLF85_12475"
     CDS             complement(1105..1626)
                     /locus_tag="PLF85_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20532.1"
                     /translation="MKKRNLIAGLLGVLLLFGAGLEAQKPKVTKNKKGGYSIKHPAKI
                     APMVSEENMYIQGESGGTIQISVSPMYSPITACEQLTQIEALYEGKRTNAYPEEARRP
                     PAKVQKAVGLKDGCRARYEIADESGSRIEEMAVFTDPKKKKIYQLSVGYEAGLNEQSV
                     SELMQIMSSFTVK"
     gene            complement(1711..2337)
                     /locus_tag="PLF85_12480"
     CDS             complement(1711..2337)
                     /locus_tag="PLF85_12480"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE20533.1"
                     /translation="MRRKSSLCAFLCACSFFAVTACNNYDLLQKLENPGQSSVGPGPR
                     NLRIFVSNAFNNGNLGGAAGADIICLSDTANPMGADNGSWKALIVGGGRRACSTPYCS
                     GGAAENIDWALKPNTNYVRPNGLVIGNTDPAGLLVFPLNSAISDLTTTFAWTGLAGDW
                     MTGSTCTDWGNGTSGSGDRGGVDKNDPQAINIGIDTCGTSQRLYCVEQ"
     gene            2477..3739
                     /locus_tag="PLF85_12485"
     CDS             2477..3739
                     /locus_tag="PLF85_12485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE20534.1"
                     /translation="MKSPLFLYLFAATVIAAEPLKMSFGEAMQRLVAQNRDLQAARQN
                     LETARLNYRGAYAGFLPQVTAGMNYTQGNSATTAQLAGVNTTYELYSASLGVNQNIFS
                     GLADYYKIKQARANTELSEATYTQTAARVYYELKAAWSQLQLARAQHKLALETRARRL
                     ENVRMIRLRFNSGNENKGSLLLAESYLAQAERDVNQALRQKLTAETELRRVLNLPDEQ
                     EVLPEGSIASKAPPEAQNLAELSKQTPEYRQALAQELNAEATADAAVSPFFPTLNASG
                     SLFRQGPTFFPERDRWSVTVGVSYPLFSGGKDYYALKANSSAHEASKRNRESVELART
                     ARLQQALQTWRDALESAKVSESLLGASTVRAQIARVKYNNGLLSFDQWDIIENDLINR
                     QKGVLQTRYDLEIAQAAWEQLTGEGPAL"
     gene            3739..4725
                     /locus_tag="PLF85_12490"
     CDS             3739..4725
                     /locus_tag="PLF85_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE20535.1"
                     /translation="MSKLRSKPLWVILAAAALGAALYAFWPSGKKSEAPSYSEIKPQR
                     GEIRVTVTSTGTVQPEGRIEMKPPVPGRMESVLVREGQKVRRGQIIAWMSSTERAALL
                     DSARAVSAEEFKRFQEYYKATPIVAPVSGTIILRNVEPGQTVASADVLLVVSDRLQVV
                     AKVDETDIARVERGVPAEILLDAYPQQPIEARVSAIAFEAKTVNNVTTYDVEVAPKAQ
                     PAFMRSGMTANVTFILKKKEDVLTLPLAAIQFRKDSSFVLVKGSKGKPEEVLVAIGDS
                     DGSNTEITSGLDEAQTVLVPKMQVNTGKQGTNPFMPARSGSSGKGKSSNRTH"
     gene            4732..>5000
                     /locus_tag="PLF85_12495"
     CDS             4732..>5000
                     /locus_tag="PLF85_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE20536.1"
                     /translation="MIEVRNLGRKYVMGGNTVHALKNVNLDIAPDEFVAIMGRSGSGK
                     STLMHVLGLLDQPTSGSYRLFGKEVAHMEEDALAVYRRATLGFIF"
BASE COUNT         1119 a         1438 c         1362 g         1081 t
ORIGIN      
        1 tagatataga acctaacaaa cgaaaaccgc ggccgatttc aatggggtag tacgcgcctg
       61 catagagcag cgaatgcacc attgaatcga acacctggtc tttggcgggc gaaacgccgc
      121 tcatgaattt cacctgcggg taaaagaaac gcaggaatgg tctatcgtac cggagaaatg
      181 cttcggcgcc cagcgtgacg cggaagtagt cgagccgggt atcctgtatg ccggcaagaa
      241 gccctgcccc ccacagaaaa ttgctcgttg gttcgctgct ggctgccctg cctgcaatag
      301 cgacctgccg accgggtagc gcgattaccc cagagtagcg aggcgcatcg ccctgctcag
      361 caaccagata actcgtcgca gcttcaggaa ccttgagatc tgccggcacc gcattcgccg
      421 tttcacggct gagcgccagc tgccctaatg tttcggcatg gccggcccgg aattttttcg
      481 cgaggcgcag tctctcgcgc accgcttcgg cattctcttc ggcctttagt ttctgctcgc
      541 tggctttacc cttcgaaacc ttgaggcgtg tcatgcgcga atacgcaccg gtcttgtact
      601 tcgtgaccgc cgcaacctgg aagtggtaat ctgccggaac cagttcaagc acgatgaagt
      661 tttcttcggt gctgtgtgta ctcagcagct tcttgtcagc atcgtagagt ttgacgctgt
      721 aaccggtggc gttttcaata tccgaccagt cgatgcgcgt ggactttttc ttttcttcgc
      781 gaccctcgat ggcggcaatt tcagcctcag ccctggcgag gtcagcagcc agacgctttt
      841 cttcttcgtt aagctctgcg atacgccggg ttagctcctg cgcttctgcc tgccggcccg
      901 caggaaagaa caggtattca taactacccg taccggaaaa ttcgccggcg gaaagtttct
      961 cggccgcaag aggcgacgcc agtaaaccgg caaacataga aatgtaaagc atcctgcgca
     1021 ctgtgttagc gatcactgca gtgcgcagga gacgtcaagc attagcgcgg tagcacctgc
     1081 tcaacaatga taccatgccc gtttttactt aacggtaaac gagctcatga tctgcatcag
     1141 ttcggacacc gactgctcat tgagtcccgc ttcatagccc acagaaagct ggtagatttt
     1201 ctttttcttc ggatctgtga aaactgccat ttcttctatg cgcgaaccac tttcgtcggc
     1261 gatttcataa cgcgcacggc aaccgtcttt cagccctact gccttctgca ctttggcagg
     1321 cgggcgacgc gcttcttcgg ggtatgcatt ggttcgcttg ccttcgtaaa gcgcctcgat
     1381 ctgggtgagt tgctcgcatg cggtaatggg cgaatacatg ggggaaacag aaatctggat
     1441 ggtgccaccc gattcgccct ggatatacat attttcttcg ctcaccattg gtgcgatttt
     1501 ggccgggtgc ttgatagagt aaccgccttt cttgttttta gtgactttcg gcttctgtgc
     1561 ttcgagccct gcaccaaata ggagcaatac gcccaataag cctgctatga ggtttctttt
     1621 cttcattctg ttctcctgat ctattttttt taaaatgaat taatccagtg tcagctgggg
     1681 ttacagcgat ttgcagaggt acatcgatgc ctattgctcg acgcagtaaa gtcgctgtga
     1741 agtgccgcat gtatcgattc cgatattgat cgcctgagga tcgttcttgt caaccccgcc
     1801 cctgtcgcca ctaccgctgg tgccatttcc ccaatccgta caggtgctac ctgtcatcca
     1861 atctcctgcc agacctgtcc acgcaaatgt cgtcgtcagg tctgagatag cgctatttaa
     1921 cggaaatacc aggagaccag cagggtctgt gttgcctatc actaagccgt tgggtcgaac
     1981 gtaattcgtg ttgggcttca atgcccaatc gatgttctcg gcagctccgc cactgcagta
     2041 cggcgtcgag cacgcccgcc gcccgccgcc gacaatcaaa gctttccagc taccattgtc
     2101 ggcgcccatt ggatttgccg tatccgacag acagatgata tcagcaccgg cagcaccccc
     2161 gagattgcca ttgttgaatg cgttgctgac aaaaatgcgc agattgcgcg gaccaggacc
     2221 gacagatgat tgtcccgggt tttccagctt ttgcagcagg tcataattat tgcacgccgt
     2281 gaccgcaaaa aatgaacatg cacacagaaa cgcacatagc gaggattttc gtctcattcg
     2341 cccgacgttc agcacgctct acagagggtc aagctgattg tagggaaatc atcccgttac
     2401 tacaattcgg ctgcaggacg ccgataatgt aacacaggaa ccaatcgccc ccttccgggg
     2461 tcactttaca gaacttatga aatccccctt gtttctttat ctatttgctg ctactgtcat
     2521 agcggcagaa cccctgaaga tgagttttgg cgaggccatg cagcggctcg tcgcacagaa
     2581 ccgcgacctg caggctgcgc gacagaatct cgagaccgcg cggctcaatt accggggtgc
     2641 gtatgcaggt tttttaccgc aggtcaccgc cggcatgaac tacacgcagg ggaactcggc
     2701 gacgaccgcg cagcttgcag gcgtcaacac gacgtatgag ctctactccg cttcgctggg
     2761 tgtgaaccag aatatttttt cgggactcgc cgattattac aagatcaagc aggcgcgcgc
     2821 caataccgaa ctttctgaag caacctatac gcagaccgcg gcgcgtgtct actatgaact
     2881 caaggcagca tggtcacaac tgcaacttgc gcgtgcgcaa cacaagctcg cgctcgaaac
     2941 acgcgcgcgc cggctcgaga acgtgcgcat gattcgcctg cgcttcaaca gcggtaacga
     3001 gaacaagggt tcgttgcttt tggccgaatc ttacctggcg caggctgagc gcgacgtcaa
     3061 ccaggcgctg cggcaaaagc tcacggctga gacggagctc aggcgcgttc tgaacctgcc
     3121 cgacgagcag gaagtattgc ccgagggatc gatagcgagc aaggcaccgc ctgaagcgca
     3181 gaacctcgca gaactttcga aacagacacc tgaataccgc caggcgctcg cgcaggagct
     3241 gaatgcggaa gcgacagccg atgctgcagt ctcgcctttc tttccgacgc tcaatgcttc
     3301 gggctcgctc ttcaggcagg gaccgacctt ttttcccgaa cgcgaccgct ggtcggtaac
     3361 cgtcggggtg agctatccgc tcttcagcgg tggcaaagac tactacgcgc tgaaggccaa
     3421 cagcagcgcg cacgaagcct cgaagcgtaa ccgcgaatcg gttgaactgg cgcgcacggc
     3481 ccggctgcag caggcgttgc agacctggcg cgatgcgctc gaaagtgcga aggtcagcga
     3541 atctctgctg ggtgccagta cggtgcgcgc gcagatcgcc cgcgtaaaat acaacaacgg
     3601 cctgctgtcg ttcgaccagt gggatattat tgaaaatgat cttattaacc gtcaaaaagg
     3661 tgttttgcag acccgctacg atctggagat tgcacaggct gcgtgggaac aactcaccgg
     3721 cgaaggacct gccctgtgat gtcaaaactg cgctccaaac cattatgggt tatcctggct
     3781 gcagcagctc tcggcgcggc actctacgcc ttctggccca gtggcaagaa gagtgaagct
     3841 cccagctatt cagagatcaa accgcagcga ggcgaaattc gtgtcacagt gaccagtacc
     3901 ggcacagtcc agcccgaggg ccgcatcgag atgaaaccgc cggtgccggg ccgcatggag
     3961 agtgtgctgg tgcgcgaagg gcaaaaagta cggcggggcc agattatcgc ctggatgagt
     4021 tcgaccgaac gcgcggcatt gctcgattcg gcgcgcgcgg tgagcgctga agaattcaaa
     4081 cgtttccagg aatactacaa ggcaacgccg attgttgcgc ctgtctcggg cacgatcatt
     4141 ctgcgcaacg ttgagcccgg gcagacggtc gcttcagccg atgtactgct cgtcgtctct
     4201 gaccgcctgc aggtcgtcgc caaagtcgat gagaccgaca tcgcgcgcgt cgaacgcggc
     4261 gtgccggcag aaattctgct cgatgcatac ccgcagcagc cgatcgaagc gcgcgtcagc
     4321 gccattgcgt ttgaagcgaa gaccgtgaac aacgtcacca cctacgatgt cgaggtagct
     4381 cccaaagcgc agccggcctt catgcgcagc ggcatgacgg caaacgtcac attcattctg
     4441 aaaaagaaag aagatgtact gaccctaccg ctcgcagcca tacagttcag gaaagattca
     4501 tcgtttgtgc tggtgaaggg gtcaaaaggc aaacccgaag aagtgcttgt ggccatcggc
     4561 gattcggatg gcagcaacac cgaaatcacc tcggggctcg acgaggcgca gacagtgctc
     4621 gtgcccaaaa tgcaggtcaa taccggcaag cagggtacca acccgttcat gccggcccgc
     4681 agcgggagct cgggaaaggg taaatcttcc aaccgtacgc attaaccggg catgattgaa
     4741 gtccgcaact taggccgcaa gtatgtcatg ggcggcaaca ccgtccatgc gctgaagaat
     4801 gtaaatctcg acattgcccc cgacgagttc gtcgcgatca tgggccgttc gggttcgggt
     4861 aaatcgactc taatgcacgt tctggggttg ctagaccagc ccaccagcgg tagctaccgc
     4921 ctgttcggca aagaagtcgc gcacatggaa gaagatgcgc tggcggtcta ccgtcgcgcg
     4981 acgctcggct tcatcttcca
//
LOCUS       DAOQJF010000254         4995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9896_length_4995_cov_6.198785, whole genome shotgun sequence.
ACCESSION   DAOQJF010000254 DAOQJF010000000
VERSION     DAOQJF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9896_length_4995_cov_6.198785"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLF85_12500"
     CDS             <1..708
                     /locus_tag="PLF85_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20537.1"
                     /translation="NEKGRITPVRFTERYTHRYRFARELAPREIIADTVTSLPSEDVG
                     YDNAIKVAQESSTVTGPTGPLEIATEETEVAPTTGPQKKPQRPKPETIVGKYIVAVYP
                     VNMEVIAGKVKNYSLKEKLRGNFLIEVNRGGAGRLINASKMTASPVQGDRELESAYLK
                     KLQQSLGADLIVFNKLYTDGNTGQLKLVTIYYKRGDNGISAQTEVEGVDSQELKFVRE
                     ATEKFTTEHKALLGELK"
     gene            710..1216
                     /locus_tag="PLF85_12505"
     CDS             710..1216
                     /locus_tag="PLF85_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20538.1"
                     /translation="MWNILARLLVALLLLGSAGALSAVEFGGGKFEYMNSKETSFMGF
                     GLFTSNKRFDAEVEGFVAPAVFTGNTDNAGYLGSNYRLFFVAFSGYFHFLRTDKMSLF
                     VGGGIMPWLPKTYAYHFAAGMDFFFSEEWRIFYVFRYMDNNAENYRYPTGTSVAVGFK
                     YAFRMLNI"
     gene            1213..1812
                     /locus_tag="PLF85_12510"
     CDS             1213..1812
                     /locus_tag="PLF85_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20539.1"
                     /translation="MKRTSIFALIAALSLLAANCRSRGPQIKAPRENLPATEFHHEPA
                     KVIPWFYGNKSSRGLDRQADAAFFDQVGGGVGLLVNSRLDSQWDGHKFFYYYLDFDFS
                     PQFNPGKTYPGDKQWIFSSYPGIMFRTYTPFYLKMHFGFGMNVRYNQQHYDRWGVYGM
                     MGAELWGFTSTVVFIGHPGQLNWETEYRAGYMWAPVEWK"
     gene            1814..2593
                     /locus_tag="PLF85_12515"
     CDS             1814..2593
                     /locus_tag="PLF85_12515"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNE20540.1"
                     /translation="MSTIQRRVTALILVLPAFFAGSLSAGEYTNSFKIKIGEEDAVGE
                     SLKEDPVEAFNNAVEAAMKTGQSLGEAELIGGSRTKDFVLTDSWVKKEAHLQVTDLQV
                     KKYRFWKPAGEPVRVSMNVEMQVDYVDVPKFTEDYEKTVTGATYRSMAIPGWGQIYNR
                     QYTTSILYGAAFWTFYVMFIQGVKNANSSGDISTAALNFQLPALILWSFNVSEAATSR
                     IMGRQGLENLRQAYRLDARHEYVPMTERGWKLDFVFYEFSF"
     gene            2613..3464
                     /locus_tag="PLF85_12520"
     CDS             2613..3464
                     /locus_tag="PLF85_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20541.1"
                     /translation="MKPVLILFAAALLTGGIAAKPSSVDLGLDKEYFSSQKKVRVRDA
                     IGKGRSREIAIQNARIDAHRKALERSVSSFRHMLVETKDFVVTNEQLIADVNAKVIEE
                     ARCNEPKWDHGMNFDPFHVAVMDCMVTVSFFDLDYFTTEIMKTAEGACIRSIVLSGWG
                     QFYNKNYVTSAAMALITYGSIGYGYSREVKVAGARDEYKNAATPAEAEQKYKQMRDHQ
                     EVARTMYVIGALTWAYSIWEAFEDRERADDVLYQVHQKYFKGKLNYEPQISFFQRFMM
                     QNTRPKW"
     gene            3458..3946
                     /locus_tag="PLF85_12525"
     CDS             3458..3946
                     /locus_tag="PLF85_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20542.1"
                     /translation="MVRLPLLVTLIASLAGCAITEVPLGHDNPLDRIYDSGKFRMVLT
                     AEAKSASLTQLTWSNVFHSEDGELTDAELKLNNSATILRSAVAPIAADLTAVKNQATL
                     SGAFATVGECSVKVTGTGYAGNCDVSSVPKGYFIIQFNYRYTGKAGTVAGILYSNFVV
                     VQ"
     gene            3943..>4995
                     /locus_tag="PLF85_12530"
     CDS             3943..>4995
                     /locus_tag="PLF85_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20543.1"
                     /translation="MKELLKLRIFSLLLCAGFLGQVWGQKKAAPPPKRAARPAAAKPA
                     APPALPAKTAAKPAAGVEERELAVPKPTAAKLPEIERQYEDINKNPTRGTFTFSMSAG
                     LYNFNNKIENVSKNTTREYSLQFDATTMLGMELAWRLGKDKENDHDTGNGFHYYRSNR
                     GIFTDFELGFKTQRTEDKPSRFNVRQTVNGAATDLPVDLGYDYTAAAFLANTAPDAQR
                     LRQAGISPEARQSVGKTAFEGTMVYANTYYHFTPFNMLMNWGSAFRWFDSSIGPSLRV
                     WYYRDYSDPVRVSTRNDDWTRATFMIVYRQYIQFHPLVRLRTHFYFPALSFFGEMAKS
                     PYFNEKEYILNTALEFY"
BASE COUNT         1216 a         1307 c         1372 g         1100 t
ORIGIN      
        1 aatgaaaagg gccgtatcac gccggtgcgc tttaccgagc gttatacgca ccgctaccgt
       61 tttgcgcgcg aactggcacc gcgtgaaatt atcgccgata cggtgacgag cctgccatct
      121 gaagatgtcg gttacgacaa cgcgatcaag gtcgcgcagg aatcaagcac cgtaacaggg
      181 ccaaccgggc cgcttgagat tgccactgaa gaaacagagg ttgctcccac aaccgggcca
      241 cagaagaaac cgcagcggcc aaagcctgag acgattgtag gcaaatatat tgtcgcggtc
      301 tacccggtga atatggaagt gattgccggt aaggtgaaga actattcgct gaaagaaaag
      361 ctgcggggca acttcctgat cgaggtcaac cgcggcggtg cgggccgcct gatcaacgcc
      421 agcaagatga ccgcatcgcc ggtacagggc gaccgcgaac tcgaaagcgc atacctgaaa
      481 aagctgcagc agagcctggg ggccgacctg attgttttta acaagctcta taccgacggc
      541 aacaccgggc agctcaagct cgtgacgatc tactacaagc gcggcgataa cggtatttcg
      601 gcgcagacag aagttgaagg tgtcgattcg caggaactca aattcgtgcg cgaggcgacg
      661 gaaaaattca cgacagagca caaagccctg ctcggggagc tgaaataaaa tgtggaatat
      721 cttagccagg ttactcgtcg cgctgctgct attgggttca gcaggcgcgc tttctgctgt
      781 ggaatttggc ggtggcaagt tcgagtacat gaactcgaaa gaaaccagtt tcatgggttt
      841 cggcctcttc acttcaaaca agcggttcga cgcagaagtt gagggctttg ttgccccggc
      901 ggtatttact ggtaacacag ataatgcagg ctacctcggc agcaactacc ggctgttctt
      961 cgtggcattt tccgggtact tccatttctt gcgtaccgac aagatgagtc ttttcgtggg
     1021 cggcggcatt atgccatggc tgccgaagac ctatgcgtac cacttcgctg cgggcatgga
     1081 cttcttcttt tctgaagaat ggcgcatctt ctatgtgttt cgttatatgg ataacaatgc
     1141 tgaaaattat cgctacccga caggcacatc ggtagcggta ggcttcaaat acgctttccg
     1201 gatgttgaac atatgaaaag aaccagcatc ttcgccctga tagcagcgct ttcgctgctg
     1261 gcagcaaact gccgttcacg cggcccgcag atcaaggcgc cacgtgaaaa cctgccagcg
     1321 accgagtttc accatgaacc ggcaaaggtc ataccgtggt tttatggtaa taagtcttca
     1381 cgcggcctcg accgccaggc agacgccgcg ttctttgacc aggtcggtgg cggggtaggc
     1441 ctgctcgtga attcacgcct cgattcgcag tgggatggcc acaagttctt ctactattac
     1501 cttgattttg atttctcgcc ccagtttaac cccggcaaga cctacccagg tgataagcag
     1561 tggatattct ccagttaccc gggtataatg ttccgcacct acactccctt ttacctcaaa
     1621 atgcactttg gttttggcat gaacgtgcgt tacaaccagc agcactatga ccgctgggga
     1681 gtttatggca tgatgggtgc agaactctgg ggcttcacct caacagtggt ctttattggt
     1741 catcccgggc agctgaactg ggaaacagaa taccgtgccg gttacatgtg ggcaccagtg
     1801 gagtggaaat agtatgagta cgatacaacg ccgtgtgaca gccctgattc tggtgctgcc
     1861 cgcatttttc gccggcagcc tcagcgcagg cgaatacacc aacagtttta aaatcaagat
     1921 cggtgaagaa gatgcggtcg gcgaaagcct gaaagaagat cccgttgagg cgtttaacaa
     1981 tgccgtcgaa gcggcgatga agacggggca gagccttggc gaggccgaac tgatcggcgg
     2041 cagccggaca aaagatttcg ttctgactga ttcatgggtg aagaaagaag cgcacctgca
     2101 ggtcaccgat ctgcaggtca agaagtaccg tttctggaaa ccggcgggcg agcccgtgcg
     2161 cgtgagcatg aacgtcgaga tgcaggtcga ctacgttgac gtgcccaaat ttaccgaaga
     2221 ctacgagaag acggtaaccg gcgccacgta tcgctcgatg gcgataccag gctggggcca
     2281 gatctacaac cggcagtata ctaccagtat tctctatggc gccgcattct ggacattcta
     2341 cgtgatgttt atccagggtg tgaagaatgc gaacagcagt ggcgatatct cgactgcagc
     2401 tctgaacttc cagctgccgg cgcttatcct gtggagcttc aacgtttctg aagcggcgac
     2461 ctcgcgcatc atggggcgcc agggcctcga gaacctgagg caggcctacc ggctcgatgc
     2521 aagacacgaa tatgtaccaa tgaccgaacg cggctggaaa cttgactttg tattctatga
     2581 gtttagtttt tgagggcatg atggctaaga atatgaaacc tgttctcata ctattcgcag
     2641 cagctctgct gaccggtggc atcgccgcaa agccttcgag cgtcgatctg gggctcgata
     2701 aagaatactt ttcgtcgcaa aaaaaggtca gggtacgcga tgcgattggc aagggtcgct
     2761 cgcgggaaat cgctatccag aatgcccgta tcgacgctca ccgcaaggcg cttgaacgct
     2821 cggtgtcgag cttcaggcac atgctggtcg agacgaaaga cttcgtcgtg accaacgagc
     2881 agctgatcgc tgacgtgaat gccaaggtca tagaagaggc gcgctgcaac gaacccaaat
     2941 gggaccatgg catgaatttt gacccgttcc acgtggcggt catggattgt atggtgactg
     3001 tgagcttttt tgacctggac tatttcacga ccgagatcat gaaaactgcc gagggtgcat
     3061 gtatccgttc gatcgtgtta tctggctggg gccagttcta caacaagaat tatgtgacga
     3121 gtgccgcgat ggcgctgatc acctacggct caatcggtta tggctattcg cgcgaagtca
     3181 aggtagccgg cgcacgcgac gaatataaga acgctgcgac accggcggaa gccgagcaga
     3241 aatacaagca gatgcgcgac caccaggaag ttgcccgtac tatgtacgtg atcggggcgc
     3301 tgacctgggc ctactctatc tgggaggcgt ttgaagaccg tgagcgggcc gacgatgtac
     3361 tctaccaggt gcaccagaaa tacttcaagg gtaagctcaa ctacgaacca cagatttctt
     3421 tcttccagcg cttcatgatg caaaacacga ggccaaaatg gtgagactgc cgcttctcgt
     3481 aacgctcatt gcgtcgcttg cgggctgcgc gattaccgaa gtgccactgg ggcacgacaa
     3541 tccacttgac cgtatttatg acagtggcaa gttccgtatg gtactgacag ccgaagcaaa
     3601 atcggctagc ctgacccagc ttacctggag taatgttttt cacagtgaag acggcgaact
     3661 gacggacgca gagctcaagc tcaacaacag tgctaccatc ctgcggagcg cggtggcgcc
     3721 cattgctgct gacctgactg cggtaaagaa ccaggcaaca ctttcgggag cctttgccac
     3781 tgtcggggaa tgctctgtga aggtgacagg tactggctac gccggtaact gcgatgttag
     3841 cagtgtaccc aagggatact ttattatcca gttcaattac cgctataccg gcaaagccgg
     3901 cacggttgca ggaattttat attccaattt tgtggtggtc caatgaaaga gctactgaaa
     3961 ctcagaatat tttccctgtt gctctgcgca ggcttcctgg gtcaggtctg ggggcagaag
     4021 aaggctgcac caccaccgaa gcgcgcagcg cgccccgctg cggccaagcc tgcagcacct
     4081 ccagcgctgc cggcaaaaac cgctgccaaa ccggctgccg gggtcgaaga gcgggagctc
     4141 gctgtgccaa aacctacagc tgcaaagtta cccgaaattg agcgccaata tgaagatatc
     4201 aataagaacc ccactcgcgg tacttttact ttcagtatgt ccgccggtct ctataacttc
     4261 aacaacaaga tcgagaacgt ctcgaagaac accacgcgcg agtattcgct gcaatttgac
     4321 gcaacgacaa tgctgggtat ggagctggcc tggcgtctgg gcaaagacaa agaaaacgat
     4381 catgatacag gaaacggttt ccattactac cgctcaaacc ggggaatttt cactgatttc
     4441 gaactgggtt tcaagaccca gcgaaccgaa gacaagccat cgcgctttaa tgtaaggcag
     4501 accgttaatg gagctgcgac cgatctgccg gtggatctgg gttatgacta tacagccgcc
     4561 gcttttcttg ccaacacggc acccgacgcg cagcgcctgc gccaggctgg tatttcaccg
     4621 gaggcaaggc agtctgtggg taagacggcg ttcgagggta cgatggtcta cgccaatacc
     4681 tactaccatt tcaccccgtt caatatgctg atgaactggg gcagcgcttt tcgctggttt
     4741 gattcatcga ttggaccgag tctgcgcgtc tggtattacc gcgactattc tgatccggtg
     4801 cgcgtttcca cgcgtaacga tgactggacg cgcgcgactt tcatgattgt ttaccggcaa
     4861 tacatccagt tccatccgct ggtgcgcctg cgtacacact tctattttcc cgcattgagt
     4921 ttcttcggtg aaatggccaa gtcaccttac ttcaacgaaa aagaatatat actcaatacg
     4981 gcgctcgagt tctac
//
LOCUS       DAOQJF010000255         4993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_9906_length_4993_cov_4.839814, whole genome shotgun sequence.
ACCESSION   DAOQJF010000255 DAOQJF010000000
VERSION     DAOQJF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9906_length_4993_cov_4.839814"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1172
                     /locus_tag="PLF85_12535"
     CDS             126..1172
                     /locus_tag="PLF85_12535"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE20544.1"
                     /translation="MLFLPTLLFAAGFRTVARVERIDPVTHVAEVSAGVVLEPGKEYT
                     VLNESGEVIGAAKEVKRIGNGNSVFTFAGKRRSLSAGRRITLVAANYGFSALGDRPRQ
                     QSQPQMRFELKDRAAMVFVPEGPFILGGQQVNALHYVPAQLDGKATNRADVAAFFIDQ
                     HEVTVGQYRHYLEETRQRIPEDLVEKTSELPVTHLTYRQAENYCAWTGKRLPTELEWE
                     KAARGSQVATVADETFTEIRNYPVGDDAATERCVTAQNHSEPVEVSKLKDQSPYGLVG
                     MCGNAAEWTSSWLLPYRGNSARDERFGRRYKVIRGGSYEHSLELAKSYIRLAGGIPTL
                     ARDRRAGFRCARSE"
     gene            complement(1231..2589)
                     /locus_tag="PLF85_12540"
     CDS             complement(1231..2589)
                     /locus_tag="PLF85_12540"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNE20545.1"
                     /translation="MMFRRAASPAAVERLTLENGLTVLLENIPHRTSATVGLWLPVGS
                     RFEKKSEAGYSHFVEHMLFKGTKKRHYTEISRAIDRLGGHMNASTSKEITDYYVSLSG
                     RHLPIALDVLADMFFDSQFSKEEFNSEKKVILEELKMTEDQPDDYLFDLFYESALGDN
                     SLGRPVAGYATNISRSTRDGLYEYYRSHYGPRGTVLSLAGALWSSAKEKNDLLSNIRQ
                     NFDRHDHVLQGEPGYSSPHFSSPEFQAERQVKQRHFLHKSKDLEQVNFVLSLPGQRNE
                     VDQPADLQVLTHYLGGTMSSRLFVELREKKGLCYSVSTFHSQFLREGVWGIFCATSPE
                     KYSTAVETAIGELEALASGIDAGEIEESKSGLKGGIELAMESAYRRASYNARTWLYHG
                     ELRDWREHLARVDRVTPASFLPALRHLWRGASPAITGLGPAIKAGTKSKLQKLISAPL
                     FS"
     gene            complement(2596..4707)
                     /gene="pnp"
                     /locus_tag="PLF85_12545"
     CDS             complement(2596..4707)
                     /gene="pnp"
                     /locus_tag="PLF85_12545"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNE20546.1"
                     /translation="MQHDFKIHKESVQVNGLDYELETGKWARQADGSVLLRWGNMLLM
                     ANATGAKKSGEGLDFFPMTVEYREKYYAGGRFPGGFLKRENRPSDKEILVSRVIDRPI
                     RPLFPENYFNEVQIFITLLSAQKNFSADVHAITAASAALTISGLPFDGPVAGVRIARV
                     AGKFILFPNRDEIAASDINLALAGTIKAVTMIEGLADEASEEDMIAAVKHGHEEIKRL
                     CEMQLKLKAKAGKPRIDVPAAPDHSELRKRIADLGMAAMKQANEIKGKFERQAAIDAA
                     KKSVLEQFKPEFAALEKDAAEKQEKLVKSLIADLEVDVVRNQIFHDAVRADGRKLDEI
                     RPISVEVGVLPGAHGSALFTRGETQSLGVLTLGTESNAQGTDDVEGESSSRFYLHYNF
                     PPFSVGEVKRFMGPGRREIGHGKLAENSLAAVVPDAGRFPYVMRLVSEILESNGSSSM
                     ATVCSGALAMMDGGVPIKAPVAGIAMGLITEGDKFAVLSDIAGLEDHFGDMDFKIAGT
                     TKGITGFQLDTKVQGISYEIMEKAIHQAKAGREHILKVMAEALEKPRPQLKDNAPRIS
                     TMTIERDRIGELIGPGGKNIRSIIEKTGVEINVDDNGLVQIASNDAESARKARAMIEG
                     QFAEAEIGKIYEGTVRKITDFGAFIEIMPGRDGLCHISKVSAQRIANVSEVLKEGQKV
                     KVKVLAIDRQGKISLSIRDAE"
     gene            complement(4835..>4993)
                     /gene="rpsO"
                     /locus_tag="PLF85_12550"
     CDS             complement(4835..>4993)
                     /gene="rpsO"
                     /locus_tag="PLF85_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNE20547.1"
                     /translation="RLTGHFQSHSKDHHSRVGLLKMVGKRRRLLDYLRRNSVDRYKAL
                     IAKLNLRK"
BASE COUNT         1006 a         1371 c         1426 g         1190 t
ORIGIN      
        1 aattattcat ccactttttg gtgcgatgcg gtatagtctt ttgcttcggc cgcaggtctg
       61 ctgtgcattg ggaaagtgaa taaaagtgcc gaaaatagag tgtggtcccc tgccggttgc
      121 tcgccttgct atttctgcca accctgctgt tcgctgcggg ttttcgtaca gttgcccgtg
      181 tcgagcggat agatcccgta acccacgtcg ccgaggtcag cgcaggggtt gtgctcgagc
      241 ctggaaaaga atatactgta ttgaatgaat cgggtgaggt cattggcgct gcgaaagaag
      301 tgaagcgcat tggcaatggc aactcggtgt tcacatttgc cggcaaacgc cgctcgctca
      361 gcgccggtcg cagaatcacc cttgttgccg caaattacgg gtttagtgca ttgggcgaca
      421 ggccacggca gcagtcacaa ccacagatgc gatttgagct caaagaccgt gctgctatgg
      481 tcttcgtgcc agaagggccc tttattctcg gtggccagca ggtaaatgcc ctgcactacg
      541 ttccggctca gcttgacggc aaagccacta accgggccga cgtagcagca ttcttcattg
      601 accagcacga agtcacggta ggccagtacc ggcattatct tgaagagacc cgccaaagaa
      661 ttccagagga cctcgtcgaa aaaacgtctg aactgccggt gacccacctg acttaccggc
      721 aggctgagaa ctattgtgcc tggaccggca agcggctgcc tacggagctc gaatgggaga
      781 aagcagcacg cggtagccag gtggcgactg ttgccgatga aacctttacc gaaatccgta
      841 attatcctgt cggtgatgac gcggctactg aacgctgcgt tactgctcag aatcattctg
      901 aacctgttga ggtaagcaag ctaaaggatc agagcccata cggattggtc ggaatgtgcg
      961 gcaatgccgc cgagtggacc agcagctggc ttttgcccta ccgtggtaat tcggcgcgcg
     1021 atgaacgctt cggacggcgt tacaaggtta tccgcggcgg ttcatacgag cattccctcg
     1081 agctggcgaa aagttatatt cgcctggcgg gcggtattcc cactctcgcg cgcgacaggc
     1141 gtgcagggtt tcgttgtgca cgcagtgaat gaagaatgct ctggaaatcg cccgcgatat
     1201 ggccaccggt ggccggtttc caggcatatc ttagctgaac aacggagcgc tgatcagctt
     1261 ttgcagtttt gatttcgtcc ccgccttaat tgcagggcca agacccgtga tggccgggga
     1321 agctccccgc caaagatgac gcaatgcagg caggaagctg gctggtgtga cacggtcgac
     1381 ccttgccagg tgttcgcgcc agtcgcgcaa ttcaccgtga tacaaccagg tacgcgcatt
     1441 gtagctagcg cggcggtacg cactttccat ggcgagttca ataccacctt tgaggccaga
     1501 tttgctctct tcaatttcgc cggcgtcgat gccgctggcc agggcttcga gttcaccgat
     1561 cgcggtttct accgcggtcg aatacttttc aggcgaggtc gcacagaaaa tgccccagac
     1621 accttcgcgc agaaattgag aatgaaaggt gctgaccgaa taacaaagtc cctttttctc
     1681 ccgcagttcg acaaaaagac gcgatgacat cgtgccaccg aggtagtgcg tgagtacctg
     1741 caggtctgca ggctggtcga cttcattgcg ttggccgggc aaagacagca caaagttcac
     1801 ctgttcaagg tctttggact tgtgcagaaa gtgccgctgt ttgacctggc gctcggcctg
     1861 aaactcaggg ctgctgaaat ggggggagct ataaccgggc tcaccctgca gcacgtggtc
     1921 gtgccggtca aaattctgcc ggatattcga cagcaggtca tttttttcct tcgccgagct
     1981 ccacagtgcg cctgcaagcg agagcactgt accgcgcggt ccgtagtgag accggtagta
     2041 ttcgtagaga ccgtcgcgtg tgctgcgtga gatgttcgtt gcgtaacccg cgaccggccg
     2101 ccccagggag ttatcaccca gcgcgctttc ataaaacagg tcgaagaggt aatcgtcagg
     2161 ctggtcttcg gtcattttca gctcttcgag aatgaccttc ttctccgagt tgaattcttc
     2221 tttgctgaac tgcgaatcga agaacatgtc ggccaggaca tcgagcgcga taggaaggtg
     2281 gcgcccgctc aggctgacat agtagtcggt gatttctttg ctggtcgaag cattcatatg
     2341 cccgcccagt cggtcaatcg cccgggaaat ttcggtataa tgccgcttct tggtaccttt
     2401 gaagagcata tgctccacaa agtgcgagta acctgcttcg gatttctttt cgaaacgcga
     2461 gcctaccggc agccaaaggc cgacggtagc gctcgtgcgg tggggaatgt tttccagcag
     2521 aaccgtcagc ccgttctcga gcgttaggcg ctcgacagct gccgggctgg ctgctctgcg
     2581 gaacatcatg gtgccttatt ccgcgtcgcg gatcgacagc gaaattttgc cctggcggtc
     2641 gatcgccagt accttcacct tcactttctg gccttctttc aggacttcag aaacattggc
     2701 gatgcgctgc gcggagacct tgctgatatg gcaaagacca tcgcggcctg gcataatttc
     2761 gatgaaagcg ccgaagtcgg tgatcttgcg cacagtgcct tcatagatct tgccgatctc
     2821 tgcttcggca aactggcctt cgatcatcgc gcgggctttg cgtgccgatt ctgcgtcatt
     2881 cgaagcgatc tgcaccaaac cgttatcgtc gacattgatc tcgacgccgg ttttttcgat
     2941 gatcgagcgg atgttcttac cacctggtcc aataagctcg ccgatgcggt cacgttcgat
     3001 cgtcatggta gagatgcgcg gggcattatc tttgagctgc ggacgcggct tctcgagcgc
     3061 ttcggccatc accttgagaa tgtgctcacg gccggctttc gcctggtgaa tcgctttctc
     3121 catgatttcg tagctaatgc cctgtacctt ggtgtcgagc tggaagccgg tgatgccctt
     3181 agtggtgccg gcgattttga agtccatatc gccgaagtgg tcttcgagac ctgcgatatc
     3241 agacaagacg gcaaatttgt cgccttcagt gatcagtccc atcgctatac cggcaacagg
     3301 tgccttaatc ggtacaccgc catccatcat ggcgagcgcg cccgagcaga ctgttgccat
     3361 cgacgatgaa ccgttcgatt cgagaatttc agaaacgagg cgcatcacgt acggaaagcg
     3421 tcctgcatcg ggcaccactg ctgccaggga gttttctgcc agcttgccgt gaccgatctc
     3481 gcggcggcct gggcccatga agcgtttcac ttcgcccacg ctgaatggtg gaaagttgta
     3541 atgcaggtag aagcggcttg aagattcacc ttcgacgtcg tctgttccct gcgcgttgct
     3601 ttcagtgccg agagtgagaa ctcccagcga ctgcgtttca ccgcgcgtga agagcgccga
     3661 gccgtgtgcg ccgggcaata cacccacttc gaccgagatc gggcggattt cgtcgagttt
     3721 acggccgtct gcgcgcacag cgtcgtggaa gatttggttg cgcaccacgt caacctcgag
     3781 gtcggcgatg agagatttca cgagtttttc ctgtttttct gccgcgtctt tttcgagcgc
     3841 agcaaattca ggcttaaact gctcgagcac gctcttcttg gcggcgtcga ttgccgcctg
     3901 gcgttcgaat ttgcccttga tttcgttggc ctgcttcatt gcagccatgc cgaggtcggc
     3961 aatgcgcttg cgcagttcgg agtggtctgg tgctgccgga acgtcgatgc gcggcttgcc
     4021 ggctttcgcc ttgagcttca gctgcatttc gcagaggcgc ttgatttctt cgtggccatg
     4081 tttcactgcc gcgatcatgt cttcttcgct cgcttcgtca gcaagaccct cgatcatggt
     4141 gactgcctta atcgtgccgg cgagcgcgag gttgatgtct gacgccgcga tttcgtcgcg
     4201 gttcgggaaa aggatgaact tgcctgcgac gcgtgcgata cgcacaccgg ccaccggtcc
     4261 gtcgaatggc aggcctgaaa ttgtcagcgc tgcagaagcc gcggtaatcg cgtgcacgtc
     4321 ggcgctgaag ttcttctgtg cgctcagcag cgtgatgaag atctgtactt cgttgaagta
     4381 gttttcagga aagagcgggc gaatcgggcg gtcgattacg cgcgagacca ggatttcttt
     4441 gtcagaagga cgattctcgc gcttcaggaa gccaccggga aaacggccac ccgcgtagta
     4501 cttctcgcgg tattcaactg tcatcgggaa gaaatcgaga ccttcgcccg atttttttgc
     4561 cccggtcgca ttcgccatga gcagcatgtt gccccagcgc agcaggacag aaccatctgc
     4621 ctggcgtgcc catttgcccg tctcgagttc gtaatcgagc ccgtttacct ggacactttc
     4681 tttgtggatt ttaaagtcgt gttgcatttg tttctccaat ctatagcccg cacaggaaga
     4741 accctgaata gaggtcttcc tgtacggaca tttcttaagc ctagaggtcc cttgcgtcgc
     4801 aagcgggccc ctcaggcccc gtcacaaggg ttagttactt acggagattg agtttagcga
     4861 tcagcgcctt gtagcggtca acgctgttac ggcgcagata atcgagcaag cggcgacgct
     4921 tacctaccat cttcagcaga ccaacgcgtg agtggtggtc tttgctatgg ctctgaaagt
     4981 ggcccgtgag gcg
//
LOCUS       DAOQJF010000256         4964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10001_length_4964_cov_4.355266, whole genome shotgun sequence.
ACCESSION   DAOQJF010000256 DAOQJF010000000
VERSION     DAOQJF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10001_length_4964_cov_4.355266"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PLF85_12555"
     CDS             complement(<1..997)
                     /locus_tag="PLF85_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE20548.1"
                     /translation="MDLKEYGILSAHQLGFQWVTADPFLFCVHHLDQYPRGNEILGPA
                     ATLAGRNIGQDFEPRDGWRMYHGERVPGFPGHPHRGFETITVVRRGVVDHSDSMGAAG
                     RYGAGDVQWMTAGAGVQHAEMFPLLEREKDNTLELFQIWLNLPAKSKFAKPHFKMLWQ
                     NQIPVVTEKDASGRETRIELVAGSYAGVNPPAPPPDSWAADAANAVNVWLIDMPAGAQ
                     WKLPAAAAGLNRFAYFYEGDNLVISGGVIPAGTGLRLMSERQFVLEAGAQGAKILLLQ
                     GRPIAEPVVQYGPFVMNSRQEIEQAFSDYQRTRFGGWPWPSDEPVHGRDRGRFARH"
     gene            1252..2130
                     /locus_tag="PLF85_12560"
     CDS             1252..2130
                     /locus_tag="PLF85_12560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNE20549.1"
                     /translation="MADRYGALKYIQTLDPEKDAHQIVAVSSAFDFAQDMEISLGLAF
                     FRTFAIPSIARILDETKQFEKFGQKRYDDTTILLAEFLENGLDSERGREAIRRMNQIH
                     RQYNIPNDDYLYTLTTFIFEPIRWIDRFGWRPLDEKERLAAFYFWRRVGEMMGIREIP
                     GNYAELEAFNVRYEREKFRYTPESDRVSQATLAVLEGFLPRLPGLGFLAREGIYALCD
                     APLRRAVGFPDPNPLVLAAVEAAFKARAFFLRHLGQPPRDPERITARKFSTYPQGYRI
                     AEVGPAAVPTAAQQTA"
     gene            2226..4085
                     /locus_tag="PLF85_12565"
     CDS             2226..4085
                     /locus_tag="PLF85_12565"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNE20550.1"
                     /translation="MQKPDFVTNQRLITWVEAMANLTQPDSIHFCDGSDEEYDRLCAE
                     MVAKGTFIRLNPEKRPNSYLAWSDPSDVARVEDRTFICSKRPADAGPTNNWKDPREMK
                     GILNNLFNGCMRGRTMYVVPFCMGPIGSKMSLYGVQITDSAYAVVSMKIMTRMGKEAL
                     DAIGDGNFIPCMHSVGAPLAPGQKDVAWPCNKDNKYIVHFPEERAIWSYGSGYGGNAL
                     LGKKCLALRIASVIGRDEGWLAEHMLILGMEKPDGAKTYVAAAFPSACGKTNFAMMVP
                     PEHFKGYKVTTVGDDIAWVYPAEDGGLVAINPEAGYFGVLPGTSKESNRNAMEIISKN
                     TIFTNVALTKDGDVWWEGMTKEIPEGITDWTGQPWTPDCGRKAAHPNARFTSPASQNP
                     AIDKDFDNPHGVKISAFVFGGRRSTTVPLVFQAVNWNHGVYFAATIGSEMTAAAAGTI
                     GQVRRDPFAMLPFCGYHIADYFNHWLQMGRNLGNPPRMFFVNWFRKDNDGKFIWPGFG
                     ENTRVLEWIVDRVHGKGTATESPLGWMPRYEEMDWEGLSFTREQFNQLMTIDTRLWKN
                     EVLQHEEFFDSIYDKLPKELFWIRQLLLSSLWRSGEKWLVGGNLGITTRASDS"
     gene            4115..>4964
                     /locus_tag="PLF85_12570"
     CDS             4115..>4964
                     /locus_tag="PLF85_12570"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNE20551.1"
                     /translation="MRFLQAGSRQRSLFFFLLLVVWGCRRGEVAHSAAAHWLALPAEL
                     QGEVQGMPPHWSVQGTGGAVSSANPLATAAGIEVLQSGGNAIDALLTVQWVLSVTEPQ
                     SSGLGGGGFLVYYDARSKTAYALDGREEQPAAAPASLFLDGSGKPLPFAERIAGARAI
                     GVPGTVALLDHALRRFGSGKYRWQSLFDRAIALAQNGIRVSPRLAQAMLLNRDRLLKQ
                     NGDSSAYLRSRQPYQVGEAFYQHDLAATLRRLAEKGAADFYTGAIAADLLATVQGAVE
                     ARAAHHS"
BASE COUNT         1029 a         1473 c         1441 g         1021 t
ORIGIN      
        1 cgtggcgcgc gaaacggccg cggtcacggc catgcacggg ctcgtcgctt ggccacggcc
       61 agccgccaaa gcgcgtgcgc tggtagtccg aaaaagcctg ctcgatttcc tgccggctgt
      121 tcatgacgaa ggggccatac tgcacgacgg gctcggcaat cggccgtccc tgcagcagca
      181 gaatctttgc accctgcgct cctgcctcga gcacgaattg cctctcgctc atgagccgca
      241 ggcctgtgcc cgcagggata actccccccg aaatcaccag gttatcaccc tcgtagaaat
      301 aggcaaagcg gttgaggccg gctgccgcgg ccgggagctt ccattgtgcc ccggccggca
      361 tgtcgatgag ccatacattc accgcattcg ccgcatcggc cgcccatgaa tcgggcggtg
      421 gtgcaggggg attaaccccg gcgtaactgc cggcaacgag ttcgattctt gtctcgcggc
      481 ctgacgcatc tttctcggtc acgaccggaa tctggttctg ccagagcatt ttgaaatgcg
      541 gctttgcaaa cttgctctta gccggcaggt tgagccagat ctggaacagt tcgagcgtgt
      601 tgtctttttc ccgttcgagc agcgggaaca tttcggcgtg ctgcactccc gcgccggcag
      661 tcatccactg cacgtcgcct gcgccgtaac ggcctgcagc tcccatcgaa tcggaatgat
      721 cgacaacccc gcggcgtact accgtaatgg tttcgaagcc gcggtgcggg tgcccgggaa
      781 aacccgggac gcgttcaccg tggtacatgc gccagccgtc gcgcggttca aaatcctggc
      841 cgatgttgcg acccgcaagg gtggcggctg gtcccagtat ctcattgcca cgcgggtatt
      901 ggtcgaggtg gtgcacgcag aacaggaaag ggtctgccgt tacccattga aagcccagct
      961 ggtgggccga taaaataccg tattctttca gatccatggt cggtaaattc tccagcgaag
     1021 gcgtgcggtt gcggcgaaag aacaattgcc agccaccggc gaacagtgtc gcggtaccag
     1081 caagggccgt aagaaatttc tttcgtgtca tgcgcgcctt cagtgtcaag ataatgctcc
     1141 ggccacaatt gctccacaaa tctgttggcg ggctcctgaa aaggagcccg aagcaggttc
     1201 ttggtaatgc ggctttatgg gcgaggaaac tattttgcgt atattagtac aatggcagat
     1261 cgatatggtg cgttgaaata tatccagacg ctcgacccgg aaaaagacgc gcaccagatt
     1321 gtcgcagtct caagcgcgtt tgatttcgcg caggatatgg agatttcgct cggcctggcc
     1381 ttcttcagga ctttcgcgat cccctcgatt gcgcgaatcc tcgatgaaac caaacaattt
     1441 gaaaaatttg gccagaagcg ctacgacgat accacgatcc tgctcgcaga atttctcgag
     1501 aacggcctcg acagcgagcg cggtcgcgag gccattcggc gcatgaacca gatacaccgg
     1561 cagtataaca ttcctaacga tgactatctc tataccttga cgaccttcat ctttgaaccc
     1621 attcgctgga tcgaccgctt tggctggcgg ccactcgacg aaaaggaacg gcttgcggcg
     1681 ttctacttct ggcgccgtgt cggcgagatg atgggcatca gggagattcc cggcaactac
     1741 gccgaactcg aggccttcaa cgtgcgctac gaacgcgaga aatttcgtta cacgcccgaa
     1801 tctgaccggg taagccaggc gacgctcgcc gtgctcgaag gttttctgcc gcgtttacct
     1861 ggcttgggat ttcttgcccg cgaaggtatc tacgccctct gcgacgcacc gctcagaagg
     1921 gcagtggggt ttcccgaccc caacccgctc gttttggcgg cggtagaggc ggccttcaag
     1981 gcgcgcgcat ttttcctgcg ccacctgggg caacctccca gggaccccga gcgcattacg
     2041 gcgcgcaagt tttctacgta cccgcagggt taccgcatcg ccgaggttgg cccggcggca
     2101 gtaccaaccg cagctcagca aacagcgtga ttgggtttga gcaggtcctg ccgaagccca
     2161 attcgctgcg cggcgggtct tgcacttgac ggcttgaatt tgaaacaggt ttcctctaag
     2221 ctcccatgca aaaaccggat ttcgtgacga accagcgcct cattacctgg gttgaggcga
     2281 tggctaacct gacccagccc gattccatcc atttctgcga cggctcagac gaagaatacg
     2341 accgcctgtg cgccgaaatg gtcgccaaag gtaccttcat caggctgaat cctgaaaagc
     2401 gccccaacag ctatcttgcc tggtctgacc cgtcagacgt ggcaagggtc gaagaccgca
     2461 cttttatctg cagcaaacgc cccgcagacg caggcccgac caacaactgg aaagacccgc
     2521 gcgagatgaa gggcatactc aataacctgt ttaatggctg tatgcgcggc cgtaccatgt
     2581 acgtcgtgcc attctgcatg gggcccatcg gctcgaaaat gtcgctctat ggcgtacaga
     2641 ttaccgactc ggcttacgcc gttgtcagca tgaagatcat gacccgcatg ggcaaagaag
     2701 cgctcgatgc aatcggcgac ggcaatttta ttccctgcat gcactcggtg ggagctcccc
     2761 ttgctccggg ccagaaagac gtggcatggc catgcaacaa agacaacaaa tacatcgtgc
     2821 actttcccga agagcgcgcc atctggtcat acggttcggg ttacggcggc aacgcactgc
     2881 tcggtaaaaa atgcctcgcg ctgcgtattg cttcagtgat tggccgcgac gaaggctggc
     2941 tcgcagagca catgctcatt cttggtatgg aaaagcccga cggcgcaaag acctatgtcg
     3001 ctgctgcatt cccgtcagcg tgcggtaaaa ccaacttcgc gatgatggtg ccgcccgagc
     3061 actttaaagg atacaaggta accaccgtgg gcgacgacat tgcctgggtt tacccggctg
     3121 aagacggcgg tctcgtggcg atcaatcccg aagcaggtta ttttggcgtg ttgccgggta
     3181 cctcaaaaga atcgaaccgc aacgcgatgg aaatcatcag caagaatacc atcttcacca
     3241 acgtggcgct caccaaagat ggcgacgtct ggtgggaggg catgaccaaa gaaattcccg
     3301 aaggtattac cgactggaca ggccagccgt ggactcccga ttgcggccgc aaggcagcgc
     3361 acccgaatgc gcgctttacc tcgccggcaa gccaaaaccc cgcgatcgac aaggactttg
     3421 ataacccgca tggtgttaaa atcagcgcgt tcgttttcgg cgggcgccgc tctacaaccg
     3481 taccactcgt cttccaggca gtaaactgga accacggcgt gtacttcgca gcaacaatcg
     3541 gttcagagat gaccgcggct gctgcaggaa ccattggcca ggtacgccgc gacccgttcg
     3601 cgatgctgcc gttctgcggt taccacatcg cagactattt caaccactgg ctgcagatgg
     3661 gacgtaacct gggtaatcca ccccgcatgt tcttcgtcaa ctggttccgc aaggataacg
     3721 acggcaagtt catctggccg ggttttggcg agaacacccg tgtactcgaa tggattgtcg
     3781 atcgtgtaca cggcaagggc actgcgaccg agagcccgct cggctggatg ccgcgctacg
     3841 aagagatgga ctgggaaggt ctttcgttca cgcgcgagca gtttaaccag ctgatgacca
     3901 tcgacacgcg cctttggaag aacgaagtgc tgcagcacga agaattcttc gacagtattt
     3961 acgacaagct gcccaaagaa ctcttctgga ttcgccagct gttactctcg agcctctggc
     4021 gctcgggcga gaagtggctc gtgggcggca acctcggaat taccacccgc gccagcgact
     4081 cataaaagtg cgcgtgccgg cacgcgcagg tttgttgcgg tttctccagg caggctcccg
     4141 tcagcggagc ctgttctttt ttctactgct cgtcgtttgg ggctgccgtc gcggtgaagt
     4201 tgcgcatagt gcagcggcgc actggctggc tttgcctgca gagctgcagg gtgaagtgca
     4261 gggcatgcca ccgcactggt ctgtacaggg tacgggtggt gcagtctctt cggcgaatcc
     4321 gctggcgacc gcggcaggta ttgaagtgct gcaatcgggc ggcaatgcga tcgatgcatt
     4381 gctgactgtg cagtgggtac tctccgtgac agaaccgcag tcgtcgggcc ttggcggcgg
     4441 gggatttctg gtctattacg atgcccgttc gaaaactgcc tatgctctcg atggccgcga
     4501 agagcagccc gctgctgcgc cggccagcct gtttctcgat ggctccggta agccattgcc
     4561 ctttgccgaa cgtattgccg gcgctcgcgc tatcggtgtg ccgggtacag tcgcattgct
     4621 ggaccatgca ctgcgccgct ttggttctgg taagtaccgc tggcaaagcc tgttcgatcg
     4681 tgctatcgcg ctcgcgcaga atggcatacg cgtctctccg cgcctcgcgc aggcgatgct
     4741 gctcaaccgt gaccgcttac tgaaacagaa tggcgacagc tccgcctacc tgcgtagcag
     4801 gcagccctac caggttggcg aagcatttta ccaacatgac ctggcggcaa cgctgcgcag
     4861 gcttgcagag aaaggcgccg cggattttta tacgggggcg attgctgccg acctgctggc
     4921 caccgtgcag ggcgcggtgg aggcgcgcgc cgcgcaccac agcc
//
LOCUS       DAOQJF010000257         4892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10204_length_4892_cov_5.103990, whole genome shotgun sequence.
ACCESSION   DAOQJF010000257 DAOQJF010000000
VERSION     DAOQJF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10204_length_4892_cov_5.103990"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1333)
                     /gene="carA"
                     /locus_tag="PLF85_12575"
     CDS             complement(182..1333)
                     /gene="carA"
                     /locus_tag="PLF85_12575"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNE20552.1"
                     /translation="MSAVRPGVLVFSDGTFFRGTLFGADLAKAGSFGEAVFQTAMSGY
                     QEILTDPSYRGQIVCFTYPSFGNYGVNPVDFESPRAWLSGVVVRDLCEIPSNFRSTAT
                     VDQFLREQNVAGITGVDTRAVVRKIREGGAQTAGIFSAEGADMAELARKVAAQPSMDG
                     LNLVKDFDGKAANEFVAAYLKEKNIHPTSLKPIAVLDFGIKYNILRELVAQGFMPRVF
                     AGDTPLENQKDFQASDYVGYFFSNGPGDPAVVESGIRNIRQLIDTGKPCLGICLGHQM
                     ISHALGAKTYKLKFGHHGGNQPVKADYRKQVIITSQNHGFAVDEESLRKVTSAGAKFE
                     NNANDLSAEGFRLVDEKRKVLSVQYHPEAAPGPRDAAIVFSEFRALFTN"
     gene            complement(1401..2750)
                     /locus_tag="PLF85_12580"
     CDS             complement(1401..2750)
                     /locus_tag="PLF85_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HNE20553.1"
                     /translation="MRSILQKTVENFGLADAEKNFRNTFIEKNRGKPLFLMLVENLKD
                     MRIDEFLGRIPDFIKRVNSEEKQSIHCYVLGDESRFYVGIVPGDDAPGFLPNIDGAMG
                     KFHGEAPKDGDGSFDFGIGRTQCNYISDAQEIFDELVRAAHKNLKDNLVRWSWTYLNR
                     ANDYFANVEANAVIQPIIYFDPRKETYSVKGGEVFVGGELYNSYAELLRDIPDDQDIN
                     RVELLILEKIVMACHEAPGLLKFNVSPQTLIDTFPSAEKVFRFNELIARQKINPHNVR
                     LELVEKPYEEKTIKLKDVCRYFFQFGITFAADDFGVKSQSHQVILDLGEMIKEFKLDP
                     ISFKFKPEEDQTKFLDNLAFIDYCIRLADNREAIITAEAVDDYDTMRFLIAHQVFYFQ
                     SHIFCRKLPIHYYKENFHKLQNLPESAVRKILSEDELIQKQKEVGNIFELARQLGLA"
     gene            2881..3417
                     /locus_tag="PLF85_12585"
     CDS             2881..3417
                     /locus_tag="PLF85_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20554.1"
                     /translation="MSWLRTLVLAGLANLLLHPLYPQSGAAQVVDMTDAVGPLEQEQA
                     DLSINLAHSLAREKRYGEALQAYSEFLRVFPVNLRSREARESMARIYEKRQRYDLAIR
                     QYDALYRELGISQQGLAYRLEAARLQEMSGDETAAAAIYREINQLDPGSEAGIKARTR
                     MEALNLMQKTANPAELAN"
     gene            complement(3410..4300)
                     /locus_tag="PLF85_12590"
     CDS             complement(3410..4300)
                     /locus_tag="PLF85_12590"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE20555.1"
                     /translation="MLTDKLKLLELPPMPQVAARVMQIDESQISVSAEQIRNIVQSDL
                     ALSTKILKLANSAFYARGNRINNLSQAIALLGFKTVKGLSLLVSAASMIPKKGNFKIM
                     KELWMRSVLTALISKIIAEKVGKEKQKDEVFMIGLLRQIGKSILANQFAEEYTICFQM
                     SADAADDEKLHQLEQMKWQFNSAEASEFAMKTWNFPPELIAASKVLRYRIEDPVALEK
                     EQPLLMPVILAEYIVLLSGYTDTFKPDAKLREKLLAEFGRVCPVYNIDETRKKYYLED
                     LRQVIKQDSFYSFCGELFSN"
     gene            complement(4418..>4892)
                     /locus_tag="PLF85_12595"
     CDS             complement(4418..>4892)
                     /locus_tag="PLF85_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNE20556.1"
                     /translation="ATLLGRLRAGIELYQKQIVPKLLLSGDNTRKFYDEVHTMRDYCL
                     RHGVKPEDIFLDHAGVRTYDTMARARRTFGVRTAIIVSQQLYLPRALFLAGAVGIKAE
                     GYVAESSSFDNTWQALAREYLARFKSLLDIYLLKPAVPQESPQTITGDGRETWKK"
BASE COUNT         1075 a         1308 c         1360 g         1149 t
ORIGIN      
        1 cttgttcagc tggtccgggt tacgcgaagt acggatgatt ccttcgtatt gccgtgatag
       61 tttttgcagc gtcaggagct cattctgctg cgcttcggtg agagacatag tttactcgga
      121 tgtgtgcggc ggcgtatgtg taaagcaaat tcagcccgcc aattggccgg ccaccggccg
      181 gtcaattggt gaaaagtgcg cggaattcag aaaagacgat ggctgcgtcg cgtgggccag
      241 gcgctgcctc aggatggtac tgtacgctca ggactttgcg cttctcatcg acgaggcgga
      301 atccttcggc gctcagatcg ttggcgttgt tttcgaactt cgcacccgcc gaggtcacct
      361 tgcgcagcga ctcttcgtct acggcgaagc catggttctg cgaggtaatg atcacctgct
      421 tgcggtagtc tgctttgacc ggctggttac cgccgtggtg gccaaacttg agcttgtacg
      481 tttttgcacc cagggcatgc gaaatcattt ggtgcccaag gcagataccg aggcagggtt
      541 tgccggtgtc tatcagctgg cggatattac ggataccact ttccaccacg gcagggtcac
      601 ctggaccgtt gctgaagaag tagccgacgt agtcgctggc ctgaaagtct ttctggtttt
      661 cgagcggggt atcaccggca aaaacacggg gcatgaagcc ctgcgcaacg agttcgcgca
      721 ggatgttgta tttaatgcca aaatcgagta cggcaatcgg cttgagcgaa gtgggatgga
      781 tatttttttc tttgagatag gccgcgacga attcattggc cgccttgccg tcgaaatctt
      841 taacaaggtt aagaccatcc atcgaaggct gagcggccac cttgcgtgcc agttcggcca
      901 tatcggcgcc ctcggcagaa aatatgcccg cggtctgcgc tcccccttcc ctgattttgc
      961 gcacgactgc gcgcgtatca acacccgtta tgccggctac gttctgctca cgcagaaact
     1021 ggtctacagt cgccgtcgac cggaagttac tgggtatctc gcacaggtcg cgcaccacga
     1081 caccggaaag ccaggcgcgc ggcgactcga agtctaccgg gttaacgcca taattgccaa
     1141 aagagggata agtaaaacag acgatctggc cgcggtaact ggggtcggtc aggatctcct
     1201 ggtagccaga catcgccgtc tgaaagaccg cttcgccgaa tgaacccgct ttggcgaggt
     1261 ctgccccgaa cagggtgcca cggaaaaaag tgccgtcgga gaagaccaaa acccccggac
     1321 gcactgcact cacaccgaca aactgcagaa gcgactcgcg cagcaaagca caaagagaaa
     1381 acgggtaagc accggcggcg ctaggctaat cccagttgcc gcgccagttc aaagatattg
     1441 cccacctctt tctgcttctg tataagctcg tcttcagaga ggatcttgcg aaccgcactt
     1501 tcgggcaaat tctgcaactt gtggaaattt tctttatagt agtgaatggg cagcttgcgg
     1561 cagaagatat ggctctgaaa atagaatacc tggtgcgcta ttaagaaccg catcgtgtca
     1621 tagtcatcga ccgcttcagc ggtgatgatc gcttcgcggt tatccgccag ccgaatacaa
     1681 taatcgatga aagcaaggtt gtccaggaac ttggtctggt cttcttcggg cttgaatttg
     1741 aagctgatgg gatccagttt aaattctttg atcatttcac cgaggtcgag aatcacctgg
     1801 tggctctggc tcttgacgcc gaagtcgtct gcggcgaagg tgataccaaa ttggaagaag
     1861 tagcggcaga catccttgag cttaattgtt ttctcttcgt agggtttctc gaccagctca
     1921 aggcgcacgt tgtgcggatt gattttttgc cgggcaatga gctcgttgaa acggaatacc
     1981 ttctctgctg acgggaaagt atcgatcagc gtctgcggcg aaacgttgaa tttcaaaagg
     2041 cctggcgctt cgtggcaggc catgacgatt ttttcgagaa tcagtagttc gacgcggttg
     2101 atgtcctggt cgtcaggaat gtcgcgcagg agttcagcgt agctattgta gagctcgcca
     2161 ccgacgaaaa cctcgccgcc ctttactgaa taggtctctt tacgggggtc gaagtaaata
     2221 atcggctgta tgacggcatt ggcttcaacg ttggcgaagt agtcgtttgc gcggttgagg
     2281 tacgtccagc tccagcgcac caggttgtcc ttgaggttct tgtgcgcggc acgcacgagt
     2341 tcatcgaaaa tttcctgcgc gtccgaaata taattacatt gggtgcggcc gataccgaaa
     2401 tcaaaactgc cgtcgccatc ttttggcgcc tcgccgtgga acttacccat cgcgccgtcg
     2461 atattcggca ggaagcccgg tgcatcgtca cccggcacga tgccgacgta aaagcgtgat
     2521 tcatcgccga gcacataaca gtgaatcgac tgcttctctt cggaattcac acgctttatg
     2581 aaatcgggta tcctgcccag gaactcgtcg atgcgcatgt ccttgagatt ctcgaccagc
     2641 atcaggaaca gcggcttgcc gcggttcttt tcaataaatg tattgcgaaa gtttttttcg
     2701 gcgtcggcaa ggccaaaatt ttcgactgtc ttctgtaaaa tagatcgcaa gggaagcgcc
     2761 accgtgtata cagctatatg ctttgccata ggctgaaaag gccaaaacaa tgaaaaatcc
     2821 ccattacccg ccagccgcca gcgctaaagg tttttagcaa atttgccgat gtgacataat
     2881 atgtcgtggc tccgcaccct tgttctcgca ggtctcgcga acctgctttt gcacccgctg
     2941 tacccgcaat caggcgccgc gcaggtcgtt gacatgaccg acgccgtggg tccgctcgag
     3001 caggagcagg ccgatttgtc gatcaacctg gcgcactcgc tggcacgcga aaagcgatat
     3061 ggtgaagcct tgcaggctta cagcgaattc ctgcgggtct ttcctgtcaa cctgcgcagc
     3121 cgtgaagcac gcgaaagtat ggcccgcatc tatgaaaagc gccagcgcta cgaccttgcg
     3181 atcaggcagt acgatgcgct ctaccgcgaa ctgggtatca gccagcaggg gcttgcgtac
     3241 cgtctcgagg cggcgcgcct gcaggaaatg tcgggcgatg aaacggccgc cgccgcgatc
     3301 taccgtgaga ttaaccagct tgatccgggc tcagaagccg gcataaaggc gcgcacgagg
     3361 atggaagcac tcaacctgat gcagaaaact gccaacccgg cagaactggc taattagaga
     3421 agagttcgcc gcagaagctg tagaaggagt cctgcttaat aacctggcgc aggtcttcaa
     3481 gatagtattt cttgcgcgtt tcatcgatat tatagaccgg gcagacccgg ccaaactcgg
     3541 ccagcaactt ttcacgtaac ttcgcatcag gcttgaaggt atcggtatac ccggaaagca
     3601 acacaatgta ctccgcgaga attaccggca tcagcaacgg ctgttctttt tcgagcgcca
     3661 cggggtcttc aatacggtag cgcaggacct tgctggccgc aatcagttca gggggaaaat
     3721 tccacgtctt catcgcgaat tccgatgcct ctgccgaatt aaactgccat ttcatctgtt
     3781 cgagctggtg gagtttttcg tcgtcggctg catcggccga catctggaag cagatcgtgt
     3841 attcttcggc aaactggttc gcgagaatgg atttgccgat ttgccgcagc agaccgatca
     3901 taaagacttc gtccttctgc ttttctttgc ctactttttc ggcgatgatc tttgaaatca
     3961 gggcagtgag caccgaacgc atccagagct ctttcataat cttgaaattg ccctttttcg
     4021 gtatcatcga agctgccgac accagcagcg atagcccctt aactgtttta aatcccaaca
     4081 gggctatggc ctgcgagagg ttattgatgc ggttgccccg cgcataaaaa gccgaattgg
     4141 cgagcttgag tatcttcgtc gatagcgcca ggtcggactg gacaatgttg cgtatctgct
     4201 cggccgatac gctgatctgg ctttcgtcta tctgcatgac gcgtgccgcg acctggggca
     4261 tcgggggcag ctcgagcaac ttgagcttgt cggtcagcat gtcgttcccc atggcatttt
     4321 cgattttgct gtgtcaatag attctcgagc aagttacgga acctgttttt ggcccctttt
     4381 caggagcctg ctgccaggct cctgaaaagg ggctttgcta ttttttccag gtctcgcgcc
     4441 cgtcaccggt aatggtttgc ggagattcct gcggcacggc cggcttcagc aaatagatat
     4501 cgagcagcga tttaaacctg gcaaggtact cacgcgccag cgcctgccag gtattgtcaa
     4561 agctggatga ctcggcgacg taaccttcgg ccttgatacc caccgccccg gccaggaaaa
     4621 gcgcccgtgg caggtagagc tgctggctga caatgatagc ggtgcgcaca ccgaaggtcc
     4681 gccgtgcacg cgccatggtg tcatacgtcc ggactcccgc gtggtcgaga aagatgtctt
     4741 ctggtttgac cccgtggcgc aggcagtagt cgcgcatggt gtgcacttcg tcgtagaact
     4801 tgcgggtgtt gtcgccggag agcagcagct tgggcacaat ctgcttctgg tagagctcga
     4861 tgccggccct gaggcgcccc aacagcgtgg cg
//
LOCUS       DAOQJF010000258         4890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10212_length_4890_cov_6.027508, whole genome shotgun sequence.
ACCESSION   DAOQJF010000258 DAOQJF010000000
VERSION     DAOQJF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10212_length_4890_cov_6.027508"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLF85_12600"
     CDS             complement(<1..595)
                     /locus_tag="PLF85_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20557.1"
                     /translation="MNLRLAILVAVLSGSSALFAAPVTAIGTFTRRLDSPVSKEVILR
                     FPIFLSKAYTRAQKGRIMYPNEFIRLREENKIPSKILYTPDEYAAITKVLNGGSLVTG
                     EIYEYEPKKPGEETDDETAVDIPKEKMVFVRIHHYNSETRKIVSFSVYSKPETIVIDL
                     VRHLLPLEKDRYIVRPIAAGSKIAVVAPLDDVSLNNFL"
     gene            complement(619..1209)
                     /locus_tag="PLF85_12605"
     CDS             complement(619..1209)
                     /locus_tag="PLF85_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20558.1"
                     /translation="MTKASVFLAVALATALTLPGCRSAANNYVIKEPIPKNSNVAVLV
                     EGENDFKNAVFMEFMRGGYDVKAFNATDFYTTDDVLDVRSLKKTAFVTNLFDPKNSQR
                     QVAVADKVFENIYKLHIFNFENLKLEMLQGLQKKLGIRYVILLSLSRWDAGYTWARVI
                     KLDTMQLVYVQNYNVSRKDDLKSVIGSMISTMQAGK"
     gene            complement(1297..2481)
                     /locus_tag="PLF85_12610"
     CDS             complement(1297..2481)
                     /locus_tag="PLF85_12610"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNE20559.1"
                     /translation="MLVIDGLGVGEQPDAYLYGPRGANTLSHIAELKAPLQIPNLSRM
                     GITNLTYLKSWPREEDTIGFYAKVTQRNEGNDSIGGHRELLGIYQEEKYHTFPRGLPP
                     EILDELSAITGRSFLGNANDVQQGFLTKYGETHLDTGAPILLVTDDSLINIYVHPDKF
                     QPDEFYLLGHSAWEVLSRHGLGRLHLHYFTGDLATFAPDETMPTGMFHSPPRAPTLAT
                     NLAHAGIPVHAIGKVSEMLEGAAITEQYAVKNNADCLEILTQAIREGTTNRNKQSFIV
                     ATLPDLDSLYAHNRDAMGYIDALEALDAYLPRIFRAMENEDMLYIVSDHGNDPTFEGT
                     SHTREYVPLLVYSRMFRPRRQANLGIRSTLADVAETIADSYDLNVRFAATSFWENMIS
                     QL"
     gene            2570..3406
                     /locus_tag="PLF85_12615"
     CDS             2570..3406
                     /locus_tag="PLF85_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE20560.1"
                     /translation="MAQTLPVCAMPASRFLPPTLLFAAFFTACQPQKRLEIAHAQGKK
                     QVSLSELRQRVSDREVEVDDHYLQRRTSYRALPLAPLLRAWAPAGPYDEVLFHCADGY
                     EARASRADLEQGRLDSFFLAYGEGGERFRSMIQQGKSQVSPEPFYAVSTDPKGYATLS
                     WPYEIVGVEFIRFADKYPGLLPDAVAQNAVTRAGFDLFRKECLRCHSLNLMGGEIGPE
                     LNVPRNITEYRDDAYLRAFIRNASAFRARSKMPPFTQLTDVELGKVIGYLKVMARFKV
                     PN"
     gene            complement(3425..4435)
                     /gene="mltG"
                     /locus_tag="PLF85_12620"
     CDS             complement(3425..4435)
                     /gene="mltG"
                     /locus_tag="PLF85_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNE20561.1"
                     /translation="MLQDNKQNFRKLLLFGGGGLGAVTLLLLAMLWLSLRSVSDGSRH
                     VDFAVKAGSNLSSVSSRLAEANLIHSQFFFKMYMRLTGKAARIKFGVYDLNDGMSAGK
                     IAQAITSGKTKTISITLAEGLHNRQIADLFVEKKFFASRDEFLQYASKKAILDKFKIP
                     AQTVEGYIFPDTYHLPQGYPKDKIIEHMVEHFYEKVKEVKNFPTEPQKIHQTVILASI
                     VEREAKLKEERPLIAAVFHNRMRDSEPLESCATVQYLFEKPKARLFYKDLEVESPYNT
                     YKVRGLPPGPIGSPGIAALDATVNPEKTDYKFFVLKGDGAHHFSKDFREHNAAKKKYI
                     GP"
     gene            4510..>4890
                     /locus_tag="PLF85_12625"
     CDS             4510..>4890
                     /locus_tag="PLF85_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20562.1"
                     /translation="MQKLLSPLVLILTLVFHCRSSGSADLARYAGKGANSENGYLTFY
                     AQGQKLSARTQATGKLRAYLLAAMREPDLKLSCSAWDCVAAALSSMSSSDRSPLYAYA
                     VYHLAAFAEQQNYPREASEILAQVG"
BASE COUNT          984 a         1332 c         1425 g         1149 t
ORIGIN      
        1 ctaagaagtt gttgaggctc acgtcatcga gcggcgccac cacggcgatc ttgctgccag
       61 ctgcgatggg gcgtacaata tagcggtctt tttcgagcgg cagcaggtga cgcacaaggt
      121 cgatcactat agtctcgggc tttgaataga cgctgaaaga gacgatcttc cgagtttctg
      181 aattgtagtg gtgaatacgc acgaagacca tcttttcttt gggaatatcg acggccgtct
      241 catcgtctgt ttcttcgccg ggttttttcg gctcgtattc gtaaatctcg cccgtgacga
      301 gcgaaccacc attcagtact tttgtaatcg ccgcgtactc gtcgggtgta tagagtatct
      361 tcgatggaat cttgttttct tcgcgcagcc tgataaattc gttcgggtac atgatgcggc
      421 ctttctgcgc ccgcgtgtac gccttgctca ggaatatcgg aaaccgaagg ataacttctt
      481 tcgaaaccgg gctgtcgaga cggcgcgtga atgtaccaat agccgttacg ggcgcagcga
      541 atagcgcact gctgcccgaa agaacagcga caagtatggc aaggcgcaga ttcatcttca
      601 gcgcagagga ttgtctgctt acttgcctgc ctgcatcgtg ctgatcatac tgccgatgac
      661 gctcttcagg tcatctttgc ggctgacatt ataattttgc acataaacga gctgcatcgt
      721 atcaagcttg atgacacgcg cccatgtata accggcatcc caccgtgaaa gcgagaggag
      781 tatgacgtag cggatgccga gttttttctg cagtccctgc agcatctcga gcttgaggtt
      841 ctcaaagttg aagatgtgta gtttgtagat attctcaaaa actttgtcgg caacggcaac
      901 ctggcgctgc gagtttttcg ggtcaaacag gttcgtgacg aaggcggtct ttttcagcga
      961 gcgcacatcg agcacgtcat ccgtcgtata gaagtcggtt gcattgaatg cctttacatc
     1021 gtaaccgccg cgcatgaact ccatgaacac ggcattcttg aagtcgtttt ccccttcgac
     1081 caggactgcc acattggagt tcttgggaat gggttctttg atcacgtagt tgttcgcggc
     1141 agatcgacaa ccgggcaggg taagcgcggt agccagggca accgccagaa aaacggacgc
     1201 ttttgtcatg tattacgccc gtccgatttc gtatcacccg tcaagagata ataggggtcg
     1261 cgggcaggtc acccccagcg accgccccgg gtagccttac agctgcgaaa tcatgttttc
     1321 ccagaagctg gtcgcagcaa agcgtacgtt gaggtcatac gaatcggcga tggtctccgc
     1381 cacatccgcc agcgtcgagc gtatgcccag gttggcctgc cgcctgggcc ggaacatgcg
     1441 cgagtagacc agcagcggaa catactcgcg ggtatggctg gtgccctcaa aagtggggtc
     1501 attgccgtgg tccgaaacga tatagagcat gtcctcattt tccattgcgc ggaagatacg
     1561 cggcaggtag gcgtcgagcg cctcgagcgc atcgatgtag cccatcgcgt cgcggttgtg
     1621 cgcgtagagg ctgtcgaggt ccggcagggt agcgacgata aatgactgct tgttgcggtt
     1681 cgtggtacct tcgcgtattg cctgcgtcag tatctcgagg cagtctgcat tgttcttcac
     1741 tgcatactgt tcagtgatgg ctgcaccctc gagcatttcg gatactttcc cgattgcatg
     1801 cacgggtata cctgcgtgcg ccaggttcgt ggcgagcgtg ggagcgcgtg gcggcgagtg
     1861 gaacatgccc gttggcattg tttcgtcagg ggcaaaagtc gcgaggtcgc cggtaaaata
     1921 gtgcaggtgc aggcggccca gcccgtgccg cgagagtacc tcccacgcgg aatgcccgag
     1981 caggtaaaac tcgtcgggct ggaacttgtc ggggtgcaca tagatattga tcagcgagtc
     2041 gtcggtgacc agaagaatgg gagcgcccgt gtccaggtgc gtttcgccgt attttgtcag
     2101 aaatccctgt tggacgtcgt tggcattgcc caggaaagaa cggccagtga tcgccgagag
     2161 ttcgtcgaga atttcgggcg gcaacccgcg gggaaacgta tggtattttt cttcctggta
     2221 gatgccaaga agttcacggt ggccaccgat gctgtcgttg ccctcattgc gctgcgttac
     2281 cttcgcgtaa aaaccgatcg tgtcttcttc gcgcggccac gatttcaggt atgtcaggtt
     2341 ggtgattccc atgcgcgaca gattgggaat ctgcagcggt gctttgagct cggcgatgtg
     2401 cgagagggtg ttggccccgc ggggaccata gaggtacgca tcgggctgtt cgcccacacc
     2461 gagcccgtca atgacgagca caattgatct gccaataccc agcgacaccc ggggcgatct
     2521 cgcgcgcaag actctgcccg gctataaatt tatgccaact tgcttgccaa tggcgcaaac
     2581 tctgccagtc tgcgcgatgc ctgccagccg gtttttgccg cccaccctgc tattcgccgc
     2641 atttttcacc gcgtgtcagc cgcagaagcg cctcgaaatt gcacacgcgc agggcaagaa
     2701 gcaggtttcc ctttccgagc tccgccagcg tgtcagcgac cgcgaagttg aggttgatga
     2761 ccattacctg caacgccgca ccagctaccg cgcgctgccg ctggcgccgc tcttgcgcgc
     2821 ctgggcccct gccgggcctt atgacgaagt gcttttccat tgcgccgatg gttatgaggc
     2881 gcgcgcgagc agggccgacc tcgagcaggg tcgactcgac agcttctttc tcgcctacgg
     2941 tgagggcggg gagcgttttc gttcgatgat acagcagggc aagtcgcagg tttcgcccga
     3001 accgttctac gcggtttcga ccgacccgaa aggttatgcg acgctcagct ggccgtacga
     3061 aattgtgggg gtggagttta ttcgttttgc tgacaaatac cccgggcttt tacccgacgc
     3121 cgtggcgcag aatgctgtaa cgcgcgcggg ctttgacctg tttcgcaaag aatgcctgcg
     3181 ctgccattcg ctcaacctga tgggcggcga aatcgggcca gagctcaacg tgccgcgcaa
     3241 catcaccgag taccgcgatg acgcttacct gcgggcattc atccgcaatg cctcggcttt
     3301 ccgcgcgcgc tcgaagatgc cgccgtttac gcagctaacg gatgttgagc tcgggaaggt
     3361 aatcgggtac ttgaaagtta tggcgcgctt taaggtccct aactagctgc gcaggcgcag
     3421 ctggttaggg accaatatac ttcttcttcg ccgcgttgtg ctcgcggaag tctttcgaga
     3481 agtggtgcgc accgtcaccc ttgagcacga agaacttgta gtcagtcttc tcggggttca
     3541 ctgtcgcatc gagcgcagcg atacccggtg agccgatcgg gcctgggggc aggccgcgta
     3601 ccttgtaggt gttatatggt gattcgactt cgaggtcttt ataaaacagc cgcgccttgg
     3661 gtttttcgaa caggtactgc accgtcgcac acgattcgag cggttcactg tcacgcatgc
     3721 ggttgtggaa taccgccgcg atcagcgggc gctcttcttt cagcttggct tcgcgttcta
     3781 caatactcgc gagaatcacg gtctggtgaa tcttttgcgg ctctgtcggg aagtttttca
     3841 cctctttgac tttctcgtag aaatgttcta ccatgtgctc gataattttg tccttggggt
     3901 aaccctgggg caagtggtag gtgtcgggaa aaatataacc ctctacggtc tgcgccggaa
     3961 tcttgaattt gtcgagtatc gctttcttcg aagcatactg cagaaattca tcgcgcgatg
     4021 cgaaaaactt tttctccacg aacaggtcgg cgatctggcg gttgtgcaga ccctcagcga
     4081 gtgtaatact gatcgtcttg gttttgccgc tcgtgatcgc ctgcgcaatc ttgccggcag
     4141 acatgccgtc gttcaggtca tagacgccaa acttgatgcg cgctgctttg cccgtcaggc
     4201 gcatgtacat cttaaagaaa aactggctgt ggatgagatt cgcctcagcg agccgcgagg
     4261 aaacagatga taaatttgaa ccggctttca cggcgaaatc gacatggcgc gagccatctg
     4321 agacgctgcg cagcgaaagc cagagcattg ccagtaagag aagcgtgacg gcacccaggc
     4381 cgccgccgcc aaacaggagc agtttgcgga agttttgttt gttgtcttgc agcataggtg
     4441 gtgcgttctg cctcctgccg gcaagtgatt gacatgctgt aaagcggcaa cgggagctga
     4501 acagtctcca tgcaaaaact cctctcgccg cttgtgctca tactgacgct ggtctttcac
     4561 tgccgctctt cgggcagcgc cgatctcgcg cgctatgccg gtaaaggggc aaacagcgag
     4621 aatggttacc ttaccttcta cgcgcagggg cagaagctga gcgcccgcac gcaggcaacc
     4681 ggcaaactac gggcatacct gctggcggcg atgcgcgagc cagatctgaa gctctcctgt
     4741 tcggcgtggg actgcgttgc cgctgcgctt tcgtcgatga gtagctctga tcgttcaccg
     4801 ctctacgcct acgcggttta ccacctggcg gcttttgccg agcagcagaa ctatccgcgc
     4861 gaggcatcgg aaatactcgc ccaggtaggt
//
LOCUS       DAOQJF010000259         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10268_length_4868_cov_5.707459, whole genome shotgun sequence.
ACCESSION   DAOQJF010000259 DAOQJF010000000
VERSION     DAOQJF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10268_length_4868_cov_5.707459"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLF85_12630"
     CDS             complement(<1..707)
                     /locus_tag="PLF85_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNE20563.1"
                     /translation="MNSPAATPSSAAQADPLQLGNLCLESRLIVGTGRYASLDEMRRC
                     HQVAETQMVTVAMRRYEGGASLGAKPAAASANSLLDFIDLDRIALLPNTSGAAFASDA
                     LRQARIAAAMGNTHLKIEVTGDIQTLLPDPLETLKSVEAIKAHADTRHLYLMVYTTDD
                     PVMARRLADAGAECVMPAASPIGSGRGIANEANLTILLTMMRGRVPVIVDAGIGTASH
                     AARAMELGADAVLLNTA"
     gene            complement(704..943)
                     /locus_tag="PLF85_12635"
     CDS             complement(704..943)
                     /locus_tag="PLF85_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20564.1"
                     /translation="MKDIEIAKILYLAKSKKYEVACASFDVVDHLFKLEIPKGLKRRK
                     SAVQTLSMLADEKVRYGYDRKDNVIVADDDEKEND"
     gene            1001..2449
                     /locus_tag="PLF85_12640"
     CDS             1001..2449
                     /locus_tag="PLF85_12640"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE20565.1"
                     /translation="MKNSPLIKHVYRLLFGHWPGENLPVTGRLVLFPKRLPEHLDAIY
                     FEEYIRALRMPVIWATILVFAIILIVICFYTSSFKYINEWFALALTGGAFTVLEIFWF
                     WDISRRWGYRTQLTILNVTLGVLVSILSWGIIGAWETMPANYIGMMTTIIGAILGAAA
                     VILSHSRVSFLVFMFSWIVPGIIFMFSKDDLFFHLMGWLFVSGIFSCMIINTMEYAKQ
                     RQLFDARQKLTEFNAELTRKDAELDQELVFASHIQMGIFPETKVNAGPYTFQTTILPL
                     GRISGDYCDIVTRGDITYAIVADASGHGVPAAMLTMAAKNVFSNLLTDRVSTASVMAR
                     ANHEILKMIKTQDYMTGFLLKLYPSGLVEFTNAAHPTAIFQSRQGDMRMLDTSGFILG
                     GLDDVDALYEGGSVQMQKGDRIFLYTDGVVECSNPDEHFYGVDRLEKMLRITRGLELS
                     RVTTRIFSDMVTFCEGVPFRDDIAMMVVEFNP"
     gene            complement(2446..3678)
                     /locus_tag="PLF85_12645"
     CDS             complement(2446..3678)
                     /locus_tag="PLF85_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20566.1"
                     /translation="MIARYVAAVIAATWLLWQIPFSHSESVIIPEVFATTQLAGLAET
                     EGNPALFFYRKTEAGQYAAYGLTPAGSIARVLNLPGSTLTKADQSQVSLGGKEGYLTG
                     PEDGAFFIWYPQLGSQVYVFNESGSLLWEKEESRYLQVLRHGRFILAAAGDHSRMVFM
                     NPDFKVQADFQGVLFTQFSADDDAGLKYGQACLGSLDGEVIVLHLDRRIYSRQRLGYA
                     LKALSCNFATGDMAAIVERTVSTDGKNRQADFLLRLKFNVLAGDDNAPAETLRQTTQV
                     AEVVSESEMPVRTVTASAITVTPDIICFVQAAPADGNETAAALYFTRYRKDKPQMVPL
                     GPARSAADSSTHDRWKSATVSLGTETGCLHAHASGRLVLGSARGLLFDRHDLGGERLL
                     VTGKSVFLQKADRVIGLR"
     gene            complement(3675..>4868)
                     /locus_tag="PLF85_12650"
     CDS             complement(3675..>4868)
                     /locus_tag="PLF85_12650"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE20567.1"
                     /translation="DFVVRDYQDASIRAFYADGSVEGGSGVVVLPCGAGKTIVGIGAM
                     HAVGAQTLILVTNTLSIRQWKNEILDKTTIPESEIGEYSGDKKEIKPITIATYNILTH
                     RKKKGGDFTHFHLFSSNNWGFIVYDEVHLLPAPVFRMTSELQAKRRLGLTATLVREDG
                     LEEDVFSLIGPKKYDVPWKELEKKKWIAEARCVEIRVPLRSLMRGMYSVADDREKFRL
                     SSENPEKQRVIGTLLYHHRNDQVLIIGQYLDQLQRVSARFKIPLITGKTPLEERAHLY
                     EAFRTKAISALVVSKVANFSIDLPDANVAIQISGTFGSRQEEAQRLGRVLRPKPNGNQ
                     ALFYSVITNESSEEQFCRNRQLFLTEQGYEYYIYSYQDFRDKYGTYIDVMKKMKEERE
                     AAVAK"
BASE COUNT          957 a         1369 c         1420 g         1122 t
ORIGIN      
        1 atcgccgtat tgagcagcac ggcatcggct cctagttcca tggcacgcgc ggcgtgcgaa
       61 gcggtgccga tacccgcatc gacgatgacc ggcacgcggc cgcgcatcat tgttagcaga
      121 atagtgaggt tggcttcgtt ggcaatgccg cgcccgctgc cgatcgggct cgcggcgggc
      181 atgacgcact cggcgccggc gtcggcgagc cggcgcgcca tcacggggtc gtcggtcgta
      241 tagaccatca ggtagaggtg gcgggtatcg gcatgcgcct tgatcgcctc gaccgactta
      301 agggtttcga gcgggtcggg caatagcgtc tgtatgtcgc cggtcacttc aatcttgagg
      361 tgcgtgttgc ccatcgcggc ggcgatgcgt gcctggcgca atgcgtcgga ggcaaaagcc
      421 gcccccgatg tgttgggtaa aagtgcaatg cggtccaggt cgatgaaatc gagcagcgaa
      481 tttgccgaag cggccgcagg tttagcccca agcgaagctc cgccttcgta ccgccgcatc
      541 gccacggtga ccatctgcgt ttcggcgacc tggtggcagc gccgcatctc gtcgaggctc
      601 gcgtagcgtc ccgtaccgac gattagcctg gattcgaggc ataaattgcc tagttggagt
      661 ggatcagctt gcgccgcgga actgggtgtg gcggcagggg aattcagtcg ttttcttttt
      721 cgtcatcgtc agcgacaatg acgttgtctt tgcgatcgta accgtagcgt actttctcgt
      781 cggccagcat gctgagcgtc tgcacagcgc ttttgcggcg cttcaggccc ttcgggatct
      841 cgagtttgaa caggtgatcg actacatcga agctggcgca cgccacctcg tattttttgc
      901 ttttcgcgag gtatagtatc ttggcaatct cgatgtcttt catacgcagg ttttggggaa
      961 tctcacccta ccatttttcc catacctgct gtaaacaaaa atgaagaatt cgcccctcat
     1021 caagcacgtc taccggctgt tattcggcca ttggccgggc gagaacctgc cggtcacggg
     1081 ccgcctggtc cttttcccga aacggctgcc cgagcacctg gacgcgatct actttgaaga
     1141 gtacatcagg gcgctccgta tgcccgttat ctgggccacg atactggtgt ttgccatcat
     1201 actgattgtt atctgcttct atacctcgtc tttcaaatat atcaacgagt ggtttgcgct
     1261 ggcgctgacc gggggagctt tcaccgtact ggaaatcttc tggttctggg atatcagccg
     1321 ccgctggggc taccgcacac agctcaccat attgaatgtc actctcgggg tgctggtgag
     1381 catactttcc tggggcatca tcggcgcctg ggaaactatg ccggctaact acatcggcat
     1441 gatgacgacg atcatcggcg cgattctcgg tgcggcggcc gtcatactgt cgcattcacg
     1501 ggtaagcttt ctggttttca tgttctcgtg gattgtaccg ggtatcatct tcatgtttag
     1561 taaagacgac ctgttttttc acctgatggg ctggttgttc gtcagcggta tcttctcttg
     1621 catgatcatc aataccatgg aatatgcgaa acagcggcag ctgttcgatg cgcggcagaa
     1681 attgactgaa tttaatgccg agctcacgcg caaagacgcc gagctcgacc aggaactcgt
     1741 gttcgcgtcg catatccaga tgggaatttt cccggaaacc aaggtcaatg ccggcccgta
     1801 cacattccag acgacgattc tgccgctcgg gcgtatttcc ggcgactact gcgacattgt
     1861 tacccgcggt gatatcacct acgcgatcgt tgccgatgct tcggggcacg gtgtgccggc
     1921 agccatgctg acgatggcgg caaaaaatgt tttcagcaac ctgcttaccg accgcgtttc
     1981 gacggcgtcg gtgatggcgc gcgccaacca tgaaatcctg aaaatgatca agacgcagga
     2041 ctacatgacg ggcttcctgc taaagctgta cccgtcaggt cttgtcgaat tcaccaacgc
     2101 tgcacacccg actgcgatct tccagtccag gcagggtgac atgcgcatgc tcgatacctc
     2161 aggctttatt ctcggcggac tcgacgacgt cgacgcgctg tacgaaggtg gcagtgtgca
     2221 gatgcaaaag ggcgaccgga tctttcttta taccgacggc gtcgtcgagt gctcaaaccc
     2281 cgacgaacat ttttacggcg tcgaccgcct cgaaaaaatg ctgcgcatca cgcgcgggct
     2341 tgaactgtcc cgcgtgacta cgcgtatctt ttccgacatg gtaactttct gcgagggcgt
     2401 accctttcgc gacgacatcg cgatgatggt tgtggagttt aacccctagc gcaggccaat
     2461 cacccggtct gctttctgca gaaagaccga cttgcccgtg acaagcaggc gctccccacc
     2521 caggtcatgc cgatcaaaca gcaggccgcg ggcgctgccg agcaccagcc ggcctgacgc
     2581 atgcgcgtgc aggcagcccg tttctgtgcc gagcgagacg gtcgcacttt tccagcggtc
     2641 gtgcgttgag ctgtcagcgg cagagcgggc cgggcccagc ggcaccatct gcggtttgtc
     2701 tttgcggtaa cgggtgaagt acagtgcggc tgcagtttca ttgccgtcag caggtgccgc
     2761 ctggacaaag cagataatat cgggagttac ggtaatagcc gaggcggtaa ccgtgcgcac
     2821 gggcatttct gattcagaaa caacttcagc cacctgtgtt gtctgtcgca atgtttctgc
     2881 cggggcattg tcatcgccgg ccaacacatt aaattttagc cgcagaagga aatctgcctg
     2941 gcggttcttg ccgtctgtgc tcacagtccg ctcgacaatg gccgccatgt cacccgtggc
     3001 aaaattacac gacagcgcct tgagcgcata accaagccgt tgccgcgaat agattctgcg
     3061 gtcgaggtgc agtacgatga cttcgccatc gagcgagccg aggcaggcct ggccatattt
     3121 caggccggca tcatcgtcag cactgaattg ggtgaagaga acaccctgga aatcggcctg
     3181 caccttgaag tcggggttca tgaagaccat gcgcgagtgg tcgcccgcag ctgcgagaat
     3241 gaagcggccg tggcgcagga cctgcaaata gcggctttct tctttttccc acagcaggct
     3301 gccgctttcg ttgaagacgt aaacctggct gcccagctgc gggtaccaga tgaaaaatgc
     3361 gccatcttcg ggaccagtca ggtaaccttc tttaccgccg aggctgacct ggctctggtc
     3421 tgccttcgtg agcgtgcttc ccggaaggtt aagtacccgc gcgatgctgc cagcaggagt
     3481 cagtccgtac gcagcgtact ggcctgcttc ggtcttgcgg taaaagaaca gggccgggtt
     3541 gccctctgtc tctgccaggc cggcgagttg cgttgtggcg aatacttcgg gaataatcac
     3601 cgactcgctg tgcgagaagg gtatctgcca gagcaaccag gtggcagcaa tgacggctgc
     3661 gacgtaacgc gcgatcattt tgctacggca gcttcacgct cttctttcat cttcttcatg
     3721 acgtcgatgt acgtgccgta cttgtcacgg aagtcctggt acgagtagat atagtattcg
     3781 taaccttgct cggtcaggaa gagctggcgg ttacggcaga actgttcttc gctcgactcg
     3841 ttggtaatca ccgaatagaa cagcgcctgg ttcccgttgg gtttagggcg cagaacacgg
     3901 ccgagtcgct gcgcttcttc ctggcggctg ccgaaagtac ctgagatctg tatcgcgacg
     3961 tttgcatcgg gaaggtcgat ggaaaagttt gcaaccttgg aaaccaccag cgcgctgatt
     4021 gccttggtac gaaaggcttc gtaaaggtgc gcacgctctt caagcggcgt cttgcctgta
     4081 ataagaggta tcttaaagcg tgccgagaca cgctgcagct ggtcgaggta ctgcccgata
     4141 atcagcacct ggtcgttgcg gtggtggtag agcagggtac cgataacgcg ctgcttctcg
     4201 ggattttctg acgagaggcg aaatttttcg cggtcgtcgg cgactgaata cataccgcgc
     4261 atcagcgagc gcagcggtac gcgtatttcg acgcaacgcg cttcggcgat ccactttttc
     4321 ttttcgagct ctttccaggg cacgtcgtat tttttgggcc cgatcagcga aaagacgtct
     4381 tcttcgagac cgtcttcgcg cacgagcgtt gcagtgaggc ccagccggcg ctttgcctgc
     4441 agctcactcg tcatacgaaa gaccggcgcc ggcagtaggt gaacctcgtc atagacgatg
     4501 aaaccccagt tgttggacga gaagagatgg aagtgagtga aatccccgcc ctttttcttg
     4561 cggtgcgtga ggatgttgta cgtggcgatc gtgatgggtt tgatttcttt cttgtcgccc
     4621 gagtactcgc cgatctccga ttcgggaatc gtggtcttgt cgagaatttc gtttttccac
     4681 tggcgaatcg aaagggtgtt ggtcacgaga atcagcgtct gcgctcccac cgcgtgcatg
     4741 gccccgatac cgacgatcgt cttgccggca ccgcagggta gcaccacaac gcccgaaccg
     4801 ccctcgacgc tgccgtcggc atagaaggcg cgaatcgacg cgtcctggta gtcgcgtacc
     4861 acgaagtc
//
LOCUS       DAOQJF010000260         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10269_length_4868_cov_4.290879, whole genome shotgun sequence.
ACCESSION   DAOQJF010000260 DAOQJF010000000
VERSION     DAOQJF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10269_length_4868_cov_4.290879"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..268)
                     /locus_tag="PLF85_12655"
     CDS             complement(74..268)
                     /locus_tag="PLF85_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNE20568.1"
                     /translation="MRTNVVLDEKLVKEAMKLTGKKTKRELIDYALHELVKRQKRLAL
                     LDLRGKLKWEGDLDAMKATR"
     gene            complement(326..1282)
                     /gene="sigJ"
                     /locus_tag="PLF85_12660"
     CDS             complement(326..1282)
                     /gene="sigJ"
                     /locus_tag="PLF85_12660"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HNE20569.1"
                     /translation="MSTSHLQLYTETRPRLLSLAYRMTGSLSDSEDILQDAWLRFAQV
                     PAEKIGSAAALLTTIVTRLCLDLMRSARKKRELYVGPWLPEPLPDLYLAHEDVEQRET
                     VSYAFLLLLEKLNPIERAVFVLREIFDYDYAEIATIVRKNGEHCRQIFHRAKNALRKE
                     YTARSTPGAREQQLLSAFLAACTGNDLQQLVAMLRDDAVMLNDGGGKVTASSIPIRGA
                     SRIAKFIFAVRDRDGKKSYYALRLNNTFGVLSYSEETPYALQLFEFGANDLQGSYVIR
                     NPDKLQYFSDRQALLRAGIIKPLARFMGLKATLVMLWRQLWR"
     gene            complement(1279..1902)
                     /locus_tag="PLF85_12665"
     CDS             complement(1279..1902)
                     /locus_tag="PLF85_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20570.1"
                     /translation="MPALPRQVGMLKKIHRTSAVVLLAFTTAHLANHLLALQGIEAHI
                     ALMKQLRACYRHPFAEGLLLIAVLAQVTTGSLLFWRTRRSLRGYWPRLRAYSGLAIAF
                     FLLQHVPAVLFGRLLQGLDTNFYFAAAVLQGGIHKYYFLPYYLVGVTSVFVHLAAVVY
                     LRLPGVRRRAWWSAALMVTGLVLALAIVLCFAGAFYEIRLPDGYGFP"
     gene            1984..2229
                     /locus_tag="PLF85_12670"
     CDS             1984..2229
                     /locus_tag="PLF85_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20571.1"
                     /translation="MKTTMILPDQLLQKAKLHAQKNHTTMTRLVEDALRAYLAAESNA
                     TPKKKWRIEPVGKGGFVSPDLEANWPKMRELLYEREK"
     gene            2226..2657
                     /locus_tag="PLF85_12675"
     CDS             2226..2657
                     /locus_tag="PLF85_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE20572.1"
                     /translation="MKAIDTNILVYAHRADNVWHARAKEFIELALTGEEKIGIPYHAL
                     VEFYGIVTNTRIFKNPTAPEVAIKQCKNLIAAPAAEILTESNASFEILAPVIAKSRVI
                     GSAVHDARIAAVCLEYGVRLVYTLDRDFSRFAGLKVQNPLV"
     gene            complement(3006..3215)
                     /locus_tag="PLF85_12680"
     CDS             complement(3006..3215)
                     /locus_tag="PLF85_12680"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HNE20573.1"
                     /translation="MTDSLLKQAMALDNLEKARLISMLNESLLSNEQKAIESNWATES
                     QKRYEDYKAGNHATVDYADIKRKGM"
     gene            complement(3301..4281)
                     /locus_tag="PLF85_12685"
     CDS             complement(3301..4281)
                     /locus_tag="PLF85_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE20574.1"
                     /translation="MVASEAYLSAEANFFDQYQKLPRFITEAFETAFFRSFLFAAENV
                     SGLRTKRTHAGGFESLYLEGGNPNGETLVWLHGFSDSRHSFALSSFWLRQKYHLIVPD
                     VPGFEERTYREHHTYNMEYYGEWFREFCAKLGLKNFYLAGNSLGGGIALQHAVHYPED
                     VKAVAAVNSAGVVGDEITAVYEEVQQGNNLFLVSDWPDFNRFLRRIYHNLVAVPFPIK
                     AFQYRRFRRYAGHFHKVMQDLTHGFLNSDADLAKDVINQYSLDHKITKLQRPTLVLWG
                     REDSLFPEMFARLTHANIPNAQLEFIEQCGHCPHLERPKEFALHLQRFFG"
     gene            complement(4369..4683)
                     /locus_tag="PLF85_12690"
     CDS             complement(4369..4683)
                     /locus_tag="PLF85_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE20575.1"
                     /translation="MGFMYILECCDGSLYTGSTKDLKTRLAHHEAGDGANYTKKRLPV
                     TLIYAEEYDRIDHAFAREKQIQGWRKTKKLALIDGNLSDLNLLAKCRNVTFYKLRENE
                     KA"
BASE COUNT         1136 a         1298 c         1346 g         1088 t
ORIGIN      
        1 gtcactctgt ggagaatgac tgcccagaaa gtggtcaatc cagactgagc tgtcgacaag
       61 tacgcttttc acttcagcgg gtggccttca ttgcgtcgag atcaccttcc catttgagtt
      121 tgccccgcaa gtcgagcagc gcgaggcgct tttgtcgttt caccagttcg tgcagggcat
      181 agtcgatgag ttcgcgcttc gtcttcttac ccgtcagttt catggcttct ttcaccagct
      241 tttcatcgag taccacgttc gtgcgcatac acataaagta gaaaaaatat acacatagtc
      301 aagatttaat catgacccaa tgcatttacc gccacagctg ccgccagagc atcaccagcg
      361 tcgccttcag tcccatgaac cgcgccagcg gtttgataat gcccgcacgc agcagtgcct
      421 gccggtcgct gaagtactgc agcttgtcgg ggtttctgat cacatacgaa ccctgcaggt
      481 catttgcgcc gaattcaaac agctgcagcg catagggagt ttcttcgctg tagctcaaca
      541 ccccgaaggt gttgttgagc cgcaatgcat aataagactt tttgccgtcg cggtcacgca
      601 ccgcgaagat aaatttcgcg atgcggctgg cgccgcgaat cggtatcgat gaggctgtca
      661 ctttgccgcc gccgtcgttc agcatgactg cgtcgtcgcg cagcattgcc acgagctgct
      721 gcagatcgtt acccgtgcag gcagccagaa aagcagaaag cagctgctgt tcacgcgcgc
      781 cgggtgtcga gcgcgcagta tattctttgc gcagggcatt cttcgcccgg tgaaaaatct
      841 gccggcagtg ctcgccattc ttgcgcacga tggtggcgat ctctgcgtag tcgtagtcga
      901 agatctcgcg cagcacgaag accgcgcgct cgatgggatt tagtttctcc agtaaaagaa
      961 gaaacgcata actcactgtt tcgcgttgtt ctacatcttc gtgcgcaagg tagagatcgg
     1021 gcagtggttc gggcaaccat ggcccgacat agagctcgcg cttcttccgc gcgctgcgca
     1081 tcaggtcgag gcagagccgc gtgacaatgg ttgtgagtag cgcagccgct gaaccgattt
     1141 tttctgccgg aacctgcgcg aagcgcagcc aggcatcttg cagaatgtct tcgctgtcgc
     1201 tgaggctgcc cgtcatgcgg taggcgagcg aaagcaggcg cgggcgcgtt tcggtgtaga
     1261 gttgcaggtg gcttgtgctc atggaaaacc gtagccgtcc ggcaggcgga tttcgtagaa
     1321 tgcaccggcg aagcaaagca ctattgccag cgcgagtact aaccccgtca ccatgagtgc
     1381 agctgaccac cacgcgcgtc gccgcacacc cggcagacga agatacacaa ccgcggcgag
     1441 gtgcacgaag accgaggtaa ctcccaccaa gtaataagga agaaagtagt acttgtgtat
     1501 gccaccctgc agaacagccg cggcgaaata gaagtttgta tcgaggccct gcagaagccg
     1561 gccaaaaagc accgctggca cgtgctgcag cagaaaaaat gcgatcgcca ggcccgagta
     1621 cgcgcgcaag cggggccagt agccacgcag gctgcggcgc gtgcgccaga agagcaggct
     1681 gccggtggtt acctgcgcca gcaccgcgat cagcagcaac ccttcagcga agggatggcg
     1741 gtaacaggcg cgcagctgct tcattagggc gatatgcgct tcaatgccct gcagcgcgag
     1801 caggtggttg gcaagatgcg cggtagtgaa agcgagtaga acaaccgcac tggttctgtg
     1861 aatttttttc agcataccta cctgacgggg cagggcgggc atttgtgaca gggagtgtga
     1921 ttttttctgg gccaaattgc gattctattt gccatatcat atggtagata atatattgca
     1981 catatgaaaa cgacgatgat tctgcccgac cagctgctgc aaaaggcgaa gttgcacgcg
     2041 cagaaaaatc atacgacgat gacccgactg gtggaagatg cactgcgagc ataccttgcg
     2101 gccgaaagta acgcgacccc gaagaaaaag tggcgcatcg aacctgttgg caaaggtggt
     2161 tttgtgtcgc cagaccttga agcgaattgg cctaaaatgc gagagctact ctacgagcgt
     2221 gaaaagtgaa ggcaatcgac accaacatac tggtctacgc ccaccgtgct gacaatgtat
     2281 ggcatgcgcg cgcgaaagaa tttatcgagt tagcgctcac gggtgaagaa aagatcggca
     2341 taccctacca tgcactcgtc gagttctatg gtatcgtaac gaacacacgc attttcaaga
     2401 atccgacggc gcccgaagtg gcaatcaaac aatgcaaaaa tcttatcgcc gcacccgcag
     2461 ccgaaattct cacggaatca aatgcgagct tcgagatact tgccccggtg atcgcaaaaa
     2521 gccgtgtgat tggctcagca gtgcacgacg cgcgcatcgc ggcagtttgt ctcgagtatg
     2581 gtgtgcggct ggtctacacg ctcgaccgtg atttcagtcg gttcgccggg ctgaaggtgc
     2641 agaatccgct ggtgtagtat tttgcgtcag cagaaacagc gcatctgagc aaactccaag
     2701 gcttcgccca cttcggctct caggtggcac taatttcgcg aaaatgtttc aggattgcga
     2761 tggagattca tcacagacaa gaccaaaact atttcaggtt cggtcgcata caatatgccg
     2821 taaggaaaat ggtcgattag acagcggcgc acatcttttg ccactaattg ccaggcgtgc
     2881 gggaatcctg gccaaggtct tatcgaattc ttcggcaaaa tagaaaccca agccggactt
     2941 ctgacgctcg tagtaatcta ctgcttgatt atattctgaa gcagcccttg caagaaaacg
     3001 aattctcaca tgcctttgcg cttaatgtca gcataatcga ccgttgcatg attgccagct
     3061 ttgtaatcct cgtaacgctt ttgtgattca gtcgcccagt tgctttctat agccttttgt
     3121 tcattgctca gcagcgattc attcaacatg ctgatgagcc tggccttttc cagattgtcc
     3181 agggccatgg cctgcttgag caaagaatcg gtcatggcaa aaaataccga actgacctca
     3241 gacgtcaaca atctactgaa tttctctgat ttcgcgacta ttagccgacg taatgccacc
     3301 tcacccaaag aagcgctgca ggtgcagcgc gaattctttg ggtcgctcaa ggtgcgggca
     3361 gtggccgcac tgttcaataa attcgagctg cgcgttgggt atgttcgcat gcgtcaggcg
     3421 cgcgaacatt tcaggaaaca gcgagtcttc gcggccccac agcacgagcg tcgggcgctg
     3481 cagttttgtg atcttgtggt cgagcgagta ctggttgatc acgtctttcg cgagatcggc
     3541 gtcgctgttc aagaagccat gcgtcaggtc ttgcatgacc ttgtgaaagt ggccagcata
     3601 acggcgaaag cgccggtact ggaaagcctt gatcggaaaa ggcaccgcga cgaggttgtg
     3661 gtagatgcgg cgcaagaaac ggttgaagtc tggccaatcg gaaaccaaaa acaggttatt
     3721 gccctgctgc acctcttcat agaccgccgt gatttcgtcg ccgaccacgc cggcgctgtt
     3781 gaccgccgcc acggctttga catcttcggg gtagtgcacg gcgtgttgca gagcgatacc
     3841 tccgcctaag gaattgcccg ccaggtagaa gttctttaac ccgagttttg cacagaattc
     3901 acgaaaccat tcaccataat attccatgtt gtacgtgtgg tgttcgcggt acgtgcgctc
     3961 ttcgaagccg ggtacgtcgg gcacgatcaa gtggtatttc tgccgcagcc agaaagacga
     4021 gagcgcaaac gaatggcggc tgtcagagaa gccgtgcagc cacacgagcg tttcgccgtt
     4081 ggggttgccg ccttcgaggt agagcgattc gaagcccccg gcatgcgtgc gcttggttct
     4141 aagccccgaa acattttcag cggcaaacaa gaacgaacga aagaacgcgg tctcaaacgc
     4201 ttcggtgatg aacctgggca acttctggta ctggtcaaag aagttcgctt cggccgacag
     4261 gtatgcttcg gatgctacca tgtcccctga gacggaaaaa tgaacagggt gtcaaggtgt
     4321 aggaggggtg gatttttcgg attacgcata taggtcgcga gcgtggtatc aggccttttc
     4381 gttctcccgc aacttataga aagtgacatt tcgacacttt gccagcaggt ttaggtcaga
     4441 aaggttgcca tcgatcaacg ccagcttttt cgtctttcgc cacccttgta tctgtttctc
     4501 tcttgcgaat gcatggtcta tgcggtcata ttcctcggca tatatcagcg tcacgggcag
     4561 gcgctttttc gtatagttgg ctccatcgcc tgcttcatga tgggccagac gggtcttcag
     4621 atccttggtg ctcccagtgt acagagagcc gtcgcagcat tcgagaatgt acatgaaacc
     4681 catgggtcac ttcgctacgg tcacttcgac ttcgctccga agcggagacc ttgcggtcgc
     4741 agtgaccttg caatcactcc tgcacagaaa aagaagcctc gctcggtgag cggagtcgaa
     4801 ccgcgcgagg agggaatggt cgcttcgact ccgctccgaa gacggagacc ttgcggtcac
     4861 agcgacct
//
LOCUS       DAOQJF010000261         4788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10522_length_4788_cov_4.502852, whole genome shotgun sequence.
ACCESSION   DAOQJF010000261 DAOQJF010000000
VERSION     DAOQJF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10522_length_4788_cov_4.502852"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLF85_12695"
     CDS             <1..300
                     /locus_tag="PLF85_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE20576.1"
                     /translation="GGGLIMIPGLVLLLGIDQHTAIGTSLAVMLPPIGIAATYNYYKS
                     GSVNLAYAVVIALAFIVGSFLSSKLALTLPEMTMRRIFAVFLVIMGVRMFFGKGG"
     gene            complement(309..>2428)
                     /locus_tag="PLF85_12700"
     CDS             complement(309..>2428)
                     /locus_tag="PLF85_12700"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE20577.1"
                     /translation="MKTPLRTDANGIPHLEASDPKELFYQLGVAHAAQRSVQMLLMRI
                     LGEGRGSELLDNSDTMLGIDKFFRRMNWTRGLDAEVHKLSGAELAILNAYCDGINSVL
                     AAKLPWEFKLLGLKFEKWLPEHSILIARMMGYLTLSQSQAEVEKLFIELVQAGADKGR
                     LAELFPGLLAHADLSLIKEIDLEHRITPPEYLWQLGLPRMMASNNWVIAGSRTQSKKP
                     ILSNDPHLEGNRLPNVWHEFTWRCGDRSGAGAGVPGLPGMMVGRNDSVAWGATYTFMD
                     AVDSWIEKCRDEKFLDHDKKWKSFELRREVIKRKKKPDAEVVFYENKRGTLEGNASGS
                     DEKLMLTTAWSGAQAGAASIRGALAMPFAKSAKEGMQILGNLEVSFSWVLADNAGNIG
                     YQMSGLMPKRRNGWKGFVPVPAWKKNFDWQGFAKPAELPRRYNPKEGFIVTANNDLNS
                     WGKQKPINMPMGNYRARRIAQLLTASKKHTVAGVQQQHYDTYSLQAEEFMKILKPMLG
                     NSAAEQALAAWDMQYTPESFGAGIFEAWYRALYSEVFGATGKDAAQFMWKEGGIFIDF
                     YQNFDRILLAKKSAWFGSREDIYKAALERIKNLQPQPWGETNRFVLRHLLFGGKFPRF
                     MGFDHGPIPIRGGRATVHQGQIYRSAGRDTSFIPTFRFVTDFSERVLYSNLLGGPSDR
                     RFGKFYRNEVDNWLNGVYKKLEF"
     assembly_gap    2429..2528
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2529..3931)
                     /locus_tag="PLF85_12705"
     CDS             complement(<2529..3931)
                     /locus_tag="PLF85_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20578.1"
                     /translation="MRYVTLVCLLFSTLHCNSWGRFWEVSPAEPERSWYSYIGGPGLD
                     NAQNVRQAADGGYYTLSLSQAAFTNLGGKTPLNSYTGLSDFFITKLAKDGELQWYTFF
                     GTTADDWTHDLVPAADGGVLALGYTAGISSSIAGVAPIYQAVSGSYGLVAKFNANGGV
                     DWWTYFGGGGAQLRGAAATSDGGFIVVGFSNANFTNTSCTTPLVAHSGSQFDIFVAKL
                     SSTGVTQWCTFLGGSANDYGIQVQQAVDGGYFVGAHFDANTGTLAGVSPLSSLSTFQG
                     SQDMAMLKLTAGGSLSWWGYFGSVGADNLADFEVTADGGLVIAGMFGGNPGSIGSFTY
                     TGSYGGGDDAVILKMNSAGAIEWYRLYGGTGNDSLNGVAVSTDGGFIVSGRTGANITS
                     LYSLSPRRLYNAADDILLMRTDSAGTLQWYSFYGGTGTDGGAGIAQAKDGGFIFSGTA
                     AQEITDISTTAPLLPYNA"
     gene            complement(4046..4648)
                     /locus_tag="PLF85_12710"
     CDS             complement(4046..4648)
                     /locus_tag="PLF85_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNE20579.1"
                     /translation="MRFFLQALSVALALFPVSAESPWERKLHRDGIVVDVRSVADSDF
                     KEFKARMRVKSTLVKATEIMRDIPGYTRWMKDCKEAREVQKISASSGIVYSLQAAPWP
                     ITEREAVVRYDYRRSSTPAALYITIAAEPNALPQKSGRVRIPRLKGYWSYVEISAGEL
                     EVTYSMHSEPGGSLPGWAAAGMVAHLPYETMKRLKAELER"
BASE COUNT         1038 a         1357 c         1270 g         1023 t
ORIGIN      
        1 ggcgggggat taatcatgat tccgggcctc gtgctcttgc tgggtattga ccagcacact
       61 gcaattggta ccagcctcgc ggtgatgctg ccaccaatcg gcattgcggc gacctataac
      121 tattacaagt caggctcggt taacctggcg tacgcggtgg tcattgcact tgcctttatc
      181 gtcggtagct ttctttcatc aaagctcgcg cttacgttac ccgaaatgac gatgcggcgc
      241 atcttcgccg tgttcctggt catcatgggt gtgcggatgt tctttggcaa gggcgggtga
      301 aattcaattc aaaactccag cttcttatac accccgttca gccaattgtc cacttcgttg
      361 cggtaaaatt ttccaaaccg ccggtctgag gggccgccga gcaggttcga gtacagaacg
      421 cgctcgctga aatctgtaac gaagcggaat gtcggtataa aagaagtatc tcgtcctgca
      481 gagcggtaga tctgcccttg gtgcacagtc gcgcgcccgc cgcgaatcgg tatggggccg
      541 tggtcaaaac ccatgaagcg cggaaacttg ccaccgaaga gcaggtgccg cagcacgaag
      601 cggtttgttt cgccccacgg ttgtggctgc aaattcttga tacgctccaa cgccgctttg
      661 taaatatctt cgcgcgaacc aaaccaggct gactttttcg ccagtaaaat gcggtcgaag
      721 ttctggtaga agtcgatgaa gataccgccc tctttccaca tgaactgggc tgcatcttta
      781 cctgtggcgc cgaagacctc actgtaaagt gcacggtacc aggcttcgaa tatgccagcg
      841 ccgaagcttt cgggtgtgta ctgcatgtcc cacgcggcga gtgcctgctc tgctgcagag
      901 ttgcccagca tcggcttcag gatcttcatg aactcttcgg cctgcagcga ataggtgtca
      961 tagtgctgct gctgcacgcc cgcgacagtg tgtttcttcg acgccgtgag cagctgcgca
     1021 atgcggcgcg cccggtagtt acccatcggc atgttgattg gtttctgctt gccccagcta
     1081 ttgagatcat tgttcgccgt gacaatgaac ccttctttcg gattatagcg gcgcggcagc
     1141 tcggccggct tcgcaaaacc ctgccagtcg aagttcttct tccaggcggg tacaggcaca
     1201 aaacctttcc agccattgcg gcgtttgggc atcaggcccg acatctggta accgatgttg
     1261 ccggcattgt cagccagcac ccagctgaac gaaacctcga ggttaccgag aatctgcata
     1321 ccttctttag ctgacttcgc aaatggcatc gcgagcgcac cgcgaatcga agcggcacct
     1381 gcctgcgcac ccgaccatgc cgtcgtgagc atcagcttct catcactgcc cgaagcatta
     1441 ccttcaagcg tgccgcgctt gttctcataa aaaaccactt ctgcatctgg cttcttcttg
     1501 cgcttgatca cctcgcggcg gagctcaaac gatttccatt ttttgtcgtg gtcgagaaat
     1561 ttttcatcgc gacatttctc gatccatgag tcgactgcat ccatgaacgt gtacgtggca
     1621 ccccacgcga ccgaatcatt gcggccgacc atcatacccg gcaggccagg aactcccgcg
     1681 ccagcgcccg agcgatcgcc gcaacgccag gtgaactcgt gccatacatt tggcaggcgg
     1741 ttgccctcga ggtgcgggtc gttcgagaga atcggcttct tagactgcgt gcggctaccg
     1801 gcaatcaccc agttgtttga tgccatcatg cgcggcaggc cgagctgcca caggtattcc
     1861 gggggagtga tcctgtgttc taagtctatt tccttaatta aggaaagatc cgcgtgcgcg
     1921 agcaggcccg gaaaaagttc agccaggcgc cctttgtcag cacccgcctg caccagctcg
     1981 atgaagagtt tctccacctc agcctgcgac tgtgaaagtg tcaggtagcc catcatgcgc
     2041 gcaatgagaa ttgaatgctc gggtagccac ttctcgaact tcagacccag caatttgaat
     2101 tcccacggca gctttgccgc caagacagaa ttgatgccat cgcagtaagc gttgagaatc
     2161 gccagctcgg ctccgctcag cttgtgtacc tcggcatcaa ggccgcgcgt ccagttcatg
     2221 cggcgaaaga acttgtcgat gccgagcatc gtgtcgctgt tatcgagtag ctcagaaccg
     2281 cgaccctcgc ccagaatgcg catgagcagc atctgcacgc tgcgctgtgc cgcgtgcgcg
     2341 acaccgagct gatagaaaag ctctttgggg tcagaagcct cgaggtgagg tatgccgttg
     2401 gcgtcggtgc gaagtggtgt tttcatggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnngc attgtaaggt agcagcggag cagttgtgga aatatccgtg atttcctgcg
     2581 ctgccgtgcc tgaaaaaata aatccgccgt cttttgcctg cgcaatacct gcaccaccgt
     2641 cggtacccgt gccgccataa aatgaatacc attgcaaggt acccgcagaa tcggtgcgca
     2701 tgagcagaat gtcatctgct gcgttataca gcctgcgcgg tgaaagacta tagagcgaag
     2761 taatattcgc accggtcctg cccgagacta taaagccgcc gtcggtagag actgcgacgc
     2821 cgttgaggct gtcattgcct gtgccgccat acaaacggta ccactcgatt gcgcccgcag
     2881 aattcatctt gaggatgact gcatcatcgc caccgccgta tgatccggtg taggtgaatg
     2941 agccgatgct gccggggttg ccgccgaaca tgcccgcgat gaccaaacca ccatcggcag
     3001 tcacttcaaa gtcagcgaga ttgtctgcac caacactgcc gaagtacccc caccagctca
     3061 gtgagccacc ggccgttagc ttgagcatcg ccatatcctg gctgccttga aaggtcgata
     3121 gcgaactgag gggcgagaca cccgccaggg ttcctgtatt ggcgtcgaaa tgtgcaccga
     3181 caaaatagcc gccgtcgaca gcctgctgca cctgtatacc ataatcgttc gcagagccac
     3241 cgaggaatgt acaccactgc gtgacaccgg tcgaactgag cttcgccaca aagatatcaa
     3301 actgtgaacc tgaatgggca acgagcggtg tcgtgcagga agtgtttgtg aagttagcat
     3361 tggagaagcc cacaacgatg aaaccaccgt cagaagttgc cgcagccccg cgcagctgcg
     3421 cacccccacc gccaaagtaa gtccaccagt caacgccgcc gttggcattg aacttcgcca
     3481 ccaggccata actgcccgag acagcctggt atatcggcgc aacgccagca atgctgctgc
     3541 tgatgccagc tgtgtaacct aaagcaagaa caccgccgtc agccgcgggc acgagatcat
     3601 gcgtccagtc gtcagcggtt gttccaaaga aggtgtacca ctgcaattcg ccatcttttg
     3661 caagcttcgt aatgaagaaa tccgaaagcc ctgtgtagct gttcagtggc gtctttccgc
     3721 cgagattggt gaacgccgcc tgcgacagag aaagcgtgta gtagccgcca tcggccgctt
     3781 gccttacatt ctgcgcgtta tcgagccccg gcccgccgat gtagctgtac caactgcgct
     3841 cgggttcagc aggagagacc tcccagaatc ttccccagct gttacagtgc agcgtcgaga
     3901 agagcaggca cacaagggtg acatagcgca cgggatgtgc gcctcagtaa gcacattggg
     3961 tgtcaagcgc acgtgaagga aaattaatac atgaaatgat tatcctatat agaaaatgcc
     4021 tgggatgcgg cctggatatc ctgccttaac gctctagctc agccttgagc ctcttcatcg
     4081 tctcgtaagg caaatgtgcg accattcccg cagcagccca gccgggcaga ctgccgccgg
     4141 gttcggaatg catgctgtag gtcacctcga gctcgccagc cgaaatttcg acgtagctcc
     4201 agtagccttt caggcgcgga atgcgcacgc gcccactctt ctgcggcaag gcattcggct
     4261 cagcggcaat cgtaatatat aacgctgctg gtgtgctcga gcgccggtag tcatagcgta
     4321 cgactgcctc gcgctcggtt atgggccaag gtgccgcctg cagcgaatag acaataccgc
     4381 tagaggcaga aatcttctgc acttcgcgcg cttccttgca gtctttcatc cagcgcgtat
     4441 accccgggat atcacgcatg atttcggtgg ccttgaccag ggtcgatttg acacgcatgc
     4501 gtgccttaaa ttccttgaag tcggaatcag cgaccgaccg cacgtcgacg acgataccgt
     4561 cacggtggag cttacgctcc cagggtgatt ccgcggagac aggaaacaga gccagggcga
     4621 ccgacagggc ctgcaagaaa aagcgcatgg ctcatttatt ttcgttattt agccaatcaa
     4681 ctaattattg acgcctgaag ctgaattata ttgtaaaccc cgctagggat gcggaagtct
     4741 caatgggtgt cgatgctgaa gccgctgcgc gagaaaggcg gcgacctg
//
LOCUS       DAOQJF010000262         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10573_length_4773_cov_5.880246, whole genome shotgun sequence.
ACCESSION   DAOQJF010000262 DAOQJF010000000
VERSION     DAOQJF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10573_length_4773_cov_5.880246"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLF85_12715"
     CDS             <1..1209
                     /locus_tag="PLF85_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20580.1"
                     /translation="LQKHGFEAWLVGGSVRDLLIAGKLSDLDYTTNATPEQVQKIFPR
                     TVPVGIKFGTILVLYRGQKVEITTYRADADYHDGRRPSTVHYAEKLGTDIQRRDFTIN
                     GLAYNVSRAELADYCGGLEDLQAKVLRTIGDPLQRFAEDGLRPIRGCRIAAKLGFTIE
                     PATLTAMRQCIDITAKVAPERFFDEWRKTLRMKNRRSYWHNLLEAHILPAFLPHIAQA
                     FIGEPRDQFLREVDHLHLRSMAEYAAAVFYLLQIDDAKEREATLHETKFPTADLKLCL
                     SLLTSPLFTLPETPGRQEFKDTLALVKKAERFAHVRFFRAMRAASMVTQGVLPGPVRK
                     FRETTASLYATVRRAKEPLDISDLAVTGTEIQQAGFKGPAVGEQLEKLRRAVLQNPAL
                     NTKDQLLKLL"
     gene            complement(1249..1971)
                     /locus_tag="PLF85_12720"
     CDS             complement(1249..1971)
                     /locus_tag="PLF85_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE20581.1"
                     /translation="MMKKVGRWLGVTILKWAMELIYMTCRVTYVDGKEIRDEILARRR
                     PVIFMFWHNRIFMAAIYLKRTFFDKGVHLTVLISQSGDGEAIANVVERWGGDVARGSS
                     SRGGKAALTMLSHALSRRKSSVVMTPDGPRGPMYVFQAGALVASQLTQTELVPVCVSP
                     RRFWRLNSWDGFLIPKPFTELLVSVWPSETEARKQSEEEREQRRQYHEKRMLEQRLKL
                     DALANVPEYAGKRKKNQPATGS"
     gene            complement(2009..2527)
                     /gene="dcd"
                     /locus_tag="PLF85_12725"
     CDS             complement(2009..2527)
                     /gene="dcd"
                     /locus_tag="PLF85_12725"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNE20582.1"
                     /translation="MILSGIEIKKRLGKDIFIEPYDEKYLNPNSYNLTLHDELLVYKE
                     SLLDMKKPNETQLLKIPESGFVIEPGRLYLGRTRELTETKNLVPMLEGRSSVGRLGLF
                     VHITAGFGDIGFRGFWTLEISSIQPVRIYPGVQICQIFYHTIEGEFTEYKSGKYQGNT
                     GIQPSLIYKDFE"
     gene            complement(2524..3576)
                     /locus_tag="PLF85_12730"
     CDS             complement(2524..3576)
                     /locus_tag="PLF85_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNE20583.1"
                     /translation="MKQQLENLISRVQNPLTHKSIADDKLFIAIEDGEKPVLVLKAAG
                     DKKWQLAIEAQVRTLASKEGIAPDGFKFKWSDAKPEVTKQGPVAKPQRSIPGVKHVIA
                     IASGKGGVGKSTVSVNLASTLQQMGKKVGLMDADIYGPSVGKMLGLSGRQQIDVKNDR
                     ITPVEKYGLKLMSFSFLVDDNQPIVWRGPMLGKAVEQFLYDMNWGELDFLIIDLPPGT
                     GDTQLSLAQLVQTDGAVIVTTPQSVALLDAGRAVAMFEQVNIPILGVVENMSEFICSH
                     CGKPSHIFSKGGGSRLAASTDSVLLGQVPLVEDCMAAAEKGEIPVYKNKNKNIKPVVD
                     AFEHIARNLVSKLENA"
     gene            complement(3577..>4773)
                     /locus_tag="PLF85_12735"
     CDS             complement(3577..>4773)
                     /locus_tag="PLF85_12735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE20584.1"
                     /translation="SDQPVFSRTQWTVLGILAVIQFMHIVDFMVIMPLARKLEVEFHI
                     STNQFGWLVSSYTFAAFFSGIAGSFFIDRVSRKASLLIGSAGFIAGNLFCAVAPGFWY
                     FLAARVVTGAFGGLLSGVTFSIIGDLIEPHKRGRATGVIMMAFSVAAVVGVPAGIWFA
                     GRFFLQLPFYLIAAGSLAVLALTALRMPSLDAHLAGPRSQVIQQIRSVLADSKNRRLY
                     LMMVFHFVAGFSIIPFIANFMQKNNGVSDDQLFWIYVTGGLATIVSSPFAGTLTDKYG
                     PVRIYSFLSLVASLPFILVTYEWTKFFPLLIVFTVLFFVFVSSRMVPAMALLNNTVNP
                     ALRGTFMSLNGSVQQFAMSLGAMAASLIIVAPPGQPIQNYLWVGIFGIFFNIAAIAVA
                     NTFKGR"
BASE COUNT         1073 a         1316 c         1334 g         1050 t
ORIGIN      
        1 ctgcagaagc atggttttga agcctggctc gtgggcggca gcgtgcgcga tctgctgatc
       61 gcaggcaagt taagtgacct cgactatacg acgaatgcca cacccgaaca ggtgcagaag
      121 attttccccc gcactgtacc ggttggcatc aagttcggta cgattctcgt cctctatcgc
      181 gggcagaagg tagaaatcac gacctaccgc gcggatgctg actaccatga cgggcgccgg
      241 ccttcgacgg tgcactacgc tgaaaagctg ggtaccgaca tccagcggcg cgacttcacc
      301 atcaacggcc tggcctataa cgtcagccgc gccgagctgg cagattactg tggcggcctc
      361 gaagatttgc aggcgaaggt cctgcgcacg atcggtgatc ccctgcagcg ctttgcagaa
      421 gatggtttgc gcccgattcg cggttgtcgc atcgcggcga agctgggttt tacgatcgaa
      481 cccgccacgc ttaccgcgat gcgccagtgc atcgacataa cggcaaaagt agcgcctgaa
      541 cggttttttg acgaatggcg caaaacgctc cgcatgaaaa accgccgctc atactggcat
      601 aacctgctcg aggcacacat attaccggcc tttttgccgc acatcgcgca ggcctttatc
      661 ggcgagccgc gcgaccagtt tttacgtgaa gtggatcacc tgcacctgcg cagtatggca
      721 gaatatgcgg ccgcggtttt ttacctgttg cagattgacg atgcaaaaga gcgcgaggcg
      781 acactgcacg agacaaagtt tcccacggct gatctgaagc tctgcctctc tctgctgacg
      841 tcgccgcttt ttactctgcc cgaaactcct ggacgccagg aatttaagga cacactggcg
      901 ctggtcaaaa aggcggagcg tttcgcgcac gtccggtttt ttcgtgcgat gcgggccgcg
      961 agcatggtaa cgcagggtgt actaccgggg ccggtcagaa aatttcgtga aaccacggcg
     1021 tctctctatg ccacagtgcg cagggccaaa gagccgctgg acatatctga cctggcagtc
     1081 acaggcactg aaatacagca ggccggcttt aaggggcctg ctgtcggtga gcaactggag
     1141 aagctgcgcc gggctgtttt gcagaacccc gcgctcaata cgaaagatca gctgttgaaa
     1201 ttgctctagc cgtgtgcgcc aggcagcaca agtggcgcta tgcggctatc acgagcctgt
     1261 ggctggctga ttcttttttc gcttaccggc atactctggt acgttcgcca gtgcatcgag
     1321 cttgagtcgc tgttccagca ttcgcttctc atggtactgc cggcgctgct cacgctcctc
     1381 ttcactctgc ttacgagcct cggtctcaga aggccagaca gaaaccagca gctcggtaaa
     1441 gggttttggt atcagaaagc catcccagct gttgaggcgc cagaaacgtc ttggtgaaac
     1501 gcacactggt accagctcgg tttgtgtcag ctgcgatgca acaagcgcgc ccgcctgaaa
     1561 gacatacatc gggccgcgcg gaccatcggg tgtcatcacg accgaactct tgcggcggct
     1621 gagtgcatgg ctcagcatgg tcagcgccgc tttaccgcca cgcgacgacg aaccccgggc
     1681 aacatcgcct ccccagcgtt cgacaacgtt agcaatggct tctccatcgc ctgactggct
     1741 gatcagcacg gtcaggtgaa ctcccttgtc gaagaatgtg cgcttcaggt agatcgccgc
     1801 catgaagatg cggttatgcc agaacatgaa gatgaccggc cgcctgcggg caagaatttc
     1861 atcgcggatt tctttgccgt caacataggt tacgcggcag gtcatgtaga tgagttccat
     1921 tgcccatttg agaatggtga ccccgagcca gcgcccgact ttcttcatca tggttgagtt
     1981 attttcacgg cgttgccgtg aaaataaact attcgaaatc tttgtagatg aggcttggct
     2041 gtatgccggt attgccctgg tacttaccgc ttttgtattc agtaaactcg ccttcgattg
     2101 tatggtagaa gatctggcag atctgcacgc ccgggtagat gcgcacgggc tggatgctcg
     2161 aaatttccag cgtccagaag ccgcggaaac caatatcgcc gaagccggca gtgatgtgca
     2221 caaaaaggcc caggcgaccg acgctgctgc ggccctcaag catgggcacg aggttcttgg
     2281 tttctgtcag ttcacgcgtg cgaccgaggt acaggcggcc gggttcaatg acaaaccctg
     2341 attcgggtat cttgagcagc tgcgtctcgt ttggcttctt catgtcgagc agcgattctt
     2401 tgtataccag cagctcgtcg tgcagagtca gattataaga gttgggattg agatacttct
     2461 cgtcataggg ctcgatgaag atatctttgc cgaggcgctt ctttatttcg attcccgaga
     2521 ggatcatgcg ttttccagtt tagagacgag gttgcgggcg atgtgttcaa acgcatcgac
     2581 cacgggctta atgttcttgt tcttgttttt gtaaaccgga atctcgccct tctctgcggc
     2641 agccatgcag tcttcgacca gtggcacctg tcccagtaaa acactgtcgg tacttgctgc
     2701 cagtcgggac ccgccgccct tggaaaagat atggctgggc ttgccgcagt ggctgcagat
     2761 gaattcgctc atgttctcga ccacacccag tatggggatg ttaacctgtt caaacatggc
     2821 gactgcgcgg ccggcatcga gcagcgccac actttgtggt gtcgtcacga tgactgcacc
     2881 gtctgtctgc actagctgcg caagcgagag ctgggtgtcg cccgtgccag gtggcaggtc
     2941 aataatcagg aagtcgagct ccccccaatt catatcatag agaaactgtt cgactgcctt
     3001 gccgagcata gggccgcgcc agactatcgg ctggttgtcg tcaacgagga atgaaaaact
     3061 catcagcttc aggccgtatt tttcgacggg agtgatgcgg tcatttttca cgtcgatctg
     3121 ttgccggccc gaaaggccga gcattttacc caccgacggg ccatagatgt cggcatccat
     3181 caatcccact tttttaccca tctgctgcag cgttgaggcg aggttaacag atactgtgga
     3241 tttaccaacg ccacccttac cggaagctat ggcaatgacg tgcttcacgc ctggaatgct
     3301 gcgctgtggc tttgctaccg ggccctgctt tgttacttca ggtttggcgt cggaccattt
     3361 gaatttgaaa ccatcgggcg cgatgccttc tttactcgct agtgtgcgca cctgagcttc
     3421 gattgccagc tgccacttct tgtcgcctgc ggccttgagc accagcaccg gtttctcgcc
     3481 gtcttcaatg gcgatgaaaa gtttgtcatc ggcgatcgac ttgtgcgtca gcggattttg
     3541 tacccgcgaa atcagatttt ccagttgttg tttcatttaa cgacccttaa acgtatttgc
     3601 taccgcaatc gcggcaatat taaagaatat accaaaaatt ccgacccaca ggtaattttg
     3661 gattggttgc ccgggtggag ctacaataat gagcgaggcg gccatggccc cgagtgacat
     3721 ggcaaactgc tgcaccgatc cattgaggct catgaacgtg cccctcagcg cggggttgac
     3781 ggtgttgttg agcaatgcca tggccggcac catgcggctc gaaacgaaga caaagaagag
     3841 cacggtaaaa acaatcagca gcgggaaaaa cttcgtccac tcgtaggtaa cgagaataaa
     3901 cggcaggctg gctaccagcg aaagaaaact atagatacga acggggccgt atttgtccgt
     3961 cagggttcct gcaaatggcg acgaaacaat ggtcgccagc ccgcctgtca cgtagatcca
     4021 gaacagctgg tcgtcgctga cgccgttgtt cttctgcatg aagttggcga tgaatggaat
     4081 gatgctgaaa ccagcgacaa agtggaatac catcatcagg tagagacggc gattcttgct
     4141 atccgcgaga acgctgcgga tctgctggat cacctgactg cgtggcccgg cgagatgggc
     4201 atcaaggctg ggcatgcgca gcgcagtgag cgccagcacg gccagcgaac cggcggcgat
     4261 caggtagaat ggcagctgca ggaaaaaccg tcccgcaaac cagatacccg cgggtactcc
     4321 aacaacagcg gccacagaaa acgccatcat gatcacaccc gttgcccggc cgcgcttgtg
     4381 tggttcgatc aggtcgccga tgatcgaaaa ggtcactccg ctcagtaagc caccgaatgc
     4441 accggtgacg acgcgggctg ccaggaagta ccagaaaccg ggcgcaacgg cgcagaacaa
     4501 atttcctgcg atgaaacctg cgctgccaat cagcaacgaa gccttacgag agacgcgatc
     4561 gatgaagaag ctacccgcga tacctgagaa aaaagcggcg aaggtatagc tagagaccag
     4621 ccagccaaat tggttggtgc tgatatgaaa ttcaacctcg agtttgcgcg ccagcggcat
     4681 gatcaccatg aaatcgacga tgtgcatgaa ctggatcact gccagtatgc cgagtaccgt
     4741 ccactgcgtg cggctaaaaa ccggctggtc gga
//
LOCUS       DAOQJF010000263         4768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10594_length_4768_cov_4.106726, whole genome shotgun sequence.
ACCESSION   DAOQJF010000263 DAOQJF010000000
VERSION     DAOQJF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10594_length_4768_cov_4.106726"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLF85_12740"
     CDS             complement(<1..414)
                     /locus_tag="PLF85_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNE20585.1"
                     /translation="MKSLREFFSSLYRPATGNLPRIVILISGRGSNMRALLEKIREGR
                     LRAQCTLVLSDREAKGLEVARAMGFRAELFPRKKEESRESFDRRLAERVKKENPALVV
                     CAGYLRILSEPMLTAFPQRIINIHPSLLPQFPGLEA"
     gene            complement(411..884)
                     /locus_tag="PLF85_12745"
     CDS             complement(411..884)
                     /locus_tag="PLF85_12745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNE20586.1"
                     /translation="MAEKQKPWEKFSVTVETVVRWRDLDAYNHVNNSVYLNYFEEGRI
                     AYYFKLAELAGIPADNPEIFDGFSTVLANTNIEFKGQGRLHETLVIGIRYTAIRKIFL
                     EAEYGIFNKATGQLLARGTSTQVAVDLKNNKPMRVNAAFTAMAQKLEGDRLVVVQ"
     gene            complement(889..1863)
                     /locus_tag="PLF85_12750"
     CDS             complement(889..1863)
                     /locus_tag="PLF85_12750"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE20587.1"
                     /translation="MSTGKGQHALVTGGAGFIGSHLVRELLARGYTVTVWDNLHTGRE
                     KNLAAVRDKIEFRNFDIRGASVADAAALANGKVDVIFNLACPASPPHYQKDPVYTWET
                     SVLGIRNMLLIAEAQGARLLHASTSEIYGDPQVHPQPEQYWGNVNTVGVRSCYDEGKR
                     AAETICADYRRFKNVDARLFRIFNTYGEFMHPDDGRVISNFCVAALKGESVPIYGNGE
                     QTRSFCYASDLVAGILALADLPREKYDGPINLGNPGEFSIRELVGVVEKVLGKKLQTQ
                     NFPLPSDDPKMRRPVIDRAKQVLGWEPKVPLEEGLKKTLAYFAEEIRG"
     gene            1941..2906
                     /gene="pdhA"
                     /locus_tag="PLF85_12755"
     CDS             1941..2906
                     /gene="pdhA"
                     /locus_tag="PLF85_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNE20588.1"
                     /translation="MDTATQRLFRMYEKMLLIRRFEEASAKAYATGKIRGFCHLYIGE
                     EPVAVGAIENLEKQDYVICTYREHGHALARGMSSREVMAELFGKATGCSKGLGGSMHL
                     FSKELNFMGGHGIVGGHIAVAAGHAFASKYRRDRAVTLCFIGEGGTNIGSFYEGLTYA
                     SLWNLPVVYIIENNLYAMGTPIYRAQPTRDLTAKARAFNMPSHRIDALDVELVYNTVR
                     EAVVRARSGQGPSLIEAITYRFRGHSMSDPAKYRPPGELEEMKEKDPLDTTRQRLLQQ
                     GFTDADLQNSEDKVIAEVEDAVQYADASPELGYAEMQRMVYASLS"
     gene            3063..4571
                     /locus_tag="PLF85_12760"
     CDS             3063..4571
                     /locus_tag="PLF85_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNE20589.1"
                     /translation="MYARIFTAARPAFNATLVSVEADVLPGLPNLSIVGLPDHALREG
                     RDRIRAAIVNAGFHFPARHIVINLSPNDIPKEGGLLEAAMAAAILVATGQLPAEPFHD
                     TALLGALSLSGEITVARGLAAAAIFAAGQKKIRRIIVPQIDTGLVTVPEEISLFTLRS
                     IAELPLFCHGGINAAESGRYTPQLPEVDLPIEHIFGLSAAKRALAIAAVGGHHVILAG
                     TPGTGKSLLARAYRHLLPAPEFAEAAEITRVYSLAGLGSGELMAERPFRSPHHTTSTP
                     ALVGGSTLIRPGEISLAHKGVLFLDELPEFRNEALQSLREPLEEKRITIARARGAITY
                     PADFQLIAAANPCRCGNLFIDGGRCSCRKGTGLDQFAKLIGPFLDRIAIELNLNELPS
                     WPLDEEEYTTARLQARIAERRAASRERNGGLLNNSLEAAMLYRRLSHMNPQRLYERYA
                     PQQRLSMRSWLNVLRVAQSIADFEDSEVTELNVHEALSYRFIQRKLASLKAA"
     gene            4579..>4768
                     /locus_tag="PLF85_12765"
     CDS             4579..>4768
                     /locus_tag="PLF85_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20590.1"
                     /translation="MKTAKKQGVETERSRGQAIYGRFAVGQSAAGPAAMHAALASGCG
                     TQSQRLRRARDFFDTLLGP"
BASE COUNT          986 a         1427 c         1379 g          976 t
ORIGIN      
        1 tgcttcgagc cctggaaatt gcggcagcag cgacgggtgg atgttgatga tgcgctgcgg
       61 aaaggccgtc agcatgggtt ctgacaagat gcgcaggtaa ccggcacaca ccacgagcgc
      121 gggattttct ttttttacgc gttcggccag ccgtcggtcg aatgactcgc gcgactcttc
      181 tttcttcctg gggaaaagct ctgcccgaaa gcccatagcg cgtgccactt ctagcccctt
      241 cgcttcgcgg tcgctgagga caagagtgca ctgcgcacgc aacctaccct cgcggatttt
      301 ttcgagcagc gcgcgcatgt tactaccccg gcctgaaatc aatatgacga tgcggggcag
      361 atttcccgtc gctggccggt acaaagacga aaagaactcc ctcaggctct tcattgcaca
      421 acgacgagcc ggtcgccttc aagcttctgc gccatcgcgg tgaaggccgc attgacgcgc
      481 atgggtttgt tgttcttcag atcgacggca acctgtgttg aagtaccgcg cgccagcagc
      541 tggccggttg ccttgttgaa tataccgtat tcggcttcga gaaatatctt gcggatggca
      601 gtatagcgaa tgccgatcac gagggtctcg tgcagcctgc cctgcccctt gaattcaata
      661 ttggtattcg cgaggacagt cgagaagccg tcgaaaatct cggggttatc ggccggtata
      721 cccgcgagtt cggcaagttt gaaatagtac gcaatacggc cttcttcaaa gtagttcagg
      781 tacacgctgt tattcacgtg gttgtaagca tcgaggtcgc gccagcgcac cacggtctcc
      841 actgtaacgg aaaatttctc ccagggtttc tgtttttctg ccatgcgact acccgcggat
      901 ctcttcggcg aaatacgcga gggttttttt gaggccctct tcgagcggga cctttggttc
      961 ccagccgaga acctgctttg cgcggtcgat caccggccgg cgcatcttcg ggtcgtcaga
     1021 aggcagggga aaattctgtg tctgcagctt cttgcccagg accttttcta caacgccgac
     1081 gagttcacgt atcgaaaatt cgccggggtt acccaggttg atcgggccat cgtatttctc
     1141 gcggggtaaa tccgccagcg ccaggatacc cgccacgagg tctgaagcat agcagaacga
     1201 acgcgtctgt tcgccgttgc cgtagatcgg cacactttca cccttgagcg ccgcgacaca
     1261 aaaattcgaa atcacgcggc cgtcgtcggg gtgcataaat tcgccgtagg tgttaaaaat
     1321 acggaatagc cgcgcatcga catttttgaa gcgccggtaa tcggcgcaga tagtttcagc
     1381 tgcgcgcttg ccttcgtcgt aacagctgcg cacgcccacc gtattgacat ttccccagta
     1441 ctgttcgggc tgcgggtgca cctgcggatc gccgtaaatt tcagaagtcg aggcatgcag
     1501 caggcgcgcg ccctgcgcct cggcgatcag cagcatattg cgaataccga ggacagaagt
     1561 ttcccaggta tagaccgggt ctttctggta gtgcggtggc gatgccgggc aggcaagatt
     1621 aaagatgacg tccaccttgc cgttggcgag ggctgcggca tcggccacgc ttgcgccgcg
     1681 aatatcaaag ttgcggaatt caattttgtc gcgcacggcg gcgagattct tctcacggcc
     1741 ggtgtgcagg ttatcccaga cggtgaccgt ataaccgcgc gccagcagct cgcgcacgag
     1801 gtgagaaccg atgaagcccg cgccgcctgt taccagtgca tgctgccctt tgcccgttga
     1861 catatggtat tttctgaaag cgagacaggc taacaataat ttgctattga cgcatcagct
     1921 tcggccccca tgctctgccc atggatactg ccacgcagcg cctgttccgt atgtacgaaa
     1981 agatgctgct gatccgccgc tttgaagagg cgtcggcgaa ggcatacgcc accggcaaaa
     2041 tacgcggctt ctgccacctc tacatcggcg aagagccggt ggctgtcggc gccatcgaaa
     2101 acctcgaaaa gcaggactac gtgatctgca cgtaccgcga gcacgggcat gcgctcgcgc
     2161 gcggcatgag ttcgcgcgag gtcatggcgg agctttttgg caaggcaacc ggctgcagca
     2221 aggggctggg tggctcgatg cacctgtttt cgaaggagct caacttcatg ggcggccacg
     2281 gcatcgtcgg tgggcacatc gccgtcgcgg cgggccatgc gttcgcctcg aaataccggc
     2341 gcgaccgcgc ggtgacactc tgctttatcg gcgaaggcgg aacgaatatc ggcagtttct
     2401 acgagggatt gacctatgcc tcactgtgga acctgcccgt ggtctacatt attgaaaaca
     2461 acctctacgc gatgggtacc cccatctacc gcgcgcagcc gacgcgcgac ctgacggcga
     2521 aagcgagagc attcaacatg ccctcgcacc gcatcgatgc actcgacgtc gagctggtct
     2581 acaatacagt ccgcgaggca gtcgtgcgcg ctcgctctgg ccaggggccg agccttatcg
     2641 aggcgatcac ctaccgcttc cggggacaca gcatgtctga cccggcaaag taccgcccac
     2701 ccggcgagct cgaagagatg aaagagaaag acccgctcga tacgacacgg cagcgcctgc
     2761 tgcaacaagg ctttaccgat gctgatttac agaactccga agacaaggtt attgccgaag
     2821 tcgaagacgc agtgcagtat gctgatgctt cgcccgaact gggttatgca gagatgcagc
     2881 gcatggttta cgcgagtctc agctgacatc tacccgatat gaattttttt caaaatccgt
     2941 gagaaaaaac cggcgtttgc tggtatttat tatgtaggga cggcatgaag ccaaggcgtt
     3001 tgcccgcaaa cgcccaggct gtccgccgtc cccaaaatac ctcgtgtacc ataggagcga
     3061 ttatgtacgc acgcatcttt accgccgcac ggccggcatt taacgccaca ctggtttcgg
     3121 tcgaggccga cgttctgccg ggtctgccca acctgagtat tgtcggcctg cccgaccacg
     3181 cgctgcgcga aggccgcgac cgcatcaggg cggcgattgt gaacgcgggg tttcattttc
     3241 ccgcgcggca catcgtgatc aacctctcgc ccaacgatat accaaaagaa ggtggcctgc
     3301 ttgaagcagc catggcggca gcaattcttg tggccacggg gcagttgccc gccgaaccat
     3361 tccacgacac ggcactcctg ggtgccctgt cgttatcggg cgagatcacc gtggcgcgcg
     3421 ggcttgccgc cgccgcgatc tttgccgcag gacagaaaaa aatccgccgc atcattgtgc
     3481 cgcagattga tacggggctc gtcaccgtgc ccgaagaaat ttcgctcttc acgctgcgca
     3541 gtatcgccga gctgccgctg ttctgccacg gcggcatcaa tgccgccgag agcggacgct
     3601 atacaccgca gctgcccgaa gttgatctgc cgatagagca tatttttggc ctcagcgcgg
     3661 caaaacgtgc gctcgcaatc gccgccgtgg gagggcacca cgtgattctg gccggcacac
     3721 ccggcaccgg caaaagcctg cttgcgcgcg cttaccgcca cctgctgccg gcccccgaat
     3781 ttgctgaagc tgctgaaatc acgcgcgtct actcgctcgc aggcctgggc agcggcgaac
     3841 tcatggctga acgtcctttc cgctctccgc accatacaac ctcgactcct gcactggtag
     3901 gcggcagtac gctcattcgc ccgggtgaaa tcagccttgc gcacaagggt gtgctctttc
     3961 tcgacgaact acccgaattc cgtaacgaag cgctgcagag cctgcgagag ccgctggaag
     4021 aaaagcgcat cacgattgcg cgcgcgcgtg gtgcgatcac ctaccctgcg gatttccagc
     4081 tcattgccgc ggcaaatccc tgccgctgcg gcaacctgtt catcgacggc ggccggtgca
     4141 gctgccgcaa aggcacggga ctcgaccaat ttgccaaatt gattgggccg ttcttagacc
     4201 gtatcgccat tgaactcaat ctgaacgaat taccttcgtg gccgctggac gaagaagaat
     4261 acactacggc aagactacag gcgcgtattg ctgagcggcg cgctgcgtcg cgcgagcgta
     4321 acggggggtt gctgaataac agcctcgagg ccgcgatgtt ataccgccgc ctgtcgcata
     4381 tgaacccgca gcggctctat gagcggtacg cgccgcagca gcggctgtcg atgcgctcgt
     4441 ggctgaacgt gctgcgcgtc gcgcagtcaa tcgccgattt tgaagattca gaagtgacgg
     4501 aactcaatgt ccatgaggcg ctgagctacc gtttcataca acggaagctc gcgagtctca
     4561 aggctgccta acaggctgtt gaaaacagcc aaaaagcagg gtgttgaaac cgagcgcagt
     4621 cgcggacagg ccatttatgg ccgttttgcc gtcggccaaa gcgccgccgg ccccgcggct
     4681 atgcacgccg ctttggctag cggttgcggc acgcaatcgc agcgtctgcg acgagcacgg
     4741 gactttttcg acaccctgct aggcccgg
//
LOCUS       DAOQJF010000264         4762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10616_length_4762_cov_4.631825, whole genome shotgun sequence.
ACCESSION   DAOQJF010000264 DAOQJF010000000
VERSION     DAOQJF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10616_length_4762_cov_4.631825"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="xerD"
                     /locus_tag="PLF85_12770"
     CDS             complement(<1..859)
                     /gene="xerD"
                     /locus_tag="PLF85_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNE20591.1"
                     /translation="MSKTAAKRNFVSNFKEYLSIERGLSENSIFSYVSDVQKLEKFLT
                     KEKKQLQSSSEKDIANFLREESRKKLSSRTRARVVASLRQFYGYLENRKLIEVNPMQA
                     IEAPKIEKALPDFLTQEEIRKLFEVFREDNVLELRDKTMFEFLYSSGLRISEACSLLM
                     TDVDRENQILTIKGKGGRERLVPYGEVAAKLLVQYLDRARAEILNQYASDFVFVSKKG
                     GALSRKSAWRLLKRYMKRAGIGRNITPHTLRHSFATHLLQNKADLRAVQELLGHMDIA
                     TTQIYTHLAS"
     gene            complement(1070..2386)
                     /locus_tag="PLF85_12775"
     CDS             complement(1070..2386)
                     /locus_tag="PLF85_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HNE20592.1"
                     /translation="MRRSFIVGLLFLCLIAPAGAKGKRPRLIIWSIDGFAAGYLKQPR
                     FAENENWQYLLRRARVYGDVETTVPAVTYPAHTSMITGAHPARHGIQSNHPVDPFNLA
                     KGGWTWYTADVHARQLWDLAREQGKSVANMQWPVTMTQTSRIRWHIPQFDRARGPEEA
                     KMMRVISTPGLHREIEKETGVSLTEYSTDRERLNAALYVWEAKKPDLMLLYHPGLDAT
                     EHAAGPYSPAAYAALDDLGKMAEKLVRKVRRQGHENTAVLIVSDHGFMTFRGRCYPNS
                     LLLQQGFINAEQKTWSFYFDTAGGVARLVRNTGVTQQFPAARFAALVSKQCPGIEYVD
                     ADHVDYEYLRTHYSKNAEGFLVSRSNTVMSPSWGAQIFDEAATGHTHGFLPQREDMKT
                     VAIAFTGNTRKTVPIRHVTDTYRFACRWLRLECPAQKNVHRAEPAE"
     gene            complement(2390..2875)
                     /locus_tag="PLF85_12780"
     CDS             complement(2390..2875)
                     /locus_tag="PLF85_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20593.1"
                     /translation="MRFIAFLVTVLLPLPLLADSLECLAEGTVEQIAEQLPPGSVLYR
                     DCYHCQQPAYEVIKIEKTELRPCHLPDSQSDRALYISGTVQRRFQMEKCAEPKNEEQV
                     KLKLSGELLVLNYAWLYEKKTGEATNIADMFGENSHHLCKRFTDRPLKPKTRPGKKSG
                     G"
     gene            complement(2944..3249)
                     /locus_tag="PLF85_12785"
     CDS             complement(2944..3249)
                     /locus_tag="PLF85_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20594.1"
                     /translation="MGFWSSIKDAFSGKDDATRIQELAAELSRYADRYENAGDAENAA
                     LARDYAERVASARSLKEAKALYSEFKAVTSKYHDEGYHERQHHAIDDGREDTWSDNS"
     gene            complement(3249..4208)
                     /locus_tag="PLF85_12790"
     CDS             complement(3249..4208)
                     /locus_tag="PLF85_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20595.1"
                     /translation="MKTAVITCFFNPQHYRSRLENFAIFARAMRRRRIPLFAVEAVFA
                     GEEGTVGKFADTLTVSCEAVLWQKESLLNHLLQQLDKRYEAVVWCDADVLFENTTWFN
                     ALKRQLKKYAVVQPFAEALRLPRGARRATKSAERFASFGAVYEQKPQLLLKGDFAAHG
                     HTGFAWAARREVLSEHGLYDAMIAGSGDHVMAHAFAGDFSSRCIRRIMADNEPHIDHF
                     ARWATRVYPQVRGKVGFVPGRLLHLWHGDTENRRYVDRNRELAAFGFDPEKHLERDAR
                     GLWRWRKSKPGLRGSLERLTGGSKHRQMQDWAIRYFASRREDG"
     gene            complement(4319..>4762)
                     /locus_tag="PLF85_12795"
     CDS             complement(4319..>4762)
                     /locus_tag="PLF85_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HNE20596.1"
                     /translation="KSSAALLGITLFGSAFVAYYSGNLEALDTMDFWAGTFFIFVLAI
                     FQVVVAGWLFGVEKVLAEAAEGSRMKVPRLLPFLIKYVSPAFLIAIFAMWCWQKLPEQ
                     WLKIQSNTVTQITVLFLLAALLLFLGMTYFAVARWQHREERRHHP"
BASE COUNT         1018 a         1337 c         1341 g         1066 t
ORIGIN      
        1 cagaagcgag gtgggtatag atctgcgtcg tggcgatatc catgtgtccc aggagttcct
       61 gtactgcgcg caggtctgcc ttgttctgca gcaggtgtgt cgcgaatgag tggcgcagcg
      121 tgtgcggagt gatgttgcgg ccgataccgg cacgcttcat gtagcgcttg agcagccgcc
      181 aggcgctctt gcgtgacagt gctccaccct ttttagagac aaagacaaaa tcagaggcat
      241 actggttgag aatctccgcg cgcgcgcggt cgaggtactg cacgagcagc ttggccgcta
      301 cttcaccata gggtaccagg cgttcacgac cgcctttgcc tttaatcgtg agaatctggt
      361 tctcgcggtc aacgtcagtc attagcagcg agcatgcttc agaaatgcgc aggccggaag
      421 aatacagaaa ttcgaacatg gtcttgtcgc gcagttcgag tacgttatct tcacggaaaa
      481 cttcgaagag cttacgaatc tcttcctgcg tcaggaagtc tggcagcgct ttttcgatct
      541 tcggcgcttc aattgcctgc atcgggttca cttcgatcag cttgcggttc tcgaggtaac
      601 cgtagaactg gcgcagtgag gccacaacgc gggcgcgtgt acggctcgag agcttcttgc
      661 gcgattcttc gcgcaggaag ttggcgatgt ctttttctga acttgattgc agctgcttct
      721 tttctttggt caggaatttc tcgagtttct gtacgtctga gacataggag aaaatgctgt
      781 tttcggacag gccccgctca atgctgaggt actccttaaa attactgaca aagttacgtt
      841 tagcggctgt tttgctcatc gggtgtcctt agtttagggt catttaccgc agcagaggcc
      901 gggtattacc cgcaaaccgc cctgttgtat tatgacgcat gttatgtatt gattttcgac
      961 gcctgcgatt tttattttga taaaaaaatt atgtctcgct cgcagtaggg ggctgcgcgg
     1021 cagctcgcgg acgccggcaa aaaaggcgta aatgcgcggt aattcgggtt tattctgccg
     1081 gttctgcgcg gtgaacgttc ttctgcgcag gacactcaag ccgcagccag cggcaggcaa
     1141 agcggtacgt gtcggtcacg tggcgaatcg gcacggtttt gcgcgtattg cccgtgaaag
     1201 caatggccac tgtcttcatg tcttcacgct gcggcaaaaa gccgtgcgta tgcccggtag
     1261 ctgcttcgtc aaaaatctgc gctccccagc tggggctcat gacggtatta gagcgcgaga
     1321 cgagaaaacc ttcggcgttc ttcgagtagt gcgtgcgcag gtattcgtag tccacatggt
     1381 cagcgtcgac atattcaata cccggacact gcttgctgac gagcgcagca aaacgcgcgg
     1441 cgggaaactg ctgcgtgaca ccggtattgc gcacgaggcg cgcgacgccg ccagccgtat
     1501 caaagtagaa gctccacgtc ttctgctcgg cgttgataaa cccctgctgc aacagcagcg
     1561 agttcgggta gcacctgcct cgaaaggtca tgaacccatg gtctgagaca atcagcactg
     1621 cagtgttctc gtgcccctgg cggcgcacct tacgcaccag cttctctgcc atcttgccga
     1681 ggtcgtcgag cgccgcataa gctgccggcg aataaggccc agccgcgtgt tcggtcgcat
     1741 cgagccccgg atggtagagc agcatgagat cgggcttttt tgcttcccag acataaagag
     1801 ctgcgttgag ccgctcgcgg tcggtgctgt attctgtcag cgagacgcct gtttctttct
     1861 caatctcgcg gtgcagcccg ggcgtgctga tgacgcgcat catcttggcc tcttcgggac
     1921 cgcgtgcgcg gtcgaactgc ggtatgtgcc agcgtatgcg cgatgtctgc gtcatcgtga
     1981 ccggccactg catgttggcg acggattttc cctgttcacg agcgagatcc cacagttggc
     2041 gggcatgcac gtcggccgtg taccatgtcc agccgccttt cgcgaggttg aacggatcga
     2101 ccggatggtt cgactgtata ccgtggcgcg caggatgcgc ccccgtgatc atcgaggtgt
     2161 gtgctgggta tgtcaccgca ggcacggtcg tctcgacatc gccgtagacc ctggcgcgac
     2221 gaagcaggta ttgccagttt tcattttctg caaaacgcgg ctgcttcagg taaccggcgg
     2281 caaaaccgtc gatcgaccag atgatcagcc gcgggcgttt acctttggcc ccggcgggag
     2341 caataaggca aagaaacaat aagccgacga taaagctgcg ccgcatacct tagccgccgg
     2401 attttttgcc aggcctggtt ttgggtttta gcgggcgatc ggtgaaccgc ttgcagaggt
     2461 ggtgcgaatt ttccccgaac atgtctgcaa tgttggtcgc ctcaccagtt tttttctcgt
     2521 agagccaggc atagttgagc accagcagtt cgccagagag cttcagcttc acctgctctt
     2581 cgtttttcgg ctcggcgcac ttttccatct ggaagcgccg ttgtaccgtc ccggaaatat
     2641 acagggcacg gtcgctctgc gaatcaggca ggtggcaggg acgcaactcg gtcttttcta
     2701 ttttgatcac ttcgtacgcg ggctgctggc agtggtagca gtcacggtag agcacactac
     2761 ccggtggcaa ctgctctgcg atctgttcta cagtgccttc ggcaaggcat tcgagcgaat
     2821 ctgcaaggag tggcagcggc agcaggactg taaccaggaa tgcaataaaa cgcatgcacc
     2881 aaggctgatt atttgcctgt gcgataaagc aacttgcggc tgcgggcggc tcaggcagga
     2941 atcttacgaa ttatctgacc aggtgtcttc gcggccatcg tcaatggcgt ggtgttgacg
     3001 ctcgtggtag ccttcatcgt ggtatttgct ggtaacagcc ttgaactcgc tatagagcgc
     3061 tttcgcttct tttaaggaac gcgcagaggc tacgcgctca gcgtagtcgc gggcaagtgc
     3121 cgcattttct gcatcacccg cattttcgta gcgatcggca taacgtgaaa gctccgccgc
     3181 gagctcctgg atacgggttg catcgtcttt gcccgaaaaa gcgtctttaa ttgaactcca
     3241 aaaacccatt agccgtcctc ccggcgcgat gcaaaatagc gtattgccca gtcctgcatt
     3301 tgcctgtgtt tgctgccgcc agtaagcctc tctaagctac cgcgtaaacc cggtttactt
     3361 ttacgccagc gccacagacc gcgtgcatcg cgctcgaggt gcttttccgg gtcgaaacca
     3421 aaagccgcca gctcgcggtt gcggtcgacg taacggcggt tctcggtatc gccatgccag
     3481 aggtgcagca ggcgacctgg cacaaaacct actttgccgc gcacttgcgg ataaacccga
     3541 gtcgcccagc gcgcgaaatg atcaatgtgt ggctcgttgt cggccatgat acgccttatg
     3601 caacgcgatg agaagtcgcc ggcaaaggca tgcgccatca catgatcgcc cgaaccggca
     3661 atcatcgcat cgtacaggcc atgttcagag agaacttcac gccgcgccgc ccacgcaaaa
     3721 cccgtgtgcc cgtgcgcggc aaagtcaccc ttgagcagga gctgcggctt ctgctcatac
     3781 accgcgccaa aactcgcgaa acgttcggcg cttttcgtcg cccggcgtgc accgcgcggc
     3841 aggcgcagcg cttcagcaaa tggttgcacc accgcgtact tcttcagctg gcgcttgagg
     3901 gcattaaacc aggtagtatt ttcaaacagc acatcggcgt cacaccagac gacagcctcg
     3961 tagcgtttgt ccagctgctg aagcaagtgg ttgagtaatg actctttctg ccacaaaact
     4021 gcttcgcagc ttaccgtcag ggtatcggcg aatttgccaa cagtgccctc ttcgcccgcg
     4081 aagactgcct cgaccgcaaa cagcggtata cggcggcggc gcatcgcgcg cgcaaaaatg
     4141 gcaaaattct ccagccgcga gcggtaatgc tgcgggttga agaaacacgt gatgacagcc
     4201 gtcttcacga taactgcacg ctattcagga tctttgcgca gaacataata gaggctgacc
     4261 gcaaagaata cggtcacaaa cgaaatcgag agaatcatta ccagccagcc gccggtagtc
     4321 atgggtggtg cctccgttct tcgcgatgct gccagcgggc aacggcgaaa taagtcatgc
     4381 cgagaaagag taacaacgcc gccagcagga agagtacggt gatctgcgtc accgtattcg
     4441 actgtatctt cagccactgc tcgggtaatt tctgccagca ccacatggca aagatcgcaa
     4501 tcaaaaaggc cgggctcacg tacttgatca ggaaaggcag caaccgcggc actttcatgc
     4561 gcgaaccttc agcggcttcg gccagtacct tttcaacgcc gaacaaccag ccggccacaa
     4621 cgacctggaa gatcgccagc acaaagataa agaaagttcc cgcccaaaag tccatcgtat
     4681 cgagcgcctc aaggttgccc gagtagtacg caacaaatgc cgaaccgaaa agcgtgatac
     4741 ccagcagcgc agcagagctt tt
//
LOCUS       DAOQJF010000265         4761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10618_length_4761_cov_4.234169, whole genome shotgun sequence.
ACCESSION   DAOQJF010000265 DAOQJF010000000
VERSION     DAOQJF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10618_length_4761_cov_4.234169"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLF85_12800"
     CDS             <1..1025
                     /locus_tag="PLF85_12800"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNE20597.1"
                     /translation="WSALDEITTVRLTPYGARLVSLSVPDRNGNLVDVVTGYDSEEDY
                     RKGARFFGANVGPFANRIANARFAIDGTEYRFTPNGNGHLLHSGEQGLDSVLWNCARG
                     ERSRTTAATNGIAFTYHSPDGEFDFPGPVDYSIHYKLLPGPTLRIEYTAVPQQATHLN
                     IAHHSYFNLAGHDAGSIDDHLVQINAAHYLEVDADSIPTGKLLPVAGTPLDLRDPTVI
                     RERLKADFLPLRTCGGFDQCFVVDTSRPPTAGLYFAARVVAPQTGIAMEIHSSYPALQ
                     FYTANHDLPPVPGKGGAVYRRHGSFCLEPQFFPNSPNIAHFPSTLVRPGETYRQVVEF
                     RLARSA"
     gene            1094..2737
                     /locus_tag="PLF85_12805"
     CDS             1094..2737
                     /locus_tag="PLF85_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HNE20598.1"
                     /translation="MGLLKPTPPPYDPVAWQNKPFPEQVRLACQAWAVQGYGTPLGAY
                     GLYVFKVIFYIYAWAFFCSFTPGMGGVGEWQNWIFEPIAFQKAILWSMLFEVLGLGCG
                     SGPLTGRYFPPIGGFLYFLRPGTTKLALYPRLPLLGGATRSILDVGLYAALLVSLGWV
                     LTRATITFEDVLPVAVMLPLLGAMDRTLFLAARGEHYWVTAVCFLFAPAWIGGAKMVQ
                     LTLWFFAGFSKLNRHFPYVVGVMNSNSPFTRFEFMRKFMFKDYPNDLNPSRFARLAGH
                     WGTAVELTIPLFFLFSPVGSFGYYAGFALMLGLHIYITSNVPMGVPIEWNFMVVYAAF
                     FFFGAHHAVPITSSPAELLVFVGFCSIALPVIGNIYPHALSFLISMRYYAGNWPVSVW
                     LFKKGSHEKLKKLTTSARWVPDQLRLFYSEADTAAILSKVMAFRLMHLHGRAFSALIP
                     RAVPRVEDYTYAEGEVIAGQIIGYNFGDGHLHNETLARLVQQQCNFDSGEVRCVFIEG
                     EPLFRRTLSYRLYDLKDGQIKAGEMLVDELVERQPWEIP"
     gene            2734..4158
                     /locus_tag="PLF85_12810"
     CDS             2734..4158
                     /locus_tag="PLF85_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE20599.1"
                     /translation="MTDAVVVGSGPNGLAAAIRLAQAGRSVVVLEAHDEIGGGTRTSE
                     LTLKGYKHDVCAAAHPMGIVSPFLRSLPLEKYGLEWIFPEASVAHPLADGRAVLLTQS
                     LEETADRLGVDGKAYHKLLKPFVCCSDALIEEALTVSLTRIPRHPLLMARFGLKALRS
                     AEALAARFKTEEARALVAGCAAHSILPLEQSLTAAVALVFLITAHMHNWPVVKGGSHA
                     ITQAMAKYFKSLGGTIRTGQRVASPGDLPEARAYLFDTSPDLLANHFAGVLPAGYVSR
                     LKKFRYGPGVFKVDYALKGPIPWQNADCLKASTVHLGGSFEEIAAAEAAVWKGEHPAQ
                     PYVLLTQQSQFDKTRAPKGRHTLWAYAHVPRNSTFDMSEIIENQVERFAPGFKKLVLK
                     RHSMNCRDFHSYNANYVGGAITGGVNDLCQFLTRPVARLNPYSTPNPKIFLCSASTPP
                     GGGVHGMCGFNAANTVLNTLTRRA"
     gene            complement(4163..4633)
                     /locus_tag="PLF85_12815"
     CDS             complement(4163..4633)
                     /locus_tag="PLF85_12815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE20600.1"
                     /translation="MHNQDLVFDIIPKDLFDIRRDGTLVESIQHLIEQCHPGTTKMKL
                     ETLSGEESVASIPFAMSNRALHGLLHGGAYFTVGDTITAMMCLFFVEEPNERMVTVNA
                     SIRYLRPVNRETIKARALLKRREGKNLYFVCDFFLPDGKRAAQAKYHYVLAQIR"
BASE COUNT          920 a         1449 c         1368 g         1024 t
ORIGIN      
        1 attggtctgc actcgatgaa ataaccaccg tgcggctgac cccgtacggc gcgcgcctcg
       61 tctcgctctc ggtacccgac cgcaacggta acctcgtcga cgttgtgaca ggttacgaca
      121 gcgaagaaga ctaccgcaag ggtgcgcgct tcttcggggc gaatgtcggc ccgtttgcca
      181 accgcatcgc caacgcacgc tttgcgatcg acggcaccga ataccgtttc acacccaacg
      241 gcaacggcca cctgctgcac agcggcgaac agggcctcga ctcagtgcta tggaattgcg
      301 ctcgtggtga gcgcagtcga accacagccg cgaccaacgg aattgccttc acctaccaca
      361 gccccgacgg cgagtttgat tttcccggcc cggtcgacta tagcattcac tacaaacttc
      421 tgcctggccc gacgctgcgc atcgaataca ccgccgtgcc gcaacaggcg acgcacctca
      481 acatcgcgca ccattcgtac ttcaatctcg cgggccacga tgccggcagc atcgacgacc
      541 atctcgtgca gatcaacgcg gcacattacc tcgaagtcga tgccgattcg ataccgacgg
      601 gaaaactttt gcctgtcgcg ggcactccgc ttgacttacg agatcccact gtgattcgtg
      661 aaaggctcaa ggcggatttt ctgccgttac gaacctgcgg cggttttgat caatgcttcg
      721 tagtcgatac gtcgcggccg ccgactgcgg gcctctattt cgctgcgcgc gtggtggcgc
      781 cgcagaccgg catagctatg gaaatccata gcagctaccc ggcgctgcag ttctacaccg
      841 cgaaccacga cctgccgcct gtgccaggca aagggggcgc ggtctatcga aggcatggtt
      901 cgttctgcct cgagccgcag ttttttccca actcgccgaa catcgcgcac tttccttcga
      961 cgctggtgcg ccccggtgaa acctaccggc aggtggtcga gttccgcttg gcgcgctcgg
     1021 cgtaaccggc cgccggcccc ggcagcggtg aacgccattc ttgacggcgc gaaccaccgg
     1081 tggtcgatgg gtcatgggcc tactcaaacc aacaccgccg ccgtacgacc ccgttgcatg
     1141 gcagaataaa cccttccccg aacaggtgcg ccttgcgtgc caggcctggg cggtgcaggg
     1201 ttatggcaca ccactgggcg cgtatgggct ctatgtcttc aaggtcatct tctatatcta
     1261 tgcctgggca ttcttctgtt cgttcacgcc gggtatgggc ggcgtgggcg aatggcaaaa
     1321 ctggatcttc gagccgattg cattccagaa agccattctc tggagcatgc tctttgaagt
     1381 gctcgggctc ggctgtggca gcgggccttt gaccggaaga tatttccctc ccatcggcgg
     1441 atttctctat tttctgcgac ccggtaccac caagctggcg ctctaccccc gcctgccgct
     1501 gctcggcggt gcgacgcgca gtatactcga cgttggcctg tatgcggcgc tgctcgtaag
     1561 cctgggctgg gtactgacgc gcgcgaccat cacttttgaa gatgtgctgc ctgtcgcagt
     1621 catgctgcct ctactcggtg ccatggaccg cacgctgttt ctcgcggcgc gcggcgaaca
     1681 ctactgggta acggccgtct gcttcctctt cgcccccgca tggatcggcg gtgcgaaaat
     1741 ggtgcagctg acactctggt tctttgcggg cttttcgaaa ctcaaccgcc actttccgta
     1801 cgttgtcgga gtcatgaatt cgaacagtcc gtttacgcgc tttgagttca tgcgcaaatt
     1861 catgttcaaa gattacccga acgatctgaa tccttcgcgg ttcgctcgcc tcgccgggca
     1921 ttggggtact gcggtcgagc tgaccatacc gctgttcttt cttttctcgc cggtcggcag
     1981 ctttggctac tatgctggct ttgcgctgat gctgggcctg cacatctaca tcacgtcaaa
     2041 cgtcccgatg ggagttccga ttgaatggaa cttcatggtc gtctatgctg catttttctt
     2101 ttttggtgct caccatgcag taccgattac ctcgtcaccc gcagaactac ttgtttttgt
     2161 cggcttttgt tcgatagcgc taccggttat cggcaacatt tacccgcatg cgctttcgtt
     2221 tctgatttcg atgcgctact acgccggcaa ctggccggtt tcggtgtggc tgttcaaaaa
     2281 gggctcacat gagaagctca agaaactcac gacgagcgct cggtgggtgc cagaccagct
     2341 gcggctcttc tacagcgaag ccgacactgc cgcaattctg agcaaggtga tggcgtttcg
     2401 gctcatgcac ctgcacgggc gcgcattctc ggcactgata ccccgcgcgg tgccgcgggt
     2461 cgaagactac acgtacgccg aaggcgaggt gattgcgggg caaattatcg gctacaactt
     2521 cggcgacggg cacctgcaca atgaaacgct cgcgcgcctc gtgcagcagc agtgtaattt
     2581 tgattcaggc gaggtgcgct gcgttttcat agagggcgag ccacttttca ggcgcacact
     2641 cagctaccgg ctgtacgacc ttaaagacgg ccagatcaaa gccggtgaaa tgctggtcga
     2701 cgaacttgtg gaacgccaac catgggaaat tccgtgaccg atgcagttgt agtcggttcc
     2761 ggccccaatg ggctggcggc ggctatccgc ctcgcgcagg cgggacgttc ggtcgtcgtg
     2821 ctcgaagcgc acgacgaaat cggcggcggc actcgcacga gtgaactgac actcaaaggt
     2881 tacaaacacg atgtctgtgc cgcagcgcac cccatgggca ttgtttcgcc attcttgcgc
     2941 tcactgccgc tcgaaaagta tggtctcgaa tggatctttc ccgaagcttc ggttgcacac
     3001 ccgcttgcgg atggacgcgc ggtattgctg acgcagtcac tcgaagagac tgcagaccgc
     3061 ctcggtgtcg acggcaaagc ctaccacaag ctgctcaaac ctttcgtctg ctgcagtgac
     3121 gcactgatcg aagaagcgct caccgtctcg ctgacccgaa ttccgcggca cccgctgctg
     3181 atggcgcgct tcggccttaa ggccctgcgc agcgccgaag cgctggcggc acgcttcaaa
     3241 acagaagagg cgcgcgcact ggtcgccggc tgcgccgcgc attcgatact gccgctcgaa
     3301 cagtctttga ccgcggcggt tgcactggta tttcttatca cagcgcacat gcacaattgg
     3361 ccggtcgtca agggcggatc acacgcgatc acgcaggcga tggcaaagta ctttaagtca
     3421 ctcggtggca cgatcagaac aggccagcgc gtggcatcgc cgggtgacct gcccgaagcg
     3481 cgtgcctacc ttttcgatac aagccccgac ctgttggcga atcacttcgc cggcgtgctg
     3541 ccggcaggct acgtctcgcg gcttaaaaag tttcgttacg gccccggcgt gttcaaggtc
     3601 gactatgccc tcaagggacc tattccctgg cagaacgccg actgcctgaa ggcgtcgacc
     3661 gtgcacctcg gcggcagctt cgaagaaatc gccgccgcag aagccgccgt gtggaaaggg
     3721 gaacatccgg cgcagccgta tgttttgctc acccagcaga gccagttcga caaaacgcgc
     3781 gcaccgaagg gcaggcacac gctgtgggcc tacgcacacg taccgcgcaa ctcgacgttc
     3841 gacatgagcg aaattatcga gaaccaggta gaacgttttg cgccggggtt caaaaagctg
     3901 gttcttaagc ggcacagcat gaactgccgc gactttcaca gttacaacgc gaactacgtc
     3961 ggcggagcga tcacgggtgg ggtcaatgac ctctgccagt ttctgacgcg gccggtggcg
     4021 cgcttgaacc cgtattcaac gcccaacccg aaaatctttc tttgttcggc ttcaacgccg
     4081 ccgggcggcg gtgtgcacgg catgtgcggc ttcaatgcgg cgaataccgt cttgaacacg
     4141 ctcacacggc gcgcatgagc cgttaccgaa tctgtgccag cacgtagtgg tacttcgcct
     4201 gcgcggcgcg tttgccgtcg ggtaagaaga agtcgcagac gaagtacagg ttcttgccct
     4261 cgcggcgctt cagtagggcg cgcgccttga tggtttcacg gttcaccgga cgcaagtagc
     4321 gaatcgacgc attcaccgta accattcgct cattgggttc ttcgacgaag aataaacaca
     4381 tcattgcagt gatggtatcg ccgacggtga agtatgcgcc gccgtgcagc aggccatgca
     4441 gcgcgcggtt cgacatggca aacggtatcg atgcgaccga ctcttcgccc gaaagcgttt
     4501 cgagtttcat cttcgttgtg ccggggtggc actgctcgat gaggtgctga atcgactcga
     4561 cgagggtgcc gtcgcggcgt atgtcgaaca ggtctttggg aatgatgtcg aataccaggt
     4621 cttgattgtg catatacacg ttactccgtg agtcacgccg ctttttcccg agtcggcggc
     4681 aagcggttag cgattgcggt tgacgtaggc cataatccgt tgcgaacatc atcgggcagt
     4741 gcgcgagcga ttattcatat t
//
LOCUS       DAOQJF010000266         4739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_10684_length_4739_cov_5.128736, whole genome shotgun sequence.
ACCESSION   DAOQJF010000266 DAOQJF010000000
VERSION     DAOQJF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10684_length_4739_cov_5.128736"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /gene="lpxK"
                     /locus_tag="PLF85_12820"
     CDS             complement(<1..424)
                     /gene="lpxK"
                     /locus_tag="PLF85_12820"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNE20601.1"
                     /translation="MTAQMNPLEKLYSFGNRLHRRMTRSIRIPGAKILSVGNITTGGT
                     GKTPATIYFSRLLRSEFPNQAVLSRGYGGKLSKETNLLSDGVRLLMSSDDSGDEPYLM
                     AVNLPGVRVAVGRNRLRNAQRLVEEHGVDLFLLDDGFQH"
     gene            473..1168
                     /locus_tag="PLF85_12825"
     CDS             473..1168
                     /locus_tag="PLF85_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20602.1"
                     /translation="MAFCLLLFASPTDQFWMGQQPDDTTAFRLLTDFHLKTIAPDRGY
                     LHQVSARMTPVHPATDKKGNVKEAFEKDQDLVKRSAAAKSAAASGTPASAEYRWHVHS
                     PGKDVLYLKPAREIYLPDRNRMFAVYLRGAGHAHQVYAIFRGPNRIQQEIFVCSLDYQ
                     GWKRFEVVIPPYLQLRNPLKQNRYELYFTALKVQSHFRDQPGTSVFNLAEMFIMADTS
                     DKKIPGADMPQEF"
     gene            complement(1203..1817)
                     /locus_tag="PLF85_12830"
     CDS             complement(1203..1817)
                     /locus_tag="PLF85_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20603.1"
                     /translation="MYKAVTRQVVLNDLLRDHVVVHYDRLVSLRLLCTNRSLHLRGVI
                     REGHERVIGLKTGQIYFDFQLKKEEVTAHTVKMRLTKSRFFSSRKPDMIRLSTRFSSK
                     LEDIILYHLTRGDSPFFMVNKKKRFFGFDLNFILMQIPPEVDILGKIKIHNVAFEPRR
                     IVWYLDSNLVLQSLVHIFKPDYIEIEQLDLGADEQKLLTDINLD"
     gene            complement(1827..2447)
                     /gene="upp"
                     /locus_tag="PLF85_12835"
     CDS             complement(1827..2447)
                     /gene="upp"
                     /locus_tag="PLF85_12835"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNE20604.1"
                     /translation="MKAKNYANHALLSAKISVLRDKKTPSPLFARTLHEISLILCALV
                     ASEMETIPRTVATPLVKTRGRVVKSPVVLIPILRAGLGMLEGFRTLLPDAATGHVGVE
                     RDHETLKPKFYYYKTPSLKNADVWVLDPMLATGGSLDFALTKLKQGKPRTLRAISVIA
                     APEGIQLVEKAHPDVKLYTAVVDERLNNKGYIMPGLGDAGDRYFNT"
     gene            complement(2444..3415)
                     /locus_tag="PLF85_12840"
     CDS             complement(2444..3415)
                     /locus_tag="PLF85_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE20605.1"
                     /translation="MIALNGVSKFFAVNRGFLSRDRQTVRALQNVSLEIREKEIVGLV
                     GESGSGKSTLARVIMGLYRPDEGHFTFDGYDSRSMKRDDWQNLRRDIGIVFQDPASSM
                     NPWMTVEQIVAEPMVIRKKKLDLTRAARRDRVAEILAATGMPAETMGRKIHEFSGGQR
                     QRIAIARALVLKPRYLILDEPISALDVSIQAQILNLLKDLHAQYGFAALFITHDLSKV
                     RWFSDRVAVMYLGRIVELAPAATVFESSRHPYTRALGLSRLDTDFAKRDFYTLPGEIP
                     SPVKAPPGCPFAPRCERVTAVCTTAFPPQSGDTQSQGEYYCYNPVTP"
     gene            complement(3432..4418)
                     /locus_tag="PLF85_12845"
     CDS             complement(3432..4418)
                     /locus_tag="PLF85_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20606.1"
                     /translation="MKPIKLRLLLWLLASLLLVEGLGAVWTSPAARTEADKDNPFVSG
                     TLRLQLRYKDWLPSLETNYRYETATQGNMRTDGAQNQTAAGLSSFQQQPWHAMLGSYY
                     NILNSLMVGGFYRYAQGERHRNDWVGSGWAGSGSTTWDWFWLNTNSRPEHSAIGDITF
                     RYRLGFLPGENWVFELKNRAIYTWYTDARYASRDNWGQHSDAVAETKYVVRPGLQFFW
                     LDGDRPFLTLFAQYEAHFALNFGTRRLVESWAYLGFLYHMSEEVALGMNLAFAQWWWS
                     ESDSVRNIRAAETCTAGASAAFTCNTTQYVTTQRAFIVGAIALFRFDLTPIE"
     gene            complement(4482..>4739)
                     /locus_tag="PLF85_12850"
     CDS             complement(4482..>4739)
                     /locus_tag="PLF85_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20607.1"
                     /translation="AYYPIEIGRGFRLLGSISTGPNVFFVLSSAASGTAVQWGVMPSV
                     ELQYQLWERTSLYVGSGINFTFDPNGILRFVPMSFGITRHF"
BASE COUNT         1096 a         1354 c         1311 g          978 t
ORIGIN      
        1 agtgctgaaa accgtcatcg agcagaaaga gatcaacacc gtgttcttca accagccgtt
       61 gcgcattgcg caaccggttt ctgcccaccg ccacgcgcac accgggcagg ttcactgcca
      121 tcaggtacgg ctcgtcgccc gaatcatcgc tgctcatcag caggcgtacc ccgtcagaaa
      181 gcaggttggt ttctttcgaa agtttaccgc cataaccgcg gctcagcacc gcctggttgg
      241 gaaactccga ccgcaagagc cggctgaaat agatcgtggc aggtgtcttg cctgtgccgc
      301 cggtcgtgat attgccgacc gacagaattt tcgccccagg aatacgaatg ctgcgcgtca
      361 tgcggcggtg cagcctgttg ccgaaggaat aaagcttttc gagaggattc atctgtgctg
      421 tcactacccg tagatggtga caaagccatg gcgtcgagcg gtttgcctgc atatggcttt
      481 ttgcctgctg ctgttcgcct cacccacaga ccagttctgg atgggccagc agcctgacga
      541 caccacagcg ttcaggctac tgaccgactt tcacctgaag accattgccc ccgaccgcgg
      601 ctacctgcac caggtgagcg cgcgcatgac acccgtgcac ccggcaaccg acaagaaggg
      661 caacgtcaaa gaggcatttg aaaaagatca ggatctggtg aaacgctcgg ctgccgcaaa
      721 aagcgctgca gcgtcaggca cacctgcctc ggcagaatac cgctggcacg tgcattcacc
      781 cggcaaggat gtgctctacc ttaaaccggc gcgcgagata tacctgccag accgcaacag
      841 gatgttcgcc gtttatctgc gcggcgccgg gcatgcgcac caggtttatg caatctttcg
      901 cgggcctaac cgtatacagc aggaaatttt tgtctgctcg ctcgactacc agggctggaa
      961 acgttttgaa gtcgtgatac ctccctacct gcagctgcgc aacccgctga agcagaaccg
     1021 gtacgaactc tacttcaccg cactcaaggt gcagagccac tttcgcgacc aacccggaac
     1081 gtcagtcttc aacctcgcgg agatgttcat catggctgac acatcggaca agaagatccc
     1141 cggcgccgat atgccacagg aattctagag agattaatag actgcggcgg tgccccagtc
     1201 tattaatcca ggttaatatc cgtcagcagc ttttgttcgt cggcaccgag gtcgagctgt
     1261 tcaatttcga tatagtcggg cttgaatata tgcaccaatg actgcagcac caggttggaa
     1321 tcgaggtacc agacgatgcg gcggggttcg aatgccacgt tgtggatctt gatcttgccg
     1381 agtatgtcga cctcgggcgg tatctgcatc aggatgaagt tgagatcgaa gccgaagaaa
     1441 cgcttcttct tattcaccat gaaaaaaggt gaatcgccgc gcgtgaggtg gtagagaata
     1501 atatcttcca gtttgctcga gaaccgtgtg ctcaggcgaa tcatatccgg cttgcgcgag
     1561 ctgaagaatc gtgatttcgt gaggcgcatc ttgaccgtat gtgccgtcac ctcttctttc
     1621 ttcagctgga agtcgaaata gatctgaccg gttttgagac caatgacgcg ctcatgacct
     1681 tcgcgtatca caccgcgcag gtgcagcgaa cgattggtgc ataaaagccg cagactgacc
     1741 aggcggtcat aatgcacaac gacgtggtcg cgcaagaggt cattgaggac tacctgccgt
     1801 gtgaccgctt tatacatgca ttaagctcag gtattaaaat agcggtcacc ggcatcgccg
     1861 aggcccggca tgatataacc cttgttgttt agcctttcat caacaaccgc cgtgtagagc
     1921 ttcacatccg ggtgcgcctt ctcgaccagt tgaatgccct cgggtgcggc aatcacgctg
     1981 atggcgcgca gcgtacgcgg tttgccctgt ttgagctttg tcagcgcaaa atccagcgaa
     2041 ccacctgtgg ccagcatcgg gtcaagcacc cagacgtcgg cattcttcag cgagggagtt
     2101 ttgtagtaat aaaactttgg cttgagcgtc tcgtggtcgc gttccacacc gacatggccc
     2161 gtggccgcat cgggcagcag ggtgcgaaag ccctcaagca tgccaaggcc tgcgcgcaag
     2221 atcggtatca gtaccaccgg gctcttcacg acacgccccc gcgttttcac caggggagtc
     2281 gcaacagtcc ttggtatcgt ctccatttcg ctcgcaacga gcgcgcagag tatcaggcta
     2341 atctcgtgca aggtgcgcgc gaaaagcggc gaaggagttt tcttgtcgcg caatacggaa
     2401 atttttgcgg acagcagcgc gtggttggcg tagtttttag ctttcatggt gtaaccggat
     2461 tgtaacagta gtactccccc tggctttgcg tatcgcctga ttgcggggga aaagccgttg
     2521 tacaaacggc agtgacccgc tcgcagcggg gggcaaacgg gcagccaggc ggagctttga
     2581 ccggcgaggg aatttcaccg ggcagggtat aaaaatcgcg tttcgcgaag tccgtgtcga
     2641 gccggctcaa gcccagcgcc cgcgtgtaag ggtggcggct gctttcaaag accgtggcag
     2701 cgggggccaa ttcgacaatg cggccaaggt acataacagc aacccggtcg gaaaaccagc
     2761 gcactttaga aaggtcatgc gtgataaata acgctgcgaa accgtactgc gcgtgcaggt
     2821 ctttcagcag gttgagaatc tgcgcctgta tcgaaacatc gagcgcgctg atgggttcat
     2881 cgagtataag gtaacggggc ttgagtacca gcgcgcgcgc aatggcaata cgctgccgct
     2941 ggccgccgct gaattcgtgt atcttgcgcc ccatggtttc agccggcatt cccgtagcgg
     3001 caagaatttc tgccacccgg tcacggcgcg cggcgcgtgt caggtccagt tttttcttcc
     3061 tgatcaccat cggctcggcg acgatctgct cgacagtcat ccacgggttc atcgaagacg
     3121 cgggatcctg gaaaacaata ccgatgtcgc gccgcaggtt ctgccagtcg tcgcgtttca
     3181 tgctgcggct atcgtaaccg tcaaaggtaa agtgcccttc atcgggccga tagaggccca
     3241 tgatgacgcg ggcgagcgtg ctcttgccac tgcccgactc gcccacgaga ccgacaattt
     3301 ctttctcgcg gatttcgagc gagacgtttt gcagcgcgcg taccgtctgc ctgtcacgcg
     3361 acaggaaacc gcggtttacc gcgaaaaatt tagaaactcc gttgagcgcg atcatgcgta
     3421 tagggtctgc actattcgat cggcgttaaa tcgaaacgaa acagcgcaat tgccccaaca
     3481 ataaaagccc gctgtgtcgt cacgtactgc gtggtattgc aggtaaaggc agctgatgca
     3541 ccggcagtgc aggtctctgc cgcgcgaatg ttgcgcacag aatccgattc gctccaccac
     3601 cactgcgcaa aagccaggtt catacccagc gccacctctt cgctcatgtg atagagaaag
     3661 ccgagatatg cccagctttc gacaagccgc cgggtgccaa agttcagagc aaaatgcgct
     3721 tcgtactgcg cgaaaagggt caggaacggc cggtcaccgt cgagccagaa aaactgcagg
     3781 ccggggcgca cgacgtactt cgtttcagca accgcatcgg agtgctgccc ccagttgtca
     3841 cggctcgcgt aacgtgcatc ggtataccat gtgtagatcg cgcggttctt gagctcgaaa
     3901 acccagtttt cccccggcag aaaacccagc cggtagcgaa aggtaatatc accaatggcc
     3961 gaatgttcgg gccggctatt cgtattcagc cagaaccaat cccaggtagt cgagccgcta
     4021 cccgcccagc cgctgcccac ccagtcattg cggtgcctct cgccctgcgc atagcggtaa
     4081 aaaccgccaa ccatgaggga attcaggata ttataatagc tccccagcat cgcatgccac
     4141 ggctgctgct gaaaactgct tagcccggca gcagtctggt tttgcgcgcc atcggtgcgc
     4201 atattaccct gggtggcagt ttcataacgg tagttggtct cgagcgacgg caaccagtcc
     4261 ttgtagcgca gctgtagtcg cagcgtacca gagacaaaag gattgtcttt atcggcttca
     4321 gtgcgcgcgg ccgggctcgt ccacacagca cccaggcctt cgacaagtaa caggctggcc
     4381 aacaaccaga gcagcaggcg gagtttaatc ggtttcataa ggcccgaaac aaagagaggg
     4441 gcatcgcccc atcagacggt caagccaatt cggcgacaaa ttcagaagtg gcgcgtgata
     4501 ccgaaactca tcggcacgaa gcgcagtatt ccattgggat caaaagtgaa attgatgcct
     4561 gaacccacat aaagcgaagt gcgctcccac agctggtact gcagttcaac cgaaggcatg
     4621 actccccatt gcaccgcggt gcccgatgct gcagaagaca gcacgaaaaa aacattgggg
     4681 cctgtagata tagaacctaa caaacgaaaa ccgcggccga tttcaatggg gtagtacgc
//
LOCUS       DAOQJF010000267         4636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11017_length_4636_cov_4.540712, whole genome shotgun sequence.
ACCESSION   DAOQJF010000267 DAOQJF010000000
VERSION     DAOQJF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11017_length_4636_cov_4.540712"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLF85_12855"
     CDS             complement(<1..929)
                     /locus_tag="PLF85_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNE20608.1"
                     /translation="MNSIKITGAREHNLKNISLEIPREKLVVITGLSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLGQLEKPDVDSIEGLSPAISIEQKTTARNPRSTVGTVT
                     EIYDYLRLLFARLGEPHCPECGARLEAQSIDTMVAHTFGIFDRIPAAKSVRTQILSPI
                     ARSKKGEFKDVFERIQREGFVRVRVDGRIISLDEPIELKKNLKHDIDIVVDRLVLEKE
                     NLQSQRMHLAGSLELALKQATGGGQASILFETDNGQSIEENYSAKLYCSACDLSFPEI
                     THRLFSFNSPDGACENCSGLGHMLEFHPDRLIR"
     gene            complement(972..1820)
                     /locus_tag="PLF85_12860"
     CDS             complement(972..1820)
                     /locus_tag="PLF85_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HNE20609.1"
                     /translation="MRKMYDEYFATTRFADLDTQRHVTSRTYEEFALEGRYRVLAKLG
                     YNQERIQNENIYLEAISGYSKFHRQQFPGVQLKVESFAIACGDGIIHWDQKIKDPAGE
                     LVCHLQLKTRTTKGGAALELEGIETAENFEVLYSDLEPWNGSNERIVSHYTMPYSDRD
                     FAGRYNVASLWRIFEEGRWMFAEQTGMTYERIVEMDTTSFYMGSICNYFAEVPAGRTL
                     QIMTWIERVEKIRYYFRQDVVLNGKVLLSMRDEQLIVSLSRARPQRASEKFLGFIGKY
                     LEHGPG"
     gene            complement(1813..3033)
                     /locus_tag="PLF85_12865"
     CDS             complement(1813..3033)
                     /locus_tag="PLF85_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE20610.1"
                     /translation="MYRFLVTASILVLLGFIYLGQRLIPPIWPVAVRTTLWLLLAAMI
                     LNLLWLPFKRWRISGKSRANMDAPGLATRLAEASAYTFMGFLSLLLFFTVSGEFVRIV
                     FSLMPQMLQGDLLVLIPAGQNLDSVLSLLIMASALLFFFWGRFSVALGPRYRFVRVVL
                     PLHGKLRRRPRFRRSAQATHKLRLVQVSDLHIGRAIKEGYVRKVVEMINAQQADLACF
                     TGDIGDGNIAELRSDAAPLADIQTRHGVYYVPGNHEYYWGMTEWIKEFTALGMKAVIN
                     RGETIQTKDLSLFVAGVSDPTAARFAGELPKIPKPEKTNFSLLLVHQPLFARSAASLG
                     YDLMLAGHTHGGQYIPWSWVVHLFFRFAKGLYRTGNMLVYVNDGTGYWGAPLRIGTRP
                     EITVLDIYFSEVGA"
     gene            3113..3844
                     /gene="rnc"
                     /locus_tag="PLF85_12870"
     CDS             3113..3844
                     /gene="rnc"
                     /locus_tag="PLF85_12870"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNE20611.1"
                     /translation="METSNSEQERQAKLADFLRQEGLPDKVLPALNVALTHKSYSNEF
                     RSGFDPGMDKHNQRLEFLGDSVLGLVIAHHFYVSKRKTAEGGLTRLKAMTVCESALKK
                     VADKLQIGQLLRMGKGEIGTGGLTRASNLADAMEAVIGAIFITTDFETAQKFVVRHFR
                     DILDHPETVEGSKDHKSALQELLAKRSHQRPDYQLVAAEGPDHDKEFTVALYVAGEKK
                     SEGVARTRKQAEQNAARAYLEKHGK"
     gene            3942..>4636
                     /locus_tag="PLF85_12875"
     CDS             3942..>4636
                     /locus_tag="PLF85_12875"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HNE20612.1"
                     /translation="MRFGRSSPVLQGGVIALLSLFCEINAAVSPQGVYTHAIPISIPP
                     ALAGVGPEDLSLVYNSGNGSSSIAGVGWSLTGIPMIGRAKGMHYHGQDSYHIGGQKLL
                     RQPNGNYSAGTTGFIPQLGDCGLPENEPCSWLGKPLNGTTIHFGSGSNSRASAAGKSG
                     YRVWAADRIENSAGLYYTIEYLASGGELYPERITYTFGPTSQIYRRVEFFYEDEPQPH
                     IILSDGVPVSLNKR"
BASE COUNT         1056 a         1231 c         1300 g         1049 t
ORIGIN      
        1 tcgcgtatca gccggtcagg gtgaaactcc agcatatgac ccaggccact gcagttttcg
       61 caggccccgt cgggggagtt aaaggagaat agacggtgag tgatctcagg aaaactcagg
      121 tcgcaggctg agcagtagag ttttgccgaa taattttctt cgattgattg cccgttgtcg
      181 gtttcgaaca gtatactcgc ctgtccgccc ccggtcgcct gcttcagcgc aagttcgagc
      241 gaaccggcga ggtgcattct ctgtgactgc agattttctt tctcgagtac caggcggtcg
      301 accacgatat caatgtcgtg cttcaggttc ttcttgagtt caatgggctc atcgaggctg
      361 atgatgcggc catcgacacg cacgcgcacg aaaccctcac gctgtatacg ctcgaacacg
      421 tctttaaact cgcctttctt gctgcgcgcg atgggtgaga gtatctgcgt acgcaccgat
      481 tttgccgcag gtatgcggtc aaagataccg aaggtgtgcg cgaccatggt gtcgatcgac
      541 tgagcctcaa ggcgcgcccc gcattcggga caatggggtt cgccgagacg ggcaaaaagc
      601 aggcgcaggt agtcgtaaat ttccgttacg gtgccaacgg tcgagcgcgg gttgcgcgcc
      661 gtggtcttct gttcaatcga gattgcaggc gaaagcccct cgatggaatc gacgtcgggt
      721 ttttccagct ggcccaaaaa ctgccgcgcg tatgctgaca gcgattcgac gtagcgccgc
      781 tggccttcgg cgtagatggt gtcgaaggcg agtgagcttt taccgctgcc cgaaagaccc
      841 gtgatgacaa ccagtttctc gcggggaatt tccagcgaaa tattcttcag gttgtgttcg
      901 cgcgcgccgg tgatcttgat cgagttcatg ggtgtctctg cctgcgttaa atcaaataga
      961 ttactcgttc actatccggg gccatgttcg aggtactttc cgataaagcc caggaacttt
     1021 tccgaggccc gctgcggtct tgcccttgac agcgaaacga tcagctgctc gtcgcgcatc
     1081 gacagcagta ccttcccgtt gagcactacg tcctggcgga aatagtagcg tatcttttca
     1141 acgcgctcaa tccatgtcat gatctgcagg gtacggcccg ctggtacctc ggcaaaataa
     1201 ttacagatgc tgcccatgta gaaagacgtt gtatccatct cgacgatccg ttcgtacgtc
     1261 atgccggtct gctcggcgaa catccagcgg ccttcttcaa agatgcgcca gagcgaagcc
     1321 acgttgtagc gccccgcgaa atcacggtcg gaatacggca tagtgtagtg gctcacgatg
     1381 cgctcgttgc tgccgttcca gggttccaga tccgagtaga gtacttcaaa attttccgca
     1441 gtttcaatac cttcgagttc aagcgcagct ccccctttgg tcgtgcgggt ctttaactgc
     1501 aggtgacaga cgagttcgcc ggccgggtct ttgatttttt gatcccagtg aattattcca
     1561 tcgccgcagg cgatggcaaa gctttccacc tttagttgta cccccgggaa ctgctggcgg
     1621 tggaatttgg aataccctga aatcgcctcg agatagatat tctcgttttg gatacgttcc
     1681 tggttatagc caagtttcgc cagcacccgg tagcgaccct cgagtgcgaa ctcttcgtac
     1741 gttcggctcg tgacgtggcg ctgcgtgtcg aggtcagcaa aacgtgttgt ggcaaagtat
     1801 tcgtcataca ttttacgcac cgacctcgct gaaataaata tcgagtaccg taatttcggg
     1861 ccgcgtgcca atacgcagcg gagctcccca gtagcctgtg ccgtcattca cgtagacgag
     1921 catgttaccg gtgcggtata ggcccttggc aaagcggaag aacaggtgca ccacccatga
     1981 ccagggaata tattgtccgc cgtgtgtgtg cccggcgagc atcaggtcgt aaccgagcga
     2041 cgctgcactg cgcgcgaaca gcggctggtg aacaagtaac aaagagaagt tggttttttc
     2101 cggtttcgga attttcggca attcaccggc gaagcgcgcc gctgtcggat cgctcacgcc
     2161 tgcgacaaaa agcgaaaggt ccttggtctg gatggtctcg ccgcggttga tgaccgcttt
     2221 cataccgagc gcagtgaact ctttgatcca ttcagtcatg ccccagtagt actcgtggtt
     2281 gccgggtacg tagtaaacac catgccgcgt ttggatgtcg gccagtggtg cagcatcaga
     2341 cctgagttcc gcgatgttgc cgtcaccgat gtcaccggtg aaacaggcga gatcggcctg
     2401 ctgcgcattg atcatctcga ccaccttgcg aacataacct tctttaatcg ctcggccgat
     2461 gtgaaggtcg ctgacctgca caaggcggag cttgtgtgta gcctgcgcag acctacgaaa
     2521 gcgcggccgg cgtcgcagtt tgccgtgcag gggcaatacg accctgacaa aacggtaacg
     2581 tggtcccagc gcgacgctga atcgacccca gaaaaaaaac aggagtgctg atgccatgat
     2641 cagcagcgac aaaacagaat cgagattctg ccccgcgggt attaagacca gcaggtcgcc
     2701 ctgcagcatc tgcggcatta agctgaaaac gatgcgaaca aactcgccgc tcacggtaaa
     2761 gaatagcagc aggctcagaa atcccatgaa ggtgtaggcg ctcgcctctg cgagccttgt
     2821 cgccaggccg ggagcatcca tgttggcgcg cgattttccg ctgatccgcc agcgtttaaa
     2881 cggtagccac agcaggttca ggatcatggc agccagcagc agccacaggg tagtgcgcac
     2941 ggcgactggc cagatgggtg gtatcaaccg ctggccgaga taaatgaagc cgagtaaaac
     3001 taaaatagac gcggtgacta agaagcggta cattgtagtt ggatgtcact taacgtttac
     3061 tgcggaattt tcgcagcaaa cgttaaatga cgatccacgg cacggagact gcgtggaaac
     3121 gagtaattca gaacaagaac gacaggcgaa gctggcagat tttctgcgac aagagggctt
     3181 acccgataag gtattacctg cgctcaatgt cgcgctgacg cacaagagtt attcgaacga
     3241 atttcgctcc ggttttgacc cgggcatgga taaacacaac cagcggctcg aattcctggg
     3301 agactcggtt ctgggtctgg ttatcgcgca ccatttttat gtcagcaaac gaaagaccgc
     3361 cgaaggcggg ctgacgcggt taaaagcgat gaccgtctgt gagtcggcgc tcaagaaggt
     3421 cgcggataaa ctgcagattg ggcaactgct gcgcatgggc aagggcgaaa ttggcaccgg
     3481 gggcctgacg cgcgcttcca acctcgccga tgccatggaa gccgtgatcg gcgccatctt
     3541 catcaccacc gattttgaga ccgcgcagaa atttgttgtc cggcattttc gcgatattct
     3601 cgatcaccct gagaccgtcg agggcagcaa agatcataaa tccgcgttgc aggagctgct
     3661 agccaagcgc agccaccagc gtcccgatta ccagttggtt gcagccgagg ggcccgatca
     3721 cgacaaagaa tttaccgtgg cgctctatgt cgcgggcgag aaaaaatcag aaggtgtcgc
     3781 gcgtacacgc aagcaggcag aacagaatgc tgctcgggcg tacctcgaga agcatgggaa
     3841 atagataaca ggcgtttact ttttccgcga gactttacag ttaatttctt tacgcgctgt
     3901 tttagcttcg gtcgaagccg gttttagcat tgaggatcat catgagattt ggccgatcat
     3961 ctcctgtact acagggcggg gtaatcgctc tgctatcatt gttctgcgag atcaacgcag
     4021 cagtaagccc gcagggcgta tatacccacg cgattcccat ttctattcca cccgcacttg
     4081 cgggtgtagg tcctgaagat ctttcactcg tttacaacag tggtaatggc agcagcagta
     4141 ttgctggcgt aggttggtcg ctcaccggaa ttccgatgat cggtcgggcg aagggtatgc
     4201 attaccatgg tcaggatagc tatcacattg gtggacagaa acttctgcga cagccgaacg
     4261 gcaattattc ggcgggtacc acggggttta tacctcaact tggcgattgt ggtctgccag
     4321 agaatgaacc ctgtagttgg ttgggcaaac cactcaatgg tacgaccata cattttggtt
     4381 cagggagcaa cagccgcgcg tctgcggccg gaaagtcggg ttaccgggta tgggccgccg
     4441 acagaatcga aaattctgca ggcctttact ataccatcga atatctggca tcgggtggcg
     4501 aactttatcc ggagcgcatt acgtacacat tcggtccgac cagtcaaatt taccgtcggg
     4561 ttgagttctt ctacgaagat gaacctcaac cgcatataat cctcagcgac ggtgtaccgg
     4621 tcagtctcaa caaacg
//
LOCUS       DAOQJF010000268         4636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11019_length_4636_cov_3.879284, whole genome shotgun sequence.
ACCESSION   DAOQJF010000268 DAOQJF010000000
VERSION     DAOQJF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11019_length_4636_cov_3.879284"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..441)
                     /locus_tag="PLF85_12880"
     CDS             complement(19..441)
                     /locus_tag="PLF85_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE20613.1"
                     /translation="MKTVYLDSSAVLRWLLKDRNALKDFGQWKHAGSSFLLEVECIRT
                     LDRLRMENRIDAGLYADLLAQLNEFSRSLELFHIDARVINQAKSQFVAPVKTLDAIHI
                     ATAQLWQDELSETVLLISHDQKMNLIAKSLGLKTLESK"
     gene            complement(422..688)
                     /locus_tag="PLF85_12885"
     CDS             complement(422..688)
                     /locus_tag="PLF85_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNE20614.1"
                     /translation="MASSTAAVSEFKAHLSEYLREVQNGHEITILDHKRPVAKVVPIA
                     NETMRDRLGSRPPLQKGFRDIRPHVELDIDPAQYIIEDREDRLP"
     gene            complement(759..3575)
                     /gene="ileS"
                     /locus_tag="PLF85_12890"
     CDS             complement(759..3575)
                     /gene="ileS"
                     /locus_tag="PLF85_12890"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNE20615.1"
                     /translation="MNKDKENKPKNPWEHTIALPQTVYPMRADLAKRENDILAIWKKE
                     NLFQKINAKRKAENAPLFLLHDGPPYANGEFHTGHGLNKVLKDIFNKYNLLRGRRVPY
                     VPGWDCHGLPIELAAIKKLANKKDGSDKDPIRIRKACREYAAEYIKIQGEDQTRFGIF
                     WDDSDHKTAANNESKNYYATMSPKYEAAILKAFRDLFLKDLIYKGKKPVYWDATTASA
                     HAEAEIEYQEHESDAIFVKFPIRNRSNAYVVIWTTTPWTLPANLGVCFSESIRYVAVK
                     TDAGELILAADLVESVLKAAGLENKGQSDINLDEIQKLEVAHPFIERASKVLFGDHVT
                     ADAGTGIVHTAPGHGQDDYNVGLKYGLEILSPVDHRGRYTKEYAEMEGKSVFEANPLI
                     IEKLKESGLLLAHERFKHQYPHSWRSHKPLIMRATPQWFMKIEPLRELALSEIPKIEW
                     IPEWGESRFTAAVKTRPDWCLSRQRYWGVPIPAFQCKQCGHVHMDDKSLGHVIEKVEA
                     QGIEVWYEKPAAELLPAGTKCTECGSTDFEKESDILDVWFDSGVSWYAVLKDNPELAY
                     PADVYLEGSDQHRGWFQSSLWPALALTGRAPYKKVVTHGYILDQQGRAMSKSLGNGMS
                     PRADIINKFGADILRLWVSSEDYRTDNKIGPEMINHLGDAYRKIRNTLRYLLGNTQDG
                     NASTTLSDQHITQKLDKWVLAEAAELVKQVTDAYDKSEFHLVYHRTLQFCTVTLSNLY
                     LDMVRDSLYCDTDPRRGSTPLPKRETEDGRTEPGENRALSEAEGRRNSTLRTLQILTE
                     TLVTLLAPVLSFTAEETQRVLTPDHSVFENPWPDLTRFANADLIKEFEQLKQVRAEVN
                     TAIEPMRKAGEVGSDVEVEVEIPQQADEGTLLRFLGVSSVKTGASALKVRKSAKPKCP
                     RCWLHRDLIASGLCERCDSVVSGS"
     gene            3830..4198
                     /locus_tag="PLF85_12895"
     CDS             3830..4198
                     /locus_tag="PLF85_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE20616.1"
                     /translation="MTKYFLLLTSAALITACGKKPEEAPAPAAAAAVSGEAVYNEKGC
                     VTCHGAQGKGDGPVGAALKPKPRNFADAKWKNGTELAAVIKSIENGIPGSGMTAYKGV
                     LTDEEIKAVAEHVRKLGGKN"
     gene            4226..>4636
                     /locus_tag="PLF85_12900"
     CDS             4226..>4636
                     /locus_tag="PLF85_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE20617.1"
                     /translation="MDPRLFKTIKYGTPVVLVAFAAWIIWAPPARVGYAPEQPISYRH
                     DIHAGDPAKKTFDGREQYGIDCRYCHTGVEVSKKAGVPSMNTCMNCHISVGYGKPGVE
                     KLKKFYNDKKPLEWVRVHNLPDHVRFPHAPHIKQL"
BASE COUNT          955 a         1239 c         1317 g         1125 t
ORIGIN      
        1 tgataaagat tttctgtttc attttgattc cagtgttttg agtccgaggg atttagcgat
       61 gaggttcatt ttttgatcat gagaaatcag caggactgtt tcggatagct catcctgcca
      121 caattgcgcg gtcgcgatat ggattgcgtc aagagttttt acgggggcga caaattgact
      181 ctttgcctgg ttaattaccc gggcgtcgat atgaaatagt tcgagcgaac gggaaaattc
      241 gttgagttgt gcaagcaggt cggcgtatag tccggcatca atcctgttct ccattctcag
      301 ccgatcgagc gtgcggatgc attctacttc cagcagaaag cttgagccag cgtgcttcca
      361 ttggccgaaa tccttaagcg cattcctgtc ttttagcagc cagcgcagga ctgctgacga
      421 gtcaaggtag acggtcttca cgatcttcga tgatgtactg ggcaggatcg atatccaatt
      481 ctacatgtgg tctgatatcg cgaaagcctt tctgaagcgg gggacgtgag cccagccgat
      541 ccctcattgt ttcattcgca atcggcacga cttttgcgac cggacgcttg tggtccagta
      601 tggtgatttc atggccgttc tggacctcgc gcaggtattc gcttaagtgc gctttgaatt
      661 ctgagactgc agcggtcgaa ctggccatta ctgtagttta ggtcataatg gtcagattgt
      721 caattgttat tgtggtagcg atgcaagctc ttgtggggtt acgacccgct gacaacactg
      781 tcgcaacgct cgcacaaccc gcttgctatc aggtcgcggt gcagccagca gcgcgggcac
      841 ttgggcttcg cggatttacg caccttcagg gcactggcgc cggtcttgac gctcgaaacc
      901 ccgagaaagc gcagcagcgt gccctcgtcg gcctgttgcg gtatttcgac ctcaacttca
      961 acgtctgagc cgacttcgcc ggccttgcgc atcggttcaa tggcggtatt gacctcggcg
     1021 cgaacctgct ttagctgctc gaattctttg atgaggtctg cgttggcaaa gcgcgtgaga
     1081 tcgggccatg ggttctcaaa tacgctgtgg tccggcgtca gcacgcgctg cgtctcttca
     1141 gcggtgaacg acagtacggg cgccaaaagc gtcaccagcg tttcggtgag aatctgcagc
     1201 gtccgcagcg tggagttgcg gcgcccttcg gcttcgctca gggcccgatt ttccccgggc
     1261 tcggtgcgac cgtcttcggt ctctcgtttc gggagcggag tcgaaccgcg ccggggatcg
     1321 gtgtcacagt acaaggaatc ccgcaccatg tcgaggtaca ggttcgacag cgtcacggtg
     1381 cagaattgca gcgtacggtg gtagacgagg tggaactccg acttgtcgta ggcgtcggtc
     1441 acctgcttca ccagttcggc cgcttcggcg agcacccatt tgtccagttt ctgggtaata
     1501 tgctggtcgc tgagcgtagt cgaagcgttg ccatcctgtg tattgcccag gaggtagcgc
     1561 agtgtattgc gtatcttgcg gtacgcgtcg ccgaggtggt tgatcatctc ggggccgatc
     1621 ttgttgtcgg tgcggtagtc ttctgaagac acccacaggc gcaatatatc ggcgccgaac
     1681 ttgttgatga tgtcggcacg cgggctcatg ccattgccca gggacttgga catcgccctg
     1741 ccctgctggt cgagaatgta gccgtgtgtc acgaccttct tgtacggggc gcgcccggtg
     1801 agcgccaagg ccggccacag cgaagactgg aaccagccgc ggtgctggtc agagccctcg
     1861 aggtagacat cggccgggta agcgagttca gggttatctt tgaggactgc ataccagctg
     1921 acgccgctgt caaaccagac atcgagaata tcgctctcct tctcgaagtc ggtcgagccg
     1981 cattcggtgc atttggtgcc cgcaggcaaa agctccgccg caggcttttc ataccacact
     2041 tcgatgccct gcgcttcgac cttttcaatc acgtggccca agctcttgtc gtccatatgg
     2101 acgtggccgc actgcttgca ctggaacgcc ggaatcggta caccccagta ccgctggcgg
     2161 cttaagcacc agtcgggccg ggttttgacc gccgcggtga agcggctttc gccccattcg
     2221 ggaatccatt cgattttggg aatttcactg agcgctagct cacgcagcgg ctcaatcttc
     2281 atgaaccact gcggcgtcgc gcgcatgatc agaggcttgt gcgaacgcca cgaatgcggg
     2341 tactggtgct tgaagcgctc gtgcgcgagc agcaatcccg attctttgag cttttcgata
     2401 atgagcgggt tcgcctcgaa cacactcttg ccttccatct cggcatactc tttcgtgtag
     2461 cggccgcggt ggtcgacggg cgaaaggatt tcgaggccgt acttgagccc gacgttgtag
     2521 tcgtcctggc catggccggg agccgtatgt acaatgccgg taccggcgtc tgcggtcacg
     2581 tggtcgccaa acagcacttt agaggcacgc tcaatgaatg ggtgtgcgac ttccagcttt
     2641 tgtatctcat ccaggttaat gtcggattgt cccttgttct caagccctgc tgccttgagc
     2701 acgctttcga cgaggtctgc cgcgagaatc agctcgccgg cgtcggtctt gaccgccacg
     2761 taacggatgc tctcgctgaa gcacacgccg aggttcgccg gcaatgtcca tggggtggtc
     2821 gtccagatca ccacgtatgc attggagcgg ttcctgatgg ggaatttaac gaagatggcg
     2881 tccgattcgt gctcctggta ttcaatctcc gcctcggcgt gggctgacgc agtcgttgcg
     2941 tcccagtaaa ccggcttctt tcctttgtag atcaggtctt tcagaaagag gtcacggaac
     3001 gccttgagta tcgccgcctc gtatttgggc gacatcgtgg catagtaatt cttcgattcg
     3061 ttgttggctg cagtcttgtg gtcgctgtcg tcccagaaga tgccaaaccg tgtctggtct
     3121 tcaccctgga tcttgatata ttcggcagcg tactcgcgac aggccttgcg tatgcggatc
     3181 gggtctttgt ccgaaccgtc tttcttattc gcgagttttt tgatcgcggc gagttcaatc
     3241 ggcaggccat ggcaatccca cccggggaca tagggcacgc ggcggccgcg cagcaggttg
     3301 tatttattga agatgtcttt gaggaccttg ttcagtccat ggccggtatg gaactcgccg
     3361 ttggcgtaag gcgggccatc gtgtaataag aagagaggcg cattttcagc cttgcgcttc
     3421 gcgttgatct tctggaacag gttttctttt ttccagattg ccaaaatgtc gttctcgcgt
     3481 ttcgccaggt cggcccgcat ggggtagaca gtctgcggca aagcaatggt atgctcccac
     3541 gggttcttcg gtttgttctc tttatcttta ttcatgtggc tcttaaagga agaagctgga
     3601 aaaagaaaca ggctgcacag tcaatcttaa gcaacaggta gaaattggcg ggcggatagg
     3661 cctcatattg tgaccactct ttaaccgcca aagataatta ggagaaaagg actcagccgg
     3721 tcttttttgt gccttgtcag cgttttgtca aaaaaaaatc gacgtgctat ttcgaatcga
     3781 aacatgagcc aaccggcaaa atactcgatt tttgccaaag gagcacaaca tgacaaaata
     3841 ttttcttctt ctcacctcag ctgcactgat cacagcctgc ggcaaaaaac ctgaggaagc
     3901 acctgctccg gcagctgcgg cggccgtaag cggtgaagcc gtttataacg aaaaaggctg
     3961 tgtaacttgc cacggtgcgc aaggcaaagg cgacggccct gtaggcgcag cgctcaagcc
     4021 aaaaccccgc aattttgctg acgcgaaatg gaagaacggc acagaactcg cggcagtcat
     4081 caagtctatt gaaaatggca ttccgggctc aggcatgact gcgtacaaag gcgttctgac
     4141 tgacgaagaa atcaaggctg ttgctgagca cgtgcgcaag ctcggcggca agaactaaag
     4201 gcaatcactg aggagctcaa gtccgatgga cccaagactg tttaaaacaa tcaaatacgg
     4261 aacaccggtt gttctcgtcg catttgcggc ctggattatc tgggctcctc ctgctcgtgt
     4321 tggctatgcc cccgagcaac cgatttcata tcggcatgac atccatgccg gcgacccggc
     4381 taaaaagacc tttgacggcc gcgagcagta cggcattgat tgccggtatt gccacacggg
     4441 cgtcgaggtg agcaaaaagg cgggtgtgcc cagcatgaat acctgcatga actgccacat
     4501 ttctgtcggt tatggcaagc ctggcgttga gaagctgaaa aagttctaca acgataagaa
     4561 gccgctggaa tgggtgcgcg tccacaacct gcctgaccac gtacgcttcc cccacgctcc
     4621 acacattaag caactg
//
LOCUS       DAOQJF010000269         4583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11214_length_4583_cov_4.797703, whole genome shotgun sequence.
ACCESSION   DAOQJF010000269 DAOQJF010000000
VERSION     DAOQJF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11214_length_4583_cov_4.797703"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLF85_12905"
     CDS             <1..436
                     /locus_tag="PLF85_12905"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF020460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNE20618.1"
                     /translation="KSDHGTYGMAMLSFDSAGEILEINRKEKNKLFKGKSSVVVKDYL
                     LQEGVPTIVREADHFSETVVMLANNQFIGCFARANEQKDGRTSLNSTGMYFKPHTLAA
                     TDNDATRVAAIITRVAGIALQREICELVAHGAPGTAEPLPCP"
     gene            439..1389
                     /gene="gshB"
                     /locus_tag="PLF85_12910"
     CDS             439..1389
                     /gene="gshB"
                     /locus_tag="PLF85_12910"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNE20619.1"
                     /translation="MQFAFLIDPPGSLNIKKDTSFALMLEAQAQGHTIWLLERGDLSL
                     QAGKLYANARQARVADDFNRPFELSTPERIPADRFDVLFVRTDPPFNTDYLTDTWLLS
                     HAGNRPLVLNSPEGLRNINEKIWAARFADLTPTTLITADKAEFLAFLQQYQRVVIKPT
                     DGFGGSAVFLLQAGDKNAAVTFETLSRMNRYVIVQEYLPKATEGDKRILLLQGEILGA
                     VLRLHSGDDHRNNFAAGGKPGETTVTAAERAICERLKPFLVENGIFFAGIDVIGEKLI
                     EVNVTSPTCLREMQYYYSENLAAKVIHAAAALAEKSHARN"
     gene            1376..2326
                     /locus_tag="PLF85_12915"
     CDS             1376..2326
                     /locus_tag="PLF85_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE20620.1"
                     /translation="MHAIEIQNLRKTYPLGGSGHGPAGAGFEALKGIDLTVDRGDFFA
                     LLGPNGAGKSTLIGILASLVMKSSGSCRIMGVDMDKDMPRAKSFLGLVPQEFNLNIFE
                     PPMQILVNQAGYYGITPKVARTRAEKYLTQLGIMEKALTPTRMLSGGMKRRLMIARAL
                     IHEPAILILDEPTAGVDIELRKLIWNFLTELNASGTTIILTTHYLEEAENLCRNIAII
                     DHGQIIAHTAMKKLLSRLNQQTYVIELVKPLAKAPRLSHFQPVRTDNTTLEITVKRNQ
                     PMNALFADFSAKKIGIRSVREKTNRLEQLFLELTGKAGKV"
     gene            2323..3099
                     /locus_tag="PLF85_12920"
     CDS             2323..3099
                     /locus_tag="PLF85_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE20621.1"
                     /translation="MKRADLIGFYTILRKEYIRIVRIWSQTLLPPVMNVTLYLLIFGS
                     FIGKRIGTMGGDSYASFIVPGLILMAVITNSYGNVSSSFFSAKFQKNIEEILVSPIPD
                     WLIVLGFTFGGIIRGALVALCVALVVLIAPGVELRVHNPLLVIVLLLLTAGFFSLAGL
                     MNAMFAKKFDDISFIPTFVLTPLTYLGGIFYPLSALPEFWQRVSAVNPVLHIVNSLRY
                     AFLGHAELPWWYAVVFLLAGNTILYIVCLRYLKKGLGIRN"
     gene            3136..3921
                     /locus_tag="PLF85_12925"
     CDS             3136..3921
                     /locus_tag="PLF85_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE20622.1"
                     /translation="MEILRWQALNPLANYQYIVCDHDRNAALIDPLDTDQVESLASRL
                     SLRIKAILVTHEHPDHSGEAGRLQNAFKVPVYSSHNCAGAVTSARIEGVADKTELRLS
                     KELSFRWHYTPGHTPGHAVIEADGLLFTGDCLFHAGCGHCRSAGANVEDHYASLHTRL
                     AALTPDLLLFPGHYYARRNLDFALHVEPDNRRARDLRHRLANDGDEMNHQTTLRQERE
                     YNPFLRTESRSLRRRVGELSGKDLTEASGLQVFIELRKLRDTW"
     gene            4016..4192
                     /locus_tag="PLF85_12930"
     CDS             4016..4192
                     /locus_tag="PLF85_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20623.1"
                     /translation="MKKLTAIALLAVAMSFVACSSTEEKKAEVPAEAPKAEVKAPAPA
                     AAPAPAKAPAKKKK"
     gene            4271..>4583
                     /locus_tag="PLF85_12935"
     CDS             4271..>4583
                     /locus_tag="PLF85_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNE20624.1"
                     /translation="MTNLTTLLTALEKTAPQAPLQDTIRQLSGSFSNLQIVFSSSLSL
                     EDQAITHIIAEHQLPVRIFTLDTGRHFPETYRTLEATRERYGSEPEVYFPDFAAVERL
                     MK"
BASE COUNT         1093 a         1314 c         1207 g          969 t
ORIGIN      
        1 gaaatcagac catggtactt atggcatggc aatgctctct ttcgacagcg cgggtgaaat
       61 tctcgaaatc aaccgcaaag agaagaacaa actctttaag ggtaaaagtt cagttgtggt
      121 gaaggattac ctgctgcagg aaggcgtacc aacgattgtc cgcgaagcag accatttctc
      181 agagacagtg gtcatgctgg cgaataacca gttcattggc tgctttgcgc gcgccaacga
      241 acagaaagac gggcgcacca gtctcaacag cacgggaatg tatttcaagc cacataccct
      301 ggcagcgaca gacaatgatg ccacgcgtgt cgcagcaatc attacccgcg tggccggcat
      361 tgcactgcag agagaaatct gtgaactggt ggcacacggc gcccccggca cggctgagcc
      421 cctgccctgc ccataaaaat gcaattcgcg ttcctgattg acccgccggg ttcgctgaac
      481 atcaagaaag atacttcctt tgccctgatg ctcgaggccc aggcgcaggg acataccatc
      541 tggctgctcg aacgtggcga cctcagcctg caggcaggaa aactctatgc caacgcgcgg
      601 caggcacggg tggcggacga ttttaaccgt ccatttgaac tcagcacgcc ggagagaatt
      661 ccggcggaca ggtttgatgt actgtttgtt cgaaccgatc cgccattcaa caccgactac
      721 ctgaccgaca cctggctact gtcgcatgcg ggcaaccgtc cgctcgtgct gaacagtcct
      781 gaaggcctgc gcaatatcaa tgaaaaaatc tgggctgccc gttttgccga tctgacaccc
      841 acgacgctga ttacagcaga caaggcggag ttcctggcat ttctgcaaca ataccagcgc
      901 gttgtcatca aaccgaccga tggttttggc gggtctgccg tttttttgct gcaggcgggc
      961 gacaagaatg cggcggtaac ttttgaaacc ctgagccgga tgaaccgcta tgtaatcgta
     1021 caggaatacc tgccgaaggc gacagagggc gataagcgca tactgcttct gcagggtgaa
     1081 atactgggcg cggtgctgcg actgcacagc ggggatgatc accggaacaa ttttgctgcg
     1141 ggcggtaaac cgggagagac aactgtcacc gcagccgaac gcgcgatatg cgaacgcctc
     1201 aaaccatttc tcgtagaaaa tggtatcttc ttcgcgggca tcgacgtgat cggcgaaaag
     1261 ctgatcgagg tgaatgtcac ttcacccacc tgcctgcgcg aaatgcagta ctactacagc
     1321 gagaacctgg cagcaaaggt aatacacgct gccgccgcac ttgcagaaaa aagccatgca
     1381 cgcaattgaa atccaaaacc tgcgcaagac atacccactc ggtggttcag gccacggacc
     1441 ggcgggggcg ggatttgaag cgctcaaggg catcgacctg acagtagacc gcggggactt
     1501 ttttgccctg ctgggaccga acggcgcggg taagtcaacg ctgattggca tactggcatc
     1561 gctcgtgatg aagagcagcg gtagctgccg catcatgggc gttgacatgg acaaggacat
     1621 gccgcgcgcc aaatcattcc tggggctggt gccgcaggaa tttaatctca atatctttga
     1681 accgccgatg cagatacttg tgaaccaggc aggctactat ggtataacgc cgaaagtagc
     1741 gcgcaccagg gcagaaaaat acctcacgca gctgggcatc atggaaaagg cgcttacccc
     1801 gacgcgcatg ctgtcaggcg gtatgaaaag acggctgatg attgcacgcg cgctgatcca
     1861 cgaaccggca atactcattc tcgacgaacc taccgccggt gtggatattg aattgcgcaa
     1921 gctgatctgg aatttcctta ccgagctcaa tgcatcgggc acgactatca tactcaccac
     1981 gcactacctg gaagaagcag aaaacctctg caggaatatt gccatcatcg atcacgggca
     2041 gattatcgcc cacacggcga tgaagaagct gttgtcacgg ctaaaccagc agacatatgt
     2101 aatcgagctg gtgaaaccgc tggccaaggc tccacggctc agccacttcc agccggtgcg
     2161 caccgacaac accacgctcg aaatcacagt caagcgcaac cagcctatga atgcactctt
     2221 tgccgacttc agcgcaaaga agattggcat ccgcagtgtg cgcgaaaaaa ccaaccggct
     2281 cgaacagctg ttcctcgaac tgaccggcaa agcgggtaaa gtatgaaacg cgctgacctc
     2341 ataggctttt acacgatcct gcgcaaggaa tacatccgta tcgtgcgcat ctggtcgcag
     2401 acccttctgc cgccggttat gaacgttaca ctctacctgc tcatctttgg cagctttatc
     2461 ggcaaacgca tcggcacgat gggcggcgac agttacgcga gttttattgt tccggggctg
     2521 atactgatgg cggtgatcac caactcgtac ggcaatgtct cgagttcatt tttcagtgcc
     2581 aagttccaga agaatattga agagatactc gtctcgccga tacccgactg gctgatcgta
     2641 ctgggcttta cctttggcgg cattatccgt ggggccctgg tcgccctgtg cgtggcgctc
     2701 gtggtgctta ttgcccccgg ggtcgagctg agagtacaca acccgctgct ggtaatagtc
     2761 ctcttgctgc tgacagcagg cttcttttcg cttgccgggc tcatgaacgc catgtttgcg
     2821 aaaaaattcg atgacatcag ctttatcccg actttcgttt tgactccact gacttacctg
     2881 ggcggtatct tttatcccct ttctgccctg ccggaattct ggcagcgcgt ttcagccgtg
     2941 aacccggtgc tgcacatcgt caattcgctg cgctatgcgt tcctggggca tgcggagcta
     3001 ccatggtggt atgcggttgt tttcctgctg gcgggcaaca ccatactcta tatcgtctgc
     3061 ctgcgctacc tgaaaaaagg tctcggaatc cggaattaag attgactgcc cgcgcaattt
     3121 gcgcgatctc cggctatgga aatcctgcgc tggcaggctc tcaaccctct cgccaattac
     3181 cagtacatcg tctgcgacca tgaccgcaac gctgccttga tcgacccact cgacaccgac
     3241 caggtcgaat cgctcgccag ccggctttcg ctacgcatca aagctattct cgttacgcac
     3301 gagcatccgg accactcggg tgaggccggc cgtttgcaga atgccttcaa ggtgcccgta
     3361 tatagctccc acaattgcgc cggcgcagtc acctctgcac gcatagaagg tgtggccgat
     3421 aagacggagc ttcggctcag caaagaactt agcttcaggt ggcactatac cccaggccat
     3481 acaccgggcc atgccgtgat cgaggccgac ggcctgctct tcacagggga ttgcctgttc
     3541 cacgctggct gtgggcactg ccgcagcgct ggagcgaatg tcgaagatca ctacgcgtcg
     3601 ctgcacacgc ggctggccgc gctgacccct gacctgctgc ttttcccggg acattattat
     3661 gcccggcgta atctcgattt tgccctgcat gtagaacctg ataaccgccg ggcacgcgat
     3721 ctgcgacatc gcctggcgaa tgacggggac gaaatgaacc accagacaac cctgcgccag
     3781 gaacgcgaat acaacccgtt cctgcgtacc gaaagccggt cgctgcgccg gcgcgtaggt
     3841 gaactgagcg ggaaagacct gaccgaagcc agtggtttac aggttttcat cgagctgcgg
     3901 aaactgcgcg atacctggta atagtctgca atctttccgc aacatttgtt tacactgcat
     3961 ccggcccagc ccttctgacg tgtccgcaag gataattttt tcgaaggaaa actcgatgaa
     4021 aaaactgaca gcaatcgctc tgctggctgt agcaatgtct ttcgtagctt gctcatccac
     4081 agaagaaaaa aaagcagaag ttcctgctga agctccaaaa gctgaagtaa aagctcctgc
     4141 tcctgcagct gctcctgctc ctgcaaaagc acctgcaaaa aagaagaagt aatctcttct
     4201 tttaaagccc gcgcgatacg aaaagtgttg ccgcgggctt ttttatttgt atattggaca
     4261 aaagttcaat atgacgaacc tgacaacact gctgacggcg ctcgaaaaga ctgcccccca
     4321 ggccccactg caggatacta tccggcagct cagcggtagc ttcagtaacc tgcagattgt
     4381 attctcctct tcgctctcgc tcgaagacca ggcaattaca cacatcattg ccgaacacca
     4441 gctgcccgtg cgcatcttca cgctcgatac cgggcggcat tttccggaaa cgtatcgcac
     4501 actcgaggcc acgcgcgagc gctacggcag tgaaccagaa gtgtattttc ccgactttgc
     4561 ggccgttgag cgcctgatga agg
//
LOCUS       DAOQJF010000270         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11285_length_4563_cov_5.097604, whole genome shotgun sequence.
ACCESSION   DAOQJF010000270 DAOQJF010000000
VERSION     DAOQJF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11285_length_4563_cov_5.097604"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PLF85_12940"
     CDS             complement(<1..1047)
                     /locus_tag="PLF85_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HNE20625.1"
                     /translation="MPARDAWGSKLGIILAVTGSAVGLGNFLRFPGLAAKYGGGTFMI
                     PYFLAFLLLGLPIAYIEWSIARYGGTRGYHSAPGIFSVLWPRRISPYLGVLGLIVPVG
                     IYMYYVVIEAWCLYYAWGFLTGALNLGRDVNAYRDFFAAFTGSGADGSLFGGFSPALV
                     CIAVCYTLNFYFVYRGIAGIEKVSRIGMPLLLLTAVVVLVRVLTLGTPNPELPERSVI
                     NGLGHLWNPPQQAGLLESLMNAQLWMDAAGQIFFSLSVGFGVIINYASYLSKKDDILL
                     SATTAASGNEFAEVALGGLITVPAAFVFLGASGIGSTFGLGFVSLPTVFAHMPLGQIF
                     GFFWFLLLFIAAITS"
     gene            1129..2697
                     /locus_tag="PLF85_12945"
     CDS             1129..2697
                     /locus_tag="PLF85_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE20626.1"
                     /translation="MTRLAAWWDRYLNSGSHHFETATELKHNRVLNLLFSLNFVADAF
                     LVAAELTIFAVLLQRDFGTYIHYFWPFAIINFAHPVLIAVIIFLKNRLGTFKITYLST
                     VIYTAYCVTLSAFLGEQVGIHLILLVVIPIVFIMYDYGSWKEISVHSIIMSAGLIASL
                     VIYRVQPPLYPLPQDVAQVAGFLCYFAAIGLLFIYSIFNWKQVHLTEKLLADEKLQVE
                     GLLGETIPKLEIAEAKYRHLVDDSADLIFQMDEDGRILSMNKTCRFMLGFNPDEMVGS
                     TLYDFVTDAAEGDPELNRNIVRARVREFLDSKGIIHFRTTLRKKHLYEPLDVDITLQK
                     NLIQRTVEILGKVSRPQEDIAQRFLEKEKGRYIIGNNVTHAEVLSQRLTERLARYFPT
                     PVLNTIRICFREILINAIEHGNLGITFDEKTRAIESGDYMEYLLARQANPVYADRRVY
                     IDYLINERVLMFRVTDEGDGFDHRAFLGRAANDEYMAMLEHGRGIIMTKNVFDSLVFN
                     EKGNQVIMSKKIPQ"
     gene            complement(2748..3548)
                     /locus_tag="PLF85_12950"
     CDS             complement(2748..3548)
                     /locus_tag="PLF85_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HNE20627.1"
                     /translation="MNAVAWSGSKFVIAGDSGVVLTSSEGTSWASQTSGTAQALRAVL
                     WSGSQFVAVGANGTILTSANGTAWIPQTSGTTSILEAITWTGSQFVCAGYSGTILTSP
                     NATSWTSRTSGTTNDLYAIAWTGSQLVATGSNGTILTSADGITWVSRASGSVRLLSGI
                     AWSGSRLTVVGYDGSTFAPVILTSEDGISWSSVSAGSKAIADVVWSGTQFLAVGGTSG
                     SALIASSSTGLAWSGHNINLTEVLRGVTWSGSQYVAVGSNGSIYTATP"
     gene            complement(3747..4283)
                     /locus_tag="PLF85_12955"
     CDS             complement(3747..4283)
                     /locus_tag="PLF85_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE20628.1"
                     /translation="MRKITYHVATTLDGFIAHTDGTTEGFSFEGPHVTAYLEHLQEYD
                     TVIMGKGTYEAGYPFGLKPGEAAYPWMKNYVFSKSADFEIAHTDKLFVIRDNWRETID
                     VLRNQPGSDIYLCGGGKFAAALLAEGLVDRLRLKVSPMVFGSGVRLFEGNDLQCGFKL
                     DDITRYSSDTFLAEYSRK"
BASE COUNT         1127 a         1318 c         1189 g          929 t
ORIGIN      
        1 actcgtgatc gcagcaatga agagcagcaa aaaccagaag aaaccgaaga tctgcccgag
       61 cggcatgtgg gcaaaaaccg tcggcagcga aacaaagccg agaccaaagg tgctgccgat
      121 gcccgaggcc ccgagaaaga caaaagccgc gggtaccgta atcaaaccgc caagcgctac
      181 ctcggcgaac tcgttgcccg atgcggctgt cgtggccgag agcaggatgt cgtctttctt
      241 actcaggtaa ctcgcgtagt tgatgatgac accaaaaccg acagagagcg agaagaagat
      301 ctgcccggct gcgtccatcc agagctgcgc gttcatgagc gactcgagca gccccgcctg
      361 ctgcggcggg ttccagaggt ggccgagccc gttgatcacg ctacgctcgg gtagttcagg
      421 gtttggagtg cccagagtga gaacacgcac gagcactaca accgccgtca gaagtagcag
      481 cggcatgccg atacggctaa ccttctcaat acccgctatg ccgcggtaga caaagtagaa
      541 gttgagtgtg taacatacgg caatacagac cagtgcaggg gaaaatcccc cgaagagcga
      601 accgtcagcg ccgctgccgg taaatgccgc gaaaaaatcg cggtaggcat tcacatcgcg
      661 gcccaggttg agcgcccccg tgagaaatcc ccatgcataa tagaggcacc acgcttcgat
      721 gaccacgtag tacatgtaga tgcccaccgg aacaatgagc cccagcacac cgaggtaagg
      781 tgaaatgcgg cgcggccaga gcacgctgaa gatgccggga gcgctgtggt agccgcgagt
      841 gccgccgtag cgggcaattg accactcgat gtacgcaatc ggcaggccca gcagcagaaa
      901 ggcaaggaaa tacgggatca tgaaagtgcc gccgccgtac ttggcagcaa gcccgggaaa
      961 acgcaaaaaa ttgccgaggc caaccgcaga cccggtgacc gccagaataa taccgagctt
     1021 acttccccat gcgtcgcgtg ccggcattct ggcttgctcg aaacttcgga cgaaccggtc
     1081 aagctaaacg tggtttacga cgtaatcgtg tgcgtaatgc caaccaccat gacgcgactc
     1141 gctgcatggt gggatcgata cctgaattcg ggcagtcacc atttcgagac tgcgaccgaa
     1201 ctcaaacaca accgcgtcct caacctgctg ttctcgctga actttgtcgc cgatgcattt
     1261 ctcgtcgcgg cggagcttac catcttcgcg gtactgctgc agcgcgattt cggtacctac
     1321 atccactact tctggccgtt cgcgatcatc aactttgccc accccgtact gattgcggtc
     1381 atcattttcc tgaagaaccg cctcggcaca ttcaaaatta cgtacctcag caccgtcatc
     1441 tacacggcct actgcgtcac gctgagcgcc tttctcggtg aacaggtcgg cattcacctc
     1501 attttgctcg tcgttattcc gattgtcttc atcatgtacg actacggcag ctggaaagag
     1561 atcagcgtgc actccatcat catgtcggcg ggtcttatcg ccagtctcgt gatctaccgg
     1621 gtgcagccac cgctctaccc cctgccgcag gatgtcgcgc aggtggcggg ttttctctgc
     1681 tactttgccg cgattggtct gctctttatc tactctatct tcaactggaa gcaggtacac
     1741 ctgacagaga agctgctcgc cgatgaaaag ctgcaggtcg aaggcctttt gggcgaaaca
     1801 atacccaagc tcgaaatcgc cgaggcgaaa taccgccacc tggtcgacga ttcggccgac
     1861 ctgatcttcc agatggacga agacggcagg attctcagca tgaataagac ctgccgcttc
     1921 atgctcggtt tcaaccccga cgaaatggtt ggcagtactc tgtacgattt cgtcaccgac
     1981 gccgccgagg gcgaccccga gctcaaccgg aacatcgtgc gcgcgcgcgt ccgcgaattt
     2041 ttggacagca agggcatcat tcacttcagg accacgctgc gcaagaagca cctctacgaa
     2101 ccgctcgatg tcgatatcac gctgcagaag aacctgattc agcgcacggt tgaaatactc
     2161 ggcaaggtat cgcgtccgca ggaagacatc gcgcagcgtt ttctcgagaa agaaaagggc
     2221 cgctacatca tcggcaacaa cgtgacccac gccgaggtct taagccaacg cctgaccgaa
     2281 cgccttgcgc gctactttcc caccccggta ctcaacacga tacgtatctg ctttcgcgag
     2341 attctcatca acgcgattga gcacggcaac ctgggcatca ctttcgacga aaaaacccgc
     2401 gcgatcgaaa gcggcgacta catggaatac ctgcttgcac ggcaggccaa ccctgtctat
     2461 gccgaccggc gcgtctacat cgactacctg atcaacgagc gcgtgctgat gttccgcgtc
     2521 accgacgagg gcgatggctt tgaccaccgc gcctttctcg gccgcgcggc caacgacgag
     2581 tacatggcca tgctagagca cggccgcggc atcatcatga caaagaatgt attcgattca
     2641 ctggtcttca acgaaaaggg caaccaggta atcatgtcga agaagattcc gcaatagcag
     2701 tgcgggcctg tggctcagct cctgcttata atcgagatac cccgcgccta cggtgtggca
     2761 gtatagatag agccattact acctacggcg acgtactgcg aaccggacca ggtgacgccc
     2821 cgcagtactt cggtcaggtt gatgttatgc cctgaccagg ccagtccggt cgagctactc
     2881 gcaatcaaag cagacccgga tgtaccgccg acggcgagaa actgcgtgcc cgaccaaacg
     2941 acatcggcaa tagctttgct gccggctgaa acagaggacc atgaaatacc atcctccgaa
     3001 gtcagaataa caggagcgaa agttgaacca tcatagccca caaccgtcag cctgctgcct
     3061 gaccaggcaa tacccgaaag cagcctgact gaaccggatg cacgcgagac ccaggtgatg
     3121 ccgtcagccg aagtcagtat cgtgccattg ctgcccgttg caacgagttg gctgcccgtc
     3181 catgcgattg catagaggtc gttggttgtg ccggaagttc tgcttgtcca gctcgtggcg
     3241 ttcggcgaag tcaaaattgt gccactgtaa ccggcacaga caaactggct gcctgtccag
     3301 gtgatggcct ccagtatgct ggttgtgccg gaagtttgag ggatccacgc cgtgccgttt
     3361 gcagatgtca gtatagtgcc attggctcca acggcgacaa attggctgcc cgaccaaagg
     3421 actgctcgca gcgcctgggc cgtgccagag gtttgcgatg cccaggatgt cccctcggaa
     3481 gatgtgagca caacaccgga atcgcctgca ataacaaact tcgagcctga ccatgctacc
     3541 gcattcaagg cactggaaac acctgagctc ctgggagtcc aattgagcgt gttgtcaatt
     3601 ggggaggagg aattagagca ttgccagcct gcaaacagaa ccagcacaaa tatgaatggt
     3661 aggctgcgtt tcatggacag tatcaccgga tcgcaatgac atcgaatcgt aaagtatttc
     3721 ctgccccggg tatccggggc aggctgctat ttcctcgaat attctgccag gaaggtatca
     3781 ctcgaatacc gggtaatatc atccagtttg aagccgcatt gaaggtcatt accttcaaac
     3841 agcctgacac ctgaaccgaa aaccatcggc gataccttca ggcgcaaccg gtcgaccaac
     3901 ccctctgcta aaagcgcagc agcaaatttg ccaccgccac acaggtagat gtcagaccca
     3961 ggttggttcc tcaggacatc gatggtttcg cgccagttat cccttataac aaacagctta
     4021 tctgtgtgcg ctatttcaaa atctgcagat ttcgaaaaaa catagttctt catccacgga
     4081 tacgccgctt caccgggctt caggccaaat ggataccctg cttcgtaagt tcctttgccc
     4141 atgatcacgg tgtcatattc ctgcaggtgc tccaggtagg ccgttacgtg cggaccttca
     4201 aagctgaagc cctcggttgt cccgtcggtg tgtgcaataa agccatctaa tgtcgtggca
     4261 acatggtaag tgatctttct catatagtac tcccttaaaa aatgattttt gtaaagaaat
     4321 ataccaccac acaaatctca gctttttcgg attgctggtg agtggcgcgg tttagagtac
     4381 catgatggtt tatcggagct tcttaggcag ttcgataccg ctggaatctt aataaaatac
     4441 tcggcagatg cccagaaaaa acatcactag cggaaaaatg tttagcagac cagattatga
     4501 ccatgccttt tagcagtctg cgccgtcgtt catcgttgcg ctataaaacc ggcaagacac
     4561 ctg
//
LOCUS       DAOQJF010000271         4507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11511_length_4507_cov_4.751572, whole genome shotgun sequence.
ACCESSION   DAOQJF010000271 DAOQJF010000000
VERSION     DAOQJF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11511_length_4507_cov_4.751572"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1362)
                     /locus_tag="PLF85_12960"
     CDS             complement(22..1362)
                     /locus_tag="PLF85_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNE20629.1"
                     /translation="MMTIFAERVKRLQQHMQPNSLLFMPAASGRIRNRDTQYRYRAHS
                     DTLYLTGVNEEELSLFVTGDALHICARTSDPERERWTGKVRGHEFFLERFADAPFSVT
                     VSAANDWQKKLAELARGRQVLYYDFGVDAEGDTRVLAQLAEMAQYARKGIYAPRTITR
                     AYDVLAPLRLIKDNHDIAVMRRAAAISAAAHNAAQDYIVSARGEVSEYAVKALIEHEF
                     MRQGADQLAYPSIVAAGANATVLHYEGTADRAQPGDFILIDAGAELEGYASDITRTTP
                     VGGFSRASAVRRDLYDVVLAAQKAAIAHSRPGNTIEAVHQKAVDVLCDGLLRLGLFER
                     VPDRSAGNEDRNRLVSLKSREQVAEHDYHTYFYMHRTSHFLGLDVHDVGDYHIAGKSR
                     MLEPGMVITVEPGLYFPPEYDFLAPEVRGIGIRIEDDVLITSDGNEILTVSCRS"
     gene            1481..2584
                     /locus_tag="PLF85_12965"
     CDS             1481..2584
                     /locus_tag="PLF85_12965"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNE20630.1"
                     /translation="MDILDRYIFRNFLKAFAGTLLLLVGVSVIVKILDSLKDFTDNQH
                     GVGLLLEYYLVVTPSFVTYIVPPALMFATAFTISNFNRNFEITVMLAAGRSFRRILRP
                     ILIFTALFSVAFFAFNEYVAFPSAYRAVDISYQLRNKGEDARLRKYDKSGDMTLRFGN
                     RYYTIGHGAWYRRELVGFHLLEISQQGSIARIVEADSATVENHETRQWLMKNVRITRF
                     NEQGLYTGTDSHEVLAVELSENLRAFQNFYVEMDSEERSLFDAFRIYQKRKQSGGSYE
                     VFLTEVFWHIGYPLVCLFIVFIGGMLGGKLKKGGVPVSIAVSMLFTLGYFFFMYFGTA
                     FGDSGTLPPFIAGNLANLVAGSLSTWIYFRLDY"
     gene            2616..4067
                     /locus_tag="PLF85_12970"
     CDS             2616..4067
                     /locus_tag="PLF85_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20631.1"
                     /translation="MKAFYTRHPLLVILAAAFLLRTAAAFYNYTPTAEDDYANIIEPA
                     LKHFQTGAAITTELYRLPALPKVFFAFLLPLKAAGVADTTLLVSWGLFCLGLISLAGV
                     WAFYQLARHVVPGWENTAGWLYAGHFLLPFFSTRAFQESLTLTTVPLALYFLWYPDRP
                     VNRLFFAGLFLGLTVIFRFQAAILAATVFFYLSYEFLRRQTSARQLLAFASGGLVALV
                     LLVLLDIAEGRAALSTPFEYIRINFMGNVVSQNYGAAPWHTYLTLLTVVFIPPFSFVL
                     LQPLLRAMRSDLLSTICLGVFLVSHSMIGNKLERFMLPIVPFFFLLTLRGLRESENSR
                     IIKVAWRGFIVLNLLVLVPITLSRSQLNMIHAAQHLASSTRPLLLYRIDLWKQAYMTF
                     AKPEPANFADVTSLAAGFRALAAPEADLLSLGKISPEAEKEINGAGYQCRQERTFEPS
                     WQERIVIYLNPRFNTRRDTSVLYRCVRQTSVPR"
     gene            complement(4042..>4507)
                     /locus_tag="PLF85_12975"
     CDS             complement(4042..>4507)
                     /locus_tag="PLF85_12975"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE20632.1"
                     /translation="SKLTASIALAYLVALYDLKFGRFVPLRFVKYALVGLSGIFINAL
                     AFWLARNSLELRDSRALVLAILVSIASNFFLNNFFTFSDRRLAGFRNLLWGYLKYNFI
                     CLGGAFINYAITMNLRNDFALNIDAANLVGVGVATFWNYFINLNLTWGQKSA"
BASE COUNT          950 a         1310 c         1187 g         1060 t
ORIGIN      
        1 acaacgcgca acagcttcac ttcatgacct gcacgatacc gtcaatattt cgttaccgtc
       61 gctggtaatc aaaacatcat cttcaatgcg aatgccgata ccacgcacct cgggagcaag
      121 aaaatcatac tcagggggga aataaagtcc cggttcaacc gtgatgacca ttccgggttc
      181 gagcatcctg ctttttcctg ctatatgata atcgccgaca tcgtgtacgt cgaggccaag
      241 aaagtgcgaa gtcctgtgca tgtaaaaata tgtatggtaa tcgtgctcgg caacctgctc
      301 cctgcttttg agggagacca ggcggttacg gtcctcgtta cccgcactgc ggtcgggaac
      361 gcgctcgaag agcccgagac gcagtaaccc gtcgcagagg acatcgacgg ctttctggtg
      421 cacggcctca atggtattac cggggcggga atgtgcaata gccgccttct gcgccgcgag
      481 cacaacgtcg tagaggtcgc gccgcactgc tgacgcgcgg gaaaaacctc cgaccggcgt
      541 cgtgcgggtg atgtcggagg cgtaaccttc aagctcagcg ccggcatcga tcaatataaa
      601 atctcccggt tgtgcgcggt ccgctgtgcc ctcgtagtgc agaaccgtcg cgttcgcacc
      661 ggcggcgaca atcgacgggt aagcgagctg gtctgccccc tgccgcatga actcgtgttc
      721 aatgagcgcc ttcacggcgt actcactcac ctcacccctg gctgaaacga tatagtcctg
      781 cgcggcgtta tgcgccgcgg cagatatggc ggccgcacgg cgcatgaccg caatgtcatg
      841 gttatctttg atgaggcgca gcggcgccag tacatcgtat gcacgcgtaa tcgttcgcgg
      901 ggcatagata cctttgcggg catactgcgc catttcagcc aactgcgcca gcacgcgcgt
      961 atcgccttcc gcatcgacgc caaaatcgta ataaagcacc tggcggccac gcgccagttc
     1021 tgcgagtttt ttctgccagt catttgccgc gctgaccgtg acgctgaaag gagcgtcggc
     1081 aaaacgctcc aggaaaaact catgcccgcg caccttacca gtccagcgct cacgttcggg
     1141 atcgctggtg cgcgcgcaga tgtgcagcgc gtctccggtg acgaagagcg aaagctcctc
     1201 ttcgtttacg ccggtcaggt acagggtatc ggagtgcgcc cggtaacggt actgagtgtc
     1261 gcggttgcgt atacgcccgc tggcagcagg cataaaaagc agcgaattcg gctgcatatg
     1321 ctgctgcagc ctctttaccc gttcggcgaa tattgtcatc attctcgtgc gcagggtcag
     1381 cgggggagct gctgcgtcat ctcttttgga ctggtgcgaa tcgacatgac agctccggcg
     1441 gaaactggga ttcggcaacc tgactctcag ctgagcgcgc atggacattc tcgaccgcta
     1501 cattttccgc aatttcctga aagcctttgc cggtaccctg ttgctgctgg tcggcgtctc
     1561 ggtcatcgtg aagattcttg attcgctcaa ggatttcacg gataaccagc acggggttgg
     1621 gctcctgctc gaatactacc tggtagtaac accctcattc gttacgtaca tcgtgccccc
     1681 agctctcatg tttgcgactg cgttcacgat ttcgaatttt aaccggaatt tcgagattac
     1741 cgtgatgctc gccgctgggc gttcatttcg gcgcatcctg cggccaatac tcattttcac
     1801 tgccctgttc tcggtggcct tctttgcctt caacgaatat gtcgcctttc cttctgccta
     1861 cagggccgtc gacatcagct accagctgcg caacaaggga gaagatgcgc gactgcgaaa
     1921 atacgataag agcggcgaca tgacgctccg gttcggcaac cgctactaca caatcggcca
     1981 cggagcctgg taccgccgtg aacttgttgg ctttcacctg ctcgaaattt cgcaacaggg
     2041 cagtattgca cgtatcgtcg aagcagacag tgcgaccgtc gaaaaccatg aaacacggca
     2101 atggttaatg aagaatgtgc gcatcacgcg cttcaatgag cagggtctgt acacgggcac
     2161 cgattcacac gaggtgctgg cagtcgaact ctctgagaac ctccgcgctt tccagaactt
     2221 ctatgttgag atggactctg aagaacgttc gcttttcgac gctttccgca tctaccaaaa
     2281 acggaaacag tcaggcggct cgtacgaggt attcctgacc gaggtattct ggcatatagg
     2341 ttacccgctg gtttgcctct tcatcgtttt cattggtggt atgctcggcg gaaagctgaa
     2401 gaaaggaggc gtaccggtca gcatcgcggt atcgatgctc ttcaccctcg gctatttctt
     2461 cttcatgtat tttggcaccg cctttggcga cagtggtacc ctaccccctt tcatcgccgg
     2521 caacctcgca aacctggtag ccggcagcct ttccacctgg atctatttcc gactcgacta
     2581 ctaatcctat aacactgcat tacccggaga agcttatgaa agccttttac acacgccatc
     2641 ctctgctcgt catactggcg gccgcttttt tgctcagaac tgctgcagct ttttacaact
     2701 acactcccac tgcagaagat gactatgcca acatcatcga accggcgctg aagcacttcc
     2761 agaccggcgc agcgatcact accgaactct accggttgcc cgcattgccc aaggtgtttt
     2821 ttgccttcct gttgccactc aaggcggccg gtgtcgcaga caccacctta ctggtttcgt
     2881 gggggctctt ttgccttggt ctcatttctt tagcgggagt ttgggcgttc taccagcttg
     2941 cccgccacgt tgttcctggc tgggaaaaca ccgctggctg gctttacgcc gggcatttcc
     3001 tgctgccatt tttttccaca cgggcctttc aggaaagcct gacgctcaca accgtgccgc
     3061 tggcgctgta tttcctctgg tatcccgatc ggccggtaaa ccgcctgttt ttcgcggggc
     3121 tttttctggg gctaaccgtt atctttcgct tccaggcggc aatactcgct gcgactgtct
     3181 tcttttacct ttcgtacgaa tttctgcggc gacagacgag cgcacggcag ctgctggcat
     3241 ttgccagtgg cggtctcgtt gcgctggtat tgctggtact gctcgatatc gccgagggcc
     3301 gcgccgcgct ttcgaccccg ttcgaataca tccgcattaa cttcatgggt aatgtggtca
     3361 gccagaatta cggtgccgcg ccctggcaca cctacctgac gctgctgaca gtggtgttta
     3421 ttccgccgtt ttcatttgtg ctgctgcagc ctctcttgcg ggccatgcgc agcgatctgc
     3481 tcagtaccat ttgcctcggc gtgttcctgg tttcgcacag catgatcggc aacaagctcg
     3541 agcgcttcat gctgcccata gtaccttttt tcttcctgct gacactgagg gggctgcgcg
     3601 agtcggagaa ctcccgcata ataaaggttg catggcgtgg gtttatcgtg ctcaacctgc
     3661 tggttctcgt gccgattacg ctcagccgca gccagctcaa catgatacac gccgcgcaac
     3721 accttgccag ttctacgcga ccgcttctgc tctaccgcat tgacctgtgg aagcaggctt
     3781 acatgacttt cgccaagcct gaacctgcca acttcgctga tgttacttcg ctggccgcgg
     3841 gttttcgagc gctggctgcg cccgaggcag acctgttgtc gctcgggaaa atttcccccg
     3901 aagctgaaaa ggaaatcaat ggggcaggct accaatgccg ccaggagagg acatttgaac
     3961 cttcatggca ggaaagaatc gttatttacc tgaacccgcg cttcaataca aggcgcgata
     4021 cctcggtact ctaccgttgc gtcaggcaga cttctgtccc caggtaagat tcaggttgat
     4081 gaaatagttc cagaatgttg cgacgccgac ccccacgagg tttgccgcgt caatgttgag
     4141 agcaaaatca ttcctcaggt tcatggtaat cgcgtaatta atgaacgctc ccccgaggca
     4201 gataaaatta tatttcaggt aaccccacaa caggttacga aaacctgcga gtcggcgatc
     4261 gctgaaggta aaaaagttgt tcagaaagaa gttggaggca atactgacca aaatggccag
     4321 gacaagagcg cgtgaatcac gcagctcaag cgagttccgc gccagccaga aggccagcgc
     4381 gttgatgaag atgcccgaca ggccaacgag ggcatacttt acgaaacgca aaggcacaaa
     4441 cctgccgaac ttgagatcgt agagcgcaac gaggtaagcc agagcaatag acgccgtgag
     4501 cttgctt
//
LOCUS       DAOQJF010000272         4503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11528_length_4503_cov_4.530126, whole genome shotgun sequence.
ACCESSION   DAOQJF010000272 DAOQJF010000000
VERSION     DAOQJF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11528_length_4503_cov_4.530126"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            421..660
                     /locus_tag="PLF85_12980"
                     /pseudo
     CDS             421..660
                     /locus_tag="PLF85_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            835..1176
                     /locus_tag="PLF85_12985"
     CDS             835..1176
                     /locus_tag="PLF85_12985"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HNE20633.1"
                     /translation="MKISSLFLLLPILYTSLLFAKPKPRHLPHYNDVRYTCANGRVIE
                     ARFILDEPGRVKLRLSDGRKLELPQAFAASGARYANAKETFVFWTKGDDAFIVEEEIL
                     TYRDCTSQKGP"
     gene            1257..2495
                     /locus_tag="PLF85_12990"
     CDS             1257..2495
                     /locus_tag="PLF85_12990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNE20634.1"
                     /translation="MAAAASAIQQTPPTRFRDPAENRDLFLKLADALFIRRKVSHEEI
                     ARYGSYLWAGDALADRLAKKFDEIGMQRAMPMLNTALHGNLPADAPDELVEFIAETKK
                     DPAWLDRALLEEGAKLSQRSGPFGEYVLSCVSLMGGYRSAAANKPIAFTGQLEYMAPR
                     RLAETAKFGYDVTRTGAMAQGAPGFNAALKVRMMHAHVRLMLDRSPKWRHGDWGLPIN
                     QADMIATNILFSSVYLTGLRILGLQISDRESQGVMHLWRYIGWLMGIDEALLPENEEQ
                     GMRMAWLAGVTQPGADADSRLLGQALLNVPMERAESPVEKALAWLEMQLRSGFTRIVL
                     GDRAGDDLGLPDSPLKYTVVFTTPAVFALETLRRVLPGATDVAIRLGDAWQRRMVEKP
                     LGGKMPAFVPPQQLRHEAAY"
     gene            complement(2492..4213)
                     /locus_tag="PLF85_12995"
     CDS             complement(2492..4213)
                     /locus_tag="PLF85_12995"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNE20635.1"
                     /translation="MRSYAEALAVAESWTRAPFDNDTITSVRAILSLPDAEREKAILD
                     QFGYDLSFGTAGMRALMDVGTARMNQYTIARVSAAVAQVLKSQVKDKTPKVVIGFDAR
                     RNSEKFAKIAAQVYLAAGIEVHRFNRIVPTPVVPFFVRRLGADCGVVITSSHNTAPYN
                     GYKVYAANGAQILSPFDTDVMAAMAQLPYALPTAAAQGGKLVDLSDEILRDYVSWVVS
                     EVDFAKDRSQKIVFTPLHGAAGALMNAVFYKSGFKNFLPVPQQYEPHPGFPTVKAPNP
                     ENKDSLVMALEKAEEDKADLILATDGDGDRIGIAYRNSSGKFETLTGNQIGTVLLYYL
                     LCKLREQKKLRPNLFALCSVVSTPLTRTICEKFGITFHETLTGFKNMGNKAHEIQLQD
                     PNARMVLAYEEAFGVTIGDSRDKDGIISNLLAAHIAADHGGFAHWLKAMYDECGYAAE
                     DAVEKEFPGAAGIDAMTNLIEQIRQAPPKEFLGHPVALARDFKSLKEIRDGKEFAITH
                     VPAQNLLYFTNANGDWVAIRPSGTEPKVKAYLGVREQSAFSAKTVAHAHSRLAELKKF
                     AASLLGG"
     gene            4261..>4503
                     /locus_tag="PLF85_13000"
     CDS             4261..>4503
                     /locus_tag="PLF85_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNE20636.1"
                     /translation="MRTPLIAGNWKMNLNMAEAEALAKAVGAAAAQYSDREVLVYPTF
                     LQFTAAKAALAGSKVKLGIQNCSAEKDGAFTGEISAS"
BASE COUNT          900 a         1253 c         1337 g         1013 t
ORIGIN      
        1 gttcggtcgc ggcccgctta tctgccgcag tcttgtcgat tgcagctttg cgctctgcag
       61 ccagctcggc agcggtcttt ttctttgcct gtgctgtaag cgccagtggg ctcatggcca
      121 gtgccagaat catgatcatc gtttttttca tgagttgtct cctaatagta taatctgccc
      181 aatgggcgga ggtatctatc tttccgggtg ataggccgaa tagcagatca ggtttattgc
      241 tggacagcct gttgccctgg ggtcagcttc ggccgaaccg ctgaattttg aatgatcacg
      301 atttctggtt gtctttgaac gaagtttgcc tgtatttccg ctcatttgta atcgggttta
      361 aaaaagctgc agcaacctgc cttgcaggcc agagcggcga gttagaaaag aggattttga
      421 atgaacattt atgtaggtaa ccttgcgtac acagtgaccg aacagcagct ggcgcaggct
      481 ttttctgaat ttggaacagt gagctctgcg aaactgatta tggaccgtga caccggtcgt
      541 gcgaaaggct ttgcctttgt cgaaatggct gataagtcag aaggcatcaa ggcgattgcg
      601 ggcatgaacg gccgcgaact gaacaaccgt ccgatcgttg tcaacgaagc acgcccgcgt
      661 gaagaacgtg gcggtttcgg tggtggcggc ggcggccgtg gtggcaaccg cggcggcggc
      721 ggtggtggtc gcggtggtaa ccgcggcggc tacaacgatc gctggtaaaa aaaatgggag
      781 cttaacggct ccaaaaacat tttcccggcc atgctgcaca cgcagtgtgg ccgggtgaaa
      841 atttcttctc tctttttgct tctcccaatt ctctatactt ctctcctttt tgcgaaacca
      901 aaaccgcggc atttaccgca ctataacgat gtgcgctaca cctgtgccaa tggcagggtt
      961 attgaagcac gcttcatact cgacgaaccg ggcagggtta agctgcgcct gagcgacggc
     1021 cgcaagctgg aactgccgca ggcatttgcc gcatcgggcg cacgctacgc caacgcaaaa
     1081 gaaacttttg tcttctggac gaagggcgac gacgcattta tcgtcgaaga agaaatactg
     1141 acctaccgcg attgcactag ccagaaaggc ccgtgaagcg gtgtgtccga aatggcagct
     1201 tttaccgggc ctgctaatct cacttgacgc tccgcttacc gccaccgcgg taaataatgg
     1261 cagcagccgc ttctgcaatt cagcaaacac caccgacgcg ttttcgcgac ccggcagaaa
     1321 atcgtgacct gtttctcaaa ctcgccgatg cgctgttcat tcggcgtaag gtcagccacg
     1381 aagaaattgc gcgctacggc agttacctct gggccggcga tgcactggct gatcggctgg
     1441 caaaaaaatt cgatgagatc ggcatgcaac gggcaatgcc aatgctcaat accgcgctgc
     1501 acggtaatct gccggccgat gcgccagacg aactagtgga gttcattgct gaaacgaaaa
     1561 aagatcctgc gtggctcgat cgtgcgctgc tcgaagaggg agcaaaactc tctcagcgct
     1621 caggcccgtt cggtgaatac gtactttcgt gcgtcagtct catgggtggc tatcgctccg
     1681 ccgccgccaa caagccgata gccttcacgg gacagctcga gtacatggcg ccgcgccgcc
     1741 tcgccgagac tgccaagttt ggttatgatg tcacgcgcac gggggcgatg gcgcaggggg
     1801 ccccggggtt taatgctgca ctcaaggtcc gcatgatgca tgcgcatgtg cggctgatgc
     1861 tcgaccgttc gccgaaatgg cgccacggcg actggggttt gcccatcaac caggcggaca
     1921 tgattgccac caatattctt ttttcatcgg tttaccttac cgggctgcgc atactcggcc
     1981 tgcagatttc tgaccgcgag tcgcaggggg tgatgcacct ctggcgctac attggctggt
     2041 tgatgggcat tgatgaagcg ctgctgcccg aaaatgaaga gcagggcatg cgcatggcat
     2101 ggcttgccgg tgtcacgcaa ccgggcgccg atgcagattc gcgtctgctt gggcaggcgc
     2161 tgctaaacgt gcccatggaa cgcgccgaat cgccggtgga aaaagctctc gcgtggcttg
     2221 agatgcagct gcggtcaggt tttacgcgga tcgtgctcgg cgaccgcgcg ggcgatgatt
     2281 tgggtttacc cgattcgccg ttgaagtaca cagtggtctt caccacaccc gccgtattcg
     2341 cgctcgagac cttgcggcgc gtgttgcccg gtgcaaccga tgtggccatt aggctgggcg
     2401 acgcctggca gcggcgcatg gttgaaaagc ccttaggcgg caaaatgccc gcgtttgtgc
     2461 ccccgcagca actgcgacac gaggctgctt actagcctcc caaaagcgaa gcggcgaact
     2521 ttttgagttc agccagcctg ctgtgcgcgt gcgccacggt cttggccgaa aatgcggact
     2581 gctcccgcac accgaggtat gcctttactt tgggttcagt gcccgaaggc ctgatggcaa
     2641 cccaatcgcc gttggcgttg gtgaagtaga gcaggttctg tgccggcacg tgggtgatcg
     2701 cgaattcttt gccgtcacga atttctttga gtgacttaaa atcgcgagcc aaagcaaccg
     2761 ggtgacccag gaattctttc ggtggcgcct ggcgtatctg ctcgatcagg ttcgtcatcg
     2821 cgtcgatgcc cgcagccccg ggaaactcct tctcgaccgc atcttctgca gcgtagccgc
     2881 attcgtcgta catcgccttg agccagtgcg cgaagccgcc gtgatccgct gctatgtgcg
     2941 cggcgagcag gttgctgatg ataccgtcct tgtcgcgtga atcgccgatg gtcacgccga
     3001 acgcctcttc gtacgcgagc accatgcgcg cattggggtc ttgcagctgg atctcgtgtg
     3061 ccttgttgcc catgttcttg aaaccggtca atgtttcgtg aaaggtgatg ccaaacttct
     3121 cgcagatggt gcgggtgagc ggcgtgctca cgacgctgca gagcgcaaag aggttggggc
     3181 ggagcttctt ctgctcgcgc agcttgcaca gcaggtagta gagcagcacc gtgccgatct
     3241 ggttacccgt cagggtttcg aattttccag aactgttacg gtatgcaatg ccgatgcggt
     3301 caccgtcacc gtcggtggcc agtatcaggt cggctttgtc ctcttcggct ttctcgagcg
     3361 ccatgaccag gctgtccttg ttttcggggt ttggcgcctt gaccgtggga aaacctggat
     3421 gcggttcgta ctgctgcggt accggcagaa agtttttaaa tcccgatttg tagaaaacgg
     3481 cattcatcag cgcaccggcc gccccgtgca gtggcgtgaa gacaatcttc tgcgaccggt
     3541 ctttggcgaa gtcgacttcg ctcaccaccc agctcacgta atcgcgcaga atttcgtcag
     3601 acaggtcgac caacttgcca ccttgcgcgg ctgcagtcgg cagtgcatac ggcagctgcg
     3661 ccatggcggc catgacgtcg gtgtcgaacg gtgaaagaat ctgcgcgccg ttcgccgcat
     3721 acaccttgta cccgttgtag ggagcggtat tgtggctgga agtgatcacg acgccgcaat
     3781 cggcgcccag cctgcgcacg aagaagggta caacgggcgt cggtacaata cggttgaagc
     3841 ggtgcacttc gatacccgcc gcgaggtaca cctgcgccgc gatcttcgcg aatttttctg
     3901 agttgcgacg cgcgtcgaag ccgataacga ccttcggagt tttgtctttc acctgcgact
     3961 ttagcacctg cgcgactgcc gcactgacgc gcgcgatcgt gtattggttc atgcgtgcgg
     4021 tgccgacgtc catgagagcc cgcatgcctg ccgtgccgaa gctgaggtca tagccgaact
     4081 ggtcgagaat cgccttctcg cgttctgcgt cgggcagcga cagtatcgcc cggacgctgg
     4141 taatagtgtc gttgtcaaaa ggtgccctgg tccacgattc ggcgaccgca agggcttctg
     4201 cgtagctgcg catggagaat tttttcgttt acgtgcgtag cacgtaaacg aattaactct
     4261 atgcgaactc cactcatagc tggtaactgg aagatgaacc tcaacatggc cgaggctgaa
     4321 gccctggcaa aagccgtcgg cgctgcggcg gcgcagtaca gtgaccgcga ggtgctggtg
     4381 tacccaacgt tcctgcagtt cactgccgct aaggccgcgc tcgcgggctc gaaagtgaag
     4441 ctcggtatac agaattgctc ggccgaaaaa gacggcgcgt tcacaggtga aatttcggcg
     4501 tcg
//
LOCUS       DAOQJF010000273         4499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11541_length_4499_cov_5.295680, whole genome shotgun sequence.
ACCESSION   DAOQJF010000273 DAOQJF010000000
VERSION     DAOQJF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11541_length_4499_cov_5.295680"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLF85_13005"
     CDS             complement(<1..788)
                     /locus_tag="PLF85_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNE20637.1"
                     /translation="MGKPTGFKEYPRATQKSDPVPDRVKHYKEFEHTFETDTAKVQGA
                     RCMDCGIPFCHGDTGCPVDNLIPEFNDLVYRGRYEDALENLHTTNNFPEFTGRLCPAP
                     CEGACTLGINEPAVAIKGIERFIIDTGFKNGWVKPQPPTHKTGKKVAVVGSGPAGMAA
                     SQQLVRMGHSVTLFEKNEKIGGLIRFGIPDFKFEKWQIDRRAEQMVAEGLVIKTGVTV
                     GKDIPAEQLVKDFDAVILAGGAEAPRDLPIPGRELKGVHFAMEFL"
     gene            complement(781..>4499)
                     /gene="gltB"
                     /locus_tag="PLF85_13010"
     CDS             complement(781..>4499)
                     /gene="gltB"
                     /locus_tag="PLF85_13010"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNE20638.1"
                     /translation="IVMEGQSDSATFDTVLELLVMSGRSLPHAIMMMIPEAWSKNDLM
                     SEERRAFYEYHATMMEPWDGPAAVAFTDGNVIGATLDRNGLRPARYIKTKDDIVIMAS
                     EMGVLEVPPEEIVLSGKLEPGRMFLIDLSEHRIISDDEVKDKICKQQPYGKWVKENMI
                     KFADLPAPKDGHTPEHKTMLERQRIFGYTKEDLLTVMKPMVVTGQEAVGSMGTDAALA
                     VLSEKPQLLFRYFKQLFAQVTNPPVDAIREELVMELTTYIGPEQNLLDETPLHAHRIE
                     LPHPILENNQLQQLIEIAKGQFSAKVFSLHFNPAKKNDLRTQLDKICASVCDAVRQGA
                     NIVILSDRGVSREVAPIPSLIAVAAVHHALIRAGLRTRTGIVLESGEPREVHHFALLL
                     GYGANAINPYLAYETLHDLYKIGFMPELKEETYIEKNYRKAIGKGLFKIFSKMGISTL
                     QSYCGAQIFEAVGLDTEVVEQYFTGTPSRVEGVSLEMLEEEARRRHEEAFGLLKEGIL
                     PSGGLYHYRVDGEAHLWNPTTVAKLQISTRNSDYKTFKEYTAAIDDQSKRRVTLRSLF
                     EFDYAANPIPLEEVEPAKEIVKRFATGAMSFGSISWEAHTNLAIAMNRIGGKSNTGEG
                     GEGAERFVTLPNGDSLRSAIKQVASGRFGVTANYLINADELQIKMAQGAKPGEGGQLP
                     GFKVDRIIGKVRHSTPGVTLISPPPHHDIYSIEDLKQLIFDLKNINPRARISVKLVSE
                     SGVGTVAAGVAKAHADHILISGHDGGTGASPISSIQYAGTPWELGLAETHQVLVVNGL
                     RDRVYVQADGQMKTGRDVVIAALLGAEEFGFATAPLVTLGCIMMRKCHLNTCPVGVAT
                     QDPELRKKFNGKPEHVVNFMFYIAEEVREYMAKLGFRKLTDMVGRTEKLIPSRPKDHW
                     KARGLDLSKLLTMQKPVYKTDLYRTKAQNHEIEKQIDNRIIEMAKPALDRKEPVNIQL
                     PVTNVDRTVGAMLSGEVAKRYGDEGLPHDTIQIQMTGTAGQSFGCFLAKGVTLKLSGQ
                     ANDYCGKGLSGGRLVVRLPEKAGYKPEENIIIGNTCLYGATSGEAYFNGRAGERFGVR
                     NSGVHAVVEGTGDHGCEYMTGGRIVVLGSIGRNFAAGMSGGIAYVWDPKNVHTQYVNM
                     ELVDIEAVTEEKDVDELLHMITRHKEYTGSARAAEILADWKTQLKNFRKIIPGEYKKA
                     LAALADEAQKENEMVDESKLKTVAEGNPKGAKNG"
BASE COUNT          886 a         1299 c         1231 g         1083 t
ORIGIN      
        1 aggaactcca ttgcaaaatg aacacctttc agctcacggc cgggaatcgg caggtcgcgt
       61 ggagcttccg cgccacccgc gagtataacc gcgtcaaagt ctttcacgag ttgttctgcc
      121 ggaatatctt tgccgactgt aacgccagtc ttgatgacca gcccttcagc gaccatctgc
      181 tctgcgcggc ggtcaatctg ccacttctca aatttgaaat ccggaatgcc gaaacggatc
      241 agaccgccga ttttttcatt cttctcaaag agtgtgacgg aatgccccat gcgcaccagc
      301 tgctgcgaag cagccattcc ggcagggcct gaaccaacga ctgctacctt cttaccggtc
      361 ttgtgcgttg gcggttgagg cttaacccaa ccattcttga agccagtatc aatgataaaa
      421 cgttcaatac ccttaatcgc aacagcaggt tcgttgatgc cgagcgtaca tgcaccttcg
      481 cacggagccg ggcacaggcg gcccgtgaac tccgggaagt tgttcgtggt gtgcaggttc
      541 tcgagcgcat cttcatagcg cccacgatat accaggtcgt taaactccgg tatgaggtta
      601 tcgacagggc agcccgtatc gccgtggcaa aaaggtatac cgcagtccat gcagcgcgca
      661 ccctgcacct tggcggtatc agtttcaaac gtgtgctcga attctttata gtgcttcacc
      721 cgatccggta ccggatcgga tttttgcgtt gcgcgggggt actctttgaa acccgttggc
      781 ttacccattc ttagctccct ttgggttgcc ttcagcaacc gtcttcagtt tgctttcgtc
      841 gaccatttcg ttttctttct gcgcctcgtc ggccagtgcc gccagcgctt tcttgtactc
      901 accaggaatg atcttgcgga agttcttcag ttgtgtcttc cagtcggcaa gaatctcggc
      961 agcgcgagct gaacccgtat attctttgtg gcgggtgatc atgtgcagca gttcatcgac
     1021 gtctttttct tctgtaacag cttcgatgtc gaccagctcc atgttcacat actgggtatg
     1081 cacgttcttg gggtcccaca catatgcgat accgccagac ataccagcag cgaagttacg
     1141 gccaatgctg ccgagtacga cgatgcgtcc gccagtcata tattcacatc cgtgatcgcc
     1201 tgtgccttcg acgaccgcgt gcacgcctga gttgcggaca ccaaaacgtt cgccggcacg
     1261 accattgaag taggcttcgc cggaagtagc accgtagagg caggtgttac caatgatgat
     1321 gttctcctct ggcttatagc cggctttctc tggcagacgg acaacgaggc gcccacccga
     1381 gagacccttg ccgcaatagt cgttcgcctg gcctgagagc ttgagcgtaa cacccttcgc
     1441 caggaagcag ccaaagctct gtccggctgt accggtcatc tgtatctgaa ttgtgtcgtg
     1501 cggcaggcct tcgtcaccgt agcgcttcgc cacttcacca gagagcattg cgccaactgt
     1561 acggtcgacg ttagtgacag gcagctggat gtttaccggc tccttgcgat caagagctgg
     1621 tttcgccatc tcaatgattc ggttgtcgat ctgcttctcg atctcgtggt tctgcgcttt
     1681 ggtgcggtaa aggtcggttt tgtataccgg cttctgcatc gtcagcagct tcgacaggtc
     1741 gaggccgcgc gccttccagt gatctttcgg ccttgaaggt atgagcttct cggtacggcc
     1801 gaccatatcg gtcagtttgc ggaagccaag cttcgccatg tactcgcgga cttcttcggc
     1861 aatgtagaac atgaaattga ctacgtgctc tggcttgccg ttaaacttct tgcgcagctc
     1921 aggatcctgt gtcgccacgc ccaccgggca ggtgttcaaa tggcacttac gcatcatgat
     1981 gcaaccgagc gtcaccagcg gagctgtcgc gaaaccaaac tcttctgctc cgagcagcgc
     2041 ggcaataacc acgtcacggc cggtcttcat ctggccatcg gcctggacat agacacggtc
     2101 acgcaggccg ttgaccacca gtacctggtg tgtctcagca aggccgagct cccacggtgt
     2161 gcctgcgtac tgtatcgagc tgatcggcga agcacccgta ccaccgtcat ggccactgat
     2221 cagaatgtga tctgcatgcg ccttggccac gccggccgcc acagtaccga caccgctctc
     2281 agaaaccagc ttcacgctga tgcgcgcacg cgggttgata ttcttgaggt cgaagatcag
     2341 ctgtttgagg tcttcgatcg aatagatgtc gtggtgcggc ggaggagaaa tgagcgtcac
     2401 acccggggtt gagtggcgca ccttaccgat gatacggtca accttgaaac caggcagctg
     2461 gccaccctca ccaggttttg cgccctgcgc catcttgatc tgcagctcgt cggcattgat
     2521 caggtagttc gcggtgacac caaagcgacc cgaggccacc tgcttgatag cggagcgcag
     2581 actgtcgccg ttcggcaatg tcacgaaacg ctcggcacct tcgccgccct cgcctgtgtt
     2641 actcttgccg ccgatgcggt tcatcgcaat cgccaggttc gtatgcgctt cccacgaaat
     2701 agagccgaag ctcattgcgc cggttgcgaa gcgtttgacg atttctttcg ccggctctac
     2761 ttcttcgagc ggaataggat ttgccgcgta atcgaattca aacagcgagc gcagggttac
     2821 ccggcgcttc gactggtcgt caatggccgc ggtatattct ttgaatgttt tgtagtcaga
     2881 gttgcgcgtg ctgatctgca gcttggcaac cgtcgtcggg ttccagaggt gtgcttcgcc
     2941 gtcgacgcgg tagtggtaga gaccaccact cggcagaatg ccttctttca gcaggccaaa
     3001 ggcttcttcg tggcggcggc gtgcctcttc ttcgagcatc tcgaggctga cgccttcgac
     3061 gcggctcggc gtacccgtga agtactgctc cacaacttcg gtgtcgagac cgacagcttc
     3121 aaagatctga gccccgcagt atgactgcag ggtcgagatt cccattttac tgaaaatctt
     3181 gaacaggccc ttaccgatcg ccttgcggta gttcttctct atgtaggttt cttctttaag
     3241 ttcaggcatg aacccgatct tgtagagatc gtgcagcgtc tcgtacgcca ggtacgggtt
     3301 gattgcgttc gcgccgtagc ccagcaacag ggcaaagtgg tgcacctcgc ggggctcgcc
     3361 actttcgagc acgataccgg tgcgagttct caggcccgcg cggatcagcg catggtgaac
     3421 ggcagcaacg gctatcagac tggggatcgg ggcaacttcg cggctgacac cacggtcgct
     3481 caggatgaca atgttggcgc cttgtcgtac cgcgtcgcag acgctggcac agatcttgtc
     3541 gagctgcgta cgcaggtcgt tcttctttgc cgggttgaag tgtagcgaaa atacctttgc
     3601 cgaaaactgg cctttggcaa tttcaatcaa ctgctgcagc tggttgtttt ccagaatcgg
     3661 gtgcggcagc tcgatgcggt gcgcgtgcag cggcgtctcg tcgagcaggt tctgctcggg
     3721 accgatatag gtcgtgagct ccatcacgag ctcttcgcga atcgcgtcga ccggcgggtt
     3781 ggttacctgc gcaaacagct gcttgaagta gcggaacaac aactgcggct tttcagaaag
     3841 taccgcaaga gctgcgtccg tacccatcga acccacggct tcctggcccg tgactaccat
     3901 cggcttcatc acggtgagca aatcttcttt cgtgtagccg aagatgcgct ggcgctcgag
     3961 catcgttttg tgctcaggag tgtgaccgtc tttgggtgcc ggcaggtcgg caaacttgat
     4021 catgttttct ttgacccatt tgccgtaggg ctgctgcttg cagattttgt ctttcacttc
     4081 gtcatcagaa atgatgcggt gctctgaaag atcgatcagg aacatgcgtc ccggttcgag
     4141 tttgccgctg agaacaatct cttcgggagg cacttcgagc acacccattt cagaagccat
     4201 gatcacgatg tcgtctttgg tcttgatata gcgtgcaggg cgcaaaccgt tacggtcgag
     4261 cgtcgcaccg atcacgttac catcagtaaa cgccaccgca gccggaccat cccacggctc
     4321 catcatggtg gcgtggtatt cgtagaacgc ccgacgctct tctgacatca ggtcgttctt
     4381 cgaccatgcc tcgggaatca tcatcatgat cgcgtgcggc aggctgcggc cgctcatcac
     4441 tagcagttcg agtacggtat caaaggtagc cgagtcagac tggccttcca tgacgatcg
//
LOCUS       DAOQJF010000274         4474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11649_length_4474_cov_3.676850, whole genome shotgun sequence.
ACCESSION   DAOQJF010000274 DAOQJF010000000
VERSION     DAOQJF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11649_length_4474_cov_3.676850"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PLF85_13015"
     CDS             complement(<1..1117)
                     /locus_tag="PLF85_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNE20639.1"
                     /translation="MAATRASTSFIFITILLDVIGFGLIIPVMPKMVSKFTSSDVETN
                     YWYMVMTASYGIMQFFFSPILGALSDKFGRRPVILISIVGLGIDFILQGIAWSIPVLF
                     AARVLGGITGASFSVGSAYIADITSPEERAKGFGMIGMAFGIGFILGPMLGGFLGDYR
                     PELPFFAAAAFSLLNALYGMFVLPESLPRHLRTPFSLRKANAFSALKGLAELKGLGIF
                     ILAIALTNLAQFMMHSVWVRYTETRFLWTPKDNGVALFAVGMSAAIVQGGLLGVLIKK
                     FGEVSIAKVGLFFATLEFLLFGLAPQGFYLYIIMAFCILANAAGPALQAHVSRCVPAH
                     KQGIAMGSLSSIGSIAQVLAPFMGLTLLSKYSHAPGAL"
     gene            1361..>2561
                     /locus_tag="PLF85_13020"
     CDS             1361..>2561
                     /locus_tag="PLF85_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HNE20640.1"
                     /translation="MRKWPTILLLLAAWPIAAAQKINWEEIEWQLLKTQHFDVYFPEG
                     YNHLGQKTAEYAEAANILLSEKLKHRLSMVTPIFVYPSHGHFQSTNIIPFAIDEGTGG
                     FTERIKRRVVVPYLGSNDEFRHVVVHELVHAFQYDILLGQTMGSSFSIVQGGQPPLWF
                     VEGMAEYFSLGWDHTADMQMRDAALTDTFPTLEMMTEYRVQTGFIFYKGGQSVMQYIA
                     ETYGENKIGEMLRDLRDLRGIEDAVKTNLGITFEDFDAGWRRWVKRKYFPLVNKKYDD
                     EEGPLLTRHLNDESLINLHPAISPDGSKAVYLTARGFYPVMVMRDIKDVKLDRDYTLN
                     KKKDPTEDEKILLKSSDNPTFFQLHLMTNRITFTPDSKRIFFSARSQGKDRLILFDIE
                     KREIVQRL"
     assembly_gap    2562..2661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2662..4473
                     /locus_tag="PLF85_13025"
     CDS             <2662..4473
                     /locus_tag="PLF85_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNE20641.1"
                     /translation="KDRLILFDIEKREIVQRLTPPVDMVMQPRLSPDGKHAVFVGVTL
                     GVTDIYRIDLATQKVEKLTHDAFSEKDPALSGDNRLVVYSSNRNADNNLESADYHLYE
                     LDLQSKQTRQLTTDAEKQLSAQFYERGSNDRILYVSNHTGIHNVYLLNRKTLQKKKLT
                     DTGGGALDAQITYNEEKNAKGELQDRRIIYTQFRQQGFDIALREVKPGDEKFYTDDNT
                     VHTYKAFALPRYDATGKPMSLEPYRARVGPDFLFFLLGYGAYSGGSYFAGMVAMTGSD
                     LTGNHNFSIVANFLTNGLGIFNFDYGYLKKRVKLHWGAYRTTFAISPVNLIDLTSLNG
                     LFYYPPYIGSVMKIGGYFGMEYPMTPFDSLLLQFNMARAEESFLYPRTVPDERIRPDT
                     FTNMYSVQYGIRHNNVLYSVIGPLKGWHASYVGEQSMNVSGRDYIFNRNTVDARLYIH
                     IWQRYIFAGRVYAATTNGPDQQYFPWILGGFNTIRGYDFMSLRGYHAFFFNLEMRFPM
                     LDALAFGFPAPWVIRGFSMVMFVDGGSAFDSTRTWRGFDYGKNEFQDLKMSYGLGARM
                     ILFPGLMLKIDWATPWTWRSSRPIGDWQGVFSIGYEY"
BASE COUNT         1057 a         1220 c         1191 g          906 t
ORIGIN      
        1 gcagcgcacc cggggcatgc gaatatttcg aaagcagcgt gagccccatg aagggcgcca
       61 gcacctgtgc aatactgcca atcgacgaga gtgaacccat cgcgataccc tgcttgtgcg
      121 ccggcacgca gcgtgagacg tgagcctgca acgcggggcc tgctgcattc gcgagaatgc
      181 agaacgccat gatgatatac aggtaaaaac cctgcggagc caggccgaat aacaaaaatt
      241 ccagcgtggc aaaaaacaat cccactttgg cgatgctcac ttcgccgaat tttttaatca
      301 gaacgccgag caggccgccc tgtacaatcg ccgccgacat accgacggcg aagagtgcca
      361 cgccgttatc tttcggcgtc cagagaaagc gggtttcggt atagcgcacc caaacggagt
      421 gcatcatgaa ttgcgcgagg ttcgtcagtg cgatcgcgag tatgaagatg ccgagaccct
      481 tgagttctgc gagacctttc agggcagaaa aagcattcgc cttcctgagg gaaaacggcg
      541 tgcgcaggtg gcgcggcagc gactcgggta aaacgaacat gccgtagagc gcgttgagca
      601 gcgagaaagc cgccgcggca aagaacggca gctcggggcg gtagtcgccg agaaagccgc
      661 cgagcatggg cccgagaata aaaccgatac cgaacgccat gccgatcata ccaaaacctt
      721 ttgcgcgttc ttcgggtgaa gtgatgtcgg cgatgtaggc gctgccgaca gaaaacgagg
      781 ccccggtgat accgccgagc acgcgcgcgg caaaaagtac cggtatcgac cacgcgatgc
      841 cctgcagaat gaaatcgata ccgagaccaa cgatcgagat cagtatcacg gggcgccggc
      901 cgaacttatc gctcagagct cccaaaatgg gagagaaaaa gaactgcatg atgccgtagc
      961 tggcggtcat caccatgtac cagtagttgg tttccacgtc actgctggtg aacttagaca
     1021 ccattttcgg catgaccgga ataatcaggc caaagccgat gacatcgagc aggatggtaa
     1081 taaagataaa agatgtactc gcgcgtgtcg ccgccatgct gccatcgccg gagcgcgcga
     1141 tatgcggtcg aatggttggg caggtatgga atattgcttt aaaacgcgtg cgcagccagg
     1201 ccgttcggag gatcagttag gtagacgctg gaagtgcaca cactacataa tgacacaaat
     1261 gtcattaaga tgccaaaaaa agtgcatttt ccgcccgggc tgctagtcgc tgattcaacc
     1321 ttgacgcaat gatcggccgc gcgaccctgc ggtacactgc atgcgcaagt ggccgaccat
     1381 actattacta ctggccgcct ggccgatcgc cgccgcgcag aaaatcaact gggaagaaat
     1441 cgaatggcag ctgctgaaaa cacagcactt tgacgtgtat ttccccgagg ggtacaacca
     1501 cctggggcag aaaacggctg agtatgctga agcggcaaat atcctgctga gcgagaaact
     1561 gaagcaccgc ctgtcgatgg tcacaccgat ctttgtctac ccttcccacg ggcacttcca
     1621 gtcgaccaac atcatacctt ttgcgatcga cgagggtacc ggcggcttta ccgagcgcat
     1681 caagcggcgc gtggttgtac cgtacctggg tagcaacgac gagtttcggc acgtggtcgt
     1741 gcacgagctt gtgcatgcgt tccagtatga tattctgctc ggccagacca tgggcagcag
     1801 cttctcgatt gtgcagggtg gtcagccgcc tctctggttt gtcgagggta tggcggagta
     1861 cttttcgctc ggctgggatc acaccgcaga catgcagatg cgcgacgccg cgctgaccga
     1921 tacttttccc acgctcgaga tgatgaccga gtaccgggtg cagaccggtt tcattttcta
     1981 caagggcgga cagtcggtca tgcaatacat tgccgagacc tatggcgaaa acaagatcgg
     2041 tgaaatgctg cgcgacctgc gtgacctgcg cggtattgaa gatgccgtga aaaccaatct
     2101 cggcatcacg ttcgaagatt ttgatgccgg ctggcggcgc tgggtgaaac gcaaatattt
     2161 tccgctcgtg aacaagaagt acgacgacga agaaggtcct ctgctcacgc gccacctgaa
     2221 cgacgagtct ctcatcaacc tgcacccggc aatttcaccc gacggtagca aggccgtgta
     2281 cctcaccgcg cgcggttttt atcccgtgat ggtcatgcgt gacataaagg atgtcaaact
     2341 cgaccgcgac tacacgctca acaagaagaa agaccccact gaggacgaaa agatactgct
     2401 caaaagctca gacaatccga ctttttttca gctgcacctg atgacgaacc gcatcacctt
     2461 tacacccgac tccaaacgca tcttcttttc agcgcgcagc cagggcaaag accggctgat
     2521 tctgtttgat attgagaagc gcgaaatcgt acagcgcctg annnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn naaagaccgg ctgattctgt ttgatattga gaagcgcgaa
     2701 atcgtacagc gcctgacacc gcctgtcgac atggtgatgc aaccccggct ttcgcccgac
     2761 ggcaagcacg cggtgttcgt gggagttacc ctcggtgtca cggacattta ccgtatagac
     2821 ctggcgacac agaaggttga aaagctgacg catgacgcgt tttcagagaa ggatcccgcc
     2881 ctttcgggcg ataaccgcct cgtcgtctat tcttcgaacc gcaacgccga caacaacctg
     2941 gagagcgccg actaccacct gtacgaactt gacctgcagt cgaagcagac acggcagctg
     3001 acgaccgatg cagagaaaca gttatctgca cagttctacg aacgcggctc aaacgaccgc
     3061 atactctatg tctcgaacca cacgggcata cacaatgtct acctgctcaa tcgcaagacg
     3121 ctgcagaaga aaaaactgac cgatacaggt ggcggtgcac tcgatgcaca gataacctac
     3181 aacgaagaaa aaaacgccaa gggcgaatta caagaccgtc gcatcatcta tacccagttt
     3241 cgtcagcagg ggtttgatat tgcgctgcgc gaagttaaac ccggcgacga aaagttttac
     3301 accgacgaca acaccgtcca tacctacaag gcgtttgcgc tgccgcgtta tgatgcgacc
     3361 ggcaagccga tgagcctcga accttaccgg gcgcgcgttg gccctgattt tcttttcttt
     3421 ttactggggt atggggcgta ttcgggcggt agctatttcg cgggtatggt cgcgatgacc
     3481 ggttcagacc ttacgggtaa ccacaacttc agcatcgtcg cgaactttct cacgaatggc
     3541 ctgggcattt tcaatttcga ttacggctac ctgaaaaaaa gggtcaagct gcactggggt
     3601 gcttaccgca cgacatttgc gatctccccc gtgaacctca ttgatctgac ttcgttgaat
     3661 ggactttttt actacccgcc ttacataggc tcagtcatga agatcggcgg ttacttcggt
     3721 atggagtacc cgatgacgcc attcgattcg ttactgctgc aattcaatat ggcgcgcgct
     3781 gaagaaagtt ttctttaccc gcgcactgta cccgacgagc gtatcaggcc tgacactttc
     3841 accaacatgt acagcgtgca gtacggcatt cgacacaaca acgtactcta ctcggtcatc
     3901 gggccgctca agggctggca tgcgtcgtac gtcggcgaac agagtatgaa tgtgtcgggc
     3961 cgcgactaca tcttcaaccg caatactgtc gacgcccgtc tctatataca catctggcag
     4021 cgttatatct tcgcgggccg ggtttatgcc gcgacgacca acggtcccga ccagcagtac
     4081 tttccatgga ttcttggcgg cttcaacacg attcgcggct acgacttcat gtcgttgcgc
     4141 ggctaccacg cattcttctt caacctcgag atgcgctttc cgatgctcga cgcgctggcg
     4201 tttggttttc cggcgccttg ggtgattcgc ggcttttcga tggtcatgtt tgtcgacggg
     4261 ggcagtgcgt tcgacagcac gcgcacctgg cgcggtttcg attacggcaa gaacgaattc
     4321 caggatctga aaatgtcgta cggcctcggg gcgcgcatga tactttttcc cggtctgatg
     4381 ctcaagatcg actgggcgac accctggacc tggcgcagct cgcgaccgat cggcgactgg
     4441 cagggtgtct tctcaattgg ttacgagtac tgac
//
LOCUS       DAOQJF010000275         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11772_length_4441_cov_5.338121, whole genome shotgun sequence.
ACCESSION   DAOQJF010000275 DAOQJF010000000
VERSION     DAOQJF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11772_length_4441_cov_5.338121"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLF85_13030"
     CDS             <1..183
                     /locus_tag="PLF85_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20642.1"
                     /translation="AAGTVTACTTPAQIHINSSNFAYLVCNGENKVNAYSVSGSSLNL
                     VGSYSTGTQPTSVAGY"
     gene            complement(199..501)
                     /locus_tag="PLF85_13035"
     CDS             complement(199..501)
                     /locus_tag="PLF85_13035"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE20643.1"
                     /translation="MAAKKSIIITQTAQSQRLSILAYWFDRLGHGRYSAVLARDFEHC
                     LDLIQKFPQIGRKIQRTGYLGFTIRDFLIVYELTTNEIVIHQFWDLRQGPTSSVVQ"
     gene            complement(480..722)
                     /locus_tag="PLF85_13040"
     CDS             complement(480..722)
                     /locus_tag="PLF85_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20644.1"
                     /translation="MSLAEIKELIHEQVENSSDPEVLRLVNELLAHDHLPRREIYLTA
                     DQQQALAKSADDQRAGRTITRNDAAQQVNDWLQKNP"
     gene            851..1867
                     /gene="purM"
                     /locus_tag="PLF85_13045"
     CDS             851..1867
                     /gene="purM"
                     /locus_tag="PLF85_13045"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNE20645.1"
                     /translation="MDYKTAGVDTEYAQKLIHNLKGRISGTHKVPNVGEIVGQYGGFA
                     GRFQPVAIPGYDIVAATDGVGTKTQLLRRFNYLEGIGQDLVAMCVNDLFCSGATPAFF
                     LDYIACGKLDESWYNRVLSSIADACAATPMALMGGETAEHPGVMPDDDFDLAGFAVGF
                     IARDHRLPKPELQREGDLLVGLSSSGLHSNGFSLVRKIFAELEKNSPAEFQTMAADRE
                     KFIATWLAPTRIYKEIPTLLQQAEIKGIAHITGGGIHENLPRAIAEGLGAVIENAEPF
                     RLQAAGLLEQHVAREKLFHTFNMGTGLILIASEGELEKIRKVYPEAAVMGRLAKGQGV
                     SLKL"
     gene            1926..2606
                     /locus_tag="PLF85_13050"
     CDS             1926..2606
                     /locus_tag="PLF85_13050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNE20646.1"
                     /translation="MKQMNRAGLIALAITTTISFSACNRADVSSEKGKASYAIGYQVG
                     RALKSQGAEVDPATIAAAIKDTMAGNELRMTEDEMRQAIQALQSRNQGGDPKVAAENA
                     AKGKEYLDANKAKPGVQVTASGLQYKVISAGSGASPKATSTVKVHYRGTLIDGTEFDS
                     SYKRGQPAEFGVSQVIRGWTEALQLMKKGAKYQLTIPSELAYGARNSGTIPANSVLNF
                     EVELLDFK"
     gene            complement(2635..3639)
                     /locus_tag="PLF85_13055"
     CDS             complement(2635..3639)
                     /locus_tag="PLF85_13055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE20647.1"
                     /translation="MRSVGFVFLLFPVALVPTNCSNSWGKFWEVPPEDPLAKCRSAVS
                     GDSISMICVPANTTGFSRGNASVPAGGTPIHTVASITGFAMGKFEVIFSEWSAIRTWA
                     LSNGYTIPNAGTQGGGTGAKTDQHPATSMDWRDMIVWCNAASEKAGLTPVYYTDAAFT
                     SPLRVSGGAVNTTPGAQDNPYVRWAANGYRLATGAEWEYAARYIDGSTYMRGDAPSGW
                     SDNNLANNVIDQAEKNVVSWSDNNSGGGTKPVGQLQPNSLGIYDMSGNVFELVFDWNG
                     AYTTNSPYTDADSFGPASGTSRDVRGGGYGGEYGVSNRGTTTPDSPAAERGFRVVRRP
                     "
     gene            3971..4258
                     /locus_tag="PLF85_13060"
     CDS             3971..4258
                     /locus_tag="PLF85_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20648.1"
                     /translation="MKKTIMVLAAMVFAGSVASVQVVPSENDVKGCARLGEVAAGNKF
                     MQMDRETAEKAVREQAKEMNADKVFLSVTSHTHPKLGKQYTAKAVAWKCDK"
BASE COUNT         1119 a         1259 c         1153 g          910 t
ORIGIN      
        1 gccgccggta ccgtcaccgc ctgcacgacc cctgcgcaga ttcatatcaa cagcagtaac
       61 ttcgcctacc tcgtctgcaa cggcgaaaac aaggtaaacg cgtactcggt ttctggctca
      121 agccttaatc tcgtgggtag ctacagcaca ggaacgcagc ccaccagtgt ggcaggttat
      181 tgaaccacat cgactgcttc actgaacaac cgaactggtt ggtccttgcc gcaaatccca
      241 aaactggtga atgacgattt cgtttgtcgt cagttcataa acgatgagaa aatcgcgtat
      301 cgtgaagccc aaatagcctg ttcgctgaat cttgcggccg atctgcggaa atttctgaat
      361 cagatccaga cagtgttcaa agtctctcgc cagcactgca ctgtagcgac catggcccag
      421 gcgatcaaac caataggcca aaatactcaa ccgctgactt tgagctgtct gggtaatgat
      481 tatggatttt tttgcagcca atcatttacc tgctgggcgg catcattgcg cgtaatggtt
      541 ctacctgccc gttgatcgtc tgcagatttc gccagggctt gctgctggtc tgccgtcaaa
      601 tagatttcac ggcgcggcag atgatcatgc gctagcagtt cattcacgag cctcagaacc
      661 tcaggatctg aggagttctc aacttgctcg tgaatcagtt ccttgatttc agccagcgac
      721 ataaggcatg ttacgattga aatataactc cgtcaacccc attagaggtg tcaactactg
      781 gctcgatggg gtatcgacta tctaaaccaa atttccattt tcgccctgtc ttgccgcgca
      841 agacttcccc atggattaca agacagccgg cgtcgatacg gaatacgcgc agaagttaat
      901 ccataacctc aaaggccgca tcagcggtac gcacaaggtg cccaatgtcg gcgagattgt
      961 cggtcagtac ggcggttttg ccggccggtt ccaaccggtg gctattcccg gttacgacat
     1021 cgttgccgcg acagacggtg tcggcaccaa aacgcagcta ctccgccgct tcaattacct
     1081 cgaaggtatc gggcaagacc tcgtggcgat gtgcgtcaac gacctgttct gctcgggtgc
     1141 tactcctgca ttttttctcg actacattgc ctgcggcaag ctggacgaat cgtggtacaa
     1201 ccgcgtactc agttcgatcg ccgatgcctg cgctgccacg ccgatggcgc tcatgggtgg
     1261 cgaaaccgcc gaacaccccg gcgtgatgcc cgacgacgat tttgacttag caggcttcgc
     1321 agtgggattt atagcgcgcg accaccggct accgaaaccg gagctgcagc gcgaaggcga
     1381 tcttctggtt ggtctctctt cgagcggcct gcactcgaac ggcttttctc ttgtacgcaa
     1441 gatctttgcc gaactggaga aaaatagtcc agcagagttc cagaccatgg ctgccgatcg
     1501 cgaaaagttc atcgcaacat ggttagctcc cacacgtatc tataaagaaa ttccgacgct
     1561 gctacagcag gcagaaatta agggaatcgc gcacatcacc ggtggtggca ttcatgaaaa
     1621 tctgccgcgc gcaatcgccg aaggactggg ggctgtcatc gagaatgctg aacctttcag
     1681 gctgcaggca gccggcctgc tcgagcagca tgtcgcacgc gagaaacttt ttcatacatt
     1741 caacatgggc accggcctca tcctgattgc atcagaaggc gaactggaaa aaatccgcaa
     1801 ggtctacccc gaagccgctg tcatgggaag attggccaag ggccagggcg tctcgctcaa
     1861 gctttgacct gttcagcgcc aaaaccttgt ttacgcggcc cgcgtgcgcc cggcttaacc
     1921 gctcaatgaa acaaatgaac cgggcgggac ttattgctct cgccatcacc accaccatca
     1981 gcttcagtgc ctgcaatcgt gcagatgtca gctccgaaaa gggcaaagca agttatgcga
     2041 tcggctacca ggtaggccgc gcactcaaat cgcagggcgc ggaagtcgat ccggcaacca
     2101 tagcagccgc catcaaagat acgatggcag gcaatgaact gcgcatgaca gaagatgaaa
     2161 tgcgccaggc aatacaggcg ctgcaatcgc gcaaccaggg cggcgacccc aaagttgcag
     2221 ccgagaatgc agcaaaaggc aaagagtacc tcgatgccaa caaggccaag cccggtgtcc
     2281 aggtcaccgc ttctggcctg cagtacaaag ttatcagcgc aggcagcggc gcttcaccca
     2341 aggcaacaag caccgtcaaa gtacactacc gtggtacgct gatcgacggc actgaatttg
     2401 acagctcgta caaacgtggc caacccgcag aattcggcgt cagccaggtt atccgtggct
     2461 ggaccgaagc gctacagctg atgaaaaagg gtgcaaagta ccaactgaca attccgagcg
     2521 aactcgcgta cggtgcgcgg aacagcggca caatacccgc aaactcagtg ctcaactttg
     2581 aagttgagct gctggatttt aagtagtttg aattcttagg tgcgctttgc gcacctaagg
     2641 acgcctgacg acgcggaagc cgcgttctgc cgcgggtgaa tcaggcgtgg ttgtaccgcg
     2701 gttcgagaca ccgtattctc ccccataacc accaccccta acgtccctcg aggtacctga
     2761 tgccgggcca aacgaatctg catctgtgta aggtgaattg gtggtgtagg caccattcca
     2821 gtcaaacacc agttcgaaga catttccgct catgtcgtag atgcctagag aattaggctg
     2881 caattgccct actggtttag ttccgccacc agagttgttg tcggaccaag aaactacgtt
     2941 cttctccgct tgatcaatca cattatttgc caggttattg tcgctccagc cactgggtgc
     3001 atcaccacgc atgtaagtcg aaccatcgat gtatcgcgct gcgtattccc actcagcacc
     3061 tgtcgcgagg cgataaccat tggccgccca cctaacgtac ggattatcct gggcgccggg
     3121 agttgtattc acggcaccac cagaaacgcg cagcggcgaa gtgaaggccg catcagtata
     3181 gtagactggg gtaagtcctg ccttttcaga tgcggcgtta caccagacaa tcatgtcacg
     3241 ccaatccatg ctcgtcgctg gatgctggtc agtcttagcg ccagttccgc ccccctgtgt
     3301 accggcattg ggaatagtgt atccatttga cagcgcccag gttctgattg ctgaccactc
     3361 ggaaaagatg acctcaaatt ttcccatggc aaaacctgtg atggaggcga ccgtatggat
     3421 cggcgtaccg ccagctggaa ctgacgcgtt acccctgcta aaccccgtcg tgtttgccgg
     3481 cacgcagatc atgctgatcg aatcgcccga tacggcgctg cggcatttgg cgagcgggtc
     3541 ttcgggcggg acttcccaga acttacccca actgttgctg cagttggtag gtactaaagc
     3601 cacgggaaaa agcaagaata caaacccgac agagcgcatc gctccaaaat ctgtataccc
     3661 ccaggcatgt cgaacgagat tccaccaaac ctgcggcaac gaaaaccaga agaatatccc
     3721 atcaaatcaa agaaacatga agccaactgc acttcgaccg aatgcagccc aagagtagcg
     3781 cggcggagct acagtagttt caaaactccg tagtaacacg cagataatac cgtggcggct
     3841 ctgatgacaa atcgccgaaa atgggcgata ctcatagcaa caagagggca taaccctcaa
     3901 gcggcggcgg gcacagaaca agaataaatc ccccgccaaa aagataccgc gggtatcact
     3961 ggaggacaac atgaaaaaga caattatggt tctggcggca atggtatttg ccggttctgt
     4021 ggcttcggta caggtcgtac ccagcgaaaa cgatgtgaaa ggttgcgcgc gtctcggtga
     4081 agtcgcagca ggcaacaagt tcatgcagat ggatcgcgaa accgctgaga aggccgtgcg
     4141 cgaacaggcg aaagaaatga acgctgataa ggtgttcctc agtgtcactt ctcacaccca
     4201 cccgaaactc ggcaaacagt acacagcgaa agctgtcgcc tggaagtgcg ataagtaagc
     4261 actggccgca gggctgcccc aggcggccct gcaaaccctg gccgggtttg ggtttgcgcc
     4321 ctgtctcaaa ggggcaatct gacccatggc agataagaaa cgcgacgaca tcctcgtcga
     4381 aacgctcaac cccgacatgg ggtatttctt cagaacgagc aagaaactct acgaaacccg
     4441 g
//
LOCUS       DAOQJF010000276         4431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11809_length_4431_cov_5.309415, whole genome shotgun sequence.
ACCESSION   DAOQJF010000276 DAOQJF010000000
VERSION     DAOQJF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11809_length_4431_cov_5.309415"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLF85_13065"
     CDS             complement(<1..464)
                     /locus_tag="PLF85_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20649.1"
                     /translation="MKKTDWAIRDQKLQMLDAVKRAQDKYAFLDDYRYALEKDLPTVE
                     AAIRKYGDELRRPLYRFFAGDILEDVAQTAHDDFLRSLRQYIAEGRLTANAGVSDRLL
                     ESLRQAIGRNIETLVSGSDADMIQRAREFKAIAAESANAAVVAQTQMTQLKI"
     gene            complement(461..1141)
                     /locus_tag="PLF85_13070"
     CDS             complement(461..1141)
                     /locus_tag="PLF85_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20650.1"
                     /translation="MESTYCTVAEVTTYAAANGELHWSQLSAQDLTGAINNASGYLVG
                     DTSITVDGFADDVQRITAGSVFTIASDSTSTEHTILSTKYDSGTVEISFTPGLAEKVA
                     DNDVVNIQATEATAFQIRCVVQACRDIVRYHAQRWSDGTLWLETDALLKKANIIQALY
                     LARVFQMRDSAENISALTGGSYSDGVVSIETLSAPGLCPDAQDIVATVLRENKDILMP
                     KRGIYAGR"
     gene            complement(1143..1391)
                     /locus_tag="PLF85_13075"
     CDS             complement(1143..1391)
                     /locus_tag="PLF85_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20651.1"
                     /translation="MSRTKEAPEGQKEEAPNVDVKGFLAKCDTRNKSGEALREAYKSL
                     EQFQEVARSYGRVVTVNAGRVDHNIPADQLPWMIALIK"
     gene            complement(1406..2575)
                     /locus_tag="PLF85_13080"
     CDS             complement(1406..2575)
                     /locus_tag="PLF85_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HNE20652.1"
                     /translation="MAIANTLTGLVPTMYRAMHTVSRENVGALPGATIDASAAMAAKD
                     QTVRTPVAPAATAESITPAMAVPETGGQTISYIDMTLSKSKAVPIPFSGEEELSLGDM
                     YQVVMEQRIAQAIRTLVNEMESDLCTAAYTGASRAYGTPATTPFASSLADAAEIKRIL
                     DVNGAPGMNDPNYRSLVINSAAGVNLRKLTNLMTVNANGSDQTLRNGILLPVVGFGVR
                     ESAGVVSHTKGAGTGYDVNNASGEAVGQTTITLDGGTVNSTGIKAGDIVTFATDANNK
                     YVVNTGLTSTTGDIVIGNPGIQVLVADTTEMTIGDSYTANFGFSKNALVVATRAPAMP
                     RMGDLSVDVQQVTDPVTGIVFTFAMYRGYHAVRFEVSAVWGVKAVQSEHIAVLIG"
     gene            complement(2585..3322)
                     /locus_tag="PLF85_13085"
     CDS             complement(2585..3322)
                     /locus_tag="PLF85_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20653.1"
                     /translation="METTQTAQPAETTTQETFAFVDPQTRKSLALPRQVGEVDLQKLI
                     EGVIAKSNEDFRKKYKSEIENLNSQLSESEKMKARLDELESLTIPAKEREAKQVQAAI
                     EKAQKEAQAKAEAATKYEQAFKSEKLSTELFKAASKHQNIRNLDQVATLFQAQCTPAV
                     VEENGEWKVMAKFNGAELPVSEALGQWLSLEENSNLLTPQLAPGAGSTGGTRTGASAS
                     QVRRADFNAMKPAQQMDFIIQGGKVID"
     gene            complement(3322..>4431)
                     /locus_tag="PLF85_13090"
     CDS             complement(3322..>4431)
                     /locus_tag="PLF85_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20654.1"
                     /translation="DSPNFNPEEIQTEADRRAMGLQPYPCVYYPYQIRDYAVGEDGAL
                     LWVLLDDSKVDNSNPLKEAEKKTIYRLWTAQVSQEFEIKKKSQNVDDWEVIAGEPMPH
                     PVGYVPFHFVNWRDLNDDMVSDSPMEDIAILSEQIYNVLSLLDEMLHTGTFKTLFFPV
                     QKRGDLQEDIQKQGLFDIAVVEYYPAEGKEPTFQGPGLSDVGPFIEALKLYSYEIFRK
                     VGMDVDRDKSYVQSGAAIGKEFEKVEALLRYGAKAMQETEEFIFRTAGRWLGQDIDEQ
                     VEIEYSKEFQRADIDIKLQRLFNVFNLGLRPIKQMALKGILKETFPSLPEEKIEEMSQ
                     ETEGNAPTWPLPSIDQARAVADAAPSLPTQNEVST"
BASE COUNT          888 a         1198 c         1150 g         1195 t
ORIGIN      
        1 tcaattttta gctgtgtcat ctgcgtctgc gccactaccg cagcgttggc cgattctgcg
       61 gcgatggcct taaactcgcg tgcgcgctgt atcatgtcgg cgtctgagcc actgacaagt
      121 gtctcgatat tgcgcccgat ggcctgacgc aggctttcaa gtaacctgtc agacacacca
      181 gcattagcgg tcaatctacc ctcagctatg tactgacgca gcgaccgcag aaagtcatca
      241 tgtgcagttt gtgcgacatc ttcgagaata tcgccggcaa agaaacggta cagcggcctg
      301 cgcaattcgt cgccgtattt tcggatggcc gcttcgactg ttggcaagtc cttttctaat
      361 gcataacggt agtcgtctaa aaacgcgtac ttgtcctgcg cgcgttttac cgcgtcgagc
      421 atctgaagct tttggtcccg gattgcccaa tcggtctttt tcatcggcct gcgtatatcc
      481 cccgtttcgg catcagtatg tctttgttct cacgcagcac ggtagccacg atgtcctgag
      541 catccgggca cagacccggt gctgacagag tttcgatgga gacaaccccg tcagaatatg
      601 acccgccggt gagcgcgctg atgttttcgg cgctgtccct catctgaaag acgcgcgcaa
      661 ggtataatgc ctgtatgatg ttggcttttt tcagaagggc gtcagtttct agccacagcg
      721 tgccatcgct ccacctttgc gcgtgatagc gtacaatgtc gcggcaggct tggacgacac
      781 agcgaatttg aaaggctgtt gcctcggtgg cttggatgtt cactacgtcg ttatccgcca
      841 ctttctctgc aaggccgggc gtgaacgata tttcaaccgt cccgctgtcg tattttgtcg
      901 ataaaatcgt gtgctctgtg gatgtcgaat cgctcgcaat ggtaaagaca gagcccgccg
      961 tgatgcgctg tacatcatcg gcgaacccgt caacggtgat cgacgtatcg ccgacaagat
     1021 agcccgaagc gttattgatc gccccggtca aatcttgtgc cgataattgc gaccaatgca
     1081 gctcgccatt ggctgccgca taagtcgtaa cctctgccac cgtgcaatac gtcgattcca
     1141 tgttacttaa tgagcgcgat catccatggc agttggtctg ccgggatgtt gtggtctaca
     1201 cgccctgcgt ttacagtaac cacacggcca tatgaccggg cgacttcctg aaactgttca
     1261 agggatttgt acgcttcgcg cagtgcctct cctgatttgt tgcgtgtgtc acacttcgca
     1321 agaaacccct ttacgtctac gttcggggct tcttcttttt gcccttctgg ggcttctttc
     1381 gttctgctca ttttttctcc tttggttacc cgatcagtac tgcgatgtgc tcagactgaa
     1441 cagccttcac accccagacg gcagaaacct caaagcgtac agcgtggtaa ccacggtaca
     1501 tggcaaacgt aaatacgatg ccggtcaccg ggtctgtcac ctgctgaacg tccacagaaa
     1561 ggtcgcccat gcgtggcata gccggtgcgc gggttgccac tacgagagcg tttttgctga
     1621 aaccgaagtt tgcagtgtag ctatcgccga tagtcatctc ggtagtgtct gcaaccagca
     1681 cctgaatacc ggggttgccg ataacgatgt cgcctgtcgt tgatgtgaga ccagtgttca
     1741 caacatattt gttgtttgca tcggtcgcaa aggtcacaat gtcacctgct ttgataccgg
     1801 tagagtttac tgtaccgcca tcgagagtga tagtagtttg ccctactgcc tcgcctgaag
     1861 cgttgttaac gtcgtacccg gtgcctgcgc ctttagtgtg ggaaaccaca ccggcagact
     1921 cgcggacacc gaaaccgact acaggcaata gaatgccgtt gcgcagagtt tgatctgagc
     1981 cgttagcgtt gactgtcatc aggtttgtca gcttgcgcag gttcacacca gcggcggagt
     2041 tgataaccag tgaccggtag ttagggtcgt tcatcccggg cgcgccgttc acgtccagaa
     2101 tacgcttgat ttcggcagcg tctgcgagag acgacgcgaa aggtgtagtg gcgggcgtac
     2161 cgtatgcacg cgatgcgcct gtataggctg ctgtgcagag gtctgattcc atctcgttaa
     2221 cgagcgtgcg aattgcctga gcgatgcgct gctccataac gacttggtac atgtccccca
     2281 gtgacagctc ttcttcacca gagaaaggaa tcggcacggc tttcgattta gaaagcgtca
     2341 tgtcgatgta agaaatggtc tgaccgcctg tctcaggcac cgccatcgct ggggtgattg
     2401 attcagcagt cgcagccggg gccactggtg tcctgactgt ttgatctttt gcagccatgg
     2461 ccgcagaagc gtcgatagtt gcaccgggca gcgcgcctac gttctcacgc gatacagtgt
     2521 gcatcgcgcg atacattgta ggtacaaggc cggtaagcgt gtttgctatt gccattgaaa
     2581 atccttaatc tatgactttc ccgccttgga ttatgaagtc catttgctgt gcaggcttca
     2641 tggcgttaaa gtcagctcta cgcacctgcg atgcagacgc gcctgtcctt gtgccgccgg
     2701 tcgagccagc tccgggggcg agttgtgggg ttagcaggtt agaattttcc tcaagcgata
     2761 accactggcc taacgcttcg ctgacgggga gttcagcgcc gttaaacttt gccatcactt
     2821 tccattcgcc gttttcctca accacagcag gggtgcactg tgcctgaaaa agcgtcgcca
     2881 cctgatccag atttcttata ttctggtgct ttgacgcggc tttgaaaagc tcggtactca
     2941 atttctctga cttaaaagcc tgctcgtact tggtcgctgc ctctgctttt gcttgcgctt
     3001 ctttttgcgc cttttcgatg gctgcctgta cttgcttggc ttcgcgctct tttgccggga
     3061 tcgtgagact ttcaagctcg tctaaacgtg ctttcatttt ctccgattcc gataattgag
     3121 aattgagatt ttcgatctcg ctcttgtact ttttgcggaa gtcctcattt gacttggcaa
     3181 ttacgccttc gatgagcttc tgcaaatcga cctcgcccac ctgccgtggg agtgcaaggc
     3241 tcttgcgtgt ctggggatcg acgaacgcga aagtctcttg tgtcgtggtc tctgccggct
     3301 gtgccgtttg tgttgtttcc attaagtgct tacctcattt tgagttggta gcgaaggtgc
     3361 cgcgtctgcg actgcccgcg cttgatctat gctagggagc ggccatgttg gggcgttgcc
     3421 ctctgtctct tggctcattt cttcaatctt ttcttctggt aggctaggga aagtctcttt
     3481 cagaatgccc ttgagcgcca tttgcttgat aggtctcagg ccaaggttaa acacgttgaa
     3541 aaggcgctgc agcttgatgt caatgtctgc acgctgaaat tccttgctgt attcgatctc
     3601 tacttgctcg tcaatatctt gccccagcca ccggccagcg gttctaaata tgaattcttc
     3661 tgtctcttgc atggcctttg cgccataccg cagcaaggct tcgactttct caaattcctt
     3721 gccgatagcc gcaccgctct gaacgtagct tttatcgcgg tcaacgtcca tgccgacttt
     3781 acggaaaatc tcgtatgaat acagctttag ggcttcgatg aatggcccaa catcagaaag
     3841 ccccgggcct tgaaatgtcg gttccttgcc ttcggcagga tagtactcca cgacggcaat
     3901 atcaaagagc ccctgcttct ggatgtcttc ctgaaggtcg ccgcgtttct gaacggggaa
     3961 aaacagggtt ttgaatgtcc ccgtgtgcag catctcgtca agcagagaaa gcacgttgta
     4021 aatctgttca gacaggatgg ctatgtcttc catcggagag tcagacacca tgtcgtcatt
     4081 gagatcacgc caattgacaa agtggaaagg cacatagccc acagggtgcg gcatcggctc
     4141 cccggctata acctcccagt cgtcaacatt ctggctcttt ttcttaatct caaattcctg
     4201 agatacctgc gcggtccaca gccggtaaat tgtctttttt tctgcttctt tcagcgggtt
     4261 agaattgtct acctttgaat cgtcgagaag cacccacagc agcgcgccat cttcgcccac
     4321 tgcgtagtcg cgtatctggt aagggtagta aacgcacggg taaggctgca aacccatcgc
     4381 ccggcggtcg gcttcggtct ggatttcttc agggttgaaa ttaggcgagt c
//
LOCUS       DAOQJF010000277         4419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11858_length_4419_cov_4.662466, whole genome shotgun sequence.
ACCESSION   DAOQJF010000277 DAOQJF010000000
VERSION     DAOQJF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11858_length_4419_cov_4.662466"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PLF85_13095"
     CDS             complement(<1..1244)
                     /locus_tag="PLF85_13095"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE20655.1"
                     /translation="MYETKDVAQGASGNAATDTDVIVVGGGFSGIGAGIKLREAGFSF
                     VILEKAADLGGTWRDNTYPGIAVDITSFTYSFSFEQNPHWSRLFAPGNELRAYAHHCV
                     DKYGIRDAFRFGVEVDKAVFNEAQGTWQVQAKDGKTWRAKYLIAATGGLTQPKLPDIP
                     GITEFTGKTMHTARWDHGYPLENKRVAVIGTGATSVQLVPSIAPVVKQLHVFQRTPIW
                     ILPKPDREIPQWLQWIFAKVPLAQQSVRNVTTFFTEIVMMLGIVYNKQTPQLIRALEA
                     LCMKHLEEQVNDPAVREKLRPNYGFGCKRPSISNDYLRTFNRSNVELVTDRIERITPT
                     GILTADGCEREIDTLVLSTGFKVYEKGNTPPFEMFGRDGIELGDFWEKNRYQAYEGAS
                     VPGFPNMFLIWGPYSASGSSWFA"
     gene            1434..2045
                     /locus_tag="PLF85_13100"
     CDS             1434..2045
                     /locus_tag="PLF85_13100"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE20656.1"
                     /translation="MAYRRVIGASASEDRLERLIEDRLKPGADKEKIDSRIWDLFGET
                     WAIMFTDLSGFSRNVAKFGIIHFLQTIYESERLLIPIIEDHDGILLKAEGDSLLVIFR
                     NAGKALQAGVAMQRSLEWYNKERPEEEQVLLCVGIGYGKVLRIGDIDVFGHEVNSASK
                     LGEDTAKAYEILVTPAVREVFKDSGEFRFDATEGSHFALHWRD"
     gene            complement(2099..2902)
                     /locus_tag="PLF85_13105"
     CDS             complement(2099..2902)
                     /locus_tag="PLF85_13105"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE20657.1"
                     /translation="MQKVAELLNLSGKSAIVTGGALGIGRAIAKRLAEAGAAVTIADI
                     NQKAIDEAVALISQQGGRVSGCTVDVSSEADIDKLVTHAVKQNGGIDILVNNAGIFPS
                     KLVMQMAKADFERVISTNLTGLFLCTKKVAAQMIAQGKGGRIINITSIDALHPSQVGL
                     AHYDASKHGVWGFTKNVALELAEHKIWVNAIAPGGIVTEGVKEMSSASKMPEGIDMAK
                     VMESFLAKIPMHRMGDPDEIAKAALFLASDMSSYMTGSQIVVDGGALLS"
     gene            complement(2981..3832)
                     /locus_tag="PLF85_13110"
     CDS             complement(2981..3832)
                     /locus_tag="PLF85_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20658.1"
                     /translation="MPPAASLFTSERVVIATRHAKARAIRSAFRQLGLSVRTVRADTD
                     ALGTFSGEVERTRTTLECALEKCRLAHEASGEPYCVGSEGSFGPHPHLPFAASAFEIL
                     AFTDFQRGFSVHEQAIVFETNFASADVGTANELKEFAERAGFPAHALVLRPLDTGGEI
                     AKGIRSWPSLYAAYEHLKELSPTRQVCVETDMRAHMNPTRMRVISSLAKRLARRLSIS
                     CAKCGAPGYGLVDTESGLPCDWCGRPTQMVHREIHGCALCRQRSYLPRHDGLERANPA
                     HCDYCNP"
     gene            complement(3840..>4419)
                     /locus_tag="PLF85_13115"
     CDS             complement(3840..>4419)
                     /locus_tag="PLF85_13115"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF037786.2"
                     /note="catalyzes the formation of 2-oxoglutarate from
                     isocitrate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNE20659.1"
                     /translation="EAELIENAWLTALEDGYRTADIASGRDNEITVGTEAFARAVIDR
                     LGRKPVNLPPAQAFELPPVGPSLEFASAASRALQASVTKELRGIDIFVDWDEADRDPN
                     VLGLKVAAAGSEKLQLRMISNRGVKVFPEGNSATSCADHWRCRFTAESKISHADLLTL
                     QWAIAKAGFDIIKTENLYWFDGKPGYSLGQGE"
BASE COUNT          970 a         1274 c         1204 g          971 t
ORIGIN      
        1 gcgaaccacg aagaacccga agcgctgtaa ggtccccaga tcaggaacat gttggggaaa
       61 cccggcacac tcgctccttc atacgcctgg tagcggttct tctcccagaa gtcaccgagc
      121 tcgataccat cgcgaccgaa catttcaaag gggggcgtat tacctttttc ataaaccttg
      181 aaccctgtgc tcagtaccag tgtatcgatt tcgcgctcgc agccatctgc agtcagaatg
      241 cctgtgggag ttatcctttc tatgcgatcg gtcacgagtt caacattgct cctgttgaac
      301 gtgcgcaaat aatcgttcga aatacttgga cgcttgcagc cgaaaccata gttgggccgg
      361 agtttttcgc gcaccgccgg gtcattgacc tgctcttcga ggtgtttcat gcacagcgcc
      421 tcgagcgcac gaatgagctg cggcgtctgc ttgttgtaga caatacccag catcatgact
      481 atctcggtga agaaggtcgt gacgttgcgc accgactgtt gcgccagtgg taccttggcg
      541 aagatccact gcagccactg cggaatttcg cggtcgggct tcggcagtat ccagatcgga
      601 gtgcgctgga agacgtgcag ctgtttaacg accggcgcaa tcgaaggaac cagctgcacc
      661 gacgttgccc ccgtgccgat caccgccacc cttttgtttt ccagtggata gccgtgatcc
      721 cagcgcgcgg tgtgcattgt cttaccagtg aattcagtta taccaggtat gtcgggcaac
      781 ttgggctgcg tcaggccccc ggtcgcagcg atcaggtatt ttgcccgcca ggttttaccg
      841 tcctttgcct gcacctgcca ggtaccctgc gcttcgttga agactgcttt atccacctca
      901 acgccaaagc ggaatgcgtc gcgtatgccg tacttgtcga cacagtggtg cgcgtacgcg
      961 cgcagctcat ttcccggtgc aaacaagcgc gaccagtgcg ggttctgttc aaaggaaaac
     1021 gaataggtaa acgaggtgat gtcgacggcg attcccgggt aggtgttgtc gcgccaggta
     1081 ccaccgaggt cggcggcctt ttccaggatc acgaaagaga aaccggcctc cctgagcttg
     1141 ataccggcgc caatgccgga aaatccaccg ccgaccacga tcacatcggt atcggtcgcg
     1201 gcattgcctg atgcgccttg cgccacgtct tttgtctcgt acatattcct ccggctatgg
     1261 gtttagcgcc gatcaggcgg gcaagcctga tgtgcgctgc ctatacgcct gaaaaaggat
     1321 aactcccata atctggcgaa atctacaaca ctctgcaaaa gaattcgacc aaacttataa
     1381 aactggacca gcaagctctt gacgacgcga gccggttctg ccagctgcaa actatggcat
     1441 accgcagagt cattggcgcg agcgcgagtg aagacaggct tgaacgcctg attgaagacc
     1501 ggttgaaacc cggcgcagac aaagagaaga tcgacagccg catctgggac ctgttcggtg
     1561 aaacctgggc aatcatgttc accgacctct cgggcttctc gcgcaacgtc gccaagttcg
     1621 gtatcatcca ttttctgcag accatctacg agtcggagcg cctgctgata ccgatcattg
     1681 aagaccacga cggtatctta ctgaaagccg aaggggacag cctgcttgtg attttccgca
     1741 acgccggcaa agcgctgcag gcaggcgtcg ccatgcagcg cagcctcgaa tggtataaca
     1801 aagagcgccc cgaagaagag caggtgctac tctgcgtcgg tatcggttac ggcaaggtat
     1861 tgcgtattgg ggatatcgac gtctttggtc acgaagtcaa cagcgcaagc aagctgggcg
     1921 aagacaccgc gaaggcttat gaaatcctgg taacgcctgc agtgcgcgag gtctttaaag
     1981 actcaggtga atttaggttt gacgcgacag aaggcagcca cttcgcgctg cactggcgcg
     2041 actagcaggc taacaacagc gtccgctcag cgcggttcgc gactttttca ggcattcgtt
     2101 aagagagcag cgcgccgcca tccacaacta tttggctgcc cgtcatataa gaagacatgt
     2161 cggaggcgag aaagagagcc gccttcgcaa tttcgtcggg gtcgcccatg cggtgcatcg
     2221 ggatcttcgc gagaaatgac tccatcacct tcgccatatc gataccctcg ggcatcttgg
     2281 atgcagaact catttctttg acgccctcgg tgacaatgcc gccgggcgct atcgcattca
     2341 cccagatctt gtgctcggca agttcgagcg caacgttctt cgtgaatccc cagacgccgt
     2401 gctttgaggc gtcgtagtgc gccaacccaa cctgcgatgg gtgcagagca tcgatggaag
     2461 tgatgttgat aattctgccg cctttgccct gtgcaatcat ctgcgctgca acttttttgg
     2521 tgcacaggaa aagaccggtg aggtttgtac tgatgacacg ttcaaaatca gccttcgcca
     2581 tctgcatcac gagtttgctc gggaagatac ctgcattatt cacgagtata tcaatgccgc
     2641 cgttttgttt caccgcgtgc gtgaccagct tgtcgatatc ggcctcactg ctcacatcga
     2701 cagtacaacc cgaaacacga cccccctgct ggctgatgag cgcaacagct tcatcgatag
     2761 ccttttggtt aatatcggct atcgtaaccg cagcaccggc ttcagcgagg cgcttcgcaa
     2821 tcgcgcgacc gatgccaagt gcgccgccgg tgacgatggc gctttttccg ctgaggttga
     2881 gaagttcggc tactttttgc atgggcaatt atccccacaa tttctggcct gtaaaccact
     2941 aatgtttctc gattcattgc agatgaaacg tgtgagttat ctaaggatta cagtagtcgc
     3001 agtgcgcggg gttagcgcgc tcgagaccat catgccgcgg cagataactg cgctgccggc
     3061 agagagcaca gccgtgaatt tcccgatgca ccatctgcgt cggtcgcccg caccagtcgc
     3121 acggcagacc gctttctgta tcgaccaggc cataaccagg cgcgccacac ttagcacacg
     3181 aaatcgacag cctgcgcgcc agtcgttttg caaggctgga aatgacgcgc atccgtgtgg
     3241 gattcatgtg agcccgcata tcggtttcga cgcacacctg gcgtgtcggg gagagttcct
     3301 tcaagtgctc gtaggctgca taaagcgatg gccatgaacg aatgcctttg gcaatctctc
     3361 cacccgtatc aagcgggcgc aacacgagcg cgtgcgcagg aaacccggcg cgttcggcaa
     3421 attccttcag ctcgttcgca gtacccacgt ctgctgaagc aaaatttgtt tcgaagacaa
     3481 tcgcctgctc atggacacta aacccacgtt ggaaatcagt aaaggcgaga atttcgaaag
     3541 cagaggcggc gaagggcaaa tgcgggtgag gaccaaaact gccttcgctg ccgacacaat
     3601 agggttctcc actggcctcg tgtgccagac ggcatttttc gagcgcacac tcaagtgttg
     3661 ttcgcgtgcg ctccacctcg ccgctgaaag taccgagcgc atcggtatct gcgcgaaccg
     3721 tacgcaccga aagacccagc tgcctgaacg ctgaccttat ggctcgggct tttgcatgac
     3781 gggtagcgat cactacccgt tcactggtga agagcgacgc tgctggaggc aacgcgccgt
     3841 tattctccct ggcccagcga gtagcctggc ttaccgtcga accaataaag attttcggtt
     3901 ttgatgatgt caaacccggc tttcgcgatc gcccactgca gtgtcagcag atccgcatgc
     3961 gagatctttg attcggcggt gaagcggcag cgccagtggt cggcgcagct cgttgcggag
     4021 ttaccttcag gaaaaacttt cactcccctg ttggaaatca ttcgcagctg cagtttctcg
     4081 ctacccgcag cggcgacctt caaaccgagc acgtttgggt ccctgtctgc ttcatcccag
     4141 tcaacaaaga tgtcgatgcc gcgcagctct ttggtcacag atgcctgcag tgcgcgcgaa
     4201 gccgcagagg caaattcaag cgatggcccg acaggtggaa gctcaaacgc ctgtgccggc
     4261 gggaggttca ccggtttgcg tcccaggcga tcgatcacgg cgcgggcaaa cgcttctgta
     4321 ccgacggtga tttcattatc ccggcccgac gcaatgtccg ctgtacggta gccatcctcg
     4381 agcgccgtca gccatgcatt ttcaatgagc tccgcttct
//
LOCUS       DAOQJF010000278         4410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_11899_length_4410_cov_4.421814, whole genome shotgun sequence.
ACCESSION   DAOQJF010000278 DAOQJF010000000
VERSION     DAOQJF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11899_length_4410_cov_4.421814"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLF85_13120"
     CDS             complement(<1..588)
                     /locus_tag="PLF85_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20660.1"
                     /translation="MLSTKSALPFALLLCLCTATQAQGNTPAVDKTSEPAAPSDIQPE
                     EKVAPVENKPAAVPENTPPDVKAEKAVTPAEAQPATTEPTVPPAIPPQQAEKKTEPAP
                     APVPPAPVENEFRLMTGVHGGLVVPFNGIHSVGYNFGMTLDYTFLRRYGVHFGAETGI
                     LPARAQTLAASPSNISVLDGGTFGFLNLRLTGMYVF"
     gene            632..1183
                     /locus_tag="PLF85_13125"
     CDS             632..1183
                     /locus_tag="PLF85_13125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNE20661.1"
                     /translation="MRVNWKHHRHYIFLDLEATCAKDNGFARSERETIEIGAVALDAG
                     RAVVGEFQEYIRPVHHRTLTAFCTELTGITQRQVDAAAFYPHVFARFSAWVAMFDDAF
                     IFSWGHYDRKQLVQDCRRHEITYDLPKGFADFKNLFFKKQKLLPRQGLELTLSGLGMR
                     FEGRPHSALSDAKNTARLFRYIE"
     gene            complement(1208..1468)
                     /locus_tag="PLF85_13130"
     CDS             complement(1208..1468)
                     /locus_tag="PLF85_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20662.1"
                     /translation="MNKILLIMMIVSTTSFAGSKEKGAQCNMDIECAFGLECSSGVCT
                     KKKEFEFGGAGKSGKKCNIDADCIGSGKCEKNTFGQGVCTGN"
     gene            complement(1550..2329)
                     /locus_tag="PLF85_13135"
     CDS             complement(1550..2329)
                     /locus_tag="PLF85_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNE20663.1"
                     /translation="MKMRPAFLLLAAARLLPAQVLPEGNQTVASFSQARRLVLEIARD
                     NPRTFYCDCVFKDKKIDATSCGYEPARDSVRSRRVEIEHVVPAENFGRSFVEWRKGHD
                     ECIRPGGRPYKGRRCAQKINATYRLMESDLYNLYPEIGELNADRSNFRYGIVEGEERR
                     YGSCDFEIEDRTAEPREKIRGDIARIYFYMDSTYPGRGILGKKSRQLFEAWDQADPVD
                     AQECARARRVEQVQGNANPFTKAPCTAAGLYGNTNVDNGSQ"
     gene            complement(2367..2570)
                     /locus_tag="PLF85_13140"
     CDS             complement(2367..2570)
                     /locus_tag="PLF85_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNE20664.1"
                     /translation="MQKALIIAGILILAAGLVWPWLSKLPIGRLPGDIAIERENMKFY
                     FPLATSLLVSLLLSLVLWLFRRF"
     gene            complement(2577..2975)
                     /locus_tag="PLF85_13145"
     CDS             complement(2577..2975)
                     /locus_tag="PLF85_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE20665.1"
                     /translation="MRLLLDTHTLVWFLTEPKKIPQKTLRLIERPETEVWFSALSLWE
                     LSLKFSLGKLRLKNLSPEDISHEAKEQNFDLITLSETDALGFRNVPRHLHHDPFDRML
                     IWQAIQRDLTLVSAETGLNEYRQLGLRWIW"
     gene            complement(2972..3226)
                     /locus_tag="PLF85_13150"
     CDS             complement(2972..3226)
                     /locus_tag="PLF85_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNE20666.1"
                     /translation="MDALLMVADFKAQFSDVLDKVRQGEEVVLGYGRKKEKIAVLVPY
                     EKYRRTMAGATTRRAGSLAGKGKVIFQDDFKISDDDLLRS"
     gene            complement(3323..4036)
                     /locus_tag="PLF85_13155"
     CDS             complement(3323..4036)
                     /locus_tag="PLF85_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNE20667.1"
                     /translation="MARLIFVDTETTGTEPGKDAICQVCYREGTELFAEYFRPKVPIP
                     VKAMSIHHITNEMVADKPSFAGSAFERDLSERLANGIMVAHNARFDRAMLEAEGVLIK
                     RCICTYRVAYALDTEDTIPEYNLQYLRYYLKLAVEGRAHDAEGDVNVLYALFHHLLGD
                     FMRLRDCTEEAAIAEMEKITTTPLLLRKFNFGKYRGRSISEIAATDRGYLQWLLGQKL
                     DSGENDENWIYTLRHYLGH"
     gene            4227..4299
                     /locus_tag="PLF85_13160"
     tRNA            4227..4299
                     /locus_tag="PLF85_13160"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4260..4262,aa:Lys,seq:ttt)
     gene            4337..4408
                     /locus_tag="PLF85_13165"
     tRNA            4337..4408
                     /locus_tag="PLF85_13165"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4370..4372,aa:Glu,seq:ttc)
BASE COUNT          919 a         1202 c         1256 g         1033 t
ORIGIN      
        1 gaaaacgtac atgccggtga gccgcaggtt taaaaagccg aaggtgccgc cgtcgagcac
       61 ggaaatattg gacggcgagg cggcgagtgt ctgcgcgcga gcgggcagta tacccgtttc
      121 tgcaccgaag tgcaccccgt agcgtcggag aaacgtgtag tcgagcgtca tgccaaagtt
      181 gtagccgacg gagtgaatgc cgttgaacgg taccaccagc ccgccgtgca cgccggtcat
      241 caggcgaaac tcattttcaa ccggcgcagg cggcaccggg gccggggccg gttcagtttt
      301 tttctctgcc tgctgcggcg gaatagccgg cggtaccgtg ggttcagttg tcgccggttg
      361 cgcctcagca ggtgtgactg ccttttccgc cttcacatct ggcggagtat tttcgggtac
      421 ggcggcgggt ttgttttcta caggagctac cttttcttct ggctggatgt cgcttggggc
      481 agcaggttcg gatgttttgt ctactgccgg tgtgttaccc tgcgcctgcg tggcggtgca
      541 caggcagagc agaagggcaa atggcagagc cgatttggtg gacagcatgg cggcaacagt
      601 gcattcgctg tgtaaccgaa aagcgaattc catgcgcgtc aactggaaac accacaggca
      661 ctatattttt ctcgatctcg aagcgacctg cgcgaaagac aacggctttg cgcgcagcga
      721 acgcgaaaca atcgagatcg gcgcggtcgc gctcgatgcg ggccgggcgg ttgtggggga
      781 gttccaggag tatatacgcc cggtgcacca ccggacatta accgcgttct gcaccgagct
      841 caccggcatc acgcagcggc aggttgacgc tgcggccttt tatccgcacg tctttgcccg
      901 cttcagcgcc tgggtggcga tgttcgacga cgcttttatc ttcagttggg ggcattacga
      961 tcgcaagcaa ctcgtgcagg attgtcgccg gcacgagatc acctatgatt tgcctaaggg
     1021 gtttgccgac ttcaagaatc tcttctttaa gaagcagaag ctgctgccgc gccaggggct
     1081 tgaactgacg ctgtcaggtc tcggtatgcg attcgagggc cggccgcact cagcattatc
     1141 tgatgcgaag aacacggcgc ggctcttccg ttacattgag tagggtgcgg agaagggctg
     1201 cctcggctca gttgccggta cagacgccct ggccaaaggt attcttttca cacttgcccg
     1261 agccgatgca gtcggcatcg atattgcatt ttttgccact tttgccggcg cctccaaatt
     1321 cgaactcttt cttcttcgtg cagacgccgc tgctgcattc gagcccgaac gcgcattcaa
     1381 tgtccatgtt gcactgggcg cctttctctt tcgagcctgc gaaggaggtc gtggaaacta
     1441 tcatcatgat aagcagtatt ttattcatag ttctgcaccg gaccacggcc acagagcgcg
     1501 tcaagcctaa gtctgcgcca caccgtgatg caaccgaatc ttgccttctt tattgactgc
     1561 cattatcgac gttggtattg ccgtaaagcc ccgcagccgt acacggcgct ttggtaaatg
     1621 ggttggcatt accctgcacc tgctcaacac gacgagcccg ggcgcattct tgcgcatcaa
     1681 ccgggtcagc ctggtcccat gcctcaaaca gttggcgtga ttttttgccg agtataccgc
     1741 ggcccgggta ggtactgtcc atatagaaat agatgcgggc aatgtcgccg cgaatctttt
     1801 cgcgcggttc ggcggtcctg tcttcgattt cgaaatcgca gctgccataa cggcgctctt
     1861 cgccctcgac aatgccatag cggaagttgc tgcggtcggc attgagttca ccaatctcag
     1921 gatacaggtt gtagaggtca gattccatga ggcggtaggt cgcgttgatc ttttgtgcgc
     1981 agcgccgtcc cttgtagggc ctgccgcccg ggcgaataca ctcgtcgtgg cccttgcgcc
     2041 attcgacgaa gctgcggcca aaattttctg ccggtacgac atgctcgatt tcaacgcgcc
     2101 gtgaccgcac tgaatcacgc gccggctcat aaccacagct ggtggcatcg atcttcttat
     2161 ctttaaagac gcaatcacag tagaaggtgc gcgggttatc acgtgcgatc tcgagaacga
     2221 gtcgccgtgc ctgtgaaaag gaagcaactg tctggttgcc ctcaggtagc acctgcgccg
     2281 gtagtagtcg cgcggctgcc agcagcagaa aggcaggacg catcttcata tctgctgata
     2341 acctgtgcca gattagcctg tcaaattcaa aaccgccgaa aaagccagag caccagcgaa
     2401 aggagcagcg ataccagtag cgatgtcgcc agcggaaagt agaacttcat gttctcgcgt
     2461 tcaatcgcaa tgtcacccgg caggcggccg atgggtagct tgctaagcca gggccataca
     2521 agacctgccg ccaaaatcaa gatacccgcg atgatgagtg ccttctgcat gttcgcttac
     2581 caaatccagc gcaggcccag ctgccggtat tcattcaggc ctgtctctgc gctgaccagc
     2641 gtaagatcgc gctgtatcgc ctgccagatc agcatgcgat caaaaggatc gtgatgcaaa
     2701 tgccggggca cgttgcgaaa ccccagtgca tcggtttcgc tgagtgtgat caggtcaaaa
     2761 ttctgctctt tcgcttcgtg cgagatatct tcgggcgaaa gattcttgag ccgtagtttg
     2821 ccgagtgaaa acttcagcga aagctcccac aggctgaggg ccgagaacca gacttcagtc
     2881 tcaggccgct cgattagccg cagcgtcttt tgtgggatct tcttcggttc tgtgagaaac
     2941 cagacaaggg tatgtgtatc gagcagcagc ctcatgagcg cagtagatcg tcgtcggata
     3001 ttttgaagtc gtcctggaag atgactttgc ccttgccggc gagagagccc gcccgacgtg
     3061 ttgttgcgcc tgccatggtg cgccggtatt tctcgtaggg gacgagcacg gcgatttttt
     3121 cttttttgcg gccgtagcca agcacaacct cttcgccctg ccgtacctta tcgagcacgt
     3181 cagagaactg cgccttgaag tcggccacca ttagtagagc gtccatactt aaagcggggt
     3241 tctaaaagtg acaatatgtc aagtttaaaa tggaatcttg ttttgttgct ttgcgctaac
     3301 aggctccttt ttcaggcgcc tgctagtggc cgaggtagtg ccgcaacgtg tagatccagt
     3361 tttcgtcatt ttcgcccgaa tcaagcttct ggcccagcag ccactgcagg tagccgcggt
     3421 ctgtagcggc aatttcactg atgctgcgac cgcggtattt tccgaagttg aatttcctca
     3481 gtagcagggg agtggtcgtg attttttcca tctcggcgat agcagcttct tcggtgcagt
     3541 cgcgtaaccg catgaaatcc cccagcaggt ggtggaacag tgcatagagc acattcacgt
     3601 caccctcggc atcgtgcgcg cggccctcga cggcgagctt caggtaataa cgcaggtact
     3661 gcaggttgta ttcgggtatc gtatcttcag tatcgagcgc gtaggcaacg cggtaggtgc
     3721 atatgcagcg tttgatcagc actccctcgg cctcgagcat tgcccggtca aaacgcgcat
     3781 tgtgtgccac catgatgccg tttgccagtc gttctgacag gtcgcgctca aaggcactgc
     3841 cggcaaagga gggtttgtct gccaccatct cgttcgtgat gtggtggatg ctcatcgcct
     3901 tgaccggaat cggcactttc ggcctgaaat attcggcgaa cagctcagta ccttcgcggt
     3961 agcagacctg gcaaatggcg tctttgccgg gttcggtgcc ggtagtctcg gtatcgacga
     4021 aaatgagccg tgccatcggg caggcttaac gcgtgcggct tgacgcaaag atttataccg
     4081 gaacagggcc accagtcgcc ggtaaaaatt tgctaaaact tttatcaaat aaattgacgc
     4141 tctgaccaat ttaccggaaa cgccaaggac gctctattga tatgaaacgc aagcggcaca
     4201 gccgattgtt tttcatatga gaaagagaga agctagctca gtcggtagag caactccctt
     4261 ttaaggagtg ggtcccgggt tcgattcccg ggcttctcaa acggtttgct gttcggcagt
     4321 aaacccagaa ggattagtcc ccttcgtcta gcggttagga ctcgggattt tcattcccgc
     4381 aacaggggtt cgattcccct aggggacgca
//
LOCUS       DAOQJF010000279         4351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12145_length_4351_cov_5.313780, whole genome shotgun sequence.
ACCESSION   DAOQJF010000279 DAOQJF010000000
VERSION     DAOQJF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12145_length_4351_cov_5.313780"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2072
                     /locus_tag="PLF85_13170"
     CDS             <1..2072
                     /locus_tag="PLF85_13170"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNE20668.1"
                     /translation="GQMGMNGIVLGMAHRGRLNVLENIMEKPAQYIFAEFKEKADVNT
                     YDNADVKYHLGYSSERVTASGKRVHLTLMFNPSHLEAVDPVCIGSVRARQTLNGDTQR
                     EKYMGILIHGDAAFMGQGVVAETFNLMDIPGYTTGGTLHIVINNQIGFTTTPEESRST
                     SYATDLAKAFHVPVFHVNGDDPEACHRVLTLAMEYKQRYKKDVVIDLICYRRHGHNET
                     DEPSFTQPTMYDIIRKHPTPVEIYEKRLLAEGHTQAALDEIKNRVNESLERSYAETEE
                     KNVTINVDSMKGLWSGFSKSTTEEPATTAEPEELKLVSRAITSFPSGFTPHKKLAQLL
                     ETRAKMAAGHAPLDWGMGEALAFGVLLNRGIAIRLSGQDAERGTFAHRNAVLLDTNTA
                     AKYTPLNNIREGQAQIEVLNSPLSEYSVLGFEYGYSLSSPASLTIWEAQFGDFANGAQ
                     IIIDQFLSSGEVKWNRMSGLVMLLPHGYEGQGPEHSSARLERFLQLCAKENMIVCNLT
                     TPAQIFHVLIRQALRKQKKPLVVMTPKSLLRHPEAISSLGDLVSGRFQEVIGDEKAEA
                     TKVKRVIICTGKVYYDLLKERSARNLEHIALVRLEQIYPFKYDQVGRILEGYPNLSEL
                     FWVQEEPKNMGAWFYIKDRFDERLAGGKFQNMIRCIARKTSPSPAAGLQKVHEREQKE
                     LLDRALG"
     gene            complement(2076..2501)
                     /locus_tag="PLF85_13175"
     CDS             complement(2076..2501)
                     /locus_tag="PLF85_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNE20669.1"
                     /translation="MSDMAMVEESDRLMLDFGKLRKIAAGTADVLPVVVQDAKTLEVL
                     LVAYVNDEALRLSIQEQRAVFYSTSRQEIWRKGETSGDTLSLREIRVNCEQNSLLFMV
                     DKAGSGSCHTRDASGKTRSNCYYRRLKSTELLEFIRASG"
     gene            2595..3026
                     /locus_tag="PLF85_13180"
     CDS             2595..3026
                     /locus_tag="PLF85_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20670.1"
                     /translation="MTSPFDGSELKPATDHVYQDSSGGYLITNPAAVRRRFEAAGRDS
                     NTREVKLENLGVFPLAKDALPLLQKELRHAETTLTILPLLRKSQIPLFSSADAQLVYI
                     PAAGCAICRYFHELWTSDSELSEAADPVLSGFAVLASYAGL"
     gene            3030..3509
                     /locus_tag="PLF85_13185"
     CDS             3030..3509
                     /locus_tag="PLF85_13185"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNE20671.1"
                     /translation="MRSVLFVCLGNICRSPLAQGIFEHQVRQAGIEKDYDADSAGTSG
                     WHEGEPPHGGSIRIARKYGISIERQRSRPVHPKDAEIFDYIIAMDTANRESLLSEFRF
                     PERKVHLMRDFSLSGHQGKNQSVPDPWGMRDDAFENVYAILNDSVAGFITQLREGKI"
     gene            complement(3535..>4351)
                     /locus_tag="PLF85_13190"
     CDS             complement(3535..>4351)
                     /locus_tag="PLF85_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20672.1"
                     /translation="KANPATDYRQNGSTTPVSSGSGSKATEQADGTNYNCFSDVTIIG
                     NCPDVGSELDGNYQVPNVQPGAGGDFANFFIGDQDWVIYEPATNASAFYYNEMWLEGL
                     ASRNSSLQTGPIVGRYYTVTAPELLYFTIVQHWAGDMTQQSHIWATIGYNHGDYEGYA
                     DEKYGKRQVGGSDPDKNYENYVESQGYANSWQNRYSLPVGNIDRVLMEQVFRTYYARL
                     RSGLDTLTSANNSVWTGAATLAVRFSTAEMAIITEKAVLDLRKCRNGASCDNS"
BASE COUNT         1047 a         1210 c         1134 g          960 t
ORIGIN      
        1 cgggacagat gggcatgaat gggattgttc tgggcatggc tcaccgcggg cgacttaacg
       61 tgctcgaaaa catcatggaa aagcccgcgc agtacatctt tgcggagttc aaagagaagg
      121 cagacgttaa tacctatgac aacgccgatg tgaagtacca cctcggttac tcttcagaac
      181 gcgtaacggc ctccggtaaa cgtgtccacc tcacgctgat gtttaacccc agccatctgg
      241 aggcggttga tcctgtctgc attggctctg taagggcgcg tcagacactc aatggggaca
      301 cgcagcgcga aaagtacatg ggaatcctga tccatggcga cgccgccttc atgggccagg
      361 gggtcgtcgc tgagaccttc aacctgatgg atattcccgg ttataccacc ggtggcaccc
      421 tgcacatcgt catcaataac cagatcggtt ttacgacaac gcctgaagag tcacgctcga
      481 ccagctatgc gacagacctc gcgaaagctt tccacgttcc ggtattccat gtcaacgggg
      541 atgatccgga agcctgccac cgtgtgctga cgcttgcaat ggagtacaag cagcgttaca
      601 aaaaagacgt cgtgatcgat ctcatctgct accgccgcca tggccacaac gaaacagacg
      661 agccatcatt cacgcagccg acgatgtatg atatcatccg caagcaccct accccggtag
      721 aaatctacga gaaacgcctg ctcgctgaag ggcataccca ggccgccttg gacgaaatta
      781 agaatcgcgt caacgaaagt cttgaacgca gttatgcgga gacagaagag aagaacgtca
      841 cgatcaatgt cgattcaatg aagggactgt ggtcaggttt cagcaagagc accacagaag
      901 aacccgcaac gacagcagag ccagaagagc tgaaacttgt gtcgcgcgcg atcacttctt
      961 ttccatcagg tttcacaccc cacaagaaac ttgcccagct acttgaaacg agggcaaaaa
     1021 tggctgcagg ccacgcgccg ctggactggg gtatgggcga ggcgctcgct tttggagtgc
     1081 ttctgaaccg tggcattgcc attcgcctca gcggccagga tgcagaacgt ggcacgtttg
     1141 cgcaccgtaa tgccgtgttg ctcgatacga acaccgcagc gaagtacaca cctctcaaca
     1201 atatccgcga aggtcaggcc cagatagagg tactcaattc accgctttca gaatactccg
     1261 ttctggggtt tgaatatggc tattcgcttt cctcacctgc aagcctgacc atctgggaag
     1321 cccagttcgg cgactttgcc aacggggcac aaataatcat agaccagttc ctttccagcg
     1381 gcgaagtgaa atggaaccgc atgagtgggc tggtgatgct gctgcctcac ggctacgagg
     1441 gccagggtcc ggagcactcg agcgccaggc tcgaacgctt tctgcagctg tgtgccaagg
     1501 aaaacatgat tgtctgcaac ctgaccactc cggcacagat ctttcatgtg ctgattcgcc
     1561 aggccctgcg caagcagaaa aagccgttgg tggtgatgac cccgaagagt ctgctgcgcc
     1621 accccgaggc aattagttcc ctgggcgacc ttgtttctgg ccggttccag gaagttattg
     1681 gggatgaaaa agccgaagct accaaggtca agcgcgtaat catctgcacc ggcaaggtct
     1741 attatgacct gctcaaagaa agaagcgcgc gtaatctgga acacatcgcg ctggtccggt
     1801 tggaacagat ttatcctttc aaatacgacc aggtcggcag gattctggaa ggttatccga
     1861 atctcagtga gcttttctgg gtgcaggaag aacccaaaaa tatgggtgcc tggttttaca
     1921 tcaaggatcg tttcgacgag cggctcgccg gtggaaaatt tcagaacatg attaggtgca
     1981 ttgcgcgcaa gaccagcccc agcccggccg cgggtctgca gaaagtgcac gaacgcgaac
     2041 agaaagaatt gctcgaccgc gccctcggct agaaatcagc cggatgcgcg gatgaattcg
     2101 agcaactctg tgcttttcag cctgcggtag tagcagttgc tgcgggtctt gccggatgca
     2161 tcacgcgtgt ggcaggagcc gctccctgcc ttatcgacca tgaagagcag gctgttctgt
     2221 tcgcagttga cgcgtatttc cctgagcgaa agcgtatcgc cgctcgtctc gcccttgcgc
     2281 cagatttcct gcctcgaagt ggaataaaaa accgctctct gctcttggat actcaggcgc
     2341 agagcctcgt cgttgacata ggcaacgagt aatacctcga gcgttttagc atcctggaca
     2401 actacaggaa gtacgtcggc agtaccggcg gcaatcttcc gcaatttacc aaaatccagc
     2461 atgagccgat ccgactcttc gaccattgcc atgtcagaca tgaccccagt cacagactgt
     2521 cggatacccc gtacagcgca cttgctccca tgaaagctct ttacacagca ttccgagtac
     2581 cgtcaggcac gcatatgact tcgcctttcg atggatctga actcaaacct gctactgacc
     2641 acgtctacca ggacagctcc ggtggttacc tgattaccaa tcccgcagct gtgagaagac
     2701 gctttgaagc agccggccga gattccaaca cgcgcgaagt taagctcgaa aacctgggcg
     2761 tatttccgct cgccaaggat gcgctgccac tgctgcaaaa agaactgcgt catgccgaaa
     2821 caacactcac tatccttcct ttgctcagga aaagccagat tccactgttt tcttcagccg
     2881 atgcccaact cgtctatatt cctgcagcgg gatgcgccat ctgccgctac ttccatgaac
     2941 tctggaccag cgacagcgag ttgagtgagg ccgctgatcc ggtgctttcc ggttttgctg
     3001 tactggcatc gtacgcaggc ttatagcgca tgcgcagcgt gctttttgtc tgcctgggca
     3061 atatctgtcg gtctccgctg gcgcagggca tttttgaaca ccaggttcgc caggcaggta
     3121 tagaaaaaga ttacgacgcc gattccgcgg gaacttcggg ctggcatgaa ggtgaaccac
     3181 cacacggtgg ctcaatacgc atcgccagga aatacggtat cagtattgaa agacagcgca
     3241 gccggccggt gcatccaaag gacgccgaaa tttttgatta cattatcgcg atggatacgg
     3301 caaaccggga atccctgctt tcagaattcc gctttccgga acgtaaggtg cacctgatgc
     3361 gcgacttcag cctgagcggg catcagggca aaaaccagtc cgtacccgat ccatggggaa
     3421 tgcgcgacga cgctttcgag aatgtatatg caattctcaa cgattccgtc gcgggtttta
     3481 taacgcagct acgggaaggg aaaatctagc catctcgggt cagccaaggc cgaactaact
     3541 gttatcgcag ctggcgccgt tccggcattt ccgcaggtcc aatacagctt tttctgtaat
     3601 gatcgccatt tcggctgttg agaagcgaac agcaagtgtt gctgcccccg tccaaaccga
     3661 gttgttagcg cttgtgagcg tatcgagtcc cgagcgcaga cgcgcataat acgtacggaa
     3721 aacctgttcc atcaagacac ggtcaatatt gcccactggc aggctatagc ggttctgcca
     3781 ggagttggcg tacccctggc tttcgacgta attttcatag ttcttgtccg ggtcgctgcc
     3841 gccgacctgc ctcttgccat acttttcatc ggcataacct tcgtaatcac cgtggttgta
     3901 acctatcgtt gcccagatat gtgactgctg cgtcatatca ccggcccagt gctggacaat
     3961 agtaaaatac aggagttcgg gcgccgtgac agtatagtac cgaccgacaa tagggcccgt
     4021 ctgcagggaa ctgtttcgtg atgccaggcc ctctagccac atttcattgt agtaaaatgc
     4081 tgatgcattc gttgcaggct catagatcac ccagtcctgg tcgccgataa agaagttagc
     4141 aaaatctcct ccggcaccgg gctgtacatt aggcacctgg taatttccat ccaattctga
     4201 cccgacatca gggcaattac cgattattgt aacatcgcta aagcaattgt aatttgtacc
     4261 atccgcctgt tcggtagcct tgcttccgct acccgacgaa acaggcgtgg ttgaaccgtt
     4321 ctgcctgtaa tcggttgcgg gattagcctt g
//
LOCUS       DAOQJF010000280         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12154_length_4350_cov_5.524331, whole genome shotgun sequence.
ACCESSION   DAOQJF010000280 DAOQJF010000000
VERSION     DAOQJF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12154_length_4350_cov_5.524331"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLF85_13195"
     CDS             complement(<1..538)
                     /locus_tag="PLF85_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE20673.1"
                     /translation="MPVTVQGDLLEMKITPVDRALRIKALREKQKVERDRKVRERAER
                     IAGKRGDGTKDKKEKPPRRARGSLSREEIVQAALDIIRHDGVEGLSMRRIAERLGCSV
                     ASPYAHFENQEEILRLLITDGEKILTADLKAAQASSDDVYSQLSAIAHAYWNFASENR
                     QLHRLMFSAAGGKLYRRSF"
     gene            complement(590..1405)
                     /locus_tag="PLF85_13200"
     CDS             complement(590..1405)
                     /locus_tag="PLF85_13200"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNE20674.1"
                     /translation="MATILIFFAAHWLASAFAQSFFLHRYASHSMFTLSKGWEKFWHL
                     FTIIAQGPSYLNPRGYAILHRMHHQFSDTEKDPHSPHHAKNFFDMMLKTKHEYDSYAY
                     GKVEAPKAFSENTPSWPAVDNLGKSWVFRIGAGALYAFFYMAFVPEGMWYLYLLLPLH
                     WLMGPIHGALVNWGGHMYGYTNYKLKDESRNALPIDFLVGGELYQNNHHALGNRPNFA
                     KRWFELDTTYQVMRVLDLLHIIRIKHPAAEPQKSESKAKSASKASAGRRPVAA"
     gene            1685..2434
                     /locus_tag="PLF85_13205"
     CDS             1685..2434
                     /locus_tag="PLF85_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE20675.1"
                     /translation="MKVGLMGFGKTGKAVATTILRHADFRLEWVLRRSHKLEHRSIPE
                     FLGEESDNPGLIHSIDEMPVGELLDRQPVEAIIDFSSAEGYRYYGEEAARRCIKIISA
                     VSHYPAATLKVLRSLSRKTPVFWSPNITIGVNYLIMAARFLRKIAPGIDMVVLEEHFR
                     DKAGVSGTARVIAENLEIPAEEIKSVRAGGIIGRHEIVCGFPFQTVRLTHESIAREAF
                     GNGAIFVAQNLQNKAPGFYNFEDLLTPYFAL"
     gene            complement(2439..2609)
                     /locus_tag="PLF85_13210"
     CDS             complement(2439..2609)
                     /locus_tag="PLF85_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20676.1"
                     /translation="MKLVQNADKQTFTATELSFGEVKAIRDALKAFAKGGSAQAAKLA
                     GEIEAALDNMQV"
     gene            2799..3263
                     /locus_tag="PLF85_13215"
     CDS             2799..3263
                     /locus_tag="PLF85_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE20677.1"
                     /translation="MATKFKGTGAETRALDLFIKLMRTHDALSAHLRALIAGFDLQPS
                     QFGILETLYHLGPMQQHELAAKLLQTPGNITRILDKLEQEGLVERGSEPGDRRCHRIA
                     LTQQGEKRISTIFPKFAAAMDELFKDLTADEQKQLNDLLRKTGKSLAYSLNK"
     gene            3282..3665
                     /locus_tag="PLF85_13220"
     CDS             3282..3665
                     /locus_tag="PLF85_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE20678.1"
                     /translation="MNSIIDSVSKSGRYIFTIPLIMFGINHLTMADLMAGMVPVPGGA
                     FWVYFTGVAMLAAAVAIITNFKGLGSLAAFLAGVLILVYVLAIHLPGVINAKDAMARM
                     TPMMATLKDLGMVGGAWAIARLMKA"
     gene            3668..3931
                     /locus_tag="PLF85_13225"
     CDS             3668..3931
                     /locus_tag="PLF85_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNE20679.1"
                     /translation="MAAHIAAKQPAQVELEAGKSYYWCSCGHSKNQPFCDGSHNAVNK
                     ALPEGESKFVPLKFEAEKSGTHWLCQCKQTGKAPFCDGSHKSL"
     gene            3986..>4350
                     /locus_tag="PLF85_13230"
     CDS             3986..>4350
                     /locus_tag="PLF85_13230"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNE20680.1"
                     /translation="METALQHINDIAASIGIPAEALTLYGQHKAKVPYSLYDAAQAKQ
                     AKLILVTSTTPNKAGVGKTTTSVALGQGMRRLGKKAVVALREPSLGPVFGMKGGATGG
                     GAAQVHPTADINLHFNGDF"
BASE COUNT          990 a         1200 c         1212 g          948 t
ORIGIN      
        1 gaaacgacct gcggtagagt ttaccgcccg ccgcgctgaa catcaaccgg tgcaactggc
       61 ggttttcgct cgcgaagttc cagtaggcgt gcgcgatggc actgagctgc gagtacacat
      121 cgtcggagct tgcctgtgct gctttcaggt cggcagtgag tattttctcg ccgtcggtaa
      181 tcagcaggcg cagtatttct tcctggtttt caaagtgagc atagggactg gccacagagc
      241 agccgagccg ctcggcgata cgccgcatgc ttaagccttc gacgccgtcg tggcgaataa
      301 tgtcgagcgc cgcctggacg atttcttcgc gactgagcga accgcgggcg cgccgcgggg
      361 gtttttcttt cttgtctttg gtaccatcgc cgcgtttgcc ggcgatgcgt tcggcgcgtt
      421 cgcgtacctt gcggtcacgc tccacctttt gtttttcgcg caacgccttg atgcgcagcg
      481 cccggtcaac cggggtgatc ttcatttcga gaagatcacc ctgtacagtc accggcacgt
      541 aaactccaaa agcggcgggc gcggagactt tccggaatct gccaagatct tacgccgcaa
      601 ccgggcgacg accggctgat gctttcgacg ccgacttggc ttttgactca gatttttgcg
      661 gctctgctgc cgggtgcttg atacgaatga tgtgcagcag gtcgagtacg cgcatcacct
      721 gataagtagt atcgagctcg aaccagcgct tcgcaaagtt cgggcggttg cccagcgcat
      781 ggtggttatt ctggtagagt tctccgccca cgagaaagtc gatcggcagg gcattgcgcg
      841 actcgtcttt cagtttgtaa ttggtataac cgtacatgtg accgccccag ttgaccagtg
      901 cgccgtgaat tggccccatc agccaatgca ggggcagcag caggtacagg taccacatac
      961 cttcgggtac aaatgccatg taaaagaatg cgtagagcgc acctgcgcct atgcggaaca
     1021 cccatgactt gccgaggtta tcgactgccg gccatgacgg tgtgttttca gagaaggcct
     1081 ttggcgcttc aaccttgccg tatgcatagc tgtcatattc atgcttcgtt ttcagcatca
     1141 tgtcgaagaa attcttcgcg tggtgcgggc tgtgcgggtc tttttcggta tctgaaaact
     1201 ggtggtgcat cctgtgcaga atagcatatc cacgcgggtt caggtacgat gggccctgcg
     1261 cgataatcgt gaacaggtgc cagaatttct cccagccctt gctcagggtg aacatgctat
     1321 gcgaagcata gcggtgcaga aagaagctct gcgcgaaagc ggacgccagc cagtgggccg
     1381 caaagaaaat gagtatcgtt gccatagtac ccttttgatg agggggagct caatcgggtt
     1441 gcaaaaaaat ctaacagtgt tcgttataga tccgtttttt caggctggcg gctggccgca
     1501 gaccgtctat ggcatgtttt aggggaaatt ccccggcttt ttagccatga ttccgttggt
     1561 agttactaaa gaccgccgga aaagggggct tggaagccct ttcaggcacg gtagaacgat
     1621 caaaatagga tataaatccc atattaatga cttttcatac cgttccggga tgccagtagg
     1681 gcagatgaaa gtagggctga tgggttttgg caagacgggc aaggctgtag ccacgaccat
     1741 tctgcgccac gcggattttc gtctcgaatg ggtactgcgc cgctcgcaca aactggagca
     1801 ccgttcgata cccgagtttc tcggcgaaga gtcagataac ccggggctga tccactccat
     1861 cgacgaaatg cccgtgggcg agctgctcga ccggcaaccg gttgaggcaa ttatcgactt
     1921 ctcgtctgct gaaggctacc gctactacgg cgaagaggcc gcccgccggt gcatcaagat
     1981 cattagcgcg gtgtcacact acccggccgc gacactcaag gtgctccgca gcctttccag
     2041 gaaaaccccg gtattctggt caccgaacat cacgatcggc gtcaattacc tgatcatggc
     2101 agcacgtttt ttgcgcaaaa ttgccccggg aattgacatg gtagtgctgg aagagcactt
     2161 tcgcgacaag gccggggttt ctggtacggc acgggtaatt gcggagaacc tggaaatacc
     2221 tgccgaagag atcaaatcgg tacgcgcagg cggcattatt ggtcgccacg aaattgtctg
     2281 tggatttccg ttccagaccg tgcgactaac gcacgagtct attgcccgcg aggcatttgg
     2341 caacggggca atctttgtgg cgcagaacct gcagaacaag gcgccaggct tctacaactt
     2401 cgaagatctg ctcacgccct actttgcact ctgatctctc agacctgcat attgtcgagc
     2461 gccgcctcga tctcacccgc cagtttcgcg gcctgcgctg agccgccttt ggcaaaagcc
     2521 ttcagcgcat cgcggattgc cttcacttcg ccaaacgaaa gttcggtcgc ggtgaaggtt
     2581 tgtttgtcgg cgttctgtac cagtttcatg cctgtgcctg gcataggtac tcgtgacggc
     2641 aagcggttca gctcgacctg gttacttcgg tcaattgtga caaaactcct ggcatagcca
     2701 gcctgtctaa cggtgtaccc gtcccgagac agcaaaaacc tctcaaaaaa ttattgccga
     2761 cacaatagtt gtattgtaaa ctattgtcaa agcaactaat ggcgacaaag ttcaaaggta
     2821 ctggggcaga aacaagagcg cttgatctct tcatcaagct gatgcgtacg cacgacgcgc
     2881 tgtcggccca cctgcgcgcg ctgattgccg gttttgacct gcaacccagc cagtttggca
     2941 ttctcgagac gctctaccac ctgggcccca tgcagcaaca tgaactggca gccaagctgc
     3001 tacagacgcc aggaaacatt acccgcatcc tcgacaagct ggagcaggaa ggcctggttg
     3061 aacgcggctc cgagcctggt gaccggcgct gccatcgtat tgcgcttacc cagcaagggg
     3121 aaaagcgcat cagtaccatt ttccctaaat ttgccgcggc catggacgaa ttgtttaaag
     3181 acctcacggc agacgagcag aagcagctga atgacctctt gcgcaagacc ggcaagagcc
     3241 tcgcgtattc attaaataag tgaataaaaa aggagaataa aatgaattca ataatcgaca
     3301 gcgtaagcaa aagtggcagg tacatcttca cgatacctct gatcatgttc gggataaacc
     3361 acctgaccat ggcagacctg atggcgggca tggtacccgt accgggcggc gcgttctggg
     3421 tatatttcac tggtgtagcg atgctggccg cagctgtagc gatcatcacc aactttaaag
     3481 gcctgggttc cctggcagcc tttcttgccg gtgtactgat tctcgtctac gtgctggcaa
     3541 tccacctgcc gggcgtgatc aacgcgaaag atgcaatggc acgcatgacg ccgatgatgg
     3601 caacgctcaa agacctcggt atggttggcg gcgcatgggc gattgcgcgg ctgatgaagg
     3661 cataacgatg gctgcccata tcgctgcaaa acaaccagcg caggttgagc tcgaagccgg
     3721 caagagctac tactggtgct cttgcggtca ttcgaagaac cagccctttt gcgatgggtc
     3781 gcacaacgcg gtgaataaag cactgcccga aggggaaagc aagttcgtac ccctgaagtt
     3841 cgaagcagaa aaatccggaa cgcactggct gtgccagtgc aagcagaccg gtaaagcacc
     3901 gttctgcgac ggatcgcata agagtttgtg aaaaaattgg ctgcgcgctg acgcgcgcag
     3961 ccatcttggc ggtagacctg tccccatgga aaccgcactc caacacatca acgatatagc
     4021 cgccagtata ggcataccgg cagaagcgct cacactctac ggccagcaca aggccaaggt
     4081 gccctactcg ctctacgacg ccgctcaggc gaaacaagca aagctcattc tcgtcacttc
     4141 gactacgccc aataaagcgg gcgttggtaa gaccactacc tcggtggcac taggccaggg
     4201 catgcgccgc ctcggcaaaa aagccgtcgt cgccttgcgt gagccttcac tcggaccagt
     4261 gttcggtatg aaaggtggtg cgacgggtgg cggcgcggca caggtgcacc cgacagccga
     4321 catcaacctg catttcaacg gcgacttcca
//
LOCUS       DAOQJF010000281         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12231_length_4330_cov_4.750409, whole genome shotgun sequence.
ACCESSION   DAOQJF010000281 DAOQJF010000000
VERSION     DAOQJF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12231_length_4330_cov_4.750409"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLF85_13235"
     CDS             complement(<1..519)
                     /locus_tag="PLF85_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE20681.1"
                     /translation="MKAHVLENSFGIENIHLRERELADLDSHEIRIAMKAASLNFRDY
                     LMVTGNYNPKLRLPMVPLSDGAGEVVAVGKSVTRFKVGDRVMPTFSQNWIAKRPVSDD
                     LRKSTLGGPLDGTACEIMQLPEWAAVEIPEGLSYAEAATLPCAALTAWSSLVTMNQAK
                     PGEYVVVPGTGGV"
     gene            complement(525..1220)
                     /locus_tag="PLF85_13240"
     CDS             complement(525..1220)
                     /locus_tag="PLF85_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20682.1"
                     /translation="MYIYFIAAFSLAALACWGLSSILRKRGRNLPLIFFGGLAYILVA
                     LPIVWSLYLYFALGFIGIGVLFMALSTYSLPAKLQAPLVALPIVSLYALMWYNEPSYN
                     IFLIPEGYRGRVVIVHGCKDGAPREFEGFYRIYRIGQNGLLKSRFSFAGSAFDSLHSK
                     YFFVNKFGKRSPISEDISPQSVAIQGLWTLGAERNGETGIDFIVDAPITDTEAYRQNE
                     KENWQREIDSCAR"
     gene            1327..3066
                     /locus_tag="PLF85_13245"
     CDS             1327..3066
                     /locus_tag="PLF85_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE20683.1"
                     /translation="MAAKTLIQNGLVYDGTGNKPTESDVLIENGKIAAIAPHGTIKAS
                     NAKKINAQGMWVTPGFLDTHTHYDAELALSPGLTESVRHGVTSVVVGSCSVSFVSSSP
                     EDCADMFTRVEAVPREVVLPLLKKIKKWDSPAGYRKWIDKHPLGPNVASFMGHSDLRA
                     RAMGLHRSVTTERPTSAEQKQMEATLAEALNAGFLGMSCMTNPWDRLDGDREWSASLP
                     SYYARRAEKKPLLEMLRRRHRIHQSAPNLVTRVNVLGMALASGGLARESLRTTVITMM
                     DLKADPYVLGLTNFVAWLANKVFRGNFRWQSPPVPFDLYYDGWDSVLFEEFYAGQKIR
                     DAAKDLNSRHALLSDPTFRKRFKKDLLRKLAPKVWHRDLADTTILSCPDKSAVGKSFA
                     QVAAERGQDAIDTFLDLLLKYDRDIRWHTCLANHRPEKVAEIMKNPATLMSFADSGAH
                     LRNMAFYNFPLRMLKQVRDAELAGKAIMPVEKAVWRLTGELAQWFGLDAGALKPGAQA
                     DVVVIDPKRLDDSLEKVKLQPWSKGFKFDRIVNEGAAARHVFIAGQAAIINGRPAPAL
                     GKKRMGRFLESVL"
     gene            3178..3414
                     /locus_tag="PLF85_13250"
     CDS             3178..3414
                     /locus_tag="PLF85_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20684.1"
                     /translation="MQAMSLKVEDEILKDAEHFVKRRKISRNAYINEAIKAYNALERR
                     RELREKLAVESKLVAADSLAALKELEMIADFHEN"
     gene            3404..3745
                     /locus_tag="PLF85_13255"
     CDS             3404..3745
                     /locus_tag="PLF85_13255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNE20685.1"
                     /translation="MRIEKFGIYIADLNPRYGTEPGKKRPVVVVQTDLLNGVHPSTIV
                     CPISTKVAPKAEILRVHIDLKPAGLKRASDILVDQVRSIDNRRFVEQRGVLSAKNQRQ
                     LLESLKILLLE"
     gene            3818..3946
                     /locus_tag="PLF85_13260"
     CDS             3818..3946
                     /locus_tag="PLF85_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20686.1"
                     /translation="MLDYLLAGGILAVAALYLLRYYLPGRKQVGCAKCSAAGPVKS"
     gene            3979..4242
                     /locus_tag="PLF85_13265"
     CDS             3979..4242
                     /locus_tag="PLF85_13265"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNE20687.1"
                     /translation="MKKTKTSTAKKKAPQPTGRNLANLAPGEGGVILATPLHHERLAE
                     IGLVKGERVEVMKVAPFGDPVILQVLDAPVIVRRADLEGVLVL"
BASE COUNT          986 a         1209 c         1225 g          910 t
ORIGIN      
        1 aacgccgccg gtgccgggga ccaccacgta ttcgcctggc ttcgcctggt tcatggtcac
       61 gagcgacgac caggcagtga gggcggcgca cggtagagtc gcggcttcgg catagctcaa
      121 accttcggga atttcgaccg ccgcccattc gggtagctgc attatttcac aggcagtgcc
      181 gtcgagtggg ccgccgagtg tgctcttacg cagatcgtcg ctcaccgggc gtttcgcaat
      241 ccagttctgc gagaaggtcg gcatgacacg gtcgccgacc ttgaagcggg tcactgactt
      301 gccgacggcc acgacttcac ccgcgccgtc agaaagcggc accatcggca gcctgagctt
      361 cgggttatag ttgcccgtca ccatcaggta gtcacgaaag ttgagcgacg ccgctttcat
      421 cgcgatgcgt atctcgtgcg aatctaaatc cgccagttcg cgctcgcgca ggtgaatgtt
      481 ctcaatgccg aaactgtttt caagtacgtg ggctttcatg ttattcatct cgcacagctg
      541 tcgatttcgc gctgccagtt ctccttttca ttttgcctat atgcttcagt gtccgtgatg
      601 ggtgcgtcga caataaaatc aattcccgtt tcaccattgc gttcggcacc cagagtccac
      661 agaccctgta tcgccaccga ctgcggagaa atatcttcgg aaatcgggct gcgtttgccg
      721 aacttgttga caaaaaaata tttcgagtgc aggctgtcga aggcgctgcc ggcaaaagaa
      781 aatcggctct tcaacaggcc gttctggcca attctataga tgcggtagaa cccttcgaac
      841 tcacgtggcg ctccgtcttt gcagccatgg acaatcacaa cacggccacg atagccttcg
      901 ggtatgagga atatattgta agaaggttca ttgtaccaca tcagggcgta caggctgacg
      961 atgggcagcg ccacgagcgg agcctgcagc tttgccggca gagaatatgt gctgagcgcc
     1021 ataaagagca cgccgatgcc gataaacccg agcgcaaagt acagatacaa cgaccaaacg
     1081 atcggcaagg cgaccagaat ataagcgagc ccgccgaaaa aaatcagcgg cagattgcgc
     1141 ccgcgcttac gcagaatcga tgacagaccc cagcacgcaa gagcggcgag tgaaaatgcg
     1201 gcaatgaaat agatgtacat gcaagaatcc tggcgtctga cgacgctgcg tcgatgggta
     1261 atgccttggg ctgccgtaaa gaacctttgc ggttgacgcc gaacacactt tggcggtcag
     1321 agagctatgg ccgccaaaac acttatccag aatgggctcg tctacgatgg caccggaaac
     1381 aaaccgacgg aatctgatgt actcatcgaa aatggcaaga tcgccgcgat cgcgccgcac
     1441 ggcaccatca aggcctcaaa cgccaaaaag atcaatgcgc agggcatgtg ggtcacaccc
     1501 ggctttctcg acacgcacac gcattatgat gcggaactcg cgctgtcgcc ggggcttacc
     1561 gaatcggtgc gtcatggtgt gacgagtgtt gtcgtcggca gctgctcggt gagctttgtg
     1621 tcatcgtcgc ccgaagattg cgccgacatg ttcacgcgcg tcgaggctgt gccacgcgaa
     1681 gttgttctgc ctctattaaa gaagataaag aaatgggatt cacccgcagg ttaccgcaaa
     1741 tggatcgata aacatccgtt aggccctaat gtcgcgagct tcatgggcca cagcgatctg
     1801 cgagcgcggg cgatgggact gcaccgctcc gtcacgaccg agcgccccac gtctgcagaa
     1861 cagaagcaga tggaagcaac gctcgccgaa gcgctcaatg ccggttttct gggcatgagc
     1921 tgcatgacca acccatggga tcgcctcgac ggcgaccgcg agtggtctgc ttcgctgccg
     1981 agctattatg cccgccgcgc ggagaagaaa ccgttgctcg agatgctgcg ccgtcgccac
     2041 cgcatccatc aatccgcccc taacctcgtc acacgcgtca acgtgctcgg catggcgctt
     2101 gcgagcggtg gtttggcgcg cgagagcctg cggactacgg tgatcacgat gatggatctc
     2161 aaggccgacc cgtacgtgct cggcctgacc aactttgtgg cctggctggc gaacaaagtc
     2221 tttcgtggca actttcgctg gcaatcgccg ccggtaccgt tcgatctcta ttacgacggc
     2281 tgggattcag tactcttcga agaattctac gcagggcaga aaatccgcga cgcggcgaaa
     2341 gatctgaatt cgcgccacgc gctgctttcc gatccaacat tccgcaagcg gttcaagaaa
     2401 gacctcttgc gcaagctcgc gccgaaagtc tggcaccgcg atctcgccga taccacgatc
     2461 ctgtcatgcc ccgataaatc tgccgtcggc aaaagtttcg ctcaggtagc agccgagcgc
     2521 gggcaggatg caatcgatac ctttctcgac ctgctgctga agtatgaccg cgacattcgc
     2581 tggcatacgt gcctggcgaa ccatcggcct gagaaggtcg cggagattat gaagaacccg
     2641 gcgacgctga tgagctttgc cgattcgggc gcgcatctgc gcaacatggc attctacaac
     2701 tttccgctgc gcatgctgaa gcaggtgcgc gacgccgaac ttgcgggcaa agctattatg
     2761 ccggtcgaga aagcggtctg gcgtctcacc ggcgaactgg ctcagtggtt tggtctcgat
     2821 gcgggagctc tgaagccagg cgcgcaggca gacgtggtcg tgattgaccc gaagcgtctc
     2881 gacgactctc tcgaaaaggt aaagcttcaa ccctggagca aaggtttcaa attcgaccgc
     2941 atcgtgaacg aaggcgccgc cgcgaggcat gtgttcattg cggggcaggc cgcgataata
     3001 aacggtaggc cagcccctgc gctcggtaag aagcgcatgg gtaggttttt ggagagtgtg
     3061 ctgtgagcct gcccgttttt gccggatgtt tgatgatccc gcttgagaat attccattct
     3121 gcaaattttc ttgacggcca atcaagatac cgctttagta tcactaaagg tgataccatg
     3181 caggccatgt ccctaaaagt cgaagatgag atccttaaag acgcagaaca tttcgtgaaa
     3241 cggcgcaaga tttcgcgcaa tgcttacatt aacgaggcca ttaaggccta taatgcactg
     3301 gagcgtaggc gtgagctgag agagaagctg gcggttgagt caaaactcgt tgccgcagat
     3361 tctctcgctg cattgaaaga gctagagatg atagccgact ttcatgagaa ttgaaaagtt
     3421 tggtatctat atcgctgacc tgaatccacg ttacggcacc gagccgggca agaagcggcc
     3481 tgttgttgtt gtgcagaccg acctgctgaa cggggtacat cccagcacta ttgtttgccc
     3541 gatttcgact aaagtagctc ccaaagcaga gatactgcgc gtgcatatcg acctgaagcc
     3601 tgccgggttg aagcgcgctt cagacattct ggtcgatcag gtgcgctcga tcgacaatcg
     3661 ccgtttcgtc gagcaacgcg gtgtgctgtc ggcgaagaac cagaggcaac tgctcgagtc
     3721 attgaagatt ctgttgctgg agtaggatca ccccaaactc aattgacaat cattctcaat
     3781 tggcttgacg gcgggtcggt aaacgcgggc tgaaagtatg ctcgattacc tgcttgccgg
     3841 tggcattctc gcggttgccg cactctacct gttgcgctat tacctgccgg gccgcaagca
     3901 ggtcggctgc gccaaatgtt cagcagcggg ccctgtcaaa tcatagtctg ttcgctgcat
     3961 ttatccaggt atcccatttt gaaaaaaaca aaaacctcca ccgccaaaaa aaaagccccg
     4021 caaccgactg gccgcaacct tgcgaacctt gcaccgggcg aagggggagt aattctcgcc
     4081 acgccgctac accacgaaag actggctgag atcggcctcg tgaagggtga acgcgtcgag
     4141 gtcatgaaag tggcgccgtt cggtgacccg gtgattctgc aggtgctcga tgcgcctgtg
     4201 atcgtgcgcc gcgccgacct ggaaggagtg ctcgttttat gacagcacct gtcactcagg
     4261 cctttcgtgt tgcgctgatc ggcaacccga acgcgggtaa gacgacggtc tttaatgctc
     4321 tgtgcggtgc
//
LOCUS       DAOQJF010000282         4322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12261_length_4322_cov_4.147176, whole genome shotgun sequence.
ACCESSION   DAOQJF010000282 DAOQJF010000000
VERSION     DAOQJF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12261_length_4322_cov_4.147176"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..950
                     /locus_tag="PLF85_13270"
     CDS             162..950
                     /locus_tag="PLF85_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNE20688.1"
                     /translation="MTLETVAGSLAPNRFQVEFHTRAGSEQKGPLAVLWGLIESYEVD
                     VFSVSLSRITADFLHYMSTAQISLDEQADFTQTAARLLFYKSRLLLPNAVIGEETPPD
                     KLPYELVEQLLEYKKMQQAAEEMRRLEERSQLRLTREPQWSAFESDSDYLEVDLLSFL
                     RTFRDFLEREEKSRPMQIQDETISAEEMMEYMMSRLMGDTEASFFRSVTGFSVARIIA
                     CFLALLELAKQKMVVLRQDEWLGDIHFAPAPSYDGQTELPFGAT"
     gene            947..1486
                     /gene="scpB"
                     /locus_tag="PLF85_13275"
     CDS             947..1486
                     /gene="scpB"
                     /locus_tag="PLF85_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNE20689.1"
                     /translation="MSDTAYLKAAIEGLLFISGNGLTAAEIANGLNENAEQVKIALDS
                     LLDDYAERESGIAIQEISGRYQFTTQPNIYPAIQNFLRQKKKETLSKSTLETLAIITY
                     KQPITLPEIEEIRSVSSRAQLIALQQKKLVKAMGHKETVGKPTLYGTTKDFLRYFGLN
                     SLDELPPPQDVKELNFDEL"
     gene            1526..2602
                     /gene="pheA"
                     /locus_tag="PLF85_13280"
     CDS             1526..2602
                     /gene="pheA"
                     /locus_tag="PLF85_13280"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNE20690.1"
                     /translation="MKQYRDQIDRIDADIVRLIGQRAEIAREIAAAKAKDNLPLYQPD
                     REQQVYDKIARLNPGILDDESLRNIYREIMSATLKLEGSIQVGYFGAEGSFTHQAALR
                     KFGRSLSLAPFRTIDDVFQAVQRREIKYGVVPIENSTEGMVKATLDALVKFDLNIYSD
                     IVLQIRQSLLTRATELSQIEKIYTHPQAYAQARNFIMKNLAQAEWIETASTSEAVRLV
                     AQENSERLAAIASDVAGEIYGLRPLVEDITDYKRNFTRFIVIGHDVAKRAERNRTMIS
                     FNLPDTTGSLYRALAPLYEQKVNMRAIESRPDRNQVWSYIFFVDLEGHQEDEAMKRAF
                     ELMRPLTSGFRVLGSYPVEKGPED"
     gene            complement(2603..3472)
                     /locus_tag="PLF85_13285"
     CDS             complement(2603..3472)
                     /locus_tag="PLF85_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20691.1"
                     /translation="MKDIAVVYKYSALESYSPRQIRRHRELNPELTQRMQSAHEEHAG
                     ALESLRRLIAQLGAGASWFERDTLNRSLDDFRLVISFGGDGTFINSSHFIEKTLLLGI
                     NSSPDNSVGHYCRYELRSKQGETRLRRDLLALLESSAKRPAESQLMRLKVTLQDRPAP
                     FPVLNDVLFSEQNPAATSRYTLRWRNRVHHQKSSGIWVTTPTGSTAAYASAGGKPFTR
                     RELRFIVRELYSDAAPRRLTHGTVRAGETLKVVSSLMHGALWLDGSHHRMPVALGEHI
                     TIGIHPQRLRAIY"
     gene            complement(3513..>4322)
                     /locus_tag="PLF85_13290"
     CDS             complement(3513..>4322)
                     /locus_tag="PLF85_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNE20692.1"
                     /translation="EKSLYQLSRTVETFSGKRLAANAPIVGEDVFTQTAGIHADGDKK
                     GGLYESRLLPARFGRDRRYALGKLAGKASLDQNLKTLGISLADEERAQVLSRIIELGD
                     QKKQVTIDDLPFIIGEVLKKPEEKILEITQVTVTSGMHIKPMCSLTVEYRGKAVQVSG
                     QGEGGYDAFMSALRSWAKEHKINLPRLTDYEVRIPPGGQTSALVECKITWNIAGLAAA
                     KEKTDSDKAMFGGELTVGRDSNILTTRGVDPDQVIAAIRATERMLNVILRK"
BASE COUNT          978 a         1276 c         1198 g          870 t
ORIGIN      
        1 gccaagctcg aaccattccg tgccaagcgt gcagaacttg cagcaaagcc cggacaagtg
       61 ggcgaaattc ttgccgcggg agccgataag gcccgtggcg tagcgcgcaa caccatggaa
      121 cgcgtgaagg aatccatggg cctcgtttcg aggtacacac tgtgacgctc gaaactgtag
      181 caggttctct tgcgcctaac cgtttccagg tagaatttca cacgcgcgcg ggttctgaac
      241 aaaagggacc gctggccgta ctctggggac tgatcgaaag ctacgaggta gacgtattct
      301 ccgtttcact ttcgcgtatc acggctgatt ttcttcatta catgagcacc gcgcagattt
      361 cgctcgacga gcaggcagat ttcacccaga ctgcagcccg cctgctcttc tacaaatcac
      421 gcctgctgct gccgaatgca gtgattggcg aagaaactcc gcccgataaa ctaccatatg
      481 aactggtcga acagctgctc gagtacaaga aaatgcagca ggcggcagaa gagatgcgcc
      541 ggctcgaaga acgctcccag ttacggctga cccgcgaacc acagtggagc gcctttgaga
      601 gcgacagcga ttacctcgag gtcgacctgc tctctttcct gcggacattc cgggattttc
      661 tcgagcgcga agaaaaatcg cgcccgatgc agatacagga cgaaacgatc agcgctgaag
      721 aaatgatgga gtacatgatg tcgcgcctga tgggcgacac ggaagcttct ttctttcgca
      781 gcgtgacggg gttcagcgtt gcccgcatca ttgcctgttt tctcgcgctg ctcgagctgg
      841 ccaaacagaa gatggtagtc cttcgccagg acgagtggct gggcgacatc cattttgcac
      901 cggcgccctc ttacgacggc cagactgaac tgccattcgg agcaacatga gcgatacagc
      961 ctacctgaaa gccgcaattg aaggtctgct gtttatttcc ggcaacgggc tcactgccgc
     1021 cgaaatcgca aacggcctga acgaaaatgc ggagcaggta aaaatcgcgc tcgattcgct
     1081 gctcgacgat tatgccgagc gtgaatcagg tattgcgatc caggagattt cagggcggta
     1141 ccagttcacc acccaaccga atatttaccc ggcaatacag aatttcctgc ggcagaagaa
     1201 gaaagagaca ctgagcaagt cgacgctaga aaccctcgcg atcatcacct acaaacaacc
     1261 gatcacactg cccgaaattg aagagattcg cagcgtcagc tcgagagcac agctcatcgc
     1321 gctgcagcaa aaaaaactcg tcaaggccat gggccacaaa gagaccgtcg gcaagccgac
     1381 gctttacggc acaacgaagg actttctgcg gtacttcggc ctcaacagcc tggacgaact
     1441 gccaccgcca caggatgtga aggagctgaa ttttgacgaa ctctaaccgg ccgtatcaaa
     1501 tcctgccccg ctccggagcc ccgacatgaa gcagtaccgc gaccagatag accgcatcga
     1561 cgctgatatc gtacgcctga ttggccagcg cgcagaaatc gcccgcgaaa ttgcagcagc
     1621 aaaggccaag gataacctgc cgctctatca acccgaccgt gaacaacagg tctacgacaa
     1681 gatagcccgt ttgaatccgg gtatactcga cgatgaatcg ctgcgcaata tttaccgtga
     1741 aatcatgagt gcaaccctca agcttgaagg ctctatccag gtgggttact tcggcgccga
     1801 aggcagcttt acccaccagg cggctttgcg caagttcggc cgcagcctca gcctcgcgcc
     1861 gttcagaacg atcgatgacg ttttccaggc ggtgcagcgg cgcgagatca agtatggcgt
     1921 cgtcccgatt gaaaactcca ccgagggcat ggtcaaggca accctcgatg cgctggtgaa
     1981 gttcgacctg aatatctact cagatattgt tttgcagatc aggcaaagcc tgctcacgcg
     2041 cgcaaccgaa ctttcgcaga tcgaaaagat ctacacgcac ccccaggcgt atgcacaggc
     2101 gcgcaacttc atcatgaaga acctcgcaca ggccgagtgg attgaaaccg cttcgacatc
     2161 agaagccgtg cgcctagtcg cacaggagaa cagcgagcgt ctcgccgcga tcgcctcgga
     2221 cgtggccggt gaaatttatg gcctgcgtcc tctcgtcgaa gatatcaccg attataaacg
     2281 caatttcacc cgttttatcg tgatcggcca cgacgtggcc aaaagggcag aacgcaaccg
     2341 cacgatgatt tcatttaacc tgccggacac caccggttcg ctctaccgcg cactcgcacc
     2401 gctctacgaa cagaaagtga acatgcgcgc cattgaatcg cggcctgacc gcaaccaggt
     2461 ctggagctat attttcttcg ttgatctcga aggccaccag gaagacgaag cgatgaagcg
     2521 cgcatttgaa ctcatgcgcc cattgacttc aggcttccgc gtactcggtt cttaccccgt
     2581 ggaaaaaggc ccggaagact agctagtaaa tagcccttag ccgctgcggg tgtatgccaa
     2641 tcgtgatatg ttcgcccaac gccacgggca tccggtggtg tgaaccatca agccagagcg
     2701 cgccgtgcat caggctgctg acaaccttga gcgtttcgcc tgcccttaca gtgccatgcg
     2761 tcaggcgccg cggtgcggca tcgctgtaaa gttcgcgcac aataaagcgc agctcgcgcc
     2821 gcgtgaatgg tttgcccccg gctgacgcat acgcggccgt cgacccggta ggtgtcgtca
     2881 cccaaatacc ggagcttttc tggtggtgaa cgcggttgcg ccagcgcagg gtgtagcgcg
     2941 aggttgccgc agggttctgc tcggaaaaca ggacgtcgtt caggacagga aacggcgccg
     3001 gccggtcctg cagggtcacc ttgagccgca tcagctggct ttcggccggg cgttttgccg
     3061 atgactcgag caacgccagc aagtcgcgcc gcagcctggt ctcaccctgc ttgctgcgca
     3121 gctcgtagcg gcagtaatgc ccgacgctgt tgtcaggcga cgaattgatg ccaagcaaga
     3181 gcgtcttttc gatgaagtgg gacgaattga tgaaagtacc gtcgccgccg aaagaaatga
     3241 ccagccggaa gtcgtcgagg ctgcggttca gcgtatcgcg ctcgaaccag ctggcaccgg
     3301 cgcccagctg cgcaatcagg cggcgcagcg actcgagcgc gccggcatgc tcttcatgcg
     3361 ccgactgcat gcgctgcgtg agttcaggat tcagctcgcg gtggcgacga atctgccgag
     3421 ggctgtagct ctcgagcgcc gagtatttat aaacgactgc aatgtctttc atggcggacg
     3481 cgttagtgca cgcaactctg cgcaggcgtc catcacttgc gtaaaatcac gttgagcata
     3541 cgctccgtcg cgcgaatcgc cgcaatgacc tggtcggggt caaccccgcg tgttgtgagg
     3601 atattgctgt cacggccgac cgtgagctca ccgccgaaca tcgccttgtc gctgtccgtc
     3661 ttttctttgg cggctgcgag gcctgcaatg ttccaggtga tcttgcattc gacgagcgcc
     3721 gaggtctggc caccgggcgg aatacgcact tcgtaatcgg tgaggcgtgg cagattaatc
     3781 ttatgttctt ttgcccagct gcgcagcgct gacatgaagg cgtcatagcc accctcgccc
     3841 tgccctgaaa cctgtaccgc cttgccgcgg tactcgaccg tgagtgagca catcggcttg
     3901 atatgcatgc ccgaggtgac tgtcacctgc gtgatttcca gaatcttctc ttcgggcttt
     3961 ttcagcacct cgccaatgat aaaaggcagg tcgtcgatag tcacctgctt cttctggtcg
     4021 cccagctcga taatacggct caagacctgg gcgcgttctt catcggccag cgagataccc
     4081 agcgtcttaa ggttctggtc gagcgatgct ttgcccgcga gtttacccaa agcgtaacgg
     4141 cggtcgcggc caaaacgcgc tggcaagagg cggctttcat acaggccgcc tttcttgtcg
     4201 ccgtcggcat gtataccggc cgtttgcgta aaaacatctt cgccgacaat gggcgcattg
     4261 gccgcgaggc gcttacccga aaaagtctct accgtgcgcg acagttggta taggctcttc
     4321 tc
//
LOCUS       DAOQJF010000283         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12322_length_4310_cov_4.248179, whole genome shotgun sequence.
ACCESSION   DAOQJF010000283 DAOQJF010000000
VERSION     DAOQJF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12322_length_4310_cov_4.248179"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2469)
                     /locus_tag="PLF85_13295"
     CDS             complement(<1..2469)
                     /locus_tag="PLF85_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20693.1"
                     /translation="MLAKRPPYFSLFSVTPALLGLVLLPACSGLKLDKESNCLNDKTA
                     CYKEDKTRPRVQTFTTQPPLDGANSLTELQHIDVTFSEEIKDGELAQTYKFKPSAGHS
                     FNVTSVQRLSTYTYRLIVGGGLQNTGLIEVDIAALKDYNENPMDTIVPVEFTGNVNIP
                     IVSSVNHNGVSTTGGYASVNLKFYHNYTADVTNANTYQVRLTSGAVDCSGGTLLTSGS
                     NFAPGSANEITWNVPAASFASGMNRLVVCISNANNPNAANSASWPIIRDDVAPALTYT
                     PAGGSYKDPQAITLSCGNNADKIAYTSVSQQGSVPTDPAAPTFDPATGNIVTGNTYGT
                     GLTAENPANPTYTKFIWRCIDIAGNLSTALATPVQFYIDNTIPGVTVNLASGYRQFVS
                     TVNPTSALVFTTDQVSKAYNIRRNGTNCDGGGDGTLLATGTTPGTAGQTISEPLDITT
                     HFTATNTTYPVRICVAGTGIQWGTAYLEITRDDTVPTITPSVVTGDYGALQSVQFTCD
                     DAFGSVHRVAYKMTEADGLTLPALPTVPTYNTATGAITDGTEQITALSPLDQKTTRYT
                     FNCIDRAGNVATAGSTRYTIDATLPVVNFVSLSRTAVSGNAGAFADTTLTFYSNRAGL
                     TYRIRRVANCDHTGTPAETIDSGTIGAVNANQSALLPAGAFPNNGQTYPLRICVYNLL
                     GASSHQTASVNVTRDTTAPLTFAGLTSLTSGGAGQFTLNWSAADDGAGSGIAAYNIYR
                     ADSASGPWTTIHHVAAHPAGSINVTVPDPLLPYYYVAGAVDNAGNETKVTASPLATKP
                     EVRLIVSGLGAGKSFTFTDGTGATA"
     gene            complement(2564..3598)
                     /locus_tag="PLF85_13300"
     CDS             complement(2564..3598)
                     /locus_tag="PLF85_13300"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNE20694.1"
                     /translation="MQPIIKSVLLLLLSGSALLAQSGTTFKADKPVVIQADNDNPAAP
                     AIKKLIFLDFYNESNDPNVKWLTDSIGESIFELTKNKYKYVRIESKVWREYAKSQSFK
                     PEDFYDIDKLQAMGFALKADGIIFGKFRSSPENIEISGKILSVVDKEIVAEKNITVPF
                     SSQMFEDVQDVSETLGSRIKDLFYPSDRGALWRSALLPGWGQFYKQRKTAGYIYSSVI
                     GTGAAFSLFSFIMLQSTKQQYASYNPDHVVTPQGEFGIKDPAATQAEFARLEAKADQW
                     YQITMISAGVTLGIYFWHLFDAWFFEGNYVELGKSKAMDFHSGESLLGSKVRISGDRA
                     PVSVRFDYAF"
     gene            3780..>4310
                     /locus_tag="PLF85_13305"
     CDS             3780..>4310
                     /locus_tag="PLF85_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNE20695.1"
                     /translation="MKKFNLSLLFVAVAALNGSAFAAVPKTTYPVVFAHGMAGFDDLL
                     GYSYWGDDYGVFVGDPCDEFLEVTCNGNINSGQRAFQTQTFPFQSSEVRGLDIANQIE
                     SYMTSVGASYINLVGHSQGGMDIRKAAKVLYQRKGRQVVRVLISISSPHRGSPVAKYI
                     LDLGPGVSGVVSALATI"
BASE COUNT          963 a         1114 c         1188 g         1045 t
ORIGIN      
        1 ggcagtcgcc cctgtaccat ctgtgaaagt gaaacttttg cccgcaccca ggccgctgac
       61 gatgaggcgc acctcgggct tcgttgctaa tggcgaggct gtcactttcg tctcattgcc
      121 cgcattgtcc actgcacccg cgacatagta gtatggcaac agcgggtcgg gtacagtcac
      181 gttaatcgaa ccggcagggt gcgccgcgac atggtgtatc gtggtccacg gccccgatgc
      241 gctgtctgcg cgataaatat tgtaagcggc tatgcccgag cctgcgccat cgtctgcagc
      301 gctccagttc agtgtgaact ggcctgcccc gccgctcgtg agggaagtga ggcccgcgaa
      361 ggtcagcggc gcggtagtgt cccgggtgac attgacactc gccgtctggt gactgcttgc
      421 acccagaaga ttatacacac agatgcgcag cggatatgtc tggccgttat tggggaaggc
      481 gccagctggc agtaaagcgg attgattcgc attgactgca ccaatggttc cagagtcaat
      541 cgtctctgca ggcgtacctg tatggtcgca attcgccaca cggcgaatac ggtacgtcaa
      601 acctgcacga tttgaataga aggtcagtgt cgtatcagca aatgctccgg catttccact
      661 gacagctgtg cggctgagac tcacaaagtt cacgactggc aaagttgcat cgatggtgta
      721 gcgcgtgcta cctgccgtcg ccacgtttcc ggcgcgatca atgcagttga atgtatagcg
      781 tgtggttttc tggtctaaag gtgaaagcgc ggttatctgt tcagttccat cggtaatcgc
      841 cccggttgca gtgttataag taggcaccgt tggcaacgcc ggcagcgtca ggccatcggc
      901 ttctgtcatc ttgtacgcga cacgatgtac gctgccgaat gcatcgtcgc aggtgaactg
      961 cacgctctgc agtgcgccgt aatctcccgt aactacagag ggagtaatcg tcggtacggt
     1021 atcatcgcgc gtgatttcga gatacgccgt cccccactgg atgcctgtac ccgctacgca
     1081 gatgcgcacc gggtaagtcg tgttagtagc cgtgaagtgg gtggtgatgt cgagcggctc
     1141 agatatcgtc tgaccggcgg ttccgggcgt ggtaccggtg gcaagcagcg ttccgtcgcc
     1201 accgccgtcg caatttgtgc cattgcggcg gatgttataa gccttggaga cctggtcggt
     1261 ggtgaacacg agcgcgctag ttgggttgac tgtcgaaaca aactggcgat aacccgaggc
     1321 cagatttact gttacgcctg gaatcgtgtt atctatatag aactgcacag gtgtcgccag
     1381 agcggtgctg agattgcccg cgatgtctat gcagcgccag atgaacttcg tgtaagttgg
     1441 gttggccgga ttttcagcag taaggcctgt gccataagtg ttaccggtga caatgttacc
     1501 agttgccggg tcaaaggtcg gtgccgcggg gtctgtaggc acactaccct gctgcgacac
     1561 actcgtgtaa gcaatcttgt ccgcgttgtt gccgcagctc agcgtgatgg cctgagggtc
     1621 tttatagctc ccacctgctg gggtataggt caatgccggc gcaacgtcgt cacgtatgat
     1681 cggccatgat gcgctgttcg cggcgttggg attattcgca ttgctgatac agaccacgag
     1741 ccggttcatg cccgacgcaa acgaagctgc cggtacattc caggtaattt catttgccga
     1801 gccgggggcg aaatttgaac cgctggtgag caaagtacca ccgctgcaat caacagcgcc
     1861 gcttgtgagc ctcacctggt aggtgtttgc attggtaaca tcagcagtat agttgtggta
     1921 aaacttcaga ttgaccgatg cataaccacc ggtggtgctc acgccattat ggttaacgct
     1981 gctgacgatg ggaatattga cgttgccggt aaattctacc ggaacgatcg tatccatcgg
     2041 gttttcattg tagtctttca gcgcagcaat gtcaacttct atgagacccg tattctgcag
     2101 gccaccaccg acgataaggc gatacgtgta agtcgacaac cgctggactg aggtgacatt
     2161 gaaactatgc cctgcgctgg gtttaaattt gtaggtctgc gccagctccc cgtctttgat
     2221 ttcttcgctg aaggtgacgt cgatgtgctg cagctcggta aggctgtttg ccccgtcgag
     2281 tgggggctga gtcgtaaacg tctgcacccg cggccgagtc ttgtcttctt tgtaacacgc
     2341 cgtcttgtcg ttcaggcagt tcgactcttt gtcgagcttg agacctgaac aggccgggag
     2401 cagcaccaaa cccaacaagg ccggtgtaac agaaaaaagc gagaaatatg gcggtctttt
     2461 ggcgagcata gtttagcgtg ggtatctggc ggggccaaaa aagtgagcac ccggcactat
     2521 aacttgcggg taccgggcag aatgtccggt atttttttct cctttaaaaa gcataatcga
     2581 agcgcaccga tacaggagcc cgatcaccgg aaattcgcac cttgctaccg agcagacttt
     2641 ccccgctatg gaaatccata gccttggact tgcccagttc aacataattg ccctcaaaga
     2701 accaggcgtc aaacaggtgc cagaagtaga tgccgagggt aaccccggcg ctgatcatcg
     2761 taatctggta ccactggtcg gccttggcct cgaggcgggc aaattctgcc tgcgtggccg
     2821 ccgggtcctt tattccgaac tccccctgcg gagtcacgac gtgatcaggg ttgtagctcg
     2881 catattgctg cttggtcgat tgtagcataa taaaggaaaa aagcgaaaag gctgcgccgg
     2941 tgccgattac actgctgtaa atataaccag cagtcttgcg ctgcttgtag aactgtcccc
     3001 aacccggcaa aagcgctgaa cgccaaagag ctccccggtc tgatgggtaa aagaggtctt
     3061 taatacggct gccgagtgtt tcagagacat cctgcacgtc ttcgaacatc tggctcgaga
     3121 atggcacggt gatatttttt tcggcgacaa tttctttatc aacaaccgag agaattttcc
     3181 cactgatctc gatattttcg ggggaggatc gaaacttgcc aaagatgata ccgtcagctt
     3241 ttagggcaaa tcccatggcc tgcagcttat cgatgtcgta gaaatcttca ggtttgaaag
     3301 actgggattt tgcatactcg cgccagacct tactttcaat gcgcacgtac ttgtacttgt
     3361 tctttgtgag ttcaaaaatg gattcgccga tcgagtcggt cagccactta acattcgggt
     3421 cattcgactc gttgtagaaa tcgagaaaga tcagcttctt gatcgcaggc gctgcagggt
     3481 tatcgttatc agcctggatg acaacgggct tgtcggcttt gaaggttgta cccgactgtg
     3541 ccagcagcgc agagcctgag agcaaaagta acaggacgct ttttattata ggctgcataa
     3601 cgaaactgtg tgcgtcgcat aacgccaaaa caacgaagcg taaagcagaa tgacaggtcg
     3661 accattgcgc aacgcacaat tgatattgaa caactgttca tacttttttt ggcccattac
     3721 ccaccacagc atccatatag acatggcttt tgaaaaaagt gtgaaggaga aaaaatgtaa
     3781 tgaaaaaatt caatctgtca cttctctttg tggcggttgc agcgcttaac ggctcggcat
     3841 tcgcagcggt tcctaaaacc acatacccgg tggtgtttgc tcatggtatg gctgggtttg
     3901 atgacctgct gggctattcg tactggggcg acgactacgg tgtattcgtt ggcgatccat
     3961 gcgatgaatt tcttgaagtt acctgcaacg gcaacatcaa cagcggccag cgcgcattcc
     4021 agacgcagac ttttcccttc cagtcatcag aagtgcgcgg tctcgacatc gcaaaccaga
     4081 ttgaatctta catgacatct gtcggcgcaa gctacatcaa ccttgtgggc cattcgcagg
     4141 gcggtatgga tatccgcaag gcagccaaag tgctctacca gcgcaaaggt cgccaggtgg
     4201 tacgcgtact gatttccatt tcttctcctc accgcggatc gccggttgcc aagtacattc
     4261 tcgacctcgg tccgggtgta tcgggtgtcg tttctgctct cgcgaccatt
//
LOCUS       DAOQJF010000284         4303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12348_length_4303_cov_4.334040, whole genome shotgun sequence.
ACCESSION   DAOQJF010000284 DAOQJF010000000
VERSION     DAOQJF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12348_length_4303_cov_4.334040"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLF85_13310"
     CDS             <1..265
                     /locus_tag="PLF85_13310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20696.1"
                     /translation="AHLAIKSDRLAEAAQYLERYSDACPGDTQAIETLGTILTRLGEY
                     DKALDVLNRLRIREPQDERMRQVVARVLKLQGKTHAAEELLAG"
     gene            complement(285..1235)
                     /locus_tag="PLF85_13315"
     CDS             complement(285..1235)
                     /locus_tag="PLF85_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20697.1"
                     /translation="MRAFYLRGLPAFLAFVISLASCQSTSTSNYPDKINQRNFTIDAS
                     GGSYGVTLFPRTYSALAGSSFGLVIVSPVLLNDEILYNSGSSSIIPWFNARGISVWLV
                     RIPPQTNLEQFGRETLPVVTAAIRKNSNDDAWVMGGVSLGGQAVAHYLAEAPRNATVS
                     GMLVKAAFFLGTGFDYNYPDSFTRRLVAAPANLCSASFCGNYLPGMPAGLVQAKSALF
                     DSKGKPVWRDAINGTALRDKGVRLFIASGKIDNVAPSEAVYRFYTQALGDERKVSPDV
                     RFLQPGRMNRHAADFNHSMMVASPELAADVLPEVLRWLDL"
     gene            1382..2605
                     /locus_tag="PLF85_13320"
     CDS             1382..2605
                     /locus_tag="PLF85_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20698.1"
                     /translation="MATLADHNKNIHQVADMEQKAIPKGFIDLPANIILSADGFQVLS
                     ASKTPIRRLTARGGQARDGLFSQAFNAATIQKMIMNSYIDEIYYASGEFLTKRPQIIS
                     TTNLVIYAMLYKMLSPTLARRVIESPVLREYNRRNPKNAIVDFKSIPQKVMDDFLTKN
                     KAILARIEHEMIDAVLTRITSDKNLPEEDKIVRMRSLEKFVRWVDKRIWYLYLIIYQT
                     PLKKEIETAYVELFFQYLDNTQVAVHTGNLVMEFIQNAEKAHLERVIVRNQLASRDQV
                     DAYLRNKLHREQVVAMAERSKQQLEIAWHFQTDRVNAGKIYNVKIIISNFGLINESTR
                     DSLAKKLKTDTSGISLSDFYEDSGETGKLGAGLGLLYNSYLEDVCREKGIRYFCTIFP
                     EPRLEKTTVTLDLTF"
     gene            complement(2583..3101)
                     /gene="nusB"
                     /locus_tag="PLF85_13325"
     CDS             complement(2583..3101)
                     /gene="nusB"
                     /locus_tag="PLF85_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNE20699.1"
                     /translation="MAKSIRQHRHKARILAMQALFQYDTRGTERPTLEQLLTFNWMDY
                     AVPEEEQEFARAIIRQTVEHIDEIDTQITERLVGWEFSRLSPVNRAILRTGVAQLKYM
                     AQDADAPVVIDECIQLSRKYDDVQSAAFVNGILDSVQRGAAPGTGTPAKIPVLKEKIR
                     LKKPVAKKSNPK"
     gene            complement(3103..3579)
                     /gene="ribH"
                     /locus_tag="PLF85_13330"
     CDS             complement(3103..3579)
                     /gene="ribH"
                     /locus_tag="PLF85_13330"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR00114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNE20700.1"
                     /translation="MQISDRHYKEPIQDHTLRVAVITALFNEEITTALEKGAVDELVS
                     LGVDAARIQVVRVPGAWELPYAAGRLLRENKADAVIACGCVIRGETSHYDIVAGESAS
                     GLMRVSLDFSRPVMNAVLTVENTAQALARCAAGPTNKGAEAARSLVELANAFAGAR"
     gene            complement(3589..3661)
                     /locus_tag="PLF85_13335"
     tRNA            complement(3589..3661)
                     /locus_tag="PLF85_13335"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3626..3628),aa:Ala,seq:ggc)
     gene            3779..4291
                     /locus_tag="PLF85_13340"
     CDS             3779..4291
                     /locus_tag="PLF85_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20701.1"
                     /translation="MSTRRVIFSLFLLSATAMLSANEKTGQANISDEMEAYRAIQNRP
                     VTTVQDLADLLLMYRGEYAKFTSQAKRLERVRELGLLQKEHEGNEELDRGTLAFALMQ
                     VYQPEHGWLYWLTGWERYALRDVQEAGIIPSKSTPTQKMSGDQLLAIMTDAEEFVTKR
                     SEWQKEEKEQ"
BASE COUNT         1064 a         1205 c         1165 g          869 t
ORIGIN      
        1 cgcgcacctg gcgataaaat cagaccggct cgccgaggct gcacagtacc tcgaacgcta
       61 tagcgacgcc tgccccggcg atacgcaggc tatcgagact ctgggaacta ttttgacgcg
      121 actcggcgaa tatgataagg cgctcgacgt actcaaccgc ctgcgcatac gcgagccgca
      181 ggatgagaga atgcggcagg tggtcgcgcg cgttctgaag ctgcagggca agacccacgc
      241 tgcggaagaa ttgctcgcgg gttaacggat actgaacctc tgggttagag atcgagccag
      301 cggagaactt cgggcaacac gtcggctgcg agttcgggcg atgccaccat catgctgtgg
      361 ttaaaatccg ccgcatgccg gttcatgcgc cccggctgca gaaagcgcac gtcaggcgat
      421 accttgcgtt catcaccgag agcctgcgta tagaaccggt aaacggcctc tgacggggcg
      481 acattatcaa ttttgccaga ggcaatgaac aacctgacac ccttatcgcg cagcgctgtg
      541 ccgttaattg catctctcca gaccggctta cctttactgt caaaaagtgc cgactttgcc
      601 tgaacgaggc ccgcgggcat accgggcaaa tagttcccgc agaaactggc tgaacacagg
      661 ttagctggcg cagccacaag ccggcgcgtg aaggaatccg ggtaattgta atcgaagcca
      721 gtaccaagga aaaacgcagc tttcacgagc atgccagaga ccgttgcatt gcgcggcgcc
      781 tcagcaagat agtgcgccac ggcctggccc ccaagcgaga ctccccccat cacccaggca
      841 tcgtcgttgg agtttttccg aatggctgcg gtcacaaccg gcagagtttc gcggccaaat
      901 tgttcgaggt tcgtctgcgg gggaatacgc accagccaga ccgagatgcc gcgagcattg
      961 aaccacggta tgatcgagct tgaacccgaa ttgtagagta tctcgtcgtt caggagtacg
     1021 ggagatacta tgaccaagcc gaacgaagag ccggcaagtg cactataggt gcggggaaac
     1081 agcgtgactc catagctacc acccgatgca tcaatcgtaa aattgcgctg gttgatcttg
     1141 tcggggtaat tgctcgtact tgtggactgg caggaggcaa gagaaattac aaaggccagg
     1201 aaagctggca ggcctcgcag gtaaaaagca cgcataaggg cctgactgcc gctcgtctgc
     1261 ttgatgtaaa tagttattat gccgcgcgcc gcattcctgg gcaaatcttt ttgttttcgc
     1321 agaggctcgg acaccggaca ggtcactgga aagtcaaaca catgggttgt gacctcatgg
     1381 cgtggccact cttgccgatc ataataagaa tatccaccag gtagcagaca tggagcaaaa
     1441 agcaatcccc aaagggttca tcgacttacc agccaatatt attctgtctg ccgacgggtt
     1501 ccaggtgctt tcggcctcta aaacgcctat acgacggctg accgcgcgtg gcggacaagc
     1561 ccgtgacggc ctcttctcgc aggcattcaa tgccgcgacg atccagaaga tgatcatgaa
     1621 ctcgtatatc gacgagatct actatgcttc aggcgagttt ctgaccaagc ggccgcagat
     1681 tatcagcacg acgaacctcg tcatctacgc gatgctgtac aaaatgctct cccctaccct
     1741 ggcgcgcaga gtcattgaat cgccggtctt acgcgaatac aaccggcgca acccgaaaaa
     1801 cgcgatcgtt gactttaaat cgattcctca gaaggtgatg gacgattttc tgacgaagaa
     1861 caaggccata ctcgcgcgca tcgagcacga gatgatcgat gcggtactga cgcgcatcac
     1921 ctcggacaag aacctgcccg aagaggacaa gatcgtccgg atgcgcagcc tagagaagtt
     1981 cgtccgctgg gtggataagc gcatctggta cctgtacctg attatctacc agacacccct
     2041 gaagaaagag atcgagactg cttatgtcga actctttttc cagtacctcg acaacacgca
     2101 ggttgccgtg cacaccggca acctggtgat ggagtttata cagaacgcgg aaaaagcgca
     2161 cctcgaacgc gtgatcgtcc gtaaccagct ggccagccgc gaccaggtcg atgcgtacct
     2221 caggaacaaa ctccaccgtg aacaggtggt ggcaatggcc gagcgcagca aacagcaact
     2281 cgaaatcgcc tggcacttcc agaccgaccg cgtgaatgcg ggtaagatct ataacgtgaa
     2341 gatcatcatc tcgaactttg gcctgatcaa tgaaagcacg cgcgactcgc tggcgaaaaa
     2401 actaaagacc gacacctcgg gaatttctct gtctgatttt tatgaagaca gtggcgaaac
     2461 cggtaaactc ggtgcgggtc tggggctgct gtacaactcg tacctcgaag atgtctgccg
     2521 cgaaaagggc attcgctact tctgtacgat ttttcccgag ccgcgcctgg agaagacgac
     2581 agttactttg gatttgactt tttagcgacc ggcttcttga ggcgtatctt ttctttcagc
     2641 acgggaattt tcgccggggt accggttccc ggtgcagcac cgcgctgcac gctgtctagt
     2701 ataccattga cgaacgcggc agattgcacg tcatcgtact tgcgagaaag ttgtatgcac
     2761 tcgtcgatca caaccggagc gtcggcatcc tgcgccatgt acttcagttg tgcaacgccg
     2821 gtgcgcagaa ttgcacggtt aaccggtgac aggcgcgaaa actcccaacc aacaagtcgt
     2881 tcggtgatct gcgtatcaat ttcatcgata tgctcaacgg tctgcctaat gatggcgcgg
     2941 gcgaactcct gctcttcttc agggactgcg taatccatcc agttaaaagt gagaagctgt
     3001 tcgagagtgg ggcgctctgt cccgcgtgtg tcatactgga acagcgcctg catcgcgaga
     3061 atgcgcgctt tgtggcggtg ctggcgaatg gacttggcca ttttagcgtg ccccggcaaa
     3121 cgcgttggcc agttcaacga gcgagcgggc ggcttcggcg cccttgttgg ttggccccgc
     3181 agcgcagcgc gcgagagcct gcgcggtatt ttcaaccgtc agcacggcat tcatgacggg
     3241 acgtgaaaaa tcgagagaaa cgcgcatgag gcccgaggca ctctcgcccg ccacaatatc
     3301 gtaatggctg gtttcgcccc gaatcacgca accgcacgcg ataacagcgt cggccttgtt
     3361 ttccctcaga agacggcctg cagcataggg gagctcccac gcaccgggaa cacgcaccac
     3421 ctggatgcgg gcggcgtcta cacctaacga taccagctcg tcaactgcgc ccttctccag
     3481 ggcagtagta atttcttcat taaaaagcgc ggtaattacc gcgacacgca gggtgtgatc
     3541 ctgaatcggt tctttgtagt gccggtcgga tatctgcata atttcctttg gaggttaggg
     3601 gagttgaacc cctgaccttc acaatgccat tgtgacgctc tcccaactga gctaaacccc
     3661 cgaaaactct atgaactgtg gtggggccaa gccccgcaac atttcagaga aattcgacac
     3721 gccgtcaatg cgttatgaaa ttccttgaca ggcagaaaat cagagcatcc actgctctat
     3781 gtccaccaga cgcgtcatat tctctctttt cctgctctcc gcgacagcaa tgctttcggc
     3841 aaacgaaaag actggccagg caaacattag cgatgaaatg gaggcctacc gcgcaatcca
     3901 aaaccgcccg gtaaccacag ttcaggatct cgcagacctg ctactgatgt acaggggcga
     3961 atacgcaaaa tttacctcgc aggcaaaacg cctcgaacgc gtgcgcgagc tgggcctgct
     4021 acaaaaggaa cacgaaggca atgaggagct tgatcggggc acgctggcct tcgccttgat
     4081 gcaagtctac cagcccgagc atggctggct ctactggctc accggctggg aacgttacgc
     4141 cctgcgcgat gtgcaggaag caggtattat acccagtaaa tcgactccga cacaaaaaat
     4201 gtcgggcgat caactacttg caatcatgac cgacgctgaa gaattcgtca ccaaacgcag
     4261 cgaatggcaa aaagaggaga aagaacaatg accgcaaact caa
//
LOCUS       DAOQJF010000285         4286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12428_length_4286_cov_3.659655, whole genome shotgun sequence.
ACCESSION   DAOQJF010000285 DAOQJF010000000
VERSION     DAOQJF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12428_length_4286_cov_3.659655"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1034)
                     /locus_tag="PLF85_13345"
     CDS             complement(261..1034)
                     /locus_tag="PLF85_13345"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE20702.1"
                     /translation="MKRLLLLIIYSTGLAHALERISDKTGYGFEILHKEDRIFYVWYR
                     KNANIRHLSIDRLQSESYSLDVTFEEENGNLSNITMRKANKSKKQSAPVGRHIYFGDQ
                     GEIGRYFYASGTDGGYGIAEPCDVEYWYDDAGQVHKEKKTIHKRPCKYGKGNVAFFTP
                     PGEYYVTEGPLRVRKEPNLKSPIVVTLANDEKLTVTDFTTERLEINGSYAPWSKVKTA
                     KGEGWVYGAYIDPVDWNKTYTSYFKNIDWQAFVKKLGLE"
     gene            complement(1046..3421)
                     /locus_tag="PLF85_13350"
     CDS             complement(1046..3421)
                     /locus_tag="PLF85_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20703.1"
                     /translation="MVQTMALEQAFKLAGGQRGKKGYYQEYSGERALWKGYGGDLISE
                     GSGEQGGWVNGQKWNKLAWRGLQEEESKLAKARADWQTAAAVTNGAIVAATGVIASIM
                     TGGAAAPAVIGLLAAAQTTTIQVGSSFSSNRADQAWGQLQMGILQMGISGLGAFLGRI
                     DYLADAGKLTKVANAMTKVMAQNAVSYAGSGFQMSEDGSIAYKAPSKGALYSTLVSTG
                     FGAAGAGINAGVGWGSLTSGVVSTTANAIGAGFQFNDDGSSKAYDWKKTGTSFASNLA
                     SVTASYYATGALNRAGINDEVNEVLSNQIGRAANVVTGSLLGDENAVNAYRVQGLFNT
                     IGTNIGDILGHRANGGKGTGEPLRELLAGSAILTARREQELADQSTDPKSGAKKISDP
                     MADLFGAIGSGIASGASSVWSGIKSGASAVASFFSNAASSVASFATNTFERFGNLVSG
                     AGFNTNSQVEAIRNETNSNVADTMQLTPGYAAVANRLGGKLTNVDGNPAVIKPDGTFE
                     FINDKDAIAAEADMLGMSAAQTRGGRVNLLGYDGFDLSEEDAFAMESALARDALASTN
                     RQKKAGINNFVKKSQSTKIDGITIKFKGELDGSTKLKAGQAALWKNLVEAAKAAGVTE
                     ITVGSVERSAGTHVDGYSADVLNVTFAGESKPTAYNRETQGAQRAKLEVFEQAFIKLA
                     DSEAAWTPNQMLSNSTGKAASKQGYDADFYKGSNGQMGSNFVDEYPSIAARAQQIKNT
                     NASDAYKILAQVKKEFGKTIKDESVLKAITGSIQHVDHGHFMVRRPIYRPI"
     gene            3516..4244
                     /locus_tag="PLF85_13355"
     CDS             3516..4244
                     /locus_tag="PLF85_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNE20704.1"
                     /translation="MSSIKNTSFLEAVAAGIQHEKDFFDFCMKTHDELDGGPVKNFFF
                     DLAEDSVEHLKMIEGIYQKYSGNQALPNLKHVGQVHKFHATAIQKLIKKLDRNLSKSA
                     KGNELEALRLALQEAEDAADAFAKLADKFSDAGIKMLFQKISQFNKERAILLEGTLVY
                     QYPMADERAVQEYHLEVVSVQIEPQSVRAEKIINARAIPVKKNKAKKPAKKTVPKKKP
                     VPKKAAKKATKKAKPAKASGKKKK"
BASE COUNT         1022 a         1247 c          995 g         1022 t
ORIGIN      
        1 tgcgcttcgc ttcggcccac gcgcgcgtgg gctaaaccct tcgagcccct aacgctttgt
       61 gctgcggttt gggccagtgg tgcataagtt gtcatggttg tatgtatttt gccggcgcca
      121 aactcaaacc ccgccccttc tcttgaaaag agaaggggcg tccgaaactc ccctctcccc
      181 gtgggcgacc gcaaggtctc cgccttcgga gaggggtcgg gagtgagggt tggcgccggg
      241 ctgacgcgcg ggctggcgaa tcactccaga cccaatttct tgacaaacgc ctgccagtct
      301 atattcttga agtaactagt ataggtcttg ttccaatcaa ccgggtcaat gtaagccccg
      361 tagacccagc cttcgccttt ggctgttttc acctttgacc acggtgcata tgaaccattt
      421 atctccaaac gctccgtggt gaaatcggtc actgtcagct tttcgtcatt tgcaagcgtc
      481 acgactatgg gcgactttag atttggctct ttgcgcacac gcaacgggcc ttcagttacg
      541 taatattcac cggggggcgt gaaaaacgca acattacctt tgccgtattt gcaagggcgt
      601 ttatgtattg tcttcttctc tttatggacc tgacctgcgt cgtcatacca gtactcaaca
      661 tcgcaaggct ctgcgatacc gtagccacca tcggtgccgc tggcatagaa gtatctgcct
      721 atttcgccct gatcgccaaa atatatatgc cgaccaactg gcgcactctg ctttttcgat
      781 ttgtttgcct tccgcatcgt aatattcgag agattgccat tttcctcttc aaaggtaaca
      841 tctaatgaat agctttcgct ctgaagccgg tctatagaca agtgcctgat atttgcattc
      901 ttgcgatacc aaacgtaaaa gattcggtct tctttatgca agatttcaaa gccataccct
      961 gtcttatcac tgattctctc taatgcatga gccagccccg tgctgtatat gatgagtaat
     1021 aagagtcttt tcattttacc atcctctaaa taggccggta tatcggccgc cgcaccatga
     1081 aatgcccatg atcaacatgt tggatactgc cagtaattgc cttaagaaca gactcgtctt
     1141 tgatagtctt cccaaattct ttcttcactt gtgccaagat cttgtatgca tccgaagcat
     1201 ttgtattctt aatttgttgg gctcgcgccg cgatacttgg atactcatcc acgaaattag
     1261 agcccatctg tccgtttgag cctttataaa aatcggcgtc ataaccttgc tttgatgctg
     1321 ctttcccagt cgaattcgat agcatctgat tcggtgtcca agcggcttcg ctatccgcaa
     1381 gttttatgaa cgcctgttca aaaacttcta acttcgctct ttgtgcgcct tgggtttctc
     1441 tattgtacgc cgttggctta ctttcgccgg cgaaagttac attcaggaca tctgccgaat
     1501 aaccgtctac gtgtgtacca gcggaacgct caacgctgcc cacagtaatc tccgtaacgc
     1561 ccgccgcttt tgctgcctca acaagatttt tccaaagagc ggcttgacca gcttttaatt
     1621 ttgtgctgcc atctaactcg cccttgaatt tgattgtgat accatctatc ttcgttgatt
     1681 ggcttttttt gacaaaattg tttatgccag ctttcttctg gcgattcgtt gatgccagcg
     1741 cgtctcgtgc cagtgcgctt tccatagcga atgcatcttc ttcactcaaa tcaaatccgt
     1801 cataacccaa taaattgact ctaccgcctc gcgtctgagc agccgacatc cccaacatat
     1861 ctgcttccgc agcgatcgca tctttatcat tgatgaattc aaatgtgcca tcgggtttaa
     1921 tcacagccgg gttaccatcg acattagtca gcttcccgcc caagcggttt gcgacagctg
     1981 cgtagccggg ggtgagttgc attgtgtcag caacgttact gtttgtttcg ttgcgaattg
     2041 cctcaacctg actattcgtg ttaaaccctg caccagacac aagattgccg aagcgctcga
     2101 aggtgttcgt tgcgaagctg gccacagagc ttgcggcgtt agagaagaaa ctcgccaccg
     2161 cgctcgcgcc actcttgata cccgaccaaa cgctggacgc gccgctcgcg atgccgctac
     2221 caattgcacc gaacaagtcg gccatcgggt cgctgatctt ctttgcacct gatttggggt
     2281 ctgttgattg atcggccagc tcttgttcac gccgtgcggt caagattgca gagccggcca
     2341 agagttcacg taggggttcg ccggtgcctt tgccgccgtt cgcgcggtgg cccagaatat
     2401 cgccgatatt cgtgccgatg gtgttgaaga gaccctgaac gcggtacgcg ttgacggcgt
     2461 tctcgtcgcc caagagtgaa ccggtaacga cattcgccgc acgaccgatc tggttagaga
     2521 gaacctcatt aacttcgtcg ttgatgccag cacgattgag cgcacctgta gcataataac
     2581 tcgctgttac tgatgcaaga ttcgaggcaa aagacgtacc ggtttttttc cagtcatacg
     2641 ccttcgacga accatcgtcg ttgaactgaa acccagcacc gatggcattg gctgtggttg
     2701 aaacaacacc cgaagtcaga gagccccagc caacacctgc attaataccg gcacctgccg
     2761 caccaaaacc agtcgaaacc agagtcgaat acaacgcacc cttactcggc gctttatacg
     2821 caatcgaacc atcttcactc atctgaaaac cagaccccgc ataagacact gcattctgtg
     2881 ccatgacctt ggtcatggca ttggcgactt tagttagctt acccgcgtcg gcaagataat
     2941 caatacgccc caagaacgcg cccaaacctg aaatccccat ttgcagaatg cccatctgca
     3001 actggcccca cgcctgatcg gcacggttcg aagaaaacga actgccgacc tgaatcgtcg
     3061 tcgtctgcgc ggccgccaaa aggccaatca ccgccggcgc cgcagcgccg cccgtcatga
     3121 tcgacgcaat cacacccgtc gccgccacaa ttgccccatt cgtcaccgcc gcggcagtct
     3181 gccagtcagc gcgcgccttt gcgagcttcg actcttcttc ttgcagcccg cgccacgcga
     3241 gcttgttcca tttctggcca ttgacccagc cgccctgttc gccagaacct tcagaaatta
     3301 aatcaccgcc ataacccttc cacagcgcgc gctcacccga atactcctga taataacctt
     3361 tcttgcccct ctgaccgccc gccagcttaa acgcctgctc cagcgccatc gtctgcacca
     3421 ttcgcggcaa aagccgccac gtccactcca aaaatgagct gacaatccgc ctcgcgctac
     3481 ccagtatggg taaccgatgg agtgaggagg gttacatgtc ttcaatcaaa aacaccagct
     3541 ttctcgaggc tgtggcggca ggcatacagc acgaaaaaga ctttttcgac ttctgcatga
     3601 aaactcacga cgagctcgac ggcggcccgg tgaagaactt cttcttcgac ctcgcagaag
     3661 acagcgttga gcatctcaaa atgatcgagg gtatctacca gaagtactca ggcaaccagg
     3721 cgcttcccaa cctgaaacac gtggggcagg tacacaagtt ccacgcgacg gccatacaga
     3781 agctgatcaa gaagctcgac cgcaacctga gcaagagcgc gaaaggaaac gaactcgagg
     3841 cgctgcgcct cgcgctgcag gaagccgaag atgccgcgga tgcatttgcg aagctcgctg
     3901 acaaattttc cgatgcgggc atcaagatgc ttttccagaa gatttcgcag ttcaacaaag
     3961 agcgcgctat cctgctcgaa ggcacgctgg tctaccagta ccccatggcc gacgaacgcg
     4021 ccgtgcagga gtaccacctc gaagtagtca gcgtgcagat cgaaccacaa tcggtgcgcg
     4081 cggaaaaaat catcaacgca cgcgccatac ccgtgaaaaa gaacaaagcg aagaaaccag
     4141 caaaaaaaac agtgccgaaa aagaaaccgg tacccaaaaa ggcggcgaaa aaggccacaa
     4201 agaaggcgaa acccgcgaag gcttcaggca aaaagaaaaa ataggagatt gggctgactt
     4261 aagccgagac gaggtcccgg ctttct
//
LOCUS       DAOQJF010000286         4277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12471_length_4277_cov_6.561345, whole genome shotgun sequence.
ACCESSION   DAOQJF010000286 DAOQJF010000000
VERSION     DAOQJF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12471_length_4277_cov_6.561345"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLF85_13360"
     CDS             <1..1011
                     /locus_tag="PLF85_13360"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNE20705.1"
                     /translation="TVSDSTRWQVIQSNCPALPAGLVLAAESCTISGTPTTAQAEVQY
                     TITGSNDFGSTSTQIGITVYIPPPSNLGYTGSPFTFSTYTSVGTITPTLNGTVTSCTA
                     SPALPAGLLLNGKTCTITGTATTYQNPVNHTITASNVLGSTTTTINIRVDECYARGCF
                     RDNSNGTVSFTGTGDGYAGVNLVFMKCSQGQVWNSLTNDCTGTGSAPNYGAATYASSG
                     GGCSSLSFAGRSAGSWRMPTGNELATLIHCTNRARVAYPGSCGAGNYTVPAISNLFPQ
                     TPSTGGTFPGRYWSGETFVPPGSPPTTFQYTSNFFDSTYSGARPNFGGFQMHVRCVAT
                     GP"
     gene            1098..1610
                     /locus_tag="PLF85_13365"
     CDS             1098..1610
                     /locus_tag="PLF85_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNE20706.1"
                     /translation="MKSTKPDYSKTLRVSNSPKHGRGVYAKQPFAKGAKVIEYVGKLI
                     PVKDSDAIAEKQMRRAGKSGTGHVYLFSLNARYDIDGNVPWNTARLINHSCDPNCEVY
                     IIKGHIWILAKRAIAAGEELTYDYGFNMANWREHICRCGTSKCLGYIVSASRRGALAR
                     VLKNIGKPQT"
     gene            1652..3373
                     /locus_tag="PLF85_13370"
     CDS             1652..3373
                     /locus_tag="PLF85_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNE20707.1"
                     /translation="MNTARAIYVLIVFSTAGLIFLSCSGYSTTQLLNAEFVTVNKDLS
                     PIDLLAPSDKSVSTKNPKLVWSRRAGAISYSVEMSAQSDFSSLLLKKSTTETSYSLNN
                     GDLISITTLDSFSYYWRVKAIYADQQVTSESFLFHVLDDTIVYVSSASTASDQVGNRS
                     APFKKIQSGIEAADTKRNGVATTPMFVYVAGGSYTEEVNLKPGISIYGGYSSTDWSRN
                     TTSNVTALTGPTEIGLRSSGTVTTAFRSTTVVDGLDISGGSGSTNSIAVQLDPGASPT
                     ITNNILRGKAGNVYSYGVRTSDACPCSPIISNNTILGGNAPNTNYGIYIWSATSPISI
                     NNNIISGGTAGTSQGLSLKNSTAVTISNNVIMGGSGTTNYAIYLSTSSPTVFNNTING
                     GTTGSVFAIYMTDTSNSANLRNNILFNSTGTGSCIHEAIGGMTPSAVQANNFFGCPTF
                     YQDNVTGAVTNLTTSVITGAGTNTLAFFGNTNIDNATPTAGQLFVDIDGADNNLATIA
                     DNDWHLADPGAATTCNILFGGQNLSGTLTTDRDAILRTATLPSVAPCSGNVTNGGTGW
                     SMGAYEK"
     gene            3414..>4277
                     /gene="prpF"
                     /locus_tag="PLF85_13375"
     CDS             3414..>4277
                     /gene="prpF"
                     /locus_tag="PLF85_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HNE20708.1"
                     /translation="MSSVPQVKIPATYMRGGTSKGVFFRLQDLPASCQAPGKARDALL
                     LRVIGSPDPYEKQIDGMGGATSSTSKTVIVAKSTQADHDVDYLFGQVSIDKAFVDWSG
                     NCGNLSAAVGPFAIAGGLVEKSKIPQNGLCTVKIWQANIKKTIIAHVPITNGEVQETG
                     DFELDGVTFPAAEVQLEFMDPADGEGSMFPTGNVVDDLDVPGVGKFKATLINAGIPTI
                     FLNAEDIGYKGTELQTDINSDAKALAMFETIRAHGAIKMGLIKTVEEAASRQHTPKVA
                     FVGKPTDYKSSS"
BASE COUNT         1053 a         1188 c         1099 g          937 t
ORIGIN      
        1 acggtgtcgg actcaacgcg atggcaggtt atccaatcaa actgcccggc tcttcctgct
       61 ggcctggtat tggccgctga gtcctgcacg atttcaggta cgccgactac tgcccaggcc
      121 gaggttcagt ataccattac gggcagtaat gactttggtt cgacatcgac tcaaatcggc
      181 ataaccgtct atattccacc accttcgaat cttggctaca cgggcagtcc attcacattc
      241 tcgacttaca catctgtcgg tacgatcacg ccgacactca acggtaccgt cacgagttgt
      301 acggcatcgc cagccttgcc ggcaggcctt ttgttgaatg gtaaaacctg cactatcacg
      361 ggtactgcga caacctacca gaatccggtg aatcacacca ttactgcaag caacgttctc
      421 ggcagtacca ctacaaccat caatattcgc gtagatgaat gttatgctcg cggctgcttt
      481 cgcgacaact cgaacggcac agtctctttt accggcacag gtgacggcta cgccggagtt
      541 aacctcgtgt tcatgaaatg tagccagggt caggtttgga attcattgac caatgactgc
      601 acaggtactg gcagtgctcc aaactatggc gcggccacct atgcttccag cgggggtgga
      661 tgttcgtctt taagtttcgc aggacgcagt gccggctcgt ggcgcatgcc gacaggtaat
      721 gaactagcca cgctgataca ctgtacaaac cgcgccaggg ttgcttatcc gggcagctgc
      781 ggggcaggta attatacagt gcccgccatc agtaatctat ttccgcaaac accgtcgact
      841 ggcgggactt ttccagggcg atactggtct ggtgagacat ttgttccccc cgggtcgcca
      901 ccgacgacgt tccaatatac ctcaaacttt tttgacagta cgtactctgg cgcgcggccg
      961 aattttggtg gttttcagat gcacgttcgt tgcgtggcga cggggccgta gcgcctttgc
     1021 cgcgccggct gtggtgtata cagcagtaac ggcatttgcg ttacaccccg tctgaaaaac
     1081 cgttattgtc ccgcgtcttg aaatccacca aacctgatta tagtaaaacc ctgcgcgtca
     1141 gcaactcgcc taaacacggc cgcggtgtgt acgcgaaaca accctttgcc aagggtgcca
     1201 aggtgatcga atacgtcggg aaactgatac cggtcaaaga cagcgacgcc attgccgaaa
     1261 agcagatgcg gcgtgccggc aaaagcggca cggggcatgt gtaccttttc agcctcaatg
     1321 cacgctacga catcgacggc aatgtgccct ggaatacggc gcgcctgatc aaccattcgt
     1381 gcgaccccaa ctgcgaagtg tacatcatta agggccatat ctggattctt gcaaagcgcg
     1441 cgatcgccgc cggtgaagag ctgacttacg actacgggtt caacatggcg aactggcgcg
     1501 aacacatttg ccgttgtgga acttcaaagt gcctgggcta catcgtttca gcctcgcggc
     1561 gcggggcctt ggcgagggtg ttgaaaaata ttggaaaacc gcagacctga atcagttgac
     1621 actgcactga tgcccgttcg cgtagtgtcg aatgaacaca gctcgtgcta tctatgtgct
     1681 tatcgtattc agcacagcgg gtttaatttt tctgtcgtgt tcaggatatt caaccactca
     1741 gctgttgaac gcggaatttg tcacggtcaa caaagatctg agcccaattg acttgcttgc
     1801 gccctctgac aaatctgtca gcactaaaaa cccaaaactg gtctggagca ggcgagccgg
     1861 cgcaatttct tattcggtcg aaatgtcggc tcaatctgat ttttcctcgt tgctgctaaa
     1921 aaaatcgacc acagaaactt cctatagcct gaacaatggt gatctgatta gcatcacaac
     1981 cctcgattca ttcagttact attggcgagt aaaggcaatt tacgcggacc agcaagtcac
     2041 aagtgaaagt tttcttttcc atgtgctaga cgataccata gtctatgtga gctccgcctc
     2101 aacggcaagt gaccaagtcg gtaaccgatc ggcgccattt aagaagatac agtctggaat
     2161 cgaagctgca gacaccaagc gcaatggcgt agctactacg ccgatgttcg tctatgtagc
     2221 gggtggcagt tacaccgaag aagtcaattt gaaacccggt atcagcatat acggaggata
     2281 ctcaagcact gactggtcgc gcaacaccac tagtaacgtc acggcactga ccggtccaac
     2341 cgaaattggc ctcaggagca gcggcacggt cacaaccgca tttaggtcaa ccacggtagt
     2401 cgatggtctg gatatttcag gcggctccgg cagcacaaac agcatcgcag tgcaattaga
     2461 cccaggagct tcaccgacca ttaccaataa catactgcga ggcaaagcag gcaacgtcta
     2521 ttcatatgga gtgagaacaa gcgatgcctg tccttgttcg cccataatct ctaacaatac
     2581 gatcttgggg ggtaatgccc cgaatactaa ttacggtata tatatttgga gcgccacaag
     2641 cccaatatcc attaataaca atattatttc tggtggcacc gctggtacga gccagggcct
     2701 ttctttgaaa aactctacgg cagtgaccat ttcgaataat gtcataatgg gcggctcggg
     2761 tacaacgaac tacgccatat acttgagcac ctcgtcacca acagttttta ataacacgat
     2821 caacggcggc acaacgggta gcgtttttgc catctacatg acagatactt caaatagcgc
     2881 aaacctgcga aacaatattc tcttcaatag taccggcact ggttcgtgta ttcacgaggc
     2941 cataggcggc atgactccta gcgcggtgca ggcaaacaac ttctttggtt gcccgacttt
     3001 ctaccaggac aatgttacgg gggctgtgac aaatttaacg acaagtgtga tcacgggcgc
     3061 tggcaccaac acgctggcat ttttcggcaa tacgaacatc gacaacgcaa cccccacggc
     3121 cggccagctg tttgtcgaca tcgatggtgc ggataataac ctcgcgacga tagctgataa
     3181 cgattggcat ctggcagacc caggtgccgc gacgacatgc aatattctct ttggcgggca
     3241 aaatctctcc ggcacgttaa caactgaccg cgacgccatt ttgcgcacgg ctacacttcc
     3301 ctctgttgca ccatgctctg gtaatgttac caatggtggc acgggctgga gcatgggcgc
     3361 ctacgaaaag tgattccgct ttcccgcctg tctcagagct ccgctctgca cccatgagca
     3421 gcgttcccca ggtcaaaatt cccgccacgt atatgcgcgg cggcacgagc aaaggcgtct
     3481 tctttcgcct gcaagatttg ccggcatcat gccaggcacc cggcaaggcg cgcgatgcgc
     3541 tgctgctgcg cgtgatcggc agccccgacc cgtacgaaaa gcagatcgac ggcatggggg
     3601 gagctacctc gagtacgtcg aagaccgtca tcgtggcgaa aagcacgcag gctgatcacg
     3661 acgtcgacta cctgttcggc caggtttcga tcgacaaggc attcgtcgac tggagcggca
     3721 actgcggcaa tctttcagca gcagtcgggc cattcgccat cgcgggcggg cttgtggaaa
     3781 aatcaaaaat cccacagaat ggcctgtgca ctgtgaagat ctggcaggcg aacattaaga
     3841 agacaatcat cgcacacgtg ccgatcacca acggtgaggt gcaagagacc ggggatttcg
     3901 aactcgacgg agtgaccttt cccgcggccg aagtgcagct cgagttcatg gacccggccg
     3961 atggcgaagg ttcgatgttc ccgaccggca acgtggtaga cgacctcgac gtgccgggcg
     4021 tgggcaaatt caaggccacc ctcatcaatg cgggaattcc gacgattttt ctgaacgccg
     4081 aggacatcgg ctacaaaggc accgagctgc agactgacat caacagcgac gcgaaagcgc
     4141 ttgcgatgtt cgagaccatt cgcgcgcacg gcgcgatcaa aatgggcctc atcaagaccg
     4201 tcgaagaagc cgcgagccgc caacatactc ccaaggtcgc gtttgttggt aagccgaccg
     4261 actacaagtc atccagc
//
LOCUS       DAOQJF010000287         4277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12474_length_4277_cov_5.054477, whole genome shotgun sequence.
ACCESSION   DAOQJF010000287 DAOQJF010000000
VERSION     DAOQJF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12474_length_4277_cov_5.054477"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLF85_13380"
     CDS             <1..196
                     /locus_tag="PLF85_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20709.1"
                     /translation="FKPGRITCETHFILPPKTNVCPGRVTRRIVRNNPKSTIDIVPDS
                     GHLLPFENHRLTLSLVGRYV"
     gene            226..501
                     /locus_tag="PLF85_13385"
     CDS             226..501
                     /locus_tag="PLF85_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20710.1"
                     /translation="MTEGTPMQKIFIQVLLLLVGAALMLSRNYWGKPLPVLLSGIFFM
                     NLCWITLVLGHDLKIWAWLRNNIVGNLTMIGIIVVNLIAVAVTAFIS"
     gene            508..918
                     /locus_tag="PLF85_13390"
     CDS             508..918
                     /locus_tag="PLF85_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20711.1"
                     /translation="MEILSDIQFQARLLRAVIPTLMAVLFAQSGLDKVFDFRGNLSWM
                     TDHFSKTFLRGVIPVSLVAITVLELASALLCAGGAITVLLGGSVAPAFWGMLLSAVTL
                     LALFAGQRIAKDYAGAASIALYVGIAVLGMLALH"
     gene            1186..1662
                     /locus_tag="PLF85_13395"
     CDS             1186..1662
                     /locus_tag="PLF85_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20712.1"
                     /translation="MNQASAGGILHLSSLLALNPYVLYRNREQTNNYLTEPGSSTAST
                     YNTSKDSYFGVGGFVPVYIARIEDLHIRLMPGASYAAGQTRRSQSSGTSLVYDQSANI
                     TYLSLQLFLGLQYALTRHLHVIGELGYSYSEIRASFYTDKSGNLYSGNIGLIFYFN"
     gene            1808..2863
                     /locus_tag="PLF85_13400"
     CDS             1808..2863
                     /locus_tag="PLF85_13400"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNE20713.1"
                     /translation="MTINQIEQHLGSHAGLLIHKCETIHKEALHLPSPDVVDTIYAPT
                     NRNNNVLRSLQSIIGHGRLANTGYVSILPVDQGIEHSAGASFAPNPIYFDPENIVKLA
                     IEGGCNAVASTYGVLASVSRKYAHKIPFIVKINHNEFLSYPNKFDQIMFGSVEEAAAL
                     GAVAVGATIYFGSDESARQIVEVAQAFERAHELGLATILWCYLRNSDFKKDGVDYHTS
                     ADLTSQANHLGVTIQADIIKQKLPTNNGGYNALKFGKTHAKVYSDLTTDNPIDLTRYQ
                     VANCYMGRMGLINSGGESKGATDMDEAIMTAVINKRAGGMGLISGRKAFQKPMKEGVA
                     LLNAIQDVYLCKDVTVA"
     gene            complement(2894..3355)
                     /locus_tag="PLF85_13405"
     CDS             complement(2894..3355)
                     /locus_tag="PLF85_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE20714.1"
                     /translation="MDIQKLLGSFELFKGLSEQDLALIASKAVKAEFEENQEIFHEQS
                     TESDLYILTEGRVQIIGALGKTDSATIHTILPGKLFGEFAFIDGQPRSASAVAIQKST
                     VFKIPSAALYELFDQNTKMGYTTMRNLAQILARRIRQTAHELRSSLMWERH"
     gene            complement(3359..>4277)
                     /locus_tag="PLF85_13410"
     CDS             complement(3359..>4277)
                     /locus_tag="PLF85_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20715.1"
                     /translation="VALIHFIFNTVGVLVFMPFIGQFTELMKYLSDKMEPGNISRAIA
                     NSHMFFNGINTIIFLPFVGLLEKAVRAIIKDTGPAPTVFKPQFIQPGDIKSSSLSLEQ
                     TRRELMRAGTILFETVKDLPQIFNPQNAVAAENHVETTNRQVAILSDAVKLYLRDLAK
                     LTLTNSESREATYYLHLAEDMRQLANSLHIEIMPVMKGAADKYSSFSESQKKDLDFLL
                     SKIAELLEKNLQAFEHKNIPLAEESGLLYKKLKFMSKKVQSQNLESLLAAAEQDSGAM
                     NTFVDVLDGLRVTASDVHQISKTILENQE"
BASE COUNT          914 a         1152 c         1126 g         1085 t
ORIGIN      
        1 gtttaaacct ggacgtatta cctgcgagac ccacttcatt ctaccgccga agaccaatgt
       61 gtgtccgggc cgcgttaccc gtcgcatcgt gcgtaacaat ccgaaatcca ccatcgacat
      121 tgtgcccgac agtggtcacc tgctgccgtt tgagaaccac cggcttacgc tgtcgcttgt
      181 cgggcgttat gtttgacgcc tgactcgttg ctggcagtga agtgcatgac tgaaggtacc
      241 ccgatgcaga aaatcttcat acaggtgctg cttcttcttg tgggagctgc cctcatgttg
      301 tcgcggaact actggggtaa accattgccc gtgctgctgt cgggtatttt tttcatgaac
      361 ctatgctgga ttacgctggt cctggggcat gatctgaaaa tatgggcatg gctgcgtaac
      421 aatatcgtgg gcaacctgac gatgattggt attatcgtgg taaaccttat tgctgtcgcg
      481 gtgacggcgt ttatttctta gggccctatg gaaattcttt cagacatcca gttccaggcg
      541 cggctgctgc gcgcagtgat accaacgctt atggcggtgc tctttgcaca gtcggggcta
      601 gacaaggtct ttgactttcg cggtaacctc agctggatga ccgaccactt cagtaaaacc
      661 ttcctgcgcg gtgttatacc cgtttcgctg gtcgcgatta cggtccttga actcgcatcg
      721 gcgctgctct gcgcaggggg agctatcact gttctgctgg gtggctccgt ggcgccggcg
      781 ttctggggca tgctgctcag tgcggtaacc ctgctggcac tctttgccgg gcagcgcatt
      841 gccaaagact acgccggcgc ggcgtcgatt gccttgtacg tcggtatcgc cgtactgggc
      901 atgctggccc tgcactgaac aaggccgaac gctgggctaa ttcgattttc accgtgtctt
      961 gtgctgacgc gatagcggat gagttaccga tccgaattgg caacacctta ctttagcgga
     1021 ttttcaccgc gagagagaat ctcgccgcgc gtgcgctccc taattttgtc cttgggtttg
     1081 ggtcttttct ttcttgctgg aagcgatttc ttatggggac aacaaccggc ccctgattac
     1141 ggtgcgatct ctgccaaaaa aatcggtata gacttgcgta tcggtttgaa ccaagcctct
     1201 gccggaggta ttctgcacct gtcatcttta ctggcgctga acccctacgt tctgtaccgc
     1261 aaccgcgaac agacgaacaa ctatctcact gagccaggtt cttcaaccgc atcaacttat
     1321 aatacgtcga aagactcata ttttggcgtc ggcggttttg tcccggtcta cattgcccga
     1381 attgaagacc tgcatatcag gctcatgccg ggcgcaagct acgcggcggg gcaaacacgc
     1441 cggtcacagt cgtcgggcac aagtctcgtt tacgatcaat cagcgaatat tacctacctg
     1501 agcctgcaac tttttctggg gctgcaatat gccctcaccc ggcacctgca cgtaatcgga
     1561 gaattgggtt acagttacag cgaaatccgc gcatccttct acacggataa atcgggtaat
     1621 ctgtatagcg gtaatattgg cttgattttc tacttcaact aatctccgaa atccagccag
     1681 ttcggcagct ggcggtcgat tggccatgac cgccgcaaaa aatgagaatc ctgttgtgct
     1741 ctaattgttg cgcaaggtat ttcgccttgt cgccgcgtct cgcgtccggt ttttacccgc
     1801 catggcaatg acaatcaacc aaattgaaca acacctcggt tcgcacgcgg gtctgctgat
     1861 ccacaagtgc gagactatcc ataaagaagc gctccacctg ccttcgccgg acgtggtcga
     1921 tacgatctac gctcccacca accgcaacaa caacgtgctg cgctcgctgc agtcgatcat
     1981 cggccacggc cgcctcgcca acaccggtta cgtttcgatt ctgccggttg accagggtat
     2041 cgaacactct gcaggtgctt cctttgcacc aaacccgatc tactttgacc ccgaaaacat
     2101 cgtgaaactc gcgatcgagg gcggctgtaa cgctgtggcg tcgacctacg gtgtgctcgc
     2161 gtcagtttcg cgcaagtatg ctcacaagat tcccttcatc gtgaaaatca accacaacga
     2221 atttctctct tacccgaaca aatttgacca gatcatgttt ggttcggtcg aagaagcggc
     2281 ggcgctgggt gccgttgctg tcggtgcaac catctacttt ggttcagacg agtcagcccg
     2341 ccagatcgtc gaagtggcac aggctttcga gcgcgcgcac gagctgggtc tcgcgaccat
     2401 tctctggtgc tacctgcgta acagcgactt caagaaagac ggcgtggatt accatacctc
     2461 tgccgacctg accagccagg caaaccacct cggcgtgacg atccaggctg atatcataaa
     2521 gcagaaactg ccaaccaaca atggtggtta caacgcgctg aaattcggta agacccacgc
     2581 gaaggtatat tctgacctga caactgacaa tcctattgac ctcacgcgct accaggtggc
     2641 aaactgctac atgggccgca tgggactcat taactcgggc ggcgaatcga aaggcgctac
     2701 cgacatggac gaggcaatca tgaccgcggt gatcaacaag cgtgcgggtg gcatgggcct
     2761 tatctcaggc cgcaaggctt tccagaagcc gatgaaagag ggcgtagcgc tcctgaacgc
     2821 aatccaggac gtctacctct gcaaagacgt taccgtcgct taatgaacca ttgatgcgcg
     2881 gccacgccgc gcatcaatga cgttcccaca tcaggcttga gcgcagctcg tgcgctgtct
     2941 gcctgatgcg gcgggcaaga atctgcgcca ggttacgcat cgtagtataa cccattttcg
     3001 tattctgatc gaacagttca tagagcgccg cgctcggtat cttgaacact gtcgacttct
     3061 ggattgccac ggcgctcgcc gaacggggct ggccgtcaat gaaggcaaat tctccgaaga
     3121 gttttcccgg cagaatagtg tggatggtcg cgctgtcggt tttacccagc gctccaataa
     3181 tctgcacacg cccttcggtc aggatataca ggtctgattc tgtactctgt tcgtggaaga
     3241 tttcctggtt ttcttcaaat tcagctttca cagctttcga ggcgatgagc gcaagatcct
     3301 gttcgctcag gcccttgaat agctcgaagc ttccgagtag tttctgtatg tccattgttt
     3361 attcctggtt ttctaatatg gttttgctga tctggtgtac gtcgctcgca gtcacacgca
     3421 gaccgtcgag tacatcgacg aatgtattca tagcgccgct gtcctgctct gctgcggcca
     3481 gaaggctttc gagattctgg ctttggacct ttttcgacat gaatttcagt ttcttgtaga
     3541 gcagacccga ttcttcggcc agtggtatgt tcttatgctc aaatgcctgt aggtttttct
     3601 cgagcagttc ggcaattttg ctcaggagaa aatcaagatc ttttttctga ctttcagaga
     3661 aactgctgta tttgtctgcc gcgcccttca tgaccggcat gatctcgatg tgcagcgagt
     3721 ttgcgagctg gcgcatgtct tcggcgaggt gcaggtagta ggtcgcttcg cgcgattcgc
     3781 tgttggtcag agtcagtttg gccaggtcgc gcaggtagag ttttaccgca tcggaaagta
     3841 tggccacctg cctgttcgtt gtctcaacgt ggttttctgc tgctactgcg ttctgcggat
     3901 tgaatatttg gggcagatcc ttaactgttt cgaacaggat cgtgccagcg cgcatcaatt
     3961 cgcgccgggt ctgctcgagc gaaagactgg acgacttgat atcaccgggt tgtatgaatt
     4021 gtggcttaaa gacagtgggt gccgggccgg tgtctttgat aatcgctcga acagctttct
     4081 cgagcagtcc aacgaacggc aagaagatga tggtgttgat gccgttgaag aacatgtgcg
     4141 agttcgcaat ggcgcgcgaa atattgccgg gttccatctt gtcagacagg tatttcatga
     4201 gctcagtaaa ctgcccgata aagggcataa agacaagcac gccgactgtg ttaaaaatga
     4261 aatgaatcag cgcaacg
//
LOCUS       DAOQJF010000288         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12582_length_4253_cov_4.877084, whole genome shotgun sequence.
ACCESSION   DAOQJF010000288 DAOQJF010000000
VERSION     DAOQJF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12582_length_4253_cov_4.877084"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLF85_13415"
     CDS             <1..621
                     /locus_tag="PLF85_13415"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE20716.1"
                     /translation="RLQPQRISFAVGETLATHFVPRLMVNLRALFPGVRFRVIESNLT
                     EMRAALREDKVDFALSPEPIDEGHYSNRYLLEDEIVPVVSRADLLAQRRPDWEKLRLR
                     EWILFHPGSAIRKISDRIFADIEKRFTPRVSMELRSLAGTVQCLEAGLGVGFISDLSL
                     TDRLTALRLPALTRRRRFFLSHKLRHESILPVAEAILRFAGQAPRS"
     gene            724..1128
                     /locus_tag="PLF85_13420"
     CDS             724..1128
                     /locus_tag="PLF85_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNE20717.1"
                     /translation="MPKSPFSAKELSEYKELLLEKKAHLIKEIQGKEEELTSTDKDEV
                     GDLADLATELIERELNMSISESDRARLEEIDAALERIKEKTYGICVDTGEVINKARLK
                     AVPEALRTLAAQEAYDRAQRKRKAISRVPDLD"
     gene            1129..1854
                     /locus_tag="PLF85_13425"
     CDS             1129..1854
                     /locus_tag="PLF85_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20718.1"
                     /translation="MRVDLYPELIDILRSAGKLIGSRNFFLWRGSEEPDPAGIDLSFR
                     PVIAEKVVERALDKNACVLCARRISYKKGQFEATQPVEPYLFLVHNDFLGPRAGYYNK
                     PEENTLFEKMIEGVLGFSATNLLVREILRCHFSKEDTGDTAALAHCASHLRQDIERAS
                     IRGIVLVGQAASFVFRDKAELADKQGKVFEWNGVRTIVLPGPNRLVYMREKGFPREQI
                     DAERKKIFELLTMFKEKIIGAQA"
     gene            complement(1934..2170)
                     /locus_tag="PLF85_13430"
     CDS             complement(1934..2170)
                     /locus_tag="PLF85_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20719.1"
                     /translation="MMLKKLLALLLAFTLVGGVVVAQDAEPAAETPAKEEPAKEETGK
                     KKKKAKKAKKKKAKKKKKKAKKAEEEASSEEQGG"
     gene            2424..2696
                     /locus_tag="PLF85_13435"
                     /pseudo
     CDS             2424..2696
                     /locus_tag="PLF85_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            2924..>4253
                     /gene="mutL"
                     /locus_tag="PLF85_13440"
     CDS             2924..>4253
                     /gene="mutL"
                     /locus_tag="PLF85_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNE20720.1"
                     /translation="MNPNPAQLPRIQRLDENLVAQIAAGEVIESPAAIIKELVENSLD
                     AGATEIDILIAGDGFSELQVRDNGHGIHPDDLPLAVESFATSKIRSLDELISAESMGF
                     RGEALGSIASVSRLRIESRWRHAPHAMAISVADGEKQVEPSAMDRGTRVIVRDLFYNV
                     PVRRDYFSNAAKIRRQLSETITGLTAAHPGVAFRYILNEDEPVQLAARTTLIDRLRDI
                     WGEKIGDDLLPLFYESNGMQLEGYISRFYFYRSHAGDMRFWVNQRQVQYKPLTMLLRH
                     AYGELMPKGKFPFAVLFLRIATRDVDANVHPQKREVRFRYELPVQQFMREAFTKTISA
                     EGGVSASSMVRLPQVLPASYTISSVAPAQAAENARLFDSLPPLQLADELPLPAIPENL
                     VFHSRLFNTFILASNDEGIFLIDQHTAHERINYERFLDLLADKKDISQRLV"
BASE COUNT          923 a         1132 c         1224 g          974 t
ORIGIN      
        1 cgtctgcagc cgcagcggat ctcgtttgcc gtcggcgaga cgctggcgac gcactttgta
       61 ccgcgattga tggtgaacct gcgggcgctc tttcctggcg tgcgttttcg cgtgattgaa
      121 agtaacctga cggaaatgcg ggcagcacta cgcgaagaca aggttgattt tgcgctttcg
      181 cctgagccga tcgacgaagg ccattacagc aatcgctacc tgctcgaaga cgagatcgtg
      241 ccggtagtca gccgtgccga cctgctcgcg cagcgccgcc ccgactggga gaaactgcgc
      301 ctgcgtgaat ggatactctt ccatccgggg tcggccatcc gcaagatcag cgaccgtatc
      361 ttcgccgata tcgagaagcg tttcactccc agggtcagca tggaactgcg cagccttgcg
      421 ggtaccgtgc aatgtctcga ggccgggcta ggtgttggct ttatctctga tctttcgctg
      481 accgatcgcc tgacggcgct gcgtctgccg gctctgaccc gtcgccgccg gtttttcctc
      541 tcgcacaaac tgcggcacga gagtatactt ccggtcgcag aggcaattct gcgtttcgcc
      601 gggcaggcac cacgtagctg aactcatact gaggaaaacc cgcatgaacg gagaggtatt
      661 cgctgccggt ctgcaaatac aaaaaaactt gacgctgata aaacaaaatc gtaccttcgg
      721 cgaatgccaa aaagtccctt ttccgcaaaa gaactctccg aatacaaaga gctcctgctc
      781 gaaaaaaaag cccacctgat taaagagatc cagggaaaag aggaagagct tacctctact
      841 gacaaagatg aagtcggtga ccttgctgac cttgcaaccg agctgatcga gcgcgagctc
      901 aacatgtcta tctctgaatc agaccgtgcg cgccttgaag agatcgatgc cgcgctcgag
      961 cgcatcaaag agaaaaccta cggcatctgt gtcgatacgg gtgaggttat caacaaggcc
     1021 cgcctgaagg cggtgcccga agcgctgcga acgcttgccg cgcaggaagc ttacgaccgc
     1081 gcgcaaagaa agcgcaaggc cattagccgt gtgccggacc tcgactaatt gcgtgtcgat
     1141 ctctatcctg aattaatcga tattctccgg tcagctggca agctgatcgg cagtagaaac
     1201 ttttttctct ggcggggctc tgaagaacca gatcctgcag gcattgatct ttcctttcgg
     1261 ccagttatcg cagaaaaagt cgtcgagcgg gctctcgata agaatgcctg cgtactctgt
     1321 gcccggcgga tctcgtacaa aaaggggcag tttgaggcga cgcagccggt tgaaccctat
     1381 ctttttctgg tacacaatga tttcctgggg ccgcgcgccg ggtattacaa taaacccgaa
     1441 gaaaataccc tctttgagaa gatgatcgag ggagttttgg gtttttcggc gacaaacctc
     1501 ctggtgcggg aaattctgcg ctgtcatttt agtaaagaag acacgggcga cacggctgcg
     1561 ctggcgcatt gtgcgagcca cctgcggcag gatattgaac gcgcttcgat tcgcggaatc
     1621 gtactcgtgg ggcaggcggc gagcttcgtt tttcgtgata aggccgagtt ggcagataag
     1681 cagggcaagg tctttgaatg gaatggcgtg aggacaatcg tgttgcccgg gcctaaccgt
     1741 ttggtctata tgcgtgagaa gggatttccc cgcgagcaga tcgatgcgga gcggaagaaa
     1801 atctttgaac tactgacgat gtttaaggaa aaaattatcg gggcccaagc atagagccct
     1861 gaagaccctc tacttgagcc ccgatacgtg ggagcccgga acccttgcgg gtatccgaac
     1921 gtcccaggca gaattaaccg ccttgttctt ctgaagaagc ttcttcttca gcttttttag
     1981 cctttttctt tttcttcttg gcttttttct tcttagcttt ttttgctttt ttcttcttct
     2041 tgccggtttc ttctttcgcc ggttcttctt ttgccggtgt ttcagccgct ggctcagcgt
     2101 cttgtgctac tactacgcca ccaacgagag tgaatgcgag cagtaatgct aacagttttt
     2161 tcagcatcat gttctcctaa gtctggtctt tgcgatctaa ggcgattggc ccatggagcg
     2221 cacggctcaa tggcaacctc ttttcacgcg tcaatgataa tacgttgaca ggcgggataa
     2281 tcggacaatt ttgacggcaa atccgcgggc ctgtgccggt tgctccctgg ggagcttcag
     2341 gtaaaagtcg cgcgggcgaa actttttcag agtgtcagga tgtacgtaca accgctcaaa
     2401 gggctgatca tacgcagggc gcgctgagag gaaaatcggg agtcaagaaa tcaatgaata
     2461 tctatgtcgg aaacctggcg tacaacgcca cagaagatga gctgcgttca gcgttcggag
     2521 ccttcggtca ggtgacttcg gtcaagatcg tccgtgatcg tgagacaggc cggtcgcgcg
     2581 gttttgcctt tgtcgaaatg gaagacggtg aaggtgcgca aaatgcggta gcgcagatga
     2641 atggccgcga tctcggcggt cgaaacctgg tcgttaatga agctcgccca cgcgaacaag
     2701 gcggtggtgg tggcggcggc cgtggaggat atggcggcgg cggcggtggt cgcggtggat
     2761 atggcggcgg tggtggtggc cgtggaggct acggtggcgg cggcggccgt ggtggccgcg
     2821 ggggcggtaa ctttcgccgt aaccagggcg acgacgaaac ctggtagtac cctcaacagc
     2881 gactgtttga caagggggac gcgttccccc tttttatttt atcgtgaacc caaacccggc
     2941 acagcttccc cgcatacagc ggctcgatga aaaccttgtg gcacagattg ccgccggtga
     3001 agttatcgag tcgccggcgg caattatcaa agagctcgta gaaaattcgc tcgatgcggg
     3061 cgcgaccgag atagacatac tcattgcagg tgatggattt tcagagctcc aggtgcgcga
     3121 caacggccac ggcattcatc ccgacgacct gccgctggcg gtggagagtt ttgccaccag
     3181 caagattcgc tcgctcgatg aactgatttc tgcggagtcc atgggctttc gcggtgaagc
     3241 cctcggatcg atcgcctcgg tgagccggtt gcgcattgaa tcgcgctggc gccatgctcc
     3301 gcatgcgatg gcgatttctg tcgcagacgg cgaaaagcag gttgaaccct cagccatgga
     3361 ccggggcacg cgggtgattg tccgcgatct tttttataac gtaccggtcc gccgcgatta
     3421 tttcagcaat gcggcaaaaa ttcgccgcca gctgtccgaa accattacgg ggcttacggc
     3481 ggcacacccc ggggttgctt tccgctatat cctgaatgaa gacgaaccgg tgcaacttgc
     3541 agcccgcact acgctgatcg atcgcctgcg ggatatctgg ggggagaaga ttggcgatga
     3601 cctgctgccg ctcttctacg agtccaatgg catgcagctc gaagggtaca tatcgcgctt
     3661 ctatttttac cgcagccatg cgggcgatat gcgattctgg gttaaccaga gacaggtgca
     3721 gtacaaaccc ctgacaatgc tgctgcggca cgcctacggc gaactgatgc caaagggcaa
     3781 gttccctttt gccgtgcttt tcctgcggat agcgacacgc gacgttgacg ccaacgtcca
     3841 tccgcagaag cgcgaggtgc ggtttcgtta cgagcttccc gtgcagcagt tcatgcgcga
     3901 ggcattcacg aagacaattt ctgccgaagg tggagtatct gcgtcttcga tggtcaggct
     3961 gccgcaggtt ctgccagcct cgtatacaat cagttctgtt gctccggcgc aggcggcaga
     4021 aaacgcacgc ctgtttgata gcctgccgcc gctgcagctg gcagacgaat tgccactgcc
     4081 tgcaattccg gaaaatctcg tattccattc gcggttattc aatacattta ttcttgcctc
     4141 aaatgacgaa ggcatttttc tcatcgacca gcatacggcg cacgagcgta tcaactacga
     4201 gcgattcctg gatttactcg cggataaaaa ggacatttcg cagcgcctgg tgc
//
LOCUS       DAOQJF010000289         4213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12761_length_4213_cov_4.211159, whole genome shotgun sequence.
ACCESSION   DAOQJF010000289 DAOQJF010000000
VERSION     DAOQJF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12761_length_4213_cov_4.211159"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PLF85_13445"
     CDS             complement(<1..1043)
                     /locus_tag="PLF85_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20721.1"
                     /translation="MVGMLRALPLLLVIAGCAYNQRVEAPDAQSAPSTPYVPPSPPAP
                     DTTPPGNVRSLLVTPGNGSLQISWEPPADSDCKGTRIFRADSAAAVQNAGTRTLICES
                     CSAPLAQSGLTNMTTYYYLLQSYDQAGNAAGGAITSGTPTTVAPTCADSASDNCLQIA
                     TFNLEYFTSGFDGTDSAAAQQAKRAGVASVILGNGLDLVAVQEVKDVGVFTSWVASAL
                     GDDWAYLLASSGCSARLAYVYRRSMLSALSSEELSAAPFNTTNWDGCLRRPYTATFKA
                     KHSDKQFRLINLHLKSGSDSASCSTRQPQANDINSYFNSTNSTPTLILGDFNDEVKAG
                     VGICTSLDTLNAL"
     gene            1115..>4213
                     /locus_tag="PLF85_13450"
     CDS             1115..>4213
                     /locus_tag="PLF85_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNE20722.1"
                     /translation="MLREHLQAKDIAFSLSGGVADALQALIARSESAQERELLTRGFS
                     GQEVLQHARVAPGVIVPHVRLHGLRRVVARLGIAPAGLTYDGEQIRIVLLLAVPAEAT
                     AMHLQLLQGVSSVLPAIEHALCAAGSADAVLALLHTGEEQAKPSFRNLAQQQVEFELA
                     TNVERGLSSREAAARLAEFGPNLVRRGRRIPWYVKLLRNFFSFFAILLWIAAALCYVP
                     GVDMPQLGTAILIVVIVNGFFSFLQEFKSDKAVEALKRLMANSSRVIRDGVTQEIPAD
                     ELVPGDVILVEEGDIVPADARLVEAFEIEVDNSSLTGESTSAKRYKSDQPVLLEGKFL
                     WIELPNIIFAGSTLIKGSGRAVVFATGMATEIGKIADLTLAIKSEASPLQKELRRTVL
                     AISLLAFSIGLTFLFLGWLAAGLTFAQAFIFFIGIFVANVPEGLLPTVTLSLAMGVTR
                     MAKRNAIVKNLSSVETLGCTTVICTDKTGTLTQNLMMVNEIFADGERIRIEGHGYEPR
                     GVFFRGSDSIAPEELYRNRALRKLIDCAYICNNAKLERTAHGTRIIGDPTEGCLQTLA
                     ARAGVSGIHQRVHLNPFESVRKRMSVVVKDPRTSTKTAWIKGAPLEVVSCCTKIMTGG
                     EVTDLTPDERERIHGEVDKLAAQGYRVLGFAVRDDAALQQADEYTVANTESDLVFLGL
                     TAISDPVRPGVKEAIHACHTAGIRITMITGDYPLTARSIGMQIGLGGENPVVKTGSEI
                     TFMTDAALKELLKNGETIFARVSPEQKLRIVTLLKELGEIVAVTGDGVNDGPALKKAD
                     IGISMGLRGTDVAKEAAEIILTDDNFSSIVAAIEEGRAIFANIRKFAAYVLNSNPQEM
                     YPFILWMLIPGYPLAMTVMGVLAVDVGTDLIPAMGLGVEPPEKGLMEKPPRRRQDKLL
                     SIGFILRSYLVQGSILAFSCFATYYYFAWSMGFMNGENPLMHLPASPEKLDLSKSTHI
                     YLQSLAAFFFPTIAVQIGNVLCKRSSTASLFSREFLQENVRTGLLESMRGYTPRRRSL
                     QVD"
BASE COUNT          834 a         1204 c         1282 g          893 t
ORIGIN      
        1 tcgagcgcgt tcagggtatc gagggaggtg cagatgccca cgccggcttt gacctcatcg
       61 ttaaaatcgc cgagaatcag ggtgggcgta ctgttcgtgc tattaaagta actattgatg
      121 tcgttggcct gtggctgtct tgtcgagcac gaggcgctgt ccgagccgga tttcaggtgc
      181 aggttgatca gcctgaactg tttatccgag tgtttggcct tgaaggttgc cgtataggga
      241 cggcgcaggc agccatccca gttggtggtg ttgaaggggg cggccgacag ttcttcgctg
      301 ctcaaagcgc tcagcatgct gcggcggtag acgtatgcca gccgcgcgct gcagccagag
      361 ctcgccagca ggtatgccca gtcgtcgccg agtgctgatg ccacccacga ggtgaacact
      421 cccacatctt tcacttcctg cactgcgacc aggtcgaggc cgttgccgag aatgaccgaa
      481 gccacaccgg cgcgcttcgc ctgctgcgct gctgcagagt ctgtaccgtc aaaacccgag
      541 gtgaaatatt cgaggttgaa ggtcgcgatc tgcaggcagt tgtcgctcgc gctgtcggcg
      601 caggtcggcg ccacggtcgt gggagtcccg ctcgttatcg ctccccccgc cgcattgccg
      661 gcctggtcgt agctctgcag caggtaataa taggtcgtca tattggtgag accgctctgt
      721 gccaggggtg cgctacagct ctcgcagatg agcgtgcgtg taccggcatt ctgcaccgcg
      781 gccgccgagt cagcgcgaaa aattcgcgta cccttacaat cgctgtcggc ggggggctcc
      841 cagctgatct gcagcgaacc attgccgggc gtcacgagta atgaacgtac attgccaggt
      901 ggggtggtgt caggcgccgg cggcgaaggc ggcacgtaag gcgttgaagg tgcagactgt
      961 gcatccggtg cttcgaccct ctggttatag gcgcacccgg cgataaccag gagcaaaggt
     1021 agggcgcgta gcataccgac cacggccgag accgccgctt cggcaacagg ccgcaaactc
     1081 attttcaacc cggctcgccc acgcgtcatg gcacatgctg cgcgaacacc tgcaggcaaa
     1141 agacattgca ttctcgcttt ccggcggcgt cgccgacgcg ctgcaggcgc tcatcgcacg
     1201 cagcgaaagc gcacaggagc gtgaactgct cacgcgcggt ttttccggac aggaggtgtt
     1261 gcagcatgcc agggtggctc cgggtgtcat tgtgccgcac gtgcgcctgc acggcctgcg
     1321 cagggtggtt gcccggttgg gtattgcacc cgccggcctg acgtatgacg gcgaacaaat
     1381 tcgtattgtg ttgttgctcg cagtacctgc cgaggcgacg gccatgcacc tgcagctctt
     1441 gcagggagtt tcttcggttc tgccagcaat tgaacacgcg ctatgcgcgg ccggttcagc
     1501 cgatgccgtt ctcgcgctct tgcacacggg cgaagagcag gcaaaacctt cattccgcaa
     1561 cctggcgcag caacaggtgg aatttgaact cgcaacgaac gtcgagcggg gactcagcag
     1621 ccgggaagcg gccgcgcgcc tcgctgaatt tggtcccaac ctcgtgcgcc gcggcaggcg
     1681 cattccgtgg tatgtgaagc tgttgcgtaa cttcttcagc ttcttcgcta tcctgctctg
     1741 gattgccgcc gcgctctgtt acgtcccggg cgtcgatatg ccgcagctgg gtaccgcgat
     1801 tttgatcgtg gtgatcgtga acggcttctt ttctttcttg caggagttca agtccgacaa
     1861 agcggtcgag gcgctgaaac gcctgatggc caattcttcg cgtgtcatac gcgacggtgt
     1921 cacgcaggag atccctgcgg acgagcttgt tccgggtgat gtcattttgg tagaagaagg
     1981 cgacattgtt cccgcagacg cgcgcctcgt cgaagcattc gagattgaag tcgataactc
     2041 gtcgctgacc ggtgagtcta cttctgccaa acgctacaag tctgaccagc cggtcttact
     2101 cgaaggaaag ttcctgtgga ttgagctgcc gaatattatt ttcgcgggct ctaccctgat
     2161 caagggtagt ggccgcgcag ttgtttttgc caccggcatg gcgacagaga ttggcaaaat
     2221 tgccgacctg acgctcgcca tcaagagcga agcgagcccg ctgcaaaaag aactgcgcag
     2281 gacggtgctt gccatttcac tgctggcatt cagcatcgga ctcacgttcc tgtttctcgg
     2341 ctggctcgct gcgggactga cttttgcgca ggcattcatc ttcttcattg gcatctttgt
     2401 cgccaacgtg cccgaaggcc ttctgccgac ggtgaccctt tcgctcgcga tgggtgttac
     2461 gcgtatggcc aagcgcaatg ctatcgtgaa gaacctgtct tcggttgaga ctctgggctg
     2521 cacgacagtc atctgcaccg acaaaaccgg cacgctgacg cagaacctga tgatggtgaa
     2581 cgaaattttc gctgacgggg agcgtatacg tatcgaaggg catggctacg aaccgcgcgg
     2641 cgttttcttc cggggaagcg attccatcgc ccccgaagaa ctctaccgca accgcgcgct
     2701 gcgcaagctg atcgattgcg cgtatatctg taacaatgcc aaactcgagc gcacggcgca
     2761 tggcactcgc attatcggcg acccgaccga aggctgcctg cagacactcg cggcccgagc
     2821 gggggtatcg ggtatacacc agcgtgtgca cctcaaccct tttgagtcgg tgcgcaagcg
     2881 catgagtgtc gtggtcaaag acccccgcac cagtacgaag accgcctgga tcaaaggcgc
     2941 gccgctcgaa gtggtcagtt gctgcacgaa gattatgacg ggcggcgaag ttacagatct
     3001 cacgcctgac gaacgcgagc gtatacacgg cgaagtcgat aagcttgccg ctcagggcta
     3061 ccgggtgctg ggtttcgcgg tgcgagacga tgctgcgctg cagcaggcag acgaatacac
     3121 ggtcgcaaat accgagtcag accttgtgtt cctcgggctg acggcaattt ctgacccagt
     3181 gcgcccaggg gtcaaagaag cgattcatgc ctgccacacc gccggcatac gcatcacgat
     3241 gatcacgggc gactacccgc tgacagcgcg cagcatcggt atgcagattg gtctcggcgg
     3301 tgaaaacccc gtggtgaaga ccggcagcga aatcacgttt atgaccgacg ccgcgctcaa
     3361 ggaactcctg aaaaacggcg aaaccatttt tgcgcgcgtg tcgcccgagc agaagctccg
     3421 cattgtgacg ctgctcaaag agctgggtga aattgtggcc gtgacgggtg acggtgtcaa
     3481 tgacgggcca gcattaaaaa aagcagacat cggtatttcg atgggcctgc gtggcaccga
     3541 cgtcgcgaaa gaagcagcgg aaatcattct caccgatgac aacttcagtt cgattgttgc
     3601 agcgatcgaa gagggccggg cgatattcgc gaatatccgc aagtttgccg cttacgtgct
     3661 gaacagcaac ccgcaggaga tgtacccgtt tattctctgg atgctgattc ccgggtaccc
     3721 gctcgcgatg acagtgatgg gagttctcgc ggtggacgtg ggtaccgacc tgattccggc
     3781 catgggactc ggggttgagc cgccggagaa agggctcatg gaaaaaccgc cgcggcgccg
     3841 gcaggacaaa ctgctttcga tcggttttat tttgcggtcg taccttgtgc aaggcagtat
     3901 tctcgcgttc agctgttttg ccacatacta ttattttgcc tggtcgatgg ggtttatgaa
     3961 cggagaaaat ccgctgatgc acctgccagc ttcgccggaa aagctcgatc tcagcaagtc
     4021 gacgcacatc tacctgcagt cgctggctgc attctttttc ccgacgatcg ccgtgcagat
     4081 cggtaacgtg ttgtgcaaac gttcatcaac cgcgtcgctt ttctcgcgcg agttcttgca
     4141 ggagaatgtg cgtaccgggt tgctcgagag catgcgcggt tatacacccc gccggcgctc
     4201 gctgcaggtc gat
//
LOCUS       DAOQJF010000290         4210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12778_length_4210_cov_5.031528, whole genome shotgun sequence.
ACCESSION   DAOQJF010000290 DAOQJF010000000
VERSION     DAOQJF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12778_length_4210_cov_5.031528"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..712)
                     /locus_tag="PLF85_13455"
     CDS             complement(203..712)
                     /locus_tag="PLF85_13455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE20723.1"
                     /translation="MRDPRDSIHILDFGCGHGYVAMVLATLAHPGLRVYACDSDEECL
                     DVLWGRIAHRQVQNLTAFHLPNYSQVYLPGWLPAPDYVVCSFSISALEHPDIGLPQLV
                     RQVPSGTNFLFVEWDPERSHKLIDIYVPAARRITPADFRRLVEYSGLKLTSEESGKQL
                     YYSVKAVKP"
     gene            complement(809..1105)
                     /gene="gatC"
                     /locus_tag="PLF85_13460"
     CDS             complement(809..1105)
                     /gene="gatC"
                     /locus_tag="PLF85_13460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNE20724.1"
                     /translation="MSISTDEVKKIASLSKLRLEGSELEKMAHDFNGILDFVAQIKEA
                     DTSGAHVLDHPLGHTNSSREDVSEPSIGQDAIRAFAPKFEAGFFVVPRVIETEG"
     gene            complement(1146..1826)
                     /locus_tag="PLF85_13465"
     CDS             complement(1146..1826)
                     /locus_tag="PLF85_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20725.1"
                     /translation="MDYTNIDQNFFLKTLPALVANAALVLYALARRPPLWRYLTLFGI
                     TTLADISVAGQLVPIASAEMQTGIEYIFVLIGDLRFILLLAYILYAGKTLADINALRL
                     TGDVLRPALIFTLFPTLIVSAIGFAKPELLQLPRHKFLAYELVFFGLTLLWLKVVLPQ
                     KPVAEPEQRFIRQVALPVLGFYGLWSLADILILRGMQVGHAVRILPNLLYYCLFLWWV
                     AFAARQKA"
     gene            complement(1830..2867)
                     /locus_tag="PLF85_13470"
     CDS             complement(1830..2867)
                     /locus_tag="PLF85_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE20726.1"
                     /translation="MLKHRMREYLSFFWRRLSQGLSSERLTYTFLFLFVFTGAFAPFL
                     ASSQPLFIYDKAGYESKSGTALKRSPLLYTLFYNDTQVEKIEYRRLVEKGEIGAIFTL
                     VPYSPYETELENYLKPPRFLGARFVHYLGTDENGRDVAARLIRGTRNSLLVSLVAVGL
                     ALAIGVFVGALAGYFGGWVDMVISRFIEIVICFPKLILIIAVMALKKPSLLNLMIVIG
                     LTSWTGIARLVRGEVMRVRNFDYVQSARAMGASSWRIIFIHILPATAGPLSVTAAFDV
                     AEAVLTESALSFLGIGVPIPEPSWGDVLKTAQDFPDIAWWMTLFPGLLIFLTVITFNN
                     LGDKLRQKFAR"
     gene            complement(2870..3874)
                     /locus_tag="PLF85_13475"
     CDS             complement(2870..3874)
                     /locus_tag="PLF85_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE20727.1"
                     /translation="MRYALRRILLMVPTFIGIITITFFLIKLRPDALTTASMGPDGMK
                     ESAGLDEFQKKMRAYYGLDDPMHVQYLRLWKNIVTLNFSESRIDHRPVLDKIAEALPV
                     TLFFNLLTVFLVYTISIPLGIYMAIHDQSRREKILASVLYVLYALPAFWVSLMLLKYL
                     ASAEYLDLFPLSGLVSPFFDKLAWYQKIGDFLWHLVLPVTVMVYGSFAFLSRYMKSSF
                     MEALRSDYVRTARAKGLSGATIIYVHALRNSVIPLVTLLGGLLPSLIGGSVILERIFS
                     IPGMGKLAYDSFYANDDTVIIAIVSISSVLTMLGIFLSDIAYMLVDPRIRYGVQSAEA
                     "
     gene            4005..>4210
                     /locus_tag="PLF85_13480"
     CDS             4005..>4210
                     /locus_tag="PLF85_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20728.1"
                     /translation="MRKLLRYLILLLLVAASAVAAVPWYMAYRAREQIFAATAGNPPP
                     HPAQVALIPGASVLPNKKPSATLLG"
BASE COUNT         1015 a         1106 c         1247 g          842 t
ORIGIN      
        1 ggttcgacaa gctcaccacc agcaagcagg gccgcctgaa aggcgggcgc aacacggttg
       61 cgcggtcgca ggttcgttcg cgagccggta ggcgagcgcg ccatcttgca tcgcccacac
      121 ttccatacta tagtcacatc tggttagagc acctgcctta cagatcgaca agctcactgc
      181 aggcaagcag ggctgcccaa ttctacggtt ttactgcttt gactgaataa tagagctgtt
      241 tgccgctttc ttctgaggta agtttcagtc cgctgtattc caccaggcgg cggaagtctg
      301 ccggtgtaat gcggcgtgcg gcggggacgt agatgtcgat aagtttgtgc gaccgttcag
      361 gatcccattc gacaaacagg aaattggtgc ccgaaggcac ctgcctgaca agttgcggca
      421 gtccaatatc aggatgttcg agtgcagaaa tactgaaaga gcaaacgacg tagtccggag
      481 cgggcaacca gccgggcagg taaacctgcg agtagttggg caggtgaaag gccgtcaggt
      541 tttgtacctg ccggtgtgct atgcgacccc agagtacgtc caggcattct tcgtctgagt
      601 cgcatgcata aacgcgcagg ccaggatgtg ccagggtcgc gagcaccatc gcaacgtagc
      661 cgtgcccgca gccaaaatca agtatgtgta tagaatcgcg cgggtcgcgc atgatctgcg
      721 cagtgtagag ctgcgcggga ataagtttat ctctctggtc agagaccagg aatttctggt
      781 attcggtaaa ttcgtccgcg tgttgcagct agccctcggt ttcgatgacc cgcggtacaa
      841 caaagaagcc ggcttcaaac ttcggtgcga aggcacgaat cgcgtcctgg cctatcgacg
      901 gctcgctgac atcttcacgc gacgagtttg tatgccccag cgggtggtcg agtacatggg
      961 caccactcgt atcagcttct ttgatctgtg cgacgaaatc gagaatgccg ttaaagtcat
     1021 gcgccatttt ctcgagctcg ctgccctcga ggcggagctt gctgagcgag gcgatctttt
     1081 tcacttcgtc ggtgctgatg gacatgggta aagatccacc cggatactca gggcgaaaag
     1141 aagaatcagg ccttctgccg ggcggcaaaa gccacccacc acaggaacag gcaatagtag
     1201 agcaggttgg gcagaatgcg cactgcatgg ccgacctgca taccgcggag tatcaggatg
     1261 tcggccagcg accagagacc gtagaagccc agcacgggca gtgcaacctg ccggataaag
     1321 cgctgctctg gttcggcaac gggtttttgc gggagtacca ctttcagcca gagcagggtg
     1381 aggccgaaga agaccagctc gtacgccagg aacttgtgcc tcggcagctg cagcagctca
     1441 ggtttagcaa aaccaatagc agaaacaatc aacgtcggga acagggtgaa gatcagtgcg
     1501 ggccgcagga catcacccgt caggcggagc gcattgatgt ccgcgagcgt tttgcccgca
     1561 taaagtatat aggcgagcag cagtatgaac cttagatcgc cgatgagcac gaagatgtac
     1621 tcgatacccg tctgcatctc tgcgctggct attgggacca gctgcccggc gacagagatg
     1681 tctgccagtg tcgtgatgcc gaaaagagtg aggtagcgcc agaggggtgg tctgcgcgcc
     1741 agtgcataga gcaccaatgc agcattggcc accagtgccg gcagggtttt aaggaaaaaa
     1801 ttctggtcga tattcgtgta gtccatggtc tagcgtgcga acttctggcg caacttgtct
     1861 cccaaattgt taaaagttat cacggttaga aaaatcagca gtcccgggaa aagcgtcatc
     1921 caccaggcga tgtcgggaaa gtcctgcgcg gttttcagca catcgcccca cgagggttcg
     1981 ggaatcggta cgccgatgcc cagaaagctg agagccgatt cggtcagaac ggcctcggcc
     2041 acatcaaagg cggccgtgac tgacagtggg cctgcggtcg ccggcagtat gtggataaaa
     2101 ataatgcgcc atgacgaagc tcccattgcg cgcgcgcttt gcacgtagtc gaagttgcgc
     2161 acgcgcatga cttcaccgcg cacgaggcgt gcaataccgg tccagctggt gagcccgatc
     2221 acaatcatca ggttgagcag tgaaggtttc tttagcgcca tgaccgcgat aatcaggatc
     2281 agcttaggaa aacagatgac gatttcgatg aagcggctga taaccatatc gacccagccg
     2341 ccgaagtaac ctgccagcgc accgacaaaa actccgatcg cgagcgccag gcccactgcc
     2401 accagcgaga cgagcagtga attgcgggtg ccccgaatga ggcgggcggc gacgtcacgg
     2461 ccattctcat cggtaccgag gtagtgcaca aaccgggcgc ccaaaaaccg tggcggtttg
     2521 aggtagtttt cgagctcggt ttcataaggt gaatacggta cgagtgtgaa gattgccccg
     2581 atctcgccct tttctaccag gcgccggtat tcgatttttt cgacctgtgt gtcgttgtag
     2641 aagagcgtat agagcagcgg tgacctcttc agtgctgtgc cggatttact ttcgtaaccg
     2701 gccttgtcgt agatgaagag gggctgcgac gaagcgagaa aaggcgcaaa cgctcccgtg
     2761 aatacaaaaa gaaacaggaa ggtgtaggtc agcctttcgg agctaaggcc ctgcgacagc
     2821 ctgcgccaaa aaaaactgag atattcgcgc attctgtgct tgagcatgat caggcctcgg
     2881 cgctctgcac gccgtaacgt atgcgggggt cgaccagcat gtaggcgatg tcactcagga
     2941 agatacccag catcgtcagg accgaagaaa ttgagacgat tgcaatgatt acggtatcgt
     3001 cgttcgcgta aaaactgtcg taagcgagtt tgcccattcc cggtatagag aagatacgtt
     3061 cgagaatgac cgaaccgccg atgagagaag gcaacaagcc accgagcagc gtcacgagtg
     3121 gaatcaccga attgcgcagt gcgtgtacat aaatgatggt agctcccgaa aggcccttag
     3181 ccctggctgt gcgcacatag tccgagcgca gcgcttccat aaaagaagat ttcatatagc
     3241 ggctgagaaa ggcgaacgaa ccgtagacca ttacggtcac gggcaatacc aggtgccaga
     3301 gaaaatcccc gattttctgg taccatgcca gcttgtcgaa aaacgggctg acgaggccgg
     3361 aaagcggaaa gaggtccagg tactccgccg aagcgaggta cttgagcagc atcagagaaa
     3421 cccagaacgc cggcagcgcg tagagcacat agagcaccga agcgagaatt ttttcccgcc
     3481 gcgactggtc atgaattgcc atgtagatac cgagcggaat cgagatagta tacacgagga
     3541 agacagtcag caggttaaaa aacagggtca ccggcagagc ttcggcaatc ttgtcgagca
     3601 cgggacggtg gtcgatgcgg ctttcggaga aattgagggt cacgatattt ttccatagcc
     3661 gcaggtactg cacgtgcatc gggtcgtcga gaccatagta cgcgcgcatt tttttctgga
     3721 attcgtcgag gccggcactc tctttcatgc cgtcaggccc catcgaggca gtggtcaatg
     3781 cgtccgggcg caacttgatc aggaaaaaag ttatggtaat gataccgata aaagtcggca
     3841 ccatcaggag tattcgccgt agagcgtagc gcacgtgcat gcgatgacca tgactcgaga
     3901 acgacggtca agtataatgg ggctgcgacc tgccgcgtgg ctcacgttac gctacagaat
     3961 acggttttca ggctgcgggg caaattttac gaggattgct ttacgtgcgc aaattgctcc
     4021 gctacctcat tttgctgctg ctcgtcgccg cgtcggcggt tgccgcggtg ccgtggtata
     4081 tggcgtaccg cgcgcgtgag cagatttttg ccgctacagc gggtaatccg ccaccgcacc
     4141 cggcgcaggt cgcgctgatt cccggggcga gcgtactgcc gaacaagaaa ccctccgcca
     4201 cgctgttggg
//
LOCUS       DAOQJF010000291         4207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12792_length_4207_cov_4.134152, whole genome shotgun sequence.
ACCESSION   DAOQJF010000291 DAOQJF010000000
VERSION     DAOQJF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12792_length_4207_cov_4.134152"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..688)
                     /gene="msrA"
                     /locus_tag="PLF85_13485"
     CDS             complement(182..688)
                     /gene="msrA"
                     /locus_tag="PLF85_13485"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNE20729.1"
                     /translation="MIRKAVLAGGCFWGLEELLRSQPGVLDTECGYCGGSVENPTYRN
                     HEGHAEAVEITYDADKSSFRNLLNFFFQIHNPTTMNRQGNDIGTSYRSTIFYANEEEK
                     REAEDFIRIVNESKRWQKPVVTTLEPLGEEHGAAGKFWPAEDYHQDYLQKNPNGYTCH
                     RIYFNSYI"
     gene            740..1537
                     /locus_tag="PLF85_13490"
     CDS             740..1537
                     /locus_tag="PLF85_13490"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNE20730.1"
                     /translation="MELRFNILAAVLLVTPLSAQIFNVEKVRLDHPEDRVIFGNIGAN
                     FTYHNRSANLQTPVRVLNGGLTSDVGYASDSHLYLLINNYQVLNVNDSYVVNFGSTHF
                     RTQFFHRNTLSVESYAQYQYDQARGLELRLLAGAGPRWRIIKTPAWNLATGTSLMYEG
                     EDWQNPAIAGDPILQARYVKSSTYISTRYVVNPQVDVNAVTYYQVGYDREFDATLHRL
                     SGEANLAVQLFGNLSFTTSFTAAWESRPIVPIVPFIFMVTNGVRYRF"
     gene            complement(1544..2194)
                     /locus_tag="PLF85_13495"
     CDS             complement(1544..2194)
                     /locus_tag="PLF85_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20731.1"
                     /translation="MKKRMFGRALLLCALLSPLPAQEANAEAGAAETPTESTYQKKPV
                     IAEWEGNGAIYAGEAVEEKDGKILIHWDGGNPAPSWVDAEKVYPSLAELLHQKKKLRE
                     VYAMGSNGYYYRGLVLFERNGKSLIHFEAGGRPEWVENKTLKPRKGHGLKANLIADRE
                     LTPAEKAKQAKADAQFAKERAAKYPGICGAIATQVDCLRTYDPCTWTGGRCRYRGY"
     gene            complement(2227..2823)
                     /locus_tag="PLF85_13500"
     CDS             complement(2227..2823)
                     /locus_tag="PLF85_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20732.1"
                     /translation="MQGFSERSEPLSASDREQLRGRFHTALAIFFLLLLPLLTIFAYW
                     LWIFFDSARRDPHGSGFVFLVVAFATLLWLFITGALLYWLRARYRFASLQHKTVYSGT
                     VTDSWRRAGNASSLYSSHSRYVLRLGETDFDVDPDIQKFVPVGVQIELHCITPRREVL
                     YHVELSAERRRAWEELLAQPRESVFRQIWSLILAFVFR"
     gene            complement(2837..3199)
                     /locus_tag="PLF85_13505"
     CDS             complement(2837..3199)
                     /locus_tag="PLF85_13505"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HNE20733.1"
                     /translation="MKLNPLTEEEARVILHKGTEYPGTGEFNKHYAKGTYLCKQCEAE
                     LYRSDDKFDSGCGWPSFDDEVPGAVKRIPDADGRRTEIVCAQCDGHLGHVFLGERFTA
                     KNTRHCVNSISLLFRPAK"
     gene            3350..>4207
                     /locus_tag="PLF85_13510"
     CDS             3350..>4207
                     /locus_tag="PLF85_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE20734.1"
                     /translation="MSLVSLLKGKGKSGFGYGSTAEEVTFGLDLTGKNILITGCNSGL
                     GLETARVLALRGARILGTARSLEKAEGALIDFGSIHKAYECELAEPRSVKACVTAIKK
                     DRIKLDAIICNAGIMALPTLQKSHGYELQFFTNHIGHFILVTGLLDTMHDDGRIVMLS
                     SAAHNMAPKVGIEFDNLSGDVGYKPWKAYGQSKLANLLFAKELSRRLAGSGKTANAVH
                     PGVIRTNLGRHMPAIARVVFAGVGPLFLKSVEQGAATQCYVAVHPELAKNSGAYFADV
                     NIASPRIDAS"
BASE COUNT          916 a         1179 c         1168 g          944 t
ORIGIN      
        1 ggccctcgag cagtatcggt atggcctttt cctgaatcgg cgaagcctcg cgaaaaccca
       61 tatcggttat cgcctgcaca agtgtaggcg ataaccctag ttcataaaat gtttttttca
      121 tggagttatt ggccgaatca gatccgctgg cgcagccagc ggatctgatt cggcgggaaa
      181 tctagatgta gctattaaaa taaatgcgat ggcacgtata gccatttggg ttcttctgta
      241 aatagtcctg gtggtagtct tctgccggcc agaacttgcc cgcggcgcca tgctcttcac
      301 ccagcggctc aagcgtcgtc acgacgggtt tctgccagcg cttcgattca ttcacgatgc
      361 gaatgaaatc ctccgcttcg cgcttctctt cttcatttgc gtagaagatc gtggaacggt
      421 aagaggtgcc tatgtcatta ccctggcggt tcatcgtcgt ggggttgtgt atctggaaga
      481 aaaagttgag caggttgcga aacgagctct tgtcggcgtc gtacgtaatt tctacggcct
      541 cggcatgccc ttcatggttg cggtaggtgg gattttctac actgccgccg cagtagccgc
      601 attcagtgtc gagcacgccg ggctgtgaac gcaaaagctc ttcgagcccc cagaagcaac
      661 cgccagcgag cactgccttt ctgatcatga acagaatata acgtttttcg ctgcggcagc
      721 aactgaaaat tcggaccgaa tggagctacg gttcaatatc ctcgccgcag tgctgctagt
      781 aaccccactg agcgcgcaga tctttaacgt cgaaaaggtg cggctcgacc accctgaaga
      841 ccgcgtgatc ttcggtaaca tcggcgctaa cttcacgtac cataaccgca gcgcgaacct
      901 gcagacgccc gtgcgcgtgc tcaacggtgg cctgacttct gacgtcggtt atgccagcga
      961 cagccacctg tacctgctca tcaacaacta ccaggtgctc aatgtgaatg acagctatgt
     1021 cgtgaatttc ggctcgacgc attttcgcac gcagtttttt catcgcaata cgctgagcgt
     1081 cgagtcatac gcccagtacc agtacgacca ggcacgcggg ttagagttgc ggttgctggc
     1141 aggcgccggg ccccgctggc gcattattaa aacccccgcc tggaatctcg cgacgggaac
     1201 gagcctcatg tatgaaggtg aagactggca gaacccggcg attgcaggcg accctatctt
     1261 gcaggcgcgc tacgtgaaat cgtcgaccta catcagtaca cgctacgtag tgaacccaca
     1321 ggtcgacgtc aacgcggtga cttactacca ggtcggttac gaccgcgaat tcgatgcgac
     1381 actgcaccgg ttgagcggtg aagccaacct ggcagtgcag ctattcggca acctgtcctt
     1441 tacgacatct ttcacggccg cctgggagtc acggcccatc gtgccgatcg tcccctttat
     1501 ctttatggtg acgaacggcg tgcgatatcg gttctagatt aaatcaatac ccgcggtaac
     1561 ggcagcgacc acctgtccag gtacaggggt cgtacgtgcg caggcaatcg acctgtgtgg
     1621 caatggctcc gcagatgccg gggtatttag ccgcccgctc cttcgcgaat tgcgcatcag
     1681 ctttcgcctg cttcgctttt tcggcaggag tgagttcgcg gtcggcaatc aggtttgcct
     1741 tgagtccatg ccccttacgc ggtttgagtg tcttgttttc tacccactcg ggccggccac
     1801 ccgcttcgaa atggatcagg ctcttgccgt tacgttcgaa cagcaccagg ccacggtagt
     1861 aataaccgtt actgcccatc gcgtagacct cacgcaactt cttcttttgg tgcagcagct
     1921 cggcaaggct tgggtatacc ttttccgcat ctacccatga cggtgccgga ttgccaccgt
     1981 cccaatgaat gagtatcttg ccgtcttttt cttcgacggc ttcaccggcg tagatcgcgc
     2041 cgttgccttc ccattccgcg attactggtt ttttttgata ggtactttcg gtcggagttt
     2101 ccgctgcgcc ggcttcggca ttagcttctt gtgcgggcag gggactgagc agtgcacaca
     2161 ataaaagtgc acgaccgaac attctttttt tcattgcaag ctccttgctg atggcggcgt
     2221 gcgcaatcag cgaaatacaa aagccagaat cagtgaccag atttgcctga aaacactttc
     2281 tctgggctgc gcgagtaatt cttcccaggc gcgcctgcgc tcggcagaga gctcgacatg
     2341 gtagagcacc tcgcgccggg gcgtgataca atgcagctca atctgcactc ccacaggcac
     2401 aaacttctgg atatcggggt cgacgtcgaa gtcggtctca ccgaggcgta gcacgtacct
     2461 ggaatgcgac gagtaaagcg aactggcgtt acctgccctg cgccagctat cggtcaccgt
     2521 gccggagtac actgtcttgt gctgcaatga ggcgaagcgg tagcgcgcac gcagccagta
     2581 gagcaaagcc cccgtaatga agagccagag caacgttgca aatgccacca ccagaaaaac
     2641 gaagccgctg ccgtgcgggt cacgccgcgc agaatcgaag aaaatccaca gccagtaagc
     2701 aaagatggtg agcagcggca aaagcagcag aaagaagata gccagcgcag tgtgaaaacg
     2761 gccgcgcagc tgctcgcggt ctgaggctga aagtggttcg ctgcgctcgc tgaaaccctg
     2821 catgaccagc cgaatgctat tttgccgggc gaaagagaag cgaaattgag ttcacgcagt
     2881 ggcgggtatt ctttgccgta aagcgttcgc ccaaaaacac atggccaaga tggccatcac
     2941 actgtgcgca gacaatttcg gtgcggcggc catcggcgtc aggtatcctt ttgaccgcac
     3001 cgggaacttc gtcgtcaaaa ctcggccagc cgcagcccga atcgaacttg tcgtcagaac
     3061 ggtagagctc cgcctcgcac tgcttgcaga ggtacgtgcc ctttgcgtaa tgcttgttga
     3121 attcgccggt gccggggtac tcggtgccct tgtgcagaat gactcgggct tcttcttcgg
     3181 tgagggggtt gagtttcata cgctttgagc aagaagataa aatacgctgc gcggagcgac
     3241 ttttttttgg tccggtcttt acggcatccg gctgtacacg ccatcggggc aaagcaggtt
     3301 acacaggcct cgcgagtcgc gggcaatagg tacataacgg gagtcacata tgtcactggt
     3361 atcattactc aaaggcaaag gaaaatccgg ttttggttat ggctcgaccg ctgaagaggt
     3421 cacgtttggc ctcgatctca caggcaagaa catcctgatc acgggatgca attcgggtct
     3481 cggtctcgaa actgcgcgcg tgctggcgct gcgcggtgcc cgcattttgg gaacggcacg
     3541 ttcgctcgaa aaagcagagg gagcgctcat tgatttcggc agcatacata aggcgtatga
     3601 atgcgaactg gctgagccgc gttcggtcaa ggcctgtgtc accgcaataa agaaagaccg
     3661 catcaagctc gatgcgatta tctgcaacgc gggaatcatg gcgctgccaa ccctgcagaa
     3721 gtcgcacggt tatgagctgc agtttttcac caaccatatc ggtcacttta tactcgtcac
     3781 tggtctgctg gataccatgc atgacgatgg ccgtatcgtg atgctttcga gcgccgcgca
     3841 taacatggca cccaaagtgg gcatcgaatt cgacaacctt tcgggtgacg tcggctataa
     3901 accgtggaaa gcctatggac agtcaaagct ggctaatctg ctgttcgcca aagaactctc
     3961 gcgccggctt gccggcagcg gcaaaacagc caacgcggtg cacccgggcg tcattcgcac
     4021 aaacctcggt cgccacatgc cggcaatcgc ccgggtggtc tttgccggtg tcggcccgct
     4081 gtttttgaag tcagtcgagc agggtgcggc gacgcagtgt tacgtggcgg tgcatccaga
     4141 gcttgccaaa aactctggcg cgtactttgc cgatgtcaac attgcgagcc cgcgcatcga
     4201 cgcctcg
//
LOCUS       DAOQJF010000292         4206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12800_length_4206_cov_3.905083, whole genome shotgun sequence.
ACCESSION   DAOQJF010000292 DAOQJF010000000
VERSION     DAOQJF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12800_length_4206_cov_3.905083"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="PLF85_13515"
     CDS             <1..1581
                     /locus_tag="PLF85_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE20735.1"
                     /translation="ISLLNIEDAIARVQELIDEIFTDTHSIVLYWNDAAGAFIPVKEA
                     DRTEQLKFRIFDDFMLWLGEQDRIFSRHDFTHGRKFAHIREPALEFIKTTGAEILVPF
                     NLNKSVLALLYLVGREGGRQYTKKELRFLLEIRDITTVSLSNAALYERLHAMLFHLEE
                     KVKERTSELSNAQAQLIQQEKMASLGVMVAGIAHEINTPTSVVSGSAENLNNNLQHII
                     TQIVSDAIPPEPMRKLLVQAQRVRDLKTGEPLSAIAKLRAGRELVPRLTAPGIIDENE
                     ALARADLLMDLGLNRDEEIINLTAQSSQPEFQVFRAVVNIDKNLRNMRFALSSILSIV
                     KALKHYSHRDQAEAENIDVREGVENTLTVFHNQLKHGILVNRHYEQVSKITCNPGELN
                     QIYSNLLVNSVHAMKGKGSIDIFIRERSIDAVLDSKNVHDDKKLETLLGDKPQNRYVL
                     ITLQDSGPGIPAEYQIKIFDPFFTTKAPGEGTGLGLGIIRNVVLKHNGLLAFSSQPGC
                     TRFTIALPVEHVPPGEAE"
     gene            complement(1614..3293)
                     /locus_tag="PLF85_13520"
     CDS             complement(1614..3293)
                     /locus_tag="PLF85_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HNE20736.1"
                     /translation="MSKQTTELSEVEGVKEASNFLRGNIARDLVNGTDKFEEEEKQLL
                     KFHGLYQQKDRDKKAEGEPEKRNTFMLRGRIPGGRLTAEQYLAWDYLGERYGGGALRL
                     TTRQSVQLHGLLKEDLKAVIQEIHRVGLTSMGACGDVVRNVTEALNITGNPLYNQLSF
                     FSQQLSDYFKFETTAYVEIWLDDVQQNPVQQERIYGKTYLPRKFKIAVCLAGENGVDL
                     YANDMGFAATHTNGKIDGFFVFAGGGGGMTHNKPETFPRAADLLGWIPVDALLPVATA
                     IVTAHRDWGDRTNRKHARLKYVLADRGVEWFRTEIEQRSQVKLNTTRPLPPWKIPEHH
                     GFIRKPDGTYNFGLYIMNGRIKDIPGYRLKTAIQLVVARTKCALQVTPDQDLLFLDLD
                     EKQRHELVHIFRAHGINTNRPNPLYRRALACVSLPTCALALTESERYFPHVLRDINRV
                     VEKHGLMDRAPLVRMTGCPNGCARPYSAEIGIVGQQNGGKYAIFLGGNHLGTRVGEAV
                     LQKVQMQELPGYLDKAFAFWAAESNAGERFGDFVNRVGLDQIKAVLAPPEE"
     gene            complement(3274..3891)
                     /locus_tag="PLF85_13525"
     CDS             complement(3274..3891)
                     /locus_tag="PLF85_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNE20737.1"
                     /translation="MLLPLSFDLDGREILLVGGGKAALEKYVQLRRTACRLAIVAREL
                     KSDFHEALQAATLASVTIHERPFTPQDIEGKFIVFSAVDDEAVAEQIFQLCRQKQILI
                     NSADDRARCDFFTNALIDRGPVTLSVSTQGRFAGFSAILRRHLEEVLPPELDDDWARV
                     FDLRKRAIALQSVSEKKSVITNIVRDIEQKFFRSERGQKHEQADH"
     gene            complement(3869..>4206)
                     /locus_tag="PLF85_13530"
     CDS             complement(3869..>4206)
                     /locus_tag="PLF85_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20738.1"
                     /translation="IWITDGHSEDVARHAASMATFPGTLVFYMGAGRSVEIAQLLISN
                     GIDESKPCVLVENAGSEHRHVSRGSVADAAAGAISRSTHGPGILLLGDALAAETAYTE
                     ADYAAATQL"
BASE COUNT          910 a         1196 c         1177 g          923 t
ORIGIN      
        1 atttcgctgc tcaacatcga agatgctatt gcgcgcgtgc aggagctgat cgacgaaatt
       61 tttaccgaca cgcattcgat cgtcctctac tggaacgacg ccgcgggagc tttcattccg
      121 gttaaagaag ccgaccgtac cgagcagctg aaattccgca tctttgacga ctttatgctc
      181 tggctgggcg agcaggaccg cattttcagc cggcacgatt tcacccacgg gcgcaagttt
      241 gctcacattc gcgagccggc gcttgaattc ataaaaacta cgggagcaga gattctcgtt
      301 ccttttaatc ttaataagtc ggtactcgcg ctcctctacc ttgtaggccg cgaaggtggg
      361 cggcagtaca cgaagaagga gctccgcttc ctgttggaaa tccgcgacat cacgacggtc
      421 tcgctgtcta acgccgcgct gtacgaacgc ctgcacgcga tgctctttca tcttgaagaa
      481 aaggtgaaag aacggacgag cgagctctcc aatgcgcagg cacagctgat acagcaggaa
      541 aagatggcga gtctcggggt catggttgcc ggtatcgcgc acgagatcaa tacaccgaca
      601 tcggtcgtca gcggctcggc agaaaacctc aacaacaacc tccagcatat tattacgcag
      661 attgtcagcg acgccatccc gcctgaaccg atgcgcaagc tactggtgca ggcacagcgc
      721 gtgcgcgatc ttaagaccgg tgagccgctt tcggcgattg ccaagttacg ggccgggcgt
      781 gagctggtgc cgcgcctcac tgcaccgggc atcatcgatg aaaacgaagc tctcgcacgc
      841 gccgacctgc tgatggacct ggggctcaac cgggacgaag aaatcatcaa cctgaccgcg
      901 caatcctctc agccggagtt ccaggttttt cgcgcagtgg taaacatcga caagaacctg
      961 cgcaatatgc gttttgcgct ctcgtcgatt ctcagcatcg tgaaggcatt gaagcactat
     1021 tcgcaccgcg accaggccga agcggaaaat atcgatgtga gagaaggtgt cgaaaatacg
     1081 ctgacggtct tccacaacca gttgaagcat ggtatactgg tgaaccggca ctacgagcag
     1141 gtgagcaaaa tcacctgcaa tccgggcgaa ctcaaccaga tctacagcaa cctgctggtg
     1201 aattccgtgc acgctatgaa gggtaaaggc tcaatcgata tcttcattcg cgagcgatcg
     1261 atcgatgcgg tgctcgattc aaaaaatgtg cacgatgaca agaaactcga aaccctgctg
     1321 ggtgacaagc cgcagaacag gtatgtactg attacgctgc aggattcagg gcccggtata
     1381 ccagccgaat accagatcaa gatctttgac ccgttcttca ccaccaaggc acccggtgag
     1441 ggtacaggcc ttggtctggg aattatccgc aacgtggtgc taaagcacaa tggcctgctg
     1501 gccttttcaa gccagccagg ttgtacgcgc ttcacgattg ccttgcctgt ggagcacgta
     1561 ccccccggcg aagcggaata aactggcccg cgcggtaggc tggcagaact tttttactcc
     1621 tcggggggag ctaaaacagc tttaatttgg tcgaggccca cgcggttcac aaaatcgccg
     1681 aaacgttcgc ccgcgttact ttctgcagcc cagaaggcga aagctttgtc gagataacca
     1741 ggcagctcct gcatctgcac tttctgcaga accgcttcac ccacgcgcgt accgaggtgg
     1801 ttgccaccga gaaagatcgc gtatttaccg ccgttctgct gcccgacgat gccgatttcg
     1861 gcagagtacg gtcgggcgca accattggga cagccggtca tgcgcacgag tggggctcgg
     1921 tccatcaggc cgtgtttctc aacgacacga ttgatgtcgc gcagcacgtg cggaaagtag
     1981 cgttcgcttt cagtcagggc gagcgcgcag gtcggtaacg atacacaggc gagcgcgcgg
     2041 cggtagagcg gattcgggcg gttggtgttt attccgtgcg cgcgaaaaat atgcaccagt
     2101 tcatggcgtt gcttctcgtc gaggtccagg aacaggaggt cctggtcggg cgttacctgc
     2161 aacgcgcact tggtgcgcgc caccaccagc tggatcgccg tcttcagccg gtagccggga
     2221 atatccttaa tacggccgtt catgatgtag agtccgaagt tataagtgcc atccggcttc
     2281 ctgatgaagc cgtggtgctc gggaatcttc catggcggta acgggcgcgt tgtgttgagt
     2341 tttacctgcg agcgctgttc aatttctgtc ctgaaccatt cgacgccccg gtctgccaga
     2401 acgtacttga ggcgagcgtg cttccggttc gtccggtcac cccagtcacg gtgtgccgtg
     2461 acaatggcgg tcgcgaccgg cagaagtgca tcgaccggta tccagcctaa caaatcagcc
     2521 gcgcgcggga atgtctcagg cttattatgc gtcatgccgc cgccaccgcc ggcaaagacg
     2581 aaaaaaccgt caattttgcc attggtgtgc gtcgccgcga agcccatgtc gtttgcatac
     2641 agatcgacgc cgttttcgcc cgcgaggcag acggcgatct tgaacttgcg cggcaggtag
     2701 gtcttgccgt agatgcgttc ctgttgtacc gggttctgct gcacatcgtc cagccagatt
     2761 tccacgtagg cggttgtctc aaatttgaaa tagtccgaaa gctgctgcga aaagaagctc
     2821 agttgattat agaggggatt accggtaata ttcagtgcct ctgtcacgtt gcgcacaaca
     2881 tccccacaag cccccatact ggtgaggccg acgcggtgga tttcctggat cactgccttc
     2941 aggtcttctt tgagcaggcc gtgcagctgc accgattgcc gcgtcgtcag gcgcagcgcg
     3001 ccgccgccat agcgttcgcc gaggtaatcc catgccaggt actgttcggc ggtcaggcgg
     3061 cctccgggta tacggccgcg cagcatgaag gtattgcgct tttcgggctc accctcggct
     3121 ttcttgtccc ggtccttttg ctggtagaga ccatggaatt tgagcagctg cttctcttct
     3181 tcttcaaact tgtccgtgcc gttgacaaga tcgcgcgcaa tattgccgcg caggaaattc
     3241 gaggcctctt taacgccttc gacttcagag agttcagtgg tctgcttgct catgtttctg
     3301 gcccctttcg ctgcgaaaga acttttgttc aatatcgcgt acgatgtttg ttatgacgga
     3361 ttttttttca ctgacggact gtaaagcgat ggcgcgtttt cttaagtcaa acacgcgcgc
     3421 ccagtcgtcg tccagttcgg gaggcagaac ttcttccagg tgccgcctca ggatggcact
     3481 gaagcccgca aaacgcccct gggtggaaac agacagcgtc accgggccgc ggtcgatcag
     3541 agcattggta aaaaaatcgc accgtgcccg gtcgtcggca ctgttgatca gaatctgttt
     3601 ctgccggcaa agctggaata tctgttcggc cactgcctcg tcatcaacgg cactaaagac
     3661 gataaacttg ccttcgatat cctgtggggt aaagggccgc tcgtggatcg tcaccgaagc
     3721 cagcgtggcc gcctgtagag cctcatgaaa gtcagacttg agctcacggg cgacaattgc
     3781 cagccggcag gcagttcggc gcagctgcac gtatttctcc agcgcggcct tgccgccgcc
     3841 cacgagcaga atttcgcggc cgtccaggtc aaagctgagt ggcagcagca tagtcggctt
     3901 cggtataggc ggtttctgca gccagcgcat cacctaagag cagaatgcct ggcccatgag
     3961 tactgcgcga aattgcccct gcagcagcat ctgcgacaga cccccggctt acgtggcggt
     4021 gctcagaacc cgcattctcc accaggacgc acggtttgct ttcgtcgata ccgttcgaga
     4081 tcagtagttg cgcaatttct accgaccgac ctgcacccat gtaaaagacc agggtgcctg
     4141 ggaaagttgc catactcgcg gcatgtcttg ctacatcttc tgaatggccg tcggtgatcc
     4201 agatgt
//
LOCUS       DAOQJF010000293         4192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12874_length_4192_cov_5.466763, whole genome shotgun sequence.
ACCESSION   DAOQJF010000293 DAOQJF010000000
VERSION     DAOQJF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12874_length_4192_cov_5.466763"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="ilvN"
                     /locus_tag="PLF85_13535"
     CDS             <1..518
                     /gene="ilvN"
                     /locus_tag="PLF85_13535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNE20739.1"
                     /translation="ISLYVPFRRESLPVMKHVLSVKVNNSAGVLSHVTGLFTRRSYNI
                     DSLCVGATNDPRFSVITLVIHEDAAMVEQITKQLLKLIDVIEIQNLTGEISVKRELLL
                     ISLEIKKQERAELLMLSRLFETTVIEMSDTQIVLQMVAEPRRVSHFLEAIRHFKVNHM
                     ARTGVVALALP"
     gene            515..1636
                     /locus_tag="PLF85_13540"
     CDS             515..1636
                     /locus_tag="PLF85_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20740.1"
                     /translation="MTKRPVFRFWSLFLLLLAACGPTPEKFIEKAQVALAKEDFDAAL
                     LYMKNAYELSLPKEFFLTKRDLSFAFLRASHDGQRVLLVEKKAPKKVYENLKVTFIDR
                     AQDKQKRNSIAGKIRDVNFAPNGLHAVFLQQAAANAKECKIWLWQIEKDKVSEVGSSH
                     CVTKPAVKDDGTVWYMRNDQVHIYNPATEKDSLFNKGKKPEKSARKFPAYAYFHAAPD
                     NKVWMIYGAAGSYRLYQLSDKMKLISKEIAAHRLYLVAESRIPGVFIGGAGAQQFVTL
                     NPKDSQIGRRMPAKAWGDAAFVNDKAFYYIEDGVLAYRNGSNETELPFWAEQLAIGIK
                     DELLFLSSTGSALRYFHKKPPLESLKIYNKAVEIDDSKS"
     gene            1633..2991
                     /locus_tag="PLF85_13545"
     CDS             1633..2991
                     /locus_tag="PLF85_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE20741.1"
                     /translation="MIYIVFFLIAGAAALYLLYSDSRKSRNETLNADPHQVIRDRQAA
                     IRLSLNDLQYELSVGKVEPQDFARLQSELLAEWEALEGRLASLPAPQKKPADECTKCG
                     ATLLANARFCHACGAKVLQLLLVLLLWPAGQLEALDIRVQVKNGTTGKVHTQPLKIQL
                     IKLEKGMQPVAANTSSNGQTEFLQLPENPMGPYMVQTEYRSVTYSKVIPPNMPSPAEV
                     TLDIYESTASTDRLRVRTLVEVRRVAEDKLAGIMILFFMNQDNRTFTGNGGLEFFLPA
                     QAVVEQASVSVGSGASNIQWLKVAAEKTGAAGAYRIAQSVKPGERIVQVMFSLPYRPA
                     GTSLAFRSVYPQDAGLQLIAEPENLEVRQADRLLARVKDPNLGRGLISFPSAESRIEL
                     KFSGGSIAEARAEESEAELVLRSPLEVWQKLLFPLLALITFFTVFYLREKLRTDKSGD
                     AD"
     gene            complement(2988..3725)
                     /locus_tag="PLF85_13550"
     CDS             complement(2988..3725)
                     /locus_tag="PLF85_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20742.1"
                     /translation="MADLVSRRMMKNLLTRRAGGARVVTENVAVLKAIETVPKIASVE
                     SDNYYKEPVTIEYYIPKESRFAYQVKYLYVPLYDPTPRNDNARLVMNHFKALGEPIDL
                     MKVMDEFPQFLVKMLDYLSPQMGIIENLSRSIQDGLGGEQDAFRRALYTCEVLRKFEP
                     SIVSLEIAGDYTTYNINWLVRKLNSLQIEFSLEDPTVEFLIQRYRQFANETGEVIPER
                     FEILSQIYLDQAFPMHDEDFADLMGQD"
     gene            complement(3764..>4192)
                     /locus_tag="PLF85_13555"
     CDS             complement(3764..>4192)
                     /locus_tag="PLF85_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20743.1"
                     /translation="QPYATFSLKERLLTGRPFATAILRLASQSAGIDARSARTAIRVL
                     REHFGAETAPVIARADESTLVIIFAPETGNAGAAATALRQLFSAYRSQAKNPVAAIVE
                     DSPSCGSAQRVLKTLEMLLAQSEKSGEVAALRQQERLHIL"
BASE COUNT          960 a         1108 c         1165 g          959 t
ORIGIN      
        1 ccatttctct ttacgttccg tttcgccgtg agtccctgcc tgtaatgaag cacgtgcttt
       61 ccgtaaaggt taataactcg gccggtgtgc tgagccatgt gacgggcctt tttacccgtc
      121 gcagctacaa tatcgattcg ctttgtgtcg gcgccaccaa cgacccgcgc ttttctgtga
      181 tcaccctggt gattcacgaa gacgctgcta tggtcgaaca gatcaccaag cagctgctca
      241 agctgatcga cgtgattgag atacagaacc tgacgggtga gatttccgtt aaacgtgaac
      301 tcctgctgat ttcactcgaa atcaagaaac aagagcgcgc cgagcttttg atgctttcgc
      361 gcctgtttga aaccaccgtg attgagatgt ctgacactca gatcgtgctg cagatggttg
      421 ctgaaccgcg gcgtgtcagc catttcctcg aggcaatccg ccattttaaa gtgaaccaca
      481 tggcgcgtac gggtgtcgtg gcgcttgccc tgccatgacg aagcgcccgg tcttccgttt
      541 ctggtcatta ttccttctgc tgttggctgc gtgcgggcca acacccgaga agtttattga
      601 aaaggcgcag gttgcgctgg cgaaagaaga tttcgatgcg gcattactct acatgaagaa
      661 tgcatacgaa ctctcgctac ccaaagaatt ttttcttacc aagcgcgatc tttcctttgc
      721 tttcctgcgc gcctcacacg atgggcagcg cgtcttgctg gtcgagaaaa aggctcctaa
      781 aaaagtgtat gaaaacctga aggttacttt catcgaccgc gcacaagata aacagaaacg
      841 caacagtatc gccggcaaga tacgtgacgt caattttgcg cccaacggcc tgcatgctgt
      901 gtttttgcag caggccgcgg cgaatgcaaa agaatgcaag atctggctat ggcagattga
      961 aaaagacaag gtgtcagaag tggggagctc tcactgcgtg accaagcctg cagtcaagga
     1021 cgacggcacc gtttggtaca tgcgcaacga ccaggtgcat atttataatc ccgcgacgga
     1081 aaaagactcg ctgttcaata aggggaaaaa accggaaaag tctgcgcgga aatttccagc
     1141 ctacgcctat tttcatgctg ccccggacaa caaggtttgg atgatctacg gtgctgcagg
     1201 cagctaccgt ctctaccaac tgagcgataa gatgaagctc atttcgaaag agattgccgc
     1261 gcatcgcctg tatctcgtgg cagagagtcg cataccggga gtttttatcg gcggggcagg
     1321 ggcgcagcag tttgtcacgc ttaacccgaa agattcacag atcggcaggc gtatgccggc
     1381 aaaggcctgg ggcgatgcag cgttcgtcaa tgacaaggca ttttactaca tcgaggatgg
     1441 cgtccttgct taccgcaatg gcagcaatga aacggagttg ccgttttggg ctgagcaact
     1501 tgcgattggt atcaaggacg agctgctgtt tttgtcctcg acgggttctg cgctgcgtta
     1561 ttttcacaag aaaccaccat tggaatcgct caaaatctat aacaaggcag tcgaaatcga
     1621 cgacagtaaa tcatgatcta cattgttttt tttctcatcg cgggggctgc cgccctctac
     1681 ctgctttatt cggatagcag aaagtcgcgc aacgaaacat taaatgcaga cccacaccag
     1741 gtaatacgcg accggcaggc agcgatccgc ctgtcgctga atgacctgca gtacgagctg
     1801 tcagtgggta aggttgaacc tcaggatttt gcgcgcctgc agtctgaatt actcgccgaa
     1861 tgggaggcgc ttgaaggcag gttggcctct ctgccagccc cgcagaaaaa gcctgcagat
     1921 gagtgcacca aatgcggcgc cacgctgttg gccaacgcca ggttctgcca cgcttgcggg
     1981 gcgaaggtcc tgcagctcct gttggtactg ctgctctggc ccgcaggtca gctcgaagcg
     2041 ctcgatatac gggtgcaggt caagaacggc acaaccggca aagtgcatac ccagccgctt
     2101 aagatacagc tgatcaagct cgagaaaggt atgcagccgg tggcggccaa taccagcagc
     2161 aatggccaga cggagtttct gcaattaccg gaaaacccca tggggcccta catggtgcag
     2221 accgaatacc ggtctgtaac ctacagcaag gttatacctc ccaatatgcc ctcgccagcc
     2281 gaagtcacgc tcgatatata tgaaagcact gcctcgaccg atcgcctgcg cgtgcgcacg
     2341 ctggtcgagg tgcgccgcgt agcagaggat aagctcgccg gcatcatgat cctgttcttt
     2401 atgaaccagg ataatcgcac gttcaccggt aatggtggcc tggaattttt cctgccagcg
     2461 caggcagttg tagaacaggc ttcagtttcg gttggcagcg gcgcctcgaa catccagtgg
     2521 ctgaaggtcg cagcagagaa aacgggcgcg gccggtgcct accgaattgc ccagagcgtt
     2581 aagccgggtg agcgcattgt gcaggtgatg ttcagtttgc catacaggcc agcgggtact
     2641 tcgctggcct ttcggtctgt ttatcctcag gatgccggac tgcagctgat tgccgagccg
     2701 gaaaatcttg aggtgcgcca ggccgaccgg ctgctcgcgc gcgtgaaaga tcccaacctc
     2761 ggccgcgggc tcatctcgtt cccatcggct gaaagtcgca tcgagctaaa atttagcggc
     2821 ggcagtattg cggaagcgcg cgccgaagaa agcgaagccg aactggtttt gcgctcaccg
     2881 ctcgaagtct ggcagaaact actgttcccc ctgctggcgc ttatcacctt ctttacggta
     2941 ttttacctgc gcgagaaact gcgcacagac aagagcggcg acgcagatta gtcctgaccc
     3001 attaggtcgg cgaaatcttc gtcgtgcatt gggaaagcct ggtcgaggta tatctgcgag
     3061 agtatctcaa aacgctccgg aataacctcg cctgtttcat tggcgaactg ccggtaacgc
     3121 tgtatcagga attcgacggt cgggtcttca agagaaaact cgatctgcag ggagttcagt
     3181 ttcctcacca gccagttgat attgtaggta gtatagtcgc cagcgatttc gagcgaaaca
     3241 atcgatggtt caaatttgcg tagtacttcg caggtataaa gcgcgcgcct gaaagcatcc
     3301 tgctccccgc caagaccatc ctggatgctg cggctgagat tttcgatgat acccatctgc
     3361 ggtgaaaggt agtcgagcat cttcaccaaa aactgcggaa attcgtccat gaccttcatc
     3421 aggtcgatgg gttcgcccag tgccttgaaa tgattcatga ccagccgcgc gttgtcgttg
     3481 cgtggtgtgg gatcatagag cggcacgtaa aggtatttta cctggtaggc aaaacggctt
     3541 tcttttggta tgtaatattc aattgtgacc ggctctttat agtagttgtc cgattcgaca
     3601 ctggcgatct tggggacagt ctcgatcgct ttgagcacgg cgacgttttc tgtcaccacg
     3661 cgtgcgcccc cggcgcgccg cgtgagcagg tttttcatca tccgccgtga gacgagatct
     3721 gccataattt aagtataagc ctgttgcgtt tgtgcggcca ttttcagaga atatgcaggc
     3781 gctcttgctg tcgcagcgca gcaacttcac cgctcttctc tgactgagcg agcagcattt
     3841 cgagcgtctt gagcacacgc tgtgcagagc cgcacgaagg cgaatcttct acaatggcag
     3901 ctacggggtt ctttgcctgc gagcggtagg ctgaaaacag ctgccggagt gcagtcgccg
     3961 cggcacctgc attgcctgtt tcgggtgcga agataatgac gagggtagat tcgtcggccc
     4021 gggcaataac gggtgccgtt tcagcgccga agtgttcacg caggacacgg atagctgtgc
     4081 gcgccgaacg tgcgtcaatg ccagccgact gtgatgccag tcgcaggatt gcagtggcaa
     4141 aaggccggcc ggtcagcagg cgctccttca gcgagaatgt agcataaggc tg
//
LOCUS       DAOQJF010000294         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12886_length_4190_cov_4.524547, whole genome shotgun sequence.
ACCESSION   DAOQJF010000294 DAOQJF010000000
VERSION     DAOQJF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12886_length_4190_cov_4.524547"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLF85_13560"
     CDS             complement(<1..780)
                     /locus_tag="PLF85_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNE20744.1"
                     /translation="MIYPIDTDIEDMAEETRAIEGKKRALDRCRPLSSAVVKRLEADF
                     NLEMTFNSNAIEGNTLTLHETDMVLRRGITIGNKTLREHFEARNHGKALVFLRSVIAK
                     RQKFSKELILQLHHEIMREIDTDAGLFRRTQVRILGTNHIPPNHLKVEKLIDQLVEWY
                     YANENRVPAWLLAATVHFKIAHIHPFADGNGRTARLLMNLVLLQKGYPPAVILHVDRK
                     KYYRVLREADAGRPKNFYAFVARAVDRSLSIWLSALSTGKKS"
     gene            929..1414
                     /locus_tag="PLF85_13565"
     CDS             929..1414
                     /locus_tag="PLF85_13565"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNE20745.1"
                     /translation="MTPQKRNTAIYIGKITTAILLCYVLSHAVSRIDYIWALISAILV
                     MSPEGKDAVELSVIRIKGNIVGVLVGVLFLLVALENPVNIIAGAAVALFVCHRLKLMP
                     GARATLVALVITLMQTDPTDHWGSAATRVAAVVAGCAIALVATELFHNVLKLRYTEPT
                     N"
     gene            1416..2273
                     /locus_tag="PLF85_13570"
     CDS             1416..2273
                     /locus_tag="PLF85_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20746.1"
                     /translation="MSEPEFRDREEAWEYYAQQQKKAWESLSEEELIAQVERGNYDLF
                     FTLPDVVAAKLPREKAIFTLFKKVLRQETDYLVNYHTLDQLIKLLGEPKGIEDAAFDD
                     LYTRVNRGFYGEEKRLAACRELWQKIVAQYPKLAAEEPDFRVPESFLNHRFEIVYRHG
                     GSNFSFYHKDKPFSVATFEEVLRAKFEKFRTWAYTADDAQLTFHEDDTNRWAVMTGFN
                     FNCKVTIGHASKQRRLEQDQSSGPWYLNNPIVLVKKGERNYDLESTLADAITNPLGIS
                     ELDYSRFNT"
     gene            complement(2283..>2719)
                     /locus_tag="PLF85_13575"
     CDS             complement(2283..>2719)
                     /locus_tag="PLF85_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20747.1"
                     /translation="STVLHHYANKQKLYEQVVAESIDFFSRMPGAERQSVSLAERLKY
                     LLGQMVAEPMHAKLLNRVFMDNPKSAGMAARRYWRPLLKHLAGSDDPTTQATAMFCVN
                     AIIQIAFSIELQREILGRCSAEEIAQRYSQVMDQLIAGMLTR"
     assembly_gap    2720..2819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2983..3996)
                     /locus_tag="PLF85_13580"
     CDS             complement(2983..3996)
                     /locus_tag="PLF85_13580"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HNE20748.1"
                     /translation="MELKPKDILSSEEMAQLLKKSDLRGFAEVGTTWALVAFSFVIVA
                     LWPHTPTLFVPVLIALVLLGNRHLALAILVHDASHHALFKTRWLNDFMGKWFAAAPVL
                     QNLEGYRKYHLQHHRYTGQDYQGEKGDPDIILTRNYPISKKSLRRWLLRDLTGRSAPR
                     LYLGLLAMNLGLIRFDLSGRVVMERRERRSVLGWLKHGTLSLFPFLLTNAAMLTALIL
                     LGHGWLYLLWLAAGFTTFQFFVRVRSIAEHGMLPRVADMLRNTRTTEVAWWERLTFAP
                     HHVNYHLEHHFLMAVPSYRLPQMHKILKERGVFKDAPLARGYAEILRLATQKPDQDAG
                     ATA"
BASE COUNT          893 a         1144 c         1139 g          914 t
ORIGIN      
        1 tgattttttt cccgtcgaca gggcgctcag ccagatcgag agtgagcgat cgactgcgcg
       61 tgcgacaaaa gcgtagaagt tctttgggcg ccccgcgtcg gcttcgcgca gcacgcggta
      121 gtacttcttg cggtcgacat gcagaatcac cgccggtgga tacccctttt gcagcagcac
      181 caggttcatc agcaggcgcg ccgtgcgtcc attgccgtcg gcaaacgggt gaatgtgcgc
      241 aatcttaaag tgcacggttg ccgcaagtag ccacgccggt acccggttct cgttggcgta
      301 gtaccattcg acgagctggt cgatcagctt ttcaaccttc aggtggttag gcggaatatg
      361 gtttgtgcct aatatgcgta cctgcgtgcg cctgaaaagg cccgcatcag tatcaatttc
      421 gcgcataatt tcgtggtgca gctgtagaat cagttctttg ctgaacttct gccgtttcgc
      481 aatgacactg cgcagaaata ccagcgcctt tccatggttg cgtgcctcaa aatgttcgcg
      541 caacgtcttg ttgccgatcg tgataccccg ccgcagcacc atgtcggttt cgtgcagcgt
      601 cagggtattg ccctcgatcg cgttgctgtt gaaggtcatc tcgaggttaa aatcggcctc
      661 aagcctcttg actactgccg acgacagggg gcggcagcga tcgagcgcgc gttttttgcc
      721 ctcgatggca cgggtttctt cggccatgtc ttcaatatcc gtatctatcg gatagatcat
      781 aatcaagtat atccgatata acgtaagccg gcaagtaaaa tatatcggat acaaaaaatt
      841 acacttatcc gatatattgt ccaaagcccc tttctgcttt tcttggcagc ctgcgggttt
      901 tctgcaacat agccgtgaaa ataagcctgt gaccccgcaa aaacgcaata ccgccatcta
      961 catcggcaaa attaccaccg ccattctgct ctgttatgtg ctctcgcacg ccgtcagtcg
     1021 catcgactac atctgggcgc tgatttcagc gatactggtg atgtcgcccg agggtaagga
     1081 cgctgtcgaa ctttctgtca ttcgtatcaa gggcaatatt gtcggtgtcc tggtgggagt
     1141 tctctttctt ctcgtcgcgc tcgaaaaccc ggtgaacatc atcgccggcg ccgccgtggc
     1201 ccttttcgtc tgccaccggt tgaagctgat gcccggcgcg cgcgcgaccc tggtcgcact
     1261 ggtgataaca ctgatgcaga cagaccccac cgaccactgg ggttcggcgg caacccgcgt
     1321 cgctgcagtc gtcgccggct gcgcaattgc ccttgtcgcc acagagctct tccacaacgt
     1381 attgaaatta aggtataccg aacccactaa ttaaaatgag cgagccagaa ttccgcgatc
     1441 gagaagaagc ctgggagtac tatgcgcagc agcagaaaaa agcctgggaa agcctgagcg
     1501 aagaagagtt gattgcacag gtcgagcgcg gcaattacga cctgttcttc acactgcccg
     1561 atgtcgtggc ggcaaaacta ccccgcgaaa aggcgatatt caccctgttc aaaaaggtgc
     1621 tgcggcaaga gaccgactat ctcgtgaact accacacgct cgaccagctc atcaagctgc
     1681 tcggtgagcc gaaaggaatc gaagatgcag cctttgacga tctctatacg cgggtgaacc
     1741 gtggtttcta cggtgaagag aagcggctcg cggcctgccg cgaactctgg cagaagattg
     1801 ttgctcaata cccgaagctc gcagcagagg agcccgattt tcgcgtgccc gaaagttttc
     1861 tgaaccaccg cttcgagatt gtctaccggc atggcggcag caacttctct ttctaccata
     1921 aagataaacc gttcagcgtc gcgacattcg aagaagtcct gcgtgcgaag ttcgaaaagt
     1981 tccgtacctg ggcatacact gcagacgacg cgcagctgac ctttcacgaa gacgacacaa
     2041 accgctgggc agtgatgacg gggtttaact ttaactgcaa ggtcaccatc ggccacgcgt
     2101 caaagcagcg ccggcttgag caagaccagt catcggggcc ctggtacctc aataacccga
     2161 tcgtgctggt caaaaagggc gagcgcaact acgacctcga aagcacgctc gccgatgcta
     2221 tcacaaatcc gctgggaatc agcgaactcg attattcacg gttcaatacg tgactatgcc
     2281 cgtcagcgag ttagcatacc ggctataagc tggtccatta cctggctata gcgctgcgct
     2341 atttcttctg cgctgcagcg accgagaatt tcccgctgca gttcgatact gaaagcaatc
     2401 tgtatgatgg cgttgacgca gaacatggcg gttgcctgcg ttgtggggtc gtcactgccc
     2461 gccaggtgtt ttagcagcgg acgccagtag cgccgtgcgg ccatgccggc actcttgggg
     2521 ttgtccatga agacgcggtt cagcaatttg gcgtgcatgg gttcggcgac catttgtccg
     2581 agcaggtatt ttaaccgttc ggccaggctg accgactgcc tttcagcgcc cggcatccgg
     2641 gagaaaaaat cgatactctc tgcaacgacc tgctcgtaga gtttttgttt gttcgcgtag
     2701 tggtgcagta ccgtcgactn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2821 cgacctgctc gtagagtttt tgtttgttcg cgtagtggtg cagtaccgtc gacttggcga
     2881 ttctcgcgcg ggtcgcaatg tcagcaatgc tcacaccggc gtagccgcgc gctgcgaaca
     2941 gctcctgtga aacgaggtaa atccgcctat gtatgtcagt catcaggcag ttgcccctgc
     3001 gtcctggtca ggcttctgtg ttgcgaggcg tagaatttct gcataaccgc gcgccagcgg
     3061 agcgtctttg aatacgccgc gctctttcag tatcttgtgc atctgcggca ggcggtacga
     3121 gggtactgcc atcagaaaat ggtgctcaag gtgatagttc acgtggtgtg gcgcgaacgt
     3181 cagacgctcc caccacgcaa cttctgtggt gcgcgtattc ctgagcatat cggcgacacg
     3241 cggcagcatg ccgtgttcgg caatggagcg aacgcgcacg aaaaactgga aggtcgtgaa
     3301 gccggcagcg agccacagca ggtagagcca gccatgcccc agcagtatca aggcggtcag
     3361 catcgccgca ttggtcagca agaaagggaa gaggctcaat gtgccgtgct ttaaccaacc
     3421 gagcacgctg cgccgctctc gccgttccat cacgacgcgg cccgaaaggt caaaccggat
     3481 gaggccgagg ttcatcgcca gtagcccgag gtaaagccga ggtgccgagc ggccggtcag
     3541 atcgcgcagc agccacctgc gcaggctttt tttcgaaatg gggtagttgc gcgtgagaat
     3601 aatatcggga tcaccttttt caccctggta gtcctgcccg gtgtaacgat ggtgctgcag
     3661 gtggtacttg cggtaacctt cgaggttctg cagtaccggc gcagcggcga accacttgcc
     3721 catgaaatca ttgagccagc gtgttttgaa cagcgcatgg tggctggcat cgtgcacgag
     3781 aatcgcgagc gcgaggtgcc ggttgccgag cagcactagt gcaatgagta caggtacgaa
     3841 aagcgtcggt gtatgcggcc acagcgccac gatcacaaac gaaaaggcga ccaaggccca
     3901 ggtggtgccg acttcggcaa aaccgcgcag gtcagatttt ttcagtagct gcgccatttc
     3961 ttcgctgctc agaatgtcct ttggtttaag ttccacaata cctccgctgt gccaatatcg
     4021 accgaacgat cggtcagtat cgtggtgtca agcgtttgcc gggcgaaatt ctgcggccgg
     4081 ggcgggcagg gcgaaatcac cccggctgcc cgtcgccttg agtcagacca ctttactgtc
     4141 gataatatac ccacaggtcc gtcctatatg ctgatcatgc gccccctatg
//
LOCUS       DAOQJF010000295         4185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_12917_length_4185_cov_4.069249, whole genome shotgun sequence.
ACCESSION   DAOQJF010000295 DAOQJF010000000
VERSION     DAOQJF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12917_length_4185_cov_4.069249"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1782
                     /locus_tag="PLF85_13585"
     CDS             82..1782
                     /locus_tag="PLF85_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE20749.1"
                     /translation="MIRSNYFTDNADLQQHIEELIDWNDVISTYENGFQDAAEYQKTK
                     NPRLEMAPSSNEEALAYYKEILQSAGEIAGLHTSQAAQAIDREGLKFDNGVVTHPQKM
                     MEVIQKYREAGLPPVAFRRKYGGLGLPGPVKAMASELMYRADTSVTIAMGSMGLAGIL
                     EKYASEEAKDRWIPKIIDENYCVAMGLTEPDFGSDLPSIRTKAVQRDGQWYLTGTKRF
                     QTVACGLNGGPALMLALARTTEGSSGAKGLSFFIVESKHYKITGIEKKLGLKASATCE
                     VALEDAPGELVGQEGYGLVRYVIGMLNGARMGIAAQGVGLATAAYMEAKKYAAERIQF
                     GKPIAQIPAVGRMLERMEREIAAMRCLMIEGASIVDRYQCPIANGVKESEATRAAEKL
                     ANVITPISKYYNSEMCNDLVDDALQVFGGSGYTEDYDLARLYRDARITNIYDGTTQIQ
                     VNAAIGGVVAGMAPTGVWRSYLDTLTSTVGKNDAIGEIRAKFEDVVSTWRALEDRERK
                     EALSFEVVESAARLVNSLLMEKAALRASNKTERARLNTAYHVDSLATVAGNLIRLKAG
                     "
     assembly_gap    1851..1950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2042..2374)
                     /locus_tag="PLF85_13590"
     CDS             complement(2042..2374)
                     /locus_tag="PLF85_13590"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE20750.1"
                     /translation="MKKFKVVWAAGARADFELLIQYLIDRESAVAEKIFEALLAQADS
                     LETAPERGRKVPELLSLGVVDVREIFYKPWRIIYRIRDNEVRILMVIDGRRSLEGELL
                     RNLTGRPL"
     gene            complement(2371..2658)
                     /locus_tag="PLF85_13595"
     CDS             complement(2371..2658)
                     /locus_tag="PLF85_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNE20751.1"
                     /translation="MNLAEDIKPISYVKAHAAEIIEKMAENHRPIIITQNGEAKAVLT
                     DIETYQRQQETMAMLKMVALRERDIEESGGIPHEEAKKHFREYFRELKSKR"
     gene            complement(2726..3154)
                     /gene="tnpA"
                     /locus_tag="PLF85_13600"
     CDS             complement(2726..3154)
                     /gene="tnpA"
                     /locus_tag="PLF85_13600"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNE20752.1"
                     /translation="MSRHAYQVLIYHVVFATKRRAPLLTEANMQQVKDSLATKANDLG
                     IDILALNGYVDHMHILLKLPAAKSLTSIVGQLKGYSSRRNANLRWQIGYAAFTVSASA
                     VAAVRQYIQEQQRHHESMDYRGEMSALKKRAAPLAMGLTP"
     gene            complement(3674..>4185)
                     /locus_tag="PLF85_13605"
     CDS             complement(3674..>4185)
                     /locus_tag="PLF85_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20753.1"
                     /translation="GNCVRCHSGPNFSDGSFHNLGVPQIGGALGVMDDGRYTGISDLL
                     TGSNSTFNTNSGFNATPSVNRVSGLARTDADIGKFKTPTLRSVNKTPPYFHNGTFNSL
                     WDVVNFYNFAGNAGNFPGTKDPILTTRRMTNEEMEDLVNFLKALEGEALPTSLTTSPG
                     GFGTTTNWP"
BASE COUNT          934 a         1135 c         1095 g          921 t
ORIGIN      
        1 tagtgcgttc aagggaaccc ctgttcatgg gggttttgcg cgaatgaatt cccatcgcat
       61 cgccgatttg tgccgttgcc catgatccgc agcaactact tcaccgacaa cgccgatctg
      121 cagcagcaca tcgaagaact cattgattgg aacgatgtta tcagcacgta cgagaatggc
      181 ttccaggacg ccgcagagta ccagaagacc aaaaatccgc ggctcgagat ggcgccctca
      241 tcgaacgaag aggcgttagc ctattacaag gagattctcc agtcagctgg ggagatcgcc
      301 ggcctgcaca cctcgcaggc ggcgcaggca atcgaccgcg agggcctcaa atttgacaat
      361 ggagtagtta ctcacccgca aaagatgatg gaggtgatcc agaagtaccg cgaagcaggc
      421 ttaccccccg tggcatttcg ccgcaagtac ggtggcctgg gtctgcccgg cccggtgaaa
      481 gcgatggcgt cggagctcat gtaccgcgcc gacacgtcgg taacgatcgc gatgggcagc
      541 atgggcctcg cgggcatctt ggaaaagtat gcatctgaag aagccaagga ccgctggatt
      601 cccaagatca tcgacgaaaa ctactgcgtc gcgatggggc tcaccgaacc cgactttggt
      661 tctgacctgc cctcgattcg caccaaggcc gtgcagcgcg acggccagtg gtatttaaca
      721 ggtactaaac gcttccagac cgtcgcgtgc ggcctcaacg gcggccccgc gctgatgctg
      781 gcgctcgcgc gcaccaccga aggttcgtcg ggtgccaagg gcctgtcgtt cttcatcgtc
      841 gaaagcaagc actacaagat caccggcatc gagaagaagc tcggtctcaa ggcctcggcc
      901 acctgcgaag tggcgctcga agacgccccg ggtgaactcg tcgggcaaga aggttacggc
      961 cttgtgcgct atgtcatcgg catgctcaac ggcgcgcgca tgggcatcgc ggcgcagggt
     1021 gtcggtctgg caaccgctgc gtacatggaa gcaaagaagt acgcggcgga gcgcatacag
     1081 ttcggtaaac cgatcgcgca gataccggcc gtgggccgaa tgctcgaacg catggagcgc
     1141 gagatcgcgg ccatgcgctg cctcatgatc gaaggtgcct cgatcgtcga ccgctaccag
     1201 tgcccgattg cgaacggcgt caaagaaagc gaagcgaccc gcgcggccga aaagctcgcg
     1261 aacgtgatca ctccgatttc aaagtactac aactcagaga tgtgcaacga cctcgtcgac
     1321 gacgcgctac aggttttcgg cggctcgggt tataccgaag actatgacct cgcgcgcctc
     1381 taccgcgacg cacgcatcac caacatctac gacggcacga cacagataca ggtgaacgcg
     1441 gcgattggcg gcgtggttgc gggcatggca cccaccggcg tctggcggag ctacctcgac
     1501 accctgacct ctaccgtggg caaaaatgac gcgataggcg aaatcagggc aaaatttgaa
     1561 gacgttgttt cgacctggcg tgccctcgaa gaccgcgagc gcaaagaggc actctctttc
     1621 gaagtcgtcg aatcagccgc gcgcctggtc aattcactgc tgatggaaaa ggcagccctg
     1681 cgcgcgagca acaagaccga acgcgcccgc ctcaataccg cctaccacgt cgactcactc
     1741 gcgacggtgg cgggcaattt gattcggctg aaggcggggt gattcctcta gctacgggct
     1801 tacctgtggt gcgacgagcg aagcgagtct ctgctttcag agcttgtcga nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggcttacct gtggtgcgac gagcgaagcg
     1981 agtctctgct ttcagagctt gtcgaaccaa gcccatagct agtattcccc agcgttcttg
     2041 ttcagagagg cctgccggtc aggttgcgca gtaattcccc ttctagactt cgtctgccat
     2101 caattaccat cagtattctg acctcattgt cgcgaatgcg atagatgata cgccagggct
     2161 tatagaaaat ctcccgcaca tcgaccacac cgagcgatag cagttctgga acttttcgcc
     2221 ctcgttcagg tgctgtttcc agagaatccg cttgagccag tagagcctca aaaattttct
     2281 ctgcaacagc agattccctg tcgattagat actgaattaa aagttcaaaa tctgcccggg
     2341 ctccggcggc ccagactact ttgaatttct tcatctcttt gacttgagtt cacgaaaata
     2401 ttcccgaaaa tgcttttttg cctcttcatg gggaatgcca ccgctttctt caatatcgcg
     2461 ttcacgcagc gccaccatct tcagcatagc catagtttcc tgctgtctct ggtacgtttc
     2521 aatatcggtc agcacagcct tcgcttcgcc gttctgcgtg atgatgatcg gcctgtggtt
     2581 ttccgccatc ttctcaatga tctccgccgc gtgggctttg acatacgaaa tcggtttgat
     2641 gtcttcggcg aggttcatgc cataagactg aattcggttt ttattctgtc aacctgattc
     2701 tgggtcgggg taatgcaccc tttagctatg gggttaaccc catagctaaa ggagcagcac
     2761 gtttctttag cgcactcatc tctccgcgat agtccattga ctcgtggtgc cgttgctgtt
     2821 cctgtatata ttgcctgacg gcagcaacag cgctggcgct gaccgtaaag gcagcatagc
     2881 cgatctgcca gcgaaggttt gcattcctgc gcgaagaata gcctttcagt tggccaacga
     2941 tactggtgag gctcttcgct gcgggcaact tcagcagaat gtgcatgtgg tccacatacc
     3001 cattcagtgc cagtatatca atacccaggt cattggcctt cgttgccaga gaatccttca
     3061 cctgctgcat attcgcctct gtaagcaaag gagcccgccg cttggtcgca aataccacat
     3121 ggtagatcag aacctggtac gcatgtcgac tcacgccaaa caaagttgcc accaacctat
     3181 ccggcggaca agtctttctt tcggctgtgg ctttcttgta gctatgggct taagcccata
     3241 gctacaagaa gacccaccca acgatgcccg tgatgcagat tatcgaaacg aaaaattttt
     3301 cacctatggt tgtctattgg cgaggggctc ctctagctat gggcttaagc ccatagctag
     3361 aggaggcatc accctgacac gcccgcgagg gggatattca atgagaaaat tcttttagaa
     3421 ttttttagct atggggttaa ccccatagct agaagacgca tcatccaagc gcacccccga
     3481 ggcagattat ttacacggaa aatctttccc ctatgggctt ctattggcga tggggttcta
     3541 gtagctatgg gggctctact agctatgggc ttaagcccat agctagtaga ggaacacccc
     3601 cgcgagggag ataatcgatg agaaaattct tttagaatct tttaactatg gggttaaccc
     3661 catagctaaa aggtcatggc cagtttgtcg ttgtgccgaa accgccaggt gaagtagtaa
     3721 ggcttgtcgg aagagcctca ccctcaaggg ccttcaaaaa gttcaccaga tcttccattt
     3781 cttcattggt catgcgacgc gtggtgagaa tggggtcttt ggtgcctgga aagttgccgg
     3841 cattgccggc aaagttgtag aagttcacaa catcccacag gctgttgaac gtgccattat
     3901 gaaagtatgg tggcgtctta ttgaccgacc gcagcgtcgg tgttttgaat ttgccgatgt
     3961 ctgcatcggt tctggcaagg ccggacactc tgttaaccga gggtgtcgca ttaaagccag
     4021 agttcgtatt gaaagtactg tttgagccag tcagaagatc gctgatacct gtatagcggc
     4081 catcgtccat gacgcccaaa gccccaccta tctgcggcac gccgaggtta tggaatgatc
     4141 catcgctaaa gttcggtccg ctatggcaac gcacgcagtt tccct
//
LOCUS       DAOQJF010000296         4119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13221_length_4119_cov_4.762549, whole genome shotgun sequence.
ACCESSION   DAOQJF010000296 DAOQJF010000000
VERSION     DAOQJF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13221_length_4119_cov_4.762549"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="PLF85_13610"
     CDS             complement(<1..100)
                     /locus_tag="PLF85_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNE20754.1"
                     /translation="MAEKRDYYEVLGVPKSASLNEIKSAYRKLAMQF"
     gene            complement(153..2057)
                     /gene="dnaK"
                     /locus_tag="PLF85_13615"
     CDS             complement(153..2057)
                     /gene="dnaK"
                     /locus_tag="PLF85_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNE20755.1"
                     /translation="MAKEKIIGIDLGTTNSCVAVMEGGEAVVIANSEGTRTTPSIVAI
                     TEKGETLVGQVAKNQMVTNPVNTLRSVKRFVGRRFSEVTSEIGKVAYKVKEGTGKSVL
                     IETREKDMRPEEVSARVLMKMKQTAEEYLGTKVEKAVVTVPAYFNDEQRQATKDAGRI
                     AGLDVVRIINEPTAAALAYGLDRDKSGEVIAVYDLGGGTFDISILELADNVFEVKATN
                     GDTHLGGDDFDQAIIDWIVAEFKKETAIDVGKDKMALQRLQEAAEKAKIELSSKETSD
                     INIPFITMDASGPRHLNMTLTRAKFNQLTASLVERSVEPCRKAIADSGYSLNEINKVI
                     LVGGSTRIPAVREKVKELFGKEPDKSVNPDEAVAIGAAIQGGVLAGEVTDVLLLDVTP
                     LSLGIETLGGVMTKIITRNTTIPTKKSEIFSTAADNQTAVDIHVLQGERELAKDNRTL
                     GRFQLSEIPPAPRGNPQIEVTFDIDANGIVHVSAKDLKTNKEQKIKIEASSSLSEADI
                     QKMVKDAEANAEEDRKAREAATAYNDLDHIIYTTEKMVADAPALDAGIKSEVEGAVAK
                     AKEALMSRDADRMKAAAQTLGEVVQKHQAAFSVQGNAPGDDAGTQEKSTGGDEKVVDA
                     EFTEIKDDKK"
     gene            complement(2103..2699)
                     /locus_tag="PLF85_13620"
     CDS             complement(2103..2699)
                     /locus_tag="PLF85_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNE20756.1"
                     /translation="MSESTEQTNNDNAKHFASEAEEIASMEAAAAGSPAGTMDATAEL
                     RQQLESITADLQRERAEFSNFRKRAVQEKAQQAAQSSARLLTDLLPALDAFDQFFAAY
                     LAKAEADAGIKAIVDGVQLVHKQITRVFTESGVEELNPVGAEFDPNLMEALSLVEADV
                     EKETVSQVFQKGYRIEGRVIRPARVVVTKPRATESKES"
     gene            complement(2696..3844)
                     /gene="hrcA"
                     /locus_tag="PLF85_13625"
     CDS             complement(2696..3844)
                     /gene="hrcA"
                     /locus_tag="PLF85_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNE20757.1"
                     /translation="MTDRQGEILRALVKEYVASGEPVGSQVLVEKFSMAFSPATVRKE
                     MSVLEQMGLLSSPHTSAGRIPTDRAFQLYLADLVNLFEITLNQKAELEDLYRSAALQL
                     DQLLKTTAHMLAQTSNFAGIVLAPNSVGTSIKRIELVSVMESLVLMIMISSSGAIYEK
                     KIKLDRSVNQEELYKISRYLNQNLKGHEIGDVQERGLEFLRASLADLGELSDIGMAVT
                     QAIVYNPPDQTIHIEGDANLHRKIYDLTPDRRKAERIISQLANKDYIASVFERMKNQP
                     QVGSQVGLEIDGEILSGITILGKGYTLSGKSIGALGVIGANRMPYEKIIPALDYSSQI
                     LSNVLSEKNNIELQGDELRVNEIEITARKELPEAVAKAKAQPKPKRKQ"
BASE COUNT          846 a         1113 c         1013 g         1147 t
ORIGIN      
        1 ggaactgcat cgccagcttg cggtaagcgc tcttgatctc attcagtgaa gcagacttgg
       61 ggacgcccag gacttcgtag taatcgcgtt tttcggccat cttttaattg tgtgccgtcc
      121 ctcgcggctc agccgcaagg gatggcagta tcctattttt tgtcgtcttt tatttcagta
      181 aactcggcat cgacaacttt ctcgtcgcca ccggttgact tttcctgcgt accagcatca
      241 tcacccggag cattaccctg tacggagaat gccgcctggt gcttctgcac aacttcaccg
      301 agcgtctgcg ctgctgcttt catgcgatct gcatcgcgtg acatcagagc ctcttttgcc
      361 ttagctactg caccttcaac ttctgacttg atacccgcat cgagtgctgg tgcgtcagct
      421 accatctttt ccgtcgtgta gatgatatga tcgagatcat tgtatgcagt cgctgcttcg
      481 cgtgccttcc tgtcctcttc tgcgttcgcc tctgcatctt ttaccatttt ctggatatca
      541 gcttcggaca gcgagctcga ggcttcgatc ttgatcttct gctctttatt ggtcttcaga
      601 tctttggccg atacgtgcac gatgccgttc gcatcaatgt caaaagtaac ttcgatctgc
      661 ggattgccgc gcggggcagg gggaatctct gacaactgga aacgacccag tgtgcggtta
      721 tcctttgcca gctcacgctc accctgcagc acatggatat ccaccgctgt ctggttgtct
      781 gccgcggttg agaaaatttc tgactttttg gtcgggatcg tggtgttacg ggtgataatc
      841 ttcgtcatca ctccacccag ggtttcaata ccgagagaga gcggggtgac atcgagcagc
      901 aggacgtcgg tgacttcacc ggcgagcact ccaccctgga tggcagcgcc gatcgcaacg
      961 gcttcatcag ggtttactga cttgtcgggt tctttaccga acagttcttt cactttttca
     1021 cgaacagccg gaatacgggt cgaaccaccg acaaggatca ccttgttgat ttcattcagt
     1081 gaataacctg aatcagcaat cgccttacgg cagggttcaa ccgagcgctc aacgagcgag
     1141 gctgtcagct ggttgaattt tgcccgggtg agcgtcatgt tgaggtggcg tggtcccgaa
     1201 gcgtccatcg tgatgaacgg gatgttgata tctgatgttt ctttgctcga aagctcgatc
     1261 tttgcttttt ctgctgcttc ctgcagacgc tgcagagcca tcttatcttt gccgacgtca
     1321 attgcagttt cctttttgaa ttctgcaaca atccagtcga taatcgcctg gtcaaagtcg
     1381 tcgccaccga ggtgtgtatc tccgttggtt gctttcactt caaacacgtt gtcggcaagc
     1441 tccagaatcg agatatcgaa tgtaccgccc ccaaggtcgt agaccgcgat aacttcacct
     1501 gacttgtcac ggtcgagacc gtaggcaagg gcagccgctg taggttcgtt gataatccgt
     1561 acgacatcaa ggccggcaat acggcctgcg tctttggtcg cctggcgctg ttcgtcatta
     1621 aagtaggcgg gtaccgtaac cacggccttc tccacttttg taccgagata ctcttcagct
     1681 gtctgcttca ttttcatcaa aacgcgcgca gacacttctt cgggacgcat gtctttttcc
     1741 cgggtttcaa tcagcaccga cttgcctgtg ccttctttta ccttataagc gactttgccg
     1801 atttctgaag tcacttcgct gaagcggcga ccaacaaagc gcttcactga acgcagagtg
     1861 ttcaccgggt tggtgaccat ttggttcttg gcgacctgcc ctaccagggt ttcacctttc
     1921 tctgtaattg cgacgatgga tggggtagtc cttgtacctt cgctgttggc gattactacg
     1981 gcttcaccac cttccatgac ggcgacgcac gagttcgtcg taccaaggtc gattccgatt
     2041 attttttctt ttgccatttc tttctcctta tatctctgtg agtagtgcct gtgacaccag
     2101 tattaagatt ccttgctctc cgttgcacgg ggtttagtta ccacaaccct tgcaggccga
     2161 atcacccgcc cttcgatgcg gtagcctttc tgaaagacct gcgacacggt ttctttttcc
     2221 acatctgcct cgacaaggct gagcgcctcc atgaggttag gatcaaattc agccccaaca
     2281 ggattcagtt cttcgactcc ggactctgta aagacccgcg taatctgctt atgcaccagc
     2341 tgtacaccgt cgacgatcgc cttgatgccg gcatctgctt cagccttggc taaataagcc
     2401 gcaaaaaact ggtcgaaggc atcgagcgca ggcaacagat cagtcagcaa gcgagcgctc
     2461 gattgtgcag cctgttgcgc tttttcctga acggcacgct tacggaagtt tgaaaactct
     2521 gcgcgctcac gctgcaggtc ggcggtgatc gattccagtt gctgccgtag ttcagcggtt
     2581 gcatccattg tgccggccgg cgagcccgcg gcagcagcct ccatcgacgc tatctcttct
     2641 gcctctgaag caaagtgttt tgcattgtca ttattcgttt gttccgtgct ttcgctcatt
     2701 gtttcctctt tggtttaggc tgtgccttgg cttttgctac agcttccggc agctctttac
     2761 gggcggttat ttcaatttcg ttaacgcgca gctcatcacc ctgcagttca atattgtttt
     2821 tttcactcag cacattagaa aggatctgtg aactgtaatc cagcgctgga atgatctttt
     2881 cgtagggcat acgattggcg ccgatcacac ccagtgcccc gatactcttt ccactgagcg
     2941 tgtagccttt accaagaatc gttatacccg aaagtatctc cccatcgatt tcgagcccga
     3001 cctgcgagcc aacctgcggc tggttcttca tgcgttcaaa caccgatgcg atataatctt
     3061 tattggccag ctgcgaaata attcgttcgg cttttcgccg gtcgggggtc aggtcgtaga
     3121 tcttgcggtg caggttagca tcaccttcaa tatgaatcgt ctggtccggc ggattataga
     3181 cgattgcctg cgtcaccgcc atgccaatgt cagagagctc cccgaggtct gccagtgaag
     3241 cgcgcaggaa ttcaagccct cgttcctgca cgtcgccgat ttcatgaccc ttaagattct
     3301 ggttcaggta gcgactgatc ttgtagagct cttcctggtt aacagaacgg tcgagtttga
     3361 tcttcttttc ataaatggct cccgatgaac taatcatgat catgagcacc aggctttcca
     3421 tgacagagac cagctcaata cgcttgatgc ttgtgcccac gctgttggga gccagtacga
     3481 tacctgcaaa atttgacgtc tgggccagca tatgcgcggt cgttttgagc aactggtcca
     3541 gctgcagtgc agcggagcga taaagatctt cgagttccgc tttctggtta agggtaatct
     3601 cgaacaggtt cacgaggtct gcgagataga gctggaatgc gcgatcagta ggaatgcgac
     3661 cagctgaagt atgcggtgaa ctcagcagcc ccatctgctc taatacagac atctcttttc
     3721 tgactgtagc aggagaaaat gccatactga atttctctac cagcacctgc gaacccacgg
     3781 gttcaccact ggctacgtac tcttttacaa gagcccgaag tatttcacct tgcctgtctg
     3841 tcatatgcag cgaccgtttt ttggtgatag cactctctgt caaagagtgc tatcagtagg
     3901 tatgatatgc aacaagagtt gaagcggcaa caaaaacata attgtgattt aggagcgtat
     3961 ctgaaaaaag aaggtctttc gacctagaaa acactttttg tttgacgctc tgttgagcgc
     4021 tcctaaagtg gcgctccgcc gcggcacctt cgggtggtgc agcagggcct tccggcgcgt
     4081 atggcgcgcc ccaaatgggt taaacggaag ttgatctgt
//
LOCUS       DAOQJF010000297         4107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13276_length_4107_cov_3.568608, whole genome shotgun sequence.
ACCESSION   DAOQJF010000297 DAOQJF010000000
VERSION     DAOQJF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13276_length_4107_cov_3.568608"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..784
                     /locus_tag="PLF85_13630"
     CDS             170..784
                     /locus_tag="PLF85_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20758.1"
                     /translation="MVDVLDESRRYFEARLGDCWHAPHLHTEQIRVYEQYLSIVRSSR
                     DAADYTQKLGPLASMFAVARAEQLDKYSNLVRLYQNLGNPDKLKAALERYETAKRAQN
                     HGDLVGLMTRAQDAAALTEGQGNEKLNLAVTLLQTVVAYAAYPADQDRSGKVAGILET
                     YTALKKLDPLFTWQSYAQTPYSYVVFFDAPRMQRLGQIFEQVKA"
     gene            812..1864
                     /locus_tag="PLF85_13635"
     CDS             812..1864
                     /locus_tag="PLF85_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20759.1"
                     /translation="MDEGSRGMLDSYKSGIDNYVNRLGKDHPKIVPLLALFAEFEALG
                     EKCSDFGALHAAASEQNFYGRLTNLMTEAAMAQPGEGKSNEPTVAQASQGYHAAYDSL
                     SAEIKAGPTGKAYERIFAIEKESASALQFMRRLAEEHLLVDISRVQLIAEFKRGQQEI
                     KNTAKHTGGGGISVPATEAYLKATIESMEQAKSITEVEYLAVARAEIAHMATLWDTGF
                     MNSLYHIFGNALTGYMMAATEENRQEVESSARFLGDFFGVDWQALHSVSRVWDFFSVT
                     LWPTVKENYAAKGITTAEGFRDYMKGYFDKAMKDQPPVAVNAANRTAWFWGRVVPLGD
                     VHKTLLNPPDVLSNKS"
     gene            1895..2410
                     /locus_tag="PLF85_13640"
     CDS             1895..2410
                     /locus_tag="PLF85_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20760.1"
                     /translation="MNFRSVLLPAGLILATAATRFLPHPDNFSPVMSVALFGSAVFVN
                     RFAGIALAMAAMAVSDYFLGLHSTLPFVYGAMALAGFLGFLLRENRGALRVLGTAFAG
                     SVVFFLVTNLGVFLMQDLYPHSVAGLVQCYVMALPFFQNSLAGDLLFTAVLFTVFHFA
                     ARDKSAAIAAA"
     gene            2407..3450
                     /locus_tag="PLF85_13645"
     CDS             2407..3450
                     /locus_tag="PLF85_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE20761.1"
                     /translation="MNSWRRALRFIATQQRGAKNARGINRKAVTIALGKERINADVYA
                     TGNAEAPTIFFLHGMSPLGISDPRQIVAAKALAAAGFRVICPELPEIRNLKVEAGSID
                     RFQKILRHLKDNRELCPTGRFALFAPSFSGAICLKVAADREVADSITTIAALGAFSRI
                     ERSLNHLMHDEAADPYARLVILANFLPRLKEGKRLGRYYLAAAHDNWQDTASRNPSLT
                     GFEPTDEKSKELRRLNATERKLIARIAEDTVFRIELGGRLLKVMQDTLDAYNVSEVAG
                     AIRAPVFLLHGAKDDVIPARESAELFPLLQEARLVVTPLMGHSHAAVSLRLIGDVWRL
                     VAGFAWFFRKAAE"
     gene            3447..>4107
                     /locus_tag="PLF85_13650"
     CDS             3447..>4107
                     /locus_tag="PLF85_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE20762.1"
                     /translation="MIAEVVGVTIARSGITVLEDVTLRIEAGQRILLTGENGSGKSSL
                     LMLLAAKLYPYNNAGERRYAWDSHADFRAARRYTALVSREEQLRLRQIHSASTVHEFL
                     LGHADGEDFLYRENTPAEAKYIGRLLSDWQIWHLADRKIRTLSLGELRLVQVVRAAMH
                     PRRLYLLDEIFSSLSDEIARRVSDWIESLPDSAAVVLTSHDEEIRRRFAPTRILHVAD
                     RR"
BASE COUNT          899 a         1132 c         1173 g          903 t
ORIGIN      
        1 cccgtgacct tccacgtgac aggcggatgc tctaaccaac tgagctacaa ccccaatgag
       61 gtgaaaaatt caccaaaaag gttgggcaga atttctaaac gaaacaggtt gtctacgaat
      121 tctgtaaaag attctgttga cactgcgatt tgtccctgtc gcatacgaca tggtagacgt
      181 tctcgatgaa tcccgccgtt attttgaagc gcggctgggt gattgctggc acgctccaca
      241 cctgcacacc gagcagatca gggtatacga gcagtacctc agtattgtcc gttcgagtcg
      301 cgatgcggca gattatacgc aaaagcttgg gccactcgcc agcatgtttg ccgtcgcccg
      361 ggctgaacaa ttggacaagt atagtaacct ggtgcgcctg taccagaacc tcggaaatcc
      421 cgataagctg aaagcggcgc ttgagcgcta tgaaacggca aagagggctc agaaccacgg
      481 cgatcttgtc ggtctcatga cacgggcaca ggatgccgcg gcgctcactg agggccaggg
      541 taacgagaag ctcaatctgg ccgtaacgct gctgcagacc gtcgtggcgt atgctgcata
      601 tccggcggac caggatcgct ctggtaaagt tgccggaatt cttgaaacct acacagcgct
      661 caagaagctc gacccgcttt tcacctggca atcgtatgca cagacaccct acagctacgt
      721 ggtatttttc gacgcaccgc gtatgcagcg cctgggacag atttttgaac aggtgaaggc
      781 gtaatgcgcg caagggcaaa ggagaacacg aatggatgaa ggcagccgtg gcatgctcga
      841 cagctacaaa tcgggcatcg acaattacgt gaaccggttg ggcaaagacc acccgaaaat
      901 tgtgccttta ctggcgctgt tcgcggagtt tgaagcgctg ggcgaaaaat gttctgattt
      961 tggcgcactc catgctgccg ccagtgagca gaatttctac gggcgcctga caaacctgat
     1021 gaccgaggcg gcgatggcgc agcccggcga aggcaagagc aatgaaccga ctgttgcgca
     1081 ggcttcacag ggctaccacg cagcctacga ttcgctttcc gcggagatca aggcaggccc
     1141 cacgggcaag gcttacgagc gcatatttgc tatcgaaaaa gaatctgcct cggcattgca
     1201 gttcatgcgg cggctggctg aagaacacct gctcgtggat atctcccgtg tgcaattgat
     1261 cgcagagttt aagcgaggac aacaggaaat caaaaacacg gcaaaacata ccggcggcgg
     1321 aggtatctcg gtgccagcaa ccgaggccta cctgaaggca actatcgaat cgatggaaca
     1381 ggcaaagagt atcaccgaag tcgaatacct tgccgtagcg cgcgccgaaa ttgcgcatat
     1441 ggcaacgctc tgggataccg gctttatgaa ctcgctctac cacatattcg gcaacgcgct
     1501 gacaggttac atgatggctg cgaccgaaga aaaccggcag gaagttgaaa gcagcgcccg
     1561 ctttctgggc gactttttcg gcgtcgactg gcaggccctg cattcggtaa gcagggtctg
     1621 ggatttcttt tcggtaacac tctggccaac tgttaaagaa aattacgccg cgaaggggat
     1681 cacgacagcc gaaggcttca gggattacat gaaggggtat ttcgacaagg cgatgaagga
     1741 ccagccgccg gtagcggtta acgccgcgaa ccggaccgcc tggttctggg gcagggtggt
     1801 gccgctcggt gatgtgcata aaaccctgct taacccgccc gacgttctct ccaataaatc
     1861 ttgacggagc acatcgccga ccggttctta cggtatgaat ttccgtagcg tactgctgcc
     1921 agctggcctg attcttgcca cagcggcaac ccgatttctg ccacaccctg ataatttcag
     1981 ccctgtcatg agcgttgcgc tctttggctc ggcggttttt gtgaaccgtt tcgctggtat
     2041 cgctctggcg atggcggcca tggccgtttc tgactatttc cttggcctgc actcaacgct
     2101 gccgttcgtc tatggcgcga tggccctggc tggtttcctg ggcttcctgc tgcgtgaaaa
     2161 ccgcggtgca ctccgcgtac tcggtacagc gtttgcgggc agcgtggtct tttttctcgt
     2221 taccaacctg ggagttttcc tgatgcagga tctttacccg cacagcgttg ctggccttgt
     2281 gcagtgttat gtaatggcgt tgcctttctt ccagaattca ttggccggcg acctgctgtt
     2341 caccgcggtg ctcttcactg tctttcattt tgctgcgcgt gacaaaagcg cggcaatcgc
     2401 agccgcttga attcctggcg ccgggcgttg cgcttcatcg ccacgcaaca gcgtggcgcc
     2461 aagaatgccc ggggcatcaa ccgcaaggcg gtgactatcg ctctgggcaa agagcgcatt
     2521 aacgcagacg tctacgctac cggtaacgca gaagctccca ctattttttt tttgcatggc
     2581 atgtcgccgc tgggtatttc agacccacgg cagattgttg cggcaaaagc gcttgcagcc
     2641 gcagggtttc gcgttatctg tcctgaactg cctgagatcc gcaacctgaa agttgaggcc
     2701 ggcagtatcg accgctttca aaaaatttta cgccatctga aagataaccg ggagctttgc
     2761 ccgaccggac gttttgcgct ctttgcgcct tcgttttcag gcgcaatctg tcttaaagtg
     2821 gccgccgatc gggaagtcgc cgacagtatc accacgattg ccgcgctcgg agcattttcg
     2881 cgcattgaac gttcgctgaa tcacctgatg cacgacgaag cggcagaccc ttacgccagg
     2941 ctggtcattc tggcgaattt tctgccacgc ctcaaagagg gtaaacgcct tgggcgctac
     3001 tacctggccg cggcgcacga taactggcag gataccgcgt cgagaaaccc ctcgctcaca
     3061 ggtttcgaac ctactgatga gaagagcaaa gagctgcgtc gtctgaatgc gacggagcgc
     3121 aaacttatcg cccgcatcgc agaggatacg gtgtttcgca tagagctggg cgggcgactg
     3181 ctaaaagtca tgcaggatac actggacgcc tataatgtct cagaagtggc gggtgctata
     3241 cgcgcgccgg tgttcttgct gcatggtgcg aaagacgacg tgataccggc gcgggaatct
     3301 gctgaattgt ttccgctgct gcaagaggcc aggttggtgg tgactcctct catgggccat
     3361 tcgcacgccg ccgtttcgtt acgtttgatc ggcgatgtct ggcgcctggt cgctggtttt
     3421 gcctggttct ttcggaaagc agcagagtga tcgccgaggt tgtcggagta accattgcgc
     3481 gctcgggtat taccgtgctc gaagacgtaa cgctgcgcat cgaagcgggg cagcgcattc
     3541 tgctcaccgg cgaaaatggc tcaggcaaga gctcgctact gatgctgctc gcggcgaaac
     3601 tatatcccta caataacgcg ggggagaggc gctatgcatg ggacagccat gctgactttc
     3661 gcgccgcacg acgttatacg gcattggtga gccgcgaaga gcaactacgc ctgcggcaga
     3721 tacattctgc cagcacggtg catgaattcc tgttagggca tgccgacggc gaagattttc
     3781 tctaccggga aaatacgcct gctgaggcaa agtatatcgg tcgactgctg tctgactggc
     3841 aaatctggca cctcgcagac cgaaagatcc gcacgttgtc gctgggcgag ctgcgcctcg
     3901 tccaggttgt gcgcgcagcg atgcaccccc gcaggctcta cctgctcgat gaaatattca
     3961 gcagcctctc tgacgagatc gctcgccgcg tttcagactg gattgaatca ctgccagatt
     4021 ctgcagctgt agtgctgact tcgcacgatg aagaaatccg ccggcgtttt gcgccgaccc
     4081 gcatactgca cgttgcggat cggcggg
//
LOCUS       DAOQJF010000298         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13278_length_4105_cov_5.600741, whole genome shotgun sequence.
ACCESSION   DAOQJF010000298 DAOQJF010000000
VERSION     DAOQJF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13278_length_4105_cov_5.600741"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /gene="nrfD"
                     /locus_tag="PLF85_13655"
     CDS             <1..725
                     /gene="nrfD"
                     /locus_tag="PLF85_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNE20763.1"
                     /translation="TWHHYEKMVMLLSALATPLVLSVHTIVSFDFATSVIPGWHATIF
                     PPYFVAGAIYSGFAMVLTLMIITREIFKLKEYITLKHLENMAIVILVTGMMVSFAYTV
                     EFVMAAYSASHIEQYAFINRLKGPFAWAFWIMFTCNVVSPHFMWFKSLRTNIKVLFVI
                     SIVVNIGMWFERFVIVIISLHRDYLPSSWDSYDPTWADYAVMIGSFGIFFTLFLMFIR
                     TIPSIALAEVKADEANAALSHH"
     gene            735..1286
                     /locus_tag="PLF85_13660"
     CDS             735..1286
                     /locus_tag="PLF85_13660"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNE20764.1"
                     /translation="MLSIAEIKRGLFSYEEVTGGFVASFKSAGDLLKAAEKTRQANVK
                     SFDCFSPCPIHGMDKAMGLNRSWIPVLTFIGGIMAVILGLSYIIYIDVFNWPIVFGGK
                     PFFSWPAYVPILFELAIYFASVFTVVGVLVLGRLGFMNRKPPAKGVTSDVFAIWLGDK
                     NLTRADVERILSGLNPQIEEVQV"
     gene            1283..1831
                     /locus_tag="PLF85_13665"
     CDS             1283..1831
                     /locus_tag="PLF85_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE20765.1"
                     /translation="MIKHSNKTVRIAVSALALAATVGCAETRREYHYFMDMHYSPGGD
                     TAKFDNIGQRPYDMYEPVGTVTYGGASAYPYKKDLDVDKATRELKAPATFDLKRGEKK
                     YQIYCAPCHGAQGNADGPVAKKFAGVRKLTNERADTFPMSKIYHIATAGQGAMAGYAP
                     QVQEQDRWNIAAYIKNELQKKK"
     gene            1852..3051
                     /locus_tag="PLF85_13670"
     CDS             1852..3051
                     /locus_tag="PLF85_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20766.1"
                     /translation="MKFALPEKNYKILWGMAGIGVISLAAGFILAADRAWLTLLSTGY
                     FVLSISLIGLLFAAIQFIAGAKWSVVLRRIPETLATPLYVAAAIIGVVAIGGALHLNH
                     VYEWADPKIVAGDELLQHKSGYLNMPFFLVRLVIYFAAWFFLSKLIRKTSAEQDTAKD
                     AATKSRLVKYSAIYTLLFAYSWLMASIDFVMSLQPHWFTTMFPVYTFANGWFGTIATM
                     IIILVTVQKHGGLKDVNEEHLHDLGKWQFMSVVFWAYIGFSMHMLTWYANMPEETYLL
                     ELRLKGVWKLFTVLLWLLHFLIPFFILLSRDIKRTPGRLVKVAWYILFVSFVDIIWVV
                     HGSALGHAGAENKVNSFPVGPLEIGAFLGAVGVFGIIFFKSFAKVNEAPTGDVDYEES
                     RHFHQTF"
     gene            3060..3133
                     /locus_tag="PLF85_13675"
     tRNA            3060..3133
                     /locus_tag="PLF85_13675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3092..3094,aa:Gly,seq:tcc)
     gene            3196..3792
                     /locus_tag="PLF85_13680"
     CDS             3196..3792
                     /locus_tag="PLF85_13680"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNE20767.1"
                     /translation="MFTGLIEETGTILAAHQVPGGRKFTISCHDILSDMKIGDSISVS
                     GACQTVEAFDGKSFTIFSIPETLSVTNFGDFSAGTVVNLERAMRLGDRIGGHLVQGHV
                     EATATVKSVSEGEIRNIVLHYDSPYILPKGSVTLNGISLTVMERLPGGDFRVQIIPET
                     IRKTDIGTWTAGKRINIEVDYIIKSLDLIRRQQSGDRG"
     gene            complement(3789..4064)
                     /locus_tag="PLF85_13685"
     CDS             complement(3789..4064)
                     /locus_tag="PLF85_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNE20768.1"
                     /translation="MPRIAQIYIRQMRFDDARQKLLREIERHFFAGESEVEIVHGIGD
                     YILRKMAEQELSKLDYVRMGDSYHTNPGSLRVTLLTPDPGLLDSYRG"
BASE COUNT          936 a         1078 c         1041 g         1050 t
ORIGIN      
        1 agacatggca ccactatgag aagatggtga tgctgctttc tgcgcttgca acaccgctcg
       61 tgctctcggt tcacacaatt gtttcttttg actttgccac ctcggtaatc ccaggctggc
      121 acgcgaccat ctttcctccg tacttcgttg cgggtgcgat ctactcgggc ttcgcgatgg
      181 tactgacgct gatgatcatc acgcgtgaga ttttcaagct gaaagaatat atcaccctga
      241 agcaccttga aaatatggca atcgtgattc ttgtgaccgg tatgatggtt tcatttgcct
      301 atacggtaga attcgtgatg gctgcctact ctgcttcgca catcgagcag tatgcattca
      361 tcaaccgcct caagggcccc tttgcctggg cattctggat catgttcacc tgtaacgtgg
      421 tttcaccaca cttcatgtgg tttaagtcac tgcgcacgaa catcaaagtg ctctttgtca
      481 tctcgatcgt ggtcaacatc ggtatgtggt ttgagcgctt cgtgatcgtg atcatctctc
      541 tgcaccgcga ctacctgcct tcaagctggg attcatacga tccgacctgg gctgactacg
      601 cggtcatgat tgggagcttt ggaatattct tcacactgtt cctgatgttc atccgtacga
      661 ttccttctat tgcgctggct gaagtcaagg ctgacgaagc caacgcggcg ctcagccacc
      721 actaaagagg taatatgtta tcaatagctg aaattaaacg cggcctcttc tcttacgaag
      781 aagtcaccgg cggtttcgtg gcttccttca agtctgcagg tgacctgctg aaggcagctg
      841 agaaaacacg ccaggcgaat gtgaagagtt tcgactgttt tagcccatgc ccgatacacg
      901 gcatggacaa agccatgggt cttaaccgtt cctggattcc ggtactaact tttatcggcg
      961 gcattatggc cgttattctg gggctgtctt acatcatcta catcgacgta tttaactggc
     1021 caatcgtttt tggtggaaag ccctttttct cttggcctgc ttacgtaccg attctcttcg
     1081 agctagcgat ttacttcgcg tcagtcttta ctgttgttgg tgttctggtt ttgggtcgtc
     1141 tgggtttcat gaaccgcaaa ccgccagcaa agggagttac ttcagacgtt ttcgcaatct
     1201 ggctgggtga caaaaacctt acccgtgccg atgtggaacg tattctctca ggtctgaatc
     1261 cacagattga agaggtacag gtatgataaa gcacagcaat aaaacagtac gaatcgcggt
     1321 ctcagcctta gcgctggccg caacggttgg ctgcgccgag acgcgccgtg agtaccacta
     1381 cttcatggac atgcactact caccgggcgg tgacaccgct aaattcgaca acatcggcca
     1441 gcgtccctac gacatgtacg agccggtggg tacggtcacc tatggtggcg cgagcgcata
     1501 cccgtacaag aaggatctgg atgtcgacaa ggcgacccgt gagctcaagg cacccgcaac
     1561 cttcgatctg aaacgcggcg agaagaaata ccagatttac tgcgctcctt gccacggtgc
     1621 tcaaggcaat gcggacggtc cagttgcgaa gaagtttgct ggcgtgcgca agctgactaa
     1681 tgagcgagcc gacaccttcc ccatgagcaa gatctaccac attgctacgg cgggacaagg
     1741 tgcaatggct ggttatgctc cgcaggttca ggaacaggac cgctggaaca ttgctgccta
     1801 catcaagaac gagttgcaga agaagaagtg acggctgcca cggagaaaaa catgaaattt
     1861 gcattacctg aaaagaatta caagatactc tggggtatgg caggcattgg cgtcatatct
     1921 ctggcagcag gattcattct cgctgctgac cgcgcctggt tgaccctgct gagcacgggc
     1981 tactttgtcc tgtcgatttc actgatcggc ctcctgtttg cggctatcca gtttattgcc
     2041 ggagccaaat ggtcggtagt actgcgtcgt atacctgaaa cgctggcaac acctttgtac
     2101 gttgcagcgg ccatcatcgg tgtagtggcc attggcggcg cgctgcatct caaccatgtc
     2161 tatgaatggg cagatccaaa gatcgtcgca ggagacgaat tgctgcagca caagagcggc
     2221 tacctcaata tgccattctt cctcgttcga ctggtgatat attttgctgc atggttcttt
     2281 ctttcgaaac tgatccgtaa gacttctgca gaacaggata cggcgaaaga cgcggcgacc
     2341 aagtcacgcc tggtcaaata ctcagctata tacacgctcc tcttcgccta ctcatggctc
     2401 atggcatcta tcgacttcgt gatgtcactg cagccgcact ggttcacgac aatgttcccg
     2461 gtgtatactt ttgctaacgg ctggttcggt accatcgcaa cgatgattat catcctggtg
     2521 accgttcaga agcatggtgg tttgaaagac gtaaacgaag aacacctgca tgacctgggc
     2581 aaatggcagt tcatgtcggt tgttttctgg gcgtacattg gcttcagcat gcacatgctg
     2641 acctggtacg cgaatatgcc agaagagacc tacctgcttg agctgcgcct caagggcgta
     2701 tggaaactct tcacagtact gctctggctg ctccacttcc tgatcccttt ctttatcctg
     2761 ctgtcgcgcg acatcaagcg cactccaggc cggcttgtta aagtagcctg gtacatcctg
     2821 tttgtcagct ttgtcgacat catctgggtt gtccatggtt cagcgctggg ccatgcaggc
     2881 gcagagaaca aggtcaacag cttcccggta ggccccttgg aaatcggcgc tttcctcggc
     2941 gcggtaggag tatttggtat tatcttcttc aaatcttttg cgaaggtaaa cgaagctcca
     3001 accggagatg tggattacga agaatcccgc catttccacc aaacgttcta gaaacttacg
     3061 cgggcatggt gtaacggtag cacaaaagcc ttccaagctt ttagagaggg ttcgagtccc
     3121 tttgcccgct ccatttttta aagggaactt ccatagagat aacatctctt cggaagttcc
     3181 actgttcaaa taaccatgtt cactggcctt attgaagaaa ccggcacaat cctggcagcc
     3241 catcaagttc ccggaggcag gaagtttaca atttcatgcc acgatattct gagcgacatg
     3301 aagatcggcg acagtatctc tgtttccgga gcctgccaga ctgttgaagc ttttgatggc
     3361 aaatcgttca cgatattctc gatacccgaa actctttcag tcacgaactt cggcgatttt
     3421 tccgctggaa cggttgtgaa tctggaacgc gccatgcggc tgggtgaccg tatcgggggc
     3481 cacctggtac aggggcacgt agaggccacc gcgacggtca aatctgtgtc tgaaggagaa
     3541 atccgcaaca ttgtgctgca ttatgactcg ccgtatatac tgcccaaggg atcagtaacc
     3601 cttaacggca tcagcctgac cgtcatggag cgtcttccgg gtggtgactt cagggtgcag
     3661 attattccgg aaacgatccg caagacagat attggcacct ggacagcggg caagcggatc
     3721 aatattgaag tggattacat catcaaatca ctcgatctga tacgccggca acaatccggt
     3781 gatcgcggtt aaccccggta gctgtcgagc aaccccgggt ctggtgtcag cagcgtcacc
     3841 ctcaatgaac cgggattcgt gtggtaactg tcccccattc gcacatagtc gagcttgctg
     3901 agttcctgtt ctgccatttt gcgcagtata tagtcgccta taccatgcac aatctcgact
     3961 tcgctttcac cggcgaagaa gtggcgctcg atttcccgca ggagtttctg ccgcgcatcg
     4021 tcaaagcgca tctggcgaat gtagatctgg gcaatgcgcg gcatgttata tctggtgctc
     4081 cggcacacct gttcccaatg ctatc
//
LOCUS       DAOQJF010000299         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13280_length_4105_cov_4.509383, whole genome shotgun sequence.
ACCESSION   DAOQJF010000299 DAOQJF010000000
VERSION     DAOQJF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13280_length_4105_cov_4.509383"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PLF85_13690"
     CDS             <1..1203
                     /locus_tag="PLF85_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20769.1"
                     /translation="LVARRGGISTYQEPLVLISGVNLRGGYSATFLDAEKDMSYGNQT
                     RVAIPGTLLFALNITGTTTVEGFHLSATDSASAVGLISGSNNNLILQYNRLDTSVNQP
                     GPSYGLLVQNSGTTHANGPIIRHNAILSGNVTTPSSITAGVRLENSALQLRNNYIKSG
                     TIVNGIANFLSLASGLLVVNSNPLAVNNVIISNSVASGSDAWSIGVYHFTGTGGTYSN
                     NTIATLWTSGNTYAVALNGGTGMPLFANNIFFNAGGGRVFYEWNATDNPVSLHNNAYV
                     GGGFFYRDNGASDRADGDVNNANNTNQGTAATVSGNLRTAVGCAPFVNYGGDDWRLQQ
                     NSCTANEWRDLRYGGRNTSLSNCGTGSASCGSITDDLNSADRTAVNTGSSPLTNAAGY
                     SMGAYETD"
     gene            complement(1220..2119)
                     /locus_tag="PLF85_13695"
     CDS             complement(1220..2119)
                     /locus_tag="PLF85_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE20770.1"
                     /translation="MNLAGKTIAVTGASGMIGVYICRALLRRGAKVVGVVRNPEKAAF
                     LANEGVEFRRADLADRAALEAAFAGCDAVVSNAALYRLTALDWESNYRPNKEGTENVY
                     RAAAKNGIRRAVQISTIGLYQFSLFSEIDENSPQVNGEAREGGAYRATKQLSESLAWH
                     LCNANGIALTALRPSGVYGARDTNIMPYFAKLMKLPFLPFPGFAWPLVYAGDVADAVV
                     AALENDQSAGEAYNTTGDTSDFADFLEAWQRVSGRGRVFKITPSFGLRVSHAKASRDL
                     GFRNRPFDEALAEIFAEEPELLR"
     gene            complement(2136..2585)
                     /locus_tag="PLF85_13700"
     CDS             complement(2136..2585)
                     /locus_tag="PLF85_13700"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE20771.1"
                     /translation="MQIHTELEINAPAEEVWKVLVDFPQYGEWNPVITSIDGAPYVGE
                     KISFKIRAAQGVEIPIIACEILVANAAQGELRWRGPAIPLLDQVIAGEHYFIVQSAGE
                     GKSRFVHGENFTGLLAAPLEPLLQQRLTESYSEMNRALRARCEGRSR"
     gene            complement(2629..3204)
                     /locus_tag="PLF85_13705"
     CDS             complement(2629..3204)
                     /locus_tag="PLF85_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20772.1"
                     /translation="MLGIPLGLLAANAFEWYAHRYILHEAGRDKKGRAHYHWDHHREV
                     RRNNFREPMYDHLLGEEYDRHRFEIEALVKVSLAVSPLFPIAPLFTATLWYSAINYYY
                     CHRKSHDNPEWAREHLPWHVDHHLGRDQDMNWCVTKPWFDYIMGTRVYTGHSKTESNP
                     LGIPLPKELEDFLWSLVPRAQQEPARVSSAA"
     gene            complement(3282..>4105)
                     /locus_tag="PLF85_13710"
     CDS             complement(3282..>4105)
                     /locus_tag="PLF85_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20773.1"
                     /translation="SLKGSDLAGHTYTGQQLSPDGRYLLARGGNAVSVTPLFGEGIMQ
                     ATGQFRDCRWVSAAAVACIEGPYQKETRLVQLRLPQLRRSILVETQGEYLRELATDSV
                     RGSIAYATQAEKLVRFWLRSATEGRAKILGELPGHYVFALAVSSQGTVAARVASAERS
                     AELLLPQNIWFSGTRDHRQGLLLDLPALAHPGFFSGAGFQGVEAFDFSPDGAALAILM
                     SSRDGCRMADEGGNLACRLNVFTYEIPRGSVRQLTHFKASQLNGIAWRTWSRLTE"
BASE COUNT          922 a         1170 c         1153 g          860 t
ORIGIN      
        1 ctcgtcgcac gacgcggggg aatttcaact taccaggaac cattggtgct gatttctggc
       61 gtaaacctca ggggcggcta ctcggcaacg ttcctggacg cagaaaaaga catgtcgtac
      121 ggcaaccaaa cccgtgtcgc gataccgggc accctgctgt ttgcattgaa tatcactggc
      181 acaactacag tcgaaggctt tcatttatct gccacagaca gcgccagcgc agtcgggctc
      241 atttctggct cgaacaacaa cctgattctt cagtacaacc gtctcgatac ttcagtgaat
      301 caaccggggc cttcgtatgg tttacttgtg cagaacagtg gcacgaccca tgcaaacggc
      361 ccgatcatcc ggcacaatgc catcctgagc ggaaatgtta ccacgccaag tagcataacc
      421 gcaggcgtca ggcttgagaa cagcgcactg cagctgcgca acaactatat caagtcaggc
      481 acaatcgtca atggtatcgc caatttcctc tcactggctt ccgggctgct ggtagtaaat
      541 tcaaatccac tcgcggtaaa taatgtaatc atctccaact ccgttgcgag tggaagcgat
      601 gcctggagca ttggggtata ccactttaca ggcacaggag gaacttattc aaacaatacc
      661 attgcaaccc tgtggacgag cggaaatact tacgcggtgg cactgaacgg gggaaccggg
      721 atgccactct tcgccaataa cattttcttt aacgcaggtg gaggccgggt tttttacgaa
      781 tggaatgcga cggataatcc cgtttcgctg cataacaatg catacgtggg tggtggcttc
      841 ttctatcggg acaatggcgc ctctgaccgc gcagatgggg atgtgaacaa tgcaaacaac
      901 acgaaccagg gcactgcggc aactgtttca ggcaatttgc gcacggcggt tggctgcgcg
      961 cctttcgtga actacggtgg tgacgactgg cgtctccaac agaatagttg cacagccaac
     1021 gaatggcgcg acctgcgcta cggcgggcgc aataccagcc tttctaattg cggcacgggc
     1081 agcgcttcgt gcggaagcat aactgatgat ctgaattcag ccgaccgcac ggcggtgaac
     1141 acggggtctt caccactcac aaatgcggct ggctatagca tgggggcgta tgagacggac
     1201 tagctatgga attccatagc tagcgcagca attccggttc ttcagcgaaa atttcagcca
     1261 gcgcctcgtc aaacgggcgg ttgcgaaatc cgagatcacg gctcgctttt gcatggctga
     1321 ctcgaagacc aaaggatggt gttattttga aaacgcgacc gcgccctgag acacgctgcc
     1381 aggcttccag gaaatccgcg aagtcagaag tatcgccagt cgtattgtag gcctcgcccg
     1441 ccgattggtc attttcgagc gcagcgacca ccgcgtctgc aacatcacct gcatagacga
     1501 gcggccaggc gaaaccggga aaaggtagaa acggcagctt catcagcttc gcgaaatagg
     1561 gcataatatt ggtatcgcgg gcaccataga cgccagaagg acgcagcgca gtcagcgcga
     1621 tgccattcgc gttacagagg tgccacgcca gcgattcaga aagttgtttc gtggcgcggt
     1681 aagctccgcc ttcgcgcgct tcgccgttga cctgcggcga gttttcgtcg atttcgctga
     1741 agagtgaaaa ctggtaaagc ccgatcgtac tgatctgtac tgcgcgcctg ataccatttt
     1801 ttgctgctgc gcggtatacg ttctctgtcc cttctttatt cgggcggtag ttcgattccc
     1861 agtcgagagc ggttaaacgg tagagcgcgg cattggagac gacggcatcg caacccgcga
     1921 acgccgcttc gagagcggca cggtctgcaa ggtcagcacg gcgaaactca acaccttcgt
     1981 tagcaagaaa agctgccttc tcaggattac gcaccacacc gacgactttt gcaccgcgcc
     2041 tgagcagcgc gcggcagatg tagacaccga tcatgcccga agcgccggtg acggcgatgg
     2101 ttttaccggc caggttcatc tgctacgggt ttgcgtcagc ggctgcggcc ttcacagcgt
     2161 gcgcgcagtg cacggttcat ttcgctgtat gattcggtaa gacgctgctg cagcaatggc
     2221 tcgaggggag cagccagcag accggtaaag ttttcgccgt gcacgaaacg tgacttgccc
     2281 tcgcccgcgc tctgcacgat gaagtagtgc tcacctgcaa tcacctgatc gagcagcggt
     2341 atcgccggcc cgcgccagcg caactcaccc tgcgcagcat tcgcaacgag aatttcgcag
     2401 gcaatgatcg gaatctcgac accctgcgca gccctgatct taaaactgat cttttcgccg
     2461 acgtaaggcg cgccatcgat cgaggttatg accgggttcc attcgccgta ttggggaaaa
     2521 tccaccagga ccttccagac ttcttcggcc ggcgcattaa tttcgagttc ggtatgtatc
     2581 tgcatgccgg acttttgtgc agggcgtcat attgcgcaca gcacttaatc aagcagcgga
     2641 actcacacgc gcaggttctt gctgcgcgcg tggtacaagg ctccacaaga aatcttcgag
     2701 ctctttcggc agcggtatgc cgagcgggtt gctctcggtc ttgctgtgcc cggtgtacac
     2761 ccgtgtgccc atgatgtaat caaaccacgg tttggtcacg caccagttca tgtcctggtc
     2821 gcgcccgagg tggtggtcaa catgccaggg taaatgttcg cgcgcccact cggggttatc
     2881 gtgtgacttg cggtggcaat agtagtagtt gattgccgaa taccaaagcg tggctgtaaa
     2941 aagaggcgca atcgggaaca gaggtgagac cgcgagcgag acctttacca gcgcttcgat
     3001 ttcaaagcgg tggcggtcat actcttcgcc cagcaggtgg tcgtacatcg gctcgcgaaa
     3061 attgttgcgc ctgacttcac ggtggtggtc ccagtggtaa tgtgcacggc cctttttatc
     3121 gcggcctgct tcgtgcagaa tatagcggtg cgcataccac tcaaacgcgt tggcggccaa
     3181 aaggccaagg ggaattccca gcatggcttt ggatttccat agcaggcctg cggggtcaag
     3241 ctttaatgac gatgatgtca gttttcacag aagatactcc ctcactctgt caaccttgac
     3301 caggtgcgcc aggcaattcc gttgagctgg ctcgccttga agtgcgtaag ctgccgcacc
     3361 gacccgcggg ggatttcata agtaaagaca ttgagccggc aggcaagatt gcctccttcg
     3421 tctgccattc ggcaaccatc gcgtgaagac atgagtatcg caagtgccgc accgtcggga
     3481 gaaaagtcga aggcttcgac accttgaaac cccgcaccac tgaaaaaacc cgggtgagcc
     3541 agcgccggca gatcgagcaa caggccctgc cggtggtcac gcgtgccgga gaaccagata
     3601 ttctgcggca gcagcaattc ggcgctgcgc tcggccgaag cgactcgggc agcaacagtc
     3661 ccctgagaag aaacagcgag ggcgaagacg taatgccccg gcagctcgcc caagattttc
     3721 gctctgccct ccgtcgcact gcgcagccag aagcgcacca gcttttctgc ctgtgttgcg
     3781 tacgcgatgc tgccccgcac ggaatcagtc gcaagctcgc gcaggtattc tccctgcgtt
     3841 tcgacgagta tactgcgccg cagttgcggc agtctgagct gcacaaggcg ggtctctttc
     3901 tggtaaggtc cttcgatgca tgccacggcc gctgccgata cccagcggca gtcgcggaac
     3961 tgcccggtgg cctgcattat gccctcgcca aaaagcggcg tcacagatac ggcatttcca
     4021 ccacgcgcaa gcaaataccg gccatcggga gagagctgtt gccccgtata cgtatggccg
     4081 gctaggtcag aacctttcag ggacc
//
LOCUS       DAOQJF010000300         4097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13314_length_4097_cov_5.098219, whole genome shotgun sequence.
ACCESSION   DAOQJF010000300 DAOQJF010000000
VERSION     DAOQJF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13314_length_4097_cov_5.098219"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLF85_13715"
     CDS             complement(<1..225)
                     /locus_tag="PLF85_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNE20774.1"
                     /translation="MTQLIQTIAATQAEWTGLVLRLALAAMILPHGAQKLLGWFGGYG
                     FKGTMGYFTGTAGLPAILAFLVIIGEFFGGL"
     gene            398..868
                     /locus_tag="PLF85_13720"
     CDS             398..868
                     /locus_tag="PLF85_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE20775.1"
                     /translation="MKHDTAAIVAWRNLLAYQAEATFRIERELKHAGCIPLAAYDVLI
                     ELETSDGKRLRMYDLSKACVLTKSGATKIVNSLEKQGYLRREKCPSDLRGLFAAITAK
                     GSAAVRKAWPVYRRCIEDFFTSGVTAAELKHLSEILPKLRAKLPDNFMESACNS"
     gene            complement(935..1291)
                     /locus_tag="PLF85_13725"
     CDS             complement(935..1291)
                     /locus_tag="PLF85_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE20776.1"
                     /translation="MERCKVLINENAEKGYATAIFIGALNLFTAKQARAELEPLLARE
                     RLRVIIDLEDLETIDSSGIGALVNFILAIRKHEDARVVFTQPRSAVMHVFEVTKLISF
                     FTIIASISEAEQYFSA"
     gene            complement(1325..2230)
                     /locus_tag="PLF85_13730"
     CDS             complement(1325..2230)
                     /locus_tag="PLF85_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE20777.1"
                     /translation="MAVISRTQFISLSAAAALHYPFFPLLSKKKQAMTATILSTGEIL
                     PRIGLGTYQIFEAVEPQHAAALLGEFYSLGGRLVDSSPMYGEAEAVTGSASVQLGLNA
                     QLFLATKVWTSGRERGVSQIKESLRLLGRQKLELMQIHNLVDWKTQIKTLRTYKEAGI
                     FRYIGLTHYQVSVFRELARILKNEAVDFLQIPYSIAETAAEERLLGIAHERQVAVIAN
                     EPFAQGRLFRQVKGKPVPAWAVERGMKSWAQYFLKFILSEPRIQFVIPATRRLEHLRD
                     NMSAGVGYLPDASERQRMLADFRNL"
     gene            2363..3325
                     /locus_tag="PLF85_13735"
     CDS             2363..3325
                     /locus_tag="PLF85_13735"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNE20778.1"
                     /translation="MKKATEMPEQGANVVFSEGDRVTGRSKIGSWFSGDKMFIAMHFI
                     PLAAIWTGVTWKAVWLGVALYWIRMFFVTAGYHRYFAHKSFETSRIFQFILAFGAETT
                     GQKGVLWWSGHHRDHHKYSDTEKDVHSLKVHGFYTSHIGWIFDKNNKRVPLENIQDFA
                     KYPEIRFISKHDWIAPWALGVASFLIAGWPGLWIGFFLSTLITYHSTFTINSLTHKWG
                     KQRYATGEESRNHWLLALTTMGEGWHNNHHYYQASARMGFYWWEIDLTYYVLRFLSFF
                     GIVWNLKGVPKAVREKTMKAQAARTEKAAQPAITPVANSLEATG"
     gene            3446..>4097
                     /locus_tag="PLF85_13740"
     CDS             3446..>4097
                     /locus_tag="PLF85_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE20779.1"
                     /translation="MPLRDRILKSLTFLSIAGSIILGNWLHPYLEGQPVNDPLIIRIA
                     IAAFSVCFFVLSLTAASVALRERLYYVLYFPITGQGLYLSATNSLMHSYAFGMAIITT
                     VVITSAESRRFLVAYVPFTIVGTVAAGLAASEPRVTRSVYLFANITLVLLLYSLLNSK
                     LALLTSLRRATEKERAASLAKTAFVARMSHEIRTPMNGILPVIDLLRKSSDEAEKAR"
BASE COUNT          953 a         1131 c         1085 g          928 t
ORIGIN      
        1 caggccgccg aagaattcgc ctataattac gagaaatgcg aggatggcag gcagacccgc
       61 ggtaccggtg aaatagccca ttgtaccttt aaagccgtaa ccgccaaacc agcccagaag
      121 tttctgcgca ccgtgtggta aaatcatggc tgcaagcgcg aggcgcagca cgaggcccgt
      181 ccattctgcc tgcgtggcag cgattgtctg aataagttgt gtcatgttat ttgctccgtt
      241 tattttattg agccctcctc ttctgtttca ttgcgacgct tatgcgcatc gcgatgaaac
      301 agcccagcag gcgccctatt agttgattac tcaaccaaca agaagaagct aacccgcttt
      361 aattgactcg tcaacaaaaa gttagtaatt tgaccgcatg aagcacgata ccgctgccat
      421 tgtcgcctgg cgcaacctgc tcgcctacca ggcagaggcg acttttcgca tcgagcgcga
      481 actcaaacac gccggctgca ttccactggc ggcatacgac gtgctgattg agctcgaaac
      541 ctccgacggc aaacggctgc gcatgtatga tctttcgaaa gcctgcgtat taaccaaaag
      601 cggagccacc aagatcgtca acagcctcga aaaacaggga tacctgcgcc gcgagaaatg
      661 cccgagcgac ctgcgcggtt tgtttgccgc gatcacggca aagggcagcg cagcggtgcg
      721 caaggcctgg cctgtctacc gcaggtgtat cgaagacttc ttcacctcgg gagtgaccgc
      781 cgcagagcta aagcaccttt cggaaatact accgaaactc agggccaaac tgccggacaa
      841 cttcatggag tccgcctgta attcgtaaga agatacgctc gtgaaaaggg gatgactcga
      901 gcgaatttgc gattctgcca aggctggcgg gcccttaagc ggaaaaatat tgctctgctt
      961 cagaaatgct tgcgataatg gtgaagaatg aaataagctt tgttacctca aaaacgtgca
     1021 tcacagctga acgcggctgg gtaaagacca cgcgcgcatc ttcatgcttg cgtatcgcca
     1081 ggatgaagtt caccagcgct ccgatgcctg aagaatcaat ggtctccagg tcttcaagat
     1141 cgatgatcac gcgcaggcgt tcccgtgcaa gcaatggctc gagttcggcg cgcgcctgtt
     1201 tcgcggtaaa caggttgagc gcaccgatga aaatcgcggt agcgtaaccc ttttcggcat
     1261 tttcattgat cagtaccttg cagcgctcca cgatggcaat gtagagacgc gattaatcgc
     1321 gtctctacaa attcctgaag tctgccagca tacgttgacg ctcgctggca tcgggcaaat
     1381 accccacgcc ggcagacatg ttgtcgcgca ggtgttcgag ccggcgggtt gccggtatga
     1441 caaactggat acgtggctca gaaagtatga atttcaggaa atattgcgcc cagcttttca
     1501 tccccctttc gacagcccac gcgggcacag gtttaccctt tacctggcga aatagccgtc
     1561 cctgcgcaaa cggttcgttg gcaatcacag cgacctggcg ctcgtgcgca atacccagca
     1621 ggcgttcttc cgctgccgtt tctgcgatcg agtacggtat ctgcagaaaa tccaccgctt
     1681 catttttgag aatgcgcgcg agctcgcgaa aaaccgatac ctggtagtgc gtaagaccga
     1741 tgtaacgaaa gatgcctgct tctttatatg tgcgcagcgt tttgatctgg gtcttccaat
     1801 cgacgaggtt gtgtatctgc atcagttcga gcttttggcg tccgagcagg cggagtgatt
     1861 ctttgatctg agacacgccc cgttcgcggc ccgaagtcca gaccttggtg gcaagaaaaa
     1921 gctgcgcgtt gaggccgagc tgcaccgaag cactgccggt aacggcttcg gcctcaccgt
     1981 acatgggcga cgaatcgacc aaccggccgc cgagagagta gaattcgcca agtagagccg
     2041 cagcatgctg cggctctact gcttcgaata tttgatatgt tccgaggccg atgcggggta
     2101 atatttcgcc ggtggaaaga atcgttgcgg tcatggcttg tttctttttg gagagcagcg
     2161 ggaaaaacgg ataatgcagt gccgcagcag ctgaaaggga aataaattgc gtgcgggata
     2221 tgactgccac acggttattg tagagacgcg attaatcgcg tctctacgaa ttacagatca
     2281 tccaattctt cgcgtctcta caaatcctgg ggaaatccgc gtttttttgc aaccgacctc
     2341 gcagaccccg catcgcacga acatgaagaa agctacggaa atgcctgaac agggcgcaaa
     2401 cgtcgtcttt tccgaaggcg accgggttac aggccgctca aaaatcggtt cgtggttcag
     2461 cggcgacaag atgttcatcg ctatgcactt tattccgctc gcggccatct ggacgggtgt
     2521 tacctggaag gcagtctggc tgggagttgc gctctactgg attcgtatgt tcttcgtgac
     2581 tgcaggctac caccgctatt tcgcgcacaa gtcgtttgag accagccgca ttttccagtt
     2641 tattctcgca ttcggcgctg aaactaccgg ccaaaaggga gttctctggt ggtctggtca
     2701 ccaccgcgac caccacaagt atagcgacac tgaaaaagat gtgcactcgc tcaaggtaca
     2761 tggcttttac acctcgcaca tcggctggat cttcgacaag aacaacaagc gcgtaccgct
     2821 cgaaaacatc caggactttg cgaagtaccc cgagatccgt tttatttcta aacacgactg
     2881 gatcgccccg tgggcgctcg gcgttgcttc gtttctgatt gcgggctggc cagggctgtg
     2941 gattggcttt tttcttagca cgctgattac gtaccactca accttcacta tcaactcgct
     3001 gacgcacaaa tggggcaagc agcgctacgc taccggcgaa gaatcgcgca accactggct
     3061 cctcgcgctc accacaatgg gcgaaggctg gcacaacaac caccactact accaggcatc
     3121 ggcgcgcatg ggcttctact ggtgggaaat tgacctcacc tactacgtac tgcgtttctt
     3181 gagtttcttt ggcatcgtct ggaacctgaa gggagtgccg aaggctgtgc gcgagaaaac
     3241 aatgaaagcc caggcagcgc gtactgaaaa agcagcacaa cctgcaatta cgcccgtggc
     3301 caattcactc gaagcgaccg gttaactttt taccgcgacc aggtctgtct cgcgcagaac
     3361 tggtcgcagt tgtactctgt tttctctcaa tattcaagag cgttagcggc ttgaccgcac
     3421 gtgggtaaat aggccgaata aaaagatgcc tcttcgggac agaattctca agagcctgac
     3481 gtttctctcg atcgctggca gcatcatcct ggggaactgg ctgcatccgt acctcgaagg
     3541 ccagccggtt aacgatccct taatcatacg cattgcgatt gctgcctttt cagtgtgctt
     3601 cttcgtgctg tcgctcacgg cagcctccgt ggcgttgcgt gagaggctat attatgtact
     3661 gtatttcccg atcaccgggc agggactgta cctttctgca accaacagcc tgatgcacag
     3721 ttacgcgttc ggcatggcga tcatcaccac ggtcgttatc acctctgcag aaagcaggcg
     3781 ctttcttgtg gcatatgtac cctttactat cgtgggaact gttgcggcag gccttgccgc
     3841 ctctgagccg cgagtcacgc ggtcggtcta cctctttgcc aacatcaccc tggttctgct
     3901 gctgtattcg ctgctgaaca gcaagctcgc actgctaacg tcgctcaggc gggcaactga
     3961 gaaagaacgg gcagcttcac tggcgaaaac ggcgttcgtc gcgcgcatga gccatgagat
     4021 acgtaccccg atgaacggta ttctgccggt gatcgacctg ctacgcaaga gcagcgacga
     4081 agcagagaag gcgcgct
//
LOCUS       DAOQJF010000301         4085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13361_length_4085_cov_5.566998, whole genome shotgun sequence.
ACCESSION   DAOQJF010000301 DAOQJF010000000
VERSION     DAOQJF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13361_length_4085_cov_5.566998"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLF85_13745"
     CDS             complement(<1..390)
                     /locus_tag="PLF85_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNE20780.1"
                     /translation="MHTLVKYFRTMPPVALIGASNDSTKYGNIIFKDLKSKGYTVYPI
                     NPKATSIEGVPALHSLEELKEKTNVDLLVYVIPPKLVLESLHKAKELGYKKVWLQPGA
                     ADAAVAEFLDTHDFTYIIGACVMVESRD"
     gene            complement(472..2064)
                     /locus_tag="PLF85_13750"
     CDS             complement(472..2064)
                     /locus_tag="PLF85_13750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE20781.1"
                     /translation="MFKKSWIVFLSLSLALAAQQDTEKKEAPPADAAKTETADKPAPA
                     AQPAEASFTIGGVQLTIQDAINIVLEKNLTLQAAKYDVIMSDTAARKLEKKYAPIISA
                     EGQHLNFSDAPLASNSKGYQYNAALGIQKLFVTGTTVGGGYRYQQLHNEAGITSFAGM
                     NFPTPQSTNTFNGYFINVQQELLKNSFGYADRKMDKIAKKQAESQRAYTVNLLSGLVV
                     QALTDYWQVTIQKFALENAKLEEKSNRQVRAIVARNVSYGLGESYDLNNYNARVANSV
                     AKVAMTEQSLRNATRKLLRTVNMPVDTKIEGITNLIDTLPNLDANAALKAAFEKRVDF
                     KNARNEMEIAEMQSDLYGNQALPSLNAYFNLVSQGTNQMIMFPGFPSAGSLQSPQWQV
                     GVKATYPLWDEEVKVNQRNAGMQLTQSRIKLQNIEQEIRDEVLTRLENVRLTFEIFQN
                     SRTSRKESEAFYNRMLARTRTGKLNFQLVGQALENMVANRQRELESLVNYNIALLQFD
                     LAKNEIFERYKVDVEKILEKVK"
     gene            2971..3663
                     /locus_tag="PLF85_13755"
     CDS             2971..3663
                     /locus_tag="PLF85_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20782.1"
                     /translation="MDQNVKLRNLLFGSVIALLLSSIVLAIVHPAKPVKAMQQGVNMP
                     VVGLEMAWTTAEVWDILGDPQSDAGQEARRAFALGTWLDFGYIFCYSLAYLFLALLLV
                     HRHSAGRIWIVLSALVICVTAFGDVMENRAIFSILDSGTQSLAAEHVDALVVFTRLKW
                     LFLGINGLPAAVLLRREGKRGLSFILTAAFAFGALGVLKQYAIELMTIFLAFFWAYLL
                     VKLLPLKNRWWA"
     gene            complement(3702..>4085)
                     /locus_tag="PLF85_13760"
     CDS             complement(3702..>4085)
                     /locus_tag="PLF85_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20783.1"
                     /translation="TAAKTSALATVANIKEAELQKFQNLLGDQQKLLDQSSGREKIDS
                     MKQDSEKRIQEEKDKATGKIEGLKGQTDSKIQEMKASTEGKVQQNTEKIDAMKGGTQQ
                     KIDSMKGSSVDEMKKKQEEMFKKFP"
BASE COUNT          819 a         1072 c         1063 g         1131 t
ORIGIN      
        1 gtcgcggctt tcgaccatca cgcacgcgcc gataatgtac gtaaagtcgt gcgtgtcgag
       61 aaactcggca actgccgcgt cagccgcgcc tggctgcagc cagacctttt tgtaaccgag
      121 ctccttcgcc ttgtgcaggc tttcgagcac cagcttcggt ggtatgacgt agaccagcag
      181 gtcgacgttg gtcttttcct tgagttcttc gaggctgtgc agcgcgggaa ctccctcaat
      241 cgacgtcgcc ttagggttga tgggatagac cgtgtaaccc ttggatttca ggtctttgaa
      301 gatgatgttg ccgtatttag tagagtcatt gctggcacca atcagcgcga cgggcggcat
      361 cgtgcggaag tatttaacca gcgtatgcat gcgacataat ccttgaggcg tatccggtgt
      421 caatcaacgg tgctgaacac ttgtgcccta tataaccgac cgaacaaggc ctcacttcac
      481 cttctcgaga attttctcaa cgtctacttt gtagcgttcg aagatttcat ttttcgccag
      541 gtcgaactgc agcagcgcaa tgttgtagtt cacgagcgac tcgagctcac gctggcggtt
      601 ggctaccatg ttttcaaggg cctggcctac aagctggaag ttgagcttac ctgtacgcgt
      661 gcgcgcgagc atgcgattgt agaaagcttc cgattctttg cgggaggttc tgctgttctg
      721 gaaaatttca aacgtcagac gtacattctc gagccgtgtc aggacttcgt cgcggatctc
      781 ctgctcgatg ttctgcagct tgatgcgcga ctgggtcagt tgcataccgg cgttacgctg
      841 gttaaccttg acctcttcgt cccagagcgg gtaagtcgct ttgactccca cctgccactg
      901 cgggctctgc agtgaacccg ccgatggaaa accggggaac atgatcatct ggtttgtacc
      961 ctgtgagacc aggttgaagt atgcgttgag cgagggtagc gcctggttac cgtagagatc
     1021 gctctgcatt tcagcgatct ccatttcgtt gcgtgcattc ttgaaatcga cgcgcttctc
     1081 gaacgcagct ttgagcgccg cgttggcgtc taggttgggc agggtatcga tgaggttggt
     1141 gataccttcg atcttggtat cgaccggcat attcacagtg cgcagcagct tacgcgttgc
     1201 attgcgcagg ctttgctcag tcatcgcaac cttggcaaca gagttggcta cacgcgcatt
     1261 gtagttgttg aggtcgtaac tttcgcccag gccgtagctc acgttacgcg cgacgatcgc
     1321 gcgcacctgg cggttcgatt tttcttcgag tttggcgttc tcgagcgcaa acttctgtat
     1381 ggtcacctgc cagtagtcgg tcagtgcctg caccacgaga cccgaaagca ggttcaccgt
     1441 gtacgcacgc tggctttcgg cctgcttttt cgcaatttta tccatcttgc ggtctgcata
     1501 accgaaagag ttcttgagga gttcctgttg aacattgatg aagtagccgt tgaaagtatt
     1561 cgtcgattgt ggcgtcggaa agttcattcc tgcaaacgag gtgattcccg cttcgttatg
     1621 cagctgctgg tagcggtaac caccaccgac agtagtaccg gtcacgaata gcttctggat
     1681 accgagtgct gcgttgtact ggtacccctt gctgttcgat gcaagcggtg catcactgaa
     1741 attcagatgc tggccttcag ctgatattat gggggcgtat ttcttctcta gcttgcgcgc
     1801 cgcggtatcg ctcatgatca catcatactt ggcagcctgc agtgttaggt tcttctcgag
     1861 tacaatatta atcgcgtcct gtatggtaag ctgaaccccg ccgatagtaa atgacgcctc
     1921 ggctggttgt gccgcggggg ctggtttatc agccgtttcg gttttagcag catctgccgg
     1981 gggggcttct tttttctcgg tgtcctgctg cgccgcaagc gcgagcgaaa gggacaggaa
     2041 aacaatccat gatttcttaa acatatggtt ctcatataaa aactaataga agaggtagaa
     2101 aacgcccaaa aaaattaacc aaatggttaa tagccccctc ccccgggctg cctcatgagc
     2161 gcacacggac ggaatgaatg ttaatctaca agacctttgg ggggttaaac taacccgtca
     2221 gaacgccgtt gcaagggcta ggtgctcagg cggcggcgtt gagtttgtga agatgacgta
     2281 tctttgcgtg tattcgagga caaatcttga attttcccgt gtttcacact ggtagttaat
     2341 aaaaatcccg gggcgcttgt ttggtcgccg ctcttggcgc gcctcccagt acgcgaaatt
     2401 ccgctgcgca cgcccgttgc gactataagg attcgtaagt actgcctcca gcaaccgcgg
     2461 catataatgc cgtatcgcaa aatcaagcat tcgtgaaatc gattcgcccg aatagatcgc
     2521 gcgctgccac aagattcgat acagcatttt gtctatatac caggccatac gacaatattt
     2581 gtgatccctc agttcatgat tatagcgccg tgcactcaca gtaaccatac cacgcccacg
     2641 ccaggcctgc aggtagagtt tggcaagtct gcgcagcagc tccgattctg accattcgat
     2701 acccctgctc ctgaccagtt tcaacgcggc tttgagcctt cgcgcactct tgtgacgcaa
     2761 cgagactgaa gtgtggcagt attgctgaga ggaaatatct ggcatcctac tatttaaata
     2821 ccagaaaatg gtgatttctt ctcatacttt ggggattttt tgcgccatat gctcctaagc
     2881 tcaaaatgca ctataccctt tggctgttta ctcctttcgc cctgtgccga aaatccttgc
     2941 cgtgctgacc tcgcgcagac ataaaaccca atggaccaaa atgtgaaatt gcgcaacctg
     3001 ctcttcggca gcgtaatcgc cctgcttctc tcctccattg ttttggcaat tgtacaccct
     3061 gctaagcccg tgaaggccat gcaacaaggg gtcaatatgc cggttgtcgg ccttgaaatg
     3121 gcatggacca ccgcagaggt ttgggatatt ctcggggacc cacagtccga tgccggtcaa
     3181 gaggcaaggc gcgccttcgc gctgggtacc tggcttgact ttggttacat cttttgttat
     3241 tctctggctt atcttttcct ggctctcttg ctggtacacc ggcattcggc tggccggata
     3301 tggatcgtgc tttcagcgct ggtcatttgt gtgaccgcat ttggtgacgt gatggagaac
     3361 agagcaatct ttagcattct cgacagcggt acacaatcgc tcgctgcaga acacgtcgat
     3421 gcactcgtcg tttttacccg tctgaaatgg ctgttcctcg gtattaacgg tctaccggca
     3481 gcggtccttt tacggcgcga aggcaaaagg ggattatctt ttattttgac tgcggcgttc
     3541 gcgtttggag ctttgggcgt tctgaaacag tacgcgattg agctgatgac tatcttcctg
     3601 gcgtttttct gggcctacct gctggtaaaa cttctgccgt tgaaaaaccg ttggtgggca
     3661 taatgcgcca gtggctgcct aaggcagcca ctggcggtag atcatggaaa ttttttaaac
     3721 atctcttctt gctttttctt catctcgtcg acactgctgc ctttcatcga gtcgatcttc
     3781 tgctgcgtgc cgcccttcat ggcgtcgatc ttctcggtgt tctgctgcac cttgccttcg
     3841 gtcgaagcct tcatttcctg gatcttgctg tccgtctgac ctttgaggcc ttcgatcttg
     3901 ccggtagctt tgtccttttc ttcctggatg cgtttttcgc tgtcctgctt catgctgtcg
     3961 attttctcgc gaccgctcga ctgatcgagc agcttctgct ggtcacccag caggttctgg
     4021 aatttctgca gctctgcttc tttgatattg gcaactgttg ccagcgcgct ggtcttcgca
     4081 gcagt
//
LOCUS       DAOQJF010000302         4044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13556_length_4044_cov_6.460266, whole genome shotgun sequence.
ACCESSION   DAOQJF010000302 DAOQJF010000000
VERSION     DAOQJF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13556_length_4044_cov_6.460266"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLF85_13765"
     CDS             <1..414
                     /locus_tag="PLF85_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20784.1"
                     /translation="DDFDAAPAKTPTQQFNAGLSAFAAQQFDEALAAYEDALSLGGAS
                     DPVLHEKLLYELGRTYVKKNNAKSAVEKFSELLRTFAHGTLARKTMIQLAEIYERAKD
                     TNRAAALYEKAAQMPPQDKEANIAHQKANQLRGRK"
     gene            411..1052
                     /locus_tag="PLF85_13770"
     CDS             411..1052
                     /locus_tag="PLF85_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE20785.1"
                     /translation="MTGGALFSKFGKQYNTGEILFCEFEPGNSFYLIQTGRVKISKVV
                     KDKEKTMDILGVGDIFGEMAILEEQPRSATATAIEPVTVLHFDRDNFVSMMMSQPQLA
                     FKLLVVFSKRIYDAKRRLMILLLDDIQSKIADVFLMLAEKEPTYASMREVSFPVTVDD
                     VANWAGVSADQVSEQLNHWAKIGKVELYSDRIFVRNLVDFKRIVAQKRKTEKR"
     gene            1094..1924
                     /locus_tag="PLF85_13775"
     CDS             1094..1924
                     /locus_tag="PLF85_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20786.1"
                     /translation="MLRKFTFALFFSASALLAQDSDKKSSSEQPATASQPAGGRIRDD
                     RVSVENVSFWKKNATDGKGDYLEVTFDIMNKSEDAIPLKMFVIAFNERDLVDKEYRRF
                     VEYPKWRVFDEDKKMHKLVLFDSIPATKHEDVAAFARKKEEAVAKNGGFKPREDADEN
                     TGSKKKTTLKQFLQYVQYVHENPNAGVDVMLQGYENTNYNIKCAGEAKAGKKCLEKKG
                     AYLIEEKALKVNVWAKLLARYTVDKKFFNHLGLILYDTESKKIVHRQFYSINGRFKIY
                     "
     gene            2029..2517
                     /locus_tag="PLF85_13780"
     CDS             2029..2517
                     /locus_tag="PLF85_13780"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNE20787.1"
                     /translation="MLTQRIGKIIERSREHWLFVPLFVLLILSFVYVFFIGDKGLMAY
                     RVRMREKEDLKTQIESLTRQKAELRQKLAMLRNKEMAVQKFSKDFYLFQEKVRILKFR
                     ESAEEKPAEQNESADLGTLQKSYIIISTLVIALVTLFFYRRNAAQMNLDRVRPFALSD
                     GE"
     gene            2514..>4044
                     /locus_tag="PLF85_13785"
     CDS             2514..>4044
                     /locus_tag="PLF85_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20788.1"
                     /translation="MIFRRLLPLFFAALLPLVAANKLEKYHAAVADYEARRYAQAVEL
                     FSQFVKENPYEPEVRKAWFYMTNALAEQGNCHEALARANITLDRYPVHPDKSELLVIS
                     AECLYRVNAHTRAEKTLSGLRRVEEMPSLAYRAERLRGMIAYDARGYARAEKHYRAAL
                     QHAARLGIEDTTLLRVNADLGRIYATDSLRTEEAVTYLSRAVALAEKFEPGQARSLKT
                     LLRRLSLRRIDKLNGLDDNSIADIRVDGDDVYVATWGGGLVRYTRSQDKLAKIPLPSP
                     QLRGLYADFEEIFVASYDGIFRISKKTGTTESINDAKGTLRLGQKIIKDDRFIYCSTL
                     SRGLIQYDSIKRGVITLGKDSWVKSDQVYAIDADLEYVAVGTLDQGALIHHKASGETI
                     QITKGENDLRSENVKAVLLDGRYVYIAAHNDGVYAYDLQQKKLRRLEAEIPFPSALAR
                     RENEIFIGTSGQGVRVLDRNTGRLERITAVEGLSSNEVQILRVEGEFLWIGYLEHGID
                     VL"
BASE COUNT         1029 a         1031 c         1099 g          885 t
ORIGIN      
        1 gatgatttcg acgcagcacc ggccaaaacc ccgacgcagc agtttaatgc cggcctgagc
       61 gcatttgccg cgcagcagtt tgacgaagct cttgctgcgt acgaagacgc tctctccctg
      121 gggggggcat cagatcccgt gttgcatgag aagctactct acgagctggg tcgcacctac
      181 gtgaagaaaa acaatgccaa gagcgcggtg gagaaattct ctgaattact gcggaccttc
      241 gcgcacggta cccttgcgcg caagaccatg atccagctgg cggaaattta cgagcgcgcc
      301 aaagatacta atcgtgcggc ggcgctgtat gagaaagccg cgcagatgcc accgcaggat
      361 aaagaggcaa acatcgcaca ccaaaaggca aaccagctca gggggcgcaa atgacgggcg
      421 gcgcgctgtt cagtaaattt ggcaagcaat acaatacggg tgaaattctt ttctgcgagt
      481 ttgagccggg caacagtttt tacctgatcc agactggtcg cgtgaagatc tccaaggttg
      541 ttaaagataa agaaaagacg atggatattc tcggggtagg cgacattttc ggtgagatgg
      601 ccattctcga agaacagccg cgatcagcca cggcgacagc cattgaaccc gttacagtcc
      661 tgcattttga ccgggacaac tttgtctcga tgatgatgag ccagccgcag ctggctttta
      721 agctactggt ggtattctcc aagcgtattt atgacgccaa gcggcgcctc atgatactgc
      781 tgctcgacga catccaatcg aaaatagccg acgtttttct gatgctcgca gagaaagagc
      841 cgacgtatgc cagcatgcgc gaagtgtctt ttcccgtaac tgtagacgat gtcgccaact
      901 gggcaggggt gagtgccgac caggtttcag agcagctcaa ccactgggct aaaatcggca
      961 aggtggagct ttacagcgac cgtatttttg tccgcaacct tgtggatttc aagcgaatcg
     1021 tagcacagaa gcgtaagaca gaaaaaagat aaataacaga tggaaaattg ccgacattca
     1081 aatgaggtat atcatgttac gtaaattcac tttcgccctc ttcttctcgg cttcggcgct
     1141 gttagcgcag gattcagaca aaaagtcatc ttcagagcag ccggctactg cctcacaacc
     1201 ggcaggcggg cgcatacgtg acgaccgcgt ttcggttgaa aacgtcagtt tctggaagaa
     1261 gaacgctacc gacgggaaag gtgactatct cgaggtaacg ttcgatatca tgaataagag
     1321 cgaagacgct attccgctca agatgttcgt catcgccttt aacgagcgtg atctggtgga
     1381 caaagaatac cgccgtttcg tcgaataccc aaaatggcgc gttttcgacg aagacaagaa
     1441 aatgcacaag ctggtgctct ttgacagtat tccggcgacg aaacatgaag acgtggcggc
     1501 cttcgcccgc aaaaaagaag aagctgttgc caagaacggc gggttcaagc cccgggaaga
     1561 tgcagacgag aataccgggt caaagaagaa gaccacgctg aagcagtttc tgcagtacgt
     1621 gcagtatgtt cacgaaaacc caaacgccgg tgtggacgtt atgctacagg gttacgaaaa
     1681 cacgaactat aacatcaagt gcgctggtga ggcgaaagcc ggcaaaaaat gcctcgagaa
     1741 gaagggcgcc tacctgatcg aagaaaaagc gctcaaagtg aatgtctggg ccaagttgct
     1801 cgcgcgttac acggtagata agaaattctt caatcacctc ggccttattt tgtacgatac
     1861 tgagtcgaaa aagatcgtcc atcgccagtt ctacagtatc aacggccgtt tcaaaatcta
     1921 ctagattcca gaccgggctc ctgaacaagg agcctgagcc ttgtggcaca taacggccgc
     1981 ccaatgcata ttgattgtcg ccgcagttga tcctctttac ctgacgccgt gctgacgcag
     2041 cgtattggca aaattatcga gcgcagccgt gaacactggc tgtttgtgcc gctttttgtt
     2101 ctcctgatcc tctcttttgt ctacgtgttt ttcattggtg acaagggtct gatggcttac
     2161 cgggttcgta tgcgtgaaaa agaagatctc aagacgcaga ttgaaagcct tacacgccaa
     2221 aaggcggagc tgaggcaaaa gcttgccatg ctccggaaca aagagatggc agtgcagaag
     2281 ttttccaagg atttttacct tttccaagag aaggtgcgga tcctgaaatt tcgggagtcc
     2341 gctgaagaga agccggcgga acagaatgaa tcggccgacc tcggtaccct gcagaagtct
     2401 tatatcatca tttcgaccct ggtgatcgcc ctcgtaacgc tcttctttta ccgccgcaat
     2461 gcggcacaga tgaatcttga ccgtgtgcga ccgtttgctt tgagtgacgg cgaatgatct
     2521 ttcgtcgttt gttgccactg tttttcgcag cgttactgcc gcttgttgcg gccaacaagc
     2581 tggagaaata ccacgctgca gtagccgatt acgaagcccg gcgttacgca caggcagtgg
     2641 agctattttc gcaattcgtc aaagagaacc cgtatgaacc cgaagttcgc aaggcatggt
     2701 tctacatgac aaatgcgctc gccgagcagg gcaactgcca cgaggcgctg gcaagagcga
     2761 acatcacact cgaccgctac ccggtacatc ccgataagtc tgaactgctc gtcatcagtg
     2821 ccgagtgttt ataccgtgtg aatgcgcaca cccgcgctga gaagacactt tcgggcctgc
     2881 gcagggttga agagatgccg tcgctcgcct accgggcaga acgcctgcgc ggaatgatcg
     2941 cttacgatgc gcgggggtat gcccgcgccg aaaaacacta ccgggcggct cttcaacacg
     3001 ccgccaggct cggtattgaa gatacaacgc tgctcagggt gaatgccgat cttggccgca
     3061 tatatgccac cgattcactt cggactgaag aagccgtaac ttacctgtcg cgcgcggttg
     3121 cgctggcaga aaagtttgaa cctggccagg cgcggtcgct gaaaacactg ctgcggcggt
     3181 tgtctctgcg gcgtatcgac aagctgaacg gtctcgacga taattcgatt gccgatatcc
     3241 gggttgacgg cgacgacgtc tatgttgcca cctggggcgg tggactggtc cgttacacac
     3301 gttcgcagga taagctggca aaaattccac tgccttcacc gcagctgcgg ggactctatg
     3361 ccgatttcga agagatcttt gtcgcttcgt acgatggtat ttttcgtatc agcaaaaaga
     3421 ccggcacgac cgagtccatt aatgacgcta aaggaacgct ccgccttggt cagaaaatca
     3481 tcaaagatga ccgcttcatc tactgttcga cactcagccg cggcctgata cagtatgaca
     3541 gtatcaagag gggcgtcatc acgctcggta aagattcgtg ggtaaaatct gaccaggtat
     3601 acgcgatcga tgcagacctc gagtatgtcg ccgttggtac cctcgaccag ggggcgctga
     3661 ttcaccacaa ggcgagcggc gaaaccatac agattacgaa gggcgagaat gacctgcgtt
     3721 cagagaatgt taaagcggtt ttactggacg gtcgttacgt ctatatcgcc gcacacaatg
     3781 acggtgtgta cgcgtatgac ctgcagcaaa agaagctccg tcgcttagaa gccgagatac
     3841 cttttccgtc ggcgctcgcg cgccgtgaaa acgaaatatt tatcggcact tcagggcagg
     3901 gggtgcgcgt gctcgatcgt aacaccggca gactcgagcg tattaccgca gtcgaagggc
     3961 tgtcatctaa cgaagtgcag atactgcgcg ttgaagggga gtttctctgg attggttacc
     4021 ttgagcacgg tatcgacgtt ctct
//
LOCUS       DAOQJF010000303         4041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13567_length_4041_cov_5.542148, whole genome shotgun sequence.
ACCESSION   DAOQJF010000303 DAOQJF010000000
VERSION     DAOQJF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13567_length_4041_cov_5.542148"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLF85_13790"
     CDS             complement(<1..896)
                     /locus_tag="PLF85_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE20789.1"
                     /translation="MAPNRHLHKMHDAFVVPEGSEFSDEFYERNLPPEAGEIKKILAK
                     INEHFHEDYAVSFTKHVAEFIDRMYFRSRWVGFDELPKRNNPPLPIIYISNHSGMAFP
                     WDAIIFSSGLLKRAGYQLKDAARPLIYKALAQTDYLNPYMMHNFWRKVGCIDATLDNF
                     EAVMHSSLADVLIYPEGVGGISKGFDKRYKLQHFSLSFTRMALKFKVDIVPVATINGE
                     YINPYSYRIEQINKAIQKVIKLPFLPVGPMLGLLPASPWAFYFGMPAKLTYVMGEPIK
                     IYEMTDKDPAKITKGELRHITN"
     gene            1032..2045
                     /locus_tag="PLF85_13795"
     CDS             1032..2045
                     /locus_tag="PLF85_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20790.1"
                     /translation="MQRNQIMSLTMAFTAAVLSGTQPLIAQSGPAVVTTAPSGTVVVS
                     DNSNRQVNLKDNLQDRITLIDGTSLVGKITEMNASRVIIKVAGQDLVVEPNKIERVER
                     NILFDPDAEKRRAVEVKTKDGSRYRGSISRADATTTYVQTANGEVPVRNDNVEKIDYL
                     DVEKVRQADAIAARPKKWELTLKGGSMLYQLGTFKDLLSPGYFGLLQVEAPHFLLPWN
                     LRLSPGLQAGYVRNSGKSVSTTKIDLFPGVLTVALSRQLGTLPLDIFVQGNVGVNLTR
                     GIQANANEKLSLDFAYGAELGAKYHLNEMINFRLAGIWHAVSESTATLNHLGAYASVG
                     LMF"
     gene            2069..3283
                     /locus_tag="PLF85_13800"
     CDS             2069..3283
                     /locus_tag="PLF85_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNE20791.1"
                     /translation="MKRFIPMLLGLTLAAANCTTNSTLLSDTAASLGGASSASGSGTS
                     TTTSGSKLWVVGGTSGALSAALSTVLSYSPDTNAWTTETTSGTYTPVSFAAYARYGTK
                     IYVFGGFNAAGTAVSTTQIFDTSTLAWSTGGALPAAMAHGSAVVVGTKILVLGGTSVN
                     AATAWAVSTMNALYDPATDTATAQTALGGTTGQGSDKCAVADGTAVIHNTARTAAATI
                     TAATQAFFLNAGLATATPTTTTLGTTLTNRGGMACVLYSPGSAGNSNFILAIGGLTAN
                     TANTGGFVGGANNTAGGAGTLTAWTSVTTVQNLAAPYTGAWGTSLALPTATSFAGAAI
                     LNNVLYVVGGNTGTAAAQNIQATTNVYAASLSANGTSAPFLGWSANKPGTSTALQALP
                     VARWGHGLIAIP"
     gene            3280..3711
                     /locus_tag="PLF85_13805"
     CDS             3280..3711
                     /locus_tag="PLF85_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20792.1"
                     /translation="MKRSHELLATAVALTAMVLLTACAGKSLRPGESAPLTDKAGKAY
                     GTRVQVAENVERHRIDADQDGSVEKQYDFNRGVITYSEHYYPDGKVRVRTSYLNGKAN
                     RSEVFNPDGTLRGIAYHSPQELVDTVDLPSRNRRVEFLPQK"
     gene            3749..>4041
                     /locus_tag="PLF85_13810"
     CDS             3749..>4041
                     /locus_tag="PLF85_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glucose cotransporter"
                     /protein_id="HNE20793.1"
                     /translation="MFSPVDYFILFSYFVLILGVGLWVSRPKPGEDKNAADYFLAGKG
                     LSWWVIGASLIASNISAEQFIGMSGSGFAIGMGIASYEFMAAITLIIVAVFFMP"
BASE COUNT          922 a         1137 c         1117 g          865 t
ORIGIN      
        1 tggtttgtga tgtgcctgag ctcccccttg gtaatttttg cagggtcttt gtcagtcatc
       61 tcgtagatct taatcggctc acccatcacg taggtgagtt tggccggcat gccaaagtaa
      121 aaggcccacg ggctggcagg cagaagtccg agcataggcc ccacaggcag gaaaggcagc
      181 ttgataactt tttggattgc cttgttgatc tgctcgatgc ggtagctgta gggattgata
      241 tactcaccgt taatagtcgc aacaggaaca atatcgacct tgaacttgag cgccatccgc
      301 gtgaacgaaa gcgaaaaatg ctgcagcttg tagcgcttgt cgaagccctt gctgattccg
      361 ccgacgccct cggggtatat gagcacgtcg gccagcgagg aatgcataac tgcttcaaag
      421 ttatcgaggg tcgcatcgat gcagcccacc ttgcgccaga aattatgcat catgtagggg
      481 ttgaggtaat cggtctgcgc gagcgccttg tagatgaggg ggcgcgcggc atctttcagc
      541 tggtaaccgg cccgtttcag caagccgctc gagaagatga tcgcgtccca cggaaaggcc
      601 atgcccgagt ggttgctgat gtagatgatc ggcagcggcg gattattgcg tttgggcaat
      661 tcgtcaaaac ccacccaacg cgagcgaaag tacatacgat cgataaactc cgccacgtgc
      721 ttcgtgaaac tgacggcata gtcttcgtga aagtgctcgt tgatttttgc aagtatcttc
      781 ttgatctcgc ctgcttcggg tggcaggttg cgttcataga attcatcgct gaattcgctt
      841 ccctcgggta cgacaaatgc gtcgtgcatt ttgtgcaggt gcctgttcgg tgccatgtat
      901 ctttcctgcc accacggtac gtggcaggcc acaagtgtaa aacctttacc ggcgcctacc
      961 atcaagttac cgccagccgg ctgtcgagaa tcgagtagta atatcgtcta tttaggatgt
     1021 aaggggaaca catgcaaaga aatcagatta tgtcgcttac catggccttc acggccgcag
     1081 tcctgagcgg aacccagccg cttatcgccc agtcgggtcc ggcggtagtg acaactgccc
     1141 ccagcggcac ggtcgttgtt tctgacaaca gcaaccgcca ggttaacctg aaggataacc
     1201 tccaggatcg catcacactc atcgatggaa cgtcgctggt gggcaagatt accgagatga
     1261 atgcaagccg tgttatcatt aaggttgccg gccaggattt ggtcgttgaa ccgaacaaaa
     1321 tcgagcgcgt tgagcgcaac atactcttcg acccggacgc ggaaaaacgc cgggcagtcg
     1381 aagttaagac caaagatggt tcacgatatc gtggtagcat ctctcgtgca gacgcaacga
     1441 caacatatgt acagactgca aatggagaag taccggtcag gaacgacaat gtcgaaaaga
     1501 tcgattatct ggatgttgaa aaagtcaggc aagctgatgc gatagcggcg cgtccgaaaa
     1561 aatgggagct aacgctgaag ggtggttcaa tgctctacca actgggcacg ttcaaagacc
     1621 tgctttctcc gggctatttt ggcctgttgc aggtagaagc tccgcacttc ttgctcccct
     1681 ggaatcttcg cctcagcccg ggcctgcagg ccggttatgt gcgcaactct ggcaaatcgg
     1741 tcagcaccac aaagatcgat ctttttccgg gagtgctgac cgtggcgctg agccgccaac
     1801 tgggcacgct gccgctcgat attttcgtgc agggtaacgt gggagtaaac ctcacgcgcg
     1861 gcattcaggc aaacgcgaac gaaaaactgt cgctcgactt tgcctacggt gcagaactgg
     1921 gcgcgaaata ccacctgaac gaaatgatta actttcgcct cgcaggtatc tggcacgctg
     1981 tgtcagaaag tacagccacg ctcaaccacc tcggagcata cgcctcggtc ggcctgatgt
     2041 tctgaaagaa actcaaaagg agattatcat gaaacgattc atacccatgc tgctcggact
     2101 cacgctcgcg gcagccaact gcactaccaa ttcaaccctg ctcagcgaca ccgctgcgag
     2161 tctgggcgga gcttcctctg cttctggcag cggcacgagt actacaactt ctggcagcaa
     2221 actctgggtt gtcggtggca cctcgggtgc actgtcagct gccctgagca cggtgctgtc
     2281 ttactcacca gacacaaacg catggactac ggaaaccact tcgggcactt acaccccggt
     2341 aagtttcgca gcctatgccc gctatggcac aaagatttat gttttcggag gctttaacgc
     2401 cgcaggaact gccgtcagca cgacacaaat ctttgataca agcacccttg cctggagcac
     2461 cggcggtgca ttgcccgctg caatggcgca cggtagtgct gtagtagtag gaacgaaaat
     2521 actcgtgctg ggtggcacca gtgtcaacgc ggccacagcg tgggctgtat cgacgatgaa
     2581 tgcgttgtat gatccggcta ctgacacggc gacggcacag acagcactgg gcggcacgac
     2641 tggccagggc agcgacaaat gcgccgttgc cgatggtacg gcggtaatcc acaacaccgc
     2701 acggactgcc gccgcgacga ttaccgctgc cacacaggca ttctttctca atgccggcct
     2761 ggcgaccgca acacctacga caacgacact cgggaccacg ctcacgaacc gcggtggcat
     2821 ggcgtgcgtt ctttattcgc ccggctcagc aggaaacagc aacttcatac tcgctattgg
     2881 tggcttaacc gccaacactg ccaacacggg gggctttgtc ggtggcgcga ataacacagc
     2941 cggcggcgcg ggaactctga ccgcgtggac ttcagtcacg accgttcaga acctcgcagc
     3001 accctacact ggcgcatggg gcacaagcct ggcgctgccg acagctacgt cctttgctgg
     3061 tgcggcgatt ctaaacaacg tgctctacgt tgttggggga aatactggta ccgcagcggc
     3121 tcagaacatc caggcgacga caaacgtcta tgctgcatca ctgagtgcga acgggacgtc
     3181 tgcgccattc ctgggctgga gcgcaaacaa accgggaacc agcaccgccc tgcaggctct
     3241 gcctgtagca cgctggggcc acggcctgat cgccattcca tgaaacgcag tcacgaactc
     3301 ctcgccactg ccgtcgcact gacggcaatg gtactgctga ctgcctgcgc cggcaaatcg
     3361 ctcaggccgg gcgagagcgc gccgctcact gacaaagcag gcaaggcata cggtacacgt
     3421 gtccaggttg cagaaaacgt tgagcggcac cgtatcgacg ctgaccagga cggcagcgtg
     3481 gaaaaacagt atgactttaa ccgcggggtc atcacttaca gcgagcatta ctatccggat
     3541 ggcaaggtca gggtgcgcac ttcttacctg aacggcaagg ccaaccggtc agaggtgttt
     3601 aatcccgacg gaactctgcg cggtatcgcc taccacagtc cgcaggagct cgtcgatacc
     3661 gtcgatctgc cgtcgcgcaa ccgccgcgtc gagtttctac cacagaagta gttgtgcctg
     3721 agtatcggca gctaaacatg agtgcctcat gtttagcccg gtcgactact ttatcctgtt
     3781 ttcttatttt gtcttgatac tcggcgttgg cctctgggta tcgcgcccaa agccaggcga
     3841 agacaaaaac gctgccgact actttctcgc cggcaagggc ctcagctggt gggtgattgg
     3901 cgcatcgctg atcgcctcga atatttcagc cgaacaattc atcggcatgt cgggttcggg
     3961 ttttgcgatc ggcatgggta tcgcgtctta tgaattcatg gctgcgatca ccctcattat
     4021 cgtcgcggtc ttcttcatgc c
//
LOCUS       DAOQJF010000304         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13635_length_4027_cov_4.803877, whole genome shotgun sequence.
ACCESSION   DAOQJF010000304 DAOQJF010000000
VERSION     DAOQJF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13635_length_4027_cov_4.803877"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1913
                     /locus_tag="PLF85_13815"
     CDS             <1..1913
                     /locus_tag="PLF85_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE20794.1"
                     /translation="FTGLSREQLLLILLLTGITAGLLQLLLALFKGGRLIKFVPYPVI
                     AGYLSAVAVIIFFGQIARAFPELHIARWSDFSWQIFHWVNLPVLVVTVTLVFLAAKIS
                     KRVPAPIIALSGGMVTFALIGLTDGSLFDTANSPYLVGRIRTGGQIINLPPWQLIASI
                     SPAIWPKIFASGAMLAMILSVDTLKTMVILDNLKHVRADSDKVLRGLGVANTVSSLVG
                     GMPGSGSANSSLINMNSGASHWSSVVFSGVFALIILLFLSDYAAYIPIAALAGLLVAS
                     SIRLFDTSIFQLLKKRSTRLDFAVAMLVIITGITYNLIVAAGAGIALSIFLFVRNQIR
                     DTVIRSKTRGDRKFSNKSRMPDEMQVLRDHGHETIIYSLGGNLFFGTTDQLYRDIEAE
                     LKTSRNVVLSFKEVHSIDFTAVHMLELLDSVVHKQGGELLLCSLVGELTRGVDFEGYL
                     SHAAVAHKEPHWRIFPDANTSLMYLEEKILSEHGQGIHKKLTFALSEMDVFTGCDAAA
                     LTELSSIVTTEKYEASRPVFLQGSHSEELYFIGHGVVDIVLPAHDGPARRLSTFAQGD
                     FFGDMAFLDSEERSADAIAAAGTLLYSLKRADFEAFCTHHHKFGEVFYRNLARILAHR
                     LRQSHHELQAAL"
     gene            1952..2596
                     /locus_tag="PLF85_13820"
     CDS             1952..2596
                     /locus_tag="PLF85_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNE20795.1"
                     /translation="MAAVLFCGAILASGLLIGRAVGAYNGYIVKSPHKVPAVYTGIVP
                     GARVYSDGRLSHMLEDRVVSAAQLYNQGKIRKILLTGDHGNNEYDEVKAMRLHLRRLG
                     VPESAIFLDHAGFTTYDSLYRARDIFGVKDCVIVTQEFHLPRSVYTARRLGLDCYGYV
                     ADRRPYTARSHGWSKFREFFSKIKAWLQVNVTQPKPRYLGEKIPITGDAALTRD"
     gene            complement(2598..3041)
                     /locus_tag="PLF85_13825"
     CDS             complement(2598..3041)
                     /locus_tag="PLF85_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE20796.1"
                     /translation="MDIRELSADDLAAWLPLWQAYQRFYNTDISEKTTRTTWSRLTDP
                     AEPMFALGAFSKRELVGFAHIIYHRSCWTEGNYCYLQDLFTPEEMRGKGIGTALINAV
                     YERARADGASRVHWLTHESNLAAQSLYEKVAVRSGFVQYRKVLSL"
     gene            3117..3311
                     /locus_tag="PLF85_13830"
     CDS             3117..3311
                     /locus_tag="PLF85_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20797.1"
                     /translation="MLANEHPHIFSQPRKHPFVTGEIVHRHCALVVGECRLRALRVVD
                     FGDAAEAWLEPGRKLVVRGA"
     gene            complement(3315..>4027)
                     /locus_tag="PLF85_13835"
     CDS             complement(3315..>4027)
                     /locus_tag="PLF85_13835"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE20798.1"
                     /translation="VACSTQCLMYHIQLRQALPKLFLMLLIANGGAILAQSKKVFVAT
                     SAGLNLREAPETTAKKLLTIPYGAQIQIEPTDQKITLEGIEGSWIKTSFKGKTGYAAD
                     VYLVSLPLPAARVSSLWNYLAQIAPGKDIGGNDAVRLATEFGITQSRGEGAGCIDGSR
                     IIFSKNSRAEVLALTRIIYLKRFPALKKSYADFTGKAASAAARNSNGEKGIQQIQVYE
                     DAGKVHFTDTFEYKPCTP"
BASE COUNT          814 a         1118 c         1134 g          961 t
ORIGIN      
        1 agttcacggg actttcgcgc gaacaactgc tgctcattct tttgctgacg ggcatcaccg
       61 caggtctgct gcagctgctg ctcgcgctct tcaagggggg ccggctcatc aagtttgtgc
      121 catacccggt gatcgcaggc tatctcagtg ccgtcgcggt tatcatcttc ttcgggcaga
      181 tcgcgcgcgc ttttcccgaa ctgcatattg cgcgatggtc cgatttttcc tggcagattt
      241 ttcactgggt gaatctgccg gtgctggttg tcaccgtcac cttggttttc ctggcggcaa
      301 aaatatcgaa gcgtgtgccg gcgccgatca ttgcgctgag cggaggtatg gtgacgtttg
      361 ctctgattgg cctcaccgac ggttcactgt tcgatacggc aaacagcccc tacctggttg
      421 gccgcatcag aacaggcggg cagattatca acctgccgcc atggcagtta atcgcctcta
      481 tctcccccgc aatctggcca aaaatattcg cctcgggtgc gatgctggcg atgattctct
      541 cggtcgatac gctcaagacc atggtgattc tcgataacct gaagcacgtg cgcgccgaca
      601 gcgataaggt gctcaggggg ctcggtgtgg ccaacactgt ttcatcatta gtgggaggta
      661 tgcctggttc tggctctgcc aactcttcgc tcatcaacat gaactcgggt gccagccatt
      721 ggtcgtcggt ggttttttcg ggtgttttcg cgctaatcat tcttctcttt ctctcagact
      781 atgccgcata cattccgatc gctgcacttg caggcctgct ggtcgcgagc tcgattcgcc
      841 tgtttgacac gagtatcttt caactgctca agaagcgcag cacgcggctc gattttgccg
      901 tggcgatgct tgtgattatt acgggcatca cgtataatct cattgtcgcg gcaggtgccg
      961 gtatcgcgct ttcgattttt ctcttcgtgc gtaaccagat tcgcgatacg gtgattcgca
     1021 gcaaaacccg cggcgaccgc aagttctcga acaagagccg catgcctgat gagatgcagg
     1081 tgctgcgcga tcacgggcac gagaccatca tctattcact cgggggtaat ctttttttcg
     1141 gtaccaccga ccagctctac cgggacatcg aagcagaact taagaccagc cgcaacgtgg
     1201 tactgtcgtt caaagaggtg cactcgatcg actttaccgc agtgcatatg ctcgaattac
     1261 tcgacagcgt tgtgcacaaa cagggcggcg aattgctgct ctgttctctt gtcggtgagc
     1321 tgacgcgcgg tgtggatttc gaaggttacc tgagccacgc ggccgtggcg cataaagagc
     1381 cgcactggcg tatttttccc gatgcaaata cctcgctgat gtacctcgaa gagaaaatac
     1441 tcagcgagca cggccagggt attcacaaaa agctcacctt tgccctcagt gaaatggatg
     1501 tattcacggg gtgcgatgcg gccgcgctca ctgagctctc atcgatcgtt accacagaaa
     1561 aatacgaggc ttcgcgcccg gtattcttgc agggcagtca cagcgaagag ctgtacttta
     1621 ttggccatgg agtggtagat attgtgctgc ccgcacacga cggccctgcg cgcaggctca
     1681 gcacctttgc gcaaggtgat tttttcggcg acatggcctt tctcgacagc gaagagcgtt
     1741 cggccgacgc tatcgcggct gcgggtacgt tactctattc gctgaaacgg gccgattttg
     1801 aagccttctg tacacaccac cacaagttcg gcgaagtttt ctaccgcaac ctggcgcgta
     1861 tcctggcgca ccggctgcgc cagtcacacc acgaactgca ggcggcgctg tgaagatctg
     1921 gaggcggtat ttcagcctca gaaaccttct tatggccgca gtgctgttct gcggtgcaat
     1981 tctcgccagt gggttactca tcggccgcgc cgtcggtgct tacaacgggt acattgtgaa
     2041 atccccacac aaagtgccgg cggtttacac cgggattgta ccgggcgccc gcgtctacag
     2101 cgatggcagg ttgtcgcaca tgctcgaaga ccgtgtcgtt tctgcggcac aactctacaa
     2161 ccaaggcaag attcgtaaga ttctgctgac gggagatcac ggcaataacg agtacgacga
     2221 ggtcaaagca atgcgcctgc acctgcgccg cctcggtgta cccgaaagcg ctatctttct
     2281 cgaccatgcc gggtttacca cgtacgacag cctgtaccgg gcgcgcgata tttttggcgt
     2341 gaaggattgt gtgatcgtca ctcaggaatt tcacctgccg cggtcggtat acaccgcgcg
     2401 cagactcggg ctggactgtt acggctacgt ggccgatcgc cgcccgtaca ccgcccgttc
     2461 tcacggctgg tcaaagtttc gtgagttttt ctcgaagatc aaggcatggc tgcaggtgaa
     2521 tgtcacgcag ccaaaaccca ggtacctcgg tgagaaaata ccgattacgg gtgatgctgc
     2581 gctgacgaga gattgaacta gagactcaaa actttgcggt actgcacgaa gcccgaccgc
     2641 acggcaacct tctcataaag gctctgcgca gccaggttgc tttcgtgtgt cagccagtgt
     2701 acacgcgaag ctccgtctgc tctggccctt tcatagacgg cattgatcag cgccgtacca
     2761 attcccttgc cgcgcatttc ttcgggggta aagagatcct gcaggtagca gtagttgcct
     2821 tcagtccagc acgagcggtg gtagatgatg tgcgcgaagc cgacgagctc ccgtttgctg
     2881 aaagcgccca gagcaaacat gggttcggcg gggtcggtga ggcgtgacca cgtggtacgg
     2941 gtcgtttttt ctgagatgtc ggtattgtag aagcgctggt aggcctgcca aagcggcagc
     3001 caggcagcca gatcgtcagc cgacagttcc ctgatatcca taagtcgtaa cttttcggtg
     3061 caaagcgagg gtcaagggga aaggcagcta tcgcaaagct gcaattatgc gcctttttgc
     3121 ttgcaaacga gcacccgcat atcttcagcc agccgcgcaa acacccgttt gtcaccggtg
     3181 agattgtaca tcgtcattgt gccctcgtag taggtgaatg ccgtctgcgc gcgctgcgcg
     3241 tcgtcgattt cggcgatgca gccgaagcct ggcttgagcc ggggcgcaag ctggtcgtac
     3301 ggggggccta acgatcaggg cgtacagggc ttgtactcaa acgtgtcggt gaaatgcacc
     3361 ttgcctgcgt cctcgtaaac ttggatctgc tgaattccct tttcgccgtt agaattgcgc
     3421 gcagcggcgc tagcagcctt acccgtgaaa tctgcgtacg attttttcag ggctggaaag
     3481 cgcttcaggt agattatgcg cgtgagcgcc aagacttccg cgcgggagtt cttgctgaaa
     3541 ataatgcggc taccgtcaat gcagcctgca ccctcgccgc ggctctgcgt gatgccaaat
     3601 tcagttgcca gtcgcacagc atcgttgccg ccaatgtctt taccgggagc gatctgcgcc
     3661 aggtaattcc agaggcttga gaccctggcg gcaggcagtg gcaatgacac caggtagaca
     3721 tcggcagcat aacctgtctt acctttgaaa gaggtcttga tccacgaacc ctcaattcct
     3781 tcgagcgtaa tcttttggtc tgtaggttcg atttgaatct gcgcgccgta gggtatagtg
     3841 agcaactttt ttgccgtggt ctccggcgcc tcgcgcaggt tgaggcccgc gctcgtcgca
     3901 acaaatacct tttttgactg ggcgagtatc gcacccccgt ttgcgatgag caacatcagg
     3961 aataattttg gcagtgcctg ccgaagctgt atgtggtaca tgaggcactg cgttgagcag
     4021 gcgacat
//
LOCUS       DAOQJF010000305         4002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13754_length_4002_cov_4.467950, whole genome shotgun sequence.
ACCESSION   DAOQJF010000305 DAOQJF010000000
VERSION     DAOQJF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13754_length_4002_cov_4.467950"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PLF85_13840"
     CDS             <1..1191
                     /locus_tag="PLF85_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE20799.1"
                     /translation="KDKIVAEVCAGVDFLMKKNKVERFLGVGSLVSASEVQVLDPAGG
                     STALTDRKVIETIKAKKIIIATGSVPIELPTVPVDGKAIITSDHAIALTRVPKHMVVI
                     GAGVIGLELGSVWRRLGSKVTVVEALPRLFGTADRQGSALAQRILEQQGIEFLFEHKV
                     EKAEAKKGASTALGNHAVVTVKAPTGESKAIEADVVLCAIGRKPYLEGLGLDKVGVKL
                     TERGRIDVNFHNWQTSVPGVYAIGDCINGPMLAHKAMEEGVAVVEVMAGQAGHVNYDV
                     IPGVVYTHPEVAWVGLGEEELKEKGIEYNVGRSFFKPNGRAKAMMEGDGQLKILADKK
                     TDRILGAFCVGAQASQLIAELTLAMEFGASSEDVARTCHAHPTLSEIVKDAAESAGGW
                     AIHQ"
     gene            1254..1853
                     /locus_tag="PLF85_13845"
     CDS             1254..1853
                     /locus_tag="PLF85_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNE20800.1"
                     /translation="MKIYGMSSSGNCHKIRLIVNYLKIPHEWIETDPRHGATKTAEFR
                     QMNPNAHVPVVQLDDGTYLPESNAILYYLAQGSSFWPIDKLSQARVLQWMFFEQYDHE
                     PRIAVNRSIQSVTNTLAERRAEYEANLPKGYRALGVMESHLAEQRWFGADVASIADIA
                     LYAYTHVADEGGFSLADFPHIRAWLGRMESLEGHSRMKL"
     gene            complement(1882..2634)
                     /locus_tag="PLF85_13850"
     CDS             complement(1882..2634)
                     /locus_tag="PLF85_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20801.1"
                     /translation="MVVYPFVRVTGQVESSHPGPEHSASETEILMRQSPVGERIRQRT
                     LSANVKAAERFFTRHLPAGWKFTTSERQVLLRRVAPVYALAVEPQDYLTSSKSTLLKR
                     AKTSGKRKDCIMEFKVERHDDDALMRQKLRLYKEIRRDTARAFDRLALKRLCGTVTPE
                     ACAKLPGQAGTAAQEFMSIRAILEDKLEAVPLYRIGTLYLFPLKHQCVTAKLDWYTIN
                     SEYPAGESIFPLEAREEIEVILRNLEQLKLSE"
     gene            2633..3451
                     /locus_tag="PLF85_13855"
     CDS             2633..3451
                     /locus_tag="PLF85_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20802.1"
                     /translation="MQQTIAHRMIALLAMAVFPFALAAQDSVKPKKAAEKPAAKKEET
                     PPAKEGEKSGAREVTYLDLVHARDKALKLKEDNMRDIQLLNALGAAMPEAVDKGASDQ
                     IATEYKDALKLIYRMEYVGAEKTLRTNRENIAAAMGKASEVFRKKTTEMLNQSADRMA
                     DLDMQAENTGSAESIRMAGVVQRNQHRLSVGYQQLTLASTAEKHRRYAEALSHYRLAR
                     LHAIYLQIELAKDENEKNSLRAKYRNELDEKRIVKEESAEKAAPPAPEKKPAGK"
     gene            3566..3841
                     /locus_tag="PLF85_13860"
     CDS             3566..3841
                     /locus_tag="PLF85_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20803.1"
                     /translation="MGQQQAILFATGVLVSAFSALLAGWLVKTRNGAGWVYYGAITLR
                     MLALLAGCTLVYLLASPGAGSLIGAYALGVAAAWLVHVAVALVQLRE"
BASE COUNT          885 a         1115 c         1179 g          823 t
ORIGIN      
        1 aaggacaaga tcgtcgccga ggtctgcgcc ggcgtcgact tcttaatgaa gaagaacaag
       61 gtggagcgct ttctaggcgt tggctctctc gtctctgcaa gcgaagtgca ggtgttggat
      121 cctgcgggcg gttcgactgc gctcaccgac cgcaaggtta tagagaccat caaggcgaag
      181 aagatcatca tcgccacggg ttcggtaccg attgaactgc cgaccgtgcc ggttgacggc
      241 aaggcaatca tcacctctga ccacgcgatc gcgctcacca gggtgcccaa gcatatggtc
      301 gtgatcggtg cgggcgttat cgggttggag ctcggcagtg tatggcgcag gctcggcagc
      361 aaggtgactg tcgtcgaagc actgccacgg ctcttcggca ccgctgaccg gcaggggtct
      421 gccctcgcgc agcgcattct cgagcagcag ggcatcgagt tcttgtttga gcataaagtc
      481 gaaaaagcgg aagcgaaaaa gggtgcctcg actgcgctcg gcaaccatgc cgtggtcaca
      541 gtgaaagctc ccacaggcga aagcaaggcg atcgaagctg atgtcgtgct gtgcgcgatc
      601 ggccgcaagc cgtacctcga aggtctaggc ctcgacaagg tcggcgtcaa gctcaccgag
      661 cgcggccgca tcgatgtgaa tttccataac tggcagacat cggtgccggg cgtctacgcg
      721 atcggcgact gcatcaacgg cccgatgctc gcgcacaagg cgatggaaga gggcgtcgca
      781 gtcgttgaag tcatggccgg ccaggcaggc cacgtcaact acgacgtgat tcccggcgtg
      841 gtctatacgc accccgaagt ggcgtgggtc ggcctcggcg aagaggagct caaagagaag
      901 ggcattgaat acaacgtggg ccggtcattc ttcaaaccca acggccgcgc gaaggcgatg
      961 atggaaggcg acggccagct gaagattttg gccgacaaga agaccgaccg catactgggc
     1021 gcgttctgcg tcggcgcgca ggcctcgcag cttattgcag agctcacgct cgcgatggaa
     1081 ttcggcgcgt catcagaaga cgtcgcacgc acctgccacg cacacccgac gctgtcagag
     1141 attgtgaaag atgccgcaga gagtgcgggg ggctgggcga tacaccaata ggtgtatcgc
     1201 cagatattat tgctttccaa ctttgataga catgcagctc ttgctgcatg tctatcaaaa
     1261 tctacggcat gtcctcttcg ggcaactgcc acaaaattcg cctcatcgtt aattacctga
     1321 aaatcccgca tgagtggatc gagaccgacc cgcggcacgg agcgaccaaa accgccgagt
     1381 tcaggcagat gaacccgaac gcgcatgtgc cggtcgtgca gctcgatgat ggcacgtatt
     1441 tgcccgaatc gaacgccata ctctattacc tggcgcaggg tagttcattc tggccgatcg
     1501 acaagttgtc gcaggcgcgc gtgctgcagt ggatgttctt tgagcagtac gaccatgaac
     1561 cgcgcatcgc cgttaaccgc agtatccaga gtgtcaccaa cacgctggca gaacgccgcg
     1621 ccgagtacga ggcgaatctg ccgaagggct accgtgcgct tggcgtcatg gagtctcacc
     1681 tcgcagaaca acgctggttc ggtgccgatg ttgcttcgat tgctgacatt gcgctctatg
     1741 cctacacgca tgtcgccgat gaaggtggtt tctctttggc ggattttccc cacatacggg
     1801 cctggctcgg cagaatggaa tctctcgaag ggcatagccg gatgaagttg tagtcagaat
     1861 aagaatgcgc gtagcgcgtt cttattctga caatttcaac tgctcgaggt ttcgcaggat
     1921 tacctcgatc tcttcgcgcg cttcgagcgg aaagatggat tcgcctgccg ggtattcact
     1981 gttgattgta taccagtcaa gtttcgccgt gacacactgg tgcttcaggg gaaagagata
     2041 gagtgtgccg atgcggtaga gcggtactgc ttccagttta tcttcgagta tggcgcggat
     2101 cgacataaac tcctgcgcgg ccgtgcctgc ctggcccggt agttttgcac acgcttcggg
     2161 tgtgactgta ccgcagagcc ttttgagcgc gagccggtcg aaggcacgtg cagtgtcgcg
     2221 cctgatctct ttatagaggc gcaatttctg ccgcatcagc gcgtcgtcgt catgccgttc
     2281 gaccttgaat tccatgatgc agtctttgcg cttgccgcta gtcttcgcgc gtttaagcag
     2341 cgttgacttc gagctggtca gatagtcctg cggctctact gccagcgcat agactggtgc
     2401 gacgcggcgc aataagacct gcctttcact ggtggtaaat ttccagcctg cgggcaagtg
     2461 gcgtgtgaaa aaacgctccg ccgctttcac attggccgag agtgtgcgct gcctgatgcg
     2521 ttcgcctacc ggtgactgcc gcatcagtat ctcggtctcg cttgcggagt gttcaggccc
     2581 ggggtgcgag ctttctactt gacccgtcac acggacgaac gggtagacga ccatgcaaca
     2641 gactatcgcc caccgaatga tcgcactttt agccatggcg gtctttccat tcgccctcgc
     2701 cgcgcaagac tcggtcaagc cgaaaaaggc cgccgaaaaa ccggcggcga aaaaagaaga
     2761 aactcccccc gcaaaagaag gcgagaagag cggtgcccgt gaggtgactt accttgacct
     2821 ggtgcacgcg cgcgataagg cgctgaaact gaaagaagac aacatgcgcg atattcagct
     2881 tttgaatgcg ctgggtgccg cgatgcccga ggctgtcgat aaaggagcat ccgaccagat
     2941 cgcgacagag tacaaagatg cgctcaagct gatctaccgc atggaatatg tcggtgcaga
     3001 aaagaccttg cgcacgaacc gcgagaacat tgcggccgcg atgggtaagg cgagtgaagt
     3061 tttccgcaaa aaaaccactg aaatgctcaa ccagagcgcc gaccgtatgg cagacctcga
     3121 catgcaggct gaaaatacgg gcagcgctga atccatccgg atggctggtg ttgtacaacg
     3181 caaccagcac cgcctgtcgg tcggctacca gcaactgacg ctcgcgtcga ccgctgagaa
     3241 gcaccgccgt tacgccgaag ccctttctca ctaccgcctg gcgcgcctgc acgcgattta
     3301 cctgcagatc gagctggcaa aagatgagaa cgaaaaaaac tccctgcgcg ccaagtatcg
     3361 caatgaactg gatgaaaaac ggatagttaa agaagaatca gcagaaaaag cagctccgcc
     3421 ggcacctgaa aagaagcctg caggcaagta aacggggtct cgcgccaggc aaaaaacgcg
     3481 tcgaaaaatt tcgtcatatc aactacattt tgcttgtcgc tatgtttccc gttgcgaagt
     3541 ctcccgtcac caacaaggcg gtgcaatggg gcagcaacaa gcaatcctct ttgcaaccgg
     3601 ggttctggtt tcggcctttt cggcgctttt agccggctgg ctggttaaga ccagaaacgg
     3661 tgcggggtgg gtgtactacg gtgcgattac cctgcgcatg cttgcattgc tggcaggttg
     3721 cacattggtg tacttgctgg cttcgccggg tgccggctcc ctgatcgggg cttatgcact
     3781 gggtgttgcg gcagcctggt tagttcacgt agcggtcgcg ttggtacagt taagagaatg
     3841 aaaaaacgaa acacagttct cgcaatcttc ctcggggcaa tcgccctcgt ttctttttgt
     3901 ccgactgctc tgtcggcaac tccagcccct gctaaggccg gagctcctgc tgctccagcg
     3961 actgcagttc cggctgctga tgctcacgct gccggtgaag cg
//
LOCUS       DAOQJF010000306         3983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_13858_length_3983_cov_4.879582, whole genome shotgun sequence.
ACCESSION   DAOQJF010000306 DAOQJF010000000
VERSION     DAOQJF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13858_length_3983_cov_4.879582"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLF85_13865"
     CDS             <1..614
                     /locus_tag="PLF85_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20804.1"
                     /translation="PTNGQQGSNQQQSKIEGFDGIAWGTTYKDMKERFRVMAANANAA
                     DNVEIVSDTPDREILIRRREIYYRYVFYKKPADKMVESMPKPTAGKPAEKPAADDPTA
                     GTAKFFFVESVFPLLKSEELYTKLTEKYGQRTGSSVGETNRGAYTWEKEEGFMVQWVE
                     PYAKGGYTRSLYYLSRKIRDEIKSDLQAWQFVREIKALENLLK"
     gene            616..1524
                     /locus_tag="PLF85_13870"
     CDS             616..1524
                     /locus_tag="PLF85_13870"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE20805.1"
                     /translation="MPARKIGVLINPASAGGRAVKAWPEFESLLAAGDFEIVSYTTSS
                     ETDFRVHARAFAGRFDIIGICGGDSSLTIAAEELFSAGFDGELVFLPAGSVNDIVLDI
                     REQRAPRASTLWLGEISAGATSKDFIGQANWGLGVAVNRQVGALLHSMPFLRPFTGII
                     GFLAIVFSHLLRRDLVSASIHLDSETLEGDYSIILATQIRHWASGLRFAPQADWYAPV
                     FEVVTIRRCSLVKLIRILLAARTARHVDFPEVEVHQSRRVKVSLASAAAVQVDGDILR
                     DVAGELKGLEYRLEKKKSRFVLSSAR"
     gene            complement(1521..2492)
                     /gene="fmt"
                     /locus_tag="PLF85_13875"
     CDS             complement(1521..2492)
                     /gene="fmt"
                     /locus_tag="PLF85_13875"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNE20806.1"
                     /translation="MTPTRIVFWGSPGIAATFLSHLVENHADKFTVTAVVTQTEKTIQ
                     RQGKTAARSAVHEKALALGLPVFTPRSVKKEAELLLGELNTIGYDLFVVLAYGKILPE
                     SIINAPRLKSVNFHGSLLPLLRGASPIEHALLHGFHETGWTLQRIVPELDAGDIVAQS
                     TVAIEHTETTGSLYTKLTADLLQHGAAMLSAYVNGKAQITPQDHGAATHCGKISAEDG
                     RMDFTRPAHEILNRYRAFTPRPGVFACFRGKKIKLAFDLTATPVALSQAAGSLIRPAK
                     DILLVTCGDGNALQLSALTPEGKKPMAVADFINGYRLAEGDCLTSSN"
     gene            complement(2489..3562)
                     /gene="hemH"
                     /locus_tag="PLF85_13880"
     CDS             complement(2489..3562)
                     /gene="hemH"
                     /locus_tag="PLF85_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNE20807.1"
                     /translation="MKKAATAKKKPAGSKAGKVTQKPGRQQKAVLLVNLGSPDSPSVP
                     HVRKYLKQFLMDGYVIDAPYPIRWMIVNLFVLPFRPKRSAEAYHKIWTKDGSPLLVFT
                     KLFAERLQKFSKHRIYWAMRYGHPSIASVLTLMRDAGHREVLLAPLYPHYALSTTVTV
                     VEEAKLHAQDLGITLSVLPPFYDSKAHQDVLARQVKKAAAGHDFVLFSYHGLPVSHIK
                     KADATGSHCLRNTNCWVVHSSAHTTCYRHQVIETTNTVARGAGLSADRFGFAFQSRLG
                     RAEWLAPNTVDFVGDLARKGIRKLAVIAPAFVADNLETLEEIDMQIREAFLTSGGEKF
                     TYLPCLNDNTEWVKGFAGLIDKA"
     gene            complement(3590..>3983)
                     /locus_tag="PLF85_13885"
     CDS             complement(3590..>3983)
                     /locus_tag="PLF85_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE20808.1"
                     /translation="NTPPTFGIYMLALTMEHIQETGGLKATEARNIAKAQTLYDAIDN
                     SAGFYRCPVNKADRSRMNVNYRIYKNDVADEALEKQFVNEAEKAGLSGLKGHRSVGGL
                     RASIYNAVEQNSINELVKFMQDFARKNG"
BASE COUNT          826 a         1095 c         1164 g          898 t
ORIGIN      
        1 agccgaccaa cgggcagcag ggtagcaacc agcagcagtc gaaaatcgaa ggcttcgacg
       61 gtatcgcctg gggcactact tacaaagaca tgaaggagcg ctttcgtgtc atggcggcca
      121 acgcgaacgc agccgacaac gtcgaaatag tctccgacac acccgatcgc gaaatactca
      181 ttcgccgccg cgaaatctac taccgctatg ttttctacaa gaagccggca gacaagatgg
      241 tcgagtccat gcccaaacca accgccggca agcctgccga aaaacctgcg gccgacgacc
      301 cgactgcagg tactgccaaa ttcttttttg tcgagagcgt tttcccgctg ctaaaatctg
      361 aagaactcta taccaagctg acggagaaat atggccagcg gacgggttcg agcgtcggcg
      421 agaccaaccg tggtgcctac acctgggaaa aagaagaagg cttcatggtg cagtgggttg
      481 aaccctatgc caagggcggt tatacccggt cgctctatta cctgtcgcgc aagatacgcg
      541 acgagatcaa gagcgacctg caggcctggc agtttgtccg cgagatcaaa gcgcttgaga
      601 acctgctgaa atagcgtgcc cgcgcgaaaa attggcgttc tcatcaaccc cgcttctgcg
      661 ggtggacgcg cggtcaaagc ctggcccgaa ttcgagagcc tgctggcagc aggcgacttc
      721 gaaattgttt cttataccac aagcagcgaa accgacttcc gcgtgcatgc gcgtgccttt
      781 gccggtcggt ttgatatcat cggcatctgc ggtggcgatt cgtcgctgac gattgcagcc
      841 gaagaacttt tttctgccgg ctttgatggc gagttggtat tcctgccggc tggttcggtg
      901 aacgacatcg tgctcgatat ccgcgagcag cgtgctccgc gggcatcgac gctctggttg
      961 ggtgaaatca gcgcaggcgc aacgagcaaa gattttatcg gccaggccaa ctgggggctc
     1021 ggcgttgccg tcaatcgcca ggtaggcgcg ctgctccaca gtatgccctt cttgcggccg
     1081 ttcacaggga tcattggttt tcttgcgatc gtcttcagtc acctgttgcg ccgcgatctc
     1141 gtcagcgctt ctatccatct cgactccgaa acgcttgagg gcgattattc gataatactg
     1201 gccacgcaga ttcgccactg ggcatcgggg ctgcgtttcg caccgcaggc agattggtac
     1261 gcgccggtct ttgaggtagt cactatcagg cgctgcagcc tcgtgaagct gattcgcatt
     1321 ctgcttgccg cacgcaccgc ccgccatgtc gattttcctg aagtcgaagt gcaccagtcg
     1381 cggcgcgtga aagtctcgtt agcctcggcc gctgccgtgc aggttgacgg tgacatcctg
     1441 cgcgatgtag ccggggaact gaagggcctg gagtacaggc tcgaaaaaaa gaaatcgcgc
     1501 tttgttctgt cgtcggcgcg ctaatttgaa ctggtgaggc aatcaccttc tgccaggcga
     1561 tagccattga taaaatctgc gactgccatt ggtttcttgc cttctggcgt tagtgcagaa
     1621 agctgcagcg cattgccgtc gccgcaggta accagcagaa tgtccttagc cgggcgaatg
     1681 agcgaacctg cagcctgtga gagcgcaacc ggcgtagccg taaggtcgaa agcaagttta
     1741 atctttttgc ccctgaaaca ggcaaacacc ccggggcgcg gggtgaaagc ccggtagcga
     1801 ttgagtattt cgtgcgcagg ccgcgtgaaa tccatacggc catcttctgc agatattttg
     1861 ccgcagtgtg tcgccgcgcc gtgatcttgt ggcgtaatct gcgccttgcc attgacatac
     1921 gcagaaagca ttgctgcgcc atgctgcagc aggtccgctg tcaacttcgt gtagagcgaa
     1981 cccgttgtct cggtatgttc gatagccact gtgctctgcg cgacgatatc gccggcgtcg
     2041 agttcaggca cgatgcgctg taaggtccag ccggtttcat ggaaaccgtg cagaagtgcg
     2101 tgctcaatcg gcgaagcccc gcgcagcaga ggcagcaggc tgccgtgaaa attgacggat
     2161 tttaagcggg gagcgttgat aatactctcg ggcaaaatct tgccgtatgc cagcacgaca
     2221 aaaagatcat agccgatcgt attgagctcc ccgagcagaa gctccgcctc ttttttcacc
     2281 gagcgcgggg taaagacggg taggccgaga gccagggctt tttcatgcac ggccgagcgc
     2341 gcggcagtct tgccctgccg ctggatcgtc ttttccgttt gggttaccac tgcggtgacc
     2401 gtgaacttgt ctgcgtggtt ttcgacgagg tgagaaagaa aagttgccgc gattccgggc
     2461 gaaccccaga agacgatgcg cgtcggcgtc atgctttgtc gattaggccg gcgaaacctt
     2521 tcacccactc tgtattatca ttcaggcagg gcaggtaggt aaatttttca ccgcccgagg
     2581 tgagaaatgc ttcgcgtatc tgcatatcga tctcttcgag agtctcaagg ttatcggcga
     2641 caaacgctgg ggcgatgact gccagcttgc ggattccttt tctggcgagg tcgcccacga
     2701 agtcgaccgt attcggggcc agccactcgg cccgacccag gcgcgactgg aaagcaaaac
     2761 cgaaacggtc tgcactcaag ccagccccgc gcgctaccgt attggtcgtc tcgataacct
     2821 ggtggcggta gcaggttgta tgcgccgacg agtgtacaac ccagcagttt gtattgcgca
     2881 ggcagtggct gccagttgcg tcagccttct tgatatgact gacaggcagg ccgtggtagc
     2941 tgaagagcac gaaatcatgg ccggcagccg cttttttaac ctgccgtgcc agcacatcct
     3001 ggtgcgcctt gctgtcatag aacggtggca ggacactcag ggtgattccc aggtcctggg
     3061 cgtgcagttt ggcttcttca acaacggtga cagttgtcga aagtgcatag tgtgggtaga
     3121 ggggggcgag cagtacttct ctgtgaccag catcgcgcat cagcgtcaat accgaggcaa
     3181 tcgatgggtg accatagcgc attgcccagt agatgcggtg tttgctgaac ttctgtagcc
     3241 tctcggcaaa aagtttggtg aagacaagca gcggcgaacc gtctttcgtc cagatcttgt
     3301 ggtatgcctc agcggagcgt tttggcctga aaggcaggac gaacaggttg acgatcatcc
     3361 agcggatggg gtaaggtgcg tcgatgacat aaccatccat caaaaactgc ttcaggtact
     3421 tgcgcacgtg aggcactgac ggcgagtcgg gcgaaccgag gttcaccagc aaaaccgctt
     3481 tctgctggcg acctggcttc tgggttacct taccggcttt gctgccagct ggctttttct
     3541 ttgcagtcgc tgcttttttc atgcggtcct gatcggcctt gtcgtggaat tagccgttct
     3601 tgcgcgcgaa atcctgcatg aacttcacga gctcgttgat cgagttctgc tcgaccgcgt
     3661 tgtagatcga agcgcgcaaa ccacccacag agcggtgtcc tttcaggccc gagagacctg
     3721 ccttctctgc ttcgttcacg aactgcttct cgagcgcttc gtctgccaca tcgttcttgt
     3781 agatgcggta gttcacgttc atgcgtgaac gatcggcttt gttaaccggg caacggtaga
     3841 agccggcaga gttatcgatt gcatcgtaca gggtctgcgc tttggcgatg ttgcgcgcct
     3901 ccgtcgcttt cagaccgcct gtctcctgga tatgctccat agtgagcgcg agcatataga
     3961 tgccgaatgt cggcggcgtg ttg
//
LOCUS       DAOQJF010000307         3955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14010_length_3955_cov_5.051282, whole genome shotgun sequence.
ACCESSION   DAOQJF010000307 DAOQJF010000000
VERSION     DAOQJF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14010_length_3955_cov_5.051282"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLF85_13890"
     CDS             complement(<1..214)
                     /locus_tag="PLF85_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNE20809.1"
                     /translation="MTLDELATEARGCKLCRLATTRKLVVFGEGNPKARLMFIGEGPG
                     KDEDASGRPFVGRAGQLLTRIIENGLK"
     gene            complement(218..970)
                     /locus_tag="PLF85_13895"
     CDS             complement(218..970)
                     /locus_tag="PLF85_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20810.1"
                     /translation="MSVTRRTLTRAIPVAIALGILLVFPRATCKGEGPKQVDGPTAQD
                     LGKKERDKFLAEATLNYPPYRSLRVAFTLKGKVDNQELYYEGELSATPTNLKIKLTDA
                     VFLSPLLTLEIGENKVTLKDHARNKTESIARADYQWVELFGRSFPVRFFEPLMRGFLP
                     GDASAKDTTFQNTAGGDTLARVNDGAFEAALYFNEAKLRKIFYRDKQRGEILVFEMSA
                     FFKNRTYPQKMRIEHSRSNDYLLLDFKGLRVN"
     gene            complement(1018..1923)
                     /locus_tag="PLF85_13900"
     CDS             complement(1018..1923)
                     /locus_tag="PLF85_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20811.1"
                     /translation="MKYKDFNEFKEAYKKRALSPEFKKSGLETLQKLHDEILDKPIKE
                     WLEIAAEVAPERNGENEWRAALEAIIKDIRPRSQAIIVKNGRTIKDIFESKSLDAWRK
                     VAQRPVDLPPQTVHEIMNQKAVKELFINIVHDAIIGFNKKFNPLAGAMAAFGMEKQIK
                     EFLVPFMDNAVKIGTDFVNNKANQPMFSDFAGKIFDIVIQQKPDILNSLMDKDREDEI
                     WQAIHITAQDKTFRKLAHEHALDVINDMKKDAGDKSLRQWLAERKLAIPFSAPTDKEL
                     ENVQKLLAESDACAQFMFTELSHHS"
     gene            complement(1920..>3955)
                     /locus_tag="PLF85_13905"
     CDS             complement(1920..>3955)
                     /locus_tag="PLF85_13905"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20812.1"
                     /translation="FSAYYSDKPVAAAKAFEEYLKIKGESEVALRYLARIALSGGDIQ
                     SATLYLERAVKADPDGVYSLQLLSEIYLKQNKLDDAARVLSLILKNDPLNERALQVMA
                     YIYQQKNDSRQAATYYKRLIIAVQKGTGNPDLLSQALYFLGNYYYQQDNFQRSLQYFR
                     KLHELDTDNARYLLIIGELQKITGQFRQSAETHSSLVTNQPDFAQAWESLAETWFILD
                     DPRAGEALKKFRALRKNEKPGILDGIEQQLAGKDDEALKAYDRIIGSNQNRLSARLGR
                     YRIYTKRKELTDARNEAFAVVVISQRLNGYDLARQYAHITLEHLNRQAQELNFREKFY
                     VPVTAMGTAANLDPQLEQLAIDFVELYITHASTLENTNERSVAVTWHELAARTISQLQ
                     LWYDAMLKNPAIRADTAKLEKVEKRVREAKNQLYQTRVNQAWTQVSIKGALADAEKNA
                     DAALALEQEYATAWFVKGIISNNYAEKKPAEYKVAAQYFLKAIEITEQKSKKKVAPAN
                     YHFYCGMALEKTNDFPAAEKHLKRTIELDPYNPTYLNYLGYMYSLRNQNLEEANALVL
                     RALEDDPENEAYLDTFGWIQYKLGNYREALEQLTVAASFAQKKNSVDPVIYFHLAEVH
                     YKMNNKVTALEYYVKTRNDIKRASEPLDAEYINNQIKKLESENKQSKKDEEQK"
BASE COUNT          733 a         1007 c         1105 g         1110 t
ORIGIN      
        1 gtttgaggcc gttttcgatg atcctggtta agagctggcc ggcgcggccg acgaatggcc
       61 tgccactggc atcttcatct ttgccgggac cttcacctat gaacatcagt cgtgcttttg
      121 gatttccctc gccgaagacc accagcttgc gcgttgtggc caggcggcag agtttgcagc
      181 ccctggcttc cgttgccagt tcatcgagtg tcatgattca gtttacgcgc aagcccttga
      241 agtccaggag caggtagtcg ttgctgcgtg agtgttcaat ccgcatcttt tgcgggtacg
      301 tacggttttt aaaaaaggcg ctcatttcaa aaaccagtat ttcaccgcgc tgcttgtcgc
      361 ggtaaaaaat cttgcggagt tttgcttcat taaagtagag tgcagcttca aacgcgccat
      421 cattgacgcg tgccagggta tctcccccgg ctgtgttctg gaaggtcgta tcttttgccg
      481 aggcgtcgcc gggtaaaaaa ccgcgcatca gcggctcaaa gaaccgcacg ggaaaagagc
      541 ggccgaagag ttcaacccac tggtagtctg cccgggcgat actttccgtc ttgttacgcg
      601 cgtggtcctt cagcgtgacc ttgttttccc caatctcaag cgtcagcagg ggcgaaagaa
      661 agacagcgtc ggtcagttta atcttcaggt tcgtgggggt tgcgctcagc tcaccttcgt
      721 aatagagctc ctgattgtct actttgccct tgagggtaaa agcgacgcgc agcgatcggt
      781 agggtgggta gttcagcgtc gcttcagcca ggaacttgtc gcgctccttc ttgccgagat
      841 cctgcgccgt cggcccatcg acctgcttcg ggccttcgcc cttgcaggtg gcacggggaa
      901 aaaccagcag gatacccagg gcaatagcga cgggtattgc acgcgtcaag gtgcgtctgg
      961 ttacgctcat cgccccctca tcctggcaac gctatctcag gcctgaaaac aaagacctca
     1021 gctgtggtgg ctgagttctg tgaacatgaa ttgcgcacag gcgtctgatt cggccagcag
     1081 cttctgcacg ttctcgagtt ctttgtctgt cggggcgctg aacggaattg ccagcttgcg
     1141 ctcggccagc cattgccgca ggctcttgtc gccggcgtcc tttttcatgt cgttgatgac
     1201 atcgagcgcg tgctcgtgcg ccagcttgcg gaaggttttg tcctgagcgg tgatgtggat
     1261 tgcctgccag atttcgtctt cacggtcttt gtccatcagc gaattgagaa tgtccggctt
     1321 ctgctggata acgatgtcga agatcttgcc cgcgaagtcg ctgaacatcg gttggttcgc
     1381 cttgttgttc acaaaatccg ttccgatttt gacggcattg tccatgaaag gcacaaggaa
     1441 ttctttgatc tgcttttcca tgccgaaggc agccatggcc ccggccagtg ggttgaattt
     1501 cttattgaag ccgatgattg catcgtgcac gatattgatg aagagttctt tcacagcttt
     1561 ctggttcatg atttcgtgca cggtttgcgg tggcaggtca accgggcgct gcgctacctt
     1621 tctccatgca tcgaggcttt tgctctcaaa gatgtcttta attgtccggc cgttttttac
     1681 gatgattgcc tgtgagcgcg ggcgaatatc cttgatgatc gcctcgagcg ctgcgcgcca
     1741 ttcattttcg ccgttacgct cgggagctac ttcggcggcg atttccagcc attctttgat
     1801 gggcttgtcg agtatttcgt catgcagctt ctgcaacgtt tcgaggcctg attttttaaa
     1861 ctcggggctt aatgcccgct tcttgtatgc ttctttgaat tcgttaaagt ccttgtattt
     1921 catttctgtt cctcgtcttt tttactttgt ttgttttcgg attccagttt tttaatctgg
     1981 ttgttgatat attcagcatc gagcggttca gacgcgcgtt tgatgtcgtt gcgtgtctta
     2041 acgtaatatt cgagcgcagt taccttgttg ttcatcttgt agtgcacttc ggccaggtga
     2101 aaatatatca ccgggtcaac ggagttcttc ttttgcgcaa acgatgcggc taccgtcagc
     2161 tgttcaagcg cctcgcgata attacccagt ttatactgta tccagccgaa ggtatcgagg
     2221 taagcttcgt tttcggggtc gtcctcgagc gcgcgcagta ccagagcatt cgcctcttcg
     2281 aggttctggt tgcgaagcga atacatgtaa ccgaggtagt tcaggtaggt cggattgtac
     2341 gggtccagtt caatagtccg cttcaggtgc ttttccgctg cggggaagtc gttggttttt
     2401 tcgagtgcca tgccgcagta gaagtggtag ttggcaggcg ccaccttctt cttgctcttt
     2461 tgttcagtga tttcgatcgc cttcaggaag tattgtgcgg ccaccttgta ttctgcaggt
     2521 tttttctctg cgtagttatt gctgattata cctttaacaa accaggcggt cgcatattcc
     2581 tgctcgagag caagtgcagc gtcggcattt ttttctgcgt cggcgagcgc tcccttgatg
     2641 cttacctgtg tccaggcctg gttgacccgg gtctggtaga gctggttctt cgcctcgcgt
     2701 actcgcttct ctactttttc cagcttcgca gtatcggcac gaatagccgg gtttttgagc
     2761 atggcgtcgt accacagctg caattgtgaa atggtgcgcg cggccaattc atgccatgtt
     2821 actgctacgg aacgttcgtt ggtgttttcg agcgtgctcg cgtgggtaat gtagagttct
     2881 acgaaatcga tcgccagctg ctcgagttgc gggtcaagat tggctgccgt gcccatggcc
     2941 gttaccggga catagaattt ttcgcggaaa ttcagctcct gcgcctggcg attcaggtgt
     3001 tcgagcgtaa tatgcgcgta ctggcgggcg aggtcataac cattgaggcg ttgggaaatg
     3061 acaacgaccg cgaaagcttc attgcgcgca tcggttaatt ctttgcgttt cgtgtagatg
     3121 cggtagcggc cgaggcgggc actcaggcgg ttttggttac taccaataat ccggtcgtat
     3181 gccttgagcg cttcgtcgtc tttgccagcg agttgttgct caataccgtc aagtataccc
     3241 ggtttttcat tcttgcgtag agcccggaat ttcttcagtg cctcgcctgc gcgcgggtca
     3301 tcgagaatga accaggtctc ggcgagggat tcccacgcct gcgcgaaatc aggctggttc
     3361 gtcacgaggc ttgaatgcgt ctctgccgac tgacgaaact ggcccgtaat cttttgtagc
     3421 tcaccgatga tcagcaggta acgggcgtta tctgtatcga gctcgtgtaa tttacggaaa
     3481 tactgcagcg agcgctggaa gttatcctgc tggtagtagt agttcccgag aaaatacagc
     3541 gcctgtgaaa gaaggtccgg gttaccggtg cctttttgca cggcgatgat cagccgctta
     3601 tagtaggtcg cagcctggcg ggagtcattc ttctgctggt agatatacgc catgacctgc
     3661 agcgcgcgtt cgttgagcgg gtcattcttg agaatgagag acagcacgcg tgccgcgtcg
     3721 tcgagtttgt tctgcttcag gtaaatttcc gaaagcagct gcagcgaata gacgccatcg
     3781 gggtcggctt tgaccgcgcg ttcgaggtag agcgtggcgc tctgtatgtc tccgcccgaa
     3841 agggcaatac gcgcgaggta gcgcagcgcc acttcgcttt ctcccttgat cttcaggtac
     3901 tcttcaaacg ctttggccgc cgcgacgggt ttgtcagagt aataggccga aaaag
//
LOCUS       DAOQJF010000308         3952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14030_length_3952_cov_5.970234, whole genome shotgun sequence.
ACCESSION   DAOQJF010000308 DAOQJF010000000
VERSION     DAOQJF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14030_length_3952_cov_5.970234"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /gene="speE"
                     /locus_tag="PLF85_13910"
     CDS             <1..809
                     /gene="speE"
                     /locus_tag="PLF85_13910"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNE20813.1"
                     /translation="RMLRLDGVFQTSDRDEFLYHEPLAHVAGITIDGPKKALVIGGGD
                     GGAAEEFLKYKTIEKVVMVELDGGVVDACKEHIPGICGNAFNDPKLELRIEDGIKYVF
                     ETKETFDQVLLDLTDPFGPSIELYTHEFYAQISKILSDKGILSLHIESPITRPELFSK
                     LYWTLKSVFPIVKPMTNYVPLYGTLWGFALASKTQDPTSVDKKTIIERLAKHQLPELH
                     FYNEDTHHSLFALPNYVREMIKAEQQPIRKGDTLPVPAGSKRNLAMYEAD"
     gene            984..1670
                     /locus_tag="PLF85_13915"
     CDS             984..1670
                     /locus_tag="PLF85_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20814.1"
                     /translation="MTVTISVAAIEESFKAGKDNQKAAELAETEKRLDDQIRQVSERL
                     QAMYTLRDIGPKVKQTPTQTIFSMGKDEDGEYIELVSYTFNPRSFNYGYPVGTAAKTM
                     RLYFAGKDLSKIKTIVDDQNFHEQYKYYTKAVHPGPTKGNPNDIQLATSFNKTTEVAE
                     KSPDYTVKLADVENDPTNPNRIKFKRDFYIENLIYFEKLFRFTFEFQKRGASNGDVET
                     INRLKHSLRY"
     gene            1848..2489
                     /locus_tag="PLF85_13920"
     CDS             1848..2489
                     /locus_tag="PLF85_13920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE20815.1"
                     /translation="MRVIAHDLNGPLTGMNLTLQAIRKLVKTTEMQKLVAGLEKDLTT
                     ATDLLEELKSLNRREIFRTVRLDLSREIAQFIEQVNANPRLPIIIDLAESDPKLVIAG
                     DLSRLRRVWHNIVRNVLDLAEARKGEERVTLTVSVKRTGQFARMRFLDDGGGIPRSKL
                     ARVFEPFYTTKGKLNRGLGLFIARKILTEHNGKIRIMSKKPFTRVTIDLPIVP"
     gene            2486..3562
                     /locus_tag="PLF85_13925"
     CDS             2486..3562
                     /locus_tag="PLF85_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE20816.1"
                     /translation="MSKAVSAPFLGLEVDGKKSSHRILLVEDVESIQLAIRDFLFSRY
                     DVQVASSAEQALMTLAMSPDLDLLITDIKLPEMNGLELGRLARKLRPNIPIILITSYD
                     VNEFIDVIREHGFSQIITKHGRMSLREIEVTIEKMLSGDIFGVKKYFPQLEEVEVGLR
                     NFPRPFANGVLYTTRITTLHERGEISDRIAAQLKSQKRASEATIKLVLDEITSNAMFR
                     APTNDIGEFKYQTKNQHEDILHTHQDIRLDEEDHFTLQFGICDEWIILCCIDPHGRLT
                     KKEILYRLHRHLSTDPETGMPAGLHDSHGRGIFLLREQLSSVVFNIDRGRRTEVICFY
                     NTTAAQAYKNISVYEIDTLHRNEH"
     gene            3559..>3952
                     /locus_tag="PLF85_13930"
     CDS             3559..>3952
                     /locus_tag="PLF85_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE20817.1"
                     /translation="MSNPIISLQDIRFAYGKRTILDGISLDFTPGSFSVIAGESGSGK
                     STLLYILGGFLKPDGGTYLFNGKPVYRRLGEFGLGRFRKNNIGFLFQDFRLLPFLTVE
                     QNIRFPSLFSGAALDKARLAERMQRLGIA"
BASE COUNT         1022 a         1126 c          969 g          835 t
ORIGIN      
        1 ggcgcatgct ccgccttgac ggcgttttcc agacctctga ccgcgacgaa tttctctacc
       61 atgaacccct cgcgcacgtc gccggcatca cgatcgacgg cccgaaaaag gcgctcgtca
      121 ttggcggtgg cgatggcggt gcagcggaag aatttctcaa gtataaaacc atcgaaaagg
      181 tcgtcatggt cgagctcgac ggcggcgtgg tcgatgcctg caaagagcac attcccggca
      241 tctgcggcaa tgcgttcaat gaccctaagc ttgagctgcg gattgaagac ggtatcaaat
      301 acgtgttcga aaccaaagaa actttcgacc aggtactgct cgacctgacc gacccgttcg
      361 ggccttcgat cgaactctac acccacgagt tctacgcgca gatcagcaag atcctcagcg
      421 acaagggcat tctcagcctc cacatcgaat cgcccattac gcgccctgag ctcttcagca
      481 agctgtactg gacgctcaaa tcggtcttcc cgatcgtcaa accgatgacc aattatgtcc
      541 cattgtatgg tacgctctgg ggatttgctt tggccagcaa gactcaagac ccgaccagtg
      601 tcgataaaaa gaccatcatc gaacgcctgg cgaagcacca gctgcccgag ctgcacttct
      661 ataacgaaga cacacaccat tcgctgtttg cactgccaaa ctacgtccgc gagatgatca
      721 aggcagaaca acagccaatc cgcaaaggcg ataccctgcc ggtaccggcc gggtcaaaac
      781 gcaacctggc gatgtacgag gcggattaac gggctgacga aaaactggcc ctttctacga
      841 catttttttc ccgtaactga aaatgttttc aggcaatttc agtttacggg gctgcaagtc
      901 gataaagaga ggggaattct cataaagggg actatactta tatgttgggc aaaacaaaaa
      961 agactctcgc tataattacc gcaatgaccg tgacaatttc ggtcgcggca atcgaagagt
     1021 cattcaaagc gggtaaagac aaccagaaag ctgcagagct ggcagagacc gaaaagcggt
     1081 tggacgacca gatacgccag gtcagcgagc gcctgcaggc aatgtacacg ctgcgtgaca
     1141 tcggccccaa ggtaaagcag actcctacac agaccatctt ctcgatgggc aaagacgaag
     1201 acggtgaata catcgaactc gtgtcatata ccttcaaccc acggtcattc aactacggtt
     1261 accctgtagg taccgcagca aaaacaatgc gcctgtactt cgcaggcaaa gacctgtcaa
     1321 aaatcaagac aatcgttgac gaccagaact tccacgagca gtacaagtac tacactaagg
     1381 cagtacatcc gggcccaaca aaaggcaacc cgaatgacat acagctcgca acctcgttca
     1441 acaagaccac cgaagttgct gagaagagcc cggactacac agttaaactg gcggatgttg
     1501 aaaacgatcc caccaacccc aaccgcatca agtttaagcg tgacttctac atcgaaaacc
     1561 tgatctattt cgagaagctg ttccgcttca cgtttgaatt ccagaagcgc ggcgcttcaa
     1621 acggcgatgt tgaaaccatc aaccgcctga aacattcgct gcgctactaa tctactgcgg
     1681 gtcgtatgac ctgcaaacac aagggggagc ttaagcctga cggcgaacgc tccctttttt
     1741 tatagggtat tccgacggct gcctctgcaa accaacgtcc cgaccgccgc cgcatcagcc
     1801 gcgaagaaaa acttttccag gcaaaattcg aatcgcttga gctttttgtg cgcgtgatcg
     1861 cgcacgatct caatggcccg ctcacgggaa tgaacctgac gctgcaggca atccgcaagc
     1921 tggtgaagac aaccgaaatg cagaaactcg tcgctgggct cgagaaagac ctcaccacgg
     1981 ccaccgacct gctggaagaa ttgaaatctc tcaaccggcg ggaaattttt cgtactgtgc
     2041 gcctcgacct ctccagggaa attgcgcagt ttatcgaaca ggtcaacgcg aatccgcgtt
     2101 tgccgataat cattgatctg gccgaaagcg acccgaaact ggtcatcgct ggcgacctgt
     2161 cgcgtctgcg cagggtctgg cataatattg tgcgcaacgt gctcgatctt gcagaggcgc
     2221 gcaagggcga agaaagggta actcttaccg tgagcgttaa aagaacgggc caatttgcac
     2281 gcatgcgctt tcttgatgac ggcggtggca ttccgcgctc aaaactggca cgtgtctttg
     2341 aaccttttta taccactaaa ggaaaactaa accgcggcct tggacttttt attgcgcgca
     2401 agattcttac cgagcacaat ggaaaaattc gcatcatgtc caaaaaaccc ttcacgcggg
     2461 tcacaatcga cctgcccatc gtaccatgag taaagccgtg tcagctccct ttctcggcct
     2521 cgaagtggat ggcaaaaaga gctcccaccg cattctgctc gtggaagacg ttgagtctat
     2581 ccaactcgcc atcagggatt ttcttttttc gcggtacgat gtgcaggtag cttcaagcgc
     2641 cgagcaggcg ctgatgacgc ttgcgatgtc acccgatctc gacctgctga ttacggacat
     2701 caagctaccc gagatgaacg gcctggagct cggccgcctg gcgcgcaaac tgcgtccgaa
     2761 tattcccata atactgatta cctcttacga tgtgaatgag tttatcgatg taatccgcga
     2821 gcatggattt tcgcagatca tcaccaagca tggccgcatg agcctgcgag aaatcgaggt
     2881 gacgatcgag aagatgcttt cgggcgatat tttcggcgtg aaaaaatatt tccctcagct
     2941 cgaagaagtc gaggtcggcc tgcgcaactt tccacggccc tttgccaatg gtgtgctcta
     3001 caccacgcgc atcacgacgc tgcacgaacg cggcgagatc agcgaccgca tcgcggcgca
     3061 gctcaaatcg cagaagcggg cttctgaagc gacaatcaag ctggtactcg atgaaatcac
     3121 gtctaatgcc atgttccggg ctcccaccaa cgatatcggg gaattcaagt accagacgaa
     3181 aaaccagcac gaagacatcc tccatacaca ccaggacatc cgcctggacg aagaagatca
     3241 cttcacgctg cagttcggca tttgcgatga atggatcata ctctgttgca tcgacccaca
     3301 tggccgcctg acgaagaaag aaatactcta ccgactgcac cggcatctct cgaccgaccc
     3361 ggaaaccggc atgcccgccg ggttgcatga ttcgcatggc cgcggaattt ttcttctgcg
     3421 tgagcaacta tcgtcagtgg tcttcaatat cgaccgcggt cggcgcaccg aggtgatctg
     3481 cttctacaat accacggccg cgcaggcgta taaaaatatt tctgtatacg agatcgatac
     3541 gttgcaccgg aacgaacatt gagtaacccc atcatttcat tgcaggatat ccgctttgct
     3601 tatggcaaac gcacaattct cgacggtatt tcactcgact tcacgcccgg cagcttctcg
     3661 gtcattgcgg gggagtcagg aagcgggaaa agtacactgc tctacatact tggcggcttt
     3721 ctcaaacccg acggcggcac ttacctgttt aacggcaaac ccgtataccg tcggctcggc
     3781 gaatttggcc ttgggcgttt tcgcaagaac aacatcggct tcctgtttca ggatttccgc
     3841 ctcttgccgt ttcttaccgt agagcagaat atccgctttc cgtccctgtt ttccggcgcc
     3901 gcgctcgaca aggccaggtt ggctgaacgc atgcaacgcc tcggcattgc cc
//
LOCUS       DAOQJF010000309         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14033_length_3951_cov_5.845739, whole genome shotgun sequence.
ACCESSION   DAOQJF010000309 DAOQJF010000000
VERSION     DAOQJF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14033_length_3951_cov_5.845739"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..514)
                     /locus_tag="PLF85_13935"
     CDS             complement(68..514)
                     /locus_tag="PLF85_13935"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNE20818.1"
                     /translation="MKEEEIQVFIRGTISYFEEVTGIKAELDIPYTKGPEQLLLDYSG
                     HIGISGARKGGLYITCPRGMLEDVTRKILLTDEVDDQSVFDMIGEIANTIAGNARTSF
                     GSEFNISVPTIFKGKPNNFRIVLRTPVVVLPINWKGYQAYLVVGID"
     gene            complement(529..888)
                     /locus_tag="PLF85_13940"
     CDS             complement(529..888)
                     /locus_tag="PLF85_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE20819.1"
                     /translation="MKLMIVDDSSIMRKAIEKYLTGAGIEIVGTAGDGKQAVELFKQT
                     LPDVVTLDITMPEMDGLQCLEELLKIKPDVKVMVVSALKDEATALEALKKGARSFLGK
                     PFTPESIKEAFDQVVAD"
     gene            1066..1632
                     /locus_tag="PLF85_13945"
     CDS             1066..1632
                     /locus_tag="PLF85_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HNE20820.1"
                     /translation="MAEMMNAKDFFGPLAALIGEWKGDKGMDIAPEPGGEEHSPYYET
                     ILIEPVGDATNAEEQTLSVLSYKQVVHRKSNGDVFHHQIGYWYFERKTGDILYSLMIP
                     RAVAVLARGKATESGEKTNISVKADAADKNYGIIESAFMSAKASTRAFTMDVSVAGNT
                     MTYKMNTGLHIYGKDFAHTDENTLTRSL"
     gene            1836..2462
                     /locus_tag="PLF85_13950"
     CDS             1836..2462
                     /locus_tag="PLF85_13950"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNE20821.1"
                     /translation="MKNNSLMMGVVAATMLLAACEKPPVAQAAGASDVLPVAKGEGKE
                     HQIDTAASKITWIGTKVTGKHNGTINLKSGSVFVKEGNVVAGKFVADMASIDDKDLSG
                     EYQAKLNKHLKSEDFFDVVKFPEGIFEISSVEKAANGVTIKGNLTLKGVTHGVTFDAD
                     IQSEKGVPKTAKANFNIDRKKWGIVYPGKPDDLISDTVNLTLDLKIKK"
     gene            complement(2528..3598)
                     /locus_tag="PLF85_13955"
     CDS             complement(2528..3598)
                     /locus_tag="PLF85_13955"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE20822.1"
                     /translation="MSQEQEQSQPTLLMVESEGGESRFIEHLVRNANAELIVLKGLRE
                     AISFVQNKKPDIVFVAGAFIEEAAALEALRSNCKAIGASVVLLAQNGTVTDTAAYDEV
                     LRTDATDQELTQRIRTLLDLKRARLEVEWMKQRLKNERRLLTKYFSEDIVEGILQDEH
                     ANSLSGRHQEATIMFFDVRKSTTIAENLEPQVFAEFLSEFFTDIMDLVYGNHGSVNKL
                     LGDGIMSTFGCPVPTANDVLNAAKCALQIRNHLATFNDVRPDYIKEPIRVGMGIATGK
                     VFAGNIGSVRRMEYTVLGDPVNLASRLESMTKEAGVDILIDGNTRHRLGNLIKCRKVQ
                     HQGVRGKLQEIEIFSLDELLKT"
     gene            complement(3595..>3951)
                     /locus_tag="PLF85_13960"
     CDS             complement(3595..>3951)
                     /locus_tag="PLF85_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE20823.1"
                     /translation="PETGKVELVSEKAAEGLRIYFRDDGRGLQLDKLRRKAIESGRHK
                     PEEVEKWDAQTVAGMIFVPGISTADATTLNAGRGVGMDIIQQKVESLGGKVDVRFESG
                     VFCEFAITLPGEAGVK"
BASE COUNT          963 a         1072 c          991 g          925 t
ORIGIN      
        1 aatcgaactg aaatagccgc tgatgcagcc gagaagtgcg gtaacgtctt cagcagtcag
       61 tctcaaatta gtctatcccc acgacgagat atgcctggta gcctttccag ttgatcggta
      121 acaccacgac gggagtccgc agaacaatgc ggaagttatt gggtttgccc ttgaaaatcg
      181 tcggtacaga aatattaaac tcagaaccaa acgaggtgcg cgcgttgccg gcaatcgtat
      241 tggcaatttc accgatcatg tcgaacacgg actggtcgtc aacttcatcg gtcagcagaa
      301 ttttgcgagt tacatcttcg agcatgccgc gcggacaggt gatgtacagg ccacctttac
      361 gggctcccga aatgccaatg tgtcccgaat aatcgagcag cagctgctcg gggccttttg
      421 tgtagggaat atcgagctcg gccttaatgc cggtcacttc ttcaaagtag ctgattgtcc
      481 cgcggatgaa tacctggatt tcttcttctt tcaccccact cccctgaatt agtcggcaac
      541 tacctgatcg aacgcttctt taatgctctc gggagtaaac ggtttaccaa gaaaacttct
      601 ggcacctttc ttcagtgcct cgagtgctgt ggcttcgtcc ttgagcgctg aaaccaccat
      661 aactttcaca tcgggcttta ttttcagcaa ttcttcaagg cactgcaggc catccatttc
      721 gggcatggta atatcgagcg tcacaacatc gggcagggtt tgtttaaaaa gctccactgc
      781 ctgtttgcca tctccggcgg ttccgacgat ctctatgcca gcgccggtga ggtacttctc
      841 aatcgctttg cgcataatgc tcgagtcatc gacaatcatc aacttcatag gtttaccttt
      901 tagcagagac cctgctgtca cctttttttc gattcaacgt tacaccctga accggggggt
      961 atgatgagtt gtttattcgc ttgaagagac ataaacgccg gtaacccggg cgttcagcgt
     1021 cccgagtcac tcttgccatc gtatgcattc gatttttgct ggcccatggc agagatgatg
     1081 aatgcgaaag attttttcgg gccgctggcg gcactgatag gcgaatggaa aggcgacaag
     1141 ggcatggata tcgctccaga acccggcggc gaagaacact ccccctacta cgaaacgatc
     1201 ttaatcgagc cggtaggcga cgccacaaat gcagaagagc agacgctgag cgtactttcc
     1261 tacaagcagg tcgtgcaccg caagtcaaat ggcgatgtct tccatcacca gatcggctac
     1321 tggtacttcg agcgcaaaac gggagacata ttgtactcgc tcatgatacc acgggcggtt
     1381 gcagtactcg cgcgcggcaa agcgaccgaa tcgggtgaaa agaccaacat cagtgtcaaa
     1441 gcagacgccg ccgacaaaaa ctatggcatt atcgaatcgg cattcatgtc cgctaaagct
     1501 tcgacgcggg cattcacgat ggatgtcagt gtcgcaggca acacgatgac ttacaagatg
     1561 aataccgggc tgcatatcta cggcaaagat tttgcacaca ccgacgaaaa taccctgacc
     1621 cgcagtcttt aattgcgggg taataattca tccatggcgt aaaaggccgt tggtgatttt
     1681 tcgccagcgg ccgctcattt gtttagatta agcccgcaac ccagacagca tgaatcttgg
     1741 ttttatccgg ggaaagctcg tcctcgccta atgccaaaac tcatgagccg ggttcgcccg
     1801 cgcccagcgc ggtaacaaat aacccgaggt aaaacatgaa aaacaattct ctgatgatgg
     1861 gcgtagtagc tgcaaccatg ctgctcgctg cctgcgaaaa accacctgtt gcacaggctg
     1921 ccggtgcgtc tgatgtactt cccgtagcga agggcgaagg caaagaacac cagatcgata
     1981 ctgctgcttc aaagatcacc tggatcggca caaaagtaac cggcaaacac aacggcacga
     2041 ttaacctgaa atcaggttcg gtattcgtga aagaaggtaa tgttgtggcc ggtaagtttg
     2101 tcgccgacat ggcttcgatc gacgataaag acctgagcgg cgaataccag gcaaaactga
     2161 acaagcacct gaaaagcgaa gatttcttcg acgtggtaaa attccccgaa ggaattttcg
     2221 agatcagctc agtagaaaaa gcagcgaatg gcgtgaccat taaaggtaac ctgacactga
     2281 aaggtgttac ccatggtgta acattcgacg cagatatcca gagcgaaaaa ggcgttccga
     2341 agacagcgaa ggccaacttc aatatcgacc gcaaaaagtg gggtattgtt tatccaggta
     2401 aacctgacga cctgatttca gacaccgtca acctgacgct cgatctgaaa atcaagaagt
     2461 aaagacgaca gggcagagca gcccgcaacc cgccgtgccc caggccggcg ggtttttttg
     2521 tgccttctca ggtcttcagc aactcatcaa gcgagaatat ctcgatttcc tgcagcttgc
     2581 cgcgcacgcc ctggtgttgt accttgcggc acttgatgag attacccagt cggtgccgtg
     2641 tattgccatc gatcagaata tccacacccg cctctttggt catggactcg agccggctgg
     2701 caagattcac cggatcgccg agtacggtat actccatgcg acgaacagac ccgatattgc
     2761 ctgcaaagac cttcccggtt gcaataccca tgccgactcg tatcggctct ttaatatagt
     2821 caggacgcac gtcgttgaac gtcgcgaggt ggttacgtat ctgcagggca catttcgcgg
     2881 cattgagcac atcatttgca gtgggtaccg ggcagccgaa agtcgacatg atgccatcgc
     2941 ccaacagctt gttcactgag ccgtggttgc cataaaccag gtccatgatg tcagtaaaaa
     3001 actcgctcag gaattcagcg aagacctgtg gttccagatt ttcagcgatc gtggtcgatt
     3061 tgcggacgtc aaagaacatg attgtcgctt cctggtgacg gccactgagg ctattggcat
     3121 gttcatcctg cagaatacct tcgacgatat cttcagagaa atactttgtc agcaggcgac
     3181 gttcgttctt caggcgctgc ttcatccact cgacttcgag gcgtgcgcgt ttcaggtcga
     3241 gcagcgtcct gatgcgctgc gtgagctcct ggtctgtggc atcggttcgc agcacttcat
     3301 cgtaggccgc ggtgtcagtc acagtaccat tctgcgccaa cagcacaacg ctcgccccga
     3361 tggccttaca gttggagcgc aaggcctcga gcgccgctgc ttcttcgatg aaggcgccgg
     3421 cgacgaaaac gatgtcgggt ttcttgtttt gcacgaagct gatcgcctcg cgcagccctt
     3481 tcagaacgat gagctctgca ttggcattgc gaacaaggtg ctcaatgaac ctgctctcgc
     3541 cgccctcgct ttcaaccatc agcaaggtag gttgggattg ttcctgttct tgactcattt
     3601 aactccggcc tcaccaggca gcgttatcgc gaattcgcag aagacgccac tttcaaatct
     3661 gacatccact ttgccaccga gcgactctac cttctgctgg ataatatcca taccaacgcc
     3721 gcgaccggcg ttaagtgttg ttgcgtctgc agtactgatg cccgggacga aaatcattcc
     3781 tgccactgtc tgcgcgtccc acttctcgac ttcttctggc ttgtgccggc ctgattcgat
     3841 cgccttgcga cgaagtttgt ccagctgcag gccacgaccg tcgtcgcgaa agtaaatgcg
     3901 caacccctcg gcagcttttt ccgagacaag ttcaacttta cccgtctcgg g
//
LOCUS       DAOQJF010000310         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14060_length_3945_cov_3.846272, whole genome shotgun sequence.
ACCESSION   DAOQJF010000310 DAOQJF010000000
VERSION     DAOQJF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14060_length_3945_cov_3.846272"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLF85_13965"
     CDS             complement(<1..205)
                     /locus_tag="PLF85_13965"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20824.1"
                     /translation="MKFSISGLRGVYPDDISPENWVEYVRAFASVLPAGPIALARDAR
                     ATGPALSRIAAGVLAVMQFVAVYN"
     gene            complement(241..573)
                     /locus_tag="PLF85_13970"
     CDS             complement(241..573)
                     /locus_tag="PLF85_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20825.1"
                     /translation="MKSPLRVLLIVYLAVLLVLALLPLTGYYAAALALPVMAVYLYRR
                     FIVEQATDGSVVTRGIFLFAALVFLCGFALNLFCTLYTTYRMADFRQGIEQGKKKIYS
                     ELKGEQNP"
     gene            complement(570..1043)
                     /locus_tag="PLF85_13975"
     CDS             complement(570..1043)
                     /locus_tag="PLF85_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20826.1"
                     /translation="MALVNLLLLALAAISLVFQLFTPVILNIPAIAVVLIAFALALLY
                     FRRRRRNTRLPVFAGLNLAVQTVFLLLSIAILVVITIPNESPEDSPAVVQGIRNILVS
                     QGWIAKPKNITLPATPKPQDEAANGSVNAPDASAVQITVEDAEKPAAKETEAQNP"
     gene            1227..2192
                     /locus_tag="PLF85_13980"
     CDS             1227..2192
                     /locus_tag="PLF85_13980"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE20827.1"
                     /translation="MTARFNENISRSLDLGLQEDYYSSLARLVRYRHKEKLIRELVNH
                     IGDSDRNMLTAEKKKSEMLLQTILPVSMIAELKEKGSVTPTHHENAIVLMTDFVGFTD
                     LARYMSPRELLKKLSVYFEEFECILDRYRIEKVKTIGDALLAVGGLQPGNKTAMVDCI
                     LACMEMLAFVEKQRAEALARGEEAWGMRCVLHSGSVVSGVVGHSKIAFDIWGFAVNVA
                     SRIEALAPEGKITVSEAVYHRIRDFFDCDFAGEQELKGVGNRRIYSVSGFKPRLRHEA
                     ATPYVPNHIFGQLYGHMKAGDHIVYSDGKYRVFRRQKTTALTQAS"
     gene            2202..2996
                     /locus_tag="PLF85_13985"
     CDS             2202..2996
                     /locus_tag="PLF85_13985"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20828.1"
                     /translation="MEETQTSLEAVELQIDTTHAVEIAKNVYWIGFYDEEEKLHCNPF
                     LIKNGPETILIDPGSLPHFPVVARKAFALASADSISSIVLQHQDPDLCAAVPIFEDLR
                     ENAQMDIYCAVGTSYLIHHYGIKGTYRTIPNKIGAYNLKTKGGRDLDFYWTPYAHSFG
                     AMMTHDKATNTLFTSDILGGVGSSWALYHDQPALDNMKAFMQLIMPSNKILRYALQMI
                     RDIDPEIAAPQHGQILRRRDIAEVIAFLWDLPCGIDLIDDPRLKTV"
     gene            complement(3068..>3945)
                     /locus_tag="PLF85_13990"
     CDS             complement(3068..>3945)
                     /locus_tag="PLF85_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20829.1"
                     /translation="YIFVILPLALATVVYSGEWDASAGFGGTYLNSGGNNAETFYWGP
                     FASVGNQLTENLHVRADLRHSRGRLLYDGLGGIARQSWTAIGPGVNWDFAEGWNLDGD
                     YKFRIGENNFREHEFAAALEYAALSKVRFAGDIVYGTRGYTFPRTGTDISAQSASYTL
                     EVVPRPVDWLEFPVSGNIASSSYNTNKSAYTVYMLSLGASVFLADRQWKIGAAITPGK
                     DSSNYSIIGGDLRIRYRATDNIALRFVASVTSYSFSQTQTAKSKRSSGTAVNPLGNSD
                     SFNITAVGLEASYSY"
BASE COUNT          992 a         1081 c         1047 g          825 t
ORIGIN      
        1 cattataaac tgcaacgaac tgcattacgg cgagaacacc ggcggcgatg cggctcagcg
       61 ccggcccggt ggcgcgtgcg tcgcgggcaa gagcgattgg cccggcaggt agtaccgacg
      121 cgaacgcgcg gacatattcc acccagtttt ccggtgatat gtcatcaggg taaactccgc
      181 gcaggcccga gatcgaaaat ttcaaagtct gcgaagtgct gagtaaacgg gcaacattgc
      241 tcatggattt tgttcacctt tgagttcaga atagattttc ttttttccct gttcaatacc
      301 ctggcgaaag tcagccatgc ggtatgtagt gtagagtgtg cagaacaggt tgagggcaaa
      361 accacacaga aagaccagcg cagcaaaaag aaaaatcccc ctggtcacca cgctgccgtc
      421 agtggcctgc tcgacgatga agcggcggta cagatagacc gccatcaccg gcagtgcaag
      481 ggctgcggca taataccccg tcagcggcag caaggccagg acaagtagta cggccaggta
      541 aacaatcagc aaaacacgca gcggggattt catgggttct gcgcctcggt ttcttttgct
      601 gcaggcttct ctgcatcttc gacggtaatc tgcactgcag atgcatcagg tgcattgacg
      661 ctcccattgg ctgcttcatc ctgcggcttt ggggtagccg gcagggtgat gtttttgggc
      721 ttagcaatcc acccctggct aaccaaaata ttacgaatgc cctgcacgac agccggtgaa
      781 tcttccggcg attcattggg gatagtaatg acaaccaaaa tagcaatact aagaagaaga
      841 aaaaccgtct gcaccgccag gttaagcccc gcaaaaaccg gcagccgggt atttctcctg
      901 cgccgcctga aatagagcaa ggccagggca aaagcgatca ggacaacagc aattgccgga
      961 atattgagaa taaccggggt aaatagctgg aagacaagcg aaatcgcggc taacgcaagc
     1021 agcagaagat taacaagcgc cacgggaaaa cctctgaaac cgaccggggt cgtctatcgc
     1081 gaaaaaagtg gccggtccgt gccccggcac ctgacgccca cctgtcgccg agtaggtata
     1141 tctacgtaga cgtgacggca ggattaccca tagctatttt ttcctatagc ggttattgac
     1201 ttagatcaag gaattggagt gtacgattga ctgcccggtt taacgaaaat atctcccgct
     1261 cgctcgacct ggggctgcag gaagactact atagctcact cgcacgcctg gtacgctacc
     1321 gccacaagga aaagctgatt cgggaactcg tgaaccatat cggcgactct gaccgtaaca
     1381 tgctcaccgc cgaaaaaaag aagagcgaga tgctgttgca gacgatcctg ccggtttcga
     1441 tgatcgcaga gctcaaggaa aaaggttcgg tcacccccac ccaccatgag aatgcgatcg
     1501 tgctgatgac cgacttcgtc ggttttaccg acctggcgcg ttacatgagc ccgcgcgaac
     1561 tgctgaaaaa gctctctgtg tattttgaag aattcgagtg tattcttgac cgctaccgca
     1621 tcgaaaaggt caaaacgatt ggcgacgcgc tgctcgcggt cggcggtctg cagcctggca
     1681 ataagactgc catggttgat tgtatcctcg cgtgcatgga gatgctggcc ttcgtcgaaa
     1741 agcagcgcgc cgaggcactc gcgcgcggtg aagaagcctg gggcatgcgc tgcgtgctgc
     1801 attcaggttc tgtcgtttct ggcgtggtag gccacagcaa gatcgcgttc gatatctggg
     1861 gtttcgccgt caacgtggct tcgcgtatcg aggcccttgc ccctgaagga aaaattaccg
     1921 tcagcgaagc ggtttaccac cgcattcgcg attttttcga ttgtgatttt gccggtgaac
     1981 aggaactgaa aggagtcggt aaccggagga tttactccgt ttcaggcttc aagccacgcc
     2041 tgcggcatga agccgcgacg ccatacgtac ccaaccacat attcggccag ctctacggcc
     2101 acatgaaagc cggcgaccac atagtttaca gcgatggaaa ataccgtgtc ttcaggcgac
     2161 aaaaaacgac ggcattgaca caggcaagtt aaggattttt tatggaagaa acacaaacca
     2221 gccttgaggc agtcgaacta cagatagaca cgacgcatgc cgtagaaatt gcgaaaaatg
     2281 tctactggat cggtttttac gacgaagaag agaagctcca ctgtaacccg ttcctgatca
     2341 agaatggtcc ggaaaccatt ctgattgacc ccggttcact gccacatttt cccgtagtcg
     2401 cccgcaaggc tttcgccctg gcgagcgccg attccatttc ctcgattgtc ctccagcacc
     2461 aggatcccga tctctgcgcc gcggtaccga tattcgaaga cctgcgggaa aatgcgcaga
     2521 tggacatcta ctgcgccgtc ggcacgagct acctgatcca tcattatgga atcaagggta
     2581 cataccgtac gatccccaac aaaattgggg cctataatct gaagaccaag ggcggccgcg
     2641 atctcgactt ctactggact ccttacgcgc attcattcgg cgcgatgatg acccacgaca
     2701 aagccaccaa taccctgttc acgagcgaca tcctgggggg cgtcggcagc agctgggcgc
     2761 tctatcatga ccagccggca cttgataaca tgaaagcttt catgcagctg atcatgccct
     2821 cgaataagat actgcgttat gcgctgcaaa tgatcagaga tattgatccc gaaattgctg
     2881 caccacagca cggccagata ctccgcaggc gcgacatcgc cgaggtgatc gccttcctgt
     2941 gggaccttcc ctgcggcatt gatctgattg acgacccgcg gctgaaaact gtgtaagcgc
     3001 agcgccgtga tcgactgaca ctggcaagcg ccgcaatcta ccagcaggtt cctttttcag
     3061 gagcctgcta gtagctgtac gaagcttcga gcccgacagc agtgatgttg aagctgtctg
     3121 aatttcccag tggattgact gcggtacccg aagaacgttt cgatttagcc gtctgcgtct
     3181 gcgaaaaact atagctcgtc acactcgcga caaaccgcag ggcgatatta tcggtagcgc
     3241 ggtagcgtat gcgcaggtcg ccgccaataa tgctgtaatt cgatgaatct tttccaggtg
     3301 tgatagctgc gcctatcttc cactgcctgt cggcaagaaa gaccgaagcc cccagcgaaa
     3361 gcatgtagac cgtatacgct gacttgttcg tgttatagct tgacgaagcg atgttcccgg
     3421 aaaccgggaa ttcgagccag tcaacgggac gaggcactac ttcgagcgtg tacgaggcag
     3481 actgtgcaga aatatctgta cccgtccggg gaaaagtata gccgcgcgtg ccgtaaacaa
     3541 tgtcgcccgc aaagcgcacc ttgctgagtg ccgcgtattc aagcgcggca gcaaattcat
     3601 gttccctgaa attattctcc ccgatgcgga atttatagtc gccgtcaaga ttccagccct
     3661 cggcaaaatc ccagttcacc cccgggccaa tcgcggtcca cgattgcctg gcgattcccc
     3721 ccaagccatc atagagcaga cggccgcgtg aatggcgcag atctgcccgc acatgcaggt
     3781 tttctgtcag ctggttacca acagaagcaa atggtcccca gtaaaaggtt tctgcattgt
     3841 tgccaccgct gttaagatac gtaccgccga agccggcaga tgcatcccac tcaccggaat
     3901 agacaacggt agccagggca agcgggagaa taacaaatat gtagc
//
LOCUS       DAOQJF010000311         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14069_length_3944_cov_4.218051, whole genome shotgun sequence.
ACCESSION   DAOQJF010000311 DAOQJF010000000
VERSION     DAOQJF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14069_length_3944_cov_4.218051"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLF85_13995"
     CDS             complement(<1..591)
                     /locus_tag="PLF85_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE20830.1"
                     /translation="MEFLKRFDLDWANVKTVFRRELANYFNTPIGYIFLGIFVILVNF
                     LFFFITRFFDRGYSLQGLFDMLKLIYLAFIPVITMRLWAEERRSGTVELLLTLPFSEL
                     ELILGKYFSAFIFLGIALATTLVLPLIAFYVSAPDFMVLLGSYLGAWLSGSAYIAMGL
                     YISWLTRDQISAFLLSFLACLFVYLLGYQPVLQFFGP"
     gene            complement(609..1556)
                     /locus_tag="PLF85_14000"
     CDS             complement(609..1556)
                     /locus_tag="PLF85_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE20831.1"
                     /translation="MISVTGLKKNFGDYEAVKQISFSIGKGEVVALLGPNGAGKTTTM
                     RMLTGFYEPTAGSATIGGHDVQKDRRAVQSLIGYLPESASSYPDMLVSEFLQFIAQSR
                     GLSAEQTEKGMEKAVKVTDLTKYLYTPIMHLSKGYKQRVGLAAALLHDPQVLILDEPT
                     SGLDPNQINEIRQLIRELSKEKTIILSTHILKEVEDTCQRAIIIADGRIVLDDKLEKI
                     QRMKEGREMWEFALKGNATASLPKIQSLLGSDESVSVISGNDIETRFAAETTSGFNER
                     LFALAVSEKWTLREMFMQKQSIEDVFARLTAKATDGQGQ"
     gene            1758..>3944
                     /locus_tag="PLF85_14005"
     CDS             1758..>3944
                     /locus_tag="PLF85_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE20832.1"
                     /translation="MPEIAGIAIDAADISPAEKGLVFHQELVLTETTLKNLQKILYPL
                     IEGQNLLLVGDAGVGKNALIYYINRIRSLPTIRFSFNQDTLPEDLSGSFRVLPDGFQW
                     NNGPLTEALEKGYTFVADEMNLASPEILKRFISVFDRRRLALLEKDGSEIVAHERFSF
                     VATQNPARGFEGRKNLPESIARHFTTIYLDHYPAEEEKQILRGLYPEEYGTVADTIVL
                     LQRTLEAAIWKNELAKDDLEHYHFNLRTAQRAMNRAVIAGIADIQSPAFRDLLFTNYV
                     NVFRLEADREQALKLAAQLMEYEPAALKRDHEIFTAAESEANYLAAGGEGNRGQRLYF
                     PVTRSRARLLEQIEASLTASDNLLLEGDDAARILELCHAWAEHNNRRITEIFLSRGMH
                     TSDIIGALRPHGTSVRWVDGPLTAGLRRGDLIILENIDAAGSELIEKLNMLLDHAGRL
                     ALPPEAEGDSILRKEGECRVIAIKRTRRSRNQQTISRALRNRFFNLHVPVLESQQEIH
                     EVITAVLAHEFGEDSSPLRQTLVQKLTLFHTKADEAAKEKKLGENLAETIRYREEHML
                     RLLAHASRFVAAGDALETMKRGVDIYYAGMLVSNDDRVQIRRLFDRIFSDLPWEDIFA
                     SLDEGKKKVLSKRAQGRDKKIAPPDWDKKKHFREALTGKAEKRLDGKELKKGLRIDTP
                     ETGGNIKEGPDAWYGSDTQGNGGQGEPAGGGGAWGYRTEQLFQDFLK"
BASE COUNT          943 a         1132 c         1084 g          785 t
ORIGIN      
        1 ggggccaaag aactgcagca ccggctggta tcccagcagg taaacgaaga ggcaggccag
       61 aaagctcagc aaaaaggcgc tgatctggtc acgcgtcagc cacgagatgt agagccccat
      121 cgcaatatag gccgaacccg aaagccatgc cccaaggtac gaaccgagca gcaccatgaa
      181 atcgggtgct gagacgtaga aggcgatcag ggggagtacc agcgttgtcg cgagtgcaat
      241 gcccaggaag ataaacgccg aaaagtactt acccaggatc agctcgagtt cgctgaaggg
      301 cagggtgagc agaagctcta ccgtaccgct gcggcgttct tcggcccaca ggcgcatcgt
      361 gatgaccggg ataaacgcca ggtagatcag tttgagcatg tcaaacagcc cctgcagcga
      421 gtagccgcgg tcaaagaagc gcgtgatgaa gaaaaacagg aagttcacga gaatcacgaa
      481 tatcccgaga aagatgtagc cgatgggcgt attgaagtag tttgccagct cgcgccggaa
      541 aaccgtcttc acattagccc agtcgagatc aaaacgtttg aggaattcca tatttgctcc
      601 gttaatcctt attgtccctg gccatcagtg gccttggctg ttagccgcgc gaacacatct
      661 tcaatacttt gtttctgcat gaacatttcg cgcagtgtcc atttttcgct caccgcgagt
      721 gcaaagagcc tctcgttaaa tccactggtg gtttcagccg caaaacgcgt ttcgatgtca
      781 ttgcctgaaa tcactgagac actttcgtcg ctgccaagca gggactgtat cttaggcagc
      841 gacgcagttg cattgccttt gagcgcgaac tcccacattt cgcggccttc tttcatgcgc
      901 tgtatcttct cgagcttgtc gtcgagcacg atgcgcccgt cggcgatgat aattgcacgc
      961 tggcaggtat cttcaacttc tttcaggatg tgagtactga gaatgatcgt cttctcttta
     1021 gagagctcgc gtatcagttg ccggatctcg ttgatctggt tggggtcgag gcccgaggtt
     1081 ggctcgtcca aaatcagcac ctgcgggtca tgcagcaggg cagcggctaa tcccacgcgc
     1141 tgcttgtaac cttttgacag gtgcatgatg ggagtgtaga ggtacttggt aaggtctgtc
     1201 accttgactg ccttttccat acccttctcg gtctgttcgg ccgagaggcc gcggctctgt
     1261 gcaataaact gcaaaaactc agagaccagc atgtcggggt atgaactcgc gctttcgggc
     1321 aggtaaccga tcagcgactg cacggcgcgg cggtctttct gcacatcatg gccgccaatc
     1381 gtcgcgctgc ccgccgtcgg ctcatagaac ccggtcagca tgcgcatggt cgttgtctta
     1441 ccggccccgt tggggcccag cagggccacg acctcgccct taccgatcga aaaactgatt
     1501 tgtttcacgg cttcatagtc gccgaaattc ttctttaaac ccgttactga aatcataggc
     1561 tatacccaaa aaggaaaaat tgcccgtcaa gcacatagtt ggcactcaat acaccacagt
     1621 gctaagaccc aggcaaggca cacctccggt gtgccttgcc tgggtcttga actcgtttac
     1681 atccgattgc ggatgcaaac cgtgccgcaa gccatcgtgc atatacacgc gaggccgctg
     1741 cacagcaagg aaacaacatg cccgaaatcg ccggaatcgc catcgacgcc gcggatatat
     1801 ctcccgccga gaagggactg gtttttcacc aggagcttgt tctcacggag acgacactca
     1861 agaacctgca gaagattctc tacccgctga ttgaagggca gaacctcttg ctcgtcggcg
     1921 acgccggcgt cggcaaaaat gcacttatct actatatcaa ccgtattcgc tcgctaccga
     1981 cgatacgctt cagcttcaac caggatacgc tgcccgaaga cctttcggga tcgttccggg
     2041 ttttgcccga cggcttccag tggaataacg ggccgctaac cgaagcgctc gaaaaaggtt
     2101 acacctttgt cgccgatgag atgaaccttg cttcgccgga aattctcaaa cgcttcatca
     2161 gcgtctttga ccgccgccgc ctcgcgctgc tcgaaaagga tggcagcgag atcgtggcgc
     2221 acgagcgctt ctctttcgtc gcgacacaga acccggcgcg tggctttgaa ggccgcaaga
     2281 acctgcccga atcgatcgcg cggcacttca cgaccatcta tctcgaccat tacccggcag
     2341 aagaagaaaa gcagattttg cgggggctct atcccgagga atacggtacg gttgccgaca
     2401 caattgtcct gttacagcga acactcgaag ctgcgatctg gaagaacgaa ctggcgaagg
     2461 acgatctcga gcactaccat ttcaacctgc gtacggcgca gcgcgccatg aaccgggcag
     2521 tgatcgcggg tatcgcagac atccagtcgc cagcctttcg cgacctgctt tttaccaact
     2581 atgtcaatgt ctttaggctc gaagcagatc gtgagcaggc cttaaagctt gcggcgcaac
     2641 tcatggaata tgaacccgcg gcgctcaagc gcgaccatga gattttcacg gctgcagaat
     2701 ctgaagcgaa ttacctcgcg gccggtggtg aaggcaaccg cgggcaacgg ctgtatttcc
     2761 cggtaacgcg ctcacgcgcg cgcctgcttg agcagatcga agcttcgctc acggcaagtg
     2821 acaacctgct gctcgagggc gacgacgcgg cgcgcattct cgaactctgc catgcctggg
     2881 cagaacacaa taaccgccgc ataaccgaaa ttttcctgtc gaggggcatg cacaccagcg
     2941 atataatagg agctctgcgt ccgcacggta cttcagtccg ctgggtagat ggcccgctga
     3001 ctgcgggatt gcgccgcggc gacctgatta tactcgaaaa catcgatgct gcgggttcag
     3061 agctgatcga aaaactcaat atgctgctcg accatgcggg gcgcctggcg ctgccaccag
     3121 aggcagaagg cgattcgata ctgcgcaaag aaggtgaatg ccgcgtgatc gcgatcaagc
     3181 gcacgcgccg cagccgcaac cagcagacta tctcgcgcgc gctcaggaac cgattcttca
     3241 acctgcacgt gcccgtgctc gaaagccagc aggaaatcca tgaagtgatc accgcggtac
     3301 tcgcacacga gttcggtgaa gacagctctc cactcaggca gacgctggtg caaaagctca
     3361 cgctcttcca taccaaggca gacgaagccg caaaagagaa aaaactcggc gagaacctgg
     3421 ccgaaacgat acgctaccgc gaagagcata tgctccgcct tcttgcgcat gcatcgcgtt
     3481 ttgtcgctgc gggcgatgcg ctcgaaacca tgaagcgcgg cgttgacatc tactatgccg
     3541 gtatgctcgt cagcaacgac gaccgcgtgc agattcgccg cctgttcgac cgtatctttt
     3601 ctgacctgcc gtgggaagac atctttgcct cgctggacga aggtaaaaaa aaagtcctga
     3661 gtaaaagggc gcagggccga gataagaaga ttgccccccc cgactgggat aaaaaaaaac
     3721 actttcgtga agcgctgacc ggcaaagccg agaagcgcct cgatggcaag gagctcaaga
     3781 agggcctgcg catcgacacg cccgaaaccg gcggcaatat caaagaaggc cccgacgcgt
     3841 ggtacgggtc agacacgcag ggcaatggcg ggcagggcga gcccgcaggt ggcggcggtg
     3901 cctggggcta ccgcaccgag cagctgttcc aggatttctt aaaa
//
LOCUS       DAOQJF010000312         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14086_length_3941_cov_4.649768, whole genome shotgun sequence.
ACCESSION   DAOQJF010000312 DAOQJF010000000
VERSION     DAOQJF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14086_length_3941_cov_4.649768"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLF85_14010"
     CDS             <1..214
                     /locus_tag="PLF85_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNE20833.1"
                     /translation="TGVIQVTKEEAFEYTKRAAKEEALFVGISSGASLAAVAKKIPEL
                     KKGSRILTFNYDTGERYLSIEGLFQL"
     gene            complement(241..993)
                     /locus_tag="PLF85_14015"
     CDS             complement(241..993)
                     /locus_tag="PLF85_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE20834.1"
                     /translation="MVETRYREQVYEIILNRPEKHNAFDAEMIGRVTQAVHDVPAETR
                     LVLFRGNGKSFCAGADINYMSQQASFTDDENREDARRLAHMFEMIYGLPQPTVALAQG
                     NIFGGGLGILAACDIALATSDARFCFSEVRLGIIPAIISTYVLRKTGTAFAKRYFLSA
                     EIFDADTARASGLIADHFAPEQAEEACTRLCDTLVSQGLQAQKSIKKVLHAYENFEPF
                     SRDQLVHELAKLRASDDARMRMASFLDRNKRS"
     gene            complement(1023..2666)
                     /locus_tag="PLF85_14020"
     CDS             complement(1023..2666)
                     /locus_tag="PLF85_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNE20835.1"
                     /translation="MPTFETKVSSQSPEFKENAAAYGRHLDSLAALRKRIAPESRQAA
                     VAKHTERGKLFARDRITGLLDAGSPFFEIGAFAGLDLYPGEEIPGAGAVAGIGFVHGR
                     MVAVLANDATVKGGTYYPITVKKHLRLQEFAQLNRLPCIYMVDSGGAFLPMQDEVFPD
                     RDHFGRIFFNQARMSAAGIPQIAAVMGSCTAGGAYVPAMCDETIIVEKHGTIFLAGPP
                     LVKMATGEEVSAEDLGGARLHTSRSGVADHYALDDFDALRIAREIVATLPRPEGNALA
                     QGRRTSLAEPVLSVEEIPGIIPADTRKPFDVREIIARIVDGSSLQEFKAEYGKTLVCA
                     FAEIGGFRVGILANNGVLFSESALKGTHFIELCCQREIPLIFLQNITGFMVGKKYEEG
                     GIAKDGAKMVSAVSNANVPKFTVIIGGSYGAGNYGMCGRAYSPDLLLMWPNARISVMG
                     GEQAAGVLAIVREQSARAQAKKSGKLHDEAGLGRELDEIRQKITAQFDGQSTASYSSA
                     RLWDDGVIDPRETRRALIMGLLAAAGRPLQAFTPGVYRM"
     gene            complement(2790..>3941)
                     /locus_tag="PLF85_14025"
     CDS             complement(2790..>3941)
                     /locus_tag="PLF85_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNE20836.1"
                     /translation="WFLPVGILIATAAAIIASQAIISGAFTLIAEAIRLNLWPKVRIK
                     YPSILKGQIYVPSANRLLWVGCIGVTLFFGKAANMEAAYGLAITLDMIMTTMLLSVFL
                     YSRSSSMLVAVVTLVSFLAIEASFLVANLGKFSHGGWVTGVMGFGIFTVIWSWYSARK
                     IRNRYTEFVRLEEYYDTLHNLSHDSSVSKYSTNLVYMTSANNKYEIEWKVIYSLLYKQ
                     PKRADIYWFVHVDVTDEPYTRECVVDVLVPNDVIRVDFKLGFRVEPRINQYFRKVVTE
                     LVQRGIVDITSRYSSLHEKNIIGDFRFVVLDRVLTHDNELNFYEKIVTAMHKLWKAMC
                     PGEEKAFGLDTSIVEVERVPLIVSKPREFSLDISLSSDTAEKNKIAARS"
BASE COUNT          923 a         1128 c         1059 g          831 t
ORIGIN      
        1 cacaggagtt attcaggtaa ccaaagaaga agccttcgaa tacacaaaac gcgctgccaa
       61 agaagaagcg ctctttgtgg gaatttcgag cggtgcgtcg ctggcagccg tggcgaaaaa
      121 aatacccgag ctgaagaagg gatcacggat actgaccttt aactatgaca ccggcgagcg
      181 ttacctttca atcgaaggcc tgttccaact ctaacaggct cctgaaaagg agccagctaa
      241 tcaagaccgc ttgttgcggt cgagaaaact ggccatgcgc atgcgcgcgt catcggacgc
      301 gcgcaatttt gccagttcgt gaacgagctg gtcacggcta aatggttcga agttttcgta
      361 agcgtgcagc accttcttga tactcttctg tgcctgcagc ccctgtgaga ccagtgtatc
      421 gcacagccgc gtgcacgcct cttctgcctg ctcgggcgca aaatggtcgg ctataaggcc
      481 cgacgcacgc gctgtgtccg cgtcaaagat ctcggcactc aggaaataac gtttggcaaa
      541 agccgtgccc gtcttgcgca gcacgtaggt cgagatgatc gccggaataa tacccagccg
      601 tacctcagaa aagcagaaac gggcgtcgct tgtggctaac gcaatatcgc aggccgcgag
      661 gattcccagg ccgccgccga atatgttacc ctgcgcaagc gcaaccgtag gctgcggcaa
      721 cccgtagatc atctcgaaca tgtgcgctaa gcgacgcgca tcttcgcggt tctcgtcgtc
      781 tgtgaaactg gcctgctggc tcatgtagtt gatatcagcc ccggcacaga acgacttgcc
      841 gttaccacgg aagagcacca gccgtgtctc tgccggcaca tcgtgaactg cctgcgtgac
      901 gcgcccgatc atttcagcat caaaggcatt gtgcttttcc gggcggttca ggataatttc
      961 gtaaacttgt tcgcggtagc gtgtttcaac catatgagtt ccctctttgc ttcttcagtc
     1021 aactacatgc gatagacgcc aggggtaaat gcctgcaacg ggcgccccgc cgcagcgagc
     1081 agacccataa tgagtgcacg gcgtgtttca cgcggatcaa taacaccgtc gtcccaaagc
     1141 cgggccgaag aataactcgc cgtagactgg ccgtcaaatt gcgcggtgat cttctggcgg
     1201 atctcatcga gctcacggcc gagccccgct tcgtcatgca acttgccaga tttctttgcc
     1261 tgcgcccggg cagactgctc gcgcacgatg gcgagcaccc cggcagcctg ctctcccccc
     1321 atgaccgaga tacgcgcatt gggccacatc agcagcaggt caggtgaata agcgcgaccg
     1381 cacattccat agttgcccgc gccatacgag ccaccgataa ttacggtaaa cttcgggacg
     1441 ttggcgttcg agaccgcgct caccattttt gccccatcct tggcgattcc accctcttcg
     1501 tactttttgc ccaccatgaa gccagtaatg ttttgcagga agatcagcgg aatttcgcgc
     1561 tggcagcaga gctcgataaa atgcgtaccc ttaagcgcag attcagagaa cagcaccccg
     1621 ttattcgcga gaatgccgac ccggaagccg ccaatttcgg caaatgcaca cacaagagtt
     1681 ttgccgtact ccgccttaaa ttcctgcagg ctgctgccat cgacgatgcg ggcgatgatc
     1741 tcgcgcacat cgaacggttt gcgggtatcg gcaggaatga tgccgggaat ctcttccacg
     1801 gacaggacgg gttccgctaa agaggtacgc cggccctgtg ccagcgcatt accctcagga
     1861 cgcggcaggg tcgcaacgat ttcacgtgca atgcgcagcg catcaaaatc atcgagtgca
     1921 tagtggtcgg caacgcccga gcggctcgtg tgtaaccgcg caccacccag gtcttctgca
     1981 ctgacctctt caccggtagc catcttcacc agcggcggcc cggccaaaaa gatcgtcccg
     2041 tgcttttcta cgataatggt ttcgtcgcac atggcgggta cataggcgcc accagctgta
     2101 cacgagccca tgacagcagc aatctgcgga atgccagcag ccgacatgcg cgcctggttg
     2161 aagaaaatac ggccaaagtg atcgcgatcg ggaaacactt catcctgcat gggcaagaat
     2221 gctcccccgc tatcgaccat gtagatacag ggcagccgat tgagctgcgc gaattcctgc
     2281 aggcgcaggt gtttcttcac tgtaatggga taatacgtgc cgcccttgac cgtggcatca
     2341 ttggccaata ccgcaaccat acggccatgc acaaaaccga tgccagcaac agctccagcg
     2401 cccgggatct cttcgcctgg gtagagatca agccccgcaa aagcgccgat ttcgaagaat
     2461 ggcgaaccag cgtccagcag cccggtaata cggtcgcgcg caaaaagttt gccgcgctct
     2521 gtgtgcttcg cgacggcagc ctgccgtgat tccggtgcaa tccgcttgcg cagcgcggcg
     2581 aggctatcca gatggcgccc gtacgcggca gcattttctt tgaattcagg gctctgcgaa
     2641 gagacttttg tttcgaatgt aggcatgatc ggctatttcc tgttggaacg catcctgaga
     2701 gtgtatcccg ctaacaacca ttttggcata acaaagacga aataaggcgt cagcaaagca
     2761 tgtgcctcgc aagggggagc tcccccgttc taagagcggg cagcgatctt gtttttctcg
     2821 gccgtatcgg acgacaacga gatgtcgagg ctgaactcgc ggggtttgga tacaatgagc
     2881 ggtacccgct cgacctcgac aatcgacgtg tcgagaccga aggctttttc ttcacccggg
     2941 cacatcgctt tccagagctt gtgcatcgcc gtcacaattt tttcgtagaa gttcagctcg
     3001 ttatcatgcg tcagtacgcg gtcgagcacg acgaaccgaa aatcgccgat gatatttttc
     3061 tcatgcagcg aagaatagcg actcgtgatg tcgacgatac cgcgctgcac caattcggtc
     3121 acgaccttgc ggaagtactg gttgatacgc ggttcgacgc gaaaacccag cttaaaatcg
     3181 acccttatga cgtcattggg taccagcacg tcgacgacac attcgcgcgt gtagggttca
     3241 tcggtcacgt cgacgtggac aaaccagtag atatctgcgc gtttcggttg cttgtagagc
     3301 agagaataga tgaccttcca ctcgatttcg tacttgttgt tcgccgaggt catatagaca
     3361 aggttcgtgc tgtacttgct caccgaagag tcgtggctca ggttgtgcag cgtatcgtag
     3421 tattcttcga gcctgacgaa ctcggtatac cggttgcgta tcttacgcgc ggaataccac
     3481 gaccagataa cagtgaatat gccgaaaccc atgacacccg ttacccatcc gccgtgtgaa
     3541 aattttccga gattcgcaac aaggaacgag gcttcgatgg cgagaaaact caccagggtg
     3601 acaactgcca ccagcatgct actgctgcga gagtagagaa aaacagacag taacatggta
     3661 gtcatgatca tgtcgagggt aatcgccagt ccgtaggctg cttccatgtt ggccgcctta
     3721 ccgaagaaca gcgtgacgcc aatacagcca acccagagca gccggttggc gctgggtacg
     3781 tagatctggc ctttcagaat cgaaggatac ttgatacgaa ccttgggcca gagattcagg
     3841 cgtatcgcct cagcgatcag ggtaaaggcg ccactgataa tcgcctgcga tgctatgatt
     3901 gccgcagcgg ttgcaatgag aattcccacg ggcagaaacc a
//
LOCUS       DAOQJF010000313         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14237_length_3913_cov_4.583981, whole genome shotgun sequence.
ACCESSION   DAOQJF010000313 DAOQJF010000000
VERSION     DAOQJF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14237_length_3913_cov_4.583981"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1675
                     /locus_tag="PLF85_14030"
     CDS             167..1675
                     /locus_tag="PLF85_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNE20837.1"
                     /translation="MNKKKFLLAALGAATLIVFPATTHAVDTSDSDFVPLSEMKPLEK
                     AEGYRNKHGYQFGVTAANTLINKKGVWVEDKPTYISDQSQGLFIGNYRPFFRYIKDEQ
                     HTFNVRGRFEYRYNPSLPDDPKLRGGEVVSNGTYNLEMLNAELDFDKHKVTVGRAFYR
                     MGRGLLFANFADGAEYTGNFRFFQVKALAAYSGEYGGCTISISGCRTNADSDIKAKAT
                     FDIIPGRPIDANVPDPGRRFFVGAEIQSAQLYGSNIYLLGLYSRDMTRDASTAGTNAG
                     KIFAFDPFYVGAGLQGYIATPRLRYLAEGIAQRGQTYAASTGSSNRQIDINAWALTAD
                     LNYSLPVVEKLLKPGLSLQYAYATGRDSTTPNPANPAQEATGATDNNFYAFGVYSAGL
                     ALQPRLNNLHVIRLGAQFRPLHYYYWGRNLMVSAKYTIYRKANADYGISDNTAAEKKA
                     GVGSGIDLQAVWDLRSDLKIFYAYGYFSPGEAYLSKDAKSFHSHIISINALF"
     gene            1679..3304
                     /locus_tag="PLF85_14035"
     CDS             1679..3304
                     /locus_tag="PLF85_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE20838.1"
                     /translation="MNSGDETTGSAEVYDTIFIGSGIGTILCASLLAQFRNERVLILE
                     KHFTPGGFTHTFKRKNRFFWDVGIHYIGDLQEGSILRKLFDLLSGQALRWQKMPAVFE
                     KFVYPDLEGKGEGYFTFPVYDDPDRFVADLARLFPAEEAAIRKYIDDIRKVNTWFGRH
                     VSIRGRAPFTDHLDEAFAQKEGFNPRHSVGEYIDARISDKRLKALLLSQWGNYGLPPA
                     QASFIIHAAVAQHYFHGGYYPLGGSHRIFESIVPFLEKTGSSVKTSHEVTEILVENGR
                     AIGVKAQALRGSEKSEQVFYGKKIISDAGAYNTYLRLLKKEPVPFAEKLETFYRKHPV
                     VSNVTLYLGLNGDPRQLGFGGENHWIYSSYDHDANFREGDTWIQKGRAAGAYLSFPSL
                     KNAEAKDHTAEVIAFTDYAFFERWKHQPWRKRDEEYRRLKDSITESLLQFIEARYPGF
                     RALVEYSELSTPITTEHFASHPRGSIYGVACTPERFVAHEAPWCNPVSPFENLYLTGA
                     DVSSPGVAGAMMGAVATLSQLPGGFKLLDLMRL"
     gene            3407..>3913
                     /locus_tag="PLF85_14040"
     CDS             3407..>3913
                     /locus_tag="PLF85_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE20839.1"
                     /translation="MPIDPKKYEETDEQVLWEEYKRAREPKLREYFIKKYAPLVKYVA
                     GKVAVGMPHNVEFDDLVSYGTFGLLDAIEKYDPSKEVKFKTYAMTRVRGGIFDELRNV
                     DWIPRSIRQKAKQVEKIILELENRMGRTAEDEEIAHELDIDLDEFHDLMSKISGTSLI
                     SLNDVWHGN"
BASE COUNT          960 a         1070 c          996 g          887 t
ORIGIN      
        1 gcaagcgctg gcgaagctga aatggcgttt tttgccggtg attttctttt ctccaataac
       61 ccttcggact ttgcagactt tatcggtttt cttgaatttc tcgacttcct gaattctttc
      121 gggacagtaa ctttcgaaaa actctgattc attaaaagca ttacctatga ataaaaagaa
      181 attcctgctg gcggcgctgg gcgccgccac ccttattgta tttcctgcga ccacacacgc
      241 cgtggacacc agcgacagcg actttgtccc cctgagcgaa atgaaaccgc tcgaaaaggc
      301 agaaggctac cgcaacaaac atggctacca gtttggtgtg acagcagcca ataccctgat
      361 caataagaaa ggtgtctggg tagaagataa acccacctac atcagcgacc aaagccaggg
      421 tctctttata ggtaactacc gtccgttttt tcgctatata aaggatgaac agcacacatt
      481 caatgtacgc ggtcgctttg aataccgcta caatcccagc ctgcccgatg acccaaagct
      541 gcgcggcggc gaagtcgtca gcaacggtac ctacaacctc gagatgctca acgccgaact
      601 ggattttgac aagcacaagg tgactgttgg acgcgcattc taccgtatgg gccggggact
      661 gctcttcgcc aactttgccg acggcgcgga gtacacggga aactttcggt ttttccaggt
      721 gaaggcgctg gcagcctatt ccggtgaata tggcggctgc accatttcta tctcaggctg
      781 ccggacaaat gcagattcag acatcaaagc aaaggcaact tttgatatta tacccggacg
      841 gcccattgac gccaacgtgc cagacccggg caggcgcttc ttcgtcggag cggaaatcca
      901 gtcggcgcaa ctctatggtt ccaatatcta cctgttgggt ctttactcac gcgacatgac
      961 ccgcgacgca tcgactgctg gcaccaacgc cggcaagatt ttcgcctttg atccatttta
     1021 tgtcggggct ggtttgcaag gctacattgc gaccccgagg ttgcgttatc ttgctgaagg
     1081 tattgcacag cgtgggcaaa cgtatgcggc aagcaccggc agcagcaacc gccagattga
     1141 tatcaatgcc tgggccttaa cggcagatct aaactactcg cttcccgtag tagagaaatt
     1201 gctcaagccc ggtctttcgc tgcagtatgc ttatgcgaca ggccgtgatt caacaacgcc
     1261 taatccggca aatcccgcac aggaggctac cggcgcaacc gacaacaact tctatgcctt
     1321 cggcgtctac tcagccggtc ttgctctgca gccacgcctg aacaacctgc acgtgatacg
     1381 ccttggcgcg cagttcaggc ccctgcacta ctattattgg ggtcgcaacc tgatggtctc
     1441 ggcaaagtat accatctacc gcaaggcgaa tgcagattac ggcatttccg acaataccgc
     1501 ggctgaaaaa aaagctggcg tcggcagcgg tatagacctg caggccgtct gggatctccg
     1561 cagtgacctg aagatttttt atgcgtatgg ttatttttct cccggcgaag cttacctttc
     1621 taaggacgca aaaagtttcc attcgcacat tatcagtatc aacgcgctct tctaaaatat
     1681 gaacagcgga gacgagacaa ccggctccgc tgaagtctac gataccatat tcattggctc
     1741 gggtatcggg acgattctgt gtgcctcttt gctggcacag tttcgtaacg agcgggtgct
     1801 gattctcgaa aagcacttca ccccgggtgg cttcactcac acattcaaac gcaagaaccg
     1861 cttcttctgg gacgtgggca ttcactatat cggcgacctg caggaaggtt cgatcttgcg
     1921 caagctgttc gacctgctct cgggtcaagc actgcgctgg caaaagatgc cagccgtttt
     1981 tgagaagttt gtctacccag acctcgaagg aaaaggtgag ggatatttca cttttcccgt
     2041 ctatgacgat cccgatcggt tcgtggcaga cctggcaaga cttttccccg ctgaagaagc
     2101 ggcgatacgc aaatacatcg atgacatccg caaggtaaat acctggtttg gccgccacgt
     2161 ttcgatacgc ggccgtgcgc cgtttaccga ccatctcgac gaagcttttg cacagaaaga
     2221 aggtttcaac ccgcgtcaca gcgtcggtga atacattgac gcgcgcatca gcgacaagag
     2281 acttaaggca ctgctgcttt cgcaatgggg caactacggt ctgccgcccg cacaggcgtc
     2341 attcatcatt cacgccgcag tggcacagca ttactttcac ggcggttact accccttggg
     2401 cggttcgcac cgcatcttcg aaagcattgt tccttttctc gaaaaaaccg gcagcagtgt
     2461 caaaacctcc cacgaagtca cggagatact ggttgaaaac gggcgtgcca ttggtgtaaa
     2521 ggcgcaggcg ctgcggggga gcgaaaaatc agaacaggta ttttacggta aaaaaattat
     2581 ctccgatgcc ggtgcgtaca atacatacct ccgcctctta aaaaaagaac ccgtaccctt
     2641 tgcagagaag ctggaaactt tttatcgtaa acatcccgtt gtgagcaatg tcacgctgta
     2701 cctgggtctg aacggcgatc cgcggcagct gggcttcggg ggcgaaaacc actggatcta
     2761 ttcatcatac gaccacgatg ccaactttcg cgaaggtgat acctggatac agaaaggcag
     2821 agcggcgggt gcatacctgt cgtttccttc gttgaagaat gccgaagcca aagaccatac
     2881 tgccgaggtg attgcattca ccgattacgc attttttgag cgctggaaac atcagccctg
     2941 gcgtaagcgc gatgaagagt accgccggct caaagattcg atcaccgaat ctctgctgca
     3001 gttcattgag gcccggtatc cgggctttcg agcactcgtc gaatacagcg aactttcgac
     3061 accgattacc accgaacact tcgccagtca cccgcgcggc agtatctacg gtgtcgcctg
     3121 tactccggaa aggtttgttg cccatgaagc accgtggtgc aacccggtat cgccgtttga
     3181 gaacctctac ctgacagggg ccgatgtttc gtcgcctggg gttgcgggag caatgatggg
     3241 agctgtcgct accctgtcgc agcttccagg cggcttcaag ttgctcgacc tgatgaggtt
     3301 ataaacctat gtgcgatgca cataggtcat tatccggcgc gagatctgct cattcctgag
     3361 cgaaaacact tgacgcacct gtggccggcg aagcacgggt gacataatgc ccattgatcc
     3421 caagaaatat gaagaaaccg atgagcaagt tctttgggaa gagtacaaaa gggcccggga
     3481 gccgaagctc cgcgagtatt tcatcaaaaa atatgccccg ctcgtcaaat atgtagccgg
     3541 taaggtagcg gtcggcatgc cgcacaacgt ggaattcgat gacctcgtaa gttatggcac
     3601 ctttggcctg ctcgacgcga tcgagaaata cgacccttca aaagaagtta aattcaagac
     3661 ctatgccatg acacgcgtgc gtggtggtat tttcgatgaa ctgcggaatg tcgactggat
     3721 tccgcgctcc atacgccaaa aggcaaagca ggtcgagaag atcatcctcg agctcgaaaa
     3781 ccgcatgggc cgcacggctg aggacgaaga gattgcgcac gagctcgata tcgacctcga
     3841 cgagttccac gacctgatgt cgaagatctc cggcacctcc ctgatctcgc ttaacgacgt
     3901 ctggcatggc aat
//
LOCUS       DAOQJF010000314         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14296_length_3905_cov_5.013506, whole genome shotgun sequence.
ACCESSION   DAOQJF010000314 DAOQJF010000000
VERSION     DAOQJF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14296_length_3905_cov_5.013506"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2048
                     /locus_tag="PLF85_14045"
     CDS             <1..2048
                     /locus_tag="PLF85_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE20840.1"
                     /translation="MWQTILRGETWRGEICNRRKDGSFYWVKTVITPLRDSEGEIESF
                     LAIRQDITELKETQQDLASIQRQYASLVENATDIIARYDRNLRITFINQAVTKLFFEP
                     SEYFVGKTNAELDLPPHVIANWENQLRKVMESGKPSVIESAIDFAGRKIYFQTSAVPE
                     FDAQGAVESLLLSTRDVTSQKQAEIVLQQRDERLQGIIDGLPGMVGYWDTNLRNRFAN
                     TSYIEWFGKLPHEIHGIHIRELLGDDLFELNRPFFERALQGERQIFERAIPRPDGSTR
                     WTQAYYIPDNQAGSIVGFFVFVFDISAIKEAEVRAKEANEAKSRFLSNVSHELRTPLN
                     GIMGFAQLVSISSDAPASVREYGGHIEASAEHLLALINELLDFGRIEAGAVQIEPEQI
                     TLERALRTVMELITVQARAKGLEFLSEFEATPSLVLHVDVKRLRQVLLNLLTNAVKFT
                     QQGTIRLRIAVHDGSLRFEVSDQGVGIEAVDLQRLFVPFSQVGKRAFRSQGLGLGLAI
                     SQQLVQAMGGEITVTSKPGQGSTFAFTLPCINPAAAPSTGLRPTQSARGKLRLTALYV
                     EDDYRGRQLMQEFLRNSQIDLLLAEDAASALRIAEQSEPDLFLLDLNLPDMSGFDLLA
                     KIRATAWGSNKPVIAISADHMQPTRDRALESGVAEFVPKPINLARLNAVLSRYAPHG"
     gene            2113..2481
                     /locus_tag="PLF85_14050"
     CDS             2113..2481
                     /locus_tag="PLF85_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20841.1"
                     /translation="MSYELRWHPGGVTKYFFAKVTNEDMLLSVYESEADPRFENLRYV
                     INDFLDITGCDFEAPIAERIAIQDRGAAFTNPNIRIAVVTTHPQVIAAAEAYSASPMN
                     IYQTRIFPTMTEARQWLSVD"
     gene            complement(2568..>3905)
                     /locus_tag="PLF85_14055"
     CDS             complement(2568..>3905)
                     /locus_tag="PLF85_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNE20842.1"
                     /translation="AYAGHQFGHFTMLGDGRAILLGEQVTPAGQRFDLQFKGSGQTPF
                     SRRGDGRAALAPMLREYIISEAMFALGIPTTRSLAVVATGEPVYRENVLPGAMLTRVA
                     ASHIRVGTFEYAASIGADNRALQFDRLTNRAVEGQGGRSPLEALADYTIARHYPEIGP
                     REDRYLAFFDAVAERQAQLIARWQCTGFIHGVMNTDNMALSGETIDYGPCAFMDAFDP
                     ATVFSSIDQHGRYAFGNQPRIAAWNLARFAETLLALFADDEQQAIAIGQQALARFNER
                     FNHHYSLGMQAKLGLVNHEEADVELVTELLTLMQQHAADYTNTFRRLSGRAAASAENH
                     GTEANALPGTEALFAAAEFTAWAGRWWARIKQQSISPAEALTNMRAVNPAVIPRNHKV
                     EEALGAAVSAGDLSVMESLLAALKNPFVETEANLAYRSPAPETGEPYRTFCGT"
BASE COUNT          839 a         1071 c         1123 g          872 t
ORIGIN      
        1 atatgtggca gacgattctc cgcggtgaaa cctggcgcgg tgaaatttgc aaccgccgca
       61 aagatggctc tttctattgg gtcaagaccg ttattacccc attgcgggat tcggagggtg
      121 aaatcgaatc cttcctggcg attcgccagg atatcaccga actcaaagag acacagcaag
      181 atcttgccag tatccagcgg cagtacgcca gcctcgtgga aaacgcaacc gatatcatcg
      241 cgcgctacga ccggaacctg cgaatcacat tcattaacca ggcggtaacg aagctatttt
      301 ttgagccaag tgaatacttc gtcggcaaga cgaatgcaga gctcgacttg ccgccgcacg
      361 taatcgccaa ctgggaaaat cagttacgca aagttatgga atcgggcaag ccttcagtca
      421 tagagagtgc cattgatttt gcagggcgaa aaatatactt ccagacgagc gccgttcctg
      481 agtttgatgc gcaaggcgca gtggaaagcc tgctcttgag tacgcgcgac gtcactagcc
      541 agaaacaggc agagattgta ttacaacagc gcgacgagcg gctgcagggt attatcgacg
      601 gtttgcccgg catggttggt tactgggata caaacctgcg caaccgcttt gccaacacaa
      661 gctacatcga atggtttggc aaactgccgc atgaaattca cggtattcac atacgcgaac
      721 tgcttggtga tgacttgttt gaactcaacc ggcctttttt tgaaagggcg ctgcagggcg
      781 agagacagat ttttgaacgc gcaattccaa gacccgatgg atcgacgcga tggacgcagg
      841 cctattacat ccctgataat caggcaggat ctatcgttgg tttttttgtt ttcgtctttg
      901 atatctctgc cattaaagag gctgaggttc gcgccaaaga ggccaacgaa gcgaagagtc
      961 gcttcctctc gaacgtgagc catgagttac gcactccgct caatggtatc atgggttttg
     1021 cgcagctggt ctcgatcagc agcgatgcgc cagccagtgt gcgcgagtac ggcggtcata
     1081 tcgaagcaag cgccgagcac cttttggcgc tcattaacga attgctcgac tttggtcgta
     1141 ttgaagccgg ggcggtacaa attgaacccg agcaaatcac gctcgaaagg gcgttgcgca
     1201 ccgtgatgga attaattacc gttcaggcgc gcgctaaagg tctggaattc ctgagcgaat
     1261 tcgaagcaac accgtcgctg gtactgcacg tagatgtaaa gcgcctgcgg caggttctgt
     1321 tgaacctgct gacgaacgcc gtgaagttta cgcagcaggg taccattcgg ttgcggatag
     1381 ccgtgcacga tggctcactg cgttttgagg tgagcgacca gggggttggc attgaagctg
     1441 tcgacctgca gcgcctcttt gtaccttttt cccaggtggg taaaagggca ttccgcagcc
     1501 aggggctggg gttaggtctc gccatcagcc agcaattggt gcaggcgatg ggcggagaga
     1561 taacggttac gagtaagcca ggccagggca gcacatttgc gtttacactg ccctgtatta
     1621 accctgcggc agctccttcc accggtttac ggccaacgca gtcggccagg ggtaagctcc
     1681 gccttacagc actctacgtc gaagacgatt accgcggccg ccagctaatg caggagtttc
     1741 tgcgcaactc gcagattgac ctgctgctgg cagaagatgc cgcatctgcg ctcagaatcg
     1801 ctgagcagtc agagcccgac ctgttcttgc tcgatctgaa tttacccgac atgtcgggtt
     1861 ttgacctgct ggcaaaaatt cgggcgactg cctggggcag caacaaaccg gtgattgcga
     1921 tctctgcaga tcacatgcag ccgacacgcg accgcgcgct tgaatcgggc gtagcggaat
     1981 tcgtgccgaa acctattaac ctggcacgcc tgaacgccgt actcagccgc tacgcaccgc
     2041 acggttagta ctggaaccga tcaggggctt aactgcttgc catcgtaatc gactggcaaa
     2101 ctctgaagat gcatgagcta cgaattgcgg tggcatccgg gtggcgtaac caagtatttc
     2161 ttcgctaaag tgaccaacga agatatgcta ctttcggttt atgagtcgga agccgatcca
     2221 cgctttgaga acctgcgcta cgtcatcaac gacttccttg atattaccgg ctgtgatttt
     2281 gaagcaccca tagcagaacg cattgctata caggatcgcg gtgcggcgtt cacaaacccg
     2341 aatattcgta ttgcggtagt cacaactcat ccgcaggtga ttgccgccgc agaggcgtat
     2401 tctgcttcac cgatgaatat ctaccaaacg cgtatttttc caacgatgac tgaagcgcgg
     2461 cagtggcttt cagttgattg aattttccct gccataaatt gcattgatca gaatctctta
     2521 attagtttca ccggcgcacc gaacctaacg gttaaaacgt tgcttcctca cgttccgcaa
     2581 aacgtgcggt acggttcgcc cgtttcgggt gcgggtgagc ggtaagcgag gttcgcttct
     2641 gtctcgacaa atggattttt gagcgctgcg agtagcgact ccatgaccga aagatcacca
     2701 gcgctcacgg cagcgccgag cgcctcttca accttgtggt tgcgcggtat gactgccgga
     2761 ttgactgcgc gcatgttcgt gagtgcttca gccggtgata tgctctgctg ttttatgcgc
     2821 gcccaccatc tgccagccca cgcggtgaat tctgccgcgg cgaagagtgc ctcagtgccg
     2881 ggtagggcgt tggcttcggt gccatgattt tcggcagatg ccgctgctct gccgctcaaa
     2941 cgtcgaaacg tattcgtata gtccgcggcg tgttgctgca tcagtgttaa gagttctgtc
     3001 accagttcta catccgcctc ttcatggttc acgaggccca gcttcgcctg catgccgagc
     3061 gagtagtggt ggttaaagcg ttcgttgaag cgcgcgagtg cctgctggcc gatcgcaatc
     3121 gcctgctgtt catcgtcggc aaaaagtgcg agcagcgtct cggcaaagcg cgcgaggttc
     3181 cacgcggcga tgcgcggctg gttgccgaac gcgtagcggc cgtgctggtc gatcgagcta
     3241 aaaaccgtcg cggggtcgaa cgcgtccatg aacgcgcacg ggccgtagtc gatcgtctcg
     3301 cccgaaagcg ccatgttgtc ggtgttcatc acgccgtgta tgaacccggt gcactgccag
     3361 cgcgcgatca gttgcgcctg ccgctcggcg accgcgtcga agaaagcgag gtagcggtct
     3421 tcacgcgggc caatttcagg gtagtgtcgc gcgatggtat agtcggccag cgcctctagt
     3481 ggggatcgac cgccttgccc ttcgactgcg cggttggtga gcctgtcgaa ctgcagggcg
     3541 cggttatcgg cgccgatgct tgccgcgtat tcgaaagttc ccacgcgaat atggctcgcc
     3601 gcgacgcgcg tcagcattgc gcccggcaaa acattttcgc ggtacactgg ctcacctgtc
     3661 gccaccaccg cgaggctgcg tgtcgtgggt atgccgagcg caaacatcgc ctcgctgatg
     3721 atgtactcgc gcagcatcgg ggcgagcgcc gccctgccgt ccccgcgccg tgagaagggc
     3781 gtctgtcccg aacctttgaa ctgcagatcg aatcgttggc ccgcgggtgt gacctgttcg
     3841 ccgagcagaa tcgcgcgccc gtcgccgagc atggtgaaat gtccaaactg gtggccagcg
     3901 taggc
//
LOCUS       DAOQJF010000315         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14414_length_3887_cov_4.240866, whole genome shotgun sequence.
ACCESSION   DAOQJF010000315 DAOQJF010000000
VERSION     DAOQJF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14414_length_3887_cov_4.240866"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1495
                     /locus_tag="PLF85_14060"
     CDS             113..1495
                     /locus_tag="PLF85_14060"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNE20843.1"
                     /translation="MQREITRSFIEKEIRPLSQRYAFARALLIETSGLTVSSNLNQTF
                     VEPLSAQRGIVVSILHNGLLYEGSSVVHSARDVSRTIAELEDTVRQSRHQGTVKLIPE
                     EPMEFTAGNPPADDLTENDKATFATRMAREIKARNPLVAMASARYKQVNTREIYVSGS
                     KSLEQYLPRFEAIYSAVLKDNATQSTAQIYDGFSRQGSWHLMADNLELIDGMLHDGQR
                     ILGAPRLTPGYYDCVFSPSLSGMLAHEAFGHGTEADTMQKGRARGTEYLGKMVASDKV
                     RLYDSPQLEGAAASYHFDHEGMPAAQTQIVEQGVLTQPMTDHASASRMGYRRSPNGRR
                     ESFDHKIYSRMTNTYFMPGNDDLQSMIASITDGYFVDRATNGMEDPKSWGIQLEALVA
                     RRIRNGRLTDEYFSPVIVTGYVPEILQSISMVSGDLHINGLGMCGKGYKEWVKVTDGG
                     PYLKLRARLA"
     gene            complement(1475..2800)
                     /locus_tag="PLF85_14065"
     CDS             complement(1475..2800)
                     /locus_tag="PLF85_14065"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE20844.1"
                     /translation="MADDQVQAEEAETTGKPGEEAGGEGKPAFVFESFRDFLNFVSYV
                     GEGVQVEFAEPVLDGTGTALVQKGVHMRPSLQKSLQQFYDRGNLNEKITIADTQALHT
                     AIRDRVVRSLTRCLEPGRFHMAQALTDISTINIRSMLVSIIQRRDVLPVFMRLDQSED
                     PLLPHLGEVALVAGGMAEQYCRNAGKNAGAREVIRTAIFAGLLHDIALAGDDDFLLKD
                     IEKITESEHARKSAEQAKALIPALPVSVTDIIEHHHRMNNPYEPESDSTLEAQSIAAE
                     ALALSEYIFVQLRSQYKKDETMNSAELLFYELGRAFGQGKFHPQFRRIAARVWETLFA
                     TLHYGYEIGAVESQCPHKPSAIAYPTPRCTQVMCHRHVTGCEHYDTQFPLEILQATRF
                     PGRPGALIEPGKYGKCRLATKLPKGINEKLSADAWLKQTSAAKSGEAGA"
     gene            complement(2834..>3887)
                     /gene="rlmN"
                     /locus_tag="PLF85_14070"
     CDS             complement(2834..>3887)
                     /gene="rlmN"
                     /locus_tag="PLF85_14070"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNE20845.1"
                     /translation="QGKTPLANETKPEILGLTEDELAAYLVADGHKKFRAQQVFQWLH
                     LKQVFDVRQMSNLKAEFQDYLTEKFIFPEFRILNETKSPSDQTAKYLFEFGNKQVESV
                     WLPYENRQSICISVQSGCSLDCSFCATGKIAFKGNLTTGQILSQVYGMQQLYDRRITN
                     VVFMGMGEPFYNYDNAIKAAGLLAHQHGMNISRRRITLSTSGVLPGIKRFIEEKQPYR
                     LALSLHAVFPEKRRAIMDIEEKFAFEEVLEYLGEHRAEMQQGQLMFEYIMIDGINISP
                     EDAKELARWAKLLRAKVNLIPMNTSFNGMHRPTDEKIYDFWRQLVERDVVAVNRKSPA
                     KDIDGACGMLAGKKAS"
BASE COUNT          798 a         1081 c         1140 g          868 t
ORIGIN      
        1 acaaccactc ggagctcgac aagggttcta tcggaaaaaa gaacaacctt tcgcccgcgc
       61 aaatgaagaa gatgcaggat tacgtcgaca agaagtttgc cgccctgcgc taatgcaacg
      121 cgaaattacc cgcagtttta ttgaaaaaga gatacggccg ctgtcgcagc gttatgcatt
      181 cgcacgcgcg ctgttaatcg agacctctgg actcactgtc agcagtaacc tgaaccagac
      241 ctttgttgaa ccgctgagcg cgcagcgggg tatcgtggta agtattctgc ataacggtct
      301 gctttacgaa ggttcgtccg tcgtgcacag cgcgcgtgat gtcagccgta ctatcgccga
      361 gctcgaagac acggtgcggc aaagccggca ccagggtacg gtaaagctca tacctgaaga
      421 gcccatggag tttactgcag gaaatccgcc cgcggacgat ttgaccgaaa acgacaaagc
      481 aactttcgcc acgcgcatgg cacgcgagat caaggcgcgc aacccgctcg tggcaatggc
      541 ttcggcgcgc tacaagcagg tgaacacgcg cgagatctat gtgagcggca gtaagtcgct
      601 cgaacagtac ctgccgcgtt tcgaggcaat ctattcggcc gtgctgaaag ataacgcgac
      661 gcagtcgaca gcgcagatct atgacggttt cagccgccag gggtcatggc acctgatggc
      721 agacaacctc gagctgatcg acggcatgct gcacgacggg cagcgaattc tgggtgcgcc
      781 gcggcttacg ccgggttact atgactgtgt attttcgcca tcgctttcag gcatgctggc
      841 gcacgaggcc ttcggtcatg gcaccgaagc cgatacgatg cagaaagggc gcgcacgcgg
      901 caccgagtac ctcggcaaga tggtggcgag cgacaaggtc aggttatacg attcgccgca
      961 gctcgaaggt gccgccgcga gctaccattt tgatcacgag ggaatgcccg ctgcacagac
     1021 gcagatcgtc gaacagggtg tactgacaca gccaatgacc gaccatgcga gcgcatcgcg
     1081 tatgggctac cggcgttcgc cgaacggccg gcgcgaaagt tttgaccaca agatctattc
     1141 gcgcatgacg aatacgtatt tcatgccggg caacgatgac ctgcagagta tgatcgcctc
     1201 gattaccgac ggctatttcg tcgatcgtgc gaccaatggc atggaagacc ccaagagctg
     1261 ggggatacag ctcgaggcgc tggtcgcgcg ccgcatacgt aacggtcgtc tgaccgatga
     1321 atacttttca cccgtgattg tgacgggtta tgtgccagag attctgcagt cgatctcgat
     1381 ggtatccggc gacctgcaca tcaacggcct cggcatgtgc ggtaagggtt ataaagaatg
     1441 ggtcaaggtt accgatggcg gcccgtacct gaagttacgc gcccgcctcg cctgacttag
     1501 ccgccgatgt ctgtttcagc catgcatcgg cgctcagctt ctcgttgatg ccttttggca
     1561 gttttgtcgc cagccggcac ttgccatact tgcccggttc gatcaatgca cccggtctgc
     1621 cgggaaagcg cgtcgcctgc agtatttcaa gcggaaactg ggtatcgtag tgctcgcagc
     1681 cggtaacgtg acggtggcac attacctggg tacagcgcgg cgtcgggtag gcgatggccg
     1741 agggtttgtg cgggcactgg ctttcgacgg cgccaatctc gtaaccgtag tgcagcgtcg
     1801 caaaaagtgt ctcccagacg cgggctgcga tgcgcctgaa ctgcgggtga aattttccct
     1861 ggccgaaagc gcgcccgagt tcgtagaaca ataattctgc cgagttcatc gtctcgtctt
     1921 ttttatattg ggatctcaac tgcacgaaaa tatattcgct gagcgcaagg gcttctgccg
     1981 caatagactg cgcttcgagc gtagaatcgc tctcgggctc gtacggattg ttcatcctgt
     2041 ggtggtgttc aatgatgtca gtcacactca ccggcagcgc gggtattaat gcctttgcct
     2101 gttcggcgct cttgcgcgcg tgttcgctct cggtaatctt ttcaatatct ttcagcagaa
     2161 agtcgtcgtc accggcaagc gctatgtcgt gcaacagacc ggcaaatata gctgtgcgga
     2221 ttacctcgcg cgcgccggcg ttcttgcctg cgttacggca gtattgctcg gccattccgc
     2281 cggcaacgag cgccacttcg cccaggtgcg gcagcagcgg atcttcggac tggtcgaggc
     2341 gcatgaagac tggcagcaca tcgcgccgct ggataatgct gacgagcatc gaccggatgt
     2401 tgatcgtcga aatgtcggtc agcgcctgcg ccatgtgaaa gcgcccgggt tcaagacagc
     2461 gggtcagcga gcgcacgacg cggtcgcgta tcgctgtgtg cagcgcctgg gtgtcggcga
     2521 tggtgatttt ctcgttgagg ttgccgcggt cgtagaactg ctgcaggctc ttctgcaggc
     2581 tgggccgcat gtgcacacct ttttgcacga gcgccgtgcc ggtgccgtcg agtacaggtt
     2641 cggcgaattc gacctgtaca ccttcgccaa cataactcac gaaattcaga aaatcacgga
     2701 acgattcaaa gacgaaggct ggcttgcctt cgccaccggc ctcttctccg ggcttcccgg
     2761 tagtttccgc ttcttcggcc tgtacttggt cgtcagccat ggcgtgaggg caaatgccgg
     2821 cgcgagcgac ctgtcaggaa gcctttttac cggccagcat gccgcaggcg ccgtcgatat
     2881 ccttagcggg agacttgcgg ttcacggcaa ccacgtcgcg ttcgaccagc tgccgccaga
     2941 agtcatagat cttttcgtcc gtggggcggt gcatgccgtt gaatgaggta ttcatcggta
     3001 tcaggttcac cttggcccgc aagagctttg cccagcgcgc gagctccttc gcgtcttcgg
     3061 gggaaatatt gatgccgtcg atcatgatgt actcaaacat aagctggccc tgctgcatct
     3121 cggcgcggtg ttcgcccagg tactcgagca cttcttcgaa ggcgaatttc tcttcgatgt
     3181 ccatgattgc ccggcgcttt tcggggaaaa ctgcatgcag cgaaagcgcc agccggtatg
     3241 gctgcttttc ttcgatgaag cgtttgatgc cgggcaagac acccgaggta ctgagtgtaa
     3301 tgcgccggcg cgagatattc atgccgtgct ggtgcgcgag caggcccgct gctttgatgg
     3361 cattgtcgta gttatagaac ggttcaccca tgcccatgaa gaccacgttc gtgatgcgcc
     3421 ggtcgtacag ctgctgcatg ccatagacct gcgagagaat ttgcccggtc gtgagattgc
     3481 ccttgaaggc gatcttgccg gtagcgcaga agctgcagtc gagcgagcaa cccgactgca
     3541 ccgatatgca gatcgactgc cggttttcat aaggcagcca gacgctttca acctgtttgt
     3601 tgccgaattc gaacaggtac ttcgctgtct ggtcagaggg ggatttcgtc tcgttcaaaa
     3661 ttctgaattc agggaatatg aatttttccg tcaggtaatc ctggaactcg gctttcaggt
     3721 tgctcatctg ccgcacgtca aagacctgct tgaggtgcaa ccactggaag acctgctgcg
     3781 cgcgaaactt cttgtgcccg tcggcgacaa gatacgccgc aagttcgtct tcggttaatc
     3841 ctaaaatttc tggtttcgtt tcgtttgcca agggggtctt gccttgg
//
LOCUS       DAOQJF010000316         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14785_length_3826_cov_7.306285, whole genome shotgun sequence.
ACCESSION   DAOQJF010000316 DAOQJF010000000
VERSION     DAOQJF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14785_length_3826_cov_7.306285"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLF85_14075"
     CDS             complement(<1..462)
                     /locus_tag="PLF85_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20846.1"
                     /translation="MKKLFWVAALLLAISVSTLCGGQNETLATFDGGSVTTKDVEAEN
                     EQEFHEIRMKEFQVKQQSTFEKAREMIFAAEAKKRGITVEQLVETEMAKRRGNVGEEQ
                     IRQFYEYNKSRINQPFALVKDRIRQQLVQNNEKGARDSLTSELFRAYNFKFN"
     gene            757..1728
                     /locus_tag="PLF85_14080"
     CDS             757..1728
                     /locus_tag="PLF85_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE20847.1"
                     /translation="MPVIESNYRHPRLLKNPHLHTVLSAVLRKKPQVAYVRERLELPD
                     GDFIDLDWSVKDTRRLVILAHGMEGSSKSKYILHTVQAINNAGVSAVAWNMRGCSGDI
                     NRKLHFYHSGKTEDLAAVVDHARQLGFRKIYLVGFSLGGNLTLLYLARAAEKIRREIQ
                     RAVAICAPVDLVTSQDQIERKENRLYLKRFLRDFHKKFERKRQHRKIYIDTQKFHRRI
                     RTLAHLDAHYTAPWNGFPNEKEYYRSASSLPLLQQIRIPTLLLNPKDDTFLSENCYPV
                     AEAKNSNLFHLEMPETGGHCAMLTNYRLRESYMERRILEFLQLNPTG"
     gene            complement(1712..2785)
                     /locus_tag="PLF85_14085"
     CDS             complement(1712..2785)
                     /locus_tag="PLF85_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE20848.1"
                     /translation="MGGLAEMVRDVENVFARSLKAIPEADLIAYFSAVGTGEQKRAAV
                     LREYHAQRAAVARPLPLITPPPARRHTRAQRLNAGSLVAYLNNMRQVLRVRNYSQATL
                     KAYTRDMLAFADWLHGRQLALTPQVSERVAMEFMLARREQGCSNQSLRGFRAALKIFC
                     ESNGKTVDFRLIRTSRGDRALPVVLSTDEIGRLLSAIKNRKHWVMVSLMYSSGLRVSE
                     VVKLRVGNLDLAENTLIVERGKGRKDRLTIISKTQRDVLAELMRGRPGHAFLFESSHQ
                     RGRPIAVRSLQKVVERAIKAAGIKKAASSHSLRHSFATHLLEGGTDIRHIQKLLGHEH
                     IRTTATYTRVAKTTLRQIVSPLG"
     gene            complement(2891..3214)
                     /locus_tag="PLF85_14090"
     CDS             complement(2891..3214)
                     /locus_tag="PLF85_14090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016755754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNE20849.1"
                     /translation="MVVEQYDVFLINLDPTIGHEIKKTRPCLVISPNEMNGNISTIII
                     APMTTVSRNYPTRVKTVFKKKTGFIVLDQIRTVDKARLVKKLGRVNIDAITSVKAILR
                     EMLID"
     gene            complement(3208..3456)
                     /locus_tag="PLF85_14095"
     CDS             complement(3208..3456)
                     /locus_tag="PLF85_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNE20850.1"
                     /translation="MILRVVKVGNSKGLRIPKSILEQCHIEDEVDITPTKDGLLLKPV
                     KSKARAGWAKKFKEMATHRDDKLLIPDFTDTADRDWQW"
BASE COUNT          959 a         1044 c          889 g          934 t
ORIGIN      
        1 gttgaacttg aagttgtatg ccctgaatag ttctgaagtc aaagaatcac gcgcgccctt
       61 ttcattgttt tgtaccagct gctggcgaat acgatctttc accagggcaa acggctggtt
      121 gatgcgcgac ttgttgtatt cataaaactg gcgaatctgc tcttcgccga cgttgccccg
      181 acgcttcgcc atttctgttt ccacaagttg ttccacagtg atgccgcgct tttttgcttc
      241 ggcagcgaag atcatctcgc gcgctttttc aaacgtcgac tgttgcttga cctggaactc
      301 tttcatgcgg atctcatgga attcctgctc gttttctgct tcgacgtctt tcgtcgtcac
      361 cgaaccgcca tcgaaggtcg cgagcgtttc gttctggccc ccgcagagcg tagagaccga
      421 gattgccagc aagagtgcgg caacccaaaa tagtttcttc ataacatctc tgaatgtatt
      481 ttcactgtgc gtggaaagtc tttttagaat ttatgctaat tcaataagca taacacgaaa
      541 gagtcacggc aaagttggag taaacttacc cacagttgtc caccgccaaa accgcagaaa
      601 atccccttgt ggaaaaggag acataattag gcgacataat ccacacgcga attttaaaaa
      661 aacccttaaa attcgcgtgt ttttgcagtt ttccactttt ccacatgatt attagtatta
      721 taataactaa acacctatat acatatatat aatacaatgc ccgtcattga gtcaaactac
      781 cgccacccgc gcctgctgaa aaacccgcac ctgcataccg tgctgagtgc agtgctgcga
      841 aaaaaacctc aggttgctta cgtgcgcgaa cgccttgaac tacccgacgg cgatttcatc
      901 gacctcgact ggtctgtcaa agacacacgt cgcctcgtca tactggcgca cggcatggaa
      961 ggctcgagca aaagcaaata tatactgcat accgtgcagg cgatcaacaa tgcaggcgtc
     1021 tctgcagtcg cctggaacat gcgcggctgt tcgggcgaca tcaatcgcaa actgcacttc
     1081 taccacagcg gcaaaaccga agatctcgcc gcagtcgtcg accatgcgcg ccaattagga
     1141 tttcgtaaga tttacctggt gggtttcagt ttgggcggta acctcaccct gctgtatctc
     1201 gcacgcgcgg cagaaaaaat tcgccgcgaa atacagcgtg ctgtcgcgat ctgcgcaccc
     1261 gtcgacctcg tcacttcgca ggaccagatc gagcgcaaag aaaaccgtct ctacctcaaa
     1321 cgtttcttgc gcgacttcca caaaaaattc gagcgcaaac gccagcaccg caagatctat
     1381 attgatacac aaaaattcca ccgacgcata cgcacgctcg cgcacctcga cgcgcactac
     1441 accgcgccct ggaacggctt tccgaacgaa aaggagtact accgttctgc gagctcattg
     1501 ccactgctgc agcagatacg cataccgacg ctgctgctca atcctaaaga cgataccttc
     1561 ttgagtgaaa attgttaccc tgtcgccgaa gcgaagaact caaacctgtt tcatcttgaa
     1621 atgccggaaa ccggcggtca ctgcgcgatg ctgaccaatt accgcctgcg cgagtcgtac
     1681 atggaacggc gtatactgga atttttgcag cttaacccaa cgggctgacg atttgccgta
     1741 gcgtggtctt cgctacgcgc gtgtaagtcg ccgtagtgcg tatgtgctcg tggccgagca
     1801 gcttctggat atgccggatg tcggtaccac cctcaagcag gtgtgtggca aacgaatggc
     1861 gcaggctgtg gctgctggct gccttcttga tacctgcagc tttgatcgcg cgttcgacaa
     1921 ccttctgcaa tgaacgcacc gcaatcggtc gcccgcgctg gtgtgaagat tcaaataaaa
     1981 aagcatgacc cggtctgccg cgcatcaatt cagccaacac atcgcgctgc gttttggaaa
     2041 taatcgtcag ccggtctttt cggcctttac cgcgctcgac gatcagcgtg ttctcggcaa
     2101 gatccaggtt gcccacgcgc agcttgacga cctcggagac gcgcaggccc gacgaataca
     2161 tcagcgaaac catcacccag tgtttccgat ttttaatcgc cgataacagc ctgccaatct
     2221 catccgtaga cagcacgacc ggtaaagctc tatcgccgcg cgacgtgcga attaaccgaa
     2281 agtctacggt cttaccattg ctttcgcaaa aaattttcag agctgcacga aatcccctca
     2341 ggctttggtt gctgcaacct tgctcgcgcc gcgccagcat gaattccatt gccacacgct
     2401 cgctcacctg cggggtcagc gccagctgcc ggccatgcag ccaatcggca aacgccagca
     2461 tgtcgcgcgt atatgccttc agcgtcgcct gcgaatagtt gcgcacgcgc agcacctggc
     2521 gcatgttgtt caggtaagcg accaatgacc ccgcgttcag ccgctgcgcc cgtgtgtgcc
     2581 gcctggcagg tgggggagtg atgagcggta agggtctggc aaccgcagca cgctgcgcgt
     2641 ggtactcgcg cagaactgcc gcgcgctttt gctcaccggt acccacggca gaaaaatacg
     2701 caatcagatc agcctcagga atcgccttca gcgaacgcgc gaagacattt tccacatcgc
     2761 gcaccatttc agccagtccg cccataaata gaacataatt agaacagtta cagcgtaaag
     2821 tatttttcaa aaaaactaca aaccaagttg gattagcata caagttacag gtgaatgaaa
     2881 tttgcgcgga ctaatctatt agcatttccc gtagaatagc tttaactgac gtaatagcat
     2941 caatattaac cctaccaagc ttcttaacca ggcgcgcctt atcgacagtc cgaatctggt
     3001 cgaggacaat gaatccggtc ttctttttaa aaacggtttt cacgcgcgtc ggataattgc
     3061 gggaaactgt tgtcatgggg gcgattatta tcgttgaaat attaccgttc atttcattcg
     3121 gcgatatgac aagacagggt ctggtcttct taatttcatg accgatagtc ggatcaagat
     3181 ttatcagaaa tacatcgtat tgctccacta ccattgccag tctcgatctg cggtatctgt
     3241 gaaatcgggg atcaataact tatcatcccg gtgggtggcc atttctttaa atttctttgc
     3301 ccaaccggct cgcgccttac tttttaccgg ttttaataac agaccatctt tcgtaggggt
     3361 gatatcgact tcgtcttcga tatggcattg ctctaaaata cttttaggaa tcctcagtcc
     3421 tttcgagtta ccaactttca ccacccgtaa aatcatatca gaatagtaat tacattgtaa
     3481 tcacatcgtc aagtttttct atcctgaacg tgtaagcttc agacaataaa ttacatttac
     3541 ggcggtccct gcagtcacct gcgacacatc ttgtcttgta tcggtgatta caggcagatg
     3601 ccacttgcga aaccttctgg tagtagtttg acatgccaag gtgggggcgg ctgggagtcg
     3661 acgcggtggc cgcgcgggac gttacacgtc atacaggtaa catgatgcgc cagagtaaaa
     3721 tatacgtgta acacttgaca acgggtagat tacccgccgg gcagggaata gacgttgtgc
     3781 gacaaaccat caagagtatt tgactcatgg atagtgcaac caaact
//
LOCUS       DAOQJF010000317         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_14996_length_3789_cov_4.051955, whole genome shotgun sequence.
ACCESSION   DAOQJF010000317 DAOQJF010000000
VERSION     DAOQJF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14996_length_3789_cov_4.051955"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLF85_14100"
     CDS             <1..724
                     /locus_tag="PLF85_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20851.1"
                     /translation="VDISAAGVAPVGQVEATVLTGGSGCPVGAKVVIGENPFVTFSDG
                     GSAIVDTISGGQLTSVSVSEPAVNAAQLIQLIDRDATGITSGTRGYSITYNGTTPNIS
                     AREAMVRLLHHGVTIPAGSTKGYFSENYGLGSVVDVASDGLADNLKGGVALGGTGVWT
                     NAYAVNLPGLGPQHIAGSILNNLGGVEPTQTLINMVNTNTVDLTDTLLLLGCGDRSTY
                     TNAAVTSFSWQQLCTQLGPGIW"
     gene            767..2674
                     /locus_tag="PLF85_14105"
     CDS             767..2674
                     /locus_tag="PLF85_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE20852.1"
                     /translation="MNLLLAYLPFIALLVLSSSVFHIVYLNAAAPPVAWLIVLRYTLA
                     TAALAFFVPGLVYLFSPAGGRLRRAMLWLIGIITYYCLTVIVGDLVYYGQAGKHSTVE
                     LLLFYQNMIDVSLMALKDYTYIPVIYLVALLVFIRFWKRIVARERQQAPARRLSYLMR
                     ALLIAAFALGSLVFARGGLQGRPLRPADAFVHLTQAQGDFALNGVYTSFYAMFHRSSF
                     PVLADREAIARARALIAEKGEVFITDENPFYRISRRGTPLKKNVVFILLESWSAKRVG
                     VFGDKSGATPYFDSLAARGWLFTNAFATGRRSIASLPSALSSIPTLYGSLYITSPHEQ
                     NFQRGMGAIFRDAGYSTSFTYAAKAGSMGFNAFARLAGFESIITRESFPADAAHDGVW
                     GIYDHVMFERALADMRAAKKPFASVIYTLHPHPPFTLPPGYDYFSETGAGTRRNARAK
                     YFNALRYSDETLRNFFTAAEKEPWFKDTLFVLMADHAFEEKQGRDSYHIPLLFYSPGF
                     VPARRDDRVASELDVLPTIISLLKLPVAHASMGKSLEADGDRFAFIDIEHSGGFVTEK
                     NGQQLALLFGAEKYSGYYNMTSDPGWQQLVTGQPLTATTVQSLQDFIGVMGYAVARNR
                     IAPASKLETQP"
     gene            2757..3710
                     /gene="mdh"
                     /locus_tag="PLF85_14110"
     CDS             2757..3710
                     /gene="mdh"
                     /locus_tag="PLF85_14110"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNE20853.1"
                     /translation="MQHKRPKIALIGAGQIGGNLALMAVQKGLGDVVLFDVVEGVPQG
                     KALDLYESTPVDGYSNSLTGTNSYDDVKDADLVIITAGIPRKPGMSRDDLLQTNAKIM
                     TDVATNLKRVAPNAFVIVISNPLDAMVYLFQKVSGFAKNKVMGMAGVLDAARFRTFIS
                     METGVSVADISAFVLGGHGDTMVPLPRYSYVGGVPLPDYPGLTAQKIEEMVTRTRNGG
                     GEIVALLKTGSAFYAPAASAIAMAESILRDQRRVFPVAVYCDGEYGYKDMHIGLPVVL
                     GKNGVEKIIEIKLTAEEKAALDKSAEAVEGLKADLKRLNFL"
BASE COUNT          783 a         1040 c         1086 g          880 t
ORIGIN      
        1 tgtggatatt tccgctgctg gcgtagcacc ggttggtcag gtggaggcca cggtcttgac
       61 cggcggctca ggctgtccgg tcggagcgaa agtggtgatc ggtgaaaatc cttttgttac
      121 attctctgac gggggtagcg caatagttga tacgatcagc ggaggtcaac ttacctccgt
      181 cagcgtttct gaacctgctg tcaacgcagc gcagctgatt cagctgattg accgcgatgc
      241 aacaggcatt accagcggca cacggggcta ttcgattacc tacaacggca caactcccaa
      301 tatttctgcc cgcgaggcta tggtgcgtct gttacaccac ggcgtgacca ttccggcagg
      361 cagcaccaag ggatatttta gtgaaaacta tggcctgggc agcgttgtgg atgtcgcgtc
      421 tgatggcctg gctgataacc tgaagggtgg tgttgcgctc ggcggcacgg gtgtgtggac
      481 gaacgcgtat gcggtgaacc tgccgggcct tgggccgcag cacatcgcgg gttcgatcct
      541 gaataacctg ggtggcgttg agccgacgca aaccctgatc aacatggtga acaccaacac
      601 ggttgacctg accgatacac tccttttact cggctgcggt gaccgttcga cgtataccaa
      661 tgctgcggtg acttcgttta gctggcagca gctctgcacg caattgggcc ccggcatctg
      721 gtaatgaatc gacgctgcca cgggcagcgc cgatttgcct gttaacttga atcttcttct
      781 cgcttacctg cctttcattg cgctactggt tctcagtagc tcggtctttc acatcgtgta
      841 tctcaatgcg gcagcgccgc cggttgcctg gctcattgtc ctgcgctaca cgttggcgac
      901 tgcagcgctc gcattctttg tacccggcct tgtctacctg ttctcgcctg cgggtggcag
      961 gttgcgccgc gccatgctgt ggttgatcgg catcatcacc tactactgcc tcacggtgat
     1021 tgtgggtgac ctggtctact acggccaggc aggtaaacat tcgacggtgg agcttttgct
     1081 cttctaccag aacatgatcg atgtgagcct gatggcgctg aaggattaca cgtatattcc
     1141 tgtcatctac ctcgtagcgt tgctcgtatt catccgcttc tggaagcgca tcgtcgcgcg
     1201 tgaacggcag caggctccgg cacggcgcct gtcgtacctg atgcgtgcgc tgctgatcgc
     1261 ggctttcgcg ctcggttcgc tggtcttcgc gcgcggcggt ctgcaggggc gaccgctcag
     1321 gcctgcggat gctttcgtgc acctgacgca ggctcagggc gatttcgcgc tcaacggcgt
     1381 gtacacttca ttctacgcga tgttccaccg cagcagcttc ccggttttgg cagacagaga
     1441 ggcgatcgcg cgggcgcgcg cgctcatcgc cgaaaaaggc gaagtattca tcaccgatga
     1501 aaatcccttt taccgcatta gccggcgcgg gacaccgctg aagaaaaacg tggtatttat
     1561 cctgctcgaa tcgtggtcgg cgaaacgcgt cggagtgttc ggcgacaaga gcggggcgac
     1621 gccgtatttt gattcactgg ctgccagggg ctggttattc accaatgctt ttgccacggg
     1681 caggcgttcg attgcgtcgc taccctcggc gctcagcagt attccaacat tatatggttc
     1741 gctctacatc acttcgccgc atgaacaaaa tttccagcgc ggtatgggag cgatattccg
     1801 cgacgcgggt tattcgactt cgttcactta tgccgcaaag gccggttcta tgggttttaa
     1861 tgcctttgcg cggctcgcgg gtttcgagag tattatcacg cgggagagtt ttcccgctga
     1921 tgcggcgcat gacggcgtct ggggaatcta cgaccacgtg atgttcgaac gcgcgcttgc
     1981 cgatatgaga gcggcaaaaa aaccgtttgc ttcggtaatc tacactctgc acccgcaccc
     2041 accgtttacc ctgccgccgg gctacgacta tttctctgaa acgggcgccg gcacccggcg
     2101 taatgcgcgc gcgaagtatt ttaacgcgct gcgctattcc gacgaaacgc tgcggaactt
     2161 ctttaccgct gcggaaaaag aaccgtggtt caaagataca ctgtttgtcc tgatggcaga
     2221 ccacgcattc gaggaaaaac aggggcgcga ttcgtaccac attccgctgc tgttctattc
     2281 gcccgggttc gtgccggctc ggcgcgacga tcgtgtcgcg tcggaactcg acgttttgcc
     2341 gacgattatc tcgttgctta aattacccgt ggcgcatgcc tctatgggca agtcgctgga
     2401 ggctgacggt gaccggtttg cctttatcga catcgaacac tccggtggat tcgtcaccga
     2461 aaaaaacggg caacagctgg cgctgctctt cggggcagaa aaatacagcg gttactacaa
     2521 catgaccagc gacccaggct ggcagcagct ggtaaccggg cagccattga cggcaactac
     2581 ggtgcagtcc ctgcaggact tcatcggggt gatggggtac gctgtggcgc gtaaccgcat
     2641 tgcgccggct tcaaaacttg aaacgcagcc ttaattgacg cgctgagtcg acccgaaagt
     2701 ccatctgtta tcagataggc aaaagcgggg caggtgcccc aaatttaagg agaattatgc
     2761 aacataaaag accaaaaatc gcactgattg gtgcaggaca aattggtggt aacctcgctt
     2821 tgatggcagt acagaagggt ctcggcgatg tcgtgctttt cgatgttgtc gaaggtgtac
     2881 cccagggcaa agcactcgac ctctatgagt caactcccgt tgatggctac tctaactcgc
     2941 tcaccggtac caactcatac gacgacgtca aagacgcaga cctcgtgatc attaccgcag
     3001 gtattccccg taagccaggc atgagccgcg acgacctgct gcagaccaac gccaagatca
     3061 tgaccgatgt ggcgaccaac ctgaagcgcg ttgcacccaa cgcttttgtt atcgtgattt
     3121 ctaacccgct cgatgcgatg gtttacctgt tccagaaagt ttcaggtttc gcgaagaaca
     3181 aggtgatggg catggcgggt gtgctcgacg cggcacgttt ccggactttc atctcaatgg
     3241 aaaccggcgt gagcgtcgct gacatcagcg cctttgtgct cggcggtcat ggcgatacca
     3301 tggttccgct gccacgttac agctacgttg gtggcgttcc gctgcctgat tacccgggcc
     3361 tgacagcgca aaaaatcgaa gaaatggtaa cgcgtacgcg taatggcggc ggcgagatcg
     3421 ttgcactgct caagacaggc tctgcattct atgctccggc tgcttcagca attgcgatgg
     3481 cagagagcat tctgcgcgac cagcgccgtg ttttcccggt ggctgtttac tgcgacggtg
     3541 aatacggcta caaagacatg cacatcggtc tgccagttgt gcttggcaag aacggcgttg
     3601 agaagattat cgagatcaag ctgactgccg aagaaaaagc agcactcgat aagagtgccg
     3661 aggcagttga aggtctgaaa gctgacctga aacgtctcaa ttttctctga caagttccac
     3721 aaacaggggc cagaaaggta agggggagta atcccccttt tttgttgtgc aaagcagcgg
     3781 ttgatcgtc
//
LOCUS       DAOQJF010000318         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15007_length_3787_cov_4.385584, whole genome shotgun sequence.
ACCESSION   DAOQJF010000318 DAOQJF010000000
VERSION     DAOQJF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15007_length_3787_cov_4.385584"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLF85_14115"
     CDS             <1..979
                     /locus_tag="PLF85_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20854.1"
                     /translation="SINGGNTFNNSTSGLGGNDVRSLSVSGTTVYAATSNGISISINN
                     GTSFTNAIAGLGSSNTNGIFASGSTSTIHFSGYSAARASVIINEVCVTDCPTGTFNSK
                     DWVELKVKPSGAGSLRGLKLYYYDESEFRLVYEFSDVYAPANSLIAVGLAQAAESRPD
                     RNGATVAYPMAITLRDTAAFGATDGTFILTYCATGENDTVDTAQPGCALPYKGIQDAL
                     YYSNRDGTLTQPMVAGPLNHFYSNLQSFWPISERPVFGLNDRNTQLTGSCIAADSSST
                     ENSTYLCTNSGAGIARSSADTGAVRNAGKTGWRNYTRTAMTPNAANATW"
     gene            1047..1793
                     /locus_tag="PLF85_14120"
     CDS             1047..1793
                     /locus_tag="PLF85_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20855.1"
                     /translation="MLRATAIISFFFCASLWADGNISFRIGQRDYQLSGAHGMIAKRK
                     GKTQLVIAVKDTQHKTQFALTAELPADLTGPMELTPEVAPLTAVIVNARGIYSFVPHV
                     TLARDDFMRYTKKEEIATGELEDDPEDRPEERLEECRRGNFRDCLQAIKEKRRRKPKF
                     KVRYTKHAPTWVGKSREERITQGDGVAIEEKYRDTSLVVRLTPVLVNGKVTSLTGSFA
                     GVVVYNEGLNSATKVPLQGGAFSVTVKGAE"
     assembly_gap    1959..2058
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2059..2735
                     /locus_tag="PLF85_14125"
     CDS             <2059..2735
                     /locus_tag="PLF85_14125"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNE20856.1"
                     /translation="RMWTARDAVDDIERLKKVFIGQSRFDIRPTQVIDVFIIDPSTQK
                     HKVVDMQWGWKPDWTKGKPIMNTRKEGVAGNRLWGTALRERRCVVPATHFYEWQRRDD
                     SPRNVPWMIKRKAHDHMYFAGLWNMRQSPGTDAETFEVSIFTEPGNSLMTEIHNHGGN
                     FGRQPVFIDEERIPDWLDPALKDPEKALMLLRPIEDGEYEAEMLEEIGNDKTHSPPKP
                     RQANLF"
     gene            complement(2739..>3787)
                     /locus_tag="PLF85_14130"
     CDS             complement(2739..>3787)
                     /locus_tag="PLF85_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20857.1"
                     /translation="PEPEAKAEASDEAVDLKALGDEMAGLWGEKVCVAEPGSKHEEQV
                     ISIACNAEYTSAERDKVLDFLFKKGFLFRGESYDIKGTTGHYWYRFEHREGNRLRSVF
                     VRMHITSQGALWGSVKPPARIAVYVDNLATADELTAWQTLGIPLSFGLKPSESAKELA
                     QQIDEYKQEAWLALDLRQGAFTEPDQSASVRDIIEQDLIPNHLKNSLEQTGDVWGVVV
                     RDLNNITTTVATARAVFSAMKAEGKSYVLLPAKYNKALSTTANVMDMNARRITLDMGG
                     MCAKSPSKIWSYVRSKAGHGQIIVRFPANAKRCAHTLSRTLRRDGKTEFRPLSTFFGY
                     AEGATTEKKAGAGN"
BASE COUNT          841 a         1048 c         1005 g          793 t
ORIGIN      
        1 atcgattaat ggtgggaata cgttcaataa ctcaacgtcc gggttgggcg gtaacgatgt
       61 ccgaagtctg tctgtaagtg gaacaacagt ttatgctgcg acctcgaatg gcatctcaat
      121 ctctataaat aatgggacat cattcacgaa tgcgatcgca ggacttggta gctccaatac
      181 gaatggaatc tttgcaagcg gctctactag caccatccac ttcagcggct attcggctgc
      241 gcgcgcttcg gtgatcatca acgaggtctg cgtcaccgac tgcccgaccg gcacattcaa
      301 cagcaaagac tgggtagaac tcaaagtcaa accctccggt gcaggttcac tgcgcggcct
      361 gaaactttat tattacgacg aatcagaatt cagactcgtg tatgaatttt ccgacgtgta
      421 cgctcccgcg aactcgctga tagctgtcgg gcttgcgcag gccgctgaaa gccgccccga
      481 ccgcaatggc gcgacggttg cctacccaat ggccatcacg ctgcgcgata ccgctgcttt
      541 tggcgccacc gacggcacct tcattctgac ctactgtgcg actggcgaga atgacaccgt
      601 cgacaccgcg cagcccggtt gcgcgctgcc ctacaagggc atccaggatg cactctacta
      661 cagcaaccgt gatggcaccc tgacgcaacc catggtggct ggcccgctca accatttcta
      721 cagtaacctg cagtctttct ggcccatcag cgaacggccc gtctttgggc tgaacgaccg
      781 caacacgcag ctgaccggca gctgtattgc cgccgatagc agctccacag aaaactcgac
      841 gtacctctgc acgaattcgg gtgcgggtat tgcccggtcg tctgcggaca ccggggctgt
      901 gcgcaatgcc ggtaagaccg ggtggcgaaa ctatacccgc accgcaatga ctcccaatgc
      961 cgctaacgcg acatggtaga aaaacgtttg ttcaccaata aaatggcacg ctccgccctg
     1021 taccgtgcat agctatatgg taagttatgc tccgcgcgac tgcgatcatc tccttcttct
     1081 tttgtgcgtc gctgtgggca gacggtaata tttcattccg cattggccag cgtgactacc
     1141 agctttcagg cgcacatggc atgatcgcca agcgcaaggg caagacccaa ctggtgatcg
     1201 cggtaaaaga cacgcagcac aagacacagt ttgccctcac tgcagaactg ccagccgacc
     1261 tgacaggccc catggagctg acacccgaag tggcgccgct cactgcagtc atcgtcaatg
     1321 ctcgcggcat ctacagcttc gtgccacacg tgacgcttgc gcgcgacgac ttcatgcgct
     1381 ataccaaaaa agaagaaatc gcgacgggtg agctcgaaga tgaccctgaa gaccggcccg
     1441 aagagcgcct ggaagaatgc cgccggggca acttccgcga ctgcctgcag gccatcaaag
     1501 aaaagcgccg gcgcaaacca aagttcaagg tccgttatac caaacacgca ccgacatggg
     1561 tcggcaaaag ccgcgaagag cgtatcacgc agggcgatgg tgtagcgatc gaagaaaaat
     1621 accgcgatac ttcgctggtg gtccgcctta cccccgtgct ggtgaacggt aaagtcacca
     1681 gcctgacggg cagctttgcg ggtgttgtcg tctataatga gggtcttaac tcagcgacca
     1741 aggttcccct gcaaggtgga gctttttcgg ttacagttaa aggcgccgaa taggactcca
     1801 ctgccccgcc acacgcggtg cccgaaccac ctgagtagaa ttcgtatgtg cggtcgaatg
     1861 gttctcatca agtacgttaa ctttgacgga accattggct attaccgcat gtggactgca
     1921 cgggatgcag tcgacgatat cgaacgcctg aagaaagtnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnac cgcatgtgga ctgcacggga tgcagtcgac gatatcgaac
     2101 gcctgaagaa agtttttatt ggccagtcgc gctttgatat acggccgacg caggtcatcg
     2161 atgtcttcat catcgaccca tcgacacaga aacacaaggt cgtcgacatg cagtggggct
     2221 ggaaacctga ttggacgaaa ggcaaaccga tcatgaacac gcgcaaagaa ggcgtcgcgg
     2281 gcaaccggct ctggggtacg gcattgcgcg agcgccgttg tgttgtaccc gccacacatt
     2341 tttatgaatg gcagcggcgt gacgattcac cccgcaatgt accgtggatg attaaacgta
     2401 aagctcatga ccacatgtac ttcgcagggc tgtggaatat gcgccaatca cctggtaccg
     2461 atgctgaaac tttcgaggtt tccatattta ccgaacccgg caacagcctg atgacagaaa
     2521 tacataacca tggtggcaac ttcggtcgcc agcccgtgtt catcgacgaa gaaagaattc
     2581 ctgactggct tgatcccgca ctaaaagacc cggaaaaagc cctgatgctt ctgcgcccga
     2641 tagaggatgg tgagtacgaa gcggaaatgc tagaagagat cggcaacgac aagactcaca
     2701 gcccgccgaa accccggcag gctaatttgt tctaaacctt aatttccagc gccggctttt
     2761 ttttccgtcg tcgcaccctc ggcgtagccg aagaatgtgc tgagcggacg gaattcggtc
     2821 ttgccatcgc gccgcagtgt gcggctgagc gtgtgagcgc agcgctttgc gttcgccggg
     2881 aaacggacga ttatctggcc gtgaccagcc ttgctacgca cataactcca gattttcgac
     2941 ggtgacttag cgcacatgcc gcccatatcg agggtaatac ggcgtgcgtt catgtccatg
     3001 acgttggcgg tagtgctcag agccttgttg tactttgctg gcagcaggac gtaggactta
     3061 ccttcagcct tcattgctga aaaaactgcg cgcgcggttg ccacagtggt ggtgatattg
     3121 ttgaggtcgc gtactacaac tccccagaca tcgcccgtct gttcgagcga gtttttcagg
     3181 tgattgggta tgagatcctg ctcgatgata tcgcgcaccg atgcgctctg gtctggctcg
     3241 gtaaaagcgc cctggcgcag gtcgagtgcc agccatgctt cttgcttgta ctcgtcgatc
     3301 tgctgcgcaa gttctttcgc gctttccgaa ggtttcaggc cgaagctcag gggaattccc
     3361 agtgtctgcc aggccgtcag ctcgtccgcg gtcgcgaggt tgtctacgta gacggcaata
     3421 cgtgcgggag gtttaacaga accccagagt gcaccctggc tggtgatgtg catgcgcaca
     3481 aaaaccgagc gcaggcgatt gccctcgcgg tgttcaaaac ggtaccagta gtgccccgta
     3541 gtacccttga tgtcgtatga ctcgccgcgg aacaggaagc ctttcttaaa gaggaaatcg
     3601 agcaccttgt cgcgttctgc cgaggtatat tcggcattgc aggcgattga aatgacctgc
     3661 tcttcgtgct tgctgccggg ttcggcgacg cagacttttt caccccacag gccggccatc
     3721 tcgtcgccca gtgctttcag gtcgaccgct tcatcagaag cttctgcttt tgcttctggt
     3781 tccgggt
//
LOCUS       DAOQJF010000319         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15092_length_3774_cov_5.166711, whole genome shotgun sequence.
ACCESSION   DAOQJF010000319 DAOQJF010000000
VERSION     DAOQJF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15092_length_3774_cov_5.166711"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLF85_14135"
     CDS             <1..664
                     /locus_tag="PLF85_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20858.1"
                     /translation="GERMGKPGILLTLVLCPLFFCHCAENRLTTYMLVQQQGTGSTQK
                     KGQVQLSAAPLLLTVVVPDPEELEELAEDLKLATVSKYHRLPPLTYLRFNFENRSALG
                     WQLNLGAVRFTAGKRSFGVVKTADYTARFTSVAYEHFRYDAMFASYITRRGDGRPAEN
                     FWFAKRAPHEIIDLGANEAGFQVLPFEFIPPGIDELTLHYTLEGQVEKTLTVKLVTER
                     GS"
     gene            666..1280
                     /locus_tag="PLF85_14140"
     CDS             666..1280
                     /locus_tag="PLF85_14140"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNE20859.1"
                     /translation="MQNQLAIFAALVALAVLTKLWARRAYAFAKPLPVVFILFLVFLL
                     PAKPPAIWLVGLFFGLAGDLFLLSSRGFLPGLISFLLGHVFYIWAFTQQQPAAPDTSL
                     AVVVGLSAAAIYAYFARLLLASRKKKYLVPVLLYVGVTGVLVVKAIQSGIVYPAVLGV
                     LLFALSDFLLAFNKFSRQSWYVEAGVSLTYFPAQWLLAAHFAGI"
     gene            1332..1649
                     /locus_tag="PLF85_14145"
     CDS             1332..1649
                     /locus_tag="PLF85_14145"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HNE20860.1"
                     /translation="MEHVKITRIQVFPQNDGRNTIGYAHVTLSDAFVVKNLRLVKGKK
                     GVFLGMPSFRLRNGEYADVFFPVSREARAMLTEAVVAAFRQEHPELMEGLEIYDADAA
                     GVI"
     gene            complement(1646..2005)
                     /locus_tag="PLF85_14150"
     CDS             complement(1646..2005)
                     /locus_tag="PLF85_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20861.1"
                     /translation="MPAGAGVAGAGSAFAAMHALRASPNAALADFMHSAFSLPFFALH
                     SLVAAFFEATAFFTHAFCSADGRFAESAVTANTAATITSETFFMINVLLWTLRPQDRR
                     MRPSPADSKFQAPEKRI"
     gene            2218..>3774
                     /locus_tag="PLF85_14155"
     CDS             2218..>3774
                     /locus_tag="PLF85_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNE20862.1"
                     /translation="MSGLAVKVATVSAPGSFVQTVKSCAHCGASGASILRGAENFCCE
                     GCAQVYSILKDQDMLGFYEIPDNAVRSLKEKPTADYSYCDSEWFRRMFVRPWGKMGWS
                     LRMRLPDIHCAACVWLLEKLPEMLGGVTSARINYLRKQIDISASGDLPVSQLVQFVAN
                     LGYPPDFAKESAASRRLSAYDKSLLRKMAVAAFGFGNAMLFSLPEYFTAHLESSFSRT
                     FMALNAALSVVVLIYSAGDFFRSAWRALKQRQIVIDVPISVGIAAMFTRSAIDVATGA
                     SAGYFDTFTGLIFFLLIGRFVQSRSYAWLNFERDNLLFLPLAVRVRTESGEAVTPVQQ
                     VKRGDTLRLLAGEIAPTRCRVISDEGAADYAFITGESLPVTLKRGDVFEPGGKVVGQS
                     VLLEVLEEVDQARLNRIWESAEPESEAETYTYGSSFAERVLPYFTGTVIAIATGALLY
                     WLPSDGARAWNTFSAVLVITCPCALAMAKPFSFFTTQSVLARAGLFLKSAAVVERFFA
                     LKNIVFDKTGT"
BASE COUNT          766 a         1025 c         1086 g          897 t
ORIGIN      
        1 gggggagcgc atgggtaaac ccggcatctt actgaccttg gtgctctgcc ccctcttctt
       61 ctgccattgt gccgaaaatc gtctgaccac ctacatgctg gtgcagcagc agggtacggg
      121 cagtacgcaa aaaaaagggc aggtgcagct ctcggcggca ccgctgctgc tcaccgtggt
      181 cgttcccgac ccggaagagc tggaagaact tgccgaagac ctcaagctgg cgaccgtttc
      241 gaaataccac aggctgccgc ccctgacata cctgcgcttc aatttcgaga accgcagcgc
      301 cctgggctgg cagctcaacc tcggtgcggt ccgtttcaca gctggcaagc ggagctttgg
      361 ggttgtgaag actgcagact atacggcacg attcacctcg gttgcctatg agcacttccg
      421 ttacgatgcg atgtttgcct cgtatattac caggcgcggc gatggtcgtc ctgcggaaaa
      481 tttctggttt gccaaacgcg ctccgcacga gataattgac cttggggcaa atgaagccgg
      541 cttccaggtg ctgccctttg agtttattcc cccgggtatt gacgaactta cgctgcacta
      601 cacactcgag gggcaggtcg aaaaaacact gacggtgaag cttgtcaccg agcgagggag
      661 ttaaaatgca gaaccagttg gcaatatttg cggcgcttgt agcgctggcg gtgttgacga
      721 aactttgggc gcgcagggca tacgcctttg ccaagccact gccggtagtt ttcattcttt
      781 tcctggtttt tctcctgccg gccaaaccgc cggctatctg gctcgtgggg ctgttctttg
      841 gcctggcggg tgacctgttc ttgctttcct cgcgcgggtt tttgcccggg ctgattagct
      901 ttctcctggg acatgtattc tatatctggg ccttcacgca gcaacaacct gcggcgcccg
      961 ataccagcct cgcggtggtc gtcggtcttt cagccgcagc gatctatgct tattttgccc
     1021 gcctgctgct ggcctcgagg aaaaagaagt acctggttcc tgtattactc tacgtcgggg
     1081 tgaccggtgt gctggtcgta aaggccatac agagtgggat agtgtacccg gcggtactgg
     1141 gtgtcctatt gtttgccctg tcagattttt tgttggcatt taacaagttc tcacgtcaga
     1201 gctggtatgt tgaggcgggt gtctcgctca cttattttcc ggcgcagtgg ctgcttgcag
     1261 cgcattttgc cggcatataa ccgaaagctc cgccgcacgc aaacctacag ctaaccttgg
     1321 gagacaatta catggaacac gtcaaaatta cccgcattca ggtatttcct cagaacgacg
     1381 gcagaaacac gataggctac gcccacgtta ccctctctga tgccttcgtg gtaaaaaatc
     1441 tgcgccttgt gaagggcaag aagggagttt ttctgggtat gccgtctttt cgcctgcgca
     1501 atggtgaata cgctgacgtg ttctttcctg tgtcgcgtga agcgcgtgcg atgctgacgg
     1561 aagccgtggt ggctgccttc aggcaggagc accccgaact catggaaggc ctcgaaatct
     1621 acgacgccga cgccgcaggg gtcatctaaa tccgtttttc tggggcctga aatttgctgt
     1681 ccgccggtga cgggcgcatg cgtctatcct gtgggcgaag agtccacagg agtacattga
     1741 tcatgaaaaa agtttcactc gttatggttg cagcagtatt tgctgtgact gcgctttctg
     1801 cgaaacggcc atctgccgaa cagaaggcat gtgtaaaaaa tgcagtagct tcaaagaatg
     1861 ctgctactaa agaatgcaaa gcaaagaaag gtaaagaaaa agcagagtgc atgaagtctg
     1921 ccaatgcggc atttggcgac gcgcgcaaag cgtgcatggc ggcaaaagct gagccagcac
     1981 ctgcaactcc agcgcctgca ggcaaataac cgttttccag gccgccgagt ctgccggcag
     2041 agtggctggc agcggcggcc ttttttctgc ctttgcccgc ccgggcaaaa aagcggcgac
     2101 agcccgatag ctctataagc acttgccagc agtcttcagc gcataccagt caataacaga
     2161 ctcgccggta taaataaaaa aagacctatt aatcgtttac gcagtcgggg ggtgtttatg
     2221 agtggccttg cagtgaaagt tgccaccgtg agcgcgcctg gttcgtttgt gcagaccgtc
     2281 aaatcctgcg cgcattgcgg tgcgtccggc gcgtcgatac tgcggggtgc agaaaacttc
     2341 tgctgcgaag gctgtgcaca ggtctattct atccttaaag accaggatat gctcgggttt
     2401 tatgaaatac ccgacaatgc agtccgctca ctcaaagaaa aaccgacagc agactacagc
     2461 tattgcgaca gcgaatggtt tcgccgcatg tttgttcgtc catggggcaa gatgggctgg
     2521 tccctgcgca tgcgcctgcc cgatatccac tgtgccgcct gtgtctggct gctcgaaaaa
     2581 ctgcctgaaa tgctgggggg agttacctcg gcgcgtataa actacctgcg caagcagata
     2641 gatatctcgg cttctggtga tctgccggtc tcgcaactgg tgcagttcgt ggcgaatctt
     2701 ggctacccgc ctgactttgc caaagagagt gcggcgtcac gccggctgtc ggcatacgac
     2761 aagtcgctgc tgcgcaagat ggcggtcgct gccttcggtt tcggcaatgc gatgctcttc
     2821 agtctgcctg aatatttcac ggcgcacctc gaatcatctt tttcgcgcac cttcatggcg
     2881 ctgaacgcgg cgctgtcggt tgttgttctc atctattcag ccggcgactt cttccgcagc
     2941 gcctggcgtg cgctgaagca gcggcagatt gtcatcgacg tacccatatc ggtgggtatt
     3001 gccgcgatgt tcacgcgcag cgcgattgat gtcgcgacag gcgccagcgc gggttacttt
     3061 gataccttca cgggattaat cttttttctg ctcattggcc gctttgtaca aagccgctcg
     3121 tatgcgtggc tcaactttga acgcgataac ctgctcttcc tgccgctggc ggtgagggtg
     3181 agaaccgaga gcggcgaagc cgttaccccg gtgcagcagg taaagcgggg tgatacgctc
     3241 aggctgctgg cgggtgaaat tgcgcccacg cgctgccgcg tgatctctga cgaaggggct
     3301 gcagactatg cgtttatcac cggcgagtcg ctgcccgtga cgctgaagcg cggcgatgtc
     3361 tttgagccag gtggcaaggt tgtggggcag tcggtactgc tcgaagtgct tgaagaagtc
     3421 gaccaggccc gtctcaaccg catctgggaa tccgcagagc ctgagtccga agccgaaaca
     3481 tacacctatg gcagttcatt tgccgaaagg gtgctgcctt attttaccgg cacagtcatc
     3541 gcgatcgcta cgggagcgct gctgtactgg ttgccaagtg acggtgcccg tgcctggaac
     3601 acattttccg cagtactggt gattacctgc ccgtgcgctc ttgccatggc gaagcctttc
     3661 agctttttta ccacgcagag tgtgctcgcg cgggccggcc tgttcctgaa atcagctgca
     3721 gtggtggagc ggttctttgc gctgaagaat atcgttttcg acaagaccgg cacg
//
LOCUS       DAOQJF010000320         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15164_length_3763_cov_5.110572, whole genome shotgun sequence.
ACCESSION   DAOQJF010000320 DAOQJF010000000
VERSION     DAOQJF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15164_length_3763_cov_5.110572"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2410
                     /locus_tag="PLF85_14160"
     CDS             <1..2410
                     /locus_tag="PLF85_14160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNE20863.1"
                     /translation="HTVRRIISVFARAALSLAQLVLGLDAHQRWARLAGSALGLFTVF
                     VLSLVLISQAWRAFHLEEYKPSVPSELYDRKGRLITTFFQDQRILVSEKDIPSHLKQA
                     FIAMEDNHFYDHFGISPQGIFRAFLINLQAGQVKQGGSTITQQLAKVVLTDQKRTFTR
                     KIKEAALALIIDAIYAKDKILHLYCNQIYFGHGNYGVEAAAQFYFEKPVSEITPAQAA
                     ILATLPSSPNRYSPLRNPHLSRARLQHALMKMVDLGFLQIADAQKAEAEAVAYYGSLN
                     IAPTETAFGRRIDNAPYFSEYVRPILEKEFGKQALYNEGLKIHTGLDLDHQRAAQEAL
                     WRGVRAQNAISRNYFFSRQLELSREYAPLLGLAQLAMNLPELNPAKRFDQYELHMRFF
                     EEYLDPLELVNLGFGGNNELDQMLISLRADNPFANRYLAVQGALAEIDNATGEVTAVV
                     GGLPFSTQNQVNRALQMERQPGSAFKPILYAAALETKKITAATQFPDSPIVSIDEQGD
                     LWMPENYHAGFRGFITLREALRYSVNTVSIVVAREVGLGNLIPVIAKELYVTPNQIPY
                     NLSIALGTHEVSPLGMARGFSHFARGGLSIEPVLLKQVYDRNKKLLKDFTPQQKNIQI
                     ISGGTATIVRSLLETAVNKGTGNTVRKTGYTGYAAGKTGTTNGYRDAWFVGFNKRYTA
                     AVWVGYDRPSLSLGPGQAGAGVAAPIWANFQNRVSAAIAKDDPHLKSVELTEVLYCSS
                     NGLPVRVGCDEPYRELFLEGTGPETGNNIDLGETIETEVQLPVSVEKKPVVKTKDFFE
                     GEDE"
     gene            2415..>3763
                     /gene="dnaX"
                     /locus_tag="PLF85_14165"
     CDS             2415..>3763
                     /gene="dnaX"
                     /locus_tag="PLF85_14165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNE20864.1"
                     /translation="MSLATRYRPQNFEDLVGQLAAAQSLKNALEFKKIGHAYLFHGAR
                     GVGKTTMARILAKALNCVNGPTSTICNQCQHCTEISEGRSPDVIEIDAASNRGIDNIR
                     ELRAQAVFAPMNARYKVYILDEVHMLSTESFNALLKTLEEPPHHVVFIMATTELHKIP
                     ETIASRCQVYTFKKFNAREIADRLAHILGKEQIAFEREALLPIAERGEGSLRDAISLL
                     DQTLAFSGSNTLTLAAVRESLDLLPSEVYATFLHGLRTGDLHALLNTVHELSQGGANL
                     RVFLRDLLAYLRAIVLVSEQVEVHALSKEEMEQARKAAQVWDRAQLIRVFQQLFRLNQ
                     DFSQMLSAKSSELRMSLEIALVDLVQKLKEPSVSALVQKIERLKTAIETGKPFDDAVP
                     QAATVLPLPPAIPEKKVEAVAPVTAPTLAEGGLDTATVLQKAFLAEEVAVNPETEKL"
BASE COUNT          840 a         1096 c         1043 g          784 t
ORIGIN      
        1 ccataccgtg cggcggatca tctcagtatt tgcccgggct gcgttgtcgc tggcccagct
       61 tgttcttggc ctcgatgcgc accagcgttg ggccaggctt gccggctcgg cgcttggctt
      121 gttcaccgta ttcgttctgt cactcgtgct gatcagccag gcctggcggg ccttccatct
      181 tgaagagtac aagccttcgg tgccgagcga gctctacgac cgcaagggca ggctgatcac
      241 tacatttttc caggaccaga ggatactggt cagcgagaaa gacattcctt cgcaccttaa
      301 gcaggctttc atcgcgatgg aagataacca cttctacgac cactttggta tttcaccaca
      361 aggtatcttc cgggccttcc tgatcaacct gcaggcaggc caggtgaagc aaggggggtc
      421 gacgatcacc cagcagctgg ccaaggtggt cctcaccgac cagaaacgta ccttcacccg
      481 gaaaataaag gaagcagccc tcgcactgat tattgatgca atctatgcca aggataagat
      541 actccatctc tactgcaacc agatctattt cggtcacggt aactatggcg tcgaggctgc
      601 agcgcagttc tactttgaaa aacccgtcag tgaaataact cccgcgcagg cagcgatact
      661 cgccaccctg cccagctcgc ccaaccggta ttcgccgctc aggaacccgc acctttctcg
      721 cgcgcgcctg cagcacgcgc tgatgaaaat ggtcgatctc gggttcctgc agattgccga
      781 cgcgcaaaag gccgaagccg aagccgtggc atactacgga tcgctcaata ttgcgccgac
      841 cgaaaccgcg tttggccgcc gcatcgacaa cgcgccttat ttctccgagt acgttcgccc
      901 gattctcgaa aaagaattcg gcaagcaggc gctctacaac gagggattaa aaatccatac
      961 ggggcttgac cttgatcacc agcgcgctgc acaggaagcc ctgtggcgcg gggtcagggc
     1021 gcagaacgcg atttcgcgca actacttctt ctcacgccag ctcgagctat cgcgcgagta
     1081 cgcgccgctg ctgggccttg cacagctggc aatgaacctg cccgagctca acccggcaaa
     1141 acgttttgac cagtacgagc tgcacatgcg gttctttgaa gagtaccttg acccactcga
     1201 actggtcaac ctcggctttg gcggcaataa cgagctcgac cagatgctga tctctctgcg
     1261 cgccgataac ccgtttgcca accgttacct tgcggtgcag ggggcgctgg ctgaaatcga
     1321 caatgctacg ggtgaagtca ccgctgtagt aggtggcctt ccgtttagca cccagaacca
     1381 ggtgaaccgt gcgctgcaga tggaacgcca gccgggctct gcttttaagc cgattctcta
     1441 cgccgccgcc ctggaaacca agaagatcac cgccgccacg cagtttcccg attcgccgat
     1501 cgtttcgatt gacgagcagg gcgatctgtg gatgcccgag aactaccacg ccggctttcg
     1561 tggttttatt acgctgcgcg aggctttgag gtattcggtg aatacggtca gtattgtcgt
     1621 tgcgcgcgaa gtcggtttgg gtaacctgat accggtcatt gccaaagaac tctacgtaac
     1681 tccgaaccag attccttaca acctgtcgat tgccctgggt acgcacgagg tatctccgct
     1741 cggtatggcg cgcgggtttt cgcactttgc gcgcggcgga ttgagcattg aacctgtgct
     1801 gctcaagcag gtatatgatc gcaataaaaa gctgctgaag gattttactc cgcagcagaa
     1861 gaatatccag atcatcagcg gaggcacagc aactattgtc cgctcattac tcgagaccgc
     1921 cgtcaacaag ggcacgggaa ataccgtgcg caagaccggt tacacgggct acgcagcggg
     1981 taaaacagga actaccaacg gttaccgtga tgcctggttt gtcggtttca ataaacgtta
     2041 tactgcggca gtctgggttg gttacgatcg cccgagcctt tccctggggc cggggcaggc
     2101 cggtgccggt gttgccgcgc cgatctgggc gaatttccag aatcgtgtgt ccgccgctat
     2161 cgccaaggac gatccgcatc tcaagagtgt tgaactgacc gaagtattgt actgcagcag
     2221 caatggcctg ccggtgcgtg ttggttgtga cgaaccatat agagaactct ttctcgaggg
     2281 tacaggtccc gaaacaggca acaatatcga cctgggcgaa acgatcgaga ctgaagtgca
     2341 gttgcctgtc agcgtcgaga aaaagccggt ggtcaagaca aaagacttct ttgaagggga
     2401 agacgaataa ccgcatgagt ctcgctaccc gctaccggcc gcagaatttt gaagaccttg
     2461 tcgggcagct tgccgcggcg cagagcctga agaatgctct cgaattcaag aagatcgggc
     2521 atgcatacct ctttcacggg gcgcgcggtg ttggtaagac gacgatggcg cgtattctcg
     2581 ccaaggcgct caactgcgtc aacgggccta cttccacaat ctgcaaccag tgccagcatt
     2641 gtacagagat atccgaaggc cgctcgcccg acgtgattga aatcgacgcg gcttcaaacc
     2701 gcggcatcga taatatccgc gaattgcgcg cacaggcggt ttttgcgccg atgaacgccc
     2761 gctacaaggt ttacatcctg gacgaggtgc acatgctgag caccgaatct ttcaacgcgc
     2821 tgctcaaaac tcttgaagaa cccccgcacc acgtggtctt tatcatggcg acgacggagc
     2881 tccacaaaat tcccgaaacg atcgcttcgc gctgccaggt atataccttc aaaaaattta
     2941 acgcgcgcga aatcgccgat cgcctggcgc acattctcgg caaagaacag atcgcgtttg
     3001 agcgcgaggc gctgctgccc atcgccgagc ggggcgaagg gtcgttgcgc gatgccatat
     3061 ccttactcga ccagacgctg gccttttcgg gcagtaacac gctcactctg gcagcggtgc
     3121 gtgaatctct cgacctgctg ccgtcagaag tttacgcgac tttccttcac ggcctgcgca
     3181 caggcgacct gcatgcactg ctgaacacgg tacacgagct ttcgcagggg ggggccaacc
     3241 tgcgggtttt tctccgcgac ctgctggctt acctgcgcgc catcgtcctg gtcagcgaac
     3301 aggtcgaagt gcatgccctg tcaaaagaag aaatggagca ggcccgcaag gcagcgcagg
     3361 tctgggaccg cgcacagctg attcgtgtgt tccagcagct gtttcgtctc aaccaggatt
     3421 tttcacagat gctttctgcc aaaagcagtg agctgcgcat gtcgctcgag attgcgctcg
     3481 ttgacctggt gcagaaactg aaggaacctt cggtcagcgc gctggtacaa aaaattgaac
     3541 gcctgaagac ggcgatcgaa accggcaaac catttgatga tgccgtaccg caggctgcta
     3601 cggttttacc cctgccgccg gcaatacccg agaagaaggt cgaagccgtc gccccggtga
     3661 ctgcaccaac gctggcggag ggtgggctgg ataccgcaac ggtgctccag aaggcctttc
     3721 tcgccgaaga ggtggcagtg aatcctgaga ccgaaaagct ctt
//
LOCUS       DAOQJF010000321         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15165_length_3763_cov_4.917476, whole genome shotgun sequence.
ACCESSION   DAOQJF010000321 DAOQJF010000000
VERSION     DAOQJF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15165_length_3763_cov_4.917476"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..978
                     /locus_tag="PLF85_14170"
     CDS             76..978
                     /locus_tag="PLF85_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE20865.1"
                     /translation="MKNLFLVTSFVATTIIVAQAPNSPSTASPQERLEAAVYADNPAE
                     IKAAIRAGANANGGNYSGHYLGIAATGGRLNAVKALIEAGADTNYASSGGWTAAMAAA
                     DNGHLEVLRYLALKKADLNARTRMGRTLLMRASYHGQTAVVKYLLTKGNKVADVDMNG
                     VTALMLAGQQGYDKTVKVLLDAGADRAAVNSAQKTALVLAREAQAANGNYRADEYKRT
                     IALLSSEKKSLGKLIGKIFRADGKKLEITGEGISAARRGTKLKIKTDSGEISAIVSET
                     LHSKIKANAAKAGASKGDAVYLEK"
     gene            978..1409
                     /locus_tag="PLF85_14175"
     CDS             978..1409
                     /locus_tag="PLF85_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20866.1"
                     /translation="MTGFLKRQADMVRQWVRSQPRLLAWGERTLPELEKFVIAGPTLV
                     GFVLGLVRYQPGTVHFETGFQSLVIFLAYLAALTVLQMADSLLFSSWPIVLGVARSLL
                     AVVYLAVTIRQFVQWRTGTPQIFPAVQHIRERLRPVTGDAA"
     gene            1399..2220
                     /gene="ttcA"
                     /locus_tag="PLF85_14180"
     CDS             1399..2220
                     /gene="ttcA"
                     /locus_tag="PLF85_14180"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNE20867.1"
                     /translation="MLPDTLERDSARHRLRKIPAAFRKEYKDILHEVGSLIAEYSLIE
                     QGDRVMVAVSGGKDSHALLHILLDLQAKAPIDFSVVAYTLDQKQPGFDASSLIAYYEE
                     IGVEYVLGERDTYSIVTEKIPAGQTFCSLCSRLRRGILYDEAQRLKANKIALGHHAND
                     ALETLLMNLFYSGKLAAMPPRLISDDGRNVVIRPLLRVTEDALAALAEQLNFPILPCN
                     LCNNQEGLQRARIKQLIAEEQKRNPNLLHSARGAMANVKPSHLWDLSLSTARDAE"
     gene            2226..>3763
                     /gene="nadC"
                     /locus_tag="PLF85_14185"
     CDS             2226..>3763
                     /gene="nadC"
                     /locus_tag="PLF85_14185"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNE20868.1"
                     /translation="MAAAKEKPGKIVPLFAGLRRKRAKTQAASQQSGTQLFRQVVTYV
                     RDRAKERHIGWSLIPALVTLRIVAAENLRAIRAAFEEGISAERPVHPDLLGEFLELRR
                     YFTAAIVYKAAENWKRQHADKKRTRQLDELLEELQFLSENQSWDWVSFHETEFEFFLQ
                     IAYLSRFTTAKFVLNRIRRDYLDSVLAYNKHFWNMETAVSVYRTVVNLIRESKPAEAE
                     GELLQFFTQNDLAILQYYTSQVAQRGFELATAPQRDRERPVYASNVPVLGDVEIGRAV
                     RYALAEDKASADITTQAIFTESARSTGRIFVKTDCVVCGIRVAEQTFRYFDTGMVIRT
                     AAADGDLLKAGSTVLEFEGDIRAVLRAERVALNFLAFMSSIATRTQQISRAAAVYGVK
                     VLDTRKTVPMLRAISKYAVVRGGGYNHRHDLSEMGLIKDNHIAQAGSVTAAILAFKRH
                     APFTPLEVEVDTLEQLQEALPLKPEMILLDNMDNATMKQAMKLFREANTAMGTKITAE
                     ASGG"
BASE COUNT          846 a         1068 c         1082 g          767 t
ORIGIN      
        1 aaaagctctt ctgaaatcgg gttgaacgcc tgtccgcttt cgttgaatag cacttattct
       61 ttccctggag gccctatgaa aaatctcttc cttgtaactt cctttgtcgc gacaactatc
      121 atcgttgcgc aggcacccaa cagtccatcc acggcgagtc cgcaggagcg gctcgaagca
      181 gccgtctatg cagataatcc ggcagaaatt aaggctgcga tcagggccgg tgcaaacgcc
      241 aacggcggaa attactcggg tcactacctc ggcattgcgg caaccggcgg acgcctgaat
      301 gccgtcaagg cgctcattga agcgggtgct gatacgaact acgccagttc cggtggctgg
      361 acggcagcga tggcagcggc agacaacggg cacctcgaag tgctgcgcta ccttgcactc
      421 aagaaggctg atctcaatgc ccgcacccgc atgggccgca cgctgctgat gcgtgcatct
      481 taccacggtc agacagcagt cgttaagtac ctgctgacga aaggcaacaa ggttgctgac
      541 gtcgacatga acggagttac cgcgctcatg cttgctggcc agcagggtta tgacaaaacc
      601 gtaaaagtcc tgctcgatgc cggcgccgac cgcgcagcag tgaattcagc gcagaagact
      661 gccttggtgc tggcccgcga agcacaggcg gcaaacggca actaccgggc agatgaatac
      721 aagcgcacca tcgcgctgct cagctctgaa aagaagagtc tgggcaagct gatcggcaaa
      781 atctttcgcg ccgacggcaa gaagcttgaa attacaggtg aagggatttc ggctgctcgc
      841 cgcggtacta agctaaagat caagaccgac agcggtgaaa tttcagcaat agtatctgag
      901 accctgcatt cgaagataaa ggcgaatgca gctaaggcag gcgcgagcaa aggcgatgcg
      961 gtctatctgg aaaagtgatg accggtttcc tcaagcggca ggctgacatg gtcaggcaat
     1021 gggtgcggtc gcaaccgcgc ctgcttgcct ggggtgagcg tacactgccc gaactggaaa
     1081 aatttgtcat cgcgggccca acactggtcg gctttgtcct tggcctcgtc aggtaccagc
     1141 caggcactgt gcacttcgaa accggcttcc agtcgctcgt catattcctg gcctacctgg
     1201 cagcgctcac agtcctgcag atggcagata gcctgctttt cagcagctgg cccatcgtgc
     1261 tcggtgtcgc gcgctcactg ctggcggtag tctaccttgc tgtcactatt cgccagtttg
     1321 tacaatggcg cacaggtact ccgcagattt ttcccgcggt gcagcacata cgggagcgcc
     1381 tgcgccctgt aaccggcgat gctgcctgat accctggagc gggacagcgc gaggcatcgc
     1441 ctgcgcaaga tcccggcagc ttttcgaaag gaatataaag acatactgca tgaagtcggc
     1501 agtctgatcg ccgaatactc cctcattgag cagggtgacc gggttatggt cgcggtttcg
     1561 ggcggcaaag attcgcacgc gctgctgcat attctgcttg atctgcaggc gaaggctccc
     1621 atcgactttt cggtggttgc ctacacgctg gaccagaagc aaccgggctt cgatgcatcg
     1681 tcgctcattg cttattacga agaaattggc gtcgagtatg tgctgggtga gcgggatacc
     1741 tattctattg tcacggaaaa aattcccgcg gggcagacat tctgttcgct ctgttcgcgc
     1801 ctgcgccgcg gaattctcta cgacgaagcg cagcgcctga aggcaaacaa gatagcgctc
     1861 ggtcaccatg ccaacgatgc gctcgaaacc ctgctgatga atctcttcta cagcggcaag
     1921 ctcgcggcga tgcccccgcg tcttatcagc gacgatggac gcaatgtcgt gatccggcca
     1981 ctgctgcgcg tcaccgaaga tgcgctggct gccttggcgg aacagctgaa ttttcccatt
     2041 ctgccgtgca acctgtgcaa caaccaggaa ggcctgcagc gcgcgcgtat caagcagctc
     2101 atcgccgaag aacaaaaacg taatccgaac ctgttgcact cagcgcgggg ggctatggcg
     2161 aatgtgaagc catcacacct gtgggatctt tccctctcca cggccaggga cgccgaataa
     2221 ctctcatggc cgccgcaaaa gaaaaaccgg gcaagattgt gccgctcttt gcggggttgc
     2281 gccgcaagcg cgccaaaacg caggcagctt cacagcagag cggcacgcag ctattccggc
     2341 aggttgttac ctatgtccgc gaccgggcga aggaacgcca cattggctgg agcctgattc
     2401 cggcactggt cactttgcgt attgtcgcgg cggaaaatct gcgggcaatt cgcgcggctt
     2461 ttgaagaagg gatctcggca gagcgccctg tgcatcccga tctgctggga gagtttctgg
     2521 agctgcgccg gtattttact gctgcgattg tctacaaggc ggcggaaaac tggaagcggc
     2581 agcatgcaga caagaagagg acgcgccaac tcgacgagct gctcgaagaa ctacaattcc
     2641 tgagcgagaa ccagtcctgg gactgggtga gtttccatga aaccgaattc gaattctttc
     2701 tgcagatcgc ctacctgtca cgtttcacca ccgccaaatt tgtcctgaac cgtatccgcc
     2761 gcgactacct cgactcagtg ctcgcgtata acaagcattt ctggaatatg gaaaccgcgg
     2821 tgtccgttta ccgcacggta gtgaatctga ttcgcgaatc caagcccgca gaagccgagg
     2881 gcgaactgct gcagttcttt acgcagaatg acctggcgat tctgcaatat tatacgagcc
     2941 aggtcgcgca gcgtggtttt gagctcgcaa cggcgccgca gcgtgaccgt gagcgccccg
     3001 tctacgcctc gaatgttccc gtgctgggtg acgtggaaat tggccgggca gtgcgttacg
     3061 cgctggctga agacaaggct tctgcggaca ttacgacgca ggcgattttt accgagagtg
     3121 cacggtcgac gggccgtatc ttcgtcaaga ctgactgcgt tgtctgtggt attcgcgtcg
     3181 cagaacagac ttttcgttac tttgacacgg gcatggtcat caggaccgct gccgcagatg
     3241 gggatttact gaaggcaggc agcactgtcc tcgagtttga aggcgatatt cgcgcagtgc
     3301 tgcgcgcaga acgagtcgcg ctcaattttc tggccttcat gagcagcatc gcgacccgca
     3361 cgcagcagat ttcgcgtgcg gcagcagtct acggcgtcaa agtactcgac acccgcaaga
     3421 ccgtgcccat gctgcgcgcg atctcgaaat acgccgttgt gcgcggtggt ggctacaacc
     3481 acaggcatga cctttcagaa atggggctca tcaaagacaa ccatatcgca caggcgggtt
     3541 ccgtcacggc ggcaatcctg gctttcaagc gccacgcacc gtttacaccg ctggaggtcg
     3601 aggtcgatac gctggaacag ttgcaggaag ctttgccgct taaacccgaa atgatactgc
     3661 tcgacaacat ggacaatgcc accatgaagc aggcaatgaa actgttccgt gaggcgaaca
     3721 cggccatggg gaccaagatc acagcagaag cctcgggtgg att
//
LOCUS       DAOQJF010000322         3762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15170_length_3762_cov_5.811708, whole genome shotgun sequence.
ACCESSION   DAOQJF010000322 DAOQJF010000000
VERSION     DAOQJF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15170_length_3762_cov_5.811708"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="mreB"
                     /locus_tag="PLF85_14190"
     CDS             complement(<1..546)
                     /gene="mreB"
                     /locus_tag="PLF85_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HNE20869.1"
                     /translation="MIFDWFHSLFSNDMGIDLGTANTLVYVKGQGIVLSEPSVVAIQA
                     STGKVLAVGQEAKRMLGRTPGEIVAIRPMKDGVIADFETVEKMIRYFINKVHNRATLV
                     KPRIVIGVPSGITEVERRAVRESAEQAGARDIFLIDEAFAAALGAKIPVHEPAGNMII
                     DIGGGTTEIAVISLGGMVIADS"
     gene            complement(643..1881)
                     /locus_tag="PLF85_14195"
     CDS             complement(643..1881)
                     /locus_tag="PLF85_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNE20870.1"
                     /translation="MKPDKEIMTPFEQTNYYLRKAFALAKLGEALEQALRTPVRELAC
                     RLTIERDNGELMNFSAFRVQHNNARGPFKGGIRYHHEVDVEEVRSLASLMTWKTAVVD
                     IPFGGAKGGIACKPAELSDREKERLTRAFVRQLEPVIGPDLDVPAPDVNTSAREMGWF
                     VDEFERLRGYAPGVVTGKPLALGGSLGRDAATGRGTLFAIREILRLRGEGSIAGKTFA
                     VQGFGNVGMWAARLIAEAGGKVIAVSNSKGGIVNQAGLDIAGIVKSYHGKLEGDAISN
                     EALLELECDVLIPSALGGVITAENAGRVRAKIIAEAANHPVTPEADELLRKKGATLIP
                     DILCNAGGVTVSYFEWVQNAQRLAWGEDQVNRALEDKMVSATSAVFARAQTAQCDLRT
                     AAFLLAVERVAEATRLRGLG"
     gene            complement(1871..2794)
                     /locus_tag="PLF85_14200"
     CDS             complement(1871..2794)
                     /locus_tag="PLF85_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNE20871.1"
                     /translation="MNAFDVAVWGGLFLYFLFKLVRSVRVIPPRVAAIVERLGKYHST
                     LLSGFHVLIPFIDKVTFIQDLKEEAITVPPQDCFTHDNVKVEVDGVIYMMVTDPVLAS
                     YGVTDYRQASIQLAQTTIRSVMGTLDLDRTFEEREVINQKIVGVLAEVGQTWGIQVLR
                     YEIKNIVPPVSVKNAMERQMTAERERRAQIARSEGDMQANINNSEGLKTEMINKSEGE
                     KQRRINEAEGRAREIEALAEATAHAIEAVAEAVREPGGNQAMALRLTEAYLGKLSGIA
                     RKDATVILPADLTRLDEQLAALGLGAGKNET"
     gene            complement(2791..3738)
                     /locus_tag="PLF85_14205"
     CDS             complement(2791..3738)
                     /locus_tag="PLF85_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNE20872.1"
                     /translation="MDNFFTIVILAGFVLVLRMFRVVPMRVLAVKERLGKFKGVLKPG
                     FHFIVPFIDRIAYMHDSREQVIDIPKQRCITRDNVEVDVDGVVYLKVADAQKASYGIT
                     NYHAAVISLAQTTMRSEIGKLNLDDTFREREKVNDKIVQEIDKASEPWGIKFIRYEIR
                     TIEPSANMLNTMEKQMEAERQKRADITLAQGEKQARINVSEGEKQAAINVSEGEKLKR
                     INEADGRSAEIRLVADATARGLRRIAEAIGKPGGASAVKMRIVEQFLEEFGKVMAHSK
                     ISVVPARIAELQGYFQGLSTLNQATGPSQPQQPQQGRNK"
BASE COUNT          752 a         1131 c         1046 g          833 t
ORIGIN      
        1 actgtcggca atcaccatac caccgagcga gataaccgcg atttcggtcg taccaccgcc
       61 gatatcgatg atcatgttac cggccggctc gtgcacggga attttggcgc caagcgcagc
      121 ggcaaaagct tcgtcgataa ggaagatgtc gcgtgcaccc gcctgctcgg ccgattcacg
      181 taccgcacgg cgctccacct cggtgatgcc cgagggcacg ccgatcacga tgcgcggctt
      241 caccagcgtt gcccggttat ggactttatt gataaaatag cggatcatct tttcgacggt
      301 ttcgaagtcg gcgatcacac cgtctttcat cgggcgaatc gccacgatct cacccggggt
      361 acggccgagc atgcgcttgg cttcctggcc caccgcgagc accttaccgg tcgaagcctg
      421 gatagcgacc accgatggct ctgagagcac gataccctgg cctttgacgt acaccagcgt
      481 gttcgcggta cccaggtcga tgcccatgtc gttagaaaaa agggagtgaa accaatcaaa
      541 tatcataagt gccttgtttc ggctaagtgc acccctgaaa caaggtgaca accaaaactt
      601 atggaggaac tccgcaggac gctggggcgg cctgcggggc gtttagccta agccgcgcag
      661 gcgggtggct tcggcgaccc gctcgacggc cagcagaaag gctgctgtac gcaggtcgca
      721 ctgtgcagtc tgtgcccgtg caaacacggc agaggtcgcg ctgaccatct tgtcttcgag
      781 cgcccggttc acctggtctt cgccccatgc caggcgctgt gcattctgta cccattcaaa
      841 gtacgagacc gttacaccac cggcattgca gaggatgtcg ggaatcagcg tcgcgccttt
      901 cttgcgcagc agttcatcgg cttcaggcgt caccggatgg ttggcggctt cggcgataat
      961 ctttgccctg acgcggccgg cgttttccgc cgtgataact ccaccgagcg cagaaggaat
     1021 aagcacatcg cactcaagct cgagcagagc ctcgttgctg atggcatcac cctcgagttt
     1081 accgtggtac gacttcacga tgcccgcgat atcgagcccg gcctgattga ctatgccgcc
     1141 cttgctatta gaaactgcga tcaccttgcc gccggcttca gcgatcaggc gggcggccca
     1201 catgccgacg ttgccgaacc cctgcacggc aaaggttttt ccggcaatag aaccttcgcc
     1261 cctgagacga aggatttcgc gaatcgcaaa aagggtgccc cgccctgtgg cggcgtcgcg
     1321 tcccagcgaa ccaccgagcg caagcggctt gccggtgaca actcccggcg cgtaaccgcg
     1381 caggcgttca aactcgtcga caaaccaacc catctcacgc gctgaggtat tcacatcggg
     1441 tgcagggaca tcgagatcgg ggccgatgac aggctcgagc tgccgcacaa aggcacgcgt
     1501 gaggcgctct ttctcgcggt cgctgagctc agccggcttg caggcaatac cgcccttcgc
     1561 tccgccaaac gggatatcga cgactgcggt cttccaggtc atcagcgatg ccagcgaacg
     1621 gacttcttcg acgtcgactt catgatggta acgaatgccc cccttgaacg ggccgcgcgc
     1681 gttgttgtgc tgcacgcgaa aggcggaaaa gttcatgagc tcgccgttat cgcgctcaat
     1741 ggttagccgg caggcaagtt cgcgcaccgg cgtgcgtaat gcctgttcaa gcgcctcgcc
     1801 gagctttgcg agggcaaatg ccttgcgcag gtagtagttt gtctgctcaa acggggtcat
     1861 tatttcttta tcaggtttca ttcttaccag cgcccagtcc aagggccgca agctgttcgt
     1921 cgagccgggt gagatcagcc ggcagaatta cggtggcgtc tttgcgcgcg atgcccgaaa
     1981 gtttgccaag gtatgcctcg gtgaggcgga gcgccatcgc ctggttgccg cctggttcgc
     2041 gcactgcttc ggcaaccgct tcaatcgcgt gcgctgtcgc ttcggccagc gcttcaattt
     2101 cccgcgcgcg gccttcggcc tcgttgatac ggcgctgttt ttcgccctcg gacttgttga
     2161 tcatctcggt tttcagacct tcggagttat tgatattcgc ctgcatgtcg ccctcagaac
     2221 gcgcaatctg cgcgcggcgt tcacgctcgg cagtcatctg ccgctccatc gcattcttca
     2281 ccgagactgg cggtacgatg ttcttgatct cgtagcgcag cacctggata ccccaggtct
     2341 ggcccacctc tgcaagcacg ccgacaatct tctggttaat cacctcgcgt tcttcgaagg
     2401 tgcggtcgag atcgagagtt cccatgacag aacggatggt tgtctgcgcg agctgtatcg
     2461 acgcctgccg gtaatcggtc acgccgtagc tcgcgagcac ggggtccgtt accatcatgt
     2521 agataactcc atcgacctca accttgacgt tgtcgtgcgt gaaacagtcc tgcggtggca
     2581 ctgtgatcgc ttcttcttta agatcctgga taaaggtcac cttgtcaatg aacggtatca
     2641 gcacgtgaaa gccgctgagc agtgtgctgt ggtatttacc cagccgctcg acaatcgctg
     2701 cgacgcgggg tggaatgacc cggacggagc gtaccagctt gaagagaaag tagagaaaaa
     2761 ggccgcccca gacggctaca tcaaatgcgt tcatttattc ctcccctgtt gtggctgttg
     2821 cggctgcgac ggcccggtcg cctggttgag cgtactcagg ccctgaaaat acccctgtag
     2881 ttcggcaata cgcgccggta cgacagaaat tttggaatga gccatgacct tgccgaattc
     2941 ttcgagaaac tgttccacaa tgcgcatttt gaccgcagaa gctccccccg gtttgccgat
     3001 agcttcagcg atgcgacgca gaccccgcgc cgtagcatcg gctacaagcc tgatctcggc
     3061 gctgcgcccg tcagcttcgt tgatgcgctt cagcttctcc ccttcggaaa cattgatcgc
     3121 tgcctgcttc tcgccctcag aaacgttgat acgcgcctgc ttttcaccct gcgcgagcgt
     3181 gatatcagca cgcttctggc gttcggcttc catctgcttt tccatggtgt tcagcatatt
     3241 ggctgatggt tcgatagtac gaatttcgta gcggataaat tttatgcccc agggctcaga
     3301 ggccttgtcg atttcctgca cgatcttgtc attgaccttc tcgcgttcgc ggaatgtatc
     3361 gtcgaggttc agtttaccga tttctgagcg catagtcgtc tgcgccagcg agatgacagc
     3421 cgcgtggtag ttggtgatac cgtaacttgc cttctgtgcg tcggcgacct tcaggtacac
     3481 aacgccgtcg acatcgacct ctacgttatc gcgcgtgata cagcgctgct tgggaatatc
     3541 gatcacctgc tcgcgcgaat cgtgcatgta ggctatgcga tcaataaacg ggacgataaa
     3601 gtgaaaaccg ggtttcagca cgcccttgaa tttaccgagg cgctctttga ctgcaagcac
     3661 gcgcatcggc acaacccgaa acatccgcag gacgagcaca aagcctgcga gtatgacaat
     3721 agtgaagaaa ttatccatgc gtctaaacgc agctcgtaat gt
//
LOCUS       DAOQJF010000323         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15183_length_3760_cov_4.991093, whole genome shotgun sequence.
ACCESSION   DAOQJF010000323 DAOQJF010000000
VERSION     DAOQJF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15183_length_3760_cov_4.991093"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /gene="tyrS"
                     /locus_tag="PLF85_14210"
     CDS             complement(<1..459)
                     /gene="tyrS"
                     /locus_tag="PLF85_14210"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNE20873.1"
                     /translation="MAAPAEKVELPAELEAQFQELTRGIEEVLPVHEFRKKLLDAHNK
                     KIPLRVKAGFDPTAPDLHLGHTVLIQKLRQFQKFGHHVLFLIGDYTAMIGDPTGKSET
                     RKVLSRAEVEANAKTYKEQVFKILDPQKTEIVFNSKWLNELKLEDMLHLTG"
     gene            complement(498..1616)
                     /locus_tag="PLF85_14215"
     CDS             complement(498..1616)
                     /locus_tag="PLF85_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20874.1"
                     /translation="MKKIFTFTFLSANLCAGLWAQSLPRVLILDFKNKSGNASLGYLE
                     GSITEAVSIEMKKRFTFAETPAEQWKAVAGKNYFFESDLSTDSAAVNLGLLTSQDVVV
                     TGNIVAGKNSSQVLVVVGIFDIGQKKKIEELSLPLALSANMFADIEKIAVKVSDTAAG
                     ILPNKDEWARKGLGDFTTKQRQHVLFTCSLGVLPYTGNRTAELGESSVVSAESFDLKV
                     NLQLHYELDQVWKQYLFVWGGGAIEFGSKKFSTGAGGTVAGSLFGWEFAGGAGVRLVE
                     RVRWRLSLITGLGLYMQSVKYDYSSDRVFALSSATLGLESGKSASAMAVTTPIGATFS
                     YRLTPDVSVVAGAVVKGRFFQNSHGFSTYFMLGAGYDF"
     gene            complement(1649..>3760)
                     /locus_tag="PLF85_14220"
     CDS             complement(1649..>3760)
                     /locus_tag="PLF85_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNE20875.1"
                     /translation="EMRVYSRALTAAEIRKLATKVPSGLVAQYPLDDNITSGSVVDNS
                     GNGNNALISGNPLPGPDRFGRVASAYVFDGTGDLLRNTAANSLSGSALPRTICAWFRR
                     TNMTGSVIFVDTGASTATVGNMYRIAGLAAGQAKSDFYTTANSDSTSLYSINNWSHVC
                     GVYNGSTNTVYLNGEAGPAVAYTATASANAISLGGTMDFTNLLIGSLDDVQVYNRALS
                     AGEVKALATELDRGLVAYFPLDEATSATVSDYSGSGFTLAGSGAAGPQNTPQSTSDRF
                     GNAQSAMNFDGNDDMLSGSAASILPSGAATRTGCAWSLTTVDPGNGNAQGLMAYGTNS
                     ANQTFGIYPFHSVSDTFMLGAYTSSSTFFYAQVPGNGWPMNQWAHYCIIVTGATSVEY
                     YMNGIKFNQSGTSGSPSVNTVLSLFRVGTSMSGASRFTGKIDDVRVYNRALSLAEIRE
                     LSGFAPGQIDNNTLWLDAGRAVFSDAGTTLATNAQSVQQWNDVSMDNGTQNANNMTQT
                     TATNRPTWTSSVATLGNLPAINFDGTNDFLTAGSVLGSKLFNTNESTNFVVLRQNSSA
                     GTNVLYTWGPNSNRYLNAIVGTTQLQFGQGDLSAGGQLLANQPAGWAGNAQLLEMTRL
                     TSNAAYINVNSVNMLTSTITDPIDNSGSNTFNISHTSNQFNGDIAEIIIYKTGLTQSE
                     REKITCYLSKKYAIAASVVCD"
BASE COUNT          815 a         1053 c         1018 g          874 t
ORIGIN      
        1 accggtcagg tgcagcatgt cttcgagctt cagctcattc agccacttcg agttgaagac
       61 aatctccgtt ttttgcggat cgagaatttt gaagacctgt tctttatagg ttttggcatt
      121 ggcctcaacc tcggcgcgcg agagcacctt gcgcgtctca gacttgcccg tcgggtcgcc
      181 gatcatcgcg gtatagtcgc cgataagaaa gagtacgtgg tggccgaact tctggaactg
      241 gcgcagcttc tgtatcagca ccgtgtggcc gaggtgcagg tcgggcgcgg tcgggtcaaa
      301 gcccgccttc acgcgaagcg gaatcttctt gttatgtgca tcgagcagct tcttgcgaaa
      361 ctcgtgtacc ggcagtactt cctcgatgcc gcgcgtgagt tcctggaatt gcgcctcgag
      421 ctcggcgggt agttcaacct tttctgcggg tgctgccatt tgttagagtc tattcacgag
      481 acaggccggc aagccggcta gaaatcgtag cccgcaccga gcatgaagta ggtgctgaag
      541 ccgtggctgt tctggaagaa tcggcctttc acaacggcac ccgcaactac tgaaacatcg
      601 ggggtgaggc ggtagctgaa agtggcgccg attggtgtgg tgactgccat cgcagaggca
      661 gatttgccac tttcaaggcc aagcgttgcc gagctcaggg caaacaccct gtcagaactg
      721 tagtcatatt tcacgctctg catatagagg ccgaggcctg tgatcagcga caagcgccag
      781 cgcacccgct cgaccagacg cacccccgcg ccaccggcaa attcccagcc aaagagagaa
      841 ccggcgactg tcccccctgc acctgtgctg aattttttcg agccaaactc aattgcgcca
      901 ccgccccaaa cgaacaggta ctgcttccag acctggtcga gctcatagtg cagctgcagg
      961 ttcaccttca aatcaaaact ttctgccgac accaccgagc tttctccaag ctccgctgtc
     1021 ctgttgcctg tgtacggcaa gacccctaac gaacaagtaa acaacacatg ctggcgctgt
     1081 ttggtcgtaa agtcgccgag gcctttgcgc gcccattcgt ctttgttagg caggattcca
     1141 gccgccgtgt ctgagacctt gaccgcgatc ttttcaatat ccgcaaacat gttggcagag
     1201 agcgcgagcg gcaggctcag ttcttcaatc ttcttctttt gcccaatatc gaagatgcct
     1261 acaacaacca gaacctggct cgagtttttg cctgcgacga tattccccgt aacgaccaca
     1321 tcctgactcg tcaagagccc gaggttcacc gccgcgctgt cggttgaaag gtcgctttca
     1381 aagaaataat tctttccggc aaccgccttc cattgctccg ccggtgtctc ggcaaaggtg
     1441 aaccgctttt tcatctcaat gctgaccgct tcggtaatcg aaccttcgag gtagccaagc
     1501 gatgcattgc cggatttatt cttgaaatcc agaatcagca cgcggggcag agactgagcc
     1561 caaagcccgg cgcaaaggtt ggcagagagg aaagtgaagg tgaatatttt tttcacgtca
     1621 tccccggccg cctgtgcttt cgcgccgctc agtcacagac aacactcgct gcaatcgcgt
     1681 atttcttact caggtagcac gtgatctttt ctctctcgct ttgggtgagc cccgtcttgt
     1741 agatgataat ttcggcgatg tctccgttaa attggttcga cgtgtgactg atgttgaatg
     1801 tgttcgaacc cgaattgtca atcgggtcag taattgtcga tgtaagcatg ttgacgctgt
     1861 ttacatttat gtatgcggca ttggaggtta gccgggtcat ttcgagcaac tgcgcattgc
     1921 cggcccaacc ggcaggctga ttcgcaagca actgccctcc agccgagagg tcaccctgcc
     1981 caaactgcaa ttgtgtggta ccgacaatgg cattgaggta gcggttcgaa tttggccccc
     2041 aggtgtacaa aacgtttgtc cctgcagagc tattctgtcg cagcacgaca aaattggtcg
     2101 attcgttggt attaaaaagc tttgacccca gcacagagcc cgccgtcaga aagtcgtttg
     2161 taccatcaaa attaatggcc ggaagattcc ccaaagtcgc cacactgctg gtccatgtcg
     2221 gtctattcgt ggcggttgtt tgagtcatat tgttcgcatt ctgcgtcccg ttatccatgc
     2281 tcacatcgtt ccactgctgc acagattgtg cgtttgtcgc gagagttgtt ccagcatcac
     2341 tgaacacagc tcgcccggca tcaagccaca gcgtgttatt gtcgatctgc cccggcgcga
     2401 agcccgacag ttcgcggatt tcggcgagcg aaagtgcgcg gttatagaca cgcacatcgt
     2461 cgattttccc ggtaaacctg ctcgcgccgc tcatcgaggt gccgacacga aagagcgaca
     2521 atacggtgtt gacactgggc gaacctgatg taccggattg attgaatttg atgccattca
     2581 tatagtactc cacagacgtt gcaccggtaa caatgatgca ataatgcgcc cactggttca
     2641 tcggccatcc gttaccgggc acctgcgcat agaaaaaagt cgatgagctg gtataagctc
     2701 ccagcatgaa agtatcgctg acgctgtgga aagggtaaat tccgaatgtc tggttcgcgc
     2761 tattggtgcc atacgccatg agtccctgcg cattaccgtt gccggggtcg acggtggtca
     2821 gactccaggc acagcctgta cgcgtcgccg ccccggaagg caagatcgat gctgcactgc
     2881 ccgagagcat gtcatcattg ccgtcaaagt tcatggcgct ctgagcgtta ccgaaccggt
     2941 cgctggtcga ttgcggagtg ttttgggggc ccgccgcacc ggagccggcc agcgtaaatc
     3001 cgctgccgct atagtcgctt acggttgcgc tggtggcctc atccagagga aaatacgcca
     3061 caagcccgcg atccagctcg gtcgcaagtg ccttgacctc gccggcactg agggcgcggt
     3121 tgtagacctg tacatcgtca agcgacccga tgaggagatt cgtaaaatcc atggtcccac
     3181 caagcgaaat ggcgttggct gatgctgtgg ccgtatacgc aacagcgggc ccggcttcgc
     3241 cgttgaggta aacggtattt gtggatccgt tatatacgcc gcagacatgc gaccaattgt
     3301 tgatcgagta tagactcgtt gaatcggagt tggccgtagt gtaaaaatca gatttagcct
     3361 ggcctgcggc gagcccggca atgcggtaca tgttgccgac cgtggcagtc gacgccccgg
     3421 tgtctacgaa gatgaccgac cccgtcatat tcgtgcgtct gaaccaggca cagatggtgc
     3481 gcggtaaggc agatcccgaa agtgaatttg ccgctgtatt gcgaagcaaa tcgccggtgc
     3541 cgtcaaacac gtacgctgat gccacccggc caaaacgatc gggaccaggt aatggattgc
     3601 cactaatcaa ggcattgttg ccgttgcccg aattatctac gacagagccg ctggtgatat
     3661 tatcgtctag cggatactgc gcgaccaacc cgcttggtac ctttgtcgcc agcttcctga
     3721 tttcggccgc cgtgagcgcg cgggagtaga cgcgcatttc
//
LOCUS       DAOQJF010000324         3756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15209_length_3756_cov_3.976493, whole genome shotgun sequence.
ACCESSION   DAOQJF010000324 DAOQJF010000000
VERSION     DAOQJF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15209_length_3756_cov_3.976493"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="PLF85_14225"
     CDS             <1..1555
                     /locus_tag="PLF85_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE20876.1"
                     /translation="QSTDDKPLASAFVTKSPIVVYEGPDTKAKVVSTLPIGVQVGIFA
                     SRVPDKAQPDQAFWYKVRYAPLSASLAPSSLPVEGYVSEREEVLRNNLMVFSSASRVT
                     YRKETAPGKFEDVEEAQSVIATTAVNFRQSPALNAPILRTLKNGEVLALEEQSGKTYL
                     VDKKNGYWYKLRSQTGEVGYAFGGFMVPGPASEIQRLEQAGFQFQPGWIIPVGNKGIV
                     YAGPVGNTRIQITEDAYYIPEAWRTSKGVLEKEFYIKADGLTTKGEPQRYRITLRQQT
                     DYEVFQEHYFYVDKSAVKFVRDYYSVSEKQPHEFDAELAPLVNEFVGGDLNLQCSSVQ
                     EFSGGPAEAPRQFKVISASIGASQNGTDESSRCALNDRQTLVAEVINDKLLLSRSEQG
                     DFRDLDGDQIPEIVAEGGGRGYRTLDIYSIKQGRTTQLANLSADAEGSTTFGDFSIDD
                     KGFLHIGAPYECRDEDLEAEAKKRCSQKLASARSTAKFLKLDPKLPLFPFKGKLEGDK
                     FVQVQNDDG"
     gene            complement(1596..2762)
                     /locus_tag="PLF85_14230"
     CDS             complement(1596..2762)
                     /locus_tag="PLF85_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNE20877.1"
                     /translation="MLRRQNQGLFCCLVALELVSCSKPATETTPLFSKGVYPVVFKAE
                     DFPPHPKTGKAMQSSEDCAGCHATVYRNWQKSRHRVALTNELYKESHAREPSPWCVNC
                     HAPLRLLGSEKTPYRGEEGISCLACHARGGEILTGALPVPAKPALKGKLAHSYRIDAQ
                     FKDERLCENCHEFDFPTAASAMSEGKDFHYTDQPMQSTVSEYRASAFYGKVTCQGCHL
                     FRDTADSHTFPGGHALERLKKDLRVEAVRESEGSLTLRIYAQGIGHAFPTGDLFRTLR
                     VLLYDKTVNEQQEVELRHFFENLPAAQGRPQAPLKLRVADETLPPPKTDYLSVREYAL
                     PWPAASRQVRAELYIDYLSGQNTRTTHLPARFTRPLIKRQDFPLKSKPATDSKG"
     gene            2870..3652
                     /locus_tag="PLF85_14235"
     CDS             2870..3652
                     /locus_tag="PLF85_14235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE20878.1"
                     /translation="MQKPLALITGASTGIGYEMAKILATKGFDLVVVARNAKKLKALH
                     DELAGQAKVTPLALDLSQPKAAQALYQKTSKAKLKIDLLINNAGYGSSGAFVSLDLKK
                     ELGQIDLNIRALTELCHLYGADMAKRKSGKILNVASTAAFQPGPWMSVYYATKSYVLH
                     FSEGIAEELKKSGVQVSVLCPGPTVTEFADTAGMDKSLLFSSKLMKPATANYVAQYGV
                     EKLLKGKLIIIPGFMNRAMAFGNRFSPRFLVRKIAGYLNGGG"
BASE COUNT          843 a         1002 c         1089 g          822 t
ORIGIN      
        1 acaatcaaca gatgataagc cgcttgcgag tgcctttgtc accaagagcc ccattgttgt
       61 ttatgaaggc cccgatacga aggcgaaggt cgtgtccaca ctgcccatcg gggttcaggt
      121 gggtattttc gcatcgcgtg tgcccgacaa ggcacaaccc gatcaggcct tctggtacaa
      181 ggtccggtat gcgccgctga gtgccagcct tgctccttcg tcgctgccgg ttgaagggta
      241 cgtttcagag cgagaagagg tattgcgcaa caacctcatg gttttttctt ctgcctccag
      301 ggtgacctac cgcaaagaaa cagcgccagg caagtttgaa gatgtcgaag aagcccagtc
      361 ggtgatcgcg acgaccgcgg tgaactttcg tcaatcacct gcgctcaatg cacctatcct
      421 gcgcaccctg aagaatggcg aagttctggc actcgaagaa caatcaggca agacgtacct
      481 ggttgataaa aagaacggtt attggtacaa gctgcgctcg cagacaggtg aagtgggtta
      541 tgcctttggt ggcttcatgg tacctggccc ggctagtgaa atccagcgcc tcgaacaggc
      601 gggattccag ttccagccgg gctggatcat ccctgtcggt aacaagggca tagtttacgc
      661 gggccccgtt ggtaacaccc gcatccagat taccgaagat gcgtactaca tacctgaagc
      721 atggcgcaca agcaaagggg tgctcgaaaa agagttttac attaaggcag atggcctgac
      781 aacgaagggc gagccgcagc gctaccgcat cacgctgcgg cagcagaccg actacgaagt
      841 tttccaggag cactatttct acgtcgataa gagcgcggtg aaattcgtgc gcgactacta
      901 cagcgtttca gaaaaacaac cgcacgagtt cgacgccgag ctcgcgccgc tcgtgaacga
      961 atttgtcggc ggtgatctga acctgcagtg cagcagtgtg caggaattct ccggtggccc
     1021 ggctgaagct ccccggcagt ttaaggtcat cagcgcttcc atcggtgcat cacagaacgg
     1081 cacggatgaa agctcgcgtt gcgcattgaa cgaccggcag accctggttg ccgaggttat
     1141 taacgataag ctgcttctaa gcagaagcga acagggagat ttccgcgacc tggacggtga
     1201 ccaaataccc gagatcgtag cagagggtgg cggacgtggt tacagaacgt tggatatcta
     1261 ctccattaag caggggcgca caacgcagct ggccaatctc agcgccgacg ctgaaggttc
     1321 aactacgttc ggagattttt ctatcgacga caaaggcttt ctgcatattg gtgcacctta
     1381 cgaatgccgg gacgaagatc tggaggcaga ggctaaaaaa cgttgttcgc agaagctcgc
     1441 cagcgcacgc agtactgcca aatttctcaa actggacccc aaattgccgt tgttcccttt
     1501 caagggtaag ctcgaaggcg acaagttcgt ccaggtgcag aacgacgacg gttgattttt
     1561 cggctgctcg cgagcaagaa ttctgaaccg gctggttagc ccttgctgtc ggttgccggt
     1621 ttggatttga gtggaaaatc ctgccgcttg atcagcggcc gggtgaacct ggcgggcagg
     1681 tgcgtcgtgc gcgtgttctg acctgacaaa taatcgatat aaagttctgc gcgcacctgg
     1741 cgtgaagctg caggccatgg cagggcgtac tcgcgcaccg agaggtagtc tgtcttcggc
     1801 ggcggcaggg tttcgtcggc gacgcgcaat ttgagcgggg cttgcgggcg gccctgcgca
     1861 gccggcaaat tttcaaagaa atgccttagc tccacctctt gctgttcatt cacagtcttg
     1921 tcatagagta acacccgcag ggtgcggaaa agatcgcccg tcggaaaagc atgcccgata
     1981 ccctgtgcat agatgcggag cgtcagcgaa ccttcactct cgcgtacagc ctcaacgcgc
     2041 aggtcttttt tcagccgctc gagcgcatgc ccgccgggaa aagtgtgcga atcggccgta
     2101 tcgcgaaaga gatggcaacc ctggcaggtt accttgccgt aaaacgccga ggcgcggtat
     2161 tcagaaacag tcgattgcat gggctggtcg gtgtagtgga aatctttgcc ttcggacatg
     2221 gccgacgccg cagtgggaaa atcgaactcg tggcaattct cgcacaggcg ttcgtccttg
     2281 aactgcgcat cgatgcggta gctgtgagcc agctttccct tgagcgctgg cttggcggga
     2341 acaggcagcg ctccggtgag tatctcgcca ccgcgcgcgt ggcaggccaa acacgaaatt
     2401 ccttcttcac cccggtaggg agttttttca ctgccgagta agcgtagcgg ggcatggcag
     2461 ttgacgcacc agggcgaagg ttcgcgtgcg tggctctctt tgtagagttc gttggtgagc
     2521 gcaacccggt gccggctttt ttgccagtta cggtagactg tcgcgtggca gcctgcgcag
     2581 tcttcagaac tctgcatggc tttaccggtt ttcgggtgcg gcggaaaatc ttcggctttg
     2641 aacactacgg gatatacacc cttgctgaaa agcggggttg tttcggttgc cggtttgctg
     2701 caggagacta gctccagcgc gacgaggcag cagaagagac cctggttttg tcgccggagc
     2761 attcgcgaga atcgcccgtg catcccggcg ctgcggcaag cattttcagg ttcgataacc
     2821 aaatgcgaaa agtgctttct ggcaaaggcg gctgacccaa tcaatccgca tgcaaaaacc
     2881 cctcgcgctc atcaccggtg cttccactgg aataggttat gaaatggcca aaattctcgc
     2941 caccaaaggt tttgacctgg tggttgttgc gcgtaacgca aagaagctta aggcgctgca
     3001 cgatgagctt gcggggcagg caaaggtgac gcctttggcg ctcgatcttt cgcaacccaa
     3061 agcagctcag gcactgtacc agaaaacctc taaggcaaag ctaaaaattg acctgctgat
     3121 caacaatgcc ggttacggtt cgagcggcgc ctttgtctcg cttgatctca aaaaggagct
     3181 cggacagatc gacctgaata tccgcgcgct gacagaactc tgccatcttt acggtgccga
     3241 tatggcaaag cgcaagagcg gcaaaatttt gaacgtcgct tcgactgccg cgtttcagcc
     3301 tggcccttgg atgtcggtat actatgcgac caagtcttac gtgctgcact tttctgaagg
     3361 tattgccgaa gaactgaaaa agtccggcgt gcaggtttcg gttctgtgcc cggggccaac
     3421 cgtaaccgaa ttcgccgata cggctggtat ggacaaatcc ctgctcttca gctcaaagct
     3481 gatgaagccg gcaactgcaa attatgtggc gcaatacggg gtggaaaaat tgctcaaagg
     3541 caaactgatt attataccgg gcttcatgaa ccgggcgatg gcgtttggca accggtttag
     3601 cccgcgtttt cttgtacgga agattgcggg ctacctgaac ggcggggggt agcccccagg
     3661 tgcgcgcttt tccgtaacgc cgtcagatct gcatctcggg tatttgcccg tcgacgatca
     3721 ggtggccttc ggttgcgttc ttcacctgct cgacac
//
LOCUS       DAOQJF010000325         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15458_length_3719_cov_3.882915, whole genome shotgun sequence.
ACCESSION   DAOQJF010000325 DAOQJF010000000
VERSION     DAOQJF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15458_length_3719_cov_3.882915"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLF85_14240"
     CDS             complement(<1..861)
                     /locus_tag="PLF85_14240"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNE20879.1"
                     /translation="MADSAEQAYSSDERFERLRTRIGLFVGPLLAVVVFLLPMPGLTP
                     AAHTLAAVLSLVIIWWITEPVPIPVTGLIGLALCVLFGVESSRKVFAAFSDPVIFLFI
                     GSFILAQAMSLHRLDERIAFTILALPGVANHPLSILFVFGALATVLSMWVSNTATTAM
                     LLPIGIGVLRTVREIEIREFPETAKTLPPVEQMKFSSAFVLIAAFGASVGGVATPVGS
                     PPNLIGIGLIDQILGVKISFFQWMRLGLPLALVMFLVVLVVVVLLYPSELKGMSGAAA
                     YIREKKKSLGP"
     gene            complement(891..1892)
                     /locus_tag="PLF85_14245"
     CDS             complement(891..1892)
                     /locus_tag="PLF85_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNE20880.1"
                     /translation="MTQQVLTKKDFASDQTVRWCPGCGDYGILTAVQKTMPELGVAKE
                     DIAFVSGIGCSSRFPYYMNTYGFHTIHGRAPAIASGLKIMRPELSVWMITGDGDALSI
                     GGNHLIHILRRNLDINILLFNNEIYGLTKGQYSPTSARGTVAKSTPEGSIDTPFSPLR
                     LAIGAGASFLARTYDKDMKHMEKTVKSAHLHKGTSFVEVLQNCVIFNDEVYEPIVGKE
                     NRHNTMIFVEHGQKMLFGENNSKGLKLNHGTFEIVSAVDNPDQVAVYNANDPHFLQTF
                     EAVGGKNGIPVPFGVLYQDNRSTYEDDLQAQVEAAVTKKGRGDLRKLLHAAETWEVR"
     gene            complement(1901..>3719)
                     /locus_tag="PLF85_14250"
     CDS             complement(1901..>3719)
                     /locus_tag="PLF85_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNE20881.1"
                     /translation="PGATVRFTGDSGDGMQLVGAQFTQVTGLAGNDVMTFPDFPAEIR
                     APQGTTAGVSGFQVHFGDHHIYTPGDKVDVLVAMNPAALKANLKDLRERGVLLVNSDE
                     FDQKNLEKVDYKSNPLDDDALKQKYRLIAAPITESTRRALAESGLTTKEIDRCKNFFA
                     LGLTYWMFHRDIEKTKEWLNNQFAKKPHLAKANIQVLEAGYHFAETTELFDVSYEITK
                     AEFEPGTYRNITGNSALALGLVAATDLAGIPALYAGYPITPASDILHELAKYKNYNIK
                     TFQTEDEIAACCAAIGASYGGTIGITASSGPGIALKSEAINLAVMTELPLVVVDVQRA
                     GPSTGMPTKNEQTDLFMALFGRNGESPVPVVAASSPADCFDAAIEATRIALKFMTPVF
                     LLSDAYIANSSEPWKIPQVENLPEIDADQIKAGESREGYKVYGRSAETLARRWPVPGT
                     PGFEHRIGGIEKDENGNINYDPENHDKMVKLRQAKIDKIADFIPELEAYPNNKGGLLV
                     LGWGSTYGALREATLRARKQGYDVSHAHLKHLNPFPKNIEKVLRSFDKVLIPELNLGQ
                     LALIIRGKYLVPAYTYNKVRGRPFGIEEMEQVIIETIKK"
BASE COUNT          809 a         1010 c         1071 g          829 t
ORIGIN      
        1 aggcccaagg gatttcttct tttcacgaat ataggccgct gccccgctca tgcccttcag
       61 ttctgaagga tacagcagca cgaccacgac caaaacaaca aggaacatca ccagtgcgag
      121 tggcagcccc agccgcatcc actggaagaa agaaattttt acacccagaa tctggtcgat
      181 gaggccaatg ccgatcaggt ttggtgggct gccgacgggt gttgctaccc cgccgacact
      241 cgctccgaac gccgcgatga gcacgaaggc cgaggaaaac ttcatctgct cgacgggcgg
      301 caacgtcttg gctgtttcag gaaactcgcg gatttcgatt tcgcggaccg tgcggagcac
      361 gccaatgcca atcggcagca gcatcgccgt cgtcgcggtg ttactcaccc acatcgacaa
      421 gacggtagcg agcgcaccaa agacaaacaa gatcgagagc gggtggttgg cgacaccggg
      481 cagcgccaga atcgtgaagg caatacgctc gtcgagcctg tgcaacgaca ttgcctgcgc
      541 cagaataaag ctaccgataa agagaaaaat taccgggtca gaaaatgcag caaagacctt
      601 gcgtgaactt tctacgccga aaagcacaca gagcgcaagg ccaatcaggc ccgttacagg
      661 aatcggcact ggctcggtga tccaccagat gatcacaagc gacagcacgg cagccagcgt
      721 gtgcgccgcc ggtgtcaaac cgggcatcgg cagaagaaag acaacaacgg cgagcaaggg
      781 accgacaaac agcccaatac gggtacgcag gcgttcaaaa cgctcgtcgg aggaatatgc
      841 ctgctctgcc gaatcagcca tcgcctaaaa cagcagagca gccagtctca ttagcgaact
      901 tcccaggttt cggcagcgtg gagcagcttg cgcaggtcgc cacggccttt tttggtgacg
      961 gcggcttcaa cctgtgcctg caggtcgtct tcgtacgtac tgcggttatc ctggtagagc
     1021 acaccgaacg gtacgggaat accgttcttg ccacctacag cctcaaaggt ctgcaaaaag
     1081 tgcgggtcgt tggcattgta gacagctacc tggtcggggt tatcgactgc cgatacaatt
     1141 tcaaacgtac cgtggttgag cttgaggccc ttcgagttgt tttcgccgaa cagcatcttc
     1201 tgcccgtgct cgacaaaaat catggtgttg tggcggttct ctttgccgac aatcggttcg
     1261 tagacttcat cgttgaagat cacgcagttc tgcagcactt ctacaaacga cgtgcccttg
     1321 tgcaggtgtg ccgatttgac cgtcttttcc atgtgcttca tgtctttgtc gtaggtgcgc
     1381 gcaagaaatg aagctcccgc accgatggca aggcgcagcg gtgaaaaggg cgtatcaatg
     1441 gaaccttcgg gagttgattt ggcgacggta cctctcgcac tcgtgggcga gtactgcccc
     1501 ttggtaagac catagatctc gttgttgaag agcaggatat tgatgtcgag attccggcgc
     1561 agaatatgga tcaggtggtt tccgccgatc gagagcgcat cgccgtcgcc ggtaatcatc
     1621 cacaccgaaa gttcgggacg catgattttc agtcccgacg caatcgccgg agcacggccg
     1681 tggattgtat ggaaaccgta tgtgttcatg tagtacggga aacgcgaaga gcagccaatt
     1741 cctgagacga acgcgatgtc ttctttcgcg acgcccagct cgggcatggt tttttgtact
     1801 gctgtcagaa taccgtagtc gccgcagccg gggcaccagc gcactgtctg gtctgaggca
     1861 aaatcttttt tggtcaaaac ttgctgtgtc atgattcctc ttatttcttg atcgtttcga
     1921 tgattacctg ttccatctct tcgatgccaa acgggcgacc acgcactttg ttataggtgt
     1981 acgctggtac caggtacttg ccacggataa tgagcgcgag ctgcccgagg ttgagttcag
     2041 gaatcagcac tttgtcaaac gagcgcagca ccttctcaat gttttttggg aatgggttga
     2101 ggtgtttcag gtgtgcatgc gaaacatcat aaccctgttt gcgcgcgcgc agcgtcgctt
     2161 cgcggagcgc cccgtaggta gaaccccagc cgagtacaag cagcccaccc ttgttgttcg
     2221 ggtacgcttc gagctcggga ataaagtcag cgatcttgtc aatcttcgcc tgacgcagct
     2281 tcaccatctt gtcgtggttt tccggatcgt agttgatgtt accgttttcg tctttttcga
     2341 taccgccgat acggtgctcg aaacccggcg tacccggaac cggccaacgg cgtgccagcg
     2401 tttctgccga acgaccgtag accttgtagc cttcgcgcga ttcaccggcc ttaatctggt
     2461 cagcgtcgat ctcgggcagg ttttctacct gtggaatttt ccacggttca gaactgttgg
     2521 cgatgtaggc gtcggagagc aaaaataccg gcgtcatgaa cttgagcgcg atgcgtgtcg
     2581 cttcgatcgc cgcatcgaag cagtcagccg gtgaagaagc ggccacgact ggcaccgggc
     2641 tttcaccgtt acggccgaac agagccatga agaggtcggt ctgctcgttc ttggtcggca
     2701 tacccgttga aggtcccgcg cgctgcacgt cgacaacaac gagggggagt tcggtcatca
     2761 ccgcgagatt gatcgcttcg gatttcaggg cgataccggg gcctgatgat gccgtgatac
     2821 caatcgtacc gccgtacgaa gcaccgatcg ccgcgcagca ggcagcgatt tcatcttcgg
     2881 tctggaaagt tttgatgttg tagttcttgt atttcgcgag ttcgtgcaga atatccgacg
     2941 caggtgtgat cgggtacccc gcgtagagcg cgggaatgcc cgcaaggtcg gttgccgcaa
     3001 cgagaccaag agccaaagcc gagttaccgg tgatattgcg gtaggtaccc ggctcgaact
     3061 ccgccttagt gatttcgtaa gagacgtcaa agagttcggt ggtctcagca aagtggtagc
     3121 cggcttcgag cacctggatg ttcgctttcg ccaggtgtgg cttcttcgcg aattggttgt
     3181 tgagccattc tttggtcttc tcgatgtcgc ggtggaacat ccagtaagta aggccgagcg
     3241 cgaaaaagtt tttgcagcgg tcgatttctt tggtcgtgag acccgactcg gcaagcgcgc
     3301 gccgtgttga ttcggtgatc ggcgcggcga tcaggcggta cttctgtttc agcgcatcgt
     3361 cgtcgagcgg gttcgacttg taatcgacct tctcgagatt cttctggtca aactcatcgg
     3421 agttcacaag caaaactccg cgctcgcgca ggtctttcag gttcgccttg agcgcggcag
     3481 ggttcattgc cacgagcacg tccaccttat cgcccggtgt atagatgtgg tggtcaccga
     3541 agtgtacctg gaaacccgag acgccggcgg tggtaccctg cggcgcgcga atttcggcgg
     3601 gaaagtccgg aaaggtcatg acgtcgttac cggcaaggcc ggtaacctgc gtaaactgtg
     3661 caccgacgag ctgcatgccg tcgccagagt cgccggtaaa gcgcactgtt gcgccagga
//
LOCUS       DAOQJF010000326         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15475_length_3717_cov_5.341617, whole genome shotgun sequence.
ACCESSION   DAOQJF010000326 DAOQJF010000000
VERSION     DAOQJF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15475_length_3717_cov_5.341617"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="PLF85_14255"
     CDS             complement(<1..1551)
                     /locus_tag="PLF85_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNE20882.1"
                     /translation="MAGQGAVRFPLLPLRDMVIYPGSVTSIFVGREKSMEATRRAMDS
                     DRKIILATQRNAEITIPALSDLYETGVQGEILQLLKMPDNTYKILIEGLHRVRLVDID
                     SSDEKCPVAIAVGADSVAPAGEKEEHLYQKSLTKLVDAFERLNEQQTFLSAEAWRELR
                     AKNDPERLMHAVAESLRIPFVEQQDILGTDSLWERAEKVKAAMENQLELSRLEGQIHD
                     KVRAQLDKMQRQYYLQEQIKAIREEMGETEPDEFESLRKRAKEKVWPDEIRQRCEAEI
                     RKLEKMPPMSAESGVIRNYLDWLLDLPFSESTSDGADIRKAEKILEESHYGLEKVKDR
                     ILEYIAVRQLSSDMRGPILCLAGPPGVGKTSLARSLADSLGRKFVRIALGGVRDEAEI
                     RGHRRTYIGSMPGRIIAALKKVKVRNPVILLDEIDKISSDFRGNPAAALLEVLDPEQN
                     REFNDHYLDLPFDLSQAIFVATANVTHSIPEPLLDRLEIIQLSGYTEMEKIQIANKYL
                     IPRQLKEHG"
     gene            complement(1551..2912)
                     /gene="clpX"
                     /locus_tag="PLF85_14260"
     CDS             complement(1551..2912)
                     /gene="clpX"
                     /locus_tag="PLF85_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNE20883.1"
                     /translation="MAERRSYDSGNHCSFCGKEQAKVNRLIAGPGVHICDECVQLCNG
                     ILADDLEKQATPATVQKLPKPAEIKNILDEYVIGQDAAKRALSVAVYNHYKRLQYDKD
                     QAAKNGGKFMRKHASSDQSAVEIDKSNILLIGPTGSGKTFLAQTLARILQVPCALVDA
                     TALTEAGYVGEDVENILLRLYQNADANIEKAQTGIIYIDEIDKIARKSDNPSITRDVS
                     GEGVQQALLKIIEGTVASVPPVGGRKHPHQDFIQMDTRNILFICGGAFEGLDKIISRR
                     VEKSTLGFDFKAPKRKDQSGEYLRQVTPDDLMKYGLIPEFIGRLPITRSLDNLDEPSM
                     VKILTEPRNALVKQYKKIFELEGIKLTFEDGAIQAIAQKALQRETGARGLRAIVEDAM
                     MDLMFEIPTMIGVDEVIVTREMIESGDKARIITRAETDKERGKKGKQAGEEVYVAPPE
                     KMA"
     gene            complement(2935..3546)
                     /locus_tag="PLF85_14265"
     CDS             complement(2935..3546)
                     /locus_tag="PLF85_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNE20884.1"
                     /translation="MAYAPIVIEQTNRGERHYDIYSRLLKDRIIFLGFPIDDAYANVI
                     IAQLLFLESEDPEKDIYLYINSPGGYVSSGLAIYDTIQLIRPEVRTVCTGQASNIAAL
                     ILAAGAKGKRSALPHSRILLHQPLGGASGQAADIEIQAREVLRIRDTLNGLYQLHTGQ
                     TLEKIEQETERDLYMDGNEAVKWGLIDYVISARGENASKEIAS"
BASE COUNT          758 a         1037 c          970 g          952 t
ORIGIN      
        1 cccgtgctcc ttgagctgcc gcgggatcag gtacttattc gcgatctgga tcttttccat
       61 ctcggtgtaa ccggaaagct gtatgatctc gaggcggtcg agcagaggct ctggtatcga
      121 gtgggtgacg tttgccgtcg cgacaaaaat tgcctgcgaa aggtcaaatg gcaggtcgag
      181 gtaatggtca ttgaactcgc ggttctgttc agggtcaagc acctcgagca gcgccgcggc
      241 cgggttaccg cgaaaatccg aagagatctt gtcgatctca tcgagcagga taacgggatt
      301 gcgcactttt actttcttga gcgcggcgat aatacggcct ggcatcgaac caatataggt
      361 gcgccggtgg ccccgtatct ctgcctcgtc acgcacgccg ccaagcgcaa tacgcacaaa
      421 cttgcgaccc aggctgtcgg caagcgaacg cgcaaggctg gtcttaccga cgcctggtgg
      481 ccccgcgagg cacagaatcg gaccgcgcat atctgaagaa agctgccgca ccgcgatgta
      541 ctcgagaata cggtccttta ctttttcgag accgtaatgg ctctcttcga gtattttttc
      601 cgccttgcgg atatcagcgc cgtcgctggt gctttcgctg aacggcaggt cgagcaacca
      661 gtctaaataa ttgcgtataa cgccggactc tgccgacatg ggcggcatct tttccagttt
      721 gcggatttca gcctcacagc gctgcctgat ctcatcgggc cagactttct ctttggcgcg
      781 cttgcgcagc gactcaaatt catcgggctc ggtttcgccc atctcttcgc gtatggcctt
      841 gatctgctcc tgcaggtagt actggcgctg catcttgtcg agctgcgccc tcaccttgtc
      901 atggatctgg ccttcgagcc tggaaagctc gagctggttt tccatcgccg ctttcacttt
      961 ttcagcgcgt tcccagagac tgtcagtgcc gagtatatcc tgctgttcga cgaatggtat
     1021 acgcaggctc tcggcaacgg catgcatcag cctttcggga tcattcttcg cgcgcagttc
     1081 acgccaggcc tctgccgaca ggaacgtctg ttgctcgttc agcctttcga aggcatcgac
     1141 cagtttagtc agggatttct ggtacaggtg ctcttctttt tcgccagcgg gagcgaccga
     1201 atcagcgccc acggcaattg ccaccgggca cttttcgtca gaactgtcga tatcgaccag
     1261 ccgtacgcgg tgcagaccct caatgagaat cttatacgta ttgtcgggca tcttgagcag
     1321 ctgcagaatt tcaccctgca cccctgtttc gtacaggtcg ctcagtgccg ggattgtgat
     1381 ttctgcattg cgttgcgttg ccaggataat cttgcgatca gagtccatgg cgcgacgagt
     1441 cgcctccatg gatttttcac ggccgacaaa aattgaagtt acgctgccgg ggtaaatgac
     1501 catatcccgc agcggcagga gaggaaaacg tactgcgcct tgaccggcca ttaagccatt
     1561 ttctccggtg gggcgacgta aacttcttcg cccgcttgtt tacctttttt accgcgctct
     1621 ttgtcggttt ctgcacgcgt gatgatacgc gccttatcgc cagactcgat catctcgcgc
     1681 gtgacaataa cttcgtcgac accgatcatg gttggtatct caaacatgag atccatcatg
     1741 gcgtcttcaa caatggcacg cagaccgcgc gcaccggtct cacgttggag cgctttttgc
     1801 gcaattgctt gtatcgcacc gtcttcaaac gtgagtttga taccttcgag ttcaaagatc
     1861 ttcttatact gcttcaccag cgcgttacgc ggttcagtca gaatcttgac catcgaaggt
     1921 tcatcgaggt tatctagaga gcgagtgatc ggaagtcggc cgataaattc gggaataaga
     1981 ccgtacttca tcaggtcgtc aggcgttacc tggcgcaggt attcaccgct ctggtctttg
     2041 cgcttcggcg ctttaaaatc aaagccgagc gtcgactttt caacgcgccg cgaaataatc
     2101 ttgtccagac cctcaaatgc tccgccgcaa atgaacagga tattacgcgt atccatctgg
     2161 ataaaatcct ggtggggatg ctttctgccg cctacaggcg gcacactggc aaccgtgcct
     2221 tcaatgatct ttagcagcgc ctgctgcacg ccctcacccg aaacatcccg ggtgattgaa
     2281 gggttatctg acttacgcgc aattttgtcg atttcgtcga tatagataat accggtctgc
     2341 gccttttcga tgttggcatc tgcattctgg tagagacgca gcagaatatt ttccacgtct
     2401 tcaccgacat aacccgcttc ggtgagcgcg gtagcatcta cgagagcgca cggcacctgc
     2461 agtatgcgcg caagagtctg cgcgagaaag gtttttcccg agcccgttgg cccgatcagc
     2521 agaatgtttg acttatcaat ttcaacggct gactggtcag atgaagcatg cttgcgcatg
     2581 aacttaccgc cattttttgc cgcctggtct ttgtcatact gcaggcgctt gtagtggtta
     2641 taaaccgcaa cggacagcgc ccgcttggca gcatcctgac cgatgacata ttcatcgagt
     2701 atattcttta tctcggcggg tttgggcagc ttttgcaccg tcgccggcgt tgcctgcttt
     2761 tccaggtcgt ctgcaaggat gccattgcac aattgcacgc attcgtcgca gatgtgcacg
     2821 cctggcccgg cgatcagccg gttgaccttt gcctgctctt tgccgcagaa tgagcagtgg
     2881 ttacctgaat cgtatgaacg ccgttctgcc atgtgacctt ctttctatta tcgtttagct
     2941 ggcgatttct ttcgaggcat tttcgccgcg ggcagaaatc acgtagtcga tcagccccca
     3001 cttcaccgcc tcgttgccat ccatgtaaag gtcgcgctca gtttcctgct ctatcttttc
     3061 gagcgtctgg ccagtatgca gctggtagag tccattaagc gtgtcacgga tgcgcagcac
     3121 ctcacgcgcc tggatctcga tatctgctgc ctggcctgaa gcgccgccca gtggctgatg
     3181 caacagaata cgcgaatgag gcagcgccga acgcttaccc ttggcacctg cagcaagaat
     3241 cagtgcggca atattcgacg cctgccctgt gcagacggta cgcacttcgg ggcgaatcag
     3301 ctggatggtg tcatagatcg cgaggccaga agatacgtat ccaccgggtg agttgatgta
     3361 gagatagata tccttttccg gatcttcaga ttcgaggaat agcagctgcg caatgatcac
     3421 attcgcgtaa gcgtcatcta tgggaaaccc gagaaagatg atacggtctt tgagcagccg
     3481 cgagtagatg tcgtagtgtc gctcgccgcg gtttgtctgt tcaattacga ttggtgcgta
     3541 tgccatgttt ttgtctcccg gaatcagaaa tcgatggtgc cgaaggctgt atgtatttcc
     3601 aattcgccaa tatgtcgtcc atcacagtat gcacaatttg cctgctcacg cccactcttt
     3661 gtctgaagta cgccttcaaa ccttcgctca acgagtaccg ctgccgaccc aggtttt
//
LOCUS       DAOQJF010000327         3714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15500_length_3714_cov_5.204154, whole genome shotgun sequence.
ACCESSION   DAOQJF010000327 DAOQJF010000000
VERSION     DAOQJF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15500_length_3714_cov_5.204154"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLF85_14270"
     CDS             complement(<1..190)
                     /locus_tag="PLF85_14270"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNE20885.1"
                     /translation="MSKQDLPETTPAEMGDKTVHDTIQRRKTRTVKVGNMLIGSGHPI
                     AVQSMTKTKTADWKATVEQ"
     gene            complement(301..1815)
                     /locus_tag="PLF85_14275"
     CDS             complement(301..1815)
                     /locus_tag="PLF85_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE20886.1"
                     /translation="MTQDTSDPLYRAFFDHSLDGVLLTAPDGSIFAANPAACKLLGRS
                     AEEISRLGRSGIMEAGPGLDRLLEERKVRGSARGEVTAIHHDGRRIPVEVSSEVFVDP
                     AGNQRSIIVMRDLTEQKKIAGEKERYMKLFRLSTEPMCIADPFGCFLHVNPAFVKLTG
                     YTERELSSHPFVDFVHPDDKQRTIDEMKRQVAQGPSIGFENRYICRDGRVIHLSWFAY
                     FDETDNHTYATARDVTDALRMNRELADNERKYRSLVTAMAEGVVFQDAEGKIIATNPA
                     AQMIEGRGEAEMLGKTSSDAQWGAIKDDGSPFPGDEHPAMVTLRTGQPQENVVMGIKR
                     PSGERRWLTINAQPLFTEGQSKPYAVVTTFHDITERRRMQEELQNSENLFRTLTESTS
                     AGIYIVTDDRFIECNPAMCDITGYPLEELKSMPLREIFYPDDRSIADTRVAAFARGEQ
                     VPAQYEIRILRKDNQVRWVNITSATTRYLGTRYDPQVVEVAVRLIREKGYTLPQ"
     gene            complement(1964..>3714)
                     /locus_tag="PLF85_14280"
     CDS             complement(1964..>3714)
                     /locus_tag="PLF85_14280"
                     /inference="COORDINATES: protein motif:HMM:NF015963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HNE20887.1"
                     /translation="LYGAILLANAFFMLFSRNSADLSVLRGFAWGSLVMDAISAAFAA
                     LAVMHGTINGFGWLLFTVFTLFTIGFFLAIRTLDAMRFFFSPGSEAEIIELVKRAKSE
                     GKKIRVRGSAHSVDKAIYTTKLVPFNTNAKFFNLYLDQLDAITNINYQKKQVEVQAGC
                     HLGLDPFDPAQKSTWQNSLLCQLANLGWALPDLGGITHQTVGGFLMTGSSGGSVKYSV
                     ADAIVAFRFIDGNGVVHSADRRKNKDLFDALGVSMGLLGIVTSVTFQCVDNYNIMGQE
                     SCTTTGDCPIDLFGPGNAQKPSLAKFLKETEYTRIMWWPQKGVDKAVVWQARRMQKQD
                     YNKETNPPNGPAKDAFKPVPYEELGDHPVPAELAAGIFYWIVGNLNRLGFIGKIISKY
                     LQPILKFVLNLFNPTDTDKKPPGPQKFWDFYWRSLPMDNQIRDDLLPTEFTELWIPIE
                     KTAAAMKKLKDWYDSQGLSATGTYCCEIYAAKKNNFWLSPAYGKDVLRIDIFWYARNS
                     GNPVRDYYPQFWNLLAEFDFRPHWGKYLPPPDSQQGVDYLKKLYPRWVDFMRLRQKHD
                     PHQVFLSGYWKNHLGL"
BASE COUNT          805 a          970 c         1033 g          906 t
ORIGIN      
        1 tctgttcaac cgtggccttc cagtcggcgg tcttggtctt ggtcatcgac tgcaccgcga
       61 tcggatggcc cgaaccaata agcatgttgc ctactttgac agtgcgggtt ttgcggcgct
      121 gtatggtatc gtggacggtt ttatcgccca tttcagccgg ggtggtttca ggcagatcct
      181 gcttgctcat ggagaatccc tgacctgccg ggcgaacagg ctgcaaatcg aaatcaaaaa
      241 taccgctgcg acaggcaaga ccaatctcac cttccggtga atctgccagg attgcggcaa
      301 ttattgcggc agcgtgtagc ccttttcccg gataagtcgg actgctactt cgaccacctg
      361 cgggtcgtag cgtgtgccca ggtatcgggt cgtggcgctt gtgatattga cccagcgaac
      421 ctggttatct ttgcgcagaa tacggatttc gtattgcgca gggacttgct caccccttgc
      481 gaaagctgct actcgggtgt cggcaatgct gcgatcgtca ggataaaata tttccctcag
      541 tggcatagat ttgagctctt cgagcggata tcctgtaatg tcgcacatcg ccggattgca
      601 ttcgatgaat ctgtcatctg taacgatata gattcccgcc gaagtcgatt cagtcagggt
      661 ccggaaaagg ttttcgctat tctgcagttc ttcctgcatt cgcctgcgtt cagtaatatc
      721 atggaaggtt gtcaccactg cgtatggctt gctttgtcct tcggtaaaca gtggctgtgc
      781 attgattgtt agccagcggc gctcgcccga tggccgctta atacccataa caacattctc
      841 ctgcggttga cccgtccgca gggtgaccat ggcgggatgt tcgtcgccgg ggaaaggtga
      901 accgtcgtct ttgatagctc cccattgcgc gtcacttgaa gttttaccga gcatctccgc
      961 ttcgccgcgt ccctcaatca tctgcgcggc gggattggtg gctatgattt tgccttcggc
     1021 atcctgaaac accacacctt cggccatcgc cgtcacgagc gagcgatact tgcgttcgtt
     1081 gtcagccagt tcgcggttca tgcgcagggc atcggtgaca tcgcgtgccg tggcgtaggt
     1141 gtggttatca gtttcgtcaa aataagcaaa ccaggaaagg tgaataactc tgccgtcccg
     1201 gcatatatag cggttctcaa agccgatgga tggtccctgg gctacctgcc gtttcatttc
     1261 gtcgatggtt cgctgtttgt catcggggtg cacgaaatcg acaaatggat gggaggatag
     1321 ctcgcgctcg gtataaccag taagcttcac aaaagccggg ttcacgtgca gaaaacagcc
     1381 aaaggggtcg gcgatgcaca ttggctctgt cgacaggcga aagagtttca tgtagcgctc
     1441 tttctcgcct gcaattttct tttgctcagt gagatcgcgc atcacgatga tagacctttg
     1501 gtttccggcc gggtcgacga aaacttctga gctcacctca acgggtatgc gccgcccgtc
     1561 atggtgtatg gccgtgacct cgccgcgcgc gctgccgcgc accttgcgtt cctccagcag
     1621 tctgtctagc ccgggtccgg cttccatgat gcccgagcgg cctaatcggc ttatttcctc
     1681 tgcgctgcga cccagcagtt tgcaggcagc ggggttggcg gcaaaaatag agccgtcagg
     1741 ggcggtgagc aaaacaccat cgagactgtg gtcgaagaag gcgcggtaga gtgggtctga
     1801 agtatcctga gtcatataaa catcgtaatt gcttaaccgg gctttgctga ttgtggatag
     1861 acggattctt ctggcgcgaa aggcgccagc cgactatgac ctgcaattgc actggcgcgg
     1921 acaggagttt ttggtcctaa aaatctgcag ccagcgggag ctctcagaga cccaggtggt
     1981 tcttccagta accagagaga aatacctgat gcggatcgtg cttttgccgc aaacgcataa
     2041 aatccaccca gcgcggatat agctttttca gataatcaac tccctgctgc gagtcgggtg
     2101 gtggcaggta tttaccccag tgcgggcgaa agtcaaattc tgccagcaag ttccagaatt
     2161 gcggatagta atcgcgtaca ggatttccgg agttgcgcgc ataccagaag atatcgatac
     2221 gcaggacgtc tttgccataa gccggactca gccagaagtt gttttttttc gctgcataga
     2281 tttcgcagca atatgtaccc gtggcagaga gtccctgcga atcataccag tctttcagtt
     2341 ttttcattgc cgcagcggtc ttttcgatcg gtatccagag ttctgtgaac tccgtcggca
     2401 agaggtcatc cctgatctgg ttatccatcg gcaggctacg ccagtagaag tcccagaact
     2461 tttgcggccc cgggggcttt ttgtccgtat cggttgggtt aaaaaggttc agcacgaact
     2521 tcagaatcgg ctgcaggtat ttgctgatga tcttaccgat gaatccaagc cggttcaggt
     2581 tgccgacaat ccagtaaaaa atacccgcgg cgagttctgc aggtacggga tggtcgccga
     2641 gctcttcgta cggcaccggt ttaaaagcat cttttgccgg cccgttcgga gggtttgttt
     2701 ctttattgta atcctgcttt tgcatacggc gggcctgcca gaccacggct ttgtcgacgc
     2761 ctttctgggg ccaccacatg atacgcgtgt actccgtctc tttcaggaat tttgccagcg
     2821 aaggtttctg cgcgttaccc ggaccaaaaa gatcgattgg gcagtcaccg gtggtggtgc
     2881 acgattcctg gcccatgatg ttgtagttgt ctacgcattg aaaggtgacc gaagtcacaa
     2941 tgccgagcag gcccatcgaa acaccgagcg cgtcgaacag gtctttgttc tttctgcggt
     3001 cagcggagtg aacaacgccg ttaccgtcga taaaccgaaa ggcgacgata gcatcggcga
     3061 cagaatattt caccgaacca cctgaagacc ccgtcatcaa aaaaccaccg acagtctggt
     3121 gtgtaatgcc gccgaggtcg ggcaacgccc agccgagatt cgcgagctgg cagagcaggc
     3181 tgttctgcca tgtagatttc tgcgccgggt caaacgggtc gagaccgaga tggcagccgg
     3241 cctgaacttc gacctgtttc ttttggtaat tgatattggt gatagcgtct agctggtcga
     3301 ggtagaggtt gaagaacttt gcgtttgtgt tgaaaggtac gagctttgtc gtataaattg
     3361 ccttgtcgac cgaatgcgcg gaacccctta cccggatttt cttgccctca cttttggcgc
     3421 gtttgacaag ttcgataatt tctgcctctg agccgggcga aaagaaaaag cgcatggcgt
     3481 cgagcgtacg gatggcgaga aagaagccga tcgtgaacag ggtaaatacc gtgaacaata
     3541 accagccgaa accgtttatc gtgccgtgca ttaccgccag cgcggcgaag gcagcagaaa
     3601 tggcgtccat caccagcgaa ccccaggcaa agccgcgcag tacactcagg tcagccgagt
     3661 tgcgcgagaa cagcatgaag aatgcattgg cgagcagaat ggcgccatac aaac
//
LOCUS       DAOQJF010000328         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15531_length_3710_cov_4.834200, whole genome shotgun sequence.
ACCESSION   DAOQJF010000328 DAOQJF010000000
VERSION     DAOQJF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15531_length_3710_cov_4.834200"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="PLF85_14285"
     CDS             complement(<1..1522)
                     /locus_tag="PLF85_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNE20888.1"
                     /translation="MALSWNEIKDRAVKFSKEWADTTSEDAEAKSFLDAFFDVFGISR
                     KRVGTFEHGVRIPRGLPRPTGLAFASQGGREADHKGIESRTGYIDLLWKGVILIEMKS
                     AGKDLDKAFEQAKEYIQGLKESEVPKYILVCDFKIFRLYDTEERTTVEFKLADFVKNV
                     QHFSLVAGYQKRTYKEQDPVNIKAAELLGKLHDRLEEVGYTGHPLEVFLVRLLFCLFA
                     EDTTIFEKLQFQTFLEQKTAEDGSDLASRLQELFQVLDTPEEKRFKNLDEDLAKFPYV
                     NGKLFEETLPIASFDKKMRQALLDCCYLDWSKISPAIFGSMFQSVMNPEERRNLGAHY
                     TSEKNILKLIKPLFLDELWAEFESVSPPRSSTTPPRTRGGAGGGDINPAQRAKLIALH
                     KKISELKFLDPACGCGNFLVIAYRELRLLEIEILKKLHKSGQGVLDVGDILWLDVDMM
                     YGIEYEEFPARIAEVAMWLIDHQMNMAASEEFGQYFVRLPLKKAANIVHGNALRIDW"
     gene            complement(1626..2555)
                     /locus_tag="PLF85_14290"
     CDS             complement(1626..2555)
                     /locus_tag="PLF85_14290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE20889.1"
                     /translation="MKSDYISVPVSYGKINMQVAGPESDTAILFIHGWPDSHDTWSHQ
                     MEYFSQKYRVGAMDLPGVGGSDAPADRSGYHIDNILPVVSAAVKALKAPDARTPSVAA
                     SGQDHRGPRKVHLVAHDWGAIISWVFASRPEYASQIASYTAVSGPHPALARENLFDKF
                     RSLNPGKMLEGVQQTAKSWYIYLFQLPHVPDFIFNNFGRLVWPRALMQGGVPEGDPLL
                     DASQEEISRNVVNPLNLYREMLQGEWIEVPSRIDIPVQVIIPNHDLAITPEIYSNVTD
                     VAPQAQLNYVDANHWVQRSHPQLVNGLLEKFIS"
     gene            2728..>3710
                     /locus_tag="PLF85_14295"
     CDS             2728..>3710
                     /locus_tag="PLF85_14295"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HNE20890.1"
                     /translation="MLAEPMLLKGVIFCTVLTLSAGCGKTATRELLDADYLGEPKVQD
                     VRVVDLSTDSATLTWQAFPPGSSMVELSTTATFQTIAFASPEINGLRYTFSGLTAKTT
                     YFYRVRNSDRKGRSGAYVPNPVAAFTVPVNLDPSLPRLSCNEIRTLFPSYTSGEYLLD
                     TDGSGPNAPFTAYCDMTFSDGTTAGRWTLVLAYNDNQGPLLSTDTPPAKVTRGINKKE
                     FMPMSRLEPLVRNSTQLAIRTHNTLPNNYIIAKLNTAPLQNLVYNSTGSSLNLFPGFT
                     NAASIDATWMRGSGIGTAQLAASGCAPLDYPYPESIYHPCGNGSGFHFIPR"
BASE COUNT          812 a         1054 c          975 g          869 t
ORIGIN      
        1 cccaatcgat acgcagcgca ttcccatgca caatattcgc cgccttcttc agcggcaacc
       61 gcacaaaata ctgcccaaac tcttcgctcg ccgccatgtt catctggtgg tcaatcagcc
      121 acatcgccac ctcggcgatg cgcgcgggaa actcttcgta ttcgataccg tacatcatgt
      181 cgacgtcgag ccagagaatg tcgccgacgt cgagcacgcc ctggcctgac ttgtgcagtt
      241 ttttgagaat ttcgatctcc agaaggcgca gttcgcggta ggcgatcacg agaaagttac
      301 cgcagccgca ggcggggtcg agaaatttca gctcgctaat tttcttgtgt agggctatca
      361 gcttcgcgcg ttgcgcgggg ttgatgtccc cacccccggc ccctccccgc gtgcggggag
      421 gggtggtcga agaccggggt ggggacacac tctcgaactc cgcccacaat tcatccaaaa
      481 ataacggctt gatcagcttc agaatattct tttcgctagt atagtgcgcc ccgaggttac
      541 ggcgctcttc ggggttcatc acgctctgga acatcgagcc aaatatcgcc ggtgaaatct
      601 tgctccagtc gaggtaacag caatcgagca gcgcctgccg catctttttg tcgaagctgg
      661 caatgggcag cgtctcttcg aagagtttgc cgtttacata gggaaacttc gccaggtctt
      721 cgtcaagatt cttgaagcgt ttctcttcag gcgtatcaag tacctgaaaa agctcctgca
      781 gcctgctcgc gaggtcactg ccgtcttctg cggttttttg ctccaaaaat gtctgaaatt
      841 gcagtttttc aaagatcgtg gtatcttcgg caaagaggca gaatagcaac cgcaccagga
      901 aaacttcgag tgggtggccg gtatagccga cctcttcgag ccggtcgtgc agcttaccca
      961 aaagctccgc cgccttgatg ttgaccgggt cttgttcttt gtaagtgcgt ttctggtaac
     1021 ccgcaacaag gctaaagtgc tgcacattct tcacaaagtc ggcaagtttg aactcgaccg
     1081 tcgtgcgctc ttcggtgtca tagagtcgaa aaatcttaaa gtcacaaacg aggatgtact
     1141 tcggaacctc agactctttc aggccttgaa tatattcttt agcctgctca aaggccttgt
     1201 cgaggtcttt gccggcgctc ttcatttcga ttagaataac gcccttccag agcaggtcaa
     1261 tgtagccggt ccgactctcg attcccttgt ggtctgcctc ccgccctccc tggctcgcga
     1321 aggcgagacc tgtcggtcgc ggcagacctc ggggtatccg taccccatgt tcaaaagtac
     1381 caacacgttt gcggctaatg ccgaacacgt cgaaaaaggc gtcgagaaac gactttgcct
     1441 cggcgtcttc gctggtagtg tcagcccatt ctttcgaaaa tttgaccgcc cggtctttaa
     1501 tctcgttcca gctaagtgcc atggtaggtg gcagatttag aaacagccat atagcccgtc
     1561 gtcaaaaata cggacatttt acctactccc cctcccctac taaggggagg ggccgggggg
     1621 tggggttaag aaataaactt ctctaacaac ccattcacca actgcgggtg tgaacgctgc
     1681 acccagtggt tggcatcgac atagtttagc tgcgcctgtg gggcaacatc tgtgacgttg
     1741 gagtaaatct ctggtgtgat agcaaggtcg tggttgggaa taatcacttg caccgggata
     1801 tcaatccggc tgggaacctc aatccattca ccctggagca tctcgcggta gaggttaagc
     1861 gggttcacaa cgttgcgcga gatctcttcc tggctggcat cgagtagcgg atcaccttcg
     1921 ggtacaccgc cttgcatcag ggcccgcggc cacacgaggc gaccgaagtt gttgaagata
     1981 aagtcaggca cgtggggtag ctggaaaaga tagatatacc atgacttcgc ggtctgctgc
     2041 acaccttcga gcatcttgcc cgggttgagc gaacggaatt tgtcgaacag gttttcgcgc
     2101 gcgagcgccg ggtgcggccc gctgactgca gtatacgaag cgatctgcga ggcatactcg
     2161 ggccgcgagg caaacaccca gctgatgatc gctccccagt cgtgcgcgac gaggtggacc
     2221 tttcggggac cacgatggtc ctggcccgac gccgccacgg agggcgtcct tgcgtcgggt
     2281 gctttaagtg ccttcaccgc cgccgaaacg accggcagaa tgttgtcaat gtggtagccg
     2341 ctgcggtcgg ccggcgcatc gctgccaccg acaccgggca ggtccatcgc accgacgcgg
     2401 tacttctgcg aaaaatattc catctggtgc gaccacgtgt cgtgcgaatc gggccagcca
     2461 tgtataaaga ggattgcggt atcagactcg gggcctgcaa cctgcatgtt gattttgccg
     2521 tacgatacgg ggacggagat atagtcggat ttcatagtgc ctcgcttggg cccgtttagc
     2581 ccatgaatta attcatgggc taaacgggat tccgtgggtc aagaaaatga cggatcgttc
     2641 attttcttgc gtcaattgtt caggattctg tgccactagc tggtcatttt cgattgacgt
     2701 tgccagcatt tttaacgtgc gcaatccatg cttgctgagc ccatgttgtt gaaaggagtt
     2761 atcttctgca cagtgctcac cttaagtgct ggctgcggaa agacagccac tcgcgagcta
     2821 ctcgatgcag attacctcgg tgaacccaag gtccaggatg tgcgcgtggt agacctttcg
     2881 accgattcag ccacgctcac ctggcaggcc tttccacccg gcagcagcat ggtcgaactg
     2941 agcacaactg ctacttttca aacgattgcc ttcgcttcac ctgagatcaa cggtctcagg
     3001 tatacctttt ccgggctgac cgcgaagaca acatacttct accgggtgcg taattctgac
     3061 cgcaagggac gttctggtgc ttacgtgccg aacccggtgg ccgcattcac tgtaccggtc
     3121 aatctggacc catcgttgcc gcggctcagc tgcaacgaaa tccgcacgct ttttccttcg
     3181 tacacgagcg gcgaatacct gctggataca gacggtagcg gccccaacgc acctttcacg
     3241 gcttactgcg acatgacatt ttctgacggc acaaccgcag gccgctggac cttggtgctc
     3301 gcttacaacg acaaccaggg tccgctactc agcaccgata caccgcctgc caaggtgacg
     3361 cgtggtataa ataagaagga gttcatgccg atgtcacgcc ttgaaccgtt ggtacgaaat
     3421 tcaacccagc tggcgatacg gacgcacaat accctaccca acaattacat cattgcaaag
     3481 ctgaacacag cgccgctgca gaatcttgtt tataacagca caggttccag cctcaatctt
     3541 tttcccgggt tcacaaatgc agcatccatc gatgccacct ggatgcgcgg ttcaggaatc
     3601 ggcactgccc aactcgctgc gagtggttgt gctccccttg actaccctta tcctgaaagt
     3661 atctaccatc cctgtggtaa cggcagtggc ttccacttta taccacggaa
//
LOCUS       DAOQJF010000329         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15557_length_3705_cov_3.880548, whole genome shotgun sequence.
ACCESSION   DAOQJF010000329 DAOQJF010000000
VERSION     DAOQJF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15557_length_3705_cov_3.880548"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..255)
                     /locus_tag="PLF85_14300"
     CDS             complement(43..255)
                     /locus_tag="PLF85_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20891.1"
                     /translation="MAVVLLSTMVTPVATRPLDTSALQQIYIAEAPRLFGYLVRKTNP
                     DTAQDIVQESFARLYNRMNRSVRHLP"
     gene            415..1128
                     /locus_tag="PLF85_14305"
     CDS             415..1128
                     /locus_tag="PLF85_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNE20892.1"
                     /translation="MSLWQRFKRALRSIFGGWVSEMEDPALILEQNIRDMRDKIPKMN
                     ENIAMLRANAKMVEKEKAKLEGEQAKITANIKAAIGQSREDIAAEYALQLEHNKTQVA
                     KLEQQLEQAKAAAEKGEEAKAAFMKELDRKTKEALDAIETAKRAKWQKEVADTMEQFT
                     AGGVDHTHDEMVKRIEKDSAMSEARLDMAVSKGDKDKAKIEADAEKIRAAELVKQFKI
                     EMGVTDSSGGSQEKTGVKN"
     gene            complement(1152..1898)
                     /locus_tag="PLF85_14310"
     CDS             complement(1152..1898)
                     /locus_tag="PLF85_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE20893.1"
                     /translation="MAKPLLTVCGLSSVQAIASHTPERIERLFFDAERAPFFAKACRL
                     LAEKRKVYRMVSPEELKKITDTAHHQGVAAVITPPPHSELNALRLQQGTLCLHDVLNP
                     HNVGAILRTAAFFGIADIIVSEQSFAAAMTASAWRVAEGGLTYCQLYTYSAARVFFSE
                     AEKKQWFTAAAVRPEKNVLAGLSGVLQKNAGTAVICLGNEENGLPADFTKKCSATFTI
                     PGSGNIESLNVSVAAALCLEKLAGSTGIIR"
     gene            1998..3047
                     /gene="pyrC"
                     /locus_tag="PLF85_14315"
     CDS             1998..3047
                     /gene="pyrC"
                     /locus_tag="PLF85_14315"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNE20894.1"
                     /translation="MPAPQGTTIEIRRGDDWHLHLRDGADMASVLPHTAAQFARAIVM
                     PNLKPPVTTVAEASAYRDRILTALPKGMAFEPLMTLYLTNGTTAKDIAEAKQSGFVHA
                     VKLYPAGATTNSEAGVGVVKNLYPVVEAMEKADLPLLVHGEVTDPAVDVFDRERVFME
                     QEMLPLVQKFPNLRVVFEHITTADAADFVEAQSARVAATITAHHLLLNRNALFTGGVR
                     PHHYCLPVLKREEHRQRLVRAAISGNAKFFAGTDSAPHAKHTKENACGCAGIYTAFGA
                     VELYAEVFDRADSLDKLEFFLSEAGARFYGLQLNAGHMRLKKEKSPVPDSFAMGSGEL
                     VPLRAGGELEWKIIP"
     gene            3029..>3705
                     /locus_tag="PLF85_14320"
     CDS             3029..>3705
                     /locus_tag="PLF85_14320"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNE20895.1"
                     /translation="MEDNTMKIFRRLLLAFCIFTAVGSAHAVTRTDDVSGQYWLGYIT
                     SYRFSEKNSLWNDWHLVPGSFFVSRHGLTRHLSQQVSVTGGYAWGFLTTPGSGSERLD
                     RFEHRPWAQLLMNIPLGTKYTFSNRIRYESRFRQNVTNGETDTGFVFNHRVRFMLAFR
                     RPLTGLRLWDETPFVVVGNEFLLNFAPQITGNQLDQTRTWLMLGYQTGKITLQGGYMY
                     RYVPSTTP"
BASE COUNT          874 a         1050 c         1058 g          723 t
ORIGIN      
        1 ttccctcggt gagattgacc tcgctaccct gtgcagtgat ctttacggta aatgccgtac
       61 cgagcggttc atgcggttat agagccgggc gaaggactcc tggacgatat cctgcgcagt
      121 atcggggttc gtcttgcgaa cgaggtaacc gaaaaggcgg ggagcctcag caatataaat
      181 ctgctgcagg gcactggtgt cgagaggacg cgtagccacg ggagtcacca tggtcgataa
      241 aagcacaact gccacaacca ttttccgttc tctcaaaccc ggggttttat tttgcaccat
      301 ctgcagggaa gagcgccggg cggcgattac cggacacggt attttgcacc gatggctcac
      361 gaccggcaaa ggcaaaaacg cttgccctac gcaccgaacg tcacaccgta acccatgagt
      421 ttatggcaac gttttaagcg cgcactgcgc tctatcttcg gcggttgggt ttcagagatg
      481 gaagaccccg cgctgattct tgaacagaat atccgcgaca tgcgcgacaa aattcccaag
      541 atgaatgaaa acatcgcgat gctgcgcgca aacgcgaaaa tggtcgaaaa agaaaaagcc
      601 aagctcgaag gtgagcaggc gaagattacc gctaacatca aggccgcgat tggccaaagc
      661 cgcgaagaca tcgccgctga atatgccctg cagctcgagc acaacaagac gcaggtagca
      721 aaactcgaac agcagcttga gcaggcaaag gctgcggcag aaaaaggcga agaagccaag
      781 gcggctttca tgaaagaact cgaccgtaaa accaaagaag cgctcgacgc gattgaaact
      841 gccaagcgtg cgaagtggca aaaagaagtc gccgacacca tggagcagtt caccgcgggc
      901 ggcgtcgacc acacgcacga tgagatggtg aagcgcatcg aaaaagacag tgcaatgtct
      961 gaagcgcgtc tcgatatggc ggtcagcaaa ggggacaaag ataaagcgaa gatcgaggcc
     1021 gacgctgaaa agattcgcgc ggctgaactc gttaaacagt tcaaaatcga gatgggtgtc
     1081 accgactctt cgggcggcag ccaggaaaaa accggcgtta agaactagca ggctcctgaa
     1141 aaaggagcca gctagcgaat aatgccggta gaaccggcaa gtttctcgag gcacagcgct
     1201 gcggccaccg aaacattgag cgactcaatg ttgcccgaac cgggtatggt gaacgtagcg
     1261 ctgcattttt ttgtgaagtc ggccggtagg ccgttttctt cgtttccgag gcagatgaca
     1321 gccgtaccag cgttcttctg cagtaccccc gagaggccgg ccaaaacatt cttttcgggg
     1381 cgcacagcag ccgcggtgaa ccactgcttt ttctctgctt cagaaaaaaa tacccgcgca
     1441 gcgctatacg tgtagagctg gcagtaagtc aggccacctt ccgcaacgcg ccaggctgac
     1501 gcggtcatcg cggcggcaaa actctgctcg ctaacgataa tatcagcaat accgaaaaag
     1561 gcagctgtgc gcagtatagc tcccacgttg tgcgggttca gcacatcgtg caggcagagc
     1621 gtaccctgct gaagccgcag ggcattcagc tcgctgtgcg gtggcggagt gatgacggcg
     1681 gcaacaccct ggtggtgcgc agtatcggta atttttttca gttcttcggg actgaccatg
     1741 cggtagactt tgcgtttctc ggcaagcaga cggcacgcct tagcgaagaa cggcgctcgt
     1801 tccgcatcaa agaacaggcg ctcgattcgt tcgggagtgt ggctggcaat ggcctgcacc
     1861 gaagacagac cgcagaccgt gagcaaaggc ttagccattg gtgcccaatg ctgcaaaccg
     1921 atatgagccg gcattagttt tgcgctgcga gaaactttta gacttgcagg cgtcattcgc
     1981 gcgccgcttg cgtcagcatg cccgcgcccc aaggaaccac gatcgagata cgccgggggg
     2041 acgactggca cctgcacctg cgcgacggtg ccgatatggc gagcgtcctg ccacacacgg
     2101 cggcgcagtt tgcccgcgcg attgtcatgc cgaatctcaa gccgccggtc accaccgtcg
     2161 cagaggcgag tgcgtaccgc gaccgcatac ttaccgccct gcccaaggga atggctttcg
     2221 aaccacttat gacgctctac ctcacgaacg gcaccacggc gaaagatatt gcagaagcga
     2281 aacagtcggg ctttgtccac gcggtgaagc tttaccccgc cggtgcaacg accaacagcg
     2341 aagccggcgt aggcgtcgtg aagaaccttt atcccgtcgt cgaagccatg gagaaggccg
     2401 acctgcctct gctcgtgcac ggcgaagtga ctgatcctgc tgtagatgtc ttcgaccgcg
     2461 aacgggtttt tatggagcag gagatgctgc cgctggtaca gaaattcccc aacctgcgtg
     2521 tagtatttga acacattacg accgccgatg cagccgactt tgtcgaggca caatcggcgc
     2581 gcgtcgcagc gacaatcacg gcgcaccacc tgctgctgaa ccgcaatgca ctctttaccg
     2641 ggggcgtccg gccgcaccac tactgcctgc cggtgctcaa gcgcgaggag caccgccagc
     2701 gcctggtccg cgcggcgatt tcaggcaacg caaagttttt cgccggtaca gacagcgccc
     2761 cccatgcaaa gcatacgaaa gagaatgcct gtggctgcgc cggtatctac accgcctttg
     2821 gcgcagttga actgtatgca gaagtttttg atcgggcaga ctcgctcgac aagctggagt
     2881 tctttctttc tgaagcaggt gcccgtttct acggcctgca gctcaacgct ggacacatgc
     2941 gcctgaagaa agaaaaatcc cctgtgccgg atagctttgc gatgggttcg ggtgaactgg
     3001 tgccactgcg cgccggtggt gaacttgaat ggaagataat accatgaaaa tttttcgcag
     3061 actattactg gcgttctgca ttttcacggc agttggcagc gcccatgcgg tgacgcgcac
     3121 agacgatgtc tcgggccagt actggctggg ttacattacg tcttaccgtt tctcggaaaa
     3181 aaactcgctg tggaacgact ggcacctggt acccggttca ttttttgtct cacgccacgg
     3241 gctcacccgc cacctgagcc agcaggtcag tgtcaccggc ggttatgcct gggggtttct
     3301 cacgacaccg gggtcgggca gcgaacgact cgaccgtttc gagcaccggc cctgggcgca
     3361 gctgctcatg aatattccgc tcggcaccaa gtacacgttc agcaaccgca ttcggtatga
     3421 atcgcggttt cggcagaacg taaccaatgg ggagaccgat accggttttg tctttaacca
     3481 ccgcgtgcgc tttatgctcg ctttccggcg cccgctgaca ggtcttcgcc tctgggatga
     3541 gacaccgttc gtggtggtcg gcaatgagtt cctgctgaac tttgccccgc agattaccgg
     3601 caaccagctc gaccagacac gtacctggtt aatgctcggt taccagacgg gcaaaataac
     3661 cctgcagggc gggtatatgt atcgctatgt gccatccacc acccc
//
LOCUS       DAOQJF010000330         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15637_length_3694_cov_4.635614, whole genome shotgun sequence.
ACCESSION   DAOQJF010000330 DAOQJF010000000
VERSION     DAOQJF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15637_length_3694_cov_4.635614"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1776
                     /locus_tag="PLF85_14325"
     CDS             166..1776
                     /locus_tag="PLF85_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE20896.1"
                     /translation="MRALQKRWALPLTGLALSAATLLLVSILLVGNRRDTETAVGKEL
                     QAIAAGVAPEIPAKSYASLFYQSGKLRPDLRQASRENAFAQIKEILREGTARYNYLNL
                     GKDNLYSFVIDPASGELFWAVMLHETAFTGEKYVPPAVVKTLLASKSPGFSPVYLSTA
                     SQREWISGYAPIMLEGQVIGLVEVAREVTEVLKAARGSLYPAIGVSIGIILISGGAMF
                     LILQFARRLQKSNSELSDTLGRLTASTAVSRHLTESSSDIVFGLDSDMKIVSLNSAAR
                     RQLGIDPTAATGKDLTGTLCTRKDGGADYLFDPELLQQALLNLRETGEKVNLTATLTS
                     RLTGEPKDYRIRVERVSQAQELQFIGRASSLGDMVVAPLLNRTRLAFTLNNSLLMTEE
                     LATFLSGLCRRYVDEQKVTTYRIMLREMLLNAIEHGNLEIDFDTKTHALMTESYFELI
                     NERRRTEPYRNRVVTVDCLIDAQKIAFRITDEGKGFDHRSMIVKLRDERNETAHGRGI
                     VMTLQEFDKVRYNEKGNSVVLVKYLAGS"
     gene            complement(1846..2859)
                     /locus_tag="PLF85_14330"
     CDS             complement(1846..2859)
                     /locus_tag="PLF85_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE20897.1"
                     /translation="MKALQIPAVGAVRFGGKVSLGVSSFLLLVKFFAYYLTNSQAILS
                     DALESIVNVAAATLALFIIHISTKPADRDHPYGHGKAEYFSSVFEGALISFAAIMIFV
                     ESIDAIIRGRQIMRIDLGLVLIIAAGIANALLGFYLVRLGKRNQSAALVASGRHVLSD
                     FYTSVAVIVGLLLVRITGIQWLDPASALVVGCVLAWTGFHLVRESVHALMDAEDVALI
                     TELGKHFTALRRPGIIRIHHTRVIRSGAYHHIDAHVVVPEFWSVATGHTETNAFEAAV
                     MEQYSHAGEIHFHIDPCRRVYCHACDVAQCPLRRETFARVIPFTFEELTSPTEPEELQ
                     HSG"
     gene            complement(2856..>3694)
                     /locus_tag="PLF85_14335"
     CDS             complement(2856..>3694)
                     /locus_tag="PLF85_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20898.1"
                     /translation="NHLRAVEQSNPATMFALPQRVDTFESHELGFMPDALKVLFREIL
                     SWQQDIGGVYAAAVPVVSDWLQRTGATSILDLCSGAGGPGITLVKALRESGVPSATIK
                     LTDLYPATEVYTQLQAQHAEFVSFEPGSVDATNSRRDETFRVRTLLSAFHHFSPEFAQ
                     KILADAAQNSDGICIMDPFHRDLWHLLSVPIGTTLGTQIYPLVRDRSPFALAMCNLTP
                     VIPAMFFWDSIASVLRGYTEDELLALTRIPECDAFEWRAGTWSYLPVTGLKGVYLTGV
                     RK"
BASE COUNT          863 a         1065 c         1043 g          723 t
ORIGIN      
        1 ggggttgccg cgcgagtcga tcacttcacg ggcacgaaca tcttcgatgg tagctgtcat
       61 gggtttggct ttgcttttgg gctgctctgt aaagaagttt ggcttaaggg aaaaagtcgc
      121 tgcacgattc cttgccaccc tgacggcagc cggctttttt gtcccatgcg cgcgctgcag
      181 aaacgctggg cactgcccct cacaggcctc gcgctctcgg ccgcaaccct gctgctggta
      241 agcatactgc tggtcggcaa ccggcgcgat accgaaacgg cggtgggtaa agagctgcag
      301 gccattgccg caggagtagc gcctgaaatt cccgctaagt cctacgccag cctgttttac
      361 cagtcaggga aactccgccc cgacctgcgg caggccagcc gcgagaatgc ctttgcacag
      421 atcaaagaga tactgcgtga agggactgcc cgctacaatt acctgaacct gggcaaagac
      481 aacctctata gcttcgtgat cgacccggct tcaggcgaac tcttctgggc tgtcatgctg
      541 cacgagacag cattcaccgg cgagaaatat gttccaccgg cagtggtaaa gacccttctc
      601 gcctcaaagt cgccggggtt cagccctgtt tacctgagca cagcctcaca gcgcgaatgg
      661 atttcgggct atgcgccgat tatgctcgaa ggccaggtca ttggtctcgt cgaagtcgcg
      721 cgcgaggtga ccgaggtact gaaggcagcg cgcggcagcc tttacccggc gatcggcgtg
      781 agcatcggca ttatcctcat cagcggtgga gcgatgtttt tgattctgca atttgcgcgc
      841 aggctgcaaa agtcgaacag tgaactttcc gacactctcg gccggttgac cgcatcgacg
      901 gcagtctcgc ggcacctgac cgaatccagt tccgacatag tctttggtct cgatagtgac
      961 atgaaaatcg tctccctgaa ttcagcggcg cgccggcagc tcggcataga tcctacagca
     1021 gccacaggca aagacctcac cgggacactt tgcaccagga aggacggcgg agcagactat
     1081 ctcttcgacc ccgaactgct gcagcaggcc ctgttgaacc tgcgcgagac cggcgaaaaa
     1141 gttaacctga cggcaacgct aacttcgcgt ctcacgggcg aaccgaagga ttaccgcatt
     1201 cgcgtcgaac gcgtctcaca agcgcaggag ctccaattca tagggcgtgc gtcgagcctg
     1261 ggtgatatgg tggtcgctcc actgctcaac cgaacccggc ttgcctttac gctcaacaac
     1321 tcgctgctga tgaccgaaga gctggcgacg tttctttcag gcctgtgccg tcgctacgtc
     1381 gatgaacaga aagtcacaac ctaccgcatt atgctgcgtg aaatgctact caatgcaatc
     1441 gagcacggca acctcgaaat cgacttcgat accaaaacgc atgcactcat gacagaaagc
     1501 tatttcgaac tgattaacga gcgccgccgc acagaaccgt accgcaaccg tgtggtcacc
     1561 gtcgactgcc tgatcgacgc gcagaagatc gcctttcgca tcaccgacga gggcaaggga
     1621 tttgaccacc gctccatgat tgttaaactt agagatgaac gcaatgaaac cgcgcacggc
     1681 cgcggtatag tcatgacact gcaagaattc gacaaggtgc gctacaatga gaagggcaac
     1741 tcggtcgtgc tggtgaagta tctagcaggc tcctgagaaa ggagcctgaa gcaggtttcg
     1801 gaaaccgtat aaggctgcag acagcgggag tttttccgga acctgctacc cagagtgctg
     1861 cagttcttca ggctctgtcg gtgacgtcag ctcttcaaac gtaaaaggta tcacccgcgc
     1921 aaaagtctct cgccgcaggg ggcactgcgc cacgtcgcac gcatggcaat acacccggcg
     1981 acaagggtcg atatgaaagt gtatctcacc cgcatggctg tactgctcca taacggccgc
     2041 ttcaaacgca ttcgtctctg tatgcccggt cgccacgctc cagaattcgg gcacgacgac
     2101 gtgcgcatca atgtggtggt aggcgcccga acggatcact cgtgtatggt gtatacgtat
     2161 gatgccaggc cggcgcaagg ccgtgaaatg ttttcccagt tctgtaatga gggcaacgtc
     2221 ttcggcatcc atcaacgcgt gcaccgactc gcgcacgagg tgaaaaccag tccaggcgag
     2281 cacacaaccg acaacaagag ctgaggcggg gtcaagccat tggataccgg taatgcgcac
     2341 gagcaacaga cccacaatca ccgcaacgct ggtatagaag tctgacagta cgtggcggcc
     2401 gctggcgacg agggcagccg actggttgcg cttacccagg cgaacgaggt aaaaacccag
     2461 cagcgcgttc gcaatgccag cggctatgat gagcacaaga ccgagatcaa tgcgcatgat
     2521 ttgccggccc ctgatgatcg catcgatact ttcgacaaag atcatgatcg ccgcaaatga
     2581 aatgagcgca ccttcaaaaa ccgagctgaa atactcagcc ttgccgtggc cgtatggatg
     2641 gtcgcggtcg gccggtttgg tactgatatg aatgatgaaa agcgccagag tcgctgcggc
     2701 gacgttgacg atactttcga gcgcgtcgga aagaattgcc tgcgagttgg tcaggtaata
     2761 ggcaaaaaac ttcacgagca gcagaaaact actgacgccc agcgaaactt tgccgccaaa
     2821 tcgcaccgcg cccaccgccg gtatctgcaa tgccttcatt tgcgcacacc tgtcaggtaa
     2881 acacctttga ggcccgtaac cggcaggtaa ctccatgtgc ccgcgcgcca ttcaaaggca
     2941 tcgcattcgg gtatgcgcgt gagcgccagc agctcgtctt ctgtgtagcc gcgcagcacg
     3001 ctggcgatgc tgtcccagaa aaacattgcg ggtattaccg gcgtcaggtt gcacatcgcc
     3061 aaggcaaagg gggagcgatc acgaaccagc gggtagatct gtgtgcccag cgtcgtgccg
     3121 atcggtacag aaagcaggtg ccacaggtcg cggtgaaaag gatccatgat acagataccg
     3181 tcgctgttct gtgcggcgtc ggccagaatc ttctgcgcga attcgggcga aaagtggtga
     3241 aacgccgaga gcagcgtccg cacgcgaaag gtctcatcgc gccggctgtt ggttgcgtcg
     3301 acgctgcccg gttcaaagct gacaaattcg gcatgctggg cctgcagctg cgtatatact
     3361 tcggtcgcag gataaagatc ggtaagtttg attgtcgcag acggaactcc actttcgcgc
     3421 aatgctttca ccagggtaat acccggccca cccgcacccg agcaaagatc caggatggat
     3481 gtggcgccgg tacgctgcag ccagtcgctc accaccggta cagcagccgc gtagacgccg
     3541 ccgatgtcct gctgccacga gagtatctcg cgaaaaagca ccttgagtgc atcgggcata
     3601 aaaccgagct catggctttc aaaggtatcg acgcgctgcg gcaaggcaaa catggttgcg
     3661 gggttactct gctctaccgc gcgtaaatgg tttt
//
LOCUS       DAOQJF010000331         3677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15765_length_3677_cov_5.584760, whole genome shotgun sequence.
ACCESSION   DAOQJF010000331 DAOQJF010000000
VERSION     DAOQJF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15765_length_3677_cov_5.584760"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLF85_14340"
     CDS             complement(<1..194)
                     /locus_tag="PLF85_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNE20899.1"
                     /translation="MPSTFMGIEIARRGIAVHQQALTTTGHNISNADNKNYARQRVDM
                     ETMHPLYEPSLNRASGAGHLG"
     gene            complement(205..714)
                     /gene="flgN"
                     /locus_tag="PLF85_14345"
     CDS             complement(205..714)
                     /gene="flgN"
                     /locus_tag="PLF85_14345"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HNE20900.1"
                     /translation="MKTQMMSHGQLTGDTHALVSELVEVLRDELAAHHRILQLELTKR
                     EAIIARDGEKLKSSAGEQAKELHRIDLLESRREKLAERILPGQSAINLSLIIQSDAVA
                     APEKKELSRYQMALKSALLELRKLSEVNTRMLSDSRDLFKSMIMSLAGKGADRQHAGT
                     RPVLVDANC"
     gene            1594..2079
                     /locus_tag="PLF85_14350"
     CDS             1594..2079
                     /locus_tag="PLF85_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20901.1"
                     /translation="MILRTLPLLAFFITPALLLAGPTRGRAINDLQRLLAMHQAEMQA
                     VFAGIGSGSPAAPAFFTDVSFLNDRQVESFLLNVSASSQPGQVYSQVSFTRMVAIGGD
                     VRSVSYSLVNDGANVRLVKSSNVNGQLLKAVYDYDVAGRKLKTGAFDGQKTIKNSEYS
                     I"
     gene            complement(2108..>3677)
                     /locus_tag="PLF85_14355"
     CDS             complement(2108..>3677)
                     /locus_tag="PLF85_14355"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE20902.1"
                     /translation="MDAFANIKTDVPYKTIAELWNARASRLADKTYLHFRERAWSYAE
                     MNLQAEKAAGLLKSLEAQRGEHVAVLVPNSPEFLFLWFGAMKGGFTAVTINTLLKAEE
                     LAYIIDDCDARILVATPAFRRLLEPVLPGLSKLEHVVLTEPDTQSSGYKLLPEEMQRA
                     EPYAAAGLTGSDKASMIYTSGTTGHPKGVILSHGNILYNSYVTHQMIDLEESDTALCI
                     MPLFHVNAQIASMMSTLWAGASVVLEDGFKPRSFIGTLKQYRCTTFSGVPTIYNFLNE
                     MKEAEGEDLSFLKACICGAAPMPVEVFNSFEQKFKGKIIEGYGLSEGTCVSSLNPLRG
                     KRKIGSIGLSIPGQEMRIVNANVEGQNIFLPDDTIGEIVIRGPNMMQGYYKKEEANRT
                     AIVDGWLHTGDLGTRDSEGYFYITGRKKEMIIQGGENIYPKEIEEVLYKFDPVLECAI
                     VGIPDAKYGEVVGAFIIPKEGASITAAEVRAYLREKIANYKMPKVIEFLQSFPKTATG
                     KIQKNRIVESYGKV"
BASE COUNT          773 a          964 c          989 g          951 t
ORIGIN      
        1 ccgaggtggc cggcgcccga cgcgcggttg agcgaaggtt cgtacagcgg gtgcatggtt
       61 tccatgtcca cgcgctgcct ggcgtagttc ttgttatctg cgtttgaaat gttatgaccc
      121 gtggtggtga gggcttgctg gtgcacggcg attccgcgtc gtgcaatctc aatccccatg
      181 aaggttgatg gcattgttat ctccttagca gtttgcgtca acgagcaccg ggcgtgtacc
      241 cgcatgctgt cgatctgctc cttttccggc aagactcatg atcatcgatt tgaagagatc
      301 gcggctgtct gaaagcatgc gggtgttgac ctcgctgagc ttccggagct cgagcagcgc
      361 tgacttgagc gccatctggt aacgactgag ctccttcttt tcaggtgctg cgactgcgtc
      421 ggattgaata atgaggctca ggttgattgc agactggcca ggaagaattc gttctgcgag
      481 cttttcgcgc cggctttcga gcagatcgat tcggtgcagc tctttggcct gctcgccggc
      541 agaagatttc agcttttccc cgtcgcgcgc gatgatggcc tcacgcttgg tcagttcaag
      601 ctgcagaatg cggtggtgtg ccgcgagttc gtcgcgcagc acttcaacga gttcactcac
      661 caaagcgtga gtatcgccag tcagctgccc gtgactcatc atctgggttt tcatattttg
      721 aagatcggcg gatcgctaaa aggcatgagc acttattcgg tccggctggt tttgggcacc
      781 ttgtaggcac agcttttcgc ccgggtagga ttcgggccta ttttgggtta tcccggcggt
      841 aaaatgtgaa gtatatttag ggggagttcc gcattcaatg gtcaatttct gcgatcggga
      901 gcgcgttttt agggtaaatc ctgtactcct gtgaatattt tagcgctaaa gaagaattct
      961 cgttagtttc gcattggtag gtaataaaga tttctggctg cgaattgcgg cgcgcctgat
     1021 aacgcctctg ccaataggag tgattcctca cggctcttgc acagcccgga ataggactac
     1081 gtaaatactc ctccataagc cttggcaggt agtgccggat cgcaaaatcc agcatgcggg
     1141 agacggactc gccggaatgg actgcacgct gccacaagat agcgtagagc atttggtcag
     1201 cataccatgc cacccgcacg tacttctcgc cggtcgcgca attgtaccta cgcgcggtgg
     1261 ccgatttccg atccctgccg cgccaatgcc tgaggtaagt ttttgccagg cgccgcagta
     1321 gctccgattc tgaccagcat accccgttac ggcgcagaat tttcagggca gcccgaaatc
     1381 ttcttctcga tgcaacccgc atggtcactg aagttgagtg gtactgcaag ggactaatat
     1441 tctgcattct atatattaaa tacctaaaat gccccgattt ttctcatgct ttgtaatttt
     1501 ttttgacggt atctgcctgc ctccatattt ttttacagcg gtagtgtggg gcacccgcga
     1561 ccgtgttgat tttatagatt tggaggattt tgcatgattc tcagaacttt gccactcctg
     1621 gcattcttta ttactccagc cttgctgctg gcaggcccga caagaggccg ggcaattaat
     1681 gatttgcagc gtctgctggc catgcaccag gccgaaatgc aggcagtctt cgcgggcatt
     1741 ggatcaggaa gcccggcggc accagctttt ttcaccgacg tctcgttcct gaacgatcgc
     1801 caggtagaat cgttcttgct taacgtctcg gcgtccagcc aaccggggca ggtttatagc
     1861 caggtcagct tcacgcgcat ggtagcgatt ggtggagacg tgcgcagcgt ttcgtactct
     1921 ctcgtcaatg acggcgcaaa tgtacggctg gtcaagagca gcaatgtcaa cgggcagctg
     1981 ctgaaagcgg tgtacgacta cgatgtggca ggacgcaagc tgaagaccgg tgcctttgac
     2041 gggcaaaaga caatcaagaa ttcagagtat tctatctaaa gatgtcctga gtgcctcatt
     2101 gcggcactca gacctttccg taactttcga caatccggtt cttttgtatt tttccggttg
     2161 cagtcttagg aaatgactgc aaaaactcga tcaccttggg catcttgtag ttggcgattt
     2221 tttctcttaa gtaggcgcgc acttcagcag cggtaatgct ggccccttct ttgggaatga
     2281 taaatgctcc gacgacttcg ccgtactttg catcaggaat tcccacgatg gcacattcga
     2341 gcacggggtc aaacttgtag agaacctctt cgatttcttt ggggtagata ttttcgccac
     2401 cctggatgat catttctttc ttgcgaccag taatataaaa atatccctcg ctgtcgcgcg
     2461 tacccaggtc gcccgtgtgc agccagccgt cgacaatggc cgtgcgattg gcctcttctt
     2521 tcttgtagta accctgcatc atattcggcc cgcgtatgac aatttcgccg atggtatcat
     2581 caggcaggaa tatattctgt ccttcgacat tggcgttgac gatgcgcatc tcctggcccg
     2641 gtatagagag gccaatggaa ccgatcttgc gcttaccgcg caggggattc agcgatgaca
     2701 cgcaggtgcc ctctgaaaga ccataaccct cgatgatctt gcccttgaat ttttgttcga
     2761 aactgttgaa gacttcaacg ggcatgggag cggcaccgca gatacaggcc ttcagaaaag
     2821 acaggtcttc gccctcggct tccttcatct cgttaaggaa gttgtagatc gtgggcacgc
     2881 cgctaaaggt cgtgcaacgg tactgcttca gtgtgccgat gaacgagcgc ggcttaaaac
     2941 cgtcttcgag caccaccgaa gcgccggccc acagcgtcga catcatagag gctatctgcg
     3001 cattgacatg aaacaaaggc atgatacaaa gtgcagtatc actctcttca agatcgatca
     3061 tctggtgcgt tacataacta ttgtagagga tgttaccgtg cgacaatatc acacctttgg
     3121 ggtggccggt cgttccggag gtatagatca tcgaagcctt gtcgctaccc gttaaccccg
     3181 ctgcggcata cggttctgcc cgctgcattt cttccggcag taatttgtaa ccacttgatt
     3241 gcgtgtccgg ttcggtcagg accacgtgct cgagcttact gagaccagga agcaccggtt
     3301 cgagcagcct acgaaacgca ggagtggcca ccaggatgcg cgcgtcgcag tcatcaataa
     3361 tgtaagcgag ttcttctgcc ttgagaagcg tgttaatcgt gaccgcggtg aatcccccct
     3421 tcatggcgcc aaaccacagg aacaggaact cgggtgaatt gggcaccagt accgcgacgt
     3481 gttcacccct ctgcgcttca agggatttga ggagtcctgc tgccttttcg gcctgcaggt
     3541 tcatctctgc atacgaccac gcgcgctcgc gaaagtgcag gtaagttttg tcggccaacc
     3601 tactcgcgcg cgcgttccag agttcggcta ttgtcttgta cggaacgtcg gtcttgatgt
     3661 tggcaaaggc atccatg
//
LOCUS       DAOQJF010000332         3660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15889_length_3660_cov_4.210818, whole genome shotgun sequence.
ACCESSION   DAOQJF010000332 DAOQJF010000000
VERSION     DAOQJF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15889_length_3660_cov_4.210818"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PLF85_14360"
     CDS             complement(<1..1608)
                     /locus_tag="PLF85_14360"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNE20903.1"
                     /translation="MSESTGAPEGGKFRYRADMAPPQPAPVASKGFKCPNCGGPVNLE
                     LPGKSVTIRCPKCSSILEPEHEVLKLRDKYNEQFPHRQWIPMGTEGTLNGIKFKCVGM
                     VVRGDDDGGEWSEYLLFNPYHGYRYLVESSGHWTLIEQAPGLGFDKSGRPGWYGPTGT
                     IKVAGKKLKYFTHYRATVKNIIGEFPWQATIGETNEVTEYINPPYQASCENVTQYFDK
                     KGRQVDIASLIAAEAKSSSAGSDDEDDEEEDDDEGVDIDAIIAKHGLTKRITESNWSI
                     GEYKYPEEIQAAFKLEEMPAKLGFGMCEPNPMRARYLVSLAITGLLFLATCGTCSVAS
                     GRAEEKVVFDKAITLNTAEFELKKEGTTDYLEFNFEPGTIELTKESNVEFDFRGNLNQ
                     EWISLNVFMINEQTGRGFIYDGELSYYYGGSGEDKWSEGGNSTDFTTEKMPPGQYYLF
                     VAGATNIGVNEFKRNLQVYGERVEAPPAVPATGPLGKTQPGKPGAKAITPAKPAAAAA
                     QATPTPASAAKPGGKLLPLDFFGKPTAP"
     gene            complement(1605..2285)
                     /locus_tag="PLF85_14365"
     CDS             complement(1605..2285)
                     /locus_tag="PLF85_14365"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNE20904.1"
                     /translation="MKQSTCPSCGAPLTFENAHSLYAVCKYCRTMSVQSEDALKEVGQ
                     TAALVEDGSPLQLGATGILDGKTFKIVGRIQYKHPVGYWNEWFLSVDEEAGDAWLGEA
                     NGMYFFTRQKKDAKLPDGLAFSELYAGGSVEIDGKEFFVKDIQTTKVVSGEGELPFSF
                     EGEYEASVVDCVREDGTFATLDFSETPPLVFTGRAVSFRDLRLSRIRSVYGFKSGDAE
                     PVTAGAQA"
     gene            complement(2357..>3660)
                     /locus_tag="PLF85_14370"
     CDS             complement(2357..>3660)
                     /locus_tag="PLF85_14370"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNE20905.1"
                     /translation="PDAKRAEIEADIKATYEKRPPLAMVDSEKGITNLHAGNDVIIDA
                     SMPVVIRDSGQMWGPDGKLQQCKAVIPDTSYAEFYQVGFDFCRENGAFDPRTMGNVAN
                     VGLMAQQAEEYGSHDKTFKAPADGVIRIVDTAGKVLTEHQVEQGDIWRACQAKDEAIR
                     DWVGLAVRRARATGSPAVFWLNKDRAHDAQVIAKVNTYLKDHDTSGLEVLVLSLPEAM
                     KYTLERVKAGKDTISVTGNVMRDYLTDLFPILELNTSAKMLSIVPLLNGGGLFETGAG
                     GSAPKHVQQFQKEGYLRWDSTGEFLALGVSLEHLAETFKNPKAKVLADTLDVAFGKFL
                     DNNKSPARKVGEIDNRGSHFYFALYWAQALAAQTADKDLQAKFAPVAKQMAENEAKID
                     KELIDAQGKPVDMGGYYHPDDAKTAKAMRPSATLNSIIDSI"
BASE COUNT          672 a         1068 c         1038 g          882 t
ORIGIN      
        1 aggcgcggtc ggtttaccaa aaaagtcgag cggcaagagc tttccgccgg gcttggcggc
       61 agacgcaggt gtcggtgttg cctgtgccgc tgctgccgca ggtttcgctg gcgtgattgc
      121 cttggctccg ggcttgcccg gctgtgtctt gccgagcggg cccgtagcag gcacggcagg
      181 cggagcttcc acgcgttcgc cgtagacctg caggttgcgc ttgaactcat tcacgccgat
      241 attggtcgcg ccggcgacga acaggtagta ctgtcccggt ggcatcttct cggtcgtgaa
      301 atcggtcgaa ttgccgccct cggaccattt atcctcgccg gagccgccat agtagtacga
      361 gagctcccca tcgtagatga aaccccgccc cgtctgttcg ttgatcatga acacgttgag
      421 gctaatccat tcctggttca ggttgccgcg gaaatcaaac tcgacgtttg actctttggt
      481 cagttcaatg gttccgggtt cgaagttgaa ctcgaggtaa tcggttgtac cttctttctt
      541 cagttcaaat tcagcagtgt tgagcgtgat agccttgtca aagacgactt tctcttctgc
      601 acgtcccgag gcgacggagc aggtaccgca ggttgccagg aacaaaaggc ccgtgatcgc
      661 cagcgacacg aggtagcgtg cgcgcatcgg gttgggttcg cacatgccaa aacccaactt
      721 cgctggcatc tcttcgagct tgaaggctgc ctgtatttct tcaggatact tatactcgcc
      781 gatcgaccag ttactttcgg tgatgcgctt ggtgaggccg tgttttgcga tgatggcatc
      841 gatatcgact ccctcatcgt cgtcttcttc ttcgtcgtcc tcgtcatcgc tgccggctga
      901 actgcttttt gcctcggcgg cgatcagaga agctatatcg acttgccggc cttttttgtc
      961 gaagtactgc gtgacatttt cacacgaggc ctggtagggc ggattgatgt attcggttac
     1021 ttcattggtt tcgccaatgg ttgcctgcca gggaaactcg ccaatgatat tctttactgt
     1081 ggcgcggtag tgcgtgaagt atttgagttt tttgcccgca accttgatcg ttcccgtcgg
     1141 gccataccaa cccgggcgtc cgctcttatc aaagcccagc cccggcgcct gttcgatcag
     1201 cgtccaatgg cccgaactct cgaccaggta gcggtagccg tggtacgggt taaagagcag
     1261 gtattcgctc cactcaccac cgtcgtcgtc gccgcgcacg accatgccca cgcacttgaa
     1321 cttgatgcca ttcagcgtgc cttcggtacc catcggtatc cactggcggt gcgggaactg
     1381 ctcgttgtat ttatcgcgga gcttgagaac ttcgtgttct ggctcgagaa tagaagaaca
     1441 tttcgggcag cggattgtga ccgacttgcc gggcagttcg aggttgaccg ggccaccgca
     1501 gttcgggcac ttaaacccct tcgaagcaac cggcgccggc tgcggcggcg ccatgtccgc
     1561 gcggtaacgg aacttgccgc cttctggcgc gcccgtgctt tcgctcatgc ctgcgccccc
     1621 gctgtaaccg gttctgcgtc gcccgattta aagccataca cactgcgtat gcgcgagagg
     1681 cgcaggtcgc gaaaggagac tgcgcggccg gtaaaaacca gcggcggcgt ctcggaaaaa
     1741 tcgagagtcg cgaaggtgcc gtcttcacgc acacagtcga ctaccgacgc ctcgtattca
     1801 ccctcaaaag aaaaggggag ctccccctcg cctgaaacaa ctttcgtcgt ctggatatct
     1861 ttcacaaaaa attctttgcc gtcaatttcg actgacccgc cggcataaag ctctgagaat
     1921 gccagcccgt cgggcaattt ggcgtccttt ttctgccggg tgaagaagta catgccattc
     1981 gcctcgccca gccacgcgtc gcctgcctct tcgtcgaccg aaaggaacca ctcgttccag
     2041 tagcccacgg gatgtttgta ttgtatacgg ccgacgatct tgaaagtttt accgtcgagt
     2101 atgcctgttg cgccgagctg cagtggcgaa ccgtcttcaa cgagcgccgc ggtctggccg
     2161 acctctttca gtgcatcttc tgactgcacc gacatggtcc tgcagtactt gcagactgcg
     2221 tagaggctgt gcgcgttctc aaaagtgagc ggcgcaccgc acgacgggca ggtcgattgc
     2281 ttcacggcgg tcagcctatg aaaattggtc tattgtgtca cctgaaaaat atggggcact
     2341 tcgtgcccca tatttttcag atactgtcta tgatcgaatt cagcgttgcg cttgggcgca
     2401 tcgccttcgc agtctttgca tcgtccgggt gatagtaacc acccatgtcg accggcttgc
     2461 cctgcgcgtc gatcagctct ttgtcgatct tcgcttcgtt ctcagccatc tgcttcgcta
     2521 ccggcgcaaa cttcgcctgc aggtctttgt cagcggtctg cgccgcgagc gcctgtgccc
     2581 agtagagagc gaaatagaaa tggctgccgc ggttgtcgat ttcaccgacc ttgcgtgcag
     2641 gcgatttgtt gttatcgaga aacttgccga aggcaacgtc gagcgtgtcg gcgagcacct
     2701 tggcttttgg gttcttgaaa gtttctgcga ggtgctcgag cgaaacgccg agcgccagga
     2761 attcaccggt tgaatcccag cggaggtaac cttctttctg gaactgctgc acgtgtttcg
     2821 gggcagatcc gccggcgcca gtttcgaaca ggccaccgcc attcaagagc ggcacaatcg
     2881 agagcatctt cgcgctggta ttcagttcga gaatcgggaa caggtcagtc aggtagtcgc
     2941 gcatcacgtt gccggtgaca gagatcgtgt ctttaccggc ctttacgcgc tcgagtgtgt
     3001 acttcatcgc ttcgggcaga gacagcacca gcacttcaag gcccgaggtg tcgtggtctt
     3061 tcaggtacgt attcaccttc gcgatcacct gcgcatcgtg cgcgcggtct ttgttgagcc
     3121 agaaaactgc cgggctgccg gtcgcacgcg cacggcgcac cgcgagacct acccaatcgc
     3181 gtatcgcttc gtcttttgcc tggcaggcgc gccagatgtc gccctgctcg acctggtgtt
     3241 cagtgagcac cttgcctgca gtgtcgacaa tgcgtatcac tccgtcagcg ggagctttga
     3301 aggtcttgtc gtgcgagccg tattcttcgg cctgctgcgc catgagaccg acgttcgcaa
     3361 cgttgcccat cgtgcgcggg tcgaatgcgc cgttttcgcg gcagaaatca aaaccgacct
     3421 ggtagaattc ggcataagac gtatcgggaa tcacggcctt gcactgctgc agcttgccat
     3481 cggggcccca catctggccc gaatcgcgga tcaccaccgg catcgaagcg tcgatgatca
     3541 cgtcgttgcc ggcgtgcagg ttcgtaatac ctttttcgga atcgaccatc gcgagcggcg
     3601 ggcgtttttc gtatgtcgcc ttgatgtctg cttcaatttc ggcgcgtttt gcgtcaggca
//
LOCUS       DAOQJF010000333         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_15914_length_3657_cov_6.191005, whole genome shotgun sequence.
ACCESSION   DAOQJF010000333 DAOQJF010000000
VERSION     DAOQJF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15914_length_3657_cov_6.191005"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLF85_14375"
     CDS             <1..702
                     /locus_tag="PLF85_14375"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNE20906.1"
                     /translation="APAADRKKDQSYYLYGMSQESLARTIFPLAKMIKDEARRIAEAN
                     NIPVARKPDSQEICFIPDNDHKAFLKREGVSMRPGYFRDRLGAILGKHEGSVGLTIGQ
                     RKGLGVAGFAEAQFVLEILSNGDVILGPREELERRTFYIRDVVFQGVAEDDRAWSEGV
                     PCNVQIRYNGQPLAAVAYKVETPLMASLRIELDTPAWAVTPGQAGVLYDRDGGFIIAG
                     GKIRLNQGATVHAAD"
     gene            1181..1852
                     /locus_tag="PLF85_14380"
     CDS             1181..1852
                     /locus_tag="PLF85_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20907.1"
                     /translation="MPAYTSLKLKSGLPWLAAFLLMTAVALFLRTPETVGRYLLLLTI
                     AWVVLFFGWARTTHSASLRQILLFSTILSVFQVFPDWVLSRHLGVLVFPPDGLFKWDT
                     VSGYMALLWTIPASQILIAYSVAHERWGYKLAYAAAILVGAILFIGSEELLWMLPSWY
                     AINVIKWGHVARYIILPEILLSFMIVFVFGRVKEKSLLWYTCGAALVSFIYTAAAVLS
                     YHLMG"
     gene            1857..2951
                     /locus_tag="PLF85_14385"
     CDS             1857..2951
                     /locus_tag="PLF85_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNE20908.1"
                     /translation="MQPLKDQFFSKTYITTLAADVKTAAPDFDAAAFTRQVLDRKWKN
                     RELKDRMHHVADCLHAALNRDYKSSIRLLTPIVQQRIKEGTNSFGDMIFPDFVERFGL
                     EDFKTSVRALEVFTAACSSEFAVRPFIVRYPQEMMRQMLEWSKHKSEHVRRLASEGCR
                     PRLPWAMALPEFKKDPRPVLKVLENLKSDTSEYVRRSVANNLNDISKDNLEVALQTAE
                     RWFGKNKATDALVRHALRNELKKGNKRALALFALPHRTKARINKLEITPARIRIGESV
                     HIHFTLESTATERLRLEYWVDFVKAKGHATRKVFQIFEGDFSAGATRAFRKKHRFQDF
                     TTRKHYAGAHRIAIAVNGSAKAETEVHLAD"
     gene            complement(2948..3448)
                     /locus_tag="PLF85_14390"
     CDS             complement(2948..3448)
                     /locus_tag="PLF85_14390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE20909.1"
                     /translation="MSTKAIGIGLAAILGLLLFAGGLLYNKYRIAPVLAITDLEALDA
                     KGAPTRVWSQNGRGAVVVFYASWCHDCAREMPKLAQALRGYLRGVDAIALTDEDITAM
                     VRYQYRTAHPFRFYALPADFGAYGIHAIPTTYIVNANGETIYAKVGDVDWTSPEIASA
                     VKQAGL"
BASE COUNT          841 a          983 c          997 g          836 t
ORIGIN      
        1 gcacccgccg ccgatcgcaa gaaagaccag agctactacc tctatggcat gtcgcaggaa
       61 tcactcgcgc gcacgatttt cccgctcgcg aagatgatca aggacgaagc gcggcgcatc
      121 gctgaagcga acaatattcc cgtcgcgcgc aagcccgact cgcaggagat ctgttttata
      181 cccgacaacg accacaaggc gttcctgaag cgcgagggtg tctcgatgcg gcctgggtat
      241 tttcgcgacc gtctgggcgc gatactcggc aaacatgaag gttcagtggg attaaccatc
      301 ggccagcgca agggcctcgg cgtggcgggt tttgccgaag cgcagttcgt gctcgaaatt
      361 ctctcgaatg gcgatgtcat tcttggcccc cgcgaagagc tcgagcgccg cacgttttat
      421 attcgcgatg tggttttcca gggcgtcgcc gaagacgacc gcgcgtggtc agaaggtgta
      481 ccgtgcaacg tgcagattcg ttacaacggg cagccgttgg cggcggtggc atacaaggta
      541 gagacgccat taatggcgtc tctacggatt gaattagata cccctgcatg ggcagtgaca
      601 cccggccagg cgggggtact ctatgaccgt gacggcgggt ttatcattgc cggtggcaag
      661 attaggctga atcaaggcgc taccgtccat gcagcagact gacctgagta ccagttttca
      721 tccaaaagcc tacctacacc tcgcgctcat cgtgatggcg agtctgttac tgctacctgt
      781 gcgaggcctg atcgtgcccc atctgagtgc aaatgaggtg tcaattgtca attgggtgct
      841 aggtggcgtt ctgggaatca cagcgctctc agtattcata accttgcgca agaattggat
      901 gacgtatgcg ctcgaaccag atgctatcgt gatcaggcac aggattctgg ctgaccggcg
      961 aatacctctg caagtcgttc attcagcacg aatggtggaa atccagggtg gcagaggata
     1021 taagtatcaa tcgatcgaac tacaattgca gagcggagat tttacaatac tggacgggct
     1081 ttacagtcgc ggtatttagg aattctacgg gggcacttgc gtctaagtta agataagcat
     1141 tgggttgcag ctacaaactg aaagcgagaa gaacaccaat atgccagcat atacctcact
     1201 caagctgaaa agcggactgc cgtggctggc tgctttcctc cttatgaccg cagtcgcgct
     1261 cttcttgcgt actcctgaaa cggttggcag gtacttgctg cttctcacaa tcgcctgggt
     1321 cgtgcttttc ttcggctggg cccgcaccac gcactcagcg tcgttacggc agattttgct
     1381 cttctccacg atcctgagtg tcttccaggt atttcccgat tgggtactct cgcggcatct
     1441 cggcgtactg gtctttccac ccgatggcct gttcaagtgg gacaccgtgt caggatacat
     1501 ggcgctactc tggacaatac ctgcctcgca gattctgatc gcctactcgg tcgcgcatga
     1561 gcgctggggc tataagctgg cctatgccgc cgcaatattg gtgggagcta ttcttttcat
     1621 cgggtctgaa gaactgcttt ggatgctgcc ctcttggtac gcaattaatg tgatcaaatg
     1681 gggccatgtc gcccgctata tcatcctgcc ggagatcttg ctgagcttca tgattgtttt
     1741 tgtatttgga cgcgtgaaag aaaaatctct cctgtggtat acctgcggcg cggccctggt
     1801 ttcatttatc tatacagcag cggccgtgtt gagctaccac ctgatgggat aattcgatgc
     1861 aacccctcaa agaccagttc ttcagcaaga catacattac cacgctcgca gccgatgtaa
     1921 aaaccgctgc gccagatttt gacgcggccg cattcacaag gcaggtactc gaccgcaaat
     1981 ggaaaaaccg cgagctcaaa gaccgcatgc accacgtcgc cgactgcctg cacgcagctc
     2041 tcaaccgtga ttacaaaagt tctatccgct tgctgacacc catcgtgcag cagaggatca
     2101 aagaaggcac caattcattc ggtgacatga tctttcccga ctttgtcgag cgttttggtt
     2161 tggaggattt caaaacttct gtcagggcgc tcgaagtgtt cacggctgcg tgcagttctg
     2221 aatttgcggt caggccgttt atcgttcgtt atccgcaaga gatgatgcgg cagatgctcg
     2281 aatggtcgaa gcacaaaagc gagcacgtac ggcgccttgc ttcagaaggc tgccgcccgc
     2341 gtttgccctg ggcaatggct ttgcccgaat tcaagaaaga cccgcgacct gtgctcaagg
     2401 tattggaaaa tctgaaatcc gacacatcag aatatgtcag gcgcagcgtc gccaataacc
     2461 tgaacgatat ttcgaaagat aacctcgagg tcgcgcttca gaccgcagaa cgctggttcg
     2521 gcaaaaacaa ggcaacggac gcgcttgtca ggcatgctct gcgcaacgag ctcaaaaaag
     2581 ggaataagcg cgcgctggcg ttatttgcct taccgcacag gacaaaggcc agaattaaca
     2641 aactggaaat tacacccgca agaatcagga ttggcgaatc ggtgcacatc cattttaccc
     2701 tcgaaagcac ggcaaccgaa cgcctgcgcc ttgagtactg ggtcgatttt gtgaaggcga
     2761 agggtcacgc gacccgaaag gttttccaga ttttcgaagg ggatttttcg gcgggtgcga
     2821 cgcgtgcctt tcgcaaaaaa caccgcttcc aggatttcac cactcgcaag cactatgcgg
     2881 gtgcacaccg catcgccatt gcggtgaatg gttcagccaa ggctgagacc gaggtgcatt
     2941 tggccgatta aagaccggcc tgttttacgg cgctggcgat ctcaggtgaa gtccagtcga
     3001 catcgccgac tttcgcatag atcgtctcac cgttcgcatt cacgatatac gtcgtcggaa
     3061 tcgcgtggat tccatacgcg ccgaaatcag cgggcaatgc gtagaagcga aatgggtgcg
     3121 cggtgcggta ttggtagcgc accattgcgg taatatcttc gtcggtgagc gcaatggcat
     3181 cgacgccgcg caggtatccg cgtaatgcct gcgccagctt tggcatttca cgcgcacaat
     3241 cgtggcacca gctcgcataa aatacgacaa ctgctccgcg gccgttctgt gaccagaccc
     3301 tggtgggtgc gcctttggca tccaacgcct ccagatcggt tatggccagc actggtgcga
     3361 tgcgatattt attgtacagc aggccacctg caaacagcaa caaaccgaga attgcagcca
     3421 gtccgatgcc gatggccttg gtagacacac tggtttctaa gccatgttct gttccctgtc
     3481 aaatctttat ccgcatggaa tgtgacggta agacagggtg cagcgccttc tcggctgaca
     3541 cagtgagcgg atatctgctt gaggttgcta ttagcccctc atggataacc tgacacattc
     3601 gatgactgct gcaatcgcgg caaagttcgt ggaccgcggc agcgaagaag ataagac
//
LOCUS       DAOQJF010000334         3642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16027_length_3642_cov_5.382214, whole genome shotgun sequence.
ACCESSION   DAOQJF010000334 DAOQJF010000000
VERSION     DAOQJF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16027_length_3642_cov_5.382214"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLF85_14395"
     CDS             <1..224
                     /locus_tag="PLF85_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20910.1"
                     /translation="KPCSAESRPQSQSFSAVASQSFARLDCTAHENPSLYSKASMIWY
                     RTPGSGSGQDLKLPESGFSHLEMGCYLRA"
     gene            290..1273
                     /locus_tag="PLF85_14400"
     CDS             290..1273
                     /locus_tag="PLF85_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNE20911.1"
                     /translation="MTHPNDALFAGEKPFPVIATCEHFAGNEKMIMKAFSFQEEKGPV
                     FDITQDCEDGAPTGQEKEHAEMIVRLTNSEHNKHNMAGARIHDYTSKYWKQDVDILVP
                     GCGERLAYITIPKPTAYEQVKEMIEYIQAAAKKAGLKREIPIHVLIETHGALRDVEKI
                     ATLPWLQVIDFGLMDFISGHHGAIALSNMKSPGQFDHKLLARAKSNCVAAALANGVVP
                     AHNVTLDLKNFEQTKSDASRARNEFGFLRMWSIYPTQIDAIVEAMKPDLSEVKKGVAV
                     LLAAQDAAWGPIQHDGELHDRATYRGFWTIVQRGRLTGIDIGAEATKRWFA"
     gene            1625..2248
                     /locus_tag="PLF85_14405"
     CDS             1625..2248
                     /locus_tag="PLF85_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20912.1"
                     /translation="MITVLSEKSAGIRLTSTMLPDGNFKLQFLFPQDASGILYKVTTV
                     LFAYGWDILSAYSRPNEEAEVEDIFIVSSTRHEALTKTACDQILAAVRELVAQETTPE
                     EFISRFPEKTQQIKNARAVKSDFMMAADTKHHCTKLVVERPDRPGVLMQITQVLYALG
                     LDIIAFSAEAVYQGVRDEFLVHHADGTEISEKDRVFLADLLNQKLTF"
     gene            complement(2311..3006)
                     /locus_tag="PLF85_14410"
     CDS             complement(2311..3006)
                     /locus_tag="PLF85_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20913.1"
                     /translation="MTRFALVGGGPSLDYCDRVIEDLAEAGCRFLVSDSVAAAFLRRW
                     PGLDAAVFTVELRRHPYLLRLSQAAPVYAYHKAHPRNLRLYQKGNIIPFKLSGEQGDI
                     PALYSPGTVLGTMLSFAAGSLGESGGEIHILGADLFYIDDQVYNRYIDPHAPPGNRLA
                     SREFWQYEMVLKKSSGVLAKSGFAIRTSFEFMQARENMRAFVRQLPENLHICEYSPLG
                     LDCEQVQKKNPVE"
     gene            complement(3049..>3642)
                     /locus_tag="PLF85_14415"
     CDS             complement(3049..>3642)
                     /locus_tag="PLF85_14415"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNE20914.1"
                     /translation="DIEYWDKIPTKKNYAAQKVRVVPPAVARYGSFLEPGAIMMPSYV
                     NIGAYVGSGTMVDTWATVGSCAQIGQNVHLSGGVGIGGVLEPPQGLPVIVEDHAFLGS
                     RCVVVEGVHVSKGAVLGSGVILTASTKIIDVSKSETQTYKSFVPRNAVVIPGSYAKKF
                     PGGEFQVPCALIIGERKESTDLKTSLNNALRDFEVSV"
BASE COUNT          863 a         1072 c          948 g          759 t
ORIGIN      
        1 gaaagccgtg ttcagccgaa tcccggcccc agagccagtc gttttcggcc gttgcgagcc
       61 aatcgtttgc ccggttggat tgcacggctc acgaaaaccc cagtctatac agcaaggcaa
      121 gcatgatttg gtaccgcacc ccgggcagcg gcagcggaca agatttgaaa ttgccggaat
      181 ctggattttc gcatctcgaa atgggctgtt atctgcgggc ttaagcccgt agttaacaaa
      241 ccagcccttt aattgacgct ctgtctcaaa ccccggtttt tcccttatta tgacacaccc
      301 caatgatgcc ctctttgcag gcgaaaaacc ctttcccgtg atcgctacct gcgagcactt
      361 tgctggtaac gaaaaaatga tcatgaaggc tttcagcttt caggaagaaa aaggccctgt
      421 tttcgacatc acccaggact gtgaagacgg agctccgact ggccaggaaa aagaacacgc
      481 agagatgatc gttcgcctca ccaacagcga gcacaacaag cacaacatgg ctggcgcccg
      541 tatacacgac tacaccagca agtactggaa acaagacgta gatattctcg tgccaggctg
      601 cggcgagcgc cttgcttaca tcacgattcc caagcccact gcttacgagc aagtgaaaga
      661 gatgattgaa tatatacagg cggctgcgaa aaaagcgggc ctcaagcgcg agatcccgat
      721 ccacgtgctg atcgaaaccc acggcgcact gcgcgacgtt gaaaagatcg cgacactgcc
      781 atggctgcag gtgatcgact tcggcctcat ggacttcatc tcgggccacc acggcgccat
      841 cgcgctctcg aacatgaaga gcccaggcca gttcgaccac aaactgctcg cacgcgcgaa
      901 atcgaactgt gtcgctgcag ctctcgcgaa cggcgtggtt cccgcgcaca acgtgacact
      961 cgacctcaag aacttcgagc aaaccaagtc tgacgcttca cgcgcccgca atgaattcgg
     1021 cttcctgcgc atgtggtcga tctaccccac gcagatcgat gccatcgtcg aagccatgaa
     1081 accagacctc tctgaagtaa aaaaaggcgt cgcggttctg ctcgcagcac aggatgctgc
     1141 atggggccct atccagcacg acggcgaatt gcacgaccgc gcaacctacc gcggtttctg
     1201 gacgatcgtc cagcgcggcc gtctcaccgg tatcgacatc ggtgcagagg cgacgaagag
     1261 atggttcgca taattcggcg cagctgaatt gtgcgaaggc cgaagcagct cgcagacgta
     1321 ccgtcttcgg cgctaccgga aagcagcgaa gcgctctagc agcaatttcg tcttcgaaat
     1381 tgcgtgaccc gcttgcgggt ctccgcctcg gaggctttcc ggtagcggtc tgcgagcgcg
     1441 aggcctaaat taatttcggt agcgtgtcac acacccttcg ggtgtgtgac cgcgcattcc
     1501 gctacggccc tacgggcccc gcttcaatcg ctcacgctcc ttctcagact atttcgaaac
     1561 ttcggtctgc aatcctcatc gattcccctc tccgcaaatg gcacgacgtt tgcttggtcc
     1621 ggttatgata actgtactct ctgaaaaatc cgccggcatt cgcctcacca gcaccatgct
     1681 gcccgatggt aacttcaagc tgcaattcct gtttccgcag gatgcaagcg gcatactcta
     1741 caaggtgacg acggtgctgt tcgcctacgg ctgggatatc ctgagcgcct attcgcgacc
     1801 gaacgaagaa gctgaggttg aagacatctt catcgtcagc tctacgcggc atgaagcgct
     1861 gacgaaaaca gcctgcgacc agatactggc tgcggtcaga gaacttgtgg cgcaggaaac
     1921 tacgccagag gaattcattt cacgcttccc ggaaaagacc cagcagatca aaaatgccag
     1981 ggcagtcaaa tcagacttca tgatggcagc tgataccaaa caccactgca ccaagctcgt
     2041 ggtcgaaagg cccgaccggc ccggggtact catgcagatc acgcaggtgc tctacgcact
     2101 cgggctggat attatcgctt tctccgccga agctgtctac cagggcgtgc gcgatgagtt
     2161 tttagtgcac catgcggatg gaaccgagat cagtgaaaaa gacagggtat ttctggcaga
     2221 cctgctgaac cagaagctga ctttctaaca gtttcctgaa aaaggaaact gatagcaggt
     2281 ttccggaacc tgctagcggg cagctccccg ctattcgact ggattttttt tctgtacctg
     2341 ctcgcaatcc aagcccagcg gtgaatactc gcagatatgc aggttttccg gtagctgccg
     2401 gacaaaggcg cgcatgtttt cccgggcctg catgaattca aaggaagtgc gtatggcaaa
     2461 acccgatttt gccagaacac ccgagctctt cttcagtacc atctcgtatt gccagaattc
     2521 gcggctggca agacgattac caggtggtgc atgcgggtcg atgtagcggt tgtagacctg
     2581 gtcatcaatg tagaagagat ccgctcccaa aatgtgaatt tcgccgccac tttcaccgag
     2641 gctgcccgcg gcaaaagaca gcatagttcc cagtactgtt cccggtgaat acaaggccgg
     2701 aatgtcaccc tgttctcctg agagtttaaa cgggataata ttaccctttt ggtacaacct
     2761 gagattgcgt gggtgcgcct tatggtacgc atagacgggt gcagcctgcg aaagacgcaa
     2821 aaggtacgga tgccgccgca attcgactgt aaagacggcc gcatccagac cgggccagcg
     2881 gcgcaaaaaa gctgctgcca ccgaatcgct gaccagaaag cggcagcccg cctctgcaag
     2941 atcttctatt acacgatcac aataatcgag cgatgggcct ccaccgacga gagcaaacct
     3001 agtcaatggc ggcaccgctg ggtcgatggt cgatgcttct tcgttgggtc agacgctcac
     3061 ttcgaaatcg cgcagggcat tgttcagcga ggtcttcaga tcggtcgact ctttccgctc
     3121 gccgatgatc agtgcacacg gcacctggaa ctccccgccg ggaaatttct tggcgtaaga
     3181 accgggaata acgaccgcgt tacgcgggac gaaacttttg taagtctgcg tctctgattt
     3241 tgagacatcg atgatcttgg ttgaagccgt gaggataact ccgctaccca gaacagcgcc
     3301 tttactcaca tgcactcctt cgacgacaac acagcgtgac ccaagaaagg cgtggtcttc
     3361 cacgatgaca ggcaggccct gcggcggctc aagcactcca ccgattccga ctccgcccga
     3421 gaggtgcaca ttctgcccga tctgcgcgca gctgcccacg gtcgcccagg tgtcgaccat
     3481 cgttccggaa cctacgtacg ccccgatgtt gacatacgat ggcatcatga ttgcccccgg
     3541 ctccaaaaag ctgccgtagc gtgccactgc cggcggtacc acacgcactt tctgcgcagc
     3601 atagttcttt ttcgtgggga tcttgtccca gtactcgata tc
//
LOCUS       DAOQJF010000335         3642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16028_length_3642_cov_4.643713, whole genome shotgun sequence.
ACCESSION   DAOQJF010000335 DAOQJF010000000
VERSION     DAOQJF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16028_length_3642_cov_4.643713"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLF85_14420"
     CDS             <1..327
                     /locus_tag="PLF85_14420"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNE20915.1"
                     /translation="WVGLKGDGDWAGLLPEGYALPVSHGEGNFICSDEELKAIRDNGQ
                     AILRYTNNPNGATFDIAGVASANKRVIGLMPHPERALRVQPDAAVSRERYGRKFFEKI
                     FSMIPG"
     gene            329..1330
                     /gene="rsgA"
                     /locus_tag="PLF85_14425"
     CDS             329..1330
                     /gene="rsgA"
                     /locus_tag="PLF85_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNE20916.1"
                     /translation="MANERALVAGVFGAFYHVLLFTEEDGRRIYGERRLAKTRGKLRI
                     DYREKNTGYNKLRETLLAIGDVVEVGVDAKNPETMVIIDVEKRHNAIHRASFGRQQCL
                     AANIDGVVIMSSVRSPDFNLGFVERCLAEVTIARADVTLVVNKYDLAAEGDEETEKIL
                     LYYEKCGFSVFRESVLGGISEALAKHLESGTWLLLGQSGTGKSTLMNRLLGDERQIVG
                     EVSQIDKGRHTTTNPALHFSPRHAQLVLIDVPGLREFGLHHRDAQEIRTGYPDFLEHP
                     CRFENCLHLNEPGCGVKDAIASGELPEFRYRYYVSMLDTVQENFKPRKGDYWRGIRK"
     gene            complement(1357..2034)
                     /locus_tag="PLF85_14430"
     CDS             complement(1357..2034)
                     /locus_tag="PLF85_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20917.1"
                     /translation="MSSALRITGRLPAIREYLLQAIFPEPCLHCKSRLPENGLICPRC
                     LADWPQGEQNSTVPHSHYQTQYLYPYEGVQKSLFKAAKFSGRRRAQGFVFRKARAALQ
                     SLVQPGTVFLPMPSSRSFLSGLLQSAVPRAQIHPGMYLFQRGKWGDSNKHLGEAARFR
                     RIRESLVRSPKQLAVAERFVLCDDVLTTGATLGQAAWLLESGGVPREKIVLWALLYRR
                     RENDSLL"
     gene            complement(2043..2669)
                     /locus_tag="PLF85_14435"
     CDS             complement(2043..2669)
                     /locus_tag="PLF85_14435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HNE20918.1"
                     /translation="MIFSLTGVPVHVEPFFAVLECGGVGYGARIPVNVYQAIVAGAYD
                     TGKKPFSLVTRSVYSEDNAQLFGFLDQAEAQLFDFIRSLHGFGPQAAMNLISTIGGAE
                     LLQALKESDVKALTKVPGVGKTRAEKLCFEANSKKARLDKLSEKTGKQAAATATQDDL
                     LVQALVSLGYSQKEIDQAAEKIRKLPDLPSATTRENLQDWIRLYLRNL"
     gene            complement(2715..2945)
                     /gene="csrA"
                     /locus_tag="PLF85_14440"
     CDS             complement(2715..2945)
                     /gene="csrA"
                     /locus_tag="PLF85_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNE20919.1"
                     /translation="MLVLARRVNDSIVIGDNVEIVVVEIKGDQVKLGVKAPKDVKVFR
                     GEIHAEIQKENKAASESQLPPNLDSIIRKPKP"
     gene            complement(2945..3415)
                     /gene="fliW"
                     /locus_tag="PLF85_14445"
     CDS             complement(2945..3415)
                     /gene="fliW"
                     /locus_tag="PLF85_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNE20920.1"
                     /translation="MQFETRALGKIEIDASLQIYFPEGIFAFENYRHFALLPTKSGST
                     FHWLQSLEESQLAFLITRVPEIAPHYKPNVATEFLSLIEAVDLAKTEIWGIITVPAGK
                     PDQMTINLQGPVLINRERKLGGQFVSDDPAHRVRTPLLDLVEGPSTGATGAGQG"
BASE COUNT          792 a         1027 c         1031 g          792 t
ORIGIN      
        1 tgggtcgggc tcaaaggcga cggtgactgg gccgggcttt tgcccgaagg ttacgcactg
       61 ccggtctcgc acggcgaagg taattttatc tgctccgacg aagaactcaa ggcaatccgc
      121 gataacgggc aggcaatact gcgctacacg aataacccca acggtgcgac ctttgacatt
      181 gcgggcgtcg cttcggcaaa caaacgcgtg atcggcctca tgccgcaccc cgaacgcgcg
      241 ctgcgcgtac aacccgatgc ggcggtgtca cgcgaacgct acggccgtaa atttttcgag
      301 aagatcttct ccatgattcc gggctgatat ggcgaacgag cgggcactgg tcgcaggtgt
      361 ttttggcgcc ttctaccacg tgctgctctt caccgaagaa gacggccggc gaatctacgg
      421 cgaacgccgc ctggccaaga cgcgcggcaa actgcgcatc gactaccgcg aaaaaaacac
      481 cggctacaac aagctgcgcg agacgctgct ggcaatcggc gacgtggttg aagtgggcgt
      541 cgacgccaag aaccccgaga ccatggtaat tatcgatgtc gaaaaacgcc ataacgcgat
      601 acaccgggca tcttttggca ggcagcagtg cctcgcagca aacatcgacg gtgttgttat
      661 catgtcgagc gtgcgctcac cggattttaa cctgggcttc gtcgaacgtt gcctggccga
      721 agtcactatt gcccgcgccg acgtcacgct ggtcgtcaac aagtacgacc ttgctgccga
      781 aggcgatgaa gagacagaaa agattctgct ctactatgaa aaatgtggct tcagtgtctt
      841 ccgcgagagt gtgctcggcg gtatcagcga agcgctggca aagcacctcg aatcgggcac
      901 gtggcttctg ctggggcagt ctggaaccgg caaaagcacc ctgatgaacc ggctgctcgg
      961 cgatgagcgg cagatagtgg gagaagtaag ccagatcgac aagggccgcc acacgacgac
     1021 gaatcccgcg ctgcacttca gtccacgcca cgcgcaactg gtgctcatcg acgttccggg
     1081 cttacgcgag tttggcctgc accaccgcga tgcgcaggaa atccgcacgg ggtaccccga
     1141 tttcctcgag cacccctgtc gcttcgagaa ttgcctgcac ctcaatgaac cgggctgcgg
     1201 agtgaaggac gcgattgcca gtggcgaact gcccgagttt cgttaccgct attacgtttc
     1261 gatgctcgat accgtgcagg aaaattttaa gccgcgcaag ggtgattact ggcgggggat
     1321 tcggaaatag tctagcggct cctgataagg aacctgctaa agtagactat cattctcgcg
     1381 tcgacggtag agcagcgccc acaggacaat tttttcgcgc ggcacaccac cgctctcgag
     1441 cagccatgca gcttgcccca gcgtcgcacc cgtggtcagc acatcatcgc ataagacaaa
     1501 acgctctgcc acggcaagct gcttcgggct gcgcaccagg ctttcgcgga tacggcggaa
     1561 gcgagctgcc tcgccgagat gtttattgct gtcaccccac ttgcctcttt gaaaaagata
     1621 cattcccgga tgaatctgtg cgcgcggcac agctgattgc agaagcccgc tcaaaaaact
     1681 gcggcttgat ggcatcggca aaaacacggt cccgggctgt acgagcgact gcagtgcggc
     1741 acgcgcctta cgaaacacga aaccctgcgc gcgccgacgc ccggagaatt tcgctgcttt
     1801 aaagagactc ttctgcaccc cttcgtaagg gtaaagatac tgcgtctggt agtgcgaatg
     1861 gggaacggtg ctgttctgct caccctgcgg ccaatcggcc aggcaacgcg ggcagataag
     1921 tccattttca ggcaggcgcg atttgcagtg caggcagggt tcgggaaaga ttgcctgcaa
     1981 caggtactcg cgtattgcag gcaggcgtcc tgtgatgcgc aaggcggagc tcatgagttc
     2041 agttaaagat tacgcaggta gagcctgatc cagtcctgca ggttttcacg cgtggtggca
     2101 gacggcaggt cgggcaattt acggatcttc tcagccgcct ggtcgatttc tttctgtgaa
     2161 taaccgagcg aaacaagcgc ttggacaagc agatcatctt gtgttgcagt tgctgctgcc
     2221 tgcttgccgg tcttctcgct gagtttgtca agccgcgctt ttttcgaatt ggcttcgaag
     2281 cagagttttt cggcccgggt cttgcccacg ccgggcactt tcgtcagcgc cttgacatcc
     2341 gactctttga gcgcctgcag cagttcggca ccgccgattg tggaaatcag gttcatcgct
     2401 gcctgaggac cgaagccgtg cagcgagcgg ataaaatcga ataactgcgc ttcggcctgg
     2461 tcgagaaaac caaaaagctg cgcattgtct tcagagtaga cgctgcgcgt cacgagtgaa
     2521 aagggcttct tacccgtatc gtaagccccg gcgacaatcg cctggtagac attgaccggt
     2581 atgcgcgcac catagccaac tccgccgcat tcgagcacag caaagaaagg ttcaacatgc
     2641 accggaacgc cggttagcga gaagatcatg cgtgtctctg ccgcttaaat cagggcgggc
     2701 aagtgttttt cagcctaggg tttaggcttg cggataatac tgtcgaggtt cggtggtagc
     2761 tgcgactctg aagccgcctt gttctctttc tggatttctg cgtgtatttc gccgcgaaaa
     2821 actttgacgt ctttcggcgc tttcactccc agcttgacct ggtcaccttt aatctcgaca
     2881 acaacgatct cgacgttgtc gccgatcacg atcgagtcat ttacccggcg cgcaagtacc
     2941 agcatcagcc ctgccctgcg cccgtcgcac ctgtcgaagg cccttctacc aggtctaaaa
     3001 gaggagtccg cacgcggtgc gcggggtcgt ctgagacaaa ctggccaccc agtttgcgct
     3061 cacggttaat cagcaccggc ccctgcaggt taatggtcat ctggtcgggt ttgccggcag
     3121 gaacagtgat aattccccag atttctgttt tggcgagatc gacggcctcg atcagcgaaa
     3181 ggaattcagt tgctacgttt ggcttgtagt gcggggcaat ctcgggtaca cgcgtgatga
     3241 gaaatgccag ttgcgactct tccaatgact gcagccaatg aaaggtactg ccggattttg
     3301 tcggcagcaa ggcaaagtgc cggtaatttt caaacgcgaa tatgccttcg ggaaaataga
     3361 tctgcagcga tgcgtcgatc tctattttac ccagcgcgcg tgtctcaaac tgcatgtgac
     3421 tcctgaaatt tcctgtctgc tattcgtcta cgttgcttgt tggtcaccgc agaaaatcca
     3481 tcagggtttg tttgatgata ccggcaccca cgcgcagcgc gtacgagtgc acggcttcga
     3541 gccacttcag gtcaacgatt gcctgggtaa catcgacgct ttcattcttg gcgagcaatt
     3601 ccttggtgta tgtggtgtcc cacgcaatac gcttttcgtg ct
//
LOCUS       DAOQJF010000336         3640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16042_length_3640_cov_3.841562, whole genome shotgun sequence.
ACCESSION   DAOQJF010000336 DAOQJF010000000
VERSION     DAOQJF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16042_length_3640_cov_3.841562"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLF85_14450"
     CDS             complement(<1..397)
                     /locus_tag="PLF85_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20921.1"
                     /translation="MRRLLLLPFFLLACREKAAQLVAAHDSATTAFALAADRQVNGKL
                     HSGDNQVYFSFRLPEQVMFRAELSAVRGADTQLEILSARGEVLFTANDHGSSLAEEIH
                     PVFLAAGDYLLRLSANAELAEDFTLVYRLF"
     gene            complement(397..672)
                     /locus_tag="PLF85_14455"
     CDS             complement(397..672)
                     /locus_tag="PLF85_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20922.1"
                     /translation="MDTLESIETQSANEPPDYIFDLIQVSLLAAAHDIPGLIAAVDKA
                     RAAGADNREILRAMFRSDDAFVAGIENAEFLRLAESLQAGQVPVLSA"
     gene            819..1082
                     /locus_tag="PLF85_14460"
     CDS             819..1082
                     /locus_tag="PLF85_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20923.1"
                     /translation="MKKANLLMGAALAGLALAGAAQAKEGKKAAKEGECHGINGCKGQ
                     GDCGNKDHGCGGQNACKGQGWKKMTEAQCKAKKGKFKPLSMEM"
     gene            1109..2686
                     /locus_tag="PLF85_14465"
     CDS             1109..2686
                     /locus_tag="PLF85_14465"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNE20924.1"
                     /translation="MHKNLVGLGLRTEHFNHLLEKPKTGIGWFEIITENFTDTAGAPL
                     YVLEQLRKDYPVAFHGVALSIGSVDGARREHLQRIRELAERIEPFQVSDHFCFARFGS
                     RQYHELLPLPRTAAMAARVIANIRQVQDFLKRQLVLENISAYLEYPHNEMSEAEFINY
                     VAEKSGCGVLLDVNNIYVNAANFRYNARRAILAFDPKHIVQYHLAGFTDLDTHLFDTH
                     AETVHAPVRQLFRETRHHLGERRFSVERDDKIPTFGRLERETLSLAALSPQPMPKRFL
                     SPAPAYLPAGDFRRRRGLRHEKDWQRRIYDKEGSKAERSAGSLTVKAAREVYRNAYNI
                     RFTGALRDKFGHLAAFMTERALERLFADYVKETPSVHEDLGAYGASLPDFLKKKLPQR
                     SYLADAARLDLLRYELFHRTVRPAKKEILRQKVKLQGACLWRSNFTLFSGARNGRLHK
                     APGKPRKKAQYAIVYRKAFAVHERVLSVAQYDFVAALEKGARVPTLLAQAESARTMTA
                     IEIRQLFEVLGLEGVLV"
     gene            2748..>3640
                     /locus_tag="PLF85_14470"
     CDS             2748..>3640
                     /locus_tag="PLF85_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20925.1"
                     /translation="MKSARLTNSLWFGICALAIAWLLVTGTPYENTLWLAATGKVDLG
                     LSLFYRFRDSTIVTDGTWGGPGLGIFLGVCSLIGVRAAWSVTIVLQAFLLHQKSLKIK
                     IFSAALLVAALLWGEALLVWNLLFTLLVHVFGRIAAPRNLRAVLITLVALAGFLGAVE
                     LPWVMGNPARSVTRQRVLGGKSHFDVLKPQLRRPAIILHESCLAVLNPDDLRGVQSTE
                     LLSRSSLANFEAVILLDLKIVEMNYRFLTMKDEKGEFVFSNAARNFAEKEKAHAAAVR
                     ARLESAKNLCLIAPFDLLPDQ"
BASE COUNT          836 a         1062 c          999 g          743 t
ORIGIN      
        1 tgaacaggcg atagaccagc gtgaaatctt cggccagttc ggcatttgcc gataagcgca
       61 gcagatagtc gccggcggcc aggaacaccg ggtgaatttc ttcggcaagg gaagagccgt
      121 ggtcgttcgc ggtgaacagc acttcaccgc gtgcactcag tatttcaagc tgtgtgtctg
      181 ccccgcgtac cgctgaaagc tccgccctga acataacctg ttctggaagg cgaaagctga
      241 aataaacctg gttgtcgcca ctgtgcagct taccatttac ctgccggtct gcagccagcg
      301 caaaggcggt tgtggcagag tcgtgggcag caacaagctg cgctgccttt tcacggcacg
      361 cgagcagaaa aaacggaagc agcagaagcc ggcgcatcag gccgacagca ccggcacctg
      421 gcccgcttgc agcgattcgg caaggcgcag aaactcggca ttctcaatac ctgcaacgaa
      481 tgcatcgtcg ctgcgaaaca tggctcgcag aatttcccgg ttatcggcgc ccgcagcgcg
      541 tgctttatcc accgcggcga tgagcccggg aatatcgtgc gccgccgcta aaagagagac
      601 ctggatcagg tcaaaaatat aatcgggcgg ctcattcgca ctttgagttt cgatggactc
      661 gagagtgtcc atgcggactt ttattgcgcg ctgcacaaat cgtcaatcgc aatacaggta
      721 gtttttgaca gctcatctgt ctaaccatca gcttcccgca gaagaaagcc ggggctgacc
      781 agcaattcag gcgcgcagcc ccaaaggaga atagagcaat gaaaaaagca aacttactca
      841 tgggtgccgc actggcaggg ctggcgcttg ccggcgcagc acaggcaaaa gaaggcaaga
      901 aagccgcgaa agaaggcgaa tgccacggca tcaacggctg caagggccag ggtgactgcg
      961 gcaataaaga ccacggctgc ggcggccaga acgcctgcaa aggccagggt tggaaaaaaa
     1021 tgaccgaagc gcagtgcaag gctaaaaaag gcaaattcaa accactgtct atggagatgt
     1081 gagttcaccc tgaacgggct gaacccacat gcacaaaaat ctcgtcgggc tcggtcttcg
     1141 gaccgagcac tttaaccacc tgctcgaaaa accaaaaacc ggcatcggct ggtttgaaat
     1201 catcacggaa aattttaccg ataccgcggg cgcgccgctc tacgtgctgg agcagctgcg
     1261 caaagattac ccggtcgcct ttcacggcgt agcgctttcg atcggcagcg tcgacggcgc
     1321 caggcgagag cacttgcagc gtattcgtga gctggcagag cgtatcgagc cattccaggt
     1381 atctgaccac ttctgctttg cgcgttttgg ctcccgccag taccacgagc tgctgccgct
     1441 gccgcgtaca gctgcaatgg ctgcgcgcgt cattgccaat atacgccagg tgcaggattt
     1501 cctgaagcgg cagctggtgc tcgaaaacat ctcggcctac ctcgagtacc cacacaacga
     1561 gatgagcgag gcagagttca tcaactacgt cgctgaaaaa agcggctgcg gagttctgct
     1621 cgatgtgaat aatatttacg tcaacgcggc caacttccgc tacaatgccc gccgcgcgat
     1681 tctggcattc gacccgaagc atatcgtaca gtaccacctc gctggcttca ccgacctcga
     1741 cacgcatctc tttgatacac acgccgagac cgtacacgcg ccggtgcgcc agctgttccg
     1801 cgaaacgcgg caccacctcg gcgagcgccg tttcagcgtt gaacgcgatg ataagatacc
     1861 gacatttggc agacttgaac gcgagactct ctcactggca gcactttcgc cgcaaccgat
     1921 gcccaaacgt tttttatccc ctgcccccgc ctatttgcca gcgggggatt ttcggaggcg
     1981 ccgaggcttg cggcatgaga aagactggca acgccgtatt tatgataaag aaggctcgaa
     2041 agcagaacgc agcgccggca gcctgacggt caaagcagcc cgcgaggtct atcgcaatgc
     2101 atacaatatt cgcttcaccg gcgcactgcg cgacaagttt ggacacctgg cagcctttat
     2161 gaccgaacgc gcgctcgaac gactttttgc tgactacgtt aaagaaacac cctcggttca
     2221 cgaagatctc ggcgcctatg gcgcctcgct gcctgacttc ctgaagaaga agctcccaca
     2281 aaggtcttac ctcgccgacg cagcccggtt agacctgctg cgctatgagc tattccatcg
     2341 cactgtcagg cccgctaaaa aagaaattct gcgccaaaaa gtaaagctgc agggcgcatg
     2401 cctgtggcga agcaatttta ctcttttctc gggtgcgcgt aatggtcgcc tacacaaggc
     2461 accgggtaag ccgcgaaaga aggcgcagta cgccattgtc taccggaagg ccttcgcggt
     2521 gcacgagcgc gttctttcgg ttgcgcaata cgactttgtt gctgcgctcg aaaagggggc
     2581 tcgcgtgcca acgcttctgg cgcaagccga atctgccaga acaatgactg caattgaaat
     2641 acggcagctg ttcgaggtgc tcggcctcga aggggtactg gtgtgaaggc agctcatctg
     2701 ctcgcggcaa tccggaaaaa tatccgccgc agaatcggag caggcgagtg aaatctgcac
     2761 gcctcacgaa ttcgctgtgg tttggcatct gcgcgctcgc catcgcctgg cttctcgtga
     2821 caggcacacc gtacgaaaat acgctctggt tggccgcaac gggtaaagta gatctcgggc
     2881 tctcactttt ctaccgcttt cgcgacagca ccatcgttac cgacggcaca tggggaggtc
     2941 cgggcttagg catctttctc ggtgtctgct ccctgattgg agtacgcgct gcgtggtcgg
     3001 tgactatcgt attgcaggct tttctgctgc accagaaatc tctgaagatc aaaatcttct
     3061 ccgccgcgct gctggttgcg gcattactct ggggcgaagc actgctcgta tggaatttgc
     3121 tgtttactct tctggtccat gtattcggta gaatcgccgc accccgcaac ctacgggctg
     3181 tcctgatcac actcgtcgca ctggcaggct ttctcggcgc cgtcgagctg ccctgggtaa
     3241 tgggcaatcc cgcacgctcg gtgacccgcc agcgtgtgct cggaggcaaa tcccactttg
     3301 acgtgctgaa gcctcagctg cgtcgtcccg ccattattct gcacgagagc tgccttgccg
     3361 tgctcaaccc cgatgacctg cgcggcgtgc agagtaccga acttctgtcg cgctcgtcgc
     3421 tggctaattt tgaagcggtc attctgctcg acctcaagat cgttgaaatg aactaccgat
     3481 tcctcacgat gaaagatgaa aaaggggagt ttgtattcag caacgccgcg cgcaatttcg
     3541 ctgaaaagga aaaagctcac gcagccgcag tgcgcgctcg ccttgaatct gcgaaaaacc
     3601 tttgtctcat agctcctttc gatcttttgc ccgatcagga
//
LOCUS       DAOQJF010000337         3638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16050_length_3638_cov_4.870779, whole genome shotgun sequence.
ACCESSION   DAOQJF010000337 DAOQJF010000000
VERSION     DAOQJF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16050_length_3638_cov_4.870779"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /gene="mreC"
                     /locus_tag="PLF85_14475"
     CDS             complement(<1..766)
                     /gene="mreC"
                     /locus_tag="PLF85_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNE20926.1"
                     /translation="MLAEILSRYKTGSSLVFTIVFSLSCLIWKSNLLSRTANSASQAL
                     DFFSSTFSTFGKGMSRFVDSYGTYETVRAERDALREKVKSNLDLQVELVRLREENNKL
                     RQALQLPPVSNYPVLQAEVISQDPDNWFRTIIINKGSADGIATYMPVVALQTITLPAE
                     AGKPAETKLVVGIVGKVIQVNPHSARILPLTDQFSRIGVNVKKTGHWALLNGQSPHQD
                     APLLDYLSLGVFISPGDEIVTSGGDGIFPRGLPVGIV"
     gene            917..1306
                     /gene="apaG"
                     /locus_tag="PLF85_14480"
     CDS             917..1306
                     /gene="apaG"
                     /locus_tag="PLF85_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNE20927.1"
                     /translation="MPTTASSEIVTDGIRVRAEPHYLPDRSDPENSQYLFTYRITLSN
                     EGPEWAKLRARHWIIIDSDGKREDVYGPGVVGEEPELDPGASYSYSSFCPLGTNFGTM
                     EGSFSMVRRDGTPFEIRIARFYLTTNE"
     gene            complement(1343..1873)
                     /locus_tag="PLF85_14485"
     CDS             complement(1343..1873)
                     /locus_tag="PLF85_14485"
                     /inference="COORDINATES: protein motif:HMM:NF022605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HNE20928.1"
                     /translation="MGSITRYIGSFFGSLAERLVVFFVALFLSQAPQYMNLYLNVLSG
                     AKAAHEKSVATLTEKAQELDMTVQQFIDDLLKADSKVSKSSGQVHQKQLADFENTKRA
                     FEALKNATAFSRPFVFIKHVDWSLAKNVQFQPAFPLTLEALMYVLVGIILGMILYRAL
                     MFFPRRLLGNKSAPAY"
     gene            complement(1932..>3638)
                     /locus_tag="PLF85_14490"
     CDS             complement(1932..>3638)
                     /locus_tag="PLF85_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNE20929.1"
                     /translation="RIAFLEQNLPVGETETAVQIVIGGDRALAKALKEREDPNLDPVH
                     AGEIEEVILSHDGYRAESEAARLLSGLGLPNEVHNIPAQKLSGGQMMRVLIARALFAK
                     GDILLLDEPTNHLDMPGIKWLEHFLLYEYRGMLLLITHDRGFLSALSEQILDVDYKTI
                     TLYNMNYNRFVGARLLAAEQKEREADAAKRKIAELQEFVDRFKAKASKARQAQSRVKQ
                     IEKIDVPEIIPSSRRKPSFFFRYEREPGRDILNVHGVAMGFTDEMLFSDAEFQLRKND
                     KAAIVGPNGHGKTTLLKIIAGELTPRAGTVHFGHEVRMGYYAQVHEDLRKLPTPMYEH
                     LYDQHSTKPIKDVRTALGHMLFEGDEQKKKLSALSGGEMARYVFADLMLQKPNFLVLD
                     EPTNHLDIEGIEALEDALADYKGTVLMVSHDRAFIKRVCNRVFELKNGSLQELMGGVS
                     ALDIFEDEADKPAPTARQKTEAAMAAEIVAPQKELSYEERKRLKSEAGKMQTRIKKLE
                     EEIARFETELKGIDALFADGSQLQALGADQLRLKTEERAAKQKALEECMAEWEKLSAV
                     NS"
BASE COUNT          782 a         1027 c          987 g          842 t
ORIGIN      
        1 cgactatccc cacgggcaag ccacgtggaa agataccatc cccacccgat gtgacaattt
       61 catcgcccgg cgaaataaac acaccgaggc tcaggtagtc gagtagcggc gcatcctggt
      121 gcgggctctg cccgttcagc agggcccagt gcccggtctt tttcacgttc acgccgatgc
      181 gcgaaaactg gtcggtcagc ggcagaatac gcgcggaatg cggattcacc tgtatcacct
      241 taccgacaat gccaacaacc agcttcgtct ctgcaggctt gcctgcctca gccggcaggg
      301 taatcgtctg cagcgcgacg acgggcatgt atgtcgcaat gccgtctgca ctgcccttgt
      361 tgatgatgat agtgcggaac cagttgtccg gatcctgtga aataacttct gcctgtagaa
      421 cggggtagtt cgaaaccggc ggtaactgca gcgcctggcg gagtttattg ttttcttcgc
      481 gcaggcgtac gagctcgacc tgcaggtcga ggttcgactt cactttttcg cgcagcgcat
      541 cgcgttcggc gcgcacggtc tcgtacgtgc cgtagctatc gacgaagcgc gacatgcctt
      601 taccgaaggt cgaaaaagtg ctggagaaaa agtcgagcgc ctggcttgcg ctgtttgcgg
      661 tgcgcgacaa caggttagat ttccagatca ggcagctcag cgaaaaaaca atggtgaaga
      721 caagcgatga gccggtcttg tagcgggata atatctctgc gagcatagct taaacggcaa
      781 aaccggccgt aggtattatg tctcttcatt atacagacgc tccgtcaatt tttttcaggt
      841 ctgcaggcct cgtttgggtt tcgcagctat ctgacgaaaa tcatttacgc cgcgttatca
      901 cccctcagcc acactcatgc cgacgactgc gagctctgaa atcgtcaccg acggcattcg
      961 cgttcgcgcc gagccgcact acctgcccga caggtcagac ccggaaaaca gccagtacct
     1021 gtttacctac cgcatcacgc tcagcaacga gggccccgaa tgggcaaaac tgagggcgcg
     1081 ccactggatt attatcgatt cagacggcaa acgcgaagat gtctatggcc cgggcgttgt
     1141 cggcgaagaa cccgaactcg acccaggtgc cagctattcg tactcgagct tctgcccatt
     1201 gggcacaaat ttcggtacca tggaaggcag cttcagcatg gtgcgccgcg atggcacacc
     1261 gtttgaaatc cgcattgcgc gcttctacct cacgacaaac gaataaaacc aggtgggagc
     1321 tcgtgcgtga cgagccggca catcaatacg ctggtgccga cttgttgccg agtaagcggc
     1381 gcggaaaaaa catcagcgcg cggtagagaa tcataccaag gataattccc accaggacat
     1441 acatcagcgc ttccagcgtc aggggaaatg ccggttgaaa ctgcacgttc tttgccagcg
     1501 accaatcgac gtgtttgata aaaacgaaag gccggctaaa ggctgtcgca ttcttgagtg
     1561 cttcgaaggc acgtttagtg ttctcgaaat cagccagttg tttctggtgc acctgacccg
     1621 aactttttga aaccttactg tctgccttaa gcaggtcgtc gatgaactgc tgcacggtca
     1681 tgtcgagttc ctgtgctttt tcggtcagtg tcgctacgga tttctcatgc gccgcctttg
     1741 cgccgctcag tacattgagg tacaggttca tgtactgcgg cgcctggctc aaaaacagag
     1801 cgacgaaaaa gacgaccagg cgttcggcca gcgagccgaa aaaagaaccg atataacgag
     1861 tgatgctacc catttgcccg cgtacaccga agcgcagcgc tgtaaagcga aagggggagt
     1921 gaatttgcct gctagctatt caccgctgag agcttctccc actccgccat gcactcttca
     1981 agggctttct gcttagcagc acgctcttcc gttttcaggc gcagctggtc ggcacccaac
     2041 gcctgcagct gtgaaccgtc ggcaaacagg gcatcaatac ccttgagctc cgtttcgaac
     2101 cgggcaattt cttcttcgag cttcttgatg cgggtttgca tcttgcctgc ttcgcttttc
     2161 agacgcttgc gctcttcata gctgagttct ttctgcggtg cgacaatctc tgcggccatc
     2221 gcggcctcag tcttttgccg cgctgtcggc gccggtttgt cagcctcatc ctcaaaaata
     2281 tcgagggcag aaactccacc catgagctcc tgcaggctgc cattcttcag ttcgaaaacc
     2341 cggttgcaga cccgcttgat aaaagcacgg tcgtggctca ccatgagtac agtgccttta
     2401 tagtcggcga gagcatcttc gagcgcttca atgccttcga tgtcgaggtg gttggtcggt
     2461 tcgtcgagca cgagaaaatt cggtttctgc agcatcaggt cggcaaagac gtaacgcgcc
     2521 atctctccac ctgaaagcgc actgagcttt ttcttttgtt catcgccctc gaacagcatg
     2581 tgtcccagcg cggtacgcac atctttgatt ggcttggtcg agtgctggtc gtataagtgc
     2641 tcatacatgg gcgttggcag tttgcgcaga tcttcgtgca cctgcgcata ataccccatg
     2701 cgcacctcgt ggccaaagtg cacggtaccg gccctggggg tcagctcacc tgcaataatc
     2761 ttgagcaggg tagttttacc gtgaccgttc ggaccgacaa ttgctgcctt atcattcttg
     2821 cgcagctgaa actctgcgtc gctgaagagc atctcgtccg tgaagcccat ggcaacaccg
     2881 tgaacgttta gtatgtcccg gcccggttcg cgttcatagc ggaagaagaa cgaaggtttg
     2941 cgtcgcgaag acgggataat ctctggcaca tcgatctttt cgatctgctt cacgcgtgat
     3001 tgtgcctggc gcgccttaga ggctttggct ttgaagcgat cgacgaattc ctgcagctcg
     3061 gcgatcttgc gctttgctgc gtccgcttca cgctctttct gctctgcagc tagcagtcgc
     3121 gcaccgacaa agcggttgta gttcatgtta tagagcgtga tggtcttgta gtcgacgtcg
     3181 agaatctgct cggaaagcgc ggacaagaaa ccgcggtcgt gcgtaatcag cagcagcatg
     3241 ccgcggtact catagagaag aaaatgttcg agccacttga tacccggcat gtccaagtgg
     3301 ttggtcggtt cgtcgagcag caggatatca cccttcgcga aaagggcgcg tgcaatcagc
     3361 acacgcatca tctgcccacc tgatagtttt tgggcgggta tattgtgcac ctcgttgggt
     3421 aatcccaacc ccgagagcag gcgcgcagct tctgattcag cacggtagcc atcgtgcgaa
     3481 agtataactt cttctatctc gcccgcatgc acggggtcaa ggttgggatc ttcgcgttct
     3541 ttgagcgcct tggcgagggc acggtccccg ccaatcacga tctggacggc ggtctcggtt
     3601 tcacccaccg gcaggttctg ctccagaaag gcaatgcg
//
LOCUS       DAOQJF010000338         3621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16159_length_3621_cov_5.293606, whole genome shotgun sequence.
ACCESSION   DAOQJF010000338 DAOQJF010000000
VERSION     DAOQJF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16159_length_3621_cov_5.293606"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLF85_14495"
     CDS             complement(<1..251)
                     /locus_tag="PLF85_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20930.1"
                     /translation="MSKIRNLLGGFSAALVALPQAIAFGVTVYVAIDPSLSGAGAIAG
                     ILGVIVLGIVNGSVGRNPILISTPSAAATAYLAAMVLQL"
     gene            350..1348
                     /locus_tag="PLF85_14500"
     CDS             350..1348
                     /locus_tag="PLF85_14500"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE20931.1"
                     /translation="MRFKDQKVTVTGAAGFIGRKLVEQLLAEGAIITAVDLPGVPFPA
                     WSGVECVNSDLSDAAQLKQLCSQKDFIFHLAFPVSDWEKEEKFQQTLKHSEEICRLAA
                     VAGTRLVITTSVVVYADQIGKGPITEETPYGKANGHYMAYKQKQERIALQYAHEHKAD
                     IRIVRPANVYGAGSKPWVVEVARVLAKGVPGLIDGGKGRAGLVLVDNVVEVLLLTAKA
                     DLPRGEIFLAVDDNTVTWHDYFGAIADFIKADAPKSIPRFLAEIAATGGELTWKTLGL
                     AGRPPLTRQALMLVGADNDFKNDKIKRVLGFKPVKTHEQGLWEIAAFLAKNTWQEK"
     gene            complement(1362..2180)
                     /locus_tag="PLF85_14505"
     CDS             complement(1362..2180)
                     /locus_tag="PLF85_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE20932.1"
                     /translation="MATLLVRPIQSLDAPELEAYRTLRRVEEHEHAGIFVATNVKVVQ
                     RLFASDFEVVSALLTPDWFEKFKPSLESRSEKEIVVYVGEKALLETVTGYHLHQGAMA
                     VGKIPRQQTLDELLACSPRPLLIAAAEGIASTENLGALVRSAAAFGTHFLIVGETCVS
                     PLQRRAVSGSMGTIFQQPFVQTENLVGALQTLKASGVRCFAAHLSADAKKLSTVDLRG
                     DCCIVFGAEGPGLSEAVVSACDEVVQIPMPSHMNSLNVAAATAVFLYEATRQRG"
     gene            2272..3057
                     /locus_tag="PLF85_14510"
     CDS             2272..3057
                     /locus_tag="PLF85_14510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE20933.1"
                     /translation="MPNQRPFALVTGASAGLGKEFALQLAERYNLVLVARRADLLQDL
                     AHQCAGRGAECETMTADLLKAEDVARVEERAAKPDVALLVNNAGFGTYGAFADLPRQG
                     EADQVQLNIHTLVRLTHAALGTMTQRGTGSIINVASVAGYQPAPYSATYSATKTFVRY
                     FTEAIAEEVRPKGVYLQVLCPGFTHTEFHEKADIAKGGIPSFLWMESGEVVAASLAAM
                     DRSETVCIPGGVNKAITAVGGATPNAVSAKLAALVMRVGMGAK"
     gene            3070..>3621
                     /locus_tag="PLF85_14515"
     CDS             3070..>3621
                     /locus_tag="PLF85_14515"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE20934.1"
                     /translation="MPTLQWLTNDTEALAEAERSRKPIVLQFIRENCAGCSKMEHHVF
                     SDPEIQRLLGTDFVLLRQNILTDRAVRSRYAALWTPSFYFLNTDGKLLWFSEGPLDAA
                     DFRTVCALGRSRYLLHKGRYEEVIDLIDARFAARENDPRGPQLIFTRGMAYYLRFGDK
                     DAFHAAMEEIVERYPHSPEARMWP"
BASE COUNT          784 a         1093 c         1034 g          710 t
ORIGIN      
        1 ttgagctgca gaaccatagc ggcgaggtat gccgtcgccg cagccgaggg cgtgctgatc
       61 agaatggggt tgcgcccgac agaaccattg acgataccca gcacgatgac gccgagaata
      121 cccgcgatgg caccggcgcc cgacagagac gggtctatcg cgacatagac agtgacacca
      181 aaggctatcg cctgcggcag agccacgagt gccgctgaga agccgccgag cagatttctg
      241 attttgctca ctgcgactgt ttgaagtatt ttcacattgg gtaaaccgat aactctggcc
      301 cggcgacgac tttgattgcc cgccgtgctg gcattcgcaa atcaacctta tgcgatttaa
      361 agaccaaaag gtcaccgtca ccggcgcggc gggtttcatt ggccgcaagc tcgttgaaca
      421 attgctggct gaaggggcaa taatcaccgc agtcgatctg ccgggtgtgc cgttcccggc
      481 gtggagcggc gttgaatgcg taaactctga cctatctgat gccgcacagc tcaaacagct
      541 gtgctcgcag aaggatttta tctttcatct cgcttttccc gtctcggact gggaaaaaga
      601 agaaaagttt cagcagacgc taaagcacag cgaagagatc tgtcgccttg ccgccgtcgc
      661 gggcacgcgg ctcgtgatta ccacatcagt ggtcgtgtac gccgaccaga tcggcaaagg
      721 ccccattacc gaagaaactc cttatggtaa agccaatggc cactacatgg catacaaaca
      781 gaaacaagaa cgcatcgcgc tgcagtacgc gcacgaacac aaggccgaca ttcgcatcgt
      841 gcgcccggca aacgtctacg gtgccggttc gaaaccgtgg gtcgtcgaag tggcgcgcgt
      901 gcttgcgaag ggtgtgccgg gcctcatcga cggcggtaag ggccgcgcag gtcttgtact
      961 cgttgacaac gtcgtcgagg tgctgctgct cacagcaaag gcggatttac cccgcggcga
     1021 aatctttctc gcggttgatg ataacaccgt aacctggcac gactattttg gcgcgattgc
     1081 cgacttcatc aaagctgacg ctcccaaaag tataccgcgc tttctggctg agatcgctgc
     1141 gaccggcgga gagctgacct ggaaaacgct cggccttgct ggtcgcccgc cgctcacgcg
     1201 gcaggcgctg atgctcgtcg gcgccgataa cgatttcaag aacgacaaga tcaagcgcgt
     1261 gctcggcttc aagccggtga agacgcacga gcagggcctg tgggagatcg cggcttttct
     1321 ggcgaaaaat acctggcagg aaaagtaacc ccgcgaagag attaaccgcg ctgtcgggta
     1381 gcctcgtaga gaaacaccgc tgtcgctgcg gcgacattga gcgagttcat atgactcggc
     1441 atcggaatct gcaccacttc gtcgcaggct gagaccacag cctcgcttag gcccggccct
     1501 tcggcgccga aaacaataca acagtcacca cgcagatcaa cggtagaaag ttttttcgca
     1561 tcggcactga ggtgagcagc aaagcagcgc acaccgcttg ccttcagtgt ttgcagtgct
     1621 ccgacgagat tctcggtttg aacgaacggt tgctgaaaaa tcgtgcccat cgagcccgag
     1681 accgcacgcc gctgcagcgg gctgacgcag gtttccccga cgatcaagaa gtgcgtacca
     1741 aacgcggcgg cactgcgcac gagcgcgccg agattttcgg tactcgcaat accttcagct
     1801 gccgcgatca gcagcggccg gggtgaacaa gccaataatt cgtcgagtgt ttgctgccgc
     1861 ggaatcttgc cgaccgccat ggcgccctgg tgcaggtgat aacccgttac ggtctcaagc
     1921 agcgcctttt ctccaacgta gaccacgatc tctttttcgc tccgcgactc gagcgagggt
     1981 ttgaatttct cgaaccagtc aggcgttagc agcgccgaga caacttcgaa gtcgctggca
     2041 aacaagcgct gcacaacctt cacgttcgtc gcgacaaaaa tgcccgcgtg ttcgtgctct
     2101 tcgacacggc gcagcgtacg gtacgcttcg agctcggggg cgtcgagcga ttggatgggc
     2161 ctcacaagca gcgtcgccat gtgccgttga actggcaagc ttcgcgtcag gccgtcaacg
     2221 cggttcaaag tcgctttacg ccgagttcag tctgcacggt ttcttcggct tatgccaaac
     2281 caacgcccct tcgctcttgt caccggtgcc tctgccggcc tcggtaaaga attcgcgctg
     2341 caactcgccg aacggtataa cctcgtactc gtcgcgcggc gagctgacct gctgcaagac
     2401 ctcgcgcacc agtgcgccgg tcgcggagcc gaatgcgaaa cgatgacggc cgacctgctc
     2461 aaggctgaag acgtggcgcg cgttgaagag cgcgcggcaa aacccgatgt tgctttgctg
     2521 gtcaataatg caggttttgg tacctacggc gctttcgccg acctgccgcg ccagggcgaa
     2581 gccgatcagg tgcagctgaa tatccacacg ctcgttcgcc tgacccatgc cgcgctcggc
     2641 accatgacgc agcgcggtac aggcagtatc atcaacgttg cctccgtcgc cggctaccaa
     2701 cccgcaccct actcggcgac gtattcggcc accaaaactt tcgtgcgcta ttttaccgaa
     2761 gcgatcgccg aagaagtccg gcccaagggt gtctacctgc aggtactctg ccccggcttc
     2821 actcacaccg aattccatga gaaggctgat attgccaaag ggggaattcc ttctttcctg
     2881 tggatggagt cgggcgaagt agtcgctgca tcgctcgcag ctatggatcg aagtgaaaca
     2941 gtttgtattc ccgggggcgt caacaaggca atcaccgccg tcggcggtgc aacgccgaac
     3001 gccgtgagcg cgaaacttgc tgcgctggtc atgcgtgtcg gcatgggggc aaaatagcag
     3061 gccagaacca tgcccacatt gcagtggctt actaacgaca cagaagcact cgctgaagcc
     3121 gaacgctcgc gaaaacccat agtgctgcag ttcatccgcg agaactgcgc cgggtgcagc
     3181 aagatggaac accatgtctt ttcagaccca gaaattcaac gcctgctggg caccgatttt
     3241 gtcctgctga gacagaacat cctgaccgac cgcgcggtgc gctctcgcta cgcagcgctc
     3301 tggactcctt ctttctattt cctgaatact gacggcaaac tgctgtggtt ttccgagggc
     3361 ccgctcgacg ctgcggattt cagaacggtc tgcgccctgg gtcgttcgcg ctacctgtta
     3421 cacaagggcc gttacgaaga agtcatcgat ctcatcgatg cccgctttgc agcgcgcgag
     3481 aatgacccgc gcggcccgca gctgattttt acccgcggca tggcgtacta cctgcgcttc
     3541 ggcgacaagg acgcattcca cgccgcgatg gaagagattg tcgagcgcta cccgcacagc
     3601 cccgaggcgc gcatgtggcc c
//
LOCUS       DAOQJF010000339         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16270_length_3609_cov_4.437535, whole genome shotgun sequence.
ACCESSION   DAOQJF010000339 DAOQJF010000000
VERSION     DAOQJF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16270_length_3609_cov_4.437535"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PLF85_14520"
     CDS             <1..1202
                     /locus_tag="PLF85_14520"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20935.1"
                     /translation="DENVVKRGSKYLGEQLATASTRDLKDGYALNRLMFAQMVHLQLA
                     KKSPLKEKIRAEWAALIGAGTKDPYALSLMLQGATAAAWADTRAGAVNLLAGLKKTNQ
                     QGSYFEQEKSRAWYWYSDREEITAQVLYGLQVAGENKKIVQRELISYLIAQKKQRRWR
                     STKLSALVTRAFAAWAATSGETAGKVRVTAEVDGERRDVEYDPRKSSAADLLLSFKTK
                     SRDARVNLSRSSSGFFLARAEWKHYLNKPLIVPREGNFALGRKFFAVDRSGNNYSKGA
                     AKYNFKAGDLVLTEVDLKAEKGAEYLLLEDMIPAGFEPLAPAELAQMGNLRYDGYDNA
                     PAAVTRLDDRVGLAKTWMGKDHFVPRAFYRAVFPGKYQAMPAQGGLMYYPESFAYSAS
                     DVITISE"
     gene            1199..1720
                     /locus_tag="PLF85_14525"
     CDS             1199..1720
                     /locus_tag="PLF85_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20936.1"
                     /translation="MIAASGRLFIAVVLFALLLWPALSAPPTYELLIAAPNAAPADRV
                     AGLLERNLPEVALSGILPAERYRKYARFPVAACSGSEYYLLAHGKRLIAFGCIDDASA
                     ATELQKIRRALARDRDTVRESTTEALYDGALNSAAAGELPESRRYAGLARRRPQADTR
                     LVDRLQRVLDRAR"
     gene            1770..>3609
                     /locus_tag="PLF85_14530"
     CDS             1770..>3609
                     /locus_tag="PLF85_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE20937.1"
                     /translation="MIRVKLSHITDYTYDRPVRLSPHVVRLRPAPHTRTIIESYSLSV
                     DPARQFLNWQQDPFANWQARLVFPEPTRQLSVRVDLVAQIRVFNPFDFFTEEYAAKYP
                     FEYAPRLKHELLPYLAVTGDSPRLRAFAADFTEKDLTIVDFIVALNQYVRNHVGYVIR
                     LEPGVQSPHETLEKKSGSCRDSAYLLVQLARCLGLAARFVSGYLIQLKADKPGEGELK
                     GAATDFTDLHAWAEIFIPGAGWVGLDATSGLLAAEGHIPLAATPEPENAAAIDGFTDP
                     CESELAFSMKVQRLTESARVTRPYNDAVWASVEKLAAKIDADLEKNDVRLSIGGEPTF
                     VSATDRESRQWNYDALGADKYRHAELLLARFYEIYAKGGIVLRSQGKWYPGEILPRWS
                     LDCFWLRDGKPLWHSPGLLLHNGEAAGAVLGDAERFITDVAGELGLPAECIHTAFEDP
                     VYYLYAEAKLPPKFEKIIKGNSYPEIERRRLLRVLDTGLEKPVGFALPLTYNATGQHW
                     ESAELEFRRGFMSLLPGDSPMGLRLPLDSITDAWQQDYHHDPVANGRGDVRKAPSAGR
                     IRRDPIRTFLCAEVRGGALHVFLPPVNTLEEFCGLLSVIEAVAKRRE"
BASE COUNT          779 a          998 c         1079 g          753 t
ORIGIN      
        1 tggatgaaaa cgtcgtgaag cgtggcagca aatacctcgg cgagcaactg gcgacagcga
       61 gtacgcgcga tctgaaagat ggttatgcgc tgaatcgcct gatgtttgcg cagatggtgc
      121 acctccagct ggccaaaaag tcacctttaa aagaaaagat ccgtgcagaa tgggcagcgc
      181 ttataggtgc gggaacaaaa gatccgtacg cgctttcgct catgctgcag ggcgcgaccg
      241 ctgcggcatg ggcagatacg agggccggcg ctgtgaacct gctcgcaggc ctgaagaaaa
      301 ccaaccagca gggcagctat ttcgagcagg aaaaatcgcg cgcgtggtac tggtacagcg
      361 accgtgaaga aattaccgcg caggtgctct atggcctgca ggtggcgggt gagaacaaga
      421 agattgtaca gcgcgagctg atcagttatc tcattgcaca gaagaagcag cggcgctggc
      481 gcagcaccaa actctcggcc ctggtgacgc gcgcttttgc cgcctgggca gcgaccagtg
      541 gcgagactgc tggcaaggtg cgagtgactg cagaagtcga cggtgaacgc cgcgacgtcg
      601 aatatgaccc gcgcaagagt agtgcggcag acctgctgtt atcattcaaa accaaatcgc
      661 gcgatgcgcg cgtaaaccta agccgcagca gttcaggctt ctttctggcg cgcgccgagt
      721 ggaagcacta cctcaacaaa ccgctgatcg tgccgcgcga aggcaatttc gcgctcggcc
      781 gcaaattttt cgccgtcgac cgcagcggca ataactacag taaaggtgca gcgaagtata
      841 acttcaaagc gggcgatctg gtcttgaccg aggttgacct gaaagcagag aagggtgcgg
      901 agtacctgct gctcgaagac atgatacctg caggatttga accgcttgcc ccggccgagc
      961 tggcgcagat gggtaatttg cgctacgacg gttacgacaa tgcaccggct gcggtaaccc
     1021 gcctcgacga ccgagtgggg ctggctaaga cctggatggg caaagatcac tttgtaccac
     1081 gcgcgttcta ccgcgccgtt ttcccgggca aataccaggc aatgcccgcg cagggtggac
     1141 tcatgtacta tcctgaatct ttcgcgtatt cggcgtcaga cgtgatcacg attagcgaat
     1201 gatcgcagcc agcggcaggt tgtttatcgc ggtggtgctt tttgcgctgc tgctgtggcc
     1261 ggcattgtct gcgccgccta cctatgaact gctcatcgct gcaccgaatg ctgcgccggc
     1321 agaccgggtc gccggtttgc tcgagcgtaa cctgcctgaa gttgcgttaa gcggaattct
     1381 gcctgcggag cgctatcgca aatacgcacg atttccggtc gctgcctgca gcgggagcga
     1441 atactatctt ttggcgcacg ggaagcggct tatcgccttc ggctgtattg acgatgcctc
     1501 cgcagcaaca gaactgcaga aaatccgccg cgcgctggca cgtgatcgcg acacggtgcg
     1561 cgaaagcaca accgaagccc tctatgacgg ggcactcaac agtgcggcag caggcgaact
     1621 gcctgaatcc agacgttatg ctggcttggc tcgcaggcga ccacaagccg atacacgcct
     1681 tgtcgatcgc ctgcagcgcg tgttagaccg ggcgcggtag atttttgagc caccggatct
     1741 tggcatgaga tttgcagcat cctggctgcg tgatccgggt aaaactcagc cacattaccg
     1801 attataccta cgatcgcccg gtcaggctgt cgccgcacgt cgtgcgcctg cgtccggcgc
     1861 cgcatacgcg tacaatcatc gaatcgtatt cgctcagtgt tgatccggcg cggcagtttc
     1921 ttaactggca gcaggaccca tttgccaact ggcaggcacg gctggtattc cctgagccga
     1981 cgcggcagct cagcgtcagg gtagatctcg tggcgcagat cagagtgttt aatccctttg
     2041 attttttcac cgaagaatac gcggctaagt acccgttcga atacgcgccg cggctgaaac
     2101 atgaactttt accctacctt gcagtgaccg gcgatagtcc gcggttgcgt gcatttgcgg
     2161 ctgactttac agagaaagat ctcacgattg tcgactttat cgttgccctg aaccagtacg
     2221 tacgcaatca tgtgggttat gtgattcggc tcgaaccggg cgtgcagagc ccgcacgaaa
     2281 cgctcgaaaa aaaatcgggg tcgtgccgtg acagtgccta cctgctggtt caactggcgc
     2341 gctgtctcgg tcttgccgca cgtttcgttt ctggttacct gatacagctc aaagccgaca
     2401 agccgggcga gggggagctt aagggtgcag ctaccgactt caccgacctg catgcatggg
     2461 ccgagatttt tataccgggt gcaggctggg tgggactcga tgccacttct ggtctgcttg
     2521 cggccgaggg acatataccg ctggcggcaa ctccagagcc cgagaatgcc gcagcaatcg
     2581 acggttttac cgatccctgt gaatcagaac tggctttcag tatgaaagtc caaaggctga
     2641 ctgaatcagc cagggttacc cgtccttaca acgatgcagt gtgggcctcg gttgaaaagc
     2701 ttgcggcgaa gattgatgct gacctggaaa aaaacgatgt gcgcctgtcg atcgggggcg
     2761 agcccacatt tgtttcggcg accgaccgcg agagccggca atggaattac gatgcgcttg
     2821 gcgccgacaa ataccgccac gcagagctgt tgctcgcccg cttctacgaa atctacgcca
     2881 agggcggtat tgtgctgcgt tcgcagggca aatggtatcc cggcgaaata ttgccgcgct
     2941 ggtcgctcga ctgcttctgg ctgcgcgacg gcaagccatt gtggcatagt ccgggtttac
     3001 tgctgcacaa tggcgaggcg gctggtgcag tcctcggcga cgccgagcgc ttcattaccg
     3061 atgtcgccgg tgagctgggt ttaccagccg aatgtataca tacggctttt gaagaccctg
     3121 tctattacct ctacgccgag gcgaaactgc cgcccaaatt cgaaaagatt atcaagggca
     3181 attcgtatcc tgaaattgaa aggcgaaggc tgctgcgcgt tctcgatacc gggcttgaaa
     3241 agccggtagg ttttgcgctg cccctgactt acaatgcaac cgggcagcac tgggaaagtg
     3301 cagaactaga gttccggcgg ggatttatgt ctttgctgcc cggtgattca cccatggggt
     3361 tgcggctgcc gctcgattcg attaccgatg catggcagca ggactaccac catgacccgg
     3421 tggccaatgg ccgcggtgat gtgcgcaagg cccccagcgc cgggcgtatt cggcgcgatc
     3481 cgatacggac ttttctctgt gcagaagtgc gcggcggtgc gctgcatgtc tttttgcccc
     3541 cggtaaatac cctcgaagaa ttctgcggat tactttcggt tatcgaagcc gtagcgaaac
     3601 ggagagaac
//
LOCUS       DAOQJF010000340         3608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16282_length_3608_cov_3.257247, whole genome shotgun sequence.
ACCESSION   DAOQJF010000340 DAOQJF010000000
VERSION     DAOQJF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16282_length_3608_cov_3.257247"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..2641
                     /gene="acnD"
                     /locus_tag="PLF85_14535"
     CDS             23..2641
                     /gene="acnD"
                     /locus_tag="PLF85_14535"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HNE20938.1"
                     /translation="MQSQMNTKYRKKLPGTNLDYFDAREAVNAIEAGAWDKLSYTARL
                     FAENLVRKAEPENLKDYLGQLIYNKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAI
                     AQKGGDPSKINPVVQTQLIVDHSLAVEYGGYDKDAFRKNREVEDRRNDDRFHFINWTK
                     EAFENVDVIPPGNGIMHQINLEKMSPVVHAQDGVAFPDTMVGTDSHTPHVDALGVLAV
                     GVGGLEAESVMLGHPSFLRLPDIIGVELTGKPQPGITATDLVLYLTEFLRKEKVVSAH
                     VEFYGEGVQHLTLGDRATISNMTPEYGATAAMFFIDQQTIDYLKLTGRTDENVKLVEV
                     YAKEAGFWADSLKNVKYERVLKFDLSSVQRTMAGPSNPHKRVSTTDLAKNGIAGAWTE
                     EAGKMPDGAVIIAAITSCTNTSNPRNVISAGLLARNANAKGLVRRPWVKSSLAPGSKV
                     VQSYLEEAKLLPELEKLGFGVVAFACTTCNGMSGALDPKIQQEIIDRDLYATAVLSGN
                     RNFDGRIHPYAKQAFLASPALVVAYAIAGTVRVDIEKDPLGKDAQGNPVYLKDIWPSD
                     AEIDKIVAQSVKPEQFRAVYDPMFARGAASTKKISPLYDWRPQSTYIRRPPYWDTEGV
                     GALAANPRTLKNLRPLAILPDNITTDHLSPSNAILRNSAAGEYLEKMGLPEEDFNSYA
                     THRGDHLTALRATFANPQLLNEMCLDDKGQVKKGSLARVEPEGKVMRMWEAIETYLNR
                     KQPLIIVAGADYGQGSSRDWAAKGVRLAGVEVIAAEGFERIHRTNLVGMGVLPLEFKP
                     GTTRKTLNLDGTEVYSVEGKIAPRAELTLVIERKSGEVVKVPMTCRLDTAADVSVYEA
                     GGILQRFANNFLASKN"
     gene            2868..>3608
                     /locus_tag="PLF85_14540"
     CDS             2868..>3608
                     /locus_tag="PLF85_14540"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE20939.1"
                     /translation="MNDPAMKPADYSRALTYLCKAMGKFYMTNFEEDQKKYCIEVAEK
                     LRAKGAQINTVNKQETALLSSIGQLQEVYFDYLMKNGADPNLANTDGETPLMKAVYKE
                     PYAQRSFTKKTNERYFTESLLKAGAKINAKSKNGRTALHVVAYWGHSQAADFLIKKGA
                     ALEVKDKDGLTPMQVAQIENRNAVVSSLKSAGAKETPLPAKYLAARQKNAENQKMAQA
                     EAEIAQAKENARKKSEANKAYEEQLKGLK"
BASE COUNT          901 a         1054 c          969 g          684 t
ORIGIN      
        1 aaagtccttg attttggctc cgatgcaatc ccaaatgaac accaaatacc gtaaaaaact
       61 cccggggact aacctcgatt atttcgacgc ccgcgaagct gtgaatgcaa tcgaagccgg
      121 cgcgtgggac aagctctctt acaccgctcg cctgttcgcc gaaaatttag tgcgcaaagc
      181 cgagcccgaa aatctcaaag actacctggg tcagctcatt tataataagc gcgacctcga
      241 cttcccgtgg tacccagcac gcgtcgtctg ccatgacatt ttaggccaaa ccgcgctcgt
      301 cgacctcgcc ggcctgcgcg acgcgatcgc gcaaaaaggc ggagatccct caaagatcaa
      361 cccggttgtc cagacgcagc tcatcgtcga ccactcgctc gcggtcgaat acggtggtta
      421 cgacaaagac gcctttcgca agaaccgcga agttgaagac cgccgcaacg acgaccgctt
      481 ccacttcatc aactggacca aagaagcgtt tgaaaacgtc gacgtgatcc cgcccggtaa
      541 cggcatcatg caccagatca acctcgagaa gatgtcgccg gtcgtgcatg cgcaagacgg
      601 cgtcgctttc cctgacacca tggtcggtac cgactcacac accccgcacg tcgacgcgtt
      661 gggagtactc gcggtgggtg tcggtggcct cgaagcggaa agcgtcatgc tcggccatcc
      721 gtcatttctg cgtctgcccg acatcatcgg tgttgagctg accggtaaac cacagccagg
      781 catcactgcc accgacctcg tgctctacct gacagaattt ttgcgcaaag agaaagttgt
      841 ctcagctcat gtggagttct atggtgaagg cgtgcagcac ctgacgctgg gtgaccgcgc
      901 gacgatctcg aacatgactc ctgaatacgg ggcaactgcc gcgatgttct tcatcgacca
      961 gcagactatc gactatctca aactcaccgg ccgcaccgac gagaatgtta agctcgtcga
     1021 agtttacgcg aaagaagccg gcttctgggc cgacagcctc aagaatgtaa aatacgagcg
     1081 tgtgttgaaa tttgacctgt catcggttca gcgcacgatg gccgggcctt cgaacccgca
     1141 caagcgcgtg agcacgaccg acctcgcgaa aaacggcatt gccggcgcgt ggactgaaga
     1201 agcgggcaag atgcccgacg gcgcggtcat catcgcagcg atcaccagct gcaccaacac
     1261 ctctaacccg cgcaacgtga tctctgccgg ccttttggcg cgtaacgcga atgcgaaagg
     1321 cctcgtgcgc cgcccgtggg taaagtcatc cctcgccccg ggatctaaag tagtacagtc
     1381 atacctcgaa gaagcaaagc ttctgcctga actcgaaaaa ctcggcttcg gtgtggtggc
     1441 attcgcttgc acaacctgca acggtatgtc tggcgcgctg gacccgaaaa tacagcaaga
     1501 gatcatcgac cgtgacctct acgctacggc cgtgctttct ggtaaccgta acttcgacgg
     1561 gcgtattcac ccgtacgcca agcaggcatt cctcgcttcg ccggcgctcg tcgtcgcata
     1621 tgcgatcgcg ggcacagtgc gcgtcgacat cgaaaaagat cccctcggca aagacgcaca
     1681 gggcaacccg gtgtacctca aggacatctg gccatcggat gcagagatcg acaagatcgt
     1741 tgcgcagagc gtgaagcccg agcagttccg cgctgtctat gacccgatgt ttgcgcgcgg
     1801 tgccgcctcg acgaagaaga tcagtcccct ctacgactgg cgcccacaat cgacctatat
     1861 ccgccgccca ccgtactggg ataccgaagg cgtcggtgca cttgccgcga acccacgcac
     1921 gctcaagaac ctgcgcccgc tggctatctt gcccgacaac attaccactg accacctttc
     1981 gccttcgaac gcgattttgc gcaacagcgc ggccggtgag tacctcgaga agatgggcct
     2041 gcccgaagaa gattttaact cctacgcaac gcaccgcggc gaccatttga ccgcgctgcg
     2101 tgcaaccttt gcgaacccgc agctcttgaa cgagatgtgc ctcgacgaca aaggccaggt
     2161 gaagaaaggt tcactcgcgc gcgttgagcc cgaaggcaaa gtcatgcgca tgtgggaggc
     2221 tatcgaaact tacctcaacc gcaagcagcc gctaatcatc gttgccggtg ccgactacgg
     2281 ccagggctcg tcacgcgact gggcggcgaa gggtgtacgc ctcgcgggcg tcgaagtcat
     2341 cgcggccgaa ggttttgagc gtatccaccg cacaaacctc gtcggtatgg gtgtgctgcc
     2401 gctcgaattc aaaccgggca caacacgcaa aacgctgaac ctcgatggca ccgaggtata
     2461 ctcggtcgaa ggtaagattg caccgcgcgc cgagctcact ctcgtgatcg agcgcaagag
     2521 tggcgaagtg gtgaaggtgc caatgacctg ccgcctcgac accgcggccg acgtatcggt
     2581 ctacgaagcg ggcggtattc tgcagcgctt cgcgaataat tttctcgcgt cgaagaattg
     2641 aaatcaccag gcagggaata aattccctgc ctggtgaatg ccaaatgcat tgacgggccg
     2701 ctcaggccaa atcttttgcg cgcatgaatc ggattgcacg cccgctactg gctctctgcg
     2761 cctgctcatc tgttatcttc cccgagtttg ataaggctga attcgagttc agctcaggat
     2821 ttcttgatcc ccgcatttac ggtgggatgt caaaggtcga aaaggtgttg aatgatcccg
     2881 cgatgaagcc tgctgactac tccagggctt tgacctacct atgcaaagcc atgggcaaat
     2941 tttacatgac caactttgag gaagatcaga aaaaatactg cattgaagtc gccgaaaaac
     3001 tgcgcgccaa aggagcgcaa ataaataccg tgaacaagca ggaaaccgcc ttgctttcca
     3061 gtattggcca attgcaagag gtatatttcg attacctgat gaagaacggg gctgatccga
     3121 accttgcgaa tactgatggc gagacgcctc tcatgaaagc ggtctacaaa gagccatacg
     3181 cgcagcggag cttcacgaaa aagactaatg aacgctattt tactgaaagt ctattgaaag
     3241 ccggcgctaa gatcaacgct aaaagcaaga acggaagaac ggcgttacat gtggtcgcct
     3301 actggggtca ttctcaggcc gctgattttc tgatcaagaa gggggccgca ttagaagtga
     3361 aggacaagga cggcctcacc cccatgcagg tcgcgcaaat tgagaatagg aacgctgtgg
     3421 ttagctccct caagagtgcg ggcgccaaag aaactcccct gccggcaaaa taccttgctg
     3481 cgcggcagaa gaatgcagag aaccaaaaaa tggcacaagc cgaggcagaa atcgcccaag
     3541 cgaaagagaa cgcgcgaaag aaatctgagg caaataaggc ttatgaagag cagctaaagg
     3601 gcctcaaa
//
LOCUS       DAOQJF010000341         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16522_length_3576_cov_6.353593, whole genome shotgun sequence.
ACCESSION   DAOQJF010000341 DAOQJF010000000
VERSION     DAOQJF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16522_length_3576_cov_6.353593"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLF85_14545"
     CDS             <1..305
                     /locus_tag="PLF85_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20940.1"
                     /translation="GLEGVNNIFAVTFVQEYCAGKGNTIFFFWNGKKLLHAHSSIDGA
                     DAPHYATEKQIFPADKAGRKGYVILEQEYGDHDDPQSVKREKILLKWNGKKLEKTS"
     gene            302..658
                     /locus_tag="PLF85_14550"
     CDS             302..658
                     /locus_tag="PLF85_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20941.1"
                     /translation="MKKMTLAILVAALSVATTYAVKKQRLHAAEVTYESIRGHTESYG
                     SSEKHVVVVLLNGGEKDTVVSGSVIAKNSAKGYTVANCPFKTGVPAGSEVKKKIKCQV
                     ADADSLELVITPGAVK"
     gene            694..1113
                     /locus_tag="PLF85_14555"
     CDS             694..1113
                     /locus_tag="PLF85_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20942.1"
                     /translation="MVAVVAAVALVGFAGAALASVLAPKISLSIGLFFFVIGVWWANT
                     PSKFAILRNFVFIYASSLIMMGCIVALGFGIMGLTPLNYWVSQYQVDRQSLALSATTG
                     LLFLWGTGEAVARGLKKVALKKTPTRNWFEALVETIF"
     gene            1116..2057
                     /locus_tag="PLF85_14560"
     CDS             1116..2057
                     /locus_tag="PLF85_14560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE20943.1"
                     /translation="MQDLANRLARMKKHLGKWARRRGITCYRLYERDIPEYPFIADLY
                     EDHMILTELASEAVQSRPDYAAWKAGIVELFRSTWEIPHDKLHLKTRFRRAEGEQYEK
                     LAEGDSVVVHESGLKFFIYPENWLDTGLFLDHRNLRARVRELAQGKAMLNLFCYTGSF
                     SVYAAAGGASLVHSVDLSAKYLGILEENLKLNGLQNTPHENIRLDSREYLQQTPAQFY
                     DIIVCDPPVFSRSRKQERDFDVLRDHPQLIRDCLRVLKPGGVLFFSTNFRKFRIRFPE
                     EGDGYQKTQVKNITAQTIPEDFRNKWIHACYEIRHGA"
     gene            2176..2388
                     /locus_tag="PLF85_14565"
     CDS             2176..2388
                     /locus_tag="PLF85_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20944.1"
                     /translation="MNALRTTYDDLPETVTLNIPKEMAHRRGEIIVIVEETPVDNRKE
                     LLDFFGSIPDFPDRGDQGEPDNRESL"
     gene            2385..2786
                     /locus_tag="PLF85_14570"
     CDS             2385..2786
                     /locus_tag="PLF85_14570"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE20945.1"
                     /translation="MKYLLDTNVCIQVLRGSLPQIKSRIAAIRHDQIGIPAIVRFELV
                     YGAWKSSNPEQKVALVQNFTSAFASVPVTNDVADSCGKIRAMLEKLGKPIGPYDLLIA
                     ATALANGLVLVTHNTREFMRIPGIQLEDWEQ"
     gene            complement(2856..3356)
                     /gene="tpx"
                     /locus_tag="PLF85_14575"
     CDS             complement(2856..3356)
                     /gene="tpx"
                     /locus_tag="PLF85_14575"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNE20946.1"
                     /translation="MAKVTLRGNALTTIGSLPAVGSKVPDFRLTKQDLSELKPADLAG
                     KKVIYNIFPSLDTATCATSVRKFNEKAAGLDNTVVVCVSSDLPFAQKRFCGAEGIANV
                     VTGSDVRDKEFGKRWGLVFTDGPLEGLMSRSIVVADGEGKVIYTEQVGETANEPDYDK
                     ALAAAK"
BASE COUNT          851 a          932 c          965 g          828 t
ORIGIN      
        1 gtggtctcga aggggtgaat aacatctttg ccgtaacgtt tgtccaggag tattgcgccg
       61 gcaaaggaaa cacgatattt ttcttctgga acggcaagaa actgctgcat gcccacagct
      121 cgattgacgg tgccgacgca ccgcactacg cgacagaaaa gcaaatattt cccgcagaca
      181 aagctggccg caagggttac gttattctcg agcaggaata cggcgaccat gacgatccgc
      241 aatccgtaaa aagagaaaaa atcctcttaa aatggaatgg caaaaaactg gagaagacat
      301 catgaaaaaa atgaccctgg caatcctggt cgctgccctg tcggtagcaa ctacctacgc
      361 ggtaaaaaag cagcgtctgc atgcagctga agttacctat gagagcatac gcggccatac
      421 agaatcgtac ggttcttcag aaaagcacgt ggtggttgta ctgctgaacg gtggcgaaaa
      481 agatacagta gtttcgggtt cagtcattgc gaagaacagc gccaagggct acacggttgc
      541 caactgcccg ttcaaaaccg gtgtgccggc gggctctgag gtgaaaaaaa agatcaaatg
      601 ccaggttgca gatgctgatt cactggaact cgtcattacc cccggggcag tgaaatagcc
      661 gggtcaatat tttcgtatgc cgtacacttc gccatggtgg cggttgttgc tgccgtggcc
      721 ttggtgggtt ttgcgggcgc tgcactggca tcggtactgg cgccaaagat ttcgctttcc
      781 ataggcttat tcttttttgt catcggtgtc tggtgggcaa acacaccgtc gaagttcgcg
      841 atcctgcgca attttgtttt catttacgca agctctttga tcatgatggg ctgcatagtg
      901 gcactcggtt ttggcatcat gggccttacc ccgttgaatt attgggtaag ccagtatcag
      961 gtcgacaggc aatccctggc gctcagcgca acaaccggac tactgttcct gtggggtacg
     1021 ggtgaggccg tcgcgcgggg cttgaaaaaa gtggcgctca agaagacccc aacccgtaac
     1081 tggttcgaag ccctggtcga aacaatcttc tgaatatgca ggatctcgct aaccgcctcg
     1141 cgcgcatgaa aaagcacctc ggcaagtggg cgcgccgccg cggcatcact tgctaccggc
     1201 tatacgagcg cgatattccg gaatatccgt tcatcgccga cctttatgaa gaccacatga
     1261 ttctgaccga gctggcaagc gaggcagtac aaagccggcc cgattacgcc gcgtggaaag
     1321 ccggcatcgt cgaacttttt cgcagcactt gggaaatccc ccacgacaaa ttgcatctca
     1381 agactcgctt tcgccgcgcc gagggtgagc agtacgaaaa acttgccgaa ggcgacagtg
     1441 ttgtcgtcca tgagagcggc ctgaagttct tcatctaccc ggagaactgg ctcgacacgg
     1501 ggctttttct cgaccaccgc aacctgcgcg cgcgtgtgcg tgaactggct cagggcaagg
     1561 cgatgctcaa cctcttctgc tacacgggct cgttcagcgt ctatgctgcg gcaggcggag
     1621 cttcgctggt gcacagcgtt gatctctctg cgaagtacct tggcatactc gaagagaatc
     1681 tgaaactcaa cgggctgcaa aacacaccgc atgaaaatat ccggctcgac tcgcgcgaat
     1741 acctgcagca gacacccgcg cagttctacg atattattgt ctgcgacccg ccggtatttt
     1801 cacgcagccg caaacaggaa cgcgactttg acgtgctgcg tgaccacccg cagctgattc
     1861 gtgactgcct gcgcgtgctc aagccagggg gagtgctatt tttttcgact aacttccgca
     1921 aattccgcat ccgttttccc gaagagggag atggctacca aaagacgcag gtgaagaaca
     1981 tcacggcgca gacgattcct gaagacttca ggaacaagtg gatccacgcg tgttatgaga
     2041 ttcgccatgg cgcgtgaggc tatatcgtgt ctggtagcca ataatcctta ctgatagaat
     2101 ctcaagcagc ctggcaaatt tctgctgttc cttgacagct cgttacccca ccggcaagtt
     2161 gatgttcggt tacagatgaa tgcattgcgc acaacatacg acgatctgcc ggaaacagtg
     2221 accctcaata tcccgaaaga gatggctcac cggcgcggtg aaattatcgt cattgtcgaa
     2281 gaaaccccgg ttgataaccg caaagaactt ctggatttct ttgggtccat tccggacttt
     2341 cctgatcgcg gtgaccaggg agaaccggat aaccgcgaat ccctttgaag tatcttctgg
     2401 acaccaatgt ttgtatacag gttctgcgtg gctcactgcc gcagataaaa agccgtattg
     2461 ctgcaattcg ccatgaccaa atcggcatcc cggcaattgt acgttttgag cttgtctatg
     2521 gtgcatggaa aagcagcaac ccggagcaaa aggtagcttt agttcaaaat tttacgagtg
     2581 cgtttgccag tgtgcccgta acaaatgacg tcgctgacag ttgcggtaaa attcgggcta
     2641 tgctggagaa gctgggtaaa ccaatagggc cttatgatct tcttattgca gcaaccgcgt
     2701 tggccaatgg cctggtatta gttacacaca atacacgcga gttcatgcgc attccaggca
     2761 ttcagctcga agattgggaa cagtgatgac agaccttgca gtttaccgtg aagaagcagc
     2821 gcttcagctg gctcttcacg gttgcctttt gtcagctatt tcgccgcggc gagcgccttg
     2881 tcgtaatcag gctcgttggc ggtttcgcct acctgctccg tgtagataac tttgccctcg
     2941 ccatcggcga cgacgatcga acgcgacatg aggccttcga gtgggccatc ggtaaagacc
     3001 aggccccagc gtttgccaaa ctctttgtcg cgcacatcag aaccggtaac aacgttagcg
     3061 ataccttctg caccacagaa gcgcttctgc gcaaagggga ggtcagaaga aacgcagacg
     3121 acaacagtgt tatcgagccc tgcagctttt tcgttgaact tgcgtaccga agtcgcgcag
     3181 gtcgccgtat caaggctcgg gaaaatgttg tagataactt ttttgccagc aaggtctgcg
     3241 ggctttaatt ctgacaggtc ttgttttgtc agacggaaat cgggaacttt gctgccgacg
     3301 gccggtagcg agccaatagt ggtgagggcg ttgcctctga gggttacttt tgccatagtc
     3361 cgttcaatat agcaaatttt tgggttcgga gcgagaattc ggtcaagaag cctgtttact
     3421 gtggctgttt cagcatgcgc gtgaaatagt cgatgccctc ggccacgggg tctgccccgc
     3481 gcgcatggcg tatcatttca ttcaggtcga gcacgatcag cccgtacatc catgcccaca
     3541 tggtgcgcgc gatcgcgggg tagctcttgc gcgggt
//
LOCUS       DAOQJF010000342         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16744_length_3544_cov_5.352537, whole genome shotgun sequence.
ACCESSION   DAOQJF010000342 DAOQJF010000000
VERSION     DAOQJF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16744_length_3544_cov_5.352537"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(891..2045)
                     /locus_tag="PLF85_14580"
     CDS             complement(891..2045)
                     /locus_tag="PLF85_14580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNE20947.1"
                     /translation="MRINLGPSHPATHGILENHLILDGETIEAVDIHIGYVHRCFEKL
                     GEHYGYNQFLVCTDRMNYVSTPMNNIGWILAVESMMGITAPPKALVVRMIISELSRII
                     DHIICLGILGVDLGAFASFLYMFHKREDIYQILEKMTGARLTNTFCRVGGLEKDIYPQ
                     FVADVKKFVKSFPKVLKETDMLLTKSRIFYERTKGVGKISGERALAYGFTGPNLRAAG
                     VDYDVRVSSPYMYYDQVDFDVPVGEDGGVYDRYLVRLEEMRQSLRIIEQLVNNIPEGA
                     YHADIPHAYLPPKEKVYTSMEDLIYHFKIIMHGIEVPTGEHYFSTESANGELGFTIVS
                     RGKKSPWRVHVRRPCFWYYQAFPEIVNGGLLADAIANMSSLNVIAGELDC"
     gene            complement(2247..2705)
                     /locus_tag="PLF85_14585"
     CDS             complement(2247..2705)
                     /locus_tag="PLF85_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20948.1"
                     /translation="MTDKEYEARFMSWVYRYVPWIGAFLVLSQVLGPVVSFTRFQFGT
                     KVDAQFVNRVRLAAGATSTRGIGESSCAYNYAIEGKQHYLIQDCGLLPGFQPEAGRFY
                     PVVFYQQDPPLIVSKVAFAPGWVRLFTCLMGVVLVVLPRVLLPRMKNFPK"
     gene            2878..>3544
                     /locus_tag="PLF85_14590"
     CDS             2878..>3544
                     /locus_tag="PLF85_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20949.1"
                     /translation="MVKVTQLQQVHHILNTVLGKKPCSILVDDAEHPAGFISYTLLTT
                     GPELKIRTQFLNTPAERRVLVRHNKKRIVAVCKFLLRNGPTEILHPHSLEITDDVKKD
                     VAETGNSAGFFMTNLTSAKEIAFVIEANKKKVNGFVLNFFDSELKGLVPYHRIFLATG
                     ESTDMRLRHLVKNPRIIYYTPEPPPDFKPKQYFPQDIYLEEIHKSDLKVPANLKTEVT
                     IPIL"
BASE COUNT          856 a          979 c          955 g          754 t
ORIGIN      
        1 actaccagat catcggcaac aagacgctgc atttttctga ccgtgaaact gcgctgaaat
       61 taatgagcac gttgaagatt cgctgaagtg ggtgattgta gagacgcaat tcattgcgtc
      121 tctacagcta agggcagccc tgaatctgca cggtcgccgt cgcgcctgtg ccgccagtca
      181 ggcagccgac aaggttcacg gtacaaaatg cagtgtaacc agagccggga gtcagcgggg
      241 tgacactgat aatggctccg ccgccaatga aaatattcgc ctgggccaaa gacccgccgc
      301 caccattgca ggggcagcga aatgggcccg gcccattata aaggttgccc ggtgaaaaag
      361 tatagccggt tatctcgtat cccatgacga ccgtgcctga agcgccggtt cccgcgccgc
      421 ccgaaatgct gagcgaaccg gtgaaagccg ggcctgtata accagatcca gcggccgtga
      481 cggtttagct cgcaaccgtt gtgcccgtta acgcggcagt aaaggtcgcg cctgaaccag
      541 ggccggtggc tgtaactgca ggcagcgttg tgaagtttgt accgcctgag gttaccgtaa
      601 cactcgcgat gggtccagtg cgagtgccac tcgattggct ggtcttcttt gtctcgggat
      661 tgccaaagtc tccccagctc tggcaccggg tcaggagcaa taccatcatc agcagggacg
      721 gacgcatcgt gacagtatgg aagacacgaa ccactggcgt caaggcagta ttgatgaatc
      781 gcgagatggc cccgctccac ccggatgggt attcggcgaa ttcgtccagt tcaggtagtc
      841 gtgggggctg tagagacgca attaattgcg tctctacaat cacaggaaat tcagcagtct
      901 aattcaccag cgataacgtt aagcgagctc atgttcgcga tcgcatcggc gagcaggcca
      961 ccgttcacaa tttcgggaaa agcctggtag taccagaagc agggccggcg cacgtggact
     1021 cgccacgggc tttttttgcc tcgtgagacg atcgtgaaac cgagctcccc gttcgccgat
     1081 tcggtcgaga agtagtgctc gccggtcggc acttcaatac cgtgcatgat aatcttgaag
     1141 tggtagatga ggtcttccat cgaggtgtag accttctcct tcggcggcag gtaggcgtgc
     1201 ggaatgtcgg cgtggtaggc gccctcggga atgttattca ccagctgctc gatgatgcgc
     1261 agcgattggc gcatctcttc aaggcgtaca aggtaacgat cgtaaactcc gccatcttca
     1321 cccaccggta cgtcgaagtc aacctggtcg taatacatat agggggagct cacgcggaca
     1381 tcgtagtcaa cacccgcggc gcgcaggttc ggccctgtga aaccatacgc gagtgcacgc
     1441 tcgccggaaa ttttgccgac accttttgtg cgctcataga aaatgcgcga cttggtcagc
     1501 agcatgtcgg tttctttcag tactttcgga aagctcttca cgaacttttt gacatccgcc
     1561 acgaactgtg gatagatatc tttttcgaga ccgccgacac ggcagaacgt attagtcagt
     1621 cgcgcaccgg tcatcttttc gagaatctgg tagatgtctt cgcgcttgtg gaacatgtaa
     1681 agaaacgaag cgaaagctcc caagtcgacg ccgagaatgc ccaagcagat gatgtggtcg
     1741 ataatgcggg agagttctga aatgatcata cgcacgacga gcgccttggg tggcgcggtg
     1801 atgcccatca tcgactcgac tgcaagaatc cagccgatgt tgttcatcgg cgtcgagaca
     1861 tagttcatgc ggtcggtgca gaccaaaaac tggttatagc cgtaatgctc acccagtttt
     1921 tcaaagcagc ggtgcacgta gccaatatgg atgtcgacgg cttcgatggt ttcaccgtcg
     1981 agaatcaggt ggttttcgag aatgccgtgt gtcgccgggt gcgagggccc aaggttaatg
     2041 cgcatgcggc cgtcaggcag ttcgcccacg cgcacaccgg gttgtgtgac aggcaaccag
     2101 cgcgtttctt cgatgggagg aaggttggta aaattgcggt tattggcgtt gacttcgcct
     2161 tgcatagtaa tgcgtccgga gctttagcct accgcgtaaa tccattttcc cgcggcaggc
     2221 tcccgaaagg gaatctggcg taggtattat ttcgggaagt tcttcatccg cggaagtaag
     2281 acccgcggca gcacaaccag aaccaccccc atgagacagg tgaagagcct tacccatccg
     2341 ggggcaaaag cgacttttga gactatcaac ggcggatcct gctggtagaa aaccactggg
     2401 taaaaccgcc ccgcttcggg ctgaaaacct ggcaaaaggc cacaatcctg aatgaggtag
     2461 tgctgtttcc cttcgatcgc ataattgtaa gcacagctcg actctccgat accgcgcgtg
     2521 ctggttgccc ccgcggccag gcgaacccgg ttcacaaact gtgcgtcgac cttagtacca
     2581 aactggaagc gtgtgaagct gaccaccggc cccaaaacct ggctcaggac aaggaacgcg
     2641 ccgatccacg gtacataccg atacacccac gacatgaacc ttgcttcgta ttccttgtcg
     2701 gtcatttctg tacctggcca gcgcacctgc aatgtcaaca ggtttcaatg agagcagtcg
     2761 tgcaaaaaaa aatggcacgc gcttgcctgt gattcaatca attaatatca gctagaaagc
     2821 cgcgccaagt tattgactca ctgtgtctga cctcaacgct gcaaaaaccc ggttctcatg
     2881 gtaaaagtca cccaactaca gcaagtccat catattctga acacagttct cggcaaaaaa
     2941 ccctgctcga tactggtcga cgacgccgaa cacccggcgg gatttatctc ttatacgcta
     3001 ctgaccaccg ggcctgagct aaaaatccgc acgcagtttc tgaatacccc tgccgagcgg
     3061 cgggttctcg tacgacacaa caagaaacgt attgtcgcgg tttgcaaatt tctgctgcgc
     3121 aatggcccga cggaaattct gcacccgcac agcctcgaga ttaccgacga cgtgaagaaa
     3181 gatgtcgcag aaaccggaaa ctccgccggc ttcttcatga ccaacctgac gtcggcaaaa
     3241 gaaatcgcgt tcgttatcga ggccaacaag aagaaggtga acggctttgt gctgaacttc
     3301 ttcgacagtg aactcaaggg cctggtgcct taccaccgca tctttctcgc aaccggcgag
     3361 tcaactgaca tgcggctgcg ccacctcgtc aagaacccgc ggatcatcta ctacacgccg
     3421 gagccgccgc ccgatttcaa gcctaagcag tactttccgc aggatatcta cctcgaagaa
     3481 attcacaaga gtgacctgaa ggtaccggca aatctcaaaa ccgaggtgac gattccgatt
     3541 cttt
//
LOCUS       DAOQJF010000343         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_16875_length_3527_cov_4.752880, whole genome shotgun sequence.
ACCESSION   DAOQJF010000343 DAOQJF010000000
VERSION     DAOQJF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16875_length_3527_cov_4.752880"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="PLF85_14595"
     CDS             complement(<1..1879)
                     /locus_tag="PLF85_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20950.1"
                     /translation="MKQLIALIHLLLIAHLAVACGKVTGKDELAKESGTAGVANPNSP
                     EGSTPGAPPAYVSHTRINPSLLEVTLTKELTDATATNTSNFIFNNGLQAVSVAKKAGE
                     PATLQITTTTQQQINYGLTILGVTTVEAVTLSGVIQITFEGLPSPAITSVDWRDPATP
                     ASALPANVYGISLCQLGSGAGSVCTTAPFYNRSALHGILTGGNAVAYSYKIDAGSWSA
                     ETPMATPLAVTGLADGFHTIYIIGKHTNGYWQSQTATDVFTLSWVQDTVAPEAYIDPL
                     TYPASVTASTSFNVRVIGTDVSFFRYCLDNGATGDCTANSWQGNPAPAASSGYALPIG
                     SYAGSLVPGAVTLKVIGIDRSGNVQSSAIAGGTYSYVVDTGSVEALFNASDVAAITTT
                     GTTVSVRITNAAGAVAYKGKIVSGTDCNAGTTWDLLPETALSTPITATGLSANGGYYT
                     VCAIGKSLGNNWQGGWTGAATANVVTKYSWIVDTTAPTAQITWLSPYELPTTTTETSY
                     QWQISTSDGVTHYRSAIVTGAGASCASATYDPETSVANAISFTAATTGVNTYKLCVIA
                     RDAAGNYQAIGSATSSGEWTVDKEAPGNNPAFASASQSARSFSVPVIQFACRAGLSRC
                     VL"
     gene            complement(2024..2797)
                     /locus_tag="PLF85_14600"
     CDS             complement(2024..2797)
                     /locus_tag="PLF85_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20951.1"
                     /translation="MRLITRKLLPTFVLAAAMTQAVVAQAAAKPAPAKPAGKKVDLKK
                     PTGKDAKKEETPPEQEFTGEQNAANNYGRPWKRSQLDLRVGLLGGPIIPIGQAGDVLE
                     TGWGGRLWASAETSFIKLPKSKIFENTLLIYEFSFYKVKGTSVTSKIGQDTDADFVTN
                     AGAIGKAYDVPFSPKFVMRFVPYFGFGLTNITSDTKLASGQTIRAVSGDIIMKYGVAA
                     AYDFNRRWQALLTLDHYIYFEEKIGMSLRINLGATMMIF"
     gene            complement(2935..>3527)
                     /locus_tag="PLF85_14605"
     CDS             complement(2935..>3527)
                     /locus_tag="PLF85_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNE20952.1"
                     /translation="KKQIEETTSDYDREKLQERLAKLAGGVAVIHVGAATEVEMKEKK
                     ARVEDALSATRSAVEEGIVPGGGLALLKAIEVVEKLKLEGDQETGKNIVMRSLEEPMR
                     QIATNSGLEGSLIVKQAKSEKDNIGFNAANLKWEDMLKVGIIDPAKVTRSALQNAASI
                     SGLVLTTECIISDKPEKKEAPAMPGGMGGMGGMGDF"
BASE COUNT          736 a          981 c          955 g          855 t
ORIGIN      
        1 ccagcacgca gcggctgagc ccggcgcggc aggcgaactg gatcaccggc acgctgaaac
       61 tccgcgcaga ttgtgaagct gaagcaaaag caggattatt gcccggagct tctttgtcga
      121 cggtccattc gccgctcgag gttgcactac cgatcgcctg gtaattacct gcagcatcgc
      181 gcgcgatgac acagagtttg taggtattca ccccggtggt cgcagctgta aagctgatgg
      241 cattcgccac gcttgtctca gggtcgtatg tggccgaagc gcacgaagcc ccggcacctg
      301 tgacgatagc agaacggtaa tgggtcacgc cgtctgatgt ggaaatttgc cattggtagc
      361 tggtttctgt ggttgttgtt ggcagctcat aggggctgag ccaggtaatc tgcgccgtgg
      421 gtgcagtggt atcgacaatc cagctgtact tggtgacaac attggctgtc gcggcgcctg
      481 tccaaccacc ctgccagtta ttgccgaggc tcttgccgat tgcacagaca gtgtagtaac
      541 cgccgttcgc cgaaagtccc gttgccgtaa tcggcgtcga caatgccgtt tcaggcagga
      601 gatcccacgt tgtcccggcg ttgcaatcgg tgccgctgac gatctttcct ttataggcta
      661 ccgctcccgc tgcattcgtg atgcgtacgc ttaccgttgt gccggttgtg gtgatcgcgg
      721 caacatcaga cgcattgaag agcgcttcga cagacccggt atcaacgacg tacgagtatg
      781 tgccgcccgc aatcgcacta ctctgcacgt tgcccgaccg gtctatgccg atcactttga
      841 gcgtgactgc gcccggtacg agactgccgg catacgatcc gatgggcagc gcataacctg
      901 agctggctgc cggtgccggg tttccctgcc agctattcgc cgtgcagtcg ccggttgcgc
      961 cattatcaag gcagtagcga aaaaacgaaa catcggtacc tatgacgcgc acgttgaagc
     1021 tcgttgatgc cgtaaccgaa gccgggtaag tcagtggatc tatgtacgct tcgggagcca
     1081 cggtatcctg cacccatgaa agcgtgaaaa catcggtcgc cgtctgcgac tgccagtaac
     1141 cattcgtgtg cttaccaatg atgtagatcg tgtggaaccc gtcagcaaga ccggtgactg
     1201 caagaggcgt cgccatcggt gtttcagccg accaggaacc agcatcgatt ttgtagctgt
     1261 acgctacggc attgccgccg gtcagaatac catgcagcgc actgcggtta tagaagggtg
     1321 cagtcgtgca gacagaacca gcaccgctgc ccagctggca gagtgaaata ccatagacat
     1381 ttgccggcag cgcactcgcc ggagttgcgg ggtcgcgcca atcaacactc gtgatcgccg
     1441 gagagggcaa accttcaaag gtaatctgga tcacacctga cagggtcaca gcctcaaccg
     1501 tggtaacacc aaggatcgtc agcccatagt ttatctgctg ctgcgtcgtc gtagtgatct
     1561 gcagcgtcgc gggttcgccg gctttttttg cgaccgaaac cgcctggagg ccattgttga
     1621 agatgaagtt cgaagtattt gtcgccgttg catcggtcag ctcttttgtc agcgttacct
     1681 caagcagtga cggattaatg cgcgtgtgcg agacatacgc aggtggcgct ccgggggttg
     1741 aaccttcagg tgagttcggg ttggcgacgc cggcggtgcc cgattctttc gccagttcgt
     1801 ctttgcccgt gactttaccg caggccacgg caagatgcgc aatcagcaag agatggatca
     1861 atgcgatgag ttgcttcata attgtcctct tctgtactct tagacgattc cttatctata
     1921 actacgacac ctgatgccgc caactcaaac cggctggcaa ccgtaagtct tgttgcacgc
     1981 cgtgcattat gtctccagct ttccggattt tgcctggggc gtcttagaaa atcatcatcg
     2041 tcgcgccgag attaatccgc agcgacatgc cgatcttttc ttcgaaatag atatagtgat
     2101 caagtgttaa caatgcctgc cagcggcggt tgaagtcgta agccgcagca actccgtact
     2161 tcatgataat gtcaccactc acggcgcgta tggtctgccc gctcgccagc tttgtgtcag
     2221 aggtaatgtt ggtaaggccg aagccaaagt atggcacaaa gcgcatgaca aacttgggcg
     2281 aaaagggcac gtcgtacgcc ttgccgatcg ccccggcgtt ggtgacaaaa tctgcatcgg
     2341 tatcctgacc gatcttactc gttacagacg tacctttcac cttatagaag ctgaactcgt
     2401 aaataaggag agtgttctca aagattttag acttcggcag cttgatgaaa ctggtttcag
     2461 cagaagccca gagcctgcca ccccacccgg tctcgagtac atcgccggcc tgcccgatag
     2521 gtataatcgg cccgccgagc agccccaccc gcaaatcgag ctggctgcgc ttccaggggc
     2581 gcccgtagtt gttggcggca ttctgctcgc cggtaaattc ctgctcaggg ggagtttctt
     2641 cttttttagc gtctttgccc gtgggcttct tcagatccac ttttttccct gctggttttg
     2701 cgggggcggg ttttgccgca gcctgcgcga cgacggcctg cgtcatcgca gcagcgagaa
     2761 caaacgtggg taatagtttt ctggtgatga gcctcatcgc tatttcttta tcggtactct
     2821 cccgtactca actctatgcg acgcaagaaa aaatacccat acaacaaaaa aaagggcccg
     2881 aaggcccttt ttgagctttt tggagtgttg ctgtgcaaca ctccaaaaag aagattaaaa
     2941 atctcccatt ccgcccatgc cacccatgcc gcctggcata gctggtgctt cttttttctc
     3001 tggtttgtca gagataatgc actcggtggt cagcacgagg cccgagattg aggctgcgtt
     3061 ctgcagagct gagcgcgtca ccttcgcagg gtcgatgatg ccgactttca gcatatcttc
     3121 ccacttgagg tttgcagcgt tgaaaccgat gttgtctttt tcgctcttcg cctgcttgac
     3181 aatcagtgaa ccttcgaggc ccgagtttgt cgcgatttgg cgcattggct cttcgagcga
     3241 gcgcatcacg atgttcttac cagtttcctg gtcgccttcg agtttcagct tctcaacaac
     3301 ttcgatcgcc ttgagcagtg cgagaccgcc gccaggaaca ataccttctt cgacagctga
     3361 acgagttgca gaaagagcgt cttcaacgcg tgctttcttc tctttcattt cgacttcagt
     3421 tgcagcacca acgtgaataa ctgctacgcc gccagcgagt ttcgccaggc gctcttgcag
     3481 tttttcacgg tcgtaatcag aagtggtctc ttcgatctgc ttcttga
//
LOCUS       DAOQJF010000344         3508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17033_length_3508_cov_6.100492, whole genome shotgun sequence.
ACCESSION   DAOQJF010000344 DAOQJF010000000
VERSION     DAOQJF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17033_length_3508_cov_6.100492"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLF85_14610"
     CDS             complement(<1..762)
                     /locus_tag="PLF85_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20953.1"
                     /translation="MTSMFAPRIGLLVCAALLALNCARKPTELNGAIKGKLSLRLANG
                     APISQPDTDEYNPQLILGPNNYLALVFGSNRSCALSCTGHNIFVSMSLTPFDGQSLPF
                     FSAPAVVEYSGSPWLNDPAPINFVAVLEGSALKVYANNSGVSDQLMVAEVNNSGTIPS
                     NPQVIANSFHSYSTVIGVDSSGQRLVVLDQATFEAYEIDPATSNQGTRLAAIDYAASA
                     VGVRQENSGLQDGYFASYTAFTFAASKDMFIGPLAS"
     gene            complement(862..1668)
                     /locus_tag="PLF85_14615"
     CDS             complement(862..1668)
                     /locus_tag="PLF85_14615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE20954.1"
                     /translation="MATTQRVAAPAALQLGTGTAVKPERQPVGSLVYRFKMMLIGIWA
                     AIVTFSVFVVSLFTWGHVVSGWLYARGLHLLGMPILGIKVKVSGRENLPQEPAVLLVN
                     HQSNFDAFFLGGLFPWKTVVVAKTQMQKVPLFGLTLRASRTIFVDREDSADAKKAIKD
                     AINAIKNDKNNIWIFPEGTRSQGKGLGEFKKGAFAMAIAAQAPIVPIVNQPMEHVLDT
                     QTKTLRGGVQHVKILPAIETKGMKFRDIDALLKRVEELFHEEIRNFSEVE"
     gene            complement(1738..2529)
                     /locus_tag="PLF85_14620"
     CDS             complement(1738..2529)
                     /locus_tag="PLF85_14620"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE20955.1"
                     /translation="MDLASTAAQAILYLLAISAVFIPLEYFWRADRFSSMRGFSWLRR
                     DWLNDLCFYLGQALLFNALTLFVLGYLFRGVNSTAYLPAWWLQAPSWLRVALVILLGD
                     ALIYWGHRAQHTIPVLWRFHRVHHTAEQVDYLAAYREHPLDNIYTRGLETLPAVLLGL
                     DLNTIAGFVTFRGLWALFIHSNTGIRLGLLEPLLGSPHLHHWHHEKTKGGFHNFANLS
                     PLLDIAFGTYYSPAHRPETYGIPERVRTEWPWQMIDPLLPVKQRE"
     gene            complement(2529..2888)
                     /locus_tag="PLF85_14625"
     CDS             complement(2529..2888)
                     /locus_tag="PLF85_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20956.1"
                     /translation="MATQQPPDSFFNREAFVTAADGTRCALDETDFTELNNRSNYWHS
                     REDPSTWLCVSTVSRARNKFRDKLNRQQTIALCAQTLNITPSKLEHALDWNANYMAWH
                     DGGSVEETHAWLPGSET"
     gene            complement(2934..3254)
                     /locus_tag="PLF85_14630"
     CDS             complement(2934..3254)
                     /locus_tag="PLF85_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20957.1"
                     /translation="MKATAMVARYLSLAFALLALAGAVIYAQKQGKENPRRDPVFDTS
                     KSGLPTPIDASERERERIRRENEDHANKNAGDKQQANPQQKNQVEDKWPEPREYPRKK
                     KGQR"
BASE COUNT          782 a          946 c          993 g          787 t
ORIGIN      
        1 cgaggcaagc ggaccgataa acatatcttt actggcagca aaggtaaaag ctgtgtacga
       61 ggcaaagtac ccgtcttgca ggccagagtt ttcctgcctc acaccgaccg cagaagctgc
      121 ataatcgata gccgcgaggc gcgtaccctg gttgctcgtc gctggatcga tttcgtatgc
      181 ctcaaatgtt gcctgatcca acaccaccag cctctggccc gaagaatcga caccgataac
      241 cgtcgagtac gaatgaaacg aattggcaat gacctgcgga tttgaaggaa tagtacccga
      301 attgttcacc tctgcgacca tgagctgatc gctgacaccg ctgttgttgg cgtatacttt
      361 gagtgccgaa ccttcaagta cagccacaaa gtttatcggc gcaggatcgt tcagccacgg
      421 gctgcccgaa tattcaacca ctgcaggagc gctgaaaaac ggcagggatt gtccgtcaaa
      481 tggcgtcagt gacattgaaa caaagatatt gtgcccagtg cagctgagtg cgcaactcct
      541 gtttgaacca aagaccagcg cgaggtaatt attcggcccc agaatcaatt gggggttgta
      601 ctcatcagtg tcaggctgcg aaatgggagc gccatttgcc aggcgcaatg acagcttgcc
      661 cttgatggcc ccgttgagtt cagtgggctt gcgcgcgcag ttaagagcga gcagcgctgc
      721 gcataccagt agacctatgc gaggtgcgaa catcgaagtc atcgcacaac acagatgagc
      781 gatacgcggt ggcaagattt tacgaagtac tgcgcgagac gactgaaaat agccggttca
      841 aaaagccggc cagagtcttg attactcaac ttccgaaaag ttgcggattt cctcgtgaaa
      901 cagttcttca acgcgcttaa gcagagcatc gatgtcacgg aatttcatgc ccttcgtctc
      961 aatcgccggc agtattttca catgctgcac gcccccgcgc agtgttttgg tctgcgtatc
     1021 gagcacatgc tccatcggct ggttgacgat cggcacaatc ggcgcctgcg cagcaatggc
     1081 catggcgaaa gcgcctttct tgaattcgcc caggccctta ccctggctgc gtgtaccttc
     1141 gggaaaaatc cagatgttat tcttgtcgtt cttaatagcg ttgatcgcgt ccttgatcgc
     1201 cttcttcgcg tcggcactat cttcacgatc cacaaatatc gtgcgagaag ccctcagcgt
     1261 taacccgaaa agcggaacct tttgcatctg cgtttttgcg accacaacgg ttttccatgg
     1321 aaacaatcca cccaggaaga aggcgtcaaa gttggattga tggttgacca gcaggactgc
     1381 cggctcctgc ggcaggtttt cgcgaccact gactttgacc ttgatgccca gaattggcat
     1441 acccagcaga tgcagcccgc gtgcatagag ccagccgctg acgacatgcc cccacgtgaa
     1501 cagactcaca acaaacaccg aaaaagtgac aatcgccgcc cagatgccaa tcagcatcat
     1561 tttgaaacgg taaaccagcg acccgacagg ctgccgttca ggtttgacgg ccgtgccggt
     1621 acccagttgc agcgccgctg gtgcggcgac tcgttgcgtg gttgccatag ctatgagatg
     1681 accgcagcaa aaggtgagtt gcaattttct tctttgggca gtcttctgct gcctgcttca
     1741 ctcacgctgc tttacgggca gcagtggatc gatcatctgc cagggccact ccgtccgcac
     1801 gcgctcggga attccatatg tttcagggcg atgcgctggg ctatagtagg tgccgaaggc
     1861 gatgtccaaa agcggcgaaa ggtttgcgaa attatgaaat ccccctttcg tcttttcatg
     1921 gtgccagtgg tgcaggtggg gtgaaccgag taaaggttcg agcagaccca acctgatacc
     1981 tgtgttcgaa tggataaaga gcgcccacag gccacgaaag gtcacgaagc ccgcgatcgt
     2041 attgagatcc agccccagga gcactgccgg cagcgtctcg agcccgcgcg tgtagatgtt
     2101 gtcgagcggg tgctcgcggt aagcggcgag ataatcaacc tgctctgccg tgtggtgcac
     2161 gcggtgaaag cgccagagaa caggtattgt atgctgcgcc cggtgtcccc agtagatcag
     2221 tgcatcgccg agcaggatca cgagtgctac tcttaaccag gaaggtgcct gcaaccacca
     2281 tgcgggcagg taggcggtgg agtttacacc gcgaaagagg tagcccagta caaacagcgt
     2341 gagcgcattg aacagcagag cctgcccgag gtagaagcag agatcgttca gccagtcacg
     2401 gcgcagccac gagaaccccc gcatgctact gaatctatca gcgcgccaga agtactcgag
     2461 tggaatgaac acggcgctga tcgcaagaag atagagtatt gcctgcgccg cggtggaggc
     2521 gagatccatt aggtttcact acctggcagc caggcatgtg tttcttcaac cgagccaccg
     2581 tcatgccagg ccatgtaatt ggcattccag tcgagcgcat gctcgagttt tgagggtgtg
     2641 atgttgagag tctgcgcaca aagggcaatc gtctgctgcc ggttgagctt atcgcgaaat
     2701 ttgtttctgg ccctgctgac cgtcgagaca cacaaccatg tagaaggatc ttcgcggctg
     2761 tgccagtagt tcgagcggtt gttgagttca gtaaaatcgg tttcatccag cgcacaccgc
     2821 gttccatcag ccgcggtcac aaatgcctcg cggttaaaga aagaatctgg cggctgttgt
     2881 gttgccatga ccgaaagctg cccggggttg ttgccgattg gggatagcca gtgttagcgc
     2941 tggccctttt tcttgcgcgg gtactccctg ggttcaggcc atttatcttc tacctggttt
     3001 ttctgctgcg ggtttgcctg ctgcttatca ccagcattct tgtttgcatg gtcttcattt
     3061 tcacggcgaa tacgctcgcg ttcacgttcg ctcgcatcga tcggcgtggg caagcctgat
     3121 ttgctggtat caaagaccgg gtcgcgccgc ggattttctt tgccctgctt ctgtgcgtag
     3181 atgaccgcac cggcgagagc cagcagggca aaggcgagac tgaggtagcg tgcgaccatg
     3241 gctgttgctt tcatgttgag gtgggcttac aagccgaaat tggcgggtcg cggctcctcc
     3301 tcttcattta atggagaggg ggttgggggg tgaggcggta aactgcaact acgacgcggt
     3361 cgcgaagttt ggtctcgaca cctcgaagat gttatcccac ccgaaatcgg tcttggcgcg
     3421 tatatcgcgg tgccagtgtt cgtccttcca gccgcccttg agcgactgcg cgagcaggta
     3481 agcgggtgat aggcccgcac tgtcggct
//
LOCUS       DAOQJF010000345         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17115_length_3499_cov_4.545006, whole genome shotgun sequence.
ACCESSION   DAOQJF010000345 DAOQJF010000000
VERSION     DAOQJF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17115_length_3499_cov_4.545006"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /gene="fliD"
                     /locus_tag="PLF85_14635"
     CDS             <1..1619
                     /gene="fliD"
                     /locus_tag="PLF85_14635"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNE20958.1"
                     /translation="YHGKQLSAKIVQVKENESILILDTLAQGEDALLKIEDPDGLLRS
                     LGLLGVSATPEPGGKADEPKKAPPADPAKPAETTEKERWVLNPQQLEPVKGAAPVASE
                     DRRILEVAEGSVGRYRLLAGQNSGDRRLQSVSLAAMARADAPSADDAPDHLTDGIKDN
                     INIKGIELETYNITRTRKKDPMQSGDYGVILRYGEREQSVSLKGKTGAQVLPVEAGLT
                     AVDFYSVGEDVLFEPLELSYAVTPQPKIDHSKAEKDKTPEEAEQRRMFPNLLRPAQNA
                     KLKIDGIDISRKSNSGLNDIIEGVSLNLLKTSPDNVETEILNDNDAAKKQVLGFVKAY
                     NDLLQFSEEVAKTAKIKEAGKYREMRAESGILATNATIRQLVNGLKMHTSNAYPANRE
                     PYIRTLTQIGISSGAIGGKREEVTKGYLEVDETKLAQMLAENPAAVKELFAIDTNGDL
                     RIDNGYAHVTENFLEPFTRFTRGLISEQIKSNQERVKQIDRDIKRQEEHVKTYEAKLK
                     TKFGYMESSVQKSKSTGTFLKQKLGGGDGR"
     gene            1659..2567
                     /locus_tag="PLF85_14640"
     CDS             1659..2567
                     /locus_tag="PLF85_14640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE20959.1"
                     /translation="MGKIRVSVVTPTLNEERDLPQLLSSLKKQTFRDFEIIVADAGSK
                     DRTRQLAEEYGARVVDGGMPGVGRNRGAAVANGEYLFFFDADVVLPDDFLFKAVQEMD
                     EQYIDLATCAFYPISDLRLDKILFAFTNLTVRMSANSNPRAAGFCIFITKRLFDRIGG
                     FDESLRLAEDHDLVERAAKFRPLHVLKSTSLQVSVRRLAKEGRFSLIQKYFQVEMHLM
                     TKGKVRDDIIEYEFGNFQDESTETVKKAMDQFEDRLIKLEAQYNDWSQKVQTLPVVER
                     MKETQQRLAQSAEAVSTSLRELFSAK"
     gene            complement(2560..>3499)
                     /locus_tag="PLF85_14645"
     CDS             complement(2560..>3499)
                     /locus_tag="PLF85_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNE20960.1"
                     /translation="DIIRHFSDYTTLVIEKLGDLINDFIVLNEPMIFLTLGYLLGLHA
                     PGKRSIKKFFAASHHALLAQAEAARTIRAAIPGARIGTTVSTTAVYPASERPRDLLAA
                     ERFDVLYNTFYVDPVVGRGYPTRHFPFLRRIEKYVQPGDMEALRFDFDFWGINTYTRK
                     VVRYSRITPVIHWRELKNPPGAAANCMNWEVYPEGLYDLLKKFSAYPEIRELMVTENG
                     FAAEDQLVAGRVADAERIGYYREYIGQVLRAKQEGVNVTGYFAWSLLDNFEWAEGYRP
                     RFGLVHVDYLTQKRTIKDSGYWFRDFVRGRAPDKIT"
BASE COUNT          869 a          926 c         1004 g          700 t
ORIGIN      
        1 gctaccacgg caaacaactc agcgcgaaga tcgtgcaggt caaagaaaac gaatctatac
       61 tcattctcga tacgctggcg cagggcgaag atgcgctgct gaaaatcgaa gatcccgatg
      121 gtctgttgcg gtcgctggga ttactgggtg tttccgcgac gccggaaccg ggcggcaaag
      181 cggatgagcc gaagaaggcg ccgccggctg accctgcgaa gcctgccgag accaccgaaa
      241 aagagcggtg ggtgcttaac ccccagcaac tggagcctgt taaaggagct gcccccgtcg
      301 cctcggaaga ccggcgcata cttgaagttg ccgagggttc cgtggggcgt taccggctgc
      361 ttgccgggca gaattccggt gaccggcgcc tgcaatcggt ttcgctggcc gcgatggcac
      421 gtgcagatgc gccgtctgcg gacgatgcgc ccgaccattt gaccgacggt attaaagata
      481 acatcaacat caaaggtatc gaactcgaga cctataatat cacaaggacg cgcaagaaag
      541 atccgatgca gtcgggcgat tacggggtga ttctccggta cggcgaacga gaacaatctg
      601 tttcgctcaa aggtaaaacc ggtgcacagg tgctgccggt cgaagctggt ctcaccgcgg
      661 tggatttcta ctcggtgggt gaagatgtac tttttgagcc gctggagctc agttatgcag
      721 ttactcccca gcccaagatc gatcattcga aagccgagaa agacaaaact ccggaagagg
      781 ctgagcagcg gcgcatgttt cctaacctgc tgcggccggc gcagaatgcc aagcttaaga
      841 tcgacggcat cgatatcagc aggaaatcca actcgggttt gaatgatatc atagaaggag
      901 tttcgctgaa cctgctgaaa acctccccgg acaacgtgga gaccgaaatt ctgaacgaca
      961 atgacgctgc aaaaaagcag gtgctcgggt tcgtcaaggc atacaacgac ttgctgcagt
     1021 tttctgaaga agtggcaaag actgcgaaga taaaagaagc gggtaaatac cgtgagatgc
     1081 gcgccgaatc gggtatactc gcaaccaacg cgactatcag gcagctcgtc aatggcctga
     1141 agatgcatac ttctaacgcg tacccggcta accgggagcc gtacatccgc acactcacgc
     1201 agatcggcat atcgagcggt gcgattggcg gcaaacgcga agaagtgacc aagggttacc
     1261 tcgaggtcga cgaaacgaag ctggcacaaa tgctcgctga gaatccggct gccgtgaaag
     1321 aactttttgc tatcgatacc aacggcgacc tgcgcatcga caacggttat gcgcacgtga
     1381 cagagaattt tctcgaaccc tttacgcggt tcacgcgcgg cctgatttca gagcagatca
     1441 aatcgaacca ggaaagggtg aagcagatcg accgcgacat caagcggcag gaagaacacg
     1501 tcaaaaccta cgaagccaaa ctcaagacca agttcggtta tatggaaagt tcggtacaga
     1561 aaagcaaatc aaccggcact ttcctcaagc agaaactcgg tggtggcgac ggccgctaat
     1621 tgactcgacg gaaagactcg gccgcttaag gctggcggat gggaaaaatc cgggtcagtg
     1681 tggtaactcc cacactcaat gaagagcgcg atcttccgca gctgttgtca tcgctcaaaa
     1741 agcagacttt ccgcgacttt gaaatcatcg tagccgatgc cggctccaag gaccgcacgc
     1801 ggcaattggc cgaagaatac ggggcgcgcg tcgttgacgg aggtatgccg ggtgtggggc
     1861 gcaaccgcgg ggcggctgtc gccaacgggg aatatctctt tttcttcgat gcagacgttg
     1921 tgttgccaga tgattttctt tttaaggccg tgcaggaaat ggatgaacag tatatcgatc
     1981 ttgctacctg cgccttttac ccgatctcag accttcggct ggacaagatt ctctttgcct
     2041 tcacaaatct gacagttcgc atgagcgcga acagtaaccc gcgtgcagcc ggtttctgca
     2101 tctttattac caaacgcctg tttgaccgta tcggggggtt cgacgagagt ctgcgcctgg
     2161 cagaagacca tgacctggtc gaacgcgcgg caaagtttcg gccgttgcac gtgctgaaat
     2221 cgacttcgct ccaggtgagc gtgcgccgcc tcgccaaaga aggccgcttc tctctcatcc
     2281 agaaatattt tcaggtagaa atgcacctga tgacgaaagg caaagtgcgc gacgacatca
     2341 tcgaatatga attcgggaac tttcaggacg agagcactga aacggtgaag aaggcgatgg
     2401 accagttcga agaccggctc atcaagctgg aggcgcagta caatgactgg tctcaaaaag
     2461 tccagacgct tccggtagtc gagcgcatga aagaaacgca gcagcgtctg gcgcaaagcg
     2521 cagaagccgt ctctaccagc ctgcgcgaat tattttccgc taagtgattt tgtcaggggc
     2581 tctgcccctg acaaaatcac gaaaccagta gccgctgtct ttgatcgtgc gtttctgcgt
     2641 caggtagtcg acgtgcacca ggccaaagcg gggacgataa ccttccgccc actcgaagtt
     2701 gtcgagcagc gaccaggcga agtaacctgt gacgttaacc ccttcctgct ttgcgcgtaa
     2761 cacctgcccg atgtattccc ggtaataacc aatacgttcg gcatcggcga cgcggccggc
     2821 aaccaactgg tcttctgcgg caaaaccgtt ttcggttacc atcagttcgc gtatttcggg
     2881 gtaggcggag aacttcttga gcaggtcgta gagtccctcg ggatagacct cccagttcat
     2941 gcagttcgcc gctgcgccgg gcgggttctt cagctcgcgc cagtggatga ccggggtgat
     3001 acggctgtag cgcaccacct tgcgcgtgta ggtgttgatg ccccagaaat caaaatcgaa
     3061 acgcagcgct tccatatcgc ctggctgcac gtatttttca atgcgccgca aaaacggaaa
     3121 atggcgagtg gggtagccgc gaccgacgac ggggtcgacg tagaaggtgt tgtacagcac
     3181 atcaaaacgt tctgcggcga gcaggtcacg cggccgttca ctcgcgggat aaaccgcagt
     3241 cgtagagacc gtcgtgccga tacgggcgcc ggggatcgcc gcgcgaatcg tgcgtgcggc
     3301 ttcggcctgt gccagcagtg cgtgatggct cgcggcaaaa aacttcttaa tagatcgttt
     3361 accgggagcg tgcaggccga gcaggtaacc aagggtcaaa aagatcatgg gttcgttcag
     3421 cacgatgaag tcatttatta ggtctccgag cttttcgata accagcgtcg tgtagtcgga
     3481 aaaatgcctt atgatatca
//
LOCUS       DAOQJF010000346         3483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17240_length_3483_cov_5.148775, whole genome shotgun sequence.
ACCESSION   DAOQJF010000346 DAOQJF010000000
VERSION     DAOQJF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17240_length_3483_cov_5.148775"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..2704)
                     /locus_tag="PLF85_14650"
     CDS             complement(308..2704)
                     /locus_tag="PLF85_14650"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNE20961.1"
                     /translation="MSNNGAQLIFSELLTRHGRIQVPLIQRDYAQGRADQQEVREEFL
                     AALHEALRLPPEAESLPLNLDFIYGSVEGQVASCFQPLDGQQRLTTLFLLHWYLAWRD
                     GRGDEFRALCTDEAGSRFSYHVRPSSSEFFDALVAFQPRRSPDDVTAVSVLIIDQPWY
                     FRNWRLDPTIQSALVMLDDLHKYFRSEQGLYARLIDTERPAITFQLLDLKHFGLSDDL
                     YIKMNARGKPLTPFETFKARYEQTLKDLFPKEQRVLDGQYVSMAEFFARRMDTSWADF
                     FWPHRDPNSNVFDAAVMNVFRAVILITRSPQADGFGEDVNLLRNRFRKNSYAFFHQRD
                     WLDRPFSEMLAVLLEAWSAAGSDIGMQLPDGRYFNEKAVFDKLLSDPTTLGYEDIVQL
                     AAYGQYLKTHGDKFESTDFQAWMRVVFNLSVNTEYNRPADLQRSLAALNTLAPHMTSI
                     LDYLARADSEITGFNQQQVAEEKLKAKLLIADNTWRVLIEQAEGHGYFRGQIGFLLAF
                     SGVSVHPAAEGEEPWTHETTLRLQHEFKGVLQQASAMFGDSGLNSLPDYRWERALLAL
                     GNYLLAARRNDSFLVNAQAEPASWKRLLRGAWSGSPQAAVLCSLWKRLQGTANLEAQL
                     DAVIAEASGIEDWRAALIATPAAIGYCHQRMIRHAENARTYLLKKLQMNGAHAELFTY
                     CLYAQTLQSADREGRLQVLTPYYEETNTAEEEPHVRLRGHHGDRLLIFVIEFDYVQYE
                     IKLLEPHPEFLPDPLLALFQKIGFALTGTHLVKHSTIDNIESDIFELDRHLQIMRRNV
                     "
     gene            complement(2701..>3483)
                     /locus_tag="PLF85_14655"
     CDS             complement(2701..>3483)
                     /locus_tag="PLF85_14655"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HNE20962.1"
                     /translation="INALLEAAVTRRKAEFAGELRRRAFALAIGADLAALDDGQLTAR
                     IRERCEGVSYARDPGAIRTLLLLFNLATLLENPRSNLRFQFDSFKRENWDIEHVRAVA
                     ARRPERHNERLEWLGHCQGYLQTREDGQALAQAIEDFVALPQRSAVDALFDPLYEDVL
                     IYFHESDEGEENGLSNLTLLDRSTNRSYKNAVFALKRQQLLELDQSGIFVPLCTRNVF
                     LKCYSPQVDNAMFWSQEDANAYGDVIVETLRRFFCSGREVQV"
BASE COUNT          785 a         1014 c          920 g          764 t
ORIGIN      
        1 agaacaccgg tcacgttcgt cggtatgagc ggtcacgatc gtcggaatgc gcaatcgagt
       61 gcagccgacc cgttgcggcc tgatggcttc ttcatatcct atggcagcta tgtgaccctc
      121 aagcttcctg tcggatctag gcctacggta tttgactccg cccgtaaggg cgtttaattt
      181 cgcatgagcg aatggcccaa ccgtgaaccg tttgcagcca cggaccaaat aacattcata
      241 cagtttttct agaccaatca cgtttcattt atttggtttc cgcagtgtgc aatattatct
      301 tcatgcgtta aacgttacgt cgcataattt gcagatgtcg atccagctcg aaaatgtcgc
      361 tctctatgtt atcaattgta gaatgtttca caagatgcgt tccggttagg gcgaagccaa
      421 ttttctgaaa caacgctaac aggggatcag gcaggaattc gggatgtggt tccagcagtt
      481 tgatctcata ttgcacgtaa tcgaactcga taacgaaaat cagcagccga tctccatggt
      541 gacctcggag tcgtacgtgt ggctcctctt cagcagtatt tgtttcctcg taatagggcg
      601 tcagcacctg tagtcggcct tcccgatcgg cagactgaag cgtctgagcg taaagacaat
      661 aggtaaacag ctcggcatga gcaccgttca tctgcaactt ctttaggaga tatgtcctgg
      721 cattctccgc atgacgaatc attcgctggt gacagtaacc aatggccgca ggagtggcaa
      781 taagtgcagc gcgccagtcc tcgatcccag aagcttctgc aatcacggca tcgagttgcg
      841 cctcgagatt cgctgtgccc tgtaggcgtt tccaaaggga gcacagcact gccgcctgcg
      901 gcgaccctga ccacgcgccc ctgagcaagc gtttccaact tgcaggttcc gcttgtgcat
      961 tgaccagaaa cgaatcgttg cggcgggccg ccagcaggta attgccgagt gcgagcaatg
     1021 cgcgttccca acgatagtct ggcaggctgt ttaggccgct gtcgccgaac atggcggacg
     1081 cctgctgcag cacacctttg aactcatgct gcagcctcag ggtcgtctca tgagtccacg
     1141 gctcctcccc ctcggctgcc ggatgtacgc tcactccgga aaacgccagc aggaatccga
     1201 tctgtccgcg aaaatagccg tgcccttccg cctgttcgat caagactcgc caggtgttgt
     1261 cggcaattag cagtttcgct ttgagcttct cctcggccac ctgctgctgg ttaaagccag
     1321 tgatctccga atcggcccgg gccagataat ccagtatcga ggtcatgtgc ggcgccaacg
     1381 tattgagtgc ggccaggctg cgctgcaggt ccgcggggcg gttgtattcc gtattcacgg
     1441 acaggttgaa aacgacccgc atccatgcct gaaaatccgt cgattcgaat ttgtctccgt
     1501 gcgtcttaag gtactgtcca taggccgcca gttgaacgat gtcctcatag ccaagcgtag
     1561 tggggtctga taacagcttg tcgaaaacag ccttttcgtt gaaatagcga ccgtccggca
     1621 actgcatgcc tatgtcactg ccagccgcgc tccaagcttc cagcaacacc gccagcatct
     1681 cagagaaagg tcggtcgagc caatcccgct gatggaagaa cgcatagctg ttctttcgga
     1741 agcgattacg caggaggtta acgtcctccc cgaaaccatc ggcctgcggt gaccgggtta
     1801 tcaaaatgac cgcccgaaag acattcatca ccgcagcatc gaacacattg ctatttggat
     1861 cgcgatgggg ccagaagaaa tctgcccaac tagtatccat gcggcgcgcg aaaaattctg
     1921 ccattgagac atactgcccg tcgagcacgc gctgctcttt cgggaataga tccttcagcg
     1981 tttgctcgta gcgcgccttg aaagtttcaa atggggtaag cggcttgccg cgcgcattca
     2041 tcttgatata gaggtcgtcc gaaagtccga agtgcttaag gtctagcaat tgaaacgtga
     2101 ttgccgggcg ctcagtatcg atcaatctgg catacaggcc ctgctcactt cggaagtact
     2161 tatgaagatc gtcgagcatg accaacgccg actgaatcgt cggatcgagc cgccagttcc
     2221 gaaaatacca aggctggtcg attattagca cactcaccgc agttacatca tcgggggatc
     2281 gtcggggctg gaaagccacc agcgcatcga agaactcgct gctgctgggc cgtacatggt
     2341 agctaaagcg cgagcccgct tcgtcagtgc acagcgcgcg aaattcatca ccacgtccat
     2401 cgcgccaagc caaataccaa tgcaacaaaa acaatgtggt tagccgctgt tggccatcaa
     2461 gtggctggaa gcatgaagct acctgcccct caacactgcc gtagatgaaa tccagattca
     2521 gaggtaggct ctccgcctcc ggaggcaggc gcaaagcctc gtgcagcgcg gcaagaaact
     2581 cctcgcgcac ctcttgctga tcagcccggc cctgagcata gtcccgctga atcaggggca
     2641 cctggatgcg accatggcgg gtgagcaatt cactgaagat caactgggca ccgttattgc
     2701 tcatacctgc acctcccgac ctgaacagaa gaagcgcctc aaggtctcga cgattacatc
     2761 accgtacgca ttagcatcct cttggctcca aaacatcgcg ttatctacct gcgggctgta
     2821 gcacttgagg aacacattgc gagtgcacag tggcacgaaa atgccggact gatccagttc
     2881 caacaactgc tgacgcttca aggcgaacac tgcattcttg tagctgcgat tggtgctgcg
     2941 gtcgagcagc gtcagattgg acaggccgtt ctcctcccct tcatcgctct cgtgaaaata
     3001 gatcagcacg tcctcgtaga gcgggtcgaa caaagcgtcc accgcgctgc gctgcgggag
     3061 cgcaacgaag tcttcgattg cttgcgcgag cgcctgcccg tcctctcggg tctgcagata
     3121 cccctggcag tggcctagcc actccaaccg ctcgttatga cgttccgggc ggcgtgctgc
     3181 cacagcacgg acatgctcga tatcccagtt ttcgcgcttg aagctgtcga actggaagcg
     3241 caggttcgag cgtggatttt ccagcagcgt tgccagatta aacaataaca acaaggtgcg
     3301 aatcgccccc ggatcgcggg cgtagctgac gccctcgcaa cgctcgcgga tacgtgccgt
     3361 taattggccg tcgtccagtg ccgccagatc ggcgccaatc gccaaagcaa aggccctgcg
     3421 gcgcaactct cccgcgaact cggccttacg acgtgtcacg gccgcctcca gcagcgcgtt
     3481 gat
//
LOCUS       DAOQJF010000347         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17282_length_3477_cov_5.069842, whole genome shotgun sequence.
ACCESSION   DAOQJF010000347 DAOQJF010000000
VERSION     DAOQJF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17282_length_3477_cov_5.069842"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLF85_14660"
     CDS             <1..566
                     /locus_tag="PLF85_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20963.1"
                     /translation="EKKHNHANREHNRDGADHNHSFNCGAEGPTTDPGILALRAKQRR
                     NLLLTLFCAQDIPMLLAGDEIGHSQRGNNNAYCQDNKISWLDWHAQDRDLLSFTRQLA
                     AIRPQLLEVNLPQHVEAPTHHAQAFGIRFDNYLLLLNASQDNIMFPLPGITVREVLHS
                     GRPAQDDPLSDIVYVTAQSSVLFEVVR"
     gene            611..2686
                     /locus_tag="PLF85_14665"
     CDS             611..2686
                     /locus_tag="PLF85_14665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNE20964.1"
                     /translation="MTSVNPLLNQGELPDFGQIEAAHVLPAVREAIRLGREELSAIEK
                     ISDPTWENFVLPLRALSRRLDRVWGVVGHLMGVKNSDALRAAHEEAEAEIVQFRLELA
                     QSKAVFGLWQKLQNKQATELSSPARRRIVEAALRDARLAGVGLEGEKRDRFNAIQQSL
                     ASLSTKFSNAVLDSTKAFRLLLKSEDEVRGLPQSWRELAAQAARAGGETAASAENGPW
                     LLTLDFPSYYPFLQHAQNRAVREQVYRASVTRASGSGFSSQWNNWPLIDEILKLRREE
                     SGILGFSNFAEVSLAGKMAPDTAKIRAMITDLYKVAKGAAQKEISSLADYAKGKGLSG
                     DLSLWDISYYAERRREELYNYTDEELKPYFPLPRVLSGLFALSEQLFGIRVRPAEKTP
                     PLWHKDVQFFEVYSGDRKIAAFYLDPYARSGEKRGGAWMDVCRQREATADNTVLPVAY
                     LVCNGTPPVGSTPSLMTFSEVETLFHEFGHGLQHMLTTISEYEASGISNVEWDAVELP
                     SQFMENWVYERTVMDRISGHWQTGEVLPDALFQKIVAAKNYMAASQMLRQLYFSLLDI
                     ELHEKYDGTQSVREIQRRIAADYTVVPPLEEDAFLCSFSHIFAGGYAAGYYSYKWAEV
                     LSADAFAAFEEAGLANTDALQRLGRKYRDTVLSLGGSKHPLEVYREFRGRDATIEALL
                     RHNGLTSAA"
     gene            complement(2734..>3477)
                     /locus_tag="PLF85_14670"
     CDS             complement(2734..>3477)
                     /locus_tag="PLF85_14670"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNE20965.1"
                     /translation="RRELLRRLHSRPLIRFDGQFYPAWEFEQRLASADSLPADARDRL
                     LAHVHGLKQSEQPVQHEQGRRILTALKEGCGMLPCAEDLGVVPDYVRPALADMGILCL
                     KVYRWEAENNVPKDPSGYPYLSLATSSVHDSSNLREWLASEPQHITGVTVEDGKATAE
                     QVSDFLRRLYTSPSMIVMIPLQDLLALDESLHGDVKNERINVPGTVSDFNWTYRMPLS
                     LEALLTNRHLTSRVRQLAAARPKGAALPH"
BASE COUNT          734 a         1044 c         1019 g          680 t
ORIGIN      
        1 acgagaagaa acataaccac gcgaaccgag aacacaaccg cgacggcgcc gaccacaacc
       61 acagctttaa ctgcggcgct gaaggcccaa ccaccgaccc cggtatcctg gcgctgcgcg
      121 caaagcagcg ccgcaacctg ctgcttaccc tgttctgcgc acaggatatt cctatgctgc
      181 ttgccgggga tgaaatcggc cattcgcagc gcggcaacaa caatgcctac tgccaggaca
      241 acaagatctc gtggctcgac tggcacgcac aggaccgtga cctgctttct ttcacgcggc
      301 aactcgcagc aatcaggccg cagctgctcg aagtgaacct gccgcagcac gtggaagctc
      361 ccacgcacca cgcccaggcg ttcggcattc gcttcgacaa ctacctgctc ctgctgaacg
      421 cctcgcagga taatatcatg tttccgctac ccggcatcac cgtacgcgag gtactgcatt
      481 cggggcgccc cgcgcaggac gacccgttga gcgacatcgt ctatgttaca gcgcagagtt
      541 ctgtactgtt tgaagttgtg cggtgaggtg tagacggtcg cccctttctt ttgtaaattg
      601 cctgtgatat atgacatcag ttaacccatt gctgaaccag ggcgagctac ccgattttgg
      661 ccagatcgaa gcggcacacg tactgcctgc ggtgcgcgag gctatcaggc tcggccgtga
      721 agagctttca gcaatcgaga aaatctcaga cccaacatgg gagaattttg tcctgccgct
      781 cagggccctg tcgcgccgac tcgaccgggt ctggggtgta gttggtcacc tgatgggggt
      841 caagaactcc gatgcgctgc gcgctgcgca cgaagaggcc gaagccgaga tagttcagtt
      901 tcgcctcgag ctagcgcaga gcaaggccgt attcgggctc tggcagaaac tgcaaaataa
      961 gcaggcgacc gagctcagca gtccggcgcg caggcgcatt gtcgaggccg cgctgcgcga
     1021 tgcacggctt gcaggtgtcg ggcttgaagg agagaagcgc gaccgcttca atgcgattca
     1081 gcagtcgctc gcatcgcttt cgacgaaatt ttccaacgcg gtactcgact cgaccaaggc
     1141 ctttcggctg ctgctcaaaa gtgaagacga ggtgcgcggc ctgccacaat cgtggcgtga
     1201 gctggcagcg caggcagccc gcgcaggcgg cgagacagcg gcatcggccg aaaacgggcc
     1261 atggctgctg acgcttgact ttccttcgta ctaccctttc ctgcagcacg cacagaaccg
     1321 ggcggtacgc gagcaggttt accgcgccag tgtcacgcgc gcgagcggca gcggattttc
     1381 ttcgcagtgg aacaactggc cactgattga cgaaatactc aagctgcggc gtgaagaatc
     1441 aggaattctt ggcttcagca acttcgccga agtttcgctg gcaggcaaga tggcgcccga
     1501 taccgcgaag atacgcgcga tgatcaccga tctctacaaa gtcgcgaagg gcgccgcaca
     1561 gaaagaaatc agttctctcg ccgattacgc aaagggcaag ggactttccg gcgacctcag
     1621 tttatgggat atctcgtact acgctgagcg gcggcgcgag gaactctaca actatactga
     1681 cgaagaactc aaaccgtact ttccactgcc ccgcgtcctt tccggtctct tcgcgctgtc
     1741 agaacaactt ttcggaatcc gggtgcgccc cgccgaaaag actccgccgc tttggcataa
     1801 ggacgtacag ttctttgaag tctattcggg cgaccgcaag atcgcggcct tctacctcga
     1861 cccttacgcg cgctcgggcg agaaacgtgg tggcgcctgg atggacgtct gccgccaacg
     1921 cgaagcaact gcagacaata cggtgctgcc agtggcctac ctggtctgca acggcactcc
     1981 accagtgggg agcactccct ctctcatgac tttcagcgag gtggagacac tgttccatga
     2041 gttcgggcac ggcctgcagc acatgctgac gacaatatcc gagtacgaag cttcgggaat
     2101 ctcgaacgtc gaatgggatg ccgtcgaact gccgagccag ttcatggaaa actgggtcta
     2161 cgagcgcacg gtgatggacc gcatttcagg ccactggcag acgggcgaag tattgcccga
     2221 tgcacttttt cagaaaatcg tcgcggcaaa gaactacatg gccgcctcgc agatgctgcg
     2281 ccagctctat ttttcgctgc tcgatatcga gctgcacgag aagtacgacg ggactcaatc
     2341 ggtgcgcgag atacagcgcc gtattgccgc agactacacc gtcgttccgc cgctcgaaga
     2401 agatgctttt ctctgctctt tctcgcacat cttcgcgggc ggttacgcgg ccggctacta
     2461 cagctacaag tgggccgagg ttttgagcgc cgacgcattc gccgcctttg aagaggcggg
     2521 gctggcaaac accgacgcgc tgcagcgcct cggcagaaaa taccgcgaca ccgtgctttc
     2581 gctcggtggg tcgaaacacc cgctcgaagt ctaccgcgaa ttccgtggcc gcgacgcgac
     2641 cattgaagcg ttgctcaggc acaacggcct gaccagcgct gcctgaccag cgtagttcgg
     2701 gcaccaggct tcggtttggc ggaacctgct tcgctagtgc ggcagagctg cgccctttgg
     2761 acgggctgct gccagctgtc tgacccggct ggtcaggtgc cggtttgtga gcagagcttc
     2821 gagtgaaagc ggcatgcggt aggtccagtt aaaatcagac accgtgccag gcacgttgat
     2881 acgctcgttt ttcacatcgc cgtgcaggct ctcgtccaat gccagcaggt cctgcagtgg
     2941 aatcatgaca atcatcgaag gtgatgtata gagccgccgc agaaaatctg atacctgctc
     3001 tgccgttgcc ttgccgtctt cgactgtgac gccggtaata tgctgaggtt cactcgcgag
     3061 ccattcgcgc aggttggaag aatcgtgtac cgagctcgtc gccagtgata ggtaagggta
     3121 accggaaggg tctttgggta cgttgttctc tgcctcccag cggtagacct tcaggcagag
     3181 tatacccata tcggcaagcg cggggcgcac gtagtcgggc accacaccca gatcttcggc
     3241 gcagggcagc atgccacaac cttctttcag cgcggtgaga atgcgccggc cctgttcgtg
     3301 ctgcaccggt tgttcgctct gcttgagccc gtgcacgtgc gcaagcagcc tgtcgcgcgc
     3361 atcagcagga agcgagtctg ccgaagcgag ccgctgctcg aattcccaag ccgggtagaa
     3421 ttgcccgtca aagcggataa ggggcctgct gtgaagacgg cgcaagagct cgcgcct
//
LOCUS       DAOQJF010000348         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17321_length_3473_cov_4.982153, whole genome shotgun sequence.
ACCESSION   DAOQJF010000348 DAOQJF010000000
VERSION     DAOQJF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17321_length_3473_cov_4.982153"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="PLF85_14675"
     CDS             complement(<1..1486)
                     /locus_tag="PLF85_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE20966.1"
                     /translation="MELEQLPEIQKIIHIGKANGEITYDEINDILPEKLTNSDKIDDV
                     FILLNQYGIEIVEEYEKKQVGRRGDTSSAVVPGNFGSLGEQKVPKALVSELKAQGYEI
                     KQPENKGTLTIQDLAMQMLTRGYDLDALQLLLQKPEFRPAASKKKKSTGSAGASAADD
                     PIRLYLKEIGKISLISGDKEVELARRIEGGENIIEDAVLRSSLLRSAFIKAYNRVDKG
                     TLRITDIARASKTYYISTNEQKELREKFLREIKPVIDYDKQISSLKAKLKRYTHKSKK
                     HQEVWAQILKLEGLAADHVIRVGVSQKELQKHVNKIKSMVFRIKEIKRHFLKLKERYG
                     HDVKGIKAFNRYIERNEDLHIVERELGCTVEDVKNIIKDIRNNERKLRRMEQEAGSPT
                     LIILLWGEKISRGQREIDAAKKELINANLRLVVSIAKRFANRGMHFFDLIQEGNIGLM
                     RAVEKFEYRKGYKFSTYATWWIRQAITRALSEQARTIRIPSHMIE"
     gene            complement(1670..3352)
                     /locus_tag="PLF85_14680"
     CDS             complement(1670..3352)
                     /locus_tag="PLF85_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNE20967.1"
                     /translation="MNLKTYAEIRRLLTVSSIIVQFALDLLVSYLYRPRGLRKRSFSR
                     RMLAVAIRLLLRRPAGIIRFPVRLRQTIERLGPTYVKLGQILSLREDLLPRRITYELR
                     NLQTKVPPITYEEAKKVIEGEFNVPLRHIFKEFAPKPVGAASLAQAHIAYLRNGQKVV
                     VKVQRPGIIPVMTNDLRLMKRLAWILQQIPYIQDFQPQKLIQEFSDYTMKELDFTQEG
                     KHADIFRENFKDNDDVILPKVYWEYTTRKVLTLEFIEGVKPDDSEKLKKLGINGPKVA
                     ALGARVVIKQLFIDGFFHGDPHPGNLFIVGSEKFCMIDLGMIGQFSQKTMNAMFLYYY
                     YLIIRDYETASKYMVGLTETTAHSDIAGFRAEIEEIGKRWIGAGFKNYSLGKLILNSM
                     NMGARYKLYFNTDIMLAIKAIVTIEAVGFILDPKMDLAKVSLPIMSEIFVGRISPMRM
                     TKPILRALPDYLDFLEQAPATLLKTLGMVSTGKFQIEMVEKTEKQLPPEPAWKLWIPF
                     VALGLGIFTLSADNPPGGELSIGKNSHLPWVSAISFVIAAWYSIRFWRMRRV"
BASE COUNT          716 a          884 c          984 g          889 t
ORIGIN      
        1 gctcgatcat gtgcgaagga atacggattg tgcgcgcctg ctctgagagc gcgcgcgtga
       61 ttgcctggcg gatccaccag gtggcgtagg tcgagaattt gtagcccttg cggtattcaa
      121 acttctccac cgcgcgcatc agaccaatgt tgccttcctg gatcaggtcg aagaagtgca
      181 taccacggtt ggcgaagcgc tttgcgatcg agacaaccag gcgcaggttg gcattgatga
      241 gttctttttt cgcggcatcg atttcacgct ggccgcgcga aattttttcg ccccagagca
      301 gaatgatcag ggtaggtgag cccgcttcct gttccatacg gcgcagcttg cgctcgttgt
      361 tgcgtatatc tttgatgatg ttcttcacat cttcaaccgt gcagccgagc tcgcgctcaa
      421 cgatgtgcag gtcttcattg cgttcgatgt agcggttaaa cgccttgatg cctttcacat
      481 catggccgta gcgctctttc agcttgagaa agtggcgctt gatttccttg atacggaaaa
      541 ccatgctctt gatcttgttg acgtgtttct gcagttcttt ttgcgaaaca cccacgcgta
      601 tcacgtggtc tgcggccagg ccctcgagtt tcaggatctg tgcccagact tcctggtgct
      661 ttttactctt gtgcgtatag cgcttgagct ttgccttgag cgatgagatc tgcttgtcgt
      721 aatcgatgac gggtttgatc tcgcgcagga acttctcgcg cagttctttt tgttcgttgg
      781 tgctgatgta gtaggtcttt gaggcgcgcg caatgtcggt aatgcggagt gtacccttat
      841 caacgcggtt gtatgccttg ataaatgcgc tgcggagcag cgacgaacgc agaacggcgt
      901 cttcgatgat gttttctcca ccttcgatgc gccgcgcgag ttcaacttct ttgtcgccgg
      961 aaatcagcga tatcttgcca atttctttca ggtaaaggcg aatcgggtca tccgctgcgc
     1021 tggcgccggc agaaccggtg gatttctttt tcttcgaggc tgcaggcctg aattcaggct
     1081 tttgcagcag cagctgcagc gcatcgaggt cgtaaccccg ggtcagcatc tgcatcgcca
     1141 ggtcctggat tgtcagggtg cccttgtttt cgggctgctt aatttcataa ccctgcgctt
     1201 tgagttctga gaccagcgcc ttggggactt tttgttcgcc cagcgaacca aagttgccag
     1261 gtacaacagc cgacgacgta tcgccgcggc ggcctacctg cttcttttca tattcttcaa
     1321 cgatttcaat gccatactgg ttgagaagaa tgaagacgtc atcgatcttg tcggagttcg
     1381 tcagcttctc aggaaggatg tcgttgatct cgtcgtaagt aatttcgccg ttggctttgc
     1441 cgatatgaat aattttttgt atttcgggaa gttgttcgag ctccactaag actcctctgt
     1501 gctgtaagtg accgcagcct tcccttgagt tatcgggatt taccccgacc caagtttacc
     1561 actgacgtca tagttaattt atgggacacc gcgtaaagaa ggtgccaaaa acgcggccga
     1621 tagtccagtt ttccgggcat tttcatcttc tggtatcggc gatatccggt cagacccggc
     1681 gcatccgcca gaacctgatg gaataccagg cagcaataac aaagctgatc gccgaaaccc
     1741 aaggcaaatg ggagtttttt cctattgaga gctctccgcc agggggatta tctgccgaga
     1801 gggtaaagat acccaggcca agcgccacga agggtatcca gagcttccat gcgggctcgg
     1861 gtggcagctg cttctcggtc ttttcgacca tttcgatctg gaatttgcca gttgatacca
     1921 taccgagtgt cttgagcagc gtggcaggtg cctgttcgag aaagtccagg tagtcgggca
     1981 gggcgcgcag aattggcttg gtcatgcgca tcggcgaaat gcggccgaca aaaatctcgc
     2041 tcataatggg gagcgatact ttggccaggt ccattttcgg gtcgaggata aagcccaccg
     2101 cttcaatcgt cacgattgcc ttaatcgcga gcatgatatc cgtgttgaaa tagagcttat
     2161 agcgcgcgcc catgttcatc gagttcagaa tcagcttgcc cagcgagtag ttcttgaaac
     2221 cggcgccgat ccagcgttta ccgatttctt caatttcggc gcgaaagcct gcaatgtccg
     2281 aatgcgctgt ggtttcggta aggccgacca tgtacttcga tgcggtctcg tagtcgcgga
     2341 tgatcaggta atagtagtac aggaacattg cgttcatggt tttctgcgag aactggccga
     2401 tcatgcccag gtcgatcatg caaaatttct cagaaccaac aatgaacagg ttaccggggt
     2461 gcgggtcacc gtggaagaag ccgtcgatga agagctgttt gatcacgacg cgcgcgccga
     2521 gcgcggccac tttcggccca ttgatgccaa gtttcttgag tttttccgag tcgtcgggtt
     2581 taactccctc aataaactcg agggtgagta ccttgcgggt ggtgtattcc cagtagacct
     2641 tcgggagaat gacgtcgtcg ttgtccttga agttctcgcg gaagatgtcg gcgtgtttgc
     2701 cctcttgcgt gaagtcgagc tccttcattg tgtagtcaga gaattcctgg atcagcttct
     2761 gcggctggaa gtcctggata tagggaatct gctgcagaat ccacgccagg cgcttcatca
     2821 ggcgcaggtc gttcgtcatg accgggataa taccgggacg ctgcaccttg acgacgacct
     2881 tctggccgtt tctcaggtag gcgatatgcg cctgcgccag cgaagccgcg cccacaggtt
     2941 tgggtgcaaa ctccttgaag atatggcgga gtgggacgtt aaattcacct tcgattacct
     3001 tcttggcctc ttcgtaggta attggtggta ccttggtctg caggttacgc agctcgtagg
     3061 tgatacggcg gggcaagaga tcttcacgca gcgagaggat ctgcccgagt ttgacatagg
     3121 ttggcccgag gcgttcgatg gtctggcgca ggcgtacagg aaaacggata atacccgcag
     3181 ggcggcgtaa cagcagacga atcgccaccg caagcatgcg gcgcgaaaaa gaccgcttgc
     3241 gcagaccgcg ggggcggtag aggtacgaga ccagcaggtc gagcgcgaac tgcacaatga
     3301 tcgagctcac cgtcaacagg cggcgtattt cggcgtatgt cttgagattc atgcgtacaa
     3361 aggcacccgc aacgcagcgc gggaagcagt tggcagcatt ttagaaggga gcgtccgaga
     3421 caagtgtttt ccaacacata tctgctccgc tgtgccgctt tttgcggtgg gct
//
LOCUS       DAOQJF010000349         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17330_length_3472_cov_5.743635, whole genome shotgun sequence.
ACCESSION   DAOQJF010000349 DAOQJF010000000
VERSION     DAOQJF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17330_length_3472_cov_5.743635"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLF85_14685"
     CDS             <1..333
                     /locus_tag="PLF85_14685"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNE20968.1"
                     /translation="KSPSYALTTDCALDFCHMQLLSNDELVHAMQQLPDWHLHHGALA
                     KDFVFADFSQAFGFMTRVAMIAEKLDHHPDWNNTYNRVHISLMTHDKGGLTKRDAQMA
                     LEIEKLLA"
     gene            330..1313
                     /locus_tag="PLF85_14690"
     CDS             330..1313
                     /locus_tag="PLF85_14690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE20969.1"
                     /translation="MISVLLPCHREGHLLQRAIDSVLAQTLPDWQLVLVCNNTDETTL
                     AVAQAAAQNDRRIFLVHEHTPGIAYALNTGLKHCTGDYVARLDADDTMPPGRLAMQKV
                     YLDSHHETGLVSGQVRFVTDEGAGYDHYVQQINGWQTHEQMYRYRFVESPVAHPSVMF
                     RRSLIDRFGDYSTGNVPEDYELWLRWLQAGVRFGKTDEVVLDWYDSPGRLSRTHDNYA
                     RRAFDEVRLQYLARALPDLAHGRPLWVCGGRYARRKSVRLKSLGIQVTGIVDVIERRL
                     ETLGSMTYEQLPSPGSIFLVSMVSNRDGYRVVEEVLTQKGYVAERDYVLAA"
     gene            complement(1352..2662)
                     /locus_tag="PLF85_14695"
     CDS             complement(1352..2662)
                     /locus_tag="PLF85_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20970.1"
                     /translation="MWRAAGGEADQIDENQLFSEGETVTPVEKVTDDKVQESTDKESV
                     AISGAINSRNAYSMNREWLTGNNASTAQNNLNNQIQTNLLLEARARRSTKAVVNVVNN
                     VYAQGRNEQRTFQDTTTGAVTKVNETVYYDFRFTEAFVDTNIARAVYFRAGKQVLVWG
                     RGFFWNPSDVINAELRSATDAFQFREGTYGLRAHVPIGSSLNFYSFTGVGRSNRAEDA
                     VQAFKAEAAFKRAEVATGVIMGQRKVPTWLAEASTRFLTLDWRAEVTYAYGDNAYRID
                     PTAFNPLFPVTYQLREQHVFKGVLSFGRSFEVFDVADRLGINFEYFYNGAGYKDNVFE
                     QNPLVRAYFVQNGLYRPNFYGMHYAGLFVTYARFFVQDLTLGVNTLANLSDKTMIISQ
                     LLTYNPLFNLTLTWQNNIFTGRPNGEYTVSGLGWYTEFSARVAF"
     gene            complement(2820..>3472)
                     /locus_tag="PLF85_14700"
     CDS             complement(2820..>3472)
                     /locus_tag="PLF85_14700"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNE20971.1"
                     /translation="SDFSAKVALTQQKVGQGVKNYETLFFRRDEGDEFLMVTTAPQSE
                     KGNGYLRTGNNFWMYRQNTRTFQHINRDESINGTDTKAGDFERKKTTELYKPFVDANG
                     KEDITEEMLGNKKVWKFTVVAKVRDVTYPKQVYWVEQGTYLQLKVQSYSLSGTLMQTA
                     YFIKYTQIEGKYILLKGMFIDEFEKGNKTVLEISGVSLKKLDSKIFTKAYLENLSK"
BASE COUNT          724 a          989 c          957 g          802 t
ORIGIN      
        1 aaatctcctt catacgcatt gaccaccgat tgcgctctgg atttctgcca catgcagctt
       61 ttgagcaacg atgaactcgt ccacgccatg cagcaattgc ccgactggca cctgcaccac
      121 ggcgcgctgg ctaaggattt tgtctttgcc gactttagcc aggcctttgg tttcatgacg
      181 cgtgttgcga tgatcgccga aaagctcgac caccacccgg actggaacaa cacctacaat
      241 cgcgtgcaca tctcgctcat gacgcacgac aaaggcggcc tgacgaaaag agatgcacag
      301 atggcgctgg agattgagaa gctactggct tgatctcggt tcttcttccc tgccaccgcg
      361 aaggtcattt actgcagcga gccatcgatt cggtgctcgc gcagacgctg cccgactggc
      421 agttggtcct ggtctgcaat aacactgacg aaactacact ggctgtagcc caggcggcgg
      481 cgcagaatga caggcggatt ttcctcgttc acgaacacac acccggaatc gcttatgcac
      541 tcaacaccgg gctgaaacat tgcacaggcg actacgtcgc acggctcgat gccgatgaca
      601 ccatgccacc cgggcgactc gctatgcaga aagtttacct ggactcgcac cacgaaaccg
      661 gcctcgtcag cgggcaggtt cgctttgtga ccgatgaggg cgcgggctac gaccactatg
      721 tgcagcagat caatggctgg cagacgcatg aacagatgta ccgctaccgt tttgtcgaat
      781 cgccggttgc tcaccccagc gtgatgttcc gccgcagcct gatcgatcgc tttggcgact
      841 attcgacagg aaatgttccc gaagactatg aactctggct gcgctggctg caggcaggtg
      901 tgcgctttgg taagacggac gaggttgtac tggactggta tgactcaccg ggcaggcttt
      961 cccgcacgca cgacaactac gcccgccgcg cgttcgacga ggtgcgcctg caatacctcg
     1021 cccgcgcgct gcccgatctt gcacatggca ggccgctctg ggtgtgtggc gggcgttatg
     1081 cccgaagaaa atccgtccgt ctgaagagtc tcggcataca ggttacgggc atagtcgacg
     1141 tcatcgagcg ccggcttgaa acacttggct caatgaccta cgaacaattg ccttcgccag
     1201 gcagtatatt tttggtctcg atggtctcga accgcgatgg ctaccgggtt gtcgaagaag
     1261 tcctcaccca aaaggggtat gtggctgaac gggactacgt gctcgcagcc taagttaccg
     1321 gaacctgcct ttgggcgctt tgcgcaacct gctagaaagc gacgcgcgca gaaaattcgg
     1381 tataccagcc gagaccgctg acggtgtatt cgccgttggg cctgccggtg aagatgttat
     1441 tctgccaggt aagcgtcagg ttgaagagcg ggttgtaggt cagaagctgt gaaatgatca
     1501 tcgtcttgtc gctcaggttc gccagggtat ttacgccgag cgtaaggtcc tggacaaaga
     1561 agcgcgcgta ggtgacgaac aaccccgcat agtgcatgcc gtagaaattg ggccggtaga
     1621 ggccgttctg gacgaagtag gcgcgcacga gggggttctg ctcaaacaca ttgtctttgt
     1681 aaccggcgcc gttgtagaaa tactcgaagt tgatgcccag ccggtcggcg acgtcgaaca
     1741 cttcgaagct ccgcccaaaa ctcaaaacgc ctttgaacac gtgctgttcg cgtaactggt
     1801 aagtcaccgg aaaaagcggg ttaaatgccg tcgggtcgat acggtaggca ttgtcgccat
     1861 aggcataggt cacctcggca cgccaatcga gagtcaaaaa gcgcgtactc gcctcggcaa
     1921 gccaggtcgg caccttgcgc tggcccatga tgacaccggt cgccacttcg gcgcgtttga
     1981 acgctgcctc agctttaaat gcctgcactg catcttcagc ccggttgctg cggccgacgc
     2041 cggtgaacga atagaagttc aggctgctac caatcggcac gtgcgcgcgc aggccgtacg
     2101 tcccttcgcg aaactggaag gcgtcagtcg cactcctgag ctccgcattg atcacgtcgg
     2161 aagggttcca gaaaaaaccg cgcccccaga cgagcacctg cttgcccgcg cgaaaataca
     2221 ccgcgcgcgc aatgttggta tcgacaaagg cttcggtgaa gcggaagtcg tagtagactg
     2281 tttcgttcac tttcgtgaca gcgcccgtcg ttgtatcctg gaacgtacgc tgttcgttgc
     2341 gcccctgcgc gtagacgtta ttcaccacgt taacgacggc cttggtactg cggcgggctc
     2401 tcgcctcgag cagcaggttc gtctgtatct ggttgttgag gttattctgc gcggtcgatg
     2461 cgttgttgcc agtcagccac tcgcggttca tcgagtaagc attacggctg ttgattgcgc
     2521 ccgatatcgc caccgactct ttgtcggtag attcctgcac tttgtcgtcg gtgaccttct
     2581 caacgggtgt aaccgtttca ccttcggaga aaagctgatt ttcgtctatt tggtctgcct
     2641 cacccccagc cgctctccac atagtggaga ggggagccaa accggttctc gcccttttgg
     2701 aaagctgatc tttctccccc tctccatatc catggagagg gggccggggg gagaggttag
     2761 cctgcgcgaa aattgcacca accagaatgc atccagctat gacgaaaaca atgcgcacgc
     2821 tatttactca ggttctcgag gtatgccttg gtgaaaatct ttgaatcgag tttcttcagc
     2881 gacacacccg aaatttcgag caccgtctta ttgccttttt caaactcgtc gataaacata
     2941 cctttcagta agatgtactt gccctcgatc tgcgtgtact tgataaagta agccgtctgc
     3001 atcagtgttc ccgacagcga gtaggattgt accttgagct gcaggtaggt accctgctcg
     3061 acccagtaga cctgttttgg gtatgtcacg tcgcgcacct tggcgacgac ggtgaatttc
     3121 cacaccttct tgttgcccag catctcttcg gtgatgtctt ctttgccgtt ggcgtcgacg
     3181 aagggcttat acagttcggt tgtcttcttg cgctcgaaat ctcccgcctt ggtatcggtg
     3241 ccgttgatgc tctcatcgcg gttgatgtgc tggaacgtgc gggtattctg ccggtacatc
     3301 cagaagttgt tgccggtacg caggtaacca ttgcctttct ctgactgcgg cgccgtcgtc
     3361 accatcagga attcgtcgcc ttcgtcgcgg cgaaaaaaca gcgtttcgta atttttcacg
     3421 ccctgtccaa ccttctgctg cgtcaatgcg accttggcag aaaaatcgct ga
//
LOCUS       DAOQJF010000350         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17355_length_3469_cov_5.240187, whole genome shotgun sequence.
ACCESSION   DAOQJF010000350 DAOQJF010000000
VERSION     DAOQJF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17355_length_3469_cov_5.240187"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PLF85_14705"
     CDS             <1..903
                     /locus_tag="PLF85_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNE20972.1"
                     /translation="STDGVAFDSGRVATTNSATFSNVAAGKVYHFTVQAGNASGLSLA
                     SEVVSIRVPVNATDKKLLIVNGFDRLDNRVNFVTNYDKRAVGPQVERGNTFNYTPVYL
                     KAVVGSTRPWSVSSASNEAVSRGRVNLSGFDAVVWYTGRESWSDETFSYSEQQLVTSY
                     LNAGGRMIITGSEIGWDLAAQTIGNTHANDLTFLESLLRTRYAGDDAGTSTLNAGSGV
                     LSGVAGFTLDDGLSGSYQNLFPDYYTAVNGSTCVQQYNTASRCAILHYTGTYRLFSLG
                     FPFEIITADAARNTLMEKMLTALE"
     gene            969..1574
                     /locus_tag="PLF85_14710"
     CDS             969..1574
                     /locus_tag="PLF85_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20973.1"
                     /translation="MVTWLADSPGKEPRAIRPEPRPLDDYRRQFRRPDGSLLSDAELR
                     QVLQAKGIPYQETCPDGPNCSSGAATAVLAIPQVDFSTAGITAPRRDELRVAIATAVG
                     GQLEAMRRINPELRLERIAVDSGMKLQLHFNKQFEQIADDETKLNEFSEGLHSLGVAG
                     LRGSVVYIEGQPLGLYLQKKDAERNQKARAESPENGGDNRR"
     gene            complement(1587..3134)
                     /locus_tag="PLF85_14715"
     CDS             complement(1587..3134)
                     /locus_tag="PLF85_14715"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNE20974.1"
                     /translation="MRIAIPLFCILGALSALQAGGATAETFDIAGFRKAAEKNIRKKT
                     LPNGMRVILMRQPSSPTIACYLKIGVGSANESFDQAGTAHFLEHLLFKGTPTMGTTNY
                     PEEKKLLEKIESTGERLDSLERALLNPLITDKERSNLKKRMAEQEKLLASLQAEAQAF
                     VISEEDSQAYSLAGQVGYNAYTTTDVTNYQIKLPANRLAMWAELESNRFLNPVLREFY
                     PERKVIQEERRMRYDSRPTSLLYELFLKTAFGMSPYGKPVIGFEKSMHRLRLRETRQF
                     FERNYIPSRMVITVVGELEFDEAFATIEKYFGRLKARPTPEFPPTVYEPQRGRRIAWL
                     EAKNSPTMITGWARPPAMHDDDKALEVLSRLLGDGQTSRLVNRLVIQDKLAGDVKVGS
                     STPGEKLDTQFTIFADVFDAASYPAIEKAIAEELERLRTNGPTAEELQKIKNRYIAEI
                     VQTLSQNAGMADTLSYYELLLNDYQKLFSSLEEINEVDAAKIKAVVAKYFTDGRNTTV
                     YLKPLQK"
     gene            3247..>3469
                     /locus_tag="PLF85_14720"
     CDS             3247..>3469
                     /locus_tag="PLF85_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNE20975.1"
                     /translation="MEKAKLPEFIETKYEEKNNNPAVLEDTEVRRAVVTVIDMLDRGE
                     LRVATPPQTGDNQTDQWRVNAWVKKAILMY"
BASE COUNT          728 a          933 c         1013 g          795 t
ORIGIN      
        1 tcgaccgatg gtgttgcgtt cgattcaggg cgggtggcga caacgaactc cgccacattc
       61 agcaatgtcg ccgcgggcaa ggtttaccat ttcaccgtgc aggcaggcaa tgccagcggt
      121 ttgagcctcg catccgaggt ggtgagcatt cgcgtgccgg tgaatgcgac agacaagaag
      181 ctgctcatcg tcaacggctt tgaccggctg gataaccgcg tgaacttcgt gactaactac
      241 gacaagcgtg cggtcgggcc acaggtagag cgcggcaata ctttcaacta cacacccgtc
      301 tacctgaagg cagtggttgg cagtacgcgg ccctggtcgg tttcgagcgc ctcgaacgaa
      361 gcggtttcgc ggggcagggt aaacctgtca ggtttcgatg cagtggtctg gtatacgggg
      421 cgcgaaagct ggtcagacga aaccttcagc tatagcgaac agcagctggt gaccagctat
      481 ttgaacgctg gcggccgcat gataattacc ggttcggaga ttggctggga tctcgctgcg
      541 caaacgatcg gcaacaccca cgcgaatgac ctcacatttc ttgaaagcct gctgcgtacc
      601 cgttacgcgg gcgacgacgc cggcacgtcg acactcaatg cgggcagcgg agttctctct
      661 ggggtcgcgg gttttaccct cgacgacggg ctttcgggaa gttaccagaa tcttttccct
      721 gattattaca ccgccgtgaa cgggtcaacc tgcgtgcagc agtacaatac agcctcgcgc
      781 tgcgcgattc tgcactatac cggtacttac cggctattct ctctgggttt tccgtttgag
      841 attatcacgg ccgatgccgc acgcaatacg ctgatggaaa agatgcttac ggcactcgag
      901 tagggggagc gttgcttcgc agacgattcc tgtatgtctc gctactagtc ctctctgtta
      961 ctgcattttt ggtaacctgg ctggctgatt ctccgggaaa agagccccgc gccatacgcc
     1021 cggaaccaag accccttgat gactaccgta ggcagtttcg tcgccctgat ggctcgctac
     1081 tttccgatgc cgagttacgc caggtgctgc aggcaaaagg tattccgtac caggagacct
     1141 gtcccgatgg gccgaactgc agcagtggag cggccacggc tgtcctggcg attccgcaag
     1201 tcgacttttc cacagcgggc attacggccc cgcggcgtga cgagctgcgt gtggcgattg
     1261 cgacagccgt gggcggccag ctcgaagcca tgcgccgtat taaccctgag ctccgcctcg
     1321 aacgaatcgc ggtcgattct ggcatgaagc tgcagctgca tttcaacaag cagtttgagc
     1381 aaatcgccga tgacgagacg aagctgaacg aattctccga ggggttacac agcctgggcg
     1441 tggctggttt gcgcggttcg gtcgtgtaca tagaggggca gcccctgggg ttatacctgc
     1501 agaagaaaga cgctgagcga aaccagaagg cgcgcgccga atcgccggaa aacggcgggg
     1561 ataaccggcg ctgatcttta ccctgcttac ttttgcagcg gtttgagata cacggtcgtg
     1621 ttccgtccgt cggtaaaata ctttgccacg actgctttta tctttgcggc atcgacttcg
     1681 ttaatttctt cgagcgagct aaaaagtttc tggtagtcgt tcagcagcag ttcgtagtaa
     1741 gaaagtgtat ctgccatacc ggcgttctgg ctgagagtct ggacgatctc tgcaatgtag
     1801 cggttcttga ttttctgcaa ttcttctgcg gttggcccgt tcgtgcgcag gcgttcgagc
     1861 tcttcggcga tggccttttc aatcgcggga taactggccg catcaaacac gtcagcaaaa
     1921 atcgtgaact gtgtatcgag tttttcgccg ggtgtcgatg aaccgacctt cacatcgcct
     1981 gcgagtttat cctggatgac gagccggttg accagccgcg aggtttgccc gtcaccgagc
     2041 aggcgtgaca gcacctcgag cgctttgtcg tcgtcgtgca ttgccggcgg ccgcgcccag
     2101 ccggttatca tagtggggga gtttttcgcc tcaagccagg cgatacggcg gccgcgctgc
     2161 ggttcataga cggtcggcgg gaattcaggc gtcggccgcg ccttgaggcg accaaagtat
     2221 ttttcaatcg tcgcaaaagc ttcatcgaat tcgagctccc cgacaacagt aatgaccatg
     2281 cgcgacggta tgtagttgcg ctcaaaaaac tggcgggttt cgcgcaggcg caagcggtgc
     2341 atcgacttct caaaaccgat cacgggcttg ccataggggc tcatgccgaa agcggtcttg
     2401 agaaaaagtt cgtaaagcag cgaggtaggg cgtgaatcat agcgcatccg ccgttcttcc
     2461 tggatgactt tgcgctcggg gtagaattcg cgcagtaccg gattcaaaaa ccggttgctc
     2521 tccagttcag cccacatcgc gaggcggttt gccggcaatt tgatctggta gttcgtgacg
     2581 tcggtcgttg tgtacgcatt gtaccccacc tggcccgcga gcgaataggc ctgcgagtct
     2641 tcttcggaaa tgacaaatgc ctgcgcctct gcctgcaggc tcgcgaggag tttttcctgt
     2701 tcggccatgc gctttttcag gttgcttctt tctttgtccg tgatgagtgg attcagcagc
     2761 gcgcgctcga ggctatcgag ccgctcgccg gtggactcaa ttttttccag cagcttcttt
     2821 tcttccgggt agttggttgt gcccatcgtc ggggtgcctt tgaaaagcag gtgctcgaga
     2881 aagtgtgcag tacccgcctg gtcaaaggac tcattggccg agccgacgcc aatcttcagg
     2941 tagcaggcaa tggtagggct cgaaggctgg cgcatcagga taacgcgcat gccattaggc
     3001 agagtttttt tccggatatt tttttcggct gctttgcgaa agccggcaat atcaaacgtc
     3061 tctgcggtag ctccgcctgc ctgcaaagca gacaaagcgc caagaataca gaagagcggg
     3121 attgcaatac gcattaacca gaattgtgag ccaaattaac ctgaccagcg tatagctatt
     3181 gttcagcctt gccggtaggt tcaggatttt actttgcacg atgtttactg attggggtct
     3241 atttgcatgg aaaaggccaa gctccccgaa tttatcgaaa ccaagtacga agaaaagaac
     3301 aacaacccgg cggttctcga agatacagaa gttcgccggg cagtggtcac cgtgattgac
     3361 atgctcgacc ggggcgagct gcgcgtggca acgccgccgc agacgggcga taaccaaacc
     3421 gaccagtggc gggtgaacgc ctgggtaaag aaagcaatcc tcatgtatt
//
LOCUS       DAOQJF010000351         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17404_length_3463_cov_4.742664, whole genome shotgun sequence.
ACCESSION   DAOQJF010000351 DAOQJF010000000
VERSION     DAOQJF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17404_length_3463_cov_4.742664"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1148)
                     /locus_tag="PLF85_14725"
     CDS             complement(204..1148)
                     /locus_tag="PLF85_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE20976.1"
                     /translation="MFRLATLLLVAALALATGTLTAADLSPNELGKIPVLTYHKIAGE
                     DTEYTRSRQGFASDLAQLKKHGFYPMTLAEMRSGKITTPKGKIPVLITFDDSSESQFK
                     YEADGSLTPGCGVEIMERFQKQNTDFPLRGVFFVLPKAQYPSNFFGQTDKIGKKLQYL
                     TGKGFEIGSHTLWHANLKKYRNKIEEQLALSVAEIHKYLKDYPVHAFALPYGIFPPKE
                     DEIRLRSGSFKGKSYRHDMIFDYSNGLSLSVYDQKFNSYRIRRLHGNEKAMQKLFRQV
                     VKEKGIFFISDGNAETVTVPKSEAGRVKLRPGQKLVTY"
     gene            complement(1352..2281)
                     /locus_tag="PLF85_14730"
     CDS             complement(1352..2281)
                     /locus_tag="PLF85_14730"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE20977.1"
                     /translation="MRNLIISTAVVALVAVGCGEKKEQASLDSAKFTYVKGDVLVSGK
                     PATLGQTVSKDATIEVKNNSMAVLQFSSAASITLKANSVLSIANLAKNDAGKPVIELS
                     QSNGSSFSKIAKGQSDFSIKTPTAVAGVRGTSFELEVGNGKTTKIKLLEGKVAVAKVE
                     ANDTPEEVKAELEKATVVEAGQKVEVDNKAVSKPVEITEAEKTSLQAMNTVEIAPVSG
                     KVVVPESVEKYATGETTSASLTEAAPKAAEVKKLTLADIKAKYGRIAKIQTKNGKEYV
                     GYFNQQGAEMTIQTVDGQVRVPVASVQKVTPMN"
     gene            2341..3342
                     /locus_tag="PLF85_14735"
     CDS             2341..3342
                     /locus_tag="PLF85_14735"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNE20978.1"
                     /translation="MSIKKFNFLANFSSLNSWGAEGVPFVALVSFDGQLLAAPLSQAD
                     WHAGQVDFPGGRLHFRVYGAAQDDDAGMDSHGKPAFSILALPPRQSVIAAISALQSEM
                     RAGHSYLVNYCSQTSVATHYSPAELFAAAKAPYTFWLEGQFLSFSPEPFVTVRGREIS
                     TTPMKGTGTDKAALLADAKEQAEHATVVDLLRNDLGQVAGHVRVEDYRYVSEIQRGDG
                     RVLYQTSSRICGEMPADWRSHIGDWLPRLLPAGSISGAPKKETLELIRRFEAEPRGFF
                     TGVAVLFDGENLSSAVLIRFLDLVGDVLKFRSGAGITIYSDPAQEYDEIVSKVYFPA"
BASE COUNT          714 a          897 c          937 g          915 t
ORIGIN      
        1 gaaaaagcct ggttgagttt caggctggct tcacgctcgg ctttttcatc gagggcgggc
       61 agatcggtga aaaaagaaag cgccaaaagg cagaagagta ggcgattaaa cattgcttgg
      121 ggtttgctcg ctcggctaat cttagcggca agctttttct ctgccttcag ggctggcctg
      181 tgcgggagtt tttccgaaac ctgctagtaa gtcacaagtt tctgtccagg gcgcagtttt
      241 acccggccgg cttccgattt cgggacagtc accgtctcgg cattgccgtc gctgatgaag
      301 aaaatccctt tttctttcac gacctggcgg aacagcttct gcatcgcctt ttcattgccg
      361 tgcaggcggc gtatgcggta agagttgaat ttctggtcgt ataccgacag gctcaggccg
      421 ttcgaataat cgaagatcat gtcgtggcgg tagctcttgc ctttgaagct gcccgaacgc
      481 aggcggattt catcttcttt cggcgggaaa atgccgtagg gcagggcaaa agcatgcacc
      541 ggatagtctt tcaggtattt atgaatttcc gccactgaaa gggccagctg ttcttcaatc
      601 ttgttgcggt attttttcag gtttgcatgc cacagggtgt gcgagccgat ctcaaagccc
      661 ttgcccgtca ggtactgcag tttcttgccg atcttgtcgg tctgaccgaa gaaattgctc
      721 gggtactgag ccttgggcag aacaaaaaac actccgcgta gcggaaaatc ggtattctgc
      781 ttctggaatc gctccataat ctctacaccg cagccgggtg tgagtgagcc gtcggcttcg
      841 tatttaaact ggctttcgga tgaatcgtcg aaagtgatca gcacggggat cttgcccttc
      901 ggcgtggtaa tctttccgct gcgcatttcg gccagcgtca tcgggtaaaa accatgtttc
      961 ttgagctgcg cgaggtcgct cgcgaaaccc tgccggctgc gcgtgtactc ggtatcttcg
     1021 ccagcaatct tgtggtaggt gagaacagga attttgccca gttcattcgg cgacaaatcg
     1081 gcggcagtta aagtgccggt cgcgagcgcg agggctgcga caagtaacaa ggtagcgagg
     1141 cgaaacatag gccagttttg gaaaacaaga cagggtgaaa tgcgtgtttg gttgcccacc
     1201 gggcataaaa aaacccgctc actctcgtgg gcgggttgtt agttcatgca ccgcagctcg
     1261 gttagctgcg gtgccctgac taatcgcttt cggcgtccgc gaccccgtgg ccgcagaccc
     1321 taacgttggc agcttgaggc tgccaacccg atcaattcat aggtgtaact ttctgaacgc
     1381 ttgcaactgg tacgcgaacc tggccatcca cagtctggat agtcatttca gcgccctgct
     1441 ggttgaagta accgacgtat tccttaccgt ttttggtctg gatcttggcg atacggccgt
     1501 atttagcctt gatatctgcc agagtcagtt tctttacttc agccgctttt ggtgcagctt
     1561 ctgtcagtga agcagaagtt gtttcgcctg ttgcgtattt ttcaacagac tcgggaacta
     1621 ctaccttgcc gctcactgga gcgatttcga cagtgttcat agcctgcagg ctggtctttt
     1681 cagcttcagt gatttcaact ggctttgata cagccttgtt gtccacttct actttctggc
     1741 cagcttcaac aacggtagct ttttcgagtt cagccttcac ttcttcaggc gtatcgtttg
     1801 cttctacctt tgctacagca actttacctt cgaggagctt gattttggta gtttttccgt
     1861 taccaacttc gagctcgaac gatgtaccgc gcacacccgc gacagcggtt ggcgttttga
     1921 ttgaaaagtc tgactggcct ttcgcgatct tgctgaaaga tgaaccatta gactgtgaca
     1981 gttcgatcac aggcttgccg gcatcgttct ttgcgaggtt agcgatggac agtacagagt
     2041 tagccttcag agtgattgaa gcagcgctgg aaaactgcag aactgccatt gagttgtttt
     2101 tcacttcaat agtagcatct ttgctcacgg tttggccgag agttgcaggt ttgccgctta
     2161 ccagtacgtc acctttgaca taggtaaact ttgctgagtc aagactcgct tgttcttttt
     2221 tctcgccgca accaactgcc accagggcaa ccactgcggt agagatgatg agattacgca
     2281 tttataatcc tcctaccaga ttctttacgt ctcactgtta ccttacagct tcgaccacaa
     2341 gtgtccataa aaaaattcaa ttttttggcg aatttttctt ccttaaatag ctggggggca
     2401 gagggagtac cctttgtggc actggtgagt tttgacgggc agcttcttgc cgcacccctt
     2461 tctcaggcag attggcacgc cgggcaggtt gattttccag ggggacggct gcattttcgg
     2521 gtttatggtg cagcgcagga tgacgatgca ggtatggatt cccatggcaa gccagctttt
     2581 tcgatccttg cattgccacc aaggcagagc gtcatcgctg ccatttcggc cttacagtca
     2641 gaaatgcgcg ctggtcattc ttacttggtg aactattgtt cacaaacatc ggtggcgact
     2701 cactattcac ctgcagaact ctttgccgcc gccaaagctc cctatacttt ctggcttgag
     2761 ggacagtttc tctccttttc acccgagccc tttgtcacgg tgcggggccg cgagatcagc
     2821 acgaccccga tgaagggcac cggcacggat aaggctgcgc tgttggccga cgccaaagag
     2881 caggccgagc acgcaactgt cgtcgatctg ttacgcaacg acctgggcca ggtggcgggc
     2941 catgtccggg tcgaagacta ccgctacgtc agtgaaatcc agcgcggcga tggccgcgtg
     3001 ctttaccaga cgagttcgcg catctgcggt gaaatgcccg ccgactggcg ttcgcacatc
     3061 ggtgactggc tgccgcggct gctgccggcg gggtcgatca gcggggcacc caaaaaagaa
     3121 accctcgaac tgatacgccg ctttgaggca gaaccaaggg gattttttac gggggtagcg
     3181 gttctgttcg atggcgagaa cctttcgagt gccgtgctga ttcgttttct cgatctggtg
     3241 ggcgacgtgt tgaagtttcg cagcggcgcc ggcatcacga tctacagcga cccggcgcag
     3301 gaatacgacg aaattgtcag caaggtgtat tttcccgcat gatgcgtttt attgaatcga
     3361 tctacctggc cgacggcgag ttgcgcaatc tcggactaca ccagcgccgt ttgcagctaa
     3421 cgcaactcgc gcactttggt tttactgcgc gcatcgatct gaa
//
LOCUS       DAOQJF010000352         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17580_length_3442_cov_4.454384, whole genome shotgun sequence.
ACCESSION   DAOQJF010000352 DAOQJF010000000
VERSION     DAOQJF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17580_length_3442_cov_4.454384"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLF85_14740"
     CDS             <1..331
                     /locus_tag="PLF85_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE20979.1"
                     /translation="ARFCVLYSPFRPICPPIMQEVLQGFRSDTDYNAARFFLDDMEKL
                     EAEPYHAAHGATEIYRSLRKRGLTIRKSTDCLIAWYALQARIPVLHRDRDFDMMAKPL
                     KLKILKI"
     gene            328..762
                     /locus_tag="PLF85_14745"
     CDS             328..762
                     /locus_tag="PLF85_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20980.1"
                     /translation="MNRFAYWVSAIFNPLFVGIPVMLAIGIAETGIRWEATPTVLAAI
                     GRLVVATLAFGLPNVILIALISLRLKVSLHCAGIGGMLPAVAYPFGMPGLTAGTAALL
                     LTAWSRVRMNEHSTIEVITGSILGTGLKALELAWLFGWPRVI"
     gene            898..1404
                     /locus_tag="PLF85_14750"
     CDS             898..1404
                     /locus_tag="PLF85_14750"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE20981.1"
                     /translation="MALPSPRELLRKAGRGLIYLAGFFIFAAVVNYFQSPGTGNLAPL
                     QSLPLTSMSGEKLQIPVASGKKTVIYFFAPWCAVCKVSMDALNFFEGSDRVQTLAVGL
                     DYDNIAEVKPFQDKLATPIYAGDLQLQRQFKVDRYPTAYILNGDGSVAHVMVGYTSRF
                     GIWIRTLL"
     gene            complement(1417..1854)
                     /locus_tag="PLF85_14755"
     CDS             complement(1417..1854)
                     /locus_tag="PLF85_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE20982.1"
                     /translation="MPTQFLAGVVAIVWRADTDSYLLVRRSPTKGYAAGKWWVVTGTV
                     ESNEGFPGALVREVREELGAEVAQWRLLETTHFNRDGAEWLSAAFLCILVDHDRLVLD
                     DEHDAFEWVKASEVEQRVVNEEYPTDWITSALRLAEQHKAEFC"
     gene            complement(1865..2707)
                     /locus_tag="PLF85_14760"
     CDS             complement(1865..2707)
                     /locus_tag="PLF85_14760"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNE20983.1"
                     /translation="MLVRSDHRTVRLISGEREQSVWRKDQPDYLVHSYAQLVALAALT
                     WPGFVAKKPGRTLIAGLGGGVLCRWFMRHFAGMQVDVVEPDRNVIEIAREYFDLDKRV
                     CVFERDGRSHFEKHNAKYDIIMLDAFDNTYIPADMLSIEFLRLVQKRLQPGGVLIANG
                     WVSADFNLHEDATYVAAFGSVWELRRSPNIDGNRIIVCNEDLKHMEDLRPLLRERALL
                     LDERLGHPTQNPIPGARRMLSYLEMTNRLKLTKMTESPAAIILTDGNADRMRKRATFD
                     YATD"
     gene            complement(2677..2889)
                     /locus_tag="PLF85_14765"
     CDS             complement(2677..2889)
                     /locus_tag="PLF85_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20984.1"
                     /translation="MATRQPFVLFGVQGRFRRGLWHSVSELRARDAQHLEKYVLLDAR
                     EIKKTFCALCEMFSDIPCLFAQTTAP"
     gene            2984..3409
                     /locus_tag="PLF85_14770"
     CDS             2984..3409
                     /locus_tag="PLF85_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20985.1"
                     /translation="MLGLILGSIPSEVFDDAKEKLDRFCLEATQRHAANGNTSYRLLW
                     RRRGNAIVLSEEFATPAGKTVRYDSGKLEYESGLWRLFHLDVTQRWRRYLIAPATADF
                     ALAFSHWQRNETGIFSRADLRTNAKPAPRLQGKSVLVTN"
BASE COUNT          722 a         1032 c          935 g          753 t
ORIGIN      
        1 ggccaggttc tgcgtgttgt attcgccgtt caggccgatt tgcccgccga tcatgcaaga
       61 ggtactgcag ggctttcgtt ctgataccga ctacaatgcc gcgcggtttt tcctcgatga
      121 tatggaaaaa ttggaagccg agccatacca tgccgcccat ggggcaaccg agatctaccg
      181 ctctttgcgc aagcgcggtc tcactatccg caagagcact gactgcctga tagcctggta
      241 tgccctgcag gcacgaatac ctgttttgca tcgtgatcgc gattttgata tgatggccaa
      301 accgctgaag ctgaagatac tgaaaatatg aaccgcttcg cctactgggt ctctgccatc
      361 ttcaacccgc tcttcgtcgg catacccgtg atgctcgcaa tcggcatcgc cgaaaccggt
      421 atccggtggg aggccacgcc cacggtgctc gcggcgattg gtcgcctcgt cgtcgccaca
      481 ctcgcgttcg gtctgcccaa cgtgattctg atcgcgctga tttctctgcg cctcaaggta
      541 agcctgcact gcgcaggcat cggcggtatg ctgccggcgg ttgcttaccc gttcggcatg
      601 cccggactca ccgcaggcac ggctgcatta ctgctcacgg catggagccg cgtgcgcatg
      661 aacgaacaca gcaccatcga agtgattacc ggttcgattt tgggtacagg tctcaaggcg
      721 cttgaactcg cgtggctctt tggctggcca cgggttatct gaccggttgg tctttttggt
      781 tctgctgcgg cgctgcattc acccacttca cggccgacgc attcgccgcg cgcgcccact
      841 tgtaataacc acccaaagaa tatggacagc tgctggtttc gtgcatatca tatacccatg
      901 gccctacctt ctccacgcga gctgctgaga aaggcaggcc gtggtctcat ctacctggct
      961 gggtttttta ttttcgcggc cgtcgtgaac tacttccagt cgcccggcac cggcaatctt
     1021 gcgcctctgc aatcattgcc gctgaccagc atgtccggag aaaagctgca gatccctgtc
     1081 gcttcaggca agaagaccgt catctatttc ttcgcaccgt ggtgtgccgt ctgcaaagtc
     1141 agtatggacg cgctgaattt ttttgaaggc agcgatcgcg tgcagacact cgccgtgggt
     1201 ctcgactacg acaacatcgc tgaagtcaaa cctttccagg acaaactcgc cacaccgatc
     1261 tacgccggcg atctgcagct acagcggcag ttcaaagtcg accgctaccc cacggcatac
     1321 atcctcaacg gcgatggcag tgttgcgcac gtaatggtgg gttacaccag ccgcttcggc
     1381 atctggatca gaaccctgct ctaaccgctt gccccattag cagaattcag ctttgtgctg
     1441 ctcggcaagg cgcagcgccg atgtgatcca gtcagtggga tattcttcgt tcacaaccct
     1501 ctgctctact tcgctggctt tcacccactc gaaggcatcg tgctcgtcgt cgagcaccag
     1561 cctgtcgtga tctaccagga tgcataaaaa agctgcgctc agccattctg cgccgtcgcg
     1621 gttaaagtgt gtggtctcga gcaggcgcca ttgggccacc tcggcaccga gttcttcacg
     1681 gacctcgcgc accagcgcac cgggaaaacc ttcgttactc tctaccgtac cggtgacgac
     1741 ccaccacttg cccgctgcat aacctttcgt cggtgaacgc cgcaccagca ggtaagaatc
     1801 ggtatctgcc cgccagacaa tagccactac ccctgcaaga aactgagtcg gcatggtttg
     1861 tttgttaatc cgtcgcgtag tcaaacgttg cgcgcttgcg catgcgatcg gcattgccgt
     1921 cggtcagtat gatcgccgct ggcgattcag tcattttcgt caatttgagt cgattcgtca
     1981 tctcgaggta agagagcatg cggcgcgcac cgggaatcgg gttctgcgtc gggtgcccga
     2041 gtctctcgtc gagcagcagg gcacgttcgc gcagcagggg acgcaggtct tccatgtgtt
     2101 tcagatcttc gttacagaca atgatccggt taccgtcaat gttgggagaa cgcctgagct
     2161 cccacacgct gccgaacgct gcgacatagg ttgcatcttc gtgcaggtta aaatctgccg
     2221 agacccagcc attcgcgatc aacacgccgc ccggctgcag gcgtttctgc acgagccgca
     2281 gaaactcgat cgaaagcatg tctgcaggaa tgtatgtatt atcgaaagcg tcgagcatga
     2341 tgatgtcgta tttggcgttg tgcttctcaa aatggctgcg gccatcgcgc tcaaagacgc
     2401 acacgcgttt atcgagatca aaatattcac gcgcgatctc gatcacgttg cgatcgggtt
     2461 caacgacatc gacctgcatg ccggcaaagt ggcgcatgaa ccagcggcag agcacaccac
     2521 cgccaagacc agcgatgagc gtacgccccg gttttttcgc gacaaaccca ggccaggtga
     2581 gagcggccag ggccacgagc tgcgcgtacg aatgcaccag gtaatcgggt tggtctttgc
     2641 gccagacaga ttgttcgcgc tcacccgaga tgagtcttac ggtgcggtgg tctgagcgaa
     2701 caagcacggg atgtcagaaa acatttcgca gagtgcgcag aaagtttttt tgatctcgcg
     2761 tgcatcgagc agtacatact tttccagatg ctgcgcatcg cgggcgcgca gttcactgac
     2821 cgaatgccac aacccgcgcc tgaagcggcc ctgtactcca aagagtacaa agggttgacg
     2881 cgttgccact gctgcgtcgg gctgtgcctg agaatacgag caaagcgagg ttaccgatca
     2941 tgtctaccta tcaaaaaaca caccttatag gagtattttc ccgatgctcg gtctgattct
     3001 gggttcgatt ccgagcgaag tttttgatga cgcgaaagag aagctcgatc gcttctgcct
     3061 cgaggcgacg caacgccacg cagcaaacgg caataccagc taccggctgc tctggcggcg
     3121 gcggggcaac gcgattgtac tctctgaaga attcgccaca ccggccggaa aaaccgtgcg
     3181 ctacgattca ggcaaactcg aatacgaaag tggcctgtgg cggctgttcc acctcgacgt
     3241 gacgcagcgc tggcgccgct acctcattgc gccggctacg gcagatttcg cgctcgcctt
     3301 cagccactgg caaagaaacg aaaccggaat cttctcgcgg gctgacctgc gcaccaacgc
     3361 gaagccagcg ccgcgcctgc agggtaagtc agtactcgta accaattgag aagacaccct
     3421 gccagtcgcc gatcggtcgc ga
//
LOCUS       DAOQJF010000353         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17656_length_3433_cov_5.214328, whole genome shotgun sequence.
ACCESSION   DAOQJF010000353 DAOQJF010000000
VERSION     DAOQJF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17656_length_3433_cov_5.214328"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLF85_14775"
     CDS             complement(<1..514)
                     /locus_tag="PLF85_14775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HNE20986.1"
                     /translation="MLPLSILDLAPITQGSNAAQSFGHSLDLAQHAERWGYRRFWLAE
                     HHGMPGVASAATAVLIGHIAGGTKTIRVGAGGIMLPNHSPLVIAEQFGTLESLYPGRI
                     DLGLGRAPGTDQLTAQALRRNAHLAADNFPHDVLELLNLFSEKPEGKVRAVPGQGLQV
                     PVWILGSSLYG"
     gene            complement(595..667)
                     /locus_tag="PLF85_14780"
     tRNA            complement(595..667)
                     /locus_tag="PLF85_14780"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(632..634),aa:Asn,seq:gtt)
     gene            910..1554
                     /locus_tag="PLF85_14785"
     CDS             910..1554
                     /locus_tag="PLF85_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE20987.1"
                     /translation="MPRERLARWQKVQTSNLDQSGRSHLADSDKVFDRAAELQVPREG
                     DRLPLVLCDNIPKDVFFPADEQAVVAAIEASRVPEKSSLTHIYLRKPKASDFRNGRIP
                     LAEYVALDQYAVLIFYPWPRNLLMPLKKKPADNILNRYRRFEPSLVSHKGKWHLQWKE
                     DKVADFFLNELLPAELLVHAEFQAKHAARKSQKFIELPVQYANQRFFEENTVIW"
     gene            1618..1953
                     /locus_tag="PLF85_14790"
     CDS             1618..1953
                     /locus_tag="PLF85_14790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE20988.1"
                     /translation="MPREQKRHEGRLRVAVPGEYCFVGKDDWTPCRLNDLSADGMSLD
                     GKTSFYAGDKLEVRFPLEKRMILVTLEVTHLIGKKAGGRIIKIDDSDRRMIQEVLNRE
                     LMSGKTFLS"
     gene            complement(1955..2485)
                     /locus_tag="PLF85_14795"
     CDS             complement(1955..2485)
                     /locus_tag="PLF85_14795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE20989.1"
                     /translation="MHAHEDTSQQIVQYIDRLTAYAQSRLADKDLAADAVQEAITKAV
                     RSAASLKDEEKLLPWLYTILRNTIADLARKQAREIATDTLPEQAVEPDAGEQESLCSC
                     FRPLIATLPSDYGQVLQKVDLDEEPREQVAAQLGVSVGNLNVKLHRAREKLRQALENT
                     CNLCAKHGCLNCTCGG"
     gene            complement(2598..>3433)
                     /locus_tag="PLF85_14800"
     CDS             complement(2598..>3433)
                     /locus_tag="PLF85_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE20990.1"
                     /translation="GLNRLVERMHAVLHAVHEASDSIRDSSGRLNQTAHVLSTGTEET
                     SVQSRLIADATLEVSQKLESLSSAIQEMSISIGEISTHGAQAASIANEANKAVEASTT
                     TIDELGRSAQDISSVIEIISFIADQTKMLALNAAIEAAGAGEAGKGFSVVASEVKELA
                     RQTAESTEDIRRKVEAIQLRTEGAVQIVTQIGDLVSRLHGINGSIAATVEEQSMVVKE
                     IAAHVSSAAAAAHEVSNNIGGITTAAAEGAREASVSSGQAKSMLDLSGKLNQVVSGFT
                     V"
BASE COUNT          767 a          990 c          948 g          728 t
ORIGIN      
        1 caccgtacag gctcgaaccg agaatccaga cgggcacctg caacccctgc cccggcaccg
       61 cgcgcacctt gccctcgggt ttttcagaaa acaggttgag caactcgagc acgtcgtgcg
      121 gaaaattgtc tgcagcaagg tgtgcatttc ggcgcagtgc ctgcgccgtc agctgatcgg
      181 tacccggcgc acgccccagc cccaggtcaa tgcgtccagg gtaaagtgac tccaacgtgc
      241 caaactgctc ggcaatcacc agcggcgaat ggttgggcag catgatgccg cccgcgccca
      301 cgcgaatcgt ctttgtgccg cccgcgatat ggccgatcag caccgccgtt gccgcgctcg
      361 ccacccccgg catgccgtgg tgctctgcca gccagaagcg gcggtagccc cagcgctcgg
      421 cgtgctgcgc gagatcgagc gaatgcccaa acgactgcgc ggcattgctg ccctgcgtga
      481 tgggggcgag gtctagaatt gagagtggga gcatgtacgg caaatattca caggtttacc
      541 cagagcagca aaaaattctt aagagcgaag gggaaagtga atgcgagaag tatgctcccg
      601 aagcaggatt cgaacctgcg acacggtgat taacagtcac ctgctctacc gactgagcta
      661 ttcgggaata aataggtcgt cttgcgacat tgacctgccc cgaatggctc tgcctacaga
      721 gctattcggg aatatttcag gccgcgtagc ggcagcgcat ccgcttcgaa tagctctgct
      781 tacagtgcta ttcgggaatg gattagctgc aaggacgagc caaagatttt aacgcctcgt
      841 caatcattta gtagtggtaa caatcctatc tccgggtggt cagcttcacg cacccaaaaa
      901 tactgcccta tgccccgcga aagactggcc cgctggcaaa aagtgcagac atctaacctt
      961 gaccagagtg gccggtcgca cctggccgat tcggacaagg tatttgaccg ggcagcggag
     1021 ttacaggtgc cacgtgaggg cgaccggctg ccgctggtgc tctgcgacaa tatacctaaa
     1081 gatgtattct ttcctgccga tgagcaggcg gtcgtggccg ccatcgaggc cagccgggta
     1141 cccgaaaaat cgagcctgac gcacatctac ctgcgaaaac cgaaagcgag tgactttcgc
     1201 aacgggcgca ttccgctggc cgaatatgtt gcgctcgacc agtacgcggt gctgattttt
     1261 tatccgtggc ccaggaacct gctgatgccg ctgaagaaaa agccggccga taatatactc
     1321 aaccgctacc gtcggtttga acccagcctc gtcagccata agggcaagtg gcacctgcaa
     1381 tggaaagaag acaaggtggc ggattttttc ctgaacgagt tactaccggc cgagctgctg
     1441 gtacacgccg agttccaggc gaaacatgca gcgcgcaaga gccaaaagtt catcgagttg
     1501 ccggtgcagt atgccaacca gagattcttt gaggagaata cggtaatatg gtgaccaacg
     1561 gggagtttca gtcagcaggc acgaaatcct ataatcaagt agtttatcag acaatctatg
     1621 ccccgtgaac aaaaacgtca tgaaggcagg ctgagagtcg ccgtaccggg agaatactgc
     1681 ttcgttggta aggacgactg gacaccctgc cggctgaacg acctgagcgc cgatggcatg
     1741 tcgctcgacg gcaaaacttc tttttatgca ggagacaagc tagaggtacg tttcccgctc
     1801 gaaaagcgca tgattctcgt gacgctcgag gttacacacc tgatcggcaa gaaggccggc
     1861 ggaaggatta tcaagatcga cgattcagac cgccgcatga tccaggaagt cctgaaccgt
     1921 gagctgatgt caggcaagac ctttctctct taagttatcc gccgcacgta cagttgaggc
     1981 agccatgctt tgcacagagg ttgcaggtgt tttcgagcgc ctgccgcagc ttctcacgcg
     2041 cacggtgcaa cttcacattc agattaccca cagagacacc taactgtgcg gccacctgct
     2101 cgcgcggttc ttcatcgagg tcaacctttt gcagtacctg cccataatca gacggcaagg
     2161 tagcgatgag cggcctgaaa caactgcata gactctcctg ttcgccagcg tctggctcaa
     2221 cagcctgctc aggcagcgta tcagtcgcaa tctcgcgggc ctgtttacgc gccaggtcgg
     2281 caatcgtatt gcggagtatt gtgtagagcc agggcaacag tttctcttca tctttcaggg
     2341 aggcagcaga tctgacagct ttggttatag cctcttgcac cgcgtcggcg gcgaggtctt
     2401 tgtcagccag ccggctctgc gcgtaagcag tcaggcgatc gatatactgc acgatttgct
     2461 gcgaggtatc ttcgtgcgca tgcatagatg acgcgactac cggggatagt cagccgtgca
     2521 atcgtttcgt ggcttacctg acgatacatg ccacaccggc tgttgcggaa ttaccaccac
     2581 ggtacagcac gtgactgtca gacagtgaag ccgctgacta cctggttcag cttacccgag
     2641 aggtcgagca tcgattttgc ctgccccgac gagacacttg cctcgcgcgc gccctcagct
     2701 gcggcagtgg ttataccgcc gatattgttg ctaacctcat gcgccgcagc tgcggcagag
     2761 ctcacgtggg ccgcgatttc tttcaccacc atcgactgtt cttcgacggt cgctgcaatg
     2821 ctgccattga taccatgtag ccgcgatacg agatcgccaa tttgggtgac aatctgcacc
     2881 gcgccttcgg tccttagctg tatcgcctcg accttgcgcc tgatatcttc tgtcgattcg
     2941 gctgtctggc gcgcgagctc ttttacttca gacgccacaa ccgaaaaacc tttgccagct
     3001 tcgccggccc cggctgcttc aatcgcggcg ttcagggcca gcatcttggt ctggtctgca
     3061 ataaaagata tgatctcaat caccgaactg atatcctgcg cagaacgacc taactcatcg
     3121 atcgtggttg tcgaggcctc aacagccttg ttggcctcat tcgcgatact cgccgcctgt
     3181 gcgccgtgcg tagaaatctc accgatgctg atcgacattt cctggattgc actggataac
     3241 gactcgagct tctgcgagac ttcaagcgtc gcatcggcaa tgagccgcga ctggacagac
     3301 gtctcttcgg taccggtcga gagaacgtgt gcggtctggt tcagacggcc tgaagaatcg
     3361 cgtatcgaat cgctcgcttc gtgaactgca tgcagtacgg cgtgcatgcg ctccacgaga
     3421 cggttaaggc cac
//
LOCUS       DAOQJF010000354         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17678_length_3430_cov_5.210370, whole genome shotgun sequence.
ACCESSION   DAOQJF010000354 DAOQJF010000000
VERSION     DAOQJF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17678_length_3430_cov_5.210370"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1112
                     /locus_tag="PLF85_14805"
     CDS             108..1112
                     /locus_tag="PLF85_14805"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNE20991.1"
                     /translation="MALFDECFSSEADPSLAPRDMEKFWNGQIARLKKIPLDIQTKKK
                     VSRKLFTEQNLMMDFQSIDKYHMQAQFIAPRKLIGKPPLVVIFPDYGSESIAYKGLMN
                     AGIAQMVVRMRGHEAPRPSQIQEKNAVKNKEERARSYGYFGEHLLDPEDYYATKIYLD
                     AYRSLEVARLRKEIDTSRIGIIGQGIGAAQALFVAAFMQRGDAIYLENPAFLNLEQTQ
                     NLSEADYAREINEAAKGSKKVKQQIKENLRYFDPVYFANRIGASTAFSVNLTNKHSVP
                     HGSFAMFHLLRNEKDMFLFTEGDPVALAEEKRKTASNAVRYFKEKLLGQVSDAPMSDD
                     "
     gene            complement(1116..1469)
                     /locus_tag="PLF85_14810"
     CDS             complement(1116..1469)
                     /locus_tag="PLF85_14810"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNE20992.1"
                     /translation="MQPLKIGIDRLKIKTSVGIYAGEKKKKQLLWISAVLSYRHVGYH
                     IDDMSSSMDYDILCNEIRRIAALRHYELIENLALHAGQALKKLARCDSVEIRIDKPLA
                     ARKNSAQSIWVVVET"
     gene            complement(1444..2457)
                     /gene="galE"
                     /locus_tag="PLF85_14815"
     CDS             complement(1444..2457)
                     /gene="galE"
                     /locus_tag="PLF85_14815"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNE20993.1"
                     /translation="MAKDNRNVLVTGGAGYIGSHAVRALRAAGYSPVILDNFSTGHRE
                     FVSDFPLVEGSVGDVSVIRRAVAEYKISAVMHFASFINVGDSVHAPAEYYRNNVAEGV
                     SLIAALKGCGVRRFILSSTCAVYGAVEGVRALTESDKIAPLNPYAHAKRMLEIVLTDM
                     AHAGEMDSVIFRYFNAAGAAASNETGEWHEPETHLIPNLLRSALTGGKNPLRVFGNDY
                     AIPGRDGTAVRDYIHVADLADAHVRGLGYLESHPGSTIINLGTGNGASVLEVVRTAEH
                     VLGQKISFEYHPRREGDAPYLVGDNRRAQQLLGWTPENNLERILRSAWEWEKKLAALK
                     NRH"
     gene            complement(2457..>3430)
                     /locus_tag="PLF85_14820"
     CDS             complement(2457..>3430)
                     /locus_tag="PLF85_14820"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNE20994.1"
                     /translation="ELTNYAIEMEARLRAADPQIENVPYTGASRSSSTGYFLNSHGVR
                     YRITHEDFGAYTAAVAARGDQKKMGFASNARTEFTELCALPLTSLAAERALVQLGASP
                     VTSGTYNVLFSNRVSGQLFSLYQSPFFAETVQRGQSRLAGKLGQPVASERLSLRSDAL
                     DTTLRGSRALDSEGVPTSNLAVIESGKLVNYLYNLEAAAQDKRASTGNGVRSVGSRAG
                     TGFKNLVVAPGDKTRAELLQSGRILLIDKLEGAAGCSAISGEFSIGAQGFLYENGQMV
                     QPVDRITLSSNFFEMLRNIEAVSSEYNDQYSSVRVPDLLIGGIAVAG"
BASE COUNT          807 a          936 c          920 g          767 t
ORIGIN      
        1 cgtggggcat ggttaactgc agaattgcgc ccgcccgcgg caatcattcc gaataccggg
       61 agcggcaaaa gtatgtacgc ggccattcac ctctacaatg tgaacgaatg gcgctctttg
      121 acgaatgttt cagctctgag gcagatccat ccctggcacc gcgggatatg gagaaattct
      181 ggaacgggca gattgcccgg ctgaagaaaa tacccctcga tatccagaca aaaaagaagg
      241 ttagccggaa gcttttcacc gagcaaaacc tgatgatgga tttccagagt atagacaagt
      301 accacatgca ggcgcagttc atcgcccccc gcaagctgat cggcaaaccg ccactggtag
      361 ttatttttcc cgattacggc agcgagagta ttgcctacaa gggcctgatg aatgcgggta
      421 ttgcgcagat ggttgtacgc atgagggggc acgaagcgcc acggccttcg cagatacagg
      481 aaaaaaacgc ggttaaaaat aaagaagagc gtgcgcgcag ttacggctat ttcggtgagc
      541 acctgctcga ccccgaagac tattatgcta ccaagatcta tctcgacgcc taccgctcgc
      601 tcgaggttgc ccgcctgaga aaagagattg atacttcacg catcggcatc atagggcagg
      661 ggatcggtgc cgcccaggca ttgttcgttg ccgctttcat gcagcgaggc gacgctatct
      721 accttgagaa ccctgccttt cttaacctcg agcagacaca gaacctgtca gaggctgact
      781 acgcgcgcga aattaatgaa gctgcaaagg gtagcaaaaa ggtaaagcag cagatcaaag
      841 aaaacctgcg ctactttgac ccggtatact ttgccaaccg cataggggca agcacggcat
      901 tttcggtaaa cctgacgaat aaacattcgg tgccgcatgg ttctttcgcg atgtttcacc
      961 tgctgagaaa tgaaaaagat atgttcctct ttaccgaggg ggaccctgta gcgctggctg
     1021 aagagaagcg caagacggct tcgaacgcgg tacgctattt caaagaaaag ctgcttggcc
     1081 aggtgagcga cgctccgatg agcgacgatt aaagctcagg tttcgacaac aacccatata
     1141 ctctgcgcgc tgttcttacg cgctgccagt ggcttgtcga tgcggatttc taccgagtcg
     1201 caccgcgcca gtttcttaag cgcctggcct gcgtgcagcg caagattctc tatcagctcg
     1261 taatgcctga gggcggcaat gcgccgtatt tcgttgcaga ggatgtcgta atccatcgaa
     1321 gaagacatgt cgtcgatgtg gtagcctaca tggcggtaag aaagtactgc cgatatccag
     1381 agtaactgtt tcttcttttt ttcgccggca taaataccga ctgaagtttt tatcttgagg
     1441 cgatcaatgc cgatttttaa gggctgcaag ttttttctcc cattcccacg cactgcgcag
     1501 gattctctcc aggttatttt caggtgtcca gccgagtaat tgttgcgccc ggcggttatc
     1561 gccgaccagg tacggggcat cgccctcgcg tctcggatgg tattcaaacg aaatcttttg
     1621 ccccaggaca tgctctgcag tgcgtaccac ttcaagcacg ctggcgccgt taccagtacc
     1681 caggttgatg atggtgcttc cgggatggct ttcaaggtaa ccgagtcccc tgacgtgcgc
     1741 atcggcaaga tccgctacat ggatgtagtc gcgtactgct gtaccgtcac gcccgggaat
     1801 tgcgtagtcg ttgccaaata cccggagcgg attcttacca ccagtcagtg cagaccgtaa
     1861 aaggttggga atcaggtggg tctcaggctc atgccattcg cctgtttcgt tggatgccgc
     1921 agcgcccgcg gcattaaaat agcggaagat caccgaatcc atttcacctg cgtgagccat
     1981 atcagtaagc acgatctcca gcatacgctt tgcatgcgcg tatgggttta gcggtgctat
     2041 cttgtctgac tctgtgagtg cacggacacc ttcaacggcc ccgtaaaccg cacaggtcga
     2101 agagagaata aacctgcgca caccgcagcc ttttaatgcc gcgattaacg acacaccctc
     2161 ggccacattg ttacggtagt attctgcagg tgcgtgtact gaatcgccca cgttaatgaa
     2221 cgaggcaaag tgcatgaccg cgctgatctt atattctgcc actgcgcggc ggatgacaga
     2281 cacatcgccg acactgcctt cgacaagggg aaagtccgac acgaattcac ggtgaccagt
     2341 gctgaaatta tccaggataa caggcgaata gcccgctgcc cgcagcgcgc ggaccgcatg
     2401 cgatccgatg taaccggcgc cgcctgttac caggacattg cggttgtctt ttgccattag
     2461 ccagccaccg caataccacc tatcaacaga tcgggcaccc gcaccgagct gtactggtcg
     2521 ttgtattcag aagagactgc ctcaatgttg cgcagcatct caaaaaagtt ggagctgagg
     2581 gtaatacggt caaccggctg taccatttgg ccattctcgt aaagaaagcc ctgtgcacca
     2641 atggaaaact cacccgaaat agcggagcag ccagctgcgc cttcgagctt atcaatcagt
     2701 agtatgcggc ccgattgcag cagctctgcg cgggttttat ctcccggcgc aacgaccaga
     2761 ttcttgaagc cggtccctgc cctcgagccg acagaacgga cgccattacc ggttgaagct
     2821 cgcttgtcct gggcggctgc ttcgagattg tacaggtagt tgaccagttt gcccgattca
     2881 atcaccgcca ggttacttgt tggcacaccc tcggaatcca gagcacgcga tccgcgcagc
     2941 gtggtatcaa gagcatcaga gcgcaggctg agtctttccg atgcaaccgg ttgccccagc
     3001 ttacctgcca gccttgattg ccctctctgc acggtttcgg caaagaaggg cgactggtag
     3061 agtgagaaga gttggccact cacacggtta ctgaacagta cgttgtaggt tcctgacgtt
     3121 acggggctcg ctcccagttg aacgagcgcg cgttctgcag ccaatgatgt cagggggagt
     3181 gcgcaaagtt ctgtgaattc ggtacgcgcg ttgctggcga atcccatctt cttttgatcg
     3241 cctcttgccg caacagcagc ggtataggcg ccaaaatctt catgggtgat gcggtaacgg
     3301 acgccatgcg aattcagaaa ataccccgtc gagctcgagc ggctagcgcc ggtatagggt
     3361 acattctcga tctgcgggtc tgccgcgcgc agcctggctt ccatttcaat ggcataattc
     3421 gtcagctcag
//
LOCUS       DAOQJF010000355         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17713_length_3426_cov_4.513497, whole genome shotgun sequence.
ACCESSION   DAOQJF010000355 DAOQJF010000000
VERSION     DAOQJF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17713_length_3426_cov_4.513497"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLF85_14825"
     CDS             <1..497
                     /locus_tag="PLF85_14825"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE20995.1"
                     /translation="VGAQPADRIREILDQFIVPEDYLKVLNLSQNSPAEARSLLQKLK
                     GKRLEEAAWAVARGFLVQQAADAAAASDCLQLIPEFGSAFSEARTAALAMLSDGQTAE
                     LATLIAGKEENTTPLLDRWIEQVEESKDKQKDKEILIRAFHILGNEHPLTLDYRRKLS
                     RVLF"
     gene            593..1672
                     /locus_tag="PLF85_14830"
     CDS             593..1672
                     /locus_tag="PLF85_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE20996.1"
                     /translation="MRWSIPIGEFRGIKVYIHWTFFLLLTWIGLSSYRAAQNTDQALL
                     TIGYILTLFGCVVLHEFGHALMARRFGIVTRDITLFPIGGMARMEKMPEKPMQELLVA
                     IAGPMVNVVIAAGLFAYMHFSPTVYMLEFPKGPMTPELFLPTLFLVNVLMAVFNLIPA
                     FPMDGGRILRALLALKFDRARATQIAANLGQVIAIGFVFLGLFYNIWLVFIGIFIYLG
                     AGAEASQEYVKHDLGVHNVGSLVMRKFTVLRAEETLSHALRALLDSQEREFVVTENGA
                     VAGILTRDEMLAGLSRFGQTVPIGQIMRRDIRILPPQMPVTEALQLMNELNLPMLPVG
                     YEGQLMGVIDVENISEFLLIQKAMR"
     gene            1682..2134
                     /locus_tag="PLF85_14835"
     CDS             1682..2134
                     /locus_tag="PLF85_14835"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNE20997.1"
                     /translation="MAYGKKFRVPGLKRSDSFADAQQPVLSHYLKRVLKQAKKFSKDG
                     TAEHLHNLRIALRRFRYVLELYAAEFKPKKFKAIYELAIRLQNTLGDRRDIDVMHERL
                     LSLYRSGEGELPALISADLEQSKALLNTQIEQTLAEFVRNPQLRKLVD"
     gene            2140..2844
                     /locus_tag="PLF85_14840"
     CDS             2140..2844
                     /locus_tag="PLF85_14840"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNE20998.1"
                     /translation="MDRLFWRFGQETGWGPQQLGAVGLPAGGTDVWFFENTENFQYEG
                     VVSDTELARAQDMLPTAKGQEFLSFRACLRIVLALYYIKTIAPKNIRIDISEHGKPWL
                     PDASQLHFNLSHSHGALAIAVSRLEVGVDIEKIRPIPDWQDLAKNFLAPTDTATIANH
                     AEAERSAAFLRCFTAREAYLKALGTGFSAPLPAMDFHSSYREQFTTDDRECLLAPLPP
                     LPGFVGHVCIVNRGIA"
     gene            2986..>3426
                     /locus_tag="PLF85_14845"
     CDS             2986..>3426
                     /locus_tag="PLF85_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE20999.1"
                     /translation="MTDIKNAIIKKPADLAKHHLTSYDAYRRDFSWDKIAAEIGKNGD
                     FNMARITIDRHVEAGRGNTTAIRWLGSEDSKIDISYAEVQKQTNRFANALASLGIARG
                     DKVFALSGRLPGLYVSALGSLRHGAVFSSLFSAFGPDPIATRMTI"
BASE COUNT          769 a          997 c          921 g          739 t
ORIGIN      
        1 tcgtcggggc acaaccggct gaccgtatcc gcgaaattct cgatcagttc attgtgccgg
       61 aagattacct aaaggttctg aatctcagcc agaactcacc cgcagaagcc aggtcactgc
      121 tgcaaaagct gaagggcaaa cgactagaag aagcagcctg ggctgttgca cgcgggttcc
      181 tcgtacagca ggctgcagat gcagcggctg cttcagactg cctgcaactg attcctgaat
      241 ttggctcggc gttcagcgaa gcgcgcaccg ctgcgctggc gatgctcagc gacgggcaga
      301 ccgcagagct cgccacccta atcgccggca aagaagagaa taccacaccc ctgctcgacc
      361 ggtggatcga acaggttgaa gaaagcaaag acaaacaaaa agacaaagaa attttgatac
      421 gggcatttca tatcctcggt aatgagcatc cgctgacact tgactaccgc cgtaaactgt
      481 cccgagttct attctgacct cgcgaactga caggctggcg gtagccagtc tctcagttcg
      541 tgaagacgcg gtttctgcat gacgcagagt tgcacaccga cagtacgtac ccatgcgctg
      601 gtcgatacct ataggtgaat ttcgcggtat taaggtctac atccactgga ccttcttttt
      661 actcttaacg tggatcggcc tcagcagtta ccgcgcagca caaaacaccg accaggcgct
      721 gctgaccatt ggttatattc tcaccctttt cggttgcgtc gtgctgcacg agtttggcca
      781 cgcgctgatg gcccgccgct ttggcatcgt cacccgcgac atcacgctct tcccgattgg
      841 tggcatggcg cgcatggaaa aaatgccaga gaagccgatg caagagctgc tcgtcgcgat
      901 cgccggcccc atggtcaatg ttgtgattgc cgctgggctc ttcgcttata tgcatttttc
      961 accgacagtc tacatgcttg aatttccgaa ggggccgatg acgcccgagc tttttctgcc
     1021 aacgctcttt ctcgtgaacg tgttgatggc agtctttaac ctgattcccg cctttccgat
     1081 ggatggtggc cgcattctgc gcgccctgct cgccctgaaa tttgaccgcg cccgcgccac
     1141 ccagattgcc gcgaacctgg gccaggtgat cgcgatcggc ttcgtcttcc tgggcctgtt
     1201 ctacaatatc tggctcgtct tcatcggtat ctttatttat ctgggtgctg gcgccgaagc
     1261 cagccaggaa tacgtgaagc acgatctcgg cgtgcacaat gtcggcagcc tcgtgatgcg
     1321 caaattcacc gttctacgtg ctgaagagac tctgagccac gcgctgcggg cgctactcga
     1381 cagccaggag cgcgagtttg ttgtgacgga aaatggcgct gtcgccggca ttctgacgcg
     1441 cgatgaaatg ctcgccggac tctcccgttt tggccagact gtaccgatcg gacagatcat
     1501 gcgccgtgat atccgcattt tgccgcccca gatgcccgtg acagaagccc tgcagctgat
     1561 gaatgagctg aacctgccga tgctgccggt tggttatgaa ggccagctga tgggagtcat
     1621 cgatgtcgag aacatcagcg agttcctgct gatccaaaag gcaatgcgct gagaggcagc
     1681 catggcatac gggaaaaagt ttcgtgtccc ggggctcaaa cgctcagact ctttcgccga
     1741 tgcgcagcaa cctgtgctat cccattacct caagcgcgta ctgaagcagg cgaaaaaatt
     1801 ttccaaagac ggaacagccg agcatctcca taacctgagg attgccctca ggcgctttcg
     1861 ttacgtgctc gaactctacg cggcagagtt caagccgaaa aaatttaagg ccatctatga
     1921 actggcaatc agattgcaga acacgctcgg tgatcggcgc gatatcgacg tcatgcacga
     1981 acgcctgctc tcgctgtacc gcagcggcga aggggagcta cctgcactga tcagcgctga
     2041 tctcgaacag agcaaagcac tgctgaatac gcagatcgaa cagacactcg ccgagttcgt
     2101 cagaaatccg cagctacgaa aactggtcga ctaacctgca tggatagact cttctggcgg
     2161 tttggccaag agaccggctg gggtccgcag cagctgggtg ccgtcggtct gcctgccggc
     2221 ggtacggatg tctggttttt cgagaataca gaaaatttcc agtacgaggg tgttgtttca
     2281 gatacagagc tggcgcgcgc gcaggacatg ctgccaaccg caaaaggaca ggagttccta
     2341 agctttcggg catgcctgcg catcgtgctg gctttgtact acataaagac catcgcccca
     2401 aaaaacattc gcattgatat ttctgagcac ggtaagccct ggctacccga tgcgtcgcaa
     2461 ctgcacttca acctctcgca ttcgcatggc gcgctggcga tcgccgtgag ccggctcgag
     2521 gtcggtgtcg atatcgaaaa aattcggccc atacccgact ggcaggacct ggctaagaac
     2581 ttcttagctc ccactgatac tgcaaccatc gccaaccatg cagaggcaga acggagtgcg
     2641 gcttttctgc gctgttttac cgcgcgtgag gcgtacctca aggcactcgg cacaggcttt
     2701 tcggccccgc tgccagctat ggatttccat agcagctata gagagcaatt tacgacagac
     2761 gacagagaat gcctcctggc gccgttacca ccattgccgg gcttcgtcgg gcatgtctgc
     2821 atagttaaca gaggcattgc gtaagtgcta gctgccgcga cctcttgcgc agagcgcaac
     2881 ggccctctga aaaaagaatt atacatccag tattccactt gccagatcac acgctcaccg
     2941 cagggtgtac ccacaggggt tactgaatcc ttgcccggag atcacatgac cgacattaag
     3001 aatgccatca tcaagaagcc agcagacctg gcaaaacacc atttgaccag ctacgatgcg
     3061 taccgccgcg acttcagctg ggacaaaatc gccgccgaga tcggcaagaa cggcgacttc
     3121 aacatggcgc gcatcacgat cgaccgccat gtcgaggcag ggcgcggcaa cacgactgcg
     3181 atccgctggt tggggtcaga agactcaaag attgacatca gttacgccga ggtgcaaaaa
     3241 cagaccaacc gttttgccaa tgcactcgct tcgctcggca ttgcgcgcgg cgacaaggtc
     3301 tttgcactct cgggtcgcct gccgggcctc tatgtctctg cgctcggctc gctgcgccac
     3361 ggcgcggttt tttcttcttt gttttctgcg tttggccccg acccgattgc gacgcgcatg
     3421 acgatt
//
LOCUS       DAOQJF010000356         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_17902_length_3405_cov_6.115522, whole genome shotgun sequence.
ACCESSION   DAOQJF010000356 DAOQJF010000000
VERSION     DAOQJF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17902_length_3405_cov_6.115522"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLF85_14850"
     CDS             <1..326
                     /locus_tag="PLF85_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNE21000.1"
                     /translation="KGHRYVFEHAKVMIHQPLISGQIVAPAIDIKIHAEEIKKTREEL
                     NRIIADTTGQPLTRVEKDTDRDYYLDGKGAVEYGLADKVLTDFSELGLFDSKPAKTAK
                     PKKTK"
     gene            complement(348..1544)
                     /locus_tag="PLF85_14855"
     CDS             complement(348..1544)
                     /locus_tag="PLF85_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE21001.1"
                     /translation="MTRYQVKLLRPGVSFTKPVYIDPTNMLAAANVIVTQSDLDRLNK
                     WKIEEVLSAGEPVYASKKDEAEFMDGQQKLDIENLKKELKNLIRAKPAFNSLTSSTEA
                     VLKNFYERLNSNNNPQSNEVRSKAEEITNLVTANPLIVTFVAEYIIDADIYKHVVGCS
                     MYATRLGLALDFSRPKIVEMIFAILMMDVGMLKVPQAILEKAEKLNETELQIIHAHPV
                     HGYQMLTQIAKIKNTIASVALQHQEHFDGSGYPRKIKGAEMAEYTRVASIIDSFAAII
                     ARKPYRKNRLPYDAMKELLTLGIYRYDPVYLKTFLDSHAIYPLGSIVELSDGSTGMVV
                     SAVKGKPMRPIVWILRDARNQRPARPEFAHLLYASDKFISKAKYAEDAGLNLDMEFEQ
                     LMKQLG"
     gene            complement(1704..1994)
                     /locus_tag="PLF85_14860"
     CDS             complement(1704..1994)
                     /locus_tag="PLF85_14860"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNE21002.1"
                     /translation="MNPAGQGKLHEILRACALEFRGEDAPVVRWYAEGDAARRLLELA
                     RQRGVRIEEDQAEELLLALRGVKLNSRIPREIYLAVAKIYAYILSQEKPRPD"
     gene            complement(1997..2548)
                     /locus_tag="PLF85_14865"
     CDS             complement(1997..2548)
                     /locus_tag="PLF85_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21003.1"
                     /translation="MEVKRSDMLKWAQNAQMSTQASDSQAADRPAEVRTDRPGQVNVD
                     SMRSKILSLQQEIRELQTGISMRQIQLGFLNQVNEGGSWQKDLRRFMNEQFPTVQMQL
                     GEGQNIDEYRAEAAQVVSSLRNNLIKKEIQIQNILSAGLFEPAAESDEEPVLDNSKII
                     KDFSETQGIFSKLRPDAVRTLVN"
     gene            2762..2833
                     /locus_tag="PLF85_14870"
     tRNA            2762..2833
                     /locus_tag="PLF85_14870"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2794..2796,aa:Pro,seq:tgg)
     gene            2909..2982
                     /locus_tag="PLF85_14875"
     tRNA            2909..2982
                     /locus_tag="PLF85_14875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2943..2945,aa:Arg,seq:tct)
     assembly_gap    3212..3311
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          721 a          889 c          913 g          782 t
ORIGIN      
        1 aaaaaggcca ccgctacgtg ttcgaacatg ccaaggtcat gatccaccag ccgctgatta
       61 gcgggcaaat cgtcgcgccc gcgatcgaca tcaaaattca cgcagaagaa atcaagaaaa
      121 cacgcgaaga actcaaccgc attatcgccg acacgaccgg gcaaccactg acgcgcgttg
      181 aaaaagacac cgaccgcgat tattacctcg atggcaaagg tgccgttgag tatggcctgg
      241 cggacaaggt gctcacagac ttttctgagt tgggcctgtt tgattcaaag ccggcaaaaa
      301 ctgcgaaacc caagaagaca aaatagcggg ctaatgcccg cactctttta gcccagctgt
      361 ttcatcagct gttcaaattc catatcgaga ttgaggccgg cgtcttcggc atacttggcc
      421 tttgaaatga acttgtctga cgcgtagagc aggtgcgcaa actcaggccg tgcggggcgc
      481 tggttcctgg catcgcgcaa gatccagacg atgggccgca tcggttttcc cttcacggca
      541 gagacgacca tgccggtgct gccatcggaa agctcgacta tcgagccaag gggatatatc
      601 gcatgcgaat cgagaaacgt cttcagatag accggatcgt accggtaaat accgagcgtg
      661 agcaattctt tcatcgcatc atacggcaga cggttcttgc ggtacggttt gcgcgcgatg
      721 atcgcggcga atgagtcaat aatcgaagcc acgcgggtgt attcagccat ctcggcgccc
      781 ttgatcttgc gcgggtaacc cgaaccgtca aaatgctcct ggtgctgcag ggccacagag
      841 gcaatggtgt tcttgatttt ggcgatctgg gtcagcatct ggtagccgtg taccgggtgc
      901 gcgtgaatga tctgtagctc tgtctcgttc agcttctcgg ctttttccaa aatcgcctgc
      961 ggcaccttga gcatgccgac atccatcatc aggattgcga agatcatctc gacgatcttc
     1021 ggccgcgaaa aatcgagcgc aagccccagg cgcgttgcgt acatcgaaca gccaacgacg
     1081 tgcttataga tatcagcgtc gatgatgtac tctgccacaa aggtgacgat gagagggttg
     1141 gccgttacga ggttcgtgat ctcttcggct ttgctgcgca cctcgttgga ttgcggattg
     1201 ttattgctat tgagccgctc atagaagttc ttcagcaccg cttcggtcga tgaagtcagg
     1261 ctgttgaagg ccggttttgc ccgaatcagg ttcttgagct ctttcttgag attttcaata
     1321 tccagctttt gctggccgtc catgaactcc gcttcgtctt tttttgaagc atagacaggt
     1381 tcacctgccg acaggacttc ttcaattttc cacttgttca ggcggtcgag gtcactctgc
     1441 gtaacaatga cgttggccgc ggccaacata ttggtcgggt caatatatac gggtttggtg
     1501 aaagaaacac ccggacgcaa taatttgacc tgataccggg tcatagtcta ttctcggtat
     1561 aaacttgcct gatttgaagc ggatttatcg tttttcgtga aatgtgcgcc gtcagcacat
     1621 agtgtaccgg gcggggcaca gttctcggga tatacatcgc attagagctt accgccagca
     1681 gcgatttcgt ccgtaaaatc atttcaatca ggccttggtt tttcctgtga aagaatataa
     1741 gcatagatct tcgcaactgc cagatagatt tcgcggggaa tgcggctgtt gagtttgact
     1801 ccccgcaggg caagcagcag ttcttcagcc tggtcttctt cgatgcgtac gccgcgttgg
     1861 cgcgccagct cgagcaggcg cctggcggca tcgccttcag cgtaccacct cacgacaggg
     1921 gcgtcttcgc cacgaaactc gagcgcacac gcgcgcagaa tttcgtggag tttgccctgc
     1981 cccgccgggt tcaagcctaa ttgaccagcg tcctgacggc gtctggtctc agcttgctga
     2041 agatgccctg cgtctcggag aagtctttga tgattttcga gttatcgagg acgggttctt
     2101 cgtcactctc tgctgcaggc tcaaagaggc cggctgacag tatgttctga atctggattt
     2161 cttttttgat caggttattg cgcaggctgc tcacgacctg tgctgcttct gcacggtatt
     2221 cgtcgatgtt ctgcccttcg cccagctgca tctgcactgt cggaaactgt tcgttcatga
     2281 agcggcgcag gtctttctgc caagagccac cctcattcac ctggttcagg aaacccagct
     2341 ggatctgccg catcgagatt cctgtctgta attcgcggat ctcttgctgc aggctgagaa
     2401 ttttgctgcg catcgaatca acattaacct gccccgggcg atcagtgcgg acttcagccg
     2461 gccggtctgc agcctgcgag tcgctggcct gggtactcat ctgcgcgttc tgcgcccatt
     2521 tgagcatgtc gctgcgcttc acttccatac gcacttctgt tgcgctttgc acagattgtc
     2581 aatcttttcg acaatcgcca ggctacccgg ctgagccacg caagcgagac ctacttgcct
     2641 tgctggcggt ccctcagcgc ggttggtagc ccagaggagg ccgctcagca cccccgtatt
     2701 tctttccgta ccggctattc tcttgacgcg gtgtctcagg cccctgttta gccagcgtca
     2761 acgggatgta gcgcagcggt agcgcacttg ctttgggagc aaggggtcgc tggttcaaat
     2821 ccagtcatcc cgacatttta gcgcatgggg cagaggcctg gtgcggaacg cgaccacgcg
     2881 gacgcaagca cctagaggtg cgcaatatgc gcacgtagct cagttggata gagcagcagc
     2941 cttctaagct gcgggtcagg ggttcgactc ccttcgtgcg cgcaattgag attttttgag
     3001 tttgagccgc gctcaaaccc aaaagaagaa aaaggtgatc gtagcaccga ggacggaggc
     3061 cgaagacggc cacagctggt agagcacagc tgactacgat agaaaatatg gtgatcgtag
     3121 caccgaggac ggaggccgaa gacggccaca gctggcagag cacagctgac tacgatagaa
     3181 aatatggtga tcgtagcacc gaggacggag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn ncggccacag ctggcagagc acagctgact acgatagaaa atatggtgat
     3361 cgtagctcag ctggtagagc acaggtttgt ggcacctgag gtcgc
//
LOCUS       DAOQJF010000357         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18010_length_3391_cov_5.336031, whole genome shotgun sequence.
ACCESSION   DAOQJF010000357 DAOQJF010000000
VERSION     DAOQJF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18010_length_3391_cov_5.336031"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLF85_14880"
     CDS             <1..425
                     /locus_tag="PLF85_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21004.1"
                     /translation="IRVTRDGRWFTGAKEIINYKVISHFRQHLFRDEKGIYIYQTFRQ
                     FAEKGYITVEGPLLSVFRADEVNLTLDSLDTLPVAEAQVVQNSDDEALYVYYPRLGCY
                     ASVPASVSPDFARLLSEEGERILFCGRAIEAQPITAWS"
     gene            complement(420..1316)
                     /locus_tag="PLF85_14885"
     CDS             complement(420..1316)
                     /locus_tag="PLF85_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNE21005.1"
                     /translation="MGTLLSATLICDRPEQCDVGFTAISDVLSDIDSRMSVYHTSELT
                     RLNAAAGTASLLQPLSTSTLEVLRTALVIAAQSQGRFDPTILPLIKAYGLMRENFGGE
                     SDTAQVKLVEAGSLVDYRQVLISGTRAGLKKKGMALDFGGIAKGYALDQVAVVLRKLG
                     IGAFVLNFGGHLLVHGTEHTTTVYDPQEANRVLVSCTISEGSISVSAQDKRYVHERGK
                     RRGHLINPLTKESESDTVLSLVYHPSAMHADGWSTALFFTRTTEFNALAKKQQLTALH
                     LSSRGELSHSEQMQGLKLCTPL"
     gene            complement(1334..1879)
                     /locus_tag="PLF85_14890"
     CDS             complement(1334..1879)
                     /locus_tag="PLF85_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21006.1"
                     /translation="MFTSRFRIFFYGTTWLSTTTGLALVYFMLQPADFFPTLRQALLK
                     THVAAVVTWLFLCGMLFSIHVIPQLVAQIRQGLRTGIALIGLVAAMTLSGFALQVIPW
                     PPVLETARWIHFYLSVLFVLLLIIHLLLVRPELKSWVLAIAVISLLLLLPLSTLKKTE
                     VFPDEIQLTPRSDVLPEKKTP"
     gene            complement(1879..2412)
                     /locus_tag="PLF85_14895"
     CDS             complement(1879..2412)
                     /locus_tag="PLF85_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21007.1"
                     /translation="MLARKSIFVLTLLITQAEARVYRTFPEAIQSVFPQADNLKRRSV
                     YLTPEQLKRAVQYANEPIDTAFLVVYEITKAGKLIGYAYLDTHRVRTLNETVLICIDE
                     KERILHIEQLSFAEPQEYMASDAFLSLFHGQTLSPALSLKGNIPTITGSTLTCSVLTS
                     SARRVLALHKIVLEQKR"
     gene            complement(2551..>3391)
                     /locus_tag="PLF85_14900"
     CDS             complement(2551..>3391)
                     /locus_tag="PLF85_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21008.1"
                     /translation="LHDPRLNFRAGLLLIPMGIYNEMHEAPTFLAATRPLTETVIMPS
                     TWRENGAGIFGQLGDFAYKLYGVTSLNAMTDPTNGTPKQPNFTSQGLRGGRQRGNEAI
                     ANEFSGVLRVDYTGFTGSIFGVSGYYGKSGSADLHMHETTILDAHADVRFRGVYFRSV
                     VTFANLTGADRLRTASGSTTIGPADQMYGAYIELGYDVFRLLNITEQELYPHIRLEKV
                     DTQYKVAAGNRFDARYEQNVLTIGLAYKPIRQIIAKIDYAITSNKAQTGFNQLNFGLG
                     YLF"
BASE COUNT          790 a          880 c          964 g          757 t
ORIGIN      
        1 acatacgcgt aacacgcgac ggccgctggt ttaccggcgc caaagaaatc atcaactaca
       61 aggttatcag ccatttcagg caacacctgt ttcgcgacga gaagggcatc tacatctacc
      121 agactttcag gcagtttgca gagaaaggtt acatcacggt cgaggggccg cttttgagcg
      181 tgtttcgtgc cgatgaggta aacctgacgc tagacagcct cgataccctg ccggttgctg
      241 aggcgcaggt tgtacaaaac agcgacgacg aagcgctgta tgtctattac ccgcgcctgg
      301 gctgttatgc ttcggtgcct gcttcggttt cacctgactt cgccagactg ctgagcgaag
      361 agggggagcg tattttgttc tgtggccggg ccatcgaagc gcagccaatc accgcctggt
      421 cataggggtg tgcacagctt cagcccctgc atctgttcag aatgagataa ttcgccgcgg
      481 ctgctgaggt gcagcgccgt aagctgttgt tttttcgcca gcgcgttgaa ttctgttgta
      541 cgagtaaaaa agagggcagt cgaccagcca tccgcgtgca tcgccgaggg gtggtaaacc
      601 aacgacagca cagtgtcgct ctcgctttct ttggtcagcg gattgatcag gtggccgcgt
      661 cttttgcccc gctcgtgcac ataccgcttg tcctgagcgg agacggaaat tgacccctct
      721 gaaattgtgc agctgaccag aacgcggttt gcctcttgcg gatcgtagac tgtcgtggta
      781 tgctctgtac cgtgaaccag caaatggccg ccaaagttga gcacgaatgc cccaatgccc
      841 agctttcgca aaacaacagc tacctgatcg agggcgtaac ctttggcaat accgccaaaa
      901 tcaagagcca tgcccttttt tttcaagccc gcgcgcgttc ctgaaatcag cacctggcgg
      961 tagtcgacca ggctgcctgc ttccaccagt tttacctgcg cagtatcaga ctcgccgcca
     1021 aaattttcgc gcataaggcc atacgctttg atgagcggca ggatcgtcgg gtcaaaccgc
     1081 ccctggcttt gggccgcgat cacgagggcc gtccgcagta cctcgagggt gcttgtgctt
     1141 aggggctgca gcagagacgc tgtaccagct gcggcattca gccgtgtgag ttcgctggta
     1201 tggtagacgc tcatgcggct gtcgatgtcg ctcaggacat ctgatattgc cgtaaaacca
     1261 acgtcgcact gctctggccg atcacaaatg agtgtagccg agagcagcgt acccattgag
     1321 ggcaggttgc gtgttatgga gttttttttt ccggcagtac gtcagaccgg ggcgtaagct
     1381 gaatttcgtc ggggaatact tcggtcttct tcagggtgct taaaggaagc agcaacagca
     1441 ggctgattac ggcaattgcc aatacccagc ttttcagctc agggcgcacc agcagcagat
     1501 gtatgatgag caagagcacg aaaagaaccg acaagtagaa atgaatccaa cgtgccgttt
     1561 cgagtactgg cggccatgga atcacctgca gagcgaaacc cgacagcgtc attgcagcca
     1621 cgaggccaat gagtgcgatg ccggtacgta agccctgccg aatttgcgcg acgagttgtg
     1681 gtatcacgtg gatactaaac agcatgccac agaggaacag ccaggtgaca acggcagcga
     1741 cgtgtgtctt gagcagcgcc tgtcgcagtg tcggaaaaaa atccgccggc tgcagcatga
     1801 aataaacaag cgcaagacct gtggtagtgc tgagccaggt cgtaccatag aaaaaaatcc
     1861 ggaagcgcga cgtgaacatc accgtttctg ctccagcact atcttatgca gcgccagcac
     1921 acgccttgcc gacgaggtga gcacactgca ggtcaatgtc gaacctgtga tggtcggaat
     1981 attgcctttg agggaaagtg caggcgaaag cgtctgcccg tggaacaggc tgagaaaggc
     2041 atcagaggcc atgtattcct gtggttcagc aaagctcagc tgttcaatat gcagaatacg
     2101 ttctttttca tcaatacaga tgagcacagt ttcgttcaga gttctgacac ggtgcgtatc
     2161 gagatatgca tagccgatca gcttgcctgc cttcgtgatt tcatagacga cgagaaacgc
     2221 cgtatcgatc ggctcatttg cgtactgcac agcgcgctta agctgctcag gggtcaggta
     2281 aaccgaccgg cgcttgagat tgtctgcctg cggaaaaacg gattgaatgg cttcagggaa
     2341 agtgcggtaa acgcgtgctt cggcctgcgt aatcaaaaga gtgagaacaa agatactttt
     2401 acgggcgagc atacaggcta cctatagtga gattcattct catttagaca gggtgtcaag
     2461 cagcctcgca tgtgaactgt gacgaccgga aaggtcatgc gacataatct ggaagccatg
     2521 gacttgcatg gcttccagat gggggacctt ttagaacagg tagcccagtc caaaattgag
     2581 ctggttaaac ccggtttggg ccttgttgct cgtgattgcg tagtcgatct ttgcaattat
     2641 ctggcgaata ggtttatagg caagaccgat cgtcaggaca ttttgctcgt agcgcgcgtc
     2701 gaagcgattg ccagccgcca ccttgtactg tgtatcaacc ttttcgagcc gaatgtgagg
     2761 gtagagttcc tgttctgtga tgttcagcag gcgaaacacg tcatacccga gttcgatata
     2821 agctccatac atctggtcag cggggccgat tgtggttgaa cccgaggctg tcctcagccg
     2881 atctgcccct gtcaaattag cgaaggtgac gaccgaacga aagtaaacgc cgcgaaagcg
     2941 gacgtcggcg tgcgcatcga gaatcgtggt ttcatgcata tgcaagtcag cggagccgct
     3001 cttgccgtaa tagcccgaaa cgccgaatat gctgcccgtg aagcctgtat agtcgacgcg
     3061 cagcactccc gagaactcat tggcaattgc ctcgttaccg cgttgccggc caccgcgcag
     3121 accctgtgag gtgaagttag gttgcttcgg ggtgccgttc gtcgggtctg tcatcgcatt
     3181 gaggctggtc acgccgtaga gtttataagc aaaatcgccc agctggccaa agataccggc
     3241 gccgttctcg cgccaggtac tcggcatgat gacggtttcc gtcagagggc gggtcgctgc
     3301 aagaaatgtc ggagcttcgt gcatctcgtt atagataccc atgggaatca gcagcagtcc
     3361 tgcgcgaaaa ttgagccgcg gatcgtgcag g
//
LOCUS       DAOQJF010000358         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18296_length_3359_cov_5.059019, whole genome shotgun sequence.
ACCESSION   DAOQJF010000358 DAOQJF010000000
VERSION     DAOQJF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18296_length_3359_cov_5.059019"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..702)
                     /locus_tag="PLF85_14905"
     CDS             complement(151..702)
                     /locus_tag="PLF85_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21009.1"
                     /translation="MKQKIYPFLICAAFSLHQPLAAEDLAERVRRSAVEMTEAFQRED
                     ADTLVRYTAQAVLDRTGGASVTAARIRAEFESNRKENIRLKKFSIIRPDRIYYLQGQL
                     FALVERKSEWQMEKETMNLRYNVLAVSTDGGATWRFAELTSIDEKLFRELFGFFDAEF
                     FGEFYKYTSERCNCAVTSGDKKK"
     gene            complement(782..1396)
                     /locus_tag="PLF85_14910"
     CDS             complement(782..1396)
                     /locus_tag="PLF85_14910"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE21010.1"
                     /translation="MPRELTEFYAPIPGKVPFFKGADMNPYWTNDGKALPPDLRRLSN
                     LSLTDSDGKALATADLSGKYVLVSFFFSRCSGICPMVTANVKRLREQLNDRRNVVFLS
                     ISVDPEHDKPEELRQFRARNKVPFADWHFTTGSKSEIYELARSVFQADVTVRGSKDAA
                     DFLHTESVYLLDTGLYLRGIYRTRGLPDLERLNRELGQLRTEGT"
     gene            complement(1447..>1839)
                     /locus_tag="PLF85_14915"
     CDS             complement(1447..>1839)
                     /locus_tag="PLF85_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21011.1"
                     /translation="GVNARQVTTYRNGLEHGPQRTWAENGMLVEERLFSEGKKTGVHR
                     GYFPDGTNRFYAEFKDDRYVNEFWSWHTNGKVAEFKRYDREGRALVYKHWRESGQIYR
                     NEVYAENLEQGMPGVKLCNSVKETTGKR"
     assembly_gap    1840..1939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1940..2171)
                     /locus_tag="PLF85_14920"
     CDS             complement(<1940..2171)
                     /locus_tag="PLF85_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21012.1"
                     /translation="MRAPWQNRIRSPLLLALLLVIGCRKQYQVTFDEKHLSISNGRLL
                     YEGTPLDGEVTREFGGVNARQVTTYRNGLEHGP"
     gene            complement(2195..3055)
                     /locus_tag="PLF85_14925"
                     /pseudo
     CDS             complement(2195..3055)
                     /locus_tag="PLF85_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
BASE COUNT          676 a          805 c          964 g          814 t
ORIGIN      
        1 agaatcttgt cgacgagagt tgttttgccg tggtcgacgt gggcaataat ggcgatgttt
       61 ttcatgtgtt gtctgcttct ttgcaccgcc aaaaagagaa aatttgatag ggtgtaaagt
      121 acaaccgggc ccggtgcgta cctggggggc ctatttcttt ttgtcgccgg acgtcacggc
      181 gcagttgcag cgttcgctgg tgtatttata gaactcacca aaaaattcag catcgaagaa
      241 gccaaagagc tcacggaaca gcttttcatc aatcgaggtg agctcggcaa aacgccaggt
      301 tgccccgcca tcggtagaga ctgccagcac attgtagcgc aggttcatgg tttctttttc
      361 catttgccac tcgctcttgc gctctaccag ggcaaaaagc tggccttgca ggtagtagat
      421 gcggtcaggc ctgatgatgg agaatttctt taacctgatg ttctctttcc ggttcgattc
      481 gaactccgcc ctgatgcgtg cggctgtaac cgaggccccg cctgtgcgat cgagaacagc
      541 ctgtgccgtg tagcgcacca gggtgtcggc gtcttcgcgc tggaacgcct cagtcatctc
      601 gactgccgaa cggcgtacac gctcagccag gtcttcggct gccagaggtt ggtgcagaga
      661 gaacgcggca cagatcagga agggataaat cttttgtttc acgggccatg gcctgtttgt
      721 gcgcccgggt gaaaatagtt cttacaggct cctggaaaag gaacctgcta atggaaaggg
      781 cttatgttcc ttcggttctg agctgcccga gctcacggtt caggcgttca aggtcgggca
      841 ggccccgcgt acggtagatg ccgcgcaggt agagacccgt gtcgagcagg tagaccgatt
      901 cggtatgcag aaaatccgcc gcatcttttg agccgcgtac agtgacgtcg gcctggaaaa
      961 cggaacgggc aagctcataa atttcgcttt tgctgcctgt agtaaagtgc cagtctgcga
     1021 acggaacctt gttgcgcgca cgaaactggc gcagttcttc gggtttgtcg tgttcagggt
     1081 caacactgat agaaaggaaa acgacattgc gccggtcatt cagctgctcg cgcaggcgtt
     1141 tcacatttgc cgtaaccatt gggcaaatac ccgaacagcg gctgaagaaa aaagaaacaa
     1201 gaacgtactt gccgctgaga tcggcagttg ccagcgcttt gccatcgctg tcggtgagcg
     1261 ataggttcga caggcggcgc aggtctggcg gcagtgcctt accgtcgttc gtccagtaag
     1321 gattcatgtc tgcaccttta aaaaacggaa ctttgccggg tatcggtgcg tagaattcgg
     1381 taagttcgcg cggcatctcg ggcctcttgt tgcccccgca ggcagcagca agaagcagca
     1441 gggcgatcag cgtttgccgg ttgtttcttt tacactgttg cacaatttta ctccgggcat
     1501 tccctgttcg agattttcgg catagacctc gttgcggtat atttgtcctg attcacgcca
     1561 gtgcttatac accagcgcac ggccttctct gtcgtaacgc ttgaactccg ccaccttgcc
     1621 gttggtatgc cacgaccaga attcattcac gtagcggtcg tctttgaatt cagcgtaaaa
     1681 acggtttgtg ccgtcgggaa aatagccgcg gtgcacaccg gtttttttgc cttcgctgaa
     1741 gagccgctct tcgaccagca taccgttttc agcccaggtg cgttgcgggc catgttccag
     1801 cccattgcgg taggtcgtaa cctgcctggc gttcacgccn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnng cgggccatgt tccagcccat tgcggtaggt cgtaacctgc
     1981 ctggcgttca cgccaccgaa ttcgcgcgtg acttcgccat caagcggcgt accctcgtag
     2041 agcaggcggc cgttgctgat agacaggtgc ttttcgtcga aagtaacctg gtactgttta
     2101 cggcagccaa taacaagaag cagcgcgagc agcaaggggg agcgtatgcg gttctgccac
     2161 ggcgcgcgca tggtgtcttt ttgcctttct tctgtcagtt tgataccgaa ccgatggtac
     2221 cgcggaaata cgaacccatc agcgttttga tcgtggcagt cggatcctgc gcatagtggt
     2281 agtggtaggt ggcggtggtc atgtgccggg ttttggtcgt atggccgtga agcttatcga
     2341 gcgaggttgc gctgtcgccg ttgccggcac cggtatcgtt tgccgaattg cctgtcgctg
     2401 cagctgtcgg ggcagtgagg gttacatcgt cgctggtgtc agcggtgcca ttgtcgcatt
     2461 tctcggcata gatcaggtag ccgtcgaggg ccacaccgat gagggttgag tcattcatgt
     2521 tcgaagacaa cttgttcaca gctgcatggt ggtgaaagac gccttggttt tgcgggtggc
     2581 caccgtagtt atcaaatgta gcggcttcaa cagcaagcgt gtcgggtggg gccgccgcgt
     2641 tgttaaagac cgtgatgcca ttggcggtga tgccaatcgc gacgagacca ccctgcgagc
     2701 ttgtgagcgt acctgatttt agggtgggtg tctgcggtat ggtaaaggtc aggttttgtg
     2761 tcgagatgac attggttcct gcagagatgt tcgtacctgg cagcgcctcc caaagggtcg
     2821 cattctggcc ggtggtgcag gtcgttgaac ccgcgccatg tgaaccgcag taatagaagc
     2881 tgttatggtt cggcaggtta accgacttaa agatgtaatt agtaccagag gtataggcaa
     2941 cctggcattt gaagttgtct ttagcccatg agggcatggc ggagtctatc gtcgtattca
     3001 cgttcgttat gcagccggtg gtagcattga gtgttgcggt ggtcgggctg attgtggcgc
     3061 tcgttgtcgg gtaggatgtc gaactagagc tggtcgtcgt tgtcgatgcc tctgtagtag
     3121 agtcgctgtt cccggcacag gcggtcagta cgccagcgat aaaaattgca aaaatcggat
     3181 acttgggttt tttcatgggt tactcctgaa cagtagatca tcctataaga caaaccggac
     3241 ataaaaaaag tgacccatga gcaaaagtac tggaggcggt atgcaaagtc tggccactgg
     3301 acttccgctt ctgcggtaat tttgagtgag aggttatcat gaaggaaaaa attctatcc
//
LOCUS       DAOQJF010000359         3350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18390_length_3350_cov_4.246131, whole genome shotgun sequence.
ACCESSION   DAOQJF010000359 DAOQJF010000000
VERSION     DAOQJF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18390_length_3350_cov_4.246131"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PLF85_14930"
     CDS             complement(<1..1403)
                     /locus_tag="PLF85_14930"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE21013.1"
                     /translation="MRFILISLLLVSSTALAAASDPAPLVTGKQSLGRWLEFRRETVN
                     GQSITEIAKRAFETNLTGKEAINFGYTKDHIWVRFSVTNVTAAGLWYLRLGTLVDKAS
                     LYEEESPGKWRERKLGRFEPFSQRELTHRDLVFVLSIAPQTTKTFYLEIASAGNVTVQ
                     LDITDARTFAAADHDAQFAFGLYFGLMLIMVGYNLFLFLSTRELTYLWYVSYVLFFTL
                     LHLSINGYVAEYLWPRHLPVLPFLTPLFISVSGVFSVLFTRQILGLRGLRGHMETLSG
                     ILLGLFLIAVLAAPLAPYTVAMRAATYSTLIAILGLTYCGTRRLLQGQKSARFFLLGW
                     LALYTGIAAMLLRNLGILPHNFFTAYAMQIFSAAEVTLFSLALGDRLNQLREDSLKLQ
                     QVRKELDIARKMQLALVPPVVLTAPRVDIAHRYIPMLDVGGDFMDIRITEEGISMFIG
                     DVSGHGIGAAMIATTMKL"
     gene            1512..2612
                     /gene="aroC"
                     /locus_tag="PLF85_14935"
     CDS             1512..2612
                     /gene="aroC"
                     /locus_tag="PLF85_14935"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNE21014.1"
                     /translation="MGSSFGRLFRVTTFGESHGEGLGCVVENCPAGLALTEADIQPDM
                     DRRRPGQSRITTPRDEPDRVKILSGTFEDKTIGAPIAMAVFNENAREKDYSDIKEIFR
                     PSHADYTYHARYGHRAWRGGGRSSARATIGIVAAGAVAKKILREKAGVEIVAYVSQIK
                     DLRFEGELPLADAGKLREMIESNPVRVPDAALAEKMYRLIQDTRQAGDSLGGIVECAA
                     IGVPAGWGDPVFDRLDALIAQAMLAVPATKGIEIGSGFSAVTMTGKEHNDIFEAHGGK
                     ITTRTNHSGGIQGGISNGMPITFRVAFKPTATISHEQPSVNERGENVTLKVKGRHDPC
                     VLPRAVPIVEAMLALVLVDQFMISKTARFDSL"
     gene            complement(2614..3201)
                     /locus_tag="PLF85_14940"
     CDS             complement(2614..3201)
                     /locus_tag="PLF85_14940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE21015.1"
                     /translation="MKLLEAKASLSFQKTLSQDKVYEFFNSLTLIDPDLRLPNTVYLN
                     LYLTQEIARMRFTQFDIAVLAVGVDEMNKFEHIYGAPAAMAGLRAVAEVVADYPKWPR
                     GMTFRYGRDRLVVVLPQKNREQAVDTAEKIKDAIHQRFQKNREKLTAEMTVSFGITAM
                     PASTGATDRLLVKAVEALDLGQRQGKSKIKYADFT"
BASE COUNT          769 a          899 c          951 g          731 t
ORIGIN      
        1 agcttcatcg tggtagcgat cattgcagcg ccgataccat gtccgctgac atcgccgatg
       61 aacatcgaga ttccttcttc ggtgatgcgg atatccatga agtcgccgcc gacatcgagc
      121 atcggaatat aacggtgcgc tatgtcaacg cgcggtgcag ttagcacgac cggcggtacg
      181 agcgccagct gcattttacg cgcaatgtcg agttctttgc gcacctgctg cagctttaga
      241 gaatcttcgc gcagctggtt taacctgtca ccgagtgcca gcgaaaacag ggtaacctcg
      301 gcagcggaaa atatctgcat cgcataggcg gtaaaaaaat tgtgcggcaa gatgccgaga
      361 ttacgcaaaa gcatcgctgc aatgccggta taaagcgcca gccagccgag caggaaaaaa
      421 cgcgcgctct tttgcccctg caacaaccgc ctcgtgccac aataggtaag cccgagtatc
      481 gcaatcaggg tgctgtacgt tgcggcccgc atcgcaacgg tgtaaggggc aagtggcgca
      541 gccaacacgg cgatgagaaa gaggcctagc aggatacctg acaaggtttc catatgtccg
      601 cgcaaaccgc gcaggccaag tatctgcctg gtgaagagta ccgaaaagac tcccgatacc
      661 gagataaaga gtggtgtcaa aaatggcagt accggtaaat gccgcggcca gagatattcg
      721 gcaacatagc cgttgatact caggtggagc agtgtaaaga acagcacata gctcacgtac
      781 caaagatagg tcagctcgcg tgtcgacagg aacaggaaca ggttgtaacc gaccatgatc
      841 agcatcaacc caaagtaaag accaaaagcg aattgtgcgt catggtctgc tgcggcaaac
      901 gttcgtgcgt cggtgatgtc gagctgtacc gtgacgttgc ccgcgctcgc gatttcgaga
      961 tagaacgttt tggtcgtctg cggtgcgatc gaaagtacaa ataccaggtc tctgtgggtc
     1021 agctcacgct ggctgaaggg ctcaaaccgg cccagtttcc gttcccgcca ctttcccggc
     1081 gattcctctt cgtacaggct ggccttatcc accagcgtgc cgagtcgcag gtaccaaaga
     1141 cctgcagcgg ttacgttggt gacggaaaaa cgcacccaga tatggtcctt cgtgtagcca
     1201 aagttaatgg cttcttttcc ggtcaggttg gtttcaaagg cgcgttttgc aatttcggtt
     1261 atgctttggc catttaccgt ttcccgccgg aattccagcc aacggcccag gctttgtttt
     1321 ccggtaacca agggggccgg atcactggcg gcagcgagcg ctgtcgatga aacaagcaaa
     1381 aggctaatca gtataaatcg catcgcacga accgggtcga tcgccctgat aggcaatacg
     1441 tgcaggttgg caaagcgatt tttctctgcg gggattgttt tacagcctct ctctgataac
     1501 cattaaagcc tatgggctct tcattcgggc ggcttttccg cgtgactact tttggcgaat
     1561 cgcatggcga aggactcggt tgtgtggtcg aaaactgccc tgcggggctc gcgctcaccg
     1621 aggccgacat ccagcccgac atggaccgcc ggcgtccggg ccagagccgc atcacaaccc
     1681 cccgcgacga gcccgaccgg gtgaagattc tttcgggtac cttcgaagac aagaccatcg
     1741 gtgccccgat cgcgatggcg gtcttcaacg agaatgcgcg cgagaaagac tacagcgata
     1801 ttaaagagat ctttcgcccg agccacgcgg actataccta ccacgcgcgc tacggtcacc
     1861 gggcctggcg gggtgggggg cgttcatcgg cgcgcgcgac gatcggtatc gttgctgcag
     1921 gtgcggtggc aaaaaaaatc ctgcgcgaaa aagccggcgt agaaatcgtt gcatacgtct
     1981 cgcagatcaa agacctgagg tttgaggggg agctccccct tgctgatgcc ggcaaactca
     2041 gggagatgat tgaatcaaac ccggtgcgcg tgcccgacgc ggcgcttgcg gagaaaatgt
     2101 accgcctgat acaggatacc cggcaggcag gtgactcgct cggtggtatc gttgaatgtg
     2161 ccgcaatcgg tgtgccggcg ggttggggtg acccggtgtt tgaccgcctt gacgcgctga
     2221 ttgcgcaggc gatgctcgca gtacctgcga ccaagggcat cgagatcggc agcggatttt
     2281 ctgcggtgac gatgaccggc aaagaacaca acgatatctt cgaagctcac ggcggaaaaa
     2341 tcacgacgcg gactaaccat tcgggcggta tacagggcgg catttcaaac ggcatgccga
     2401 tcacgtttcg tgtcgcgttc aaaccgaccg cgaccattag ccacgagcaa ccttcagtca
     2461 atgagcgcgg cgaaaatgtt acccttaagg tgaagggcag gcatgatccc tgcgtattgc
     2521 cgcgcgcggt gccgatcgta gaggcgatgc ttgcgctcgt gctcgtcgac cagttcatga
     2581 tttcaaaaac cgcgcgcttt gattctcttt gagttaggta aaatcagcgt acttgatctt
     2641 gctctttccc tggcgctggc ccaagtctag agcttcgact gccttaacca aaagacggtc
     2701 ggttgcgcct gttgaagccg gcatcgcggt gatgccaaac gagacggtca tttctgcggt
     2761 cagtttttcc cggtttttct gaaacctctg gtgaatcgca tctttgattt tttcagcagt
     2821 atcgactgcc tgttcgcggt ttttctgggg taatacgacg accaggcggt cgcgaccata
     2881 gcgaaaagtc atgccgcgcg gccattttgg gtaatcggca acgacttctg caaccgcgcg
     2941 caggccggcc attgccgccg gcgctccgta aatgtgttca aatttattca tttcatcaac
     3001 gcctacggcg agaaccgcaa tatcgaactg ggtaaaacgc atgcgggcaa tttcctgcgt
     3061 caggtacagg ttcaggtata cggtattggg caggcgcagg tcggggtcaa ttaaggtgag
     3121 gctattaaaa aactcgtata ccttatcctg actcaaggtc ttctggaaag aaagagatgc
     3181 cttggcttca agaagtttca cggctttttc aagctccaaa ttccgaagtt ccgtcctggc
     3241 ccaagccgca agattttgta gaattgccgt ctcgcgttcg gtaagctgtc ggggttggcg
     3301 atcgattacg cacatggtac ctatgcgcag tccgttctcc atttcaatcg
//
LOCUS       DAOQJF010000360         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18463_length_3343_cov_5.129866, whole genome shotgun sequence.
ACCESSION   DAOQJF010000360 DAOQJF010000000
VERSION     DAOQJF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18463_length_3343_cov_5.129866"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PLF85_14945"
     CDS             <1..1231
                     /locus_tag="PLF85_14945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21016.1"
                     /translation="NMATNADSPNFELKLRGSGYGAVLLKDIRAGAGNSSPYAFISYQ
                     GFVYFAATDGINGFQLWRTDGTPEGTTLFKNFSPGGNLHATDHSMTVLNGLLYLRGED
                     TTHGDELWKTDGTVAGTVFLADIYSVSNNDADPTFLTTLGSELIFRARRNTTESYEVF
                     KSNGNAGNFTFVKDINPTASTSSNASYFTPMGGNAYFQANDGTNGAELWKTDGTTGGT
                     VMVADLYAGATGSSPYNLVALGGFLYFCAETSAQGFELRRTDGTTISLVIDLWPGGND
                     GCHSTNLGQIGLWNSTLLFSGSNGSGVTSELYKSDGSIGNATLVKDINPTGFSAPTGF
                     VQLGNYTYFSATDGSNGVELWRTDTTAAGTTLASNIDLTNASSSPTGMTVLGDSLIFS
                     ATTTALGTELYIYLPPP"
     gene            complement(1389..1943)
                     /locus_tag="PLF85_14950"
     CDS             complement(1389..1943)
                     /locus_tag="PLF85_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21017.1"
                     /translation="MTHGRVAAQDELLGVGRSSLRRKILLALCHTCLEYVDFLFVTIP
                     LARHFQTGFFCTLSQPGFVGVILPLATIFGIILAVTPPPVNITRTPNHIVTRIGLYSR
                     LGAIDCIGIRCGTLALLITLEPQCFFCASVECRFFQPVTFFCPQRGIIFGALNAQVFV
                     RVEWTTRYFYPVYSQRIAFAFVPF"
     gene            2021..2518
                     /locus_tag="PLF85_14955"
     CDS             2021..2518
                     /locus_tag="PLF85_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21018.1"
                     /translation="MTKNVINFLVGCLAVMSAASIYANKAKCESESFARQKTAEKAAQ
                     TEYDNWPGHATYKHLYANFEKARDRYNEVEKRFIEVNNTHIRALQECSKIRNKDENDA
                     CVSAALKPLNAAREERDAKKKLQENAARREVEFRPQNVAAREKMDAAKQAAYKAGAEE
                     RRKCK"
     gene            2673..>3343
                     /locus_tag="PLF85_14960"
     CDS             2673..>3343
                     /locus_tag="PLF85_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21019.1"
                     /translation="MYSTVLRFLLIFAFALSAQGLFAQSLKKVMILDFKNLDKNPDYS
                     YLEESITEAIRNELKAKFDFREFPQKDWRNLAEKNFFLWPEENHSRGFALNLGIMARQ
                     DIAVGGYYQAIIEKKSRKGVAAGSYVIRAHVYVLDVGKRKVVSEFDMVMPADASLFGA
                     VEELAARVVKEAKSVLPNKGDAGKQQLDDGDMGPGEFGLIAGTGVYALPAQFSGNYTS
                     DVTLYA"
BASE COUNT          897 a          885 c          895 g          666 t
ORIGIN      
        1 caacatggcg acgaatgccg atagcccgaa ctttgagctg aaactgcgcg gctcggggta
       61 cggggctgtt ctgctgaagg acatccgggc aggcgcggga aattcgtctc cctacgcatt
      121 tatcagctac caggggttcg tctattttgc ggccaccgac ggcatcaatg gcttccagct
      181 ctggcgcacc gatggcacac ccgagggcac gaccctattc aagaatttta gcccaggggg
      241 aaatttacac gccacagacc acagcatgac cgttctcaat ggtctcctct acctcagagg
      301 tgaagacacc acacacggtg acgaactctg gaaaaccgat ggtaccgtgg ccggcacggt
      361 ctttcttgcg gatatctatt ctgtttccaa taatgatgct gatcctactt tccttacgac
      421 gctcggaagt gagttgattt ttcgggcgag gcggaatacg actgagtcgt atgaggtctt
      481 taaaagcaac ggtaatgccg gcaattttac gttcgtaaag gacattaacc ccaccgccag
      541 cacaagcagt aatgcatcgt acttcactcc catgggtggc aatgcgtatt ttcaggccaa
      601 cgatggtacc aatggcgccg aactctggaa aaccgacggt accaccggcg gcaccgtgat
      661 ggtagcggat ttatatgccg gggcgactgg atcttcgcca tataatctgg ttgcacttgg
      721 tggcttcctc tatttctgcg ccgaaacctc cgcccagggc tttgagctca ggagaaccga
      781 cgggacaacg atttcgttgg tcatcgatct gtggccaggc ggtaacgacg gctgccatag
      841 caccaatctt ggacagatcg gtctgtggaa ttcaacgctg ctattctcag gctcaaatgg
      901 gagtggagtt acctctgaac tctacaaaag tgatggaagt attgggaatg caacattggt
      961 caaggacatc aacccgaccg ggttttcggc gcccacgggt ttcgttcagc tgggaaatta
     1021 cacgtatttt tctgccaccg acggctccaa cggcgttgag ctctggcgca ccgataccac
     1081 cgcagcgggg acgactcttg cgagcaacat tgatctgacc aatgcatcct catcacccac
     1141 aggtatgaca gtcctgggcg atagccttat cttctcggct acgacgacag ctctgggcac
     1201 tgaactctat atctacctgc cgccaccgta agccaatgaa actcaacccg ctgctgctat
     1261 acacccattg cctgctcgcc acggcgtcgc tgctaccacg ggatgtacca gcagcgcccc
     1321 ctgactgccg caaagccgcc ggcgcgcaac gcaacacagc caatagcgca tgcgaaaacc
     1381 gacccgagtc agaaaggtac gaatgcaaac gcgatgcgtt gagaatatac cgggtagaaa
     1441 tagcgcgttg tccactcgac ccgaacaaag acctgtgcgt tcaacgcgcc gaaaataatg
     1501 ccgcgctgcg gacagaagaa tgttaccggc tggaagaaac ggcactcaac cgatgcacaa
     1561 aagaagcact gcggctcgag cgtaatgaga agagccagtg tcccgcaccg gatacctata
     1621 cagtcaatgg cgcccaaccg agaatacaaa cctattcttg ttacaatgtg attcggcgtg
     1681 cgcgtgatgt tgacaggcgg cggtgtgacc gcaagaataa tcccgaaaat tgtcgccaac
     1741 ggcaagatga cacctacaaa gcccggctgg gacagtgtgc aaaaaaagcc cgtctggaag
     1801 tggcgcgcga gcggaattgt tacgaacagg aaatcaacat attcgaggca ggtatggcaa
     1861 agtgccaaca ggatttttcg ccgcaaggaa ctacgcccga cgccgagcag ttcgtcctgt
     1921 gcagcgacgc ggccgtgagt cacctggcca gtcgcagaat gagctgtgcg aacatcagcg
     1981 aagcagaata caaggaaaat tacaggaagg agaataaata atgacaaaga acgtgatcaa
     2041 ctttttagtc ggctgtctcg cagtgatgag cgctgcgagc atatacgcca acaaggcgaa
     2101 gtgtgaaagc gaatcattcg cccgccagaa aacagccgaa aaggcagctc agacggaata
     2161 cgacaactgg ccgggtcacg caacgtacaa acatttgtat gccaatttcg aaaaggcacg
     2221 ggacagatac aatgaggtcg aaaagaggtt tatagaggta aacaataccc atatccgggc
     2281 gctacaagaa tgctcaaaaa tacgcaacaa agacgaaaat gatgcgtgcg tatcagccgc
     2341 actcaaacca ctgaatgcgg cgcgcgagga aagagatgca aagaagaagc tacaggaaaa
     2401 tgctgcccga cgagaagtgg aattcagacc acaaaatgtt gcggccaggg aaaaaatgga
     2461 tgccgcaaaa caggccgctt acaaggccgg agcggaagag cgccggaaat gcaagtaagc
     2521 gaatcatagc aacgcacggc attaatctca gcagaagggc acaatatgag cctgggcttc
     2581 cagcaaacca accccataac cgagaatcac tctcaaaaat gggatatttt tcctttacat
     2641 gataaccata cccggctacg cttgtagtcc gcatgtactc tactgtactc cgatttttgc
     2701 tcattttcgc cttcgctctg agcgcacaag gactgttcgc ccaatcgctc aagaaggtga
     2761 tgatccttga tttcaaaaac cttgataaga acccggacta cagctatctt gaagagtcga
     2821 ttactgaagc gattcgcaat gagctgaagg cgaagtttga cttcagggag tttccgcaga
     2881 aggactggcg caacctcgcc gagaaaaatt tctttctttg gcccgaagag aaccacagcc
     2941 gtggttttgc gctcaatctc ggcatcatgg cacggcagga tatcgcggtc ggcggctatt
     3001 accaggcgat tatcgagaaa aagtcgcgca agggcgttgc agccggcagc tacgtcattc
     3061 gtgcgcatgt atacgttctc gatgtcggta agcgcaaggt cgtgagtgaa ttcgacatgg
     3121 tgatgcctgc cgatgcctcg ctctttggtg cggtcgaaga actcgccgcg cgcgtggtga
     3181 aagaagcgaa gtccgtgctg cctaataagg gagatgccgg caagcagcag ctcgacgacg
     3241 gcgacatggg gccgggggag ttcgggctca tcgcgggtac aggcgtctat gcgctgcctg
     3301 cacagttcag cggcaactat accagcgacg ttactctcta tgc
//
LOCUS       DAOQJF010000361         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18512_length_3337_cov_4.528641, whole genome shotgun sequence.
ACCESSION   DAOQJF010000361 DAOQJF010000000
VERSION     DAOQJF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18512_length_3337_cov_4.528641"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1985)
                     /locus_tag="PLF85_14965"
     CDS             complement(15..1985)
                     /locus_tag="PLF85_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE21020.1"
                     /translation="MVSHIYFDPALSRITLESFRDPVLFATIQDNLAIKPSHPDEEHA
                     VVWLLSSAALTENAQLVSNWLQKYPQHRSRLVVLGDHEFSELLFAPLREDQIHLYLPA
                     AMPMAQVRRACETQINALHAEYDRLQLQEKLANSYIEIRRLTAVGQALATERDFDRLI
                     ELVLNSAIELTAADGGSLYLAEKHGERDKPTHIRFKKSAMQLDADEFLLPINKKSIAG
                     YVALTAEPLLIDDVHNLPVDAGYTYNSEYDKAHNYYTKSMMVIPMKNHHDEVIGIIQL
                     INRKRDPEMKPTLADLKGDAVVSFTRKDYSLVSAMAGQAAVAIENNQLLQDIQNLFEG
                     FVKASVTAIEQRDPTTSGHSFRVADYSVNIALAVNRDTGPRFNATRFNRNQIQELRYA
                     SLLHDFGKVGVREDVLVKAKKLYPAQLELIRWRFHYIRKDIEAGMLRQSLAEMQKSPR
                     ELWPEIQAKFQAEQEKQQLEAEQMFSAILSANEPTVLAAGSFDFLKKIAENKLRVSVD
                     EELPFLTDQELVMLSIRKGTLDERERLEIESHVTHTYNFLRQIPWTPNLRWVPEIAYG
                     HHEKLNGGGYPLKRRDPEISLQTRMMTIADIYDALTAWDRPYKKAIPPEKALDILHME
                     VKDGHVDPDLLQVFVQAKIFERIEELRALGAG"
     gene            2122..2547
                     /locus_tag="PLF85_14970"
     CDS             2122..2547
                     /locus_tag="PLF85_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNE21021.1"
                     /translation="MLFERLQKESLEARKARDTVRAGVLTTAISQVKALAIDDGHRAP
                     NDADVLKVVRQFLKSCEENLALAAQGKMDASRAEQFKVEKDVLLSFLPQQMSAEDLKA
                     AITKSGATNIGEVMKYLKANHDGQYDGKLASQVAKEVIG"
     gene            2544..>3337
                     /locus_tag="PLF85_14975"
     CDS             2544..>3337
                     /locus_tag="PLF85_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21022.1"
                     /translation="MMYIWHAFKHKLNLLFLGAGLVAGVFFSWSLPLFYPVLFAAEAV
                     ILIGTATNKRFQRAVNALEGREAEKDEIRRLRNAIYGSRSALKSKMSPAEELIRQIRQ
                     NTRQNSKASVQSSEAMLTGLDELEANFIHLLNGYCRLDDMQKTAGTGHDAEVAKLKSD
                     LAALAADAPAETRDALTRRVELAEKRASIVKDAEQNKQKLLAQIEMVEDTLKYLRDQS
                     VQVFDANVIAKQVTAISEKMQTTRETMLEIEQMSAGTDYTLQQTRIR"
BASE COUNT          735 a          929 c          957 g          716 t
ORIGIN      
        1 ggtgaactga actcttagcc tgcgccgagt gcgcgcaact cttcgatgcg ctcgaaaatt
       61 ttggcctgca cgaaaacctg cagcaggtct gggtcgacgt gcccgtcctt gacttccatg
      121 tgcagaatat cgagcgcctt ctcgggcggt atcgccttct tgtaggggcg atcccaggcg
      181 gtgagcgcat cgtagatgtc ggcaatcgtc atcatgcgcg tctgtagcga aatttccggg
      241 tcacgccgtt tcagcgggta gcccccaccg ttgagttttt cgtggtggcc gtacgcgatt
      301 tcgggtaccc agcgcaggtt gggcgtccac ggaatctgcc gcaggaagtt gtacgtgtgc
      361 gtgacgtgag attcgatctc gagccgctcg cgctcatcga gcgtaccttt gcgtatcgac
      421 agcatcacga gttcctggtc ggtaagaaaa ggtagctcct catcaaccga gacgcgcaac
      481 ttgttttctg caattttttt gagaaaatcg aatgaaccgg cagcgagcac ggtcggctca
      541 ttagccgaaa gaatagccga gaacatctgc tcggcctcga gctgctgctt ttcctgctca
      601 gcctggaatt ttgcctgtat ctcgggccac agctcgcggg gggatttctg catttctgca
      661 aggctctgcc gcagcatgcc agcttcgata tctttcctga tataatggaa acgccagcgg
      721 atcagctcaa gctgcgcagg gtagagcttc ttcgccttga cgagcacgtc ttcgcgtacc
      781 ccgaccttgc cgaagtcgtg cagcagcgaa gcataacgca gctcttgtat ctggttgcgg
      841 ttgaagcggg tcgcattaaa gcgcggcccg gtatcgcggt tcaccgcgag cgcgatattc
      901 actgaatagt ctgctaccct gaacgagtga ccgctggtgg tcgggtcacg ctgttcgatg
      961 gccgttaccg atgccttgac aaacccttca aacaggttct ggatatcctg cagcagctgg
     1021 ttattctcga tcgccacggc cgcctggccg gccatcgcgc tgaccagcga atagtctttg
     1081 cgggtaaacg aaaccaccgc gtcgcctttc aggtcggcga gcgtgggctt catctcgggg
     1141 tcgcgtttgc ggttgatcag ctgtataatg ccgatcactt cgtcgtggtg gttcttcatc
     1201 ggaatcacca tcatcgactt ggtgtagtag ttgtgcgcct tgtcgtattc cgagttgtac
     1261 gtataacccg cgtcgaccgg caggttgtgc acgtcgtcga tgagcagcgg ctcggcggtc
     1321 agcgccacat aaccggcgat ggattttttg ttgatcggca gcaagaactc gtcggcgtca
     1381 agctgcatcg ccgatttctt gaaacggatg tgtgtcggtt tatcgcgttc gccgtgtttt
     1441 tccgcaaggt agagcgagcc gccatcggcg gcagtaagtt caattgccga attcagtacc
     1501 agctcaatca ggcggtcaaa atccctttcg gtcgcgagcg cctggcccac tgcggtcagc
     1561 cggcgtattt caatgtagct gtttgccagt ttttcctgca gctgcaggcg gtcatactcg
     1621 gcatgcagcg cgttgatctg cgtctcgcac gcgcggcgca cctgcgccat tggcatcgcg
     1681 gcgggcaggt acaggtgaat ctggtcttcc cttagcgggg caaacagcag ttcagaaaac
     1741 tcgtggtcgc cgagcacgac gaggcgcgaa cggtgctgtg ggtacttctg caaccagttg
     1801 gacaccagct gcgcattttc ggtgagcgcg gcggaactca acagccagac gacagcatgc
     1861 tcttcgtcgg ggtggctcgg cttgatcgca agattatcct gtatggtggc aaaaagcaca
     1921 ggatcccgaa atgactccag cgtgattcgc gaaagagcag ggtcaaagta gatatgcgag
     1981 accacggttc atggcagtta gcggtaaacg ttcggtcaag gataatagac cccaaattgt
     2041 ccggtaaaga tgtgcaacag acaggcgccg ggcctgctct cataacactt gacatcggcc
     2101 atagccgaca cgcatttccc catgcttttt gaacgcttac agaaagagag ccttgaagcc
     2161 cgcaaggcgc gcgataccgt gcgcgcaggg gtactcacta ccgccatttc gcaggtgaag
     2221 gcactcgcca tagacgacgg ccaccgggca cctaacgacg ccgatgtact caaagtcgtc
     2281 cgccagtttc tcaagtcatg cgaagaaaac ctggcgctcg ccgcgcaggg taaaatggac
     2341 gcctcgcgcg ccgagcagtt caaggtcgaa aaagacgtct tgctttcatt tctgccgcag
     2401 cagatgagcg ccgaagacct gaaggcagca attaccaaat cgggcgccac gaacattggc
     2461 gaagtcatga aatacctgaa agcgaaccac gacggccagt acgatggtaa actggcatcg
     2521 caggtcgcaa aggaggtcat cggatgatgt atatctggca tgcatttaag cataaactca
     2581 acctgctctt tttgggagct ggactggtcg cgggcgtttt tttcagctgg agcctgcctc
     2641 ttttttaccc ggtcctcttt gccgccgagg cggtgatcct catcggtaca gcgaccaaca
     2701 aacgtttcca gcgggcagtc aatgcgctcg aggggcgtga agcagaaaaa gatgaaatcc
     2761 gccgcctgcg caatgctatc tacggttcgc gctcagctct gaaatcgaag atgtcgcctg
     2821 ccgaagaact gatacggcag atcaggcaga acacccggca gaacagcaag gcgtcggtgc
     2881 agtcgagcga agctatgctg actggactcg atgagctcga agcaaatttc attcacctgc
     2941 tcaacggcta ttgccgcctc gacgacatgc agaagactgc cggcaccggc cacgatgccg
     3001 aagtcgcgaa actcaaaagt gaccttgcag cgctggcagc cgacgccccc gccgagacac
     3061 gcgacgcttt gacccgccgg gtagagctcg cagagaagcg tgcctcaatc gtgaaagacg
     3121 cagagcagaa taaacagaag ctgttggcac agattgaaat ggtcgaagac acgctcaagt
     3181 atctgcgcga ccagtcggta caggtattcg acgccaacgt catcgcgaaa caggtgactg
     3241 ctatctcaga gaagatgcag acgacgcgcg aaaccatgct cgaaatcgag cagatgtctg
     3301 ccggtacgga ttacaccctg cagcagacgc gaattcg
//
LOCUS       DAOQJF010000362         3329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18597_length_3329_cov_3.842700, whole genome shotgun sequence.
ACCESSION   DAOQJF010000362 DAOQJF010000000
VERSION     DAOQJF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18597_length_3329_cov_3.842700"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLF85_14980"
     CDS             <1..539
                     /locus_tag="PLF85_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE21023.1"
                     /translation="NGAGKSMLFRALLGQCPLTVTGSVQVHYKRLSLFDQDQTSIAPQ
                     TSVLSLARENFPARDEGEVRRLLGAFGYGGSAVFRDFRQLSTGERVRLLVLLLSKSDN
                     FPDLLLADEAETGLDRETRALFARFLHEYPGLLLFTTHSEEFAAAVKPSRSITLTRPN
                     NFSAEPATAAHAALHPRE"
     gene            complement(487..2622)
                     /locus_tag="PLF85_14985"
     CDS             complement(487..2622)
                     /locus_tag="PLF85_14985"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNE21024.1"
                     /translation="MHRGIFVLGLLFLVHCGRQQVTVPTELSGGLKAGDLLLEYQPAG
                     QYLRLMDVRLNRVVLRLPLAPGFLSARVATDAVRERLASYKMHEVVREHCELQRISAG
                     EASTAAINLAAELSCSGTPAAIEISFTQAADHVALKIRPRDARFNRLLFSFMAEEGER
                     VFGGGEQFTHLDLRGRRIPMLSEEQGIGRGAQPITIGANLTAGAGGRDVTTYAPVPFF
                     MTSAFRSFETSQTAYQLWDFKSDHYCVEIWDNYLELRFSTAFAYGELMKRYTARSGRM
                     QPLPEWASGTILGVQGGKDKVEQVLNAALKAGNPVTAIWIQDWVGRRLTGFGSQLWWR
                     WLPDETAYPNFRAWVQQLRARGVRVLGYINPFLADEGPIFAEAKAKGYLVRKRDGEPY
                     KIQTAGFPAYLVDLSNAEARTFLKNIIRNNLIGNGLAGYMADFGEWLPWDAQLASGES
                     AAHWHNRYPVEWARLNREAIREAGLEREVVFFTRAGFAGSAEHSTLFWLGDQMVSFDE
                     YDGLASAVTGLLTGGISGITLNHSDIGGYTTINNPVKNYHRSRELFLRWAEFAVFTPF
                     FRTHEGNRPEKNYQAYSDEKIVAEFAALGRQHLALKPYFAQLMAEAAKSGMPLARPVF
                     LHYPAEQETYLLRHQFLLGRDLMVAPVIEPAATSGRVYLPADTWIESSTGKKVTGPQW
                     HTFAAPLGQPVAFIREGAAQRELLLQALR"
     gene            2712..>3329
                     /locus_tag="PLF85_14990"
     CDS             2712..>3329
                     /locus_tag="PLF85_14990"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNE21025.1"
                     /translation="MNDIIARKKAGQKLTKQEIETVIRGVTSGEVPDYQVSALLMAIC
                     WKGMDVTERSYLTSAMASSGRKLDFSSVKGTKVDKHSTGGVGDKISLMLAPWLAAAGK
                     KVPMISGRGLGFTGGTLDKLGAIPGYHFESDPARLSALLKSTGCFIIGQSADIAPADK
                     KIYALRDVTATVDEISLITASILSKKLAEDLDELVMDVKCGSGAFM"
BASE COUNT          721 a          994 c          940 g          674 t
ORIGIN      
        1 gcaatggtgc cggcaagagt atgctctttc gcgcacttct cggtcagtgc ccgctcactg
       61 tgacaggcag cgtgcaggtt cactataaac gcctcagcct gtttgaccag gaccagacca
      121 gcattgcgcc gcagaccagc gtgctctcac tcgcccgcga aaattttcct gcgcgcgacg
      181 agggcgaggt caggcgacta ctgggtgctt ttggttacgg tggttctgca gttttccgcg
      241 attttcggca gctgtcaacg ggggagcgag tacggctgct ggtgctcttg ctgagcaaat
      301 ctgacaattt ccctgacctg ttgctggctg atgaagccga aacagggtta gaccgcgaaa
      361 cgcgcgccct ctttgcccgg ttcttacacg agtacccggg cctgttgttg ttcacgacgc
      421 acagcgaaga attcgccgcc gcggttaaac cgagccgcag catcactctc acgcggccga
      481 ataatttcag cgcagagcct gcaacagcag ctcacgctgc gctgcaccct cgcgaataaa
      541 ggcgacaggt tgacccagcg gtgccgcaaa ggtgtgccat tgcggtcctg taactttttt
      601 gccggttgaa gactcgatcc aggtatcggc gggcagatag acgcggccac tcgttgccgc
      661 gggctcgatg accggtgcta ccatcaaatc gcgaccaagt agaaactggt ggcgcagcag
      721 ataggtttcc tgctcagccg gataatgcag aaacaccggg cgcgcgagcg gcataccaga
      781 tttggccgcc tcggccatca gttgcgcaaa atatggcttc agcgccaggt gctgccggcc
      841 gagcgccgcg aattcagcga caatcttttc gtcgctgtag gcctggtaat ttttctccgg
      901 gcggttgcct tcgtgtgtac ggaagaatgg cgtaaacacc gcgaactccg cccagcgcaa
      961 aaagagctca cggctgcggt ggtaattttt gacagggtta ttaatcgtgg tataaccgcc
     1021 gatgtcgctg tggttgagag taattcccga aatacccccg gtcaaaagcc cggtcactgc
     1081 agaagcgagg ccgtcgtatt cgtcaaagct caccatctgg tcgcccagcc agaagagcgt
     1141 tgagtgctcc gccgaaccgg caaaacccgc gcgggtaaag aataccacct cacgctcgag
     1201 gcctgcctcg cgtatggcct cgcggttgag ccgcgcccat tcgaccgggt accggttgtg
     1261 ccaatgcgcg gcgctttcac ccgatgccag ctgagcatcc cacggcagcc actcgccaaa
     1321 atcggccata taacccgcaa ggccattgcc aataagatta tttctgataa tattcttcag
     1381 gaaggtacgg gcttcggcat tcgagaggtc gaccaggtat gccggaaaac cagcggtctg
     1441 tatcttgtac ggttcaccgt ctctcttgcg cacgaggtac cccttcgctt tcgcctcggc
     1501 aaatattggg ccctcatcgg caaggaacgg gttgatatag cccaaaacgc gcactccccg
     1561 tgcgcgcagc tgctgcaccc aggcgcgaaa atttgggtag gcagtttcat cgggcagcca
     1621 gcgccaccac agctgcgagc caaagcccgt caggcggcga cccacccagt cctgtatcca
     1681 gattgccgtt acggggtttc ctgctttgag cgcagcgttc agaacctgtt cgaccttgtc
     1741 ttttccgcct tgtacaccga gtatggtacc cgatgcccat tcgggcaagg gctgcattct
     1801 gcccgaacgc gccgtgtaac gcttcatcag ttcgccgtaa gcaaacgcgg tactgaagcg
     1861 aagctcgaga tagttgtccc atatttcgac acagtagtgg tcactcttga aatcccacag
     1921 ctggtaggct gtctgcgacg tttcaaaaga acggaaagca gaggtcatga agaacggcac
     1981 gggagcatag gtggtaacgt cgcggccgcc agcaccagct gtgaggtttg ccccgatggt
     2041 aatcggctgc gcaccgcggc caataccctg ttcttcgctc agcattggta tgcgccggcc
     2101 gcgcaggtcg aggtgcgtga actgctcgcc gccgccaaat acacgctcgc cctcttctgc
     2161 catgaagctg aacagcagcc ggttgaaccg ggcatcgcgt ggcctgatct tcagcgcgac
     2221 atggtctgcg gcctgcgtaa aagagatttc gatggctgcg ggagtgccgg aacaactgag
     2281 ctccgccgcc agattgattg ctgcagtgga cgcttcaccc gccgagatcc tttgcagctc
     2341 gcagtgttcg cgcaccactt cgtgcatctt gtagcttgcg aggcgttccc tgacggcatc
     2401 ggtcgccacc ctcgcagaga gaaaccccgg tgccagaggc aatctcagga caacccggtt
     2461 tagcctgaca tccatcaggc gcagatactg acctgcaggt tggtattcga gcaacaggtc
     2521 gccggctttc agcccacctg acagttcggt tggtaccgtt acctgctgcc ggccacagtg
     2581 caccaaaaaa agcaatccca agacgaaaat accgcgatgc atcagcgaga cttgcgccgc
     2641 cgcctgcgcg gtcaatcaat gaccggcccc ttattcactt gcccgcggcg ttggcatttg
     2701 cagaatttac catgaacgac atcatcgcgc gtaaaaaagc aggccaaaag ctcacgaaac
     2761 aggaaattga aaccgtaatt cgcggggtca ccagcggcga ggtgcccgat taccaggtca
     2821 gcgcgctttt aatggcaatc tgctggaaag gaatggatgt caccgaacgc agctacctga
     2881 caagcgccat ggcctcgagc ggaaggaaac tggatttttc ttcggtaaaa ggcacgaagg
     2941 tcgacaaaca ctccaccggt ggcgtgggag acaaaatttc gctgatgctt gccccctggc
     3001 tggcagcagc cggcaagaag gtgcccatga tctcgggccg aggcctcggc tttaccggcg
     3061 gcacactcga caaactcggc gcgattcccg gttaccactt cgaaagtgac ccggcgcggc
     3121 tgtctgctct gctcaaaagc accggttgtt tcatcattgg gcagagcgcc gacatagcgc
     3181 ctgccgataa aaaaatctac gcactgcgcg acgtcactgc tacggtcgac gaaatctcgc
     3241 tcatcaccgc ctcgatactc tccaagaagc tggcagaaga cctggacgag cttgtgatgg
     3301 atgtcaagtg cggcagcggg gcatttatg
//
LOCUS       DAOQJF010000363         3326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18624_length_3326_cov_3.296240, whole genome shotgun sequence.
ACCESSION   DAOQJF010000363 DAOQJF010000000
VERSION     DAOQJF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18624_length_3326_cov_3.296240"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..780
                     /locus_tag="PLF85_14995"
     CDS             202..780
                     /locus_tag="PLF85_14995"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE21026.1"
                     /translation="MKRENKTQYALLGILTQYECTGYDMKKFIETSIGYFWSESFGQI
                     YPNLKKLEQQGLIRKARTADAKGSDRIIYAITPAGRKKLQQWLAKPAEPLAERNELLL
                     KLFFGSQLDHDGIRQHLRRHQQTGEETLKVYAGVEKDIAVYKDKSPDYPFWRMTLDYG
                     VERTKMELKWVKSANEILDSIAKTKKTGRKKS"
     gene            777..1358
                     /locus_tag="PLF85_15000"
     CDS             777..1358
                     /locus_tag="PLF85_15000"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNE21027.1"
                     /translation="MKRILVIQGHPRSESLCGSIAREYAAAAARGGAQVDLLDLPKLQ
                     FDPILREGYKAEQALEPDLVKAQQLIYGSDHLVFVFPSWWASMPALLKGFIDRVFLPG
                     FAFKYRKNSPLPEKLLKGKTARIFITMDAPGWYYRWFNRAPGLRLLKFATLEFCGVKP
                     VKYHIFGPVRGAPEKRLLKFLNEARRTGAQEAK"
     gene            1355..1903
                     /locus_tag="PLF85_15005"
     CDS             1355..1903
                     /locus_tag="PLF85_15005"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNE21028.1"
                     /translation="MKKFKMAFAVELAIIGALTTLLFVGVTLPEVFPRAVHRFLHILG
                     AIIFLGNIVVSALWFSVAVASRKNDWFAFAARLMNITDLVFTGPGLILLLWNATVLVG
                     AYGNPLAVDWLKWALIYFAATGALWMGVLIPLQFRFMKQTDAGNMPWNDAREQKLLGL
                     YNAAGGAAVLLIILSLVKMVLK"
     gene            1990..2799
                     /locus_tag="PLF85_15010"
     CDS             1990..2799
                     /locus_tag="PLF85_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21029.1"
                     /translation="MTSYRNFSLKLRYAMIPVILVLTMYPSEPPAPCSGQNNPYLLQN
                     LQSAEFTLPRYNSEDVWGKTGAKEGAWELWATSTLTAEGKPPKIIADGPDALESQGKN
                     YTWSCMFSVGSLIDKKTDTAWSEAVEGDGIGQAVLFPVKYFPRGKRKTATLKIFNGYG
                     KSQRIWKNNNRVKDLVLHIASAQAGGIETWTSYSQIKSHCKVPLQLKDQFGYQKITVP
                     LEGCNWNVQISKEDREMGIEIKEALVIGIEIVSVYPGDKYKDTLISEADFY"
     gene            complement(2813..3160)
                     /locus_tag="PLF85_15015"
     CDS             complement(2813..3160)
                     /locus_tag="PLF85_15015"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE21030.1"
                     /translation="MNTVVYPAFKDPLGGLSLDEFRSQLREKLTHRVQSVWLYGSVAR
                     GEARPDSDVDLFIVTDTDLPFHERGSLFDDLRDFNANIEPLVYTPAEWLRLTSEPTVG
                     FWQSAVREMVKLL"
BASE COUNT          784 a          837 c          928 g          777 t
ORIGIN      
        1 ctctccccag ccataggctt aagcccatgg ctggggagaa ccgttgctgc ggtcgttgtc
       61 aatattgggc aaacttcttg gttcaatggc cagagatgcc ggcgcaaatc tttcagctac
      121 gggcttaagc ccgtagctga aagagagaaa atgtgtgatt tttcttgccg gtatatctaa
      181 tagatagtat ctattagttg aatgaaacgc gagaacaaga cgcagtacgc cctgctcggt
      241 attttgacgc agtacgagtg tactggttac gacatgaaga agttcatcga aaccagtatc
      301 ggctatttct ggagcgaaag tttcggccaa atctacccca acctgaagaa gctcgagcag
      361 caggggttaa tccgcaaagc gcgcaccgcc gacgcgaagg gttctgatcg catcatttac
      421 gccatcaccc cggccgggcg caagaagttg cagcagtggc tggcaaaacc tgccgaaccg
      481 ctcgccgaac gcaacgagct tttgctcaag ctattctttg gcagccagct cgaccacgac
      541 ggcattcgcc agcacctcag gcgccaccag caaacaggcg aagagactct caaggtttat
      601 gccggcgtcg aaaaggacat cgctgtgtat aaagacaaat cgcccgacta cccgttctgg
      661 cgcatgaccc tcgattacgg tgtcgagcgc accaagatgg agctcaagtg ggtgaagtcg
      721 gccaacgaaa ttctggattc gatcgctaag actaaaaaga ccgggagaaa aaaatcatga
      781 aaagaattct tgtgatccag gggcatccac gctccgaatc gctgtgcggc agcatcgcgc
      841 gcgagtatgc agcggcagct gcgcgagggg gagctcaggt tgatttgctc gatctaccga
      901 agctgcagtt tgacccgatt ctgcgtgagg gttataaagc agagcaggcg cttgagcccg
      961 acctagtaaa ggcgcagcaa ctgatttatg ggtccgacca tctcgtgttc gtcttcccga
     1021 gctggtgggc ttcgatgcct gccctgctga aaggtttcat cgaccgagtt tttctgccgg
     1081 ggtttgcttt caagtaccgc aagaactccc ccttgcctga aaagctgctc aagggcaaga
     1141 ccgcgcgcat cttcatcacc atggatgccc ccggctggta ctaccgctgg tttaaccgcg
     1201 cgcccgggtt gcggctgttg aagtttgcga cgctcgaatt ctgcggcgtg aagccggtga
     1261 aataccatat cttcgggccg gtgcgtggtg cacctgagaa gcgcctgcta aaatttctga
     1321 atgaagcgcg ccgtactggt gcgcaggagg caaaatgaaa aagtttaaaa tggcattcgc
     1381 ggttgagctc gcgatcatcg gcgcgctcac gaccttgctt tttgtgggtg tgacgctgcc
     1441 agaggtcttt ccgcgggcgg tgcaccggtt tctgcacatc ctgggagcga tcatctttct
     1501 cgggaatatt gtcgtgagtg cgctctggtt ttctgtcgca gtggcgtcgc gcaagaacga
     1561 ttggttcgct tttgctgcaa gactcatgaa tataaccgac ctcgtcttta cggggcctgg
     1621 tctgattctg ctgctctgga acgccaccgt actcgtgggt gcttacggta atccgctcgc
     1681 ggttgactgg ctgaagtggg ccctcatcta tttcgccgct acaggcgctc tctggatggg
     1741 cgtgctgata cccttgcaat ttcgcttcat gaagcagaca gatgccggaa atatgccgtg
     1801 gaatgatgcg cgggagcaga aattgctcgg tctctacaat gcggcgggcg gagctgcagt
     1861 gttgctgatc attctgagtc tggtgaagat ggtgttgaag tagattcggc ccgtcgtgat
     1921 ggcttactct tgagcgaaag actctcaaat aaatactcgt cgttttgatg tgggttaata
     1981 gtgcaggtca tgactagtta ccgaaacttt tctctcaaac tgcgctacgc aatgatacca
     2041 gtgatactgg ttctgaccat gtatccatcg gagccgcccg ccccgtgttc agggcagaat
     2101 aacccttact tgctgcaaaa tctacagagt gccgagttta ccttgcctcg ttacaatagc
     2161 gaagacgtct ggggtaagac cggagccaaa gaaggtgcct gggaactttg ggcaacttcg
     2221 acgctcactg ccgaaggcaa accgccgaaa attatcgccg atggccccga tgcccttgag
     2281 tcgcagggaa aaaactacac ctggagctgt atgttttcgg tcggctcttt gatcgataag
     2341 aagaccgata ccgcgtggtc agaagccgtt gagggcgatg gcatcgggca ggcagtactt
     2401 tttcccgtaa aatattttcc gcgcggcaag cgtaagacgg cgacgctgaa gatctttaac
     2461 ggctatggca aatcgcagcg catttggaag aacaataatc gcgtgaaaga cctggtgctg
     2521 catattgcga gtgcgcaggc ggggggcatt gaaacctgga cgtcctattc gcagattaaa
     2581 tcgcactgca aagttccgtt gcaactaaag gaccagtttg gttatcagaa gattaccgtt
     2641 ccgctcgaag gttgcaattg gaatgtccag atatcgaaag aagacagaga aatgggcatt
     2701 gagatcaaag aggctctggt catcggtatt gaaatcgttt cggtttatcc tggcgataag
     2761 tacaaagata cgctgatttc tgaggcggat ttctactgag cgcgcggagt aatcaaagca
     2821 gcttcaccat ctcacgcacc gcactctgcc aaaaaccaac agtaggctct gaggtcaggc
     2881 gcagccattc tgcgggcgtg tataccagtg gttcgatgtt tgcattaaag tcccgcaggt
     2941 cgtcgaagag agagccgcgt tcgtgaaatg gcagatcggt atcggtaacg ataaacaaat
     3001 ctacatcact atcgggccgc gcttcgcccc tcgcgaccga gccatatagc cagacgcttt
     3061 gcacacggtg ggtgagcttt tcccttagtt gcgacctgaa ttcgtcgagg gaaaggccgc
     3121 ccagcgggtc tttgaatgcg ggatagacga cagtattcat gcggactctt gtatcagctt
     3181 cttcacccta ttgagaatta gtaacgcttc ggcttgcgca gaaattgctg cttcacgatc
     3241 cagaaaatca ctgggtattc cctcgggaaa agcgtcggga tagcgtccgg agatatagta
     3301 acggtcgaga atcttgcccg cgcgtt
//
LOCUS       DAOQJF010000364         3318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18694_length_3318_cov_3.448667, whole genome shotgun sequence.
ACCESSION   DAOQJF010000364 DAOQJF010000000
VERSION     DAOQJF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18694_length_3318_cov_3.448667"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLF85_15020"
     CDS             complement(<1..986)
                     /locus_tag="PLF85_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNE21031.1"
                     /translation="MIKTPSTNYNEVVPPGEDEKFAAYAQKLRAIQKAKSKKYGKGRL
                     LHRKGLLALRAELRVDANLPAHAAQGIFSKAGKYEAVIRLSNGGLDVKADKVPDIRGL
                     AIKVKGVAGKSALTGKPHTEQDFLLINHTTFGSARAEEFLDLLLALNAGGGAVLKHLY
                     RTHGLLGMFRAMGRLAKIIGKPFKGYAAENLSTVAPVKNGAYAIKLRIRPLTKIAVPL
                     DKSDLAHDLRTHLKSQPIDYAVEAQFFTDAESTPIEDATVEWSEEVSPFVQVGTLHVP
                     AQHLAGEEYESFAAAVEQMKFDPWNAIEEHRPLGNIMRARKHAYYVSQNERGV"
     gene            complement(1026..2138)
                     /locus_tag="PLF85_15025"
     CDS             complement(1026..2138)
                     /locus_tag="PLF85_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNE21032.1"
                     /translation="MEITANITTLGNMVMRNPKVAAELFPSAIAGYVLQQGHNNTLDV
                     VSGHAVHYDWTYSNFGNPEFKSLYRRAYKGQWDAEDLPWDTNVDPFNPNIAIIPERLV
                     PGFGEKFYPKDLRDRQKIQHAAISFLLSQFLHGEQGALYIAGETVEASPWFDAKLYGS
                     TQVVDEGRHVEVFARYLQQKLEKLYHVNDNLFVILRSITAGSDWDLKFLGMQIMIEGL
                     ALGAFGTLYKFTEEPLLKQLLKQVISDEARHVHYGVVALRDYFTKEISEAKRREREDW
                     AYEVAIMLRNRFHFLEIHEEFYGQSISRKDWLHLLDTSEMMSEFRKQLFTRMIPNLKA
                     IGLLSDRIRPKYAQLGILKYENERSADHLTAEDLMR"
     gene            2466..2693
                     /locus_tag="PLF85_15030"
     CDS             2466..2693
                     /locus_tag="PLF85_15030"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNE21033.1"
                     /translation="MKTTLDIPEEKFTTVQDLYGLRTKREAVIFALDELTRRYKIERL
                     VDQLGTFTDFMTQDDLRHMRDLDTARDISLN"
     gene            2662..3045
                     /locus_tag="PLF85_15035"
     CDS             2662..3045
                     /locus_tag="PLF85_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE21034.1"
                     /translation="MILLETSALIEAWRKNGRPDAKARVESALRSGRAALCQPVVLEL
                     SAGMKKSEEFKLLHDYLDVLPLLPITDAVWQLAASRARLFRANGLTVSNFDTLIFATA
                     EHHNANIVTIDGHFAQMREVLKTCT"
BASE COUNT          734 a          858 c          866 g          860 t
ORIGIN      
        1 acacctctct cattttgtga tacataatat gcgtgcttac gggcgcgcat gatattgccg
       61 agcggacggt gctcctcaat agcattccac gggtcaaact tcatctgctc tacagcggcc
      121 gcgaatgatt cgtattcttc gccggcaaga tgctgtgctg gcacgtgcag cgtacccacc
      181 tggacgaagg gacttacttc ttcgctccac tcgaccgtcg catcttcgat cggggtgctc
      241 tcagcatcgg taaagaactg cgcttcgacc gcgtaatcga tcggctgcga tttcaggtgt
      301 gtacgcaggt catgcgccag gtcactcttg tcgagcggca ctgcaatttt ggtcagtggc
      361 ctgatacgca gcttgatggc gtaggcgcca tttttgactg gtgccactgt gctcaggttc
      421 tcggcagcat aacctttgaa gggtttaccg ataatttttg ccaaccgccc catagcccta
      481 aacattccca ataagccgtg cgtgcggtag agatgcttca gtaccgcgcc accgccggca
      541 tttagcgcaa gcagcagatc caggaattct tctgcgcggg cagagccgaa agtcgtatgg
      601 ttgatcagca ggaaatcctg ctctgtatgc ggtttgcccg tcagcgctga ctttccggcg
      661 acacccttaa ccttgattgc cagcccccgg atatcgggaa ccttgtccgc ttttacgtcg
      721 agccctccgt tagagaggcg aatgactgct tcgtatttcc cggctttgga aaagataccc
      781 tgcgctgcat gcgcgggcaa attcgcgtcc acccggagct ccgccctgag cgcaagcagg
      841 cctttacggt gcagcagcct gccctttccg tattttttcg atttggcttt ttgtatggcg
      901 cgcaattttt gcgcgtaggc ggcaaatttt tcgtcttcgc cgggtggaac aacttcgttg
      961 taattcgtgg atggtgtttt gatcatttac agcagtggct gcaaagcagc cactgctgta
     1021 aatgcttatc gcatgagatc ttcagccgtc aggtgatcgg ctgaacgctc gttttcgtac
     1081 ttcaggatac ccagttgggc atacttcgga cggatacggt cgctcaggag gccgattgcc
     1141 ttcaggttcg gaatcatgcg cgtgaacagc tgcttgcgga actcgctcat catttctgac
     1201 gtatcgagca ggtgcagcca gtccttgcgg ctgattgact ggccgtagaa ctcctcgtgg
     1261 atttcgagga agtggaagcg gttgcgcagc atgatagcca cttcatacgc ccagtcttcg
     1321 cgctcgcggc gtttcgcctc tgaaatttct ttagtaaagt aatcgcgcag cgcgacaact
     1381 ccgtagtgga cgtggcgtgc ttcgtcagag atcacctgct tcagcagttg cttgagcagc
     1441 ggctcttcgg tgaacttgta gagagtaccg aatgcaccga gcgccaggcc ttcaatcatg
     1501 atctgcatgc cgagaaattt gagatcccag tcagaaccag cagtgatgga acgcagaatg
     1561 acaaagaggt tgtcgttcac gtggtagagt ttttcgagtt tctgctgcag gtagcgcgca
     1621 aaaacctcta cgtggcgacc ttcgtcgacg acctgcgttg agccgtagag tttggcgtca
     1681 aaccaggggc ttgcttcgac ggtttcaccc gcgatgtaga gcgcgccctg ctcgccgtgc
     1741 agaaactgcg agagtagaaa gctgatcgca gcgtgctgga tcttctgccg gtcgcgcagg
     1801 tctttcggat aaaatttttc gccgaaacct ggtaccaggc gctcggggat aatcgcgata
     1861 ttggggttga aggggtcaac attggtatcc cacggcaggt cttctgcgtc ccactgtcct
     1921 ttataggcac ggcggtagag cgacttgaac tcggggttgc caaagttaga gtaagtccaa
     1981 tcgtagtgaa ccgcatggcc agagactacg tccagggtgt tgttgtgccc ctgttgcagt
     2041 acgtaacctg cgattgccga cgggaacagt tctgctgcaa ccttggggtt gcgcatgacc
     2101 atgttgccga gggtcgtgat gtttgctgta atttccatag tttcctcctc tatgtcgtct
     2161 agccttagct ttcaccaaaa ctattcagca cttgatagtt ttgtcgacaa ttcggagcgt
     2221 cgcgtcaatc gtttttttgt tcgcagtgca caaaagttcg ccactttttg gcactaaagg
     2281 tatattcgct atgcaaattg aggatatttt catcttttta tccttattgt ttctgcgatt
     2341 ctttgcgcca cgcaggctag tgccacgtgt gccaattgag catagagccg gaagccccct
     2401 ggaagccgga acagcagaca tacaatctta attgactata tatataatta tattatttta
     2461 tacatatgaa aacgactctt gatattcctg aagagaagtt tacgaccgtg caggatttgt
     2521 atggcctgcg gacaaagcgc gaagccgtaa tctttgcgct cgatgagctg acgcgccggt
     2581 acaagatcga acgactggtc gatcagctgg gcaccttcac cgacttcatg acacaagatg
     2641 acctgcgtca catgagagat cttgatactg ctcgagacat cagccttaat tgaagcctgg
     2701 cgcaagaacg gccgtcctga cgcaaaagcg cgggtcgagt cagcgctgcg ctcgggccgg
     2761 gcggcactct gccagccagt cgtcttagaa ttgagtgcag gcatgaaaaa aagtgaggag
     2821 ttcaaactac tgcatgacta tctcgatgtg ctgccactct tacccatcac agacgctgtc
     2881 tggcaactcg cggcaagccg cgcacggctg tttcgcgcca atggtctcac agtctctaat
     2941 ttcgacacgc tcatcttcgc cacggccgaa caccacaacg ccaacattgt cacgatcgat
     3001 ggccatttcg cacagatgcg cgaggtgctg aagacttgca cttagtaggg gtaggtttaa
     3061 acctacccct actaagtgca taattacata tatatcgaac ttttattctt tacatttcct
     3121 gtataatctg cctattggca ctgacatact atccgcgagg atatattgtt gcagcatcgg
     3181 ccttcgggcc gaaaaaagaa aatttctcaa ggagataaca tgaaaaaaat cattatcgcg
     3241 tcagcactgc tgctggcagc aacgacttcg agctttggtg cagttaaagc cggaaaattt
     3301 ggtatggatt ttagtttt
//
LOCUS       DAOQJF010000365         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_18833_length_3305_cov_4.408615, whole genome shotgun sequence.
ACCESSION   DAOQJF010000365 DAOQJF010000000
VERSION     DAOQJF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18833_length_3305_cov_4.408615"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PLF85_15040"
     CDS             <1..1160
                     /locus_tag="PLF85_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE21035.1"
                     /translation="TGKPLKNLALNEVVAVVYTEEALGVTDLAQTWVRVRAADGREGY
                     VRLGNLSKEKQPEPEFAVAFRDIEKKAYVTADALYVRSAPNRSATEQGMLIRNAEVNV
                     LEYSDRDDYIDGMTAKWARVNSGGETEGWVFSGYLSDEPRAPSLGPNNEAKEDPNHIQ
                     SGSSKTVRPPLLAVRDEPTRYGTVIGRIRQGKSVRILERLTAWENLAGLRSVWVRVRY
                     DDLEGWVYGGFLSSSGYTMSSDSLDKPFILPIDPSQYRRTSKYGPRTHPINKSPSFHS
                     GVDLAAAGGTPIYAAGDGVIEVQNDNTGYGMLTVIRHENGLVSYYAHQSRRHKSVGTR
                     VTAGELIGEVGTTGNSTGNHLHFEVRTNYNDSHFNPDLYVPFPEAQEVEGP"
     gene            1214..>3305
                     /locus_tag="PLF85_15045"
     CDS             1214..>3305
                     /locus_tag="PLF85_15045"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE21036.1"
                     /translation="MLRQKKYCGIAAILLLTSPVFAGPQEDFLAAAKRSNLKLAQKAH
                     AAGGVEVNVIDDKGKNAVMYACENGSAPFLTWLAEVQADLSVTDAEGNNCYHLALKAR
                     NPAPVFTLLAEKSINKNAQNQKRETPLMAAVSQGKKGALDLLIKLQPDLNASDAEGYS
                     PLARSIQRRRYDISNALLLAGANANGEPYAPLVLAYDAGNLKLFDALAKAGANLNVVH
                     AKSGQALLLEAIKKERLAYARLLIEAGANTSITDSNGETLPVICAKQTIPDLIPVLVQ
                     KGHSIETKDAYGKTLLQIAHENILKRLSAGREKLFAVLLESGANPNTLSPSGRSVLME
                     QSESGRYNQVKVLLERGADVNFRDKAANTVLHTTAQRNQLGTMRLLVEKFSDINVTGD
                     SGNTATHFAARAGGTGILKLLKDRGANLELKNNAGDTALSIAIGRQDPTTTRALIALG
                     ASLSDEGRETPLMLEIAKSGVVNSRTVELLTVLNKAGANINAVNRFGNNALAYALNRK
                     NMKMADALLKAGAKSEAGDARGNTLLHKLALSAKFNKLKNQELTDWLHLVLSYQHPDY
                     QTPAGETALHIAANKDNNPDAEAAQQLFETLVNYSASVVVRDARGRTPADLARQLDLQ
                     ALANASIPVAATQNIAQALQTPENDKLLQVVAADREFFALHQQGKVLRLLSFSENLET
                     KAVREVPARDVAGQR"
BASE COUNT          777 a          931 c          971 g          626 t
ORIGIN      
        1 aaacgggtaa gccgctcaag aatttggcgc tcaacgaggt ggtggcggtg gtctataccg
       61 aagaagcgct cggcgttact gacctcgcgc agacgtgggt gcgcgtgcgc gcagccgacg
      121 gtcgcgaagg ctatgtgcgg ctcggtaacc tgagcaaaga aaaacagccc gagcccgaat
      181 ttgccgtggc gtttcgcgac attgaaaaga aggcatacgt caccgccgat gcgctctatg
      241 ttcgctctgc gccaaaccgc agcgccaccg aacagggcat gctgatccgt aatgcagaag
      301 tcaacgtact cgagtactct gaccgtgacg actacattga tggcatgacc gccaagtggg
      361 cccgggtgaa ttcggggggc gagacagagg ggtgggtttt ctctggttac ctcagcgacg
      421 aaccgcgcgc accttcgctg ggccccaata acgaagctaa agaagatccg aaccacatcc
      481 agtcaggttc gtcgaagaca gtcagacctc cgctgctggc ggtgcgcgat gagcccacgc
      541 gctatggtac ggtgattggt cgcatacgcc agggtaaaag cgtgcgtatt ctggagcgcc
      601 tcaccgcgtg ggagaacctg gcgggtctgc gctcagtttg ggtgcgcgtg cgctacgacg
      661 atctcgaagg ctgggtatac ggcggcttcc tctcttcttc cggttatacg atgagttctg
      721 atagtctcga taagccgttc atactgccga ttgacccttc gcagtaccgg cgcaccagca
      781 agtacgggcc acgcacgcac ccgattaaca agtcgccgtc gttccattca ggggtcgacc
      841 tggctgctgc aggtggtact cccatctacg ctgcgggcga cggagtgatt gaagtgcaga
      901 acgacaatac tggttacggc atgctcacgg tcattcggca cgagaatggc ctcgtatctt
      961 attatgcgca ccagagccgc cgtcataaga gtgttggtac ccgcgtgaca gcgggcgaac
     1021 tgatcggcga agtgggcacc accggtaatt ccaccggcaa ccacctgcac tttgaagtga
     1081 gaacaaacta caacgactcg cattttaatc ccgacctcta cgtgccgttt cccgaagcac
     1141 aggaggtgga aggcccatga gcgcgcatgg aggcgctccc gtgcgaatgg gcagaagcac
     1201 aggaggtgga ataatgttgc gtcaaaagaa gtattgcggc attgccgcaa tacttctttt
     1261 gacttctcct gtttttgccg ggccgcagga agattttctc gcggcggcaa agcgcagtaa
     1321 cctgaaactt gcgcagaagg cccatgcggc cggcggggtt gaggtaaacg tcatcgacga
     1381 caaaggtaag aacgcggtca tgtatgcctg tgaaaatggc agcgctccct ttctcacctg
     1441 gctcgccgaa gtgcaggcag acctgtcggt tactgatgcc gagggcaaca actgctacca
     1501 cctggcgctc aaagcgcgaa atcccgcacc cgtttttacc ctgctggcag aaaaaagtat
     1561 caacaagaat gcgcagaacc agaaacgcga aactcccctc atggcagcgg ttagccaggg
     1621 taagaaggga gcactcgatt tgctgatcaa actgcagcct gatctcaatg cctctgatgc
     1681 agaaggttat tcgccgcttg cgcggtcgat ccagcggcgg cgttacgata tcagtaacgc
     1741 gttgctcttg gccggcgcca atgcgaacgg tgagccgtat gcgccgctcg tgctggcata
     1801 cgatgcaggt aacctgaaat tgttcgatgc cctggcgaag gccggggcca acctgaatgt
     1861 cgtacacgcc aaatcggggc aggctctgct gctcgaggcc atcaagaagg agcggctggc
     1921 gtatgcgcgc ctgctgatcg aagcaggagc caatacttct atcaccgact ctaatggtga
     1981 aaccttgccg gtgatctgcg cgaagcagac gatacccgac ctgattcccg tattggtaca
     2041 gaaagggcat tccattgaga ccaaagatgc ttatggcaag acgctgctgc agattgcgca
     2101 cgaaaacatt ctgaagcgcc tgagtgccgg ccgcgagaaa ctttttgccg tgctgctcga
     2161 atcgggggcc aacccgaata cactttcgcc gagcggccgc agcgtgttga tggaacagtc
     2221 cgaaagcggg cgctataacc aggttaaggt gctgctcgaa cgcggcgcag atgtcaattt
     2281 ccgcgataag gctgccaaca ctgtcctgca cacaacggcg caacgcaacc agctcggtac
     2341 catgcgcctg ctggtcgaaa aattctccga tatcaatgtt accggcgaca gtggcaatac
     2401 ggcgacgcac tttgctgcac gcgcgggtgg cacaggaatt ctcaagctac tcaaagatcg
     2461 gggggcaaac cttgaactga agaacaatgc cggcgatacg gcgctctcga ttgcgatcgg
     2521 ccggcaagac ccgacgacaa cacgtgcgct cattgcgctc ggcgcatcgc ttagcgacga
     2581 gggcagggaa actcccctca tgctcgaaat tgccaagtcg ggcgtcgtca acagccgtac
     2641 cgtggagcta ctgacagtac tcaacaaagc gggcgccaat atcaacgcag tgaatcgttt
     2701 tggcaataat gcgctggctt acgcgctgaa ccgcaagaac atgaaaatgg ccgatgctct
     2761 gctgaaggcg ggggccaaaa gcgaggcagg cgatgcgcgg ggtaataccc tgctgcacaa
     2821 gctggcgctg agcgcgaagt tcaataagct caagaaccag gaactcaccg actggctgca
     2881 cctggtgctg tcgtaccagc atcctgacta ccagacaccc gccggagaaa ctgcgctgca
     2941 tatcgctgca aacaaagaca ataaccccga tgcagaagct gcgcagcagc tgtttgaaac
     3001 gctggtaaat tattcggcga gcgtggtcgt gcgcgatgca cgtggtcgta cacctgccga
     3061 cctcgcgcgc cagctcgacc tgcaggcact ggccaatgcc agcattcccg tggcagcaac
     3121 gcagaatatt gcgcaggcat tgcagacgcc agaaaacgac aagttgctcc aggtggttgc
     3181 tgcagaccgc gaatttttcg ccctgcacca gcaagggaaa gtcctgcggt tactgagttt
     3241 ctcagaaaat cttgagacaa aggctgtgcg cgaggtacca gcgcgcgatg tcgccgggca
     3301 gcggc
//
LOCUS       DAOQJF010000366         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_19113_length_3273_cov_5.254506, whole genome shotgun sequence.
ACCESSION   DAOQJF010000366 DAOQJF010000000
VERSION     DAOQJF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19113_length_3273_cov_5.254506"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1273)
                     /locus_tag="PLF85_15050"
     CDS             complement(137..1273)
                     /locus_tag="PLF85_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNE21037.1"
                     /translation="MRNTSLPLAFVVAFVSLYSSMTCTRVAAAPAETVVVSKPQEAAA
                     APVLLANAAPAEPEKPAVEAKDVDKQPRVVRFLQKLREYQLSWAQFPVKRPDFMKGIY
                     LTNATAVNTKSLAHFLRQSKKYGINTLVIDAQGQPISADQMSMIKSAGIYPVARIVCF
                     DLGLKQKYPDQKHVDKIMASVERAAKSGFREIQLDYIRYADEKHLQRLPLKFKYDQIA
                     TVLSRARKLADSLGVELGADVFGRITLNKNDHIGQKLELFGQYVHNLYPMVYPSHYYG
                     DPEKIANPYGTVKEGVASSKERIPQTRIIPYIQGFGMKIKQSGLALPQYVLKQFFAVD
                     DAQGHGYVVWNARNDYSATWKALQEYDRLAPKRKQQKVAQAQGK"
     gene            1461..2192
                     /gene="ubiE"
                     /locus_tag="PLF85_15055"
     CDS             1461..2192
                     /gene="ubiE"
                     /locus_tag="PLF85_15055"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNE21038.1"
                     /translation="MPPREKTTALSGSADERREVVRGMFNRIAPTYDILNRILSLGID
                     RRWRSKTIERMGISERSVVLDLACGTGDLMAEAARKNAMALFGIDPSKEMLLRARSKL
                     RYALTRTYYLESFGEELPLKSEICTHAMIAFGIRNVQERSRAFAELNRVLKPGGVLAV
                     LEFTPMDRKLVSWLFNLYFQKILPLIGGLISRDRQAYQYLPESVARFVTSEDLIREAE
                     GVGFTHRESKQFFFGVATAILFEKP"
     gene            2341..>3273
                     /locus_tag="PLF85_15060"
     CDS             2341..>3273
                     /locus_tag="PLF85_15060"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNE21039.1"
                     /translation="MQQKLMKSLRLTVVPVMLVSAAGLLEAQEKPAAEQKKIIAPAKA
                     DTGTEAAVKTEASAPKSPEAGKGTQTASPAASVAEKSEKSDKPEKEKADKGKLPVAIS
                     YGDKGLEFDAGEKFSLAMQHRLQFRYAYPFDADPRQLSDMDAEGHSFMVRRARFRMGG
                     HAFTKWLKYGFQYDWSQPVLRDFYIDVARFSWATLRVGRAKVIYNDERVASSGRQQMV
                     NRSILNDVFTVDRQQGLQLMGRLFDNTPADLNYVVGVFSGRGVGERFNDDVNMMYAAR
                     LQWNAVGDPIEWSQSDHKYTQRWQLNIAAGAATNK"
BASE COUNT          702 a          882 c          944 g          745 t
ORIGIN      
        1 atcttcgcag gcgatgaagt cggcattctc gaggacgtag cgcgcgcgtt gcgaaaggtc
       61 gtcggcgttg ccgatcggca gcgctaccga atacagtgcc ccccggacca gcggcgcgcg
      121 ctcaccgggg ttcatattac ttgccctgcg cctgggctac cttttgttgc ttgcgcttgg
      181 gagcgaggcg gtcgtattcc tgcagcgcct tccaggttgc cgagtaatcg ttgcgtgcgt
      241 tccagacaac gtaaccgtgt ccctgcgcat cgtcgacagc aaagaactgc ttgagtacat
      301 actgtggcag cgccaggccc gattgtttga tcttcatgcc aaaaccctgg atgtagggaa
      361 taatgcgcgt ctgcggaata cgctccttcg agctcgccac accttcttta accgtaccat
      421 aaggattcgc gattttttcc gggtcgccgt agtagtgcga tggataaacc atcgggtaga
      481 ggttgtggac atattggcca aagagttcga gtttttgacc gatgtggtca ttcttgttca
      541 gggtaatacg gccaaagaca tcggcgccca gctctacgcc gagtgagtcg gcaagtttgc
      601 gcgcgcgtga aagcacggtg gcaatctggt cgtatttgaa tttcagcggc aggcgttgca
      661 ggtgtttttc gtccgcgtaa cggatgtagt cgagctgtat ttccctgaac cccgattttg
      721 ccgcgcgctc aaccgacgcc atgatcttat cgacatgctt ctggtcggga tatttttgct
      781 tgaggcccag gtcaaagcag acaatccgcg ccaccggata aatgcctgct gatttgatca
      841 tcgacatctg gtctgccgag atgggctggc cctgcgcgtc gatgaccagc gtattgatgc
      901 cgtacttctt gctctggcgc aaaaagtgcg caagcgattt ggtattcacc gctgttgcat
      961 tggtgagata aatacctttc atgaaatcag gacgtttgac cgggaactgc gcccacgaca
     1021 gctggtattc gcgcagcttc tgcagaaaac gcacaacgcg cggttgtttg tcgacgtctt
     1081 tggcttcgac cgcgggcttt tcgggctcag ccggcgcggc gtttgccagc agcactggcg
     1141 ccgctgctgc ctcttgcggc ttgctgacca caacagtctc tgccggcgcg gctgcgaccc
     1201 gtgtacaggt catgctgctg tagagtgata caaatgcgac tacgaaggca agggggagtg
     1261 aggtatttct catttctgtg ggtccgtggg gatttctcta ttttcggcaa ataacgtgcc
     1321 gatgttgatt gccggcttcg acggtttttg caagcggcac aggcggacaa accaaatggt
     1381 tggcctgtgt gcgcgggtta tcggcgtcgt gccccaaagg gcatgacgtg gcgtttcgca
     1441 ttcgtgcact gcctgcacaa gtgccaccac gtgaaaagac caccgcgctc tctggttctg
     1501 cagacgagcg ccgcgaagta gtccggggga tgttcaaccg catagccccg acctacgata
     1561 ttctcaaccg gatactatcc ctggggatag accggcgctg gcgcagtaaa acgatcgagc
     1621 gtatgggcat ttccgagcgg tcggtcgtgc tcgaccttgc ctgcggcacg ggcgacctga
     1681 tggccgaagc cgcgcgcaag aacgccatgg cgctcttcgg catcgatccc tcgaaagaga
     1741 tgctgctgag ggctcgcagc aaactgcgct acgcattgac ccgcacttat tatctcgaat
     1801 cgtttggcga ggagcttccc ctgaaaagtg agatttgcac gcatgcgatg atcgccttcg
     1861 gcatccgcaa tgtgcaggaa cgcagccggg cattcgccga actcaaccgc gtgctgaaac
     1921 ccggcggggt tctggcagta ctcgaattta cccccatgga caggaagctg gtttcgtggc
     1981 tgttcaacct ctatttccaa aagatactcc cgctgatagg cggcctgatc agccgcgacc
     2041 ggcaagccta ccagtacctg cccgaatcgg tggcacgctt cgtgacgtcg gaagacctga
     2101 tcagggaagc tgaaggcgtt ggttttaccc accgggaatc caaacagttc ttttttggtg
     2161 tggctactgc gattttgttt gaaaaaccat gaattttcct gttgagcaac gaatctatgg
     2221 ctgttttacc tgatttgtag cattaattcc ctaattaggg aatttgttaa ttagcgaaat
     2281 aatagtttac ataacatcag ttgctcccga ttagccccta ccgtatcaca cggaggaaca
     2341 atgcaacaaa agttaatgaa aagcctgcgc ctgacagtcg taccggttat gctggtctct
     2401 gcggcgggtc tgctcgaggc acaggaaaaa cctgctgcag agcaaaaaaa gattatcgcc
     2461 ccggccaaag cggataccgg aaccgaagca gcagtcaaaa ccgaagcgag cgcgccaaag
     2521 tcgccagagg cgggcaaggg tacgcaaacc gcctcgccag cagcatcagt agcggaaaag
     2581 tccgagaaga gtgacaagcc tgaaaaagaa aaggccgaca agggtaaact cccggttgca
     2641 atctcttacg gtgacaaggg actcgagttt gatgccgggg aaaaatttag cctggccatg
     2701 cagcaccgcc tgcagttccg ttatgcatac cccttcgacg cagacccgcg acagttgagt
     2761 gatatggacg cagagggcca ctcattcatg gtgcgccgtg cgcgctttcg tatgggcggt
     2821 catgctttca cgaagtggct caagtacggt ttccagtatg actggtcgca gccggtattg
     2881 cgcgattttt acatcgatgt cgcgcgcttt tcctgggcta cgctgcgtgt cggtcgcgcg
     2941 aaggttatct ataacgatga gcgcgtagcc tcgtccggcc gtcagcagat ggttaaccgc
     3001 tcgattctga acgatgtctt cacggttgac cgccagcagg gcctgcagct gatgggccgg
     3061 ctttttgaca atacaccggc cgacctcaac tacgtagtgg gtgtcttcag tggtcgcggg
     3121 gtcggcgagc gctttaatga cgacgtcaac atgatgtatg ctgcgcgcct gcagtggaac
     3181 gcagtgggcg acccgattga gtggagccag agcgatcaca agtacacgca gcgctggcag
     3241 ctcaatattg cagccggtgc cgcaaccaat aag
//
LOCUS       DAOQJF010000367         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_19146_length_3270_cov_5.344012, whole genome shotgun sequence.
ACCESSION   DAOQJF010000367 DAOQJF010000000
VERSION     DAOQJF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19146_length_3270_cov_5.344012"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLF85_15065"
     CDS             <1..488
                     /locus_tag="PLF85_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21040.1"
                     /translation="EGHLAFEVAGKTGTTSKYVDAWFVGLVSDEATVMWVGNDVNTSL
                     GAGRSGSGLCAPRYADYLRTTRYDNKPRPFTEVFEQDRIVRKSFCSKTGLLSRFPGAC
                     EGEILDQAFIRGTEPTEFDPSGAPVENGVTPQEPQAPVAPVETNPPVAPPATPAEPPQ
                     G"
     gene            492..1742
                     /locus_tag="PLF85_15070"
     CDS             492..1742
                     /locus_tag="PLF85_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21041.1"
                     /translation="MNFLIRNRILLLFTTAFIVLVVALFWPEPKKSGEMQKYYTRDAS
                     QLLSLRYRGAMSLGEKSKVLADYEILREENPVKPKEPFYRIQVHSVTTDDAALKSRVQ
                     VLAQAKTFPASALIRSIVQDWSALDFYYILPHDAAKDSEYGLQNCENSLQLKFKADER
                     AFCIGTQSQGDTRRYILDRSKNKLLITPDYTVRRVLNNIFAQREQLLQPYGADAFDLL
                     ELNIAPAMLQKLPLLSEKTGGRMRLRMLVKKDGPAPVNVWHVENLLTIKPSHAAEFAQ
                     LLIAMRIPTAFAQPALAPDAQPKDLNNALGLAEQVQPAISGSLLLKKTDKQDILLTRY
                     AFFAPGTRPGERREIQAENQLVRPMDTLAVSSFNAGYITADLFPRFAAIFQKFENDLR
                     DAVQAGKKEKSPEQAAPRKSPGKR"
     gene            complement(1748..3031)
                     /locus_tag="PLF85_15075"
     CDS             complement(1748..3031)
                     /locus_tag="PLF85_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE21042.1"
                     /translation="MQAVDESTVLVVNFLHRRLFVYSPEGKLEQDLAADSSLKGRSVF
                     VTLKPGEAAADDVVYALGRVQKDANQSWVMEQIRAVRKVNGRTVEIELAPSGRPAAVD
                     WQLARIKLTLPQCAIFRADRHQAAREFIPFSRYRVAEQTGDRILLSTTDGTPPVEFVA
                     MPDETARYFAFREGRLDAYEALGIHRRLAHDNELYEPRTMYDLVVLYAAFNVGPESAL
                     ADPALRRAINLAFDRNALCENTLLGACTGADYPVPAILSPPPQQPFPAAADYRIPVKT
                     ETAILFTLSDKERMLIAEYMRYIFKKFNIHLEFRIVDLPSMVRESNKGTRGIYLMKWS
                     GDYPHAENFLMPLFHSRNAGIGGNRAHVRDAVLDRMLDSSSYTVSETQNLQQRIRYLS
                     PWLFIGFQNMQFFRLKTSRLQIPTIFAGWGERTIR"
     gene            complement(3201..3270)
                     /locus_tag="PLF85_15080"
     tRNA            complement(3201..3270)
                     /locus_tag="PLF85_15080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3238..3240),aa:Gly,seq:gcc)
BASE COUNT          735 a          922 c          926 g          687 t
ORIGIN      
        1 ctgaagggca cctggcgttt gaggtagctg gcaagacggg tacaacttcg aagtatgtcg
       61 acgcctggtt cgtgggcctt gtctctgacg aagccacggt aatgtgggtc ggtaacgatg
      121 tgaatacttc gctgggagca ggacgctcag gcagcggctt atgcgcgccg cgttatgccg
      181 attacctgcg gacaacccgc tacgacaata agccgcggcc attcaccgaa gtttttgaac
      241 aggaccgtat cgttcgtaaa tctttctgca gcaagacagg cttgctttca cgctttccag
      301 gcgcttgtga aggtgagata ctcgaccagg catttattcg cggcaccgag ccgactgaat
      361 ttgacccatc gggtgcgccg gtcgagaatg gcgtcacccc gcaggaaccg caggctccgg
      421 ttgcgccagt tgaaactaat cctcccgttg cgccacccgc gacaccggca gagccacccc
      481 aggggtaacg catgaatttt ttgatccgta accgcattct gctgcttttc accactgcct
      541 ttatcgtgct ggtggtcgcc ttattctggc ccgagcccaa aaaatccgga gagatgcaga
      601 agtactatac ccgcgatgcg tcgcagctgc tttctctgcg ctaccgcgga gcgatgagcc
      661 tcggggaaaa aagcaaggtt ctcgcagact atgaaattct gcgcgaagaa aatcccgtta
      721 aacctaaaga gcctttctac cgtatacagg tacacagcgt cacgacggat gatgctgccc
      781 tgaaatcaag ggtccaggtt cttgcacagg caaaaacttt cccggcgagc gcgctcatac
      841 gcagcatcgt gcaggattgg tcggcgcttg atttttatta cattctgccc cacgatgcag
      901 ccaaagacag tgagtacggc ctgcagaact gcgaaaactc cctgcagttg aaattcaaag
      961 ccgacgagcg cgcgttctgt atcggtacac agagccaggg cgacacccgg cgctatattc
     1021 ttgaccgctc gaaaaataag ctactcataa ctcccgacta caccgtacgc cgtgtcctca
     1081 acaatatctt cgcgcagcgc gagcagttgc tgcaacctta tggtgcagac gcattcgacc
     1141 tgctcgagct gaatattgcg cctgcgatgc tgcagaagct gccgctgctt tcagaaaaaa
     1201 ccggggggcg catgcgcctg cgcatgctgg tcaagaagga cgggcccgca ccggtgaatg
     1261 tctggcatgt ggaaaacctg ctgaccatca aaccctcgca cgcggctgag ttcgcccagt
     1321 tactgattgc gatgcgcata ccaactgcct ttgcacagcc agcgcttgcg cccgacgcac
     1381 agccgaagga cctgaacaac gcgctggggc ttgctgagca ggtacagccg gcgatttcgg
     1441 gcagcctgct gctgaagaag actgacaagc aggacattct gctcacacgc tatgctttct
     1501 ttgcgcctgg caccaggccg ggcgagcgcc gggaaatcca ggcagagaac cagctggtgc
     1561 ggcccatgga tacgctggcg gtatcgtcat ttaatgccgg ttatattacc gcggatctgt
     1621 tcccccgctt tgctgcaatc ttccagaagt tcgaaaacga cctgcgcgac gccgtgcagg
     1681 caggtaaaaa agaaaaatcc cccgaacagg cagcgccccg caaaagcccg ggtaaacgct
     1741 agcgggctca gcggatcgtg cgttcgcccc agccggcgaa gatggttggt atctgcaggc
     1801 gcgaagtttt tagcctgaag aactgcatat tctggaagcc gataaaaagc cagggcgaca
     1861 ggtaacggat gcgctgctgc aggttctgtg tttcgctcac cgtgtacgaa gagctatcca
     1921 gcatgcggtc gagcaccgcg tctctgacat gggcccggtt gccgccgata cccgcattgc
     1981 gcgaatggaa aagcggcatg agaaaattct ccgcgtgcgg ataatcgccc gaccatttca
     2041 tcagatagat acccctggtt cccttgttgc tttcgcgcac catactcggc agatcgacaa
     2101 tgcggaattc gagatggatg ttaaactttt tgaatatata ccgcatgtat tccgcgatca
     2161 gcatgcgctc tttgtcgctg agggtgaaaa gaatcgctgt ctctgttttt acgggtatgc
     2221 ggtaatctgc tgccgccgga aacggctgtt gcggcggggg cgagagtatg gcgggtacag
     2281 ggtagtcagc cccggtgcag gcgcccagca gcgtgttttc gcacagggca ttgcggtcaa
     2341 acgcgaggtt gatcgcccgg cgcagagccg ggtcggcgag ggctgattcg ggcccgacat
     2401 taaaagccgc atagagcacg acgaggtcgt acatcgtcct gggctcatag agttcattgt
     2461 cgtgcgcgag tcgcctgtgg ataccgaggg cctcgtaggc gtcgagccgg ccttcacgga
     2521 aggcaaagta acgggcagtc tcgtccggca ttgccacgaa ttccacaggg ggagtgccat
     2581 cggttgtaga aagtaatatg cggtcgccgg tttgctccgc cacgcggtag cgggaaaaag
     2641 gaataaactc ccttgcagcc tggtgccgat cggctcgaaa tattgcacac tgtggcagcg
     2701 tcagcttgat gcgcgccagc tgccagtcga ctgctgctgg cctgcccgag ggcgccagct
     2761 cgatctcgac ggtgcggccg ttgaccttgc gcacggcacg tatctgctcc atgacccagc
     2821 tttggttggc gtccttttgc acgcgcccga gtgcataaac aacgtcatca gccgcagcct
     2881 cgccaggttt caacgtcaca aaaacgctac gtcccttcag ggatgagtca gccgccaggt
     2941 cttgttcgag cttcccttcg ggagagtaga cgaaaagtcg cctgtgcaga aaatttacaa
     3001 cgaggactgt tgattcatca acagcctgca acggatcaag aacttcggcc cggtatttga
     3061 gtggaatact aactaacgaa ttttgctttc cgggcttatc acagcccaac aagagccccg
     3121 cgacacagag aagtgcaatt aagtttcgca gagtcaaccc ccgtggtagt actgcattgt
     3181 ctgcatggga ttgaaaattg agcgggaaat gggatttgaa cccacgaccc tctccttggc
     3241 aaggagatgc tctaccactg agctattccc
//
LOCUS       DAOQJF010000368         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_19505_length_3233_cov_4.120516, whole genome shotgun sequence.
ACCESSION   DAOQJF010000368 DAOQJF010000000
VERSION     DAOQJF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19505_length_3233_cov_4.120516"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLF85_15085"
     CDS             <1..759
                     /locus_tag="PLF85_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21043.1"
                     /translation="FASDNILRPNEAIYKGSGVTLAEHSSAYTFEPTLRAEWEIPAER
                     YSWLSILWSLEGGYSPAREILSAAGSFRYQNAQAANVALTDLTYSGSLSVTEQRFNLT
                     PMFGLGAEYTNGWLQGLQETHLLARLSVGAMFASGQRHYVLSLAPQYVSAVNDTYVIQ
                     SEITQSHTMSVLPAARAELGARARLSSRLHIALLFSVTAVYGNIGWESQGYFAERAGL
                     SADKNIYQKVVSGIADQAYLGLAPAVFLALSTEL"
     gene            900..1928
                     /locus_tag="PLF85_15090"
     CDS             900..1928
                     /locus_tag="PLF85_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21044.1"
                     /translation="MKKRIDAKGNLITEEALVEDLYNETNIGSIAQLDPRANRAFFTH
                     TWYIKASTTVNYFNLGSEFQGVIKQIEGAFGGDALNMKLLNVNADFSMSTGQKVQRAP
                     TYFLPTGNLALGFYFGKHQVELEFGLAGLVPLKTIDLDTTMTLRDQTAGCSGTACQMA
                     NLGFVDPTSRTGQYSFRMTMNEDVWILTPSLAYDYVFLTRKWGRMGIGGAAGAMILST
                     SQQIAFKAVRTDPISPTPPEKPLEARVLQGSANSTNVTDIGPIFRLHVTYRPPPMTKF
                     WNTQMEVRLGANYGFVYLNRDVDGVGQAVLGDTLYGTFPLTSLGFKSKETNKFEMLGG
                     FIQVGIVF"
     gene            2021..3097
                     /locus_tag="PLF85_15095"
     CDS             2021..3097
                     /locus_tag="PLF85_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21045.1"
                     /translation="MLSPSKHDQIFAQTLKPAAEGSAPAEVSPAGPMDFPAPGEAQGI
                     GKMPKGDGALAQIRKKYFYLGELTWRGGFVMAKETSDWGESRKNSIEQDIFRQASTSN
                     NSKNVSVPASQNSAMNYMVGIEGLLGVNIDQLPGLRNAQWAKDKKFLRIGFHGYMAPL
                     YKNTTQTVAGDFAYYDALALTPTRTYPGTVQVDESLLTFSPGMSFFYWYERGFFDHKL
                     MPYAGFEVGLSIVYGKRKYSMNAGTFSTTEIEGGTPTQKTYSVEANLQESIINDFGFR
                     FMPAIGFQYHIKSGHYLDVRIGYQIQEYNVTLNRRGSLTETKKDAAGNFAVTWSEDFL
                     VKTESRKLSQSGIVILLGYTAGLF"
BASE COUNT          708 a          874 c          890 g          761 t
ORIGIN      
        1 tttgcgagcg ataacatact gcgcccgaac gaagcgatct ataagggcag cggcgttacg
       61 ctggcagagc acagtagtgc atataccttc gaacccacgc tgcgcgccga gtgggaaatt
      121 cctgccgagc gttacagctg gcttagtatt ctctggtccc ttgagggcgg ttattcgcct
      181 gcccgcgaaa tactctctgc tgccggcagt tttcgctacc agaatgcgca ggctgccaat
      241 gtggcactga cagatctgac ctattccggc agcctaagcg taaccgagca acgctttaac
      301 cttaccccca tgtttggcct cggtgcagag tacaccaacg gttggctgca ggggttgcaa
      361 gagacgcacc tgctggcgcg cctttcggtc ggtgccatgt ttgccagcgg ccagcgccac
      421 tacgttcttt cactggcgcc acagtatgtc tctgcagtca atgacaccta tgtgatccag
      481 agcgagatta cgcagtcgca cacgatgtcc gtgttgcccg ctgcccgggc tgaattgggc
      541 gcgcgcgccc gcctttcttc gaggctgcat attgcgctgt tgttttcggt taccgctgtc
      601 tacggcaata tcggctggga aagccagggg tatttcgccg agcgagccgg gctgtctgct
      661 gataagaata tttaccagaa agtagtttcc ggtatagcgg atcaggccta tcttggcctt
      721 gcccctgctg tttttttggc actctcgact gagttatgag catagtaaat aaacacgtgt
      781 ttactaatct gatccggctt ctgtcggcag ccttcgttgc gtccgcgttg gtctgccttg
      841 ttatgcccgg gttgacctat gcccaggacg gcggaacaat tcagttaaag aaaaaaaccg
      901 tgaaaaagcg catcgatgcg aagggtaacc tgatcaccga agaggcgctg gtcgaagacc
      961 tctacaatga aaccaacatc gggtccattg cgcagcttga cccgcgtgcc aatcgcgcct
     1021 tctttactca tacctggtat atcaaagcct cgaccacggt gaactacttc aacctgggtt
     1081 ccgagttcca aggggttatc aagcagattg aaggtgcatt cggcggagat gcgctgaaca
     1141 tgaaactgct gaacgtgaac gccgattttt cgatgagtac gggtcagaaa gtgcagcgtg
     1201 cgccaactta ctttctgcca acgggtaacc tggcgctcgg tttctatttt ggtaagcacc
     1261 aggtggagct cgaattcggg ctcgcgggtt tggtaccgct caagactatc gacctcgata
     1321 cgacaatgac gctgcgcgac cagactgccg gttgttctgg tacagcatgc cagatggcaa
     1381 atcttggttt tgtcgacccg acttcacgca cggggcagta ctcgtttcgc atgacaatga
     1441 atgaagatgt ttggattctt actccttcgc tcgcctatga ctatgtcttt ctcacgcgca
     1501 aatggggacg catgggtatt ggtggtgcgg caggcgcgat gattctctcg acttcgcagc
     1561 agattgcctt taaggccgtc cgtacagacc ctatctcgcc gacgccgccc gaaaagccgc
     1621 ttgaagcgcg tgtgctgcag ggcagcgcga attcaaccaa cgtgacggac atcggtccga
     1681 tttttcgttt gcacgtgacc tatcgcccgc cgccgatgac aaaattctgg aatacacaga
     1741 tggaagttcg cctgggtgcg aactatggtt ttgtatacct caaccgcgac gtggacggcg
     1801 tcgggcaagc agtgctcggc gacactttgt atggcacgtt cccgctcacg tcgctcggct
     1861 ttaagagcaa agagacgaac aaatttgaaa tgctgggtgg tttcatccag gtggggattg
     1921 tcttctgatg gatatccgtc gctcacgccc ccaccgcctt cggcacctcc cccatgtttg
     1981 ggggaggttg ggagggggcg ctgttgttgc agcgttcatc atgctgagcc catcgaagca
     2041 tgaccaaatc ttcgcgcaga cgcttaaacc cgcggcagaa ggtagcgctc ccgcagaggt
     2101 gtcacccgcc ggtccgatgg attttcctgc gccgggcgaa gcgcagggca taggcaaaat
     2161 gccaaaaggc gatggcgcac tggcgcagat tcgcaagaag tacttctatc tcggtgagct
     2221 gacctggcgt ggtggcttcg tgatggcaaa agaaacctct gactggggcg agagccggaa
     2281 aaattcgatc gagcaggata tatttcgcca ggcgagtacg tctaacaatt caaagaacgt
     2341 gagcgtacct gcatcacaga acagcgccat gaactacatg gtgggcattg aaggccttct
     2401 gggtgtaaat atagaccagc tgcctggcct gcgcaatgca cagtgggcga aagacaagaa
     2461 gttcctgcgc attggttttc acggttacat ggctccgctc tataagaaca ccacgcagac
     2521 tgtcgcggga gattttgcct actatgacgc gcttgccctg acgccgaccc gtacataccc
     2581 gggtacggta caggtcgacg aaagcctgct cacattctca cctggcatga gctttttcta
     2641 ctggtatgaa cgtggctttt ttgaccataa gctgatgcct tatgccggct ttgaagtagg
     2701 gctctccata gtgtatggca agcgtaagta ttcgatgaat gccggcacct tctcgaccac
     2761 cgagattgaa gggggaactc ccacccagaa gacctactcg gtagaagcca acctgcagga
     2821 gagcatcatc aacgattttg gctttcgttt tatgcccgcg attggtttcc aataccacat
     2881 caagagcggt cactacctcg acgtgcgcat cggctaccag atacaggaat acaatgtaac
     2941 gctcaaccgg cggggctctt taaccgagac caagaaagat gcggctggca attttgccgt
     3001 gacatggagc gaggattttc tggttaagac tgaaagtcgt aaactttcac agagtggtat
     3061 cgtgatactt ttgggttata cagcggggtt attctgatgc ttcggctacg ctcagcacaa
     3121 gtgcttcgac tgccctcagc acaggtgcaa atattgactc gcatacgctc gtctctgctc
     3181 gccatggcgc tgctgcccgt tctcgcggcc tgccttggtc gcgcgacacc gcc
//
LOCUS       DAOQJF010000369         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_19683_length_3215_cov_4.708544, whole genome shotgun sequence.
ACCESSION   DAOQJF010000369 DAOQJF010000000
VERSION     DAOQJF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19683_length_3215_cov_4.708544"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLF85_15100"
     CDS             complement(<1..210)
                     /locus_tag="PLF85_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21046.1"
                     /translation="MKKLHSRFIPVFDSIGGGVFSLLESVGAFAIFAGQTFRYCFVPP
                     FDFRNLLHQMGEIGVKSIPVGVVSSF"
     gene            complement(207..2855)
                     /locus_tag="PLF85_15105"
     CDS             complement(207..2855)
                     /locus_tag="PLF85_15105"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNE21047.1"
                     /translation="MSRIAALSLILIFTYEAYARAGGGGSYRSSSSSSSRSSSSSSSR
                     SSSSSSYRSSSSSSSSSYRSSSSSSSSRSYGGSSSSSYREPEYRQLPIDDLEAYSAEI
                     NLLENGRLEVREALKFKPYDKNPKHVYYRKLTSLHETQRILPGATVDGDLAHHSGPGV
                     DMLSFRSTAPASPLLSAAIRFEADRVYFESESGLWDFNFGLTKANLGSVRIRDLPPGA
                     TAWLFDQAIGGKLIGKIAGNGGPAILASAVKLHNPRLVITGAKAPAAEAKSVIPRFAQ
                     AKVSAILSADSTIEAEWQAVAPRGAWIMPVYNARNSYIGMAKNAAGGKIERYYPDAYL
                     EPQNPATPARFRVLHFEKYREAEYLQLPLGHISKTERGAEARHYASADNATYTAYTPL
                     VVELPTERPALAQIVLCKDNWRYSWGCEPVRTILSESSQQGNRLSVNLLEPVVNAEVM
                     LRIYLPGGTLTAPTFGKQLAHELYHFWKFGGKPAWAYWTYTLFILIASIVSVILFISF
                     TRRQKERKLALKRAIEQQKRENEILKSLREHDPDFNLEAFYRRGREIATRIQHSWSAG
                     DMRDCRRFLSQGVYNRFRLQLKLMRELEKRRNVMADFRINNFYVLAHSRSGDFDCLAV
                     RLEAAARDTWVAVDTSEADAKKAAGSAALQSFVEIYSFMRRSSAKTEKEQPLENCSHC
                     GTPFSSEGETNKCRSCGAVMGSGTFDWVLAEITQLSEYRGVQSPKNLPAGLSPDRIED
                     RASFIFWRDVMTRSKGDLNYVARDATSTYLAAPPKKQLLYDIAVGAADLKSVESGEQG
                     LKAKVMIKWSAADGKDKVVRHRRSWLHLVVRDVDAKSQTFADPGCPSCGAPLPETDSE
                     KCSYCQSTIARKNADWLLESIETTVE"
     gene            complement(2902..>3215)
                     /locus_tag="PLF85_15110"
     CDS             complement(2902..>3215)
                     /locus_tag="PLF85_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21048.1"
                     /translation="VAHRWNNNWHTLLYTGPRHTAAPESYTSWENNLSFHYMLSGIRH
                     FIGLEMNQEIANRRSTITFRPQIRLQLSHNLLIGLVAGLPDNMRRNGMSTFVRHIYEL
                     S"
BASE COUNT          732 a          846 c          887 g          750 t
ORIGIN      
        1 gaaagaactc acgacaccga caggtatcga ctttacgcca atttcgccca tctggtgcaa
       61 taggttacga aaatcaaacg gaggaacaaa acagtaccgg aaggtctggc cggcgaaaat
      121 ggcaaaagcg cccacagact cgagcagaga aaacactccg ccgccgatgc tgtcaaatac
      181 cgggataaat ctgctatgca gttttttcat tcgacggtcg tctcaatcga ttcgagtagc
      241 cagtctgcat ttttgcgtgc tatcgtcgac tgacaatacg aacacttttc actgtcggtc
      301 tcggggagag gggccccgca ggaaggacag cctgggtcgg cgaaggtttg gctctttgcg
      361 tctacgtctc tgacaaccaa atgcagccat gatctgcggt ggcggaccac tttatctttg
      421 ccgtctgcgg cagaccattt gatcatgacc tttgctttca gtccctgctc acctgattcc
      481 acagatttga ggtctgctgc gcccacggca atatcgtaaa ggagctgctt ttttgggggg
      541 gctgcaaggt aggtgctggt agcatcccgt gccacgtaat tcagatcgcc tttactgcgg
      601 gtcatgacat cccgccagaa gatgaatgac gcccggtctt caatgcgatc aggacttaaa
      661 ccagcgggaa ggtttttggg gctttgaaca ccgcgatact cgctgagctg cgtgatttct
      721 gccagaaccc agtcaaaggt gccggatccc atgacggcgc cacagcttcg gcacttgttg
      781 gtttcccctt cgctcgaaaa cggcgtacca cagtggctgc agttttcgag cggctgttct
      841 ttttcggtct tcgcagaaga tcttcgcata aacgagtaga tttcgacaaa actctgcaac
      901 gcggcggagc cggcggcctt tttggcatcc gcctcggagg tatcgacggc tacccaggta
      961 tcccgtgctg ccgcctcgag cctgaccgcc aggcaatcga aatcccccga gcggctatgg
     1021 gccagcacat aaaaattatt gatccggaag tcagccatca cgttacgccg cttttcgagc
     1081 tcgcgcataa gctttagctg caggcgaaat cggttgtata ccccttgcga caaaaaacgc
     1141 ctgcaatcgc gcatgtcacc tgcgctccag ctgtgctgta tacgcgtggc aatctcccgt
     1201 ccgcggcggt aaaatgcttc gagattaaag tcaggatcgt gctcgcgcag agatttcagg
     1261 atttcatttt cccgcttctg ctgctctatt gcgcgcttca gggcgagctt tctttctttt
     1321 tggcggcggg tgaagctgat aaacaggata accgagacga tcgatgcaat caggataaac
     1381 agcgtataag tccagtaagc ccaggcgggc tttccgccaa atttccagaa atggtatagt
     1441 tcatgcgcaa gttgtttgcc aaatgttggt gcggtcaatg tgccgcctgg caggtagatt
     1501 cgcagcatga cttctgcatt gacgactggt tcaagcaggt tgacactcag gcgattgccc
     1561 tgttgtgaag actcagaaag aatcgtgcgc accggttcac atccccacga atagcgccag
     1621 ttatccttgc agagaacaat ctgcgcgagg gccggtcttt ccgttggcag ctcaaccacc
     1681 agtggagtgt aagcggtata agtggcattg tcagccgagg cgtagtgcct cgcctctgcc
     1741 cctcgctcgg ttttgctgat gtggcccaat ggaagctgca ggtattcggc ttcacggtat
     1801 ttttcaaagt gcaaaacccg gaaccgcgcg ggtgtagcag ggttctgtgg ttccagatac
     1861 gcgtccggat aatagcgttc aatcttacca ccggccgcat tttttgccat gccaatgtag
     1921 ctattccgcg cattatagac cggcatgatc catgcgcctc ttggggccac tgcctgccat
     1981 tctgcttcga tagtggaatc tgctgacaga atggcgctga ctttcgcctg ggcaaatcgc
     2041 gggatgacag attttgcctc ggccgctggc gccttagcgc cggtaatcac caggcgtggg
     2101 ttatgcaact tcaccgcgga cgccagaatc gccgggccac cgttgcccgc aattttgccg
     2161 atcagtttgc caccaatagc ctggtcaaag agccatgcag tggcgcctgg tggtaagtca
     2221 cggattctga cggaacccag attagcctta gtcagtccga aattaaaatc ccataaaccg
     2281 ctttcgctct caaaatagac gcggtcagct tcgaaacgaa ttgccgccga aagtaaagga
     2341 ctcgcaggcg cagtcgatct gaagctgagc atatctacac ccgggccaga atggtgcgcc
     2401 agatcgccgt caacggttgc accaggcaag atacgctgcg tctcgtgcag cgacgtcagt
     2461 ttacggtaat agacatgttt ggggtttttg tcgtagggtt taaatttgag tgcttcgcga
     2521 acttcgagcc tgccgttttc cagcaggtta atttctgccg aatatgcctc gaggtcgtca
     2581 atcggtagct gccggtattc gggctcacgg taagatgagc tggaactgcc gccgtaagag
     2641 cgcgaggacg atgacgaact ggaagagcgg tacgacgacg agctcgagga tgaggacgat
     2701 ctgtaggatg aactagaaga cgacctgctt gaagatgaac tcgatgaacg ggaggagctg
     2761 ctgctcgaag accggtagct accaccgccg ccggcgcggg cgtatgcctc gtatgtaaaa
     2821 atgagaatga gagaaagggc agctatccta gacacagctg attcctgatt tcgccggcgg
     2881 tatgtaaaga gaatatggtg cttaactcag ctcatagata tgccgcacaa acgtactcat
     2941 gccattgcgc cgcatgttgt ccggcaaacc agcaactaag ccgatcagca ggttgtgcga
     3001 aagttgcagc ctgatctgcg gccgaaaggt aatcgtgctg cgtctattcg caatctcctg
     3061 gttcatctct aaaccgatga aatgccggat gcctgagagc atgtaatgaa aactgaggtt
     3121 gttctcccag ctggtgtatg actctggtgc agccgtgtgt cgcgggccag tatagagcag
     3181 ggtatgccag ttgttgttcc agcggtgggc gacga
//
LOCUS       DAOQJF010000370         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_20096_length_3174_cov_5.111895, whole genome shotgun sequence.
ACCESSION   DAOQJF010000370 DAOQJF010000000
VERSION     DAOQJF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20096_length_3174_cov_5.111895"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLF85_15115"
     CDS             complement(<1..623)
                     /locus_tag="PLF85_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21049.1"
                     /translation="MQSRAKILSLLSLVIVLPISGYTYQTRSGDNDLDFKFDVPAGWE
                     KMVGKRRSSAYASFYKDDPAGKVAIEVYSYRADNANLELLLLQQRARLAVVFDRVLLQ
                     QTQSTPNREDIRKHEWSAWLGKTRYRLVTSFLRTDDRVLKIFCVAPAHRYGAYAPVFE
                     NAVLSFSFSDGKHSAGDALVSKPWQPEEKKFPVPLAQIKKEKPAAEK"
     gene            786..2051
                     /locus_tag="PLF85_15120"
     CDS             786..2051
                     /locus_tag="PLF85_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE21050.1"
                     /translation="MSGISRAAKAAILVSALGYFVDIYDLLLFGIVRIPSLRTLGVPD
                     AAMKEIGASLLNYQMFGMLLGGIFWGVLGDKKGRLSVLFGSIFLYSVANLANAFVDRT
                     GSMETGLLAYQWLRLIAGIGLAGELGAGITLVSELMPADKRGYGTMVVAGFGILGAVV
                     ATLIGDIFPWQTAYIIGGVMGLMLLIMRIGVYESGMYEQTAEAGVRRGDFLYLFTSGE
                     RFMRYLRSILIGVPLWFAVGILIIFSKEFGQALGATGPINPGYAIGFMYGGLSAGDFL
                     TGYLSQRLGSRRMVVLVCQIINLVLIAVYLLSRNFTPTYFYILCVILGLANGYWAVFV
                     TIAAEQFGTNLRATVATTVPNFVRGSVPLLTTAFLYLDKEVFAKGLSNSEQGMVPAAA
                     IVGGVSLVLGIWATLTMPETHGKDLNFHE"
     gene            2057..2383
                     /locus_tag="PLF85_15125"
     CDS             2057..2383
                     /locus_tag="PLF85_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNE21051.1"
                     /translation="MDWFLVIVAGLFEVGFASCLGQAKTATGNAVLGWYAGFTLCLIV
                     SMGLLMKAVETLPIGTAYAVWTGIGALGTVIVGILFLREPADFWRLFFLSTLVLSIVG
                     LKSVSH"
     gene            complement(2380..2868)
                     /locus_tag="PLF85_15130"
     CDS             complement(2380..2868)
                     /locus_tag="PLF85_15130"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNE21052.1"
                     /translation="MAFLSELRLVEMPARQLAGQRVRMSLVQNRSAELWQAFRPLATQ
                     VSGRIGGDFFSVQVYDGVEYFTAFRPDRDFTRWAAVEITEGSALPAGLEILELPAGQY
                     AVFDYRGTPAEFAAYAEQIYGRHLPQAGFRLDNRPHFEILGPAYRPNDSNAEETIWIP
                     VC"
     gene            complement(2881..>3174)
                     /locus_tag="PLF85_15135"
     CDS             complement(2881..>3174)
                     /locus_tag="PLF85_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNE21053.1"
                     /translation="IAAATAEEWNKVHNPKDRGVPQDMPEFRYTQGAQLVNVVKDAGL
                     APSNSEARRMIEQKSIYRVEADGSEQVLEDFKMPVNEGFILRAGKRKFVKLID"
BASE COUNT          656 a          888 c          851 g          779 t
ORIGIN      
        1 ttcttctcag ctgcaggttt ttcctttttt atttgcgcta aaggcacggg aaattttttt
       61 tcttctggct gccagggctt actcaccagc gcatcgcccg cactgtgctt gccatctgaa
      121 aaagaaaagg atagcacagc gttctcaaat accggggcat aagcaccata acgatgcgcc
      181 ggtgcaacgc agaagatctt gagcacgcgg tcatcggtac ggagaaatga agtcaccagg
      241 cggtaccggg ttttgccgag ccaggcgctc cactcatgtt tgcgaatatc ttcgcggttg
      301 ggtgtgctct gcgtctgctg caacaagaca cggtcgaaaa ctaccgcaag ccgggcacgc
      361 tgctgtagca gtaaaagttc gaggtttgca ttatccgcac ggtaagaata gacttcaatc
      421 gcaacctttc ccgccgggtc atctttatag aacgaagcat aggcggagct tctgcgttta
      481 ccgaccattt tctcccaacc ggccggcaca tcgaacttga agtcgaggtc gttatcgcca
      541 gaccgggtct ggtaagtata gcctgagatt ggcagcacga taactaatga cagcagtgac
      601 agtattttgg cacgtgactg cattagtgaa cctatgttca ctttggcggc aaaacacccc
      661 gggacaagcg aaaactaaat actgcagggt attctatcgc ctggcgcccg ctctggaaat
      721 cgacaaacta taaaagagct aacatttgcc cttttcgcac tgtctcatct ctgcagcgct
      781 tccgcatgtc cggtatctcc cgcgccgcaa aggccgcgat tcttgtttcg gcgctcggct
      841 atttcgtcga catctacgac ctgttgcttt tcggcatcgt cagaattccc agcctgcgga
      901 cactcggcgt gcccgatgca gcgatgaaag aaattggcgc ttcactactc aactaccaga
      961 tgttcggcat gctactcggc ggcatcttct ggggcgtgct cggcgacaag aagggccgcc
     1021 tttcggttct cttcggttcg atcttcctgt actcggtggc aaacctcgcg aatgcgtttg
     1081 tcgaccgcac aggttcaatg gaaaccggac tgctcgccta ccagtggctg cgtctgattg
     1141 cgggtattgg gctcgcgggt gaactcggcg ccggtatcac gctagtgagc gaactcatgc
     1201 ccgccgacaa gcgcggttat ggtacgatgg ttgtggcggg tttcggtatt ttaggcgcag
     1261 tcgtggcaac actgatcgga gatattttcc cttggcagac cgcctacatt atcggtggag
     1321 tgatgggact catgctgctg atcatgcgta tcggtgtcta tgaatcaggt atgtacgaac
     1381 aaacagccga agcgggagtg cgccggggcg acttcctgta cctcttcact tcaggcgaac
     1441 gtttcatgcg ctacctgcgt tcgatcttaa tcggcgtgcc gctctggttt gctgtcggca
     1501 tcctgattat cttctcgaaa gaatttgggc aggcgctcgg cgcaactgga ccaattaacc
     1561 ccggttatgc gatcggtttc atgtacggag ggctctctgc cggtgatttt cttacgggtt
     1621 acctgagcca gcgcctcggt agccgccgca tggtggtgct ggtctgccag atcattaacc
     1681 tggtgctgat cgcggtctac ctgctctcgc gcaatttcac gccgacctat ttctacatac
     1741 tctgcgtgat tctgggattg gcgaacggct actgggcggt attcgttact atcgcggccg
     1801 aacagtttgg caccaacctg cgtgcgaccg tcgcgacgac agtaccgaac tttgtgcgcg
     1861 gttccgttcc gctgctaacc actgcgtttc tttacctcga caaagaagtt tttgcaaaag
     1921 gcctcagtaa tagtgagcag ggtatggtac ctgcagctgc catcgtgggc ggagtttcac
     1981 tggtactggg catctgggcg acattgacca tgcccgaaac acacggcaaa gatctcaact
     2041 tccacgaata accccattgg actggtttct tgtcatcgtt gccggcctgt ttgaggtcgg
     2101 ctttgcctcc tgcctgggcc aggccaaaac cgcaaccggc aacgccgtac tgggctggta
     2161 tgccggtttt accctctgcc tcatcgtcag catggggctg ctaatgaagg cagtggaaac
     2221 cctgcccatc ggcacggctt atgccgtatg gactggtatc ggcgcactcg gcacggtaat
     2281 cgtcggcatc ttgtttctca gggagccagc tgacttctgg cgattattct ttctctcaac
     2341 gctcgttttg tctatcgtcg gactgaaaag cgtatcgcac taacagaccg gaatccagat
     2401 cgtctcttcg gcattggagt cattgggccg gtatgccggc ccgagtattt caaaatgtgg
     2461 ccggttatcc agcctgaagc ctgcctgcgg cagatgccgg ccatagatct gttcagcgta
     2521 ggccgcaaat tctgcaggtg tgccgcggta atcaaatacc gcgtattgcc ccgcaggcaa
     2581 ttccagaatc tcaagaccgg ccggtagcgc gcttccttcg gttatttcga ccgcagccca
     2641 gcgggtaaaa tcgcggtctg gcctaaatgc ggtaaaatat tccacaccgt cgtagacctg
     2701 aaccgagaaa aaatccccac cgatgcgacc ggaaacctgc gttgccaaag gcctgaaagc
     2761 ctgccagagt tcggcgctgc ggttttgcac gagcgacatt cgcacgcgct gacctgcaag
     2821 ctggcgcgcg ggcatctcga caagtcgcaa ttcagaaaga aaagccatga gtgcggtttt
     2881 ttagtctatc agcttcacaa acttgcgttt tcccgcgcgc aggataaaac cttcgttgac
     2941 tggcatcttg aaatcctcaa gaacctgctc actgccgtcg gcttcaaccc tgtagataga
     3001 tttctgctcg atcatgcgac gggcctcaga attcgacggt gcaagccccg catctttgac
     3061 gacgttcacc agctgcgcgc cctgcgtata gcgaaactct ggcatgtcct gcggtacgcc
     3121 gcggtctttg ggattatgga ccttgttcca ctcttctgcc gtcgcggcgg caat
//
LOCUS       DAOQJF010000371         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_20322_length_3154_cov_4.290094, whole genome shotgun sequence.
ACCESSION   DAOQJF010000371 DAOQJF010000000
VERSION     DAOQJF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20322_length_3154_cov_4.290094"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /gene="pyk"
                     /locus_tag="PLF85_15140"
     CDS             complement(<1..1099)
                     /gene="pyk"
                     /locus_tag="PLF85_15140"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNE21054.1"
                     /translation="MPYRKTKIICTIGPATNTLDKIRALAEAGMNIARINMSHGDQKS
                     HQDVIQRIKSYNKTAAMPIAILLDTQGPEIRTGDVMENLTLHVGDVIEITAGSENTES
                     KSVITVNYRDMLRDLKPGDRITLDNGIINLDVVEKTDFGLRCKVVDGGIVKSRRHINL
                     PGIRVNLPSITAKDVQDIMFGIKHDVDFIALSFVRTAADVEECKRMIERYKGHAQVIA
                     KIEDSQAVQNYKEIISAAHGVMVARGDLGVEVPIEELPIIQRRIIKECAHQGKRVIVA
                     THLLESMIENPIPTRAEVTDVANAAYEEVDAVMLSGETAAGKFPIKAAETLNKILKRI
                     EITGGIGWSRDFKSDDVKGAFAESAVELADQL"
     gene            1234..2148
                     /locus_tag="PLF85_15145"
     CDS             1234..2148
                     /locus_tag="PLF85_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21055.1"
                     /translation="MEDLYTLTPNRSVEAQELNRMVRLLAQAGKTHFKQYLYDPLYTA
                     GWERKAGEARKILEHLRGELKNPAYRHTLAPNCKRMVGTAMAENYSTLGDCCLFFLEV
                     IQMLPQIGEAPEAAEFMRVLKGPLAEWQKIQDERSAYMFDRELNNLADAQIAEAFQPV
                     KLGVTEERARITAEIQRLYQQILVASRADNFKRAAQLLARYMIEYSDAENYAEKDIRK
                     VIEALQLRDKTFEASLNAMLATDLYYRITQGIMKGDLKVAVRMIRKYAHIFEGNPNIR
                     YYYEIDRLERMLYNVITKKDLWKVMKKA"
     gene            2174..2521
                     /locus_tag="PLF85_15150"
     CDS             2174..2521
                     /locus_tag="PLF85_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE21056.1"
                     /translation="MADAQISISDKVNGKKAVVSIAGELDAKTTPALKAKLDELLGKG
                     IDGLVVDCTGLTYVASAGIGALNAALKALKDKNGKMALSGLSKEVRDTMDLMFFTKRV
                     AVFATAAEGEKNI"
     gene            2524..2982
                     /locus_tag="PLF85_15155"
     CDS             2524..2982
                     /locus_tag="PLF85_15155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21057.1"
                     /translation="MRTEIALKISGDLGELKSVREAVRAFLDSTKHIDLGEDEKGGLD
                     VIAQSRIILAIDEAVANIIEHGFPDEATLAGAQISLTMRKEPQKVEFEIRDNGIAFNP
                     LEKKSDTELYYEEGLENGVGLLALMTLDCHYERAGEQNVLLLRRDWGNKK"
BASE COUNT          716 a          832 c          888 g          718 t
ORIGIN      
        1 tgagctggtc ggcaagttca accgccgatt cggcaaaggc tcctttgacg tcgtctgatt
       61 tgaagtcgcg tgaccagccg atgcccccgg tgatttcgat gcgcttgaga atcttgttga
      121 gagtttccgc ggctttgatc ggaaattttc cagcggcagt ttcgcccgaa agcattacgg
      181 catcgacctc ttcgtacgca gcgtttgcca cgtcggtcac ctcggcgcgc gtcggtatcg
      241 ggttttcaat catcgactcg agcaggtgcg ttgcaacaat gacccgctta ccctggtgag
      301 cgcattcttt gataatgcgc cgctgtataa tcggcagctc ttcaatgggc acttcgacgc
      361 ccaggtcgcc gcgcgccacc atgaccccgt gcgctgcgga tatgatttcc ttgtaattct
      421 gcacagcctg cgaatcttca attttggcga tcacctgcgc atggcctttg tagcgctcga
      481 tcatgcgctt gcactcttcg acatctgccg cagtgcgcac aaaagacagc gcaatgaagt
      541 ctacgtcgtg cttgatgccg aacatgatgt cctgcacgtc cttggccgtg atggaaggca
      601 ggtttaccct gatgccgggc aggttgatgt ggcggcgtga tttgacgatg ccgccgtcga
      661 ccactttgca gcgcaggccg aaatcagttt tttcgacgac gtccaggtta atgataccgt
      721 tatcgagtgt gatgcggtca ccgggcttga ggtcgcgcag catgtcccgg tagttgaccg
      781 tgatgacgct cttgctctcg gtattttctg agcccgcggt gatttcgatc acgtcgccca
      841 cgtgtagggt caggttttcc atgacgtcgc ccgtgcgaat ctccgggccc tgtgtatcga
      901 gcaatatggc gattggcatc gcggcagtct tgttgtacga cttaatccgc tgtatgacgt
      961 cctggtgcga cttctggtcg ccgtgcgaca tgttgatacg cgcgatgttc attcccgcct
     1021 cggcaagcgc cctgatcttg tcgagcgtgt tggtggccgg gccaatcgtg cagataatct
     1081 tggttttgcg gtatggcata gtgaagactg taccgaaatc taactccggt caggctgtcc
     1141 ggcgaattaa tcaggggctc cgttgtccgg gtgctgcaga ttcctttacc ggtacatggc
     1201 gctctgtgga aagtccctta gcctgtaacc gccatggaag acctttatac cctcacgccc
     1261 aaccgatctg ttgaagcgca ggaactcaac cgtatggttc gcctgctggc gcaggcgggt
     1321 aagacccact ttaaacagta cctatacgac cccctctata ccgccgggtg ggagcgcaaa
     1381 gcaggcgaag cccgcaagat tctcgaacac ctgcgcggcg agctcaagaa cccggcctac
     1441 cggcatactc tggcgcccaa ctgcaagcgc atggtgggta ccgcgatggc tgagaactac
     1501 tcgaccctgg gcgactgctg tctcttcttt ctcgaggtga tacagatgct gccgcaaatc
     1561 ggtgaagctc ccgaagcggc ggagtttatg cgtgtactca aggggcctct ggcagagtgg
     1621 cagaaaatcc aggatgagcg cagcgcgtac atgttcgacc gcgagctcaa taacctggcc
     1681 gacgcgcaga ttgccgaagc ttttcaaccg gtaaaactcg gcgtcaccga agagcgcgcg
     1741 cgcattacgg ccgagataca gcgtctttac cagcagatac tggttgcctc gcgggcagac
     1801 aacttcaagc gcgcggccca gctgcttgcg cgctacatga ttgagtacag cgatgcagaa
     1861 aattatgcag aaaaagatat tcgcaaggtg atcgaagcgc tgcagctgcg ggacaaaact
     1921 ttcgaggcct cgctcaacgc gatgctggct actgatctct actaccgcat cacgcagggt
     1981 atcatgaagg gcgatctcaa ggttgccgtg cgcatgatac gcaagtacgc acatatcttc
     2041 gaaggtaacc caaatatacg gtactattat gaaattgacc gcctcgaacg tatgctctac
     2101 aatgtcatta cgaagaaaga tctctggaag gtcatgaaga aggcctgaga cacgaagact
     2161 aaagaggaaa cagatggcag atgcacagat ttcaatcagc gacaaggtga atggcaaaaa
     2221 ggcggtagtt tctatcgcgg gggagctcga tgccaagacg accccggctc tcaaggcgaa
     2281 gctcgacgaa ttactgggta aaggtatcga cggcctggta gtggactgca ccggtctcac
     2341 gtatgtggct tctgccggta tcggcgcgct caatgcggcg ctcaaggctc ttaaagacaa
     2401 gaacggcaaa atggcgcttt caggcctcag caaagaggtg cgcgacacca tggatctcat
     2461 gttttttacc aagcgcgtgg cggtctttgc tacggcggca gaaggcgaaa agaatatctg
     2521 attttgcgta ccgagatcgc actcaaaatt tcgggcgacc tgggggagct gaaaagcgtg
     2581 cgcgaagcag tgcgcgcttt tcttgattcg acgaaacata tcgacctggg tgaagacgaa
     2641 aagggcggtc tcgatgttat tgcgcaaagc cgtattatac ttgcgattga cgaagctgtc
     2701 gcgaatatta tcgagcacgg ctttcccgat gaggcaacgc tcgccggagc gcaaatatcg
     2761 ctcaccatgc gcaaagaacc gcagaaggta gagtttgaaa ttagggataa cggtattgct
     2821 ttcaacccgc tcgagaaaaa aagcgatacc gagctgtatt acgaagaagg gctcgagaac
     2881 ggcgtggggt tactggcctt gatgaccctc gactgccatt acgaacgcgc cggcgaacag
     2941 aacgtcctgt tgctgcgccg cgactgggga aacaaaaaat gaaaaaggtc tggcaaaaag
     3001 ttttaccaat gatttcgccg gggatcaggc ttcagctcac tctctttacg attttcttcg
     3061 tcagcgcgtt aatagtggca agttttgtgt tctcctattt cagccagaag gctgaccttg
     3121 aagagtcgtt tcgtaaagag gtccgcgttc ccga
//
LOCUS       DAOQJF010000372         3119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_20716_length_3119_cov_4.484334, whole genome shotgun sequence.
ACCESSION   DAOQJF010000372 DAOQJF010000000
VERSION     DAOQJF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20716_length_3119_cov_4.484334"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..478
                     /locus_tag="PLF85_15160"
     CDS             272..478
                     /locus_tag="PLF85_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNE21058.1"
                     /translation="MPIVKVSPKFQVVIPKEIRENSGIKAGSKMDIFVYDGRIELVPI
                     KSIKTLKGSLPGIDTHIQRDSDRI"
     gene            475..846
                     /locus_tag="PLF85_15165"
     CDS             475..846
                     /locus_tag="PLF85_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE21059.1"
                     /translation="MNIVDSSGWLEYLAGSKNGDIFGKYIENTDRLIVPVITIYEVFK
                     KILTESNEENALKIAAHMRLGRVIDIDLSLSLEAAKFGKQLKLPMADALIYATARKYD
                     CTIWTQDEHFKGLEGVQYFPK"
     gene            complement(945..1793)
                     /locus_tag="PLF85_15170"
     CDS             complement(945..1793)
                     /locus_tag="PLF85_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNE21060.1"
                     /translation="MLRGIYTGASGMAAMQVKMDTVANNLANVDKTAFKEDVTIFRAN
                     PEVLIHRTRDDGVGWVPMGGFDISPLVGKLGTGAEVNEIYTRFDQGALKRTERDQDLA
                     INGEGFFVVQSQAGPRMTRSGSFILDRNGMLVTPDGFPLMGEKGPVQVNHNNYIIKSN
                     GEVWINQAVGTDPRNMTSKDVNQWENPVLLDTLQIRTVDHPRHLDKVGQSFYNTTPES
                     GDMRPVDQVKAPEILQGFLEASNVNIVTEMVNMIEVQRMYEANQRAITTHDGMLGTLI
                     NQVLRV"
     gene            2056..2712
                     /locus_tag="PLF85_15175"
     CDS             2056..2712
                     /locus_tag="PLF85_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21061.1"
                     /translation="MQPASFKARPGKDFALQVGVYESVTRAEVIALRADLPPGNQGAK
                     VSDQLVTDVSQQMHEKLEQQRKQRLKEIEAEKKRVAEMRAKNPDYDSAAAPGDPQAKV
                     TLKRAITTGWPTKSREIAFLGGFERVSGGGQSSSGTSFLALRFFPGDKAAVRLGVFGG
                     ASLIPGPKTSDWGGVPPAAGVSFLIVRNLALEVTVDAAYYSSVSRMGYGVGYGITIPF
                     "
     gene            2780..>3119
                     /locus_tag="PLF85_15180"
     CDS             2780..>3119
                     /locus_tag="PLF85_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21062.1"
                     /translation="MQTKQDLRSSETEPKAHSWFSNPLDWSHIDRLILLAGMVFLVPL
                     FFGGVLALTLLLAPEWLNSRVAIPMFALHTGYLTIILMFVLIALKKRRSISTWPLFED
                     VIIYSYVAVVF"
BASE COUNT          719 a          789 c          833 g          778 t
ORIGIN      
        1 atgttttcaa ctcgtagcgg cgatttgctg tcgtcgagaa cgcagctccg ctttgcggtt
       61 tgtcagtaaa ttaagcagcc tttgaacagc tttcgcttgg cagcgcccaa cgataactgt
      121 tctctaaacc gaaatggcgg gtacgcacga gctgtgcgcc ctggactacg caccgaatac
      181 agagactacg ctacaaacct cgtgtgaatt aaccttgacg aattatcgat ttagtaattg
      241 taatacccga ttgctatcga taggagagat tatgcccatt gttaaggttt cgccaaaatt
      301 tcaggtcgtc attccgaagg aaatcagaga aaattcagga attaaagctg gctcaaagat
      361 ggatattttt gtatacgatg gccgaattga gttagtccca attaaatcca taaagacttt
      421 aaagggtagt ctgccgggca tcgataccca tattcaacga gattccgatc gcatatgaat
      481 attgtcgact cttcaggttg gcttgaatat cttgctggca gcaaaaatgg cgacatattt
      541 ggcaagtata tcgaaaatac cgacaggcta atagtgccgg tcattacaat ttatgaagtg
      601 ttcaaaaaaa tactcaccga atcaaatgag gaaaatgccc tgaagattgc cgcacatatg
      661 cgactcggaa gagtcatcga catagatctt tccctgtcgc tcgaagcagc caaattcggg
      721 aaacagctca agctcccaat ggcagatgca ttaatttatg caacggcgcg caagtacgat
      781 tgcaccattt ggacacaaga cgagcacttt aaaggtctcg agggtgttca gtatttccca
      841 aaataagata cccagctgcg gcatcgcact tgtgcgctga atgcacagct gttccgtgca
      901 aatcatcgtg cacggtatcc ctggtaccgg aaacctaaga aattctacac ccgcagcacc
      961 tggttgatca gcgtaccgag catgccgtcg tgcgtggtga tggcgcgctg gttcgcctcg
     1021 tacatgcgct gcacctcgat catgttgacc atttcggtga caatgttgac gttcgaggct
     1081 tcgagaaagc cctgcagaat ttcgggggct ttaacctggt cgaccgggcg catgtcgccc
     1141 gattcggggg tagtgttgta gaacgactgg ccgaccttat cgaggtggcg cgggtggtcg
     1201 accgtgcgaa tctgcagcgt gtcgagcagc accgggtttt cccattggtt gacgtctttg
     1261 ctcgtcatgt tgcgcgggtc ggtgccgaca gcctggttga tccagacttc gccgttcgac
     1321 ttgatgatgt agttgttgtg gtttacctgt accggtcctt tctcgcccat cagcggaaaa
     1381 ccgtcggggg taacgagcat gccgttgcgg tcgagtatga acgaaccgct gcgtgtcatg
     1441 cgcgggccgg cctgcgactg caccacaaaa aatccttcgc cgttgatggc caggtcctgg
     1501 tcgcgttcgg tacgcttgag cgcgccctgg tcaaagcggg tataaatttc attcacttcg
     1561 gcgcctgtgc cgagtttacc cacgagcggc gaaatatcga agccgcccat cggtacccag
     1621 cccacgccgt cgtcgcgcgt gcggtgaatc agcacctcgg ggttggccct gaagatggtg
     1681 acgtcttctt taaaggcggt tttgtcgacg ttggcgaggt tgttcgccac cgtatccatt
     1741 ttgacctgca tcgcggccat gcccgatgcg cctgtataga taccccgcag catggtttat
     1801 tatcgggggg agttttgaaa gtgctgagac ataatttggg ttttggccag ttatcgtgat
     1861 caaactgttc agaaatttcc ttgccggagt ggttttctgc aatgctgtgc agcccgtatt
     1921 atgtcgcatt aagggcgaat ggtctgccgg gatcggcagc actcggacaa ggctaaatat
     1981 tgacaaaagg atcagtttat ccaaaataga gcatgatcag gactctccgg atttctggcg
     2041 cctgatttcg tccacgtgca acccgcatct ttcaaggcgc gccccggaaa agattttgcc
     2101 ttgcaggtgg gcgtctatga atcggtgaca cgtgcagaag ttattgcact gcgcgccgat
     2161 cttcccccag gtaaccaggg cgctaaagtg agcgaccagc tggttacaga cgtaagccaa
     2221 cagatgcacg agaagcttga gcagcagcgc aagcagcgac tcaaagaaat cgaagcagaa
     2281 aagaagcgcg tcgcggaaat gcgcgcaaag aaccccgact acgattcagc cgccgcaccg
     2341 ggagatccgc aggcgaaagt aacactcaag cgcgcgatca cgaccggttg gccgacaaaa
     2401 agccgggaga tcgccttcct cggcggtttt gaacgcgtga gcggcggtgg gcaatcctca
     2461 tcggggacct ctttcctcgc attgcgcttt tttcctggtg ataaggcagc tgtgaggttg
     2521 ggcgtctttg gtggtgcttc gcttattcct ggtccaaaga ccagcgactg gggaggtgtt
     2581 ccacctgcgg ccggcgtctc gttcctcata gtccgcaatc ttgcgctgga agtcactgtc
     2641 gatgctgctt actactcctc tgtatccaga atgggttatg gtgtcggtta tggcataacg
     2701 ataccctttt agcaagccag catcgtgcag aactaattta gtagcgcgta taactaattt
     2761 ctgccaaacg ggcctgctca tgcagacaaa acaagacctg cgcagttctg aaactgagcc
     2821 caaagcacac tcgtggttct ctaaccctct cgactggtct catatcgacc ggctcatcct
     2881 gctggctggt atggtgtttt tggtgccgct cttcttcggc ggagttctgg cgctgacgtt
     2941 gctgcttgca cctgagtggc tgaatagccg tgtcgcgatc ccgatgtttg ccctgcacac
     3001 aggctattta acaataatac tgatgttcgt actcattgcc ctgaaaaaga gacgctccat
     3061 ttctacctgg ccgttatttg aagatgttat catctacagt tatgtggcgg tggtgttta
//
LOCUS       DAOQJF010000373         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_20757_length_3115_cov_3.881046, whole genome shotgun sequence.
ACCESSION   DAOQJF010000373 DAOQJF010000000
VERSION     DAOQJF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20757_length_3115_cov_3.881046"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLF85_15185"
     CDS             complement(<1..571)
                     /locus_tag="PLF85_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21063.1"
                     /translation="MLTLNRRRAVAAAALALLFVVPLPLTSQTKPAEPLSMQVAKRGD
                     AKNTHRIETSNFTVVFSEQGAQILEFRNRDNAYPRRDGANIVIPGTEIFLAPYLGATG
                     TAQYVGANLAAMMYAAFSFSKTENDDTVKIVATAPVVLLADKAKYDATFVKEFVFLKK
                     SPYFKFSLEIQTNAPVKLQIGAKLTKGLGA"
     gene            complement(640..1896)
                     /locus_tag="PLF85_15190"
     CDS             complement(640..1896)
                     /locus_tag="PLF85_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNE21064.1"
                     /translation="MNYSLSDLLDNFRLKSHRENYSAVIKGIEAGVVFRGTNLWVLIF
                     AIFIASLGLNVNSPAVIIGAMLVSPLMGPIMGMGLAMGINDVPLLRKSLQNFGFAAGV
                     SLITSTLFFLVSPINEAHSELLARTSPNIYDVLIAFFGGLAGILATASKLKGNVIPGV
                     AIATALMPPLCTAGYGIATWQPRFFAGAFYLFIINTVFIALATLITVRFLHFPYREKP
                     DSEQAKRAKRIITLVTIMTILPSIYLGYQIVLEARFVQQAARFIEHEASIPGDYLLQK
                     QVTASNRSIVLTFGGKPIDQTQIAAMRSRLEIYGLTGTHLEVRQGFATVAEKKEQVSA
                     EAQREASTRLSRRVLNEARTQFAGITGVTIEEGTEVVADEDSHLLWIAVIRSEKVLPA
                     AEQRRIREWLRVRLQVDAISVFFVPE"
     gene            complement(1931..>3115)
                     /locus_tag="PLF85_15195"
     CDS             complement(1931..>3115)
                     /locus_tag="PLF85_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HNE21065.1"
                     /translation="WAHNFPHGSLRQNLTEFLAETEIVFGLWAAFLFVMIIAEQRELK
                     AATDYIDGLNFTEAKFVLVIMVMAAAKPVLRAAETFINGTARLLPLPQGAAFYFVALG
                     LGPILGSFITEPAAMTLIALILRQRLYSRDVSPKFAYATLGLLLVNVSVGGVLTHFAA
                     PPVLMVAGKWNWGLSYMFTHFGWRGLLTTLSGAALMIAVFPQEMKKIAPAEERKGHIP
                     LWLTLMHMFLLAMVVVFAHHENIFFGVFMIFLGLTVATKEYQEPLKLREGLLVGFFLA
                     GLVTLGNLQAFWLKPLLASLDVNALYFGATGLTAITDNAALTYLGSLSTGLSEPMKQA
                     LVAGAVTGGGLTVIANAPNPAGVGILRQSNFFQQNGMSPLGLLAGALVPTGIAIVFFW
                     VF"
BASE COUNT          752 a          826 c          875 g          662 t
ORIGIN      
        1 ccgcgcccag acctttggtg agtttggctc cgatttgcag tttgaccggc gcattcgtct
       61 ggatttcgag cgaaaacttg aagtacggag attttttcag aaagacgaat tccttcacga
      121 aggttgcatc atatttcgcc ttgtcggcca gcagcactac cggtgcagtt gcgacgattt
      181 tgaccgtgtc gtcgttctct gtcttgctga agctgaaggc cgcgtacatc atggcggcga
      241 ggttcgctcc cacgtactgc gctgtacccg ttgcgccgag gtatggggcc agaaagattt
      301 ccgtgccagg aatgacaata tttgccccgt cacgccgtgg gtacgcgttg tcgcggttac
      361 ggaactccag tatctgtgct ccctgctcag agaagaccac ggtaaaattc gaagtttcga
      421 tgcggtgcgt atttttcgcg tcaccgcgct ttgccacctg cattgaaagc ggctcagcag
      481 gttttgtctg gctggtcagg ggcaggggaa cgacaaaaag cagtgcaagc gccgcagcag
      541 ctactgcgcg ccgccggttg agggtcagca tataaggtta gaatcaggtt gtgggacagc
      601 cggtaaataa aattgtcgct ccgctttacc gcagccgagt tattcaggga caaagaagac
      661 ggatatggcg tctacctgca ggcggacgcg cagccactcg cgtatacggc gttgttctgc
      721 ggcagggagc accttttcac tgcggatgac agcaatccag agcaggtggg agtcttcatc
      781 ggccacaact tcagtacctt cttcaatcgt gacgcccgtt ataccggcaa actgtgtgcg
      841 ggcttcgttt aatacgcggc gtgaaagccg ggtagatgcc tcccgctgcg cttcggcaga
      901 aacctgctct ttcttttctg cgaccgttgc gaagccctgt cttacttcga ggtgcgttcc
      961 cgtaagccca tagatttcaa ggcgagaccg cattgccgcg atctgtgtct ggtcaattgg
     1021 cttgcccccg aaagtcagca cgatcgagcg gttggatgcg gttacctgtt tctgcagaag
     1081 ataatcgccg ggaatcgaag cttcgtgctc aatgaacctt gcagcctgtt gcacgaaacg
     1141 agcctccagt acaatttggt agcccaggta tatactgggc aggattgtca tgatcgtgac
     1201 gagggttatg atacgctttg ctcgttttgc ctgttctgag tccggctttt cccggtacgg
     1261 aaaatgcagg aagcgtacag taatcaaagt tgccagcgca atgaagactg tgttaatgat
     1321 aaacagatag aaagcacccg caaagaaacg gggttgccac gtggcaatgc cgtagccagc
     1381 ggtacagagc ggtggcatca gcgctgttgc gattgctacc cccggaatga catttccctt
     1441 gagcttactg gcagttgcga gtatgcctgc caggccaccg aaaaaggcga tcagaacatc
     1501 atagatattg ggcgatgtcc gtgcgagcag ttcagaatgt gcctcgttaa ttggcgacac
     1561 caggaaaaac agcgttgagg taataagact cacgccggca gcaaagccaa agttctgcaa
     1621 tgacttgcgc agcaagggaa catcgttaat gcccatggcg aggcccatgc ccatgattgg
     1681 acccatcagc ggcgatacca gcatggcgcc gatgatcaca gcaggggagt tgacgttgag
     1741 cccgagcgaa gcaataaaaa tggcaaatat caggacccac agattggtgc cccggaaaac
     1801 cacgcctgct tcgatgcctt tgatcacagc ggaatagttt tcccggtgcg atttcaggcg
     1861 aaaattatcg agcaggtcgc tcaggctgta gttcatctaa actccccctt gagggccagg
     1921 cccccgtgat ttagaacacc cagaaaaaga cgatcgcaat tcccgttggt accagcgccc
     1981 cggcgagcag accaagcggc gacataccat tttgctgaaa aaaattgctt tggcgcagaa
     2041 tgccgacacc cgccgggttg ggagcattgg cgattacggt taagccacca ccggtgactg
     2101 cgcctgcaac cagcgcctgc ttcattggtt cactgagtcc ggttgaaagc gagccaagat
     2161 atgtcagtgc cgcattatcc gtaatcgcag tcaaacccgt ggcgccgaaa tagagcgcgt
     2221 tgacgtcgag cgacgcgagc aggggtttga gccagaatgc ctgcagatta cccagggtga
     2281 cgagtcccgc aaggaagaag cccaccagca gtccctcgcg cagcttgagc ggctcctggt
     2341 attctttggt tgcgaccgtc agacctaaga agatcataaa aacgccaaag aagatattct
     2401 catggtgagc gaagaccacg accattgcca gtaaaaacat gtgcatcagc gtcagccaca
     2461 ggggaatgtg ccccttgcgc tcttcggccg gtgcaatctt tttcatctct tgagggaaaa
     2521 cagcgatcat cagtgccgca cccgatagcg tcgtcagcag gccgcgccag ccgaaatgcg
     2581 tgaacatgta gctcagaccc caattccact tgcccgcaac catcagaaca ggcggagcgg
     2641 cgaagtgtgt cagcacaccg cccacggaaa cgttgacgag cagcaaaccc agcgtcgcat
     2701 aagcaaactt aggggagacg tcgcggctgt agaggcgctg gcgcagtatg agtgcgatga
     2761 gtgtcatggc tgcaggttcg gtgataaaag aacccagtat ggggccaagg cccagtgcga
     2821 cgaaatagaa ggcggcgccc tgcggcaacg gaagcagccg tgcagtgcca ttaatgaatg
     2881 tctctgcagc gcgcagcaca ggctttgcag ctgccatgac catgataacg agcacaaact
     2941 ttgcctcggt aaaattgagc ccgtcgatgt agtcggtcgc agccttgagc tcacgctgtt
     3001 cggcaataat catcacaaag aggaatgctg cccataggcc aaagacaatc tctgtctcag
     3061 caagaaattc tgtcaggttc tggcgtaatg agccatgagg aaaattatgc gccca
//
LOCUS       DAOQJF010000374         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_20880_length_3103_cov_4.963255, whole genome shotgun sequence.
ACCESSION   DAOQJF010000374 DAOQJF010000000
VERSION     DAOQJF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20880_length_3103_cov_4.963255"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..765
                     /locus_tag="PLF85_15200"
     CDS             322..765
                     /locus_tag="PLF85_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21066.1"
                     /translation="MKRSDLFTVASHKVCQPQGIRRIWEIFLASAADGVYEMTLKRVS
                     DRRTSRQNRYLWKLCEILAEAIGYGETDHAKELVLSDAMERLGFGSHVNFRGRVSFVR
                     DSTALKDVEKFGQIIDELHAIADFMNEDVEPERHIILPEKAEGLK"
     gene            968..1438
                     /locus_tag="PLF85_15205"
     CDS             968..1438
                     /locus_tag="PLF85_15205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNE21067.1"
                     /translation="MTAHKFRRPDSERHTHAELSKRISYNQKTGELERTRWRAKGKVG
                     NLKSTGYMRVKIDGIDYLYHHVVFCLCHKRWPVEVDHIDRNKLNNRIENLRECTRTEN
                     QQVLGKRLIRSDGKRFTSISHALRSIGAPMSSHSWIIRAIKKGGKAYGYRWRFG"
     gene            complement(1435..2298)
                     /locus_tag="PLF85_15210"
     CDS             complement(1435..2298)
                     /locus_tag="PLF85_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21068.1"
                     /translation="MIVLATTNDTIEAVLNASHTTNALQCFASYRDITTTAYTPGRQL
                     QTTNGATAVRIVDPPGASTQRVIDHLSVYNSDTAAKTITIRFDDGTNERVLFLCSLGV
                     GEKLEYNDGQGFQVFANNGAIKQSLNQGTNATQSGKVQVVLGSDQTNNNATLNTWQDI
                     TGLSFPVTGNGTNTYYFRFVIFAVTNATATGYRFGVSGPTNNILGYTVYMPTSATASA
                     INNVATYDSTTLQTSSAVGTANVNIITIEGYIRPTADGSVIGRFACELAGPTNSVIAK
                     HGSFVEYQQMV"
     gene            complement(2716..>3103)
                     /locus_tag="PLF85_15215"
     CDS             complement(2716..>3103)
                     /locus_tag="PLF85_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21069.1"
                     /translation="ALRYTVGNDGRSVTVATIKIKRGATFRFTVKAQSRAGKEYDLTG
                     YTGRCQVFPDQDGGAALFTLTSGSSGFDIPTPSNGICNLTFSAAQTANFVVGNSYYFD
                     VRLSNGTDVIYMDPIKISVQWTGTSA"
BASE COUNT          726 a          771 c          868 g          738 t
ORIGIN      
        1 tggccaactc caacagcatc gacgggtggc ccggagccag aaggggaaac aggacgaaaa
       61 ctggtaacga tggtcaggat atggcctgcc ccatcggcga gcgacaaccg agacagaggg
      121 gacattggtt ctccagcgat cctgcggaga cgcgagaagg ggaaacagat tatgctgagt
      181 cagagcgtat ctacagaatc tggggcattg aatcccgact gggtcgagtg gctcatgggg
      241 tggcccatcg ggtggacaga cttaaagcca ttggcaatgg gcaggttccg gcagtggctg
      301 cgcgcgcatg gagaattcta agtgaagcga tctgacctat ttacagtggc atctcacaaa
      361 gtctgccagc ctcaaggcat tcgccggata tgggagatat tccttgcatc cgctgctgac
      421 ggcgtttacg agatgacact aaagcgagtc tccgaccgcc ggacatccag acagaaccgc
      481 tacctgtgga agctgtgcga aatcctcgcc gaggccatag ggtacggcga gacagaccac
      541 gcaaaagagc ttgtcttgtc tgatgctatg gagcggcttg gttttggttc tcacgtcaat
      601 tttcgtggta gggtatcttt cgtccgagat tctaccgcgc tgaaagacgt ggagaaattc
      661 ggccagatca ttgacgagct gcacgccata gccgatttta tgaacgaaga tgtagagcca
      721 gaacggcaca taattttgcc tgaaaaggct gaggggttga aatgagaaaa ttcacgatac
      781 acatttacga cagtggggca gggttcaacc catattctga gagtatcacc gtcgggatag
      841 aagctggaga aacgtgggac agagagacca ttgatgcgtt tgctgaaaaa gtacgggatg
      901 cggctagaga ttgctttggc gttaatcagc cgcgtgcgca tattattatg catgatgagc
      961 acgtccaatg accgcccaca aattccgcag accagacagc gagcggcaca cacacgcaga
     1021 actgtccaaa cgcatcagct acaaccagaa aaccggcgag ctggagcgga caagatggcg
     1081 cgctaagggc aaggtcggta atctgaaatc caccggctat atgcgggtca aaatcgacgg
     1141 catagactac ctgtatcacc atgtagtttt ttgcctatgc cacaaacgct ggccggtaga
     1201 ggttgaccac atcgaccgca acaagctaaa caaccgtata gaaaacctga gagaatgcac
     1261 acgcaccgag aaccagcagg ttctaggcaa aaggttgatc cgctcagacg gcaagcggtt
     1321 taccagcatc agccatgcct tacggtcaat cggcgcgcca atgtcctcgc attcgtggat
     1381 tattcgagcc attaagaaag gcggcaaggc ttatggctac cggtggcggt ttggttagac
     1441 catctgctgg tattcgacaa aagagccatg tttcgcaatt acgctatttg tcggcccggc
     1501 aagctcgcag gcaaaacggc caatcactga accgtcagcg gttggccgga tgtacccttc
     1561 aatggtgatg atgtttacgt tagccgtgcc tactgctgat gaggtctgca aagtggtgct
     1621 gtcataggta gctacgttgt taatggctga agcagttgcg cttgttggca tgtagacagt
     1681 gtaacctagt atgttgttgg tagggccaga gacaccaaaa cggtatccag tggcggtagc
     1741 gttcgtcact gcaaagatca caaaacggaa gtagtatgta tttgtgccgt ttcccgtaac
     1801 ggggaatgac aatccggtga tgtcctgcca tgtgttcagt gtggcgttgt tgttggtttg
     1861 gtcgctgcca aggacgacct gaaccttgcc ggattgcgtg gcgtttgtcc cttggttcag
     1921 gctttgtttt atggcgccat tgttcgcgaa tacctgaaac ccttgcccgt cgttgtattc
     1981 cagtttttca cccacaccaa gagaacatag gaaaagcacg cgctcatttg tcccatcgtc
     2041 aaatctgatg gtgatggttt tagccgctgt gtcgctattg tagacagata gatggtcgat
     2101 gaccctttga gtagatgcgc ccggcgggtc tacgatccgc acagcggttg ccccgtttgt
     2161 ggtctgtagt tgccggccgg gtgtgtaggc tgtggttgtg atatcacgat aagacgcgaa
     2221 gcattgcagc gcgttggtag tgtgcgaggc gtttagtact gcctcgattg tgtcgtttgt
     2281 agttgctaag actatcatgc tcttaaacct cttgctaaaa cttgtgcttg tgtcagacct
     2341 gtgtcagcgt ctgcccattc tgtatcgtaa tcggtggcgc tggccttacg taaaacttgg
     2401 ttggtattgc caccgatagg cacacccaca ccggggtcac cttgcagacc ttgtgggccg
     2461 gttgcgcctt gcgggcctgt tgcgcccgtt gagcccggat caccctgcgg ccctgcttca
     2521 ccctgaagac cttgtgggcc ggttgcgcct tgcgggcctg ttgcgcccgt tgggcccgga
     2581 tcaccctgcg gccctgcttc accctgaaga ccttgcggtc cttgcgcacc ttgggggata
     2641 aggtgaaata tcattgcagg ctccggcgtt acagtaaacg ccgggcctga ttcaacgatt
     2701 aactgaaacg atacttcagg cacttgtccc cgtccattgt actgagattt ttataggatc
     2761 catgtagata acgtccgtac cgtttgacag ccgaacatca aagtaatagg aattgccgac
     2821 aacgaaattt gccgtttgcg cggcgctgaa tgtcaggttg caaatcccgt ttgatggtgt
     2881 agggatgtca aaacccgaac tcccgcttgt tagagtaaac aaagctgctc cgccatcttg
     2941 atcagggaac acctgacacc ggcctgtgta tcccgtgagg tcgtattctt tgccggccct
     3001 gctttgcgcc tttactgtaa atcgaaacgt agcgccacgt ttgatcttta tcgttgcaac
     3061 tgtcaccgat cgaccatcat tgcctacagt ataccgtaaa gcg
//
LOCUS       DAOQJF010000375         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_20892_length_3102_cov_3.800131, whole genome shotgun sequence.
ACCESSION   DAOQJF010000375 DAOQJF010000000
VERSION     DAOQJF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20892_length_3102_cov_3.800131"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLF85_15220"
     CDS             complement(<1..369)
                     /locus_tag="PLF85_15220"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE21070.1"
                     /translation="MSQFRFSFPTPITFGAGVRKQVPEHFKGQGISSIMIVTDEMLSK
                     LPFFTQYADELKAAGLKVDIFSGVKGNPVKSQVMAGVEAFKKSGAKAILGIGGGAALD
                     VAKAIALMVNHPGDIFDYEDE"
     gene            complement(403..1770)
                     /locus_tag="PLF85_15225"
     CDS             complement(403..1770)
                     /locus_tag="PLF85_15225"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNE21071.1"
                     /translation="MEDKADFKKILERLEHANVKKVKVAVADIDGVLRGKYLHFDKFA
                     AAADSGFGFCNVVLGWDSADVCYDNVKYTGWHSGYPDQLVQLDPATERNVPWDNNVPF
                     FLGHFVKSDLSPLDICPRQTLRRVIERAEAMGFTAKNGMEFEWFNFRETAQSLDEKGY
                     IRPEPITPGMFGYSLIRAGQERDYFNALMDELTAFGVPIEGLHTETGPGVYEAAILYS
                     GALESADRATLFKAGAKDIAARFGIMPSFMAKWNASLPGCSGHMHQSLWNANGENAFY
                     DAAEAHRMSSTFKSYLAGQLKLLPELLPFFAPTVNSYKRLVDGYWAPVKPTWGIDNRT
                     VSHRVIPGSEKSTRLEVRVSGSDVNPYLSVAASLAAGLYGIENKLKLEDAPVKGSAYT
                     ESSVPRLPHNLLDATRRLADSKVAREILGADLVEHFVATREWEWRQFQDAVTDWETRR
                     YFEII"
     gene            complement(1821..>3102)
                     /gene="eat"
                     /locus_tag="PLF85_15230"
     CDS             complement(1821..>3102)
                     /gene="eat"
                     /locus_tag="PLF85_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HNE21072.1"
                     /translation="YVISGMYFGWNLGLPQSGPYGMLVATGIITLMYVCFVLSYSELA
                     AAIPRAGGVFVYSQRALGAFAGFIAGLAQNIEFVFAPPAIAAAIGAYLNIFFPAIEPV
                     GFAIAAYVLFTALNIWGVKESAAFGVFITILAVVELLLFTGLTLPHFDSAAFSRNALP
                     SGWWGILPAIPYAIWFYLAIEGVANMAEEVKNPQRDLSLGFNLSMATLVVLAVLVFLG
                     AVGVAGWEAVVYGSTELTTSSPGKLVMSDKPLPLALMRITGPNHPMYHLLVSIGLLGL
                     IASFHGILLVAGRTTMELSRARYLHHRFARLHHTRQTPAEALVLNMLLGIAILLSGKT
                     AEMIVLAAFGALTIYVMALVSQLVLRQTQPGLERPFRTPLFPYVPITALVIAIFSLGA
                     MVYFNGTLFVIYVALLAAGSIYFYLVHLRRQRDL"
BASE COUNT          729 a          890 c          870 g          613 t
ORIGIN      
        1 ttcgtcttcg tagtcaaaga tatcaccagg gtggtttacc atcagtgcga tcgccttggc
       61 gacatcgagc gcggctccac caccgatacc cagaatcgct tttgctccac tctttttgaa
      121 ggcctcaaca ccggccataa cctgcgattt gacggggtta cctttcaccc cgctaaaaat
      181 atcaaccttg agtcccgctg ccttcagctc gtcggcgtac tgcgtaaaga acgggagctt
      241 gctgagcatc tcgtcggtca ctatcatgat cgagctgatc ccctgtccct taaagtgctc
      301 gggcacctgc ttgcgcactc cggcaccgaa ggtaatgggt gtaggaaaac tgaagcgaaa
      361 ctggctcatg gtgcggattt catccgcgcc gtgagccagc aattagataa tttcaaaata
      421 ccgcctagtc tcccagtcgg tgacggcatc ctggaactgc cgccactccc attcgcgggt
      481 ggcgacaaag tgttcgacca ggtcggcacc cagaatttca cgcgcgacct tcgagtcagc
      541 caggcgacgg gtcgcatcga gcaggttatg cggcagccgg ggcacactgc tttccgtata
      601 ggcagaacct ttgaccggag catcctcaag tttcagcttg ttctcgatgc cgtagaggcc
      661 ggccgcgagc gaagctgcca ccgaaagata cgggttcacg tccgagcccg aaacgcgtac
      721 ttcgaggcgg gtagattttt ccgaacccgg tatcacccgg tgcgaaaccg tacgattatc
      781 aatgccccag gtcggtttga ccggcgccca gtaaccatcg acgaggcgct tatacgaatt
      841 caccgtcggg gcgaagaatg gcagaagttc tggcaacagc ttcaactggc cggcaaggta
      901 gcttttgaag gtggagctca tgcggtgcgc ctcagcggca tcgtagaaag cattttcacc
      961 gttagcattc cagagtgact ggtgcatatg acctgagcag ccgggcagcg aagcattcca
     1021 tttggccata aaactcggca tgataccaaa gcgcgcggca atatcttttg ctcctgcctt
     1081 gaagagcgtc gcgcggtcag cactctcgag cgccccgctg tagagaatcg ccgcttcata
     1141 gacgccgggc ccggtttcgg tgtgtagtcc ctcgatgggc acgccgaatg ctgtaagttc
     1201 gtccatcagc gcgttgaagt agtcgcgttc ctgcccggcg cgaatgaggc tgtagccgaa
     1261 catgcctgga gtgatcggct cggggcggat ataccccttc tcatcgaggc tctgcgctgt
     1321 ttcacggaag ttgaaccact cgaactccat accgttcttc gccgtgaacc ccatggcttc
     1381 ggcccgctcg atcacacggc gcagggtctg gcgcgggcag atatcaagcg gcgaaagatc
     1441 gctcttcaca aaatgtccga gaaaaaacgg aacgttgtta tcccacggaa cgttgcgttc
     1501 ggtagccgga tcaagctgca ccagctggtc gggataccct gaatgccagc cggtatactt
     1561 cacgttgtcg tagcagacat cggcagaatc ccaaccgagt acgacattgc agaagccgaa
     1621 gcccgagtcg gcagctgctg cgaacttgtc aaagtgcagg tacttgccgc gcagcacgcc
     1681 gtcgatgtca gcgaccgcga cctttacttt tttcacattg gcgtgctcga gccgttcgag
     1741 aatttttttg aagtcggcct tgtcttccat ggagcaccgt ttttccagtc ggcctcgttg
     1801 tggcagtctt ttttctttaa ctacaaatca cgctgccggc gaaggtgtac caggtagaaa
     1861 tagatggagc cggctgccag caaggccaca tagatgacga agagtgtgcc gttaaaatag
     1921 accatggccc ccagcgagaa gatggcgatc accagtgctg taattggcac gtacggaaac
     1981 agcggcgttc tgaacggacg ctccagcccc ggctgcgtct gccggagtac cagctgcgaa
     2041 accagcgcca tgacgtagat cgtcagcgca ccaaaagccg cgaggacaat catttccgcc
     2101 gttttgccag agagtagaat cgcaataccc agcaacatgt tgagcaccag tgcctctgcc
     2161 ggtgtctgcc gcgtatggtg caggcgggcg aaacggtggt gtaaatagcg cgcacgagaa
     2221 agctccatcg tggtgcggcc agcaacaagc agtatgccat ggaacgaagc aatcagcccc
     2281 aaaaggccaa tcgataccag caggtggtac atcgggtggt tgggcccggt gatacgcatg
     2341 agtgcaagcg gcaagggttt gtccgacata acgagttttc ccggagaact tgtggtgagc
     2401 tccgtcgaac catacacaac agcctcccaa ccggctacgc cgactgcccc aagaaagacc
     2461 agcaccgcga gtaccaccag tgttgccatg ctgagattga agccgagcga aagatcgcgc
     2521 tgcggattct ttacctcttc ggccatgttt gccacacctt caatggccaa atagaaccag
     2581 attgcgtaag gtatcgccgg cagtataccc caccagcctg atggcagcgc gttgcggctg
     2641 aacgcggcgg agtcaaagtg tggcagtgtc aggcccgtaa aaagtagaag ctccaccacc
     2701 gcgagtatgg tgatgaaaac gccgaaagcc gcagattctt tcacgcccca gatattgagc
     2761 gcggtgaaaa gtacgtacgc agcgatcgca aaccccaccg gttcgatggc tggaaaaaaa
     2821 atattgaggt aagcgccgat tgccgcagcg attgcaggcg gagcaaaaac gaattcgata
     2881 ttctgcgcga ggcccgcaat aaaaccagcg aaagcgccga gagcccgctg cgaatagaca
     2941 aaaaccccgc ccgcgcgcgg gatagccgcc gcgagctcag aataactgag cacgaagcag
     3001 acgtacatca gcgtgatgat gccggtggcg accagcatgc cgtaagggcc gctctgcggc
     3061 agccccaggt tccagccgaa gtacatgcca gagatgacat ag
//
LOCUS       DAOQJF010000376         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_21197_length_3077_cov_4.761085, whole genome shotgun sequence.
ACCESSION   DAOQJF010000376 DAOQJF010000000
VERSION     DAOQJF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21197_length_3077_cov_4.761085"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLF85_15235"
     CDS             complement(<1..399)
                     /locus_tag="PLF85_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE21073.1"
                     /translation="MPSLRAEFPFFAANPGVTYLDSAATALKPQSVVDALVRYNTSES
                     VNIHRGVYKLSQRATDSVEAVRERVTAFTLARKGATSVFVRGTTEGINLLAHALTSPA
                     SQLSPFFSSYTSPKKPAILISESEHHANIVP"
     gene            complement(467..1042)
                     /locus_tag="PLF85_15240"
     CDS             complement(467..1042)
                     /locus_tag="PLF85_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21074.1"
                     /translation="MKRSLALLLLALFACKKSAPAPAPDTAGKLRTALTSFFTAESDN
                     QRRAVGLWVADRASLERLAQKRGLLSGLAGQDSTAIKDRLASMEMFFHIGANGSYRSM
                     TIVKDQVGLAFGRLKSLKLGDKDQLYDATIKGKTETTAKIRILRQGAAEKFIYEEGTD
                     TIEATRETRTTAELVGIYAPRITAAGPVPPG"
     gene            complement(1039..1506)
                     /locus_tag="PLF85_15245"
     CDS             complement(1039..1506)
                     /locus_tag="PLF85_15245"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE21075.1"
                     /translation="MKKTSHLAFVAYIVLFTATCSSSPKISYATATDELVDAAQRGDE
                     HRVSEILAAGTGIDRKNNNGYTALMGAAERGQLVMVQKLVAANADVNATNKFSWNALM
                     LAVVGGHAEVAQTLISAGANVRYKTKNNETALMLAERGNHPEISQMLRKAGAR"
     gene            complement(1512..2312)
                     /locus_tag="PLF85_15250"
     CDS             complement(1512..2312)
                     /locus_tag="PLF85_15250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE21076.1"
                     /translation="MPDLHGKVIIVSGAAGGIGQELVSRLSSAGATVVATDVDEVALR
                     KVGGMARVEAVVCDVRKSDDWEKVFASAARLGSIYALINAVGVLKPGYIDTVSAKDID
                     FHIDINVKGVMLGSAIAAREFKRQGTGHIINIASLAGVAPIPGIGLYSASKFAVRGFT
                     LALAAELADAGVYVTVVCPDAVKTPMLDLQKDYPEAALTFSGSSPLTAADVAGAIVAV
                     IGKNEAEITLPIWRGVMAKAASALPGLTPLLVDTLRRAGLQNQAKYEK"
     gene            complement(2305..>3077)
                     /locus_tag="PLF85_15255"
     CDS             complement(2305..>3077)
                     /locus_tag="PLF85_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE21077.1"
                     /translation="YTCIAPYMRGYSQKNTPPGLLGDKAATVQIADLAADAAAMVEAA
                     GFSDALLVGHDWGAITAYAAANLAPQKFHSLVTLSVPHLRIFLGNLWKNPRQFLQSWY
                     ILFFQMRLSIPERSLQKDNFTFIEELWRRWSPDLHSSNEALAHVKEIFSDAQLLHNAL
                     AYYRGMLTPQVSELPQWNRSRELSFADIQVPTLTLTGSHDGCIVPEMFDGMHTAIQGE
                     FTLRILPTAGHFLTLESDERIAAEISKFTENYAEVKHA"
BASE COUNT          648 a          832 c          878 g          719 t
ORIGIN      
        1 aggcacaata ttcgcatggt gctcgctctc gctgatgaga attgccggtt ttttcggaga
       61 ggtataagag ctgaaaaaag gcgatagctg cgaagcgggc gaggtcagtg catgggccag
      121 caggttgatg ccctcggtcg tgccccgtac aaaaaccgag gtcgcgcctt tacgggccag
      181 cgtgaaagca gtcacgcgtt cacgcaccgc ttcaacgctg tcggtcgcgc gctgcgagag
      241 cttatagaca ccgcggtgga tgttcaccga ctcggaagta ttgtagcgta cgagtgcatc
      301 gactacgctc tgcggcttta gcgcagtcgc tgcggagtcg aggtaggtga ctccggggtt
      361 tgcggcaaaa aaggggaact cggcgcgaag cgatggcata actacaacaa agataccaaa
      421 aaaaaatcac tttgtcgtga ttttcagctg tgcatgctgc tttattctag cccgggggaa
      481 cggggcccgc agctgttatg cgcggcgcat agatgccgac cagttcagcg gtagttctcg
      541 tttcccgcgt ggcttcaatg gtatcggtac cttcttcgta gataaatttt tctgcagctc
      601 cctggcgcag aatccggatc tttgctgtag tctcagtctt gcccttgatt gtcgcatcat
      661 acaactggtc tttgtcgccc agtttcaagg atttcagacg accgaaagct agtcctacct
      721 ggtccttcac gatggtcata ctacggtatg aaccattggc accgatatgg aaaaacatct
      781 ccatgctcgc aagccggtct tttatcgccg tgctatcctg tcctgccaga ccggagagta
      841 aacctcgctt ctgtgcaagg cgttcgagcg aagcgcggtc ggccacccag aggcccacag
      901 cgcggcgctg gttatcagac tcggctgtga aaaagctggt gagcgccgtg cgtagcttgc
      961 ctgccgtatc gggcgccggg gcaggggcag atttcttaca ggcaaagagt gccagcagca
     1021 gcaaagccag ggatcgtttc accgggctcc ggctttgcgc agcatttggc tgatttccgg
     1081 gtgattgccg cgttcggcaa gcataagggc cgtttcgtta tttttcgtct tgtagcgcac
     1141 attagcccct gccgaaatca gcgtctgagc gacttccgcg tgcccgccca cgactgcgag
     1201 catcagcgca ttccacgaaa acttatttgt ggcattgaca tcggcattgg cagcgacgag
     1261 tttctgtacc atgaccagct gcccgcgttc ggcagcgccc attagtgctg tgtaaccgtt
     1321 gttgttctta cgatcaattc cagtacctgc agccaggatt tccgagaccc ggtgttcgtc
     1381 cccgcgctgg gctgcgtcga ccagttcgtc ggtggctgtt gcgtagctaa ttttgggcga
     1441 tgatgagcaa gttgctgtaa agagaactat atacgcgaca aatgcgaggt ggctggtttt
     1501 tttcatggct ctcatttctc gtattttgcc tggttttgca gaccagcacg tcgcagggtg
     1561 tcgaccaaca ggggagtcag acccggtaat gcgcttgcag ccttagccat cacgccgcgc
     1621 caaataggca gggtgatttc tgcctcgttt ttgccaatta ccgcgacgat cgccccggca
     1681 acatctgctg cggtcagcgg cgatgagcct gaaaaggtca gcgctgcttc cgggtaatct
     1741 ttctgcaggt cgagcatcgg ggttttaaca gcatccgggc agacgacggt gacgtaaacg
     1801 cctgcatctg ccaattcagc agccagcgcc agtgtgaagc cgcgcaccgc aaattttgat
     1861 gcgctgtaga gtccaatgcc aggaataggg gcaacacccg ccagcgaagc aatgttgata
     1921 atatggccgg taccctggcg tttgaattcg cgcgcagcaa ttgccgatcc gagcatgaca
     1981 cctttaacgt tgatatcgat gtgaaaatcg atgtcttttg cgcttacggt gtcaatgtac
     2041 cctggcttaa gcactcccac agcattgatc agcgcataga tggaacccag gcgtgcagcc
     2101 gatgcgaaaa ctttttccca gtcatcgctc ttgcgcacat cacaaacgac cgcttcgaca
     2161 cgcgccatac cgccgacttt gcgcagcgct acctcgtcga catcggtcgc tacgacggtg
     2221 gctccggctg agctgagcct gctgacgagt tcctggccga taccgcccgc agcgccgctg
     2281 actataataa ctttgccgtg tagatcaggc atgttttacc tctgcgtaat tttccgtgaa
     2341 cttgctgatt tcggccgcga tgcgttcgtc gctttcgagc gtgagaaaat gccctgcggt
     2401 tggcagaata cgcagcgtga attcgccttg tatggctgtg tgcataccgt cgaacatttc
     2461 aggtacgata caaccatcat ggctgccggt gagcgtaagc gtgggcacct ggatgtccgc
     2521 aaaagaaagc tcacggctgc ggttccactg cggtaattca gaaacctgcg gtgtcagcat
     2581 gccgcggtaa tacgccagcg cgttgtgcag gagctgcgca tcagagaaaa tctctttcac
     2641 gtgcgccagg gcttcattgc ttgagtgcag gtcaggcgac caacgccgcc agagttcttc
     2701 aatgaaggtg aagttgtctt tctgcaggct gcgctcggga atactaaggc gcatctggaa
     2761 aaaaagtata taccagcttt gcagaaattg cctggggttc ttccacaggt tgcccagaaa
     2821 aatcctgagg tgaggtacac tcagtgtgac gagcgagtgg aacttttgcg gagcaaggtt
     2881 ggcagctgcg tatgcagtga tagctcccca gtcgtgcccc acgagcagtg cgtcggaaaa
     2941 tccggctgcc tcgaccatgg ctgcggcgtc tgcagcgagg tcggcaatct gcacagtggc
     3001 cgctttatcg cctaaaaggc cggggggcgt atttttctgg ctatagcccc gcatgtaggg
     3061 agcaatgcac gtgtacc
//
LOCUS       DAOQJF010000377         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_21199_length_3077_cov_4.221377, whole genome shotgun sequence.
ACCESSION   DAOQJF010000377 DAOQJF010000000
VERSION     DAOQJF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21199_length_3077_cov_4.221377"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLF85_15260"
     CDS             <1..552
                     /locus_tag="PLF85_15260"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNE21078.1"
                     /translation="PLVSRSAGRTAPAYYSLRAFQVVDVKMENRRKKEKTPPPLYDRA
                     VALAARRLRSAADLKKKLIAENYPEDEVDAAITRLGEVHLIDDNLVAQSISRRYADRG
                     NRFILQKMKDKGVADEQESAFESLADEYDRALAAGKKKLRTLEKFEQKIRGKKLYQHL
                     ALRGFAGSTVQRVVRALTRFDDD"
     gene            complement(577..951)
                     /locus_tag="PLF85_15265"
     CDS             complement(577..951)
                     /locus_tag="PLF85_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21079.1"
                     /translation="MLHLRIVLLSLLFLSTACREKSLVIDDAEKPRTLTIQLPEGTES
                     FELKVHGLLSGEARLHLVAPGNEEPLKTLYLVPVVVAGMPRGIAPAATEIGYTGRELL
                     LKYEPGRKVDGHIEITYRFNTR"
     gene            1030..2049
                     /locus_tag="PLF85_15270"
     CDS             1030..2049
                     /locus_tag="PLF85_15270"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNE21080.1"
                     /translation="MPQHARIALLGTGAIGASVARALYLNQVPFTILVRDKKRKADLI
                     TQGIRYTLKSTTHIQLNQGVEVLTLAEAQKNARSLKFDYMFFGMKTPHLQEAARTAKG
                     LLAKGGRLVLLQNGLPERELRNYKEENIISGIVGYNAQLMPDGSYFQSNPGHLILANA
                     HRPPQEFKAWLEPHLPLVISQNPIGFRWNKLAINSVINGLGAISGLALGPLFAKRTAR
                     RAAIGLIQEAAAVMRALQIEEGIMPGAISVYRFTKLPALVQHLVLFVLGRKYAKIRTS
                     MLQDVERGVKTEVDQIHGAIVESAKVAGFAVPLTQKVVQLMHALERGAMQPGSEVLWQ
                     LPLPA"
     gene            complement(2061..2276)
                     /locus_tag="PLF85_15275"
     CDS             complement(2061..2276)
                     /locus_tag="PLF85_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21081.1"
                     /translation="MKYVLQKTVAIALLTVFSSTAHTEAKSSGIKEATKTPHAKIIVR
                     PGPPVKPTQPAKPVGNMKSDWSGKEKR"
     gene            complement(2287..2562)
                     /locus_tag="PLF85_15280"
     CDS             complement(2287..2562)
                     /locus_tag="PLF85_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21082.1"
                     /translation="MKIVTMTCVLLMPAIFLPAVSWAQMKRLPKYTKSKKASQPNANV
                     DPGKNLPGINSEGFRRPGDAAGSASSNDKYTPTLDPGVSAPVSGSSK"
     gene            2668..>3077
                     /locus_tag="PLF85_15285"
     CDS             2668..>3077
                     /locus_tag="PLF85_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE21083.1"
                     /translation="MKVLIVGGSGVAGQCAIRAVRQFDPQSEIWASSSKDADVAGADH
                     TVHAVDMNKPDGLAPLLALTEQFDYLFHTPAFGSVGAPVAEATKPEVESALHFCLQPM
                     VQLTEKLRPKMTVAYTAYYFKPHLLAFYGALAYV"
BASE COUNT          672 a          830 c          882 g          693 t
ORIGIN      
        1 ccactggttt cgcgatctgc cggtcgcact gcgcctgcgt attattcgtt acgcgcgttt
       61 caagtggtgg atgtgaaaat ggagaaccgc cgcaagaaag aaaaaactcc cccgccgctc
      121 tatgaccggg cggttgcgct cgcagcgcgc cgcttgcgca gtgctgctga tctaaagaag
      181 aagctcatcg cagaaaatta ccccgaagac gaagtcgacg ccgcgattac gcggttggga
      241 gaagtgcacc tgatcgacga caatctcgtg gcgcagtcga tttcgcgccg ctacgccgac
      301 cgcggtaacc gcttcattct gcagaagatg aaagataagg gcgtcgcgga cgaacaggaa
      361 tcggcgttcg agtcactggc cgacgagtat gaccgcgcgc tcgcggcggg taagaagaag
      421 ctacgtacgc tcgaaaaatt tgaacagaag atacggggaa agaagctcta ccagcacctg
      481 gctttgcgcg gttttgcggg ctctactgtg cagagggttg tgcgcgcgct cacacgcttc
      541 gacgatgatt gaatagtctt tttcgaggtc agtcaatcat cgcgtattaa agcggtacgt
      601 aatctcgata tgaccgtcga cctttctgcc cggttcgtat ttcagcagca gttcacggcc
      661 ggtatacccg atctctgttg cggcaggcgc aatgccacgc ggcatacccg cgacaaccac
      721 tggtaccagg tagagcgtct ttagcggttc ttcattaccc ggcgcgacaa ggtgcagccg
      781 tgcctcgccc gaaagcaggc catgcacttt gagctcgaac gactcggtcc cttcgggcag
      841 ctgtatcgtg agcgtgcgcg gcttctcggc atcatcgatg acgagcgatt tttcgcggca
      901 cgccgtgctc aaaaatagca acgatagaag aacaatacga aggtgcagca ttggggcaat
      961 ctgccggcgc gggatgcccg cacaaatgtt ttgcttttca gggtagccgc gccggcttta
     1021 ggtgcggcca tgccgcaaca tgcaagaatc gcgttactgg gaaccggagc catcggcgcc
     1081 tcggttgccc gcgcgctcta cctcaaccag gtgccattca cgattctcgt gcgcgacaaa
     1141 aagcgcaagg ccgatctgat cacccagggc attcgttaca cgctgaagag cacgacgcat
     1201 atccagctca accagggggt cgaagtactc acattggccg aggcgcagaa aaatgcgcgc
     1261 agtctcaaat tcgactacat gttttttggc atgaaaactc ctcatttgca agaggctgcc
     1321 aggacggcga agggtctgct cgcaaagggg ggccgcttgg ttcttctgca gaatggtttg
     1381 cctgaacgag agctccgcaa ctataaagaa gaaaatatta tttcggggat tgttggttat
     1441 aacgcgcagc tgatgcctga tggcagctat tttcagtcga acccagggca tttgatcctg
     1501 gcgaacgcgc accgcccgcc gcaagagttc aaggcgtggc ttgaaccgca cctgccattg
     1561 gtgatttccc aaaacccgat cggctttcgc tggaacaagc tggcgattaa cagtgtgatc
     1621 aatgggctgg gagcgatatc aggtttggcg ctcggcccgc tctttgccaa acgcacggcg
     1681 cgccgcgcgg ccattggtct cattcaagag gctgctgccg ttatgcgcgc gctacagatt
     1741 gaagagggaa ttatgccggg cgcgatctct gtctaccggt ttacaaaatt acccgcacta
     1801 gtgcagcacc tggtgctgtt cgtgctcggc cgcaagtacg cgaaaattcg tacttctatg
     1861 ttgcaggatg tcgagcgcgg ggtgaagaca gaagtcgacc agattcacgg tgcgattgta
     1921 gagtcagcga aagtggcggg gtttgctgtg ccgctaacac aaaaggtcgt tcagcttatg
     1981 catgcactgg aacgtggagc catgcagccc gggtccgagg tgctttggca actaccgctg
     2041 ccagcctgaa tcacgtacgc tcagcgcttt tctttgcccg accagtctga tttcatgtta
     2101 ccgaccggtt ttgccggctg tgtgggcttg accggtgggc cggggcgcac gatgatcttc
     2161 gcgtgcgggg tcttcgtcgc ttcttttata ccgctgcttt tcgcctcggt atgcgcagtt
     2221 gatgaaaaaa ctgtgagcag cgctattgcg acagtctttt gtaacacgta cttcatatgc
     2281 caaccttcat ttgctggagc cgctcaccgg ggcactgacc ccagggtcca gagtgggggt
     2341 gtacttgtcg tttgaagatg ctgagccggc agcgtcgcca ggccgcctaa acccttcgct
     2401 gttaatgccg ggaagatttt tgcctggatc aacattagca tttggttgtg aagccttctt
     2461 gcttttggta tacttaggta gtcgtttcat ttgagcccat gaaacagcag gcaagaagat
     2521 agctggcatc agcaagacac aagtcatcgt tacgatcttc atgagcctga ctacggttaa
     2581 acccattatc ccgcaaagca aaaaaatgag cccgctcggc agcactttaa ttgcttggca
     2641 tggctgcggt cagctttgcc attgcacatg aaagtactca tcgtcggcgg cagcggggtg
     2701 gcggggcagt gtgccattcg cgcagtgcga caatttgacc cgcagtcaga gatctgggcg
     2761 agcagttcaa aagatgcaga cgttgcaggc gctgaccaca cggttcacgc ggtcgacatg
     2821 aataagcccg acggtcttgc gcccttgctc gcgctcacag aacaatttga ttatttgttc
     2881 cacacacccg cattcggttc agtgggagct ccggttgcag aggcgactaa gcccgaggtt
     2941 gagtcggcgt tgcacttctg cctgcagccc atggttcagc tgacagaaaa actccgcccg
     3001 aaaatgacgg tggcgtacac ggcttattat tttaagccgc acctgctggc gttttacggc
     3061 gcgctggcct atgttaa
//
LOCUS       DAOQJF010000378         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_21213_length_3076_cov_3.509765, whole genome shotgun sequence.
ACCESSION   DAOQJF010000378 DAOQJF010000000
VERSION     DAOQJF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21213_length_3076_cov_3.509765"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..457
                     /locus_tag="PLF85_15290"
     CDS             20..457
                     /locus_tag="PLF85_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNE21084.1"
                     /translation="MSRREALPVYYFTVEIKGIQQGYFRSCSGLKSESEVVTIQEGGV
                     NTTELKLMGHNKYSNIVLKQGFVDAQLWALRQGYVNDEPGTKMQRIDGLIVHYGPGGK
                     RLHKWRFRKAWICKWEGPEFDATKNEISVESIEIAHEELTYEG"
     gene            460..1296
                     /locus_tag="PLF85_15295"
     CDS             460..1296
                     /locus_tag="PLF85_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21085.1"
                     /translation="MSDSQRNSTAPDFQPLGTSASLRMLGSVMGRIQPGSSSRGAGAA
                     RSETGEIPGGAAEKSAAAARGAAEPQSVLDAGWTPSVARAGMQVQQRQEAAGPLWSSL
                     PKADLKPVVSAVEADLGTYLAAQRETSGRGRAGSPELALLAGQSDAASIRASSRGTTT
                     GSGSDVQQAAVDISTRLLDAVRSHAAAQAVASDSRISLGDLTLIAFADSKRQMAAATS
                     HAEHTPDPHPLHKAIDNSAHKKFMEDRKGFDNKLKKVAELAIESLEKSKRIAKERFGS
                     HG"
     gene            1289..1744
                     /locus_tag="PLF85_15300"
     CDS             1289..1744
                     /locus_tag="PLF85_15300"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21086.1"
                     /translation="MAELEKAELHDCDRGGKLTVLFNPKEFTVEKQVQWTQKEGAMTD
                     EPPEEFTKPTPANLTVTLHFDTYEQKSSVMEYTSKVEKLALIISKEKPRPPLCKFVWG
                     KFVFQGVVESVQQKFTMFLRDGTPVRCEATVKMKKASSAATGKQGGAGG"
     gene            1747..2850
                     /locus_tag="PLF85_15305"
     CDS             1747..2850
                     /locus_tag="PLF85_15305"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HNE21087.1"
                     /translation="MSESQSPKVSLPKVTVGGKEIDLSDISSLVVKKDLDQPDYVQIS
                     LSNLGDTSGGSKFSAILKPGTDLVVKIKVEGEGEQELFKGFVHGDDPTYDAHSPVSTD
                     LQGMNAMHKLTRGRKTRTFTNQTDQQIIQKVVSEAGLSVNFGREPPTLRHEHLHQNNM
                     TDLEFIRMRAARTGRVIFCEDKTLYYTKREKDQGPVAKLDYNTQGTGALENFKPKMSA
                     AAQVKKVVCHGWDPFQSTRESQKMECKAEGGASPLGGESGSSAFGDNPEVHVFDIPFR
                     SKEEGDLLAKSILEERQMNFINAEATGLGNSQIKPGKVIELQLGGKESRFNGKYYVVG
                     CEFSYSHSSSGMGEGSGMGGFKGKYKLQRDAGM"
BASE COUNT          761 a          776 c          924 g          615 t
ORIGIN      
        1 gcagagaggt atctaagaca tgtcacgccg cgaggcattg cctgtttatt acttcaccgt
       61 cgagatcaag gggattcaac agggctattt ccgatcatgc tccggcctta agtcggaatc
      121 ggaagtcgtc acaatccagg aaggtggagt caataccact gagctcaagc tcatggggca
      181 caataaatat tcaaacattg tgctcaagca gggttttgtt gatgcacagc tgtgggctct
      241 gcggcaagga tatgtgaatg atgaaccagg caccaagatg cagcgcatcg acggcctgat
      301 agttcattat ggtccaggcg gtaagcggct acataagtgg cgttttcgca aagcctggat
      361 ttgcaaatgg gaaggccctg aattcgacgc aaccaagaat gaaatctcgg ttgagagcat
      421 tgaaattgct cacgaagaat tgacctacga aggttaaaca tgtcggactc acagcgcaat
      481 tcaacggcac cggactttca gccgctgggc acctcggcct cgctgcggat gctcgggtcg
      541 gtgatgggtc ggattcagcc cggctcgtct tcgcgtgggg ccggggcggc tcggtcggaa
      601 accggtgaaa ttccgggtgg tgcggcggaa aaaagcgcgg cggcggcgcg tggagcagcc
      661 gagccgcagt cggtgctcga cgcgggttgg acaccgtctg tcgcccgcgc cggaatgcag
      721 gtgcagcagc gtcaggaagc agccggaccg ctgtggagca gtctgccgaa agcggatctg
      781 aagccggtgg tgtcggcggt cgaagccgat ttgggaacgt atctggccgc ccagcgagag
      841 acaagcggac gggggcgcgc cgggagtcca gagctggccc tcctggctgg tcagtcggat
      901 gcggcgtcca ttcgcgcgag ctcacgcggc acgacgacgg gctctgggtc ggacgttcag
      961 caggctgccg tcgatatatc gacgcggctt ctcgatgcgg tgcgatcgca tgcggcggcg
     1021 caggcggtgg ccagcgacag ccggatatcg ctcggcgatt tgacgcttat tgcatttgcg
     1081 gatagcaaac ggcaaatggc ggcagcgaca tcgcatgccg aacatacgcc agatccgcat
     1141 ccattgcaca aagcaatcga taactctgca cacaaaaagt ttatggagga ccgtaaaggc
     1201 ttcgataaca agctcaaaaa ggttgctgag ctagcgattg aaagcctcga aaagtccaag
     1261 cggattgcta aggaaaggtt cggatcacat ggctgaacta gagaaagcag aactccacga
     1321 ctgcgaccgt ggcggcaaat tgacagtact ctttaacccc aaagagttta cggtcgaaaa
     1381 gcaagtccag tggacccaga aagagggcgc catgacagat gagccccccg aggagttcac
     1441 caagccgacg cctgcgaact tgaccgtcac gctgcacttt gacacctatg agcaaaaaag
     1501 ctcggtgatg gagtatacat ccaaggtgga aaagctcgcg ctgatcatca gcaaagagaa
     1561 gccgcgtccg ccgttgtgca aattcgtatg gggtaaattc gtattccaag gtgtggtgga
     1621 atctgttcaa cagaaattca ccatgtttct tcgtgatggc acgcctgtgc gctgtgaagc
     1681 gactgtcaaa atgaagaaag caagttcagc cgctaccgga aaacaaggcg gcgctggagg
     1741 ctaatcatgt cggaatcgca gagtcccaag gtcagcctac cgaaggtgac tgtaggcggt
     1801 aaagagatcg acctcagcga catttcgagc ctggtggtca aaaaggacct tgaccagccc
     1861 gattatgttc aaatctctct ttcgaacctg ggtgatacaa gcggcgggag caaattctcg
     1921 gcgattctga agccaggaac tgacttggtc gtgaagatca aggtcgaggg tgagggcgaa
     1981 caggagctgt tcaagggctt tgtgcacggc gacgatccaa cgtatgacgc ccactctccg
     2041 gtgtcgacgg atctgcaagg catgaatgcc atgcacaagc tgacgcgcgg tcgcaagacg
     2101 cggacgttta ccaatcaaac tgatcagcaa atcattcaaa aagtcgtgag tgaggctggt
     2161 ttgtcggtaa actttggtcg cgagccgcca acgcttcggc acgagcactt gcaccagaac
     2221 aacatgactg atctcgagtt tatccggatg cgggctgctc gaaccggtcg tgtgattttc
     2281 tgtgaagaca aaacgctcta ttacaccaag cgtgaaaaag accaaggccc agttgctaag
     2341 ttggattaca acacgcaagg cactggcgca ttggaaaact tcaagccgaa aatgtcggct
     2401 gcagcgcagg tgaagaaagt cgtttgccac ggctgggacc cattccagtc gactcgtgaa
     2461 tcgcagaaaa tggaatgcaa agccgaaggt ggagcttcgc cacttggtgg tgaatccggc
     2521 agcagtgctt ttggtgataa tccagaagta cacgtcttcg acattccatt ccgcagcaaa
     2581 gaagaaggtg atttgctcgc gaaatcgatt ctcgaagaac gccagatgaa tttcatcaat
     2641 gcggaagcaa ccgggctggg caactcgcag atcaagcccg gcaaagtcat cgagcttcag
     2701 cttggtggaa aagaaagtcg ctttaacggc aaatattatg tcgttggctg cgaattttct
     2761 tattcacaca gcagctcggg tatgggcgaa ggctccggca tgggcggctt caaaggcaaa
     2821 tacaaacttc agcgcgacgc aggaatgtga tcctcgtcgg gctttggcaa cctcccgcgt
     2881 gtcttcgctt cggatttcca tgaaaaatcg cttccgagtg ggccgatgta ggtcggtccg
     2941 actgcgtggt gcccgtgcct ggtcggggcg cacctgcggg cttttggcca tcggacggtt
     3001 tgccggtccg cccaatccct tggtagggta agaggatggc tgatttgcaa cagcgtgggc
     3061 ccacccaagt ggcgcg
//
LOCUS       DAOQJF010000379         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_21248_length_3073_cov_4.083499, whole genome shotgun sequence.
ACCESSION   DAOQJF010000379 DAOQJF010000000
VERSION     DAOQJF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21248_length_3073_cov_4.083499"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..472)
                     /gene="fliS"
                     /locus_tag="PLF85_15310"
     CDS             complement(32..472)
                     /gene="fliS"
                     /locus_tag="PLF85_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNE21088.1"
                     /translation="MQGDLHLKEYQANSVGTADQKQLIVMLYEGVLRFVSAAESNMAS
                     FKTYDKANSNILRAQDIFTELMVSLNLEQGGEIAQNLFNLYAYCKTQLLEANVQKKVE
                     LLKPVKKITTELLAAWKQVEQNPTGPKPSAGKPADYKGGFKAEG"
     gene            complement(609..734)
                     /locus_tag="PLF85_15315"
     CDS             complement(609..734)
                     /locus_tag="PLF85_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21089.1"
                     /translation="MKILATVLLTALLAASAVSACDGKDGEKSKTKDKTKTEQSR"
     gene            884..1897
                     /locus_tag="PLF85_15320"
     CDS             884..1897
                     /locus_tag="PLF85_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21090.1"
                     /translation="MRNTITLALLLTGLVACKEAATAPKVDTKAVFFGSPEEELKPTI
                     WDTGDTTVTNEDRLEIYEPHLRGIGGGYIGVGSIQNFTLAAWAKSEYIWLMDFTRVVV
                     ATNKVHVAFLKRLATPQEHRAMWDKKKSAAAADKIIEEEFAGSPEIPFIKESYRKASP
                     YLMITFGFIDKLTKKRPYKFWLNDQEQYDYLKKLALENRIRPIKGNLNGDTTVLGIGE
                     AARKMGVPIRIMYFSNAEEYFRGYKEPFKKSFGTLPVDEKSLVVRTISFNKGKFRWAE
                     DSHLSTDRGFHYSLQPAQHFQEQLKLEKVDVRDFYTTAFIDPKEYGLSYLGPLAEKKR
                     QEK"
     gene            1897..2949
                     /locus_tag="PLF85_15325"
     CDS             1897..2949
                     /locus_tag="PLF85_15325"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNE21091.1"
                     /translation="MQAEAGTQRLSAQIARGILRGYKPSSKVLPKIAQLIKANKKFLL
                     TTHVGADADGLGSQVGLYYLLKALKKDVVILNNEKVPEYLLPLIPEKLVHYIGRHDLP
                     EEKAEKMMRDRFVFILDSSEPHRSEHVGEMFLRLKRPWATIDHHVLPGKKEYCVDPTY
                     AATAELVWDLYQYLKKPVSKQAALALYTGMVADSGNFRYPKTSLRSHLMGGHLLSFGI
                     HSDFVYRAIYESNPLDRLKLNARLYPKVELLPNKVVYVPVLQKDREGLELGDSGTEGI
                     VNHLLAHRGLKAAALFSETEHGELKGSLRSIGDVDVASVAKKFGGGGHKNAAGLKVRE
                     PFAEALKKVVAALSTL"
BASE COUNT          693 a          830 c          841 g          709 t
ORIGIN      
        1 aaatgagcaa gatatcttcc atcgaagacc gttaaccttc ggctttaaaa ccgcccttat
       61 agtcggcggg tttgccggct gagggtttgg ggccagtggg attttgttcg acctgtttcc
      121 acgctgcgag cagctcggtg gtaatctttt ttaccggttt gagcagctcg acttttttct
      181 gcacattggc ttcgagcagc tgcgtcttgc agtaggcgta gagattaaac aggttctggg
      241 caatttcgcc gccctgttct aggttgagcg aaaccatgag ttcggtgaaa atatcctgcg
      301 cgcgcagtat attgctattg gccttgtcgt acgtcttgaa cgacgccata ttcgattcgg
      361 ccgcagaaac aaagcgcagc acgccctcgt agagcatgac gatcagctgt ttctggtcgg
      421 cagtgccaac agagttggcc tggtattctt tgaggtgcag gtcgccttgc atgcttattc
      481 ttctcggtct cttcgaggtt aacttttcag caacctcata cagtgtcggc tttttttgtg
      541 cccctgttga gtaaaaacgg ccccccgcag tgcgcctttt tggcacgctt cgggaggccg
      601 ggactgactt agcgtgattg ctcagtcttg gtcttgtctt tagtcttgct tttctcgccg
      661 tctttgccgt cgcatgcgct tacagcggat gcagcgagca gggcggtgag gagcacagta
      721 gcgagtattt tcatgcttcc tgccttgtgg ttcactgtta tagctcccca caatcacaag
      781 gtagccttgg agatgctggc cgacgaaaaa aaggcgctga ggcggctttt tgccgtagag
      841 ccaaaaacgg tttgcgggct gaatctggct gctgttttgc ctgatgcgca atacgattac
      901 cctggccctg cttctgacgg gcctggtcgc ctgcaaagag gcggcgaccg cacccaaagt
      961 cgacactaag gctgtttttt tcggctcgcc agaagaagag ctcaaaccca ctatctggga
     1021 taccggcgac accaccgtca ccaatgaaga ccgcctcgag atctacgaac cgcacctgcg
     1081 cggcatcggc gggggctata tcggcgtggg ctcgatacag aatttcacgc tcgccgcgtg
     1141 ggccaaaagc gaatacatct ggctgatgga tttcacgcgc gtcgtggttg cgaccaacaa
     1201 ggtgcacgtg gcctttttga agcgcctcgc gacaccgcag gaacaccgcg cgatgtggga
     1261 taaaaagaaa tccgcggcgg cggccgacaa gattatcgaa gaggaatttg cgggcagccc
     1321 tgaaatcccc tttatcaaag agagctaccg caaggcgtcg ccttacctga tgatcacatt
     1381 tggcttcatc gacaagctca ccaagaagcg cccgtacaag ttctggctga acgaccagga
     1441 gcagtacgac tacctgaaga agctggcgct cgaaaaccgc attcgcccga tcaagggcaa
     1501 cctcaacggc gatacgacgg ttctgggcat cggcgaagca gcacgcaaaa tgggagtgcc
     1561 gattcgtatc atgtatttct ccaacgctga agagtatttt cgcggttaca aagagccgtt
     1621 caagaaatca ttcggcactc tgccggtcga cgaaaaatcg ctggtggtgc gcacgatttc
     1681 atttaacaag ggcaagttcc gctgggctga agattcgcac ctcagtaccg accgtggctt
     1741 ccactacagc ctgcagccgg cacagcattt ccaggagcag ctgaagctcg aaaaggtaga
     1801 cgtgcgcgac ttctacacga ctgcctttat cgacccgaaa gaatatggcc tgtcttacct
     1861 ggggccgctg gctgaaaaaa agcgccagga aaagtgatgc aggctgaagc gggtacgcag
     1921 cgcctgagtg cgcagatcgc caggggcata ctgcgcggtt ataaaccttc gtcgaaggta
     1981 ctgccgaaaa ttgcgcagct catcaaggcg aacaagaaat ttcttctgac cacccacgtc
     2041 ggtgcagacg ccgatggttt gggcagccag gttggcctct actacctgct caaggcgctg
     2101 aagaaagatg ttgttattct caacaacgag aaggtgcctg aatacctact tccgctgatt
     2161 cctgaaaaac tcgtgcatta tattggccgg cacgacctgc ccgaagaaaa agcagaaaag
     2221 atgatgcgcg accgctttgt cttcattctc gattcctcag agccgcaccg cagtgagcac
     2281 gtcggcgaaa tgttcctgcg cctgaagcgc ccgtgggcga cgattgacca ccacgttttg
     2341 ccaggcaaga aagaatactg cgtcgacccg acgtatgcag cgactgcaga actcgtgtgg
     2401 gatctctatc agtacctgaa aaagcctgtc agcaaacagg cggcgctcgc tctctatacg
     2461 ggcatggtgg ctgattcagg taactttcgt tatcccaaga catcgctgcg ctctcacctc
     2521 atgggagggc acctgctttc tttcggtatc cactcagact ttgtctaccg cgcgatttac
     2581 gaaagtaacc cgctcgaccg tttgaagctc aacgcacggc tctaccctaa ggtcgaactg
     2641 ctgccgaaca aggttgtcta tgtacctgta ttgcagaaag accgcgaagg tcttgagctc
     2701 ggcgacagcg gcacagaagg catcgtcaac cacctgctgg ctcaccgcgg gttgaaggct
     2761 gcggcgcttt tctcggaaac cgagcacggc gagctgaagg ggtcgctgcg ttcgatcggc
     2821 gacgtcgatg tcgcatcggt cgccaaaaag tttggcggcg gcggccacaa gaatgcggcg
     2881 ggtcttaaag tgcgtgaacc gtttgcagaa gcgttgaaga aagtcgtcgc ggcgctttca
     2941 acactgtaaa actcattcgc gtcagcgaat gagttttacg atcatctcga gtttttcggc
     3001 agcttccatc acttcctgca ccttgctctt gaagatcacg agcgcggtgt cggaaatctc
     3061 ttttttgact atc
//
LOCUS       DAOQJF010000380         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_21273_length_3071_cov_4.321286, whole genome shotgun sequence.
ACCESSION   DAOQJF010000380 DAOQJF010000000
VERSION     DAOQJF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21273_length_3071_cov_4.321286"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLF85_15330"
     CDS             complement(<1..1070)
                     /locus_tag="PLF85_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE21092.1"
                     /translation="MHKPDQNETTGAIRDQAPRSAEREHSVPIFMTSSFVFESAEQAR
                     ALFAEEQPGNIYTRFSNPNTDEFVRKMAVLEHLDDGFATASGMSAIFTSMAALLKSGD
                     HVVCARSVFGSTHQILTQIFPRWGIEFTYVDIADQANWQKAFKPNTRMLFLETPSNPS
                     LDIIDLEHVGKICKARGVLLNVDNCFCTPALQKPADFGADIVIHSATKYIDGQGRALG
                     GVILARKDLMKEIRFFARHTGPALSPFNAWIFSKSLETLDLRMERHSSNALALAQALQ
                     KNPEVEKVIYPFLPDHPGYALAKKQMKLGGGIVTMIVKGGIDRGRKFLDSLSMLSHTA
                     NLGDTRTIATHPASTTHSKLTDA"
     gene            complement(1063..1488)
                     /locus_tag="PLF85_15335"
     CDS             complement(1063..1488)
                     /locus_tag="PLF85_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE21093.1"
                     /translation="MSETRITLRRIGEPFHFEASNSAGNTVHIDAGAAIGGTGKGVRP
                     MEMLLMGLAGCSGIDVVSILRKQRQEIETMDVEVVGERGESQLANPFTKIVVRFALRG
                     PIEESHLKRAIELSMEKYCSVAKTLEMTATITWEYTLNA"
     gene            1582..1968
                     /gene="secG"
                     /locus_tag="PLF85_15340"
     CDS             1582..1968
                     /gene="secG"
                     /locus_tag="PLF85_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNE21094.1"
                     /translation="MADIFSTILTVMLIVVSILMIIIILLQSNRAAGGMFSSGAQTAF
                     GAGSADVLTKITGGFAAAFLLICFGLAALKSSTRATQAATLELGKESPAVVTPAPEKP
                     ADNKAPAAPAAKPAAPAAAAPAAAPK"
     gene            1974..2930
                     /gene="glpX"
                     /locus_tag="PLF85_15345"
     CDS             1974..2930
                     /gene="glpX"
                     /locus_tag="PLF85_15345"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNE21095.1"
                     /translation="MDRNLALEFVRVTEAAALAAARQMGKGDEKIADQAAVDAMRKAF
                     DSVLVSGKIVIGEGERDEAPMLYIGEEVGAGGTPVDIALDPLEGTTITAKGGYNATTV
                     IAVAEKGCFLHAPDTYMNKLAVGPAAKGALDITDPIPLTLKRLAKALDKYLEDVTVCI
                     LDRPRHNELIQQVREVGCRIKLIGDGDVSGAIATCMDDSPVDILLGTGGAPEGVLSAA
                     ALKCMGGDFQGQLKFRNDDERQRAIKMGISNPDKVFTMEELASGDSVLFCATGVTDGE
                     MLRGVRFNPKGAVTESIVMRARSRTIRWIKSHHHFDYKPKYL"
BASE COUNT          638 a          873 c          900 g          660 t
ORIGIN      
        1 gcatcggtca gcttcgagtg cgtggtcgat gcagggtggg tcgcaatggt gcgtgtgtcg
       61 ccgaggtttg ccgtgtgcga aagcatactg agtgaatcga gaaatttgcg accgcggtcg
      121 atgccgccct tcacaatcat ggtgacaata ccgccgccaa gcttcatctg ctttttcgcg
      181 agcgcgtaac cgggatggtc gggcaggaaa gggtaaatta ccttttcgac ttccggattc
      241 ttttgcagtg cctgcgccag cgcgagtgca ttacttgagt ggcgttccat gcgcaggtcg
      301 agcgtttcga ggctcttcga gaagatccag gcgttgaaag gcgaaagtgc cgggcccgta
      361 tgccgcgcga aaaagcggat ttctttcatg agatcttttc tcgcaagtat aactccccct
      421 aaggcacgcc cctggccgtc gatgtacttg gtcgccgagt gaatgacaat gtcagcgccg
      481 aagtctgcgg gtttctgtag cgcaggggtg cagaaacaat tgtcgacatt gagcagaaca
      541 ccgcgggctt tgcagatctt gccgacgtgt tcgaggtcaa tgatgtcgag cgacgggttc
      601 gaaggagttt cgagaaacag catgcgcgtg ttcggcttga aagctttttg ccagtttgcc
      661 tggtctgcga tatcgacgta ggtgaattca atgccccagc gcgggaaaat ctgcgtcaga
      721 atctggtgcg ttgagccaaa caccgagcgc gcgcagacaa cgtggtcgcc gctcttgagt
      781 aatgcggcca tgctggtgaa gattgccgac atacccgagg cagtggcgaa gccgtcgtcg
      841 aggtgctcga gtacagccat cttgcgcaca aactcgtcgg tattggggtt cgaaaagcgc
      901 gtgtagatat taccaggctg ttcttcagca aagagcgcgc gtgcctgttc tgcagattcg
      961 aagacaaagc tcgaagtcat aaaaattgga accgagtgct cgcgctcggc agagcggggg
     1021 gcctggtcgc gtatggcgcc cgttgtttcg ttttggtctg gcttatgcat tgagcgtgta
     1081 ctcccaggtg atggtagcag tcatttcgag cgtcttggcc acagaacagt atttctccat
     1141 cgaaagttcg atcgcgcgtt tcaggtggct ctcttcgatc ggcccgcgca gggcaaagcg
     1201 cacgacgatt ttggtgaagg ggttggcgag ctgtgattca ccgcgttcgc cgaccacctc
     1261 tacatccatc gtctcgattt cctgccgctg cttgcgcaga atactgacca cgtcaatacc
     1321 cgagcagccc gcgaggccca tcagcagcat ttccattggg cggacgcctt taccggtgcc
     1381 cccgatcgct gccccggcat cgatatgcac agtattgccg gcggagttgc tggcttcaaa
     1441 gtgaaagggt tcgcctatgc ggcggagcgt aatgcgcgtc tcggacatac gccgtggtgt
     1501 aggtctggca caggctgaaa accaatcccc agcccttctt atctgctcga caagctgtct
     1561 cgcgggacat ttttaccccc catggcagac attttctcga cgattctgac cgtgatgctg
     1621 atcgtcgtct caattctcat gattatcatc atcttgctgc agtccaaccg ggctgccggc
     1681 ggtatgttta gctcgggcgc gcagactgcg tttggcgccg gttcggccga tgttttaacc
     1741 aagattaccg gcggtttcgc tgcggctttc ctccttatat gcttcggtct ggcagcgctg
     1801 aaaagctcta cccgggcaac gcaggcagca actcttgagc tgggtaaaga atccccggca
     1861 gtagtcactc cggcccccga gaagcctgct gacaacaagg cccccgctgc acctgccgca
     1921 aaaccagcag ctcctgcagc agcagcaccc gctgcggccc ccaagtaata accatggacc
     1981 gtaacctagc cctcgaattt gttcgcgtta ccgaagccgc tgcgcttgct gcagcccggc
     2041 agatgggtaa gggcgatgaa aaaatcgccg accaggcggc cgtggacgca atgcgcaagg
     2101 catttgactc ggtactggtt tctggcaaga tcgtgatcgg cgaaggcgag cgcgacgaag
     2161 cgccgatgct ctacatcggt gaagaggtcg gcgccggcgg tacaccggtg gacatcgccc
     2221 ttgatccgct tgaaggtacg acgattaccg ctaagggtgg ctataacgcg actacagtta
     2281 tcgcggttgc cgaaaaaggc tgctttctgc atgcccccga tacttatatg aacaagctgg
     2341 ctgttggccc tgcagcgaag ggagcgctcg acattaccga tccaatcccc ctgactctca
     2401 agcggcttgc aaaggcactc gacaagtacc tcgaagacgt gactgtctgc attctcgacc
     2461 gcccgcgcca taacgaactg atccagcagg tgcgtgaagt cggctgccgc atcaaattga
     2521 tcggcgacgg cgacgtttcc ggcgctatcg ctacctgcat ggatgattcc cccgtagaca
     2581 ttctgctcgg tactggcggc gcacccgaag gcgtgctttc agcagcggcg ctcaagtgca
     2641 tgggcggcga tttccagggg cagctgaaat tccgcaacga cgacgaacgc cagcgcgcga
     2701 tcaagatggg catctcgaac cctgacaaag tattcaccat ggaagaactg gcgtcaggcg
     2761 acagcgtgct cttctgcgcc accggcgtga ccgacggcga aatgctgcgc ggtgtgcgct
     2821 tcaacccgaa gggtgctgtg actgaatcca tcgtgatgcg cgcgcgttcg cgtactatcc
     2881 gctggatcaa gtcgcaccac cactttgact ataagcccaa atacctgtga cacgccgcgc
     2941 ggttcgactg cgctcaccga gcggcactaa cacggtcact gagcgcagtc gaagtgacca
     3001 tggttgagca ccaaaacgcc cgcgaagaat tcaccaatat acgcgtgacg cgcgagggcc
     3061 ggtggtttac c
//
LOCUS       DAOQJF010000381         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_21469_length_3054_cov_5.280427, whole genome shotgun sequence.
ACCESSION   DAOQJF010000381 DAOQJF010000000
VERSION     DAOQJF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21469_length_3054_cov_5.280427"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1765
                     /locus_tag="PLF85_15350"
     CDS             122..1765
                     /locus_tag="PLF85_15350"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNE21096.1"
                     /translation="MEQIKNFLASRRATTILYVALFFSFSWLSYGAYLRFDLSSTGAM
                     RISGTTKNLLRSLPEKAVIELFVSNDLPDEAVLVSRKARDFIQEYVNSSRGRVKLTIL
                     DPDNDKSAAERARTLGIQQLDIAVRGSKKAQAQSIYFGLAISYGTKSETVNNLIGLYE
                     QQDLENQLTARIFKMVKPNERRIALLGGHGPFTLKKERSPNSISIFAEKVATFYGEIM
                     EINTSTADIPVEVSTLIVMQPGALSPMDKFRIDQFLMRGGNLIVAASGMEVSFGQQFM
                     ASPGNPDLTEFLKAYGIELSADMINEPKPNHFVPFVQPVNAMQMMKFPYPPWVVVTKD
                     GLSQENLATKGNAALTMPYTSSVKTNPAVIPAGEGKFKVDVLAKSTADSWAQTNFAFL
                     DPTKMEEMMAAPKQNAGTYNLAVMVQGKFSSQYAMQAPPKEAPKTWVKAAEKEASILV
                     LGSAFTFSNLTFILSEMTGLPLLEENNKMMFAAIDVMQGNQELVELRKKASPRIKPKL
                     VEDTGRKILTLLAFAIPLLGIAAAGVYRLTRRRNLNPGA"
     gene            1773..2720
                     /locus_tag="PLF85_15355"
     CDS             1773..2720
                     /locus_tag="PLF85_15355"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNE21097.1"
                     /translation="MPIQKKNTILAIATGAVFLLAFLVNDPFLIFEKSYEKSKPVISG
                     RAERVKKVTVSDGTQKRIFTRSTEGWSAELAGSPLGVQRADSQKIDTGLNNLFEARRY
                     QEVSSNKEKQADYEVRDTDFQLLLEGENGDKIAQVVLGKFSGAGNASFMRLADDTSVY
                     AVKGFLKGDWNQEFDLYRDRTVLRVARENVREIRASGKTSLTLKADDKGSFTLEPARA
                     TDKSRINTFLADLTELTGVKFHKDALPAPYGKISVLLSSNVTKEVEFFGPTKDQEFVA
                     RSSDMPSPVTIARSKVEALFPKLDDLLEKAPLPNLSSPK"
     gene            2754..>3054
                     /locus_tag="PLF85_15360"
     CDS             2754..>3054
                     /locus_tag="PLF85_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21098.1"
                     /translation="MKTTRWLRWKVLLLVVVVAATTTACRARALAEEGILRMEQGKQT
                     SALELFDRALRSNAQEPLALYGKGMLLADEQITQEIALAMLRQATQQSSLPEKYRV"
BASE COUNT          749 a          840 c          829 g          636 t
ORIGIN      
        1 accaggattc actttcggcg ggtctgctcg atacgcggga cctgctctac tacatttcat
       61 tttctgcaat ctttctctac ctgaataagg tgagcattga aaaattcaga cggggtcatt
      121 gatggaacag attaagaact ttctcgcttc gcgccgtgcc acaacgattc tgtacgtggc
      181 gcttttcttc agcttcagct ggctgagcta cggggcctac ctgcgttttg acctgtcatc
      241 caccggggca atgcgcattt cgggcacaac caagaacctg ctgcgcagcc tgcccgaaaa
      301 agcggtcatt gaactctttg tttcgaatga cctgcccgac gaagccgtgc tcgtttcgcg
      361 caaggcacgc gactttatac aggaatacgt caacagctcg cgcgggcggg tgaaactgac
      421 gatcctggat cctgacaacg acaagtctgc cgccgaacgc gcacgcacgc tgggcatcca
      481 gcagctcgat atcgccgtgc gcggttcgaa aaaagcgcag gcgcagagta tctacttcgg
      541 actcgcaatt tcgtatggca caaaatcgga gaccgtgaac aacctcatcg gcctgtacga
      601 acagcaggac ctcgagaacc agctcacggc gcgtatcttc aagatggtaa aacccaacga
      661 gcgccgcatt gcgcttttgg gcggtcatgg gccgtttacg ctgaagaaag aacgtagccc
      721 gaattcgatc tcgattttcg ccgaaaaagt agcaaccttc tacggtgaaa tcatggaaat
      781 caatacctct actgctgata tcccggtcga ggtttcgacg ctgatcgtga tgcagcctgg
      841 agcgctcagc ccgatggata aattccgcat cgaccagttt ttgatgcgcg gcggtaacct
      901 gatcgttgca gcatcgggca tggaagtcag ctttggccag caattcatgg cttcgccggg
      961 taaccccgac ctgactgaat tcctcaaagc atacggtatt gaactcagtg ccgacatgat
     1021 aaacgagccc aaacccaacc actttgtgcc gtttgtgcag ccggtgaacg ccatgcagat
     1081 gatgaaattt ccttatccgc cctgggttgt ggtgaccaaa gacggcttaa gccaggaaaa
     1141 cctcgccacg aaaggtaatg ccgcgctgac catgccgtac acttcgagtg tgaaaacaaa
     1201 tccggcggtt atacctgccg gcgaaggcaa gttcaaggta gatgttctcg cgaagtcgac
     1261 tgccgactca tgggcacaga caaacttcgc ctttctcgac ccgaccaaga tggaagagat
     1321 gatggctgct cccaagcaga atgctggtac gtataacctg gccgtgatgg tacagggcaa
     1381 gttcagtagc cagtatgcaa tgcaggcgcc gccgaaagaa gcgccgaaga cctgggtgaa
     1441 ggcggccgaa aaagaggcta gtattctggt gctcgggtcg gctttcacct tcagtaacct
     1501 gacctttatc ctgagcgaaa tgacagggtt gccgctgctt gaggagaaca acaagatgat
     1561 gtttgcagcc atcgacgtta tgcagggtaa ccaggagctc gtcgagctgc gcaagaaggc
     1621 cagtccgcgt atcaagccta agctggtcga agatacaggg cgtaaaattc tgaccctgct
     1681 ggcgttcgcg attccgctgc tcggcatcgc cgcggcgggt gtctaccgcc tgacacgccg
     1741 gcgcaacctg aatcctggag catgaggtaa ctatgccaat acaaaagaaa aatactattc
     1801 tggcaattgc caccggggca gttttcctgc tcgcctttct cgtcaacgat ccgttcctga
     1861 tctttgagaa gagctacgaa aaatccaagc cggtaatttc cggccgcgct gaacgcgtaa
     1921 aaaaggtaac agttagcgac gggacgcaga agcgtatctt tacgcgctcc accgaaggct
     1981 ggtctgctga acttgcgggc tcaccgctgg gcgtacagcg tgctgacagc cagaaaatcg
     2041 acacgggcct gaataacctg ttcgaagccc ggcgttacca ggaagtttct tcaaacaaag
     2101 aaaagcaggc agactacgaa gtacgcgaca cggatttcca gctgctgctt gagggcgaga
     2161 acggcgacaa gattgcacag gtcgtgctcg gtaaattttc cggtgcaggc aatgcgagtt
     2221 tcatgcgcct ggctgacgac acctcggtct acgcggttaa aggtttcctg aaaggcgact
     2281 ggaaccagga attcgacctt taccgcgacc gcacggtcct gcgggtcgcc cgcgagaacg
     2341 tacgcgaaat acgggcgagt ggcaaaactt ctctcacgct caaggctgat gataagggtt
     2401 cgtttacgct tgagcctgcg cgggcaacag acaaatcccg catcaatacg tttctggccg
     2461 acctgaccga gcttaccggg gttaaattcc acaaagatgc actgccagca ccctacggca
     2521 aaatcagcgt attgctctct tctaatgtga cgaaagaagt cgagtttttt ggtccgacca
     2581 aagaccagga gtttgtggcg cgcagcagcg atatgccgtc accggtcaca atcgcccgtt
     2641 ccaaagtcga agcgcttttc ccgaagctcg atgacctgct ggaaaaggcg ccactaccta
     2701 acctctcctc accaaaatag cgggatgcag cgtaatcttc cggggagaat cgtttgaaga
     2761 ccacgcggtg gctacggtgg aaggttctgc tgctcgtagt agtggtagct gcaacgacta
     2821 cggcctgccg tgcgcgtgcc ctcgccgaag aaggcatact gcgcatggag cagggaaaac
     2881 agacttcagc tctcgagctg tttgaccgtg cgctgcgcag caatgcacag gaaccccttg
     2941 cgctttacgg caagggtatg ctgctcgccg atgaacagat cacgcaggaa atagcgctgg
     3001 caatgctgcg gcaggcgacc caacagtctt cactgcccga aaaataccgc gtac
//
LOCUS       DAOQJF010000382         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_21769_length_3030_cov_4.719664, whole genome shotgun sequence.
ACCESSION   DAOQJF010000382 DAOQJF010000000
VERSION     DAOQJF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21769_length_3030_cov_4.719664"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..2362)
                     /locus_tag="PLF85_15365"
     CDS             complement(110..2362)
                     /locus_tag="PLF85_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HNE21099.1"
                     /translation="MRFHFPIIIIDKDFRAENTGGFGIRALAKALQDEGMDVTGVTGF
                     GDLSNFVQQQSRASGFVLSINDEDIAPTAEGQPSEILKQLEAFVAEIRLRNTDIPLFL
                     YSETRTSHHLPNSILKELHGFIHMYEDTPEFVARLIIREANAYLEKLPPPFFRALTQY
                     AHDGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENLLRADVCNAVEELGQLLDHRGPV
                     VESEVNAARIFQCDTLYFVTNGTSTSNKIVWHSAVAPGDIVVVDRNCHKSVLHAIVMT
                     GAVPVFLMPTRNHYGIIGPIPQSEFEWENILRKIRENPLAAKADGPPRILTITQSTYD
                     GVLYNVEEIKQMLDGKINTLHFDEAWLPHAAFHPFYRDMHAIGSDRPRTKKSMIFSTQ
                     STHKMLAGLSQASQILVQNSETEELDRDIFNEAFLMHTSTSPQYAIIASCDVAAAMME
                     APGGKALVEESIREAIDFRRAMRKVEADYESEWWFRVWGPTEIAAEGIGKREDWILTA
                     RDNWHGFGELADGFNMLDPIKGTVITPGLKLDGTFEDWGIPAMVVTHYLSEHGIVVEK
                     TGLYSFFIMFTIGITKGRWNTMVTELQQFKDAYDSNTALWRVMPRFTKQYPQYARIGV
                     KDLCQQMHEANRSSNIAQLTTHMYTSVMQPVLRPSEAYARMAHKRVKRVPIDDLEGKV
                     TAVLLTPYPPGIPLLIPGEMVNATIVSYLQATRRFNDQFPGFQTEIHGLVEEPNARGT
                     MDYFLDCVVD"
     gene            complement(2456..>3030)
                     /locus_tag="PLF85_15370"
     CDS             complement(2456..>3030)
                     /locus_tag="PLF85_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21100.1"
                     /translation="HSALTALTKSNTSPYAQVVALYDHEEIGSLSESGAETRLTEALL
                     SEIAVRYQLSTTDYQNLLRESVFLSADMAHGVHPNFADRHDTENRPRLGGGITLKINQ
                     NRRYATSAATHALFSDICRRHHIDHQVYTHRNDLPCGSTIGPTMSARLGMPTLDVGVA
                     MLGMHSARETCAWADVETYSRFMKFFLSGD"
BASE COUNT          605 a          843 c          880 g          702 t
ORIGIN      
        1 gacgaatttg cgtaggaatt cgcggcgatt aggccagctg agttcgccga aaaattgccc
       61 cagcgacctg ccgcggagcc ccctcagcgc tttgactgca agtagcgcgt cagtcgacga
      121 cgcagtccaa aaagtaatcc atcgttcccc gggcattggg ctcttctacc aggccgtgaa
      181 tctcggtttg gaatccggga aactggtcgt tgaaacggcg cgtggcctgc aggtaagaga
      241 caatcgtcgc attcaccatc tcaccgggaa tcagcagcgg aatacccggc gggtaggggg
      301 taagcagtac tgcggtgacc ttgccctcga ggtcatcgat cggcacgcgc ttcacgcgct
      361 tgtgtgccat acgcgcatag gcctcagaag gccgcaacac cggctgcatg accgaggtgt
      421 acatatgcgt tgtcagctgt gcaatattcg atgaccggtt tgcctcgtgc atctgctggc
      481 agaggtcttt gacaccgatg cgcgcgtact gtgggtactg cttcgtaaag cgcggcatca
      541 cccgccacag cgccgtgtta ctgtcgtatg catctttgaa ctgctgcagc tcggtcacca
      601 tggtgttcca gcggcctttc gtgatgccga tggtaaacat gataaagaag ctatagagcc
      661 ccgtcttttc gacgacgatg ccgtgctcgc tcaggtagtg cgtcaccacc atcgcgggaa
      721 ttccccagtc ttcaaaagtc ccgtcgagtt tcaggccggg agtgatgacc gtacccttga
      781 tggggtcgag catgttgaat ccgtcggcga gttcaccgaa accgtgccag ttgtcgcgcg
      841 cggtcagaat ccagtcttca cgtttgccta tgccctcggc tgcaatctcc gtgggtcccc
      901 aaaccctgaa ccaccattct gattcatagt cagcttcgac cttgcgcatc gcgcggcgaa
      961 agtcgatcgc ttcgcgtata gactcttcga cgagtgcttt gccgcccggg gcttccatca
     1021 ttgcggccgc cacatcgcag ctggcgatga tcgcgtactg cgggcttgtg ctcgtgtgca
     1081 tcagaaatgc ttcgttaaaa atatcgcggt cgagctcttc ggtctcggag ttctgcacga
     1141 gtatctggct tgcctgtgac aggccggcga gcattttgtg cgtcgactgc gtcgagaaga
     1201 tcatgctctt cttcgtgcgc gggcggtcag agccgatcgc gtgcatgtcg cggtaaaaag
     1261 ggtgaaaggc cgcgtgcggc agccaggcct cgtcgaagtg cagcgtgttg attttgccgt
     1321 cgagcatctg cttgatctct tcaacattat acagcacgcc gtcgtaggtg ctctgcgtga
     1381 tggtcaggat ccgcggcggg ccatcagcct ttgccgcaag ggggttctcc ctgatcttcc
     1441 ttaatatgtt ctcccattcg aattcagact gcggtattgg cccgatgatg ccgtagtggt
     1501 tgcgcgtcgg catcaggaag acgggtacag caccagtcat cacgatggcg tgcagcaccg
     1561 acttatggca gttgcggtcg acgaccacaa tgtcgcctgg ggcaaccgcc gaatgccaga
     1621 cgattttgtt cgaggtagag gtgccattcg tgacaaagta cagtgtgtcg cactggaaaa
     1681 tgcgcgccgc attcacttcg ctttcgacaa ccgggccgcg gtggtcgagt agttgcccca
     1741 gttcttcaac ggcgttgcag acgtcagccc gcagcaggtt ctctccgaaa aactggtgga
     1801 acatctggcc gaccgggctc ttgagaaatg ccacgccgcc cgaatgcccc gggcagtgcc
     1861 acgaataaga accgtcgtgc gcatactgcg ttaaggcccg aaaaaaaggt ggtggcagct
     1921 tttccaggta tgcattcgcc tcacgaatta tcaaccgggc gacaaattcg ggagtgtctt
     1981 catacatgtg tatgaagccg tgcagctctt tcagaatcga gttgggcagg tggtggcttg
     2041 tccgcgtttc gctgtagaga aagagcggaa tgtcagtatt gcgcagcctg atttctgcaa
     2101 caaatgcctc gagctgtttc agtatttcag atggctgtcc ctctgcggta ggagcgatgt
     2161 cttcgtcgtt gatcgagagt acaaaaccgc tggcgcggct ctgttgctgc acaaaatttg
     2221 acagatcacc aaaccccgtc acgcccgtga cgtccatgcc ttcgtcctgc agcgctttcg
     2281 ccagtgcacg tatgccaaag ccgccggtat tttcggccct gaaatcctta tcaataatga
     2341 tgatgggaaa atgaaaacgc atggaaatct ctgcccctat atcgcagctt ggcgtaaaga
     2401 gtattatgtc cttgatgtat ccgcagctgc gtagtgcgcc tgctgcgcat gtagctcagt
     2461 cgccagagag gaaaaatttc atgaaacgcg aatacgtttc gacatcggcc cacgcgcacg
     2521 tttcgcgcgc agaatgcatg ccgagcatcg cgacgccgac atcgagcgtg ggcatgccta
     2581 gccgcgcact cattgtcggg ccaatcgtgc taccgcacgg caggtcgttg cggtgcgtgt
     2641 acacctggtg gtcgatgtgg tgccggcggc aaatgtcgct gaacagtgca tgcgtcgccg
     2701 ccgaagttgc gtaacgccgg ttctggttga tcttgagagt aattcctcca ccgagacgcg
     2761 gccggttctc tgtatcatgg cggtcggcaa aattgggatg taccccgtgc gccatgtcgg
     2821 cactcagaaa gacactctct cgcagcaggt tttggtaatc tgtcgtgctt agctggtaac
     2881 gcaccgcgat ttctgacagc agcgcttcgg tgaggcgcgt ttcggcgccg ctttctgaca
     2941 gcgaaccgat ctcttcatgg tcgtagagcg ctaccacttg agcatagggg gaggtattgc
     3001 tttttgtcag ggcagtcagc gcgctgtggc
//
LOCUS       DAOQJF010000383         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_21865_length_3022_cov_5.169194, whole genome shotgun sequence.
ACCESSION   DAOQJF010000383 DAOQJF010000000
VERSION     DAOQJF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21865_length_3022_cov_5.169194"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..350
                     /locus_tag="PLF85_15375"
     CDS             171..350
                     /locus_tag="PLF85_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21101.1"
                     /translation="MHKQAIVSTIEQMPAEVEAEAVIEKILFLKNLEQGLADADEGKV
                     MSEDELRQRLGKLLS"
     gene            complement(639..1502)
                     /gene="purU"
                     /locus_tag="PLF85_15380"
     CDS             complement(639..1502)
                     /gene="purU"
                     /locus_tag="PLF85_15380"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNE21102.1"
                     /translation="MPENTTIHSLVLLVHCPDQPGIVARISNLIFSCGGNVTQLDQHT
                     TSPAEAQFFMRVRFDLAEANRARFDREGAVITQAIGGTWQVFDMTIPQRMVILVSKFD
                     HCLFDLLYRYSSGEFHVRIPLIISNHADLKPVADKYNIPFVHVPVTAQTKAESETRIL
                     EMVRGSSDLLVLARYMQILTPAFIESYSGEIINIHHSFLPSFKGAYPYERAYERGVKL
                     IGATAHYVTADLDEGPIIEQMVTRVSHRDTAEDMRLKGKNLEKLALASAVRCSLERRI
                     IRYKNRTVVFS"
     gene            1592..2824
                     /locus_tag="PLF85_15385"
     CDS             1592..2824
                     /locus_tag="PLF85_15385"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNE21103.1"
                     /translation="MNRRSLQFLALIVLFPLVFSTGAFAAKRKVAPKKVSTGLDELIK
                     NPAVLKDKRIALVVNHSAIDRKGRHIIDLLHPKFKVMKIFAPEHGVRGNFDEHVSGEK
                     DDKTGLPIISLYQKNKKAPTIEELADVDLLIFDIQEIGLRYYTYATTMVLAMKAAKAA
                     GKKMLILDRPNMAAPLGVYGPALESKFHGGFAGYYPIPIAHAMTMGELATFYNTHFSI
                     GADIEVVKMKNYSHNMFYDETGLPWRNPSPSITGMQSVLGYHLAGALEALNISVGRGT
                     SNPFQLYGAPFIEVKKIVKALNKARLPGVKFYAAKFKPTRSAYAKELCRGFKMVVTNR
                     RKVDPMRTLVTIARELYQQLPEKSREKNWQRIANGVGDTEFIAQIAKGERVEKLVQLT
                     QKDVDSFKQERKKYLMYP"
     gene            complement(2787..>3022)
                     /locus_tag="PLF85_15390"
     CDS             complement(2787..>3022)
                     /locus_tag="PLF85_15390"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNE21104.1"
                     /translation="LASYSDVALAAGNDKAVRAAASAIAQNPVAYLIPCHRVIRKTGA
                     FSEYRWGAVRKRMLIAHEAAKSTDTSSTSSALA"
BASE COUNT          702 a          734 c          895 g          691 t
ORIGIN      
        1 ccatccgccg cgaactgatg agcacgcgct gggccgtgat tgtattcttt actctgacag
       61 ggctggcgta tctggcgggg ctagttgtct ggcaggtggg cagtcggatt tcctgaccag
      121 agtattccta aaactgttga cacagcgatt tgtggcagcc agaggcatct atgcacaagc
      181 aggcgatcgt atcgaccatt gagcagatgc ccgccgaggt tgaggccgaa gcggttatag
      241 aaaagatact atttctgaaa aaccttgaac agggtcttgc cgatgctgac gagggcaagg
      301 tcatgtcaga agatgagttg cgccagcgtc tcggcaaatt gctgagctga gattcacagc
      361 aaagttcgcc gacgatctcg aagacatagc gcgttatatc aaacgggatt ctgtcattct
      421 ggcaagtgag tttgttctgc gtttgctcag ccagcttgaa gtactgaaag ctaatccgca
      481 gataggcaga atggtgccag agaaacagga tccagtggtg cgcgaaatct ttttcggcaa
      541 ttatcggatt gtttatcgtc tgcgcagtga cattgtcgaa ctaatcatgg ttcgccatgc
      601 tgcaagacta agatagcctt aatggcagtt ataactcgct aagaaaatac caccgtccgg
      661 tttttgtagc ggatgatacg gcgctcgagt gagcagcgca cggctgacgc gagcgccagc
      721 ttctccaggt ttttgccctt gaggcgcata tcttctgccg tgtcgcggtg tgagacgcgt
      781 gtcaccattt gctcgatgat cgggccttcg tcgagatcgg ccgtgacgta gtgcgcggtt
      841 gcaccgatca gtttgacccc gcgttcgtac gcgcgctcgt aaggatacgc acccttgaac
      901 gagggcaaaa agctatggtg gatgttgatg atttctccgc tgtaagattc aatgaaggcg
      961 ggtgtgagaa tctgcatgta gcgtgccagc accagcaggt ctgaactccc ccgcaccatt
     1021 tcaagaatgc gtgtctctga ttcggctttc gtctgtgctg tcacgggtac gtggacgaag
     1081 ggtatgttgt atttgtcagc aacgggtttg aggtctgcgt ggttcgaaat gatcagtgga
     1141 atccggacgt gaaactcgcc cgacgagtag cggtagagca ggtcgaacag gcagtggtcg
     1201 aatttggata ccaggatcac catgcgttgg ggaatggtca tgtcgaagac ctgccaggtg
     1261 ccgccgattg cctgagttat cactgcacct tcgcggtcga agcgtgcccg gttcgcttcg
     1321 gcgagatcga agcgaacgcg catgaaaaac tgcgcttctg cgggcgaggt ggtgtgctgg
     1381 tcaagttggg tgacgttgcc accgcagctg aagatcaggt ttgagatacg cgcaacgatg
     1441 ccgggctggt cggggcagtg gacgaggagt acaagcgaat ggattgtggt attttccggc
     1501 acgcagggta aaaaacacat ttttgacgtg gggtaaatgc aattcagcgc ttggacttta
     1561 cagccaccgc agtttgccga taatgatatt aatgaatcgc cgttctctgc aattcctggc
     1621 cctgatagtt ctgtttcccc tggtcttttc gaccggggct tttgccgcga agcggaaggt
     1681 tgcgccgaaa aaggttagca cgggccttga tgagctgatc aagaaccccg ctgtattaaa
     1741 agataagcgt atagcgctcg tggtgaacca tagcgctatc gatcgcaaag gccgtcacat
     1801 catcgacctg ttgcatccca agttcaaggt gatgaagatc tttgcgcccg agcacggcgt
     1861 gcgcggtaat tttgacgagc atgtctcggg cgaaaaggat gataagacgg ggctgccgat
     1921 tatcagcctc taccagaaga acaagaaagc tccgactatt gaagaacttg ccgatgtcga
     1981 tctattgatc ttcgacatcc aggaaatcgg gctacgttat tacacctatg caacgacgat
     2041 ggtactggcg atgaaggccg ccaaggcagc tggcaagaag atgctgattc tcgatcgccc
     2101 caacatggcg gcgccgcttg gcgtttacgg gccggctctc gagagtaagt tccacggcgg
     2161 ttttgcgggt tactatccga ttccgattgc acacgcgatg accatggggg agctagcaac
     2221 gttctacaac acgcacttca gcataggcgc tgacatcgaa gtggttaaga tgaaaaacta
     2281 cagccacaat atgttctatg acgagacggg tttgccgtgg cgtaacccaa gcccgagtat
     2341 cactggcatg cagtcggtac ttggttacca tcttgccggc gcgttagagg cgctgaacat
     2401 cagtgttggc cgcggcacca gtaatccatt ccagctctac ggagcacctt tcatcgaagt
     2461 gaagaagatt gtgaaggccc tgaacaaggc gcgcctgccc ggggtgaagt tttacgcagc
     2521 aaaatttaaa ccgacgcgtt ctgcctacgc gaaagaactc tgccgcggtt tcaagatggt
     2581 cgtgacgaac cggcgcaagg ttgatcccat gcgcacactg gtgaccattg cgcgcgagct
     2641 ctaccagcag ctgcctgaga aatcacgcga gaagaactgg cagcgtattg cgaacggtgt
     2701 aggtgacacg gagttcatcg cgcagatcgc taagggtgag cgcgtcgaga agctggtgca
     2761 gctgacgcag aaagatgtgg attcatttaa gcaagagcgg aagaagtact tgatgtatcc
     2821 gtagattttg ccgcttcgtg cgcgatgagc atgcgcttgc gcaccgcgcc ccagcggtat
     2881 tcactgaacg cgccggtttt gcgtatcacg cggtggcagg gaatcaagta cgctacgggg
     2941 ttctgtgcaa tggctgaagc agcagcgcgg acggctttgt cattacccgc ggccagggca
     3001 acgtcactgt aagatgcgag ac
//
LOCUS       DAOQJF010000384         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_22318_length_2985_cov_5.618430, whole genome shotgun sequence.
ACCESSION   DAOQJF010000384 DAOQJF010000000
VERSION     DAOQJF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22318_length_2985_cov_5.618430"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLF85_15395"
     CDS             <1..410
                     /locus_tag="PLF85_15395"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNE21105.1"
                     /translation="TSIENRQECCRIRKVEPLHRALKGAQLWITGIRRTHSAERGHLP
                     QAELDAANNVVKYHPLLEWDDAQLKAFIDQHNVPYNRLQDKGFLSIGCEPCTRAVKPG
                     ETMRSGRWWWEDPDKKECGLHVHAPAAVQGGSH"
     gene            410..1312
                     /gene="cysD"
                     /locus_tag="PLF85_15400"
     CDS             410..1312
                     /gene="cysD"
                     /locus_tag="PLF85_15400"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNE21106.1"
                     /translation="MTNVRTHLDYLESEAVMILREVAGQFERPALLFSGGKDSIALVK
                     LAEKAFRPAKFPFPLVHVDTGHNFDEVIQYRDDLVKKLGERLIVASVEDSIRRGTAAE
                     EKGKFPSRNAIQAVTLLEAIAEHRFDCCIGGARRDEEKARAKERIFSVRDEFGGWDPK
                     NQRPELWHVYNGNIHHGENVRVFPLSNWTELDVWLYLKRENVELPSIYFSHEREIIPF
                     MGQIFPVSKFIAIDAADKLEKRRVRFRTVGDMTCTAAMESEAADVDAIIHEIMTTRSA
                     ERGARLDDKRSEAAMEERKKGGYF"
     gene            1314..2561
                     /locus_tag="PLF85_15405"
     CDS             1314..2561
                     /locus_tag="PLF85_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE21107.1"
                     /translation="MDLLRVFTCGSVDDGKSTLIGRLLYDAKSIFQEHLDHIESKDRV
                     GDGELNLALLTDGLKAEREQGITIDVAYKYFSTPKRKFIIADTPGHVQYTRNMVTGAS
                     LADTAIILIDARQGILEQTRRHSFISHLLRVPHLVVCVNKMDLVGFSAERFAEIETEF
                     KKMSAELSFKQTTLIPISALKGDNIVNPSAAMPWYKGKPLLTTLEESDVHRKNAEGTR
                     FTVQYVLRPQSTDLHDFRGFAGVLRSGTLRKGDKIRVEPSGEEASITALHYSGKPADE
                     IYADDSAVVELDRDIDISRGDIFTHTGEQTPKGSLYKVYLTHLDTTPLKLNYAYYLQT
                     GTRKVKAMVKSIESKLDIHSLKFAAADGSVGLNEIAEVTLKTAQPVVFDTYAQNWTTG
                     AGVLVDAQTHMTACAVMNAGSAE"
     gene            2558..>2985
                     /locus_tag="PLF85_15410"
     CDS             2558..>2985
                     /locus_tag="PLF85_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE21108.1"
                     /translation="MKGTVTIAGAGPGELEHLTVGALRAIHAADVILYDALIGDSIIR
                     EFPSKAKTIFVGKRCGNHAYTQTMIIAAMIEHAMAGRNVLRLKGGDPAIFAHLSAELE
                     ALKNLGIETRILPGVTAMLTAAADLGRPLTTRGSNRHIWIT"
BASE COUNT          727 a          844 c          827 g          587 t
ORIGIN      
        1 ataccagcat cgaaaaccgc caggaatgtt gccgcatccg caaggtagag ccgctgcacc
       61 gtgcgctcaa gggcgcgcag ctgtggatta ccggcatacg caggacgcac tcagccgaac
      121 gcggtcacct gccccaggct gagctcgacg cagcaaacaa cgtggtaaaa taccatccgc
      181 tgctcgaatg ggacgacgcg cagctgaaag cttttattga tcagcacaat gttccctaca
      241 accgcctgca ggataaagga ttcctttcga tcggctgcga accgtgcacg cgcgcggtga
      301 aacccggcga aactatgcgg tccgggcgct ggtggtggga agacccggat aaaaaagaat
      361 gcggcctgca tgtgcatgcg ccggcagcag tacagggggg ctcccactga tgacaaatgt
      421 gagaacgcat ctcgactacc tcgaatcaga ggcagtcatg attctgcgcg aggtggcagg
      481 acaattcgag cgccctgcac tacttttttc cggcggtaaa gattcgatcg ctctggttaa
      541 gctcgctgag aaagcatttc gccctgcgaa atttcctttt cctctggtgc acgtcgatac
      601 cgggcacaat ttcgacgaag ttatccaata ccgcgacgac ctggtgaaga agctgggcga
      661 acggctgatt gtggctagcg tcgaggacag catacgccgc ggtactgctg ccgaagaaaa
      721 aggcaagttc cccagccgta acgcgattca ggcggtaacg ctgctcgaag cgattgccga
      781 acaccgcttt gactgctgca taggtggagc gcggcgcgac gaagaaaaag cgcgcgctaa
      841 agagcgtatc ttctcggtgc gtgacgagtt tggcggctgg gaccccaaga accagcggcc
      901 cgagctctgg cacgtctaca acggcaatat ccaccacggc gaaaacgtca gggttttccc
      961 actctctaac tggactgagc tggatgtctg gctctacctg aagcgcgaaa acgtcgaact
     1021 gccttcgatc tatttttcgc atgagcgcga aattatacct tttatgggac agatttttcc
     1081 tgtatcaaaa ttcattgcca tcgatgcagc ggacaagctc gaaaagcgcc gcgtccgttt
     1141 ccgtactgtc ggcgacatga cctgcaccgc agcgatggaa tcagaagctg ccgacgtcga
     1201 tgccatcatc cacgaaatta tgaccacgcg cagcgctgaa cgcggggctc gcctcgacga
     1261 caaacgctcc gaggcagcaa tggaagagcg caaaaaaggc gggtatttct agtatggacc
     1321 tgttacgagt atttacctgc ggttcggttg atgacggcaa gtcgacgctg atcgggcggc
     1381 tgctctatga tgcgaaatct atcttccagg agcacttgga ccatattgag tcaaaagacc
     1441 gcgtcggcga cggcgagctc aacctcgccc tgctgactga tggcctcaag gcagaacgcg
     1501 agcaaggtat cacgatcgac gtggcctaca agtatttctc tactcccaaa cgcaagttca
     1561 tcatcgccga tacaccgggg catgtacagt acacgcgcaa catggtgacg ggggcctcac
     1621 tcgccgatac ggcaattatt ctcatcgatg caagacaggg aattctcgag cagacgcgcc
     1681 ggcacagctt tatctcgcac ctgctgcgcg tgccgcacct ggtagtctgt gtcaacaaga
     1741 tggatcttgt gggctttagc gcggaacgtt ttgccgagat tgagactgaa tttaaaaaga
     1801 tgtctgccga gctttctttc aagcagacaa cgctgatccc tatctcggcg ctcaagggcg
     1861 acaatattgt aaacccatcg gcagctatgc cgtggtacaa gggcaaaccc ctgttgacca
     1921 ctctcgaaga gagcgacgtg caccgtaaga atgctgaagg tacgagattc acggtacagt
     1981 acgtcctgcg accgcaatcc accgacctgc acgacttccg cggctttgcc ggcgtcctgc
     2041 gctcgggtac gctgcgcaaa ggcgacaaaa tccgcgtgga gccctcgggc gaagaagcct
     2101 ccattacggc attacactat tcgggcaaac cagcggacga aatttacgcc gacgattcgg
     2161 cggtcgttga gctcgaccgg gacattgaca tcagccgcgg cgatattttc acgcataccg
     2221 gagagcagac gcccaagggt tcactttaca aggtttacct gacacaccta gacacgactc
     2281 cactcaaact gaactacgcc tactacctgc agacaggcac ccgcaaggta aaggcaatgg
     2341 tgaaatccat cgaaagcaaa ctcgatatcc attcgctgaa gtttgctgcc gcggatggca
     2401 gcgtgggcct gaatgagatt gcggaagtca ccctcaaaac cgcgcaaccc gttgttttcg
     2461 acacctatgc ccaaaactgg actactggtg ccggggtgct tgtcgacgcg cagacgcaca
     2521 tgactgcctg cgcggtcatg aatgcaggca gcgcagaatg aaaggtacgg ttacgattgc
     2581 cggcgcgggc ccgggagagc tggaacacct gacagtcggg gcactgcgcg ccatacacgc
     2641 agcggatgtt atactctatg acgccttgat tggcgactct atcatcaggg agtttccttc
     2701 gaaggcaaaa accatttttg ttggtaaacg ctgcggtaac catgcctaca cgcagacgat
     2761 gattatcgct gcaatgatcg aacacgcgat ggcaggccgt aatgtgctgc gcctgaaagg
     2821 tggcgatccg gcgatctttg cgcatctatc agcagaactc gaagccctga agaatctggg
     2881 aatcgagaca cgcatattac cgggagtcac cgcaatgctg accgctgcag ccgacctggg
     2941 ccgtccgctc acgacgcgtg gcagcaaccg gcacatctgg atcac
//
LOCUS       DAOQJF010000385         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_22639_length_2961_cov_5.426703, whole genome shotgun sequence.
ACCESSION   DAOQJF010000385 DAOQJF010000000
VERSION     DAOQJF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22639_length_2961_cov_5.426703"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..827
                     /gene="fliP"
                     /locus_tag="PLF85_15415"
     CDS             69..827
                     /gene="fliP"
                     /locus_tag="PLF85_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577827.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNE21109.1"
                     /translation="MKLAIRIALLSLLLGGAYSLAAQQVPIPRVDFNISQAKSPQQVA
                     LSLQILLLLTILSLAPAIILMLTSFTKIIIVFDFMKRALSLQNMPPNQVMVSLALFIT
                     FFIMAPTFDEMNKKAITPYLDGQISNTGFFKEAAAPLRKFMIRQIGKEGIKEIALFVR
                     LSKSERPKKIDDVPMQVLIPAFMLTEIKKAFIIGIYLFIPFLIIDMVVASVLMSMGMM
                     MLPPVMISLPFKIILFVLVDGWTLLTYEIVRSYG"
     gene            complement(811..>2961)
                     /locus_tag="PLF85_15420"
     CDS             complement(811..>2961)
                     /locus_tag="PLF85_15420"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE21110.1"
                     /translation="QQLQATYLMAQLDALPGGEGLKDAAVRLVEQAARHSAIQAAYLK
                     QLVLSRPYTSGTEIARAISSLRNQQLYQAAKLRQMTLPEIAAKKEAVLRTLDDRFPGL
                     AAAAQTSLSMPPDQLRSALTLALTPWRFEGERLYRGEIFFKDKFPAHFVAYPFIDAAK
                     RRVIEVAYPYLEYREAIARHAWPLVSIILFAYLFILIGFSLFFRGALMKPINALVGGL
                     KEIHNNNFDARVKVQVEDEIGFMAKSFNKMARSIKAGRMRLQKYAEQLEEKVKERTQE
                     LQNTLKDVQALKAQQDGDYFLTTLLLKPLGVNEVDSRFIGIESFVRQKKKFEFRHWSK
                     DIGGDINISHVIELEGKRYIAFINGDAMGKSIQGAGGALVLGSVFHTIIERTQATEAM
                     RQLAPERWLKNAFIELHRVFESFDGSMLVSGFFGLIDEKTGLMYHILAEHPLPVLYRN
                     GKASFVDNDRMLRKLGTTGVEGTLVIATTQLEPGDILLVGSDGRDDIILGHDEHGGRI
                     INEDETLFLSIVEESGGDMQAIVRALESHGEIMDDLSLMRIERYREEHNNHRAFDYTG
                     VLQGVKRDIRQGATAKAIQRIEAYLQHDAFYPEAVKNLAQIYYQSKNYEKAANYAQDY
                     LWLKPSDAEFVYFTALCFRRIRDYRKAIDLSERLRLREVPMAKNLALLTDLHLRTGNK
                     TRAETILAELVAHDAEFSSIQILKRKLADATRSS"
BASE COUNT          667 a          825 c          759 g          710 t
ORIGIN      
        1 gcgccacgac tttcgctcga acgcctgcgc aaactcaagt cagaaaagtt cgactggcgg
       61 gacaaagcat gaagctggca attcgtatcg cgctgctctc gctgctctta ggcggagctt
      121 attccctggc ggcacagcag gtaccgattc cgcgagtgga tttcaatatt tcacaggcca
      181 agtcgccgca gcaggtggct ctttcgctgc agattctgct gctgctgaca attctctcgc
      241 tggcgcctgc gatcatactc atgctcactt cgttcacgaa gatcatcatc gtgttcgatt
      301 tcatgaaacg cgcgctcagc ctgcagaaca tgccccccaa ccaggtgatg gtctcgctcg
      361 cactctttat cacctttttc atcatggcgc cgaccttcga cgagatgaac aagaaggcga
      421 tcacaccgta cctcgacggc cagatcagca ataccggttt ttttaaagaa gccgcggccc
      481 cgcttcgcaa attcatgatc cggcagattg gcaaagaggg catcaaagaa atcgcgctgt
      541 ttgtgagact gagcaaaagt gagcgaccca agaagatcga cgatgtgcca atgcaggtat
      601 tgattcctgc gttcatgctg acagaaatca agaaagcctt tattatcggt atctacctct
      661 tcataccgtt cctgatcatc gacatggtcg tcgcgagcgt gctcatgtcg atgggtatga
      721 tgatgctgcc tccggtcatg atctcgttac ccttcaagat catcctcttt gtactggtcg
      781 atggctggac attgctgacg tacgaaatcg tcaggagcta cgggtagcgt ccgcgagctt
      841 acgtttgagt atttgaatac tcgagaactc cgcatcatgc gcgaccagct ctgccagtat
      901 cgtctcagct cgcgtcttat tgccggtccg caggtgcagg tcggtcagca atgccaggtt
      961 tttcgccatt ggcacctctc tcaaccttaa ccgctcggaa agatcaatcg ccttgcggta
     1021 gtcgcgtata cggcgaaagc acagcgcggt aaaatagaca aattcggcat ccgatggctt
     1081 cagccagagg taatcctgcg catagttcgc agccttctca taattcttgc tctggtagta
     1141 aatctgcgcc aggtttttga ccgcctcggg gtagaatgcg tcgtgctgca ggtaggcctc
     1201 aatacgctga atcgccttgg ccgtagctcc ttgccggata tcgcgtttga ctccctgcaa
     1261 tacaccagtg tagtcaaatg cccggtggtt attatgctct tcgcgataac gctcgatacg
     1321 catcagcgac aggtcatcca tgatttcacc gtgggactca agcgcacgga caattgcctg
     1381 catatcaccg ccagactctt caacaatgct caggaataac gtctcgtctt cattgatgat
     1441 gcggccgcca tgttcgtcgt ggccgagaat gatatcgtcg cggccgtctg aaccaacgag
     1501 taatatgtca cccggttcca gctgcgtcgt ggcaatgacc agcgtgcctt cgaccccggt
     1561 tgtgcccagc ttgcgcagca tgcggtcatt atcgacgaag ctcgccttgc cgttacggta
     1621 caacacagga agggggtgtt cggcaagtat gtggtacatg agcccggtct tctcatcgat
     1681 caggccaaaa aagcccgaaa cgagcatcga accgtcaaaa ctctcgaaga cgcggtgcag
     1741 ttcaatgaag gcattcttta gccagcgttc cggtgcaagc tgccgcatgg cttcggtcgc
     1801 ctgtgtgcgt tcaataattg tatggaaaac cgaaccgagg acaagcgccc cgccagcccc
     1861 ctggatcgat ttacccatcg catcgccgtt gatgaacgca atatagcgct taccttcgag
     1921 ttcaatgacg tgcgaaatat tgatatcacc gccgatatcc ttgctccagt gcctaaactc
     1981 gaattttttc ttctgcctga cgaaagattc aatgccgata aaccggctgt ctacctcgtt
     2041 gactcccagt ggcttaagca acagcgtcgt aagaaaatag tctccatcct gctgcgcctt
     2101 gagcgcctgc acgtctttaa gagtattctg cagttcctgc gtgcgctctt ttactttttc
     2161 ttccagctgc tccgcgtact tctgtaatcg catacgcccc gccttgatag agcgcgccat
     2221 cttgttgaag cttttggcca taaagccgat ttcatcttcc acctgtacct tgacgcgcgc
     2281 gtcaaagttg ttattatgta tttctttaag cccgccaacg agagcgttaa tcggcttcat
     2341 aagcgcgccg cgaaagaaga gagagaatcc gatcagaatg aacaggtagg caaaaagaat
     2401 gatgctgacc agcggccacg catgccgcgc aattgcctcg cggtattcaa ggtaaggata
     2461 cgcgacctcg attacgcggc gctttgcagc atcaatgaaa gggtacgcca caaagtgcgc
     2521 gggaaatttg tctttaaaaa agatttcacc gcggtagagt cgttcaccct cgaaccgcca
     2581 cggggtaagc gcgagggtta gcgccgacct cagttgatcg ggtggcatac tgagcgatgt
     2641 ttgcgcggcg gcggccaacc cgggaaaacg atcgtccagc gtccgcaata cagcttcttt
     2701 ttttgcggca atctcgggca gcgtcatctg ccgcagcttg gcagcctggt agagctgctg
     2761 gttacgcaac gaagaaattg cgcgcgcgat ctctgttccg ctggtgtatg gacgcgaaag
     2821 caccagttgt ttcaggtagg cggcctggat agcagaatgg cgcgcagcct gttcaacaag
     2881 gcggactgcc gcatctttca ggccttcacc gccgggcaac gcatcgagct gtgccatgag
     2941 gtaagttgcc tgcaactgct g
//
LOCUS       DAOQJF010000386         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_22824_length_2947_cov_5.789419, whole genome shotgun sequence.
ACCESSION   DAOQJF010000386 DAOQJF010000000
VERSION     DAOQJF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22824_length_2947_cov_5.789419"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="PLF85_15425"
     CDS             <1..2010
                     /locus_tag="PLF85_15425"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE21111.1"
                     /translation="PPVYKWGSPFLEVINISNIIFAALFQGGFAYYFTWAASKAEQSL
                     EDEHAAQSAFFQNISHELRTPLTLILGPADSALRRKAHLNNDELEMVVSNANRLSQLV
                     NRLLDLQKVTAGKMVLNLEPRDLAAYVKATGEAFKPYAESRQIRLVVEVPGTPVAVEY
                     DEQEIDKCLYNLLSNAFKFTPRGKSITLVLQAVDGWAEVAVRDEGVGIDAEQQKRLFS
                     RFGISRASLTREQEGTGLGLALVKELVELHKGYVGVESEVNRGSRFFFRLPLSDKTEL
                     LQSTTLQPRVPEAAAKRPADTAQAAQNGARILVVEDNPDLRSYIGAILQRAGHHVSFA
                     ENGDEGRVKFSELAPDVVITDLMMPKVSGLGLIQYIRTESQMPSVPVILLTAKASVET
                     RQEVIQHGANHFLSKPFRDSELLDVLHNVLQLKAPERRLTKERAEAMALQKKLLPASI
                     PQIIGFQMAVTFRPAEGIAGDLYDFFPLADGRLGVLIADVSGHGIAAGMIGSMAKLAL
                     GLPGAPLGEPARLLQFINGILTERTASYFLTAIYLVVNPSTGEVILARAGHPPLYWLQ
                     NGNISALKPAGTALGWDKEAGWIEQGLTMMKGDRLILVTDGIIETTGYTGALRDEEEF
                     RKLLRENSGVNLAEFGEALMQSASKIAGRGTFDDDITIIAIERTT"
     gene            2072..2599
                     /locus_tag="PLF85_15430"
     CDS             2072..2599
                     /locus_tag="PLF85_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNE21112.1"
                     /translation="MVYALGEQNPALAASAWVAPNATVIGDVTLGERSSVWYGCILRG
                     DVHSIRVGSDTNIQDATVIHVTGGKFATTIGDRVTIGHRVLVHGATLLDDSFIGMGAT
                     LLDGATVEPFGFVAAGALVPPGMVVKSGWLALGSPAKLVREIKPAEREMMLAIWPRYV
                     EHADRHRRELRPVQG"
BASE COUNT          624 a          805 c          886 g          632 t
ORIGIN      
        1 ccgcctgtct acaagtgggg tagtccgttt cttgaggtca tcaatatctc taacattatt
       61 tttgctgcgc tgttccaggg agggtttgcc tactacttta cctgggccgc gagcaaggcg
      121 gagcagagcc ttgaagatga gcatgctgcg cagtcagcct ttttccagaa tatatcgcac
      181 gagctgcgca ctccgctgac gctgattctc gggcccgcag actctgcact ccgccgtaag
      241 gcgcacctga acaacgacga actggaaatg gtcgtcagca atgcgaacag gctgagccag
      301 ctggtgaacc gcctgctcga cctgcaaaag gttactgccg gcaagatggt actcaacctg
      361 gagccgcgtg acctggcagc ttatgtcaaa gccaccggcg aggcatttaa accgtatgct
      421 gaatctagac aaatcaggct ggtcgtcgag gtgcccggga caccggtggc tgtagagtac
      481 gatgagcagg aaattgacaa gtgcctttat aatttgctct cgaatgcatt caaatttacg
      541 cccaggggaa agtctattac gcttgttctg caggcagtcg atggctgggc cgaagtggcc
      601 gtgcgggatg aaggtgtcgg tatcgacgcc gaacagcaaa agcgcctgtt ctcgcgtttc
      661 ggtatctcgc gcgcttcgct tacccgtgag caggagggca ccgggcttgg gctcgcgctg
      721 gtgaaagaac tcgtcgagct gcataaaggt tacgtcggtg tcgagagcga agtcaatcgc
      781 ggttcgaggt tcttctttcg cctgccgctg agcgacaaga ccgagcttct gcagtccaca
      841 accttacagc cgcgtgtccc cgaggctgcc gcaaaacgac cggccgatac tgcacaggca
      901 gcgcagaacg gtgcacgcat tcttgtagtt gaagataatc ccgatctgcg cagctatatc
      961 ggggccattc tacagagggc aggccaccat gtgtccttcg ctgaaaacgg cgacgaaggc
     1021 cgcgtgaagt tcagcgagct agcccccgat gtagtgatca ctgatctcat gatgcccaag
     1081 gtgagcggac tgggtctcat acagtatatc cgtaccgaat cgcagatgcc gtcggtgccc
     1141 gttattctgc tgactgccaa agcatcggtc gagacccggc aggaagtcat acagcacggg
     1201 gcgaaccatt ttctttcaaa gccgttccgc gattcggagc tgcttgacgt attgcacaat
     1261 gtactgcagc tgaaagcccc ggagcgtcgg ctgaccaaag aacgcgccga ggcgatggcc
     1321 ctgcagaaga aactgttgcc ggcatcgatt ccgcagatta tcggttttca gatggcggta
     1381 acttttcgcc cggccgaagg tatcgcgggt gacctctatg attttttccc gctggcagat
     1441 ggcaggttag gtgtgttgat tgccgatgtc tcgggacacg gcattgctgc cggtatgatc
     1501 ggcagcatgg cgaaactggc gctggggttg ccaggtgcac cgctgggcga gccggcgcgc
     1561 ctgctgcagt tcatcaacgg tattctgact gaacgcaccg caagttactt cctgacggcg
     1621 atctatctcg tggtgaatcc ctcgactggt gaagtaatac tcgccagggc gggacatccc
     1681 ccgctgtact ggctgcaaaa tggaaacatc agcgcgctga aaccggcggg tactgcgctg
     1741 ggttgggata aagaagccgg ctggatcgag cagggattga cgatgatgaa gggcgacagg
     1801 ctgattcttg tcaccgacgg tatcatcgaa accactggct ataccggcgc cctgcgcgac
     1861 gaagaggagt tcaggaagct gctgcgtgaa aactccgggg tcaacctcgc agagttcggc
     1921 gaagcgctga tgcagagcgc cagcaaaatt gccggccgtg gtacttttga tgacgatatc
     1981 acgatcattg cgatcgagcg caccacgtag cgtgcggcag ttgcggtgtg aatcgcactt
     2041 gtatcggcat atcagggccc ggtgatggcg catggtgtat gcactcggtg aacagaaccc
     2101 cgcgctggcg gcttcagcct gggtggcccc gaatgcaacc gtgatcggcg acgtgacgct
     2161 cggcgagcgc agttcagtct ggtacggttg tattctgcgc ggtgacgtgc attcgattcg
     2221 cgttggatct gatacgaata tccaggatgc cacagtgatc cacgtcaccg ggggcaaatt
     2281 tgccacgacg atcggcgatc gcgttacgat cggccaccgg gtgctggtac atggtgcaac
     2341 gctgctcgac gatagtttca tcggcatggg tgcaacgctg ctcgacggag ccaccgttga
     2401 accctttggt tttgtcgcgg ccggtgccct cgtaccgccc ggtatggtgg tcaagtcggg
     2461 ctggctggca ctcggcagcc cggcgaaact cgtccgcgag atcaaacctg cagagcgcga
     2521 gatgatgctc gcgatctggc cgcggtacgt cgaacatgct gaccggcacc gccgggagct
     2581 caggcctgtc caaggctagg aatactctga aaaagccgcc acaaatggcc tgttttcacg
     2641 cgaagtccgc gcctgcccag cgcggttcgc gactttttca gacattccct gggtcacgaa
     2701 tcactcgccg aaaattgctg cacgcagtat ctttagagca ttgttcggtg gcgccggcga
     2761 gcgcctgaag gagttatatg aagatctaca gtctggtgcg cgagcagctt gtcagggcac
     2821 cgctaggtga aacctgggcg tttttttcca acccggcaaa cctgaaaaga atcactccat
     2881 ctgcaatggg atttcatatt ctcacgcccg atttgccgca ggagatctat gcaggacttt
     2941 tcattga
//
LOCUS       DAOQJF010000387         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_22981_length_2935_cov_3.871181, whole genome shotgun sequence.
ACCESSION   DAOQJF010000387 DAOQJF010000000
VERSION     DAOQJF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22981_length_2935_cov_3.871181"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1267)
                     /gene="pheS"
                     /locus_tag="PLF85_15435"
     CDS             complement(215..1267)
                     /gene="pheS"
                     /locus_tag="PLF85_15435"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNE21113.1"
                     /translation="MADLIAEIQALEGKAAQAIAAAADAEALALVTQEFTGKKGALTG
                     IMKQLGALTPEQRGEVGKAANAFKAKLDELLSAKEAQLTELKFSALAETEFLDLSVTP
                     PQFSAVGKTGDYTGHIHPITQTQRRLEQIFTSMGFSIVDGPQIEDDYHNFGALNFTDD
                     HPARDMQDTFYTVTGHLLRTHTSTVQIRALESMKPPLRIIAPGRVFRYEEVDASHEHT
                     FYQMEGMIVDRKVSVGNLLHLMQTLLTEVFGKQVDVRLRPGYFPFVEPGFELDMKCLV
                     CNGEGCSVCKHSGWVEVLPCGLVHPNVLRSAGLDPAEWQGAAFGLGLNRLVMMQHGIE
                     DIRHFMAGKLEFLRQF"
     gene            1376..1924
                     /locus_tag="PLF85_15440"
     CDS             1376..1924
                     /locus_tag="PLF85_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21114.1"
                     /translation="MQTSRLIAVLLALSTTLAAAEKPLTLGDLGIAQPSGANTALQKD
                     LETRSTMLQWHQWLGIAAILPMAGTYILGTQAQNDSATRDLHMALGVSTAALYFTSAS
                     FAIFAPKPDGIKDTGSTRWHRYLSYIHLPLMIAVPILGNMARSQAENGERTSGAASLH
                     GPAASLLLGTYLVSVSVLVFNF"
     gene            1927..2487
                     /locus_tag="PLF85_15445"
     CDS             1927..2487
                     /locus_tag="PLF85_15445"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNE21115.1"
                     /translation="MARSRTYLPLIAAGLASAVVATAPVELAGNWQLKEATTTYKVSH
                     PLKTARGISRSAKGRVRCDKSSCEALLAVDVKSFDSGDSNRDLHMLETVKGALHPVLT
                     VRSRFAPRLAAGGQLTVDLEIELAGQKATVRGVNLQTELLTNRSLKVSGAFIIHLSAF
                     GIERPSLLGMSIKDPVPITFISTWER"
BASE COUNT          646 a          841 c          817 g          631 t
ORIGIN      
        1 cgatcagcgc cacaggaatt ttcgccatgt ggtccatgaa aggttcgaaa ccttcgacct
       61 cgcaggtctt catggtaagc aggttcgcga gctcagccgg cgttgtgccc gagaggtcga
      121 caaattcgtt tagccagttg tatgatagtt tcatgttttt tctcctgcaa tgcggcacat
      181 gctgctgcgc agcatgtgcc gcattgcatt tattctagaa ttgtcttaaa aattcgagtt
      241 tgcccgccat gaagtggcgg atgtcttcaa taccgtgctg catcatgaca aggcggttta
      301 agcctaaacc aaacgcggct ccctgccact cggcgggatc aagaccagcg gagcgcaaca
      361 cgttcgggtg cacgagaccg cagggcagga cttctaccca gcccgaatgc ttacaaacgc
      421 tgcatccctc gccgttgcac acaaggcact tcatatcgag ctcgaagcct ggctcaacga
      481 aaggaaagta gccggggcgc aggcgcacat cgacctgttt gccgaagacc tcagtgagca
      541 gagtctgcat gaggtgcagc aggttaccga ccgaaacctt gcggtcaaca atcataccct
      601 ccatctggta gaaggtgtgc tcgtgcgaag catcgacctc ttcatagcga aacactcggc
      661 cgggtgcaat gatgcgcagt ggcggtttca tgctttcgag cgcgcgaatc tgcacggtcg
      721 aagtgtgtgt gcgcaacagg tgccctgtta ctgtgtaaaa tgtatcctgc atgtcgcgcg
      781 cgggatggtc gtcggtaaag ttgagcgctc caaaattgtg gtagtcgtct tcaatctgcg
      841 gaccatcgac gatcgaaaag cccatcgagg taaagatttg ctcgagacgc cgctgcgtct
      901 gtgtaatcgg gtgtatgtgc cccgtgtaat cgcctgtttt gccgacggct gaaaactgcg
      961 gcggggttac ggacagatcg agaaactcgg tctctgccag agcagaaaat ttgagctccg
     1021 tcagctgcgc ttctttggct gaaagcagtt catcgagttt cgccttgaac gcattggcgg
     1081 ctttaccaac ttcgccgcgc tgttcgggag tgagcgcacc gagctgtttc atgatgcccg
     1141 tgagtgcgcc ctttttgccc gtgaattcct gtgttaccag tgcaagcgct tcggcatcgg
     1201 cagcggcggc gattgcctgc gcggccttgc cttcgagagc ctgaatttca gcaattaaat
     1261 cggccatttg ccgataaaat aggcttttga gacaccgcgt caaggtggtg ggggattggg
     1321 acacacccgt gccgaatcgg cttgacgagc tgcgaaaaac ccgagctccg aaggcatgca
     1381 aacttcccgc ctgatagccg tactgctggc actgagcaca acactcgctg cagcagaaaa
     1441 gccgctcacg ctcggcgatc tcggcattgc acaaccttca ggagccaaca ccgcattgca
     1501 gaaagatctc gaaacgcgca gcaccatgct gcagtggcac cagtggctcg gtatcgctgc
     1561 gattttgccg atggccggaa cctacatttt gggcacgcag gcacagaacg attcagccac
     1621 ccgtgatctg cacatggccc tgggagtttc gaccgcggcg ctctatttta cgtcggcgag
     1681 ttttgccatc tttgcgccca agcccgatgg catcaaggac acaggcagca cacgctggca
     1741 ccgctatctg agttacattc acctgccgct gatgattgcg gtgcccattc tcggcaatat
     1801 ggcgcggtcg caggccgaaa atggcgagcg cacgagcgga gctgcctcgt tgcatggccc
     1861 cgcggcatcg ctgctgctgg gtacatacct cgtgtcggtt tcggtgcttg tcttcaactt
     1921 ctgagcatgg cacgttcacg cacatacctc ccccttattg cagcaggcct cgccagcgcg
     1981 gtagttgcca cagcacctgt agaactcgcc ggcaactggc agctcaaaga ggccacgacg
     2041 acctacaagg tgagccaccc gctgaagaca gcccggggta tctcacgatc tgccaagggg
     2101 cgcgtgcgct gcgacaagag cagctgcgaa gccttgctgg cggtcgacgt aaagtcgttt
     2161 gattcggggg acagcaaccg cgacctgcac atgctcgaaa ccgtgaaggg cgccctgcac
     2221 ccggtgctga ctgtacgtag ccggtttgcg ccccggctgg cagcaggtgg gcagcttacg
     2281 gtagacctcg aaattgagct ggcagggcag aaagcgacgg tcaggggagt taacctgcag
     2341 acggagttac taactaacag aagcctgaaa gtctccgggg catttattat tcacctttcg
     2401 gcattcggca ttgaacggcc gtcattgttg ggaatgtcca tcaaggaccc tgttccgatc
     2461 acctttattt cgacctggga aagataatcc tgccgcgggc gcaggtgccg ggtcccccct
     2521 cgcattcggg caaaaagggc ttggagtaca ctgaatagtc gcccgtcgca cctctgcagc
     2581 gcggtccgcg tcattttcag ccattccctt ggtagttact aaagcccacg gaccgcatac
     2641 ctgaacccct gtgccccctt cggccaattg cgcgccgcac aataaaaagt tgacactccg
     2701 tctatttttg cttattttta acttatagtg cattgcacca aatcgcaaga tttggccacg
     2761 ggtgcgaacc agacggcaca ggccatcgga acaccccaag gagacggcag aagagtcaac
     2821 gcgtttgcct gcgaacgcct aggctgtccg ccgtctccaa gatacctcgt gtatcatagg
     2881 aggaatatgc aaagaaccaa aggagcaaca ccgaccgcga aacttacctt catta
//
LOCUS       DAOQJF010000388         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_23008_length_2933_cov_4.823836, whole genome shotgun sequence.
ACCESSION   DAOQJF010000388 DAOQJF010000000
VERSION     DAOQJF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23008_length_2933_cov_4.823836"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1354
                     /locus_tag="PLF85_15450"
     CDS             149..1354
                     /locus_tag="PLF85_15450"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF006553.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNE21116.1"
                     /translation="MTKQVQEAYIVATTRTPVGKAPRGMMRNVRPDDMLAHVIKGALA
                     QVPALDVNLIEDCVVGCAFPEAEQGLNMARIGVLLAGLPNTVGGVTINRYCSSGINAV
                     QMAADRIRTGEADVVIAAGAESMSMVPMMGNKVSINPEVFAKDENFAIAYGMGLTAEK
                     VAQQWNVSRADQDAFAVESHRRAAAAIAAGEFKDEITPLDVTYRIPNLATGEVEYVKK
                     TLTNDEGPRPDTTLEGLAKLKTVFDAKGSVTAGNSSQMSDGAGAVILVSEKILKQFNL
                     TPLGKFISFAVKGVPPAIMGIGPKEAIPAALKIAGLSKSDIAWMELNEAFAAQALAVI
                     RDLDLDTSLINPLGGAIALGHPLGATGAIRTATLLHGMRRKGMAGKYGMVTMCIGTGM
                     GAAGIIQAL"
     gene            1518..2762
                     /locus_tag="PLF85_15455"
     CDS             1518..2762
                     /locus_tag="PLF85_15455"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE21117.1"
                     /translation="MSKKSVKLLASVLAMAVSPLVGAAGVSDDVVKIGVLTDMSGVYA
                     DVGGKGALVAAQMAVKDFGGKVLNKRIEVVYDDHKNDVDTGLAIAKRWFEKDRTKVDV
                     VVELMNSQVATAVSKYAAEQKRIAVKTGAATSALTNDECTRYTIDWVYDSYAVSLGTL
                     RELLADGIKTYTIMVTDYGDKTDKAFSKDIFIAANMVRKKGGKIFFPPIKVPASSTDF
                     TPYLLKAQEQGANGILFAQAGGDFVNAVKQAREFGLFSETQEPVGMVVFDSDIKALGL
                     DMAQGMKFTTGFYWDRDNDSREWSRRYYAQMKKMPTSSQAGVYSAVMHYLRAVQSSGT
                     DSADDVMSKMKSTRINDFFAKDGYVREDGRMIHDMYLAEVKKPSESKGEAQWDLLKIK
                     RTIKGENIFRPLAQSACPLVKK"
BASE COUNT          703 a          663 c          870 g          697 t
ORIGIN      
        1 cgaaatcgca agaacggatc ttctacatgc tgcaaaacaa caaaccgctc cgcaattgat
       61 acggacgcaa gtatttacct ccgccgcatg ttggcggagg cctaaaccaa ggtttgttgt
      121 acagatactg aatttggaga ttgtttaaat gaccaagcaa gttcaagaag cttacatcgt
      181 cgcgacgacc cgtaccccgg tgggtaaggc tccgcgcggc atgatgcgta acgtgcgtcc
      241 ggatgacatg ctggctcacg taatcaaggg tgcgctggct caggtgccgg cgctggatgt
      301 gaacctgatc gaagactgtg ttgtgggttg tgcgttcccg gaagcggaac aaggcctgaa
      361 catggcgcgc atcggcgtgc tgttggccgg tttgccgaac acggtgggtg gtgtaaccat
      421 caaccgttac tgctcgtccg gtatcaatgc cgtgcaaatg gctgcagacc gtatccgtac
      481 cggcgaagcc gatgttgtca tcgctgccgg cgcagaatcg atgtcgatgg taccgatgat
      541 gggaaacaag gtttccatca atccggaagt gtttgccaaa gacgaaaact ttgccatcgc
      601 ttatggcatg ggcctgacgg cagaaaaagt tgctcagcag tggaatgtat cgcgcgcaga
      661 tcaggatgcg tttgctgttg agtcgcatcg ccgtgctgct gctgctatcg ccgcaggtga
      721 attcaaggat gaaattactc cgctggacgt gacctaccgt atcccgaatc tggctactgg
      781 cgaagtggaa tacgtcaaga aaaccctgac caacgacgag gggccgcgtc cggacacgac
      841 tctggaaggt ttggccaagc tgaagactgt atttgatgcc aagggttcgg tgactgcggg
      901 taacagttcg cagatgtccg atggggctgg tgcggtaatt ctggtttcgg aaaagatcct
      961 gaagcagttt aacctgaccc cgctgggcaa gttcatttcc ttcgcagtta aaggcgttcc
     1021 accggcaatc atgggtatcg gcccgaaaga agcgattccg gctgcgttga agattgcagg
     1081 tctgtcgaag agcgatatcg cctggatgga gctgaatgaa gcgtttgcag ctcaggcgtt
     1141 ggcagtaatc cgtgatctgg atctggatac cagcctgatc aatccgctgg gcggtgcgat
     1201 tgccttgggc catccgctgg gtgcaactgg cgcgattcgc actgcgaccc tgttgcacgg
     1261 tatgcgccgt aaaggcatgg caggaaagta cggtatggtg accatgtgta tcggtaccgg
     1321 tatgggcgcc gcaggcatca ttcaggcact gtaatcaaga tagtatgttg tataaagaaa
     1381 ggggagacgg gtgtcttccc ttttttttga aacataattc gtgtaggccg gattcgcttg
     1441 atgcgatatc tgctgctaat ataagagcaa actgaaatac ccgatattgg gcaatcactc
     1501 tggatgaggg actacagatg agcaaaaaat ccgtcaagct tcttgcttct gtcttggcga
     1561 tggcagtttc gccattggtt ggggctgcag gagtgtcgga cgatgtcgtc aagattggtg
     1621 tcttgactga catgtcaggg gtgtatgccg acgtcggcgg caaaggcgcg ctggtcgccg
     1681 cgcaaatggc agtcaaggac tttggcggca aggtgttgaa caaacgtatt gaagttgtgt
     1741 acgacgatca caagaatgat gtcgacactg ggcttgcgat tgccaaacgc tggtttgaga
     1801 aagatcgtac caaggttgat gtggtcgtcg agctgatgaa ctcacaggtt gcaacggccg
     1861 tatcgaaata cgcagcggaa cagaagcgga ttgcggtcaa gaccggggct gccacatcgg
     1921 cgttgaccaa cgacgaatgt acacgataca ccatcgactg ggtttatgat tcctacgctg
     1981 tttcgttggg cactttgcgg gagctgctgg ccgatggcat caagacttac accatcatgg
     2041 ttaccgatta cggtgacaag accgacaagg cgttttccaa ggacatcttt atcgctgcca
     2101 atatggtgcg caagaaaggc ggcaagattt tcttcccgcc gatcaaggtg ccggcttcgt
     2161 caaccgattt taccccatat ctgctgaaag ctcaggaaca gggagctaac ggcattttgt
     2221 ttgcccaagc gggtggggat tttgtcaatg cggtcaagca agcaagggaa ttcggcctgt
     2281 tctcggaaac gcaggaaccg gtagggatgg tggtttttga ttctgatatc aaggcgcttg
     2341 gccttgatat ggctcagggg atgaaattta ccactggctt ttattgggat cgtgacaatg
     2401 acagccggga atggtcgcgc aggtattatg cgcagatgaa aaaaatgcca acatcttctc
     2461 aggctggtgt gtattctgcc gtgatgcatt accttcgtgc ggtacagtcg tccggaaccg
     2521 attcggctga tgatgtgatg tcgaaaatga aatctacccg gattaacgat ttctttgcca
     2581 aagatgggta tgttcgtgag gacggccgga tgattcacga tatgtatctg gctgaagtca
     2641 agaagccgag tgaatcaaag ggcgaggccc aatgggatct gctgaaaatc aaacgtacga
     2701 tcaaaggtga gaacatattc cgcccgctgg cccagagtgc atgcccgctg gtcaagaagt
     2761 aactatgcaa atgccataaa attgtataga atcggtgctc taattcgagc ccacccgtga
     2821 gctaatcagg gcgcgggtgg gctttcttat attaagtgta ttttcacgag gggaatggaa
     2881 tgaaaaagct ggttgcaatg gctgctctga tgggggcgac ttcgggtgcg ttt
//
LOCUS       DAOQJF010000389         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_23090_length_2927_cov_4.863858, whole genome shotgun sequence.
ACCESSION   DAOQJF010000389 DAOQJF010000000
VERSION     DAOQJF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23090_length_2927_cov_4.863858"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PLF85_15460"
     CDS             <1..993
                     /locus_tag="PLF85_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE21118.1"
                     /translation="IALTERLARTQAENQLHTALIRTILQSDPVTLAEFLQDLEGDLE
                     AAREYLDAAAQGEISALEQLFRVVHSIKGNASILEFSLLSSRAHAMESEIDQQLKPKG
                     AGGAGMPRLREQLEELERLSLEVSNWLGRIEIFHENYSRQTSGDYLQRALSTVFARAL
                     QAEGKEARLQVSGLDLARVPARYRKNLKDLLVQLARNAAVHGFETPSARQSKGKPAQG
                     LLKISAIGAAEQVVVVIEDDGAGIDTATLGAKLIKRGTLTEAAFAAMPQAERLNLVFA
                     SGLSRLGDATHYAGRGMGMSIVADAVAQMQAIIRTESEPDRFTRFTVEIPATTM"
     gene            complement(1012..1410)
                     /locus_tag="PLF85_15465"
     CDS             complement(1012..1410)
                     /locus_tag="PLF85_15465"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HNE21119.1"
                     /translation="MRPSGSILTLLLVCTACGQPAKESAEQNRLRNQVYFSVDDPGRW
                     KGFAKDHDPQVKVSGGKIEVEVPFTAEMGHYTEVIFIQDMAQREVALRTFKRGEKAHA
                     TLSIPLEKMPGAAVITKCNLHGMWKKSIVQ"
     gene            1612..>2927
                     /locus_tag="PLF85_15470"
     CDS             1612..>2927
                     /locus_tag="PLF85_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21120.1"
                     /translation="MLSLRISVLSLLTLITINCARIDELGSRFFKKEGAITARITATN
                     LAGRNLSLQINQDTFTTSVAQTVVSTNVPRGENFTITIAAQPVLPAQTCTLTSPTTGV
                     ASGDLLEITLSCTTQKFTISGMTTGYAGSGLQVQLNGGEIVTVTGSSFSFTTLVDDNS
                     TYNVQTKTNPTGPAQICTAQKNVGLVTGANISDVSINCSTVTYSIGGFVNGLAAGQSV
                     TLQVNGTQSTVRSTDGFFTFTNKLADQTEYQITVLTQPAGQSCSVGRGAGILNGEGAF
                     SALIQCSSTTHTISGNLTGLTGGFVTLVSSSGEYRPLTANGAFVFTQTLATGTDYVVE
                     VFRNPAAPAQACTVTNATGTISANVNNINVSCASTGYSIGGNSTVSGLTSAGLVLRLN
                     GSSETVVPANATSFSFSETVAAGSNYTVTIAAQPTGQKCTLSNASG"
BASE COUNT          682 a          778 c          833 g          634 t
ORIGIN      
        1 attgcgctca ctgagcgcct ggcgcgcacg caggcagaaa accagctcca tacagcactc
       61 atccgcacga ttctgcagtc tgacccggta acgctggcgg agtttctgca ggatctcgag
      121 ggcgatctcg aggcggcgcg cgaatacctg gatgcagcgg cacagggaga aataagcgct
      181 cttgagcagc ttttccgcgt tgttcattct atcaagggta atgcttcgat actggaattt
      241 tcgctgctct cgtcgcgcgc gcatgccatg gaatcagaga tcgaccaaca gctgaaaccg
      301 aaaggggccg gtggagcagg tatgccacga ctgagggagc aactcgaaga gctggaaagg
      361 ctcagtctgg aagtcagcaa ttggctgggg cgtatcgaaa tctttcatga gaattacagc
      421 cgccaaacct caggcgatta cctgcagcgc gccctttcta cggtctttgc ccgagcactg
      481 caggccgaag gtaaagaagc gcgcctccag gtctctggtt tggatttggc acgtgtcccg
      541 gccaggtaca ggaaaaatct taaggatctg ctggtgcagt tggcgcgcaa tgctgctgtg
      601 catggttttg agaccccatc tgcaaggcaa agtaagggta aacctgcgca aggtttgctc
      661 aagatttcag cgattggggc agcagagcag gttgtcgtcg taattgaaga cgacggggca
      721 gggatagaca ccgctacact tggggcaaag cttatcaagc gcggaacgct gactgaagcg
      781 gcgtttgcag cgatgccgca ggccgagcgg ctgaacctgg tatttgcgtc gggtctatcg
      841 agactgggtg atgcgacgca ttatgcgggc cgtggcatgg gaatgagcat tgttgccgat
      901 gccgtggcgc agatgcaggc gattattcgc accgaatcag aaccggaccg gtttacgcgg
      961 tttacggttg agattcctgc tacaaccatg tgagtcgggc atagtctaaa tttactgaac
     1021 gatcgacttt ttccacatgc cgtgcaggtt acatttggta atcaccgcag cacccggcat
     1081 tttttcaagg gggattgata aagtcgcgtg ggccttttca ccgcgtttga aggtgcgtag
     1141 agccacttct cgctgcgcca tatcttgaat aaaaatcact tctgtatagt gtcccatctc
     1201 tgcagtaaag ggtacctcca cctcaatctt accgccgctc accttcacct gagggtcatg
     1261 gtctttggca aaacctttcc agcggcccgg gtcatcgacg ctgaaataca cctgattacg
     1321 caggcggttc tgttcggctg attcttttgc aggttgcccg caggcggtgc acaccagcag
     1381 taaggtgaga attgagcccg aggggcgcat aggttcatcg gcaaattgtg aaggtgcgtg
     1441 caactacaaa caggctggcg acagttgctc cgcccaattg ggccagaagt tcagcagggt
     1501 tggccagagt ccagacgcga agcagccagg gtataccacc cctgaacagg gggtgggtgt
     1561 gacgaattca tcgttgtcaa aataagaaac cgcacgtctg agttcctacg cgtgctttca
     1621 ctaagaattt cagttctttc cttgttaact cttatcacga ttaactgcgc gcgcatcgac
     1681 gagctcggga gtcgcttttt taaaaaagaa ggcgcaataa cggcgagaat cactgcgaca
     1741 aaccttgccg gccgaaacct aagccttcag atcaaccagg acactttcac cacgtcggtc
     1801 gcgcagacgg tggtcagcac taacgtgcca cgcggcgaga atttcactat cacgattgcg
     1861 gcgcagccgg tgctgcccgc acaaacctgc acgctcacca gccccactac cggcgtcgcg
     1921 agcggtgact tgcttgaaat caccctcagc tgcacaacgc agaaattcac tatcagcggc
     1981 atgactacgg gttatgcggg cagcgggttg caggtgcagc tgaacggcgg tgaaattgtc
     2041 acggtgactg gttcgtcttt cagcttcacc acgctggtcg acgacaacag tacctacaat
     2101 gtgcagacaa agacgaaccc aactggcccg gcgcagatct gcacggcaca gaagaacgtg
     2161 gggctcgtta ccggtgcgaa tatcagtgac gtttcgatca attgttcgac ggtgacgtac
     2221 agcatcggcg gttttgtaaa cgggcttgcg gcggggcaat ccgtcacgct gcaggtgaac
     2281 ggcacacaga gtacggtcag gtcaacagac ggatttttca cgttcacgaa caagctcgcc
     2341 gaccaaacgg agtaccaaat cacagtgctg acccaaccgg ccggacagag ctgttcggtt
     2401 ggccgcggtg ccggcatact caatggtgaa ggtgcgtttt ccgcgcttat acagtgcagc
     2461 agcactacac acacgatttc gggaaatctg acgggtctga cgggtggttt tgtgaccctc
     2521 gtgtcgtcgt cgggtgaata cagaccgctc actgcgaacg gagcgtttgt ttttacacag
     2581 actttggcta ccggaaccga ctatgtcgtt gaggtcttcc gaaatcctgc tgcaccagcg
     2641 caggcctgca ctgtaaccaa tgcgacgggc acaatcagtg ccaatgttaa caacattaac
     2701 gtcagttgtg cttcaacagg ttatagcatc ggcggcaata gcactgtcag cggtttgact
     2761 tccgccgggc tggtgttgcg cctcaatgga tcgagtgaaa ccgtggtgcc ggcaaatgcc
     2821 acttctttca gtttcagcga gaccgttgca gctggcagca actacactgt gacaatagcg
     2881 gcgcaaccga ccgggcaaaa atgcacactc tcgaatgcga gcggcat
//
LOCUS       DAOQJF010000390         2909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_23322_length_2909_cov_4.563069, whole genome shotgun sequence.
ACCESSION   DAOQJF010000390 DAOQJF010000000
VERSION     DAOQJF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23322_length_2909_cov_4.563069"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLF85_15475"
     CDS             complement(<1..626)
                     /locus_tag="PLF85_15475"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21121.1"
                     /translation="MKKIVLALTALSLSGSAHAAVTTIYVHGFHFSIQKTETAKTCQG
                     QTTCTSYWGQQDNSSPVVHTGYDGRFNPTVAGAERGSTRLLENLNKYCRRDRGQSCRI
                     VNHSMGGLVSGFVLANYNRNNIYNVLYVTALVSAEGGSELANIGNPILQTLNVITLGL
                     SNFFLNFPDAVAVLKTSAARGAYDHNLTNGTPFYHLAGNTSEWYINWI"
     gene            808..1470
                     /locus_tag="PLF85_15480"
     CDS             808..1470
                     /locus_tag="PLF85_15480"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE21122.1"
                     /translation="MQTSLVPAKSTEIPKHWSSTFLSNKTLVEVHMAVHADKGFSTEF
                     TPRPGAEGPKAHAHGGFLATILDETMGSACWYHGLPVLAANLSVRYRRSVPLNGTYMC
                     EAKIERLETRRAMVAARIYKDDVTYCEATGVFVRVPIELLKQQPDMARMVEVVESIKS
                     GQSIEQLIELDRVRRAENQATTEASPAGDDWQALAERGQKLAGDILSLAGDVVKGLFR
                     RE"
     gene            complement(1623..1973)
                     /locus_tag="PLF85_15485"
     CDS             complement(1623..1973)
                     /locus_tag="PLF85_15485"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNE21123.1"
                     /translation="MRLNNLRILHQRRKELRKRLTPAEASLWNHLKNSQLEGMKFRRQ
                     HSVGRYILDFYCPAAKLAIELDGAVHNSAEAKLRDKERDAFLLSLEIKVLRFENKRVF
                     DDLDAVLAEIRAQL"
     gene            2304..>2909
                     /locus_tag="PLF85_15490"
     CDS             2304..>2909
                     /locus_tag="PLF85_15490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNE21124.1"
                     /translation="MSGLPFENSSAIKPGASAEELLHSVFGYEAFRLEQKQIIENVLA
                     KRDTLVIMPTGGGKSLCYQIPALIFEGLTVVVSPLISLMQDQVSQLNQLGVSAVCLNS
                     SLGLDEYEMNLLELRSGKVKLLYLAPETLFLERTQRLLGQVRVDCLTIDEAHCISDWG
                     HDFRPEYRKLLEIRRKLKGVVTIALTATATARVRDDIREQLA"
BASE COUNT          637 a          799 c          838 g          635 t
ORIGIN      
        1 aagatccagt tgatatacca ttcagaggtg ttgcccgcga ggtggtagaa cggcgtacca
       61 ttcgtcaggt tgtggtcgta ggcaccacgc gcagcgctgg tcttgagcac tgccactgca
      121 tcaggaaagt taaggaagaa attggagagc ccgagcgtga tcacgttgag cgtctgcaga
      181 atcgggttgc cgatgttcgc cagttcagag ccaccttcgg cagaaaccag tgcggtgaca
      241 tagagcacgt tgtagatatt gttgcggtta tagttcgcga gcacaaaacc cgaaacgaga
      301 ccccccatcg aatggttgac gatgcggcat gactggccgc ggtcacggcg acagtacttg
      361 ttcaggtttt cgagcagccg ggtcgaacca cgttctgcgc cggcaactgt cgggttaaaa
      421 cgcccgtcat agcctgtgtg cacgacaggg ctcgagttat cctgctggcc ccagtagctg
      481 gtgcaggttg tctgaccctg gcaggtcttc gctgtttctg ttttctggat cgagaaatgg
      541 aagccgtgca cgtagatggt agttacggct gcatgggcag agccactaag actgagcgca
      601 gtaagcgcta gtacgatttt cttcattcat gagtcctctt cacgctttcg ttagtaccta
      661 ctaaatgcaa gatcggtatg acaccgcgcc aaaaaacttc ggcaaataac aaaacgtctg
      721 tttactaaat actaaaccgc agaagttcac aggctcccat aaacctgatt ccgttttacc
      781 gccctgtagc attcgctaca atgcctcgtg caaaccagtt tagtacctgc caaatctacc
      841 gagataccca aacactggag ctccactttt ctcagcaaca agaccctcgt tgaagtgcac
      901 atggccgtgc acgcggacaa aggttttagt accgagttta ctccccggcc cggtgccgaa
      961 gggcccaaag cccatgcaca cggcggcttt ctcgcgacaa ttctcgatga gacaatgggg
     1021 agcgcgtgct ggtaccacgg cttgcccgtg ctggcggcga atctcagtgt gcgctaccgc
     1081 agatcggtac cgctgaatgg cacgtatatg tgtgaggcga aaatcgaacg tcttgaaaca
     1141 cggcgcgcga tggtcgctgc gcggatctat aaagatgacg tcacttactg cgaagcgacg
     1201 ggagttttcg tgcgcgtacc gattgagctc ttgaaacaac aacccgacat ggcgcgcatg
     1261 gtcgaagtcg tcgaatcgat aaagtcgggg cagagtatcg aacaactcat cgaactcgat
     1321 cgggtgcggc gcgcggaaaa tcaggctacg acagaagctt cacctgcagg cgatgattgg
     1381 caggcactgg cagagcgtgg gcagaagctg gctggcgaca ttctatcgct ggccggtgat
     1441 gttgtgaagg gattgtttcg aagggaatag ccgctgtcca gaccccctcc ccgctgcgcg
     1501 ggactccccc ttagcagggg gagagctgtt gtgacaatct tactcctccc ctgctaaggg
     1561 gaggtggctc gcgcagcgag acggaggggt atttcaggaa tgcccgaagg gcggggtggt
     1621 tcttacagct gcgcccgaat ctccgccagc acggcatcca aatcgtcaaa cacgcgctta
     1681 ttttcaaagc gcagtacctt gatctcgaga ctcagcagaa acgcatcgcg ttctttgtcg
     1741 cgcagttttg cctcggcact gttgtgtacg gcgccgtcga gttcgattgc gagctttgcc
     1801 gcaggacaat aaaaatcaag gatgtacctg cctacactat gctggcggcg aaatttcata
     1861 ccctcgagtt gtgaattctt gagatgattc cagagtgagg cctcggcggg tgtgagacgc
     1921 ttgcgcaatt ctttgcggcg ctgatgcagt atgcgcagat tgttcagccg cacggtgcga
     1981 gcattgcgtt gcagttattg tttcaagatg tttttcttgc ggaggggggg ataagaaccc
     2041 ctccgtcacc gaagacggtg ccacctcccc ttaacagggg aggagcaaga ttgctaactg
     2101 ggctctcccc ctgctaaggg ggagtggccg aaggccgcga cccgcttcgg gtctctgtct
     2161 tcagaggggg tttgtacgag gcatgcccca agggcggggt ggttcttgtg atgccgactc
     2221 gcgcagcgag acggaggggt atttcaggca agcagagaac ccctttccat tttacgcgcc
     2281 ctctcgacgc tgctggatgg cctgtgtcgg gtctgccttt tgaaaattct tcggccatta
     2341 aacccggcgc aagcgccgaa gaactactcc attccgtctt cggctatgaa gcgtttcgcc
     2401 tcgagcagaa gcagatcatc gagaacgtgc tcgcgaaacg cgacaccctc gtcatcatgc
     2461 ccacaggcgg gggaaaatcc ctctgttacc agatacccgc gctgatcttc gaagggctca
     2521 ccgttgttgt ttcgccgctg atctcgctga tgcaagacca ggtgagccaa ctgaaccagc
     2581 tcggcgtcag cgccgtctgc ctcaatagct cgcttggcct cgacgagtac gagatgaacc
     2641 tgctcgagct gcgctcggga aaagtgaaat tgctgtactt agccccggag acgctgtttc
     2701 tcgaacgcac acagcgcctg ctcggccagg tgcgcgtcga ctgcctcacc atcgacgaag
     2761 cgcactgtat ctctgactgg ggccatgact ttcgacccga gtaccgtaag ctgctcgaaa
     2821 tccgccgcaa actgaagggc gtggtcacca ttgcactgac cgcaacggcg actgcgcgtg
     2881 tgcgtgacga cattcgcgag cagctcgcg
//
LOCUS       DAOQJF010000391         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_23525_length_2894_cov_4.255372, whole genome shotgun sequence.
ACCESSION   DAOQJF010000391 DAOQJF010000000
VERSION     DAOQJF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23525_length_2894_cov_4.255372"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1652
                     /locus_tag="PLF85_15495"
     CDS             81..1652
                     /locus_tag="PLF85_15495"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE21125.1"
                     /translation="MDLPSLTIFFGGMLLLVLPFWPLQILRGRGFREPFLVYICLLWF
                     LSFFAGAIHFSAQVELGGHALKFRFSSAIYTLIFAGLVFVYIAEGINEARKVIAVSIA
                     SQIFLVLFQVFLYMARGYFLPASELALADKVLEPRAFNIGVSIVSTIVMLFLAVITFQ
                     FLVNQLRRAPLGLNMFLALWATMMLDSVIFLALTRPEAFSKSLAAHALFKTALLLLFV
                     FPLAFILRRFRRESELNLNRGTLDIFKKLENLEKDLERAHAELKSYAQNLEKMVEERT
                     TEIRAKSETMRRELELATEVQQAMLPGENSVGPFQVACLYRPCHEVSGDLYDYAELPD
                     GRIFVFIADISGHGVPSALVGAMCKMSLGGQDLARSNAGEILKKLSEEMKKVTTSHYL
                     TGTVLLIDPRARTIEYANGGHIPCLLQSASSAFLPLEATGTVIGSFISAPYDFRKIAY
                     PAGTRLILLTDGIVEQKNAAREEFGQERFQKALTETRSQNPAVALGEIWRRSSEFAGT
                     ERFSDDVTLLIADLS"
     gene            1652..>2894
                     /locus_tag="PLF85_15500"
     CDS             1652..>2894
                     /locus_tag="PLF85_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE21126.1"
                     /translation="MLQRFSLRLAGAWTMPVLHTAATVYLLSVVVYRVVLVFALLFAE
                     AAPLGAQKGVIPVLAVLAGLGHDLFLLVLIYALVILFRLARPGAGMGRAARNLVLLLL
                     SLALFWNAMILGSHTNLMFSMNTGFTWSIFVEFFTVLSLREFISLMTIKDYIAVVFPP
                     ALFLLLVLRRPVHGVPLRKAATLLVSVFGLQLLSSLPLAGRVPDELSMNPHAFFARDA
                     ARQLLAARETRPLRTGNHLNVALNQPPFVTGVKEKTPAPVVRSNARANVVVIILESTA
                     SEYIFDTTKYAGGKMPMPYLYSLAEKSLWFTRHFASNNSSPRSIFSIFSGLYESPETR
                     FFSMEKKLRVPHLLDHLGKDYRAFLYTPADLDWYFPKAWFRNRGFNELNDYHRLKQVP
                     EYKAGPTSVRDEFKTVQEFTRE"
BASE COUNT          548 a          818 c          804 g          724 t
ORIGIN      
        1 caagcagaat gggggtttgg cgcggtaaga tgccgggctt ttgtactttg cgccgccata
       61 ggattctttt tttgagcggc atggacctgc catcgcttac catttttttc ggcgggatgc
      121 tgctgctggt actgccgttt tggccgctgc agattctgcg cggccggggg tttcgtgaac
      181 cctttcttgt ctatatctgc ctgctctggt tcttgtcctt ttttgccggg gcaatccatt
      241 tttctgcgca ggtggagctg ggcggccacg cgctgaagtt ccgcttttcc tcagcgatct
      301 acacgctgat ctttgcgggt cttgtttttg tctatatcgc cgaaggcatc aacgaagcgc
      361 gcaaagtgat tgcggtgtcc attgcctcgc agattttcct ggtcctgttc caggtattcc
      421 tgtacatggc gcgcggttac tttctgcccg cctcagaact ggcgcttgcc gacaaggtac
      481 tcgaaccgcg tgcttttaac attggcgttt caatcgtcag caccattgtg atgctttttc
      541 tcgcggtcat cactttccag ttcttagtga accagctgcg gcgcgcaccg ctgggactca
      601 acatgttcct ggcgctttgg gcgacaatga tgctcgattc ggtgatcttt ttggcgctga
      661 cccgccccga ggccttcagc aagagcctgg cagcgcacgc gcttttcaaa accgccctgc
      721 tgctgctctt tgtattcccg ctggcattca ttctcaggcg gttccggcgc gagtcggagc
      781 tcaacctcaa ccggggcaca ctcgacatat tcaagaaatt agagaatctt gaaaaagacc
      841 tggagcgcgc gcatgccgaa ctcaagagtt atgcgcagaa tctggaaaag atggtagaag
      901 aacgcacaac cgagattcgc gcgaaatccg agacgatgcg acgtgagctc gaactcgcga
      961 ccgaggtgca gcaggcgatg ctgcccgggg aaaactcggt cgggccattc caggtcgcct
     1021 gcctctaccg tccctgccac gaggtgtcag gcgatctcta tgactatgcc gaactgcctg
     1081 acggccgtat tttcgtcttt attgccgaca tttcagggca cggtgtgccg agtgctctgg
     1141 tgggggcaat gtgcaagatg tctcttggcg ggcaggacct ggcgcgcagc aatgctgggg
     1201 aaattctgaa gaaactttcc gaagagatga agaaggtaac gacgagccac tacctgaccg
     1261 ggaccgtgct cctcattgac ccgcgcgcgc gtactatcga gtatgcgaat ggcgggcata
     1321 tcccctgcct gctgcagagc gccagctcgg cctttctgcc gctcgaagct acaggtacag
     1381 tgatcggttc attcatcagt gcaccgtacg atttccgcaa gattgcgtac ccggctggta
     1441 ccaggctcat actactgacc gacggcatcg tagaacaaaa gaatgccgct cgcgaagaat
     1501 ttgggcagga gaggtttcaa aaagccctca ccgaaacccg ttcgcaaaac ccggccgtgg
     1561 cactgggcga aatctggcgg cgttcgagcg aatttgccgg tactgagcgc ttctcagatg
     1621 acgtcacgtt gctgatcgcc gatctttcct gatgctgcag cgtttttctc tgaggcttgc
     1681 aggtgcctgg acgatgccgg tactacatac agccgcaact gtctacctgc tttcggtagt
     1741 cgtctaccgc gtggttctgg tatttgcgct gctctttgct gaagcggcac cgctcggtgc
     1801 gcaaaaggga gtgattccgg tcctggctgt tcttgccggt cttggccatg acctctttct
     1861 tctggtgctc atctacgctc tggtaatttt atttcggttg gctcgccccg gggcgggcat
     1921 ggggcgggcg gcgcgcaacc tggtacttct gctgctcagc ctcgcgctct tctggaatgc
     1981 tatgattctg gggtcgcaca cgaacctgat gtttagcatg aacaccggtt tcacctggag
     2041 catattcgtc gaatttttca cagtcctcag cctgcgcgaa tttatatcgc tgatgaccat
     2101 caaagattat atcgcagtgg tttttccacc cgcgctcttt ttgcttttgg ttctgcggcg
     2161 gccagtgcat ggcgtgccat tgcgcaaggc cgcaacgcta ctggtctcgg tctttgggct
     2221 tcagctactt tcttcgctgc ccttggcggg tagggtaccc gatgagttga gcatgaaccc
     2281 gcatgcgttc tttgcccgcg atgcggcgcg ccaacttctg gcggcgcgtg aaacgcggcc
     2341 gctgcgtacc ggtaaccatt tgaacgtcgc gttgaaccag ccgccgtttg ttacaggcgt
     2401 taaagagaaa actcccgcgc cggtcgtgcg cagcaatgcc cgggccaatg tggtcgtgat
     2461 cattctcgaa agtactgcct cggagtatat cttcgatacg accaaatacg cgggcggcaa
     2521 gatgcccatg ccgtacctgt attcgctggc agagaagtcg ctctggttta cccggcattt
     2581 tgcctcgaac aacagctcgc cgcgttcgat attttccatc ttttcggggc tgtacgagag
     2641 tcccgaaacg cgcttttttt cgatggaaaa aaaactccgc gtaccgcacc tgctcgacca
     2701 cttaggcaaa gactaccggg catttctcta tacaccggcc gacctggact ggtatttccc
     2761 gaaagcctgg tttcgcaacc gcggcttcaa cgagttgaat gattaccacc ggctgaagca
     2821 ggtacctgaa tacaaagcgg ggcccacctc ggtacgcgac gagttcaaaa ccgtgcagga
     2881 gttcacccgc gaat
//
LOCUS       DAOQJF010000392         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_23639_length_2885_cov_4.692933, whole genome shotgun sequence.
ACCESSION   DAOQJF010000392 DAOQJF010000000
VERSION     DAOQJF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23639_length_2885_cov_4.692933"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PLF85_15505"
     CDS             <1..1340
                     /locus_tag="PLF85_15505"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HNE21127.1"
                     /translation="VPNPEWDWSRIDTSKVSFPDKFLWGAATSAYQIEGGLYNNNWYL
                     WERTPQRFGVPRIARDEKAGLAANHYELYKEDVKLMADFGLNAYRFSIEWSRIEPREG
                     EFDLKAIAHYRDLIRRLKAAKIEPMITLHHFTDPIWFVDKGGWEKEQNLAYWMRYAKR
                     MFQEFQGDVNIWSTFNEFNLVPVSGWLDGGFPPGKTDFQLFATVSKHMLMGHVQTYLE
                     FKKLSRTKKHQVGAILAILEARPYNRWFILDWLAAYYEERIWMQAMIEFFATGKYNVK
                     LPFRDSFTYTDTDGVRAMDFFGINYYTRTAVVFNIFSPTLFHRIQLPGFPTTDMGWAV
                     YPEGLYFAVKRYSAIGVPIIITESGLADANDTIRTTFIQQHLYVLSQILKEGYDVRGF
                     YYWSLLDNFEWLEGYDKRFGLYAVDYKNFKRTLREGSKEYQKIIRRFPGRGGS"
     gene            1343..2479
                     /locus_tag="PLF85_15510"
     CDS             1343..2479
                     /locus_tag="PLF85_15510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE21128.1"
                     /translation="MRTLPIYLVFLVMGFGDLIGPLVSLVKENLQTTQFEASFIAFAG
                     FIMFGLLSIPVGVLQANLGKKPVAFGGLILVLAGATTPLLGLTSYAGYLAAVLLLGAG
                     VTVLQVAGGPLLQEASPPGKFAQNVTFGQFVKGIGTLSAPLIPILAYRYFDNRWQLVF
                     PIFGSATLIAMLALFFHNSKDESKNAATVAACVALLANRRVALLVLAIFLYVGAEVCI
                     SSMLPTILEKNYGFRLADYGMAGTGLFFLSLMTGRFAGALLLTKIRPAAFLLLCCGLA
                     LAGLALLATATGYLGFAAAMLIGFGFANIFPLVYAIGMDAYPTRASELSGLMVSAIAG
                     GAILPPVMGYVSEHSSLVAALVVPTLCIIYIALLALTERRQHAA"
     gene            2469..>2885
                     /locus_tag="PLF85_15515"
     CDS             2469..>2885
                     /locus_tag="PLF85_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE21129.1"
                     /translation="MPHKIHIAPPFEKDERIVLTLDAGGTKFSFSAMAGGKQVVHPIE
                     FPSHGDDLDKCLKTLVGGFAEIQSRLHSKPAAISFAFPGPAWYKEGIIGDLGNLPAFR
                     GGVPLGPMLAKQFGVPVFINNDGDLFTLGEAVGGFLP"
BASE COUNT          564 a          869 c          800 g          652 t
ORIGIN      
        1 ctgtgcccaa tcccgagtgg gactggtcgc gtatcgacac gagcaaggtt tcgtttcccg
       61 ataaattcct ctggggcgct gcgacctccg cctaccagat tgaaggtggg ctctacaaca
      121 ataactggta cctgtgggag cgcacgccgc agcgcttcgg tgtgccacgc atcgcgcgcg
      181 acgaaaaggc gggcctcgct gcaaaccact atgaactcta caaagaagac gtgaagctca
      241 tggcggattt cgggctcaac gcctaccgct tttccattga gtggagccgc atcgaaccgc
      301 gcgagggtga gtttgacctc aaggcgattg cacactatcg cgacctgatc cggcggctca
      361 aggccgcgaa gatcgagccg atgatcacgc tgcaccactt caccgacccg atctggttcg
      421 tcgataaagg tggctgggaa aaagagcaga acctggccta ctggatgcgc tatgccaagc
      481 gcatgttcca ggaattccag ggtgatgtga atatctggag cactttcaac gagtttaacc
      541 ttgtgccggt ctcaggctgg ctcgatggtg gatttccccc cggcaagaca gactttcagc
      601 tgtttgcgac tgtctcgaag cacatgctga tgggacacgt gcagacctac ctcgagttca
      661 aaaaactctc gcgcacgaaa aaacaccagg tcggcgcgat tctggcaata ctcgaagcac
      721 ggccgtacaa ccgctggttc atactcgact ggctcgctgc ctactacgaa gaacgtatct
      781 ggatgcaggc aatgattgaa ttttttgcca cgggtaagta taacgtcaag ctgccgttcc
      841 gcgattcgtt tacctacacc gacaccgacg gcgtgcgcgc gatggatttt ttcggtatca
      901 attactacac acgcacggca gtagttttca acattttctc cccgacgctc tttcatcgca
      961 tccagctgcc tgggtttccc actaccgaca tgggctgggc cgtctacccc gagggcctct
     1021 acttcgccgt gaaacgttac tccgcgatcg gcgtgccgat tatcatcacc gagtcggggc
     1081 tcgccgacgc caacgacacg attcgcacga cgttcatcca gcagcacctc tacgtgctct
     1141 cacagattct gaaagaaggt tatgacgtgc gcggcttcta ttactggtcg ctgctcgaca
     1201 acttcgagtg gctcgaaggt tatgacaaac gtttcggtct ctacgcggtc gactacaaga
     1261 acttcaagcg caccctgcgc gaaggttcga aagaatacca gaaaatcatc cgccggtttc
     1321 ccggccgggg aggcagttga gcatgcgcac gctaccgatt tacctcgttt ttctcgtgat
     1381 gggcttcggc gacctgattg ggccactggt ctccctggtt aaggaaaacc tgcagacgac
     1441 acagttcgag gcatcgttca ttgcgttcgc gggctttatc atgttcggtc tgctatcgat
     1501 tcccgtgggg gtgctgcagg ccaatttggg caaaaagccg gtcgccttcg gcggattgat
     1561 tcttgtactg gccggcgcca caaccccgct gctcggtctc acgagttacg caggatatct
     1621 cgccgcagtg ctgctgctcg gcgccggagt caccgtgcta caggttgccg gcggtccgct
     1681 gctgcaagag gcttcaccac ccgggaagtt tgcacagaac gtgacctttg gccagttcgt
     1741 gaaaggtatc ggtacgctca gcgctcccct catacccatt ctggcctacc gctatttcga
     1801 caaccgctgg cagctggtgt tcccgatctt cggctcagct acgctcattg ccatgctcgc
     1861 cctgtttttc cataacagca aagatgagag caagaatgcc gcaaccgtcg ccgcctgcgt
     1921 cgcgctgctg gcgaaccgcc gcgtcgcact gctggtgctc gcgatctttc tgtatgtcgg
     1981 cgccgaggtc tgcatcagct cgatgctgcc gacgatcctg gaaaaaaact atggctttcg
     2041 cctcgctgac tatggcatgg cgggcacggg cctctttttt ctttcgctga tgaccggccg
     2101 ctttgccggc gcgctgctgc tcaccaaaat tcgcccggcg gcatttctgc tgctctgctg
     2161 tggccttgcg ctcgcaggcc tcgcactgct cgccacggca acgggttacc tcggctttgc
     2221 tgcagcgatg ctcatcggtt ttggtttcgc aaatattttt ccgctggtct acgccatcgg
     2281 catggacgca tatccaacgc gtgcgagtga actctctggc ctgatggtga gcgcgatcgc
     2341 ggggggagct atactgccac cggtcatggg ctacgtgagc gagcatagct ccctggttgc
     2401 ggcactggtt gtgccgaccc tgtgtatcat ctacatcgcg ctgctcgcgc tgaccgaaag
     2461 aaggcagcat gccgcataag attcacatcg ctccaccgtt cgagaaagac gagcgcatcg
     2521 tgctgacact cgatgcgggc ggtacgaagt tttcgttctc ggcgatggcg ggcggcaaac
     2581 aggttgtcca cccgatcgag tttccctcgc acggcgacga tttggacaaa tgcctgaaga
     2641 ccctcgtcgg cgggttcgcc gagatacaga gccgcctgca ctctaaaccc gcagcgatca
     2701 gctttgcttt tcccggcccg gcgtggtata aagaaggaat catcggcgac ctcggcaacc
     2761 tgcccgcgtt tagaggggga gttccccttg gcccgatgct cgccaagcag ttcggcgtgc
     2821 ccgtcttcat caacaatgac ggcgacctct ttacgctcgg cgaagccgtc ggtggatttc
     2881 tgcct
//
LOCUS       DAOQJF010000393         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_23923_length_2865_cov_4.214591, whole genome shotgun sequence.
ACCESSION   DAOQJF010000393 DAOQJF010000000
VERSION     DAOQJF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23923_length_2865_cov_4.214591"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1612)
                     /locus_tag="PLF85_15520"
     CDS             complement(170..1612)
                     /locus_tag="PLF85_15520"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNE21130.1"
                     /translation="MSNTKYNSLPGAKPHPAYGTPPLDKGRGRGWGLFLLLATLLLNA
                     CVAQDDKEVLFSDTELAIIKTLIYKAPDGEASNQYVNNSAAATLGQKFFWDPQFSGDI
                     VVQGNRGASFAPQTTNGSARQISCATCHAPSFGWADNTSIPNNVSLGANLTNRNSPTV
                     LNTSFNTYSTWTGGTDSVWAVMRPAIEGPTTQNFSRVGVASIICNNATYTANYTTAFG
                     APDCTGVPAMTTIAGQTDNWGRNDFASLAAAVQDRVNLLFANFGKAIGAYEHLLVSKN
                     SAFDQWAEGNENAMSASQKRGLKIFIGKGNCVRCHSGPNFSDGSFHNLGVPQIGGALG
                     VLDDGRYTGISDLLTGSNSTFNTSSGFNATPSVNRVSGLARTDADIGKFKTPTLRSVN
                     KTPPYFHNGTFNSLWDVVNFYNFAGNAGNFPGTKDPVLTTRRMTNEEMEDLVNFLKAL
                     EGEALPTSLTTSPGGFGTTSGWCTPPVVCP"
     gene            complement(1625..>2865)
                     /locus_tag="PLF85_15525"
     CDS             complement(1625..>2865)
                     /locus_tag="PLF85_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21131.1"
                     /translation="IVPGSGSGVVAEGEAPGAAKGAPTLLITGFVDMGFAFAQNKGVG
                     FQKDVGNNFQQFADKTAVFVGDPASTYMNTRHEAHDLGEAGNFPLAFDGMQGKGNPMG
                     ALNSATIDMIAEVHENVRLQVSTEFLPRQPVVQNQTGMGSFVNLDMAFLDWKPLSQYD
                     ITVTAGKFIGITGIEYRWRRAPDRWTVTPTLVGRYMDGNPLGVKVRGKHFDKRFVWNI
                     GVTNGNSFTQDTRIFENVESNFGKTYAGRLSYDFAFGPVTLLELGISGSYGSQDMQAS
                     DKPKQWDWGPDFQIAWKALQLRGQWFKVNTDGGGINETKNYIAEAWYVEGMYSFPGLF
                     DLVNTFGIFTRFSRRVATEIDPKYVFIVDIAQITPGVRVDITENIIFKAEYSINLEVG
                     YIKNYAFDNNIFTSSLVAKF"
BASE COUNT          643 a          774 c          779 g          669 t
ORIGIN      
        1 ttctagtagc tatgggggct ctactagcta tgggcttaag cccatagcta gtagaagaaa
       61 cgccctaaca cccccacgag ggagataatc gatgagaaaa ctcttctaga atcttttagc
      121 tatggggtta accccatagc taaaagttga tcacaacacc aaatcttaac tacggacaaa
      181 cgacaggcgg cgtgcaccag cccgacgtgg tgccaaaacc gccaggtgaa gtagtaaggc
      241 ttgtcggaag agcctcaccc tcaagggcct tcaagaaatt caccaaatct tccatctcct
      301 cattcgtcat acgccgcgtg gtgagaacgg ggtctttggt gccgggaaaa ttgcctgcat
      361 tccccgcgaa gttgtaaaaa ttcactacat cccacaggct gttgaaagtg ccgttgtgaa
      421 aatacggcgg ggtcttgttg accgaccgca gcgtcggtgt tttgaatttg ccgatgtctg
      481 catcagttct ggcgaggcca gacactctgt taaccgaggg tgtcgcatta aagccagaac
      541 tcgtattaaa agtactgttt gagccagtca gcagatcgct aatgcctgta tagcgcccat
      601 catcaagcac acccaacgct ccaccaatct gcggcacgcc gaggttgtgg aatgacccat
      661 cgctgaaatt agggccgcta tgacaccgca cgcagtttcc cttgccgata aagattttca
      721 ggccgcgctt ttgtgatgca ctcatagcat tttcattgcc ttctgcccat tggtcgaagg
      781 cggaattctt ggaaaccaac agatgctcat aagccccgat ggctttgccg aagtttgcga
      841 agagtaaatt tactcgatct tgcacggcag ctgcaagaga tgcgaaatca ttacggcccc
      901 aattatccgt ctgacccgcg atcgttgtca ttgcaggtac acctgtgcaa tcaggcgccc
      961 cgaaggcggt tgtataattg gctgtatagg ttgcgttgtt gcagatgata gaggcaacgc
     1021 ctactcgact gaaattctgt gttgtaggac cctcaattgc cggccgcata actgcccaaa
     1081 ctgaatcggt accacctgtc caggtgctat aagtattgaa tgaagtattg agaaccgtag
     1141 gtgaatttct attcgtcaag ttcgcaccta gtgacacgtt gttaggtatc gaggtattgt
     1201 cagcccaacc aaaactcggc gcgtggcagg tcgcacagct tatttgccgc gcagagccgt
     1261 tggtagtctg cggtgcgaaa cttgcacctc gattaccttg aacaactata tcccccgaaa
     1321 actggggatc ccagaagaac ttctgcccca gtgtagctgc ggctgagttg ttgacgtact
     1381 ggttgctcgc ctcgccgtct ggtgccttgt agatcaacgt cttgataatc gccagctcgg
     1441 tatcagaaaa caggacttct ttatcatcct gcgcgacgca ggcgttgaga agaagggtgg
     1501 cgagcaggag aaatagaccc cacccccgtc ccctcccctt atcaagggga ggggtgccgt
     1561 aggcggggtg gggttttgca cctggaaggg aattgtattt cgtattgctc ataactcccc
     1621 ctgcctaaaa tttcgccacc agcgatgacg tgaagatgtt gttgtcgaat gcatagttct
     1681 tgatgtagcc gacttcgagg ttgatcgaat actcggcctt gaagatgatg ttctcggtga
     1741 tgtcgacgcg cacgccgggc gtgatctgtg cgatgtcgac gatgaacacg tacttggggt
     1801 cgatttcggt cgcgacgcgg cggctgaaac gcgtgaagat gccgaacgtg ttcacgaggt
     1861 caaagagccc cggaaacgag tacatgccct cgacatacca ggcttcggcg atgtagttct
     1921 tggtttcgtt gatgccaccg ccgtcggtgt tgaccttgaa ccactgcccg cgcagctgca
     1981 gcgccttcca cgcgatctgg aaatcggggc cccagtccca ctgcttgggt ttgtcagagg
     2041 cctgcatgtc ctgcgagcca tacgaacccg aaataccgag ttcgagcagg gtcaccggcc
     2101 cgaacgcgaa gtcatagctg aggcggccgg cataagtttt gccaaagttg ctttcgacat
     2161 tctcgaagat gcgcgtatct tgcgtgaagg agtttccatt ggtcacgcca atgttccaga
     2221 cgaaacgttt gtcgaagtgc ttaccgcgca cttttacccc gagtgggttg ccgtccatgt
     2281 aacggccgac gagcgtcggt gtgaccgtcc agcggtcggg cgcacggcgc caccggtatt
     2341 caataccggt aatgccgatg aacttaccgg cagtgactgt gatgtcgtac tgcgacagtg
     2401 gcttccagtc gaggaacgcc atgtccaggt tcacaaagct gcccatgccg gtctggttct
     2461 gtacgaccgg ctggcgcggc agaaattccg tcgaaacctg caggcgcaca ttttcatgca
     2521 cctcggcaat catgtcgatc gtcgcgctgt tgagcgcgcc catcgggttg ccctttccct
     2581 gcatgccgtc gaacgcgagc gggaagttgc ccgcttcgcc gaggtcgtgc gcttcgtggc
     2641 gagtattcat gtatgtagat gcggggtcac cgacgaagac cgcagtcttg tcggcaaact
     2701 gctggaagtt gttgccgacg tctttttgaa atcccacacc cttattctgc gcaaaggcaa
     2761 aacccatgtc gacaaagccc gtgatcagca gggtgggagc tcccttggct gcgccggggg
     2821 cttcaccctc ggcgacaaca cccgaaccgc taccggggac tattt
//
LOCUS       DAOQJF010000394         2845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_24189_length_2845_cov_5.708961, whole genome shotgun sequence.
ACCESSION   DAOQJF010000394 DAOQJF010000000
VERSION     DAOQJF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24189_length_2845_cov_5.708961"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1294
                     /locus_tag="PLF85_15530"
     CDS             344..1294
                     /locus_tag="PLF85_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21132.1"
                     /translation="MPALALTGNIKQSVITLYAEATNTVSPQAAVFTTTGKKVLVGGV
                     EQVSGVTLNDFTSPVTYTVLAEDGTTFEYLVMVLAPRLVPGLSAWYKADAQSYGDGVT
                     MTTWTDSSGNGNNLDTSLGGGLPFFKVGIINGKPVGRFATGSFSGIQRTSLSGFSGNS
                     VTTFIVFRRTAYTGQEQYFYNLGPGSCNGLTQSLQATFGPILAQIPCGPYFVNAVPDF
                     ADTASFHLLVFRYDYSTNNSTQYLDGAVQFAGAVSGGPYTLPTPSKLIVGNFDSGTRG
                     LDADLAEFLYFSGALSDTEVRRLSCYLSEKYALPVASACP"
     gene            1472..1759
                     /locus_tag="PLF85_15535"
     CDS             1472..1759
                     /locus_tag="PLF85_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNE21133.1"
                     /translation="MAIKPLADRVLIEAAPEKEEKIGSIFIPDTAKEKPNVGIVVAAG
                     PGKVTDDGKTVPLQVKAGQKVLYGKYSGTEIKENGKEYLIVRESDILAIVE"
     gene            1784..>2845
                     /gene="groEL"
                     /locus_tag="PLF85_15540"
     CDS             1784..>2845
                     /gene="groEL"
                     /locus_tag="PLF85_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNE21134.1"
                     /translation="MAKILEYDEVARAKLMAGINKLANAVKVTLGPRGRNVVIDKKFG
                     APTITKDGVTVAKEIELEDPYENMGAQLVKEVSTKTNDVAGDGTTTATILAQAIAREG
                     IKNVTAGANPMDLKRGIDKAVEAVVEYIGKIAKKVESKDIAAVATISANNDAEIGKLI
                     ADAMDKVGKDGVITVEEAKSIETTLDTVEGMQFDRGYVSSYMVTDPENMTATLENAYV
                     LIYDKKISAVKDVAPILNQVAQAGKPLLIIAEDLEGEALATIVYNTIRKAINCVAVKA
                     PGFGDRRKAMLEDIAILTGGQVISEELGQKLESTTIQMLGKVEKVIIEKENTTIIKGG
                     GKDNEIQHRIKQIKKQIEET"
BASE COUNT          757 a          710 c          748 g          630 t
ORIGIN      
        1 ctggttgagt ggctatggtg attactccgc tgcgcgcaaa gagatctata cgtcgttgcg
       61 cctgacgtac aagatcgcgc cgtttggttt tgacgatgaa gtaccggtgg gtgatcacgt
      121 ggcttatcgc cgttgagtac gctatgcttg atgcaagctc aatccctcaa gcggattgcc
      181 aatttatcga agagcgcaac tttgcccggc agcaaaatcg actatgcggc gcgttggcat
      241 attcatccta ttaactctaa agctctcctg cacagggtgg ggtgatttct gggatcgcag
      301 gcccaagact caggctagcc ccaaagcgat cacatccttt tcattgcctg cactcgcact
      361 cactggcaac attaaacaat cggtgattac gctctacgcc gaagcgacga atacagtctc
      421 accgcaggcg gcagtattta cgacgaccgg caagaaggtt ctggtcggcg gggtagaaca
      481 ggtcagcggc gtgactctca atgatttcac cagccctgtc acgtacactg ttctagctga
      541 agatggtaca acctttgaat acctggtgat ggtgcttgct ccccgcctgg ttccgggact
      601 gtctgcctgg tacaaggcag acgcgcagtc ttacggagat ggtgtgacaa tgacgacctg
      661 gacagattcg agcggcaacg gtaataatct cgatacttcg ttgggtggag gcctgccgtt
      721 ctttaaagtt ggaattatca acggcaaacc cgtggggcgg tttgccacag gtagcttcag
      781 cggcatacag cgtacttcgc tttcaggttt ttctggaaac tctgtgacta cgttcattgt
      841 ctttcgcaga acggcctata ctggtcagga gcagtatttc tataacctgg ggcccggcag
      901 ctgcaatggt ctgacgcaat cgctgcaggc aacctttggg ccgatcctgg ctcagatacc
      961 gtgtgggccc tatttcgtga atgctgtccc ggattttgct gataccgcca gctttcacct
     1021 tctggttttt cgctatgatt actcgaccaa taattcgacg caatatcttg atggcgcagt
     1081 ccagttcgcc ggggcagttt caggcggccc ctacaccctt ccaacccctt caaagctcat
     1141 cgtcggcaat ttcgattccg gtacccgcgg gctggatgcc gatttagcgg agttcctcta
     1201 tttcagtggc gccctcagcg acacagaagt tcgacgcctg tcctgttacc tcagcgaaaa
     1261 atacgcactc ccggtcgcat cagcatgtcc gtagtctgaa tctcaaattt tttttgccgg
     1321 cctgtccgtt tttaataaat tggcactaag gcgtcgagag tgctaacaca ccttgtgggg
     1381 agtgatagca tattgagatg cgagtgctca aataactcct gtttccaagg gaggtgtttg
     1441 ggccccattt ggtccaggag gatactagaa tatggcaatt aaaccattgg cagaccgcgt
     1501 tcttatcgaa gcggcacctg aaaaagaaga aaaaatcggc agcatcttta ttcccgatac
     1561 ggcaaaagaa aagccgaatg tgggtatcgt agttgcggca ggcccaggca aagtaacaga
     1621 cgacggaaaa actgttccgc tgcaggttaa ggccggacag aaagtgctct acggaaaata
     1681 cagcggcact gaaatcaaag aaaacggcaa agagtacctc atcgtgcgcg aatcagatat
     1741 tctcgcgatc gttgaataaa gcgaatattt ttaaggagat aacatggcaa aaatactgga
     1801 atatgatgaa gtagcccgtg caaaactgat ggcgggtatc aacaagctcg cgaatgcagt
     1861 aaaggtaacc ctcgggccac gcggccgtaa tgtggtcatc gacaagaaat tcggagctcc
     1921 cacaattact aaggacggtg ttaccgttgc gaaagaaatc gaactcgaag acccgtacga
     1981 aaacatgggc gcgcagctcg tgaaagaagt ttctacaaaa actaacgacg tagccggcga
     2041 cggtacgacc accgcaacga ttctcgcgca ggcaattgca cgcgaaggta tcaagaacgt
     2101 taccgcgggc gcaaacccaa tggacctcaa gcgcggtatc gacaaggctg ttgaggctgt
     2161 tgttgagtac attggcaaga tcgccaagaa agttgaaagc aaagacatcg ccgctgtggc
     2221 gaccatctct gcaaacaacg atgctgagat cggcaagctg atcgctgatg caatggacaa
     2281 agtcggtaaa gacggagtga tcactgtgga agaggcgaag tcgatcgaga caaccctcga
     2341 cactgttgaa ggtatgcagt tcgaccgcgg ttatgtttca tcttacatgg tgactgaccc
     2401 ggaaaacatg actgcaactc tcgagaacgc ttacgtcctg atctacgaca agaagatcag
     2461 cgctgtaaaa gacgttgctc caattctgaa ccaggttgca caggctggca agccactgct
     2521 tatcatcgct gaagatctcg aaggcgaagc gctggcgacg attgtctaca acactatccg
     2581 taaggcaatc aactgcgtgg cagtaaaagc tcctggcttc ggcgaccgcc gcaaggcaat
     2641 gctcgaagac atcgcgattc tgaccggtgg acaggtaatc tctgaagaac tcggccagaa
     2701 actggaaagc acaactatcc agatgctcgg taaagttgag aaggtgatca tcgagaaaga
     2761 aaacacgacg atcatcaagg gcggcggcaa agacaacgaa atacagcacc gcatcaagca
     2821 gatcaagaag cagatcgaag agacc
//
LOCUS       DAOQJF010000395         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_24252_length_2840_cov_5.277199, whole genome shotgun sequence.
ACCESSION   DAOQJF010000395 DAOQJF010000000
VERSION     DAOQJF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24252_length_2840_cov_5.277199"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLF85_15545"
     CDS             <1..795
                     /locus_tag="PLF85_15545"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE21135.1"
                     /translation="HRPFHLVNLSFPRTATTSLAGIFQRHGATHEFMRVETINALLNY
                     RDGKQTAAAVRQLLLERARLSGKPVDSAAFLFLSPEIVIETFPKAKFVFVVRDCESWI
                     VSLLDMEMRVFDSIRRGQNSIDFSYHPRYARVLSPHFSEQMLLDEASLKRAIPVLIPE
                     LAHFWRSQSIVVFESMKAVAPANRLIIRLVDFSNAFSNFAHLAGVPENSLVRQNVHLN
                     RDAFPEQIQKLIGPSFLSNICSKEQRTLDNYLNRNPGLLNVPLPGK"
     gene            795..1349
                     /locus_tag="PLF85_15550"
     CDS             795..1349
                     /locus_tag="PLF85_15550"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNE21136.1"
                     /translation="MNDLTHWQIFSRFARIGFTSLGASTTEIMRQHLVDEKQWLTTDE
                     FRENLSLAAIIPGPFHVNLACFSGYRILGFTGCIAAVVGYVIPGFVILALIIASVGSE
                     RVSQLVQKNPAFINGVTAGICGLLMHSILRLSDRTLEMRSDWLLILILALLMQTMQIP
                     FLLLALLAGLYFLGRSLYFRSRIR"
     gene            1346..1897
                     /locus_tag="PLF85_15555"
     CDS             1346..1897
                     /locus_tag="PLF85_15555"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNE21137.1"
                     /translation="MTSFLQSLLDSLLISVQSFGGIHSYWALIERQFVTGCTEHIYPE
                     LGEICRPMFKASLALAEVIPGPQVNAAAWLFYHSSGVTAVFGILLGALLPGITLAPLL
                     VTLLRHARLQNLLREFIAGASVAAIALLLVFLLRLIPHTTAFYQQVTFGLIGAACFVV
                     RHYTKANVLVIMLAAGVAGWLFL"
     gene            complement(1909..2628)
                     /locus_tag="PLF85_15560"
     CDS             complement(1909..2628)
                     /locus_tag="PLF85_15560"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNE21138.1"
                     /translation="MKFDRNDIFTGLFVLVGSVFGLSVLLFIAGYNLLDDRKEYWVRF
                     EKLAGVKKGTQIKIKNFTIGEVQEVLPIYGTDLHFKARVLVDKEFTIYQGTTVNITNQ
                     NVIGDAILDLTPSLVKKYPLKPHDTLFATNVTNLDQMVSQISNLVSTVNKMVETFGAL
                     AGDNKTDVKMLLTNLNGSITRVNGLLDASQGEIVAIMRNIRSTSTTLDKFTKEMAANP
                     WKILDKKGGPGATTGDSAALP"
     gene            complement(2625..>2840)
                     /locus_tag="PLF85_15565"
     CDS             complement(2625..>2840)
                     /locus_tag="PLF85_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21139.1"
                     /translation="EDIRLRNITYILSSYHPTIFDGRDLKFMLLYRGEVVFQGNLADI
                     KRADNVFLEQYRAYRTQGPMPPFNATL"
BASE COUNT          623 a          758 c          715 g          744 t
ORIGIN      
        1 cacaggccgt ttcacctggt caacctgagc tttccaagga ctgcgacaac ttccctggca
       61 ggcattttcc agaggcatgg agcaacccac gaattcatgc gggttgaaac catcaacgca
      121 ctactcaatt accgcgacgg taagcagact gctgccgcgg tgcgccagct tcttcttgag
      181 cgggccaggc tcagcggtaa gccagttgat tcggcggcgt ttctttttct ttccccggag
      241 atagtcattg aaacctttcc gaaagcaaag ttcgtttttg tggtgcggga ctgtgaatcc
      301 tggatagttt ctctgctcga catggagatg cgtgtattcg attccattcg cagagggcag
      361 aacagtattg acttcagcta ccacccacgg tacgcccggg tactttcacc gcatttttcc
      421 gagcagatgc tcctggatga agcctcgctc aaacgagcca tacctgtcct gatacctgaa
      481 ctggcccatt tttggcgcag ccaatcgatt gttgtcttcg aaagcatgaa agccgttgcg
      541 cccgcaaatc gcctgatcat ccggcttgtt gatttcagca acgctttctc aaattttgca
      601 catctggcag gtgtcccgga aaattccctc gtaaggcaaa atgtacacct caaccgggac
      661 gcgtttccgg agcagatcca gaagttgatc ggcccgtcgt ttctttcgaa catctgttca
      721 aaagagcagc ggactctcga caactacctg aaccgtaatc ccggactctt gaacgtaccg
      781 ctgcccggaa aataatgaac gacctgacac attggcagat tttcagccgg tttgctcgaa
      841 tcggttttac ttcacttggc gcttcaacta cggagattat gcgccagcac cttgtcgatg
      901 agaagcagtg gctaacaacg gatgaattcc gggagaatct ctcactggcg gcaattatcc
      961 cggggccatt tcatgttaat cttgcatgct tctccggcta cagaattctt ggcttcacag
     1021 gatgtattgc ggctgtagtc ggctacgtca ttccaggatt cgtgatcctc gcgctgataa
     1081 tagcatccgt cggtagcgaa cgggtgagcc agctggtgca gaagaatccc gctttcataa
     1141 atggcgtaac cgcaggtatc tgcggacttc tcatgcattc aatcctacgg ctcagcgacc
     1201 gcacgctcga gatgcgaagc gactggctgc tcatactgat cctggcgctt ttgatgcaga
     1261 ccatgcaaat accattcctt ttgctcgcac tcctggccgg actttacttt ctcggccgct
     1321 cgctgtattt tcgctccagg atccgatgac cagttttctg cagtccttac ttgactctct
     1381 cctgatatca gtccagagct tcggaggtat acactcttac tgggccctga tcgaaagaca
     1441 attcgttaca ggctgcaccg aacacatcta tccagaatta ggggaaatat gccggccaat
     1501 gttcaaagct tctctggcgc tggcggaggt aataccggga ccacaagtca acgcagcagc
     1561 atggcttttt tatcatagtt ccggtgtcac tgcagtcttc ggtatattac tcggggcact
     1621 actacccgga attacccttg ctccattact tgtcacattg ctgcgccacg cccggttaca
     1681 aaacctgctc agagaattta tcgctggtgc cagcgtcgct gctatcgcac tactgcttgt
     1741 tttcctgctg cgcctgatcc cgcacaccac agcattctac caacaggtaa cctttggact
     1801 tattggtgca gcctgtttcg tcgtgcggca ctatactaaa gcaaatgtat tggtcataat
     1861 gctcgcggca ggtgttgccg gctggctgtt tctctagggg cttgccccct acggcagcgc
     1921 agccgaatcg ccggtcgtgg cgcccggacc gcccttcttg tccaggatct tccaggggtt
     1981 tgctgccatt tcttttgtga acttgtcgag tgtggttgag gtgctgcgga tgttacgcat
     2041 gatcgcgaca atttcaccct gcgaggcatc aagcaggcca ttcacacggg taattgaacc
     2101 gtttaggttg gtgagcagca tttttacgtc agtcttgttg tcgcccgcca gcgcaccaaa
     2161 agtttccacc atcttgttta ccgtcgacac caggttcgaa atctgcgaaa ccatctggtc
     2221 gagattggtg acgttagtcg caaacagggt atcgtggggt ttgagcgggt acttcttcac
     2281 cagtgacggg gtaagatcga gaatcgcgtc accaatcacg ttctggttgg tgatattgac
     2341 cgttgttccc tggtagatgg tgaattcctt gtctacaagc acgcgcgcct tgaagtgaag
     2401 gtctgtgccg tagatcggga gcacttcctg tacttcgcct atggtaaaat tcttgatctt
     2461 gatctgagtt cctttcttga cgcccgcgag tttttcaaag cgaacccagt attctttacg
     2521 gtcgtcgagc aggttgtagc cggcgataaa gagcagaacc gaaaggccaa aaaccgaacc
     2581 gaccagtaca aaaaggcctg tgaagatatc gttgcggtca aacttcatag cgtcgcgtta
     2641 aagggcggca tcgggccctg tgtccggtag gcacggtatt gctcgaggaa tacattgtct
     2701 gctcttttta tgtcggcaag attaccctgg aacacgacct ctccacggta gaggagcatg
     2761 aatttcaggt cgcgcccgtc aaaaatggtc gggtggtaag aggaaaggat ataggttata
     2821 tttcgcaggc gtatatcttc
//
LOCUS       DAOQJF010000396         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_24274_length_2838_cov_4.335250, whole genome shotgun sequence.
ACCESSION   DAOQJF010000396 DAOQJF010000000
VERSION     DAOQJF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24274_length_2838_cov_4.335250"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLF85_15570"
     CDS             complement(<1..420)
                     /locus_tag="PLF85_15570"
                     /inference="COORDINATES: protein motif:HMM:NF037677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNE21140.1"
                     /translation="MATWERARSAEQKHGRESEILAAARRLLPQKPFERIALSDILQG
                     VSFSRASIYQYFASKEEVYLVLLSEEIRDFGELVEKHCRPAPQGEDRIAEFSTAWAGA
                     LGKEKILLHLLSMAGTVLEKNCSDEILLRSKKTMVLTM"
     gene            781..1683
                     /locus_tag="PLF85_15575"
     CDS             781..1683
                     /locus_tag="PLF85_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21141.1"
                     /translation="MKIEYLNECAVESNNNGQTLLEIALKAKINHTHECGGQARCSTC
                     RVLVLEGAENLGPRTPEETELVQAKGFPDEIRLACQATVHGDVKIRRLVIDQDDIETA
                     YSERGVNRGQERKVAILFSDIRGFTNFSERNLAYDVVHILNRYFKKVGDTVLANNGYI
                     DKYMGDGLMCLFGLEETDPEKICREAVTTAVEMQRQLVKLNEYLKQQFFGEEFRIGIG
                     VHYGEVIVGEIGHPQKHQITAIGDNVNLASRIETATKKVQANILVSDEVRANLGDHFE
                     FRRTFSAQLKGKTGSYRLHEVKCG"
     gene            1852..2655
                     /locus_tag="PLF85_15580"
     CDS             1852..2655
                     /locus_tag="PLF85_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE21142.1"
                     /translation="MADLPPADDTLARKPPPLEVNVEEKLATDLSAFSFFDEAGKPVT
                     LKQLADGKRPIILAPVYYNCPHLCTLTLNGLLQAIETNKRHTLGKDYLVVAYSFNPDE
                     KHQLAELKQKNYLRKLGVVTEQNLPEYQKHWRFLTADVKNIGGISQAMGFKYLKETST
                     NPKKQDPEYVHPAILVVLTPDLRISRYLYGVEYPDTDFRLSLLEAAQGKVSQTIGDKL
                     MLTCYSFDPVKRKYSLVAWRVMRLGAIAVGILLAGFLAVLWYRERRKRA"
BASE COUNT          686 a          771 c          763 g          618 t
ORIGIN      
        1 catcgtcaat accatggttt tcttgcttcg gagcaaaatt tcgtcagagc aattcttctc
       61 gagtaccgtg ccggccatgg acaataaatg cagcagtatt ttctcttttc cgagggcccc
      121 cgcccaggca gtgctgaact ctgctatacg gtcttcgccc tgtggcgccg gtcggcagtg
      181 tttttccaca agttcaccaa aatccctgat ctcttctgaa agaagcacca ggtacacctc
      241 ttctttggaa gcgaagtact ggtagatgct cgcgcgggaa aagctgaccc cttgcaaaat
      301 gtctgaaagg gcaatgcgct caaacggctt ttgcggtaac aggcgccggg ccgcggcaag
      361 aatttcgctt tcgcgcccgt gcttctgctc agctgatctt gctctctccc aggttgccat
      421 gcaaggccgt tgttatgacg ggtacgacat ccggaaccgg caagatcgcc gccgaaacca
      481 aggccgcgct cggagcgaaa cttgtgctgc tcaatcgggc caattctatg gtccgggatt
      541 aggccccatg gccatcaagg gtgaggcgaa accatacggc ctcgaagaat tctataacaa
      601 cccggaaacg cgcgacctgc tctgggaaac aagctgcaag gcaataggcc aggatttcgc
      661 cctatgataa gttgaaattc cccgtatcct gcacgcattg tcacaacccc tgcccgcagg
      721 ctgcaaacga gctaattcag ggccaatggc cctgaatatc gtacattgtc tttacctgcc
      781 atgaaaattg aatacctgaa cgaatgcgct gtagaaagta ataataacgg gcaaaccctg
      841 ctggaaattg ccctcaaggc gaaaattaac cacacacacg aatgcggtgg gcaggcgcgc
      901 tgctcgacct gtcgcgtgct ggtgctcgaa ggcgccgaaa atctcggccc gcgaacgcct
      961 gaagaaaccg agctcgttca ggcgaagggg ttccccgatg aaattcggct cgcctgccag
     1021 gcgactgtcc atggtgacgt gaagatacgc cgcctggtca tcgaccagga tgacatcgaa
     1081 acggcctata gcgaacgcgg ggtcaatcgc gggcaggagc gcaaagtggc gattttattc
     1141 agcgacattc gcggtttcac caatttttcg gagcgcaacc tcgcgtacga cgtggtacat
     1201 attctcaacc gctacttcaa gaaggtcggc gacacggtac ttgccaacaa cggttatata
     1261 gacaagtaca tgggcgacgg cctcatgtgc ctgtttgggc tcgaagaaac cgacccggag
     1321 aaaatctgtc gagaggctgt gacgactgct gtcgaaatgc agcgccagct tgtgaagctg
     1381 aacgaatacc tcaagcagca gttttttggc gaagagttta gaatcggcat cggcgtgcat
     1441 tatggcgagg tgatcgtcgg cgaaatcggt cacccgcaga aacaccagat tacggccatc
     1501 ggcgacaacg tgaacctggc gagccgcatc gagacggcca cgaagaaggt gcaggccaat
     1561 atccttgttt ctgatgaggt tagggcaaac ctgggcgacc atttcgagtt ccgtcgcacc
     1621 ttcagcgcgc agctgaaggg caaaacgggt tcataccggt tgcacgaagt gaagtgcggc
     1681 tgaagtttgc ccgctatgga attccatagc agggtgcaaa aattcaaaaa acacattccg
     1741 ccctgtcgtg cgatcgattt atgcttatat tgcggttgag ctcacatgac aaatataact
     1801 cccacacgcc tgttggcggt ctttggcata attatgtctc atggagtcat catggcagac
     1861 ctaccccctg ccgatgatac tctcgcgcgc aagccgcccc cgctcgaggt gaacgtcgag
     1921 gagaaactgg cgaccgatct ttcggcattc agcttttttg atgaggcggg caagccggta
     1981 acgctgaagc agctcgccga tggtaagcgg ccgatcattt tggcgccggt ctactacaac
     2041 tgcccgcacc tctgcacgct gacactcaac ggtctgctgc aggcgatcga aacgaacaag
     2101 cgccacaccc tgggcaaaga ctacctggtc gtcgcctata gcttcaaccc cgacgaaaag
     2161 caccagctgg cggaacttaa acagaagaac tacctgcgca agctgggggt agtcaccgaa
     2221 cagaacctac ccgagtacca gaagcactgg cgttttctca ccgctgatgt aaaaaacatc
     2281 ggggggattt cacaagcgat ggggtttaag tacctgaagg agacttcgac aaaccccaag
     2341 aagcaggatc ccgaatacgt gcacccggct atccttgtgg tcctgacccc cgacctgcgg
     2401 atttcacgct acctctacgg ggttgagtac ccggataccg atttccggct gtcgctgctc
     2461 gaggccgccc aaggtaaggt gtcccagacg atcggcgata aactcatgct gacgtgttat
     2521 agttttgacc ctgtgaagcg aaaatacagt ttggtggctt ggcgcgtcat gcgtttgggg
     2581 gccattgccg taggtattct attggctggc tttcttgcgg tactgtggta ccgtgaacgg
     2641 cgtaaacgcg catagctgaa cagcgcacga acgagaggga taaatgatca attttcttca
     2701 tgccgccatg gggtttatta cccatgcgac gcctgcgtcg acagtagccc atgaggccaa
     2761 gtttgcagct gactttatca acattataag ccttgtagcg tttatactgg ttgaaggtgc
     2821 gctgatatac tttgtcat
//
LOCUS       DAOQJF010000397         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_24354_length_2833_cov_5.244420, whole genome shotgun sequence.
ACCESSION   DAOQJF010000397 DAOQJF010000000
VERSION     DAOQJF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24354_length_2833_cov_5.244420"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..641
                     /locus_tag="PLF85_15585"
     CDS             153..641
                     /locus_tag="PLF85_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21143.1"
                     /translation="MKLRLLILPALLGAGTLSAAPDAKRLAKVTAELKAIESLGGDRL
                     GYAETILRFLEIENTEYSESRRARPEVSAYDMFSGALAIRESLQTDTIEPGEERGAAI
                     PINTLKGPQTKSHDFKTMLKGRKVQQFPLAGRVPHDWFYAHFSSLTSALNLADYINTR
                     CA"
     gene            680..1288
                     /locus_tag="PLF85_15590"
     CDS             680..1288
                     /locus_tag="PLF85_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21144.1"
                     /translation="MRGLRAVSTKYSRWQTSFAYEYQSAGKSITVFVVNMAGIVTFRF
                     HTWTEGNQIHLATTRDYAERFLSGKRQTTIRRANVLGIYRPSQIIRERSLLVQGHAEA
                     TQRGCFAHLGTVKMASHLFPGEDMTAALEKHYGFRPVCPVGGTYAIKDGKPVHTLLGE
                     PGLARVDFSAIEVLLRDFFATQEFSVDFKFTPHGIMTEIETK"
     gene            complement(1340..2188)
                     /locus_tag="PLF85_15595"
     CDS             complement(1340..2188)
                     /locus_tag="PLF85_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNE21145.1"
                     /translation="MIIRNNLSAVNAHRVLKFNEWETDKTIARLAAGERITKSADDAS
                     GLAVSEKMRTQVQGLRQAERNTEDGISFVQTADGYLNQLGDLLQRVRVLAVQSANGIY
                     TAEDRQLMQVEISQLIDEVDRIASQAEFNRFKVLLGDYARTSKTASMWFHMGANMHQR
                     ERVFVGTMTATAFKLKDNGAAESLSTAAQANDLIGIVDDALGKLLKQRADLGSYSNRL
                     EMSAKGLMNAYENIQASESRIRDADMAEEMVRYTTGQILQQSGTAMLAQANQKAASVM
                     RLLGRE"
     gene            complement(2524..>2833)
                     /locus_tag="PLF85_15600"
     CDS             complement(2524..>2833)
                     /locus_tag="PLF85_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNE21146.1"
                     /translation="RDKRPIWTAAKANSMIGLTDEALYQVNKQRANLGAYYNRLEHAA
                     KGLMNAYENIQASESRIRDTDMAEEMTKFTKNQILIQSGTAMLAQANAKPQSVLQLLR
                     "
BASE COUNT          615 a          822 c          744 g          652 t
ORIGIN      
        1 caccccccac gcagtggggg gtgagggaga aactaccgag cggttgctcc tgccgctggt
       61 aacatactta tcaaacgata aggcgaccac ttgttatgga acccctcagt acaattcggt
      121 tgccctgcaa ggcgtccggt gcaacgtgac agatgaagct ccgcctcctg attttgccgg
      181 cactactcgg agccggcacg ctgtcagcag ccccggacgc gaaacgactg gcaaaagtaa
      241 ccgcagaact taaagccatt gagagcctgg gtggcgacag gctgggttat gcagagacga
      301 tactgcgctt tctcgaaatc gaaaacacgg aatatagcga aagccgtcgc gcacgccctg
      361 aagtgagtgc atacgacatg ttctcgggtg cgctggcgat ccgtgaatcg ctgcagaccg
      421 acaccatcga accgggcgaa gagcgcggcg cagcgatacc cataaacacc ctcaaaggcc
      481 cgcagacaaa atcacacgat ttcaagacga tgcttaaagg ccgtaaggtg cagcagtttc
      541 cgcttgcagg ccgtgtaccg catgactggt tctacgcgca tttctcatcg cttacctctg
      601 cgctcaacct cgccgactac atcaacaccc gctgcgcctg accatagagg cccgcgacag
      661 tgcgagtgcc gaagcactgg tgcgcggatt gcgggcagtc agcacaaaat attcccgctg
      721 gcagacttct ttcgcttacg aataccagtc tgcgggaaaa agcataaccg tcttcgtcgt
      781 caacatggca ggcatcgtaa ccttccgctt ccatacgtgg acagaaggca accagattca
      841 tctggcaacg acacgggatt atgcggagcg ctttctctct ggcaagcggc agacaacgat
      901 ccgccgcgcc aatgtactcg gcatctaccg acccagccag ataatccgcg aaaggtctct
      961 actcgtgcag ggtcatgccg aagccacgca gcgcggctgc tttgcgcatc ttggtacagt
     1021 aaaaatggcc tcgcacctgt ttcccggtga agacatgacg gcagcacttg aaaagcacta
     1081 tggctttcgc ccggtttgcc cggtgggtgg cacttacgca ataaaagacg ggaaaccggt
     1141 gcacacactg ctcggcgaac ccggtttggc gcgggtcgac ttttcagcaa ttgaagttct
     1201 gttgcgcgac ttctttgcga cacaggagtt cagcgtcgat ttcaagttca ccccacatgg
     1261 catcatgact gaaatcgaaa cgaaataatt ttcaacaaaa aagcccgttc cggccaatcc
     1321 ggaacgggct aacatccact tactcgcgac cgagcaggcg catgacgctg gctgcttttt
     1381 ggttcgcctg ggctaacatc gccgtaccac tctgctgcag aatctggccg gtggtgtacc
     1441 ggaccatttc ttcggccatg tcggcatcgc gaatgcggct ctcagacgcc tgtatgtttt
     1501 cataagcgtt catcaagccc ttcgccgaca tctccagccg gttagagtag cttcccaggt
     1561 cggcccgctg tttcagcagc ttgcccagcg cgtcgtcaac gatcccgatg agatcattgg
     1621 cctgcgctgc cgtcgaaagc gattcggccg caccgttgtc cttcagtttg aaggcggtag
     1681 cggtcatcgt tccgacaaac acgcgctcgc gctggtgcat gttggcgccc atgtgaaacc
     1741 acatcgaagc ggtcttcgag gttcttgcat agtcacccaa cagcaccttg aagcggttga
     1801 attcagcctg cgacgcgata cgatctacct cgtcgatgag ctgagaaatc tcaacctgca
     1861 tcagttgcct gtcttcagcc gtgtaaatac cgttggccga ctgtacggcc aaaacgcgta
     1921 cccgctgcag caggtcgccg agctggttaa ggtaaccatc ggctgtctgt acgaagctga
     1981 taccgtcttc ggtatttctt tctgcctggc ggagcccctg cacctgggtt ctcattttct
     2041 ccgaaacggc gagaccagac gcgtcgtctg ctgacttggt gattctttca ccagcagcga
     2101 gacgggcaat cgtcttgtct gtttcccatt cgttgaactt cagaacccgg tgtgcattga
     2161 ctgcgctcag gttgttgcga atgatcatag acactccttt gtctttattt gcggcttcgc
     2221 cgcaaatttt tgtatccgca caggtttggc ttcgccaaac ctgtgcagac gaatctgcag
     2281 cgggacgcat aaagtgtctg tgattgagac atcgacttta gcccatccgt gggctaaagt
     2341 ctctgccact cgcatgacat gtctctccca atcagacacc atgtggaata caccttgatg
     2401 cattccagtg ggcgcccgat ggagagaacc agtacttgaa gtagtgcaaa cacgaaaagg
     2461 cagcctgtgc tgcccctcgc ttaaatcgca ttcagacccg cgctatgcgc gggtctgaat
     2521 gtgttacctc aggagttgca gcactgattg tggcttggcg tttgcctgcg ccagcattgc
     2581 cgtaccgctc tggatgagaa tctggttctt ggtgaatttt gtcatctctt cagccatgtc
     2641 ggtatcgcga atgcggcttt cagacgcctg gatattctcg taagcgttca tcagcccttt
     2701 cgctgcatgc tcaaggcggt tgtagtatgc cccaaggttc gcgcgctgct tgttgacctg
     2761 gtacagggct tcgtctgtca aaccgatcat actgttcgct ttcgctgcag tccagatcgg
     2821 acgcttgtct ctt
//
LOCUS       DAOQJF010000398         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_25096_length_2787_cov_5.454612, whole genome shotgun sequence.
ACCESSION   DAOQJF010000398 DAOQJF010000000
VERSION     DAOQJF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25096_length_2787_cov_5.454612"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLF85_15605"
     CDS             complement(<1..400)
                     /locus_tag="PLF85_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21147.1"
                     /translation="MARAALIFLLTAAGFLAPSLAAHKQLEISAAYQQARYVETFADA
                     LYVGEADYMSSNAAQARAMARFTGFYLPQLNIAYQALAVNSATESRQNTRQNISGYFS
                     SYASAMASTDTGILRWHYGFAALVTFGYRNY"
     gene            complement(425..1450)
                     /locus_tag="PLF85_15610"
     CDS             complement(425..1450)
                     /locus_tag="PLF85_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21148.1"
                     /translation="MLKKLLIIVLALPLTVFAQNKGKIPLKNTAGPAKVNPPVTAEQP
                     VTPTLPDNSAVTPVAPPAYTERFEIFASFSQGTRLTGYTDPRYVANDKTDYLESPAMT
                     ANIEFSGKIPYRIKGSPTDSALQDIFAGTEYLRIGGQLTGWSILNSSSVSIPKTGAPI
                     SADAVNANLNATTRPGLGFFFGFGKRDFEIDFGITTALAFEHEGSRTRRVVDANGNVT
                     GATEEVSGRGLFIANAFVLPTFRLAWGGRDDLQFFVAAGRETFEYQRDYVQTYFRIPV
                     AAFLKVDFGVGLFPNATLFLQPNLVFGNIMVGVRGGITLNYYESELRRVSINDALYFA
                     LSASGRF"
     gene            complement(1503..1970)
                     /locus_tag="PLF85_15615"
     CDS             complement(1503..1970)
                     /locus_tag="PLF85_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE21149.1"
                     /translation="MAKTAAVSRPEAYLKGLPAEKREALSGLRAVIRKNLPKGFEETI
                     SYGMIGYVVPHKLYPGGYHCDPKLPLPFMSIAAQKNFFAFYHMGLYADKELMQWFTNE
                     FRKHSEGKLDMGKSCVRFKKGEDIPLKLIGQLVKKMSVKKWVKTYEKALRPRK"
     gene            complement(1972..>2787)
                     /locus_tag="PLF85_15620"
     CDS             complement(1972..>2787)
                     /locus_tag="PLF85_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNE21150.1"
                     /translation="LLLSLKAKGSLPKISDRLGYGIRTNSESLLGVTTERRDLNFAKG
                     IAIGSILHVDDHTHLEVVRYPEGSGFFRMSMVPAVSGSNFFVRIGRIVWQYLRHPWKY
                     LKAIAVPDWAKYTQIMLFMQSLDSTMRLTRGWLRMKTALDKGEAPTSDIPMAHELAHK
                     FAQKTKGSVHALATEALTGLASTAHILGGSCMGDSAATGVIDSSNRVFGYDNMYICDG
                     SMISANIGVNPSLTIMALTERAMSLIPKKTAGKVISASAKKVTKGRATAKTKR"
BASE COUNT          632 a          721 c          791 g          643 t
ORIGIN      
        1 cgtaattacg gtagccgaat gtgacgaggg cagcaaagcc atagtgccag cggagtatgc
       61 cggtgtcagt tgaggccatg gcgctggcat aggatgaaaa atagccgctg atattctggc
      121 gggtattctg ccgggactcg gtggcagaat tgacggcaag cgcctggtac gcaatattca
      181 gttgcggaag ataaaaaccg gtgaagcgcg ccatggcgcg cgcctgcgct gcattgctgc
      241 tcatgtagtc ggcttcaccg acataaagag cgtcggcaaa agtctcgacg taacgcgcct
      301 gctggtaggc tgcagaaatt tccagctgct tgtgcgcagc cagggaaggc gccaggaagc
      361 ccgctgcggt cagcaggaat ataagggctg ctcgcgccac gatcaggcta ttggcaacac
      421 caggttaaaa acgccccgag gcagaaaggg caaagtacag cgcatcgtta atggaaacac
      481 gcctcagctc gctctcgtag tagttaagag tgatgccgcc gcgtacgccc accataatat
      541 tcccaaaaac aaggttgggc tgcagaaaca gggtcgcatt gggaaacaaa ccgactccga
      601 aatccacttt caaaaatgcc gccaccggaa tgcggaaata agtctgcacg taatcgcgct
      661 ggtattcaaa tgtttcacgt cctgccgcca caaaaaactg caggtcgtcg cgcccacccc
      721 atgccaggcg aaaagtcggt agcacaaacg cattcgcgat gaaaagaccg cgccctgaaa
      781 cttcttcggt cgcgccggtg acattgccgt tggcgtcgac aacgcggcgt gtcctggagc
      841 cttcgtgttc aaaggcaagt gcggtggtaa taccgaaatc aatttcgaag tcgcgcttac
      901 caaaaccaaa gaagaagcct aaaccggggc gggtcgttgc attcaggttc gcgttgaccg
      961 cgtctgcgct gataggcgca cccgttttcg gaatagagac actgctgctg ttcagtatac
     1021 tccagccagt gagctgaccc ccgatacgca ggtattcagt gcccgcaaaa atatcctgca
     1081 aggcagaatc tgtggggcta cccttgatgc gatagggtat cttgccggaa aattcgatgt
     1141 ttgcagtcat tgccggcgat tcgagataat cggttttatc attcgcgacg tagcgcgggt
     1201 cagtgtaacc tgtaaggcgt gtgccctgcg agaaagaagc gaaaatttca aaacgttcgg
     1261 tataggctgg cggcgcaacg ggcgtaaccg cgctattatc ggggagtgtt ggcgttacgg
     1321 gttgttctgc agtgacaggt ggatttactt ttgcaggacc ggcagtgttc ttgaggggga
     1381 ttttaccctt gttttgggcg aagacagtca gcggcagcgc aagtacaatg atcagtagtt
     1441 tcttcagcat acaaccctgt aaccggtagt atgccttgcg gcaagagatt ttttacaggc
     1501 tgctatttcc gtggacgtaa ggctttttcg taagtcttga cccacttctt tacagacatt
     1561 ttttttacca gctggccaat gagcttcagg ggtatatcct cccctttctt gaaacgaaca
     1621 cagctcttgc ccatgtccag cttgccctct gagtgctttc ggaattcatt cgtaaaccat
     1681 tgcatgagct ctttgtcggc gtagaggccc atgtggtaga acgcgaaaaa attcttctga
     1741 gcggcaatgc tcatgaaagg cagcggcagc ttcgggtcac agtggtagcc gccggggtag
     1801 agcttgtgcg gcaccacgta accgatcatg ccgtagctga tggtctcttc aaaacctttt
     1861 ggcaggtttt tgcgtataac cgcgcgcaag cctgagaggg cctcgcgttt ttcggcaggc
     1921 aggcccttca ggtaggcttc gggccggctg accgctgccg ttttcgccat ctcagcgttt
     1981 cgtttttgcg gttgcgcggc ccttggtgac tttttttgcg ctggcggaaa ttaccttgcc
     2041 cgcagtcttc tttggaatca gtgacatcgc acgttcggtc agcgccatga tcgtcagcga
     2101 cgggttcaca ccgatattcg ctgaaatcat cgagccatcg cagatataca tgttatcgta
     2161 gccgaacacc cggttgctgc tgtcgatcac accggttgct gccgagtcac ccatgcaaga
     2221 gccaccaaga atgtgcgcgg tgctagcgag gcctgtgagc gcctcggttg cgagagcatg
     2281 caccgaaccc tttgtttttt gtgcaaactt gtgcgcgagc tcgtgcgcca tcggtatgtc
     2341 agatgtcgga gcttcccctt tgtcgagagc cgttttcatg cgcagccagc cgcgcgtcag
     2401 ccgcatggtc gaatcgaggc tctgcatgaa cagcatgatc tgcgtgtatt tcgcccagtc
     2461 gggtactgct attgccttca ggtacttcca cgggtggcgc aggtattgcc agacaatgcg
     2521 accgatgcgg acaaaaaagt tggaacctga aaccgcggga accatagaca tcctgaaaaa
     2581 gccagaacct tcaggatagc gtacgacttc caggtgagtg tggtcgtcga cgtgcagaat
     2641 tgagccgata gcgataccct ttgcgaaatt gagatcgcgg cgctcggttg tgacgccaag
     2701 caggctttct gagttggtgc gtatgccgta accgagccgg tcagaaattt ttggcagcga
     2761 accttttgcc ttcagagaga gcagcag
//
LOCUS       DAOQJF010000399         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_25136_length_2784_cov_5.306339, whole genome shotgun sequence.
ACCESSION   DAOQJF010000399 DAOQJF010000000
VERSION     DAOQJF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25136_length_2784_cov_5.306339"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLF85_15625"
     CDS             complement(<1..574)
                     /locus_tag="PLF85_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21151.1"
                     /translation="MAERIDIDGVRFRVGADLKDLEAGFKKGEQLGAEAGKKTGSKFS
                     QAFGAVVSAVAGAQIVGAMKTAFDAANKLEKTMLGLQATAKLTGQSFAVLSKSVNDLA
                     KDGVMNIDQAAQSMKVLAAQGVNAQKSFEFLEAAKKVSAFNNIVGDAGQGVQDFVKFL
                     QTGSAELAENMDPSIVAVVKRLGGYAAVTAD"
     gene            complement(559..777)
                     /locus_tag="PLF85_15630"
     CDS             complement(559..777)
                     /locus_tag="PLF85_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21152.1"
                     /translation="MLKKILTVFFAVVVGPIWLTVMVLAWLRYLLSVRKYSGQFAVLY
                     GRDAGQIYRREAGMSFLQFAGGSKWPKE"
     gene            complement(771..1337)
                     /locus_tag="PLF85_15635"
     CDS             complement(771..1337)
                     /locus_tag="PLF85_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21153.1"
                     /translation="MKYIGAGVFLRDKVHVSDVYTCIEASRHASTVKGWLKLHYVKRI
                     VSALTGRRAWFWQVGLVNRLMQAAGFNSEHSAGDADDAKAKLDKVCAWIGARLNMTME
                     RVASELSVDEIQPTVYAIVKRDLERNLDMIKAQHLPEKFSKEILDEMRKLSQEVTATN
                     KEVKQIKQDLDRYGRSKRTFTAGMLQPC"
     gene            complement(1337..2449)
                     /locus_tag="PLF85_15640"
     CDS             complement(1337..2449)
                     /locus_tag="PLF85_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21154.1"
                     /translation="MAITNAQIAAIDKQRMEIIFDDVNLGAVKPDTLRVQVSGNYQPV
                     TIDQLQGVVKEWQEGFTINVQVTLMDVSPSFIYGTLMSGQVFAKTAASGDLFVGFGNR
                     KIDMLNIAAELRLHEYGVDDTTRTLDWLFWKAKAKFDGTEFAYGATTIKELVVKFTIY
                     PDLDKAVDYQYGGYGDARGYDAEAVPKGVWIATGKTAQVPGKHLTALTLNPSDLQDLQ
                     AFAAYSSNGTITAAIDDATDINSTDTAIVYDTLAGGNIVAGDYILINSEYMRVTANDT
                     GTNTLTVQRAIWGSTAAAHLDNAVITVQTAVSIVRVTNWATWASSVTADVTVGTTFGG
                     SGTAAIGRIAHVSSGSSNVTATVNTKASPNLVVTTN"
     gene            complement(2458..>2784)
                     /locus_tag="PLF85_15645"
     CDS             complement(2458..>2784)
                     /locus_tag="PLF85_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21155.1"
                     /translation="VRVLVDVNPRPDMDLAQATRSNIMQGFLMMYCFAKSDSLAVSGL
                     KDWWTTIAGDGGRILHNIEGKVTLGTVEFLHWRAVGGFQGFATKHGGSVWMCDQMVEY
                     AARVLP"
BASE COUNT          633 a          815 c          655 g          681 t
ORIGIN      
        1 cgtctgcggt gactgctgca tatccgccaa ggcgcttgac aacagccacg atactcgggt
       61 ccatgttctc agccaattca gccgacccag tctgaagaaa ctttacaaag tcctgaacgc
      121 cctgcccggc gtcgccgaca atgttattaa acgcagagac ctttttcgct gcttcgagaa
      181 actcgaaaga cttttgcgcg ttgacaccct gcgccgctag taccttcatc gactgcgccg
      241 cttggtcgat gttcatcaca ccgtcttttg ccaagtcatt tacagatttt gagaggacgg
      301 caaaagattg cccggtgagc ttggcggtag cctgaaggcc aagcatggtt ttttcaagtt
      361 tgttggcagc gtcgaaagcc gttttcatgg ccccgacaat ctgagcacca gcaacggcag
      421 aaacgaccgc gccgaacgct tgcgagaatt tagagcctgt tttcttgccg gcttctgcgc
      481 ctaactgctc gccctttttg aagcccgctt cgaggtcttt aagatcggca ccaacgcgga
      541 accggacgcc atctatatct attctttcgg ccatttgcta cccccggcga attgtagaaa
      601 cgacattccc gcttctctgc ggtagatttg cccggcatca cggccatata aaacagcgaa
      661 ctgaccgcta tacttgcgca cgctcaaaag atagcgcagc cacgccaata ccatgacggt
      721 caaccaaata ggaccaacca ccacggcaaa gaaaactgtc aaaatcttct tcagcatggc
      781 tgcaacatcc ccgctgtgaa cgtgcgttta cttctgccgt acctgtccaa gtcctgtttt
      841 atctgcttta cctctttgtt cgttgccgtg acttcctgcg aaagtttccg catctcgtcg
      901 agaatctctt tagaaaactt ctctggtaaa tgctgcgcct tgatcatgtc aaggtttcgc
      961 tccaagtcac gcttcacaat tgcgtagact gttggctgta tttcgtcaac cgataactca
     1021 cttgccacgc gctccatagt catatttaac cgcgcgccga tccacgcgca aaccttgtca
     1081 agtttggcct tggcgtcatc ggcatcgcct gcgctgtgct cgctgttgaa ccctgccgcc
     1141 tgcatgagac gatttacaag gcccacctgc cagaaccacg cccgccggcc agtcaaagct
     1201 gacactatgc gtttgacata atgcagcttc aaccagccct taacggtgga agcatggcgc
     1261 gaagcttcga tacacgtgta cacgtcggag acgtggacct tatcgcgtag aaaaacccct
     1321 gcgccaatgt acttcattag ttagttgtta ccacaaggtt cgggcttgcc ttagtgttca
     1381 ctgttgcagt gacgttagac gaacccgaag acacatgagc gatacgaccg atagccgcag
     1441 tgccagaacc cccgaaagtg gtgcccacag taacgtcagc agtaacagag cttgcccatg
     1501 ttgcccagtt tgtaacccgg acgattgaca cagctgtctg aacggtgatt acagcattgt
     1561 caaggtgcgc cgctgctgta cttccccaga ttgcacgctg caccgtcagc gtatttgtcc
     1621 cggtgtcgtt cgcagttacc cgcatatatt ccgaattgat cagaatatag tcacccgcca
     1681 caatgttgcc accggccagc gtatcataca cgattgctgt atcggtagag ttgatatctg
     1741 tcgcgtcgtc gatagcggca gtgatggttc cgtttgacga gtacgccgca aacgcctgca
     1801 ggtcctgaag gtctgacggg ttcagggtaa gggctgtcag atgcttgccg gggacctgtg
     1861 cagtcttacc ggtagcaatc cagacgcctt ttggcacggc ttccgcatcg tacccgcgcg
     1921 cgtcgccgta tccgccatat tggtagtcaa cggccttgtc gagatccggg taaattgtga
     1981 attttaccac aagctcttta atggtcgtcg cgccataggc aaactcggtt ccgtcgaact
     2041 tggccttagc tttccagaac agccagtcga gggtacgagt tgtatcgtct acgccgtatt
     2101 catgcagcct cagttcagcc gcaatgttga gcatatcaat cttacgattg ccgaaaccca
     2161 caaacaggtc accagacgcg gcagtctttg caaagacctg accgctcatc aaggtcccgt
     2221 aaatgaacga cggcgaaacg tccatcagtg tcacctgtac attgatggtg aatccctctt
     2281 gccattcctt cacgacgcct tgaagctggt cgattgttac cggctgatag ttaccagaca
     2341 cctgcacgcg cagtgtatct ggtttgacag cgccgagatt aacgtcgtca aagatgatct
     2401 ccatcctttg tttgtcaatc gcggcgattt gagcatttgt tattgccatt tattctccta
     2461 tggcagcacg cgtgcggcgt attcgaccat ttggtcacac atccacacac taccgccgtg
     2521 ttttgtcgca aacccctgaa agccccctac tgcgcgccaa tgaagaaact ccacagtgcc
     2581 cagcgttact ttcccctcga tgttgtgcaa aatccgaccg ccatccccgg cgatcgtagt
     2641 ccaccagtct tttaaccccg agaccgctag actatccgat ttcgcaaaac agtacatcat
     2701 cagaaatcct tgcatgatat tcgaccgggt ggcctgcgca aggtccatgt cagggcgggg
     2761 gttgacatcc accaaaaccc taac
//
LOCUS       DAOQJF010000400         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_25292_length_2774_cov_4.785583, whole genome shotgun sequence.
ACCESSION   DAOQJF010000400 DAOQJF010000000
VERSION     DAOQJF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25292_length_2774_cov_4.785583"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLF85_15650"
     CDS             complement(<1..723)
                     /locus_tag="PLF85_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE21156.1"
                     /translation="MKYRSMTEIIGNTPHLKLNRLYNEDFEVWVKLERQNPGASIKDR
                     IALAMIEDAERRGILKPGSVIIEPTSGNTGIGLALVAAAKGYKLTLVMPESMSVERRA
                     IMAAYGAQFELTPREKGMKGAIEKATELVQSTPNAWMPQQFENEANIEVHRKTTAQEI
                     LRDFPEGLDVMITGVGTGGHLTGVTEVLRQKWPNLQVYAVEPAASPVISGGQPGPHPI
                     QGIGAGFIPKNLRKDLLTGVIQV"
     gene            837..1364
                     /locus_tag="PLF85_15655"
     CDS             837..1364
                     /locus_tag="PLF85_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21157.1"
                     /translation="MSRRRCGVSLLLLLVACVQPSCRLERREDFDFSVDTPLRCGIFR
                     SYYFVRDIREEVYPDLGLVLANSARLGLSSTQQSRLTTLARECTELCEVRKDHLRLMQ
                     EDIKAKLALNEIKGNLALLAREVQAFERAKAEWLRGHEARYHAGTSILTEAQHARWKQ
                     AESYLKPFPLQKEER"
     gene            1366..2619
                     /locus_tag="PLF85_15660"
     CDS             1366..2619
                     /locus_tag="PLF85_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21158.1"
                     /translation="MLLWQYIRSWPWWAISLSLGINILLLFAAVMAITIFRDRLPIVV
                     VSFLFFYIFMAQPLKDWLISRMGMDSKYSVQSGFITFDNRVSRIMRLKDVLEFLGWLI
                     RAWKLSRLRLLVFDEEDLVFFVNRQKRVRRMRLKDDLTEQFRQELSRLNGAKPVTALS
                     PALKAYMQSRKVRFVAPVLFRERLIGLVGFSEVVDRARLPLVDHAAHRIGLALENERL
                     ERTVPRSEFLSKEFRLAEKIERHLSGASRYSAGRFTVQKLDTAWEKKHFSAIFGCARS
                     DDPAEETHFVMLLRLSVASTRSNALQLFSTQGYFYSLIRGQRDVAGLSAELHRNLRVN
                     ENRSIVLEGFLISLHPAQNLMRIQAFGRHLAYRDESGWSWIRETPPLGAEDFEQGELI
                     ELRPRKDVIVSMREYPLLLISGGMG"
BASE COUNT          546 a          754 c          794 g          680 t
ORIGIN      
        1 tacctgaata actcctgtga gcaggtcttt cctgagattc tttgggataa aacctgcgcc
       61 gataccctga atcgggtgtg gccctggttg gccacccgaa atgacgggtg aggccgcggg
      121 ttcgaccgcg taaacctgca ggttaggcca tttctggcgc aggacctcgg tgacaccggt
      181 caggtgaccg ccagtgccga cgccggtgat catcacatcc agcccttcgg gaaaatcccg
      241 caggatttcc tgcgcagtgg tcttgcggtg tacttcgatg ttggcttcgt tctcaaactg
      301 ctgcggcatc caggcattgg gggtagactg cacgagttcg gtggcttttt caatagcacc
      361 tttcatgcct ttttcgcgcg gtgtgagctc gaactgcgcg ccataagctg ccataatagc
      421 ccggcgctcg acagacatgc tctcaggcat gacgagggtc agcttataac ccttggctgc
      481 agccaccagc gccaggccga tgcctgtatt acccgaggtt ggttcgataa taacgctgcc
      541 cggtttcagt atgccgcggc gttctgcgtc ttcaatcatg gcaagcgcga tacgatcttt
      601 aatgctcgca ccgggattct ggcgctcgag cttcacccag acctcgaaat cttcgttgta
      661 gaggcggttg agcttcaaat ggggggtatt gccgattatt tcggtcatgc tacggtattt
      721 catgcgcccc gatgcgtcag gcagtcggct cggcaagaaa ttatgcaggt aaaaaatccg
      781 gcgcgttatt gcagctttgt tatttacgcg tgtgagccgc gggtttttag cgtaatatgt
      841 cccgcaggcg atgtggagtt tctcttctgc ttttgctcgt ggcgtgtgtc cagccttcct
      901 gccgccttga gcggcgcgag gattttgatt tttctgtcga tacgccgctg cgctgcggaa
      961 tttttcgctc gtactacttc gtccgtgaca tacgcgagga ggtatatcct gaccttggcc
     1021 tcgtgttggc taactcggcg cgcctgggat tgagcagtac gcagcagtct cgactgacga
     1081 cgctggcgcg tgaatgcaca gagctctgcg aagtcaggaa agaccacctg cgccttatgc
     1141 aggaagacat caaggctaag cttgcgctca acgaaattaa gggaaatctc gcattgctcg
     1201 cgcgtgaagt acaggcgttt gagcgggcca aggccgagtg gctgcgcggg cacgaagcgc
     1261 ggtaccatgc cggaaccagc atcctgaccg aagcgcaaca tgcgagatgg aaacaggccg
     1321 agtcatacct gaaacctttt ccactgcaaa aagaggagcg ctgatttgct cctgtggcaa
     1381 tacatacgtt cctggccctg gtgggcaatc agtctctctc tcggcatcaa tatccttctg
     1441 ctttttgcag cggtaatggc tatcaccatc tttcgcgatc gcttgcccat cgttgttgta
     1501 tctttcctgt tcttttacat attcatggcg cagccgctga aggactggct gatttcgcgc
     1561 atgggtatgg attcaaagta ctcggtgcag agcggtttta tcacgtttga taaccgtgtt
     1621 tcacgcatca tgcgcctgaa agatgtgctc gagttcctgg gctggctgat ccgggcgtgg
     1681 aaattgtccc ggctgcgttt gctggttttc gacgaagaag acctggtttt ttttgtaaac
     1741 cggcagaaac gcgtcaggcg catgcgcctc aaggacgacc tgacagaaca attcaggcag
     1801 gagttgtccc gcctgaatgg tgcaaaaccc gtaacggcgc tttctcccgc actaaaggca
     1861 tatatgcagt cgcgcaaggt gcggttcgta gccccggtgc tttttcgcga gcgactcatt
     1921 ggcctggtag gtttcagcga ggtcgtcgac cgggcgcgcc tgcctttggt cgatcatgct
     1981 gcacaccgga tcggccttgc gctcgagaat gaacggctgg aacgcacggt cccccgcagt
     2041 gaatttctca gcaaggaatt tcgccttgca gaaaagatcg agcgccacct gagcggggct
     2101 tcacgctatt ctgccggccg ctttaccgta cagaagctgg acacagcctg ggagaaaaaa
     2161 catttctcgg ctatcttcgg ctgcgccagg tctgacgatc cagccgaaga aacccatttt
     2221 gtcatgctgc ttcgcctcag cgtcgcttcg acccgcagca atgccctgca gctcttttcg
     2281 acccagggct atttttattc gttgattcgg ggacagaggg atgtagcggg gctttcggct
     2341 gaattgcacc gcaaccttcg ggtaaacgaa aaccgctcca tcgtgctcga agggttcctg
     2401 atttccttgc acccggcgca aaacctgatg cggatacagg catttggccg tcacctggca
     2461 tatcgcgacg agagtgggtg gagctggatc cgtgagacac cgccactggg tgcagaagac
     2521 ttcgagcagg gcgaactgat cgaactgcgt ccgcgcaaag atgttatcgt gagtatgcgc
     2581 gaatatcctc tgctcctcat cagcggaggt atggggtgag tcgtgtcctg tggtttggtt
     2641 cgatggtgct gctcctgctg ggcgtggtac tgcagattgc tgcgacgcag cgctatattg
     2701 cgcaggccca cccgcaggta ctcaccacac ccaacgggct ggcattgatt ccgctcacgt
     2761 ttagtattcc tgat
//
LOCUS       DAOQJF010000401         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_25391_length_2768_cov_6.078511, whole genome shotgun sequence.
ACCESSION   DAOQJF010000401 DAOQJF010000000
VERSION     DAOQJF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25391_length_2768_cov_6.078511"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..620
                     /gene="rpsD"
                     /locus_tag="PLF85_15665"
     CDS             3..620
                     /gene="rpsD"
                     /locus_tag="PLF85_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNE21159.1"
                     /translation="MGPACKLCRREGLKLFLKGQKCMTDKCIFNKRKNPPGPPPKRKP
                     KASGYATQLREKQKVKRVYGVLERPFRNYFERATRMKGITGENLLILLERRLDNALYR
                     LGLASSRSQARNFITHGHIRVNGRRVDIPSYKVAIGDSISLGDKLKANKVVGENVEMA
                     KNAGLIADWMELGEDGKTAKVVKLPSRENVDIPIKENVIVELYSK"
     gene            638..1618
                     /locus_tag="PLF85_15670"
     CDS             638..1618
                     /locus_tag="PLF85_15670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNE21160.1"
                     /translation="MAKTAPKNLLKGLKRPKSAEFQHVETRPDYGKFVAEPFEKGFGV
                     TIANSLRRVLMSYIEGAAIVAVKIEGVSHEFSTMPGIKEDVTRLILNLKRVRLKMEGQ
                     GPLTLEIEKKGAGVLMAGDLAVSNSVEIMNPDLVLAHMNEDANLRMTVQIDKGRGYLP
                     AEITKKMIDEVGVIPIDALFSPITKVNFDISETRVDQRTDYDKVTFEIWTDMTISPED
                     AMAYAAKILKEHLTVFINFAEEMYEEEEEFDETDERLKKAMQVALEDMELSVRSIAVL
                     RSLDMRNVKDIVTRYEDDLRKSKHYSDKVLLQLKSKLANMNLSFGMRDGR"
     gene            1642..2145
                     /gene="rplQ"
                     /locus_tag="PLF85_15675"
     CDS             1642..2145
                     /gene="rplQ"
                     /locus_tag="PLF85_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNE21161.1"
                     /translation="MIKGNKVKHLNKKVGHRQAMLGNLVQSLFYHENITTTVAKAKVA
                     RQIAEKLITRARANQNASLSPAEKVHNVREAGKIITDKEILHKLFNDIAPRFATRPGG
                     YTRILRVGQRISDASEMAVLELVERKALAQLKDERKVIRTARKKKSGKTAEKKPAEAK
                     EKKTKKK"
     gene            2281..>2768
                     /locus_tag="PLF85_15680"
     CDS             2281..>2768
                     /locus_tag="PLF85_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNE21162.1"
                     /translation="MSVKTINDIRKAYLGYFESKKHMRLPSASLLPAQDPTLLFTTAG
                     MVPFKEYFSGARVPPSKRVATVQKCLRTTDLESVGRTKRHLSFFEMLGNFSFGDYFKK
                     EAIEFAWEFSTQFLPFPKERIWISIFENDDEAHEIWNKHIGVPAERIVRLGRADNFWG
                     PAG"
BASE COUNT          781 a          644 c          740 g          603 t
ORIGIN      
        1 caatcggacc agcgtgcaaa ctgtgcagaa gggagggatt aaaactcttc ctgaaaggcc
       61 agaagtgcat gacagacaaa tgtatcttta acaagcgtaa gaacccaccg ggtccaccgc
      121 caaagcgtaa accgaaggct tcaggatacg caacacagct gcgcgaaaag cagaaagtga
      181 agcgcgtgta tggtgttctt gagcgtccat tccgcaacta ttttgaacgt gcaacacgca
      241 tgaagggcat caccggtgaa aacctgctga tcctccttga gcgtcgtctc gataacgcac
      301 tgtaccgcct cgggcttgct tcttcgcgct cgcaggcgag aaactttatt acccacggcc
      361 atatccgggt aaacggcaga cgagtggata ttccatcgta caaagttgct atcggtgaca
      421 gtatttcgct gggcgataag ctcaaggcaa acaaggttgt aggcgaaaac gtcgagatgg
      481 cgaaaaatgc cggtctgatt gccgactgga tggagctcgg tgaagacggg aaaactgcga
      541 aagttgtgaa actgccttcg cgtgagaatg tggatattcc gatcaaagaa aacgtgatcg
      601 tggaattgta ctcgaaataa ggaccggagg gcgatagatg gcgaaaacag caccaaaaaa
      661 cctgctcaaa ggtctcaagc ggcctaaatc ggctgaattc cagcacgtag aaaccaggcc
      721 tgattacggt aagttcgtag cagaaccttt tgaaaaaggc tttggcgtaa ccattgccaa
      781 cagtctgcgc agggtgctca tgtcttacat cgagggcgct gcgattgtag cggtcaagat
      841 cgaaggcgtc agccacgaat tctctacgat gcctggcatc aaagaagacg ttacgcggtt
      901 aattcttaac ctgaagcgtg ttcgcctcaa gatggaaggc cagggaccac tgacccttga
      961 gatcgagaaa aaaggtgcgg gtgtactcat ggctggtgat cttgctgtat cgaacagcgt
     1021 tgagatcatg aatcctgatc ttgtgctcgc gcacatgaac gaagatgcca acctgagaat
     1081 gacggtgcag atcgacaagg gtcgtggtta tctgcctgct gaaatcacca agaagatgat
     1141 cgatgaagtg ggtgtaatcc ccatcgatgc gctcttctct ccgattacca aggttaactt
     1201 tgatatttct gagacccgcg ttgaccagcg tactgactac gataaagtaa catttgagat
     1261 ctggacagac atgaccatct ctcccgaaga tgcgatggca tatgctgcta agatcctcaa
     1321 agagcacctg acagtcttca tcaattttgc agaagaaatg tatgaagagg aagaggaatt
     1381 cgacgaaaca gacgagcgcc tcaagaaggc aatgcaggtt gcactcgaag acatggaact
     1441 ttctgtgcgg agtattgcgg tactgcgtag cctcgatatg cggaacgtca aagatatcgt
     1501 tactcgctat gaggacgatc tgcgcaagag caagcattac tcagacaagg ttctgctgca
     1561 gctgaaaagc aagctggcga atatgaacct gtcttttggc atgcgcgacg gccgctaagc
     1621 ggccattgaa ccggagatac aatgattaag ggaaacaaag ttaaacacct gaataagaaa
     1681 gtagggcatc gccaggcgat gcttggcaac cttgtgcagt cgctgttcta ccacgagaac
     1741 attacaacaa cggtagctaa ggcaaaagta gcgcgccaga tcgctgagaa gctgattacc
     1801 cgtgcgcggg caaaccagaa tgcatcgctc tctcctgcag aaaaagtgca caacgtgcgt
     1861 gaagctggaa agatcattac agataaggaa attctccaca aactgttcaa tgatattgcg
     1921 ccacgctttg caacgcggcc cggtggctat acacgtatcc tgcgcgttgg ccagcgtatt
     1981 tctgatgcat cagaaatggc tgttcttgag ctggtagagc gtaaggctct ggcgcagctg
     2041 aaggacgaaa gaaaggtcat tcgcactgcc cgtaagaaga agtctggaaa gacggcagaa
     2101 aagaagccgg ccgaagcgaa agagaagaag accaagaaaa aatagaactg ccagttagcg
     2161 cagttcagaa agcgcgcctg tggcgcgctt tttttttatc aatgcagacc gtacaaaggc
     2221 gcattgttct tgcggcccca tttcatatac agggatgcac catcaggcac cggagggaaa
     2281 atgtcggtca agactatcaa tgatataaga aaagcgtatc tcggttactt tgaatccaag
     2341 aagcacatgc gtctgcccag tgcatccctg ttgcccgccc aggatccgac gctgctgttc
     2401 acaaccgcgg gtatggttcc attcaaggaa tatttttccg gggcacgtgt cccgccatcg
     2461 aagcgtgttg cgactgttca gaagtgcctg cggactactg accttgaatc cgtcgggcgc
     2521 acgaagcgac acctttcgtt ttttgaaatg cttggcaatt ttagttttgg tgattacttt
     2581 aaaaaagaag cgattgaatt cgcctgggag ttctcgaccc aattcctacc gttccccaaa
     2641 gagcgcatct ggatcagtat atttgagaat gatgatgaag cgcacgaaat ctggaacaag
     2701 catatcggag ttccggccga gcgtattgtg aggctcgggc gcgcagataa tttctgggga
     2761 ccggcggg
//
LOCUS       DAOQJF010000402         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_25747_length_2747_cov_3.231798, whole genome shotgun sequence.
ACCESSION   DAOQJF010000402 DAOQJF010000000
VERSION     DAOQJF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25747_length_2747_cov_3.231798"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..553)
                     /locus_tag="PLF85_15685"
     CDS             complement(8..553)
                     /locus_tag="PLF85_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21163.1"
                     /translation="MLRNIKEGIYRGIGLLVVLGTAVFAVSVTGTIKTWSSGETLTAT
                     DLNTTVQSLKTAVESATQLVEFGRIRNTETYETYYGTLTSGTTASAAVASTMTRSGTI
                     KNAKLMIYGTMPFGCVMTLTKNGSDTAISMSAANGAGASTITDADTVTFIAGDTLGWK
                     NYCAGSNGNTAIVPMIVSFEF"
     gene            869..1132
                     /locus_tag="PLF85_15690"
     CDS             869..1132
                     /locus_tag="PLF85_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE21164.1"
                     /translation="MKARLSVADQKEFDRFLAQIGERLKARRAELGLRQEDLDLAPCP
                     MDVRKYQRIEYGQMNFTMQSLYVLSKNLKLAPEKLLTGLLEPS"
     gene            complement(1198..1500)
                     /locus_tag="PLF85_15695"
     CDS             complement(1198..1500)
                     /locus_tag="PLF85_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE21165.1"
                     /translation="MDSLVDEIGEDEKHPLAPLLETVGSLLEIYEAEQVKVPDVSAVD
                     MLKILMKEHDLTQADLKEIASQGVLSEILHKKRALNLRQIKLLAKRFNVSPAAFID"
     gene            complement(1553..2638)
                     /locus_tag="PLF85_15700"
     CDS             complement(1553..2638)
                     /locus_tag="PLF85_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21166.1"
                     /translation="MSHQMAYAFERIVFFQASAADRERIHALQLLHPALTSTRTTFIH
                     HAAELDSLEPTALLVCCGNSAAHAYRTALDHGLTCLISFEDFLTTAAQLIQLTASAAF
                     GEVVLAFLPEALRRQASGIFSLYGFNPVAVDSSQALLRELKHGAAIVVFDQDMPSLRG
                     RVNESREKIFSLLRAERRAHRQLAVVIIKDFDQGSLFGDMTTMAKDVSNAMLSPAEFL
                     EYIRNYLSDFHAAHATWKLRIGHTTGLAHSTYTGRPSPCLGLADVKCGFQLMHDKAYH
                     EYTHAREQMLIETRDLAARMAVTEWLFDKALAQEAESVGKSLAVKTEMPRLFDIQRAH
                     AQPAQQGPQPPLADGAKAGESENPQKN"
BASE COUNT          639 a          739 c          701 g          668 t
ORIGIN      
        1 gtgtctctta gaactcgaag cttacgatca tcgggactat agcggtatta ccatttgaac
       61 cggcgcagta atttttccag ccgagagtat cacctgcaat gaatgtcaca gtatccgcat
      121 ctgtgatcgt tgatgcccca gcaccattag cagccgacat actaatggca gtatctgaac
      181 cattttttgt aagcgtcatg acgcacccaa aaggcatagt gccatagatc atcagctttg
      241 catttttaat tgtccctgaa cgggtcatgg tactcgctac agctgctgat gccgtagttc
      301 cagatgtaag tgtcccgtaa taggtctcat atgtctcagt attacgaatt cttccaaact
      361 ccaccaactg tgtagcactc tctacagctg ttttcagact ttgaacagtt gtattcaagt
      421 cagtagccgt gagggtttca ccacttgacc atgttttgat ggtacccgtc acactaaccg
      481 cgaataccgc cgtaccgagc acgacgagca aaccgattcc tctatagatt ccttctttta
      541 tatttcttaa catacgctac tcctccaatt atgatgactt gctatctctc aattgacctt
      601 gcaattgttc acggtcgctg accccaacac gcaggtggct gtgctgcccg acgttcccga
      661 ggtactattt tccgccgggc tttcgcttgc cgcagaaccg ccgcatgccg ctattgcgga
      721 gacaaaacca aacaaaatga cctttatcca tttccccatt tgatcctcca gaattaggga
      781 cgacaaatgc ccccgcttag caacctgtca acccaaaata cggcatatat gccgttcatg
      841 ttttccgttt ttatcggtac aaaccgcgat gaaagcgcgt ctatcggttg ctgatcaaaa
      901 agagtttgat cgttttttgg cgcagattgg tgagcgactg aaggcgcgcc gcgccgagtt
      961 aggtctgagg caagaagacc tcgatcttgc gccctgcccc atggacgtgc gcaagtacca
     1021 gcgcatcgag tatggccaga tgaactttac catgcagtcg ctctatgtgc tgagcaagaa
     1081 ccttaagctg gcacctgaaa aattactaac agggttgctt gaaccgagtt agactcagaa
     1141 tttgggccgc cgcacagacg caactggcgc tattcgccca gttacttagc gaaaaggcta
     1201 gtcaataaac gctgccggtg agacgttgaa gcgcttggct agcagtttga tttgccgcag
     1261 attaagagca cgctttttat gcaaaatctc agagagaaca ccctgactcg caatttcttt
     1321 caggtcagct tgtgtcaggt catgctcttt catcaaaatc tttaacatat caacagctga
     1381 aacgtcaggc accttcactt gttctgcctc atagatctca agcaggctac ccacagtctc
     1441 taacagtgga gcaagggggt gcttctcatc ttcaccgatt tcatcaacga gactatccat
     1501 aacgcggcag agtctattat attcacgctg gttggcaggt acggtaagca cttcaattct
     1561 tttgcggatt ttcagactct cccgccttag caccatctgc aagcggtggt tgcgggccct
     1621 gctgcgcggg ttgtgcgtgc gcccgctgta tatcaaacag ccgcggcatt tctgtcttaa
     1681 ccgcaagact cttgcccact gattctgcct cttgcgcgag cgccttgtcg aacagccact
     1741 cggtgactgc catgcgcgcg gcgagatcgc gcgtttcgat caacatctgc tcgcgcgcgt
     1801 gcgtgtattc gtggtatgcc ttgtcgtgca tcagctgaaa gccgcattta acgtcggcga
     1861 gaccgagaca gggtgaaggc cggcccgtgt acgtcgaatg cgcaaggcca gtggtgtgcc
     1921 cgatgcgcaa cttccaggtc gcatgcgcgg cgtgaaagtc gctcaggtag ttgcggatgt
     1981 attcgagaaa ttcggccggg gaaagcatcg cgttcgaaac atctttcgcc atggtcgtca
     2041 tgtcgccaaa aagcgaaccc tggtcgaagt ccttgatgat gacgactgcc agctggcggt
     2101 gtgcgcggcg ttctgcgcgc aacagcgaaa atatcttctc gcggctttcg ttcacccggc
     2161 ccctgagcga tggcatatcc tggtcaaaga cgacgatagc tgcgccgtgc ttcaattcac
     2221 gcagcagggc ctgcgatgaa tcgacagcca cgggattaaa accgtacaaa gaaaaaatcc
     2281 cactcgcctg cctgcgcagt gcttcgggta aaaatgccag caccacttcg ccaaaagccg
     2341 ccgacgcggt aagctgtatc aattgtgccg ctgtagtgag aaaatcttca aaagagatga
     2401 ggcaggtcag gccgtgatcg agcgcggtgc ggtaggcatg tgcggccgag ttgccgcagc
     2461 agaccagcag cgctgtaggc tcaaggctgt cgagctctgc agcgtggtga atgaaggttg
     2521 tgcgcgtgga ggtcaatgcc gggtgcagaa gctgcagcgc gtgaatgcgc tcgcggtcgg
     2581 cggcggatgc ctggaagaac acaatgcgtt caaaagcata cgccatctga tgtgacataa
     2641 tcctgacgcg ccgcacaggt cgtcaacgag gtttgcttcg ctggttcggc tgctactcag
     2701 cgcgaacgag gggtgcattt tgcttgccat tgtaacaaaa ttcgggc
//
LOCUS       DAOQJF010000403         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_25760_length_2746_cov_3.925307, whole genome shotgun sequence.
ACCESSION   DAOQJF010000403 DAOQJF010000000
VERSION     DAOQJF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25760_length_2746_cov_3.925307"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="PLF85_15705"
     CDS             complement(<1..1636)
                     /locus_tag="PLF85_15705"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNE21167.1"
                     /translation="MKSWYSLLAERNSPFAETIYQRFLDRLHEYTEGRLSPELQRQHA
                     LRYLLPSLHLLKSPFEFPAMERAVALLREFVAREGAKKLVIYGDRDADGVTSASVLTL
                     FCREQLKIGRESMTSFVPREEDKYGIAEAVAERIIAEKPDLLVTLDCGSSNKAELGRI
                     MAATGCKTIIIDHHFIPADPADYPAVEAFINPKLLPELEFVRDYCTAAITLRFVQAVL
                     YSYSREYNELIGIESPDQKVVLRNGVIVHGADFAAAARHYHVGGAAQGEERHNLLRIF
                     EHTARENADIRPLWLFYRKMPAAFSAAEIFNAVQSVSFKKSHAILAQYLPFAAIGLVA
                     DLMPVYADNRILIKEGLAAMTRQLNQLPMGLAALLKRLNLNSGVTEQDLGFNVCPVIN
                     AAGRMGNAALALAALTESDPLAATKAVSQLVQTNDARKAASQDSLDLLDQALDARSAA
                     AAVAVAVHDKFHRGISGLIASKIAERLAKPAIVLVPDGDCYRGSARAFKNENVHALID
                     RLKDFLLQYGGHRQAAGFSVSADNLAAFVDALFDAAP"
     gene            complement(1651..1974)
                     /locus_tag="PLF85_15710"
     CDS             complement(1651..1974)
                     /locus_tag="PLF85_15710"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE21168.1"
                     /translation="MAEKQETTVISDDMIFKGTVETDHPVLVEGKLSGSLIARGKVQI
                     AANAVVEANVSAREIDLEGTMQGNILHADLVTLHPSAKLNGDISCAQLEVQRGAKHSG
                     TTVMK"
     gene            complement(2031..>2746)
                     /locus_tag="PLF85_15715"
     CDS             complement(2031..>2746)
                     /locus_tag="PLF85_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNE21169.1"
                     /translation="LLAIIISYTVFIVRRSRRETRAVKAEYDEALSDERAEVKNVPVW
                     LSGLLVLVGLFILVLGARWLIEGSVVLARRFGVSETVIGLTIVAGGTSLPEVAASVMA
                     TIKGERDIAIGNIVGSCTFNIFSILGIAPLVSGGLPVAPEMLNVDFPVMLIAAIACFP
                     HFVAGLTFNRIEGVLFFISYAGYTTWLVLQATGSLMRPDYERVVMTVVLPILVPVVLL
                     VLWSKYRLSRSTPKNAKSS"
BASE COUNT          609 a          793 c          781 g          563 t
ORIGIN      
        1 caggtgcagc atcaaagagc gcatcaacaa aggcggcaag gttatcggcc gaaacagaaa
       61 aacctgctgc ctggcggtgc ccgccgtatt gcagaaggaa atccttcaga cggtcgatga
      121 gcgcgtgcac attttcgttc ttgaacgccc gcgccgagcc gcggtaacaa tcgccgtcgg
      181 gtaccagaac gatcgccggt tttgccagcc gctcggcaat cttcgaggca atcaaacctg
      241 aaattccccg gtggaatttg tcgtggactg ccacggcaac cgcggcagcc gcagagcgtg
      301 catcgagcgc ctggtccagc aggtcgagtg aatcctgcga ggctgccttg cgcgcatcgt
      361 tggtctgcac gagctggctc actgccttcg tcgcggcaag cgggtcactc tcggtcagcg
      421 cagcgagtgc cagcgctgca ttgcccatgc gccctgcagc attgatcacc gggcagacat
      481 taaagccgag atcctgctcg gtcacgcccg agttcaggtt caggcgcttc aagagcgccg
      541 caagccccat gggcaactgg ttgagctgcc gcgtcatggc agccagacct tctttgatga
      601 gtatgcggtt gtcggcgtag acaggcatga ggtcggcgac aagtccgatc gcggcaaacg
      661 gcaggtattg tgccaggatc gcgtgggatt tcttaaagct gactgactgg acggcgttga
      721 aaatttctgc cgccgagaat gcagccggca ttttgcggta aaacagccac agcgggcgga
      781 tatcggcatt ctcgcgcgcc gtgtgttcga aaatgcgcag caggttgtgg cgctcttcgc
      841 cctgcgctgc cccgcccacg tggtagtgtc gcgcagccgc cgcaaaatct gcgccgtgga
      901 ctataacgcc gttccggaga acgaccttct ggtcaggcga ctcgatacca atgagttcgt
      961 tgtattcgcg gctgtacgaa tagagcactg cctgcacaaa gcgcagcgtg atcgccgcag
     1021 tgcaatagtc gcgcacaaat tcgagttcgg gcaagagttt cgggttgatg aaggcttcca
     1081 ccgcagggta atccgcaggg tcggccggga taaagtggtg atcgatgatg atggtcttgc
     1141 agccggtagc cgccatgata cgccctaact ccgccttgtt cgacgaaccg cagtcaagag
     1201 ttaccagcag gtctggtttt tcggcgatga tacgttcggc aaccgcttcg gcgatgccgt
     1261 acttatcttc ttcgcgcggt acgaagcttg tcatcgattc acggccgatt ttcagctgct
     1321 cgcggcaaaa cagcgtcagc accgaggcgg atgtcactcc gtctgcgtcg cggtcgccgt
     1381 agataaccag ttttttcgcc ccttcgcgag caacaaattc gcgtagcaag gctaccgccc
     1441 gctccatcgc cggaaactca aacgggctct tcaacaaatg cagcgaaggc aagaggtaac
     1501 gcagcgcatg ctggcgctgc agctcgggac tcaaccggcc ttcggtatat tcatgaaggc
     1561 ggtcgagaaa acgctggtag atggtttctg cgaaagggga atttctttcg gctagcaggc
     1621 tataccacga cttcatgtgg taagcgccgg ttatttcatg actgtagtac cggagtgttt
     1681 ggccccgcgc tgcacttcaa gctgcgcgca gctgatgtcg ccgttgagtt ttgccgaggg
     1741 atgcagggtc acgagatcgg cgtgaagtat attaccctgc atagtgcctt cgaggtcaat
     1801 ttcccgggca ctgacatttg cctcgaccac ggcgttggct gcgatctgga ctttgccgcg
     1861 ggcaatcagg ctgccggaaa gtttaccttc caccaggacg gggtgatcgg tctcaacagt
     1921 acccttgaag atcatgtcgt ccgatataac ggtggtttcc tgtttttcag ccatgtgact
     1981 cctgcccaag tacggtaagg gccactttga cgaaaatttc tttcagccct tcaagaactt
     2041 tttgcattct ttggagttga acgactcaat ctgtacttgc tccagagcac cagcaaaacc
     2101 actggtacca gaatgggcag cactacggtc atcaccacgc gctcgtaatc ggggcgcata
     2161 agactgccag ttgcctgcag caccagccac gtggtgtagc ccgcgtaaga gatgaagaag
     2221 agcacacctt caatacggtt aaaggtcaac ccggcaacga aatgcgggaa acaggcgatc
     2281 gcggcaatca gcatgacagg aaaatcgaca ttcagcattt ccggtgcgac aggcaaaccg
     2341 ccggatacca gcggtgcaat accaagaatc gagaagatgt taaacgtgca gctgcctacg
     2401 atgttgccga tggcaatatc ccgctcaccc ttgatcgtcg ccatcaccga agcagcgact
     2461 tcgggcagag acgtgccacc ggcaacgatc gtgagcccga tcaccgtctc gctcaccccg
     2521 aagcggcggg cgagcactac agaaccttcg atcagccagc gcgccccgag cacgagaata
     2581 aacaggccga cgagcaccag taagcccgac agccaaacag gcacattctt cacctcggca
     2641 cgttcgtcgc tgagggcttc gtcgtattct gctttcaccg cgcgcgtttc gcgccggcta
     2701 cgcctgacga taaataccgt gtacgaaata attatcgcca gcaaca
//
LOCUS       DAOQJF010000404         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_25897_length_2737_cov_3.625280, whole genome shotgun sequence.
ACCESSION   DAOQJF010000404 DAOQJF010000000
VERSION     DAOQJF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25897_length_2737_cov_3.625280"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..632)
                     /locus_tag="PLF85_15720"
     CDS             complement(141..632)
                     /locus_tag="PLF85_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21170.1"
                     /translation="MTYEEILSLLLALIGIVGATLLIARMARSERRLRSLRLSTLDLR
                     TRSRAQQLNAILIAENAKLVAKGIDQGSILMQGGHRLISDVTFALLDLIPATRAHARV
                     VRETHNLVSTGLYGAFREINRQVTDRVAENLEKPKAAHKKASKRQKVRDRVKKRVAKK
                     SKR"
     gene            complement(636..998)
                     /locus_tag="PLF85_15725"
     CDS             complement(636..998)
                     /locus_tag="PLF85_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21171.1"
                     /translation="MNLLNATVVALFALCSGMALHYYFGLRKALRRAEAELAELRRLA
                     AAGDTARPAGETPLIRMLVEVKDPIALAHRESPLAKLASGTAPNLVTKKVYEQVVAQT
                     RQQLKDKNVDAEVSIIVI"
     gene            complement(1064..2356)
                     /locus_tag="PLF85_15730"
     CDS             complement(1064..2356)
                     /locus_tag="PLF85_15730"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21172.1"
                     /translation="MVAVFQRFWQWVSDFGIPEGISPDERKVHVSANQLMMVTLILIA
                     MMSLSLLIAWLLLLKINFVFYRYYFLGGLPAVLALVIGSGIYIYRGKTGRFYGPIVAF
                     AYLMSLLIASLTVIFGGVYGLEYWVLVVMGIGIFVLHKRKFLRRSGVASSMAVFIGLR
                     IWTYYVPPYMPVPHEGTRIFYVVFTGLALCVIMMLEFRFISQQSARAEDEFIAQRDIA
                     DRLLLNIMPQSVAEELKQRGKYEPRHYDSVTVIFTDFVGFTRLAAGMTPSELLTSLDD
                     CFGAFDRIVQKHGLEKLKTIGDSYMCAAGIPDPLSDHAERACRAASEFLQVISAKKAA
                     AQAAGKPYWDIRIGLHTGPLVAGIIGHHKFAYDIWGDTVNIASRMESSGEIGRINLSA
                     TTAKSLGSQFTVRSRGLQNAKSLGETEMFELVTGNEAT"
     gene            complement(2398..>2737)
                     /locus_tag="PLF85_15735"
     CDS             complement(2398..>2737)
                     /locus_tag="PLF85_15735"
                     /inference="COORDINATES: protein motif:HMM:NF027443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4833 domain-containing protein"
                     /protein_id="HNE21173.1"
                     /translation="PIHGYWQMHEKGPGVTEPITVFEQVAFGIRSQKVDGAGVRLTLA
                     ALPDREIGIEPEPGATGRYRARIQIAGQQAQLISFYAHAEPGFLMPKVKYIEIIGTID
                     GNEVRERINK"
BASE COUNT          510 a          777 c          883 g          567 t
ORIGIN      
        1 taatagtcgg cgacacgccg aatgacatcg cctgcgcgcg tgctgcacag gcgcagtgtg
       61 tcgcggtcgc cacggggctc tatggtctgg aagagctttc taccgccgat cgcgtgcttg
      121 ccgacctcac cgagtggtga tcagcgttta gattttttcg cgacgcgttt tttcacccgg
      181 tcgcgtactt tctgcctttt gctggccttc ttgtgagctg ctttcggttt ctcgagattt
      241 tccgcgacgc ggtcggtgac ctggcggtta atttccctga acgcaccgta gagaccggtg
      301 ctgacgaggt tgtgcgtctc gcgcacgacc cgcgcgtgcg cgcgtgttgc aggtatgagg
      361 tcgagcagcg caaacgtgac gtcgctgatc agccggtggc caccctgcat gagaatcgaa
      421 ccctggtcga tacccttcgc gacgagtttc gcgttctcgg cgatgagtat cgcattgagc
      481 tgctgtgcgc ggctgcgcgt gcgcaagtcg agggttgata gccgcagcga gcgcagccgg
      541 cgttctgaac gcgccatgcg cgcaatgagc agcgtcgcgc cgacgatgcc gatcaaggca
      601 agaagcagtg acagtatttc ttcgtatgtc atggttcaga tcacgatgat gctgacttcg
      661 gcgtcgacgt tcttgtcctt gagctgctgc cgggtctgtg cgaccacctg ctcatagacc
      721 ttcttggtga cgaggttggg ggctgtgccc gaggcgagtt tcgcaagcgg tgattcgcgg
      781 tgtgcgagcg cgatggggtc tttcacctcg acgagcatgc gaatgagcgg ggtttcacct
      841 gcaggccgcg cggtatcgcc tgccgcggca aggcggcgca attcggcaag ttcagcttcg
      901 gcgcggcgca atgccttgcg caggccgaag tagtagtgca gcgccatgcc gctgcagagc
      961 gcgaacaggg ccaccacggt ggcgttgagc aggttcatgg cacaccgggg ctgttacgtg
     1021 cggcgggcgt aaaacgattt tcgccagggt gaaggccgcg ggctcaggtg gcttcgttgc
     1081 cggtgacgag ctcgaacatt tcggtttcgc cgaggctctt ggcattctgg aggccgcgcg
     1141 agcgcacggt aaactggctg ccgagactct tcgccgttgt cgcagagagg ttgatgcggc
     1201 caatttcacc cgacgactcc atgcgcgaag cgatgttgac cgtatcgccc cagatgtcgt
     1261 aggcgaactt gtggtggccg atgatgcccg cgaccagggg ccccgtgtgc aggccaatgc
     1321 gaatatccca gtagggtttg cccgccgcct gcgccgctgc ctttttcgcg ctgatcacct
     1381 gtaagaactc gctcgcggcg cggcaggcgc gttcggcatg gtcgctcaac gggtcgggaa
     1441 ttcccgcggc gcacatgtag ctgtcgccga ttgtcttgag tttttcgaga ccatgcttct
     1501 gcacgatgcg gtcaaacgca ccgaagcagt cgtcgagcga ggtgagcagc tcgctgggcg
     1561 tcatgcccgc ggcaagccgc gtgaagccga cgaagtcggt gaagatcacc gtgaccgagt
     1621 cgtagtggcg cggctcatat ttgccgcgct gcttcagctc ttcggcgacg ctctgcggca
     1681 tgatgttgag cagcaggcgg tcggcgatat cgcgctgcgc gatgaactcg tcttcggcac
     1741 gcgcgctctg ctgtgaaata aaacggaact cgagcatcat gatcacgcac agtgccagcc
     1801 cggtgaaaac gacatagaag atgcgcgtgc cttcgtgcgg tacaggcatg taaggtggta
     1861 cgtagtatgt ccagatgcgc aggccaatga agacggccat ggatgaggcg acaccgctcc
     1921 tgcgcaaaaa cttgcgcttg tgcagcacga agataccaat gcccattacc acgagcaccc
     1981 agtattccag cccgtagacg ccgccgaaga taacggtgag tgaagcgatc agcagcgaca
     2041 tgaggtaagc gaacgcgacg atcgggccgt agaagcggcc ggtcttgccg cggtagatgt
     2101 agatgcccga accgatcacg agcgccagaa cagcgggcaa tcctccgaga aagtagtagc
     2161 ggtagaacac gaagttgatc ttcagcagca aaagccaggc gatgagcagc gacaggctca
     2221 tcatcgcgat gaggatgagt gtcaccatca tgagctggtt cgccgaaacg tgtactttgc
     2281 gctcgtcggg tgaaatgcct tcggggatgc cgaaatcgga aacccattgc cagaatctct
     2341 ggaacaccgc gaccatgggg cagcctgtgg ctcgacgggt atcgtcaagc cgttgaacta
     2401 cttgttaatg cgttcgcgta cttcgttgcc gtcgatggtg ccgatgatct cgatgtactt
     2461 caccttcggc atgagaaagc ccggctctgc gtgcgcgtag aacgagataa gctgtgcttg
     2521 ctggcccgcg atctgtatgc gcgccctgta gcgccccgtt gcgcccggct cgggttcgat
     2581 gccgatctcg cggtcgggca gtgcggcgag cgtcagtcgc acacctgcac catcgacctt
     2641 ttgcgagcgg atgccaaacg ccacctgctc gaaaacggta atgggttcgg tgacgccggg
     2701 gcctttttcg tgcatctgcc agtaaccgtg tatgggg
//
LOCUS       DAOQJF010000405         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26084_length_2726_cov_6.441408, whole genome shotgun sequence.
ACCESSION   DAOQJF010000405 DAOQJF010000000
VERSION     DAOQJF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26084_length_2726_cov_6.441408"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLF85_15740"
     CDS             <1..223
                     /locus_tag="PLF85_15740"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF020720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNE21174.1"
                     /translation="LLLPVDALLQGLPVVELDAAGRARFANGNPVPCLQAGLLGPCRV
                     YAARALLGLGRADSDGKLHPQRLVARAES"
     gene            complement(325..921)
                     /locus_tag="PLF85_15745"
     CDS             complement(325..921)
                     /locus_tag="PLF85_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE21175.1"
                     /translation="MTRTTYPALPTGRISPAGCFLSTSHNPTQVRIDSPALQVMTDLT
                     RLPAAIICRSAFVHEAHQAMIHRGVRMLFVANEDRQLEGIVTASDLLGERAMQAMSRY
                     ELTREELRVEHIMTTAAALETIELAMVTRAEVGHVIATLQTCGRQHAFVVDRNAAGRQ
                     IICGVFSATQIARHLGAPVHAGEIARTFAEIEAVIAGS"
     gene            1239..2429
                     /locus_tag="PLF85_15750"
     CDS             1239..2429
                     /locus_tag="PLF85_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21176.1"
                     /translation="MLTAFTSLILQISPAMYRWFKRFCLFFVCSCLLLSEMAQADWTE
                     VIRRMNTDTLVWRVNIVDSLGNPLPGATVWRMIPNGNQRKSLDLMQRLVRRYAFDQDF
                     VMNTTVHPSLMVSYANSEGVFVESDNVNYHKSNDVTTIYAVLKRGYLPTILSDVARKN
                     TTHEHTITLQPDPSAKVDQRMLQFDLIRAQAYMPAANAAERMQEDRRQLLLRTQQELR
                     SLAQALEQDKQYDLASAVYYNLAFLPSVDVVRQPNGSEVIVGYTNGYDEKKVQRKTDM
                     DKAQDLNQTNPNLIRKKMMRSFPGEDLGRLSPAQRQGYISLVEAQIRREPERVWPNVY
                     FVSYQYLDEGLYAEACSALNRAYQFEPTYCDDNQWRSHLRFHVSQAKKAGYPGEVCTI
                     RGIE"
BASE COUNT          586 a          780 c          778 g          582 t
ORIGIN      
        1 gctgctgcta cccgtcgatg cgctgctgca ggggttgccg gtcgtggaac tggatgcggc
       61 agggcgcgcc cgcttcgcca acggcaatcc cgtgccctgc ctgcaggccg gcctgctcgg
      121 cccctgccgg gtctatgccg cgcgggcgct gcttggcctt ggccgtgccg acagcgacgg
      181 gaagctgcac ccgcagcgcc tcgttgcccg cgccgagagc tgaagaaaaa ccgtcacacc
      241 ggcgaaagcc ggtgtccagt acgttcacaa ttctggattc cggcttccgc cggaatgacg
      301 gttgttggtg ctcccgtacc cgccctagct ccccgcgatc accgcctcga tctcggcaaa
      361 ggtacgcgca atctccccgg catgcaccgg cgcacccagg tgccgggcga tctgtgtcgc
      421 cgagaaaacg ccgcagatga tctgccgccc ggcggcattg cggtcgacca cgaaggcatg
      481 ctgccgcccg caggtctgca gcgtcgcgat cacgtggccg acttcggcac gggtcaccat
      541 cgccagctcg atcgtctcga gggctgctgc cgtcgtcatg atgtgctcga cacgcaactc
      601 ctcgcgcgtc agttcgtaac ggctcatcgc ctgcatcgcc cgttcgccga gcaggtcgct
      661 ggcggtcacg atcccctcca gctgccggtc ctcgttcgcc acgaacagca tgcgcacgcc
      721 gcggtggatc atcgcctgat gcgcctcgtg cacgaaggcg ctgcggcaga tgatcgccgc
      781 cggcagtcgg gtcaggtcgg tcatcacctg cagtgccggc gaatcgatgc gcacttgcgt
      841 cgggttgtgg ctggtggaaa ggaagcagcc cgcgggggaa atcctgccgg tcgggagggc
      901 ggggtaggtg gttcgcgtca tgatctgtct ccttgggtgg tcgttcagaa acagcgggga
      961 tgcaaggctg tgactgggga cagggaggcg aagttcctgc agctggcggt gactcctagc
     1021 cggaagtcat atatcccgga ctggcggtgg cggatacact ctcatctttc tgccaggtag
     1081 tcacaagatc ggaaaacgga atgatgctga cccctttagc tggtcacaag atcggaaaac
     1141 ggaatgatgc tgaccccttt agctgtagct ttcccatcac acacctccac agacattttg
     1201 cctactttca aatctgtccg tggaacccgg gatacctcat gctgaccgca ttcacttctt
     1261 taattcttca gataagtcct gcgatgtatc gttggtttaa acgcttttgt ctctttttcg
     1321 tttgctcgtg cctccttctt tcagagatgg cgcaagccga ttggacggaa gtgattagaa
     1381 gaatgaatac cgataccttg gtttggcgcg tgaacatcgt tgactcgctg ggaaatccgc
     1441 tgcctggtgc gaccgtttgg cgaatgattc ccaacggcaa tcagcggaaa agtctagacc
     1501 tgatgcaacg gttggtgcgg cgttatgcat tcgaccaaga ctttgtgatg aatacaacgg
     1561 ttcatccaag cctgatggtt agctacgcga atagcgaagg ggtttttgtt gagtccgata
     1621 acgtcaacta ccacaaaagt aatgacgtaa cgacgatcta tgctgtactg aagcgcgggt
     1681 atttgccgac gattctgagt gacgttgcgc gcaagaacac cacgcacgaa catacaatta
     1741 ccttgcagcc ggatccctca gccaaagttg atcaacgcat gttgcagttc gatctcattc
     1801 gggcgcaggc ctatatgccg gcagcgaacg cggcagagcg aatgcaggaa gatcgccggc
     1861 aactcctgct gcgcacacag caagagttgc gcagcctggc tcaggctcta gaacaggaca
     1921 aacaatatga tctggcgagt gcggtgtatt acaacctcgc atttctgcct tccgtcgatg
     1981 ttgttcggca acccaatggc agcgaagtta tcgtcggcta tacaaatggt tacgacgaaa
     2041 agaaggtgca gcgcaagacg gatatggata aagcgcagga tttgaatcaa acaaacccca
     2101 atttaatccg gaaaaaaatg atgcgctcat tccccggcga ggatttgggc cgtctatcac
     2161 cagcgcaaag gcagggctac atcagcttgg ttgaagcaca aattcgacgc gagccggaac
     2221 gcgtatggcc aaacgtatat tttgtgagtt atcaatatct cgatgaaggc ctctatgctg
     2281 aggcgtgctc agcactgaat cgtgcctatc agtttgaacc gacctattgt gacgataatc
     2341 agtggcgatc tcatctgcgt tttcacgtat cgcaggcaaa gaaagctgga tatccgggcg
     2401 aggtatgcac aattcgcgga attgaataat tgagtaatca ccaaacgggt cagcatcgat
     2461 tagtttttcc ccccaacacc aatcaagccc gaaaaaaatg cgctcgccaa gctgacgagc
     2521 gcattcttgc cggatcaggt gttaccgagg ttcagtcagc cttcccgcag cactgcttgt
     2581 acttcttccc actgccgcag gggcaggggt cgttgcggcc aaccttgccg ccgtcgcggc
     2641 ggacggtgcc ggcaccgcgc ttggcctgcc agaacaggaa aatctccggg cactaaaggt
     2701 gtcagcgtca attaactttc tgcaca
//
LOCUS       DAOQJF010000406         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26086_length_2726_cov_4.372520, whole genome shotgun sequence.
ACCESSION   DAOQJF010000406 DAOQJF010000000
VERSION     DAOQJF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26086_length_2726_cov_4.372520"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PLF85_15755"
     CDS             complement(<1..1260)
                     /locus_tag="PLF85_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21177.1"
                     /translation="MKARLSILAVVAMLGTACGGVKSQIRENRAQDAGRDAVAVYGEK
                     TEQPNDYALLMEELQSQNRDCTVKTGGKTNWVLCSAYSHEHMQGIAALSGALSGPDDL
                     VWTVTSDEKLRAFLTRIKQVRARDQSLKYREALADLEQLSGLTASGYYARYADSLRER
                     AERPEKNLNRVLTALEKPDTAVISDAEAAEAIREAFLVEPSLTANEKYRKRYAQIVQA
                     LPQKITCTVKLTEPPGKVYISTGYELPIMAQANCDKAQARGNLALRLDSAGPVRFGSS
                     EIRNTIVELADLKGEITVSELSAIKEENKVQFSGVPHFAETFEALQPLGAKPKLEFAL
                     TPAEYTLQNVPAFFSADYERLASGVQPSTPWKTLKGKETEATLGMNGQFTMGGSRNGV
                     PYDMVFGHPNGKHSDGIWSSFITISVNE"
     gene            complement(1257..2318)
                     /locus_tag="PLF85_15760"
     CDS             complement(1257..2318)
                     /locus_tag="PLF85_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21178.1"
                     /translation="MRAFLVLSFFCFAGLVAQSSDIPLSTDKVYIGKPEGVRNETPEE
                     DIIKARQLKAEKRRSLELTARLSEARSGARSVQLADKSYCANSRAQFAIAAEDDLSGL
                     ARFHYKIDDAEWMSYTGPVSISAEGVHYLKWESFDKVGNREDQQTLKVRIDDTAPALT
                     VTPHGRFVVRKNGFIAAPGFRFVVSAHDHDCGLQSMYANINNEGWHALKGGIDFEKPG
                     LHQAQFMADDILGNRSNVTAHTVEIDGTAPVTRIEVTPRPIQAESDLVCKPNTVVTIS
                     AADRESAVERIEYRYSPNAAWAGSMDTTMPVARSENFYIEARSRDIVGNTESKPAIFK
                     CRTSGSPPRTVITPVGEKK"
     gene            complement(2459..>2726)
                     /locus_tag="PLF85_15765"
     CDS             complement(2459..>2726)
                     /locus_tag="PLF85_15765"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNE21179.1"
                     /translation="TLKKKLDADIRPYKILGACNPPFALKSLQAEDYIGLMLPCNVLL
                     QQRDGGVEVSAIDPVASMMAVKNPQLEATAGEVREKLRRVIAAL"
BASE COUNT          547 a          749 c          745 g          685 t
ORIGIN      
        1 ttcattcacg cttattgtga tgaacgacga ccaaatgccg tcagaatgtt tgccattggg
       61 atgtccaaag accatgtcgt aaggcacgcc gttgcgactg ccgcccatgg tgaactggcc
      121 attcatgccg agggtggcct cggtctcttt gcccttgagg gttttccagg gtgtcgaagg
      181 ctgaaccccg ctcgcgaggc gctcgtaatc tgcggaaaaa aatgccggca cgttttgtag
      241 cgtgtattca gcaggcgtga gagcaaattc gagtttgggt tttgcgccaa gcggctgaag
      301 cgcctcgaaa gtttcggcaa aatgcggcac gcccgaaaac tgtactttat tttcctcttt
      361 aatcgccgaa agttcagaaa cagttatctc ccctttcaga tcagcaagtt caacaatggt
      421 atttcttatc tctgagctgc cgaaacggac ggggccggca gaatcgaggc gcagcgccag
      481 attgcctctt gcctgcgcct tgtcgcagtt tgcctgagcc ataatcggca attcataacc
      541 ggtactgatg tagaccttgc cgggcggttc ggtgagtttt acagtgcagg taatcttttg
      601 tggcagagct tgtacaatct gagcatagcg tttgcggtac ttctcattgg cggtcagcga
      661 cggttcgaca agaaacgctt cacgtattgc ctcagcggct tcggcgtcgc ttatcaccgc
      721 ggtatcgggt ttttcgagag cggtgagcac ccggttgagg tttttctcgg ggcgttcagc
      781 tctttcgcgc aatgaatctg cgtagcgcgc ataatagccg cttgccgtga ggcccgaaag
      841 ctgctcgaga tcagccagcg cttcccggta tttcagagat tgatcgcgcg cacgcacctg
      901 cttgatacgc gtcagaaatg cccgcaattt ttcgtcgctg gtgaccgtcc aaaccaaatc
      961 gtcagggccg gataacgcgc ccgaaagggc tgcaattccc tgcatatgtt catgcgaata
     1021 tgctgaacac aatacccagt tcgttttccc gccagtcttc actgtgcagt cgcggttttg
     1081 gctttggagt tcttccatca gcagggcata atcattcggt tgctcggttt tttccccgta
     1141 aacggcaacg gcgtccctgc ccgcgtcttg agcgcggttt tcgcggatct ggctcttcac
     1201 tccgccgcag gctgtaccca gcatggcgac aaccgccaat atggagaggc gcgccttcat
     1261 tttttctctc ctacgggagt aatcaccgtt ctgggggggc tgcccgaggt gcggcattta
     1321 aatattgcgg gtttgctttc ggtattgcca acgatatcgc gtgagcgcgc ttcgatgtag
     1381 aaattctctg atcttgcgac tggcatggtg gtatccatgc tgccggccca cgccgcgttg
     1441 ggcgagtagc ggtattcaat tctctcgaca gccgattcgc ggtcggccgc cgagatagtc
     1501 actaccgtgt tgggcttaca gaccagatcg ctttcggctt gaatgggtcg tggtgtcact
     1561 tcaatgcggg taaccggcgc cgttccgtcg atttcgactg tgtgcgcagt aacattgctg
     1621 cggttaccca gaatatcgtc tgccatgaat tgagcctggt gcaagccggg cttctcgaaa
     1681 tcaataccgc ctttcagtgc gtgccagcct tcgttgttga tattagcgta catgctctgc
     1741 aatccacagt catgatcgtg tgcgctaact acgaaacgaa accccggcgc ggcaatgaag
     1801 ccattcttac ggacgacaaa tctgccgtgc ggggtgacgg ttagcgcggg cgccgtatca
     1861 tcaatacgca ccttgagggt ttgctggtct tccctgttgc caactttatc gaagctttcc
     1921 catttcagat agtgcacacc ttctgcgctg atcgaaaccg ggccggtata actcatccac
     1981 tctgcatcgt cgatcttgta gtgaaagcgt gcgagaccgc tcaggtcgtc ttcggcagca
     2041 atggcaaact gcgcgcgcga attcgcgcag taagatttat cagcgagttg cacagagcgc
     2101 gctccgctgc gcgcctcgga caatctggcg gtgagttcga ggctcctgcg tttttcagcc
     2161 tttagttgcc gggctttgat gatatcttct tcaggcgttt cattgcgaac gccctcgggt
     2221 ttgccgatgt agaccttgtc tgtggaaagg gggatgtcac tgctctgcgc gacaaggcca
     2281 gcaaagcaga aaaaggacag aaccaggaat gcccgcatga acctgacgca ttgccttgag
     2341 cgtctgcgtc aaggagtttc agttgcctgg gccagtgaga ttcgatgcgc caattgcttc
     2401 cagttccgga agtcaataaa cgccagagcc ctgaaaaatc ccgctcttac acaattgatt
     2461 acaacgccgc gatcacgcgc ctcagtttct cgcgcacttc gccggcagtc gcctcgagct
     2521 gcggattctt caccgccatc atcgaggcca ccgggtctat cgctgagacc tcgacaccgc
     2581 cgtcgcgttg ctgcagcagc acattgcacg gtagcatcag cccgatatag tcctcagcct
     2641 gcagtgattt taaggcaaag ggcgggttgc acgcgcctaa gatcttgtac gggcgaatat
     2701 cggcatccag tttcttcttt aatgtg
//
LOCUS       DAOQJF010000407         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26187_length_2720_cov_5.136210, whole genome shotgun sequence.
ACCESSION   DAOQJF010000407 DAOQJF010000000
VERSION     DAOQJF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26187_length_2720_cov_5.136210"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLF85_15770"
     CDS             <1..495
                     /locus_tag="PLF85_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE21180.1"
                     /translation="PRQAAQIDRDPAFGPDGNLYPWGNETADCSRAIIQIGEGKKGIK
                     GCFPKRLEPEWHMHTAEVGTRPAGAYGLYDMAGNVHEWVNDWYAKSYAQCGEACAGKD
                     PKGPCGGAEKCPGYTMRSVRGGSWWWTADYARGSKRRANVPGNMPMADYHHFGFRCAK
                     DASP"
     gene            645..1172
                     /locus_tag="PLF85_15775"
     CDS             645..1172
                     /locus_tag="PLF85_15775"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21181.1"
                     /translation="MNLTEVKEKQIRDRVLATLPAELSLHKLAFFHSRDNMRIVVELD
                     KPSDKYGSVNIGECENYSRALRDNLDELEKTTAINLNYSLEVSSAGAERELATIEEVR
                     RFMALPLQASYESAGKVLTEVLQVLEIEGEKMECRLADCKANRKKFGPAKLRKQTPFH
                     IEWREIKKIRLHLDI"
     gene            1191..2585
                     /gene="nusA"
                     /locus_tag="PLF85_15780"
     CDS             1191..2585
                     /gene="nusA"
                     /locus_tag="PLF85_15780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNE21182.1"
                     /translation="MARAKKAVAEKKRSFFEDIQQVTADKGFDRQEILEVVEAGLIAA
                     YKRKYRTTDNVSVVMDKDKDDVYVIARRVVVDNIVLPGMQILLEDAKKIKPDAALGEE
                     IDVVEYPLEFGRIAAQTAMQIVSQRLRQLEQNKIREAYEDKIGEIMNAYILRKRGDTV
                     YVDLGKVEAIMPVKHQIPKERYRVEDKIKVLLHSIEQDKGGGPLRVIVSRADRMFVQR
                     LFEKEVPEIFEKIVTIKTIGRIPGERSKVVVASERSNVDPVGACVGVRGVRIQAIVRE
                     LGTERIDIVEWASDPREFITNALTPAAPSLVKVDQNAREALAVVPDKDLAIAIGREGT
                     NVKIASYVTGYKIDVKSESQFSAEMASPEARKRLDEIFDVTPAEEAPVEEEGTPLSAL
                     PGLAPRIIRILQENNIKYIEDLVSMGEEDLIHIEGIGRATAKRIMEIISENVEFEAGE
                     EEAEGQELDADAKA"
BASE COUNT          719 a          718 c          768 g          515 t
ORIGIN      
        1 ccccggcagg ctgcccagat cgatcgcgac ccggcgttcg gacccgacgg caacctgtac
       61 ccctggggca acgaaacagc cgactgctca cgtgcgatca ttcagatcgg cgaaggcaaa
      121 aaaggtatca agggttgttt cccgaagcgg ctcgaacctg aatggcatat gcataccgcc
      181 gaagtgggca cccgcccggc gggagcttac gggctctatg acatggcggg caacgtgcac
      241 gagtgggtga atgactggta tgcgaaaagt tacgcgcagt gcggcgaggc ctgcgccggt
      301 aaagacccca aggggccgtg cggcggcgcg gagaaatgcc ccggctacac gatgcgctcg
      361 gttcgcggcg gttcgtggtg gtggacggct gactatgcac gcggttccaa gcggcgcgcg
      421 aatgttcccg gcaacatgcc catggcggat taccaccact ttggcttccg ctgcgctaaa
      481 gacgcttcac cttagccctt ctgcaggcag tttagcggcc tgctaatggt ttgacaccct
      541 gtttaaagga cagtctgtgg actgtggaaa agtggcgtaa gccgcttttt ttttgaggcc
      601 ctggatttac gcttttaccg gtaaaccacg ccgcacaaac cagaatgaat ctaaccgagg
      661 taaaggaaaa gcagatacgc gatcgcgtgc tggcaacgct gccggcggag ctatcgctgc
      721 acaaactcgc tttttttcac agccgcgaca acatgcggat tgtcgtcgag ctcgataaac
      781 cctcagacaa gtacggctcg gttaatatcg gtgaatgcga gaactattca cgcgcgctgc
      841 gcgacaacct cgatgaactg gaaaagacaa ccgcaatcaa cctcaactac tcgcttgagg
      901 tttcttctgc cggcgccgaa cgcgaactgg caacgatcga agaagtgcgg cgcttcatgg
      961 ccctgccgct gcaggcaagc tacgagagtg ctggcaaagt actgactgaa gtcctgcagg
     1021 tgctggagat cgaaggcgaa aaaatggaat gcaggctggc cgactgcaag gcaaaccgca
     1081 agaagtttgg cccggccaaa ctgcgcaagc aaacaccttt tcatattgag tggcgtgaaa
     1141 taaaaaaaat caggttgcac ctggacatct gagacttaac gggagaaaac atggcaagag
     1201 caaaaaaagc agtggcagag aagaagcgct ccttcttcga agatatccag caggtgacag
     1261 ccgacaaggg ttttgatcgc caggaaattc tcgaagtcgt tgaagcaggc cttatcgccg
     1321 cgtacaagcg caaataccgc acgactgata acgtcagcgt cgtcatggac aaagacaaag
     1381 atgatgtcta cgtcattgcc cgccgtgtcg ttgtcgataa catcgtactg ccaggcatgc
     1441 agatactgct cgaagacgca aagaaaatca aacccgatgc agcacttggt gaagaaatcg
     1501 atgtagtcga atacccgctg gaattcggcc gcatcgccgc gcaaaccgca atgcagatcg
     1561 tatcgcagcg cctgcgccag ctcgagcaga acaagatacg cgaagcttac gaagacaaga
     1621 ttggcgaaat catgaacgcg tacattctgc gcaaacgcgg cgacacagtc tacgttgacc
     1681 ttggcaaggt tgaagccatc atgccggtta agcaccagat tccaaaagag cgctatcgcg
     1741 ttgaagacaa gattaaagta ctgctccact cgatcgagca ggacaagggc ggtggcccac
     1801 tgcgcgttat tgtgtcgcgc gccgatcgta tgttcgtcca gaggctgttc gaaaaagaag
     1861 tccccgaaat ttttgagaag atcgttacga tcaaaaccat tggacgcatt cccggtgaac
     1921 gatcaaaggt agttgttgcg tctgaacgct cgaatgtcga cccggtgggt gcctgcgtag
     1981 gcgttcgcgg cgtccgtatt caggcaattg tgcgtgaact cggcactgag cgcattgaca
     2041 ttgttgagtg ggcgtctgac ccgcgcgaat ttatcaccaa cgccctgaca ccggcggcac
     2101 ccagcctcgt gaaagttgac cagaacgcgc gcgaagcact tgccgttgtg ccggataaag
     2161 acctggccat tgctattggc cgggaaggta cgaacgtaaa aattgcgtcg tacgtcaccg
     2221 ggtataagat cgacgtaaaa tccgaatcac agttctcggc cgaaatggcc tcgcccgagg
     2281 cccgcaagcg cctcgatgaa attttcgatg ttaccccggc cgaagaagcg ccggttgaag
     2341 aggaaggcac cccgctgtct gccctgcctg gcctcgcccc gcggattatc cgcattctgc
     2401 aggagaacaa catcaagtac atcgaagacc tcgtatcgat gggcgaagag gacctgattc
     2461 atatcgaagg tatcggccgg gcgacagcta agcggatcat ggagattatc tcagagaacg
     2521 tcgagttcga agcgggcgaa gaagaggcag agggtcaaga gcttgacgct gacgctaaag
     2581 cctgataaga gactatagat taaagaacac atagcgcgca tggcagaaaa aattaaactc
     2641 actcctaaaa agatcgtcct gaaaggaagt tcttccaagg actcaatcgc gggaaagatc
     2701 agcgcgcaaa aggttcaaca
//
LOCUS       DAOQJF010000408         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26287_length_2714_cov_4.424220, whole genome shotgun sequence.
ACCESSION   DAOQJF010000408 DAOQJF010000000
VERSION     DAOQJF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26287_length_2714_cov_4.424220"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLF85_15785"
     CDS             <1..835
                     /locus_tag="PLF85_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNE21183.1"
                     /translation="DFSMITSPVSLLTIFSIFFVASLESLLSASAVDKLDNEKRVSNL
                     NKEIIANGAANLLLGAIGGLPIIAEIVRSSANVSNGAKTQWSNFFHGLFLALFLFLIP
                     GVIHLIPNAALAGILILVGFRLARPSEFKHAFDAGIEQLAIFLTTLVVTLVEDLLVGI
                     AAGLIVKLLILVLRGVKLREVFSLRATVQDSGSEIYVKPEGSIIFTNLFSLTDRISAF
                     EKSKHLTIDFASVHYVDHTSMAALVTLRQEAEAEGRALTMLNIDLLKPYSSHPAAARR
                     A"
     gene            881..2548
                     /locus_tag="PLF85_15790"
     CDS             881..2548
                     /locus_tag="PLF85_15790"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNE21184.1"
                     /translation="MLTVSLHAQQAAAAESVKLPAEAPAAAASGKAGGNAFASPYRDS
                     GLPADALRPMLLDAALAQQVKNSQQLWIGDIMRIGLYSRVRGEFRDNLNFSASNPEKV
                     NRFSQQSQVFFFVNPARDTELKITLQDARLWGGDGGVSAGDDRAAFFTNGDNTSGKRN
                     SFDVREAYVQFRNLGAKGLGLQLGRQILAYGDGRMIGSANWTFGGLSYDGVLLKYDAD
                     AISSHLFAIKGTSSVTNNMPNGGVSNSPVPDGDSYMAGWYNSLKHYLAVLDIYAIGMF
                     RNAAVDSVCSVAGATCTPDIVVATAGRQRSNLYTFGARLTNRTEANRLPAGSKWDYTL
                     EAAIQAGNAPDVLFTDASGNTSTNGRRYAGQLFFAQTGYKVLDDLRVGVQAYYSPGTR
                     DRTGSTIDTFQTLPGPRFGGFPYLNNFNGISENMGMKNTFTPSVSLLYESPKWGDFVV
                     SYFYEMKATPQDAWYGISGLANSGTAAANSSGRISTESATNASGANLGSLLYHEVNLV
                     WMQRFSSWLSIWVGAGYLHAGNAIAIARGNDFKADAFMAFIQIQGAL"
     gene            complement(2604..>2714)
                     /locus_tag="PLF85_15795"
     CDS             complement(2604..>2714)
                     /locus_tag="PLF85_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNE21185.1"
                     /translation="ERAAVGILPGMVRISVGLENANDVLADIEQALKKSA"
BASE COUNT          576 a          747 c          739 g          652 t
ORIGIN      
        1 ggatttcagc atgatcacct caccagtatc cctgttaacc atattctcga tcttctttgt
       61 cgcgagcctt gaatctctgc ttagcgcaag tgctgtcgac aagctcgaca acgaaaaacg
      121 ggtcagtaac ctgaacaaag aaattatcgc gaacggggct gcgaacctgc tcctgggcgc
      181 aattggcggc ctgcccataa ttgccgagat tgtccgctcg tctgctaacg tgtctaacgg
      241 tgcaaagacg caatggtcaa acttcttcca cggtcttttt ctggcgctgt ttttgttcct
      301 gattccgggc gttattcacc tgataccaaa tgcagcgctc gctggtattt tgattcttgt
      361 cggcttccgt ctggcgcggc catcggagtt taaacatgcc tttgatgcgg ggatcgaaca
      421 gctggcaatc tttctgacaa ccctggtcgt cacgctggtt gaagacctcc tggtgggtat
      481 tgcggcaggt ctcatcgtca agcttctgat acttgtgctc cggggtgtaa aattgcgtga
      541 agtctttagc ctgcgggcaa ccgtccagga ttctggttcg gagatttacg taaagccgga
      601 aggttcgatc atattcacca atctcttttc gctgacagat agaatcagcg cctttgagaa
      661 gagtaagcat ctcacaattg attttgccag tgtacattac gtcgaccaca cctcgatggc
      721 cgccctggta acgctccggc aggaagctga ggcagagggg cgggcactga cgatgctgaa
      781 tattgatctc ctcaaaccct actcgagtca tcctgcggct gcgcggaggg cttaaatgcg
      841 ctgccgtttt cctctgcgga cattgcttac ctcagcggca gtgctgacag tttccctgca
      901 tgcacagcag gctgcagcag ctgaaagcgt aaagctgcct gcagaggccc ctgcagctgc
      961 agccagtgga aaggcaggag gcaacgcttt tgccagtcca taccgtgata gtggtctccc
     1021 ggctgacgca ctgcggccga tgctactcga tgcagcgttg gcacagcagg taaagaatag
     1081 ccagcagctg tggatcggcg atatcatgcg catcggtctc tacagccgtg tgcgcggcga
     1141 gttcagggat aacctgaact tcagcgcctc caatcccgaa aaggtcaacc gtttcagcca
     1201 gcaatcccag gtcttttttt tcgttaaccc cgcacgcgat accgaactca agattaccct
     1261 gcaggatgca cggctatggg gcggagacgg tggcgtgagc gcaggcgatg accgcgctgc
     1321 ctttttcacg aatggtgaca atacgagtgg caaacgcaac agttttgacg tgcgcgaggc
     1381 atacgtacaa ttccggaacc tcggcgcaaa ggggctaggg ctgcagctcg gccggcagat
     1441 actcgcatac ggcgacggtc gcatgatagg tagcgcgaac tggactttcg gcgggttgtc
     1501 ttacgatggc gtgctgctca agtacgatgc ggatgcgatt tccagccacc tgttcgcaat
     1561 caagggtacc tcgtctgtta ccaacaatat gccgaacggc ggagtcagca attcacccgt
     1621 cccggacggt gattcgtaca tggcgggttg gtataactcg ctcaagcact atcttgccgt
     1681 gctcgatata tacgcgatcg gcatgttccg caatgctgcg gtcgattcgg tgtgctcggt
     1741 tgcgggagca acctgtacac cggacatcgt tgtagcaact gccgggcggc agcgttcaaa
     1801 cctctacacc tttggcgcac gcctcaccaa ccgcaccgag gcgaacaggt taccggcagg
     1861 cagcaaatgg gattataccc tcgaagcagc catccaggca ggcaatgcac cggacgtcct
     1921 tttcacggat gcctcgggca atacgtcgac caatggtcgg cgctacgcgg gccagctttt
     1981 ctttgcccag actggttaca aggtgcttga tgacctgcgt gtgggagttc aggcgtacta
     2041 ttctcccggt acccgcgaca gaaccggttc gacaatcgat acgtttcaga ccctgccggg
     2101 gccacgcttt ggcggttttc cttacctgaa caacttcaac ggcatttcgg aaaatatggg
     2161 gatgaaaaac acttttactc cctcggtttc gctgctctat gaaagcccga agtggggtga
     2221 ttttgtcgtc agctatttct atgagatgaa agccacaccg caggatgcat ggtatggcat
     2281 cagcgggctg gccaactcgg gaacagccgc tgcgaattcg tcaggcagaa tttcaaccga
     2341 gagcgccacg aatgcttcgg gcgcgaatct gggttcgtta ctctaccacg aggtcaacct
     2401 ggtatggatg cagcgttttt ccagctggct ttcgatctgg gtcggtgcgg gttatttgca
     2461 cgcaggcaat gccattgcca ttgcgcgcgg caatgatttt aaggccgacg ctttcatggc
     2521 atttatccag atacaaggcg cgctctagtt cttctcttct actcctcact ctaaccggag
     2581 agtcgtatga ctctccggtt tttttatgcc gatttcttca gtgcctgctc aatgtcggcg
     2641 agcacgtcgt ttgcattttc cagtcccacg gagatacgca ccatgccagg cagaatgcct
     2701 accgctgcac gttc
//
LOCUS       DAOQJF010000409         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26385_length_2709_cov_5.551243, whole genome shotgun sequence.
ACCESSION   DAOQJF010000409 DAOQJF010000000
VERSION     DAOQJF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26385_length_2709_cov_5.551243"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /gene="typA"
                     /locus_tag="PLF85_15800"
     CDS             <1..1799
                     /gene="typA"
                     /locus_tag="PLF85_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNE21186.1"
                     /translation="AIIAHVDHGKTTLVDKILGFCSTLEAKESTERVMDSNDLERERG
                     ITILAKNTAVDYEGTRINIVDTPGHSDFGGEVERVLRMVDTCLLLVDAMEGPMPQTRF
                     VLKKALELGHKIILVINKVDKPNCNPQDVINQVFDLFVELNASDEQTDFPVVYASAKN
                     NYAFLEWADFEKPESKTTLKPLLDTIMTHARDGAGDPAAPFAFQVTNLDYNDYLGRIC
                     IGRVFSGTVKVGDQVVLRGENEKGEPTVSKHKITKLFDFLGLKRIDVTEASCPNIIAI
                     AGIEEMTIGDTLCHEAYADALPRIKIEPPTVSMQFSVNDSPFAGKEGKWVTSRNIRER
                     LERELKTNLALRVEDLGDSFKVAGRGELQLAILIENMRREGFELGVSKPQVIFKEEGG
                     QKLEPFEEIVMDMPEQYSGGVIQELNRRKGMMLIMETLSTGFVRVKYEMPTRGLIGFR
                     SFFLTETRGEGSMSGLFLEYRPYAGDFSGRTRGAIVSMESGATNAYGLFNIQDRGELF
                     VGAQTPVYVGMIIGLHAKDNDLDVNPIREKKLTNVRASGTDEALRLVPPRKLSLEQCL
                     DFLEDDEALEVTPTSLRLRKKVLNPSMRKRSA"
     gene            1806..2660
                     /locus_tag="PLF85_15805"
     CDS             1806..2660
                     /locus_tag="PLF85_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNE21187.1"
                     /translation="MFEYFSPQNLNALWQRLTSGHAFASTAMVVGVLLLVRLIVLRIA
                     YRNIQDKFLRLRYRRTVSYIISFIAIFLLLPIWLPSIRSIATFLGIFGAGFLIVTRDL
                     WVCVVGWAYIMIRRPFIIGDRIQIGNIAGDVVDIRLMETSVMEVSQAEGGQSTGRVVF
                     FPNAKIFTESFATTGANVGHIFQELQVHLSNRADWKTAANLLEKIAHRRYEEALAAKE
                     QLQINSEVDMISSYREPRVLAQMSGNHIVLKLQYMAPTGQGVNMNDHIWRDFLSAAQK
                     NPRIRFSE"
BASE COUNT          636 a          783 c          700 g          590 t
ORIGIN      
        1 tcgccattat tgcccacgtc gaccacggca aaacaactct cgtcgacaag attctcggct
       61 tttgctcaac gctcgaagct aaagaaagca ctgaacgcgt tatggactcg aacgacctcg
      121 agcgtgaacg cggtattacg attctcgcga agaacaccgc ggtcgattac gaaggcaccc
      181 gcatcaatat cgtcgataca ccgggccact cagacttcgg tggcgaagtt gagcgtgtac
      241 tccgcatggt cgacacctgc ctgttgctcg tcgacgcgat ggaaggcccg atgcctcaga
      301 cccgtttcgt gctcaagaaa gcgctcgaac tcgggcacaa gattatcctc gtcattaaca
      361 aagtcgataa acccaactgt aacccacagg atgtcatcaa ccaggtcttt gacctctttg
      421 ttgaactgaa cgcctctgac gagcagacgg acttcccggt tgtttatgcc agcgcgaaaa
      481 ataactacgc ctttctcgag tgggcagact tcgaaaaacc cgaatccaaa acgacactca
      541 agccgctgct cgacaccatc atgacgcatg cgcgcgacgg cgcaggcgac cccgcagcgc
      601 cttttgcctt ccaggtaacc aacctcgact acaacgacta cctcggccgt atctgcattg
      661 gccgcgtctt cagcggtacg gtaaaagtcg gagaccaggt tgttctgcgc ggtgagaatg
      721 aaaagggcga accgacagta tcaaagcaca agattaccaa gctctttgac tttctcggcc
      781 tcaagcgtat tgatgtgacc gaagcttcat gcccgaatat catcgcgatt gccggcattg
      841 aagaaatgac aatcggcgat acgctctgcc acgaagctta tgccgatgcg ctgccccgca
      901 tcaagatcga accaccgacc gtttcgatgc agttttcggt caacgactct ccgtttgccg
      961 gcaaagaagg caagtgggtt acctcacgca atattcgcga acgcctcgaa cgcgaactca
     1021 agactaacct cgcactgcgc gtcgaagatc tcggcgactc gttcaaggtt gccggccgcg
     1081 gtgaactgca gctcgctatc ctcatcgaaa atatgcgccg cgaaggtttt gaactcgggg
     1141 tttcaaaacc acaggtaatc ttcaaagaag aaggcgggca aaagctcgag ccgttcgaag
     1201 aaatcgtcat ggatatgccc gagcagtact cgggtggcgt tatccaggag ctcaaccgtc
     1261 gtaagggcat gatgctgatc atggaaacgc tttcgaccgg tttcgtgcgc gtgaagtacg
     1321 aaatgcctac gcgcggcctg atcggtttcc gcagcttttt cctcactgaa acacgtggcg
     1381 aaggttcgat gtcgggcctc tttctcgagt accgtcctta cgcgggcgat ttctcaggcc
     1441 gcacacgtgg cgccattgtt tcgatggaaa gcggtgctac caacgcgtac ggcctcttca
     1501 acatccagga ccgcggcgaa ctttttgtgg gtgcgcagac cccggtatac gtgggcatga
     1561 tcatcggcct gcacgcaaag gataacgacc tggacgtgaa cccgattcgc gagaaaaaac
     1621 tcaccaacgt gcgtgcatcg ggcacagacg aagcactccg ccttgtacct ccccgcaagc
     1681 tttcgcttga acagtgcctt gactttctcg aagacgacga agctcttgag gtaaccccca
     1741 cgagcctcag gcttcgcaag aaggtcctga atccgtcgat gagaaagcgc agcgcctgat
     1801 agcgcatgtt tgagtatttc tctccgcaga atctcaacgc tctgtggcaa cgcctgacca
     1861 gcggtcatgc gttcgcctcg acggccatgg tcgtcggggt gcttctgctg gtacggctga
     1921 tcgtcctgcg catagcttac cgcaatatac aggataaatt cctgcgtctg cggtaccggc
     1981 gcaccgtcag ctatatcata tcgtttattg cgatattcct gttactgccc atctggcttc
     2041 cctcaatccg cagtatcgcg acctttctag gtatattcgg cgcgggcttc ctgattgtga
     2101 cacgcgacct ctgggtttgc gtggtgggct gggcctatat catgatccgc aggcccttta
     2161 tcattggcga tcgcatacag atcggcaata tcgccggtga cgtagttgat atccgcctca
     2221 tggaaacgag cgtcatggag gtatcgcagg cagaaggtgg acagagcact ggccgcgtgg
     2281 tttttttccc gaacgcaaag atatttaccg agagttttgc cactaccggc gccaacgtcg
     2341 ggcatatctt ccaggagctg caggtacatc tttcgaaccg cgccgactgg aaaaccgcag
     2401 cgaacctgct ggaaaaaatt gcccaccgcc gttacgaaga agctctcgcc gccaaagagc
     2461 agctgcagat caacagcgaa gtggatatga tctcttcata ccgtgaaccg cgagtgctcg
     2521 cacagatgtc aggcaaccac attgtgctga agctgcagta catggcgcct accggccagg
     2581 gcgtcaatat gaacgaccac atctggcgcg actttctctc cgcagctcag aaaaatccgc
     2641 gtatccgttt ttcagaatag ccggtaacac agcctgccag gtcgtgttac caggagcctg
     2701 ttagtcttt
//
LOCUS       DAOQJF010000410         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26470_length_2704_cov_4.042658, whole genome shotgun sequence.
ACCESSION   DAOQJF010000410 DAOQJF010000000
VERSION     DAOQJF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26470_length_2704_cov_4.042658"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..705
                     /gene="rdgB"
                     /locus_tag="PLF85_15810"
     CDS             73..705
                     /gene="rdgB"
                     /locus_tag="PLF85_15810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNE21188.1"
                     /translation="MKVLLATENPGKVHEIRGIFHQKLPQTELFCIADLPQDIREKYE
                     AHETGLTYAENSLIKARALAKLADGIIISEDSGFEVEALGRAPGIYSARYAPTDRERC
                     EKILTAIRDVPAPQRRAQFVACVTLMENGKNPLYFFGRRTGTVAAELRGEKGFGYDPI
                     FIPDGETQTWGEAPEKSKILVSHRGLAMGLAIEYLIVRLSQNGERAGRSS"
     gene            717..789
                     /locus_tag="PLF85_15815"
     tRNA            717..789
                     /locus_tag="PLF85_15815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Arg,seq:cct)
     gene            803..1906
                     /gene="prfA"
                     /locus_tag="PLF85_15820"
     CDS             803..1906
                     /gene="prfA"
                     /locus_tag="PLF85_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNE21189.1"
                     /translation="MAELNEQNLKAIVSSFRAIEERLADPQVTADNKRLTELSRERAR
                     LLKTAELAEQWLQLKNQIADCDAGWQAEKDAEMKAELGTELKALREQFAQLSEELQLE
                     LIPKDADSGKNVYLEIRAGTGGDEAGLFARDLFRMYCRYLDAEGIKYEIQEFNETGVG
                     GAKEAILLALGERAYDILHLEAGTHRVQRIPATEANGRIHTSAVTVAVIPEADEDEIS
                     INPADLRIDVFRSSGAGGQHVNKTESAVRITHIPTGIVVGCQEEKSQIKNRAKAMKAL
                     MSKLVEKQRSEAAEKYATEKKAQVGSGDRSEKIRTYNYPQNRVTDHRINFTVHNLDQV
                     MEGRLGDLIGALLLAEREAKMRAGENAVAGVAG"
     gene            1908..>2704
                     /locus_tag="PLF85_15825"
     CDS             1908..>2704
                     /locus_tag="PLF85_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNE21190.1"
                     /translation="MRAMIPPRHSGQVNPTDLFLFTAIILLVAFGLNILLASSSFMAR
                     EQFSDPYFFLKKQAFAAFVGFLGMLAMTQIDPEKYRHLVWPAYVITLVLLVLVFVPGI
                     GKRAGGANRWIVVGGLSIQPSDFAKVSLVLVLARIYSQAKEVDFRLSVTTLAIIALPA
                     FLIGIEQDLGTAIHLVLVAGALLFFTRYPLSMQFGALVAVIAAVSYVIIKTPFRLTRI
                     KAFLDPWESRFDAGFQLVASMKAFLAGGLTGKGFGEELVRHHLQERHT"
BASE COUNT          620 a          788 c          777 g          519 t
ORIGIN      
        1 gaaagcggtc gacaaggcgc tgcgcgacag ctcggtttac agcatgaaaa ttcaaccgcc
       61 aggggacagt ctatgaaagt attgctcgcg accgaaaacc ccggcaaggt gcacgagata
      121 cgcggcatct ttcaccagaa attgccgcag acagaattat tctgcatcgc cgacctgccg
      181 caggacattc gcgaaaaata cgaagcgcac gaaacgggtc tcacgtatgc cgaaaactcc
      241 ctcatcaagg cgcgcgcgct ggcgaagttg gcggatggca tcataatttc agaggattcc
      301 ggttttgaag ttgaagccct gggccgcgca ccgggcatct actccgcgcg gtatgcaccc
      361 accgacagag agcgctgcga gaagattctg acagcgatac gcgacgtacc agccccgcag
      421 cggcgcgcgc agtttgtcgc ctgcgtcacc ctgatggaga acggtaaaaa tcccctgtat
      481 tttttcggcc gtcgcacggg tactgtggca gcagaactac ggggcgaaaa gggtttcggc
      541 tacgacccga tcttcatacc cgatggcgag acgcagacct ggggcgaagc gcccgaaaag
      601 tcaaagatcc tggtgagcca ccgcggcctt gcgatggggc ttgccattga atatctgatt
      661 gtgcgcctgt ctcaaaatgg cgaacgagca ggtcgaagct cttaactaaa gaagcagctc
      721 ctatagctca gatggataga gcagcgccct cctaaggcga agaccccagt tcgactccgg
      781 gtgggagcaa agcgaaacta acgtggcaga actaaacgaa cagaacctga aagcgattgt
      841 ctcgagtttt cgggcaattg aagaacgcct cgccgaccct caggtcacgg ctgacaacaa
      901 gcgcctgacg gagctttcgc gtgaacgcgc ccgtttgctc aagacggccg aactcgccga
      961 gcagtggtta caactaaaga accagattgc cgattgcgac gcgggctggc aggctgaaaa
     1021 agacgccgag atgaaagcag aactggggac tgaactcaag gccttacgtg agcagtttgc
     1081 ccagctctct gaagagctgc agcttgaact gattcccaaa gacgcagatt ccggcaagaa
     1141 cgtctacctc gagattcgcg cgggtaccgg cggcgacgaa gcaggcctgt tcgcgcgcga
     1201 ccttttccgt atgtactgcc gctacctcga cgccgaggga atcaagtacg aaatccagga
     1261 attcaacgaa accggcgtcg gtggcgccaa agaagcgatt ctgctcgcgc tcggcgaacg
     1321 ggcctatgac atcctgcacc tcgaggcggg cacgcaccgc gtgcagcgta tacctgcaac
     1381 cgaggccaat ggccgtatcc acacgtcagc ggttaccgtg gcagtcatac ctgaagccga
     1441 cgaagacgag atcagtatta atcccgccga cctgcgcatc gacgtcttcc gttcatcggg
     1501 cgccggtggc cagcacgtca acaagaccga gtcagccgtg cgcatcacac acataccgac
     1561 gggtattgtc gtcggttgcc aggaagagaa gtcacagatc aagaaccgtg ccaaggcgat
     1621 gaaggcgctg atgtcaaagc tggtcgagaa gcagcgcagt gaggcggctg aaaaatatgc
     1681 caccgagaag aaagcgcagg tgggttcggg cgaccgcagt gaaaagatcc gtacgtacaa
     1741 ctacccgcag aaccgcgtga ccgaccaccg catcaacttc accgtccaca acctcgacca
     1801 ggtcatggaa ggcaggctgg gcgacctgat cggcgcgctc ttactcgcag aacgcgaagc
     1861 taaaatgcgg gccggtgaaa acgccgtggc gggtgtcgcg ggttaacatg cgcgcgatga
     1921 taccgccacg ccatagcggg caggtaaatc ccacagatct gtttcttttt acggcgatca
     1981 ttttgctcgt cgcctttggt ctgaacattc tgctcgcctc ttcgtcgttc atggcgcgcg
     2041 aacagttcag cgacccgtat ttctttctga agaagcaggc ctttgccgct ttcgtcggct
     2101 tcctgggtat gctggcgatg acgcagatcg acccggaaaa ataccggcac cttgtgtggc
     2161 ctgcatacgt gataacactg gtgctgcttg tgctggtctt tgttcccgga atcggtaaac
     2221 gtgccggcgg cgccaaccgc tggatcgtcg tgggtggcct ttccatacaa ccttcagact
     2281 ttgccaaggt ctcgctggtg ctcgtgctgg cgcgtatcta ctcgcaggcg aaggaggtag
     2341 acttccgcct gagcgtcacg accctcgcca tcatcgcgct gccagcattc ctgatcggta
     2401 tagagcagga tctcggtact gcgatacacc tggtactcgt cgcaggcgcc ctgctcttct
     2461 tcacgcgtta cccgctgagt atgcagttcg gtgcgctcgt ggccgttatc gcggctgtca
     2521 gttacgtgat catcaaaact ccctttcgcc tgacgcgcat caaggcattt ctcgacccat
     2581 gggaatcgcg ctttgacgcc ggcttccagc tcgtcgcctc gatgaaagca tttctggccg
     2641 gtgggcttac cggcaaaggt tttggcgaag agctggtgcg ccaccacctg caggaacgcc
     2701 acac
//
LOCUS       DAOQJF010000411         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26613_length_2697_cov_5.487509, whole genome shotgun sequence.
ACCESSION   DAOQJF010000411 DAOQJF010000000
VERSION     DAOQJF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26613_length_2697_cov_5.487509"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..890
                     /locus_tag="PLF85_15830"
     CDS             294..890
                     /locus_tag="PLF85_15830"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNE21191.1"
                     /translation="MYTPAAFALKDIDLIKQLVRDYGFATLVAQRGDDFEATHVPLLW
                     RDSVLIGHLARANDFFTEGQKVLAVFQGPHAYISPAWYEELPAVPTWNYIAIHVHGRL
                     FAESDSAAAAQLEAMVKQYEGKSSAYDYLALREDYRSKMQKAIRAFRIEIERVEAKAK
                     LSQNHSPERVRRVIAQLEKSADTTEQQLAQYMRRWALP"
     gene            complement(887..2149)
                     /locus_tag="PLF85_15835"
     CDS             complement(887..2149)
                     /locus_tag="PLF85_15835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE21192.1"
                     /translation="MPRSQLSPFQLVFDHNPAALAVTRRRDSVIIEVNDAFCALVSYK
                     REEIIGATALNLGIWPQPSDRSRLVAEIESSGRVQNMQVPLRARDHSLRYVEVSSELT
                     DFGGEPCLLTMIIDRTERHHVEQERDQALAEAEQFRAALDNVPAFVYMKDLEHRYTYA
                     NAMTLELFGKTKDTIAGSEDREFFPPEAVERLHEVDRRVFRGERTSEEIEVADTGSGR
                     RIYWEVKAPLYEAGKVKGLVGISTDITQHKNLERRLAHMAYTDYLTGLPNRSYFFEMA
                     QKEFARARRYGEPLAIAMIDLDHFKLVNDTYGHEVGDMVLKEVARVCRVTLRESDLIG
                     RIGGEEFAVLWPNADRAHASDAAERLRAAIDIAKLPLEHGLPVQFTASLGVATITEAD
                     ANIDVILHRADKALYEAKAAGRNCVKLA"
     gene            2285..>2697
                     /locus_tag="PLF85_15840"
     CDS             2285..>2697
                     /locus_tag="PLF85_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE21193.1"
                     /translation="MNLAISDRLKPMLAQIEEFVTKELIPLEPQFFSKPFEELVPLLN
                     EKRNQVKALGLWGPQIPTELGGVGLTLVEHGYVSEALGRSPLGHYVFGCQAPDAGNIE
                     ILHMFGTKEQQEEWLEPLAQGKIRSCFAMTEVDTAG"
BASE COUNT          557 a          761 c          779 g          600 t
ORIGIN      
        1 cgcgcctaag atcttgtacg ggcgaatatc ggcatccagt ttcttcttta atgtggcctg
       61 catgtcgatt tccgtaagga ctccgaaacc ttctttctgc agttcttcgc ggacccgagc
      121 ttcagcggcg gccatgtcgg tcgaaactgt tttgctgatt gcgtaactca tactggcata
      181 ataagcaaga aacagcccgc tggcaaataa tactatacta tgtatagtat agtaagagag
      241 gttctgtttc ccatttgaac tgacggcctg tttcggaggc gaatcagagc agcatgtaca
      301 cacccgcggc ttttgctctc aaggacatcg atcttatcaa acagttggtg cgcgattacg
      361 gttttgccac cctggtggcc cagcggggcg atgactttga ggcgacgcat gttccgctgt
      421 tgtggcgcga cagtgtactg attggccacc tcgcgcgggc aaacgacttc ttcaccgagg
      481 ggcagaaggt gctcgcggtt ttccaggggc cacacgctta catctcgccg gcatggtatg
      541 aagagctgcc cgcggttccg acctggaact acattgcgat ccatgttcac ggtcggctgt
      601 ttgccgagag cgactcggcg gcggccgcgc agctcgaggc gatggtgaag cagtacgagg
      661 gaaaatcctc agcttacgat tacctcgcgc tgcgcgaaga ctatcgcagc aaaatgcaga
      721 aagccattcg cgcgtttcgt attgagatcg agcgcgtcga ggcaaaggcg aagctgtcgc
      781 agaaccactc gcccgagcgc gtccggcgcg ttattgcgca gctggaaaag agtgctgaca
      841 caaccgagca gcagctcgcg caatacatgc gccggtgggc cctcccttag gcgagcttca
      901 cacaattgcg gccggctgct tttgcctcgt agagcgcctt gtccgcccgg tgcaaaatca
      961 catcgatatt cgcatccgcc tccgtgatgg ttgccacacc cagcgatgcg gtgaattgca
     1021 ccggtagacc atgctccagc ggtagcttgg cgatgtcgat ggcggcacgc agtcgttcag
     1081 cggcatcgga agcgtgggcc cggtcggcgt taggccagag cacggcgaat tcttcgcccc
     1141 caatgcggcc tattaggtct gattcgcgca gcgtcacgcg gcagactctt gctacctctt
     1201 tcagcaccat gtctccgacc tcgtgaccat aggtgtcgtt gacgagtttg aaatggtcga
     1261 ggtcgatcat cgcaatcgcc agcggttcac cgtaacgccg tgcgcgggca aattctttct
     1321 gcgccatttc aaaaaaatag ctgcggttcg gcaggccggt gaggtagtcg gtgtaggcca
     1381 tgtgagcgag gcgccgttca agattcttgt gctgcgtgat gtcggtcgaa atgccaacga
     1441 gtcccttgac ctttccggct tcgtacaagg gcgctttgac ttcccagtag atacgccggc
     1501 ccgagccggt atcggcaact tcgatctctt cgctcgtgcg ttcgccccgg aaaacccgcc
     1561 ggtctacttc gtgcaacctt tcgaccgcct cgggcggaaa aaactcgcgg tcttcgctgc
     1621 cggcgatcgt gtctttcgtt ttgccgaaca gttcgagcgt catcgcgttg gcatatgtgt
     1681 agcggtgctc aagatctttc atatacacga atgccggcac attgtcgagc gctgcacgaa
     1741 actgttctgc ctcggcgagc gcctggtcgc gttcttgttc tacgtggtgc cgctcggtgc
     1801 ggtcgattat catcgttagc aggcacggtt cgccgccaaa atcggtaagt tctgacgaga
     1861 cctcgacata gcgcagcgaa tggtctctcg cgcgtagcgg cacctgcata ttctgcacgc
     1921 ggcccgaaga ttcaatctcg gcaaccaacc tgctgcgatc ggatggctgc ggccagatac
     1981 cgaggttgag tgccgtcgcg ccaatgatct cttcgcgttt ataacttaca agagcacaaa
     2041 atgcatcgtt gacttcaatg ataaccgagt cgcggcggcg tgttaccgcc aatgcggcgg
     2101 gattatgatc gaagacgagc tgaaacgggc tgagctgtga gcgcggcata ttggaagggg
     2161 gaaataactg gtacgcttat acagacaggt cgtaaacggt gaatcactct cgtttgccac
     2221 agacttgcga cctgggtatc gcttcgcctt gacccctcga ctacgctcgg ggcaagcccc
     2281 taccatgaat ctcgccatct ctgaccggct gaagccaatg ctcgcgcaaa tcgaggagtt
     2341 cgtaacgaaa gagctcattc cgcttgagcc gcagttcttc tcgaagccgt tcgaggagct
     2401 cgtgccgctg ctcaatgaaa agcgtaacca ggtcaaggca ctcggtctct gggggccaca
     2461 aatacccacc gagctcggtg gtgtgggact aaccctcgtt gagcacggat acgtatcaga
     2521 agcgctcggc agaagcccgc ttgggcatta tgtctttggc tgccaggcgc ccgacgcggg
     2581 caatatcgaa attctgcaca tgttcggtac gaaggagcag caggaagaat ggctcgagcc
     2641 cttggcacag gggaagatcc gcagctgttt tgcgatgacc gaagtcgaca cggccgg
//
LOCUS       DAOQJF010000412         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26619_length_2697_cov_3.603331, whole genome shotgun sequence.
ACCESSION   DAOQJF010000412 DAOQJF010000000
VERSION     DAOQJF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26619_length_2697_cov_3.603331"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2697)
                     /locus_tag="PLF85_15845"
     CDS             complement(<1..>2697)
                     /locus_tag="PLF85_15845"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNE21194.1"
                     /translation="KHFAFTDLYSIDATRGLYESAIYTDRPVYRMGDAVQVRGVFVRK
                     QAAAAHGKIKYRIKNNDNEVIHSGEGELTPAGSVAFTYKTGSLKPARYTIQMEIEGET
                     HSGSFLIEQYKKPETRANMAAVKTILLSGDVVEATVSAAYYSGEALANVPAEVTIERS
                     RISYPWWYGFGFDEYYGDSYDYASWEFVKDYTATLDAAGKLKIAVPTDAKAEADYHYR
                     VRATVKAQNREETSAAVRFKVYRAPVSLRLSQDRWYFSSKTPISFRVSATQVLEQKPA
                     QTSVTAELFRRDYDSKTQKWNDTQVASLELKTNDLGEALGEFAAVARGGVYLVRVKSS
                     AAGRVTHEYLETYVYSAGDFYGDYGEQTERPIAVSPGKKKYELYETAEFAVRMPVQKK
                     LPVLVTLENDRIRKYRLLPAAEAQFLHSEELKSFLSPNFELTVTALDFDKYPRYYSGA
                     AQLVLPPEHRMMKVEVVPDRERYRPGEEANLLVKTFDHKGRPVSAEFSLGLVDEAIYA
                     IREDSLTSLSLALNPRLPYSVVTGNSLQFSFYGYGAEKSLYALYREQMAEAAAMRKGD
                     RNEVRVRKDFRDTAFFTAHGKTGTDGIGRLAVTLPDNLTEWRITAHAHSSAGLAGSER
                     NKLTVSKDFALRLAQPRFLRERDEARLRLLVSNQLKEPQTAVFETTFSSLKLAQPFPA
                     KLTIPAQSERYVDFTVAAPLYPDSGQATLKFIARSEKENDGLQMQLPVLPYGVENYTA
                     TQKIFGDDERAWTTTLKLDADARTDLAELQLSFTPGVIPSVLETLPYLISYPYGCVEQ
                     TLSTFLPAIWAGQSAKKLKLPLPVKQAQLQEITEQGLKKLYGYQHSDGGWGWWSEDPT
                     DLYMSAYTLSGLHEAKLAGIAVDENVLKRGSKYLGEQLATAS"
BASE COUNT          538 a          781 c          737 g          641 t
ORIGIN      
        1 tactcgctgt cgccagttgc tcaccgaggt atttactgcc gcgcttcagc acgttttcat
       61 ccactgcgat tccggcaagt ttggcttcat gcagtcccga gagcgtgtac gcagacatat
      121 aaagatctgt cgggtcttca ctccaccagc cccagccacc atcgctgtgc tggtaaccat
      181 aaagtttctt caggccctgc tcggtgattt cctgcagctg tgcctgtttc accggcaatg
      241 ggagcttcag tttttttgcc gactgccccg cccagatcgc cggcaggaac gtcgacaacg
      301 tctgttcaac gcaaccgtaa gggtaactaa tcaggtaggg gagcgtttcg agtaccgaag
      361 gtattacccc gggggtaaaa ctcagctgca gttctgccag gtcggtgcgc gcgtcggcgt
      421 ccagtttcag cgtcgtagtc caggcgcgct catcgtcgcc gaaaattttc tgcgttgccg
      481 tgtagttctc taccccgtaa ggcaaaaccg gcagctgcat ctgcaggcca tcgttttctt
      541 tttcgctacg cgcaatgaac ttcagtgttg cctgcccgct atccggatac agcggcgctg
      601 cgacagtaaa gtcaacgtaa cgctcgctct gcgcgggaat cgtcagcttt gcggggaaag
      661 gctgtgcaag tttcagtgag ctgaaggtag tctcgaaaac tgcggtctgc ggttctttga
      721 gctggttcga taccagcagc cttagccggg cctcgtcgcg ttcgcgcaag aaacgcggct
      781 gcgcgagccg taatgcaaaa tctttcgaga cagtgagctt gttacgctct gaaccggcca
      841 aacctgcact cgagtgcgca tgcgccgtga tgcgccactc ggtcaggtta tccggcagcg
      901 ttaccgccag acggccaata ccatctgtgc cggtcttacc gtgcgcggta aagaaagccg
      961 tatcgcggaa atccttgcgc acacggactt cattcctgtc ccctttgcgc atcgccgcag
     1021 cctcagccat ctgctcgcgg taaagcgcat acaggctctt ttctgcgcca taaccgtaga
     1081 agctgaactg cagcgaattg cctgtgacga ctgaatacgg cagacgcggg ttgagggcga
     1141 gcgatagcga agtcaaagaa tcttcgcgta tggcgtagat cgcctcatcg acgagcccca
     1201 gcgaaaactc cgcactgaca ggcctgccct tatggtcaaa ggttttcaca agcaggttgg
     1261 cctcttcgcc tggccgatag cgttcacggt ctgggactac ttcaaccttc atcatgcggt
     1321 gctcaggtgg cagcaccagc tgcgccgcac ccgaataata gcggggatac ttgtcaaagt
     1381 cgagcgcagt gactgttaac tcgaaattcg gactcaagaa tgatttgagt tcttcgctgt
     1441 gcaaaaactg cgcttcggca gccggcagta agcggtactt gcggatccgg tcgttttcga
     1501 gggtgaccag cacggggagt tttttctgca ccggcatacg caccgcaaat tcggcagtct
     1561 catagagctc gtatttcttt ttccccgggc ttactgcaat cgggcgctca gtctgttcgc
     1621 cataatcgcc gtagaaatca cccgcactgt agacataagt ctcgagatat tcgtgagtca
     1681 cccggcctgc tgcgctactt ttcacgcgca ccaggtaaac tcccccgcgc gcaaccgccg
     1741 caaattcgcc gagagcttca cccaggtcat tggtcttgag ttcgagcgat gccacctgcg
     1801 tatcgttcca tttctgtgtc ttgctgtcat agtcgcggcg gaaaagttct gccgtgacac
     1861 tggtctgtgc gggtttttgt tcgagcacct gcgtcgcgct gacgcggaag ctgatcggcg
     1921 tcttgctgct gaagtaccag cggtcctggc tcaggcgcag gcttaccggt gcgcggtaga
     1981 ccttgaagcg gactgcggct gaggtttctt cccggttttg cgctttgacg gtcgccctga
     2041 cgcggtagtg gtaatctgcc tcggccttgg catcggtcgg tacggcaatc ttcagtttgc
     2101 cggctgcatc gagtgtcgcc gtatagtcct tcacgaactc ccagctggcg tagtcatagc
     2161 tgtcgccata gtattcatca aaaccaaaac cataccacca gggatagctg atacgcgaac
     2221 gctcgatagt aacctctgcc ggcacattgg ccagcgcttc acccgagtag tacgcggcag
     2281 aaacggtcgc ttctacaaca tcgccgctga gcagaatcgt tttgacagca gccatattcg
     2341 cgcgcgtctc tggcttcttg tactgttcga tcaaaaagct accgctgtga gtctcgcctt
     2401 caatctccat ctggatggta tagcgcgctg gtttgagcga acctgtcttg tacgtgaacg
     2461 caacgctgcc tgcaggtgta agctccccct cgcccgaatg gatcacttcg ttgtcgttgt
     2521 tctttatgcg gtacttgatc ttgccatgcg cagcggctgc ctgtttgcgc acaaaaactc
     2581 cacgcacctg cacggcgtcg cccatgcggt aaacagggcg gtcggtgtag atcgcagact
     2641 cgtaaagacc ccgggtggca tcgatggaat agagatcggt aaaggcgaaa tgtttgc
//
LOCUS       DAOQJF010000413         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26719_length_2691_cov_5.329666, whole genome shotgun sequence.
ACCESSION   DAOQJF010000413 DAOQJF010000000
VERSION     DAOQJF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26719_length_2691_cov_5.329666"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLF85_15850"
     CDS             complement(<1..1221)
                     /locus_tag="PLF85_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21195.1"
                     /translation="MASTGCAGSYAKKIRASEESFYNGDIDTAVANITPLAQDATARD
                     RLLYYMEAGVIYHSKGDYKTSNEIFSRADEMAEEIKTSITGSAKSFLLSDREADFQGE
                     NFERILIKYYLALNHVLLGDLKAAKRSLRKLEADLKEMKYEDAGYKQVLIARYLDALV
                     SEELGQYNDARVQYKNLENFGVDGNWLNHERYLLAQHARDTADMARYSAGREAPLIAP
                     NKGQQTGELVVLLQAGKASVKASRGNLMGDREFALALRIAIDVAILTDGKGLSTAGVI
                     AMLGTAENPIPKFSERERGIAPALSINGVGLPSPAQLTDFDLIAQKNFNDNYSSYINK
                     NVASIATKIVVAAVAADAVARQIRKNNDGIVGALGGLAVGLGAGGAVAATVKPDLRSW
                     HMLPAGYFAVRVILP"
     gene            complement(1257..1841)
                     /locus_tag="PLF85_15855"
     CDS             complement(1257..1841)
                     /locus_tag="PLF85_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNE21196.1"
                     /translation="MRNAMRFLLLAGLFALPACSSTTKRLDAGEDMAADSGELTRGEL
                     EKAAQSLAQKVKLHFDSHRPADGIFVAFLPTKNETSEQLPVNVFDNRIVGDLLGAKIY
                     TVRVEDRSKALSEIAFSQTGATANGISAGKMRSPNFFIQAEITENIFKSGGDRIIEQI
                     LNFELRSVETQLVVFSDRIVYRKKPRNQKGGVTW"
     gene            1945..2592
                     /locus_tag="PLF85_15860"
     CDS             1945..2592
                     /locus_tag="PLF85_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE21197.1"
                     /translation="MEFIIAALDVLLHLERYLDVWAVNYGVWMYAILFAIIFAETGLV
                     VTPFLPGDSLLFAVGAIAARGVLSLPVIILLLAVAAVIGDTLNYSVGRYFGDHVLKRF
                     SRIVKPEYVQKTHDFYEKHGSKMIVLARFVPIIRTLAPFVAGIATMRYREFAFYNVIG
                     GIFWISSMTLAGFAFGNLEFVRRNFSVVVLGIVILSVMPMIIEYVRIRRQKGGEA"
BASE COUNT          567 a          752 c          707 g          665 t
ORIGIN      
        1 cggcagaatt accctcaccg cgaaataccc ggccggcagc atatgccatg acctgagatc
       61 aggtttaacc gtagccgcta cggcgccacc ggcaccgagc cccaccgcaa gaccgccgag
      121 cgcgccgaca atgccgtcat tgttcttgcg aatctgccgc gcgacggcgt ctgctgcaac
      181 tgcggcaacg acgatcttgg tcgcgatcga tgcgacattc ttattgatat aagaggaata
      241 gttatcgttg aaatttttct gcgcaatcag gtcaaaatct gtcagctgcg cgggtgaagg
      301 aagccccaca ccgttgatac tgagcgcagg ggctattcct ctctcacgct cagaaaactt
      361 tgggattgga ttttctgccg tgccgagcat ggcgatgaca ccggctgtag aaagtccctt
      421 gccatcggtc agaatcgcca catcaatcgc gatgcgcagc gccagcgcaa actcgcgatc
      481 acccatgaga ttaccccggc ttgccttgac cgatgcttta cccgcctgca gcagcaccac
      541 gagttcacct gtctgctggc ccttgttagg ggcaatgagc ggggcttcac gacccgcact
      601 gtagcgcgcc atatcggctg tgtcacgcgc atgctgtgcc agcaggtaac gctcgtgatt
      661 cagccagtta ccgtcgacac caaagttttc gaggttcttg tattgtacgc gtgcatcgtt
      721 gtactggcca agctcttcag agaccagcgc atcgaggtaa cgcgcgatca atacctgctt
      781 atagcccgcg tcctcgtatt tcatttcctt aaggtctgct tcgagcttgc gcaggctgcg
      841 cttggccgct ttcaggtcgc cgagcagaac gtggttcagc gccaggtagt acttgatcag
      901 gatacgttcg aaattttcac cctggaaatc ggcctcgcgg tcgctaagca aaaagctttt
      961 agccgaacca gtaatgctgg tcttgatttc ctcggccatc tcatcggcgc gcgaaaaaat
     1021 ctcgttactt gttttgtagt cgcccttgct gtggtaaata acgcctgcct ccatgtaata
     1081 gagcagacgg tcgcgcgccg tggcatcctg tgccaggggc gtaatgtttg ccacggcggt
     1141 atcaatgtcg ccgttataga aagactcttc agatgcgcgg atcttcttcg cgtaagatcc
     1201 cgcgcagccg gttgaagcca ttaagaggag cgcaagtgcg ctcctcttaa tggcgcttac
     1261 cacgtcactc cccctttctg gttacggggt tttttacggt acacgatgcg gtcgctaaaa
     1321 acgaccagct gcgtctcgac ggagcgcaat tcgaagttca gtatctgctc aatgatgcgg
     1381 tcgccgccgc ttttgaaaat attctcggta atttctgcct gtatgaaaaa attggggctg
     1441 cgcatcttgc cggccgatat gccgtttgcg gtagcacccg tctggctgaa ggcaatttca
     1501 gaaagcgcct tgctgcggtc ttctacgcgc acggtgtaga tctttgcacc gagcagatcg
     1561 ccgacgatgc ggttatcgaa tacgttcacg ggtaactgct cagaagtctc attcttggta
     1621 ggcagaaatg cgacgaagat gccatcggcg ggccgatgcg aatcaaaatg aagcttcact
     1681 ttctgcgcaa ggctctgcgc cgctttttca agctcgcccc gggtcaattc gcccgaatcg
     1741 gccgccatat cttcgcccgc atcgaggcgc ttcgtcgtag aactgcacgc aggcagggca
     1801 aaaagtcctg ccagtaagag gaatcgcatt gcattacgca taacccaaaa taagtaaaag
     1861 cgaacgggcg acaaccaaaa ggcccctcgg cgggggttgc agaaggtaat tgtcacccca
     1921 cggtttctaa caaagttagt aaacatggag ttcatcattg cggcgctcga cgtgctgctc
     1981 cacctcgagc ggtaccttga tgtctgggct gtcaattatg gtgtatggat gtatgctatc
     2041 ctgttcgcca tcatttttgc tgaaacgggc ctggtcgtta cgcccttttt gcccggggac
     2101 tctctgttat tcgcggtcgg cgccatcgcc gcccgcggag ttctgtcttt gccggtaatt
     2161 attctccttc ttgcggttgc tgcagtgatc ggcgatacgc tcaattattc cgtcggtcgc
     2221 tatttcggtg accacgtact caaacgcttc tcgcgcattg tgaaacccga gtacgtgcaa
     2281 aaaacccatg atttttacga aaagcacggt tcaaaaatga tcgttctggc gcgcttcgta
     2341 cccattatca ggaccctggc ccctttcgtt gcgggtattg ccacgatgcg ttaccgtgaa
     2401 tttgccttct ataatgtgat cggcggtatt ttttggattt cctcgatgac gctggcaggt
     2461 tttgcttttg gtaacctcga attcgtccga cgcaatttca gcgtggtcgt actcggcatc
     2521 gtcattctat ctgtcatgcc catgattatc gagtatgtgc gtattcgtcg gcagaaaggc
     2581 ggcgaggcat gaaaaaatcc caacagcgcc tcaaaacgca ccgcgcgctt atggtggcgg
     2641 ccatcaagct gatgagcgac gaccgcagct tttcgtcgat cagcctgcgc g
//
LOCUS       DAOQJF010000414         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26851_length_2683_cov_3.530441, whole genome shotgun sequence.
ACCESSION   DAOQJF010000414 DAOQJF010000000
VERSION     DAOQJF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26851_length_2683_cov_3.530441"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLF85_15865"
     CDS             complement(<1..355)
                     /locus_tag="PLF85_15865"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNE21198.1"
                     /translation="MKVAVLTFPGSNCDHDLVEALRTEYQLEAELLWHTRPFTADHDL
                     YFVPGGFSYGDYLRTGALAAQSRSLTSLREAAAQGRPIVGICNGFQILCEAKLLPGAL
                     IRNVNLQHICEWVGLK"
     gene            complement(361..621)
                     /gene="purS"
                     /locus_tag="PLF85_15870"
     CDS             complement(361..621)
                     /gene="purS"
                     /locus_tag="PLF85_15870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNE21199.1"
                     /translation="MKKFAATATVRFKKSVLEPQGKAIQLSLAEKNLHEFTLVRVGKH
                     IEVELDATDQADAQAKMEKIARDYLYNDVMETCEIHVSQVAG"
     gene            complement(640..1535)
                     /locus_tag="PLF85_15875"
                     /pseudo
     CDS             complement(640..1535)
                     /locus_tag="PLF85_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
     gene            complement(1538..2242)
                     /locus_tag="PLF85_15880"
     CDS             complement(1538..2242)
                     /locus_tag="PLF85_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNE21200.1"
                     /translation="MADEQASKGTHARFSEVTSTKLVADRTAAIYSAQKAAFESSEIR
                     NIVTLAHWPGELRDDQKAFALSPLAKDGVAPFATDRPTLFTRPENLLRVVEFLRSDSA
                     FQLDFLLDLTAVDLLGSPREQDNTENLGKRFQVVYLLRSMLPERRAASLRLCVPVDEN
                     EEVPSLIDLYSGANWPEREVFDLMGIRFSGHPNLSRIVLPDNYRGHPLRKDFPVKGIG
                     EDYLIEDLLYKRRNVD"
     gene            complement(2346..>2683)
                     /locus_tag="PLF85_15885"
     CDS             complement(2346..>2683)
                     /locus_tag="PLF85_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21201.1"
                     /translation="WWFARSWWVGAGVNYAALLGGQLKYYDPNVAPGSNPLTPVATPA
                     YWADVAPGGGLFYAQIGTGLRIALGNAFNVVNFEPEVRVLIPLTTPYNNAYGAVVRLN
                     LGFSYAFGL"
BASE COUNT          597 a          710 c          770 g          606 t
ORIGIN      
        1 ctttgagccc gacccactcg cagatatgct gcaggttgac gttgcgtatc agtgcgccgg
       61 gcagaagttt ggcttcgcaa agaatctgga agccgttgca gatgccgaca atcgggcgtc
      121 cctgtgctgc tgcctcgcgc aggctggtga gcgagcggct ctgcgccgcc agcgccccgg
      181 tgcgcaggta gtcaccgtac gaaaatccgc ccgggacaaa atacaggtca tggtctgcag
      241 tgaaagggcg ggtatgccag agaagctcag cctctaactg gtattcggtg cgcagcgctt
      301 cgaccaggtc gtgatcgcag ttgctgccag gaaatgtcag tacagcgact ttcacgtctg
      361 ttacccggcc acctgcgaaa catgtatttc acaggtttcc atgacatcgt tgtacaggta
      421 gtcgcgcgcg atcttttcca tcttggcctg cgcatcggcc tggtcggtgg cgtcgagctc
      481 gacttcaata tgtttgccga cacgcaccag cgtgaactcg tgcaggttct tttcggccag
      541 cgaaagctgg atcgccttgc cctgtggttc gaggactgat tttttgaatc ttactgttgc
      601 ggtagcggca aacttcttca tactttgagc tccccctgac tatcccgccg tttctgccgg
      661 agagatcgaa aggattatat cctgcatttt ttgatagcgt ttcagagttt tgtcaatcac
      721 ttcagccggc aggcggggcg ccggcggagt tttattccag ccgattgatt cgacgtaatc
      781 gcgaatgacc tgcttgtcca tggtgggtat ttcagtgccc gcggcgagcg cgcgctcgta
      841 ttcgccgatt tccacgaagc gggacgagtc aggagtaaaa atttcgtcga tcagcagcag
      901 ttcgccgtcg agcaggccga attcaaactt ggtgtcgagc aggtagatgc ctttttgctt
      961 taacgcctcg aatgcgaaac taaacagagc aaggctgcgc gccttgagag aatctgcgag
     1021 ctgcggtatg cggcgctgca gttcggcaaa cgagatgttc tcgtcgtggc cctggtcatt
     1081 cttcactgcc ggagtgaaag ccggttctgg cagtatctgt cctttcttca gacctggggg
     1141 cagctgcact gccgccaatg taccctgcgc ctggtattct ttgtagcccg agcccatgag
     1201 aaaggcgcgc acgacacact cgtagtcgat gcgctgcgtg cgcttcacca gggctgaacg
     1261 accggcaaat tcaggacgat tgaacggcgg ggggaattcc tggtaatcgg aagaaatgaa
     1321 gtggtgtagc aggtctttca gcagcgaaaa ccagtgtgcc gaaatctgtt gagtatcttt
     1381 cccttaccgg gaatcgtatc ttcaaagacc acatcgaagg cggaaagcct gtcagaggaa
     1441 acaagtagca tcttctcacc gaggtcataa acatcgcgga ctttgccacg gtatgcgggc
     1501 gacggaaggt aggacggcgc accgatggtg tccatggtca gtcgacgttg cgccttttgt
     1561 aaagaaggtc ttcgattagg taatcttcgc cgataccctt cactgggaaa tctttgcgca
     1621 gcgggtgacc acggtagttg tctggcagta caatgcggct gaggttagga tgcccgctga
     1681 agcgtatacc catgagatca aaaacttcgc gctcgggcca gtttgcaccc gaataaagat
     1741 cgatcaatga gggcacttct tcgttctcat cgacgggtac gcagaggcgt aggctcgctg
     1801 cgcggcgctc aggtaacata gaacggagca ggtacacaac ctgaaaacgt ttgcccaggt
     1861 tctctgtgtt atcttgctcg cggggcgagc cgagcaagtc gactgccgtc aggtcgagca
     1921 agaagtccaa ctggaacgca gaatcagaac gcagaaactc caccacgcgg agcaggtttt
     1981 cgggcctggt aaaaagtgtc ggccggtcgg tggcaaacgg tgcgacgcca tctttcgcga
     2041 gcgggctcag cgcgaaggct ttttggtcgt cacgcaattc gcccggccaa tgcgccagcg
     2101 tcacgatgtt gcggatttca gaagattcga aggctgcttt ctgtgcgctg taaattgccg
     2161 cagtacggtc ggcaacaagc tttgtgctcg ttacttcgct gaaacgtgcg tgcgtgcctt
     2221 tggaagcctg ctcatcagcc atacatccaa gagcccgata agactcatca cgcaaataaa
     2281 aaagcccctt gcggggcttt gttttctttc gctgtgtgag tcttgctcac acagcaaaat
     2341 tgccattaaa ggccgaaagc gtacgagaaa cccaggttga ggcgaacgac cgcaccgtat
     2401 gcattgttat acggagtggt cagtggaatc aacacgcgga cttctggttc aaagtttact
     2461 acgttgaaag cgttacccaa cgcaatgcgg agaccggtac cgatctgggc gtagaacagg
     2521 ccgccgccgg gtgcaacatc agcccagtat gcgggtgtgg cgactggagt cagagggttt
     2581 gagccaggag caacattagg atcatagtac ttgagctgtc cacccaacaa tgccgcataa
     2641 ttgacaccag cacctaccca ccatgagcga gcaaaccacc agt
//
LOCUS       DAOQJF010000415         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_26932_length_2678_cov_3.361037, whole genome shotgun sequence.
ACCESSION   DAOQJF010000415 DAOQJF010000000
VERSION     DAOQJF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26932_length_2678_cov_3.361037"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLF85_15890"
     CDS             <1..184
                     /locus_tag="PLF85_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21202.1"
                     /translation="LEFMARRESEGTHRNYSEVYRDIKSSEPIVTLVHSYADDCLLCL
                     DFSVAHIHNPLWHRKI"
     gene            complement(222..1262)
                     /locus_tag="PLF85_15895"
     CDS             complement(222..1262)
                     /locus_tag="PLF85_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE21203.1"
                     /translation="MVLRTAEIRRLYRYETLHHHGKRFRNIWGAHPDSDATKFDPVSG
                     DIRHRALDVAGWLFRKGLGSQKSGQPAQVRPIARDELMSPGPGAKVFWLGHASCLLQV
                     DGLNIITDPVFADRVSPVRFAGPRRLCGLAAPVTDLPRIDAIVLSHNHYDHAESETLR
                     LLARRDQPPVFVPLRVAPLMKQLGFATVYEFDWWQQVELNGYTITCLPCKHFSGRSAT
                     DRNETLWASFMIEFHRSNKRVYFAGDTAYSPHFAEIAAEFSEIDLTLMPIGAYLPRWF
                     MREVHVSPAEAVQAFADLKAQKMLGIHWGTYDLADENLDQPKIEASSAAKALGIGSDR
                     LFVTDVGSSFQF"
     gene            complement(1313..1723)
                     /gene="ruvX"
                     /locus_tag="PLF85_15900"
     CDS             complement(1313..1723)
                     /gene="ruvX"
                     /locus_tag="PLF85_15900"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNE21204.1"
                     /translation="MADRVLCIDFGLKFIGLAVADFPGITARALETIRSDRQDPLQVI
                     GQVVSDEKVTKFLLGFPFSDAEGEIHRRIRDFGDRLAQHFPAIPVELVDESYSSIEAD
                     ELVQQTGLGRRQKQRSQDSQAAKIVLLRYLNAAP"
     gene            complement(1716..2282)
                     /gene="hpt"
                     /locus_tag="PLF85_15905"
     CDS             complement(1716..2282)
                     /gene="hpt"
                     /locus_tag="PLF85_15905"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNE21205.1"
                     /translation="MYEPIPPLIERAKLEARIAELGRQITADYAGKNYIRVIGALRGC
                     FVFMADLIRAIEMPVKVDFMEISSYGNATTSSGNIKILKDLTDDVMGEHVLVAEDIID
                     SGLTLQSMIDLLKGRGVASIEIATLLIKPKNVAAQRKLIYPVKYRAFEVSDEFVVGVG
                     MDYKGYMRNLPYIAQVTDMQQLKLFEGG"
     gene            complement(2286..>2678)
                     /locus_tag="PLF85_15910"
     CDS             complement(2286..>2678)
                     /locus_tag="PLF85_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE21206.1"
                     /translation="VMKRDVITLDATDLLATAARTLIENKIHGLVVMKDGKPWSVLSA
                     FDLLHKSYVESFSDKMDYLRSTLDSLIDQPILHSLKPNDSLDSAARLFTVYGQRTIPV
                     IEGDKLMGIITITDLIRAYGRLVGESGI"
BASE COUNT          592 a          742 c          777 g          567 t
ORIGIN      
        1 cctcgaattt atggcccgcc gggaatcaga aggaacgcac cgtaactatt cagaggtcta
       61 ccgcgatatt aaatcgagcg aacccattgt gacgctcgtg cactcttacg cggacgattg
      121 cctgctgtgc ctcgattttt cggtcgcgca catccataac ccgctctggc atagaaaaat
      181 ctagccagag ctttccagca tgttcctgaa aaaagaacct gctagaactg gaaactactg
      241 ccgacgtcgg taacaaacag gcggtcactg ccaataccca gcgcttttgc agctgagctt
      301 gcttcgattt tcggctggtc cagattttcg tcagcgagat cgtaggtacc ccaatggatg
      361 ccgagcatct tctgcgcttt caggtccgca aatgcctgaa ccgcttctgc cggtgaaacg
      421 tgcacttcgc gcatgaacca gcgcggcagg taggccccga tcggcatcag ggtcaggtcg
      481 atttcactga actccgcggc gatttcagcg aagtgcggcg agtaagcggt atcaccggca
      541 aagtagacgc gcttgtttga gcggtgaaat tcaatcatga aggaagccca gagcgtttca
      601 ttgcggtcgg tagcagaacg accggagaag tgtttgcaag ggaggcaggt gatcgtgtaa
      661 ccgtttagct ccacctgttg ccaccagtca aattcgtata ctgtagcaaa cccgagttgc
      721 ttcatgaggg gggccacccg cagcggaaca aacaccggcg gctggtcgcg gcgggcaagc
      781 aggcgcagcg tttcactttc cgcgtggtcg tagtggttgt gcgagagcac gatcgcgtca
      841 atacgcggca ggtctgttac cggagcggcg agtccacaca ggcggcgcgg gccggcgaat
      901 cgcacgggcg aaacgcgatc agcaaaaacc gggtcagtga taatgttcag gccatcgacc
      961 tgcaagaggc acgaagcatg gcccagccag aaaacctttg cgccggggcc agggctcatc
     1021 agctcatcgc gtgcaatcgg gcgaacctgt gcaggctgac cactcttctg cgagcctaaa
     1081 cccttgcgga acagccagcc agccacgtcg agcgcgcggt gcctgatatc gccgctcacg
     1141 ggatcaaact tggtcgcgtc ggaatcgggg tgagcccccc agatattgcg aaagcgttta
     1201 ccgtgatggt gcagagtttc gtagcgatag aggcggcgga tttcggctgt gcgcaggacc
     1261 acggcgctca gtcggcgatg cgagagtcgc cgtaaacgaa aagctgcccg tgctaaggcg
     1321 cagcgttgag gtatctcagc agcacaatct ttgccgcctg agaatcctgc gagcgttgtt
     1381 tttgccgccg cccgagcccg gtctgctgca cgagctcgtc ggcttcgatg ctgctgtacg
     1441 attcatcgac cagctcgacc ggtattgcgg gaaaatgctg ggccaggcga tcgccgaaat
     1501 ccctgatgcg ccggtgtatt tcaccttccg catcagagaa gggaaaaccg agcaggaact
     1561 tcgttacctt ttcgtcgcta acaacctgcc cgataacctg caaaggatcc tgccggtcag
     1621 agcgtatagt ttcgagtgca cgcgccgtga tgccgggaaa atcggccacc gccaggccga
     1681 taaatttcag cccaaaatca atgcagagga cgcggtcagc caccttcgaa aagttttagc
     1741 tgctgcatat cggtaacctg tgcaatgtag ggcaggttgc gcatgtaccc cttgtaatcc
     1801 atgccgacgc cgacgacaaa ctcgtcggag acttcaaacg cgcggtactt caccgggtag
     1861 atgagcttgc gctgcgctgc gacattcttg ggcttaatca gcagtgtcgc aatctcgatc
     1921 gatgcaaccc cccggccttt aagcaggtca atcatcgact gcagggttaa gccgctgtcg
     1981 atgatgtctt cggcgaccag cacgtgctcg cccatgacgt catcggtcag gtctttgagg
     2041 atcttgatgt tacccgaaga cgtggtggca ttgccgtagc tggaaatctc catgaagtcg
     2101 actttcaccg gcatttcaat cgcacggatc aaatcggcca tgaacacgaa acagccgcgc
     2161 agcgccccga taacacggat gtagttctta ccggcataat cggcagtaat ctgcctgcca
     2221 agttcggcta tacgggcttc aagctttgcg cgctcgatga gtggtggaat cggttcgtac
     2281 atggttcaga ttcccgattc accgacgagc cggccgtacg cgcgtatcag gtcggtaatc
     2341 gtgataattc ccatcagctt gtcgccttcg atgacgggta tggtgcgctg gccatagacc
     2401 gtgaaaagcc gtgctgccga atcgagcgag tcattgggtt tgagcgaatg cagaatcggc
     2461 tggtcgatca ggctgtcgag ggttgagcgc aggtagtcca tcttgtcgga gaaactctcg
     2521 acgtaacttt tgtgcagcag gtcaaaagcg ctgagtaccg accagggttt gccatctttc
     2581 atgacgacaa gcccgtggat tttgttctcg atcaaagtgc gggccgcggt ggcgagcagg
     2641 tcggtcgcat cgagtgtgat cacatcgcgc ttcatgac
//
LOCUS       DAOQJF010000416         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_27179_length_2663_cov_4.256902, whole genome shotgun sequence.
ACCESSION   DAOQJF010000416 DAOQJF010000000
VERSION     DAOQJF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27179_length_2663_cov_4.256902"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLF85_15915"
     CDS             complement(<1..558)
                     /locus_tag="PLF85_15915"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21207.1"
                     /translation="MELVEYLDNSPSPAWATETAAGLLDRSGFSQAESVASASSRQFY
                     LRPHAGLLLAVRLPEKTPKGFRLIGAHTDSPHLRLKANAAQLREGYALLASEVYGGAL
                     LYTWFDRALALAGEIFYRDGESIKSHLVRSKGAIAIVPSLAIHLQRGVNDDGFAPNKQ
                     VALSALVTHRAADFNWNNYLATLAGI"
     gene            611..1504
                     /gene="prpB"
                     /locus_tag="PLF85_15920"
     CDS             611..1504
                     /gene="prpB"
                     /locus_tag="PLF85_15920"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNE21208.1"
                     /translation="MPWMFEKREYDAPQRMKALLAGKEIIQTPGAHDVLTGLMAKKLG
                     FKALYISGAAFSASRGMPDIGYFTVTELSDYVRGLYQATNLPILVDVDTGFGEVVHIP
                     RMVLEMEGAGAAMVQMEDQKLPKKCGHLEGKELVTGDEMCRKIEAFAKTRNKLMLLAR
                     TDAHAMYGIEEAIKRAKMYVNAGADAIFPEALKTEEEFKQFAAEVKVPLLANMTEFGK
                     TPYFKAKDLEAWGYKLVIYPVTSLRSQMKAVERVFSAIKNDGSQEAEVKNMQTRQELY
                     DLIDYAGYGDFDEGVAKSKFI"
     gene            1578..1661
                     /locus_tag="PLF85_15925"
     tRNA            1578..1661
                     /locus_tag="PLF85_15925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1612..1614,aa:Leu,seq:cag)
     gene            complement(1700..2446)
                     /locus_tag="PLF85_15930"
     CDS             complement(1700..2446)
                     /locus_tag="PLF85_15930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE21209.1"
                     /translation="MKSLVLILTTLLVITLHAEDPREYRARKAFEAGSESEQKQIDAI
                     RECAVSKIQTCVYPLIDHLKKEGKENATLRRESANALGRLRAAEARETLLQLLPKEND
                     LQVKAAIVRALGYMGNKADIKTVAGFLGDNDALLRRTAARSLFELNDKTASAEAAGKA
                     AGEKDDYTRVEMLNAALRHEGGKVEHAIALAKILTSQDRAARLRAAEVLGYYANKETL
                     SDLERAKDVEPDPQVRAALSRAIAATLFNN"
BASE COUNT          568 a          766 c          754 g          575 t
ORIGIN      
        1 aatacccgcg agtgttgcca ggtagttgtt ccagttgaaa tcggcagcgc ggtgcgttac
       61 cagagccgaa agtgccacct gcttgttggg agcaaaaccg tcgtcgttca ccccgcgttg
      121 caggtgtatc gccagcgatg gaacgatggc aatggctccc ttgctgcgca ccaggtggct
      181 tttgatactc tcgccgtcgc ggtaaaaaat ttcacccgca agcgcaagcg cccggtcgaa
      241 ccaggtatag agcagggcgc caccgtacac ttcgctggcc agcagcgcat acccttcgcg
      301 caactgcgct gcattggctt tcaggcgcag gtgcggcgaa tcggtatggg cgccaatgag
      361 ccggaaaccc ttcggcgtct tctcaggtag cctgaccgcg agcagcaagc cagcatgagg
      421 cctcaggtaa aactggcggg agctggcaga agccactgac tcagcctggc taaagcccga
      481 tcggtcgagc agccctgcag ccgtttcagt cgcccacgcg ggtgacggag aattatcgag
      541 gtattcgacc agttccatac gaaagacatt atcttgattt gcgcggcgtc gcaaatcaag
      601 ataatgcctc atgccatgga tgtttgaaaa acgcgaatac gacgccccgc agcgcatgaa
      661 agcgcttttg gcgggcaaag aaattatcca gacccccggc gcgcacgacg tgctgaccgg
      721 cctgatggcc aaaaagctcg gcttcaaggc actgtatatc tcgggcgcgg ctttttctgc
      781 ctcgcgcggc atgcccgaca tcggctactt caccgtcacc gaactctctg actacgtgcg
      841 cggcctctac caggcgacca acctgccgat tctcgtcgac gtcgacaccg gcttcggcga
      901 ggtcgtgcat attccgcgca tggtgctcga aatggaaggc gccggtgcgg cgatggtgca
      961 gatggaagac cagaagctcc ccaaaaaatg cggtcacctc gaaggcaaag aactcgtcac
     1021 cggcgatgaa atgtgccgca agatcgaagc tttcgccaaa acacgcaata aactgatgct
     1081 gcttgcgcgc accgatgcgc atgcgatgta tggcatcgaa gaagcgatca agcgcgcaaa
     1141 gatgtacgtc aacgccggcg ccgacgccat ctttcccgaa gcgctcaaga ccgaagagga
     1201 gtttaagcag ttcgcagcag aggtaaaagt gcctctgctt gcgaacatga ccgaattcgg
     1261 taagaccccg tacttcaagg caaaagatct cgaagcatgg ggctataagc ttgtgatcta
     1321 ccccgtaacc tcgctgcgtt cacagatgaa ggctgtggag cgtgtctttt ctgcgatcaa
     1381 gaacgacggc tcacaggaag ccgaagtcaa gaacatgcag acccggcagg agctctacga
     1441 cctgatcgac tacgccggct acggcgattt cgacgaaggg gtggcgaaga gtaagtttat
     1501 ctgagtgcct gacccgcact caaataaact ccaactgaat tgacggcctg tcgtaataac
     1561 ataatttgtt catccgcgcc cagatggcgg aattggtata cgcgctagtt tcaggtacta
     1621 gtgagggcaa cctcgtgggg gttcaagtcc ccctttgggc acaaaattta aactaatgag
     1681 gctgccttgc gcagcctttt tagttattaa acagcgtagc ggcaatcgcc cggctgagcg
     1741 ccgcacgaac ctgcgggtct ggctccacgt ctttggcgcg ctcgagatca gacaaggtct
     1801 ctttattggc gtagtagccg agcacttcgg cggcgcgcag gcgcgcggct cggtcctgac
     1861 tcgtgagtat cttggccagg gcaatggcgt gttcaacttt gccgccctcg tgacgcagcg
     1921 cggcatttag catttctacc cgggtatagt cgtctttttc acccgcggcc ttgccggccg
     1981 cttcagctga agcggttttg tcgttgagtt caaacaggga gcgtgctgcc gtacggcgca
     2041 ggagtgcatc attgtcgcca agaaaaccgg cgactgtctt gatatctgcc ttgttaccca
     2101 tgtatccgag ggcccgcact atggcagctt tgacctgcaa atcgttctct tttggcagta
     2161 gctgcaacag ggtttcccgc gcttcagctg cgcgcaggcg gcccaacgca ttggcgcttt
     2221 cgcgccgcag cgtcgcattc tctttgccct ctttcttcag gtgatcgatc agcgggtaaa
     2281 cgcaggtctg gatcttgctc accgcgcatt cgcgtatcgc gtcgatctgc ttctgctctg
     2341 attcgctgcc ggcttcaaaa gccttgcgcg cccggtactc gcgcgggtct tcggcgtgaa
     2401 gggtaattac cagcagggta gttaggatca gtacaagaga tttcatgctc gcaaaaggtc
     2461 catgggccct gcggtgtcaa ttcccaattc atcaagccgg tagggtagac tgctgcgcct
     2521 tcacacaaat gtcttgccgc cgggcgcggc ggtacttttt cgggggagcg catgggtaaa
     2581 cccggcatct tactgacctt ggtgctctgc cccctgttct tctgccattg tgccgaaaat
     2641 cgtctgacca cctacatgct ggt
//
LOCUS       DAOQJF010000417         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_27391_length_2652_cov_5.549865, whole genome shotgun sequence.
ACCESSION   DAOQJF010000417 DAOQJF010000000
VERSION     DAOQJF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27391_length_2652_cov_5.549865"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLF85_15935"
     CDS             <1..381
                     /locus_tag="PLF85_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21210.1"
                     /translation="THSEERIIAELLLTGEVREAGFRSRVMAPDFLEQQLVSTAAQFI
                     DYLQHELFPQEMLLCQRRSEKLQPLQGRALRAHFDQSLETPTLTLQARIRNQAEFEDT
                     LRRLQNFSFADWLLHCEEERADAD"
     gene            371..1378
                     /locus_tag="PLF85_15940"
     CDS             371..1378
                     /locus_tag="PLF85_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21211.1"
                     /translation="MRIDRLYVERAAGEWPATNEIIKKLEPLETISIEDYQDLFSRRK
                     TNYLEKRDTRNVFIALKRGAMVKEAPPAYGYGASDLHYYYVHAYNCVYECEYCYLQGY
                     FSSPDLVFFVNHGDALEEMQQIAAAHRGKKRVWFHAGEFSDSLALSHISGELPYYWKF
                     FQDNPDTWLELRTKSVNLSVLRSLPPLPNVVVSFSLATEPQAAAYDRETPSVEQRLAA
                     MQRLRDMGFRLGIHFDPIIYAEDFADKMQVIAGQIRKVVGYEAIEYISVGAVRFAKDV
                     YREARSNYADSQMFARHFIQGADTKMRYIRPLRLYLIETAQAMLKKHGCPEEKIYICM
                     E"
     gene            1375..1977
                     /locus_tag="PLF85_15945"
     CDS             1375..1977
                     /locus_tag="PLF85_15945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNE21212.1"
                     /translation="MSERYIVGVTGASGAEYALRFLWQLSFVPGQSDLIVSPNFYKVL
                     ESEAQAQSAGADLLQLATATYGPLGKDHEFVTNDYRDISARAASGSAAYRAMAILPCS
                     MKTLAAIHAGLSQNLIERAADVSLKERRTLILCPRETPLSTVHLRNMLGLTEAGATVM
                     PIMPGYYHRPSGLVDLYDFMTDRIFQHMGITKRTMKPWRS"
     gene            1974..>2652
                     /locus_tag="PLF85_15950"
     CDS             1974..>2652
                     /locus_tag="PLF85_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21213.1"
                     /translation="MSKPGLLFALALATAGLGSQPVESEMDKPLDLSKRWSFYSDETV
                     YLQHQCSASRARAIAVIAQKFFIRVEGHLERRELITPLQLRIYAGADQYRRTLRFSRH
                     REAHYNPRLVMVTAHCGISATVLEEQLALFWLAETGLRTWQRLLLAEALPRMEQVDRF
                     RVVMDKPAKKPAPLAQVLLSDHLPENHERQALAGFVAKLAATGKLKDFLTGLYRDRDA
                     DGTGLDTL"
BASE COUNT          598 a          744 c          746 g          564 t
ORIGIN      
        1 acgcattctg aagaacgcat catcgctgaa ctactgctaa cgggtgaagt gcgcgaagca
       61 ggttttagaa gccgtgttat ggcgccagat tttctggagc aacagctggt ttcaacggca
      121 gcacagttta tcgattatct gcagcacgaa ttatttccgc aggagatgct gctgtgccag
      181 aggcgctcag agaagttgca gcccctgcag ggtagagccc tacgggcgca ttttgaccag
      241 agccttgaaa ctcccacgct gacactgcag gcccgtatac gcaaccaggc tgagtttgaa
      301 gatacgctcc gccgtttaca gaatttcagt tttgctgact ggctgctgca ctgcgaagaa
      361 gagcgcgccg atgcggattg accgactcta cgttgaacgc gccgccggtg agtggcccgc
      421 gacgaatgaa ataataaaaa aacttgaacc gctggaaacc atcagcattg aagactacca
      481 ggatcttttc tcgcgccgca agaccaacta tcttgaaaaa cgcgacaccc gcaacgtgtt
      541 cattgcccta aaacgcggtg caatggtcaa agaggcgccg ccagcatacg gctacggcgc
      601 atctgacctg cactactatt atgtgcacgc atacaactgc gtgtacgaat gcgaatactg
      661 ctacctgcag ggctatttca gctcgcccga cctggtgttc tttgtgaatc acggcgatgc
      721 gctcgaagag atgcagcaaa ttgcagctgc ccaccgggga aaaaaacgcg tgtggttcca
      781 cgcgggggag ttcagcgatt cactggccct ctcgcacatc agcggcgagc ttccttacta
      841 ctggaaattt ttccaggaca accctgatac ctggctcgag ctgagaacca agtcggtgaa
      901 tctgagcgtg ctacgctcgc tgccgccgct gcccaatgtg gtggtaagtt tttcgctcgc
      961 gaccgaaccg caggccgccg catacgaccg cgagacaccg tcggtcgaac agcggctggc
     1021 tgcgatgcag cggctcaggg acatgggctt tcgcctgggc atccattttg acccgatcat
     1081 ttatgcggaa gatttcgcag ataagatgca ggtaatcgcc gggcaaattc gcaaggtagt
     1141 gggttatgag gcgatcgagt atatctcggt cggcgcggtg cgctttgcca aggatgtcta
     1201 ccgcgaagcg cggtcgaatt atgccgattc tcagatgttc gcgcgccact ttatccaggg
     1261 tgcagatacg aaaatgcgct acatccggcc gctacgcctg tacctgatag aaacggcgca
     1321 ggccatgtta aaaaagcatg gttgtcctga agaaaagatt tatatttgta tggaatgagc
     1381 gaacgctaca tcgtcggcgt cactggggcc tctggcgcag aatatgcact gcggtttttg
     1441 tggcagcttt cgtttgtgcc gggccaaagt gacctgatcg tttcaccaaa tttctacaag
     1501 gtgcttgaaa gcgaggcgca ggcgcaaagt gcaggcgctg acctgctgca actcgcgact
     1561 gcgacgtacg ggccgctggg caaagaccac gaattcgtga caaatgacta ccgcgatatc
     1621 tcggcccggg cagcgagcgg gtctgctgct taccgtgcga tggcgattct gccgtgcagc
     1681 atgaagacgc ttgcggcaat tcatgccggc ctcagccaaa acctgattga acgcgctgca
     1741 gatgtttcac tcaaagaacg ccgcacgtta attctctgcc cgcgcgaaac cccgctgagc
     1801 actgtgcacc tgcgcaatat gctcggtctc accgaagccg gcgcaacagt catgccgatc
     1861 atgccggggt attaccaccg ccccagcggg ctggttgatc tctatgattt catgaccgac
     1921 cgtatattcc agcacatggg catcacgaag cgcaccatga aaccctggcg gtcatgagca
     1981 aaccaggttt gcttttcgcg ctcgctctgg cgactgctgg cctcggttca caacccgttg
     2041 aatcagaaat ggacaaaccg ctcgacctgt cgaagcggtg gagcttctac agtgacgaaa
     2101 ctgtttacct gcaacaccag tgttccgcgt cgcgcgcacg cgccattgcc gtgatcgcgc
     2161 agaagttttt tatccgggtc gaagggcacc tggagagacg cgaactaatt acgcctctgc
     2221 agctgcgcat ctatgcgggg gcagaccagt accgtcgcac gttgcgcttt tcccgccacc
     2281 gcgaagccca ctataatccc cggctggtaa tggttactgc gcactgcgga atttcagcaa
     2341 cagtcctgga ggaacagctg gccctgttct ggttggccga gacaggtttg cgtacctggc
     2401 agcgcttgct actcgccgaa gcactgcccc gtatggagca ggtcgatcgc tttcgcgtcg
     2461 tcatggataa gcctgcaaaa aagcccgcgc cgctggcaca ggtgctcttg tctgaccatt
     2521 tacccgaaaa ccacgaaagg caggcgctgg cggggtttgt ggcgaagttg gcagcgactg
     2581 gcaagttgaa agattttctg acagggttat accgcgaccg cgacgccgac ggtacggggc
     2641 ttgataccct gg
//
LOCUS       DAOQJF010000418         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_27604_length_2640_cov_4.530754, whole genome shotgun sequence.
ACCESSION   DAOQJF010000418 DAOQJF010000000
VERSION     DAOQJF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27604_length_2640_cov_4.530754"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLF85_15955"
     CDS             <1..983
                     /locus_tag="PLF85_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE21214.1"
                     /translation="PARIAVEDAEWLKGRVPEISAVAGNVNGRAQVTYSNKNWNTMVQ
                     GAGTDYATIRSATPFMGRMYTKAEEQSRARVALLGLTVVRELYGDNNPIGTFIKINKV
                     SFQVIGVMPAKGGTGFRDQDDVVVVPLTTAMYRLLGKQYVDSLDVEAREGSNIDDVGE
                     SIQRALSTRHRVPASMQEDGYQVMNLADIRAAVTATSSTMSALLASIAAVSLLVGGIG
                     IMNIMLVSVTERTKEIGLRKAVGARRKDILLQFLVETLAVSLSGGALGMLLGAAISLA
                     MSSLAGWSTSVSGFSVVLSVVFSVVVGLVFGIYPARKAAALNPIEALRHE"
     gene            1024..1221
                     /locus_tag="PLF85_15960"
     CDS             1024..1221
                     /locus_tag="PLF85_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21215.1"
                     /translation="MERAVVISRAKLGEDNSLAEHYRSMNADERMQDFLKIQQTYLVA
                     MGYTEFPPVERVIQFRKMNCH"
     gene            1221..1661
                     /locus_tag="PLF85_15965"
     CDS             1221..1661
                     /locus_tag="PLF85_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21216.1"
                     /translation="MPDMRDFLALLQQHSVDFVIIGAFALARFGIPRGTGDLDIFINP
                     TTENAQKVMEVLHDFGLSSLQLTSQDILSGDIIQLGFPPARIDLLTQLTGVTTQEILQ
                     SRTPGKLGPFSVDFLGKSVFLKNKAAVGRAKDLADIEAIRSLEK"
     gene            complement(2028..>2640)
                     /gene="tpiA"
                     /locus_tag="PLF85_15970"
     CDS             complement(2028..>2640)
                     /gene="tpiA"
                     /locus_tag="PLF85_15970"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNE21217.1"
                     /translation="TAAKAALAGSKVKIGIQNCSAEKDGAFTGEISASQARDAGAQYV
                     LIGHSERRHVLKESDEFLIKKIERVLEQNLVAMLCVGETLAERESGRAEAVVLEQLGA
                     ALSNLTAEQWANISIAYEPVWAIGTGKNATPEDAQSMHETIRKRVAELNKDIAPKIRI
                     LYGGSVKPDNIAALMAKPDIDGALVGGASLKADSFGDLIKFAR"
BASE COUNT          568 a          755 c          745 g          572 t
ORIGIN      
        1 gccctgcgcg cattgccgtc gaagacgccg aatggctcaa ggggcgcgtg ccggaaatca
       61 gcgcagttgc gggcaacgtc aacggccgcg cacaggtaac ctacagcaac aagaactgga
      121 acaccatggt acagggagcc ggcaccgact acgcgacgat ccgcagcgcg acgccgttca
      181 tgggccgcat gtacacaaaa gccgaagagc agtcccgcgc acgcgtagcg ctgctcggac
      241 tgaccgtcgt gcgcgaactc tacggcgaca ataacccgat cggtacattc atcaagatca
      301 acaaggtaag cttccaggtg atcggcgtca tgccggccaa gggcggcacc ggttttcgcg
      361 accaggatga cgtggtggtg gtgccgctga ccacggccat gtaccgcctg ctcggcaagc
      421 agtacgtcga ttcgctcgac gtagaagcac gcgagggcag caacatcgac gacgtaggag
      481 aatccataca gcgagcgctg tcgacccgcc atcgcgtgcc ggcatcgatg caggaagacg
      541 gctaccaggt catgaacctc gccgacataa gagcagcagt gacggcaact tcttcgacaa
      601 tgtcggcgct gctcgcatcg attgcggcag tctcactgct tgtcggcggc atcggtatca
      661 tgaacatcat gctcgtctcg gtcaccgagc gcacgaaaga aattggtttg cgcaaagccg
      721 tcggcgcacg acgtaaagac attttgctgc agtttttggt agagacgctc gcggtcagcc
      781 tgtcaggcgg agctttgggt atgctgctgg gtgcagcgat ttcgctggcc atgtcgagcc
      841 ttgccggctg gtcgacgtca gtgagcggat tttcggtcgt gctctcggtg gtattctcgg
      901 tagtagtcgg cctcgtgttc ggtatctacc ccgcgcgcaa agcggctgca ctcaacccga
      961 ttgaagcgtt gcggcacgag tgaaatttga tttacagtcc gctgtctcct aatatgggtt
     1021 ggtatggagc gtgcggtcgt catcagcagg gcaaagctgg gcgaagacaa ttcgctggca
     1081 gaacactacc ggagtatgaa tgctgatgag agaatgcagg actttcttaa gatccagcag
     1141 acctacctgg tagcgatggg ctacaccgaa tttccaccgg tcgagagggt aattcaattt
     1201 cggaaaatga actgtcactg atgcctgata tgcgcgattt tctcgcgctc ttgcagcagc
     1261 actcggtcga ctttgtgatc atcggggcat ttgctctcgc ccgatttggc attccgcgcg
     1321 gcacaggcga cctcgatatc ttcatcaacc cgaccaccga aaacgctcag aaggtcatgg
     1381 aagtcttgca tgactttggc ctgtcatctc tgcagttaac ttcgcaagat atcctctcgg
     1441 gtgacattat ccaattggga tttccccccg cacgcatcga tttgctcacg cagcttaccg
     1501 gtgtcactac ccaggaaata ctacagtcga ggacaccggg aaagctggga cccttttcag
     1561 tcgattttct tggcaagtct gtcttcttga agaacaaagc agccgtaggc cgcgcaaaag
     1621 atctcgcaga tatcgaagcg atccgatcac tggagaaata gccatgcggg gttttatcgt
     1681 accactttgc ctggttgcgg cctgcatcgc accgcgcaac cagacgctca ctgatgaaac
     1741 ctatgcgcaa cgatattcca ccggatcggc aagccgtggt agtaactcgt tcaccagtga
     1801 tagcggtggt tcggcgctta taccgacagc gattatggca gcgcccgctc tatgccgggc
     1861 catcgaccag tcccgcagtc aaagcgagcg aacggcccgg tgtaatgagc tttttcggca
     1921 cctgagcaac agcgacgccg cagcgcacga atggcagagc tattacatac gctgtcagaa
     1981 aatctgaggt agctcttttt gctatgggct taagcccata gcaaaagtta tcgggcgaac
     2041 tttatcaaat cgccaaaact atctgccttc aggctcgcgc cacccaccag cgcaccgtcg
     2101 atgtcgggtt tcgccatgag cgcggcgatg ttgtcgggct tgaccgaacc gccgtagaga
     2161 atacgaattt tgggagctat gtctttattg agttctgcga cgcgcttgcg gatggtctcg
     2221 tgcatgctct gcgcatcttc aggcgtcgca ttcttacccg taccgatcgc ccagacgggt
     2281 tcataggcga tggaaatatt cgcccactgt tctgcggtca ggttcgagag cgcggcgccc
     2341 agttgttcga gcacgacagc ttcggcgcgg cctgactcgc gctccgccag ggtttcaccg
     2401 acacagagca tcgcgaccag gttctgttcg agcacgcgct caatcttttt tatgagaaat
     2461 tcatccgatt ctttcaggac atgccgccgc tcggagtgcc cgatcagaac gtactgggct
     2521 cccgcatcgc gcgcttgtga cgcggatatt tcacccgtga acgcgccgtc tttttcggcc
     2581 gaacaattct ggataccgat cttcactttt gaacccgcga gcgcggcctt ggcggccgtg
//
LOCUS       DAOQJF010000419         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_27820_length_2628_cov_4.699184, whole genome shotgun sequence.
ACCESSION   DAOQJF010000419 DAOQJF010000000
VERSION     DAOQJF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27820_length_2628_cov_4.699184"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLF85_15975"
     CDS             <1..635
                     /locus_tag="PLF85_15975"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNE21218.1"
                     /translation="IFGPAKKDQLFFLSGPSFAREMALKMPTAITIAGYATDRLPEIQ
                     KVFHNEYFRAYATPDVTGVELGGALKNVMAIATGIADGLGLGSNTRAAIITRGLAEIA
                     RLGEKLGAKPMTFMGLAGMGDLVLTCTGDLSRNRTVGLKLGQGMSLAQIQGEMRMVAE
                     GVPTTESAYELSQQLGVDLPITTAVYRILYESKPAPEAIKELMARSIKFE"
     gene            684..>1122
                     /gene="msrB"
                     /locus_tag="PLF85_15980"
     CDS             684..>1122
                     /gene="msrB"
                     /locus_tag="PLF85_15980"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNE21219.1"
                     /translation="MKRYVLAIALFLIIAGAADTLAENKSARGGLPRLQRVQKSDAEW
                     KKLLTPAQYYVLRQQGTERAFTGKFHNHHEKGSYVCAGCRLPLFSSATKFDSGTGWPS
                     FFAPVKAAHVEELEDRSHGMLRTEVRCGRCGGHLGHVFDDGPRG"
     assembly_gap    1123..1222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1303..2574)
                     /locus_tag="PLF85_15985"
     CDS             complement(1303..2574)
                     /locus_tag="PLF85_15985"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNE21220.1"
                     /translation="MPMFITAFGSGLIASFSACVYPLIPITTAIFAGGERKSWAQGFR
                     AAGIYTLGMSLTYGTLGMIAALSGSVFGRYLGDNRAILIIGLVLIYLGLAYAHLVPLP
                     LPNLANRLQVQKKDNLLFPLVLGIFSAFIAAPCTAPFFGSLLVQISKNAAENQSILQP
                     AFSAFAFALGMGVPFLLIGVFSIRLPKPGIWLKAVEYLGAVVLITAGFHYIEDISGDF
                     PPAAQAFPFAVIGLAMFATFFVLAEPLKAEYHMGKRMKLNTAGMLLISALGLFLFTSP
                     FVKYWGVEVKTNTGATSDAGRKSAMALPQGRLKWYQDLQEAKALAAKEKKILLIDFWA
                     DWCTACEEMEKKLFVKDEFIDFALANNILPVRVDYSTPTDALDRLAKDYNIRGLPTVV
                     LTRADGELIHTLTGFYSKDYTMRELKSALKD"
BASE COUNT          588 a          672 c          728 g          540 t
ORIGIN      
        1 aaatattcgg gccggcaaag aaagaccaac tattcttttt gagtggacca tcttttgccc
       61 gcgagatggc tctcaagatg cccacggcaa tcaccatcgc gggttatgcc accgaccgtt
      121 tgcccgaaat acagaaggtt ttccataacg aatacttccg ggcatacgca acgcctgacg
      181 taacaggtgt cgagctcggt ggggcactca agaacgtgat ggcgattgcc accggcattg
      241 cagacggcct tggtcttggc agcaatacgc gcgcggccat tattacgcgt ggccttgcgg
      301 aaatagcacg cctcggcgaa aaactcgggg ccaaaccgat gacgttcatg ggcctggctg
      361 gcatgggtga ccttgtgctg acctgcacgg gcgatctgtc gcgcaaccgt acggtcggtc
      421 ttaagctggg ccagggtatg tcgctggcgc agatccaggg cgagatgcgc atggtggccg
      481 aaggtgtgcc gacaaccgaa agtgcgtatg aattgtcgca acagctgggc gtagacctgc
      541 ccatcacaac tgccgtatac cgcattcttt acgagagtaa gcccgctccc gaggccatca
      601 aagaactcat ggcgcgctcc atcaagtttg aataaaattc ctgtgcagta attatcttac
      661 tttgttattt taagtggcag gatatgaagc gatacgttct ggcgattgcg ctgtttttga
      721 taatagccgg tgcagcggat acactggctg aaaataaatc cgccaggggt ggtctgcccc
      781 gtttgcagcg cgtgcagaaa agcgacgctg agtggaagaa gctcctgacc cctgcgcagt
      841 attacgtact gaggcagcag ggtaccgagc gcgcattcac gggaaagttc cacaaccacc
      901 atgaaaaggg ttcatacgtc tgcgccggtt gccggttacc cttgttttct tcagcgacaa
      961 agtttgattc aggtaccggc tggccgagtt tttttgctcc ggtaaaggca gcgcatgtgg
     1021 aagaacttga agaccgcagt cacggcatgc tgcgcaccga ggtccgctgc ggccgctgcg
     1081 gcggccattt gggtcatgtc tttgacgatg ggccacgagg gcnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nngacgggcc acgccccacg gggctgcgct actgcatcaa
     1261 ttctgccgct ttgcagttcc tgaaagactg atccccgggg ggtcagtctt tcagggcaga
     1321 cttcagctcg cgcatagtat agtctttgct gtaaaaacct gtcagcgtgt gtatgagttc
     1381 cccatcggcc ctcgttagga caacggtggg caatccgcga atattgtaat cttttgccag
     1441 cctgtcgagt gcgtccgtag gtgttgagta atcgacgcgt accggcagaa tattattcgc
     1501 cagtgcaaag tcgatgaact cgtctttgac gaagagtttc ttttccattt cttcgcaggc
     1561 ggtgcaccag tcggcccaga agtcaatcag cagaatcttc ttctcttttg ctgccagcgc
     1621 tttggcttcc tgcagatcct ggtaccattt caggcgcccc tgtggtaacg ccattgcact
     1681 tttgcgccct gcatcgctgg tggcaccggt attggtcttc acttccacgc cccagtattt
     1741 cacaaacggc gaggtaaaga gaaagaggcc gagcgctgaa atcagcagca taccggctgt
     1801 gttgagtttc attcttttgc ccatatgata ctccgctttg agtggttctg cgagcacgaa
     1861 gaaggtcgcg aacatggcga ggccgataac ggcaaacgga aatgcctgcg cagccggcgg
     1921 gaaatccccg ctgatgtctt cgatatagtg aaagccggca gtgatcagca cgacggcgcc
     1981 caggtattcc acggccttca gccagatgcc gggtttgggc agcctgatag agaaaacgcc
     2041 aatcagcaga aagggaactc ccataccgag agcaaaggca aaggctgaaa atgccggctg
     2101 cagaatggac tggttttcag ccgcattctt gctgatctgc accagcaggc tgccgaaaaa
     2161 cggagcagta caaggtgcgg cgataaaggc ggaaaaaata ccgagcacga ggggaaacag
     2221 caggttgtct ttcttctgca cctgcaggcg attcgccagg ttgggcaatg gcagcggcac
     2281 gaggtgcgca tatgccagcc cgaggtagat gaggacgagc ccaatgatca ggattgcacg
     2341 gttatcgccg aggtaacggc cgaaaacgct gcccgagagc gctgcaatca ttccgagtgt
     2401 gccgtaagtc agcgacatgc cgagcgtata gatgccggct gcgcgaaacc cctgcgccca
     2461 gcttttgcgc tcaccgccgg caaagatcgc ggttgtaatc ggtatcaatg ggtaaacgca
     2521 cgcggaaaaa gaagcaatga gacccgaacc gaacgcggtg atgaacatcg gcataactgc
     2581 aatatacgaa aactgcgttc cggcggcgag cagttaccga atgagccc
//
LOCUS       DAOQJF010000420         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_27928_length_2622_cov_4.855863, whole genome shotgun sequence.
ACCESSION   DAOQJF010000420 DAOQJF010000000
VERSION     DAOQJF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27928_length_2622_cov_4.855863"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLF85_15990"
     CDS             <1..267
                     /locus_tag="PLF85_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21221.1"
                     /translation="EFFYDRKEMVRMVIDDLLDASNRYRDKFEISTKATGDMIMAVKL
                     LEGMRRVALAVKDDAMAAKAKTGIEAYKARIEAEKDEKKAPAKK"
     gene            345..800
                     /locus_tag="PLF85_15995"
     CDS             345..800
                     /locus_tag="PLF85_15995"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNE21222.1"
                     /translation="MKRFFPFVLITFFATASLSGNTDPAAGEPTEISWKTLRGFNLKT
                     GKMSDMLTKLNGKAVKIPGFMVPLDDMDYESVGRFLLVPDPQACIHVPPPPANQMIFV
                     VMKGKKKAPVAFGMPIMLTGDLKVKSIRSQFGVVSFQVAGESVKPYTGK"
     gene            797..1489
                     /locus_tag="PLF85_16000"
     CDS             797..1489
                     /locus_tag="PLF85_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE21223.1"
                     /translation="MKRAVIEAENLAFAYAANSILEISRLRVNAGSHTLICGDSGCGK
                     STLMNLISGVLSGSTGNLRVLGQDLSALSPAKRDRFRAQHIAVIFQQFNLIPYLTVTE
                     NILLALRVAGQKKDHARIAAILDHLQIAHVARQRASELSHGQQQRAAAARALALNAEL
                     ILADEPTSALDDTNAKLFLELLFGEAETNSRTIVVVSHDLRYRKRFDQVIDLAEVNRA
                     VQKSRPRARKSA"
     gene            1486..>2622
                     /locus_tag="PLF85_16005"
     CDS             1486..>2622
                     /locus_tag="PLF85_16005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE21224.1"
                     /translation="MIRLVFASLINRRVIVFLTALSVALSVFLFASVEKLRDGARASF
                     THTVSGTHIVAGARSGQIPLLLFSLFHLGSPTSNVSYASYMKYRKHPAVAWALPLSIG
                     DSHRGFRVVGTSPFLMKYYRYRNGQQLKLAEGSYSLARFKAVLGSTAARELGYKVGAP
                     LVLTHGISDVQGIHDHEESPFTVEGIFEPTGTPFDKGIYISLESAELMHDEHGAEHAE
                     GKGQHDHQADPVKVEKITSFMIGVKQPVDILPLMRAINEDTAEPLTAILPGVVLSEIW
                     SMMGYAENILKLVSYLVMLTALCGLFIALYASLAERYREMAILRALGAHPGGIASLLV
                     AESLLLALAGIAAGYGLLALAAATLHSTLLRYFSVSLGVFPLTGQ"
BASE COUNT          553 a          747 c          753 g          569 t
ORIGIN      
        1 gagttcttct acgaccgcaa agaaatggtg cgcatggtga ttgatgacct gctcgacgcc
       61 tcgaaccgct accgcgataa attcgagatt tcaactaagg ctaccggcga catgatcatg
      121 gcggtcaagc tgcttgaggg catgcgccgg gttgcgctgg cggtaaaaga cgacgccatg
      181 gcggcaaagg ccaagaccgg tattgaagcc tacaaggccc gtatcgaagc ggagaaagat
      241 gagaaaaaag ctcccgcgaa gaagtagttt taaggccatg aggcccatac ctgaaaacgc
      301 ttgacgtaag cgaaaacaat gccggtgaca cagcagtgtc atccatgaag cgtttcttcc
      361 cctttgtatt aattactttt ttcgccactg catccctcag cggcaacacg gaccctgctg
      421 cgggcgagcc cactgaaatc agctggaaga ccctgcgcgg ctttaacctc aagaccggca
      481 agatgtctga catgctcacg aagctgaatg gcaaggctgt gaagattccc ggtttcatgg
      541 tgccgcttga tgacatggat tacgagtcgg tggggcgctt tctgctcgtg cccgaccctc
      601 aggcctgcat acacgtgccg ccgccaccgg cgaaccagat gattttcgtg gtcatgaaag
      661 gaaaaaagaa agcccccgtg gccttcggca tgcccatcat gctgaccggc gatctgaagg
      721 tgaagtcgat tcgctcgcag tttggcgtgg tttcgttcca ggtggcaggt gaatcggtaa
      781 agccttacac gggaaaatga aacgcgcggt catcgaagca gaaaacctgg cattcgccta
      841 cgcagcgaat agcattcttg aaatctcgcg gttgcgggtg aacgccggca gccatacgct
      901 tatctgtggc gacagcggtt gcggcaaaag cacgctgatg aaccttatct ccggtgtgct
      961 ctcgggctcc accggcaacc ttcgtgtgct ggggcaggat ttgtcagcgc tgtcgccggc
     1021 aaagcgcgac cgcttccgcg cgcagcacat cgcagtgatc tttcagcagt tcaacctgat
     1081 accttacctg acggtgacag agaatatttt gctcgcgttg cgtgtcgccg ggcaaaagaa
     1141 agaccatgcc cgcattgctg caatactcga tcacctgcag attgcgcacg tggcacggca
     1201 gcgggcgagt gaactcagcc atggccagca gcagcgtgct gcggcggccc gggcgctggc
     1261 gctgaatgca gaactcattc ttgccgatga accaacgtcc gcgctcgacg ataccaatgc
     1321 gaagttgttt ctcgagttac tctttggcga ggcagaaaca aacagccgca ccattgtggt
     1381 ggtctcgcac gacctgcgct accgcaaacg cttcgaccag gtgatcgatt tggcagaagt
     1441 gaacagggca gtgcaaaagt cccgcccgcg ggcaaggaaa agcgcatgat ccgcctggtt
     1501 tttgcctcgc tcatcaaccg gcgtgtgatc gtctttctga ccgcactctc cgttgcgctc
     1561 agtgtttttc tttttgcctc ggtcgaaaaa cttcgcgatg gcgcacgcgc cagcttcacg
     1621 cacaccgtca gcggcacaca cattgtcgcg ggcgcgcgtt cgggccagat tcccttgttg
     1681 ctgttttctc tcttccatct cggttcaccc acgagtaatg tctcctacgc aagctacatg
     1741 aagtaccgca aacacccggc tgtggcctgg gcgctgccgc tctcgatcgg cgacagccac
     1801 aggggttttc gcgtcgtggg taccagcccc tttttgatga aatactaccg ctaccgcaac
     1861 gggcagcagc tcaagctggc cgagggcagc tattcgctcg ctcgcttcaa ggccgtgctg
     1921 ggcagcacgg ccgcgcgcga acttggctat aaagtgggag ccccccttgt tctgacgcat
     1981 ggcatcagcg atgtgcaggg catccatgat catgaagaaa gcccgtttac ggttgagggt
     2041 atttttgaac ctaccgggac gccgttcgat aagggcatct atatttcgct cgaaagcgca
     2101 gagctgatgc acgatgagca tggcgcagaa cacgccgaag gcaaagggca gcatgaccac
     2161 caggctgacc cggtgaaggt tgaaaagatc acgagcttca tgatcggcgt gaagcagccc
     2221 gtcgatatac tgccgctgat gcgcgcgatc aacgaagaca ccgccgagcc cctgacggcg
     2281 attctgccgg gcgttgtgtt gagcgaaatc tggagcatga tggggtatgc cgagaatatc
     2341 ctgaagctcg tttcttacct cgtcatgctg accgcgctct gcgggctttt tatcgctctt
     2401 tatgcctcgc ttgcagagcg ctaccgtgaa atggcgatct tgcgtgcgct gggtgcacat
     2461 cctggcggca ttgcctcgtt actggttgcc gaaagcctct tgctcgcgct ggcgggtatc
     2521 gcggcgggct atggcctgct cgctcttgcg gccgcaactc tgcattcgac attgctgcgc
     2581 tatttttctg tttcgctggg tgttttcccg ctcacggggc ag
//
LOCUS       DAOQJF010000421         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_28114_length_2614_cov_3.973818, whole genome shotgun sequence.
ACCESSION   DAOQJF010000421 DAOQJF010000000
VERSION     DAOQJF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28114_length_2614_cov_3.973818"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLF85_16010"
     CDS             complement(<1..398)
                     /locus_tag="PLF85_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNE21225.1"
                     /translation="MWQKFRRFAYRLCEDTDSGLGRYFNLLMMGLILISISVWLLESH
                     KGISESYGAALKIVEDTAIICFMAEYILRMIAYRGPVWRYMLQPLAVVDILAIVPFFV
                     TAGNDMVILRVLRLFRIFRLIKLVRYSEAV"
     gene            complement(407..>2614)
                     /locus_tag="PLF85_16015"
     CDS             complement(407..>2614)
                     /locus_tag="PLF85_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21226.1"
                     /translation="LRSAMKLGNPTKTEWPAETLPMNSRLFASGFWNYSFFQFNRKFL
                     PPLWAEEQYNPASASFLPRSHNILSMNQTHRNWVGIGFPGRTLEASVDMAGGIMVYPG
                     SYTLEFATLEKGRLVRPQEKSAEVKLLLKSSDRIVCAWRGLVVEYTATEHGISVKATG
                     NKPLLVSLRPFNVEGPALVYKLHYSEKKRHLTGDADLQFDKSPDHALVSDWKNGDALR
                     RIVPQVRRLGSRKKATLPAHDVKDNIGLTTAAFYFESADRCSISVTDHQHDPLPSPLK
                     GKTAREVAEEYFGQTIQAELPKDYSDWLSHARNHLIALWDYDSIKPGSYTYHHFWIRD
                     AVIMMYALLLLGAKKAVKPIIERFTGMVRPSGLFQSQTGEWDANGQALWILAQYVRFT
                     HDTSIIVRMRKQITRMVSWIEKACDQHGGVLPPGFSAEHLGPADWYLWDNLWALGGLK
                     AITPYLKPLGLSGRAEAIFTKLSGALQRYLNHYSYLPAALGRGKDAGMIGSIAAAYPL
                     QLHEFCDNRMLRTLDLIRDNYFFNGCFFQENIHSGLNPYLTLQMAESYLHIGRTRQAR
                     QILRSIRRRAQKAYTFPEAIHVRTGGGCMGDGFHGWASAESIIMIRNLMVREVTFSDG
                     TEGLIWLSGYRDKWLKQTSQAQNLCTPWSVTAFRLENMRLSLRGLSNAVTNIISLPEG
                     YEATNAQNGRPLREIAYERICRSNSAERRYFAIDNASDTAELMLSPKGKVNPD"
BASE COUNT          585 a          698 c          745 g          586 t
ORIGIN      
        1 tgtactgcct ctgaatagcg caccagcttg ataaggcgga agatgcggaa aagccgcagc
       61 acccggagaa taaccatgtc gttacccgcg gtgacgaaaa agggcacgat cgcgagaatg
      121 tcgactaccg caagcggttg cagcatgtat cgccagactg gccctctgta tgcaatcatg
      181 cgaaggatat actcagccat gaagcagatg atagccgtat cttcgacaat tttcagcgca
      241 gcgccgtaac tttccgaaat ccctttgtgc gattcgagca gccagacgct gatcgatatg
      301 aggatcaacc ccatcatcag caggttgaag taacggccaa gacccgagtc ggtgtcttcg
      361 cacaggcggt aagcgaaacg ccggaatttt tgccacatgg aaggtgttag tccgggttaa
      421 ctttcccttt cgggctaagc attagctccg cagtatctga cgcattgtcg atggcaaaat
      481 agcgacgctc agccgaattg ctgcggcaga tgcgctcata ggcaatttcg cgcagggggc
      541 ggccgttctg tgcattcgtt gcttcgtaac cctctggcag cgaaatgata ttcgtcaccg
      601 cgttcgaaag tcccctgagg ctcaggcgca tattttcgag ccggaaagcg gttaccgacc
      661 acggagtgca gaggttctgt gcctggctcg tctgcttcag ccatttgtca cggtaacctg
      721 aaagccagat cagcccttca gtaccatcgc tgaaagtgac ttcccgtacc atcaggttac
      781 gtatcattat gatggattca gccgaggccc agccgtggaa cccgtcaccc atacagccgc
      841 cgccggtgcg cacgtgaata gcttcgggaa acgtataggc tttctgcgcg cgccgccgga
      901 tgctgcgcag aatctggcga gcctgccgcg tgcggccgat atgcaggtaa ctttctgcca
      961 tctgcaacgt caggtaggga ttcaggcccg agtggatatt ctcctggaaa aagcagccat
     1021 tgaagaaata gttgtcacga atcaggtcga gtgtgcgcag catgcggttg tcgcagaatt
     1081 cgtgtagttg cagcgggtag gcggcggcaa tcgagcctat cataccggcg tctttaccac
     1141 ggccaagcgc tgctggcagg taggaataat gattcaggta acgttgcagt gcgcctgaaa
     1201 gcttggtgaa aattgcctca gcgcggccgc tcagtccgag cggtttcagg tagggagtta
     1261 tggccttcaa cccaccgagc gcccacaggt tgtcccacag gtaccagtcg gccggaccga
     1321 ggtgctcggc ggaaaatccg ggtggcagca cgccgccgtg ctggtcgcag gccttctcaa
     1381 tccagctaac catccgcgta atctgtttgc gcatccgcac aataattgaa gtgtcatgcg
     1441 tgaagcgcac atactgggca agaatccaga gtgcctggcc gttggcatcc cactcgccgg
     1501 tctgcgactg gaaaagtccc gaaggtctca ccatgcccgt gaagcgctca atgatgggtt
     1561 tgacggcctt cttggcgccg agcaacagca atgcatacat catgatgact gcatcgcgga
     1621 tccagaagtg gtgataggtg tagctacccg gtttgatcga atcatagtcc cacagggcga
     1681 tgaggtgatt acgggcgtgg cttagccagt cgctgtaatc tttcgggagt tctgcctgta
     1741 ttgtctggcc aaaatattct tcggcaactt cacgcgcagt tttacccttg agcggcgagg
     1801 gcaatgggtc atgctggtgg tcggtgacag agatggagca gcggtcggca gattcaaagt
     1861 agaatgctgc cgtagtcagg ccgatgttgt cttttacatc atgcgcgggt aacgtcgctt
     1921 tttttctgct gccgagccgc cgcacctgcg gcacaatacg ccgcagcgcg tcgccgtttt
     1981 tccagtcaga aaccagtgca tggtcaggtg atttgtcaaa ttgcaggtcc gcatcacccg
     2041 tcaggtggcg tttcttttcg ctatagtgaa gcttgtacac gagggcaggc ccctcaacgt
     2101 tgaaaggacg caatgatacc aacaatggtt tgttacctgt agccttcacg gatataccgt
     2161 gttcggtggc tgtgtattct actaccagac cacgccaggc gcatacaatg cggtcagatg
     2221 acttgagcag cagcttgact tctgcagatt tctcctgtgg gcggacgagg cggcctttct
     2281 ccagtgttgc gaattccagc gtgtaagaac ccggatacac cataatacca cccgccatgt
     2341 cgactgacgc ttcgagtgtg cgaccaggaa agccaatacc cacccagttc cggtgtgtct
     2401 ggttcatcga gaggatattg tgcgagcgtg gaagaaaaga tgccgaggcc gggttatatt
     2461 gctcttcggc ccacaacgga ggcagaaact tgcggttaaa ctggaaaaag ctgtagttcc
     2521 agaagcccga cgcaaacaac cgggagttca tgggcagagt ttctgccggc cactctgttt
     2581 ttgtcggatt gccgagcttc atcgccgagc gcag
//
LOCUS       DAOQJF010000422         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_28229_length_2608_cov_6.390913, whole genome shotgun sequence.
ACCESSION   DAOQJF010000422 DAOQJF010000000
VERSION     DAOQJF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28229_length_2608_cov_6.390913"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..804
                     /locus_tag="PLF85_16020"
     CDS             139..804
                     /locus_tag="PLF85_16020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE21227.1"
                     /translation="MSSPENSVRIVLADDHPIILQGLRRLLEGDARYNVIAECENGQK
                     LVDLLKQREADLVVCDLSMPKLNGLDAIREIKKLRPLIKILILSMHKDPQIFEKALAL
                     GVDGFVLKEDIYDQLLFAVQTVNAGGKSYSPRLTEALATGKADRGDLAAEILTRRERE
                     ILRHAALGKTNRDIADELDISVRTVESHRANLMHKLDLENVQELVAFALKQGVVSVDE
                     IES"
     gene            981..1271
                     /locus_tag="PLF85_16025"
     CDS             981..1271
                     /locus_tag="PLF85_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21228.1"
                     /translation="MKIAKYVMVAALSGISALAAVDVVQGEDKPAGCEKIADIRVGDL
                     FNRHSRDIAYDGVIDEAKKMGAQKVSVQLVTHSHPKLGKSYTATGVAYRCGK"
     gene            complement(1283..2287)
                     /locus_tag="PLF85_16030"
     CDS             complement(1283..2287)
                     /locus_tag="PLF85_16030"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNE21229.1"
                     /translation="MRFGAFIDTYYSSSAYRPASRDRQFITQAARDREFNINLAHIDA
                     AVDHDRVRGRLALQFGTAVNANYQNETTAEKYSNQLSVRNLQEAYAGYKLFDRLWLDM
                     GIFFGHIGFENWISHNNWNYTRALYADNTPYYATGARLAYEVTARLKLQLQLLNGWQV
                     ITPPNRDKSIGTQVSYEFSTVFKIVHNGFAGNVAPDDTTTQYRFYSNLIFEVNPLRAI
                     AFALSADVGAQKNPTNNDYRAWYTGAFYVRWLITEQLRTALRLEYFIDRSEMLVKTGT
                     TNGFSVGGLTTNLDYAPTDMYRLRLEYRNFFSRDAVYAFTDAARSQEHIVTVAASLRI
                     "
BASE COUNT          613 a          680 c          716 g          599 t
ORIGIN      
        1 tacatgacga cggccaggga tttgacactg caggtgccgg tggagggcag gggctcgtta
       61 ccatggccga acgcgcggcg attattccag gagtgctcac ggtgcactca acgcccggca
      121 aagggacgga ggtaaaactt gtcatcccct gaaaacagcg tgcgcattgt gttggctgac
      181 gaccacccga ttattctgca ggggttgcgc cgcctgctcg agggcgacgc acgttacaat
      241 gttatcgcag agtgcgaaaa cgggcagaag ctcgttgatc ttctgaagca gcgcgaggcc
      301 gatcttgtcg tctgtgacct gtcgatgcca aaactcaacg gcctcgatgc cattcgcgaa
      361 ataaagaagc tccgcccatt gatcaaaata ctgattcttt cgatgcacaa agacccgcag
      421 atttttgaaa aggcgctggc ccttggcgtc gatggctttg tgcttaaaga agacatctac
      481 gaccagctac tgttcgccgt gcagacggtg aacgcaggtg gcaaaagtta ttcaccccgc
      541 ctaaccgaag cgctggcaac cggcaaggct gaccgcggtg acctggccgc cgaaattctc
      601 acccgccgcg agcgcgagat actgcgccat gccgccctcg gcaaaaccaa ccgcgacatc
      661 gccgacgaac tcgatatcag cgtgcgcacg gtcgaatcgc accgtgccaa cctgatgcat
      721 aagctcgacc tggaaaacgt gcaggaactc gtggcgttcg cgctcaagca gggtgtggtt
      781 tccgtcgatg agatagaatc gtagccaaat agcccctttc tcggggcaaa ttgcgtacta
      841 atactgagtt tttgcagcgg taacgccgat gccatttttc cggaatatac cgtaattatc
      901 ggtatgagga accagggacg gcaggagagg acatcttgtc cgccgtctct ggaaaacctc
      961 gtgtatcata ggagaataaa atgaaaatag caaagtatgt aatggtagcg gctctttctg
     1021 gtatttctgc actggcggca gtagatgttg ttcagggtga agacaaaccc gctggctgcg
     1081 aaaagattgc cgacatcagg gtcggtgacc tattcaaccg ccattcacgt gacattgcct
     1141 atgacggagt gatcgacgag gctaaaaaaa tgggcgcgca aaaggtctct gtccagctcg
     1201 taacacactc gcacccgaag ttgggtaagt cgtacactgc gaccggtgtc gcgtaccgct
     1261 gcggcaaata agcccggcga cctcaaattc tcaggctcgc ggccacggtc actatgtgct
     1321 cctggctgcg agccgcgtcg gtaaacgcgt aaactgcatc gcggctgaaa aagttgcggt
     1381 actcgaggcg tagccgatac atgtccgtcg gggcatagtc aagattcgtt gtaagtcccc
     1441 caacagaaaa accattggtc gtgccggttt ttaccagcat ctcgctgcgg tcgatgaagt
     1501 actcgaggcg cagtgccgtt ctcagctgct cagtgattag ccagcgcaca taaaatgccc
     1561 cggtgtacca cgccctgtaa tcattgttag tcgggttttt ttgtgcaccc acatctgccg
     1621 acagggcgaa tgcgatagcc cttagcggat tcacttcaaa aatcaggtta ctgtaaaaac
     1681 gatactgggt agtcgtgtca tcaggcgcta cattgcccgc aaagccattg tgtacaattt
     1741 tgaataccgt tgagaattca taagaaacct gtgtgccgat gctcttgtcg cgattgggcg
     1801 gcgtaatcac ctgccacccg tttaaaagct gcaactgcag cttcagcctg gccgttactt
     1861 catacgccag tcttgcccct gttgcgtaat agggtgtatt gtccgcgtag agcgcacgcg
     1921 tatagttcca gttattgtgg gagatccagt tttcgaagcc aatatggccg aaaaaaatac
     1981 ccatgtccag ccagagacgg tcgaatagtt tgtaacccgc atatgcctcc tgcaggttgc
     2041 gcaccgataa ctggttagag tatttttctg cagtcgtctc attctggtaa ttcgcgttca
     2101 ctgcagtgcc gaattgcagt gcgagcctgc cacgcacccg gtcatgatcg accgcggcat
     2161 cgatgtgcgc taggttgatg ttaaattcgc ggtcacgcgc tgcctgtgtg ataaactgcc
     2221 tgtcgcgtga tgcgggtcga taagcgcttg aagagtagta agtatcgatg aaagccccga
     2281 accgcaccgg cggtgattgc gggacgactg tgttcccgct tggttcctgt gcatatactg
     2341 atgtcccggc cagaagaagc gcagaaagta acatggcatt cattgaaaat ggaggaatca
     2401 atgctctcat caggaattct tcaggctctc ggggtgcttc tgccggttgt gacaggaaat
     2461 ccactacttt gcatttgctg ccaaaattgc gcaatttgtt gacggggtca tttgcggggg
     2521 tagttcgcct tatggaattc ttcatcgata cggccgatgt ggccgaaatc aaggccgcgc
     2581 atgaactggg tattctcgac ggcgtcac
//
LOCUS       DAOQJF010000423         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_28264_length_2606_cov_5.802430, whole genome shotgun sequence.
ACCESSION   DAOQJF010000423 DAOQJF010000000
VERSION     DAOQJF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28264_length_2606_cov_5.802430"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /locus_tag="PLF85_16035"
     CDS             <1..1670
                     /locus_tag="PLF85_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNE21230.1"
                     /translation="FVDGRIHNAEQTKSQWETREKYLHQVEVIYNEPSDDSLRAGLDR
                     FWQSWQELAQFPEEMSHRELVRTRGREVTFRMRDTFQKLSDLRRQADFEFEVTVGRIN
                     DMGTQIRDLNDRIMKAQAVGDRPNDLMDKRDALVQELSKLADITLEKQDPDEFIVYLG
                     GEMLVQGRVQHKILLQGDAANEGMKKAVWEHNGQKALFRAGSAQGLLEMRDITIRENI
                     EKMDLLAINLSDTVNEVHRDGFGLTKETNINFFRIDPLSRNIRGNYDLSGDGVDDVSA
                     IFKIAGKNALTADRPIGVAGTMTFVKNDVEHTPVYVTYRPDMSLQDVIHAINRSDAGV
                     VAYLNHNNNLVLKGKEASDNYLTNFMIRHVEDSGEFLTGFSGLLQNSGTAGAFDYRRI
                     DEINKLQATLDRITLTSTIHPAGAMYLSNEVEGNTSLIAAGLGKDVGGTGDANMAFGA
                     KDGGNATRIAQAMRHKSTMVGEYRNSDEFYNALIAKAGIESKTAKEQVDNQKLILTNL
                     ENLRQSVMGVNLDEEMAAMVQFQHGYNAAARVMQTMNELLDKLINHL"
     gene            1780..>2606
                     /gene="flgL"
                     /locus_tag="PLF85_16040"
     CDS             1780..>2606
                     /gene="flgL"
                     /locus_tag="PLF85_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNE21231.1"
                     /translation="MIRVTNLMQNNTMLSTLNRHQVALEKTERELATGSKIQLPSSDP
                     VGAANQMLYRTRLTELEQYDRNVAEAKDRLNLMDGQIDRVGEIFQRMRYLTVQAANGT
                     NSGFELKEAVAKEINQHLLALIDIANSKDSTGRSLFGGSVIERDPFLPVYSHLQTAGI
                     DQGNAVTGVQYQGDIQVQNREIERNEAMDITLPGNRVFWGTNTSVASNKDSSGYVAQG
                     DQLIAINGTRIQVSAGDTINDIIDKINTAPVEVRASKGSQDDIILTSKSPHQIWLEDL
                     "
BASE COUNT          664 a          720 c          708 g          514 t
ORIGIN      
        1 aatttgtcga tggccgcatc cacaatgccg agcagacaaa atcacagtgg gagacccgcg
       61 aaaaatacct gcaccaggta gaagtcatct acaacgaacc ctctgacgat tcgctgcggg
      121 cgggccttga ccgtttctgg caatcgtggc aggaactcgc gcagtttcct gaggagatga
      181 gccaccgcga gctggtgcgc acgcgtgggc gtgaagttac cttccgcatg cgcgatacct
      241 tccagaaact atctgacctg cgtcgccagg cggatttcga gtttgaagtg accgtgggcc
      301 gtataaacga tatgggtacg cagatccgtg acctcaatga ccgcatcatg aaggcacagg
      361 cagtcggtga caggccaaac gacctgatgg acaaacgcga tgcccttgtg caggaactct
      421 ctaagctcgc tgacattacc ctcgagaaac aggaccccga cgagttcatc gtctacctcg
      481 gcggtgagat gctcgtgcag ggacgtgtgc agcacaagat actgctgcag ggtgatgcag
      541 cgaacgaagg catgaagaag gccgtgtggg agcacaacgg gcagaaagca ctcttccgtg
      601 ccggtagcgc acagggcctg ctcgagatgc gcgatatcac gatccgcgaa aatatcgaaa
      661 agatggattt gctcgcgatc aacctctccg acacagtgaa tgaagttcac cgtgatggtt
      721 ttggtctcac caaagaaacc aacatcaact tcttccgcat cgatccgctc tcgcgcaata
      781 tccgtggcaa ctatgatctt tcaggtgacg gtgtcgacga tgtcagcgcg atttttaaga
      841 tcgcgggtaa gaacgcgctc actgctgacc gcccaatcgg cgttgcgggt acgatgacct
      901 ttgtcaagaa tgatgtggag cacactcccg tatacgtgac ctaccgcccc gatatgtcac
      961 tgcaggatgt cattcatgca atcaaccgtt cggatgcggg tgttgtggca tatctcaacc
     1021 acaacaacaa cctggtcctg aagggcaaag aagcctctga caactacctc acgaatttca
     1081 tgatccgcca tgtggaagac tctggggaat ttcttaccgg cttctcaggc ttgttgcaga
     1141 attcaggtac tgcgggtgcg tttgattacc ggcgcattga cgaaatcaac aaactacagg
     1201 caacgctcga ccgcattaca ctgacctcga caatacaccc cgcaggtgcc atgtatctct
     1261 ctaacgaggt cgaaggcaac acttcgctca ttgcggccgg ccttggcaaa gacgtcggcg
     1321 gtaccggcga tgcgaacatg gctttcggtg cgaaggacgg cggaaacgcc actcgtatcg
     1381 cgcaggcgat gcgccataaa tccaccatgg tgggcgaata ccgcaacagc gacgaattct
     1441 ataacgcgct cattgccaaa gcaggtattg aatccaagac cgccaaggag caggtcgata
     1501 accagaagct gattctcacc aacctcgaga accttcgcca gtcggtcatg ggtgttaacc
     1561 tcgacgaaga gatggcggcg atggtgcagt tccagcatgg ttacaacgca gccgcgcgcg
     1621 tgatgcagac aatgaacgag cttttggaca aattgattaa tcatttatga taaagcgccc
     1681 cacccccagc ccctccccaa gtttagggag gggagtaaga attgccttcc cccagacttg
     1741 ggggaagggt tagggttggg ggcgtaacta ggagtatata tgatacgcgt aacaaacctg
     1801 atgcagaaca acacgatgct ctcgacgctc aaccgccacc aggtggcgct cgaaaagacc
     1861 gagcgtgaac tggcaacggg ctcgaaaata cagctgccgt cgagcgaccc ggtgggcgcg
     1921 gcaaaccaaa tgctctaccg caccaggctg acggagctcg agcagtatga ccgcaatgtt
     1981 gccgaagcca aggatcgtct caatctcatg gatggccaga tcgaccgcgt gggcgaaatt
     2041 ttccagcgga tgcgttacct gaccgtacag gctgccaatg gcaccaatag tggctttgaa
     2101 ctcaaagagg cggtcgccaa ggaaatcaac cagcacctgc tggcattgat tgatatcgcc
     2161 aattccaaag actccaccgg gcgctcgctc tttggtggca gcgtgatcga gcgcgatccc
     2221 tttttgcccg tctattcgca cctgcagaca gccggaatcg accagggcaa cgcagtcacc
     2281 ggcgttcagt accagggtga catacaggta cagaatcgcg agattgaacg caatgaggcg
     2341 atggatatca cgctacccgg taaccgcgtt ttctggggca cgaatacttc agtcgcctca
     2401 aacaaagatt cttcgggtta cgtcgcacag ggagaccagt tgatagcgat caacggtacc
     2461 cgcatccagg tttcggccgg cgacacgatc aacgatatca tcgacaagat caatacagcg
     2521 cctgtcgagg tgcgcgcgag taagggctcc caggacgata ttattctcac aagtaaatct
     2581 ccgcaccaga tctggctcga agacct
//
LOCUS       DAOQJF010000424         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_28317_length_2603_cov_3.893250, whole genome shotgun sequence.
ACCESSION   DAOQJF010000424 DAOQJF010000000
VERSION     DAOQJF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28317_length_2603_cov_3.893250"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLF85_16045"
     CDS             complement(<1..1106)
                     /locus_tag="PLF85_16045"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNE21232.1"
                     /translation="MQENSAPPKMLFLKHVSRIVLFYFIGLSVFFISGFVVFKVLFAD
                     TEKAIVPDVVGRLYLTDHNMLRDDFKVELKSAYLVQYPYGYILAQNLTPGRKVDKNTK
                     LELLVNLSDAIVQVPKLTGFSEELVDGSIASLPVGGRIFSLRKGVITRVPSAQPKREV
                     LAQFPPAGTPVLPNTPVSLLVSDGPAQPGTPQASLAPTLEKGIPVSIALGAAYHLKKP
                     ATITLRNTDKADENATLLSEAKITGDSIALEVGQFVENLASGKSVIDLEDLPFTQLFV
                     PAKKLGDQNQVLTVARREAIPNEDGSPYSEFWLVSNSSPLPVFRRRDDSFDVFRDYYA
                     AESSVKPVEQKTDSSQTKTIVGETQAPVATIPVA"
     gene            1205..2149
                     /locus_tag="PLF85_16050"
     CDS             1205..2149
                     /locus_tag="PLF85_16050"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNE21233.1"
                     /translation="MKKLQPVWYDELPEKQVMPVLRDNILFLFINPTEYHGPHLPLAN
                     DYLISRGIADRLARVLIDEGLTERYVAGPLINLGLDPTPGAGTINTSPQRFAALLATW
                     ADKLPLIGAKRVILMTFHGAPRHNHALQRLVDKLAARGVKALNPLNLLLAHMVRYQPG
                     DFADALQPVKDASLRAELDRSLGRDYHAGFFETSLTLALAPESVAPGRRELPDCPPLT
                     IPAWQKAAIHGMRSLGQGDAAAELLFAAEALAWLNLKPFPGYTGMPRLANEKSGEIFV
                     SRILGEYLQATRAVFVNGEAPPAPVLGVTDLISGLAGI"
     gene            complement(2157..2498)
                     /locus_tag="PLF85_16055"
     CDS             complement(2157..2498)
                     /locus_tag="PLF85_16055"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE21234.1"
                     /translation="MPVSIAPQKLAELRLLGGDALVSQLLAKFLESSSSLIAQAEVAL
                     QSGDAAKVDFCVHTLKGSAMSLGLTEMSDLLVALNTRTKARQLEGMAADFTQLAVLLE
                     AVRQYKAQNFP"
BASE COUNT          507 a          742 c          780 g          574 t
ORIGIN      
        1 tgtgctaccg gtatcgtcgc gacaggtgcc tgggtttcac cgacgatggt cttggtctgg
       61 ctggaatcgg tcttttgttc tacaggctta acgctgcttt ctgctgcgta gtagtcgcgg
      121 aagacatcga agctgtcatc gcggcgccgg aataccggca ggggggagct gttgctgacc
      181 agccagaatt cactgtaggg cgaaccatct tcgttaggga tggcctcgcg ccgtgcgacg
      241 gtcagtacct ggttctggtc accgagtttt ttcgctggaa caaagagctg ggtgaagggt
      301 aggtcttcaa ggtcgatgac cgatttgccg ctcgccaggt tttcgacaaa ttggccgact
      361 tcaagcgcga tgctgtcacc ggtaattttc gcctcgctga gcagcgtggc gttttcgtct
      421 gccttgtcgg tattgcgcag cgtgatagtg gcaggtttct tcaggtggta ggccgcgccg
      481 agcgcaatcg aaaccgggat gcctttttcg agtgtcggtg caaggcttgc ctggggagtt
      541 ccgggctgtg ccgggccgtc agagaccagc agcgataccg gtgtgttcgg caggacgggt
      601 gtgcctgccg gggggaactg cgccaggact tcgcgtttcg gttgtgccga aggaacccgc
      661 gtaatgactc ccttgcgcag cgagaagatg cggccaccca cgggcaggct cgcgatcgaa
      721 ccatcaacga gttcttcaga aaatcctgtc agcttcggca cctgtacaat cgcgtctgaa
      781 aggtttacca gcagctcgag cttggtattc ttgtctacct tgcggccagg cgtcaggttc
      841 tgcgccagga tgtagccgta gggatactgc accaggtagg cagacttcag ttcaaccttg
      901 aaatcgtcgc gcagcatgtt gtggtcggtc aggtacagcc ggccgaccac atcgggcacg
      961 atggcctttt cggtatcggc gaaaagaacc ttaaagacga cgaagccgga aatgaaaaag
     1021 accgataacc cgataaaata gaacagcacg atgcgggaga catgtttcag gaacagcatc
     1081 ttcggggggg cagaattctc ttgcatggct gggttattac ctaccggcaa aaccgggtgt
     1141 aaagagattc gggtgctgtc ttctttgcgt tgaccgggca aatcgggcgt gattttgttc
     1201 gcgcatgaag aagctgcagc ccgtctggta cgatgaattg ccggaaaagc aggtaatgcc
     1261 ggtcctgcgc gacaacatcc ttttcctctt tatcaatcct acagaatacc acggcccgca
     1321 cctgccgctt gcgaatgact acctgatcag ccgcggtatt gctgaccggc ttgcccgggt
     1381 tttaatcgat gaagggctga ccgagcgcta cgtcgccggg ccgctcatca acctgggact
     1441 tgaccctaca cccggtgcgg ggaccatcaa tacctcgccg cagcgtttcg ctgcgctgct
     1501 ggcaacctgg gcagataagc tcccgctgat cggggcaaag cgcgtgattc tcatgacttt
     1561 ccacggcgcg ccgcggcata accatgctct gcagcgttta gtcgataagc tcgcagcccg
     1621 cggcgtaaag gcactgaacc cactcaacct gctgcttgcc cacatggtgc gctaccagcc
     1681 cggcgatttt gctgatgccc tgcagccggt aaaagatgcg tcgcttaggg cggagctcga
     1741 tcgttctctc ggccgcgact accatgccgg ttttttcgaa acttcgctga cactggcgct
     1801 tgcgccggaa agcgtcgccc ccggtcgccg cgagctgccg gattgcccgc cgttaacgat
     1861 acctgcctgg cagaaagcgg cgattcacgg tatgcggtcg ctggggcagg gcgatgccgc
     1921 cgccgaactg ctctttgctg ccgaggcttt ggcctggctg aatctcaagc cgtttcccgg
     1981 ctacacgggc atgccgcgcc tggccaatga aaaaagcgga gagatattcg tctcacgtat
     2041 tctcggcgaa tacctgcagg cgacgcgcgc tgttttcgtc aacggtgaag ctccgccagc
     2101 cccggtactc ggcgtcaccg acctgatttc aggtctcgcg ggaatctaac agaagcttac
     2161 gggaagttct gcgccttgta ctggcgcact gcttcgagca gcacggcgag ctgcgtaaaa
     2221 tctgccgcca tgccttccag ctggcgggct ttggttcggg tattaagtgc gacgagcagg
     2281 tcgctcatct cggtcaggcc caggctcatt gcgctgccct tcagcgtgtg cacacaaaaa
     2341 tcaaccttgg ctgcatcgcc tgactggagt gctacctctg cctgtgcaat cagcgaagag
     2401 gagctttcga gaaattttgc cagcagctgg ctgacaagcg cgtcgccacc gagcagacgc
     2461 aactcggcga gtttttgggg cgcgatgctg acaggcatcc tgcagactgt tttgctgtgc
     2521 tataccgtca acggcgattg ccctgtaata gaagtttacg caccgtccga cacacgaccc
     2581 ctgccatacc gtgcggcgga tca
//
LOCUS       DAOQJF010000425         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_28558_length_2590_cov_3.951874, whole genome shotgun sequence.
ACCESSION   DAOQJF010000425 DAOQJF010000000
VERSION     DAOQJF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28558_length_2590_cov_3.951874"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..657
                     /locus_tag="PLF85_16060"
     CDS             196..657
                     /locus_tag="PLF85_16060"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNE21235.1"
                     /translation="MRLDLTSTNFGASPFSISLWFKLNVVPASINTVWQKGTAMTFWN
                     GLRFNQDNANSFIITYGNGASGSNLSHPSVAAATWYHMAFTYDGSAGTLYMAPHGGSL
                     SKYGPTTGNYAQNAAALQINPGTLNMCVDDLLHYNRALHADEVQQNFESLE"
     gene            complement(676..1890)
                     /locus_tag="PLF85_16065"
     CDS             complement(676..1890)
                     /locus_tag="PLF85_16065"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE21236.1"
                     /translation="MNVYALLTPIAIFFVLLEIAACFVFKRKYINFQEAVANFGTALG
                     NQTTNLLVAAGVYEVYGWLHANYRVAGPLEMTPVNFILLLLGVDFIFYWVHRWGHEMK
                     IMWAAHSPHHSAEEMNFFVALRASVTQRLFSFFFFWPLTLVGFTPFDIYTMTALHLFI
                     SFLHHTEFIPKLWRPIEFIFTTPSHHRVHHGANYQYLDKNFGEFLIIWDRMFSTFAEE
                     REKIVYGMYNHPQSFNPIKINFHYYVVIFREMLKAPGLFNKLRIWFMPLSWSPEGKKP
                     LPPEITVQNQKRYQSVMFDGAKTYLVVHAILTAVLMLFVISPKSPLSYEMKWVGAILL
                     WHSIINWAGILESKRWLLPSELLRLTLTAVFVFQVTRRMEIQAVVCSLAVFSTLWSMN
                     YFRLQRPHTAAA"
     gene            complement(1935..2513)
                     /locus_tag="PLF85_16070"
     CDS             complement(1935..2513)
                     /locus_tag="PLF85_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE21237.1"
                     /translation="MQVDTKQKILEKAGALFYAQGYNNTGIDEITKMCGIKKPALYYH
                     FESKTGLGLAYLEYRAEMLFGMLDHLLGRSSTFDKYLSSWATALFVLAKRGEFFGCPF
                     TAFASELSLDERPLFTPQLNSVQERWLRFQARAYLQYHENSAAAKQVARRILVAHTGC
                     VMLYRASRDLQYLKQLKAEFAAIAAAAEEYNA"
BASE COUNT          618 a          674 c          737 g          561 t
ORIGIN      
        1 aagatgctga caacgagaga agtcgttctg ggaaaattcg gtcggcacat aagtctatgt
       61 caaaatcggc ccaaacagcg cgtcaacgct tttggattca gctaaaatag ccctattgtc
      121 gccggtgaag ttgtgacaag attcctacta tgctaccgcg ccggtgaggc cggtaaggcg
      181 ctttgtgtga gttccgtgcg gcttgatctg acctccacga atttcggggc ttcacctttt
      241 agtatcagcc tgtggtttaa attgaatgta gtaccagcca gtatcaatac ggtttggcaa
      301 aaagggacgg cgatgacgtt ctggaacggc ctgagattca accaggacaa tgcaaacagc
      361 tttatcatca cttatggcaa cggagcctca ggcagtaacc tgtctcaccc ttcagttgct
      421 gcagccacgt ggtaccatat ggcgttcact tacgatggca gcgcagggac tctctatatg
      481 gcgcctcatg gtggatctct cagcaaatat ggccccacaa cgggcaacta tgcccagaat
      541 gctgcggcgc tacagattaa cccgggcacg ctcaacatgt gcgtggacga cctcctgcat
      601 tacaatcggg cattgcacgc tgacgaagtt cagcagaatt ttgaatcact cgaatagccg
      661 cacaggcgca ggcacctaag ctgcagcggt gtgggggcgc tgcagacgaa aatagttcat
      721 gctccataat gttgagaata ccgccaggga gcaaacgact gcctgaattt ccatacgccg
      781 cgttacctga aaaacaaaaa ccgcagtgag agtgagtcgc agaagctctg acggcaatag
      841 ccagcgtttc gattcgagaa taccggccca gttgatgata ctgtgccaca agagtatagc
      901 tcccacccat ttcatttcat aagacagtgg ggatttcggt gagatcacaa acagcatcag
      961 cacggcagtc aatatcgcgt ggaccacgag gtaggtcttg gctccgtcaa acatgacgct
     1021 ttggtagcgt ttctggttct gcaccgtaat ttcgggcggc agcggcttct tgccttcggg
     1081 tgaccagctg agcggcatga accagatgcg tagcttgttg aagaggccag gtgctttcag
     1141 catttccctg aaaatcacga cgtagtagtg aaaattgatt ttgatcgggt taaacgactg
     1201 cgggtggtta tacatgccat agacaatttt ttcgcgctct tcagcaaagg tactgaacat
     1261 cctgtcccag atgatcagaa actcgccgaa attcttatcg aggtactggt agttggcgcc
     1321 atggtgcacg cggtggtgcg acggtgtggt gaagatgaac tcgatcggcc gccacagctt
     1381 gggaatgaat tctgtgtggt gcaagaaaga gatgaagaga tgcagcgccg tcatggtata
     1441 gatgtcgaag ggagtaaagc ccacgagggt cagcggccaa aagaaaaaga acgaaaacag
     1501 ccgttgcgtc acactcgcgc gcagggccac gaagaagttc atctcttcgg ctgagtggtg
     1561 cggcgaatgc gccgcccaca tgatcttcat ttcatggccc cagcggtgca cccagtaaaa
     1621 gatgaaatcg acgccgagca gcagcagaat gaagttcact ggtgtcattt cgagcggacc
     1681 ggcgacacgg tagttggcgt gtagccagcc atacacttcg tacacgcccg ctgccacgag
     1741 caggttggtg gtctggttac cgagcgcggt gccgaaattg gccactgcct cctggaagtt
     1801 gatgtacttg cgcttgaaga cgaagcaggc cgctatctcc agcagcacga agaagatcgc
     1861 gatcggtgtc agcagcgcat agacgttcat ggcccacaga acacctcgcc gggccgagcg
     1921 tcaatgcttt cgcgttacgc gttgtattct tctgcggcgg ccgcgattgc ggcaaactcc
     1981 gccttgagct gcttcaggta ctgcaggtca cgcgaggcgc ggtagagcat cacgcagccc
     2041 gtgtgcgcga caagtatgcg ccttgccacc tgtttggcag cggcggagtt ctcgtggtac
     2101 tggaggtagg cgcgcgcctg gaagcgcaac cagcgctcct gcacagaatt gagctgcggg
     2161 gtgaagagcg gccgctcgtc gagagagagc tcactcgcga acgcggtgaa ggggcagcca
     2221 aaaaactcgc cgcgctttgc cagcacaaag agtgcagtcg cccatgaaga caggtacttg
     2281 tcaaaagttg aactccgccc gagcaggtga tcgagcatac cgaaaagcat ctcagcacgg
     2341 tattccaggt aggcgaggcc cagcccggtc ttggattcga agtggtagta cagtgccggc
     2401 ttcttaatgc cgcacatctt cgtgatctcg tcgatgcccg tgttgttgta gccttgcgcg
     2461 taaaagagag cgccggcctt ttcgagaatc ttctgcttgg tatcgacttg cacgtttctt
     2521 caatgccctt gagggcctgc ctacgcaacc ccttgctgcg ggcgcgcgcg ttcgtaaaga
     2581 aactcatagc
//
LOCUS       DAOQJF010000426         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_28619_length_2587_cov_4.772512, whole genome shotgun sequence.
ACCESSION   DAOQJF010000426 DAOQJF010000000
VERSION     DAOQJF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28619_length_2587_cov_4.772512"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PLF85_16075"
     CDS             complement(<1..1069)
                     /locus_tag="PLF85_16075"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HNE21238.1"
                     /translation="MAQKKGKSPRKENTTAKLFAYPAKLRCEIAGKPNGKWHHVQFVT
                     QEEAKKLTGLPAFFKADAGETATQATGFVVGLGSLEKLDGETFADTVAAALAKAAQKL
                     EEYQVVVDRAVYDKVGFRTALRLLAVAQSNAEYPSDFLKGEKNRKKVIVKKTEIVVPQ
                     SFAAVAGEILASALVEARHINAMRQTQALPGNYLTPQTMDERAAVIAKQYKLTRKSFG
                     KAELARMGAGGIIAVSQGSDREPRLIVLEYRHPQAKKTLGLVGKGVTFDTGGISLKPG
                     ADMHEMKYDMSGSAMALHALAAIAEQKIPVNVICAIAMVENMPSGNAFKPGDVYTALN
                     GKTVEVQNTDAEGRLILADALT"
     gene            1181..1798
                     /locus_tag="PLF85_16080"
     CDS             1181..1798
                     /locus_tag="PLF85_16080"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNE21239.1"
                     /translation="MQSVRKLRRRQGFTLVELMVVLVIGGSLLLMAYNLLTGFMQAQL
                     SGNQDIALEGYFQDARRQALIQAKTLTLEINLDKKTFGLREYDPKLELNPDASLQQLA
                     EIKRYRIDRAVDTDREKEEKPKPKWVNPERKIPTVLTKILSSSGLELTGPILYIHFYP
                     TGNSDAVILQFGETNPNYVYIPRYNLQPVHLNQLTAFERQRVVRQ"
     gene            1795..2292
                     /locus_tag="PLF85_16085"
     CDS             1795..2292
                     /locus_tag="PLF85_16085"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNE21240.1"
                     /translation="MKFFYRFRRYARHRRRYGFTLIEVLMAMMLTASMTGLLISSVVN
                     AMSMRQKALSLGKAVTLAKKKMHEIKALNKTDSGQGEFPGMPGYRFAYTIREQELDLQ
                     KIADELGAGKEVANSAAAKYFKERNNSAKSATGLTFKLLHFHVEVSWSEKDKYALDYY
                     RGLGI"
     gene            2289..>2587
                     /locus_tag="PLF85_16090"
     CDS             2289..>2587
                     /locus_tag="PLF85_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNE21241.1"
                     /translation="MKIPRSQRGFTLVELLIVIGLAGFIFTTLMAVYLQVKKLAYEQT
                     RVSNQSAAAIEFGTAMSYDLANLVFEKYNIRQVFLVEKELHGGKRFDAFVFPSAAL"
BASE COUNT          577 a          725 c          695 g          590 t
ORIGIN      
        1 acgtcagtgc gtcagcgaga atcaggcgcc cctctgcgtc ggtattctgg acctcaaccg
       61 tcttaccgtt gagtgcagtg tagacatcgc ccggtttgaa agcattgccc gagggcatat
      121 tctctaccat cgcaatagcg cagatgacgt ttacgggaat cttttgttca gcaatcgccg
      181 cgagagcatg cagggccatc gcgctgcccg acatgtcgta tttcatctcg tgcatgtcgg
      241 cccctggctt cagcgaaata ccgccggtat cgaaggtgac tcctttgccg acgagcccca
      301 gcgtcttttt cgcctgcgga tgccggtatt ccagcacgat aagccgtggt tcgcggtcag
      361 acccctggct cacggcgatg attccgcccg cgcccatgcg tgccagttca gccttgccga
      421 aagatttgcg cgtgagttta tactgttttg caattaccgc ggcacgctca tccatagtct
      481 gcggcgtgag gtagttgccg ggcagtgcct gcgtctgccg cattgcgtta atgtggcggg
      541 cttctacgag ggcagaagcg aggatttccc cggcaaccgc ggcgaaactc tgcggcacaa
      601 caatttccgt ttttttgacg atgaccttct tcctgttttt ctcacccttg agaaaatctg
      661 aagggtattc agcattactc tgtgccactg cgagaagacg cagcgcggta cgaaaaccga
      721 ccttatcata aaccgcccgg tctactacta cctggtattc ttcgagcttc tgtgccgcct
      781 tagcaagggc agcggcgaca gtatcagcga acgtttcgcc atcgagtttt tcgagcgaac
      841 cgaggccgac gacgaagcct gtggcctggg tggcagtctc acctgcgtca gctttaaaga
      901 atgccggtag accagtgagt tttttggctt cttcctgcgt gacaaattgc acatggtgcc
      961 atttgccatt gggctttccg gctatttcac agcgaagttt tgccgggtat gcgaatagtt
     1021 ttgcggtagt gttctctttg cggggggatt tccccttttt ctgcgccatg gttttgtttt
     1081 gaaaaaatgc tcagcgcgga aagtaaaagg cggaaatcag ctcccataag acaggctaaa
     1141 acgcttaaca gcttattgcg ccggaagggt gctaccgcaa atgcagagcg tacgcaaact
     1201 gagacgccgg cagggtttca ccctggtgga gctcatggtc gtgctggtaa tcggtggctc
     1261 gctgctgcta atggcttaca acctgctgac cggcttcatg caggcgcagc tctcgggcaa
     1321 ccaggatatc gccctcgagg gctattttca ggatgcgcgc cggcaggcgc tgatacaagc
     1381 caaaacgctc acactcgaaa tcaacctcga caaaaagacc ttcggcctgc gcgagtacga
     1441 tccaaaactg gagctcaacc ccgatgcatc gctgcagcag ctcgctgaaa tcaagcgcta
     1501 ccgtattgac cgcgcggttg ataccgaccg ggaaaaagaa gagaagccaa aacccaaatg
     1561 ggtaaatccc gagcgcaaga tacccactgt gctcaccaaa atactcagca gctcgggtct
     1621 ggagctcacc ggcccgattc tctacatcca cttttatccg acgggcaact ctgacgcggt
     1681 gattctgcag ttcggtgaga ccaaccctaa ctacgtctac ataccgcgct acaacctgca
     1741 accggtgcac ctcaaccagc tgaccgcgtt tgaacgccag cgggtggtgc gccaatgaag
     1801 ttcttttacc ggttcaggcg ctacgcacgg caccggcggc gttatggttt taccctcatt
     1861 gaagtgctaa tggcgatgat gcttactgcc tcgatgacag gcctgctgat ttcgagcgtc
     1921 gtcaacgcga tgtccatgcg gcagaaggcg ctcagccttg gcaaggctgt cactctcgcg
     1981 aaaaagaaga tgcacgaaat taaggcgctc aacaagaccg atagcggcca gggggagttt
     2041 cccggcatgc ccggttaccg ctttgcttac actatccgcg aacaggagct cgatctgcag
     2101 aaaatcgcgg atgaactcgg cgctggcaaa gaagtcgcca acagcgcggc tgccaagtac
     2161 ttcaaagagc gtaataattc tgcgaagagc gcgacgggtc tgacgttcaa gctgctccac
     2221 tttcacgtcg aagtcagctg gagcgaaaaa gacaagtatg cgctcgacta ttaccggggt
     2281 ctcggcatat gaaaataccc cggagccagc gcggttttac gctcgttgaa ttactgattg
     2341 ttatcggcct cgccggtttt attttcacca cactgatggc agtttacctg caggtgaaaa
     2401 aacttgctta tgaacagacg cgcgtttcca accagtcggc cgccgcgatc gagttcggca
     2461 ccgcgatgag ctatgacctg gcgaacctgg ttttcgaaaa gtacaacatc cggcaggtat
     2521 ttctcgtcga aaaagaactg cacgggggca aacgcttcga tgcctttgtt ttcccatcgg
     2581 cggcgct
//
LOCUS       DAOQJF010000427         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_29300_length_2552_cov_3.670404, whole genome shotgun sequence.
ACCESSION   DAOQJF010000427 DAOQJF010000000
VERSION     DAOQJF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29300_length_2552_cov_3.670404"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PLF85_16095"
     CDS             complement(<1..666)
                     /locus_tag="PLF85_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNE21242.1"
                     /translation="MKISNPNMTELSAKAAADLSKVKYKQYGKYLGDFVVGETYEHPR
                     GMTVTAGMIQDFSTTFLEANPLYLNLEYAKALGHPAIPASPMLVFNLLLSLGVQNDSE
                     KAYANLGYYNVKFLKNVYPGDTITSFTKIVDKKERGEGKPGIVTINTIGNNQRGERVA
                     QYMRKIMVPPAPAGYQPPKFIPSKTDVPPYAETVEIEIPDFDAAKWPSTVTRPDTYYE
                     DFNV"
     gene            787..1026
                     /locus_tag="PLF85_16100"
     CDS             787..1026
                     /locus_tag="PLF85_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21243.1"
                     /translation="MKRLQYTVRNVTPEIDRGLRQTARKKRQSLNDTLLEALQASLGT
                     LENHDLDFVAGTWIEAPATDKALKDQRKIDKDMWS"
     gene            1027..1413
                     /locus_tag="PLF85_16105"
     CDS             1027..1413
                     /locus_tag="PLF85_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE21244.1"
                     /translation="MQLLLDTNTYTELARGNASALSKVQRATHILLTFVTLGELRAGF
                     AAGNKAQQNEKVLQKFLASSRVKVIYPDSETTHYYARIYAQLRKAGTPIPANDLWIAA
                     LSMQHGVPLYTLDAHFKKVESIDLLD"
     gene            1476..>2552
                     /locus_tag="PLF85_16110"
     CDS             1476..>2552
                     /locus_tag="PLF85_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE21245.1"
                     /translation="MTAAKATRIANLSDKDRGSKIIQWIRFGDRRLRRRHTWLNRFQD
                     QIGLAITLGSAGGMIVMSGLYIADLVPAWATIVTNGILASFLHEMEHDLIHSLYYKNS
                     QRMQNFLFWIVWLFRANTVNPWFRKEIHLLHHRVSGHKNDVEERYISNGMPWGFKRLL
                     VMLDPLAALTIQGPRIKRDALADLRRIKAPHKIIPLQRTFMLLWYSFLLLSLTRLGFF
                     LAGAEFAPPAWLAPIVTFLNTAAVIYLVPCWLRQSAIQIVSSNMHYYGSTGSEDRDPT
                     RVAPTTAVKIDSLVKQTQVLNSWLVLPLHIFCFNFGATHGIHHFVVNQPFYLRQWGAL
                     FVTKALRRYGVRFNDFASMRRANAF"
BASE COUNT          573 a          723 c          670 g          586 t
ORIGIN      
        1 gacgttgaaa tcttcgtaat aggtgtctgg ccgcgttacg gtcgaaggcc acttcgccgc
       61 atcaaaatcg gggatttcaa tctctactgt ttcggcgtac ggaggcacgt cagtcttcga
      121 aggaatgaat ttcggcggct ggtaaccagc aggtgcgggt ggtaccatga tcttgcgcat
      181 gtactgcgcc acacgctcac cgcgctggtt gttgccgata gtattgatgg tcacaatacc
      241 cggcttgcct tcgccgcgct cttttttgtc gacgatcttc gtgaatgaag taatggtatc
      301 gcccggatac acattcttca agaacttcac gttgtagtaa ccgaggttcg cgtacgcctt
      361 ttcggagtca ttctgcacgc cgagcgagag cagcaggttg aagacgagca ttgggctcgc
      421 gggtattgcc gggtggccca gcgccttcgc gtattcgagg tttaagtaaa gagggtttgc
      481 ttcgagaaag gtcgtcgaaa aatcctggat catacccgcg gtgacagtca taccgcgcgg
      541 gtgctcgtac gtctcgccga cgacgaaatc gcccaggtac ttgccgtact gcttgtactt
      601 cactttgctc aggtctgcag ccgctttggc tgacagctcg gtcatattgg gattgctgat
      661 tttcatgggg cagagtcgca gcaaaagtca ggctgaaaac cagaagtgct tggctgcctg
      721 cgacgccatg agcagcagaa acgcgattcg cttgacgtgc aggcaataat tgcctatact
      781 gtaggcatga agcgccttca atataccgtg cgcaacgtga ctcccgagat tgaccgtggc
      841 ctcaggcaga ctgcgcgcaa gaaacggcaa agcctcaatg acaccctact cgaagctttg
      901 caggcttcac tgggaactct ggaaaatcac gatctcgatt ttgtggcagg tacgtggata
      961 gaagcccccg ccacggacaa agccctgaaa gaccaacgta agatcgacaa ggacatgtgg
     1021 tcataaatgc agctgctgct agatacgaat acctatacag agctggcccg gggtaatgcg
     1081 tctgccttat ctaaagtaca gcgagccacg catatcctgc tgacctttgt taccttaggt
     1141 gagctgcggg cgggtttcgc agctggcaac aaggcacagc agaatgaaaa agtgctccaa
     1201 aaatttctcg cttcatcacg agttaaggtg atttaccctg atagtgagac cacccactac
     1261 tacgcgcgaa tatatgcaca gctgcgaaaa gcaggcaccc ccataccggc aaatgacctg
     1321 tggatagcgg cgctgtcaat gcagcatggc gtaccgctct acacactcga tgcacacttc
     1381 aaaaaggtcg aatcgatcga cctcctggat taaccgaaag ttgacaccag tttcattttt
     1441 gctttgcgcc gcaggccggt ccattatccg ttgccatgac cgcagccaag gccacacgca
     1501 tcgcgaacct cagcgataaa gatcgcggca gcaaaattat ccagtggatt cgcttcggcg
     1561 accgcaggtt gcgtcggcgg catacctggc ttaaccgctt ccaggaccaa atcggcctgg
     1621 ctatcacgct tggctctgcc ggcggtatga tcgtgatgtc ggggctatat atagcggatc
     1681 tcgtacccgc atgggccacg atcgtcacga acggtattct cgcttctttt ttacacgaga
     1741 tggaacacga cctgatccac tctctctact acaagaattc gcagcgcatg cagaacttcc
     1801 tgttctggat tgtctggttg ttccgcgcga acacggtcaa cccgtggttt cgcaaagaaa
     1861 tccacctgtt gcaccaccgc gtctcgggac acaagaacga cgtcgaagag cgttacatct
     1921 cgaacggtat gccgtggggc ttcaagcgcc tgctcgtcat gctcgacccg cttgcggcgc
     1981 tcaccataca ggggcccagg atcaagcgcg acgcgctggc cgacctgcga cgcatcaagg
     2041 ccccgcacaa gatcattcca ctgcaaagaa cttttatgct actctggtac agtttcctgc
     2101 tgctatctct caccaggttg ggattttttc ttgcgggcgc cgaattcgca ccgcctgcat
     2161 ggctggcccc catcgtgacg tttctgaata ctgcagcggt tatttacctg gtaccgtgct
     2221 ggctgcgaca gtccgcgata caaatcgtct cttcgaacat gcactactat ggttcgacag
     2281 gctctgaaga cagagacccg acgcgggtcg caccaaccac ggcggtaaag atcgactcgc
     2341 tcgtgaaaca gacgcaggtt ttgaactcat ggctcgtatt gccgctgcac atcttttgct
     2401 ttaacttcgg cgccacgcac ggcattcacc acttcgtcgt gaaccagccc ttttacctca
     2461 ggcaatgggg cgccctgttt gtcaccaagg ccctgcgccg ctatggcgtg cgcttcaatg
     2521 atttcgccag catgcgccgc gccaacgcgt tt
//
LOCUS       DAOQJF010000428         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_29319_length_2551_cov_4.895433, whole genome shotgun sequence.
ACCESSION   DAOQJF010000428 DAOQJF010000000
VERSION     DAOQJF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29319_length_2551_cov_4.895433"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..2439
                     /locus_tag="PLF85_16115"
     CDS             34..2439
                     /locus_tag="PLF85_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21246.1"
                     /translation="MSSVWPENEQARLEALRSYQILDTLPEASFDRLTRLASAICGVP
                     IALVSLIDTDRQWFKSRVGLDAAETARNISFCQHAILGTGIMEVEDATRDDRFRANPL
                     VTEDPEIRFYAGAPLIDPDGFALGTLCVIDRQPRSLTQEQRTALRDLADEVMEHIRVR
                     KERNTHSEARARLSSMIAALPDILFIFDNTGKFLDCHAGEGTRLWMQPKDFIGRRVTE
                     IMPAEFAETVMARIRETQAKRGLVTFRYETPGAFHGENEYFEARMVLTEQDHILCVIR
                     DVTTETRKTRKLAHAQQLLTLTGRVARLGAWEYWPQHDRLHLSDVFCELLEISPNEQV
                     KMAEAMPRFVAKPNVEAVQSAISSCITEGKPFDLELPIVNAKGRNFWVRIQGAANFAN
                     GVCTSIHGTLQDIDSMKQVEVALDSARKQAEAANQAKSDFLSNMSHELRTPLNSILGF
                     SQLLEMNTNRHLDSEDLENVGHIRKAGSHLLNLISEILDLSRIESGNMDLVFEYFRMG
                     EILEEAVVFVKPMAQRRDVGIEIIPQHEYTVHADRIRLKQVLLNLLTNAIKYNRTGGF
                     VRIDYASAGTGYLRVSVRDSGPGIAAAQIPHLFQPFQRLGAETSTIEGTGIGLTITRR
                     LMDLMHGDVFLRETSTDGSEFCIDVPLAAGPMTPIQRAIQVAGTAKNYISGTVLCVED
                     NADSLSLMEHFFRRMEHITLLTAKSGDQALELAHRTPVDLVLLDIHLPANSGYQVLRR
                     LKSQPLTASVPVVAVSAAATRDDIERGRSAGFDEYLTKPVRLDRLEEIVIKYCHRHRS
                     PRA"
BASE COUNT          583 a          696 c          709 g          563 t
ORIGIN      
        1 gcccgcggtc tcatcgctga gcggtagtca ataatgtctt ccgtctggcc agaaaacgaa
       61 caggcgcgtc tcgaggccct gcgcagttac cagattctcg atacgctgcc tgaagccagt
      121 tttgacaggc tcacccggct ggcttcggcg atatgcggag tgccgattgc actcgtgagc
      181 ctgatcgata ctgaccggca atggttcaag tcgcgggttg gactggacgc ggccgaaacg
      241 gcgcgcaata tttcgttctg ccagcatgct attctcggta caggaatcat ggaggtcgaa
      301 gatgcgacgc gggacgaccg atttcgcgcg aaccccctcg ttacggaaga tcccgaaatc
      361 cgcttttatg ccggcgcccc actgattgat ccggatggtt ttgcgctcgg cacgctctgt
      421 gtcattgacc ggcaaccccg cagcctgacc caggagcagc gcacagctct gagagacctg
      481 gctgacgaag tcatggagca tatacgcgtg cgcaaagagc gcaataccca cagcgaggcc
      541 cgtgccaggc tcagctcgat gattgccgcg ttacccgata tcctttttat ttttgataat
      601 acaggaaagt ttctcgattg ccatgccggg gaagggaccc gcctctggat gcaaccaaaa
      661 gatttcattg gccgccgcgt aacggagatc atgccagcgg aatttgccga aacggtgatg
      721 gccaggattc gcgaaaccca ggcaaagcgt ggattggtaa cttttcgtta cgaaactcca
      781 ggcgcctttc acggggagaa cgaatacttc gaggcgcgca tggtgttaac ggagcaggat
      841 catatcctct gcgtgattcg cgacgtgacg actgaaaccc gaaagacccg caagcttgcg
      901 cacgcccagc agctactcac tctgaccggg cgcgttgcgc gcctcggagc gtgggagtac
      961 tggccgcagc atgatcgcct gcacctgtcc gatgttttct gcgagttgct cgaaatatca
     1021 cccaatgaac aggtgaagat ggcagaagca atgccacgat ttgttgcgaa gcctaatgtt
     1081 gaagccgtgc aatcggcgat cagttcctgc attacagaag gaaaaccttt tgatctggag
     1141 ctgcccattg taaacgcaaa ggggcgaaat ttttgggtgc gcatacaggg cgcggcaaac
     1201 ttcgccaatg gcgtctgcac aagtattcac ggtacgttgc aggatatcga cagtatgaag
     1261 caggtggagg tcgcgctgga ttctgcgcgt aagcaggctg aagcggccaa ccaggcaaaa
     1321 tcggatttcc tctccaatat gagtcatgag ctgcgcactc cgctcaactc gattttgggc
     1381 ttcagccagt tgctggaaat gaataccaac cgccaccttg acagtgaaga cctggagaat
     1441 gttggccata ttcgtaaagc aggttcacac ctgctgaacc tcatcagtga gattcttgac
     1501 ctgtcgcgca tcgaaagtgg caacatggac ctcgtatttg agtattttcg tatgggcgaa
     1561 attcttgaag aagccgtggt ctttgtaaag cctatggctc agcgccgtga cgtcggtata
     1621 gaaattattc cgcagcacga atacaccgtg cacgccgaca gaatcaggct gaagcaggtg
     1681 ctgctcaatc tgctcaccaa tgcaataaaa tataaccgta ccggcggttt tgtgcgcatc
     1741 gattatgcct cggcaggcac gggatacctg cgcgtttcag tcagggacag cgggccgggt
     1801 attgctgccg cgcagatacc gcacctgttt cagccattcc agagactggg agcggagact
     1861 tccactatcg aaggcactgg aattggtctt acgattacgc gccggctaat ggacctcatg
     1921 catggcgatg tattcctgag agaaacctcg actgacggca gcgaattctg catagatgtt
     1981 ccgctggcag ccggcccaat gaccccgatc cagcgtgcaa ttcaggttgc cggcactgca
     2041 aaaaattata tttccggtac agtcctgtgc gttgaagata atgccgacag tctcagtctc
     2101 atggagcatt ttttcaggcg catggagcac atcacgctgc tcacggcaaa gtcaggagac
     2161 caggcgctcg agctggctca tcgtacgccc gttgaccttg tgcttttgga tattcacctg
     2221 cccgcaaact cgggctatca agttctgcgg cgcctgaagt ctcagccgct gactgcgtca
     2281 gtaccggtcg tcgctgtttc ggccgcggct accagggacg atatcgagcg cggcaggtca
     2341 gccgggttcg acgagtatct caccaagcct gtgcgccttg atcgtctcga agaaatcgtc
     2401 atcaagtact gtcatcgtca ccgctcgcct cgtgcttagg tcccaattag cttgtcaccg
     2461 ctaagcagag tgtcaggctt gcctcaggca tgaccgacga aatctggaag tttatctcaa
     2521 gcgccaacga agctgatttg caggcggagc t
//
LOCUS       DAOQJF010000429         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_29727_length_2530_cov_4.144242, whole genome shotgun sequence.
ACCESSION   DAOQJF010000429 DAOQJF010000000
VERSION     DAOQJF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29727_length_2530_cov_4.144242"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PLF85_16120"
     CDS             <1..1308
                     /locus_tag="PLF85_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE21247.1"
                     /translation="KEQVLKELKEMQAAEIKKWKEGYVSGAVYHGDDSHIEYLNQAYA
                     IHSQSNPLHTDLWPSASKFEAEIISMAGRMLSEGASESVRAELCGSVTSGGSESILLA
                     MKTYRDMAYAERGVRNPEMVAPVTAHAAFDKAAQYFKIKLRKVPVDSSFRADLDAVKK
                     AINSNTVVVIGSAPAFPHGTVDPIEEMSELARSKKIPFHTDACLGGFVLPFARKLGYP
                     VPKFDFNLPGVTSISVDTHKYGYAAKGTSVILYRNPQIRHHQFFTVTNWPGGMYFSPT
                     FAGSRPGGLSAACWAALTSIGEKGYLEAAKKILGAADYIKKEITKIPELKLLGDPLYV
                     IAFASDSLDIYKVMEHMTHKGYSLNGLHRPACVHIALTLRHAEPGVKERFIEDLKEAV
                     AHVKANPSEKGSSAPIYGMAATLPVRSVVGDLLKTYMDAYYKV"
     gene            complement(1472..2212)
                     /locus_tag="PLF85_16125"
                     /pseudo
     CDS             complement(1472..2212)
                     /locus_tag="PLF85_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(2288..>2530)
                     /locus_tag="PLF85_16130"
     CDS             complement(2288..>2530)
                     /locus_tag="PLF85_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21248.1"
                     /translation="MDAAAVERWDALDSERENRWKSGKEYLAEREKLLKNSSGDSTEA
                     LNELRKKYFGAEADSIAAEEAEGHLRFQGEQRIGID"
BASE COUNT          579 a          717 c          680 g          554 t
ORIGIN      
        1 aaagaacagg tcctcaaaga actcaaagaa atgcaggcag ccgaaataaa aaaatggaaa
       61 gagggttatg tttccggcgc ggtgtaccat ggcgatgata gccatatcga atacctcaac
      121 caggcgtacg caatccattc gcagtcgaac ccgctgcaca ccgacctgtg gccgagtgcc
      181 agcaagttcg aagccgagat catatcgatg gccggacgca tgctctctga aggcgcgagt
      241 gagtctgtcc gcgcagagct ctgtggttcg gtgacttcag gcggcagcga aagtatcctg
      301 ctcgcgatga agacctaccg cgatatggcc tacgccgagc gcggcgtccg caaccctgag
      361 atggtagcac ccgtcacggc ccacgccgct tttgataaag cagctcagta cttcaagatc
      421 aagctgcgca aagtgccagt cgacagctcg ttccgcgccg acctcgatgc ggtcaaaaag
      481 gcgattaact cgaacacagt tgtggttatc ggttccgcgc ctgcattccc gcacggcacc
      541 gttgacccga tcgaagagat gtcagagctc gcccgctcca aaaaaatccc attccatacc
      601 gatgcctgcc tcggtggttt tgtgctgccg ttcgcgcgca aactcggcta cccggtgccg
      661 aaattcgatt tcaacctgcc gggagtcacg tcgatttcgg tcgatacgca caagtacggt
      721 tatgcggcga aaggtacctc ggttatcctt taccgcaatc cacagattcg ccaccaccag
      781 ttctttacgg tgaccaactg gccgggtgga atgtatttct ctccgacatt tgccggctcg
      841 cgtccgggcg ggctctctgc tgcctgctgg gcggcgctca cctcgatcgg cgaaaagggt
      901 tatcttgaag cggcaaaaaa gatactcggc gctgcagact atataaagaa agaaattacc
      961 aaaatacccg aactcaagct gctcggtgat ccgctctacg tgattgcctt tgcctctgat
     1021 agtctcgaca tctacaaggt aatggagcac atgacccata agggttattc gctcaacggc
     1081 ctgcaccgcc cggcgtgtgt gcatattgcc ctcacgctgc gccacgcaga gcccggcgtg
     1141 aaagagcgct tcatcgaaga cctgaaagag gccgtcgcgc atgtgaaggc caacccatca
     1201 gaaaagggga gctccgcgcc gatttatggt atggctgcca cgctgccagt gcgcagtgtc
     1261 gtgggcgacc tcttgaaaac ctatatggat gcctattaca aggtttgatt gatttgcttt
     1321 tggctatggg cttaagccca tagccaaaag acagactctc gccagcttcc agcaaaaaga
     1381 ttctgtcgct ggctcctgag attgcaatag agtctttaat catttccatc tcgcgtccgc
     1441 cgcgccggta gaacgtgctg tggtgaatgg gtatcatgta acgcgcgccg cagtcgcgga
     1501 aaatctgtag ggcgccgtac gggttgacat gcaccgcgtt gcctaaaccg ctgccggtgc
     1561 cgaagccacc tgaaggccca acgggaatca gcgccacgtc gatcttgaag tgtttacgca
     1621 gctcgcggaa atgttcttca tggtagccgg tgtcgcctgc gaaaaaaacg gtgatgccct
     1681 tgtaacggat gatatagccg ttgtacggtt gtccatccca gacattgtcg acgagcatac
     1741 gcccgccaaa gtgcgctacc ggcacgacaa aaatttcgag accgtcttct tcaagcgagt
     1801 aacctggtgc aacgcggtgc tcgtggtgcg ctaatcctcc cggtatatac ataaacccgc
     1861 tcgccggcgc gaagaggtgc cttgtccctt tgagcatctc tatcgaaggc acatcgaggt
     1921 ggtcaaaatg attgtgggag attacaatcg catcgaccgg cttcagtgag gcggtatcga
     1981 ttccgacttc tgtcaggcgc ctgacgacgc cgccggtacg cggcgaaaaa ttcgggtccg
     2041 tgatgatcca cttgtcgtgt aatcgcatga gtaccgtagc atggccgacc caccagaatt
     2101 cggcatcgcc acgctgcgtt gaaatctgcg gtgcgggttt tgctgtcttt atagtgctgg
     2161 aaaaattacc gcagccaatg accaacagca gaagtagaaa aaagttgcgc accgggctta
     2221 agatagcaaa acccggcgaa attgaccact caacccattg ctggctgcca aaaagacggc
     2281 cggtcaatca atcgatgccg atgcgttgct cgccctggaa acgaaggtgg ccttctgctt
     2341 cttcggcagc gatgctgtct gcttctgcgc caaagtattt cttgcgcagc tcattcagcg
     2401 cctcggtact atcgcccgaa gaattcttga gtagcttttc gcgctcagcg aggtattctt
     2461 tgcccgattt ccagcggttt tcccgctcgc tgtcgagtgc atcccagcgt tcgacggctg
     2521 cggcgtccat
//
LOCUS       DAOQJF010000430         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_29767_length_2528_cov_4.537000, whole genome shotgun sequence.
ACCESSION   DAOQJF010000430 DAOQJF010000000
VERSION     DAOQJF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29767_length_2528_cov_4.537000"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2225)
                     /locus_tag="PLF85_16135"
     CDS             complement(<1..2225)
                     /locus_tag="PLF85_16135"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNE21249.1"
                     /translation="MRALNLSNRLLLTPLLAINASLSAAELSIVTNTGEPVEFGTAII
                     VEAKFKTNFENGRLTLPGMKQGTYSLRLTAPGYVSLEERLQLPLKKPLTLVMQLQTRR
                     AQKVRLITHEEKHNVSKHQLQQHELKSVPATFGDNISALATLPGVNRPGGIFGPLIIR
                     GAPDTANRYFIDDIPVINPQHFGGFQSVISNELIDDITLFSSAFPAYYGQALGAVIDI
                     RTIEAVAKPTATIQTGIISSNAFFAAPWSVFSPKLTHPSENASEAERQAYQDAKRKSE
                     VDTGFFSISGRVGYLSLLVPPIYKFITGKEIIAVPEYYDYQWKARWYLGDHNRHAVSM
                     FVFGSYDTFTLVRKLSDEEKKQRIEQGENPAAGDLTVFNDISSHSQSVSYDYLPSQNL
                     KIRTILFNTLIYSRFYREAPGRALGTIDVNTRPNITGLREIFDIWYMNRKARLKAGID
                     YQLFYFNSSGVTQQRKETIPQGQTDFNNPDHFDVISVAALGQNHLVSAYADNRLELGG
                     FFITPGIRSDYLARSGEATLDLRGLAGYKFPTNTTIAFSGGLYSSFAQTNTYRFNQAS
                     RRDARVVTADYLGSEKAVHRSMALEQEFGFHVFKVEGFYNNLYDMMQSTSSADQARGI
                     YFANTGGSVARGVEVSYRKSLQENEDGFFGWVSYTYTRADVSGVAGTFPFRFEQRHVV
                     KMIGVARLGAWELGARFELFTGFPYTPIMGSTCTPGYTCDGNPSTTLYTPVYSTDINS
                     D"
BASE COUNT          551 a          650 c          755 g          572 t
ORIGIN      
        1 tgatcgctgt tgatatccgt cgaatagaca ggggtgtaaa gtgtcgtcga ggggttgcca
       61 tcgcaggtgt accctggcgt acaggtgctg cccataatcg gtgtataagg aaaaccggtg
      121 aaaagttcga agcgcgcccc gagctcccac gcgcccaggc gtgctacacc aatcattttc
      181 acaacgtggc gctgttcaaa acgaaaggga aatgtgccag cgacacccga tacatcggca
      241 cgtgtgtacg tgtaactgac ccagccgaaa aagccgtctt cattttcctg caatgacttg
      301 cggtagctga cctcgacgcc gcgtgccacc gagccgccgg tattagcaaa gtagatcccg
      361 cgtgcctggt cggcagaaga agttgactgc atcatgtcgt agaggttgtt atagaagcct
      421 tcgaccttga acacatgaaa gccgaattcc tgctcgagcg ccatgctgcg atgcacggcc
      481 ttttcgctgc ccaggtagtc ggcagtgacg acgcgtgcat cgcgccggct ggcctggtta
      541 aaacggtagg tattcgtttg tgcaaacgat gaatacaggc cgcccgaaaa agcgattgtt
      601 gtattggtgg gaaatttata cccagcaagg ccacgcaagt caagagtcgc ttcgccggaa
      661 cgtgcaaggt aatccgatcg gataccaggc gtaatgaaaa aaccaccgag ctcgaggcgg
      721 ttgtccgcgt aggcagagac caggtgattc tgccccagcg ccgcgacgct gataacgtcg
      781 aagtggtcgg gattgttgaa gtcggtctgc ccctgtggta tagtctcttt acgttgttgt
      841 gtcacacccg aactattaaa gtagaacagc tggtagtcta tacctgcctt gagccgggcc
      901 ttccggttca tgtaccagat gtcgaagatc tcgcgcagcc ccgtgatgtt tggccgcgtg
      961 ttaacgtcga tggtgccaag agcgcgcccg ggcgcttcgc ggtaaaagcg gctgtagatc
     1021 agggtgttga acagtatggt gcgtatctta agattctgcg agggcaggta gtcgtatgag
     1081 acagactggc tgtgcgaaga gatgtcgttg aacacagtga gatcacccgc ggcggggttc
     1141 tctccctgtt caatgcgctg ttttttttct tcgtcgctca gtttacgtac cagcgtaaag
     1201 gtatcgtaac tgccgaagac aaacatcgaa accgcgtggc ggttatgatc gccgaggtac
     1261 caccgcgcct tccactggta gtcgtagtac tcgggtaccg cgataatttc tttgcctgta
     1321 atgaatttgt agatgggggg gacgagcagc gagagataac ccacacggcc tgagatcgaa
     1381 aagaaacctg tatcaacttc tgatttccgt ttcgcgtcct ggtatgcctg gcgctccgcc
     1441 tcgctcgcgt tttctgacgg gtgagttaac ttcggggaga aaactgacca gggcgcagca
     1501 aagaatgcat tcgaagaaat gatgccggtc tgtatggtcg cggtcggctt cgcaaccgcc
     1561 tcgatcgtgc ggatgtcgat gaccgcgccg agcgcctggc cgtagtaggc aggaaaagca
     1621 gaagagaaga gggttatgtc atcgatgagt tcattcgaga taaccgattg aaatcccccg
     1681 aagtgctgcg ggttgatcac gggaatgtca tcgatgaagt agcggttcgc tgtatcgggt
     1741 gcgccacgaa taatcagcgg accgaaaatg ccgccgggac ggttgacgcc cggcaatgtc
     1801 gccagcgccg agatgttgtc gccgaaggtt gccggcaccg acttgagttc gtgctgctgc
     1861 agctggtgct tgctgacgtt atgtttctct tcgtgcgtga tgagccgcac cttctgcgct
     1921 cgccgtgtct gcagctgcat gacgagcgtg agtggctttt tcagcggcag ctgcaggcgc
     1981 tcttcgagac tgacgtaacc cggggctgtc aggcgcagcg agtaggtacc ctgtttcatg
     2041 ccgggcagcg tgagacgacc attctcgaaa ttggtcttga attttgcctc gacaataata
     2101 gcggtgccaa actcgacggg ttcgccggtg ttggtgacaa tactcagctc agctgcagag
     2161 agactggcat tgattgccag cagcggtgtt agcaaaagcc tgtttgacag attcagtgcg
     2221 cgcaccttgt tgccagctcg aaagcgtcag cggcggctgc gagtgaatat tcaggaaagg
     2281 ttgggcagcg ctgtgtcagc ctgaacctgt cgaaaggtga cacgccgccg cacttcgact
     2341 agctcagtgc gacgctttac aagtcactgt gagttgccct gctactcgcc cctgccaaag
     2401 aagcgcatga ccagctcatt gccgtgcgcc acgaagaaca ccgtatcgcc atcatggata
     2461 tcagcgattt gccgcctggc tgcgggaaca gtgacctgtg ccttggcggt gatgtgtgat
     2521 atggttgc
//
LOCUS       DAOQJF010000431         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_30039_length_2515_cov_4.722358, whole genome shotgun sequence.
ACCESSION   DAOQJF010000431 DAOQJF010000000
VERSION     DAOQJF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30039_length_2515_cov_4.722358"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLF85_16140"
     CDS             complement(<1..585)
                     /locus_tag="PLF85_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE21250.1"
                     /translation="MKNFQGRVAVITGAGSGIGRALALNLAEKGCKLALSDIRLESVQ
                     ETAKLCERFTRDVMVDALDVADKAAMQAYPEKVVKQFGGANIVINNAGVAVSATVEET
                     SIEDYEWLMGINWWGVLYGTKFFLPYLQKADEAHIVNISSVFGIIGVPTQSAYNAAKF
                     AVRGFTESLRQELVDTHIGVSCVHPGGIKTNIAAY"
     gene            752..1450
                     /locus_tag="PLF85_16145"
     CDS             752..1450
                     /locus_tag="PLF85_16145"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE21251.1"
                     /translation="MAKYLVFAASSGIGQAVVEQLKADGHEAITTARSAAKIKPDFTV
                     DASDFDAVEYVFEQVGQIDGVVNCAGSLLLKPAHLTTAGQFDEVMVASLKTAFGVVRS
                     AGKYMRGGGSVVLVSSAAAMAGYANHEAIASAKAGIIGLVRAAAATYASSNLRFNAVA
                     PGLTETPLTEQLTSNPAARKVSEAMHALGRIGSPEDIARAICFLLDTRNSFITGQVIA
                     VDGGLSMVHPKVRA"
     gene            complement(1550..2056)
                     /locus_tag="PLF85_16150"
     CDS             complement(1550..2056)
                     /locus_tag="PLF85_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21252.1"
                     /translation="MQAETVQGWVFCPLLRHSDMSLTKLPDEKFIKKIFEAFAKGGPQ
                     EVQKYTAGVFHYIEALQKLPLTAEQRELGKKDISADYLRILDEDRKISEQVYAQGKSE
                     NIDWNKATIVNTMVLQTQKSEIAGWWLHKQLGVQFKSNGVAYKLNLDVIEINGDKFFV
                     RVSELLKQ"
BASE COUNT          542 a          682 c          671 g          620 t
ORIGIN      
        1 gtacgccgcg atgttcgttt taattccgcc ggggtgaacg cagctgacgc cgatatgcgt
       61 gtcgaccagt tcttgtcgca gtgattcggt aaaaccgcgc accgcaaatt tcgccgcgtt
      121 gtatgctgac tgcgtcggca caccgatgat gccgaataca ctcgagatat tcacgatgtg
      181 tgcttcgtcg gccttttgca ggtagggtag aaaaaatttc gtaccataga gaactcccca
      241 ccagttgatg cccatgagcc actcgtagtc ttcaatgctc gtttcttcga cggtagccga
      301 aacagccacg ccggcattgt tgataacgat gttagctcca ccgaactgct ttacgacctt
      361 ttcgggatat gcctgcatcg ccgccttgtc ggccacgtcg agcgcatcga ccatcacgtc
      421 gcgcgtgaag cgttcgcaga gcttcgcagt ttcctgcacg ctttcgaggc gaatatcgct
      481 gagcgcgagc ttgcagccct tctcggcgag attaagcgca agggcgcggc caatgcccga
      541 gcctgcacca gtgatgacgg ccacccggcc ctggaagttc ttcatggcgg atgtttctcg
      601 cttctgcgac tgggcgcaaa gtcaaaagtg acactttgtt caggtttgcg ctgtctgatc
      661 accttgggcc gagtttgacc tctcttaata ttgtccaagg ttccaatcaa aggtgataca
      721 cgataggggg tttctgtgct acttttcccg tatggcaaaa tatctcgtct tcgctgcctc
      781 aagcggcatc gggcaggctg tcgtcgagca gctaaaagcc gatgggcatg aggccatcac
      841 gaccgcccgc agtgcggcaa aaatcaaacc ggatttcacg gtcgacgcgt ctgatttcga
      901 cgcggtcgaa tatgtcttcg agcaggtcgg ccagatagac ggcgtagtca actgcgcggg
      961 ctcgctgctg ttgaagccgg cacacctgac gactgcgggg cagtttgacg aagtgatggt
     1021 cgcatcgctc aagactgcct tcggtgtcgt gcgctccgcg ggcaagtaca tgcgcggcgg
     1081 aggttctgtg gttctggtct cttcggccgc agcgatggca ggctacgcca accacgaagc
     1141 gattgccagc gctaaggcgg gtattattgg cctcgtgcgc gctgccgcag ccacctatgc
     1201 ttcgagcaac ctgcgcttca acgcggttgc accgggccta accgaaaccc cgctgactga
     1261 gcagctgact tcgaacccgg cagcacgcaa ggtctcagaa gcgatgcatg ctctgggtag
     1321 aatcggctcc cccgaagaca tcgcgcgggc gatctgcttt ttactcgaca cgcgtaacag
     1381 tttcattaca gggcaggtga tcgccgtcga cggcggattg agcatggtgc acccaaaggt
     1441 acgggcgtag caatgggaaa ccgcgtacct ttgacgattg cggttgccgc aaagtgcttg
     1501 ggacgatagt gggattgtcg ggatcccgca gtttttgaac tctgcttgat tattgtttca
     1561 atagctcact tacccggaca aaaaacttgt ccccgttgat ctcgatgaca tcgagattga
     1621 gcttgtaggc cacgccgttc gacttaaact gtacacctaa ttgtttgtgt agccaccaac
     1681 cggcaatttc ggatttttgc gtttgcagga ccatcgtatt cacaatggtt gctttgttcc
     1741 aatcaatgtt ttcagatttt ccctgtgcgt aaacctgctc agaaattttt cgatcttcgt
     1801 cgagaatgcg caaatagtca gcactgatat cctttttacc cagctcacgc tgttctgcgg
     1861 tgagagggag cttttggagc gcctcaatgt agtggaaaac acctgctgtg tatttctgca
     1921 cttcttgcgg gccgcctttg gcaaaagctt caaagatctt ctttatgaac ttttcatcag
     1981 gcaattttgt caggctcata tcagaatgcc ggagcagggg acaaaaaacc cagccttgga
     2041 cagtttctgc ctgcacctgg caccaatgtc cggtgacgcc gtaaatcgtc tctacggggc
     2101 catacaggtt aaatacattg atccgggagc cgaggggcag gttcgcgagc agtttgcccg
     2161 tctgaccggg ggtttccctt acgttagcgc cgacagtagc atcgattgca taaaaagatg
     2221 aatcgcctga gtttggtttc ttctggcagg cagctaccag agtcaggata agaaaagccg
     2281 ttagaaaaac tttcatattg tatcagactg atgacagcct catatccagt aaagcgccat
     2341 tttctccttt ttgacttttt gcgtttggga cagccgatgc agcatttttc cacatttcgt
     2401 cagagtaatt ccttcgccgt cacccggtgg cgatgctcaa acagccgctc aagctcgcgc
     2461 cgcgcaaacc atccaccgaa aaaagtaccc acgggtccga gcggcagtgc atatt
//
LOCUS       DAOQJF010000432         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_30530_length_2493_cov_4.726415, whole genome shotgun sequence.
ACCESSION   DAOQJF010000432 DAOQJF010000000
VERSION     DAOQJF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30530_length_2493_cov_4.726415"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..927)
                     /locus_tag="PLF85_16155"
     CDS             complement(55..927)
                     /locus_tag="PLF85_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE21253.1"
                     /translation="MAGTNKFKSFWANRYYRYGSMALVLLAFLLLFYRKPPVPTEIHR
                     VAKGTYEASIEEDGVTRVKEKFTVFSPAAGVMMRVEKHAGDTVRKGEILTHVMLDYMR
                     PVKSPIAGTILKVYRESEGPIDMGNPILDLGDTRELEIVSEVLTRDVVGLKPGNEVVI
                     SGWGGGTLAGKIRLIEPAAFTKISTLGVEEQRIRVIVDFSKPAEMGEGFQVRCRLVAK
                     RRQDSLTLPVGALFRSGEDWALYKVVKQRAVKATVKIAETSGSTALVESGVSEGDEVI
                     LFPGEKIKEGVKVK"
     gene            complement(928..>2493)
                     /locus_tag="PLF85_16160"
     CDS             complement(928..>2493)
                     /locus_tag="PLF85_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE21254.1"
                     /translation="TNAFFLPLVFLGVAAFLLHIVATRLIARQREQIASLMALGYTNR
                     EIAVHYLKLMLVISGSGALLGVGVGWYLGRSMTWLYGEFYRFPDLTHVFNLSVALSGL
                     VIGASAGVFGTWFSVKRVMRMQPAQAMRPPVPESFAQSFFERYFKNLPAITRMFLRNL
                     LRRPARTILTILGLSTAVMIMVLGLFMTDVMDSMLSTQFELLNRESMTVSFLRPVAAT
                     ALDELRSQPGIQLAEGYRQVPVRLRHSQFTKELAITGIPSDAELRRVANERGEVIVLP
                     PEGVLLNLRIAQKMGIRAGDRIELELLEGNRRKIRVVVANLVDEILGQGCYMRIDALS
                     RLLGEAHSINVAALRTDAARDDDIIAHLKESSQVASISTRAGTLKIFYEMMSRSMLAM
                     ITIIMIFASAISIGVVYNTAMILLSERTFELGSLRILGFTKGEVFEMIASELGTTILA
                     SLVPGCALGYFFAWLLMNTVDTEEFAIKLIITSRTYVTAMLVSLATAVISFVILYIRI
                     RTMDLVSVLKVRE"
BASE COUNT          556 a          759 c          670 g          508 t
ORIGIN      
        1 tatatcggat tctcccctct ccacggcgtg gggaggggtt ggggctgggg ttattcactt
       61 caccttcacg ccttctttga tcttttcacc cggaaacagg atcacttcgt cgccttcgct
      121 cacacccgac tccacgagcg cggtgctacc gctcgtctct gcgatcttca cggtcgcttt
      181 cactgcgcgc tgtttcacaa ccttgtagag cgcccaatct tcacccgaac ggaacagtgc
      241 accgaccggc aacgtcaggc tatcctggcg gcgttttgcc accagccggc agcgcacctg
      301 gaacccctcg cccatttccg cgggcttcga aaaatcgaca atcaccctga tgcgctgctc
      361 ttcgacaccg agcgtcgaaa ttttcgtgaa ggccgccggc tcaatcaggc ggattttacc
      421 cgcgagcgtg ccaccacccc aaccgctaat gaccacctcg ttacccggct tgagccccac
      481 gacatcgcgc gtcagcacct ctgaaacaat ctcaagctcg cgcgtatcgc cgagatccag
      541 gatgggattt cccatatcaa tcggaccttc actctcgcgg tacaccttga gaatcgtacc
      601 ggcaatcggc gacttgaccg gccgcatgta gtcgagcatc acgtgcgtca gaatttcacc
      661 cttgcggaca gtatcgccgg catgcttttc gacgcgcatc atcaccccgg ctgccggcga
      721 aaagaccgtg aacttctctt tgacccgtgt tacgccgtct tcttcgatcg acgcttcgta
      781 ggtccctttg gccacgcggt gtatttcagt cggcacgggc ggcttgcggt agaaaagcag
      841 caagaaagcc agcaacacca gcgccatact gccgtagcgg tagtagcggt ttgcccagaa
      901 agatttgaat ttgttggtgc ctgccatcta ttccctcact tttagtaccg atacgagatc
      961 catagtcctt atacgaatat agagtatgac gaaactgata acagccgtcg ccagcgagac
     1021 cagcatggcg gtaacgtagg tacggctggt gatgatcagc ttgatagcaa attcttcagt
     1081 gtcgacagtg ttcatcagca gccaggcgaa gaagtaaccc agcgcacagc ccggcaccag
     1141 cgatgcaagt atggtcgtac cgagttctga ggcgatcatc tcaaatacct cgcccttggt
     1201 aaaaccgaga atgcgcaggc tgcccagctc gaatgtccgt tccgaaagca atatcatcgc
     1261 cgtattgtac acaacaccga tcgaaattgc cgaggcgaag atcatgatga tggtgatcat
     1321 cgcgagcatg ctgcgcgaca tcatttcgta gaagattttg agcgtgcctg cgcgcgtgct
     1381 gatcgatgcc acctgcgacg actctttcag gtgcgcgatg atgtcgtcgt cccgtgccgc
     1441 gtccgtccgc agcgccgcga cgttgatgct gtgcgcttca ccgagcaggc ggctcagcgc
     1501 gtcgatgcgc atgtaacaac cctggccgag aatctcgtcc accaggtttg ccaccacgac
     1561 gcgtatcttg cggcggttac cctcgagtag ttcgagttcg atcctatcac ccgcgcgaat
     1621 gcccattttc tgcgcaatac gcaggttcag cagaactccc tcggggggta acacgatcac
     1681 ttcgccgcgc tcattggcga cgcgccgcaa ctcggcatcg ctgggaatac ccgtgatcgc
     1741 tagctctttc gtgaactggc tgtgtcgcag acgcacgggc acctgccggt aaccttcggc
     1801 cagctgtatg cccggctggc tgcgcagctc gtcgagtgcg gtagcggcaa ccggccgcag
     1861 aaacgatacc gtcatacttt cacggttcag cagttcaaac tgcgtcgaga gcatcgaatc
     1921 catgacgtcg gtcatgaaga gacccagtac catgatcatg accgcagtcg aaaggccgag
     1981 tattgtcagt atcgtgcggg ccggcctgcg caaaaggtta cgcaggaaca tacgcgtgat
     2041 agcgggcagg tttttgaagt aacgttcaaa gaacgactgc gcgaaacttt caggaaccgg
     2101 gggccgcatc gcctgcgcag gctgcatacg catcacccgc ttcacggaaa accacgtacc
     2161 gaatacgcca gcgctggcgc cgatgacgag accagacaag gcaaccgaaa gattgaatac
     2221 atgcgtcaaa tcgggaaacc ggtaaaattc gccgtaaagc catgtcatcg agcgtccgag
     2281 gtaccagccg acacccacgc cgagcaaggc acccgagcca ctgatcacaa gcatcagttt
     2341 caggtagtgc actgcaattt cacggttggt atagccgagc gccatcagtg aagcgatctg
     2401 ctcgcgctgg cgggcgatca gccgcgtggc gacgatgtgc agcagaaatg ccgccacacc
     2461 caggaagacg agcggaagga aaaaggcgtt ggt
//
LOCUS       DAOQJF010000433         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_30596_length_2490_cov_4.466530, whole genome shotgun sequence.
ACCESSION   DAOQJF010000433 DAOQJF010000000
VERSION     DAOQJF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30596_length_2490_cov_4.466530"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLF85_16165"
     CDS             complement(<1..513)
                     /locus_tag="PLF85_16165"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNE21255.1"
                     /translation="MRALGVIPARFASTRFPGKPLAKIAGKPMIEWVWGAAKKCSDLS
                     RIVVATDDERIAQAVADFGGEAVMTRDDHVSGFDRIVEVAQKFPDFSHYVNVQGDEPL
                     LPAENISGALALFASHGERCDIATTAVPFAALADFQNENMVKVVLAHDGRALYFSRSP
                     VPFYRKGSFDG"
     gene            complement(513..2345)
                     /locus_tag="PLF85_16170"
     CDS             complement(513..2345)
                     /locus_tag="PLF85_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21256.1"
                     /translation="MPESKYEFDQTRAAIGANELDNAERREMLERFKSAGGQVLREKS
                     VAEKQEEARRAAEARSAGAGGVAARPAGGGGGAIPETKLPSELRREQAREEADRAQRA
                     RAEYEKALKKLKGPGARFMVKLRCFLAGITPFAGNVIKPGFVQFLNLELKQALIEFNL
                     IGNDLFLQRPSIGKKIMHNLDGKNPLLMETLEYAHHMYNSEYFNTFATAAQNGAAVPV
                     ESAAAAIKSIYRTLYILYPFQETFRKAMGYALDVFTPEAQAAKLSQESLQGMILKQKR
                     FHANTKIAFQTAFPKLFHLVCLADGTDYPPFSPLLEKAIQVAQNDKLGQRKKGDGSTL
                     ESQAADQPVEGAQPEEKAEEKKEEEKKGGIYDTKEYQYGFSLMKMRNPQQLVERYDKS
                     KRMLEKVPINDRILLSYLYFMEFDYEYSFVLTTNKISINVDYSGGVKSDYKKQMADLY
                     NESRAIIKAYEKYEEMLQEYRAAKDRKSSNYIEKSKLEEKAKGRADMEGRNTRGLIRA
                     FMDNVAKSMAYLIADMKGAKQIVANMDEAVKFDAEFEKGKRLNGKPVKQCIMDAYCFA
                     VAFKERLANGDLFPLTPMTDEEMQSSFGQSFGVKAAETPDTSEI"
BASE COUNT          494 a          706 c          669 g          621 t
ORIGIN      
        1 cccatcaaaa ctacccttgc ggtagaacgg aaccggcgag cgagaaaaat acagcgcccg
       61 gccgtcatgc gccagcacta ccttgaccat gttctcgttc tggaaatcag ccagcgccgc
      121 aaaagggacc gccgtcgtgg caatatcaca gcgttcaccg tgggatgcaa agagcgccag
      181 cgcacccgaa atgttttctg ccggcagcag gggttcatcg ccctgtacgt taacataatg
      241 cgaaaaatca ggaaatttct gcgcgacctc gacaatgcgg tcaaagcccg aaacatggtc
      301 atcacgggtc atcaccgctt ctccgccaaa atccgccacg gcctgcgcga tacgctcgtc
      361 atcggtagcg acaacaatcc gcgacaggtc gctgcatttt tttgctgcac cccataccca
      421 ttcaatcatc ggcttgccgg caattttagc cagcggctta ccgggaaacc gggtcgaagc
      481 aaaccgggcg ggtataacgc cgagcgcacg catcagattt ctgatgtatc cggggtttca
      541 gccgctttga cgccaaagct ctggccaaag gaggattgca tttcttcgtc ggtcattggc
      601 gtcagcggga acaggtcgcc gttcgccagg cgctctttaa atgccacggc aaaacagtag
      661 gcatccatga tgcactgctt gactggcttg ccgttaagcc gcttgccctt ttcgaactcg
      721 gcatcgaatt tgaccgcttc gtccatgttg gcgacgatct gtttggcgcc tttcatgtcg
      781 gcgatcaggt acgccatcga tttggccacg ttatccatga atgcgcggat caggccacgc
      841 gtgttgcggc cttccatgtc agccctgccc ttcgctttct cttcgagttt ggatttttct
      901 atgtagttcg aggatttccg gtctttggcc gcccggtatt cctgcagcat ttcttcgtat
      961 ttttcgtagg ccttgatgat tgcccgggat tcattgtaca ggtcggccat ctgcttcttg
     1021 taatccgatt tcactccccc ggaatagtcg acgttgatcg agatcttgtt cgtcgtcagg
     1081 acaaacgaat actcgtagtc gaattccatg aagtacaggt aggaaagcag gatacgatca
     1141 ttgatcggca ccttttccag catacgcttg gacttgtcat agcgctcgac cagctgctgc
     1201 ggattgcgca ttttcatgag cgaaaagccg tactggtatt ctttggtatc gtagataccg
     1261 cccttctttt cctcttcttt cttttcctcg gctttctctt cgggctgtgc accctcgaca
     1321 ggctggtcag ccgcctgtga ctctagcgtc gaaccgtcgc ctttcttgcg ttggcctaat
     1381 ttatcattct gcgctacctg gatcgccttt tcgagcagcg gcgaaaacgg cggataatcg
     1441 gtgccatcgg ccaggcatac caggtggaag agcttgggaa acgctgtctg gaatgcaatc
     1501 ttcgtattcg cgtgaaaacg cttctgtttg agaatcatgc cctgcagcga ttcctgcgaa
     1561 agtttggccg cctgcgcctc gggggtaaag acatcgagcg cgtagcccat cgccttgcga
     1621 aaggtctcct ggaagggata gagtatgtac agtgtccggt agatcgactt gatcgccgcc
     1681 gccgcgctct cgacgggtac tgccgcacca ttctgcgcgg cagtcgcgaa ggtattgaag
     1741 tattcgctgt tgtacatgtg gtgcgcgtat tcgagggttt ccatcagcag cgggttcttg
     1801 ccatcaaggt tgtgcatgat ctttttaccg atcgagggtc gctgcagaaa gaggtcgtta
     1861 ccgatcaggt tgaactcgat cagcgcctgc ttgagctcga ggttgagaaa ttgcacaaaa
     1921 cccggtttga tgacattgcc ggcaaagggg gtaatacctg ccagaaagca gcgcagctta
     1981 accatgaagc gtgcaccggg ccccttgagt ttcttgagcg ccttttcgta ctcggcgcgg
     2041 gcccgttgcg cgcgatctgc ttcttcgcgt gcctgttcgc ggcgcaattc tgaaggaagt
     2101 ttcgtctcgg gtatcgcacc tccgccgcca cctgctggcc gggcggcaac accaccggcc
     2161 ccggcagaac gcgcttcagc agcacggcgt gcctcttcct gtttttcagc aacactcttc
     2221 tcgcgcagca cctgcccacc ggcagacttg aagcgttcaa gcatctctct tcgttcggcg
     2281 ttgtcgagtt cgttggcacc tatcgcggcg cgggtttggt cgaactcgta cttactctcg
     2341 ggcattgttt tcctgcagtg cgtcagagac ggtcggtcaa gcaaaacagc aaaacatttg
     2401 ccaacctgtg tcgaatccgg gttcgggata tatgaaagca ggtatacatc ccgtttatga
     2461 agacgcggtg attcgctgcg cgtgcggtgc
//
LOCUS       DAOQJF010000434         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_30756_length_2483_cov_6.541186, whole genome shotgun sequence.
ACCESSION   DAOQJF010000434 DAOQJF010000000
VERSION     DAOQJF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30756_length_2483_cov_6.541186"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLF85_16175"
     CDS             complement(<1..952)
                     /locus_tag="PLF85_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21257.1"
                     /translation="MPELYPNYFFIGSLLTFLFLVYVALLLMRIPERSSASTQLAIAL
                     IFTSIFNFSYVVTHGIYTLPTFYTRWLNFYSAMCAALHVAAFFHSFPRVRSAAAVRYT
                     LIIGHASILLVTGYLAYAMANAPVYYLFNSHFWDSDTLPAQKAVSVLILLLFLHLVAI
                     GIWRGLIEKGEQRIAVWLIVAGFLLATIPPGILQVLSRDNLVPRSSYMTATTILNLLG
                     YFFSVVVYINVTHDRTSVLARITGITLLAVLLIFQAVAYFWMNDAETSYDAVHYGQAR
                     EGYVLGQLPQRAASQVVYDHANGALLSEPDAAYESKQNLLQ"
     gene            1103..1504
                     /locus_tag="PLF85_16180"
     CDS             1103..1504
                     /locus_tag="PLF85_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNE21258.1"
                     /translation="MPQHSVPASVWPAIIQLLFGAGFAGLILLLAHKITPRMHKKAEA
                     HPDTFECGIPYADDARGVFNVKFYLIAMVFIVFDVEAIFLMPWAVSFRSFKDAGMGLV
                     MLIELTVFLAILFVGYYYVIKKGALTWEDPN"
     gene            1504..2106
                     /locus_tag="PLF85_16185"
     CDS             1504..2106
                     /locus_tag="PLF85_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNE21259.1"
                     /translation="MAEKIRNRFTNPETSAQPSGAGPQKGQFDQSFIVGAIDDFVTWG
                     QSYSLWPFPFATACCGIEYMATSASDFDIARFGAERPSFSPRQADMILVLGTITYKMA
                     PVLRNIYEQLAEPRYVVSVGACASSGGMFHTYAVLQGIDRILPVDVYVPGCPPRPEAI
                     LEAVMQLQKKLRSQSIEERREEIRQKLKLIEEKNRPIIVE"
     gene            2127..2483
                     /locus_tag="PLF85_16190"
     CDS             2127..2483
                     /locus_tag="PLF85_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21260.1"
                     /translation="MSQPYYDIQDSRRIFGSEVELMKFLKTLLIKDYKIYEMQNEQGT
                     MVPYHAHAFAETILVLEGMVRMIIEEDIVDLYPGQMVTIQPFAIHLAAFPNEGGARFY
                     LLFAEKGKENIFPKKD"
BASE COUNT          626 a          641 c          661 g          555 t
ORIGIN      
        1 gctgcagcag attttgtttc gattcatatg cggcatcagg ctcggaaagc agcgcaccat
       61 ttgcatgatc gtaaacaacc tgcgacgcag cgcgctgcgg caattgcccg agcacataac
      121 cttcgcgggc ctggccataa tgcacggcgt cgtagctcgt ttcggcatcg ttcatccaga
      181 aatacgctac tgcctggaag atcagcagga cggcgagcag cgtaataccg gtgatgcggg
      241 cgagtacaga agttcggtcg tgggtcacat tgatatacac aacgacagag aagaaataac
      301 ccagaagatt cagaatggtt gttgctgtca tgtagcttga gcgtggaaca agattgtcgc
      361 ggctcagaac ctggagtata ccgggaggaa tcgtcgccag caggaaccct gcgacgatca
      421 gccagacggc aatgcgctgc tcccccttct cgatgagacc acgccatata ccgatcgcaa
      481 cgaggtgcag aaacaacaag aggatcaaaa cactcacagc cttttgggcg ggtagagtat
      541 cagaatccca aaaatgactg ttgaacaggt aatagaccgg tgcgttcgcc attgcatagg
      601 caagatagcc agtcacaagc agaatggaag cgtgcccgat aatgagagta tacctgacgg
      661 ccgcggccga tcttacacgg ggaaacgaat gaaaaaatgc cgccacgtgc agggccgcgc
      721 acattgccga ataaaaattc agccagcgtg tatagaaggt cggcagggta tagatgccat
      781 gtgtgacaac gtaactgaag ttgaagatac tggtgaaaat cagggcaatc gccagttgcg
      841 tgctggcgga tgatctttcc ggaatgcgca tgagaagcag cgccacgtat acaagaaaaa
      901 ggaatgtgag taatgagcca atgaagaaat aattggggta caactcaggc attcccacgt
      961 atgcccggcc taatgtccat gtaaagtaaa gattgtgtac agggtgtttg ccagcgaggg
     1021 gggacaaacc ggaaaagccg gtagtgggta ccccagagtg ttttttactt cacggcttca
     1081 ccgactttgc gtttgctgct gcatgcctca gcattcggtt cctgcctcgg tatggcctgc
     1141 catcatacaa ctgcttttcg gcgccggttt tgcgggtctt atcctgctgc ttgcgcacaa
     1201 aatcaccccg cgcatgcaca agaaagccga agcgcaccct gacacctttg agtgcggtat
     1261 accctacgcc gatgacgcgc gcggcgtatt caacgttaaa ttttacctca tagcaatggt
     1321 atttatcgtt tttgacgtcg aggcaatctt cctgatgccc tgggcggttt cgttccgttc
     1381 gttcaaagat gccggcatgg ggctggtaat gctcatagaa ctcaccgtgt ttttggccat
     1441 actttttgtg ggatattatt atgttattaa gaaaggtgcc ctgacttggg aggaccccaa
     1501 ttgatcgctg aaaaaatccg taatcgtttt actaaccccg aaacgtccgc gcaaccgtca
     1561 ggcgcggggc cgcagaaagg ccagtttgac cagagtttca tcgtcggtgc aatcgatgac
     1621 tttgtcacct ggggccaatc ttactcgctt tggccttttc cttttgctac cgcctgctgc
     1681 ggtattgaat acatggcaac gagtgcctct gactttgaca ttgcgcgttt cggcgcagaa
     1741 cggccttcct tttcaccccg ccaggcagac atgattctcg tgctgggcac gatcacctac
     1801 aagatggcgc ctgttttgcg caacatctac gagcagctgg ccgagccacg ctacgtggtc
     1861 tcggttgggg cctgcgcctc ttcaggcggc atgttccata cctacgcggt tttgcagggt
     1921 atcgaccgca ttctgcccgt cgacgtctat gtacccggct gcccgccacg ccccgaggcg
     1981 attctcgaag ccgtgatgca gctgcagaag aaactgcggt cgcagagtat cgaagagcgg
     2041 cgcgaagaaa ttcgccagaa gctgaagtta attgaagaaa aaaatcgtcc aattatcgta
     2101 gaataagcaa aaggagctca agcgttatga gtcagcccta ctacgacatc caggattcgc
     2161 gccgcatctt cggcagcgaa gtggagctca tgaaatttct caagacgctg ctcatcaaag
     2221 actataagat ctacgaaatg cagaatgagc agggcaccat ggtaccttac catgcgcacg
     2281 cgtttgctga aactatcctc gtgcttgagg gcatggtacg gatgatcatc gaagaagata
     2341 tcgtcgacct gtaccctggg cagatggtca ccatccagcc atttgcgatt cacctggccg
     2401 cttttcccaa cgaaggcggc gcgcgcttct acctgctctt tgccgaaaag ggcaaagaaa
     2461 acatcttccc gaagaaagac taa
//
LOCUS       DAOQJF010000435         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_30763_length_2483_cov_3.704695, whole genome shotgun sequence.
ACCESSION   DAOQJF010000435 DAOQJF010000000
VERSION     DAOQJF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30763_length_2483_cov_3.704695"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..754
                     /locus_tag="PLF85_16195"
     CDS             236..754
                     /locus_tag="PLF85_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21261.1"
                     /translation="MAFSADGNTMVAAMFGGKTDADQAGRTALIDLNTQKITSTIISG
                     GAHRHAVRLRTGADVFAVSDMAKNRVYFIENYKKVDETKVFSNPNTIVESPDGKYLYV
                     SCRGHNNPKSFLIKGPDFGKVMVIDLATRKVVEEIEGGNQPTGLDVSPDGKYIVSTDF
                     LDHAMRVYERIP"
     gene            complement(831..1313)
                     /locus_tag="PLF85_16200"
     CDS             complement(831..1313)
                     /locus_tag="PLF85_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21262.1"
                     /translation="MEYMCYSNNMLGLDATGQMIAANTAGTQVFRFNPNTVASQCSVA
                     NVANPKLSVGTHISLMRQASTGIADGYVVSDTAGKVSAQSVTSSGPQLQGLIDGLARN
                     GLISTGVSVMQGASEAGDLITFSAAPSVGGLSDLYVLLSPKPANLWQQYVAAGSQPSP
                     "
     gene            complement(1886..>2483)
                     /locus_tag="PLF85_16205"
     CDS             complement(1886..>2483)
                     /locus_tag="PLF85_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNE21263.1"
                     /translation="ELEQKVQQELKALGMEETQIKVSIRWEYADDGTVTQDSSPDKKY
                     VIAPTGLDIVEFLMASSSRETLRPLRKIASGGEMSRIMLALKKVIIETDPVYTMVFDE
                     VDAGVGGRIAEAVGRKLAGLSLSSQVIVITHLHQIAGLSPNEVRHFKVSKHKEDGTRI
                     TRLSKEQRLQEIARMIAGENITESALSHAKALLAEKIQ"
BASE COUNT          637 a          647 c          658 g          541 t
ORIGIN      
        1 aataaggtag tcaatagaca actactccat cgactattcc ttctataaaa taaagaaaca
       61 caatactaga aagaaatgca acaaagtaga aataataaaa aacaaataag tatagcaaat
      121 agtaataaag aagaaataac atataaaaag gaaatagcta ataaaatgag aattaattaa
      181 agataaaaag attaaagaaa taaagaagat aaaactgggc agcgtgccac gcggcatggc
      241 gttcagcgcc gacggcaata ccatggtggc ggcgatgttt ggcggtaaaa ccgacgccga
      301 ccaggcaggg cgaaccgcac tcatcgacct caacacacag aagatcacca gcacaatcat
      361 ctcgggcggc gcacaccgcc acgcggttcg cctcagaact ggcgcggatg ttttcgccgt
      421 atcggatatg gcgaagaacc gggtgtactt catcgagaac tacaagaaag tcgacgagac
      481 gaaagttttt tcgaacccca acacgatcgt cgaatcaccc gatggcaagt atctgtacgt
      541 ctcgtgccgc gggcataaca acccgaaatc atttctcatc aagggccctg acttcggcaa
      601 ggtcatggtg atcgacctgg caacacgcaa ggtagtcgaa gagattgaag gcggcaacca
      661 gcccaccggg ctcgacgtgt cacccgacgg taagtacatc gtttctaccg attttctcga
      721 ccacgcgatg cgcgtgtacg agcggatacc ttaaccaccg cagtcactca gcagggtccg
      781 gaaaaactcc cacaccggaa cctgcttcag gctccttttt caggagcctg ctagggtgat
      841 ggctgcgagc ccgcagcgac gtactgttgc cagaggttcg cgggtttcgg cgatagcagt
      901 acataaagat cagataaacc acccaccgaa ggcgcggcag agaaagtgat caggtcaccc
      961 gcttcgcttg caccctgcat cacgctgacg ccggtcgaaa tgagaccatt gcgcgccagg
     1021 ccatcgatca agccctgcag ctgcggcccc gaactcgtca ccgactgcgc gctcaccttg
     1081 cccgcggtgt cgctgaccac gtaaccgtcc gcaatccccg tgctggcctg ccgcatcagg
     1141 ctaatgtgtg taccaaccga tagcttgggg ttagcgacgt ttgcaacaga gcattgactg
     1201 gccacggtat tcgggttgaa gcgaaagacc tgcgtacccg cagtattggc ggcaatcatc
     1261 tgccccgtcg catcgagacc gagcatgttg ttggaatagc acatgtattc cataatcagg
     1321 ttcgggccgg tggataagtt tatcggactg gcggagaccg gattaaaagg ttcggaagtc
     1381 ataatttgac caccacttgt ctgcagataa accgtcacat tcgtaccact agctacagca
     1441 gccaaccgcg ggcgcgaact atagtttaga ggactgaaac cattcatgac gaacggctgc
     1501 ggagtgttga aggcaggcaa cgtacccgtg cctgcatagg cagaaaccga cgaagcgaca
     1561 aaaatgttat gattggtgca tgaaagcgcg cagctacggg tagacccaaa aaccagtgcc
     1621 aggtagttgt tggacaacct gacaattatc gggctgtaat cgtcggtggc agactgcgaa
     1681 atgctgctgc cgtccgcgag tttcagtctg ctcggttcga ttccttcgaa ggtgacttca
     1741 gagtttgagc aggcaaccgt attcaaggcg accgcagcgg gaatggttag caataatttg
     1801 cgtaggtatt tcgggctcat agatatatgt agcccggtat gccgcggtcg tcaagaataa
     1861 ttgctaattt tgtcttttat ttgttttact gtatcttctc tgccaagagc gccttcgcgt
     1921 ggctgagcgc cgattcggtg atgttctctc ccgcaatcat gcgcgcgatt tcttgcaggc
     1981 gctgttcttt cgaaagccgt gtgatgcgcg tgccgtcttc tttgtgtttg gagaccttga
     2041 agtggcgcac ctcgttgggt gagagacccg caatctggtg caggtgcgtg atcacgatca
     2101 cctgcgaaga gagcgaaagc cccgcgagct tgcggcccac ggcttcggca atgcggccgc
     2161 cgacacccgc gtcgacctcg tcgaacacca tcgtgtagac cgggtcggtt tcaataatga
     2221 cttttttgag cgcgagcatg atgcggctca tttcaccgcc agaggcgatt ttgcgcagcg
     2281 ggcgtagtgt ctcgcgcgat gacgacgcca tcaaaaattc gacgatgtcg agaccagtgg
     2341 gtgcgatcac gtattttttg tcaggcgatg aatcctgtgt gacagtaccg tcatcagcat
     2401 attcccagcg gatagagacc ttgatctgcg tctcttccat accgagcgct ttcagttctt
     2461 gttgcacttt ctgttcgagt tcg
//
LOCUS       DAOQJF010000436         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_30951_length_2475_cov_4.506198, whole genome shotgun sequence.
ACCESSION   DAOQJF010000436 DAOQJF010000000
VERSION     DAOQJF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30951_length_2475_cov_4.506198"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PLF85_16210"
     CDS             complement(<1..142)
                     /locus_tag="PLF85_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE21264.1"
                     /translation="MRGMLSSQITLHNGQKIPQLGFGVWKAAEGECYKAVRTALEVGY
                     RHI"
     gene            258..1436
                     /locus_tag="PLF85_16215"
     CDS             258..1436
                     /locus_tag="PLF85_16215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE21265.1"
                     /translation="MDGASFSIFSFALSVLLNFLANYVAWRSTGIAALHIWAISNLIT
                     FAGFGALFFYKQSGWKWLILLQNAFYLISFILLPAGILQYRGLRFRLSGHLAILTVTL
                     AAISWGLWVRESFVFRTFVGTLAFAGYCFYIVYLILRHTRGQSALVKFAIGTWGAYGV
                     IHLIRLSMIAMGMGIDDGQPFQGIAYLLAFVFGPVCTTGGYIGLILLIVQRLVDEKNS
                     SLAAAERLALQYRELSDHDPLTNALNSRSFMAELSRLLEKSRSAQAPLCVLMADLDHF
                     KRINDTYGHAAGDITLKHAVQVWQGLLRPADCLGRVGGEEFAIILPNTDRSEAAHVAE
                     RLRQGIASTKMEAPENITASFGAAQMVPGETAEDLLRRADNAMYEAKNTGRNRVVTAK
                     "
     gene            1479..>2475
                     /gene="leuB"
                     /locus_tag="PLF85_16220"
     CDS             1479..>2475
                     /gene="leuB"
                     /locus_tag="PLF85_16220"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNE21266.1"
                     /translation="MQYDIAVLAGDYIGPEVMKSALEVLGKVADKDAQFNFKEALVGG
                     AAIDATGKALPPETLKTAESAHAILFGSVGGPKWEHMPPAEQPERASLLPLRKHFALY
                     ANLRPAIIYKELKEASPLRADIVGDGLDVLILRELTGDVYFGEPKIRRGEGANEEALD
                     TMIYKRFEIERIAHLAFQSARLRRKVVHSIDKANVLTSMVFWREVVTEVGKQYPDVKL
                     SHMYVDNASMQLIRWPNQFDVVLCPNMFGDILSDEASQITGSIGMLASASLGEAGKWG
                     RDGLQFGLYEPAGGTAPDIAGQGVANPIAQILSAALLLRYSLKLEDKAVKIENAVK"
BASE COUNT          526 a          694 c          678 g          577 t
ORIGIN      
        1 cgatatgacg gtagccgact tcgagcgctg tgcgcacggc tttgtaacat tcgccttcgg
       61 cggccttcca cacgccaaaa ccaagttgtg ggatcttttg gccattgtgg agggtgattt
      121 gtgaggagag catacccctc attttgttcg ccgatacagt gcgtctagtg ataagaagtt
      181 tactgacaag tcactaatgg tatggaattc tctatttaca ccggtgggta taattatgat
      241 tgatcgcgtc tactcacatg gatggcgcct ccttttccat ctttagtttt gccctgagcg
      301 tcttgctgaa ttttctggca aactacgtcg cctggcgttc gacgggtatt gcggcattgc
      361 atatctgggc tatttcaaat ctaatcactt ttgcaggctt tggtgcgctt ttcttttata
      421 aacagtccgg ctggaaatgg ctcatcctat tacagaatgc attctacctt atctccttta
      481 tcttgttgcc agccggcata ctgcagtaca ggggcctgag gtttcgcctg tctggacatc
      541 tggcgatttt gactgtaacg ctggcggcaa tcagctgggg tctctgggta cgggaaagtt
      601 ttgtctttcg aacttttgtt ggcacgctag catttgcggg ctattgcttc tatatcgtgt
      661 acctgatttt acgacacact cgcggccaga gcgcgctggt gaagtttgcc atcggcacct
      721 ggggcgccta tggtgtgatc catctcattc gactgagcat gattgccatg ggaatgggta
      781 tcgacgacgg acagcccttt cagggtatag cgtacctgct cgcttttgtt ttcggccccg
      841 tctgcacaac cggcggttac attgggctca ttctgctcat tgtacaacgc ctcgtcgacg
      901 aaaagaactc ctcactcgct gcagctgaac gcctcgcgct gcaataccgc gaactctctg
      961 atcacgatcc gctcaccaat gctctcaata gccgtagctt tatggcggag ctttcccgtt
     1021 tgctggaaaa gagccgtagc gcacaagctc ccctctgcgt gctgatggct gaccttgacc
     1081 atttcaaacg catcaacgat acgtacggtc acgcagcagg tgacataaca ctcaaacatg
     1141 cagtacaggt ctggcaaggt ctgttgcgac ccgccgactg ccttggccgc gtggggggtg
     1201 aagaattcgc catcatatta ccaaatacag atcggtctga ggccgcgcat gtggccgaaa
     1261 ggctgcggca gggcatcgcc tccaccaaga tggaagcgcc agagaatatc acggcgagct
     1321 tcggagcggc gcaaatggtc cccggtgaga ccgccgaaga tttactgcgg cgcgctgaca
     1381 acgccatgta cgaggcaaag aataccggcc gtaaccgggt ggttacggct aaatagccat
     1441 tcgatttaca gcctgtctgc acgacaggtt agtcctctat gcaatacgat atcgcagtgc
     1501 tcgcgggcga ctacatcggc cccgaggtca tgaaatcggc cctcgaggtg ctcggcaaag
     1561 tcgccgacaa ggacgcccag ttcaatttta aagaagccct ggtcggcggc gcggccatcg
     1621 acgccaccgg taaggcgctg ccacccgaaa ctctcaagac cgccgaatcg gcgcacgcga
     1681 tcctgtttgg ctcggtcgga ggtcccaagt gggagcatat gcctcctgct gagcagcccg
     1741 agcgtgcatc gcttctaccg ctgcgtaaac acttcgcgct ctacgcgaac ctgcgcccgg
     1801 cgatcatcta caaagaactg aaagaagcgt cgccgctgcg tgctgacatc gtcggtgacg
     1861 gcctcgacgt gctgattctg cgtgaactca ctggggatgt ctacttcggt gaacccaaga
     1921 ttcgccgggg tgaaggcgct aacgaagaag ccctcgacac gatgatctac aagagatttg
     1981 aaatcgagcg tatcgcgcac ctcgcctttc aatccgcccg gctgcgccgc aaggtagtgc
     2041 actcgatcga taaggcgaac gtgctcacca gcatggtgtt ctggcgcgaa gtcgtcactg
     2101 aagtcggcaa gcagtacccc gatgtgaaac tcagccacat gtacgtcgac aacgcgtcga
     2161 tgcagctgat ccgttggccg aatcaattcg acgttgtgct ttgcccgaac atgttcggcg
     2221 acatcttgtc tgacgaagcg agccagatca ccggctcgat cggcatgcta gcctcagctt
     2281 cgctcggcga agccggcaag tggggccgtg acggcctgca gtttggtctc tacgaacccg
     2341 ccggtggcac agctcccgac atcgcgggcc agggcgtcgc gaacccgatc gcgcagattc
     2401 tttcggcagc gctgctgctc aggtactcac tcaaactcga agacaaggca gtgaagattg
     2461 agaatgcggt gaaat
//
LOCUS       DAOQJF010000437         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_31131_length_2465_cov_4.102905, whole genome shotgun sequence.
ACCESSION   DAOQJF010000437 DAOQJF010000000
VERSION     DAOQJF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31131_length_2465_cov_4.102905"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLF85_16225"
     CDS             <1..286
                     /locus_tag="PLF85_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21267.1"
                     /translation="DDPKICFNTCEEYCAEGIPTDEPITNCGQFCPVKGKVPNACPKN
                     MTREQYKAKFSRKYEIVLQLNKKGEVVVKKAPKGQNSFSVGQNFGRLYEK"
     gene            complement(334..1170)
                     /locus_tag="PLF85_16230"
     CDS             complement(334..1170)
                     /locus_tag="PLF85_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE21268.1"
                     /translation="MGDARKIQQVLKGQETSDGAGVKLRRYIGTRDLMAHDPFLMLDA
                     FFSDDPDSYLAGFPNHPHRGFETVTYLIQGKMEHNDSRGNKGLLEPGGAQFMRAGSGI
                     IHSEMPRQENGLMWGYQLWVNLPAALKMTEPHYQDMQAHEIPVVETESYTAKVLSGSL
                     GGVQGGARNFYPIDYFDVQFKPGQSAEIPVAADRSAFVIVVAGSGEISGQKISRGDLP
                     ILGSGDVIQFKADEELRVIIVAGKPIKEPVARYGPFVMNTPQEIEQAFVDYQAGKFSR
                     AS"
     gene            complement(1163..1687)
                     /locus_tag="PLF85_16235"
     CDS             complement(1163..1687)
                     /locus_tag="PLF85_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21269.1"
                     /translation="MNLIYLHLALVAALLGIAAGASDRNPEQFTPNQANWKTFESKPF
                     ADSRNPTRAEVDEWHRIAINHVRALVGYTGADSEVKKDHCMFKRALRCHLPARCERPV
                     ALPAGRPCDVQSAAGCRGSFQRIEIEHTLVDQMVALAVRQVDRQADAESVQETVKFGI
                     FCRSCTFALHCVYG"
     gene            1884..>2465
                     /locus_tag="PLF85_16240"
     CDS             1884..>2465
                     /locus_tag="PLF85_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNE21270.1"
                     /translation="MQKPTLLILAAGMGSRYGSLKQMDGLGPGGETIMDYSIYDAIRA
                     GFGKVVFVIRKSFADDFKAVFSAQRYQGKIAVEYVMQELDMIPSEFKLNPERTKPWGT
                     NHAVMVASGVIKEPFAVINADDYYGRNAFEVLANFLTGVTGKQNDYCMVGYQLGKTLS
                     ELGAVSRGVCSADTNGYLTTVVERTHIERIEGTI"
BASE COUNT          557 a          664 c          685 g          559 t
ORIGIN      
        1 tgatgaccca aaaatctgct tcaatacatg cgaagaatac tgcgccgaag gtatacctac
       61 cgatgaaccg atcacgaact gcgggcagtt ttgccctgtg aaaggcaagg tgccgaacgc
      121 ctgcccaaaa aacatgaccc gcgaacagta caaggccaaa ttcagcagga agtatgagat
      181 cgtgctgcag ctcaacaaaa aaggcgaggt agtcgtaaaa aaagcgccga aaggccagaa
      241 tagctttagc gttggccaga actttggccg cctctatgag aagtaggcgg agcgactacc
      301 gcgtcgacca gaagtttctt ctttagatag atgctacgat gcgcgcgaaa atttgcctgc
      361 ctggtaatcg acaaaggcct gctcgatttc ctgcggagtg ttcatgacaa acggaccgta
      421 gcgcgctaca ggttctttga taggtttccc cgcgacgatg atcacacgca gttcttcgtc
      481 ggctttgaac tggattacat cgcctgaacc cagaattggc aggtcaccac ggctgatctt
      541 ttggcctgaa atttcgccag agcctgcgac cacgatgaca aaagcagaac gatcggccgc
      601 aacgggaatc tcggcgctct gtcccggctt aaactgcacg tcaaaataat cgattgggta
      661 gaaattacgc gcaccgccct gaactccgcc taagctgccc gacagcacct tcgctgtata
      721 actttcagtt tcaacgacgg ggatttcatg cgcctgcata tcctggtagt gcggttctgt
      781 catcttgagc gcagcgggta agtttaccca cagctggtag ccccacatga ggccgttttc
      841 ctgccgcggc atttcagagt ggataatacc cgagcccgcg cgcatgaact gcgccccgcc
      901 cggttcgagc aggcccttgt taccgcgcga gtcattgtgc tccatcttac cctggatcag
      961 gtatgtcact gtttcgaagc cgcggtgcgg gtggttggga aaacccgcaa ggtatgaatc
     1021 ggggtcgtcg ctgaagaagg catcgagcat caagaacggg tcatgcgcca tgaggtcacg
     1081 cgtgccgatg tagcggcgca atttcactcc tgcgccgtcg gatgtttcct ggcccttcag
     1141 tacctgttga atttttcgcg catcacccat acacacagtg tagtgcgaat gtgcacgaac
     1201 gacaaaatat tccgaacttt acggtttctt gtacagattc ggcatcagct tgccggtcca
     1261 cttggcgcac agcgagcgcg accatttgat cgaccagggt atgttcgatt tcgatgcgct
     1321 gaaaactccc tcggcaccct gctgcgcttt gcacgtcgca tggcctgccg gcaggtaagg
     1381 cgactggtcg ctcgcatcgg gcaggaaggt ggcaccgcag tgcgcgcttg aacatgcagt
     1441 ggtccttttt cacctcgctg tcggcgccgg tataaccgac gagcgcgcgc acgtggttga
     1501 tcgcaatgcg gtgccactcg tcgacttcgg cacgcgtggg gtttctgctg tcggcaaaag
     1561 gtttcgattc aaacgttttc cagttagcct ggttgggggt gaactgctcg gggttacggt
     1621 cgctggcgcc ggcagcgatg ccaagcagtg cggcgacaag agcaaggtgg aggtatatga
     1681 gattcatgta tggcttcctg gttgattcta tgaacgaatc tgcatgaacc cttacatgag
     1741 ggtggggtca aatcatttgt ttcttggcta gtgacagtta ttgcgccagc ggcaacatag
     1801 ttgatgcctg cgcaaggaga cagatgaaga tcctctaccc gataatgtca ctgacagccg
     1861 atttctgaaa tcggttctgg cgcatgcaaa aacccaccct actcattctc gcggccggta
     1921 tgggcagccg ctacggcagc ctcaagcaga tggacggtct ggggccgggc ggtgaaacca
     1981 tcatggatta ttccatttac gacgccattc gggcgggttt cggtaaggtc gtctttgtca
     2041 ttcgcaaaag cttcgccgat gacttcaagg cagtcttctc agcgcagcgt taccagggca
     2101 agatcgcggt tgaatacgtg atgcaggaac tcgacatgat tccctctgag ttcaagctca
     2161 accctgagcg caccaagccg tggggtacaa atcatgctgt catggtcgca tcgggagtga
     2221 tcaaagagcc ctttgccgtc atcaacgccg atgactacta tggtcgcaat gcctttgaag
     2281 tcctcgcaaa ttttttgacc ggcgtcacgg gtaagcagaa cgactactgc atggtcggtt
     2341 accagctggg taagacactg tcagagctcg gtgctgtctc gcgcggcgtc tgctcggccg
     2401 atacgaatgg ttacctgacc accgtggtcg aacgcacgca tatcgagcgc atcgaaggca
     2461 ctatt
//
LOCUS       DAOQJF010000438         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_31545_length_2446_cov_4.308239, whole genome shotgun sequence.
ACCESSION   DAOQJF010000438 DAOQJF010000000
VERSION     DAOQJF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31545_length_2446_cov_4.308239"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /gene="dnaN"
                     /locus_tag="PLF85_16245"
     CDS             <1..1133
                     /gene="dnaN"
                     /locus_tag="PLF85_16245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNE21271.1"
                     /translation="THKDSIDKAVRRIDAIIPHRDMGTMLSNLLVAVEAKQVSITASD
                     METTARITVPVESGQAGEFIIPAKQFQEYVGSLVSKDLIVEVETEENPDEGAAAFRVN
                     LRGTSDASARYVMPGNSAKHFPTLNRLSENKLFSVSSALLEEMIRKTQYAISQEDNRY
                     IYNGVCFQGNGDQLTLVGTDGRRLAAITRKLGGPVNLPQGADVVVHAKAIREMQKLID
                     VADEVLIGVEQRDLFVRVGDAQLSSRLLEGKFPDYKRVLPQEIPVQLELSRDKLIEAL
                     QQIRPVTEPPSHQCRMILESKKMHITGQSSGQGSRADVAIDADYDGERLEIGFNITYL
                     LDILKALTCPRVRLGMNDANKPLVVQDVDDPDFIALVMPMKI"
     gene            1156..2322
                     /gene="recF"
                     /locus_tag="PLF85_16250"
     CDS             1156..2322
                     /gene="recF"
                     /locus_tag="PLF85_16250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNE21272.1"
                     /translation="MTEAQNIQPVTIHGLTLANFRNAESAQYTFGTGLIFITGNNGAG
                     KTTILEAIGLTSFLRSFRFALDREMIRFGSSFYRIETGFSVGTAKHRLSIAFGRNPED
                     PKAALVKKYMFDGRANTRAAEIIGRIPTVVLSPDDLRIIAGDHAERRRFLDLLLSMLY
                     PAYFAALQQYQRALKMRSQALKSRPDEGMLAAIDRELASAGVQILEKRRQFIPEFTAP
                     FNENVARISAGKDAWKIDYLGDTRGVNTLEDYMAMLRDRRANDLRLRQTTAGIHRDRI
                     FFLSDGSLRELQAAGDATALPDIRTVGSQGQKRTAALALKMAQFNYMQAKLGLRPVLL
                     IDDVLNELDLGRRASFVDFLGNVGQVFFTTTDLVGMQDFLKNLHQTTPVQNIEL"
BASE COUNT          564 a          726 c          664 g          492 t
ORIGIN      
        1 agacgcacaa agactccatc gacaaggcgg tgcggcgcat cgacgccatt ataccccacc
       61 gcgacatggg tacgatgctc tcgaacctgc tcgttgctgt tgaggccaag caggtctcga
      121 tcactgcgtc agacatggaa acaacggcac gcattaccgt acccgtcgaa agtggccagg
      181 cgggggagtt cataattcct gcaaaacaat tccaggaata cgtcggctcg ctggtttcta
      241 aagacctgat cgtcgaggtc gaaacagaag aaaatcccga cgaaggtgca gccgccttca
      301 gggtcaacct gcgcggcacc agcgacgcat cggcgcgtta tgtgatgccg ggcaactctg
      361 ccaaacattt cccgacgcta aaccgcctga gcgagaataa acttttcagc gtttcatctg
      421 ccttgctcga agagatgatc cgcaaaacac agtacgcaat ttctcaggaa gacaaccgct
      481 atatctacaa cggtgtctgc ttccagggta acggcgacca gctgacgctc gtcggcaccg
      541 acggccgtcg ccttgccgca atcacccgta agcttggcgg accggtaaac ctgccgcaag
      601 gtgcagacgt ggttgtacac gccaaggcga tacgcgagat gcagaaactg atcgatgtcg
      661 ctgacgaagt actcatcggc gtcgagcagc gtgacctctt cgtgcgcgtg ggcgacgcgc
      721 agctgtcgag ccgcctgctc gaaggcaagt tcccagacta caagcgcgta ctgccacagg
      781 aaattccggt gcagcttgaa ctcagccgcg acaagctgat cgaagcactg caacagattc
      841 ggcccgtgac agaaccgcct tcacaccagt gccgcatgat tcttgagtcc aagaagatgc
      901 acattaccgg gcagtcttcg ggtcagggca gccgcgccga tgttgccatc gacgctgact
      961 acgacggcga acgcctcgaa attggcttca acatcactta cctgctcgac atactcaagg
     1021 cactcacctg cccgcgcgtt cgcctcggca tgaacgatgc aaacaagccg ctggtagtac
     1081 aagatgtcga tgatccggat ttcatcgcgc tcgtgatgcc gatgaagatc tgacgcagat
     1141 tcggctcaag cctaagtgac cgaagcccaa aatatccagc ctgtcactat acacggcctg
     1201 acgctcgcga atttccgtaa cgccgaatct gcccagtaca cttttggcac agggcttatt
     1261 ttcatcacgg gcaacaacgg tgccggcaaa acgacgattc ttgaagccat cggcctgact
     1321 tcgttcttac gctccttccg ctttgcgctc gaccgcgaga tgattcggtt cggcagcagc
     1381 ttctaccgca tcgaaaccgg tttttcggtg gggacggcaa aacaccggct gtcgattgca
     1441 ttcgggcgta accccgaaga ccccaaagca gcgctcgtaa agaaatacat gttcgacggc
     1501 cgggccaaca cgcgtgcggc agaaattatc ggtcgcattc cgactgtcgt cctctcacct
     1561 gacgacctgc gcatcattgc aggcgaccat gcggaacgtc ggcgctttct cgacctgctg
     1621 ctttcgatgc tttacccggc ctatttcgcg gcgctgcagc aataccagcg ggcgctgaag
     1681 atgcgcagcc aggcgctgaa atcgcggcct gacgagggta tgcttgcggc catcgaccgc
     1741 gaattggcca gcgcgggcgt gcagatactc gagaagcgcc gccagtttat acccgaattc
     1801 accgcaccct tcaacgagaa tgtcgccagg atttccgcgg gtaaagacgc ctggaaaatc
     1861 gattacctcg gcgacacgcg cggtgtgaat acgcttgaag actacatggc aatgctgcgc
     1921 gaccgccgtg cgaatgacct gcgcctgcgg cagaccactg ctggcataca ccgcgaccgt
     1981 atattctttc tctctgacgg cagcctgcgc gaattgcagg cagcaggcga tgcaacagcc
     2041 ttaccagaca tccgcaccgt gggttcgcag gggcagaaac gcaccgccgc actcgcgctg
     2101 aaaatggcgc agttcaatta tatgcaggca aaactcgggc tgcggccggt cttgctgatc
     2161 gacgacgtgc tgaacgaact cgacctgggc aggcgcgcca gtttcgttga ctttctgggg
     2221 aatgtcggcc aggttttttt tactacaacc gacctggtag gcatgcagga ttttctaaag
     2281 aacctgcacc agacgacgcc ggtgcagaac atagagttgt aatccagaat gcgggctacg
     2341 cccgcatgct ggattcaaat tacttaaggt tagccgattc tttgatgata tacgcgccga
     2401 tttcgttctt ctgaatctgg ctggtgcctt cgtagatcgt gaggat
//
LOCUS       DAOQJF010000439         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_31684_length_2440_cov_5.271698, whole genome shotgun sequence.
ACCESSION   DAOQJF010000439 DAOQJF010000000
VERSION     DAOQJF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31684_length_2440_cov_5.271698"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="PLF85_16255"
     CDS             complement(<1..1637)
                     /locus_tag="PLF85_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21273.1"
                     /translation="MTKSTKQLLESLSKASLSRKILIAIVTIVLILSAVTLLFIDQGV
                     RRQVDKNILTQLDAAATAYVELNDAKLEQVLARTQFFVQNPVFQRDMRGAPATSARIV
                     TGFKKLTEFDFLMAVNFSGRVLVDSIHPEQLGKTVPAQYQDLLQKTVENGRYLGMWSE
                     NGRLYQLVGLPLLGFDAVLLAGNEVTDKFAYKIDELSRNQITFFIGDKVVASAFAKEL
                     SADLEKSLGAVKERISKDAGEGKSKHTQTFSLNVGSESYVAIAGAMENVPEAGFIIAS
                     SKDKQLALLYRIEFIIIITGIVGLIFAIAMAVFFSRGLTRSVDLLVKDVEQVKQGNLE
                     HKIVSTSQDEIGFLSDMFDSLRIAFRDAQKSLKEYAENLEDKVRERTAELSQANSALT
                     AAKGETDRIMETVGQGLFLVHKDGEQYKLGSQYSKALEDMFASPALGGKDLLAILRTG
                     LPEAAMKKTADYLELMFKPEIKEAVMNNINPLREIEINFDSAGAPVKQKFLQFKFKRI
                     MDGTTIQHLMAAVTDVTKQVLLDRKLKETEEKNAGQM"
     gene            complement(1621..2292)
                     /locus_tag="PLF85_16260"
     CDS             complement(1621..2292)
                     /locus_tag="PLF85_16260"
                     /inference="COORDINATES: protein motif:HMM:NF026037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNE21274.1"
                     /translation="MKFLKAFSTLTLSLLVAASSAHAGGTIEGTVTVKTKGLFGKQGA
                     KGDASNVVIFLEELQMPNPNGREKIVQKGKQFQPRVLPIVMGGVVEFPNEDPFNHNVF
                     SPTPEYKFDLDYYGTGKSKNVRFQKTGAVRIYCNIHSNMVSDVLVVPNPFFAKTGADG
                     KFRINNIPPGKYTFVAWQPSGASEKRGIAIVDGKTLQVNFEVEESVFSIKHKNKYGRA
                     YDKEY"
BASE COUNT          546 a          622 c          623 g          649 t
ORIGIN      
        1 tccatctggc cggcattttt ctcttctgtc tctttcaact tgcgatcgag caagacctgc
       61 ttggtgacgt cggtgactgc cgccatgagg tgctgaatgg tcgtgccatc cataatccgc
      121 ttgaacttga actgcaaaaa cttctgtttc acaggcgcgc cagcgctgtc aaagttgatc
      181 tcgatttcgc gcagcggatt gatgttattc atcaccgctt ctttgatttc gggcttgaac
      241 atcagctcca ggtagtcggc ggtctttttc atcgccgcct caggtaaacc tgtgcgtagt
      301 atagccaaca ggtctttgcc accgagcgcc ggcgaggcaa acatgtcttc cagtgccttg
      361 gaatactggc tacccagctt gtactgctcg ccatctttat gcaccaggaa aagcccctgg
      421 cccactgttt ccatgatgcg gtcggtttca cccttcgctg cggtcagcgc gctgttcgcc
      481 tgcgataact ctgcagtgcg ttcgcgcacc ttgtcttcga ggttttctgc gtattctttc
      541 aggcttttct gagcgtcgcg gaatgcgata cgcagagaat cgaacatgtc agataagaag
      601 ccgatctcat cctgcgatgt ggatacaatc ttatgctcga ggttaccctg ttttacctgc
      661 tcgacatctt tcacgagtaa gtcgaccgat cttgtcaagc cgcgcgagaa gaagactgcc
      721 atcgcgatgg caaagatgag accgactatg ccggtaatga tgatgataaa ttcgatgcgg
      781 tagagcaaag caagctgttt gtctttcgac gaggcgataa taaatcccgc ctccggtaca
      841 ttttccatcg caccggcgat tgcgacatac gactcactgc caacgttgag cgagaaggtc
      901 tgcgtgtgtt tgcttttacc ctcgcctgca tctttgctga tacgctcttt gaccgcgccg
      961 agcgattttt cgagatcagc cgagagctcc tttgcgaacg ccgaggccac gaccttatca
     1021 ccgatgaaga aggtaatctg gttacggctt aattcatcga tcttgtaggc aaacttgtcg
     1081 gtgacttcgt tacccgcgag aagaaccgca tcaaaaccga gcagaggcag ccccacgagc
     1141 tggtataacc tgccgttttc gctccacatt cccaaatagc gaccattttc gactgttttc
     1201 tgcagcaagt cctggtattg ggcgggcacg gtcttgccca gctgctcagg atgtatactg
     1261 tcgaccagta cgcggcccga gaaattcacc gccatgagaa aatcaaactc ggtgagtttt
     1321 ttaaacccgg ttacgatacg cgccgatgta gcgggtgcac cgcgcatatc gcgctgaaaa
     1381 acaggattct ggacgaaaaa ctgagtacgc gccagaacct gctcgagctt ggcgtcgttt
     1441 agttcaacgt aagcagtcgc tgcagcatcg agctgcgtca ggatattttt gtctacctga
     1501 cggcgcacac cctggtcgat gaagagcagg gtgactgcag aaagaatcag aacgatcgta
     1561 acgatcgcaa tgagtatctt tcggctgagg ctggcttttg agagactttc gagaagttgc
     1621 ttagtactct ttgtcatagg cacggccgta cttattcttg tgcttgatgc taaaaacgct
     1681 ctcttctact tcaaagttta cctggagcgt cttaccatcg acaatcgcga taccgcgctt
     1741 ttctgaagca cctgaaggct gccatgcaac gaaagtatat tttcccgggg gaatattgtt
     1801 gatacgaaat tttccgtctg cacctgtttt tgcaaaaaac gggttaggca cgacgagaac
     1861 gtcagacacc atgttagagt gaatgttgca gtagatacgc actgccccgg ttttctggaa
     1921 gcggacgttt tttgattttc cggtaccgta gtagtcgagg tcaaacttgt actccggtgt
     1981 gggagaaaaa acgttatggt taaacgggtc ttcgttaggg aattcaacga caccgcccat
     2041 gacgatcggc agcacgcgtg gctgaaactg tttgcccttc tgcacgatct tctcgcgacc
     2101 gttcggattt ggcatctgca gttcttcgag aaagataacg acgtttgagg catcaccttt
     2161 tgcgccctgc ttgccaaaga ggccttttgt tttgacggta acggtaccct caatggtacc
     2221 tcccgcgtgt gcactgctgg cggctacaag caggctgagt gtgagagttg agaatgcttt
     2281 gagaaatttc atcggccctc cggaaaagtc aataggacac gtcagctttt gtggttcggg
     2341 gttacaatct tttttctttg aaatgtggcg aaaactatgc cgcggtagtg cgttcaaggg
     2401 aacccctgtt catgggggtt ttgcgcgaat gaattcccat
//
LOCUS       DAOQJF010000440         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_31752_length_2437_cov_3.937448, whole genome shotgun sequence.
ACCESSION   DAOQJF010000440 DAOQJF010000000
VERSION     DAOQJF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31752_length_2437_cov_3.937448"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLF85_16265"
     CDS             complement(<1..1183)
                     /locus_tag="PLF85_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE21275.1"
                     /translation="MWQKYWPEFLSNYANTHEVRFGKIDEWKLTEAKKLLTCGKFGRG
                     FQWHECESCKVVLAVPFSCKSRLCLSCYRKRLFGWSIQLSKVLNPALLHFHVVFTMPG
                     RLSDLLFARRVNPRFLNLLAARVYAKRQRLTAAVDEAFRPGILSTVHLAGNSLNFNPH
                     VHAIATRDLVNPKTGEMREVNFMPYQTIRYDWQRAVCRYLRRRRLISKDEYDFFLRQY
                     PKGFHVYFQRIAGETSDVIFKTAQYIAFGLFHNSQIENVDHERKTLIFRFKSHVDPQS
                     REKTFSSLTMPIDEFFARMLFFLPDRHEKSIRYYGIYVRPAKRAAVEQAAQMSLWAEG
                     IKCSFDTPNPTACPLCHKNMKTYTIFSPDALRFEKRLRTQYFLADGYFFLKSTRAPPA
                     RS"
     gene            complement(1693..2256)
                     /locus_tag="PLF85_16270"
     CDS             complement(1693..2256)
                     /locus_tag="PLF85_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21276.1"
                     /translation="MNQSASARLYEENPRLRAMIIFILRKLPGKLNRVALCKNLFYTE
                     MHYYQKSQKTISGLEYLHIEASPVPRHFNEIISEMVARKEVRVVPQVAPEVIEGKQIM
                     VLRGMVFECDAPLLNVLGREELRAVRMVSSTLQNEMNLETRYFPQYYQHYVQTGLYEV
                     IPSVVFPKRPHLQFKAWSRKIYRLMWQ"
BASE COUNT          586 a          647 c          613 g          591 t
ORIGIN      
        1 atgaccgggc aggcggtgcc ctcgttgatt ttaagaaaaa atacccgtcg gccagaaaat
       61 actgcgtccg caaccgcttt tcaaagcgca gcgcatcagg ggaaaatatg gtgtatgttt
      121 tcatattttt atggcacaga ggacaggctg ttgggtttgg cgtgtcaaat gagcatttga
      181 tgccttcggc ccagagtgac atctgcgcag cctgctcgac agccgctctt ttcgccgggc
      241 gaacataaat gccatagtaa cgaatagatt tttcatgtct gtccggcaga aagaagagca
      301 ttcgcgcgaa aaattcatct atcggcatcg tcaatgaact gaatgttttc tcccggcttt
      361 gagggtcgac atgactcttg aatctgaata tcagcgtttt tctctcgtgg tcgacatttt
      421 cgatctggct gttgtggaaa agaccgaagg cgatgtattg ggctgtcttg aaaatcacgt
      481 cgcttgtttc gcctgcaatt ctctggaaat agacatgaaa gcctttgggg tactgcctta
      541 agaaaaaatc atattcatct ttcgatatga gccgccgtcg acgcagatat cggcaaactg
      601 cccgctgcca gtcatagcgt atggtctggt agggcataaa attgacttct ctcatttcgc
      661 cggtctttgg gttcacaagg tcgcgcgtgg cgatggcgtg aacgtgcggg ttaaaattca
      721 ggctattacc cgccaggtga acggtactca agatgcccgg cctgaaagcc tcatcaacgg
      781 ccgcagtcag gcgctgtctc ttggcgtaca ctctcgccgc cagcagattc aaaaagcgcg
      841 ggtttaccct tcgtgcaaaa agtaaatccg atagccggcc cggcattgtg aagactacgt
      901 gaaagtgtaa cagggccggg ttcagcacct ttgataactg aatcgaccat ccgaagaggc
      961 gtttgcggta gcatgacaag cagaggcggc ttttgcacga aaacggtacc gccaggacga
     1021 ctttgcacga ttcacattcg tgccattgaa agcctcgccc aaatttaccg caagttaaaa
     1081 gttttttggc ttcagttaac ttccattcgt caatcttacc gaatcttacc tcgtgcgtgt
     1141 tcgcgtaatt cgacaaaaat tccggccagt atttctgcca caacgccttg taggtactat
     1201 gcctggttac ggcgggtcga taagggcgct ggcctgattt cgaacatata tagattgcat
     1261 gcctcagagg gcatacttgc ccttctatat attaaatacc agaaaacagc agatatttct
     1321 catgcttatc ataaaaaatt ctcagaccgc cgaaacatct tttccgttca cttctcaccc
     1381 gtcgcggaac gacctagcgg cccttggcag gggagacaaa accaccccgc ccctacgggg
     1441 cacccctccg aaggagggga atcctgaacc ttcccctccc ccggagggct gttgggttgc
     1501 cgcaggcaac gtggcaggcg tctcgccctg agcgcagtcg aagggagcct gacagggtgg
     1561 tttaccagtt attgccacag tctggcgcct tgccatccgt ggcgcctcaa gtctgctgcc
     1621 caaagcacat cccgtcggct atcgctaacg gctgataact gtgcgcagca gactaaagcg
     1681 catcgtgccc ttttactgcc acatcaatct atatattttt ctactccacg ccttgaactg
     1741 caggtgcggg cgtttgggga acacgacgct cgggatcacc tcgtagaggc ccgtctgcac
     1801 gtagtgctgg tagtactggg gaaaatagcg cgtctcgaga ttcatttcgt tctgcagtgt
     1861 tgacgagacc atgcgcaccg cgcgcagctc ttcgcggccg agcacgttca ggagcggcgc
     1921 atcgcattcg aacaccatgc cgcgcaacac catgatctgt ttgccttcaa tcacctcggg
     1981 ggcaacctgc ggcacaacgc gcacctcttt gcgcgcaacc atctcgctga tgatctcgtt
     2041 aaaatgccgc ggcaccggcg atgcttcgat atgcagatat tctaatccgg aaatcgtctt
     2101 ttgcgacttc tggtagtagt gcatttcggt ataaaaaagg ttcttgcaca gggcaacgcg
     2161 gttcagctta cctggcagct tgcgcagaat aaaaatgatc attgcgcgca ggcgcggatt
     2221 ttcttcgtac aaccgcgcag acgcggactg gttcacattt gaatcgttca acatcgaagc
     2281 aatttttgtc caaaccagag tccaaagctt ttgacacgct gtctcgcaaa taaagaatcc
     2341 cgcccatggc aaaagaactc tacgaactga atgaagcgat ccccgttggg gaagttccca
     2401 agaagatgta cgcaatggcg attcgcaaag aacgcta
//
LOCUS       DAOQJF010000441         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_31917_length_2429_cov_3.780118, whole genome shotgun sequence.
ACCESSION   DAOQJF010000441 DAOQJF010000000
VERSION     DAOQJF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31917_length_2429_cov_3.780118"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLF85_16275"
     CDS             <1..302
                     /locus_tag="PLF85_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21277.1"
                     /translation="LVLAGDAAGQVEARGGSGIRSSFMMGIVSAEAAVDTLLRENFSA
                     QSQSAFKKKVLDCEQMHELKDLNLKFGIPRRLFFSTVTSPSAMDTFWFGLQFFMR"
     gene            complement(340..1449)
                     /locus_tag="PLF85_16280"
     CDS             complement(340..1449)
                     /locus_tag="PLF85_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21278.1"
                     /translation="MTSSALASKLSKLLLLTMTLLAAQSTLHAEATVNRSLALMNPGT
                     STSLRLRDDQVITDVELIATDATSLTYRNQAMTVKIERQDIYRARQGEHFYSDLPLSV
                     ADHEKFPDYVSYIQERGDVIIDLERTQYVGQITAETDALFSIRTRMASYDIAKVKVAA
                     WRRGGKWYGDANAQQQPAGSIFGFQPYQDKARLLNYSLFLTGPWSALPQFGVGVSSNV
                     NHLLFGGLRTSLGYIWLDTASMGILYQVSAHLNLNLFHIGSTRIYAGTSYLWRAGFLM
                     PYGYSNSRTMRNDNVYADITQNIYTIHLGVKYMNLMFEAGVEMPIVRKQRFSRPAAGM
                     ATQAIEIENTVNSLQSAIDRFNDVSRVYASVALMF"
     gene            1553..1873
                     /locus_tag="PLF85_16285"
     CDS             1553..1873
                     /locus_tag="PLF85_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE21279.1"
                     /translation="MVDQITVHDLKEKMDKGEKFVLVDVRNQNELDICKFDNAVHIPL
                     HLLTVRFNELDRDAEIIMTCHHGGRSMQAAHFLVNQGYRNVSNLMGGIDAWASHIDPN
                     MAKY"
     gene            1880..>2429
                     /locus_tag="PLF85_16290"
     CDS             1880..>2429
                     /locus_tag="PLF85_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21280.1"
                     /translation="MRVLCAMSGGVDSAVSAFLLKQQGHEVIGANMRFWEYKEPLPLS
                     PPLDKGRGAEGGVGSCETPVMVKNKLTSCCSPEDMADAERSARGMGIPFYALKMEADF
                     RVNVIDPFIADYENGLTPNPCVHCNTYIKFGEFYEKATALGFDKIATGHYADIVHLPN
                     GRYAVAPAADRKKDQSYYLYGMS"
BASE COUNT          565 a          674 c          654 g          536 t
ORIGIN      
        1 acctcgtgct ggcaggtgac gccgccggcc aggtcgaagc acgcggtggc tcgggaattc
       61 gttcttcttt tatgatgggg atagtttcgg cagaagctgc agtcgatact ctcttacgtg
      121 aaaacttttc agcacagtct caatccgcct ttaagaaaaa ggtactcgac tgcgagcaga
      181 tgcatgagct caaagaccta aacctcaagt tcggcatacc gcgccggttg ttcttttcta
      241 ctgtcaccag cccctcagcg atggatacct tctggtttgg gctgcaattt tttatgcgtt
      301 agtctgctca ggcaaagttc acgataaaat caaagccgat cagaacatca gcgcgacgct
      361 ggcgtaaacc cgcgagacat cgttgaagcg gtctatcgcg ctctgcaggc tgttcacggt
      421 attctcaatt tcaatggcct gggtcgccat gccggcagct gggcgggaga agcgctgttt
      481 acgcacgatc ggcatttcga ctccggcctc gaacatcagg ttcatgtact tcacacccag
      541 gtgaatcgtg tagatgttct gggtaatatc agcataaaca ttgtcgtttc gcatggtccg
      601 tgaattggaa taaccgtaag gcatcaaaaa acccgcgcgc cagagatacg atgtcccggc
      661 atagatacgc gtgctgccga tatgaaacag gttaagattc agatgtgcgc tgacctggta
      721 gagaataccc atcgatgccg tatcgagcca gatgtaaccc agcgaggtac gaagcccgcc
      781 aaaaagcagg tgattcacgt ttgaacttac tcccacgcca aactgtggca atgcagacca
      841 gggcccggta agaaaaagtg aatagttgag caggcgtgcc ttatcctggt acggttgaaa
      901 tccaaaaata gaacccgctg gttgctgctg cgcattcgca tcaccatacc atttgccccc
      961 acgacgccac gcagcaacct tcaccttggc aatatcataa cttgccatgc gcgtacggat
     1021 gctgaaaagc gcatcggttt cagcagtgat ctgcccgacg tactgtgtcc gttcgaggtc
     1081 gataatcacg tcaccccgct cctgtatata agagacataa tcaggaaact tctcgtggtc
     1141 ggccaccgac agcggcaaat cggaatagaa atgctcaccc tggcgggccc ggtagatatc
     1201 ctgccgctcg atcttaaccg tcatggcctg gttgcgatag gtaaggctgg tcgcatcggt
     1261 tgcgatcaac tcgacatcgg taatcacctg gtcgtcgcgc agcctgagac tcgtgctggt
     1321 accggggttc atcagcgcga ggctgcgatt caccgtcgct tcggcgtgca gggtgctctg
     1381 cgcggccagc aaggtcattg tcaaaagcag gagctttgaa agttttgacg caagggcaga
     1441 gcttgtcatg cgtccgcctg aagcgccgcc gagtgggcgt aaacgcaatt tgccggggta
     1501 atcccaccgt gattttggct tacgcgctgt ctcgattgta tattttatcg gtatggttga
     1561 ccagattacc gtccacgacc tcaaggaaaa aatggacaag ggcgaaaagt ttgtcctcgt
     1621 cgatgtgcgc aaccagaacg agctcgatat ctgcaaattc gacaacgcgg tgcatatacc
     1681 cctgcacctg ctgaccgtgc gcttcaacga gctcgaccgc gacgccgaaa tcattatgac
     1741 ctgccaccac ggcggccgtt ccatgcaggc ggcgcacttt ctcgtgaacc agggctaccg
     1801 caacgtgagc aacctgatgg gtggcatcga tgcatgggcg tcgcatatcg accccaacat
     1861 ggcgaagtat taattgagaa tgcgtgtcct gtgtgccatg tcgggcggcg tcgattccgc
     1921 cgtttctgct ttcttgctga aacagcaggg gcatgaagtc atcggcgcga acatgcgctt
     1981 ctgggaatat aaagaacccc tccccctgtc ccctcccctt gataagggga ggggtgccga
     2041 aggcggggtg gggtcgtgcg aaacaccagt catggtgaag aacaagctga cttcttgctg
     2101 ttcgccagaa gatatggccg atgccgaacg ttcggcgcgc ggtatgggta ttccgttcta
     2161 cgcgctcaag atggaggccg actttcgggt caacgtcatc gaccccttca tcgcggacta
     2221 cgagaacggt ttaactccca acccgtgcgt gcactgcaac acctacatca aattcggcga
     2281 gttttacgaa aaggcaacgg cgctcggctt tgacaaaatc gccactggcc actacgccga
     2341 catcgtgcac ctgccgaacg gccgttacgc agtcgcaccc gccgccgatc gcaagaaaga
     2401 ccagagctac tacctctatg gcatgtcgc
//
LOCUS       DAOQJF010000442         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_32522_length_2404_cov_5.401022, whole genome shotgun sequence.
ACCESSION   DAOQJF010000442 DAOQJF010000000
VERSION     DAOQJF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32522_length_2404_cov_5.401022"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLF85_16295"
     CDS             complement(<1..720)
                     /locus_tag="PLF85_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21281.1"
                     /translation="MKSPLLLTALALLTLQVFSRCGQNLDKALLSQQAQSGTQAGATP
                     PVDNGDNANPDPVTPPPGDSGETGTPAVETSTLVAVTSAQVGALGAYVPAQVNGLKGK
                     VIALGAGHGWRGQTSSESAGKRLQRPWVWASGGATYSSAYTTPVNQYAIVEDYLNSEI
                     TWYLNQYLRNAGAETTLVRELARQRQQVAVCPGAAGYAQSGGTFYTSGNDSAQAAQLC
                     GAGTKYRYVYGDNAGNATFNYT"
     gene            complement(717..1433)
                     /locus_tag="PLF85_16300"
     CDS             complement(717..1433)
                     /locus_tag="PLF85_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21282.1"
                     /translation="MPAALRFLLISLLAVYPLVADNLSPGELYDLELPDGSSLNGAVY
                     RGQEAGADSFELSSVVGRIRLKDYRVVPRQKPQPWYVMAAPAYYLPLNQQQLGFSQAL
                     GFNLAGSIPIFSSSNFLTPRAVVAAGFTRYSGSKAMLSGPELTAGPGWLIPLGRTSRF
                     FTVFTLTAGTAFYELLNTNLNQTFSQTTFLGAAELGLGIRLSEWGVICSYVQNYVHDE
                     KLPLVSGGVRLGAVYFGGKA"
     gene            complement(1478..2386)
                     /locus_tag="PLF85_16305"
     CDS             complement(1478..2386)
                     /locus_tag="PLF85_16305"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNE21283.1"
                     /translation="MRQHLAGFIHFLEDERGLAANTREAYQRDLSQWLDFAEREGLAP
                     DEITGEDIYSFLGSLRSRNEPLERRSQARKLSAIKSFYRYLEKRKLAEKNPARTLRAA
                     RYKRLLPRPLRPLELESLLDFSDGEEKFTEMRDRALWETLYSSGMRISEALSLKMQPF
                     SGTQIPDEIKVTGKGNKDRIVFIGSAARHAIAEYLPHRADTLYRTKKQADALFINFKG
                     TALTRRGAHYLLRQRATRLGLDGRITPHSLRHSFATDLLNEGADIRHVQEMLGHASVS
                     TTQNYTHVAKERLFEVYRRAHPHGRT"
BASE COUNT          488 a          692 c          703 g          521 t
ORIGIN      
        1 cgtgtagttg aaagttgcat tgccggcgtt gtcaccgtag acgtagcggt atttcgtgcc
       61 tgcaccgcag agctgcgctg cctgcgctga atcattgcca ctcgtgtaga aagtcccgcc
      121 gctctgcgcg taacctgcgg ctcccgggca gacagcgacc tgctggcgct ggcgggcaag
      181 ctcgcgcacg agcgttgtct ctgcccctgc attgcgcagg tactggttga ggtaccaggt
      241 aatctcggag ttgagatagt cttcaacaat cgcgtactgg ttcaccggcg tcgtataggc
      301 agaagaatat gtcgctcccc cgcttgccca gacccacggc cgctgcaggc gtttgccggc
      361 actctctgag cttgtctgcc ctcgccagcc atgccctgca ccgagagcaa tcaccttacc
      421 cttgaggccg ttgacctgtg cgggcacgta cgcccccaat gctcccacct gggcagaagt
      481 gactgccacg agtgtcgaag tttcaacggc aggtgtgccc gtttcaccgg agtctcccgg
      541 cggcggcgtt accggatcag gattggcgtt gtcgccgttg tcaaccggcg gtgtggctcc
      601 ggcctgggta cccgactgcg cctgctgcga gagcagtgcc ttgtcgagat tttgcccaca
      661 gcgactgaat acttgcaggg tcagcaatgc cagcgccgtg agtaacaagg gggatttcat
      721 gccttacctc caaaatacac tgctcccagt ctcactccac ccgaaaccag tgggagtttc
      781 tcgtcgtgca cgtaattttg cacgtaactg caaatcactc cccattctga tagccgtatg
      841 ccaaggccca gctcggcagc gccgagaaaa gttgtctgcg aaaaagtctg gttcaggttc
      901 gtgttgagta attcgtagaa ggcagtacct gccgtgagcg tgaacacggt aaaaaagcgc
      961 gaagttctgc cgagtgggat aagccagcca gggccggccg taagctcggg tcctgaaagc
     1021 atggccttgc tacccgagta ccgggtaaac ccggctgcga ccacggcgcg gggagtaaga
     1081 aaattcgaac tggaaaagat tggtatactg cccgcgaggt taaagccaag ggcctgcgag
     1141 aaccctagct gctgctggtt gagcggtaaa tagtatgccg gcgccgccat gacataccat
     1201 ggctgcggtt tttgccgggg aaccacgcgg taatctttga gcctgatgcg cccgacgact
     1261 gaagagagtt caaaactgtc ggcacctgct tcttgcccgc ggtagactgc gccgttgagg
     1321 ctgctgccat ccggcagctc gagatcatac aactcgccgg gtgaaaggtt gtcagcgacc
     1381 agcgggtaaa ccgccagtaa gctaatcagg agaaatcgca aggcagcagg cacggccaaa
     1441 gatggtttgt cgcacctgcg ctgtcacgca aaaatattca cgtacggcca tgcgggtgcg
     1501 cccggcggta aacctcgaac aggcgctcct tcgcgacatg ggtgtaattc tgtgtcgtcg
     1561 aaacactcgc gtgaccaagc atctcctgca catgcctgat gtctgcgcct tcgttcaaca
     1621 ggtctgtcgc aaacgagtga cgcaggctgt gcggggtaat gcggccatca agaccaagcc
     1681 gtgtcgcacg ctggcgcagc aggtagtgtg ccccgcgccg ggtaagcgcc gtgcctttga
     1741 agttgataaa cagtgcatcg gcctgcttct tcgtgcggta gagtgtgtca gcgcggtgcg
     1801 gcaggtattc cgcaatcgca tggcgcgctg cgctgccgat aaagacaatg cgatctttat
     1861 tacccttgcc cgttacctta atttcatcgg gaatctgtgt gccgctgaac ggttgcattt
     1921 tcagcgacag tgcttctgaa atcctcatgc cagaagagta tagcgtttcc cacagcgcac
     1981 ggtcccgcat ttcggtaaac ttctcctcgc catcggaaaa atccagcagg ctttccagct
     2041 ctagcgggcg caggggccgg ggcagtaacc ttttgtagcg ggcagcgcgc agcgtacgcg
     2101 ccggattttt ttctgctagt ttgcgttttt caaggtagcg gtaaaaagac ttgatggctg
     2161 aaagtttacg cgcctgcgag cgccgttcca gcggctcatt tctgctgcgc aggctaccca
     2221 ggaaactgta gatatcttcg ccggtaatct catccggggc cagaccttcg cgttcggcaa
     2281 aatcgagcca ctgcgacagg tcgcgctggt acgcttcgcg cgtgtttgca gccagcccgc
     2341 gctcgtcttc gagaaagtga ataaatccgg cgaggtgctg ccgcatcttt tattatcggc
     2401 taag
//
LOCUS       DAOQJF010000443         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_33145_length_2380_cov_4.647312, whole genome shotgun sequence.
ACCESSION   DAOQJF010000443 DAOQJF010000000
VERSION     DAOQJF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33145_length_2380_cov_4.647312"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PLF85_16310"
     CDS             <1..1160
                     /locus_tag="PLF85_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21284.1"
                     /translation="LLFQDLRMITLLQTLGLTLCVFGLRFGLSALPGKAGEFRFTWLH
                     SLHRNPIVLVLVYFGTARGLLPPMAAQLVLWHFLASEILAAVLLKDREPEGGGSAPHV
                     SARVLLPLSNPETMLPLMNLATHLGDLRSPPKLYPLNIVPDDNTAEERIRAVESRFNE
                     IIPMYAAREQPVELTARIENDRIRAVTHAARELLCDRILLGLGAIPTLSRPQGYSFLE
                     NLALNAADRMVIAAHLSSDLSVTGMVNVIVPHARLLESQETWFQALFILANRLRAELT
                     IFGDSEVLASIKAKLEHSGQKRLCHFRAGQIHAGLDLLTLDGSPNTLTVAILARAPLF
                     PQEKIHARLPEMMLRAFADRNFVLLYPPMAAGVQATQSRSAWKKVKRWLGF"
     gene            complement(1195..1956)
                     /locus_tag="PLF85_16315"
     CDS             complement(1195..1956)
                     /locus_tag="PLF85_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21285.1"
                     /translation="MKKILALATLAAFPLALSAYTSTNMAGQTGLISTPSARIAWEGN
                     NSNAAVTAGYSYANNGRTYNAPNVNVALFDRFEMGGVFNAVEGSGNNDFMLHGKLRFS
                     PWSGRGNSALAIGGNYQSLEPGALTVGQVYLAATYEANFFGMEADTSVVLGKTFGSAT
                     RSGDLDFSMGFDLNFFPGVFNGYVRWLNEIANYDYLYGNAPTRAGSRGAFNTGFRIPI
                     LKSMSNLKFDINVKMTDALDDERGFAAGAVFGARF"
     gene            complement(2043..2321)
                     /gene="argB"
                     /locus_tag="PLF85_16320"
     CDS             complement(2043..2321)
                     /gene="argB"
                     /locus_tag="PLF85_16320"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNE21286.1"
                     /translation="MASALGAEKLLLLTDTPGVMHNGETLTRLSPADVRRLKADGVIS
                     GGMIPKTDCAVAAVEAGVKAAHIIDGRVPHALLLEIFTDNGVGTMIIP"
BASE COUNT          528 a          686 c          641 g          525 t
ORIGIN      
        1 gcctgctctt ccaggacctg cgcatgatca ctctattgca gacgttgggt ttaaccctct
       61 gtgtatttgg tctgcgtttt ggcctgtccg cgctgccggg caaagctggc gagttccgct
      121 ttacctggct gcactcgctg catcgaaatc cgatcgtgct ggtcctcgtc tatttcggca
      181 cggcgcgtgg cctgctgccg cccatggctg cacagctcgt gctctggcat ttcctcgcaa
      241 gcgagattct tgcggcggtg ctgctgaaag accgcgaacc agaaggtggc ggcagtgcgc
      301 ctcacgtttc ggcccgggtg ctactgccac tgagcaaccc ggaaacgatg ctaccgctca
      361 tgaacctcgc cacacacctg ggagatctgc gcagtccgcc taaactttac ccgttgaata
      421 ttgtacccga cgataacact gcagaagaac ggattcgcgc tgtcgaaagc agattcaacg
      481 aaattatacc catgtacgcc gcgcgcgaac agccggtaga gctgaccgcg cgcatcgaaa
      541 acgaccgtat tcgtgcagtg acgcacgcgg cgcgcgaact tttgtgcgac cgcattctac
      601 ttggtctagg cgccattcca acgctcagcc gcccgcaggg ctattctttc ctcgaaaatc
      661 ttgcccttaa tgccgcagac cggatggtca tcgcggcgca tctgtcgagc gacctttccg
      721 taaccggtat ggtgaacgtg attgtgcccc atgcccgcct gctcgaaagc caggaaacct
      781 ggttccaggc tctgtttata ttggcgaacc ggctgcgggc cgaactgaca atttttggcg
      841 attccgaagt actggcgtca atcaaggcaa agctggagca ttcaggacaa aaaaggcttt
      901 gccatttccg tgccggccaa attcacgcgg gtcttgacct gctgacactc gatggcagtc
      961 ccaatacgct taccgtcgcc atacttgccc gcgccccgct tttcccacag gagaagatac
     1021 acgcacgcct gcccgaaatg atgcttcgcg catttgctga tcggaatttt gtattgttat
     1081 acccacccat ggctgcaggc gttcaggcaa cacaatcccg ctctgcctgg aagaaagtga
     1141 agcgttggtt aggattttga cacgggcgct gagtgcaccc gtgccgctga aaatttagaa
     1201 ccgggcgccg aagactgcac cagcagcgaa gccccgctca tcgtcgagcg catctgtcat
     1261 cttgacattg atatcaaact tcagattgct catgctcttg agaatcggaa tgcggaagcc
     1321 cgtattgaat gcgccgcgag agcctgcgcg cgtcggggca ttgccgtaga gataatcata
     1381 gttagcgatt tcgttgagcc agcggacata accgttgaac acgccgggaa agaagttcag
     1441 gtcaaagccc atcgaaaaat caagatcgcc cgagcgggtt gcgctgccaa aggttttacc
     1501 gaggacgacc gaagtatcgg cttccatacc gaagaaattg gcttcataag tcgccgcgag
     1561 gtagacctgg cctacggtca gcgcgccagg ctcaaggctc tggtaattgc cgccaattgc
     1621 cagtgcggag ttgccacggc ctgaccacgg ggaaaaccgc agcttgccat gcagcatgaa
     1681 gtcattatta ccactgccct cgacagcgtt gaagactcca cccatctcga aacggtcgaa
     1741 aagagcgacg ttcacgttgg gtgcgttgta agttctgccg ttgttggcat agctgtagcc
     1801 ggccgtgact gcagcgttac tgttgttgcc ttcccaggcg attcgggcgc ttggtgttga
     1861 aatcaggcct gtctgaccag ccatgttggt tgacgtatac gcagacagcg cgagcggaaa
     1921 tgccgcgagt gtcgcgagtg cgaggatttt tttcatagta gtgcagaagc tgatacaccc
     1981 cagccaacgt caaatgcttt agagcacact tgagtacgac acagggaaaa acaattcagg
     2041 cttcagggaa taatcatcgt accgacgccg ttatcggtaa aaatttccag caggagcgca
     2101 tgtggcacgc gtccatctat aatatgggct gccttgactc ccgcttcgac cgcggcgaca
     2161 gcgcagtcgg ttttgggaat cataccaccc gaaatcacgc catctgcttt cagcctgcgc
     2221 acgtcagccg gcgaaagccg cgtcagggtt tcaccgttat gcatcacacc cggggtatcg
     2281 gtcagcaata acaacttctc tgcccccagc gcagaagcaa ttgccccctg cttaattgag
     2341 gcaatatttg agaagaccga ctttctgact tacggtgcag
//
LOCUS       DAOQJF010000444         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_33235_length_2377_cov_4.719208, whole genome shotgun sequence.
ACCESSION   DAOQJF010000444 DAOQJF010000000
VERSION     DAOQJF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33235_length_2377_cov_4.719208"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLF85_16325"
     CDS             complement(<1..408)
                     /locus_tag="PLF85_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNE21287.1"
                     /translation="MDPEVLAEYQKKFPEVFAVIEGVLERFPELDKLLKSQGLVKDQI
                     DAQAKQVLETVAKDMKPYREQYETFRELPKEGRPKEQVLKELREMQAAEIKKWKEGYV
                     SGAVYHGDDSHIEYLNQAYAIHSQSNPLHTDLWP"
     gene            complement(427..2031)
                     /locus_tag="PLF85_16330"
     CDS             complement(427..2031)
                     /locus_tag="PLF85_16330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNE21288.1"
                     /translation="MLRQGSAQVSTDQYVLAIDLGTSGPKVAIVSEMGEVLDCEVEST
                     ELILLPEGGAEQDPVDWWSAICKASQRLIAKNIVPVEQIDCVAVTSQWSGTVAVDAQG
                     KHLYNSIIWMDSRGAKHVSRMMDGLVKFQGYDVVKLAQWIMLTAGMPGLSGKDPLAHI
                     LFIKNEMPDLYARTYKFLEPKDYLNLKLTGKFASAQDAIALHWVTDNRDLNRVYYHPA
                     LIKASGVDQKKLPDLYSSTDVLGAILPEAARDLGISADCKVVLGTPDVQSAAIGSGAV
                     DDFAAHLYIGTSSWLTCHVPFKKTSPEYNMATIPSAIPGRYLIGNEHETAGYCLTYLR
                     DKVFYPKDAITPEGAPPDAYQRFNDLAASVPAGSEKLIFTPWLIGERTPVEDHTIRGG
                     FFNMSLSTTRAHMVRAVFEGVAYNSRWLLEAVEDFAGRKIKALNIIGGGARSDLWCQI
                     HADILNCEVRQVKDAVQANVRGVGLLAGVSLGKFRFKDISSRVAIQKSYQPNPAHRKI
                     YDELYAEFKNVYQAHKDIHKRMNKTH"
     gene            complement(2108..>2377)
                     /locus_tag="PLF85_16335"
     CDS             complement(2108..>2377)
                     /locus_tag="PLF85_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21289.1"
                     /translation="KGKEVVRALDAWATYLDGWKKKANSLEKKKGFKFLKAPEPPEEL
                     KGDVEAFMTSVNTMSQTLQAKVELFKDNPKFNKRIQQVLGKLKDL"
BASE COUNT          512 a          628 c          631 g          606 t
ORIGIN      
        1 cggccacagg tcggtgtgca gcgggttcga ctgcgaatgg attgcgtacg cctgattgag
       61 gtattcaatg tggctgtcat cgccgtggta aacggcgcct gaaacataac cttctttcca
      121 ttttttaatt tcggctgcct gcatctctct gagctctttg aggacctgtt ctttcggccg
      181 gccttctttg ggcagctcgc ggaatgtctc gtactgctcg cggtagggtt tcatgtcttt
      241 cgcgacagtc tcgagtacct gctttgcctg cgcatcgatc tggtctttta ccagaccctg
      301 cgacttcagc agcttatcga gttcgggaaa acgttcgagc acgccttcaa tgacggcaaa
      361 aacttcggga aatttcttct ggtattcggc gagtacttcg ggatccatat ttttctcctt
      421 gatcggttag tgcgtcttat tcatccgctt gtgaatgtct ttatgcgcct ggtagacgtt
      481 cttgaactcg gcgtagagtt cgtcatagat cttccggtgc gcagggttcg gttggtagct
      541 tttttgtatc gctacacggc tggaaatatc tttgaaccgg aacttaccca aagaaacccc
      601 ggcaagcagg cctacgccgc gcacgttcgc ctgcacggcg tctttgacct ggcgtacttc
      661 gcagttgaga atatccgcgt gtatctggca ccacaggtcg ctgcgagcgc caccaccaat
      721 gatgttgagc gccttgatct tgcgaccggc aaaatcttcg actgcctcaa gcagccagcg
      781 cgaattgtag gcgacacctt caaacacggc ccgcaccata tgcgcgcgcg tcgtactgag
      841 cgacatatta aagaaaccac cgcgaatggt gtgatcttca acaggggtgc gctcgccgat
      901 cagccacggc gtaaaaatca gcttctcgct gccagcaggc acagatgctg ccaggtcgtt
      961 gaagcgctgg tacgcgtcag ggggagctcc ctctggtgtg atggcatctt tcgggtagaa
     1021 aactttgtcg cgcaggtacg taaggcaata acctgcggtt tcgtgctcgt taccaatgag
     1081 gtaccggccc ggaatcgccg aaggaatcgt cgccatgttg tactcgggcg aagttttctt
     1141 gaaaggtaca tggcaggtaa gccatgatga agtgccgatg tagaggtgtg cagcaaagtc
     1201 atcgactgca cctgaaccga tcgcggcaga ctgcacgtca ggtgtaccga gtacaacttt
     1261 gcaatccgcg ctgataccca ggtcgcgggc agcctctggt aaaattgcgc cgagtacatc
     1321 ggtcgagctg tagaggtcag ggagtttctt ctggtctaca ccactggcct tgatgagggc
     1381 aggatgatag tacacgcggt tgaggtcgcg gttgtcagtg acccagtgca gcgctatggc
     1441 atcctgcgcc gaggcaaatt tgcccgtcag cttcaggttc aggtagtcct tcggttcgag
     1501 aaacttgtaa gtgcgtgcat acagatcggg catttcattc ttgataaaga gtatatgcgc
     1561 caggggatct ttgcccgaca agcccggcat acccgcagtc agcataatcc attgggcaag
     1621 tttgacgacg tcgtagccct gaaatttcac gaggccatcc atcatgcgcg aaacatgctt
     1681 tgctccgcgc gaatccatcc agatgataga attgtagaga tgcttgccct gtgcgtcaac
     1741 cgccacggta cccgaccact gcgaagtgac ggctacacag tcgatctgtt caaccggaac
     1801 gatatttttt gcaatgagcc tctgcgaagc cttgcagatg gcgctccacc aatcgacggg
     1861 atcttgttct gcgccgcctt cgggcagcag tatcagctca gttgattcaa cttcacagtc
     1921 gagcacctcg cccatttcac tgacaatcgc aaccttgggg ccagaagtac cgagatcgat
     1981 ggcgagaacg tattgatcag tggagacttg tgctgagcct tgtcgaagca tacgtcacag
     2041 cgcgcgatcg accgaaagag tgtaaagcac attgttgccg ttttaccggc aacaatgtga
     2101 gaaactatca gagatccttc agtttaccaa gaacctgctg gatgcgctta ttgaattttg
     2161 gattgtcttt gaagagttct acttttgcct gcagcgtctg cgacatcgta tttacagaag
     2221 tcatgaaggc ctcgacgtca cccttgagct cttcaggtgg ctcgggagct ttcagaaatt
     2281 tgaagccttt ctttttctcc agcgaattcg cctttttttt ccagccgtcg agataagttg
     2341 cccatgcatc gagcgcacgc acaacctctt taccttt
//
LOCUS       DAOQJF010000445         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_33615_length_2363_cov_5.677643, whole genome shotgun sequence.
ACCESSION   DAOQJF010000445 DAOQJF010000000
VERSION     DAOQJF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33615_length_2363_cov_5.677643"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(535..1074)
                     /locus_tag="PLF85_16340"
     CDS             complement(535..1074)
                     /locus_tag="PLF85_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21290.1"
                     /translation="MKETLRKVTSHRIFVPALVGAVALILGLLLAFEHTRNIIKIRGP
                     YLAVLTLISVFPVFAFRFFDPHENSGPITLYFLVSAVYFISLGAPDFVAFLRAILFER
                     VTGEAVKRLLFGFTALGACAGGVHYGLRIWTDKENRPGTFLAISPVKFAVYGILQIIY
                     LNLFVAIARDKFGINTLFG"
     gene            complement(1108..>2363)
                     /gene="eno"
                     /locus_tag="PLF85_16345"
     CDS             complement(1108..>2363)
                     /gene="eno"
                     /locus_tag="PLF85_16345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNE21291.1"
                     /translation="EVIDSRGNPTVEVEVTLDSGDVGRAIVPSGASTGSREALELRDG
                     DKGRYLGKGVLKAVSNVNEIINPHLVGVDGINQREVDRVLLQLDGTENKSKLGANAML
                     AVSMATAHASAKFLDLPLFRYLGSPFSRTLPVPMMNVINGGAHADNNLDFQEYMIVPV
                     AFDSFRECLRQGAEIFHALKKVLHDKSYNTAVGDEGGYAPNVKSNREGLDLLMAAIEK
                     AGYKPGVDTFIAMDAASSEFYKDGKYKLDGDDGKVLDADGIIELYADYCAKYPIVSLE
                     DGMAEQDWAGWKKLTDKLGSKIQLVGDDLFVTNKKILAEGISKGIANSILIKVNQIGT
                     LTETFETMQLAGKHGYTCISSHRSGESEDTTIADLAVAAETGQIKTGSMSRTDRIAKY
                     NQLLRIEDMLGADAFYPGIKAFKNR"
BASE COUNT          545 a          632 c          660 g          526 t
ORIGIN      
        1 agtgctgctg tcaggcaacc atgccaaaat cgccgagtgg agaaaaagcg aatcggaaaa
       61 atttggattg tcggtccaaa ccccacccct accctcccct gaatagggga gggcttttga
      121 acgaaccaaa cccgtggcgg cggaataagc cgtataatgc caggctaacc ggccgtgcaa
      181 aggagatgcg gcataaccct acgtatgctg aagctcagct atggcagctt ttcctgcgat
      241 accatagacc gagattttac aggcagcggc ccatcggtaa ttacatagca cacttctact
      301 gtgctaaggc aaggctagtt atcgagatcg acggcgacgc acacgaagcc acggacatca
      361 aaccatatga ccaatggcgt agcagtgtac tgaatggatt cgggatcatc gttatccgct
      421 tctcgaataa cgatgtggaa aaagcctttg aagaggtatg tgcctcaatc tccgaaaagg
      481 ttgcgacagc actgcgtccc cccctaacag aggggataga ggggggttgt tgcgtcaacc
      541 gaacagggta ttaattccaa atttgtcgcg tgcgatggcg acaaacaggt tgagatagat
      601 aatctgcaga atcccgtaga ccgcgaattt caccggtgaa attgccagga atgtaccggg
      661 gcggttctcc ttatcggtcc agatcctgag gccatagtga actccgccgg cgcaggcgcc
      721 gagcgcagta aaaccaaaaa gcaggcgttt cacagcctcg cctgttaccc gttcgaagag
      781 aatcgcccgc agaaacgcta cgaagtcagg tgcgccgagg gaaataaaat acaccgctga
      841 taccaggaag tacagagtga tcgggcccga gttctcgtgc gggtcaaaga agcgaaaagc
      901 gaagaccgga aagacgctga taagggtcag caccgccagg tacgggccgc gtatcttgat
      961 gatgttgcgc gtatgctcaa aggcgagcag caacccaagg attaaggcca ctgccccgac
     1021 cagcgccggc acgaagatgc ggtgcgaagt tacttttctc agagtctctt tcaaattact
     1081 gcattcccca acagtacacc gttactctca acgatttttg aaagctttga taccagggta
     1141 gaacgcgtca gcgccgagca tgtcttcgat gcgcaacagc tggttatatt tcgcgatgcg
     1201 gtcggtacgg ctcatcgaac cggtcttgat ctggcctgtt tcggcagcga cagcgaggtc
     1261 tgcaatcgtc gtgtcttctg attcgcccga acggtgcgac gagatgcagg tatagccgtg
     1321 ttttccggcg agctgcatgg tttcaaaagt ctcagtcagt gtaccaatct ggttcacctt
     1381 gatcaggatc gagttcgcga tgcccttgct gatgccctcg gcaaggattt tcttgttggt
     1441 cacgaaaaga tcgtcaccca cgagctgtat cttgctgccg agtttgtcgg ttagtttttt
     1501 ccagccggcc caatcttgct ctgccatacc gtcttcgagt gaaacgatgg ggtatttcgc
     1561 acagtagtcg gcatagagct cgataatgcc gtcggcgtcg agcaccttgc cgtcgtcgcc
     1621 gtcgagcttg tacttaccat ctttgtagaa ttcgctcgag gcagcgtcca tcgcgatgaa
     1681 ggtatcgacg cctggcttgt agccggcttt ttcgatcgct gccatcagca ggtcgaggcc
     1741 ttcacggttc gacttcacat tgggagcata accgccttcg tcgccgaccg ctgtattgta
     1801 gctcttatca tgcagcacct ttttcagcgc gtggaaaatt tcggcgccct gccgcaggca
     1861 ttcgcgaaac gaatcaaacg ccacgggaac aatcatgtat tcctggaagt cgaggttgtt
     1921 gtcggcatgc gcaccgccgt tgatgacatt catcatcggc acgggcagcg tgcgcgagaa
     1981 tggtgaaccg aggtagcgaa agagcggcag gtcgagaaat ttggccgatg catgcgctgt
     2041 cgccattgat accgcgagca tcgcgttggc gcccagtttt gacttgtttt cagtgccatc
     2101 gagctgcagc aaaacccggt caacctcgcg ctggttgata ccgtctacgc ctacgaggtg
     2161 ggggttgatg atttcattga cgttcgagac ggcctttagc acacctttgc cgaggtagcg
     2221 gcccttgtcg ccgtcgcgca gctcaagcgc ttcgcggctg ccggtagagg cgccagaggg
     2281 tacgatcgcg cggccaacgt cgccgctgtc gagggtaact tcaacttcaa ctgtggggtt
     2341 gccgcgcgag tcgatcactt cac
//
LOCUS       DAOQJF010000446         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_33826_length_2355_cov_4.827391, whole genome shotgun sequence.
ACCESSION   DAOQJF010000446 DAOQJF010000000
VERSION     DAOQJF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33826_length_2355_cov_4.827391"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="pip"
                     /locus_tag="PLF85_16350"
     CDS             complement(<1..719)
                     /gene="pip"
                     /locus_tag="PLF85_16350"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNE21292.1"
                     /translation="MNSPELKQLYPEITRVKSYRLKVSDLHTIYVEECGNPKGIPALF
                     LHGGPGAGLSLNHRRFFNPEKYRIILFDQRGAGKSTPYAELRENTLWDLISDMEVIRQ
                     KLRVSQWLVFGGSWGSTLALTYAISHPERILHLVVRGIFLCRHEEILWFYQYGAHQIF
                     PDAWQNYVKVIPENERHDMLSAFHRRLTSTDKKVRLEAAKAWSQWEGATICLRPSDSV
                     YHEFTEIAVSLARIECHYFVN"
     gene            complement(790..1563)
                     /locus_tag="PLF85_16355"
     CDS             complement(790..1563)
                     /locus_tag="PLF85_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNE21293.1"
                     /translation="MAGPERIVCLTEETTEWLYLLGEERRVVGVSAYTVRPPQARQKP
                     RVSAFLTGNIKKIKSLKPDLVIGFSDIQSELAAKLIKEGLNVLVTNQRSIAEIESTLR
                     LFAGIVGQSKRGESLLKAWNKKLAAIRQRNLGQRKVRVFFQEWDEPVISAISWVSELI
                     EICGGRNIFAKRSGAMAKDRIVTVEDVLKARPEVIIGSWCGKAMDRKWLGTNLSSTPA
                     MRNGQVYEVDSAEILQPGPALFLDGIDRLERAISAARAL"
     gene            complement(1550..>2355)
                     /locus_tag="PLF85_16360"
     CDS             complement(1550..>2355)
                     /locus_tag="PLF85_16360"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21294.1"
                     /translation="YQISEAGEKPSARALQRQYEEILHSPVGVTFRLFDFSEDKNFLA
                     HPLIERDRELRGVRFLLKEKRILETQLTALVRAAQQVQKKIRVLIPYLSEWHELAALR
                     DLLNSVLPGKNVPVISLGAMLETASGVLNARRWADKLDFIYVGTSDLLSAIAAERREN
                     SAALHRALLSDAFVAFARELRFLARRKPLTICGEIAGEPWAIAWLAAHGFRDYTMPTH
                     RMAVVTNMLQALSRTQCSQFARAVLKIEDESQRVETAKLLALDILYGRS"
BASE COUNT          478 a          654 c          685 g          538 t
ORIGIN      
        1 ttgttcacga agtaatggca ttcgatgcgc gcgagcgata cggcgatttc ggtgaactcg
       61 tggtagacac tgtcgctggg tctgaggcag attgtcgcgc cttcccactg gctccaggct
      121 ttggcggctt cgaggcgtac tttcttgtcg gtcgaggtca ggcggcggtg gaatgcactg
      181 agcatgtcat ggcgttcgtt ttccggtatc accttcacat agttctgcca ggcgtcgggg
      241 aaaatctggt gcgcaccgta ctggtagaac cagagaattt cttcgtggcg gcagagaaag
      301 ataccgcgca cgaccaggtg gagtatccgc tcggggtggc tgatcgcgta cgtgagtgcg
      361 agtgtactgc cccacgaacc gccgaacacc agccactgcg aaactctgag tttctggcga
      421 ataacttcca tgtcgctgat gagatcccac agcgtgtttt cgcgcagctc ggcatacgga
      481 gtgcttttgc ctgcaccgcg ctggtcgaag aggatgatac ggtatttttc aggattaaaa
      541 aaacgccggt ggttgagcga caagccggcg cccgggcccc cgtgtaaaaa cagcgctgga
      601 attcccttcg ggttgccgca ttcttcgaca tagatcgtgt gcaggtcaga gaccttcagg
      661 cggtacgact tgacgcgggt aatttcgggg tagagctgtt tgagttcggg ggagttcatt
      721 ggttatggcg gcaatgcaca aaattttagg ctgtagtaaa gctttatagc caccgaattc
      781 attcggtggc tataaagccc gggctgccga tatcgcccgc tcgaggcggt caatgccatc
      841 gagaaagagg gcagggccgg gctgcagaat ttcggccgaa tcgacttcat atacctggcc
      901 gttgcgcatc gcgggggtgc tggagaggtt agtcccgagc catttccggt ccatggcttt
      961 cccgcaccac gagccgatga taacttcggg gcgcgccttc aggacatctt caactgtgac
     1021 aatgcggtct tttgccatag cgcctgagcg cttcgcaaag atattcctgc cgccgcagat
     1081 ctcgatcagc tccgataccc agctaatggc cgagatgacc ggctcatccc attcctggaa
     1141 aaagacccta accttccgct gcccgagatt gcgctggcgt atggcagcga gcttcttgtt
     1201 ccacgccttc agcagtgatt cgcctctctt gctctgccca acgataccgg caaatagccg
     1261 cagcgtcgat tcgatctcgg cgatactgcg ttggttcgtc acgagaacat ttaatccttc
     1321 tttaataagt tttgcggcga gttcggattg gatgtccgaa aagccaatca ccaggtcggg
     1381 tttgagtgat tttatcttct taatgttgcc ggtcagaaat gcgcttaccc tgggtttctg
     1441 acgcgcctgc ggtggccgca ccgtatacgc gctgactccc acaacacggc gttcttcacc
     1501 gagcaggtag agccactcgg tcgtttcttc ggtcaggcag acgatgcgtt caggacctgc
     1561 catagagaat atcaagcgcc agcagcttgg cggtctcgac acgctgcgat tcgtcttcga
     1621 tcttcagcac agcacgggca aactggctgc actgcgtacg cgagagcgcc tgcagcatgt
     1681 ttgtcactac tgccatgcgg tgtgtcggca tggtgtaatc gcgaaagccg tgcgccgcca
     1741 gccaggcgat tgcccacggt tcgccggcga tttcgccgca gatggtcagc ggcttgcggc
     1801 gggcgaggaa gcgcagctcg cgcgcgaagg caacgaacgc atcgctcagc agtgccctgt
     1861 gcagagcagc gctgttctcg cggcgctcgg cagcgattgc gctcagcagg tcggaagtac
     1921 cgacgtaaat aaagtccagc ttgtcggccc agcggcgcgc attcagaaca ccgcttgccg
     1981 tctcgagcat agctccgagg ctgataaccg gtacgttttt tccgggtaat accgagttca
     2041 ataagtcgcg gagcgccgcg agctcatgcc attcagaaag gtaggggata agaacccgta
     2101 tttttttttg cacctgctgc gccgcgcgga cgagagcagt gagctgcgtc tccagtatcc
     2161 ttttttcttt caggagaaaa cgcacacccc gtaattcgcg gtcgcgttca atcagcgggt
     2221 gcgcgagaaa gttcttgtcc tcggaaaaat caaacaaacg gaatgtcact ccaacgggtg
     2281 agtgcagtat ctcttcgtac tggcgctgca acgcgcgcgc cgaaggcttt tcccccgcct
     2341 cactaatctg gtact
//
LOCUS       DAOQJF010000447         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_34128_length_2344_cov_4.470511, whole genome shotgun sequence.
ACCESSION   DAOQJF010000447 DAOQJF010000000
VERSION     DAOQJF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34128_length_2344_cov_4.470511"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLF85_16365"
     CDS             <1..601
                     /locus_tag="PLF85_16365"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNE21295.1"
                     /translation="VPDGDCYRGSARAFKNENVHALIDRLKDLLLQYGGHRQAAGFSV
                     SAEKLTAFVDALFAAAPEIFSPAGGESAESVDPFRNFLPALEIETQHMRIPLWDELLT
                     HAPYGVLNPHPVLAVKRTGKIEVANLGKTGAHAVVRFSAAADRSIEGVWFFHNGEARG
                     AAGQENLTISGEPQVGKFMGRTQYRLKITRVVAPESTSD"
     gene            complement(623..1318)
                     /locus_tag="PLF85_16370"
     CDS             complement(623..1318)
                     /locus_tag="PLF85_16370"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE21296.1"
                     /translation="MQQKKTYSFRGKEGDIGGDFCLLTTLQFSGHKGDWLFFFNGDAM
                     GKSSQGASGALICGVTLHSILGRNARGQEISAEPQHYLYRIYKELNQIFVLFDGSMLM
                     SAAFGLVHSATGTVFYMNCEHPWSILYRNGQAKFIEGDLMLRKLGMIGFDDKPRIRRF
                     ELSPGDTLVVGSDGRDDLEIDGTVDSDETRFLRIVEQTGGDINRVPDQLDQLGRRTDD
                     LSLVAITYERQVN"
     gene            complement(1233..>2344)
                     /locus_tag="PLF85_16375"
     CDS             complement(1233..>2344)
                     /locus_tag="PLF85_16375"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE21297.1"
                     /translation="IAESVNVGPILRVANQVATSVYLDQITVYDKKGVVVMRSHNTAE
                     FGGDESKSEHIAKALRKGENTANLVYTNGNLVLQNTVPVYFDSEIVGAVTAGYILGHK
                     FARSLSDLTQAGIFFVLNGRVASGSFLAKPPGGDKALYEEENRDYTLSRSVPVRMQGD
                     KVEYGRFEFRYIPILTQPGSPDITRVGIAVAVKPPFSRLLMFTLFWGTFLLSITIVLF
                     GIVLAFKVGHNIAGYASTISTAMSRFAGGQMTERVMQESADELGSVARGFNQLAEELQ
                     RKISEIREANENLEIKVEQRTRDLNQALSDVTQRNAGRRLPADVAAGAPAGRIGLPYN
                     KYRCTVSDAAEENLQLSRQRGRYRRRFLPADHPAV"
BASE COUNT          504 a          661 c          654 g          525 t
ORIGIN      
        1 ggtacccgac ggcgattgtt accgcggctc tgcccgggca ttcaagaacg aaaatgtaca
       61 tgcgctcata gaccgcctga aagatctcct gcttcaatac ggcggccacc gacaggcagc
      121 gggtttttcc gtctcagcgg aaaaactcac cgcatttgtc gatgcactct ttgccgctgc
      181 gcccgaaatt ttttccccgg ccggtggcga atcagccgag agcgtcgacc cgttccgtaa
      241 ttttttaccc gcgctcgaaa ttgagacgca gcacatgcgc attcccttat gggatgagtt
      301 gctgacacat gcgccgtacg gtgtgctcaa cccgcacccg gtgctcgcgg tgaagagaac
      361 cggaaagatt gaggtcgcaa atctcggcaa aacgggcgcc cacgcggtag tgcggttcag
      421 cgctgccgcc gaccgcagta ttgaaggtgt atggtttttt cacaacggcg aagcccgcgg
      481 tgcggcaggc caggaaaatc tgaccatcag cggcgaaccg caggtcggta agtttatggg
      541 gcgcacccag tacaggctga aaatcacgcg ggttgtcgcg cccgaaagta cttctgattg
      601 atttctatct atcgaggtgc ggttaattaa cctgtcgttc gtaggtgatg gctacgaggg
      661 agaggtcatc agtccgccga cccagctggt ccagctggtc aggtacccgg ttaatatcgc
      721 cgccggtttg ttctacgatg cgcaggaacc gcgtttcgtc tgaatccacg gtcccgtcaa
      781 tttccaggtc gtcgcgaccg tctgaaccta ccacgagcgt gtcgccaggg ctgagctcga
      841 accggcgaat gcgcggttta tcgtcaaagc cgatcatacc cagtttgcgc agcattaggt
      901 cgccctcgat aaattttgcc tgcccgttgc ggtagaggat actccatggg tgctcgcagt
      961 tcatgtaaaa gactgtgccg gtggccgagt gcaccagccc aaacgcggca gacatcagca
     1021 tcgaaccatc gaaaagcacg aagatctggt ttagctcttt gtagatgcgg taaaggtaat
     1081 gttgcggttc ggcactgatt tcctgcccgc gggcatttcg gcccaaaata gagtgcagtg
     1141 taaccccaca gatcagcgca cccgaagccc cctggcttga tttacccatc gcgtcgccgt
     1201 taaagaagaa cagccagtcc cccttgtgcc cgctaaactg cagggtggtc agcaggcaaa
     1261 aatctccgcc gatatcgccc tctttgccgc gaaagctgta ggttttcttc tgctgcatca
     1321 gaaactgtgc agcgatattt gttgtacggc aatccgatac gaccagcggg cgcaccagca
     1381 gcgacatcag caggtaatcg ccttcctgca tttctttgag tcacgtccga cagcgcctgg
     1441 ttaaggtcgc gcgtgcgttg ttctacctta atttcaaggt tctcattggc ctcgcggatt
     1501 tcactgatct tgcgctgcag ctcttcggcc agctggttaa aaccgcgcgc caccgaaccg
     1561 agttcgtcgg cagattcctg catgacgcgt tcggtcattt ggccgccggc gaaccggctc
     1621 atcgcggttg agatcgttga agcataaccg gcgatgttgt gtcccacttt gaatgcgagt
     1681 acaatgccaa aaaggacaat cgtgatactt aaaagaaaag tgccccaaaa cagagtgaac
     1741 atcagcaggc gcgagaaagg cggcttaacg gcaaccgcga tgccgacgcg ggtgatgtca
     1801 gggctgcccg gctgggtaag aatcggtatg tagcgaaact cgaacctgcc gtactcgact
     1861 ttgtccccct gcatccggac gggcacgcta cgcgagaggg tgtagtcgcg gttctcttct
     1921 tcatagagcg ctttgtcgcc accaggtggt ttcgccagga atgatccgga agcgacgcgg
     1981 ccgttgagaa caaagaaaat tccggcctgt gtcagatcag atagtgaacg cgcaaatttg
     2041 tgcccgagaa tatagcctgc tgtgaccgca cccacgattt cactgtcgaa gtagacaggc
     2101 acggtattct gcaatacgag gttgccattg gtgtacacca ggttggctgt gttttcgccc
     2161 ttgcgcagcg ccttggcaat atgctccgac ttgctttcgt cgccgccgaa ctctgcggtg
     2221 ttgtggctgc gcatgacgac cacacccttt ttgtcgtaaa cagtgatctg gtctaggtat
     2281 acgctggtgg caacctggtt cgccacgcgc agaatcgggc ctacgttcac gctctcggca
     2341 atca
//
LOCUS       DAOQJF010000448         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_34375_length_2335_cov_3.348684, whole genome shotgun sequence.
ACCESSION   DAOQJF010000448 DAOQJF010000000
VERSION     DAOQJF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34375_length_2335_cov_3.348684"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLF85_16380"
     CDS             complement(<1..925)
                     /locus_tag="PLF85_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21298.1"
                     /translation="MRPFGLALLVLCLSPLAATPTVKRLLVLAPVQEGNSADNAYIGP
                     SLAEAIRSKLSEQFFFFHPDDEAIVQTQKNNFIQDEDLYTKSAAIQMGEWLRQDLVMN
                     GKYSVAGNRLRLNLNVYEIESGRMIVNFRTEAPLSANMFETFNKIALELGEIMAQALP
                     SQQELKASGGTYYDPEKGKRTVLLAFGMRAAGFSEVATNLSSSATVSSADFAYFSAAL
                     TYQRHSVLDAFNRRYKWPIFAQVSLHGGYGRRDYNRDGQAVPVRLIAGELVPALGYSF
                     MLRWFRLEPFAGFGLGYSNFQLDYSGLARKPV"
     gene            1039..1488
                     /locus_tag="PLF85_16385"
     CDS             1039..1488
                     /locus_tag="PLF85_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21299.1"
                     /translation="MIYTRLFSILRVPLGYSAYLLLYTQLAAQIPGQVFLTDILRKGL
                     ANPSVTFASSAESVPPKHYPVYLRFKGKHCKTCRFEINAAWINGKRLKHGKLSWEFES
                     LSGEKPYLLKIDVDELWDGKVVRSRVLYGDLTTQAFTEVISLDLVVP"
     gene            complement(1468..2103)
                     /locus_tag="PLF85_16390"
     CDS             complement(1468..2103)
                     /locus_tag="PLF85_16390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE21300.1"
                     /translation="MQAVKRKQQQNPVSSPVPQKPSARDRIVTTAAQLFEAQGYAATG
                     INQIIAESGTAKASFYDYFASKELLGAEYLAVYGAHHLALIRNLTERSATPRQFLSSW
                     VKLIRRQLRERQLYGCPMANLRAQIGDESPLLIAKVRELTQATITLLADYVRQCGIVC
                     DQKGALLAARRVFAAYEGAIHIWKLSGDEAALGDIEDLALAALNLSGNNQV"
BASE COUNT          508 a          691 c          655 g          481 t
ORIGIN      
        1 cgaccggctt gcgtgcaagg cccgaataat cgagctggaa attggaatag ccgagaccga
       61 agcctgcgaa cggctcgagc ctgaaccagc gcagcatgaa actgtaaccc agcgcaggca
      121 ccagctcacc cgcaatgagc cgcaccggga cagcctgccc gtcgcggttg tagtcgcgcc
      181 tgccgtaacc accgtgcagg cttacctgtg caaagatcgg ccacttgtag cggcgattaa
      241 aagcatcgag cacactgtgc cgctggtagg tcagtgcggc actaaagtaa gcgaagtcgg
      301 cactcgacac ggtggcagaa ctgctcaggt tcgtggcaac ttcagaaaag ccggcagcgc
      361 gcatgccaaa cgccagcaat actgtgcgct tgcctttttc aggatcatag tacgtgccgc
      421 cgctggcctt gagctcctgc tgcgaaggca gcgcctgcgc catgatctcg cccagttcga
      481 gtgcgatttt gttgaaagtc tcgaacatgt ttgccgagag cggagcttcg gtgcggaagt
      541 tgacgatcat gcgcccgctt tcgatttcgt atacattgag attcaggcgc aggcggttac
      601 ccgcgaccga gtacttacca ttcatgacca ggtcttgccg cagccattca cccatctgta
      661 tcgctgcaga cttggtatag agatcttcgt cctggataaa gttgttcttc tgcgtctgca
      721 caatcgcttc gtcgtcgggg tgaaagaaaa agaactgctc ggagagcttc gacctgatgg
      781 cttcggccag cgacgggccg atataagcat tgtcggcgct attaccttct tgcaccggcg
      841 caagcaccaa caggcgcttg accgtcggcg tcgcagcaag cggacttaag cagagaacaa
      901 gcagggctag cccgaatggc cgcataccgt tctggctacc acatttttct ctgcgtcaag
      961 cagattcgta actcatgatt cttgttgccg ttctgatcag ctgttacacg cgcagctcat
     1021 tacctacgaa atcagcctat gatctacaca cgtctatttt ctatcttgcg tgttccgctg
     1081 ggatattctg cctacttgct actttacacg caactcgcgg cgcagattcc gggacaggtt
     1141 ttcctgaccg atattctgcg caagggactg gcaaaccctt cggtcacctt tgccagttcg
     1201 gcagaatcgg taccgccgaa acactaccct gtgtacctgc gcttcaaagg taaacactgc
     1261 aagacctgcc gctttgaaat caacgccgcg tggatcaacg gcaagcgcct gaagcacggc
     1321 aaactatcgt gggagtttga gagcctgagc ggcgaaaaac cctacctgct caagatcgac
     1381 gtcgatgaac tgtgggatgg caaagtggta cgcagccgcg tactctacgg cgacctgacc
     1441 acgcaggcgt tcaccgaagt gatctcgtta gacctggttg ttccctgaga ggttgagcgc
     1501 cgcgagcgcg aggtcttcaa tgtcaccaag cgcggcttcg tcaccgctca gtttccagat
     1561 gtggattgcc ccttcgtacg cggcaaaaac ccggcgcgca gcgagcagcg cacctttctg
     1621 gtcacacacg atgccacact gcctgacata atcggcgagc aacgtaatcg tcgcctgcgt
     1681 cagctcgcgc accttggcaa tgagcagcgg cgattcgtcg ccgatctgcg cacgcaggtt
     1741 ggccatcggg caaccgtaga gctgccgctc gcgcaattgc cgcctgatca gcttcaccca
     1801 tgacgacaga aactgccgcg gcgttgccga gcgttcggtc aggttgcgta tcagcgccag
     1861 gtggtgtgcg ccatagacgg caaggtactc ggcaccaagc agctccttcg aagcaaagta
     1921 gtcgtagaag ctcgccttcg ccgtacccga ctcggcgatg atctggttga tgccggtggc
     1981 tgcatagccc tgcgcctcga aaagctgcgc agcggttgtg acgatgcggt cgcgcgccga
     2041 aggtttctgc ggcacggggg agcttaccgg gttctgctgc tgttttctct tgacggcctg
     2101 catcgaagtt tgcgacagac agacttgtct gtacggaatg tttgcccggt cgccacgcgc
     2161 cggcaagcgt tattcgctcg atgcgccgca cagcgggaga aagaatcatg gaaccaaccg
     2221 cagaagcaac cgatttcgcc gacatcacgg gcaacgcgaa gagcgtcagc gacgcactca
     2281 acacacgcca cagcatacgc cggtttgaca cgcggcgcgt tgaagcagaa aaact
//
LOCUS       DAOQJF010000449         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_34390_length_2334_cov_4.903905, whole genome shotgun sequence.
ACCESSION   DAOQJF010000449 DAOQJF010000000
VERSION     DAOQJF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34390_length_2334_cov_4.903905"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLF85_16395"
     CDS             complement(<1..442)
                     /locus_tag="PLF85_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21301.1"
                     /translation="MNIAFEHACRQCGQVFSPQVDLSRYEILTARCPGCGHSHGYDNR
                     AGKLSALVAQRLGQPVIQPSVAAVPHAEEPSSVAALPEPATTHTAVPQLVAEQHATWE
                     SESPWTQPSTNAAAEEPVRAARSHNWPAFRFRLPGIRLAVLITAG"
     gene            complement(488..1210)
                     /locus_tag="PLF85_16400"
     CDS             complement(488..1210)
                     /locus_tag="PLF85_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21302.1"
                     /translation="MRKSLIILTSLAALAVCSVLWGQKIIDPQVIAQQEEQKKRADES
                     GYGDIEIDNLHREIAMLAPKAAPRMKKTEDGGIALEIASGESIKTISERYLYNGTIIL
                     YTGGDEKSPKLNKIKLVFERTNPFGKEYKREKREVLNPTPLFFDNPEQKEGQRSEPKV
                     DRNDDMQLTYFESEFDARQQETFREKEKVELKTIPFYEKKIDFVETYKKYLRRTAKSL
                     RFTVESIEISQKKKLRDLFELK"
     gene            complement(1340..>2334)
                     /locus_tag="PLF85_16405"
     CDS             complement(1340..>2334)
                     /locus_tag="PLF85_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE21303.1"
                     /translation="NDYADPNYTDKDLQTPLLIASAYGNYDMVKTLLERGAQVHARDK
                     DGYSAAFYAAQENRVRILDELIRRKTDLVRKNNFGYTPLILAAAKGHVEAVRRLVEAG
                     VDVNATADNGGTAAYWAAIKDHDEVVKILVGAGAVDPKNNKELVLAARTNDVERAERA
                     LQANINIDLKNEDGYTALMYAVNTGSMDVARVILSRKARINLQDRDGYSALMIAAFRG
                     NAEMLDFLLQYKPDVNLRHRSGRTALMMAAWKGNVSMVQKLLAAGARVNEQDLDGWTA
                     LMFSAHAGDLEKVRLITGARANLALRNKKGQTARELAESRNAADVAEFLAAKKG"
BASE COUNT          468 a          654 c          605 g          607 t
ORIGIN      
        1 aacccgcggt gatcaggacg gccaaacgga tacctggcag cctgaaacga aacgccggcc
       61 aattgtgtga cctggccgct cttacaggct cttcagctgc tgcatttgtc gatggttgcg
      121 tccagggtga ctcgctctcc catgttgcat gctgttctgc tacgagttga ggtacggcgg
      181 tgtgtgtcgt cgcaggttcc ggtagtgcag caacagacga cggctcttca gcatgaggca
      241 cagccgcaac tgacggctga ataacaggtt gcccaaggcg ttgtgcaacc agggcagaaa
      301 gtttaccagc ccggttatcg taaccgtggc tatgcccgca accgggacag cgtgcggtga
      361 gtatttcgta gcggctgagg tctacctgcg gcgaaaacac ctgcccgcat tgacggcagg
      421 catgctcgaa ggcaatattc attgccggca ccaacgtaac ttcccgttca ggccggctgg
      481 aagcatacta cttcagttca aacaggtcgc gcagcttctt cttctgtgaa atttcaatcg
      541 actctacggt gaagcgcaac gactttgcgg tgcggcgcag gtatttttta taagtctcga
      601 caaagtcgat tttcttttcg tagaatggaa tggttttcag ctccaccttc tctttttcac
      661 ggaaggtttc ctgctggcgg gcgtcaaatt ccgactcgaa ataagtaagc tgcatatcgt
      721 cgttgcgatc gactttcggt tcgcttctct gcccttcttt ctgttcggga ttgtcaaaga
      781 ataacggggt tgggttaaga acttcgcgtt tttcccgctt gtattccttg ccgaagggat
      841 ttgtgcgctc aaacacaagc ttgatcttgt tcagttttgg ggatttttca tctcccccgg
      901 tatacaggat gatggttcca ttatagaggt aacgctccga aatggtcttt atagattctc
      961 ctgaggcaat ctcgagcgca ataccgccat cttcggtttt tttcatacgc ggcgcggctt
     1021 ttggtgccag catcgcaatc tcgcggtgca ggttgtcgat ttcaatgtcc ccgtagccag
     1081 attcatctgc tcgttttttc tgttcttcct gctgggcaat gacctgagga tcgatgattt
     1141 tctgtcccca aagcacgctg cagaccgcaa gagcggccag agaggttaag ataataagag
     1201 atttgcgcat gtattccccg tttggccctg agagccctgt cttaattaac ggccagccga
     1261 gggggtgtcc tttaagcaga caggcaaaaa agtctgccca ttgccaggca tgctaaccgc
     1321 ggcttataca ggcaacttgt cagcccttct ttgcagccag gaactccgcc acgtctgcag
     1381 cgtttctgga ctcggccagc tcgcgcgccg tctgcccctt tttattccgc agcgccaggt
     1441 tcgccctggc acctgtaatc aggcggactt tttcaagatc acccgcgtgg gcagaaaaca
     1501 tcagggctgt ccaaccgtcg agatcctgct cgttgacacg tgcacctgcc gcgagcagct
     1561 tctgcaccat cgatacattg cctttccacg cggccatcat cagcgcggtc cttccgctgc
     1621 ggtggcgcag gttaacgtcg ggtttgtact gcagcaggaa atcgagcatc tccgcattac
     1681 cccggaatgc agcaatcatc agcgctgagt atccgtcacg atcctgcagg ttaatgcgcg
     1741 ccttacgcga aagaattacc ctggcgacgt ccatcgaccc ggtgtttacc gcgtacatca
     1801 gcgcagtgta accatcttcg tttttcaggt caatgttgat gttggcctgc agtgcccgct
     1861 cggcgcgttc gacatcattg gtacgcgctg ccagcaccag ctctttgttg ttcttcggat
     1921 caactgcgcc tgccccgacg agaatcttaa ccacttcatc atgatctttg atcgccgccc
     1981 agtacgcagc cgtaccaccg ttatctgcag tggcattgac gtctacaccg gcctcgacta
     2041 agcggcgtac cgcttctacg tggccttttg ccgcagcgag aattaatggc gtataaccaa
     2101 aattattttt gcgcacgaga tcggtcttgc gcctgatcag ctcgtctaga atgcgtaccc
     2161 ggttttcctg cgcagcatag aatgctgcag agtagccgtc tttatcgcgc gcgtgcacct
     2221 gcgctccccg ctcgagcagg gtctttacca tgtcatagtt gccataggcc gaggcgatca
     2281 gcaatggagt ctgcaggtct ttgtccgtgt agttcggatc tgcatagtca ttca
//
LOCUS       DAOQJF010000450         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_34778_length_2320_cov_4.115232, whole genome shotgun sequence.
ACCESSION   DAOQJF010000450 DAOQJF010000000
VERSION     DAOQJF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34778_length_2320_cov_4.115232"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..851
                     /locus_tag="PLF85_16410"
     CDS             189..851
                     /locus_tag="PLF85_16410"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNE21304.1"
                     /translation="MQKTKGTPPTAKPKFVKRPAFFFAGFAAIALAAGTFAAYPKWQK
                     QETENWVYTYVTQHFPGKENTSLQQLSRIIYREAMKLRLDLASDAHQYDRAALLMGVI
                     QTESEFNRKARSPVGARGFMQLMPATAALLKGRRLSQEELTDADTNITLGVRYLNDMI
                     REMGSVEKALLAYNAGPGAVRKWGGVPAYYTHVMRAHHTFLAYRAEGRTPRLEIAQLV
                     ED"
     gene            complement(859..1353)
                     /locus_tag="PLF85_16415"
     CDS             complement(859..1353)
                     /locus_tag="PLF85_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE21305.1"
                     /translation="MPTIIRYRPEEQQHDTAIVGLLQAAYVDAGFTAPEIAARMFQPD
                     EVKKRGEVLLALSDETNVIGMIIVATDSNPYRQVAGSGEAEMQLLATHPASRTRGVGE
                     ALCRAFETRAVERGLSSAVLSTQPTMHAAHRLYLRMNFVRNPARDWRRNDRQFWVFDK
                     TLRV"
     gene            complement(1371..1856)
                     /gene="purE"
                     /locus_tag="PLF85_16420"
     CDS             complement(1371..1856)
                     /gene="purE"
                     /locus_tag="PLF85_16420"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNE21306.1"
                     /translation="MAAKVAIIMGSESDWKVMQAAQEVLKELGVAAHAEVVSAHRTPE
                     KMFGFAKEAKKNGYHVIIAGAGGAAHLPGMVASLTTLPVIGVPVKSSNSLDGWDSILS
                     ILQMPGGIPVATVALENAKNAGLLAAQILALSDEVLAQKLAQYRTALADKVHASKLPT
                     V"
     gene            complement(1915..>2320)
                     /locus_tag="PLF85_16425"
     CDS             complement(1915..>2320)
                     /locus_tag="PLF85_16425"
                     /inference="COORDINATES: protein motif:HMM:NF037629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE21307.1"
                     /translation="SLGIVGLIAVEMFLTERGEILINEMAPRPHNSGHHTIEAAATSQ
                     YENLVRILTGLPLGSPETTAPSLMINLLGPESDRVAQWNAMIQTALKMPNAQVHLYGK
                     RDMRPYRKLGHITLVGERNTLIRQYETLTFGA"
BASE COUNT          488 a          694 c          662 g          476 t
ORIGIN      
        1 cgtctatttc tgcctacctt cgaaccatag tgcatcgcac caaatcgcaa gatttggcca
       61 cggggcgaac cagacggcac aggccatcgg atacacccca aggagacggc atgagagcca
      121 acgcgtttgc ctgcaaacgc ccagactgtc cgccgtctcc aagatacctc gtgtatcaca
      181 ggaggattat gcagaagacc aaaggaacac cgccgaccgc gaaaccaaaa ttcgttaaga
      241 gaccggcctt ctttttcgcc ggttttgccg ctattgcgct ggccgccggc accttcgccg
      301 cctaccccaa atggcagaag caggagactg aaaactgggt ttatacatac gtcacccagc
      361 actttcccgg taaagagaac accagcctgc agcaactttc gcgcatcatc taccgcgagg
      421 ccatgaagct gcgcctcgat ctcgcgagcg acgcgcacca atacgaccgc gccgcgctgc
      481 tcatgggagt gatacagact gaaagcgaat ttaaccgcaa ggcgcgctca cccgtgggtg
      541 cccgcgggtt catgcaactg atgccggcaa ccgccgcgct gctgaaaggc cgtcggctgt
      601 cacaggaaga actgaccgac gccgacacga acatcacgct cggcgtgcga tacctgaacg
      661 acatgatccg cgagatgggc agcgtcgaaa aagccctgct ggcctacaat gccggcccgg
      721 gtgccgtgcg caagtggggc ggagttcctg cttactacac gcatgtcatg cgtgcgcacc
      781 atacgttcct ggcataccgt gctgaaggca gaacaccgcg gctcgagatt gcacagctgg
      841 ttgaggactg acctcagctc acacccgtag cgtcttgtcg aacacccaga actggcggtc
      901 attgcgccgc cagtcacgtg ctgggttgcg cacgaaattc atgcgcagat acagccgatg
      961 tgcagcatgc atcgtcggct gtgtagacag cacggccgag gagagtccgc gttcgactgc
     1021 gcgtgtctcg aacgcccggc ataaggcttc acctactcca cgggtgcgtg aggccgggtg
     1081 cgtcgcaagc aactgcatct ccgcttcacc cgatccggca acctgcctgt agggattgct
     1141 gtccgtggcc acgatgatca tgccgatcac gttcgtctcg tcgctcagcg cgagcagaac
     1201 ctcgccgcgc ttcttaactt cgtcaggctg aaacatgcgt gccgcaattt ccggagctgt
     1261 gaaacccgcg tcgacatatg cagcctgcag aagacctaca atcgcggtgt cgtgctgctg
     1321 ttcttcgggt cggtagcgga tgatggtcgg caagtgtcaa ccccggcaga ttagacagtg
     1381 ggcaacttgc tcgcgtgcac cttgtctgcg agcgctgtcc tgtattgagc gagtttttgt
     1441 gcgagcacct catcgctgag cgcgagaatc tgcgcggcga gcaggccggc gttctttgca
     1501 ttttcgagcg cgaccgtagc aacgggaatt ccgccgggca tctgcaagat agagagaatg
     1561 ctatcccaac catcgagcga gttggaggat tttaccggta cgccaatcac gggcagagtg
     1621 gtcagcgaag caaccatacc cggaaggtgt gcggcgccgc ccgcaccggc gatgatgacg
     1681 tggtagccgt ttttctttgc ctctttggca aaaccgaaca ttttttcggg cgtgcggtgc
     1741 gccgagacca cctcggcatg cgcggcaaca ccgagctctt ttagaacttc ctgcgcggcc
     1801 tgcatgacct tccagtctga ttcagacccc atgatgatcg caactttggc cgccatacga
     1861 gattgactca ggggagcgcg ctgccgcgta tagtgcaaat tggtttagcc tggctcaagc
     1921 cccaaaggtc agcgtctcgt actgccgaat cagagtattg cgctcaccga caagtgtgat
     1981 atggcccagc ttgcggtatg gcctcatatc gcgcttgcca tagaggtgca cctgcgcgtt
     2041 tggcattttg agggcagtct gtatcattgc attccactgc gcgaccctgt cgctttcggg
     2101 accgagaagg ttaatcatca gcgagggcgc agtagtttcg ggtgaaccca gtggcagacc
     2161 agtcagtatg cgcaccaggt tttcgtattg gctggttgcc gccgcttcaa tcgtgtggtg
     2221 accactgttg tgcggacgcg gcgccatttc gttaatgagt atttcgcccc tttctgtcag
     2281 gaacatctct acggcaatga gcccgacaat gccgagagat
//
LOCUS       DAOQJF010000451         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_34803_length_2319_cov_4.327739, whole genome shotgun sequence.
ACCESSION   DAOQJF010000451 DAOQJF010000000
VERSION     DAOQJF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34803_length_2319_cov_4.327739"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLF85_16430"
     CDS             <1..653
                     /locus_tag="PLF85_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21308.1"
                     /translation="VLNFFDSELKGLVPYHRIFLATGESTDIRLRHLVKNPRIIYYTP
                     EPPPNFNAKQYFPQDLYLEEIHKSDLKVPANLKTEVTIPILYKTRLPIGYIQTNTPRA
                     IDDILMQTIKKIGVALETQLRKVGLQFEEPRPLPISNIDMHRMELEITDRLLLRHFQP
                     GFVVLFRIQKQQESLGQFSAVVSKSNNLGGGKTQIVLDFQDLDAMSELNLEEALKG"
     gene            complement(700..1062)
                     /locus_tag="PLF85_16435"
     CDS             complement(700..1062)
                     /locus_tag="PLF85_16435"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNE21309.1"
                     /translation="MGNQITAKARRLRKQPTIAEMILWQSLRRQGLGFRFRRQQPFRI
                     KLGDYTRVFIADFYCSQKKLAIEVDGKIHEAQADYDKVRDQLISCLGVTVLRFSNTTI
                     EQDLPIVLSNIRTALSRL"
     gene            complement(1118..1888)
                     /locus_tag="PLF85_16440"
     CDS             complement(1118..1888)
                     /locus_tag="PLF85_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21310.1"
                     /translation="MFFKSKPKKEVYTPENKAEVLHKLREKYRRANGEHGARFFDLNK
                     LEERITFQRATQSDFQRFILDEMQFFKAMEEKALEELARRKRQEEADARMREIMEAND
                     ALIAHYPDTFFHPMATLECRRIVGAVSNLFPEIDNELRYVFQGRKEWGEIRFYLSDLE
                     RFLYKPPMRMTAFLAAYVAEIQKWGEAGRDDADRKFLQTAAMCLTGISNEIGAALEIL
                     ADDDKRRASAIKARVEGIVSDFRLTDWAQHGLALKQKK"
     gene            1990..>2319
                     /locus_tag="PLF85_16445"
     CDS             1990..>2319
                     /locus_tag="PLF85_16445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE21311.1"
                     /translation="MLENTPSRTAEVVALIRALEYAYYGEKSLLKDKYAARFLSDFYM
                     AIYNRLSILPEFMSFFVNSASLGLFDFITLRHAFMDDYTRRLAQKIPVAILGAGYDSR
                     ALRLAHHI"
BASE COUNT          508 a          669 c          600 g          542 t
ORIGIN      
        1 ttgtgctgaa cttcttcgac agtgaactca agggcctggt gccttaccac cgcatttttc
       61 tcgcaaccgg cgaatcaacc gacatccggt tacgccatct cgtcaagaac ccgcgcatta
      121 tttactacac accagaacca ccccccaact tcaatgcgaa gcagtacttt ccgcaagacc
      181 tgtatctcga agaaattcat aagagcgacc tgaaggtacc ggcaaacctc aaaaccgagg
      241 tgacgattcc gattctttac aagacccgcc tgccgattgg gtatatacaa acaaacacgc
      301 cgcgcgctat tgatgatatt ctgatgcaga ctatcaaaaa gatcggtgtg gcactcgaga
      361 ctcagctgcg caaggtggga ttgcagtttg aagagccccg ccccctgccg atcagcaata
      421 ttgacatgca ccggatggag ctcgagatca ccgaccgcct gttgctccgc cactttcagc
      481 cgggctttgt ggtacttttc cgcatacaga agcagcagga gagcctgggc cagttttccg
      541 cggtggtcag taagagcaac aatctcggtg gcggcaagac gcagatcgtg ctcgactttc
      601 aggatctcga cgcgatgtca gagctgaatc tcgaagaagc attaaagggg tagcgcccct
      661 atcccccacc cctttcccca agtttgggga aaggggattt cagagtctac ttaaggccgt
      721 tcgtatattg gatagaacga taggtagatc ctgctcaatg gtcgtattgc tgaatcgcaa
      781 tacggtcaca cccaaacacg atatcaactg gtctcggacc ttgtcgtagt ccgcctgtgc
      841 ttcatggatc ttgccatcaa cctcaatcgc cagttttttc tggctacagt aaaaatcggc
      901 tataaaaacc cttgtgtaat caccgagctt tatgcgaaac ggttgctggc gcctgaagcg
      961 aaatcccaga ccctggcggc ggagactctg ccagagaatc atctctgcaa tcgtcggttg
     1021 ctttctgagt ctccgagcct tagccgtaat ctggttaccc atgccaaatc tcctgttccc
     1081 acctccccca gacttggggc aggccgggag ggggcgctta ttttttctgt ttcagcgcta
     1141 aaccgtgctg cgcccagtcg gtcaggcgaa aatcactgac gataccttcg acgcgggctt
     1201 taatggccga ggcacggcgc ttatcatcat ccgcaagaat ttcgagcgcg gcgccgattt
     1261 cgttgctgat acccgtcagg cacattgcgg cagtctgcag aaacttgcgg tcagcatcgt
     1321 cacggcccgc ttcgccccac ttttgtatct cggccacgta ggcggccaaa aacgcggtca
     1381 tgcgcatcgg gggcttgtag agaaaacgtt ctaaatccga caggtaaaac ctgatctcgc
     1441 cccactcttt gcgcccctgg aaaacgtagc gcagctcgtt gtcaatttcg ggaaaaaggt
     1501 tcgatactgc gccgacgatg cggcggcact cgagagtcgc catcgggtgg aagaaggtgt
     1561 cggggtagtg cgcaataagc gcatcgttgg cttccatgat ttcgcgcatg cgcgcgtcgg
     1621 cctcttcctg ccgcttgcgg cgtgccagtt cttcgagcgc cttttcttcc atcgccttga
     1681 agaactgcat ttcatcgaga atgaaacgct gaaaatcgga ctgcgttgcc cgctggaacg
     1741 tgatgcgttc ttcaagcttg ttgagatcaa agaagcgcgc accgtgttcg ccgtttgcgc
     1801 gccggtattt ttcgcgcagc ttgtgcagaa cctcggcctt gttctcgggg gtgtagactt
     1861 ctttcttagg ttttgactta aagaacataa catgtccccc tcccggcctc ccccgcatgc
     1921 gggggaggtg ccagaggcgg agggggataa tttacttgtc cctaccaatt atcggcaaaa
     1981 aacctaccca tgttagagaa tacgccgagc agaacggccg aggtcgtggc gctgattcgc
     2041 gccctcgagt acgcttatta cggcgagaag agcctgctga aagacaagta cgccgcgcgt
     2101 tttctctctg acttctacat ggcgatctac aaccggcttt ccattctgcc ggagttcatg
     2161 tcgttcttcg tcaacagcgc gagcctcggc cttttcgact ttatcacgct gcggcacgcc
     2221 ttcatggacg actatacccg tcgcctggcg cagaagattc ctgtggcgat cctgggcgcg
     2281 ggctacgatt cacgcgcgct gcgcctggcg caccacatc
//
LOCUS       DAOQJF010000452         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_34804_length_2319_cov_4.313604, whole genome shotgun sequence.
ACCESSION   DAOQJF010000452 DAOQJF010000000
VERSION     DAOQJF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34804_length_2319_cov_4.313604"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="PLF85_16450"
     CDS             <1..2157
                     /locus_tag="PLF85_16450"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNE21312.1"
                     /translation="IRLAVPITAGCFTLFILSLLVMQAIPASVADAQQALAQLKARQT
                     DIVIDRNGRELNRLGVAADHKASLTEFAKWQIDALLFSEDRNFYSHHGVSYKAIARAA
                     LNNTFRLRYAQGASTITQQLARILLQNRQKSIWRKLQEIRLARALESILPKQKILELY
                     VNHVYLGHGNYSFSSAAKFYYDKPVAGLTPAEFLSLVALIPSPEKYSPLRNNRLLQGR
                     MQVLWQAMESTGIAPVTRQEYLAGLRSVSAQGERFATETAFGEKSRSGLWPALYARDF
                     LMQRRLLSGENQNSARVFTTIDTDLQQRAEELVVRHLRAARRNFRGVLKSADAKDQRL
                     LQKLRSASHDYGLLLDLAGLPVRNEALDELQAALIALNPRTGEILAMVGGENFESKNQ
                     LNRTIQMRRQTGSAIKPFIYAKAISQHLVHPATLVDDTPYIVGSGAKTWAPENINGSF
                     EGPIPVRDALAKSRNIPAIRVGRLLGRQSVTELFGDFFFQTEQALESRFAYDETVAIG
                     TISLSPLEMARAFSVFANGGFLNDPLLVTRVETPEKVFDLRKTHADQMGLAPAGKERL
                     LSAAESQLMVSLLKSSGRNAGTGVGGVIGKTGTSSESRDLWFIGGGKEIIVAVWFGYD
                     DMRISIPGATGSALAARLAGDFLKTDFAPVEFKMQPGMVRLRVCPLSGRLASESCRHS
                     RSEVFLAGVIPEGECLHGIAEEQSEFMAVMGDSQFR"
     gene            2138..>2319
                     /locus_tag="PLF85_16455"
     CDS             2138..>2319
                     /locus_tag="PLF85_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21313.1"
                     /translation="MIRSSVSFLLLLVIFLICGSAEARTSDYRRAQTLAKTDPLEAEA
                     LFERFMRETPNGKVRRA"
BASE COUNT          478 a          665 c          675 g          501 t
ORIGIN      
        1 atccgtttgg ccgtcccgat caccgcgggt tgttttactt tgtttatcct gtcgctgctg
       61 gtaatgcagg cgattcccgc ctctgtggcc gacgcacaac aggctctcgc gcagctgaag
      121 gcccggcaaa cggacattgt catcgaccgc aacggtcgcg agctcaacag actgggcgtc
      181 gctgccgacc ataaggctag cctgactgag tttgcaaagt ggcagatcga cgctctgctc
      241 ttttcagaag accgcaattt ctatagccac cacggcgtct cttacaaggc gattgcgcgt
      301 gctgcactga acaatacttt tcggctgcgc tacgcacagg gagcttctac cattacgcag
      361 cagcttgcgc gtatattgct gcagaaccgg cagaagagta tctggcgcaa gctgcaggaa
      421 atacgcctcg cgcgcgcact cgagagcata ttgccgaagc agaagatact cgagctctac
      481 gttaatcatg tctaccttgg tcacggcaat tattctttct cttcagccgc aaagttctac
      541 tatgataagc cggtggctgg cttaacgccc gcggagtttc tgtcgcttgt cgcgctgatt
      601 ccatcgcccg agaaatattc accgctgcgg aacaaccggc tgctgcaggg ccgcatgcag
      661 gtgttgtggc aggcgatgga aagcactggc attgctcctg ttacgaggca ggaatacctt
      721 gcaggattgc gcagcgtttc ggcacagggc gagcgttttg cgactgaaac ggcgttcggc
      781 gaaaaatcgc gatcgggatt atggcctgcg ctgtatgcgc gcgattttct gatgcagcgg
      841 cgcctgcttt ccggcgaaaa ccagaactcc gcccgtgtct tcaccacgat agatacagat
      901 ctgcagcaaa gggccgaaga gcttgtcgtg cgccacctca gggcagcacg ccgcaatttt
      961 cgcggagttc tcaaatcggc cgacgccaag gaccagcggt tgctgcagaa actgcgcagc
     1021 gcatcgcacg attacggtct gctgctggat ctcgcggggc tgccggttag aaacgaagca
     1081 ctcgatgagc tgcaggcggc tcttattgcg ctcaatccgc gtaccggaga aattctcgcg
     1141 atggtaggcg gcgagaactt cgagagcaaa aaccaactga atcgtaccat acagatgcgc
     1201 cggcagacgg ggtcggctat taaacccttc atctatgcca aagccatcag ccagcacctc
     1261 gtacacccgg cgacgctggt cgacgatacg ccttatatag tcggttcggg tgcaaaaacc
     1321 tgggctcctg aaaatatcaa tggcagtttc gaggggccca tacccgtgcg cgacgcgctg
     1381 gcaaaatcac ggaatattcc cgcaattcgg gtcggcaggc tgctcggcag gcaaagcgtc
     1441 acggagcttt tcggcgattt cttcttccag accgaacagg cgctggaaag ccgctttgct
     1501 tacgacgaaa cggtcgcgat tggcacgatc tccctctcgc cgctcgagat ggcgcgcgcg
     1561 ttttctgtgt tcgccaacgg tggtttcctg aatgacccgc tgctcgtaac gcgggttgag
     1621 actcccgaaa aggtatttga cctgcgcaag acccacgccg accagatggg gcttgcgcct
     1681 gccggcaaag agcgcctgct atcggcagcc gagtcgcagc tgatggtttc gctgctcaag
     1741 agttccggca ggaatgccgg cacgggggtg gggggagtga tcggcaaaac aggtacctca
     1801 tccgagtcgc gtgacctctg gttcattggc ggcggaaaag aaattatcgt cgcggtctgg
     1861 tttggctacg atgatatgcg tatttcgatt ccgggggcca ccgggtctgc gctcgccgcg
     1921 cgccttgccg gcgactttct gaaaaccgac tttgcgccgg ttgaattcaa aatgcaacct
     1981 ggcatggtgc ggctaagagt ttgcccgtta tccggcaggc tcgcatcgga atcttgccgc
     2041 cattcacgca gcgaagtctt tctcgccggc gtgattcccg agggcgagtg cctgcacggt
     2101 atagccgaag agcagtcgga attcatggcg gttatgggtg attcgcagtt ccgttagctt
     2161 tttgctcctg ctggtaatct tccttatttg cggcagcgcc gaagcccgca cgtccgatta
     2221 ccggcgcgcg cagaccctgg ccaaaactga cccccttgaa gctgaagcac tctttgaacg
     2281 attcatgcgt gagacgccga acggcaaggt gcggcgcgc
//
LOCUS       DAOQJF010000453         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_34893_length_2316_cov_4.558160, whole genome shotgun sequence.
ACCESSION   DAOQJF010000453 DAOQJF010000000
VERSION     DAOQJF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34893_length_2316_cov_4.558160"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PLF85_16460"
     CDS             complement(<1..1156)
                     /locus_tag="PLF85_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNE21314.1"
                     /translation="MDILQVALFAVAFFTSFAYAGYAVMKYWRLAHLGQAQEIKSTFG
                     QKVKNIIINVFLQKKLMKRPVRGIFHIFIFYGFLVYGGHTTSQFIGGFFGPYQMPVVG
                     GTGYDFYIPKLIDGIIPHFHYVYDYLLDIFSILVLSGLAFFALRRWVYRAYELDRPSG
                     QSMIVISMISTLMISTLVGEGANAIRLGTAADHISPIRQAMVALLSGAGLTSANAGYW
                     YIGGWWAHILTVFAFMAFVPNSKHAHLIWAPVNFWFEKETPKGQLSFLDTENAKVWGA
                     ANVQDFTWKDHLNGLSCIECGRCTIECPANRTGKQLNPKNIMGDLKHALMEQMNKVEA
                     AKKAGKSDEEIAAMPELRVIDNYTSQESLWACTTCYACVEACPVGNNQVDA"
     gene            complement(1243..>2316)
                     /locus_tag="PLF85_16465"
     CDS             complement(1243..>2316)
                     /locus_tag="PLF85_16465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE21315.1"
                     /translation="LPVIDLLRKSSDEAEKARYFEILNTSGQLLLRVVNDILDLSRIE
                     RSALDLEEETVDLRSVAATSMELYQAKATAKKLAFHLHVDPALHTAYLTDRTRLTQVL
                     NNLVSNALKFTQQGSVDLELRLESDQPDSSVVLFVIRDTGIGVPPNKQAAIFENFEQA
                     DRSIALRFGGSGLGLPISKALVELMGGSISLRSPISENAQLPGTEFIVRLPMRKTAAP
                     YQAAAATQPFTAGQFTGLRALIVEDNPVNRLVLGKTLEKLGVSYDEAVDGLAALQLAQ
                     KQTYNIIFMDLEMPEIDGYETAKRLRSAPGPNQQTHVICITAHTLAEQRERVLAAGMN
                     EMVRKPFSLQEIASAVANARPAG"
BASE COUNT          506 a          602 c          688 g          520 t
ORIGIN      
        1 ttgcatcgac ctggttgttg cctaccgggc aggcttcgac gcaggcgtag caggtcgtgc
       61 aggcccagag tgattcctgt gaggtgtagt tgtcgatcac gcggagctct ggcatagccg
      121 cgatttcttc atcgctcttg ccggcttttt tcgccgcttc aaccttgttc atctgctcca
      181 tcagcgcatg cttcaggtcg cccatgatgt tcttaggatt gagttgtttg ccagtacggt
      241 ttgcagggca ctctatcgtg cagcgaccgc actcgatgca gctgaggccg ttcaggtgat
      301 ctttccaggt gaaatcctgt acgttagcag caccccagac cttcgcattt tcggtgtcga
      361 gaaatgacag ctggcctttg ggagtttctt tttcaaacca gaagttaacc ggcgcccaga
      421 tgaggtgcgc gtgctttgaa ttcggtacaa acgccatgaa ggcaaagacg gtgagaatgt
      481 gtgcccacca gccgccgata taccagtaac ccgcgttggc agaagtaaga cctgcgcccg
      541 agagcagggc aaccatcgcc tggcgaatcg gtgaaatatg gtctgcggcg gtgccgaggc
      601 gtatcgcatt agctccctcg ccgaccaggg ttgagatcat cagcgtcgag atcatgctga
      661 taacgatcat tgactggcct gaagggcggt cgagttcgta cgcacggtat acccagcggc
      721 gcagcgcaaa gaacgcaagg cctgaaagaa ccaggatcga gaagatgtcg agcaggtagt
      781 cgtacacgta gtggaagtgg ggaatgatac cgtcgatcag cttcggaatg tagaagtcat
      841 aaccagtgcc gccgacaacc ggcatctggt atggaccaaa aaagccgccg atgaactggc
      901 tggtggtgtg accgccgtag acgaggaagc cgtaaaagat gaagatgtga aagataccgc
      961 gcaccgggcg cttcatcagc ttcttctgca ggaagacatt gatgatgata ttcttgacct
     1021 tctggccgaa ggtcgatttg atttcctgtg cctggccaag gtgcgcaagt cgccagtact
     1081 tcatgaccgc gtagccggcg taggcaaacg aggtaaaaaa tgccactgca aagagggcaa
     1141 cctgtaagat atccataagg aaatatcggg aaaatcaggg ggagtgtaaa gagaaatcag
     1201 gcaggttttt acctcggagg agtaaatacc acactcgggc ggtcagccgg ctggccgcgc
     1261 atttgccact gcactggcga tttcctggag cgaaaagggt ttgcgcacca tttcattcat
     1321 gcccgctgcc agaacgcgtt cgcgctgctc ggccagggtg tgagcggtaa tgcagataac
     1381 gtgtgtctgc tggttgggcc cgggtgcaga acgcaggcgt ttcgccgttt catagccgtc
     1441 aatttcaggc atttcgaggt ccatgaagat gatgttgtat gtctgcttct gcgccagctg
     1501 cagggcagca agaccgtcca ctgcctcgtc gtaagaaaca cccagtttct ccagggtttt
     1561 gcccagcacc agccggttga ccgggttgtc ctcgacgatc agggcgcgca atccggtaaa
     1621 ttgccctgcg gtaaatggtt gcgttgcagc agcggcctgg tagggggcag ctgtcttgcg
     1681 catgggcagg cggacgataa actcggttcc ggggagctgc gcgttttcag agatcgggct
     1741 cctgaggctg atgctgccgc ccataagttc tacgagtgcc ttgctgatcg gtagtcccag
     1801 cccgctgccg ccaaagcgga gcgcgatcga acggtcggcc tgttcgaaat tctcaaaaat
     1861 tgctgcctgc ttattgggcg gcacgccgat tccagtatca cgaatgacaa acagcacaac
     1921 cgacgaatca ggttggtcag attccagacg caattccagg tcaacgctcc cttgctgggt
     1981 aaatttgagc gcattactca ccaggttatt cagtacctgc gtgagcctgg tacgatcggt
     2041 caggtatgcg gtatgcagcg ccgggtcaac atgcaggtgg aatgccagtt tctttgccgt
     2101 ggcctttgcc tggtagagtt ccattgaggt agcggcgaca gaccgcaggt cgacggtctc
     2161 ttcttccaga tcgagagcgc tgcgctctat acgcgagaga tcgagaatgt cgttcacgac
     2221 ccgcagcagt aactggccag aggtgttgag tatttcaaag tagcgcgcct tctctgcttc
     2281 gtcgctgctc ttgcgtagca ggtcgatcac cggcag
//
LOCUS       DAOQJF010000454         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_35157_length_2307_cov_2.886767, whole genome shotgun sequence.
ACCESSION   DAOQJF010000454 DAOQJF010000000
VERSION     DAOQJF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35157_length_2307_cov_2.886767"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLF85_16470"
     CDS             complement(<1..1216)
                     /locus_tag="PLF85_16470"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNE21316.1"
                     /translation="MPAGNAWYAANRETFLNESTETIAAKLLGAAAEDGWKVEPEQHE
                     EWKSSIFILKNELNAASSKVEIIKEAIRQLEPEFIHGVVLEYDFRRRGLRIDCILFAT
                     GTIFILEFKRSRLQAADTDQIMQYCVSLYEFHAQTRRLVDELKFNIVPILVLTEGHLT
                     AKENGLSFFSENWPRILRKPMKSDHENLHTALITGQKMRQTDQTANLQKWLESEFSPS
                     STILDAALSLYGNHDVSAIKDHQTPLALMKKCTAAILEEIDQALKEKRNHLIIISGSP
                     GSGKTLVGLDIAFSQRYRQDAVFVTGNAPLVEVLQSALQLSYRGMNNRTRTNLTGYAK
                     ENFRYVGTSTTFKIVKAHNFLGQRGEGHRSSDGRILVFDEAQRTYEKGRVVLREKLEE
                     HEAELIMQAQEKL"
     gene            complement(1241..1537)
                     /locus_tag="PLF85_16475"
     CDS             complement(1241..1537)
                     /locus_tag="PLF85_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21317.1"
                     /translation="MNIKMTYLYRDGGNYKTWQEVVFTNHSGKTAEQLTDEIKQRLIS
                     GQFFDQGRAPIPFEYPDSYDPELDHTWLEFDSLEEVDDRPDVPQDISDFIFSLK"
     gene            complement(1567..>2307)
                     /locus_tag="PLF85_16480"
     CDS             complement(1567..>2307)
                     /locus_tag="PLF85_16480"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HNE21318.1"
                     /translation="DGIEIHVTLSSGIAVAPNDGTDPDELIRYADSAMYHAKERGRNT
                     FHFFDARISDSAKSRFVLETATAQAQQWQKLTATQFQMSVNISPRQFRQGKIIADLKE
                     LLARYALEPGRIKLEVTEGLLLHPYDNPVEVLNQIKSMGVGVAMDDFGTGYSSLLNLR
                     QFSFDLLKIDRSFIHDLTETAQTRAMVNAIIAMCKSLGLQVIAEGIETTAQRDYLLQQ
                     GCTLGQGYLFAKPVPGAEFQRLLGSSFV"
BASE COUNT          523 a          576 c          594 g          614 t
ORIGIN      
        1 agagcttttc ttgagcttgc ataattagct ctgcctcatg ttcctccaat ttttcgcgca
       61 aaacgacgcg acctttttcg tatgtcctct gagcctcgtc aaagactaag atgcggccat
      121 cactagatct atggccttcg ccgcgctgcc ccagaaaatt atgggcctta acgatcttga
      181 atgtcgtact ggtcccgaca tagcgaaagt tctcttttgc gtaacctgtt agatttgttc
      241 gggtacggtt attcattcca cgataggaaa gttgcagggc gctctgcaga acttccacta
      301 atggagcatt ccccgtaaca aaaaccgcat cctgacggta tcgttgactg aatgcgatgt
      361 ccaagccgac gagtgttttc ccagaaccgg gagagcctga aattattatc agatgatttc
      421 ttttctcttt aagagcttgg tcaatttcct ccagaatcgc cgccgtacac ttcttcatga
      481 gggcaagcgg agtctgatgg tcctttattg cagacacatc gtgattgccg taaagagaga
      541 gtgcagcgtc caatatcgtt gaggagggtg aaaactcaga ttctagccat ttctgtaaat
      601 ttgcggtttg atcggtttgg cgcatttttt ggccggtgat tagggctgta tgaagatttt
      661 catgatcgct tttcatcggc ttgcgtagaa ttctcggcca attttcagaa aagaacgaaa
      721 ggccattttc tttagcagtt aagtggccct ctgtgagaac caaaataggt acaatgttaa
      781 attttaattc gtccactaag cgacgggttt gcgcatgaaa ctcatagagg ctcacgcaat
      841 attgcatgat ttggtctgtg tcggcagctt gcaggcgcga gcgcttgaat tccagaataa
      901 atatagtccc tgtcgcaaag agaatacaat cgattcgcaa gccgcgtcga cgaaaatcgt
      961 attccagaac cacgccgtgg atgaattctg gctccaattg gcgaatggcc tctttgatga
     1021 tttcaacttt gctactagca gcgttcagct catttttgag gataaagatg ctacttttcc
     1081 actcttcgtg ttgctctggc tcgactttcc atccatcttc agcagctgcg ccaagtagct
     1141 ttgcagcaat ggtttccgtc gattcattta ggaaagtctc gcgatttgca gcataccaag
     1201 cgttgccagc gggcattgtg ctccctatag atttagaacc tcacttcaag gaaaaaatga
     1261 aatctgaaat gtcttgcgga acatctggtc tatcgtcaac ctcctcaagg ctatcaaatt
     1321 ccagccaagt atggtcgagt tctggatcgt acgagtcagg gtattcaaaa ggaattggtg
     1381 cgcggccctg atcgaaaaat tggccagaaa tcaggcgctg cttgatctcg tcggtgagtt
     1441 gctccgcggt ttttccggag tgatttgtga agactacttc ctgccaagtc ttgtaattac
     1501 ctccatcccg gtagaggtac gtcatcttga tgttcatcac ctggctgaat gcgtcggtgc
     1561 agcctgtcaa acaaaagagc tgcccagtaa ccgctgaaac tccgccccgg gcactggttt
     1621 tgcaaacagg tagccctggc ccagggtaca accctgttgc aagaggtagt cgcgctgcgc
     1681 cgtggtctcg ataccttcgg cgatcacctg caggccgaga ctcttgcaca tcgcgatgat
     1741 tgcgttcacc atcgcgcgcg tctgcgcggt ttcagtcagg tcgtgaatga acgagcggtc
     1801 gatcttgagc aggtcgaaag aaaactggcg gaggttcagc agcgacgaat aaccggtacc
     1861 aaaatcatcc attgcgacac cgacacccat actcttgatc tggttgagca cttcgaccgg
     1921 gttgtcatac gggtgcagca gcaggccttc ggtgacctcg agcttaatgc ggccgggttc
     1981 gagcgcgtaa cgcgcgagta gttctttcag gtcggcgatg atcttgccct gccgaaactg
     2041 gcgcggcgag atgttgacgc tcatctggaa ctgcgtggcg gtaagcttct gccactgctg
     2101 cgcctgcgcg gtggccgttt cgagcacaaa gcggcttttg gctgagtcgc tgatgcgcgc
     2161 gtcgaaaaag tggaacgtat tgcggccgcg ctctttcgca tggtacatcg ccgagtcggc
     2221 ataccgtatc agctcgtcgg ggtctgtgcc gtcgttggga gcaaccgcga taccgctgct
     2281 gagcgtgacg tgtatttcaa tgccgtc
//
LOCUS       DAOQJF010000455         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_36214_length_2271_cov_4.972924, whole genome shotgun sequence.
ACCESSION   DAOQJF010000455 DAOQJF010000000
VERSION     DAOQJF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36214_length_2271_cov_4.972924"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..915)
                     /locus_tag="PLF85_16485"
     CDS             complement(391..915)
                     /locus_tag="PLF85_16485"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNE21319.1"
                     /translation="MLPASVREIAVTPMGFAVIVKPEFKEKIIPIFIGPSEAYAISSI
                     LLNEKTERPVGADLLRSVIEATGASVNKVFINDFHGGTFFARVYITGTHFEGGLLELD
                     ARPSDAIALAVRFASPVYVAEHVYDRTAIDPTTLREADSDALRTGESNVDDILTADER
                     EEFFEAISYAFDSG"
     gene            1012..>2271
                     /gene="hemG"
                     /locus_tag="PLF85_16490"
     CDS             1012..>2271
                     /gene="hemG"
                     /locus_tag="PLF85_16490"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNE21320.1"
                     /translation="MHRIAVVGGGISGLCHAWYIKKTLPDSEVTIFEGGKWGGNIATE
                     VHEGCTLEPGPDSYLDRNGVFNSLAAELGIADKLLFEQKRSAKRFILKEGYLVPAPKS
                     PLGLITTSLLFFPEKIRIFLAMGKKFSLWPTITLFDFARNVLGFSAAEYMASPFARGV
                     YGSEAEDLEFSSMFPQLYEKISKAPKLKVAMKEYARERREFWQQELGDIKFERGIYSM
                     QGGLITLVNALQQNLEKAGVKFVPDKIARIQKGSSGKPVLSSKAKSYDTFDRVVFTGN
                     TGDLAYIFREHDKELSRRLGELKYSPINVVYSCWNKKEFSSSGYGFLVPRKERAPILG
                     SIFASNVFPGRAPEDRFLTKTMVSGDSELFKDEELASLAQESLGRILKSRATPLWSKV
                     YRHRPGIPRYAPGYGEWKREVLGLAAQH"
BASE COUNT          556 a          574 c          574 g          567 t
ORIGIN      
        1 catttattta tcatgtctgg atgattaata aaagaataga cctgcctatc tgattgtttc
       61 tggccaggtc catccattta taatcttcac catcaataaa taatagatac ttttatataa
      121 tgtcactacc accacctctt gcagccaaga tcaagtccat tccaatggat ccacttgctc
      181 ttgccaataa cgtggagtta gcatacatgc tttctttttc gaactatcgt aaattttatc
      241 tcatatttag agctatctcc agattctgta caatgcagtg ccaaagattc atacaactct
      301 cctctcgaat catataattt tgtcgatttt gatgattcca cctatgaaac actctatgca
      361 gaaacaaaga aaaactatta gtacaaaaac tcaaccactg tcaaaagcat aagaaatagc
      421 ttcaaagaat tcttcccgtt catctgcagt gagaatatca tcgacgttcg attcgccggt
      481 gcgcagcgca tcgctgtcgg cttcgcgcag cgtcgtcggg tcgatcgccg tgcggtcgta
      541 gacatgctct gccacgtaaa ccggtgaggc aaagcgcacc gcgagtgcga tagcgtcaga
      601 ggggcgcgca tcgagttcga gcaggccacc ttcgaagtgg gtgccggtaa tgtagacgcg
      661 tgcgaagaag gtgccgccgt ggaaatcgtt gatgaagacc ttgttgactg aagcccctgt
      721 tgcctcgata actgaacgca gcagatcggc gcccacgggg cgttcggttt tttcattcag
      781 gagtatggat gagatcgcat acgcttcaga cggcccgatg aagattggga tgatcttttc
      841 cttaaattca ggtttgacga taaccgcgaa ccccatcggg gttacggcaa tctcgcgtac
      901 gcttgcgggc agcatatttg aaccccgcaa ttgtcttcat ttctgtaaag ctatttttga
      961 cctttggtgt gattttaatt gccacgctgt gcaagtaaaa gaggcgaacg tatgcaccga
     1021 attgcggttg tcggcggggg aatctcgggt ctctgccacg cctggtatat caaaaagact
     1081 ttgcctgata gcgaagtgac gattttcgag ggaggaaaat ggggtggtaa tatcgccacc
     1141 gaggtgcatg aaggctgcac tcttgagcct ggccccgatt cgtaccttga tcgtaacggt
     1201 gtctttaaca gcctggctgc cgaactcggt atcgccgaca agctgctctt cgagcaaaag
     1261 cgcagcgcga aacgttttat cctgaaagaa ggttatctcg tcccagcgcc caaatcgccg
     1321 cttgggctga ttaccacgtc gctgcttttt ttccctgaga agattcgtat cttcctggcg
     1381 atgggcaaga agttctctct ttggccgact attactctct ttgacttcgc gcgcaatgtc
     1441 cttgggttca gcgctgcgga atacatggct tctccctttg cacggggcgt ttatggcagc
     1501 gaggccgaag atctcgaatt ttcttcgatg ttcccgcagc tttacgaaaa aatcagcaaa
     1561 gctcccaagc tcaaggttgc gatgaaagaa tacgcacgtg aacggcgtga gttctggcag
     1621 caagaactgg gcgatataaa gtttgaaagg ggtatctaca gcatgcaggg cgggttgatt
     1681 acgctggtta acgcgctgca gcagaacctc gaaaaagccg gagtaaaatt tgtaccagac
     1741 aagattgcgc gcatccagaa gggcagcagc ggcaagccgg tactttcctc taaggcgaag
     1801 tcgtatgata catttgaccg cgtggtcttt acgggtaaca ccggcgatct ggcctatatc
     1861 ttccgcgagc acgataaaga gctctcgcgc cgcctgggcg agctcaagta cagcccgatc
     1921 aacgtcgttt atagttgctg gaataagaaa gaattctcca gctctggtta cggctttctc
     1981 gtgccccgca aagaacgtgc tccgatacta ggcagcatat ttgcgtcgaa cgtctttcct
     2041 ggcagggcac ccgaagatcg tttcctgaca aagaccatgg tctccggcga cagcgaactc
     2101 ttcaaagacg aagagctagc atctcttgca caagagtcgc tcggccgcat cctgaagagc
     2161 agggcgactc cgctctggag caaagtttac cgccaccgtc ccggcattcc gcgttatgcg
     2221 cccggctacg gcgagtggaa gcgcgaggtg ctgggtctcg cggcgcagca c
//
LOCUS       DAOQJF010000456         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_37083_length_2242_cov_4.289895, whole genome shotgun sequence.
ACCESSION   DAOQJF010000456 DAOQJF010000000
VERSION     DAOQJF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37083_length_2242_cov_4.289895"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLF85_16495"
     CDS             <1..324
                     /locus_tag="PLF85_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE21321.1"
                     /translation="FITHDLGIVARLAHRVAVMYAGEIVETGTVAEIFAAPKHPYTRA
                     LLASVADVPRGSRLSAIPGSPPQLSEAIHGCAFAPRCSFAEPACRQTIPLRAAEGHEW
                     RCIHE"
     gene            317..784
                     /locus_tag="PLF85_16500"
     CDS             317..784
                     /locus_tag="PLF85_16500"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNE21322.1"
                     /translation="MSKVPVSVYCDGASRGNPGPAAYGVAAFRKGEENSLSAFRAGED
                     STVFKLAGKIGKHTNNEAEYYGIIRALEKCLELGLTGVVLHSDSELVIRQLRGEYKVK
                     NENLKPLFEQASSLAQRVKPVLVHVRREKNQIADYLANLALDSAGNSPFATDG"
     gene            777..>2242
                     /locus_tag="PLF85_16505"
     CDS             777..>2242
                     /locus_tag="PLF85_16505"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE21323.1"
                     /translation="MAEEESAEFAPGVLPPWCDASELSDLQGEAVEVAIEERSLKPGV
                     LNAICTQAFERIGSALPDYQLRDAQVQMAQQILRSFYRERSAVIEAATGVGKSFAYMV
                     AALAYNYLTGERVLIATETKALQWQLFEKDLPFLKRALDGSLRFELCLGSGNYYCRLR
                     YEQMMQEGSFRDIIDTEKLAEVRNWANLIATESDHSGSRFTDAPKLPEPIWSLITRDS
                     DGCPGNRCPHYTGCNYYRERKKWGDAQILVANHHLLLYHLLNDKHTLPDYGAVMIDEA
                     HGLVRTGYSIFTASFGRHEFADLKKGIDKTLVQHTGIQGEQKQEIEELVSLCLTRWET
                     FFSRWEVETDMVFKETGTALIRARVSDIPEITQPLVELSEKYESLKSDELEASLQGVI
                     NAQAKAIKKMAAFARAYDTPDSEAAVYWAEKRNDKFFLHSCRLNLGETLADLMPELRL
                     YTSATIGYWPYPEFPTRKSELIQRGFFNRFLSEALPLA"
BASE COUNT          513 a          691 c          619 g          419 t
ORIGIN      
        1 tttatcacgc acgacctcgg catcgtcgca cgcctcgcgc accgcgtcgc cgtgatgtac
       61 gctggcgaaa ttgtggaaac cgggacggtg gcggaaattt ttgccgcacc gaaacatccc
      121 tacaccaggg ccctgctcgc ttcggtagcc gacgtgccgc gcggcagccg tctaagcgca
      181 atacctggat cccccccgca gctcagcgag gcgatacatg gctgcgcatt tgccccacgc
      241 tgcagttttg cagaaccggc ctgccggcaa acaattccac tgcgcgctgc tgaaggtcat
      301 gagtggcgct gtatccatga gtaaagtgcc tgtatccgtt tactgtgacg gggcatcgcg
      361 gggcaacccc gggcccgctg cctacggcgt cgctgcgttc cgaaagggtg aagagaactc
      421 actcagcgcc ttccgcgcag gtgaagatag cactgtcttc aagctggcag ggaaaatcgg
      481 caagcacacc aacaacgaag cggaatacta cggcatcatc cgcgcgcttg aaaaatgcct
      541 cgagctgggc ctcaccggcg tggttctgca ttcagacagc gaactcgtca tcaggcagct
      601 gcgcggtgag tacaaagtca agaacgagaa tctgaaaccc ctctttgaac aggccagttc
      661 actggcgcag cgagtaaaac ctgttctggt acacgtgcgg cgcgagaaaa accagatcgc
      721 cgactacctg gcaaacctgg ccctcgacag cgcgggaaac tccccctttg ccaccgatgg
      781 ctgaagaaga atcggcagaa tttgcccccg gtgtattacc gccctggtgt gacgcctcgg
      841 agctcagcga cctgcagggt gaagcggttg aagttgccat tgaagagcgg tcgctcaaac
      901 caggggtgct caatgcgatc tgcacacagg cgttcgagcg tattggctcc gccctgcccg
      961 actaccagct gcgcgatgcg caggtacaga tggcgcagca gattctgcgc tccttctacc
     1021 gcgaacgcag tgcagtaatt gaagcagcca cgggtgttgg aaaatcgttc gcctacatgg
     1081 tggctgcact agcctacaac tacctgacag gcgaacgcgt cctcatcgcg acagaaacca
     1141 aggcgctgca atggcagctg tttgaaaaag atctcccttt ccttaagagg gcgctcgacg
     1201 gcagcctgcg ctttgaactt tgcctcggct cgggcaacta ctactgccgc ctgcgctacg
     1261 aacagatgat gcaggaaggc agcttcaggg acatcatcga caccgaaaaa ctcgccgagg
     1321 tgcgcaactg ggcgaacctg atcgcgaccg aaagtgacca ttcgggcagc cgcttcaccg
     1381 acgcccccaa attgcccgaa cctatctggt cgctgattac ccgcgacagc gacggctgcc
     1441 ccggcaaccg ctgtccgcac tataccggct gcaattatta ccgcgagcgc aagaaatggg
     1501 gcgatgcgca gatactcgtc gccaaccacc acctgctgct ctatcacctg ctcaatgaca
     1561 agcacacgct gccggactat ggcgccgtga tgatcgacga agcgcacggc cttgtgcgga
     1621 cgggttattc gatctttact gcgagttttg gccgccatga atttgccgac ctgaaaaagg
     1681 gcatagacaa aaccctggtg cagcataccg gaatccaggg cgaacaaaag caggaaatag
     1741 aagaactcgt ttcgctctgc ctgacgcgct gggagacctt tttttcccgc tgggaagtcg
     1801 agaccgacat ggttttcaaa gagaccggta ccgctctgat tcgtgcccgg gtgtcagata
     1861 ttcccgagat cacgcaaccg ctcgtcgaac tatcggagaa gtacgagagc ctgaaatccg
     1921 atgaactcga agcttcgctg cagggagtca tcaacgcaca ggcaaaggcg atcaagaaaa
     1981 tggcagcatt cgcccgtgct tacgacacgc ccgactctga ggctgcggtc tattgggcag
     2041 agaaacgtaa cgacaagttc ttcctgcact cctgccggct gaacctcggc gaaaccctcg
     2101 ccgacctgat gcccgaactg cgcctctata cctcggcgac catcggctac tggccgtacc
     2161 cggagttccc gactcgcaaa tcagaattga tccagcgcgg ctttttcaac cgctttctga
     2221 gcgaagccct gccgctcgcg ca
//
LOCUS       DAOQJF010000457         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_37327_length_2234_cov_4.941257, whole genome shotgun sequence.
ACCESSION   DAOQJF010000457 DAOQJF010000000
VERSION     DAOQJF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37327_length_2234_cov_4.941257"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1852)
                     /locus_tag="PLF85_16510"
     CDS             complement(62..1852)
                     /locus_tag="PLF85_16510"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNE21324.1"
                     /translation="MNHGRFARFADLLRLVFPLLLLLSLAITWKFLDASVAQRISDTV
                     KSIPNTPWAIFAVIGTYCLLSLFVFPISILLYGTILTFGLFPGMLYSFAGTMSAAILN
                     YYLGAWFSQHEVFRFLERRKLSDLKEALRNPSLATIMIIRLTPVAPFTVENYLAGAVG
                     VRPGLYILGTFLGLTPGILSIGLITTALSPYVQNPLYYIPIYFGMSSVLLALIVAGKK
                     IAGACARFSLHRRLGSIIYTKLEQIKGVDLYFKDHLWLASYISQVSYGIEGQSYFVKV
                     GGPNGSDALLLFQRITVTPKELLGYVKARGSLGKSVVRKVLQFFLQNRRITILVNGCP
                     NLTGDFRFVSKPDDLDDADALVAALRKLKNVIDFDAVIIKDIHPPLKKVPAEFKKFQV
                     DPNMVVHNTWPSVEAYVAAMRTKYRQRYQSTMRKSAAISMQKLNLTQIAEHKAEINGL
                     LAKVVKQDKFNLEQIPDDYFLRLAGMQQFSLYGYFLDDRLVAFRSSLEAADRLIAHYV
                     GFESDVNTEQKIYQRMLYDYVAEGIEKKLPAIHLGRTALEIKSTVGAEREDYYLMFHS
                     DNWFYRTVGNYYVSRLAATGYEARSPFRDE"
     gene            1930..>2234
                     /locus_tag="PLF85_16515"
     CDS             1930..>2234
                     /locus_tag="PLF85_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNE21325.1"
                     /translation="MMPNLVTIALAALIPMAVGFTWYHPKIFGTKWMNLAGLTEEKIK
                     DANMALIFILSYVFSLMMAMAIMSIVIHQGHVYSILVKTPGFGVPGSPVQTMLENFM"
BASE COUNT          555 a          566 c          609 g          504 t
ORIGIN      
        1 ggtcattcaa cgagtaagat tagcccacaa gacgcgctcc tccagtgccg gcatgtgata
       61 ctcactcatc ccgaaacggt gagcgcgcct cgtacccggt tgccgcaagg cggctcacgt
      121 agtagttgcc gacggtgcgg tagaaccagt tgtcagagtg gaacataagg taatagtctt
      181 cgcgctcggc gccgacggtt gatttgatct cgagcgccgt gcggcccagg tggatcgcgg
      241 gaagcttctt ttcaatacct tcggccacgt agtcgtaaag catgcgctgg taaatcttct
      301 gctcggtgtt cacatcgctt tcgaagccga cgtagtgcgc aatcagccgg tctgccgctt
      361 cgagggagct gcgaaaggca accaagcggt catccagaaa atagccgtag agagaaaact
      421 gctgcatacc cgcgagccgc agaaaatagt cgtcgggtat ttgctcgagg ttgaatttgt
      481 cttgtttgac gaccttcgcc aacagaccat tgatctcggc cttgtgctcg gctatctgcg
      541 tcaggttcag cttctgcatt gatatggcag cgctcttcct catcgtactc tggtagcgct
      601 gccggtactt ggtgcgcatc gcggcgacgt aggcctcgac gctcggccac gtattatgca
      661 ccaccatgtt ggggtctacc tggaattttt tgaactccgc aggcactttt ttgaggggcg
      721 gatgtatgtc tttgataatg acggcgtcga aatcgattac gtttttcagt ttcctcagcg
      781 cggcgacgag cgcgtctgca tcgtccaggt catcgggctt cgatacaaag cggaaatcac
      841 cggtgaggtt tgggcagcca ttcaccagga tagtgatgcg gcggttttgc agaaagaact
      901 gcagaacctt gcgcacgacg ctcttgccga gtgagccgcg cgccttgaca tagcccaaca
      961 gttctttcgg cgtcacggtg attctctgga acaaaagcag ggcatcggag ccgttcgggc
     1021 cgccgacctt gacgaaataa ctttgccctt cgatgccata actgacctgc gagatgtaag
     1081 aggccaacca caggtggtct ttgaaataca ggtcaacgcc tttgatctgc tcgagcttgg
     1141 tgtagataat cgagccaagc ctgcgatgca ggctaaaccg cgcacaagcg ccggcgatct
     1201 ttttgcctgc gacgatcaac gcgagcagca cgctcgacat gccgaagtag atcggtatat
     1261 aatagagcgg gttttgcacg tatggactga gcgccgtagt gatcagccca atactcagta
     1321 tacccggagt caggccgaga aacgtgccca atatatagag gcccggtcta actccgactg
     1381 cgccagcaag atagttttct acggtgaagg gcgcaaccgg cgtgagccga atgatcatga
     1441 tcgtggcgag actggggtta cgcagcgctt ctttgagatc agaaagtttg cgccgctcga
     1501 gaaatcgaaa tacctcgtgc tggctgaacc atgcgccaag atagtaattg agtatggctg
     1561 ccgacatggt accggcaaaa gaatagagca tgcctggaaa gagaccaaag gtaagaatgg
     1621 tgccatagag cagaatactg atcggaaaaa caaacaggct cagcaggcaa taggtaccga
     1681 tgaccgcaaa aatagcccaa ggggtattgg gaatactttt gactgtatcg ctgatgcgct
     1741 gcgccaccga tgcatcgaga aacttccagg tgatcgccag cgagagcagc agaagcaaag
     1801 gaaaaacaag tcgcagcaga tcggcaaagc gggcaaacct gccatggttc atcgaggcgt
     1861 tgtgccatcg gtcaacgcat cgtcaatgtg attgacgtgt acaataatca tcttaagcat
     1921 taaactaata tgatgcccaa tttagtaact atagcacttg cagcgcttat acccatggcg
     1981 gtaggcttca cctggtacca cccgaaaatt tttgggacaa aatggatgaa ccttgccggc
     2041 cttaccgaag agaagataaa agatgccaac atggcgctta tcttcattct gagctacgtg
     2101 ttttcgctca tgatggcaat ggcaattatg tcgattgtta tccaccaggg acacgtctac
     2161 tcgattctcg tgaagacacc aggcttcggt gttccgggat cacccgtaca aacgatgctc
     2221 gaaaatttta tgaa
//
LOCUS       DAOQJF010000458         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_37490_length_2229_cov_6.211132, whole genome shotgun sequence.
ACCESSION   DAOQJF010000458 DAOQJF010000000
VERSION     DAOQJF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37490_length_2229_cov_6.211132"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLF85_16520"
     CDS             complement(<1..485)
                     /locus_tag="PLF85_16520"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21326.1"
                     /translation="MESMTGYGESHFSVEGVQLVCSARSVNSRFLDVELHYPPELSWF
                     KSHTEEIIRSRFSRGRIEVGLTSSAPLPVDIAFNTDIIAQYERFLAQQTGKKKVNLDP
                     RDFLQLPGFMEKRVKNWRSYQNKFDFHLLRALIKMQRSRLAEGKRTTRVCLTHLRYLA
                     R"
     gene            complement(570..743)
                     /locus_tag="PLF85_16525"
     CDS             complement(570..743)
                     /locus_tag="PLF85_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21327.1"
                     /translation="MQGIPEISTRDAAQPTVISSEDLKALLYLTIRGQNGLTGSKEEP
                     QRIDSEKGVDIKA"
     gene            835..1602
                     /locus_tag="PLF85_16530"
     CDS             835..1602
                     /locus_tag="PLF85_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21328.1"
                     /translation="MRGALTFTLLLLLNAGLFAGPEVFDPLDAWKRPGGPAKRPVSET
                     VVAGKTTELARYQYNNNQISRIDYLSLNERASGPKEIPAGYTLFEYDKGILSREQLFD
                     AAGNMTEEIRYQYRAGKLDKTLIHDIRGNARIEWHYQYDKEGVLAGGKRLLAGKPTES
                     FKLVKTTTGWMQNIYNSKGELTAKVDSYFENGLLTQRVKSGLTGTRYADYRYNAEKSL
                     IEIIFHDTVRGEKTLVKKHTFSYSLDKVVPGTALLHH"
     gene            complement(1591..>2229)
                     /locus_tag="PLF85_16535"
     CDS             complement(1591..>2229)
                     /locus_tag="PLF85_16535"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNE21329.1"
                     /translation="YRIARDADVVLVDATNPFGSGKLLPQGILREPVENLARAHAIVL
                     TKTDLVEAGVLEELREKLQRLSGLSHIFISRHAVRGLVQIPHEYAPNFTPKAISTELL
                     RHETVWALSAIANSRAFENMLRRAGAERVEQISFRDHHDFSERDIRMVLERVRPYDLV
                     VTTEKDYVRLRKHMDQLKVLRQFYYLKIEFEVLENEILLKEGLKARLLHSVV"
BASE COUNT          511 a          615 c          610 g          493 t
ORIGIN      
        1 atgcgcgcga ggtagcgcag gtgcgtgagg cagacgcggg tggtgcgttt gccttccgca
       61 agccgtgagc gctgcatctt gatcagggcg cgcaaaaggt gaaaatcaaa cttgttctgg
      121 tatgaacgcc agttcttgac gcgcttttcc atgaaaccgg gtagctgcaa aaagtcgcgc
      181 gggtcgaggt ttaccttctt tttgccagtc tgctgcgcga gaaagcgttc gtactgcgcg
      241 atgatgtcgg tattgaaggc gatgtcgacg ggcagcggag ccgatgaggt aagaccaact
      301 tcgatgcgcc cgcgcgaaaa gcggctgcgg ataatttctt cggtatgaga tttaaaccag
      361 gaaagttcgg gagggtaatg cagctcaacg tccaggaacc ggctgttgac gctgcgcgcc
      421 gaacagacga gctgaactcc ttcgacgctg aaatggcttt cgccgtaacc tgtcatgctt
      481 tccataatgg ttcacgttaa cggccgggcg aattccggac gcaatctcct ggcgcagcct
      541 gaatcagagg ctggcggcca aagacaacgt caggccttaa tatccacgcc tttttcgctg
      601 tcgatacgct gtggctcttc cttagaccct gtaaggccgt tttgcccgcg aatggtaagg
      661 tacagaaggg ctttgaggtc ttctgacgaa atcacggtgg gctgtgcggc atcacgtgtg
      721 ctgatttctg gtatgccttg cataaactcc ctctccctca gagtgtcggt gggcactact
      781 ttagacttta agaaagttct catttttttt tcgcggctgg ccgaaatatg tcccatgagg
      841 ggagctctca cattcacact gcttttgctg ctaaacgccg gtcttttcgc aggccccgag
      901 gtctttgacc cgctcgacgc ctggaagcgc ccgggtggcc ccgctaaaag acccgtatca
      961 gaaaccgttg tcgcgggtaa aacgactgaa ctggcgcgct accaatacaa caataaccag
     1021 atctcgcgta ttgactacct cagtctcaat gaacgggcct ccggcccaaa agagataccg
     1081 gccggttata ccctgtttga atacgataag ggtatactgt cgcgcgaaca gctgtttgat
     1141 gctgccggca acatgaccga agaaatacgc taccagtacc gcgcgggcaa actggacaag
     1201 acgctgatcc acgacatacg cggcaatgcg cgtattgaat ggcactacca gtatgacaaa
     1261 gagggagttt tagcgggtgg caaacgcctg ctcgccggca agccgacaga atctttcaag
     1321 ctggtcaaga ccactactgg ctggatgcag aatatctaca attccaaggg cgaactgacg
     1381 gcaaaagtcg actcgtactt tgaaaacggt ctgctcacgc agcgcgtcaa gtcaggcctg
     1441 accggcacac gttatgccga ctaccggtac aatgcagaaa agtcgctgat cgagattatc
     1501 tttcatgaca cggtgcgcgg agagaagact ctcgtaaaaa agcacacctt cagctattcg
     1561 ctcgacaaag tcgtgcccgg cactgcgctg ctacaccact gagtgcagca ggcgggcctt
     1621 caggccctct ttcagtagta tctcgttttc caggacttca aactcaatct taagatagta
     1681 aaactggcgc aagaccttca actggtccat gtgcttgcgc agcctcacgt agtctttctc
     1741 tgttgtaacg acgagatcat agggtcgcac gcgttcgagc accatgcgga tatcgcgctc
     1801 cgaaaaatcg tggtggtcgc gaaagctgat ctgctctacc cgctcggcgc ccgcacggcg
     1861 cagcatattc tcaaacgccc ggctgttagc aatcgccgat aacgcccaga cggtttcatg
     1921 ccgcagcagc tctgtcgaga tagctttagg agtaaagttg ggcgcatact cgtgcggtat
     1981 ctgcaccagg ccccgcaccg cgtggcggct gataaaaatg tgcgatagac ccgacaggcg
     2041 ctgcaacttc tctcgcagct cttcgagaac acccgcttct accaggtcgg ttttggtgag
     2101 cacgatggcg tgcgcgcgcg ccaggttttc tacaggctcg cgtaatatgc cctgtggcaa
     2161 aagcttgccg ctgccaaacg ggtttgtcgc atccaccagc acaacatcgg cgtcccgcgc
     2221 aatgcggta
//
LOCUS       DAOQJF010000459         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_37758_length_2220_cov_5.309007, whole genome shotgun sequence.
ACCESSION   DAOQJF010000459 DAOQJF010000000
VERSION     DAOQJF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37758_length_2220_cov_5.309007"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLF85_16540"
     CDS             <1..429
                     /locus_tag="PLF85_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21330.1"
                     /translation="NFRAYISTSASDAACRILGLSGKLVSNCGLPVAPNLAALGPYRN
                     RNAQQVAANVQNFISNSLSAAVGFDENGSSVGSATPFTGTANGVQSGQNCSDWTDNAI
                     LSNGVVGSASSTTSTWSSNGNGACALLGLLPISPIYCVQY"
     gene            complement(431..1903)
                     /locus_tag="PLF85_16545"
     CDS             complement(431..1903)
                     /locus_tag="PLF85_16545"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE21331.1"
                     /translation="MATEKKKPDVTVAVIGAGFSGLGMAKKLQDAGIEDFVILEAAGE
                     VGGTWRDSKYPGAAVDVPNHLYSYSFAQNPDWTRMFSNSDEIQRYIVDIADRFDLRRK
                     IRFNTKIVEAHFDEVSNLWEAKAQDGTLFRARFVVGAMGPFAESRIPDVPGLKKFKGT
                     MAHTAKWDRNIDIRGKRVAVVGSGASAIQVVPAVQPLAKELKVFQRTPSWVVPKADYE
                     YSELEKMVYRYLPFTQKIRRAVIYGLTELLATAIVWDTPMTDLLEKISQRHREKAIKD
                     PELLRKVTPDYRLGRTRMLISNDWYPALAQPNVELIDHGVDSLTENGIVANGKEYPVD
                     IIIWATGYKSPSEGFPFPLTGAGGRDLNSYWANGAKAYKGVTIAGFPNLFTLMGPNTG
                     PGHTSVLVYVEAQQEYIVKAIQQCQKNHIGSLSVREDRQEEFNRELVEKMKKTTWGDG
                     GNSWYYTKDGRNTTLYPGFATDYVLSVRNFDLGDYSVKSI"
BASE COUNT          443 a          630 c          596 g          551 t
ORIGIN      
        1 aatttcaggg catatatctc gacttctgca agcgacgccg cctgcagaat tctcgggctc
       61 tcgggtaaat tggtgtcaaa ttgcggatta cctgttgcgc caaaccttgc cgcgctggga
      121 ccttatcgaa accgtaacgc acagcaggta gccgcgaacg tgcagaattt catctcgaat
      181 agcctgtctg cagccgttgg ctttgacgaa aacggtagca gtgtcggttc agcgacgcca
      241 ttcaccggta ccgccaatgg cgtgcagtca ggtcagaact gttcagactg gacagacaac
      301 gcgatactca gcaacggcgt cgtgggcagt gcgtcatcaa cgacctcgac gtggagctca
      361 aacggtaacg gcgcctgcgc gcttttaggc ctgttgccga tttcgcccat ctattgcgtg
      421 cagtattgag ttatatactt ttcacgctat aatcgccgag gtcgaagttg cgtacactca
      481 gcacgtaatc tgtcgcaaaa cccggataga gcgtggtgtt gcggccatct ttggtgtagt
      541 accagctgtt gcccccgtca ccccaggtcg ttttcttcat cttctcgacc agttcacggt
      601 tgaactcttc ctgccggtct tcgcgcacag acaggctgcc aatatggttc ttctggcact
      661 gctgaatcgc cttaacgatg tattcctgct gtgcttcgac gtataccagt accgaggtat
      721 ggcccgggcc ggtgttcgga cccatcaggg tgaacaggtt aggaaatccc gcgatggtaa
      781 ctcccttata ggccttcgct ccgttcgccc agtagctgtt caggtcgcgg ccacccgcgc
      841 cggtgagtgg aaacggaaaa ccttctgatg gcgacttgta tccggttgcc cagatgataa
      901 tgtctacggg gtattctttg ccgttggcaa cgataccgtt ttcggtgagg gaatcgacgc
      961 cgtgatcgat gagctccaca ttgggctgcg ccagtgccgg gtaccagtcg ttcgatatca
     1021 gcatacgtgt acggcccaag cggtaatctg gcgtgacctt gcgcaggagt tcgggatctt
     1081 taatcgcctt ttccctgtgc cgctgcgaga ttttttcaag caggtcggtc atcggcgtat
     1141 cccacacgat tgcggttgcg agcagttcgg tcagaccata gattaccgcg cgacggatct
     1201 tctgtgtgaa gggcaggtaa cgatagacca ttttttcgag ttcgctgtat tcgtagtcgg
     1261 ccttgggcac gacccacgaa ggtgtgcgct ggaatacctt gagctccttt gcgaggggct
     1321 gtactgcagg caccacctgg attgcgctgg caccgctgcc gacgactgcg acacgcttgc
     1381 ccctgatatc gatgttgcgg tcccatttgg cagtgtgcgc catggtgccc ttgaacttct
     1441 tcaggcccgg cacatcggga atgcgcgatt cagcaaacgg tcccatggcg ccgacgacga
     1501 atcgggcccg gaacagggtg ccatcctgcg ccttggcttc ccaaaggtta ctcacttcgt
     1561 caaagtgtgc ctcaacgatt ttcgtgttga agcggatctt gcgccgcagg tcaaaacggt
     1621 cggcgatatc gacgatatag cgttggattt catctgaatt ggagaacatt cgcgtccagt
     1681 cggggttctg cgcaaacgaa taagaataca ggtggttggg cacatcgacc gctgcaccgg
     1741 gatacttgct atcacgccag gtcccgccga cttcgccggc cgcttcgaga attacgaagt
     1801 cttcgatgcc cgcgtcctgc agttttttgg ccattcccag cccgctgaag cccgcgccga
     1861 tgacggctac tgttacgtcg ggtttcttct tttctgttgc catgttgcct cctcgctgct
     1921 gcagtccggt tataggcgga ttgtcgtttg tgcagcgtct aatacctata aaaagacccg
     1981 cttatcctgt aaataaaaaa gtgaattgtt gttcattttt aagccttatc ggttaccttc
     2041 ctcgcctcac gcaccaaagt gacccactgc gcgaaggctt tacctcctta aatattccac
     2101 cgccctcacc gcttgcgcaa aactgcttca gcctcactgc agccaaagag ccctgctggc
     2161 cgctcagccg cgcctcatgt aaccacgatc gggtagggag cttggcatgc ccttaaatac
//
LOCUS       DAOQJF010000460         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_38478_length_2199_cov_4.354478, whole genome shotgun sequence.
ACCESSION   DAOQJF010000460 DAOQJF010000000
VERSION     DAOQJF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38478_length_2199_cov_4.354478"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLF85_16550"
     CDS             <1..941
                     /locus_tag="PLF85_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802861.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNE21332.1"
                     /translation="SKLLQRAPEKFDMILDKSHPQYINLPGISVLVGGLWIANLYYWG
                     CNQYIIQRALAAKSIREAQTGLAFAGVLKLLLPLIVVIPGIVAFVITNDPKSGHFGEI
                     TKPDAAYPWLLHKFVPSGVKGLAFAALIAAIVSSLASMMNSISTIFTMDIYRGFFEPG
                     ASETKLVKVGRIAAGAALVVSVPVAIALQNLDQAFQFIQEFTGFVSPGALAIFLLGFF
                     TRRAGANGALLAALGTFIFSALLKAFVPQLPFLDRMLVVFVLCVIVMLLTPAKNADSN
                     TEQLQLEPGTFSTSTGFKLVAVGITAVLTVIYAAWW"
     gene            943..2091
                     /gene="galK"
                     /locus_tag="PLF85_16555"
     CDS             943..2091
                     /gene="galK"
                     /locus_tag="PLF85_16555"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNE21333.1"
                     /translation="MVHTGKIRHAFKERFGAEGEIFCAPGRVNLIGEHTDYNMGFVLP
                     GAIDKAITLAIRPNGLNQFRLLALDTGEEETFTVADKSTRHHWASYILGVVMEFQSRC
                     FAVQPFDAVFAGDIPQGGGMSSSAALESAFAFAINELGNFGVQRLELAQIGQSAEHKY
                     AGVRCGIMDQFASLHGAKDHLIRLDCRSLQYELIPFVLPGYRLLLLDTRVKHSLASSE
                     YNTRRAECETGVAILRKRFNSVQSLRDADLAMLESVRAELDQATYTRCQYVIEENDRV
                     LAAVEQLKLGDVAAFGRLMYASHEGLSQKYNVSCRELDLLVAVARETQGVQGARMMGG
                     GFGGCTINLVAESAIADFKRIVQEKFFAAFGHNPLVYEVAISEGARRP"
BASE COUNT          465 a          622 c          641 g          471 t
ORIGIN      
        1 tctcgaaact gctgcagcgc gcaccggaaa aattcgacat gattctcgac aagtcgcacc
       61 ctcagtatat caacctgccg ggcatcagcg tactcgtcgg cggcctgtgg atagccaacc
      121 tctattactg gggctgcaac cagtacatca tacagcgcgc gctggctgca aagagcattc
      181 gcgaagcgca gaccggcctg gcctttgcgg gagttttgaa actgctgctg ccattaatag
      241 tggtaatacc gggcattgtc gcgttcgtca tcacgaatga cccgaagagc ggccacttcg
      301 gcgaaatcac caaacccgac gccgcatacc cgtggctatt gcataagttt gtacccagcg
      361 gcgtcaaagg tctcgccttc gcggccctga tagcggccat tgtgagctcc ctcgcgtcga
      421 tgatgaacag tatctcgacg attttcacga tggatattta ccgcggcttt ttcgagcccg
      481 gcgccagcga aacaaaactg gtgaaggtcg gtcgcatcgc ggcgggtgct gcgcttgtgg
      541 tctcggtgcc ggtggcaatt gcgctgcaga acctcgacca ggcattccag tttatccagg
      601 agttcacagg ctttgtcagc cctggcgcgc tcgctatatt cctgcttggc ttttttaccc
      661 ggcgggcagg agcaaacggc gcgctgctgg cggcgctcgg gacatttatc ttctcagcgc
      721 tgctgaaagc gtttgtgccg cagctgccgt ttctcgatcg catgctggta gtcttcgtgc
      781 tctgcgtgat agtgatgctg ctcacgccgg caaagaacgc ggatagtaac actgaacagc
      841 tgcagcttga gcccggtact ttcagcacga gcacaggctt caagctggtg gcagtaggta
      901 ttacggcggt actcacggtg atttacgcag cctggtggta atatggtcca tacagggaaa
      961 atccgccacg ccttcaaaga gcgctttggc gcagagggtg aaattttctg tgcacccggt
     1021 cgggttaacc tgatcggcga gcacaccgac tacaatatgg ggtttgtgtt gcccggtgca
     1081 atcgacaagg ccatcacgct ggcgatacgg ccgaatggtt taaaccagtt tcgcctgctc
     1141 gcactcgata cgggcgaaga agaaactttt accgttgctg acaagagtac ccggcaccac
     1201 tgggccagct acatactggg cgtcgtcatg gaattccaga gccggtgttt tgctgtgcag
     1261 ccttttgatg cggtctttgc cggcgacatt ccacaggggg gtggcatgtc ttcttcggcg
     1321 gcgctcgaaa gtgcgttcgc tttcgcgatc aacgagctgg ggaacttcgg agtgcaacgg
     1381 cttgaactcg cgcagatcgg gcagagcgcc gaacacaaat atgcaggagt ccgctgcggt
     1441 atcatggacc agttcgcttc gctgcacggc gctaaggatc acctgatcag gctcgactgc
     1501 cgctcgctgc aatacgagct tatacccttt gtattgcccg gctaccgcct gctgctgctc
     1561 gatacgcgcg tgaaacattc gctggcatcg tcagagtaca acacgcggcg cgccgaatgc
     1621 gaaaccggtg tcgcaatcct gcgaaaacga ttcaactcag tccaaagcct gcgcgacgct
     1681 gacctggcaa tgctcgaaag tgtacgggcc gaactcgatc aggccacgta cacgcgctgc
     1741 cagtacgtga tcgaagaaaa cgaccgcgtg ctcgcagcgg tcgaacagct gaaactcggc
     1801 gatgtggctg cgtttggccg gctgatgtat gccagccatg agggtttgag ccagaaatac
     1861 aatgtcagct gcagagagct cgacctgctc gtggccgttg cccgcgaaac gcagggcgtg
     1921 cagggtgcac gcatgatggg cgggggcttc ggcggttgta cgatcaacct cgttgcagaa
     1981 tcagcgattg cggatttcaa aagaatcgtg caggaaaaat tcttcgccgc attcggccac
     2041 aatccgctgg tatatgaggt ggctatctcc gagggcgctc gccggccgta acagaagaat
     2101 ttccgtcacg cactcagccg ctgcctgatg gcattggccg gcaatggcgg cgaaaagaaa
     2161 tagccctgcg cctgctggca acccatatca gccagcatc
//
LOCUS       DAOQJF010000461         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_39123_length_2179_cov_4.594162, whole genome shotgun sequence.
ACCESSION   DAOQJF010000461 DAOQJF010000000
VERSION     DAOQJF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39123_length_2179_cov_4.594162"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1870
                     /locus_tag="PLF85_16560"
     CDS             242..1870
                     /locus_tag="PLF85_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE21334.1"
                     /translation="MRKIWRNVYNWGDTEKKIDPTIQHHIDHFKHLLHIEGEAGELPL
                     NANAQQEVTLAKKSRLNRLQLRELMKLVGSDNVQVDDFSRARHSFGKYYADILRLRMG
                     KGINPPDAVVYPRSEEDVIKVINYCNAKRIAVIPWGAGSSVTRALEAVKGGIALDMSR
                     HMTDILSLNAEDSTVTVEAGILGPDIEKYLNERGYTCGHFPQSFEFSTVGGWLAARGA
                     GQASTGYGRIEDMVVGLKAVTPAGVISAGHYPAASVGGDIRHFLMGSEGTLGVITRVT
                     LRVRKYNAANSKLKSYMFPSFEQAVTAMREMMQSQVMPPHFFRISDPEETEAGLGMKG
                     KDKGITGALLNALGFKKGGRSLMYAIFEGEPAQVALGAKTLGRIVRRNKGLSLGSYAT
                     RKWLEQRYSSAYMRDPMMDAGIRIDTLETSVHYSRLTELWQAVRAYIKKDGQTICLTH
                     ISHTYETGANLYFIFASPMQRKDELSTFEKFHAGIIDTFVAHGGTLSHHHGIGRLGAH
                     MLKHQHSPATLAAWRAVKKTLDPKGIMNPGALGL"
     gene            complement(1842..>2179)
                     /locus_tag="PLF85_16565"
     CDS             complement(1842..>2179)
                     /locus_tag="PLF85_16565"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE21335.1"
                     /translation="ILGVGQMVCLADECATIVTNDSGLMHLLSASKTPMISLFLSTVE
                     EFGFYPLNDHAQVLAARDIPCRPCNHKGLPECPKKHFRCGRELTADQVYLEVAKLLGP
                     IKAPALQDS"
BASE COUNT          486 a          626 c          630 g          437 t
ORIGIN      
        1 ggtagtgggc gtagaatacc ttgcacaaga ccgtatttcg atttttggcg aggcttcggc
       61 gcagccgttt ctttttccct catggggcct tggcatcagt atagccgccg gcgcacgata
      121 ctggttctaa cggctcctga taaaggagcc gttagaaggt tccgaaaacc gctaatttgc
      181 gcaccgagat tctgcaaacg ctaaaggctt gtccgcttgt ccgttgagtc ctcaacggaa
      241 catgcggaaa atctggcgga atgtctataa ctggggcgat acagaaaaga agatcgaccc
      301 caccatacag caccacatcg accattttaa gcacctgctg cacatcgaag gtgaagcggg
      361 ggagctcccc ctcaatgcca acgcgcagca ggaagtcaca ctcgccaaaa aaagccggct
      421 gaaccgcctg cagctgcgcg aactgatgaa gctcgtgggc agcgacaacg tgcaggtcga
      481 cgacttcagc cgcgcgcgcc attcgttcgg taaatactac gccgatatcc tgaggctgcg
      541 catgggtaaa ggtatcaacc cgcccgacgc ggtagtttac ccgcgcagcg aagaagacgt
      601 tatcaaagtt atcaactact gcaatgctaa gcgtatcgcg gtcattccgt ggggggctgg
      661 ttcatcggtc acgcgcgcgc tcgaagcagt gaagggtggc atcgcgctcg acatgagccg
      721 ccacatgacc gacatcctct cgctcaatgc agaagattcg actgtgactg tcgaagcagg
      781 tatcctgggg cccgatatcg agaagtacct caacgagcgc ggctacacct gcggacactt
      841 tccgcagtcg tttgaatttt caacagtcgg cggctggctg gcggcgcgtg gtgctggcca
      901 ggcttcgacc ggttacggtc gcattgaaga catggtggtg ggcctaaagg cagtcactcc
      961 ggcaggcgtg atcagcgcag ggcattaccc tgctgcttcg gtcggcggcg acatccggca
     1021 tttcctgatg ggcagcgaag gtacgctcgg ggtgatcacg cgcgtcacgc tgcgcgtgcg
     1081 caagtacaac gccgccaact caaaactgaa gtcgtacatg ttcccgtcgt ttgagcaggc
     1141 cgtgaccgcg atgcgcgaga tgatgcagtc gcaggtaatg ccaccacact tctttcgtat
     1201 ttcagaccct gaagagaccg aagcagggct cggcatgaag ggcaaagaca agggcatcac
     1261 cggcgcgctg ctgaatgctc ttggctttaa gaaaggcggg cgctcgctga tgtacgcgat
     1321 cttcgaaggc gaaccagccc aggtcgcgtt gggagctaaa acgcttggcc gcattgtccg
     1381 gcggaacaaa ggtctttcgc tcggctccta tgcgacacgc aagtggctcg agcagcggta
     1441 ctcgtctgcc tacatgcgtg acccgatgat ggatgccggc atacgcatcg atacgctcga
     1501 aacctcggtg cactattcac gcctcaccga actctggcag gccgtgcgcg catacatcaa
     1561 gaaggacgga cagacgatct gcctgacgca tatctcgcat acctatgaga caggtgccaa
     1621 cctctacttc atttttgctt caccgatgca gagaaaagac gagctttcta cttttgagaa
     1681 attccacgct ggtatcattg atacgttcgt ggcgcacggc ggcacgctct cgcatcacca
     1741 cggcatcggc cgcctcgggg ctcatatgct gaaacaccag cactcccctg caacgcttgc
     1801 cgcgtggcgg gccgtgaaga agacgctcga cccaaaaggt attatgaatc ctggagcgct
     1861 ggggctttaa tcggccccaa aagtttcgcc acttccaagt agacctgatc ggccgtaagt
     1921 tcgcggccgc agcgaaaatg ttttttcggg cattcgggca gccccttgtg gttgcagggc
     1981 cggcagggta tgtcgcgggc agccagcacc tgcgcgtggt cgttgagcgg gtaaaaaccg
     2041 aactcttcaa ccgtgctcag gaaaagcgag atcatcggcg tctttgacgc cgagagcagg
     2101 tgcatcagac cgctgtcgtt ggtcacgatc gtcgcacact cgtcggcgag gcagaccatc
     2161 tggcccacgc cgagtatac
//
LOCUS       DAOQJF010000462         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_39146_length_2178_cov_3.749882, whole genome shotgun sequence.
ACCESSION   DAOQJF010000462 DAOQJF010000000
VERSION     DAOQJF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39146_length_2178_cov_3.749882"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PLF85_16570"
     CDS             <1..718
                     /locus_tag="PLF85_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21336.1"
                     /translation="MHSSLADVLVYPEGVGGISKGFDKRYKLQHFSLSFTRMALKFKV
                     DIVPVATINGEYINPYSYRIEQINKTIQKVIKLPFLPVGPMLGLLPASPWAFYFGMPA
                     KLTYVMGEPIKIYEMTDKDPAKISKGELRHITNQVQQKMQNHIYESEAQFGRDPYQWD
                     ELIETWKSNLDQMLYALPSGWPLLFAEHDRQYEEHKAVELDYSNTGFLQALAKNPDKA
                     AYMVPFAGWIPLLRKAQLFS"
     gene            755..1240
                     /locus_tag="PLF85_16575"
     CDS             755..1240
                     /locus_tag="PLF85_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21337.1"
                     /translation="MKSTNAYAWALQFIVWTILLYVFLTGTGFGYVKRLSNHWAIQLA
                     VTLGGLVFLAAVLYALSQRLFAAESGEKPIGFFRGVLFAILDLLLDFFYIIGSTILIM
                     GGLLLSLIGISDVACAGSSVTEKTVPMLIIGLVAFFLGIALRSDDSLEAYAAMKEKID
                     R"
     gene            complement(1279..1821)
                     /locus_tag="PLF85_16580"
     CDS             complement(1279..1821)
                     /locus_tag="PLF85_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21338.1"
                     /translation="MKDSPCLKTWHIERHSNPWNGLTGDAAAEEATFTVRAVRQRPTA
                     PNMLAQPGGIAANDFFLYRSEKDRAVVVNARYFGGLTRQDVTGRPLRNGGVIGVATPA
                     ALKRWQPTELLGLLLEQQIILTYAHIEATVCLVSATENNGIYRAELTGEHVYYTNERN
                     VSGFNFQFELDRSTSEVRVK"
BASE COUNT          455 a          596 c          607 g          520 t
ORIGIN      
        1 gatgcactcg tcgctcgccg acgtgctggt gtatcccgaa ggtgtcggcg gcatcagcaa
       61 gggtttcgac aagcgctaca agctgcagca tttctctctc tcgttcacgc gtatggcgct
      121 caagttcaag gtcgatattg ttcccgttgc gacgatcaac ggtgagtata tcaatcccta
      181 cagctaccgc atcgagcaga tcaacaagac aatccaaaaa gtcatcaagc tgcctttcct
      241 gccggtgggc cctatgctcg gacttctgcc tgccagcccg tgggcgttct actttggcat
      301 gccggccaaa ctcacctacg tgatgggtga accgatcaag atctatgaga tgacagacaa
      361 agaccctgca aaaatcagca aaggggagct caggcacatc acgaaccagg tgcagcagaa
      421 gatgcaaaat cacatctacg aatcagaagc acagtttggc cgcgacccct accagtggga
      481 tgagctgatc gaaacctgga agagtaacct ggaccagatg ctgtacgcgc tgccgagtgg
      541 ttggccgctg ctgtttgctg aacacgaccg gcagtatgaa gagcacaaag ctgttgagct
      601 cgattacagc aacaccggtt tcttgcaggc gttggcgaag aaccctgaca aagcggcgta
      661 catggtgccg ttcgcgggct ggatacctct gctacgcaaa gcgcagcttt tttcctgatt
      721 tccaggccgc cggccgtcgc tagtcttgcc cggtatgaaa tcaacaaacg cttacgcatg
      781 ggcgctccaa tttattgtgt ggaccatctt gctgtatgtc tttcttacgg gcaccggttt
      841 tggctatgtc aagcggctct caaatcactg ggctattcag cttgctgtta cactgggagg
      901 cttggtattt cttgctgcag tgctctatgc actcagccag cgcctgttcg ctgccgaaag
      961 cggagaaaag cccatagggt ttttccgggg tgtacttttc gccatactcg acctgctact
     1021 tgacttcttt tacattatcg gcagtacgat cctgattatg ggtggtttac tgctctcgct
     1081 gattggcatc agcgacgtgg cctgtgccgg cagttcggtc accgaaaaaa ccgtgccgat
     1141 gctcatcatc ggcttggttg cgtttttcct gggaatcgcc ctgaggtctg acgactccct
     1201 ggaagcatac gctgccatga aagagaaaat tgaccggtag ccagtaaggc tggcgcaatt
     1261 cggcggggcg cgctgcgctt acttgacccg cacctctgat gtgcttctgt ctagttcgaa
     1321 ctggaaattg aatcccgaaa cattgcgttc gttcgtgtag tagacgtgtt cgcctgtcag
     1381 ctctgcgcgg tagataccat tgttttcggt ggcgctcaca aggcagacgg ttgcctcgat
     1441 gtgcgcgtag gtgagaatga tctgctgttc gagcaaaagc ccgagcagtt ctgtgggttg
     1501 ccagcgcttc agtgcagccg gtgtcgccac gccgatcact ccgccgttgc gcagcggccg
     1561 gcctgtcaca tcttgccgcg tgaggccacc aaagtagcgc gcattaacca cgacggccct
     1621 gtctttttca gagcggtaga gaaaaaaatc attcgcggca atgccacccg gttgtgcaag
     1681 catgttcggc gcggtgggac gctgcctcac tgcgcgcacg gtaaacgtcg cctcttcggc
     1741 ggcagcgtcg cccgtgagcc cgttccatgg gttgctgtgg cgttcaatgt gccaggtctt
     1801 gaggcagggg ctgtctttca tgctggtctt tcgggttttg gcagtgagtg ccggtagcag
     1861 aaaaaaacaa ccaacgatga tcacgggcat tggtcgtaat atgaaaaatc gcacgcgcac
     1921 tgcccgatga tgctcgcggc ggattatggc ctacgtcaac cgcaatcgct aacagtttcc
     1981 gcaagaagga aactgttagc aggcttccgg aaccgaaaaa gtgaaaagca ggccgcttcg
     2041 gccgctttag cagtcgtttg catatgcaaa ccacgtgacc cgtcttcggg tctccgcttc
     2101 ggtggctttt tgctttttcg ggggctttcc gaagcctgct aaccgcttgc cggttctggt
     2161 cagtgctgca gcccgtca
//
LOCUS       DAOQJF010000463         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_39299_length_2173_cov_5.423041, whole genome shotgun sequence.
ACCESSION   DAOQJF010000463 DAOQJF010000000
VERSION     DAOQJF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39299_length_2173_cov_5.423041"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLF85_16585"
     CDS             complement(<1..624)
                     /locus_tag="PLF85_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21339.1"
                     /translation="MLKWLKPDSSNSAVPGSSRPPWIQKISVPAGQQANPEGELVWKS
                     RLAEKLRALQPQNPQPTGVAGESDGRDFAFHVMPLDIFNASAPALFRQPPPAEPKRQA
                     ETHVNEEIETPAPAEVPQQNPVLQKQEQGGVQPNAHLEYRLAANEARQTVDTPYELSY
                     STIDYEKGDEFDLDALFENFGGSASQAWNPPEDAIEAIAENAKQQAAA"
     gene            complement(762..1634)
                     /locus_tag="PLF85_16590"
     CDS             complement(762..1634)
                     /locus_tag="PLF85_16590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNE21340.1"
                     /translation="MKVSILFLALFALIACRAPQARTNPQDPAQQARSEDPVMQDGAA
                     SDDKAAAPVSEGYASWYGKELQGRPTASGELFDMNKMTAAHRDFPMNSLVLVKNLENG
                     KKTMVKVNDRGPYVEGRVMDVSYSAARELGFAEKGTARVQLELVQAGEDNFLSKAEGA
                     APVKVGKKRKAAKEEEEIIEPVADLDEAADADEDDGKIKSAGSKKEKLVFLDGKKPKG
                     FTVQVGAFKKKVNAERHRDEILEAYPEKAFIATNGTWHFVCLGDFKTRKQAKTFLKKL
                     NDDGIEVMYRGKVS"
     gene            complement(1725..>2173)
                     /locus_tag="PLF85_16595"
     CDS             complement(1725..>2173)
                     /locus_tag="PLF85_16595"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21341.1"
                     /translation="SKIHELLSRYTLPENTFLVEYGAPYVLDVARAAIACSGTVTLEC
                     ALFGVPHLILYRSSWMSYAIFKRVIRIPYIGMVNVLAGKFVVQEFLQHRLTAQNIATE
                     LGKILHDETYRRAQEGELQRISKRLTAYEPAKQAARVLLQELKTKH"
BASE COUNT          453 a          610 c          562 g          548 t
ORIGIN      
        1 tgcagctgcc tgttgctttg cgttctccgc aattgcctca atcgcatcct cgggcgggtt
       61 ccacgcctgt gaggcagaac ctccgaagtt ttcaaacagg gcatcgaggt caaactcgtc
      121 gcccttttcg tagtcgattg tcgaataaga aagctcgtag ggggtatcca cggtctggcg
      181 cgcctcattt gccgcgaggc ggtactccag gtgcgcattc ggctgtaccc cgccctgctc
      241 ttgtttctgc aggacaggat tttgctgtgg cacctcggca ggtgcaggag tctctatctc
      301 ttcgttaacg tgagtttcgg cctggcgttt aggctctgcc ggaggcggct ggcggaatag
      361 ggccggcgcg gaggcattga agatgtccag cggcataaca tggaatgcga aatccctgcc
      421 gtcactttcg cccgcaacgc ccgtcggctg tggattttgt ggctgtagtg cgcgcagctt
      481 ctcggccagg cggcttttcc agaccagctc cccttccggg tttgcctgct ggcctgcagg
      541 tacagaaatc ttttgtatcc atggcggacg cgatgaccca ggcacggccg aatttgaaga
      601 atcaggtttt agccatttga gcatagttca ccgcgctgct aacacaacaa gtatcggcag
      661 attttccgca gggccttatc gccaaatttt ccccgtccgc ggtagcgggc ataaaaaagc
      721 ccggtagacc cgggctcttt tgaataaacc tggaggaaaa atcagctgac tttgccgcgg
      781 tacattacct cgataccgtc gtcgttcagc ttcttcagga atgtctttgc ctgcttgcgg
      841 gtcttaaaat cgccgaggca aacaaaatgc catgtaccgt tcgtggcaat aaacgctttc
      901 tcaggatacg cttccaggat ttcatcgcga tgacgctcag cgtttacttt tttcttaaaa
      961 gccccaacct ggactgtaaa gcccttgggt ttcttgccat cgagaaatac cagtttttct
     1021 ttttttgagc cagcagattt gattttgcca tcgtcctcgt ctgcatcggc agcttcgtcg
     1081 agatcggcca ccggctcgat aatctcctct tcttccttcg cggctttgcg cttcttgccc
     1141 actttcactg gcgccgcgcc ttctgccttc gaaagaaaat tatcttcgcc tgcctgcacc
     1201 agctctagct gcacgcgtgc cgtgcctttt tctgcaaaac caagttcgcg tgccgctgaa
     1261 tagctgacat ccatgacgcg accctcaaca tagggaccgc ggtcgttgac ttttaccatc
     1321 gtctttttgc cattctcgag attttttacg aggacgagcg aattcatcgg aaaatcgcgg
     1381 tgcgcagcag tcatcttatt catgtcaaac agctctccgc tggcagttgg ccggccctgc
     1441 agctctttgc cgtaccacga agcataccct tcgctgaccg gcgcggctgc tttatcatca
     1501 ctggcagcgc catcctgcat aacagggtct tcagaacgcg cctgctgcgc cggatcctgt
     1561 ggatttgtac gagcctgcgg cgcgcggcag gcgataagcg caaacaatgc cagaaaaaga
     1621 atagagactt tcatgttcta ttctcgtacc agacgggcgc agcaataagt ctttttttct
     1681 aacaggctcc tgaaaaagga gcctgaagca ttttccggaa actgctaatg ctttgttttg
     1741 agttcctgta gcaaaaccct cgcggcctgt ttagccggct cgtaggccgt gagccgcttt
     1801 gaaatgcgct ggagttcgcc ctcttgcgcg cggcggtaag tttcgtcatg cagtatcttg
     1861 ccgagctcag tcgcgatatt ctgtgccgtc agcctgtgct gcagaaactc ctgcacgacg
     1921 aatttaccag cgagcacgtt caccataccg atatacggta tacgtatgac gcgcttgaag
     1981 atcgcgtaag acatccagga agaacggtaa agaatcaggt gcggcacccc gaacagcgca
     2041 cattcgagcg tgaccgtacc tgaacaggca atggcagccc tggcgacgtc gagtacataa
     2101 ggcgccccgt actcaacgag aaaagtattc tcgggtagag tataacggga gagtaattcg
     2161 tgaatcttag aat
//
LOCUS       DAOQJF010000464         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_39365_length_2171_cov_3.989603, whole genome shotgun sequence.
ACCESSION   DAOQJF010000464 DAOQJF010000000
VERSION     DAOQJF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39365_length_2171_cov_3.989603"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PLF85_16600"
     CDS             complement(<1..1151)
                     /locus_tag="PLF85_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE21342.1"
                     /translation="MPIPSSRALYREFISQPLRRQTSDNPVNSQPGSFRVYLNLLGEH
                     RRAFIVIFVAACLTELLYLAVPVTTRHIIDGVLMNTQLAAADKTRLLMVIGGVLFGLL
                     VAAQGIDFWRRFFTAIVNGRFVRSLRLRLFSHLMRLPLGKVHELKTGGIVSRLTSDAE
                     NLTGLLQMAIISPGVAFLRIAITFGILIYWHLPLAIVASGFIPPLVYISLIYILRVRP
                     VYRSVSNDRSAQDARTSEVFSGIRAVKAFEMQQTEKRRYNTVQGLMFRKNIFASLRES
                     AVDGIWNFLIPVTSLLIMVIGGFFFLRGETSIGDLVAFQMYSGMLLYPVWRMVFSLSQ
                     IQKSMASLDRVLDMLALEGEQNTGELPMPAALREIRFTDVSFGYRENQSV"
     gene            1340..>2171
                     /locus_tag="PLF85_16605"
     CDS             1340..>2171
                     /locus_tag="PLF85_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HNE21343.1"
                     /translation="MDPLKELSHTVTELPRGGYLVQTPGGYFQFGAPPETIKDTMFLP
                     EGVPTVFVLPKHFFNWRKGISVAELEFPIYYNFFLKKRKTILICNNEQFVRFKTVFNE
                     AVFGPKNLDVSNDYMAGLEVPALRQEMKFFSSTFKFSDMVGFGIFNEFGRFTHRGVTI
                     SQDLEGNFSVAHGGKHLADIPGKIEYKTIYQIGERLHEPFVPPLFGVTCLGPSHGFDP
                     HENTSGFIIWLNHSGIMIDPPVNTTEWLEDSNVNPKFIDSIILTHCHADHDAGTFQKI
                     L"
BASE COUNT          527 a          611 c          589 g          444 t
ORIGIN      
        1 accgactggt tttcccggta gccaaaggac acgtctgtga accggatttc gcgcaaggcg
       61 gcaggcatgg gtagctcacc cgtattctgc tcaccctcga gcgcgagcat gtcgagcacg
      121 cggtcgagcg atgccatcga cttctgtatc tggctcagcg agaacaccat gcgccagacc
      181 gggtagagca gcatgccgct gtacatctgg aaagccacga ggtcgccgat cgaagtttcg
      241 cctcgcaaaa agaaaaagcc acctatgacc atgatcagca gcgaagtgac aggaatcagg
      301 aagttccaga tgccgtcgac cgctgattcg cgtagcgaag cgaagatgtt cttgcggaac
      361 atcaggccct gcacggtatt gtagcggcgc ttttccgtct gctgcatttc gaatgccttc
      421 accgcgcgga tgccgctgaa aacttcgctg gtgcgagcat cctgcgcgga gcggtcgtta
      481 ctgacagagc ggtacacggg tcttacgcgc aggatgtaga tgaggctgat gtagaccagc
      541 ggcggaataa aaccggaggc tacgattgcg agcggcaggt gccagtagat aagaatgcca
      601 aaggtaatcg cgatgcgcag gaacgccaca cccggcgaaa tgatcgccat ctgcaaaagc
      661 cccgtgaggt tttcggcgtc gctcgtcagc cgcgagacga tgccaccggt tttaagttcg
      721 tgcaccttgc cgagcggtag ccgcatcaga tgcgaaaaga gccgcagtcg cagagaacgg
      781 acaaacctgc cgttcacgat cgcggtgaaa aagcgccgcc agaagtcgat gccctgcgcg
      841 gcgaccagca gaccaaacaa cactccccct atcaccataa gcagccgggt cttgtcggca
      901 gctgccagct gtgtgttcat cagtacgccg tcgatgatgt gccgcgtagt gacaggaacc
      961 gccaggtaaa gcagttcggt gaggcaggcg gctacaaaga tgacaataaa tgcccggcgg
     1021 tgctcgccta gcaggttgag gtatacccgg aaactgccgg gctggctgtt caccgggttg
     1081 tcgctggttt gacggcgcag tggttgtgaa ataaattcgc ggtaaagcgc gcgcgaagaa
     1141 ggaatcggca tgaggttaca aaatagcagc cgtacggact attgacgaaa taattcaacc
     1201 gcgtccccgt tggaggttct gcccagctgc acagaatgcg acaataacac gattagacct
     1261 gcagtcgcaa aatacttgac tcggtggtta cagggcagca tttgcagcca ctctcgtcag
     1321 gactctagac aactcagcca tggacccttt aaaagaacta tcgcacacgg taaccgagtt
     1381 acctcgcggc ggctacctcg tgcagacgcc cggaggttat ttccagttcg gtgcgccgcc
     1441 cgaaaccatc aaggacacga tgttcctgcc cgagggggtt cctactgttt ttgttctgcc
     1501 gaaacacttc ttcaactggc gcaaaggcat cagcgtggcc gagctggagt ttcctatcta
     1561 ctacaacttc tttctcaaga agcgcaagac cattctcatt tgtaacaacg aacagtttgt
     1621 acgcttcaag accgtcttta acgaagccgt cttcgggccc aagaacctcg acgtttcgaa
     1681 cgactacatg gccggccttg aagttcccgc gctgcgccag gaaatgaaat ttttcagcag
     1741 cacgtttaaa ttttccgaca tggttggttt cggaattttc aacgagtttg gtcgcttcac
     1801 ccaccgcggt gtcacaatca gccaggacct cgaaggaaat ttctcggtag cgcacggcgg
     1861 caaacacctc gccgacatcc ccggtaaaat tgaatacaag accatctacc agattggtga
     1921 gcgtttgcac gaaccgtttg taccaccgct ctttggcgtc acctgcctcg ggcccagcca
     1981 cggcttcgat ccgcatgaaa acacttcagg tttcatcatc tggctgaacc acagcggtat
     2041 catgatcgac ccgccggtca atacgaccga gtggcttgaa gactcgaatg taaacccgaa
     2101 attcatcgac agtattattc tcacgcactg ccacgccgat cacgatgcgg gaacgttcca
     2161 gaagattctc g
//
LOCUS       DAOQJF010000465         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_39430_length_2169_cov_6.727058, whole genome shotgun sequence.
ACCESSION   DAOQJF010000465 DAOQJF010000000
VERSION     DAOQJF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39430_length_2169_cov_6.727058"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..497
                     /gene="rpmB"
                     /locus_tag="PLF85_16610"
                     /pseudo
     CDS             288..497
                     /gene="rpmB"
                     /locus_tag="PLF85_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017809470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
     gene            694..882
                     /locus_tag="PLF85_16615"
     CDS             694..882
                     /locus_tag="PLF85_16615"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNE21344.1"
                     /translation="MKITLNVSDELLAEAMKETGIKGKTELIHHALREVVAAASRRHL
                     AAMFGADKKASVAPRRRR"
     gene            860..1249
                     /locus_tag="PLF85_16620"
     CDS             860..1249
                     /locus_tag="PLF85_16620"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE21345.1"
                     /translation="MHQEDGAKVLVDTSVWIDFLHGKTTAQRMHNLLETNRVLVHDFV
                     EAELRVGQINKDRESFFRYLQNQPRAKVIAMPELIDFVERKSLYSRGLSFIDIALVIA
                     AIVENGWLWTHDRALHKLAEELAIDFH"
     gene            1264..>2169
                     /locus_tag="PLF85_16625"
     CDS             1264..>2169
                     /locus_tag="PLF85_16625"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNE21346.1"
                     /translation="MTKHETPILFIDRGKIRQNYLELKDALPPFRIAYAIKSNSHPEI
                     IEILRKEGSHFETASVAEIEKLLALGVKAEHIVLSNPVKAPQAIRRALEHGVSIQSFD
                     SVEELEKFKPHAGASTPLSRRVRPQLRIVVPNEGSMWPLSGKFGADEELWPAIFQYMK
                     ENEIPLDGVTFHPGSQCETIAGWDSALYLTWRCIEIAREYGLNPTTINIGGGLPVPMG
                     RKIPTVDEVGKVVLRHLKEWERKGFKPADLIVEPGRFISGSAGVLASKIIGVARREKT
                     WAFMDCGVFTGMMETIDGITYPMLFS"
BASE COUNT          568 a          559 c          552 g          490 t
ORIGIN      
        1 tctttgtaag cgatttctgt cagtgtttcg agaactgcgt caatgtcctt cttgctgtgg
       61 ccggtttttt cggcgagcgt tgagagcaac tgactcttgg aaaggggttt cttttctgcc
      121 ataatgtgac ataatggggt acactcgccg ccttgtaaag gaaaaagtac gcaaatacag
      181 ggatttattg agtaatccgc gtacgcggat tactcaataa acttactcta aaaaaacttt
      241 accctctatc tgaaacgcct actgtggcct ttccaaagga gatcactatg gccagaaaat
      301 gtcaactcac cggcaaagga accgcattcg gtaacaatgt gagccactca caccgtaaga
      361 cccgccggac cttcaaggcc aacgtgcaca aaaagcgcat cttccttcct gaagaaggcc
      421 gctatatcac gctgagcctt tctacccgcg cactgcgtac cctgcaaaag aagggcctcg
      481 ctgcgatgat gaaagacgcg cagaagtaat tttgccgcaa atagctgcgg cgagaaccgc
      541 agaacgaaaa ccccgcgctg cggggttttt ttatgggtta gccaaacaga ccctggagaa
      601 gagcaaatgc tgcatgagtg aacctgccag gaccaccagc tttgtatgcc atatttgtat
      661 tgacattccg accataccaa aagggaaaca ggcatgaaga tcactctcaa tgtttctgat
      721 gagcttctcg cagaggccat gaaagagacg ggaatcaagg gtaagacaga actcatccac
      781 cacgccctga gagaagtggt ggctgccgct tcgcgcaggc atctggctgc catgtttggc
      841 gccgacaaga aggcatcagt tgcaccaaga agacggcgct aaggttctgg ttgatacgtc
      901 tgtgtggatc gattttcttc atggaaaaac aaccgcacag agaatgcata acctgctgga
      961 gacgaaccgg gtgttagtcc acgattttgt ggaagccgag ctgcgtgtcg gacagatcaa
     1021 taaagatcga gaatcctttt ttcgatatct gcagaatcag ccacgagcaa aggtcatcgc
     1081 gatgccagaa ctcatcgatt tcgtcgaaag aaagtcattg tatagcaggg gactgtcgtt
     1141 cattgatatt gcgctggtga ttgcagctat tgtcgagaac ggttggctct ggactcatga
     1201 ccgcgccctg cacaaacttg ctgaagaact ggcgattgat tttcactaaa acacggacac
     1261 tctatgacaa aacacgaaac acccattctt tttatcgacc gcggtaaaat tcggcagaac
     1321 tacctggaac tcaaagacgc gctaccgcct ttccgtattg cttatgcaat caaatcgaat
     1381 tcgcatcccg aaattattga aatactcaga aaagagggat cgcattttga aacggcgtct
     1441 gttgccgaga tcgaaaagtt actcgcactc ggcgtgaaag cggaacacat cgtcttgtcg
     1501 aaccccgtta aagcaccgca ggcaatccgc cgggcgctcg agcacggcgt cagcatacag
     1561 agttttgatt ccgttgaaga gctggagaaa ttcaagcccc acgccggggc ttcgactccg
     1621 ctcagccgcc gggtacggcc ccagctgcgc atcgtggtgc ccaacgaagg ttcgatgtgg
     1681 ccgctctcgg gcaagtttgg cgccgacgaa gaactctggc ccgcaatctt ccaatacatg
     1741 aaagaaaacg agatacctct cgacggtgtc acgtttcatc cgggttcgca gtgcgaaacc
     1801 atcgcgggct gggacagcgc gctctacctt acctggcgct gcatcgagat tgcgcgtgag
     1861 tatggtctca acccgacaac catcaacatc ggcggtggct tacccgtacc catgggtcgc
     1921 aaaatcccta cggttgatga ggtcggtaaa gtagtactcc gccacctgaa agagtgggag
     1981 cgcaaaggtt tcaaacctgc agacctgatt gtcgaacccg gccgttttat ttcgggttca
     2041 gccggcgtac tcgcttcaaa aattattggt gtcgcgcggc gcgaaaaaac ctgggccttc
     2101 atggactgcg gagtttttac aggtatgatg gagacgatcg acggcatcac ttacccgatg
     2161 ctgttctca
//
LOCUS       DAOQJF010000466         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_39725_length_2161_cov_6.253561, whole genome shotgun sequence.
ACCESSION   DAOQJF010000466 DAOQJF010000000
VERSION     DAOQJF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39725_length_2161_cov_6.253561"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLF85_16630"
     CDS             complement(<1..572)
                     /locus_tag="PLF85_16630"
                     /inference="COORDINATES: protein motif:HMM:NF039306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HNE21347.1"
                     /translation="MIRNVIFSLLALSLVLPVAAQKKAAAKKAAPKAAAKEAPAPVQP
                     AAPAAAAPANAEEHSIDTAAANKYGVSEELVESLKRKRVLNDKDFEKKKEGGFVTGLP
                     LVNSDPNTGFGYGARLYYFYNGEKQDPLFRRTPYRHQVYAQFFQTTFGYQYHELNWDS
                     PYIMNSLFRVRSALVFEKNIWANYFGTGART"
     gene            complement(718..1824)
                     /locus_tag="PLF85_16635"
     CDS             complement(718..1824)
                     /locus_tag="PLF85_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21348.1"
                     /translation="MSLRIVPLGNSKKEVKSFAYFMKEVYRDYPHFVPPIYSELIKFI
                     HSGPFNHIGEKQLYLAYRGDKIVARLSVHRNFAHNEHYKANQGFFGFFEALDDQEAVS
                     AIFAEGEKWLKERGCTSVLGPMNFAVYDEIGLLVDAFDMDPVLLCTYNPPYYLKLLEN
                     AGYAKEVDWYAYLKDTTIPNFMRLINGRVMKQPGMVIREVNRKNIDAEAAAVKHIFNQ
                     AWAENWGNVPFTEEQWLHLVKELKMIINEKLAFITELNGEPIAFAISLIDANQAVKKA
                     NGSLFPFGFIRLLLAMKKIKKIRTTIMGVLKEHRNRGIEIAMIHKTMVNGIAMGITEA
                     DCSLIVETNHAMITVLEKIGCIRYKTFRVLKKNI"
     gene            complement(1894..>2161)
                     /locus_tag="PLF85_16640"
     CDS             complement(1894..>2161)
                     /locus_tag="PLF85_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21349.1"
                     /translation="PCCPLVGCAVVLPAVAGGRVPGVVVAPVPGRAGAGLVAAGVVAA
                     GRAGAAVDGAPAGGRAGCAHNTAVMTVKRENPTHSFTFDYRFSA"
BASE COUNT          464 a          577 c          597 g          523 t
ORIGIN      
        1 gtgcgcgcgc cggtaccgaa atagttcgcc cagatattct tttcaaacac gagcgccgag
       61 cgcacgcgaa agagcgagtt cataatgtag ggtgagtccc agttgagctc gtggtactgg
      121 tagccgaacg tagtctggaa aaactgcgcg tagacctgat ggcggtacgg ggtacggcgg
      181 aagagaggat cttgtttttc accgttgtaa aagtagtaga gacgtgcacc gtagccgaag
      241 ccggtgttgg ggtctgaatt caccagcggc agaccggtaa caaaaccgcc ttcttttttc
      301 ttctcaaaat ccttatcgtt gagaacgcgt ttacgcttca aagattcgac gagttcttca
      361 gaaacgccgt atttgtttgc cgcggcagta tctatgctat gctcttcagc gtttgccggt
      421 gcggctgcag caggtgctgc cggttgtaca ggggcagggg cttctttcgc cgctgccttg
      481 ggggctgctt ttttcgccgc ggcctttttc tgtgctgcca ctggcaacac caggctgagc
      541 gccagcaggc tgaaaattac gtttcttatc atgtggtctc ctgagatgag agttccggtt
      601 ctttgtctgt taccggtcgt ccaggggaga tcactttagt cgatcgtcaa gtcagaaaac
      661 cggcaaagca gggcaaatcg cagagagcga ggacaccccg attcgcgcag agggtgatca
      721 aatgttcttc ttcaataccc ggaaggtctt gtaacgaata cagccgatct tttcgagtac
      781 ggtaatcatt gcgtggttcg tttcgacaat cagcgagcag tctgcctcgg taatgcccat
      841 ggcaatacca tttaccatgg tcttatggat catcgcaatc tctataccgc ggttacggtg
      901 ttctttgagc acgcccatga tcgtggtacg gatcttcttg atcttcttca tcgccagcaa
      961 caggcgaatg aaaccgaacg gaaacaggct gccattggct ttcttaaccg cctggttggc
     1021 gtcgatcagg ctgatcgcaa atgcgatggg ttcgccgttc agctcggtga tgaatgccag
     1081 tttctcgttg atgatcatct tcagctcttt caccaggtga agccactgct cttcggtaaa
     1141 gggaacgtta ccccagttct ccgcccacgc ctggttgaag atatgcttca ctgcggcagc
     1201 ttcggcatcg atgtttttac gattcacctc tctgatgacc atacccggct gtttcatcac
     1261 cctgccgttg atcaggcgca tgaagttcgg tattgtggtg tctttcaggt aggcgtacca
     1321 atcgacctct ttggcgtacc cggcgttttc gagcagtttc aggtagtagg gtggattata
     1381 cgtgcacagc agcacaggat ccatgtcgaa tgcatcaacc agcaggccga tttcgtcata
     1441 gaccgcaaag ttcattggcc ccagaaccga agtacatccc cgttctttga gccacttttc
     1501 gccctcggca aaaatggcac tcacagcctc ctggtcgtcg agcgcctcga agaaaccaaa
     1561 aaagccctgg ttggccttgt agtgctcgtt gtgcgcaaag ttgcggtgta ccgagaggcg
     1621 ggccacgatc ttgtcgccgc ggtacgccag gtagagctgc ttttcgccga tgtggttgaa
     1681 ggggcctgag tggataaact tgatcagctc agaatagatc ggcggcacga agtgcgggta
     1741 gtcacggtag acttctttca tgaaataggc gaaacttttg acttctttct ttgaattgcc
     1801 aagcggcacg atgcgcagag acatgggcac atgtctgtgg cggcgacacg ctacgaaaag
     1861 gtgatttttg cctgccactg ggtcttatgc tttttatgca gaaaaccgat aatcaaacgt
     1921 gaagctatgc gtgggatttt ctctttttac ggtcatgacg gccgtattgt gggcacagcc
     1981 cgcacggccg ccggcgggag cgccatcaac tgcagcccct gcgcggccag cggccaccac
     2041 gcccgcggca acgagtccag ctcctgcgcg acccggcacc ggagcgacca caacccccgg
     2101 aacgcggcca ccggccacag caggcagtac aaccgcgcag ccaaccaacg ggcagcaggg
     2161 t
//
LOCUS       DAOQJF010000467         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_40180_length_2147_cov_4.935946, whole genome shotgun sequence.
ACCESSION   DAOQJF010000467 DAOQJF010000000
VERSION     DAOQJF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40180_length_2147_cov_4.935946"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..233)
                     /locus_tag="PLF85_16645"
     CDS             complement(3..233)
                     /locus_tag="PLF85_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21350.1"
                     /translation="MRKPSIAASRNKFRKVLSQYVKHQGLDLEVTLKTGERVTLSKMR
                     VIEGDNVVSLTARGGRSILAIRDIQHLEVYVN"
     gene            complement(440..937)
                     /locus_tag="PLF85_16650"
     CDS             complement(440..937)
                     /locus_tag="PLF85_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE21351.1"
                     /translation="MSLLKPWTPEGREALLSEMNNRMNALQREFNQMFDNLSGWRSSP
                     IVLESEKFFNRFVPSINVRENDETVVVTAELPGLEEKDIHVTHSDSELILEGEKKIEK
                     RDEKDKDHIIESSYGTFRRVISMPGYIDMAKIDAKFKNGVLTITLPKSDKAKANYKKV
                     EVKAG"
     gene            complement(1049..1411)
                     /locus_tag="PLF85_16655"
     CDS             complement(1049..1411)
                     /locus_tag="PLF85_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21352.1"
                     /translation="MRLAIRTLRLTMFRNGVRRGVKRSYNRQQGPFEVVTTRFTMAEY
                     DTLHCAAAAMRVSVSWLVYRMVRLWLKPERRWQGNTHVTNYDCDSLKWNENAGVITEC
                     LLFWSKFPRASHSSCRIT"
     gene            1598..1670
                     /locus_tag="PLF85_16660"
     tRNA            1598..1670
                     /locus_tag="PLF85_16660"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1631..1633,aa:Phe,seq:gaa)
     gene            1807..2147
                     /gene="ssrA"
                     /locus_tag="PLF85_16665"
     tmRNA           1807..2147
                     /gene="ssrA"
                     /locus_tag="PLF85_16665"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          450 a          530 c          576 g          591 t
ORIGIN      
        1 tttcaattta catacacttc caggtgctga atatcgcgga ttgcgagaat tgagcggcca
       61 ccgcgggccg taagcgagac aacattatct ccctctatta cccgcatttt tgaaagggtt
      121 acccgttcac ccgttttgag ggttacttcg agatcgagcc cctggtgttt gacgtattgc
      181 gaaagtactt ttctgaactt gttgcggctg gccgcgatag aaggtttgcg catggatact
      241 cctggaaatt aggtgcggca tccatgcctg ttggtaatgc ctgaaagggg ccgagtagtt
      301 actcatctct tttcagctat caccggtgcg cgccgtcctt ggcgcgtgtc atgtgacata
      361 attagtatcg gcgcatcgtg ggatagcttg agcggaaaat cccccttttc ggggcgaaag
      421 ccccgaaaag gagaaaatat cagccagcct tgacttcgac tttcttgtag ttggctttcg
      481 ccttgtcgga tttcggcagg gtgatggtca gaacgccgtt cttgaatttg gcgtcgattt
      541 tcgccatgtc gatatagccc ggcatcgaga tcacgcggcg gaacgtaccg taagagcttt
      601 cgatgatgtg gtctttatcc ttttcatcac gtttttcgat cttcttttcg ccctcgagga
      661 tcaactcgct gtcagaatgc gtgacgtgga tgtctttttc ttcgaggccc gggagctcag
      721 cggtaacaac gacggtctcg tcgttttcgc gtacgttgat cgatggcacg aaacggttaa
      781 agaatttctc cgattcgagc actatcgggc tcgagcgcca gcccgatagg ttgtcgaaca
      841 tctggttgaa ttcgcgctgc agcgcgttca tgcggttgtt catttctgaa agcagggctt
      901 ctctgccttc tggggtccat ggtttgagta ggctcatata tcctcctgtg aattgagcgg
      961 cgaatgcggt gcacagttcg ccttgatgcg tgcgcatacc atatagggtt gcgcgtcaag
     1021 tacaatggat ggatttgtcg gttattggtc aggttattcg gcaggaggag tggctggccc
     1081 tggggaattt tgaccaaaaa agcagacact ccgtgatgac accggcattt tcgttccatt
     1141 tgagtgaatc gcagtcatag ttagtcacat gagtattgcc ctgccaccgc cgttccggtt
     1201 tgagccatag tctgaccatt cggtacacca gccatgaaac gctgacgcgc attgctgctg
     1261 ccgcgcagtg cagggtgtcg tattccgcca tggtgaagcg cgtcgtgacc acctcaaacg
     1321 gcccctgctg ccggttgtag ctccgcttaa ccccgcggcg aaccccgttt cggaacatag
     1381 ttagtcgcag ggtgcggatg gcgaggcgca tgacctccgt ctggtgtctg cgccagatct
     1441 gtgcctcggt ggctggtagt gatacgctgg tagtcaacat tgtccctcta ctatttaaat
     1501 accaggaaat gcccgaattt tctcattctg aatggctttt tttgctttac cctctgattt
     1561 gccgtgtgtt tcctccgtac ggaaattttt tcctgctggc cagatagctc agtcggtaga
     1621 gcagaggact gaaaatcctc gtgtcgggag ttcgattctc tctctggcca cattttttct
     1681 tctcttatct ctccagattc agataaattg gtcgaattcc ggagtattta gtatacttct
     1741 tctaacctag taacgggtag cccgaaggca acccgtgctg gttctgctac aggcagaacc
     1801 agtttagggg acgcattggc ttcgattgcc gcgttggttg tttgaaaagc atgcacggta
     1861 gcctccatcc aaaaggcaat ctgtataact gcagaaaacg aaacttacgc actcgcagct
     1921 taatcgctcg atgcactggt aatagcctga gtctgcggaa gctattgcct taaacctaca
     1981 gcagaatagg ttatgcgcgc gcggcgtgcg tgtggccaaa gttaagccgc taggcttcgt
     2041 tattgcctgt cgatgggcga tgcggagaag aaaaacaatt atcgactaag catgtagaaa
     2101 gcgaatgatt gagcggtaag acgcgggttc gactcccgcc gtctcca
//
LOCUS       DAOQJF010000468         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_41374_length_2113_cov_4.659378, whole genome shotgun sequence.
ACCESSION   DAOQJF010000468 DAOQJF010000000
VERSION     DAOQJF010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41374_length_2113_cov_4.659378"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLF85_16670"
     CDS             complement(<1..774)
                     /locus_tag="PLF85_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNE21353.1"
                     /translation="MANKYVYFFSAARTEGGAGDKKLLGGKGANLAEMTTLGIPVPPG
                     FTISTEVCTYYYAHGKQYPADLQGQIDQGIAELETAIGKKFGDQKNPLLVSVRSGAPV
                     SMPGMMDTILNLGLNDETVAGLIASTGNDRFAYDSYRRFIQMYGNVVMGMKHHDFEHI
                     LDGVKKAKGVKEDLALGAEDLKNLIVQYKAEVKKVTGKDFPQSPKEQLMGAIDAVFAS
                     WMNDRAIIYRKLNNISEALGTAVNVQSMVFGNRGDRSGTG"
     gene            907..>2113
                     /locus_tag="PLF85_16675"
     CDS             907..>2113
                     /locus_tag="PLF85_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21354.1"
                     /translation="MSFSALNTRPKPALAVRLTMTLGLIMLAVGALFSAIYFVLSVSL
                     FSRLANQQLDQKAKSAEVILKAVGNSRESVNSMTYIRNLQNAGFSDSIAVYSDKWKLV
                     AHSDEGLATALGRYATDTKLKLPEVEEQNKLLQDILKYRRGKPASPFYTVGNVNYIAY
                     TQDNTTVLLGLVAQKPKLLTLAASFFILCLLAAGINTLFVYHYGHSLAQSLAQIVRGL
                     DVDSLRPESIDAQYQEVDALLHTVDERIRQTRTEVAPQAAVTADNTAQLLQNKLFEKP
                     FPRMQQYELAVYPRRPRAETHEFISGAQDQGHVDVLIGVTESDQIDALVVKHRLQERF
                     WALAKANLSADDLARNLWSAFFAHSELVPGIFFARLDEAAHTMDIYRAGGVYLFEVTA
                     EGTCSEITLG"
BASE COUNT          435 a          584 c          597 g          497 t
ORIGIN      
        1 acctgtgccg gaacggtcgc cgcggttgcc gaagaccatc gactgcacgt tgactgcagt
       61 gccgagcgcc tcggagatat tgttgagctt gcggtagata atggcgcggt cgttcatcca
      121 tgaagcgaag acggcgtcga tggcgcccat cagctgctct ttgggcgact ggggaaaatc
      181 tttgcccgtg acttttttta cctcggcctt gtactgcacg atgaggttct tgagatcttc
      241 agcgccgagc gccaggtcct cttttacacc cttagccttc ttgacgccgt cgagtatgtg
      301 ctcgaagtca tggtgcttca tgcccatcac cacgttaccg tacatctgta tgaaccggcg
      361 gtagctgtcg tacgcaaagc gatcgttacc ggtgctggcg atcaaaccgg ccacggtttc
      421 gtcattgagg ccgaggttga ggatggtatc catcatgccg ggcatcgaca cgggggcacc
      481 ggagcgcaca gagaccagca gggggttctt ttggtcgccg aatttcttgc caatggcggt
      541 ttcgagttcg gcgattccct ggtcgatctg cccctgcagg tcagcagggt attgcttgcc
      601 gtgcgcatag tagtacgtac agacctctgt cgagatggta aaacccgggg ggacagggat
      661 tccgagggtg gtcatctccg caaggttggc gcccttgccg ccgaggagct ttttgtcccc
      721 tgcgccacct tcggtgcggg ctgcagaaaa gaagtaaacg tacttgttcg ccatggtgca
      781 gaacggggct ttttgacgcc ctgtaaatga ttaaggaggg tggttgccgg tttataggcc
      841 cgccgcaggt caggggtatc aattggttca aatttgacgg cgcgtctgcc gatgctatag
      901 gtacgtatgt ctttttccgc cctgaatacg agacctaaac cagcgttggc agtcaggtta
      961 accatgaccc tcgggctcat catgctggcc gtgggcgccc ttttttccgc gatttatttc
     1021 gtcctttcgg tctcactgtt ttcacgcctg gcgaaccagc agctcgacca aaaagccaaa
     1081 agtgcggagg ttatactgaa agcggtaggc aattcgcgtg aaagcgtgaa ttccatgacc
     1141 tacatccgta acctgcagaa tgcagggttc agcgactcta tcgccgtcta ttccgacaag
     1201 tggaagctcg tcgctcacag tgatgagggt ctggccactg cactggggcg ttacgcaact
     1261 gatacaaaac tcaagctgcc cgaggtcgag gagcagaaca agcttctgca ggatattctg
     1321 aagtaccggc ggggcaaacc tgcaagtccg ttttatactg taggcaatgt gaactacatt
     1381 gcctacaccc aggataacac tacggttctt ttggggctgg ttgcccagaa acccaagcta
     1441 ctgacgcttg ccgcttcttt ctttatactc tgcctgcttg ctgcgggcat caatacgctc
     1501 tttgtctatc attatggcca ttcgctcgcg cagtctctgg cacagatcgt gcgcggtctc
     1561 gatgtcgact cgctgcggcc agagagcatc gacgcgcagt accaggaagt ggatgcacta
     1621 ctgcatactg ttgacgaacg cattcggcaa acgcgcacag aagtcgctcc ccaggcagcg
     1681 gtaaccgcag acaatactgc ccagctgctg cagaacaaat tgttcgagaa gccctttccc
     1741 cgtatgcagc agtatgaact tgctgtatac ccacgccggc cgcgcgccga aacgcacgaa
     1801 ttcatctcgg gtgcccagga ccagggccat gtcgatgtgc tcattggcgt gaccgagtca
     1861 gatcagattg atgcactggt cgtgaaacac cgcctgcaag agcgtttctg ggcgctggca
     1921 aaggccaatc tttcagccga tgacctcgcc cgcaacctgt ggagcgcgtt ctttgcgcac
     1981 agcgagctcg taccaggaat tttctttgcc cggctcgatg aggctgccca cacgatggat
     2041 atttatcgtg ccggcggtgt ctacctgttt gaagtgactg ccgaaggtac ctgcagcgaa
     2101 atcaccctgg gca
//
LOCUS       DAOQJF010000469         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_41507_length_2109_cov_4.690847, whole genome shotgun sequence.
ACCESSION   DAOQJF010000469 DAOQJF010000000
VERSION     DAOQJF010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41507_length_2109_cov_4.690847"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1442
                     /locus_tag="PLF85_16680"
     CDS             201..1442
                     /locus_tag="PLF85_16680"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNE21355.1"
                     /translation="MAEVFVVDAIRTPRARGNKRGSLSDVHPQELVAGLLKELPKRNK
                     DLKVEKVNDVIMGCVTQVGDQAACIARYGVMAAHWPQDVPGYTVNRFCGSGLQAVNDA
                     AAYIQSGTFDLVVAGGVESMSRCKMGSDAGETTEENMATAMSIGNPKIAAHYNLVPQG
                     ISADIIATKYNITREEADRFAESSQQKADAAIKDGRFKNSVIPVVKDDGTVVDKDEYP
                     NPKWDFTYLSKMPAMFKGVGEKVFDAMALKTYPELGKINHIHGGGNASGVVDGAGIVL
                     LASEKAVKEHGLKKRARIVAMAVSGEEPTIMLTGPVSASKFALEKAGLKVEDIDLWEI
                     NEAFAAVPLYVQKQLNVPMDRINVNGGAIALGHPLGGTGAILLGTAVDELERRKARYA
                     LITLCIGNGMGIATIIERVEG"
     gene            1574..1837
                     /locus_tag="PLF85_16685"
     CDS             1574..1837
                     /locus_tag="PLF85_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21356.1"
                     /translation="MKKMIIAMAIALASVSGILAEETTGEKPATAGSPEGPAANANER
                     AKQNAEKRRERKRVHQKEREERKAERQARKEERRKQRQGKEGK"
     gene            1922..>2109
                     /locus_tag="PLF85_16690"
     CDS             1922..>2109
                     /locus_tag="PLF85_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21357.1"
                     /translation="MRIVKIAALATLFSGFAPLAAIEQSDWSYSAGIAGTYLNSGGND
                     AQTFYWGPYATVGLQATE"
BASE COUNT          523 a          551 c          614 g          421 t
ORIGIN      
        1 cagtaacgca aagcgctcca acccggccaa gcgtaatacc atgacggcac gcctcattag
       61 gagcggcagt tgcgtcgatg gttttcgacg cgtttttggt ttacatcctg aggccgggcc
      121 gggtggtatc cgtaacgtgt atatgcacgt ataaaacagg gcggggtacc ccaggtgccg
      181 cagcccaaag gcaggttacc atggctgaag tatttgtcgt cgatgcaatc cgcacgccgc
      241 gcgcgcgtgg aaataaacgg gggtctctct cagatgtcca tccgcaggaa ctcgttgcgg
      301 ggcttttgaa agaactaccg aagcgtaata aagatcttaa agtagaaaaa gtaaatgatg
      361 tgatcatggg ttgcgttacg caggtgggcg accaggctgc gtgtattgct cgctatggcg
      421 tgatggctgc ccactggcca caggatgtgc caggctatac cgtgaaccgt ttctgtggtt
      481 cgggtctgca ggctgtgaac gatgcggctg cttatatcca aagcggtact tttgatctgg
      541 tggttgccgg cggcgtagaa tcgatgagcc gctgcaaaat gggttctgac gcaggcgaaa
      601 ccacagaaga aaacatggcg acagcgatgt cgattggcaa cccgaaaatt gctgcgcact
      661 acaacctcgt gccacaggga atctcggctg atattatcgc gacgaagtac aacatcaccc
      721 gcgaagaggc cgaccgtttt gctgagtctt ctcagcagaa ggctgatgcc gcgatcaagg
      781 atggtcgctt caagaactcc gtcatacctg ttgtcaaaga cgacggtacg gttgtagaca
      841 aagacgaata cccgaatccg aagtgggatt tcacctacct ttcaaagatg cccgcgatgt
      901 tcaagggcgt aggcgaaaaa gtattcgatg ccatggcgct caagacttac cccgaactcg
      961 gtaagatcaa ccatatccat ggtggcggta atgcttcggg tgttgtcgac ggtgcaggca
     1021 tcgtgctgct cgcttctgaa aaagcagtta aggaacatgg actgaagaag cgtgcgcgca
     1081 tcgtcgcgat ggcagtctcg ggtgaagaac ccaccatcat gctcacgggg cccgtgtccg
     1141 catcgaagtt tgcgctcgaa aaagcaggac tcaaggtcga ggatatcgat ctttgggaaa
     1201 tcaatgaagc atttgccgct gttccgctct atgtgcagaa gcagctgaat gtccccatgg
     1261 accgcatcaa cgtcaatggc ggggctatcg cgctgggtca tccgctcggt ggtacgggtg
     1321 caatcttgct cggcacagcg gtcgatgaac tcgagcgccg caaggcacgc tacgcgctga
     1381 tcacactgtg cattggcaat ggcatgggaa tcgcgacgat tatcgagcga gttgaaggct
     1441 aaccaagggg agttttgact ctgtctaaac cccggcactg tatgccgggg tttttttgtg
     1501 gccgcaccga ccctcagaag cgtactattc aacagatact acggtgggta tatcgggtgt
     1561 caaaaggagc aacatgaaaa aaatgatcat cgcgatggca attgccttag cctctgtttc
     1621 aggcattctg gccgaagaga caaccggcga aaagcccgca acggcaggca gccctgaagg
     1681 tcccgctgca aacgccaacg agcgcgcgaa gcagaacgcg gaaaagcgcc gcgagcgcaa
     1741 gcgcgtccac cagaaagaac gcgaagagcg taaggccgag cgtcaggcac gcaaagaaga
     1801 acgccgcaaa cagcgccagg gcaaagaagg caagtaacaa gcctgcatcc ccgggcagac
     1861 cgtcagggct gcccattcac ctttcggtga gaccattagc atcgatgaac cctccgtaca
     1921 aatgagaatt gttaagatcg cggccttggc cactctgttc tccggctttg ccccgctggc
     1981 agccatcgaa cagtccgact ggtcttattc agctggcatc gcgggcactt acctcaacag
     2041 tggaggcaac gacgcgcaga cattctactg gggtccgtat gcaaccgtgg gactgcaggc
     2101 gacagagag
//
LOCUS       DAOQJF010000470         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_41967_length_2097_cov_3.874633, whole genome shotgun sequence.
ACCESSION   DAOQJF010000470 DAOQJF010000000
VERSION     DAOQJF010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41967_length_2097_cov_3.874633"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLF85_16695"
     CDS             <1..1114
                     /locus_tag="PLF85_16695"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNE21358.1"
                     /translation="TPTIKAYVKEKKLAGGLMVSASHNPAEYNALKFIKKDGFFFDAD
                     DNSRFVRALEAKADWGTYRKQGKIIEAHAQAIDVHVKSILRVVKKPRRRLRVVIDPVG
                     SCGGEIALALLARLGAQVTAIHLEEVPEFPRPPEPVAAALKKLAAAVKKHKADIGFAF
                     DPDADRLSLVDEKGRAIGEEYTLPLAILSAARPGSTIVANLSTSMLTERAALMRKCRF
                     VRSAVGEANVVAEMQRVKSTLGGEGNGGVIDARIPSFGRDALAGVAHLVALLGNEKKS
                     LSQHVATLPQLVMVKEVRRGVSNETMAAAVKRFARAFSAAKLDRRDGYHFSGVFDDGT
                     GYWLHLRASNTEPVIRIIAEAASQAQIDALFAALAL"
     gene            1111..1611
                     /locus_tag="PLF85_16700"
     CDS             1111..1611
                     /locus_tag="PLF85_16700"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE21359.1"
                     /translation="MKVLYRDKEIAFTLVKTRFGDKPLLQYPHAAGILPVEQIRSGKK
                     FLWLVEQYRLGSGARSWEIPAGKKKNNETILQCAKRELAEEAGFAARHWKKHLQYHPA
                     ISFSTEVLHLFTARSLSEATAEHDDDEVFSNKKAFSQAELNRMVRNHRITDSKTLLAL
                     WIFEKL"
     gene            1717..>2097
                     /locus_tag="PLF85_16705"
     CDS             1717..>2097
                     /locus_tag="PLF85_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE21360.1"
                     /translation="MRIAVIGAGSFGTALAKISAENGHDVIIWSHSQDTAEAIRQNHE
                     NTIYLKGISLPKSIRVTTDFDEAVAHAELLVSATPTQAIREVYSKAGRAQNIKVPVVV
                     ASKGIEKKTHLLVCDVFTEIFGPAK"
BASE COUNT          487 a          563 c          611 g          436 t
ORIGIN      
        1 gacgccgacg atcaaggctt acgtgaagga gaaaaaactt gctggcggcc tcatggtcag
       61 cgcctctcac aatcctgcag aatacaatgc gctgaagttc atcaagaaag atggcttctt
      121 ctttgacgct gatgataata gccgttttgt ccgcgcgctc gaggcaaaag ctgattgggg
      181 cacttaccgc aagcagggaa aaattatcga ggcacacgcg caggcgattg atgtgcacgt
      241 aaaatctata ctccgggttg tgaagaagcc ccgccgcagg ctcagggtgg tcattgaccc
      301 tgtgggttct tgcggcggtg aaattgcgct ggcgttgctt gcgcgtcttg gtgcgcaggt
      361 gacagctatc caccttgaag aagtaccgga atttccgcgt ccgcccgagc ccgttgccgc
      421 agcgctgaag aagctggcag cggccgttaa aaaacacaaa gccgatatcg gctttgcttt
      481 tgatcccgat gccgacaggc tttcgctcgt cgacgagaag gggcgtgcta tcggcgaaga
      541 gtatacgcta ccgctggcaa ttctctctgc cgcgcgtcct ggcagtacga tcgtggcaaa
      601 cctttctact tcgatgctga ccgagcgcgc ggcgctcatg cgcaagtgcc gtttcgtgcg
      661 ctctgcggtg ggcgaggcaa atgtcgtcgc cgaaatgcag cgggtaaagt cgacactcgg
      721 tggcgaaggt aacggcggtg tcatcgatgc gcgcattccg tcattcggcc gcgatgcgct
      781 tgcgggggtt gcgcacctgg tagccctgct tggcaacgag aagaaatccc tatcgcagca
      841 cgtggcgacg ttgcctcagc tggtgatggt gaaagaggtc aggcggggag tcagcaatga
      901 aaccatggcg gcggcagtca aaagattcgc ccgcgctttt tctgcggcga aactcgacag
      961 gcgcgatggc taccacttca gcggcgtctt tgacgatggt acgggctact ggctgcacct
     1021 gcgtgcatct aacacagaac cggtcatccg catcatcgcc gaggcagcaa gccaggcaca
     1081 gattgacgcg ctttttgctg cgcttgcttt atgaaggttc tttaccggga caaagaaatt
     1141 gccttcacgc tcgtaaagac gcgttttggc gataagccgt tgctgcaata ccctcatgcc
     1201 gcgggcatct tgccggttga gcagatccgc tccggcaaga agtttctctg gctggttgag
     1261 cagtaccggc ttggttcggg ggcgagatcg tgggaaattc ccgcaggcaa aaagaaaaat
     1321 aacgaaacaa tcctgcagtg cgccaaacgg gaactggccg aagaggccgg ttttgcggcg
     1381 aggcactgga aaaaacacct gcagtatcat ccggcaatca gcttttcgac agaagtattg
     1441 cacctcttca cggcgcgcag cctgagcgaa gccactgcgg aacacgacga cgatgaggta
     1501 ttttccaaca agaaggcgtt cagccaggca gaactcaacc gcatggtacg caaccaccgc
     1561 attaccgata gcaaaacgct gttggcgctg tggattttcg agaagctcta gcaggttccg
     1621 gaacctgctt ttggctccat tttcaggagc ctgctggaat cacacccccg ggcaaataaa
     1681 cttgccgtcg taagcggcgc gcggcaattg cggactatgc gcattgcagt catcggtgcc
     1741 ggttcattcg gtacagccct cgccaaaatc tccgcagaaa acggtcatga cgttattatc
     1801 tggagccact cgcaggatac tgctgaagcg atcaggcaga accatgagaa cacgatttac
     1861 ctcaagggta tttccctgcc caaaagcata cgcgtcacga cagattttga tgaagcggta
     1921 gcgcatgcag agctcctggt cagtgcgact ccgacgcagg ccatacgcga ggtctacagt
     1981 aaagcgggcc gggcgcagaa tatcaaggta ccggtcgtcg tcgcctctaa gggtattgaa
     2041 aagaaaacac atctcctggt atgcgatgtg tttaccgaaa tattcgggcc ggcaaag
//
LOCUS       DAOQJF010000471         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_41987_length_2097_cov_2.626836, whole genome shotgun sequence.
ACCESSION   DAOQJF010000471 DAOQJF010000000
VERSION     DAOQJF010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41987_length_2097_cov_2.626836"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLF85_16710"
     CDS             complement(<1..241)
                     /locus_tag="PLF85_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE21361.1"
                     /translation="MTRDEVLRNVFGYETFRLDQQRIIDNVLAKRDTLVIMPTGGGKS
                     LCYQIPALIFEGLTVVVSPLISLMQDQVSQLNQLGV"
     gene            328..723
                     /locus_tag="PLF85_16715"
     CDS             328..723
                     /locus_tag="PLF85_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21362.1"
                     /translation="MRNIKIVHTVILFFSLSFVPVVIAAPQANPAGKRICREAPGPED
                     TLQFKLTLVQDGRLKGSGSFSGARGILYFKNGSWRLKEGRIYAELILVGSMSSGTKME
                     KVRREVKFDFMLSELLSSQGCFEPKIGNP"
     gene            complement(775..1593)
                     /locus_tag="PLF85_16720"
     CDS             complement(775..1593)
                     /locus_tag="PLF85_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21363.1"
                     /translation="MEYLKLGATPLIFLENPLLKNLLAIEYRKTTKSTTITANQKLYY
                     FPYTTYAGLTAQDSDIGLIFYEQKAAGSKLSYTIKERDWLFTIGIFALRLGYFDLSYS
                     KPYQMDADIYQGNTLIDQRIYLFEGQATGRGLMVGLQNLYFPNWDTKRFYFTTPDLLA
                     DGFFWGLKEAGFYWGTGSIRLQNNLDLAQKYRAFYQNETGHEPSVNFLRQVIHFVIGY
                     KFNRHFIAFVEYRYSNYSLALRDSYSDDTFNYFLSHAINRDTVQQFAINLTVGF"
BASE COUNT          501 a          442 c          571 g          583 t
ORIGIN      
        1 tgacgccgag ctggttcagt tggctcacct ggtcttgcat cagcgagatc agcggtgaaa
       61 cgacgacggt caggccttcg aagatgagcg cgggtatctg gtagcagagg gattttcccc
      121 cgcctgttgg catgatgacc agggtgtcgc gtttcgccag tacattatca atgatgcgtt
      181 gctggtcgag gcgaaatgtt tcgtagccaa agacatttcg cagaacctcg tctctggtca
      241 ttaattaata gagtcatttt tttcggtttc gtcaagcatt tctgtaaggg gtttacgcgg
      301 tcgttcgtag cggaatagag tcgatccatg cgcaacatca aaattgtcca caccgttatc
      361 ttattcttct cgctgtcatt tgttccagtc gtgattgccg cccctcaggc gaatcctgca
      421 gggaaacgta tttgccgtga agcgccgggg cctgaagata cgctgcagtt caaattgact
      481 cttgtacaag atgggcgctt aaaaggcagc ggttcattca gtggggcacg tggaattctt
      541 tattttaaga atggcagctg gcgcttgaaa gaaggccgca tttatgcgga gttaattctt
      601 gtgggttcga tgagttcggg tacaaagatg gaaaaggtcc gtcgtgaagt gaagtttgac
      661 tttatgctca gtgagctgct ttccagtcag ggttgctttg agccgaaaat cggcaatccg
      721 tgattggtgc ttgcgctttt gaacagcgac gcagcaatgc ggtgctcgac aatttcagaa
      781 gccgacggtc aggttgatcg caaactgctg gacggtatct ctgtttatgg catggctcag
      841 gaaataattg aaggtatcat cgctataact gtctcttaat gccaatgaat aattgctgta
      901 gcgatactcg acaaatgcaa tgaaatggcg gttaaacttg tagccgataa caaagtggat
      961 aacttgtcgc agaaaattta ccgacggttc atgaccggtt tcgttctgat agaatgcccg
     1021 gtatttctgg gcgagatcga gattattctg taggcgaata ctgccggtac cccaataaaa
     1081 acccgcttct ttcaagcccc agaaaaaacc gtcggcaagc aagtctggtg tagtaaaata
     1141 gaagcgttta gtatcccagt taggaaaata cagattttgc agacccacca taagcccgcg
     1201 acccgttgcc tgcccttcga aaaggtaaat ccgttggtcg atcagggtgt taccctggta
     1261 tatatcggcg tccatctggt agggtttgga ataactcaga tcaaaataac ccaaccgcaa
     1321 cgcaaatatg ccgatggtaa aaagccaatc acgttccttg atggtatacg agagctttga
     1381 gccggcggct ttctgttcgt agaaaattaa accgatgtca gaatcctgcg cggtgaggcc
     1441 cgcataggta gtatatggaa aatagtatag tttctggttt gctgtaatcg tcgtcgattt
     1501 tgtggtcttg cggtactcga tggcgagcag gtttttcaac agcggattct ccagaaaaat
     1561 gagcggcgtt gctccgagtt tcagatattc cattaacgaa ccgctggcgg tgttatcaat
     1621 gatcggaatc tcgcggaata ggtctttggg ttgaggtggt agggccgtca ggtaagagaa
     1681 atccagtccg aggacattga tgtcaacttc gtatgtggga aaaaaagtac tctggtgttg
     1741 tatggcgggg ctgaaatgaa aatcccaatt tgtgaatgcg attctggaat tgaccgtaat
     1801 attatttgtc agatggcttt ttttgtccgc ttcgttttca aactttcgtt gtttatcaac
     1861 aggggcttca acatctttgc actgtgtgca gttgtcgacg agctcagtcg tgtttgcttc
     1921 agatttgtta ccgcgggttt ctggcgattc cgagtgctct gtccgaggct gagaagaacc
     1981 gttacttcgg ctgggcccag gatcgtacga cggcctcgac ggagagggtg gtgaataaga
     2041 tggaggagca ctcggtgtgc tcttcgggct gcgctgcgtc tggttgcgca tcgtttt
//
LOCUS       DAOQJF010000472         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_42277_length_2090_cov_2.775430, whole genome shotgun sequence.
ACCESSION   DAOQJF010000472 DAOQJF010000000
VERSION     DAOQJF010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42277_length_2090_cov_2.775430"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..497)
                     /locus_tag="PLF85_16725"
     CDS             complement(147..497)
                     /locus_tag="PLF85_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21364.1"
                     /translation="MKEKKHGHDFNPIPLPARFRQLLLLGAVFCFAAPNGYFLYRYMK
                     EPQLMDALLANPLGVCFLSETALLVLLFLVAVWYLKQSAIKVLYYALAAFVGGLAFAL
                     PLFLWFNSYDQKKE"
     gene            complement(494..1306)
                     /locus_tag="PLF85_16730"
     CDS             complement(494..1306)
                     /locus_tag="PLF85_16730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE21365.1"
                     /translation="MQVRKPTFDFSGVKRYYFAENAWATHTMNALHVIVPIGELFFVR
                     AVRGLAAEAPPAQKDGIKKFIGQESVHDQVHRQFWQTLREQGLPVDSFAEFFRSTAID
                     TMEPIVEKLLGKKFLLSATVGFEHYTAALADIAFAPGSRLLSSMQKDVADMMRWHAAE
                     EIEHKSVAFRQLQSIDDSYLLRAAGMVLASSMLTLYTVIGTAWFIAGDRELTLARLIK
                     DLPQLPQIARGLVTGVIRHNAEYFMPGFDPDTKNNYPRAEAALRELGLAIAS"
     gene            1454..>2090
                     /locus_tag="PLF85_16735"
     CDS             1454..>2090
                     /locus_tag="PLF85_16735"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE21366.1"
                     /translation="MHAPIANYCSSAAAGGLELNVVRMTQWLRESGYPVTLVARPDSE
                     ISRRAKQLKLPRPAGMPESGKISIWRLSRILRAGNYHTLFVHTSKDLRCAVLARLLSL
                     RATRLVFVQHMQLGVRKKDPIHSWFFRRLSAWIAPLPYMVRQVAQMTRIDRRRIHEIP
                     FAAELEALAEKPCQTSARLRLGLPAEGIFAGVIGRFDRQKNQALLLRASAAL"
BASE COUNT          504 a          571 c          579 g          436 t
ORIGIN      
        1 cgcgtgacga gcgtgttgca gttcaggcga aacggtgaaa tcgaattttg cgaacggact
       61 tttcggcgcg gacagcctcg cggcgatcag agatttgtat tcaaagtgca gaactgagca
      121 aacggtctgc agagattgag cgagtatcac tccttctttt ggtcgtaact attgaaccag
      181 agaaagagcg gtagcgcgaa cgccaggccg ccaacaaacg cggcaagcgc atagtagagc
      241 accttgattg ccgattgctt caaataccag accgcaacaa gaaacagcag caccagcagg
      301 gcagtttcgc ttaagaagca tacgccgaga ggatttgcca gcagcgcatc catcagctga
      361 ggttctttca tgtagcggta caggaaataa ccgttagggg cagcgaaaca aaataccgca
      421 cccagaagca acaactgccg aaaacgcgcg ggtagcggaa tcgggttgaa atcgtgtccg
      481 tgttttttct ctttcatgaa gcgattgcaa ggccgagctc acgcagcgca gcttcagccc
      541 gggggtaatt gttttttgtg tcggggtcaa aaccgggcat gaagtactct gcgttgtgcc
      601 tgatgacacc ggtcacgagc ccgcgtgcaa tctgcggcag ctgtggcaag tctttgatca
      661 gccgggcaag cgtcagctcg cggtcaccgg cgatgaacca ggcggtgccg ataaccgtgt
      721 acaaggtcag catcgaagaa gccagcacca tgccggcggc acgcagcagg tagctgtcgt
      781 cgatcgactg cagttgcctg aaggcgaccg acttgtgctc aatttcttcg gcggcgtgcc
      841 agcgcatcat gtcggcgaca tctttctgca tcgacgacaa caaccgcgaa ccgggcgcaa
      901 aggcaatatc ggcaagggcg gcggtgtagt gctcaaagcc gactgttgcc gaaagcagga
      961 attttttacc caaaagtttt tcgacgatgg gctccatagt gtctatggcc gttgaacgaa
     1021 agaactccgc gaagctatcg actggcaagc cctgctcgcg caaggtctgc cagaactgcc
     1081 ggtgaacctg atcgtgcaca ctttcctggc cgataaactt cttaatcccg tctttttgcg
     1141 ctggcggagc ttctgctgca aggccgcgca cggcgcgaac gaagaacaat tcaccgatcg
     1201 gtacgatgac atgcagcgca ttcatggtgt gcgttgccca ggcattctca gcaaaatagt
     1261 aacgcttgac tccggaaaaa tcgaaggtcg gctttcggac ttgcatagac ggatattatt
     1321 ctaatgacac aagtgtcaat aaatatctga acaattgttc atgttggcaa ccaacaaaaa
     1381 ctagctcttt gtcctatatt tggcatagat tgtgctggaa ccatagggag aattcccggg
     1441 tctatccata acgatgcatg caccgatcgc caactactgc tcgtctgctg ccgcaggcgg
     1501 ccttgaactc aatgtcgtgc gcatgaccca atggctgcgc gaaagtggtt atcctgtaac
     1561 gctcgtcgca agacccgatt cggaaatcag ccgccgggcg aaacagctga aactcccccg
     1621 gccggccggc atgccagaaa gcggaaaaat ttcgatctgg cgcctgagcc gcattctgcg
     1681 tgcaggtaat taccacacac tgtttgtgca taccagcaaa gacctgagat gcgcggtact
     1741 ggcacgcctg ctctctttgc gcgccacccg gctggtattc gtgcaacaca tgcaacttgg
     1801 cgtcagaaag aaagacccga ttcacagctg gttctttcgc cggctctccg cctggatagc
     1861 tcccctgccc tacatggtga ggcaggtggc acagatgacg cgcatcgatc gacgacgcat
     1921 ccatgaaata ccgtttgctg cggagcttga agcgctggca gaaaaaccgt gccagacaag
     1981 cgcccgtctg cgcctggggt tgcccgccga aggaattttt gccggtgtga tcggtcgctt
     2041 cgaccggcaa aagaaccagg ctctgctact gcgcgcctcg gcggcgctta
//
LOCUS       DAOQJF010000473         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_42857_length_2076_cov_3.697180, whole genome shotgun sequence.
ACCESSION   DAOQJF010000473 DAOQJF010000000
VERSION     DAOQJF010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42857_length_2076_cov_3.697180"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLF85_16740"
     CDS             <1..819
                     /locus_tag="PLF85_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNE21367.1"
                     /translation="VRGLLERETLARVANEADHLRSDTAWACAAPLHYASVEDGEIYQ
                     TSAKSPKGDIVQALLYFEAQLRDKETPHERRVLALKWLVHLIGDLHQPLHVGRSCDRG
                     GNSVEVLWFDKKTNLHKVWDSEFINDEELSYTEYADFLGKDQAGSSGLAAGSYSDWAD
                     EAPIVRAELYTCHGKDGCCAAGKNCRDDTRAFDSCGDTQAFVPRLGYAYAEKNRKLME
                     RQLYRAGLRLAAVLNAIANGAPSASEKLARQLAAERKGKPDTVGQCINSATQQR"
     gene            823..1860
                     /gene="mnmH"
                     /locus_tag="PLF85_16745"
     CDS             823..1860
                     /gene="mnmH"
                     /locus_tag="PLF85_16745"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNE21368.1"
                     /translation="MANRLDIRAILAQQHEIPLIDVRSEGEFAQGHIPGAISLPLFNN
                     AERAEIGTLYKQQGQQPAILRGLGIVGPKMQQLAEAGLAHAREGRIAVHCWRGGMRSA
                     SVAWLFERVGLQVDTLIGGYKAFRRHCYELFAAPWRMTVIGGKTGTRKTELLHQAKER
                     GLQIVDLEAYANHRGSAFGYMASKNQAAEARDGQPTQEHFENLLGFDLARCDLAKPLL
                     VEDESRLIGRLHIPDAFWAQVRAAQVLVIELPLEARVNYLTEGYDFPSEKIRTSLTAI
                     RKRLGDERFGRAVAALEEGRMDEVCRITLDYYDRAYTFGLSKRDPSTVKVIAGEQALE
                     EIINHLRTDNA"
     gene            1873..>2076
                     /locus_tag="PLF85_16750"
     CDS             1873..>2076
                     /locus_tag="PLF85_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNE21369.1"
                     /translation="MSVRLTQYSHGAGCGCKISPAVLGDILKDNMVFPAANLIVGNSD
                     RDDAAAYLLGEGQVLLSTTDFFMP"
BASE COUNT          441 a          600 c          631 g          404 t
ORIGIN      
        1 gtgcggggtc tgcttgagcg cgagaccctg gcgcgcgtgg ccaatgaagc agatcacctg
       61 cgctccgaca ccgcctgggc atgtgctgcg ccactgcact atgccagcgt cgaagatggc
      121 gaaatctacc aaacttcggc aaaaagcccc aaaggtgata ttgtgcaggc gttgctctac
      181 tttgaggcgc agctgcgtga caaagaaact ccgcatgagc ggcgtgtact ggcgctcaag
      241 tggttggttc acctgatcgg cgatctgcac cagccgttgc atgttggccg cagctgtgac
      301 cgcggtggca actcggtcga agtgctgtgg ttcgacaaaa aaaccaacct gcacaaagtg
      361 tgggattccg aattcatcaa cgatgaggag ctgagctaca ccgagtatgc cgatttcctg
      421 ggtaaggatc aggcaggctc ttcgggtctt gctgccggca gctattctga ctgggctgac
      481 gaagcgccca tcgtgcgagc cgagctgtac acctgccacg gcaaagacgg ctgctgcgct
      541 gcaggcaaga actgccgcga cgacaccagg gcatttgaca gctgtggcga tacacaggca
      601 ttcgtgccgc gccttggtta tgcttatgca gaaaaaaacc gcaagctgat ggagcgccag
      661 ctgtaccgtg ccgggctgag gcttgcggcc gtgctcaatg cgattgccaa tggcgcgcct
      721 tctgcctcag aaaaactcgc gcgtcagctt gctgctgagc gcaaaggtaa acctgacacg
      781 gtcggccagt gcataaactc ggcgacgcag cagcgctaac gcatggcgaa caggctcgac
      841 atccgcgcca tactggcgca acaacatgaa atcccactga tcgatgtcag gtctgaaggt
      901 gagtttgcgc aggggcatat tcccggcgca atctcgctgc cactcttcaa caatgccgag
      961 cgcgccgaga tcggtactct ctataagcag caagggcagc agccggcgat tctgcgcggc
     1021 ctcggtatcg tggggccgaa gatgcagcag ctcgctgagg caggccttgc tcacgcgcgt
     1081 gaaggccgca ttgcagtgca ttgctggcgt ggcggtatgc gctccgcctc agtggcctgg
     1141 ctttttgaac gagtaggatt gcaggtcgat acgctcatcg gtggttacaa ggcgtttcgc
     1201 cgccactgct acgaactttt cgccgcgccg tggcgaatga ccgtgattgg cggtaagaca
     1261 gggacgcgca agactgaact gctgcaccag gcaaaagaac gcggcttaca gattgtcgac
     1321 ctcgaagcgt acgccaacca ccgcggttcg gcgtttggtt acatggcttc aaagaaccag
     1381 gcagccgaag cacgcgatgg ccagccgacg caggagcact tcgagaacct tttgggcttc
     1441 gacctcgcgc gctgtgatct cgccaagccg ctgctggtcg aagacgaaag ccggctgatt
     1501 ggccgcctgc atatacccga cgccttctgg gcgcaggtac gcgcggcgca ggttctcgtc
     1561 atcgaactgc cgctggaagc ccgagtgaac taccttacag aagggtatga cttccccagt
     1621 gaaaaaatcc gcacgagtct gacggccatt cgtaagcgtc tcggtgacga acgcttcggg
     1681 cgcgcggtgg ctgcgctgga agagggccgc atggatgagg tctgccgcat cacgctcgac
     1741 tactacgacc gggcgtacac tttcggcctg tcgaagcgcg atccctcaac ggtaaaggtg
     1801 attgcgggtg agcaggcgct ggaagaaatc ataaatcatt tgcggactga caacgcctga
     1861 caatactaac ccatgtctgt ccgtttgacc cagtatagcc acggtgccgg ctgcggctgc
     1921 aagatttcgc ccgcggtgct cggcgacata ctgaaagaca acatggtctt tcccgcggcg
     1981 aacctcatcg ttggcaattc tgaccgtgac gatgcagcgg cgtacctgct cggcgagggc
     2041 caggtgttat tgtcgaccac tgacttcttc atgccg
//
LOCUS       DAOQJF010000474         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_43700_length_2055_cov_4.750000, whole genome shotgun sequence.
ACCESSION   DAOQJF010000474 DAOQJF010000000
VERSION     DAOQJF010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43700_length_2055_cov_4.750000"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1941)
                     /locus_tag="PLF85_16755"
     CDS             complement(169..1941)
                     /locus_tag="PLF85_16755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE21370.1"
                     /translation="MTRFKLLNSLAFRNLVRQRRRNMLLGVALAFGTMILIVANAFSA
                     GITDTLLNKIVVQMTGHIELAVIERARAQNVLIRDREQYLQLLKQNLPDIREIAENVA
                     VFSRAIGVGKGENVIVLPVDLGDAADPAQSTDAEYLVSRATEGNVKDLLSHEYENPVA
                     IFADKAKALNVKLLDTLKMRLKTVSGQEQSARFTVVMLLKSESMFESGALFLPLQKLK
                     ELRGIRANETGSLQVTFKKFDSPRSVIRLADKIHAQLKPGIAAIGGELVSGKKVLPVT
                     AAGLLQEFEKSAVVQRSLALESGKWFRDRDTDQVVLSERAAEELGLSEGGAVTLRYRS
                     IHGAEPVEQTFKISAVVSAKENLPGIDAWLPEAGFYRVYLGDMPQQRVLTGAEKLSAE
                     WKVVLAPEFRVLPRTATGDDLMKKNQELRKTNFFGPAIDVRTMYESASQVLELEGALN
                     LITLIAVLILFLIILTGVVNTLRMTIRERTREIGTMRAIGVRSTDVRNVFLLETFQLA
                     AFSVLAGLLLAFIVMTVLSSFRIETQSVLGIFLSKKKLYFLPQFGDILRSIVMILVIS
                     TATAYFPSRRAAKLDAAEALRHYE"
BASE COUNT          455 a          622 c          543 g          435 t
ORIGIN      
        1 acgccctgtc caaccttctg ctgcgtcaat gcgaccttgg cagaaaaatc gctgacctgc
       61 gacatacgtt tatcgatctc ttgcagaatc ttgttcacgc ctgtggcatg aataagattc
      121 tgtgaaacta gtgcgaatac aagcagccat tttttcattg taaacccctt attcataatg
      181 cctcaatgcc tcggccgcgt cgagtttggc ggcgcggcgt gaaggaaagt atgcggtcgc
      241 tgtcgaaatt accagtatca tcacgatact gcgcaaaata tcgccaaact gtggcaaaaa
      301 gtagagcttc ttcttgctca gaaagatacc gagcacgctc tgtgtttcga tacgaaatga
      361 cgagagaacc gtcatgacga tgaaggcaag cagaagcccg gccagcaccg agaaggcggc
      421 gagctgaaag gtctcaagca aaaatacatt gcgcacgtct gtgctacgca caccgatggc
      481 ccgcatcgtt ccgatttccc gtgtgcgctc gcgtattgtc atgcgcagcg tgttaacgac
      541 acccgtcaga ataatgagaa aaagtatcag cacggcgatc agcgtgatca ggttcaacgc
      601 tccttcgagc tcgagcacct ggctggcgct ctcgtacatc gtgcgcacgt caatcgccgg
      661 cccgaagaaa tttgtcttac gcagctcctg gttcttcttc atcaggtcgt cgcccgtcgc
      721 ggtgcgcggc aaaaccctga actcgggcgc caggacgacc ttccattcgg cgctaagctt
      781 ctcggcgccg gtaagcacac gctgctgcgg catgtcgccc agatagacac ggtagaagcc
      841 agcctcaggg agccacgcat cgataccggg aaggttctcc tttgccgaca cgactgcaga
      901 gatcttgaag gtctgctcga cgggttctgc gccatgtatc gaccggtagc gcagggttac
      961 cgcgccgcct tcagagagac ccagctcttc ggccgcgcgc tcactcagca caacctggtc
     1021 agtatcgcgg tcacgaaacc atttgcccga ttcgagcgcg agcgaacgct gcaccacggc
     1081 agacttctca aattcctgca gcagccctgc cgccgtcacc ggcagcacct tcttgccgct
     1141 gaccagctca ccgccgatcg ccgcaatacc cggcttcagc tgcgcatgga tcttgtcggc
     1201 gaggcgaatc accgaacgcg gtgaatcaaa ctttttgaat gtgacctgca gcgagccggt
     1261 ttcgtttgcg cgaatgccgc gcagctcttt tagtttttgc aggggcaaaa acaacgcgcc
     1321 gctctcgaac atactctctg acttgagcag catcaccacg gtaaaacgcg cgctctgctc
     1381 ctgcccgctc acagtcttga gccgcatctt gagcgtatcg agcagcttga cattcaatgc
     1441 cttcgccttg tcggcgaaga tcgccaccgg gttttcgtac tcgtgagaga gcaggtcttt
     1501 gacattacct tcggtcgcac ggctcaccag gtattcggca tcggttgatt gcgccgggtc
     1561 ggcagcatca cccagatcaa ctggcaacac gatcacattc tcgcccttgc cgacgccgat
     1621 cgcgcgcgag aacaccgcga cgttctcggc aatctcgcgg atatcgggca ggttttgttt
     1681 gagcagctgc aggtactgct cgcggtcgcg gatcagcacg ttctgcgccc gtgcacgctc
     1741 gatcaccgcg agctcgatgt gcccggtcat ctgcaccacg atcttgttga gcagcgtatc
     1801 ggtaatacct gccgagaaag cattggcgac aatcaggatc attgtgccaa acgcgagcgc
     1861 tacgcccaga agcatattgc gcctgcgctg gcgcaccagg ttacgaaacg cgagcgaatt
     1921 taggagttta aatctggtca tgtcccctcc taatccctcg aaatggcatc gagcggtgta
     1981 atgcgccgcg cgacaataat cggataaatc gctgccatga tggtaaccca ggcgagctcc
     2041 gccacgccga agaca
//
LOCUS       DAOQJF010000475         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_44238_length_2042_cov_4.818822, whole genome shotgun sequence.
ACCESSION   DAOQJF010000475 DAOQJF010000000
VERSION     DAOQJF010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44238_length_2042_cov_4.818822"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="PLF85_16760"
     CDS             <1..1833
                     /locus_tag="PLF85_16760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNE21371.1"
                     /translation="ILAQVGDAPQPLARRVSLLQGRLLLLTAVAAPARAEHWQKHADK
                     FSDAESLYQAAHGLDMAGDKRGAGQLAWRALEKPEADFPFAQSGLLLRNLLGQAIYTQ
                     PSALQRIRLMEALRVAKDRASAAKLWQTLSRERLSGKEQLLFTQYAARLLTDKGNFAE
                     LSRTVAGAGAEFLAADNEKPALDICERLLKKKQFALVRNLFPLEPATKAAYQCRLRLA
                     QRSSDYSPAARSLAARYIAEFDAESTLAERIFLRSCLPGVGTHHENWDIACLEELRQV
                     SVQKNLGAGARYFLARHYDSAGDTAKVRELLTELALNYSDDYYFYRLMERPISAQKTW
                     AQSYRSDGTRLGYLLEVLLTGDVARARDLPQMAELSKFESEIAQLAGRLDDDRVMALL
                     LLAADSRDEMRELLRSEEKPGIYKLLAAMGVVAQKPDMALYGAKMLVRDSKLRPFLFE
                     IPGTLRAMLYPQAYGAYVETYAAKNQLERAEVFALIRQESQFFAGAISVAKAQGLMQI
                     LPSTAELLAAKEGLGDYNLLKPEDNIRLGTSFMRDIRKAYAADFTGLAIAYNAGPGRL
                     QQWRKKYSNDDDIFVEEIPFQETYHYVRVLLADRARYRALLSGK"
     gene            1830..>2042
                     /locus_tag="PLF85_16765"
     CDS             1830..>2042
                     /locus_tag="PLF85_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21372.1"
                     /translation="MKPRTLVDVARRSPLFKGISEAQCRRVLKNFHLHRLDKGDVLFR
                     ADQKSRVMYFVVDGKLRVEKLTDQQQL"
BASE COUNT          440 a          601 c          598 g          403 t
ORIGIN      
        1 atactcgccc aggtaggtga tgctccgcag ccgctcgcgc ggcgtgtatc tctgctgcag
       61 ggccgtcttt tgcttctgac tgcggtggct gctccggcgc gcgccgaaca ctggcaaaag
      121 catgccgaca aattcagcga tgccgaatca ctctaccagg cggcgcatgg cctcgatatg
      181 gccggtgaca agcggggggc cgggcagctg gcgtggcgag cgctcgaaaa gcccgaagcg
      241 gactttcctt ttgcccagag tggcctgctg ctgcgtaatc tgctggggca ggccatctat
      301 acacaaccgt cagccttgca gcgcatccgc ctgatggagg ccctgcgcgt ggcgaaggac
      361 agggcaagcg cagcgaaact ctggcagaca ctcagtcgcg agcggctctc gggaaaagaa
      421 cagttactct tcacgcagta tgctgcgcgt ctgctcaccg acaagggcaa tttcgctgaa
      481 ctcagccgca ccgtggccgg cgcaggggcg gagtttttgg ctgctgacaa cgaaaaaccc
      541 gcgctggata tctgcgagcg cttactcaag aagaagcagt ttgcccttgt acgcaacctg
      601 ttcccgcttg aacctgcgac caaagcagcc taccagtgcc gcctgcgtct tgcacagcgc
      661 agctccgact attcgccggc agcccgctcg cttgccgccc ggtacatagc agaatttgat
      721 gccgaatcaa ctctcgccga gcgcatcttc ctgcgctcat gcctgccagg agtcggcacg
      781 caccacgaaa actgggatat cgcctgcctc gaagagctgc ggcaggtatc ggtgcagaag
      841 aatctcggtg cgggtgcgcg ctactttctc gcgcggcact acgatagcgc gggcgatacg
      901 gcaaaagtcc gtgaactgct gactgagctg gcgctgaact attccgatga ctattatttc
      961 taccgcctca tggagcggcc gatcagcgcg caaaaaacct gggcacagag ttatcgctcc
     1021 gacggtaccc gtcttggtta cctgctcgag gtactgctga caggcgacgt ggcgcgcgcg
     1081 cgtgacctgc cccagatggc agagctcagt aagtttgaaa gcgaaatcgc acagcttgca
     1141 ggacggctcg acgacgacag ggtgatggcg ttgttgctgc tcgctgcaga cagccgcgac
     1201 gaaatgcggg agcttctgcg ttctgaagaa aaacccggca tttacaaact gctcgctgca
     1261 atgggtgtgg tcgcgcagaa accggatatg gcgctctacg gtgccaagat gctggtacgc
     1321 gattcgaagc taaggccatt cctgtttgaa atacctggta ctctgcgtgc catgctctat
     1381 ccgcaggcgt atggtgctta cgtcgaaacg tatgcagcaa aaaaccagct cgagagagcc
     1441 gaagttttcg ctctcatccg ccaggaatcg cagttttttg cgggggcaat cagcgtggcc
     1501 aaagcacagg gtttgatgca gatactgcct tctaccgctg aactgctcgc ggccaaagaa
     1561 gggttaggcg actacaacct gctcaagcca gaagataata tccggctggg taccagcttc
     1621 atgcgcgaca tccgcaaagc ttatgctgcc gactttaccg gccttgctat tgcctacaat
     1681 gcagggcctg gccggctgca gcagtggcgc aagaaataca gcaacgacga cgatatcttc
     1741 gtcgaagaga taccgttcca ggaaacctac cattatgtgc gggtactgct ggccgaccgg
     1801 gcacgctacc gcgcgttgct ctccgggaaa tgaaaccgcg caccctcgtc gacgttgccc
     1861 gtcgcagccc gctctttaag ggtatcagcg aagcgcagtg caggcgtgtg ctgaaaaact
     1921 ttcacctgca ccggctggac aaaggcgacg tgttgtttcg tgccgaccag aaaagccgcg
     1981 tcatgtactt cgtcgtcgat ggcaagctgc gcgtcgaaaa gttaactgac cagcagcaat
     2041 tg
//
LOCUS       DAOQJF010000476         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_44765_length_2029_cov_5.034954, whole genome shotgun sequence.
ACCESSION   DAOQJF010000476 DAOQJF010000000
VERSION     DAOQJF010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44765_length_2029_cov_5.034954"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLF85_16770"
     CDS             <1..625
                     /locus_tag="PLF85_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21373.1"
                     /translation="CNNYDLLDRLESPGSSTNERFATNYYVFVSSWTTAGDMSNSPYP
                     ECSTSTGPARADCACTRAAIQRGFRRSASHVFRAWLSVSGVSDAKCRVQAQGNGCATN
                     FPAPWLNTQGQQVVANFNDFGLGGLANPIKFDEFGVDQGPNLVWTNTGSSGTALTADC
                     SGWTSNSAGFFGAIGNRTSGGPIWTAEVPDQACNTAQRMYCFASPAF"
     gene            738..1778
                     /locus_tag="PLF85_16775"
     CDS             738..1778
                     /locus_tag="PLF85_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE21374.1"
                     /translation="MIYRRLGTTNLDVSAICLGTMTWGTQNTEAEGHEQMDYALAQGV
                     NFFDTAEMYAVPPAAETYGKTESIIGTWFAKRKNRDKVILATKIAGNGLQWIRGGSSI
                     TGETVKLAVEESLRRLQTDYIDLYQLHWPNRGSYHFGQQWNYKIKNSTNEQELENFIE
                     VLSTLRALMTAGKIRHVGLSNETAWGTMKYLQLSEARDLPRVQSIQNEYSLLYRLHEP
                     DLMEISLREKVGLLAWSPLAAGMLTGKYANGARPQGSRWTLSKRFNQRDTPQAHAAVD
                     RYLAVAKKHGLDVAQMAIAFVLSRPFVTSAIIGATSMTQLRTNIAAAEISLSDEVHSE
                     IAAVRRDFTMPF"
     gene            complement(1812..>2029)
                     /locus_tag="PLF85_16780"
     CDS             complement(1812..>2029)
                     /locus_tag="PLF85_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21375.1"
                     /translation="AGFGTRIGALWVGVIMLSAMFMVHWQNGFFMNWYGAQKGEGIEF
                     FILAIGIAAALVISGGGHFSVDALIAG"
BASE COUNT          489 a          585 c          553 g          402 t
ORIGIN      
        1 gtgcaacaac tacgacctgc tcgacaggct ggagagtccc ggtagctcca ccaacgaaag
       61 atttgcgacg aactattatg tgttcgtcag ctcatggacg accgcgggag acatgagcaa
      121 ttcaccgtat cccgaatgta gcacatctac cggccctgcc cgcgcagact gcgcctgcac
      181 acgcgcagcg attcagcggg ggtttcgccg ctcggcttcg catgtctttc gcgcatggct
      241 ttcggtaagt ggcgtttcgg acgcgaaatg ccgcgttcag gcgcagggca atggctgcgc
      301 tacgaatttt ccggcaccct ggttgaatac tcaagggcaa caggtggtcg ccaacttcaa
      361 cgactttggg cttggagggc tggcgaatcc gataaaattt gatgaatttg gtgtcgacca
      421 gggtccgaac ctcgtgtgga ccaataccgg cagttccggg actgcactga ccgcagactg
      481 ctctggctgg acaagtaaca gcgcaggttt tttcggggcc atcggtaaca ggacctctgg
      541 ggggccgatc tggactgccg aagtaccaga ccaggcctgc aacacggcgc agcgcatgta
      601 ctgtttcgcc tccccggcct tctgacagtt tttccaccaa agccgtaagg gggcgctgat
      661 gccgggcggg caagcagtga tgccagcaga gtttttgcct tgccgctgct gttactcacc
      721 ggcatgagac gttatctatg atttaccgcc gcctcggaac taccaacctc gacgtcagcg
      781 ccatctgcct cggcaccatg acctggggca cgcagaacac agaagccgaa ggccatgagc
      841 aaatggatta cgcgctcgca cagggcgtaa attttttcga taccgctgaa atgtatgcgg
      901 tgccgccggc cgccgaaacg tacggcaaaa cagaaagcat catcggtacg tggtttgcca
      961 aacgcaagaa ccgcgacaag gtaattcttg cgacaaaaat agccggcaac ggcctgcagt
     1021 ggattcgcgg cggctcgtcg attaccggcg agacggtaaa actcgcagta gaagaatcgc
     1081 tccgccgcct gcagaccgac tacatcgacc tctaccagct gcactggccc aaccggggca
     1141 gctaccactt cggccagcag tggaactaca aaatcaaaaa cagcaccaat gaacaggaac
     1201 tcgaaaactt tatcgaagta ctttcaacgc tgagagcact catgactgcg ggcaaaattc
     1261 gccacgtggg attatcgaat gaaacagcct ggggcacgat gaagtacctg cagctttcag
     1321 aggcgcgaga tctgccccgt gtacagtcga tacagaacga gtacagcctg ctctaccgcc
     1381 tgcatgaacc tgatctgatg gaaatttctt tgcgcgaaaa ggttggtctg ctcgcctggt
     1441 cgccgctcgc tgcaggcatg ctgaccggca agtatgccaa tggcgcgcgc ccccagggca
     1501 gccgctggac actatcgaag cgcttcaacc agcgagatac accgcaggcg catgccgcag
     1561 ttgaccgcta cctggccgtg gcaaaaaaac atggattgga tgttgcccag atggcaatag
     1621 ccttcgtact ttcgcggcca tttgtgacga gcgcaattat cggtgcgacc agcatgacgc
     1681 agctcagaac caatattgct gctgcggaga tatcactttc tgacgaggtg catagtgaaa
     1741 tcgcggctgt gcgccgcgat tttactatgc cgttttaatc ctggcgcggc aaatgccgcg
     1801 ctacttataa tttaaccggc aataagtgca tcgactgaaa aatggccacc gccactgatc
     1861 accagcgcag ctgcaatacc gatggccagg atgaagaact cgatgccttc gcccttttgc
     1921 gcgccatacc agttcatgaa gaaaccgttc tgccagtgga ccatgaacat tgcagacagc
     1981 atgatgactc cgacccaaag cgcaccgatg cgtgtaccaa aacctgcaa
//
LOCUS       DAOQJF010000477         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_44981_length_2024_cov_4.652108, whole genome shotgun sequence.
ACCESSION   DAOQJF010000477 DAOQJF010000000
VERSION     DAOQJF010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44981_length_2024_cov_4.652108"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLF85_16785"
     CDS             <1..455
                     /locus_tag="PLF85_16785"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNE21376.1"
                     /translation="SLCLYARDNGIELPALQMLWYPMTHGDTEFPSRVQFGSDYYITR
                     DDMAWFSEQLLESQDHAKDIYFAPGLAENLSGLPSALILTAGYDVLRDEGQEYARQLA
                     AAGVRVEQVCYEDMFHGFISFAWGLTQGMKAIEQTAAFFRRATATPSS"
     gene            complement(433..918)
                     /locus_tag="PLF85_16790"
     CDS             complement(433..918)
                     /locus_tag="PLF85_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21377.1"
                     /translation="MRRLSFFLLLSAFQAGIMAETVDFVELAGKAFPADGSINREYGD
                     QLYAELYKLPEWHFLMTPASIRRKQPSVQVIDGKGWLLVFTDTGLLRKYAEQNKNLDA
                     KGNALFVSMAPDKALQFLKQYEKSPIGGVRFNEGADHGWFSPVRNLFIFPDYLKKKGL
                     L"
     gene            complement(1008..>2024)
                     /locus_tag="PLF85_16795"
     CDS             complement(1008..>2024)
                     /locus_tag="PLF85_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE21378.1"
                     /translation="VVLSAGLEMEFRSVIDSLRGRGLVIATLGFLVPMSAGIGVGYFY
                     GLDTMRMIFLGLCVSITALPVAVRILQKFQLLDSDIARYSVATAILNDVTALLALGVL
                     LNLPTQFSLGAVGVSILITASKLIVLALFILGFNWLVELLIHRGVRIPRWSEKLVEVV
                     GPEALFGILVVFVLAFGSVSEALGFHFVIGAFFGALLIDRKFFLASRFNELDHTLRSV
                     TDGFLGPVFFATIGLEFQAGKIQSVDFALAVLGVSIVSKLASGWAGARLIGLRPVDAL
                     GIGIILNGRGVMELVIASIAYHRGFIGQGLFSVLVLMGVVTTVITPILFQRWVLPRLR
                     PEGA"
BASE COUNT          519 a          580 c          547 g          378 t
ORIGIN      
        1 cgtcactctg cctctacgcg cgcgacaacg gtatcgagct accagcgctg cagatgctct
       61 ggtaccccat gactcatggc gatactgaat ttccttcgcg tgtgcagttc ggcagcgact
      121 attacatcac tcgcgacgac atggcatggt tttcagaaca actgctcgaa tcgcaagacc
      181 acgcaaaaga tatctacttt gcgccagggc tggccgaaaa cctgtcaggc ctgccctctg
      241 cattgatact cacggcgggc tacgatgtcc tgcgcgatga gggacaggag tatgcgcggc
      301 agctggctgc ggccggcgtg agggtcgagc aggtttgcta cgaagacatg ttccacggtt
      361 tcatttcgtt tgcctgggga ttaacccagg gtatgaaagc cattgaacag acggcggctt
      421 ttttccggcg tgctacagca accccttctt cttgaggtaa tcgggaaaaa taaacaggtt
      481 gcgcaccggc gagaaccagc catggtccgc accttcgttg aaccggacgc ctccaatcgg
      541 agatttttca tactgcttca gaaactgcag ggccttgtca ggcgccatgg atacgaatag
      601 cgcgttgccc tttgcgtcca ggttcttgtt ctgctcggcg tattttctca gcaggcccgt
      661 atcagtgaac accagcagcc agccctttcc atcgataacc tggaccgaag gctgcttgcg
      721 ccggatactc gccggcgtca tgagaaaatg ccactcaggc agtttataga gttcggcata
      781 gagctgatcg ccgtattccc ggttaatgct gccgtcagca ggaaaagctt ttcccgcaag
      841 ctccacgaaa tcaacggttt cagccataat gcctgcctga aatgcggaca gcaagaggaa
      901 gaagctcagt ctgcgcatgg tatacagttg atgtattcgg tgtcggtgtc aattcagaat
      961 cagcaaggcg gaggctgcaa gaattactcc gccgctcatg gcgatgttta tgcgccttct
     1021 ggccgcagcc gcggcaatac ccagcgctgg aagagaatcg gggtgatcac cgtggtgaca
     1081 acgcccatca ataccagcac cgagaagagc ccctgcccga tgaaaccacg gtgataggca
     1141 atcgaggcaa tgacaagctc cataacaccc cgcccgttca ggataatgcc aatgcccagt
     1201 gcatcaaccg gccgcaggcc aattaggcgg gcgcccgccc agcccgaggc aagcttggag
     1261 actatagaaa cacccagtac agccagggca aaatccacag actgtatttt gccggcctga
     1321 aactccaggc caatggttgc gaaaaaaacc gggcccagga aaccatcggt cacagagcgc
     1381 agggtatggt cgagctcgtt gaagcgggaa gccaggaaaa acttccggtc gatcagcagg
     1441 gcgccgaaga aggcgccgat cacaaaatgg aagccgagcg cctcgctcac agaaccgaac
     1501 gccagcacga agacaacgag gatgccaaag agggcctctg gcccaaccac ttcgaccagt
     1561 ttttctgacc agcggggaat ccgcaccccg cggtgaatca gcagctctac cagccagttg
     1621 aacccgagaa tgaaaagagc cagtacaatg agtttggaag ccgttataag tatagaaact
     1681 cccactgctc cgagtgagaa ctgcgtcggc agattgagca gtacaccgag cgcgagaagc
     1741 gccgtgacat cgttgagaat cgcagtcgcg acggagtagc gggcgatatc tgaatcaagc
     1801 agctggaatt tttgcagtat tcgcacggca acaggcagtg ctgtaatcga aacgcacaga
     1861 ccgagaaaaa tcatgcgcat ggtgtcgagc ccgtagaaat aacccacacc aattcccgca
     1921 gacatcggca ccaaaaagcc gagcgtcgcg atcaccagcc cccgaccacg cagtgagtca
     1981 atcaccgaac ggaattccat ctccagccct gctgacagaa cgac
//
LOCUS       DAOQJF010000478         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_45214_length_2019_cov_4.332485, whole genome shotgun sequence.
ACCESSION   DAOQJF010000478 DAOQJF010000000
VERSION     DAOQJF010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45214_length_2019_cov_4.332485"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PLF85_16800"
     CDS             complement(<1..1217)
                     /locus_tag="PLF85_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21379.1"
                     /translation="MRAIVLPFLLFFTVGGLAFAQNTATRPSFSPEEQERKIEILSEE
                     IEKLKAGKELFPAVKATGKYGLGPAASKVYDVQQGVSIGGYGELLYQRYDRTNQSGVD
                     VTNTSVDQSDFVRAILYFGYKFNDKMIFNSEIEIEHAKEVSLEFAYFDYLWRPWLSFR
                     AGKILLPMGLINELHEPTVYLGAKRPMTEQFIIPSTWSENGVGAFGTIGDFNYKAFVL
                     NGFDALGSANGGSVDGFNATSGLREGRQDGGEAKSVSFAGVARADYIGLSGLLAGISG
                     YYGRAGHNFRTPIDVWIADAHVDARYAGFLARTVLAYSRVGNVSDLYQQQQTGGTLTA
                     SSAAVGSDLYGGYFELGYDVLHLFGSDQELVAFGRFERMDTQFRQPAGFQRDGAADRT
                     TYTLGLRYKPIVQL"
     gene            1670..>2019
                     /locus_tag="PLF85_16805"
     CDS             1670..>2019
                     /locus_tag="PLF85_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21380.1"
                     /translation="MLRATWICCFFFLASLQVSPASQPSIVPGFVLPDRAGTFEIDQG
                     CSFRAPQHDAEYVQFTGTLTLGFRHEALDLRCNIHVNESSRLLRLVLAAPWLDRVMLL
                     PEGKGEPLLAGELVP"
BASE COUNT          450 a          560 c          558 g          451 t
ORIGIN      
        1 agttgcacaa tcggcttgta acgcaggccg agggtgtaag tggtgcggtc ggcggcgccg
       61 tcgcgctgaa agcctgccgg ctggcgaaat tgcgtgtcca tgcgctcaaa gcggccaaat
      121 gccacgagtt cctggtctga gccaaacagg tgcagcacat cgtaacccag ttcaaagtag
      181 ccgccgtaga ggtcagaacc gacagcagcc gatgacgcgg tgagcgtgcc gccggtctgc
      241 tgctgctggt aaagatcgct cacattgccg acgcgcgagt aggcaagtac cgtgcgcgcc
      301 agaaagcccg cataacgtgc atcgacgtgc gcatcggcaa tccagacatc gatcggggtt
      361 ctgaagttgt gaccggcacg gccgtagtaa cctgaaatgc ccgcgagcag gccgctgagg
      421 ccgatatagt cggcgcgcgc cacccccgca aagctgacag atttcgcttc gccgccgtcc
      481 tgccggcctt cgcgcaggcc gctcgtggcg ttgaagccgt cgacgctgcc gccgttcgcg
      541 ctgcccaaag cgtcaaagcc gttcagcacg aatgccttat agttgaagtc gccaatggta
      601 ccgaaagctc ccacaccgtt ctcagaccag gttgatggaa taataaactg ctcggtcatc
      661 gggcgtttag cgccgaggta gactgtcggc tcatgcagtt cgttgatgag ccccatgggt
      721 agcagaattt ttccggcgcg aaagctgagc cacggccgcc aaaggtagtc gaagtaggca
      781 aattcgagac tgacttcttt ggcgtgttcg atttcaattt ctgagttgaa gatcatctta
      841 tcattgaact tgtaaccgaa atacagaatc gcgcggacaa aatcggactg gtcgaccgat
      901 gtattggtga catcgacgcc gctctggttc gtacggtcgt agcgctggta gagcagttcg
      961 ccgtaaccgc cgatcgaaac gccctgctgc acatcataga ccttcgacgc cgccggaccg
     1021 aggccgtact ttccggtagc cttaactgcc ggaaaaagct ccttgccggc cttgagtttt
     1081 tctatttctt ctgacagtat ctcaattttt cgctcctgct cttcaggcga gaaggagggg
     1141 cgagtcgcgg tattctgggc aaaagccagt ccccctacag taaaaaagag tagaaaaggt
     1201 aagacgatag cgcgcatcca ggctccgtaa atagataatg agaataatta tcaattatat
     1261 ttagggcgtc aatcccaaaa cgggagacaa gctcggctac cgtcagggtc atcggcgaag
     1321 aagatagcct ggctgctatt ccgggatcgg ggcaggggga cgtgacaata ccttcagcaa
     1381 gatcagcgac cttaatctca agcaggtgct gctgctcgac gaaacggcac ttgatattga
     1441 acgcaggaca cagccttttc cggtacctcc accagaatac gtgcatgcgc tgcgccagcg
     1501 cggcgataat attatggtat ttcgcccgca gataagtttt actcttacag gcctgcgcta
     1561 aaaagcctgc gcacaatctt gttggggcgt ttttacactg agtaacagca agagctgccc
     1621 caaaattcgc ttggcaatgt gagctattca cggcactgct gccgctgtta tgctgcgggc
     1681 gacatggatc tgctgctttt ttttcctggc cagcctgcag gtttcacctg ccagtcaacc
     1741 atccattgtt ccaggctttg tgttacctga ccgtgcgggg acatttgaaa ttgaccaggg
     1801 ctgttccttc agagcgcctc agcacgacgc cgagtatgtt cagttcacag gaaccctgac
     1861 actcggtttt aggcacgaag ctctcgatct tcgctgtaac attcacgtca atgagtcttc
     1921 gcgcctgcta cggcttgtac ttgccgcacc ctggcttgat cgcgttatgc tgcttcccga
     1981 aggcaagggt gaaccgcttt tggcgggcga acttgtccc
//
LOCUS       DAOQJF010000479         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_45224_length_2019_cov_2.899695, whole genome shotgun sequence.
ACCESSION   DAOQJF010000479 DAOQJF010000000
VERSION     DAOQJF010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45224_length_2019_cov_2.899695"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLF85_16810"
     CDS             complement(<1..436)
                     /locus_tag="PLF85_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE21381.1"
                     /translation="MSDSAAVMHGIYLDHFSTTPVDPGVLDYYCQILKGNYGNPSSVD
                     HDFGSAAARLVKASREKIADLIACDPGEITFTSGATESISLFLQGFATAQSARLGRKL
                     KVITSPIEHPAVLENLQALQAAGLIALVTLDVDPQGRINLHQL"
     gene            complement(429..1271)
                     /locus_tag="PLF85_16815"
     CDS             complement(429..1271)
                     /locus_tag="PLF85_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNE21382.1"
                     /translation="MAKKSAAAVLKKPKDEQKAVRHILGISGGKDSAALAIYMRDKVP
                     NIEYFFCDTGEELAETYDFIDRLEARLGIKVQRLAATQGFRHFLEKYNDFLPSPQSRW
                     CTVQMKIKPLEDFIGDDEAVSYVAIRADEDRSGYVATKDTIQVVFPFKDAGITKADVE
                     RILDESGVGMPEYYKWRSRSGCYFCFFQRKIEWVRLAENHPALFEKAKSFEKVLPDGR
                     RYTWSQDESLDELIARKDQIIAEHEKRVKLQKQRKPNKTLAEVLEGVLDGEDQEEGCV
                     VCHV"
     gene            complement(1264..>2019)
                     /locus_tag="PLF85_16820"
     CDS             complement(1264..>2019)
                     /locus_tag="PLF85_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21383.1"
                     /translation="KEPDALIFQEIPAALGFDLSDKQNDTQAVRTLTKALTKSLHELQ
                     NAYLTLLEDCRSSLVDGLRIPKTTELRQYLGAMAGLLAGQVLDPVLRRFMNAAQDKDK
                     TDQQWLEGLIMVIADKPAEAWRDEDRHSFEANLATLAQKFLNLHQLAAKVQAAGNGKF
                     DARLVSITNPDGHEAREVIWVDAAEQKRAEKFKAELEALPVWKEAPDRIRQAVLADLL
                     QKEIALGPKTETNFAGEDKKPHIKIERKGNASG"
BASE COUNT          426 a          540 c          534 g          519 t
ORIGIN      
        1 ctaactggtg cagattgatg cggccttgcg ggtcaacatc tagagtcacg agagcaatta
       61 agccagcagc ttgcagcgct tgaagattct caagcactgc cgggtgctcg atgggtgaag
      121 tgattacctt aagtttgcgc cccaaacgcg ccgattgagc ggtggcaaaa ccctgcagaa
      181 acaggctgat actctcggtt gctcccgagg tgaaggtgat ttcgccaggg tcgcaggcaa
      241 tcaggtctgc gatcttttct cggctcgctt tcacgagcct cgcggcggcg ctgccgaagt
      301 catggtcgac gctcgaaggg ttgccataat ttcctttgag tatctggcaa tagtagtcga
      361 gcacgcctgg atcgaccggg gtggtggaga aatggtccaa atatatgcca tgcatgacag
      421 ctgcgctgtc agacatggca aactacacaa ccttcttctt ggtcttcacc atcaagaacg
      481 ccctcgagta cttcggcaag agtcttgtta ggtttgcgct gcttctgcag cttcacgcgc
      541 ttttcatgct ctgcaatgat ctggtctttg cgggcgatga gttcatcgag tgattcatcc
      601 tggctccagg tataccgacg accatcgggt aacacttttt cgaagctctt ggctttctcg
      661 aaaagcgcgg gatggttttc ggcgagcctc acccattcaa tcttacgctg aaaaaagcag
      721 aagtaacagc cggagcggga gcgccacttg tagtattcgg gcatgccgac ccccgattcg
      781 tcgagaatac gttcgacgtc ggctttcgtg atgccagcgt ctttgaacgg aaaaaccacc
      841 tggatggtat ctttcgtggc aacgtagccg ctgcggtctt cgtcggcacg aatggcgaca
      901 tagctcaccg cttcatcgtc accgataaag tcttcgagag gtttaatctt catctgaacg
      961 gtgcaccagc gagattgtgg tgatggcaga aagtcattgt atttctcaag aaaatgacga
     1021 aagccctgtg tcgcagcaag gcgctgcacc ttgataccca accgtgcttc aaggcgatcg
     1081 ataaagtcat aggtctcggc taactcttcg cccgtgtcgc aaaagaaata ctctatattg
     1141 gggactttgt cgcgcatgta gattgccagc gcagcagaat ctttgccacc cgaaataccc
     1201 agaatatgcc gcacggcttt ctgctcatct ttgggctttt tgaggactgc tgctgcgctc
     1261 tttttagcca cttgcgttac ccttacgctc gatcttaatg tgcggctttt tgtcctcacc
     1321 ggcaaaattt gtttctgtct ttggccctaa ggctatctct ttctgcaaca ggtctgcgag
     1381 cactgcctgc cgaatgcggt caggcgcttc tttccacacg ggcaaagcct caagctcggc
     1441 cttgaacttt tcagcgcgtt tttgctccgc cgcatcgacc caaataacct cacgggcttc
     1501 gtgaccatca gggttagtga tgctcaccag ccgcgcatca aacttaccgt tgccagccgc
     1561 ctgcactttc gccgccagct ggtgcaggtt caaaaacttc tgcgccagtg tagcaaggtt
     1621 ggcttcaaaa ctgtgtcggt cttcgtcgcg ccaggcttcg gcgggcttgt cggcgataac
     1681 cataatgaga ccttcaagcc attgttggtc agtcttatct ttgtcttgcg cggcattcat
     1741 gaaacggcgc agcaccgggt ctaagacctg ccccgcgagc aggcccgcca ttgccccgag
     1801 gtactggcgc agttcggtag ttttgggtat tctcaaacca tcgacgagag agcttcggca
     1861 atcttcgagc agagtaagat atgcattctg cagctcgtgc aaagatttgg tgagtgcctt
     1921 ggtcagtgtg cgcacagcct gagtgtcgtt ttgtttgtct gacagatcaa agccaagggc
     1981 tgcgggtatc tcttggaaaa tcaaggcgtc gggttcttt
//
LOCUS       DAOQJF010000480         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_45394_length_2015_cov_3.651020, whole genome shotgun sequence.
ACCESSION   DAOQJF010000480 DAOQJF010000000
VERSION     DAOQJF010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45394_length_2015_cov_3.651020"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PLF85_16825"
     CDS             complement(<1..1324)
                     /locus_tag="PLF85_16825"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE21384.1"
                     /translation="MLRIHLKPVLLLVALSIALPASCRRRSHQELGLALVNAVRDGSA
                     GEVRDLLASGASLSARDENGEILWRVALEHYNRKVWEVLTENGLDLSEPDSSGSLAIH
                     VAATNGLADASERIMKITAGRKQLNQAGDTALIAAARYGMAETLKVLKEFFQGANEQN
                     KAGDTALIESARSGNIKAVEEILKYRPSIPQANYGGQNAVSQAQYYYFIDIVRLLQGF
                     GAENPEASKALLAAVKIKDLGAAQAAVNRGANVNVVDDKGYSPLSYSVELADAPMIEY
                     LRSRGAMNALASRKLVLAIRADSIEVFNASLNDYADPNYTDKDLQTPLLIASAYGNYD
                     MVKTLLERGAQVPARDKDGYSAAFYAAQENRVRILDELIRRKADLVRKNNFGYTPLIL
                     AAAKGHVEAVRRLVEAGGGVNATADNGGTAAYWAAIKDHDEVVKILVGA"
     gene            1496..>2015
                     /locus_tag="PLF85_16830"
     CDS             1496..>2015
                     /locus_tag="PLF85_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia permease"
                     /protein_id="HNE21385.1"
                     /translation="MVRRKLTTSLLLIAGALAGSLYAADAKPAAPDFGKQIAELATSA
                     GTLKIAIDTLWTVVAGFLVFWMNAGFALVESGFCRSKNTVNILAKNFIVFALSTMSFW
                     IIGWGLMFGKGNDYMGLEGLFFLGGADNSPATGEAYKGVYDSMNWTGIPLNAKFFFQL
                     VFAGTAATIVSGA"
BASE COUNT          456 a          572 c          537 g          450 t
ORIGIN      
        1 ctgccccgac gagaatctta accacttcat catgatcttt gatcgccgcc cagtaggctg
       61 ctgttcctcc gttatcggca gtcgcgttga cacccccccc cgcctcaacc aggcggcgca
      121 ctgcctcgac gtgccctttc gcagccgcga gaatcagtgg agtataacca aaattattct
      181 tacgcacaag gtcagccttg cgcctgatca actcgtcgag gatacgcact cggttttcct
      241 gcgcggcata aaaagctgca gagtagccat ccttatcacg cgcgggaacc tgggcgccgc
      301 gttcgagcag ggtcttgacc atgtcgtagt taccgtaagc tgaagcgatc agcagaggcg
      361 tctgcaggtc tttgtctgta taattcgggt cggcatagtc attcagcgaa gcgttaaaca
      421 cctcgatact gtcggcgcga atggcgagta caagtttacg actcgccagg gcgttcattg
      481 ctccgcgcga gcgcaggtat tcaatcatcg gggcatctgc aagctccacc gaatacgaca
      541 gaggagaata acccttgtca tccaccacat tgacattggc accgcggttc actgcggcct
      601 gtgctgcccc gagatctttg attttgactg ctgccagcaa ggctttactc gcctccgggt
      661 tttcagcgcc aaagccctgc aacagccgaa cgatatcgat gaagtagtag tattgcgcct
      721 gcgagacagc attctgcccg ccgtaattgg cctgcggtat agatggcctg tacttgagga
      781 tttcttctac tgccttgata ttacccgaac gcgctgattc aatgagcgcg gtgtcaccgg
      841 ccttgttctg ttcattagct ccctgaaaga actccttcag cactttgagg gtttcggcca
      901 taccgtagcg tgcagcggca ataagcgccg tatcacctgc ctggttcagc tgtttcctgc
      961 cggcagtaat tttcataatc cgttcgctcg catcagccag cccgttcgtt gctgcaacgt
     1021 gaatggcaag gctgccagaa gaatccggtt cgctgagatc caggccgttt tcggtgagga
     1081 cttcccacac cttgcgattg tagtgctcga gggcgacacg ccagagtatt tcgccatttt
     1141 catcgcgcgc actgagcgag gcacccgagg ccaaaaggtc cctgacttcg ccggctgaac
     1201 cgtcgcgcac cgcattgacg agcgcaagcc cgagctcctg gtgcgaacgc ctgcggcacg
     1261 acgccggcag ggcaatgctc agcgccacca gcagcaggac aggtttcaaa tggattcgca
     1321 gcatgaaaca aaggctgcaa acgcagatac ctctgtcaat gaaataagga agactcacaa
     1381 aataggaaaa acgccggcct tacaattggc accaagtttg ctttagaagc gccagatgcc
     1441 gatgtccaat gcccatggca aaccgacgga cgcaaacaac aaggatggag gatttatggt
     1501 aagaagaaaa ctcaccactt cgcttctgct cattgcaggc gcgttggcgg gctcactcta
     1561 tgcggccgac gcaaaacctg ccgcgcccga tttcggaaag cagattgccg agctggcaac
     1621 cagcgccggg acactgaaaa ttgcgattga tacgctgtgg acagtggtcg ccggtttcct
     1681 ggtcttctgg atgaatgcag gctttgcact cgtcgaaagc ggattctgcc gcagcaagaa
     1741 cacggtaaac attctggcaa agaactttat cgtctttgcg ctctcaacaa tgtcgttctg
     1801 gattattggc tggggcctga tgttcggaaa aggcaacgac tatatgggcc tcgaagggct
     1861 attctttctc ggcggtgcag ataactcacc ggcgacaggt gaagcctata agggcgtata
     1921 cgacagcatg aactggaccg gaattccgct caatgcgaag ttcttcttcc agctggtgtt
     1981 tgccggcact gctgccacga tcgtttcggg agcag
//
LOCUS       DAOQJF010000481         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_45461_length_2013_cov_4.888662, whole genome shotgun sequence.
ACCESSION   DAOQJF010000481 DAOQJF010000000
VERSION     DAOQJF010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45461_length_2013_cov_4.888662"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="PLF85_16835"
     CDS             complement(<1..1776)
                     /locus_tag="PLF85_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21386.1"
                     /translation="MVNETPSTTKVFRSFEEVATSPEEAAQELALWNGKSEFPANIED
                     MIERGMNANLDRKSFLTFMGASLSMAALTACREPVEKIVPYVDRPMEYVPGQARYFAT
                     TQVSARGVTPVLVKTREGKPIKIEGLSSHPLFKGAAKADTFASIWDLYDPDRIKSPLK
                     KDGSSFKAAKWDEVIAEAAVALKGKARVLARTSFSPAETAARAKLGAAVVYDPTGAIS
                     EVVAGNRVSLGSAAIPAFRFDAADFILAIEADFLGTWLAPDLYTKQFSTRREPGGNMN
                     RLVVAESAMSVTGSNADKRLALVAGSHTTLALGLANLLLPGSGLAGDGNVRAAVQAYT
                     PAVVAKVTGLAEKQVIELAEELKKFKGTSLVVGGGVNARTAVAGELQIAVNLLNAILG
                     NEGKTVSTKGAMKANGETSEASEIEALIADMKSGKVETLVIDRANPVYELPVAAGFAE
                     ALKQVKNVIYIGDHINDTSSLATHILPPSHYLEAWSDALAHGHYGIVQPVIRPLFDTL
                     PVLEIFGRLKGDNTSAYEQVKKSHAANARGQGWDTTLSNGYAVIDAPGAGAARGFKSA
                     ALKAIKAPVEAQVGYRLSLIESVGLG"
     gene            complement(1806..>2013)
                     /locus_tag="PLF85_16840"
     CDS             complement(1806..>2013)
                     /locus_tag="PLF85_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE21387.1"
                     /translation="PHIKQLHKAGQPIKQACNACHGDVAGMKVVTQVESLNMGFCVNC
                     HRDHRNDAEYKNHGVNINCNTCHY"
BASE COUNT          408 a          539 c          561 g          505 t
ORIGIN      
        1 gccaaggccg acgctttcga tgaggctcag gcgatatccg acctgtgctt cgactggcgc
       61 ctttatggct ttcagtgcgg cacttttaaa gccacgcgct gcaccggcac cgggggcgtc
      121 gatgactgca taaccatttg agagcgttgt atcccaaccc tggccgcgcg catttgccgc
      181 atggcttttc ttgacctgtt cataagctga ggtattgtca cctttcaggc ggccgaagat
      241 ttccagtact ggcagggtat cgaacagtgg acgaatcact ggctgcacga taccgtaatg
      301 tccgtgcgcc agggcatctg accatgcctc gaggtagtgg ctcggtggca ggatatgcgt
      361 agccagcgag ctcgtatcgt tgatgtggtc gccaatgtaa atgacgttct ttacctgctt
      421 gagggcttca gcaaaacctg cagccacagg caattcgtag actgggtttg cacggtcgat
      481 aacgagtgtt tcaaccttgc cagacttcat gtcggcaatc agtgcctcga tttcagaagc
      541 ttcgctggtt tcgccgttgg ccttcattgc gcctttggtc gagacagtct tgccttcatt
      601 gccgaggatt gcattcagca ggttgactgc aatctgcagc tcaccagcca ctgctgtgcg
      661 ggcattcacg ccaccaccga caaccagtga tgtgcccttg aatttcttga gttcctctgc
      721 cagctctatg acctgttttt ctgcgaggcc tgttactttc gcaacaaccg caggagtgta
      781 ggcctgtact gcagcgcgca cgttgccgtc accggccagt cctgaacctg ggaggaggag
      841 gttagccaga cccagcgcga gagtcgtatg cgaaccggca acaagcgcca ggcgcttatc
      901 ggcgtttgaa ccggtgacag acatggctga ttcggccacg acgagacggt tcatgttacc
      961 gccgggttcg cggcgtgtag agaattgttt cgtatagagg tcaggcgcca gccatgtacc
     1021 gaggaaatcc gcttcgattg cgagaatgaa gtccgccgca tcaaaacgga atgcaggtat
     1081 agctgctgag ccaaggctca cacggttacc tgctacaacc tcgctgattg caccggtagg
     1141 gtcgtagacc actgcagcac caagtttagc gcgcgctgca gtttctgcag gtgagaagga
     1201 tgtgcgagcg agtacgcggg ctttgccttt cagcgccact gcagcttctg ctataacttc
     1261 atcccacttt gccgctttga atgatgaacc atctttcttc agcggcgact tgatccggtc
     1321 cggatcgtag agatcccaga ttgaggcaaa agtgtcagcc ttcgctgcac ctttgaacaa
     1381 tgggtgcgaa gagagtcctt cgatcttgat tggcttgcct tcgcgggttt ttaccaggac
     1441 aggggtaact ccccgtgctg aaacctgggt ggtcgcaaag tagcgagcct ggcctggaac
     1501 gtattccatc ggccggtcaa cgtaagggac aatcttttct accggttcgc ggcaggcagt
     1561 cagggctgcc atcgagagcg aagcgcccat gaatgtcagg aagctcttac ggtcgaggtt
     1621 ggcattcatg ccgcgctcga tcatgtcttc gatattcgca gggaattcgc ttttaccgtt
     1681 ccaaagagcc agctcctgcg ccgcttcttc gggcgaggtg gctacttctt caaatgaacg
     1741 gaaaaccttg gttgtgcttg gtgtttcgtt taccatgata tgatccttat tattccgaat
     1801 ccctattaat aatggcaggt gttacagttg atattcacac cgtggttttt gtactcagca
     1861 tcgttgcggt gatcgcggtg gcagttcacg cagaaaccca tgttgagcga ctcgacctgc
     1921 gtaacaactt tcatgccggc aacgtcgccg tggcatgcat tacatgcctg cttgatcggc
     1981 tggcctgcct tgtgcagttg cttaatgtgt gga
//
LOCUS       DAOQJF010000482         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_45951_length_2002_cov_4.525424, whole genome shotgun sequence.
ACCESSION   DAOQJF010000482 DAOQJF010000000
VERSION     DAOQJF010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45951_length_2002_cov_4.525424"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /gene="murD"
                     /locus_tag="PLF85_16845"
     CDS             <1..1295
                     /gene="murD"
                     /locus_tag="PLF85_16845"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNE21388.1"
                     /translation="YDKNPQAKPEGLHAGQLTYVESQTLEFARLRGSFDRLTLTPGVP
                     LRHPFILEARAAGIPVITEVAYCAPYLTGYKIIGITGTDGKSTTTAIIAQILRALGKP
                     AFECGNFGTPLSEIVLNPEHYANGTLSCELSSYQLEEPGKLNLDAAIFLNLAPDHLNR
                     YAGIEEYGLAKWNIAGLLRAGQPIVVAQNLLPENTTYWKNGHPLAAGKPRFISVDSQL
                     LRAANFAVQDGFLVSERASVRYDLRDCALAGAHNLSNLLFALEALHAVYPELLAQDLT
                     PVLRAIQPLPHRFEKIPQFAMPHVTFINDSKATTTQAAMTALKNAADPVFIFLGGQGK
                     GETYGELGKALKAKSARALIYGECREDMARDFRLAGFQDFTLHENLFKAFHTAKRMVG
                     QQKLASATLLLSPAVTSWDQFSSFEARGDYFRELVQSL"
     gene            complement(1290..1745)
                     /locus_tag="PLF85_16850"
     CDS             complement(1290..1745)
                     /locus_tag="PLF85_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21389.1"
                     /translation="MAATQSFVSPENWYSLQYPRTWEMEVVEGIPTFFDGISPYGGAL
                     QIFAINLAGAVNEEALEVSPFLRGKDLPDKMALFLSKQGVSNEGAQYFERDKTQMGAC
                     EYRSDGHFFLAMMAQRGTIFVLALYNCKGEPENEEAAAVGQMLQTLTIL"
     gene            complement(1754..>2002)
                     /locus_tag="PLF85_16855"
     CDS             complement(1754..>2002)
                     /locus_tag="PLF85_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNE21390.1"
                     /translation="EREALQAFVDQTNRAVNGVVKVKLYKGSAQVIARKSPNSLYSAA
                     LASFEKEDVYNQFDAEGFIRLFALTARNSYSEYKKLLG"
BASE COUNT          435 a          550 c          556 g          461 t
ORIGIN      
        1 ggtacgataa aaatccgcag gcaaaacccg aaggtttgca cgccgggcag ctgacgtacg
       61 ttgaatctca aacgcttgag tttgcccggt tgcgcggcag ctttgatcgg ctgacgctga
      121 cgccgggggt gccactgagg catccgttta tcttagaagc gcgcgccgcg ggtattccgg
      181 tgattactga ggttgcttat tgtgcgcctt acctgaccgg ttacaaaatt attggtatca
      241 ccggtaccga cggcaagagt acgaccaccg caatcattgc gcagatcctg cgtgcgctgg
      301 gaaagcctgc cttcgagtgc ggcaattttg gtacgccgct ttccgagata gtgctgaatc
      361 ccgaacatta cgcgaacggg accctgtcgt gtgaactttc gagctaccag cttgaagagc
      421 ctgggaagct taacctcgat gcggcgattt ttctcaacct ggcgcctgac cacctgaacc
      481 gttatgccgg tatcgaagag tacgggctgg caaaatggaa tattgcaggc ctgctgcgcg
      541 cgggccagcc tattgtggtg gcgcagaacc tgttgcctga aaatactact tactggaaga
      601 acggccatcc gcttgctgcc ggtaaaccgc gtttcatcag tgtcgacagc cagttgctgc
      661 gggcagcaaa ctttgctgtt caagatggtt tccttgtctc agaaagagct tctgtacgct
      721 acgacctgag ggattgcgcg cttgccggag cgcacaacct gagtaacctg ctttttgcgc
      781 tcgaagccct gcatgcagtg taccctgagc ttctggcaca ggatttgacg cccgtgctca
      841 gagcgatcca gcccctgccg caccgcttcg aaaaaatacc gcagttcgcg atgccgcacg
      901 ttaccttcat caatgacagc aaggcgacga ccacgcaggc tgcgatgacc gcgctgaaga
      961 atgccgcaga tccggttttt attttcctgg gcgggcaggg caaaggtgaa acttacggtg
     1021 aacttggcaa agcgctgaaa gcaaagtctg cgcgcgctct catctacggc gaatgccgcg
     1081 aagacatggc acgcgatttc cgcttagcgg gatttcagga cttcacgctg catgaaaacc
     1141 tgtttaaggc attccatact gccaaacgaa tggtggggca gcagaagctg gcttctgcta
     1201 cgctgttgct gtcgccggcc gtaacgtcgt gggaccagtt cagcagtttt gaagcgcgtg
     1261 gagactattt tcgtgagctg gtgcagtcgc tatagaattg tcagcgtctg cagcatttgc
     1321 ccaaccgctg cagcttcttc gttctcgggt tcgcccttac agttgtaaag ggccaggacg
     1381 aagatggtac cgcgctgagc catcatcgcg aggaaaaaat gtccatcgct gcggtattcg
     1441 caggcgccca tctgtgtctt gtcgcgctcg aagtactgcg caccttcgtt tgaaacaccc
     1501 tgcttactga gaaaaagcgc cattttgtcc ggcagatctt ttccgcgcag gaatggcgat
     1561 acctcaagcg cctcttcatt aacagcgcct gcgaggttga tcgcaaaaat ctgcagcgca
     1621 ccgccgtagg ggctgatacc gtcgaaaaat gtcggtatgc cctctacgac ttccatttcc
     1681 caggtgcgcg ggtactgcag cgagtaccag ttttccgggg aaacaaagct ctgcgtcgct
     1741 gccatgaagc tgcctagccc agaagcttct tatactcgct gtaagaatta cgcgcggtga
     1801 gcgcaaacag gcgtataaaa ccttctgcat caaactggtt gtagacatct tctttttcga
     1861 aagaagccag tgcagcagag tagagcgagt tgggcgactt gcgcgcaatt acctgagcgc
     1921 tgcccttgta cagcttcacc ttgacgacgc cgttgactgc gcggttggtc tggtcaacaa
     1981 atgcctgcag cgcttcgcgc tc
//
LOCUS       DAOQJF010000483         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_47278_length_1973_cov_4.014599, whole genome shotgun sequence.
ACCESSION   DAOQJF010000483 DAOQJF010000000
VERSION     DAOQJF010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47278_length_1973_cov_4.014599"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLF85_16860"
     CDS             complement(<1..210)
                     /locus_tag="PLF85_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNE21391.1"
                     /translation="MNESSAATVISAGLDAGADFVDLYEEETRNASVSLRDRKVENAT
                     AGIEYGIGLRLVYGTDVVYGYTGSGS"
     gene            320..682
                     /locus_tag="PLF85_16865"
     CDS             320..682
                     /locus_tag="PLF85_16865"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE21392.1"
                     /translation="MSTGQRQFKRLFAQRLGATTVSLNGSPQEPCTLMDISVGGLRLL
                     FERVLPEETFGAGAPVKGKIQSSEPGFVLNFEGKVVWNRRAALVGEAATMIGVTFLDY
                     TDLPETLLHLIDDFGQAD"
     gene            complement(712..1206)
                     /locus_tag="PLF85_16870"
     CDS             complement(712..1206)
                     /locus_tag="PLF85_16870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNE21393.1"
                     /translation="MLHPHIEVRGSDIEGRGLFATAPIKSGELVWRKDDNEKYYSQAE
                     IDQFTPEQKKNFYNYCYQVGPDKFYGTPEGKAGDDADYMNHSCDPNTWFEADGSMTAR
                     RDISAGEEITYDYATSESRPDFQLACRCGSPLCRHTVKGDDLKRSAELRLRYGHHIMP
                     HVLA"
     gene            complement(1187..>1973)
                     /locus_tag="PLF85_16875"
     CDS             complement(1187..>1973)
                     /locus_tag="PLF85_16875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNE21394.1"
                     /translation="IVLLIGIEKAAAHVFHVEGTMISTPAAPPTEAQQKNTPGDSPLE
                     VRPTRFGKGVFARREFAAGTRILQFRGRVYTRAEYLSKVNPVKCHYMQIGDNAFLGPT
                     TTPDNFVNHCCEPNAGLVIENGKAWLVAIQIIRPEDEITFDYSTSMAEDHWEMDCACG
                     AASCRGRVRDFKHLQSPLQQKYIELGIVPDFVIRSAGLENPNDLFHTADTRPAKGASR
                     RRVPAGQNGSNNIGTSLNHAPRNDGVHAGAELGSSLNHASPAY"
BASE COUNT          415 a          532 c          555 g          471 t
ORIGIN      
        1 cgacccgctg cctgtgtagc cgtagactac gtctgtacca tagacgaggc gcaagcctat
       61 gccatattcg atgcccgccg tggcgttttc aaccttgcgg tcgcgcaggc tgacagacgc
      121 atttctggtt tcttcttcgt aaagatcgac gaaatcggcg cccgcatcga gtcctgcgct
      181 gattacggtc gcggcagagc tttcattcat atggcattac tcctgcgccg ttcacgacgc
      241 caatgaaaac cgctaacaag tgcatagacg ggggtaatat acgctttacg cgaacgagcg
      301 tccgtaagtt tttcccggca tgtctaccgg ccagaggcaa ttcaagcggc ttttcgctca
      361 acgattgggg gcgacgactg taagcctaaa cggttcaccg caggagccct gcaccctcat
      421 ggatatttcc gttggtgggc tgcggctgct ttttgagcgc gtgctgccag aggagacgtt
      481 cggggcaggg gcgcctgtca agggaaagat ccagagcagc gaaccaggct ttgtacttaa
      541 ttttgaagga aaggttgtct ggaaccgaag agccgcgctg gtcggcgaag cggccacaat
      601 gattggggtg acttttttgg attacaccga cctgccagag acactgctgc acctgattga
      661 cgatttcggg caggccgact agcagtctga gaggtcgctc ccgacggccg tctatgccag
      721 cacgtgcggc ataatgtgat ggccataacg caggcgtagt tcggcagatc gcttcaggtc
      781 atcacctttg actgtatggc gacaaagcgg tgacccacac ctacaggcta gctgaaagtc
      841 aggtctcgac tcgcttgtgg cataatcata cgtgatttct tcgccggcag atatgtcacg
      901 ccgggccgtc atactgccat cggcttcaaa ccatgtattg ggatcgcagc tgtggttcat
      961 gtaatcggcg tcgtcgccgg ccttgccttc cggcgtgccg tagaacttat caggacctac
     1021 ctgatagcag tagttgtaga aattcttttt ctgttcaggt gtgaactggt caatttcggc
     1081 ctgcgagtaa tatttctcgt tgtcgtcttt acgccagacc agctcgccgc ttttgatcgg
     1141 ggcagttgca aacaggccgc gaccttcaat gtcgctgccg cgcacctcaa tatgcgggtg
     1201 aagcatggtt caaggaggat ccgagctccg cgccggcatg gacgccgtca ttgcgcggtg
     1261 catggttcaa ggacgtacca atgttattgc tgccattttg tcctgcgggg acgcggcggc
     1321 gcgaggcacc ttttgcaggc ctggtgtccg ccgtatggaa caagtcattg ggattttcca
     1381 atccggcaga acgaatgacg aaatccggca caatacccag ctcgatatac ttttgctgaa
     1441 gaggagattg caggtgttta aagtcgcgta ccctgccgcg gcaactggcc gcgccgcagg
     1501 cacagtccat ctcccagtgg tcttcagcca tcgaggtgga gtaatcgaag gttatttcgt
     1561 cttccggtct gatgatctga atcgctacca gccaggcttt gccgttttca atcacaaggc
     1621 cagcattcgg ttcgcagcag tgattcacga aattatcggg cgtagtcgtg ggaccaagaa
     1681 aggcattatc cccgatttgc atgtagtggc atttaacggg atttaccttg ctcaggtatt
     1741 ctgcacgcgt gtagacgcgt ccgcgaaact gcaaaatacg cgtaccggca gcaaattccc
     1801 gccgggcaaa aactcccttg ccgaaccggg tcggtcgaac ctccagaggc gaatcgcctg
     1861 gagtgttttt ctgctgggcc tccgttggtg gcgcagcggg tgtggatatc atggttccct
     1921 caacatgaaa gacgtgtgct gccgccttct ctataccaat caatagtaca atc
//
LOCUS       DAOQJF010000484         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_47526_length_1968_cov_5.418191, whole genome shotgun sequence.
ACCESSION   DAOQJF010000484 DAOQJF010000000
VERSION     DAOQJF010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47526_length_1968_cov_5.418191"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PLF85_16880"
     CDS             <1..1476
                     /locus_tag="PLF85_16880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE21395.1"
                     /translation="HGRQAYRLMEASRAPRFAAENDADRVCYAPADIFAAGERNHHIS
                     NTAYPIAHEQTFKPYRVKRAEQEPVILGGFTPGTELCLYSHIPFCEKRCYFCEYTVVG
                     RDELTQTAEYMQALNHEVELYAEKLGHPALSGWDIGGGTPSFVSAELMADHIAAVRMM
                     FSFAPGFAISIETTPRIASLEPDKIVAYREMGIERISMGVQVTEPDLLKALGRDTNGL
                     EHNYRATENIRRAGFRKFNLDLMYGFAGQSDESWRTTLEHSIAMAPDYITLYRMRYKL
                     TRISDQAGRVTMEQVLRQARMAKELLAAAGYVANPGKNTYSRLAGDTGTSAYLTRRVI
                     KGTPYLGVGLGAQSFTSSTISYNSGSVGKNLAPYLKDVASNRLPLQDLYHLPRAHMMA
                     KMIAVSFYFGEINLAAFYARFGITLEAAYPQATLFALQEGLMHYTDSVNGAELNDLDH
                     RSLSLTEKGARHFNGTIALFFAPSVQHYLLGRNQAEIYAAV"
     gene            1463..>1968
                     /locus_tag="PLF85_16885"
     CDS             1463..>1968
                     /locus_tag="PLF85_16885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE21396.1"
                     /translation="MPQFDFANVLFAGPCNRTCPFCIGKQVPESVNTNNLDTFPPKNW
                     QAFCGKIKELGITELIFTGTTTDPQLYQHEQALIGKARADLPGIKISVHTNGVLATKK
                     IEIFNAYDRATISLPSLSEKTYRRMMGHGQVPDLARLVAEAKIPIKISTIVSEHNIDE
                     IPNLLAQFA"
BASE COUNT          482 a          585 c          505 g          396 t
ORIGIN      
        1 catgggcggc aagcgtatcg gctgatggaa gcttcgcgcg caccacgttt cgctgctgaa
       61 aacgacgccg accgggtatg ttatgcgccg gcagatattt ttgccgccgg cgagcgcaac
      121 caccatatct caaacacagc ttaccctata gcgcacgagc agacttttaa accttaccgg
      181 gtaaaacgtg ctgagcagga acctgtcatc ctggggggat ttacccccgg tacggagctc
      241 tgcctctatt cgcatattcc cttctgcgaa aaacgctgct acttctgtga atacaccgta
      301 gtcggccgcg acgaactcac gcaaaccgct gagtacatgc aggcactcaa ccacgaggtg
      361 gagctctacg ctgaaaagct gggtcaccct gccctgtcgg gctgggatat cggcggagga
      421 actccctcgt ttgtttcggc ggaactgatg gccgaccaca tcgcagccgt gcgcatgatg
      481 ttttcgtttg cgccgggttt tgccattagt attgaaacga ctccccgcat tgcgtcacta
      541 gaacccgata agattgtggc ctaccgcgaa atgggtatcg agcgcatcag catgggagtg
      601 caagtcaccg aacccgacct gctcaaggcg ctcgggcgcg acacgaatgg actcgagcac
      661 aattaccgcg ccaccgagaa catccgccgc gcgggtttcc gaaaattcaa cctcgacctg
      721 atgtacggct tcgcggggca gtcggacgaa tcgtggcgca caacgctcga gcactcaatt
      781 gcgatggccc ccgactacat cacgctctac cgcatgcgct acaaactgac acgcatctca
      841 gaccaggccg gacgagtgac tatggaacag gtactgcgcc aggcacgcat ggcgaaagaa
      901 ctactcgctg cggcgggcta cgtcgcaaac ccgggcaaga acacctacag ccgcctcgca
      961 ggtgacacgg gcacgagtgc ctacctgacc cgccgcgtga tcaaaggaac tccatacctg
     1021 ggagtcggtc tcggtgcgca gtcattcact tcttcaacga tctcgtacaa ttcaggatca
     1081 gtcggcaaga acctggcgcc gtacctgaaa gatgtggcgt cgaatcgcct gcccctgcaa
     1141 gacctctacc acttgccgcg cgcgcacatg atggccaaga tgatcgccgt cagtttctac
     1201 ttcggtgaaa ttaatctcgc ggctttctat gcgcgcttcg gcatcacgct cgaagctgct
     1261 tatccccagg cgacgctatt tgctctacag gaagggctca tgcattatac cgattcagta
     1321 aacggagctg aactgaatga tcttgatcac aggtcgctgt cactgaccga aaagggagca
     1381 cggcacttca acggtacaat agccctgttc tttgcgccgt cggttcaaca ctatctgctg
     1441 gggcgcaacc aggctgaaat ctatgccgca gtttgatttt gccaacgtcc tttttgccgg
     1501 accgtgcaac cgcacttgcc cgttttgtat cggaaaacaa gttcccgaat cggtaaacac
     1561 caacaatctc gatacctttc caccgaagaa ctggcaggca ttctgcggaa agatcaaaga
     1621 actgggaatc accgaactca tcttcaccgg cacaaccacc gacccacagt tgtaccagca
     1681 tgagcaggca ttgatcggca aagcaagagc agatttaccc ggcatcaaaa tttctgtcca
     1741 cacgaacgga gtgcttgcaa caaagaaaat tgaaatcttc aatgcatatg accgcgccac
     1801 gatctcattg ccgagcctga gcgaaaagac ctaccgcagg atgatggggc acggccaggt
     1861 gcctgatttg gcacgcctgg tggcagaggc gaagataccc atcaagatca gcacgattgt
     1921 ttccgaacac aacatagacg aaataccgaa cctgctggcg caatttgc
//
LOCUS       DAOQJF010000485         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_47620_length_1966_cov_4.390372, whole genome shotgun sequence.
ACCESSION   DAOQJF010000485 DAOQJF010000000
VERSION     DAOQJF010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47620_length_1966_cov_4.390372"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1550)
                     /locus_tag="PLF85_16890"
     CDS             complement(24..1550)
                     /locus_tag="PLF85_16890"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE21397.1"
                     /translation="MLAAAVAALANMLLGSLDAADKGETGPEPKTIRRQAALEEINTL
                     AQEFAPTFSPDGKTMVFSSTRSSGKGGFSHLYVSHLKDGKWGAPEPLHSVNSQFNDET
                     PYLSNDGKILLFASDRDGSLELPRDAKGQVRVSFDIYLAQWDGKTWSNPRPVQEINTP
                     WNEKSPSISRDGRYLFFSSWPFGDMKRSRIRMLDLQAKGNVIDDLPPHINSGNQETAL
                     VPGEDNTRYYFSSRRPGGKGGWDIWQTRFSDGKWAEPEPVPGNVNTEGNEAFLAVSQG
                     NYFISSTFNAEKPDYDLNIEPIKPEAAKQWNVQLIDASTRQKLTGTVKLEVLEADSDK
                     KSSHSTQGSGNGIVFKAPVSVGGEEVVRLNGSAEGYLPGSEDTDTATLTEGTYLLPLR
                     KLEKNASFDIRSIYFDFDSARIKKSSEPSLRMVLEFLKQNPKAEFEIIGHTDLAGDAE
                     YNQSLSEKRAASVKTWLVENGIDKGRLTTSGAGKSRPVVAKRGKPFDEQNRRTEFKLK
                     "
     gene            1701..1943
                     /locus_tag="PLF85_16895"
     CDS             1701..1943
                     /locus_tag="PLF85_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21398.1"
                     /translation="MKTAISIPDSLFKKAEEFAKSNRISRSELYQRALAAFLSDAAYA
                     DVTRMLDEVYGDEILRVPRVIRRLQSQALKNGDEAW"
BASE COUNT          391 a          500 c          555 g          520 t
ORIGIN      
        1 gagagaggtg tatagcccct ctctcatttt aatttgaatt ctgtacgccg gttctgttca
       61 tcgaatggct tgccgcgttt ggcgactacc gggcgcgact tgccggcgcc cgatgtggtg
      121 agccggccct tgtctatacc attttcgacc agccaggttt ttacactcgc cgcgcgtttt
      181 tcagagagcg actggttgta ttctgcatca cccgcaaggt cggtgtggcc gatgatttca
      241 aattcagctt tggggttttg cttgagaaat tcaagtacca tgcgcagtga aggttctgaa
      301 gatttcttga tgcgcgccga gtcgaaatcg aaatagatcg aacggatatc aaaactcgcg
      361 ttcttttcca gcttgcgcag gggcagcagg tacgtgccct cggtgagcgt tgctgtatcg
      421 gtatcttcgc tgccgggaag ataaccttcg gcgctgccgt tgagcctgac aacttcttcg
      481 ccgccgactg ataccggcgc cttaaagaca ataccgttgc cgctgccctg agtgctgtgg
      541 ctcgatttct tgtcactgtc cgcttcaagt acctcgagtt ttactgtgcc cgtgagtttt
      601 tgccgcgttg aagcatcgat cagctgcacg ttccattgtt ttgctgcttc gggtttgatg
      661 ggttcgatgt tcagatcgta gtccggcttt tctgcgttaa acgtgctcga aataaaatag
      721 tttccctgcg aaactgccag gaatgcttca ttcccctcgg tgttcacatt acccggaaca
      781 ggttcgggct ctgcccattt gccgtcgctg aaccgtgttt gccagatatc ccagccgcct
      841 ttgccacctg gcctgcgcga tgaaaaatag tagcgcgtat tgtcttcgcc tggaaccagc
      901 gctgtttcct ggttgcccga gttgatgtgc ggcggcaggt catcgatcac gttacctttc
      961 gcttgcaggt cgagcatacg aatgcgcgag cgtttcatat cgccgaaggg ccagctgctg
     1021 aaaaagaggt agcggccatc gcggctgatc gacggcgatt tttcattcca cggtgtgtta
     1081 atttcctgca cggggcgggg gtttgaccag gttttgccat cccactgcgc aaggtagata
     1141 tcaaagctta cacgtacctg accttttgca tcgcgcggca actcgagact gccgtcgcgg
     1201 tcagaggcaa atagcaaaat cttgccgtca ttgctgaggt agggggtttc gtcgttgaat
     1261 tgtgaattga ccgagtgcag cggctctggc gctccccatt ttccgtcctt taggtgagag
     1321 acatagagat gcgaaaaacc acctttgccg gacgagcgcg tcgacgaaaa aaccatcgtt
     1381 ttaccgtcgg ggctgaaggt gggggcaaac tcctgcgcca gcgtgttgat ctcttcgagc
     1441 gctgcctgcc ggcggattgt cttcggttct ggccctgttt cgcctttgtc ggctgcgtcg
     1501 agagaaccga gcagcatatt ggccagcgcc gcaacggcgg cggcgagcat gattcgcctc
     1561 atggttgatc ttcggttgtt tgctgaatcg ctgaagcaga aaaggaggag ttgcatcgct
     1621 tgggtaagaa gatctccgca atgtgcaggt ttagcggttt gtttattgac agaaggtata
     1681 acttttgtta tactgcgggt atgaagacgg ctatcagtat cccggattcg ctcttcaaaa
     1741 aagccgaaga attcgccaaa tcaaaccgga tatcgcgcag tgaattgtat cagcgcgcgc
     1801 ttgcggcatt tctcagcgat gcggcctatg cagatgtcac ccgcatgctt gatgaggtgt
     1861 acggtgacga aatactccgt gttcctcggg taattcgccg cttgcagtca caggcgctaa
     1921 aaaatggcga tgaagcgtgg tgaaatctgg tgggccgaac tggacg
//
LOCUS       DAOQJF010000486         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_47671_length_1965_cov_4.265969, whole genome shotgun sequence.
ACCESSION   DAOQJF010000486 DAOQJF010000000
VERSION     DAOQJF010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47671_length_1965_cov_4.265969"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PLF85_16900"
     CDS             <1..1040
                     /locus_tag="PLF85_16900"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21399.1"
                     /translation="LDRWRWWELVPAAVLIAYDILVLLMPPVFFTEFLNVALVALLAL
                     GLQILVYTTMAVRRGQRGANILLGGIMLLFAASINDVFFNFAFSATIELVPLALLVFT
                     LMNVVGLELMEYRLRKFSAQLDISISERRTSLERLAPGLAQRFLQASSSKSAMLSSRE
                     KVPVRFAALLADLRGFTAISESQSPEEVFGLINRYLEHVVPPIMANGGQVLEYQGDGI
                     LAYFTLGADSCLRAAVAMQQELRRGYETGALPELSMGIAMHSGSGVLALLGNYQRLEP
                     AVVSPAILEVQDLEMLCSQHKVPYVFSYRFFNELSRDAQSQAKILTDLAGSPVFTLAA
                     PAGSQEPAGHT"
     gene            1155..1511
                     /locus_tag="PLF85_16905"
     CDS             1155..1511
                     /locus_tag="PLF85_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE21400.1"
                     /translation="MQTVLAVDDSETMRQMVKMTLQTGSYNVILASDGEEALNTFLST
                     TGVDLVVTDINMPKLDGLGLIKAIRERNQDVPILALTTESEDTMRKKGAEAGANGWMT
                     KPFKPAQFIEVVRRII"
     gene            1557..>1965
                     /locus_tag="PLF85_16910"
     CDS             1557..>1965
                     /locus_tag="PLF85_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21401.1"
                     /translation="MRSFPLLIAALLFVMQCRGREYLTGLPAYRSSPAKSVIILRAET
                     EGRREARLVIVEPDTGTATEVSSPQVPVAQSVKHDEIIAERDLLLTRSGSIWSLKGSD
                     AAGHTYTGHQLSPDGRHLLARGGNAVSVTPLFGE"
BASE COUNT          429 a          523 c          533 g          480 t
ORIGIN      
        1 cacttgatcg ctggcgctgg tgggagctgg taccggccgc agttcttatt gcctacgaca
       61 tacttgtcct attgatgccg ccggtatttt ttactgaatt tctgaacgtc gcgctcgttg
      121 cattactcgc actcggcctg cagatactcg tctatacgac tatggccgta cggcgaggtc
      181 agcgcggagc aaatatcctt cttggtggca taatgctgtt gttcgctgct tcaattaacg
      241 atgttttctt taactttgcc ttttctgcca cgattgaact ggtacctctt gctttgctgg
      301 tcttcaccct gatgaatgtt gtcgggcttg agctcatgga atacagatta cgcaagtttt
      361 ctgctcagct ggatatcagc attagtgaac gccggacaag cttagaacgg ctggcgccag
      421 ggttggcgca acgattcttg caggcgtcgt cgagtaagtc agccatgttg tcgtcccggg
      481 aaaaggttcc ggtgcgtttt gccgcgctgc ttgccgatct gcgcggcttt acggctatat
      541 cagaatccca aagtccagaa gaggtctttg gcctcattaa tcgttacctg gaacatgttg
      601 tgcctcccat tatggcgaac ggtggccagg tgttagaata ccagggtgac ggtattctgg
      661 cctattttac tcttggggcc gattcctgcc tcagggccgc agttgcgatg cagcaagaac
      721 tccgccgtgg ctatgaaaca ggagcgctac cagaattgag catgggcatc gccatgcatt
      781 ccggatcggg ggttctggcg ctgctgggca attaccagag gcttgagcct gcggtcgttt
      841 caccagcaat actcgaggtg caggatctcg agatgctctg ctcgcaacat aaggtaccgt
      901 acgtgttttc ttaccgtttt tttaatgaac tttcccgtga cgcacagtcg caggcaaaga
      961 ttctgacaga cctggccggt tctccggttt ttacgctcgc tgctccggcc gggtcacagg
     1021 aacctgccgg ccatacgtaa atctacggat cgtaacccta ccgagcccac accgtaggct
     1081 cgataattga cttcagaccc aaaatgtgct aaaaacagcc attccaattg aggggaaacc
     1141 cgggggaggc ttagatgcaa acagtactag cggtagatga ttcggaaacc atgcgccaga
     1201 tggtcaaaat gacgctccaa acaggaagct acaatgtcat tctggcaagc gacggtgaag
     1261 aggcgctgaa tactttcctg agcacgacag gtgtcgacct ggtggtgaca gacatcaaca
     1321 tgcccaagct cgatgggctg ggactgatca aagcgattcg cgaacgcaac caggatgtac
     1381 ctatcctcgc gctcacgaca gaatcagaag ataccatgcg caaaaaaggg gctgaagccg
     1441 gcgccaacgg ctggatgact aaacctttca agccggcaca gtttatcgaa gtcgtcagac
     1501 gcattatctg atagccaatt cgcttgccgc cgacgtgggc aaatcacttt ggcgctatgc
     1561 ggagtttccc tcttctgatt gcggcattgc tttttgtgat gcagtgccgc ggtcgggaat
     1621 atctcaccgg cctgccggca taccgcagtt ctccggcaaa atctgttatc atcctgcgcg
     1681 ccgaaaccga aggtcgcaga gaggctcgcc ttgtcattgt tgaacccgat accggtactg
     1741 ccaccgaggt cagctcacca caagttccgg tcgcccagag tgtaaaacac gatgagataa
     1801 ttgccgagcg cgatctgctg ctcacgcgca gcggaagcat ttggtccctg aaaggttctg
     1861 acgcagccgg ccatacgtat acggggcatc agctctctcc cgatggccgg catttgcttg
     1921 cgcgtggtgg aaatgccgta tctgtgacgc cgctttttgg cgagg
//
LOCUS       DAOQJF010000487         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_48252_length_1953_cov_4.760801, whole genome shotgun sequence.
ACCESSION   DAOQJF010000487 DAOQJF010000000
VERSION     DAOQJF010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48252_length_1953_cov_4.760801"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1644
                     /locus_tag="PLF85_16915"
     CDS             229..1644
                     /locus_tag="PLF85_16915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE21402.1"
                     /translation="MRTQDLSRAQSRDTVRVQFLGAAGFVTGSRTLIEYGGFRAYVDC
                     GLYQGPKYVEERNYLPLESDPSTIDAIILTHAHIDHSGLIPRLYREGFRGAIYCTRPT
                     ARLLEIILPDAGNIQEEEFSRLSKNELRKLGLEAPLFTREDGKLVLELVKTVQYNRTF
                     RVGPFSFCFTWAGHILGAAHLNVWRKGFSMVFSGDIGPESTFFHKPLKRPRMAENVVI
                     ESTYGDRVRENEDYEKKFRDAVRYIVERRGVLLLPAFAVGRAQTTLYVLFRLMQEKKI
                     PQIPIALDSPMAVKATQTYSRFKNELTAEVVRSGFFEFLRSRQVKLIESAEESKALAE
                     SPGPMIIVSASGMCTGGRVVHHLEQRLGDTRNAVLFTGYAGEGTLAHEIMSGVGRVNI
                     YGREIPVRALVTQVQSFSAHADQTGLIAWMRQFDKKSIKRIYINHGEDAARQHLAGEL
                     AFMESQIELPRYQSTYYLPCG"
     gene            complement(1685..>1953)
                     /locus_tag="PLF85_16920"
     CDS             complement(1685..>1953)
                     /locus_tag="PLF85_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNE21403.1"
                     /translation="ELLEAKGKRPFFELALPHTSTMLRQAFGRLIRTERDKGVVALLD
                     SRVWQKTYGKTLIANLPRVQVLRTFAELERANAQYGLLNYEPHA"
BASE COUNT          415 a          539 c          562 g          437 t
ORIGIN      
        1 ctctggaact ggtgtcgagc agggctgctt cgatcgtgga gatgcgcttc ttcgccgggc
       61 cccagctcaa cctggtaccg ctcaagaaca ccaacctgct gagcatctac gcgcagggta
      121 acgtatcgac tggcggaaac tacggcgtcc acgcgggagc acgcatcgcc tggtaattct
      181 gagctcagca tttttgcggg gggttaaata gcaccccgcg atttccaggt gcgcacgcaa
      241 gacttgtccc gagcgcagtc gagggatacg gtcagggtgc agtttctcgg cgcggccggt
      301 tttgtaaccg gctcgcgcac tcttattgaa tacggcggat ttcgtgccta cgtcgactgc
      361 ggtctctacc agggaccaaa atacgtcgaa gagcgcaatt acctgcccct cgagtctgat
      421 ccttcaacca ttgatgcaat cattcttacg catgcacaca ttgaccactc agggttgatt
      481 ccgcggctct accgcgaagg atttcgtggt gccatctact gcacacggcc tacggcacgt
      541 ttactcgaaa ttattctgcc cgacgcgggt aatatacagg aagaagaatt cagccggctc
      601 tctaagaacg agttgcgcaa gctgggcctc gaagctccgc tttttacccg cgaagacggc
      661 aagctggttc ttgaactcgt aaagactgtg cagtataacc gcacgtttcg cgtcggtccc
      721 tttagctttt gtttcacctg ggccggccat atcttaggcg ctgcgcacct caatgtctgg
      781 cgaaagggtt tcagcatggt gttcagcggc gacattggtc ctgaaagtac gttcttccat
      841 aaaccgctca agcgcccgcg catggcagaa aatgtcgtca ttgaatcgac gtacggcgac
      901 cgcgtgcgcg aaaacgaaga ttacgaaaaa aagttccgcg acgcagtgcg ctatatcgta
      961 gaacggcggg gagttttact gttaccggcc tttgccgtgg gcagggcgca gacgacgctc
     1021 tacgtgctgt ttcgactgat gcaggaaaag aagatcccgc agatacccat tgcgctcgat
     1081 tcgccgatgg cggtcaaggc gacgcagacc tacagccgct tcaagaatga gctcaccgcc
     1141 gaagtggtgc gctcaggttt tttcgaattc ctgcgctcgc gccaggtcaa gctgatcgaa
     1201 tcggccgaag agagcaaggc gctcgccgaa tcacccgggc ccatgatcat tgtcagcgct
     1261 tcgggtatgt gtacgggggg cagggtggtg caccacctcg agcagcgcct tggcgatacg
     1321 cgcaacgcag tactctttac cggttatgcc ggcgagggca cactcgctca tgaaataatg
     1381 agtggggtgg gcagggttaa tatttatgga cgcgaaattc ctgtccgcgc gctggtgacg
     1441 caggtgcagt cgttctcggc ccatgccgac cagacagggc tgatcgcctg gatgcggcag
     1501 tttgataaga agagcatcaa gcgcatctac atcaatcatg gcgaagatgc ggcgcgccag
     1561 cacctggccg gtgagctcgc tttcatggaa tcgcagatcg aactgcctcg ttaccagagc
     1621 acgtactatc tgccgtgtgg ttagagtttt tgtccgcatg catagtgagg gtagaccacc
     1681 gtcactatgc atgcggttcg tagttcaata acccatattg tgcattcgcc cgttcaagtt
     1741 cggcaaaggt gcgcagaacc tgcacgcgcg gcagattggc gatgagggtt ttgccgtaag
     1801 tcttttgcca gacgcggctg tcgagcaatg cgacgactcc tttatcgcgt tctgtgcgaa
     1861 tcagtcgccc gaatgcctgc cgcagcatgg ttgaagtgtg tggcagcgcc agctcgaaga
     1921 atggccgttt accttttgct tccagcagct cag
//
LOCUS       DAOQJF010000488         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_48295_length_1952_cov_3.909331, whole genome shotgun sequence.
ACCESSION   DAOQJF010000488 DAOQJF010000000
VERSION     DAOQJF010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48295_length_1952_cov_3.909331"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..923
                     /gene="truA"
                     /locus_tag="PLF85_16925"
     CDS             111..923
                     /gene="truA"
                     /locus_tag="PLF85_16925"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNE21404.1"
                     /translation="MKTAITIEYDGAPYFGFQLQKQQNSVQAELEKALGIVLRQRVRV
                     YCAGRTDTGVNALGQVVHFAAEYRQSIPELVYSLNSLLPKDIAIRHAAHVPEDFHARF
                     SCLGREYVYVICNAPYRPGNLGFKSLWVRGDLDWAPVRAAIPHLVGEKDFATFTRAAI
                     VKSGERTVRRIDGVDVIESQPYIFLYIRGSGFLHNMIRILTGTLLDVARGKISPQQME
                     EIVANADRLGAGVTQKPDALYFLHAEYKDYPQQGAEHWLRRLLVRETTGLAG"
     gene            complement(912..>1952)
                     /gene="tgt"
                     /locus_tag="PLF85_16930"
     CDS             complement(912..>1952)
                     /gene="tgt"
                     /locus_tag="PLF85_16930"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNE21405.1"
                     /translation="TPVFMPVGTQATVKAISQEDLEEIGFRLILGNTYHLYLRPGSDL
                     ICDAGGIHNFMSWPYAVLTDSGGYQAFSLSDNVKLTDHGVIFRSHLDGSEHLFSPERT
                     VQIQREIGSDIMMPIDDCAPYPSDDKRLKESLVRTHRWLAECKAHWQKDPKHQTLFGI
                     SQGGVNPVLRAESARAVADIDLPGYAIGGLSVGEPLEYYVPSLAAAIEHLPKEKPRYV
                     MGVGTIPEILNGVELGVDMYDCVLPTRNARNGQVFTSTGKVNLRNLKHARSDDAIDSA
                     CSCKVCKKYSLGYIRHLHKQNEILGLMLSTYHNLYFMHGFMKNMRNAIVAGKFAEYRA
                     HWLGVFGETSAR"
BASE COUNT          429 a          512 c          564 g          447 t
ORIGIN      
        1 tttgtgcgtc tggaatttca tatgcagaac ctgcataatg ataaagcatt gatacgacat
       61 aaagcaaaat atgtttgttg attggcggga ttctcccgcc aatcaacgag atgaagacgg
      121 cgattaccat cgagtatgat ggggccccct attttgggtt ccagctgcag aagcagcaga
      181 attccgtcca ggcagagctg gaaaaagcgc tgggtatcgt gctcaggcaa cgggtgcggg
      241 tatactgcgc cggcagaacc gataccggcg tgaacgcgct ggggcaagtc gtgcattttg
      301 cggctgaata ccgccaaagt atacccgagc tggtctattc gctgaacagc ctgctaccca
      361 aagatattgc cataaggcat gctgcgcacg tccccgaaga ttttcacgcg cggttttcgt
      421 gtctcggccg cgaatacgtc tatgtcatct gcaacgcgcc ctaccgtccg ggcaatctcg
      481 gcttcaagtc actgtgggtg cgcggcgacc tcgactgggc gccggtgcgc gctgctatac
      541 ctcaccttgt cggtgaaaaa gacttcgcaa cttttacccg cgcggcaatc gtcaaaagtg
      601 gtgaacgtac ggtaaggcgg attgatggcg ttgacgtcat tgaatcgcag ccgtatatct
      661 ttctctatat acggggttcg ggctttttgc acaatatgat ccgcattctg accggaactc
      721 tgctcgacgt cgcgcgcgga aaaatttcac cgcagcagat ggaagagatt gtagccaacg
      781 ccgaccgcct gggtgcgggt gtgacccaaa aacctgatgc gctctatttc ttacacgcag
      841 agtacaaaga ttacccacag cagggtgccg agcactggtt gcggcggttg ttggtgcgcg
      901 agactacagg cctagcgggc tgatgtctcg ccgaacaccc ctaaccagtg agcgcggtac
      961 tcggcaaatt tacctgccac gatcgcattg cgcatgttct tcataaatcc gtgcataaag
     1021 tagaggttgt ggtacgtgct gagcatgagc cccaaaattt cgttctgctt gtgcaggtgg
     1081 cgaatatagc cgagcgaata ttttttacag accttgcagc tgcaagcact gtcgatcgca
     1141 tcatcagaac gagcatgctt gaggttgcgc aggtttactt tgccggttga agtgaaaacc
     1201 tgaccattgc gcgcgttgcg cgtcggcaaa acacagtcat acatatcgac acccagttcg
     1261 acgccgttca aaatttcggg tatcgtaccc acacccatca cgtagcgcgg cttttctttg
     1321 ggcaggtgtt caatcgccgc ggcaagcgac ggtacatagt attcgagcgg ttcgccgacg
     1381 gagagaccgc cgatggcgta gcccggcaga tcaatgtcgg cgaccgcgcg tgcgctctca
     1441 gctcgtagta cagggttcac tcccccttgt gaaatgccga agagagtctg atgttttgga
     1501 tctttctgcc aatgtgcctt gcattcggca agccaacggt gtgtgcgtac gaggctctct
     1561 ttgaggcgct tgtcgtcaga ggggtagggt gcgcagtcgt cgatcggcat catgatgtca
     1621 gaaccaatct cgcgctgtat ctgcaccgtg cgctcgggcg aaaacagatg ctcgctgccg
     1681 tcgaggtgcg aacggaagat gacgccgtgg tcggtgagtt ttacattgtc gctcagggaa
     1741 aatgcctgat agccgcctga atccgtgagc actgcgtacg gccacgacat gaagttgtgg
     1801 atgccgcccg catcgcaaat gaggtcagaa cccgggcgca ggtacaggtg gtaggtgttg
     1861 cccagaatta ggcgaaagcc gatctcttcg aggtcttcct ggctgatcgc cttgaccgta
     1921 gcctgtgtgc ccacgggcat gaagacgggg gt
//
LOCUS       DAOQJF010000489         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_48571_length_1946_cov_6.334744, whole genome shotgun sequence.
ACCESSION   DAOQJF010000489 DAOQJF010000000
VERSION     DAOQJF010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48571_length_1946_cov_6.334744"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLF85_16935"
     CDS             <1..945
                     /locus_tag="PLF85_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNE21406.1"
                     /translation="TALVPEASLRHYYRLRFDDGRTLIGVNEDATSATTNMPNVIAVQ
                     KFLREHGVAVPEIYFADPAAGVMLQQDLGDLSLNQGLKDNPDKMDLLYQDAIRHMFSW
                     QRLADDGQCPAFRLSFDVEKLMFEFEFFITHTLLGYYGAKPSEGDLSRLRAQFLEIAE
                     SLAAPERKVFTHRDYHSRNILMVGASTPLSHLAAKQYIIDFQDARMGLMQYDLCSLLC
                     DAYAPLSPERRAALLDFAFEQGKGIHRQTRGEFDHYWQLSAFQRTVKAMGTFGRQAAL
                     GRDDFAAYLNPAWQTLKEVSEQAPNLRQWAEKLEQYAG"
     gene            complement(1007..1264)
                     /locus_tag="PLF85_16940"
     CDS             complement(1007..1264)
                     /locus_tag="PLF85_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21407.1"
                     /translation="MRIFSRTREWSGGGVQLGKTKRLAIVASTDEHFVTARISRLAKI
                     PFMPVLHLDVIAQRQNAADQSAACPGSLSSGFPQFTGYGIP"
     gene            1488..1844
                     /locus_tag="PLF85_16945"
     CDS             1488..1844
                     /locus_tag="PLF85_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNE21408.1"
                     /translation="MAKHGIREADLEESFIRSGGKGGQNVNKVSTAVRLSHPPSGIEI
                     KCMKHRTQGMNRYAARVMLCEKLEQKERDASAIENPVHKKKRLAKLDRARKAKRKAKA
                     KAARKPATPNWKNEDW"
BASE COUNT          467 a          531 c          543 g          405 t
ORIGIN      
        1 acagcactgg tgcctgaggc atcgctgcgg cactattatc gtttgcgttt tgacgatggc
       61 cgcacgctga tcggcgttaa cgaagacgcc acctctgcca ccactaacat gccgaatgtc
      121 atcgcggtgc agaagttttt gcgtgagcac ggcgttgcag tacccgagat ctattttgca
      181 gacccggccg caggggtgat gctgcaacaa gatctcggcg acctgagcct taatcagggg
      241 ctcaaagaca accccgacaa gatggattta ctctatcagg acgccatccg ccacatgttc
      301 agctggcaac gccttgctga cgacggccaa tgcccggctt tcaggctcag cttcgatgtc
      361 gaaaagctga tgtttgaatt cgagtttttt atcacgcaca ccttgctggg gtattatggc
      421 gcaaaaccca gcgaggggga tttatcacga ttgcgcgcac aatttctgga aattgcagaa
      481 tcactcgcag ctcccgaacg caaggttttc acccatcgtg attaccattc gcgtaacatt
      541 ctgatggtcg gggcttcgac tccgctcagc cacctcgctg cgaaacaata catcatcgat
      601 tttcaggatg cgcgcatggg cctcatgcaa tacgacctct gctcgctgct ctgcgatgcg
      661 tatgcgccgt tgtcgcctga acgccgcgcg gccctgcttg actttgcttt tgagcagggc
      721 aaaggaattc accgccagac acgcggcgag tttgaccatt actggcagct gagtgcattc
      781 cagcgaacgg ttaaggccat gggtacattc ggcaggcagg ctgcactggg gcgcgatgat
      841 tttgccgcat acctcaatcc ggcatggcag acactgaaag aggtgagtga acaggccccg
      901 aacctgcggc aatgggctga gaaacttgaa caatatgcgg gataacttca gagctgagga
      961 tttccatagc tgcaaaagaa attccagtat tatatctcat gaatagctat ggaattccat
     1021 agcctgtgaa ttgcggaaac cccgaactca gcgagcctgg acaggcagcg ctttgatcgg
     1081 cggcgttttg ccgctgcgcg attacatcga ggtgcagtac cggcataaag ggaattttgg
     1141 cgaggcgcga aatgcgcgcg gtgacaaaat gttcatctgt gctggcgacg atggcgaggc
     1201 gtttggtttt gcctaattgc actccgccgc ccgaccattc cctggtgcgc gagaatatcc
     1261 gcacggctcc agcgttcgtg ccgccgcagc cgaaccacct caaaagcaat gcgcatcaaa
     1321 ctaaaaaagt gcgccatgga ttccggaacc tgccccaaaa gctccttttc aggagccttt
     1381 ggggataatc cacgtgacaa gcagagtctg tctttctgtt tttgattata gtggcttcgc
     1441 tctctttacc agtctcaccc gccaagatcg ccgcgctcga agcgcgtatg gcgaaacatg
     1501 gcatccgcga agccgacctc gaagagtcgt tcatccgcag tggcggcaag ggcgggcaaa
     1561 acgtgaacaa ggtttcaacg gcggtgcggc tgtcgcaccc acccagcggt atcgaaatca
     1621 agtgcatgaa acaccgcacg cagggtatga accgctacgc cgcgcgcgtt atgctgtgcg
     1681 agaagctcga acagaaagag cgcgacgcca gcgcgattga aaatcctgta cataagaaga
     1741 aacgcctggc aaaactcgac cgcgcgcgca aggcgaagcg caaggccaag gcgaaagcgg
     1801 ccagaaagcc tgccacacca aactggaaga acgaagactg gtaattacgc gactggagaa
     1861 ggatctgatt tccggggttc gccgtagacg gcgatatgct gcggtgagat acggctgaaa
     1921 gtcaggctga aggtgtcttt gaggtc
//
LOCUS       DAOQJF010000490         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_50404_length_1909_cov_3.972492, whole genome shotgun sequence.
ACCESSION   DAOQJF010000490 DAOQJF010000000
VERSION     DAOQJF010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50404_length_1909_cov_3.972492"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1467)
                     /gene="argH"
                     /locus_tag="PLF85_16950"
     CDS             complement(49..1467)
                     /gene="argH"
                     /locus_tag="PLF85_16950"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNE21409.1"
                     /translation="MASKQEKLWHGRFEAPPSKITEEISESISYDNALYRVDIRASKA
                     HARMLSKVGVLTADEFAAIEKGLSQVETEIGQGKLAFSAALEDIHMHVESRLTEIIGE
                     TGKKLHTGRSRNDQVAIDTHLFVREALTEHRAQLKALLQRLLQLAQENLGKIWIGYTH
                     LQIAQPVLLSHYLLAYFWQFARDLELLDFVGQSMRFSPLGSAALGGANYPVDRAQTAE
                     ELGFAAPYPNSMDAVANRDYQLNYHFWVTRLYLHISRFCEDIIIYNTTEFGYVTLGDA
                     VTTGSSIMPQKKNPDIAELLRGKAGRVNGNLQALITNVKGLPLTYNRDLQEDKVYLFD
                     SIGQARQGLLGLTEILHHIRFNEERALENLSRGFSLATDIADYLVREKGIPFRDAHAV
                     AGNSVAYAEKNGKRLETLAAADWQQIAGYDPKFPADFFTPQASVKRRQGYGSTNPEAV
                     AKQLAEARALLAAMGNPPASHT"
     gene            complement(1516..>1909)
                     /locus_tag="PLF85_16955"
     CDS             complement(1516..>1909)
                     /locus_tag="PLF85_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21410.1"
                     /translation="YDILGTYFLKVQADPKLFAPLKNSGLKIRFNYTGPDAAIALDGS
                     TEDKSKQIMTGDAARKFEPEVEFTLQADVAHYFWHGQVNLMQALARKEISPKGNLPRA
                     MRLLPLLEPLYELYPNYLRSRDLAKLAL"
BASE COUNT          397 a          541 c          550 g          421 t
ORIGIN      
        1 aagggtacac tctcggggta tgaagttgtc ctgcgcgtgc accagctgct aggtgtgtga
       61 agctggggga ttgcccatag cagcaagcaa agcccgcgct tcagccagct gcttcgcaac
      121 cgcctcggga tttgtcgaac cgtaaccctg cctgcgcttc accgatgcct gtggcgtaaa
      181 aaagtccgcg gggaatttcg gatcgtaacc ggcaatctgc tgccagtcag cggcagcaag
      241 cgtttcgagg cgtttgccgt tcttctcggc gtaggccacc gaattgccag caaccgcatg
      301 ggcgtcacgg aacggaatac ccttttcacg cacgaggtaa tcggctatat cggtagccag
      361 ggaaaagccg cgcgaaagat tctcgagcgc tcgctcttcg ttaaagcgga tatggtgcag
      421 aatttccgta agacccagga gtccctgccg ggcctgcccg atactgtcga acaggtagac
      481 cttgtcttcc tgcaggtcac ggttgtacgt gagcggtaac cctttcacgt tggtgatcag
      541 tgcctgcagg ttaccgttca ccctgcccgc tttaccacgc agcagctcgg cgatatcagg
      601 gttctttttc tgcggcatga tcgatgaacc cgttgtcaca gcgtcaccca gggtcacgta
      661 accaaactcg gtggtattat agataatgat atcttcgcaa aagcgcgaga tatgcagata
      721 aagccgcgtc acccagaaat ggtagttcag ctggtagtca cggttggcga ctgcgtccat
      781 gctgttcggg tacggtgccg cgaaacccag ctcttctgct gtctgtgctc ggtcgaccgg
      841 gtaatttgcc ccgccgagcg cggctgagcc gagcggagaa aaacgcatgc tctgcccgac
      901 aaagtcaagc aactcgaggt cgcgcgcgaa ctgccagaaa tacgcgagca ggtagtgaga
      961 aagcagcacc ggctgcgcaa tctgcaggtg cgtgtagcca atccagattt tgccgaggtt
     1021 ttcctgagcc agctgcagca ggcgctgcag cagtgccttc aactgagcac ggtgctcggt
     1081 gagcgcctcg cgcacaaaca ggtgcgtgtc gatcgccacc tggtcgttac gcgagcgccc
     1141 ggtgtggagc ttcttgccgg tttcgccgat gatctcggtc aggcgcgact cgacgtgcat
     1201 atgaatgtct tcaagcgccg cggaaaaagc gagctttccc tgcccgattt ccgtttcaac
     1261 ctgcgaaaga cctttctcta tcgccgcgaa ttcgtcagcc gtgagaactc ccactttgct
     1321 aagcatgcgt gcgtgtgcct tcgaggccct tatgtcaacg cggtagagcg cgttgtcata
     1381 cgagatcgac tctgaaattt cttcagttat ctttgacggg ggcgcctcaa agcgcccgtg
     1441 ccacaatttc tcctgcttcg aggccatgcg cgtgcaacgt agagacgcac ttcagtgcgt
     1501 ctctacaaaa acaaatcaga gcgccagctt cgcgaggtcg cggctgcgca ggtagttcgg
     1561 gtacagttcg tagagcggct cgagcagtgg cagaagcctc atcgcacgcg gtagattgcc
     1621 cttggggctg atctctttgc gcgccagcgc ctgcatcagg ttcacctggc catgccagaa
     1681 atagtgcgcg acatcggcct gcagcgtgaa ctcgacctcg ggttcaaact tgcgcgcggc
     1741 gtcaccagtc atgatctgct tcgacttgtc ttctgttgaa ccgtcgaggg caatagctgc
     1801 atccgggccc gtgtaattga aacgaatctt gaggcccgag ttcttgagcg gagcgaaaag
     1861 ttttggatcg gcctgtacct taaggaaata cgtaccgagg atatcatag
//
LOCUS       DAOQJF010000491         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_50406_length_1909_cov_3.667745, whole genome shotgun sequence.
ACCESSION   DAOQJF010000491 DAOQJF010000000
VERSION     DAOQJF010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50406_length_1909_cov_3.667745"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PLF85_16960"
     CDS             complement(<1..666)
                     /locus_tag="PLF85_16960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE21411.1"
                     /translation="MAIIIDSHAHFEPRMLGEDDWLRKLDVAGVDKVALIPSMNDPLP
                     PVPELLLGGVRLLMQSGFTRPIAELLHRATLTDAGNLRLLTGEYKIYAQPDNESAFAL
                     VRRHPDRFMSWAFLNPRADTKVLETLERWRHEEGMIGVKLHPHWHDYTTDLLFPLFAR
                     LEELGLPALIHLGFGRRGDYRAIAEKFPRLKIIAAHAGFPFYQDMWNFTRQHNIYVDL
                     SSPY"
     gene            complement(690..1499)
                     /locus_tag="PLF85_16965"
     CDS             complement(690..1499)
                     /locus_tag="PLF85_16965"
                     /inference="COORDINATES: protein motif:HMM:NF016733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HNE21412.1"
                     /translation="MKAYKYLLLLFVAGHMGCVQLREEDDMDELAAAELNKLALYQSE
                     TSVVPGELPALPSPYLEPDENLLLNGSFEMPVLDSPWAVFATGQVPGWQAVWVDTSCT
                     STPQIELQSKTLWGIDAEFNQYAELDTNSACGSDARISLSQKFVTQPNHIYRLTFRVR
                     ARDAEHTIGLKLSIGESYAQQITPPADAWQALTVNFEATAAETTLVFTETGDGDTYGS
                     FVDAVEVREITVDTERMPKPEKGKKKMRGKKRHSFDGGRRHKDCQSMRHKP"
     gene            1616..1858
                     /locus_tag="PLF85_16970"
     CDS             1616..1858
                     /locus_tag="PLF85_16970"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNE21413.1"
                     /translation="MPATLSLKEVPAETHRTLKERAARNHRSLNSEILAILEAAVKSQ
                     KIETDEYVRRARALRSRFTGKIDDAELQAAKRTGRA"
BASE COUNT          459 a          529 c          516 g          405 t
ORIGIN      
        1 gtaggggctt gagagatcga cgtagatatt gtgctgccgc gtgaaattcc acatgtcctg
       61 gtaaaaggga aagccggcat gcgccgcgat aattttgaga cgcgggaatt tctccgcgat
      121 agcacggtaa tcgccacgcc tgccaaaacc gagatggatg agcgcaggta gcccaagctc
      181 ttccagtcgt gcaaacagcg gaaagagcaa atcggtcgtg tagtcgtgcc agtgcgggtg
      241 cagcttcaca ccaatcattc cctcttcatg ccgccaacgc tcaagggttt caagcacttt
      301 ggtatcggcg cgcgggttta aaaaagccca ggacatgaac cggtcaggat gccgccgcac
      361 gagcgcgaaa gctgactcgt tgtctggctg cgcatagatt ttgtactcgc ccgtcaaaag
      421 acgcaggttt cctgcatccg taagggtcgc ccggtgcaag agctcggcga tcgggcgcgt
      481 gaagcccgac tgcatcagta gccgcacacc gcccagcaaa agttccggta cgggcggaag
      541 cggatcgttc atcgagggaa tcagcgcaac tttatcgacc ccagccacgt cgagcttgcg
      601 cagccagtca tcttcgccga gcatacgtgg ctcgaaatga gcgtgtgaat ctataatgat
      661 tgccataaac caagggtcgc ttaggcaacc tatggtttat gccgcataga ctggcaatct
      721 ttatgccgac gaccaccatc gaaagagtga cgcttcttac cgcgcatttt ctttttccct
      781 ttctctggtt tgggcatacg ttccgtatcg acggtaatct cccgcacttc gacagcatcg
      841 acgaaagagc cataggtatc accgtcccct gtttcggtaa atacaagggt tgtttctgca
      901 gcagttgcct cgaaattcac ggtcagcgcc tgccaggcgt ccgcaggcgg agttatctgc
      961 tgcgcgtaac tttcacctat gctgagtttt aacccgatcg tgtgctcagc gtcgcgcgcc
     1021 ctcacacgaa atgtcagccg gtagatatga ttcggctgcg ttacgaattt ctggctaagg
     1081 ctgatacgcg cgtcgctgcc gcaggccgag ttcgtgtcga gctcagcgta ctggttgaat
     1141 tctgcgtcga tgccccagag cgtctttgac tgcagctcaa tctgcggtgt actggtacaa
     1201 cttgtatcaa cccagacagc ctgccagcca ggcacctggc ctgtcgcgaa gactgcccag
     1261 ggcgaatcga gcaccggcat ttcaaaggag ccattgagca ggaggttttc gtcgggttca
     1321 aggtaaggcg aaggcaatgc gggcaattcg cccggcacta ctgatgtctc agactggtag
     1381 agagcaagct tgttgagttc ggctgctgcc agctcgtcca tatcgtcttc ttctcttaac
     1441 tgaacgcagc ccatatgtcc tgccacgaac agcagcaaca ggtatttata cgctttcata
     1501 ccatgaagag ctggtatgcc ctgaaacagg acatactttt ccggttgagc gaaaaagaac
     1561 aggcaacaaa tgaaaacaat ttgacaaact gccatttcag aaccattttg gtaccatgcc
     1621 agcaacactc agcctgaaag aagtaccggc agaaacgcac cgcacgctca aagaacgcgc
     1681 cgcacgcaac caccgcagtc tgaacagcga aatactcgcg atactagaag ctgcggtaaa
     1741 gtcgcagaaa atcgagaccg acgagtatgt gcgtagagca cgcgcgctac gcagcagatt
     1801 cacgggaaaa atcgacgacg ctgagctgca ggcagctaag cgaacagggc gcgcatgatc
     1861 gcggccttcc tggaggcgtt ctggtcatca agcgcctgga ttccggcct
//
LOCUS       DAOQJF010000492         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_50409_length_1909_cov_3.425027, whole genome shotgun sequence.
ACCESSION   DAOQJF010000492 DAOQJF010000000
VERSION     DAOQJF010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50409_length_1909_cov_3.425027"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLF85_16975"
     CDS             complement(<1..253)
                     /locus_tag="PLF85_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21414.1"
                     /translation="MDVRFLAKGCLCLVFPLFSLCARPYSGRVQVAPETLLQAADRGL
                     DYTGAADRAIDGPEGDLLAFIRLGNRLDSAGRYFHGLHVF"
     gene            354..1559
                     /locus_tag="PLF85_16980"
     CDS             354..1559
                     /locus_tag="PLF85_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE21415.1"
                     /translation="MFQIANFQQNAFVIVEGKAAPGFYIVRQGKVRLTAEMPIPGEDP
                     NLILNPGDFFGVVSAMSGHPHIETAQCLAATSLINVGRDQFGVLIQKNAPIAMKIIRY
                     FSQRLRIIDRAITKLTFHSTVEEDPQLMFNIAQFYYGKQEMKHAAYAYQRYLQYYPNG
                     SFAAQSKMALQNMSAPMSVPTPAGNALARSFADGQMVFIEHEPGNELYIIQGGKVKIT
                     KVVNDNEVLLAVLNPGDIFGEMALLENKPRSASAVAFGNVTTLAINKQNFEAMVQAQP
                     QLATKLITLLSERIWLAYRQLANLLITDPVGRMYDMLLTQVQKKKVAIQPKTSYVFDI
                     GSAELLKMLGYAADKGEEYLITMLSDKNMRLDQGKFICKDLAELEKQVQFIRKRAAAE
                     RQREQAKLG"
     gene            complement(1563..>1909)
                     /locus_tag="PLF85_16985"
     CDS             complement(1563..>1909)
                     /locus_tag="PLF85_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21416.1"
                     /translation="RRGLGFGCGEEPLASYLGQVDRNSKKYVEKLEMDKQKRYCMAYL
                     LAGEMFGVFKGCLELIEMVEKFTADSRPERLNYDRKGAFESAMQTLQYLKASLNSEIE
                     KFLGVLVAADVI"
BASE COUNT          465 a          573 c          510 g          361 t
ORIGIN      
        1 caaagacatg caaaccatga aaatagcggc ccgcagagtc aagccggttg cccagacgga
       61 taaaagccag aagatcgcct tcggggccgt caatcgcgcg atcggcagcc ccggtgtagt
      121 ccaaaccgcg gtcggcagcc tgcagcagcg tctcaggcgc aacctggacc cggcctgagt
      181 acggccgcgc gcacaggcta aaaaggggga agacgaggca aaggcacccc ttagccagaa
      241 acctgacgtc catggcgcac aagccacgac ccagcccgtc ccgtaaacca ctcagtagct
      301 cttcaccttg ccaaggcatc tgccgataat taaaagggat aaatagcccg cctatgttcc
      361 agattgcgaa ttttcagcag aatgcttttg tcatcgtcga gggcaaagcc gcacccggat
      421 tttatatcgt tcggcagggc aaggtacgtc ttacggccga gatgccgatt cccggtgaag
      481 acccgaacct gatactcaac cccggcgact tttttggcgt cgtttcggcg atgagcgggc
      541 acccgcacat tgagacggcg cagtgcctgg cggcaacctc gctaatcaac gtcggccgcg
      601 accagttcgg ggtacttatc cagaagaatg ccccgattgc gatgaagatc atccggtatt
      661 tctcgcagcg gctgcgcatc atcgaccgcg ccatcacgaa gctgacattc cacagcacgg
      721 ttgaagaaga cccgcagctg atgttcaata tcgcgcagtt ctactacggt aaacaggaaa
      781 tgaagcacgc cgcatacgcg taccagcgct acctgcaata ctaccccaac ggttcatttg
      841 ccgcgcagtc caaaatggcg ctgcagaaca tgagcgcgcc aatgtcggta ccgaccccgg
      901 ctggcaacgc gctcgcgcgc tcttttgccg acggacagat ggtgttcata gaacacgaac
      961 ccggcaacga actctacatt atccagggtg gcaaagtcaa gatcaccaaa gtcgtcaacg
     1021 acaacgaagt actgctcgcg gtactgaacc cgggcgacat ctttggcgaa atggcgctgc
     1081 tcgaaaacaa accgcgctcg gcgtcggcgg tggcgttcgg caatgttacc actctggcga
     1141 tcaacaagca gaactttgaa gccatggtgc aggcgcagcc acagctcgcg accaagctga
     1201 tcacactgct cagcgagcgt atctggcttg cataccgcca gctggcaaac ctgctgatta
     1261 ccgaccctgt gggccgtatg tacgacatgc tgctgacaca ggtgcagaag aagaaagtgg
     1321 cgattcagcc gaagacgagc tacgtattcg atatcggctc tgccgaactt ctgaagatgc
     1381 tcggttacgc ggccgacaag ggcgaagagt atctcatcac catgctgagc gacaagaata
     1441 tgcgtctcga ccagggcaag tttatctgca aagaccttgc cgaactcgag aagcaggtgc
     1501 agtttatccg caagcgcgca gcagcagaac gccagcgcga acaggcaaaa ctcggctaat
     1561 cctcagatta catcagcggc tacgagcacg cccagaaact tctcaatctc actgttgagg
     1621 cttgctttca ggtactgcag ggtctgcatc gcagattcaa aggcaccttt gcggtcataa
     1681 ttgaggcgtt ctggcctcga atcggcagtg aacttttcca ccatttcgat cagctcgagg
     1741 cagcccttga atacgccaaa catctcgccg gcaagcaggt aggccatgca gtagcgcttc
     1801 tgcttgtcca tttccagttt ttccacgtac ttcttgctgt tgcggtcgac ctgtcccagg
     1861 tagctggcga gcggttcctc gccgcaccca aagcccaggc cgcggcgcc
//
LOCUS       DAOQJF010000493         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_51369_length_1890_cov_5.227248, whole genome shotgun sequence.
ACCESSION   DAOQJF010000493 DAOQJF010000000
VERSION     DAOQJF010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51369_length_1890_cov_5.227248"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLF85_16990"
     CDS             complement(<1..760)
                     /locus_tag="PLF85_16990"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNE21417.1"
                     /translation="MPDFAAINGNDSAQFNELQRNASTALSDQKKRPVDRRGVPLPIR
                     KSGYDALDKNSFLKLLVTQLAKQDPTNPMNDREFISQMAQFSSLEQMNNVANSMNKLR
                     ASQANQLIGKMINGKDFVTEKPVQGIATKATYDRNGEVFLSVNGRMIKLDDVTEVTEV
                     TPIAPPQPVGNVSRETLTSGNAPLESALKQRPEDGSRGAVARETAPAANVRSLSAVEA
                     PAPKVNPAQATQAYEANQKAVNVSRETPDNAKINK"
     gene            complement(753..>1890)
                     /locus_tag="PLF85_16995"
     CDS             complement(753..>1890)
                     /locus_tag="PLF85_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21418.1"
                     /translation="IQKEQAEPKSRAVTVTRSDRELLEALGLINRKTEIADKKPAVGT
                     LRAAMDATALRQPQKTEKKPVVFKGESPTHTLTVLEKTPDLVLMSSDGLKKLGEKLGL
                     SLLGKVHEKLSEKNRPNELAQQTARAEIRSESVQPMVQQKSQKAGAGDHESSADHQQS
                     RGSRQAAVKNVSRETSATEAPVSLRQAQADNVAMVNPSNHDTTALRTQSNAIPTSEIR
                     LSEAANSARAAENIRTAVENPMVRPDLVRQFNEIISRAQVLVTDTQNAQFSVKLFPRE
                     IGRMEIDLKLVDGEIRGKIVVESEDVKNEMQNFLQNREQGGNAEQFDMNKIDIEVRSG
                     NQNAQNPQQAPDTQELLQNLVTQSASAAYSAIESTTGQGNALYA"
BASE COUNT          301 a          514 c          551 g          524 t
ORIGIN      
        1 ctttatttat ttttgcattg tctggtgttt cacgtgaaac attcactgcc ttctggttcg
       61 cctcgtaggc ctgcgtggcc tgggctgggt ttactttagg tgcgggcgct tcgactgcgc
      121 tcagcgaccg cacgttcgca gcgggcgccg tctcgcgagc aactgcaccc cgcgacccgt
      181 cttcgggtct ctgcttcaga gcggattcga ggggcgcgtt gcctgaagtt agtgtttcac
      241 gtgaaacatt gcccacaggc tggggtggcg caatcggagt aacttctgtc acctcggtaa
      301 cgtcgtcgag cttgatcatg cgcccgttca cgcttaagaa aacctcgccg ttgcggtcat
      361 aggtggcctt cgtcgcgata ccctgcacgg gtttttcggt cacaaagtct ttaccgttga
      421 tcattttgcc gatcagctgg ttcgcctgag acgcgcgcag cttattcatc gagttggcga
      481 cgttgttcat ctgctccagc gaagaaaatt gcgccatctg tgaaatgaac tcgcggtcgt
      541 tcatcgggtt ggtggggtcc tgcttcgcga gctgagtgac caatagtttc agaaagctgt
      601 tcttatcgag cgcatcgtag ccgctcttgc gaatgggcag tggtacacca cgccggtcca
      661 ctggtctctt cttttggtcg ctgagcgcag tcgaagcgtt gcgctgcagt tcattgaact
      721 gtgcgctgtc gttgccgttg atggcggcga aatcaggcat agagcgcgtt accctggccc
      781 gtcgtactct ctatcgcgct gtaggctgca gatgccgact gcgtcacgag gttttgcagc
      841 agttcctggg tatcaggcgc ctgctgcgga ttttgtgcgt tctggttacc gctgcgcacc
      901 tcgatatcga tcttgttcat atcaaactgc tctgcgttgc cgccttgctc gcggttctgc
      961 aggaaattct gcatctcgtt cttcacatct tcgctttcaa cgacgatctt gccgcgaatt
     1021 tcaccatcga cgagtttcag atcgatttcc atgcggccga tttcgcgggg aaagagttta
     1081 acgctgaact gcgcgttctg cgtgtcggtc acgagtacct gtgcacggct aatgatctca
     1141 ttgaactggc gcacgaggtc agggcgcacc atggggtttt cgaccgcggt gcggatattt
     1201 tctgccgcac gtgcagagtt tgcggcttca gacaggcgga tttcagaggt cggtatggca
     1261 ttcgactgcg tgcggagtgc tgtcgtgtca tggtttgacg ggttcaccat cgcaacattg
     1321 tcagcctgag cctggcgaag gctgacaggg gcttcggtag cggatgtttc acgtgaaaca
     1381 ttcttcaccg ctgcctggcg gctgccacgc gactgctggt gatctgcaga agactcatgg
     1441 tcgcctgcac cggctttctg gcttttctgt tgaaccatgg gttgtacaga ttcgctccga
     1501 atttcagcac gcgcggtttg ctgtgccagc tcattgggcc ggttcttttc gctgagtttt
     1561 tcatgtacct tgcccaggag cgagaggccg agcttctccc cgagtttctt cagaccgtcg
     1621 ctgctcatca gcacgaggtc aggggtcttt tcgagtactg tcagtgtatg cgtaggcgat
     1681 tcacccttga agacaaccgg ctttttctcg gtcttttgtg gctggcgcag cgcggttgca
     1741 tccattgcgg cgcgcagtgt gccgaccgct ggcttcttgt cggcaatttc cgtcttgcgg
     1801 ttaatcagtc cgagcgcttc gagcagttca cggtcgctgc gcgttacggt aacggcgcgg
     1861 cttttgggtt cagcctgctc tttctgtatc
//
LOCUS       DAOQJF010000494         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_53979_length_1843_cov_4.899329, whole genome shotgun sequence.
ACCESSION   DAOQJF010000494 DAOQJF010000000
VERSION     DAOQJF010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53979_length_1843_cov_4.899329"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLF85_17000"
     CDS             complement(<1..248)
                     /locus_tag="PLF85_17000"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNE21419.1"
                     /translation="MGYKFRRQHVVRKYILDFFCPAANLGIELDGKVHLDQAEYDEFR
                     SVGLLQTGIRILRFKNEAILANIGEVLAIISATLQNIST"
     gene            complement(322..>1843)
                     /locus_tag="PLF85_17005"
     CDS             complement(322..>1843)
                     /locus_tag="PLF85_17005"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE21420.1"
                     /translation="KIAFTIAARNLLRHKGKSLVIGAILFLGALIMTLGNAVIGGMNR
                     GLRENIVERFTGHVVVISDTQENPNILFTPMGKDIALIKHFDRVKSVLTSQQPIAGYV
                     PAGRGFSLVLTEEGDIDFAMLLGVDFPAYNAMFKENVKLVEGKLLGPQERGMLVSQGR
                     RTRFFNDQNIWLKPAGFPLEEKNFSDEAKKYRDKMIVKDSLVLMGASGDSTAFDVRTH
                     IKGIVRYEFLDDYWKFFNIMDIETFRECFQWVTSTDAAQNIKADTKALMAQSDNLDSL
                     FNESTVVSSSVGKAEIAKSLKKLKQKTAEQKVVTDAGAYNVVFVKLKDGYDIDKGVRE
                     LNAAFKAAEAPAQAVTWQKAIGQLGDMAGIIRGALIGFVFFIFFVAIIIIMNTLSMAA
                     LERTAELGMMRAVGAQKRFITIMFLIETACLAILFGGAGILLGSLSVPVLKAMRITAE
                     NNILQLLFGGKTFAPYLSAGDIVLGVAELAWVTIMAAIYPIIVARRITPLDAISRD"
BASE COUNT          417 a          513 c          488 g          425 t
ORIGIN      
        1 gtgctgatat tctggagcgt cgctgaaatg atcgccaata cttccccaat attggccaat
       61 atcgcctcat tcttgaacct caagatacga atacccgtct gtaacaatcc aacagagcga
      121 aactcatcat attccgcctg atccagatgc accttgccgt ctagctcaat tcccaggttt
      181 gcggctgggc agaaaaaatc gagaatatac ttacgcacaa cgtgctggcg acggaatttg
      241 taacccataa gttttcctat tttcaaatgc gaccataaga ttgcttctgc ctgtgggggg
      301 tggggacaat ttcttttcat attaatccct cgaaatggcg tcgagcggcg taatgcgccg
      361 ggcgacaatg atcgggtaga tcgcggccat gatagttacc caggcaagct ccgccacgcc
      421 gaggacgatg tcacccgcgc tgaggtatgg cgcaaacgtc ttaccgccaa agagcaactg
      481 caaaatgttg ttctcggccg tgatgcgcat cgccttgagc accggcaccg acagcgaacc
      541 cagcaggatg ccagcaccac caaacaggat tgcgaggcat gctgtttcga tgagaaacat
      601 gatggtgata aagcgcttct gtgcgccgac cgcgcgcatc ataccgagct cggctgtgcg
      661 ttcaagcgcc gccatcgaga gcgtgttcat aataatgatg atcgcaacga agaatataaa
      721 gaagacaaaa ccgatgagcg cgccgcgaat gatgcctgcc atgtcgccca gctggccaat
      781 cgccttttgc caggttactg cttgcgccgg ggcttctgcc gccttgaacg cagcgttgag
      841 ttcacgcaca cccttgtcga tgtcgtagcc atctttcagc ttcacgaaca cgacgttgta
      901 agcaccggca tcggtcacga ctttctgttc ggcagtcttc tgcttcagct ttttgaggct
      961 tttcgcaatt tcggccttgc cgacggagga tgacaccacc gtgctctcgt tgaagagcga
     1021 gtcgaggtta tcgctctgcg ccatgagcgc cttggtatcg gccttgatgt tctgcgcggc
     1081 gtcggtcgag gtcacccatt ggaaacactc gcggaacgtc tcgatgtcca tgatattgaa
     1141 gaacttccaa taatcgtcga gaaattcata gcgcacgatg cctttaatgt gggtgcggac
     1201 gtcgaacgcg gtggaatcgc ccgaagcacc catcagcacg agcgagtctt tgacgatcat
     1261 cttgtcgcgg tacttcttcg cctcatcgga aaaattcttc tcttcgagcg gaaaacccgc
     1321 gggtttcaac cagatattct ggtcgttgaa gaagcgcgtg cggcgtccct ggctgaccag
     1381 catgccgcgc tcctgcgggc cgagcagctt gccttcgacg agtttgacgt tctctttgaa
     1441 catcgcgttg tacgcgggaa aatcaacgcc taagagcatc gcgaagtcaa tatcgccttc
     1501 ttcggtgagc acgaggctga agccgcggcc tgcgggtacg taacctgcaa tcggctgctg
     1561 gctggtcagc accgatttga cgcggtcaaa gtgtttaatg agtgcgatat ctttgcccat
     1621 cggcgtaaaa agaatattgg gattttcctg cgtgtcagag atcaccacca cgtggccggt
     1681 gaagcgctcg acgatgtttt cgcgtaagcc acggttcatg ccgccaatca cagcattgcc
     1741 cagcgtcatg atcagcgctc ccaaaaacaa aatcgcgccg atcaccaggc tcttgccctt
     1801 gtggcgcagc aggttgcgcg cggcgatggt gaacgcgatc ttc
//
LOCUS       DAOQJF010000495         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_54031_length_1842_cov_3.992166, whole genome shotgun sequence.
ACCESSION   DAOQJF010000495 DAOQJF010000000
VERSION     DAOQJF010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54031_length_1842_cov_3.992166"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PLF85_17010"
     CDS             complement(<1..1284)
                     /locus_tag="PLF85_17010"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNE21421.1"
                     /translation="MPTIIKQDDVIESVAGALQYISYYHPPDFIQAMKSAYEKEESRA
                     AKDAMAQILLNSRMSAIGHRPLCQDTGIVSVFVNVGMDVQWQATMTLEDMINEGVRRA
                     YMHPDNVLRASIVNDPAGARKNTKDNTPAVIYTRLVPGNEVEITVAAKGGGSENKAKF
                     AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTADKAMVMAKESLMDPIDIQEL
                     IARGPQNKAEELRITLYNEVNKLGIGAQGLGGLTTVLDVKIKDCPTHAASLPIAIIPN
                     CAATRHAHLTLDGSGPVYLDPPKIEDWPNIEWKAEGAKRVNVNQLKKEDLAAFKPGET
                     LLLTGSILTGRDAAHKRIADLVAKGEKLPDGVDFNNRFIYYVGPVDPVRDEVVGPAGP
                     TTATRMDKFTDMMLEKTGLIGMIGKSERGPQAIEAI"
     gene            complement(1408..>1842)
                     /locus_tag="PLF85_17015"
     CDS             complement(1408..>1842)
                     /locus_tag="PLF85_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE21422.1"
                     /translation="WMHRLVHRKRLYRLVHLVHHRSTNPSPLAAYNFHALEAFLEGIY
                     LVIFTTLVPTHFWVLLGHTFYAMLMNIWWHLGYEFFPAGWATHSVLKWINTSTHHNMH
                     HAKFDGNYSLYFNFWDRIMGTNFPDYEKHYAEVTARRKGASA"
BASE COUNT          362 a          473 c          550 g          457 t
ORIGIN      
        1 gatcgcttcg atcgcctgtg gccctcgctc tgacttgccg atcatgccga tgaggcccgt
       61 cttctcgagc atcatgtcgg tgaatttgtc catgcgcgtg gcagtcgtcg ggcccgcagg
      121 tccaaccacc tcgtcgcgta ccgggtcgac cggaccgacg tagtaaatga agcggttatt
      181 gaagtcgaca ccgtcaggca gtttttctcc cttcgccacc agatcggcga tgcgcttgtg
      241 cgcggcgtcg cggccagtga ggattgaccc cgtgagcagc agggtttcac ccggtttgaa
      301 ggcggcaagg tcttcttttt tgagctggtt aacgttaacg cgtttagctc cctctgcctt
      361 ccattcaatg tttggccagt cttcgatttt cggcgggtct agataaaccg gacctgaacc
      421 atcgagcgtc aggtgtgcat ggcgggtggc cgcacagttg gggataatcg caatcggcag
      481 cgaggcagca tgcgtcgggc agtctttgat tttgacgtcg agcactgtgg tgaggccacc
      541 cagtccctgt gcaccaatac ccagtttgtt cacctcatta tagagtgtaa tgcgcagctc
      601 ctcggcctta ttctgtggcc cgcgtgcgat gagttcctgt atgtcgatgg ggtccatcag
      661 cgattctttt gccatcacca tggctttatc tgctgtaccg ccgataccga tacccaacat
      721 gccgggcggg caccagccag cacccatggt tggtacggtt tttaaaaccc agtcgacgat
      781 gctgtcagaa gggttgagca tagcgaattt cgccttgttc tccgagccgc cgccttttgc
      841 agcgacagta atttcaacct cgttacccgg gacaagccgt gtatagatga cggccggtgt
      901 gttgtccttg gtgttcttgc gcgccccggc agggtcgttg acaatcgacg cgcggagtac
      961 gttgtccggg tgcatatagg cgcggcgtac accttcgttg atcatgtctt cgagtgtcat
     1021 ggttgcctgc cactgcacgt ccataccgac gttgacgaat actgaaacaa tgccggtgtc
     1081 ctggcagagg gggcggtggc cgattgccga catgcgggag ttcagaagaa tctgcgccat
     1141 cgcgtctttg gcggcgcgtg actcctcttt ttcatacgct gatttcatcg cctggataaa
     1201 gtcgggcggg tggtagtaag aaatatactg cagcgcgccg gctaccgatt cgatgacgtc
     1261 gtcttgtttg atgattgtgg gcataggcca gcagcgaatt tttgagttat gttggaaatc
     1321 agattgtagg gaaggttaaa ccacggcggg cacggcgatg gcgtagtctg ccaccgtgga
     1381 tgtgctgagc tcaggttgtc cgactgttta tgcggaggcg ccttttcggc gcgccgttac
     1441 ttcagcgtag tgcttttcgt aatccggaaa attcgtgccc atgatacgat cccagaaatt
     1501 gaagtatagg ctgtagttgc catcgaactt cgcatggtgc atgttatggt gggttgaggt
     1561 attgatccat ttcaaaaccg agtgtgtcgc ccaaccggcg gggaaaaact cgtaacccag
     1621 gtgccaccag atgttcatga gcatcgcata gaaggtgtgc ccgagcagga cccagaagtg
     1681 cgttggcacc agtgttgtga aaatgacgag gtagatgccc tcaagaaatg cctcgagcgc
     1741 gtgaaagttg taagcagcca gcggtgaagg gttggttgat ctgtggtgca caagatgaac
     1801 cagacggtag agccgcttgc ggtgtaccag tcggtgcatc ca
//
LOCUS       DAOQJF010000496         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_54241_length_1839_cov_3.565022, whole genome shotgun sequence.
ACCESSION   DAOQJF010000496 DAOQJF010000000
VERSION     DAOQJF010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54241_length_1839_cov_3.565022"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>1839
                     /locus_tag="PLF85_17020"
     CDS             42..>1839
                     /locus_tag="PLF85_17020"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNE21423.1"
                     /translation="MSEQAATAPAGEIRVALDSGGIAAGAAAIFDELYRLAQDYPSIR
                     VMRVGTPGMCYADPQAEFLLAGRPNLHFGNLTVPAVQRIFADFVLKGHMPQHTRGQFS
                     LLAARRVGHALPQAISSIICCDTMQRGDEDLLSLLKACVEPNVEIIEADDFGFYNRGY
                     ALQFFPGAFTVADLTRDQLASCVHAVVQGKEPEVGKVVRESAQLRLASRNCGVIDPES
                     IEAYEAAGGYAALRKALGRAPTDTVSEVLQSGLRGRGGAGYPTGKKWQLTAAEPGSEK
                     YVICNGDEGDPGAFMDRSLLEGDPHRVLEGLILAGYATGASVGYFYIRAEYPLATARV
                     QLAIDSARAKGYLGKNILGSNFSFEARVRLGAGAYVCGEETALIASIEGKRGSPHARP
                     PYPSVRGLHDAPTTINNVETLASVPEIIVRGGEWYAAIGKGKSTGTKVFAVSGKISRT
                     QLVEVPLGVSVRYVVENLCGGIDRNRQLKAVQTGGPSGGLVPADLLDTPLTYEDMSAL
                     GSIIGSGGMIVLDDSVNMVELMHFYLGFNTDESCGKCAPCRVGGFQLQRLYDKLLAGN
                     GQAEDLKQIERIAHAMQSASLCGLGKAAPSPLF"
BASE COUNT          371 a          527 c          579 g          362 t
ORIGIN      
        1 aagactaccg cactactgca gaacctcgac aggggggagc gatgagcgaa caggcggcaa
       61 ctgccccagc gggggaaatt cgcgtcgccc tggactcggg cggcattgcc gccggtgccg
      121 cggcaatttt tgacgagctt taccggctgg cccaggatta tccctccatc agggtaatgc
      181 gcgtgggtac acccggaatg tgttatgcag acccgcaggc cgaattttta ctcgcgggca
      241 ggccgaacct gcactttggc aacctgaccg tgccggcagt gcaacgcatc ttcgccgatt
      301 ttgttcttaa agggcacatg ccgcagcata cgcgcggcca attttccctc ctcgccgccc
      361 ggcgcgtagg gcatgcgctc ccacaagcaa tcagcagtat catctgctgc gataccatgc
      421 agcggggaga cgaagatctg ctctcgctgc tgaaggcctg cgttgaaccc aacgtcgaaa
      481 ttatcgaggc cgacgacttc ggtttctaca accgcggtta cgcgctgcag ttttttcccg
      541 gtgcgtttac tgtggccgac ctgacccgcg accagcttgc cagttgtgtg catgcggttg
      601 tgcagggtaa agaaccggaa gttggcaagg tggtgcgtga gagcgcacag ctgaggctcg
      661 cgagccgcaa ctgtggcgtg attgatcccg agagcatcga agcctatgaa gctgcaggcg
      721 gttatgcggc gctacgcaaa gcactcggcc gtgctccgac agatacggtg agcgaagtat
      781 tgcagagtgg cctgcgtggc cgcggcggtg cagggtaccc cacgggaaaa aagtggcagc
      841 tgactgcagc agaacccggc agcgaaaagt atgtcatctg caacggtgac gagggtgatc
      901 ccggggcgtt catggaccgc agcctgctcg agggagatcc gcaccgcgtg ctcgaaggtc
      961 ttatactcgc gggttatgcg accggtgcca gcgtcggtta tttctacata cgtgcggaat
     1021 acccgctggc gacggcgcgc gttcagcttg cgatcgattc ggcgcgcgca aagggttacc
     1081 tgggcaaaaa tattctcggc agcaactttt cgtttgaagc gcgcgtgcgg cttggcgccg
     1141 gcgcatacgt ctgcggcgaa gagaccgcgc tcattgcctc gatagaaggc aagaggggca
     1201 gtccccacgc gcggccgccg tacccttcag tcaggggcct gcacgatgcg ccgaccacga
     1261 tcaacaatgt cgaaaccctc gctagcgtgc ccgagattat tgtgcgtggc ggtgaatggt
     1321 atgcagccat cggcaaaggc aagtcgaccg gcaccaaagt ctttgctgtc tctggaaaaa
     1381 tcagccgcac gcaactcgtc gaggtgccgc tcggtgtgag cgtgcgctac gtggtcgaga
     1441 atctctgcgg cggcatcgac cgcaaccggc agcttaaggc agtgcagacg ggtggacctt
     1501 cgggtgggct tgtacccgcc gacctgcttg atacgccact cacatatgaa gatatgtcgg
     1561 cgcttggctc gattatcggc tcgggtggca tgatcgttct cgacgactcg gtgaacatgg
     1621 tcgagctcat gcacttctac ctcggcttca ataccgacga gtcgtgtggc aaatgtgcac
     1681 cctgccgggt agggggcttc cagttgcagc ggctctacga taagttgctc gcgggcaacg
     1741 ggcaggccga agacctgaaa caaattgaaa ggattgcgca cgcaatgcag agtgcgtcgc
     1801 tgtgtgggtt aggcaaagcc gctccctcac cactttttg
//
LOCUS       DAOQJF010000497         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_54856_length_1828_cov_5.530175, whole genome shotgun sequence.
ACCESSION   DAOQJF010000497 DAOQJF010000000
VERSION     DAOQJF010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54856_length_1828_cov_5.530175"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PLF85_17025"
     CDS             <1..966
                     /locus_tag="PLF85_17025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE21424.1"
                     /translation="TWPLFEDVIIYSYVAVVFIQSWLSGTHYTTGMLLLMVGAYMTSA
                     LADIRKVLRAYIVVVVIFAIFGVAEASGRFTYAPIFAKSPYNADGSPVWGWFAIHVTQ
                     VSALLVITYVGIMATKRWVERENLFREMSTIDGLTRLTNRRSFIERSQSEFARARRVP
                     ISGISCIMIDIDHFKKINDTLGHHAGDAVLVQTAGLLLASARQYDEVGRYGGEEFAVL
                     LPETTIEVATQVAERLREKIAAQNITVDGKPVRVTASFGVACFPGEGVETINDLLKAA
                     DAALYKAKHAGRNRVVADARQPRLRQVNKKKSAARKKARNTAGRR"
     gene            1133..1792
                     /locus_tag="PLF85_17030"
     CDS             1133..1792
                     /locus_tag="PLF85_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE21425.1"
                     /translation="MGEISVPALEQVNLSLYEGEFVVLLGPSGSGKSTLLNILGGLDT
                     PTGGRVLFRDRDISTMSEDELTQFRRAHIGFVFQFYNLIPSLTAQENIALVTDIVDDP
                     MDTEQAMGLVGLSDRLGHFPAQLSGGEQQRVAIARAIAKKPDVLLCDEPTGALDFKTG
                     RVVLEAINTVNETLGTTTVVITHNASIADMADRVIEMRDGNIVSDRVNKRRRPVSELS
                     W"
BASE COUNT          386 a          513 c          550 g          379 t
ORIGIN      
        1 acctggccgt tatttgaaga tgttatcatc tacagttatg tggcggtggt gtttatccag
       61 tcgtggcttt cgggcacaca ctacacgacc ggtatgctac tgctgatggt cggtgcatac
      121 atgacctcgg cgcttgccga catacgcaaa gtgctccgcg cctatattgt tgtcgtggtg
      181 atcttcgcca tcttcggcgt tgctgaagcg agcggcaggt tcacctatgc gccgatcttc
      241 gccaagtcac cctacaatgc cgatggatcg ccggtgtggg gttggtttgc cattcatgtg
      301 acgcaggtct cggcactgct ggtgattact tatgtcggca tcatggctac caaacgctgg
      361 gtggagcgag agaacctgtt ccgcgagatg tcaaccattg acggtctgac ccgcctgaca
      421 aaccgccgct ctttcatcga gcgcagccag tcagaatttg cgcgcgcgcg gcgggtaccc
      481 atctcgggaa tttcgtgtat catgatcgac atcgatcact tcaagaagat caacgacacg
      541 ttgggtcacc acgcgggtga cgccgtgttg gtacagacag cgggtcttct gctcgcgagc
      601 gcgcgccagt acgacgaagt gggccgctac ggcggtgaag aattcgcggt gcttctgccc
      661 gagacaacga tcgaggtggc gacgcaggtt gctgagcgct tgcgggaaaa aattgctgcg
      721 cagaatatca cggtcgacgg caaaccggtg cgggtgaccg caagttttgg cgtcgcctgt
      781 tttcccggtg agggcgttga gaccatcaac gatctgctca aggccgccga tgccgctctc
      841 tacaaagcga agcatgccgg ccgtaaccgg gtggttgccg atgcccggca gccgcgcctg
      901 aggcaggtga ataagaagaa atccgctgcc aggaagaagg ccagaaatac cgcaggccgt
      961 agataaccaa atcgcatgac gtcccatgtg cgtgggcaag cgcggtaggt aagagtcgag
     1021 catgtctacc aacgaccgca caccaagggc tcgtaaaaaa tcgccaaggc agccagcccg
     1081 tttgaaccgt accgcggttt ttcaggccga gtcggtcgct aaagtctacc acatgggtga
     1141 gatcagcgtg ccggctctcg agcaggtcaa cctgtcgctg tacgagggtg aatttgttgt
     1201 actgctcggc ccgtccggtt cgggtaaatc gacactgctg aatatactcg gcggtcttga
     1261 tacgccgaca ggcggccggg tgctgtttcg tgaccgcgat atctccacca tgtcagaaga
     1321 tgagctcacg cagtttcgcc gcgcgcatat cggtttcgtc ttccagttct acaacctgat
     1381 acctagcctg actgcgcagg aaaacatcgc gctcgtcacc gatatcgttg acgacccgat
     1441 ggataccgaa caggccatgg ggttagtcgg gctcagcgac cgcctcgggc attttcccgc
     1501 gcagctatcg ggcggggagc agcagcgtgt ggcgattgcg cgcgccattg caaaaaagcc
     1561 cgatgtgctg ctgtgcgatg agccgacggg cgcgctcgat ttcaagaccg gccgcgtcgt
     1621 actcgaagcc atcaacacgg tcaatgagac actgggcacg acgacggtgg ttatcacgca
     1681 caatgcctcg atcgccgata tggcagaccg cgtgattgag atgcgcgatg gcaatatcgt
     1741 ctcagaccgc gtgaacaagc gccgtcgtcc cgtatcagag ctcagctggt gaatacgatc
     1801 aaccgcaaga tctggcgcga aatcggcg
//
LOCUS       DAOQJF010000498         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_54931_length_1827_cov_4.428330, whole genome shotgun sequence.
ACCESSION   DAOQJF010000498 DAOQJF010000000
VERSION     DAOQJF010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54931_length_1827_cov_4.428330"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLF85_17035"
     CDS             complement(<1..542)
                     /locus_tag="PLF85_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21426.1"
                     /translation="MQLLAIVLGLAILFIIQYIANRLMVLSPERLLKAATQVQSCNNL
                     YSRQLLDLNRLSAHSWAETIDYARIVEDWKQATVKILRKFDFLHHAQENNDFRLPMLL
                     LGQKLKVENTEIPDKFVFRIFDSVLQQIAKTTGQSNSQLLKRMGEQLEPNSPLAARFW
                     ELRIFLLGSGRSEIPAPTNPG"
     gene            659..802
                     /locus_tag="PLF85_17040"
     CDS             659..802
                     /locus_tag="PLF85_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21427.1"
                     /translation="MPNQKSEQITRVRVDTELAQPRLIEMMLAREATIVPEVWLTQAN
                     DRA"
     gene            858..1103
                     /locus_tag="PLF85_17045"
     CDS             858..1103
                     /locus_tag="PLF85_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21428.1"
                     /translation="MKPKFFAITLIVTFALGAGLAEAKPPMRDDNGVEVPTSDTKKKP
                     AGSECKSTVECRQHHRCAQSGEKKVCTPPASHLIPPT"
     gene            1236..1688
                     /locus_tag="PLF85_17050"
     CDS             1236..1688
                     /locus_tag="PLF85_17050"
                     /inference="COORDINATES: protein motif:HMM:NF021640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HNE21429.1"
                     /translation="MQENEQEELYLSVYVSAEQAEIAKEIDEYIETKLGHEVIAAKPA
                     PANAPVSIEQLEQDTEDCSFAVIVMAGTTASSREQLLHEIGYCQGTFGCQNVLVLRPD
                     NVPAFANMTGVMYEVFSGENIRAAFPRIRTEIDAAIERFLSDDDIDED"
BASE COUNT          460 a          495 c          468 g          404 t
ORIGIN      
        1 cccgggttag tcggtgcagg gatctccgaa cgccctgaac ccagcaggaa tatgcgtaat
       61 tcccaaaatc ttgccgcgag gggggaattt ggctccaact gctcgcccat tcttttcaac
      121 agctgactgt tgctctgtcc ggttgttttg gcaatctgct gcaacacact atcaaaaatg
      181 cgaaatacaa acttatcggg tatttcggtg ttctctactt tgagcttttg tcccagaaga
      241 agcatgggta aacggaaatc gttattctcc tgcgcgtggt gaaggaaatc gaactttctg
      301 agaattttca ccgtcgcctg cttccagtct tcgactatcc tggcgtaatc gatagtctcg
      361 gcccaactgt gagcactcag tcggttgagg tccagcagct gacgactata cagattattg
      421 caggactgaa cctgagtcgc ggccttaagg agccgttcgg gtgaaagcac catgagacga
      481 ttggcgatat actgtatgat gaagagtatg gccaagccca gaacgatagc cagcagttgc
      541 atcggctatc ggccgccatt gcacccctgc ggcaagcaga tttgttacga gacgaattac
      601 ccctgctttg cgttgacctt ttgtgggctc aacgtgaata acacttgccc cgatcattat
      661 gccgaaccag aaatccgaac aaattacacg tgttagggtt gatacagaac ttgcgcaacc
      721 aaggctgatc gagatgatgc tcgcgcgcga ggcgacgatt gtgcccgagg tgtggttgac
      781 ccaggcaaat gaccgcgcct gaaacccatt tcgaacctgg tggcgcggct ggcgtccaag
      841 taaagaggag ttacactatg aaacctaaat ttttcgcgat cacgcttatt gtaacatttg
      901 cgctcggcgc aggcttggct gaagctaaac cgccgatgcg cgatgacaat ggcgttgaag
      961 tgcccacctc cgatacaaaa aagaagccag ccgggtcaga gtgcaagtct acggtcgaat
     1021 gccggcagca ccatcgctgt gcgcaaagcg gcgaaaagaa ggtctgcacc ccacctgcat
     1081 cgcatcttat tccaccgacg tgagttggca cagggttgct cacgccagaa cagtccgcgc
     1141 cactgccctt gctgaattca gcccctggta aaaaaggcat tccacttttt gcctttaggg
     1201 atacagcgcg actcggattc atttttaggc ttgcgatgca agaaaacgaa caagaagaac
     1261 tttacctaag cgtctacgtc tcggctgagc aagccgaaat tgccaaagag attgacgaat
     1321 atatagaaac aaaactcggt cacgaagtca tcgccgccaa acctgccccg gcaaatgcgc
     1381 cggtcagtat cgaacagctt gagcaagata ccgaagactg ctcgttcgcg gtaatcgtaa
     1441 tggccggcac cactgccagc tcgcgtgagc agctgctgca tgaaatcggc tattgccagg
     1501 gaacgttcgg ctgccagaat gtactggtac tgcgccccga caacgtaccc gcttttgcga
     1561 acatgacggg tgtgatgtac gaggtttttt cgggcgagaa tatcagggcg gctttcccac
     1621 gcattcgcac cgaaatcgac gcggcgatcg aacgctttct gagcgatgat gacatcgatg
     1681 aagattagtc cgaaccagaa ccccgaacac ctgcgcttca actatgagag cacgggtgaa
     1741 ctcaccgttt ccgcgaagtg ctgacaggtc tcaactcccg ctgacatgcc agtctcaacc
     1801 gacctgctca ccgtcctcaa acaaacc
//
LOCUS       DAOQJF010000499         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_55164_length_1823_cov_4.042986, whole genome shotgun sequence.
ACCESSION   DAOQJF010000499 DAOQJF010000000
VERSION     DAOQJF010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55164_length_1823_cov_4.042986"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLF85_17055"
     CDS             <1..789
                     /locus_tag="PLF85_17055"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNE21430.1"
                     /translation="YVSLPALVIKSLRIIPMTPAYFSAALMPWIMFLISAIIFLTLER
                     LKVFNRPTALALMITAGLANTSFVGIPVIEALYGKQYVPVGVIIDQMGTFLLVSLVVI
                     PLVDFLRIGEFSLRVAVRNLFFFPPFLALLLAFVFQYLRPIELVYSVADRLADTLAPL
                     ALVSVGLQWQSSSLRNFAKELSFGLGYKLLLAPLVIWFIYSSAGYIPDLRKIVVLEAA
                     MGPMITGGIIAMQKNLNPPLVAAMLSVGIPLSLLTSLAVKAFFR"
     gene            937..>1823
                     /locus_tag="PLF85_17060"
     CDS             937..>1823
                     /locus_tag="PLF85_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HNE21431.1"
                     /translation="MSKIQKEPQLQMGTAYIPGQTGMYDPQFEKDSCGVGFIVDLKAN
                     KSRTIVEKALQMLNNLEHRGAVGADPETGDGAGILIQMPHDFMKIAAAEAKITLPNEG
                     EYGVAFIFMPQDTGTRKQVENIFEKIVVDEDLEFLGWRKVPVDENVPGYGAKLTLPYV
                     VQAFVGRGKFKGSADDFERRLYLVRRVIDHRIRAELKLNRAQYYVPSFSSHTIVYKGM
                     LLAAQVPAFFKDLKHPEMKSALALIHMRFSTNTFPTWDLAHPFRMIAHNGEINTLRGN
                     INWMKTREAVMESPHYGPDL"
BASE COUNT          380 a          503 c          478 g          462 t
ORIGIN      
        1 tatgtatcgc tgccggcgct ggtgataaaa tcgctgcgca ttatacccat gaccccggca
       61 tattttagcg cggcgctcat gccctggatc atgttcctga tctcggcgat catctttctc
      121 acgctcgagc gcctcaaggt ttttaaccgt cccactgcgc tggcgctcat gattacagca
      181 ggtctcgcga atacttcgtt tgtcggcatc cctgtgatcg aagctctcta cggcaaacag
      241 tatgttccgg taggggtgat tatcgaccaa atgggtacgt ttctgctcgt ctcgctggtt
      301 gtgattccct tggtggattt tctgcgcatc ggtgaattca gtctgcgcgt cgcggttcgc
      361 aacttgttct tttttccacc gttccttgcc ctgctgctag cgtttgtttt ccaatacctg
      421 aggccaatcg agcttgtgta ttcagtagcc gacaggctgg ccgacacgct tgcgccgctg
      481 gcgctcgttt ctgtcgggct acagtggcag tcgagctccc tcaggaattt tgccaaggag
      541 cttagcttcg gcctgggcta caagctacta ctcgcgccgc tcgtgatctg gttcatctac
      601 agttcagcgg gctatatacc tgacctgcgc aagattgtcg tactcgaggc cgctatgggg
      661 ccgatgatta ctggcggtat tatcgccatg cagaagaacc tgaatccccc gcttgtggct
      721 gccatgctct cggtgggaat tcctctgtct ctgcttactt cgcttgctgt gaaggctttt
      781 ttccgctaac gcgcccgctt ttgctcgaaa agtaggcaga ctgttcaggt ttctggcaaa
      841 aaagctgatt ggtgcgtgtt ttgctgtgca ttatattgac gcgatgtcta aaaaccattt
      901 tatgctaggg tttgacccca ttttggggga gggaacatga gcaaaataca aaaagagcct
      961 caattgcaga tgggtacagc ctatatcccc ggacaaacgg gtatgtatga cccacaattc
     1021 gagaaggatt cgtgtggtgt cggcttcatc gtcgacctaa aggcgaataa gtcgcgtaca
     1081 atcgttgaaa aagctctgca aatgctcaac aatctcgagc accgcggtgc cgtcggggct
     1141 gaccctgaaa cgggcgacgg cgccggtata ctcatccaga tgccacacga cttcatgaag
     1201 atcgcggccg ctgaagccaa gatcaccctg cccaacgagg gcgaatacgg tgttgcattc
     1261 atcttcatgc cgcaggacac tggtacgcgt aagcaggtag agaatatttt tgagaagatc
     1321 gtcgtcgatg aagacctcga gtttctcggt tggcgtaaag ttcccgtcga cgagaatgtt
     1381 cccggttatg gcgcgaagct cacgctgccc tatgtggtcc aggcattcgt tggccgtggc
     1441 aagttcaaag gcagcgccga tgatttcgag cgtcgcctgt acctcgttcg ccgggtcatt
     1501 gaccaccgca ttcgcgcgga actgaaactg aaccgcgcac aatactacgt gccgtcattc
     1561 agcagccaca cgattgtcta caagggtatg ttgctcgcgg cacaggtgcc tgctttcttt
     1621 aaagacctca agcaccccga aatgaaatca gcgctcgcgc tgatccacat gcgcttctcg
     1681 accaatacct tcccgacctg ggatctcgct cacccgttcc gcatgattgc gcacaacggc
     1741 gagatcaaca cgctgcgcgg taacatcaac tggatgaaaa cccgtgaggc tgttatggag
     1801 tctccgcact acgggccaga cct
//
LOCUS       DAOQJF010000500         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_56608_length_1799_cov_4.772362, whole genome shotgun sequence.
ACCESSION   DAOQJF010000500 DAOQJF010000000
VERSION     DAOQJF010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56608_length_1799_cov_4.772362"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1156
                     /locus_tag="PLF85_17065"
     CDS             59..1156
                     /locus_tag="PLF85_17065"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNE21432.1"
                     /translation="MRAAFRYGLIAALALPAGACVQNSKALLDEQKAELGRQIFFDTS
                     LSKNSNQSCAGCHSPSAGFSDPSMHASGVSEGSDTSKFGTRNTPAIGYAMFSPPAHFD
                     AEAGTMVGGQFLDHRAASLAEQALKPFLNPVEMANANGDAVVTKIMGRPYAADFRKVF
                     GDDAFSDKSAAYRMIGEALAEFERGTAFGKFDSKFDAVRRGLANFTDEEARGLALFNG
                     KANCAACHPSAGAAPLFTDFTNDNIGVPKNTEIPINKLGDPAYDASFSDRGLGAILDD
                     TAFDGRFKVPSLRNVAITAPYMHNGFFKNLREVVRFYNSACAAGNPDNWDTPEFTGTQ
                     NCKELGDLGLSDDEIDAIVAFLGTLTDGYSN"
     gene            1195..>1799
                     /locus_tag="PLF85_17070"
     CDS             1195..>1799
                     /locus_tag="PLF85_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21433.1"
                     /translation="MRRILSIWAIVSTVAFAQGTKSPQTAEESKKTEIAPDVQTEQDR
                     KIEILSQEVEKLKAGKELFPAAKETGRYGLGPAASKVYEVNQGVSIGGYGEFLYNKNA
                     RTNEAGNDSGQNDKIDSLRQIVYVGYKFNDKWVFNSEIEFEHAGAANVYTEFAAIDYL
                     HDPRLNFRAGLLLIPMGIYNEMHEAPTFLAATRPLTETVIMP"
BASE COUNT          461 a          480 c          480 g          378 t
ORIGIN      
        1 acggcgcgca gacaggtgta aaccagatca atatcagcct cggctatata ttctgaaaat
       61 gcgcgctgct tttcgatacg gtcttatcgc tgcgctcgcg ctgcctgcgg gcgcgtgcgt
      121 tcaaaattcc aaagcgctgc tcgatgaaca gaaagcagag cttggccggc agattttttt
      181 cgacacaagc ctgtcaaaaa actcgaacca gtcgtgcgcc ggctgccact cgccgtccgc
      241 aggtttttca gatccatcga tgcatgcgag cggcgtctct gagggatcag acaccagcaa
      301 attcggtacg cgcaatacac ctgcgattgg ttatgcaatg ttcagtccgc ccgcgcattt
      361 cgacgccgaa gccggcacca tggtaggcgg tcagtttctc gaccaccgcg ctgctagtct
      421 cgcagaacag gctctcaaac cttttctcaa tccggttgaa atggcaaatg ccaacggcga
      481 cgcagtcgtg accaagataa tggggcgacc ttacgctgcg gattttcgca aggtattcgg
      541 cgatgacgcc ttcagcgaca aaagtgctgc ctaccgcatg atcggcgaag cccttgccga
      601 atttgaacgc ggaacggcat ttggcaaatt tgattcgaaa tttgatgccg tacgccgggg
      661 gctggcgaat tttactgatg aggaagcccg cggtttggcg ctcttcaacg gcaaggccaa
      721 ctgcgctgcc tgccatccat cggcaggtgc cgcgccgctt ttcactgact tcacgaacga
      781 caatatagga gttccaaaga atacagagat ccctatcaac aagctaggcg atccggccta
      841 tgatgcatca ttcagtgatc ggggactcgg cgctatcctg gacgatacgg cattcgatgg
      901 gcgcttcaag gtaccgagcc tgcgcaatgt cgccatcacc gcaccctaca tgcacaacgg
      961 ctttttcaag aacctgcgcg aggtggtacg cttctataat agcgcctgcg cggcaggcaa
     1021 tcccgataat tgggacaccc ctgaattcac cggtacgcag aactgtaaag aactgggcga
     1081 cctcggtctg agcgatgacg aaatcgatgc gattgtcgcg tttctgggca cgctgaccga
     1141 tggttacagc aactgaatca ttaatataaa aagaaaagca agaggaaaga ataaatgaga
     1201 agaattttaa gtatctgggc cattgtgagc acggtggcct ttgcccaggg cacgaaaagt
     1261 ccgcaaacag cggaagagag caaaaagacg gaaatcgcac ctgacgtcca gacagaacag
     1321 gatcgcaaga ttgaaatcct gtcgcaggaa gttgaaaagc ttaaagcagg taaggaactg
     1381 tttccagcgg caaaagaaac cggtcgctac ggccttggtc ccgctgcgtc aaaggtttac
     1441 gaagtaaacc agggcgtttc gatcggcggt tatggcgagt ttctctacaa caagaacgcc
     1501 cgcacgaacg aagcgggtaa cgactcgggg caaaacgaca agatcgattc gctgcgccag
     1561 atcgtctatg tcggttacaa gttcaatgac aagtgggtgt tcaactcaga gattgagttc
     1621 gagcatgccg gagctgcgaa cgtctacacc gaatttgctg cgattgatta cctgcacgat
     1681 ccgcggctca attttcgcgc aggactgctg ctgattccca tgggcatata taacgagatg
     1741 cacgaagctc cgacatttct tgcagcgacc cgccctctca cagagactgt catcatgcc
//
LOCUS       DAOQJF010000501         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_56818_length_1796_cov_3.954049, whole genome shotgun sequence.
ACCESSION   DAOQJF010000501 DAOQJF010000000
VERSION     DAOQJF010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56818_length_1796_cov_3.954049"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..791
                     /locus_tag="PLF85_17075"
     CDS             147..791
                     /locus_tag="PLF85_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNE21434.1"
                     /translation="MSRTKYLLLALLATAGIALALNFRKASFIMGGFFAHTGYVIQDW
                     AGHFHDKFHDDDHVHPHEVFNKIIAQNKLQASIWKMTPAVTERKPKALLIMCIDPRLD
                     DNRILGDTRGYYDIIRIPGSVITPEVAEAVELAVVKHAVKLVLVATHTDCAMEKLAQS
                     KDGHAHYPMLSKGINDHALRLKELSHRPEILKRLQKQDVWVIERRIDTATGKFL"
     gene            1044..>1796
                     /locus_tag="PLF85_17080"
     CDS             1044..>1796
                     /locus_tag="PLF85_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNE21435.1"
                     /translation="MKRLMHTLTTVSLMTALLTTAAEAQKRKPAAKPQAEPAAAAAAP
                     ATAEPAQPVATATAPAAAPATEEKWYDTVKISGMVRVRPEVKENFAFDDSQRYNFVGN
                     KVWLTAEKEFKDKTKVVITLQDVRVWGGQNPSITDTGTEQQSTDIREAYVQLKNFFWS
                     PFDLKVGRQKIALGEEMLVGALDWANVGRSFDGARLIWNTSVNDLSIFSTIIQENNSN
                     DLNNANSPKNSPGVYFSGIYDTLKLHKSFWLDT"
BASE COUNT          461 a          490 c          456 g          389 t
ORIGIN      
        1 ggcaaggcaa acatggttgc ggggttactc tgctctaccg cgcgtaaatg gttttcgcaa
       61 atggtacagt ggcgcaataa tagaattata ggtctggtta tattgttttt gtgttgtcgc
      121 ggcctcacca cagcttagaa tggcgcatga gccgcaccaa atacctgtta ctcgcgcttt
      181 tggccacggc gggcattgcg ctggcgctaa actttcgcaa agcctctttc attatgggag
      241 gattttttgc gcacacgggt tatgtcattc aggattgggc aggccacttt cacgacaaat
      301 tccacgacga cgaccacgtg catccgcacg aggtttttaa caagatcatt gctcagaaca
      361 agcttcaggc cagcatctgg aaaatgactc ccgcggttac cgaacgcaag ccgaaagcac
      421 tgctcatcat gtgcattgac ccgcgcctcg acgacaaccg tatcttgggc gacacgcgcg
      481 gttattatga cattattcgt attcccggct cggtgatcac tcccgaagtt gccgaagccg
      541 ttgagctggc ggtggtaaag cacgcagtca aactcgtgct ggtcgcaacg cataccgact
      601 gtgcaatgga aaaactcgcg caatcaaaag atggccatgc acactaccca atgctgtcca
      661 agggtatcaa cgaccatgcg ctgcgcctga aagaattatc gcaccggccc gaaattctca
      721 aacgcctgca aaagcaggat gtctgggtaa ttgaaagaag aatcgatacc gcaaccggca
      781 agtttctgta aaagtgcccc tattccccct ttactgcctg tcgggctcca catgacgagc
      841 atctgtaatc tttttgcatg atacccggga aattcggccc cggttctcat ttgttgccag
      901 tgcataagcc ggcagagaat tcggcccgcc cgttacctgc agaagcaaac cggaatatgt
      961 accctttctt gatctgaatt aaggaaattc ctgagcagct ggtctaaggg acaccgtatt
     1021 ggagattatg gataggagga aacatgaaaa gattgatgca tacattgacg accgtctctc
     1081 tgatgacggc cctgctgacc accgcggccg aggcccagaa gcgcaaaccg gctgcaaaac
     1141 cgcaggcaga accagctgca gcagccgctg caccggcaac ggctgaaccg gcccaaccag
     1201 tggcaacggc gactgccccc gctgcggcac ccgccaccga agaaaaatgg tacgacaccg
     1261 tgaagatttc gggcatggtg cgcgtacgcc ccgaagtgaa agaaaacttt gccttcgacg
     1321 acagccagcg ttacaacttc gtcggcaaca aggtctggtt aactgccgaa aaggaattca
     1381 aagacaaaac aaaggttgtg attaccctgc aggacgtgcg tgtctggggc ggtcaaaatc
     1441 ccagtattac cgataccggt accgaacagc aatccactga catccgtgaa gcgtatgtgc
     1501 agctcaagaa ttttttctgg tctccttttg atctcaaagt cggccggcag aagattgctc
     1561 tgggcgaaga gatgcttgtc ggagctctgg actgggccaa cgtcggccgc tcattcgacg
     1621 gcgcacgcct gatctggaac acctcggtga acgacctgag catcttctcg accatcattc
     1681 aggaaaataa ctccaacgat ctcaataatg ccaatagccc gaaaaattcg ccgggtgtgt
     1741 acttcagcgg tatctatgac acgctgaaac tgcacaagtc gttctggctc gacacg
//
LOCUS       DAOQJF010000502         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_57638_length_1783_cov_3.633102, whole genome shotgun sequence.
ACCESSION   DAOQJF010000502 DAOQJF010000000
VERSION     DAOQJF010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57638_length_1783_cov_3.633102"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLF85_17085"
     CDS             complement(<1..244)
                     /locus_tag="PLF85_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE21436.1"
                     /translation="MARTQEQRRQLTLQKLHEATIECIVTHGFSRLTTIEIARVAGVS
                     QGALFRYYPTKTAAVVGAAGYLFAKVTNDFSALLGSP"
     gene            450..1061
                     /locus_tag="PLF85_17090"
     CDS             450..1061
                     /locus_tag="PLF85_17090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE21437.1"
                     /translation="MSVKLYEALVEQSPIMIWRAGTDGLCNYFNKTWLNFTGKKLEEE
                     LGDGWAKGVHDENLQACMDYYRSNFEQRNIFEMKYRLMRADGQYRWILDRGCPYYDDA
                     DNFAGYIGSCIDITERHTLDLEKEKIYKATVRGTNHILRNMLNQLQGVEMACEDHPEL
                     HKIVAPEISSILKEATGLIERLSSVENISEQNIMAAVMPKGKV"
     gene            1058..>1783
                     /locus_tag="PLF85_17095"
     CDS             1058..>1783
                     /locus_tag="PLF85_17095"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE21438.1"
                     /translation="MNIRTISAWAVEQFYLRFSQLGRLHPVWNPLAQGVQVLTDIPYL
                     PSGSSFHTLDIYRPANLRGHAPVVLYIHGGGFSMLSKESHWIMALMFARSGYVVFNIN
                     YTLAHEAPFPAAIEDSLHALKFVVKNAGLYKGDASEIIVAGESAGANLALSVGLAASH
                     DEVANSLAHEVFTLAPRIKALMLGCGLFEVGQIDRFRGKIPDAFMPVLEHISETYIGP
                     TTGNDIQRPLANPLSLLESGEKMV"
BASE COUNT          447 a          465 c          485 g          386 t
ORIGIN      
        1 cgggcgaacc gagcagcgcg ctaaaatcgt tggtcacctt ggcaaagagg tagccggcgg
       61 cgccgaccac cgcggcagtt ttagtcgggt aataacgaaa cagcgccccc tgtgaaacgc
      121 cggcgacacg ggcaatttcg atggtggtaa ggcgactgaa accatgggtc acaatgcatt
      181 caatggtcgc ttcatgtagt ttttgcaggg tcagctgccg gcgttgctcc tgcgtgcggg
      241 ccatgggcga ctctggattt ttatatatag tgactactca ctttttattt aaccacagaa
      301 aaccagggag cgaacagcct taatcaacaa tacccgtcat ggaagtcaaa gacccgcggg
      361 gtgatagcac tttacgggtc gatggcccgg gattcagggg tagccctgat ggcaatacta
      421 cgtgtctgcc cgtcgcacgg atctaaccga tgagcgtcaa gctctacgaa gcactggttg
      481 aacaatcgcc gatcatgatc tggcgcgccg gcaccgatgg cctgtgcaac tatttcaaca
      541 agacctggct caatttcacc ggcaagaaac tggaagagga attaggcgac ggctgggcaa
      601 aaggtgttca cgacgaaaac ctgcaggcat gcatggatta ttaccgcagt aattttgagc
      661 agcggaacat atttgaaatg aagtaccgcc tgatgcgggc agatggccag taccgctgga
      721 ttttggaccg gggatgcccc tactacgacg atgccgacaa ctttgccggg tacattggca
      781 gttgtatcga cataaccgaa agacacacac tggatctgga aaaagagaaa atctataaag
      841 cgacagtgcg gggtaccaac cacatactga gaaacatgct caaccagctg cagggcgttg
      901 aaatggcgtg cgaagaccat cccgaactgc acaagatagt cgcaccggaa atttcttcta
      961 tacttaaaga ggctactggt ctgattgaaa gactttcttc cgtagaaaat atcagcgaac
     1021 aaaatattat ggcagcagtc atgccaaaag gtaaagtatg aacattcgca cgatttcggc
     1081 atgggccgtt gaacaatttt acctccgctt cagccagctg ggccgactgc acccggtatg
     1141 gaacccgctt gcgcagggtg tgcaggtctt aaccgatatt ccctacctgc cctctggctc
     1201 gagctttcac actctcgaca tctatcgccc ggcaaatctc aggggccatg cccccgtggt
     1261 actctacatc catggcggcg gatttagcat gctttcgaaa gagtcacact ggatcatggc
     1321 attgatgttc gcgcgctcag gttatgtcgt gttcaacatc aactataccc tggcgcatga
     1381 ggcgcctttt cccgcggcga tcgaagattc gctgcacgcg ctgaaatttg tcgtcaagaa
     1441 cgcggggctc tacaagggcg atgcgagtga aattattgtc gcaggcgaat cggccggcgc
     1501 aaacctcgca ctttccgtgg ggctcgcggc aagccatgac gaggttgcca acagcctggc
     1561 gcacgaggtg ttcacactgg cgccgcgtat caaggcgcta atgctcggct gcggactgtt
     1621 tgaagtcggg cagatcgacc gcttcagggg aaaaattcct gacgcattca tgccggtact
     1681 cgagcatatc tcggagactt atattggtcc cacgactggc aatgatatac aacgtccgct
     1741 ggcaaacccg ctctcgctgc ttgaatcagg tgagaagatg gta
//
LOCUS       DAOQJF010000503         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_58034_length_1777_cov_4.433798, whole genome shotgun sequence.
ACCESSION   DAOQJF010000503 DAOQJF010000000
VERSION     DAOQJF010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58034_length_1777_cov_4.433798"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..881
                     /locus_tag="PLF85_17100"
     CDS             24..881
                     /locus_tag="PLF85_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21439.1"
                     /translation="MPANNLQLLYNRLERSFAAAEETFLHAEKIRHELKSDYETLLAA
                     VQAGKHDMARYPQAQLARRLRLIETLLKDLQLRQAGEQRSFHALGFMLSRLRLRLASE
                     ILEPRLYGKIRAALTEEENLATLREKYPDADAVLTEPAPTKQPKKCKNIRLLLVGVNS
                     LYYAIPVRKILKKTMPGPVEEKLRSTGFTALDLPGNRLNSPEMPLAIAFTDIAGHRRL
                     LYCDEYFTPVEIPQRVLRRMVVWAPPADRFGADFRPQVQLYGRRFFVYGARLSYTVRP
                     GERKHPAER"
     gene            898..>1777
                     /locus_tag="PLF85_17105"
     CDS             898..>1777
                     /locus_tag="PLF85_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21440.1"
                     /translation="MRIFIEHKADILPVVERGRIVGQLDRMSIMEALSEIRPEGCLLA
                     LKEAESTEELLRRLDSSEAGGLPTVNRAYEFNALWGRAEILQAQGKIPQVKIWQPETA
                     STPAVETVAKDEPTSAVFAAATQEPVTNLSNLSELAARAAVIDKPIHGEKHPDPKPAA
                     PGATITIVREVPAVAVRSAPRDAAEEPATETASVRSPVEKFHRELERGRLAINTLAAL
                     ELPLMACDGGGSELFHNRAFGDLRRRFAHSLDSGELVKKAKDAIADSALKGDLDIDRP
                     VKLRANVTGHGIFCKAI"
BASE COUNT          421 a          526 c          498 g          332 t
ORIGIN      
        1 aaaattttct ctgaagaaaa acgatgcccg ccaataacct gcagctgctc tataaccgtc
       61 tcgaacgctc attcgctgcg gctgaagaaa cattcctgca tgcagaaaaa atacgccacg
      121 aattgaaatc cgattatgaa acgctgctgg ctgcggtaca ggccggaaag catgacatgg
      181 caaggtatcc gcaggcgcaa ctggccaggc gcttgcggct gatcgagacg ctgctgaaag
      241 acctgcagct gcgacaggca ggcgaacagc gcagcttcca tgcgctgggt tttatgcttt
      301 cgaggctacg cctgcgactg gcgtcggaaa tactcgagcc aaggctctat ggcaagatac
      361 gggcagcgct gacagaagaa gagaacctgg ccaccctgcg ggagaaatac ccggacgctg
      421 acgccgtgct gactgagcct gccccaacaa agcagccgaa aaaatgcaaa aacatccgcc
      481 tgctgctggt cggcgtgaac agcctgtact atgccatacc ggtgcgaaaa attctgaaaa
      541 aaacgatgcc gggtcccgtg gaagaaaagc tgcgctcgac aggttttaca gcactggacc
      601 tgcccggtaa ccggctaaac tcccccgaaa tgccattggc aatcgctttt accgatattg
      661 ccgggcacag gcgcctgctc tactgcgacg aatattttac gccggttgaa ataccccaga
      721 gggtgctgcg ccgcatggtc gtctgggccc ctcctgcaga ccgcttcggc gctgatttca
      781 ggccgcaggt tcagctctac gggcgccgct tttttgtata cggcgcacgt ttgagttaca
      841 ctgttcgccc cggtgagcgc aagcaccctg ctgaacgttg acactctgca tcgcctgatg
      901 cggatcttta tcgaacacaa ggccgatatc cttccggttg tggagcgcgg gcgtattgtg
      961 ggccagctcg accgcatgtc catcatggaa gccctgagtg aaatcaggcc agagggctgc
     1021 ctgctcgctc tcaaggaagc agaaagtacc gaagaactgc tacggcgtct cgacagctca
     1081 gaagccggcg gcctgccgac ggtgaaccgg gcttatgaat ttaatgcgct gtggggccgg
     1141 gcagaaattc tgcaggcgca gggcaaaatt ccgcaggtga aaatctggca acccgaaact
     1201 gcttcaacgc cggctgtcga aacggtcgcg aaagacgaac caacttcagc agtttttgct
     1261 gcggccacac aggaaccagt aacaaacctc tctaacctgt cagaacttgc cgcgcgcgca
     1321 gcagtgatcg acaagcctat tcatggcgag aagcatcccg atccaaaacc tgctgcacct
     1381 ggtgcgacca tcacgattgt ccgggaggtc ccggcggtcg ctgtacgcag cgcaccgcgc
     1441 gacgccgcag aagagccggc aacagagact gcgagcgtgc gttcacctgt ggaaaaattc
     1501 caccgcgaac tggaacgtgg ccgccttgcc atcaataccc tcgccgcgct tgagctgccc
     1561 ctaatggcat gcgatggcgg cggctcagaa ctttttcaca accgggcttt tggcgacctg
     1621 cgccgccggt tcgcacattc actcgacagc ggcgaactgg tcaagaaagc aaaagatgcc
     1681 atcgctgaca gcgcgctcaa gggcgatctc gatattgacc gcccggtgaa gctgcgcgcc
     1741 aacgtcacgg gccatggcat tttctgcaag gcgattc
//
LOCUS       DAOQJF010000504         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_58192_length_1775_cov_3.312209, whole genome shotgun sequence.
ACCESSION   DAOQJF010000504 DAOQJF010000000
VERSION     DAOQJF010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58192_length_1775_cov_3.312209"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLF85_17110"
     CDS             <1..439
                     /locus_tag="PLF85_17110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE21441.1"
                     /translation="DDGSMIDDWVAVKLKRGELTDFFHSVRKSARSGDPYILHDGVDL
                     AAAYGTEVPAQGGRAWSGYNTKWGLHVFEEKGGVRHLYAHLSKTHIRFGQIRVMTAGE
                     VIGRVGMTGNTTGPHLHYGILSLRNQRFINPYHYRFMNGTELW"
     gene            433..909
                     /locus_tag="PLF85_17115"
     CDS             433..909
                     /locus_tag="PLF85_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21442.1"
                     /translation="MVKLLIVIFSGFLEPGSIKSKPIPPGKTIYLDIRRLPKTTNLAS
                     NFGDTLAFNKLGIEIQEILLMAEDQYPYVLVGDHPRAAFKPRDINDMPDLTVRLADIY
                     LQIAYPWPELGELADEKTRVFDGATFLRMKLRFKQDGRAYEKEILFWHGYPPGTAP"
     gene            complement(1106..1531)
                     /locus_tag="PLF85_17120"
     CDS             complement(1106..1531)
                     /locus_tag="PLF85_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE21443.1"
                     /translation="MIFWDSSAIVPLLVNEPKTKEAHAILKKDRNLTVWCLTYLEATS
                     ALNRRLRTDEIHFGHFRAGEERLKKLTHAWDQILFSDRLIQLSARLLRTHPLRTADSL
                     QLAAALIAGGNDPSQVNFFSYDDRLNEAAEREGLRVLVV"
     gene            complement(1528..>1775)
                     /locus_tag="PLF85_17125"
     CDS             complement(1528..>1775)
                     /locus_tag="PLF85_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21444.1"
                     /translation="RQVRQGETVIILDRGIPVARFEPIESWDDETYHLMAVRLTKAGD
                     LLPRKAKLGDDFFDLPLPEDKKGSVREALIAERREGR"
BASE COUNT          413 a          448 c          469 g          445 t
ORIGIN      
        1 agacgacggt agcatgattg acgattgggt ggcggtaaag ctgaagagag gcgagttgac
       61 cgattttttc cattctgtgc gcaaatcggc acgttcaggt gatccttaca tcttgcatga
      121 cggtgtagac ttggcggcag cttacggtac ggaggtgcct gctcagggtg gtcgcgcttg
      181 gtctggctac aatacaaaat ggggcctgca cgtgttcgaa gaaaagggtg gcgtccgaca
      241 cttgtacgcg catttatcaa agactcacat tcggtttgga caaatcagag tgatgactgc
      301 tggcgaagta atcggtcgtg ttggtatgac cggcaacacc actggcccgc atctgcatta
      361 cggaattttg agtctccgga accaacgttt cattaatcct taccattatc gattcatgaa
      421 cggtacagaa ttatggtaaa gctccttata gttatttttt ccggtttcct tgaacctggt
      481 tcaatcaaat ctaagccgat cccccccggt aaaactattt acctggatat caggaggctg
      541 cccaaaacta ccaatttagc gagtaatttt ggagacacgc tcgcctttaa caagctgggc
      601 attgagattc aggaaattct actgatggct gaagaccaat acccatacgt gttggttggg
      661 gatcacccga gggctgcttt taaacccaga gatattaacg atatgccaga tctaactgtt
      721 cgtctggctg atatctatct gcaaattgct tatccttggc cagaattggg cgaacttgcg
      781 gatgaaaaga cgcgcgtatt tgatggggct actttcttgc gcatgaagtt gcgcttcaag
      841 caagacggac gagcatatga aaaagagata ctgttttggc atgggtatcc ccctggtact
      901 gcaccttagc ataccttatt gaatcctctg aataatgact ccgaccaaat caatatcgcc
      961 agaccatatg actcgcgaag tctctgtcgt gagcacagcc agccatgatc cgactacctg
     1021 ctactctgcc ggtggctcgc tgaacgtttg gctaaacggc gcgagttttc tgggatgatc
     1081 ttgctgggcc gtgtgatatc ttatttcaaa ccaccagcac ccgcaacccc tctcgctcag
     1141 ctgcttcatt cagccggtca tcgtaactaa agaagtttac ctgcgacggg tcattaccgc
     1201 ctgcaatcag cgcagccgcg agctgcaatg agtcggccgt gcgcagcggg tgggtgcgca
     1261 gcaggcgggc agagagctgt atgaggcgat cggaaaagag aatctgatcc caggcatgtg
     1321 tgagcttttt caggcgctct tcgcccgcgc gaaaatggcc gaagtgaatt tcgtcggtgc
     1381 gcagccgccg attcagtgcc gatgtcgctt cgaggtaagt gaggcaccag accgtcaggt
     1441 tacggtcttt cttcaatatt gcatgcgcct ctttagtttt gggttcgttg accagcagtg
     1501 gcacaatcgc cgagctatcc caaaagatca tcggccttca cgccgctcgg cgatcagtgc
     1561 ctcgcgcacc gaaccctttt tgtcttcggg cagaggcaaa tcgaaaaaat cgtcaccgag
     1621 ttttgccttg cggggcagca agtcgccggc tttggtcagt ctcacagcca taaggtggta
     1681 ggtttcgtca tcccatgatt cgatgggctc aaagcgcgcg acgggaatgc cgcgatcgag
     1741 aatgatgacc gtttcgccct gtcgcacctg ccgca
//
LOCUS       DAOQJF010000505         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_58518_length_1770_cov_4.014577, whole genome shotgun sequence.
ACCESSION   DAOQJF010000505 DAOQJF010000000
VERSION     DAOQJF010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58518_length_1770_cov_4.014577"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLF85_17130"
     CDS             complement(<1..536)
                     /locus_tag="PLF85_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNE21445.1"
                     /translation="MKKLQENFMFRLRNVAGLFTGLLVVSVSPLMASEADLKIPDLAS
                     KTFFGSLDGRTLLMIGLATCVLGLIFGLVEFIRVKKLPVHKVMLEVSELIYETCKTYM
                     ITQGRFLAILWIFIAAIMIYYFGFLAQGGIDAAGKAIHGYGVAKVVAILGCSVVGILG
                     SYGVAWFGIRINTFANSRT"
     gene            764..>1770
                     /locus_tag="PLF85_17135"
     CDS             764..>1770
                     /locus_tag="PLF85_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21446.1"
                     /translation="MSKYLSAKDLDFIKTIDSFRDTRSSHVPANRLEDVRKAARKFRS
                     ELLETGTVPYFRAATLVNVPYPTRYAFFGVYNDSTFLTPVIHITNRLFLIRFKTDGGV
                     KTLLFSPSDIMANAETRFFKRFAGGFIQKLQGDDMRQIKDTAQKLVAPIEHTVEEWLP
                     LIGLKPEEVDYISYDHLHTQDLRNWLGTNGNPGYFPNAKLLVMRQEWESATGLLPPQK
                     DWYCPNGIAGVDPQKVVLLDDDVLLGPGLALVRTPGHTMGNHSLVVNTPAGVFVSSEN
                     GVSADSYAPLKSRNNEIRAYAEKTGMEVILNGNTQESGIEQYISMVMEKEIAGPAQQN
                     P"
BASE COUNT          454 a          512 c          466 g          338 t
ORIGIN      
        1 gtacgcgagt tggcgaacgt gttgatacgg ataccgaacc aggccacgcc gtagctgcct
       61 aaaataccga caactgagca accaaggatg gcgacaactt ttgcaacacc atagccgtga
      121 atcgccttac ctgcagcgtc aataccgcct tgcgcgagaa agccaaagta gtagatcatg
      181 atcgcagcga taaaaatcca caggatcgcc agaaagcggc cctgcgtgat catgtaggtc
      241 ttgcaggttt cgtagatcag ctctgaaact tcgagcatga ctttgtgcac cggaagtttt
      301 ttgacgcgga taaattcaac caggccaaag atgagaccca gcacgcaggt cgcgaggccg
      361 atcatcagca gcgtgcggcc gtcgagcgag ccaaaaaagg tcttactggc cagatcggga
      421 attttgaggt cggcttcgct tgccataaga ggcgaaacag aaaccaccag caggccggtg
      481 aataggccgg ctacgtttcg tagtctgaac atgaaattct cctgaagttt tttcatcggg
      541 gaaagatgtc cgggtaccct cggggcaaaa aacagggggt aaagcataaa aggcgcgtaa
      601 catggctgaa tagttaatca cttggcggag tgtttccggt cacgctggta gttaccaaag
      661 ggtacattga accgaccccg cagaaaacta taactaattg aatagttttc gatgagtcgt
      721 cagacggttt tagtacctac caaacaggcg aaaggagccc catatgtcca aatatctcag
      781 cgccaaagat ctcgacttta tcaaaaccat cgactcgttc agagataccc gcagctcgca
      841 cgtgccggca aaccgccttg aagacgtgcg caaggccgca cgcaagtttc gcagcgagct
      901 gctcgaaacc ggcacggtgc cctattttcg cgcggccacg ctcgtcaacg tgccctaccc
      961 cacgcgttat gccttcttcg gggtctacaa cgattcgacg ttcctgacgc ccgttatcca
     1021 tatcacgaac cgcctgttcc tcattcgctt caagaccgac ggtggagtga aaaccctgct
     1081 gttctctccc agcgacatca tggccaatgc cgaaacacgc ttctttaaac gcttcgcagg
     1141 gggattcatc cagaaactgc agggcgacga catgcgccag atcaaagata ctgcgcagaa
     1201 gctcgtcgcc ccgatcgagc acacagtcga agaatggctg ccgctcatag gccttaaacc
     1261 cgaagaggtg gactatatca gctacgacca cctgcacaca caggacctgc gcaactggct
     1321 cggcacgaac ggcaaccctg gctattttcc caacgctaag ctgctcgtca tgcgccagga
     1381 atgggaatcg gcgaccggcc ttctgccgcc gcagaaagac tggtactgcc ccaacggcat
     1441 cgcgggcgtc gacccgcaga aggtcgtgct acttgacgac gacgtgctgc tcggccccgg
     1501 cctggcgctc gtccgcactc cgggccatac gatgggcaac cactcactgg tcgtaaacac
     1561 gccagccgga gtttttgttt cgagcgagaa cggcgtctcg gcagacagtt acgctcccct
     1621 caagtcgcgc aacaacgaga ttcgcgccta tgccgagaag accggtatgg aagtcatttt
     1681 gaacggcaac acgcaggagt cgggcatcga gcagtacatc tcgatggtga tggaaaaaga
     1741 aatcgccggc cccgcgcaac agaaccccga
//
LOCUS       DAOQJF010000506         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_59080_length_1762_cov_3.477446, whole genome shotgun sequence.
ACCESSION   DAOQJF010000506 DAOQJF010000000
VERSION     DAOQJF010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59080_length_1762_cov_3.477446"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PLF85_17140"
     CDS             <1..1276
                     /locus_tag="PLF85_17140"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21447.1"
                     /translation="SMLLLYAATWFGYGLLRRSAQYLGLVLVAWSLLLLFAPLALTAR
                     LYFINLQVTVVLAILLLFLMVYALKKGRGEIWILAVGTSFLALGSLAAVLRTLHLIDL
                     QGLEFIGFMLFLATQTSQSVVELTRIRRRQQVLVSENKAALGRLSLFVPRVHLSKVSR
                     RWHEPIKPGKFYHTEACIMFLRIEPDNSSEMAADDLFDLHADFAEEVANFAGRFGGVV
                     DRISVGRYILSFNDDPAIALQLAVQMRLQVRQWEAALDRYILFRCGIHYGSAVWGLYG
                     SPERWSGGYMGDTVNVAARLETLCARYQTSILMSQDAYFQSAHFDEYLTRMLEPVKLK
                     GKDDHIFVYEVLAGLPDERIALIKETLPFFGRGLQAFLNKDFSRAIGFFEKVTALNSG
                     DFSANLYLSRARKLAQQETDETWNPIEALNRK"
     gene            1279..>1762
                     /locus_tag="PLF85_17145"
     CDS             1279..>1762
                     /locus_tag="PLF85_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21448.1"
                     /translation="MIRSILIALLFVLNLPLLAQVTERVYDSEKARLESLSGTWQLYC
                     EDGLPSSAEAVTVPHTIHVPGTWRGYSCPQGKLPGTGRHRFHLVITGKMRPYGLALYF
                     PIAGTSMIAYWNGYKIHEAGDVAAGKPGFRTATVPIAFSERNDLVIDVANFDDRYGGL
                     W"
BASE COUNT          358 a          459 c          478 g          467 t
ORIGIN      
        1 atccatgctt ctgctgtatg ctgcgacgtg gtttggatac ggtcttttgc ggcgcagcgc
       61 gcagtacctg gggttggttc ttgtagcgtg gtctttattg ctcctgtttg ccccgcttgc
      121 gctcacggcg cggctttatt ttatcaacct gcaggttact gttgtcctgg cgatactgct
      181 gctctttctg atggtctacg cgctgaaaaa aggccggggc gaaatctgga tattggcagt
      241 cggaacctcg ttccttgcat tggggtcgct ggccgcagta ctgcgcacac tccacctgat
      301 tgatttacag ggccttgaat ttatcggctt catgctcttt ctcgcgacgc agacaagcca
      361 gtcagtggtg gagttgacac gaatcaggcg acggcagcag gttctcgtaa gtgaaaataa
      421 agccgcactc ggtcgactta gcctctttgt gccgcgcgtc cacctcagca aggttagccg
      481 gcgttggcat gagccgatca aacccggaaa attctaccat actgaagcct gtattatgtt
      541 cttgcgtatc gagccagata atagctctga aatggcggcc gacgaccttt ttgacctgca
      601 cgcagatttt gcagaggagg tagcaaactt cgcgggtcgt tttggcggcg ttgtggatcg
      661 catttccgtc gggcgttata ttctctcctt caacgacgat ccggcaatcg cgttgcagct
      721 tgctgtacag atgcgcttgc aggtgcgtca atgggaagcc gcgctcgatc ggtatatact
      781 ctttcgttgc ggcattcact acggttctgc agtctggggt ctctatggtt cgcccgaaag
      841 gtggtcaggt ggctatatgg gtgatacagt gaatgttgct gcccggttgg aaaccctctg
      901 cgcacgatac caaacatcta ttctcatgag ccaagacgcc tattttcagt ccgcgcactt
      961 cgatgagtac ctgactcgta tgctcgagcc cgtgaaattg aagggcaagg atgatcatat
     1021 attcgtttac gaagttctgg ctggcttgcc tgatgagcgt attgcactca tcaaggaaac
     1081 attgcccttt ttcggccggg gtctacaggc ctttctcaac aaagatttct cccgtgccat
     1141 cggttttttt gaaaaagtca ctgcgttgaa ttccggcgat ttttctgcga atctctatct
     1201 atcgcgtgcc cgcaagctgg cgcagcagga aaccgatgaa acctggaatc ccatcgaagc
     1261 gctgaacagg aaatagcgat gatccgatct attttgattg cgcttttgtt cgttctcaac
     1321 ctgccactgt tggcacaggt tacagaacgt gtatacgatt cagagaaagc tcggctcgag
     1381 tcgctgagcg gaacctggca gctttactgt gaagacggtt tgccctccag cgccgaggca
     1441 gtcaccgtcc ctcacacgat acacgttcca ggtacgtggc gcggctacag ttgcccgcag
     1501 ggaaaattgc cgggtacagg ccgccaccgc tttcatttgg tcataacggg aaaaatgcgg
     1561 ccctatggcc ttgcgctcta tttcccaatt gctggcacga gcatgatcgc gtactggaac
     1621 ggttataaga ttcacgaggc cggtgatgtt gccgcgggca aaccaggatt tcgcactgct
     1681 acagtgccca tcgccttttc ggagcgtaat gacctcgtta tagatgtcgc caacttcgac
     1741 gaccggtacg gcggtttgtg gc
//
LOCUS       DAOQJF010000507         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_60186_length_1744_cov_5.458259, whole genome shotgun sequence.
ACCESSION   DAOQJF010000507 DAOQJF010000000
VERSION     DAOQJF010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60186_length_1744_cov_5.458259"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLF85_17150"
     CDS             <1..478
                     /locus_tag="PLF85_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21449.1"
                     /translation="KQRPAPLACRWLNARASLFAVIKINAQREARAVAHEMERALGGQ
                     LITLLPLSESAGSQDSFSGQAFYLALEKRTNAFSRLNGSAKAENLRLEADDMARDRNL
                     LSYFAALMLTDTPYQTVHLLGFSGLAETEYETFDGLYHRSMRKLEAADTALVEKIR"
     gene            564..953
                     /gene="gcvH"
                     /locus_tag="PLF85_17155"
     CDS             564..953
                     /gene="gcvH"
                     /locus_tag="PLF85_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNE21450.1"
                     /translation="MANIPADLKYTKEHEWVKISGNKARVGITDYAQGALGDVVYIDF
                     PKAGAEIKVMAVAGTIESVKAVSEIYQPLSGKVVVINSDLNKSPALVNTDPYNAGWLF
                     EIEGISEAEAANLLDAAGYDAYLKTLG"
     gene            1100..1432
                     /locus_tag="PLF85_17160"
     CDS             1100..1432
                     /locus_tag="PLF85_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HNE21451.1"
                     /translation="MANYFESEDLKKFGNIGRVNKELADKFFDYYNSTMKAGALSERE
                     KALIALAVAHAMKCPYCIDAYTSTCLEKGADEAQMSEAVHVAAAMQAGIALVHSVQMM
                     NHIDNASI"
     gene            1429..>1744
                     /locus_tag="PLF85_17165"
     CDS             1429..>1744
                     /locus_tag="PLF85_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE21452.1"
                     /translation="MKLNSAEQLRLLGAQAEAFEARAAASGFAGLKPAELTTFQINVG
                     RWCNQACRHCHVDASPQRTEMMDRETLEKCLHIITSVPSITTVDITGGAPEGHPLFRE
                     FVT"
BASE COUNT          434 a          500 c          475 g          335 t
ORIGIN      
        1 caaacagcga cctgctcctt tagcgtgtcg gtggctgaat gccagggcat cactcttcgc
       61 cgtcatcaag atcaacgcgc agcgcgaagc gcgtgcggta gctcacgaaa tggagcgcgc
      121 attgggcggc cagctcatca cgttactgcc gctttccgag agcgcagggt cacaggacag
      181 cttctcgggc caggcttttt acctcgcact ggaaaagcgt acgaacgcat ttagccggtt
      241 aaacggcagc gccaaagcag aaaacctgcg gcttgaagca gatgatatgg cgcgcgaccg
      301 caacctgttg tcttactttg ctgccctgat gctcacagac actccctacc aaactgtaca
      361 tcttctcggc ttctcgggcc tcgcagagac cgaatacgag actttcgacg gtctctacca
      421 ccgctcgatg agaaaactgg aagccgcaga cactgcgctc gtagaaaaaa tccggtaatc
      481 tttcgcggta caggccggaa tccttaccgg ttattcgggc gcaaagatat ttactgccta
      541 tgagtgggcc acaccctgcc cccatggcaa atattcccgc ggacctcaag tacactaaag
      601 aacacgaatg ggtaaagatt tcaggcaaca aggcccgggt cggtattacc gactatgcac
      661 agggcgccct cggcgacgtt gtctatattg actttcccaa agcaggtgca gaaattaagg
      721 tcatggcggt agcgggaacc atcgagtcgg tgaaagccgt ctcggaaatc taccagccac
      781 tttcgggaaa ggtggtcgta atcaacagcg acctgaacaa gagccccgcc ctggtcaata
      841 ccgaccccta taacgcggga tggttgtttg aaatcgaagg catctctgaa gctgaagcag
      901 cgaacctgct cgatgctgca ggctatgacg cctacctgaa gacactcggc tagctgatgc
      961 accaccgccc tggcaaaaca gaacctatgc cggggtaaaa tatcgccagc tgaatgctgg
     1021 ctgacggtcg aaactgcggt aagaatcggc ttgcgcgcac gactcgcctt acgggccaat
     1081 aacgcctaag gaaaacctca tggcaaacta ctttgaatcg gaagacctga aaaaatttgg
     1141 caacatcggc cgcgtcaaca aggagctggc agacaagttc tttgattact acaacagcac
     1201 catgaaagcc ggagcgctgt cggagcgcga aaaggcgctg atcgcgctgg ctgtcgcgca
     1261 cgcgatgaaa tgcccctact gcattgatgc ttatacctcg acctgccttg aaaaaggcgc
     1321 agacgaggcg cagatgtccg aggctgtgca tgttgccgcc gcgatgcagg caggtatcgc
     1381 gctggtgcac agcgtgcaga tgatgaacca catcgacaac gcgagcatat gaaactgaac
     1441 tccgcagagc agttgcggct gctcggcgca caggcagaag ctttcgaggc acgggcggct
     1501 gcctctggtt ttgccggtct gaaaccggca gaactgacaa ctttccaaat caatgtaggc
     1561 aggtggtgca atcaggcatg ccggcattgc cacgtcgatg cttcgcctca gcgcaccgag
     1621 atgatggatc gcgagacact ggaaaagtgt ctgcacatca tcacctccgt tccgtcgatc
     1681 acgacagtcg atatcaccgg cggagcgccc gaaggccacc cgctgttccg cgagttcgtc
     1741 acac
//
LOCUS       DAOQJF010000508         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_60195_length_1744_cov_3.950858, whole genome shotgun sequence.
ACCESSION   DAOQJF010000508 DAOQJF010000000
VERSION     DAOQJF010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60195_length_1744_cov_3.950858"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLF85_17170"
     CDS             complement(<1..472)
                     /locus_tag="PLF85_17170"
                     /inference="COORDINATES: protein motif:HMM:NF024412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HNE21453.1"
                     /translation="MYDYQEIYLRIKRNFRYYKGSPIVRLRAMLLLRVLTGINNGRSV
                     ESAAELQGVRRQYVYFWLRRIMAADFDLACLKGISKRPHRSPNATPASVVKRILEIRK
                     FDDSGGHTIALILRRDSGIPIAGSTACAVLKREGVSNVYRYHKHNEHTKRYSAEN"
     gene            601..1461
                     /locus_tag="PLF85_17175"
     CDS             601..1461
                     /locus_tag="PLF85_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE21454.1"
                     /translation="MPRNSDLMKIHHLQTASFCPFGGPLVHDRGLAGGEAHLLCHSLL
                     LETNDGLVLIDTGFGTDDLENPRERLGAAFLTLMKVNFDRKLTAAQQVEALGFKRNDV
                     RHIIPTHLDLDHAGGMPDFPKAKVHIYEDEYLAAMHPLTFNEKQRYRQVHWRHLPEWQ
                     RYTLEGESWFGFPAVKPLLGAGKDEILLIPVTGHTRGHTAVAVRSEGRWLLHCGDAYF
                     HKGQMDLSHEHCPVGLELFQRIMAVDNEARVENLARLRTLKQDHGHEIELFSAHDKDE
                     FERLAARAAV"
     gene            1458..>1744
                     /locus_tag="PLF85_17180"
     CDS             1458..>1744
                     /locus_tag="PLF85_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21455.1"
                     /translation="MSTGRASNFQRAINVFFEKYFANSGFTRFLLGVSDYFWFSRRVF
                     QSALHLRGKHLDTFVRVVINQVRFTGAQALPMLSLIALSIGATTIIQAITF"
BASE COUNT          389 a          476 c          448 g          431 t
ORIGIN      
        1 ggttttctgc actataccgc ttggtgtgct cgttatgctt gtggtagcgg tagacgttgc
       61 ttacaccttc acgcttcaga accgcgcatg ccgtagaacc tgcaatggga atacccgaat
      121 cccgccgcaa gattagtgca attgtatgcc cgccagaatc atcaaacttg cggatttcta
      181 aaatcctctt tactacgctc gccggggtcg cattcggtga gcgatgtggt cgtttcgaaa
      241 tgccctttaa gcaagccaga tcaaagtcgg ctgccattat gcgacgcagc cagaaataga
      301 catactgtcg ccgcaccccc tgcagttcag cggcgctctc cacagagcgt ccattgttga
      361 tgccagtcaa aacccgtaac aacagcatgg cccgcaaccg tactatcggc gaccccttgt
      421 agtaacgaaa attgcgcttt atgcgcagat agatttcttg gtaatcgtac atttgcccgt
      481 ccccctaact aagttttgtg gtaaaaaccg ttaaagggcg ggcgcttatc ttagcaatcc
      541 cactgtaaca tatgtccccg gactgaagag gcgggtaaag gatttgcttg tcgggtatga
      601 atgccacgta attctgacct gatgaaaatt caccacctgc agactgcgag tttctgcccg
      661 ttcggcggtc ctctagtgca cgatcgcggc ctcgccgggg gtgaagctca cctgttatgc
      721 cattcgttac tgcttgagac caatgacgga ttagtactga ttgacaccgg ctttggtacc
      781 gatgatctcg aaaatccacg cgaaaggctc ggcgccgctt ttctcacgct gatgaaagtc
      841 aactttgacc gcaagctcac tgcggcgcag caggttgagg cattggggtt caagcgtaat
      901 gatgttcggc acattattcc gacgcacctc gaccttgacc atgcgggtgg tatgcctgat
      961 tttccgaaag cgaaagtcca catttatgaa gatgaatatc tcgccgcgat gcacccgctg
     1021 accttcaatg aaaaacagcg ctaccgtcag gtgcactggc gccatttgcc cgaatggcag
     1081 cgctatacgc tcgaaggtga gtcgtggttt ggatttccgg cggtgaaacc gcttttagga
     1141 gcaggcaaag acgaaattct gctgatacct gtcacgggtc atacacgcgg ccataccgcc
     1201 gttgccgtgc gcagcgaagg gcgctggctg ttgcattgtg gcgacgccta ttttcacaaa
     1261 ggccagatgg acctctcgca tgagcattgc ccggtcggtc tggaactttt tcagcgcatc
     1321 atggcggtcg ataacgaagc gcgcgtcgaa aaccttgccc gcctacgcac actcaaacaa
     1381 gaccatggcc acgaaatcga actgttcagc gcgcatgata aagatgaatt cgagagattg
     1441 gccgctcgcg cagccgtatg agcaccgggc gcgccagtaa ttttcagcgc gccatcaacg
     1501 ttttttttga gaaatatttt gcgaattcgg gtttcacccg ctttttactc ggggtgagcg
     1561 attatttctg gttttcccgc cgcgtctttc agtctgcatt gcacctgcgc ggtaagcacc
     1621 tcgatacctt tgtacgcgtc gtcatcaatc aggtgcgctt caccggagca caggcgctgc
     1681 cgatgctgtc gctgattgcg ctctcgatcg gtgcaacgac gattattcag gcaattacct
     1741 tttt
//
LOCUS       DAOQJF010000509         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_60321_length_1742_cov_5.110848, whole genome shotgun sequence.
ACCESSION   DAOQJF010000509 DAOQJF010000000
VERSION     DAOQJF010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60321_length_1742_cov_5.110848"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLF85_17185"
     CDS             complement(<1..968)
                     /locus_tag="PLF85_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE21456.1"
                     /translation="MHKITISGVTYAWPGEDPLFQNISFTLGRGIFALAGPNGTGKSS
                     LLRLLAGELQPQSGSIQCSVSPRFIGTAEQEPARRSSGEQQMQQLETTLAQNSGILLL
                     DEPERHLDTTNRRRFIQRLRFFGGTVLLATHDPELLDLAESILHLEVKSLSLYRMDYA
                     DYCSAREAEQAATEYALQRAERSVHKELLDAQRDLERQLRRERIAAERAPLAGIPRVA
                     RGLMKRNAEKTRGKIISRTKDRIIAGKDELARLRGSNLPKAEFAFACTGAQKHHSRVD
                     AFSLNLFNTRGESLWSKPLSLTARAGEKILITGRNGAGKSMLFRALL"
     gene            1172..>1742
                     /locus_tag="PLF85_17190"
     CDS             1172..>1742
                     /locus_tag="PLF85_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNE21457.1"
                     /translation="MQRKAKDKKTTAGAKARAVSIMDTTLRDGEQTYNVAFAPSEKLQ
                     MARLLLEQANVNFIEVASARVSKGELEGVQLITDWAKKSGGRKMLNRIEILGFVDEKR
                     SVNWLVEAGAYTLNMLTKGSLNHLTRQLKKTPAEHFADIARTIDYAKKHDRAVNIYLE
                     DWSNGWLHSREYMFSFIDFLVTQPVARIML"
BASE COUNT          389 a          483 c          490 g          380 t
ORIGIN      
        1 agaagtgcgc gaaagagcat actcttgccg gcaccattgc gcccggtgat gagaatcttt
       61 tcgcctgcac gggccgtcag cgaaagcggc tttgaccaga gactttcccc gcgcgtgttg
      121 aaaaggttca gcgagaatgc atccacccgc gaatggtgct tctgtgcccc cgtgcaggca
      181 aaagcaaatt cggctttagg caggtttgat ccgcgcaatc gtgccagttc atctttgccg
      241 gcaataatgc gatcttttgt ccggctaata atcttaccgc gggttttttc cgcgttgcgt
      301 ttcatgagcc cccgcgcgac gcgcggtata ccagccagtg gagcccgctc tgcagctatt
      361 ctttcgcgcc gcagctggcg ctcaagatct cgctgcgcat cgagtaactc tttgtgcaca
      421 ctgcgttcgg cccgttgcag ggcatattct gtggctgcct gctctgcctc gcgtgcgctg
      481 cagtaatcgg cataatccat ccggtagagc gagagggatt taacctcgag atgcagaatg
      541 ctttctgcaa gatccagcag ttctgggtca tgcgtagcca gaagaacggt gccgccgaaa
      601 aacctgagcc gctggatgaa gcgccggcgg ttggtcgtgt cgaggtgccg ttcaggttca
      661 tccaacagca gaatgccgga attctgcgct agagttgtct ccagctgctg catctgttgt
      721 tcgccgctgc tccgtcttgc aggttcttgc tcagctgtgc cgatgaaccg tgggctcaca
      781 gagcattgaa tgctgccaga ctgtggttgc agctcgcccg ccaataagcg tagcaggctg
      841 ctttttcccg tgccgttggg acctgcaaga gcaaagatgc cgcggcccag ggtaaaagag
      901 atgttctgaa aaagcgggtc ttcgcccggc caggcgtacg taacgcccga aatagtgatt
      961 ttatgcatat taactccctg ttgcgccccc gaaggagcgg ttcgtgcttc gtgtgaaagg
     1021 tgcgaacggg aattagcgca tagacgtagc ggtctgctac gcctctattt taatcatgaa
     1081 gcgtcctggc ggcgaaaaaa gttcagcagg ggctgagtca attcgtgctt caccgcatga
     1141 aaggggaata gacgcgggta acctattccc tatgcagcgc aaagccaaag acaaaaaaac
     1201 gactgccggc gcgaaagcac gcgcagtgag tattatggat acgacgctgc gcgacggcga
     1261 gcagacgtat aatgttgcgt ttgccccgtc agaaaaacta cagatggcgc ggctgcttct
     1321 cgaacaggca aacgtcaact tcatcgaggt ggcctcagcc cgcgtttcca agggggagct
     1381 tgaaggtgtg cagctcatca ccgattgggc aaaaaaatcc ggcggccgca aaatgctcaa
     1441 ccgcatagaa atactcggtt ttgtcgatga aaagcgctcg gtaaactggt tggttgaggc
     1501 cggcgcctat acgctcaata tgctcacaaa aggcagcctg aaccacctga cacggcagct
     1561 caagaaaacc ccggccgagc acttcgccga catcgcgcgc acgattgact atgcgaaaaa
     1621 acacgaccgc gcggtgaaca tctacctcga agactggtcg aatggctggc tgcactcgcg
     1681 cgaatatatg ttttccttca tcgatttcct ggtcacccag cccgtggccc gcatcatgct
     1741 gc
//
LOCUS       DAOQJF010000510         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_60324_length_1742_cov_4.340249, whole genome shotgun sequence.
ACCESSION   DAOQJF010000510 DAOQJF010000000
VERSION     DAOQJF010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60324_length_1742_cov_4.340249"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="serC"
                     /locus_tag="PLF85_17195"
     CDS             complement(<1..964)
                     /gene="serC"
                     /locus_tag="PLF85_17195"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNE21458.1"
                     /translation="MFNHRIHNFSAGPAAIPLPVLKKAQEEFLNFQGTGMSIMEMSHR
                     SKEFEAVLARAIDGVKSVLGVPDDYAILFIQGGASLQFSMVPMNLALPGKKIDLIQTG
                     AWTQKAEKEAKKVGEVNIVATTESENFMRLPRTDELKFSADAAYVHMCSNNTIFGTEY
                     NSLPDTKGVPIVADMSSNIASREIDVAKYGLIFAGAQKNLGPSGVTLVIMKKELAERA
                     DKKLPSMLQYRTYIAENSLYNTPPTFGIYMLALTMEHIKETGGLKATEARNVAKAQTL
                     YDAIDNSGGFYRCPVNKADRSRMNVNYRIYKNDAADEALEKQFVS"
     gene            1075..1413
                     /locus_tag="PLF85_17200"
     CDS             1075..1413
                     /locus_tag="PLF85_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21459.1"
                     /translation="MNMGALSLLLHVELPRKQGAGTCYIVGQQYSEKFPEIVMVELSN
                     GTDTPHFQYRFEVLRDIEAGWNYYVAELKNGKLLPLHKVRVVTIGKEQFLRIDEQGEP
                     RDYLGEIPEI"
     gene            complement(1456..>1742)
                     /locus_tag="PLF85_17205"
     CDS             complement(1456..>1742)
                     /locus_tag="PLF85_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21460.1"
                     /translation="ISILIALYAPGLVAAPDATEPPEYYCHEKKEDRYIFADADQKDE
                     CRDMGGRFGTFSRKERKYFCRLSDSGFYLGKSYSRKKAEEKCAEASGTLQ"
BASE COUNT          352 a          462 c          494 g          434 t
ORIGIN      
        1 cgctgacaaa ttgtttctcg agagcttcat ctgcagcatc gttcttgtaa atgcggtagt
       61 ttacgttcat gcgtgaacga tcagctttgt tgaccggaca gcggtaaaaa ccacccgaat
      121 tgtctatcgc atcatagagt gtctgcgcct tcgcgacgtt gcgggcttct gtcgctttta
      181 gtccgccggt ttctttgata tgctccatag tgagcgcgag catatagatg ccgaatgtcg
      241 gcggcgtgtt gtagagggag ttctcggcga tgtaggtgcg gtattgcagc atgctgggca
      301 atttcttgtc tgcgcgctcg gcaagctctt tcttcatgat gaccagggtc acacctgatg
      361 gtccaaggtt cttctgcgcg ccggcaaaaa tcaaaccgta tttggcgacg tcgatttccc
      421 gcgacgcgat gttcgacgac atatcggcaa cgatcggaac acccttggtg tcgggcagcg
      481 agttgtactc ggtgccgaag atagtgttgt tcgagcacat gtgcacgtac gcagcatcag
      541 cagaaaattt cagctcgtcg gtgcgtggca ggcgcatgaa gttttcgctt tcggtggtgg
      601 cgacgatgtt cacctcgccc accttcttcg cttctttctc agccttctgt gtccaggcgc
      661 cggtctgtat gaggtcgatc ttcttgcccg gcagggcaag gttcatcggc accatgctga
      721 attgcaggct cgctccgccc tggatgaaaa ggattgcgta gtcgtcgggt accccgagta
      781 ccgatttcac gccatcgatg gcgcgcgcga gaaccgcttc aaactccttc gaacggtgcg
      841 acatctccat gatggacatg ccggtgccct ggaagttcag gaattcttcc tgtgctttct
      901 tgagaacagg caggggtatc gctgcaggac cggcgctgaa gttgtggata cggtggttaa
      961 acatgcgtcc aaaaatgggg cagagacgag ccgtaaaggc gagtcttgtt tcgcggtggt
     1021 acacgggcac cccgaacgcg ctttacatca ggccacggcg ggtaactcag tgccatgaat
     1081 atgggagctt tatctctgct attgcacgtg gaactgccgc gcaagcaggg agccgggact
     1141 tgttacatcg ttggccagca gtattccgag aaattcccgg agattgtcat ggtggagctt
     1201 tcgaatggta ccgacacccc gcacttccag taccgtttcg aggtgctgcg agatatcgag
     1261 gccggctgga actactacgt cgccgagctg aagaacggca aactgctgcc gttgcacaaa
     1321 gtcagggtag tgaccatagg caaagaacag ttcttgcgca tcgacgagca gggcgagccc
     1381 cgcgattacc tgggtgaaat acctgagatc taacaggctc cggaacctga cgcgcagccc
     1441 cttaccgctt gcgcattact gtagtgtgcc acttgcctcg gcgcatttct cttctgcctt
     1501 tttgcggctg taagacttgc cgaggtagaa tcctgagtcg ctcaatctgc agaagtattt
     1561 gcgttctttt cggctgaatg tgccaaaacg gccgcccata tctctgcatt cgtctttctg
     1621 gtcggcgtca gcaaagatat accgatcttc ctttttctcg tggcaatagt actccggagg
     1681 ttcagttgcg tcgggcgccg ccaccagccc gggtgcatac agcgctatca gaatgctgat
     1741 ca
//
LOCUS       DAOQJF010000511         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_61010_length_1732_cov_5.276685, whole genome shotgun sequence.
ACCESSION   DAOQJF010000511 DAOQJF010000000
VERSION     DAOQJF010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61010_length_1732_cov_5.276685"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLF85_17210"
     CDS             complement(<1..408)
                     /locus_tag="PLF85_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21461.1"
                     /translation="MSRKLQLLFLCALILLFIGPVSSIAGNELPVSSPFFRPYEGDFS
                     IEMVVGYKAQSSRLTIPALDSARETMSKEASTGIALNYGVTEYFSIGVSGKFMADQDY
                     SLIQSGPAFAGNPGSNSRNRGFYDAGFQLGLRML"
     gene            complement(405..>1732)
                     /locus_tag="PLF85_17215"
     CDS             complement(405..>1732)
                     /locus_tag="PLF85_17215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE21462.1"
                     /translation="EIWKFISSANEADLQAELIRYVSLFQINEVLSSTLEYDEVLRLV
                     LQEMRTLSGAEIASIFLINKKDQVLEFAATTDEKADLLKNIRVPLGKGISGYVAQTGE
                     YVNQSDIQSDTRYYREVDVTRGGETKSYLCVPLKLRGEIKGTVQLMNKAGGHPFSDAD
                     VRLMLNFSSQAAMAIETSLLHREALTKKAFERDVHLAADIQRQSLPNTMPQVPGYPLF
                     GHTEPAQDVGGDYFQFINHVDRTGNNKLDIIVADVAGKGIPASLIVSNFHAALQLLSP
                     LYDTLGELAFQLNNFMRQNLITGTFVTAFIARLDAATGRMHYVSCGHNPPFIFRKADG
                     QYTAELLQQSGTAFGLRYDMEYRVHRLDFKPGETLLIYSDGITEAMDRENVQYETDRM
                     NASVIATLSKQGSANVAEQIVENLRKDVDRFRQGAEVNDDLTVVCLQRE"
BASE COUNT          421 a          465 c          430 g          416 t
ORIGIN      
        1 cagcattctc aggccgagct ggaaacctgc atcgtaaaaa ccccggttgc gcgaatttga
       61 tcccgggttt ccggcaaacg cagggcccga ttggatgaga ctgtaatcct gatcggccat
      121 gaattttccg gatacaccga tcgaaaaata ttctgtgaca ccataattca atgcaatgcc
      181 ggtggaggcc tcttttgaca tcgtttcgcg tgccgaatcg agcgccggaa tcgtcaacct
      241 gctcgactgg gccttataac cgacaaccat ttcgatggaa aaatcaccct cgtaaggtct
      301 gaaaaacgga ctactgacgg gcaattcgtt gccggcaatt gaggacaccg gcccaataaa
      361 caagagaatc aaagcgcaga gaaagaggag ctgaagcttt cgactcattc cctctgcaga
      421 caaacaactg taagatcgtc attaacttct gcaccctggc gaaaacgatc aacatctttg
      481 cgcaggttct ccactatctg ctcggcaaca tttgcgctac cctgctttga cagagtcgca
      541 atcacgcttg cgttcatgcg atcggtttcg tactgcacat tttcccgatc catggcttcg
      601 gtaataccat cggaatagat caaaagggtt tcgccgggct taaaatccaa cctgtgtacc
      661 cggtattcca tatcgtagcg taacccgaag gcagtgccac tctgttgcag caactcagcc
      721 gtatactgcc cgtcagcctt gcgaaagata aaggggggat tatgcccgca gctgacgtaa
      781 tgcattctgc ctgtggcggc gtcgagtcgt gctatgaatg ccgtcacaaa ggtaccagta
      841 atcaggttct gccgcatgaa gttattcagc tggaaggcaa gttcgcccag cgtgtcgtag
      901 agtggtgaaa gcagctgcag cgctgcatga aaattcgaaa cgatgagcga tgcgggaatt
      961 ccctttccgg ctacatccgc aacgatgata tcgagtttat tgttacccgt gcggtcgacg
     1021 tgattaataa actggaagta gtcaccaccg acatcctgcg caggttcggt atgcccaaac
     1081 agaggatagc ccggcacctg cggcatcgta ttcggcagag actgtcgctg tatgtctgct
     1141 gcaagatgca catcacgctc aaacgccttt ttggtaaggg cttcgcggtg caggagcgaa
     1201 gtctcaatcg ccattgccgc ctggctggag aaattcagca tcagcctgac atctgcgtcg
     1261 ctgaacgggt gcccgcccgc tttgttcatg agctgcaccg tgcctttaat ctcgccgcgc
     1321 agtttcagcg gcacgcaaag atacgacttg gtctccccgc cccgtgttac atctacttct
     1381 ctataatagc gcgtatcact ctgtatatcg ctctggttta cgtactcgcc ggtttgcgca
     1441 acatacccgg aaattccctt accgagcggc acacggatgt tcttgagcaa atctgctttt
     1501 tcgtcggttg tagcggcgaa ttccagcacc tggtctttct tattgataag gaagatagag
     1561 gcaatttcgg cgcccgaaag cgtgcgcatt tcctgcaata ccagccgcaa gacttcgtca
     1621 tattcaagtg tcgaggagag cacttcattg atctggaaaa gcgagacgta acggataagc
     1681 tccgcctgca aatcagcttc gttggcgctt gagataaact tccagatttc gt
//
LOCUS       DAOQJF010000512         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_62518_length_1711_cov_3.884058, whole genome shotgun sequence.
ACCESSION   DAOQJF010000512 DAOQJF010000000
VERSION     DAOQJF010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62518_length_1711_cov_3.884058"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..699)
                     /locus_tag="PLF85_17220"
                     /pseudo
     CDS             complement(1..699)
                     /locus_tag="PLF85_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
     gene            complement(866..>1711)
                     /locus_tag="PLF85_17225"
     CDS             complement(866..>1711)
                     /locus_tag="PLF85_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21463.1"
                     /translation="GRAKVIYNDERVASSGRQQMVNRSIVNDVFTVDRQQGAQLMGRL
                     FDNTPADFNYVVGVFGGRGVGERLNDDANMMYAARLQWNTVGDPIEWSQSDHKYTQRW
                     QLNIAAGAATNKSNCTAFETDTNSCRALTGYRTSTAGTAIVSAGQFKIDQAVFEIRSV
                     YKGLYFKHESHVKRVVDQSINVNTWPREAEMWGALVQLGYFPHAVLDFVPSELEVAGR
                     YAWVDPNIAVSGNDQREYAGILNWFANGHNNKISFEVTHHVLGNQAGGSVSEQRYRSQ
                     WEFTF"
BASE COUNT          365 a          485 c          476 g          385 t
ORIGIN      
        1 gacgatttct ttgtcgacaa aactcatttc gatatcgatc tgcgtgaact caggctggcg
       61 gtcgcggcgc aaatcttcgt cgcggaagca gcgcgcgatc tggtaataac gttctgagcc
      121 cgcaaccatc agcagctgct tgaagatctg cggcgattgg ggcagcgcgt agaactcgcc
      181 cgcattgagg cggctcggca cgagaaagtc gcgcgcgcct tcgggtgttg atttattcag
      241 tataggagtt tcaatttccc agaagccttc accgtcgagg taatttctca ccgaacggat
      301 aaactcgtgg cgtacacgca gcgattcctg catatttttg cggcgcaggt caaggaagcg
      361 gtattcgagg cgcagctctt cagaagcctc gtcgtcgtgc tcgtcgagcg gaatcgggga
      421 aggtttcgct ttgctgagga gttcaagccc tgtggcgacg atttcgattt caccggtcgc
      481 cagcttgggg ttaaccgccg tggccgcgcg cttacgcacc ttgcccttca ctgcgataac
      541 gtcttcagcg cgcagtgaat cggcacgttt caaaaggtcg gcgtctacag ccagatcgaa
      601 gacgacctgt accacgccac tgcggtcgcg caggtcgacg aacagaacgc cgccctggtc
      661 gcggtagcgg tatacccagc cgcagacgag tatttctttt tcgctgtcag ccggcctgac
      721 agagttgcat tcgcgacggt cggtgaaatt gacggaagag gcttttacct cagatggaga
      781 cataaattaa aggaaaaacc ggggtttgac accccgcgtc aatgattgtc cggatcaggt
      841 gccgtgcaca tgatctggac agatttcaga aagtgaactc ccactggctg cggtagcgct
      901 gctcagaaac cgagccgcct gcctggttgc ccagcacatg gtgggtgact tcgaaggata
      961 tcttattgtt gtggccattg gcaaaccagt tgagtatgcc ggcatattcg cgctggtcgt
     1021 taccgctgac ggcaatattc ggatcaaccc aggcataacg gcctgccact tcgagctcag
     1081 agggcacgaa atcgagtacc gcgtgcggaa aatagccgag ctgaaccagc gctccccaca
     1141 tctccgcttc gcgcggccag gtattcacgt taatactttg gtcgacgaca cgcttcacgt
     1201 gtgattcatg cttaaaatat agccccttgt agaccgagcg gatttcaaac actgcctggt
     1261 caatcttgaa ctggccagcc gagacaatcg ccgtacctgc cgtgctcgtc ctgtagccag
     1321 tcagcgcccg gcagctattt gtatctgtct caaaggcagt acagttgctc ttattggttg
     1381 cggcaccggc tgcaatattg agctgccagc gctgcgtgta cttatgatcg ctctggctcc
     1441 actcaatcgg gtcaccgacc gtgttccact gcaggcgggc agcatacatc atgttggcat
     1501 cgtcattcag gcgctcaccg acaccacggc cgccaaagac acccaccacg tagttgaagt
     1561 cggccggagt attgtcaaag agccggccca tcagctgtgc gccctgctgg cggtcgaccg
     1621 tgaagacgtc gttcacaatc gagcggttga ccatctgctg acggcctgac gaggctacgc
     1681 gttcatcatt atagatcact tttgcgcgac c
//
LOCUS       DAOQJF010000513         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_63436_length_1699_cov_4.855839, whole genome shotgun sequence.
ACCESSION   DAOQJF010000513 DAOQJF010000000
VERSION     DAOQJF010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63436_length_1699_cov_4.855839"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PLF85_17230"
     CDS             <1..1162
                     /locus_tag="PLF85_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21464.1"
                     /translation="FWPRNLPLDLPARDIRINADGSVSVVPGPFQLPYLADDIAPPDS
                     ELSHFTRNLKNAELFIAEGELQLTRIIYERLLKQVIDEEARRKIRANLEALDNYRKSH
                     DWGNFMPIPPWMRNPWQDIKPPQISVSEMPVEAKSITINIDKGFFDVARAAFEQQKSA
                     AAEAEEAGGESQIGEEEPGTAEAAQGEEAKEEERRESEDRRQGLPDTRGEGAEENAPQ
                     PPLAADGTAAAPEAEPPAADGVMEQPEIPQMAPDGTAQSGAAGAGEEEGDGKEKEEEN
                     KVQEIRGVLELKTPDQEDTPFLTLTYDFTKIPHAYRLAKDNGIFEYAYYKYKPMLVKA
                     HQFIKRKQITRALNYYRVIREQQIPTEFRHMVDRNIKDITEYLQKYLVTRQN"
     gene            1166..>1699
                     /locus_tag="PLF85_17235"
     CDS             1166..>1699
                     /locus_tag="PLF85_17235"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNE21465.1"
                     /translation="MALLIVGVAAVIFAVWHSFSDALAWRIWQAWYSPERKKIPHKIS
                     FVFGSRSRCEFCGAAIPWYGLIPVLGYFLLQGKCQNCSRRLSLRFPAFEFAALVYGAL
                     LGTFLVSPGEFLTALFAYALVWIIIHNDYRSLLIPTEAILALFLVALANLLLVRYPRW
                     FEMQDLSLGLDLAVAFVW"
BASE COUNT          390 a          463 c          489 g          357 t
ORIGIN      
        1 tttctggccg cgaaacctgc cgctcgatct tcccgcgcgg gacatacgca tcaacgccga
       61 tggtagcgta tcggttgtgc cagggccctt ccagttacct tatctcgcag acgatatcgc
      121 tcccccggac agcgaacttt cgcacttcac gcgtaacctg aagaacgctg agctgtttat
      181 tgctgaaggc gagttgcaac tcacgcgcat tatctacgag cgcctgttga agcaggttat
      241 cgatgaagag gcgcggcgca agatacgcgc taacctcgaa gcgcttgata actaccgcaa
      301 gtcgcacgat tggggcaact tcatgccgat accgccgtgg atgcgcaatc cgtggcagga
      361 catcaaaccg ccgcagatca gtgtcagcga aatgccggtc gaggccaagt cgatcactat
      421 caacatcgac aagggttttt ttgacgtggc gcgtgctgcc ttcgagcagc agaaatctgc
      481 cgcagcagaa gcggaagaag ctggcggcga atcgcagatc ggcgaagaag aaccaggtac
      541 tgctgaagct gcacagggcg aagaagcaaa agaagaagag cggcgcgaga gcgaagacag
      601 gcggcagggg ctacccgata cccggggcga aggggcagaa gagaacgcac cacaacctcc
      661 gcttgcagca gacggcaccg ctgctgctcc tgaggcagaa ccacccgctg ccgatggtgt
      721 tatggagcag ccggaaattc ctcaaatggc gccggatggt accgcacaat ccggtgccgc
      781 cggggccggt gaagaagagg gcgacggcaa agagaaagaa gaagaaaaca aggtgcagga
      841 aatcaggggt gtactcgagc tcaagacccc tgaccaggaa gacacgccgt ttttaacgct
      901 gacctatgat tttaccaaga ttccgcacgc ctaccggctg gcgaaagata acggcatatt
      961 cgaatatgcc tactacaagt ataagcccat gctggtcaag gcacaccagt tcatcaagcg
     1021 taagcagatt acgcgcgcgc tcaattatta ccgcgtgatc cgcgaacagc agataccgac
     1081 cgagttcagg cacatggtcg atcggaatat caaagacatc accgaatacc tgcaaaaata
     1141 ccttgtgacg cggcagaact agcgcatggc gttattgatt gtaggggtgg cggcggttat
     1201 ttttgccgtc tggcattcat tttccgatgc gctggcctgg cgtatctggc aggcctggta
     1261 ttcgcccgag cgaaaaaaaa taccgcacaa gatttcgttt gtctttggta gcaggtcgcg
     1321 ctgcgagttc tgcggcgcgg caataccgtg gtatgggctt attcccgtcc tgggctactt
     1381 cttgctccag ggtaaatgcc agaattgcag ccgcaggctc tctctgcgtt ttccggcgtt
     1441 tgaattcgct gcgctggttt atggcgcact gctcgggaca tttcttgttt caccgggcga
     1501 gtttctcacc gcgctcttcg cctatgcgct ggtgtggatc atcattcata acgactatcg
     1561 ctcgttgctg attcccaccg aggcgatact ggctctgttc ctggttgcgc tggctaacct
     1621 actgttggtg cgctacccgc gctggtttga aatgcaggac ctttcgctgg ggctcgacct
     1681 ggccgttgcg tttgtctgg
//
LOCUS       DAOQJF010000514         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_63675_length_1696_cov_3.202316, whole genome shotgun sequence.
ACCESSION   DAOQJF010000514 DAOQJF010000000
VERSION     DAOQJF010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63675_length_1696_cov_3.202316"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLF85_17240"
     CDS             complement(<1..648)
                     /locus_tag="PLF85_17240"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21466.1"
                     /translation="MRTQVYAGEALARYGFPGGHPFSTKRFGAFYDEFLKRELPKRST
                     VKTPTDGDPEILEYFHRHAYVELVKRSSDQGFGLLDSGDTPAFPGCYEAALTVCATAL
                     TAAHAIMQNETDTAFVPIAGLHHARRNTAGGFCIFNDAGIVIEHLMSEHNLTRIAYVD
                     IDAHHGDGVYYEFEQEPRLIFADMHQQHIYPGTGDADETGTGEAVGVKLNIPMAAG"
     gene            complement(844..999)
                     /locus_tag="PLF85_17245"
     CDS             complement(844..999)
                     /locus_tag="PLF85_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21467.1"
                     /translation="MDIYFYIMLAIGFSCGVYLLTRGLAMYVGDAVADRVTATIMKGQ
                     NPTNPKA"
     gene            complement(1220..>1696)
                     /locus_tag="PLF85_17250"
     CDS             complement(1220..>1696)
                     /locus_tag="PLF85_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21468.1"
                     /translation="MKMHITLLLAALFAATAVHPNKEECVQRVQQGYETAKKNAQDKA
                     NRDSKTAAEKRDRDYKAAVQNSEKARRAAEEKYEKSTKKEFDKIAAEIAAAKKQWEAK
                     LKQHNNDTRHPEVKEASEKHRQVTQAANDKRKQVTDAATEQKRADLRADGAKARCS"
BASE COUNT          322 a          459 c          472 g          443 t
ORIGIN      
        1 gccggccgcc atcgggatgt tcagcttcac accgaccgct tcgccggtac cggtctcgtc
       61 ggcgtccccc gtgccggggt agatatgctg ctggtgcatg tcggcgaaga tcaaccgcgg
      121 ctcctgctcg aattcataat acaccccgtc accgtggtgg gcatcgatgt ccacgtatgc
      181 aatgcgcgtg aggttgtgct cagacatcag gtgttcgatc acgataccgg cgtcgttaaa
      241 gatacagaaa ccgcctgcgg tgttgcggcg cgcgtggtgc aggccggcaa ttggcacgaa
      301 cgcggtatcg gtctcgttct gcatgatggc atgcgccgca gtgagcgcag tcgcgcagac
      361 ggtcagtgct gcctcgtaac aaccgggaaa agccggtgtg tcgccactgt cgagcaggcc
      421 gaagccctgg tctgacgaac gtttgaccag ctcgacgtaa gcgtgccgat gaaagtactc
      481 cagaatttcg gggtcgccat cagtgggagt tttcacggta ctgcgcttcg gcaattcgcg
      541 tttcagaaac tcgtcataga acgcgccgaa gcgtttggta ctgaacgggt gaccgccggg
      601 aaaaccgtaa cgggcgagtg cctcgccggc gtagacttgc gtgcgcattt tcaggactct
      661 gtggcttctc tgttcaaacc tattttccgt cccgcaaaat actccagact gagatgatga
      721 aaccagcaag ggttatgatc gtcgtcaaag atgccgtaat atatttgtac tctttgtatg
      781 tttcaagcaa tgcaaacagt gtcccaatga tcagggaacc aagtatgaca taggttttgc
      841 gattcacgcc tttgggtttg tcgggttttg acccttcatg attgtcgccg tgacacggtc
      901 ggcgacagca tcaccgacgt acatcgccaa tccgcgtgtc aacaggtaga cgccgcagct
      961 gaatccaatg gccagcatga tgtagaagta gatatccact gaatgaatct ggttaaattc
     1021 tgtgctaccg tcaataaaaa ttgagattct gaaagtaagg cgccgaagcg tttcgtgctg
     1081 aacagatgcc cgctgtcagt cgtcagagcc gacccgtcgg ccgttcgtgt taacagccga
     1141 cggccgggaa aaccgtaacg ggccagtgct tcgccggcgt aaacttccgt gcgcattata
     1201 ccgattggcg tatcgtgagt tagctacagc gcgcttttgc tccgtcagca cgcagatcag
     1261 ccctcttttg ttctgtcgct gcatcagtca cctgcttacg cttatcgttt gcagcctggg
     1321 tcacctggcg gtgcttttct gacgcctctt tgacttcggg gtgtcgggta tcgttgttgt
     1381 gctgcttgag cttggcttcc cattgcttct tggctgctgc gatttctgcc gctatcttat
     1441 cgaactcttt tttcgtgctt ttttcgtatt tttcttcggc agcgcgccgg gccttttcgc
     1501 tgttctgcac agctgctttg tagtcgcggt cgcgtttttc cgcagcggtc ttgctatcgc
     1561 ggttagcctt atcctgcgca ttcttctttg cagtctcgta accctgttgg acgcgctgca
     1621 cgcattcttc tttgttgggg tgaactgccg tggcagcgaa cagggccgcc aagagcaagg
     1681 taatgtgcat cttcat
//
LOCUS       DAOQJF010000515         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_64202_length_1689_cov_4.122399, whole genome shotgun sequence.
ACCESSION   DAOQJF010000515 DAOQJF010000000
VERSION     DAOQJF010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64202_length_1689_cov_4.122399"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..407
                     /locus_tag="PLF85_17255"
     CDS             141..407
                     /locus_tag="PLF85_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21469.1"
                     /translation="MKTTIQIPDEIWKKLNIEAAERQQKGFSGIITEAVESYLKRKDR
                     ARQLLQKRKRRLIAHNLFGSLSSGEGAEELAALHERRKQWRTHL"
     gene            389..781
                     /locus_tag="PLF85_17260"
     CDS             389..781
                     /locus_tag="PLF85_17260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE21470.1"
                     /translation="MANPFIADTSILINFFRGVPETRYLTSAYEEGQIFIPTIVRYEL
                     LCGVKEQKHREQRKQFLAECQMLDLTPEIADLAGELYSDLRDRGRTIDNEDIIIAATA
                     VVHDLPVATLNVRHFEGINKLRLFTNLN"
     gene            849..1484
                     /locus_tag="PLF85_17265"
     CDS             849..1484
                     /locus_tag="PLF85_17265"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE21471.1"
                     /translation="MSEARRPSPLKRRYLIDLKFQGKFIVTAVLPLLVFVLILAFGFM
                     YAVNSIAREYQFENTAELIQKLSMTLGNDATSGIVFQKVKFYGLLALGGLAIVMILSL
                     SILFVLFSHRIAGPVMRIGRTFEDALHGDLTQRITLREHDELKSTADQLNHMLDALQG
                     RIRRIDQMSRFMHDTLETMLEEAPPEKKEQLLKLDDLTRGVREAISDFKLQ"
     gene            1488..>1689
                     /locus_tag="PLF85_17270"
     CDS             1488..>1689
                     /locus_tag="PLF85_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21472.1"
                     /translation="MPAEILACEPYSALSAVYDGVMHDVPYADWFSFIQHHLQLPGLL
                     IDLFSGTGAIARVAADRGFTAIS"
BASE COUNT          399 a          483 c          434 g          373 t
ORIGIN      
        1 gtaaggcagc ggattttgat tccgccatgc gaaggttcga ccccttccgg cccagaaaag
       61 tgcataaaaa aaccgcctat ggcggttttt ttatgcactt tttaatggcc ttatagccat
      121 atagatggta atatgactgc atgaagacga caatccagat tcctgacgaa atctggaaaa
      181 agctcaacat cgaagctgca gagcgacagc agaaaggctt ctcaggcatt attaccgagg
      241 ccgtcgaaag ttatctgaag cgaaaagaca gggctcgtca gttgctccag aaacggaagc
      301 gcaggctcat cgcgcataac ctgttcggct cgctatcgtc tggcgaaggc gctgaagaac
      361 tcgcagctct gcatgaaagg cgaaagcaat ggcgaaccca tttatagcag atacctcgat
      421 actcatcaat ttctttcgcg gcgtacctga gacacgttac ctgacctctg cgtacgaaga
      481 ggggcaaatc tttataccga ctattgtacg ttacgagctc ctttgcggtg tgaaagagca
      541 gaaacatcgc gagcagcgaa aacagtttct agcggaatgc cagatgctcg atttgacacc
      601 cgaaatagcc gacctggcgg gagagctcta ttcagatctg cgcgaccgcg gccgaacaat
      661 cgataacgag gacattatca ttgctgcaac ggccgtggtg catgatttgc ccgtagccac
      721 gctaaatgtc aggcattttg aaggcatcaa caaattgcga ctctttacca acctgaactg
      781 accggcgcct cgcattgggc gttctataac tctgttgaca tccgcaaccg cccgctgact
      841 gctgcggtat gagcgaagca cgccgcccct cacccctgaa gcgcagatac ctgatcgacc
      901 tcaaattcca gggcaagttt attgtcacag ccgtgctgcc gctgctggta ttcgtgctca
      961 tactggcgtt tggtttcatg tacgcggtca acagcatcgc gcgcgagtac cagtttgaaa
     1021 acacggcgga gctcatacag aaactgtcga tgacgctcgg gaacgatgcc acttcgggca
     1081 tcgtctttca gaaagtgaag ttctacggcc tgctcgcgct cggtggcctc gcgatcgtga
     1141 tgatactttc gctgagcata ctgttcgtcc tgttttcgca ccgcatcgcc ggccccgtga
     1201 tgcgcatcgg tcgcacgttt gaagatgcgc tgcacggcga cctgacgcag cgtatcaccc
     1261 tgcgcgagca cgacgagctc aagtcgactg ccgaccagct gaaccacatg ctcgatgcac
     1321 tgcagggccg tattcggcgt atcgaccaga tgtcgcgctt catgcacgac acgctcgaga
     1381 ccatgctcga agaagcccca cccgagaaaa aagaacagct gctcaaactc gacgacctga
     1441 cgcgcggcgt gcgcgaagcc atctctgact ttaagctgca gtagctcttg cctgcagaaa
     1501 tcctggcctg cgagccctac tcggcgctct ctgctgtcta tgacggcgtc atgcatgacg
     1561 tgccttacgc agactggttt tcctttattc agcaccacct tcagctgcca ggtcttctga
     1621 tcgatctttt cagcggcacg ggcgccatcg ccagagttgc tgccgaccgt ggattcacgg
     1681 ctatcagcc
//
LOCUS       DAOQJF010000516         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_66723_length_1657_cov_3.609863, whole genome shotgun sequence.
ACCESSION   DAOQJF010000516 DAOQJF010000000
VERSION     DAOQJF010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66723_length_1657_cov_3.609863"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLF85_17275"
     CDS             <1..553
                     /locus_tag="PLF85_17275"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE21473.1"
                     /translation="LDGLPDLRPFTFDQKAELVCYANIQTHEVILSRYPYIREKAIYS
                     NVPHLNLQAFPGNEEDGYECLNLGGMQFQPIRMVHIPKAGVLSTGFVVNGKFGYLTDF
                     KEIHPDDEKHLQNLEVLYLGSPIDKPHVSHINHQEALDLFARFQPGRGVIGHLSHQYL
                     HGELIAHWQGKAEPAYDGMWFEI"
     gene            complement(555..1196)
                     /locus_tag="PLF85_17280"
     CDS             complement(555..1196)
                     /locus_tag="PLF85_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21474.1"
                     /translation="MNRLIKIFSPAAAAAIIMSLAACNNYDLLNQLESPGKSTNNTGG
                     FISLYLFSTSTPINGNIKGAFATAREGADAQCLGTRAGITFPNNGCNQVRAVISLSAS
                     DSIGNMPLNYGVPTNRSINASNNFVIAPDWNALRDGTSGNSLAPNVMPAATTWWSFST
                     TGGNFDGTNNCSGGGNGSSFSGVAADSSVTGANWVTGSALICSGSYRLLCICY"
     gene            complement(1297..>1657)
                     /locus_tag="PLF85_17285"
     CDS             complement(1297..>1657)
                     /locus_tag="PLF85_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21475.1"
                     /translation="LNPGMAAEFWASRDNHVLLREMLLTGAIYSSSDSRMKQLFNQVA
                     SPEEIAAAVDFAAKAIADNSPQSLGISVDLLRHPLLSREEIMQREAEGQGKCMSTGEI
                     KESVTAQREGKVYRFQR"
BASE COUNT          367 a          441 c          466 g          383 t
ORIGIN      
        1 tctcgatggc ctgcccgacc tgcgcccgtt tacctttgac cagaaggcgg agctcgtctg
       61 ttatgcgaac atacagacgc acgaggtgat tctcagccgt tacccttaca tacgcgagaa
      121 ggcgatttac agtaatgttc cgcacctgaa cctgcaggcc tttccgggca atgaagagga
      181 tggctatgag tgcctgaacc tgggtggcat gcagtttcag ccgattcgca tggtgcacat
      241 tccgaaggcg ggtgtgctca gcaccgggtt cgtggtaaat ggcaaattcg gctacctgac
      301 cgacttcaaa gaaatacatc ccgacgatga gaagcatctg cagaatcttg aggtactcta
      361 cctcggttca ccgatcgaca agccgcatgt ttcacacatc aaccaccagg aagcgctcga
      421 cctgttcgcg cgctttcagc caggccgcgg agtaattggt cacttgtcgc accagtacct
      481 gcacggcgag ctgatcgcac actggcaggg taaggcagag cctgcgtacg acggtatgtg
      541 gtttgagatt taggtcagta gcagatacag agaagtcggt atgagcctga gcaaatcaag
      601 gcgctgcccg taacccagtt tgcgccggta actgaagaat ctgctgccac gccgctgaaa
      661 ctcgatccat tgcccccgcc cgaacagtta tttgttccgt caaaatttcc acccgttgta
      721 gaaaatgacc accaggtggt tgctgcaggc atgacgttgg gggcaagaga attgccactg
      781 gtgccatcac gcagtgcgtt ccagtcaggc gcaataacaa aattgttgga tgcgttaatg
      841 cttctgttcg tcggtacgcc ataattcagc ggcatgttgc cgatagagtc agacgccgac
      901 agtgaaatta ccgcgcgcac ctggttgcag ccattgttag ggaacgtgat tccagcgcgg
      961 gtacccaggc actgcgcatc ggcaccctcg cgcgcggtgg caaaggcgcc tttaatgttg
     1021 ccattaatgg gtgtactggt cgaaaacagg tagagcgaaa tgaaaccacc tgtgttgttc
     1081 gtactcttgc cgggtgattc cagctggttg agcaggtcgt agttgttgca ggcggcaagt
     1141 gacataatta ttgcggccgc agctgcaggg gaaaatatct tgatcaagcg attcatgata
     1201 cctcttctga cggcaaaagt tggcggggca agacaattta ttttttttta gcgagcagtg
     1261 tgatcacggt ctttgatcaa cgttagtgat aagattctac cgctgaaaac ggtacacctt
     1321 gccttcgcgc tgcgcagtca ccgattcttt gatctctccc gtgctcatgc acttgccctg
     1381 gccttcggct tcgcgctgca tgatttcttc gcgtgacagc agcgggtgac gcagcaggtc
     1441 aaccgagatg ccgagcgatt gcggcgaatt gtcagcaatt gccttcgcgg caaaatcgac
     1501 cgcggctgcg atttcttcag gtgaggccac ctggttgaac agctgcttca tgcggctgtc
     1561 tgagctcgag tagatggccc cggtcagcag catctcgcgc agcagcacgt ggttgtcgcg
     1621 tgacgcccag aactccgccg ccatgccggg gttaagg
//
LOCUS       DAOQJF010000517         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_67431_length_1648_cov_5.042687, whole genome shotgun sequence.
ACCESSION   DAOQJF010000517 DAOQJF010000000
VERSION     DAOQJF010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67431_length_1648_cov_5.042687"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLF85_17290"
     CDS             <1..357
                     /locus_tag="PLF85_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HNE21476.1"
                     /translation="CGSHGLRSAVDETAPRLVLSGHVHEHYGLMKLGQTFFLNPSNFG
                     SVQTMEGEQVGGFFATINLQPVDDGQKFVREVAWKRLAKGKITDICSVKIDKKYRASE
                     EILDEAQFSAMGSFLL"
     gene            362..1042
                     /locus_tag="PLF85_17295"
     CDS             362..1042
                     /locus_tag="PLF85_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21477.1"
                     /translation="MRNPFPENETFRKFLGLKDYFRKFTTPETVQRTRELARAARLIQ
                     STGEKISFDLLGSVNFGMAVESSDVDIVMYLDCGHEEEARYDNTPMLRIYESLFFATI
                     LREISNNPFKLQIVDFINLRRLDRAIREPGFDDDILARFVFYRTICRGVNKLPIRKYE
                     QAISGNKELFRRIEDHLTMALVEFTRSSSHRMSFNKYVSRLQEQDIKIPPSMLEKIQE
                     YLDANDAR"
     gene            complement(1124..>1648)
                     /locus_tag="PLF85_17300"
     CDS             complement(1124..>1648)
                     /locus_tag="PLF85_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21478.1"
                     /translation="DYKQLDGSPTSFDFGTLSRSVQAIFGDAVETHFEPGRFISAPLG
                     ILASRVRHLKNRTGKVIAIIDAAMNDLIRPMLYEAYHPILPVHRRETEVNYDIVGPVC
                     ESTDTFTKGRKIPELHENDWLVIGYAGAYGSCMSSNFNSRPLIPEVLLTTGGELRVIR
                     KPQTLEHMLSPEII"
BASE COUNT          390 a          458 c          440 g          360 t
ORIGIN      
        1 tgcggctcgc acggcctgcg ctccgccgtc gacgagacag ccccgcgcct cgtgctctca
       61 ggccatgtgc acgaacacta cggcctgatg aagctcgggc agacattctt tctcaacccg
      121 tcgaattttg gttctgtaca gacaatggaa ggcgagcagg tcggcggctt ctttgcgacg
      181 attaacctgc aaccggtcga cgacgggcag aagtttgtgc gcgaagttgc ctggaaacgc
      241 ctggcaaaag gcaagatcac tgacatctgc tctgtgaaga tcgacaagaa gtaccgcgcg
      301 agcgaagaga tactcgacga agcgcagttt tcagccatgg ggtcgttctt actctaagcc
      361 aatgcgtaac ccgtttccgg aaaacgagac cttcagaaag ttcctgggcc tgaaagacta
      421 cttccggaaa ttcaccacgc ccgagacggt acagcgtaca cgcgagctcg cacgcgctgc
      481 acgcctgata cagagcaccg gcgaaaaaat ctcgttcgac ctgctgggct ctgtgaattt
      541 cggtatggcg gtcgaaagtt ctgacgtcga tattgtcatg tacctcgact gcggtcacga
      601 agaagaggcg cgctacgaca acacgccgat gctgcgcatc tacgagtcat tatttttcgc
      661 gaccattctg cgtgaaatca gtaacaaccc gtttaagctg cagattgtcg acttcatcaa
      721 cctgcgccgt ctcgaccggg caattcgcga gccgggtttc gacgacgaca ttcttgcccg
      781 ttttgttttc taccgcacca tttgccgtgg cgtcaataaa ctgccaattc gcaagtacga
      841 gcaggcgatc tctggtaaca aagaactctt tcgccgtatc gaggaccacc ttaccatggc
      901 actggtcgaa ttcacgcgct cctcgagcca ccggatgtcg ttcaacaagt acgtttcgcg
      961 cttgcaggaa caggatatca agatccctcc ctcgatgctt gagaagatcc aggagtatct
     1021 ggatgcgaat gacgccaggt aatccgtggt ttgaatcagg gctggtaccg gtactgcaga
     1081 aaagattacg ggccttaacc tgagcggcct tacgccgctt tagttaaatg atttcagggg
     1141 agagcatatg ctcgagggtt tgtggcttgc gtatcacgcg gagctccccc cctgtcgtga
     1201 gcaacacctc gggaatcaac ggccgcgaat tgaaattgga actcatgcac gaaccgtagg
     1261 caccggcgta accgattact aaccagtcgt tttcgtgcag ctcaggaatc ttgcgccctt
     1321 tcgtgaacgt gtcagtcgac tcgcaaaccg gcccaacaat gtcataattc acttctgtct
     1381 cgcgccggtg aaccggcaaa atcgggtggt acgcctcgta cagcatcggg cgaatcaggt
     1441 cgttcatggc tgcatcgatg atggcgatga ctttgcccgt gcggttcttc aggtggcgta
     1501 cgcgtgaagc gagaataccc agcggagcag aaatgaaacg gcccggttca aaatgggttt
     1561 ctaccgcatc gccaaagatt gcctgcacac tgcgcgacag ggtaccaaaa tcaaaagaag
     1621 tcgggctacc atcaagctgc ttgtaatc
//
LOCUS       DAOQJF010000518         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_67526_length_1647_cov_3.179648, whole genome shotgun sequence.
ACCESSION   DAOQJF010000518 DAOQJF010000000
VERSION     DAOQJF010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67526_length_1647_cov_3.179648"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLF85_17305"
     CDS             complement(<1..502)
                     /locus_tag="PLF85_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21479.1"
                     /translation="MRKFLSTESREALWYNSKVGFITLLFAFPPYGIVNHLYYDRPAV
                     NLALPVDHWLPFNHYWILVYAWVYVFLFLPIFIVRGKESFRYVARGYIMLNLVSVAIF
                     LIMPARYPRPEIPTQDEFLWWGTALNYLLDKPVNCFPSLHVGNAFFASMIAFHYRKRV
                     GLIAWFM"
     gene            complement(542..1138)
                     /locus_tag="PLF85_17310"
     CDS             complement(542..1138)
                     /locus_tag="PLF85_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21480.1"
                     /translation="MKKLQQSLALAAVLLSVPATFAQKGVKKEEAPTASITAEPAAAQ
                     PRGRTPQMDATAFGFGAGQYGSLGFLGGHLQIGVADWLRLHGGITGSLPGDQPSGNFA
                     TSNYAFHWLFTGGLMFTGNYYKNIARPYTMLDLTYYLDARSKAGGLNGGIRFGVDLYM
                     TEDWAVYVEAGVNLAFMRETQAPANVGGVVGVGVRTFF"
     gene            1277..>1647
                     /locus_tag="PLF85_17315"
     CDS             1277..>1647
                     /locus_tag="PLF85_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNE21481.1"
                     /translation="MAGTAKLTFNGKEYELPVIQGTEKELAIDISKLRAQSGLITLDD
                     GFVNTGATLSGITFLDGEEGILRYRGYDIAELAEKSTFLEVAYLLIYGNLPRPHEYAT
                     FRESITRHTMIHEDLKRLYDG"
BASE COUNT          399 a          442 c          471 g          335 t
ORIGIN      
        1 ccatgaacca ggctatgagc ccaacgcgtt tgcgatagtg gaaagcgatc atcgaagcga
       61 aaaacgcgtt acccacgtgc aggctgggaa aacagttcac cggcttatcg agcaggtagt
      121 tgagagctgt gccccaccag agaaattcgt cctgcgtcgg tatttcgggc cgcgggtaac
      181 gcgctggcat gatcagaaaa atggcgactg aaaccaggtt gagcataatg taacccctgg
      241 cgacgtagcg aaaactttct ttgccgcgaa cgataaagat tggcagaaac aaaaagacat
      301 agacccacgc atacacaaga atccagtagt ggttaaaggg taaccagtgg tcgacgggta
      361 gagccaggtt aacggcgggc cggtcgtagt agaggtggtt gacgatgccg tacgggggaa
      421 aagcgaacag cagagtgatg aatcccactt tgctgttgta ccagagtgct tccctggatt
      481 ctgtactgag aaattttcgc atgcgtcgcg gacatttgcc cgcgacgcat gctcggtcaa
      541 atcagaaaaa cgtgcgaact ccaacgccga caacaccgcc gacgttggca ggcgcctgtg
      601 tctcgcgcat gaaagcaagg ttgaccccgg cctcaacgta gaccgcccag tcttcagtca
      661 tgtagagatc gacaccgaag cgaatgccgc cgttcaagcc accggccttg ctgcgcgcat
      721 cgagatagta tgtcaggtcg agcatggtgt aggggcgcgc gatgttcttg tagtagttac
      781 ccgtgaacat caggccgcct gtgaacagcc agtggaatgc atagttcgag gtcgcgaagt
      841 tgccgctcgg ctggtcgcct ggcagtgaac cggtaatgcc gccgtgcagg cgcaaccagt
      901 cagcgacgcc gatctgcaga tggccgccca aaaagcccag gctaccgtac tggccggcgc
      961 caaagccgaa ggcagtcgcg tccatctgcg gtgtgcggcc gcgcggctga gccgcagcag
     1021 gttcagctgt gatgctggca gtgggagctt cttctttttt cacgcctttt tgcgcgaagg
     1081 tggcaggcac cgacagcagt actgcggcca gcgccaggga ttgttggagt ttcttcatgg
     1141 tgaacaattg ttcaagtttg tcaggccccg gcaaacgttt tttgcacagg cccgcagtgt
     1201 gctccttgtc caaagttcag cgcagatatc cgaacaattc cttgaaatcc cgggcggatt
     1261 tcatatcttg actgatatgg caggcacagc aaaactcacc tttaacggta aagaatacga
     1321 actacccgta atacagggaa ccgagaagga actggcgatc gacatctcga aactgcgcgc
     1381 tcaatcggga ctgattactc tcgacgatgg tttcgtgaat accggtgcga cgctgagcgg
     1441 catcaccttt ctcgacggcg aagagggcat tctgcgctac cgcggttacg acatcgcaga
     1501 actcgcggaa aagtcgactt ttcttgaggt cgcatacctg ctgatctatg gcaacctgcc
     1561 gcgcccacac gaatatgcaa cattccgcga gtcgatcacg cgccacacga tgatccatga
     1621 agatctcaag cgcctgtacg atggttt
//
LOCUS       DAOQJF010000519         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_67664_length_1645_cov_5.561006, whole genome shotgun sequence.
ACCESSION   DAOQJF010000519 DAOQJF010000000
VERSION     DAOQJF010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67664_length_1645_cov_5.561006"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLF85_17320"
     CDS             <1..1199
                     /locus_tag="PLF85_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNE21482.1"
                     /translation="YTGSGSTEALLQLLRALVAARQSTQNFRAEQKVRLPALVQAPGI
                     QQVLRSPEVTGQAAKLPFLQRADKAGYSASPAVVQVSASLSDSHKRITIANSEGLFIS
                     DERTRVRFAVSVTAEKDGERFASSESPGALRGFEFFDELDIEGLTRSAAERSLRMLSA
                     GYIEGKKMPIILGNGFGGVIFHEACGHPLETEALRKKATPFAGKLGQLVAHPAVTAID
                     DGTIYQSWGSVAIDDEGMPTEKTTLIENGILRNYISDRVGALECNVRRTGSARRESYK
                     YAPVARMRNTYIAAGTSSLDEMLAGIDEGLYAPKLGGGSVNPATGEFNFAVEEGYRIR
                     KGRIAEPVRGATLIGKGHEILPRISMVGSDLEITAGMCGASSGSVPVTVGQPTLKVDE
                     MLVGGR"
     gene            1281..>1645
                     /locus_tag="PLF85_17325"
     CDS             1281..>1645
                     /locus_tag="PLF85_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21483.1"
                     /translation="MNIEEALDYVSDLARKRKLGEFDIVAYDSFSEAVEIFEGKVSNT
                     EISRSAAIGVRVLSDARPGIAFTEKLSPDALEVCLTDAQSHTQLTDKVSFQLPGPMPF
                     DAGNFDRRSQDFARVGFAEL"
BASE COUNT          357 a          462 c          493 g          333 t
ORIGIN      
        1 gctacacagg cagcggttcg accgaagcac ttttgcagct cttgcgggcg ctcgtggcag
       61 cgcgacaatc cacgcagaac ttccgagccg agcagaaagt tcgcctgcct gcgctggtcc
      121 aggcgcccgg tatccagcag gtgctgcgta gccctgaagt gaccggtcag gccgcgaagc
      181 tgcctttcct gcagcgcgcc gacaaggccg gttattcggc ctcgccggca gtcgtgcagg
      241 tcagtgcatc attgagcgac tcgcacaagc gcatcactat cgccaacagt gaaggcctgt
      301 tcatcagcga cgagcggacg cgcgtgcgtt ttgccgtttc agtcaccgcc gaaaaagacg
      361 gcgagcgatt tgccagttct gaatcccctg gagcgctgcg cgggttcgaa ttctttgatg
      421 agctcgatat cgagggcctt acccgctcgg cggcggagcg ctcactcagg atgctctcgg
      481 ccggttatat cgaaggcaag aagatgccga ttatactcgg caatggtttc gggggagtaa
      541 tctttcacga agcctgcggg cacccgctgg aaactgaagc cctgcgtaag aaggcgaccc
      601 cctttgccgg caaattgggc caactggttg cgcatcccgc tgtgacggca atcgacgacg
      661 gcaccatcta ccagagctgg ggctctgtgg ccatcgacga tgaaggcatg ccaacggaaa
      721 aaacaacact gatcgagaat ggtatcttgc gcaattacat ctctgaccgc gtgggagctt
      781 tagagtgcaa cgtgcggcgc accggttctg cgcggcgtga gtcttacaag tacgctcccg
      841 tggcgcgcat gcgcaatacc tacatagctg ccggaacgag ctcactcgac gaaatgctcg
      901 ccggcatcga tgaaggtctt tacgcgccga aactgggtgg cgggtcagtg aacccggcaa
      961 ccggtgaatt taattttgcc gtggaagaag gttaccgtat ccgcaagggg cgaatcgccg
     1021 aacctgtgcg cggggctacc ctcattggca aggggcatga aatcctgccc aggatctcaa
     1081 tggtaggttc tgacctggaa atcaccgccg gtatgtgcgg agcctcatcg ggctccgtgc
     1141 ccgtcaccgt cggccagccg acactgaagg tagacgaaat gctcgtgggg ggtagatgaa
     1201 caggatgttc atgagctttg cgactgccac ggatggcatc tgtgtcgcaa agtagatgac
     1261 catggaggcg acaaaacaca atgaatattg aagaggccct tgattatgtg tcagaccttg
     1321 ccaggaaacg caagctcggc gaatttgaca tcgtcgccta cgatagcttc agcgaggcag
     1381 tagagatttt tgaaggtaag gttagcaata cagaaatctc acgctcggca gcaatcggtg
     1441 tgcgtgtact gagcgatgcc cggccgggca tcgctttcac ggaaaaactc tctcctgatg
     1501 cgcttgaggt ctgccttacc gacgcccagt cgcacacgca gctgacggac aaggtttctt
     1561 ttcagctgcc agggcccatg ccctttgacg ccggcaattt tgaccggcgt tcacaggact
     1621 ttgcgcgagt aggcttcgct gagct
//
LOCUS       DAOQJF010000520         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_68998_length_1628_cov_3.748887, whole genome shotgun sequence.
ACCESSION   DAOQJF010000520 DAOQJF010000000
VERSION     DAOQJF010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68998_length_1628_cov_3.748887"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLF85_17330"
     CDS             complement(<1..196)
                     /locus_tag="PLF85_17330"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE21484.1"
                     /translation="MAFSAEVQRLVTELSLVEHPEGGWYRETYRSPSNTVTPLGERNL
                     ATLIYYLLPAGVESRIHKVSW"
     gene            complement(199..759)
                     /locus_tag="PLF85_17335"
     CDS             complement(199..759)
                     /locus_tag="PLF85_17335"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNE21485.1"
                     /translation="MRTPDFPVDKEFTDRWSPRGFDPSVQLSDTELKTLFEAARWAPS
                     CANEQSWQFYHAKDDASRALFLSFLNEANQYWAKNASHIIIVCARKTFAASGKPNAHS
                     WYDAGAAWMSLALQARKMGYHAHCMAGIQHAVIAEKLKIDTAAVDIVTAIAVGKQGDA
                     SHLNERHKTQETPNARKPQNEFVFAV"
     gene            complement(790..>1628)
                     /locus_tag="PLF85_17340"
     CDS             complement(790..>1628)
                     /locus_tag="PLF85_17340"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNE21486.1"
                     /translation="DPYKGAQHTVAEAVRNVAATGAEPIGLTNCLNFANPYIPENYWF
                     FAQAVTGMSDACREFQVPIVSGNVSFYNESEDGPVLPTPTLGVVGLLEKASDAVPAAP
                     ADLSSNLNLYLCGKFAPSQQASQYHYAEGKPLGGPLPTLDLKAELAGARKMASLSKAG
                     VLSAAIDLSAGGLVRAACRMAFAEFESGGKMPSLSLDTAVLGKGLADTLLLGETAHCY
                     LVAVSDANAAALEKEKSAEFPVVKLGAIKPDGGKLTLGNTELQLNELYSAWAGGLKEF
                     FR"
BASE COUNT          344 a          460 c          467 g          357 t
ORIGIN      
        1 cccacgacac tttgtggata cgcgactcta cgccggcggg cagcaggtaa tagatgagtg
       61 tcgcgagatt ccgctcaccc aggggtgtta cagtatttga cggggagcga taggtttcgc
      121 ggtaccaacc gccctccggg tgctccacaa gactcagctc tgtgacgagt ctttgaactt
      181 cagcagagaa tgccatattc agacggcgaa gacaaactca ttctgcggtt tgcgcgcatt
      241 cggagtttcc tgcgttttat ggcgttcatt cagatgcgat gcgtcgccct gcttgccgac
      301 ggcgattgcc gtgacgatat caaccgctgc tgtatcgatt ttgagttttt ctgcaatgac
      361 tgcatgctgt atacctgcca tgcagtgcgc gtggtaaccc atcttgcgcg cctgcagtgc
      421 aagcgacatc cacgcggcgc cggcatcata ccacgaatgc gcattgggct ttccgctcgc
      481 ggcaaaggtc ttgcgcgcgc agacgatgat gatgtggctc gcatttttcg cccagtactg
      541 gtttgcctcg ttgagaaaac tgagaaagag cgcgcgcgag gcatcgtctt tcgcatggta
      601 aaattgccac gactgctcat tggcgcatga gggcgcccag cgcgcggctt cgaacagtgt
      661 tttgagctca gtatcgctca gctgaacact cggatcaaaa ccgcgtggag accagcggtc
      721 ggtgaattct ttgtctacgg gaaaatcggg ggtacgcatg tattgaatac aaagtactca
      781 atacatgctc tatcggaaaa attctttgag gccaccggcc cacgccgaat acaattcatt
      841 cagctgcaac tctgtattgc cgagcgtcag tttgcccccg tcgggtttta tcgcaccgag
      901 cttcaccacc gggaattcgg cggatttttc tttttcgagc gcagctgcgt ttgcgtcgct
      961 gacggcaacg aggtagcaat gcgcagtttc acccaaaagc agcgtgtcgg cgaggccttt
     1021 gcccagcacc gctgtgtcga gtgaaagtga aggcatcttg ccgcccgatt caaactcagc
     1081 aaacgccata cggcaggccg cgcgcaccag gccgccggca gaaagatcga tagctgcaga
     1141 aagcacacca gccttggaga gcgacgccat tttccgtgcg ccggcaagtt cagccttgag
     1201 atcgagagtg ggcagcggtc cgcctaaggg tttgccttct gcatagtggt actggctcgc
     1261 ctgctgagac ggggcaaatt tgccgcagag atacaggttc aggttcgaag ataaatccgc
     1321 aggcgccgcg ggcactgcat cgctggcttt ttcgagcaaa cccaccacgc cgagcgtggg
     1381 ggtcggcagc accgggccat cttcagactc gttgtaaaac gacacgttgc cgctcacaat
     1441 cggcacctgg aattcgcggc acgcatcgga cataccagtc accgcctgcg cgaagaacca
     1501 gtagttctcg ggaatgtacg ggttcgcaaa gttgaggcag ttggtaagcc caatcggctc
     1561 ggcgcccgtc gctgcgacgt tgcgcacggc ttctgccacg gtgtgctgcg cacccttgta
     1621 aggatcaa
//
LOCUS       DAOQJF010000521         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_69073_length_1627_cov_4.054707, whole genome shotgun sequence.
ACCESSION   DAOQJF010000521 DAOQJF010000000
VERSION     DAOQJF010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69073_length_1627_cov_4.054707"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1627
                     /locus_tag="PLF85_17345"
     CDS             <1..>1627
                     /locus_tag="PLF85_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21487.1"
                     /translation="VAVLPFENRTPNPNIDWISALFVDSYRTALQKRYRFDSPEDLAV
                     SKSLELLQDYRASGKARFQIFAAMTGADIVIGGYFAPAIGQKLTIQSQIFFTRSNRFE
                     ELERLQTPVDSAGLFGAVERSAAESVATIARRSGGDNSSDRQLLLVSYLPRMSFYLPV
                     ETRSGQEKKVLQKLIETQLAPNGTHELAVLAGDALPASLTERQALLVREGVAYAVVSE
                     WVNGRLVLHLHSPLKTEAVASFTGSTGGTREKSAGHALEQLSAFMRGWKFRPRAQVSG
                     LKGKNLQIELSGSKKLETSTDGELQFEQELPLGTDYVITLGKEPHSPAQRCFIVNATG
                     RVTITGTNNVVVVCITQRFTIEGLVEGLSGGEVVLQVNDTERVTLKENATFRIPETFE
                     DYEPLRLIIHARPQEPPQQCDFIDAPMRVMGKATRLHLYCMPLTQHWLTFSVNYPMLQ
                     GNSARSDYLVPNASFPLNALSGRFGVTAGYWAKYYLRYKIMVGGEATYSYYQGKTDLY
                     TASGLLVETGHSLSYHGVGLNAMAGYPIKLPGSS"
BASE COUNT          364 a          468 c          447 g          348 t
ORIGIN      
        1 gcgtcgccgt tctgccgttc gaaaaccgaa cgcctaaccc caatatcgac tggatttcag
       61 ccctgtttgt ggacagctac cgaaccgcgc tgcagaagcg ctatcgtttt gattcacccg
      121 aagaccttgc ggtctccaaa tcgcttgaac ttctgcagga ttaccgggcc agcggcaagg
      181 cacgcttcca gattttcgcc gcgatgaccg gtgcagatat tgtgattggg ggctattttg
      241 ctccagccat tggccagaaa ctcacgattc agtcgcagat ctttttcacc cgcagcaacc
      301 gcttcgaaga actcgaacgc ctgcagacgc cggtcgacag cgccgggctg ttcggtgccg
      361 ttgaacgctc agccgccgaa tcggttgcta ccattgcccg gcgcagcggc ggcgacaact
      421 catctgatcg gcagttatta ttggtttctt acctgccgcg catgtcgttc tatctgccgg
      481 tcgagaccag gtctggacag gaaaagaagg tcctgcaaaa actcatcgaa acccaactgg
      541 ctccaaacgg tacgcatgaa ctagcggtgc tcgcaggcga tgcactgccc gcaagcctca
      601 ccgaacgcca ggcgcttttg gtgcgagaag gtgtcgccta cgccgttgtt tcagaatggg
      661 tgaacggcag gctggtgctg cacctccatt cacccctgaa aaccgaggca gttgcttcgt
      721 tcactggcag caccgggggt acgcgcgaaa aatcagctgg ccatgccctg gaacagcttt
      781 ccgcattcat gcgcggctgg aaatttcgac cgcgtgcaca ggtttcggga ctcaagggta
      841 aaaacctgca aatcgaactc agtggcagca aaaagcttga gacctcgact gacggtgagc
      901 tgcagtttga gcaggagctc ccccttggaa cagactacgt cataacgctg ggaaaagaac
      961 cgcattcgcc tgcacaacgc tgcttcatcg tgaacgccac agggcgtgtg actatcaccg
     1021 gcactaacaa tgtggttgtg gtgtgcatca cgcaacgctt cactatcgaa ggcttagttg
     1081 aagggctgag cggcggcgag gtggtgctgc aggtaaatga taccgagcgg gtgaccctga
     1141 aagaaaacgc caccttccga attcccgaaa cctttgaaga ttacgagccg ttgaggctga
     1201 tcattcatgc gcgccctcaa gagccgccgc agcaatgtga tttcatcgat gccccgatgc
     1261 gagttatggg caaggccaca aggctgcatc tctactgcat gccgctgact cagcactggc
     1321 tgacgttttc ggtgaattat ccgatgctgc agggtaattc cgcgcgcagc gattacctcg
     1381 tgccgaatgc cagctttccg ctgaacgcac tttcgggccg gtttggggtg accgcaggtt
     1441 attgggcaaa atactacctg cgctacaaga taatggtcgg cggcgaagcg acctattcct
     1501 attatcaggg caagacagat ctctacacgg catcggggct cttggtcgaa accgggcatt
     1561 cgctctcata ccacggtgtc ggactcaacg cgatggcagg ttatccaatc aaactgcccg
     1621 gctcttc
//
LOCUS       DAOQJF010000522         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_70034_length_1616_cov_3.791160, whole genome shotgun sequence.
ACCESSION   DAOQJF010000522 DAOQJF010000000
VERSION     DAOQJF010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70034_length_1616_cov_3.791160"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1040)
                     /locus_tag="PLF85_17350"
     CDS             complement(171..1040)
                     /locus_tag="PLF85_17350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE21488.1"
                     /translation="MSTENVIKMNMPLVRRPEFGLEQNFSKYYADGNPFLSHLLNSLH
                     VVFPEGEKLFIRSVKFFQDQIEDTQLKARVKGFIGQETQHMAQHEKIWDTLESQGVNV
                     REYDAWYSKNAYEVVEGTIRKIFGEETYKKLALAVTCGLEHYTATLAEVAIDPEINAM
                     EGFDENMKALLMWHAAEEIEHKAVAFDVMQLVDGSPITKNAGMLLATWALLYYGAIGT
                     VKFVLEDKEIKFTDLPGMLAQKAPKLGKVVAAMVPKLLQYFRPDFHPDQMNNDYLATE
                     YFEANKKYFQKAG"
     gene            complement(1221..>1616)
                     /locus_tag="PLF85_17355"
     CDS             complement(1221..>1616)
                     /locus_tag="PLF85_17355"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE21489.1"
                     /translation="LTIASAGHPPLIIYHARTQSCTRYKPRGMPLGVKDSSPEYTLEY
                     AAVEAGDIVLLYTDGLIEQGVFLGQEFGLDRIERILCDLAKQPAHEISESLLAELEKH
                     AGRLRTQGFDDDIAFVIMKITGDEKKLPR"
BASE COUNT          334 a          423 c          414 g          445 t
ORIGIN      
        1 gaggagcagc gaaggggttg ccactcttga gcattgcctc gcgcacctgc tgcggtgtct
       61 gttgcggcat cggagtctgc cgcttcagta tttttagcag cttcgccgct cgtttgtcta
      121 actgcatatt gtggtcagtt gtcgggggct ctgcccccga caactaaact ttatcccgct
      181 ttctggaagt acttcttgtt tgcttcgaag tattccgttg cgaggtaatc gttgttcatc
      241 tggtcaggat gaaagtctgg cctgaagtac tgcagcagtt taggaaccat tgctgcgaca
      301 actttaccca gtttcggtgc tttttgtgct agcataccgg gcagatcggt gaacttgatc
      361 tctttgtctt cgagtacaaa tttgaccgtg ccaattgcgc cgtagtagag cagcgcccag
      421 gtggcaagca gcataccggc gtttttggtg atggggctgc cgtcgaccag ctgcatcacg
      481 tcgaaggcaa ccgccttgtg ttcaatctct tctgcggcat gccacatgag aagagccttc
      541 atgttctcgt cgaaaccctc catcgcgttg atttcggggt caatcgccac ttcggcgaga
      601 gtcgctgtat agtgctcaag cccgcaggtc accgcgagcg ccagcttctt ataagtctct
      661 tcgccaaaaa ttttgcgtat tgtaccttcg actacttcat aggcattctt cgaataccac
      721 gcatcatact cgcgtacatt gacgccctgg ctctcaaggg tatcccagat cttttcgtgc
      781 tgtgccatat gctgggtttc ctggccgata aagcccttca cgcgggcttt cagttgcgta
      841 tcttcgattt gatcctggaa gaattttact gagcgaatga acagtttttc tccctcagga
      901 aagacaacgt gcaggctgtt caacaggtgg ctgaggaatg gatttccgtc agcatagtac
      961 ttgctgaaat tctgttcgag gccaaactcg ggccgacgaa ccagcggcat gttcatcttg
     1021 atcacattct ctgtactcat ttttttctcc ttggtacacg cgacccgtct ttgggtctcc
     1081 cgttagggag gtacctggtc acagccggca cggatgccat tctggctgcg ataacctgca
     1141 gtgcagcgca ctacacggtt gtgtcaacag ggcaaaaaag cgaacatttc gtcaactttt
     1201 ttatgaacaa gtgttcagtc ttatctgggg agtttcttct catcacctgt aattttcatg
     1261 ataacaaaag cgatatcgtc atcaaagcct tgtgttcgca gcctgcccgc atgtttctcg
     1321 agttctgcca gcaaagattc cgatatctcg tgcgcgggct gttttgcaag gtcgcacaga
     1381 atgcgctcga tacggtcgag gccaaattcc tgtcccagaa atacgccctg ttcgatcaag
     1441 ccgtcggtat agagcaggac aatgtcaccc gcttcgaccg ccgcatattc gagcgtgtac
     1501 tcgggtgaag agtcttttac ccccagtggc atgccgcgcg gcttgtaacg ggtacacgat
     1561 tgtgtgcgag cgtgataaat aatcagcggc gggtgcccgg cagatgcgat agtcag
//
LOCUS       DAOQJF010000523         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_73135_length_1580_cov_6.015082, whole genome shotgun sequence.
ACCESSION   DAOQJF010000523 DAOQJF010000000
VERSION     DAOQJF010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73135_length_1580_cov_6.015082"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..445)
                     /locus_tag="PLF85_17360"
     CDS             complement(17..445)
                     /locus_tag="PLF85_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HNE21490.1"
                     /translation="MKKLPLVIALAIALAAGCKKKEITGKVWFSEPSDKAEVNSPVKF
                     VMQVEGMVVKPAGTMEEGTGHFHILINKEAIAEGQPIINDEVHKHYGKGQTEDTIELK
                     PGDYRITLQFADGAHVSYGSKWSQTISLKVKAPAGAAEEE"
     gene            complement(534..1046)
                     /locus_tag="PLF85_17365"
     CDS             complement(534..1046)
                     /locus_tag="PLF85_17365"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21491.1"
                     /translation="MNVTVLNRGAERLRHIDEVYRGTVTTLPLTSANTERTLAQADMV
                     IGALLVPGGRTPVLIRRADLKKMKPGAVILDVSVDQGGCAETTVPRTHDDPVYTIDGV
                     QHYTVANMPGAYPRTSTFALTNATLPWVLRLAALGADKAVAEFTPLRKALNLQAGKVM
                     NAAVAAAQDL"
     gene            1233..>1580
                     /locus_tag="PLF85_17370"
     CDS             1233..>1580
                     /locus_tag="PLF85_17370"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNE21492.1"
                     /translation="MLINCVVYQEGKKLCDIPVAEISEYLQRPGCFVWVALRDADEVE
                     LNLMQEEFNLHELAVEDARHGHQRPKVEEYGDSVFVVMHLLEKTGTEFAVGEVHIFAG
                     PNYVLSVRNRSSIS"
BASE COUNT          323 a          423 c          453 g          381 t
ORIGIN      
        1 ggtggcaata ggtagattat tcttcttcgg ctgcgcccgc gggggctttc actttgagcg
       61 agattgtctg cgaccatttt gaaccgtaag agacatgcgc cccatcggcg aattgcagtg
      121 tgatacggta atcgccgggt ttgagctcaa tcgtatcttc ggtctggcct ttgccgtagt
      181 gcttgtgtac ttcgtcgttg ataatcggct ggccttcggc aattgcctct ttattgatca
      241 atatatgaaa gtggccggta ccttcttcca tggtgccagc aggtttcaca accatgccct
      301 caacctgcat aacaaacttt accggtgagt tcacctcagc tttgtcagac ggttcggaga
      361 accagacctt gccggtgatt tctttctttt tgcagccagc tgcaagagcg atagccaatg
      421 caataacaag ggggagcttc ttcatatttc attccatgcc gctgaaatca gtatgagcgt
      481 aaagaagttc tatcaggcta gccggtgcgg gagtttttcc ggaagctgct ggtttataag
      541 tcctgcgctg cggcgactgc cgcgttcatt accttgcctg cctgcaggtt gagcgccttg
      601 cgtaacgggg taaactccgc cacagccttg tctgcaccca gggcagcaag gcgcagcacc
      661 catggcaagg tcgcgttggt gagcgcaaat gtcgatgtac gcgggtaggc gcccggcatg
      721 ttcgccaccg tgtagtgttg tacaccgtcg atggtataga ccggatcgtc atgtgtacgg
      781 ggtaccgtag tttcggcgca gccaccctgg tcgaccgaga catcgaggat gaccgcgccc
      841 ggtttcattt ttttcagatc agcgcggcga atcagtaccg gcgtgcgccc acccggaacc
      901 agcagcgccc cgatcaccat gtcggcctgc gccagtgtgc gttcggtgtt ggccgaagtg
      961 agcggcagag ttgtcactgt gccgcgatag acttcatcga tgtgccgcag cctctcggcg
     1021 ccacggttga gtaccgtgac gttcatgcca atgccatgct ccccgcgatt tcgctcatcg
     1081 gtgtgagcag gggcagcgat ccatcgaact cgagcttctt gatttctttg ggtaccccga
     1141 tgcgcataag tacatgcgcc ttcgggcagc acgtctgtaa agagcttcgc gtccgccatt
     1201 cagtgggccc tgaaattggt cgaaaataaa aagtgctcat aaactgcgtc gtctaccagg
     1261 agggcaaaaa actctgcgac ataccggtgg ctgagatcag cgaatacctg caacggccgg
     1321 gttgttttgt ctgggtggcg ctgcgcgacg ccgacgaggt ggagcttaac cttatgcagg
     1381 aggaatttaa cctgcatgag ctggcagtcg aagatgcccg ccacggccac cagcggccaa
     1441 aggtagagga atacggcgat tcagtttttg tcgtgatgca cctgctcgaa aagaccggta
     1501 cggaatttgc ggtcggtgag gtgcatatct tcgccgggcc aaactatgtc ctctcggtac
     1561 gcaatcgcag cagtataagt
//
LOCUS       DAOQJF010000524         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_73201_length_1580_cov_2.477377, whole genome shotgun sequence.
ACCESSION   DAOQJF010000524 DAOQJF010000000
VERSION     DAOQJF010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73201_length_1580_cov_2.477377"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLF85_17375"
     CDS             complement(<1..501)
                     /locus_tag="PLF85_17375"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE21493.1"
                     /translation="MKRIILSASVLFLSVTAVLFALRCAISRPAIFPAPQYRLEPQLP
                     GGGIVKAASTGGREAYGYYAPGGKRLIVFFHGNGEVMGSMQELAAIMQREGFSVPMAE
                     YPGYGYASMYKATEENLYDDAAALLKHMREKYGHSEADTLLWGFSLGTGVAVETGKRQ
                     LGERLLL"
     gene            complement(683..1129)
                     /locus_tag="PLF85_17380"
     CDS             complement(683..1129)
                     /locus_tag="PLF85_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21494.1"
                     /translation="MGRLRRRVLVLPHTLDEHGHVSAYNGEIGDQYGNDAKFQAAFVA
                     RTHHDRPADQIPDQNSDEADFITGKPQNRVIDQRADDTGAYSEHNTFQKLTDTRWLLK
                     MLAQVVGTRGAGALHIYREIRTRKRIMTDTTFGGGFSAFELGFLEL"
     gene            1163..1474
                     /locus_tag="PLF85_17385"
     CDS             1163..1474
                     /locus_tag="PLF85_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21495.1"
                     /translation="MPITQGAKPLSPQQRPKPEAKLSAPTHKPNAAEPAPTLGEALFG
                     LLMFGGIILALPFLAGIENIIGLMIIAFALFEAARRTAKPKLEISGPFTISEPGANAA
                     V"
BASE COUNT          380 a          465 c          397 g          338 t
ORIGIN      
        1 cagaagcagg cgctcgccga gctggcgttt gccggtctca acggcgaccc ctgtgccgag
       61 tgaaaatccc cagagcagcg tatcggcttc gctatgaccg tacttctcgc gcatgtgctt
      121 gagcagcgcc gccgcatcgt cgtacagatt ctcttcggtg gccttgtaca tcgaggcata
      181 accgtaaccg gggtattccg ccatgggcac actaaaaccc tcgcgctgca tgatcgcggc
      241 taattcttgc atcgacccca tcacctcgcc gttaccgtga aagaagacaa tgagcctttt
      301 gccgcccgga gcataataac cgtatgcctc acggccaccg gtcgacgcag ctttgacgat
      361 gcccccgcct ggcaactgcg gttcgaggcg atactgcggc gcgggaaaaa tcgcgggtcg
      421 ggagatagcg caacgcagag cgaatagcac ggccgtaacg ctgagaaaca acacagacgc
      481 ggaaagaatt attcgtttca tcgctgtgat ataatacacg gcagcgcgcg gagcgtcaat
      541 cgttgctgag cctgtctcac ccaacagatc aaaaaatcaa aaaacgacac ctcatctcaa
      601 taaaagaaca ctttaaaatc gcttgtctat accgcgagcg ccccaaccct gtacgatgag
      661 cgaaacagag accgatcaaa agttacagtt cgagaaagcc gagttcgaag gcactaaaac
      721 caccgccaaa tgtagtatct gtcataatcc gctttcgggt tcttatttca cggtaaatat
      781 gcagagcacc tgcccctcgt gtgccgacaa cctgcgccag cattttcaaa agccatcgag
      841 tgtcggtgag tttttgaaaa gtattgtgtt cggaataggc gccggtatca tcggctcttt
      901 gatctattac gcgattctgc ggtttaccgg ttatgaaatc ggcctcgtcg ctattctggt
      961 cgggtatctg gtcggcaggg cggtcatgat gggttctggc aacaaaggcg gcctgaaatt
     1021 tcgcatcatt gccgtactga tcacctatct ctccattgta agcacttacg tgcccatgct
     1081 catcgagggt atgcggcagc accaaaacgc ggcggcgcag ccggcccatg acgccgtgac
     1141 tccacgcagc gcggcggctc ccgtgccaat cacgcaagga gcaaaacccc tctctcccca
     1201 gcagagacct aaacccgagg caaagttgtc tgcacctacc cataagccca atgccgctga
     1261 acctgcgcca acactgggtg aagcgctgtt cggcctgctt atgttcggtg gtattatact
     1321 cgcgctgccc tttcttgccg gaatagaaaa tatcattggc ctcatgatca ttgcttttgc
     1381 tttgttcgag gcggcacggc gcacagcgaa gcccaaactc gaaatcagcg gcccgttcac
     1441 aatttcagaa cccggtgcga atgcagcagt gtaaagattg caacagcgaa ttacccgacc
     1501 acgccatcgt ttgccccgtt tgtgccgcac tggtgtaccg tgcagaactt caagaatcca
     1561 tagccaaagc cgctgccttc
//
LOCUS       DAOQJF010000525         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_73311_length_1578_cov_5.523309, whole genome shotgun sequence.
ACCESSION   DAOQJF010000525 DAOQJF010000000
VERSION     DAOQJF010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73311_length_1578_cov_5.523309"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLF85_17390"
     CDS             complement(<1..718)
                     /locus_tag="PLF85_17390"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE21496.1"
                     /translation="MTNLNEIGILGGGQLARMTIEAAMQLGVRCHVLDKAGDVPAAAF
                     TYRFTEGNPQDFDTVMRFAADLPAITIDSEHVSAQALTALEAEGRIVAPQGRVLEIIQ
                     NKCRQKEFIERCGFPTAKFQVASGRAELAKLSLDFPVVQKTATSGYDGYGVKVLASAA
                     DLEQAFDAESVIEEKIAIAKEIAVIVASDRTGNRVAYDAVEMVFDKQRNILKYQLCPA
                     EISAEVATRATELALSAAKSL"
     gene            complement(715..>1578)
                     /locus_tag="PLF85_17395"
     CDS             complement(715..>1578)
                     /locus_tag="PLF85_17395"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNE21497.1"
                     /translation="KLQEKIKDCEAIVFDLPDSGVRPYTYRTVLNRIMRAMHANASDQ
                     LLYLIDVPNPASHLGPQGPVAQKSNFSYVGEEEIPFMPGFTQAELARKYIADRKLKIR
                     IYIQKMARYNPKQPHSQRGLPFYPPSPNLPHLRSNQCYWISVYFEATSIEEGRITKDP
                     FCQIGHPGFENKELPVMGGVKFQPYPFFAAGGKHKGKLLPGYKLQFSETAVNPTRAAY
                     EFFLWQVKNGKDGFMKKWLAEKQGLDAQTGTTSYRASLIKGLSYEAWLAKEKKHIAEF
                     TAAMQKYRLYK"
BASE COUNT          305 a          440 c          432 g          401 t
ORIGIN      
        1 cgagagattt ggccgccgaa agcgcaagct ccgttgcccg cgtcgccacc tcggcgctga
       61 tttcagcggg gcagagttgg tacttgagta tgttacgctg cttgtcgaac accatttcga
      121 cggcatcgta agcgacacgg ttaccggttc ggtctgaagc aacgatgact gcgatttctt
      181 tagcaatagc gatcttttct tcgatcaccg attcggcatc gaaagcctgc tccagatctg
      241 cggcgcttgc cagcactttc actccatagc cgtcgtatcc cgaggttgcg gtcttttgca
      301 cgacaggaaa atcgagcgat aattttgcga gctcagcgcg accgctggcc acctggaact
      361 tcgccgtggg aaatccgcag cgttcgatga actctttctg ccggcacttg ttctggataa
      421 tttcgagcac ccgtccctgc ggggcgacta tgcggccctc cgcttcgagt gctgttagcg
      481 cctgcgcgct gacgtgctcc gagtcgatgg ttatagccgg caggtcggcc gcaaagcgca
      541 tcaccgtatc gaaatcctgc ggattgcctt cggtgaagcg gtaggtgaaa gctgctgccg
      601 gtacatcgcc tgccttatcg agcacatggc agcgcacgcc cagctgcatc gccgcttcga
      661 tggtcatgcg cgccaactgc ccgccaccca ggattccgat ctcattcaga tttgtcattt
      721 gtataacctg tacttctgca ttgccgccgt gaattcggcg atatgttttt tttccttagc
      781 gagccaggct tcatagctta ggcccttgat cagcgaggcg cggtaactag tcgtgccggt
      841 ttgcgcatcg agcccctgct tttctgcaag ccactttttc atgaagccat ctttgccatt
      901 cttcacttgc cagaggaaaa actcgtatgc tgcgcgcgtc gggttcaccg ccgtctcgct
      961 gaactgcagc ttgtagccgg gtaagagttt tcctttgtgt ttgccgccgg ccgcgaagaa
     1021 agggtaaggt tgaaatttca ctccccccat caccggcagc tctttattct caaaaccggg
     1081 atgcccgatc tggcagaacg ggtctttcgt gatgcgtcct tcttcgatcg acgtcgcttc
     1141 aaaatatact gaaatccaat agcactggtt tgacctcagg tgtggcaggt tcggtgacgg
     1201 tgggtagaat ggcaggccgc gctgcgaatg cggctgcttt ggattatacc tggccatctt
     1261 ctgaatgtaa atgcgtatct tcagctttcg gtcagcgata tacttgcggg caagttctgc
     1321 ctgcgtaaag ccaggcatga agggtatctc ttcttcgccg acatacgaaa agttggattt
     1381 ttgcgcgacc ggcccctgcg gcccaaggtg tgatgccggg ttaggcacat cgatgaggta
     1441 gagcagctgg tcgctagcat tcgcgtgcat cgcacgcata atgcggttga gcacggtacg
     1501 gtaagtatag ggccgcaccc ccgaatcagg caggtcaaaa acgatcgcct cgcagtcttt
     1561 gattttttcc tggagttt
//
LOCUS       DAOQJF010000526         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_73346_length_1578_cov_3.015102, whole genome shotgun sequence.
ACCESSION   DAOQJF010000526 DAOQJF010000000
VERSION     DAOQJF010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73346_length_1578_cov_3.015102"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..690)
                     /locus_tag="PLF85_17400"
     CDS             complement(25..690)
                     /locus_tag="PLF85_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21498.1"
                     /translation="MKPGPQVQTPTGLIVLASGQVMPNIKSLLWAIKKANLSALAIVA
                     SGVDESQKAADRLAKLVTKINAGKIADFRTNEPVAVEVFVERDIDRFPERLSPRLTSF
                     IEKRREKIPHWMINFTAGSKIMFLSLLSHFRTSVQEWHYLEGARSGRWSRYRIDQDLS
                     ITTEELEIPDYVAGCIIGNRKTRKFHVPEGRYYNQAKESKNRVFFKNAADAEKAGYTK
                     SAR"
     gene            complement(717..1343)
                     /locus_tag="PLF85_17405"
     CDS             complement(717..1343)
                     /locus_tag="PLF85_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21499.1"
                     /translation="MKARLLILTRNKGGYQRISCGALDIENGKSYNLLEMNGDRPAFS
                     RPYHVGEVMEVDFEPAAGLRAPHTEDILVRSTERISGQLPDIAQTAEKYGPVPAGDHK
                     ALFDGRLNVTGSGSAFINTQNLVQYSASFWRASRPLIKARFQNKTRYILVSTTSKISI
                     PYMGLEVDLPPVISAGTLLRVTLGPWWRPDDSPQVEERCYLQLSAFYR"
     gene            complement(1340..>1578)
                     /locus_tag="PLF85_17410"
     CDS             complement(1340..>1578)
                     /locus_tag="PLF85_17410"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TM1812 family CRISPR-associated protein"
                     /protein_id="HNE21500.1"
                     /translation="EVVRQIAPIIKQGSEIALDITHGFRFQPMLVRDIVIGECRAKQA
                     KLKHVFYGALEMPDPETGFRPVIDLIEVLGMGPA"
BASE COUNT          323 a          405 c          440 g          410 t
ORIGIN      
        1 ataattatta attttgctaa gaatttaacg agcagatttt gtataacctg ctttttctgc
       61 atcagcagca tttttgaaga aaacgcgatt ttttgattct tttgcttggt tgtaataacg
      121 gccttctgga acatggaatt ttctagtctt gcgatttcca attatacaac ctgcaacata
      181 atcaggtatt tctagctctt cggtcgtaat cgataagtcc tggtcaatgc ggtatcggct
      241 ccacctgccc gagcgcgcgc cttcgaggta gtgccattcc tgtaccgaag ttctgaaatg
      301 cgacagcagt gacaggaaca tgatcttgct accggcggtg aaattgatca tccagtgcgg
      361 aatcttctcg cgccgctttt caatgaagct ggtcaagcgc ggcgaaagtc tttcaggaaa
      421 acgatcgatg tctctttcga caaaaacctc aacggctacc ggttcatttg tgcggaagtc
      481 ggcgatcttg ccagcgttga tctttgtgac cagctttgcg agtcggtcgg cagccttctg
      541 tgattcatcg acgccgctgg cgacaatggc gagcgcgctc aggttggcct ttttgatcgc
      601 ccacaacagg gacttgatgt tcggcattac ctggccgctt gccagaacga tcaatccagt
      661 gggagtttgt acctgggggc ctggtttcat tgcgggctgg gcctcttgcc gttagttcag
      721 cgatagaacg ccgagagctg caggtagcag cgctcttcga cctggggcga atcgtcgggc
      781 cgccaccagg ggccgagtgt tacgcgtagc agcgtgcccg cactgatcac cggcggcagg
      841 tcaacttcaa ggcccatgta gggtatcgag atcttgctcg tggtagatac aagtatgtag
      901 cgcgttttgt tctggaagcg tgctttgatg agcggtcgcg aagcccgcca gaaactcgcg
      961 ctgtattgta cgaggttttg tgtattgatg aatgccgagc cgctgccagt gacatttaat
     1021 ctgccgtcga acagcgcctt gtgatcgccg gcaggcacag ggccatactt ttccgcggtc
     1081 tgtgcaatat cgggcaactg acctgatatg cgctcggtgc tgcgtaccag aatgtcttcg
     1141 gtatgcggag cacgcaggcc tgctgcgggt tcaaagtcaa cttccatgac ctcaccgaca
     1201 tggtacggcc gcgaaaaggc ggggcgatcg ccgttcattt cgagcaggtt atagctcttg
     1261 ccgttttcga tatcgagtgc tccgcagctg atgcgctggt agccaccctt gttgcgtgtc
     1321 agaatcagca gtcgcgcttt catgctggcc ccatgccgag cacttcgatc aggtcaatta
     1381 ccggcctgaa gcccgtctcg gggtcgggca tttcgagcgc gccgtagaaa acgtgcttga
     1441 gtttcgcctg tttggcgcgg cattcgccga tcacgatgtc gcgcaccagc atcggctgaa
     1501 agcgaaaacc gtgcgtgata tcgagcgcaa tttcagaacc ctgtttgatg atgggtgcaa
     1561 tttgtcggac aacttcgt
//
LOCUS       DAOQJF010000527         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_74014_length_1571_cov_6.293536, whole genome shotgun sequence.
ACCESSION   DAOQJF010000527 DAOQJF010000000
VERSION     DAOQJF010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74014_length_1571_cov_6.293536"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLF85_17415"
     CDS             <1..431
                     /locus_tag="PLF85_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21501.1"
                     /translation="STRAGSLAFAGRFTPVFGVFARYRQFFERGVQSLHDSRFGFEVA
                     LLQFSSMQLSAQLAYVNFSGLLERSGASPALDLLFFPGKPVVFGVSVGTVQFPQITYL
                     ELRPRFGILISRWDLFVAGYLLSADSAYLRGIETGARVWF"
     gene            complement(473..1444)
                     /locus_tag="PLF85_17420"
     CDS             complement(473..1444)
                     /locus_tag="PLF85_17420"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNE21502.1"
                     /translation="MDEKYPPFSVTPKALRLVSEIERLLGQVDLSTLVRQEPMLRRQN
                     RVRSVKDSLAIEGNSLSLDQATAIFDKKRVIGPKNEIREIQNAIEAYAAAGKWRSTSE
                     RDFKKAHGIMMAGLLASVGKYRAGAVGIMKGERISHIAPGAKQLPQLMGRLFSYAERN
                     RDLHPLVMAAVLHYEIEYIHPFEDGNGRMGRLWQHLVLREYHQFFETVPFESVIKAKQ
                     KRYYRVLELCDKAGHATKFVEFALETTAIALKEVVTVTPRRRSTTKERISFAETVFKK
                     KWFSRKDYISLFADISLPTASRDLAQAVKEKAMSSRGEKNQTQYRYR"
BASE COUNT          306 a          426 c          421 g          418 t
ORIGIN      
        1 gttcgacccg cgccggcagc ctggcattcg ccggccgctt cacgcccgtc ttcggtgtct
       61 ttgcccgtta tcgacagttc tttgaacggg gagttcagtc gttacacgac agccgcttcg
      121 gtttcgaggt ggcgctgctg cagttcagct caatgcagct gagtgcgcag ctggcatatg
      181 tcaatttttc aggtctgctt gagcgctcag gtgcaagccc cgcactcgat ctgttgtttt
      241 tcccaggcaa gcccgtggtg tttggtgtct cggtcggcac cgtacagttt ccgcagatca
      301 cgtatcttga gctgcgaccg aggttcggta ttctcatcag ccggtgggat ctattcgttg
      361 ctggttatct gctctcggca gatagtgcgt acctgcgcgg catcgaaacc ggcgcaagag
      421 tttggttttg actgcagaga atccatcatg actgctattg ctggctctgg cttcaacggt
      481 aacggtactg cgtctgattt ttttcacctc gtgagctcat cgccttttcc ttcaccgcct
      541 gggcaagatc gcggcttgcc gtcggcagcg agatatcggc gaacagggaa atgtagtctt
      601 ttcggctgaa ccatttcttc ttgaaaacag tttcagcaaa cgaaattctt tctttggttg
      661 tcgaccggcg acgtggggtc acggtaacga cttctttaag ggcgatcgcc gttgtttcga
      721 gtgcgaactc cacgaatttc gtggcatgac ccgctttgtc gcagagttcg agtactcgat
      781 agtatcgttt ttgtttcgcc ttgatcacgg actcgaaggg cacggtctcg aagaattggt
      841 ggtactcgcg tagtaccagg tgctgccata gtcggcccat acggccattg ccgtcttcaa
      901 acgggtgtat gtattcaatc tcgtagtgca gcacggcagc catcaccagc ggatgcagat
      961 cgcggttgcg ctccgcgtat gaaaaaagtc tacccatcag ctgcggcagc tgtttggcgc
     1021 cgggtgcaat atgcgagatg cgttcacctt tcatgatacc cacggcgccg gcacggtatt
     1081 ttcccacaga agccaaaagg cccgccatca tgatgccgtg cgctttctta aaatctcttt
     1141 cactggtcga tcgccatttg cccgcggcgg cataggcctc gatagcattc tgtatttcgc
     1201 ggatttcatt tttggggcca ataacacgct tcttgtcaaa gatcgccgtg gcctgatcaa
     1261 gcgacaggct gttgccctca atcgcgagtg agtctttcac cgaacgcacc cggttctgcc
     1321 ggcgcagcat aggctcttgc cttacaagag tcgaaaggtc gacctggccc agaaggcgct
     1381 caatttcaga gacgagccgc agcgctttcg gtgtgacgga aaacggaggg tatttctcgt
     1441 ccataaatga tagtatcaaa tgatactatc attgtaaagc caaatttagc ggtcttcgtt
     1501 cttttggatg gcgccgccgt tacccagtcc tgataagcct caaatgctca tgcccacaac
     1561 ttctgcgggt a
//
LOCUS       DAOQJF010000528         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_76330_length_1547_cov_4.895442, whole genome shotgun sequence.
ACCESSION   DAOQJF010000528 DAOQJF010000000
VERSION     DAOQJF010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76330_length_1547_cov_4.895442"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..661
                     /locus_tag="PLF85_17425"
     CDS             182..661
                     /locus_tag="PLF85_17425"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HNE21503.1"
                     /translation="MAQAILPQQGTAVSDLLTRQNAVPARPQAQGGGPAASASPAFSS
                     LVSQIVQRGKSDRPKTVEDLMAQLDDEERDFADSPSRERFETYRKTVKLLCSMLLNRG
                     YRLQGWEDKRKRRYEVIKTIDSRLAQLYTGLLRRNQDVVIALHLMGQIRGLIFDLQA"
     gene            complement(671..>1547)
                     /locus_tag="PLF85_17430"
     CDS             complement(671..>1547)
                     /locus_tag="PLF85_17430"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21504.1"
                     /translation="EISLITASILSKKLAEDLDELVMDVKCGSGAFMQTLPAARALAK
                     SIADTARAAGVKTTCLITAMDFPLGLYTGNTIETYEAFEFCRPSGIYLETANKLYVKK
                     AKLSRDELLVQPLVEITVELAAAMLDHRARTDKQARAKAIRTLMALWQSGTLEKKMEE
                     WLAAQKGDLAGLRKKAKEILTAFVDAKSADVHVYRAPGKGYFAHADSRAIGNLMVELG
                     AGRKKSEDSVDDRVSLEMMKYRGEQCNKGDALVRIYKPGISNADRKTIDTVLKSALKV
                     GATKPKATKNVLAKM"
BASE COUNT          318 a          432 c          427 g          370 t
ORIGIN      
        1 ttcatctcat gaaaaatgcc cgtgtcatgg gagacatcgt cgcggcaaac ctgctggtcg
       61 atgaaggcgt catctttgaa ggtcgtgccc gtattaatca cctgaacgtc ggcagctaag
      121 gaataatcct gcccggttcg gtgcttcagg gaaaggcaga actagccgat attctaccgg
      181 gatggcgcag gcaattctac cacagcaggg tacagccgtc agcgatctgc tcacgcggca
      241 gaacgcggta cccgcgcgtc cacaggcgca gggtgggggg ccggcagcgt cagcatcgcc
      301 tgcattctca tcgctcgttt cacagatcgt ccagagggga aaatccgaca ggccaaagac
      361 cgtagaagac ctgatggcgc agctagacga cgaggagcgc gattttgccg attcgccctc
      421 gcgtgaacgt tttgaaacct accgcaagac agtaaaactg ctctgctcga tgctgctaaa
      481 ccgtggttac cgcctgcaag ggtgggagga caagcgcaag cggcgctacg aggtcatcaa
      541 gaccatcgac agccgcctcg cgcagcttta taccggttta cttcgccgca accaggatgt
      601 agtgatagcg ctgcacctga tgggccagat acgcggcctg atctttgacc ttcaggcctg
      661 aaacagaatc ttacattttt gccagcacgt tctttgtggc tttgggcttt gttgctccga
      721 ccttcagtgc cgatttcagg accgtatcga tggtcttacg gtctgcgttg ctgataccag
      781 gtttgtagat acgcacgagc gcatcacctt tattgcattg ttcgccgcgg tacttcatca
      841 tttcgagtga gacgcgatca tcgactgagt cctcggactt tttccggccg gctccgagtt
      901 ctaccatgag gttgccgatt gcgcggctgt cggcatgcgc gaaataccct ttgcccggcg
      961 cgcggtagac gtgcacatcg gccgattttg catcaacaaa ggctgtgaga atttctttcg
     1021 cctttttgcg caggccagcg agatcgccct tctgcgccgc gagccattct tccatttttt
     1081 tctccagcgt accagattgc cagagagcca tcagagtcct gatcgccttt gccctggcct
     1141 gcttgtcagt tcgtgcacgg tgatcgagca ttgctgccgc gagttcaacg gtgatttcta
     1201 ccaggggttg tacgagcagc tcatcgcggc tgagtttggc cttttttacg tagagtttat
     1261 tggccgtttc aaggtaaatt cccgagggcc ggcagaactc aaacgcttca taagtttcaa
     1321 tcgtattacc tgtataaagc ccgagcggaa aatccatggc agtgatcagg caggttgttt
     1381 ttacgccggc cgcccgcgcg gtatcggcaa tcgattttgc cagcgccctg gccgcaggca
     1441 gcgtctgcat aaatgccccg ctgccgcact tgacatccat cacaagctcg tccaggtctt
     1501 cggccagctt cttggagagt atcgaggcag tgatgagcga gatttcg
//
LOCUS       DAOQJF010000529         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_76331_length_1547_cov_4.281501, whole genome shotgun sequence.
ACCESSION   DAOQJF010000529 DAOQJF010000000
VERSION     DAOQJF010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76331_length_1547_cov_4.281501"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLF85_17435"
     CDS             <1..577
                     /locus_tag="PLF85_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNE21505.1"
                     /translation="STGFFSVASTCNTCGGTGKLIKNRCKSCSGSGTVAKKRKLAIRI
                     PAGIDSGTRLKISGEGEAGPRGGPAGDLYVVVQIRKHPDFERDGVDLAMFIDVPVTTA
                     ILGGEVTVETLDKSKVKVKIPAGTQPDTLFRVRGKGLPYMGGQGRFGDLIAHARIDIP
                     KSLSGKAKTLVKELEAELQASGSGIFGRFRS"
     gene            594..>1547
                     /locus_tag="PLF85_17440"
     CDS             594..>1547
                     /locus_tag="PLF85_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNE21506.1"
                     /translation="MQNQTAKKIRFGIIGVGHMGSYHLNVVSSLSTHELVGIYDARPE
                     HAAQKAEQFGTRAFASYEELIKECEAVTIAVPTHLHYEIAKKALAAGKHVLVEKPITL
                     DIAQAEELINLAEAKNLILQVGHVERFNGAVMALGNIVTEPLLIQTRRLNPFSGRILD
                     AGVVLDLLIHDIDIVVNLVGKPVVRHAAFGNCITGKHEDIAVIELQFEGGCIATLTAS
                     RLSQYKERALTVMQKNNFLILNYGTQEIEIHRQGNSVTVTAPDEIKYSQESIVERVFI
                     HKDNPLKSEHLHFYNCIVGSEKPLVTGRADLSTLKIALDSVK"
BASE COUNT          384 a          398 c          432 g          333 t
ORIGIN      
        1 cagcacggga ttcttctctg tagcttcgac ctgtaacacc tgcggtggca cgggtaaact
       61 gatcaaaaac cgctgcaaga gctgcagcgg ttcgggcacc gtggcgaaaa aacggaagct
      121 ggcaattcgt attcctgccg gcattgatag cggcacgcgc ctgaagattt cgggcgaagg
      181 cgaagcagga ccccgtggcg gccccgcggg agacctttac gtggttgtac aaatccgcaa
      241 acatcctgat tttgagcgcg acggggttga tctggcgatg ttcatcgatg tcccggtaac
      301 gacggctata ctgggcggtg aagtcaccgt cgaaacgctc gataaaagta aagtgaaggt
      361 gaaaattcct gctggcacgc agcctgatac attgttccgt gtccgcggca aaggtttgcc
      421 atacatgggt ggccagggcc gttttggtga cctgattgcg catgcgcgta ttgatatccc
      481 aaaatcgttg tcgggtaaag cgaagacact cgttaaagag ctcgaagcag aattacaggc
      541 ttccggtagc gggattttcg gccgattccg ttcgtaaatc ccgcgagata gagatgcaaa
      601 atcagactgc gaaaaaaatc cgttttggca ttattggcgt aggccacatg ggcagctacc
      661 acctcaatgt ggtctcttcc ctgtcgacac atgagctcgt gggtatttac gatgctcgtc
      721 ccgagcacgc ggcgcagaaa gccgaacaat tcggcacgcg ggcgtttgcc tcttacgaag
      781 aactgataaa agaatgcgaa gcggttacca tcgctgtccc tactcacctg cactacgaga
      841 tcgcgaaaaa ggcgctcgct gctggcaagc atgtgctggt cgaaaagccg attaccctcg
      901 acattgcgca ggcagaagag ctcatcaacc tggcagaggc taaaaacctg attctccagg
      961 ttggtcatgt cgagcgcttc aacggcgcgg tgatggccct gggcaatatt gtgaccgagc
     1021 cgctgctgat acagacgcga cggctcaacc cattcagtgg ccgcatactc gatgcgggtg
     1081 tggtgctcga cctgctgatc catgacatcg atatcgtggt gaaccttgtc ggaaaacctg
     1141 tagtgcgcca cgccgctttc ggtaattgta ttacgggcaa gcatgaagat attgccgtga
     1201 ttgaactgca gtttgaaggc ggctgtattg caacgctgac cgcatcgcgc ctgagccagt
     1261 acaaagaacg tgcgctcacg gtgatgcaga agaacaactt cctgatctta aactacggta
     1321 cgcaggaaat tgagatccac cggcagggta actctgtcac ggtgacggca cccgacgaga
     1381 tcaaatattc acaggaatcg attgttgagc gagtctttat tcacaaggat aacccactca
     1441 aaagtgaaca cctgcacttt tacaactgca tcgtcggcag cgaaaagccg ctggttacgg
     1501 gacgcgccga tctttccacg ctaaagatcg cgctggatag cgtgaaa
//
LOCUS       DAOQJF010000530         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_79073_length_1520_cov_4.438225, whole genome shotgun sequence.
ACCESSION   DAOQJF010000530 DAOQJF010000000
VERSION     DAOQJF010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79073_length_1520_cov_4.438225"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLF85_17445"
     CDS             <1..265
                     /locus_tag="PLF85_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HNE21507.1"
                     /translation="YYGLARLADGHGYPAVANLGRKPTAGEHPLGLEVHLLNFSGDLY
                     GSALRFEFRGRIRGERRFASLDELRAQIDKDVMVAQQKFVSLD"
     gene            308..781
                     /locus_tag="PLF85_17450"
     CDS             308..781
                     /locus_tag="PLF85_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21508.1"
                     /translation="MSLLFLLTVVLAPAQSRAADVVGRLLKDTVATFSDSYDLLYYSL
                     KSALRRDLREQAQAVQVTRETTNERIEAYLEAKGILIRRDSQPITRRDFARILIERFD
                     LPKGFFTRLTGSSSWYFRDAVRAGLFSEMDAGEGTMSTREMLSVFTRAEQLSRSR"
     gene            complement(796..1113)
                     /gene="trxA"
                     /locus_tag="PLF85_17455"
     CDS             complement(796..1113)
                     /gene="trxA"
                     /locus_tag="PLF85_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNE21509.1"
                     /translation="MGLVQEITDQNFGSKTSGGVVMVDFWAPWCGPCRMVAPVLEDLQ
                     KEMGEKVSILKMNVDENPETAMKFGITSIPTLMVFKNGEMVDRTVGAAPKEHYKTILG
                     KHI"
     gene            1229..>1520
                     /locus_tag="PLF85_17460"
     CDS             1229..>1520
                     /locus_tag="PLF85_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21510.1"
                     /translation="MPSGRKTARSNLRQRLKGGSAFTLAIIPASAGFKREWRVSYATV
                     WFFLAMMALVACFIAFALLNKPALEADKRLSEEAASAWLARWEVLEKGKDRIT"
BASE COUNT          316 a          424 c          396 g          384 t
ORIGIN      
        1 ctactacggc ctcgcccgtc tcgccgacgg ccatggttac ccggcagtcg cgaatctcgg
       61 ccgaaaacct acagccggtg aacacccgct cgggctcgaa gtccacctgc tcaatttctc
      121 aggggatctc tacggctcag ctctgcgttt tgagttccgt gggcgcattc gcggcgaacg
      181 caggtttgcc agcctggatg aactgcgcgc gcagattgat aaggatgtga tggtggcaca
      241 gcagaaattt gtgtcgctgg attgagtttt ttaccgtata tttaagcaaa agatgcagtt
      301 tcgcgcattg tcacttctgt tcctgctcac tgtggtgtta gccccggcac aatcacgtgc
      361 cgcggacgtg gtcggccgcc tgctcaagga taccgttgca accttttctg attcatacga
      421 cctgctttac tactcgctga aatcggcgct acggcgcgac ctgcgcgagc aggcgcaggc
      481 agttcaggtg acgcgcgaaa ccacgaacga acgtatagaa gcctacctcg aggcaaaggg
      541 aatcctgata cgccgggaca gccagccgat aacccgcaga gatttcgcgc gcattttgat
      601 agagcgcttt gatttaccaa aaggcttttt tacccgcctg accggcagca gtagttggta
      661 ttttcgcgat gcggtcaggg ctggtctttt ttctgaaatg gatgctggtg aagggacaat
      721 gtccacgcgc gagatgttat ctgtcttcac gcgcgcagaa caactgagcc gctcacgcta
      781 gagcggcccg gtttttcaga tgtgcttgcc aagaattgtt ttgtaatgct cttttggcgc
      841 ggcgccgacc gtgcgatcaa ccatctcgcc gttcttgaag accatgaggg tcggaatgct
      901 ggtaatgcca aacttcatag cagtctcggg attttcatcg acattcattt tcagaatcga
      961 tactttttct cccatttctt tttgcagatc ttccagcact ggcgccacca tgcggcaagg
     1021 accgcaccaa ggcgcccaaa agtctaccat gactaccccg cccgaggttt tgctgccgaa
     1081 attctggtct gtgatttcct gtacgaggcc cattgtaact ccttttagat gaggcaaaac
     1141 accatttggc ttgacgtttt gttttcttga acgcataata ctaaatcacc cggtgggctg
     1201 caaattattt tcccggtggt aaattccgat gccttctggc cgcaaaacag cacgttccaa
     1261 ccttcggcag cgccttaaag gcggctcggc cttcaccctt gccataattc cggcgtcagc
     1321 aggtttcaag cgcgaatggc gggtttctta tgccaccgta tggttcttcc tggccatgat
     1381 ggcgcttgtg gcctgcttca tcgcttttgc gctcttaaac aagccagccc tcgaagccga
     1441 caaacgtttg tccgaagagg ccgcttctgc ctggcttgcc cgctgggaag tgctcgaaaa
     1501 gggtaaagac cgcatcacgg
//
LOCUS       DAOQJF010000531         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_79678_length_1514_cov_3.912954, whole genome shotgun sequence.
ACCESSION   DAOQJF010000531 DAOQJF010000000
VERSION     DAOQJF010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79678_length_1514_cov_3.912954"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLF85_17465"
     CDS             complement(<1..621)
                     /locus_tag="PLF85_17465"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNE21511.1"
                     /translation="MARTNAKKTQPRKKPRSINPGRTVASIAAETPAQPTTYVSPVLQ
                     SRLPAISWYEFVSTGFFSGYLPKAPGTWGSLLAVLLFSITAKLLPNDGLWRIASLTVS
                     WWALLLGVATTAIGIYASGILAQEWREEDPGEVVIDEFAGIFFAVSLVTPDFIGLAAA
                     FAFFRLFDVWKPGPIHSLQDMPGGYGIVLDDVLAGLAAAPAALGVQY"
     gene            complement(640..>1514)
                     /locus_tag="PLF85_17470"
     CDS             complement(640..>1514)
                     /locus_tag="PLF85_17470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNE21512.1"
                     /translation="TEEGLFSPKNSFVLNSRRYSGALEAKRVGGEWLYVNHVPIDEYL
                     TSVVSHEMSPTWHREALKAQAVVARTYLLTKMKERADKPYDVDTTTNYQVYGGIKKND
                     GNARAAVAETHNEVLLHDGRLAQTFFHSSCGGTLASAQEVWGKALPYLGVQNSPYCST
                     APVYKWQIKIPFSEIGRRLALRGVKNVSVAERSASKRVRTLKIATASGIKSVRADKFR
                     VALGAIKVKSTFFGVARKGDGIILTGRGFGHGVGMCQWGAKIQADERGMGYRRILDHY
                     FPGTSLRALNGQNV"
BASE COUNT          331 a          417 c          431 g          335 t
ORIGIN      
        1 gtactgcacg ccgagcgcag ccggtgcagc ggcaagcccc gccagcacat cgtcgagtac
       61 gatgccgtag ccgccaggca tgtcctgcag cgaatgtatg ggtcctggct tccagacatc
      121 aaacaggcga aagaaagcga aagcggctgc gaggccgatg aaatcgggag tcaccagcga
      181 aacggcaaaa aagattccgg caaattcatc gatcacaact tcgcccggat cctcttcacg
      241 ccattcctgt gccagtatac cggaggcata gataccgatg gcggttgttg ccacgcccag
      301 cagcagcgcc caccaggaaa ccgtaagtga tgcgatgcgc cagagcccgt cgttgggtaa
      361 tagtttagca gttatgctga acaacaagac tgcaagcaaa gaaccccagg tacccggtgc
      421 cttgggaagg tagccggaga aaaaacccgt agagacaaat tcgtaccagc tgatcgccgg
      481 taggcgtgat tgcagtacgg gcgaaacata tgttgttggt tgcgccggtg tttctgctgc
      541 tattgaagca actgtgcggc ccgggttgat gctgcgtggc ttcttgcggg gctgtgtttt
      601 ttttgcattc gtgcgtgcca tttgaatgag ctcctgctgt cagacgttct ggccgttcaa
      661 cgcgcgcagc gatgtccctg gaaaataatg atcgagtatg cggcggtaac ccatgccccg
      721 ctcatcggcc tgaatctttg caccccactg gcacataccg acaccatgac caaaaccgcg
      781 gcctgtaaga atgatgccat ctcccttgcg cgcaacgccg aagaaggtag actttacctt
      841 gattgcacca agagcgacgc ggaacttatc agcacgcacc gattttatcc ccgaagcagt
      901 cgctattttc agcgtgcgta cgcgttttga agcgctgcgt tcagcaacac tgacattttt
      961 cactccgcgc agcgccagtc gccgcccgat ttcgctgaaa ggaattttga tctgccattt
     1021 ataaacgggg gcagttgagc agtagggtga attctgcacg ccgaggtagg gtaaagcttt
     1081 gccccagact tcctgtgccg aagcgagggt acctccgcag gatgaatgga agaaggtctg
     1141 tgcgagacgg ccgtcgtgca gcaggacttc attgtgcgtc tcggcgacag ccgcgcgcgc
     1201 gttgccatcg tttttcttta taccaccata gacctggtag tttgtagtgg tatcgacatc
     1261 gtacggtttg tcggcgcgct ctttcatttt ggtgagcagg taggtgcgcg cgaccactgc
     1321 ctgtgctttc agtgcctcgc ggtgccaggt cgggctcatt tcgtggctga ccacagaagt
     1381 caggtattcg tcgatcggca cgtggttcac atacagccat tcgccgccga cgcgtttcgc
     1441 ttcgagcgca cccgagtacc ggcgcgaatt gaggacgaaa gagttttttg ggctaaacag
     1501 cccctcttcg gtaa
//
LOCUS       DAOQJF010000532         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_0607
            NODE_80013_length_1511_cov_3.150412, whole genome shotgun sequence.
ACCESSION   DAOQJF010000532 DAOQJF010000000
VERSION     DAOQJF010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810239
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,496
            CDSs (total)                      :: 3,475
            Genes (coding)                    :: 3,454
            CDSs (with protein)               :: 3,454
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 20 of 21
            Pseudo Genes (internal stop)      :: 0 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80013_length_1511_cov_3.150412"
                     /isolate="AMR_MDS_0607"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLF85_17475"
     CDS             complement(<1..816)
                     /locus_tag="PLF85_17475"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM1812 family CRISPR-associated protein"
                     /protein_id="HNE21513.1"
                     /translation="MDSASASIVIIPVGKYSDYSSAHYSFEGVSAVAKITAGLYAKKF
                     PDAHFVFILQDTLATKHAATTDYEALCQSVAEDFRAWLNLPDSGVSLAENSFDIIVAP
                     ARGIFVDRKSGQKLCATNSQESQRAWLTHQLIGVFSRFLPAESLSVYLDTSHGINSLP
                     LAVAATLDDLLPLCAYRYGVVALKMLNSDPYWQTGQPELEINVVQQRMVYPDLPFEGY
                     SSNVLKKGVFFKAPGDNELAQSLSRKLRDIVDYTTDQDLQALLAALRFGFPLAV"
     gene            complement(879..>1511)
                     /locus_tag="PLF85_17480"
     CDS             complement(879..>1511)
                     /locus_tag="PLF85_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21514.1"
                     /translation="QPPFEGFLSFYSSIVEHLLSLLLERTTSLALLAGGVRLGGQPVK
                     WSVRRQAFSEADIVREGLLDLALPSLVHRVQQLARANLADYRYKFVLYLKETRAADGT
                     VMRPVTGHNNRPAREILYIEQSDETEQPAAAQALITLLARREALMVIVDAATPENAWF
                     AYSPAAASIALAHVKQATGRPDATFADFVQILPVSAGQAEVVDVMLNGEW"
BASE COUNT          334 a          437 c          426 g          314 t
ORIGIN      
        1 gactgccagc ggaaagccaa agcgcagggc tgccagaaga gcctgcaggt cctggtcagt
       61 cgtataatcc acaatatctc tgagtttcct gctcaatgac tgcgccagtt cgttgtcgcc
      121 cggtgccttg aaaaaaacgc ctttcttcag gacgttgctg ctataaccct cgaatggcag
      181 atccggatag accatacgct gctgcaccac gttgatctcg agctccggct gcccggtttg
      241 ccagtacgga tcggagttga gcattttcaa cgccacgacc ccgtagcggt acgcacacag
      301 cggcagtaag tcgtcgagcg tggcagcgac ggcgagcggc aggctgttga tgccgtgcga
      361 agtatcgaga tagaccgaaa gcgactctgc aggcagaaac cgcgagaaga caccgatcag
      421 ctggtgcgta agccaggcgc gctggctttc ctgtgaattg gtggcgcaca gcttctggcc
      481 gcttttcctg tcgacgaaga ttcccctggc cggcgcgaca atgatgtcaa agctgttctc
      541 tgcgagactg acacccgaat caggcaaatt cagccaggca cgaaaatctt ccgcaaccga
      601 ttggcaaagc gcctcgtaat cagtggtcgc ggcgtgttta gtcgcaagcg tatcctgaag
      661 aataaaaaca aaatgtgcgt cgggaaattt tttggcgtaa aggcctgcgg tgatttttgc
      721 caccgcgctc acaccttcaa aactgtaatg tgcactgctg tagtcagaat atttaccgac
      781 cgggataatg acaatggaag cactggcaga gtccatgggt acatttcacc attttcgcag
      841 aagctggcaa gcgctgcagt gcttatcagc gcgtccccct accattcccc attcaacatc
      901 acgtccacca cctctgcctg cccggcgctg acgggcaata tctgcacaaa gtcggcgaat
      961 gtcgcatcgg gcctgcccgt cgcctgcttc acatgggcga gcgcaatgct agccgcggcc
     1021 gggctatagg caaaccaggc attctcgggc gttgcggcat cgacgatgac catcaatgct
     1081 tcgcggcgtg ccaacagggt gataagcgcc tgcgccgctg caggctgttc ggtttcgtcg
     1141 gattgttcga tgtagagtat ctcgcgcgca ggtcgattat tgtgtcctgt gaccgggcgc
     1201 atcacagtcc cgtcggcggc gcgggtttct ttcaggtaga ggacaaactt gtagcgatag
     1261 tccgcaaggt tagcccgtgc aagctgctgt accctgtgca cgagactggg caacgccaga
     1321 tcgagcaagc cctcacgaac tatatctgct tcgctgaagg cctgccttct caccgaccat
     1381 ttgacgggtt ggccgcccag cctgactccg ccagcgagca gcgccagcga cgttgtccgc
     1441 tccaacagca gcgaaagcag gtgctcgacg atcgagctat agaacgagag aaatccctca
     1501 aagggtggct g
//
